LOCUS       DAJTNA010000001        69767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTNA010000001 DAJTNA010000000
VERSION     DAJTNA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69767
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.001"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..257
                     /locus_tag="OO161_000001"
     CDS             <1..257
                     /locus_tag="OO161_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5461220.1"
                     /translation="LDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNGLSVV
                     MTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            267..1641
                     /gene="gspE"
                     /locus_tag="OO161_000002"
                     /pseudo
     CDS             267..1641
                     /gene="gspE"
                     /locus_tag="OO161_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1631..2817
                     /gene="hofC"
                     /locus_tag="OO161_000003"
                     /pseudo
     CDS             1631..2817
                     /gene="hofC"
                     /locus_tag="OO161_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(2852..3895)
                     /gene="guaC"
                     /locus_tag="OO161_000004"
     CDS             complement(2852..3895)
                     /gene="guaC"
                     /locus_tag="OO161_000004"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5461221.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGTDIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(4051..4095)
                     /locus_tag="OO161_000005"
     CDS             complement(4051..4095)
                     /locus_tag="OO161_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461222.1"
                     /translation="MIIKTLKMSARKRN"
     gene            4120..4740
                     /gene="coaE"
                     /locus_tag="OO161_000006"
     CDS             4120..4740
                     /gene="coaE"
                     /locus_tag="OO161_000006"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5461223.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4740..5483
                     /gene="zapD"
                     /locus_tag="OO161_000007"
     CDS             4740..5483
                     /gene="zapD"
                     /locus_tag="OO161_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5461224.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5493..5690
                     /gene="yacG"
                     /locus_tag="OO161_000008"
     CDS             5493..5690
                     /gene="yacG"
                     /locus_tag="OO161_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5461225.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5797..6186)
                     /gene="mutT"
                     /locus_tag="OO161_000009"
     CDS             complement(5797..6186)
                     /gene="mutT"
                     /locus_tag="OO161_000009"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5461226.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(6246..8951)
                     /gene="secA"
                     /locus_tag="OO161_000010"
     CDS             complement(6246..8951)
                     /gene="secA"
                     /locus_tag="OO161_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5461227.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(9013..9525)
                     /gene="secM"
                     /locus_tag="OO161_000011"
     CDS             complement(9013..9525)
                     /gene="secM"
                     /locus_tag="OO161_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5461228.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9755..10672)
                     /gene="lpxC"
                     /locus_tag="OO161_000012"
     CDS             complement(9755..10672)
                     /gene="lpxC"
                     /locus_tag="OO161_000012"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5461229.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(10773..11924)
                     /gene="ftsZ"
                     /locus_tag="OO161_000013"
     CDS             complement(10773..11924)
                     /gene="ftsZ"
                     /locus_tag="OO161_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5461230.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(11985..13247)
                     /gene="ftsA"
                     /locus_tag="OO161_000014"
     CDS             complement(11985..13247)
                     /gene="ftsA"
                     /locus_tag="OO161_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5461231.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(13244..14074)
                     /gene="ftsQ"
                     /locus_tag="OO161_000015"
     CDS             complement(13244..14074)
                     /gene="ftsQ"
                     /locus_tag="OO161_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5461232.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(14076..14996)
                     /gene="ddlB"
                     /locus_tag="OO161_000016"
     CDS             complement(14076..14996)
                     /gene="ddlB"
                     /locus_tag="OO161_000016"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5461233.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(14989..16464)
                     /gene="murC"
                     /locus_tag="OO161_000017"
     CDS             complement(14989..16464)
                     /gene="murC"
                     /locus_tag="OO161_000017"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5461234.1"
                     /translation="MNTQQLAKLRTIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16518..17585)
                     /gene="murG"
                     /locus_tag="OO161_000018"
     CDS             complement(16518..17585)
                     /gene="murG"
                     /locus_tag="OO161_000018"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5461235.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17582..18826)
                     /gene="ftsW"
                     /locus_tag="OO161_000019"
     CDS             complement(17582..18826)
                     /gene="ftsW"
                     /locus_tag="OO161_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5461236.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18826..20142)
                     /gene="murD"
                     /locus_tag="OO161_000020"
     CDS             complement(18826..20142)
                     /gene="murD"
                     /locus_tag="OO161_000020"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5461237.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(20145..21227)
                     /gene="mraY"
                     /locus_tag="OO161_000021"
     CDS             complement(20145..21227)
                     /gene="mraY"
                     /locus_tag="OO161_000021"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5461238.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(21221..22579)
                     /gene="murF"
                     /locus_tag="OO161_000022"
     CDS             complement(21221..22579)
                     /gene="murF"
                     /locus_tag="OO161_000022"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5461239.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22576..24063)
                     /gene="murE"
                     /locus_tag="OO161_000023"
     CDS             complement(22576..24063)
                     /gene="murE"
                     /locus_tag="OO161_000023"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5461240.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQYRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINLNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(24050..25816)
                     /gene="ftsI"
                     /locus_tag="OO161_000024"
     CDS             complement(24050..25816)
                     /gene="ftsI"
                     /locus_tag="OO161_000024"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5461241.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGSDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25832..26197)
                     /gene="ftsL"
                     /locus_tag="OO161_000025"
     CDS             complement(25832..26197)
                     /gene="ftsL"
                     /locus_tag="OO161_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5461242.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(26194..27135)
                     /gene="rsmH"
                     /locus_tag="OO161_000026"
     CDS             complement(26194..27135)
                     /gene="rsmH"
                     /locus_tag="OO161_000026"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5461243.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIACAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(27137..27595)
                     /gene="mraZ"
                     /locus_tag="OO161_000027"
     CDS             complement(27137..27595)
                     /gene="mraZ"
                     /locus_tag="OO161_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5461244.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEVIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGNLSERLQDLSL"
     gene            complement(28197..29201)
                     /gene="cra"
                     /locus_tag="OO161_000028"
     CDS             complement(28197..29201)
                     /gene="cra"
                     /locus_tag="OO161_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5461245.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29283..29369)
                     /locus_tag="OO161_000029"
     CDS             complement(29283..29369)
                     /locus_tag="OO161_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5461246.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29381..29872)
                     /gene="ilvN"
                     /locus_tag="OO161_000030"
     CDS             complement(29381..29872)
                     /gene="ilvN"
                     /locus_tag="OO161_000030"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5461247.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLALIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29875..31599)
                     /gene="ilvI"
                     /locus_tag="OO161_000031"
     CDS             complement(29875..31599)
                     /gene="ilvI"
                     /locus_tag="OO161_000031"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5461248.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            31718..31762
                     /locus_tag="OO161_000032"
     CDS             31718..31762
                     /locus_tag="OO161_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461249.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(31917..32861)
                     /gene="leuO"
                     /locus_tag="OO161_000033"
     CDS             complement(31917..32861)
                     /gene="leuO"
                     /locus_tag="OO161_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5461250.1"
                     /translation="MPEVQTDHSETVELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            33522..33608
                     /gene="leuL"
                     /locus_tag="OO161_000034"
     CDS             33522..33608
                     /gene="leuL"
                     /locus_tag="OO161_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5461251.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            33701..35272
                     /gene="leuA"
                     /locus_tag="OO161_000035"
     CDS             33701..35272
                     /gene="leuA"
                     /locus_tag="OO161_000035"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5461252.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDVVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            35272..36363
                     /gene="leuB"
                     /locus_tag="OO161_000036"
     CDS             35272..36363
                     /gene="leuB"
                     /locus_tag="OO161_000036"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5461253.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAAYAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            36366..37766
                     /gene="leuC"
                     /locus_tag="OO161_000037"
     CDS             36366..37766
                     /gene="leuC"
                     /locus_tag="OO161_000037"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5461254.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEVATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            37777..38382
                     /gene="leuD"
                     /locus_tag="OO161_000038"
     CDS             37777..38382
                     /gene="leuD"
                     /locus_tag="OO161_000038"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5461255.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(38431..39513)
                     /locus_tag="OO161_000039"
     CDS             complement(38431..39513)
                     /locus_tag="OO161_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5461256.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMIGGAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(39615..39746)
                     /gene="sgrT"
                     /locus_tag="OO161_000040"
     CDS             complement(39615..39746)
                     /gene="sgrT"
                     /locus_tag="OO161_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5461257.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            39835..41490
                     /gene="sgrR"
                     /locus_tag="OO161_000041"
     CDS             39835..41490
                     /gene="sgrR"
                     /locus_tag="OO161_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5461258.1"
                     /translation="MSSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      41524..41623
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            41654..42637
                     /gene="thiB"
                     /locus_tag="OO161_000042"
     CDS             41654..42637
                     /gene="thiB"
                     /locus_tag="OO161_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5461259.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVQFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            42613..44223
                     /gene="thiP"
                     /locus_tag="OO161_000043"
     CDS             42613..44223
                     /gene="thiP"
                     /locus_tag="OO161_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5461260.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            44207..44905
                     /gene="thiQ"
                     /locus_tag="OO161_000044"
     CDS             44207..44905
                     /gene="thiQ"
                     /locus_tag="OO161_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5461261.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(45019..45783)
                     /locus_tag="OO161_000045"
     CDS             complement(45019..45783)
                     /locus_tag="OO161_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5461262.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(45869..46747)
                     /gene="araC"
                     /locus_tag="OO161_000046"
     CDS             complement(45869..46747)
                     /gene="araC"
                     /locus_tag="OO161_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5461263.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFVNTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            47085..48785
                     /gene="araB"
                     /locus_tag="OO161_000047"
     CDS             47085..48785
                     /gene="araB"
                     /locus_tag="OO161_000047"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5461264.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSLEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            48796..50298
                     /gene="araA"
                     /locus_tag="OO161_000048"
     CDS             48796..50298
                     /gene="araA"
                     /locus_tag="OO161_000048"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5461265.1"
                     /translation="MTIFDNYDVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            50498..51193
                     /gene="araD"
                     /locus_tag="OO161_000049"
     CDS             50498..51193
                     /gene="araD"
                     /locus_tag="OO161_000049"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5461266.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            51268..53619
                     /gene="polB"
                     /locus_tag="OO161_000050"
     CDS             51268..53619
                     /gene="polB"
                     /locus_tag="OO161_000050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5461267.1"
                     /translation="MAQAGFILTRHWRDTPQRTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     NCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            53783..56689
                     /gene="rapA"
                     /locus_tag="OO161_000051"
     CDS             53783..56689
                     /gene="rapA"
                     /locus_tag="OO161_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5461268.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKMVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            56701..57360
                     /gene="rluA"
                     /locus_tag="OO161_000052"
     CDS             56701..57360
                     /gene="rluA"
                     /locus_tag="OO161_000052"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5461269.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAPDNTARVVLKPITGR
                     SHQLRVHMLAQGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(57477..58292)
                     /gene="djlA"
                     /locus_tag="OO161_000053"
     CDS             complement(57477..58292)
                     /gene="djlA"
                     /locus_tag="OO161_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5461270.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            58547..60901
                     /gene="lptD"
                     /locus_tag="OO161_000054"
     CDS             58547..60901
                     /gene="lptD"
                     /locus_tag="OO161_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5461271.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            60954..62240
                     /gene="surA"
                     /locus_tag="OO161_000055"
     CDS             60954..62240
                     /gene="surA"
                     /locus_tag="OO161_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5461272.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            62240..63229
                     /gene="pdxA"
                     /locus_tag="OO161_000056"
     CDS             62240..63229
                     /gene="pdxA"
                     /locus_tag="OO161_000056"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5461273.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            63226..64047
                     /gene="rsmA"
                     /locus_tag="OO161_000057"
     CDS             63226..64047
                     /gene="rsmA"
                     /locus_tag="OO161_000057"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5461274.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            64050..64427
                     /gene="apaG"
                     /locus_tag="OO161_000058"
     CDS             64050..64427
                     /gene="apaG"
                     /locus_tag="OO161_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5461275.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            64434..65276
                     /gene="apaH"
                     /locus_tag="OO161_000059"
     CDS             64434..65276
                     /gene="apaH"
                     /locus_tag="OO161_000059"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5461276.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(65354..65833)
                     /gene="folA"
                     /locus_tag="OO161_000060"
     CDS             complement(65354..65833)
                     /gene="folA"
                     /locus_tag="OO161_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5461277.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(66025..67887)
                     /gene="kefC"
                     /locus_tag="OO161_000061"
     CDS             complement(66025..67887)
                     /gene="kefC"
                     /locus_tag="OO161_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5461278.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAEVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMSDEPETKPSS"
     gene            complement(67880..68409)
                     /gene="kefF"
                     /locus_tag="OO161_000062"
                     /pseudo
     CDS             complement(67880..68409)
                     /gene="kefF"
                     /locus_tag="OO161_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
     gene            complement(68517..69767)
                     /locus_tag="OO161_000063"
                     /pseudo
     CDS             complement(68517..69767)
                     /locus_tag="OO161_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT        16862 a        19331 c        17704 g        15870 t
ORIGIN      
        1 gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc acccgctatg
       61 tttcagccat gagtgtggca aaaggcgtgg tgtcgctgac cgggcaagaa agtctcaatg
      121 gactaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc ggctgggcgc
      181 gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat gtcttccgct
      241 ttgatgacgc caactaagga gcggcaatga atattccaca gctcaccgcc ctgtgtctgc
      301 gttatcaggg agtcttgctg gatgccagcg aagaggtggt tcatgttgcg gtagtcgatg
      361 caccttcgca tgagttgctg gacgcattgc atttcgctac caccaaacgt attgagatca
      421 cctgctggac gcgccaacaa atggaaggcc acgccagtcg cacacaacag acattgcccg
      481 tagctgttca ggagaagcat cagtcgaaag cagagttgct gactcgaacg ttacaatctg
      541 cgctggaaca acgtgcgtct gatattcata tcgaaccagc ggacaatgcc taccgcatcc
      601 gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat gccggagtcg
      661 cattaaccgc cagattaaaa gtgctgggaa acctggatat tgcggaacat cgcctgccgc
      721 aggacgggca attcactgtc gaactggcag gaaacgccgt ctcatttcgt attgcgacct
      781 taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg aaccaggcac
      841 tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct catgccttgc
      901 aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa acggtcacgc
      961 tttatagtgc cctgcaaaag ctgaataccg ctgacattaa tatttgtagc gtcgaagatc
     1021 cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt gccgggctca
     1081 cctttcaggg cgttttgcgt gcgttattgc gccaggatcc tgacgtcatc atgatccgat
     1141 ggcgaaacgg cagaaattgc cattaaagcc gcgcaaaccg gtcacctggt gttgtctacc
     1201 ctacacacta attccacctg cgaaacgctg gtacgtttac agcaaatggg agtcgcccgc
     1261 tggatgctct catcagcgct tacgctggta atagcccagc gtctggtacg taaattttgc
     1321 ccacattgtc gccagcagca aggggagccc atccatattc cagacaatgt atggccgtcg
     1381 ccgctgcccc actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgt
     1441 accgccttgt ttgaagtcct gcccattaca ccagccatac gccagcttat ttccgctaat
     1501 accgacgttg aatcgctgga aacgcacgca cgacaggcgg gtatgcgtac gctttttgaa
     1561 aacggctgcc tagccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg
     1621 gggatgccgc atggcgagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa
     1681 tgcgcaagat gggatgctat gggcagagag ccgtgctttg ctgctcatgg cactacagca
     1741 acagatggtt accccactta gcctgaagcg aatcgccatc aattctgcgc agtggcgagg
     1801 agataaaagc gcggaagtca ttcatcaact ggcgacgcta ctcaaagccg ggttaacgct
     1861 ttctgaaggg ctggcactgc tggcggaaca gcatcccagt aagcaatggc aagcgttgct
     1921 gcaatcgctg gcgcacgatc tcgaacaggg cattgctttt tccaatgcct tattaccctg
     1981 gtcagaggta tttccgccac tctatcgggc gatgatccgc acgggtgaac tgaccggtaa
     2041 gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc agttgaccga
     2101 caaagtgaaa tcagcgttac gttatcccat catcatttta gcgatggcaa tcatggtggt
     2161 tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atctataaga ccttcaacac
     2221 cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatggag
     2281 ctggctgctg gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg
     2341 accgacctgg cttatagtgc ggcagaaatt gctgttacgc atcccgatta tgggttcact
     2401 gatgcgggga tctttacgat tctggcgctg acacaaagtg caggcattac ttttttgcag
     2461 ggcgtagaga gcgtcagaga aacaatgcgc tgcccgtact gggtgcaact tctgacacaa
     2521 atccagcacg atatcagtaa cggtcatccc atctggctgg cgctaaaaaa taccggggag
     2581 tttagcccgc tctgtttgca attagtgaga acaggagagg catccggctc gctggacctc
     2641 atgttagaca acctcgccca tcatcatcgg gataacacaa tggcgctggc ggataacctc
     2701 gcagccttac tggaaccggc gttgctgatc ataacgggag gaattatcgg tacgctggtg
     2761 gtggcgatgt atctgccaat tttccattta ggcgatgcga tgagtgggat gggataacgc
     2821 tggcgtgttg ctccacacca gcgttggaag attacaggtt gttgaagatg cggttttctt
     2881 gttcctgcac acggataaat gtggtgcgct tggtcagttc tttcaggcgt gaagccccaa
     2941 cgtatgtaca agctgaacgc aggccgccca aaatatcacg cgcggtattt tcaaccgggc
     3001 ctcgcagcgg cagcttaacg gttttacctt ctgctgcgcg atattccgca acgccgccaa
     3061 cgtgacgttt catcgcagac tcggagctca tgccgtagaa cagcataaat ttctcgccgt
     3121 tctcctcaac gatgcgaccg ccgctctctt cgtggcccgc cagcatgccg ccaagcatga
     3181 cgaaatcggc accgccgcca aaggcttttg cgacatcgcc cggcgtggtg cagccaccgt
     3241 cgctgacgat cattccgccc agaccgtgcg ccgcatcggc acattcgatt accgcagaaa
     3301 gctgcggata accgacgcct gttttgacgc gagttgtaca aacagaacct gggccaatgc
     3361 caactttaac gatatcagta ccggagagga taagctcctc acacatttca ccagtcacta
     3421 cgttgccagc acaaatggtt ttggtcggcc acgcttcacg cgctttcgca acgaactgca
     3481 cgaagtgttc ggaataacca ttcgccacat caatacaaac aaagtttaat gccgggttca
     3541 ggtcgagaat ctgtttagtt ttttcgaaat ccgcatcaga cgtaccggta gaaaccatca
     3601 catgtttcag cacatcagcg gaagaattgt tgataaacgc ttgccactct tcgacagaat
     3661 agtgtttatg cacagcagtc aaaatatcaa aagaagccag cgcagaggcc atagaaaatg
     3721 tacctacggt gtccatattt gcggcgataa tcggcacgcc ggaccagctc tgatctgaat
     3781 gtttgaaggt gaattgacgt tccagttcaa catcggaacg gcttttaaga gtggagcgtt
     3841 tagggcggat gagaacgtct ttaaaaccta acttcagatc ttcttcaata cgcatgtgcg
     3901 gattcctggg gttaatggct ataagggtaa gagcgcaact ccagtgacgc tatcatacgc
     3961 actaatcaat gcggcgcaag actgcgaaat tgtctttttt tacgctacaa tcccataaat
     4021 ttacctggta aatagtgagc gaacgtttca tcaatttcgc tttcgtgcac tcatttttaa
     4081 cgttttgata atcatgatga attcccggga atataaatta tgaggtatat agttgcctta
     4141 acgggaggca ttggcagtgg caagagtacc gttgccaatg cgtttgctga tctcggaatt
     4201 aacgtcattg atgccgatat tattgcgcgt caggtggttg aaccaggtgc acctgcgcta
     4261 catgccattg ctgatcactt tggcgctaac atgattgctg ctgatggaac attgcagcgc
     4321 cgggccttgc gcgagcggat cttcgccaac ccggaagaga aaaactggct taacgccctg
     4381 ctgcatccgc tgattcagca agagacgcaa caccagatcc agcaagctac ttccccctat
     4441 gtactgtggg ttgtgccatt gctggtagaa aactcactgt ataaaaaagc gaatcgagtg
     4501 ctggtggtgg atgtcagccc agaaacgcaa cttaagcgca ccatgcagcg cgatgatgta
     4561 actcgcgagc atgtcgaaca aatccttgct gctcaggcaa cgcgcgaagc ccgccttgcc
     4621 gtggcagatg acgtcattga taataacggc gcaccggatg ctatcgcatc ggatgttgcc
     4681 cgcctgcacg cacactattt gcagcttgcg tcgcagtttg tctcacagga aaaaccgtaa
     4741 tgcagaccca ggtccttttt gaacatccac taaatgaaaa aatgcgtaca tggctgcgca
     4801 ttgagttttt gattcagcaa ctcaccgtta atttacccat cgttgaccac gctggcgcgc
     4861 tgcatttctt ccgtaatgtc agtgaattac tggatgtttt cgagcgcggc gaagtccgca
     4921 ctgagctgtt gaaagaactt gaccggcagc aacgtaaact ccagacctgg attggcgtgc
     4981 ctggcgtgga ccagagccgt attgaagcat taattcagca gttaaaagcg gcggggagcg
     5041 tattaatttc cgcgccgcgt atcgggcaat ttctgcgtga agatcgtttg attgctctgg
     5101 tgcgtcagcg actgagcatc ccaggcggct gttgcagctt tgatttacct acattgcaca
     5161 tttggctgca tctaccccag gcgcagcgcg acagccaggt agaaacctgg attgccagcc
     5221 tgaacccgct cacccaggca cttaccatgg tgctggattt aattcgccag tcggccccct
     5281 tccgtaaaca aaccagcctg aatggttttt atcaggataa cggtggcgat gccgacttgc
     5341 tgcgcctgaa tctgtcgctc gattcacagc tttatccgca aatttccgga cataagagcc
     5401 gttttgccat tcgttttatg ccgctggaca gtgaaaacgg acaggtaccg gaacgtctag
     5461 atttcgaact ggcctgttgc taaggagtga aaatgtcaga aactattacg gtgaattgcc
     5521 caacctgcgg gaaaacggtg gtgtggggtg aaatcagccc gtttcggcca ttttgctcca
     5581 aacgttgtca gctgatcgac ctcggagaat gggctgctga agaaaaacga atccccagca
     5641 gtggcgatct ttccgaaagc gatgactgga gcgaagagcc aaagcagtga cattggtctg
     5701 atgccgcaca taggcctgat aagacgcgga cagcgtcgca tcaggcatct tgtgcaaatg
     5761 tcggatgcgg cgtgaacgcc ttatccgacc tacagactac agacgcttaa gcttcgcaat
     5821 taccggttca ttggctggcg gaaaatcatc ggcattaaga ccgaccagcg acatccactc
     5881 accgggttgc ccttctttac cccacggcac cccttcccaa ctttcgacca gccagaacca
     5941 cagagttata tgcctgtccg ggaattcata tgccagtttt tcaaatagcg aaaaatgttg
     6001 tggggtaatc ccgacctctt cctgaagttc acgcaccacc gcctgttccg gcgtttcgcc
     6061 catttcaatt ttaccgccgg gaaactccag tttattcgcc atgtgcgcat ctgctgcgcg
     6121 acgcgttata aagatttcat tgttctcgtt gcgaataata cctaccgcaa tttgcagctt
     6181 tttcattgtt ttaaacctat aaaaaaggcg cagaatcctg cgccttttgc ttcaacagtt
     6241 agcttttatt gcaggcggcc atgacactgc ttgtattttt taccagaacc gcacgggcaa
     6301 ggatcgttac gtcctacttt gcgctcaccg gtttgcgccg ccagtgcagc tgcggctgca
     6361 gagtcgtcat cctgatggct aagctgctgc atttgcgcta aacgctcggc ttccatacga
     6421 cgctgttgtt ccagctcctc aacctcttca ggcatacgta cctgaacttt gctcagcgta
     6481 ctgataactt catatttcaa cgactccagc atcgctgcaa acatggagaa cgattcacgt
     6541 ttgtattcct gcttcggatc tttctgtgca tagccacgca ggtggatacc ctgacgcaga
     6601 tagtccatcg ctgccaggtg ctctttccac agggagtcaa gcgtttgcag catgacgcct
     6661 ttctcgaagt gacgcatcat ctcagcacca accacttctt ctttacgctg atacacttcg
     6721 atggactgcg ccagaatgcg ctcacgcagc gtctcttcat gcagttctgg ttctttatcc
     6781 agccactcgg caattggtaa atcgaggtcg aaatcgttct tcagacgttc ctgcagcccc
     6841 ggaatatccc acatttcttc cagcgactgt ggcggaatgt aggcatcaat ggtggctttg
     6901 aacacatctt cacgaatgct gttgatggtt tcgctcacat cgctgacatc cagcagttcg
     6961 ttacgctggg agtaaatggc gcgacgctga tcgttagcca cgtcatcata ttccagcagt
     7021 tgcttacgaa tgtcgaagtt acgactttca actttacgct gggcgttggc aatcgcttta
     7081 gtcacccacg ggtgttcaat ggcttcgcct ggcttcatac ccagtttacg catcatgccg
     7141 gatactcggt cggaagcaaa aatacgcatc agcgcatctt ccatcgacag gtagaaacgg
     7201 gaagaaccag catccccctg acgaccagaa cgaccgcgca actggttatc gatacgacgg
     7261 gattcgtgac gctcggtacc gatgatatgc aggccacctg cttccagtac cgcatcgtga
     7321 cgtacctgcc agtcggcttt aattttttca atttgctctg cggtcggatt ttccagcgcg
     7381 gcaacttctg cctgccagct accaccgagc acaatatctg taccacgacc cgccatattg
     7441 gtcgcgatag tcaccgcagc cggataacct gcctgagcaa caatcgccgc ttcgttggcg
     7501 tggaatttgg cgttcaggac gttgtgctta ataccggctt tggtcagttc gtttgacacc
     7561 agctccgatt tttcgatgga gatagtaccc accagcaccg gctggccttt cgcagtacgt
     7621 tctttgatat cttcaatgat cgcctgaatt ttttccgctt cagtcatgta gaccaggtcc
     7681 ggcagatctt tacgaatcat tggacggttg gtcggaacaa cgacggtatc cagcttgtag
     7741 atggagctaa attcgaaagc ttcggtatca gcagtaccgg tcatccccgc cagtttttca
     7801 tacagacgga agtagttctg gaaggtgatc gaagccagcg tttggttttc gttctggatc
     7861 tgcacacctt ctttcgcttc cacagcctgg tgcagaccat cggaccagcg acggccctgc
     7921 atggtacgac cggtgtgttc gtcaacgatg ataacttcac catctttaac gatgtagtcg
     7981 acgtcgcggg taaacagcgc atgagcgcgc agcgccgccg ttacgtggtg catcagcatg
     8041 atgttggccg gagagtacag agactcccct tcatccatga tgccctcttt caccagcagt
     8101 tcttcaatca gcaccagacc acgttcggtc aggttcacct ggcgagattt ttcgtccacc
     8161 gagaagtggc cttcgccctg gaaggtttcg gagtcttctt tttcctgacg gatcaggtgc
     8221 ggaataattt tattcacgcg tttatacatt tccgagctgt cttctgccgg gccggaaatg
     8281 atcagcggtg tacgcgcttc atcgatcagg atggagtcca cttcgtccac cagcgcatag
     8341 tgcagtttac gctgtacacg ttcttcaggg ctgaacgcca tattgtcgcg caggtagtca
     8401 aagccgtatt cgttgttcgt accgtaagtg atgtcagctg cgtaagcttc gcgctttgcc
     8461 ggtgctggca tgcccggcag gttgataccg acagtcaggc caaggaattc aaacagcgga
     8521 cggttgtttt cggcgtcacg ttgcgccagg tagtcgttaa cggtaactac gtgcacgcct
     8581 ttaccggtta gtgcgttcag gtaagcaggc agcgttgcgg tcagggtttt accttcaccg
     8641 gtacgcattt cggcgatgca gcgttcgtta agaaccatac cgccgagtaa ctgaacgtcg
     8701 aagtgacgca taccaaagac gcgcttactt gcctcacgta ccacggcgaa agcttccggg
     8761 atcagatttt ccagcacttc gcctttttcc agacgtgcac gaaactctgc ggttttccct
     8821 ttcagttctt cgtcggagag tttttccatc tccggttcca tggcattgat gatgttgacc
     8881 actttgcgca tccggcgcag ggtgcgatcg ttacgactac cgaaaacttt agttaacaat
     8941 ttgattagca taataaaatc tcaaacgccc cgcgttgcgg agttaataaa atgaagtaaa
     9001 ggtttattgt tgttaggtga ggcgttgagg gccagcacgg atgccttgcg cctggcttat
     9061 ccagacgggc gtgctgaatt ttgcttgtgg ggtaaaatgc gcataatcaa tgcgataacc
     9121 cttttcagac ggcgtgcctt cctgggtcag cagcgcgctg agcgtatcca gtaatgcaag
     9181 atgttgcgcc tgaagaggca aagattcttc agcaacgggc agtgtttgcg gtgccattgc
     9241 gaaagaaaga tgacggatta ccgtgcgaat ggcatattga tgccagtaat caacggaata
     9301 gttcgaattc gggcggcgtg tgttcgcttc cagcaaggcc aattgaccaa agttaacttt
     9361 ggctgaaggc tcgtggttgc gggttgtcgc ttttgcgggc acgtttggtt cggcggcgtt
     9421 gctgagcgca ggcaaaccta aactcgccgc gaccatccct aataagagat gcggccagaa
     9481 gtagcgttta ccaaactgtc gccagcgcgt cagtattcca ctcacgttat tgccatcccg
     9541 atcgaattca aacgtattta gcgcacaaat cttatcatta aagcctgaag cccacagcat
     9601 gaatgaagat aagttgtgcg taaaagtgct taagaaaaca gcgttcgcac cgttttttat
     9661 agctcttata aaattcaaaa aaaacgactg gttcacctgg ccggagagag tgccagattt
     9721 gccagtcgaa ttttatacga cagtataaat gtcgttatgc cagtacagct gaaggcgctt
     9781 tgaaggccaa cggcagttct gcgtcgtcct ggaaggtcac atattcccag gcttcctgtt
     9841 tcgccaggac agcctgcagc agtttgttat tcagtgcatg accggattta taagcgataa
     9901 atgcaccaat aatattgtga ccacacatga acaagtcacc gatcgcatcg agcattttgt
     9961 gacgcacaaa ttcgtcttca aaacgcaggc cgtcttcgtt cagtacgcga taatcgtcaa
    10021 caacgatggc acaatcgaag ctgccgccca ggcacaaacc acgggactgc agatattcga
    10081 tatcacgcat gaaaccgaac gtacgcgcac ggctgatctg gcgcataaac gcatcagcgg
    10141 agaagttcat cgcatagcgc tggttgctgg aatcaatagc cggatggtta aaatcgatgg
    10201 tgaaatccag cgaaaaacca ttgtacggct taaattcagc ccacttatcg ccatcttcga
    10261 cacgaacagt ctctttgatg cgaacaaatt ttttggcgca gttcaactcg tcgataccgg
    10321 cgtcaagcag caggtataca aacggagcgg cgctgccgtc catgatcggg atttccggcg
    10381 cgttaacttc gataacaatg ttatcgatgc ccaggcccgc gagagcagca ttgaggtgct
    10441 ctacggttga aatccgtaca tcatgctcgt tgaccagaca cgtacagagc atggtatcac
    10501 gcacagattt ggcatcggcc gggaaatcta ccggtggatt caagtcggtg cgacgataga
    10561 tgaccccggt gttggccggc gcagggcgta acgtcagagt gactttcttg ccggtatgta
    10621 aaccgacacc cgtcgcctga acgatacgtt taagtgtcct ttgtttgatc atcgtattat
    10681 ctcgccaaat tacctatcta accgaagtgt actatacatt cggcgggcca gtttagcaca
    10741 aagagcctcg aaaccaaaat tccagtcaat tcttaatcag cttgcttacg caggaatgct
    10801 gggatatcca gataatccgg ctctttcgca gtttgcggcg cattgtcatt cacgacttta
    10861 gcaaccggct tctgctcctg ggtcagcgga gccatcccat gctgctggta gcgatccatc
    10921 actggctgct gaacctgctt attggtcacc agagtgattt caggacgttt gtccatgccg
    10981 atacctgtcg caacaacggt tacgcgcagc tcgtcattca tatccgggtc aagagaagta
    11041 ccgataacca cagtcgcgtt gtcggaagca aatgcacgga tggtgttacc taccgtttcg
    11101 aactcatcca gacgcaggtc gaagcccgcc gtgatgttaa ccagcacgcc gcgcgcgcca
    11161 gacaggtcga tatcttccag cagcggagaa gagatagcca tttcagcagc ttcttccgca
    11221 cggtcttcac cgctcgccac gccagaaccc atcattgcgt agcccatctc agacattacg
    11281 gtgcgtacgt ctgcaaagtc cacgttcatc aaacccggac gagtaatcag ttcagcgata
    11341 ccttgcacag cgcctttcag tacatcgttc gctgcgccaa acgcatccag cagggagata
    11401 ccgcggccca gaactttcag cagtttgtcg ttcgggatag tgatcagaga gtccacatgc
    11461 ttggacagtt cagtgatccc ctgctccgcg aatgccatac gcttcttgcc ttcaaagttg
    11521 aaaggcttag tgacgacagc aacggtcagg atacccaaat cttttgccac ttcagcgacg
    11581 actggtgctg cacctgtacc ggtaccacca cccatacccg cagcaataaa gaccatgtct
    11641 gcaccttcca gcgccgcacg caatgcatcg cgatcctcat cagccgcatt gcggccaact
    11701 tctggattag cgccagcgcc cagtcctttg gtgataccgc taccgatttg aatcgtctgt
    11761 ccaaccgctg ttttacgcag cgcttgtgca tcggtattta ccgcgaagaa ttcaacacct
    11821 tcaatgcgct cgcgcaccat gtgttcaaca gcattaccgc cgccgccgcc gacgccgatg
    11881 actttaatca ccgcgtcatt ggtaagttcc attggttcaa acatagtttc tctccgattt
    11941 gtgcctgtcg cctgaggccg taatcatcgt cggcctcata aaaattaaaa ctcttttcgc
    12001 agccaactat tgagtcgctt gatccacgag ccaactgatg ctgtaacacg tttttctact
    12061 tcagcttcac cgttaagatg tgactctttc ccatagtgaa gcaatcccac cgccgtcgaa
    12121 taatacggct cctgagcata atccgttaaa ccggtaatgt tcagcggcgc gccgatacgc
    12181 acttgcgtat gaaacacgcg ctgagcacag gctgcaagac cttcgatctg cgctgcgcca
    12241 ccggttaata caatgcctgc cgccaggtga tgtttaaccc cttgttggcg aagcttttcc
    12301 tgcaactgca atatctcttc gttgaccagg ttgagcagct cggtatagcg cggctcgatc
    12361 acctctgcca gggtctgacg ttgcagactc cgtggcggac gaccgcctac gctcggcact
    12421 tccacgctct catcttttcc aacgatggaa cccagcgcac aaccgtggcg aactttaatc
    12481 gcttcggcgt cgcttggcgg cgtgccaaag gcgtaagcga tatcactggt cacgacattg
    12541 ccagcataag gaattacctt agtgtggcgc aatgccccac cggtataaac ggcgatatcc
    12601 attgtaccac ctccgatatc gacgacgcag acacccagtt cacgttcatc ttccgtcaat
    12661 accgaataac ttgatgccag tccggcaaat atcagttggt caactttcag cccacaacgt
    12721 tcaaccgctt tgacgatgtt tttcgccata tcgttgtgac atgtgatcag gtgcactttt
    12781 gcctgcatcc gcacgcccga aagtcctacc ggattcttga tcccttcctg atagtcaatc
    12841 gcatactctt gcgggatcac atgcagcaca cgatgttcat cgcgcacacg caccgatttc
    12901 gcggtatgga cgacgttttc cacatcttct tgcgtcactt cttcttcaga aataggcacc
    12961 ataccaattt cattctggca gctgatgtgc ttaccagaaa gcgccagata taccgaagag
    13021 atctgacaat ctgccatcaa ttctgcctgg tcaatggcgc gttgtacgca cttgaccacg
    13081 gattcgaggt cgttcacccc gcctttatcc ataccacgtg acgggcagct gcccacgcca
    13141 atgatattga ccataccgtc gggcagaact tcccctacta aagcggcaac cttcgcggta
    13201 ccaatctcca gtcctactac cagttttctg tccgtcgcct tgatcattgt tgttctgcct
    13261 gtgcctgatt ttgttgctga gtagattcct ctggcggcaa gggcgcccag cctactgccg
    13321 ctccagagtc ataacgcaaa tcaacgtagc taatccgttt gccatcggtt tgcgcctgct
    13381 gctgtaaaac cggataaagt tctacaaagc gagccaaacg tttcatcgta tcgccccggc
    13441 caagattgag cttaatatcg ttattcagcg tcaactgcca ggaacgccgc gcggtcatcg
    13501 ccgcttcctt cagagtaaat ctgtcctttg ccagcatctg ccccatttcg cgatagccct
    13561 gcaacacttc attggcgctg ccttccgggc catacagcat tggaagcacc tgcttgctgg
    13621 tgcgatctgg cggcacgctg aaggtgtttc cttccgcgtc taccatatgt tgatcattcc
    13681 accgcgcaat cggcacatat tcaaccagat gaatcttcaa ttcatcaggc cactgctttc
    13741 tgacgctcac ctgcttaatc cacggcaggc gttgttctat ttgcgtctgg atgatgttga
    13801 catcctgggt cataaaggta cccggctcac ccaatgccag gatcgactgc cggatatcgt
    13861 cattacgcgt gtaatggcgt tcaccggtca acaccagctt tgagagcggc aggcgttgcg
    13921 catcttccat ccagcccaac acgacccagc cgctcaccaa cactgtcgtt aaaacggtca
    13981 gcaggaaaag gatccccgcc agacgcgttc cattattgcg gcgagaagaa acctcttctt
    14041 cgctgtttcg cgtgttcaga gcagcctgcg acatattagt ccgccagttc cagaattcgt
    14101 actaccaact gcgagaagct catacctgcc tgacgtgccg ccatcggcac caggctgtgg
    14161 ctggtcatac ccggtgaggt attggcttcc agcagataaa actgtccatc gctgtccagc
    14221 ataacgtcaa tacgtcccca tcctttgcaa cctaacgtcg tccatgcttt cagcactaat
    14281 gcctgcaaat tggcctcttg tgacgcatcc agacctgcgg ggcagaaata ctgtgtctca
    14341 tcagagagat acttcgcctc ataatcatag aaggttccgg acggttgaat acgtattgac
    14401 ggtaaaattt cttcaccgag tatcgcaacc gtgaactccg gcccacttag ccatttttca
    14461 atcaatactt cttcatcgtg ctgaaatgcc aatcttaatg catcttgtag agcattttct
    14521 gctactactt ttgacattcc cacactggaa ccttcgcggc tcggcttaac gataaccggc
    14581 aaacccagag cagaaatttc tgctaactgc ttatcgctca ggcctttttc aaactctgcg
    14641 cgggttaacg ctacccacgg cgcgaccggt aaaccggcac cttgccacag cagtttgctg
    14701 cgtagtttat ccattgaaag cgcagatgcc atcactccgc tgccagtata aggcaagccc
    14761 atcagctcga gcatgccctg cagcgtacca tcttcaccgc cgcgaccgtg tagcgcgata
    14821 aacactttct gaaaacccat cgacttcagt tgcgtcacgt cgacttcttt cgggtcgaca
    14881 ggatacgcat caataccacc ttcacgcagc ccggctaaca ccgctgcgcc agaattcaga
    14941 gaaacttccc gctcagcgga agtcccgccc aacaggaccg cgattttatc agtcatgttg
    15001 ttcttcctcc ggagtttgcg gcttcagttt gatttcagct aaagaacggg caatttttcc
    15061 gatattacca gccccctgaa cgagaatcag gtcgttaccg gttaataccg gtgccagcat
    15121 ctcggctacc tgcgcagggt ccggcaccag aatgggatca attttcccac gtccacgaat
    15181 tgtgcgacac agcgaacggc tgtccgctcc cggaattggc gcttcgccag ccggatacac
    15241 ttccagcatc aacagggtat caacctgcgt cagcacattg gcgaaatcat catacaggtc
    15301 gcgcgtacgg gtaaaacggt gcggctgaaa cagcattacc aggtttttat ccggccagcc
    15361 tgcgcgcgcc gctttaatgg tggcgtcaac ttccgtcggg tggtggccgt agtcatcgac
    15421 cagcattgcc gtaccgcttt taccattcac tggctccagc gggaattcac cgaggaaatc
    15481 aaaacggcga ccagtcccca ggaagctctc cagcgcacgc aaaatagcct cgtcgtcaat
    15541 tccctcttcc gtagcaaccg caaccgcagc tgcggcgttc agcgcgttat gacggcctgg
    15601 cgcattcagg gtgacgcgca tcggctcttt gtcctggcgc agcagcgtaa agtgcccctg
    15661 cgggccaatc tgctgataat cttctacacg cacgtcggca tcttcgctga agccgtaagt
    15721 cgtggtctga cgtcccacac gcggtaacaa ttcgcggatc accggatcat caacacacat
    15781 caccgcacga ccgtaaaacg gcaggttgtg cagaaaatta ataaaagtct gttttaaatt
    15841 ctcaaagtcg ccctggtagg tatccatgtg gtcggcttcg atattggtga caatcgccac
    15901 catcggttgc agatgcagga acgatgcatc actctcatct gcttcggcaa tcaggtaccg
    15961 accatgcccc aaacgcgcat gaacccccgc cgcttttacc agcccgccgt taacgaaggt
    16021 tgggtcgagc cccgcttctg cgtagatgct ggaaaccatc gcggtggttg tcgttttgcc
    16081 gtgcgttcct gcaatggcga tgccatgacg aaaacgcatt aactcagcca gcatttcggc
    16141 acgacggatc accggaatac gcgcttcatg agctgcgaca atttccgggt tatcggcaga
    16201 aatggcgctg gaaacaacga ccacgctggc atcacgtacg ttttccgggc gatggttgaa
    16261 ataaatcgtc gctcccagat tcattaactg ctgcgtgacc ggatttggcg ctaaatcgga
    16321 accactgatc tgataacctt cattggccag aacttcggca ataccgccca taccggcacc
    16381 accgatgccg acaaaatgta tgtgccgaac gcgacgcatt tcgggcacga tggtacgcag
    16441 ttttgccaat tgttgtgtat tcattcttta cgccattaac ttcttaaatt catacgatgc
    16501 aaaaggcatc gctacaatta cgcccgggca gcccggctca cttcatttgc cactcgctcg
    16561 gtggcatccg gaatggatgc agcgcgggcg cgttctgcca tggttaataa ggtttctcgc
    16621 gaccacccgg ccagggtgtt ggcgacagca tccacgctaa gctgtggctg ctcgataatt
    16681 ttggctgcgc ccgctttttc cagcggtagc gcattccagt attgctggcg gtctttatgt
    16741 tgaaacggca caaacaacgc cggtagtcct gccgcggcga tttcactcac cgttaaagca
    16801 ccggagcggc aaacgacgac atccgcccac gcatacgccg ccgccatatc atcaataaat
    16861 tccgtcactt tatgctgcgg ttgccccgct tcggcatacg cctgttcaac ggattgttgc
    16921 gaacctttac cgctctgatg ccagatagtg actgaatcac ccagtttcgc agcaacctgc
    16981 ggcattgtct ggttaagaat gcgtgcgccc tgggaaccac ccactaccag cacacgaacc
    17041 ggaccttcac gtccagccaa acgttgctgc ggcaacggca gcgccagcac atcggtacgc
    17101 accgggttac ccaccacttc cgcattaggg aaagcgcctg gaaacgcctg catcactttg
    17161 gtggcaatct tcgccagcca tttattggtt aagcccgcaa taccgttttg ttcatgaagt
    17221 acaaccggaa tgcctaacga ccacgcggcc agaccacctg gacctgatac atagccgccc
    17281 ataccgagta ccacgtcagg tttgtacgct ttcataatcg cccgcgcctg acgccaggcg
    17341 ttgaagatac gcagcggcgc agctatcagt gcttttatac cttttccacg cagaccagag
    17401 atacgaatga aatcaatttc gatgccatgt tttggcacta agtccgcttc catacggtcg
    17461 gcagtcccca gccagcgaac ttgccaaccc tgagccatta gatggtgcgc aaccgccagt
    17521 cccgggaata catgtccacc ggttccgcct gccatcacca ttaatcgctt tccttgacca
    17581 ctcatcgtga acctcgtaca aacgcctgcg ctttctccag acgcgtttca taatcaatac
    17641 gcaacagcat catgatggct gtcgacataa tcagtaagct cgaaccaccg taactgatca
    17701 gcggcaatgt cagacctttg gtcggtaaca tccccgccgc cgcgcctacg ttaaccagcg
    17761 cctggaagct aaaccagatg ccaatagaac aggcgagaaa accggaaaaa cggtggtcaa
    17821 tttctaacgc tttgcggcca atcgacatcg cgcgaaaagc gacgaagaat accattaaaa
    17881 gtgccagcac cacaccgaca taccccagtt cttcgccgat aatggcgaaa ataaagtcag
    17941 tgtgcgcttc cggcagatac tccagttttt gtaccgagtt acctaaacct tgtccccaaa
    18001 gttcgccgcg accaaacgcc atcagcgatt gcgttaactg atagccgctg ccaaagggat
    18061 cttcccacgg gttccagaat gcggtaacac ggcggatacg gtacggttcg gcgagtatca
    18121 gcaacacaac cgctgaaatg cccataccga taatggcaat gaactgccac aatttcgctc
    18181 ccgccaggaa caacatcgcc agcgtagtca caaacaccac caccaccgta ccaaggtctg
    18241 gctgtgccag cagtaacact gccaacacca gaatcacgcc catcggtttc aggaagccgc
    18301 gcaggttatt acgtacttcg tcgcctttac gcaccagata gttggcgata taacaaaaca
    18361 gcgacagttt tgtcagctcc gcaggctgga tacgcagcaa accgagatcg atccaacgcg
    18421 atgccccttt aaccgagcta cccactacca gcacgatcat cagcaggatg atagatccga
    18481 gcagcatcgt ggcactgtag cgttgccaga actccatcgg cagacgcagc gtaatgatcg
    18541 ccagaatgaa tgccaggatc agatagacgc catcacgctt cgcgaagaag aacggatcgt
    18601 tggttaagcg ttgccctatg ggcattgacg ccgaggtcac catgataaag ccaatcgccg
    18661 caaggccgaa ggtcagccac agtaaggtgc gatcgtacat gatcaggctg tcggtatctt
    18721 tttcccgcga gcccatcacc cagcctttta gcgccgtgga gatccagacc aggatactga
    18781 atcctggcag gcgcggcatt ttcaggcgag ggagagataa acgcatcaac ctaactcctt
    18841 cgccagacgg gcaaactcat tgcctcgttg ttcaaagttc ttgaactgat caaggctggc
    18901 acaggccggg gagagcagaa ccatatcgcc cggctgaaca cgcggagcca gcaagcgcat
    18961 cgcctgttcc atagtttcgg tttgttctgc cacttccggg cgtagcgccg ccagctgcgc
    19021 accgtcacga ccaaaacagt acaaacgcac gttgtcgcca ttcagatagc gcgccagtgg
    19081 actgaagtcc gccgatttac catcgccgcc cagcaacaaa tgcagcgtgc cgtctacctg
    19141 caagccattc aacgccgctt ccgtactgcc gacgttagtg gctttcgaat cgttaatcca
    19201 gcgtacgcca ttgtgctcca gcaccacttc aaagcgatgc ggcagaccgg tgaaggtggt
    19261 taacgctttc aggctgctgg cgcgcggcaa accagctgcg tctgccagtg ccagtgctgc
    19321 cagcgcattg gtgtagttat gctgcccgga aagtttcatc tctttcacgt tcagcacttt
    19381 ctcgcctttc actcgcagcc aggtttctcc ctgctgatga ttcaggtgat agtcacccat
    19441 gttgacgcca aagctgacgc agcgttcatc agcaccgcga atcggcattg ttaaggcatc
    19501 atcagcattg acgacacaaa ctttcgcatt ttcgtaaata cgcaattttg ccgcacggta
    19561 ttgctgcaaa ccaaacggat agcggtccat atgatcttca gtcacgttca gaatggtcgc
    19621 cgccaccgcc tgtaagctgg aagtggtttc cagctggaag ctcgacagtt ccagcacgta
    19681 cagttcgcac tcagcatcaa gcagcatcaa cgctggcagg ccaatattgc cacccacacc
    19741 aacgttaacc cccgccgctt tcgccatttc acccactagc gtggtgaccg tgcttttgcc
    19801 gttagaaccg gtaatcgcca caatcggtgc ttgtgcttcg cggcagaaca gctcgatatc
    19861 gccaacgatt tcgattccgg catcagcggc agcgcttaag gatggatgcg ccagtgcaat
    19921 accgggactg gcgacaatca gatctgccgc catcagccat tcatcattca gactgcccgt
    19981 gtggcgttct acggcttcgg gtaatttatc caggctaggc ggtgtcatac gcgtatccat
    20041 aacgcgcggc gtcacaccgc gagcgaggaa aaagtccacg caggaaagcc cggtgaggcc
    20101 caggccgata atgacgacat ttttaccctg ataatcagcc atgattaacg taccttcagc
    20161 gttgccagac caatcagaac cagcatcagc gaaataatcc agaaacgcac aatgacgcgc
    20221 ggttccggcc agcctttcag ttcatagtgg tgatgaatcg gtgccatgcg gaaaatacgt
    20281 tgtccgcgca gtttaaagga gccgacctgc aggatgacag aaagcgtttc taccacgaac
    20341 acgcccccca taatcaccag caggaattcc tgacgtagca gtacggcgat aatgcctaac
    20401 gcaccaccta acgccagcga acctacatcg cccataaaga cctgcgccgg ataggtgtta
    20461 aaccacagga agcccagtcc tgccccgact atcgcggtac agacaataac cagttccccg
    20521 gcgtgtcgca gatacggtat gtgtaaatag ctggcaaagt tcatgttgcc ggtcgcccac
    20581 gccaccagcg caaaaccacc ggcgacaaat acggtcggca taattgccag gccgtcgaga
    20641 ccatcggtca ggtttaccgc gttgccagta cccacaatga cgaagtaagc cagcagaatg
    20701 tagaacagcc ccaactgcgg catcacatct ttaaagaatg ggaccaccag ctgcgttgcg
    20761 ggcgtgtctt tgccggcaag gtacagggcg aaggcgacac ccagcgcaat gaccgacatc
    20821 cagaaatact tccaacgagc gatcaaccct ttggtgtctt tacgcaccac tttgcgataa
    20881 tcatcaacaa agccaataac accgtaacct accagcacca ccaacacgca ccagacgtac
    20941 ggattggacg ggtaagccca cagcagtacg gagatcacaa tcgccgtcag gatcataatc
    21001 ccgcccatgg tcggcgtacc gcgcttgctg aagtgtgatt caggaccgtc gttacgcacc
    21061 acctgaccaa aggaaagttt ttgcaaatga gcaatcatac gcgggcccat ccacaatgag
    21121 atgaacagcg cggtcagcag gctgacgatg gcgcgaaacg tcagatagga aaagacgtta
    21181 aagccggaat aatatttgac caaatgttcg gccagccaaa ctaacatgtc ccattctcct
    21241 gtaaagcgcg tactacctct tccatggcgg cactacgtga acccttaact aaaatcgtaa
    21301 ttacctgttg ctcagcaatc agtgatttaa gacgcgtaat taacgcagtt ttatcagcaa
    21361 aatgttcgcc aacgccgctg gcggtgctga tagcatggct ttgtttaccc acgcttaaca
    21421 cgcggtcaat accagcagct tttgccgcct cgcccacctg tacatggcag gcttcgcttt
    21481 cagcgcccag ttccgccata tcgcccacca ccagcacgcg gtagcccggc atttcagcca
    21541 gtacctgaac agcggcggtc attgaaccga cattggcgtt gtaggagtcg tcgagcagca
    21601 actggttttc tgccagttgg atggggaaca gacggcctgg aacagctttc agatttgcca
    21661 gccccgcttt gatagcatca agcgttgcgc ccacggacat ggagagcgca gcggctgcca
    21721 gcgcattcgc aatattgtga cgccccggca acggcagcag aacatcgacg ctaccggttg
    21781 gggtttgtag ggtaaattcc gtaccgtgcg aggtcacatg gatattggtg gcggtgaaat
    21841 cgctgttggc ggcattgggt gagaaacgcc acactttgcg tgagccaatt acgctctgcc
    21901 agttcagcca gtcgttgttg tcggcgttca taatggcgat accgttttcc ggcaggccgc
    21961 taaagatttc acctttcgct ttcgcgacac ccgcaagcga gccaaaacct tccagatgcg
    22021 ccgctgccag gttgttgacc agcgcagctt ccgggcgagt cagactcaca gtccaggcta
    22081 tttcgccctg atggttcgcg ccaagttcaa taactgcgta atcgtattcc ggcgttaagc
    22141 gcaacaacgt catcggtaca ccgatgtcgt tgttgagatt gcctgccgta taaagcgtgt
    22201 tgccgcactg gcttaaaatc gccgccgtca tctctttaac ggaggttttg ccggaggaac
    22261 ctgtcagagc aaccacgcgc gccggaactt gctggcgaac ccatgcagcc agttcaccaa
    22321 acgccagacg cgtatccttg acgattaact gcggcaggtc gatatccagc ggacggctga
    22381 ccagtagtgc gcccgcgccg ccagctttcg cctggtcggc aaaatcgtgg gcatcaaaac
    22441 gttcgccttt cagggcaaca aacaggcagc ccggcgtcag ttttcgggta tcagtggtta
    22501 cagcatcaag ggtgatatct gcaccttgca gttcaccgtt gagaatgtcg gtaagttggc
    22561 taagggttac gctaatcatg caatcacccc cagcagacgc gccaccgtga cgcgatcgga
    22621 gtagtccaga cgctgattgc caacaatctg gtaatcttca tggcctttgc ccgcgaccag
    22681 caccacatcg ttctctttag cctgcataac ggcgcaagtc accgcttcag cacggccttc
    22741 catcactttg gcatgtccgg catctaacat tcccgccaga atatcgttga tgatggcacg
    22801 cggttcttcg gtacgcgggt tatcgtccgt caccaccgcc acgtcagcaa actcttcggc
    22861 aattgcgccc atcagtggac gcttaccttt atcgcgatcg ccaccacagc caaagacaca
    22921 ccacagcttg cccgcacagt gcagacgcgc cgcctgtaag gctttttcca gtgcatccgg
    22981 cgtatgcgcg taatccacca ccaccgtcgg tttgcctggc gcagtgaaca cttccatacg
    23041 tccgcaaacc ggttgcagac gcgcggcggt tttcaacaga tcagccaatg gatagccgag
    23101 tgccaacagt gtcgccagcg cgagcagcag gttgctgacg ttaaaagcgc ctatcagatg
    23161 gctttcaatt tcgccatcgc cccaacttga gctaaagcga atcgtcacac cgctgtcgtg
    23221 atagttcacg tcgatcactt ttaaccagcg tccgtgacag ttcagattaa tatgatcttc
    23281 cattgatacc gcaaccgcgt ccggcagctt tgccagccaa cggcggccca cttcatcgtc
    23341 ggcgttaata atcgcctgac cgcaatgatg ttcagaataa agcagccatt tcgcagcttc
    23401 gtagtgttcc atatcaccat gataatcaag gtgatcgcgg cttaagttgg taaagaccga
    23461 cgccgcaaat ttcaatgccg ccacacggta ctgtaccagc ccgtgggagg aaacttccat
    23521 tgcgcaaaac gtcgcgccct gctccaccag ccccgccagc tcatgctgaa catcgactgc
    23581 cgaaccggtg gtattttctg tcgggatcac tttccccagc agaccgttac caacggtgcc
    23641 cattaccgcg ctggtttcgc caagcagttg gctccactgc gccagcagct gggtagtcgt
    23701 ggttttgccg ttggtgcccg ttacgcctac gagacgtaaa ttgtcagagg gttcatggta
    23761 aaagcggccc gccagtgcag ataaacgctc gttgagctgg ctgagataga tgaccggtac
    23821 gccgtgcatt tcacggattt caccgtcggt cgcctcatct ttcgcctctg caataatggc
    23881 agccacacct tgcgctatcg cctgcgggat atatcgacgc ccgtccgcct gatgacctac
    23941 tacagctaca aagagatcgc ccgccgcagc cacacggctg tcgagtgtca tctctcgcag
    24001 tgctcgcgaa ggtgcgtctg gcacccacgg agcaagaagg tcgcgcaaat tacgatctgc
    24061 cacctgtccc ctcgccttga ttaatcacaa attcattttt atcgcccgtt gtcagcgcat
    24121 ccggctcgat gttcatggta cgcaatacgc cgcccatgat ggcaccaaag accggcgcgg
    24181 aaacggcgcc gccgtagtat ttacccgcct gcggatcgtt gataacaaca accagcgcga
    24241 agcgcggctg actcgcaggc gcaacgcctg cggtataagc aatatattta ttgatgtagc
    24301 gaccgtccga cccgaccttt ttcgcggtac cggttttaat ggcgatacga tagcctttaa
    24361 tcgccgcctt cacgccgccg ccgcccggta gcgccacgct ttccatcata tgcaccacgg
    24421 tgcggacaat ggattccggg aagacacgtt caccgggaac cggggggtca actttggtaa
    24481 tcgacagtgg gcgataaatg ccgtagctgc cgatagttgc gtagactcgc gctaactgta
    24541 acggtgttac cattagcccg tagccgaaag agaaggtggc cctctctatg tcagaccacc
    24601 gttgtttttg aggatataag ccactgcgtt ctccgaccaa ccccaaattg gtcgcttttc
    24661 ccagtccaaa acgtgagtaa gtatctacta acgctgagga cggcatcgct aacgccagct
    24721 tggaaacacc gacgttactc gacttctgta ataccccggt cagggttaat tcgctgtagc
    24781 gtgccacgtc tttgatttcg tggccgttaa ttcgataagg aatggtattg agtaccgagt
    24841 tttcccgcac cacgccacgt tgcaacgcgg tcatcaccac catcggttta accgttgagc
    24901 ccggttcaaa cacgtcggtg atggtacggt tacgcatcgc ctctttcggc gtgccgctca
    24961 gattgttagg gttgtatgac gggctgttag ccatcgccag cacttcacct gtgttgacat
    25021 ccaccagcac ggcgctacca gattcagcct tgttaaaggc caccgcgttg ttcagttcgc
    25081 gataaaccag cgcctgcagg cgttcatcaa tactcagcgc caggttgtgc gctgcctggc
    25141 tgtcagtaga agaaatatct tcaattacgc gaccatagcg gtctttacgc acaatgcgct
    25201 cacccggctg cccggtaagc catttatcga aactcttctc aacgccctca atcccttgac
    25261 tatcgacgtt agtaaagcca atgaggtgag cagtcacttc gccggatgga taataacggc
    25321 gagattcttc acgcagatga atacccggca gtttcagttt tttgatgtag tccgccatgt
    25381 cagggtttac ctgacgcgcc agataaataa agcgcccttt cgggttggcg ttaatgcggg
    25441 cagaaagctg atccagcgga atattgagcg cgttagccag cgccttccag cggtcaccga
    25501 cgctgatacc gccagcgtca tgcacttctt tcgggtcagc ccaaatcgct tttaccggca
    25561 cgctcaccgc taacgggcga ccagaacggt cagtaatcat gccgcgggag gtggaaactt
    25621 gctgaacgcg aagagaacgc atgtcgccct ctttcaccag catatccggg gagataactt
    25681 gtaaccacgc tacgcgtccg agcagaaaag ccagcgccag gagaatacag ccgcataaca
    25741 acgcaaaacg ccaactaata aagttggcat gttcttcctg acgttttggt ttctgcgttt
    25801 tcgccgctgc tttcatgcgt cgcgtttatc cttatttttg cactacgata ttttcttgtg
    25861 acggatcaac atgctgcatt tgcagctttt ccgtggcgat cctttccacc cggctatggt
    25921 cgccgagcgc attctcttca aggatcaggt tgcgccattc aatgtctaaa gcatctcgct
    25981 ccagcaccag ttgttcgcgc tgagcggtca gtaaacgggt atggtgcgcc gtagttacca
    26041 cggtcaccgc cgtcaaaata atgcaaatga acaggcagag tggcagcttc ccaaatcgca
    26101 aaagatcgtc accgataaca ccaggcaatg catggcgctc gtggcttccc atcgatcctt
    26161 taactttgct tagagcttct gtcactctgc tgatcatgcg ttcgtcctct ctgcgatacg
    26221 cagaactgaa ctacgggcac gaggattctc agccacctct tcttcgcccg gcattaactt
    26281 gcctaatgct cgcagctgac ggccacccag ttttttgagc tgctcttcag tcatcggtaa
    26341 ccctgccgga acttgcggac cgcggctgtt ttcacgcata aaacgtttca caatacggtc
    26401 ttccagcgag tggaagctga tgatcgaaag ccgcccaccc ggggccagca cgttgagcga
    26461 gctttttagc gcctgctcta tctcctccag ttcactgttt acccaaatgc gcaccgcctg
    26521 gaaggtacgg gtcgcgggat gtttaaactt atctttcacc ggcgttgcag cagccacgac
    26581 ttccgccagt tctttggtgc gggtcatcgg ctgttcgcgg ttacgctcga caatggcgca
    26641 ggcaatgcgt ttggcaaaac gctcttcacc ataggttttc aatacccagg cgatatcggc
    26701 ttcttctgcg gtttgtagcc attcagcggc tgactgccca cgggttgggt ccatacgcat
    26761 gtccagcgga ccatcgcgca taaaggaaaa gccacgttca gcatcatcaa gttgcggtga
    26821 agatacgcca agatcgagga gaatgccgtc gatcttgccg ataagatcgc gctcggcaac
    26881 gtattcgccc agcgcggaga aaggtccgtg gatgatggag aagcgcggat catcaatagt
    26941 cttcgcaacg gcgatagcct gcgggtcgcg atcgatcgcc agcaaacgcc cctcttcgcc
    27001 aagctgcgag aggatcagac gtgagtgacc accgcgacca aaagtcccat caatgtagat
    27061 gccatcagga cggatattga ggccattaac ggcttcatcc agcagcaccg tagtatgttt
    27121 atagttttcc atcattttat agagacaagt cctgcagtcg ctccgataag tttccagtag
    27181 ccaactgctc tgcgtcgata tcttccttga cctgttgatg ccaggttgtt tcatcccaca
    27241 gctcaaactt gttgaactgt ccaaccagca tcacttcttt tgtcagcccg gcatgttgcc
    27301 gcagtactgg cgcgattaac aatcgacctg cgccatccat ctgacattcg ctggcatgac
    27361 ctaacagtag gcgctgcaca cggcgctcaa ctgggttcat gctcgacaga cgcgataatt
    27421 tttgctcgat aacttcccat tcaggcaggg ggtaaagcag caggcacggg tgatgaatgt
    27481 caatggtgca aaccatttga ccggcagcgt tctcaagcag ctgttcccga taacgggtag
    27541 gcactgataa gcgccctttg ctgtcgagat tgactaacgt tgctccccgg aacatgccag
    27601 cctcacccct tattcccact ttgccccaca atttcccact gaaaggagtt tacggagcgg
    27661 aggaaaagct tgtcaagcca gtcacaaggc ataccgaggt aaaaaacccg atgtttacgg
    27721 ctaatatcag cgttgtttat tttacgtcca acgcgcgaag caaattaacg ttatgaatag
    27781 ctgtaagaaa aaatccctca tcgtcatcac tgttttaaaa tgactcacta ttttccacag
    27841 caaatataaa gtgtcagttt gcgacgcgag cggcatttta ggacatatct tccccggtta
    27901 acagtccttg ttacgtctgt gtggcgcttg atgccagcgg gtttgcgcaa aagcaaagaa
    27961 aagtgtttgt taaatcggca attcatcgcc gcatgtaaca aaataatgca aaaacgcgct
    28021 gtcgatatca cttaattatt gtgaatttca gctggtgagt taattaacgg gcgggtttaa
    28081 aaccatttat caataatagt caggaatagt cttatttact ttaagcatat tgatgtccag
    28141 tcccgtactc tacgcgccag agggaaattc acctggcgcg tatttttgtt cgcggcttag
    28201 ctacggctga gcacaccgcg gcgatagaga ttacgtttaa tgcgcgttaa accaggtttt
    28261 ggcttacgcg gttcgtctag gcttgccagg acaatctcca gcacacgctc tgcgacatcg
    28321 cggtgacgtt gagccactgc cagcaccggg cactgtaaga agtcgagcag ttcgttatcg
    28381 ccaaaggtgg caattgccag gtcagaaggc agtttaccat cgcgacgtag cgtgacatcc
    28441 atcacccctt gcaacaacgc aaacgacgtt gtgaacagcg cctgcggcat cggatgcgtt
    28501 tccagccatt tttcgaataa ctgggcagcc gcctcccgct catagctgtt ggcatacagg
    28561 aaatgcactt cgcgcggatc atctttccag gcagtacgga aaccttgttc acgcaggaag
    28621 ctgacagaaa gctccggcag cgcgccaaga taaagcaccg tctcggcggg aaacttacgt
    28681 aactcttccg ccagcatttc ggcatcatcc tgatcggcac caaccacgct ggtgaaatgt
    28741 tcacgatcga gggcgcggtc cagcgcgaca atcgggaacg ggtcgttagc ccagcgttga
    28801 taaaaaggat gctcaggtgg caacgacgtc gaaacaataa tggcatcaac ctgacgctgt
    28861 aaaaggtgct caatgcagcg catttcgttg tctggctgat cttctgagca ggcaatcagc
    28921 agttgataac cccgttgccg cgcctggcgc tcaagatagt tagcgatgcg ggtatagctg
    28981 gtgttctcca gatcggggat cacaagacca atagaacgtg tgcgtccagc acgaagccca
    29041 gctgccacgg cgttcgggtg gtaattgtgc tcacgcacca cagccatgac tttttcaacg
    29101 gttttgtcgc tcacacggta ttgcttcgct ttgccgttaa taacatagct tgcagtggtc
    29161 cgcgacactc ccgccagccg agcgatttca tccagtttca caattgcccc ttgcgtaaaa
    29221 tgtaaaaacc atagccattg tactggtatg ggttaaattc attaacatct aagcgcagaa
    29281 aattaactgc ggcaactgct tttattccag gttcccactg atttcgcaaa aaaagcccaa
    29341 cgtgacatgt tgggctgcaa attgcacact gagatcattc tcaacgcatt attttatcgc
    29401 cgcgcgaaag tccgaccaca ccagagcgag caacttccac aattttcgcc acctcgcgaa
    29461 tcaatgctaa aaatgcatca agcttatcgc tggtgcctgc taattgaacg gtataaagcg
    29521 agggggtgac atcgataatt tgcccacgga atatttccgt attacgtttc acttcgtcac
    29581 gcccgtaacc gctggcctga attttcacca gcatgatttc ccgctcaaca tgcgcgccct
    29641 gccccaactc actcacgcgc aagacatcga ccagcttgtg taattgcttt tcgatctgct
    29701 caagtacttt ttcatcgccc acggtctgga tggtcatacg cgataatgtc ggatcgtcgg
    29761 ttggcgcaac ggtcaggctt tcaatgttgt agcctcgctg ggaaaaaagg ccaatcacgc
    29821 gggataacgc gcctgattca ttttcgagta agactgataa tatccggcgc ataatcaggt
    29881 tctctccgtt ttgcttaacc acatttcatc cattccgccc ccgcgaatct gcatcgggta
    29941 gacgtgctca ctgccatcga cggtaacatc aacaaacacc aggcgattat tgcgcacctg
    30001 ttccagcgcc tcgctaagtt tgctttccag ctcatgcgga tgagagatct ggatcccgac
    30061 gtgaccatag gcttccgcca gacggacgaa atcgggtagt gattgcatat aagattgtga
    30121 atgacggccg gaatagatca tgtcctgcca ctgtttcacc atccccagat agcggttatt
    30181 gagattcacc accagtacgg gcaactcgta ttgcaacgcg gtagacagtt cctgaatgtt
    30241 catctgaata ctgccgtcgc cagtgacgca aaccacggtt tcttctggca acgccatttt
    30301 gacgcccagt gccgcaggta aaccaaaacc catcgtgccg aggccaccgg aattgatcca
    30361 gcgacgcggt ttgtcgaatg gataataaag tgcagcaaac atctggtgct gcccgacatc
    30421 ggacgtcacg taagcgtctc ccttcgtcaa ccgccaaaga gtctcgatca ccgcctgcgg
    30481 tttaatcttt tcactgtgag tgtcatattt caggcactga cgagcgcgcc actgttcaat
    30541 ttgctgccac cagtcgcgga tctcatccag tggttgatgg gcggattctt gcgacaagag
    30601 ttcaagcatt tgttcgagga cctggcgagc atcccccaca atcgggatat ccgcagtcac
    30661 ggttttagaa atggaagtag gatcaatatc gatatgcaga acagtggcat ttgggcagta
    30721 ctttgccaga ttgttcgtcg ttcggtcatc aaatcgtacc ccgacggcga aaatcacatc
    30781 cgcgttatgc atcgtcatat tggcttcgta ggtaccgtgc attcccagca tgcccagtgc
    30841 ctgacgatgc gttgccggaa acgcccccag ccccatcaat gaggaaacaa cgggcagatt
    30901 caacgcctcc accgtttctt tcaactgctg atggcagccc gccatgattg ccccaccgcc
    30961 tacgtagaca accggttttt ttgccactac cagcgtttgc agagcacgct taatttgccc
    31021 tttatgtccg gtagtagtgg gattgtaaga acgcatactg accgactccg gccagacata
    31081 gggtaatttg ttcgccggat taagaatatc tttcggtaaa tcaacgacta ctggtccagg
    31141 acgaccactt gccgccagcc agaaagcctt tttcagcacc tgcggaatgt cttccgtttg
    31201 cttaaccaga aaactgtgtt taaccactgg tcgcgaaatc cccaccatgt cgcactcctg
    31261 aaaggcatcg taacctatca acgaggtcgc tacctgcccg gaaaggacaa ctaatggaat
    31321 ggaatccata taagcggtgg cgatgccggt aatcgcattg gtcgcccctg gacccgacgt
    31381 taccagcacg acgccgactt ccccggtcgc gcgcgccaga ccatcggcca tatgcactgc
    31441 cgcctgctca tgacgaacta aaacatgatc gataccaccc acggtatgca atgcatcgta
    31501 aatatcaagg actgcgcctc caggataacc gaatacttgt ttaacgccct gatcgataag
    31561 cgatcggacg accatctcgg ctccagacaa catctccatg gcctgcctca ctgtttgatg
    31621 gaaaaaatgt gtaaaacaca ttaaccgtcc gatcttgctt aagcaattgg cagccagaga
    31681 cataaacgca gggtaataag aataaacagg aggaaaggtg aaaagaaacg ataaaatcct
    31741 ccattacgaa ggattaaatt gataataaat cggctgaatc ccacaactta cagcaatttc
    31801 tctatttttt taattaatcc taaaatagag agcccacaca ttcagaataa aattctacat
    31861 tcaataaaaa atgcagaata aaccagaaat tcatgtctga cctattctgc attcagttag
    31921 cgtttgcaaa ttgagactaa ttgttcttcc atccactgat ggcctttatc gcgcccggca
    31981 gcttcatgcc atgagagata acaggttctg ctgttttgtt ttaacggcag cggtaatacc
    32041 tgtaattcta aggattcagc gaactcttca gccagccaac gcggcgcaat agcgaccaaa
    32101 tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata cgcgatactg
    32161 gcttgcttat ctaccgtgtc ataccaaggt tgactaaatg acgcgaaacg atcgagcgaa
    32221 accgccgcat gttgttcgtt ataaacatca tgtttcagta acgggccttt aattgttgga
    32281 tgatttttgc tggctaccag caccatttca tctttaaata atggcacgct ggtaaattca
    32341 ggacgatgga agtcttcata actaatcaca aactccgttt cctgataacg cagctgatgt
    32401 tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc aatctgctca
    32461 atgtgattat aaatctgcga ggtcagaatg ctgtctaacg ggctgcaaac acaaagatga
    32521 aatacacgtt cactactcgc gggttcaaaa cctgaaccag gcaattcatt ttgtactagt
    32581 tgcaatgcct ggcgaactga accaaaaagt tgaaatgcgc gagcagtcgg ttgaatacca
    32641 cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc aacagcgtta
    32701 ctgaccgcag gttgcgacat acccagaaca tgagcggcac gggtaatgtt ttgctcctgc
    32761 atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg tggcttgctt
    32821 aactccaccg tctctgaatg atctgtttgt acctctggca tacttaactc cactgtcaca
    32881 cttaactccc tttcccttat tggaatgcag agaaataatc ctgaagaata tgatttatca
    32941 tgcatataaa ataagaaaaa gcaaaatgag taaaattcgg aaaacataaa gatatgggtt
    33001 ctgataaaaa cagaatccat tgccgtggaa tgagtcattt accacagcat aataatccat
    33061 aatgtttttg ttgcgaaaac aatctaatca taactaccgc gaatactcaa tcatctacaa
    33121 aatggattaa atgtgcttat ttaataatta atttatatat ttaatgcatt aattattaac
    33181 attaatggtt caataatatt caccaaatca atatcaaaaa aaattcgcaa aacatataat
    33241 tcaatacaaa tcatcaggat aggttttgca acgcgtgcat tttgtcccct ttttcctcgt
    33301 tgattagatg caaaaattta ttctgaaata tgtcaaccta tgaaaagtgt cggtcgttaa
    33361 acagaaatta atatcgcttg ctttaaccac cgcagcacaa ttagctaatt ttacggatgc
    33421 agaactcgcg ctggcgggac gtttttattg cgtcagggtt gacatccgtt tttgtatcca
    33481 gtaactctaa aagcatatcg cattcatctg gagctgattt aatgactcac atcgttcgct
    33541 ttatcggtct actactacta aacgcatctt ctttgcgcgg tagacgagtg agcggcatcc
    33601 agcattaagc cagcacgcag tcaaacaaaa aacccgcgcc attgcgcggg tttttttatg
    33661 cccgaagcga ggcgctctaa aagagacaag gacccaaacc atgagccagc aagtcattat
    33721 tttcgatacc acattgcgcg acggtgaaca ggcgttacag gcaagcttga gtgtgaaaga
    33781 aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt gacgtgatgg aagtcggttt
    33841 ccccgtctct tcgccgggcg attttgaatc agtgcaaacc atcgcccgcc aggttaaaaa
    33901 cagccgcgta tgcgcgttag ctcgctgcgt ggagaaagat atcgacgtgg cggccgaatc
    33961 cctgaaagtc gccgaagcct tccgtattca tacctttatt gccacttcgc caatgcacat
    34021 cgccaccaag ctgcgcagca cgctggatga agtgatcgaa cgcgctatct atatggtgaa
    34081 acgcgcccgt aattacaccg atgatgttga attttcttgc gaagatgccg ggcgtacccc
    34141 cattgccgat ctggcgcgag tggtcgaagc ggcgattaat gccggtgcca ccaccatcaa
    34201 cattccggac accgtgggct acaccatgcc gtttgagttc gccggaatca tcagcggtct
    34261 gtatgaacgc gtacctaaca tcgacaaagc cattatttcc gtacataccc acgacgattt
    34321 aggcctggcg gtcggcaact cactggcggc ggtacatgcc ggtgcacgcc aggtggaagg
    34381 tgcaatgaac gggatcggcg agcgtgccgg taactgttcg ctggaagaag tcatcatggc
    34441 gattaaagtt cgtaaggata ttctcaacgt ccacaccgcc attaatcacc aggagatatg
    34501 gcgcaccagc cagttagtta gccagatttg taatatgccg atcccggcaa acaaagccat
    34561 tgttggcagc ggcgcattcg ctcactcctc cggtatccac caggatggcg tgctgaaaaa
    34621 ccgcgaaaac tacgaaatca tgacaccaga atctattggt ctgaaccaaa tccagctgaa
    34681 tctgacctct cgttcggggc gtgcggcggt gaaacatcgc atggatgaga tggggtataa
    34741 agaaagtgaa tataatttag acaatttgta cgatgctttc ctgaagctgg cggacaaaaa
    34801 aggtcaggtg tttgattacg atctggaggc gctggccttc atcggtaagc agcaagaaga
    34861 gccggagcat ttccgtctgg attacttcag cgtgcagtct ggctctaacg atatcgccac
    34921 cgccgccgtc aaactggcct gtggcgaaga agtcaaagca gaagccgcca acggtaacgg
    34981 tccggtcgat gccgtctatc aggcaattaa ccgcatcact gactataacg tcgaactggt
    35041 gaaatacagc ctgaccgcca aaggtcacgg taaagatgcg ctgggtcagg tggatgttgt
    35101 cgccaactac aacggtcgcc gcttccacgg cgtcggcctg gccaccgata ttgtcgagtc
    35161 ctccgccaaa gccatggtgc acgtactgaa caatatctgg cgtgccgcag aagtcgaaaa
    35221 agagttgcaa cgcaaagctc aacacaacga aaacaacaag gaaaccgtgt gatgtcgaag
    35281 aattaccata ttgccgtatt gccgggggac ggtattggtc cggaagtgat gacccaggcg
    35341 ctgaaagtgc tggatgccgt gcgcaaccgc tttgcgatgc gcatcaccac cagccattac
    35401 gatgtaggcg gcgcagccat tgataaccac gggcaaccac tgccgcctgc gacggttgaa
    35461 ggttgtgagc aagccgatgc cgtgctgttt ggctcggtag gcggtccgaa atgggaacat
    35521 ttaccaccag accagcaacc agaacgcggc gcgctgttgc ctttgcgtaa gcacttcaaa
    35581 ttattcagca acctgcgtcc ggcaaaactg tatcaggggc tggaagcatt ctgtccgctg
    35641 cgtgctgaca ttgccgctaa cggcttcgac atcctgtgcg tgcgcgaact gaccggcggc
    35701 atctatttcg gtcagccaaa aggccgcgaa ggtagcggac agtatgaaaa agcgtttgat
    35761 accgaggtgt atcaccgttt tgagatcgaa cgtatcgccc gcatcgcgtt tgaatctgcc
    35821 cgcaagcgtc gccacaaagt cacctcaatc gacaaagcca acgtgctgca atcctctatt
    35881 ttatggcggg agatcgttaa cgagatcgcc acggaatacc cggatgtcga actggcgcat
    35941 atgtacatcg acaacgccac catgcagctg attaaagatc catcacagtt tgacgtcctg
    36001 ctgtgctcca acctgtttgg cgacattctg tctgacgagt gcgcaatgat cactggctcg
    36061 atggggatgt tgccttccgc cagcctgaac gagcaaggtt ttggtctgta tgaaccggca
    36121 ggcggctcag caccagatat cacaggcaaa aacatcgcca acccgattgc gcaaattctg
    36181 tcgctggcac tgctgctgcg ctacagcctg gatgccgatg atgcggctta cgccattgaa
    36241 cgcgccatta accgcgcatt agaagaaggc attcgcaccg gggatttagc ccgtggcgct
    36301 gccgccgtta gtaccgatga aatgggcgat atcattgccc gctatgtggc agaaggggtg
    36361 taatcatggc taaaacgtta tacgaaaaat tattcgacgc tcacgtagtg tacgaagccg
    36421 aaaacgaaac cccgctgtta tatatcgacc gccacctggt gcatgaagtg acctcaccgc
    36481 aggcgttcga tggtctgcgc gcccacggtc gcccggtacg tcagccgggc aaaaccttcg
    36541 ccactatgga tcacaacgtc tctacccaga cgaaagacat taatgcctgt ggtgaaatgg
    36601 cgcgcatcca gatgcaggag ctgattaaaa actgcaaaga atttggcgtc gaactgtatg
    36661 acctgaatca cccgtatcag gggatcgtcc acgtaatggg gccggaacag ggcgtcacct
    36721 tgccggggat gaccattgtc tgcggcgact cgcataccgc cacccacggc gcgtttggcg
    36781 cactggcctt tggtatcggc acttccgaag ttgaacacgt actggcaacg caaaccctga
    36841 aacagggccg cgcaaaaacc atgaaaattg aagtccaggg caaagccgcg ccgggcatta
    36901 ccgcaaaaga tatcgtgctg gcaattatcg gtaaaaccgg tagcgcaggc ggcaccgggc
    36961 atgtggtgga gttttgcggc gaagcaatcc gtgatttaag catggaaggt cgtatgaccc
    37021 tgtgcaatat ggcaatcgaa atgggcgcaa aagccggtct ggttgcaccg gacgaaacca
    37081 cctttaacta tgtcaaaggc cgtttgcatg cgccgaaagg caaagatttc gacgacgccg
    37141 ttgcctactg gaaaaccctg caaaccgacg aagtcgcaac tttcgatacc gttgtcactc
    37201 tgcaagcaga agaaatttca ccgcaggtca cctggggcac caatccaggc caggtgattt
    37261 ccgtgaacga caatattccc gatccggctt cgtttgccga tccggttgaa cgcgcgtcgg
    37321 cagaaaaagc gctggcctat atggggctga aaccgggcat tccgctgacc gaagtggcta
    37381 tcgacaaagt gtttatcggt tcctgtacca actcgcgcat tgaagattta cgcgcggcag
    37441 cggagatcgc caaagggcga aaagtcgcgc caggcgtgca ggcactggtg gttcccggct
    37501 ctggcccggt aaaagcccag gcggaagcgg aaggtctgga taaaatcttt attgaagccg
    37561 gttttgaatg gcgcttgcct ggctgctcaa tgtgtctggc gatgaacaac gaccgtctga
    37621 atccgggcga acgttgtgca tccaccagca accgtaactt tgaaggccgc caggggcgcg
    37681 gcgggcgcac gcatctggtc agcccggcaa tggctgccgc tgctgctgtg accggacatt
    37741 tcgccgacat tcgcaacatt aaataaggag cagaccatgg cagagaaatt tatcaaacac
    37801 acaggcctgg tggttccgct ggatgccgcc aatgtcgata ccgatgcaat catcccgaaa
    37861 cagtttttgc agaaagtgac ccgtacgggt tttggcgcgc atctgtttaa cgactggcgt
    37921 tttctcgatg aaaaaggcca acagccaaac ccggacttcg tgctgaactt cccgcagtat
    37981 cagggcgctt ccattttgct ggcacgagaa aacttcggct gtggctcctc gcgtgagcac
    38041 gcgccctggg cattgaccga ctacggtttt aaagtggtga ttgcgccgag ttttgctgac
    38101 atcttctacg gcaatagctt taacaaccag ctgctgccgg tgaaattaag cgatgcagaa
    38161 gtggacgaac tgtttgcgct ggtgaaagcc aatccgggga tccatttcga cgtggatctg
    38221 gaagcgcaag aggtgaaagc gggagagaaa acctatcgct ttaccatcga tgccttccgc
    38281 cgccactgca tgatgaacgg tctggacagt attgggctta ccttgcagca cgacgacgcc
    38341 attgccgctt atgaagcaaa acaacctgcg tttatgaatt aatccccttg cccggtcaaa
    38401 tgaccgggct ttccgctatc gtccacgtca tcaaacgtct ttaacctttg cggttaaaaa
    38461 taatgcgaca acagaaataa ccgcaattac ccagtagaca gcaaagtgcc cccaactttg
    38521 tgcaattgct ccctgaataa cgccagccag aattaccccg gtagaaatac tgttagtaaa
    38581 taaggtggta gctgcccccg ctcttccagg cattaaatcc tgaaaccata gcatcccaat
    38641 acccgcaaca atgccgataa atacagcgtt aaaaagttgc agcgtcatca acgccatacg
    38701 gctatgaaag aaaatcaatc cggtgtaaaa cagtactcct gccgccactg ctatgaccat
    38761 cattcgccgc ttaccataac gtttgacata gtagccagcc agaatcattg ctggtatttc
    38821 cagtccagct gccgtcccca tcaggaaacc cgccagtttg tctggcaatc ctaactcgct
    38881 actgatccac aacggcatat caataatgta catggtgttg caggtccaca ttaacgtcga
    38941 ggcgacaaat aacatccgta cgttactatc ctgccagcca ccttgcattg ataaagcatt
    39001 ttccgacggc agttctaccc gcgccacaga cggaagcata aatgcaatca ataccagact
    39061 gagtgtgaat atcccggcgg caatcgaaaa catcaccact tctcgcgccg agttatccgc
    39121 atattcccgc gccagagcaa ataactgtgg cattgccgta ttggccagag atgccagaag
    39181 cacaccacag gtgataagcg tcagataatg acgattaaat gcaaacaatg gcgcattgcc
    39241 gatagccatc aaacagcaaa atataatcag ttttcgccga tcgccctgac tgtctgaacg
    39301 ttttgccaac cagaggctta cgccgatccc agcaatagta ttcaccgtat aaaagagtcc
    39361 gatccagaaa ggttgcgcgc caacctcacg actcagaaat aagctcaatg taggagcctg
    39421 tagcgccccg gcccccccta tcataaaagc gaccagcata aatgccgcgt aaacaccgtt
    39481 catacggcga gccatcgtca ttatccagat catacgttcc ctttttagcg cggcgagaat
    39541 aaaaaaaacc agcaggtata atctgctggc gggtgatttt acaccaatac tcagtcacac
    39601 atgatgcagg caagtcaact ttcagaattg cggtcatccc actgcatcag tccttcaagc
    39661 atggttaatc gttgtgggac gcttaaccaa cgcaaccagc acaacttcgc tgtcgcggta
    39721 aaatagtgct gataaaactg acgcatgggg cacctccttg cttcatcgtt aggaattatt
    39781 gacttaatat agggaaaata aagttgctgt cttttgcgca ggagttcccc ttttatgtca
    39841 tctgctcgtc tgcaacaaca gttcatccgc ctgtggcaat gctgcgaggg taaatcgcag
    39901 gacacaacgc tcaacgaact ggcagcgtta ttgagctgct cgcgtcgtca tatgcgtacc
    39961 ctgctcaaca ccatgcagga tcgcggctgg ctgacgtggg aagcggaagt cgggcgcggt
    40021 aaacgctcgc gtctgacatt cctctatacc gggctggcgc ttcagcaaca gcgggcggaa
    40081 gacctgctgg agcaggatcg tatcgaccaa ctagtgcagt tggttggcga caaagcgact
    40141 gtgcggcaaa tgctggtttc tcatctgggc cgcagcttcc gccaggggcg gcacatcctg
    40201 cgcgtgctct actatcgtcc gttgcgtaat ctgctacctg gcagcgcatt gcgccgttcc
    40261 gaaacccata ttgcccggca aatcttcagt tcgctaacgc gcataaatga ggaaaatggg
    40321 gaactggaag cagacatcgc ccaccactgg cagcaaatat caccgcttca ctggcgtttc
    40381 tttttgcgtc caggagtcca ttttcatcat ggtcgtgaac tggaaatgga cgacgtgatc
    40441 gcctctttaa aacgaatcaa tacgctgccg ctctattcgc atattgcgga cattgtgtcg
    40501 ccgacgccct ggacgctgga tatccatctc acgcaaccgg accgctggtt accgttactg
    40561 ctggggcaag ttccggcgat gatcctgccg cgcgaatggg aaaccctcag taactttgcc
    40621 agccatccca tcggcaccgg tccgtatgcg gtgatccgca acaccaccaa tcaactgaaa
    40681 attcaggcat tcgatgactt cttcggttac cgggcattaa tcgatgaagt taacgtctgg
    40741 gttctgccgg aaattgccga cgagccagct ggaggtctga tgctaaaagg tccacagggt
    40801 gaggaaaaag agattgaaag ccgcctggag gaaggttgct actatttact attcgacagc
    40861 cgcacccatc gcggagcgaa tcagcaagtc agggactggg taagctatgt gctttctcca
    40921 actaatctgg tctatttcgc tgaggaacag taccagcaac tgtggttccc ggcttatgga
    40981 ctgctccccc gttggcacca tgcccgcacc ataaagagcg aaaaaccggc tggcctggaa
    41041 agcctcaccc tgacctttta tcaggatcac agtgagcatc gggtgattgc cgggatcatg
    41101 cagcagattc tggcaagtca ccaggtcacg ctggaaatca aagagatcag ctacgatcag
    41161 tggcatgaag gagagatcga gagcgatatc tggcttaata gcgccaactt tacgctgccg
    41221 ctggattttt cgctgttcgc gcacttgtgc gaggtgccgc tgctccaaca ctgtattccg
    41281 atcgactggc aagccgacgc cgcccgctgg cgcaatggcg aaatgaacct ggcgaactgg
    41341 tgccagcaac tggtcgccag taaagcaatg gtgccgctta tccaccactg gctgatcatt
    41401 cagggacaac gcagtatgcg cggcctgcgt atgaataccc tcggctggtt cgattttaaa
    41461 tcagcgtggt ttgcgccgcc ggatccatga ttgctggtag ataacaaaat cactacacta
    41521 acgccgttct caacggggtg ccacgcaaac gcgtgcgctg agaaaatacc cgtcgaacct
    41581 gatccggata acgccggcga agggatttga ggctccttct caagtccttt gccactcttt
    41641 tttgaggtgc aaagtgttaa aaaaatgtct tcccctgctg ttgctgtgca cagcgcccgt
    41701 tttcgctaaa cccgttctga ctgtttatac ctacgattcc ttcgccgccg actgggggcc
    41761 tggtccaaag atcaaaaaag cttttgaagc cgactgtaac tgcgaactga aactggtggc
    41821 gctggaagat ggcgtttcgc ttctcaaccg tctacggatg gaaggcaaaa atagtaaagc
    41881 cgatgtggtg ctggggctgg ataacaatct gttagacgcc gccagtaaaa ccggactatt
    41941 tgccaaaagc ggtgtggcag cggatgccgt taacgttccc ggcggctgga ataatgacac
    42001 tttcgtccag tttgattacg gctatttcgc cttcgtctat gacaagaaca aactgaaaaa
    42061 cccgccgcaa agcctgaaag aattggttga gagcgatcaa aactggcggg tgatttatca
    42121 ggatccgcgc accagtacgc cggggctggg cctgctgttg tggatgcaaa aagtctatgg
    42181 cgatgacgcc ccacaagcct ggcaaaaact ggcgaagaaa accgtcacgg tcaccaaagg
    42241 ctggagcgaa gcctacggcc tgtttttaaa aggtgaaagc gatctggtac tgagttacac
    42301 cacctctccg gcttatcaca ttctcgaaga gaaaaaagat aactacgccg ccgcgaactt
    42361 tagcgaaggt cactatctgc aggtggaagt cgccgcccgc accgctgcca gcaagcagcc
    42421 ggagctggcg caaaaattcc tccagtttat ggtttctccg gcttttcaga atgcgatccc
    42481 aaccggtaac tggatgtatc cggtggcaaa tgtcacgctg cctgccgggt ttgaacaatt
    42541 gaccaaaccg gccaccacgc tggagttcac accagctgaa gtggcggcac aacgtcaggc
    42601 atggattagc ggatggcaac gcgccgtcag ccgttaattc ccggctggtt aattccaggt
    42661 gtaagcgccg ccacgctggt ggtagcggtt gcgctcgcgg cgtttctcgc cctgtggtgg
    42721 aacgcgccgc agggtaactg ggtggcagtc tggcaggaca gctacctgtg gcatgtggtg
    42781 cgcttctcct tctggcaggc gtttctctcg gcgctactct ctgtcgtacc cgcgatattc
    42841 ctcgcccgcg ccctctatcg caggcgcttt ccgggtcggc tggcgctgtt gcgtctgtgc
    42901 gcaatgacct tgatcctccc ggtgctggtt gctgttttcg gcattcttag cgtctatggt
    42961 cgccagggct ggctggcatc gctctgccaa tcgctcggtc tggagtggac cttttcgccc
    43021 tacggcctgc aaggtatttt gctggcgcac gtatttttta atctgccgat ggcgagccgc
    43081 ttattactcc aggcactgga aaacattccc gacgaacaac gtcagcttgc cgcccagctt
    43141 gggatgcgcg gctggcattt tttccgcttc gtcgaatggc cgtggttacg gcgacaaatc
    43201 ccgccggttg ctgcacttat ctttatgctc tgtttcgcca gcttcgccac cgtgctatca
    43261 ctgggcggcg gtccgcaggc gaccactatc gagctggcta tttatcaggc gctgagttac
    43321 gactacgatc ctgcccgcgc ggcgatgctg gcgctgatcc agatggtgtg ttgccttggg
    43381 ctggtactgt tgagtcagcg attgagtaag gccattgcgc caggcaccac gctgctgcaa
    43441 ggctggcgcg acccggacga tcgtctgcat agccgcattt gcgacacggt gttaatagtg
    43501 ctggcgctgc tgctgttgtt gccaccgttg ctggcggtga tcgtcgatgg ggtaaatcgc
    43561 cagttgccgg aagtgctggc acaaccggtg ctgtggcagg cgctgtggac ctcgttgcgt
    43621 attgcgctgg cggcaggtgt attgtgcgta gtgctgacca tgatgctgct atggagcagt
    43681 cgcgaacttc gggcgcggca gaaaatgctg gcgggtcagg cgctggagat gagcggcatg
    43741 ttgattctcg ccatgccggg gattgtgctg gctaccggct tctttttact gctcaacaac
    43801 actatcggcc tgcaacaatc tgctgacggc attgtgattt tcaccaatgc gttaatggcg
    43861 atcccttatg cgctgaaagt actggaaaac ccgatgcgcg atatcaccgc ccgctacagt
    43921 atgttgtgtc agtcgctggg cattgaaggc tggtcgcgct taaaagtggt ggagctgcgc
    43981 gccctgaaac gtccactggc gcaggcgctg gcctttgcct gcgtgctgtc gattggtgat
    44041 tttggcgtgg tggcgttgtt cggtaacgat gatttccgta ccctgccgtt ttatctctac
    44101 cagcaaattg gctcctatcg cagccaggac ggcgcggtca ccgcgttaat tctgctgcta
    44161 ctctgttttc tgctgtttac cgtgattgaa aaactaccgg ggcgaaatgt taaaactgac
    44221 tgatatcacc tggctttacc aacatttgcc gatgcgtttt agcttaacgg tggaacgcgg
    44281 cgagcaggtg gcgatcctcg ggccaagcgg cgcaggtaaa agtaccctgc tgaatttgat
    44341 tgccggtttt ctgacgccag ccagcggttc gctgactatc gatggcgttg atcacaccac
    44401 gacgccgccg tcacgccgtc cggtgtcgat gctgtttcag gagaacaacc tgttcagcca
    44461 cctgacggtc gcgcagaaca tcgggctggg gcttaatcca ggattgaagc tgaacgcggc
    44521 acagcaggag aaaatgcacg ctatcgcccg tcagatgggg attgataatt taatggcgcg
    44581 gttaccgggc gagctttccg gcggccagcg acagcgcgtg gcgttagcgc gttgtctggt
    44641 gcgcgaacag ccgattttat tgctcgatga accgttctct gcgctcgatc cggcgttacg
    44701 tcaggagatg ctgacgctgg tgagcacgag ctgccagcag caaaaaatga cgctattgat
    44761 ggtgtcgcac agtgtggaag atgcggcgcg gatcgccaca cgctcggtgg tggtcgccga
    44821 cgggcgcatc gcctggcagg gtaagactga agaattgttg agcggtaagg cgagtgcttc
    44881 ggcaatattg gggattacgg gttagtatgc actgtcagat gcggcgtgaa caccttatcc
    44941 gacctacgga cagtgcgttt tgtaggcctg ataagacgcg gtcagcgtcg catcaggcaa
    45001 cccgcacaag acaatcccct aaaccccaac cactttacgc aaaatatcga tatacaccgg
    45061 catcaacggg tggcgaatta acaccaccag cgccaccacg ccgatggcag aaatcaacgg
    45121 cgtcagccac aacaaacggc cgcgcgacaa ataatgactt aaacgatcgg ttgctttacc
    45181 gctgcgccat aaccgccagc acagccagcc accaacccac aaaaataccg ccgtcgccag
    45241 cagcaaccat ttaaactcgc cgctctgcat tccggctgga atatcgatcg ccgcgcccgc
    45301 cagaatccca ggcaggaagt aaaacggtgg ccacagcagg cagccgataa tattcggcgt
    45361 aatgaatttt gccaccggca gatccagcat tcccgccacc attggcacca gcggacgcgt
    45421 cgggccaaca aagcgaccga ccagaatggt gaacatgctg tgttgatgca acgcatgttc
    45481 agttttatcg agtaatgctt tgtttttctt cagaaatgac cagcgatgca acggcttttt
    45541 aaaacgccag cccagccaga aggagatcca gtcgcccagc agacaaccga caatccctgc
    45601 cagccaggcg tgccagaaac ttaactcgcc gctgccaatc agcgctccca gccccgccat
    45661 cagcaccgta ccgggtagaa tcaaaccgac cagcgccagc gactccagaa aggccaccaa
    45721 caccaccgcc atcaatgaat acacggtaga ttgggtaata aagtgttcca gcaatgcttg
    45781 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
    45841 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
    45901 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
    45961 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
    46021 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
    46081 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
    46141 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
    46201 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
    46261 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
    46321 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
    46381 ccgggcgaaa gaaacccgta ttgacaaata ttgacggcca gttaagccat tcatgccagt
    46441 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
    46501 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
    46561 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
    46621 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
    46681 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
    46741 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
    46801 gacattgccg tcactgcgtc ttttactggc tcttctcgct aacccaaccg gtaaccccgc
    46861 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
    46921 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
    46981 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
    47041 tctctactgt ttctccatac ccgttttttg gatggagtga aacgatggcg attgcaattg
    47101 gcctcgattt tggcagtgat tctgtgcgag ctttggcggt ggactgcgcc agcggtgaag
    47161 agatcgccac cagcgtagag tggtatcccc gttggcaaaa agggcaattt tgtgatgccc
    47221 cgaataacca gttccgtcat catccgcgtg actacattga gtcaatggaa gcggcactga
    47281 aaaccgtgct tgcagagctt agcctcgaac agcgcgcagc tgtggtcggg attggcgttg
    47341 acagtaccgg ctcgacgccc gcaccgattg atgccgacgg taacgtgctg gcgctgcgcc
    47401 cggagtttgc cgaaaaccca aacgcgatgt tcgtattgtg gaaagaccac actgcggttg
    47461 aagaagcgga agagattacc cgtttgtgcc acgcgccggg caacgttgac tactcccgct
    47521 acattggtgg tatttattcc agcgaatggt tctgggcaaa aatcctgcat gtgactcgcc
    47581 aggacagcgc cgtggcgcaa tctgccgcat cgtggattga gctgtgcgac tgggtgccag
    47641 ctctgctttc cggtaccacc cgcccgcagg atattcgtcg cggacgttgc agcgccgggc
    47701 ataaatctct gtggcacgaa agctggggcg gcttgccgcc agccagtttc tttgatgagc
    47761 tggacccgat cctcaatcgc catttgcctt ccccgctgtt cactgacacc tggactgccg
    47821 atattccggt gggcacctta tgcccggaat gggcgcagcg tctcggcctg cctgaaagcg
    47881 tggtgatttc cggcggcgcg tttgactgcc atatgggcgc agttggcgca ggcgcacagc
    47941 ctaacgcact ggtaaaagtt atcggtactt ccacctgcga cattctgatt gccgacaaac
    48001 agagcgttgg cgagcgggca gttaaaggta tttgcggtca ggttgatggc agcgtggtac
    48061 ctggatttat cggtctggaa gcaggccaat cggcgtttgg tgatatctac gcctggtttg
    48121 gtcgcgtact cggctggccg ctggaacagc ttgccgccca gcatccggaa ctgaaagcgc
    48181 aaatcaacgc cagccagaaa caactgcttc cggcgctgac cgaagcatgg gccaaaaatc
    48241 cgtctctgga tcacctgccg gtggtgctcg actggtttaa cggccgccgc acaccgaacg
    48301 ctaaccaacg cctgaaaggg gtgattaccg atcttaacct cgctaccgac gctccgctgc
    48361 tgtttggcgg tttgattgct gccaccgcct ttggcgcacg cgcaatcatg gagtgcttta
    48421 ccgatcaggg gatcgccgtc aataacgtga tggcgctggg cggcatcgcg cggaaaaacc
    48481 aggtcattat gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt gttgcctctg
    48541 accagtgctg tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa gtgcacgcag
    48601 acatcccatc agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg caaccgcgca
    48661 gcgaacaggc acaacgcttt gaacagcttt atcgccgcta tcagcaatgg gcgatgagcg
    48721 ccgaacaaca ctatcttcca acttccgccc cggcacaggc tgcccaggcc gttccgactc
    48781 tataaggaca cgataatgac gatttttgat aattatgacg tgtggtttgt cattggcagc
    48841 cagcatctgt atggcccgga aaccctgcgt caggtcaccc aacatgccga gcacgttgtt
    48901 aatgcgctga atacggaagc gaaactgccc tgcaaactgg tgttgaaacc gctgggcacc
    48961 acgccggatg aaatcaccgc tatttgccgc gacgcgaatt acgacgatcg ttgcgctggt
    49021 ctggtggtgt ggctgcacac cttctccccg gccaaaatgt ggatcaacgg cctgaccatg
    49081 ctcaacaaac cgttgctgca attccacacc cagtttaacg cggcgctgcc gtgggacagt
    49141 atcgatatgg actttatgaa cctgaaccag actgcacatg gcggtcgcga gttcggcttc
    49201 attggcgcgc gtatgcgtca gcaacatgcc gtggttaccg gtcactggca ggataaacaa
    49261 gcccatgagc gtatcggctc ctggatgcgt caggcggtct ctaaacagga tacccgtcat
    49321 ctgaaagtct gccgttttgg cgataacatg cgtgaagtag cggtcaccga tggtgataaa
    49381 gttgccgcac agatcaagtt cggtttctcc gtcaatacct gggcggttgg cgatctggtg
    49441 caggtggtga actccatcag cgacggcgat gttaacgcgc tggtcgatga gtacgaaagc
    49501 tgctacacca tgacgcctgc cacacaaatc cacggcgaaa aacgacagaa cgtgctggaa
    49561 gcggcgcgta ttgagctggg gatgaagcgt ttcctggaac aaggtggctt ccacgcgttc
    49621 accaccacct ttgaagattt gcacggtctg aaacagcttc caggtctggc cgtacagcgt
    49681 ctgatgcagc agggttacgg ctttgcgggc gaaggcgact ggaaaaccgc cgccctgctt
    49741 cgcatcatga aggtgatgtc aaccggtctg cagggcggca cctcctttat ggaggactac
    49801 acctatcact tcgagaaagg taatgacctg gtgctcggct cccatatgct ggaagtctgc
    49861 ccgtcgattg ccgtagaaga gaaaccgatc ctcgacgttc agcatctcgg tattggtggt
    49921 aaggacgatc ctgcccgact gatcttcaat acccaaaccg gtccagcgat tgtcgccagc
    49981 ctgattgatc tcggcgatcg ttaccgtctg ctggttaact gtatagacac ggtgaaaaca
    50041 ccgcactccc tgccgaaact gccggtggcg aatgcgctgt ggaaagcgca accggatctg
    50101 ccaactgctt ccgaagcgtg gatcctcgct ggtggcgcgc accataccgt cttcagccat
    50161 gcgctgaacc tcaacgatat gcgtcagttc gccgagatgc acgacattga aatcacggtg
    50221 attgataacg acacccgcct gccagcgttt aaagacgcac tgcgctggaa cgaagtgtat
    50281 tacggatttc gtcgctaagt agccacatca ggtatgtaac gcctgatgcg acgctgacgc
    50341 gtcttatctg gcctacacgc tgcgattttg taggccggat aagcaaagcg catccggcat
    50401 tcaacgcctg atgcgacgct ggcgcgtctt atcaggccta cgcgctgcga ttttgtagac
    50461 cggataagcg aagcgcatcc ggcacgaagg agtcaacatg ttagaagatc tcaaacgcca
    50521 ggtattagaa gccaacctgg cgctgccaaa acacaacctg gtcacgctca catggggcaa
    50581 cgtcagcgcc gttgatcgcg agcgcggcgt ctttgtgatc aaaccttccg gcgtcgatta
    50641 cagcgtcatg accgctgacg atatggtcgt ggttagcatc gaaaccggtg aagtggttga
    50701 aggtacgaaa aagccctcct ccgacacgcc aactcaccgg ctgctctatc aggcattccc
    50761 ctccattggc ggcattgtgc atacgcactc gcgccacgcc accatctggg cgcagtcggg
    50821 tcagtcgatt ccagcaaccg gcaccaccca cgccgactat ttctacggca ccattccctg
    50881 cacccgcaaa atgaccgacg cagaaatcaa cggcgaatat gagtgggaaa ccggtaacgt
    50941 catcgtagaa acctttgaaa aacagggtat cgatgcagcg caaatgcccg gcgttctggt
    51001 ccattcccac ggcccgtttg catggggcaa aaatgccgaa gatgcggtgc ataacgccat
    51061 cgtgctggaa gaggtcgctt atatggagat attctgccgt cagttagcgc cgcagttacc
    51121 ggatatgcag caaacgctgc tggataaaca ctatctgcgt aagcatggcg cgaaggcata
    51181 ttacgggcag taatgactgt ataaaaccac agccaatcaa acgaaaccag gctatactca
    51241 agcctggttt tttgatggat tttcagcgtg gcgcaggcag gttttatctt aacccgacac
    51301 tggcgggaca ccccgcaacg gacagaagtc tccttctggc tggcgacgga caacgggccg
    51361 ttgcaggtta cgcttgcacc gcaagagtcc gtggcgttta ttcccgccga tcaggttccc
    51421 cgcgctcagc atattttgca gggtgaacaa ggctttcgcc tgacaccgct ggcgttaaag
    51481 gattttcacc gccagccggt gtatggcctt tactgtcgcg cccatcgcca attgatgaat
    51541 tacgaaaagc gcctgcgtga aggtggcgtt accgtctacg aggccgatgt gcgcccgcca
    51601 gaacgctatc tgatggagcg gtttatcacc tcaccggtgt gggtcgaggg tgatatgcac
    51661 aatggcacta tcgttaatgc ccgtctgaaa ccgcatcccg actatcgtcc gccgctcaag
    51721 tgggtttcta tagatattga aaccacccgc cacggtgagc tgtactgcat cggcctggaa
    51781 ggctgcgggc agcgcatcgt ttatatgctg gggccggaga atggcgacgc ctcctcgctt
    51841 gatttcgaac tggaatacgt cgccagccgc ccgcagttgt tggaaaaact caacgcctgg
    51901 tttgccaact acgatcctga tgtgatcatc ggttggaacg tggtgcagtt cgatctgcga
    51961 atgctgcaaa aacatgccga gcgttaccgt cttccgctgc gtcttgggcg cgataatagc
    52021 gagctggagt ggcgcgagca cggctttaaa aacggcgtct tttttgccca ggctaaaggt
    52081 cggctaatta tcgacggtat cgaggcgctg aaatccgcgt tctggaattt ctcttcattc
    52141 tcgctggaaa ctgtcgctca ggagctatta ggcgaaggaa aatctatcga taacccgtgg
    52201 gatcgaatgg acgaaattga ccgccgtttc gccgaagata aacctgcgct ggcaacttat
    52261 aacctgaaaa attgcgagct ggtgacgcag atcttccaca aaactgaaat catgccattt
    52321 ttactcgaac gggcaacggt gaacggcctg ccggtggacc gacacggcgg ttcggtggcg
    52381 gcatttggtc atctctattt tccgcgaatg catcgcgctg gttatgtcgc gcctaatctc
    52441 ggcgaagtgc cgccgcacgc cagccctggc ggctacgtga tggattcacg gccagggctt
    52501 tatgattcag tgctggtgct ggactataaa agcctgtacc cgtcgatcat ccgcaccttt
    52561 ctgattgatc ccgtcgggct ggtggaaggc atggcgcagc ctgatccaga gcacagtacc
    52621 gaaggttttc tcgatgcctg gttctcgcga gaaaaacatt gcctgccgga gattgtgact
    52681 aacatctggc acgggcgcga tgaagccaaa cgccaaggta acaaaccgct gtcgcaggcg
    52741 ctgaaaatca tcatgaatgc cttttatggc gtgctcggca ccaccgcctg ccgcttcttc
    52801 gatccgcggc tggcatcgtc gatcaccatg cgtggtcatc agatcatgcg gcaaaccaaa
    52861 gcgttgattg aagcacaggg ctacgacgtt atctacggcg ataccgactc aacgtttgtc
    52921 tggctgaaag gcgcacattc ggaagaagaa gcggcgaaaa tcggtcgtgc actggtgcag
    52981 cacgttaacg cctggtgggc ggaaacgctg caaaaacaac ggctgaccag cgcattagaa
    53041 ctggagtatg aaacccattt ctgccgtttt ctgatgccaa ccattcgcgg agccgatacc
    53101 ggcagtaaaa agcgctacgc cgggctgatt caggagggcg ataaacagcg gatggtgttt
    53161 aaagggctgg aaaccgtgcg caccgactgg acgccgctgg cccagcagtt tcagcaggag
    53221 ctatacctgc gcatcttccg caacgagcca tatcaggaat atgtacgcga aaccatcgac
    53281 aaactgatgg cgggtgaact ggatgcgcga ctggtttacc gtaaacgcct tcgccgtccg
    53341 ctgagcgagt atcagcgtaa tgtgccgcct catgtacgcg ccgctcgcct tgccgatgaa
    53401 gaaaaccaaa agcgtggtcg ccccttgcaa tatcagaatc gcggcaccat taagtacgta
    53461 tggaccacca acggcccgga gccgctggac taccaacgtt caccactgga ttacgaacac
    53521 tatctgaccc gccagctaca acccgtggcg gagggaatac tcccttttat tgaggataat
    53581 tttgctacac ttatgaccgg gcaacttggg ctattttgag caaaaaaaga gttcgccaga
    53641 taccattttg atgcgtgacg aatgctttgc catccagtac catagcgccc tttccattcc
    53701 tggacctgaa taacaccact acctcataag cacggtagca ggtggttatt gcctgcaatt
    53761 aaagatatag agccgaacac atatgccttt tacacttggt caacgctgga tcagcgatac
    53821 agaaagcgaa ttgggacttg gaaccgttgt cgcggtggat gcgcgaactg tcactttact
    53881 tttcccatct actggtgaaa accgtctgta cgcacgcagt gattcccccg tgacccgcgt
    53941 gatgttcaac cctggtgata ccattaccag ccatgacggc tggcagatgc aagtcgaaga
    54001 agtaaaagaa gaaaatggct tgctgaccta tatcggtact cgcctggata ctgaagagtc
    54061 cggcgtagcc ctgcgtgaag ttttccttga tagcaaaatg gtgttcagca aaccgcagga
    54121 ccgcctgttt gccgggcaga ttgaccgtat ggaccgcttt gcgctgcgtt atcgcgcgcg
    54181 taagtattcc agcgaacagt tccgtatgcc gtacagcggc ctgcgcggtc agcgtaccag
    54241 cctgatcccg caccagctca acatcgctca tgatgttggc cgccgccacg cgccgcgcgt
    54301 cctgctggct gacgaagtgg gtttagggaa aaccattgaa gccgggatga tcctgcatca
    54361 gcaactgctc tctggcgctg ctgaacgtgt gctgattatc gtcccggaaa ccttacagca
    54421 tcagtggctg gtagaaatgc tgcgccgttt caacctgcgc tttgctctgt ttgatgatga
    54481 gcgttatgcc gaagctcagc acgatgctta caacccgttc gacaccgaac agctggtgat
    54541 ttgctcgctg gattttgccc gtcgtagcaa acagcgcctg gaacatctct gtgaagccga
    54601 atgggacctg ctggtggtcg atgaagcgca tcacctggtg tggagcgaag atgcgccaag
    54661 ccgtgaatat caggccattg aacaactggc agagcacgtg ccgggcgttc tgctgctgac
    54721 cgcgaccccg gaacagctgg ggatggaaag ccacttcgcc cgtctgcgtc tgctggaccc
    54781 gaaccgtttc cacgattttg cgcagttcgt tgaagagcag aaaaattatc gtccggttgc
    54841 ggacgccgtt gccatgctgc tggcaggtaa caaactgagc aatgacgaac tgaacatgct
    54901 cggcgagatg atcggcgagc aggatatcga gccgctgttg caggcagcaa acagcgacag
    54961 cgaagatgcc cagagcgccc gtcaggagct ggtttcgatg ctgatggatc gccacggcac
    55021 cagccgcgtg ctgttccgta acacgcgtaa cggtgtgaaa ggattcccga aacgcgagct
    55081 gcacaccatt aagctgccgc taccgacgca gtatcagacg gctattaaag tctccggcat
    55141 tatgggcgca cgtaaaagtg cggaagatcg tgctcgcgat atgctctacc cggagcgtat
    55201 ttatcaggaa tttgaaggtg ataacgccac ctggtggaac ttcgatccgc gcgttgagtg
    55261 gctgatgggc tacctgacca gccatcgctc tcagaaagtg ctggtgatct gcgccaaagc
    55321 tgccactgcg ctgcaactgg agcaggtact gcgcgaacgt gaaggtattc gcgctgcggt
    55381 gttccacgaa ggtatgtcga ttatcgaacg tgaccgcgct gccgcctggt ttgccgaaga
    55441 agacaccggc gcacaggtac tgctgtgctc agaaatcggt tctgaaggac gtaacttcca
    55501 gttcgccagc cacatggtga tgtttgacct gccattcaac ccggatctac tggagcagcg
    55561 tattggtcgt ctggatcgta tcggccaggc gcacgatatt cagatccatg tgccttatct
    55621 ggagaaaacc gctcagtcgg tgctggtgcg ctggtatcac gaaggtctgg atgcatttga
    55681 gcacacctgc ccgaccggac gcactattta cgatagcgta tacaacgatc tgattaacta
    55741 tctggcttca ccggatcaaa ccgaaggctt tgacgatctg atcaaaaact gccgcgagca
    55801 acatgaagcg ctgaaagcac agctggaaca gggtcgtgac cggctgctgg aaatccactc
    55861 caacggtggc gaaaaagccc aggcactggc agaaagcatt gaagagcagg atgacgatac
    55921 caacctgatc gccttcgcca tgaacctgct cgatattatc ggtatcaatc aggacgatcg
    55981 cggcgacaac atgatcgtgc tgacgccgtc cgatcatatg ctggtgccgg acttccctgg
    56041 cctgtcggaa gatggcatca ccatcacctt tgatcgtgaa gtggcgctgg cgcgtgaaga
    56101 tgcacagttt attacctggg agcatccgct gatccgcaac ggtctggatc tgatcctttc
    56161 tggcgatacc ggtagcagca cgatttcact gttaaaaaac aaagcgttgc cggtaggtac
    56221 gctgttggtg gaactgattt atgtggttga agcccaggct ccgaagcagt tgcagctcaa
    56281 ccgcttcctg ccaccgacgc cggtacgtat gctgctggat aaaaacggca acaacctggc
    56341 ggcgcaggta gagtttgaaa cctttaaccg ccagcttaac gcggttaacc gtcacaccgg
    56401 cagcaaactg gttaacgccg tgcagcagga tgttcacgct atccttcaac tgggtgaagc
    56461 gcagatcgag aaatctgccc gtgcattgat tgatgcagcg cgtaacgaag ccgacgaaaa
    56521 actgtctgcc gagctgtctc gtctggaagc tctgcgtgca gtgaacccga acattcgtga
    56581 cgacgaactg accgccattg agagcaaccg tcagcaggta atggaaagcc tggatcaggc
    56641 aggttggcgt ctggatgccc tgcgtttgat cgttgtaacg catcagtaac ggagccgaaa
    56701 atggggatgg aaaactacaa tccaccgcag gaaccctggt tggttatcct gtatcaggat
    56761 gaccacatta tggtggttaa caagccgagc ggtttgttgt ctgtgccggg gcgtctggaa
    56821 gagcacaaag acagcgtgat gacgcgcatt cagcgtgatt atccgcaggc agaatcggtg
    56881 catcgtctgg atatggctac cagcggcgtg attgtagtgg cgctgaccaa agccgcggag
    56941 cgggagttaa aacgccagtt ccgcgagcgc gagccgaaaa agcagtatgt ggcccgcgtc
    57001 tgggggcatc catcccctgc ggaaggtctg gtggatctgc cgctgatttg cgactggcca
    57061 aaccgcccga aacagaaagt ctgttacgaa acgggtaaac ctgcgcagac ggaatatgaa
    57121 gtggtggagt atgcgccgga taacacggca agagtggtgt taaaaccgat tactgggcgt
    57181 tcgcatcaat tgcgtgtgca tatgctggcg caggggcatc cgattctcgg cgatcgtttt
    57241 tatgcatcac cagaagcgag agcgatggca ccgcgtttgt tgctacatgc agagatgttg
    57301 acgattaccc atccggcgta tggcaatagt atgacgttta aagcgccagc ggatttttaa
    57361 tagtcacgct ggcgcagttc ggtaggcgga taaggcattc acgccgaatc cggcaattgt
    57421 gcgccgattg cctgatgcga tgcgggccgc gtcttatcag gcctacagtt taaaacttat
    57481 ttaaaccctt tctgctgctt tatcagctca tatgcctgct gaatttcctg cgctttctgc
    57541 ttcgccatct ccatcatctc aggcggcaaa cctttcgcca ccagcttatc gggatggtgt
    57601 tcactcatca gcttacggta ggctcgttta atggtggtcg catcatccgt cggcttcaca
    57661 cccagcacat tacaggcatc ttccagcgtc gggccacgct gcgcttgctg ccagttacca
    57721 ccgccagatt gctgctgata accgccgcca aactgtgcac cgccctgcat catgcgcaaa
    57781 aactggtcaa actgggcgcg ggagatccct aattcttctg caatgacata cagcaccgcc
    57841 cgttcattcg ggtgcagtga accatcagca aacgccgcct gaatctggat ctccagaaac
    57901 atacgaatta agtcaaaacg accaaagcag acactgcgaa actggcgcat cttttcgcgc
    57961 agcgggtaat tgtctgattt tcccacccgg aacgcgtttt gcgccgcagt acgggaagcg
    58021 ccatgaaggt tcattcggtc catcagctgg ctggcgatat gaatatcagc ctccgtgacg
    58081 cgacctttgg atttggttaa atgccccatc acttcaaaag tggtggcaaa aaacagcgcc
    58141 tgacgctcac gctggttggc gaaccacgcc attttacggc tacgggcttt atcaaacata
    58201 tggccaatta acaggcctaa cactacgccc caaaagccgc cgcccatcag taaggccacg
    58261 gccacgccaa tgatttttcc ccaatactgc atatattccc caaatcgaca cacggatatc
    58321 agggctatct cccacaatat aaaggtgctt ttaccgtttt ccggctgcag tcaattgtgg
    58381 gacatcgcct ataatttgca ttatcatacc tgtcattcaa tgccgtgcct aacaccactg
    58441 acgctattcg gacaggatta acactagcgt agagatgacg agtacgttag tctctgaccg
    58501 tttgtcacgc gcaacgttac cgatgatgga acaataaaat caacgtatga aaaaacgtat
    58561 ccccactctc ctggccacca tgattgccac cgccctttat agtcaacagg gactggcagc
    58621 cgacctcgcc tcacagtgca tgttgggcgt gccaagctat gaccgtcctc tggtacaggg
    58681 cgataccaat gacttacccg tgactatcaa tgctgaccac gcgaaagggg actacccgga
    58741 tgacgccgtg tttactggca gcgtggatat catgcagggt aacagccgtc tgcaggccga
    58801 cgaagtgcag ctccatcaaa aagaggcacc aggacaaccg gagccggtac gaaccgttga
    58861 tgcgctcggt aatgtccatt acgacgataa ccaggtgatc ctcaaagggc cgaaaggctg
    58921 ggcgaatctg aacaccaaag ataccaacgt ctgggaaggt gattaccaga tggtgggtcg
    58981 ccagggccgc ggtaaagcgg acctgatgaa acaacgtggt gaaaaccgct ataccattct
    59041 ggataacggt agctttacct cctgtctgcc gggttctgac acctggagcg tggtaggtag
    59101 cgaaattatt catgaccgcg aagaacaagt tgcggagatc tggaacgccc gctttaaggt
    59161 gggtccagta ccgatctttt atagccccta tttgcagttg ccggtgggtg acaaacgtcg
    59221 ctctggtttc ttgatcccga acgccaagta caccaccacc aactactttg agttctacct
    59281 gccatattac tggaacatcg cgccaaatat ggatgccacc atcacgccgc attatatgca
    59341 tcgtcgtggc aacatcatgt gggagaacga attccgctac ctctcccagg cgggcgctgg
    59401 cttgatggaa ctggactatc tgccttcaga taaagtctat gaagatgaac acccgaacga
    59461 tgacagttca cgtcgttggt tgttctactg gcaacactcc ggggtcatgg atcaagtgtg
    59521 gcgtttcaac gtcgactaca ccaaggtcag cgatcctagc tacttcaatg atttcgataa
    59581 caagtacggt tccagtactg acggctacgc aacgcaaaaa ttcagcgttg gctatgcggt
    59641 gcaaaacttc aatgccaccg tttcaaccaa gcagttccag gtctttagcg agcagaacac
    59701 cagtagctac tcggcagagc cgcagttaga cgttaactac taccagaatg atgttggtcc
    59761 gtttgatacg cgtatttacg gccaggcagt gcactttgtt aacaccagag acgacatgcc
    59821 tgaagcaacc cgtgttcacc tggaaccgac catcaatttg ccgctctcta ataactgggg
    59881 cagcatcaat accgaagcga agttgctggc aacccattat cagcaaacca atcttgactg
    59941 gtataactcc agaaacacga ccaagctgga cgaatccgtt aaccgcgtta tgccgcaatt
    60001 caaagttgac ggcaaaatgg tctttgaacg cgatatggaa atgctggctc cgggttatac
    60061 ccaaacgctg gaaccgcgcg cgcagtattt gtacgtgccg tatcgcgatc agagcgacat
    60121 ctataactac gactcgtctc tactgcaatc tgactactct ggcctgttcc gggaccggac
    60181 ttacggcggt cttgaccgta ttgcctccgc taaccaggtg acgaccggtg tcacatctcg
    60241 catatatgat gatgctgccg ttgaacattt taatatttcc gttggtcaaa tctactattt
    60301 cacggagtct cgcactggcg atgacaacat aacatgggag aatgacgaca aaacgggctc
    60361 actggtgtgg gcaggcgata cttactggcg tatctccgag cgttggggat tgcgtggcgg
    60421 gattcagtac gatacacgtc tggataacgt agcgaccagt aactccagca ttgaataccg
    60481 tcgggatgaa gaccgtctgg tacagctgaa ttaccgttac gccagcccgg aatatattca
    60541 ggctacgctg cctaagtact attccactgc tgagcaatat aagaatggta tttcgcaggt
    60601 aggtgctgtc gccagctggc caattgccga tcgttggtcc attgttgggg cctactacta
    60661 cgacaccaat gcgaacaagc aagccgactc tatgttaggt gtgcaataca gctcctgctg
    60721 ctatgcaatt cgcgtcggtt acgagcggaa gctgaacggt tgggataacg ataaacaaca
    60781 tgcggtatat gacaacgcaa tcggctttaa catcgaactt cgcggcctga gctccaacta
    60841 cggtctgggt acgcaagaga tgctgcgttc gaacattctg ccgtatcaaa acactttgtg
    60901 atttgttgat ttaccacgta atccgcagtg cggttaattg aaatggaaaa agtatgaaga
    60961 actggaaaac gctgcttctc ggtatcgcca tgatcgcgaa taccagtttc gctgcccccc
    61021 aggtagtcga taaagtcgca gccgtcgtca ataacggcgt cgtgctggaa agcgacgttg
    61081 atggattaat gcagtcggta aaactgaacg ctgcgcaggc aaggcagcag cttcctgatg
    61141 acgcgacgct gcgccaccaa atcatggaac gtttgatcat ggatcaaatc atcctgcaga
    61201 tggggcagaa aatgggagtg aaaatctccg atgagcagct ggatcaggcg attgctaaca
    61261 tcgcgaaaca gaacaacatg acgctggatc agatgcgcag ccgtctggct tacgatggtc
    61321 tgaactacaa cacctatcgt aaccagatcc gcaaagagat gattatctct gaagtgcgta
    61381 acaacgaggt gcgtcgtcgc atcaccatcc tgccgcagga agtcgaatcc ctggcgcagc
    61441 aggtgggtaa ccaaaacgac gccagcactg agctgaacct gagccacatc ctgatcccgc
    61501 tgccggaaaa cccgacttct gatcaggtga acgaagcgga aagccaggcg cgcgccattg
    61561 tcgatcaggc gcgtaacggc gctgatttcg gtaagctggc gattgctcat tctgccgacc
    61621 agcaggcgct gaacggcggc cagatgggct ggggccgtat tcaggagttg ccgggtatct
    61681 tcgcccaggc attaagcacc gcgaagaaag gcgacattgt tggcccgatt cgttccggcg
    61741 ttggcttcca tattctgaaa gttaacgacc tgcgcggcga aagcaaaaat atctcggtga
    61801 ccgaagttca tgctcgccat attctgctga aaccgtcgcc gatcatgact gacgaacagg
    61861 cccgtgtgaa actggaacag attgctgccg atatcaagag tggtaaaacg acttttgctg
    61921 ccgcagcgaa agagttctct caggatccag gctctgctaa ccagggcggt gatctcggct
    61981 gggctacacc agatattttc gatccggcct tccgtgacgc cctgacccgc ctgaacaaag
    62041 gtcaaatgag tgcaccggtt cactcttcat tcggctggca tttaatcgaa ctgctggata
    62101 cccgtaatgt cgataaaacc gacgctgcgc agaaggatcg tgcataccgc atgctgatga
    62161 accgtaagtt ctcggaagaa gcagcaagct ggatgcagga acaacgcgcc agcgcctacg
    62221 ttaaaatcct gagcaactaa tggttaaaac ccaacgtgtt gtgatcactc ccggcgagcc
    62281 cgccgggatt ggcccggact tagttgtcca gcttgcacag cgtgagtggc cggtcgaact
    62341 ggttgtttgt gccgatgcca ctctccttac cgaccgggca gcgatgctcg gtttgccgct
    62401 caccctccgc ccttattccc ccaactcccc tgcacaaccg caaactgcgg gcacattaac
    62461 gctacttcct gtcgcgctac gcgaatctgt cactgcgggg cagttagcga ttgaaaatgg
    62521 gcattacgtg gtggagacgc tggcgcgagc gtgtgatggc tgtctgaacg gtgaatttgc
    62581 tgcgctgatc acaggccccg tgcataaagg cgtcattaac gacgcaggta ttccgtttac
    62641 tggtcatacc gagtttttcg aagagcgctc gcaggcgaaa aaagtggtga tgatgctggc
    62701 gaccgaagaa cttcgcgtgg cgctggcaac gacgcattta ccgctgcgcg atatcgcaga
    62761 tgctatcacc cctgcacttt tgcacgaagt gattgctatt ttgcatcacg atttgcgtac
    62821 aaaatttggt attgccgaac cgcgcattct ggtctgcggg ctgaatccgc acgcgggcga
    62881 aggcggtcat atgggtacgg aagagataga caccattatt ccggtgctcg acgagctgcg
    62941 ggcgcagggg atgaaactca acgggccgct gcctgccgat accctgtttc agccgaaata
    63001 tctcgataac gccgacgccg tgctggcgat gtaccacgat cagggtcttc ccgtgctaaa
    63061 ataccagggc ttcgggcgcg gtgtgaacat tacgctgggc ctgcccttta ttcgcacatc
    63121 agtggaccac ggcaccgcgc ttgaactggc gggacgtggc gaagccgatg tcggcagttt
    63181 tattacggcg cttaatctcg ccatcaaaat gattgttaac acccaatgaa taatcgagtc
    63241 caccagggcc acttagcccg taaacgcttc gggcaaaact ttctcaacga tcagttcgtg
    63301 atcgacagca ttgtttctgc cattaacccg caaaagggcc aggcaatggt cgaaatcggc
    63361 cccggtctgg cggcattgac cgagccggtc ggcgaacgtc tggaccagct gacggtcatc
    63421 gaacttgacc gcgatctggc ggcacgtctg caaacgcatc cattcttagg cccgaaactg
    63481 acgatttatc agcaggatgc gatgaccttt aactttggcg aactggccga gaaaatgggt
    63541 cagccgctgc gtgttttcgg caacctgcct tataacatct ccacgccgtt gatgttccat
    63601 ctgtttagct atactgatgc cattgccgac atgcacttta tgttgcaaaa agaggtggtg
    63661 aatcgtctgg ttgcaggacc gaacagcaaa gcgtatggtc gattaagcgt catggcgcaa
    63721 tactattgca acgtgatccc ggtactggaa gttccgccgt cagcctttac accaccaccc
    63781 aaagtggatt ccgccgtcgt gcgcctggtt cctcatgcaa cgatgcctca cccggttaaa
    63841 gatgttcgtg tgttgagccg catcaccacc gaagccttta accagcgtcg taaaaccatt
    63901 cgtaacagcc tcggcaacct gtttagcgtc gaggtgttaa cgggaatggg gatcgacccg
    63961 gcgatgcgag cggaaaatat ctctgtcgcg caatattgcc agatggcgaa ctatctggcg
    64021 gagaacgcgc ctttgcagga gagttaacga tgatcaattc gccccgagtg tgtattcagg
    64081 ttcaaagcgt ctacattgag gcacaatctt cacctgataa tgaacgttac gtttttgctt
    64141 ataccgtaac catacgcaat ctggggcgag cgccagtgca gttgttgggg cgttactggc
    64201 tgatcaccaa tggcaatggc cgtgaaaccg aagtccaggg cgaaggagtg gttggcgtcc
    64261 agccacttat cgcgcctggc gaagagtacc agtacaccag cggtgcaatc attgaaaccc
    64321 cgctgggcac catgcagggt cactacgaaa tgatcgatga aaatggcgtc cctttcagca
    64381 tcgacattcc cgtattccga ctcgccgttc ccacactcat tcattaaaag aatatggcga
    64441 cataccttat tggcgacgtt catggttgtt acgatgaact gatcgcattg ctgcataaag
    64501 tagaatttac ccctgggaaa gataccctct ggctgacggg cgatctggtc gcgcgcggcc
    64561 cgggttcgct ggatgttctg cgctatgtga aatccttagg cgacagcgta cgtctggtgc
    64621 tgggcaatca cgatctgcat ctgctggcgg tatttgccgg gatcagccgc aataaaccga
    64681 aagatcgcct gacaccgctg ctggaagcgc cggatgccga cgagctgctt aactggctgc
    64741 gtcgccagcc tttgctgcaa atcgacgaag agaaaaagtt ggtgatggcc cacgcaggga
    64801 tcacgccgca gtgggatcta cagaccgcca aagagtgcgc gcgcgatgta gaagcggtgc
    64861 tatcgagtga ctcctatccc ttctttctcg acgctatgta cggcgatatg ccaaataact
    64921 ggtcaccgga attgcggggg ctgggaagac tgcgttttat caccaacgcc tttacccgta
    64981 tgcgtttttg cttcccgaac ggtcaactgg atatgtacag caaagaatcg ccggaagagg
    65041 cccctgcccc actgaaaccg tggtttgcga ttcctggccc tgtcgctgaa gaatacagca
    65101 tcgcctttgg tcactgggca tcgctggagg gcaaaggtac gccggaaggt atatacgcgc
    65161 tggataccgg ctgctgctgg ggtggtacat taacctgcct gcgctgggaa gataaacagt
    65221 attttgtcca gccgtcgaac cggcataagg atttgggcga agcggcggcg tcttaaacac
    65281 agcctgatat aggaaggccg gataagacgc gaccggcgtc gcatccggca ctagccgtaa
    65341 attctataca aaattaccgc cgctccagaa tctcaaagca atagctgtga gagttctgcg
    65401 catcagcatc gtggaattcg ctgaataccg attcccagtc atccggctcg taatccggga
    65461 aatgggtgtc gccttccact tctgcgtcga tatgcgtcag atacagtttc tgcgcttttg
    65521 gcaggaactg ctcataaacg cgaccgccgc caatcaccat gatttctggt acgtcaccac
    65581 acgccgcgat ggcttcatcc accgacttca cccacgttac gcgatcgtcc gtacccggtt
    65641 gactgctgag gataatattt ttgcgtcctg gcaacggacg accgattgat tcccaggtat
    65701 ggcggcccat aatcacgggt ttatttaagg tgttgcgttt aaaccaggcg agatcggcag
    65761 gcaggttcca cggcatggcg ttttccatgc cgataacgcg atctaccgct aacgccgcaa
    65821 tcagactgat cattgagatt tcccgataaa aaaaattgtc gccactatac gtaaagcgta
    65881 aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat tttatattct gctggcgagt
    65941 ccacgctctc tccctggact cgccgcatta caatgaaaca aaaacaaaca gttagctgta
    66001 aagtgtgatt tacgtcactc tttattagga tgagggtttc gtttccggtt catccgacat
    66061 gttgccggta tgtttacctt cttcggttcc ctgccagcca tgtcgttgaa ttaatgacag
    66121 atgttcgcgg tcctcggtaa tgatctcact taacatcgcg ctggtgcgtt tatagacctc
    66181 cgcgcgggct ttggtgtcgt tctcaaccat tgccatctct tccaccatct gaatattaaa
    66241 gcggcggaac acatcggcac gttctcgcgc ttcatacggc cccagaccta aactttccag
    66301 tgccagacgc ccggttttca gcgcaccttc gaaggtttca cgctccggct tttcaacgcc
    66361 tgcctgacgc aaacgaatgt agtggtcgac atcgcgggcg cgggcaataa tctgcaaatg
    66421 cgggaagtgt tctttcacca tctctgtcag ttgcaggttg gtttgcggat cgtcgatggc
    66481 gttaatcagc acttccgctt tcgccgctcc cgcagattcc agtaaatcca tccgcgtggc
    66541 atcgccataa aacactttca taccaaattt acgcaaggtt tcgatatggt ccggatcgtg
    66601 atcgagcacc accattttca ccccgctgga gagcagtaaa cgtccggtga tctgcccaaa
    66661 acgaccgaat ccggcaatga ttacccgcgg ctgttcttcg tcgatctcat cggcttcacg
    66721 cgcctcttca gtagaagatt gctcaaggcg attgaggatc accagcagaa tcggcgttgc
    66781 cgccatcgac agcgccaccg ccagggtcag cgatttcgcc cactccggct ccagcacatt
    66841 cgccatctgc gccgcgccaa ataccacaaa ggcaaactca ctgccctgcc ctaacaacac
    66901 cgcaaaccaa cgacgctgtt tatttggcac ttgcaacggt cgggcaatca gccacagcat
    66961 ggcgattttg atgatgagga aaccgagcag caaaatgaca atgcgcaatg ggttttcaag
    67021 cagcgtgcca aagtctatcg acatgccaac accgatgaaa aacagcccca acagcaaacc
    67081 tttaaagggt tcgatatcgc tctccagcgc atgacggtat tccgagcttg ccagcagtac
    67141 gcccgccaga aacgcgccca tcgccatcga caagccgacc tcttccagca gcaaaccaaa
    67201 gccaaacacg aggaataacg ccacggcact aaacacttcc cgcaagccag agcgggctac
    67261 aaaacgcagc gccggacgcg tgacatagcg ccccagcaat accaccagca ccagcgcacc
    67321 cgccactttt aacgccgaga gagcaaatgc gcccatcgtc gtcgaggcac tgctcgttgc
    67381 cagtagcgga atcatcgcca ccagcgggat cgccgcgata tcctggaaca gcagcaccgc
    67441 aaaggcactg cgacccattt gcgtcaccat cagattgcgt tcattcatcg cctgcatggc
    67501 aatcgccgta gaggagagcg ccagcgtcat gccgatcaat tccgcgacct gccagcgcag
    67561 cccaagtaac atgcagaaca gccccagcag gccgccgcaa atcaccatct gcaatgcgcc
    67621 accgccgaac actgccgcac gcagcttcca cagcctttgt ggatcgagtt cgaggccgat
    67681 aataaacagc atcagcacca ccccaatctc ggcaaagtgc agaatagatt cggcatcggt
    67741 caccagtcgc agcccccacg ggccaataat gcagccggcg atcaggtagc caagtaccga
    67801 tcccagacca agacgtaccg caatgggtac aatcagcgct gccgaaccga gataaatcag
    67861 cgcctgaatc agcgtatggc tatccatgat gggcctcctg ccattccagc agacgttgct
    67921 tatagtgacg cgcctgccct tcgagggttt cgtcgtcaca aataaaggtg cagtgcatgg
    67981 caaacggtgg cagccagttc agcccgcagt agattgccgt cgcctgtagc ggctgcgaca
    68041 gcacatcaaa gcccggatgc gcaccaattt caaaatggct ttccccgccg ccggtcgtca
    68101 ccgcccacag caaatgtttg ccatgcagcg ccttgccgcc gtgaccgtaa gcccagccgt
    68161 gcgacaaaac tttatcgatc caaagtttaa gaagcggagg aatgctgtac cactgcatcg
    68221 gatgctgcca gacgatcaga tcggcgcgag acagcgcctc ctgctcggcg gcaatatcga
    68281 tattgaagtc aggatagagt tgataaagag agcgaatttc gacgccttcc agcgtcctgc
    68341 ctgttcaagc atccgtttat tcgcatggga atgatgcgga tacggatgcg cataaattat
    68401 aagaatcata ggtagcccgc gtgattacgc ctttgttttc ccacaaagtg tagtcatata
    68461 ttcacgcggc ttatagtcaa taatattgat tcagaaaaga gagaaatctg aacaatttac
    68521 cccattctct gcccgcggat cgtcatattg ctggtttgcg ccagtgacat ccctcgatgt
    68581 ctccggggca aacgctacgg aaatcaacaa gccaaacagc gagatacccg cccccattag
    68641 catcgtgtta ctgataccgt aattattgat aaagatcggc agtgcccagg tcgaaacaat
    68701 ggtgccaata cggcttaagg acataatcac gcccacggca gaggcgcgga tatctgtcgg
    68761 gaagagttca ttaggataga gccactgcaa attacccggc ccgccagaga aaaaggcata
    68821 caccgcaaag gccatcacta ccagccagat ccccatatcc gggatcagcc ccaaaaccgc
    68881 cagcgccagc gtcatcatgg caaagctgcc aatcaacaat ggacgccgtc cggcagtgtt
    68941 taaccacagc atcggcggaa tacagtcgag cataaagaac aggctaatca ccacgttccc
    69001 cagcgccgcg tttttgccaa cacccaatcc caacaaacca acgatttgcg ggccaaaggt
    69061 gtaaatggcg aacattggga tcacctggca ggtccagatg gcggcaacaa acagaacaaa
    69121 aggaaaatgg cggcgattaa acagatcgcg aaaacgggtt tgctgcggct gctcttcatc
    69181 gaaagccacc ggttcgccaa acagttttat catcatctct tcgcactctt ttactcgccc
    69241 tttgcgtaat aaccagcggg gagattcagg cagttcgaat cgaccaatca aaatcaacaa
    69301 acaggggatc gccgcgctac ccagcatcca gcgccagccg ccttccacat cataaagcca
    69361 gtagccgacc agatcggcgc aggtcgcgcc gacataccac atggcggcga taaagctgat
    69421 ggaaaatgcc cgctgacggg tactggagaa ctcggtgatc attgaggtgg cgatgggata
    69481 atctgcaccg atgacaatgc cgataagtac ccgcatcacc aacagttcga cgggggatga
    69541 aacaaacatc gtcgccaccg atatcacgcc gatggcgatg atatcaatga ggaacatttt
    69601 gcgccgtccg actttatcgg aaatatagcc aaacagcgat gtgccaacga acagcccggc
    69661 gagcgttccc gcgcccaaca agccaatcca gtcagcgtcc agttttagcg ccggagtcag
    69721 ttgctccagc gccacgccaa tcattaccag tacataacca tccagaa
//
LOCUS       DAJTNA010000002        60051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTNA010000002 DAJTNA010000000
VERSION     DAJTNA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60051
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.002"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(2..94)
                     /locus_tag="OO161_000064"
     tRNA            complement(2..94)
                     /locus_tag="OO161_000064"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO161_000065"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO161_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5461279.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO161_000066"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO161_000066"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5461280.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKDVEIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFDAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO161_000067"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO161_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5461281.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO161_000068"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO161_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5461282.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARIMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO161_000069"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO161_000069"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5461283.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO161_000070"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO161_000070"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5461284.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVGFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8018
                     /gene="srlA"
                     /locus_tag="OO161_000071"
                     /gene_synonym="gutA"
     CDS             7455..8018
                     /gene="srlA"
                     /locus_tag="OO161_000071"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR5461285.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8015..8974
                     /gene="srlE"
                     /locus_tag="OO161_000072"
                     /gene_synonym="gutE"
     CDS             8015..8974
                     /gene="srlE"
                     /locus_tag="OO161_000072"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5461286.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8985..9356
                     /gene="srlB"
                     /locus_tag="OO161_000073"
                     /gene_synonym="gutB"
     CDS             8985..9356
                     /gene="srlB"
                     /locus_tag="OO161_000073"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5461287.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAVFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9360..10139
                     /gene="srlD"
                     /locus_tag="OO161_000074"
     CDS             9360..10139
                     /gene="srlD"
                     /locus_tag="OO161_000074"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5461288.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10244..10603
                     /gene="gutM"
                     /locus_tag="OO161_000075"
     CDS             10244..10603
                     /gene="gutM"
                     /locus_tag="OO161_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5461289.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10670..11443
                     /gene="srlR"
                     /locus_tag="OO161_000076"
                     /gene_synonym="gutR"
     CDS             10670..11443
                     /gene="srlR"
                     /locus_tag="OO161_000076"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5461290.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11436..12401
                     /gene="gutQ"
                     /locus_tag="OO161_000077"
     CDS             11436..12401
                     /gene="gutQ"
                     /locus_tag="OO161_000077"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5461291.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGSALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12398..13912)
                     /gene="norR"
                     /locus_tag="OO161_000078"
     CDS             complement(12398..13912)
                     /gene="norR"
                     /locus_tag="OO161_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5461292.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPSDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14099..15538
                     /gene="norV"
                     /locus_tag="OO161_000079"
     CDS             14099..15538
                     /gene="norV"
                     /locus_tag="OO161_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5461293.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECFLGKDVFEELASEAK"
     gene            15535..16668
                     /gene="norW"
                     /locus_tag="OO161_000080"
     CDS             15535..16668
                     /gene="norW"
                     /locus_tag="OO161_000080"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5461294.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDYAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPTMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="OO161_000081"
     CDS             complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="OO161_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5461295.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGLRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEASLQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="OO161_000082"
     CDS             complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="OO161_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5461296.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19877..20887)
                     /gene="ascG"
                     /locus_tag="OO161_000083"
     CDS             complement(19877..20887)
                     /gene="ascG"
                     /locus_tag="OO161_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5461297.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            21147..22604
                     /gene="ascF"
                     /locus_tag="OO161_000084"
                     /pseudo
     CDS             21147..22604
                     /gene="ascF"
                     /locus_tag="OO161_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
     gene            22613..24037
                     /gene="ascB"
                     /locus_tag="OO161_000085"
     CDS             22613..24037
                     /gene="ascB"
                     /locus_tag="OO161_000085"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR5461298.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24191..24661)
                     /gene="hycI"
                     /locus_tag="OO161_000086"
     CDS             complement(24191..24661)
                     /gene="hycI"
                     /locus_tag="OO161_000086"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR5461299.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(24654..25064)
                     /gene="hycH"
                     /locus_tag="OO161_000087"
     CDS             complement(24654..25064)
                     /gene="hycH"
                     /locus_tag="OO161_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR5461300.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25061..25828)
                     /gene="hycG"
                     /locus_tag="OO161_000088"
     CDS             complement(25061..25828)
                     /gene="hycG"
                     /locus_tag="OO161_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR5461301.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQQLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            complement(25828..26370)
                     /gene="hycF"
                     /locus_tag="OO161_000089"
     CDS             complement(25828..26370)
                     /gene="hycF"
                     /locus_tag="OO161_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR5461302.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(26380..28089)
                     /gene="hycE"
                     /locus_tag="OO161_000090"
     CDS             complement(26380..28089)
                     /gene="hycE"
                     /locus_tag="OO161_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5461303.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTGAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGSAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDYGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTHQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPVEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28107..29030)
                     /gene="hycD"
                     /locus_tag="OO161_000091"
     CDS             complement(28107..29030)
                     /gene="hycD"
                     /locus_tag="OO161_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5461304.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29033..30899)
                     /gene="hycC"
                     /locus_tag="OO161_000092"
                     /pseudo
     CDS             complement(29033..30899)
                     /gene="hycC"
                     /locus_tag="OO161_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            complement(30896..31507)
                     /gene="hycB"
                     /locus_tag="OO161_000093"
     CDS             complement(30896..31507)
                     /gene="hycB"
                     /locus_tag="OO161_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5461305.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNVITRVDGAVQLNESLCVSCQLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(31632..32093)
                     /gene="hycA"
                     /locus_tag="OO161_000094"
     CDS             complement(31632..32093)
                     /gene="hycA"
                     /locus_tag="OO161_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5461306.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32305..32655
                     /gene="hypA"
                     /locus_tag="OO161_000095"
     CDS             32305..32655
                     /gene="hypA"
                     /locus_tag="OO161_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5461307.1"
                     /translation="MHAITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            32659..33531
                     /gene="hypB"
                     /locus_tag="OO161_000096"
     CDS             32659..33531
                     /gene="hypB"
                     /locus_tag="OO161_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5461308.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            33522..33794
                     /gene="hypC"
                     /locus_tag="OO161_000097"
     CDS             33522..33794
                     /gene="hypC"
                     /locus_tag="OO161_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5461309.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            33794..34915
                     /gene="hypD"
                     /locus_tag="OO161_000098"
     CDS             33794..34915
                     /gene="hypD"
                     /locus_tag="OO161_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5461310.1"
                     /translation="MRFVDEYRTPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIEAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            34912..35922
                     /gene="hypE"
                     /locus_tag="OO161_000099"
     CDS             34912..35922
                     /gene="hypE"
                     /locus_tag="OO161_000099"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5461311.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            35996..38064
                     /gene="flhA"
                     /locus_tag="OO161_000100"
                     /pseudo
     CDS             35996..38064
                     /gene="flhA"
                     /locus_tag="OO161_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(38101..38454)
                     /locus_tag="OO161_000101"
     CDS             complement(38101..38454)
                     /locus_tag="OO161_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5461312.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKARE"
     gene            complement(38531..38665)
                     /locus_tag="OO161_000102"
     CDS             complement(38531..38665)
                     /locus_tag="OO161_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461313.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            38740..41301
                     /gene="mutS"
                     /locus_tag="OO161_000103"
     CDS             38740..41301
                     /gene="mutS"
                     /locus_tag="OO161_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5461314.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            41407..42063
                     /gene="pphB"
                     /locus_tag="OO161_000104"
                     /pseudo
     CDS             41407..42063
                     /gene="pphB"
                     /locus_tag="OO161_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(42114..42881)
                     /locus_tag="OO161_000105"
     CDS             complement(42114..42881)
                     /locus_tag="OO161_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5461315.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            43077..43985
                     /locus_tag="OO161_000106"
     CDS             43077..43985
                     /locus_tag="OO161_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5461316.1"
                     /translation="MKTGSEFHVGIVSLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVVQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            43982..45244
                     /locus_tag="OO161_000107"
     CDS             43982..45244
                     /locus_tag="OO161_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5461317.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            45241..45879
                     /locus_tag="OO161_000108"
     CDS             45241..45879
                     /locus_tag="OO161_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5461318.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEATNNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            45884..46660
                     /locus_tag="OO161_000109"
     CDS             45884..46660
                     /locus_tag="OO161_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5461319.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            46749..48113
                     /locus_tag="OO161_000110"
     CDS             46749..48113
                     /locus_tag="OO161_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR5461320.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAVFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAAI"
     gene            complement(48207..49199)
                     /gene="rpoS"
                     /locus_tag="OO161_000111"
                     /pseudo
     CDS             complement(48207..49199)
                     /gene="rpoS"
                     /locus_tag="OO161_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
     gene            complement(49262..50353)
                     /gene="nlpD"
                     /locus_tag="OO161_000112"
     CDS             complement(49262..50353)
                     /gene="nlpD"
                     /locus_tag="OO161_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5461321.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(50493..51119)
                     /gene="pcm"
                     /locus_tag="OO161_000113"
     CDS             complement(50493..51119)
                     /gene="pcm"
                     /locus_tag="OO161_000113"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5461322.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(51113..51874)
                     /gene="surE"
                     /locus_tag="OO161_000114"
     CDS             complement(51113..51874)
                     /gene="surE"
                     /locus_tag="OO161_000114"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5461323.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(51855..52904)
                     /gene="truD"
                     /locus_tag="OO161_000115"
     CDS             complement(51855..52904)
                     /gene="truD"
                     /locus_tag="OO161_000115"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5461324.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(52901..53380)
                     /gene="ispF"
                     /locus_tag="OO161_000116"
     CDS             complement(52901..53380)
                     /gene="ispF"
                     /locus_tag="OO161_000116"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5461325.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(53380..54090)
                     /gene="ispD"
                     /locus_tag="OO161_000117"
     CDS             complement(53380..54090)
                     /gene="ispD"
                     /locus_tag="OO161_000117"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5461326.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(54109..54420)
                     /gene="ftsB"
                     /locus_tag="OO161_000118"
     CDS             complement(54109..54420)
                     /gene="ftsB"
                     /locus_tag="OO161_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5461327.1"
                     /translation="MGKLTLLLLAILIWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(54614..54937)
                     /locus_tag="OO161_000119"
     CDS             complement(54614..54937)
                     /locus_tag="OO161_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5461328.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(54987..55592)
                     /gene="cysC"
                     /locus_tag="OO161_000120"
     CDS             complement(54987..55592)
                     /gene="cysC"
                     /locus_tag="OO161_000120"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5461329.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGVLEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(55592..57019)
                     /gene="cysN"
                     /locus_tag="OO161_000121"
     CDS             complement(55592..57019)
                     /gene="cysN"
                     /locus_tag="OO161_000121"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5461330.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(57021..57929)
                     /gene="cysD"
                     /locus_tag="OO161_000122"
     CDS             complement(57021..57929)
                     /gene="cysD"
                     /locus_tag="OO161_000122"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5461331.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWAP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            58181..59218
                     /gene="iap"
                     /locus_tag="OO161_000123"
     CDS             58181..59218
                     /gene="iap"
                     /locus_tag="OO161_000123"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5461332.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTTEVVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(59425..59718)
                     /gene="cas2"
                     /locus_tag="OO161_000124"
     CDS             complement(59425..59718)
                     /gene="cas2"
                     /locus_tag="OO161_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR5461333.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(59718..>60051)
                     /locus_tag="OO161_000125"
     CDS             complement(59718..>60051)
                     /locus_tag="OO161_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="subtype I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCR5461334.1"
                     /translation="AAGYAPAIGFIHSGKPLSFVYDIADIIKFDSVVPKAFEIAARQP
                     AEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGEIEPPQPAPDMLPPAIPEPETLG
                     DSGHRGRG"
BASE COUNT        14201 a        16424 c        15502 g        13924 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt cacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggtgtttgat
     1981 ctagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcgtcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatttcg acgtcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactgattca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc tagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggacgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtgtgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatt atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 ccccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggtg tccggctggg
     5041 agcacagcag gttgtcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaagcaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcgcctatca ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttca
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgcaaa tagttactgc
     6121 acgcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttataac gggtaatggt gtagaagttc ggcagaccgt accagtactg gtaaccggta
     6241 ccgacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgccg tggcaagctg cgaaatgctg tacctggttt tgaagccatt tggcaagcct
     6361 ggagcctgac cgtttgccat taccgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaacccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac gccccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tccttttact taaactttca aattaaaata tttatctttc atttttagat caaaatatca
     7381 cttttaaatc tttcaatctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgctggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct gctgctaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctctttcccc
     7801 atatcaaccc tggcgaactg tttgtttatc ttggcattgc cagcggtctg acaacgctga
     7861 acctgccact tggcccactg gcggtgagct atctgctggt tggtctggtc accaacttct
     7921 ttcgcggctg ggtgaccgat ctgaccaccg ccatttttga gaaaaagatg ggcattcaac
     7981 ttgaacaaaa agttcacctg gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg
     8041 aacgggtggc tggggcggcc cgcttgagct ggaagccacg ccgggcaaaa aaatcgtcta
     8101 tatcaccgcc ggtacccggc ctgcgattgt tgacaaactg gcacaactta ctggctggca
     8161 ggctattgac ggatttaaag aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat
     8221 cgactgtggc ggcacattac gctgcggcat ctatccgaaa cgacgtattc ccaccattaa
     8281 tatccactcg acgggcaagt ccggtccgct ggcgcagtac attgtggaag atatttatgt
     8341 ctctggcgta aaagaagaaa acatcactgt agtgggtgat gcgacaccac aaccctcttc
     8401 cgtgggccgt gactatgaca ccagtaagaa aatcaccgaa caaagcgatg gtttactggc
     8461 gaaggtggga atgggcatgg ggtccgccgt tgcggtgctg tttcaatctg gtcgtgacac
     8521 catcgacact gtattaaaaa ccattctgcc gtttatggca ttcgtctcgg cgctcattgg
     8581 catcattatg gcttctggcc ttggtgactg gattgcccac ggtcttgctc cgctggcgag
     8641 ccatccactg ggtctggtca tgctggcgct catctgctcc ttcccactgc tttcaccttt
     8701 cctcggccca ggcgcagtta tcgcacaggt tatcggcgta ttgattggcg tgcagattgg
     8761 tctcggcaat attccgccgc atctggcttt accggcactg tttgccatca acgcgcaggc
     8821 ggcctgcgac ttcatcccgg tcggtttgtc gctggcggaa gcccgtcagg acacggttcg
     8881 cgtcggtgtc ccttctgtac tggtgagccg ctttttaacc ggcgcaccga ctgtactgat
     8941 cgcctggttt gtctccggtt ttatctatca atagaggctg aaacatgacc gttatttata
     9001 agaccaccat cacccgtatc ggcgcgagtg ccactgacgc cctcagcgac cagatgctca
     9061 tcacctttcg tgaaggcgcg ccagcggacc tcgaagagta ttgcttcatt cattgccacg
     9121 gcgagttgaa aggtgcactc catcccggtt tgcaatttac gctcgggcag catcgctatc
     9181 cggtgaccgc tgttggcagc gtggcggaag acaaccttcg cgaactgggt catgtcaccc
     9241 tgcgcttcga tggtttaaac gaagcggtat ttccgggcac tgtccatgtg gcaggccctg
     9301 tacccgacga tatcgcgccg ggatcggttt tgaagtttga atctgttaag gagtaaaaaa
     9361 tgaatcaggt tgccgttgtc atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg
     9421 gtctggttgc cgaggggtat cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa
     9481 atgtggcaca agaaattaac gccgaatatg gtgaaagtat ggcgtacggt tttggtgctg
     9541 acgccactag cgagcaaagc gttctggcgc tctctcgtgg ggtagatgaa atctttggtc
     9601 gcgtggattt gctggtctat agcgccggaa tagcaaaagc agcctttatc agcgacttcc
     9661 agctcggcga ttttgaccgt tcgctacaag tgaatctggt gggttatttc ctgtgtgccc
     9721 gtgaattttc gcgtttgatg atccgcgacg ggattcaggg tcgcattatt cagatcaact
     9781 cgaaatccgg caaagtgggc agcaaacaca actctggcta cagcgcagcg aaatttggtg
     9841 gcgtcgggct gactcaatca ctggcgctgg atctggcgga gtacggcatt acggtgcatt
     9901 cactgatgct cggtaacctg ctgaaatcgc cgatgttcca gtcactgttg ccacaatacg
     9961 cgaccaagct gggtatcaaa ccggatcaag tcgagcagta ttacatcgac aaagtaccgc
    10021 tcaaacgcgg ctgcgattat caagatgtgc tgaatatgct gctgttctac gccagtccta
    10081 aggcgtcgta ctgcaccgga cagtcgatca atgtcaccgg cggtcaggtg atgttctgat
    10141 caacagcgga gatccattaa ggatctccgt gagactatag aatgcctgat gcgctacgct
    10201 catcaggcat acaggacttc cgccactaca ttaaggaaaa gttatggtat ccgcactcat
    10261 caccgtcgcc gttatcgcct ggtgtgcgca actggcctta ggcggctggc aaatttctcg
    10321 ttttaaccgt gccttcgaca cactatgcca gcaagggcgg gttggcgtgg gccgttccag
    10381 cgggcgcttt aaaccgcggg tcgtggtcgc catcgcgctg gacgatcagc agcgcatcgt
    10441 cgacaccttg tttatgaaag gactgaccgt cttcgcccga ccgcaaaaaa ttcccgcaat
    10501 taccggtatg catgcgggtg atttacagcc cgatgtgatc tttccccatg atccactatc
    10561 acagaatgct ctatcattgg cgcttaaact gaaacgtgga taatttcgtt gtgaatgtta
    10621 cttgcttgcg aagttatcat tttgaaacct aaatcaggta atcacgccca tgaaacctcg
    10681 tcagcgtcag gccgccattc tggagtatct gcaaaagcag ggtaaatgct cggttgaaga
    10741 attggcgcaa tactttgaca ccacaggcac aaccattcgc aaagatctgg tcattctgga
    10801 acatgccgga accgtcattc gtacttatgg cggagtggtg ttgaataaag aggaatccga
    10861 tccgcctatc gatcataaaa cactcatcaa cacccacaag aaagagctga ttgcagaagc
    10921 tgccgttagt tttatccatg atggcgattc gatcattctt gatgctggca gtaccgtttt
    10981 gcagatggtt cccctgctct cgcgctttaa taacatcacg gtgatgacca acagcctgca
    11041 tatcgtcaat gcgctatccg aactggataa cgaacaaact atcctgatgc caggcggaac
    11101 gtttcgtaaa aaatcggcct catttcacgg ccagctggca gagaatgcct tcgaacattt
    11161 cagtttcgat aaattgttta tgggcaccga cggcatcgat ctcaatgcgg gcgtaaccac
    11221 cttcaacgag gtttataccg tcagtaaggc aatgtgcaat gccgcgcgcg aagtgatttt
    11281 gatggcggac tcatcaaagt ttggccgtaa aagccccaac gtagtttgca gtcttgaaag
    11341 cgtcgataag ctgattaccg acgcaggtat cgatccggcg tttcgtcagg cgctggaaga
    11401 gaaagggatc gatgtgatca taaccggaga gagcaatgag tgaagcacta ctgaacgcgg
    11461 gacgtcagac gttaatgctg gagttgcagg aagcaagccg tttaccggaa cgtctgggcg
    11521 atgattttgt tcgcgccgcc aatatcatcc tgcactgcga aggcaaagtg gtggtttcgg
    11581 gaattggcaa atcgggccac attggtaaga aaatcgccgc aacacttgcc agtaccggca
    11641 caccggcttt ttttgtccat ccggcagaag cactgcacgg cgatctgggg atgattgaaa
    11701 gccgcgatgt gatgctgttt atctcttact ccggtggcgc gaaggaactg gatctgatta
    11761 ttccgcgtct ggaagataaa tctatcgcgc tgctggcgat gaccggcaaa ccgacgtcac
    11821 cgctgggcct ggcggcaaaa gcggtgctgg atatctccgt agaacgcgaa gcctgcccga
    11881 tgcaccttgc gccgacctcc agcaccgtca ataccctgat gatgggtgac gcgctggcga
    11941 tggcggtcat gcaggcgcgc ggatttaatg aagaagattt tgcccgctcc cacccagccg
    12001 gggcactggg cgctcgcttg ctgaataaag tgcatcatct gatgcgccgt gacgatgcca
    12061 tcccacaggt ggcgttaacc gccagcgtga tggatgcgat gctggaactc agccgcaccg
    12121 gtctggggct ggtggcggta tgtgacgctc aacaacaggt acaaggcgtc tttaccgacg
    12181 gcgatttacg tcgctggctg gtaggcggca gcgcactcac cacgccagtt aatgaagcga
    12241 tgacgacagg cggcaccacg ttacaggcgc aaagtcgcgc catcgacgcc aaagagatcc
    12301 tgatgaagcg caaaatcact gccgcaccgg tggtggatga aaacggcaaa ctcaccggcg
    12361 caattaacct gcaggatttc tatcaggccg ggattattta atccttcatt cccagacgtt
    12421 tcgccagccg atgcaggttg gcgacgtcgg tttccagcat ccgcgcgcag gcagcccagt
    12481 tatgatgatt ttgtgccagt gcctggcgaa tagtttcacg ctggaacgct tctgtcgctt
    12541 cacgcaggtt ttgcttaaca acgggcaccg ccgccgcttc tggcggcggc aacgtcacct
    12601 caggaaaagc aaaatgttgc gcctcaagaa tcacttcatc gccgttgcgg gtggctctcg
    12661 ccagcactac cgcccgatga atagcatgtt ccagttcgcg cacgttcccc ggaaaacgat
    12721 agtgttgcag taaatttcgc gctccggcac ttaataccac gcgggagagc cccagccgca
    12781 aacgacactg ttcgcagaaa taccccgcca gcagaatgac atcatcgccc cgctcacgca
    12841 gcggcggcac cgaaagtgga aacacgctca ggcgatgaaa caagtcagcg cgaaatcgcc
    12901 ctgccagcac ctcttcgcgt aagtcgcggt tagtcgccgc cagcacgcgc acatcgaccc
    12961 gcaaactacg gtcatcgcca acgcgctgaa tatcgccata ctgcaacacc ctcagcagct
    13021 tggcctgcaa tgccaacgac aactcgccga tctcatccag aaacagagtg ccgttatccg
    13081 ccatttcaaa cttcccgctg cggttactga tagcgccagt aaacgctcct ttcacatgcc
    13141 cgaacaactc actttccgcc acactttccg gcagtgcagc acagttgaga tagaccagcg
    13201 gattcaccgc ccgtggcgag gcttcatgaa tcgctttcgc caccagctcc ttaccggttc
    13261 ccgtctcacc gctgatcagg acgttgagat cggacgccgc cacaatctca atctcttttt
    13321 tcagttgcgt catgccaggg gacaagccaa tcatctgcgt ctgtttcacc gcttcaaacg
    13381 gcgtggcatc gcttggcatc atattctggc tttccagttg ttcaatcagc aacgcattgc
    13441 ttaacgctcc cgccgccagc gcagcaatca gccgtagctc ttcgtcgctg aaaacatcga
    13501 actgatcggg ctgcatcccg tcgagcgtca gtgcgccgat caggttttgc ccggcaaaca
    13561 atggcagacc aacgcaggcg tgaaccttca gactctcctg cccaggaatc aaaccgtcat
    13621 agggatcggg caattcgctg tctgcgggaa agcgcaccac atccccggcg cgggcaatcg
    13681 cttccagccg tggatgccct tccagcgcaa agcgtctacc gagtacaccc tttgccagac
    13741 cgtcgatggc aagcggaata aactgccgcg aatcgtaacg tagcaacgca gacgcatcgc
    13801 actccagcac ctgacgtagc gtggtgatca ggcgctgaaa acgatcctga tgaccaatcc
    13861 cacgctgcaa ttcgatggcg atattcgcca gcacatcaac ggaaaaactc atctttgcct
    13921 cactgtcaat ttgactatag atattgtcat atcgaccatt tgattgatag tcattttgac
    13981 tactcattaa tgagcataat tttatttata gagtaaaaac aatcagataa aaaactggca
    14041 cgtaatctgc aattagcaag acatcttttt agaacacgct gaataaattg aggttgctat
    14101 gtctattgtg gtgaaaaata acattcattg ggttggtcaa cgtgactggg aagtgcgtga
    14161 ttttcacggt acggaatata aaacgctgcg cggcagcagt tacaacagct acctcatccg
    14221 cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa ttcagccgcg aatttgtgca
    14281 gaacctgcgt aatgaaatcg atctggcgga tatcgattac atcgtgatta accatgcaga
    14341 agaggaccac gctggggcgc tgaccgaact gatggcgcaa attcccgata cgccgattta
    14401 ctgtactgcc aacgctatcg actcgataaa tggtcatcac catcatccgg agtggaattt
    14461 taatgtggtg aaaactggtg acacgctgga tatcggcaac ggcaaacagc tcatttttgt
    14521 cgaaacgcca atgctgcact ggccggacag catgatgact tacctgacag gcgacgcggt
    14581 gctgttcagt aacgatgctt tcggtcagca ctactgcgac gagcatctgt tcaacgatga
    14641 agtggatcag acggagcttt tcgagcagtg ccagcgttac tacgccaata tcctgacgcc
    14701 gttcagccgc ctggtaacac cgaaaattac cgagatcctg ggctttaact taccagtcga
    14761 tatgatagcg acctctcacg gcgtggtatg gcgcgataac ccgacgcaaa ttgtcgagct
    14821 gtacctgaaa tgggcggctg attatcagga agacagaatc accattttct acgacaccat
    14881 gtcgaataac acccgcatga tggctgacgc tatcgcccag gggattgcgg aaaccgaccc
    14941 acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat aaaaacgaaa tcctgaccaa
    15001 tgtcttccgc tcaaaaggcg tgctggtcgg cacttcgacg atgaataacg tgatgatgcc
    15061 gaaaatcgcc gggctggtgg aggagatgac tggtttacgc ttccgtaaca aacgcgccag
    15121 tgctttcggc tctcacggct ggagcggcgg tgcggtggat cgtctttcca cgcgcctgca
    15181 ggatgcgggt ttcgaaatgt cgcttagcct gaaagcgaaa tggcgaccag accaggacgc
    15241 tctggagtta tgccgtgaac acggtcgcga aatcgcccgt cagtgggcgc tcgcgccgct
    15301 gccgcagagc acggtgaata aggtagtcaa agaagaaacc tctgccacca cgacggctga
    15361 cctcggccca cggatgcagt gcagcgtctg ccagtggatt tacgatccgg caaaaggcga
    15421 gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa gtcccggata acttcctctg
    15481 cccggaatgc ttcctcggca aagacgtctt tgaagaactg gcatcggagg caaaatgagt
    15541 aacggcattg tgatcatcgg ttcgggcttc gccgcccgcc aactggtgaa aaatattcgc
    15601 aaacaggacg ccactattcc attaaccctg attgccgccg acagcatgga tgagtacaac
    15661 aaacctgacc tcagccatgt tatcagtcag gggcaacgtg ccgatgacct tacccgccag
    15721 acggcgggtg aatttgccga gcagtttaat ctgcacctgt ttccacaaac ctgggtgacg
    15781 gatatcgatg ccgaagcccg tgtggtgaaa agccagaata atcagtggca atacgacaag
    15841 ctagtactgg caaccggtgc cagtgccttt gtcccgcctg tgcctgggcg tgagttaatg
    15901 ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa cgcaactgcg ggatgcccga
    15961 cgcgtgttga ttgttggcgg tggtttgatt ggtagcgaac tggcgatgga tttttgtcgt
    16021 gcaggcaaag cggtcacgct aatcgacaac gctgccagta ttctggcgtc gttaatgcca
    16081 ccggaagtaa gcagccgctt gcagcatcgg ttgacggaga tgggcgttca tctgctgttg
    16141 aaatctcagt tacaggggct ggaaaaaacg gattctggca ttctggcaac gctggaccac
    16201 cagcgtagta tcgaagtgga tgcggtaatt gccgccaccg gactgcgccc ggaaacagcc
    16261 ctggcacgac gcgccgggct gacgattaat cgcggcgttt gcgtggatag ttatctgcaa
    16321 accagtaatg acgatattta tgcgctgggc gattacgcgg aaatcaacgg tcaggtactg
    16381 ccattcctgc agccgattca acttagcgcg atggtgctgg caaaaaatct tctcggcaat
    16441 aacacgccgc tgaaactacc gacgatgctg gtgaaaatca aaacgccgga attaccgctg
    16501 catctggcag gcgaaaccca gcgtcaggat ttacgctggc aaattaatac cgaacgccag
    16561 ggaatggtgg ctcgcggtgt tgacgatgct gaccagcttc gcgcctttgt ggtcagtgag
    16621 gatcggatga aagaggcatt tggattgttg aaaacattgc cgatgtaggt gggctactgt
    16681 gcctaaaatg tcggatgcga cgctagcgcg tcttatccga cctacgggga cgcatgtgta
    16741 ggccggataa ggcgtttacg ccgcatccgg caatggtgtc caaatgcaac acgttttatc
    16801 cgttctggac ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc cccagagaca
    16861 aaccgccatc acccgccggt aaactctgtg gaaagagcaa tgtgaaatca gcgagataat
    16921 gcgccagacg tgcacgcagc aaacggttat gaataacccc gccgctaaat accagcgtag
    16981 tgataccacg catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat
    17041 catgaaacgc ccacgcgcgt tgattaacct gtgcctgcca gttcagccac tgctgccaga
    17101 aagcggcgag atccagttga ttgtccacca gcggcatcgt caccggatgc gtcactccgt
    17161 ggcatgaggc tgcgagcgcc tccagagcac aagccgcttc accttcataa cttaacgtgg
    17221 ctggcgcaca gcccagtgcc gccgccactg catcgaacaa ccgcccacac gatgacgcca
    17281 gcggcgcgtt aattccacgc tcaatggccc gcgccagcac gctccagttt tgctgttgca
    17341 cacttgctgt ttcagggtaa ttctgccact ccggcacaaa gcgcaggcac tgcgccagca
    17401 ggtttcgcca cggctgcttc gctgccaaat cgccacccgg aagcgccact gcaggcaagc
    17461 cgcccaggtg ctcacattcg cgatagttca cccgcaggca ctcgccgccc cacaaagcgc
    17521 cgttctcccc cataccgata ccgtcgagcg tcaaagcaat gacatcaccg ccatccagcg
    17581 gccactgatg ctctgccaga cacgccgctg catgggcatg atgatgcagt accgtttgcg
    17641 tcggcagatt catttcacgc gcccactggc tggagacata gcccggatgc gcgtcatgca
    17701 caacgtattt cggggtaaaa tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact
    17761 gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc gcttgttcac
    17821 cgcgcaccag gcagaaggta tttttcagat ccgctccgag acacagcaca ggcggaacat
    17881 ttttaaagcc cggaggcaaa gccagcgcat ccggcacata cccccgcgaa cggcgcagca
    17941 tttcgccgct ttcgcgcacc accgaatcat ccatccgctg cacgatgtcg cggttatgta
    18001 tcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg ctgatagctg
    18061 gtggtttacc gctcaggttg ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca
    18121 gcaaatgctg gagcgggttc gcaggcaaca ttaccccgac ttcgttaagg tcaggggcga
    18181 tatcatcaca aagctcagga acgtattttt tatccaccag cacaatcggc gcggcgggcg
    18241 tggtaagcaa ctggcgcgca gcgtctggta aaccgtcagc cactggcaac atgaccgcca
    18301 gcggtttcgc cgggcgatgt ttgcgcgccc gaagtgtcgc caccgcgtta ctgttacgtg
    18361 catcgcaggc aagatgaaat ccgccaatcc ctttgatggc gacaatgttg cccattttta
    18421 actgtgcgat agccgcctgt aatgacgcct cttgttctgc atgttcacca tgacttaccc
    18481 attcaagatg cgggccacac tccgggcagg ccaccggctg ggcgtggaag cgacgatcga
    18541 gcgggtcgcg gtactctttg tcacaagccg gacacaacgg aaacgccgcc atcacggtaa
    18601 acgggcggtc gtaaggcatg gcgcgaataa tggtgaaacg caggccgcag tgggtacagt
    18661 tgataaacgg ataacgataa cgccgttcgc ctggggtatt catttcggca aggcaagcag
    18721 ggcaagtagc ggcatccggg acaatttgcg tattcatggc acctcccgcg ctctggcgga
    18781 tggtgaactc agtgggcagt tgtgaccaga tgtacggctc acgctcgacg ctatcaatac
    18841 gcgccagcgg cgggcagtgc tgatgcaatt gaacaagaaa cgtttccggg tcttcccgca
    18901 gccggacttc tacgccatcg ccgtcattac agacatcgcc gtgaagattt aattgctgtg
    18961 ccagctgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga atacgcagtt
    19021 ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga attgcgtaac
    19081 ccgcccagcc ggacacgaca gcgtcgcatc cggcagtcac aggtcggcga taccgcccgc
    19141 tccgtattct acgaatattt ccgggaattc ctttgatgcc agaacagttc tgtaagattt
    19201 ttagaacatc agcgccgtac ggcggcgttt ttctgcactc agttgttcaa gtttattacg
    19261 atcgacacaa atcagcgcat gagtcgggca agccgccata cacgccgggc cgtcttcacg
    19321 atggttgcac aggtcacatt tattggcttc ggctttgtca gcccgtacat tcagccccgc
    19381 gccgctgttg cgaatcaccg gacgtaccac cacttccatc gcgccatacg ggcaagccac
    19441 aacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca tgaacaaacc ctttatcacg
    19501 gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact gacggcaaac
    19561 tgtcgccgtg gaaatgttca cacctttaat gacatggata cgcggtaaaa atgtttccgg
    19621 ggtcagcgat gcacagtcct gattttcctg atgagaaacc acgcacgcca cttcacaggt
    19681 acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca aattctccag
    19741 caatgacagt taatgcgccg atacattcac aaatcatgcc agtttttaat tcactgttat
    19801 ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc atcgacacta gtgacgatga
    19861 catgtgatga caatgtttat cgcgaaagag caatgagtga gtcgcggtgg atcagttttc
    19921 cgctgaaggt tttcggcggt gagaaatccc cgccatcgag cataaaaatc agccgtccaa
    19981 taatttcctg aatcatctca gtcaccggaa tttttacgct ggagagcgcc ggaacgatat
    20041 agggggcaat tgcgatatca tcgaatccga taactgacac ctgctctggc accgctacgc
    20101 cgcgctcgtg caacgctttc atcgcaccta tcgccatatc gtcgttactg gcaactaacg
    20161 cgctaaattt agccccacgt tcgagcaacg tttctacccc ttcggccccg ctggcgggcg
    20221 tccatttacc gttagcgata agtttttcat tgagcgcaat accatgctgc gccagcgcgt
    20281 ctttataccc agcaagacgt tcaatgctgg tgggggaatc catcgagccg gtaaggaaag
    20341 caatctcctg atgtccggcg tttatcaact ctgccacggc attaaagctg gtctgtttat
    20401 gatcgcacca gacgctatgg ctgctgtttt tgcgcaggcg gcgattaagc accattatcg
    20461 gctgactgtg cgcgtcaatg atgtcatcga tctcatccac gcttaaaaaa cgcgggtaaa
    20521 tcataatggc gtcgcagcgc aaatccagca gatactgaat cgcctggcgc tcttcttcgg
    20581 cgctgtgttt accatctgcc aatagcaact gccgcccttt ctcttccgcc attcgcgcgg
    20641 catgaaagag taattcacta aaataaatgc cgtggtaaag cgtgttggtc actaccagcc
    20701 ccagcgtctg agtactcttc gccgacagat tgcgcgccag caagtttggg cggtaaccgc
    20761 tctcttctac cgcctgaaac acgcgatctt tagtctcctg gctgacgtag ccattacctg
    20821 aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg cttcgccact tccagcatcg
    20881 tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt gtactgcacc gtttgccgat
    20941 tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg gtttcacaaa tataaaaaat
    21001 gaataattaa ctctcttgct tatttagtga caactattca tgattttgtg aaacgggttt
    21061 cctgattccg tttcagcatc ggcatttttc cgttacgtcg actgataaca actacatcta
    21121 ccctactgat aacaggataa aatccgatgg ccaaaaatta tgcggcgctg gcacgctcgg
    21181 tgatagcggc actgggcggc gttgataaca tctcggcggt cacgcactgt atgacgcgtt
    21241 tgcgctttgt tatcaaagat gaccagctta tcgacagccc gacgttaaaa accatccccg
    21301 gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt gattatcggc aataccgttt
    21361 cacaagcctt tcaggaagtc gtcagcctgc tgccgggaga tatgcagccc gcacagcccg
    21421 tgggtaaacc caaactcacg ctgcgtcgca ttggtgcggg gatcctcgat gcgctgatcg
    21481 gcaccatgtc accgctgatc ccggcgatta tcggcggatc gatggtcaaa ctgctggcga
    21541 tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc gaccttaacc attctgaatg
    21601 tgattggtga cggtgctttc ttcttcctgc cgctgatggt cgcggcatct gccgccatca
    21661 aatttaaaac caatatgtcg ctggcgattg ccatcgcggg tgtgctggta catccgagtt
    21721 ttattgaact gatggcgaaa gcggcctagg gtgaacatgt cgaatttgcc ctgattccgg
    21781 tcaccgcggt gaaatacacc tacacagtga tcccggcgct ggtgatgacc tggtgcctgt
    21841 catatatcga acgctgggtg gatcgcatta caccggcggt gaccaaaaac ttcctcaagc
    21901 caatgctgat tgtgttgatt gccgcaccgc tggcaatcct gctgattggc ccgattggta
    21961 tctggatcgg tagcgccatt tcggcgctgg tttacaccat tcatggttat ctgggctggc
    22021 tttcagtcgc cattatgggc gcgctgtggc ctctgctggt aatgaccggg atgcaccgcg
    22081 tctttacgcc aaccatcatt cagaccattg ccgaaaccgg caaagaaggg atggtcatgc
    22141 cgtcagagat cggcgctaac ctgtcgctgg gcggttcatc actggcggtg gcgtggaaaa
    22201 cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc ggcatcagcc attatggcgg
    22261 ggatttccga accggcgtta tactgcgtgg cgatccgcct gaaacgtccg cttatcgcca
    22321 gtcttatcag cggttttatt tgcggcgcgg ttgccggtat ggcggggctt gccagccact
    22381 caatggcagc gccggggcta tttaccagcg tgcaattctt cgatccggcg aatccaatga
    22441 gcatcgtctg ggtgttcgca gtcatggcgc tggcggtggt gctgtcgttt atcctcacac
    22501 tgttgctcgg ctttgaggat attcctgttg aggaagcggc tgcccaggcg cgaaagcatc
    22561 agagcgtaca accgaccgtc gccaaagaag taagtcttaa ttgaggatga aaatgtcagt
    22621 atttccagaa ggttttttat ggggcggcgc gcttgccgcc aaccagtctg aaggtgcgtt
    22681 ccgtgaaggc ggaaaagggc tgaccaccgt cgatatgatc ccacacggcg agcatcgaat
    22741 ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc gatgacgagt tttatcccag
    22801 ccatgaggcg acggattttt atcatcgtta taaagaagat atcgccctga tggcagagat
    22861 gggattcaag gttttccgta cctcaattgc ctggagccgt ctctttccgc agggcgatga
    22921 aatcacgccc aatcagcagg gcattgcttt ttatcgttct gtctttgaag agtgtaaaaa
    22981 gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat gtgccgatgc atctggtcac
    23041 cgaatatggc tcttggcgta accgcaagct ggtggagttt ttcagccgct acgccagaac
    23101 ctgctttgaa gcatttgatg gtctggtgaa atactggcta accttcaatg aaatcaacat
    23161 tatgttgcat agcccgttct ccggcgcggg tctggtgttt gaagaaggtg aaaatcagga
    23221 tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc agtgcgctag ccgccaaaat
    23281 cgcccatgag gttaacccgc aaaatcaggt gggctgtatg ctggcgggcg gcaacttcta
    23341 cccttacagc tgcaaaccgg aagatgtctg ggcggcgctg gagaaagatc gggaaaacct
    23401 gttttttatc gatgtgcagg cgcgcggcgc gtatccggct tactctgccc gcgtatttcg
    23461 cgaaaaaggg gtaaccatag ataaagcgct gggcgatgat gagatcctga aaaacaccgt
    23521 cgattttgtc tctttcagct attacgcctc gcgctgcgcc tcggcggaga tgaacgccaa
    23581 caacagcagt gcggcgaacg tggtgaaatc gctgcgtaac ccgtatctgc aagtgagcga
    23641 ctggggctgg ggaattgatc cactcggtct gcgtatcacc atgaatatga tgtacgatcg
    23701 ttatcagaag ccgctgtttc tggtggaaaa cggcctcggc gcaaaagatg aacttgctgc
    23761 caatggcgag attaacgatg actatcgcat cagctattta cgcgaacata tccgcgcaat
    23821 gggcgaagcg attgcagatg gcattccgct gatgggctac accacatggg gctgtattga
    23881 tttagtttcc gcctctacgg gtgaaatgag caaacgctac ggttttgtct acgtagaccg
    23941 tgacgacgca ggcaacggca cgctgacgcg cacacgtaag aaatcgttct ggtggtataa
    24001 aaaagtgatt gccagtaatg gggaagattt agagtaagta acagtgccgg atgcggcgtg
    24061 aacgccttat ccggcctaca tgtcgcacga accaggaagg cccgataagc gcatcgggca
    24121 atttagcatt tggcatcaga gctggttcac cccatcaaga acatccctgt cctgattcct
    24181 taatgaaaaa ctactcttct tccaccgcta actgcgcgaa gccgccattc ccttcccagc
    24241 cttccagtcg ttgataaacg gtttctaccg catctttaat cggctgggtc atcgggtagt
    24301 aaaagccgac gatatccggc tgtatgccga ggaaaatcac ttcgccaata tcttctttca
    24361 actggtcgat aagatagttc agaggcatgt tatgggtagt catcataaac atctcggcga
    24421 tatcatccgg gtcgatgatg cggatctcgc cggggtttag ccccatatcc gtggcgtcga
    24481 caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac gatgtcgttt tccggtgcac
    24541 taccgccgtc aatcaccacc cagttacctt tcggcgcggc ggcgcacttt tccgccagca
    24601 gcggacctgc gccatcatcg cccatcatgc tattgccaac acagagtaaa acgtcagtca
    24661 cgcaatctcc tcaccatcaa atagatggcg ttttcctgat gaatatcatg cagcatactt
    24721 aacagcattg tactccattc ctgctgctgc gaagtttgcg ctgcccgggc tttatcgaat
    24781 gcgttggcca gcatcggcac atggttaatg tcgatgacga tctcaccgta cttcggtacg
    24841 ccttccattt tgcggcgggc ttcactgccc gcctccagag tggcaatcca tgccagatat
    24901 tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga taaccccaag gtggtgacca
    24961 atcgccaggc tgtaatagac cacctgctgc gcctcggcgg gcgtggcatc gttctcatca
    25021 ataaatttac ggctcagttg actgaacacc accttttcac tcatcggata cgcacctctt
    25081 ctacaacatg attcagatgg ctgacaatct cgttcagacg cggatcgttt tccgcttcca
    25141 gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt caggaagtca tcggcaatct
    25201 gacgaccgta acgataaccc gccaggcggc gtgcttcgcg atccactttc acgcgcagct
    25261 gctgcaccat atcgccatgc aggatctccg ccggttgttc atccagttca cccggcccac
    25321 gggcgtgaat tttctgctcc agcaggccga gcgccattgc aaagccgtac agcgtggcgg
    25381 caggcgttgg cgggcagcca gggatataaa catccacagg gacaatttta tccgtaccgc
    25441 cccacacgca gtagagatcg tggaagatcc cgccgctgtt accgcaggca ccgtaggaga
    25501 tacaaatttt cgggtccggc gcggactgcc acgcacgcag cgcaggggat cgcattgcac
    25561 gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg cggtgaagga acgactttaa
    25621 tgccgaagcg ttctgcatca aacagcggcg ataaagtggc gaaaatttcg atttcgcagc
    25681 cgttgcagcc gccgcagtcc acgcggtaaa cataggcaga acgtttgatt tttttcagta
    25741 acgacgcctt catgctggcg atggattcat ccaccgtcat cgggaccgga atgccgttgg
    25801 cgtcacgggg gcctaataaa ttgctcatca gatggcctct ttcatatggc gagtcagttc
    25861 aatacggtcg gacggcacca ggcatttctg gcgcttacat tccgggcaag tctcaaagct
    25921 ttcgcggtgg ttttccgcgc ggctgtcgcc gttgtgctta agcagcgcaa tggcgtagtc
    25981 gatctctttc tggacggcga aaggacgatt gcagacgcga cagttgcaca gcgcgaagcg
    26041 agactgttgc aggaagtctt ctttcttcca caccgccagt tcgtactctt gcgacagctt
    26101 aatcgccgcc gtcgggcaaa cttcttcgca gcgtccacag aagatgcagc gcccgagatt
    26161 aaactcccag gccagctctc ctgtggcgag gtcagtttca accgttaagg cgtttgacgg
    26221 gcaggcattg acgcaggccg cgcagccgat gcactgctgc gggttctgct ctggcttacc
    26281 acggaagttt ttatcaaccg caatcggctc cagcggataa gacgaggtcg ccgtgccggt
    26341 tttgataact tttttgataa aggtaaacat ggcgattcct tatttcagcg gcgagttttt
    26401 acgctcaatg ctgtagcgct cgagttcttt gtacggcacc actttgctct tcttcttacg
    26461 tacatcgacc acagtcatgc ggtcggtaca ggagtagcaa gggtccaggc taccgataat
    26521 cagcggcgca tcggaaacag tgttgccacg cagcatgtag cgcagggtcg gccagttcgc
    26581 gtaggtcgcg gcacggcagc gccagcggta cagcttctgg ttgtcgccag tcatgctcca
    26641 gtggatatca tcaccgcgcg gcgcttcggc aaagcccagc gcgaaacggt gcggaatgta
    26701 ggtaaagcct tccaccatca gcgggccacc cggcaggtta tccagaccgt agtcgatcat
    26761 gttcagcgcg gtatagactt cgttgatacg cactttcaga cgcgaaataa cgtcgcagcc
    26821 ctgctcgctg tggacttcca ctggcagcag gccataacca acaaacgggt gatcagcgcg
    26881 ggtatcgcga gcgtgtccgc tggcgcggac catcgggcca acgttactga aatcgcgggc
    26941 aatttccggg tccagacgac caatgccgac ggtgcgctgt tccatgttcg gtgtgctcag
    27001 cagcacatcc accagctcct gcacttcacg acgcatctgt tgcgccagct ggtgggtctg
    27061 gatcatgtca tctttcaaca gatcgcgacg aatcccgccg atcaggttca ggccgtaggt
    27121 tttacgcgca ccggtgagga tctcggccat tttcatggag gtttcacgca cgcggaagaa
    27181 ctgcataaag ccgtagtcga agccggtaaa gtggcaggcg aggccgaggt tgagcagatg
    27241 cgagtgcagg cgttccacct ccagcagaat ggcgcggatc atctgcgcac gttctggcac
    27301 ctgaataccc atcgcgtttt ccaccgacgt ggtgtaggcg gtgctgtggg cggagccgca
    27361 gatcccgcac acacggtcag agaggaaggt cacttcgtta taacccatgc gggtttccgc
    27421 cagtttttcc ataccgcgat ggacatagaa caagcggtag tctgcgtcga taatgttttc
    27481 gccatcgacg aacagacgga agtggcccgg ttcgtcagaa gtgacgtgca gcggaccaat
    27541 cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg aactcgtagg tttcagcacc
    27601 ggtggtcggt gccggacgct gacgataatc catgctgtct ttacgcagcg gataaagttc
    27661 atccggccag tcatccggca gcaccagacg gcgttcatcc ggcagaccaa ccggaatcaa
    27721 accgtacata tcgcgcactt cgcgctcgcc ccacaccgcc gccggaacgc gcggcgtcac
    27781 ggacggatac tccggtttgt tggcatcaac ttcgacgcga accgttaccc agcacttagt
    27841 ccccttctcc atcgacagca cgtaataaac ggcgtaatga ccattcagtt tgcgttcgtc
    27901 gttaccaaaa agcaccgaca gccagccccc ctgcttgtag tagagaaact ccaccacttc
    27961 cggcagatag ttcaccttta cggtgacagt cagctgatct ttggtctgcc aggcgtggtc
    28021 cagcacgacg cccggaaatg cctcattcag cgcggcgaga taatgttgac ctaatttttc
    28081 ttcagacatg ctcaaactct ctttaatcac gccgccagca aggagacgaa cgctaaaaat
    28141 gcaaagccaa acccagccca ggtaatgcgc ggagtaatat caagacgcag acgggccatg
    28201 ctgttttcga acagcgcgat aaccaggacg ccgaccacca gttttacgat ggcaatcacc
    28261 agcgccagca gcagtccacc ggcggtgaag gtttccattt gtccccacgg aataaacacc
    28321 ccgacgaaca tctgcaacac caccagctgt ttcaggctga taccccattt catgacgcca
    28381 aagccgctgc cgctatactc agagagcgga ccttcctgca actcctgctc ggcttccgcc
    28441 aggtcgaacg gcagtttgcc catttcgata aaagtggcga acgcacaggc gcagagcgcc
    28501 agcaccagcg ggatgctttg gctcaacggc cagtgataaa cggtgtcggt aatgttgctg
    28561 atgttggtgg aaccggcaac ctgtgcggca acccacagac caagcagcaa catcggttcg
    28621 accagcacgc caagcatcgc ttcacggctc gcaccgatag cggtaaacgg gctaccggta
    28681 tccagaccgg aaatggcaaa gaagaaacgc gcgatggcga agagatacag taaggtgatc
    28741 aaatcgccca gttgcggcag cggagaaccg acggtcacca ccggcagcgc agtagcgata
    28801 gtcagcatga cgcccaccat cacatacggc gtcaggcgga acacccagcc agaggcatcc
    28861 gggccaacgc tctggcggcc cagcagtttg ataatgtcgc gatactcctg caacacgccc
    28921 ggcccgcgac ggttatgcag acgggcgcgc gccacgcggg taataccgga gagcagcggc
    28981 gcaacggcaa ataacaccag cgcctgaatt aacggatata aaacactcat tctcaggctc
    29041 ctcgtgaaac aataatcacc accagtaccg ccagttcaac cagcgccatc cggcggaaca
    29101 gcaacgcact accttcgcac tgccagcccg gcaccagcga caccggattc agccatttgc
    29161 gcagtttcag cactggcgca aacgcctgtt taaccggcat ggcaaaaccg tgagcggtaa
    29221 tcaccattga tttttcatgg tcgtagccgc acacccaggc cgcaccgcgg gaacgtgacg
    29281 gcaggcggtc gcctttgcaa atcgccataa tgatgaatgg cagcagcggg caggcaatca
    29341 gcagcaatgt gatcattggt tgagaaacgg tggtgttagc aggctccaga ggcagaggta
    29401 cggcagcaga gagcatcggc agcagccacg gcgcagcaac accgccaatt acgcagcaaa
    29461 tcgccagtgc cactacgctt acgctcatca ggagcggcgc acaggtggcg ttttcggctt
    29521 ctttggtacg cggcgcgccg aggaacgtga cgccatagac tttcgccata cacatcaccg
    29581 ccagcgcacc ggtaattgcc agacccacag cgagcagcgg ccccagcaga cgggcaacaa
    29641 acgcgccact attgctcagt ttgaaaaagg attgatagat aacccattcc ccggcaaacc
    29701 cgttcagcgg cggcagcgca gccattgcca tcaacccgac taacatggcg atggagataa
    29761 ccggcatttt cttgccaata ccaccgagtt tttcgatatc gcgatgaccg gtacggaacc
    29821 agacgctccc cgccccgagg aacagtacgc ttttgaacag gctatggtta agcagatggt
    29881 acagaccacc gaccaggcca agagcaatca gcgccggttg ttcgagcgcg atacccgtta
    29941 cgccagcgcc cagccccagc cccagcccca gccccagccc cagccccagc cccagcagga
    30001 taatgccgat attttccagg gtgtggtaag ccagcaggcg ctggatgttg tgctccatca
    30061 gcgcatacag accaccgaca aacgcggtga tcatgccgag caccagcagc acgatccccc
    30121 accacagcgg tgcattaccg cccagcagtg acagggttaa aatgcccagc aggccaattt
    30181 tcatgactac cgtagaaaac aacgcggcag ctggcgcaga ggcgttagca tgtgcctgcg
    30241 gcacccagcc gtgcagcgga ataatcccgg cgagcaggcc aaagccaatc acaccgagca
    30301 gccagatatc ggaaccgagc ggcagctgtt gcatacgcat atccagcagg cgcagatcca
    30361 gcgtgccata acgctgccac agcagccagc aagcaagcaa tcgccagcag cagagtgcca
    30421 agacgcccca gcgcaaacca cagtttgccc tctttgctgt tgctggtgag gaacaccgca
    30481 cacaaggcca tgacttcggc cattaccacg aacatgccga ggttgctggc gatgacggcg
    30541 cagacggcgg cagccatcaa catattgatc tgcaagccgt tgcacttcac ctgcgcgtgg
    30601 cgatgccagt caatgttgta gaggctgaca aacagaccgc acagaccgag cgtaatcagc
    30661 caaatcgcgt taagcgggga gatttgcaca tcgtagctta ccagcgacag cgcaccgctc
    30721 acgccaaccg cgccagtcag cacagtgaag cccgcggctg ccgtatacag gctaccaacc
    30781 gcgccgccaa ttccggctat ccagccactt agcgtctttt gaaaagaaaa gagaaatgcc
    30841 agaacagcgg cggcgacaaa ccacaccacg ccgctattga tcagggaaat tgcgctcatt
    30901 tagcctctcc actttgagcc tgctgaaaca aggtgagatc gccaaagtcc gtattaaaag
    30961 tcagctcacg cttacgtttg ctgacgcggg cgatatcggt gttatccacc agatgcaggg
    31021 ctttggtcgg gcacatccgc acgcaggccg gaccttgttc atcaaagcta caaaggtcac
    31081 atttgacggc gatcgcgcga atacccggca cccagtcaag cagcgtactg atacgcgccg
    31141 gagcaggcgg tgccggtggc gctttcgggg tattggcatt tgcaggaata tccagcggac
    31201 ggctgccgga aaattcaatt gcgccaaacg ggcaggcgat gccgcacagc tggcagctta
    31261 cgcacaggct ttcattcaac tgcacggccc catcaacgcg ggtgatgacg ttaaccgggc
    31321 agaccaccgc gcagggtgca tcttcacagt ggtgacagag ctgcggcgca gattcttttt
    31381 cattcagcat cactctcagg cgcggcattg attgcaggcc gtgctggcga tgcgtctctg
    31441 aacaggctgc ctcacaagtg tggcagccga tacagagcgt ggagtcagca attacaaaac
    31501 gattcaccag gcattcctca ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt
    31561 cattttcgac actcatcgac acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc
    31621 aaagcctcag ctcatgctgc cgggctttgt ccctttacca gttggcttaa attcaccggc
    31681 acattgtttt caatggcggt gtataagctg tcgtaaacgc cagcgatttt ttcgagatag
    31741 tgttccagaa cctgttcgcg atcgcggatg ttgaccttgc catcaatcat gccgtcaatg
    31801 ctcagttgcg cctgcgcaat ccgctgtttg tcttcgccct cttttgtttc gacgtaatac
    31861 tcgatggtgt ggctgttaaa gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa
    31921 aggacgaaac agagttcttt gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc
    31981 gataacgtga tcccctgaac cagcgaattg cagtagatgt gccactggtc ctgtagacga
    32041 cgatgccgct gtgcgatgta atcggctttc tcgcttattt cccaaatagt cattgtcagg
    32101 ttacccgttt aacagagatg ccagctttaa gcattttctg tgccaatttt taatttattg
    32161 ttattaaaac gatttttaag ctaaagatga atttcgtcgc cgtgtcgacg tgtcatttcg
    32221 acatcatcga cattattcac cgcagggata atcaacactg gcacaattat tgcttgtagc
    32281 tggcaatagt taatgggagg cgatatgcac gcaataaccc tctgccaacg ggcactggaa
    32341 ttgatcgaac agcaggccgc aaaacacggc gcaaaacgcg taactggggt ctggctcaaa
    32401 attggcgcat tttcttgcgt cgaaaccagc tctcttgcct tttgttttga tctggtttgc
    32461 cgcggcagcg tggcggaagg ttgtaaactg cacctcgaag aacaagaggt cgaatgctgg
    32521 tgtgaaacat gccaacagta tgtgacgcta ttgacccagc gcgtccgccg ctgtccacag
    32581 tgtcatggtg acatgctaca gattgtggca gacgacggtt tacagattcg gcggatagaa
    32641 atagaccagg agtgagcgat gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc
    32701 gagggtgatg aacataaccc tcattccgcg tttcgtagcg cgccatttgc cccggcggca
    32761 cgcccgaaga tgaaaatcac cggcattaaa gcgcctgaat ttacccccag ccagactgaa
    32821 gaaggcgacc tgcattacgg tcatggcgaa gcgggcactc acgcaccggg catgagccag
    32881 cgtcggatgc tggaagtcga aattgacgtg ctggacaaaa acaaccgtct ggctgaacgc
    32941 aaccgcgcgc gctttgctgc ccgcaagcaa ctggtgctca acctggtttc cagcccaggt
    33001 tccggtaaaa ccaccctgct gacggaaacc ctaatgcgcc tgaaagacag cgttccgtgc
    33061 gcggttattg aaggtgacca gcaaaccgtg aacgatgccg cacgcattcg cgctaccggc
    33121 acaccagcga ttcaggtgaa cacaggtaaa ggctgccatc ttgacgcaca gatgattgcc
    33181 gacgccgcac cgcgtctgcc actggacgat aacggtattc tgtttatcga aaacgttggc
    33241 aacctcgtat gcccggccag cttcgatctc ggtgaaaaac acaaagtggc ggtgctttcc
    33301 gttaccgaag gtgaagacaa accgctgaaa tatccgcata tgtttgccgc cgcctcgctg
    33361 atgctgctca acaaagttga cctgttgccg tatctcaact ttgacgttga gaagtgcatc
    33421 gcctgcgccc gcgaagtcaa tccagaaatt gaaatcatcc ttatttccgc caccagcggt
    33481 gaagggatgg accagtggct gaactggctg gagacacagc gatgtgcata ggcgttcccg
    33541 gccagatccg caccattgac ggcaaccagg caaaagtcga cgtctgcggc attcagcgcg
    33601 atgtcgattt aacgttagtc ggcagctgcg atgaaaacgg tcagccgcgc gtgggccagt
    33661 gggtactggt acacgttggc tttgccatga gcgtaattac tgaagccgaa gcacgcgaca
    33721 ctctcgacgc cttacaaaac atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg
    33781 gcgaggaaaa ataatgcgtt ttgttgatga atatcgcacg ccggaacagg tgatgcagtt
    33841 aattgagcat ctgcgcgaac gtgcttcaca tctctcttac accgccgaac gccctctgcg
    33901 gattatggaa gtgtgcggtg gtcataccca cgctattttt aaattcggcc tcgaccagtt
    33961 actgcctgaa aacgttgagt ttatccacgg tccgggttgc ccggtgtgcg tactgccgat
    34021 gggtagaatc gacacctgcg tggagattgc cagccatccg gaagtcatct tctgtacctt
    34081 tggcgacgcc atgcgcgtac cagggaaaca gggatcgctg ttgcaggcaa aagcacgcgg
    34141 tgccgatgtg cgcatcgttt actcgccgat ggatgcgttg aaactggcgc aggagaatcc
    34201 aacccgcaaa gtggtgttct tcggcttagg ttttgaaacc accatgccga ccaccgccat
    34261 cactctgcaa caggcgaaag cgcgtgatgt gcagaatttt tacttcttct gtcagcacat
    34321 tacgcttatc ccgacgctgc gcagtttgct ggaacagccg gataacggta tcgacgcgtt
    34381 cctcgcgccg ggccacgtta gtatggttat cggcactgat gcctataatt ttatcgccag
    34441 cgattttcat cgtccgctgg tggtcgctgg tttcgaaccc cttgatctac tgcaaggcgt
    34501 ggtgatgctg gtggagcaga aaatagaggc ccacagcaag gtagagaatc agtatcgtcg
    34561 ggtggtaccg gatgccggta acctgctggc gcaacaggcg attgccgatg tgttctgtgt
    34621 caacggcgac agcgaatggc gcggcttagg cgtgattgaa tcttctggcg tgcacctgac
    34681 gccggattat caacgattcg atgccgaagc acatttccgc ccggcaccgc agcaggtctg
    34741 cgatgacccg cgcgcgcgtt gtggtgaggt attaacgggc aaatgtaagc cgcatcaatg
    34801 cccgctgttt ggtaacacct gtaatcctca aaccgcattt ggtgcactga tggtctcctc
    34861 cgaaggagcg tgcgccgcgt ggtatcagta tcgtcagcag gagagtgaag cgtgaataat
    34921 atccaactcg cccacggcag cggcggccag gcgatgcagc aattaatcaa cagcctgttt
    34981 atggaagcct ttgccaaccc gtggctggca gagcaggaag accaggcacg tcttgatctg
    35041 gcgcagctgg tagcagaagg cgatcgtctg gcgttctcca ccgacagtta cgtcattgac
    35101 ccgctgttct ttcctggcgg taatatcggc aagctggcga tttgcggcac cgcgaatgac
    35161 gttgcggtca gtggcgctat tccgtgctat ctctcctgtg gctttatcct cgaagaagga
    35221 ttgccgatgg agacactgaa agccgtagtg accagcatgg cagaaaccgc ccgcacggca
    35281 ggcattgcca tcgttactgg cgatactaaa gtggtgcagc gcggcgcggc agataaactg
    35341 tttatcaaca ccgctggcat gggcgcaatt ccggcgaata ttcactgggg cgcacagacg
    35401 ctaaccgcag gcgatgtatt gctggttagc ggtacactcg gcgaccacgg ggcgactatc
    35461 cttaacctgc gtgaacaact ggggctggat ggtgaactgg tcagcgactg cgcggtgctg
    35521 acgccgctta ttcagacgct gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc
    35581 cgtggtggtg taaacgcggt ggttcatgag ttcgcggcag cctgcggttg tggtattgaa
    35641 atttctgaat cagcactgcc ggttaaacct gctgtgcgcg gcgtttgcga actgctggga
    35701 ctggacgccc ttaactttgc caacgaaggc aaactagtaa tagctgttga acgcaacgca
    35761 gcagagcaag tgctggcagc gttacattcc catccactgg ggaaagacgc ggcgctaatt
    35821 ggtgaagtgg tggaacgtaa aggtgttcgt cttgccggtc tgtatggtgt gaaacgaacc
    35881 ctcgatttac cacacgccga accgcttccg cgtatatgct aataaaattc taaatctcct
    35941 atagttagtc aatgaccttt tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc
    36001 atatacaccg atgagtgatc tcggacagca agggttgttc gacatcactc ggacactatt
    36061 gcagcagccc gatctggcct cgctgtgtga ggctctttcg caactggtaa agcgttctgc
    36121 gctcgccgac aacgcggcta ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta
    36181 cgcatcgcgt gaaaaagaca cccccattaa atatgaagac gaaactgttc tggcacacgg
    36241 tccggtacgc cgcattttgt cgcgccctga tacgctgcat tgcagttacg aagaattttg
    36301 tgaaacctgg tcgcaggtgg gctatacccc aaatttggtc actattgcct gatgccactg
    36361 gcggcggaag ggcatatttt tggtggctgt gaatttattc gttatgacga tcgtccctgg
    36421 agcgaaaaag agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa
    36481 caaatccaga gccgcgtcgt caacaatgtc gactatgagt tgttatgccg ggagcgcgat
    36541 aacttccgca tcctggttac cattaccaac gcggtgcttt cccacctgga tatggacgaa
    36601 ctggtcagcg aagtcgccaa agaaatccat tactatttcg acattgacga tatcagtatc
    36661 gtcttacgca gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa
    36721 caacatcccg cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg
    36781 ttcaaaagta aagagatgct gctgatcaat ctccacgagc gggacgattt agccccctat
    36841 gaacgcatgt tgttcgacac ctggggcaac cagattcaaa ccttgtgcct gttaccgctg
    36901 atgtctggcg acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt
    36961 accactacca atctgaattt gctgcgccag attgccgaac gtgtggcaat cgctgtcgat
    37021 aacgccctca cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc
    37081 gtcctgaccg agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc
    37141 gaagccatgt acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg
    37201 ctgatcctcg gtgaaactgg cacgggtaaa gagctgattg cccgcgcgat ccataatctc
    37261 agtgggcgta ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggattg
    37321 ctggaaagcg atctgtttgg tcatgagcgt ggggctttta ccggtgccag cgcccagcgc
    37381 attggtcgtt ttgaactggc ggataaaagc tccctgttcc tagacgaagt gggcgatatg
    37441 ccactggagt tacagccgaa gttgctgcgt gtattgcagg aacaggagtt tgaacgtctc
    37501 ggcagcaaca aaatcattca gacggacgta cgtttaattg ccgcgactaa ccgcgatctg
    37561 aaaaaaatgg tcgccgaccg agagttccgt agcgatctct attaccgcct gaacgtattc
    37621 ccgattcacc tgccgccact acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc
    37681 tttaccttca aaattgcccg tcgtctgggg cgcaatatcg acagcattcc agccgagacg
    37741 ctgcgcacct tgagcaacat ggagtggccg ggtaaagtac gcgaactgga aaacgtcatt
    37801 gagcgcgcag tattgctaac acgcggcaac gtgctgcagc tgtcattgcc agatattgct
    37861 ttaccggagc ctgaaacgcc gcctgccgca acggttgtcg ctcaggaggg cgaagatgaa
    37921 tatcagttga ttgttcgcgt gctgaaagaa accaacggcg tggttgccgg gcctaaaggc
    37981 gctgcgcaac gtctggggct gaaacgcacg accctgctgt cacggatgaa gcggctggga
    38041 attgataaat cggcattgat ttaactgcaa attgccggac agatctgcct gtccggcata
    38101 ctattcacga gcttttttcg gacgatattt ttccggcagt tctggcaccg gacgcttgtc
    38161 atcgatgaga tgacgcacgg ttaagatcgg atgacgccac agcattctcg gcccggccca
    38221 acgcataatc tgtttcatct cttcacgctt tgcaggctgg taacagtgca ccggacactg
    38281 cttacaggct ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaaac
    38341 aaacaacgcc tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag
    38401 atcgatcatt tttttaatcg tcagtttttc acgagagata cgcttgccgg acatgctgcc
    38461 tccatactct taaggtgtat ttatattaca acttaatttg aaaggggact atggctctga
    38521 aggaggaggt ttattcaacc gacgcaattt tgcgtacttg cttcataagc atcacgcaaa
    38581 agctgcaaaa cagcatcttt cccggaacca gcatcaagaa ctcgccgttc gcttcttccc
    38641 ctgaaatgat taactccggt atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc
    38701 atcacacccc atttaatatc aggaaccgga cataacccca tgagtgcaat agaaaatttc
    38761 gacgcccata cgcccatgat gcagcagtat ctcaggctga aagcccagca tcccgagatc
    38821 ctgctgtttt accggatggg tgatttttat gaactgtttt atgacgacgc aaaacgcgcg
    38881 tcgcaactgc tggatatttc actgaccaaa cgcggtgctt cggcgggaga gccgatcccg
    38941 atggcgggga ttccctacca tgcggtggaa aactatctcg ccaaactggt gaatcaggga
    39001 gagtccgttg ccatctgcga acaaattggc gatccggcga ccagcaaagg tccggttgag
    39061 cgcaaagttg tgcgtatcgt tacgccaggc accatcagcg atgaagccct gttacaggag
    39121 cgtcaggaca acctgctggc ggctatctgg caggacagca aaggtttcgg ctacgcgacg
    39181 ctggatatca gctccgggcg ttttcgcctg agcgaaccgg ccgaccgcga aacgatggcg
    39241 gcagagctgc aacgcactaa tccggcggaa ctgctgtatg cagaagattt cgccgagatg
    39301 tcgctgattg aaggccgtcg cggcctgcgc cgtcgcccgc tgtgggagtt tgaaatcgac
    39361 accgcgcgcc agcagttgaa tctgcaattt ggcacccgcg atctggtcgg ttttggcgtc
    39421 gagaacgcgc cgcgcggact ttgtgctgcc ggttgtctgt tgcagtatgc gaaagatacc
    39481 caacgcacga ccctgccgca tattcgttct atcaccatgg aacgtgagca ggacagcatc
    39541 attatggatg ccgcgacacg tcgtaacctg gaaattactc agaacctggc gggcggtgcg
    39601 gaaaatacgc tggcttctgt gctcgactgc accgtcacgc cgatgggtag tcgtatgctg
    39661 aaacgctggc tgcatatgcc agtgcgcgat acccgcgtgt tgcttgagcg ccagcaaact
    39721 attggcgcat tgcaggattt caccgccggg ctacagccgg tactgcgtca ggtcggcgac
    39781 cttgaacgta ttctggcacg tctggcttta cgaactgctc gcccacgcga tctggcccgt
    39841 atgcgccacg ctttccagca actgccggag ctgcgtgcac agttagaaac tgtcgatagt
    39901 gcaccggtac aggcgctacg tgagaagatg ggcgagtttg ccgagctgcg cgatctgctg
    39961 gagcgagcaa tcatcgacac accgccggtg ctggtacgcg acggtggtgt tatcgcatcg
    40021 ggctataacg aagagctgga tgagtggcgc gcgctggctg acggcgcgac cgattatctg
    40081 gagcgtctgg aagtccgcga gcgtgaacgt accggcctgg acacgctgaa agttggcttt
    40141 aatgcggtgc acggctacta cattcaaatc agccgtgggc aaagccatct ggcacccatc
    40201 aactacatgc gtcgccagac gctgaaaaac gccgagcgct acatcattcc agagctaaaa
    40261 gagtacgaag ataaagttct cacctcaaaa ggcaaagcac tggcactgga aaaacagctt
    40321 tatgaagagc tgttcgacct gctgttgccg catctggaag cgttgcaaca gagcgcgagc
    40381 gcgctggcgg aactcgacgt gctggttaac ctggcggaac gggcctatac cctaaactac
    40441 acctgcccga ccttcattga taaaccgggc attcgcatta ccgaaggtcg ccatccggta
    40501 gttgaacaag tactgaatga gccatttatc gctaacccgc tgaatctgtc gccgcagcgc
    40561 cgtatgttga ttattaccgg cccgaacatg ggcggtaaaa gtacctatat gcgccagacc
    40621 gcactgattg cgctgatggc ctacatcggc agctatgtac cggcacaaaa agtcgagatt
    40681 ggacctatcg atcgcatctt tacccgcgta ggcgcggcag atgacctggc ttccgggcgc
    40741 tcaaccttta tggtggagat gactgaaacc gccaatattt tacataacgc caccgaatac
    40801 agtctggtgt taatggatga gatcgggcgt ggaacgtcca cctacgatgg tctgtcgctg
    40861 gcgtgggcgt gcgcagaaaa tctggcgaat aagattaagg cattgacgct gtttgccacc
    40921 cactatttcg agctgaccca gctaccggag aaaatggaag acgtcgccaa cgtgcatctc
    40981 gatgcgctgg agcacggaga caccattgcc tttatgcata gcgtgcagga tggcgcggcg
    41041 agcaaaagct acggcctggc ggttgcagct ctggcaggcg tgccaaaaga ggtgattaag
    41101 cgcgcacggc aaaaactgcg tgagctggaa agcatttcgc cgaacgccgc cgctacgcaa
    41161 gtggatggta cgcaaatgtc tttgctgtca gtaccggaag aaacctcgcc agcggtcgaa
    41221 gctctggaaa acctggaccc ggattcactg actccgcgtc aggcgctgga atggatttat
    41281 cgcttgaaga gcctggtgta ataacaattc ccgatagtct tttgctatcg ggaatattaa
    41341 cgacaactga cgaataaaat aaaaacaccc tgtataatag gaaagcttat tttacagggt
    41401 aaaaccatgc catctacacg ctatcaaaaa attaatgccc atcactatcg ccatatatgg
    41461 gtcgttggtg atattcatgg tgaatatcaa ttattacaat cccgcttaca tcaactctct
    41521 ttttgccctg aaaccgactt acttatttct gtcggcgata atattgatcg tgggccggag
    41581 agtcttgacg tcctgcgcct gctaaaccag ccctggttta tctccgttaa aggcaaccac
    41641 gaagcaatgg cgctggatgc gttcgcgacc ggcgatggca atatgtggct tgccagcggt
    41701 ggtgactggt ttttcgattt aaatgattca gagcaacaag aggcaataga tctgttgctg
    41761 aaattccatc accttccaca tattattgaa atcactaacg acataataaa atatgtcatc
    41821 gcacatgcag attatccggg tgatgaatat ctctttggca aagaaatagc agagagcgaa
    41881 ttactctggc ctgtttatcg tgtgcagaaa tcgcttaatg gcgagttaca ataaataaac
    41941 ggcgctgatt attttatatt tggacatatg atgtttgata acattcagac gttcgctaac
    42001 cagatttata ttgataccgg atcgccgaaa agtgggcggc tgtcatttta taaaataagg
    42061 tagtctcatg cttcttctgt gaagcatgag taaccagatg ttattgcagg ccattattca
    42121 tttttcgcta ccagcaaaga gagatcctgc ttcgccagcg cgcgactggc actctccggc
    42181 aaaccgtcgt ctgtaataat ctgatcaaac tcgcttaatg gtaacgccag ccatgtcgcc
    42241 acctgaccat atttcgtcgc atcacagacc aaaactcgct ggcggctggc actagcaatc
    42301 gcccgtttca ccgtgacttt atcttccgct ggcgtagaaa tcccccgcac actccatgac
    42361 gatgcagaaa taaaagcctg atcaatcatc aggctgcgca gcatggtcgc agcggcttcc
    42421 ccgacacagg aacggttttc ccgacacact gcaccgccag tgtgaataat tgtgcaatta
    42481 ctgttgtcga gcaagtagtt cgcaataacg aaatcgtttg tgaccacagt cagtgactcc
    42541 atgtgaatca gatgctgtgc tatcgctaac gtggttgttc ccgcatccag atagatacaa
    42601 cttcccggct gaacaagact tgccgccagc ttgccaatag ccgctttttg cgtcattgcc
    42661 agcgcagttt ttacctgatg agaaggttca tgcgccacgc gtcccggaga ctggacgcct
    42721 ccggacacca gcacaacggc tccctgctgc tccagttttt gtaaatcccg acgaatggtc
    42781 atatgtgaca cattcattct gtccgttagt tcagcaatac tgacaatgcc tttttcagct
    42841 accatctcaa ggatgatttg gcgacgctct acgggtatca acttttgctc cttcctttgt
    42901 cctgctgaca ttctacgcta tttgcctgcg aaacgtgcgc ggcgcaacta acgcttagtt
    42961 cacataaaat aacacacaat gttaatttat gtgaatcagc tcaccatatc gttatcttcc
    43021 agcgcttata ttcacaatat caaacaaaat atcacttaaa ttaacaagga gagcagatga
    43081 aaacgggatc tgagtttcat gtcggtatcg ttagcttagg gtcaatggga atgggagcag
    43141 cactgtcatg tgtccgcgca ggtctttcta cctggggcgc agacctgaac agcaatgcct
    43201 gcgctacgtt gaaagaggca ggtgcttgcg gggtttctga taacgccgcg acgtttgccg
    43261 aaaaactgga tgctctgctg gtgctggtgg tcaatgcggc ccaggttaaa caggtgctgt
    43321 ttggtgaaac aggcgttgta caacatctga aacccggtac ggcagtaatg gtttcttcca
    43381 ctatcgctag tgctgatgcg caagaaattg ctaccgctct ggctggattc gatctggaaa
    43441 tgctggatgc gccagtttct ggtggtgcag taaaagccgc taacggtgaa atgactgtca
    43501 tggcctccgg tagcgatatt gcctttgaac gactggcacc cgtgctggaa gccgttgccg
    43561 gaaaagttta tcgcataggt gcagaaccgg gactaggttc gaccgtaaaa attattcacc
    43621 agttgttagc gggcgtacat attgctgccg gagccgaagc gatggcactt gcagcccgtg
    43681 cggggatccc gctggatgtg atgtatgacg tcgtgaccaa tgccgccgga aattcctgga
    43741 tgttcgaaaa ccggatgcgt catgtggtgg atggcgatta caccccgcat tcagccgtcg
    43801 atatttttgt taaggatctt ggtctggttg ccgatacagc caaagccctg cacttcccgc
    43861 tgccattggc ctcaacagca ttgaatatgt tcaccagcgc cagtaacgca ggttacggga
    43921 aagaagacga tagcgcagtt atcaagattt tctctggcat cactctaccg ggagcgaaat
    43981 catgatcaag attggcgtta tcgccgatga ttttaccggc gcgacggata tcgccagttt
    44041 tctggtggaa aacggtctac caacggtaca aattaacggt gttccaacag gtaaaatgcc
    44101 ggaagcaatc gacgcactgg tgatcagcct gaaaacgcgc tcctgtccgg tggttgaagc
    44161 cacacagcaa tcgctggcgg ctctgagctg gttgcaacag caaggttgca aacagatcta
    44221 tttcaaatac tgctctactt tcgacagtac ggcgaaaggt aatatcggcc cggttaccga
    44281 tgcattaatg gatgctctcg acacgccgtt tacggtcttc tctccggccc tgtcggtcaa
    44341 cggacgtacg gtttatcagg ggtatttgtt cgtaatgaat caactgctgg ccgaatccgg
    44401 gatgcgccat cacccggtaa atcccatgac cgacagctat cttccccgtc tggttgaagc
    44461 acaatccaca gggcgctgcg gcgtcgtttc ggcacatgtt ttcgaacaag gtgtggatgc
    44521 cgttcgtcaa gagctggctc gcttacagca agagggctac cgctacgcgg tgcttgatgc
    44581 gctgaccgaa caccatctgg aaattcaggg agaagccttg cgcgatgccc cactggtaac
    44641 gggcggttct ggtctggcga ttggcctggc ccggcagtgg gcgcaagaaa acggtaacca
    44701 ggctcgcgaa gcagggcatc cgctcgctgg gcgcggcgta gtactctccg gttcatgctc
    44761 tcaaatgacc aaccgccagg tggcacatta ccgtcaaatt gcaccagccc gtgaagttga
    44821 tgtggcacgc tgcctctcaa ccgaaactct ggccgcttat gcacacgaac tggcagagtg
    44881 ggttctgggc caggaaagtg tacttgctcc actggttttt gccaccgcca gcactgacgc
    44941 attggcagca attcaacagc aatacggtgc acaaaaagcc agtcaggcag tagaaactct
    45001 gttttctcaa ctagcggcgc ggttagcagc ggaaggcgtg acacgcttta ttgtcgcagg
    45061 cggtgagacc tccggcgtag tcacacagag cctgggcata aaagggtttc atattggccc
    45121 aaccatttcc cccggcgtgc cgtgggtaaa cgcactggat aagcctgtct cactcgccct
    45181 taaatctggc aacttcggtg atgaagcctt tttttcacga gcccaaagag agtttttatc
    45241 atgagcgatt tcgcaaaagt agagcagtct ttgcgagagg agatgacgcg gattgccagt
    45301 tcattctttc agcgcggcta tgcaaccggt tcggctggca atctgtcgct gcttttacct
    45361 gacgggaatt tactggcgac accgacaggt tcatgcctgg gcaatctcga tccgcagcgg
    45421 ctttccaaag tcgccgcgga tggcgaatgg ttaagtggtg acaaaccctc gaaagaggtg
    45481 ctctttcatc tggcgctgta tcgcaacaat ccgcgctgta aagcggtggt gcatttgcac
    45541 agcacatggt cgacggcgct ttcctgcctg caagggctgg acagcagcaa cgttattcgt
    45601 ccgttcacac catacgtggt gatgcggatg ggaaatgtcc cgctggtgcc ctattaccga
    45661 ccgggcgata aacgcatcgc acaggatctg gcggaactgg cagcagacaa tcaggctttt
    45721 ttactggcaa atcatggccc agtggtttgc ggtgaaagcc tgcaagaagc caccaacaat
    45781 atggaagagc tggaggaaac ggcaaagctg atttttattc tcggtgaccg cccgatccgt
    45841 tatctgaccg caggtgaaat tgcggaatta aggagttaat gcaatgcctc gttttgcagc
    45901 taatttatcc atgatgttca ccgaagtgcc ttttattgaa cgcttcgccg cagcgcgaaa
    45961 agccgggttc gatgctgtgg aatttctgtt tccctatgac tactccaccc tgcaaatcca
    46021 aaagcaactg gagcaaaacc atctgacact ggcgctgttc aataccgctc ccggagatat
    46081 taatgcgggg gaatggggat tatccgccct tcccggacgt gagcacgaag cacacgcaga
    46141 catcgacctg gcactggaat atgcattggc gctcaattgt gaacaagtcc atgtgatggc
    46201 aggcgtcgtg cccgctggcg aagatgctga acggtaccgg gcagtattta tcgataatct
    46261 ccgctacgct gccgaccgct ttgcgccaca cggcaagcga atattagttg aagcactcag
    46321 ccccgacgtt aagcctcatt atctcttctc cagccagtat caggcactgg ctattgttga
    46381 agaggttgcg cgagataacg tgtttattca actagatact tttcatgcgc aaaaagtaga
    46441 tggcaacctg acgcatttaa tccgcgacta cgctgggaaa tatgcgcacg tacaaattgc
    46501 cggactacct gatcgacatg aaccggacga tggagaaatc aactatccgt ggctgttccg
    46561 cctgttcgat gaggtgggat atcagggctg gatcggttgt gaatataaac ctcgtggcct
    46621 caccgaagaa gggcttggct ggtttgacgc ctggcgctaa ttcgttaaat ccctaattac
    46681 aacgtaccca tacatccccc catctacgga tggggtaggt taactataac tttcagacag
    46741 ggtttcccat gtccacaatt acattgttat gcattgcgtt agctggcgta atcatgctgt
    46801 tgctgctggt catcaaggca aaggtacaac cattcgttgc tctgctcctc gtcagcctgt
    46861 tagtcgcact tgcggcgggt ataccggcgg gcgaagtggg taaagtgatg atcgccggga
    46921 tgggcggtgt gcttggttcc gtcactatta ttattggtct gggcgctatg ctggggcgta
    46981 tgatcgaaca ctctggtggt gcagagtcac tggctaatta tttcagtcgc aagttaggtg
    47041 acaaacgaac tatcgctgcg ctgactctgg cagtgttctt cctcggtatt cccgtcttct
    47101 ttgatgtcag ctttattatt cttgcgccaa tcatttacgg ttttgccaag gttgccaaaa
    47161 tatcgccact caaatttggc ctgcctgtcg ctggaatcat gctaactgtt catgtggcag
    47221 taccgccgca tccaggccct gtcgccacag cggggttact ccacgcagac atcggctggc
    47281 taaccatcat cggtattgcg atttctattc ccgtagggat tgttggctac tttgcagcga
    47341 aaataatcaa taagcgccaa tatgcgatgt cagtagaagt actggaacag atgcaactgg
    47401 ctccggccag tgaggaaggc gcgacaaaat taagcgataa aataaatcca ccgggcgtcg
    47461 cgctggtcac ctcgctaatt gttattccta tcgcgattat catgacgggt acggtttccg
    47521 caacactgat gccgccttcg catcccctgc ttggtacgct acagctgatc ggctcaccaa
    47581 tggtagctct aatgattgcg ctggtgctgg cattctggtt attggcttta cgtcgcggct
    47641 ggagcttaca acacaccagc gacattatgg gctcagccct tcccactgcg gcggtagtga
    47701 ttttggttac tggtgctgga ggggtatttg gcaaagtgct ggtggaatcg ggcgtcggca
    47761 aagcccttgc caatatgcta caaatgattg acctgcctct gttaccagcc gcatttatta
    47821 tttcactggc tctgcgtgca tcgcaggggt cagcaaccgt agcaatcctg acaaccggcg
    47881 ggttactctc agaagcggtg atgggattga atccgattca gtgcgtattg gtgacgctgg
    47941 cagcctgctt tggtgggctt ggcgcgtcac atattaatga ctcagggttc tggattgtga
    48001 ccaaatatct ggggttgtcg gtagcagacg gtctgaaaac ctggacagtg ttaacgacca
    48061 ttctcggttt taccggattc ttaattacct ggtgcgtatg ggcggcaatt tgaccttaat
    48121 aaaaaggtcc gatgggcatc ggacctttta ttgtgcacag aaaaggccag cctcgcttga
    48181 gactggcctt tctgacagat gcttacttac tcgcggaaca gcgcttcgat attcagcccc
    48241 tgcgtttgca ggatttcacg caaacggcgc aggccttcaa cctgaatctg gcgaacacgt
    48301 tcacgggtga ggccaatttc acgacctaca tcttccagtg ttgccgcttc gtaccccagc
    48361 aaaccgaatc gacgtgccag cacttcacgc tgtttggcgt tcagctcgaa cagccatttg
    48421 acgatgctct gcttcatatc gtcatcttgc gtggtatctt ccggaccgtt ctctttttca
    48481 tcggccagga tgtccagcaa cgctttttcg gaatcaccac ccagcggggt gtctaccgag
    48541 gtaatgcgct cgttaagacg aagcatacgg ctgacgtcat caactggctt atccagttgc
    48601 tctgcgatct cttccgcact tggttcatgg tccagcttat gggacaactc acgtgcggtt
    48661 cgcaggtaaa cgttcagctc ctttacgatg tgaatcggca aacgaatagt acgggtttgg
    48721 ttcataatcg cccgttaaat cgtctggcga atccaccagg ttgcgtatgt tgagaagcgg
    48781 aaaccacgtt ccgggtcaaa cttctctacc gcgcggatca gccccaggtt gccctcttcg
    48841 ataaggtcca gcaacgccag accacgattg ccataacggc gggcaatttt taccaccaga
    48901 cgcaagttac tctcgatcat ccggcggcga gaggcgacat ctccacgcag tgcgcgacgc
    48961 gcaaaataaa cttcttcttc ggccgttaac agtggtgaat aaccaatctc accaaggtaa
    49021 agctgagtcg cgtccaacac acgctgtgtg gctccctgcg ataacagttc ctcttcggcc
    49081 aaatcgttat cactgggttc ctcttctact aaggcctttt cgtcaaaaac ctcaactccg
    49141 ttctcatcaa attccgcatc ttcatttaaa tcatgaactt tcagcgtatt ctgactcata
    49201 aggtggctcc tacccgtgat cccttgacgg aacattcaag caaaagcctg gttccgccga
    49261 tttatcgctg cggcaaataa cgcagcgggt ttacggattt ccccttgtaa cgaatttcaa
    49321 aatgcaagcg tgttgaactg gttccggtgc tacccatggt tgctattttt tgccccgcct
    49381 tcacttcttg ttgttcccgg accagcattg tgtcgttatg ggcgtaggca ctcaggtaat
    49441 catcattatg tttgatgata atcagattac cgtagccgcg cagcgcgtta ccggcataaa
    49501 caacgcggcc atctgcggtc gcgataattg cctgtccttt gctgcctgcg atatcaatcc
    49561 ccttgttgcc cccctcagaa gcgccaaagg tttcgatcac tttgccctca gtcggccagc
    49621 gccaggtgga gataggcgta ctggttgatg tactgctgac agttggctcg gttgtgcttg
    49681 ctgttggtac cgttacaggc gctgtgaccg tggtcgcagc tggcttgttg ttcggcaaca
    49741 ttttgttagc actctgttca cccgaagatt cagaatacgt aattgtcggt tgcgacgcaa
    49801 cagcaacggt ggaattttgt gcaggcttga tcacaactcc ttgctctgct gcgtcggcct
    49861 gggtaatggc atttccgcca gtgattggcg taccggaagc attacccacc tgcaaggtct
    49921 gaccaacatt cagcgcgtat ggtgcctgaa tattgttgcg ctgagcaagg tcacggaaat
    49981 cgttgccagt aatccaggcg atatagaaaa gtgtgtcgcc ttttttcacg gtataggtac
    50041 tgccgctata actgcctttc ggaatgttcc catactgacg gttatagacg atgcgtccgt
    50101 tttccatctg taccggctgc tgagctactg gctgcactgg ctggatttgc ggttgctggg
    50161 ctgtagacgt cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttggtatttg
    50221 caggcgcatt gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc
    50281 ctgccagcca tagcgaaacc agtgacaaag ccgcaatgcg gcgaacggtg aattttgggc
    50341 ttcccgcgct catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt
    50401 gcaaggcacc ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat
    50461 aaccaggaaa aatccaggta tttcctcacg ttttaagcca gctccccctt cactaaaggg
    50521 acaaagcgca cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt
    50581 ttcaaatact ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc
    50641 gtcattagcg cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc
    50701 gcgcgtgcct gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca
    50761 agatttttca ggcggcgacg cgcctgccac tgcaaacctt taatccgttc gaccgagcaa
    50821 acatgctgga caagatgcgc caggattgcc gtttgatatc ccgaaccggt gccaatttcc
    50881 agcacccgcg actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc
    50941 tgcgaaattg tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca
    51001 aacgcttcat caacgaattt ttcacgcggc acggcagcaa gtgcattcag cacctgctca
    51061 tcctgaatac cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt
    51121 gcgtgccaac tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag
    51181 ttaaatccac atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg
    51241 tccccggacc ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc
    51301 gcggatcttg ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc
    51361 gaataccttt gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg
    51421 tgcgcagcgg ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt
    51481 cgtaatgttt atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt
    51541 ccatcgcggc ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt
    51601 taatgccgga cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga
    51661 cgcaatcggt cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc
    51721 gcagggagga ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga
    51781 ccacctgaac gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat
    51841 gtaccccgtc atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc
    51901 ctgacaacgc tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg
    51961 tcatcccacc aattccagct taattgttgc ggatacagca gcatcgctct gcgcgcggct
    52021 tcaacttttt cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgcttgt
    52081 tcgaatgcca gcgcttcacg ctgagttccc cattcgccac tgcccggcaa tgcggcggtt
    52141 atcatcagct ctttatcgtt gacgcgatgc tgtaattccg ccagttcttc ggtggttgct
    52201 acaaaccagc taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg
    52261 tctgcttttt tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac
    52321 aaccaaaaac tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgcagc
    52381 gcgccctgca agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca
    52441 ccttttacgc aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc
    52501 agaaccaggg taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc
    52561 gcatactcca gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg
    52621 cctggcacgc gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg
    52681 aagctgactt cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg
    52741 gtattgcagc cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca
    52801 aagcccaaat cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct
    52861 tgcggtttac cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga
    52921 gtagcgccac cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg
    52981 tagtagtggc tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca
    53041 cgcgcatttg tggaatgtgc ggcaacatct tcggtgcctg agcgatgata gtgacatcga
    53101 cgttgcccag ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc
    53161 ggctgtcggc acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca
    53221 gcgccgccgc gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat
    53281 gcgccagcaa tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac
    53341 cgccaaaggc atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat
    53401 ggttcgggtg aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt
    53461 atccgcacgg ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc
    53521 gtcggtaata gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg
    53581 agggaaaaat tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat
    53641 ggcatttttg cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc
    53701 tcccgtgcgg ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca
    53761 aggacgagcg gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc
    53821 cagcacggaa tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc
    53881 cagaggaagt tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac
    53941 ccggggatgc gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga
    54001 gagatattgc ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac
    54061 ggcgcaaaca tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt
    54121 ttgccccgca gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc
    54181 cggcctggtc atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt
    54241 gagatcgtca atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc
    54301 ttgctgtgcc gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa
    54361 ccacagcgaa tactgtagcc agatcagaat agccagcaac agcagcgtta gtttacccat
    54421 cctgccccct gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct
    54481 gttggggata ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc
    54541 cagggcgtgc agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac
    54601 aagaaagtac aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg
    54661 tcaccagggt gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa
    54721 tccccaccac taccgcgaca ggcatcagtg ccagaaagaa aggccaggtg tagataaaga
    54781 agaacagcgt gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga
    54841 taaaaccgac cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt
    54901 cgttatttgt tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga
    54961 accgggcatg gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga
    55021 tctaataatt gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc
    55081 gattcaggcg cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc
    55141 gctttcttat ataagccttt gggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca
    55201 aacacttcga taaagcgccc ttctcctacg cgttcgcgaa ccatctggcg ttcggcgcgg
    55261 tgtggcgaga taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact
    55321 tcaccgacgc gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat
    55381 aatccgtggc gaacattgtc gccatccagc agatacgtac tgacgccgag tttatgtaac
    55441 gcctcctcca gcaccccggc gaccgttgat ttaccggacc cagagaggcc ggtaaaccac
    55501 agcactacac cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta
    55561 tgccagacga cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc
    55621 cccagtgcgg aaagtggcga cgaaccaggg cattcaattc cagttcgaat gcactgaatt
    55681 cagatgacgc aacggcggcc tcgtttttcg gctcatgcac cataccggca ccaacagtca
    55741 cgttgctcag gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat
    55801 ctaacaccag cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt
    55861 tttcaacttc acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac
    55921 gcgcgcgcgt cttcttaccg gcaattttga tgtcgtaact ctggcccggg gaaagcggct
    55981 gttccgccat ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg
    56041 ccgccagcag cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg
    56101 cttctccggc aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg
    56161 attccacacc agagggcagc gctttgacac gttgcccgac ttccacgcga ccggatgcca
    56221 gcgttccggc gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc
    56281 gcattggctg ggcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg
    56341 tcggaccgct gtaccacgac atactttcac tttgcgatgc cacgttgtcg ccttccagtg
    56401 cagagagcgg cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcaaat
    56461 aatcttcacg aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga
    56521 tcgccacgac cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt
    56581 gatcgagcac gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc
    56641 cagtcgccat attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac
    56701 gcttctcggt agagaaataa cggtaagcca cgtcaatggt gatgccctgt tcgcgctcag
    56761 cttgcaggcc gtccaccagc agtgccagat ccagcttttc gccctgggtg ccgtgacgct
    56821 tactgtcgtt atgcagcgat gagagctgat cttcgtagat ttggcgggta tcgtgcagca
    56881 ggcgaccaat cagggtactt ttgccgtcat cgacgctacc acaggtcaga aaacgcagca
    56941 ggcttttatg ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt
    57001 gtgcaagtgc ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagacccc
    57061 gcctggtcgc ggtcaatcac gcggccctga cgttcactgg tggtagaaac cagcatctct
    57121 tcgatgatct ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag
    57181 cccagcgtac ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata
    57241 cggttgtcat caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg
    57301 agatacagtg gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg
    57361 gtccagttag agagcgggaa gacgcggatg ctttcacctt tgttaatttg cccgttgtag
    57421 ttgtgccaca gctccgggcg ctgatttttc ggagcccagc gatggaagcg gtcacggaaa
    57481 gagtagattc gctctttagc gcgggatttc tcttcatcac ggcgcgcacc accgaaggcg
    57541 gcatcaaaac cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta
    57601 tgtttcgcgc tgccgtgtac gaatggatta atccccatcg ccacgccttc cgggttttta
    57661 tgcaccagca gctcgcagcc gtaggcttta gcagtacgat cgcggaactc atacatctcg
    57721 cggaatttcc agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac
    57781 gccttgcgcg ccagatgcag catgacgctg gaatctttac cgatagagta gagcatcacc
    57841 ggatttgaga attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc
    57901 cgcaggtgag taagtcgttt ttgagccata accgttcctt tgcaataccg ctattttctt
    57961 gccatcagat gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat
    58021 gagtagttcc ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca
    58081 ccggatttcg ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact
    58141 atggcggtaa aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca
    58201 ccgtaccgct gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc
    58261 taaacctggc gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg
    58321 catgaccgga actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca
    58381 aatgggttat cgcagtgata ttcggacatt taatagtcgg tatatttata ccgcccgcga
    58441 taatcgtaag agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa
    58501 agcgccgcag cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc
    58561 tgacgccgat gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg
    58621 tttaggtgtc atgctggaat tggcagaacg cctgaaaaat acgcctaccg agtatggtat
    58681 tcgatttgtg gcgaccagcg gcgaagagga agggaaatta ggcgctgaga atttactcaa
    58741 gcggatgagt gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat
    58801 tgttggcgat aaattgtatt tcaacagcgg tgtaaaaacc actgaagtag taaggaaatt
    58861 aacgcgcgac agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc
    58921 ggggttgaat aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga
    58981 caaagcgggc attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga
    59041 tggttatcag caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt
    59101 aagactggat aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg
    59161 ccgtgacgtt atgcggataa tgctaccgtt ggtgaaggag ctggcgaagg cgtcttgatg
    59221 ggtttgaaaa tgggagctgg gagttctacc gcaggggcgg ggaattctaa gtgatatcca
    59281 ttatcgcatc cagtgcgccc ggtttatccc cgctggcgcg gggaacacac taagcataca
    59341 taactgtttt taaacaaatt tattccgcat caacaatcta ccaactaaat taaaacattt
    59401 ccttattttt aaagaacgga taacttattg attatcaaca ggaagaaaag aaaccaaacg
    59461 taacccatcc aaatccaccg gaatacgtct gttttctccc caggtctgaa attcaaaacc
    59521 cgactcggta ttggtcgccc aggccatcac cacatttccg caaccagcca gttgggtaat
    59581 ttgctgccag atcatctccc gaatacgttt tgatgtatca ccaacataca caccggcacg
    59641 cacttccagt agccagattg cgagccgtcc acgtaagcgc ggcgggacat tttctgtaac
    59701 aaccacgacc atgctcatca gccgcgtcct cgatgaccgc tatcgcccaa cgtttcaggt
    59761 tcggggatgg ctggcggtaa catatccggc gcaggttgtg gcggttcaat ttcacctgcg
    59821 gcaaggactt cctcaattaa cggtattaat ttgcccgtta acttagtgct acggaaaata
    59881 tcacgacagg ctaatctgac ttctttatca ggttctgcgg gttgcctcgc cgctatttca
    59941 aatgcctttg gtacaaccga atcaaattta atgatatcgg ctatgtcata aacaaatgaa
    60001 agcggtttgc cactatggat aaacccaata gcgggcgcat atcccgcggc t
//
LOCUS       DAJTNA010000003        54900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTNA010000003 DAJTNA010000000
VERSION     DAJTNA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54900
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.003"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..272)
                     /locus_tag="OO161_000126"
     CDS             complement(<1..272)
                     /locus_tag="OO161_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5461335.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="OO161_000127"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="OO161_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="OO161_000128"
     CDS             complement(1132..1338)
                     /locus_tag="OO161_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5461336.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="OO161_000129"
     CDS             complement(1445..2008)
                     /locus_tag="OO161_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5461337.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="OO161_000130"
     CDS             complement(3383..5218)
                     /locus_tag="OO161_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461338.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="OO161_000131"
     CDS             5319..5606
                     /locus_tag="OO161_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5461339.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="OO161_000132"
     CDS             5578..7107
                     /locus_tag="OO161_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5461340.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="OO161_000133"
     CDS             complement(7277..8494)
                     /locus_tag="OO161_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5461341.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(9012..11288)
                     /gene="clpA"
                     /locus_tag="OO161_000134"
     CDS             complement(9012..11288)
                     /gene="clpA"
                     /locus_tag="OO161_000134"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5461342.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(11319..11639)
                     /gene="clpS"
                     /locus_tag="OO161_000135"
     CDS             complement(11319..11639)
                     /gene="clpS"
                     /locus_tag="OO161_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5461343.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            11962..12186
                     /gene="cspD"
                     /locus_tag="OO161_000136"
     CDS             11962..12186
                     /gene="cspD"
                     /locus_tag="OO161_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5461344.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            complement(12259..14205)
                     /gene="macB"
                     /locus_tag="OO161_000137"
     CDS             complement(12259..14205)
                     /gene="macB"
                     /locus_tag="OO161_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5461345.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKSITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14202..15317)
                     /gene="macA"
                     /locus_tag="OO161_000138"
     CDS             complement(14202..15317)
                     /gene="macA"
                     /locus_tag="OO161_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5461346.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQELDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            15468..16424
                     /locus_tag="OO161_000139"
     CDS             15468..16424
                     /locus_tag="OO161_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5461347.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            complement(16421..18079)
                     /locus_tag="OO161_000140"
     CDS             complement(16421..18079)
                     /locus_tag="OO161_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5461348.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNIPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            18505..19200
                     /gene="aqpZ"
                     /locus_tag="OO161_000141"
     CDS             18505..19200
                     /gene="aqpZ"
                     /locus_tag="OO161_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5461349.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVSHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            19695..20594
                     /gene="lysO"
                     /locus_tag="OO161_000142"
     CDS             19695..20594
                     /gene="lysO"
                     /locus_tag="OO161_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5461350.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQASLKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            20738..22390
                     /gene="hcp"
                     /locus_tag="OO161_000143"
                     /gene_synonym="priS"
     CDS             20738..22390
                     /gene="hcp"
                     /locus_tag="OO161_000143"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5461351.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            22402..23370
                     /gene="hcr"
                     /locus_tag="OO161_000144"
     CDS             22402..23370
                     /gene="hcr"
                     /locus_tag="OO161_000144"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5461352.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            23503..25221
                     /gene="poxB"
                     /locus_tag="OO161_000145"
     CDS             23503..25221
                     /gene="poxB"
                     /locus_tag="OO161_000145"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5461353.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            25258..26259
                     /gene="ltaE"
                     /locus_tag="OO161_000146"
     CDS             25258..26259
                     /gene="ltaE"
                     /locus_tag="OO161_000146"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5461354.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            26270..27700
                     /locus_tag="OO161_000147"
     CDS             26270..27700
                     /locus_tag="OO161_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5461355.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            27799..28812
                     /locus_tag="OO161_000148"
     CDS             27799..28812
                     /locus_tag="OO161_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5461356.1"
                     /translation="MKVLVTGTTSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(28809..29639)
                     /gene="amiD"
                     /locus_tag="OO161_000149"
     CDS             complement(28809..29639)
                     /gene="amiD"
                     /locus_tag="OO161_000149"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5461357.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(29636..29959)
                     /locus_tag="OO161_000150"
     CDS             complement(29636..29959)
                     /locus_tag="OO161_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5461358.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            30085..30600
                     /locus_tag="OO161_000151"
     CDS             30085..30600
                     /locus_tag="OO161_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5461359.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            30818..31546
                     /gene="artP"
                     /locus_tag="OO161_000152"
     CDS             30818..31546
                     /gene="artP"
                     /locus_tag="OO161_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5461360.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            31564..32295
                     /gene="artJ"
                     /locus_tag="OO161_000153"
     CDS             31564..32295
                     /gene="artJ"
                     /locus_tag="OO161_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5461361.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            32302..33018
                     /gene="artQ"
                     /locus_tag="OO161_000154"
     CDS             32302..33018
                     /gene="artQ"
                     /locus_tag="OO161_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5461362.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            33018..33686
                     /gene="artM"
                     /locus_tag="OO161_000155"
     CDS             33018..33686
                     /gene="artM"
                     /locus_tag="OO161_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5461363.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            33977..34708
                     /gene="artJ"
                     /locus_tag="OO161_000156"
     CDS             33977..34708
                     /gene="artJ"
                     /locus_tag="OO161_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5461364.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFANLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(34883..36010)
                     /gene="rlmC"
                     /locus_tag="OO161_000157"
     CDS             complement(34883..36010)
                     /gene="rlmC"
                     /locus_tag="OO161_000157"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5461365.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIACAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(36051..36539)
                     /locus_tag="OO161_000158"
     CDS             complement(36051..36539)
                     /locus_tag="OO161_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5461366.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(36599..37444)
                     /gene="potI"
                     /locus_tag="OO161_000159"
     CDS             complement(36599..37444)
                     /gene="potI"
                     /locus_tag="OO161_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5461367.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGAMPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(37441..38394)
                     /gene="potH"
                     /locus_tag="OO161_000160"
                     /pseudo
     CDS             complement(37441..38394)
                     /gene="potH"
                     /locus_tag="OO161_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
     gene            complement(38404..39537)
                     /gene="potG"
                     /locus_tag="OO161_000161"
     CDS             complement(38404..39537)
                     /gene="potG"
                     /locus_tag="OO161_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5461368.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(39632..40744)
                     /gene="potF"
                     /locus_tag="OO161_000162"
     CDS             complement(39632..40744)
                     /gene="potF"
                     /locus_tag="OO161_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5461369.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(41095..41571)
                     /locus_tag="OO161_000163"
     CDS             complement(41095..41571)
                     /locus_tag="OO161_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5461370.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(41659..42417)
                     /locus_tag="OO161_000164"
     CDS             complement(41659..42417)
                     /locus_tag="OO161_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HCR5461371.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLTRQAAESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEG
                     DFRSNLHRGGAASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPG
                     LEGIEKTTGIDIAGKMIRWIERYATTEYCLKTGG"
     gene            complement(42478..43200)
                     /gene="nfsA"
                     /locus_tag="OO161_000165"
     CDS             complement(42478..43200)
                     /gene="nfsA"
                     /locus_tag="OO161_000165"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5461372.1"
                     /translation="MTPTIELIGGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(43184..43471)
                     /locus_tag="OO161_000166"
     CDS             complement(43184..43471)
                     /locus_tag="OO161_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5461373.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            43631..43888
                     /gene="grxA"
                     /locus_tag="OO161_000167"
     CDS             43631..43888
                     /gene="grxA"
                     /locus_tag="OO161_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5461374.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLPQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(43918..44295)
                     /locus_tag="OO161_000168"
     CDS             complement(43918..44295)
                     /locus_tag="OO161_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5461375.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATYMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            44565..46250
                     /locus_tag="OO161_000169"
     CDS             44565..46250
                     /locus_tag="OO161_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5461376.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLAAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(46486..46704)
                     /locus_tag="OO161_000170"
     CDS             complement(46486..46704)
                     /locus_tag="OO161_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5461377.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(46795..47895)
                     /locus_tag="OO161_000171"
     CDS             complement(46795..47895)
                     /locus_tag="OO161_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCR5461378.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            complement(47892..48377)
                     /locus_tag="OO161_000172"
     CDS             complement(47892..48377)
                     /locus_tag="OO161_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5461379.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     L"
     gene            complement(48374..51452)
                     /locus_tag="OO161_000173"
                     /pseudo
     CDS             complement(48374..51452)
                     /locus_tag="OO161_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            complement(51445..51564)
                     /locus_tag="OO161_000174"
     CDS             complement(51445..51564)
                     /locus_tag="OO161_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR5461380.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            complement(51579..51881)
                     /locus_tag="OO161_000175"
     CDS             complement(51579..51881)
                     /locus_tag="OO161_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5461381.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            complement(51936..52451)
                     /locus_tag="OO161_000176"
     CDS             complement(51936..52451)
                     /locus_tag="OO161_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCR5461382.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            complement(52461..53633)
                     /locus_tag="OO161_000177"
     CDS             complement(52461..53633)
                     /locus_tag="OO161_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCR5461383.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQNPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            complement(53776..54342)
                     /locus_tag="OO161_000178"
     CDS             complement(53776..54342)
                     /locus_tag="OO161_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5461384.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGLRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            54370..>54900
                     /locus_tag="OO161_000179"
     CDS             54370..>54900
                     /locus_tag="OO161_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR5461385.1"
                     /translation="MMPVSHYLCIFLNVGLGEGSALPVGVPVPWPSATPPTGWLKCNG
                     AAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDDGRGVDNGRGLLTLQDGAIVSHNHYW
                     GIWTSRTNDQTLESFTGTTILKQITPLSPAIDFDNYPIPNPAITEGGVVAATTKPAGA
                     NETRPRNVAFNYIVRAA"
BASE COUNT        13578 a        14534 c        13169 g        13619 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccaagt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccatc tttaaagaag agctcgtacg aaagtgcgag
     8701 cttttttttc gcattttatc tgtctgtacg gcagtgaaca tggtgggcgg gtggagtatg
     8761 ttacctgtga atttctaagc tgcctgtacg gcagtgaact caaatactac acgtttagcc
     8821 cgttgttaat aaagcaaaac catcccattc tcctgcagca ccctttttta tccttacctt
     8881 cccgcagcac taattatcaa caacttacca tcatcattaa aaaaagggtc tgaacctcct
     8941 ccaccccata aacaaaaagt ccgtaacctc tttcgagatt acggacttga ccaacctacc
     9001 taacaatcag attaatgcgc tgcttccgcc ttgtgctttt gtgcactctg gaatccgtaa
     9061 gtcagctcat ttttctcttt atccagcgcg acggtgacct gaccgccgtc caccagcgaa
     9121 ccaaacagca gttcgttggc gagcggtttt ttcaggttgt cctggatgac acgcgccatc
     9181 ggacgtgcgc ccattgcccg gtcgtaacct ttctcggcca gccagttacg cgcttcctgg
     9241 ctcacttcca gagaaacacc tttctgatcc agctgaacct gcaactcgac gatgaattta
     9301 tccaccacct gatggatcac gtcagttgac agatgatcga accagataat gttgtcgaga
     9361 cggttacgga attccggtgt aaagatcttc ttgatctctt ccatcgcatc ggtgctgtta
     9421 tcctggtgga taagaccaat ggatttacgc tcagtttcac gtaccccggc gttggtggtc
     9481 atcaccagca ccacgttacg gaagtccgct ttgcgtccgt tgttatcggt cagcgtaccg
     9541 ttgtccatca cctgcaacag aatattgaac acgtccggat gcgctttctc gatttcgtcc
     9601 agcaacagca ccgcatgtgg atgcttgatg accgcatcag tcagcaaacc tccctgatca
     9661 aaaccaacgt atcccggagg cgcaccaatc agacggctga cggtatggcg ttccatatac
     9721 tcggacatat caaagcgcag cagctcaatg cccaacgctt tcgaaagctg taccgtcacc
     9781 tctgttttcc cgaccccggt agggccggca aacaggaacg aaccaaccgg tttatgttcg
     9841 tgacctaaac ctgcacgcgc catcttaatg gcttcagtca gcgcctcaat ggctttatcc
     9901 tgaccgaaga ccagcatttt caggcgatcg ccgaggtttt tcagggtatc gcggtcactc
     9961 tgagaaacac tcttctctgg aatgcgcgca atacgggcca ccacggactc aatatccgcc
    10021 acattaacgg ttttcttgcg tttgcttacc ggcatcaggc gtgcgcgagc gcccgcttcg
    10081 tcgataacgt caatggcttt atccggcaga tgacgatcgt taatgtattt caccgccagc
    10141 tctaccgctg cacgcaccgc ttttgcggta taacgcacgt cgtggtgcgc ttcatacttc
    10201 ggtttcaggc cattgatgat ttgaacagtt tcttcgatcg acggttcagt aatatcaatt
    10261 ttctggaagc gacgcgccag agcacggtct ttctcgaaaa tgttgctgaa ctcctgatag
    10321 gttgtcgaac caattacgcg aattttaccg ctggagagca acggtttgat caggttagcc
    10381 gcatcgacct ggccaccaga cgctgcaccc gcaccgataa tggtgtggat ctcatcaata
    10441 aacaggatgc tgttagtgtc ctgctccagc tgcttgagca acgctttaaa acgtttttca
    10501 aagtcgccgc gatatttagt gcccgctaac agagaaccga tatcgagaga gtaaatcgta
    10561 cagtcagcca tcacttccgg cacatcgccc tgaacaattc gccaggcaag accttccgca
    10621 atcgcggttt taccgacacc agattccccc accagcagcg ggttgttttt acggcgacgg
    10681 cagagaacct gaatagcacg ctccagctcc ttctcacgac caatcagtgg gtcgattccg
    10741 cccacgcgcg caagctgatt caggttcgtc gtgaaattct ccatacgttc ctccccacca
    10801 gcttgttctt cgctgtttgg ctggctgcca ggatcagaag actgtgtcgg ctcgtcttta
    10861 cgcgtgccat gagagataaa gttcaccaca tcgagacggc tgacttcatg tttacgcaac
    10921 agatatgccg cctgcgactc ctgttcgcta aagatagcga ccagaacgtt tgcaccggtt
    10981 acctcattgc gaccggagga ctggacatgg aagaccgcac gttgcagtac acgctgaaaa
    11041 ctcagcgtcg gctgtgtgtc gcgctcctct tcactggcag gcagaacggg tgtggtttgt
    11101 tcaataaagg cttccagttc ctgacggagc gcaaccaaat ccacagaaca cgcttccagc
    11161 gcctcccggg cagatgggtt actgagcagc gccagtaaca agtgctcgac ggtcataaac
    11221 tcatgacggt gctcgcgcgc tctggcgaaa gccatattta aactgagttc cagttcttga
    11281 ttgagcatag gcacctcccc caatttttat gcctgcattc aggctttttc tagcgtacac
    11341 agcaatggat gctcattctc cctcgcgtac ttgttcacca tcgccacttt ggtttctgca
    11401 acctcggcgg taaagactcc gcaaatggcc ttcccctggt agtgaacagc gagcatcaat
    11461 tgcgttgcac gttctacatc ataagaaaag aatttttgta acacgtcaat aacaaactcc
    11521 atcggagtgt aatcatcatt gactaatatc actttataca tagatggcgg ttttagcgcg
    11581 tcgcgaactt tttcttccgc cagttgatca aagtccagcc agtcgttcgt tttacccatt
    11641 gtcagttatc atcttcggtt acggttatcg gcagaacgcc ctgccgctga caagagctta
    11701 tttgcctgac caatctactc cacatgatag ataactatca tctatcgttg gcatcagcga
    11761 catctgtcac attcctgtca atagcgttaa ctgcttcaaa tttttgattc atacctgccc
    11821 cctggcccct gtcaaacgct tgacggctcg ccctaattct ctaaattgta tttctagagt
    11881 tggcgaggtt ttgaacagcc ccctctctga ccccggttta ttccatctta cttgtataag
    11941 atttgcgaag gatgtcgaag catggaaaag ggtactgtta agtggttcaa caatgccaaa
    12001 gggtttggtt tcatctgccc tgaaggcggc ggcgaagata ttttcgctca ttattccacc
    12061 attcagatgg atggttacag aacgctaaaa gctggacaat ccgttcagtt tgatgtccac
    12121 caggggccaa aaggcaatca cgccagtgtt attgtgcccg tcgaagtaga agcggcagtc
    12181 gcatagctct tctgtctcat tgtgtacatc ctaaaggcaa aatgccagcc cgatcggctg
    12241 gcatttttat ctcaaaaatt actctcgtgc cagagcatct actggatcca gtcgtgccgc
    12301 atttcgtgcg ggtaaccatc caaataaaat cccggtggcc gtcgagcaga gaaacgccag
    12361 cagcagtgcc aacggtgaaa aaccaatctc ccagccgggt aagaaaagct gcaaggtgaa
    12421 agcaattaac agtgacagcg ttattcccaa cgcgccaccg accaggcaaa ccagtacggc
    12481 ttcgatcagg aactgttgca aaacatcgct tgctcgcgca cctacagcca tgcgaatgcc
    12541 aatttcccgc gtccgctcgg tcactgacac cagcataata ttcattacac caataccgcc
    12601 caccaccagc gaaatcaccg ccaccagcgt cagaaacagt tgtaaagtac gtgtggtctt
    12661 ttcaacagtt ttcaagacgc cgtccatgtt ccaggtaaag aaatccttct ttccgtggcg
    12721 cagtgaaagt aaacgcgtga gttgctgttc cgcctcggcg ctgtcaaatc cttcttttac
    12781 cctgacagta atggagttca gccacgactg gcccataact cgcccggaca tcgtgctgta
    12841 aggtagccag acacgcagca ctttactgct accaaacatc gactgttttt cttccgccac
    12901 accaatgact ctggcgggca tattgccgac cagaatcacc tcgccaacca catctgcttt
    12961 atgggggaaa agctggcggc gagtattact gtcgagaacc acgacctgcg cacgaccgtt
    13021 cagctgctcc tgattaaagg tgtttccttc actgaaggtc atgccataga cattaaaata
    13081 atcgccgctc acgccattgg cactggcagc aacatcaaca ttgttataac gcaggcgcag
    13141 gttttgcgag acggcaggtg tggctgaggc gacccacggt tgtttttgga tggcgattaa
    13201 gtcgtcgtac ttcagcgcct gctgatattg cggatcgtca tcgccaaaat ctttcccggg
    13261 atagacatca atagtattcg taccaataga acgaatatcc gccagcacca tttgtttggc
    13321 ggcgtcaccc accacgacaa tggaaaccac cgacgcaata ccgataataa tccccagcat
    13381 ggtcagtaaa gtacgcattt tattcgctgc cagcgcccgc catgccatcg tcagcgcctc
    13441 gttaaaaccg ctgacaaact gccgccagcc agacaccgtg ttgacaacag gctccgtccc
    13501 gccagcaaca ttcacttttt caatggcggg aggattgcgc acaatttcgc cgtcgcgaat
    13561 ttcgatcacc cgctcggcct gagcagcgac ctgcggatcg tgggtgacga taatcaccgt
    13621 atgcccgcga tcgcgcagct gatgcaggat cgccatcacc tcttcgccag aatggctgtc
    13681 cagtgcgccg gttggttcat cggcaagaat tacctgaccg ccgttcatca atgcccgcgc
    13741 gatgctgacg cgctgttgct gaccacccga aagctgtgcc ggataatact ctgtacggtc
    13801 ttccagcccc agtcgttgca gtaactcctg ggcgcgaagc agccgctgtt tccgctcaag
    13861 accggcatag acggcgggta cttcaacgtt ctgctcggcg gttaaatgcg aaagtaaatg
    13921 gtaacgctgg aaaataaagc cgaaatgctc gcggcgcagt tgcgccagcg catcggcgtc
    13981 cagtgtggca acatcctgac cggcgacgcg ataggtgccg ctggtggcct tatccagaca
    14041 gccgagaata ttcatcaggg tcgatttacc ggaacctgaa gcgccaacaa tcgctaccat
    14101 ctcaccggca taaatatcga gggtgatgct cttcagcacc tcaacctgct catcaccggc
    14161 aggatagctg cgacgaatat cctttaattc gagcaaaggc gtcattgtgc agctcctggt
    14221 ttggcctcac caatcaccac ttcatcgccc gcttccagcc ctttgacaat ctcaacatcg
    14281 gtatcgttac gtgcgccaat cgtcacttca cgctcgcgtg tttcaccatt acgcaacaat
    14341 ttgactttat aacgattatc gccaaccgga tcgcctaacg ccgacagagg gatcgtcagc
    14401 acatttttca catcggtgag ctgaatatgc acttgcgcag tcatatccag ccgcagcaaa
    14461 ccattggggt tggggacttc aaaacgggcg taatagaaaa tagcgtcgtt aaccttttcc
    14521 ggcgtcggta gtacatcctt gatttgcccc tcgtagcgcg tcagtggatc gccaagcacc
    14581 gtaaaccaag ctttttgccc cggcttcagg tggattacat ccgcttcaga aacctgcgct
    14641 tttaccagca tggtgctcat atctgccagc gtcagaatgt tcggtgcttg ttgtgcggca
    14701 atcaccgtct ggccttgcag agtggtgatt tgcgtgactt ccccggccat cggggcaacg
    14761 atgcgagtgt aatcgagatt ggttttagcc gtatcgagag aagcctgatt gcgcttgatt
    14821 tgcgcgtcaa tggtgccaat ttgcgcctgt ttcacagcca tctccgtcgc ggcggtgtcg
    14881 agctcctgct gtgaaaccgc ctgcgtttgt gccagacgtt gctgacggga atacgtcacc
    14941 cgcgccagtt tcagctccgc ttccgcctgc tgccgctgcg cacgtagctc catcagcgtt
    15001 gcttcgacct ccttaatctg gttttcagcc tgttcaggat caataacccc taaaagctgg
    15061 tcttttttta ctttatcgcc aatcgccacc gacagagttt tcaactgacc gctgacctgc
    15121 gcgcccacat caaccttacg cagcgcgtcc agctttccgg tcgccagcac gctttgctgt
    15181 aaatcaccgg ggcgcacaat cagtgtctga taagtcggca cgggtgcgtt aagaattctc
    15241 cataacgtaa tcagtccggc gatgactatc accagcgcaa taacgtaacg cttcttcacg
    15301 gttttccgct ttttcataaa tattctgaat actccatatg ccaatccggg tcattaactt
    15361 caacgaaata tcaacaaaac cctaatattt acgttagtaa tcacactatt gatgtttggt
    15421 taagatacgc aatggtgaaa tcgacgtcat gtataaccat tgatttcatg aatatgtcgc
    15481 agctaactga acggaccttt acgccatctg aatctctcag cagcctgtca ctttttctta
    15541 gtctggcacg tggacagtgt cggccgggta aattctggca tcgccgtagt tttcgccaga
    15601 aatttttgct gcgctcgttg attatgccgc gtttaagcgt tgagtggatg aacgaacttt
    15661 cccactggcc taatctcaat gtgttgttaa cgcgccagcc gcgactgcct gtgcgtctgc
    15721 atcgccctta ccttgcggcg aatcttagcc gtaagcaatt gctggaggcg ttacgttacc
    15781 attatgcgtt actccgcgga tgtatgtcgg cggaagaatt cagcttatat ttgaataccc
    15841 ccgggctgca actggcgaag ctggaaggca aaaacggcga gcagttcacg cttgagctga
    15901 ccatgatgat ctcaatggat aaagaaggtg acagcacaat cctgtttcgc aacagcgaag
    15961 gtattcctct ggcagagatc acgtttaccc tgtgtgaata tcaggggaaa agaacgatgt
    16021 ttattggcgg actgcaaggc gcaaaatggg aaattccaca tcaggaaatc cagaatgcga
    16081 cgaaagcctg ccacgggcta tttcccaaac gcctcgtgat ggaagcggcc tgtctgtttg
    16141 cccaacgttt gcaggtagag cagattattg ccgtcagcaa tgaaacgcat atttaccgca
    16201 gcctgcgtta tcgcgataaa gaaggcaaga tccacgctga ttacaacgct ttctgggaat
    16261 cggttggcgg cgtatgtgat gctgaacgcc attaccgcct tccagcacag atagcacgaa
    16321 aagagattgc cgaaatcgcc agtaaaaaac gggctgaata ccgtcggcgc tatgagatgc
    16381 tcgacgctat tcagccacaa atggccacga tgtttcgcgg ttaatccgcg cgaccgcgcg
    16441 ccagccacag cacgcgtgag aacatttttt tcagcagcgt cggtacggag tccacaccac
    16501 gacgtcctgc ctctattgcc acttcaatgg caagatcggg tttggaagag cgatggatcg
    16561 ctttcgagat aattttgcgt agattcatcg gtacattttc cgggatttgc gccacgcggt
    16621 ggaacacatc ggaaaatccc tggcgataca taaaatgttc catatccagc gccggtaacg
    16681 ccgttaaatg ttctcgttcg gcttcccgat cgttattcaa caggctgcgt accgtagcgg
    16741 catatttctt ccctgcttca tcgccatcga ccagtacatg ccattcaatc cccattcggc
    16801 gggcaaattt aaccagtggc tttagcccgg actgggcaaa ctcaatgacc ttgatccctt
    16861 cggcatcaaa atgatgtccg cactgacgcg ccagttcatt gataacccag gtttccgttt
    16921 ccccttccac cagcaaccag cagcgtgcaa acagcgatga cggacggtta aaacgaatgt
    16981 gaaaggatat gcgtcggcta tcttcggtac tcaagccact cggccccaga cgccaggcgg
    17041 caacgcgcga ggactcacgt accagtcggc aaacatgctc taccggcgtt aacgaaagca
    17101 actcacccga gttggtggtg gcaatgcgct gcaatggcag aagattcaac agatgccagg
    17161 caactgaaag cataatgggg tgtaaacggg tttctggatc ttcgatcagc aacaatggac
    17221 gcgcgtcttt atccagtctc aatgtgcctt ttgcctgcaa taaagtagca aacaggccga
    17281 gcaaaataac ccgatacgag cgccctccag gtcggtcaat catccggttg atgatgtcca
    17341 gatagcgcca gcttcgttgc tcattgctgg cccgccgccg cattaaacga tatcgcgcct
    17401 gtccggcccc ctgctcagag aaataatgct caagcagctg taccattgcg gaaagtccct
    17461 gacgaatctg cccatcagag agattttgcg gatgtgagga taactcacgg gcgaggaaat
    17521 cgagctggcg cgcggtgact tccacattag ggatatttgg caccgtgccg ttacgaatac
    17581 ggcgcataaa acgggcatca cgcaagcgca gcaccggcat taaacgcacc agatggcgtg
    17641 cctgatcgtt aatatcctcg acatcaatcg gatgtccgtc tttatcgaga aaactgcgca
    17701 gtgtcatcac gctgccgtct tccgcactct ccccttccag acgataaaaa atacggtgat
    17761 agccatcggt gcatggcgtc cagcacgctt ccagcggccg ataacggcga acccgatgtc
    17821 ggcctggcag cgattcgcgg aaggtcaaaa taatatgcag atgatgttct cgcccgttga
    17881 tatctcccgg cgggaaccag aaatcgtcgc gctcaaaatg gtagagatct gattctggcg
    17941 atagcagcag ggttaaggcg tccagcaagc tggatttacc ccacgcgttc tccccaatca
    18001 ggacgttgtt ttgttccagc atcaacgaca aacggttgat accgcgaaaa cccacaattt
    18061 caacgcgctc aagaatcata tcccctccag aaatatgtga cttatccttt acgctggcag
    18121 tataacggga aaggccttgc taatacgtga tgacatcaca tttaagcatg gcaaatgtct
    18181 gaaaaatgag cgaataatcg cgttatactc cctcttgctg ataagtctgc aaaaacaaca
    18241 aaatcaaata attagcatga tatattattt tttattacgc ctaaaggatt aaatcttttg
    18301 cgtgcactac actgtcaatc agacaaatga ctatatgggg caacccgctg ttgccctaaa
    18361 tcaaatttac tggatttatt tatctggtga atgactcgcc ttcgttttta aaatagccac
    18421 gcattatttc cgtcgtcatt tttttacgac gaccctataa aacgaccata tttttcacag
    18481 ggtcaatttt taattgaggt ggatatgttc agaaaattag cagctgaatg ttttggtact
    18541 ttctggcttg tttttggtgg ctgtggtagt gctgtactgg ccgcaggctt cccggaatta
    18601 ggcattggtt ttgccggcgt ggcgttggcg ttcggtctga ccgttctgac gatggccttt
    18661 gctgttagtc atatttctgg tggtcatttt aacccggcgg tcactattgg tttatgggct
    18721 ggcggacgtt ttccggcaaa agaagtcgtt ggctacgtaa ttgcccaggt tgtcggcggt
    18781 attgttgcag cggcgctgct gtatttaatt gccagtggta aaacgggttt tgacgcggca
    18841 gccagcggtt ttgcttctaa cggttatggc gagcattcac caggcggtta ttccatgctt
    18901 tccgcgctgg tagttgaact ggtattgagt gcaggtttcc tgttggtgat ccacggcgca
    18961 accgccaaat tcgcgccggc aggttttgcg ccgatcgcta ttggtctggc cttaaccctg
    19021 attcacttaa ttagtattcc ggtgactaac acttctgtta acccggcgcg cagcaccgcg
    19081 gttgctatct tccagggcgg ctgggcatta gaacaactgt ggttcttctg ggtggtgcca
    19141 attgtcggcg gcattatcgg tggtctgatt taccggaccc tgctggaaaa gcgtgattaa
    19201 ttaacgcaga aggcctggcg taatgtcagg ccttttttta agatgttgtt atatgttgat
    19261 tcatcatctt gttgcgctcc tttcatgagc taagccacat attgccactg gcgcaaggag
    19321 cgcgcagggg gcggccaatc gccgccgccc cctgcccccc cgggctctgg cgaacaaaat
    19381 cgccgctgcg cggtgccctc gacttatccc ttacggctac cgggtcgggc gcgaggtaac
    19441 atccctgtaa aacgcgccct cagcccacat ccatgtgggc tgccccggcc ttcagggaac
    19501 gcctcggcga ttttgacgcc accaacatcg gtgcagtttg taattaaagc gcaagaatat
    19561 ctttaaatga aaacgaatac tctactgctc cttatgcata acatttcctt ttaattgcaa
    19621 ctttactcgt ccttccgctt tcagtagtgt gtcatcgcgc atttcgactt catctttgca
    19681 aggactgggt tttcatgttt tctgggctgt taatcattct ggttcccctg attgtgggtt
    19741 acctcattcc gcttcgccaa caagcttcgt taaaagttat taatcagcta ttaagctgga
    19801 tggtttacct tattctcttt tttatgggta tcagtctggc gtttctcgat aacctcgcca
    19861 gtaacctgtt ggcgattctg cattattctg ccgtcagtat taccgttatt ttactgtgta
    19921 atattgccgc cctgatgtgg ctggagcgag gcctgccgtg gcgcaaccac catcagcaag
    19981 aaaaactccc gtcgcgtatt gcgatggcgc tggagtcgct aaaactgtgc ggcgtagtag
    20041 tgattggttt tgccattggt ctgagtggac tggctttctt acaacacgcg accgaagcca
    20101 gtgaatacac gttaattttg ctacttttcc tcgttggtat tcagttgcgc aataatggca
    20161 tgaccttaaa gcagattgtc cttaatcgcc ggggaatgat tgtcgccgtg gtggtggttg
    20221 tcagttcatt aattggtggt ttaattaacg cctttattct tgatctcccc atcaataccg
    20281 cgctggcaat ggcctccggt ttcggctggt attctctttc cggtatttta ttgaccgaat
    20341 cttttggtcc ggtaatcggg agcgcggcgt tttttaatga tctggcccgt gaactgattg
    20401 ctattatgtt gatccctggg ctgattcgcc gcagccgctc tactgcactg ggcttatgcg
    20461 gtgccacatc aatggatttc accctgcccg ttcttcaacg tactggcggg ctggatatgg
    20521 tcccggcggc aattgttcac ggttttattc ttagcctgtt agtgccgatc ctcatcgcct
    20581 ttttctctgc gtaatacctc tctggcggta gatccctgcc gccaaaattg cgctaaatca
    20641 atctccctta aagttgcatg aaaaatccct tttatccccg cgttaagcgt cttaacctta
    20701 aacatgtata ttaaatataa ctttaaaagg tgtgatcatg ttttgtgtgc aatgtgaaca
    20761 aactatccgt actccggcag gaaacggctg ctcatacgcg caggggatgt gtggtaaaac
    20821 ggcggaaact tctgaccttc aggatttact catcgcggcg ctgcaagggc tttctgcctg
    20881 ggcggtaaaa gcgcgtgaat acggcatcat caaccacgat gttgacagct tcgcgccacg
    20941 tgcctttttc tcaaccctga ccaacgttaa cttcgattct cctcgtattg tcggctacgc
    21001 tcgtgaagcg attgccttgc gcgaggcgct gaaagcacaa tgcctggctg tagatgccaa
    21061 cgcgcgcgtc gataacccga tggctgacct gcaactggtg agcgatgatc tcggcgagct
    21121 gcaacgtcag gcagcagaat ttacccctaa caaagataaa gcggcgattg gcgaaaacat
    21181 tctcggcctg cgtctgctgt gcctgtatgg cctgaaaggt gcggcggcct atatggaaca
    21241 cgcgcatgtt ctcggtcaat acgacaacga tatttatgcc cagtaccata aaatcatggc
    21301 gtggctgggg acttggcctg ccgatatgaa cgcgcttctt gagtgttcaa tggaaatcgg
    21361 ccagatgaac ttcaaagtga tgagcattct ggatgcaggc gaaaccggta aatacggtca
    21421 cccgacgcca acccaggtca acgtcaaagc gacggcgggt aaatgcattc tgatttccgg
    21481 tcacgatctc aaagatctct acaacctgct ggaacagacc gaaggcacgg gcgttaatgt
    21541 ctacacccac ggcgaaatgc tgcctgcgca tggctacccg gagctgcgta aattcaagca
    21601 tctggtcggt aactacggca gcggctggca gaatcagcaa gtggagttcg ctcgtttccc
    21661 tggccccatc gtgatgacct cgaactgcat catcgaccca accgtaagcg cttatgacga
    21721 tcgtatctgg acccgcagca ttgttggctg gcctggcgtg cgtcatctgg acggtgatga
    21781 tttctctgcg gttatcaccc aggcgcaaca gatggcaggc ttcccgtaca gcgaaattcc
    21841 gcaccttatc accgtgggtt ttggtcgcca gacgctgctt ggcgctgctg atacgctgat
    21901 tgatctggtg agccgtgaaa aactgcgtca tatcttcctg cttggtggct gtgacggcgc
    21961 acgcggcgag cgccactact tcaccgattt cgccaccatc gtgccggatg actgcctgat
    22021 cctgaccctc gcctgtggta aatatcgctt taacaaactt gagtttggcg atatcgaagg
    22081 tctgccgcgt ctggtagatg caggtcaatg taacgatgct tactcagcga ttattctggc
    22141 tgtcactctg gcagagaaac tgggctgtgg tgtgaacgat ctgccgctgt cgctggtgct
    22201 ctcctggttt gaacagaaag cgatcgtcat tctgctgacg ctgctttctc tgggcgtgaa
    22261 aaatatcgtc accggcccga ctgcacctgg tttcctgaca ccagacctgc tggcggtgct
    22321 gaacgagaaa ttcggcctgc gttctatcac cactgttgaa gaagacatga agcaactgtt
    22381 gagcgcgtaa ggagtttgtc gatgacgatg ccaacgaatc aatgcccgtg gcggatgcag
    22441 gttcatcaca ttacgcaaga aacgccggat gtgtggacga tttccctgat ttgccacgat
    22501 tactacccat atcgcgccgg gcaatatgca ctggtcagcg tgcgaaactc agcggaaacg
    22561 ctgcgtgctt acaccatttc ctccacgcca ggcgtgagtg aatatatcac cctgaccgtg
    22621 cggcggattg atgacggtgt cggctcccag tggctgacgc gcgatgtaaa acgcggtgat
    22681 tatctctggc tttcggacgc gatgggggaa tttacctgcg acgataaagc agaagataaa
    22741 ttcctgttac tggcggcagg ctgcggcgtc acgccgatta tgtcgatgcg tcgctggctt
    22801 gcgaagaacc gtccacaggc cgatgtgcgg gtgatctaca acgtgcgtac gccgcaggat
    22861 gttattttcg ccgatgagtg gcgtaactat ccggtaacgc tggtggcaga aaataacgtt
    22921 accgaaggct ttatcgctgg tcgtctcact cgcgaactgc tggcaggtgt acctgactta
    22981 gcttcacgta ccgtgatgac ctgcggcccg gctccgtata tggattgggt agagcaggaa
    23041 gtgaaagcgc tcggcgtgac gcgtttcttt aaagagaaat tcttcacccc agtagcagaa
    23101 gcggcgacca gcggtctgaa attcaccaaa ctgcaaccgg cacgagaatt ttacgccccg
    23161 gttggcacca cgctactgga ggcgctggaa agcaataacg ttccggttgt cgctgcctgc
    23221 cgtgcgggtg tttgcggctg ctgtaagaca aaagtggttt ccggtgaata tacggtgagc
    23281 agtacaatga cgctgaccga cgccgaaatc gctgaaggtt acgtactggc ctgctcctgc
    23341 catccgcagg gggatttggt tctcgcataa tcgccttatg cccgatgata ttcctttcat
    23401 cgggctattt aaccgttagt gcctcctttc tctcccatcc cttccccctc cgtcagatga
    23461 actaaacttg ttaccgttat cacattcagg agatggagaa ccatgaaaca aacggttgca
    23521 gcttatatcg ccaaaacact cgaatcggca ggggtgaaac gcatctgggg agtcacaggc
    23581 gactctctga atggtcttag tgacagtctt aatcgcatgg gcaccatcga gtggatgtcc
    23641 acccgccacg aagaagtggc ggcctttgcc gctggcgctg aagcacaact tagcggagaa
    23701 ctggcggtct gcgccggatc gtgcggcccc ggcaacctgc acttaatcaa cggcctgttt
    23761 gattgccacc gcaatcacgt tccggtactg gcgattgccg ctcatattcc ctccagcgaa
    23821 attggcagcg gctatttcca ggaaacccac ccacaagagc tattccgcga atgtagtcac
    23881 tattgcgagc tggtttccag cccggagcag atcccacaag tactggcgat tgccatgcgc
    23941 aaagcggtgc ttaaccgtgg cgtttcggtt gtcgtgttac caggcgacgt ggcgttaaaa
    24001 cctgcgccag aaggggcaac cacgcactgg tatcatgcgc cacaaccagt cgtgacgccg
    24061 gaagaagaag agttacgcaa actggcgcaa ctgctgcgtt attccagcaa tatcgccctg
    24121 atgtgtggca gcggctgcgc gggggcgcat aaagagttag ttgagtttgc cgggaaaatt
    24181 aaagcgccta ttgttcatgc cctgcgcggt aaagaacatg tcgaatacga taatccgtat
    24241 gatgttggaa tgaccgggtt aatcggcttc tcatcaggtt tccataccat gatgaacgcc
    24301 gacacgttag tgctactcgg cacgcaattt ccctaccgcg ccttctaccc gaccgatgcc
    24361 aaaatcattc agattgatat caacccagcc agcatcggcg ctcacagcaa ggtggatatg
    24421 gcactggtcg gcgatatcaa gtcgactctg cgtgcattgc ttccattggt ggaagaaaaa
    24481 gccgatcgca agtttctgga taaagcgctg gaagattacc gcgacgcccg caaagggctg
    24541 gacgatttag ctaaaccaag cgagaaagcc attcacccgc aatatctggc gcagcaaatt
    24601 agtcattttg ccgccgatga cgctattttc acctgtgacg ttggtacgcc aacggtgtgg
    24661 gcggcacgtt atctaaaaat gaacggcaag cgtcgcctgt taggttcgtt taaccacggt
    24721 tcgatggcta acgccatgcc gcaggcgctg ggtgcgcagg cgacagagcc agaacgtcag
    24781 gtggtcgcca tgtgcggcga tggcggtttt agcatgttga tgggcgattt cctctcagta
    24841 gtgcagatga aactgccagt gaaaattgtc gtctttaaca acagcgtgct gggctttgtg
    24901 gcgatggaga tgaaagctgg tggctatttg actgacggca ccgaactaca cgacacaaac
    24961 tttgcccgca ttgccgaagc gtgcggcatt acgggtatcc gtgtagaaaa agcgtctgaa
    25021 gttgatgaag ccctgcaacg cgccttctcc atcgacggtc cggtgctggt ggatgtggtg
    25081 gtcgccaaag aagagttagc cattccaccg cagatcaaac tcgaacaggc caaaggattt
    25141 agcttgtata tgttgcgcgc aatcatcagc ggacgcggcg atgaagtgat cgaactggcg
    25201 aaaacgaact ggctaaggta aaaagggtgg catttcccgt cataataagg acatgccatg
    25261 attgatttac gcagtgatac cgttacccga ccgagccgcg ccatgctcga agcgatgatg
    25321 gccgccccgg ttggggacga cgtttacgga gacgacccta ccgttaatgc tctgcaggac
    25381 tatgcagcag agctttccgg taaagaagcc gccatttttc tgcctaccgg cactcaggcc
    25441 aacctggtcg ctctgctcag tcactgcgaa cgtggcgaag agtatattgt cggtcaggcc
    25501 gcgcataact atctgtttga agccggtggc gcagcggtgc tgggcagtat tcagccgcaa
    25561 cccatcgacg cggctgccga cggcacgcta ccgctggata aagtggcgat gaaaatcaaa
    25621 cccgacgata tccatttcgc ccgcaccaaa ttactcagtc tggaaaacac ccacaacggc
    25681 aaagtgctgc cgcgtgaata cctgaaagaa gcatgggaat ttacccgcga gcgcaatctg
    25741 gcgctgcatg ttgacggtgc gcgcatcttt aatgccgtgg tggcttacgg ctgcgaactg
    25801 aaagagatca cgcaatattg tgattcgttc accatttgcc tgtcgaaagg tcttgggacg
    25861 ccagtcggtt cattactcgt cggtaatcgt gattacatta aacgtgccat ccgctggcgg
    25921 aaaatggccg gtggcgggat gcgtcagtcc gggattctgg ctgccgccgg gatgtatgcc
    25981 ctgaaaaata acgtcgcacg gttgcaggaa gatcacgaca acgccgcctg gatggcggag
    26041 caactgcgtg aagcaggcgc ggatgtgatg cgtcaggaca ctaatatgct gtttgttcgc
    26101 gtcggggaag aaaatgctgc cgcgttaggc gaatacatga aagtgagaaa cgtgctgatt
    26161 aacgcctcgc cgattgtccg cctggtgacg catcttgacg tctcgcgcga acaactggcg
    26221 gaagtcgccg cccactggcg cgcattcctg gcgcgttaag gagagagacg tgccgcaacg
    26281 cattttagtt ctcggtgcca gtggctacat tggtcagcat ctggtgcgca cactcagcca
    26341 gcaagggcat cagatcctgg cggcagcacg tcatgtcgac aggcttgcaa agctgcaact
    26401 ggcaaacgtc agttgccata aagtcgatct cagctggccg gataaccttc cggccctgtt
    26461 gcaggatatc gataccgtct attttctggt gcacagcatg ggcgaaggcg gcgattttat
    26521 cgctcaggag cgccaggtgg ctctcaacgt ccgcgatgcg ctacgtgaag taccagttaa
    26581 gcaattaatc tttctcagtt cgttgcaggc cccgccacat gagcagtcgg atcatctgcg
    26641 tgctcgtcag gctacggcgg acattcttcg tgaagcgaat gtacctgtga ccgaacttcg
    26701 ggccggaatt atcgttggcg caggttcagc ggcgttcgaa gtcatgcgcg atatggtcta
    26761 caacctgcca gtgttaacgc cgccacgctg ggtacgttca cgcaccacgc ccatcgcgct
    26821 ggaaaacttg ctgcactatc tggtggcgtt gttagatcat ccggccagcg aacaccgcat
    26881 cttcgaagcc gccggaccag aggtgctcag ttatcagcaa cagtttgaac attttatggc
    26941 ggtgagcggt aagcgccgct ggttgatccc catccccctc cccacccgct ggatttcggt
    27001 gtggtttctc aatgtgatta cttccgtacc gcccaccacc gccagggcgt tgattcaggg
    27061 gctgaaacac gatctgctgg cggatgatac cgcgctacgt gcactcatcc cacaacggct
    27121 gatcgctttc gatgacgcgg tacgtagcac gttgaaagag gaggaaaaac tggtcaactc
    27181 cagcgactgg ggctacgacg ctcaggcctt tgcccgctgg cgaccggagt acggttattt
    27241 tgccaaacag gcggggttta ccgttaaaac gtccgccagc cttgctgctt tatggcaggt
    27301 agtgaaccaa atcggcggta aagagcgtta tttctttggc aatattttgt ggcagacacg
    27361 ggcgttgatg gaccgcgcga tcggtcataa gctggcgaaa ggccgcccgg agcgcgaata
    27421 tttacagact ggcgatgcgg tagatagctg gaaagtgatt gtcgttgaac cggaaaaaca
    27481 acttacgttg ttatttggca tgaaagcgcc ggggctggga cgactgtgtt ttagcctgga
    27541 agataaaggc gactatcgta ctatcgatgt ccgcgctttc tggcatccgc acggtatgcc
    27601 ggggctgttt tactggttat tgatgatccc cgcgcatctg tttatttttc gcggaatggc
    27661 aaaacaaatc gccagactgg cagaacaaag cacagattaa tcgacaaaac ctgcgttatt
    27721 ctttcatgtt tcccattgca tcatggggga aatcacggaa gaatgcgcac gttattttct
    27781 caacaacagt ggattgatat gaaggtactg gttaccggca ccaccagcgg cttaggtcga
    27841 aacgcggtag agtttttatg ccagaaaggc atcagcgtgc gagcgaccgg tcgcaacgag
    27901 gcaatgggca aattgctgga aaaaatgggc gcagagtttg ttccggcgga tctgaccgag
    27961 ctggtttcat cacaagctaa agtgatgctc gcgggcattg atacgctgtg gcactgctcc
    28021 agctttacct caccctgggg gacacaacag gctttcgatc tggctaacgt tcgcgccact
    28081 cgccgcctgg gtgaatgggc tgtcgcctgg ggtgtgcgta actttattca tatctcttcc
    28141 ccctccctgt acttcgatta tcaccaccat cgcgatatta aagaagattt tcgccctcac
    28201 cgcttcgcca acgagtttgc ccgcagcaaa gcagccagcg aagaagtgat caatatgttt
    28261 tcgcaggcga atccacaaac gcgctttact attctgcgcc cacaaagtct gttcggaccg
    28321 cacgataaag tctttattcc ccgtctggcg catatgatgc accactacgg cagtattctg
    28381 ttaccgcatg gcggcagtgc gctggtcgat atgacctact atgaaaatgc cgtgcacgca
    28441 atgtggctgg caagccagga agcctgcgat aagctacctt ccgggcgtgt gtacaacatc
    28501 accaacggcg agcatcgcac actgtgcagc atcgtgcaga agctgatcga cgagttgaat
    28561 attgactgtc gtattcgttc cgtcccctac ccgatgctgg atatgatcgc ccgcagcatg
    28621 gagcgtttag gccgcaagtc agcaaaagag ccgccgctga cccactacgg cgtctccaag
    28681 ttgaattttg actttacgct ggatattacg cgtgcgcagg aagagttagg ttatcagccg
    28741 gtcatcaccc tggatgaagg tatcgagaaa actgccgcct ggctacgcga ccacggaaaa
    28801 ctgccgcgct aatcctgccc gtatttctcc agcaatgctt cggcaatcgc ctgagtttct
    28861 gcatccgctt cgccgttata taacgtcggg cggaaatgca tctggaatgc cataatcacg
    28921 cgccgctgct cgcgcggtgt catatcaggt ttaacgtcat aaccgtagcg cgccaaaagc
    28981 tccagcaatg acgcggtatc taccggtgtg tgcggcgcgc gcccggcaag gtaaaagtta
    29041 acccgctgcg catccggcca agcaccaatc ccctgctgcg ccagttgctg ccagggaaat
    29101 aatggccccg gatcgtcttt gcgctgcggt gcgatatccg catgtgccac tacgttttcc
    29161 ggcttgatgt gataacgggc aataatatct ttcgccagtg gaataagcgc ctgaatctgt
    29221 gccggttcaa acggggcaaa atatttcact ccggccgatt tttgccagcc acggttttcc
    29281 agctcaatgc caatagaggt gtcgttaagg cgcgttgccc cgcgccaggc gctaatcccc
    29341 gcatgccagg ccagttcttg ttccggcacc agttgccaga tgcgcggttt accgttgtat
    29401 cgcggtggta ccgcagggac cagataatgc gagctgacct gcttatcggt cagtgtcgcc
    29461 agcgagctat caaaatcatc tgcggtgtag tggatcacca gcactttaat gcgcggatac
    29521 gccgcctgcg cctggcgtcg ggtatcaagc tgatatccct ctttctcgac aatgcctttt
    29581 tcgcctgcac accctgccaa taacagagcg gcagcgacca gccagaagac tcttctcatc
    29641 gacgcgtttt caccgccgta ccgctgacgc taaccatcag catactgccg ttttgcccga
    29701 ccgtttcgta gtcgatatca ataccgacga cggcatcggc ccccagcgcc cgcgcctggg
    29761 agcctaattc ctcaaaggcg atctcccgtg ctttacgcag ttctttttca tacgcaccgg
    29821 aacgtccgcc aacgatatcg cggataccgg caaagaaatc acggaaaata ttggcaccta
    29881 aaatcgcttc gccggtcacc acaccgcaat attcaacgat ggtctggcct tccagagttg
    29941 gggttgtaga aaattgcatg atcgatctcc ttgtttgcta agccttcgat ctcaaaagca
    30001 ttatcagact gatacgctat tattgaaagg atatcattat tatcgtagaa cgctttcaga
    30061 gcgatcgctt ataaggaaat cattatgcgc tacagcaaat tgacaatgct tatcccctgc
    30121 gcactgctcc tcagtgcctg caccacagtc actccagctt ataaagataa cggcacacgc
    30181 agtggtcctt gcgttgaagg aggcccgggt aacgtggccc agcaatttta tgactaccgc
    30241 attttgcacc gcagcaacga catcaccgcc ttgcgccctt atctgagcga caaactggcg
    30301 acactgctta gcgatgcctc ccgggataat aaccatcggg aactactgac caacgatcca
    30361 ttctccagtc gcacaacctt gccagagagc gcccgtgttg ccagtgcctc aacaatcccg
    30421 aatcgtgatg cgcgaaatat tcctctgcgt gtggatctaa aacagggtga tcagagctgg
    30481 caggatgaag tgttaatgat tcaggaaggc cagtgttggg tgatcgacga tgtacgctac
    30541 ttaggcggca gcgttcatgc cacggcgggt acgctccgtc agtccatcga gaatcgctaa
    30601 cgccttgaac aaccaataaa ccccgtaaat tgtccggcat ttcgtgagga atgccgacat
    30661 ttatgctcgc cgaccaccgc ccccgttatt ttgtgctatg tttattgaat aatgcgcttt
    30721 gcttttaact tttaaagcag aaatattgca taattattct gtcaacggta ctatcttcga
    30781 cctcaatagc tatcagactg ccagtatacg agtgtcaatg agtattcaat taaacggcat
    30841 taattgcttc tacggcgcgc atcaggcgct gttcgatatc acgctggatt gcccacaggg
    30901 cgaaacgctg gtgttacttg gccccagcgg cgcgggtaaa agctcgctgc tgcgtgtact
    30961 caatctgctt gagatgccgc gctccggtac gctcaacatt gcaggcaacc atttcgattt
    31021 caccaaaaca ccctctgaca aagcgattcg cgatttgcgt cgtaacgttg gcatggtgtt
    31081 tcagcaatac aacctgtggc cgcatctgac cgtgcagcaa aacctgattg aagcgccctg
    31141 ccgtgtactg gggttgagta aagatcaggc gctggcccgt gcagaaaaac tgctggaacg
    31201 tctgcgtctc aaaccttata gcgatcgtta cccgctgcat ctttctggtg gtcagcagca
    31261 gcgtgttgct attgcccgtg cgttgatgat ggaaccgcag gtactgctgt tcgatgaacc
    31321 gaccgccgca ctggacccgg aaattacggc acaaatcgtc agcatcattc gtgagctggc
    31381 agaaacgaat attacccagg tgatcgtcac ccacgaagtt gaagtggcgc gtaaaaccgc
    31441 cagccgagtg gtgtatatgg aaaatggtca tatcgtagaa caaggcgacg cgagctgctt
    31501 taccgagccg caaaccgaag catttaaaaa ctatctctct cactaagatt cgggataaca
    31561 acaatgaaaa aagttctgat tgccgcgtta attgcaggtt ttagtctttc cgccacagct
    31621 gccgaaacca ttcgttttgc taccgaagca tcctatcctc cgtttgaatc gattgatgca
    31681 aacaaccaga tcgttggttt tgacgtcgac ctggcacaag cgctgtgtaa agagattgat
    31741 gcaacctgca ctttctctaa ccaggcgttt gacagcctga tcccaagcct gaaattccgt
    31801 cgcgtagaag ccgtgatggc gggcatggat atcactccgg agcgtgaaaa gcaggtgctg
    31861 tttaccaccc cgtactatga caactctgcc ctgtttgtgg gtcagcaagg caaatacacc
    31921 agtgttgatc agctgaaagg caaaaaagtc ggcgtacaga acgggacgac acaccagaaa
    31981 ttcattatgg ataagcaccc ggaaatcacc accgttccgt atgacagcta ccagaacgca
    32041 aaactggatc tgcaaaacgg gcgtatcgac ggcgtcttcg gtgacaccgc agtggtcact
    32101 gagtggctga aagataaccc gaaactggcg gcggtgggcg acaaagtgac cgataaagat
    32161 tacttcggca ctggcctcgg catcgcggta cgtcagggca acactgagct gcagcagaaa
    32221 ctcaacactg cgctggaaaa agtgaagaaa gatggcactt acgaaaccat ctacaacaaa
    32281 tggttccaga agtaattcct gatgaatgaa ttttttcctt tagcaagcgc cgccgggatg
    32341 accgtcggcc ttgccgtttg tgcattgatt gtcgggctgg cgctggcgat gttctttgcg
    32401 gtatgggagt cggaaaaatg gcgtcctgtc gcgtgggcag gttcagcgct ggtaaccatt
    32461 ctgcgtggcc tgccagaaat tctggtggtg ctgtttatct attttggctc ctcgcagctg
    32521 ctgctgacgc tttcggatgg cttcactatc aatcttgggt tcgtgcagat cccagtgcag
    32581 atggacattg agaacttcga cgttagcccg ttcctttgtg gtgtcatcgc tctgtcactg
    32641 ctgtatgccg cctatgcctc gcaaacgctt cggggcgcgt tgaaagcggt gccggtgggt
    32701 cagtgggagt ctggtcaggc gctggggctg tcgaaatcgg ctatcttttt ccgtctggtg
    32761 atgccgcaga tgtggcgtca tgcgctgcct ggcctcggta accagtggct ggtgctgctg
    32821 aaagataccg cgctggtcag tttgattagt gtgaatgatt taatgctgca aacaaaaagc
    32881 atcgctactc gtacccagga accatttacc tggtacattg tggcggcggc gatttacctg
    32941 gtgatcaccc tgctcagtca gtacattctc aaacgcattg acctgcgcgc gacacgtttt
    33001 gagcggaggc ccagctaatg tttgagtatt tacccgaact gatgaaaggg ctacacacca
    33061 gcctgacgct aaccgttgcc tcgctgattg tggcactgat tctggcattg atttttacca
    33121 tcatcctgac gctgaaaacg ccggtgctgg tgtggctggt gcggggttat atcacgctgt
    33181 ttaccggtac gccgctgctg gtgcagatct tcctgattta ttacgggccg ggccagtttc
    33241 cgactttgca ggagtatccg gcactgtggc atttgttgtc agaaccgtgg ttatgtgcgc
    33301 tgattgcgtt atcgctgaac agtgcggcgt ataccacgca gctgttttac ggtgcaattc
    33361 gtgcgatccc agaaggtcag tggcagtcct gtagcgccct gggaatgagc aaaaaagata
    33421 cgctggcgat cctgctgccg tatgccttta aacgctcgct ctcttcttat tccaacgaag
    33481 tggtgctggt gttcaaaagt acctctctgg cttacaccat tacgctgatg gaagtgatgg
    33541 gatacagcca gttgttgtac ggacgcacct acgatgtaat ggtgttcggt gcggcaggga
    33601 ttatttacct ggtcgtcaac ggcctgctga cgctgatgat gcgtctgatc gagcgcaaag
    33661 cactggcatt cgaacggcgg aattaatgta gctgcgtaca ttcgccgggg ggtttgtagg
    33721 tctgataaga cgcgccagcg tcgcatcaga catcagcacg gtgcctgtgc cggatgcggc
    33781 gtggacgcct tatccggcct acatagtgca taaattctta taatgaagac ggacaactca
    33841 ctaagttatc cgtctttttt tatttcattt aaattattta atcatattta ttgcatataa
    33901 attcacttga tgacattatt atcccatgcc gcagacacgg ccaaaaatca taagattgac
    33961 agacgggagt tccatcatga aaaagttagt tcttgccgct ttacttgctt cctttacttt
    34021 cggtgcttct gccgcagaga aaatcaattt tggcgtttca gccacctatc caccctttga
    34081 atctataggt gctaataatg agattgtcgg ctttgatatc gatctggcaa aagccttgtg
    34141 caaacaaatg caggcagaat gtacttttac taatcacgcg ttcgacagcc tgatcccgtc
    34201 actgaaattc agaaaatatg acgccgtaat ctccggtatg gatatcacgc ccgagcgtag
    34261 caagcaggta tcttttacca cgccctacta tgaaaactca gccgtcgtga ttgccaaaaa
    34321 agatacctac aaaacgtttg ccaatctgaa aggcaaacgt attgggatgg aaaacggcac
    34381 cacgcaccag aaatatattc aggatcagca ccccgaagtg aaaactgtct cctatgacag
    34441 ttatcagaat gcctttatcg atctgaaaaa tggtcgtatt gatggggtat ttggtgacac
    34501 agcggtggta aacgaatggc tgaaaaccaa tccgcaactg ggcgttgcta ctgaaaaagt
    34561 gaccgatccg caatacttcg gcaccggtct gggcatcgct gtacgtccgg ataacaaagc
    34621 tctgctggaa aaactgaata acgcgctggc agcaattaaa gctgacggta catatcagaa
    34681 aatcaatgac cagtggttcc cgcagtaatt gagactgatg acaaaagtaa gattgcctga
    34741 tgcgctgcgc ttatctggcc cacatcaact ctgcaatgta ttgatttagc atgattttgt
    34801 aggccggata aggcgtacac gccgcatccg gcatgaacaa agcgcacctg atcagcaatc
    34861 tgaagccgca cctgcggctt ttttattgct tcaccagcag cgtcagcact tcatagtgcg
    34921 cggtgtgcgg gaacatatca aaaagctgta cccgttcgat gcgaaaccca ggcagttcgc
    34981 ggatatcttt cgccatagtt tgggcgttac agctggagta gatgataaaa cgcggtgcca
    35041 tcgttgagag ataatcacac agcggtttac caatgccgcg gcgcggcggg ttaaccagca
    35101 ccagctccgg cacctccccc tgagcagtgg caaactgagt ggagtccagc gcctgaaatt
    35161 gtaaacgagt taagcccagt tcagcggctg actgctttgc acaggcaatg gcctctggcg
    35221 caatttcgat cccggttaac tgcatgtcag gcgtcgcgca gtgtaaacca aagcccccca
    35281 caccgcagaa cagatcccac atatgtttaa ctggcagctg ccgtacccag tcgcgcgcgg
    35341 tagcgtacag ctggctggcg accgccggat tagtctggaa gaaactttgc ggacggatcc
    35401 acagcggtac gtcattaaaa cgctctgcca gcgcctgttg ttcagtcagg tagatctccg
    35461 tctccccttc cataatcgcc atatgtaccg gctgaatatt gacggtaata actttcagtt
    35521 gcggtagttg ttcctgtaac cacggcagcg ccttacgcag ttgtgccagt ttggtttcag
    35581 aacgcagtac aaagcgcagc atcatgccgc catcgctctg gctttcagtc agcagaatgt
    35641 atttcagttc gccacgctta cgcgccacgt tgtagggcgt taaccccgca caggcgataa
    35701 acggttttag cgccgcaaaa acgggcgcaa aagaggcagg ataaagcggg cagtcacaaa
    35761 ggtcttccgg cgtgccatcg cgatgcagca taccgagcag tggtttttca acgctaccac
    35821 tcaccaccat tttggcttta ttacgaaacc cttgttccgg gcctgacacc ggcgcgcacc
    35881 attcctcaac cggaaaatcg gcgagcagat ttttaagatc ggcggtttta gcggagagtt
    35941 gctctggaat cggctgcgtt atccactgac aggaacgaca gcgacccgcg tcgtaaagtg
    36001 cgcactgcat acattgacct tcacatcatc aggggcgacg attatacaca ttattgcaac
    36061 tggaagaacc gccgactggt cgagggaacg aacagcagca tcaggatgag catatccggc
    36121 agcttctgca gcatcaggct atggaagatt tcacgttttg attcaccggg aatgctgaac
    36181 agctccggat aaccataccc cagcgaagcc gcccacaagt aacttgcggc ggtgatttgt
    36241 gtcagcagat atagccagcg cgcccagcga cgccctttca ccagtgaaaa cgcacaccag
    36301 atctcgataa aaaccagcac cagactgctt aaaaatacca gcgttaagct ccaggtttgt
    36361 acgctgcgat gaatgaactc gccaataccg cgcacgccca gcgtattgaa aatcatcagc
    36421 acgtcgaggc cacggatcat aataatggcg agcgccgcta cctgcaccag cgcaggcaca
    36481 ttcaggcgag catgagatga agatgttttc ttaaaaaatc ccaacgtttc gtcttccatg
    36541 aaaacaatgc cgcggcatgc gcggcattat gtagccaggt tggcaaattt tagtgtcttc
    36601 agccatgtct tgcacgctgg atatcgcgta tccgctgttt ttctgcgcga gccatcagat
    36661 accaggcgat aaatccgaca attccgaccg caccgagaat taacgttgca agggcgttga
    36721 tttccggatt cacccccatc cgcacgctgg aaaagaccag catcggtaac gtggtggctc
    36781 ccggcccgga aacaaagctg gcgatcacca gatcatcaag cgacaaagta aaagccagta
    36841 accagccaga aatgatcgca ggcatgatca tcggtagcgt aatgacgaaa aacactttca
    36901 gcggcatcgc accgagatcc atcgctgctt cttctatcga gcgatccagt tcccgcagac
    36961 gcgacgaaat aacgaccgcc acataagccg tacagaaagt gacatgcgcc agccagatgg
    37021 taagcatacc gcggtccgca ggccagccaa tggcatgagc aagcgcgacg aataataaca
    37081 acagcgacaa gcccgtgatg acatctggca ttaccagcgg cgcggtgatc ataaaggcaa
    37141 agccatttga tccgcgaaac ctgccaaagc gcaccagcac caccgccgca atagtcccga
    37201 ggatcgccgc cgccgttgcc gcacaggcag caattgtcag gcttaaacca accgcactca
    37261 tcatcgcgtc atcgcgcaat aactcaccat accagcgcgt tgaccagccg gcccacaccg
    37321 tcaccagctt cgagctgtta aacgaataga taaccagcat cagcattggc gcgtagagaa
    37381 aggtaaagcc cagcagcaaa atcacaatcc gccagggcga acgaactacc ggtaaattat
    37441 tcatccgtgt tctcccacgc ttttttgctg gtgtttgtgg aaccacatta tcggcacaat
    37501 tagcagcagc aacatgatga tcgctaccgc cgcggccacc ggccagtcgc ggttattgaa
    37561 aaactcttgc catagcacgc gcccgatcat gatgctgtcc gggccaccga gcagttccgg
    37621 gatcacaaac tcgcccacag ccgggataaa caccagcatc gatccggcaa taatcccacc
    37681 tttagtcagc ggcacgatca cagtaaagaa cgttttcagc ggtcgtgcac cgagatccag
    37741 cgctgcttcc actagcgaat aatcaatacg aatcaacgcg gtataaatcg gcagcaccat
    37801 aaacggcacg taggcgtaaa caatgccaat ataaactgcc agattggtat gaagaatggt
    37861 cagcggttga tcgataaccc ccagccacag cagaaaatta ttcagcacac cgttgttttt
    37921 taatattccc atccaggcat aaacgcggat cagaaacgag gtccacgacg gcaggatcac
    37981 cagtagtaat aaaatattac gggtcgaagg cttactgtgc gccaccgccc acgccagcgg
    38041 atagccgatc agtaaacagc aaaatgtcta aatcgcagcc acctgtagcg actggagata
    38101 agcatcgaaa tagagcggat cgtcggtcag ttgcagaaaa ttaccgagat taagagtgat
    38161 ggacagttgc ccgtcagccc actccatcag ttcggtataa ggtggaatag cgcgcgccat
    38221 ctccgccagg cttattttaa agacaatcag aaatggcagc agaaacagca agatcaacca
    38281 gatatatggc aacgcaatga ccagtttgcg cccatgcttc atttgcagct gcgacaacca
    38341 cagcttaaat ccgcccggcg gtttcgactg ggcagcaggt tcaagtgtat tcatctttgc
    38401 tccttaaacc gtcagcacca cacagctgtc cacttcccag cacaaacgca cttcgtcgcc
    38461 ccaggtcggt aaccctttac gatgacgatg ggcgttttgt agctgggcgc taatcatctg
    38521 cccacttttc agacgaacgt gatacaccga aagatcgccg agataggcaa tgtgtatcac
    38581 ctcccccacc gcgaagttac aaccattggc gggcggctct tcgcaaagca tgattttttc
    38641 cgggcgcagc gccacatgta ccggcacgtt atcgaccacc gaggcatccg cgtcgacttt
    38701 cagtggatgc accagcccag gcgaataaag cacaagccca tcctcatgac gctctttgag
    38761 tacaccttca aagacattaa ccgaaccgat aaattcagca ctgtagcggg tggttggatg
    38821 ttcgtagatc tcttctggct cgccaatctg gacaaatttc ccgcgattca taatggcgat
    38881 acgtcccgcc atcgtcatcg cctcttcctg atcgtgagtg accatcacac aggtcacgcc
    38941 gacacgctcc agaatatcca ccacttcaag ctgcatccgg tcacgcagct ttttatccag
    39001 cgcgcccatc ggctcgtcga gcagtaatag tttcgggcgc ttggcaaggc ttcgggccag
    39061 cgccacacgc tgtcgctgac caccggaaag ctgatgcggt ttgcgtttgg cgaactcctg
    39121 catgtgcacc aggccgagca tctcatggac ccgactggca atttccgctt tcggtagttt
    39181 gtcctgtttc aggccaaaag cgatgttctg ttccacggtc atatgcggaa acagcgcgta
    39241 agactgaaac atcatattga tggggcgcag gtaaggcggc acctgtgaca aatcgacgcc
    39301 atcgagcatt atctgcccgg cagaaggttg ttcaaaacct gccagcatac gcagcagcgt
    39361 ggacttgcca cagccggaag cgcccagcag cgcgaagatt tcacctttgt agatggtcag
    39421 gctgacatca tcgaccgcat gctgaccatc gtaggatttg gtgaggttgc ggatttctaa
    39481 tagcggcgtc agcgccttac gggttttcgc ctgcgggcga gggatagcgt cattcacggc
    39541 tgctctccgg cattgaatgt gtgcaagcgc accatgatgg tgcgaattgc cggcgggtgt
    39601 ggtgcgcccg cccggcatct aaggctgcgg attattttcc gctcttcact ttggtccacg
    39661 cgcgggtgcg cacacggtcg attttcggat cctgtacttt cagagtgaat agcttcgcac
    39721 gaacgtccgc cggaggataa atacccgggt tctcacggac ttccgcactc accagcggcg
    39781 tggctgcttt attggcgttg gcatagaaca catggtcgga aatatgcgct actacatccg
    39841 ggcgcagcag gtaattcagg aactgatagg cttcgtcttt gtttttggca tccgcaggca
    39901 tggcgaatac atcaaagaac gccatcgccc cttcttttgg aatcgagaac gagacattca
    39961 ctccattctt cgcttccttc gcgcggtttg acgcctgcca gacatcacct gcccagccga
    40021 tagcgacgca aatatcgccg tttgccaggt cgttaatgta ttgagatgaa tggaaataac
    40081 gaatgttcgg gcgcagcttt aacagcagat cggttgccgg tccggtgtaa tcatccgctt
    40141 tagtgctgtt gggatctttg ccgagataat tcaacacggt agcaaaaact tcttctggcg
    40201 catccaggaa agagacaccg cagcttttca gtttttccag attttcaggt ttgaggatca
    40261 agtcccagct atcgacgggc gcgttttcgc ccagcacagc tttaacttta tcaacgttat
    40321 agccaatccc ggtcgtcgcc cacatatagg gcatagcaaa tttattgtcg ggatcgtgtt
    40381 tggcgaccag cttcagcagc tccgggtcga gattcttcca ctccggcaat ttgcttttgt
    40441 ccagcggctg gaaaactccc gcagtcaact ggcgttcaag aaagctggca gatggaacca
    40501 ccaagtcaaa gccggtgcta cccgccatta atttgccttc cagtacttcg ttagagtcga
    40561 aaacatcgta gacgacttta ataccggttt ctttttcaaa attggccacc gtgtcagggg
    40621 cgatataatc agaccagtta taaatgtgga gtgttttttg ttcagccgcg agcgtgccga
    40681 cagagacggc catcagagca cccgcaacca gacccgaaag ccatttttta tttaaggcgg
    40741 tcatatctct ttccttctga aagttcgtta acaaacgaac aaaagttgtc atactaaaag
    40801 atatgcaata aacgtgcata ttttttatcg gcgcggtgta agaaaagaga actctctccc
    40861 ggccagcact gctcgctata taaaagcatc gcaagaataa ctttagccag ggtttgagac
    40921 tatagctcgg attaaaaaac ggtatttaac aaattgttat gcgtatttgc tctatgtgat
    40981 aaagccaaat cggcacaaga aaagtgaaat aatctttaag aaaaggttaa aaacagcaga
    41041 taattttgca gtgcgcagtc actgcaccag cgactgcgtg ctcgaaagga gttatcagtg
    41101 gaggaaatag ttttcggtaa cgttgttttg cccttcatcg tcagtcggta acagcagttg
    41161 atgggcatta gcttcaagaa tgaccatcga aatctgctct tcgctctgac gcacgaacca
    41221 tgcaaactgc tcgtaggtta cgccctgcat aacggataaa gactggcaaa ccaccagctt
    41281 tggcagatta tcatcctgca tatcaagaaa tgctttcacg gtcagcgaac tggcattgat
    41341 ggcagataaa tccgccgcca gcggcaatac ggctgaaggt cggacttccg ccatggcaga
    41401 aaacaggatc gtgttatcga tcagatcgat tttggcatca aagacaccgt cgaaattctg
    41461 catatggggc agatgcagag cctgacagtt atcacattca aaaaaactca tccccaggtc
    41521 atcgagccat tgacgcagcg tatccagacc aggaacgacc agcgatgtca taattttgta
    41581 caacctcttt cgataaaaag accggcacag cttacgcaaa aagcgcaggc aaaaccatga
    41641 tcagtaatgt gattgcgact aaccacccgt tttcaggcaa tattctgtcg tagcgtagcg
    41701 ttcgatccag cggatcattt tacccgcgat gtcgatacca gtggtttttt ctattccttc
    41761 cagccccggc gacgcattca cttccatcac caacggcccg cgattagcac gcagaatatc
    41821 cacaccagca acgtccagcg ccatcgttcg cgcggctttt atcgcgattt cacgctcctg
    41881 tggtgtgata ctggcgacgc ttgccgcgcc gccacgatgc aaattggaac gaaaatcgcc
    41941 ctctttcgcc cgccgttcaa tcgcagcgac cacttcatcg ccaacaacca gacagcggat
    42001 atcgcaccct tgcgcctctt tgatatattc ctgcaccaga atatgcgcgt tcagaccgcg
    42061 gaaagcgtca atcacgcttt ccgccgcctg acgcgtcagc agttgcatgg aacgcaattt
    42121 gtcacgcgcc cgggcaatgg cgaccgactc attgagcgga tagctcccca gcatctcgaa
    42181 ctggcgcagt gccgccgtcc cataaaaggt aatggcggtg ccaatacgcg ggatcactgc
    42241 gtcaaaatgg ggtaacttgc ggcctttgta gtgaatagaa gacgccgcag gatttatgtt
    42301 catgtagcaa gaaagcggat caagaatttc aaccaggtga ccgcgctgta tagcggcttc
    42361 acgcagccgc ttacacgaat agagcgttcc atcccgggac aatatggcaa ttttcaccct
    42421 gcacctctct gtcagaccag gtcaaaagcc cgcgtatcat acatcgacgt ggcggtttta
    42481 gcgcgtcgcc caaccctgtt tgtgcaaata atccagaata aatgggcggc tttctttaat
    42541 gattgttcgg cggatatgat cgctccaggt atcccggcga ttattgctgc cacgggtgag
    42601 gtaatattcc gccagttgct cgtcatactg cgccagtgcg tctttatcca gcggttgata
    42661 gctgttttca tgcaccaaaa tggaggccgg taaacgcggc ttgagatccg gattatccgc
    42721 aggccagcca aggcacagcc caaacagcgg cagaacatgc tgcggtaatt tcagcagttt
    42781 cgtcaccgct tcaatattat tgcgcaggcc gccgatatat accccgccca atcccagcga
    42841 ttccgctgcg gttaatgcat tctgcgccat cattgccgta tcaacgacac cgagcaacag
    42901 ttgttccgcc aggccgagct gagcatccgg acagatctgt aaatggcggt taaagtcggc
    42961 acagaacacc cagaactccg ccgcttgcgc tacgtgtttt tgcccgccgg tcagcgtcac
    43021 cagttcttca cgtaatgctt tgtcggtaat gcgaataatg ctactacact gcaaaaaact
    43081 ggaactggac gtcgcacggg cgctgttaat aatcgcctca cactgcgctt cggaaatggg
    43141 ttcatcagtg aaatggcgaa tggagcgatg gccaccaata agttcaatgg ttggcgtcat
    43201 tatctttttc tctttctgaa cgtgaatatt gcggtggacg gttcatcagc tgtggggcaa
    43261 gacgttttgc cacctgaaga ataaccacca ccgcagcggg aagcatgagc aaaacaccga
    43321 gaaaaatcat cagaatctgc acttctggcc gagaaaatgg ctcaggcagc gacagggagt
    43381 cgcttaccga cagcagcgcc accgccagta gcatcattcc gataaattcc agtatcaaca
    43441 cgcctttagg caatttaccg atcgcgcgca tacgcttccc tctgcaaagt gagccttcag
    43501 tctaaaactt ttcactgtat tgtgtttaac agttatagct tttagcaatt aatgcaacag
    43561 gttaaaccta ctttcagcga atacatttta gcgtgatcat tacaggcata aatctatgag
    43621 gagagaaata atgcaaaccg ttatttttgg tcgttcgggt tgcccttact gtgtgcgtgc
    43681 aaaagatctg gctgagaaat tgagcaatga acgcgatgat tttcagtatc agtatgtaga
    43741 tattcgtgcg gaagggatca ctaaagaaga tctaccacaa aaggcaggta aacccgtaga
    43801 aaccgtgccg cagatttttg tcgatcagca acatatcggc ggctataccg attttgctgc
    43861 atgggtgaaa gaaaatctgg acgcctgatc gtctgacaag ccctcgcgtt gagggcttta
    43921 ctgatttttt ctgtgctgtg gtttaaacaa actactgata aataagaaac acagtgcccc
    43981 cagcgcacac cagaacaccg cgcttagtaa ccatgccagc tcttgccaga atgagcgcgt
    44041 cggtgaaaaa aacagccgca taatgagcat tgaacagggt gccgccagca ttgcgccaaa
    44101 tagaggtttc aggacttctc tacgctgtga aaagaaactg gcaactgctc caggaagaat
    44161 gaaaaacagc aagccgattt caggatgccc ggcagcccga aaagcgcctt tcatgtacgt
    44221 cgccagaaaa aggcacacca caatgaagag gacaaaacag cagattgccc ctgcccaacg
    44281 ttgtttatgt ttcactcgtt cctcctgaca ctgcgtctat cgaacacatt tttcgccagt
    44341 gtggcgttca gtaagataaa gccgcttcgc attccatgct aatataggcc aacgcaattc
    44401 atatagccgt tgatacctaa tgtgattaca ctagtaaaat acattgttac tttactatcg
    44461 tttaggtgcg ctgaatgaat ctgcgccctg aattctggta aaaaacatta tcgtaaatta
    44521 ccatttcttt caacagctta ctagtaaaca agaagttagc ctccgtgaat ataaacgtcg
    44581 ccgaattgtt aaatgggaat tacattctgt tattatttgt ggtcctcgcg cttgggctat
    44641 gtctcggaaa gttacgactt ggttcgatcc aactgggtaa ttccattggc gttttagtcg
    44701 tatcgctgtt attaggccaa caacatttca gcattaacac cgatgcgctt aatcttggct
    44761 ttatgctgtt tattttctgc gtcggggtcg aagccggacc gaactttttt tccatttttt
    44821 ttcgcgatgg gaaaaattac ctaatgttag cactggtgat ggttggcagt gcgctggtga
    44881 tcgccttagg gttaggtaag ctgtttggct gggatattgg cctgacggcc ggtatgttag
    44941 caggctctat gacgtcgaca ccggttctgg tcggtgctgg cgatacactg cgtcattccg
    45001 gcatggaaag caggcagctc tcactggcac tggataatct gagcctcggg tatgccttaa
    45061 cctatttaat cggtctggtg agtttgattg ttggtgcgcg ttacttgccg aaattgcagc
    45121 atcaggactt acagaccagc gcccagcaaa tcgcccgcga acgtggcctg gacactgatg
    45181 ccaaccgtaa ggtttattta ccggtgatcc gcgcctatcg cgtcggcccg gagctggtgg
    45241 cctggaccga cggcaaaaat ctgcgtgaac tgggtattta tcgacaaacc ggctgctaca
    45301 ttgaacgtat tcgacgtaac gggattctgg caaatccaga cggtgatgcc gtgctacaaa
    45361 tgggcgatga aatagcgttg gtaggctatc ccgacgccca tgcccgactc gatcccagct
    45421 tccgtaacgg taaagaagtt ttcgatcgtg accttctcga catgcgtatc gtcactgaag
    45481 aagtggtcgt taaaaaccat aacgctgtag gtaaacgtct cgcacaactg aagttgaccg
    45541 atcacggttg cttccttaac cgcgtcattc gtagccagat tgagatgccg atagatgaca
    45601 acgtcgtgct taacaaaggt gacgttttac aagtcagcgg tgatgcccgt cgcgtaaaaa
    45661 ccattgccga tcgcatcggc tttatctcga ttcacagcca ggtcactgac ctgctggcat
    45721 tttgcgcctt ctttgttatt gggctgatga tcgggatgat caccttccag ttcagcacat
    45781 tcagtttcgg catggggaac gctgccgggt tgttattcgc cggaattatg ctgggcttta
    45841 tgcgtgctaa ccacccgacc ttcggttaca ttccgcaagg tgcattaagc atggtgaaag
    45901 agttcggctt gatggtgttt atggcaggcg ttggtctgag cgccggtagc ggtattaata
    45961 acggcctggc cgcgattggc ggtcagatgt tgattgccgg attaattgtc agtcttgtgc
    46021 ccgtggttat ctgtttcttg ttcggtgctt atgtattgcg aatgaaccgc gcactgttgt
    46081 tcggcgcaat gatgggcgca cgcacctgcg cgccggcaat ggagatcatc agtgatacag
    46141 ctcgcagtaa catccctgcg ctgggctatg cgggcaccta cgcaatcgcc aacgtcctgc
    46201 tgacgctggc agggacaatc atcgtcatgg tatggccagg attaggataa aactgaagtt
    46261 gccctgaaaa tgaaattttt ttgcacaacc gcagaacttt tccgcagggc atcagtctta
    46321 attagtgcca ctgcttttct ttgatgtccc cattttgtgg agcccatcaa ccccgccatt
    46381 tcggttcaag gttgatgggt tttttgttgt ctgaaattta tgccttttaa aatcatgatg
    46441 ttagaagcac tgttttttaa cgatggcgac aaaatggcgg cagcgtcaaa gagagagcgc
    46501 cacctgtcct gatttcattg gatgcggctg aaccggattt gactcttttg gcgttgcaat
    46561 cgaacgaaca aaagtttcat gggtaacaaa agtatggctg cagttaatgt tctggcactg
    46621 gttgtaacgc tctttggtca atgaagatac ctgaaaactg ctgcgagtat gggcggcact
    46681 tccacacagt gggcaaatca tcatttttcg agttctcccc atttttgcta aattcacaat
    46741 aatgataccg cattattcca ttttgcaaac ttaaaagttc tccattgcga agaatcattc
    46801 catttcgaaa tcatcaatcc tcacttcaag ctccagactg gtcgtaaaac cgttatccgg
    46861 gctgacagta tgcgtcagag tcgtaatggt ccattccgca tcatctatcg gctgtttaaa
    46921 gccactgacc ttcacaggca tttctgtgta gagatctgcc cgcccttccg ccagttgtag
    46981 cgagaatgat gcaacgccgc gttgcaggcg ttcccactgc attttcgctg cccgttcggc
    47041 gttgctccgg ttggcataag tgcgattaag taccagcacg ttttcatccg tacccaccag
    47101 gtaatcgccc tgcttcgctt ccggctcttt cttctgcttc ttagtcctgc gcttacgctt
    47161 cacagtggtg ctttctttct tcgcgggttc gcgggtatgc aaccagctgg caatgacgcc
    47221 cgtgtaagct ccgcgatctg ccagggtaaa gcggtgactg tcgccgtcct tgcgtgtgat
    47281 agtgatcacc ggtagtggtt taccgctggc gcttttaccc tgcccctgcc ggatgaataa
    47341 cagattgcca tttttcaccg acgcaatagc accgtactgg cgcgccagcc gcatcaaaaa
    47401 actgccgtca ctctcattag tctggtctat atgctccacg agtttatccg acaagtcttc
    47461 acccaatgcc atcttcagtt tgtgccgcgc agctatttcc ttcaccactt ccccaacggt
    47521 ggtcttgtgc cacgactttt cacggcgggt attcagcgtt tcacgaaaat cagcacttcg
    47581 cgcccggata gtcaggcgat ccggtgcgcc agtgtgttca atctcgtcca ccgtgaatgc
    47641 ccctttcggg aaaagcggct gtcccttcca gcccagcgcc agcgtaatga cagccccacg
    47701 gcgcggcagc acgatttttc catcggcgtc gtccagctcc agatcaagct ggtccgcctc
    47761 aaagccccgg ttatccgtca gcgtcagccc catcaggcgg ttgtccagca cagtggtgat
    47821 atccctgcct tcaatactga tgctgaatgc cggagttttg ttgcctttgt taagcagttc
    47881 agagctgaaa ttcacaacag cagccctccc accgttttac tgatatcgct taaggcagat
    47941 gttgccgttc cctgcagatt attcagttgc gcactgagat caccgaacat atcggacagg
    48001 gattcatcca cgcgttttag cgacagggtg aactcaatcc ggcgcggcat accgtcgcgg
    48061 aaaaactccg ttttagtctg attcagtccc tcaatcacat acatgccgta aatcgtgccg
    48121 ctgccttcaa tcaggggcca tgcttttccc tgttctgcca tctgctccag agccagcaac
    48181 gacagcctgc cgcccgttac ctccggcata agaacaccag aaagcgtcag catgtcgttg
    48241 tccggtccca gaaactgcgt tgacgggcgt cggtttaccc ggctgtttgc cgcatgtcgc
    48301 cagctgcgtt gatactgcag ctcctgatac ggcacggtgc gcagcataaa cacgtacaat
    48361 cccagcacca tcatcatgcg tcgtatcccc cctgatcgct gtagttactc ctggcttttg
    48421 ccttcagcct gcgttcacgt tcatcaagct ggcgggccac ctcccgcgca atatcctgcg
    48481 cactttgtcc tggctgcgtt tgaatgatga tctgcatcgg tgcctcaatc cgttgaacgg
    48541 gcggcacagt ggctgcacga ctcaccatcg cttcaccgcc tttcgcggga agtgccaaag
    48601 ggtgcaacgg tggaagctct gctggcgtag cagcaacacc catcattccg gcaacaacgg
    48661 cagccagtgc agctgtattt ctccggctgg tcacatttgc cgggccgtta acaatttccg
    48721 gcccgttttc accgacgatg ccaaactgcc cgcgcgggat atacccgccg ctgtcataca
    48781 tccccgcaaa gccatatccc catgacggaa aaccacccga tggcatcatc actttaccgt
    48841 ctgcattcac cgtcgcaggt tgctgacgcg tcacgctttc cggcagtttc gcctttgcgg
    48901 cctctttact gacaatgcca agtttctcca gcaaccagga aacgccggat ttcagggagt
    48961 ccagcggatg catgaccata ttcagccctt ccgccagagc ctccccgaat cgtcgcccca
    49021 ttgccgctgc actctgcagt tcggcagagg tcgacttaac gggcgtcagc agatcagtaa
    49081 accagcccca cagcgcctgt actttgtcgc caatccactg gaacacaggc ttaagcggtt
    49141 cgaacgcggc actgacagga cctgccgccg ctttgaatcc ttccaccacg ccaccgagaa
    49201 atgtggtgat aggctgccag tatttccaga caaccagcgc cacacccgcc agtgcagtaa
    49261 ccacaagacc tatcagactg agcagagcac ctaacagacc agagacggca tacagggcaa
    49321 cgcgcagcat cgccagtgga ccggatgcga gcatacgcag caccgtgcct gtggcagcca
    49381 gtccgccgcg tagtaccgcc agtggattca taaacatcac agcaaccgca cgtaaaccgg
    49441 ataatccaga cccgcaaaag tgcaaccggc gcacctgcta cagttttcag gacatttccc
    49501 gtcagtgatg ccgtgcggcg caaagacgac aacggcgcag taagtaaacc tgcagcgttg
    49561 cccgatgaag caagcccgcg tcgcagcagt gccagtggtg cgccagccag ccaggacaac
    49621 gcgttgctgg ttcgggttac tgctgccgta acggaaggta acgttttgat acccagcaca
    49681 gagaatccca gacggatgac tgccagcggc cccagcactg cagccagcgc cacctctaag
    49741 gtgccgaggc ccacggtaac ggcagctacc acagcggcta ctttcatcag agtgcccgtc
    49801 agttccgggt tagcttccac ccagcgacgc aacgcccccg tgacgctttt caccgtgtac
    49861 agaatatcca tcagcggctg gcgcagcgtt tcccccaggc tgctgaaggt gttctgtgct
    49921 ccggttttga ccagcaacca ctgagcagaa agtgaatcct tgttgatgtc ggattctttc
    49981 tgcatggaac cgagcgcatc attgcccgca gtcagtttta actggcgctg aagttccggc
    50041 aggttgtttg ccagtttcgc tgcgtcatcg ccaaactctt taccaaacaa catggtcatg
    50101 gcagacaggc gcttatcctg tggcagcgca ttcaccttct ccagcacccg ctggatagtt
    50161 cccatcgcat ccttcgtcat ctgcttttca atcacttctg gattgagttt cagcagattc
    50221 atcccttcaa agaaactctt gctttgcatg gtggcaatgg acaattcacg caccatcgcg
    50281 tttgctgcac tggctgcaac ctcaggcgca gcgccgagtg tcaggaaggt ggaacccagc
    50341 gccgccgctt tacgataatc cagacggtca gccacaccgc ccaggcgttg catgacatca
    50401 atgatgtccg cccctttcga catggcgtta tcatccagat agttcagcgc atcaccgagc
    50461 tgctcaatat tgcgggtagg gattttgtag agctgggcga ttttccccag actttctgac
    50521 agttcatccg ctggcagttc aaaggctgtt gccgcctttg ccgccgtgct ggcgaaggcc
    50581 agcaggtcac gtttctggtc ttcccagctg tcgtcagggt ttgcgacgtt catgcgcgca
    50641 ccaccttcaa ccagtgcggc gaagtccaca gcaccgtttt ccatcggcaa ctgttcgctg
    50701 gcagccttga tggcaccctg catttcgtaa aaacgcgcag tgcggttgcc attatcgtca
    50761 cgcagaccat tgacctgctt tgccacacct ttcatggcat cttccatgct ggtatagctt
    50821 tttactgccg ccatcactgg tgcgcccatt gccagccctg cagccgtggt ggtggctccg
    50881 gcacctgcaa tacgatccct tacctcaaga cgcctggcgt aagccccccg ggcggcgtgc
    50941 atttttcgct gttgctcccc gacacgtcgt aacctcgctt cctgctcaga aagctgcctg
    51001 ttataacgca tcgtttcacg ggtaatgcgg gccgtcgcac tggcgccatc attagctgaa
    51061 ataccagcac gataaagttc tgcacgcaca agcgccgtct gctgctgcag ctttttctgg
    51121 cgttcttcca ggcgctgaac agccagccgt tgacggccca gagcaacaac ctgacgttgc
    51181 gaaggcggcc ccatcgctcc cagttcctga ctgagcaaat ttgcacgctg gcgggcatag
    51241 ttcagcctgt cgcctaattt ctgattttct gcctgcagct ttcggaagct gtccagactg
    51301 ctcccggcct gatcaagctg ctttattgca tcgcgggatt ttttgacagc agcagccagt
    51361 tctcttgaac tggcctgcgc ggatcgaaat gggcgggtga gtttgtcaac cgcattaaga
    51421 atcacctgca gacgcaggtt attgtcactc atggctggcc ccgcttctct gaatcgcttt
    51481 atcccgccat tccagcactt cggtcagcgg cataacgtca gtaacggatg gcggccagtg
    51541 aaagatggtg gcgatatcag ccaccagatc gtcaaccgtc aggctgtcgg taaaccggca
    51601 agcaccgact tcttcaacaa aaaagtgaca acctcaaccg acatggcagt gagatctgcc
    51661 gggtccatct ctgcaatttc ctgtgcagtc agtgccggac tggagatgcg ggggatcacg
    51721 gtcatcatcg cgttcacatc catatccata atggcctgca ggcgtgtgcc gcgcagcgca
    51781 ccggactgcg gtttacgcaa cacaatttcg gtaatttctg ttttaccgcg catgatggga
    51841 gtatccagtt gaatggtctt ttcagtctgc ttatcgctca ttttgttgtc ctgtaaatta
    51901 ggttctggcg cggcattccg cgccgttcag atacatcaga ggccgagggc gttgcggtgc
    51961 gcttccatca ggtccacacc gtccacaatt tccaccatgt tgatcaggtc cacttcatag
    52021 agcacctcac cattgatggt cagcttcgcg tagctgttgg tactggtcac tttggtagtg
    52081 ttgctttcgc ccgtcttcca ctcgccggaa tctacttctt tgtgacgtcc acgcaccaca
    52141 agctccacgg cctgcacttc cccggtatca tcacgctgaa tagagccggt aaagcgcagc
    52201 tggatgccat ccaccgtggc tttgcccatc tgcttaaaca gcagcagttc agtaccacca
    52261 atggaaaatt ctgtatccag tgcactgtca tccagtccca gatccacatc caccgctccg
    52321 ggcataccgc cgccgcgata cttctcatat ttgcgggtga atttcggcag cgtcagcgac
    52381 tcaacgatcc cctgccagtt gttcccgtcg ttaaacaggt tcaggtgttt taatttgcgt
    52441 ggtaaagcca tgttgtcccc ttacgcgctg acctggctgg agaaattcac caggtactga
    52501 tcggtgatgc gctgacgcag catcaggttt tcaagtggcg gcactggcgt gtagtcgtaa
    52561 tcgatggtga gtttcccggc tttcagcgtg tctttatcgt tcaccgactc gtccagccag
    52621 caatcaccac caatgagata gccctgactg accaggctac gcattttggc gcggatacct
    52681 tcgataatgt cgcgggccag cgacgggttc agcggtttgt ccaccgccca catgtgcgct
    52741 tctgccatcg tgtccatcag cacctgcgcc gtgcgggtgt agttttcgaa ggcaaagagc
    52801 ggatcatcgc tcagacagcg ggaaccccag aagcggaaac cgtctttgcg gataagcgtg
    52861 gtgatatcgt tctggttaag cagacctgca tcggttgccg ggttctgcag atcccagaac
    52921 acatcagcag aaattccggt gacaccgttc acgcccacgt tggacaggct tttgtgccat
    52981 ccggtctgct catcaatttt agcgcgcaga ccgagcgcac gggcggtggc ataagccgtt
    53041 gcttcggcat tcagcaccgt gtcccagcca gtaaagtcgg gccagatcag cattccttca
    53101 cgctgactga agttttcacg gtaagtgatt gcttcctgca ccgtcttgca accatacgct
    53161 gacaggtaag caaatccacg caggctttgc gccacgctca gcaactcagt agctaccgcc
    53221 ttcgtgtcgt gacctggcac gccgagaatg cgcggtttaa cgccgagctg tgactgggca
    53281 gataacaggg ctttcatacc tgttttttta ccctcggcgg tcactgcgcc aatgatattg
    53341 gttgtggttt cttcttccgt ttcaccctgc ggcacacgca caacaacggt cacgggtttt
    53401 gcctggtcag cgatggcatc cagcgaacgg gccagcgtgc cagactcacc cgctttaccg
    53461 ctggcggtca gcacatcagt gatcagcaca ggtttattaa gaggaaacat ttttgcatcg
    53521 gcatcatcgc ccgtgcagac catacccacg atggcggtgc tcaccgtggt aatggatcgg
    53581 gtgccttcgt tgacttcaac aacgcgcacc ccgtggtggt aatcctgagc catagtggcg
    53641 aacctcctga ttggattagg cttcgcccta tgttgaagtg attgtgcctg acaaacagct
    53701 aagcgcagtt gtgtcgttat tcacacaaaa tgacggtatt tgtccgctta caggaaaaat
    53761 caaaataata ctgactcagg gcgattcatt gctctcattc gccggaaatt ttctataaat
    53821 ggtagaaacg cccacatcaa aaatcagtgc aatacgctgt cttgattctc cggcctcgag
    53881 taaacgtcca atctgtgccc actgttcggt ggtcaactta ggacggagtc cacctactct
    53941 gcctttggca cgagctgcag ccagccctgc tctggtacgt tcaactatca gttcacgttc
    54001 catttcagcc agagccccca tgacgtgaaa aaagaaacgg cccattgggg tactggtatc
    54061 aatactgtca gtcaggcttc ggaaattcac accacgctgg cgcaactctt ctatcagcgt
    54121 aacaagatgc cgcatactgc gtcccagtct gtccagcttc cagacaacca gcgtgtctcc
    54181 tgccgatagt gtcctgagca gttttttaag ccccggtctg tcgggcttag tgccactgat
    54241 tttatcctca aaaatccgct cacatcccgc gcagttcagt gcattacgtt gcaaatcggt
    54301 gttctggtca tttgttgaca cgcgtacata gccaataagc atgatcaatc ccctgaataa
    54361 aaaccgggga tgatgccagt tagccattat ctctgcattt tcttaaacgt tggtttggga
    54421 gaaggctctg cattacctgt tggtgtgcct gttccgtggc cttcagccac tccgccgaca
    54481 ggctggctga aatgcaatgg tgcggctttt tctgctgaag aatacccgga actggcaaag
    54541 gcttacccga caaataaatt gcctgattta cgcggtgagt ttattcgtgg ctgggatgac
    54601 ggacgtggag tggataacgg aaggggatta ttaacgcttc aggacggtgc gattgtcagt
    54661 cataaccact attggggaat ctggacttca cgaactaacg accagactct ggaaagtttt
    54721 acaggcacca cgattttaaa acaaatcacg cccctgtctc cggccattga cttcgataat
    54781 tacccaattc ccaacccggc tattacagag ggtggtgttg ttgcggcaac gactaaacct
    54841 gcaggtgcga atgaaacacg cccacgaaat gtcgctttta actatattgt gagggctgca
//
LOCUS       DAJTNA010000004        53547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTNA010000004 DAJTNA010000000
VERSION     DAJTNA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53547
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.004"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(103..555)
                     /gene="tabA"
                     /locus_tag="OO161_000180"
     CDS             complement(103..555)
                     /gene="tabA"
                     /locus_tag="OO161_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5461386.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(700..1293)
                     /locus_tag="OO161_000181"
     CDS             complement(700..1293)
                     /locus_tag="OO161_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5461387.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            1364..2077
                     /gene="bdcA"
                     /locus_tag="OO161_000182"
     CDS             1364..2077
                     /gene="bdcA"
                     /locus_tag="OO161_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5461388.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2200..2592
                     /locus_tag="OO161_000183"
     CDS             2200..2592
                     /locus_tag="OO161_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5461389.1"
                     /translation="MTKSAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGTP
                     EPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAPP
                     YPTWTAVGVTWLAGFDFEIKVIERIPEL"
     gene            2872..3006
                     /gene="pyrL"
                     /locus_tag="OO161_000184"
     CDS             2872..3006
                     /gene="pyrL"
                     /locus_tag="OO161_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5461390.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3010..3945
                     /gene="pyrB"
                     /locus_tag="OO161_000185"
     CDS             3010..3945
                     /gene="pyrB"
                     /locus_tag="OO161_000185"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5461391.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3958..4419
                     /gene="pyrI"
                     /locus_tag="OO161_000186"
     CDS             3958..4419
                     /gene="pyrI"
                     /locus_tag="OO161_000186"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5461392.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4492..4878
                     /gene="ridA"
                     /locus_tag="OO161_000187"
     CDS             4492..4878
                     /gene="ridA"
                     /locus_tag="OO161_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5461393.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(5084..7780)
                     /gene="mgtA"
                     /locus_tag="OO161_000188"
     CDS             complement(5084..7780)
                     /gene="mgtA"
                     /locus_tag="OO161_000188"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5461394.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPACEGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7921..7974)
                     /gene="mgtL"
                     /locus_tag="OO161_000189"
     CDS             complement(7921..7974)
                     /gene="mgtL"
                     /locus_tag="OO161_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5461395.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8159..9124
                     /gene="treR"
                     /locus_tag="OO161_000190"
     CDS             8159..9124
                     /gene="treR"
                     /locus_tag="OO161_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5461396.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            9225..10646
                     /gene="treB"
                     /locus_tag="OO161_000191"
     CDS             9225..10646
                     /gene="treB"
                     /locus_tag="OO161_000191"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5461397.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYCIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10696..12351
                     /gene="treC"
                     /locus_tag="OO161_000192"
     CDS             10696..12351
                     /gene="treC"
                     /locus_tag="OO161_000192"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5461398.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12745..14883
                     /gene="nrdD"
                     /locus_tag="OO161_000193"
     CDS             12745..14883
                     /gene="nrdD"
                     /locus_tag="OO161_000193"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5461399.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKQLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14982..15446
                     /gene="nrdG"
                     /locus_tag="OO161_000194"
     CDS             14982..15446
                     /gene="nrdG"
                     /locus_tag="OO161_000194"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5461400.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15491..15877)
                     /gene="cybC"
                     /locus_tag="OO161_000195"
     CDS             complement(15491..15877)
                     /gene="cybC"
                     /locus_tag="OO161_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR5461401.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16061..17413)
                     /gene="pmbA"
                     /locus_tag="OO161_000196"
     CDS             complement(16061..17413)
                     /gene="pmbA"
                     /locus_tag="OO161_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5461402.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17507..18058
                     /locus_tag="OO161_000197"
     CDS             17507..18058
                     /locus_tag="OO161_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5461403.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            complement(18141..19514)
                     /gene="mpl"
                     /locus_tag="OO161_000198"
     CDS             complement(18141..19514)
                     /gene="mpl"
                     /locus_tag="OO161_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5461404.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTSQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19690..20688
                     /gene="fbp"
                     /locus_tag="OO161_000199"
     CDS             19690..20688
                     /gene="fbp"
                     /locus_tag="OO161_000199"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5461405.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20721..21716)
                     /gene="yjfF"
                     /locus_tag="OO161_000200"
     CDS             complement(20721..21716)
                     /gene="yjfF"
                     /locus_tag="OO161_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR5461406.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(21703..22725)
                     /locus_tag="OO161_000201"
     CDS             complement(21703..22725)
                     /locus_tag="OO161_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5461407.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAVMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCALIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22739..24241)
                     /locus_tag="OO161_000202"
                     /pseudo
     CDS             complement(22739..24241)
                     /locus_tag="OO161_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            complement(24381..25337)
                     /gene="ytfQ"
                     /locus_tag="OO161_000203"
     CDS             complement(24381..25337)
                     /gene="ytfQ"
                     /locus_tag="OO161_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR5461408.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25647..26177
                     /gene="ppa"
                     /locus_tag="OO161_000204"
     CDS             25647..26177
                     /gene="ppa"
                     /locus_tag="OO161_000204"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5461409.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26557..26898)
                     /locus_tag="OO161_000205"
     CDS             complement(26557..26898)
                     /locus_tag="OO161_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5461410.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(26901..30680)
                     /gene="tamB"
                     /locus_tag="OO161_000206"
     CDS             complement(26901..30680)
                     /gene="tamB"
                     /locus_tag="OO161_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5461411.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFFR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLEVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30677..32410)
                     /gene="tamA"
                     /locus_tag="OO161_000207"
     CDS             complement(30677..32410)
                     /gene="tamA"
                     /locus_tag="OO161_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5461412.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32616..33254
                     /gene="msrA"
                     /locus_tag="OO161_000208"
     CDS             32616..33254
                     /gene="msrA"
                     /locus_tag="OO161_000208"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5461413.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHLMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33577..34920
                     /locus_tag="OO161_000209"
     CDS             33577..34920
                     /locus_tag="OO161_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5461414.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34982..35188)
                     /locus_tag="OO161_000210"
     CDS             complement(34982..35188)
                     /locus_tag="OO161_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5461415.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35513..36067
                     /locus_tag="OO161_000211"
     CDS             35513..36067
                     /locus_tag="OO161_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5461416.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGTWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(36157..37068)
                     /locus_tag="OO161_000212"
     CDS             complement(36157..37068)
                     /locus_tag="OO161_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461417.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDKFAIFIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKEQLALPV"
     gene            complement(37280..38020)
                     /gene="cysQ"
                     /locus_tag="OO161_000213"
     CDS             complement(37280..38020)
                     /gene="cysQ"
                     /locus_tag="OO161_000213"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5461418.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWGVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPRFRVSIY"
     gene            38210..40153
                     /gene="cpdB"
                     /locus_tag="OO161_000214"
     CDS             38210..40153
                     /gene="cpdB"
                     /locus_tag="OO161_000214"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5461419.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(40282..40662)
                     /locus_tag="OO161_000215"
     CDS             complement(40282..40662)
                     /locus_tag="OO161_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5461420.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40751..41611
                     /gene="qorB"
                     /locus_tag="OO161_000216"
     CDS             40751..41611
                     /gene="qorB"
                     /locus_tag="OO161_000216"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5461421.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVRFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41719..42684
                     /locus_tag="OO161_000217"
     CDS             41719..42684
                     /locus_tag="OO161_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5461422.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            42792..43454
                     /gene="ytfE"
                     /locus_tag="OO161_000218"
     CDS             42792..43454
                     /gene="ytfE"
                     /locus_tag="OO161_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5461423.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43499..44911)
                     /gene="cycA"
                     /locus_tag="OO161_000219"
                     /gene_synonym="dagA"
     CDS             complement(43499..44911)
                     /gene="cycA"
                     /locus_tag="OO161_000219"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5461424.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(45220..45840)
                     /gene="fklB"
                     /locus_tag="OO161_000220"
     CDS             complement(45220..45840)
                     /gene="fklB"
                     /locus_tag="OO161_000220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5461425.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            46059..46697
                     /gene="ytfB"
                     /locus_tag="OO161_000221"
                     /pseudo
     CDS             46059..46697
                     /gene="ytfB"
                     /locus_tag="OO161_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
     gene            complement(46681..47328)
                     /locus_tag="OO161_000222"
     CDS             complement(46681..47328)
                     /locus_tag="OO161_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR5461426.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            47540..48331
                     /locus_tag="OO161_000223"
     CDS             47540..48331
                     /locus_tag="OO161_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5461427.1"
                     /translation="MKSTRPVAVITGAARGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLECLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            48341..49183
                     /locus_tag="OO161_000224"
     CDS             48341..49183
                     /locus_tag="OO161_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5461428.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQSLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            49193..49969
                     /locus_tag="OO161_000225"
     CDS             49193..49969
                     /locus_tag="OO161_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCR5461429.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            49979..51520
                     /locus_tag="OO161_000226"
     CDS             49979..51520
                     /locus_tag="OO161_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCR5461430.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGSGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYAAPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHRKQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGVPGVAPREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDITPDGLHIAQSGQIPKLVTKVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            51517..>53547
                     /locus_tag="OO161_000227"
     CDS             51517..>53547
                     /locus_tag="OO161_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR5461431.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVNYPLWQTDA
                     LVVGSGAARLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGLRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRSVEGDVAQLVASS
                     LNIVREVIAQALANENGMALVTVKDKIQARMSDYAGFICHADKVRHATRDALLLSEFV
                     QQHGLTINHAGEIAELFMWRHMTLTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSERAEDKNHRLTIQYSQGSFITEVKSLRMQPCINGIYFEK"
BASE COUNT        13145 a        14155 c        13418 g        12829 t
ORIGIN      
        1 aagaacgtct aagcattaca aattttactc acaaaaaaat atcaaaaaac gctcttttca
       61 caaactcgcc gtaaacctgg agttatttac ggcgaaaagt catcacgcca tcagcatctt
      121 cactactgct ttgcgaaccc gggctggcgc gcccactgcg cacagcggtt tatgcacttc
      181 ccccggataa aacacaacaa aatcaccttc attaaggata actgttttct catcaacgcc
      241 ttccggcaaa aatgcgatgt ctttatcagc taaccagtcg gtatccgggg tgcctgcagg
      301 ttgcgtgctg aaggtcatgc cttcctgacc ttttaacaca atctgaatgt cgagatagcg
      361 ggcatggtac tccgcacggc gagcttcgtg cggctcggtc atatcttccg agataagata
      421 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
      481 tttgatatgc tcaatcgcct ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      541 atttccgatg atcatctttt ccactcctta attaaaacaa cgtttcattt ccatcgttat
      601 acgaaaagat gatcaattcc gctagcctta acgcgtgaat tattgtctta tcacaactta
      661 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      721 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      781 tgccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      841 ttgtggatgc cgcctggcga tatagtcata aatggtcgtt tctgcggcgt gataatattg
      901 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      961 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
     1021 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
     1081 atattcattg agtacacggc taaataaccc agccttattg ccaaaagccg cgtagaggct
     1141 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
     1201 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
     1261 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
     1321 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
     1381 taagacagtt ctcatcctcg gtggtagccg tggaatcggt gccgctatcg tacgtcgttt
     1441 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaacg
     1501 cctggcacaa gagactggag cgacagcagt attcacagat agtgctgaca aagacgctgt
     1561 tattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1621 cgtctttggc gatgccctgg aattaaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1681 tattcatgct ccttaccatg cctctgttga agccgcccgg cagatgcccg aaggcggacg
     1741 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1801 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcggg ctggcccgtg atttcggacc
     1861 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1921 caacgggcca atgcgcgata tgtcgcatag ttttatggcc atcaaaagac atgggcaacc
     1981 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     2041 cgcgatgcat accattgatg gcgcgtttgg cgcataacag actacgctca attaagccca
     2101 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     2161 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatc cgctgttttc
     2221 cctgctggac gacattcact gtatgctgag catcgttatt ctgcggctat tcgttccggc
     2281 gatttgctgt ttgtttccgg gcaagtagga agtcgagagg acggaacacc agaacccgat
     2341 tttcagcaac aagtcagact ggcctttgat aatttgcatg cgaccctggc agctgcggga
     2401 tgcacttttg acgatatcat tgatgttacg agctttcata ccgatccaga aaaacaattt
     2461 gaagacatca tgacggtgaa aaatgaaata tttagcgcgc caccttatcc aacctggacg
     2521 gcggtgggcg ttacatggct ggcaggcttt gattttgaaa ttaaagtgat agagcgcatc
     2581 cctgagctct aagcaatagt gctagccgtt cgttttcaca ctccgcccta taagtcggat
     2641 gaatggaata aaatgcatat ctgattgcgt gaaagtgaaa aagaaaaaag caggaaatgt
     2701 ctgcaattat tgataccgaa cgacagttcc ccccagaatc acagtaaata aaaatgcata
     2761 tatcttaact ttaaattcaa ataaaccgtt tgcgctgaca aaatattgca tcaattgctt
     2821 gcaccgcttc tgacgatgag tataatgccg gacaatttgc cgggaggatg tatggttcag
     2881 tgtgttcgac attttgtctt accgcgtctg aaaaaagacg ctggcctgcc gtttttcttc
     2941 ccgttgatca cccattccca gcccctcaat cgaggggctt ttttttgccc aggcgtcagg
     3001 agataaaaga tggctaatcc gctatatcag aaacatatca tttccataaa cgaccttagt
     3061 cgcgatgacc ttaatctggt gctggcaaca gcggcaaaac tgaaagcaaa cccgcaacca
     3121 gagctgttga agcacaaagt cattgccagc tgcttcttcg aagcctctac ccgtacccgc
     3181 ctctctttcg aaacttccat gcaccgcctg ggtgccagcg tggtgggctt ctccgacagc
     3241 gccaatacat cactgggtaa aaagggcgaa acgctggccg ataccatttc ggttatcagc
     3301 acttacgtcg atgcgatagt gatgcgtcat ccgcaggaag gtgcggcgcg cctggccacc
     3361 gagttttccg gcaatgtacc ggtactgaat gccggtgatg gctccaacca acatccgacg
     3421 caaaccttgc tggacttatt cactattcag gaaacccagg ggcgtctgga caatctccac
     3481 gtcgcaatgg ttggtgacct gaaatatggc cgcaccgttc actccctgac tcaggcgtta
     3541 gcgaagttcg acggcaaccg tttttacttc atcgcgccgg acgcgctggc aatgccgcaa
     3601 tacattctgg atatgctcga tgaaaaaggg atcgcatgga gtctgcacag ctctattgaa
     3661 gaagtgatgg cggaagtaga catcctgtat atgacccgcg tgcaaaaaga gcgcctggac
     3721 ccatccgaat acgccaacgt gaaagcgcag tttgttcttc gcgccagcga tctccacaac
     3781 gccaaagcca atatgaaagt actgcatccg ctgccgcgtg ttgatgaaat tgcgacggat
     3841 gttgataaaa cgccacacgc ctggtacttc cagcaggcag gcaacgggat tttcgctcgc
     3901 caggcgttac tggcactggt tctgaatcgc gatctggtac tgtaagggga aatagagatg
     3961 acacacgata ataaattgca ggttgaagct attaaacgcg gcacggtaat tgaccatatc
     4021 cccgcccaga tcggttttaa gctgttgagt ctgttcaagc tgaccgaaac ggatcagcgc
     4081 atcaccattg gtctgaacct gccttctggc gagatgggcc gcaaagatct gatcaaaatc
     4141 gaaaatacct ttttgagtga agatcaggta gatcaactgg cattgtatgc gccgcaagcc
     4201 acggttaacc gtatcgacaa ctatgaagtg gtgggtaaat cgcgccccag tctgccagag
     4261 cgcatcgaca atgtgttggt ctgcccgaac agcaactgta tcagccatgc cgaaccggtt
     4321 tcatccagct ttgccgtgcg aaaacgcgcc aatgacatcg cgctcaaatg caaatactgt
     4381 gaaaaagagt tttcccataa tgtggtgctg gccaattaat tgcggttggt aataaaagtc
     4441 tggctcccta taatgagcca gactttttac cgctgtaata aaggagaaat catgagcaaa
     4501 actatcgcga cggaaaatgc accagcagct ataggtcctt acgtacaggg cgttgatctg
     4561 ggaaatatga tcatcacctc cggtcagatc ccggtaaatc cgaaaacggg cgaagtaccg
     4621 gcagacgtcg ctgcacaggc acgtcagtcg ctggataacg taaaagcgat cgtcgaagcc
     4681 gctggtctga aagtgggcga catcgttaaa actaccgtgt ttgtaaaaga tctgaacgac
     4741 ttcgcaaccg taaacgccac ttacgaagcc ttcttcaccg aacacaacgc caccttcccg
     4801 gcacgttctt gcgttgaagt tgcccgtctg ccgaaagacg taaagattga gatcgaagcg
     4861 atcgctattc gtcgctaatc ttgatggaaa tccgggctat catgcccgga ttaagtctga
     4921 tgacaaacgc aaaatcgcct gatgcgctac gcttatcagg cctacgtgat tcctgcaatt
     4981 gattgaattt gtaggccgga ttaggcgttt acgccgcatc cggcatgaac aaaactcact
     5041 ttgtctgcaa tctgaatccg ggccaccatg cccagtttat tctttactgc cagccgtaac
     5101 gacggctata aaatcctttc accaactggg ttaacgtcat ataccctgcc agaatcgcaa
     5161 ccagccacgg gaaatagctt aacggcaacg cctgtaattg cagataactg gccagcggtg
     5221 aaaacggcaa tgcgatcccg acaatcatca cgatcacggt catgatcatt aacggccacg
     5281 atgcacagct ctgaataaac ggcacacggc gggtgcggat catatgcaca atcagcgttt
     5341 gcgacagtaa gcccaccaca aaccatcccg actggaacag cgtttgcgtt tccggcgtgt
     5401 tggcatggaa tacccaccac atcaggcaaa acgtcagaat atcgaagatc gagctgatcg
     5461 gtccgaagaa gatcataaag cgccccagat ccgccggatt ccaacgctgc ggcttttgaa
     5521 tttgctcgtc gtcgacgtta tcaaacggga tcgccacctg tgacacatcg tacagcaggt
     5581 tctgaataag caagtgtaac ggcaacatcg gcaggaaggg caagaaagca ctcgccacca
     5641 gcacgctgaa cacattacca aagttagaac tcgccgtcat tttgatgtat ttcagcatgt
     5701 tggcgaaagt gcgacgtccc tcaataaccc cctcttccag caccatcagg cttttttcca
     5761 gcaggatgat atcagccgct tcacgggcaa tatctaccgc gccgtccaca gaaatgccga
     5821 tatccgccgc gcgtaaggcg ggcgcatcat taataccatc gcccataaag ccaaccacat
     5881 gcccttcgcg cttcagtaag gtcacaatgc gttctttatg catcggcgtc aggcgggcaa
     5941 acagcgtggt gcgctgtgca agatttgcca gttcgtcgtc agatagcgtt tcaatatcac
     6001 taccaatgac cacctctccc gcgtccagcc ccacttcatg gcacactttc gctgcaacta
     6061 actcactgtc gccagtaagg atttttacgg taattccact cgcttttaat gccttcagtg
     6121 ccggagcggt tgtctctttt ggcggatcaa gaaaagcaat atatccttcg aggatcaggt
     6181 cggattcatc cgcccgctgg taatctcctt cacacgctgg caggtatttc gtcgccaccg
     6241 caaccacgcg cagcccctga cgattcagcg tatcagtaac ccgcttaatc ttacgcagca
     6301 tgatgtcatc gagcggcaca atctcgccat tgtgacgcac ctgcgaacac acattgagga
     6361 tttcctgcaa tgcacctttg caaaccagct gatggtgctc ggtattttct gccactacca
     6421 ccgacatccg gcgacgctcg aaatcaaacg gaatctcatc gattttttgc caacgactgg
     6481 ccagcgagcg cgctgactct tcatccgtac cttcgagcac cgctgtatcg agcaggtttt
     6541 taagtccggt ctgataatga ctgttcaacc acgcgctatg cagcacgcgt tcgctggttt
     6601 taccggagat atcggtatga ttctccagca caattttatc ctgcgtcagg gtgccggttt
     6661 tatcagtgca cagaatatcc attgcgccaa agttctgaat agcatccaga tgtttgacga
     6721 tcactttctg tttcgacagc tttactgcgc cgcgcgccag agtcgaggta acaatcatcg
     6781 gcaacatttc cggcgttagg cctaccgcga ccgaaagcgc aaacagcgcc gcttcccacc
     6841 agtcgccttt ggtgtagcca ttgattaaca gcaccaccgg tgccatcacc agcataaagc
     6901 gaatcagcag catactgacg cggctgatcc cttgctgaaa ggcattcggc tcgctttcct
     6961 gctcactaac acgccccgcc agttgaccaa accaggtatt ggcacctgta gcaatcacca
     7021 ttgcttgtgc cgtgccgctc accacggtgg tgcccataaa acacagcgtg tcgcactcca
     7081 gcggattgct gtgctccggc tggcgagtgg tagcggcttt ttccacgggc agagactcac
     7141 cggttaacga cgcctgagcg acgaacagat cccgcgcttg caagatacgt aaatctgccg
     7201 ggatcatatc ccccgccgcc agttttataa tatcgccggg caccagctgg tcgatcggga
     7261 tctccagcca gccattttcg cctttgtcgt taattacgcg cagcaccgtc gcggtattgc
     7321 tgaccatcgc tttcagggca tctgccgctt tagtggaacg tgcttcctga ataaagttca
     7381 gcaacgtaga aatagcgacc attagcgcga taacgcccgc agcaaataaa tcttccgtgg
     7441 cgtaagaaat agcgccgaga atggtgagta aaatattaaa ggggttgcga tagcagaccc
     7501 ataaatgtac ccaccacggc gacagttgtt gtgcgggtaa tttattttca ccatgttgtt
     7561 cgcgggcaga ttccacttcc gcctgattta acccttccgg atgggtgtcg aacgttttcc
     7621 acaattcttc ttcgggcatc accgccattt tcaggcaatg cgcacttaag gacggcggga
     7681 ccaccgtatt cactgtctgc tgcgcgccag gcaatggatc acgatgaacc agacgggaag
     7741 gtaaatggcg aatgagccgg gtaaaaattt ctttaaacat aaggagtccc tccgcactgt
     7801 ctgaaacagt acagaaatgt cttcaggcgt ggcgaagcct tacctgaagg taataaagct
     7861 gagcagggac gttaaaacgt cacggcctta cgcatccggt aacgccgtga aaagacgggc
     7921 ttaccggaaa agttttaatc tccgtcgagg gagaggcgtg ggatcaggtt ccatataacc
     7981 tccggtaagt gaaaaaagcg ccgcaaataa taaggagaat ccaataatat tcgtagcatc
     8041 tatggcgata taatacctgc tggcaactaa accaacgata aaccagactt tacccattgc
     8101 tgaatgcacg ggtaaagtta ggctcaaata attaaacaac acgttacagg acaacaggat
     8161 gcaaaatcgg ctgaccatca aagacatcgc gcgcttaagc ggcgtgggga aatctacagt
     8221 ttcccgggtg ctgaataacg aaagcggcgt gagccagcgc acccgcgagc gtgttgaagc
     8281 agtgatgaat cagcatggat tttccccttc ccgctctgcg cgcgctatgc gtgggcaaag
     8341 cgataaagtg gtcgccatca ttgttacccg cctggattcg ttgtcagaaa atctcgccgt
     8401 tcaaaccatg ctgccagagt tctatgaaca aggttacgac ccaatcatga tggaaagtca
     8461 gttttccccg caattagttg ccgaacattt gggggtgctg aaacggcgta atatcgacgg
     8521 cgtagtgctg ttcggtttta ccggcataac agaagaaatg ttagcccact ggcagtcatc
     8581 gctggttctg ctggcgcgtg acgcaaaagg ctttgcttcg gtctgttatg acgacgaagg
     8641 ggcaatcaag atcctgatgc aacggctgta tgaccagggg catcgtaata tcagttatct
     8701 cggcgtgccg cacagtgacg tgacgaccgg taagcgacgt cacgaagcct acctggcgtt
     8761 ctgcaaagcg cataaactgc atcccgttgc cgctctgcca ggccttgcta tgaagcaagg
     8821 ctatgagaac gtagcaaaag tgattacgcc tgaaactacc gccttactgt gcgcaaccga
     8881 cacgctggca cttggcgcaa gtaaatacct gcaagagcaa cgcatcgaca ccttgcaact
     8941 ggcgagcgtc ggtaatacgc cgttaatgaa attcctccat ccggagatcg tcaccgtaga
     9001 tcccggttac gccgaagctg gacgccaggc ggcttgccag ttgatcgcac aggtaaccgg
     9061 gcgcagcgaa ccgcaacaaa tcatcatccc cgccaccctg tccagatcgt ttcctgaacg
     9121 ataaattgtg atcttcgctg cgtttcggga acgttcccgt ttttaaattt ttccgcgcaa
     9181 tatattctgc agccaaccaa aaatgtcatc tgccatgggg ctttatgatg agcaaaataa
     9241 accaaacgga tatcgatcgg ttgattgaac tggtcggcgg gcgcggcaat attgcgacgg
     9301 tgagccactg tattactcgc ctacgctttg tcctcaacca accggccaat gccagaccga
     9361 aagaaattga gcaactcccc atggtgaaag gctgtttcac caatgccggg caatttcagg
     9421 tggtgattgg caccaacgtg ggtgactact atcaagcact gatagcgtca accggacagg
     9481 cgcaggttga taaagagcag gtaaaaaaag ccgcccggca gaatatgaaa tggcatgagc
     9541 agttgatctc tcatttcgca gagaccttct tcccgttgct gcccgcgttg attagcggcg
     9601 gtttgatcct cggttttcgc aatgtgatcg gcgatttgcc catgagcaac ggtcaaacgc
     9661 tggcgcaaat gtacccttcc ctgcaaacga tctacgattt tctgtggttg atcggtgaag
     9721 cgatcttctt ctacctgccg gtcgggattt gctggtcagc ggtgaaaaaa atgggcggca
     9781 caccgatcct tggtatcgtg cttggcgtga cactggtttc cccccaactg atgaacgctt
     9841 atctgctcgg gcagcagctg ccggaagtgt gggactttgg catgttcagc atcgccaaag
     9901 tgggctatca ggcgcaggtg atcccggcac tgttagccgg gctggcgctg ggcgttattg
     9961 aaactcgcct taaacgcatc gtgccagatt acctctatct ggtggtggtg cccgtctgtt
    10021 cgctgatcct cgcggtgttc ctcgcccatg cgctgattgg tccgtttggt cgcatgattg
    10081 gcgatggcgt tgcctttgcg gtacgtcacc tgatgaccgg cagctttgct ccgattggcg
    10141 cggcattgtt tggcttcctg tacgccccgc tggtgatcac cggggtacac cagaccacgc
    10201 ttgctattga tttgcagatg attcaaagca tgggtggtac gccagtgtgg ccactgattg
    10261 cgctgtcgaa tatcgctcag ggctccgccg tgatcggcat tatcatttcc agccgcaagc
    10321 acaatgaacg cgagatctcc gtgcctgcgg cgatctccgc ctggcttggg gtcactgagc
    10381 ctgcaatgta ctgcatcaac ctgaaatatc gcttcccgat gctgtgcgcg atgattggtt
    10441 ctggtctggc tggattgcta tgcggcctga acggcgttat ggcgaatggc atcggcgtag
    10501 gcggcctgcc gggaattctc tcgattcaac cgaactactg gcaggtgttt gcgctggcaa
    10561 tggctatcgc catcatcatc ccgattgtac tcacctcgtt tatctatcag cggaaatacc
    10621 gcctgggcac gctggacatt gtttaatttt cttcggggcg caattgcgct ccctcgcatt
    10681 tgcaggaata acgtaatgac taatcctccc cactggtggc aaaacggcgt tatctaccag
    10741 atttatccaa agagttttca ggacaccacg ggtagcggta ccggcgattt acgtggcgtt
    10801 atccaacgcc tggactatct gcataaactg ggcgttgatg ccatctggct aacccccttt
    10861 tatgtctctc cccaggtcga taacggttac gacgtagcga actatacggc gattgatccc
    10921 acctacggca cgctggacga ttttgacgaa ctggtgacgc aggcaaaatc gcgcgggatt
    10981 cgtatcattc tcgatatggt gtttaaccat acctctaccc aacatgcctg gtttcgcgag
    11041 gcgctgaaca aagaaagccc ttaccgccag ttttatatct ggcgcgatgg agaaccagaa
    11101 acgccaccga acaactggcg ttcaaaattt ggcggtagtg cgtggcgctg gcatgcggaa
    11161 agcgaacagt actatttgca tctctttgca ccagaacagg cggatctcaa ctgggagaat
    11221 ccagcggtac gcgcagtgct gaaaaaagtc tgtgagttct gggccgatcg tggggtcgac
    11281 gggttgcgcc tggatgtggt gaatctgatc tccaaagacc cgcgtttccc tgatgacctg
    11341 gatggcgacg ggcgtcgctt ctacaccgac gggccacgag cacacgagtt tttgcacgag
    11401 atgaaccgcg atgtgtttac gccacgcggg ttaatgaccg taggtgaaat gtcctccacc
    11461 agccttgagc attgccagcg atacgcggca ctgacaggca gtgaattgtc gatgaccttt
    11521 aattttcatc acctgaaggt cgattatccc ggtggtgaaa aatggacgct ggctaaacct
    11581 gactttgtgg cgttgaaaac attgttccgc cactggcaac aaggaatgca caacgtagca
    11641 tggaatgcct tgttctggtg taaccacgat cagccgcgca ttgtttctcg ctttggtgat
    11701 gaaggtgaat accgcgtgcc tgcggcaaaa atgctggcga tggtgctgca tggcatgcag
    11761 ggaacgccgt atatctacca gggcgaagag attggcatga ccaacccgca tttcacgcgc
    11821 attactgact atcgcgacgt agagagcctc aatatgtttg ccgagctgcg caacgatggt
    11881 cgtgatgccg acgagttatt ggcaatcctc gccagtaaat cccgtgacaa cagccgcacg
    11941 cccatgcaat ggagcaacgg cgataatgcc ggatttacgg ctggcgaacc gtggattggc
    12001 ctgggtgata actatcaaca aatcaacgta gaagccgcgc tggccgatga ttcctcggtg
    12061 ttttacacct accaaaagtt aatcgcactg cgtaagcagg aagccatcct gacatggggc
    12121 aattaccagg atctgctgcc aaacagccct gtattgtggt gctatcgccg tgaatggaag
    12181 gggcaaacct tgctggtcat tgccaacctt agccgtgaga tccaaccctg gcagccaggg
    12241 caaatgcgcg gcaactggca gcttgtgatg cataactacg aagaagcctc accacaaccc
    12301 tgtgccatga atttacggcc ttttgaggct gtctggtggt tacagaagta aatcttccct
    12361 aagccccggt aatgccggtc attccggggt tttgtctgtc agcatgttgt ttttgtttat
    12421 ttttcaacca gcaaattcat taaaaaattt acataacgct gtagcgcccg tcatccgtac
    12481 actctgcttt ttactttgag ctacatcaaa aaaagttcaa acatccttga tgcaaagcac
    12541 tatatataga ctttaaaatg cgccccaacc caatatgttg tattaatcga ctataattgc
    12601 tactacagct ccccacgaaa aaggtgcggc gttgtggaaa agcggatggc gattgcggaa
    12661 agcaccggaa aacgaaacga aaaaaccgga aaacgccttt cccaatttct gtggataacc
    12721 tgttcttaaa aatatggagc gatcatgaca ccgcatgtga tgaaacgaga cggctgcaaa
    12781 gtgccgttta aatcagagcg catcaaagaa gcgattctgc gtgcagctaa agcagcggaa
    12841 gtcgatgatg ccgattattg cgccactgtt gctgcagttg tcagcgagca gatgcagggc
    12901 cgcaaccagg tggatatcaa tgagatccag accgctgttg aaaaccagct gatgtcaggt
    12961 ccatacaaac aactggctcg tgcttacatc gagtaccgtc acgatcgcga cattgaacgt
    13021 gaaaaacgcg gtcgcctgaa tcaggagatc cgtggtctgg tcgagcagac caacgcctcg
    13081 ttactcaacg aaaacgccaa caaagacagc aaggtgattc caacccagcg cgacctgctg
    13141 gccgggatcg tggctaaaca ctatgcacgt cagcacctgc tgccgcgtga cgtggtgcag
    13201 gcacatgagc gtggcgatat tcactatcac gatctcgatt actcgccgtt cttcccgatg
    13261 ttcaactgca tgttgatcga cctgaaaggc atgctgaccc agggctttaa gatggggaac
    13321 gccgagattg aaccgccgaa gtcgatctct acggcaaccg ctgtaactgc gcagattatt
    13381 gctcaggtcg ccagccatat ttatggcggc accaccatta accgtatcga tgaagtgctg
    13441 gcaccgtttg tcaccgccag ctacaacaaa catcgcaaga ccgcagaaga gtggaacatc
    13501 ccggacgccg aaggctacgc taactctcgt accattaaag agtgctacga tgccttccag
    13561 tcgctggagt acgaagtaaa caccctgcac accgccaacg gtcagacgcc gtttgtaacc
    13621 tttggttttg gtctgggcac cagctgggag tcgcgcctga ttcaggaatc tatcctgcgt
    13681 aaccgtatcg caggcctcgg taaaaaccgt aaaactgcgg tgttcccgaa actggtgttt
    13741 gcgattcgcg atggcctgaa ccataaaaaa ggcgatccga actacgacat caaacagctg
    13801 gcgctggagt gcgcaagcaa gcgcatgtat ccggatatcc tgaactacga tcaggtagtg
    13861 aaagtcaccg gttcgtttaa aactccgatg ggctgccgca gcttcctcgg cgtgtgggaa
    13921 aatgaaaacg gcgagcagat ccacgatggt cgtaacaacc tcggcgtgat cagcctgaac
    13981 ctgccgcgta ttgctctgga agcaaaaggc gatgaagcca ccttctggaa gcagctggat
    14041 gaacgtctgg tgctggcacg taaggcgctg atgacccgta tcgctcgtct cgaaggcgtg
    14101 aaagcgcgcg tggccccgat cctctatatg gaaggtgctt gtggcgtgcg tctcaatgct
    14161 gacgatgatg tttctgaaat cttcaaaaac ggtcgtgcgt ctatttcgct gggttacatc
    14221 ggcatccacg aaaccattaa cgcgctgttc ggcggcgagc atgtttacga caacgagcag
    14281 cttcgcgcga aaggtatcgc cattgttgaa cgtctgcgtc aggcagtgga tcagtggaag
    14341 gaagaaacgg gttatggttt cagtctctac agcacgccga gtgaaaacct gtgcgaccgc
    14401 ttctgccgtc tcgatactgc tgagtttggc gtggtgccgg gcgtgaccga taaaggttac
    14461 tacaccaaca gtttccacct cgatgtggag aagaaggtga acccgtacga caagatcgac
    14521 tttgaagcgc cttacccgcc gctggcgaac ggtggtttca tttgctacgg cgagtatcca
    14581 aacattcagc acaacctgaa ggcgctggaa gatgtctggg attacagcta tcagcatgta
    14641 ccgtattacg gcaccaatac accgattgat gagtgctacg agtgtggctt taccggtgag
    14701 ttcgagtgca ccagcaaagg cttcacctgc ccgaaatgtg gtaaccatga cgcctcccgt
    14761 gtgtcggtaa ctcgccgcgt gtgcggatat ttaggtagcc cggatgcacg tccgtttaac
    14821 gccggcaagc aggaagaagt taagcgccgc gttaaacatt tgggaaatgg gcagataggt
    14881 tgagctgcac taagcaaatg ccggatgtga tgtgaaagcg tcgcatcccg cgttgattgc
    14941 cggatgcagt ctacacgcgg actgataagc caggcgaaat aatgaattat catcagtact
    15001 atcctgtcga catcgtcaac ggccccggca ctcgttgcac cctgtttgtc tccgggtgtg
    15061 ttcatgaatg ccccggttgc tataacaaaa gcacatggcg ggtaaattcc ggtcagccat
    15121 ttactaaagc aatggaagac cagatcatta acgatctgaa tgacactcgt atcaaacgcc
    15181 agggtatttc cctctccggc ggcgatccgc tgcatccgca aaacgtgccg gatattctga
    15241 aactggtaca acgcatccgc gccgagtgtc cgggtaaaga catctgggtg tggacaggct
    15301 ataaactcga cgaactcaac gcagcgcaaa tgcaggttgt tgatttgatt aacgtgctgg
    15361 tcgacggcaa atttgtgcag gatttaaaag acccttccct gatctggcgc ggcagcagca
    15421 atcaggtggt gcatcatttg cgatgataag ttccctgtta cctgagtgca ggcaacaggg
    15481 aaatgaggaa ttaacgatac ttctggtgat aggcgttgcg ggtcgttttc agttgctctg
    15541 cggcagcctg cgcttctttt actttacctt catttgccag cttcagcgcg tcgtcaatct
    15601 gaccgaccag aatatcgaaa ccgtgacgga agtctttcat ctccgggctg tccggtgatt
    15661 tgccttcgag cttcggcggc gttgcttttt gcgcatccag cgctgcggcg cgcatcttcg
    15721 ttaacgcgtc tttgacttgc gccgcgttat cggctttttc gaccactttt aaattgtcgt
    15781 tgagagtttc catattgtct tcaagatcag cggcaaacga cgccgaactg aataccaacg
    15841 aggagactgc aagaatagct aacaggcttt ttcgcattgc tcacttcctt ttttatttac
    15901 acttcatcat tcacactgcc tcatcgacga ctacgctcat tcgaccgggt cacgtactgc
    15961 gagtacgctc cccggccctc attcactttc cgtctcgatg cagtgcgaat gattttgtgt
    16021 aattttttag tttatagcgc gacaggtcgc gccagttttt ttactgtccg gcgattttca
    16081 tctccggcaa cagcacagaa ccacactgta tattactgcg tgtttcaata tcgttaccga
    16141 cggtgacaat attgcgccac atatctttta aattacctgc gatggtgatt tcgctaaccg
    16201 gatactgaat ttcgccgttt tctacccaga agcccgccgc accacgggaa taatcaccgg
    16261 taattgcact cacgccctgg cccatcaatt ccgtcaccac cagcccggtg cccatctctt
    16321 tgagcatctg ctcgaagctt agaccttgtc cggcaatccg ccagttgtga ataccgcccg
    16381 catgtccggt gcttttcagc cccagttttc gcgccgaata gctggtcagc agccactgag
    16441 tcaggatgcc atctttaata atatcgcgac gctcggtgcg cacaccttcg ctgtcgaatg
    16501 gcgtcgacgc cagccctttc agcagatgcg gatgctcttc aatggtcagc cagtccggca
    16561 gaatttgttt acccagcgaa tccagcagga aggtagattt acggtaaacc gatccacccg
    16621 ctatcgcccc caccagatgg ccaaaaagcc cggttgccac ttcattggca aaaatgaccg
    16681 gcgctttcat ggtggagagt ttacgcggtg acaggcgcga taaagtacgg cgagcacagt
    16741 cggccccaac ccactctggc gtttgcagat cgctcatcgc acgaccaatg gtgtaggcgt
    16801 aatcacgctc catatcgcca ttttcttcgg caattacaca gctggagagc gaatgacgcg
    16861 ttgagcagta accctgcaac atgccgtggc tgttgccaaa aactttgaca ccgtagtggc
    16921 tgttaaagct gccaccttcg gtattggtga tgcgtttgtc cgcctgcaat gccgcctgtt
    16981 ctgcgcgggc cgccagttca atggcttcat ccggggaaac ttccgcaggg tggaacaagt
    17041 cgagatctgg tgcgtcaaag gccagcagct ctttgtctgc cacgccggca cagggatctg
    17101 gcgaggtata acgggcaata tccagcgccg cctgtacagt acgggcaatg gcctgcgggc
    17161 ttaaatcggt ggatgatgcg ctacctttgc ggttctggtg ataaacagtg atccccagcg
    17221 cgccatcgct attgaattca acattctcca cttcaccata acgcgtgctt acgctaatgc
    17281 cggtggtctt gctgacggca acttccgcac cgtccgattt gcctgaggcc aactccagcg
    17341 cagtggaaac tgcttcttcc agaatcttgc gctgcgcttc aacttgagag attactttca
    17401 ttgcaagtgc cataatgtag agagtttctc tgaagtctaa cagagaaccg tttttcagtg
    17461 cgcatcttaa ctggtaacat tagcctcttt ttttaaggag cctgagatga ctaagcagcc
    17521 cgaagactgg ctcgacgacg ttcccggtga tgacatcgaa gacgaagacg atgaaattat
    17581 ctgggtcagt aaaagtgaaa ttaaacgtga tgccgaggag ctaaaacgcc ttggcgcgga
    17641 aattgttgat ctggggaaaa acgcgctgga taagatcccg ttagatgcgg atctgcgcgc
    17701 ggctattgag ctcgcccagc gtattaagat ggaaggtcgc cgccgccagt tgcagctcat
    17761 cggtaagatg ctgcgccagc gcgatgtaga accgatccgt caggcgctgg ataagctgaa
    17821 aaaccgtcac aaccagcagg tggtgctgtt ccataaactg gaaaacctgc gcgatcgtct
    17881 gatcgatcaa ggtgatgacg ccatcgctga ggtattaaat ctgtggcccg atgccgatcg
    17941 tcagcaactg cgtactctga tccgcaacgc gaagaaagag aaagaaggga ataagccgcc
    18001 gaaatccgca cgccagattt tccagtatct gcgcgaactg gcggagaatg aagagtaatt
    18061 ttcgttgttg atgtgagtat ggtttgccgg atgcggcgta aacgccttat ccggcaatgc
    18121 tatctcaggc tgaggccgaa ttactgcgca gcttccgcct tcttcgccag accatccagc
    18181 agtttctgat ggatcccacc aaaaccgccg ttgctcatca ccagaatatg gtcgccaggc
    18241 tgagaggttt tcaccaccat atctgccagc gtatccacat cgccactcca gtgtgcaggc
    18301 tgaacgcagg cttctgccac ttctgccacc tgccacggaa tatgcgccgg ttgcagcagg
    18361 aagacttcat cggcacgacc taatgaaggt gccaaatcgt ctttgcagat ccccattttc
    18421 atggtattcg agcgcggttc cagcacagca ataatgcgcg ctgtaccacc aactttgcca
    18481 cgcagcgccg ccagcgttgc cagaatcgcc gtcgggtgat gggcaaaatc gtcatatacc
    18541 gtgacgccat tcgcttcacc acgcaactcc agacggcgac gagcattaat aaacgaaccc
    18601 agcgcgttag cggtatctgc cggcgctacg ccaacatggc gggccgccgc aatcgccatc
    18661 aggccattat gcatattatg ttcgcctacc agcgaccatt tcacttcgcc cactttttcg
    18721 ccatccagca agacttccca ttcggaagca tcggtggtca gctttttcgc ctgccagtgc
    18781 ccctgctcac ccaccagctc ctgctcgctc cagcagccca tcgccatggt ctgtttcagg
    18841 ttgatgtcat tttccggcca gataatacgg ccctgccccg gaacgatacg caccagatgg
    18901 tggaactgtt tctggatcgc tttcaggtcg tcaaagatat cggcgtgatc gaactcaagg
    18961 ttgttgagga tcagcgtacg cgggcagtaa tggacaaatt tagagcgttt gtcgaagaag
    19021 gcgcagtcgt actcatccgc ttcgataaca aagaagtcgc tttcgcccag acgcgccgaa
    19081 acctcaaagt tccccggcac gccgccgatc acaaatcccg gtttgtaacc acactgttcc
    19141 agaatccagg tcgccattcc cgcggtggtg gttttgccgt gtgtaccggc aacggccagc
    19201 acccagcggt cgcgcagcac aaaatcgtgc agccactgtg gacctgacat ataagggatg
    19261 tttttttcca gtaccgcttc cacacacgga tttccacggg tcatggcgtt gccaataatc
    19321 accagatccg gctgcggatc gagctggctg gcatcgtaac cctgaatcag ttcaatgcct
    19381 tgcttctcaa gtaaggtgct catcggcgga tacacattgg cgtccgaacc cgttacttca
    19441 tggcctaact ggcgcgccag catcgccaga ccgcccataa acgtgccaca aattcctaaa
    19501 atatgaatgc gcatacgtca ctatcctttt ttaatctgcc gctcatttta cgcatatgtt
    19561 gggcaagtga gaaacgcatt tcaggataat ccgtaatttg ctggcgcgat tcacctgagc
    19621 gcaacattga gattatttgt taagattgtt gcggtcgctt tactccataa acattgcagg
    19681 gaaagtttta tgaaaacgtt aggtgaattt attgtcgaaa agcagcacga gttttctcat
    19741 gctaccggtg agctcactgc tttgctgtcg gcaataaaac tgggcgccaa gattatccat
    19801 cgcgatatca acaaagcagg actggttgat atcctgggtg ccagcggtgc tgagaacgtg
    19861 cagggcgagg ttcagcaaaa actcgacttg ttcgctaatg aaaaactgaa agccgcactg
    19921 aaagcacgcg atatcgttgc aggcattgcc tctgaagaag aagatgagat tgttgtcttt
    19981 gaaggctgtg aacatgcaaa atacgtggtg ctgatggacc cactggatgg ctcgtccaac
    20041 atcgatgtta acgtctctgt cggtaccatt ttctccatct accgccgagt tacgcccgtc
    20101 ggcacgccgg taacggaaga agatttcctc cagcctggta acaaacaggt tgcggcaggt
    20161 tacgtggtat acggctcctc taccatgctg gtttacacca ccggatgcgg tgttcacgcc
    20221 tttacttacg atccttcgct gggcgttttc tgcctgtgcc aggaacggat gcgcttcccg
    20281 gagaaaggca aaacctactc catcaacgaa ggaaactaca ttaagtttcc gaacggggtg
    20341 aagaagtaca ttaaattctg ccaggaagaa gataaatcca ccaaccgccc gtatacctca
    20401 cgttatatcg gttcactggt tgcggatttc caccgtaacc tgctgaaagg cggtatttat
    20461 ctctacccaa gcaccgccag ccacccggac ggcaaactgc gtttgcttta tgagtgcaac
    20521 ccgatggcat tcctggcgga acaagcgggc ggtaaagcga gcgatggcaa agagcgtatt
    20581 ctggatatca tcccggaaac tctgcaccag cgccgttcgt tctttgtcgg caacgaccat
    20641 atggttgaag atgtcgaacg ctttatccgt gagttcccgg acgcgtaatc tcctgcgcct
    20701 ctccacacgg agaggcattt ttatcgctgc gcaatgttga ctcttgtcac tggcgaactc
    20761 tgacgattct cccacagcac cgttaaccca cgctgtaatg ctataaaaat aaacaacaaa
    20821 ataccgatgg cgattttcgt ccaccaggaa ctcagcgtgc cgtcaaagtt gatgtaagtc
    20881 tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa gcaccgtccc gacgccacca
    20941 cttaacaacg taccgccaat caccaccgac gcgatggcgt ccagttccac acctacgccc
    21001 gccagtgcat atccggcctg ggtataaatc gagaagacaa tccccgccag tgttgccagt
    21061 ccggtggaga gcatataaat gcgaatagtg gtgctgcgag tagaaatccc catcaggttc
    21121 gccgacgtcg cgttgccgcc aatagcgtac acctgattgc caaaacgggt acgatgcgca
    21181 aggaaaatac cgataaccac caccgccagc atcaacagcc ccatcgcact aaggcgaccg
    21241 ccgccgggga ttttccacgc aaggcttgag agcgtgtcat aaatgggatg gtttatcgga
    21301 atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa agaacatccc cgcaagcgta
    21361 atgataaatg ccgggatctt cagggcgtcg atcagaagcc ccataaatgc gccgaaggca
    21421 cagcccatca ccagcactag cggaaacgcc agcagcggcg agaggccgaa atcgccaacc
    21481 acttttgcca gaaacacgcc agtaaaggcg atcaccgaac cgacggagag atcgataccg
    21541 ccagagagga tcacaaaggt cataccaacg gcaataatcc ccagaaaagc gttatcggtc
    21601 aggatattgc agatcactct tgtggaagca aaaccgggaa actgcgtcag gcagtagaaa
    21661 tagcccagca caaagacgcc gatggtgatc atcagtggca aattacgttt tatcacggct
    21721 acgtactcct ttaatcagac tgataaagcg ttgcgactgg acaatcagcg cgcaaagcac
    21781 caccaccgct ttcaccacct ggttcatctc cggtggaaag cccgaaagca aaattccggt
    21841 gttcatcccc tgaataatca gcgcccccac taccgagagc agcaggttaa agcgcccgcc
    21901 catcagcgat ccgccgccaa tcaccaccgc gagaatggcg tccagctcca gccataatcc
    21961 ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg ataatgcccg caatcgccgc
    22021 acacagcccg ctcaacacat aagtgagcat gacgatgatc cgcgtgttta ccccggcatt
    22081 ttttgccgcc cgaatgttga taccaacggc ttcgataaac atccccagtg ccgttttgcg
    22141 ggtcaacagc cagaacagga gaagcgtcag taccgcaata atgactggcg ttggcaggaa
    22201 caacagcgat ccactgccaa accatgagag atccggcgag ttaaacgtga cgatctgtcc
    22261 ggaggtgatc agttgcgcca cgccgcgccc ggcgaccatc aggatcaggg tggcgacaaa
    22321 tggctgaatt ttgaggatcg ctaccagtat gccgttccac aatcccgcca ggatgccggt
    22381 gcccagggcg cttaacaaaa caatcggcag gctgacttcc gcgaccgtca tcacagccgt
    22441 tgtggctccg gcgatagcca tcaccgcccc tacggagaga tcaatcccac cggtggcgat
    22501 caccagcgtc ataccaatcg ccagtaacgc aacgggggct gcacggttaa gaatgtctat
    22561 ggggctaccg aacaaacgcc catcctgaag cactacctgc cagaaatgcg gggctaccag
    22621 gctgtcgacc agcagcacca gcaatagtgc taccagctgc ggcatccccg tcggccagcg
    22681 aaagcgcctt tttggcgtag tggtgtccgg aagagattga ggcatcacac tgttctcctt
    22741 acgccgcaat ggcgttcatg atagccggaa cggaaagctc tgccagccgg atctccgcca
    22801 cctgtttgcg atcgagcatg atgatcaccc ggtcggcata gcccaccagt tcttccagtt
    22861 cggaggagat caccagcagc gccagaccat cggcgcatag cgtttcaatc aggcggatga
    22921 tctcggcgtg ggcaccaaca tcaatgccac gggttggctc atcgaggatc agaaattgcg
    22981 gtcgggtcag tagccaacgt gaaagcaaca ctttttgctg attgccgccg gagagaaatt
    23041 caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg gcggataaag cgttcggcaa
    23101 tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc gcgctgggcc tggagagcga
    23161 ggatgatatt ttcccgcacc gaggcggcag cgatgatgcc atcggttttc ctgtcttccg
    23221 ggcagaagcc gatgcccagt accgaagcct gatgtggcga tcgcaggttt tgcggtttgc
    23281 ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc gaagatcact tcggcggttt
    23341 cggtacgtcc ggatcccagc aagcctgcca ggccgacgat ctcgccgggg cgcacttcga
    23401 gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt gaacgcggca acgggtttgt
    23461 cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt atccagctcg cgccccagca
    23521 tcatttttac cagttcgatc tgcggtagct cgcgcgtttc ccgacagcct acgaaactgc
    23581 cgttgcgtaa aacggtgatc cgatcgctga cctgatagat ctgatcgaga aagtgagtga
    23641 caaagatcag gctgacgccg cgatcgcgca actgacgcat caggccaaat aatagctcca
    23701 cttcctaggt gtcgaggctg gcggtgggtt catcgaggat cagcactttg gcggagagat
    23761 cgatagcccg gcaaatggcg acgatttgct gcatcgcgac tgaaaagcgg ttgagcggtt
    23821 cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc ggtggcgcgc ttttccatct
    23881 ctttgcggcg tagaaggccg aagcgtttgg gttcgcggcc tataaataga ttatcagcga
    23941 ccgacatatt gggtagcagg ttgacttcct gatagacggt gccaatgccg agttgttgcg
    24001 cgtgggcggt atttttcggt gagataacct ggccttccag ccagatggtg ccgcgatcgg
    24061 cgtggtatac accagttaat gctttgatta gcgttgattt tcccgccccg ttttcaccga
    24121 gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac gttgtctaac gctttgacgc
    24181 cggggaaaaa tttacttaat ccttcggtgc ggaggatttc ctggtgttgg tcggtcgtca
    24241 tggtttcccc cggtgtaaag cgtgctccca gtaggcatga taagacgcgg taagcgtcgc
    24301 atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg ccttatccgg cctacgaatc
    24361 ggcatcaggc atagcaaccc tcaatacccc atatttttct tcttctctaa ctcttctttt
    24421 gcggtatcag gcaggtaaag ggtggatttg gttaacgtca gcttttcagg catggtgccg
    24481 tcttttttgt atttctccag cgcgtcaaaa gcgggtcccg ccatattcgg cgtcagttca
    24541 acactggcgt tcgcttcgcc atcaatcatc gctttgtaga tatccggtac gccatcgata
    24601 gaacctgtca gaatatcttt gcccggtttc aggcccgctt ctttaattgc ctgaattgca
    24661 ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga tgtttttgcc gttgttttcc
    24721 gctttgataa agctctccat gacttctttg cctttactgc gggtgaagtc acctgactgc
    24781 gagcggataa ttttgatatt tggcgcattc ttaatggctt cggcaaagcc tttcttacgg
    24841 tcaatggcga cgctggcccc aacggtgccc tgcagctcca ccacgttgca tggtttgcca
    24901 ttcacttctt ttaccagcca gtcaccgatc aacttacctt cgaggatgtt gtcggcagta
    24961 acggtggtca tatagagaga tttgtctttc acatcgatgg aacggtcgag caagaagacc
    25021 gggatttcgg catctttcgc ctcttttaat accggttccc aacccgtcgc aaccactgga
    25081 gcgataaaga tcgcatccac cccttgtgca acgaaggaac gtaccgcttt aatctggttt
    25141 tcctgctttt gctgaccatc ggcaattttc aacgtaattc cgcgtttttc ggcctcactt
    25201 ttcgccacat tggtttctgc ggcgcgccag ccggattccg atccgacctg cgaaaatcct
    25261 acggttaatg gagcggccaa cgccatagac gacatggctg ccgagactgc agagactaca
    25321 agtaagcgtt tccacatagg gttgtcctcg tcggggtaat tattattggt gaaaagatgt
    25381 tcgcgaaaaa actatagaca attcgttatg taacggattg cgttacatca cacttcaaaa
    25441 aagtaaataa aacgttaatc acaagtttgt aatcgctttc atctcactat gaaaaatgcg
    25501 gctacggtta tggattttcc tgctctgtat accgtcttaa aactggcgaa aaaggaaaat
    25561 gaagacgaaa acaagcaaag acattcggcg cgagttggct ataatactcg gcacttgttt
    25621 gccacatatt tttaaaggaa acagacatga gcttactcaa cgtccctgcg ggtaaagatc
    25681 tgccggaaga catctacgtt gtcattgaga tcccggctaa cgcagatccg atcaaatacg
    25741 aaatcgacaa agagagcggc gcactgttcg ttgaccgctt catgtccacc gcgatgttct
    25801 atccgtgcaa ctacggttac atcaaccaca ccctgtctct ggacggtgac ccagttgacg
    25861 tactggtccc gactccgtac ccgctgcagc ctggttctgt gatccgttgc cgtccggttg
    25921 gcgttctgaa aatgaccgac gaagccggtg aagatgcgaa actggttgcg gttccgcaca
    25981 gcaagctgag caaagaatac gatcacatta aagacgtaaa cgatctgcca gaactgctga
    26041 aagcgcaaat cgctcacttc ttcgagcact acaaagacct cgaaaaaggc aagtgggtga
    26101 aagttgaagg ttgggaaaac gcagaagccg ctaaagctga aatcgttgct tccttcgagc
    26161 gcgcaaagaa taaataagtt cttctagcgc aataaccctg aacgccgggc ttcggttagt
    26221 aagggttttt ttatgcccgc gataaataaa ctctctattc caccatcatt attctcagcg
    26281 gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct gttcatagcc
    26341 acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc ccccgtttta
    26401 cccctgcaca tttgtgaacg acagcagagt tttttatgcg attgcagata tggaaagcgg
    26461 ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga tacatcgcat
    26521 aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt ctaaccagtc
    26581 gccgctttca attagcttta atccctcgac gggtcgttga taaacgtaca tccatgcact
    26641 cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc gcaaggcatc
    26701 aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg ttccgttccc
    26761 cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga tactgaaatc
    26821 gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc gtaaactgcc
    26881 gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag tgcctggtct
    26941 acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa cgtgagtgtt
    27001 gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa tacatagccg
    27061 ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa attgcttacg
    27121 ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacctc cagaccaatc
    27181 agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg tccacgtagc
    27241 aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc cgctttcggt
    27301 tcgtccgcca gaccagtgac gcgaactccg gcgattacgt cgtcttctgt agcatccggg
    27361 ttacgaatag cttcaatatt aagatacggt tgatctggcg gaccagagaa cagtagctca
    27421 cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg gatgttgatc
    27481 tgcccgttca ggcccagccc ttgtttgtcc tgaacgacgt taagatcgcc cgtcagccgc
    27541 gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat caggttactg
    27601 ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt aagcatcacc
    27661 acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat gcgcgcccac
    27721 gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa tacaacatct
    27781 ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact gcctttcgcc
    27841 gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt caggtagatt
    27901 tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt aaagttgacc
    27961 gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc cacacccgta
    28021 acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg caagttggca
    28081 ctcaccatcc ccgctgcttt ttccccacga gtaaagatgg ggtttatcat cgcaaggttg
    28141 aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc ggtcaccagc
    28201 acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa ttcggcacgg
    28261 ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag cgccgcatcg
    28321 ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc ctgcggcagc
    28381 ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc gctggcctga
    28441 gtggtttctg gcataaacgg tttcagcatg gcgaagtcga agcggttgag attcaccacc
    28501 gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc attcgggtta
    28561 agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc cagcgcaata
    28621 tcgcgggtca gcgaccacgg gccgaccagc gtctggaagc gggtattgct aagagttccc
    28681 ttccaacgtt cttctttgcg atcaaaactt cctgccagat taagctgccc ggagacaggc
    28741 tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc attcagggtg
    28801 acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc gagtttcccg
    28861 gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgtcac ggaaagttcc
    28921 tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc caccgtgccg
    28981 cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt atccagcccc
    29041 ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc tttcacttcg
    29101 gcactgtttg gccccagttc cagatgaagc ccagggatca tccactgcat ataactgttg
    29161 cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt cagcttcagt
    29221 tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt aatcaagcca
    29281 ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt aagcgttagc
    29341 tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc agttttccct
    29401 tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc tttggcatca
    29461 agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat agagagcgtg
    29521 taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata ctgcttctca
    29581 ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagcgg caatccggct
    29641 tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag attaacgcca
    29701 atctccagct gttcgcgcag cgcgccgccc acttttagct tcactttttc acctttcaac
    29761 ggctccacgt tcagtgtgct gttgagggtg atatccaccg gccagttgtc tgacagctgc
    29821 gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc gtccagttta
    29881 gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat gtccgtgtcg
    29941 cccgtcacgc gtaactgctc gcctttaaac tcttcaatgt tcaggttaag cggcaaatgc
    30001 aagtcggtca tttccggcaa taccgggcga aaaaagagat ctttcagcgt ttcgccgagc
    30061 ggcttttcat ccggctgcgg attttcaatt ttcggttcga ccacttcttc ctgcgccact
    30121 tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt cagggtcagg
    30181 gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt ggtgtcatca
    30241 atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg tgatgggata cggcgtggag
    30301 agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg cattttttta
    30361 ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac gctactgttc
    30421 cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc cacgcctggc
    30481 tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac cttgccaata
    30541 tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc gctggtggtg
    30601 cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac gccgaggctg
    30661 atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt aacccgtgtt
    30721 cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc ggcgattccc
    30781 agcgcacgcc gaccccggta ccggttttaa agtcgctgcg gcgaatatcg cttaccgctt
    30841 cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac tggtattcca
    30901 gcgatccggt tatcaacttc gaggcccctt tcaggtcacc gttggcgtat ttcggggcga
    30961 tagatttgta tttgtagccg cgaatactgc gatcgccccc ggcgaaaaaa cgcagatccg
    31021 gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt gtaacgaaac
    31081 gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg gagaaatcga
    31141 catctgagcc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag tcgccccagg
    31201 ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga taaaacagca
    31261 tcgtggtgtt ggtaatttca ccctgagtaa agtggtcgag actccagcgc aggttaatgg
    31321 cacgctgcca gccgctggag agatcccagt agcgagaagc caccagcgta gtggagtcag
    31381 attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa tattgttcca
    31441 gtggattctt cagcagcggc attttatagc tgaagtcgag ggtctgttcc ggcgcggaaa
    31501 tactggtact ggtggtcaaa ctgtgaccgt atgagttcat ccacggcttt ttccacgtcg
    31561 ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg gtgttttctg
    31621 ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct ttatcaaatt
    31681 gtggagccac caccaccgag ttaaaccagc cggtagcaga aagtcggcgg ttcagttccg
    31741 ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt tgcaggtatt
    31801 catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt tcgccactgt
    31861 tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc tgcgctttgg
    31921 taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac tttttgaaat
    31981 tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg agcaatttca
    32041 aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg ccaattaaca
    32101 ccggcacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt ggcggtggac
    32161 ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct tcacggatgg
    32221 catcatcgac gcgtgcgcga aagcggcggt ctggcgtcac ttcatcactt tcaatcgtag
    32281 aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc tgtagacgga
    32341 cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat aactgtcgga
    32401 tatagcgcac attttctcct gaatatcctt ttttcctgcc cctggaacgc cgttaaacgg
    32461 cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat atttcttatg
    32521 ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt gttacaacct
    32581 taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat aaaaagcatc
    32641 tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta gccacgctgc
    32701 atgcggtcaa cggtcactta atgaccaatg tacctgacgg aatggagatt gccatttttg
    32761 cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc gtttacagca
    32821 ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa gtgtgctccg
    32881 gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc atcagctatg
    32941 agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg cgtcagggca
    33001 atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa caggatgccg
    33061 cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat gacgatcgtc
    33121 acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat gaccaccagc
    33181 aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc gtctgtctgc
    33241 cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc tctttgtcta
    33301 tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt caatatattg
    33361 caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt tatcagcagt
    33421 ctgaatttta accctctggc gactttacag taccttacgc tatactagcc actgaaaatg
    33481 ccggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta atcaactttc
    33541 ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat tttagtcata
    33601 ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct tgccgcctca
    33661 cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca acgcgttctg
    33721 aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct gaacgcagtg
    33781 gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca cagcctgttc
    33841 tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc tttctcgtta
    33901 gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg tatgattgcg
    33961 ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta cgtttgcacc
    34021 ccgctggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt caaactgcca
    34081 atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga agccggtgcg
    34141 ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt tgagctggaa
    34201 tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt tgatctccac
    34261 gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa attcctcgtc
    34321 tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct gctgaaccgc
    34381 gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa cacgctgatt
    34441 gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc aggtgaagac
    34501 ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct caacgacgtg
    34561 atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat tgtcgcccgt
    34621 gatgagaact catggctgat tgacggcggc accccaattg acgacgtcat gcgcgtgctg
    34681 gatattgacg agttcccgca gtcgggcaac tacgaaacca tcggcggctt tatgatgttt
    34741 atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta caaatttgaa
    34801 gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat cgacagcaag
    34861 gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag cgtcgcgtaa
    34921 cccccagaaa catcaacggc tcctgaatca ggagccgtct tattactgca tagcactttg
    34981 gttaagccat ctctgtttgc agacgcataa cctgacggtt aacttcggac atcactgaca
    35041 aatgcagctt atccttcact tttgggataa gaatcttacc cttatcaaat tcaaaagcgc
    35101 caacgtcctt gatgtataac cgtccacgga acaggatctt tacgtacttc gccacctgaa
    35161 gtggattgta ccgttggaaa attttcattg ttatctcctg ctgagtatta cgcctgtgcg
    35221 gttccacaat cggcccaact attatgagac gcaaatttta atgcttagtg actatagact
    35281 atccgggcaa tgtttccacc gcgtataact ttttttacct aaaggttaca attattcaga
    35341 attatctttt taccgaagcg cggtcttcag tataagcatt catttttcat atgatttgtg
    35401 cgcttgaccg caaactggca tcacacttgc gggaaattcg ataaatagca catatgatta
    35461 aaactcagat ccaagtggtc ggatcacctg catatcataa gaaggaaaca ccatgaccct
    35521 acgcaagatt ctggcactca cctgcctgct gttgccgatg atggcttccg cacatcagtt
    35581 cgaaaccggt cagcgagtgc cgccgattgg cattaccgat cggggcgagt tggtgcttga
    35641 taaagatcag tttagctaca aaacctggaa cagcgcgcag ttagtgggaa aagtgcgagt
    35701 actgcaacat attgctggtc gcacctctgc aaaagagaaa aacgcgacgc tgattgaagc
    35761 gattaaatca gcaaagttac cgcacgatcg ttaccagacc accaccattg ttaacaccga
    35821 cgacgcaatt ccgggttccg gcatgtttgt gcgcagcagt ctggagagta ataaaaagct
    35881 ttatccctgg tcgcagttta ttgtcgatag caatggcgtc gcgcgcggta cctggcagct
    35941 ggatgaagaa agttccgctg tagtggtgct ggataaggat ggtcgcgtgc aatgggccaa
    36001 agacggggcg ctcacacagg aagaggtgca gcaagtgatg gacctgctgc aaaagttgct
    36061 caaataatgg caataacata aattctgcaa aaataatttt cccctatcgt tatctatggg
    36121 ataggggaat taggtctctg agctcttaca ccgaactcat actggtaacg ctagttgctc
    36181 ttttttaaac gcctcaatgg ttgatttata tttctcgcaa atctcctctt cggtaatatc
    36241 actatctacg gccagattcg cacttactat cgcatccact tgttcggcta attcgacttg
    36301 ttggcaacct gcattaacca gaaaattata aataagagag taatcttcat cctccaaaac
    36361 aacagaggaa ccattacgaa aacgttcatc ccctccatag attttaagcg caaagcccaa
    36421 agattctatg aatatggcaa atttatcatc aattattttt tcgtaaatat accatccatc
    36481 gcattcttta attccacctc ctggtatttg gagttcaaat ttaccccgtt ttaatttgct
    36541 tactatatcc tccaataagg cataacctaa agacacgtca ccaatcgcct ggtgaatata
    36601 acctttaatc cttttcggta tgtcagactg ttttttctgc agaatatccc aggcatacaa
    36661 tcttctgttt tgttctaacg gaaattcacg gtttttgaaa tccatgaatg agttatactc
    36721 atttattgtt atggggattt cttcgtattc aaatccactg cctcgcaatg tcattgtata
    36781 ttcggcaccg ttatctttaa ggctgaaact gataagaaac ttatccatgg ctttaacagt
    36841 ttcatttttt gatgaaagca cttgataact atctttatca atctgagttg cgcaacgaaa
    36901 aaactcccac aaagttaaca ggctatcata attcgaaacc caatcttttt tcttaaatgc
    36961 atgctgaata ttattaaaaa aattgtttga atgtgttttt gtatgcgatg attttacgaa
    37021 taactccacc gcaccaataa aattattgtg tctgatcctg ggtaacatat ggctacctta
    37081 ttttatttgg ttattttatg tcgccattaa atatctcatt atagaagtaa actatcaaaa
    37141 aacgctcatt attaaaatgt aacttattag caattgaatt tttgcacttt aaatacaatc
    37201 tctttttatt tataactttt taggttggga gaaatcagga tggcagaatc aggaaataca
    37261 ctgtttctgc catctgaatt tagtaaatag acactctgaa cctaggattc aggaacgact
    37321 cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg aacgtgcgct ccggcagctg
    37381 cggccacagc atgtccagcg gcggtgtccc aaatattcgt tggtccgaag cgcgggtaca
    37441 gctgcgcctg tccttccgcc accaggcaga atttcagcga agagccgatg gacgtggtct
    37501 gatgttcgcc aagctgttgc agatactctt tcagctccgc atctgcatgg gaacggctga
    37561 taaccaccag cggcgggcgc gcatctcgga cctgaatctg cttgcgcaca ccgcactctt
    37621 ctttccaggc cttgccttct gccgcgctgt acattacgtt cattaccggc gcatacacca
    37681 cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt aacggtgaat tcgccattgc
    37741 gtttaataaa ctctttagta ccatccagcg ggtctaccag ccagtaacgc tgccagtgct
    37801 gacggactcc ccaaccggga ggatcttctt cagaaaggac cggaatatcc ggtgtcagcg
    37861 tacgtaaacc gtccatgata acggtgtgtg cggcaatatc cgctgccgtt accggagaat
    37921 tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc gtagacctgc ataatggcat
    37981 cgcctgcatt ccgtgcaagc tggcatactt gatctaacat ttctccacct cgtctctgtg
    38041 agcggtgtta acttattgtt ttacttatac cctatcgtta atgaatgcgc caactgtgat
    38101 agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc actgttctat aagtaagacg
    38161 tttattctcc cttttctttc gtattcccga tgataaaagg atgtccctga tgattaagtt
    38221 tagcgcaacg ctcctggcta cgctgattgc cgccagtgtg aatgcagcga cggtcgatct
    38281 gcgtatcatg gaaaccactg atctgcatag caacatgatg gatttcgatt attacaaaga
    38341 caccgccacg gaaaaattcg gactggtacg tacggcaagc ctgattaacg atgcccgcaa
    38401 tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg attcagggga gtccgctggc
    38461 cgattacatg tcggcgaaag gattaaaagc aggtgatgtt catccggttt ataaggcgct
    38521 gaatacgctg gattatacgg tcggtacact cggcaatcat gaatttaact acggtctgga
    38581 ttacctgaaa aatgcgctgg cgggagcgaa attcccttat gtaaatgcca acgtcattga
    38641 cgccagaacc aaacagccaa tgtttacacc gtatttaatt aaagataccg aagtggtcga
    38701 taaagacgga aaaaaacaga cgctgaagat tggctatatt ggcgtcgtgc cgccgcaaat
    38761 catgggctgg gataaagcta atttatccgg aaaagtgacg gtgaatgata ttaccgaaac
    38821 cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc gatgtcgttg tcgttctggc
    38881 gcattccggg ctgtctgccg atccgtataa agtaatggcg gaaaactcag tttattacct
    38941 cagtgaaatt ccgggcgtta acgccattat gtttggccat gctcacgccg ttttcccggg
    39001 taaagatttt gctgatatcg aagaggctga tatcaccaaa ggcacgctga atggtgttcc
    39061 ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg gtcgacttac aactcagtaa
    39121 tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa gcacggccga tttacgacat
    39181 cgccaataaa aaatccctcg cggcggaaga cagcaagctg gtagaaacac tcaaagccga
    39241 tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt aaatctgccg acaatatgta
    39301 tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa gtcgtgaaca acgcgcaaaa
    39361 agcgtatgtc gaacattaca ttcagggcga tccggatctg gcaaaactgc cggtgctttc
    39421 agctgccgca ccgtttaaag ttggtggtcg caaaaatgac ccggcaagct atgtggaggt
    39481 ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt tatctctacc ccaatacgct
    39541 gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg ctggagtgct ccgccggaca
    39601 gtttaaccag attgatccta acagcacgaa accacagtca ctcatcaact gggatggttt
    39661 ccgcacttat aactttgatg ttattgatgg tgtgaattat cagattgatg ttacccaacc
    39721 cgcccgctat gacggcgagt gccagatgat taatgccaat gcagagcgga ttaagaacct
    39781 gacctttaat ggcaagccga ttgatccgaa cgctatgttc ctcgttgcca ccaataacta
    39841 tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc catatcgctt ttgcttcacc
    39901 ggatgagaac cgctcggtgc tggcagcgtg gattgctgat gagtcgaaac gtgcgggaga
    39961 aattcacccg gcggcagata acaactggcg tttagcaccg atagctggcg ataagaaact
    40021 ggatatccgt ttcgaaactt ccccgtcaga taaagccgca gcttttatta aagagaaagg
    40081 gcaatatccg atgaataaag tcgcgaccga tgatatcggg tttgcgattt atcaggtgga
    40141 tttgagtaag taaaacactt ctttttcggt ctataaatta tcaaccgcat ccggcattta
    40201 ttggcggatg cgatgctggc gcatcttatc cgccctacaa gccatgcacc gtagaccaga
    40261 taagctcagc gcatccggca gttatgccgc acgttcatcc cgcaccgcca acacctcggg
    40321 caaattcaac tcaatccagt ccgccagtgc agcaaccttt tcgctcacct gctcgcccag
    40381 cggcgtgagg ctatattcca catgcggtgg caccaccgga tacgcgatac ggttaagaaa
    40441 accatcctgt tctaacgcct gtaacgactg cgcaagcatc ttttcgctca ccccgccgat
    40501 tttgcgccgc aggtcgctaa agcgatgagt accttcgcgt agcgccacca gaatcaacac
    40561 cccccaacgg ctggtgacgt gtttcaacac ctcgcgcgac gggcactgtt ccgcaaagag
    40621 attaccctct ttcagttgtt gcgacaggct aacctgactc atttcatact tacctttttg
    40681 tacgtactta ctaaaagtaa gtttaggtgt tagcgtattt aaacacaaga caaaacgatg
    40741 gagacttccc atgatcgcta ttactggtgc cactggccaa cttggtcact atattattga
    40801 atccttgatg aaaacggttc ctgccagcca aatagtggct atcgttcgta atccggcaaa
    40861 agcccaggct ctggcagcac aaggcattac cgtgcgtcag gctgactacg gcgatgaagc
    40921 cgcactgaca tctgcacttc agggagtgga aaaactactg ctgatctctt ccagcgaagt
    40981 gggtcaacgt gccccgcagc atcgtaatgt tattaatgcc gcaaaggcgg ctggcgtgag
    41041 atttatcgct tataccagcc tgctacatgc agatacctcc ccgctcggcc tcgccgatga
    41101 gcacattgag acggagaaaa tgttggctga ttctggcatc gtttacaccc tgctgcgcaa
    41161 cggctggtac agcgaaaact acctcgccag cgccccggca gcactggaac acggcgtatt
    41221 tatcggtgcg gcgggcgatg gcaaaatcgc ctcagcaacg cgggcagatt atgcggcagc
    41281 tgcggcacgc gtgattagcg aagccggtca cgaaggcaag gtttacgaac tggcgggcga
    41341 tagtgcctgg acgttgacac agttagcggc agagctgacc aaacagagcg gcaaacaggt
    41401 tacctatcaa aatctgagcg aagccgattt cgccgcggca ctgaaaagcg tcggactgcc
    41461 cgacggactg gcggatatgc tggcggattc tgacgttggc gcatcgaaag gtggtctgtt
    41521 tgataacagc aaaacgctta gcaaattgat tggccgccca acgacaacgt tagccgaaag
    41581 cgtaagccat ctttttaatg ttaataacta gttaattaaa gtggcatcct cccgcatcct
    41641 ctctaataat gacgggatgc cgggagcaat catgtctgct tcctgaactt tcttctgaca
    41701 gaccaatgga tgccagtaat gattagcggc gtgctgtacg ccctgttagc agggttgatg
    41761 tgggggctta tttttgtcgg gccgttgatc gtaccggaat acccggcgat gttgcagtcg
    41821 atggggcgtt atctggcgtt agggttaatt gcgctgccca ttgcctggct gggacgcgtg
    41881 cgtctgcgtc agttggcgcg tcgcgactgg cttaccgcct tgatgctcac tatgatgggc
    41941 aacctcatct attacttctg ccttgccagt gccattcaac gtactggtgc gcctgtttcc
    42001 acgatgatta tcggcaccct gccggtggtg attcccgttt ttgccaatct gctttatagc
    42061 cagcgcgacg gcaaactcgc gtggggaaaa ctcgccccgg cactggtttg tattggcatc
    42121 ggcctggcgt gtgtgaatat tgctgagtta aaccacggac tccccgattt tgactgggca
    42181 cgttatacct caggcatcgt gctagcgtta gtttccgtgg tctgctgggc atggtatgcc
    42241 ctgcgcaacg cccgctggct gcgggagaat cccgacaaac atccgatgat gtgggcgacg
    42301 gcgcaggcgc tggtcacact gccggtttct ctcatcggct atctcgtcgc ctgttactgg
    42361 ctgaatacgc aaacgccgga cttctcccta ccttttggcc cccgtccgct ggtgtttatt
    42421 agtctgatgg ttgcgatagc cgtgctttgc tcatgggttg gcgcactctg ctggaacgtc
    42481 gccagccagc gattaccgac agtgattctc gggccgctga tcgttttcga aacactggca
    42541 ggtttgctgt acaccttttt gatacgccag caaatgccgc cgctgatgac gctgagcggt
    42601 atcgcgctgt tagtggttgg cgtggtcatt gcagtcagag caaaaccgga aaagccttta
    42661 actgaatctg tctcagaaag ttgacatact ggctgtgagt taaataagcc tctgctacgt
    42721 aagggttata gcttttgcct taaagatgta tttaaaatgc atcttatctt attaagaatg
    42781 aggtatcagc tatggcttat cgcgaccaac ctttaggtga actggcgctc tctattcctc
    42841 gcgcttcagc tctgtttcgt aaatatgata tggattactg ctgtggcggt aagcagacgc
    42901 tggcgcgcgc ggcggcacgt aaagaactgg atgttgaggt cattgaagct gaactggcaa
    42961 agcttgctga acaaccgatt gagaaagact ggcgtagcgc cccgctggca gaaatcattg
    43021 accatatcat cgtgcgctac cacgatcgtc accgcgagca actgccggag ctgatcctgc
    43081 aagcgactaa agtcgagcgc gttcacgccg acaaaccgag cgtgccaaaa gggctgacaa
    43141 aatacctgac catgctgcat gaagagcttt ccagccacat gatgaaagaa gagcagatcc
    43201 tcttcccgat gatcaaacaa ggcatgggca gccaggcaat ggggccaatc agcgtaatgg
    43261 aaagcgagca cgatgaagcg ggcgaactgc tggaagtgat caaacacact accaataacg
    43321 tcacaccgcc gccagaagcc tgcaccacct ggaaagcgat gtataacggc attaatgaac
    43381 tgattgatga cctgatggat cacatcagtc tggaaaacaa tgtactgttc ccacgcgcgc
    43441 tggcgggtga gtgatctaaa agctggatgg cattgcgcca tccagcatga taatgcggtt
    43501 atttccgcag ttcagcagcc cgcttcttac caataaacaa ccagcccagc cccagtgcga
    43561 taaaccacag cggggtaacc agcagcgcct ggcgagtgtc atcttccagt gtcagcaaca
    43621 cgaccacgaa cacaaagaac gccatacata cccagcacat cagcttgccg agcggcatct
    43681 tgtagatcga cttctcatgc agatgaggac gctgtttgcg gtacaccagg tacgagcaaa
    43741 ggataatcgt ccagacgaac ataaacagaa tcgcggaaac ggttgtaatc atcgtgaacg
    43801 caccaatcac gctaggattc acatacagca tcaccacgcc accgagcagg cagatgcagg
    43861 agaaggtcag ccctttcgct ggtactgcgc gcttagaaag tttagcgaac gctttcggtg
    43921 ccacaccttc ctgcgccaga ccaaacagca tacggctggt agagaagacg ccgctgttag
    43981 cggaagacgc cgcagaggtc agcaccacaa agttgatcac gctggcagcc gcaggcaaac
    44041 ctaccaacac aaacagctca acgaacgggc ttttctccgg gactaccgaa ctccacggtg
    44101 tcacggacat aatcacaatc agcgcgaaga cgtagaacat aatgatacgg atcggaatgg
    44161 agttaatcgc gcgtggcagt gatttctccg gatctttggt ttccgcagct gttgtaccta
    44221 ccagctcaat ccccacgaaa gcgaaaaccg ctatctggaa tccggcaaag aagccactta
    44281 aacctttcgg gaaccagccg ccgtcattcc acaaatgcgc gaatgacgct tccacaccag
    44341 tcggtgactg aaagtgcatc gccaccatga ccaggccgac gacaatcagc gacacgatgg
    44401 cgacgatttt gatcatcgca aaccagaact ccatctcacc gaacattttc acggtggcaa
    44461 ggttgagcgt cagcagcagc actatcaccg ccagcgaggc gacccagtcg gagagatcgg
    44521 ggaaccagaa ctgagcataa gccgtgatcg ccaccacgtc tgccataccg gttacaaccc
    44581 agcagaacca gtaagtccag ccagtgaaat atcctgccca cggcccgagt aaatcggaag
    44641 cgaagtcact aaaagatttg tattccagat tcgaaagcag caattccccc attgcccgca
    44701 tcacgaaaaa gagcataaaa ccaatgatca tataaacgaa aatgatcgac ggcccggcaa
    44761 ggctaatcgt tttgccagac cccataaaca accccgtacc aatggcaccg ccaatggcaa
    44821 taagctgaat atgtcggttt gtgagattgc gccgtagcga ctgttcagcc ggagcctgat
    44881 catcggcaac gacttttacc tgatctacca tgtttttttc ttcctgtacc tgtctgtgtt
    44941 gttcaggctc tacggccttt agtcagtcta tgatacgacg aatctgtatc gggactcatc
    45001 gatattaggt aacaatacgc gggatgaata ctaagattta gaactaatgt taattttatg
    45061 tttaaagtga gtgtcatatc acggtgataa cgcgaaacag ctcacaaaaa tacacgctta
    45121 cggggtcttt gcaacacaaa atagcgccgt ttggcaaact gaagggttta ttgctgaatg
    45181 cctgctcccc tctggttcga ggggaacaga atgcgctgct tagaggattt ccagcagttc
    45241 gacttcaaac accagggtgc tgaacggagg gatggatgca cctgcgccgc gctcgccata
    45301 tgccagttcc tgcgggatag tcagttccca tttagaacct accggcatca gagtcagtgc
    45361 ttcaatccag ccagggatca cgccgttaac cgggaattca gcgggttcac cacgagcaac
    45421 ggagctgtca aacacggtgc cgtcgatcag tttaccggtg taatgaacac gaacgcggtc
    45481 ggtacgtgcc ggaattgcgc cttcaccctg gttgatcacg cggaattgca ggccagattc
    45541 ggtgctattc acaccttctt ttttggcgtt ttcttccagg tatttcacac cttcagcagc
    45601 catcgcctgg aaacgctgac gacgaacggc atcggcgcgt tcgtggattt cacgcagcgc
    45661 gcgatgcacc acatcaaccg gaacagccgg atgtttgcct tccagcgcat cggcaatacc
    45721 tgcaaccagt gcttctggca gcagcccttc caggccagat tcactcagtt gttgccctac
    45781 ctgcaaacca atgccgtagc ttgcttgcgc ttcgatggtg tcaaaagttg gggtggtcat
    45841 cgtatttcct ttcttagggt ataaaagtag cgggcagcat atcagccaca cttctttggg
    45901 taaaactttg tcgcaggaat gatgacaaat cccggggaaa aagaaacaat agcaatatcc
    45961 cgtatgaata caataaaggc gggtttacca cggacatcat agcgttagct tattcatagg
    46021 ctatgattga ggaacaagac gcggagcagg aggaaaccat gcccgggcgc tttgaactaa
    46081 aaccaaccct ggagaaagtc tggcacgcgc cggacaattt tcgcttcatg gacccgctgc
    46141 cgccgatgca tcgtcggggg atcattattg ccgccattgt attggtggtc ggatttctgc
    46201 ttccatctga tgatacgccc aacgcgccgg tggtgacgcg tgaagcgcag ttagatattc
    46261 agtcgcaatc acaaccgccg acagaagaac agctgcgggc gcaattagtc acaccacaaa
    46321 atgatcctga tcaggtggct ccggtcgctc cagaacctat acaggaaggc cagccagaag
    46381 agcagccgca aactacccaa acgcaaccat tccagccaga tagcggaatt gataatcaat
    46441 ggcgctctta ccgtgtcgaa cccggtaaaa cgatggcgca gctgttccac gaccacggac
    46501 tgcccgctac cgatgtctat gcaatggcgc aggtataagg cgcgggtaag ccgctgagta
    46561 atttgcaaaa cgggcagatg gtgaaaatcc gccagaacgc cagcggcgtg gtgacgggct
    46621 taaccattga tacggggaat aatcaacagg tcctgttcac ccgccagccg gatggcagtt
    46681 ttattcgtgc gcggtagatg aagggtcaac caaaagccag ctaaggattg tctctttgat
    46741 gaccttacgt ctggcttcca gtgcctgggg ggaaaccata ctgatatgat aaaccaggcc
    46801 aagcgtatgc tggttgatca aataatatcc gccaagcgca gcaatattaa tattcacctg
    46861 caatgggtcg atatctggct taaaaatatt gtgtttcttc ccttcatcca ataaggattc
    46921 cattaactgc aagtgcgcat ggttaatttc taagagacgc tgtgattttg cataatgcac
    46981 acctttactt tgattttcac tatgcacaat ttttaaaaac caggggttag caatataata
    47041 atcccatgta aaatcaataa ttttctcaat agctttttgt ggcggaagat ctgagaaatc
    47101 aagggccttt tctttttgtc gaatatcgtt ccaggtatat tccagtactt caataaataa
    47161 attttcttta ttaccaaagt gatgataaac cgtctgttta ctacaccccg cagccttaac
    47221 aatattatcg accctggccc cttcataacc atattcggca aattcattaa ccgcgcataa
    47281 cagcagcttt tccttcaggc tctgtacgtt gctggtatca agatacataa cgcttcccca
    47341 cgacctcttc gtggtgacta tcataacctg atttgatttc agcttcagaa aaaagggaaa
    47401 aaacttgtac tttcatcagg ttatcaaatg tcctgtgatc gtcatcgcat atcaaccaac
    47461 tagatggttg atctttttat cctccagtcg caagctaatc atcaccaagg aaacaaaatt
    47521 caaaaaagag agctattgta tgaaatccac tcgtcctgtt gccgttatta ccggagccgc
    47581 tcgcggtatt ggaaagggct gtgcgcagga gcttgctcgt ggcgggttta acctgctgat
    47641 taacgatcgt cccgatgccg acagcgtaga aaaactgcat atcacacaac aagaatgttt
    47701 ggcggaaggc gtggaagtga tttgcttccc tgctgatgtc ggtgatctat ctctgcatga
    47761 ggagatgctc gacgcggcgc aaaaccagtg ggggcggctg gagtgtctgc tcaataacgc
    47821 ggggatttca gtgaaaaaac gcggtgacct cctcgacctc gaaccagaca gcttcgatca
    47881 aaacattgcc attaacaccc gcgcgccgtt cttccttgct caggcattca gtaagcgctt
    47941 actggcacag ccgaaaccgg aagcggagct gccgcaccgc tcgattattt tcgtcagttc
    48001 catcaacgcc atcatgctgg cgatgaatcg cggcgaatac accattgcca aaaccgccgt
    48061 ttctgccgct gctcgtctgt ttgctgctcg cctttgtaat gaacaaattg gcgtttatga
    48121 agtgcgccct ggtctcatca aaactgatat gactattccg gcaacggctt attacgacga
    48181 actgattgcc aaaggtctgg tgccgtgggg acgttggggt tatccggctg atattgcttc
    48241 caccgtccgc gcgatggcgg aaggtaaact gatttacacc tgtggtcagg cggtcgccat
    48301 cgacggcggc ttaagtatgc cgcgcttctg aggctgaatc atgagagatc tgttacaacg
    48361 cccggattta ttcagtatca ataccgcgac actgggctat aaaacgccgc tacccgccat
    48421 tattgatgcc tgcgccgcac gcggtattgg cgcaattgcc ccctggcgca gggagttgca
    48481 gggtgaagat ctccagcaaa tcacccgcca gcttgccgcc agtaacatga gcgtttccgg
    48541 tttgtgccgt agcacttact acaccgcgcc gacactggcg gaacgcaaac tggcgattga
    48601 tgataaccgc cgggcgctgg acgacgcagc ggtgcttaac gccgcctgtt atatgcaggt
    48661 ggttggcggt ctgccaacgg gcactaaaga tctatatgaa gcgcgagaac aagtgaaaca
    48721 agggattcgc cagtcgctgc cacattcgaa agatgtcggc gtgccaattg cgctggagcc
    48781 actgcacccg atgacagctg ccgatcgctc ttgcctgtgc acgttacgtc aggcgctgga
    48841 ctggtgtgat gaactcgatc ccgatggtga gtttggcctc ggcgtggcgg tggatgttta
    48901 tcacgtctgg tgggatccgg atctcgccag tcagatcctg cgtgccggaa aacgcatcct
    48961 cgcgtttcat gtttccgact ggttagtgcc gaccactgat ctggtcaatg accgtggtat
    49021 gccgggagat ggcgtcatca atattccgtc aattcgtcgg ctggttgaaa acgccggatt
    49081 taatggtgct attgaactgg aaatattctc gccttactgg tggcagaaag atattaacag
    49141 cacgctggat attagcgtcg atcgcatcgc gcattattgt taaggaatga tcatgagtga
    49201 ccaacccgtt ctgttcagca gagtggcggc ttcctgccgc ctgacattga accgtgaaga
    49261 taaatgtcat gctattaacg aagagatgat cgaatctctc gaccattatt taaatgaaat
    49321 tgaaaacgac accacattac gattagtcga attaacggca accggcgata aattcttttg
    49381 tgcgggcggc gatattaaat cgtggtcggc ctattcgcca ttagatatgg gcagaaaatg
    49441 gattaaacgc ggtaatgacg tttttaatcg cctgcgaaac ataccccaat taaccgttgc
    49501 gaatctcaac ggtcatacca tcggcggtgg tattgaactg gccttgtgct gcgatattcg
    49561 tattgcccgc cccggcgcga aattctctaa cccggaagtg atgctcggca tggtgcctgg
    49621 ctggatgggc attgaacgtg tgcttaatca ggtcggtccg gtggtcggtc gccagatgct
    49681 gatgctgggt aaacgcctga cggcgcagga agctcaggcg gcgaatttaa ttgatgaagt
    49741 ggtagaaaaa gaacaggtgg aaagctggat ggcgaaccag ttagcgcagc tggaaaaatg
    49801 cggtccggtg gcactggcgc atattaagca actgattctg gcactggaaa ataaacatgc
    49861 cgattacccg caccagctac tggccggatt aatgtccgcc actcaggatt gccagcaggc
    49921 gacgcgcgcc tttgccgaaa aaagtagcgt gagcttccat aatcaataag gaaattgtat
    49981 gcgtaagata acaaccgccg aggcactggc ggcgcaaatt caggacggtg cgactattgc
    50041 tattagcggt aacggcggcg gtatggtaga agccgaccat attctggctg ctattgaagc
    50101 gcgtttcctg caaaccggac accctcgcga cctgacgcta atccactcgc tgggtattgg
    50161 cgatcgcgac tgcaaaggca ctaaccgttt tgcgcacgca gagatgctca aacgcattat
    50221 tgccgggcac tttacctggt cgcccaaaat gcaggcgctg gtaaaaaata acacgattga
    50281 agcctactgc tttcctggtg gtgtgattca agcactgcta cgggagatcg gctccggacg
    50341 tccggggctt tttacccacg ttgggctggg atcgtttgtt gatccacgca atggcggcgg
    50401 taagtcgaat gaatgcacta ccgacgacct ggtagaactg attgaaatcg acggtgaaac
    50461 caaacttcgt tatcgccctt tcaaagtgga ttacgcgatt ttgcgtggca cttatgccgc
    50521 tcctcgaggc aacgtcagcc ttgaagaaga agcgattgat atggatagct attccatggc
    50581 gctggcagca cacaacagcg gcggcaaagt gttcgtacag gtacgcgatg tgctggaagc
    50641 tggcgcgatt gaaccacgcc gagtcaaatt accgggcatt ctggttgatg gcatcgttga
    50701 acaccgcaaa cagccacaaa cctatcttgg cggttacgac ctgaccatca gcggccaaca
    50761 tcgccgtctg agttctaacg acgctattga actggttagt catccggtac gtcgcctgat
    50821 tgcccgtcgg gcagcacgcg aactggtagc tggcgcttca accaactttg gctttggtat
    50881 tcccggcggc gttccaggag tagcgccgcg cgaaggcgtt ccttaccaaa gtttatggct
    50941 gagcgtcgag caaggcgtac ataacggcat gatgctggat gatgcgtttt tcggctgcgc
    51001 ccgcaacgcc gatgccatta ttccatcact cgatcaattt gagttctaca gtggcggagg
    51061 gatcgatatc accttcctcg gcatgggaga gatggatcag tacggtaacg tcaacgtctc
    51121 ccatctcaat ggcaatctga ttggccccgg cggatttctc gaaattgcgc aaaatgcccg
    51181 taaagtggtg ttctgcggca cgttcgacgc taaaggcagc aagatcgata taacaccaga
    51241 tggcttgcat atcgcccagt caggtcaaat ccctaaactg gttaccaagg tggaaaaaat
    51301 cacttttagc gccgcctacg cacagcaaag tggtcaggaa gtgttgtata tcactgaacg
    51361 cgcagtattc cagttaacgg cagaaggcgt tgaattaatt gaaatcgcgc cgggtgtgga
    51421 gattgagcgc gacattctgc cgtatatggc tttccgtcca attatcaagc acccacgcct
    51481 gatggaaagt agcctgttta cgccgatgga ggatgcatga gtcagcttga tgacaccatc
    51541 cttgatgcgc tgacgcacgt tactttccca aagggttttg cacaggcaga gcccgcatgg
    51601 gttgtcacgg tagacggtgt taattaccca ctctggcaaa cagatgccct ggtagtcggc
    51661 agtggtgcag caagattacg tgcggcagtt gaacttaaac gccgccagca aaatgtgctg
    51721 atcgctaccg ccgggttata tatggggacg tcggcatgtt ctggttccga taaacagaca
    51781 ctatttaccg ccgctaccgc gggcaacggc gacaacttca ccaaactggc agaggcactg
    51841 gcgagcggtg gggcgatgga tcacgacacc gcttatgtcg aagcggtagg ttctctgcac
    51901 actctaggcg ggttgcaata tcttggtctg gaattaccgg aagatcgcta tggcgcgatt
    51961 cttcgttatc aaaccgacca tgacgaagcc gggcgtgcaa cctcgtgtgg gctgcgtacc
    52021 tcaaggttaa tggtgaaagt gctgttggaa gaagtacagc gcctcgccat tccagtgttg
    52081 accagtgcaa cagtgattaa actgctgcat cagcgtgacg aaaacggcga agaccgcgtg
    52141 gcaggggcaa tcctcgcgac cggtcatcgc gcccataacc catgggggct ggcaattgtg
    52201 actgcgccca atgtggtact ggcaacgggc ggaccgggcg agctttatcg cgacagtgtt
    52261 tatccacata aatgcttcgg ctcgctgggg ctggcgctgg aggaaggtct gacgctaacc
    52321 aatctgaccg aaagccagtt tggtataggc acgccgcgta gcacgtttcc gtggaattta
    52381 tccggcacct atgtacaggt gatcccgtat atctattccg tggatgctga gggtaacgag
    52441 tataacttcc tcgcggatta ctatcgcacc acccaggagc tggcttcaaa cattttccgt
    52501 aaaggctatc agtggccgtt ccacgccact cgggtgatgg attttggctc cagcttgtta
    52561 gatatggcag tagcgcaaga gcagcaatca gggcgtcagg tatttatgga tttcaatcgc
    52621 aatcctgaag cggtgccggg tgacctgccg ttctcattag atcgactgga cgacgacgtt
    52681 cgcgcgtatc tggaaaataa cgacgctctg gcaccatcgc ccatcgaacg gctgcaacga
    52741 atgaatccgc tgtctatctc gctgtataag atgcacggtt acgatctcac cacacagcct
    52801 ttgcagtttg ccatgaataa tcagcatatg aatggcggca ttgaagtgga tatctgggga
    52861 caaacctccc tgcccggctg tttcgccgtg ggggaagttg ctggcacaca cggcgtcact
    52921 cgccctggtg gtgcagcatt gaatgccggg caggtttttg ctgttcgtct ggcacgtttt
    52981 attggttgca cgcaaaagcg gtctgttgag ggtgatgtcg cgcagctggt agcctcatct
    53041 ctgaatatag tcagagaagt tatcgctcag gcactggcaa acgagaacgg aatggcgctg
    53101 gtaacggtga aagacaaaat ccaggcgcga atgtctgatt acgccggatt tatttgtcat
    53161 gccgataaag ttcgtcatgc cacccgtgac gccctgctgc taagtgagtt tgtacaacaa
    53221 cacggtttaa ccatcaatca cgcaggcgaa attgccgaac tgtttatgtg gcggcatatg
    53281 acgctgactt ctgccgccgt attaacgcaa ttgacgcatt atattgacgc aggtggtggc
    53341 agtcgtggag cacggatggt tattgatcca caaggcaaat gcctcccaca aactcgtcgc
    53401 ggcgcaaaag aagaatggcg ttttcgctct gaacgtgctg aagacaaaaa tcacagatta
    53461 acgattcaat attcgcaagg ttcttttatt accgaagtga agtctttacg tatgcaaccg
    53521 tgtattaacg gtatttactt tgaaaaa
//
LOCUS       DAJTNA010000005        53336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTNA010000005 DAJTNA010000000
VERSION     DAJTNA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53336
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.005"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..173)
                     /gene="fadM"
                     /locus_tag="OO161_000228"
     CDS             complement(<1..173)
                     /gene="fadM"
                     /locus_tag="OO161_000228"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5461432.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNIN"
     gene            complement(267..638)
                     /locus_tag="OO161_000229"
     CDS             complement(267..638)
                     /locus_tag="OO161_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5461433.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(789..2660)
                     /gene="ppiD"
                     /locus_tag="OO161_000230"
     CDS             complement(789..2660)
                     /gene="ppiD"
                     /locus_tag="OO161_000230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5461434.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQVAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDLISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2852..3124)
                     /gene="hupB"
                     /locus_tag="OO161_000231"
     CDS             complement(2852..3124)
                     /gene="hupB"
                     /locus_tag="OO161_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5461435.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(3333..5687)
                     /gene="lon"
                     /locus_tag="OO161_000232"
     CDS             complement(3333..5687)
                     /gene="lon"
                     /locus_tag="OO161_000232"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5461436.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5875..7149)
                     /gene="clpX"
                     /locus_tag="OO161_000233"
     CDS             complement(5875..7149)
                     /gene="clpX"
                     /locus_tag="OO161_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5461437.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVINGQSKPLLIYGKPEAQQASGE"
     gene            complement(7275..7898)
                     /gene="clpP"
                     /locus_tag="OO161_000234"
     CDS             complement(7275..7898)
                     /gene="clpP"
                     /locus_tag="OO161_000234"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5461438.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(8144..9442)
                     /gene="tig"
                     /locus_tag="OO161_000235"
     CDS             complement(8144..9442)
                     /gene="tig"
                     /locus_tag="OO161_000235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5461439.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9786..10103)
                     /gene="bolA"
                     /locus_tag="OO161_000236"
     CDS             complement(9786..10103)
                     /gene="bolA"
                     /locus_tag="OO161_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5461440.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIV"
     gene            10408..10986
                     /locus_tag="OO161_000237"
     CDS             10408..10986
                     /locus_tag="OO161_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5461441.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            11030..12505
                     /gene="ampG"
                     /locus_tag="OO161_000238"
     CDS             11030..12505
                     /gene="ampG"
                     /locus_tag="OO161_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5461442.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12966..13913
                     /gene="cyoA"
                     /locus_tag="OO161_000239"
                     /pseudo
     CDS             12966..13913
                     /gene="cyoA"
                     /locus_tag="OO161_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
     gene            13935..15926
                     /gene="cyoB"
                     /locus_tag="OO161_000240"
     CDS             13935..15926
                     /gene="cyoB"
                     /locus_tag="OO161_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR5461443.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15916..16530
                     /locus_tag="OO161_000241"
     CDS             15916..16530
                     /locus_tag="OO161_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5461444.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSDFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            16530..16859
                     /locus_tag="OO161_000242"
     CDS             16530..16859
                     /locus_tag="OO161_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5461445.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16871..17761
                     /gene="cyoE"
                     /locus_tag="OO161_000243"
     CDS             16871..17761
                     /gene="cyoE"
                     /locus_tag="OO161_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5461446.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSVIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17910..19268
                     /locus_tag="OO161_000244"
     CDS             17910..19268
                     /locus_tag="OO161_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5461447.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQLPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IR"
     gene            complement(19402..19893)
                     /gene="yajQ"
                     /locus_tag="OO161_000245"
     CDS             complement(19402..19893)
                     /gene="yajQ"
                     /locus_tag="OO161_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5461448.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            20061..20972
                     /gene="panE"
                     /locus_tag="OO161_000246"
     CDS             20061..20972
                     /gene="panE"
                     /locus_tag="OO161_000246"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5461449.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAINCVINPLTAIWNCPNGELRHHPQEIMQVCEEVAAVIERE
                     GHHTSAEDLRHYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            20935..21525
                     /gene="yajL"
                     /locus_tag="OO161_000247"
     CDS             20935..21525
                     /gene="yajL"
                     /locus_tag="OO161_000247"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5461450.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(21579..23027)
                     /gene="thiI"
                     /locus_tag="OO161_000248"
     CDS             complement(21579..23027)
                     /gene="thiI"
                     /locus_tag="OO161_000248"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5461451.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            23233..23475
                     /gene="xseB"
                     /locus_tag="OO161_000249"
     CDS             23233..23475
                     /gene="xseB"
                     /locus_tag="OO161_000249"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5461452.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            23475..24374
                     /gene="ispA"
                     /locus_tag="OO161_000250"
     CDS             23475..24374
                     /gene="ispA"
                     /locus_tag="OO161_000250"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5461453.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLNALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            24399..26261
                     /gene="dxs"
                     /locus_tag="OO161_000251"
     CDS             24399..26261
                     /gene="dxs"
                     /locus_tag="OO161_000251"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5461454.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDDALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            26316..27278
                     /gene="yajO"
                     /locus_tag="OO161_000252"
     CDS             26316..27278
                     /gene="yajO"
                     /locus_tag="OO161_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5461455.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPARGRLTRP
                     WGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSKPGIA
                     APIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(27332..27850)
                     /gene="pgpA"
                     /locus_tag="OO161_000253"
     CDS             complement(27332..27850)
                     /gene="pgpA"
                     /locus_tag="OO161_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5461456.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(27828..28805)
                     /gene="thiL"
                     /locus_tag="OO161_000254"
     CDS             complement(27828..28805)
                     /gene="thiL"
                     /locus_tag="OO161_000254"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5461457.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRFGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(28883..29302)
                     /gene="nusB"
                     /locus_tag="OO161_000255"
     CDS             complement(28883..29302)
                     /gene="nusB"
                     /locus_tag="OO161_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5461458.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(29322..29792)
                     /gene="ribE"
                     /locus_tag="OO161_000256"
     CDS             complement(29322..29792)
                     /gene="ribE"
                     /locus_tag="OO161_000256"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5461459.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(29881..30984)
                     /gene="ribD"
                     /locus_tag="OO161_000257"
     CDS             complement(29881..30984)
                     /gene="ribD"
                     /locus_tag="OO161_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5461460.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVDAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(30988..31437)
                     /gene="nrdR"
                     /locus_tag="OO161_000258"
     CDS             complement(30988..31437)
                     /gene="nrdR"
                     /locus_tag="OO161_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5461461.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            31588..32127
                     /locus_tag="OO161_000259"
     CDS             31588..32127
                     /locus_tag="OO161_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5461462.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            32426..33310
                     /gene="tsx"
                     /locus_tag="OO161_000260"
     CDS             32426..33310
                     /gene="tsx"
                     /locus_tag="OO161_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5461463.1"
                     /translation="MKKTLLAAGVVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(33487..33834)
                     /locus_tag="OO161_000261"
     CDS             complement(33487..33834)
                     /locus_tag="OO161_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5461464.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(33963..34934)
                     /gene="secF"
                     /locus_tag="OO161_000262"
     CDS             complement(33963..34934)
                     /gene="secF"
                     /locus_tag="OO161_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5461465.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(34945..36792)
                     /gene="secD"
                     /locus_tag="OO161_000263"
     CDS             complement(34945..36792)
                     /gene="secD"
                     /locus_tag="OO161_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5461466.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQSVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(36820..37152)
                     /gene="yajC"
                     /locus_tag="OO161_000264"
     CDS             complement(36820..37152)
                     /gene="yajC"
                     /locus_tag="OO161_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5461467.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(37175..38302)
                     /gene="tgt"
                     /locus_tag="OO161_000265"
     CDS             complement(37175..38302)
                     /gene="tgt"
                     /locus_tag="OO161_000265"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5461468.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMD
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(38358..39428)
                     /gene="queA"
                     /locus_tag="OO161_000266"
     CDS             complement(38358..39428)
                     /gene="queA"
                     /locus_tag="OO161_000266"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5461469.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            39521..40102
                     /gene="acpH"
                     /locus_tag="OO161_000267"
     CDS             39521..40102
                     /gene="acpH"
                     /locus_tag="OO161_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR5461470.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALETRFWQFYPRMMAQASHKAL"
     gene            complement(40107..41829)
                     /gene="malZ"
                     /locus_tag="OO161_000268"
                     /pseudo
     CDS             complement(40107..41829)
                     /gene="malZ"
                     /locus_tag="OO161_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(41985..43358)
                     /gene="proY"
                     /locus_tag="OO161_000269"
     CDS             complement(41985..43358)
                     /gene="proY"
                     /locus_tag="OO161_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5461471.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(43434..44753)
                     /gene="brnQ"
                     /locus_tag="OO161_000270"
     CDS             complement(43434..44753)
                     /gene="brnQ"
                     /locus_tag="OO161_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5461472.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGITPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(45160..46455)
                     /gene="phoR"
                     /locus_tag="OO161_000271"
     CDS             complement(45160..46455)
                     /gene="phoR"
                     /locus_tag="OO161_000271"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5461473.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRIMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(46513..47202)
                     /gene="phoB"
                     /locus_tag="OO161_000272"
     CDS             complement(46513..47202)
                     /gene="phoB"
                     /locus_tag="OO161_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5461474.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            47392..48594
                     /gene="sbcD"
                     /locus_tag="OO161_000273"
     CDS             47392..48594
                     /gene="sbcD"
                     /locus_tag="OO161_000273"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5461475.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            48591..51734
                     /gene="sbcC"
                     /locus_tag="OO161_000274"
     CDS             48591..51734
                     /gene="sbcC"
                     /locus_tag="OO161_000274"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5461476.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            51860..53044
                     /gene="araJ"
                     /locus_tag="OO161_000275"
     CDS             51860..53044
                     /gene="araJ"
                     /locus_tag="OO161_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5461477.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(53187..>53336)
                     /locus_tag="OO161_000276"
     CDS             complement(53187..>53336)
                     /locus_tag="OO161_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5461478.1"
                     /translation="MSNVDRLYQTVPQLIKQFVFGGECETPVRKAKHGDSSGVRGAAW
                     LWPQE"
BASE COUNT        12704 a        14284 c        13379 g        12969 t
ORIGIN      
        1 atattgatat tgaccacgac gaaggcgatg ttatgggccg tcatccactg aaaactgtcg
       61 ctattttcca acccatccca gcgggcttct tcgagaaatt caaggtagcg ggcgttgttg
      121 acgtgctggt aaacgtcgag atgatatcca cgaactttga tttgtgtttg catagcgcaa
      181 taaccttacg ttgttgttat aagtacagag gtcataactg gtatgacctc ttcagtctgg
      241 caaaaaattg ccactatgca aattaattac agggttaata ccgccagatt acgttccacc
      301 agcgaattgc ccatccccgg cacctgcttt agatcctcca ctgttttaaa cggaccgtac
      361 tcttcgcgat aactgacaat cgcctgcgct ttcttcaggc caacgccatt catcgcgcgg
      421 gctagctctt ccgcgctggc attattaatg ctgacccggg tgccttcttc gtcactggct
      481 ttcgccggta ctgctgcttt actttgtgct gcaggagctt ccgctttggt ttctaccgcc
      541 gtcggtttcg ccacagaagc tgccgccagc gcgctatgag acattccggc acaggccagg
      601 gacagggtaa tgagcagtgc tttaattccg tgtttcatac tgttttctcc ttgtttgtta
      661 acagtggaat caccttaacg gcagtgaaaa cagtaaacaa atggcaatgt tcagaaatgg
      721 aaaaggccgc gaaagcgacc ttttcttgtt acagctgcaa cgcatttttt tgcgaggcgt
      781 cttcaggatt attgctgttc cagcgcatcg ccaattttga ttttcgcctc tttacgcagg
      841 ttactcatca gagcttcaaa gacgatttgt gcgttgttct gggtgatacc ctgcaccatc
      901 gcttttttct gatcttccgg cattgaacct tgtttcactt catccagcgc cagcagaacc
      961 acattacctt gcatatcggt cgccataccg tagctcggtt tgtctttcgc tggcagtggc
     1021 agtgcaaacg ccgcctggct aatcaggtca cgaccggaac ggcttaaggt tttcggctcg
     1081 ccaaatttca gaccggcagc ctgcatagct tccgcacctt tgccggcttt caaatcaacc
     1141 agcagtttct cagcatccac tttcgcctgt tgttcagctt tgttgtgctg aaccagtgcc
     1201 ttaacttgtt cctgaacatc tgccaacggt ttcaccgctt ccggtttgtg ctcgctgatg
     1261 cgcagcacga atgcacggtc gccgtctacg gtgatgatgt cagagttgat gcccggcgcg
     1321 ccgttttcac ctaccagacc gccgttaaag atagcgtcgg caaccggctt gaagttcaac
     1381 tcttccggca ggttatcttt gctgaaccaa cccgtctgag tggctttaac gccggcaact
     1441 tgctctgcac cggccagaga ctcggtgtcg ttgcttgccg catcgctcac tttctgctgc
     1501 agcgcatagt acgcatcgag ggctttttcg tgtttcactt tcgccgcaat gtcgtcacgt
     1561 acttcgtcta acgatttcac tttcgctggc tgaatgtcgt ccagacgtac aatcaggaaa
     1621 ccgaccgaag atttgatgac accagacagt tggccttttt ctttcagacc agcatttttc
     1681 agttcgtcgg ggatagtggc atcttctaac caacccatat cgccgccgtt acgggcagag
     1741 ataatatcgg cagatttttc tttggctaat gcagcaaaat caccgccttt attcagctca
     1801 tcaagtaccg ctttcgcttc atcttcagtt ttggtctgga tgatgctgta gcgggtacgc
     1861 tgcggctggg tgaattgatc ctgatgctga tcgtagtagc tctggatatc cgcatcgcta
     1921 accggttgct gcatcgttgc ggcatccagc ttgatgtaac tcacgcggaa ttgttccggc
     1981 gtcatgaaat tgtttttgtt ttgttcgtag tagctggcaa tctcctgttc ggtcacaggc
     2041 tgcttcgccg ccagcgcgtt aacatcgata gtcgcctcac gcaccacgcg ttgttgcgcg
     2101 accagtgccg ccagctcgtc ggtttcacct ttcagcataa aatcggtacc ggcaacaccg
     2161 ttaatcagct gttgggtagt gagctggtta cgcagcgcct gggcgtactg atcggcggtc
     2221 atccccatct ggttgaggat accgttatag cggctgttat caaatttgcc gtcaacctgg
     2281 aaggctgggg tcgcgaaaat cgcctgttta acctgctcat cactgatacc cagtttcagc
     2341 tcacgagcgt actgatccaa cagcgcctcg tcgatcagac gattcagcac ctgttgacgc
     2401 agggttttca tatagccttc attcgctgcc agctcggagt attgatcgcc cagctgttgc
     2461 tgcatgcgat tacgctcgct gttaaaggcg ttctcgaact gcccacggct gatttcctgg
     2521 tcattcactt ttgcggcgta gttattgcct ccgccaatca ggtaaccact cacgccggtc
     2581 aatatgaacg acacgataat gataccgaaa ataatcttga gcacgagact gtttgcagcc
     2641 gtgcgtaagc tgtccatcat ggtgtaacaa cactccgctg tagatgactg tgtacctcac
     2701 gcaacgtatc gatgcgcgca aaggcctatt gtgacaagaa acgggggcaa ttgtcagccc
     2761 acaagacgca gaaagtcacc gaatacaaat aaaaaaggca catcagtaga tgcgcccttg
     2821 aacttcgtca catccccacc ggggacaacg cttagtttac cgcgtctttc agtgctttac
     2881 ctgcacggaa gctcggtact ttagcagcag cgatggtgat ctctttaccg gtctgcgggt
     2941 tgcggccagt acgggcagca cgctctttaa cggcaaaagt accaaaacct accagtgcta
     3001 catcatcccc ttctttcaga gattcagtta cggaagcaat aatagcatct aacgcacggc
     3061 cagccgcagc tttagagata tcagcccctg cagcaatctt gtcgatcaat tgagatttat
     3121 tcactcttct cttcctcttt ataatttata tcgcacttga atccttcaag gtacgaacgc
     3181 gcagcagtta tatcaggcca gccatcccct tacaacaccc cttaataagg gcaagcccga
     3241 tccgccatct aacttagcga gacaaaaaaa ggctggcaag cccgaattag cctgccagcc
     3301 ctgtttttat tagtgcattt tgcgcgaggt cactattttg cagtcacaac ctgcatgcca
     3361 gacggttcat tttgcagcgc cagagtcaga acttcctcaa tgcgcttcac aggatgaatg
     3421 tccagatcgg caattacgtt gtcaggaatc tcttccagat cgcgtttatt ttcgaacgga
     3481 attagcactg ttttaatccc gccgcgatgc gctgccagga gtttttcttt caaaccaccg
     3541 atcggcagta cctgaccacg cagagtgatc tcaccggtca ttgccacatc ggcacgaacc
     3601 gggttaccgg tcaggcaaga aaccagcgcg gtgcacatag caataccggc actcggacca
     3661 tctttcggcg tcgcaccttc cggtacgtgg acgtggatgt cacgtttttc gtaaaaatca
     3721 gggttgatcc ccagtttttc cgcacgcgca cgaaccaccg ttaacgccgc ctgaatagac
     3781 tcctgcatca cttcgccgag cgaaccggta taggtcagtt tgcctttacc cggaacacat
     3841 gcggtttcaa tggtcagcaa gtcaccgccc acttccgtcc acgccagacc ggttacctga
     3901 ccgacacagt tttcgttatc agcgcgacca tagtcgaaac gctgaacacc gaggtagtca
     3961 tgcaggttat cgccgttaat ttcgatatgt tttaatgact tatcgagcag taactgctta
     4021 accgctttgc ggcacagttt ggagatttca cgctccagac cacgcacgcc cgcctcacgg
     4081 gtgtagtaac gaataatgcc gataatggcg ctatcgtcga cggtcagctc accttttttc
     4141 agtgcattac gttcaatctg cttcggcagc aggtgacgtt tggcgatgtt cagtttttca
     4201 tcttcggtat aaccggagag gcgaatcact tccatacgat ccagcagcgg tgccggaatg
     4261 ttcatggagt tcgacgtcgc gacaaacatc acgtcgctga gatcgtaatc cacttccagg
     4321 tagtggtcgc tgaacgctac gttctgctct ggatccagca cttcaagcag tgcagaggcc
     4381 ggatcgcctc gcatgtcaga agacattttg tcgatctcat cgagcaggaa cagcgggttt
     4441 ttcacgccca ctttcgccat tttctggatc agtttacccg gcatagaacc gatgtaagta
     4501 cggcggtgac cacggatttc cgcttcatca cgcacgccgc ccagcgccat acggacatat
     4561 ttacgcccgg tggctttggc aatggactga ccaagagagg ttttacctac ccccggcggc
     4621 cctaccaggc agaggatcgg tcccttgatt ttgttgacac ggctttgaac cgcaaggtac
     4681 tcaaggatgc gatctttcac gcgctccaga ccataatggt cggtatcaag gatttcctgc
     4741 gcctgacgca ggtctttttt gaccttgctg cgcgcattcc acggtacctg taccatccag
     4801 tcgatataac cacgcactac ggtcgcttct gccgacatcg gagacatcat tttcagcttc
     4861 tgcaactctg cttccgcttt ctcttttgcc tctttcggca tcttcgccgc gtcgattttg
     4921 cgcttcaggg cttcgttttc gtccggcgcg tcgtccattt caccgagttc tttctgaata
     4981 gctttcattt gctcgttcag atagtactca cgctgggatt tctccatctg ctttttaacg
     5041 cggttgcgaa tgcgtttctc aacctgcagc agatcgattt ccgattccat cattgccatc
     5101 agatattcca gacgttcgtt aacgtcggac atctccagaa cggactgttt gtcagccagt
     5161 ttcagtggca tatgtgcagc aatggtatcc gccagacgcg ccggatcgtc gatgctattc
     5221 agcgacgtca gcacttctgg tgggattttt ttgttcagct tgatgtagcc ttcgaactgg
     5281 ctgattgcag tacgcaccag cacttcctgt tcccgctcat caatggtcgg cgactccaga
     5341 tactccgcct tcgcagaaaa gtgttcgcca ttgtcagaga gcgcagaaat acgcgcgcgc
     5401 tgtaacccct cgaccagcac tttgacggtg ccgtcaggca gtttcagcat ctgcaatata
     5461 gaggccacgg tcccgacggt gaaaagatcg tttacacccg gctcatccgt tgaagcttct
     5521 ttctgcgcga ccagcataat ttttttatca tggtccatcg ccgcttccag acaacggata
     5581 gatttttccc gcccgacaaa taaggggatg accatgtgcg gataaaccac cacatcgcgc
     5641 agcggcaata cggggatttc aatgcgttca gaacgctcag gattcataga gctctctctt
     5701 agtttaattt ccgccaggta atcagatgac acgactgtgc ttcacgccat ctattaacat
     5761 gtacgtcagt atatggggat gtttccccca cattcaacgc cgagaataga ggaaaaatta
     5821 aaggggagat aaaatccccc ctttttggtt aactaattgt atgggaatgg ttaattattc
     5881 accagatgcc tgttgcgctt ccggcttacc ataaatcagc aacggtttgc tttgaccatt
     5941 aattaccgac tcgtcgataa ccactttttc gacatcttcc atggacggca gatcgtacat
     6001 ggtatcgagc agtgcggctt ctacgatgga acgcaggcca cgggcaccgg ttttacgcgc
     6061 catcgctttc ttagcgatag catccagcgc ctcgtcacgg aattccagat ccacgccttc
     6121 cagattaaac agcgcctgat actgcttggt cagggcgttt ttcggctctt tgaggatctg
     6181 aatcagagct tcttcgctca gttcattcaa cgttgcgaca accggcagac gaccaataaa
     6241 ctcagggata agaccaaact tgatcagatc ttccggttca acctgcgcca gcagctcgcc
     6301 ttcgcttgct ttgtcggact tcgcttttac cgtcgcgcca aaaccaatgc cggaaccggt
     6361 ttctacacgg tgggaaatca ctttatccag accggcaaac gcaccgccac aaataaacag
     6421 gatcttggag gtatcaacct gcaaaaattc ctgctgcgga tgcttacgcc cgccctgcgg
     6481 tggaacggca gcaacagtac cttcgatcag tttcaacagt gcctgctgta cgccttcacc
     6541 ggaaacgtca cgggtaatag acgggttgtc cgacttacga gaaatcttgt cgatttcatc
     6601 gatgtagaca ataccacgct gtgctttctg gacatcgtag tcgcatttct gcaacagctt
     6661 ctgaatgatg ttttcaacgt cttcacccac ataaccggct tcggtcagtg tagtcgcgtc
     6721 ggccatggtg aacggaacat ccagcaggcg cgccagcgtt tcagccagca gcgttttacc
     6781 ggaaccggtc ggaccgatca gcagaatgtt acttttgccc aactcgacac cattgctggt
     6841 atcgccgttg cgcagacgtt tgtaatggtt gtataccgcg accgccagca cttttttcgc
     6901 ctgttcctgg ccgataacgt aatcgtccag gtggttgcga atttcatgcg gcgtcggtag
     6961 cgcactgcgt tcacgatgcg gtgcaacttc tttaatctct tcgcgaatga tgtcgttaca
     7021 taaatcaaca cattcgtcgc agatatacac ggatggaccg gcaatcagct tgcgcacttc
     7081 atgctggctt ttgccgcaaa aagagcaata cagcaatttg cctgagccat ctttgcgttt
     7141 atctgtcatg ggtcaaaacc tcttctttgt tctttgtgcc gcacacgacg acgcaaatgc
     7201 cattctcagg cgcaagccgc ttacagcgtt gtgccgccct ggataagtat agcggcacag
     7261 ttgcgcctct ggcatcaatt acgatgggtc agaatcgaat cgaccagacc gtattccacc
     7321 gcttcagggg cggaaaggaa gcgatcgcgc tcggtatcac gttcaatctg ttctaatgat
     7381 tgacccgtat gaagcgccat aagttcattc atgcgccctt taactttcag aatttcacgg
     7441 gcatgaattt cgatatcggt cgcctggccc tggtagccgc ccaacggttg gtgaatcatc
     7501 acgcgcgaat tcggcaggca aaaacgttta ccttttgccc ctgcggtcag caagaaagcg
     7561 cccatcgagg ccgcctggcc catacagatg gtgctgacat caggcttgat aaactgcatg
     7621 gtgtcataga tagacatccc ggcagtgatc accccgcctg gggagttaat gtacagatag
     7681 atatcttttt ctgggttttc cgcttccagg aacagcatct gcgccacaat caggttagcc
     7741 atgtggtctt caacctggcc agtcagaaaa atgacgcgtt ccttaagtag acgagaatag
     7801 atatcaaaag agcgctcacc gcgtgaggtc tgttcaatga ccatcggcac cagcgccata
     7861 tggggtgcaa agttatctcg ttcgccgctg tatgacattt ccgtctcctg gataaaattg
     7921 aaaaaacctg ctgtaccgat tgtaacctta tggacggatt atcagctagt ccctgtgtta
     7981 tgggtacggc atcaccatat ttcaagcata acaatctttt gttgttacgc taacaccgaa
     8041 agaggctttt cgcacggttc catgcaaaat tcatgacaaa aaaaagccca ccacctggcg
     8101 gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt aaattacgcc tgctggttca
     8161 tcagctcgtt gaaagtggtt tctttttcag tcactttcgc tttcgccagt acagcttcaa
     8221 cagcctgttc ttccagagca acattgcgca tgttgtccat cagttctttg tttttgctgt
     8281 agaactcgat aacttctttc ggatcttcgt acgcagaagc catctcttcg atcaggcctt
     8341 tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat aacttcgccc agcagcaggc
     8401 caacaactac gcggcgttta gcctgttctt cgaacagttc gcgcggcagt tccagagctt
     8461 gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg cagaacgtcg atttcgctgt
     8521 cgatcagcgc agccggtacg tcgatgtcgt tagcttttac cagaccttcg atcgcctgag
     8581 acttaacgcg gttacggatg gcgctcttca gctcgcgctc catgttttta cgcacttcag
     8641 cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg tttgatgaat tctgcagtca
     8701 gttccggcag ttcacgctct tcaactttct tcaggttgat agcgaatttc gctgctttac
     8761 ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac gtcgatggtg aactcttcgc
     8821 cagctttgtg gcctttgata ccgtcttcaa agcccgggat catacgaccc tggcccatcg
     8881 ccagtacgaa atcagacgct ttaccgcctt cgaactcttc gctgtctaca gaaccggtga
     8941 agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc tttttctttc caggtcgcct
     9001 gctgtttacg cagagtatcc agcatgccgt caacgtcagc gtcggtcact tcaacgatcg
     9061 gtttttcaac ttcgatcgct tccagaccct gcagttcaac ttccggataa acttcaaact
     9121 ctacagagta agtgaagtct tcacccagct tgtattcgcc cggaacataa gtcggtgcgc
     9181 cagccggatt gattttttct ttaatgatgg cgtcaatgaa gttacggctc atcaggtcac
     9241 ccagaacgtc ctggcgtaca gacgcgccat aacgctgagc aacgatattc attggcactt
     9301 tgcctttgcg gaagccgtca atacgtactt ttttcgcaac gttgaccagc tcgcttttaa
     9361 cagcggtctc gatgctatca gcagcgatag taatcgttac acggcggcca aggccttgag
     9421 tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat cacagtgctc ggtcaactct
     9481 taccccgcaa gcacaggttg ttggcttcgc ggaaccggga tgttcttata atcaatcaca
     9541 ttccctgtta ccagaatcat cccgaaagca ttcaaatagg acacggcatt atagcggcat
     9601 cacttttatg agtcgagaac ggttattgcg cgctgtcgcg tattttttca caattcccgg
     9661 gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc agacaaaaca aaacggcccg
     9721 caggccgttt ttcataaaat ctttagcaac atactgaaaa agcgccgaca gttgcaaatg
     9781 cgtttttaca cgatgcttcc tgctccacga cagggaggag aggcaaagac ggtgtcctgc
     9841 aacccttccc actccttaat agtgtaagta tgcagagcca gcgcatgaac ggtagtagag
     9901 agttcctccg ctaaagtact gtaaatcatt cgatgacgat tcagaaaacg ttcacccgta
     9961 aaacgatcgc tgaccagcac aactttaaaa tggctttcag agccggctgg gacattgtga
    10021 cgatagcttt catccactac ttcgaggaat acgggttgga acgccgccct taatttttct
    10081 tctatccgct cacgtatcat catgaaatat ctcctccgac aacgctgaga tgtcatccat
    10141 ccctttaaat actagccgct tttaccgttt ccattgcagc ttaacaacaa atatttaggt
    10201 ttcgtcctgg tttttccgtc aagcgtatga agtttactga tttaaagatt gaaacacagg
    10261 cctcgcatca gcgtttcttt taccaggggt gattatttcg tcaacatgat gaatttacat
    10321 gccttaccca cttcccctcg ccgttggcaa tgttatgatg gcgggaattt ttaccaaaca
    10381 cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa tcctcttccc gttagttgct
    10441 ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta ttgaagtttc cccgacgatt
    10501 acactgccac agcaggatcc aagcctgatg ggcgtcaccg taagcattaa tggtgccgat
    10561 cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata atcaaatcgt taccctgacc
    10621 gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg aaaaacagat gaccgcgcgt
    10681 ggttacatgg ttggtccgaa tggcccggtt aatctgcaaa tcatcgttag ccaactgtat
    10741 gctgacgtgt cccagggcaa cgtgcgctac aacatcgcta ccaaagcgga tatcgccatc
    10801 atcgctaccg cgcagaatgg caacaaaata accaaaaact atcgtgccag ctacaacgtt
    10861 gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg cggttaacag tgtgctgacc
    10921 gataccatcg ctgatatgtc tcaggacacc agcattcacg aattcatcaa gcagaacgcg
    10981 cgttaatttc tgccctctgg cccggtgcaa accgggcctg tagacgccca tgtccagtca
    11041 atatttacgt atttttcaac agccgcgttc agccatattg ctgatcctgg gttttgcttc
    11101 cgggctaccg ctcgccctga catccggtac cttacaggcc tggatgacgg tcgagaatat
    11161 tgatctcaaa accattggtt tcttctctct ggtaggccag gcttacgttt ttaaattcct
    11221 ctggtcaccg ctgatggacc gctacacgcc cccatttttt gggcggcggc gcggttggct
    11281 gctcgccacg caaatcctgt tattagtcgc cattgcggcg atgggttttc tcgaacctgg
    11341 cacccaactc cgctggatgg cggcactggc ggtggtgatc gctttttgct cggcctccca
    11401 ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg gcagaagaac gtggtgcggg
    11461 cgcggcaatc agcgtgctgg gttaccgttt agggatgctg gtttccggcg gcttagccct
    11521 gtggctggca gataaatggc tgggctggca gggcatgtac tggttgatgg cggcgctgtt
    11581 gatcccttgt attatcgcga cgttgcttgc accagaaccg accgacacaa ttcctgtgcc
    11641 aaaaacgctg gaacaagcgg ttgttgcacc tctgcgagat ttctttggtc gcaataatgc
    11701 ctggcttatt ttgcttctta tcgtgctgta taagctgggc gacgcattcg ccatgagcct
    11761 gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg ggtgaagtag gcgtggttaa
    11821 caaaacgctt ggcttactgg cgaccattgt gggcgcattg tacggtggga ttttgatgca
    11881 gcgcctgtcg ctgttccggg cgctgctgat tttcggcatt ttacaaggtg cgtctaacgc
    11941 tggttactgg ctgctgtcga ttactgataa gcatctctac agcatgggcg cagccgtctt
    12001 tttcgaaaac ctctgtggcg ggatgggcac atcagccttt gtcgcgctgt taatgacgct
    12061 gtgtaataag tcattttccg ctactcaatt tgccctgctc tcagcgcttt ccgctgtagg
    12121 acgagtttat gtcggccccg tggcgggttg gtttgttgaa gcacacggct ggtcgacatt
    12181 ctatctattc tccgtcgccg ctgccgtacc ggggcttatt ttgctgctgg tttgccgcca
    12241 gacgcttgaa tatacacgag taaatgacaa ctttatctcc cgtaccgaat atccggcagg
    12301 ttatgccttt gccatgtgga cactggcggc gggcgtcagc ctgttggccg tgtggttact
    12361 gctgttgacg atggacgcgc tggatttgac gcacttctct ttcctgcctg ctctgctgga
    12421 agtcggggtt ttagtcgccc tttctggcgt cgtgcttggt ggtttgctgg attatctggc
    12481 tctacgaaaa acgcatctaa cgtaatctgt aaatattatt tagctgtagt aatcactcgc
    12541 cgtaattatt acggataaat aaacatcagt atcgcttaat aatatttgtt cttttgtgcg
    12601 gcttagcgtt tggaaattat tgacgcaatt tatgaattct attttttctt acacgattca
    12661 gctaacgagt ctttattttc cccccaccca attgtcacca taatgataat tatttgttaa
    12721 acaattattt tattccagat tggttatacc aattgcccat ccaaactcct tctgcactcc
    12781 cctttcgtta taacgccctt ttgcaacagc ttcttaaaat caacttgata tgttttgcaa
    12841 catatgtgac ctgacagcca aatccaagta acagtaattt aatcatgttt acagtaatgt
    12901 aaccttcccg taaaatgccc acacacttta aacgccacca gatcccgtgg aattgaggtc
    12961 gttaaatgag actcaggaaa tacaataaaa gtttgggatg gttgtcatta tttgcaggca
    13021 ctgtattgct cagtggctgt aattctgcgc tgttagatcc caaaggacag attggtctgg
    13081 agcaacgttc actgatactg acggcatttg gcctgatgtt gattgtcgtt attcccgcaa
    13141 tcttgatggc tgttggtttc gcctggaagt atcgtgcgag caataaagat gctaagtaca
    13201 gcccgaactg gtcacactcc aataaagtgg aagctgtggt ctggacggta cctatcttaa
    13261 tcatcatctt ccttgcggta ctgacctaga aaaccactca cgctcttgag cctagcaagc
    13321 cgctggcaca cgacgagaag cccattacca tcgaagtggt ttccatggac tggaaatggt
    13381 tcttcatcta cccggaacag ggcattgcta ccgtgaatga aatcgctttc ccggcgaaca
    13441 ctccggtgta cttcaaagtg acctccaact ccgtgatgaa ctccttcttc attccgcgtc
    13501 tgggtagcca gatttatgcc atggctggta tgcagactcg cctgcatctg atcgccaacg
    13561 aacccggtac ttatgacggt atctccgcca gctacagcgg cccgggcttc tcaggcatga
    13621 agttcaaagc tattgcaaca ccggatcgcg ccgaattcga ccagtgggtc gcaaaagcga
    13681 agcagtcgcc gaacaccatg tctgacatgg ctgcgttcga aaaactggcc gcgcctagcg
    13741 aatacaacca ggtggaatat ttctccaacg tgaaaccaga cttgtttgct gatgtgatta
    13801 acaagtttat ggctcacggt aagagcatgg acatgaccca gccagaaggt gagcacagcg
    13861 cacacgaagg tatggaaggc atggacatga gccacgcgga atccgcccat taaaggggtt
    13921 gaggaagaat aaagatgttc ggaaaattat cacttgatgc agtcccgttc catgaaccta
    13981 tcgtcatggt tacgatcgct ggcattattt tgggaggtct ggcgctcgtt ggcctgatca
    14041 cttacttcgg taagtggacc tacctgtgga aagagtggct gacctccgtc gaccataaac
    14101 gcctcggtat catgtatatc atcgtggcga ttgtgatgtt gctgcgtggt tttgctgacg
    14161 ccattatgat gcgtagccag caggctcttg cctcggcggg cgaagcgggc ttcctgccac
    14221 ctcaccacta cgatcagatc ttcaccgcgc acggcgtgat tatgatcttc ttcgtggcga
    14281 tgcctttcgt tatcggtctg atgaacctgg tggttccgct gcagatcggc gcgcgtgacg
    14341 ttgcgttccc gttcctcaac aacttaagct tctggtttac tgttgttggt gtgattctgg
    14401 ttaacgtttc tctcggcgtg ggcgaatttg cgcagaccgg ctggctggcc tatccaccgc
    14461 tatcgggaat agagtacagt ccgggagtcg gtgttgatta ctggatatgg agtctccagc
    14521 tatccggtat aggtacgacg cttaccggta tcaacttctt cgttaccatt ctgaagatgc
    14581 gcgcaccggg catgaccatg ttcaagatgc cagtatttac ctgggcatca ctgtgcgcga
    14641 acgtactgat tattgcttcc ttcccaattc tgacggttac cgtcgcgttg ttgaccctgg
    14701 atcgctatct gggcacccat ttctttacca acgatatggg tggcaacatg atgatgtaca
    14761 tcaacctgat ttgggcctgg ggccacccgg aagtttacat cctgatcctg cctgttttcg
    14821 gtgtgttctc cgaaattgcg gcaaccttct cgcgtaaacg tctgtttggt tatacctcgc
    14881 tggtatgggc aaccgtctgt atcaccgtgc tgtcgttcat cgtttggctg caccacttct
    14941 ttacgatggg tgcgggcgcg aacgtaaacg ccttctttgg tatcaccaca atgattatcg
    15001 ccatcccgac cggggtgaag atcttcaact ggctgttcac catgtatcag ggccgtatcg
    15061 tgttccattc tgcgatgctg tggaccatcg gttttatcgt cactttctcg gtgggcggga
    15121 tgactggcgt gctgctggca gtaccgggcg cggacttcgt tctgcataac agcctgttcc
    15181 tgattgcgca cttccataac gttatcatcg gcggcgtggt cttcggctgc ttcgcaggga
    15241 tgacctactg gtggcctaaa gcgttcggtt tcaaactgaa tgaaacctgg ggtaaacgcg
    15301 cgttctggtt ctggatcatc ggcttcttcg ttgcctttat gccgctgtat gcgctgggct
    15361 tcatgggcat gacccgtcgt ttgagccagc agattgaccc gcagttccac accatgctta
    15421 tgattgcagc tagcggtgca gtactgattg cgctgggtat tctctgcctc gttattcaga
    15481 tgtacgtttc tattcgcgac cgcgaccaga accgtgacct gactggcgac ccgtggggtg
    15541 gccgtacgct ggagtgggca acctcttccc cgcctccgtt ctataacttt gccgttgtgc
    15601 cgcacgttca cgagcgtgat gcattctggg aaatgaaaga gaaaggcgaa gcgtataaaa
    15661 agcctgacca ctatgaagaa attcatatgc cgaaaaacag cggtgcaggt atcgtcattg
    15721 cagctttctc caccatcttc ggtttcgcca tgatctggca tatctggtgg ctggcgattg
    15781 ttggcttcgc aggcatgatc atcacctgga tcgtgaaaag cttcgacgag gacgtggatt
    15841 actacgtgcc ggtggcagaa atcgaaaaac tggaaaacca gcatttcgat gagattacta
    15901 aggcagggct gaaaaatggc aactgatact ttgacgcacg cgactgccca cgcgcacgaa
    15961 cacgggcacc acgatgcagg cggaaccaaa atcttcggat tttggatcta cctgatgagc
    16021 gactgcattc tgttctcaat cttgtttgct acctatgccg ttctggtgaa cggcaccgca
    16081 ggcggcccga caggtaagga cattttcgaa ctgccgttcg ttctggttga aactttcttg
    16141 ctgttgttca gctccatcac ctacggcatg gcggctatcg ccatgtacaa aaacaacaaa
    16201 agccaggtta tctcctggct ggcgttgacc tgtttgtttg gtgccggatt tatcgggatg
    16261 gaaatctacg aattccatca cctgattgtt aacggcatgg gtccggatcg cagcgacttc
    16321 ctgtcagcgt tctttgcgct ggtcggcacg cacggtctgc acgtcacttc cggtcttatc
    16381 tggatggcgg tgctgatggt acaaatcgct cgtcgcggcc tgaccagcac taaccgtacc
    16441 cgcatcatgt gcctgagcct gttctggcac ttcctggatg tggtttggat ctgtgtgttc
    16501 actgttgttt atctgatggg ggcgatgtaa tgagtcattc taccgatcac agcggcgcgt
    16561 cccatggcag cgtaaaaacc tacatgacag gctttatcct gtcgatcatt ctgacggtga
    16621 ttccgttctg gatggtgatg acaggggctg cctctccggc cgtaattctg ggaacaatcc
    16681 tggcaatggc agtggtacag gttctggtgc atctggtgtg cttcctgcac atgaatacca
    16741 aatcagatga aggctggaac atgacggcgt ttgtcttcac cgtgctaatc atcgctatcc
    16801 tggttgtagg ctccatctgg attatgtgga acctcaacta caacatgatg atgcactaag
    16861 agcggcggtt atgatgttta agcaatacct gcaagtaacg aaaccaggca tcatctttgg
    16921 caacctgatc tcggtgattg ggggattcct gctggcctca aagggcagca ttgattatcc
    16981 cctgtttatc tacacgctgg ttggggtgtc actggttgtg gcgtcgggtt gtgtgtttaa
    17041 caactacatc gacagggata tcgacagaaa gatggaaagg acgaagaatc gggtgctggt
    17101 taaaggcctg atctctcctg ctgtctcgct ggtgtacgcc accttgctgg gtattgctgg
    17161 ctttatgctg ctgtggtttg gcgcgaatcc gctggcctgc tggctggggg tgatgggctt
    17221 tgtggtttat gtcggcgtct atagcctgta catgaaacgc cactcagtct acggcacatt
    17281 gattggttcg ctctccggcg ctgcgccgcc ggtgattggc tactgtgcag taaccggcga
    17341 gttcgatagc ggcgcagcga tcctgctggc tatcttcagc ctgtggcaga tgcctcactc
    17401 ctatgccatc gccattttcc gctttaagga ttaccaggcg gcaaacattc cggtattgcc
    17461 agtggtaaaa ggcatttcgg tggcgaagaa tcacatcacg ctgtatatca tcgcctttgc
    17521 cgttgccacg ctgatgctct ctcttggcgg ttacgctgga tataaatatc tggtggtcgc
    17581 cgcggcggtt agcgtctggt ggttaggtat ggctctgcgc ggttataaag ttgctgatga
    17641 cagaatctgg gcgcgcaagc tgttcggctt ctctgtcatc gccatcactg ccctctcggt
    17701 gatgatgtcc gttgatttta tggtaccgga ctcgcatacg ctgctggctg ctgtgtggta
    17761 acaaaacctc tctattaaaa aggtgctacg gcaccttttt tcttagcatt agaaacatat
    17821 ccctctcgaa atatttacta aaaaatccgc atgtttaccc cattcgtttg ccgctttaca
    17881 ctagtcgcga atttaaaaca gaggtggtaa tgaacgatta taaaatgacg ccaggtgaga
    17941 ggcgcgcgac ctggggttta gggaccgtat tctcgttgcg catgctgggc atgtttatgg
    18001 ttctgccggt tctgaccacg tatggcatgg ctctgcaagg tgccagcgaa gcattaatcg
    18061 gtattgccat tggtatttat ggtctgactc aggccgtttt tcagcttccg tttggcctgc
    18121 tttccgaccg tattggtcgc aaaccattaa ttgtcggtgg gctggcggtg tttgccgccg
    18181 gtagcgttat cgctgcgctc tctgactcca tctggggaat tattctgggc cgggcgctac
    18241 aaggctccgg tgcgattgcc gctgccgtta tggcgctgct ttccgatctc acgcgcgaac
    18301 aaaaccgcac caaagcaatg gcgtttatcg gcgtgagctt tggcattacc tttgccattg
    18361 cgatggtgct tggcccgatc atcactcaca aacttgggct gcacgcgctg ttctggatga
    18421 tcgctattct ggcaacgacc ggcattgcgt tgaccatttg ggttgtgccc aacagtagca
    18481 ctcacgtact taatcgtgag tccggaatgg tgaaaggcag tttcagtaaa gtgctggcgg
    18541 aaccgcggct gctgaaactc aactttggca ttatgtgtct gcatattttg ctgatgtcga
    18601 cgtttgttgc cctgcccgga caactggctg atgcagggtt cccggcggct gaacactgga
    18661 aggtctatct ggcgacaatg ctaatcgcct ttggctcggt cgtgcctttc attatctacg
    18721 ctgaagttaa gcgcaaaatg aagcaagtct ttgtcttctg cgtcgggttg atcgtggttg
    18781 cggaaattgt gttgtggaac gcgcaaacgc agttctggca actggtggtc ggcgtgcagc
    18841 ttttctttgt agcgtttaat ttgatggaag cccttctgcc ttcacttatc agtaaagagt
    18901 cgccagcagg ttacaaaggt acagcgatgg gtgtttactc caccagccag tttcttggcg
    18961 tggcgattgg cggttcactg ggcggctgga ttgacggcat gtttgacggt cagggcgtat
    19021 ttctcgctgg cgcaatgctg gccgcagtgt ggctggcagt cgccagtacc atgaaagaac
    19081 cggcgtatgt cagcagtttg cgcattgaaa tcccggcgaa cattgccgca aacgaggcgt
    19141 taaaagtgcg tttgctagaa actgaaggca tcaaagaagt gttgattgca gaagaagaac
    19201 attcagctta tgtgaaaatc gacagcaaag tgacgaatcg ctttgatgtt gaacaggcaa
    19261 ttcgctaggc ataaaagcga ggcgaaattg cctgctgcgc tacgctcatc aggcctacta
    19321 agctcctgat ttacaagcct ttgtaggccg aaccaggcgt tcatgccgca tctgacatga
    19381 acaaagcgca ggttgtcgcg attaatcgcg gaagtttttg aactggaacg gctgacccag
    19441 atcgccaccg cgcaccatcg ccatgacagc ctgcaaatca tcacgagatt tgccagtcac
    19501 gcggatctca tcgccctgaa tttgcgcctg cactttcagt ttgctgtctt tgatcatctt
    19561 gacgattttt ttctgggtcg cgctctcaat gccctgcttc agtttcgctt ccacaaacca
    19621 ggttttaccg ctatgaacga tattttccgg tacatccagt gaactgcctt caatgccgcg
    19681 cttcagcagc ttggcacgca gaatatccag caactgattg acctggaaat cggactcgct
    19741 caacactttg atggttttgc tggcgtcgtt cagctcaaat gaggcttcaa cgttacggaa
    19801 gtcaaaacgg gactccactt cgcggctcgc gttatcgacc gcgttacgtg cttcctgaag
    19861 atcaacttca gagacaatat cgaaagatgg catcttttcc tctcccttca tttttgatgc
    19921 gaagcataat acccgcaaag ttaaataacc gacagtcctg caccaggacg tatcccttaa
    19981 ttgtgcatag tcgcaacagc atgcacaatt aagggatagc cttattgaac acccggtgtg
    20041 gttgcgggtg aggaggaaca atgaaaatta ccgtattggg atgcggtgcc ttagggcaat
    20101 tatggcttac agcactttgc aaacagggtc atgaagttca gggctggctg cgcgtaccgc
    20161 aaccttattg tagtgtgaat ctggttgaga cagatggttc gatatttaac gaatcgctga
    20221 ccgccaacga tcccgatttt ctcgccacca gcgatctgct cctggtgacg ctgaaagcat
    20281 ggcaggtttc cgatgccgtc aaaagcctcg cgtccacact gcctgtaacc acgccaatac
    20341 tgttaattca caacggcatg ggcaccatcg aagagttgca aaatattcag cagccattac
    20401 tgatgggcac caccacccat gccgcccgcc gcgacggcaa tgtcattatt catgtggcaa
    20461 acggtatcac gcatattggt ccggcacggc aacaggatgg cgattacagt tatctggcgg
    20521 atattttaca aaccgtgttg cctgacgttg cctggcataa caatattcgc gccgagctgt
    20581 ggcgcaagct ggcaatcaac tgcgtgatta atccactgac cgccatctgg aattgcccga
    20641 acggtgaatt acgtcatcat ccgcaagaaa ttatgcaagt atgcgaagaa gtcgcggcag
    20701 tgatagaacg cgaagggcat catacttcag cggaagattt gcgtcattac gtgatgcagg
    20761 tgattgatgc cacagcggaa aatatctcgt cgatgttgca ggatatccgc gcgctgcgcc
    20821 acaccgaaat cgactatatc aatggttttc tcttacgccg cgcccgcgcg catgggattg
    20881 ccgtaccgga aaacacccgc ctgtttgaaa tggtaaaaag aaaggagagt gaatatgagc
    20941 gcatcggcac tggtttgcct cgcccctggt agtgaagaga ctgaagccgt caccacgatc
    21001 gacctcttgg ttcgcggcgg tatcaaagtc accactgcca gcgtagccag cgatggcaac
    21061 ctggcaatta cctgctcgcg cggcgtgaag ctgctggcgg atgcgccgct ggtcgaggtg
    21121 gctgatggcg aatatgacgt gatcgtactg cctggtggca ttaaaggcgc agagtgtttt
    21181 cgcgatagca ctctgctggt tgaaaccgtt aaacagttcc accgttccgg gcgtatcgtc
    21241 gcggctattt gcgccgcgcc agccaccgtg ctggtgccgc acgatatctt cccgattggt
    21301 aatatgaccg gcttcccgac gctgaaagac aaaattcccg ctgaacaatg gcaggacaag
    21361 cgcgtcgtct gggatgcacg ggtaaaattg ctgaccagcc aggggccggg gacagctatc
    21421 gactttggtc tgaaaattat cgacctgctg gttgggcgcg agaaagccca tgaagtggca
    21481 tcacaactgg tgatggcggc agggatttat aattattacg agtagtgtcg gatgcggtaa
    21541 gcgtcgcatc tgaccagatg cgacgttaca accacaaatt acgggcgata taccttcaca
    21601 ttgttaaagc cctgctcgcg cagatagagc gcctgcagac ggctcatcac cccgcgctca
    21661 caccacagta gccaggttct gttctggtcg agatcgccaa acttggtgct cagtttatag
    21721 aacggcagag aaactacatc aatcccttcg actttcagtg gcttatcttc ctgttcatcg
    21781 atagaacgga tatcgaggat cacgtcgttc gggccgaagc cattgacggt ttccacttcc
    21841 accacttcct gctcggtctg ctgggcgatt tcgcggatat caacgttatt cgcttcttca
    21901 accactttat cgagaatgct gaagtcgaat ttctcttctt ccgcttcaat cttcgactta
    21961 accgctttca ccgtcgggct tttggagata acaccgcaat attccggcat cgtgcgagca
    22021 aagtcttcgg tgccaatctg gcgagccagg ttgatgatgt gctctttgtc gtaagaaatc
    22081 agcggacgca ggatcagcgt atcggagacg ttatcaatca ggcgcaggtt ggtcagcgtc
    22141 tgactggaca cctggccgag cgcttcgccg gtgaccagcg cctgtacgcc gtaacgttca
    22201 gccactttag acgcggcacg caccatcata cgtttgagga taacgcccat ctgaccgtcg
    22261 tcgattttct cgagaatttc cccgacgacc ggttcgaaat taatagcgac aaaacgcacg
    22321 cggtgggagc tgccaaaacg gttccacaga taatgcgcca cctgacgcac gccaatttca
    22381 tgcgccgcgc cgccaaggtt aaagaaacag taatgcacgc ggcagccgcg acgcatcaac
    22441 atataactgg aaacaccgga gtcgaaacca ccggaaatga gcgacagcac atcttcctgg
    22501 gtgccgatcg ggaaaccgcc aataccttcg tagcggcctt taatcagcag gagacgatcg
    22561 tcttccactt ccagatggac agtcacttcc ggattggtca gcttcacgcg cgcggactca
    22621 atatgctgat ttaaaccgcc gccgacgtaa cgttccacat caatcgagct aaaatcatgt
    22681 ttgccacggc gcttcacgcg tacgcagaag gttttgcctt ccagctgatc gcgatactga
    22741 accaacgctt tctcaaaaat atcgtgcatg tcggtaaatg gcacgtcttc gacttcgaga
    22801 atatggtgga tacccggaat acgggtcagg gcgtcgcgaa tagccagacg ctggttttca
    22861 tcttttgcgc gaacttcgat gttatcccag tggcggacga cagcgagcgt ctcatcatag
    22921 tgctttaaaa cgttacgaat gttcccggta aggattttta taaagcgcaa gcgcacagat
    22981 tggcttttga tggtgatttc cgggaacaat ttaatgataa acttcatggc ggcaatggtt
    23041 cgttggcaag ccttaagcga cttgtatagg gaaaaataca gcagcccaca cctgcggctg
    23101 catccaggcg cggaagtata ccactaacat cgctttgctg cgcacatcac cttaccattg
    23161 cgcgttattt gctatttgcc ctgagtccgt taccatgacg gggcgaaaaa tattgagagt
    23221 cagacattca ttatgccgaa gaaaaatgag gctcccgcca gctttgaaaa ggcgctgagc
    23281 gagctggaac agattgtaac ccgtctggaa agtggcgacc tgccgctgga agaggcgctg
    23341 aacgagttcg aacgcggcgt gcagctggca cgtcaggggc aggccaaatt acaacaagcc
    23401 gaacagcgcg tacaaattct gctgtctgac aatgaagacg cctctctaac cccttttaca
    23461 ccggacaatg agtaatggac tttccgcagc aactcgaagc ctgcgttaag caggccaacc
    23521 aggcgctgag ccgttttatc gccccactgc cctttcagaa cactcccgtg gtcgaaacca
    23581 tgcagtatgg cgcattatta ggtggtaagc gtctgcgacc cttcctggtt tatgccaccg
    23641 gtcatatgtt tggcgttagc acaaacacgc tggacgcacc cgctgccgcc gtagagtgta
    23701 tccacgctta ctcattaatt catgatgatt taccggcgat ggatgatgac gatctgcgtc
    23761 gcggtttgcc gacctgccat gtgaagtttg gcgaagcaaa cgcgattctc gctggcgacg
    23821 ctttacaaac gctggcgttc tcgattttaa gcgatgctga tatgccggaa gtgtcggatc
    23881 gcgacagaat ttcgatgatt tctgaactgg cgagcgccag cggtattgcc ggaatgtgcg
    23941 gtggtcaggc attagattta gacgcggaag gcaaacacgt acctctgaac gcgcttgagc
    24001 gtattcatcg tcataaaacc ggcgcattga ttcgcgccgc cgttcgcctt ggtgcattaa
    24061 gcgccggaga taaagggcgt cgtgctctgc cagtactcga caagtacgca gagagcatcg
    24121 gccttgcctt ccaggttcag gatgacatcc tggatgtggt aggagatact gcaacgttgg
    24181 gaaaacgcca gggtgccgac cagcaacttg gtaaaagtac ctaccctgca cttctgggtc
    24241 ttgagcaagc ccggaagaaa gcccgggatc tgatcgacga tgcccgtcag tcgctgaaac
    24301 aactggctga acagtcactc gatacctcgg cactggaagc gctagcggac tacatcatcc
    24361 agcgtaataa ataaacaata agtattaata ggcccctgat gagttttgat attgccaaat
    24421 acccgaccct ggcactggtc gactccaccc aggagttacg actgttgccg aaagagagtt
    24481 taccgaaact ctgcgacgaa ctgcgccgct atttactcga cagcgtgagc cgttccagcg
    24541 ggcacttcgc ctccgggctg ggcacggtcg aactgaccgt ggcgctgcac tatgtctaca
    24601 acaccccgtt tgaccaattg atttgggatg tggggcatca ggcttatccg cataaaattt
    24661 tgaccggacg ccgcgacaaa atcggcacca tccgtcagaa aggcggcctg cacccgttcc
    24721 cgtggcgcgg cgaaagcgaa tatgacgtat taagcgtcgg gcattcatca acctccatca
    24781 gtgccggaat tggtattgcg gttgctgccg agaaagaagg caaaaatcgc cgtaccgtct
    24841 gtgtaatcgg cgatggcgcg attaccgcag gcatggcgtt tgaagcgatg aatcacgcgg
    24901 gcgatatccg tcctgatatg ctggtggtcc tcaacgacaa cgaaatgtcg atttccgaaa
    24961 atgtcggcgc gctcaacaac catctggcgc agctgctttc cggtaagctt tactcttcac
    25021 tgcgcgaagg cgggaaaaaa gttttctctg gcgtgccacc cattaaagag ctgctcaaac
    25081 gtaccgaaga acatattaaa ggcatggtag tccctggcac gttgtttgaa gagctgggct
    25141 ttaactacat cggcccggtt gacggtcacg atgtgctggg gcttatcacc acgctgaaga
    25201 acatgcgtga cctgaaaggc ccgcagttcc tgcatatcat gaccaaaaaa ggtcgtggtt
    25261 atgaaccggc agaaaaagac ccaatcactt tccacgccgt gcctaaattt gatccctcca
    25321 gcggttgttt gccgaaaagt agcggcggtt taccgagcta ttcaaaaatc tttggcgact
    25381 ggttgtgcga aacggcggcg aaagataaca agctgatggc gattactccg gcgatgcgtg
    25441 aaggttccgg catggtcgag ttttctcgta aattcccgga tcgctacttc gacgtggcaa
    25501 ttgccgagca acacgcagtg acctttgccg ccggtctggc gattggtggg tacaaaccca
    25561 ttgtcgcgat ctactccact ttcctgcaac gcgcctatga tcaggtgctg catgacgtgg
    25621 caattcaaaa gcttccggtc ctgttcgcca tcgaccgcgc gggcattgtt ggtgctgacg
    25681 gtcaaaccca tcagggtgct tttgatctct cttacctgcg ctgcataccg gaaatggtca
    25741 ttatgacccc gagcgatgaa aacgaatgtc gccagatgct ctataccggc tatcactata
    25801 acgatggccc gtcagcggtg cgctacccgc gtggcaacgc ggtcggcgtg gaactgacgc
    25861 cgctggaaaa actaccaatt ggcaaaggca ttgtgaagcg tcgtggcgag aaactggcga
    25921 tccttaactt tggtacgctg atgccagaag cggcgaaagt cgccgaatcg ctgaacgcca
    25981 cgctggtcga tatgcgtttt gtgaaaccgc ttgatgatgc gttaattctg gaaatggccg
    26041 ccagtcatga agcactggtt accgtagaag aaaacgccat tatgggcggt gcaggcagcg
    26101 gagtgaacga agtgctaatg gcccatcgta aaccagtccc cgtgctgaac attggcctgc
    26161 ctgacttctt tattccgcaa ggaacgcagg aagaaatgcg cgccgaactc ggcctcgatg
    26221 ccgccggtat ggaagccaaa atcaaggcct ggctggcata atccctactc cactcctgct
    26281 atgcttaaga aattattcat ggcaggagtg gcagcatgca atacaacccc ttaggaaaaa
    26341 ccgaccttcg cgtttcccga ctttgcctcg gctgtatgac ctttggcgag ccagatcgtg
    26401 gtaatcacgc atggacactg ccggaagaga gcagccgtcc cataattaaa cgcgcactgg
    26461 aaggcggcat aaatttcttt gataccgcca atagctattc cgatggtagc agcgaagaga
    26521 tcgttggtcg cgcactgcgg gatttcgccc gtcgtgaaga cgtggtcgtt gccaccaaag
    26581 tgttccatcg cgttggtgat ttaccggaag gattatcccg tgcacaaatt ttgcgctcta
    26641 tcgacgacag cctgcgacgt ctcggcatgg attatgtcga tatcctgcaa attcatcgct
    26701 gggattacaa cacgccgatc gaagagacgc tggaagccct gaacgacgtg gtaaaagccg
    26761 ggaaagcgcg ttatatcggc gcgtcatcaa tgcacgcttc gcagtttgct caggcactgg
    26821 aactccaaaa acagcacggc tgggcgcagt ttgtcagtat gcaggatcac tacaatctga
    26881 tttatcgcga agaagagcgc gagatgctgc cactgtgtta tcaggagggc gtggcggtga
    26941 ttccggcgcg ggggcgactg acgcgtccgt ggggagaaac taccgcacga ctggtgtctg
    27001 atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa tgacgcgcag atcgcagagc
    27061 ggttaacggg cgtcagtgaa gaactcggtg caacacgagc acaagttgcg ctggcttggt
    27121 tgttgagtaa accgggcatt gccgcaccga ttatcggtac atcgcgggaa gaacagcttg
    27181 atgagctatt gaacgcggtg gatatcactt tgaagccgga acagattgcc gaactggaaa
    27241 cgccgtataa accgcatccg gtagtaggat ttaaataaga gtccaggcct gatgagacgc
    27301 aacaagcgtc acatcaggca tcggtgcaca actacgacag aatgcccagc ggccagtgat
    27361 gaccaataaa gtacaggatg ccagcggaga tcaccccggc gacaatatca tctatcatga
    27421 tccccatgcc gccatgcaca ttgcgatcaa accagcggat cggccacggt ttccacatat
    27481 ccagaatacg gaaaatcaca aacccggcgg caacccactg ccagtcattg gtcggcagcg
    27541 ccatgagcgt gatccacata ccaataaatt cgtcccagac aatgctgccg tgatcgtgca
    27601 cgcccatatc ttttgcggtc tgatgacaaa gatagacgcc gatacagatc cccagcatca
    27661 ccaccagcga gtagagctgc cagggcaaaa aggtcatcag ataccagaac ggaatcgctg
    27721 ccagcgagcc catcgtccca ggaacgatcg ggcttaatcc acttccgaat ccgacagcaa
    27781 gtagatgcca cggattactc atcttcaggc gacttttcgc gacatcttta tggcgtggca
    27841 aaatggtcat atcctttcca gtctaacgta acaggttcac cgtcacgaat aaaacaaagc
    27901 ccttcgatat cggcggtcat ttgcccgata caggtaaacg gtacgcccag gtgtccgaga
    27961 gccacatcca gcgctccacg gttcagttcc ggcaccgtga aacacaactc gtaatcttca
    28021 ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa catggcgaga aagcgcatca
    28081 gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt cgctggcttt cacgatatgc
    28141 ccgagatcgg aaatcagacc gtcagagaga tcgatagctg aatttgccag atcgcgcagt
    28201 gcctgcccct gtaaaatacg cggcgatgga cggagatgac gtttgatcaa gtagtccgca
    28261 tctttagcat cggcaacctg caaacggttt tgcaaaatcg ccagcccggc ggcgctatcg
    28321 cccggtgtac cggtcacata gatccagtca cccggtttcg ccccaaagcg cgttaaggct
    28381 cgtcccatcg gaacaaagcc gtggataccc aacgtcattg ataatggccc acgcgtggta
    28441 tcgccgccaa tgagttgcat atcgtaataa ttgagaagat caaacaaact gtcgctgaag
    28501 gactcaagcc acgcttcgtc tacgtccggc aaggttaatg ccagcgtcag ccaggccgga
    28561 tcggccccca tcgctgccag atcgcttagg ttcaccgcca gtgctttata cgccagatca
    28621 gcaggatcga tatcagggag gaagtggttg cccgccacca gcgtatcagt gctgatcgcc
    28681 agggtctgtt tctcggggat attgagaagt gcgcaatcgt cgccgatgcc cagttcgaca
    28741 tcaagacgag aacttcttac acggtcaaaa taacgggcaa tcagggagaa ctcgccacat
    28801 gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg gcgatcatgg aacggtcttc
    28861 cgtgaatcta ccggcctgga tattactttt tgttagggcg aatcacaggt gctgctttat
    28921 cgagtacacc gttgacgaac ttatggctgt cttctgcgcc gaacgatttc gccagttcga
    28981 tcgcttcgtt aatggccact ttgtatggca catcgctacg tttagacagt tcgtacagcg
    29041 caatgcgcag tactgctttt tctacctgtc ccagttcttc cagcagacgg gacaggtatg
    29101 gcttcatcag tccgtcgagg tatgcggtat tagtcgccac cccggccagc agctcacgga
    29161 agtacaggac gtcaacgtct tttacatcct gttcagccag gaactggtat tcaacatcag
    29221 cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg gacggcacac tcacgagcgc
    29281 ggcgacgagc agcaggtttc acggatttcc ccttactaat ttcaggcctt gatggctttc
    29341 aatacattaa tcatttcaag cgcggtcagt gcagcttctg cacctttgtt gccagctttg
    29401 gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg tcagaacccc aaaagcaacc
    29461 ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc ttgcaccacc agcgacatat
    29521 tcaaaatggg cagtgccacc acgaataacc gtacccagcg caatcaccgc gtcgtattta
    29581 ccggttttag ccagtgcacc cgccgccagc ggcagctcat aggcaccagg cacccaaaca
    29641 acggtaatgt tttcatcttt tacctgaccg atacgtttca gtgcgtcaat tgcaccttcc
    29701 agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg tgatggcgac gcgagcgtcc
    29761 ggggtagcaa cgttagcttc aataatgttc attttcttcc ttcgggttcg agtatggccc
    29821 cgcagggggg cggattttag cataatattt cgtgcgctgc ttccctttcg agccgggaga
    29881 tcatgcaccc actaaatgca ggcaaacatc cgggcctaca tgacgtatct ctttgaattt
    29941 aaattggggg gcgtcggcta atttctcaag ccctggcagg gtgcataatc cacgggcgtc
    30001 gctgcctaat agtttaggtg cgatatagac aatcagctca tcgactaaac ccgcctgcag
    30061 caatgcgcca gcgagcgttg gccccgcatc cacccagatg ctgttaatct gctgtttacc
    30121 cagttgcatc atcagtacaa ccagatccag atgaccttta tgctctggaa tcagcaaggt
    30181 acgcaccgtt tccggccact cacgagaatc ttcctgcgta cgcgcgaacc aggtttcgcc
    30241 gggctgctgc acaatgtgat gttccggcgt cacgcgattt tggctatcaa tcacaatacg
    30301 taccggctgg cggagatttt gttgcgggta gagcgcctga gtttgttcat ccagttcgga
    30361 ccaacgcacc gttaaggcag gatcatccgc cagcaccgtg gcgctgctgg ttaaaatggc
    30421 atgactttgc gcgcgcagtc gttgtacatc acgccgcgcc tggggcgaag tgatccactg
    30481 gctttcgccg ctcgccatcg ccgtgcgacc atcaagcgat gcgccaagtt ttaactgaat
    30541 ataaggaaag ccggtgcgca tccgcttgag aaagccttta ttcaattgct cggcttcact
    30601 catcatcagg ccgtggctga cgtcaatgcc agcctgttgc agacggtaaa gtccacgccc
    30661 agcgacctgc gggttaggat cttgcatcga ggcaaccacg cgcgccacac ctgcggcaat
    30721 taaagcatca cagcaaggcg gcgtacggcc gtgatggctg caaggttcga gtgtcacata
    30781 cgccgttgca cctttggctt tttcacccgc catacgcaac gcgtgtactt cggcatgtgg
    30841 ttcacccgct cgttgatggt aaccttcacc gacaatttcg ccatctttga caatgacgca
    30901 cccgacattt gggttggggt gggtggtaaa acgtcctcgt tgcgccagct ttagcgcccg
    30961 cgccatgtaa tactcgtcct gcacggctta gtcctccagg cgcgcgatct cttcgccaaa
    31021 ttctttgata tcttcgaaac tgcggtagac agaggcaaaa cggatatagg cgactttatc
    31081 gagctttttc aattgctcca tcaccagatt gccaatcatc ttgctcggta cttcgcgctc
    31141 accggtggcg cgcagctgcg atttaatatg attgattgcc atttcaacgt catcggaact
    31201 caccggacgt ttttccagcg cccgcagcat tccgctacgc aatttctctt cattaaacgg
    31261 ttcacgcacg tcgttgcttt ttacaacacg cggcataacc agctccgcca cttcaaaggt
    31321 ggtgaaacgt tcattacaca ccagacactg ccgacggcgg cgtacggatg aaccctcgcc
    31381 cacgagacga gagtcaatta ccttagtgtc cacggcgaaa cagaatgggc aatgcatacg
    31441 gcgtcctgac caggtggtta acggaaatct attttaccct gaactgacga tacaacaaag
    31501 gcgcaacgct tttgagataa aggatcgatg ctttcgctgc ttttaccctc taccattagg
    31561 ctaattgcga cgagaaaggg acgacctatg aatactaacg tttttcgact gctcctgctg
    31621 ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg aagtgcgaca gatgaaacac
    31681 agcgtcagca cgctgaacca ggagatgacg caactcaatc aagaaacagt caaaatcact
    31741 cagcaaaaca ggctgaatgc aaaatccagc agtgggggtt accttctgcc tggagcgaaa
    31801 acaccggcaa gactggaaag ccagatcggt actttacgta tgtcgctggt gaatattacg
    31861 cctgatacag atggcaccac ccttacacta cgtattcagg gtgagtcgaa tgatcccttg
    31921 cccgcattca gcggaactgt tgaatatggg caaattcagg gaacaataga caactttcag
    31981 gaaatcaatg tgcagaatca attgattaat gcccctgcca gcgtcctcgc ccccagcgat
    32041 gttgatattc cgttacaatt aaaggggatt tctgtggatc agttaggctt tgtacgtatc
    32101 cacgacattc aacctgttat gcagtaaacg tatttcggga cgattttgtg cgtcccgcaa
    32161 catctttccc cgtcattttg ttactctgct tacatcacct agattgatag taaaagtttg
    32221 caacaagggc gaaagtcagt acaatccccg ccctaatgtg tgtaaacgtg aacgcaatcg
    32281 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    32341 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    32401 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgtggtact
    32461 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    32521 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    32581 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    32641 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    32701 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    32761 caatactgat cttaacttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    32821 cgacatgggt cgtaataaaa atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    32881 catcgatact ggtctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    32941 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    33001 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    33061 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    33121 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    33181 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    33241 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tcgtaggtta
    33301 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    33361 gagctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    33421 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    33481 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    33541 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    33601 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga caaagcaatt cccagttact
    33661 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    33721 gttggaataa acaaactcgc gggaacagcg accgcagacc cacggatata tttttaatgc
    33781 tttttcgcga tagccgcttt ctaaccgcgc atagtttttt gggatgatag ccatgaagag
    33841 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    33901 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    33961 gattacggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    34021 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ccacatagat ggaagatgca
    34081 gtaccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    34141 aagaggtaca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    34201 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    34261 aagttttcac gaatacggtc cgataccacg atactgtcgt taagcgagta accgataacc
    34321 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    34381 agcgtaatga taacgtcgtg cgccagcgca ataacaaccc ctgccgccag tcgccactca
    34441 aagcgaaaac ctacgtacac aaggatagac agcagcgccg ccatcaacgc catcgcaccg
    34501 gtttgcgcaa ggtctgcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    34561 ttctgattgg tggattcgtt aatcaccttc agaacctggc tgcccagcac ctgaccgccg
    34621 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    34681 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    34741 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    34801 ccgcgcacgc ccataataac gatagcggcg attaacagca gaccagagat gccgaaagcc
    34861 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    34921 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    34981 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    35041 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    35101 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    35161 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    35221 gatcagtacg ttcgcatcga ccgccaccgc aagggttaag acgatacccg cgatacctgg
    35281 catactcagc gttgcgcctg gcaacagcga cataatgccg acgattaaga tcaagttggc
    35341 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    35401 agaaaccagc agaccggcaa ggcaagcctc cagcccctgt tcaatgttct gcatacccag
    35461 ggttgggcca atggtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    35521 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    35581 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    35641 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    35701 cggtttgccg atgttgtcct tagtgaagtt agacatgatg ttaccaccag cgctatcgag
    35761 cgagatgtta acctgcggtt ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    35821 gtcaccggtc agaattacgc gtttgtacag cacaactgac tgaccttcgc gggtctgttt
    35881 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctggtcaa cgttggtgtt
    35941 taccagacgg aattccagcg ttgccgtcgc acccagaatc tctttcgcac gcgcagtgtc
    36001 ctgaatacct ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    36061 cgctacgcca agttggttta cacggttacg caggatattg atgttctgct gcaccgcata
    36121 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    36181 gctactaatc actaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    36241 tttagcatcg cggaaagtaa tgctcaggcc gtagttgttt tctttacgaa cggtggtata
    36301 cgggatgccc ttttcacgca gatcgctgcg caggctatcg atattttgtt cctggagttt
    36361 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    36421 gccgagcttc atcggctcag cgtgaatagc tgccagccag cgcggcgttg ccggggcaag
    36481 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcag
    36541 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    36601 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    36661 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    36721 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    36781 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    36841 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    36901 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    36961 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    37021 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    37081 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    37141 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    37201 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatgg
    37261 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    37321 gcgcgcctaa tatttcgttg caacggtcaa gatgatgcaa gtaagcgcgt gaataattgc
    37381 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tacttcgcat
    37441 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    37501 gcattacgca gtcaaacata tcgataccac gacgtacgcc ttcaaccagg tcttctggtt
    37561 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcgggcat acatgctcca
    37621 gaatgcggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    37681 caaaaccgat atctaccaga cctttaacag aaatatcacg taaatcttcg taaacgctgc
    37741 cctgaatgat accaaacagc gcatttttgt ttccgagact gtcaaaacgc tcacggctac
    37801 gcttcgccca acgcagagac atatccatgg agcgttttgc gtaatcccag tcagcaggat
    37861 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    37921 ttgatttttc aggatcgagg aaaatcggat cgccgttgat cgggttacgg aagtgcacgc
    37981 cctgttcggt gattttacga atatcgccaa ggctgaagac ctggaagccg ccggagtcgg
    38041 tgaggatcgg ccccttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    38101 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    38161 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    38221 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    38281 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgtttt
    38341 aaaccagcgc cgcggaatta ctccccgacg cgctcattaa ttgcctgcgg attgtacgta
    38401 ataaacatcg catcaccgta actaaaaaag cgatatttct cttctaccgc tgctttatag
    38461 gcgttcatgg tgtgttgata accggcaaag gccgaaacca gcataatcag cgtcgactct
    38521 ggcaagtgga agttcgtcac cagcgcatcg accactttgt actggaagcc cggatagata
    38581 aagatttggg tatcgtcgaa gaacggttca atgagatcgt ttttcgctgc ctgagccgcg
    38641 ctttccagcg aacgtactga agtggtgcca accgcaatca cccggttacc gcgcgctttc
    38701 gccgccagta ccgcgtctac cacatcctgc ggtacttcag cgtattccga gtgcatgatg
    38761 tgatcttcaa tggtgtcgac gcgcaccggc tggaaggtgc ccgcaccaac gtgcaacgtc
    38821 acaaacgcca tctccacgcc tttggcgcgc aatttttcca gcaaaggctc gtcaaaatgc
    38881 agaccagcgg tcggggctgc aaccgcgccc ggtttttcgc tataaacggt ttgataaagt
    38941 tcgcggtcag cgtcttcgtc cggacggtcg atatacggcg gcagcggcat atggccgata
    39001 ctgttgagga tatccagtac cgaacgatca tcattgaatt cgacttcaaa cagtgcgccg
    39061 tggcgcgcgg tcattgttgc gttaatactt tcgtcatcgc ccagcagcag ttctgcgcca
    39121 ggttttggcg ctttcgaggc gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgc
    39181 tcaaccagca cttcaatctt gccgccgctg gctttacgcc caaacaggcg cgccgggatc
    39241 acgcgagtat tattaaaaac cagaagatcg ccggggttga gcttatcgag taaatcggtg
    39301 aaagtaccgt gcgtcagcgc gcccgtaggc ccgtccagcg acagtaaacg acagctactg
    39361 cgttcaggca tggggtagtg ggcaatcagg gattcgggca attcaaagga gaaatcggta
    39421 acgcgcatga cactgactca gactaaaata agaggcgggt agtctagtgc cggggcgctc
    39481 tccctgcaac ctttaccccc ctttggatag aatataatcg atgaattttt tagctcacct
    39541 gcatttagcc catctcgcgg aaagctcgct ttccggcaat ttactggctg atttcgtacg
    39601 cggaaatcct gaagaaagtt ttccgcccga cgtcgtggct ggcattcata tgcatcgacg
    39661 tatcgacgta ttgactgaca atctgccgga agtccgcgaa gcacgggagt ggtttcgtcg
    39721 tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact ttctttcccg
    39781 ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttatctgtt atgcccgcaa
    39841 gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctaa acaattactt
    39901 gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga acgtgttaaa
    39961 cggcatggca agccgtcgcc cacgtctgga tgccctgcgt gactcctggt acgatttaaa
    40021 cgcccattat accgccctcg aaacccgctt ctggcagttt tatccgcgga tgatggcgca
    40081 ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga aatggcaggc
    40141 aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca ttgcacggca
    40201 ttgagaaacg gtgacgcggg tagcaccact tcacaggcct cgccacggtt gattgccacc
    40261 agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc cgcatacagc
    40321 acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat cattcgccgg
    40381 tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg tttacggcaa
    40441 aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca cggtacacca
    40501 ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg accgagcagc
    40561 gttttaaatc gcgcagtatc gtggctgtcg agctgattaa acatacgtaa ttgttgttga
    40621 tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc atcaatttgc
    40681 tgcggatcgt aagagatatc ggtattggca agaaatcccc acaacgggaa tgtgaagcca
    40741 cgatagttca tggcggcatc ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa
    40801 tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt gatcccagca
    40861 acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac cacatccagc
    40921 cgccagccgt ccatactcca cggcgctttc agccaatggc ggacaatact gtcttccccg
    40981 cgataaattt cattcaccag actttccgac tgataatcca gcttcggcaa gctggcatag
    41041 ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg ccagggcgat
    41101 tcagggttgt gacaagctcc ccccgtgcca cgattgtgcc tgtcaaacca ggcatgggaa
    41161 tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg ctgcgtattg
    41221 tgtcgcaaaa gcagcaacgc cccatcaccg ccaaactgcg gatcgacatg gcgataatcc
    41281 tcggtatcgt attgatgtac gctgggagct ttaaacaccg gattgagata cagcgctgtc
    41341 acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc gccgccatag
    41401 aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa gatgatctct
    41461 tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg aagactacgc
    41521 gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg tgggccgata
    41581 tccggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct gaagccctgc
    41641 ggtgacgcct ggctgcggct gactgcgctg cttatgcatt ggtactgaca tttcttcgtt
    41701 atcgtgttct gtacgcagca taatgcgctg cggtgggtct tcgcccgtca gccacagtgt
    41761 aatgagcagt tgatctttgc tttgtttaac aaatgggggc accggcaggt gccatgcatt
    41821 taacatcata attcccctgt gatgaaaatc tgatcacctt gccatagcct aatgcaggat
    41881 cactcatcat ctgaaggata tttgggggag gaggacgggg gcggataact ttcggaatag
    41941 ccggatcagg atttcctcct gaaccggctt acagaacgca aggattactg gttttcagcc
    42001 agctgacgat cgtggcgacg cttaaacatc cagccaatca acagcacaac aatccacgcg
    42061 aaaccgacat acagcgagat acgcgtatcc gggtgataac caatcaaccc gataataaag
    42121 agcaggaaaa tcagcccgcc gatggtcgtt gctaccccac ccggcacttt aaatttcagc
    42181 gccttaactt cttctggcgg caaacggcga cggaaggcga tttgcgacag caggatcata
    42241 atccacaccc ataccgtggc gaaggttgcc agcgaagcga tcaccaggaa gacgttttcc
    42301 ggcatgatgt agttcagata caccgcaaac agcagcgcgg tagtcatcac cagcaccgta
    42361 acccacggaa taccgcgacg cgacgttttg ctgaaaattt tcggtgcgct gccctgctct
    42421 gccataccgt ggagcatacg gcctacgcca aatacgtcac tgttaattgc cgacagcgaa
    42481 gcggtcagca caacaaagtt aagaatgctg gcggcaaagg taatgcccat atgctggaac
    42541 gtcagcacga acgggctacc ggcagtgcca acctgattcc acgggtagat agacataatg
    42601 acgaacagcg taccgacgta gaacaccaga atacgcatcg gcacggagtt aatcgcacgc
    42661 ggaatcgatt tctcaggatc tttcgcttca ccggcggtaa tcccgataat ttcgatccca
    42721 ccgtaagcaa acatcaccat ttgcaacgac attaccatgc caagccagcc gttactgaag
    42781 aagccgccgt tgctccacag gttatgaata ccggtcggtt gcccgccgtt gccaatcccc
    42841 cagatgatga tgccgaaacc ggcgacaatc atgatgatga tggtggcgac tttaaagaac
    42901 gagaaccaga attccagctc accgaatacc ttcacgctca tcaggtttac ggcgcaaatg
    42961 atcagcacca cgctcagtac ccaaatccag tgcggcaccg tcgggaacca gacacccata
    43021 tagataccaa aagcggtcac atcggcgatg gcgacaatta ggatttcaaa gcagtaggtc
    43081 cagccggtaa tgtaacctgc cagcgggccg aggttttcct gcgcataacg cgagaaagag
    43141 ctggcggccg ggttatgtac cgacatttcc cccagcgcac gcataatgat atacgccgcg
    43201 ataccaccga taatataggc caacaacacg ctcggaccgg ccattttgat ggcgtctgcc
    43261 gaaccgtaaa acagcccggt gccaattgct gaacccagtg ccataaagcg tatgtggcgg
    43321 gtacttagcc cacgctttag cttgttctta ctttccatca attcctaacc catcaacaca
    43381 ataaaaaacc acggggcatc gcagccccgt ggttaaaaca aatgttcagt gatttagtga
    43441 gcgctggagg tcacctgacg acctgccgcg cgatcccaga taatggccag aaccaccatc
    43501 accactgttg gcattaacca cgccaggcct tgttcggcca gcggtaaacg ctgcgcccag
    43561 gacggtaaga tatcgctgaa tgcagatgct ttgatcccgt cgagaatacc aaaaagcagg
    43621 ctgataaaca tcggcggggc aatcacgcgg gacgaattat gccaccatga gcgtgtaaaa
    43681 cttaatacaa ccagtgcgat acacggcgga taaatagcgg tcagcaccgg tacggagatc
    43741 tggatcagct ggcttaagcc gaggttagac accaccatcg agaagccgcc gaggataaac
    43801 accagcgtac gataagagag cggtacgtac tgggcaaaga attctgcaca agcacaggtc
    43861 aggccaactg ccgttaccag gcaggcgatg aagattaacg ccgccagcag gaagctaccg
    43921 ccgccgccaa atgtgtgctg aacgtaagca tgcagaatag cagcgccgtt tgcagactga
    43981 tcgaccagcg acgcgctgtc tgaacccaga cggaacagcg ccaggtacag cagagtcaga
    44041 ccaacacccg ccatcaggcc agcccagacg gtataacggg tcagcagacg cgcttcggta
    44101 acgccacgag aacgcgccgc gttaacaata acgataccaa acaccattgc gcccagcgta
    44161 tccatggtca gatagccgtt aacgaagccg ttagaaaacg cagcgttttg ataagcctca
    44221 gtcgccgtgc tgatagaacc cgccggccag acaatagcgg caacagacag gatgaccagc
    44281 gcgataattt tcagcggcgc aaggaagttg cccacggtat ccagcagctt gcccggatag
    44341 agcgaaacca gaataacgat agcgaaatag accaggctgt agataaacag cggcagcgcg
    44401 gaatcacccg tcagcggcgt aatccccact tcaaaggaaa cggtagctgt acgcggcgta
    44461 gcgaaaagcg gccccaccgc caagtaacaa accgttgcca gcagcacgcc agcgacttta
    44521 ccgattggcg tgctgaggct gtcaacaccg ccgccaactt ttgccagcgc cactaccgtt
    44581 aataccggca ggccaacggc agtaatgagg aagccgaatg ccgcagtcca gacgtgttcg
    44641 cctgcctgca agccgaccat tggagggaaa ataatgttac ctgcgccgac gaacaacgca
    44701 aatgtcataa agcccagagc gatgatatcg cgcgatctta attgatgggt cataaatcat
    44761 actgcctgtg gatgtggtgt tgtaacgtta aatttttgtg ccaatccctg gggatgatac
    44821 agcggtatat ttaaacaatt ttagcgggaa atactcccaa ccaggcgcgg cgaagaatag
    44881 tttttcgatt taccgcgtta atgcagtcag tatgacagca cctgaagcgc aatttaaacg
    44941 cttataacgt tttaaagcaa ggagggatct aaaaaccaga ggtatatagc gataagaaat
    45001 caccaaccag tataaaacac tatttccgtg aaaaagacat ggctaatcac gcgaacaaaa
    45061 aatcaccaac cgttgtaaaa tctaacgccc gtattttgac tgcaaagaaa ataagccagc
    45121 atcgactggc ttatggcaat aaaagatgac aaaggcggat taatcgctgt ttttggcaat
    45181 taaacgttcc gggataacaa aactgaaacg tgttcctttt cctactgtac tctcaatatt
    45241 caggcgactt tcgtgatgat tcacagcatg tttcacgatc gctaacccta atccgctacc
    45301 gccggtttgc cgggaacgcg ctttatcaac gcgataaaaa cgctcggtca ggcgcggaat
    45361 atgctccggt gcaatgcccg gtccgttatc ttcaacgcta aattcggcac cgtgcggcac
    45421 tcgctgccag cgtacggtga tatgcgtgcc ttctggagta tgattcacgg cgttatagac
    45481 caggttcgaa atcgcactgc gtagctgatc ttcgttgcca gacactttga ggccgttatc
    45541 tatctcaaag gtaaatgtct gttttttctg actcagagtc tgagcctcgc gctcaacaac
    45601 gcgcagcatc atcggcacat caaccttttc attgagcaat tgcgtcggcg cggcttcaat
    45661 tttcgacagc gttagcaact gcttcaccag cccttccatc cgctgggtct gctcgcgcat
    45721 ggtgtgcaac gctttttcgc gtaccgcgcc ttccagcggc tgctcatcca tcatctccag
    45781 gtaaccctgt aacacggtca atggcgtacg taactcatgg ctcacgttgg caaaaaagtt
    45841 acgccgcgcc ccttccagtt gatgcatttg cgtgacatca cgcgccacca ttagcaactg
    45901 tttgtgggta taaggcatga cgcgaatttc cagatgccgc ccggtgttga gcaccagatt
    45961 gagcgggcga gaaaaatcac gcgttttcag atattgcgta aactccgggt aacgcagtag
    46021 gttaaggatg ttctgcccgt tatcttccgg ccagcgcaaa ccaagaattt gttgcgctag
    46081 accgttacac cagaaaatac cgccctcttc cgtggtcagc accaccgcgt cgggcagcga
    46141 ctccgcgccg ctacgaaagc gtttaatcag attgcccagt tcacggcggc gttttttatt
    46201 tcgcagctgc atctggtgta agccgtatag tagcggttcc cagctaccac gccccggtgg
    46261 cggggtcata atgcgatcca cccacaacca ccatgaaagg cgcaataaat tccagaaatg
    46321 ccagataagc agtcctgtta ccgatgccag caaaaaccag ggcaggtaac caaaaaatgc
    46381 acccaggatg aaagccggga ggcagcaaag tagcagctcc agcaccagcc ttttccacga
    46441 cagccgttcc agcaggtaag atactccagt taagaaatca taagccctgc tctgcgtccg
    46501 atgagcaagg cgttaaaagc gggttgaaaa acgatatcct gtaccgcgca cggtctgcac
    46561 catgcggtca tgcccgccgg gctccagtgc tttacgcagg cgacgaatgt ggacatcgac
    46621 cgtgcggtct tccacataca cgttagttcc ccagacgtgg tttaacagct gctcgcggct
    46681 gtacacgcgc tcaggatgcg tcataaaaaa gtgcagcagt ttaaattctg tcggccccat
    46741 ctccagcggc tcttcgcccg ccatcactcg gtgagatgtc gggtcgagac ttaatccctg
    46801 catctcaatc acctcttcca ccgccattgg cgaaatacgg cgcattaccg ctttgattcg
    46861 cgccaccagc tccttcggcg aaaacggctt ggtgatatag tcatccgcgc cggtttcaag
    46921 gccgcgcacg cgatcttctt cttcccctct ggcggtcaac atcaccactg gaatatcccg
    46981 ggtcatcgac tcgcgcttga ggtgtttgat gaactggata ccggagccgc caggtaacat
    47041 ccagtcaagg agaattaaat ccggccaggg ttcattcagt tgattcacag cactgtcata
    47101 atcttccgct tcgaccggct gaaagccatt ttgttcgagc acgaagcaga ccatttcgcg
    47161 aattggagct tcatcttcta cgaccagaat acgtctcgcc atgatttgcc ctgttgtaat
    47221 aaataggttg cgatcattaa tgcgacgtca ttatgcgtca gatttatgac agatttatga
    47281 aaagctcgtc gcacatatct tcaggttatt gatttccgtg gcgcagaaaa aagcaaatgg
    47341 cacatctgtt tgggtataat cgcgaccatg ctttttcgcc agggaaccgt tatgcgcatc
    47401 cttcacacct cagactggca tctcggccag aacttctaca gtaaaagccg cgaagctgaa
    47461 catcaggctt ttcttgactg gctgctggag acagcacaaa cccatcaggt ggatgcgatt
    47521 attgttgctg gtgatgtttt cgataccggc tcgccgccca gttacgcccg cacgttatac
    47581 aaccgttttg ttgtcaattt acagcaaact ggctgtcatc tggtggtact ggcaggaaac
    47641 catgactcgg tcgccacgct gaatgaatcg cgcgatatca tggcgttcct caatacaacc
    47701 gtggtcgcca gcgccggaca tgcgccgcaa atcttgcctc gtcgcgacgg gacgccaggc
    47761 gcagtgctgt gccccattcc gtttttacgc ccgcgtgaca ttatttccag ccaggcgggg
    47821 cttaacggta ttgaaaaaca gcagcattta ctggcagcga ttaccgatta ttaccaacaa
    47881 cactatgccg atgcctgcaa actgcgcggc gatcagcctc tgcccatcat cgccacggga
    47941 catttaacga ccgtgggtgc cagtaaaagt gacgccgtgc gtgacattta tattggcacg
    48001 ctggacgcgt ttccggcaca aaactttcca ccagccgact acatcgcgct cgggcatatt
    48061 caccgcgcac agattattgg cggcatggaa catgttcgct attgcggttc ccccattcca
    48121 ctgagttttg atgaatgcgg taagagtaaa tatgtccatc tggtgacatt ttcaaacggc
    48181 aaattagaga gcgtggaaaa cctgaacgta ccggtaactc aacccatggc ggtgctgaaa
    48241 ggcgatctgg cgtcgattac cgcacagctg gaacagtggc gcgatgtatc gcaggagcca
    48301 cctgtctggc tggatatcga aatcactact gatgagtatc tgcatgatat tcagcgcaaa
    48361 atccaggcat taaccgaatc attgcctgtc gaagtattgc tggtacgtcg gagtcgtgaa
    48421 cagcgcgagc gtgtgttagc cagccaacag cgtgaaaccc tcagcgaact cagcgtcgaa
    48481 gaggtgttca atcgccgtct ggcactggaa gaactggatg aatcgcagca gcaacgtctg
    48541 cagcatcttt tcaccacgac gttgcatacc ctcgccggag aacacgaagc atgaaaattc
    48601 tcagcctgcg cctgaaaaac ctgaactcat taaaaggcga atggaagatt gatttcaccc
    48661 gcgagccgtt cgccagcaac gggctgtttg ccattaccgg cccaaccggt gcggggaaaa
    48721 ccaccctgct ggacgccatt tgtctggcgc tgtatcacga aactccgcgt ctctctaacg
    48781 tttcacaatc gcaaaatgat ctcatgaccc gcgataccgc cgaatgtctg gcggaggtgg
    48841 agtttgaagt gaaaggtgaa gcgtaccgtg cattctggag ccagaatcgg gcgcgtaatc
    48901 aacccgacgg taatttgcag gtgccacgcg tagagctggc gcgctgcgcc gacggcaaaa
    48961 ttctcgccga caaagtgaaa gataagctgg aactgacagc gacgttaacc gggctggatt
    49021 acgggcgctt cacccgttcg atgctgcttt cgcaggggca atttgctgcc ttcctgaatg
    49081 ccaaacccaa agaacgcgcg gaattgctcg aggagttaac cggcactgaa atctacgggc
    49141 aaatctcggc gatggttttt gagcagcaca aatcggcccg cacagagctg gagaagctgc
    49201 aagcgcaggc cagcggcgtc gcgttgctca cgccggaaca agtgcaatcg ctgacagcga
    49261 gtttgcaggt acttactgac gaagaaaaac agttacttac cgcgcagcag caagaacaac
    49321 aatcactaaa ctggttaacg cgtctggacg aattgcagca agaagccagc cgccgtcagc
    49381 aggccttgca acaggcgtta gccgaagaag aaaaagcgca acctcaactg gcggcgctca
    49441 gtctggcaca accggcacga aatcttcgtc cgcactggga acgcatcgca gaacacagcg
    49501 cggcgctggc gcatactcgc cagcagattg aagaagtaaa tactcgctta cagagcacaa
    49561 tggcgcttcg cgcgagcatt cgccaccacg cggcgaagca gtcagcagaa ttacagcagc
    49621 agcaacaaag cctgaatacc tggttacagg aacacgaccg cttccgtcag tggaacaacg
    49681 aactggcggg ttggcgtgcg cagttctccc aacaaaccag cgatcgcgag catctgcggc
    49741 aatggcagca acagttaacc catgctgagc aaaaacttaa tgcgcttgcg gcgatcacgt
    49801 tgacgttaac cgccgatgaa gttgctaccg ccctggcgca acatgctgag caacgcccac
    49861 tgcgtcagcg cctggtcgcg ctgcatgggc agattgttcc ccaacaaaaa cgtctggcgc
    49921 agttaatggt cactatccag aatggatctc tggaacagac gcaacgtaat gtcgcactta
    49981 acgaaatgcg ccagcgttat aaagaaaaga cgcagcaact tgccgatgtg aaaaccattt
    50041 gcgagcagga agcgcgcatc aaaacgctgg aagcccagcg tgcacagtta caggcgggtc
    50101 agccttgtcc actttgtggt tccaccagcc acccggcggt cgaggcgtat caggcgctgg
    50161 agcctggtgt taatcagtct cgattactgg cgctggaaaa cgaagttaaa aagctcggcg
    50221 aagaaggtgc ggcgctgcgc gggcaactgg atgcattaac gaagcagctt cagcgcgatg
    50281 aaaacgaagc gcaaagcctc cgacaagatg agcaagcact tactcaacaa tggcaagccg
    50341 tcacggccag cctcaatatc accttgcagc cacaggacga tattcaaccg tggctggatg
    50401 cacaagatga gcacgaacgc cagctgcggt tactcagcca acgacatgaa ttacaagggc
    50461 agattgccgc gcataatcag caaattatcc agtatcaaca gcaaattgaa caacgccagc
    50521 agcagctctt aacggcattg gcgggttatg cactgacatt gccacaggaa gatgaagaag
    50581 agagctggtt ggcgacacgt cagcaagaag cgcagagctg gcagcaacgc cagaacgaat
    50641 taaccgcgct gcaaaaccgt attcagcagc tgacgccgat tctggaaacg ttgccgcaaa
    50701 gtgatgatct cccgcacagc gaagaaactg tggcgctgga taactggcgg caggttcatg
    50761 aacaatgtct cgcattacac agccagcagc agacgttaca gcaacaggat gttctggcgg
    50821 cgcaaagtct gcaaaaagcc caggcgcagt ttgacaccgc gctacaggcc agcgtctttg
    50881 acgatcagca ggcgttcctt gcggcgctaa tggatgaaca aacactaacg cagctggaac
    50941 agctcaagca gaatctggaa aaccagcgcc gtcaggcgca aacgctggtc actcagacag
    51001 cagaaacgct ggcacagcat caacaacacc gacctgacgg gttggctctc actgtgacgg
    51061 tggagcagat tcagcaagag ttagcgcaaa ctcaccaaaa gttgcgtgaa aacaccacga
    51121 gtcaaggcga gattcgccag cagctgaagc aggatgcaga taaccgtcag caacaacaaa
    51181 ccttaatgca gcaaattgct caaatgacgc agcaggttga ggactgggga tatctgaatt
    51241 cgctaatagg ttccaaagag ggcgataaat tccgcaagtt tgcccagggg ctgacgctgg
    51301 ataatttagt ccatctcgct aatcagcaac ttacccggct gcacgggcgc tatctgttac
    51361 agcgcaaagc cagcgaggcg ctggaagtcg aggttgttga tacctggcag gcagatgcgg
    51421 tacgcgatac ccgtaccctt tccggcggcg aaagtttcct cgttagtctg gcgctggcgc
    51481 tggcgctttc ggatctggtc agccataaaa cacgtattga ctcgctgttc cttgatgaag
    51541 gttttggcac gctggatagc gaaacgctgg ataccgccct tgatgcgctg gatgccctga
    51601 acgccagtgg caaaaccatc ggtgtgatta gccacgtaga agcgatgaaa gagcgtattc
    51661 cggtgcagat caaagtgaaa aagatcaacg gcctgggcta cagcaaactg gaaagtacgt
    51721 ttgcagtgaa ataactattc agcaggataa tgaatacaga ggggcgaatt atctcttggc
    51781 cttgctggtc gttatcctgc aagctatcac tttattggct acggtgattg gtagccgttc
    51841 tggtggttgt gatggtggta tgaaaaaagt cattttatct ttggctctgg gcacgtttgg
    51901 tttggggatg gccgaatttg gcattatggg cgtgctcacg gagctggcgc ataacgtagg
    51961 aatttcgatt cctgctgccg ggcatatgat ctcgtattat gcactggggg tggtggtcgg
    52021 tgcgccaatc atcgcactct tttccagccg ctactcactc aaacatatct tgttgtttct
    52081 ggtggcgttg tgcgtcattg gcaacgccat gttcacgctc tcttcgtctt acctcatgct
    52141 cgccattggt cggctggtat ccggctttcc gcatggcgca ttttttggcg tcggcgcgat
    52201 cgtgttatca aaaattatca aacccggaaa agtcaccgcc gccgtggcgg ggatggtttc
    52261 cgggatgaca gtcgccaatt tgctgggcat tccgctggga acgtatttaa gtcaggaatt
    52321 tagctggcgt tacacctttt tattgatcgc tgtttttaat attgcggtga tggcatcggt
    52381 ctatttttgg gtgccggata ttcgcgacga ggcgaaagga aagctgcgcg aacaatttca
    52441 ctttttgcgc agcccggccc cgtggttaat tttcgccgcc accatgtttg gcaacgcagg
    52501 tgtgtttgcc tggttcagct acgtaaagcc atacatgatg tttatttccg gtttttcgga
    52561 aacggcgatg acctttatta tgatgttagt tgggctaggg atggtgctgg gaaatatgct
    52621 aagtggcagg atttcaggac gttattcacc actgcgtatt gcagcagtga ctgactttat
    52681 aattgtactg gcactgctga tgctcttttt ctgcggcggc atgaaaacaa cgtcgcttat
    52741 ttttgctttt atttgttgcg cgggattatt tgccctttca gcaccgctac aaatattgtt
    52801 actacaaaac gccaaaggcg gagagttatt aggtgccgca ggtgggcaaa tagcgtttaa
    52861 cctcggtagc gccgtcggcg catattgcgg tggtatgatg ctgacgctgg ggctggcata
    52921 taattacgtg gcgctgcctg ccgccctgct ttcgtttgct gcgatgtcgt cgttgctact
    52981 gtatggtcgc tataagcgcc agcaagcggc ggatactccg gtgctggcga aaccactggg
    53041 gtaggttata gtctcggtgt tcattactta ttgccggatg cggcgtgaac gccttatccg
    53101 ccctacgcgg ttctggcaca ttttgcaggc ctgataagac gcggcaagcg tcgcatcagg
    53161 catgatgcgc caattgccta cgttttttac tcttgtggcc ataaccacgc agcgccgcgt
    53221 acgccgctgg aatcaccgtg cttcgcctta cgcaccggcg tttcacattc gccgccgaag
    53281 acaaattgtt taatcaactg cggaaccgtt tgatataaac ggtctacatt gctcat
//
LOCUS       DAJTNA010000006        50352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTNA010000006 DAJTNA010000000
VERSION     DAJTNA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50352
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.006"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(208..414)
                     /locus_tag="OO161_000277"
     CDS             complement(208..414)
                     /locus_tag="OO161_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5461479.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            727..816
                     /gene="kdpF"
                     /locus_tag="OO161_000278"
     CDS             727..816
                     /gene="kdpF"
                     /locus_tag="OO161_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5461480.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            816..2479
                     /gene="kdpA"
                     /locus_tag="OO161_000279"
                     /pseudo
     CDS             816..2479
                     /gene="kdpA"
                     /locus_tag="OO161_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2502..4550
                     /gene="kdpB"
                     /locus_tag="OO161_000280"
     CDS             2502..4550
                     /gene="kdpB"
                     /locus_tag="OO161_000280"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR5461481.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFPRKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGEIFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVGDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4559..5102
                     /gene="kdpC"
                     /locus_tag="OO161_000281"
                     /pseudo
     CDS             4559..5102
                     /gene="kdpC"
                     /locus_tag="OO161_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            5095..7779
                     /gene="kdpD"
                     /locus_tag="OO161_000282"
     CDS             5095..7779
                     /gene="kdpD"
                     /locus_tag="OO161_000282"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR5461482.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7776..8453
                     /gene="kdpE"
                     /locus_tag="OO161_000283"
     CDS             7776..8453
                     /gene="kdpE"
                     /locus_tag="OO161_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR5461483.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMRHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8669..8926)
                     /locus_tag="OO161_000284"
     CDS             complement(8669..8926)
                     /locus_tag="OO161_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461484.1"
                     /translation="MQKPLPVRWLIIQELTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            9049..11247
                     /gene="speF"
                     /locus_tag="OO161_000285"
     CDS             9049..11247
                     /gene="speF"
                     /locus_tag="OO161_000285"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR5461485.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCNLNSILFLLTPAEYMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11244..12563
                     /gene="potE"
                     /locus_tag="OO161_000286"
     CDS             11244..12563
                     /gene="potE"
                     /locus_tag="OO161_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5461486.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPCFELKNKHG"
     gene            12639..12803
                     /locus_tag="OO161_000287"
                     /pseudo
     CDS             12639..12803
                     /locus_tag="OO161_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="response regulator in two-component regulatory
                     system with KdpD; regulates the kdp operon involved in
                     potassium transport; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            12927..13577
                     /locus_tag="OO161_000288"
     CDS             12927..13577
                     /locus_tag="OO161_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HCR5461487.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(13618..14112)
                     /locus_tag="OO161_000289"
     CDS             complement(13618..14112)
                     /locus_tag="OO161_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461488.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     GLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(14327..15967)
                     /gene="pgm"
                     /locus_tag="OO161_000290"
     CDS             complement(14327..15967)
                     /gene="pgm"
                     /locus_tag="OO161_000290"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5461489.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(15993..16538)
                     /gene="seqA"
                     /locus_tag="OO161_000291"
     CDS             complement(15993..16538)
                     /gene="seqA"
                     /locus_tag="OO161_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5461490.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            16723..17487
                     /gene="ybfF"
                     /locus_tag="OO161_000292"
     CDS             16723..17487
                     /gene="ybfF"
                     /locus_tag="OO161_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5461491.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            17627..17920
                     /gene="ybfE"
                     /locus_tag="OO161_000293"
     CDS             17627..17920
                     /gene="ybfE"
                     /locus_tag="OO161_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5461492.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            18060..18590
                     /gene="fldA"
                     /locus_tag="OO161_000294"
     CDS             18060..18590
                     /gene="fldA"
                     /locus_tag="OO161_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5461493.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            18800..18886
                     /gene="uof"
                     /locus_tag="OO161_000295"
     CDS             18800..18886
                     /gene="uof"
                     /locus_tag="OO161_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5461494.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            18879..19325
                     /gene="fur"
                     /locus_tag="OO161_000296"
     CDS             18879..19325
                     /gene="fur"
                     /locus_tag="OO161_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5461495.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(19410..19736)
                     /locus_tag="OO161_000297"
     CDS             complement(19410..19736)
                     /locus_tag="OO161_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461496.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKAYCDKVWQE
                     IRSNNK"
     gene            complement(19786..21176)
                     /gene="chiP"
                     /locus_tag="OO161_000298"
                     /pseudo
     CDS             complement(19786..21176)
                     /gene="chiP"
                     /locus_tag="OO161_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(21623..23287)
                     /gene="glnS"
                     /locus_tag="OO161_000299"
     CDS             complement(21623..23287)
                     /gene="glnS"
                     /locus_tag="OO161_000299"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5461497.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(23490..25436)
                     /gene="nagE"
                     /locus_tag="OO161_000300"
     CDS             complement(23490..25436)
                     /gene="nagE"
                     /locus_tag="OO161_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5461498.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            25769..26569
                     /gene="nagB"
                     /locus_tag="OO161_000301"
     CDS             25769..26569
                     /gene="nagB"
                     /locus_tag="OO161_000301"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5461499.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            26629..27777
                     /gene="nagA"
                     /locus_tag="OO161_000302"
     CDS             26629..27777
                     /gene="nagA"
                     /locus_tag="OO161_000302"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5461500.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            27786..29006
                     /gene="nagC"
                     /locus_tag="OO161_000303"
                     /gene_synonym="nagR"
     CDS             27786..29006
                     /gene="nagC"
                     /locus_tag="OO161_000303"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5461501.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            29054..29806
                     /gene="nagD"
                     /locus_tag="OO161_000304"
     CDS             29054..29806
                     /gene="nagD"
                     /locus_tag="OO161_000304"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR5461502.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            30203..31867
                     /gene="asnB"
                     /locus_tag="OO161_000305"
     CDS             30203..31867
                     /gene="asnB"
                     /locus_tag="OO161_000305"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5461503.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKNILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            32248..32324
                     /locus_tag="OO161_000306"
     tRNA            32248..32324
                     /locus_tag="OO161_000306"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32282..32284,aa:Met,seq:cat)
     gene            32334..32418
                     /locus_tag="OO161_000307"
     tRNA            32334..32418
                     /locus_tag="OO161_000307"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32368..32370,aa:Leu,seq:tag)
     gene            32442..32516
                     /locus_tag="OO161_000308"
     tRNA            32442..32516
                     /locus_tag="OO161_000308"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32474..32476,aa:Gln,seq:ttg)
     gene            32551..32625
                     /locus_tag="OO161_000309"
     tRNA            32551..32625
                     /locus_tag="OO161_000309"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32583..32585,aa:Gln,seq:ttg)
     gene            32641..32717
                     /locus_tag="OO161_000310"
     tRNA            32641..32717
                     /locus_tag="OO161_000310"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32675..32677,aa:Met,seq:cat)
     gene            32766..32840
                     /locus_tag="OO161_000311"
     tRNA            32766..32840
                     /locus_tag="OO161_000311"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32798..32800,aa:Gln,seq:ctg)
     gene            32878..32952
                     /locus_tag="OO161_000312"
     tRNA            32878..32952
                     /locus_tag="OO161_000312"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32910..32912,aa:Gln,seq:ctg)
     gene            complement(33106..34281)
                     /gene="ubiF"
                     /locus_tag="OO161_000313"
     CDS             complement(33106..34281)
                     /gene="ubiF"
                     /locus_tag="OO161_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5461504.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGAKSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFSSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            34427..35851
                     /gene="miaB"
                     /locus_tag="OO161_000314"
     CDS             34427..35851
                     /gene="miaB"
                     /locus_tag="OO161_000314"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5461505.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRVARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            36004..37044
                     /locus_tag="OO161_000315"
     CDS             36004..37044
                     /locus_tag="OO161_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5461506.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            37041..37508
                     /gene="ybeY"
                     /locus_tag="OO161_000316"
     CDS             37041..37508
                     /gene="ybeY"
                     /locus_tag="OO161_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5461507.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            37598..38476
                     /gene="corC"
                     /locus_tag="OO161_000317"
     CDS             37598..38476
                     /gene="corC"
                     /locus_tag="OO161_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5461508.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            38501..40039
                     /gene="lnt"
                     /locus_tag="OO161_000318"
     CDS             38501..40039
                     /gene="lnt"
                     /locus_tag="OO161_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5461509.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(40117..41994)
                     /locus_tag="OO161_000319"
     CDS             complement(40117..41994)
                     /locus_tag="OO161_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR5461510.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLTLSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            42449..43357
                     /gene="gltI"
                     /locus_tag="OO161_000320"
     CDS             42449..43357
                     /gene="gltI"
                     /locus_tag="OO161_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5461511.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            43527..44267
                     /gene="gltJ"
                     /locus_tag="OO161_000321"
     CDS             43527..44267
                     /gene="gltJ"
                     /locus_tag="OO161_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5461512.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSVIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            44267..44941
                     /gene="gltK"
                     /locus_tag="OO161_000322"
                     /pseudo
     CDS             44267..44941
                     /gene="gltK"
                     /locus_tag="OO161_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
     gene            44941..45666
                     /gene="gltL"
                     /locus_tag="OO161_000323"
     CDS             44941..45666
                     /gene="gltL"
                     /locus_tag="OO161_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5461513.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            45784..46719
                     /gene="rihA"
                     /locus_tag="OO161_000324"
     CDS             45784..46719
                     /gene="rihA"
                     /locus_tag="OO161_000324"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5461514.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            46803..48497
                     /gene="hscC"
                     /locus_tag="OO161_000325"
     CDS             46803..48497
                     /gene="hscC"
                     /locus_tag="OO161_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR5461515.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLKDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIPLTRLPDAA"
     gene            complement(48533..49984)
                     /gene="djlC"
                     /locus_tag="OO161_000326"
                     /pseudo
     CDS             complement(48533..49984)
                     /gene="djlC"
                     /locus_tag="OO161_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(49981..>50352)
                     /locus_tag="OO161_000327"
     CDS             complement(49981..>50352)
                     /locus_tag="OO161_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5461516.1"
                     /translation="GIKAWDYVRMGVLSRVGVLNKWLTEEESLWLQSRVYVRAHHYYH
                     GWMHYFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATL
                     PWQPLTVQPECPVTLKDVSDL"
BASE COUNT        12150 a        12867 c        12903 g        12432 t
ORIGIN      
        1 gctgacaggc aaaaccctac agatttataa gaaagcctta agtgagatat ccatcagata
       61 gaaacaatta ggaaaatatt tatatatttt tcctgaatat atttcagctg ggtaatacca
      121 tatctaaata tttacctgaa catcttccta ataaaaaaac cgccagcatc tccactagcg
      181 gttttcaatg tattcaaaac tcggcaatca gtaaaaatca ccggttgcct tttcggcctg
      241 ttccatccaa accggtttat cgctggtttt cgaccagacg cggtgcaggt agctgtaaaa
      301 gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc
      361 atatgtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt ccatactttt
      421 ctcccccttt tgtatctacc cggtgaatag caccggaaaa atgaatttgt ttatctgatg
      481 aaaatagtac cgccttttgt gtaattttac tactcatccg accacttatt tttgcttatt
      541 gatggtttat ttacattcac tctgcaatta agtcacacaa aagttaaatt aatgctaaac
      601 attagttaaa tcatggcttt tgccattttt atactttttt tacaccccgc ccgcagattt
      661 ttgcgaaatc tttgcagcca gaattctact cttccggtat cacttttagg ccactggagg
      721 tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg tgtttttatt actgggttat
      781 ttggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga
      841 ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc
      901 ggctgattaa tgacattcct cttccaggta cagcgggcgt tgaacgtata ctctttcgtt
      961 tacctggcgt ctctgaccat gagatgaact ggaagcaata tctttgtgcc attctcggcc
     1021 tgaacatgct ggggctggcg gtgctgtttt ttatgttgct cggtcagcac tatctgccgc
     1081 ttaatccaca gcagttgccg gggctgtcgt gggatctggc gctgaatacc gccgtcagct
     1141 ttgtcacgaa taccaactgg caatcttata gcggtgaaac cacgttgagc tatttcagcc
     1201 agatggcagg cttaacggta caaaactttc tttctgccgc cagcgggatt gcggtgattt
     1261 ttgccttcat ccgtgcgttt actcgccaga gcatgagcac actcgggaat gcctgggtcg
     1321 atctgctacg catcacgtta tgggtgctag tccctgtggc gttgttgatc gcactgtttt
     1381 ttattcaaca aggtgcgctg caaaactttc tgccctatca ggcagtgaat accgttgaag
     1441 gagcgcaaca gctgttaccc atgggaccag tagcttctca ggaagcgatc aagatgctcg
     1501 gtactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa aacccaaccg
     1561 cactgaccaa cttcgtgcag atgctggcat cccaacagct ctgtgctttg cctttggtga
     1621 agtgacgggc gatcgccgcc aggggcgcat gttgctgtgg gcgatgtcag tgatttttgt
     1681 catctgcgta ggcgtggtta tgtgggcaga agttcagggt aatcctcatc tgctggcgct
     1741 cggcgcagac agcagcatca atatggaagg taaagagagc cgtttcggcg tgctggtcag
     1801 tagcctgttt gcggtcgtga ctacggcggc gtcctgtggc gcggtgattg cgatgcatga
     1861 ttcgtttacc gctctcggcg gcatggtgcc gatgtggcta atgcaaattg gtgaagtggt
     1921 gttcggcggt gtcggttctg gtctttacgg catgatgctg tttgtcctgc tggcggtgtt
     1981 tattgccggg ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa tcgacgtacg
     2041 cgagatgaaa ctgactgcac tggcgattct ggttactcca acactggtac tgatgggcgc
     2101 ggcgttggcg atgatgaccg acgccggacg tagcgccatg cttaaccctg gcccgcatgg
     2161 ttttagcgaa gtgctgtacg ccgtgtcgtc cgccgctaac aacaacggca gcgcctttgc
     2221 cggattaagc gccaactctc cgttctggaa ctgtttactg gcgttctgca tgtttgtcgg
     2281 tcgcttcggg gtgattatcc cggtgatggc aattgccggt tcgctggtga gtaaaaagag
     2341 ccaacccgcc agctccggca cgctgccaac gcacggcccg ctgtttgttg gcccgttaat
     2401 cggcaccgtg ttgctggttg gcgcactgac ctttatccct gccctggcgc ttggtccggt
     2461 ggcggaatat ctctcctgat gatattgagt gagcactgaa tatgagtcgt aaacaactgg
     2521 cgctattcga accaacactt gtcgttcagg cgctgaaaga agcggtgaaa aaattaaacc
     2581 cgcaggcgca atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt ctgctgacca
     2641 cctgtattag catcgcgatg gcaagcggtg cgatgcctgg caatgcgctg tttagcgcgg
     2701 caattagcgg ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag gcgctggcag
     2761 aaggccgcag taaagcgcag gccaacagtc ttaaaggggt gaaaaaaact gcctttcccc
     2821 gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa agttcctgcc gatcaactgc
     2881 gtaaaggtga catcgtgctg gtagaagctg gcgatattat cccctgcgat ggtgaagtca
     2941 ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca ccggtgatcc
     3001 gtgaatccgg cggcgatttt gcctccgtca ccggcggcac gcgtattctt tctgactggc
     3061 tggtgattga gtgtagcgtt aaccccggtg agatatttct ggatcggatg atcgcgatgg
     3121 tggaaggcgc acagcgacgc aaaacaccga acgagattgc cctgactatc ctgctgattg
     3181 ctctgaccat cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc gcgtggggcg
     3241 gtaatgccgt gagtgtaacg gtactggtgg cgctgctggt ctgtctgatc ccaaccacta
     3301 ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag tcggatgctg ggcgcgaatg
     3361 tgattgccac cagcggacgt gcagttgaag cggcaggtga cgttgacgtt ctgctactgg
     3421 ataaaaccgg caccatcaca ctcggtaacc gtcaggcgtc ggagtttatc cctgcgcagg
     3481 gcgtggatga aaaaacgctg gctgacgccg cacaactggc ttcgctggct gatgaaacgc
     3541 cggaaggccg cagtattgtg atcctcgcca agcaacgttt taacctgcgc gagcgcgatg
     3601 tgcagtcgct ccatgccacc tttgtaccgt ttactgcgca aagccggatg agcgggatca
     3661 acatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc catgttgagg
     3721 ctaacggcgg tcacttccct gccgatgttg atcaaaaagt cgatcaggtt gcgcgtcagg
     3781 gggccacgcc gctggtggtg gtggaaggtt cccgtgtgct gggcgttatt gcgctgaaag
     3841 atatcgtcaa aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg ggcattaaaa
     3901 cggtgatgat taccggcgat aaccgtctga ctgccgccgc cattgctgcg gaagcgggcg
     3961 tcggtgattt tctcgccgaa gcgacaccgg aggccaagct ggcattgatt cgtcagtatc
     4021 aggcggaagg ccgtttggtg gcgatgaccg gcgacggcac caacgatgct ccggcgctgg
     4081 cgcaagcaga tgtcgcggtg gcgatgaact ccggcaccca ggcggcgaaa gaggcgggca
     4141 atatggtcga tctcgactct aacccgacca agttgatcga ggtggtgcat attggcaaac
     4201 agatgctgat gacccgtggc tcgctgacca ccttcagcat tgccaacgat gtggcgaaat
     4261 acttcgccat tattccggcg gcgttcgcgg caacgtatcc gcagttaaat gcgctgaaca
     4321 tcatgcgcct gcattcgccc gactccgcca ttctcagtgc agtcattttc aacgccttga
     4381 ttatcgtctt tttgattcct ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt
     4441 ctgccatgtt gcgccgtaac ttatggattt acggtctggg tgggctgctg gtgccgttta
     4501 ttggtatcaa agtcattgat ttactgctga ccgtttgcgg tctggtgtga ggtttaccat
     4561 gcgtggctta cgtccggcat tatcgacatt tatctttctg ttattgatta ctggcggcgt
     4621 ttacccgctg ctgaccaccg tgctggggca atggtggttt ccctggcagg ccaacggttc
     4681 gttgattcgt gaaggtgata cggtgcgggg ttcggcatta atcgggcaga attttaccgg
     4741 caacggcaga aatgccctat aatccacagg cttctggcgg gagcaatctg gcggtcagta
     4801 accctgagct ggataaacaa atagccgcac gcgttgctgc attacgggcc gctaacccgg
     4861 atgccagtac gaacgttccg gttgaactgg tgacggcatc ggcaagcggg ctggacaata
     4921 atatcacccc gcaagcggcg gcctggcaga tcccacgcat cgcgaaagcg cgtaatctca
     4981 gcgttgaaca gctcacgcag ctgatcgcaa aatacagcca acaaccgctg gtgaaatata
     5041 tcggccagcc ggtcgtcaac attgtcgaac tcaatctggc gctggataag cttgatgaat
     5101 aacgaaccct tacgtcccga ccccgatcgt ctgctggaac aaactgccgc gccgcatcgg
     5161 gggaagctga aagttttctt cggtgcctgt gcaggcgtcg ggaagacctg ggcgatgctg
     5221 gcagaagccc agcgactgcg ggcgcaaggg ctggatattg tggttggcgt ggtagaaacc
     5281 cacgggcgaa aagataccgc cgcaatgctg gaagggctgg ctgttctgcc gccaaaacgc
     5341 ctggcgtacc gtgggcggca catctgcgag tttgacctcg atgccgccct cgcccgccgt
     5401 ccggcgctga tcttaatgga cgaactggcg cacagtaatg cgccaggttc tcgtcaccct
     5461 aaacgctggc aggacatcga agagctgctg gaagcaggca ttgatgtttt cactaccgtc
     5521 aacgttcagc atctggaaag tctgaatgat gtggtcagcg gcgtcaccgg aattcaggta
     5581 cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg acgtggttct ggtggacttg
     5641 cccccggacg atctgcgcca gcggctgaaa gaaggcaaag tctatattgc cgggcaggcg
     5701 gagcgcgcca ttgaacattt tttccgcaaa ggcaatctga tcgccctgcg cgaactggca
     5761 ctgcgccgta ctgccgatcg cgttgatgag caaatgcgcg cctggcgggg gcatcctggc
     5821 gaagagaaag tctggcacac gcgcgacgcg atccttttat gcatcggcca taacaccggt
     5881 agtgagaagc tggtccgcgc ggcggcgcgg ctggcgtcac ggctgggtag cgtctggcac
     5941 gcggtgtatg ttgaaacccc tgccctgcac cgcttaccgg aaaaaaaacg tcgggcaatt
     6001 ctcagcgcct tacgtctggc gcaggagctg ggcgcggaga cggcaacact ttctgatcca
     6061 gcggaagaaa aagcagtagt gcgttatgcc cgtgaacata atctcggcaa gattattctc
     6121 ggtcgcccgg cctcgcgccg ctggtggcgt cgggaaacgt ttgctgaccg actggcgcgc
     6181 atcgcccccg atctcgatca ggtgctggtc gcgcttgatg aaccacccgc acgcacgatt
     6241 aacaacgcgc cggatagccg ctcttttaaa gacaagtggc gggtacagat tcagggatgc
     6301 gtggttgccg ccgcgttatg cgccgttatc actttaattg ccatgcagtg gctgatggcg
     6361 tttgatgccg ccaacctggt gatgctgtat ctgcttggcg tggtggtggt ggcgctattt
     6421 tatggacgct ggccttcggt ggttgctacc gtcattaatg tggtgagttt cgatctcttt
     6481 tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc aatatctgct gaccttcgca
     6541 gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg ctggcgtgcg ttatcaggcg
     6601 cgggtagccc gttaccgcga gcaacgcaca cggcacttat atgaaatgtc gaaagctctg
     6661 gcggtgggcc gcagtccgca ggacatcgcc gccaccatcg aacaatttat tgcctccacg
     6721 tttcatgccc gcagtcaggt gttgttgccc gatgacaacg gtaaattgca gccgttaaca
     6781 catccgcaag gaatgacgcc atgggacgat accatcgcgc agtggagttt tgataaaggc
     6841 ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac cgtaccagat tttgccgcta
     6901 aaaagcggcg agaaaaccta cgggctggtg gtggtggagc cggggaatct gcgccagttg
     6961 atgatcccgg aacagcagcg cctgctggag acgtttacgc tgttagtcgc caatgccctt
     7021 gagcggctga cgctaaccgc cagcgaagaa caggcgcgga tggcaagcga acgtgaacag
     7081 atccgcaacg ccctgctggc agcgctttcg catgatttac gcacgccgct cacggtgctg
     7141 ttcggtcagg cagaaatctt aacgctcgat ctggcaagcg aaggatcgcc ccacgcccgc
     7201 caggccagcg agattcgtca gcatgtgctg aacactaccc gactggtgaa taatctactg
     7261 gatatggcgc gaattcagtc cggcggcttt aatttgaaga aagagtggtt aacgctggaa
     7321 gaagtggtcg gtagcgcgct gcaaatgctg gaaccgggtt tatcgtcgcc catcaatctt
     7381 tctctgccag aaccgctgac cttaatccac gttgacgggc cactctttga acgggtgctg
     7441 attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc aggccgaaat tggtatcgat
     7501 gcccacgttg agggcgaaaa tctacaactg gatgtctggg ataacggccc cggtcttccg
     7561 ccaggccagg agcagacgat atttgataag tttgctcgcg ggaataaaga gtcggcagta
     7621 ccgggggtag ggcttggact ggcaatctgt cgggcgatag tggatgtaca cgggggcact
     7681 attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc gtgttacact tccccagcaa
     7741 actgcccctg aacttgaaga atttcatgag gatatgtgac aaacgttctg attgttgaag
     7801 atgaacaggc tattcgtcgc tttctgcgca cggcgctgga gggcgacggg atgcgcgtct
     7861 atgaggccga aacgctgcaa cgcggcttgc tggaagcggc tacccgtaag ccagatttga
     7921 ttattctcga tctcggcctg cccgatggtg atgggattga gtttatccgc gacctgcgcc
     7981 agtggagcgc ggtgccggtg attgtgcttt ccgcacgcag cgaagagagc gacaaaatcg
     8041 ccgcgctgga tgccggagcg gatgattatc tgagtaagcc gtttggcatt ggcgaattgc
     8101 aggcccgtct gcgcgtcgct ttacgccgcc actccgccac cgccgcgccc gatccgctgg
     8161 tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt gattcaccgg ggtgaggaag
     8221 aggtgcatct cacaccaatt gagttccacc tgctggcggt gctgctcaac aatgccggaa
     8281 aagtactcac ccagcgccag ctccttaacc aggtgtgggg gccaaacgcg gtcgaacaca
     8341 gtcactattt gcgtatttat atgagacatc tgcgacaaaa actggaacag gatcccgccc
     8401 gcccacgcca tttcattact gaaaccggta ttggctatcg gtttatgctt tgaatattaa
     8461 ttttaataca gcctgccttt tattaattaa agccgtaata ataaatacgg ctttttatct
     8521 taaacaacac acaaaaataa caattcaata ttttatatta ctgagtaaaa agctcatcat
     8581 taaataaatt aagattgatc attttttatt gatcaccttc acagttcaac cgtatttcct
     8641 ggatagaata tcttcacctt cattcacatc aggaaaggta aattaaatgg aaaataacag
     8701 ccgcactatg ccccatataa ggcggacaac tcatattatg aagtttgctc atcgcaatag
     8761 cttcgacttt cacttcttca atgcccgtta gtctaccgac taagggcact tcagcgtaca
     8821 ggtcttcctg actctctgta ttacaggcat atgcctgtat tccgcagtgc tcattctctg
     8881 ttaactcttg tattatcagc caccggactg gtaagggttt ctgcattctg aaaaaagcaa
     8941 tctacctgat ttatttatca gcggggtaac tttgcttttt tccggcacga tcgatttctc
     9001 attcgagaaa ttgaggacct gctattacct aaaataaaga gatgaaaaat gtcaaaatta
     9061 aaaattgcgg ttagtgattc ttgcccggac tgttttacca cgcagcgaga atgtatctac
     9121 attaatgaaa gtcgtaatat cgatgtggcg gcaatcgttt tatcgctcaa cgatgttaca
     9181 tgcggaaaac tcgatgaaat cgatgccacg ggttatggca tcccggtatt tattgctact
     9241 gaaaatcaag aacgtgtacc cgcagagtat ttgccccgta tttcgggtgt ctttgagaat
     9301 tgcgaatcgc gacgagaatt ttatggtcgt cagttagaaa ccgctgccag ccattatgaa
     9361 actcaactgc gcccaccttt cttccgcgca ctggtcgatt atgtcaatca aggtaacagc
     9421 gcgtttgatt gccctggtca tcagggcggc gaatttttcc gtcgccatcc ggcggggaat
     9481 cagtttgtgg aatactttgg tgaggcgctg ttccgttccg acttgtgcaa cgcagacgta
     9541 gcaatgggtg atctgctgat tcacgaaggc gcgccatgca ttgcacagca acatgcggca
     9601 aaagtgttca atgccgataa aacatacttc gttctaaatg gcacttcatc ttctaacaaa
     9661 gtggttttaa acgccctgct gacaccgggt gatctggtgc tgtttgaccg caataaccac
     9721 aaatctaacc accacggagc gttgctacag gctggtgcaa caccggttta tctggaaacc
     9781 gcacgtaacc cgtatggctt tatcggtggc attgatgcgc actgttttga agaaagttac
     9841 ctgcgtgagc tgatcgcgga agtcgcaccg cagcggacaa aagaggctcg tcctttccgc
     9901 ctcgccgtta ttcagctagg cacctacgac ggtacgattt ataacgcccg ccaggtggtg
     9961 gataaaattg gtcatctgtg tgactacatt ctgtttgact ccgcatgggt cggctatgag
    10021 caatttatcc cgatgatggc tgactgttca ccgctgttgc tggatcttaa tgagaacgat
    10081 ccgggcattc tggttacgca atctgtgcat aaacagcagg ctggtttttc tcaaacttca
    10141 caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc
    10201 atgaacaacg cctttatgat gcatgcctcc accagcccgt tctatccgct gtttgccgca
    10261 ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt
    10321 gtggtaaatg gcattaatgc ccgcaagctg atcctcgata actgtcagca tattcgtccg
    10381 ttcgtacctg aactagtgga tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg
    10441 gttgatctgc gcttcttcca gtttgtacca ggggaacact ggcactcttt tgaaggctat
    10501 gcagagaatc aatactttgt cgatccatgc aaactgttgc tgacaacccc aggtattgat
    10561 gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc taacttcctg
    10621 cgcgaaaatg gcgtagtgcc ggaaaaatgc aatcttaact ccatcctctt cctgctgact
    10681 ccggcagaat atatggccaa acttcagcaa cttgttgccc tgctggtacg cttcgaaaaa
    10741 ctgcttgagt ccgacgcgcc attagcagaa gtgctgcctt ccatctacaa acagcatgaa
    10801 gagcgctacg ccggttatac ccttcgtcag ttgtgccagg aaatgcatga tttgtatgcc
    10861 cgtcacaacg tgaaacaacc gcaaaaagag atgttccgta aggaacactt cccacgcgta
    10921 agcatgaatc cacaagaagc caactacgcc tatttacgcg gtgaagtgga gctggttcgt
    10981 ctgccggatg ccgaaggccg tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg
    11041 ctgtgtgttg ttccgggtga aatctggggt ggtgctgttt tgcgttactt cagcgctctg
    11101 gaagaaggga tcaacctgct gccaggtttt gcaccggagc tgcagggtgt ctatatcgaa
    11161 gaacatgatg gtcgtaagca agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc
    11221 accctgttga aaggggaaaa attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc
    11281 agttaaccat actgacgatg gtcaacatga tgggctccgg tatcatcatg ctgccgacaa
    11341 agcttgccga agttgggaca atctcaatta tctcctggct ggtgacagcc gtaggctcaa
    11401 tggcactggc atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca ggcggtatgg
    11461 gcggttatgc ggagtatgct ttcggtaagt ccggcaattt tatggcgaac tacacctacg
    11521 gtgtctcgct gctgattgct aacgtcgcga ttgctatttc ggcggttggt tacggcaccg
    11581 aactgctcgg cgcgagtttg tcgccagtgc agattggtct tgcgaccatc ggagtgctgt
    11641 ggatttgtac cgtggctaac tttggtggtg cgcgcattac cgggcaaatc agtagcatta
    11701 ctgtgtgggg agtcattatt ccggtcgtcg gtctgtgcat tattggctgg ttctggttta
    11761 gtccgacgct gtacgctaac tcatggaacc cgcaccatgt gccgttcttt agcgctgtag
    11821 gttcttccat cgccatgacg ttgtgggctt tcctcggtct ggagtcagcc tgtgccaacg
    11881 ccgaagtagt ggaaaacccg gaacgtaacg tgccgattgc agttcttggc ggtactttag
    11941 gtgcggcggt gatttatatc gtctccacca acgtgattgc cgggattgtg cctaatatgg
    12001 agctggcaaa ttcaacggca ccatttggcc tggctttcgc acagatgttc acaccagaag
    12061 tgggtaaagt cattatggcg ctgatggtga tgtcctgctg cggttcactg cttggctggc
    12121 agttcaccat tgcccaggtg tttaaatctt catctgatga aggctacttc cctaaaattt
    12181 tctcccgtgt aaccaaagtg gatgcaccgg tgcagggaat gttgaccatt gtgattattc
    12241 agagtggatt ggcactgatg accattagcc cgtcactgaa cagccagttc aacgtgctgg
    12301 ttaacctggc cgtggtgacc aatatcattc cgtatattct gtcaatggcg gcattagtca
    12361 ttattcagaa ggtcgctaat gtgcccccct caaaagcgaa agtagcaaac tttgttgcct
    12421 ttgttggtgc gatgtacagt ttctatgcgc tgtactcatc cggggaagaa gccatgctgt
    12481 acggttctat cgtgaccttc cttggttgga cactgtatgg tctggtatca ccatgctttg
    12541 aactgaaaaa taaacacggt taataaaaca aagtaaaaaa gggcgattga atgatcgccc
    12601 tttctgtttg ttggcaaata gttcgttatt tatgccgcat ccggcagatg tagggtctaa
    12661 acgcgatcga acacagcaac tatttgcgta attatatggg gcatctgcga caaaaactgg
    12721 aacaggatcc ggcccggccc ggcccggccc ggcctcgcca ttttattact gaaaccggta
    12781 ttgggtatcg gtttatgctt tgaaaaaagt ggcaatgtat ggataaagtc attttttatt
    12841 caaatataag gaacttaata tttaaaaaat gttccataca attcctcatc ataaatgaca
    12901 ccgtagcaca caaggagcaa gtagcgatgc gtgaaatgaa tcagttagta aatactgaag
    12961 attcagcctg gccaataatc cagaactggt taaaagacgc cacaaaccat accgaactct
    13021 taccggttaa taaaaattta gccgaaaccg cactttatca attacaggtg acaacaaaat
    13081 caccgatggg tgcgcttgtc tatggctccg gaggtctgct gattgataat ggttggttac
    13141 gcatagcagg atctggtcac ccccggttgc cacgagatcc agtaagttgg acacaacgcc
    13201 ctgaattcgc aggcgtgcgg gctttaccca tcgctgacga tgtcgcaggg ggaatttttg
    13261 ctcttaatgg tggcgatctt ggggaagata ctggctgcgt ttattatttt gcgccagata
    13321 cgctgaattg ggaaagtctg gaagttggat attctgagtt tcttcagtgg gcattatccg
    13381 gtgatttaga taccttctat gaaaatgtac gatggcagca atggcgagaa gacgtaataa
    13441 aattatcggc aacggaagcc ttcacgttct accctttctt atgggtacaa agtgaagaag
    13501 caagaacaag aaaagttatt tcgcttactg aactctggga aatgcagtat caaatgaaag
    13561 aaacattcac gcaataagac atgttcaggt ccgggtttca ttcccggact cactatttta
    13621 atgtgtattt atcgctttca attcttgctg gcaattttct gactggcagc gtcgtaactc
    13681 aataatcccg acatgcatca tcgagccaaa aaatccgctt tcgctgacat aatcctcatt
    13741 gactatcggt cgagtgccgg gcttgccctg gcaaacccgc ataaaaaatg aggtgtagca
    13801 ttgccaaccg ggatttaaac cgaccttttc ttgtgctgac atattatgaa gataagtaag
    13861 ttctttttcg ttcccgcctg acaactgcca gcggcgggta ttatcaggta agttatcacc
    13921 acggcaattt gtcttacacc gggaaggtgc aatatttacc gcaatgctac ccgttaatcc
    13981 tctggtttta tctttagggg tcagtaacca gtcgccagct aatgatgatt gttcaggttg
    14041 ggcgcatgca gtcagcagta atgtggcggt aattagtgac actatgacga ccagactcct
    14101 gttggtcttc attgggattt ccttgagcta ctcagtttgt tgacaaagtg ttcgttgttt
    14161 ttgccggatg cggcgtgaac gccttatccg gcctacaaaa tctcgtaaat tcaatatatt
    14221 gcaccatcct cgtaggcctg ataagcgtag cgcatcaggc aattctgtgt ttgtcatacg
    14281 taaaaaaggg cgatcttccg accacccttt ttcttattta atgtgtttac gcgtttttca
    14341 gaacttcgct aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga
    14401 ggaagctttc gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc
    14461 agccgttgtc agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg
    14521 tcaggcgcgc ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca
    14581 gcttagacag cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg
    14641 gcgcaccaaa gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag
    14701 cggtgatttc cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg
    14761 tgccgtcgaa acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc
    14821 cgtcgaacag accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc
    14881 ccaagtcatt gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa
    14941 catctttgcc ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt
    15001 ggttcggatt catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt
    15061 tagcaaacgc cagatcgaac ttatcacgca gtgccagcag gcccgccatc gcacactcgg
    15121 aggagcagtc catacggatc gcgccgtctt tatcaaggtg cataaagcgg aaggtttgat
    15181 cgacctgatc gttaacgatg gtcaggttga ggttgtaata ctcgccaata cgcttccagt
    15241 attcgatacc ggaaccgccc agcggatcaa cgcccagcgt caggcccgct ttctgaatcg
    15301 ccgccatatc aacgatatcg gccagacctt ccacaaacgg ctgcaccaga tcctgctctt
    15361 tcacatgacc ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat
    15421 cggccagcag tgcgttggcc ctgtcttcca ccactttggt gacgttggta tcagccgggc
    15481 caccatttgg cggattgtac ttgataccac catcttccgg cgggttatgg gacggtgtaa
    15541 tcacgatacc gtctgccagc ggaccacctt ttttattgtg aaccaggatg gcattggaaa
    15601 cggcaggcgt tggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg
    15661 ccagcacttc cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca
    15721 catagcaagg gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg
    15781 ccagaatgtg cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac
    15841 cgaatttcac cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg
    15901 tcagttgggc gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga
    15961 ttgccattgc tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc
    16021 aattccgccg ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg
    16081 gtgttggtgt tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga
    16141 ttaccatttt tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc
    16201 aacgattccg ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc
    16261 agcataaagc gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca
    16321 cgcattgcgc gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt
    16381 gacgcaacgc gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc
    16441 aacatacgcc gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata
    16501 tagctgtaga gttcatcatc aacttcaatc gttttcatct taatccagtg cagtgtctta
    16561 gctgaataca agtcgccaag agtatatgaa taaatcccgg cagcggattg cgtcaagcct
    16621 ggcttgcaga aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa
    16681 cataataccc taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc
    16741 gcgcaaactg cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt
    16801 ggtagcctcg acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc
    16861 caggttgata tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg
    16921 atggcgcagg atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc
    16981 ggtcactcca tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc
    17041 gataaactgg tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag
    17101 attttcgcgg ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca
    17161 gcaataatgc gccagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt
    17221 gttgacgggg agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg
    17281 ggttgggaga aaatcccggc atgggatcac cctgccctgt ttatccctag cggcaattct
    17341 ccgtatgtta gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg
    17401 catgtgattg caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc
    17461 gctatccgtc gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc
    17521 tttccgcgcg tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat
    17581 ccgggcagag gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca
    17641 aacggaccgt acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac
    17701 taatccgctt tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga
    17761 caaagtacgt ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct
    17821 gaacgagctg gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat
    17881 gcagcaactg gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca
    17941 cagtgtgcag tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc
    18001 tttatccgtg ggcaatttcc cccccccatt tcaataagtt tcaagaggtt atttcactca
    18061 tggctatcac tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa
    18121 tgattcaaaa acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca
    18181 aagaagatct ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg
    18241 aagcgcagtg tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca
    18301 aactggttgc gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg
    18361 cattgggcac catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc
    18421 caactgcggg ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg
    18481 gtctggctat cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg
    18541 ttaaacagat ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg
    18601 cgtagactca tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat
    18661 ttgtaacttt tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt
    18721 aaatgtaagc tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt
    18781 taggcgtggc aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc
    18841 taatgaagtg aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc
    18901 taaagaaagc tggcctgaaa gtaacgcttc ctcgtttaaa aatcctggaa gttcttcagg
    18961 agccggacaa ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg
    19021 aagaaattgg tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg
    19081 tcacccgcca caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc
    19141 acgatcacct gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg
    19201 aagcgcgtca gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct
    19261 atctttacgg tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca
    19321 aataagccag cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa
    19381 ataagaaaag cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa
    19441 actttatcac aataggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac
    19501 tgctgataat ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct
    19561 ttcttcagca cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg
    19621 gtgttgatac aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat
    19681 tgcgcacaag ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca
    19741 aattcctcaa acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa
    19801 tggtgcgatt accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc
    19861 gccccagctt gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt
    19921 gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc
    19981 aatagtccgg tttttgtcgt agtacgcatc cggattgctc tgccaggtcg caggtttagc
    20041 atcccatgcg taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag
    20101 gtcatacatc gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt
    20161 atcccaccag atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag
    20221 gaagtatccc tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac
    20281 gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc
    20341 gttgacgctg cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa
    20401 cgggctaccg gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc
    20461 ttccgcctga ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc
    20521 aagggagtgc agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc
    20581 cagatgccac ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc
    20641 atcgccgtaa tcaaaattcg ccccggcttc cgccccctga taagtacctg cggcgctaac
    20701 agcgtttgac cagttggctg aatgtaacct gccctcgccc aaaccggacc gtatttaaat
    20761 ttggccgcag ctttatacag gcttataccg cttttgtcgc cggaccagtc ttcgtcatag
    20821 gctttattac tttttgaaaa cgcgatttcg ttcgggtggg agctgtcgcc gttttccgcc
    20881 atttcaatcg ccgtaaacac agcaatatca aggccgaaca tatcagcagc ataaccggac
    20941 tgaaaatcga ggttggcatt ccaggtggag tgagaaaggt tggttttgta tttgtcgccg
    21001 tcggtaacat ctttacggtc gcgttcacgc tgccagtaat atataccgcc ggttaaggtt
    21061 gaatcgtcga tgaatccttc ggcgcgagcc tccggcattg ccataaagcc cgacattgct
    21121 gtaacaccgg cgatagccag cgccagcgta ctacgtttgc cactaaacgt acgcatgggt
    21181 taatcctctt tgacgtataa attgctgcac caaaggtgaa cagcgaaaaa aataaaaatt
    21241 aaaaaagtag cattgagaaa agtctcgctg actacaggaa tagactattt ttcgcgacgc
    21301 gaattatcaa tctctttctg taaccagaat gtaagaacat gagcaaccgc acatattttg
    21361 atgatttttg ttacaaggct taagtttgcg gttgaaaggc ttgcagaaga ataaaagcgt
    21421 aaatttccag caagatgaaa gcggttacat tttatttaat tatggtttgt gattttaaat
    21481 ttattttatg gataaattgc gaagcggaat taattcgcga agaaaagcag aaaaaacgcc
    21541 gcagagtgcg gcgtttggga ttgtttgcta tttcttgccg gatgcgacgt aaatgcctca
    21601 cccggcctgg tagaacttaa aattactcgc ctactttcgc ccaggtatca cgcagcccaa
    21661 cggtgcggtt aaataccggt ttttccgctg tggaatggcg gctgtcgagg cagaagtaac
    21721 cttcacgctc aaactggaat gctttacccg caaccgcatc tttcagcgac ggttcagcaa
    21781 agccctgttt gatcaccagc gattccgggt taatcaccga caggaaatca tccgcagcac
    21841 ctgggttagg cacgctgaac agacggtcgt acagacggat ttcaaccggc agcgcatgtg
    21901 ccgcgctcac ccagtgaata acacctttga ctttacgacc atctgccgga tctttgctta
    21961 aggtgtcggc gtcataagta cagaagatgg tggtgatatt gccttcggca tctttctcca
    22021 cgcgttctgc cttaatgacg taagcattac gcagacgcac ttctttaccc agcaccagac
    22081 gtttgtactg cttgttagct tcttcgcgga aatcggcgcg atcgatccaa atctcaccgc
    22141 taaacggcac ctggcggcta cccatttccg gtttgttcgg atggttcggc atggtaacca
    22201 tttcgccttc gccctgatag ttttcgataa ccagtttcac cggatcgata accgccattg
    22261 cgcgcggggc gttttcgttg agatcttcac ggatgcagga ttccagcgac gccatctcaa
    22321 tggtgttgtc ctgcttggtc acgccgatgc gtttgcagaa ctcacgaata gaagcagcag
    22381 tgtaaccacg acgacgcaga ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa
    22441 cgtgcttgtc ggtcaccagc aggttcagct tacgcttgga catcacagtg tattccagat
    22501 tcaggcgcga gaattcgtac tggcgcgggt gaacaggaat ggtgatgttg tccagcaccc
    22561 agtcgtacag gcgacggttg tcctggaact caagcgtaca cagagagtgc gtaatacctt
    22621 ccagggcatc gctgatgcag tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc
    22681 cagtctggtg gtgttcagca aatttaatgc ggtacagcac cggatcgcgc atcacgataa
    22741 acggtgaagc catgtcgatt ttcgcacgca ggcaggcttt accttcttca aaaccaccgg
    22801 cacgcatttt ttcgaacagc gccaggttct cttcaacgct gcggtcgcgg tacgggctgt
    22861 ttttacccgg ttgcgtcagg gtgccgcggt attcgcggat ctgttccggc gtcagttcat
    22921 caacgtacgc caggccttta ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat
    22981 aatcggagga gtaacggacg ttaccagacc agtgaaaacc taaccactct acgtcgtttt
    23041 tgatcgactc aacatactcg atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt
    23101 tgcactggcc tttatagtcc tgggcgatcc cgaagttcag gcagatagat ttcgcatggc
    23161 caatatgcag atagccattc ggctccggcg ggaaacgggt gtgtactgtg gtgtgcttac
    23221 cactggccag atcttcatcg atgatctgac ggataaagtt agtcgggcgg gcttctgcct
    23281 cactcatcgt ggattcctca aagcgtaaac aacgtataac ggcgtatgat cttataagcg
    23341 ggacaggctg acaactgtta gttttttaaa attgataatc taacaaggat tatggggatg
    23401 attgatgaaa aaaaaagcgg cagagaagat ctccaccgct tgtcattgtt ggatgcgacg
    23461 ctcaagcgtc gcatcaggca taaagcagat tactttttga tttcatacag cggtgtttga
    23521 cccgccacaa catggccctg agctttaatg atcaagccac tgaaatcgtc gatattgctg
    23581 caaaccaccg ggctaatcat cgagcgggcg ttagcgttca ggtaatccag atccatttcc
    23641 agaatcggtt gccctgcgct tacctgcgca ccctcttcca ccagacgttt aaagccttta
    23701 ccttccagcg ctacggtgtc gatacccata tggacgacga tctccgcgcc tttttcggtt
    23761 tccaggcaga acgcgtggtt ggtgttgaag attttcacga ttgtccctgc ggctggtgat
    23821 acgacgattt tatctgtcgg tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat
    23881 gcttcgtcag gaacctgatc cagtgccacg acatcaccgg taatcggcga taccagctcc
    23941 gcgatagata ccgcgtttgg tacagcctgc ggttttgcta caggcgcggc agttgacgga
    24001 gttgcttcag ctgacgcagc ggctaccgga ccacgggcaa cgactttctt catcgcatcg
    24061 ccgatggatt ctgctttcgc gccaacaatc acctgaatag tctgtttgtt cagtttcact
    24121 acaccagaag cacccagacg tttacacatc gtatcgttga cgcgggcaga gtcagccacg
    24181 gtcaggcgca gacgggtgat acaggcgtca attgctttca ggttgtcagt gccgccaacc
    24241 gcagcaatat agttggtcgc cagttgattg aggccttctt cagtgttgct gttggcttct
    24301 tcagtaacga tctcgtcttc tttatcttca cgacccggcg ttttcaggtt gaacatgcgg
    24361 ataaccaaac tgaacaccac gaagtagata gcgaagaaga caacacccat caccagcagc
    24421 atccagacgt tctggctggc ggccggcagg ttatacatca acgcgtagtc gatagccccc
    24481 gcagagaaag agaagccagc atggataccc agcagtgttg ccacaaacag gctgataccg
    24541 gtcagcagtg cgtgcaggag gtacagcagc ggtgcaagga acatgaacag gaattccagc
    24601 ggctcagtca caccggtcag gaacgcagta acagcaacag aaagcagcat cccgccaacc
    24661 atcggacgac gctctttcgg tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg
    24721 aacatcataa tcgggaagaa gccggacatg aacatccccg cggtgccgtc accggcatag
    24781 aagcggttga tatcaccgtg gaaaaccgta cccgccgcgt tggtgaattc accaatctgg
    24841 aaccaggcga tggtgttcag cacctgatgc agaccggttg ggatcagcag acggttgatg
    24901 aaaccaaaga taccggaacc cagcgcgccc gcagaaacga tccactcgcc tcctgcatgg
    24961 atagcgtgct gtaccggcgg ccagacgtaa ccaaaaatgg ccgccagcac caggcagaag
    25021 aagccggtgg caatcggcac aaaacgtttg ccgccgaaga agctcaggaa gtccggcagt
    25081 ttaatatcgg accaacggtt ataggctgcg ccaccaacca gaccggtaat gatacccgcc
    25141 agtacaccca tgtttatttc tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa
    25201 cctaccgcac ccgccagcgc cgccgcacct gcgctgtctt tcgaccagct ggatgccaca
    25261 ccgatggcga agattaatgc gaggttatca aaaatcgcac cgcccgcctg ggcaataaac
    25321 gcaacgttaa gtaaatctgg ctgaccgaat cgcagcaaca gtgccgccac cggcagcacc
    25381 gcgataggga gctgtaacgc cctaccgagt cgctggaaaa aacctaaaat attcatctta
    25441 ttccccctac gagaacccta tttggctcgt ttaaagccgt atttttattt tgctgcaaat
    25501 tgtactgccg atgttctgta atcagattgt tagatcatct gctacagagt gtgtgaaaat
    25561 ttaattcgta tcgcaaatta aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta
    25621 tcatcactcc cttttactgg ctaaaccaga aaacttattt tatcattcaa aaaatcaggt
    25681 cggattgacg cctgtctgcg caaatccaag ttacgcttaa agatacctaa tccgccaacg
    25741 gcttacattt tacttattga ggtgaataat gagactgatc cccctgacta ccgctgaaca
    25801 ggtcggcaaa tgggctgctc gccatatcgt caatcgtatc aatgcgttca aaccgactgc
    25861 cgatcgtccg tttgttctgg gcctgccaac tggcggcacg ccgatgacca cctataaagc
    25921 gttagtcgaa atgcataaag caggccaggt cagctttaag cacgttgtca ccttcaacat
    25981 ggacgaatat gtcggtctgc cgaaagagca tccggaaagc tactacagct ttatgcaccg
    26041 taatttcttc gatcacgttg atattccagc agaaaacatc aaccttctca acggcaacgc
    26101 cccggatatc gacgccgaat gccgccagta tgaagaaaaa atccgttctt acggaaaaat
    26161 tcatctgttt atgggcggtg taggtaacga cggccatatt gcatttaacg aaccggcgtc
    26221 ttctctggct tctcgtactc gtatcaaaac cctgactcat gacactcgcg tcgcgaactc
    26281 tcgtttcttt gataacgatg tcaatcaggt gccaaaatat gccctgactg tcggtgttgg
    26341 tacattgctg gatgccgaag aagtgatgat tctggtgctg ggtagccaga aagcactggc
    26401 actgcaggct gcagttgaag gttgcgtgaa ccacatgtgg accatcagct gtctgcaact
    26461 gcatccaaaa gcgatcatgg tgtgcgatga accttccacc atggagctga aagttaagac
    26521 tttaagatat ttcaatgaat tagaagcaga aaatatcaaa ggtctgtaat tgttatccct
    26581 gccctggctc cttgctcagg gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta
    26641 acccagggcc ggatctttac cggccacgaa tttcttgatg accacgcggt tgttatcgct
    26701 gatggcctga ttaaaagcgt ctgtccggta gcggaactgc cgccagagat cgaacaacgt
    26761 tcactgaacg gggccattct ctcccccggt tttatcgatg tgcagttaaa cggctgcggc
    26821 ggcgtacagt ttaacgacac cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag
    26881 aaagccaatg agaaatcagg ctgtactaac tatctgccga cgcttatcac caccagcgat
    26941 gagctgatga aacagggcgt gcgcgttatg cgcgagtacc tggcaagaca tccgaatcag
    27001 gcgttaggtc tgcatctgga aggtccgtgg ctgaatctgg taaaaaaagg cacccataat
    27061 ccgaattttg tgcgtaagcc tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac
    27121 gtcattacca aagtgaccct ggcaccggaa atggttcctg cggaagtcat cagcaaactg
    27181 gcaaatgccg ggattgtggt ttctgccggt cactccaacg cgacgttgaa agaagcgaaa
    27241 gccggtttcc gcgcggggat tacctttgcc acccatctgt acaacgcgat gccgtatatt
    27301 accggtcgtg aaccgggcct ggcgggcgcg atcctcgacg aagctgacat ttattgcggt
    27361 attatcgctg atggcctgca tgttgattac gccaacattc gtaacgctaa acgccttaaa
    27421 ggcgacaaac tgtgtctggt taccgacgcc accgcgccag caggtgccaa cattgaacag
    27481 ttcatttttg cgggtaaaac aatatactac cgtaacggac tttgtgtgga tgagaacggt
    27541 acgttaagcg gttcatcctt aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc
    27601 ggtatcgcac tggatgaagt gctgcgtatg gcgacgctct atccggcgcg tgcgattggc
    27661 gttgagaaac gtctcggcac actcgccgca ggtaaagtag ccaacctgac cgcattcaca
    27721 cctgatttta aaatcaccaa gaccatcgtt aacggtaacg aggtcgtaac tcaataagag
    27781 aaagtatgac accaggcgga caagctcaga taggtaatgt tgatctcgta aaacagctta
    27841 acagcgcggc ggtttatcgc ctgattgacc agtacgggcc aatctcgcgg attcagattg
    27901 ccgagcaaag ccagcttgcc cccgccagcg taaccaaaat tacgcgtcag cttatcgaac
    27961 gcgggctgat caaagaagtt gatcagcagg cctccaccgg gggccgccgc gctatctcca
    28021 tcgtcaccga aacccgcaat ttccacgcaa tcggcgtacg gcttggtcgt cacgacgcca
    28081 ccatcaccct gtttgatctc agcagcaaag tgctggcaga agaacattac ccgctgccgg
    28141 aacgtaccca gcaaacgctg gaacatgccc tgttgaatgc cattgctcag tttattgata
    28201 gctaccagcg caaactgcgc gagctgatcg cgatttcggt gatcctgcca gggcttgttg
    28261 acccggacag cggcaaaatt cattacatgc cgcatattca ggtagaaaac tgggggctgg
    28321 tagaagctct ggaagaacgt tttaaagtga cctgtttcgt tggtcacgat atccgtagtc
    28381 tggcgctggc ggagcactac ttcggtgcaa gtcaggattg cgaagactcc attctggtgc
    28441 gtgtccatcg cggaaccggg gccgggatta tctctaacgg gcgcattttt attggccgca
    28501 acggtaacgt cggtgaaatt ggccatattc aggtcgaacc gctgggtgaa cgctgccact
    28561 gcggcaactt tggctgcctg gaaactatcg ccgccaacgc tgccattgaa caacgggtgt
    28621 tgaatctgtt aaagcagggc taccagagcc gcgtgccgct ggacgactgc accatcaaaa
    28681 ctatctgcaa agccgcgaac aaaggcgata gtctggcgtc ggaagtaatt gagtatgtcg
    28741 gtcgtcatct gggtaaaacc atcgccattg ctatcaactt atttaatccg caaaaaattg
    28801 ttattgccgg tgaaatcacc gaagccgata aagtgctgct ccctgctatt gaaagctgca
    28861 ttaataccca ggcgctgaag gcgtttcgca ctaatctgcc ggtggtacgt tctgagctgg
    28921 atcaccgctc ggcaatcggc gcttttgcgc tggtaaaacg cgccatgctc aacggtattt
    28981 tgctccagca tttgctggaa aattaatgtg cttttatagt ggcgcttatt gttgtcaata
    29041 ttctgggtag tccatgacca ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca
    29101 cgataacgtc gccgtaccgg gtgcagcgga atttttgcac gggattatgg ataaaggcct
    29161 gccgctggtg ttgctgacca actatccttc gcagactggg caagatctgg cgaaccgctt
    29221 tgccaccgca ggtgtcgatg tacctgacag cgtgttttat acctctgcga tggcgactgc
    29281 cgatttcctg cgtcgccagg aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat
    29341 tcatgaactg tacaaagccg gtttcactat taccgatgtg aaccctgatt tcgtgattgt
    29401 tggcgaaacg cgttcctaca actgggacat gatgcataaa gcagcctatt tcgtcgctaa
    29461 cggtgcacgt tttatcgcca ccaatccgga cacccacggg cgcggttttt atcccgcgtg
    29521 tggcgcgttg tgtgcaggca tcgaaaaaat ctccgggcgt aaaccgttct atgttggtaa
    29581 accgagtccg tggatcatcc gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac
    29641 ggttattgtc ggcgataacc tgcgtaccga tattctggcg ggcttccaag caggtctgga
    29701 aacgattctg gtgctttctg gtgtttcatc gctcgacgat atcgacagta tgccgttccg
    29761 cccaagctgg atttacccgt cggtcgctga aatcgacgtt atctgaatag caaccacgtc
    29821 tacagacgtg gttggctctg atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag
    29881 gcctacgcag ctcctgcaat atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt
    29941 cacgccgcat ccggcatgaa caaattgcac tttgtcagca atttggcccc cgtaaaatat
    30001 aaacattcaa taaaacccgc taaaaattat cgattcatca ataaatatat cctcacacca
    30061 gacctttttt cagcaatcga caatcaccat tacgtttttt attttttcac cgccaaatcg
    30121 cttgcgcaca gtgctgcttt gcggcatttt tttaaacaag caaacacaac aagcaacaaa
    30181 taccaggtta acggagaagg ttatgtgttc aatttttggc gtattcgata tcaaaacaga
    30241 cgcagttgag ctgcgtaaga aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc
    30301 ggactggtcc ggtatttatg ccagcgataa cgccattctc gctcacgaac gtctgtcaat
    30361 tgttgacgtt aacgcggggg cgcaacctct ctacaaccaa caaaaaaccc acgtactggc
    30421 ggtaaacggt gaaatctaca accaccaggc attgcgcgct gaatatggcg atcgttatca
    30481 gttccagaca gggtctgact gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga
    30541 atttctcgac gacttgcagg gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc
    30601 ctacctgatt ggtcgcgacc atctggggat catcccactg tatatgggct atgacgaaca
    30661 cggtcagctg tatgtcgcct cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa
    30721 agagttcccg gcggggagct atttgtggag ccaggacggc gaaatccgtt cttattatca
    30781 tcgcgactgg ttcgactttg atgcagtgaa agataacgtg accgacaaaa acgagctgcg
    30841 tcaggctctg gaagattccg ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct
    30901 gctttctggt ggtctggatt cctcaattat ttccgctatc accaagaaat acgcagcccg
    30961 tcgcgtggaa gatcaggaac gctctgaagc ctggtggccg cagttacact cctttgctgt
    31021 aggtctgccg ggttcaccgg atcttaaagc agcccaggaa gtggcaaacc atctgggcac
    31081 ggtgcatcac gaaattcact tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat
    31141 ttaccacatc gaaacttatg atgtgaccac tattcgcgct tcaacaccga tgtatttaat
    31201 gtcgcgtaag atcaaggcga tgggcattaa aatggtgctg tccggtgaag gttctgatga
    31261 agtgtttggc ggttatcttt acttccataa agcgcccaac gctaaagaac tgcatgaaga
    31321 gacggtgcgt aaactgctgg ccctgcatat gtatgactgc gcgcgcgcca acaaagcgat
    31381 gtcagcctgg ggcgtggaag cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc
    31441 gatgcgcatt aacccgcagg ataaaatgtg cggtaacggc aaaatggaaa aaaacatcct
    31501 gcgtgaatgt tttgagtcat acctgcccgc aagcgtggcc tggcggcaga aagagcagtt
    31561 ctccgatggc gtcggttaca gttggatcga caccctgaaa gaagtggcgg cgcagcaggt
    31621 ttctaatcag caactggaaa ctgcccgctt ccgcttcccg tacaacacgc caacctcaaa
    31681 agaagcgtat ctgtaccggg agatctttga agaactgttc ccgcttccga gcgccgctga
    31741 gtgcgtgcct ggcggtcctt ccgtcgcgtg ttcttccgct aaagcgatcg aatgggatga
    31801 agcgttcaag aaaatggacg atccatctgg tcgtgcggtt ggtgttcacc agtcggcata
    31861 taagtaagaa atgcgaaaca gccccggtga aatccggggc tgtttcagtt attgaggtgc
    31921 ttgttgattc tgatatgcag taaggaaatg ttccgggttt gccacatcac tgtttgacaa
    31981 ctcatcactg taggccagta tttcatagat taatttccca ccactattgt tgaattccga
    32041 atttcatcct gtacgacaaa agaatggctg tagggtgggg aaaaaacaca aagtaaacaa
    32101 taattgacga atatagcgcc acgctgttcg caacctaacc aaacagtcac tttcgagcaa
    32161 ttttccttga aaaagaggtt gacgctgcaa ggctctatac gcataatgcg ccccgcaacg
    32221 ccgataaggt atcgcgaaaa aaaagatggc tacgtagctc agttggttag agcacatcac
    32281 tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc accatctttt tttgcgggag
    32341 tggcgaaatt ggtagacgca ccagatttag gttctggcgc cgcaaggtgt gcgagttcaa
    32401 gtctcgcctc ccgcaccatt caccagaaag cgttgtacgg atggggtatc gccaagcggt
    32461 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatctt
    32521 cttcgagtaa gcggttcacc gcccggttat tggggtatcg ccaagcggta aggcaccggt
    32581 ttttgatacc ggcattccct ggttcgaatc caggtacccc agccatagaa gaaacaatct
    32641 ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca caggttcgaa
    32701 tcccgtcgta gccaccaaat tctgaatgtg tcgaatacgt tcggcaaatt caaaaaccaa
    32761 tttgttgggg tatcgccaag cggtaaggca ccggattctg attccggcat tccgaggttc
    32821 gaaccctcgt accccagcca atttattcaa gacgcttaca tcgtaagtgc acccagttgg
    32881 ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc
    32941 gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc tgcgttcatt
    33001 caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga cgctgccgcg
    33061 tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca accctaacgc
    33121 atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta acccgagatt
    33181 acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca gatccatacc
    33241 actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga ggacaggata
    33301 actggcccac gcttcaccgt aactgcgggc attcaccaga acatcaatca gggcatcgac
    33361 atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat gcgcggcatc
    33421 gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg tcagcggaaa
    33481 cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gagaaatgct tcgcgatttc
    33541 cgcctggagt tgcgccatat tcatattctg caactggcga atacgcgccg gagagtcata
    33601 ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc cgtccggggt
    33661 aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa tcaacatgca
    33721 cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca cctgagactt
    33781 tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct tcagttccag
    33841 ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac gtaacgttac
    33901 tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacggtgt tttccaccat
    33961 atagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg ccgtttccca
    34021 ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg catcccagac
    34081 ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc gcacgtccgg
    34141 ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta ccgaaaatcc
    34201 gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt cctccgccga caatggcaat
    34261 ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt aagtttacag
    34321 gatttttacc cccaagggct gataacgctc atactggcca caacggcagc aaagcattac
    34381 actatgcgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga ccaaaaaact
    34441 ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga tggccgatct
    34501 gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg atgtgctgct
    34561 gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc agttggggcg
    34621 ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg gctgcgtggc
    34681 atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta tttttgggcc
    34741 gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc gcagcccggt
    34801 tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac cgcgcgccga
    34861 agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca cctactgcgt
    34921 ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc tgtttgagat
    34981 tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga acgtgaacgc
    35041 ctggcgtggt gaaaactacg acggctccac cggatcgttt gccgatctgc tgcgtctggt
    35101 tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga tcgaattcac
    35161 cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc tgcatctgcc
    35221 ggtacagagc ggttccgatc gcattctgaa cctgatgggc cgtactcata cggcactgga
    35281 gtacaaagcg atcatccgta aactgcgtgt ggcgcgtcca gatattcaga tcagttctga
    35341 tttcatcgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga tgaagctgat
    35401 tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc cgggtacacc
    35461 agccgccgat atggttgatg atgttccgga agaagagaag aagcagcgtc tgtatattct
    35521 gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg gcaccaccca
    35581 gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg ggcgtaccga
    35641 aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat tcgtcgatgt
    35701 agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta ctgaagatga
    35761 aatgggcctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc gcaaagaaaa
    35821 cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc ggtgggcctt
    35881 gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc ctgtggcgtg
    35941 aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga taagaggaac
    36001 ggtttgaaca tagacactcg cgaaatcacc ctagagccag cagacaacgc gcgtctgttg
    36061 agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct cggcatcgag
    36121 atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt caccgctgcg
    36181 gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca gattcaggat
    36241 atcgaaccgg aacagatcca ccttacgatt aaagaagcgc gagtactgga gcaaagtgcg
    36301 gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg cgtaattaag
    36361 ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga cattaccttc
    36421 ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc agttgatgcc
    36481 ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga agccggtgag
    36541 aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct gcgcccactg
    36601 tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat tgagcgcaac
    36661 gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga cgcgtttatc
    36721 attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct gacccgtatc
    36781 ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct gccgcgtaat
    36841 actaaatcag gcttacgtca cgccatcgaa gtactggccg atgtcgaaga gatcagcttt
    36901 aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat cgttaacgcc
    36961 tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcag cgctggcggc agaacgcaaa
    37021 cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg gcatgtgaag
    37081 ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg gtgatcccac
    37141 agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa agccacagtc
    37201 tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc ccgtttgaag
    37261 tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt caggtggttg
    37321 agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat atggtggtgc
    37381 acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca gaagaaatgg
    37441 aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac attgccgaga
    37501 aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga ttaacgacgg
    37561 cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca attcacacag
    37621 tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc aacttttcca
    37681 cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg ggcagaacga
    37741 ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg cagaccaacg
    37801 cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca accagacgct
    37861 ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg tgattagcga
    37921 agacaaagat cacattgaag ggattctgat ggcaaaagac ttgctgccgt ttatgcgcag
    37981 cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg ttcctgaaag
    38041 taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca tggcgatcgt
    38101 cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc tggaactgat
    38161 tgttggtgag attgaagacg aatatgacga agaagatgat atcgacttcc gtcagctgag
    38221 tcgtcatacc tggaccgtgc gcgcactggc ttccattgaa gacttcaacg aagcgttcgg
    38281 cacccacttt agcgatgaag aagtcgacac tatcggtggt ctggtgatgc aggcgtttgg
    38341 gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca aagtggcgat
    38401 ggccgacagt cgacgtatta ttcaggttca tgtcaaaatc ccggatgact caccccagcc
    38461 gaagctggat gaataaaacc gaaactggat agataactac atggcttttg cctcattaat
    38521 tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg gaacgctggc
    38581 cttctctcct tacgacgtct ggcctgcggc gattatttcg ttgatggggc ttcaggcgtt
    38641 gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg gatttggcct
    38701 ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg gaatgcctgg
    38761 cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt ataccggact
    38821 gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg tggcgattgc
    38881 cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga caggcttccc
    38941 gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg caccgataat
    39001 gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg cactggcgtt
    39061 ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc tacccttccc
    39121 gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg tttcgatggt
    39181 tcagggcgat attccgcaat cactgaaatg ggatggagat cagcttctta atacgctgaa
    39241 gatttactac aacgcaacgg ctccgctgat gggcaaatca tcgttgatta tctggccgga
    39301 gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac tggacggtga
    39361 gttgcgcgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc tcaataagca
    39421 gaaccgctac gatacctaca acaccatcat cacactgggt aaaggtgcac cgtacagcta
    39481 cgaatcagcc gatcgctata acaaaaacca tctggtgccg tttggcgagt ttgtcccgct
    39541 ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt cgttcagccg
    39601 tgggccatat atccagccgc cgctgtcagt aaatggtatt gagcttactg cggctatttg
    39661 ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata ccgactatct
    39721 gctgactatc tccaacgatg cgtggtttgg taaatctatt ggtccatggc aacacttcca
    39781 gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca ccaacaacgg
    39841 cattacggcg gtgattggcc cgcagggtga gattcaggcg atgatcccgc agttcacccg
    39901 cgaggtgtta accactaatg tgacaccgac caccggactc acaccgtatg cacgtaccgg
    39961 caactggcca ctgtgggtgc tgacggcact gtttggtttt gccgctgtgt tgatgagtct
    40021 gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg tactttctca
    40081 ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga tacaagccac
    40141 attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg tcctgtccat
    40201 ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg aaaaacgaag
    40261 gtgattaacg tagctggcag gataaacgcc aataatctgc ccggcattaa tattgagcag
    40321 gatgacataa ccttgcaggc catatgacac caacgctaaa tattgaccgt ccgggctaat
    40381 accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg cttgttgatc
    40441 ccctacgctt atacgtgaaa tcccctgact atctgcaaaa ataacctcgc cgttttcagg
    40501 tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt cttctttctg
    40561 atgcgtgatg gtattgatcc gcacgatgtt acctctgcct gctcccggaa ccagtaatgt
    40621 tctttcatcc ggggaaagtg ccataaccca ggcgcctggt ttaccgctcg tccccgcccc
    40681 gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat caatcgctga
    40741 cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg agaggataag
    40801 gctgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccctgtcg ccacgttaat
    40861 gaccgataac gcattcgctt caaatgaagt cgcataaatc aacttttcat ccgggcttaa
    40921 tgtcagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg acttttcttt
    40981 cgctatgggc gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga aaacaacaat
    41041 gctgttcgtg ttttcaattg cggcatacaa catattacca tcccgactga tcgccatatc
    41101 atggatatcg gcaatctcag cgagcaaatc accactggcc tgttcacgac ttaccggagc
    41161 ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgctgctttt tttgccgtcg
    41221 ctcttgttca atctcctgtg gataaaacgc ttcacgcagg ctttgcctga cttgtaaaac
    41281 tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa gactgcccgc
    41341 aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg cgctcagcca
    41401 acccaacgcg ccacccgcaa tcaggccgcc aatgtgacaa gcattatcta ttcctgtttg
    41461 ccgggcacca tacaacagtg tcagcgccac catacccaac agagaaaata ctcgccgctg
    41521 gtttttatgg tatgtgcccg cacctgaagc aagctgtgtt gctatcgcag cgccagctat
    41581 tcccataatt gccccggatg ccccaacgct gatgactatc tgatcactat cgcgtaacgc
    41641 ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaggtaa tcaggagtcg
    41701 ccatttcccc agaatctgtt ccgccacacc tcccacgacc aggaatgcaa acatattcat
    41761 cagcagatga gaaacactac tgtgcagaaa gatactgctc aataagcgcc agggttcacc
    41821 cgacaaagta agaggcgcaa cgtttgcgcc gagatggata agtgcggatt gatcgacctc
    41881 ttcataactc atccattgtg cgagataaca aaatatccca atacagagtg caaaaaggat
    41941 aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag acataaggtg
    42001 tttcttcctt gaaaagacag gtaaaaagca tttttcccgt gtgcagttat catcccggca
    42061 aacgtcccat gccgaaaaga taattctgtc cgaaatatga ctttttcagc ctgaaattac
    42121 aatcgtaatt aatttgcccc taagtatttt ttggcatgta agttgcttta tttactgtgt
    42181 aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag cggtagcacc
    42241 aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg gcgcatctcg caaaaacaaa
    42301 caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac aggcacaaca
    42361 ctgcacaata aggttgcaga cgataacaac acaaacactc acaacgggta tccatgcgtt
    42421 cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca cagcaatcct
    42481 cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg cagcaggcag
    42541 tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg aatcttcagt
    42601 acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg attactccaa
    42661 cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg taaaactgat
    42721 tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg attttgaatg
    42781 tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg atactatttt
    42841 cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt ttgccgacct
    42901 gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc tcaacaaact
    42961 gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcacg gtgactcttt
    43021 ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc tgctggccgg
    43081 tgaacgtgcg aaagcgaaga aaccagacaa ctgggatatc gtcggcaagc cgcagtctca
    43141 ggaagcctac ggttgcatgt tgcgtaaaga tgatccgcag ttcaaaaagc tgatggatga
    43201 cactatcgct caggtgcaga cttccggtga agcggaaaaa tggtttgata aatggttcaa
    43261 aaatccaatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg aaatgaaagc
    43321 actgttcaaa gaaccgaatg acaaagcact gaactaatta caagaaccag gggcggaaat
    43381 tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg tcgttcccca
    43441 tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag ttaacgctgc
    43501 taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt atttttttac
    43561 aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt tttcaggtga
    43621 cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca tttttcggca
    43681 ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat gtcgaactgt
    43741 tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc ccggagctgt
    43801 tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt cagtttttcc
    43861 tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa caggtacgcg
    43921 ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg ggcctgacgc
    43981 taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc gtcccgccga
    44041 tgacttccga gatgatgaac ctggtgaaaa actccgtcat cgcctcgact attggtctgg
    44101 tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc tgggagtcgt
    44161 ttaccgctat tacgctggcg tatgtgttga ttaacgcgat tatcatgctg gtaatgacgc
    44221 tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt acgagtttga
    44281 ctggagttcc attgtccctt ccctgccata ttagctcgac gggctggtga tcacgctgaa
    44341 aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg cggtgatgcg
    44401 cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg tattccgctc
    44461 gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt ttctgcaaaa
    44521 cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg tggcgttttc
    44581 gatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa gtatttctcg
    44641 tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga tgaaactgat
    44701 tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg gcatcgtact
    44761 gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc gtaccgcctc
    44821 aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctgtttgccg gatttgttta
    44881 tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa ggacagcata
    44941 atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct gaccgactgc
    45001 tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg ttccggcaaa
    45061 tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga aatcaccgtc
    45121 gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc ccgcgtcggg
    45181 atggtgttcc agcattttga gttgttcccc catttgtcga ttattgaaaa cctgactctg
    45241 gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc attgaaattg
    45301 ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct ttccggcggt
    45361 cagcagcagc gtgtggcaat cgctcgcgcg ttgtgtatgg atcctattgc gatgctgttc
    45421 gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga cgtgatggtg
    45481 gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg ctttgcccgc
    45541 aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga ctcgccgaaa
    45601 gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag acttcctcgc gaaaatcctg
    45661 cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta tcgtttcgcc
    45721 cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca acaaggagca
    45781 acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc tatcgcaata
    45841 gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc cgccggaaac
    45901 cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct taatcgcacc
    45961 gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat tatcgcggac
    46021 aatgtgcacg gcgaaagcgg tctggatggc ccggcattac cggaaccgac gttcgcaccg
    46081 caaaactgta cggcggtaga gctgatggcg aaaacgctgc gcgaaagtgc ggaacctgtc
    46141 acgattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag ccatccggaa
    46201 ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct tggtaactgg
    46261 acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat tgtcttccag
    46321 tcagggatcc cagtggtgat ggccggtctg gatgttactc ataaagcgca aatccacatt
    46381 gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt tgccgaactg
    46441 ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg tgcaccgctg
    46501 catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac tgttgagcgc
    46561 tgggttggcg tggaaacaca ggggaaatat acccagggta tgacggttgt tgattattat
    46621 tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg tcagggcttt
    46681 gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt gattaagatc
    46741 accacgcgtt acctgggtgg tcttaatctg tttatctctt tttctttcaa gatataaatg
    46801 caatggataa tgcagaactc accattggta tcgatctcgg cactaccaat agtttaattg
    46861 ccgtctggaa agacggtgcc gcgcaattaa ttccaaataa gttcggtgaa tatttaacac
    46921 catccataat tagcatggat gaaaataatc atattttagt cggaaaaccg gctgtatcac
    46981 ggcgtacttc gcatccggat aaaacggcag cgttatttaa acgcgcaatg ggcagtaata
    47041 ccaactggcg gttaggcagc gacacattta acgcgccaga actgtcctct ttggtattac
    47101 gctcattaaa agaagatgcc gaagaatttc tgcaacgtcc gattaaagat gtggtgatct
    47161 ccgttccggc ttatttcagc gatgaacaac gcaagcatac ccgtttagct gcggagttag
    47221 ccgggttaaa tgcggtacgc ttaattaatg aacccacagc agctgcgatg gcatatggcc
    47281 tgcatacgca acaaaatacc cgctcgctgg tttttgatct cggtggcggc acgtttgacg
    47341 ttacggtgct tgagtacgcc acgccggtga ttgaagttca cgcctccgct ggcgatagct
    47401 tcctcggtgg cgaagatttt acccatatgc tggtcgatga ggttttaaaa cgcgcggaag
    47461 ttgccaggac cacgcttaac gagagtgaac tggcagcctt gtacgcctgt gtggaagcgg
    47521 caaaatgtag caatcaatcg ccattgcaca ttcgctggca gtatcaggaa gaaacgcggg
    47581 aatgcgaatt ttacgagaac gaactggaag atttgtggtt gccgctgctc aatcgcttgc
    47641 gagtgccgat tgaacaggcg ttgcgcgatg cgcgtctgaa gccgagtcaa atcgacagtc
    47701 tggtgctggt tggcggcgcg tcacaaatgc cgctggtgca gcgaatcgcc gtgcgtctgt
    47761 ttggcaaatt accgtatcaa agttacgatc cgagcaccat tgtcgcgctg ggcgcagcaa
    47821 tccaggccgc ctgccgctta cgcagtgaag atattgaaga ggtaatcctc actgatattt
    47881 gcccttactc gttgggcgtt gaagttaacc gccagggcgt ttccggcatt ttctcgccga
    47941 ttattgaacg aaacaccaca gtaccggtgt cgcgtgtaga aacttactcg accatgcacc
    48001 cggagcagga ttcaattacg gttaacgtct atcagggaga aaaccacaaa gttaaaaata
    48061 acattctggt tgaatccttc gatgtgccgt tgaagaaaac cggggcttat cagtcgattg
    48121 atattcgctt tagttatgat atcaacgggt tgcttgaagt tgacgtgctt ctgaaagacg
    48181 gcagcgttaa gtccagagtg attaaccaca gcccggtaac attgagcgcg cagcagattg
    48241 aagagagtcg gacgcggtta tccgcattga aaatttatcc gcgcgatatg ctcatcaatc
    48301 gcacctttaa agccaaactg gaagagttgt gggcgcgagc gctgggtgac gagcgagaag
    48361 agattggccg ggtgatcacc gattttgatg cggcgctaca gtcaaacgac atggcccgcg
    48421 tggatgaagt tcggcggcgg gcgagcgatt atttagccat tgagatccca ttaacacggc
    48481 tgccggatgc ggcgtaaacg ctgcatccgg catcggcgca aaaaacacat cgtcaactaa
    48541 acaacatcgc gattttgccg agagcataac ctatcaccag caccgttaaa ctgattttga
    48601 taatgctggt aatgctctgt ggtttttcat cctcatcatt tttttcttca tggttttcag
    48661 gtgttggctc atcgacatct tcatcctccg gctcgagttt cactttcgtc aacagcatcc
    48721 gggtgataat ggcctctaat ggatgagcat ctccgccttc cagccttgta ttaagacggg
    48781 ccagcatctc ttgtttcgga aacgcttcaa gcactacctg ttccgctacc cgatacaaat
    48841 ccagtaacgg tgaatcttgc tctgtctcgc taccgactct cgatttccac cacgcccatg
    48901 ctttgcgttt gaagaataac gactcaataa acgattgacc taacggcgat aattttccgc
    48961 tatgtaaaat ctccgcccat aacagccgcg tccgggcgct ctcaccagga acatatttca
    49021 atggtttacc ttgcgcatct accatgctct gcgcttcgat aaccatcact aataacgcga
    49081 aataatctgg gcaatgacgc ttactccact gcaacaacaa attatcagct tgtgtagatg
    49141 ggtaactttc ccggtacgcg cagagatcgg caagcaggct ttccccctcg ccgcagtaca
    49201 acctttgagc acacaagtaa tatcgggcat cttcgctttc tggttcttcc atttgccact
    49261 gtttggctac aggaatgagt tccgccatac ctgtttgcac cgagctgtac cagcgcaaca
    49321 gtttgcgatg taactttgca tcatcaggaa tgaaccacgg accttccatc gccaaaaacg
    49381 cagtgacata gtcaggatgg taacggcagc aacgttccag cgcgaagtaa tactcaacag
    49441 tctggttctg caacgcaacg ggatattcca gaaggctaag gaaatcgaaa caatcaccag
    49501 cctgaatggc ctcaagaaaa gcctcccttt cttcctcatc cattccttcg ctgtcattaa
    49561 acgaatgcca gttcaaacgc tctgccagca aagaagcaca attcaatgaa cgatatcgcg
    49621 cttctatagc gattgcacac agcggccagc gtaattgatc gacatcctcc atgttccagg
    49681 tatttaattg ctgaataaat ttctgctagg cggagggatg aaaacgatca ctttctgaat
    49741 ccagtaatgt cctgaatgca cgcagtattt catgttcagc ggcggcatct ttttcttcat
    49801 catctgcttc ctcgacagga tttaccgcca gtcgcagcgc ctcttcatag gcctggcgta
    49861 atgctttaac cccctgcgga tcggtttcgg gatgacataa cggtaagcga gcaagataag
    49921 cctggcggat aatgtctatt tgcgtcgtgc tttcaatttc gaggatttgc caacatgttt
    49981 tcataggtcg ctcacatcct taagcgttac cgggcattca ggctggacag ttaatggttg
    50041 ccagggaagc gtggcataga aacgatcgct tcccgctgcc agctcttcga acatccgact
    50101 cccggcgcta tcgtatttgt acagggttag cgcctcgcgt agcgaagtgt tgtcttcgca
    50161 ctgagaagat tgccagtaga gacgccctaa cgaataggca gaaaaatagt gcatccagcc
    50221 gtgatagtaa tggtgagctc gtacatagac gcgcgattgc agccataaac tctcttcttc
    50281 cgtcagccac ttgttgagca caccgacccg tgacagaacc cccattcgaa cataatccca
    50341 ggctttgatt cc
//
LOCUS       DAJTNA010000007        49855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTNA010000007 DAJTNA010000000
VERSION     DAJTNA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49855
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.007"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..504
                     /locus_tag="OO161_000328"
     CDS             <1..504
                     /locus_tag="OO161_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5461517.1"
                     /translation="NYALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGL
                     KTDWRGYTVVPYATSYRENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAG
                     ARVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPD
                     QICHISY"
     gene            552..1409
                     /locus_tag="OO161_000329"
     CDS             552..1409
                     /locus_tag="OO161_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5461518.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDIDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYP"
     gene            1440..1622
                     /locus_tag="OO161_000330"
     CDS             1440..1622
                     /locus_tag="OO161_000330"
                     /inference="COORDINATES: protein motif:HMM:NF012635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5461519.1"
                     /translation="MVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVSTTGVPP
                     ETGRFEATATVRINFD"
     gene            1634..2176
                     /locus_tag="OO161_000331"
     CDS             1634..2176
                     /locus_tag="OO161_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5461520.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVIYFDLVYQ"
     gene            2184..2699
                     /locus_tag="OO161_000332"
     CDS             2184..2699
                     /locus_tag="OO161_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5461521.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            2665..3402
                     /locus_tag="OO161_000333"
     CDS             2665..3402
                     /locus_tag="OO161_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5461522.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            3513..4523
                     /gene="pyrD"
                     /locus_tag="OO161_000334"
     CDS             3513..4523
                     /gene="pyrD"
                     /locus_tag="OO161_000334"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5461523.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKDKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            4697..5239
                     /gene="zapC"
                     /locus_tag="OO161_000335"
     CDS             4697..5239
                     /gene="zapC"
                     /locus_tag="OO161_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5461524.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SSAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(5236..6345)
                     /gene="ycbX"
                     /locus_tag="OO161_000336"
     CDS             complement(5236..6345)
                     /gene="ycbX"
                     /locus_tag="OO161_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5461525.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            6589..8697
                     /gene="rlmKL"
                     /locus_tag="OO161_000337"
     CDS             6589..8697
                     /gene="rlmKL"
                     /locus_tag="OO161_000337"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5461526.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSRGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            8709..10616
                     /locus_tag="OO161_000338"
     CDS             8709..10616
                     /locus_tag="OO161_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5461527.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVGEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPYKTVMDNIAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQLAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            10746..11999
                     /gene="pqiA"
                     /locus_tag="OO161_000339"
     CDS             10746..11999
                     /gene="pqiA"
                     /locus_tag="OO161_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5461528.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            12004..13644
                     /gene="pqiB"
                     /locus_tag="OO161_000340"
     CDS             12004..13644
                     /gene="pqiB"
                     /locus_tag="OO161_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5461529.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            13641..14204
                     /gene="pqiC"
                     /locus_tag="OO161_000341"
     CDS             13641..14204
                     /gene="pqiC"
                     /locus_tag="OO161_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5461530.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNILVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            14460..14627
                     /gene="rmf"
                     /locus_tag="OO161_000342"
     CDS             14460..14627
                     /gene="rmf"
                     /locus_tag="OO161_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5461531.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(14697..15215)
                     /gene="fabA"
                     /locus_tag="OO161_000343"
     CDS             complement(14697..15215)
                     /gene="fabA"
                     /locus_tag="OO161_000343"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5461532.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(15284..17044)
                     /locus_tag="OO161_000344"
     CDS             complement(15284..17044)
                     /locus_tag="OO161_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5461533.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            17230..17682
                     /gene="matP"
                     /locus_tag="OO161_000345"
     CDS             17230..17682
                     /gene="matP"
                     /locus_tag="OO161_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5461534.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(17758..18798)
                     /gene="ompA"
                     /locus_tag="OO161_000346"
     CDS             complement(17758..18798)
                     /gene="ompA"
                     /locus_tag="OO161_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5461535.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDGLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(19155..19664)
                     /gene="sulA"
                     /locus_tag="OO161_000347"
     CDS             complement(19155..19664)
                     /gene="sulA"
                     /locus_tag="OO161_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5461536.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            19883..20512
                     /gene="sxy"
                     /locus_tag="OO161_000348"
     CDS             19883..20512
                     /gene="sxy"
                     /locus_tag="OO161_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5461537.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(20475..22637)
                     /gene="yccS"
                     /locus_tag="OO161_000349"
     CDS             complement(20475..22637)
                     /gene="yccS"
                     /locus_tag="OO161_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5461538.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(22647..23093)
                     /locus_tag="OO161_000350"
     CDS             complement(22647..23093)
                     /locus_tag="OO161_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5461539.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            23216..25270
                     /gene="helD"
                     /locus_tag="OO161_000351"
     CDS             23216..25270
                     /gene="helD"
                     /locus_tag="OO161_000351"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5461540.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNKLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(25302..25760)
                     /gene="mgsA"
                     /locus_tag="OO161_000352"
     CDS             complement(25302..25760)
                     /gene="mgsA"
                     /locus_tag="OO161_000352"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5461541.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(25856..26518)
                     /locus_tag="OO161_000353"
     CDS             complement(25856..26518)
                     /locus_tag="OO161_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5461542.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSKLDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            26691..27104
                     /locus_tag="OO161_000354"
     CDS             26691..27104
                     /locus_tag="OO161_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5461543.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSESAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(27149..27466)
                     /gene="hspQ"
                     /locus_tag="OO161_000355"
     CDS             complement(27149..27466)
                     /gene="hspQ"
                     /locus_tag="OO161_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5461544.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(27524..28714)
                     /gene="rlmI"
                     /locus_tag="OO161_000356"
     CDS             complement(27524..28714)
                     /gene="rlmI"
                     /locus_tag="OO161_000356"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5461545.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            28809..29087
                     /gene="yccX"
                     /locus_tag="OO161_000357"
     CDS             28809..29087
                     /gene="yccX"
                     /locus_tag="OO161_000357"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5461546.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(29084..29413)
                     /gene="tusE"
                     /locus_tag="OO161_000358"
     CDS             complement(29084..29413)
                     /gene="tusE"
                     /locus_tag="OO161_000358"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5461547.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(29504..30163)
                     /gene="yccA"
                     /locus_tag="OO161_000359"
     CDS             complement(29504..30163)
                     /gene="yccA"
                     /locus_tag="OO161_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5461548.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQQPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(30370..30457)
                     /locus_tag="OO161_000360"
     tRNA            complement(30370..30457)
                     /locus_tag="OO161_000360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30421..30423),aa:Ser,seq:tga)
     gene            30884..32002
                     /gene="hyaA"
                     /locus_tag="OO161_000361"
     CDS             30884..32002
                     /gene="hyaA"
                     /locus_tag="OO161_000361"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5461549.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            31999..33792
                     /gene="hyaB"
                     /locus_tag="OO161_000362"
     CDS             31999..33792
                     /gene="hyaB"
                     /locus_tag="OO161_000362"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5461550.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAPYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            33811..34518
                     /gene="hyaC"
                     /locus_tag="OO161_000363"
     CDS             33811..34518
                     /gene="hyaC"
                     /locus_tag="OO161_000363"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5461551.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            34515..35102
                     /locus_tag="OO161_000364"
     CDS             34515..35102
                     /locus_tag="OO161_000364"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5461552.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPADISAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            35099..35497
                     /gene="hyaE"
                     /locus_tag="OO161_000365"
     CDS             35099..35497
                     /gene="hyaE"
                     /locus_tag="OO161_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5461553.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            35494..36351
                     /locus_tag="OO161_000366"
     CDS             35494..36351
                     /locus_tag="OO161_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5461554.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQD
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            36485..38029
                     /gene="appC"
                     /locus_tag="OO161_000367"
     CDS             36485..38029
                     /gene="appC"
                     /locus_tag="OO161_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5461555.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHLLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            38041..39177
                     /gene="appB"
                     /locus_tag="OO161_000368"
     CDS             38041..39177
                     /gene="appB"
                     /locus_tag="OO161_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5461556.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            39190..39282
                     /gene="cbdX"
                     /locus_tag="OO161_000369"
     CDS             39190..39282
                     /gene="cbdX"
                     /locus_tag="OO161_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5461557.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            39362..40660
                     /gene="appA"
                     /locus_tag="OO161_000370"
     CDS             39362..40660
                     /gene="appA"
                     /locus_tag="OO161_000370"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5461558.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPSGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(40775..42955)
                     /gene="etk"
                     /locus_tag="OO161_000371"
     CDS             complement(40775..42955)
                     /gene="etk"
                     /locus_tag="OO161_000371"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5461559.1"
                     /translation="MTTKNMNTPPSSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(42975..43421)
                     /gene="etp"
                     /locus_tag="OO161_000372"
                     /gene_synonym="yccY"
     CDS             complement(42975..43421)
                     /gene="etp"
                     /locus_tag="OO161_000372"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5461560.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(43409..44548)
                     /locus_tag="OO161_000373"
     CDS             complement(43409..44548)
                     /locus_tag="OO161_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5461561.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLCQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(44594..46690)
                     /locus_tag="OO161_000374"
     CDS             complement(44594..46690)
                     /locus_tag="OO161_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5461562.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPLDLMTIGPNRNRTVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(46690..47436)
                     /locus_tag="OO161_000375"
     CDS             complement(46690..47436)
                     /locus_tag="OO161_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5461563.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPSALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(47433..48077)
                     /locus_tag="OO161_000376"
     CDS             complement(47433..48077)
                     /locus_tag="OO161_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5461564.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDSSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(48184..48489)
                     /locus_tag="OO161_000377"
     CDS             complement(48184..48489)
                     /locus_tag="OO161_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HCR5461565.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(48931..49143)
                     /gene="cspH"
                     /locus_tag="OO161_000378"
     CDS             complement(48931..49143)
                     /gene="cspH"
                     /locus_tag="OO161_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5461566.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            49429..49641
                     /gene="cspG"
                     /locus_tag="OO161_000379"
     CDS             49429..49641
                     /gene="cspG"
                     /locus_tag="OO161_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5461567.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            49652..49840
                     /locus_tag="OO161_000380"
     CDS             49652..49840
                     /locus_tag="OO161_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5461568.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
BASE COUNT        12303 a        12304 c        13113 g        12135 t
ORIGIN      
        1 aactacgctc tttcaggcag tttagttgcc cattcacagg gcattaccct ggggcaatcg
       61 ctgggggaaa ctaacgtgct gattgcagca ccaggcgcag agaatactcg tgtggcgaac
      121 agcaccgggc tgaaaactga ctggcgcgga tataccgttg tcccctatgc cacttcttat
      181 cgggaaaatc gaatcgcact tgatgcggca tcgttaaaac gtaacgtgga tcttgaaaat
      241 gcagtagtca acgtggttcc caccaaaggg gcgttggttc tggcggagtt caatgcccat
      301 gcgggagcaa gggtattaat gaaaacatca aagcagggta taccgctgcg ttttggcgcg
      361 atagcgacgc tggacggcat acagactaat agcggcatta ttgatgatga tggctcgctc
      421 tatatgtccg gtttgcccgc tcagggagcg ataactgtac gctggggcga agctcccgat
      481 caaatttgtc atatcagtta ctagcttacc gaacaacaaa ttaactctgc gattacgcga
      541 atggatgcca tatgcagata atctttggag aaaaatgcgt gttattacta cgactatttt
      601 ttgccgccgt cttaatgcta tggtgcgctc aaactgctgc ttatagcggg cagtgtcata
      661 ccactcaggg gaatccgtat attggcgtca attttggcgt taaaaccctg gaggaagaag
      721 aaaatacggc tggagtagtt aaagacaaat tttatcagtg gaacgaatcg aatgattatt
      781 atgtttcctg tgattgcgat atagacaatg tcagaagtgg ccgatgggca ttcgccgcgg
      841 attcaccgtt agtctattta ggcgacaact ggtacaaaat taatgactat cttgccgcca
      901 aagttttatt gcaggttaaa ggcagttctc ctactgcggt tcctttcgaa aacgtgggca
      961 caggggcaga tacacgatgg catatttgcg atcccggcgg tcaacgttta ggtggccagg
     1021 gggctagcgg taatagcggt agcttttccc tgaaaatatt gcagccgttc gttggttcgg
     1081 tcgtcattcc tcctatggcg ctggcgcgat tatttgaatg ctacaacata cccgcaggtg
     1141 attcctgcac gactacaggt acaccggttt tagtgtatta cctgtctggt actatcaatt
     1201 cacttggctc atgttccgtc aatgccggag aaacaatcga ggtcgatctg ggcgacgtat
     1261 ttgcggctaa ctttcgtgtt gtagggcata agcctcttgg ggccagaacg gcagaacttg
     1321 caattccagt caggtgtaac acgggaaacg cggggttagt taacgtcaac ctgagtctga
     1381 cggcaactac agaccccagc tatccctagg cgattaagac gtcacgtcct ggcgtgggcg
     1441 tggtggtgac cgatagccag aacaacatta tttcccctgc tggtggaaca ttaccgctct
     1501 ctattcctga tgatgcagac agtatcgcgc gaatgaatgt ctatccagtc agcacgacag
     1561 gtgtaccacc agaaaccggg cgatttgaag ccacggcaac ggtgagaata aattttgatt
     1621 aacgcgtgaa cgtatgaaga aaaaaacgat atttcagtgc gttatcttgt tctttagcat
     1681 tcttaacatc catgtcggga tggctgggcc tgaacaagtt agtatgcata tttatgggaa
     1741 tgtggtcgat cagggctgtg atgtcgccac caaaagtgca ttacaaaata ttcatattgg
     1801 tgattttaat atcagtgatt ttcaggctgc gaataccgta agcactgctg ctgatttgaa
     1861 tatcgatatc accggttgtg ccgctggtat tactggcgcg gacgtccttt ttagcggcga
     1921 ggctgacacc cttgcgccga cactgctcaa actaactgac acaggcggaa gcggtggtat
     1981 ggcaacgggg attgccgtgc aaattcttga tgcgcaaagt cagcaagaaa tcccgctcaa
     2041 tcaggtccag cctcttacgc ccttaaaagc cggggataac acactcaaat atcaacttcg
     2101 ttataagtcc acaaaggcgg gagcaacggg cggtaatgcg acggcggtta tctattttga
     2161 tctggtttac cagtgaagga gtgatgttga aacggataat ctggattctg ttcttactgg
     2221 gattaacggg gggctgtgag ctatttgccc atgatggcac ggtcaacatt agcggatcgt
     2281 ttcgccgtaa tacatgcgtg ctggcacagg atagcaagca aatcaatgtg cagttaggcg
     2341 atgtctcgct gacacgtttt tttcatggga attatggccc ggagaaatct ttcatcatta
     2401 atttgcagga ttgcggaaca gatgtcagca cggttgacgt caccttttca ggaacccccg
     2461 acggagtgca gagcgaaatg ctgtccattg aaagtggtac ggatgcagcc agcgggctgg
     2521 caattgcgat tctggatgat gcgaagatat tgattccgct taaccaggcg agtaaagact
     2581 acagcctaca tagcggtaag gtgccattaa ctttttatgc gcaattgcga cctgtcaata
     2641 gcgatgtgca gtcgggtaaa gtgaatgcca gcgcgacatt tgtacttcat tatgactaat
     2701 acgtggaaca gattggcgct cttgattttc gccgttttat cgctgctggt ggcgggtgaa
     2761 ttgcgggcag gagtggtggt cggcggaacg cgatttatct ttccggcaga cagagaatcg
     2821 atatctattt tactgactaa taccagtcag gaatcctggc tgattaacag taaaatcaac
     2881 cgcccaacgc gttgggccgg gggggaagcg tcgacagtgc cagcaccatt actggccgct
     2941 ccgccactta ttctcctgaa gcccggtact acaggcacgt tgcgcttgct gagaacggaa
     3001 agcgacatct tgcctgtgga tcgcgaaacg ctatttgagt taagcattgc cagcgtgcca
     3061 tccggcaaag ttgaaaatca gagcgtaaaa gtggcgatgc gctcggtatt taaactgttc
     3121 tggcgacccg aaggtttgcc gggcgacccg ctggaagctt atcaacaatt acgctggaca
     3181 cggaattcgc agggtgtaca actcactaac ccaacgcctt actacattaa cctgattcag
     3241 gtgagcgtaa atggtaaagc gttaagtaat gcgggagttg tgccgcctaa aagccagcgt
     3301 cagacaagct ggtgtcaggc aatcgcaccg tgtcatgtcg cctggcgggc gataaatgat
     3361 tacgacgggt tgagtgcaaa gaaggagcaa aatctgccct gaaacaggtt cggaaaacgt
     3421 ttgcgttttt tttgccgcag gtcaattccc ttttggtccg aactcgcaca taatacgccc
     3481 ccggtttgca caccgggaat ccaggagagt tcatgtacta ccccttcgtt cgtaaagccc
     3541 ttttccagct cgatccagag cgcgctcatg agtttacttt tcagcaatta cgccgtatta
     3601 caggaacgcc gtttgaagca ctggtgcggc agaaagtgcc tgcgaaacct gttaactgca
     3661 tgggcctgac gtttaaaaat ccgcttggtc tggcagccgg tcttgataaa gacggggagt
     3721 gcattgacgc gttaggcgcg atgggatttg gatcgatcga gatcggtacc gtcacgccac
     3781 gtccacagcc aggtaatgac aagccgcgtc tctttcgtct ggtagatgcc gaaggtttga
     3841 tcaaccgtat gggctttaat aatcttggcg ttgataacct cgtagagaac gtaaaaaagg
     3901 cccattatga cggcgtcctg ggtattaaca tcggcaaaaa taaagatacg ccagtggagc
     3961 agggcaaaga tgactatctg atttgtatgg aaaaaatcta tgcctatgcg ggatatatcg
     4021 ccatcaatat ttcatcgccg aataccccag gattacgcac gctgcaatat ggtgaagcgc
     4081 tggatgatct cttaaccgcg attaaagata agcaaaatga tttgcaagcg atgcaccata
     4141 aatatgtgcc gatcgcagtg aagatcgcgc cggatctttc tgaagaagaa ttgatccagg
     4201 ttgccgatag tttagttcgc cataatattg atggcgttat tgcaaccaat accacactcg
     4261 atcgttctct tgttcaggga atgaaaaatt gcgatcaaac cggtggctta agtggtcgtc
     4321 cgcttcagtt aaaaagcacc gaaattattc gccgcttgtc actggaatta aacggtcgct
     4381 taccgatcat cggtgttggc ggcatcgact cggttatcgc tgcgcgtgaa aagattgctg
     4441 cgggtgcctc actggtgcaa atttattctg gttttatttt taaaggtccg ccgctgatta
     4501 aagaaatcgt tacccatatc taattatttc ttcgaattcc tctttataag ataatcaggg
     4561 ctttattttc agccctggtt gttttatatt catcgctgtt gcttatttag acattttgta
     4621 cttttattgt tgaggttatt aagcgaagcg acaatggatt gtgttgctgc ggttttatag
     4681 ggtaggggag aggcagatgc gaattaaacc agacgataac tggcgttggt attacgatga
     4741 agagcacgat cgaatgatgc tcgatttagc caatggtatg ctatttcgct cacgttttgc
     4801 gcgcaagatg ttgactccag atgccttttc ttccgcaggc ttttgcgttg acgacgccgc
     4861 gctctatttc tcttttgaag aaaagtgccg cgattttaat ttatctaaag aacaaaaagc
     4921 cgaactggtg ctgaatgcac tggtagcaat tcgctatctc aaaccgcaaa tgccgaaaag
     4981 ctggcatttt gtttcccatg gtgaaatgtg ggtgcccatg ccgggcgatg cggcctgcgt
     5041 ttggttgagc gatactcacg agcaggtcaa tttgctggtc gttgaatctg gcgaaaatgc
     5101 cgcactatgc ctgctggcac aaccctgcgt tgttattgcg ggtcgcgcga tgcagttggg
     5161 cgacgccatt aaaatcatga acgacaggct gaaaccgcag gttaatgttg acagcttcag
     5221 cctcgaacag gcagtctaac gcgccaactt aagtgcagtc ttcggtacac agctacagca
     5281 aagaatggtg ccatcatcgc ccattgctga ttttttcagc ggcgagactt cgccttctaa
     5341 aatctgaaca cggcaacttc cacaaatgcc cgcgcggcaa gaataaggga tacgaattcc
     5401 ctgattttct aattgctcca gcaacacctg ttggttattt ccacgaaatg cctgtccctg
     5461 ccagtcaata tctacatttg cgtccggttg ttgcgtgatg ttggcggtat catcagcggc
     5521 agctgcgccg taaatttttg ccggagccgt tgccagaatt tccacctcat cgccaacgcg
     5581 aatcacgccg ctattacggg caattaaatt ctgaccaaaa tcgacatcgc cgttatcctg
     5641 ggcagtgcgg aaagattgca atgtttttaa tggttcgcct gccggatgtt tttgcccttt
     5701 ttctgggctg acggtggtga aaatacagcg gctacaaggt ttaaccacat caaacaccac
     5761 atcaccaatg cgaatcactt tccagcgatc ttcttcccag gctgacgcgc cggaaaccac
     5821 cagattgggg cggaattgct ccatttttac actggccgga caacgttgtt ggagatcacg
     5881 taacgaggct tcgttagcaa gaaggtaagg atagccatca gcaaatgaca gaggtacagt
     5941 gttgtggcgt ttcacgcgcc gggtcatttg tggccccacc cagcgtaatt gcacttcgcg
     6001 ggagaaaaat ccacttagcc atttgttgat cgcgtctggc gcaattcgcg cggtaaaatg
     6061 tgtgccccaa acttcggttg gtgcgtcttg tgtggcgaaa tcagcaaaac gaacatatgc
     6121 gctactgcca tctggtgcgg tgagatgcaa gccatcatgc acaggtgaag gagtaaaccg
     6181 taccatctgg ggaaactggc gagcggtaat aaacgtaccg tcaggttccg tgatcataaa
     6241 gatgcgatcg aaggccagac cactgacatc agccagagca tgtgtaagac caatgccgcg
     6301 catcgattta acaggatgaa taaaaagccg ggttaatgtc gccacggtgc ggtcctcaaa
     6361 tgaaaataag ccctcaactt tatgacatga cgcgcttatt agctataatg cgcaacaatt
     6421 ttcttagcgc ctgtcccatt aggctatttt atttgtcagt ttggccctgg gttgtgctcg
     6481 aaatcctcac gtacttgtgt acgctccggt ttctccgcgc tgtccatgtc cagactgact
     6541 gcaacaatta cgcctactgc ggtaggctct tagagtaaaa gtgacgatat gaattctctg
     6601 tttgccagta cggcccgtgg gctggaagag ctgttaaaaa ctgaactgga aaacctgggg
     6661 gccgttgaat gccaggtggt tcagggtggg gtccatttca agggcgacac acggcttgtt
     6721 taccagagcc tgatgtggag ccgcctggcc tcgcgtatta tgttgccgct gggcgagtgt
     6781 aaggtttaca gcgatttaga cctctatctc ggtgttcagg cgatcaactg gacagagatg
     6841 tttaatcctg gcgcgacctt cgctgtccac ttcagtggtt tgaatgacac catccgcaac
     6901 agtcagtacg gcgcgatgaa agtgaaagac gcgatcgtcg atgctttcac gcggaaaaat
     6961 ctgccgcgtc caaatgttga tcgcgatgcg ccggatatcc gcgttaacgt ctggctgcat
     7021 aaagaaaccg ccagtatcgc tcttgatctg agtggtgatg gtttacatct gcgtggctat
     7081 cgcgatcgtg ctggtattgc gccgatcaaa gaaaccctgg cagccgctat tgtgatgcga
     7141 tccggctggc agcctggaac accgctgctc gatccgatgt gtggttccgg tacgttgctg
     7201 attgaagcgg cgatgctggc gaccgatcgc gcacccggct tgcaccgtgg gcgttggggc
     7261 tttagcggct gggcgcagca tgatgaagct atctggcagg aagtgaaagc ggaagcgcaa
     7321 actcgcgccc gtaaaggcct ggctgagtat agctctcatt tttacggttc ggacagcgac
     7381 gcacgggtga ttcaacgtgc acgcactaac gcccgtcttg cggggattgg tgaactgatc
     7441 acctttgagg tgaaagatgt cgcgcaactg accaatccgc tgccgaaagg gccgtacggc
     7501 acagtgttga gcaacccgcc atacggggaa cgtctggaca gcgaaccggc gctgattgcg
     7561 ctgcatagcc tgctgggtcg gatcatgaaa aaccagtttg gtggctggaa tctctctttg
     7621 tttagtgcct cgccggatct gctaagctgc ttgcagctgc gtgcagacaa acagtacaag
     7681 gcgaaaaacg gcccgctgga ctgcgtacag aaaaattacc acgttgccga aagcactcca
     7741 gacagcaaac cggcgatggt agcggaagac tacgccaacc gcctgcgtaa gaacctcaaa
     7801 aaattcgaga agtgggctcg ccaggaagga attgaatgtt accgcctgta tgacgccgat
     7861 ctgccggaat ataacgttgc cgttgaccgt tattccgact gggtggtggt gcaggagtat
     7921 gcaccgccaa aaactattga tgctcacaaa gcgcgtcagc gtctgttcga tattatcgct
     7981 gcaaccattt cggtactggg gattgcgcca aacaaactgg tgctgaaaac ccgtgaacgc
     8041 cagaagggca aaaaccagta ccagaaactg ggcgagaagg gcgagtttct tgaagttacc
     8101 gaatataacg ctcacttgtg ggtgaacctg acggattatc tcgatactgg tctgttcctc
     8161 gatcaccgta tcgcccgccg tatgctcggt cagatgagca gaggcaaaga tttcctcaac
     8221 ctgttctctt ataccggtag cgccaccgtg catgcgggat taggcggcgc gcgcagcacc
     8281 accaccgtgg atatgtcgcg tacttatctg gagtgggcag aacgcaacct gcgtctgaat
     8341 ggcctaaccg ggcgtgcgca tcgcctgatt caggccgatt gcctggcgtg gctgcgtgag
     8401 gcaaatgaac agtttgatct gatctttatc gatccgccaa ccttctctaa ctcaaaacga
     8461 atggaagatg cgtttgatgt tcagcgcgat catctggcgc tgatgaaaga tttgaaacgt
     8521 ctgctgcgtg caggtgggac gatcatgttc tcgaacaaca aacgtggctt ccgtatggat
     8581 ctcgacggcc tggcaaaact gggactgaaa gcacaagaaa ttacgcaaaa aacgctctcc
     8641 caggatttcg cccgtaaccg ccagatccac aactgctggc tgattaccgc agcctgaaag
     8701 gaatagtaat gtcattaatc agtatgcatg gcgcatggct gtcgttcagc gacgcgccgc
     8761 ttctcgataa cgcagaactg catatcgaag ataacgaacg tgtttgtctg gtgggccgca
     8821 acggcgcggg caaatcgacg ttaatgaaaa tcctcaaccg tgaacaaggg ctggatgacg
     8881 gtcgcattat ttacgagcaa gatttgattg tagctcgtct gcaacaggat ccgccgcgta
     8941 acgttgaggg tagcgtttat gatttcgttg ccgaaggcat tgaagaacaa gcggaatatc
     9001 tgaaacgcta tcacgatatt tcgcgcctgg tgatgaacga cccgagcgag aaaaatctca
     9061 acgaactggc gaaggttcag gaacagttgg atcaccacaa cctgtggcag ctggaaaacc
     9121 gcatcaacga agtgctggcg caactggggt tagatcctaa tgttgcgctg tcgtcgcttt
     9181 ccggcggctg gttgcgtaaa gcggcattag gacgcgcgct ggtgagtaat ccgcgcgtgc
     9241 tgttgcttga tgaaccgaca aaccacctgg atattgaaac catcgactgg ctggaagggt
     9301 tccttaaaac cttcaatggg acgattattt tcatctccca cgaccgttcg tttatccgca
     9361 atatggcgac gcgcattgtt gatctcgatc gcggcaagct ggtgacctat ccagggaatt
     9421 acgaccagta cctgcttgaa aaagaagaag ccctgcgcgt ggaagaacta caaaatgccg
     9481 agttcgatcg caaactggcg caggaagagg tgtggatccg ccaggggatc aaagcacgcc
     9541 gtacccgtaa tgaaggccgc gtacgcgccc tgaaagcaat gcgtcgcgag cgtggtgaac
     9601 gtcgcgaagt gatgggtacc gcaaagatgc aggtgggaga ggccagccgc tccggtaaga
     9661 tcgttttcga aatggaagac gtttgctacc aggttgacgg taagcaactg gtgaaagatt
     9721 tttcggccca ggttctacgt ggcgacaaaa ttgtcctgat tggtccgaat gggtgcggca
     9781 aaaccacgct gctgaaactg atgctcggtc agcttcaagc ggacagcggg cgtattcacg
     9841 ttggcaccaa actggaagtg gcttatttcg atcagcaccg cgcggaactg gatccctata
     9901 aaacggtgat ggataacatt gccgaaggta agcaagaggt gatggttaac ggcaagccac
     9961 gccacgtatt gggctatttg caggactttc tgttccatcc gaaacgggcg atgacgccgg
    10021 tacgtgcgct ttctggcggt gagcggaacc gcttgctgct ggcgcgtttg ttcctcaaac
    10081 caagcaactt attgattctt gacgaaccga ccaacgatct tgatgtcgaa acgctggaac
    10141 tgctggaaga actgatcgac agctatcagg gcacggtatt gctggttagc cacgatcgtc
    10201 agtttgtcga taacaccgtt acagaatgtt ggatcttcga aggcggcggt aaaattggtc
    10261 gttatgtcgg cggttatcat gatgcccgtg gtcagcaaga gcagtatgtg gcgctcaaac
    10321 agcttgcggt gaaaaaaacc gaagaagccg ccgcggcaaa agcagaaact gtaaaacgca
    10381 gcagtagcaa actaagctat aaattgcagc gcgaactgga gcagctaccg caattgctcg
    10441 aagatctgga ggcgaagctg gaagccctac agacgcaggt ggcggatgct tccttcttca
    10501 gtcagccaca tgagcagaca caaaaagtgc tcgccgatat ggctgctgca gagcaggagc
    10561 tggagcaagc ctttgaacgc tgggagtatc ttgaagcgtt aaaaaatggt ggctgatcgc
    10621 aaaatagtcg atttaaacag agacagctga caacggtaaa attgcctgat gaactgggtt
    10681 tatcaggcat gaaaaccaac actttgttag tcatctgtaa aatagcgcat cattaaggag
    10741 taccaatgtg cgaacatcat catgccgcga agcacatcct gtgctcgcag tgtgacatgc
    10801 tggtggcgtt accgcgcctt gagcatggtc agaaagcggc atgtccccgg tgtggcacaa
    10861 cgttaaccgt ggcgtgggat gcccctcggc agcgtccgac cgcctatgcg ttggctgcac
    10921 tgttcatgct gttgctgtcc aacttgtttc cttttgtgaa tatgaacgtt gcaggagtta
    10981 ccagtgaaat tacattactg gaaattcccg gcgtgctttt ttctgaggac tacgccagcc
    11041 tcggcacctt tttcctgttg tttgtgcaac tggttcccgc gttttgtctg ataaccattc
    11101 tgttactggt gaatcgcgcg gaattaccgg tccgtttaaa agagcaactg gcacgggtgc
    11161 tttttcaact caaaacctgg ggaatggcgg agattttcct cgcgggagtg ctggtcagtt
    11221 tcgttaaact gatggcttac ggcagcattg gggtaggcag cagctttctc ccctggtgtt
    11281 tattttgtgt cctgcaactg cgcgcttttc agtgcgttga tcgtcgctgg ttatgggacg
    11341 acatcgcccc gatgccagaa ctgcgccagc cgctaaaacc aggcgtcacg gggatacgtc
    11401 aggggctgcg ttcgtgctcc tgttgtacgg caatccttcc tgctgatgaa cccgtgtgcc
    11461 cgcgttgtag taccaaaggg tacgttcggc gtagaaacag cctgcagtgg acactcgcgc
    11521 tgcttgtaac gtccatcatg ctgtatcttc cggctaatat tttgcccatc atggtgacgg
    11581 atttattagg ctcgaagatg ccgtcgacga ttctcgctgg ggtcattctg ttatggagcg
    11641 aaggctctta tcccgtcgct gcggtgatct ttctggccag tattatggtg ccaacgttaa
    11701 agatgatcgc catcgcgtgg ctgtgttggg atgccaaagg gcatggcaag cgcgacagtg
    11761 aaagaatgca tttgatttat gaagttgttg agtttgtagg ccgctggtcg atgattgacg
    11821 ttttcgttat cgcggtgctc tcggcgctgg tgcgtatggg aggtttaatg agtatttatc
    11881 cggcaatggg tgcattaatg tttgctttag tcgtcataat gacaatgttt tctgctatga
    11941 cgtttgaccc gcgtttgtcg tgggatcgtc aacctgaatc agagcatgag gagtcctgac
    12001 agtatggaat ctaataatgg ggaagccaaa atccagaaag tgaagaactg gtctcccgtg
    12061 tggatatttc ctatcgtcac ggcgctcatt ggagcctggg ttctatttta tcattacagc
    12121 catcagggac cggaagtgac cctgatcacc gcgaatgcgg aaggaattga aggtggcaaa
    12181 accaccatta aaagccgtag cgttgacgtc ggcgtggttg aaagcgccac actggctgat
    12241 gatttgacgc acgttgaaat caaagcgcgg ctgaattccg gtatggaaaa attgctgcat
    12301 aaagacaccg tcttttgggt ggtgaaaccg cagattggtc gcgaagggat tagcggcctg
    12361 ggaacgctgc tgtctggagt ttatatcgaa ctgcagccag gcgcgaaagg cagcaaaatg
    12421 gataaatacg atttgctgga ctcgccaccg ttggccccgc ctgatgcgaa aggtatccgt
    12481 gtgattctcg atagcaaaaa agccgggcag ctctcgccag gagatccggt gctgttccgt
    12541 ggctatcggg taggttcggt tgaaaccagc accttcgata cgcaaaaacg caatatcagt
    12601 tatcaactgt tcatcaatgc accttatgac cgactggtga ccagcaatgt tcgcttctgg
    12661 aaagatagtg gcattgcggt tgatctgacg tcagcgggga tgcgtgtgga gatgggctca
    12721 ttgacaacgc tgcttagtgg cggtgttagc tttgatgtgc cggaaggtct ggatttaggg
    12781 cagccagtgg caccgaaaac agctttcgtt ttgtatgatg atcagaagag cattcaggat
    12841 tcgttgtaca ccgatcacat tgattatctg atgttcttta aagattcggt acgcggtctg
    12901 caaccgggag ctccggtaga attccggggt attcgcctgg gtaccgtaag caaagtgcca
    12961 ttctttgcgc cgaatatgcg tcagacattt aacgatgatt accgtattcc ggtactgatt
    13021 cgtatcgagc cagagcggct gaaaatgcag cttggcgaaa atacggatgt tgttgagcac
    13081 cttggcgaat tgttgaaacg tggtttacgc ggatcgctga aaaccggaaa cctggtcact
    13141 ggtgcactgt atgttgatct cgatttctat ccaaatacgc ctgcaataac cggtattcgt
    13201 gaatttaatg gttatcagat tatcccgact gttagcggcg gcctggcgca aatccagcaa
    13261 cgactgatgg aagcgttgga taagatcaac aaactgccat tgaatccgat gattgaacag
    13321 gcaaccagta cgctttctga aagtcagcgc acaatgaaaa acctgcaaac gacgctggat
    13381 agcatgaaca agatcctcgc tagccagtcg atgcagcagt tgccgacgga tatgcagtca
    13441 acgttgcgtg aattgaatcg cagcatgcag ggcttccagc ctggctccgc agcctacaac
    13501 aagatggtgg cggatatgca gcgccttgat caggtgttgc gagaactgca accggtgctg
    13561 aaaacgctca atgagaagag taacgcgctg gtatttgaag cgaaggacaa aaaagatcca
    13621 gagccgaaga gggcgaaaca atgaaaaagt ggctagtgac gattgcagca ctgtggctgg
    13681 ccggatgcag ctccggcgaa attaataaaa actattacca gttacctgtg gtgcagagcg
    13741 gtacacaaag taccgccagc cagggcaatc gtctgttatg ggtagagcag gtcactgttc
    13801 ctgactatct ggcggggaat ggtgtggttt atcaaaccag tgatgtgaag tatgtgattg
    13861 ccaacaacaa cttgtgggcc agcccgttgg atcaacagtt gcgcaacatc ctggttgcca
    13921 acttgagtac gcaactgccc ggctgggtgg ttgcctccca gcctctggga agcgtccagg
    13981 acacgctcaa tgttaccgta acggagttta acggtcgcta tgatggcaag gtcattgtca
    14041 gtggtgagtg gctgttgaac caccagggac aactgatcaa acgtccgttc cgtctggaag
    14101 gagtgcaaac tcaggatggt tacgatgaga tggttaaagt gctggccggt gtctggagtc
    14161 aggaagccgc ttctattgca caagagataa agcgtctacc ttaattataa agatttgtaa
    14221 gtataaccgt ctccggtatg ttgcctgagg cggttttttt gtttctaacg tgcggaaaaa
    14281 tttgttcctc ttcacatttt ttgtacaacc gacatgcccg tgtagctcac aaatatgaca
    14341 gtggcgtgaa ttttgcgcat tgacggcagt tatgattcgc ggtattgctt aactgtgatt
    14401 gcacatttag taatcactgt tttcttttcc accagaaacc agtatgaggg aaacgaggca
    14461 tgaagagaca aaaacgagat cgcctggaac gggcacatca acgtggttat caggccggca
    14521 tcgccggacg ctcaaaagaa atgtgtccct atcagacgct gaatcaaagg tcacaatggc
    14581 tgggaggctg gcgagaagcc atggcggaca gggtagtaat ggcctgattc tgtctcttta
    14641 aaaagaaacc tccgcattgc ggaggtttcg ccttttgata ctctgtctga tgataatcag
    14701 aaggcagacg tatcctggaa cagaccgact ttcaggtcgc tggcggtata gatcagacga
    14761 ccatcaacca gcacttcgcc atctgccaga cccataatca gacgacgatt aacaatgcgt
    14821 ttaaagtgaa tacggtaggt cacttttttc gctgtcggca gtacctgacc agtgaatttc
    14881 acttcgccaa cgcccagcgc gcggccttta ccttcgccgc ccagccagcc gaggtagaac
    14941 cctactagct gccacattgc gtccaggccc aggcatcccg gcataaccgg atcgccaatg
    15001 aagtggcatc cgaagaacca cagatccgga ttgatatcca gttctgcttc aacatagcct
    15061 ttgtcgaagt taccacccgt ttcggtcatt ttgaccacac ggtccatcat cagcatgttc
    15121 ggtgctggca attgcgggcc tttagcgcca aacagttcac cgcgaccaga ggcaagaagg
    15181 tcttcttttg tataggattc gcgtttatct accatgttct ctgtaagcct tattttattg
    15241 aagcacgcag gatagctaac acgtgtacgc tgaacaagtc cgatcagttc ggaataaacc
    15301 agttcagcca acgtaatggc catggaaaac ggtgacgtcc ttcctgttgc gatgcttgcg
    15361 cgatacgttc ctggatggtt tgcatcagcg tcgtttggcc ttcgccatcc cacaccagat
    15421 ttaataataa cggcagtgcg tcagtcacat cgtctactgc ccagatggtg aatttgcctt
    15481 cttctaccgc tttcaccagt tcactgtgaa gacttaaatg gcgaacgtta gctgtgggga
    15541 taatgacacc ttgtttcccg gttaactcac gttgctggca aatagcaaag aagccttcga
    15601 ttttctcatt taaaccaccg accggctggg cgcgaccgaa ctgatcgact gaacctgtga
    15661 tagcgatact ctgattcacc ggcacatcgg cgagggcgct tatcagggcg cagagttcag
    15721 ccatcgaggc actatctcca tcaacttcac tgtatgactg ctcaaatgtc agcgatgctg
    15781 agaaggggat ctgttgctca agctgtagtt ccgacatcag gaacgcttgc atgatcatca
    15841 tccctttcgc atggatattg ccgccaagtt ccgctttgcg ttcgatgtcg gtgaattcac
    15901 catcgccaat atgcacaacg cagctaatgc gagaaggttc gccaaaagcg cgtggatgac
    15961 ccggaaattc aatgaccgaa agggcgttaa tttgcccgat gcgttcgcct tcggtttcaa
    16021 tcaggatttg ctcctgaagg atctcatcct gcatacgttc agcgaggaaa ccttcgcgcc
    16081 attcacgctg ctgcagcatt aagtttagct gctcgccgga gaaggtgtcg ccatcacaca
    16141 gggaggcgac ctctttacac tggcggagga tccactgcgg gctaagcgga agtgtttctt
    16201 gttcaccggt gtagcgtgct gcttcgcgga taagtaccgg ccaggcatcc gctcccgggg
    16261 caggtaagtg attatgtctg gcggtaaatg tcacccagcg acaccactgg cttactgact
    16321 ccgcatcgac aatctgcaga gtatcttcaa attcgctata aatagcctgc tctgaaagct
    16381 ctggctccat ctcctggaaa tcagccaatg attcgcgttc gcctaccaga atgactttca
    16441 gcttcaatgg catcgaaggc acagagacgg ggagagggcg cgactcatca aacgcaaccc
    16501 agtcaaaacg ctcgcggtta acgatatttt tcagccgcat ccacagcaga ggttgcgcca
    16561 gcagtgtacg caaagagatg atgagaatac cgccatttgc ctgatgcacc agaccaggct
    16621 gcagggtaat gtcgccatta aactggcgca ggcagccaaa gagttgctcc gcttctaccc
    16681 agtcggcagc gacaacttgc gttaaagtcg caaaattatc atctgcactc actgcgtgac
    16741 gtaagcggat ggagtggccg gaaacctcat agtgatcgcc caccagttgg cctgcatcgc
    16801 tttgtagcgt acgcgcggca tcggcaataa gattcagata ctcagactct tccggggcct
    16861 tagccagcat aaaagaggag gatgctcgcg tatgcagcaa ctgctccagc gcaaattgca
    16921 gccgcggttg agtatcactg aataaaggat cgttttcgtc aatcaaatgt ggctgagcaa
    16981 atatttcctg atagctatcg gtatcaggaa ccaggtcacg ccatgcaagt ttcgtaatgg
    17041 tcaaagttga tgttttttag tctgttgtca aagccgcgat tataccgtaa ccggcactac
    17101 agcacacgta gaaagcaccg acaatacttc tggcatgggc gttaaagctc acaggatgga
    17161 gattcttttc ttcactggcc taaaaagctg atattctgta aagagttaca cggtaacatt
    17221 gagatcgcta tgaaatatca acaacttgaa aatcttgaaa gcggttggaa atggaagtat
    17281 ctggtgaaga agcatcgcga aggggagtta attacccgtt acatagaagc cagtgccgcc
    17341 caggaagccg tcgatgtgtt gctctcgctg gaaaatgaac ccgtgctggt aaatggctgg
    17401 attgacaagc atatgaatcc ggaactggtc aatcgcatga agcaaaccat tcgggccaga
    17461 cggaagcgcc attttaatgc ggaacaccag catacgcgca aaaagtcgat cgatctggaa
    17521 tttattgtct ggcaacgtct ggctggtctt gcgcagcgtc gcggtaaaac gctttctgaa
    17581 acgattgttc agctgattga agatgcggaa aacaaagaga aatacgcgaa taaaatgtct
    17641 tctctgaagc tggatctgca ggcattgctg ggtaaggaat aactgacgaa agtcagttca
    17701 atttactaaa ggcaaaaaaa accccgcagc agcggggttt ttctaccaga cgataactta
    17761 agcctgcggc tgagttacaa cgtctttgat acctttaact tcgatctcta cgcgacgatc
    17821 cggagccagg cagtcgatca gtgcagcacg ctgtttcacg ttgtcacagg tgttgccagt
    17881 aaccgggttg gattcgccca taccacgtgc ggagatcttg tctgccggga tacctttgga
    17941 gatcaggtaa tcaacaacag actgagcacg gcgctcggac agaccctggt tgtaagcgtc
    18001 agaaccgatg cggtcggtgt aacccagaac aactacggaa ccgtctttcg gatccaggtt
    18061 gctcagctgg ctgtacagct gatccagagc agcctgacct tccggtttca gggttgcttt
    18121 gttgaagttg aacagaacgt cagacttcag agtgaagtgc ttggtctgta cttccggtgc
    18181 cggagctgga gccggagcaa ctactggagc tgcttcgccc tgaccgaaac ggtaggaaac
    18241 acccaggctc agcatgccgt tgtccggacg agtgccgatg gtgtgtgcgt caccgatgtt
    18301 gttggtccac tggtattcca gacgggtagc gatttcagga gtgatcgcgt actcaacacc
    18361 gccagcgaag accggagaaa cgccggtgtc gtggttttta ccataaacgt tggatttagt
    18421 gtctgcacgc cataccatac cacccagacg agtgtagatg tccaggccgt cagtgattgg
    18481 gtaacccagt ttagcggtca gttgaacgcc ctgagctttg tatgcaccgt tttcaacgct
    18541 gcctttgtac ggcatacgac ctaaccagtc gtaacccatt tcaaagccaa catacgggtt
    18601 aacctggtaa ccaccaaaag caccagcgcc cagttggttt tcatgggtcg ggccattgtt
    18661 gttgatgaaa ccagtgtcat ggtactggga ccagcccagt ttagcaccag tgtaccaggt
    18721 gttatctttc ggagcggcct gcgctacggt agcgaaacca gccagtgcca ctgcaatcgc
    18781 gatagctgtc tttttcattt tttgcgcctc gttatcatcc aaaatacgcc atgaatatct
    18841 ccaacgagat aacacggtta aatccttcac cgggggatct gctcaatatt aactctaccg
    18901 atatcttcgg cttatgccga gcacccctgg cgatgtaaag tctacaacgt agttgaaaac
    18961 ttacaagtgt gaactccgtc aggcatatga aaaaaaagtc ttgtataagg tatgtttaat
    19021 cttttttgtc agcgacaatt tacagaagaa aatcgcggaa accgcttcag acaagcctcc
    19081 gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg acaaaaaaag ttccaggatt
    19141 aatcctaaat ttacttaatg atacaaatta gagtgaattt ttagcccgga aagttgtctc
    19201 gtggcgtgag aggatgcgct taccggacgc ataataaacc ccatagcgtt accttcattt
    19261 gccgcatcaa caagttcagc atgctcttct tcagtcaaat catctgccaa ccaaccgatc
    19321 accacactgt aattgcccgt gcgtaaagcg cgaaccattg actccacagt gtggcaaggg
    19381 gagagctggc taatctgcat tactttcgtt aagggtagcc cagatgcctg aacccattcc
    19441 cgactcagtt tttgttgcgg tgttaaccag agttgccagc gcgattgctg accgagttgc
    19501 tgtaacaatg gcaacagtag aagttgcgtc atcatgggct gatcttcgcg atagacaact
    19561 tcactgataa gcccggctgt agtgttttcc gtagagacac gcgcaatttt acttgctgcg
    19621 gatgagaacg acgaagaacg atgtgcatag cctgaagtgt acataatcaa tccagcccct
    19681 gtgagttact gtatggatgt acagtacatc cagtgacaac aaagatcaac cctattttcg
    19741 gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac ataaattaat cttgcccctt
    19801 aagaataagt tgcctatttt cgtagttaac ggatccgtta atgtgaatca ttcttttatg
    19861 ttatgatttt aaaaggaatt ttatgaaaag cctctcctat aagcggatct ataaatcaca
    19921 agaatacctg gcaacgttgg gcacaattga ataccgatca ttgtttggca gttacagcct
    19981 gaccgttgac gacacggtgt ttgcgatggt ttctgatggt gagttgtatc ttcgggcttg
    20041 tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc tggctgacat ataaaaagtg
    20101 tggccgatcc gttaccctca attactatcg ggttgatgaa agtctatggc gaaatcaact
    20161 gaagctggtg cgtctgtcga aatattctct cgatgcagcg ctgaaagaga aaagcacgcg
    20221 caatacccgg gaaagactga aagatttgcc caatatgtct tttcatctgg aagcgattct
    20281 cggggaggtg gggattaagg atgtacgggc gttacgtata cttggggcaa aaatgtgttg
    20341 gttgcgactg cggcagcaaa acagtctggt gacagaaaag attctgttta tgcttgaagg
    20401 tgccattatc ggcattcatg aagctgcgct cccggtggca cgccgccagg agcttgcaga
    20461 atgggctgac tcacttacgc cgaaacagga gtttcctgcg gaacttgagt aatctggcgt
    20521 tgcagacgac caatctcagg cagtaatgca atcaataatc caacttgttg tacgaccaga
    20581 ggttctttgc tgtctgcccg tggttcaagt tgctgcatcc gctgtttcag gctagctaat
    20641 gcctcattga cgcgttcttc atcagcaggt tgatgatgta acgcgtcatc aacatagcaa
    20701 actgcgtcat caagaaacgc cagaatttca ggattagtta actgctcccg gtgagcaccg
    20761 agggctgaga tatagctggt aaacgtatgg ttaaggcaca gcaaccgaaa cgcggcttcg
    20821 cgaatttgcg gggtaacgtt cggctcgctg gacatatttg ataccaccga cgccagctca
    20881 gcatcacggt tgtgtgcatc gcggcgggca atacgatacg ccagacggtt atcacgcccc
    20941 tgatggtatt gctccagtat ggcatcgaga taccgacagt tggcctctgt ggcgcgttcg
    21001 agcatgcgcg gcagattgcg aaactgccag tcaggccaga tgtagctcac tgccgcccac
    21061 gcaatggcac aaccaatcag cgtatcgatt acgcgaggta acgctacttc aaaaccttca
    21121 cccagtaagt taaaacacag tagcaccaaa agtgtgatga acatcgttgc atgagcgtat
    21181 tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa ccagcagcac cagctgccct
    21241 tccagtgatg gcacaaacca cagcacagga atgccaatgg cgatacctac cagcgtacca
    21301 ataatcctta acttcaggcg gtggcgcgtg gcgttatagt ttggctggca gacaaacaaa
    21361 cttgtcagca agatccaaaa cccgtgatgc attccggtta tttgaatgat ggcgtagccg
    21421 aagcacaaca ccagcgacat tcttaccgca tgacggaaga gggcggattc cggcgtgaag
    21481 tgacggctaa gacgcagcca gatatcactc aacccgtgcg ggctgtcatc agcgagctca
    21541 ttttcgtcat tattatgggg tagtgcctgg gcctgttctg attcaattgt tgccagttgg
    21601 gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg ttttgagtaa atcggcgggt
    21661 gcgccgttat cgcgcatccg ctccagcgca gcatcaatat gcgtaaaagc gcgctcaaaa
    21721 tgcggatcat gttgataagg ctgacgcaac aaaatacagc gtgacagttg ctggcacgcc
    21781 tggccctgca tcgacatcag ccgctgaaaa cggaacagca cgtcgctgtg gcgaaaatgt
    21841 tcacgcaatg tttgatactg aatatgagaa gagctggcac gctcgtgaat atcctgtgcg
    21901 acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac cacgatcgcc acgtaagcgg
    21961 gtcagcagcg agagtttcgt ctgattcaat gtcgccatca gcagaccgtt ggcgagagcc
    22021 aaatcgtaca gcggtgcctg gctttcatct tcaatatcag gatcaaacat gcgcgacttg
    22081 agctcaagat aacgcgccag ttgttcatag caacgcgcca ggttgtcctg cagcgggcgg
    22141 accgggaaca gcagatgacc aataagtgtc aggacgttgt accagacggc accggccagc
    22201 agatacatcg gctgctgata ccagtgctca tacagtgatg ttcccaacat agtgtaaatg
    22261 gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt gacccagacc gccgagcaaa
    22321 atgaagccgc tggtagagag cgttaagcca atcgcaaata gccagggcca gggaaacagc
    22381 aattctactg aggccgaggc gataaaaaag cagaacagcg taatgatgag gttacgcaaa
    22441 cgccccgcca gtcggtcatc gagatcggtc agcgccgctg ccaccatccc cagcgttagc
    22501 ggaatcgtca gttttacatc acccagccac cacggaaacg ctgtggttcc acaaagcgca
    22561 ataaaaatac gcgcgtaata cagccaggcg ctgttccagg tatagcgttt gagcaaagga
    22621 cttagcataa aggccatatt agtggattat tcaaaacgac gacgtgcatt ggcttcacgc
    22681 gcagcttgcg ctgtttctac cgataccacg cgacgaccaa ccggccatag tgcaatagcg
    22741 gcaattttaa agttcgcaat gccgacagga atgccaatga ttgaaataca ttgtgcgatg
    22801 cccgttgcaa tgtgcatcag gcataaccac cagccaaaga aaatcagcca gaaaatattc
    22861 aataccgtac cgccagtatt cagcagcaca tttttgccag ccgggttcag ttcatcgaca
    22921 tgaatagctt cattgccata aggcaccaga gacagtttag tgatctccca gcaggatcgt
    22981 gtcagcggta aggtaaaaat cagcacaata ctgaccagag tcgccaacag ccagcccaga
    23041 gtggtggcaa atccgccaag cacaaagttc agaatgttca aaacggtacg cataaaacct
    23101 cgctttactg tggttttcag taatgggcgg caattgtaac gtttttttgg gctggagcac
    23161 gttttctctg acggttacac tgataagaaa taatttcgtg tggatctaca gagtcatgga
    23221 actgaaagcg acaacgcttg gaaaacgtct ggcacagcac ccttacgatc gggcggtgat
    23281 cctcaatgcc gggattaaag tctccggcga tcgccacgaa taccttattc ctttcaataa
    23341 attactggcg attcactgta agcgtggtct ggtatggggc gagctggaat ttgtactgcc
    23401 ggacgaaaaa gtggtgcgtc tgcacggcac cgaatggggc gagacgcagc gtttttacca
    23461 tcatcttgat gctcactggc ggcggtggag tggcgagatg agcgaaattg cgtctggtgt
    23521 tttacgccag caactggatt tgattgccac gcgcactgga gaaaataaat ggctgacgcg
    23581 tgagcaaacc tctggcgttc agcaacaaat ccgccaggct ttgtcggcgt tgccgttgcc
    23641 ggttaaccga ctggaagaat tcgataactg ccgtgaggcg tggcgtaaat gtcaggcctg
    23701 gttgaaagat attgaaagcg ctcggttgca gcataaccag gcgtataccg aagccatgct
    23761 taccgagtat gcggattttt tccgccaggt cgagtcttca ccgctgaatc cggcgcaggc
    23821 ccgggcagtc gttaatggcg agcattctct gttagtgctg gcaggtgcag gaagcggaaa
    23881 aacgtcggtg ctggtggccc gtgcaggctg gttgctggca cgtggtgaag cgtcccctga
    23941 gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa gagatggacg agcggattcg
    24001 cgaacggcta cataccgaag acattaccgc acgcacgttt catgcgctgg cgctgcatat
    24061 tattcagcag ggcagcaaaa aagttccgat agtcagcaaa ctggaaaatg ataccgctgc
    24121 ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc agcgaaaaga aagcgcaggc
    24181 gaagggctgg cggcaatggc tgacggaaga aatgcagtgg tcagtgccag aaggtaactt
    24241 ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc ctcgatcgct gggtaagtct
    24301 gatgcggatg cacggtggtg cacaggcaga aatgattgcc agtgcacccg aagagattcg
    24361 cgatctgttc agtaaacgta tcaagttgat ggccccgtta ttaaaagcct ggaaaggtgc
    24421 gctgaaggca gaaaacgctg tcgatttttc gggccttatt catcaggcga ttgtgattct
    24481 ggagaaaggt cgctttatca gcccgtggaa gcatattctg gttgatgaat ttcaggatat
    24541 ctcgccgcag cgggcagcgt tgttagcggc attacgcaag caaaacagtc agacgacgtt
    24601 gttcgctgtt ggtgatgact ggcaggcgat ttaccgattc agcggtgcgc aaatgtcgct
    24661 caccaccgct ttccatgaaa actttggtga aggcgaacgt tgtgatttag acacgactta
    24721 ccgttttaac agtcgtatcg gtaaggtggc aaaccgtttt attcagcaga acccaggcca
    24781 gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa aaagccgtca cgttattgga
    24841 tgagagtcaa cttgacgctt tgctggataa gctctctggt tatgccaaac cggaagagcg
    24901 cattctgatc ctggcgcgtt accatcacat gaggcctgcc agcctggaaa aagcggcaac
    24961 acgctggccg aagttgcaaa tcgactttat gaccattcat gccagcaaag ggcaacaggc
    25021 ggattacgtc atcatcgttg gcttgcagga gggaagtgat ggttttccgg ctgcggcgcg
    25081 ggagtcgatt atggaagagg cgctactgcc accggttgag gatttcccgg acgctgaaga
    25141 acggcggtta atgtacgtgg cgctgacccg ggcacgccat cgggtatggg cactgtttaa
    25201 caaagagaat ccctctccct ttgttgaaat actgaaaaat ctggatgtgc cggtggcgag
    25261 aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat attacttcag acggtccgcg
    25321 agataacgct gataatcggg gatcagaata tcgaccgcgt cgttgaaatg cggcgactgg
    25381 attatgaagt ctgccgttgc cacgttggtg gcgaccggaa tgttccatac cgtcgccaga
    25441 cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat ttagtggatc ccagaagaaa
    25501 atcaatacat caattttccc ttctgagatc aatgcgccaa cctgctggtc accccccatt
    25561 gggccactca acatcgcgtt gacgttcatg ccggtcgcgc gggaaattaa gttaccggta
    25621 gtgcctgttg catacagtac gtgttgttcc agtaacggtt gatgccgttc cacccagctc
    25681 atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa tatgtttccg cgcaggtaaa
    25741 gtgcgagtcg tcagttccat aatgtacatc cgtagtcaac tttcctacag attactgtaa
    25801 gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc acaggtacaa aaatctcagg
    25861 aagatggttg tttttccgcc cactgcagga aagtatttcg cgtttgtggg tcagccagtt
    25921 taaaccaata cttcagccgt tgttctgtga gcacctgaga ctgcggtgga atagcatcca
    25981 gcttggaaac acccgaaagc agcgtactgt catctgccat catcgtggca aattgcggta
    26041 gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc gacctcataa tcaatcgttt
    26101 ttgccgttga ggtaatggcg agaatatcca gctttaccgg aatcggcgtc gcatcgccat
    26161 ccaacaattc aaggcgcggc gcggcatcaa aatggttagc ttcccgctca ttctccaggc
    26221 gaggcaggcg aaaattgacc tggttgatga gctgggtatt aaaactgacc accagcggtg
    26281 gggagatata cagccgttct tcactattgg aaagatgaat cgtcttctca acgcgaaaca
    26341 ctaactgatg cggtccatta tccagttcaa tgctgtccgc accgcgcagc agagagctgg
    26401 aaaccttctt gccgtcgagt accagaagat cgacatcggt tgaaagccgc agcgtggtgg
    26461 caaaaacgga taccggcaaa cataatgcaa tcaaggtggt cacgatgccg gttttcatag
    26521 gtagctcctg tcaaaagacc gcccgaataa taatgtgtca aaatttttct ttaatcatac
    26581 ttactaacat atagacatat ttcccgcttt tgctctcatt cattcgtatt agctgcatgg
    26641 ttggcatgtc ggctttgtcg tacactttgt taaacagtct ggaggaaata atgaaagaaa
    26701 ccgatattgc aggcatttta acgtccactc acaccattgc gctggtgggg gcaagcgaca
    26761 aacccgatcg tcccagctat cgcgtgatga aatatctact cgaccagggg tatcatgtca
    26821 ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg ccagcagggg tatggcacgc
    26881 tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt ccgtaattca gaatcggcgt
    26941 ggggcgtggc acaggaagct attgccattg gcgcgaaaac cctctggatg caactggggg
    27001 tgattaatga gcaagcggca gtactggcac gggatgccgg gttgaatgtg gtgatggatc
    27061 gctgcccggc tattgagatc cctcgcctgg gcctggccaa ataaaaaatc cccggaaggc
    27121 aaaaaccttc cggggattcg ttcagggatc agttacgcag acgcggggcc tggagttgtt
    27181 tgcggatggt ctgcgccagc tcatccatgg atggctgttc aggatgttca tcctgcaatt
    27241 cgctacttag ctgggcttca gccaggtagg tatgaaccgg taggccgtta tcgtcctcca
    27301 tcactacgtg ataccacgga gcggcgcgaa gctcgtcatt taccgccagc tcatcaggcg
    27361 acggttcaga gagcgaataa accgggtcga tatccacgac cactccgaga taacctaaca
    27421 gggaatggcg gacctgctgg ccgataccga atttgctggc aatcatagtc acctcccggg
    27481 aaatctttac ctttgatata agggtaaaat tccacatttc aagttacatg acgcgacagg
    27541 caaacccttt cagatatagc ccttccggat aggtagcgat caccggatga tcggctgcct
    27601 gacggaactg ctctataaat tgtacatcac ggccggcatc aattgcggca tccgcgatga
    27661 ttttctgaaa taaatcgctg gtcatcagac cggaacagga gaaagtcagg agaataccgc
    27721 cttcattcag caactgaatc gccagcatgt tgatatcttt atagccacga cacgcgccca
    27781 tcaactggct tttattctca acaaacttcg gcgggtccat cacgataacg tcaaattttt
    27841 caccgcgatc gcgataagta cgcagcaatt taaagacatc atcacggaca aactcagcct
    27901 tgctcagatc cagtttgttc agctcaacgt tctgccgtgc aatatccagc gcttcctggg
    27961 aggtatcaac gctgacaacc tggctgcaac cgcccatcag tgccgatacg gcgaaaccac
    28021 cggtatagga gaaacagttc agcacacgtt tattttcaac gtagcggcgg gtagccaggc
    28081 ggctgtctcg ctggtccagg tagtagcccg ttttgtgtcc gtgctgaata tccaccagca
    28141 gcttcatgcc gtgttcttca atcggcagca gggcaggcgg caactcgccg gtgacgaggc
    28201 ccagggtcag ctccatccct tcttttttac gtaccgcaac atcgctgcga tcgtaaatcg
    28261 cacattccgg gtacagcgtt tgcagggcac taactaatgc cgcgcgctga tattctgccc
    28321 cagcactcag cagttgcagc accagaaaat taccgaaacg atcgatagta atacccggca
    28381 ggccatcaga ttctccggcg attaaacgat agctgtcgag gccatctttt tgcgccagcc
    28441 agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa aaaagcaatg tcgatagact
    28501 cagacgggtc aaacgtccag acgcgcgccc ggatttgcga agctggcgaa taagcgccgc
    28561 gtgctaacca ttttccctga tgatcaacaa tatcgatggt ttcaccgagg ctggctttac
    28621 cttccatgcg ggcaacggcc ccggaaaaga cccacggatg gcgacgaagt aatgattttt
    28681 cgcgcccttt ggctaacact aaacgtacac tcataattta cttttctgtc gatgcaaaga
    28741 aatgggcgtc attgtccgga gttttccatg gatttgcaac gcgtccatta aggataagga
    28801 gagcagcaat gtcgaaagtc tgcataattg cctgggttta cgggcgagtt cagggcgtag
    28861 gatttcgcta caccacacag tacgaagcga aaagactggg tttaaccggg tacgccaaaa
    28921 atcttgacga tggcagcgtg gaagtggttg cctgcggtga agaagggcag gtggaaaaat
    28981 taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg tgtagaacgg gtgctcagcg
    29041 agccgcatca tccctcgggg gaattaaccg attttcgtat ccgttaaata cattttaccg
    29101 gttttggcag gccagcaatt ttggtggctt gcttcgccgg acctttcgga aacagtcggt
    29161 ataaatagcg gctgttacct ttttcttcgc caaatttatt cgccatcgct tttaccagca
    29221 tacgaatcgc cggagaagta ttgaattcca gatagaaatc acgcacaaaa cgcaccactt
    29281 cccagtgttc cggtgacagc gaaatccctt cgttctctgc aatcaccact gccagcggct
    29341 cactccactg gctgctttct ttgagatagc cttcggtatc cgtttctatc tctttacctt
    29401 cgaagatcag cataattatt actaccttaa tcagactggc ggcagtgtaa caaaaaacaa
    29461 agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg agattaatcg cggctggcga
    29521 agcccagaat gctcagcagg ctgacgaaga tgttgtacag cgaaacatac aggctaaccg
    29581 tggcacgaat atagttcgtt tcaccgccat gaacgatgtt gctggtttca aacaaaatag
    29641 cgccagagga gatcaaaatg aagaccgcgc tgatcgccag atgcagagca ggctgctgca
    29701 ggaagatatt agcaaccata ccaatcagca ccaccacaat acccgccatc agcataccgc
    29761 cgaggaacga catatctttg cgggtggtca gcacatatgc agagcagcag aagaacacta
    29821 acgccgttcc gcccagtgcc atagcgatta cgtcacccat tccggcagac agataggtgt
    29881 tcagaatagg tccgaggata taacccagaa aaccggtaaa ggcgaatgcg gagataatcc
    29941 cggtcggctt attcgccgtt ttataggtca ggaacatcaa accatacata cccaccagcg
    30001 tcagaatcag acccggagat ggcagcatca gcaccgtact ggcagttgcg gtaatcgccg
    30061 aaaaggccag cgtcaggctc agcagaaaat aggtattacg cagcacctta tgggtgctaa
    30121 gcagtgatgt acggtcatgt gaagaactaa caatacgatc cattgagtca ctctctatga
    30181 cagatgtaat taattaagca gcataatgat aatgcgtaag ggcacccaga agttttaccc
    30241 atctttacgc atttgatctg gaacaggttt aacagcggat tatcaggtca ttaagcaaat
    30301 ataacgccct gagaatttcg acaggcaaaa gaaaaagggg ttagcattta gctaacccct
    30361 tatcttattt ggcggaagcg cagagattcg aactctggaa ccctttcggg tcgccggttt
    30421 tcaagaccgg tgccttcaac cgctcggcca cacttccgga atgacgcgca ctataaacat
    30481 cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct gaaaaacagc caaaagtgca
    30541 ttgatagcgt gaaataacag cagattgatc atttcatcac catgaattcc ttctctttta
    30601 ctcgtttagc aaccggctaa acatccccac cgcccggcaa aaagaaaaat aggtccattt
    30661 ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt ttcactacgc
    30721 tttattaaca atactttctg gcgacgtgcg ccagtgcaga aggatgagct ttcgttttca
    30781 gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc gaccataagc
    30841 gcccggtgtc ctgccggtgt cgcaaggaga agagacgtgc gatatgaata acgaggaaac
    30901 attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca aatattgtag
    30961 tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg cctgggcgct
    31021 ggagaacaaa ccccgcattc cggtggtatg gatccacggt ctggaatgca cctgctgtac
    31081 cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt ccctgatttc
    31141 cctcgattat gacgatactt tgatggctgc cgccggaacc caggcggaag aagtcttcga
    31201 agacatcatc acgcaataca atggcaaata tatcctcgca gtagaaggta atccgccgct
    31261 gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga aactcaaacg
    31321 tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct ggggctgcgt
    31381 gcaggccgcg cggcccaatc cgacgcaggc aacgcctatc gacaaagtca tcaccgacaa
    31441 acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg ccatcattac
    31501 ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggccgtc cgctgatgtt
    31561 ctatggtcag cgaatccacg ataaatgcta tcgccgcgcc cacttcgacg ccggagagtt
    31621 cgtccagagt tgggatgatg acgctgcccg caaaggttac tgcctgtaca aaatgggctg
    31681 taaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg gcgtttcttt
    31741 cccaatccag tctggtcacg gctgcctagg ctgtgcggaa aatggtttct gggatcgcgg
    31801 ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca ccgccgatac
    31861 cgtcggttta accgcgcttg gcgtggtggc agcggctgtt ggtgtgcacg cagtcgccag
    31921 cgccgttgac cagcgcagac gtcataacca gcaacctaca gaaaccgaac atcagccagg
    31981 caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac catcaataat
    32041 gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat gcgctgcgaa
    32101 gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac catgtttcgc
    32161 gggctggaga tcatcctaca agggcgcgac ccgcgcgatg cgtgggcgtt cgttgaacgt
    32221 atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat cgaagatgct
    32281 atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct ggcaacgctc
    32341 tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg gatcgatgtg
    32401 ttagatgcgc tgaaagccga cccgcggaaa acctccgaac tggcgcaaag tctctcctct
    32461 tggccgaaat catcccctgg ctatttcttc gacgtacaaa accgcctgaa aaaatttgtt
    32521 gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca gtacaaactg
    32581 ccgccagaag ctaacctgat gggctttgcc ccctatctcg aagctctcga tttccagcgt
    32641 gaaattgtca aaatccacgc ggtctttggc ggtaaaaacc cgcatccaaa ctggattgtc
    32701 ggcgggatgc cttgcgccat caacattgac gaaagcggcg cggtcggggc agtcaatatg
    32761 gaacgcctga acctggtgca gtcaattatc acccgcacgg cggacttcat taacaacgtg
    32821 atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga aatcggcact
    32881 ggtctttctg ataaatgcgt tctcagctac ggcgcattcc cggatattgc caacgacttt
    32941 ggcgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt caacaatgtg
    33001 ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgacca cgcctggtat
    33061 cgatatccca acgatcaggt cgggcgtcat ccgttcgatg gcatcaccga cccgtggtac
    33121 aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga acaggaacgc
    33181 tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg gccgctggcg
    33241 cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt cgatcgcatg
    33301 atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttaggccg cattttgtgc
    33361 cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga caagctgatg
    33421 accaacctga aaaacggcaa tctcgccact gcttccacgg aaaaatggga acctgcaacc
    33481 tggccgacag agtgccgtgg tgtcggtttt accgaagcgc cgcgcggggc gttaggccac
    33541 tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc gaccacctgg
    33601 aacgccagcc cacgcgatcc caaagggcag attggagctt atgaagcggc gctgatgaac
    33661 accaaaatgg cgatccccga gcaaccgctg gagatcctgc gtactctgca cagctttgac
    33721 ccgtgcctcg cctgttcaac acacgtgctg ggcgacgacg gtagcgagct gatctccgtg
    33781 caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa cgttgtcagc
    33841 cactatgtct ttgaagcgcc agtccgcatc tggcactggt tgacggtgtt atgcatggcg
    33901 gtgttgatgg tcaccggata ctttatcggc aagccgctac cttccgtcag cggcgaggcg
    33961 acgtatctgt tctatatggg ctacatcagg ttaattcact tcagcgccgg gatggttttt
    34021 accgtggttt tgctgatgcg gatctactgg gcttttgttg gcaatcgata ctcccgcgag
    34081 ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta tgaaatccgc
    34141 tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataatcc catcgcccag
    34201 gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcactgg ttttgcgctg
    34261 tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt ggaatttttc
    34321 tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg gatgtggctg
    34381 attggcgcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat catgtccgac
    34441 gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg caaaataagt
    34501 aacaaggagt gttcatgagc gagcaacgcg tggtggtcat ggggctgggc aacctgctgt
    34561 gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat taccactggc
    34621 ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg gggtatgtcg
    34681 aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa cctggaacgc
    34741 tgcgaaccta tgccggagaa cgcattccgg ctgatatcag cgcgaagaaa atgagcctgc
    34801 atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat ctgccagcac
    34861 atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc agcctgagcg
    34921 aactggcacg ggagcaactg ctcgctgcgg aacaatcggc gctggcgcag ttggctgcgt
    34981 ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac tgtctgtcga
    35041 tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag gagcagggat
    35101 gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg gctggacgcc
    35161 ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg tggtgttatt
    35221 aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga ttggcgaatt
    35281 actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg agcagagcga
    35341 agccatcggc gatcgctttg gcgtctttcg ctttcctacc actttagtgt ttaccggcgg
    35401 aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa acctgatgcg
    35461 cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt ccatctgctg
    35521 gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc gatcacctgt
    35581 caggtgaatg atgaaccgag tatggcggcc ctggagcaat gtgctcacag cccgcaggtg
    35641 attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc gttgggcgag
    35701 gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat caatacgctt
    35761 ctcggggaag gggaagtgtc agtgcgcatt cagcaggatg acgacagtaa aagtgaaata
    35821 caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga aaagttgctg
    35881 gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc aacgcaaaat
    35941 ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa tggcctaccg
    36001 ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca cagcattaat
    36061 ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcacgtct ctgtgggccg
    36121 ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc cacggggtta
    36181 cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact ggaaagttat
    36241 gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt cgactctgcg
    36301 cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta aaaagacggt
    36361 aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt cgtagagttt
    36421 caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc ctacaggagt
    36481 gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg cgctgtatca
    36541 ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca tggaaaccat
    36601 ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg gtaagctctt
    36661 cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc agtttggtac
    36721 taactgggca ttctattcca actatgtggg cgatattttt ggcgcaccgc tggcgatgga
    36781 agcattaatg gccttcttcc tcgaatccac ctttgtcggg cttttcttct tcggctggca
    36841 gcggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg gttcaaatct
    36901 ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg gtgcgcattt
    36961 tgatatcgac accctacgta tggagatgac cagcttcagc gagctggtct ttaacccggt
    37021 cagccaggtg aaatttgtgc acaccgtaat ggcgggctac gtgaccgggg ccatgtttat
    37081 tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgttg cattacgctc
    37141 gtttgccatc ggttccgtct tcggtactct ggcgattatc ggcacgctac aactcggaga
    37201 cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga tggaagggga
    37261 gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg aacaggatca
    37321 agagcgtaac gcctttgcca tcaaaattcc cgcgctgtta gggatcctcg ccactcactt
    37381 attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc cacgcttgca
    37441 acgcggacgt atggcttggc tgttaatgca ggaaatatcg caaggcaatc gtgagccgca
    37501 tgtgttgcag gcattccggg aactggaagg tgacctgggc tacggcatgt tgctctcccg
    37561 ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga tgcgtggcgc
    37621 gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct gtggttccct
    37681 gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa tcgaccagca
    37741 tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg cgattgaagc
    37801 cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca tcttaccgac
    37861 atactccgcg cattccgctt taaccacagg acaactggct ttctcactga tcatgatcgt
    37921 agggctttac accctgttct taatcgccga agtctacctg atgcagaaat atgcccgtct
    37981 ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa ggagaaaatc
    38041 atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt gatcctggtg
    38101 gtctttatga tctccgacgg atttgacatg gggatcggct gtctgctgcc gctggtggcg
    38161 cgtaatgatg atgaacgccg gatagtgata aacagcgttg gtgcacactg ggaaggcaac
    38221 caggtctggt tgatcctcgc tggtggggca ttatttgccg cctggcccag agtgtatgca
    38281 gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcact gttcttccgc
    38341 ccgctggcct ttgattatcg cggaaaaatc gccgatgcac gctggcgtaa aatgtgggac
    38401 gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc cttcggcaac
    38461 ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta tctcggcagc
    38521 ttctggcaac tgctgacgcc attcccttta ttgtgcggat tgctcagcct tgggatggtg
    38581 attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca tctgcgttca
    38641 cagctggcca ccaaacgcgc tgcactgttg gtgatgctgt gctttttgct ggcgggttac
    38701 tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa cggtccttcc
    38761 aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa tttcgtcgaa
    38821 tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct gacggtgatg
    38881 gcgatttatc gtggtcgccc aggttgggga tttttaatgg cgtcattgat gcaatttggc
    38941 gtgattttca cggcaggcat cacgctgttc cccttcgtca tgccgtcaag cgtgagtccg
    39001 atctccagcc tgacgttgtg ggacagcact tccagccagc tgacgctgag cattatgttg
    39061 gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag ctactacaaa
    39121 atgtgggggc gcatgacaac agaaactctc cgccgtaacg aaaacgagtt gtactaagga
    39181 gcaggaacaa tgtggtattt actttggttc gtcggcattt tgttgatgtg ttcgctctcc
    39241 acccttgtgt tggtatggct ggacccgcgt ctgaaaagtt aacgaacgta ggcctgatgc
    39301 ggcgcattag catcgcatca ggcaatcaat aatgtcagat atgaaaagcg gaaacatatc
    39361 gatgaaagcg atcttaatcc catttttatc tcttctgatt ccgttaacct cgcaatctgc
    39421 attcgctcag agtgagccgg agctgaagct ggaaagtgtg gtgattgtca gtcgtcatgg
    39481 tgtgcgtgct ccaaccaagg ccacgcaact gatgcaggat gtcaccccag acgcatggcc
    39541 aacctggccg gtaaaactgg gttggctgac accgcgcggt ggtgagctaa tcgcctattt
    39601 cggacattac caacgccagc gtctggtggc cgacggattg ctggcaaaaa agggctgtcc
    39661 gcagcctggt caggtcgcga ttattgctga tgtcgacgag cgtacccgta aaacaggcga
    39721 agccttcgcc gctgggctgg cacctgactg tgcaataacc gtacataccc aggcagatac
    39781 gtccagtccc gatccgttat ttaatcctct aaaaactggc gtttgccaac tggataatgc
    39841 gaacgtgact gacgcgatcc tcagcagggc aggagggtca attgctgact ttaccgggca
    39901 tcggcaaacg gcgtttcgcg aactggaacg ggtgcttaat tttccgcaat caaacttgtg
    39961 ccttaaacgt gagaaacagg acgaaagctg ttcattaacg caggcattac catcggaact
    40021 caaggtgagc gccgacaatg tatcattaac cggtgcggta agcctcgcat caatgctgac
    40081 ggagatattt ctcctgcaac aagcacaggg aatgccagag ccggggtggg gaaggatcac
    40141 cgattcacac cagtggaaca ccttgctaag tttgcataac gcgcaatttt atttgctaca
    40201 acgcacgcca gaggttgccc gcagccgcgc caccccgtta ttggatttga tcatggcagc
    40261 gttgacgccc catccaccgc aaaaacaggc gtatggtgtg acattaccca cttcagtact
    40321 gtttattgcc ggacacgata ctaatctggc aaatctcggc ggcgcactgg agctcaactg
    40381 gacgcttccc ggtcagccgg ataacacgcc gccaagtggt gaactggtgt ttgaacgctg
    40441 gcgtcggcta agcgataaca gccagtggat tcaggtttcg ctggtcttcc agactttaca
    40501 gcagatgcgt gataaaacac cgctgtcatt aaatacgccg cccggagagg tgaaactgac
    40561 cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt tcgttggcag gttttacgca
    40621 aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa agagcattca
    40681 gttacctgaa tgctctgagg ctgatgacaa acgaagaact gtctaatgcg tagaccggaa
    40741 aaggcgttca cgccgcatcc ggccattttc agttttactc tttctcggag taactataac
    40801 cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca ccattgagga
    40861 tagcgccttt aatattgacg cctgcctgtt ccagacgctg cattgacaaa ctcacctctt
    40921 tggcggtgtt caagccaaaa cgcgcaacca gcaggctggt gccaacagaa cgccccacga
    40981 ccgcggcatc actcaccgcc agcatcggcg gcgtatcgac aatcaccaga tcgtaatggt
    41041 cgttcgccca ttccagtaat tgacgcatcc gatcgcgcat cagcagttca gacgggttag
    41101 gtggcacctg accgcgagta atcacatcaa agcctccttt gccaaaatgc tggatcactt
    41161 tgttgagctc atctttacct gccagatatt ccgacaagcc atgttcatta ctcacggtaa
    41221 acaggttatg cgaataacca cggcgtaagt cggcatcaat aaataacact ttttgatcgg
    41281 actgggcgat caccgctgcc agagttgaac tgacaaacgt tttaccactg tctggcgtcg
    41341 caccggtgat catcagaatg ttattctccg tctccatcat agcgaagtgc agactggttc
    41401 gtagcgcacg tacggcttcc acagcagaat ccgccgggtt atccaccgcc aggaagggga
    41461 tatttttagt acgatggcgc tgctgattag aaaataaatt tttcttacgc agacgggtac
    41521 gtttatccag ccactcggac attgggatag tggcataaac gctgatgccg tgctcttcca
    41581 gttgttccgg ggcttctaca ccacgacgca acatcgcacg cgccagcacg gcacccaccg
    41641 aaataaacag gccaagaata aaaccaagca ccacgttcaa cgcttttttc ggtttcactg
    41701 gctgcggctg agtgactgcc gggtcgataa tccgcacgtt accaatggca ctggatttcg
    41761 aaatactcaa ctcctgctgg cggttaagta attgcagata taccgcacgg cccgcttcta
    41821 cgtcacggct taaacgtaac acttcctgtt gagtggaagg cattgccgat acccgcttat
    41881 tcaggcgttt gcgttcttgc tccagcgtct ggcgtttttc cagcagcgca cgataagttg
    41941 ggtgatcttt cttatacagc tgggagatct ctgcctcgcg gaaagtcagc tcattgagtt
    42001 gattatcaac gttcacaatc tgctcaagaa cggctttggc ttctaggtta aggtcaaccg
    42061 aatcgcgctg ctggcgataa acgttgagtt tttcttccgc ctggtccagc tcgctgcgca
    42121 cttcaggcaa ctggcgctgt aagaattcaa ggctttgtga atcctgcgcc gcctggcgag
    42181 cgatattctg ttgcaaatag ttgttagcga tgctgttcag aatacgagta atcaactggg
    42241 gatcatcacc agtcatggta agttccagca tcccgctttc tttactgcgt tcgctaacgg
    42301 taaaggtttc ctgcaatgcg ttaatcgctt ccagttcggt acgctggctc aggacaaact
    42361 gtgttcctgg tttggcctta atgtccgcga tagtcagcgc aacgccatct ttttccagac
    42421 gctggccgac cataccattg acggtgaact cttcaccttc cagtgtatag tggccgtttt
    42481 ccccaaccgt gagtgtcagt tgctgatcct gaccattcag ttgtggaata tgcatccagc
    42541 tgatcgccag ctcacctggt ttttctttgg ttaatctcgc ccagccgcga cccacaatcg
    42601 gaaaatactt ctgctcaact atgtcgcgca gattcagttc agcaatggtt ttaccgagaa
    42661 tcatgcgcga ttgcagcagt tggatctccg gtgcagactc gggcgatgag ttagggatca
    42721 tatcactcag gccgctgaga atggcgttgc cctgtttttg ctcaacctgg accagagtat
    42781 ctgcctgata aattggtgtg cttaacagcg agtaagcgac agcgatcagc gtgaataacg
    42841 cggtcacgct gataataaac ttacggtgat cccataactc gccgaccaga cgaagcagat
    42901 cgatctcatt ttcctgagtg ctgcttggtg gcgtattcat atttttagtt gtcatcgtat
    42961 tttataaact caaattaccg gctgaggcgc ttcgcccatt cctgactggc gcgctccaac
    43021 ataccgtaga catgttcaaa tgcgtcctga cttttacgat agggatccgg gatctctttc
    43081 tgttccagcc attgcccaaa cagcattgtc ttcccgcgga cctcgggagc gattgcggta
    43141 acctgagcaa tatgctctga ctccattgcc agaatcagat cgtaatttcg tgccatctca
    43201 gcagtgagct tacgtccggc atgtccttca agagatacgc catgattagc ggcaacatct
    43261 gctgctgtcg catctgcagg gtgttttacc agaccatgaa cacctgccga tttgactttt
    43321 acgcctggca gacgtttacg cagcaagcgc tcgccaatgg gcgaacggca aatgttgccg
    43381 gtacaaacca ccaggattga gttaaatttt agttgggcca tgtttgaatc caccgtgaag
    43441 tctctgtcag gtcatgcacc ccggaaatcg tcggtaccag cagagaaatc acacggttcc
    43501 aacgagccag cggcgcggta gtgacataaa caatgtcgta aggttcaagc tggaattccg
    43561 ttccgagtat cattgccgat gcgtccttcg cattcagctg ataaatattg gcgattttgc
    43621 cattctgctt gttttgcgtt gcacgaataa caaaaatccc cgtcgcatca gcgacatcct
    43681 gattcatacc ctcagcgtta cccagcgctt ccgccagggt cataccgctg cggtccattt
    43741 tcagcgtgct ctgtttaccc acttcaccca tcacgaagac tttcagatca tcgttgcgcg
    43801 gaataaataa aatatcgccc gggtgcagca gtttgttctg gcacaagtcg ccacgctgca
    43861 tcagcgcata gaggttcact ttggtcttta cgccattctg ggtgagcacc acgttgcgcc
    43921 agtctgcatc tgcggtcaga ccgcccgcgg cgttaatcgc atccatgatg gtcagaggaa
    43981 tattggtaat cggctgctga cccgatttgc tgacttcgcc ggtgacgtac gctttttgcg
    44041 atcggaacgc cgcgacgctg acatcgactt gcgggctttc aatgactgaa tcgagacgcg
    44101 ctgtaatctc gttgcgtacc tgggtaagtg ttttcccggc cactttcagg cgaccaatat
    44161 aggggtagaa aatcgcacca tcggcgttaa cccagttgcc ggtatcactg gcgctacggt
    44221 attgccctgc gggggtggtt agttccgggt gatcccagac cgtaaccatc aacacatcac
    44281 caatcccgat acgatattca tagtttgcga tttgctgctc cagctccggg ttagcctgcg
    44341 acataatcgg tttggcgcgc aactgatcaa tcaatcctgg cgtgacggga tagatattga
    44401 ccattttgtc caggtcatac tgattgtcag gtagttcaat gacatccttg ttactggtcg
    44461 ataaattctg accaggaaca agggtacatg ctgtcaaaga cagaacagcc aatgtcagta
    44521 ccgaaaactt aaaaatgttc tttttcattg gtttatattt atcagtcaat gtaatgttat
    44581 gaattacgtc aggttattgt ccaaccggta cttctcgctc tgccgtcatt ggatagagct
    44641 ggtatttgcg tgacagcatt tgtccaccat cacgcgtcaa tggtgtccac gaaaccaccg
    44701 tgcggttgcg gttaggtcca atggtcatca agtcgagtgg aatcgagata taaaaacctt
    44761 tactaaagcc gccttcgccg tagtcatctt tagatacgtt actgattgcc gcccataccc
    44821 ctaccgccac gccgctgtca aagcgtttgg cgacgtcgat cgttgcgcct ttatcttttg
    44881 ccagatattg cccaacgcta agtttcatca gtacgccatt gagcgtcggc gggttccagt
    44941 aagccgtcac gaaaccagtt ggcgtggaat aatcggtgaa gcgcatcatg ttgtcccagt
    45001 cacgttgctt cacgtagtta acgtccacac ccagcgccca gctggcatct agcgggcgat
    45061 aaagcagctc ggaaccgaca ccggcgtaca tcgtttccag ataaccgcca tacacctggc
    45121 catagaaacc attgcctaag tcggcaaagt agttcgcctg caagttgttg agataaacgt
    45181 cattgcgaac gtaatcacgg atatgcgtgc gcacgcgggg cagggtagag tccgcgggca
    45241 acagcgaaga cttaaacttg tcgtagttgt tgtaaatatt ggtgaaaata ccgccatcaa
    45301 gcagcaggtg gtcggtaaac cagtagcggg cactggacat cagccccagc tggaacatat
    45361 agaaatcttc cgggccgccc agcgactgtg aaagtgttgg gttgaaagag tagctaaagc
    45421 gatcttcacg aatacggtag ccccgaccaa aggcagaaag atcttctgct tcaacacgtt
    45481 gttgttgaag ttgctctgat tgaccaggcg ctgtaccagc cagctgtttg cgcaggctgg
    45541 ctacgtcggt ttcggtagtc accatcgcca tatgctcgcg cttttgcgtc acgctaatct
    45601 tctcaacgcc ttgcggcagg ttattcacca gaatccggtt ggcacgatcg accgcttcgc
    45661 gagaatcacg gtatttgtat tgttgaccag acatatacag cgtcttatcg cgcagctgaa
    45721 tttccggtgc ttcaaaacct gcgttgtact tcagcgcggt aagttggttc gctactgtgg
    45781 tgtactgcaa tccttcagat tcaggcgcag gttgatatgc cggttttggc gtatcgcgca
    45841 gggcagggcg cagatcgttg aaattggtcc gtaacgtgaa gccaaacatc aacgtgttac
    45901 cgcgttcata actcaggttg agatctgccc agctggcagc gcgataaact gcgccgacgt
    45961 tgaaatggct tgcctgaggc agtttgccag cgaaatcatt ctggtaattg ttgccatcgt
    46021 attcgagttt cagacgcagg ggattccacg gcgtttgata ctcaatgccg ccaaagatgg
    46081 aagccgggcc acgaaagata tcgctaaagc tgatatcgcc cgcatcgtga gactctgcgc
    46141 gatgacaata tttatcgctc acccggcaaa acgggttggt aatattgccc gcattaccgg
    46201 cgtagcccca tgccatcccg agggtgaaat caaatggccc cgcttgcttg ctggccacca
    46261 gatactcacc gtcaaacaga cccgtaccag caatatcacg tttaccaaac gccacttgcg
    46321 gtagccagta accttcttcc cacaggcgaa gtttaaaatc gaatgatttg tctttgtatg
    46381 actgatcgcc gctgaaatct tcccactggc tatatttgcg tgtgcgcaca tccgtataac
    46441 gaatggtgcc ttccagccat gggaacagcg ccacggaggt ggagtagaac cggtattgat
    46501 cgttatcccg gtagttgacg ctgaattcac cttccggtgc gatgcgcgca tttggcatct
    46561 gcaacaatcc tgtgccgcca aagtctgatt gcgacgaacc tagcggatcc gggtaggtta
    46621 atacttcagc atgacaggca ctggagacgg caatggccag gcagcttaaa agataagaat
    46681 tcttcttcat cagtcaggaa cacgttgcgt taaaacagac acaatctggt catttaaatc
    46741 agcatatttt tcgggcagta catgcgcaga aaagcccaac caaagctggc tgccaggcgg
    46801 tggttccaca tgtcttttat tccacagggc aacaggggcg acgacagtct caccctcagg
    46861 cgtaatcacc ataacgttat tcttgtccgc gcctgcaagg cgggggtgat cttgcagata
    46921 atcggtaacg ctacggcctg cctgccaggg caattgcccg gccccagaaa ccgcacccag
    46981 cagagtaata gtcaccggac gctgtacggt atagagcgta tagtccccga ccaacggggg
    47041 attgctgttc tcatccactc gaacaaaatc agggtccagt ttgaccggta agcgaccagt
    47101 gatattgaga ttgagtaact gctggcgcac ggatttaatc gttgccgcca catcgtcgtc
    47161 agcttctgct tcccaagaag caagctgtgc catgacatgt tggtagtctt taagcgcttt
    47221 agctttcgcc gcagagtcgg tcagcaaggc actaggccac cagaggcgat cgcgcagttg
    47281 cggttgtgtc accaactgga caacattttc catggggcca acggaaagtg tctgttgttc
    47341 gccaggcaga taaatagtca ccgttccttg cgcaaaggca tggggtgtca ttacgtaaag
    47401 tacgctggca atgaaatacg actgtaattt attcatggtg cgggtttaag aaacgtcatt
    47461 tctacgggaa taacccctgc gcctaacatt tgccgacttt gacgaacctg gccagtagcc
    47521 gaatcgaccc agaatgtgtt ttgccactgt ttttctggcc gcgtggaagt cacctcttcc
    47581 tgccagacat tacacagcac attgcgaccc gcaatattca gcgtctctaa tccagcaaag
    47641 gaaaaagtag aactcagggt cgcagagcga aaatggttgt cttcactcca gagaatatcg
    47701 cgcgtccagc ggctgctatc ttttattgcc agcgcgttac gcagagggtc ctgcccggaa
    47761 tttgtcactt ccagcagatt attattaagt ttgacggttt ttaaaagacg tccattgtgg
    47821 gtaaccagca tggcgttatc ctgggataac catttgcttt gttcttgttc tatatatccc
    47881 agtaccacaa agattcgttg cccttcatta aggcgtaaat acatcgtgga ataaggcaac
    47941 gcctggatct gctgatccgc tacggtgata tcctgattat cgaaaaggct ggcgcgaaat
    48001 gtatcgacca tactttgctg gctgtgcgta catcccgcaa gaaatagtgc gaaaatcgat
    48061 aaaataagag ggcgcacctt taatccttga aaggttaatt aaaaaaatgc aaatctttaa
    48121 agcccggtac gaatatcgca accgggtatc atttaacttt aatttcgacg cgttaaagca
    48181 gtattagcgt gtagtggacg tggtggttga ggtgttatga gaaccgccat cgccactgtc
    48241 atttgctgca acaactgcca acgtcgccac accaacagcc gttgccacac cgacaactgt
    48301 cgccgtgccg acagcgccag cagccacgcc accagtggta gtagcagtcg tggttgcgcc
    48361 ggtagtggtc gttgtggtgc cagtagtacc cgtcgttgcc tcggtcccgt ttgttgcttc
    48421 aggggcagca aacgcagctg gcgtcgtcaa catgaaggcc ataaggattg cagaaagctt
    48481 gtgtttcata acttttcctt tattcatcgc atggacaata cgtacgcttg agatcaggat
    48541 tgtctacttt gagtataaca tccttgtgaa atctcccttt cctcataccg cttgcactat
    48601 atactgcggg taggaaaggc gcgcagaggg aaatataaga ttgttaacta tccccctctg
    48661 gaaatacgac ttgtgagcat aagataaatc ttagcaaaat acaaattaaa gataaggaaa
    48721 ttgttacgaa agctattaat tattgctggt aatatagttt caagtggtac tataactggc
    48781 tgttaaatgg catttataac tattaggtgc agagatattc gcttaaatgg attagtttct
    48841 ttgtgaaaaa gtaactgatg ttataatata tggtgttgac tattgcacta ttccaataaa
    48901 accagtcagc tttaaacaag cagcgtcata ttaagagaga taaacatttg ccgctgttgg
    48961 tcctcgcagg ccatttacgc ggcaaaattc cacacgtaat cctggtataa gcacttctgc
    49021 gtcgcgggga gtgaatgcgg aaatatggac ctgaacttct ttacgaccgt cggaggggat
    49081 aatgaatcct ttgccgcttt tgcgatcaaa ggttttgaca attcctgtca ttttacggga
    49141 caaacaaatt ccttactgaa aatactgcgc tgcactatac ggggttaata aaataaagcc
    49201 agcgatattt aagaccgccg gacggctaaa ataaaatttg cttaatatca attatcatgc
    49261 gttaatagct gcgtcggttt gaaagacaga cagcatacaa agtagtttac taaagcagtt
    49321 ctcattatca ggcattatcc ccttcttttg agtctctctc ctgaacacta agtagtttct
    49381 gtattaaagc cctgtttgcc gaaaggccca aaatgaagga agtaaaatat gtctaataaa
    49441 atgactggct tagtaaaatg gtttaacgca gataaaggtt ttggctttat cactcctgat
    49501 gatggcagca aagacgtttt cgtccatttc actgccatcc agagcaatga attccgcacg
    49561 ctgaacgaaa atcagaaagt tgaattttct attgagcagg ggcaacgtgg ccccgcggca
    49621 gcgaacgttg ttacgctcta aggttgccgt tattactcaa catctccatt tccgctgtcc
    49681 atgttgtcat ggttcacagt accgcacatc ggcattcgat gtgacggagc gaaatccttt
    49741 gggcgctaag tgtatttttt gtaaatcgac gatgatcacc tttgataacg tcgcgctgca
    49801 aatacgcact gaccatgcgc cgctggattt cacaaaataa tatcaggctc cctcg
//
LOCUS       DAJTNA010000008        48808 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTNA010000008 DAJTNA010000000
VERSION     DAJTNA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48808
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.008"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..156)
                     /locus_tag="OO161_000381"
     CDS             complement(<1..156)
                     /locus_tag="OO161_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR5461569.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVRMPY
                     SDEENSKM"
     gene            complement(282..587)
                     /locus_tag="OO161_000382"
     CDS             complement(282..587)
                     /locus_tag="OO161_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5461570.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(684..760)
                     /locus_tag="OO161_000383"
     tRNA            complement(684..760)
                     /locus_tag="OO161_000383"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(724..726),aa:Val,seq:gac)
     gene            complement(765..841)
                     /locus_tag="OO161_000384"
     tRNA            complement(765..841)
                     /locus_tag="OO161_000384"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(805..807),aa:Val,seq:gac)
     gene            1149..2405
                     /locus_tag="OO161_000385"
     CDS             1149..2405
                     /locus_tag="OO161_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5461571.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(2446..3819)
                     /gene="mdtK"
                     /locus_tag="OO161_000386"
     CDS             complement(2446..3819)
                     /gene="mdtK"
                     /locus_tag="OO161_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5461572.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAAGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            4034..4675
                     /gene="ribE"
                     /locus_tag="OO161_000387"
     CDS             4034..4675
                     /gene="ribE"
                     /locus_tag="OO161_000387"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5461573.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(4715..5863)
                     /gene="cfa"
                     /locus_tag="OO161_000388"
     CDS             complement(4715..5863)
                     /gene="cfa"
                     /locus_tag="OO161_000388"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5461574.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSDPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(6154..7365)
                     /locus_tag="OO161_000389"
     CDS             complement(6154..7365)
                     /locus_tag="OO161_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5461575.1"
                     /translation="MQPGKGFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAVQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRANVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRSEEAGCQNH
                     GNAEVAHSESH"
     gene            7478..8410
                     /locus_tag="OO161_000390"
     CDS             7478..8410
                     /locus_tag="OO161_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5461576.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(8407..9432)
                     /gene="purR"
                     /locus_tag="OO161_000391"
     CDS             complement(8407..9432)
                     /gene="purR"
                     /locus_tag="OO161_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5461577.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            9731..9820
                     /gene="ynhF"
                     /locus_tag="OO161_000392"
     CDS             9731..9820
                     /gene="ynhF"
                     /locus_tag="OO161_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5461578.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            9986..11155
                     /locus_tag="OO161_000393"
     CDS             9986..11155
                     /locus_tag="OO161_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5461579.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(11301..11882)
                     /gene="sodB"
                     /locus_tag="OO161_000394"
     CDS             complement(11301..11882)
                     /gene="sodB"
                     /locus_tag="OO161_000394"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5461580.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(12010..12825)
                     /gene="mepH"
                     /locus_tag="OO161_000395"
     CDS             complement(12010..12825)
                     /gene="mepH"
                     /locus_tag="OO161_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5461581.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            13159..13506
                     /gene="grxD"
                     /locus_tag="OO161_000396"
     CDS             13159..13506
                     /gene="grxD"
                     /locus_tag="OO161_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5461582.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(13557..17905)
                     /locus_tag="OO161_000397"
                     /pseudo
     CDS             complement(13557..17905)
                     /locus_tag="OO161_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(17998..18645)
                     /gene="rnt"
                     /locus_tag="OO161_000398"
     CDS             complement(17998..18645)
                     /gene="rnt"
                     /locus_tag="OO161_000398"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5461583.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(18748..19155)
                     /gene="gloA"
                     /locus_tag="OO161_000399"
     CDS             complement(18748..19155)
                     /gene="gloA"
                     /locus_tag="OO161_000399"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5461584.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGSVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(19236..20333)
                     /gene="nemA"
                     /locus_tag="OO161_000400"
     CDS             complement(19236..20333)
                     /gene="nemA"
                     /locus_tag="OO161_000400"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5461585.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELLSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(20370..20969)
                     /gene="nemR"
                     /locus_tag="OO161_000401"
     CDS             complement(20370..20969)
                     /gene="nemR"
                     /locus_tag="OO161_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5461586.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            21072..21311
                     /locus_tag="OO161_000402"
     CDS             21072..21311
                     /locus_tag="OO161_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5461587.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            21360..22256
                     /locus_tag="OO161_000403"
     CDS             21360..22256
                     /locus_tag="OO161_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5461588.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            22337..22858
                     /gene="sodC"
                     /locus_tag="OO161_000404"
     CDS             22337..22858
                     /gene="sodC"
                     /locus_tag="OO161_000404"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5461589.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(22859..24870)
                     /locus_tag="OO161_000405"
                     /pseudo
     CDS             complement(22859..24870)
                     /locus_tag="OO161_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(24870..25726)
                     /locus_tag="OO161_000406"
                     /pseudo
     CDS             complement(24870..25726)
                     /locus_tag="OO161_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(25792..25965)
                     /locus_tag="OO161_000407"
     CDS             complement(25792..25965)
                     /locus_tag="OO161_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5461590.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            26166..26600
                     /gene="slyA"
                     /locus_tag="OO161_000408"
     CDS             26166..26600
                     /gene="slyA"
                     /locus_tag="OO161_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5461591.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(26647..27114)
                     /gene="slyB"
                     /locus_tag="OO161_000409"
     CDS             complement(26647..27114)
                     /gene="slyB"
                     /locus_tag="OO161_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5461592.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            27388..28497
                     /gene="anmK"
                     /locus_tag="OO161_000410"
     CDS             27388..28497
                     /gene="anmK"
                     /locus_tag="OO161_000410"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5461593.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            28595..28924
                     /gene="mliC"
                     /locus_tag="OO161_000411"
     CDS             28595..28924
                     /gene="mliC"
                     /locus_tag="OO161_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5461594.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            28983..29639
                     /gene="pdxH"
                     /locus_tag="OO161_000412"
     CDS             28983..29639
                     /gene="pdxH"
                     /locus_tag="OO161_000412"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5461595.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLSLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDVWKIDRLAP
                     "
     gene            29768..31042
                     /gene="tyrS"
                     /locus_tag="OO161_000413"
     CDS             29768..31042
                     /gene="tyrS"
                     /locus_tag="OO161_000413"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5461596.1"
                     /translation="MASSNLIKQLQERGLVAQMTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            31101..31964
                     /gene="pdxY"
                     /locus_tag="OO161_000414"
     CDS             31101..31964
                     /gene="pdxY"
                     /locus_tag="OO161_000414"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5461597.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(32008..32613)
                     /gene="gstA"
                     /locus_tag="OO161_000415"
     CDS             complement(32008..32613)
                     /gene="gstA"
                     /locus_tag="OO161_000415"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5461598.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDDTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(32719..34221)
                     /gene="dtpA"
                     /locus_tag="OO161_000416"
     CDS             complement(32719..34221)
                     /gene="dtpA"
                     /locus_tag="OO161_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5461599.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(34832..35467)
                     /gene="nth"
                     /locus_tag="OO161_000417"
     CDS             complement(34832..35467)
                     /gene="nth"
                     /locus_tag="OO161_000417"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5461600.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(35467..36162)
                     /gene="rsxE"
                     /locus_tag="OO161_000418"
     CDS             complement(35467..36162)
                     /gene="rsxE"
                     /locus_tag="OO161_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5461601.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(36166..36786)
                     /gene="rsxG"
                     /locus_tag="OO161_000419"
     CDS             complement(36166..36786)
                     /gene="rsxG"
                     /locus_tag="OO161_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5461602.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(36790..37848)
                     /gene="rsxD"
                     /locus_tag="OO161_000420"
     CDS             complement(36790..37848)
                     /gene="rsxD"
                     /locus_tag="OO161_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5461603.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGS
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(37849..39975)
                     /gene="rsxC"
                     /locus_tag="OO161_000421"
     CDS             complement(37849..39975)
                     /gene="rsxC"
                     /locus_tag="OO161_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5461604.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAVPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVNED"
     gene            complement(39968..40546)
                     /gene="rsxB"
                     /locus_tag="OO161_000422"
     CDS             complement(39968..40546)
                     /gene="rsxB"
                     /locus_tag="OO161_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5461605.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(40546..41127)
                     /gene="rsxA"
                     /locus_tag="OO161_000423"
     CDS             complement(40546..41127)
                     /gene="rsxA"
                     /locus_tag="OO161_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5461606.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDKWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(41204..41644)
                     /locus_tag="OO161_000424"
     CDS             complement(41204..41644)
                     /locus_tag="OO161_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5461607.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            complement(41730..41945)
                     /gene="ydgT"
                     /locus_tag="OO161_000425"
     CDS             complement(41730..41945)
                     /gene="ydgT"
                     /locus_tag="OO161_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5461608.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(42218..42343)
                     /gene="blr"
                     /locus_tag="OO161_000426"
     CDS             complement(42218..42343)
                     /gene="blr"
                     /locus_tag="OO161_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5461609.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            42586..43626
                     /locus_tag="OO161_000427"
     CDS             42586..43626
                     /locus_tag="OO161_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5461610.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(43661..44662)
                     /gene="add"
                     /locus_tag="OO161_000428"
     CDS             complement(43661..44662)
                     /gene="add"
                     /locus_tag="OO161_000428"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5461611.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(44766..45938)
                     /gene="malY"
                     /locus_tag="OO161_000429"
     CDS             complement(44766..45938)
                     /gene="malY"
                     /locus_tag="OO161_000429"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5461612.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQERVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(45948..47534)
                     /gene="malX"
                     /locus_tag="OO161_000430"
     CDS             complement(45948..47534)
                     /gene="malX"
                     /locus_tag="OO161_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5461613.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVSIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHGLS
                     TKWYMVSVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGYNV
                     PAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQVVI
                     GPQVQSVKDEMAGLMHTVQA"
     gene            47709..48737
                     /gene="malI"
                     /locus_tag="OO161_000431"
     CDS             47709..48737
                     /gene="malI"
                     /locus_tag="OO161_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5461614.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     CKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT        12353 a        12281 c        12680 g        11494 t
ORIGIN      
        1 catttttgag ttttcctcat cactgtatgg catcctgaca ccggcctcgt cagcctgctc
       61 gaagatagaa atagaaagtg gagtttgttg ctgtagaagc gagaataagg tgtagatccc
      121 cgtaggcccg gcacccacaa tagcaatttt tttcattgtt acttacctgt acgcattctt
      181 tcgaatttag tgttatcaga taagtttggt tgagattttt agaatttgag aaatttcaga
      241 cagggatgaa aagggcgcta accgcccttg attaaattct gtcaggcgag ttttgcctga
      301 ttgatttgac tcagtggctc attgacatcg aaaactttgg cgacaacttc ctcaacgccg
      361 agatttttaa gtcgggcagt atgtttttcc agataggcaa gggcgctttt ttcatcagta
      421 aaaagataga tcccaccggc ttcgtggttc ttttcacttt ctgtccatac cttccacaga
      481 aaaccaggtt cctgattaat cgattcagca agcggtttaa gctgctcagc cattgcgtcg
      541 ccaaatgggc cattaaaagc aaaatgaagt tgtaacaaag ttgccatagc gattctcctt
      601 ctgataacgc atacgaagac agtaacgtat tggatacaga gaagaaacag agggaaaaag
      661 gagaagacca gattaagaaa atctggtgcg tccgagtgga ctcgaaccac cgacccccac
      721 catgtcaagg tggtgctcta accaactgag ctacggacgc aggatggtgc gttcaattgg
      781 actcgaacca acgaccccca ccatgtcaag gtggtgctct aaccaactga gctatgaacg
      841 caacgttgta ggtgacaacg gggacgaata ttagcggcag agtgggaagg tggcaagagg
      901 caaaacgtaa ttttctgcgc tatttcgacc gtttgcagag cttttaagca aattggctat
      961 attttgttga tttgcaaggg cgatttttat tcaggatcgc atttacatct gatgcaaccc
     1021 aataattcgt gtatcagagt ttaaacagag ttgtttgcca ccatctcaaa tctgtcgtgg
     1081 ataaattctc cgtagccttt acacttaggt agcaataaag gcacacagat gcagcaaagg
     1141 aataaatcat gggatctgat gcgaaaaact tgatgagcga cgggaatgtg caaattgtta
     1201 agaccggcga ggtcattggc gcgacgcaac ttactgaagg cgagttgatt gttgaagctg
     1261 gcggaagagc cgaaaatacc gtggtcacgg gggctggctg gttgaaagtg gcaaccggtg
     1321 ggatcgccaa atgcacacag tacggtaaca atggcacgct atcggtcagc gacggtgcca
     1381 ttgccacaga tattgttcag tccgagggag gcgcaattag tctctctacg ctcgctacgg
     1441 ttaatggccg ccatcccgaa ggtgaattca gcgttgatca gggttatgcc tgcggtttgt
     1501 tgctggaaaa tggcggtaac ctgcgtgtac tggaaggaca tcgcgcggaa aaaatcattc
     1561 tcgatcaaga gggcggcctg ttggttaatg ggacaacctc agcggtcgtg gtagatgaag
     1621 gtggtgaatt gttggtgtat ccaggtgggg aagccagcaa ttgtgagatt aatcagggcg
     1681 gcgtttttat gctggccggg aaagccagtg atacgttgct tgctggtggc accatgaata
     1741 atctcggtgg tgaagactct gacactattg ttgagaatgg atccatttat cgtctgggga
     1801 cggatggcct tcagctctac agttccggta agacgcaaaa cctgtccgtg aatgtgggtg
     1861 gtcgggctga agtgcatgcc ggtacgctgg aaaatgcggt aatacaaggt ggaacagtga
     1921 tcctgttgtc acccaccagc gcggacgaaa attttgtcgt agaggaagat cgcgcaccgg
     1981 ttgaactgac cgggagtgtt gcattactgg acggcgcttc aatgattatt ggctatggcg
     2041 cagatctgca acaatcaacg attactgtac agcagggcgg tgtgttgatt ctcgacggca
     2101 gtacggtaaa aggtgacggt gtcactttta ttgttggtaa catcaatctg aatggcggaa
     2161 aactgtggct gatcactggt gcggcaacgc atgtgcaact gaaagtgaaa cgcctgcgcg
     2221 gagagggagc gatttgcctg caaaccagtg cgaaagaaat ctcacctgac ttcatcaatg
     2281 tgaaagggga agttaccggt gatatacacg ttgagataac agatgccagt cggcaaactc
     2341 tgtgcaacgc tctgaaatta cagccagacg aagacgggat tggcgcaacg ctccagcctg
     2401 cgtaaaaaca caggcgcaac ccgtgaaggt tgtgccttgt ctttattagc gggatgctcg
     2461 ttgcagaatg ataactgacg gcagacgttg caggaaccgc atacgcaaca tcatcataat
     2521 ggctgccgat gtcaggccga taataaagcc tatccagaag cctgctggcc ccataggttc
     2581 aacgaccaga tcggtcagtg ccagaatata gccgcttggc aagcccagca cccagtaagc
     2641 tgtaaaggtg atatagaaaa tggaacgcgt atctttataa ccacgcaaaa tcccactgcc
     2701 aatcacctgg attgagtcag aaatctgata taccgccgcc agcaacatca aatgcgcagc
     2761 cagcgttaca acctcgggat tgtcgttgta caacagggcg atttgctccc ggagtgaaac
     2821 cgtgaaaatg gccgtcaggg ttgccataca gacacccacc ataagcccgg tccgcgcagc
     2881 ggtttgcgca tccagcgttg agccctgacc cagacgataa cctacgcgga tagttaccgc
     2941 tgccgccagc gacattggaa gcacaaacat cagtgaacta aagttcaggg caatctggtg
     3001 tcctgcgaca tcaacaatac cgagcggaga cactaacaga gccacgacgg caaacagtgt
     3061 cacttcaaag aatagtgcca gcgcaatcgg taaaccgagt tgaatcagtc gtttcataac
     3121 cgcaggatcg ggtttcgcgg tgcctttttc gttacgaata tcgcgcatgg agcgggcgcg
     3181 tttaatgtaa gaaaccatgg caaggaacat gacccaatac accgccgcag tagccacgcc
     3241 acaaccaacg ccaccgagct caggcatacc gaaatgacca taaataaaga tatagttcac
     3301 cggaatgttc accagcaggc cgataaagcc cattaccata cccggcttgg tttttgccag
     3361 accttcacat tggttacggg caacctggaa gaacagatag cccggcgcgc cccacaacaa
     3421 cgcacgcaga taacccgcgg ctttgtccgc cagagccgga tcgatgtttt ccatggagcg
     3481 gataatgtaa cctgcattcc acagcaccag cataatgaga acggaaacaa aacctgccag
     3541 ccagaaacct tgtcgcacct gatgcgcaat gcgctcacgt cgaccggaac catttaattg
     3601 cgcgataacc ggcgttaatg ccagcagcag tccgtgacca aagaggatcg ccggaagcca
     3661 gatagaagta ccgatagcga ccgccgccat gtcggtggca ctatagccgc ccgccatcac
     3721 ggtatcgaca aaacccatcg cagtttgggc gatttgcgcg agaatcaccg ggattgctaa
     3781 tgctaataac agacgcgctt cactgatata cttctgcacg tgaacacctt ttatttgtag
     3841 ttatatgaga gactaaaaag ccgcctgaat gggcagcaaa aagaagaagc aggggaaatt
     3901 cagtctattg tagcgaggat ttattaattc tccagtgaaa aaatagtcat tcttacccct
     3961 tcactggtaa gggcgtttgc cacctgctat tgtgctgaac agaatgtctt aactgatttc
     4021 aggagttgta agtatgttta cggggattgt acagggcacc gcaaaactgg tgtcgattga
     4081 cgagaaacca aattttcgta cgcatgtggt ggagttaccc gaccacatgc tggacggcct
     4141 ggaaaccggt gcttccgtgg cgcataacgg ttgctgcctg accgtgacgg aaattaacgg
     4201 caaccatgtc agttttgacc tgatgaaaga aacgttacgc attaccaatc ttggcgattt
     4261 aaaagtgggg gattgggtaa acgttgagcg tgcggcgaaa ttcagtgatg aaattggcgg
     4321 acacttaatg tcaggtcata ttatgaccac cgctgaagtg gcgaaaatat taacctcgga
     4381 aaataatcgc cagatctggt ttaaagtcca ggatagtcag ttgatgaaat atatcctgta
     4441 caaaggattt attggcatcg acggtattag cctgaccgtc ggcgaagtca cgccaacgcg
     4501 tttttgcgtc catttaattc cggaaactct ggaacgcacg actcttggga agaaaaaact
     4561 cggcgcacgc gtcaacattg aaattgatcc acaaactcag gcagtggtag atacggtaga
     4621 acgtgtgctg gcggcacgag aaaatgccat gaatcaacca ggtacagaag cctgatctga
     4681 aatcgccccc ggagaggggg cgatagaata gcctttagcg agcgactcga aggccgtttt
     4741 caacaccgcg tgagaacacg acctgccaga gctgaatatc acgggcgcgg aaagcacctg
     4801 cgcaggcatt cagataatag gtaaacatcc gtttaaagcg ttcactgtaa ttatccgcaa
     4861 tttctggcca tgcggcgagg aatcgttcat accacgccat caacgtagta tcgtaatcag
     4921 caccgaagtt atgccagtct tccatcacaa agtgggggtc gctggactga gcaatctggc
     4981 gtacagaggg caggcaaccg ttcggaaaaa tatatttatt aatccaggga tcaacattca
     5041 gatcggtttt tttcgaaccg atagtatgga gcaggaatat gccttccggt ttcaaattac
     5101 gatccaccac cgcaaaatag gtatcgtaat ttttcggtcc gacgtgctcg aacatcccca
     5161 cagaaacaat acgatcaaac tggtcgttca ggtcacgata atcttgcagc aaaatggtga
     5221 catccaggcc ttcacagcgt tcctgagcca ttttttgctg ttcggcagaa atggtgacgc
     5281 ccaccatgct tacgtcataa ttagatgcca tgtagtgtgc cagtccgccc cagccgcagc
     5341 caatatccag tacgcgcatc cctggtttta actgcaattt ttcacaaatc attttgagct
     5401 tcgcctgctg ggcagattcc agattatcgg catctttcca gtaagcgcag gaatattgca
     5461 tgaagggatc aagcatgcgg ctgaacaagt cattacccaa atcgtaatgc tctttgccga
     5521 ctatccaggc acgtttttta ctctgcagat tgaagagacg agcgccggca atacgcagcg
     5581 tgtctttgaa atgatggggg agttggttct cgagacctgc gcgtaagact ttgctaaaaa
     5641 acatatccag tcggtcacat tcccaccagc catccatata actttcgcct aaccccaaag
     5701 agccttcttg cagaacgcgt ttaaaaaaat cggggttttt cacacgaata tccgccgggg
     5761 cagaaccgtt aatggctata ccggcacggc taagtaattc gttggcgata cggtaccagt
     5821 tgtcatccgg tacgctgact tcttctatac acgatgaact catagtttct ccatcactgg
     5881 tcggtgatca gaaccttaaa acagcgtaga cgctttttgg gctttgtgag aaatctcgca
     5941 gaaaaaaccg cgagtctata atccgacgta gaacagaggg aaaggaggaa cccttgccga
     6001 aatgaccatc catagtgagt cgggaacgat cctgtccccg taaaaacata atatttatcg
     6061 tagctttaac aactcgaaat tagtataggc cgctaatttc aatcatacaa caaaattttg
     6121 ttagcagcct aagtataagt atatcgatat agatcagtgt gattcgctat gagcgacttc
     6181 ggcattgcca tgattctggc agccagcttc ttcactacgt tgcatcatgt aacccagcgc
     6241 gaccagcact actgttgata acatcacgct ggtggtggtg agcaatggcg tgctgatact
     6301 aataagccag gaaactacca gacttgcgag gaagcacaga cccagttgta gagtgttctg
     6361 caacgctgcg gcgcgaccag ttgcgtgtgg gaagggacgc agcgcctggg cgacaacaat
     6421 agggtagatc gcgccattgg caatcgccat cacacagaat gggatcagga tttcgaccag
     6481 cgacacatgg ctaatgaagc ccgcagccca ggtcgcaatg acgctgacag caaacagcac
     6541 cagcaaccac ggtaataact gcttgccttg ccatttctgc agcgcggcgc gacagccata
     6601 accaccaatc agaaacgcga tagtttgcgg gacataactt aaaccaataa ctgccgggct
     6661 gtagcccatt tcactaagga tgaacggtga accggtcagc catgcaaaaa aactggctga
     6721 acaggccgcg tatatcagca cgttggcgcg ataggtttta gaacgtagca ggtcggtaaa
     6781 ggtcagacca tcctgactat tgttaccggc cttcgtcgtg ggtttgagcc agaaaatagg
     6841 cagaatcagc accacggtaa tggcaaacag ggtggcgaaa atcgcctgcc aggaaaaatg
     6901 gaccagcagc cagcttccta acagaggagc cagggccgga gatagaccca ccagcggcat
     6961 gatggtcgca aaaattcggt taactttctg tgaaggataa taatccgtca ctaacgcttg
     7021 ccagataacc gccgcggcgc agacacccac agcctgtaca aaacgcaata ccagcagcgt
     7081 agcggcgttt tctacccaca gcatccccag actacctaac gcaaaaattg tcaggccgat
     7141 taataacacc ggtttacgac cataacggtc ggagagtggc ccccacagaa gctggactgc
     7201 ggcaaaaccg gcaaggaaca gactaaggct ggcactgaca gcagacgcag gcgtttgcag
     7261 gtcggcctgt atggcggcga aagcaggcag atacatatcg gttgccagaa aaccgagtac
     7321 gctcaaaccc gccagccaga ctaaaaatcc tttcccaggt tgcatgtata ttctctaaat
     7381 ctctcaatta aacaccgccg caaagtgtag ggagtgaaat tagtgttgtg aaacggtaat
     7441 atttgatgac tgatttcaaa aattttgcag gcagactatg tggtcagaat attcactcga
     7501 agttgttgat gcggtagcgc gtaatggtag ttttagcgct gcggcacagg agctgcatcg
     7561 cgttccttct gcggtcagct ataccgtgcg tcagctggaa gagtggctgg cggtgccgct
     7621 ctttgaacga cggcaccgtg atgtggagct gaccgctgct ggagcgtggt ttctcaaaga
     7681 agggcgctct gttgtcaaaa aaatgcagat cacccgccag caatgtcagc agatagcgaa
     7741 cggctggcgc ggtcagttag ctatcgcagt ggataatatt gtcaggccag aacgtacacg
     7801 gcagatgatc gttgattttt atcgccattt tgatgatgtc gaacttcttg tctttcagga
     7861 agtgttcaac ggtgtctggg atgcgctttc cgacgggcgc gtggaactgg cgattggcgc
     7921 aacacgcgcg attccggtag gcggtcgtta tgccttccgg gatatgggga tgctaagctg
     7981 gagttgcgtt gttgccagcc accacccgct ggcgttgatg gatggcccat tcagcgatga
     8041 tacgttgcgc aactggccgt cgttggtgcg cgaagacacc tcgcgaacgc tacctaaacg
     8101 tattacctgg ttgctggata accaaaaaag agttgtcgtg cctgactggg aatcatcggc
     8161 aacctgtatc tcggcaggat tatgcatagg gatggtgcca acacatttcg ccaaaccgtg
     8221 gcttaatgag gggaagtggg tagcacttga attggaaaac cccttcccgg attcggcatg
     8281 ttgtctgaca tggcagcaaa atgatatgtc gcctgcgtta acctggctgc ttgagtatct
     8341 cggcgatagt gagacgctga ataaggagtg gctgcgggag ccggaagaga ctcccgcaac
     8401 gggtgattaa cgacgatagt cgcggaacgg gccgtcagcc acggagcggc gttcaatcaa
     8461 gcgcggatgc acttcaatgg actgcggttc ttcacgtttg ttgacgatac gatccaacag
     8521 catgttgaac gctgtttcac ccagcgaatc ttttggctgg tggatcgtgg tcagcgccgg
     8581 cgtaaaatag cgggcgttgc gcacgttatc ataaccgatc agcgaaacat cctgcgggac
     8641 gcgcagaccc atttcatcag cagcacaaag tgcgcccatt gccatgatat cgccaccaca
     8701 gaagacggca gtagggcgat gcggctgcga cagtatttgc tgcatggcgc gataaccaga
     8761 ttcgggttca aagtcaccct gaacaatcca gctttccggc accttgatca tcgcttcttc
     8821 catcgccttc ataaaaccgg caaggcggcc tgcgccggtg ttacgttcca gcgggccggg
     8881 gatgacgccg atttcgcggt gaccgcgttc aatcagataa cgcccggcca tgtagccgcc
     8941 ttcgaacgcg ttatcaatga ccgcatcggt gaagtcagct tttgcttcac cccagtccat
     9001 caccaccatt gggatatggc gatactcttc cagcatcgcc agcaacggct ctgggtactc
     9061 agaacacatc accagcagac catcgacgcg tttttgcgcc atcatcgaca gataagcccg
     9121 ctgtttctca agattgttcc acgcattacc cagaatcagg gtgtaacctt tctggaagca
     9181 atttttttca accgcttcaa tgatctcggc aaaataggcc gcttcgctgc tggtcgccag
     9241 caaaccgata gacttggtgt ggttaacctt caggctacgc gccaccgcgc taggggagta
     9301 gtgtaattct ttaatcgctg cccacacggc gttgcgcgtt tcttcagcga cgaaacgtgt
     9361 tttgttgatt acgtgtgaca cagttgtagt ggaaacgttt gctcgtttcg ctacatcttt
     9421 tattgttgcc attccatttc actccagacc ctaacttcag ctcctgcaaa atcgcaaggt
     9481 aaacgtttgc ctttacacac cttacgcaca acaaaagtgt tgcggtacgc cggaaaaacg
     9541 acacggagcg tcaggaaggg gtcaatggcc agtacgcaaa tagtgtaagc gtggaatttt
     9601 ggccgatctt gacgaaaagg ggaagtggta aaaacactta tcattataaa tgacgggaga
     9661 ttttttcatc acagtgtgta aaaatgcgta atatcactat ttgtgtggta ataaaaaagg
     9721 agaaaacttg atgagcaccg accttaaatt ttcactggta acaacgatta tcgtcctcgg
     9781 tttgatcgtg gccgtgggtt tgactgccgc gctgcactga tttcctctcg agggagcgcg
     9841 ttctccctcg catctccttc ctgtttgtta gcaaaatggc aaaagtgttt tctctttttg
     9901 ttattttcat ttttttgtga ttgctgtcat gcttttgcca ctttaatgaa tatcgcgtaa
     9961 tgacgcgaag aggtggagat aaggcatgaa aattaactat ccgttgctgg cgctggcgat
    10021 tggcgcgttt ggtatcggga caacggagtt ctcgccaatg ggcttgttgc ccgtcattgc
    10081 gcgcggtgtg gatgtctcga ttcccgctgc cggaatgtta atcagtgcct atgcagttgg
    10141 cgtaatggtt ggcgcgccgc tgatgacgct tctactttct catcgtgccc gccgcagtgc
    10201 gttgattttc ctgatggcaa ttttcacgct cggcaacgta ctttccgcca tcgcgccgga
    10261 ttatatgacc ctgatgcttt cacgcatttt gaccagcctg aatcacggag cattttttgg
    10321 tttgggttca gtcgtggccg caagcgtgga gccaaaacat aaacaggcca gcgcagttgc
    10381 cactatgttt atggggttaa ccctggcaaa tatcggtggc gtgccggcgg cgacctggtt
    10441 gggtgaaacc atcggctggc ggatgtcatt tctggcaacg gcggggctgg gagtgatttc
    10501 aatggtaagt ctgttcttct cattacctaa aggtggtgca ggggcacgac ctgaagtgaa
    10561 aaaagagctg gcggtattaa tgcgtccgca ggtgctgtct gcattgctga cgacggtact
    10621 gggagctggt gcaatgttta ctctctacac ctatatctct ccggtactgc aaagtattac
    10681 ccacgcaaca ccggtgttcg tcacggcaat gctggtgctg attggtgtcg gattctctat
    10741 cggtaactat cccggcggca aactggcaga tcgttcagtt aacggcacgt tgaaaggctt
    10801 tttgttgttg ttgatggtga ttatgctggc aatcccgttc ctggcccgca atgagttcgg
    10861 cgcagctatt agcatggtgg tgtggggcgc agcaaccttt gcggtcgtac cgccgttaca
    10921 gatgcgcgtg atgcgtgtcg ccagtgaagc gccaggtctg tcttcatcag tcaatattgg
    10981 tgcctttaat cttggaaatg cgctgggagc agctgctggt ggtgcggtaa tttccgctgg
    11041 gctgggatac agctttgtgc cggtgatggg agcgattgtc gcgggactgg cattattgct
    11101 ggtgtttatg tcagccagaa aacaacctga aacagtttgc gttgctaaca gctaattaaa
    11161 caaaaacgca gaaggataac cttctgcgtt acagttgctg acaacgtgcg atttatcttg
    11221 ccgaatgcgc tgttaacgcc tcatccgcgt tggcatggta aacgaagcgc atcaggcaat
    11281 gctgcatttg ccataagtta ttatgcagcg agatttttcg ctacgaattc ccagttcacc
    11341 agcgcccaga agtgctccag atagccagga cgtgcattgc gatagtcgat gtaataagcg
    11401 tgttcccaga catcaacggt cagcagcgga gtcgcatcgg tggtcagcgg agtacccgcg
    11461 ttagaggttg aaacgatagc cagtttgcca tcgctgtttt tcaccagcca ggtccagcca
    11521 gaaccaaagt ttttgatcgc tgcatcagta aactgcgctt tgaaatcggc aaagctgcca
    11581 aaagatgcgg cgatagcttc agcgactttt ccagtcggtt cgccaccggc gttcggtgcc
    11641 aggcagttcc agtagaaagt atggttccag acctgagctg cgttgttgaa tacgccacct
    11701 tcagagctgc gaataatctc ttccagtgat ttaccttcaa acgcggtacc tttaatcagg
    11761 ttgttcaggt tagtgacata agtctgatga tgcttgccgt agtgatactc gatagtttcc
    11821 gcagaaatgt gcggtgccag agcatcttta gcatatggta gtgcaggtaa ttcgaatgac
    11881 attgctactc tcctttatta ttaatttgca tacgtacaat agccttattg tgcgtatgga
    11941 tagggtagca aattaaaagg tgagacaaaa gatgaactta ccctgttgcc gtaacaacag
    12001 ggtaaagttt tagcgaagtg tttttggggt cattacccga cgagcgccaa catagtggcg
    12061 ctgccagtag tcttcactga gagaagtgat ttgaatttcc tgacctgtgc gcggtgactg
    12121 aataaatttg ccgttgccga catacacgcc gacatgatcg gctgtgccgc gtccctgagt
    12181 acggaaaaag accaggtcgc cgtttttcag ttcactacgt tcgattggcg ctgcatcacg
    12241 caggtgatac atttcattcg ccgtacgcgg aatacgaatt ttcaccaaat ctttataagc
    12301 gtaataaacc aggccgctgc aatcaaaacc ggtacgcggt gagctgccac cccaacgata
    12361 tggcttacca atttgctgca tcagtttatt cattgccact ttcgtagctt tttgcacctt
    12421 cgctttgtgc gcgtcggcaa gagttcctga cgcggcattt ttgactttca cacaatgcga
    12481 tttatagccc ttacgtttgg tacacttttc ggtaaagctg gctgaggccg ttttattgac
    12541 ggcgtgtttg ctgcgagttt tggcgttttt agaagcggta atggaagatt ttttaacgga
    12601 agaagcggtt ttgcttttgg tcgtactttt actggcggct tttttcgctg ttttctgggt
    12661 ctttttggtg gttgccgtgc ttttcttttt atctgctttt ttggtgatat gagtggtagc
    12721 agaactctcc ctggcttgct ttgaagcatg ggccataggc gttaaaggca gggtggtaaa
    12781 aagtaaagca cagagcgtga tcgaaatacg gtttatccgc gccactgagc gtccctctgt
    12841 taaggcaggt ctatcatgcc tgcgtctgat ttacaaaact tgatgattct aatgcataga
    12901 agaagagaaa gttaatagct tttattgtta aaaaatacgt ttcgttggcg agcgcaaaaa
    12961 aatgcagcag tgaaagctaa aaatggttat aaatacgcca gtttgccggc atcagagaag
    13021 aaacgaatat ttgaaatgca acaaatagag caaagggata aaatagcaaa agcgtgatga
    13081 aaatgttatt ttccgatgcc gcactgaccc gctacaatgt cagattactg ccgtaaagaa
    13141 gtttgaggaa gcaagacaat gagcaccact atcgaaaaaa tccaacgcca gattgctgaa
    13201 aacccgatcc tgctgtacat gaaaggttca ccgaaactgc cgagctgcgg tttctctgcc
    13261 caggcagtcc aggcgcttgc cgcatgtggc gaacgttttg cctatgttga tattctgcag
    13321 aatccggaca ttcgtgcgga actgccgaaa tatgctaact ggccgacctt cccgcaactg
    13381 tgggttgacg gcgagctggt cggcggttgt gatatcgtga tcgaaatgta tcagcgtggc
    13441 gaactgcagc agctgatcaa agaaactgcc gctaaataca agtctgaaga gccggacgcg
    13501 gaataatccg tcctgcaata ctgacggatg cgaacgggca tccgtcagtc ctttctctat
    13561 ccccaatcca gcccttgtgg cgaacttgaa aatcccgcct catgcagcag cgtaaacatc
    13621 ggcgtttgcc ggaccggtag atcattcact tctgttagcg taaagcgcag ccgtggttcg
    13681 cgacgcagtg cggtagtcag cgcgtggaaa acctctgggg cgagtaattc ctctttttcc
    13741 tgccagacca gcattttttt gccaccttgc gccagataga gtaacaattt gccgctagaa
    13801 acgaccacca gcgcacccgc ccgacgcgtt ggaaccagcg ttgccggatg tgcaggccag
    13861 ggcagaagat ttccccacac attagccgga tcgttggcag agagcgccac tggtgtatag
    13921 tggcgcgttt gcgtggcttg tgtcgccaga tcgcgccatc ggtcaatagt cagacgttca
    13981 gcgaattgcg cgccacccag accttctaca aaacgacctc gcataattcg cccggagtct
    14041 tccatacttc gacaaagcgt ttgcatcgat ggaaacccgc cagggatatt ttcggctatc
    14101 accgcctgac gactgatgat gccgtagcag tcgagcatgt tttccgccag cgccagcatc
    14161 ctttcggtat cgtttagtgg ctccacctgc aataacgacc agcgtccagc cagatttggt
    14221 gtgttgtaag ataccagcgc gagacgggtt gcgcatagac aggacgtcca cgacgagccc
    14281 ggtgactgcg gcgagttgag gtgcgtgcgt tggaactgct gcgggtgagg gcgcgtaacg
    14341 gtgcccaaat gtcgctggtg atgacgcctt gccataccag cgcccataac gcctcttgca
    14401 gggcagagag atcaaccgat tcgccaattt tgtcgcgtat ccgttggctg atttgttgtg
    14461 caaaccaggc tcctccgtca gccagaacag cgactatcgc ttgttgcagc gccgaacgat
    14521 tcgcctgatc cgcttcggcg ggagtgaacg attctgcagc atattcctgt agatgcagtg
    14581 ccaccaggcc gtcatcttca cccagttttt tttgccccga ccagataacc gcaccggttg
    14641 ccagtaattc atcgagcatt tctgacgaat agtcgcgtac gcgggcaggc aggatctggc
    14701 tttcccagag tgaggcgggt aatacgccct gacgttccag cagcaatcgc gcataggtcg
    14761 tggctgcaac gggacgcgtc gcttctctgg cggcttgcag cgagcgcaaa cgcagacgac
    14821 gaaatacttc atcgctcacc cagatgtctt gttgcagatt catcaccaga ccctgttcac
    14881 gcagttgctg aagctgctct tcgacaatgg caataccgag actaaactca tgagccagtt
    14941 gttcagccgt gaccagagca tgagcccgga gatagcgcag aaagaggtcg cgaagcgggt
    15001 aactgactct atgtaaataa atctctggca atgactctgg tagtcgtacg ccgagggcat
    15061 cacgcagcct ggcggcatca tccatacagg caagacgctc ctgtccgcta atcatcgctg
    15121 ggaagattcg ttttactgca agaagatttt ccagatacga cgcaacctct tcactgctgc
    15181 ctgtatgctg ttgcgccagg tcttcaacgg tcattggccc cagttcgcgc agcaggtcga
    15241 acaatccttc ttcacctttc gctcttctgc caggagccag tcgttgcaac tcttcttcca
    15301 cctggcgaat gacctgcggg tcgagtaatt cccccggatc gacctgtccg agtagattgc
    15361 gcagtaactc gctgtccagc gacagtacgg atgcccggcg ctctgccagc ggggcgtcgc
    15421 tctggtacat aaattccgcg acatagccga acaataaact tgtggcgaaa ggcgagggcg
    15481 tagtggtcgt tacatcggat atttgaattt cgccaccgtt caggcgacgc atcaaacgtt
    15541 ccagtgcggg aagatcataa acatcttgca gacattcgcg taggatttcg agaatgaccg
    15601 gaaaatctgg atatccctga gcgatttcca gcaactgact ggcgcgcagc cgttgttgcc
    15661 aaagcggggt gcgatggccc ggagtgcgcc ccggcattaa taatgcccgc gcggcgcatt
    15721 cgcgaaaacg ggcggcgaaa agtgccgagc tgcctaccgc ctcgcggaca atttgcagca
    15781 acttttcggg ttcaaacaaa aaaatcgcgg cgtcgggcaa tttaccatca gtgtcaggaa
    15841 tgcgcgcaac aatgccgtca tcactggcga ccacagacgc gtcagcgccc catagggcat
    15901 gtattcgccc ggcaatcgcc agcgcccagg gttcatgcac ccgtcttcca tagggagagt
    15961 gcaaaataat acgccagtcg ccaatttcat cacggcaccg ttcgaggacc agatggcgac
    16021 tccccggaac gatgcccgtc gcgttgcgtt gctcgtcaat caacccctga atattggcgt
    16081 tcgtattttc ttctgccagc cacgggggaa tagtcccgga aaagaacgcg ccatccgcca
    16141 gtaaatgaag aaaatcgccg atcatctcgc ctaattcagc cggacgtccg ttaccttcac
    16201 cacgccagaa ggggagccgg gcagaacgac ccggagcagg agtcacaatc acctgatcgc
    16261 gggtgatttg ctggatccgc catgaggtag cgccgagagt gataatgtcg ttcacccgcg
    16321 actcatatac catctcctca tccagttcac ccacccgccg cgaaccggcc ttttcttcac
    16381 cttcgggtaa taacacgcta tacatgccac gatccggaat ggtgccgccg ctggtaaccg
    16441 ccgacaattg agcgccaggt cgggcggtca atatcccggt ctccctgttc cagaccagtt
    16501 tggggcgaaa agcagaaaaa tcgccagagg gatagcgccc ggaaagcata tccagcgtgg
    16561 cgtcaaaaac acgtcttggc agatctttcc acggtgcggc acggcgtacg cgggagtacc
    16621 attcgtctac ctgtaatgcc tccatcgccg cggcggcaac ggtttgctgc gcaaggacgt
    16681 cgagtggatt atgcggcggt gtcaggtttt ccagcctgcc tgcgaacata cactctacaa
    16741 tgactgcgga atcgactaag tcacgccggg tacgagggaa aaacagccct ttagatacac
    16801 cgccaacctg atgtccggcg cgaccaatgc gttgtaaccc actggcaaca gaaagcggcg
    16861 ttgccacctg aatcaccaga tccactgcgc ccatatcaat ccccagttcg agactggagg
    16921 ttgcgaccac gcagcgtaac tcacccgatt tcagcgcctg ctcggtgatt gctcgttgtt
    16981 ctttagagac ggagccgtgg tgtgagcggg caataaaaac gtcgctactt tgtacacggt
    17041 tagaggttgc gccggaggtc gactcgaaat gggccgcatc aacggcgata gacggggaac
    17101 gctgtaagcg tgcggcgtaa agctcattta atcgtgccgt cagtttttcc gccagcccac
    17161 gtgaattagt aaagacaatg gtcgagcgat ggcgcaacac ttcatcaagg atacccgttt
    17221 caatatatgg ccagatggag ccttcccggc cggcatggct gtcttcgccg gtgctgctgg
    17281 cgaccgatga gacatcatcc atattggcga ccggtacgac aattcgtatc tgcggatggc
    17341 gcattgcggg cgggttgact accgtaaccg ggcgatcgcc accaagaaag gctgccacat
    17401 cgctggctga gcgcacagtg gcagaaaggc caattcgctg tgctgaggtg tggagcagcg
    17461 catcgagccg ctccagactt aacgccagat gcgcaccacg tttactgccc gctaccgcgt
    17521 ggacttcatc aataattacc gtttcgccgc gccgccgccg ttcatcggca atacccttca
    17581 acgggagctg caagttgcgc tgaacgtcgg tgcccagagc ttttatcggt gaaatataga
    17641 ggatgcgtga ggttttacgc ttatgcgcct cgcgggtatc ttcgccgcct tcgcggaaga
    17701 gccgatcgag ggcgtagaga aatgctgcca gcgttttccc ggagccggtc ggtgcaatca
    17761 ccagcgcatg ttcgcttcgc gccgccacat gccaggtttg cgactggaca gcggtcggct
    17821 gtttaaaggc gcgaagaaac cagtcgcggg tcgccggtga aaacacgtcc ggcaggagcg
    17881 atgaagggtc tggattatct gccattaacg ctattccccg tgaatatcat gcaggatgcg
    17941 tggaatagcc tcactataga tgcctgaatc attgcatgtc atcaggcatc gactcgatta
    18001 cacctcttcg gcggcaggta gcggccagcc tcccagacgt ttccagcggt tgacgatttc
    18061 acaaaacagc acagcagtgc gttcggtgtc gtacagcgcg gagtgcgcct gggtgctgtc
    18121 gaagtccatg ccagcggtct ggcaagcctt tgacaatacg gtttgtccga gtgccagccc
    18181 ggccagtgca gcagtgtcaa aagtggcgaa agggtggaac gggttacgtt tcagtgaggc
    18241 gcgttctgcg gcggccatca taaagctgtg atcaaaattg gcattgtgcg ccaccataat
    18301 ggcgcggtta cagccgctcg ctttaatacc tttacgtaca actttaaaaa tttcgtgcag
    18361 cgcctcgtat tcgctgaccg cgccgcgatc gggatcgttc gggtcaatgc cgttgaaggc
    18421 gagggcttct ggttgcaaat ttgcgccgac aaatggttcg acgtggaaat gtaatgtggt
    18481 gtccggcatc agccagcctt gttcatccat tttcagggtg atggcggcaa tctcaagcag
    18541 cgcatcggtt ttggcgttaa atccggctgt ttcaacatcg atcacaacag gataaaaacc
    18601 acgaaaacgg tcgcacagac cggtaagttg agcgttatcg gacatctgat tctcttaata
    18661 cggataaatt gcagcgcgca ttatgacaaa tattacttgt cgatgcagta aagatgcggg
    18721 cgcgatgagt tcacgcccgg caggagatta gttgcccaga ccgcgaccgg cgtctttctc
    18781 ttcgattaac tcaattttgt aaccgtccgg atcttccata aacgcgataa ccgtagtacc
    18841 gccttttacc ggacccgctt cacgggtcac gctacccccg ttttgacgga ttttttcgca
    18901 cgcttcagcg gcgttatcta cgctaagcgc gatgtgacca taagcagtgc cgagttcgta
    18961 tttatccacg ccccagttgt aggtcagttc aatcaccgct tcttcggttt ccgggccgta
    19021 gccaacaaac gccagtgagt atttgtattc cgggttttcg ctggtacgca gcagtttcat
    19081 gcccagcact ttggtataaa aatcgatgga gcgttgcaaa tcgccaacgc gcagcatggt
    19141 atgaagaaga cgcatctttt tatcctcaaa atggctaaca gattcaaaat gttgttttag
    19201 tatagcggcg gctttacgcc gctcgcaatg ttggattaca acgtcgggta atcggtatag
    19261 ccttccgcgc cgccaccgta gaaactttcg gcacgctgtg ggttaagctc agctttgcgc
    19321 tgcaagcggg cgaccagatc cgggttcgca atccagtcac gaccaaatgc caccgcatca
    19381 attaaccctt tgccgatcag cgtttcagct ttttctactg tgtatgcacc tgcgccgata
    19441 atcggaccgt ggaaacgggc gcgtactttt tcgcggaacg catcagtata cggttcaccc
    19501 cccgcccaat ctggttctga catatgcaga taagcaatgc cgcgtttacc cagttgttca
    19561 atcagataca gtgcatcggc ttcttcattc gggccgttat ccgtgttctg gaaagtaccg
    19621 attggcgaaa tgcgaatgcc aatgcgatcg gcaccccatt cttcaatccc ggcatcgacc
    19681 acttccagta ccagacgtgc gcgattttcc acgctgccgc cgtactgatc ggtacgatgg
    19741 tttgaagaag gagaaaggaa ctgatgcagc aaataaccgt gagcagagag gagctctacc
    19801 agatcaaaac cggcttcacg cgcgttagca atggcctgac ggaaatcatt gacgatacct
    19861 ggaatctctt ctagttcaag cgcacgcggc atggatgttt caacacggat cgcctgacca
    19921 ttttcatcgc gcagagaagt acgtgttccc gcgctaagtg ctgaaggcgc taccggtgcc
    19981 tgaccgccag gttgcaggct ggcgtgagaa atgcgtccgg tgtgccacag ctgcacggcc
    20041 atatgaccat tttcagcatg aacgccagcg gtgatttttt tccatgcggc aatttgctcc
    20101 ggactatgga tgccaggcgc acctgcatat ccttttgcct gggcagaaat ttgcgtggct
    20161 tcactaataa tcaaaccggc actggcacgt tggcgatagt attccgccat caacggggta
    20221 ggaatgtcac ccggttcaat actgcgcaga cgcgtcagcg gtgccataaa aatacggttt
    20281 gccgccgtga tcgcgcccac tttcagtggg gaatacagtt tttcagatga cataaaggct
    20341 cccggtcgtc tggtgataaa gaatgcctgc taaacggcag gcgtcgcaat aatgtttttt
    20401 acatgggcca gcgcgttttc cagtggctcg aaactgcgcg aaattttggc ctgcagattc
    20461 gcacccagcc atagtgcgta aagcacttgt gcctgttgta gcggttcgcc acaaaaggtt
    20521 aaacaatggt tctcacggcc attttccagc gcctgagaga gcagggcgat cacgccgcga
    20581 gcacctttat ccatcgcgct gcgcatatct tctgacagat cgcacacttc ggcagagagt
    20641 tttactgtca ggcaaccact gatggttcca tgttggcaaa actggttcag tgtttgctgg
    20701 taataagcca gtatgcggtc gcggtagtta ccttcgccgg attgcagcaa ctcagtcagt
    20761 cgctggtgat atgcggcgta atgacgctca agcatggcaa cgccaaacgc ttctttagag
    20821 cgaaagtagt gatagaagga ccctttcggc acttcagcgg tttttagtaa ttcgcttagc
    20881 cccatcccgg tgaatccacg ttgcaggcaa agttgctcgc ccgtcgccag gagatgttcg
    20941 cgagtatcat gttcggtgtg tttgttcatg cgttggagtg tagtagacca gtcggtctag
    21001 tgcaagcaaa gttgctactt tggacattaa cagcttcaat aatggtaata atcttatctg
    21061 aggagggcta agtggcggag caattagagt tctttcccgt ccagagcccg tgccggggaa
    21121 tttgccagtc tgacgaacgc gggttttgtc gcggctgttt tcgtagtcgg gatgagcgtt
    21181 ttaactggaa taaaatgagc gatggcgaaa aacaagaggt gctgcgcctt tgtcggcagc
    21241 gattaatgcg caaattacgc gcaaataagc cagcgtcatc agacgaaccg gagcaaccat
    21301 cactcttttg aacctaatct tgcgtatact cctctcatat ctgataagag gaagcgatta
    21361 tggttcagcg tattactatt gcgccgcaag gcccggagtt ttcccgtttt gtgatgggct
    21421 actggcgatt gatggactgg aatatgtccg cccgccagct ggtcagtttt attgaagagc
    21481 atctggatct cggcgtgacc accgtggacc atgctgatat ttatggtggc tatcagtgcg
    21541 aagcggcgtt tggcgaggca ctgaaactgg cacctcacct gcgtgaacgg atggaaatcg
    21601 tcagtaaatg cggtatcgcg acgaccgcgc gtgaagaaaa cgtcattggt cattacatca
    21661 ctgaccgcga tcacatcatt aagagcgccg aacagtcgct aattaatctc gcgaccgatc
    21721 atctggatct gctgttaatc caccgaccag acccgttaat ggatgccgat gaagtggcgg
    21781 acgcgttcaa acatctgcat cagagcggca aagtgcgtca ttttggcgta tcgaacttta
    21841 cgcctgcgca atttgccctg ttgcaatcac gtctgccgtt tacccttgcc actaatcagg
    21901 tggaaatatc cccggtgcat cagccgttac tgctggatgg cacgctcgac caactacaac
    21961 aactgcgtgt tcgtccgatg gcgtggtcct gccttggtgg tggtcgtctg tttaatgatg
    22021 attatttcca gccgctgcgt gatgaactgg ctgtggtggc agaggagtta aacgcgggct
    22081 cgattgaaca ggtggtttac gcctgggtat tacgtttacc atcgcagccg ctgccaatta
    22141 tcggttcagg taaaattgag cgcgtacggg cagctgtcga agcagaaaca ctgaaaatga
    22201 cccgtcaaca atggtttcgt atccgtaaag cggcactggg gtacgacgta ccgtaagcac
    22261 ttttaggaat agccgccgtt caaaaatgtg tcactggttt acacttattc aggaatgcac
    22321 aatgaacgga ggtcctatga aacgttttag tctggctatt ctggcgctgg ttgttgcaac
    22381 cggcgcacaa gctgccagtg aaaaagtcga gatgaacctc gtcacgtcgc aaggggtagg
    22441 gcagtcaatt ggtagcgtca ccattactga aaccgataaa ggtctggagt tttcgcccga
    22501 tctgaaagca ttaccccccg gtgaacatgg cttccatatt catgccaaag gaagctgcca
    22561 gccagccacc aaagatggca aagccagcgc cgcggaatcc gcaggcgggc atcttgatcc
    22621 acaaaatacc ggtaaacatg aagggccaga aggtgccggg catttaggcg atctgcctgc
    22681 actggtcgtc aataatgacg gcaaagctac cgatgccgtc atcacgcctc gtctgaaatc
    22741 actggatgaa atcaaagaca aagcgctgat ggtccacgtt ggcggcgata atatgtccga
    22801 tcaacctaaa ccgctgggcg gtggcggtga acgctatgcc tgtggtgtaa ttaagtaatt
    22861 aagaggcctg cgtaccttgc ggtggtgctt gctcaagttg cgaaagcgag cagtacagcc
    22921 gccagacaat tgccgccagc tcacgggcgg caggttgatg atgacgggca aggctgtcgc
    22981 aaatccgctg taattcttca agtgtggccg ccagtgattt ttgctgaacg ccacgctcac
    23041 tcatcactcc ccgcaacagt gaaatacagt tatcccgtac tcgcgataac ggatcggaac
    23101 gcgattccca gtcgcgcaat tgccagacaa catgagaaca gttcagcagc actacacccc
    23161 agcgtaataa ccaacggcga gccagcgcat cctgctgtta ctcaactgac tgacgtgatg
    23221 ataagtgagc gattcaaatt cgctttcact cagtgttgga tggcggctta gctgatcgac
    23281 aaaatcccgg cgcagcgcgc gaatatggcg gcggctttta cgagcatccg atcctggacg
    23341 cagaatggcg aacgctagcc aagccagcgc aacgccaacg atttttgcca gattatcgtt
    23401 aagaaaatca gcaaaatcat acaccggggg attagtgaca gcgataaacg aacccataaa
    23461 aacaatcagt tgcccccaca gtgcggcaaa tttgggcatc tgcaatttaa gaagctgcat
    23521 tgtcgccagc agtggaaaga gaaacagtaa aaattgccac agatcgctaa tctggaccat
    23581 caggccaaat ttgaccacaa agctgaatag cgaaagtaac accagcgtgc gcatcagcag
    23641 tgacaacgac ttaaacggtg ctgcgacggc ggagtagagt acgcagctaa ttgctgccag
    23701 cgttaatgca ttggcaccgg catcccattg cgatgcaata ctccatgcgc caatcatcat
    23761 caacgtacaa aatgtacgca gcccgctcca catagcttcg gcgttgtcgg tatgacgagc
    23821 cagagcggat gtccgtgcga gtctggcatg atcatctaca ccgctttgca gacgatgtaa
    23881 ttcctgacta ctttgcagat aaaggcggca aaaataacgt agtcgctgcc agaaggcgac
    23941 gtgccggtag tcggcgacgt tggtcgggcg tagcggggcg ataatacgtg cgacggtgta
    24001 agcatctgtt tgcgaactgg cgagcgccgt cagcaattgt tcgagaattt ctcgcgtggc
    24061 acctggcggt gagggccagt tgagcaacat acgtcgcagg ctggagatga tactggtcat
    24121 acgtaattgc tggtggagta gcgcattaag gcgcgcgttt tgctggcgaa aacgatagtg
    24181 gctccagaaa gcctggatac gcagcaaatt catggtcagt atctgcccaa tccccccctc
    24241 atgtgctgca cgaatggcat cgtttgtttc aggctgccag agtaaactgg catgttccag
    24301 taatcgggcg tgcatgtttt tcaatgcggt taaaagggct gtagcatcgg aactgctagg
    24361 caggatcatc atcatcatgc agccgcacaa aataccgact atcacctcgc aaacgcgcgc
    24421 ctgagcgata tcccacagct ggctggcctc agtaatatta accatcggaa aggcgatgat
    24481 ggcagccgtg tagcccgcca gttgaaatgc atacgcgacg ttattcgtga agtgcgcaca
    24541 ggcccaggta caaaagccaa gccacgccga catgctcaat agaaaaaacc acggctcatt
    24601 gagcgtatgc ccggcaagaa gcagtgccgc aatggctccc agtaaactgc cagcgatgcg
    24661 tccgaggctt ttgctgatga caccgccaac ggtgggaaag ctaaccactg cagccgaggt
    24721 catcgcccaa tagggttcat ccagattcaa ataataggca accgtcagcg ccagacacat
    24781 ggcaatggta ttgcgtaacg cataacgcca ttgcgccagc gtggccttga accagggcaa
    24841 attgcgcaag gaccatgacg atgcgttcat tattgaccta cggcaatgct acaggtggtg
    24901 cccgacacca gcgtgacatc gcctggaact ttatccagcg caaagcggac gggaacgcgt
    24961 tgggcgagac gaacccaggg gacgttaggt ttcacatccg gaataaggct tgagtcactt
    25021 tcaacgcttt gatcataaat cgcccgaccg atactggaaa cgtgaccccg taacgttttg
    25081 ttatcactat agagggtaat ttgtgctggt gcaccttcgc ggatatggcg caacttggtt
    25141 tcttcaaaat aaccaatgac gtaaaacgag tggctgtcga caagggcaaa cagcggtttc
    25201 ccggtgtcag cgtaatcgcc gatgcgggta gtgaggttag tcacccaacc actcaccggg
    25261 gcgcgaattt ccgtttgcgc cagttgccat tgtgtctgtt tcagcgtggc ctgtgcggca
    25321 ttaacactgg cctgcatcgc tttaacattg aggttagcgg tatcgagttc ttcggcagaa
    25381 ataaaatttt gtgagagatg acgacgacga ttcgcttcgt tattggcttt gcgaggtcag
    25441 attgcgcttt cgccagctga gcctgtgcgt tcagctcggc gatctgaaag ggcgttttat
    25501 cgatcgtgag cagaagatcc cccgcgttaa ccagttggtt gtcttttata ttcagctcaa
    25561 caatacgtcc cgacacctgt ggggtgatag aaaccttttc tgcgcgtatt ttcccgtcgc
    25621 gtgtccacgg agattgcatg taataattcc acagccacca tccggcaaga accgcaacta
    25681 tcgctacaat gattgttgag aaatacttaa ttgttttaat tgacatagtt accacgcaat
    25741 cagtattgcc agagcaaggc aaacgcaaat cgcaaacagc gataaatcca tttacaaggg
    25801 atgccagatg tcaccggcgt agatccagcc acgaagcagg cggtgtacga caagccagat
    25861 gacgaatcca aacgcgaagg ctttgaaaaa cggaggaaaa tagacggacg caccgaacac
    25921 cagatcttgt aagggcaatc ctgttgcatt gagcataaac ttcacaaagt agattccttt
    25981 acgaccgaat atattgcgtt agattaataa atattcttta agtgcgaaaa atttacgcgc
    26041 aatttctgaa aaatatgtct gacggtaacc aaatgcagca atacatttgt tttagcaata
    26101 aaattgctgc acactactct aaaagccgca taatatctta gcaagctaat tataaggaga
    26161 tgaaattgga atcgccacta ggttctgatc tggcacggtt ggtgcgcata tggcgtgctc
    26221 tgatagacca tcgcctgaaa ccgctggagt taacacaaac ccattgggtt acgttacaca
    26281 atatccatca gttacctcca gaccagtcgc aaattcaact ggcaaaagcg attggcatcg
    26341 agcagccatc actggtccgt actctggacc aactggaaga aaaagggtta atttcgcgtc
    26401 aaacttgtgc cagcgatcgt cgggctaaac gtattaaact gacggaaaag gcagagccac
    26461 tgatcagcga aatggaagct gttattaaca aaacccgcgc ggaaatatta catggcatct
    26521 ccgcagagga actggagcaa ctgattacgc tcatcgcaaa acttgagcat aatatcattg
    26581 agttacaggc taaagggtga aatgaagggg gcgtgtggtc agtaacctga ccacatgcaa
    26641 acttatttag cgcggagaaa cggtcacctg actgccattg ctggccagta cgacacgttg
    26701 gcccggagag aaacgagtgt tgccttgttt ctgtaccacc atgatggtat taccatcgtc
    26761 tttacgaatt tccagctcga caccctgcgt tttgttcatt gcactctgta cgccctgacc
    26821 agctacgcca cctgcaacag cgcctgctgc agtagccaga gaacgcccgg ttccgccacc
    26881 aacagtattc cccaggaaac caccaagaac agcaccgcca attgcaccga taacgttgga
    26941 atcatcaccg ccctgaatct gtaccggacg tacgttaacg atggtgccat agctgacatt
    27001 ctgtacttgt ttcgcttcag aagcggtata aacatcccct gacagggtgt cgttattaac
    27061 acaaccgaca agagacagac ctaccattga aacaaccaat acgcgtttaa tcattgaaaa
    27121 atctcctgtt caccatgaaa cgccacgcaa gcatccctca tggtcaaagt atatggcata
    27181 tgtgaatcca tggttacatg ttctaccaaa tatgagcaaa tcataatgaa agtaggatca
    27241 accaattata aacaaaacat tcatgatgat tattgatttg aagataatgt tttactacca
    27301 acggtaaaaa tcggtcattc cgcatggtat tcacgaaaag tttatgtttg agtggtgcgc
    27361 aaacgtcaga ttgaaaaagg atgaactatg aaatcgggcc gctttattgg cgttatgtca
    27421 ggcaccagcc ttgatggtgt tgatgttgtg ttggcgacaa tagatgaaca ccgggtcgca
    27481 cagctggcaa gcttgagctg gccgatcccg gtgtctctga aacaggctgt actggatatt
    27541 tgccagggcc agcagcttac actttcgcag tttggacagc ttgatactca actagggcga
    27601 ctttttgctg atgcggtcaa tgccttgctt aaggaacaaa acctgcaggc gagagatata
    27661 gttgcgatcg gctgtcacgg tcaaaccgtc tggcatgaac cgacgggcgt ggcaccacac
    27721 actttacaga ttggcgataa caatcaaatt gtggcacgca ccggaattac ggttgtcggt
    27781 gattttcgcc gtcgcgatat tgccttggga ggacaaggcg caccgctggt acctgcgttc
    27841 catcatgcct tgctggctca cccaaccgag cgacgaatgg tgctcaatat tggcggcatc
    27901 gccaatctgt cactgctcat tcctgggcag ccggttggcg gctacgatac cggtcctggt
    27961 aacatgctga tggatgcctg gatctggcgt caggccggta aaccttacga taaagatgcc
    28021 gagtgggcac gggcgggtaa agttattctc ccactgctgc aaaatatgct cagcgaccca
    28081 tatttctcgc aacctgcacc gaaaagcacc ggacgcgaat actttaacta cggctggctg
    28141 gagcgccatt tacgccattt tccgggtgtt gatccccgtg atgttcaggc cacactggca
    28201 gaactcaccg ccgtgaccat ttctgaacaa gttttgttga gcggtggctg cgaacgattg
    28261 atggtatgtg gtggaggtag tcgtaatccg ctactcatgg cgcgtctggc ggcattactg
    28321 ccaggcacag aagtcaccac caccgatgcc gttggcatta gtggcgatga catggaagca
    28381 ttggctttcg cctggcttgc ctggcggacg ctggcgggat taccaggaaa tctgccttcc
    28441 gtcactggcg caagccagga gacggtactg ggggctattt tccctgctaa cccgtgatat
    28501 cagagttaac tgaatttttc ttaccgcgtc tgccgttaca cttggaacgt tagggagggc
    28561 gtattgccct ccagaccagg aaagtcttcg ggaaatgtct atgaaaaaac tgttactgat
    28621 ctgtttgccg gtgcttctca ccggctgtag cgcgttcaat caactggttg agcacatgca
    28681 aaccgctacg ctggaatacc agtgtgatga aaaaccgttg acggtcaaac tgaataatcc
    28741 gcgccaggaa gtcagttttg tttacgataa tcaactactg catctcaaac agggcatttc
    28801 agcgtctggt gcacgttaca ctgacggaat ctatgttttc tggtcgaaag gcgatgaagc
    28861 gactgtctat aaacgcgacc gcatcgtatt gaataactgt cagttacaaa atccacagcg
    28921 ttgagatttt tccaggggcg gcgcacaata gcgtcaccca ctgacaatcc gtaaagaaaa
    28981 ccatgtctga taacgacgaa ttgcagcaaa tcgcgcatct gcgccgtgaa tacaccaaag
    29041 gcgggttacg ccgtcgcgat cttcccgccg atccattaag cctttttgaa cgttggctct
    29101 ctcaggcgtg tgaagccaaa ctggcagacc ctaccgcgat ggtggtcgct accgtggatg
    29161 aacatgctca gccttatcaa cgcatcgttt tactcaaaca ttacgacgaa aaaggcatgg
    29221 tgttttacac caacctcggt agccgtaaag cacatcaaat tgaaaataat ccgcgcgtta
    29281 gcctgctgtt cccgtggcat acccttgaac gccaggtgat ggtgatcggt aaagcagaac
    29341 gactttcgac tctcgaagtg atgaaatatt ttcatagccg cccgcgtgat agccagattg
    29401 gtgcatgggt ttcgaagcag tccagtcgca tttctgcccg cggtatcctt gaaagtaaat
    29461 tcctggagct gaagcagaag tttcaacagg gcgaagtgcc attgccgagc ttttggggcg
    29521 gttttcgcgt cagccttgaa cagattgagt tctggcaggg cggtgagcat cgcctgcatg
    29581 accgcttttt gtaccagcga gaaaatgatg tgtggaagat tgatcgtctt gcaccctgaa
    29641 aagatgcaaa aatcttgctt taatcgctgg tactcctgat tctggcactt tattctatgt
    29701 ctctttcgca tctggcgaaa agtcgtgtac cggcaaaggt gcagtcgtta tatacatgga
    29761 gattttgatg gcaagcagta acttgattaa acaattgcaa gagcgggggc tggtagccca
    29821 gatgacggac gaggaagcgt tagcagagcg actggcgcaa ggcccgatcg cgctctattg
    29881 cggcttcgat cctaccgctg acagcttgca tttggggcat cttgttccat tgttatgcct
    29941 gaaacgcttc cagcaggcgg gccacaagcc ggttgcgctg gtaggcggcg cgacgggtct
    30001 gattggcgac ccgagcttca aagctgccga gcgtaagctg aacaccgaag aaactgttca
    30061 ggagtgggtg gacaaaatcc gtaagcaggt tgccccgttc ctcgatttcg actgtggaga
    30121 aaactctgct atcgcggcga acaactatga ctggttcggc aatatgaatg tgctgacctt
    30181 cctgcgcgat attggcaaac acttctccgt taaccagatg atcaacaaag aagcggttaa
    30241 gcagcgtctc aaccgtgaag atcaggggat ttcgttcact gagttttcct acaacctgtt
    30301 gcagggttat gacttcgcct gtctgaacaa acagtacggt gtggtgctgc aaattggtgg
    30361 ttctgaccag tggggtaaca tcacttctgg tatcgacctg acccgtcgtc tgcatcagaa
    30421 tcaggtgttt ggcctgaccg ttccgctgat cactaaagca gatggcacca aatttggtaa
    30481 aactgaaggc ggcgcagtct ggttggatcc gaagaaaacc agcccgtaca aattctacca
    30541 gttctggatc aacactgcgg atgccgacgt ttaccgcttc ctgaagttct tcacctttat
    30601 gagcattgaa gagatcaacg ccctggaaga agaagataaa aacagcggta aagcaccgcg
    30661 cgcccagtat gtactggcgg agcaggtgac tcgtctggtt cacggtgaag aaggtttaca
    30721 ggcggcaaaa cgtattaccg aatgcctgtt cagcggttct ttgagtgcgc tgagtgaagc
    30781 ggacttcgaa cagctggcgc aggacggcgt accgatggtt gagatggaaa agggcgcaga
    30841 cctgatgcag gcgctggtcg attctgaact gcaaccttcc cgtggtcagg cgcgtaaaac
    30901 tatcgcctcc aatgccatca ccattaacgg tgaaaaacag tccgatcctg aatacttctt
    30961 taaagaagaa gatcgtctgt ttggtcgttt taccttactg cgtcgcggca aaaagaatta
    31021 ctgtctgatt tgctggaaat aatgcattaa gtggaaaggg ggagtgagaa atcactcccc
    31081 ctggttttta tacagggaac atgatgaaaa atattctcgc tatccagtct cacgttgttt
    31141 atggtcatgc gggtaacagt gcggcagagt ttcctatgcg ccgcctaggc gcgaacgtct
    31201 ggccgctgaa caccgttcaa ttttctaatc acacccagta cggcaaatgg actggctgcg
    31261 tgatgccgcc cagccattta accgaaattg tgcaaggcat tgccgccatt gataaattac
    31321 acacctgtga tgccgtgtta agtggctatc tgggatcggc ggagcagggt gaacatatcc
    31381 tcggtatcgt ccgtcaggtg aaagccgcga atccgcaggc gaaatatttt tgcgatccgg
    31441 taatgggtca tccggaaaaa ggctgtatcg ttgcaccggg tgtcgcagag tttcatgtgc
    31501 ggcacggttt gcctgccagc gatatcattg cgccgaatct ggttgagctg gaaatactct
    31561 gtgagcatgc ggtaaataac gtcgaagaag cggttctggc agcgcgcgaa ctcattgcgc
    31621 aagggccaca aattgtgttg gttaaacacc tggcgcgagc tggctacagc cgtgaccgtt
    31681 ttgaaatgct gctggtcacc gccgatgaag cctggcatat cagccgtcct ctggtggatt
    31741 ttggtatgcg ccagccggta ggtgttggtg atgtgacgag cggtttactg ctagtgaaac
    31801 tgcttcaggg ggcaacgctg caggaggcgc tggaacatgt gaccgctgca gtctacgaaa
    31861 tcatggtgac caccaaagca atgcaggaat atgagctgca agtggtggct gctcaggatc
    31921 gtattgccaa accagaacat tacttcagcg caacaaagct ctgaaatatc tcatttcggc
    31981 ccacaacgac ctgtgggccg atggctatta ctttaagcct tccgctgaca gcgcgtcttg
    32041 tacttccgga cgttcagcca tacgttgcat aaatgctgca atgtgctcta acccttccag
    32101 attcagtttc accgcgtatg cccagcgcag aaccgtaaac agataggcat cagcaattgt
    32161 aaatctttgc ccgcagatcc agtgctcatc cttcagtgcc tcgttcacat attgcagctt
    32221 cttctccagc tgcgcgcgaa ctgtcggttt gtactcttcc ggtgtatcag ggcgaaacag
    32281 aggtgtgaaa cctttatgca gctcggtggc gatgtaattc agccattcga tggttttata
    32341 gcgggaaata ctgtttaccg gtgccagcaa ctggcggtcg gggacgctgt cggcaagata
    32401 ctgcataatc gctacgcctt ccgtcagcaa agtatcgtca tccagcagca atgcaggcac
    32461 ctgcccctta gggttaacgg caaagtaatc gtcaccgttt tcgagacgtt ttttcattaa
    32521 atccacactg acgagggtaa aatcctttcc gctctcacgc agggtgatat gggaagcgag
    32581 agagcaggca cccggtttgt agaacaattt catcggtaac tccttttgtc tgtggtttaa
    32641 agtatggtag tgcgttgctg aacaaaaaaa aagccgctaa cgaaaagtta gcggcttgta
    32701 aaagagtttc cctgaatttt acgctacagc agctttcgcc gctttgtctg cagcgtcatc
    32761 ctgcgtcata cggtgcagtt taggcgcggt cagcagcatc agtactgcaa taatggcagt
    32821 agcgacacca atctgcaaga atacgcgacc ataaacttcc agtgacatca gcgggtcggt
    32881 aacgttatcc ggcacagcca tcatacccgc aacataacca ccaatcaggt ttgcaccggc
    32941 agtggtcagg aaccagctac ccataatgaa gcccatcaga cgctgcggaa cgagttgtgc
    33001 aaccattgcc agacccagac cagagatcat cagttccccg atgctctgca ggccatagct
    33061 tgcgaccagc cagcttacag acacgatacc agcgtcagac gcgaatttcg cacccagcgg
    33121 cagaatcagg aacgcaccag aacacatcac catgccgatt gcaaacttgg ttggcatcgg
    33181 cagggtatcg cccatcttgt tatagatagc ggccagaatc ggactaccga tgatgatcca
    33241 gaacgggttc agtgcctgat actgctcagg ttctacggcc agacccagaa tggagtgctc
    33301 aacgttacga atcgcaaaga agttcagtga cgttggcatc tggctgtaca gcacgaagaa
    33361 gataatggct tcgagcatca ggatgaaggc aacgatcatt ttacgacgcg cagcaccttt
    33421 catcgcgaag gcttctttac cgaagataac cacgataccg aaggcaacaa cgcccagcgc
    33481 catacgcgca acttcctgat tgtgcagcag ccaggtggcg atagcgatca gtgccacaac
    33541 accaataatg gtcagcagca ggttacggta gttgataggc tcgaagtctg gttttgaacc
    33601 gtactgttta acccagcgtt ggcagaaggc gaagttaacg atagtgatca gcaggcctac
    33661 aacgctcaac gcaaacgcaa cactccagcc gtatttcgcg gccagccacg gcgtagcaat
    33721 catagagaag aaagagccga tgttgacgga catgtagtac atggtgaatg caccgtccag
    33781 acgcgggtcg tttttctcat agcatgtaga aagcagagaa gacgggttag ctttaaacag
    33841 gccgttaccg accgcaatag ccgccatacc catataaacg ataccggcgt cgtgaccaga
    33901 ccaggcaacc agagcataac caatcgccag cacaatagcg ccgagcataa ttacgcgttt
    33961 agtacccagt accttgtcac ctaaccagcc gccgatagcg accagaccat aaaccagggc
    34021 actaaaggaa gagaaaaggg tgattgaatc cgcttcagac atacccagtt gtttaaccag
    34081 gtaaacagcc ataattcctt gtaggccgta ataaccaaaa cgttcccata actcaatcga
    34141 gaagatgaga tagaacgcct tcggttgttt gaaagcgttc aaactgacgc tttcagttgg
    34201 tttttggttt gcagtggaca cttttacctc tttttttaca tcccatatta acgggggtgc
    34261 tttctcgcgt tatgtctaaa aaacatacgc gcaattgtta tagtgggagg ggaaacggca
    34321 ggtaatgttc actatcttgc actttcagac aagtcctttg taataatctg ttacatataa
    34381 ctgaggcggg gaattcaacc attccgggaa atgttatcca gcgttaaatt tttcaaaaaa
    34441 cgtaacggca gattttttca ctgcactgag aggggggtga tggcttaacg gcgatatgtt
    34501 ctgctattta gcgtttttac tagtgagata gtccagtttc tgaaaaatag ccagtataat
    34561 gttttgtagg tcaatatttg ttgcgtgtgt aaatttaaat gattttaacc tgattcttgc
    34621 aatgttttta cattgcgaca gcatcgtgat cttgatcacg aatgaataga aaatttcgct
    34681 attaaaaaaa caattaacct gcatttttag ttatccgcaa acggattacc tgatgataac
    34741 gctaagcaat ggcatcattc acgttaaaaa gatgttttaa aagaggataa agaaaggtta
    34801 tcaatggggt aatcggtgtt accccttttc ttcagatgtc aactttctct ttgtattcac
    34861 aaagatcttc aataatacaa gagccacagc ggggcttgcg ggcaatgcag gtataacgcc
    34921 cgtgcaggat caaccaatgg tggcagtcga ctttaaactc tgccggaacc actttcagta
    34981 gcttttcttc tacctgttca acgtttttcc ccggcgcaaa ttgagtacga ttacaaacgc
    35041 ggaaaatgtg cgtgtcgaca gcaatagtcg gccagccgaa tgcagtgttt aatacgacgt
    35101 tggctgtttt acgacctacg ccgggcaggg cttcaagcgc agcacgatct tccggaacct
    35161 cgccattatg ctgctccagt aagatacggc aggttttgat gatattttct gctttgctgt
    35221 tataaagccc aatcgttttg atataggttt taaccccttc aacgcccagt tcaagcatcg
    35281 ctgcaggcgt attcgccacc gggtagagtt tcgccgtcgc cttattaaca ctgacatcgg
    35341 tcgcctgagc ggaaagcagt acggcaatca gcaattcaaa aggcgaactg aaattaagct
    35401 cggtggtggg atgaggattg ttctcacgca ggcgagtgag gatctccagg cgttttgctt
    35461 tgttcatcag acattccctg tttcaccgtt tggcaatgca cgttcggcag ctgcttcagt
    35521 acggcgtttt ttcattcttt catcaatcag gtattttcct gccagcatca gtcccaggcc
    35581 aataaatgca cctggtggca gcatcgccag caggaaaggg gagtcggtgt ggaaaatctc
    35641 cacgcgtaat acttttgccc agctacctaa cagcgcatct gcaccgtcaa acaatgtgcc
    35701 attgccgata atttcgcgta gtgaacccag cacgaacatg gcacaggttg cgcccatacc
    35761 aattgaaaag ccgtccagtg ccgaaagcgc cggacctttt ttggcggcga aggcttcagc
    35821 gcggcccaca acgatacagt tagtgacaat cagcggaata aaaatcccta atgattgata
    35881 caggccaaag gcgtaggcgt tgatcagcat ctgtacagcg ctgaccaccg aggcgatgat
    35941 catcacgtaa atgggaatgc ggatctcggc tggcgtccag tgacgcagcg tcgaaatggt
    36001 caggttggtc agcgtcagta ccagcgtagt cgcaagtcct aaacccagag cattagtggc
    36061 agtggacgtg accgccaaca gaggacaaag gccgagcaac tggaccagcg cagagttgtt
    36121 tttccacaac ccctgaacaa taacgtcttt aatttcgctc acgctttatt ctccacaggc
    36181 aggaagttga gaaagttgtg ccggtaacgt ctgagcgtac aatccggcgc gttttaccgc
    36241 attaaccacc gcgcggggag taatcgtcgc gccggtgaac tggtcgaaat caccaccatc
    36301 tttcttcacc gcccagtgcg catcatccgc accactgatt tttttacccg caaaatgggt
    36361 gatccagtca gaaaggcgca gttcgatttt atcgccaagc cctggcgttt cgtggtgctc
    36421 tgtcacgcgc gtgccaagta ccgtgccgtt aaaatcggct cccaccagca gctgaatcgc
    36481 accggaatag ccatctggcg cggttgcttc cagaacggcg gctaccggtt tgtcatcctg
    36541 tttggcgatg taaacccgat gctcaccttt acctaactct ggcgcagtta ccagatagca
    36601 actctgtgcc agcgcattgt tatagcgttc ggctggcagc acctgatcaa ataatgcctt
    36661 ttgttgcaga cttgcctgtt cagcaatcgt cgttttggtc atctggttga tggccgcagt
    36721 taaccctgtt gaacccgctg caaatagcgc taacgtaatg ccgtgttttc ggatagtttt
    36781 cagcatggtt taccctttgc gatggccgta gacgcgcgga cgcgtgtagt aatcgatcag
    36841 aggaaccgtg atgttcgcca gcaggacggc aaaagccacg ccgtcaggat agccgccgaa
    36901 actgcggatc aaccagacta ataagcccgc aagcgcgccg aaaataagac gaccacgatt
    36961 ggtcgtagaa gcggtaaccg ggtcagtcaa aataaagaat gcgccgagca tggtcgcacc
    37021 agacagcaga tgaatttgcg gtgctgccag tgtttctggt gagaacaacc agcccaacgt
    37081 tgcgcataac gccagcgtta ctaagaagct gaggggaata tgccagcgaa tcgctttctg
    37141 ccatagcaac cacaggccgc cagccagcca ggcgagattt acccattgcc aaccagcgcc
    37201 cgccagaata ccgctgtaga tcggatattg cataatctgt tcaaccgaat gaccggcacg
    37261 gacagaggtt ttaaatgtat ccagcggtgt cgcctgacta atgccatcaa tacctaagtg
    37321 tagtgtgttc atatcagcac cactggcggt atgcccgctg aaaataacct gtatggcgtc
    37381 gataaaacca gggatgttga ccgcaatttc atgtggcggt aaccagctgg tcatctgcac
    37441 cgggaaggag atcagtaaga ccacataacc aatcattgcc ggattaaacg ggttttgccc
    37501 cagaccgcca tacaactgtt tagcgatgat caccgcaaac accgaaccca gcacgaccat
    37561 ccaccatggc gcgagggggg gaatacttac cgccagcaat aagcctgtca gcaatgctga
    37621 gttatctttc aacgttgcgg ctaccgactg cttgcgtagt ttgagtacga gagcttcggc
    37681 taacagagta ctaaccgatg ccaacaggat ctgaacgaga gtaccccaac caaaaaacca
    37741 cagttgcgct gcgattcctg gcacggctgc gagcaacacc aacagcataa tgcgcgatgt
    37801 ctggcgctgg ttatgggtat aaggggagct agctattctg aataccattt agtcctcgtt
    37861 tacaaccttt tgctgggcgg cttttttggc ctgagcgcgg gcaatagccg cggcaactgc
    37921 cgctttgcgc ggatcaacct gttcttctgg ttctgcgtta gtctgttgct gttccagctt
    37981 gcgcgctttg gcacgggcaa tagcggcttc gacggcggct ttgcgcggat caacctgttc
    38041 ttcaggtacc gcattagcct gttgctgttc cagtttgcgc gctttggctc gggcaatagc
    38101 ggcttcgatg gcggctttgc gcggatcaac ctgttcttct ggttccgcat tagcctgttg
    38161 ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg ctttgcgcgg
    38221 atcgacctgt tcttctggtt ccgcattagc ctgttgctgt tccagcttgc gcgctttggc
    38281 gcgggcgata gctgcttcaa cggcagtttt acgtggatca gcaacggttg ctgcgtcgtt
    38341 agtttgctgc agttctgcct gtttcgctct ggcttgcgct ttacgggctt cccgtgctgc
    38401 aataattgca ctgttatccg ggcgttcgcc cgctttaatc acaataggct gtgtagcctg
    38461 ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc gcatctttat ctttggctgc
    38521 aggttgaacg gctgcgctct tatgtcgttc aaggcgagcc gctttttcgc gctccagacg
    38581 agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt cctgacgaat
    38641 agccgcaatt tcagcttttt cctgacggaa atattgcacc aggggaatat tgctcgggca
    38701 aacccacgcg caagccccac attcaatgca atcagcaatg ttatgcgtgg tagctttatc
    38761 gtgttgctga cctttgctga accagtacaa ctgttgcggc aaaagatccg cagggcaggc
    38821 gtcagcacag gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc caagttcatt
    38881 ggcagaggga gccaacagac agttggtaat ctttacgacc gggacatcca gccatggcaa
    38941 ggtaaagccc attagcgggc cacccataat caccatttga tcggcagagg ggcagaatcg
    39001 ggcatcattc aataaatgac gcactggcgt ccccagccgt gcccagacgt tgcccgggcg
    39061 agcgattgct tcgccagtca gggttacaac acgctcggta atcggctcgc catcaataac
    39121 ggcacgtttc actgcataag cagtgccgac gttttgcatt aatacgccga tatcggatga
    39181 acgcccgcca tgtggaacct gcttcccggt cagaatgtag gttaattgtt tagcaccgcc
    39241 agaaggatat ttggttggaa tcacccgcag agaaatatcg ttagagtccg ccagcaccgc
    39301 gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa tttcgcgtgg
    39361 ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc aatcctgcat
    39421 caaacggtca tcggcggtaa tgtacggctc gcactcagcc gcgttgataa tcaacgtttc
    39481 aatcttatct ccgccaccct gcaatttaac gcctgtcggg aatcctgcac cgcccagccc
    39541 ggcaacacca aactgatgta tgcgctcgat taactcttcg cgactgcgag tgcgataatc
    39601 ggcccagccg tcgcgcggga tccagcagtc ttcaccatcg gcatcaataa tcacgcttaa
    39661 ttcagctaaa gctgaaggat gagccgtaga gtggggcgca atagccgtaa cggtacccga
    39721 ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg gtaagcggct ggccgcgcaa
    39781 tactttatcg ccgacgctaa cgcacaactc accttcagcg ccaatatgct gtttcagtgg
    39841 aataacaaaa cgctgcgcca gtggtacctg gcgcaggggt gtaccgttgg actgggtttt
    39901 catctccggt ggatggatgc cgccgttgaa atcccagatt ttattttttc tgaatgcaga
    39961 gaataactta agcatggtgt tccacgggaa tgatacgcac gggaatggtg ttcagatccc
    40021 atttccagga gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc gtcgggcacg
    40081 gatcaacaca taaattgcag cccgtacaga gatcactcat taccgtatgc atggcacggg
    40141 tagcgccaac gatggcgtct accggacaag cctgaataca tttagtgcag ccaatacagt
    40201 tattttcatc aataaccgcc accatccgcg caggcgttag ctcttgcgct tcgccatcca
    40261 gcggctgcgg ctcgacatta agcaactcgg caatttttag catcacagct tcgccacctg
    40321 gggcgcaacg gttgattttt tcaccgttac agctgatggc ttccgcgtag gggcgacagc
    40381 cgggataacc gcactgacca cactggctct gcggtaagat ttcgtcaatt ttctcaacga
    40441 ccggatcgtc ttccaccgca aaacggcggg aggcataacc cagaatggcg ccaaacgcca
    40501 ggcccagcag gctcacggcg gcaacggcaa tccagatagc attcattaca acttcaccaa
    40561 accactaaag cccataaagg ccagagacat aagacctgcg gtaattaacg caatggcatt
    40621 accgcgaaaa ggtgccggga catcagccac agcaaggcgt tcgcggatgg cggcgaagag
    40681 caccatcacc agcgagaaac cgacagcggc ggaaaaaccg tacagcgccg actgcaagaa
    40741 attgtgcccg agattgatat tcagcaacgc cacgccgagc actgcacagt tggtggtgat
    40801 aagcggcaaa aaaatcccca gcagacggta aagcaccggg ctggttttgc gcaccaccat
    40861 ctcggtgaac tgcacgacca cagcaatcac cagaataaat gccagggtgc gcaggtaaat
    40921 cagattaagt gggatcaaaa tccacttatc gataagccag gcgcaaatag acgccagcgt
    40981 catcacaaac gttgttgcca gccccatgcc catcgcggtt tccagttttt tggaaacccc
    41041 cataaacgga cagagaccga gaaacttgac cagtacaaag ttattgacca gtacagttcc
    41101 gacaaacagt aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc cgcctattat
    41161 ccgttaatcc agagcaggcg acaacaggtt aactgtaagg ttattacgga ttcacaaacg
    41221 tcgcttttac ccgcgacgag cgtttgaaat aggagacgat caatgcggtt gccaacaaag
    41281 taaacagcaa ctggcgaacg gctatgccat cttccacggg tgaaaaagcg aatgctttta
    41341 ctgccagcaa tacggaaata agcagccaga taatatagtg tttaggaaca caacgccgac
    41401 gtttaaagaa agcgatggtc aaccacagtg tgtaatacca catggcgata gcggtaataa
    41461 aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc gccgagcgtt tgaaatgttt
    41521 gcggagaaga taacgcagcg gtatacagca acaacgccag cgtcgtactc aacagagcga
    41581 ctaaaagcca ggccaacggg ccaagcaacc agcctccaat acgttgtggc gttgtggtgg
    41641 tcataggttc tccagaatag cgacgcaaac aggcaaatct aattttaggg gcgttattat
    41701 aaggctttta taaccgatcg ctactttctt tattggacat agtgccagac ggacttcggt
    41761 acctggccga ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg gtgatcggca
    41821 gcacgataca tattgatcag ttcctgatcg tccgtcaggg tgtaattaag atggtcgtag
    41881 agtttttcca gactttcgag tgaactgatt ttgcgaaatt ttaataagta gtcctgaaca
    41941 gtcataaaag gtccataaaa taattaatac caaaggggta ggtattatta gtgatgataa
    42001 aatttcacca aaagtgaacg cttgtgttat tacaataata agaatgcgct gatggcaaat
    42061 attcagggat gaaaaactgc tctgaaaaga gtaacgcgct ttactatccc tgacaatcac
    42121 caacaacatc gaacaagata ataaattcct ggtttaatat ccgacaagtg aaaacatgca
    42181 cccggacggg cagcatggcg ctctacaagc gcagagctta cttgtgttgt accgaagcac
    42241 tctgttcagg tggctgatag ttgtcaatgt gactcgccac gccaagaaga atgactgaaa
    42301 cgacaagaac gatccaacct gttaattcaa taagacgatt cattacagcc cacatctctc
    42361 ttgattgatc cattaacttc agggggtaaa tgttacttag caataatagc tccagcaaga
    42421 tttttactga ggtttttgcg atatttagct tttgtcgttg gaaaattcgc tatttttttg
    42481 acttaagtta aacaacatcc cttattgctg gcaggttatt aaactgttga gcgtgggtaa
    42541 aggatagtgt caaatagcaa tcatacttca atgagaggca atgacatgag cgacaacatc
    42601 cgtgttgggt tgattgggta tggttatgcg agcaaaacct tccatgcgcc cctgattgcg
    42661 ggcacgcccg ggctggaact ggcggtaatc tccagcagtg atgaaacaaa agtaaaagcc
    42721 gactggccaa cggttacggt tgtctctgag ccgaagcatc tgtttaacga tcccaacata
    42781 gacctgattg tcattcctac acccaacgat acccatttcc cgttagccaa agcggcgctt
    42841 gaggcgggta aacatgtggt cgttgataaa ccctttaccg tgacactgtc acaagcgcga
    42901 gagctggatg cgctggcaaa aagcctgggg cgtgtgctgt ctgtattcca taaccgtcgt
    42961 tgggatagcg atttcttgac gctaaaaggt ttgctcgcgg aaggcgtgct gggtgaagtt
    43021 gcttactttg agtctcattt tgaccgcttc cgtccgcagg tgcgcgatcg ttggcgtgaa
    43081 cagggcggcc caggcagcgg tatctggtac gatttagcac cgcatcttct tgatcaggcc
    43141 attacgctat ttggtttacc ggtcagcatg acggtagatt tggcacagtt acggcccgga
    43201 gcgcagtcga ccgattattt ccacgccatc ttgtcctatc cacagcggcg agtcatttta
    43261 cacggtacca tgctggcagc cgctgagtca gcacggtata tcgtgcatgg atcccgaggc
    43321 agttatgtga aatatggcct cgatccacag gaagaacgtc tgaaaaatgg cgagcgtctg
    43381 ccgcaggaag actggggcta cgatatgcgt gatggcgtac ttacccgcgt ggaaggtgag
    43441 gaacgtgtcg aagaaacgct gttgacggta cctgggaatt atccggctta ctatgcggct
    43501 attcgtgatg cgttaaatgg cgatggtgaa aatccggttc cggcaagcca ggcaatccag
    43561 gtaatggagt tgattgagct gggcatcgaa tccgccaaac atcgcgcgac tttgtgcctt
    43621 gcatgaaaaa tggatggtgc aatttgcacc atccattttg ttacttcgcg gcgacttttt
    43681 ctcgcagtgc gcgtttttcc tcagcgctga ggaaagccat ttccagacca ttaatctgtg
    43741 cctggcggat ttgctcgcgg gataacccag cagctggcgc ggcaacggta tattcgtgaa
    43801 tgatatccac tccctgtacg ccgggatcgt cagtgttaat gccggcacga atgccatgct
    43861 caaggaacgt tttcagcgga tgtgcagcca gctctgctac ggtgctggtc tgaatattgg
    43921 aggtcagaca ggattcaata ccaatttgtt gctcggcgag aaaatccatc agcgcccgat
    43981 cttcaatggc ttttacgcca tgtccaatac gctccgcacc cagttcacga atcgcctgcc
    44041 agatgctttc cggcccggca gcttcgcctg catggacggt aatatgccag cccgcatcac
    44101 gcgcaaggtt gaagtgagaa aggaacagac ttcccgggaa accaagttca tcaccggcta
    44161 aatcaagtgc ggtaatctgg tcacggtggg ctaaaaaggc ctccagctct tgctgacagg
    44221 cggcttcgcc gaaggttcgg ctcataatgc cgataagctt cgcctgcaca ccaaaagtgc
    44281 ggcaaccttc acgtacgcca tcgatcaccg cttcgacaac acccgctaca ggcagcttat
    44341 gtgccattgc catgtagcct ggtgaaaaac gcagctcgac atagtgcagg ccgttacggg
    44401 ctgcatcttc aatgttttca aatgccacgc ggcggcaggc atcaagagag gcgagaactt
    44461 taacgcccca gtcaagtttg gtcagaaagc tcaccagatc gggttcgttg gcaatgacct
    44521 gaacgtgggg aatcagtgtt tccagggatt gtgcaggaag cgagatatta tactggcggc
    44581 caagttcaag aatggtctgg ggacgaatgt tgccatcaag gtggcgatgg atatcagtta
    44641 atggcagggt ggtatcaatc atggtcgcac tctttttctg gatataaaag tgcgcaccat
    44701 tataaaaaca aagcagggta aaaatccacg attgcgcaac gattttttac gttgcgcaat
    44761 tggggttaac gaacagcgcg gatggcgtta attaatccag ccacaccttt ttccagtttc
    44821 gaacgtgggc agccggcatt gagacggaca aaaccacgac cttcttcacc gtaggtatac
    44881 cccggcatga tcgcgactct ttcttgttcg ataagtgctt tttgcaacgc gttgtcgtca
    44941 atattcaacg gacgtaaatc aagccatgcc agataagtgg attgtgggat ctgccagttg
    45001 agttcaggaa acgcggcgtt cattttatct gcgatatacg tgaggttatc tttcagatag
    45061 atgcgtaagg catccagcca cggcgcgcct tgctgatagg cggcgatatg ggcagttaac
    45121 gccagtaccg aaggggaaga aagcccatca cggcctttca gtgccgataa ataggcatcg
    45181 cggctactgc tattttctat aatcccgtaa gcaccggtca gggcgggaat attgaaactt
    45241 ttcgagcccg acgttagcaa cgcccagtct ccgcgagcca cattactcca gggaatatgc
    45301 ggctgctcgc cccaaaccat atccatatgg atttcatcgg aaataacccg cacaccatga
    45361 cgctcgcaca ggtcagccat gatctccagc tcatcgcacg tccacacttt cccggtagga
    45421 ttctgtgggc tacacaggag cataatttta cattctggtt tcgccaacac ggcttccaac
    45481 ttgcccatat cgcaaaacca accatcagcc tgcttctcta aagcaacggg cattactgtg
    45541 cgctggttac cttcaatggc cttgtaaaat gcgtcatagg cgggtgtgtg gatcaccacg
    45601 ccttcacctg tttcagacca ctgacgaatc agttctgaaa ccatatagat gacagaaggg
    45661 ccatacacca ccgtctgaga atcgatggcg gtgtaatgct gggtggaaaa ccagtgggca
    45721 atagccgcga gaaactcatc gtttttccag cggctgtagc caaatacgcc gtgcatcagg
    45781 cgctgattca gcgcctctat aatgcagggg gcagtggcaa aatccatgtc tgaaatcgtg
    45841 aacggtaaca ggtcagcagt gccgaaacgg tcagcgacat aatcccactg tgtacaccat
    45901 gtgccatgac gatccacgac ctttgaaaaa tcgaacatat cttatcctta tgcctggaca
    45961 gtatgcatca gaccggccat ttcatctttt actgactgaa cttgtggccc gataacaacc
    46021 tgcaggttat gttgattaag ttgtactacg ccaattgccc gattgtcctt cagtgcctgc
    46081 acattaacaa gcgacatatc tttcacagac aaacgcagac gggtaatgca gttatcgagg
    46141 ctgacaatat tgtcggcacc gcctaatgct tcgaggattg caggaacgtt gtaacctgat
    46201 ttacccggcg cagcggcaac ggctttttcg attgagctgg caacttcgct atcgcgcccc
    46261 ggggttttga gattgaagcg ggtgatagcg aaacggaaga tggcgtagta aacgacaaac
    46321 cagattgccg ccaccactga caccatgtac cacttggttg acagaccatg caaaataccg
    46381 aacaccacga agtcgatgat attgccgtcg gtattaccga tggtgacgcc gagcacagac
    46441 atgacggtga agccgaggcc ggttaacagc gcgtggatga catacagaac tggcgctacg
    46501 aacaggaatt ccagcggttc ggtagtgccg ccaacgacgc aggcgatcag gccagaaatc
    46561 agcagacctt taattttatg gcgattttcc gggcgcgcgc agtgatacat agctaacgct
    46621 gcagctggca gaccgccgag aaacgcaggc attttacctt gcgaaaggaa acgcgtggcg
    46681 ctttcagaaa aaccgtgagt ggtcgggcaa ctcaattgcg cctggaagat ggtcagtgcg
    46741 ccgctgacgg tttgaccgca gacttcctgc gtgccgcctg cgtcggtaaa gcgaattaat
    46801 gccaccagaa tgtgatgcag accaaacggc aacagcagac gttcaccggt accaaacagc
    46861 atcggtccga aatcacccgc gctgtttatc atatggccca agccgctaat acccatggcg
    46921 aaaatcggcc agactaatgg aatcaccagg ccgacaaggc ccatcaccag cgaggagata
    46981 attgatacga agcgcgtacc gccgaagaat gccagcgcat ccggcaggcg gatattatgg
    47041 aaacgctcat gcagcatcca gacgataata ccggcgatca ccgcaccgag gatcccggta
    47101 tcgatcgact ggatcccaag aatgctctgg atgttattgg ctttcagaac cgcggcatcc
    47161 gtggttggca gaatgccttt attggtcaac cagaagttta ccgcgaggtt cattaccgca
    47221 taaccgacga agccagcgaa tgccgctacg cctttatttt cgcgtgccag gcccagcggg
    47281 atggcgatac agaacatgac aggcaggaaa ctaaaagcaa acgagccaat cttactcatc
    47341 caggtaaaga tagcctgcaa cacggggttg cccaggaccg ggatcagggt tatgacatca
    47401 tggctgctaa gagaactacc aatgccgagc ataatgccgc agaacgacaa taatgccacg
    47461 ggtaacatga aggttttgcc taactgctgg aagaactccc acagcgtgac tttcggtgct
    47521 gttttcgccg tcataaaacg actcctcgta gagaacaatc tggattcagt aaattgcgcg
    47581 agaagataaa acgttttatc aaattttagt gaggcataaa tcacattacg caacgataat
    47641 agcgggtata agataaataa aaggtaaaac gttttatctg tcacataatc agggagtagg
    47701 tcatctgcat ggctaccgcc aaaaaaataa ccattcatga tgttgcgctg gctgcgggcg
    47761 tgtcggtaag taccgtttcg ctggtgctta gtggcaaagg gcgaatctct accgccacag
    47821 gagaacgcgt taacgccgcc attgaagagc tgggatttgt gcgcaatcgc caggcgtcgg
    47881 cgctgcgcgg cgggcaaagc ggcgtcattg gtttgatcgt ccgtgattta tctgcgccgt
    47941 tttacgccga attgacggcc ggattgacgg aagctctgga agcgcaggga cggatggttt
    48001 ttttgcttca cggctgtaaa gacggcgagc agctggcaca gcggttttca ctgttactga
    48061 atcagggggt cgatggtgtg gtaattgccg gggctgcagg aagcagcgat gacctgcgac
    48121 ggatggcaga agaaaaagct atcccggtga ttttcgcttc ccgtgccagt tatcttgatg
    48181 atgttgatac ggttcgcccg gacaacatgc aggctgcaca gttgttgacg gagcatctca
    48241 ttcgcaatgg gcatcagcgg atcgcctggc tgggagggca aagttcctca ttaacccggg
    48301 cagaacgggt gggaggctat tgtgcaactc tactaaaatt tggcctgccg tttcacagcg
    48361 attgggtgct ggagtgcaca tccagccaga agcaagctgc ggaagctatc acggcgcttt
    48421 tacgtcataa cccgaccatc agtgctgtgg tttgctataa cgaaactatt gcgatggggg
    48481 catggtttgg tttgctgaaa gcagggcggc aaagcgggga aagcggagtc gatcgttact
    48541 ttgagcaaca agtttcgctg gcggcattta ccgatgcgac accaaccaca cttgatgata
    48601 tacccgttac ctgggccagc acgcctgcgc gggaacttgg taccacactt gcggatcgca
    48661 tgatgcaaaa aatcacccat gaagagacgc attcacgcaa tcttattatt cccgcccggc
    48721 tcattgcagc gaaataatcc tctctttatc tgctatactt ggtagtgtcc tttcctcaag
    48781 gttaatgata aaaaatgtgg cacacagg
//
LOCUS       DAJTNA010000009        43623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTNA010000009 DAJTNA010000000
VERSION     DAJTNA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43623
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.009"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..718
                     /gene="dgt"
                     /locus_tag="OO161_000432"
     CDS             <1..718
                     /gene="dgt"
                     /locus_tag="OO161_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCR5461615.1"
                     /translation="DDISYCVADLEDAVEKRICTVEQLYHHLHEAWGQHEKGSLFSLV
                     VENAWEKSRSNSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALL
                     EDASECSDLLKLYKNVAVKHVLSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTE
                     LVEKERVKRFPIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYI
                     SGMTDLYAWDEYRRLMAVEQ"
     gene            848..2272
                     /gene="degP"
                     /locus_tag="OO161_000433"
     CDS             848..2272
                     /gene="degP"
                     /locus_tag="OO161_000433"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5461616.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2427..3584
                     /gene="cdaR"
                     /locus_tag="OO161_000434"
     CDS             2427..3584
                     /gene="cdaR"
                     /locus_tag="OO161_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR5461617.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNCISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3673..4059)
                     /locus_tag="OO161_000435"
     CDS             complement(3673..4059)
                     /locus_tag="OO161_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5461618.1"
                     /translation="MYDNLKSLGITNPEEINRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4221..5033)
                     /gene="yaeI"
                     /locus_tag="OO161_000436"
     CDS             complement(4221..5033)
                     /gene="yaeI"
                     /locus_tag="OO161_000436"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR5461619.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5088..5912)
                     /gene="dapD"
                     /locus_tag="OO161_000437"
     CDS             complement(5088..5912)
                     /gene="dapD"
                     /locus_tag="OO161_000437"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5461620.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5943..8615)
                     /gene="glnD"
                     /locus_tag="OO161_000438"
     CDS             complement(5943..8615)
                     /gene="glnD"
                     /locus_tag="OO161_000438"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5461621.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGVQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8677..9471)
                     /gene="map"
                     /locus_tag="OO161_000439"
     CDS             complement(8677..9471)
                     /gene="map"
                     /locus_tag="OO161_000439"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5461622.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9839..10564
                     /gene="rpsB"
                     /locus_tag="OO161_000440"
     CDS             9839..10564
                     /gene="rpsB"
                     /locus_tag="OO161_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5461623.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10822..11673
                     /gene="tsf"
                     /locus_tag="OO161_000441"
     CDS             10822..11673
                     /gene="tsf"
                     /locus_tag="OO161_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5461624.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11820..12545
                     /gene="pyrH"
                     /locus_tag="OO161_000442"
     CDS             11820..12545
                     /gene="pyrH"
                     /locus_tag="OO161_000442"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5461625.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12837..13394
                     /gene="frr"
                     /locus_tag="OO161_000443"
     CDS             12837..13394
                     /gene="frr"
                     /locus_tag="OO161_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5461626.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13486..14682
                     /gene="ispC"
                     /locus_tag="OO161_000444"
     CDS             13486..14682
                     /gene="ispC"
                     /locus_tag="OO161_000444"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5461627.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDLCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14871..15629
                     /gene="ispU"
                     /locus_tag="OO161_000445"
                     /gene_synonym="uppS"
     CDS             14871..15629
                     /gene="ispU"
                     /locus_tag="OO161_000445"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5461628.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15642..16499
                     /gene="cdsA"
                     /locus_tag="OO161_000446"
     CDS             15642..16499
                     /gene="cdsA"
                     /locus_tag="OO161_000446"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5461629.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16511..17863
                     /gene="rseP"
                     /locus_tag="OO161_000447"
     CDS             16511..17863
                     /gene="rseP"
                     /locus_tag="OO161_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5461630.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17893..20325
                     /gene="bamA"
                     /locus_tag="OO161_000448"
     CDS             17893..20325
                     /gene="bamA"
                     /locus_tag="OO161_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5461631.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGVVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20447..20932
                     /gene="skp"
                     /locus_tag="OO161_000449"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20447..20932
                     /gene="skp"
                     /locus_tag="OO161_000449"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5461632.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20936..21961
                     /gene="lpxD"
                     /locus_tag="OO161_000450"
     CDS             20936..21961
                     /gene="lpxD"
                     /locus_tag="OO161_000450"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5461633.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22066..22521
                     /gene="fabZ"
                     /locus_tag="OO161_000451"
     CDS             22066..22521
                     /gene="fabZ"
                     /locus_tag="OO161_000451"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5461634.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22525..23313
                     /gene="lpxA"
                     /locus_tag="OO161_000452"
     CDS             22525..23313
                     /gene="lpxA"
                     /locus_tag="OO161_000452"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5461635.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            23313..24461
                     /gene="lpxB"
                     /locus_tag="OO161_000453"
     CDS             23313..24461
                     /gene="lpxB"
                     /locus_tag="OO161_000453"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5461636.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24458..25054
                     /gene="rnhB"
                     /locus_tag="OO161_000454"
     CDS             24458..25054
                     /gene="rnhB"
                     /locus_tag="OO161_000454"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5461637.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25091..28573
                     /gene="dnaE"
                     /locus_tag="OO161_000455"
     CDS             25091..28573
                     /gene="dnaE"
                     /locus_tag="OO161_000455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5461638.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMASKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVYPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28586..29545
                     /gene="accA"
                     /locus_tag="OO161_000456"
     CDS             28586..29545
                     /gene="accA"
                     /locus_tag="OO161_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5461639.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVHLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            29644..31785
                     /gene="ldcC"
                     /locus_tag="OO161_000457"
     CDS             29644..31785
                     /gene="ldcC"
                     /locus_tag="OO161_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5461640.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPVHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     HREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLWAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFEADIHGAKQDEDGVYRVRVLKMAG"
     gene            31842..32231
                     /locus_tag="OO161_000458"
     CDS             31842..32231
                     /locus_tag="OO161_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5461641.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            32296..33594
                     /gene="tilS"
                     /locus_tag="OO161_000459"
     CDS             32296..33594
                     /gene="tilS"
                     /locus_tag="OO161_000459"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5461642.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIMPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(33643..33903)
                     /gene="rof"
                     /locus_tag="OO161_000460"
     CDS             complement(33643..33903)
                     /gene="rof"
                     /locus_tag="OO161_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5461643.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(33890..34090)
                     /locus_tag="OO161_000461"
     CDS             complement(33890..34090)
                     /locus_tag="OO161_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5461644.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            34256..34801
                     /locus_tag="OO161_000462"
     CDS             34256..34801
                     /locus_tag="OO161_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5461645.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAVQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            34798..35160
                     /gene="arfB"
                     /locus_tag="OO161_000463"
     CDS             34798..35160
                     /gene="arfB"
                     /locus_tag="OO161_000463"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5461646.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLA"
     gene            35234..35944
                     /gene="nlpE"
                     /locus_tag="OO161_000464"
                     /gene_synonym="cutF"
     CDS             35234..35944
                     /gene="nlpE"
                     /locus_tag="OO161_000464"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5461647.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(36144..36968)
                     /locus_tag="OO161_000465"
     CDS             complement(36144..36968)
                     /locus_tag="OO161_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5461648.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHIAIFLGDNN
                     VAEATGAGVHIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(37021..38739)
                     /gene="proS"
                     /locus_tag="OO161_000466"
     CDS             complement(37021..38739)
                     /gene="proS"
                     /locus_tag="OO161_000466"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5461649.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(38850..39557)
                     /gene="tsaA"
                     /locus_tag="OO161_000467"
     CDS             complement(38850..39557)
                     /gene="tsaA"
                     /locus_tag="OO161_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5461650.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(39554..39958)
                     /gene="rcsF"
                     /locus_tag="OO161_000468"
     CDS             complement(39554..39958)
                     /gene="rcsF"
                     /locus_tag="OO161_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5461651.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(40076..40891)
                     /gene="metQ"
                     /locus_tag="OO161_000469"
     CDS             complement(40076..40891)
                     /gene="metQ"
                     /locus_tag="OO161_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5461652.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(40931..41584)
                     /gene="metI"
                     /locus_tag="OO161_000470"
     CDS             complement(40931..41584)
                     /gene="metI"
                     /locus_tag="OO161_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5461653.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(41577..42608)
                     /gene="metN"
                     /locus_tag="OO161_000471"
     CDS             complement(41577..42608)
                     /gene="metN"
                     /locus_tag="OO161_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5461654.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            42796..43368
                     /gene="gmhB"
                     /locus_tag="OO161_000472"
     CDS             42796..43368
                     /gene="gmhB"
                     /locus_tag="OO161_000472"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5461655.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        10595 a        10686 c        11874 g        10468 t
ORIGIN      
        1 cgacgacatc tcctattgtg tggcagacct tgaagatgcg gtagagaaaa gaatatgtac
       61 cgttgagcag ctttatcatc atttgcacga agcgtggggc cagcatgaga aaggttcgct
      121 cttttcgctg gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt taagccgcag
      181 tacggaagat cagtttttta tgtatttacg ggtaaacacc ctaaataaac tggtacccta
      241 tgcggcacaa cgatttattg ataatctgcc tgcgattttc gccggaacgt ttaatcatgc
      301 attattggaa gatgccagcg aatgcagcga tcttcttaag ctatataaaa atgtcgctgt
      361 aaaacatgtg cttagccatc cagatgtcga gcagcttgaa ttgcagggct atcgggtcat
      421 tagcggatta ttagagattt atcgtccttt attaagcctg tcgttatccg actttactga
      481 actggtagaa aaagaacggg tgaaacgttt ccctattgaa tcgcgcttat tccacaaact
      541 ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt aaattaccgt cagattctcc
      601 tgagtttccg ctatgggaat attattaccg ttgccgcctg ctgcaggatt atatcagcgg
      661 tatgaccgac ctctatgcgt gggatgaata ccgacgtctg atggccgtag aacaataacc
      721 aggcttttgt aaagacgaac aataaatttt taccttttgc agaaacttta gttcggaact
      781 tcgcgctata aaacgaatct gaagaacaca gcaattttgc gttatctgtt aatcgagact
      841 gaaatacatg aaaaaaacca cattagcact gagtgcactg gctctgagtt taggtttggc
      901 gttatctccg ctctctgcaa cggcggctga gacttcttca gcaacgacag cccagcagat
      961 gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca ttaacgtaga
     1021 aggtagcaca accgttaata cgccgcgtat gccgcgtaat ttccagcagt tcttcggtga
     1081 tgattctccg ttctgccagg aaggttctcc gttccagagc tctccgttct gccagggtgg
     1141 ccagggcggt aatggtggcg gccagcaaca gaaattcatg gcgctgggtt ccggcgtcat
     1201 cattgatgcc gataaaggct atgtcgtcac caacaaccac gttgttgata acgcgacggt
     1261 gattaaagtc cagctgagcg atggccgtaa gttcgacgcg aagatggtcg gcaaagatcc
     1321 gcgctctgat atcgcgctga tccaaatcca gaacccgaaa aacctgaccg caattaagat
     1381 ggcggattct gatgcactgc gcgtgggtga ttacaccgta gcgattggta acccgtttgg
     1441 tctgggcgag acggtaactt ccgggattgt ctctgcgctg gggcgtagcg gcctgaatgc
     1501 tgaaaactac gaaaacttca tccagaccga tgcagcgatc aaccgtggta actccggtgg
     1561 tgcgctggtt aacctgaacg gcgaactgat cggtatcaac accgcgatcc tcgcaccgga
     1621 cggcggcaac atcggtatcg gttttgcaat cccgagcaac atggtgaaaa acctgacctc
     1681 gcagatggtg gaatacggcc aggtgaaacg cggtgagctg ggtattatgg ggactgagct
     1741 gaactccgaa ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt tcgtaagcca
     1801 ggttctgcct aattcctccg ctgcaaaagc gggcattaaa gcgggtgatg tgatcacctc
     1861 actgaacggt aagccgatca gcagctttgc cgcactgcgt gctcaggtgg gtactatgcc
     1921 ggtgggcagc aaactgaccc tgggcttact gcgcgacggt aagcaggtta acgtgaacct
     1981 agaactgcag cagagcagcc agaatcaggt tgattccagc tccatcttca acggcattga
     2041 aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca acgtgaaaac
     2101 gggcactccg gctgcgcaga tcggcctgaa gaaaggtgat gtgattattg gcgcgaacca
     2161 gcaggcagtg aaaaacatcg ctgaactgcg taaagttctc gacagcaaac cgtctgtgct
     2221 ggcactgaac attcagcgcg gcgacagcac catctacctg ttaatgcagt aatctcactc
     2281 aaccccttcc tgaaaacggg aaggggttct ccttacaatc tgtgaacttc accacaactc
     2341 catacatctt catcatcctt taggcatttg cacaatgccg tacgttacgt acttccttat
     2401 gctaagccgt gcataacgga ggacttatgg ctggctggca tcttgatacc aaaatggcgc
     2461 aggatatcgt ggcacgtacc atgcgcatca tcgataccaa tatcaacgta atggatgccc
     2521 gtgggcgaat tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac gaaggtgcat
     2581 tgctggtact ttcacaggga cgagtcgtcg atatcgatga cgcggtggca cgtcatctgc
     2641 acggtgtgcg gcaagggatt aatctaccgt tacggctgga aggtgaaatt gtcggcgtaa
     2701 ttggcctgac aggtgaacca gagaatctgc gtaaatatgg cgaactggtc tgcatgacgg
     2761 ctgaaatgat gctggaacag tcgcggttga tgcacttgtt ggcgcaggat agccgtttgc
     2821 gggaggaact ggtgatgaac ctgattcagg cagaggagaa tactcccgca cttactgaat
     2881 gggcgcaacg gctggggatc gatctcaatc aaccgcgagt ggtggctatt gttgaggtcg
     2941 acagcggtca gcttggtgtg gacagcgcaa tggcggagtt acaacaactg caaaacgcgc
     3001 tgactacgcc cgagcgtaat aatctggtgg cgattgtctc gctaaccgaa atggtggtgt
     3061 tgaaaccggc gttgaactct tttgggcgct gggatgcaga agatcatcgt aagcgagttg
     3121 aacaactgat tacccgcatg aaagagtacg gccagctgcg ttttcgcgtt tcactgggca
     3181 actattttac cggtcctggc agtattgccc gatcctatcg tacggcgaaa acgacgatgg
     3241 tggtgggtaa acagcggatg ccagaaagtc gctgctattt ttatcaggat ctgatgttac
     3301 ctgtgttact cgacagtttg cgtggcgact ggcaggccaa cgaactggcg cgaccgctgg
     3361 cgcggctgaa agcgatggac aataacggct tgctgcgacg aacgctggcg gcgtggtttc
     3421 gtcacaatgt gcaaccgctg gcaacgtcaa aggcgttgtt tattcatcgt aataccctgg
     3481 agtatcggct taattgtata tcggaactga ccgggcttga tttgggcaat tttgatgaca
     3541 ggttgctgct gtatgtggcg ttacaactgg atgaagagcg gtaggttatg cgttaaggtt
     3601 gaggcggcgc tgtctcattc ccctcagccc gaaggggaga ggggaatgcc cgaattaacc
     3661 gtcaattcgg gattatttac gcgttagttt ttccagatcg gcttcaatct cgctgatctt
     3721 attggttacg actgactcca ggtgacgtaa gtcgtcgagg atcttacgct taagatcaac
     3781 ttcgctgcgg tcacgctggc agatttgatc aagctcatca atgatatacc gtagattcgg
     3841 gctgatttcc tggacttctt tataaccctg acccacacca tcagcgacga ccgttttacg
     3901 ctgacgcgga tatttaaact taacgctctt ggcgaaaaac tcgcctttgt ctttctggaa
     3961 atagattttc agaatatcgt tgttggcttc ctgccggagg ctgtaacgat taatttcttc
     4021 aggattggta atacccagac ttttcagatt gtcgtacata gcgttacctc aaaatgagtc
     4081 agtaaattgt gcttatttta gcatttggcc tggcccgccc ggctcgactg tgatcgcaga
     4141 aagccttttt cttccagact tctatcatgg cgtaaaaatc aaaaaattac ctgctttatt
     4201 gtggtgataa aattcacgtt ctacaccagt tccagcatcg ttacttccgg gcggcagttc
     4261 agacgcaaac catacaaact gcccacgcca cgggttgtgt agatgtgtcg ttcgccaaag
     4321 gcatttaatc cggcgacgta acgtttatct tcgacagggg caaaaggttc accaaccaac
     4381 ggtacgcgca gttgcccgcc gtgagtatgg ccgcacagca tcagatccca gggttcatca
     4441 cgcatgactt ctttgctgtc gggattatgt gccagcatca gtcttggcag atttgcttcg
     4501 ctggcgggag gtggtttgca ttgtccggcc cataagtcac cggtgcctac cagttcgaat
     4561 tgcctgttcg gcgtggcgat caccgtagcc tggttaaaca acaccgtgat gcccactgat
     4621 ttcaacgtct cgccaattaa gtgatttttt tctgtaccaa cagggcgatc gtggttgccg
     4681 aagcaggcaa acgtcggcgc acattcggca aggggggaga ggacatcact aaacgccgaa
     4741 aaattcagcg gcatatcaaa taatacgtaa tcgccgccca gtaatatcaa atcgggcttt
     4801 tgttctatgc caagagcaat cgcgtcagaa atcaggctta aaggaacaaa acgagagtaa
     4861 tggagatcgg ccagaaaaag aattttgaat ggtgctgcgt tgtctttaaa aaaggcgagg
     4921 cggtgacgga ttaattcaaa ccagcctggc tcccagtaat gcatataacc gaaacctgag
     4981 ctcgtggcta tcgtcgcggc agtagcctgc aacaatcggc ggcgtgaaat catcgctcat
     5041 ccctgcaaaa aaaatcgggc agcgtcgtgc tgcccgtgtg catactttta gtcgatggta
     5101 cgcagcagtt cgttaatgcc gactttgccg cgagttttcg cgtcaacttt cttaacgata
     5161 accgcacagt agaggctgta tttgccatct tttgacggca gattacctga aacaaccaca
     5221 gaccccgccg gaacgcgacc gtagtggatt tcgccggttt cacggtcgta aatacgggtg
     5281 ctctgaccaa tgtatacgcc catggaaatg acggaacctt cttcgacaat caccccttca
     5341 actacttcag agcgtgcgcc aatgaagcag ttatcttcaa tgatggttgg gttagcctgc
     5401 agcggttcca gcacgccgcc gatgcccacg ccaccggaaa ggtggacgtt tttaccaatc
     5461 tgcgcacaag aaccgacggt cgcccaggta tcaaccatgg tgccttcatc aacatatgcg
     5521 ccgatgttga cgtaagacgg catcagcacg gtgttacggg caataaacgc accctgacgt
     5581 accgccgctg gtggcacaac gcggaagcct tctttctgga aacgtgcttc gtcgtagtcg
     5641 gcgaatttca tcggcacttt gtcgaagtag cggctttctg ccccttcgat cacctgatta
     5701 tcattaatac ggaaagagag cagcaccgct tttttcaacc actgatgcgt cacccactga
     5761 ccgtcaattt tttccgctac acgcagtgca ccggaatcca gcagggcgat cacctgattt
     5821 accgcttcgc gggtaacggt gtctgcgttg gctggcgtga tctcggcacg gcgttcaaaa
     5881 gcggtttcaa taatgttctg taactgctgc attgttaaac tcttttcata tcagtaaaca
     5941 catcaccctt tatcgtttgg attgagggcc tctgtcaacc gctgatgcac ttcctgctgc
     6001 aactcgttat taagcgcacg ccggtcagcg gtggcaatta tgaataaatc ttctactcgc
     6061 tcgccaatgg ttgtaattcg ggcaccatga agcgaaattc ccagatcggc aaaaattttc
     6121 ccgactctcg ccagcagtcc tggctggtcg agcgcgataa gctcaaggaa cgatttgcgg
     6181 tcggtatggg tcggcaaaaa cgttacttcg gtttcaacag taaaatggcg taatttggcg
     6241 ggttggcgac ggggctgcgg tggctgccag ctactttgcg tcagtacttg ctccagacca
     6301 aaccgaataa cctcatgacg atctgcggac agcgggctgc catcgggttc cagcacgata
     6361 aaggtatcca tcgccatacc gtcgcgagtg gtgaaaattt gtgcgtcgtg aacacttaaa
     6421 ttgcggcggt ctaattcggc acagacggcg gcaaacagat aggggcggtc cgggctccag
     6481 ataaaaatct cggtgcctcc acgcgtagcc tgcgggctaa gcaataccag tggtttgctt
     6541 aaatcatgct gtaataaatg gcgggcatgc caggccagtt gatttgggct atggcggaca
     6601 aaatagttag cacgacagcg tgaccaaatt tggtgcagcg cctcttcgtc gatgttatcc
     6661 atgcgcagta gtgccagtgc ctggagttgg tgatggcgaa cccgttcgcg catatccggc
     6721 gtgttttgca ttccgcgtcg tagctgcttt tcggtggcaa aatagagttc gcgcaacaga
     6781 ctttgcttcc agctattcca cagcgtttcg ttggtggcgc aaatgtcagc cactgtcagg
     6841 cataccagat agtgcagacg attttccgtt tgaacttctt cggcaaactg cttgatgact
     6901 tcagggtcct gaatatcgcg gcgttgtgct gtcaccgaca tcagcaagtg ctggcgaacc
     6961 agccaggcaa ccagctgtgt ttcgcgtgag ttcagcccgt ggagttctgc aaaatgcact
     7021 acatcctgaa caccgagaat ggagtggtcg ccgccgcgtc ctttggcgat atcgtgaaac
     7081 agcgcggcga tgaaaatcag ctcagttgac ggcaggcgcg gccagacgtc cacacacaac
     7141 ggatggcgct ggcgcgtttc ttcactggca aaactctcca gtttcagcat cacgcggata
     7201 gtatgttcat ccaccgtgta ggcgtggaac agatcaaact gcatctgccc gacgatatgc
     7261 gaccattgcg gcatatacgc gccgagcacg ctatggcgat gcattggcaa tagcccgcgc
     7321 cgcaccgctc cggggtgacg cagaatgctc aaaaacagtt ttcgtgcttc cggaatatta
     7381 cacagcggtt gttgcagatg gcgacgggca tggcgtaact ggcgcagcgt ggtggagtaa
     7441 atgccggtga tcgcactgtt gcgcaccatg gtgtagaaca tacgcaagat ggcttccggc
     7501 tggcgcataa atagtgtttc atcacgcagg tcgattagcg taccgcgtag ctgaaactca
     7561 tcgtcgattg gacgtggttt ttcgtcggca ggaagggcga ggatggcttc atcgaacagt
     7621 tgcagcagca tctggttgag ttcactgacg cggcgtgtaa cgcggaagta atccttcatc
     7681 atccgctcga ccggttcgtt gccttcacca ctgtaattca gacgctgagc gacgctaagt
     7741 tggcgatcga ataacaggcg attatcgtaa cggctgacga ccagatgcag ggcaaagcga
     7801 atacgccaca gtatatgcag acattcgttt aattccgccc gctccgctga ggttaagaag
     7861 ccaaacccaa ccatttcatc cagcgatgtt gcgccaaaat gacggcgggc cacccattgc
     7921 agagtgtgga tatcgcgcaa gccgccaggg ctacttttga tgtctggttc aagattgtag
     7981 ctggtgccgt ggtaacgctg atggcgctgg ttctgttctt caactttcgc cgcgtagaac
     8041 ttgtcggaag gccagaatcc ttcgctaaaa atatgttttt gcagttcgag gaagagcgca
     8101 acatcgccaa ttaataagcg ggattcgatt aaattggtgg cgacggttaa atccgataac
     8161 ccttccagca tgcactcttc aagcgtgcgc acactatgac cgacttccag ctttacatcc
     8221 cagagcagcg ttaacagctc gcccactttt tgcgcctgat cgtccgggag ctttttacgg
     8281 cttaaaatca gtaaatcgat gtcagaaagt ggatgcagct cgccacgacc gtagccaccg
     8341 acggcgacca atgccaggtc ggcaatctgg ctgaatcccg cttcaatcca taatcgttgc
     8401 aggagctggt cgataaactc ggtgcgcgcc tcaatcaact gttctgcaga gatcccattg
     8461 tcaaaggcat cacccagcca acgctggaaa gtatcgatat gggcttttat cccaccgacg
     8521 gttaattcat cacggggcca gacgcatgga ttttgcggtt gaccgggcag ggtggggaga
     8581 gcggtgtttg cgtactgttc tggaagggta ttcattgtgc gccactcata agattaaatt
     8641 atcgcattaa aaaagccggc attcgccggc ttcatcttat tcgtcgtgcg agattatcgc
     8701 cgggatggtg tcatccttgc gtagcgtcag aatttcgcag ccgttatcag tcaccacaat
     8761 agtatgctca tattgtgcag acaagctgcg atctttggtt tttaccgtcc agccatcttt
     8821 catggtgcgg atctcttttt tacccgcgtt gaccattggc tcgatggtga acgtcatccc
     8881 aggtttcagt acgacgttgg tttcacggga gtcatagtgc agcacctgcg gttcttcatg
     8941 gaagccgcga ccaataccgt gtccgcaata ttcacgaacg acggagaagc cttctgcttc
     9001 gacaaacttc tgaatcgccg caccgatttc gcgcaggtta atgcctggtt tgaccatgcg
     9061 cagcgccagg tacaggcttt cttgcgtgat gcggcacaga cgttcgccca tgatggtcgg
     9121 cttaccgacg ataaacattt tcgaggtatc gccgtggaaa ccatctttga ttacggtgac
     9181 atcaatgtta acgatatcgc catctttcag cagcttcgca tcgtccggga taccgtggca
     9241 caccacttca ttaatagaga tgcaaacgga tttcggatag ccgtgatagc cgaggcaggc
     9301 agaaaccgcg tgttgttcat taacaatgta atcattacag atgcgatcca gctcgccggt
     9361 gctgacgccc ggtttaacat acggttcgat catctccagc acttcggcag ccagtcggcc
     9421 agcgacgcgc attttttcga tatcttctgg ggtcttgatt gagatagcca ttaattctgt
     9481 ccatcagcgt cggtgatacc gacaatatat atgtaagtgc cgtcaatggt atcacacccg
     9541 ggcaaattga gaatcattct gaatttcgcc aaacgtgcca ctgaacgttt tctataatag
     9601 aaaattcaac gtctgatgct gtacacagcg ccaacaatta ttggtgtcca tgacgtattt
     9661 gtggtataaa gcgcgccgga cttccgatcc atttcgtata cacagactgg acggaagcga
     9721 caatctcact ttgtgtaaca acacacacgt atcggcacat attccggggt gccctttggg
     9781 gtcggtaata tgggatacgt ggaggcataa ccccaacttt tatatagagg ttttaatcat
     9841 ggcaactgtt tccatgcgcg atatgctcaa ggctggtgtt cacttcggtc accagacccg
     9901 ttactggaac ccgaaaatga agccgttcat cttcggtgcg cgtaacaaag ttcacatcat
     9961 caaccttgag aaaactgtac cgatgttcaa cgaagctctg gctgaactga acaagattgc
    10021 ttctcgcaaa ggtaaaatcc ttttcgttgg tactaaacgc gctgcaagcg aagcggtgaa
    10081 agacgctgct ctgagctgcg accagttctt cgtgaaccat cgctggctgg gcggtatgct
    10141 gactaactgg aaaaccgttc gtcagtccat caaacgtctg aaagacctgg aaactcagtc
    10201 tcaggacggt actttcgaaa agctgaccaa gaaagaagcg ctgatgcgca ctcgtgagct
    10261 ggagaaactg gaaaacagcc tgggcggtat caaagacatg ggcggtctgc cggacgctct
    10321 gtttgtaatc gatgctgacc acgaacacat tgctatcaaa gaagcaaaca acctgggtat
    10381 tccggtattt gctatcgttg ataccaactc tgatccggac ggtgttgact tcgttatccc
    10441 gggtaacgac gacgcaatcc gtgctgtgac cctgtacctg ggcgctgttg ctgcaaccgt
    10501 acgtgaaggc cgttctcagg atctggcttc ccaggcggaa gaaagcttcg tagaagctga
    10561 gtaataaggc ttgataactc ccccaaaata gttcgagttg cagaaaggcg gcaagctcga
    10621 gaattcccgg gagcttacat cagtaagtga ccgggatgag cgagcgaaga taacgcatct
    10681 gcggcgcgaa atatgaaggg ggagagccct tatagaccag gtagtacacg tttggttagg
    10741 gggcctgcat atggccccct ttttcacttt tatatctgtg cggtttaatg ccgggcagat
    10801 cacatctccg aggattttag aatggctgaa attaccgcat ccctggtaaa agagctgcgt
    10861 gagcgtactg gcgcaggcat gatggattgc aaaaaagcac tgactgaagc taacggcgac
    10921 atcgagctgg caatcgaaaa catgcgtaag tccggtgcta ttaaagcagc gaaaaaagca
    10981 ggcaacgttg ctgctgacgg cgtgatcaaa accaaaatcg acggcaacta cggcatcatt
    11041 ctggaagtta actgccagac tgacttcgtt gcaaaagacg ctggtttcca ggcgttcgca
    11101 gacaaagttc tggacgcagc tgttgctggc aaaatcactg acgttgaagt tctgaaagca
    11161 cagttcgaag aagaacgtgt tgcgctggta gcgaaaattg gtgaaaacat caacattcgc
    11221 cgcgttgctg cgctggaagg cgacgttctg ggttcttatc agcacggtgc gcgtatcggc
    11281 gttctggttg ctgctaaagg cgctgacgaa gagctggtta aacacatcgc tatgcacgtt
    11341 gctgcaagca agccagaatt catcaaacca gaagacgtat ccgctgaagt ggtagaaaaa
    11401 gaataccagg tacagctgga tatcgcgatg cagtctggta agccgaaaga aatcgcagag
    11461 aaaatggttg aaggccgcat gaagaaattc accggcgaag tttctctgac cggtcagccg
    11521 ttcgttatgg aaccaagcaa aactgttggt cagctgctga aagagcataa cgctgaagtg
    11581 actggcttca tccgcttcga agtgggtgaa ggcatcgaga aagttgagac tgactttgca
    11641 gcagaagttg ctgcgatgtc caagcagtct taattatcaa aaaggagccg cctgagggcg
    11701 gcttcttttt gtgcccatct tgtaaattca gctaaccctt gtggggctgc gctgaaaagc
    11761 gacgtacaat gtcgctagta ttaattcatt tcaatcgttg acagtctcag gaaagaaaca
    11821 tggctaccaa tgcaaaaccc gtctataaac gcattctgct taagttgagt ggcgaagctc
    11881 tgcagggcac tgaaggcttc ggtattgatg caagcatact ggatcgtatg gctcaggaaa
    11941 tcaaagaact ggttgaactg ggtattcagg ttggtgtggt gattggtggg ggtaacctgt
    12001 tccgtggcgc tggtctggcg aaagcgggta tgaaccgcgt tgtgggcgac cacatgggga
    12061 tgctggcgac cgtaatgaac ggcctggcaa tgcgtgatgc actgcaccgc gcctatgtga
    12121 acgctcgtct gatgtccgct attccattga atggcgtgtg cgacagctac agctgggcag
    12181 aagctatcag cctgttgcgc aacaaccgtg tggtgatcct ctccgccggt acaggtaacc
    12241 cgttctttac caccgactca gcagcttgcc tgcgtggtat cgaaattgaa gccgatgtgg
    12301 tgctgaaagc aaccaaagtt gacggcgtgt ttaccgctga tccggcgaaa gatccaaccg
    12361 caaccatgta cgagcaactg acttacagcg aagtgctgga aaaagagctg aaagtcatgg
    12421 acctggcggc cttcacgctg gctcgtgacc ataaattacc gattcgtgtt ttcaatatga
    12481 acaaaccggg tgcgctgcgc cgtgtggtaa tgggtgaaaa agaagggact ttaatcacgg
    12541 aataattccc gtgatggata aataagggta agattccgcg taagtatcgc gggggcgtaa
    12601 gtctggttat aaggcgttat tgttgcaggc agtttggtca cggccagcgc gcagcaaccg
    12661 gagcgtacaa aagtacgtga ggatggcgag cactgctcgg ggccaaaatg gcaaataaaa
    12721 tagcctaata atccagacga ttacccgtaa tatgtttaat cagggctata cttagcacac
    12781 ttccactgtg tgtgactgtc tggtctgact gagacaagtt ttcaaggatt cgtaacgtga
    12841 ttagcgatat cagaaaagat gctgaagtac gcatggacaa atgcgtagaa gcgttcaaaa
    12901 cccaaatcag caaaatacgc acgggtcgtg cttctcccag cctgctggat ggcattgtcg
    12961 tggaatatta cggcacgccg acgccgctgc gtcagctggc aagcgtaacg gtagaagatt
    13021 cccgtactct gaaaatcaac gtgtttgatc gttcaatgtc tccggccgtt gaaaaagcga
    13081 ttatggcgtc cgatcttggc ctgaacccga actctgcggg tagcgacatc cgtgttccgc
    13141 tgccgccgct gacggaagaa cgtcgtaaag atctgaccaa aatcgttcgt ggtgaagcag
    13201 aacaagcgcg tgttgcagta cgtaacgtgc gtcgtgacgc gaacgacaaa gtgaaagcac
    13261 tgttgaaaga taaagagatc agcgaagacg acgatcgccg ttctcaggac gatgtacaga
    13321 aactgactga tgctgcaatc aagaaaattg aagcggcgct ggcagacaaa gaagcagaac
    13381 tgatgcagtt ctgatttctt gaacgacaaa aacgccgctc agtagatcct tgcggatcgg
    13441 ctggcggcgt tttgcttttt attctgtctc aactctggat gtttcatgaa gcaactcacc
    13501 attctgggct cgaccggctc gattggttgc agcacgctgg acgtggtgcg ccataatccc
    13561 gaacacttcc gcgtagttgc gctggtggca ggcaaaaatg tcactcgcat ggtagaacag
    13621 tgcctggaat tctctccccg ctatgccgta atggacgatg aagcgagtgc gaaacttctt
    13681 aaaacgatgc tacagcaaca gggtagccgc accgaagtct taagtgggca acaagccgct
    13741 tgcgatatgg cagcgcttga ggatgttgat caggtgatgg cagccattgt tggcgctgct
    13801 gggctgttac ctacgcttgc tgcgatccgc gcgggtaaaa ccattttgct ggccaataaa
    13861 gaatcactgg ttacctgcgg acgtctgttt atggacgccg taaagcagag caaagcgcaa
    13921 ttgttaccgg tcgatagcga acataacgcc atttttcaga gtttaccgca acctatccag
    13981 cataatctgg gatacgctga ccttgagcaa aatggcgtgg tgtccatttt acttaccggg
    14041 tctggtggcc ctttccgtga gacgccattg cgcgatttgg caacaatgac gccggatcaa
    14101 gcctgccgtc atccgaactg gtcgatgggg cgtaaaattt ctgtcgattc ggctaccatg
    14161 atgaacaaag gtctggaata cattgaagcg cgttggctgt ttaacgccag cgccagccag
    14221 atggaagtgc tgattcaccc gcagtcagtg attcactcaa tggtgcgcta tcaggacggc
    14281 agtgttctgg cgcagctggg ggaaccggat atgcgtacgc caattgccca caccatggca
    14341 tggccgaatc gcgtgaactc tggcgtgaag ccgctcgatc tttgcaaact aagtgcgttg
    14401 acatttgccg caccggatta tgatcgttat ccatgcctga aactggcgat ggaggcgttc
    14461 gaacaaggtc aggcagcgac gacagcattg aatgccgcaa acgaaatcac cgttgctgct
    14521 tttcttgcgc aacaaatccg ctttacggat atcgctgcgt tgaatttatc ggtactggaa
    14581 aaaatggata tgcgcgaacc acaatgtgtg gacgatgtgt tatctgttga tgcgaacgcg
    14641 cgtgaagtcg ccagaaaaga ggtgatgcgt ctcgcaagct gaggataatc cggctacaga
    14701 gagtctcgct atttgttagc gtagggcttc agtgatatag tctgcgccat ctgatcgtaa
    14761 gtagttggct ttataaggtc aggtatgccg tggttttgca cggctttttt ttgtataggc
    14821 ttcagtattc ctgagtaccg taaaccctgt cagggaataa aaaacgcgtg atgttgtctg
    14881 ctactcaacc acttagcgaa aaattgccag cgcatggctg ccgtcatgtt gcgatcatta
    14941 tggacggcaa tggtcgctgg gcaaaaaagc aagggaagat tcgtgccttt gggcataaag
    15001 ccggggcaaa atccgtccgc cgggctgtct cttttgcggc caacaacggt attgaggcgt
    15061 taacgctgta tgcctttagt agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt
    15121 taatggaact gtttgtgtgg gcgctcgata gcgaagtaaa aagtctgcac cgacataacg
    15181 tgcgtctgcg tattattggc gataccagtc gctttaactc gcgtttgcaa gaatgtattc
    15241 gtaaatctga agcgctaaca gccgggaata ccggtctgac gctgaatatt gcggcgaact
    15301 acggtggacg ttgggatata gtccagggag tcaggcaact ggctgaaaag gtgcagcaag
    15361 gaaacctgca accagatcag atagatgaag agatgctaaa ccagcatgtc tgtatgcatg
    15421 aactggcccc tgtagattta gtaattagga ctggggggga gcatcgcatt agtaactttt
    15481 tgctttggca aattgcctat gccgaacttt actttacaga tgttctctgg cccgatttcg
    15541 atgaacaaga ctttgaaggg gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg
    15601 gcaccgagcc cggtgatgaa acagcctgat ggggggcgct tttgctgaag tatcgcctga
    15661 tatctgcttt tgtgttaata cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg
    15721 ggttcgccat tgtaacgctg gtggtctgca tgctggcagc gtgggaatgg ggacagctta
    15781 gcggttttac cactcgttcg cagcgagtat ggttggcggt gttatgcggg ttattgttgg
    15841 cgctgatgct ttttctgttg ccggaatatc accgaaatat tcatcaaccg ctggttgaaa
    15901 tctcactttg ggcttcgctg ggttggtgga ttgtcgcgct attgctggtg ctgttttacc
    15961 caggttccgc agcaatctgg cgtaactcta aaacattgcg ccttattttt ggcgtgctaa
    16021 ccattgttcc cttcttctgg ggcatgctgg cgttacgggc ctggcactat gacgagaatc
    16081 attacagtgg cgcaatatgg ctgctctatg tcatgatcct ggtatggggc gctgactccg
    16141 gcgcatatat gtttggcaaa ttgtttggta aacataagct ggcaccgaag gtttctccgg
    16201 gtaaaacctg gcaaggcttt ataggtggac tcgctactgc agcggtaatc tcatggggtt
    16261 atggcatgtg ggcgaatctc gacgtcgctc ccgtcacctt actcatttgc tctattgtcg
    16321 cagcgttagc ctcagtgctc ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa
    16381 ttaaggacag cggtcattta attccaggac acggtggtat tttagatcgt attgatagcc
    16441 tgacggctgc ggtaccggtc tttgcttgct tgttgttact ggtattcagg acgctttaac
    16501 ggaaggtaat atgctgagtt ttctctggga tttggcttcg ttcatcgttg cactgggtgt
    16561 acttatcacc gtgcatgaat ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt
    16621 tgagcgtttc tcaatagggt ttggtaaggc gctctggcgg cgaactgata agctcggcac
    16681 cgaatatgtt atcgccctga tcccgctggg cggttatgtc aaaatgctgg atgagcgcgc
    16741 agaaccggtc gttccggaac tccgccacca tgccttcaat aataaatctg ttggccaacg
    16801 agcggcgatt attgccgcag gtccggttgc aaacttcatt tttgctatct ttgcctactg
    16861 gctggttttt attattggtg tgcctggcgt acgtccggtg gttggtgaaa tagcagccaa
    16921 ttcgatagct gcggaagcac aaattgcacc aggtacggaa ctaaaagccg tagatggtat
    16981 cgaaacgcct gattgggatg ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag
    17041 caccaccatt acagtagcgc catttggcag cgaccaacgg cgggatgtaa agctcgattt
    17101 acgtcactgg gcgtttgagc ctgataaaga agatccggta tcttcgctgg ggattcgtcc
    17161 tcgtgggctg caaattgaac ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa
    17221 ggcaggtttg caagcaggcg acaggatcgt taaagtcgat ggtcagccct taacgcagtg
    17281 ggtgaccttt gtgatgcttg tccgggataa cccgggtaaa tccttagcgt tagaaatcga
    17341 aaggcaggga agtcccttgt ctttgacatt aatcccggag agtaaaccgg gtaatggtaa
    17401 agcgattggt tttgtcggta ttgagccgaa agtcattcct ttgccagatg agtataaagt
    17461 tgtacgccag tatgggccgt tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct
    17521 gatgaagctg acggtcagta tgctgggaaa attgatcacc ggtgatgtga aactgaacaa
    17581 cctcagtggg ccgatctcta tcgccaaggg ggctgggatg acagcggaac tcggggtagt
    17641 ttattacctg ccgtttcttg cgcttattag cgtgaactta gggataatta acctgtttcc
    17701 gttgcccgta cttgacgggg cgcatctgct gttccttgcg atcgaaaaga tcaagggcgg
    17761 accggtatcc gagcgggttc aagacttttg ttatcgcatt ggctcgattc tgctggtgct
    17821 gttaatgggg cttgcacttt tcaatgattt ctctcggtta tgagagttag ttaggaagaa
    17881 cgcataataa cgatggcgat gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc
    17941 accgtatacg gtgctgaagg ggtcgtagtg aaagatattc atttcgaagg ccttcagcgt
    18001 gtcgccgttg gtgcggccct cctcagtatg ccggtgcgta caggcgacac ggttaatgat
    18061 gaagatatca gtaataccat tcgcgctctg tttgctaccg gcaactttga ggatgttcgc
    18121 gtccttcgtg atggtgatac ccttctggtt caggtaaaag aacgtccgac cattgccagc
    18181 attactttct ccggtaacaa atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct
    18241 tctggtgtgc gtgtgggcga atccctcgat cgcaccacca ttgccgatat cgagaaaggt
    18301 ctggaagact tctactacag cgtcggtaaa tatagcgcca gcgtaaaagc tgtcgtgacc
    18361 ccgctgccgc gcaaccgtgt tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa
    18421 atccagcaaa ttaacattgt tggtaaccat gctttcacca ccgacgaact gatctctcat
    18481 ttccaactgc gtgacgaagt gccgtggtgg aacgtggtag gcgatcgtaa ataccagaaa
    18541 cagaaactgg cgggcgacct tgaaaccctg cgcagctact atctggatcg cggttatgcc
    18601 cgtttcaaca tcgactctac ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc
    18661 acggtgaaca ttaccgaagg cgatcagtac aagctttctg gcgttgaagt gagcggcaac
    18721 cttgccgggc actccgctga aattgagcag ctgactaaga tcgagccggg cgagctgtat
    18781 aacggcacca aagtgaccaa gatggaagat gacatcaaaa agcttctcgg tcgctatggt
    18841 tatgcctatc cgcgcgtaca gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa
    18901 ttacgcgtga acgttgatgc gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt
    18961 aacgatacct cgaaagatgc cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg
    19021 ctgggaagcg atctggtcga tcagggtaag gagcgtctga atcgtctggg cttctttgaa
    19081 actgtcgata ccgataccca acgtgttccg ggtagcccgg accaagtaga tgtagtctac
    19141 aaggtgaaag aacgtaacac cggtagcttc aacttcggga ttggttacgg tactgaaagt
    19201 ggcgtgagct tccaggctgg cgtgcaacag gataactggt taggtacagg ttatgctgtt
    19261 ggtatcaacg ggaccaaaaa cgattaccag acctatgctg aattgtcggt aaccaacccg
    19321 tacttcactg tagatggtgt aagcctcggt ggtcgtctct tctataatga cttccaggca
    19381 gatgacgccg acctgtccga ctataccaac aagagttatg gtacagacgt gacgttgggc
    19441 ttcccgatca acgaatataa ctcgctgcgt gcaggtctgg gttatgtaca taactccctg
    19501 tccaacatgc agcctcaggt tgcgatgtgg cgttatctgt actctatggg tgaacatccg
    19561 agcacctctg atcaggataa cagcttcaaa acggacgact tcacgttcaa ctatggttgg
    19621 acctataaca agcttgaccg tggttacttc ccgacagatg gttcacgtgt caacctgacc
    19681 ggtaaagtga ccattcctgg atcggataac gaatactaca aagtgacgtt agacacggcg
    19741 acttatgtgc cgatcgatga cgatcacaaa tgggttgttc tggggcgtac ccgctggggt
    19801 tatggtgatg gtttaggcgg caaagagatg ccgttctacg agaacttcta tgccggtggt
    19861 tccagcaccg tccgtggctt ccagtccaat accattggtc cgaaagcagt ttacttcccg
    19921 catcaggcca gtaattatga tccggactat gattacgaat gtgcgactca ggacggcgcg
    19981 aaagacctgt gtaaatcgga tgatgctgta ggcggtaacg ccatggcggt tgccagcctc
    20041 gagttcatca ccccgacgcc gtttattagc gataagtatg ctaactcggt tcgtacttcc
    20101 ttcttctggg atatgggtac cgtttgggat acaaactggg attccagcca atattctgga
    20161 tatccggact atagtgatcc aagcaatatc cgtatgtctg cgggtatcgc attacaatgg
    20221 atgtccccat tggggccgtt ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga
    20281 gacaaggcag aacagttcca gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag
    20341 gaatgtagtg gtagtgtagc gatgacttta ggcgatcaat ataagatcgc cgggccacgc
    20401 aaagaactgc accctccggt gcaaatggga tggtaaggag tttattgtga aaaagtggtt
    20461 attagcggca ggtctcggtt tagcactggc aacttctgct caggcggctg acaaaattgc
    20521 aatcgtcaat atgggcagcc tgttccagca ggtagcgcag aaaaccggtg tttctaacac
    20581 gctggaaaat gagttcaaag gccgtgccag cgaactgcag cgtatggaaa ccgatctgca
    20641 ggctaaaatg aaaaagctgc agtccatgaa agcgggcagc gatcgcacta agctggaaaa
    20701 agacgtgatg gctcagcgcc agacttttgc tcagaaagcg caggcttttg agcaggatcg
    20761 cgcacgtcgt tccaacgaag aacgcggcaa actggttact cgtatccaga ctgctgtgaa
    20821 atccgttgcc aacagccagg atatcgatct ggttgttgat gcaaacgccg ttgcttacaa
    20881 cagcagcgat gtaaaagaca tcactgccga cgtactgaaa caggttaaat aagtaatgcc
    20941 ttcaattcga ctggctgatt tagcgcagca gttggatgca gaactacacg gtgatggcga
    21001 tatcgtcatc accggcgttg cgtccatgca atctgcacaa acaggtcaca ttacgttcat
    21061 ggttaaccca aaataccgtg agcatttagg cttgtgccag gcgtccgcgg ttgtcatgac
    21121 ccaggacgat cttcctttcg cgaaaagtgc cgcgctggta gtgaagaatc cctacctgac
    21181 ttacgcgcgc atggcgcaaa ttttagatac cacgccgcag cccgcgcaga acattgcacc
    21241 cagtgcggtg atcgacgcga cggcgaagct ggggaacaat gtatcgattg gcgctaacgc
    21301 ggtgattgaa tccggcgttg aactgggcga taacgtgatt atcggtgccg gttgcttcgt
    21361 aggtaaaaac agcaaaatcg gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca
    21421 tgagatccag atcggtcaga attgcctgat ccagtccgga acagtggtag gcgcagacgg
    21481 ctttggttat gccaacgatc gtggtaactg ggttaagatc ccacagattg gtcgcgtaat
    21541 tattggcgat cgcgtggaga tcggtgcctg tactaccatc gatcgcggcg cgctggatga
    21601 cactgttatt ggcaatggcg tgatcattga taaccagtgc cagattgcac ataacgtcgt
    21661 gattggcgac aatacggcgg ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg
    21721 tcgttactgc atgatcggcg gagccagcgt aatcaacggg catatggaaa tatgcgacaa
    21781 agtgacggtt acgggcatgg gtatggtgat gcgtcccatc actgaaccag gcgtctattc
    21841 ctcaggcatt ccgctgcaac ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa
    21901 cattgatgac atgagcaagc gtctgaaatc gcttgagcgc aaggttaatc aacaagacta
    21961 acgttccatc ttttgttcgc caaactttac ggcctgtctc attcttacga ttgcggcagg
    22021 ccgtgttatt attgtcgttt cttatatttt gacaggaaga gtatcttgac tactaacact
    22081 catactctgc agattgaaga gattttagaa cttctgccgc accgtttccc gttcttactg
    22141 gtggatcgcg tgctggattt tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct
    22201 gtcaatgagc cattcttcca gggccatttc cctggaaaac cgattttccc gggtgtgctg
    22261 attctggaag caatggcaca ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg
    22321 gaaccgggtg agctgtacta cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc
    22381 gtgcctggcg atcaaatgat catggaagtc actttcgaaa aaacgcgccg cggcctgacc
    22441 cgttttaaag gggttgctct ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt
    22501 gctcgtagcc gggaggcctg atacgtgatt gataaatccg cctttgtgca tccaaccgcc
    22561 attgtggaag agggcgcgtc aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt
    22621 ggaccccatg tcggaattgg tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt
    22681 catactaaaa ttggccgcga taatgagatt tatcagttcg cctccatcgg cgaagttaac
    22741 caggatctga aatatgctgg cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt
    22801 cgcgaaagcg tcaccattca tcgtggcaca gtccagggcg gtggattgac gaaggtgggc
    22861 agcgacaact tactgatgat caacgcgcac attgcgcacg attgtacggt aggtaaccgc
    22921 tgtattctcg ccaacaacgc aacgctggcg ggtcacgtat cggttgacga cttcgcgatc
    22981 atcggcggca tgaccgcagt ccatcagttc tgcatcattg gtgcgcacgt gatggttggc
    23041 ggctgctccg gtgtggcgca ggacgtccct ccttatgtca ttgcgcaggg taaccacgca
    23101 acgccgttcg gtgtcaatat cgaagggctg aagcgccgcg gattcagccg tgaggcgatt
    23161 accgctatcc gcaatgcgta taagctgatt tatcgtagcg gtaaaacgct cgatgaagtg
    23221 aaaccggaaa ttgctgaact ggcggaaaca tatccggaag tgaaagcctt taccgatttc
    23281 ttttcacgct caacgcgcgg tctgattcgt taatgactga acagcgtcca ttaacgattg
    23341 ccctggtcgc cggagaaacc tccggcgata tcctgggggc cggtttaatc cgcgctctga
    23401 aagaacgtgt gcccaacgcc cgctttgttg gtgttgccgg gccacgaatg caggctgaag
    23461 gctgcgaagc ctggtacgaa atggaagaac tggcggtgat gggcattgtt gaagtgctcg
    23521 gtcgtctgcg tcgcttactg catattcgtg ccgatctgac aaagcgtttt ggcgaactga
    23581 agccagatgt ttttgttggt attgatgcgc ctgacttcaa tattactctt gaaggtaacc
    23641 tcaaaaagca gggtatcaaa accattcatt acgtcagtcc gtcagtctgg gcgtggcgac
    23701 agaaacgtgt tttcaaaata ggcagagcca ccgatctggt gctcgcattt ctgcctttcg
    23761 aaaaagcgtt ttatgacaaa tacaacgtac cgtgccgctt tatcggtcat accatggctg
    23821 atgccatgcc attagatcca gataaaaatg ccgcccgtga tgtgctgggg atccctcacg
    23881 atgcccactg cctggcgttg ctaccgggga gccgtggtgc agaagttgaa atgcttagtg
    23941 ccgatttcct gaaaacggcc cagcttttgc gccagacata tccggatctc gaaatcgtgg
    24001 tgccactggt gaatgccaaa cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc
    24061 cagacttttc agttcatttg ctggatggga tgggccgtga ggcgatggtc gccagcgatg
    24121 cggcgctact ggcgtcgggt acggcagccc tggagtgtat gctggcgaaa tgcccgatgg
    24181 tggtgggata tcgcatgaag ccttttacct tctggttggc gaagcggctg gtgaaaactg
    24241 attatgtctc gctgccaaat ctgctggcgg gcagagagtt agtcaaagag ttattgcagg
    24301 aagagtgtga gccgcaaaaa ctggctgcgg cgctgttacc gctgttggcg aacgggaaaa
    24361 tcagccacgc gatgcacgat accttccgtg aactgcatca gcagatccgc tgcaatgccg
    24421 atgagcaggc ggcacaagcc gttctggagt tagcacaatg atcgaatttg tttatccgca
    24481 cacgcagctg gttgcgggtg tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt
    24541 caccgctgcg gtgatccttg acccggcgcg cccgattgcc gggctgaatg attccaaaaa
    24601 gctgagcgaa aaacgccgtc tggcgctctg tgaagagatc aaagagaaag cgttgagctg
    24661 gagtctgggc cgcgcggaac cccacgaaat cgacgagctg aacattcttc atgcgaccat
    24721 gctggcgatg cagcgtgccg tcgctgggct gcatattgcg ccggaatatg tgttgattga
    24781 tggtaaccgc tgcccgaaat taccgatgcc tgcgatggct gtggtgaaag gcgatagccg
    24841 cgtaccggaa atcagtgccg cgtctatcct ggcgaaagtg acgcgtgacg ccgaaatggc
    24901 ggcgctggat attgttttcc cgcaatatgg ttttgcccaa cacaaagggt acccaaccgc
    24961 ttttcatctg gaaaaactgg ctgaacacgg cgcgaccgaa caccatcggc gcagctttgg
    25021 gcctgtcaaa cgcgcactgg gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg
    25081 gaatctgaag atgtctgaac cacgtttcgt acacctgcgg gtgcacagcg actactcgat
    25141 gatcgatggc ctggccaaaa ctgcaccgtt ggtaaaaaag gcggcggcgt tgggtatgcc
    25201 agcactggcg atcaccgatt tcaccaacct ttgtggtctg gtgaagttct acggagcggg
    25261 acatggcgca gggattaagc ctatcgtcgg ggcagatttt aacgtccagt gcgacctgct
    25321 gggtgatgag ttaacccacc tgacggtact ggcggcgaac aataccggct atcagaatct
    25381 gacgttgctg atctcaaaag cgtatcagcg cgggtacggt gccgctgggc cgatcatcga
    25441 tcgcgactgg cttatcgaat taaatgaagg gttgatcctt ctttccggcg ggcgcatggg
    25501 cgacgtcgga cgcagtcttt tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt
    25561 ttatgaagaa cacttcccgg atcgctattt tctcgagctg atccgcaccg gcaggccgga
    25621 tgaagaaagc tatctgcacg cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt
    25681 ggcgactaac gatgtgcgct ttatcgacag cagcgacttt gacgcacacg aaatccgcgt
    25741 cgcgatccac gacggcttta ccctcgacga tcctaaacgc ccgcgtaact attcgccgca
    25801 gcaatatatg cgtagcgaag aggagatgtg tgagctgttt gccgacatcc ccgaagccct
    25861 tgccaacacc gttgagatcg ccaaacgctg taacgtaact gtgcgtcttg gtgaatactt
    25921 cctgccgcag ttcccgaccg gggacatgag caccgaagat tatctggtca agcgtgcaaa
    25981 agagggcctg gaagagcgtc tggccttttt attccctgac gaggaagaac gtgttaagcg
    26041 ccgcccggaa tatgacgaac gtctggagac tgaacttcag gttatcaacc agatgggctt
    26101 cccgggctac ttcctcatcg ttatggaatt tatccagtgg tcgaaagata acggcgtacc
    26161 ggtagggcca ggccgtggct ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac
    26221 cgacctcgat ccgctggaat ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt
    26281 ctccatgcct gacttcgacg ttgacttctg tatggagaaa cgcgatcagg ttatcgagca
    26341 cgtagcggac atgtacggtc gtgatgcggt atcgcagatc atcaccttcg gtacaatggc
    26401 gtcgaaagcg gtgatccgcg acgtaggccg cgtgctgggg catccgtacg gctttgtcga
    26461 tcgtatctcg aaactgatcc cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc
    26521 cgagccgcag ctgccggaaa tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat
    26581 ggcgcgcaaa ctggaagggg tcacccgtaa cgccggtaag cacgccggtg gggtggttat
    26641 cgcgccgacc aaaattaccg attttgcgcc gctttactgc gatgaagagg gcaaacatcc
    26701 ggtcacccag tttgataaaa gcgacgttga atacgccgga ctggtgaagt tcgacttcct
    26761 tggtttgcgt acgctcacca tcatcaactg ggcgctggag atgatcaaca agcggcgggc
    26821 gaagaatggc gagccgccgc tggatatcgc cgcgatcccg ctggatgaca agaaaagctt
    26881 cgacatgctg caacgctcgg aaaccacggc ggtattccag cttgaatcgc gcggcatgaa
    26941 ggacctgatc aagcgtctgc aacctgactg cttcgaagat atgatcgcac tggtggcgct
    27001 gttccgccca ggtccgttgc aatcagggat ggtggataac tttatcgacc gtaaacatgg
    27061 tcgcgaagaa atctcctatc cggacgtaca gtggcagcat gaaagcctga aaccggtact
    27121 ggagccaacc tacggcatca tcctgtatca ggaacaggtc atgcagattg cgcaggtgct
    27181 ttctggttat accctcggtg gcgcggatat gctgcgtcgt gcgatgggta agaaaaagcc
    27241 ggaagagatg gctaagcagc gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa
    27301 cgccgaactg gcgatgaaaa tcttcgacct ggtggagaaa ttcgcgggtt acggatttaa
    27361 caaatcgcac tctgcggcct atgctttggt gtcatatcaa acgttatggc tgaaagcgca
    27421 ctatccggcg gagtttatgg cggcggtaat gaccgccgat atggacaaca ccgagaaggt
    27481 ggtgggcctg gtggatgagt gctggcggat ggggctgaaa atcctgccac cagatataaa
    27541 ctccggtctt taccatttcc acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc
    27601 catcaaaggg gtaggtgaag gtccgattga ggccatcatc gaagcccgta ataaaggcgg
    27661 ctacttccgc gaactgtttg atctctgcgc ccgtaccgac accaaaaagt taaaccggcg
    27721 ggtgctggaa aaactgatca tgtccggggc gtttgaccgt cttgggccac accgcgcggc
    27781 gctgatgaac tcgctgggtg atgcgttaaa agcggcagat caacacgcga aagcggaagc
    27841 tatcggtcag gccgatatgt tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc
    27901 ctacgccagc tgccaaccgt ggccggagca ggtggtgtta gatggggaac gtgaaacgtt
    27961 aggtctgtac cttacgggac accctatcaa ccagtattta aaagagattg agcgttatgt
    28021 cggaggcgta aggctgaaag acatgcaccc gacagaacgt ggtaaagtca tcacggctgc
    28081 ggggctcgtt gttgccgcgc gggttatggt caccaagcgc ggcaatcgta tcggtatctg
    28141 cacgctggat gaccgttccg ggcggctgga agtgatgttg tttactgacg ccctggataa
    28201 ataccagcaa ttgctggaaa aagaccgcat acttatcgtc agcggacagg tcagctttga
    28261 tgacttcagc ggtgggctta aaatgaccgc tcgcgaagtg atggatattg acgaagcccg
    28321 agaaaaatat gctagcgggc ttgctatctc gctgacggac aggcaaattg atgaccagct
    28381 tttaaaccga ctccgtcagt ctctggaacc ccaccgctct gggacaattc cagtacatct
    28441 ctactatcag agggcggatg cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtcta
    28501 tccgagcgat cgtttattaa acgatctccg tggcctcatt ggttcggagc aggtggaact
    28561 ggagtttgac taatacagga atactatgag tctgaatttc cttgattttg aacagccgat
    28621 tgcagagctg gaagcgaaaa tcgattctct gactgcggtt agccgtcagg atgagaaact
    28681 ggatattaac atcgatgaag aagtgcatcg tctgcgtgaa aaaagcgtag aactgacacg
    28741 taaaatcttc gccgatctcg gtgcatggca gattgcgcaa ctggcacgcc atccacagcg
    28801 tccttatacc ctggattacg ttcacctggc atttgatgaa tttgacgaac tggctggcga
    28861 ccgcgcgtat gcagacgata aagctatcgt cggtggtatc gcccgtcttg acggtcgtcc
    28921 ggtgatgatc attggtcatc aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt
    28981 tggtatgcca gcgccagaag gttaccgcaa agcactgcgt ctgatgcaaa tggctgaacg
    29041 ctttaagatg cctatcatca cctttatcga caccccgggg gcttatcctg gcgtgggcgc
    29101 agaagagcgt ggtcagtctg aagccattgc acgcaacctg cgtgaaatgt ctcgcctcgg
    29161 cgtaccggta gtttgtacgg ttatcggtga aggtggttct ggcggtgcgc tggcgattgg
    29221 cgtgggcgat aaagtgaata tgctgcaata cagcacctat tccgttatct cgccggaagg
    29281 ttgtgcgtcc attctgtgga agagcgccga caaagcgccg ctggcggctg aagcgatggg
    29341 tatcatcgct ccgcgtctga aagagctgaa actgatcgac tccatcatcc cggaaccgct
    29401 gggcggcgct caccgtaacc cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc
    29461 ggatctggcc gatctcgatg tgttaagcac tgaagattta aaaaatcgtc gttatcagcg
    29521 cctgatgagc tacggttacg cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg
    29581 ccctttttta tcgccacggt ttgagcaggc tatgattaag gaaggatttt ccaggaggaa
    29641 cacatgaaca tcattgccat tatgggaccg catggcgtct tttataaaga tgagcccatc
    29701 aaagaactgg agtcggcgct ggtggcgcaa ggctttcaga ttatctggcc acaaaacagc
    29761 gttgatttgc tgaaatttat cgaacataac cctcgaattt gcggcgtgat ttttgactgg
    29821 gatgagtaca gtctcgattt atgcagcgat atcaatcaac ttaatgaata tctcccgctt
    29881 tatgccttca tcaacaccca ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg
    29941 ctctggtttt ttgaatatgc gctggggcag gcggaagata tcgccattcg tatgcgtcag
    30001 tacaccaacg aatatcttga taacattacg ccgccgttca cgaaagcctt gtttacctac
    30061 gttaaagagc ggaagtacac cttttgtacg ccggtgcata tgggcggcac cgcatatcaa
    30121 aaaagcccgg ttggctgcct gttttatgat tttttcggcg ggaataccct caaggctgat
    30181 gtctctattt cggtcaccga gcttggttcg ttgctcgacc acaccgggcc acacctggaa
    30241 gcggaagagt acatcgcgcg gacttttggc gcggaacaga gttatatcgt taccaacgga
    30301 acatcgacgt cgaacaaaat tgtgggtatg tacgccgcgc catccggcag tacgctgttg
    30361 atcgaccgca attgtcataa atcgctggcg catctgttga tgatgaacga tgtagtgcca
    30421 gtctggctga aaccgacgcg taatgcgttg gggattcttg gtgggatccc gcaccgtgaa
    30481 tttactcgcg acagcatcga agagaaagtc gctgccacca cgcaggcaca atggccggtt
    30541 catgcggtga ttaccaactc cacctatgat ggcttgctct acaacaccga ctggatcaaa
    30601 cagacgctgg atgtcccgtc gatccacttc gattccgcct gggtgccgta cacccatttt
    30661 catccgatct accaggggaa aagtggtatg agcggcgagc gtgttgcggg aaaagtgatc
    30721 ttcgaaacgc aatcgaccca caaaatgctg gcggcgttat cgcaggcttc gctgatccac
    30781 attaaaggcg agtatgacga agaggcgttt aacgaagcct ttatgatgca taccaccacc
    30841 tcgcccagtt atcccattgt tgcttcggtt gagacggcgg cggcgatgct gcgtggtaat
    30901 ccgggcaaac ggctgattaa ccgttcggtg gagcgggcgc tgcattttcg caaagaggtc
    30961 cagcggttgc gggaagagtc tgacggttgg tttttcgata tctggcaacc gccgcaggtg
    31021 gatgaagccg aatgctggcc cgttgcgcca ggcgaacagt ggcacggctt taacgatgcg
    31081 gatgccgatc atatgtttct cgatccggtt aaagtcacta ttttgacacc ggggatggac
    31141 gaacagggca atatgagcga ggaggggatc ccggcggcgc tggtggcaaa attcctcgac
    31201 gaacgtggga tcgtagtaga gaaaaccggc ccttataacc tgctgtttct ctttagtatt
    31261 ggtatcgata aaaccaaagc aatgggatta ttgcgtgggt tgacagaatt taaacgctct
    31321 tacgatctca acctgcggat caaaaatatg ctacccgatc tctatgctga agatcccgat
    31381 ttctaccgca atatgcgtat tcaggatctg gcgcaaggga tccataagct gattcgtaaa
    31441 cacgatcttc ccggtttgat gttgtgggca ttcgatactt tgccggaaat gatcatgacg
    31501 ccacatcagg catggcagag acagattaaa ggtgaagtag aaaccattgc gctggaacaa
    31561 ctggtcggta gagtatcggc aaatatgatc ctgccttatc caccgggcgt accgctgttg
    31621 atgccaggag aaatgctgac caaagagagc cgcacggtac tcgattttct actgatgctt
    31681 tgttccgtag ggcaacatta ccccggtttt gaagcggata ttcacggcgc gaaacaggac
    31741 gaagacggcg tttaccgcgt acgagtccta aaaatggcgg gataacttgc cagagcggct
    31801 tacgggcgag taacgtgctg ttaacaaata aaggagacgt tatgctgggt ttaaaacagg
    31861 ttcaccatat tgcgattatt gcgacggatt atgcggtgag caaagctttc tactgcgata
    31921 ttcttggttt cacgctgcaa agcgaagtct atcgcgaagc gcgcgactcg tggaaagggg
    31981 atttggcgct taatgggcaa tatgtgattg agcttttctc ttttccgttc ccgccggagc
    32041 gccccagccg accggaagct tgcggcctgc gtcatctggc ttttagcgtt gatgacatcg
    32101 atgcggcagt ggcgcacctt gaaagccata acgtgaagtg tgaagccatc cgtgtcgatc
    32161 catacacgca aaaacgcttc accttcttta acgatccgga cgggctgccg ttggaactgt
    32221 atgagcagta aggcttgtca tcgccgcatt tgcccggtaa cgtgccgggc attgctactg
    32281 taaaatcgca ccatcatgac actcacgctc aatagacaac ttctcacctc acgccagatt
    32341 ttggtcgcct ttagcggcgg gcttgactcc accgttctgc tgcatcagtt ggtgcagtgg
    32401 cggacggaaa atccgggcgt cactctgcgc gctattcatg tgcatcacgg tttaagtgcc
    32461 aatgccgatg cctgggttac gcattgcgaa aacgtctgcc aacagtggca ggtgccgctg
    32521 gtggtcgaac gcgtacaact tgcgcaagag gggctgggga ttgaggccca ggcgcggcag
    32581 gcgcgttatc aggcatttgc ccgtacccta ttgcccggcg aagtgctggt caccgcgcaa
    32641 catctcgacg atcaatgtga aacctttctg ctggcgctaa aacgcggtag cggccctgcc
    32701 gggctttcgg ctatggcgga agtttcggag tttgccggaa cacggcttat ccgcccgttg
    32761 cttgcccgca cgcgggggga actggcgcag tgggcattgg cgcatggttt acgctggatt
    32821 gaagacgaaa gtaatcagga cgactcatac gatcgtaact ttctgcgcct gcgcgtagtg
    32881 ccgttattgc agcagcgttg gccgcatttt gcggaagcaa cggcccgcag cgccgcactt
    32941 tgtgctgaac aagagagtct gttggatgaa ctgctggcgg atgatttagc acactgccaa
    33001 acgtcgcagg ggacgctgca gattgcgcca atgctggcga tgagtgatgc ccgccgcgcg
    33061 gcgattatcc gtcgctggct ggcagggcag aatgcaccaa tgccttcccg cgacgcgctg
    33121 gtgcggatct ggcaggaagt ggcgctggcg cgggaagatg cctccccctg tttacgcttg
    33181 ggcgcgtttg aaatccgccg ctatcagtcg caactgtggt ggattaaatc cgtcaccggg
    33241 caaagtgaaa ccattatgcc gtggcagacg tggctacaac cgctggaatt accggcgggg
    33301 ctgggaagcg tacagcttac tgcgggaggc gatattcgcc ctccgcgtgc agacgaagcg
    33361 gtcagcgtgc gtttcaaatc ggcaggattg ctgcatattg tcgggcgcaa cggcggacgt
    33421 aagctgaaga aaatctggca agagttaggc gtgccgccgt ggctacgtga caccacgcca
    33481 ctgctgtttt atggcgaaac gctgattgcg gcggcagggg tatttgtgac gcaagaaggt
    33541 gtggctgaag gtgagaatgg cgtaagtttt gtctggcaga aaacgcttag ttaagtgaaa
    33601 gccggataag acgcatcaaa cgtcgcatcc ggcgaaagtc aatcaggact cgctcaccac
    33661 caccgtaccg atttccgggt ggctaaagct ggtaatttta tccagacgta gctcacgggt
    33721 ttcgccagcg gcctcgacga ccagatactc cacatttttg cgggagacta aatcgctggc
    33781 ttttgcctgc aatttttcgc catctttcag ctcaagtgtc agcattaaat gatgctggca
    33841 ggcgagctca agattatcgt aatcatcaca attgattggt tgatacgtat cattcattga
    33901 cataatcgct caccagtaag tttgccgcag cgtatgctgc tttttccctg acagcctcag
    33961 aaagggcgtc gtcggcagcc atttcattca gcactttcaa aacgcagccc agcgcgtccg
    34021 gaacgtatcc taagtctccg ctggcgattt ccgcgtaccg cttgcgtatt aactcacaat
    34081 atttttccac atgccctcct gtcagcacac tgacttaacc gtggatgcaa gtctaagcct
    34141 acgaagataa actctgtttc gcaaggtgac tataccacac tcatttctgc aatatcagcg
    34201 ccgcaactgc acgtattccg ttacaatggc ctcctgattc gaaaggagtt ttcttatggc
    34261 gcttaaagcg acaatttata aagcgacggt taatgtggcc gatctcgacc gcaaccagtt
    34321 tctcgatgcc tctctgacgc tggcgcgcca cccttcagaa acccaggagc gtatgatgct
    34381 gcgcctgctg gcgtggctga aatatgctga tgaacgtctg caatttaccc gtggtttgtg
    34441 tgccgatgat gagccggaag cgtggctgcg taacgatcac ctgggcattg atttgtggat
    34501 tgagctgggg ctgccggatg agcggcggat taagaaagcc tgcacccagg ccgcagaagt
    34561 ggcgctgttt acctataata gtcgggcggt gcaaatctgg tggcagcaaa atcagagcaa
    34621 atgtgtgcag tttgccaatc tttccgtctg gtatctggac gatgaacaac tggcgaaagt
    34681 aagcgccttt gccgatcgta ccatgacgct gcaggcaacg attcaggatg gcgtgatctg
    34741 gttatcggac gataagaata atctggaagt taacttaacc gcctggcaac aaccttcatg
    34801 attgtgattt cccgacatgt tgctattccc gatggtgagc ttgagatcac cgccattcgt
    34861 gcgcagggcg cgggcgggca gcatgttaat aagacctcaa cggccattca tctgcgtttt
    34921 gacattcggg cgtccagcct gccagagtat tacaaagagc gtctgctcgc cgccagccat
    34981 catttgatca gcagtgatgg cgtgattgtc attaaggcac aggaataccg cagtcaggaa
    35041 ctgaaccgcg aagccgcgct agcccggctg gtggcagtga ttaaagattt aacaacagaa
    35101 caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag gctggcatag
    35161 aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg tcgggaataa
    35221 aaagaaggaa tagatggtga aaaaagcgat agtgacagcg atggctgtaa tcagcctctt
    35281 tactctgatg ggatgtaata accgggccga agtcgatacg ctttctccag cacaggctgc
    35341 cgaactgaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgctg attgtgaagg
    35401 aatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg agcgttatct
    35461 cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc gaaccgctga
    35521 caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga aaggcgatgc
    35581 gctggagatg ctcgatcgtg aaggcaatcc gattgaatcg cagttcaact atacgctgga
    35641 accggcacaa tccagtttgc ctatgacgcc gatgaccctg cgaggcatgt atttttatat
    35701 ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg tagcgaataa
    35761 cgcagagctg gagcgtggct acctggctgc gcgcggtcac agtgaaaaac cggtgttact
    35821 gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc caaccaaagt
    35881 attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta gtttggggca
    35941 gtaaacctgc ttaaggcaga aacaaacgca aaacaggcca gaaggatata tttcaacatt
    36001 ttgaatttgc acgttttttg taggccggat aaggcgttta cgccgcatcc ggcaatggtg
    36061 ctcaacgcct gatgcgacgc tggcgcgtct tatcatgcct acaacccccc tcatacctta
    36121 aggcggataa ggcatttact ttatcaccgg gtcaaaccaa caaagcgacc cgccttgata
    36181 taaatccctg gcttcaggtg cccaacatac tgtaactgcg tttctggctt aaacgcagag
    36241 acatcaccaa tacgcatatg catcagatcg gcagggctaa tccagcccga ttgcgccagt
    36301 gtaagtggat gtccggcttt ggcaaatgca tcagtgacaa attccgaaca aaaccacgac
    36361 tttttgtctc cttcgcccac actgcttaac tgcgctttcg ccaggccgct gacgcactgt
    36421 tggcgaaaat cctcggagaa cggattcagt gagcacatct ggcgagtcac cataaaggga
    36481 ataaattcga caatgccgcg atagttataa ccgctatctt tgattttatt ggcaaacgcg
    36541 gtgatatctg tggcttgttg cggggtaaga tccgggactc gtaagacgaa aagcttatca
    36601 ctatgcttca tcgctttttt aagggaaaca atatggacgc cagcgcctgt cgcttctgca
    36661 acgttattat cacccagaaa gattgcaatg tgactcacag aggaagtgct gaagacgcgg
    36721 attccaaatg aggttacccc aaggcttgag gagaacagca aatcgccggg tttgagatca
    36781 ggtgccgtta tttcttttat tgattgttcg gtgaaagagc tttgatgctg gaatttgaca
    36841 gcccatgttt ttgcctcggc atctactgct gtggctgatg ggtctggctg gctgatatca
    36901 acggtacaag ccgaaagtaa aagaaagctg gggagaagca ggcggcagta cgcctttggt
    36961 ttatccattt tatacaatcc atgtaaaaaa gggccctgaa attcaggccc ctttctggca
    37021 tcagccttta atctgtttca ccagatattc gacgatgtca ccagtcttaa ttaactgttt
    37081 ctcgccgttg cgacgatatt tatattcgat atcgtcgttg tcgaggttac ggtcgcccag
    37141 cacaatagtg tgcggaatac cgatcagttc catatcagca aacatcacgc ccggacgctc
    37201 tttgcggtca tccagcagca cttcgatacc ttgtgcacgc agttcgctgt acagtttctc
    37261 agcaagctct tgtacgcgga aggatttgtg catgttcatc ggcagaatcg ccacctggaa
    37321 cggcgcgata gcgtcaggcc atacgatgcc gcgttcgtcg tagttctgct caatcgccgc
    37381 agctaccaca cgcgttaccc cgataccgta gcaacccatc gtcaggattt ggttacggcc
    37441 atcttcaccc tgtacggagg ctttcagtgc ttcggagtac ttggtaccca gctggaagat
    37501 gtgaccaact tcgataccac gtttgatcag cagcgtaccc tggccatccg ggcttggatc
    37561 gccagccacc acgttacgga tatctgcaac ttccggggta gcgacatcgc gatcccagtt
    37621 gataccgaag tagtgtttac catcgatgtt agcaccagca gcgaaatcac tcatcgccgc
    37681 aacggtacgg tcaatcacca ccggaatcgg catgtttacc ggacccagtg aacccggacc
    37741 ggctttaacc acagcacgaa tttcttcttc ggtcgcgaaa gtcagcgggc ttgcaacctg
    37801 cggcagtttt tctgctttaa cttcgttcag ctcgtgatca ccgcgcacca gcagcgcaac
    37861 cagcgggaag ctgctgcctt caaccgcttt aaccagcaga gtcttaaccg ttttctcaat
    37921 cggcagattg aactgttcaa ccagttccgc gatggttttc gcgttcggcg tatcaaccag
    37981 cgtcatttcc tgggtagcag cagcgcgcgg ttctttcggc gcgatagctt ctgccagttc
    38041 aatgttcgct gcatagtcag aggtgtcgga gaagaccaca tcgtcttcac cgctctgcgc
    38101 cagcacctgg aattcgtgag aggcgctgcc gccgatagaa ccggtgtcgg cttgcacggc
    38161 gcggaaatcc agccccatgc ggctgaagat tttgctgtag gccgcataca ttgcatcgta
    38221 ggtttcctgc agggattcct gagaagtatg gaaagagtaa gcatctttca tcaggaattc
    38281 gcgggaacgc atgacgccga aacgcggacg cacttcgtcg cggaacttgg tctggatctg
    38341 atagaagttc agcggcagct gtttgtaaga gctaagctcg ttacgaatca ggtcagtgat
    38401 aacttcttca tgagttgggc cgagtacgaa cggacgctcg ccacggtcaa caaaacgcag
    38461 cagttccgga ccgtactgtt cccaacgacc actctcttgc cacaaatcgg ctggctgaac
    38521 caccggcatc gacacctcga ttgcaccggc gttgttcatc tcttcacgca cgatgttttc
    38581 gacttttttc agaacgcgca cgccggtcgg cagccaggta tataacccgg aggccagctt
    38641 gcggatcatc ccggcgcgca gcatcagctg atggctgatc acctcggcgt cggcaggtgt
    38701 ctccttgaga gtggagagca ggtattggct agtacgcatg ttgttacggt tccagttgga
    38761 aggtagaaca ggctcaaggc gagcctggga caaaaaaagt gatttagttt accagtgcaa
    38821 aagaaatgtc aaaagagaag ggcgtgaatt taacgcggtt ccagcgcaaa gacttcaaaa
    38881 cctgcgtcgg tgacgcgcca gcgaacgtta aaatcatgta gccagacggc ataggttttg
    38941 cccgtttcct cacctttatg ataggccggg cgcgggtcct gcgccagtac ttcgcggata
    39001 aacagcgtta actgcggata acgcttctcc agcgtcaaaa gctgcttttc gacctctgcg
    39061 gtaaaactca ccgccatctc tgcagctggc gcactttgcg catagctggc actggcatcg
    39121 ggaagcgatt cggcaaaggg gagatacggt ttgatatcca ctaccggcgt accatcgacc
    39181 agatccagac tgccgagctt cagaatcacg ctgtctttat ggcaaacaac ctctttcagc
    39241 tctaccagcg acataccaat tgggttaggg cggaaagtag agcgtgttgc gaaaaccccc
    39301 attctggcgt taccgccgag gcgcggtgga cgcacagtcg gacgccagcc gccttccatc
    39361 gtttgatgaa agacgaaaag gatccataaa tggctgaacg cttccaggcc gcgaacggcg
    39421 tcggcctggt tgtagggagc aatgagatgc agttcgccgt tggcgctttt taccagacct
    39481 ggctggcgcg gaacggcgaa tttttcttta tagggcgagc gaataacgcc tatttgctcg
    39541 aactggaaac tgctcatttc gccgtaatgt taagcgcaga gccgatacat acagcctgac
    39601 gatagcagcc tggcgtaccg ctggtgactt cgcagctatg cagtaatacc gcattggctt
    39661 tcattttaga ggcgttgatt tgcatccgct tacgcgcggt tggaatgctc ggcggagagt
    39721 cctgattaga ggcctggcaa gagtcgccac tgacttcacc gagatcgcgg aacggtttgc
    39781 cgactaattc ttctgcattg gtataaattc ggaccggcgt ggcgcgcggc gctttcggtt
    39841 ttgcaggctc cgctttcggc tggggtgcag tgctttgaac gggttcgaca ggggatctgc
    39901 ttaacatgga acagccgctt agcatgagtg ctactaaaca gatcggtaaa gcacgcatag
    39961 tatttcctca atgtatgatc aaaacgtcaa tattgaatca ggagcttgta aaaatgacaa
    40021 gacgggcaag tgcccatcct gaatgatatt acaaattgtg gaaacagcct aaaaattacc
    40081 agcctttaac agcgccgccg ttaaacactt tgtttgctgc ttcgtaaact tcatcagact
    40141 gataagcctg gacgaatttc ttcacgttct cggcgtcttt gttatcttca cgcgttacga
    40201 tcaggtttac gtacggggag tctttatctt cgacaaagat accgtctttc gctggagtca
    40261 ggccaatctg gctggcatag gtggtattga taactgccag agcgatttgc gcgtcgtcca
    40321 gagatcgagg cagctgcggt gcttccagct caacaatttt cagatttttt gggttctcaa
    40381 caacatcaag tacggtcggc agcaggccaa cgccatcttt cagtttgatc aagcccactt
    40441 tttgcagcag cagcagtgaa cgaccaaggt tagttggatc gtttggcacg gcaacctgcg
    40501 aaccatcctg cagttcatcc agtgatttga ttttcttgga gtaaccagca atcggataaa
    40561 caaaagtgtt gcctactgcg accagtttgt agccacgatc tttcagttgc tgatcaaggt
    40621 acggtttatg ctggaaggcg ttggcgtcga tatcgccttt gctcaatgct tcgtttggca
    40681 gaacatagtc gttgaaggtt accagctcaa cgtccaggcc atatttgtct ttcgcaactt
    40741 tctgcgcgac ttctgcaacc tgctgttcgg caccaacaat cacgccgact ttaatgtggt
    40801 ttggatcttt ttcatcctga ccgcagccta ccagtgccag tgatccaatc agggctccca
    40861 ctgccgcaaa ggttttgaat ttgaacgcca taccttattc cttcttcaat tatttatgtt
    40921 gtgttgaacg ttacttgcga gtgacagccc ggacgatgcg gtcgcctgcg aactgaatta
    40981 aataaaccag aatgaccagc aataccagta ccgtattcat caccgtcgcg ttatagccga
    41041 tgtagccata ctgatagcca atctgaccta aaccaccggc accgacagca ccacccatcg
    41101 cggaataacc gaccagggta atcagggtga tagttgccgc attcaccaga cccggcaatg
    41161 cttccggtaa cagcacttta cggacgatct gcatcggcgt ggcacccatt gcacgggaag
    41221 cttcaattaa cccggttggg atctccagca gagcgttctc gaccatacgg gcaataaacg
    41281 gtgctgcacc aacggttaac ggaacaatcg ctgcctgcaa accaatcgat gtaccaacaa
    41341 taacgcgggt aaacggaatc atccatacca gcaagataat gaacgggata gaacggaaaa
    41401 tgttcacaat cgcagaaacg gtacgataca gcttcgcgtt agcaataatt tgccccggac
    41461 gcgtgacata aagcagaacg ccaaccggca gaccaatcac aaagccaaaa aaaccggata
    41521 cgaaggtcat tgccagcgtt tcccatacgc cacgaaccag cagccacatc atcggctcag
    41581 acataaccca gtacctctac ttttacatgg tgttcctgca gccaggcaat ggcggcttgc
    41641 gtatcttgtt gtgtgccgtg catttcagtc agcatgatgc cgaacttaac gccaccggcg
    41701 tagtccatct gcgcgctaat aatgttgttg ttgacgttga aacgacgcgc ggtttcagaa
    41761 agcagtgggg catcgaccga ttgaccggta aactccagac gcagcatcgg cacgcagtca
    41821 gtaaatggct ccgcttgcag acgttcctgg taatcttccg ggatatccag atgcagggtc
    41881 gactgaataa acttctgcgc cagcggcgtt ttcggatgcg agaacacttc acttaccgtg
    41941 tcctgctcga tcagttcgcc attgctgatg accgccacgc aatcacaaat acgcttcaca
    42001 acgtccattt cgtgggtgat caacagaatc gtcaaaccca gacggcggtt aatgtctttc
    42061 agcagttcga gaatagaacg tgtcgttgcc gggtccagcg cgctggtggc ttcatcacac
    42121 agcaatactt tgggattgct ggctaatgca cgggcaatcg ccacacgttg tttctgccca
    42181 ccggaaagat tcgacgggta gctatcatgc ttatcgccaa gaccaaccaa tgacagcaat
    42241 tccgtcacgc gacgtttgat ctcgtctttc ggtgtgttgt ccagctccag cggcagagcc
    42301 acgttgccaa aaacagtacg cgaagagagc aggttaaaat gctggaaaat cataccaatc
    42361 tggcggcgag ctttggtcaa ctcggattct gacagcgtgg tcagttcctg gccatcgacc
    42421 agcacgctac cctcggttgg gcgctccagc aggtttacac aacgtataag cgtactctta
    42481 cccgcgcctg aggcaccgat aacgccataa atttgtccag ctggcacatg caggctgacg
    42541 ttgttcaacg cctggatggt gcgggtgccc tggtggaaca ctttggtgat attcgaaagt
    42601 tttatcattg attatttatt atcgtcatta agttagtcgt ggcatctcga atgcctgaaa
    42661 cgggcaacgc cgtcaatgaa atggatgtta aggcatccag acgtctaaat caatcaggtt
    42721 tatgcgaaga gcactttctt gcaggtcgaa acatgcgata ctagcgtcac atgccttatt
    42781 aaggagctat aaaaggtggc aaagagcgta cccgcaattt ttcttgaccg tgatggcacc
    42841 attaatgtcg atcacggcta tgtccatgag atcgacaact ttgaatttat cgacggtgtt
    42901 attgacgcca tgcgcgagct aaaaaaaatg ggctttgtgc tggtggtagt aaccaaccag
    42961 tctggcattg ctcgcggtaa atttaccgaa gcacagtttg aaacgctgac cgagtggatg
    43021 gactggtcgc tggcggaccg agatgtcgat ctggatggta tctattattg cccgcatcat
    43081 ccgcagggta gtgttgaaga gtttcgccag gtctgcgact gccgtaaacc acatccgggg
    43141 atgcttttgt cagcacgcga ttatttgcat attgatatgg ccgcttctta tatggtgggc
    43201 gataaattag aagatatgca ggcagcggct gcggcgaacg tgggaacaaa agtgctggtg
    43261 cgtacgggta aacctattac gcctgaagca gaaaacgcgg cggattgggt gttaaatagc
    43321 ctggcagacc tgccgcaagc gataaaaaag cagcaaaaac cggcataatg attaaaagat
    43381 gagcgattga aataaaaatg catttttcca cttgtcttcc tgagacgact ccctataatg
    43441 cgcctccatc gacacggcgg atgtgaatca cttcacacaa acagccggtt cggttgaaga
    43501 gaaaaatcct gaaattcagg gttgactctg aaagaggaaa gcgtaatata cgccacctcg
    43561 cgacagtgcg ctaaagcgcg ttgcaactgc tctttaacaa tttatcagac aatctgtgtg
    43621 ggc
//
LOCUS       DAJTNA010000010        42561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTNA010000010 DAJTNA010000000
VERSION     DAJTNA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42561
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.010"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..312
                     /locus_tag="OO161_000473"
     CDS             <1..312
                     /locus_tag="OO161_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5461656.1"
                     /translation="QAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVGAAAGSM
                     VNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPNTKCPEK
                     S"
     gene            328..867
                     /locus_tag="OO161_000474"
     CDS             328..867
                     /locus_tag="OO161_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5461657.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQVAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(962..2539)
                     /gene="guaA"
                     /locus_tag="OO161_000475"
     CDS             complement(962..2539)
                     /gene="guaA"
                     /locus_tag="OO161_000475"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5461658.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2608..4074)
                     /gene="guaB"
                     /locus_tag="OO161_000476"
     CDS             complement(2608..4074)
                     /gene="guaB"
                     /locus_tag="OO161_000476"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5461659.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGCLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4236..5606
                     /gene="xseA"
                     /locus_tag="OO161_000477"
     CDS             4236..5606
                     /gene="xseA"
                     /locus_tag="OO161_000477"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5461660.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5603..5818)
                     /locus_tag="OO161_000478"
     CDS             complement(5603..5818)
                     /locus_tag="OO161_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5461661.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGELIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5887..7359)
                     /gene="der"
                     /locus_tag="OO161_000479"
     CDS             complement(5887..7359)
                     /gene="der"
                     /locus_tag="OO161_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5461662.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQELSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7477..8655)
                     /gene="bamB"
                     /locus_tag="OO161_000480"
     CDS             complement(7477..8655)
                     /gene="bamB"
                     /locus_tag="OO161_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5461663.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8666..9286)
                     /gene="yfgM"
                     /locus_tag="OO161_000481"
     CDS             complement(8666..9286)
                     /gene="yfgM"
                     /locus_tag="OO161_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5461664.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9304..10578)
                     /gene="hisS"
                     /locus_tag="OO161_000482"
     CDS             complement(9304..10578)
                     /gene="hisS"
                     /locus_tag="OO161_000482"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5461665.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10689..11807)
                     /gene="ispG"
                     /locus_tag="OO161_000483"
                     /gene_synonym="gcpE"
     CDS             complement(10689..11807)
                     /gene="ispG"
                     /locus_tag="OO161_000483"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5461666.1"
                     /translation="MHNQAPIQRRKSTRIYVGYVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11834..12847)
                     /gene="rodZ"
                     /locus_tag="OO161_000484"
     CDS             complement(11834..12847)
                     /gene="rodZ"
                     /locus_tag="OO161_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5461667.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13132..14286)
                     /gene="trmG/rlmN"
                     /locus_tag="OO161_000485"
     CDS             complement(13132..14286)
                     /gene="trmG/rlmN"
                     /locus_tag="OO161_000485"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5461668.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14436..14867)
                     /gene="ndk"
                     /locus_tag="OO161_000486"
     CDS             complement(14436..14867)
                     /gene="ndk"
                     /locus_tag="OO161_000486"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5461669.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15016..17319)
                     /gene="pbpC"
                     /locus_tag="OO161_000487"
     CDS             complement(15016..17319)
                     /gene="pbpC"
                     /locus_tag="OO161_000487"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114026.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5461670.1"
                     /translation="MLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARVVVA
                     QDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARAAWQ
                     DLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNR
                     APFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAARNK
                     VLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAG
                     LQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMVNAI
                     RSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEALVR
                     SLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYT
                     AFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWK
                     TGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVDQFGFASAVPLLNQVNNILLSRSA
                     NLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEGING
                     IRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASESRAVRLPPASTSCPPYG
                     HDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTL
                     HLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17329..22289)
                     /gene="yfhM"
                     /locus_tag="OO161_000488"
                     /pseudo
     CDS             complement(17329..22289)
                     /gene="yfhM"
                     /locus_tag="OO161_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
     gene            22496..23341
                     /gene="sseA"
                     /locus_tag="OO161_000489"
     CDS             22496..23341
                     /gene="sseA"
                     /locus_tag="OO161_000489"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5461671.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(23834..24610)
                     /gene="sseB"
                     /locus_tag="OO161_000490"
     CDS             complement(23834..24610)
                     /gene="sseB"
                     /locus_tag="OO161_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5461672.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24752..26035)
                     /gene="pepB"
                     /locus_tag="OO161_000491"
     CDS             complement(24752..26035)
                     /gene="pepB"
                     /locus_tag="OO161_000491"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5461673.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26213..26413)
                     /gene="iscX"
                     /locus_tag="OO161_000492"
     CDS             complement(26213..26413)
                     /gene="iscX"
                     /locus_tag="OO161_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5461674.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26425..26760)
                     /gene="fdx"
                     /locus_tag="OO161_000493"
     CDS             complement(26425..26760)
                     /gene="fdx"
                     /locus_tag="OO161_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5461675.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26762..28612)
                     /gene="hscA"
                     /locus_tag="OO161_000494"
     CDS             complement(26762..28612)
                     /gene="hscA"
                     /locus_tag="OO161_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5461676.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGADVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28629..29144)
                     /gene="hscB"
                     /locus_tag="OO161_000495"
     CDS             complement(28629..29144)
                     /gene="hscB"
                     /locus_tag="OO161_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5461677.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTPFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29240..29563)
                     /gene="iscA"
                     /locus_tag="OO161_000496"
     CDS             complement(29240..29563)
                     /gene="iscA"
                     /locus_tag="OO161_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5461678.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29580..29966)
                     /gene="iscU"
                     /locus_tag="OO161_000497"
     CDS             complement(29580..29966)
                     /gene="iscU"
                     /locus_tag="OO161_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5461679.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(29994..31208)
                     /gene="iscS"
                     /locus_tag="OO161_000498"
     CDS             complement(29994..31208)
                     /gene="iscS"
                     /locus_tag="OO161_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5461680.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31320..31808)
                     /gene="iscR"
                     /locus_tag="OO161_000499"
     CDS             complement(31320..31808)
                     /gene="iscR"
                     /locus_tag="OO161_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5461681.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32078..32818)
                     /gene="trmJ"
                     /locus_tag="OO161_000500"
     CDS             complement(32078..32818)
                     /gene="trmJ"
                     /locus_tag="OO161_000500"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5461682.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            32937..33740
                     /gene="suhB"
                     /locus_tag="OO161_000501"
     CDS             32937..33740
                     /gene="suhB"
                     /locus_tag="OO161_000501"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5461683.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            33885..34739
                     /locus_tag="OO161_000502"
     CDS             33885..34739
                     /locus_tag="OO161_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5461684.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWLLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            34930..36225
                     /gene="csiE"
                     /locus_tag="OO161_000503"
                     /pseudo
     CDS             34930..36225
                     /gene="csiE"
                     /locus_tag="OO161_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
     gene            complement(36217..37356)
                     /locus_tag="OO161_000504"
     CDS             complement(36217..37356)
                     /locus_tag="OO161_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5461685.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37516..38406)
                     /gene="hcaR"
                     /locus_tag="OO161_000505"
     CDS             complement(37516..38406)
                     /gene="hcaR"
                     /locus_tag="OO161_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5461686.1"
                     /translation="MELRHLRYFVAVAQALNVTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKTVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASITA"
     gene            38542..39903
                     /locus_tag="OO161_000506"
     CDS             38542..39903
                     /locus_tag="OO161_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5461687.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYSETYAEAEQRLGEVCALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            39900..40418
                     /locus_tag="OO161_000507"
     CDS             39900..40418
                     /locus_tag="OO161_000507"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5461688.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40418..40738
                     /locus_tag="OO161_000508"
     CDS             40418..40738
                     /locus_tag="OO161_000508"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5461689.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40735..41547
                     /gene="hcaB"
                     /locus_tag="OO161_000509"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40735..41547
                     /gene="hcaB"
                     /locus_tag="OO161_000509"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5461690.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41557..>42561
                     /locus_tag="OO161_000510"
     CDS             41557..>42561
                     /locus_tag="OO161_000510"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR5461691.1"
                     /translation="MKEKTIIIVGGVQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLLPPPWFWSDQYSDNLQFIGDMRG
                     D"
BASE COUNT        10046 a        11757 c        10713 g        10045 t
ORIGIN      
        1 caagccttcg gcgcgcttat tggcgcagtc gctggcggtg ttatcggcca caacgtcggt
       61 tctggcagca attccggaac gacggcaggg gcagttggcg gcggagctgt aggcgcggca
      121 gcgggttcta tggtgaatga taaaacctta gtggaaggtg tttctttaac atataaggaa
      181 ggcaccaaag tgtatacctc tacacaggtg ggtaaagagt gccagtttac gacaggttta
      241 gccgttgtta ttaccacaac gtataacgaa acgcgtattc agccaaatac taaatgtcct
      301 gaaaagagct aataatcagg aggagtcatg aagaaagttt ttctttgcgc catcttggcc
      361 tccttaagct atccggctat cgcctcatca ttgcaggatc aactctctgc tgtagcagaa
      421 gcggaacagc aaggtaaaaa tgaagagcaa aggcagcatg acgaatgggt cgcggagcgc
      481 aacagggaaa tccagcaaga gaagcaacgt cgcgcaaatg cccaggtcgc cgctaacaaa
      541 agagcggcaa cggcagcagc aaataagaaa gctcgtcagg ataaactgga cgccgaagcc
      601 tctgcggaca aaaaacgcga tcaaagttat gaagatgagc tacgcagctt agagattcag
      661 aaacaaaaac tggcgctggc aaaagaagaa gcccgcgtta agcgagaaaa cgaatttatc
      721 gatcaggaac tgaagcacaa agctgcgcaa accgatgtgg tgcaatctga agctgacgcc
      781 aacagaaata tgactgaagg cggtcgcgat ctgatgaaaa gcgtgggcaa agcagaagag
      841 aacaaatcgg acagctggtt taattaatcg atgttagtaa cttcaagcct atgattcttg
      901 aagataaaaa accctctgta gtaacagagg gttttgttca ttcatagtgc agggattaaa
      961 atcattccca ctcaatggta gctggcggct tgccgctgat gtcatacacc acgcgggaaa
     1021 taccgttcac ttcattgata atgcggttgg aaacgcgacc gaggaaatcg tacggcagat
     1081 gcgcccagtg tgcggtcata aagtcgatgg tttcgacagc acgcagagag acaacccagt
     1141 catacttacg accatcgccc attacgccaa cggaacgtac cggcaggaac acagtgaacg
     1201 cctggctgac tttgttgtac aggtccgctt tacgcagttc ttcaatgaag atggcgtcag
     1261 cacggcgcag caggtcacag tactctttct tcacttcacc cagcacacga acgccaaggc
     1321 ctggtcccgg gaacgggtga cggtacagca tgtcgtacgg caggcccagc tccagaccaa
     1381 tcttacgcac ttcgtctttg aacagctctt tcagcggttc aaccaggccc atcttcatct
     1441 ctttcggcag gccgcccacg ttgtggtgag atttgatgac gtgtgcttta ccggttgcag
     1501 aagccgcaga ttcgataacg tcagggtaga tggtgccctg cgccagccac ttcacgtctt
     1561 ccagtttcag cgcttcttca tcgaacactt caacgaaaac gcgcccgatg attttacgtt
     1621 ttgcttccgg atcgttttcg ccagccagcg ctgacaggaa gcgatcttct gccggtacgt
     1681 gaacaatgtt cagaccaaag tgatcgccaa acatatccag aacctgctct gcttcgttga
     1741 gacgcagcag gccgttgtcg acgaatacgc aagtcaggtt tttaccgata gcgcggtgca
     1801 gcagcattgc ggttacggag gaatccacac caccagagag gccgaggatg actttatcgt
     1861 cgcctacctg ctcgcggatg cgagctacag catcgtcgat aattttcgct ggcgtccaca
     1921 gggcttcaca ctggcagata tcacgcacaa aacgctccag catgtgcata ccctggcggg
     1981 tgtgagtcac ttccgggtgg aactgtacgc catagaagcg tttttcttcg ttagccataa
     2041 tggcaaacgg gcagctttcg gtgctggcta cggtgacgaa gtcggacggg atagcggtaa
     2101 ctttatcgcc gtggctcatc cagacatcga gcagcggttt accgtctgcg gtcagcgcat
     2161 cttcgatacc gcgaaccagt gcgctgtcgt ttacgacttc aacctgcgcg tagccaaatt
     2221 cacgttcgtt agaggcttca acgtgaccgc ccaactgcat tgccatggtc tgcatgccat
     2281 agcaaacgcc gaataccggt acgcctgctt caaagacata ctgcggcgca cgcggactgt
     2341 tttcttcagt agtactttcc gggccgccgg aaagaataat gccgcttgga ttgaagtcac
     2401 gaatttgtgc ttctgtcaca tcccacgccc acagttcgca gtaaacaccc agctcacgca
     2461 cgcggcgcgc aaccagttga gtgtactgag aaccgaagtc cagaatgagg atgcgatgct
     2521 tatgaatgtt ttccgtcatt gacgcttatt ccgaggcaag tgaaacagat aatataaatc
     2581 gcccgacatg aagtcgggcg aagagaatca ggagcccaga cggtagttcg gggactcttt
     2641 agtaatggtc acgtcgtgaa cgtggctttc ctgaatgccc gcaccgctga tacgtacaaa
     2701 ctccgcttta gtacgcagtt cgtcgatagt accacagccg gtcaggccca tacaggagcg
     2761 caggccgccc atctgctggt gaatgatctc tttcaggcaa cctttatagg ctacgcgacc
     2821 ttcgatacct tccggcacca gtttgtcggc agcgttgtca ctctggaagt aacggtcaga
     2881 agaacctttg gacatcgcac ccagagagcc cataccacgg taagatttgt aagaacggcc
     2941 ctggtagagt tcaatttcac ccggagattc ttcagtaccc gccagcatgg aacccaccat
     3001 taccgcgctt gcgccagcgg cgatagcttt ggcgatgtcg ccggagaagc gaataccacc
     3061 atctgcgata accggaatac ctgtgccttc cagcgcttca actgcgtcag caacggcagt
     3121 gatctgcgga acacctacgc cagtaacgat acgagtcgta cagatagaac cagggccgat
     3181 accgacttta accgcgctgc aaccagcttc tgccaaagcg cgtgcacctg cagctgttgc
     3241 cacgttgccg ccgatgattt gcagatccgg atatttagca cgggtttcac ggatacgctg
     3301 caaaacgcct tctgagtggc cgtgggagga gtcgatcagc agaacgtcaa cgccagcggc
     3361 aaccagcgcg tcaacacgct cttcgttacc cgcacctgcg ccaactgcag caccaacacg
     3421 cagacggcct tgctcgtctt tacaggcgtt cggtttacgt tccgctttct ggaagtcttt
     3481 cacggtgatc atgccgatca ggtggaattc gtcatcaacc accagcgctt tttcaacgcg
     3541 tttttcgtgc atttttgcca gcaccacttc acgggcttca ccttcacgca cggtgaccag
     3601 acgctctttc ggcgtcatgt aaacgctaac cggctggttc aggtcggtaa caaaacgcac
     3661 gtcacgaccg gtgataatac ccaccagttc gttttcttcg gtaacaaccg gatagcccgc
     3721 aaaaccgtta cgctcggtca gttctttcac ttcgcgcagc gtcgtggttg gcagaacagt
     3781 ctgcggatca gtcaccacac cagattcgtg ttttttcaca cggcgaactt cttctgcctg
     3841 gcgttcaatg gacatgtttt tgtggataaa gccgataccg ccttcctgag ccagagcaat
     3901 agccaagcgc gcttccgtta cggtatccat tgctgcggaa agcataggga tattcagacg
     3961 aatagttttc gtcagctggg tgctgaggtc agcagtattc ggcagaacgg tagaatgagc
     4021 aggaacgagg agaacgtcgt caaacgtcag agcttcttta gcgatacgta gcatgggcaa
     4081 tatctcgacc agagtggtta ataaatattg ccgcggcatt atacagagcg taaccgattg
     4141 catctacccc tttttgcaaa aaatgcttgc tatccccgaa gggcgggtta ctatcgactg
     4201 aataacctgc tgatttagaa tttgatctcg ctcacatgtt accttctcaa tcccctgcaa
     4261 tttttaccgt tagtcgcctg aatcaaacgg ttcgtctgct gcttgagcat gagatgggac
     4321 aggtttggat cagcggcgaa atctctaatt tcacacaacc ggcttccggt cactggtact
     4381 ttacactcaa agacgacacc gcccaggtac gctgcgcgat gttccgcaac agcaaccgcc
     4441 gggtgacctt ccgtccacag catggacaac aagttttagt tcgcgccaat attacgctct
     4501 acgagccgcg cggtgactac cagataatcg ttgagagtat gcagccggcc ggtgaagggc
     4561 tgctgcaaca gaagtacgaa cagctcaaag cgaagttgca ggctgaaggt ttgttcgatc
     4621 agcaatacaa aaaaccactt ccctcccctg cgcattgcgt tggtgtgatc acctcaaaaa
     4681 ccggtgctgc gctacatgat attttgcatg tgttaaaacg tcgcgatcct tctctaccgg
     4741 tgatcatcta ccccaccgcc gttcagggcg atgacgcacc ggggcaaatt gttcgcgcca
     4801 ttgaactggc gaatcagcgc aatgagtgcg acgtgttgat cgttgggcgc ggcggcggtt
     4861 cgctggaaga tttatggagt tttaacgacg aacgcgtagc gcgggcgatt tttgccagcc
     4921 gcattccggt cgtcagcgcc gtcgggcatg agacggatgt gaccattgcc gattttgttg
     4981 ccgatctgcg tgcgccaacg ccgtctgccg ccgctgaagt agtgagccgt aatcagcaag
     5041 agttactgcg ccaggtgcaa tcgacccgtc aacggctgga gatggcgatg gattattatc
     5101 tcgccaaccg cacgcgtcgc tttacgcaga tccatcaccg attacagcaa cagcatccgc
     5161 agctccggct ggcacgccag caaaccatgc ttgagcgcct gcaaaagcgg atgagctttg
     5221 cgctggaaaa tcaacttaag cgtaccgggc aacagcagca gcggttaaca cagcggctga
     5281 atcagcaaaa tccacagccg aagattcatc gcgcgcaaac gcgcattcag caactggaat
     5341 atcgtttagc agaaaccctg cgcgtacagc ttagcgccac gcgtgaacgt ttcggtaatg
     5401 cagtaacgca cctcgaagcc gtaagcccac tgtcaacgct ggcgcgtgga tacagcgtta
     5461 ctactgctac tgacggcaat gtactgaaaa aagtgaagca agttaaagcg ggtgaaatgc
     5521 taaccacacg tctggaagac ggctggatag aaagtgaagt taaaaacatc cagccagtaa
     5581 aaaaatcgcg taaaaaggtg cattaagcca gcacgaattc aacgcgtttt ttcgatatca
     5641 agccatgccc gtgctgacag aagtaatcta ctgcaccgca ggcttttaac acctgcaacg
     5701 gttgatggca gtctggacaa agagctttca tttcaataag ttccccgcag gatgggcaac
     5761 gcgcatggcc gttatcctga tcaagcacat gctgacattg tggacaatgc agttccatat
     5821 gaattcctcc ggtagccatc cctctacatt catagaggga atggcagata aaatacttac
     5881 ggataattat ttatttttct tgatgtgctt catcagacgc ttacgtttac gcatctgggt
     5941 tggcgtcagg gtgttacgct tgttcgcata cgggttttcc ccttctttga actgaatacg
     6001 aatcggcgat cccattacgt ccagcgattt gcggaagtag ttcatcaagt agcgcttgta
     6061 ggaatcaggc aggtctttca cctgattacc gtgaatcacc acaatcggcg ggttataacc
     6121 accagcgtgg gcatatttca gcttcacacg acgaccgcgt accagcggcg gttggtgatc
     6181 ttcaacagcc atcgtcatga tgcgcgtcag catagaggtc cccacacgac gggtggagct
     6241 gtcatacgct tcacgtactg attcaaacaa gttaccaaca ccactgccgt gtaaggcaga
     6301 gataaagtgc acacgagcaa aatcgataaa gcccagacgg aagtccagcg tttctttcac
     6361 ctgctctttc acttcctggc tcaggccatc ccacttattc accacaatga caagtgagcg
     6421 cccactattg agaataaagc ccagcagcga gagctcctga tcggaaatac cttcgcgcgc
     6481 atcaatcact aacatcacca cgttggcatc ttcaatggcc tgcaacgttt tgattacgga
     6541 gaatttctct acagcatcgg tgattttgcc gcgtttacgt acgccagcgg tgtcaatgag
     6601 cacatactca cgtccatcgc gttccattgg aatgtagata ctgtcacgcg tcgtgccagg
     6661 catgtcataa acaacaacgc gctcttcacc aagaatacgg ttagtgagtg tagacttacc
     6721 tacgttcgga cgacccacaa tcgccagttt gatcggcaga ctttgcgggt cgaagtcgtc
     6781 ttcctcttct tcttcgccgt tctcttccgc ttcaaattgc gcccagtatt cagcgtcttc
     6841 gtcgacttcc tcttgcggtg cgagatcttc catccacggc agcagcacat gctccagcag
     6901 acttaatacg ccacgaccgt gagacgcggc gatcgggtag atttcaccta agccaagcga
     6961 gtagaaatca accactgcct gatcgggatc cagaccgtca gttttgtttg ccaccaagaa
     7021 ggtcggtttt tcacgggagc gcagatgttt ggcaatcgct tcatctgccg gcatcaggcc
     7081 cgcgcgcgca tccaccataa acagtacgac gtccgcttct tcaatcgcca gcagcgactg
     7141 ttccgccatg cgggtttcta caccgtcttc tgtgccatca atcccgccgg tatcaataca
     7201 gataaactca cggccttcaa tttccgcacg accgtactta cggtcacgag tcagacccgg
     7261 gaaatccgca accagcgcat ctcgggtgcg agttagacgg ttaaataacg tggattttcc
     7321 tacgttaggg cgcccgacaa gcgcgaccac aggtaccatg tttaaagcct catttttata
     7381 aatcatcaga caacgcacgc tatattcgcg tcgttgttaa aaacaggaaa acggcccctg
     7441 tccaggagcc gttttcaaag tgaacgacag agacgattaa cgtgtaatag agtacacggt
     7501 tccgtctttt gcctggatca gcagtttgcc gtcagcggca accggttcag tctggaaacc
     7561 ggaactatca actttttgct gggcaacgaa acgaccatct tcgacattaa tccagtgcag
     7621 ataaccttca ctgtcaccga ccaccaggtt gccatcatac agcaccggag aagtcagcag
     7681 gcgatgcaac agatcgcttt gtgtccacag cgtaacgccg ccatcaatgg tcaacgccat
     7741 cacccggtca ttttgatcga ccagatagat gcgattgccg tcgacgatga aatcattcac
     7801 cgaacccagt tcgcgtttcc acataatctg accactgcgc agatcaagcg ccgtcaggtt
     7861 accattatag gccagcgcga aaacaacgcc gttaacaacg acgggagtcg tgtcaacatc
     7921 gctcagacgg tcaatttcgg tagaaccggt cgcctgggaa atacgctgct gccaaatcat
     7981 ctggccctgt tccatcagca ctgcgctgac gcgaccatta tcgcccccca cgacggccgc
     8041 accaaaagcc gttgccggcg cagactcgcc acgcaaagag agcgaaggca tatcgaggtt
     8101 aactgtccat ttgacagcgc cgtcagcttc gttcagcgct tgtaactgac cgttactggt
     8161 gtggattaac accagaccgt cgctgaccac cgggcgcgaa agtgcttcac ccgcgacttt
     8221 agtttgccat gccacagtac catcgctggt attcagcgcg taaacctgcg ccttttcgct
     8281 gccaatgtag acatgcccac cagacacggt cacaccgcca gaaagtaatg caggctcttt
     8341 agagaaccag ccatctttct cggccaggct gacagaccag atttctttgc catcatccgc
     8401 attcagcgct tttaccaaac cagcgcggtc cgctgcatag acaacgttgt ccgccagtgc
     8461 cggatgaaga ttggaataga agttgccaat gccgctacca acggaagtac tccacgccgt
     8521 ggtcggcgta aactggtttt caacggttgg caatggggac atctttacca catcttcttc
     8581 gctgttaaac agcgaacagc cgcttaaaag ggtaacggaa agcagtcctg gcagcagtaa
     8641 tttacgcaat tgcatcgggt ccctctcaga tggacaaatt attaattttc atctgcatca
     8701 tttcgctcag tgccggagta acatcgcttt tcacgcctgc ttcccatgca ctacgcgcac
     8761 cttgcttatc acctttgctc agcaatgctt caccacgcag gtcggcaaca atggcagccc
     8821 acccttcacc tttgatggta tcaagggttt tcagcgcggc atcagcctgc ttgagctgta
     8881 cctgaacgcg agcaagacgc agatttatca cggctttgag attttcatcg ctcgtgtctg
     8941 ccagcccctg ttgtaactgg gcggcagctt tctctagttc atttttgtca acaaattgct
     9001 gcgcaagttc caaagaagcc agcgcaccat aagtattttt attttcagca gcaaattttt
     9061 ccgccgccgg gatgctatcc ggtttgcctt cgctcactgc ggtaaccgca ttttgatagg
     9121 caagagaagc ggagcgtgca gaatcaacct gatggctgtt ccagtagcgc cagccaatca
     9181 gtgcgccaac gcccaaaatc accccaacag ccagtgcttt gccattttca gcaaaaaagc
     9241 gtttaaccgc ttctacctgg tcgttttcgt tctcgtaaat ttccacgctg tccttctcct
     9301 tccttaaccc agtaacgtgc gcaaatgcgc ggctacgcta tcctgcgcaa ctgccgtttg
     9361 ctcaccagag cgcaaatcct tcactactgc tgtgccgtta gtcacttcag actcacccag
     9421 caccacagca acgcgggcac cccatttatc agcacgggca aactgtttct taaagttgcc
     9481 gccgccgtgg ttggtcatca atttcacgcc ctgtaattca tcacgcagac gctcagctaa
     9541 tgccatagcc gcagattgtg tatcagcacc tgaagccacc aggtatatat cgacaatagg
     9601 atcggcttta aattccggat taacggcctg tactaacaat acaagacgtt cgaggcccat
     9661 cgcaaaaccg acagccggtg ttgcacgacc gcccagttgt tccacaagac cgtcataacg
     9721 accgcctgca cacacggtgc cctgggagcc gagactgtta gtcacccact cgaaaacggt
     9781 acggttatag taatccagac cacgcaccag acgctggttt acggtgtaag cgatccccgc
     9841 gctctcaagc agtttgcaca gaccggcaaa atgctcacga gattcctcgt ccagataatc
     9901 acctaatgcc ggagcgtcgt tgagaagcgc ctgcacttcc ggattttttg aatccagcac
     9961 gcgcagcggg ttagtgtaca tgcggcgttt gcagtcttcg tccagctttt ctttatgctg
    10021 ctcaaggaat gccaccagcg catcgcggta attggcgcgt gcttccagcg aaccgataga
    10081 attcagctca agagttacgt gttcggaaat acccagcgcg cgccaccagc gggcggtgag
    10141 cataatcagt tcagcgtcga tatccggacc ttgcagaccg aaaacttcgc agcccaactg
    10201 atggaactga cgataacgcc ctttctgcgg acgctcgtga cggaacatcg gcccgatata
    10261 ccacagacgc tgttcctgat tgtacagaag accatgctcg atgccggcgc gtacacagcc
    10321 cgccgtccct tcagggcgca gagtcaggct gtcgccattg cgatcctcaa aggtgtacat
    10381 ctctttttca accacgtcgg tgacttcacc aatcgcacgt ttgaatagcg gggtctgctc
    10441 tacaatcggc aagcggattt cactgtaacc gtagctgccg agcacgtttt tcagtgtgcc
    10501 ttcaatgcgc tgccagatgg ccgtttcgcc aggcaggtaa tcgttcatgc cgcgaatggc
    10561 ttgaatgttt tttgccacgt ttattctctt tctgaatata aaaatgaacc ctcaacgctt
    10621 ccctcaatgt ttggggagcc atgcgggttc aatcatacac gggaagcgag gcgcttccca
    10681 tcacgttatt atttttcaac ctgctgaacg tcaattcgac gcgcttcatc cagctgactg
    10741 gctttcgcac gaatgcgtgc ttccagctgg tcgatcatat cgttgttgtc cagacggtct
    10801 ttgcgcacgc catcttcata gaggccgctt ttcttgttgc cgccggtgac gccgagtgta
    10861 gaaaccagcg cctcacctgg gccattcacc acgcagccga taatcgaaac gtccatcgga
    10921 gtgatgatat cttccaggcg ttgctccagc gcgttaaccg taccgataac atcaaattcc
    10981 tgacgcgaac aggtcgggca ggcgatgaag ttgatccctc gcgaacggat acgcagcgat
    11041 ttcaaaatat cgaaaccgac tttgatctct tcgaccggat cggccgccag cgatacgcgc
    11101 agcgtgtcgc cgatgccttc agacagcagc agacctaaac caatggcgga ttttactgcc
    11161 ccgctgcgtg caccaccggc ttcggtgatc cccagatgca gcggctgatc gatctgtttt
    11221 gccagcaaac gataagactc aacagcgagg aagacgtcag acgctttcac gctgactttg
    11281 aactgatcga agttcaggcg atcgagatga tcaacatgac gcatggcgga ttccagcaac
    11341 gcctgcggcg tcggttcgcc atacttttct tgcagatctt tttccagcga tccggcgtta
    11401 acgccaatac ggatcggaat gtttttatcg cgcgcacagt caaccaccat gcgaatacgc
    11461 tcttcattac cgatattgcc agggttaata cgcagacaat cgacgccgta ttccgctact
    11521 ttcagcgcaa tgcgatagtc gaagtggatg tcagccacca gcggcacgtt aacccgctgt
    11581 ttgatgagtt tgaacgcttc tgccgcgtcc atcgtcggta cggagacacg gacgatatca
    11641 gcgccaacgc gttccagcgc cttgatttga ttgaccgttg cttcgacatc tgtcgtacgc
    11701 gtattggtca tggactgtac ggcgatggga gcaccatcgc caatcggcac atacccaacg
    11761 taaatacgtg ttgattttct acgttgaatt ggagcctggt tatgcatgaa aaatctcccg
    11821 cgttacccgt ctgttactgc gccggtgatt gttcggcatt gagggtcaga cgcgcaacct
    11881 ggttagttct gataaaacga ctcagatcga caggtttccc ttgatactgg atctgtactg
    11941 cggctggcgc accaattttc agtttgtacg gtgcctggcc ggttaagttc aaattaccgt
    12001 ctttacgctg cataccgcta aacaattttt taccggtagc atcagtgacc tccagccagc
    12061 aatcggcagt aaagttcatc accagcgcat tcggatcagc caccggcgtg gtcacgccag
    12121 cctgatcggt tggcaacggc gcagcaccat ctggcgttgt tgctgccgtt ggtgccgggg
    12181 tcgcggcggt atcaacattt gcctgcgaag gcgaaacaac cgcattctgt tgcggatcaa
    12241 cagctggtgc tggcgcagtt acggcaggtg tttgcgtgtt ggttgcggta gtatccacag
    12301 acgctggcgg cgtggacgtt gtagccgggt ctgtagttgt cgacgtattt aacggaacac
    12361 tctgcccctg ctcgctatta ctgctcagtt ccgccgaaga ttgatcggcc atagtggtga
    12421 tctcttcctg ctgagctttg tggtcttgcc accaccaggc accgctcagg ccgataacca
    12481 caaacaacac cagccaggtg aaggtcatca gccagccgtc acgttttttg cggcgtttac
    12541 cgagggaaaa actctgcatc ggcgcaactt tcgctgcccg tagtggagcc tgcttttcca
    12601 gccctggcag cagttcttct tctggaatat gtaccagacg cgcataagag cggatatatc
    12661 cgcgcaggaa tgttgaagca agatcggcgg gtgccttatc ttcttcaatg tcgcgtaccg
    12721 tggaaacctt caggcaaagt cgctcggcaa cggcctgctg actaagtcct agttgttcgc
    12781 gagcattacg caggcgagcg ccggtagtaa gtgcttcatt ttggtcgtgc gtggcttcag
    12841 tattcattcg ctgcaggtac gtttaaatga gaattgagat gccggtgaac catcatgctc
    12901 acccacaccg cgaaacatcc gttaagttaa ccgttatcat acagtataag accgtctgtg
    12961 tgctcgtgac aaagcccaca ttcactaagg ctaacttact gttgcatcgt tacatactgc
    13021 cttaaagtca gcaaaaacgc accgttaata ttgaccagac aattgcaact taatacttca
    13081 ttcataaaag attacgccac ggtacataaa gtaaccgtgg cgtaatggct atcagaccgc
    13141 tttaatgtcg atggcttcac cctgcatccg tttacgcagg gtacgtttcg tacggtcgat
    13201 aacatcgccc gccaactgac cacaggcagc atcgatatca tcaccacgag ttttacgcac
    13261 aatagtggtg aaaccgtagc tcatcagcac ttttgagaaa cggtcgatac ggctgttcga
    13321 actgcgtcca tacggcgcgc ccgggaacgg gttccacggg atcaggttga tcttacacgg
    13381 cgtatctttc agcagctccg ccagttggtg cgcgtgttca gtgccgtcgt taacgtggtc
    13441 aagcatcacg tattcaatag tgactcggcc ctgattggcg ttggatttct ccagataacg
    13501 gcgcaccgca gcaaggaacg tttcgatatt gtactttttg ttgatcggca caatttcgtc
    13561 acgaatttcg tcgttcggcg cgtgcaggga aattgccagt gcaacgtcga tcatatcgcc
    13621 cagtttatcc agcgccggaa ctacaccgga agtagaaagc gtgacgcgac gtttagacag
    13681 gccaaaaccg aaatcatcaa gcatgatttc catcgccgga acgacgttgt tcaggttgag
    13741 cagcggctcg cccatgccca tcatcactac gttagtgatt gggcgctgac cggtgacttt
    13801 tgctgcgccg acgattttcg ccgcacgcca cacctggccg ataatttccg acacccgcag
    13861 gttgcggtta aagccctgct gggcggtgga acagaattta cactccagcg cacaccccac
    13921 ctgagaagag acgcagagcg tggcacggtc gtcttccggg atatacaccg tttcgacgcg
    13981 ctgatcgcca acggcgatcg cccatttaat ggtgccgtcg gatgaacgct gttcttcaac
    14041 cacttccggt gcgcggattt ccgccacctc tttcagtttg ccgcgcaaca ctttgttgat
    14101 gtcggtcatc tcatcaaagt tgtcgcagca atagtgatac atccacttca tcacctgatc
    14161 ggcgcggaag ggtttttcac ctaaatcttt aaaaaactcc cgcatctgct gacggttgag
    14221 atccagcagg ttgatttttc catctttcgt ggtgacgttt tcaggtgtga ctaattgttc
    14281 agacatatgc tattccggcc tcgttattac acgttatggc ccctggaggg ttgaaaaaag
    14341 aaacgccccg gtgagcggct gctcgtccgg gggcgctgca ttgtacaaat tctggcgcac
    14401 ggatgccacg tttgcacgcg gcatttacga aattattaac gggtgcgcgg gcacacttcg
    14461 ccttcgccaa agaaataagc gatttcgcga gcggcagatt cgacggaatc agaaccgtgg
    14521 gtaccgtttt cggtcaggct gtcagcgtaa tcagcgcgca gagtaccagc cagtgcgttt
    14581 gccggattgg tcgcgcccag cagatcgcgg tgacgctgaa cggcgttttc accttccagc
    14641 acggaaacca cgatcgggcc agaggtcatg aattcaacca gaccatcaaa gaacggtttt
    14701 ccatcgtgtt cagcataaaa gccacgtgcc tgttcaacgg tcaggtgcag cattttggtg
    14761 ccgacaattt tgaaccctgc agcttcaaag cgcgcaaaga tattaccaat gacgtttttt
    14821 gctaccgcgt tcggtttgat gatggaaaaa gtacgttcaa tagccatttt tacctctgta
    14881 aattgttctg ttgttgtctg taccagcgta cgagatggcg cggattataa tgagcaacag
    14941 ggccgttgac tattgatgaa ggtaacattt ttttaaaata aaatgagttt tagcaacaat
    15001 cagggacaaa acagactatt gcatgacaaa tttcactgtc gcgatttgcc ccacgtcatc
    15061 catcaccagc aattgataat cacctttatc cgtcaaatgc agggtcacgt tgcgcccgcg
    15121 ttcagttaac ggttcgccat tcagaaacca ccagcgttca cctgcccctc cacttgattg
    15181 caacggcaaa gtcgcttccg ctgcgcccgg taaacgttta ataatcgcgc catcgcgaac
    15241 gcctgtcagc tgcagcggga gctgggcatc gtgaccgtat ggcgggcaac ttgtcgaggc
    15301 cggtggtaag cgcacagcgc gactttctga tgcgggcagc cagggttcca gcggtagcgg
    15361 ccagacattt atcatttctt gtcgcgcttg cgggcaatca gcggcaacac gtttgccatt
    15421 ttcatccagc cagatgggga aacgaatgcc attaatgcct tcctgctccg gcagtaatag
    15481 agttggcggc tgactcccgt ccagcagcca ggttgccagg cggcggcgac agttaccgtc
    15541 accttccggc aaagactgtc cgcccggcca gcagataacg ccacgagtga ctgagttcgg
    15601 gcgcgggtct tccggcagat tcgcactgcg cgacagtaag atattattga cctgattcaa
    15661 caatggtacg gcactggcaa agccaaactg atcaacaacg ggcgtgccgt ccggtctgcc
    15721 agtccagatc ccaatgacat agcgagcgtt aaccccaatc gcccaggcgt cacgatagcc
    15781 atagctggtg cccgttttcc atgccagtgg ggcgacgcgc ggcaaggcac tatccggcaa
    15841 gggttgcgct tcatcagcca taatccggcg aatgatccac gccgcccccg acgacattaa
    15901 aggccgttca agcagcggat cgtcaggctg taagcgcaat ttgcctgcct tgccgtggcg
    15961 agcaaacgcg gtatacgctg ccgccatatc ttccagtttt gcaccagcgc cgccgagaat
    16021 gagtgaaaga ttcggcgcag caccgttggg caaatataac ggcaatccaa cattgcgtaa
    16081 ctttgccgca aaccgtttcg gtccataggc ttccagcacc tgcacagcag gtaagttcag
    16141 cgagcgcacc agcgcctcgc tcatgctgat cgggccatga aaaccgctat caaagttacc
    16201 tggtcgataa tcaccggtgc gccgggggac gtcttgcagc agtgatgccg ggtggatcaa
    16261 gccttcatcc agcgccagac cataaacaaa cggtttgagc accgatcctg gcgatcggat
    16321 cgcattgacc atatcaacat gaccaaagcg tgaatcatcg ttgagatcaa ccgatcccac
    16381 ccagccgcga acacgcatat cggtatgatc aaccacgatc atcgccagtg agctgcgcgg
    16441 tggcaaccgc cctttccagt tttgcgccag ttcttccaga cgtcgttgaa gaccggcatc
    16501 cagcgtagta acgattttgt cgcttttgct tttaccgagc atcatgcgcg aaaacagcgg
    16561 tgccagttgc ggcatttgtc ggggtgccag ccagatgggt tcttcccttg actcttttac
    16621 ctgctcacgg gaccacacac cttgcacggc catccgttcg agcactttat tacgcgcggc
    16681 ttcggcacgc tccggccaac gatccgggcg cagtcggctg ggcgcttgcg gtaacaccgc
    16741 cagcattgcc gcctcggaat agcttaaatt cgcaggcgat tttccgagat aagcccaact
    16801 tgccgcaccg atcccctgca acgtaccgcc aaacggagcg cggttaagat acaagatcag
    16861 aatttcacgc ttagacaaat gccactccag ttgcaacgcg cgccagagct ggagaatttt
    16921 gccaccaaat gttttggggt gaggatcaag cagacgagca acctgcatag tgagcgtgct
    16981 gccaccagaa ataacccgtc ccgaagtgag atcttgccat gctgcgcgcg ccaccgagaa
    17041 tggattcacc cccggatgct tccagaacca gcgatcttca taattgatca gcgcttcaag
    17101 gtaacgtgga gacacatctt cgattgttac cggataacgc cagatgccat cagcatcggc
    17161 gaagcgccag agcggcgtac catcctgcgc cacgaccact cgtgcgggat tgacttcatg
    17221 cagcggtaga ggccagagtt tatctgctcc ccacgctgca agaaaaagga gaaatggcac
    17281 agctgccagc gttatccagc agccgcgttt ggttaacaag cgaagcgttt acggtcggac
    17341 aatcagcaga tcttcagccg cgccggtcgc ccgccattgg ggaacataca ttgattccac
    17401 catcggttgc ggtacctgat acgttcccgg cgtcaccgcc cgcgccagat acaccagcgt
    17461 taccggttgg tattcatcaa cggcaaccgc cgccacaaag cgatcgtcac ggaactcaat
    17521 gtgctaatgc tcgcctgctg catctggttc agtaagtttt gcacttcgcc accactttgc
    17581 tccaggctgg cgctaccgtt cgccagattc tgattttcca gttccagacc cgcaggcagc
    17641 agatccacga ctaacgcatc cggcacgctg ttactggctt ttacctgcaa ccagaccagc
    17701 accagatcgc cgctacgtaa cgagtccagc gatttgctct taccatcagt accaagaata
    17761 tgacgctcga tttgcagcac attgttcgcc ggtaaaggtg cggattgcgg ataaccgctg
    17821 gcatccatac gcaaccataa cggctgatcg ccactgtttc tcacctgcaa ggtggcaagt
    17881 tgatcgctat tcagattgct gtttagcgtt ttctcgcctg tcagcggctc agctgagaaa
    17941 gaggtttgcg cctgccattt acccggtaaa tcctgaatcg tacgggcagc caggaacaac
    18001 gcgttacttt cctgcgtcga tagccagcgt tcaccaaacg cctgctgcga aagtgtgttc
    18061 agcaaagtgt actgctcatc gggtagcagt ttattttctt ccagcaagga gagcattaac
    18121 gcgttgtcgc gcagtggact accgtaatca cccagccata tccgctcatc actatttctc
    18181 ggcgttttca gcgccagcac aatcgcttct tcaccacgcg ttgcatcacc catggttttc
    18241 agcgcaatgc caagttgcag cagcggtaaa ccagaagcgg catctgcacg atgctcccag
    18301 atttcacgca gcgcacccag tggtgctttt tgctgacggg ccagcaccag cgcagcgtaa
    18361 gactgaacgg cgaatttact ggctttgaga ttatccgcgt acgggatcga catcatgccc
    18421 ggatcttgta aatagcgcag cagacgctca ttaccccggt taatggcgtc tgtcggcacg
    18481 ctgtaaccct gttcgcctgc gcggaccagg aaatccatca cgtaagccgt cagccagtac
    18541 tcttcgtcac cgtttttatc ccacagcgca aagccgccgt tatcacgttg catttgcagc
    18601 aaacgggaaa tgccgatatc gaccgatgca cggcgtttct catcactgtc gcctttgatg
    18661 cccaacgcct gcagttgggc tgcgttggta taaagtgacg ggaacaggcc gctggcggtt
    18721 tgctcaagac agccgtacgg atacgctttt aactctttga tataacgtgc aatgttcagc
    18781 ggtggtttgc cgctcaacaa caattgccct tccagcgtaa caggcgagaa gttttgcaat
    18841 ccatccgccg gaatcgccca tgtctcacca agctgtaacg ccgtaccgta attaaccgtt
    18901 tgtgccggga acgccggacg gacgccgatt ttccactgct tatgctgatc ggcaacggtt
    18961 tcacccggta acgctaaccc gctaatggtg gcctgaattt ctccatcgcc ataacccggc
    19021 aatgctcgca ccgggataaa cagcgtagta cgcacacctg gcgctaattc aacggctgcg
    19081 ggtgaatcgc tgaccagttc aagcaaacca ctggcggtca gggcaacgtt cagtttttgc
    19141 ggtttatcgg taagattagt gatatccagc gtcagacgcg aggtatcgcc actcgccata
    19201 aagcgcggca tgttcagttc agcaatcacc ggtgcggcaa ctatcacttt gctttcgttg
    19261 ctaccgaaat catctgccgt ccaggcttgc gccatgacgc gcaattcacc gttaaaatcg
    19321 ccaatcggca gtgtaaccga gccttcgccc tgttcgttga gcgttaccgg cagcgcctgc
    19381 tgcgcgacaa tattgacgtg attgaccggc ggtttaccac cacgtttcag ctcatcacca
    19441 tcgccaccga aacgcagagc tgccagacgc ccctgacctt caataacctg accgtaaata
    19501 tcgtaaatgt ctgcgccatt gcgtttctga ccaaagaacg cctgccacgg atctggcgtg
    19561 acgtagtcag taatattcag cacaccacta tcaacggcag acaccagcac attcacctgt
    19621 ttaggcttct cgccattttt agtgctggct ttaattttca cggttaatgg ttgattggga
    19681 cgcatttttg ctggtgtttc cagcgccaga tcgaggcgac ggttttcatc gccaagcggc
    19741 agatgcaaca caccaaccgc gcgttttggc gtcgcggagc gagatttatc gccaggacgt
    19801 accaccagcg tacttaaata cagatcatga cgattccagg ttttatcgac cggaatcgtc
    19861 agatccagcc cttgagccgg aacatcaatc tcttgccacc acagcggccc ttcactggac
    19921 tcgaccatcg cataaccttt acccgccgtt ggcgcggcga tatgcaactt aatggtgtcg
    19981 ccagggcgat aactggcttt atccagtttc agcgtgacac ggtcgggtcg cactgcgccg
    20041 ctaccgtcgc tgttgtcctg ccagctatag ccagcccaga aacgaacact actgaccgct
    20101 tcattcggcg ctttgacttc cagacgataa gcgccccact ctaccggaaa actgaccttg
    20161 ccggtttcgt ccgctttcag atccagagtt tgttcatttt cgatcagatc tttttgatca
    20221 aactgtgact gccagccttc atcttctgac cagttccagt agtaatcgcg gcgttcgcga
    20281 atcaggcgca cctgcaagcc cgacacggct tttttcacgc cttgtgcatc gctataaacg
    20341 atgtcaaaag cggcgttact gccttcatca acaatgggct gtttcaccgt gctgtcagtg
    20401 cgataatcgt aaaccgattt caaggcgaac tgcggacgga gccccggcaa tgcatcggca
    20461 ggccagatag cctgctcagc gcggcgcgtc accgggcgac cgcccgattc cagcaaactg
    20521 ccctggaaaa taacctgcaa tggggaatgt gtttccttcc actggctttc tgtagaaact
    20581 tcgccgcgcc ctttatcatc cagcgtcaac tgaacttcat ccagcgtgcg ggaaaggttt
    20641 tcggcagcta tatcgccgaa ttcaaaacca ggtaaggctg acacagcctc acgcagtggg
    20701 cgcaggaaaa gttgcccttg caaagtatta ccattagcag gcgcaccgta caggtagtat
    20761 cccaccacgg agaatttcac ttcatcattc ggcgtaagcg gggttttctc accggtcaga
    20821 ttcagcgcca tacgctctgg cataaaatct tcgacgtgga aatcccacat ccggtactgg
    20881 ttatcgcccg tgttggcgcg aatatgccac ataccggttg ccgcattgct atcgagtggc
    20941 caggtaaagt ggtagaggcc attctccggc tgactaacga cgctcctgag tacctgcccg
    21001 tcaggtttaa tcacgtctaa cttgatgggt tgatcgggca atgctttacc gtctgcatcg
    21061 cgcagtaaac cattgaggat taccgtttca cctgggcgat aaagatcgcg tgggccaaac
    21121 atgaaaaact gtttgctata gcctggcgcg ccagcaatgt taaattctgc taagtccagc
    21181 gccggaagtt ttaaatcgag tagcgttgtc tgaccgtctt tacgtgccag cagtaatgcc
    21241 gcatttttat cattttccag ctgcacatgc ccctgagcgt cactggttgc ctgagtcaga
    21301 gtctgccctt tctcatttaa taaagagact tcaattcctt gctgggccgc gccgttttcc
    21361 agactttggg taaagatatc cagacgattg tgataacggt gagctgaaac gccgatatca
    21421 cttaacgtaa acagcgtcgc gggattactg taatcgtaac gtccagcctg attcatcaca
    21481 gccagataca cgcccgcctg ctgaagcggt ttgatatcgc ccagcggcag caataatttt
    21541 tcacgggtgt tacgcgcagg attgagatca aaccgtccgg tgtagaccag atccgccatc
    21601 tgcagcagtt tgtctgactg ccagttcgcc agcgaattgc ggtattccca ttggctaatg
    21661 aatgctggca gagattctgg cttaacgcgg aagaagttaa catcgacatt attaacgttg
    21721 agcgccatta ccggcagccc ttcaacgact ttgccaggca gcagcgaacc acggctggca
    21781 aaaccgacgc tgggttggat gtcgcgggta gttatagttt tttcgtaatc tttactgaag
    21841 gttgcgttgt tgagtgcttt gacctccttg ccaatagtaa cgatcaaatc acgtttcggt
    21901 tcgaggtggc gtaaacgtag ctctttaaga ttatctgaca gctcccaggc accatccact
    21961 ttgccgcttt ttttatcgac gacatgaata acgcgtgaga aatcctgatc cgggtcgaga
    22021 gggatggaga acgtcagcac cagcgtggct gcaccatcaa gttggacttc agagagatcc
    22081 agcaatgtca gcaccttacc ggcactctgt tgggccagtt tttgtctctc cggtacggag
    22141 agttttgctg aactcgcgtt ttctgaagag gccgctttag taacttcaga aggcgcatct
    22201 tttttcaccg ctgttggcgc gttatcgttg ttgtcgcacc ctgccagcgc cagcattagc
    22261 atgcaggcgg ctacgcgtaa ctttttcatt tttcatccct gccacaatgg cccgttagca
    22321 acgtcgaata attattatgc gtgagaattt acgttatgta attcagtatc acgcccatac
    22381 aatccgaatt ttagtgagaa ttgtggctca gacagccaaa tcagcctctt tcgcttgtcg
    22441 cttttcacta aacatccgac aatttagcct aaccccggca aaaatggaga tgcctatgtc
    22501 cacgacatgg tttgtaggag ccgactggct cgccgaacat attgatgacc cggaaattca
    22561 gattatcgat gcccgcatgg cgtcgcctgg acaggaagat cgtaacgttg ctcaggagta
    22621 tctgaatgga catattcccg gcgcagtgtt ttttgatatc gaagcgcttt ctgatcacac
    22681 ttccccgctt ccgcacatgc tgccgcgccc ggaaacgttc gccgtggcga tgcgtgaatt
    22741 aggcgttaac caggataagc acctgattgt ctatgacgaa ggtaatcttt tctcagcccc
    22801 acgagcatgg tggatgctgc gcacctttgg tgtagagaaa gtgtcgattc tggggggtgg
    22861 acttgcaggc tggcagcgcg atgatctgct gttagaagaa ggtgcagtag agctgccgga
    22921 aggagagttt aacgccgcgt ttaatcctga agccgtggtg aaagtaaccg atgtattatt
    22981 ggcaagccat gaaaatacgg cgcaaattat tgatgcccgc ccggctgcac gttttaacgc
    23041 agaagttgat gaacctcgcc caggtttacg tcgcggacat attcccggag cactgaatgt
    23101 tccgtggacg gaactggtgc gcgaaggcga actaaaaacg accgatgaac tggatgcgat
    23161 attttttggt cgcggcgtca gctacgacaa accaattatc gtcagctgcg gctctggtgt
    23221 aacggcagcc gtggttttgt tagcactcgc gacgctggat gtgccaaacg tgaaactgta
    23281 cgacggcgca tggagtgaat ggggcgcgcg ggcagattta ccggttgagc cagtgaaata
    23341 agtattttac aggcaataaa aaaccgccga atttggcggt tttttattgc tggtctggtt
    23401 cgcggccttt ccagcaggtt gtattaccgt agtaatgcaa gcgcgtctca gcggagacaa
    23461 tactcgccag taactctctt tttgtcaagc aaaagagagt tattattgtt ctgttagtat
    23521 attatccact gcggcccttt ccgccgtctc gcaagcgggc gctggcttaa ggaaaggatg
    23581 ttccgcgacc gttagagcaa gcgcgtctca gcgcttgcta tcgcggcaat atcgccagtg
    23641 gtgctgtcgt gatgcggtct tcgcatggac cgcacaatga agatacggtg cttctgtatc
    23701 gtacttattg tttctggtgc gctgttttcc caagtaaata ataaccggag tctctccggc
    23761 gacaatttac tggtggttaa caaccttcag agcagcaagt aagcccgaat gccgcccttt
    23821 agggcggcat attttagatt atccggtttt gtttaaagtc acgcaaaaaa cctccccagc
    23881 gacgttcata gaatggcaca atatgttcgg taataaagtg gctaattcct ttttcccctt
    23941 ttttcacctg acaaatatcg attggttcat cgccaggtaa tgtatcggtc gctacacttc
    24001 ccgtcgcctg aataatttct tcgatatcac catcggcttc aatgccaata aataaattag
    24061 gctgtgcctc ttcgttctct ttaattgaac aaataaaagc acgcttcacc ggcttaatgg
    24121 ttttaaataa ggtggtgagt gaatcaatca tttgtgctgg cggctctgcg acttccgata
    24181 atatcagcga ttcaccgcct tccaggattt cctggctgct cagcggattt ccctcttcac
    24241 caatcaacaa actgatttca cgcggcataa attctttacc ggtcggcagt ttggcattaa
    24301 ggaagagcgt ttcgccaagg gtcatctcaa acaatgtgcg aacgggcatt acgacaaatg
    24361 cctgttcgtc ttcaaccgcc tgttgaagcg cttctaacga ggtgaaaaaa ggaatgacgc
    24421 tggtgccgtc ttctttttcc cagtgctgta aatcaagcgc actatcttca accacagcct
    24481 cgccctgcgc cgccgtacca ggcacccaga cggtggattc cagtagagta cggaaaaagg
    24541 ccgggcggtg cgccggttcg gttgctgctt tttccagcag gtcttcaagt tcgttttttg
    24601 tttcggacat aagaaccaca attcattcaa cgttcggggc gcaaatgcct gatgcgctac
    24661 gcttatcagg cctacaaggg attcgcaatt tgttgaattt gcagaatttg taggccggat
    24721 aaggcgttta cgccgcatcc ggcatattag tttacgccgt taacagatta gctatcgtgc
    24781 gcacaccaag tcccgtagcg cccgcagacc actgttcaac cggcgcttta cggtaagtcg
    24841 ccgagcagtc gatatgcagc cagccttgct gatagttctc aacaaagtgc gacaggaagc
    24901 ccgccgccgt gctcgcgcct gccggatacg ccgcgcttcc ggtattgttc agttcggcaa
    24961 agttagacgg cagctggctg cggtggaatt ccgccagcgg cagacgccag aatggttcgt
    25021 tctcttgtga ggcactcgcc agcaaacgac cggcaagcgc atcgtcaaaa ctgaacagcg
    25081 cgtgataatc attacccagc gcagttttcg ccgccccggt gagggtcgcc gcatcaatga
    25141 tcatttccgg tttctgcgca ctggcatcaa tcagaccatc ggcaagcacc agacgccctt
    25201 cggcatcagt gttcatcact tcaacttttt taccgttgcg ataggtgatg atatcgccca
    25261 gcttgaacgc attgccgcta atcaggttat ccgcacagca gaggaacagc ttcacgcgct
    25321 tgttcagtcc gcgcgtaatg gcaaatgcca gcgccccggt aaccgttgcc gcgccgccca
    25381 tgtccgactt catcgagtcc ataaacgcag tctgtttgat gctgtagccg ccggagtcaa
    25441 aagtgatacc tttacctacc aggcacgcgt acactggcgc ttctttatcg ccagttgggt
    25501 tgtaatccag cgccagcaat accggagaac gttctgaagc gcgtccaacg gtgtgcagcc
    25561 ccatataacc ttgctcacgc agatcttcgc ctttggtgat ccgataagtc acacgatcgc
    25621 ccgcgacgtt gctgatcaga tcaacagcac gctgtgccag ttgcgatggt cccaattctt
    25681 ctgccggtgc gttgatggtg tcacgcaccc agtcgatgat catcaggcgg ttatccagtt
    25741 cctggcgctg ggcatcgtcc agatccggcc actccacttt acgcgtgcct ttcggggctt
    25801 tgtaaccttg ccagaatgcc cagcagcgat ccgcatccca gccttcaccg cttaactgaa
    25861 catgcttgat gcccagaccg tcaatcttgc gcgccgcacg ctggatcagc cccagatcgt
    25921 ctgccccgtt caaatgcagg gtaatgccgt cattattaat gctgtaagtt gctttttctc
    25981 cccagcgcgc gtcggcaggt tgggtagaga gggtaatctt catcgcttct gtcattttag
    26041 ttatccttct ttgtaaaagg gccgccagtt ggcagcccca ggatttttcc gctaaatgat
    26101 tcgcgttgca ggaaggcggc aagtgagtga agccccagga gcatagataa ctatgtgact
    26161 ggggtgaacg agcgcagcca acgcatctgc ggcgtgaagc atgacgcgga aattattcgg
    26221 cctcgtccag ccagactaac aaaatcgctt cgaggatttt ctcgttggat gcctgcgggt
    26281 cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat atcggtgaat cgaaccgttt
    26341 tcggatcaag atcaggatac gcatcgtaca gtgcttcgcc aatttcgcgg ctatcggtcc
    26401 acttaagtcc catactaacc tctgttaatg ctcacgcgca tggttgatag tgtaacgcgg
    26461 gatttcgact actaaatctt cgtcggtaac gcgtgcctgg cagcttaaac ggctttcggg
    26521 ctccagtccc caggctttgt ccagcatgtc gtcttcctgc tctgagcttt ctggcagcga
    26581 gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa gcacaggatt tttcacaggc
    26641 gtgttcaatc tcgataccgt tacgcagcgc tgcgtcgaga atggtttcac cgctattagc
    26701 ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc aaaataacaa tctttggcat
    26761 attaaacctc gtccacggaa tggcctttca gcgcacgacg aaccgactgg tccatgcggc
    26821 gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat cgcttgttcg atggcgtcaa
    26881 catcagcgcc ctgagccact tcactcaggt gagcggcagc attgtcaatg acctgacgct
    26941 ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc gtgcagactt tccagcacac
    27001 gcgccgcttc tactttttgt tctgccagca tacgggcttt tacgtcctgc tcggcatagc
    27061 tcattgaatc tttgatcatc gaagcgattt cgctatcggt cagaccgtaa gaaggtttga
    27121 cctgaataga cgcctcaacg ccggtggatt tctccatcgc cgtcacgctc aaaagaccgt
    27181 cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc agccggtagc gccggaatac
    27241 cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac cagttcgcgc tcaccctgca
    27301 ttacatggat agacatcgcc gtctgaccat ctttaaaggt ggtgaaatcc tgagcgcggg
    27361 ccaccggaat agtggtatta cgcggaatca ctttctccac caggccgccc atcgtttcaa
    27421 ggcccagcga cagtgggatc acatcaagca gcagcatttc gctgtccggc ttgttaccca
    27481 ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac tttatccggg tcgatggaag
    27541 tcagcggtgg acgaccgaaa aattcgccta cccgttcacg caccagcggc acgcgagtag
    27601 aaccgccaac catcaccact tccagcactt catcagcttc tacgcccgcg tctttcagcg
    27661 cacgacgaca agccagtaag gttcgtttta ccagtggcgc gatcagttca ttgaattgtt
    27721 cacggctgat ttcgccctgc cagcccgcaa cgttaacggt cacggagtcc gcatcgctca
    27781 gcgcgatttt ggctgcaatg gcggcatcca gcagttcacg ctgaacgcgg ttatcgctac
    27841 gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag cagatggtcg aaatcatcgc
    27901 cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa cacgccgcga cttaagcgca
    27961 gaatggaaat atcaaacgtc ccgccaccga ggtcataaac ggcgatcacg ccttcctgac
    28021 cggaatccag cccgtaggcg atagccgcag cggtcggttc gttaagtaag cgcagaacat
    28081 gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg ctgggcatcg tcaaagtacg
    28141 ccggaacggt gataacgaca ccatccagct cgcctgccag ggcttcagtt gcccgcgccg
    28201 ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt cagcagcccc gccgccgttt
    28261 caatcattgg caggccgttt tcgctggcct ggaattgata aggcaaatgc ggatagcgtt
    28321 gctggatatc agccagcgag cgtcccatca ggcgtttaac agaactaatg gtgttggcgg
    28381 tatcgagcgc tgcgttagta cgcgcgtcat aacccaccga atgcccttgc tgttgatagt
    28441 gaacaacaga tggcagcagg tgacggcctt catggtcggc taacgtttcg gcctgaccgc
    28501 tgcgcactgt cgctaccagc gagttggttg tgcccaggtc aataccggcc gccagacgac
    28561 gctgatgcgg cgcagcactc aaaccaggtt cactaatttg taataaggcc atgtttagct
    28621 tccagaaatt aaaaatcgag cagtttttct tcgagttgtt cggcactgct tcgcagttta
    28681 tcgagaaaac gcagcttacg cacggtatcc gccgccgcgt cccacgtctc gttgtctaac
    28741 tgttcaacca tcaactgatg gcgggtatca aacatctttt tcacacgttt gataaagctt
    28801 tccagccgcg cttcatcttt cgcctgttcg atctcgtcca gttcttcgcg cagctccagc
    28861 tgttccatca ggaacggggt gtcgcgcaca gtatgctgct cgctggcgag atcaaaaccg
    28921 tgcaaagaaa gcaaatattc cgcgcgcatt aacggatgac gcagcgtttg ccaggcctgg
    28981 ttaatggttg cagattgctg tacggcggcg agttgttccg cctggcttcc gctggcgaat
    29041 ttatcaggat gatactgacg ttgtagatcc tgaaaacgca ggctcagtgc ctgggtatcg
    29101 agttgataac gggcaggcaa gccaaagagg gtgaagtaat ccataacatt ctcagggctg
    29161 cgatccgccg cgataacggc gaatcaggta aaacaaaccc cacgcgcagg cgaccacggt
    29221 ggggttatcg gtatgcgcat caaacgtgga agctttcgcc gcaaccacac tcatctttaa
    29281 cgttcgggtt ggtgaattta aacccttcat tcaggccttc ttttacgaag tccagctgcg
    29341 taccgtccag aaattgcagg cttttgccat cgaccacgac tttcacgcct ttgtcttcaa
    29401 acacgatgtc ttccggcgtc ggttcgtcaa caaattccag tacataagcc atacctgaac
    29461 atccggaggt tctcacgccc agacgcaggc caaacccttt gccgcggtta gccagaaagg
    29521 tatttactcg cgctgctgca ctgtcgctca gtgtaatcga cataaccaaa cctcaactct
    29581 tattttgctt cacgtttgct tttatagtcc gcaatggcgg ctttgatcgc gtcttctgcc
    29641 agaatagaac agtgaatttt caccggcggc agttcaagtt cttcagcaat atcggtgttt
    29701 ttgatcgcct gcgcttcgac gagagacttc cctttcaccc attcggtgac cagggagctg
    29761 gaagcgatag cagaaccgca gccgtaagtt ttaaaacgcg cgtcttcaat gataccttca
    29821 tcgttgactt taatctgcaa cttcatcacg tcgccacagg ccggtgcacc caccatgccg
    29881 ctgccgacgt tctcgtcgtt gttgtcaaag gaacccacgt tacgcggatt ctcgtaatgg
    29941 tcgataactt tttcgctgta agccattata aattctcctg attccgatac cgattaatga
    30001 tgagcccatt cgatgctgtt cagatccacg ccctgcttgt acatttccca cagcggagaa
    30061 aggtcacgca gacgaccgat ggatttacga actaactcga tggtgtagtc gatctcttct
    30121 tcagtagtaa aacgacctaa agagaaacgg atagagctat gtgccagctc gtcgttcagc
    30181 cccagcgcgc gcagcacgta ggacggttcg aggcttgctg acgtacaggc ggaacctgaa
    30241 gaaactgcga ggtctttcag cgccataatc agcgactcac cttcaacgta gttgaagctg
    30301 acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt tcaggtaaac ttcttcgata
    30361 tctttgatgc cgttccacag acggttacgc aggccgcgca gacgttccat ctcggtcgcc
    30421 atctcttctt ttgcgatgcg ataggcctcg cccattccga cgatctggtg aacaggcaga
    30481 gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca tttgcgcttc gatgcgtacg
    30541 cgcggtttac gacgtacata cagcgcaccg atacctttcg ggccatagat tttgtgaccg
    30601 gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga taggcagttt gcccacgctc
    30661 tgggttgcat caacgtgata gataatgcca cgagcacggc acatttcgcc gatagccgcg
    30721 atatcctgca ccacgccgat ttcgttattt acgtgcatga tggaaacaag gatggtgtcg
    30781 tcacgcatcg ctgcttcaag ttctttcagg tcgataatgc cgttacgctg cggtgccagg
    30841 taggtgactt caaaaccttc gcgctccagc tgacggcagg tatccagtac cgctttgtgt
    30901 tcggttttgc tagtgatgat gtgcttgcct tttttctgat aaaagttggc tgcacctttg
    30961 atcgccaggt tgtcagattc ggttgcacca gaggtaaaga cgatttcacg cggatcagcg
    31021 ccgaccagat cggcaatctg attacgggcg atatctaccg cttcttcagc ctgccagccg
    31081 aaacggtgag aacgagaggc cgggttacca aaggttccgt ccatcgtcat gaactgcatc
    31141 attttctcgg caacacgcgg gtccaccggc gtggttgcgg agtagtcgag ataaatcggt
    31201 aatttcattg ctctataaac tccgtacatc actcaatgca aggaatcagg ctagcggctg
    31261 gatgtacgac cgtgtttacg gagtatttag cactccggcc tgattctgaa ttctttttat
    31321 taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg tgcgtggcgc gtcgtgagta
    31381 tgctgacgac cagacacatc cagcacttcc tggttattaa ccagttcgcc taaagtaatg
    31441 ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc acagcgcgtg ggtcaggcat
    31501 ttatcgccgc cctggcagcc gcctttaccc tgacaacggg tggcatctac agattcgtca
    31561 acggcgctaa tcacttcgcc aacggcgatg ctgctggcat ctttgcctaa cagataacca
    31621 ccgcctggtc cacgtacgct ggaaaccaga ccatttttac gcagacggga aaacagttgt
    31681 tccagataag aaagggaaat tccctgacgt tcggaaatat cagccaacgg taccgggccc
    31741 gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg catagcgccc tttagatgtc
    31801 agtctcatgt cttacttcac ctcaaactcg cccctgccca gggtttttta ttgtaaaagt
    31861 gggggtattg aatagcaggg ccaagtctga cattcccgag taaattggtc aactatttac
    31921 ttgactgatt tagtcgggta tttaaccttc agtgccattt ttttatcgtg gcgtttgtac
    31981 ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg gttctgtaca ttgtaggcct
    32041 gataagacgc gttaagcgtc gcatcaggca actactgtta ttcggccttg ttacctttat
    32101 tctgctgctc aatagaagcc agaatcccgc gcaggatatt caattcctgg ctttccgggc
    32161 gcgcacgggt aaacagacgg cgcaatttat tcatcacctg ccccggatgg ttttcacgga
    32221 taaaaccggt tgccagcagc gtttgttcca gatgaccata aaaacgctcc agatcatcga
    32281 ccagcggata cggcgtctct tcatgctcga cctgctcgcc gttttcctga gttgccagcc
    32341 aggccatgcg cacttcatag gcaataacct gaaccgccat cgccaggttc agcgagctgt
    32401 attccgggtt agccgcaatc gcgacatgat aatggcattt ctgcaactct tcattggtca
    32461 agccgacgcg ctcgcgacca aacaccagcg ccaccggggt atttgccgct tcagcgacgc
    32521 ttttcaggcc gcattcgcgc gggtcgagca tcggccatgg cagcgtgcgg gaacgtgcgc
    32581 tggtgcccac caccaagcta caaccggcta acgcttcgtc gagcgtatcg acgatgtgag
    32641 cattaccaat cacatcgctg gcccctgctg ccagggcaat cgcctgggag tcgggtttca
    32701 ccagtggatt aaccagccac agattggtta atcccattgt tttcatggca cgggcaacag
    32761 aacccatatt gccggtgtgt gacgtctcca ccagcacaat tcgaatattt tgcagcattg
    32821 tctttcttca tctaaagatt attcgcgcat cttatcataa aacgaagaca gatgccgatc
    32881 tcgctgctat actctgcgcc gttttcccgt tctttaacat ccagtgagag agaccgatgc
    32941 atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc gggtaattta attgccaaaa
    33001 actatgaaac cccggacgct gtagaagcga gccagaaagg cagtaacgat ttcgtgacca
    33061 acgtagataa agctgccgaa gcggtgatta tcgacacgat tcgtaaatct tacccacagc
    33121 acaccatcat caccgaagaa agcggtgaac ttgaaggtac tgatcaggat gttcaatggg
    33181 ttatcgatcc actggatggt actaccaact ttatcaaacg tctgccgcac ttcgcggtat
    33241 ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt ggtatacgat cctatgcgta
    33301 acgaactgtt caccgccact cgcggtcagg gcgcacagct gaacggctac cgtctgcgcg
    33361 gtagcaccgc tcgcgatctc gacggtacca ttctggcgac cggcttcccg ttcaaagcaa
    33421 aacagtacgc cactacctac atcaacatcg tcggcaaact gttcaacgaa tgtgcagact
    33481 tccgtcgtac cggttctgcg gcgctggatc tggcttacgt cgctgcgggt cgtgttgacg
    33541 gtttctttga aatcggtctg cgtccgtggg atttcgcggc aggcgagctg ctggttcgtg
    33601 aagcgggcgg catcgtcagc gacttcaccg gtggtcataa ctatatgctg accggtaaca
    33661 tcgttgctgg taacccgcgt gttgtgaaag ccatgctggc gaacatgcgt gacgagttaa
    33721 gcgacgctct gaagcgttaa tgactcaggc gggtgatgtc actcacccgc cctcgccttt
    33781 caggcgctat tccgaaatac ttcctcaccg ctttactttc tttcgtcact ctcccaccat
    33841 ctttccccga ttaatggatg ataaagaagt cacaaggaaa ggttatggcg ctgccagtga
    33901 acaaacgcgt tctcaaaatt ctgtttattc tctttgttgt tgccttctgc gtttatttag
    33961 tgccgcgcgt tgccatcaac ttcttctatt atcctgacga taaaatttac ggtcccgacc
    34021 cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg tactcgtctg caaggctggt
    34081 ttattccttc ttcgacgggc cctgctgaca atgccatcgc aaccatcatt catgctcacg
    34141 gcaatgccgg aaatatgtcc gcccactggc tgctggtcag ttggttaccc gagcgtaatt
    34201 tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc aaaaggcacg ccgtcccagg
    34261 ccggattgct ggacgatacg caaagtgcca tcaatgtggt gcgccatcgt agtgatgtaa
    34321 acccacaacg gctggtgctg ttcgggcaga gtattggcgg ggcgaatatt cttgctgtta
    34381 ttggtcagga tgatcgtgaa ggcatacgtg cggtgatcct cgactccaca tttgcctctt
    34441 atgcaaccat cgccaatcag atgatccccg gcagtggcta cttacttgat gagagttaca
    34501 gcggcgaaaa ttatatcgcc agcgtcagcc cgatcccgct tttactcatt cacggtaaag
    34561 ctgatcacgt tatcccatgg cagcacagcg aaaagttgta tagcctggca aaagagacaa
    34621 aacggttgat cctaatcccg gatggcgaac acattgatgc tttttccgat cgtcacggcg
    34681 atgtttatcg cgaacagatg gtggacttta tccttagcgc gttgaatccg cagaactaac
    34741 ccatgatcgc tagcacgata atcattcaca aaaccacctt aagacatgct aatccactgg
    34801 tcagaacagt ttaagatgag aaaaattctg tgacgcttgc caacatttct gatgattagc
    34861 attcccttcg ccatttccct gagcaaactt tagctattct tatcaattat gcttatggga
    34921 gatctacaga tgatgcctac gcttgctcca ccatctgtcc tttcggctcc ccagcgccgc
    34981 tgccagatct tgctgacgct ctttcagccg gggttgaccg ccaccatggc aaccttcagc
    35041 gagcttaatg gtgtggatga tgatattgcc agtcttgata tcagcgaaac aggacgggag
    35101 atcctgcgct atcatcaact cacactgacg actggttatg acggtagcta ccgggttgaa
    35161 ggtacagtgc ttaaccaacg tttgtgttta tttcactggc tacgacgtgg tttccgtctg
    35221 tgtccgtcat ttattaccag ccaattcacc cccgccctga agagtgaact gaagcggcgc
    35281 ggaattgcgc gtaactttta cgacgatacc aatctacaag cgttagtgaa tctctgctcc
    35341 cgacggctgc aaaaacgctt tgaaacgcgc gatattcatt tcctgtgtct gtatctgcaa
    35401 tattgtttgc ggcaacacca cgctggaatt acgcctcagt ttaatccgct ccaacgtcgc
    35461 tgggcggaat cctgccttga atttcaggta gcgcaggaaa ttggacgcca ctggcagcgt
    35521 cgggcgctcc agcctgtacc gcctgatgag ccactgttta tggcattact tttttccatg
    35581 ttgcgggttc ccgatccatt gcgtgatgcg catcagcgag acagacaatt gcgtcagtct
    35641 atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg ttcgttttta tgatgaacag
    35701 gggttatgcg atcagcttta tacccacctc gcccaggcgt taaatcgcag tttgtttgcc
    35761 atcggtattg ataataccct gccagaagag ttcgccagac tgtacccacg cctagtgcgc
    35821 accacccgcg cggcgctggc cggatttgaa agtgaatacg gcgtccatct ttctgatgag
    35881 gaaagtggtc tggtcgcggt gattttcggt gcctagctaa tgcaggaaaa cgatctgcat
    35941 gaaaaacaga ttattcttct taccgggaat gatagcgagc gagaagcgca gattgagcag
    36001 cagttacgcg aactaacgtt actgccgctc aacattaaac atatgtcggt aaaggcattt
    36061 ttgcagacag gcgctccgcg cggcgcggcg cggcgcggcg cggcactgat tattgcgcct
    36121 tataccatgc cgttaccgct cttttcacca ccgctgatct atacggacct gacactgaca
    36181 acacatcaac aggagcagat ccgcaaaatg ctcgaatcag catgagggaa caactttcgg
    36241 gcgcaaaaac attgccggaa gcgccaccag cgccattacc cagaacacgc cgtggcccag
    36301 atattgatac aggaaaccgg cgaaaacggt catgatagct atactgccgc ccatcgcgac
    36361 ggcagagtaa accgcctgta aacggatgac ttcgctaccc tggcgagcgg caatatagcg
    36421 catggcggcc aggtggcaga ccgtgaaggt gccgcaatgc agaatttgca ccactatcaa
    36481 ccacggcaac gccgtagttg ctcccataat gccccagcgc actacgccgc aaatcgccga
    36541 gatcaacagc atatcgcgtg cactacaacg gcggaaaagt ttattactca gcgcaaagat
    36601 aatgacttcc gccaccacgc ccagcgacca caaatacccc accgccgagg ccgagtagcc
    36661 agctgcctgc cagtaaatgg cgctaaaacc gtaataggcc gcatgtgccc cctgcaataa
    36721 acaaacgcag gccagaaagc gccagttctg aagaaccagc gccaaccacg ctgaccaacc
    36781 ggtgctctcc tgctggcggc ttgccccttg tggctgaatc gtcggacgga tgagaaagcc
    36841 gagcagcatg gatgccacgc ccaatgttaa cagcgcgagg atcacctgat aatcaaacat
    36901 agtgaccagt ttgcccgtca gcgccgagcc aatgacaaac gccaccgagc cccacagtcg
    36961 cactttgccg taatcaagcg ggaactgctt ttgccacgta ttcgccagtg catcggtcaa
    37021 cggtaccagc ggtgagaaaa agaggttaaa gccaatcatc accagcatca gccacgctac
    37081 gtgcgccccc gcccagaagg cgacagcaaa gagaagtgtc atcattgcca gcacgcgcaa
    37141 ggcggaaatc aggcgagaag gatcgctgac gcggggcgcg atgagcaaac tcccgagaaa
    37201 acgggcaacc agacctgccc ccaataacag gccgatggtt tctggcgtta aaccaatccc
    37261 tttaagccag acgctccaga aaggtagaaa aatgccataa ctaaaaaagt atgtgaaata
    37321 gccgagcgcc aaccagcgcg tggattgcaa aaccatgagt ccctcccgtc aggaggcgtt
    37381 agtctggggt taatcgaatg ttggtgcaag ttgaaaaata agaatattcg agcctgcttc
    37441 ccgatgtctg taacgccagt tcacaagcac taaaaaactg cgatccgcga cgcatgttgc
    37501 aaaaataatg cgtaattatg ccgttatgct tgccaaacgt tcctgcacaa tggcgatgaa
    37561 atcgcgtaat gccggcttca tctccccttt cttccacgcc atcagtaaag caatggaagg
    37621 cacatttccg gcaatgggac gaaaaacaac ctgtccggta ttaaaattat tcatataacc
    37681 gggtatcaaa gtgacgccca gccccatgcc caccagattc atggtcacca gaatattcgt
    37741 tgccacctgg acgatatttg gctggctgtt ttcttgcgca aaccacgctt taacgatcgg
    37801 cgcaagcgaa ccggaatacg ccggatcggt actgacgaaa ttcacgccat ccagttgcgc
    37861 ggcggtgatc tctttttcat gtgctaaagg gtgatcaacc ggtaacacaa ccactaatgg
    37921 ttcgtcaaaa agctccagat aatcaatctc cgggctataa acaggatggc gcattaagcc
    37981 gacgtcgagt tcaccacggc gaattttttc ctcctgttgc gtggtgatta aactcaccag
    38041 ctcaatcaag gtgtctggct gtctgagacg aaacatcggt aatacttttg gcagtaaatt
    38101 cacttccgcc gatggcacaa agccaatggt taattgtctg tcttcctgaa cagttttccg
    38161 cgcccgtaat ttggcatttt ccgcttgttc gagaattgcc agcgcgtcct ggagaaaaca
    38221 ttctcccgcc gccgtcaacg cgactttgcg cttatccctc accagtaacg gaacaccgac
    38281 acagttttca agatcgcgga tctggctgct taacgaaggc tgtgaggtat gcagtttttc
    38341 cgccgcacgg gtaacgttca gtgcctgcgc cactgcgacg aaatagcgta aatgccgtag
    38401 ttccatcacc ttccccttgt tatcgaaaaa acgtctcagc tggtagaaat taactatttc
    38461 acatattagc aaccaaccag caatatcctt atggcacaaa aatagaaggt caatacatct
    38521 tatctttcag gattaaaaaa tatgaccaca ccctcagatt tgaacattta ccaactgatt
    38581 gacacccaaa atggtcgggt cactccgcgt atttataccg acccggatat ttaccaactg
    38641 gagcttgagc gtattttcgg tcgttgctgg ctatttctcg cccacgaaag ccagatccca
    38701 aaacccggtg atttctttaa cacctacatg ggagaagatg cggttgtcgt agtgcgtcag
    38761 aaagacggca gcatcaaggc gtttctcaac caatgccgcc accgggccat gcgtgtgagt
    38821 tatgcagatt gcggcaacac tcgcgccttt acctgcccgt atcacggctg gtcttatggc
    38881 attaacggcg agttgatcga tgtaccgctg gaacctcgcg cctacccaca agggttgtgt
    38941 aaatcccact gggggctaaa cgaagttcct tgtgtggaga gttataaagg gctgattttt
    39001 ggcaactggg ataccagcgc accgggcctg cgtgattacc ttggtgacat tgcctggtat
    39061 ctggatggca tgctggatcg tcgcgaaggc ggcaccgaaa ttgtcggcgg cgtacaaaag
    39121 tgggtgatca actgtaactg gaaatccccg gcagagcagt tcgccagtga ccaatatcat
    39181 gctctgttca gccatgcttc tgccgttcag gtattagggg cgaaagatga tggcagcgat
    39241 aagcgtctcg gtgatggaca aaccgcccgc ccggtgtggg aaaccgccaa agatgcgctg
    39301 caatttggtc aggacggtca cggtagcggt ttcttcttta ctgaaaaacc ggatgctaat
    39361 gtctgggtcg atggcgcggt ttccagctat tacagcgaaa cctatgccga agcagaacaa
    39421 cgtttaggtg aagtttgcgc cctgcgcctg gcgggtcata acaatatatt ccccacgctt
    39481 tcatggctaa acggcactgc cacactccgc gtctggcatc cgcgcggccc tgatcaagtt
    39541 gaagtgtggg cgttctgtat tactgacaaa gccgcctccg atgaagttaa agccgctttt
    39601 gaaaacagcg ccactcgtgc ttttggtcct gctggttttc tcgagcagga tgactcggag
    39661 aactggtgtg aaatccagaa attgcttaaa ggccaccgcg cccgcaacag caaactgtgt
    39721 ctggaaatgg ggcttggtca ggaaaagcgc cgcgacgacg gcattcctgg cattactaac
    39781 tatatctttt cagaaactgc cgctcgtgga atgtaccaac gctgggccga tcttctgagt
    39841 agcgaaagct ggcaggaagt gctcgataaa accgccgctt accagcagga ggtgatgaaa
    39901 tgagtgcgca agtttcacta gagttacatc tccgcattag ccagtttctc tttcacgaag
    39961 ccagcttact ggacgactgg aaatttcgtg actggctggc gcagctcgac gaagagattc
    40021 gttacaccat gcgcaccaca gttaacgcgc aaacacgcga ccgccgcaaa ggcgtccagc
    40081 caccgacaac ctggattttt aatgacacca aagaccagct ggagcggcga atcgcccgtc
    40141 tggaaacggg catggcctgg gcagaagagc cgccgtcacg cacccgtcac ttaatcagca
    40201 actgccagat aagcgaaacc gacatcccaa acgtatttgc tgtgcgggta aattatctgc
    40261 tttatcgggc acaaaaagag cgcgatgaaa cattctatgt tggaacgcgt ttcgacaaag
    40321 ttcgccgtct ggaagatgac aactggcgct tgctggaacg ggatatcgtc ctggatcaag
    40381 cggtaatcac ttcccataac ctgagtgtac tgttctgatg aatcgaattt atgcgtgtcc
    40441 cgttgcggat gtgccggagg gtgaggctct ccggatcgat acctcgcccg tcatcgccct
    40501 gttcaacgtt ggcggcgagt tttatgccat taacgatcgt tgcagccatg gtaatgcgtc
    40561 gatgtcagaa gggtatctgg aagatgacgc cacggtggag tgcccgctac acgccgccag
    40621 tttttgcctg aaaacgggga aagcgttatg cctgcccgcc accgatccgc tcaccactta
    40681 tccagtacac gttgaaggtg gtgacatttt catcgactta ccggaggcgc agccatgagc
    40741 gatctgcata acgagtccat ttttattacc ggcggcggat cgggattagg gctggcgctg
    40801 gtcgagcgat ttatcgaaga aggcgcgcag gttgccacgc tggaactgtc ggcggcaaaa
    40861 gtcgccagtc tgcgtcagcg atttggcgaa catattctgg cggtggaagg taacgtgacc
    40921 tgttatgccg attatcaacg cgcggtcgat cagatcctga ctcgttccgg caagctggat
    40981 tgttttatcg gcaatgcagg catctgggat cacaatgcct cactggttaa tactcccgca
    41041 gagacgctcg aaaccggctt ccacgagctg tttaacgtca acgtactcgg ttacctgctg
    41101 ggcgcaaaag cctgcgctcc ggcgttaatc gccagtgaag gcagcatgat tttcacactg
    41161 tcaaatgccg cctggtatcc tggcggcggt ggcccgctgt acaccgccag taaacatgcc
    41221 gcaaccggac ttattcgcca actggcttat gaactggcac cgaaagtgcg ggtgaatggc
    41281 gtcggcccgt gtggtatggc cagcgacctg cgcggcccac aggcgctcgg gcaaagtgaa
    41341 acctcgataa tgcagtctct gacgccggag aaaattgccg ccattttacc gctgcaattt
    41401 ttcccgcaac cggcggattt tacggggccg tatgtgatgt tggcatcgcg gcgcaataat
    41461 cgcgcattaa gcggtgtgat gatcaacgct gatgcgggtt tagcgattcg cggcattcgc
    41521 cacgtagcgg ctgggctgga tctttaagga agcacgatga aagaaaaaac gatcattatt
    41581 gtcggtggcg tgcaagcggc ggcaatggct gcggcctcgc tacgccagca agggttcacc
    41641 ggtgagctgc atctgttttc cgatgagcga catcttcctt atgaacgacc tccactctcg
    41701 aaatccatgt tgctggaaga ttccccgcag ttacagcagg tgttacccgc taactggtgg
    41761 caggaaaaca atgttcatct gcattccggt gtaaccatca aaacgctggg tcgcgacaca
    41821 cgagagttag tgttaaccaa cggcgaaagc tggcactggg atcagctttt tatagcaacc
    41881 ggcgcggcag ctcgaccgct gccgttgctt gatgcactgg gagaacgctg ctttacccta
    41941 cgccatgccg gtgatgccgc cagactgcga gaagttctgc agcccgaacg gtcagtcgtg
    42001 attgtcggtg ccggaactat tggtctggaa ctggctgcca gcgccacgca gcgcagatgt
    42061 aaggtgacag tgattgaact ggcggcaacc gtcatgggcc gtaatgcacc accgcccgtg
    42121 caacgctatc ttttacagcg ccaccagcag gctggtgtgc gcattctgct caataatgcc
    42181 attgaacatg tggtcgatgg tgaaaaagta gaactgacgc tgcaaagtgg ggaaacgctt
    42241 caggctgatg tggtgattta cggtattggt atcagcgcca acgagcaact ggctcgcgag
    42301 gccaaccttg atactgccaa tggcattgtc attgatgagg cttgccgcac ctgcgatccc
    42361 gcgatctttg ccggtggcga tgtggcaatc actcgtcttg ataatggtgc actacaccgc
    42421 tgcgaaagct gggaaaacgc caataaccag gcgcaaattg ccgctgccgc aatgttaggg
    42481 ctaccgctac tgccgccgcc gtggttctgg agcgatcagt acagtgataa cttacagttt
    42541 attggcgata tgcgtggcga t
//
LOCUS       DAJTNA010000011        41823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTNA010000011 DAJTNA010000000
VERSION     DAJTNA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41823
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.011"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..527
                     /locus_tag="OO161_000511"
     CDS             <1..527
                     /locus_tag="OO161_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5461692.1"
                     /translation="KYKIMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAEI
                     INKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHN
                     GNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRN
                     GLMPLIRRRTMLQL"
     gene            893..1960
                     /locus_tag="OO161_000512"
     CDS             893..1960
                     /locus_tag="OO161_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5461693.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASEAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1957..4692
                     /gene="rbbA"
                     /locus_tag="OO161_000513"
     CDS             1957..4692
                     /gene="rbbA"
                     /locus_tag="OO161_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5461694.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGIGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4692..5816
                     /locus_tag="OO161_000514"
     CDS             4692..5816
                     /locus_tag="OO161_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5461695.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5889..6164
                     /locus_tag="OO161_000515"
     CDS             5889..6164
                     /locus_tag="OO161_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5461696.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6161..6520
                     /locus_tag="OO161_000516"
     CDS             6161..6520
                     /locus_tag="OO161_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5461697.1"
                     /translation="MIKLKTPNTMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="OO161_000517"
     CDS             complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="OO161_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5461698.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="OO161_000518"
     CDS             complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="OO161_000518"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5461699.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGYISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="OO161_000519"
     CDS             complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="OO161_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5461700.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADELTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="OO161_000520"
     CDS             complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="OO161_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5461701.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="OO161_000521"
     CDS             complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="OO161_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5461702.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="OO161_000522"
     CDS             complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="OO161_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5461703.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="OO161_000523"
     CDS             complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="OO161_000523"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5461704.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12715..13764)
                     /locus_tag="OO161_000524"
     CDS             complement(12715..13764)
                     /locus_tag="OO161_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5461705.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13863..15107
                     /locus_tag="OO161_000525"
     CDS             13863..15107
                     /locus_tag="OO161_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5461706.1"
                     /translation="MKHCCKNVVILIPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGT
                     RLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPAL
                     GVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIA
                     LLLTWRLKKRPPEHVPEAASSS"
     gene            complement(15111..15668)
                     /gene="dcrB"
                     /locus_tag="OO161_000526"
     CDS             complement(15111..15668)
                     /gene="dcrB"
                     /locus_tag="OO161_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5461707.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSHDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15741..16406)
                     /locus_tag="OO161_000527"
     CDS             complement(15741..16406)
                     /locus_tag="OO161_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5461708.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILNVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16627..16872
                     /gene="tusA"
                     /locus_tag="OO161_000528"
     CDS             16627..16872
                     /gene="tusA"
                     /locus_tag="OO161_000528"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5461709.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16974..19172)
                     /gene="zntA"
                     /locus_tag="OO161_000529"
     CDS             complement(16974..19172)
                     /gene="zntA"
                     /locus_tag="OO161_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5461710.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCATCARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDGQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19246..19872)
                     /locus_tag="OO161_000530"
     CDS             complement(19246..19872)
                     /locus_tag="OO161_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5461711.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20013..20372
                     /locus_tag="OO161_000531"
     CDS             20013..20372
                     /locus_tag="OO161_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5461712.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRCVSFVGEQ"
     gene            complement(20375..20644)
                     /locus_tag="OO161_000532"
     CDS             complement(20375..20644)
                     /locus_tag="OO161_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5461713.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20634..21230)
                     /gene="rsmD"
                     /locus_tag="OO161_000533"
     CDS             complement(20634..21230)
                     /gene="rsmD"
                     /locus_tag="OO161_000533"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5461714.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21380..22882
                     /gene="ftsY"
                     /locus_tag="OO161_000534"
     CDS             21380..22882
                     /gene="ftsY"
                     /locus_tag="OO161_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5461715.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFI
                     SLFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKE
                     EMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDT
                     FRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQN
                     KSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTK
                     LDGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22885..23553
                     /gene="ftsE"
                     /locus_tag="OO161_000535"
     CDS             22885..23553
                     /gene="ftsE"
                     /locus_tag="OO161_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5461716.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23546..24604
                     /gene="ftsX"
                     /locus_tag="OO161_000536"
     CDS             23546..24604
                     /gene="ftsX"
                     /locus_tag="OO161_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5461717.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRVKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24849..25703
                     /gene="rpoH"
                     /locus_tag="OO161_000537"
     CDS             24849..25703
                     /gene="rpoH"
                     /locus_tag="OO161_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5461718.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25974..27077
                     /gene="livJ"
                     /locus_tag="OO161_000538"
     CDS             25974..27077
                     /gene="livJ"
                     /locus_tag="OO161_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5461719.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27276..27659)
                     /gene="panM"
                     /locus_tag="OO161_000539"
     CDS             complement(27276..27659)
                     /gene="panM"
                     /locus_tag="OO161_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5461720.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVILSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKH"
     gene            28083..29192
                     /gene="livK"
                     /locus_tag="OO161_000540"
     CDS             28083..29192
                     /gene="livK"
                     /locus_tag="OO161_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5461721.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            29240..30166
                     /gene="livH"
                     /locus_tag="OO161_000541"
     CDS             29240..30166
                     /gene="livH"
                     /locus_tag="OO161_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5461722.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            30163..31440
                     /gene="livM"
                     /locus_tag="OO161_000542"
     CDS             30163..31440
                     /gene="livM"
                     /locus_tag="OO161_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5461723.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            31437..32204
                     /gene="livG"
                     /locus_tag="OO161_000543"
     CDS             31437..32204
                     /gene="livG"
                     /locus_tag="OO161_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5461724.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            32206..32919
                     /gene="livF"
                     /locus_tag="OO161_000544"
     CDS             32206..32919
                     /gene="livF"
                     /locus_tag="OO161_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5461725.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRAASGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            33316..34633
                     /gene="ugpB"
                     /locus_tag="OO161_000545"
                     /pseudo
     CDS             33316..34633
                     /gene="ugpB"
                     /locus_tag="OO161_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            34731..35618
                     /gene="ugpA"
                     /locus_tag="OO161_000546"
     CDS             34731..35618
                     /gene="ugpA"
                     /locus_tag="OO161_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5461726.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            35615..36460
                     /gene="ugpE"
                     /locus_tag="OO161_000547"
     CDS             35615..36460
                     /gene="ugpE"
                     /locus_tag="OO161_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5461727.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAAWIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            36462..37520
                     /gene="ugpC"
                     /locus_tag="OO161_000548"
     CDS             36462..37520
                     /gene="ugpC"
                     /locus_tag="OO161_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR5461728.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEVELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSN
                     LDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEV
                     YEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKMTLGI
                     RPEHIVLSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGSTLWL
                     HLAENQLHLFDGETGQRV"
     gene            37517..38260
                     /gene="ugpQ"
                     /locus_tag="OO161_000549"
     CDS             37517..38260
                     /gene="ugpQ"
                     /locus_tag="OO161_000549"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5461729.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(38247..38687)
                     /locus_tag="OO161_000550"
     CDS             complement(38247..38687)
                     /locus_tag="OO161_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5461730.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSKPQPDIPLKTIGP"
     gene            38807..40540
                     /gene="ggt"
                     /locus_tag="OO161_000551"
     CDS             38807..40540
                     /gene="ggt"
                     /locus_tag="OO161_000551"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5461731.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAVAAPPVSYGVEEDIFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIHSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(40578..40862)
                     /locus_tag="OO161_000552"
     CDS             complement(40578..40862)
                     /locus_tag="OO161_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HCR5461732.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(41062..41217)
                     /locus_tag="OO161_000553"
     CDS             complement(41062..41217)
                     /locus_tag="OO161_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461733.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
     gene            complement(41312..41806)
                     /locus_tag="OO161_000554"
     CDS             complement(41312..41806)
                     /locus_tag="OO161_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461734.1"
                     /translation="MMTKTQINKLIKMMNDLDYPFEAPLKESFIESIIQIEFNSNSTN
                     CLEKLCNEVSILFKNQPDYLTFLRAMDGFEVNGLRLFSLSIPEPSVKNLFAVNEFYRN
                     NDDFINPDLQERLVIGDDSISIFTYDIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIM
                     DSCS"
BASE COUNT         9806 a        10669 c        11616 g         9732 t
ORIGIN      
        1 ccaaatacaa aataatggca aatcatcatg ggataatttt tgatgagcag cgaaaacgtt
       61 acattatagg atcgataaca gtctctattg atgaaatgac caatgctctc gatccggtcg
      121 aattagcgga aattattaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa
      181 atagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca
      241 gacctaaact tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg
      301 cacataacgg taatggtgga agagaatgta taggtgaaac ggggacagtt ttaggtctta
      361 acgcccgtga cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc
      421 ctggtgcagg gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa
      481 atggacttat gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca
      541 tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg
      601 ttcctgcaac tgaacaggcg tgaaacgcga tccaacagga tggataccgt tttgtcagcc
      661 agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga
      721 tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg
      781 ctggtcgtat ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg
      841 gcggcagttg taacacgcgt cgtttgctta cagggattat ggcgggccga ttatggataa
      901 gagtaagcgc catctggcgt ggtgggttgt cggggcactg gcggtggctg ccgtcgtggc
      961 gtggtggctg ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat
     1021 tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt
     1081 gaaagaaggc cagtttgttc gcgaaggtga agtgctggcg aagatggata ctcgcgtgtt
     1141 gcaggaacag cgactggaag ccatcgcgca aatcaaagag gcacaaagcg ccgttgccgc
     1201 cgcgcaggct ttgctggagc aacgacaaag cgaaactcgc gccgcgcagt cgctggttaa
     1261 tcaacgccag gcagaactgg actccgtagc aaaacgtcat atgcgttccc gttcactggc
     1321 ccaacgaggg gctatttctg cgcaacagct ggatgacgac cgcgccgccg ctgagagcgc
     1381 ccgagctgcg ctggaatcgg caaaagctca ggtttctgct tctgaagccg ctatagaggc
     1441 ggcacgtacc aatatcattc aggcgcaaac ccgcgtcgaa gcggcacaag ccactgaacg
     1501 gcgcattgcc gcagatatcg atgatagcga actgaaagcc ccgcgtgacg gacgcgtgca
     1561 gtatcgggtt gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt
     1621 cgatctcagc gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctaaa
     1681 actgggcggt gaagcacggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac
     1741 catcagtttt gtcgccagcg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga
     1801 acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca
     1861 tctggaatat gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga atgaagaact
     1921 tccgtggcct gacgacctgg tggtgaggtt gccgcaatga cgcatctgga actggttccc
     1981 gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg
     2041 aacaatatca ctctagatat tccggcccgc tgtatggtcg ggctgattgg cccggacggc
     2101 gtcgggaagt cgagtttgtt gtcgttgatt tccggtgctc gtgtcattga gcagggcaat
     2161 gtgatggtgc tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc
     2221 atcgcctgga tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa
     2281 aacgtcgatt ttttcgcccg cctgttcggt cacgacaaag cggagcggga agtgcgaatt
     2341 aatgagctgc tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc
     2401 tccggcggga tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg
     2461 ttgatccttg atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat
     2521 ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat
     2581 atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca
     2641 accggcagcg ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt
     2701 attaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat
     2761 caacctgaaa acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc
     2821 ttcgttgccg ttgatcacgt caatttccgc attccacgcg gggagatttt tggattcctt
     2881 ggttcgaacg gctgcggtaa atccaccacc atgaaaatgc ttaccggact gctgcccgcc
     2941 agcgaaggtg aggcgtggct atttgggcaa ccggttgatc caaaagatat cgatacccgc
     3001 cgtcgtgtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa
     3061 aacctggagt tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg
     3121 gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg
     3181 ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg
     3241 ttaatcctcg atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag
     3301 ttgatggtcg atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg
     3361 aacgaagcgg aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc
     3421 ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc
     3481 gcctatttgc aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac
     3541 gacaccaccc acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc
     3601 cgcgaagcgc tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg
     3661 gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt
     3721 gcggtgctcg accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt
     3781 tcccgttact ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg
     3841 cgtgcgggcg atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg
     3901 cgtggtacgc ccgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa
     3961 acggtaaaag gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga
     4021 caatcgacac ccgccagcca aagcgggctg atgaacattg agacgcgtta tcgctataac
     4081 ccggacgtaa aaagcctgcc agcaattgtt ccggcggtga tcccgcttct gctgatgatg
     4141 atcccgtcaa tgctaagcac ccttagcgtg gtgcgggaaa aagagcttgg gtcgattatc
     4201 aacctttacg tgacccccac cacgcgtagt gaatttttgc ttggtaaaca gttgccatac
     4261 atcgcgctgg ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc
     4321 gtaccgcata aaggcagttt cctgacgctc accttggcgg cgctgctgta tatcatcatt
     4381 gccaccggaa tagggctgct gatctccacc tttatgaaaa gccagattgc cgcaattttc
     4441 ggaacggcga ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta
     4501 gcttcgctgg aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg
     4561 actatcgccc gcgggacgtt ctcgaaagcg ctggatctga ctgatttgtg gcaacttttt
     4621 atcccgttgc tgatagccat cccgctggtg atgggcttaa gtatcctgct gctgaaaaaa
     4681 caggagggat gatgcgccat ttacgcaata tttttaatct gggaatcaaa gagttgcgca
     4741 gtctgctcgg tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt
     4801 attcgtcagc gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata
     4861 tggatcaatc gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc
     4921 caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct
     4981 tcgcgataaa tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata
     5041 ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc
     5101 agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac
     5161 cgttggtatc gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg
     5221 gcggggtgat ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg
     5281 cgctgatccg cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc
     5341 cgtttgagat catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg
     5401 gattatcgct ggtgctgatg gtgaaaggtg tactgggcgt accgattgaa ggctcgatcc
     5461 cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta
     5521 tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc
     5581 cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg
     5641 acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc
     5701 ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg
     5761 cgtttttcac cattgcgctg ctgcgattta ggaagacgat tgggacaatg gcgtaaggaa
     5821 gggaaacttg acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag
     5881 gacatgccat gagaaccaag gtacaggctt tacggaagaa acaaaaaaat acattggatc
     5941 aaatatttaa aactcctgtt cctcaaggga tcaagtggtc tgatatagag tcactggtta
     6001 aagcattagg cggagaaatt aaggaaggaa gaggttcgcg ttgtaagttc atactaaata
     6061 tgagcgttgc gtgtttccat cggcctcatc cgtcgccaga taccgataaa ggcgctgtag
     6121 aaagcgtgcg tgactggtta ctaagtatag gggtaaaacc atgatcaaac tgaagacgcc
     6181 aaacactatg gagattgccg ggcaaccagc tgttattacc tacgtgccag aactgaatgc
     6241 atttcgtggc aaattcctgg gcttgtcagg ttattgtgat tttgtctctg acagcattca
     6301 ggggctgcaa aaagaagggg aactttccct gctagagtat cttgaggatt gtaaagctgc
     6361 gggtattgag ccgtatgcgc gtacagaaaa gattaagacc tttacgttac gctatccaga
     6421 atcgctgagt gaacgcctta ataatgcggc tgcgcagcaa caagtctctg ttaataccta
     6481 tattattgaa acactgaatg agcgtttgaa tcatttataa attttagcgg cgtacattac
     6541 gcagaatatc tgcctttaca ggatctatct atgcaactga acttaatttt ataaattaag
     6601 ttcagttaat atcttttctt tcctcgtttc agaaataact caatcttcct tcggcaagca
     6661 ctgcaaatgc ccatgccgca cgccgcgctg ggcgataacg tcatcggcaa aatgctgcac
     6721 gtcgcccata tcacctttca gcacggcgat ttccagacag tcgtcgtgat tgatatgcac
     6781 atgcagcgtg gcgacggaga gatcgtggtg atggtgctgg gtggagacaa tgcggctggc
     6841 taagtcgcgt ttttcgtgtt catacacata ggagagcacc gcgaaacctt gcgtgccgtg
     6901 ctgctgagtg gcctcctggg ccagggcgct acgcagaata tcgcggatag cttcggaacg
     6961 gttgttataa ccacgacgct ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag
     7021 cgtgatggtg actcgttgca tttgcgttaa accttttctg tggcgcgacg gcgcacgggg
     7081 aatgcgggta ataccgcgtt ttgtagcaca cgtccggcgt cagaggaaaa ggttaattta
     7141 tctcccaccg cctgggtttc gacgatttgt ccgttgtcca tcaccatcac ccgctggcaa
     7201 aagcgttcta caagacgtaa gtcgtgggtg atgaacaggc aggcggtgcc aaactgttgt
     7261 tgcagctttt tcagcaggcg aatgacgccc gcctgtaaca cgagatcaag gttagaaacg
     7321 gtttcatcca gaatcagcaa tttcggttcg accgccagcg cgcgtgccag gcagacgcgc
     7381 tggagctggc cgccgcttaa ctgcggtggg cgtttgtcga gaacgctgtc atcgagatcg
     7441 accgccttca gcatttcgct ggcgcgcgcc agttgttcgg attttttcag tgatagcagg
     7501 tggcgcatcg gttcacgcag gatctcgcgc acggttttgc gcggattcac ggcactgatg
     7561 gagtcctgaa acaccatctg aatatcacgg cggaaggctt tacgttgggc gcgattaagt
     7621 ttcgccagcg attcgccgcg ccagctaata tacccttgtg agggagactc taagcctacc
     7681 agcagccgcg cgagggtgct ttttccgcag ccgctgcgtc ccagcagggc gacggtttcg
     7741 ccgcttttca gggtcaggga aacgttattc agcaccgcct gatgttgatg ttttccgcta
     7801 aatccaccgt gggcatagtg atgggaaagg ccgctgacgt taagtaaggt catgatgcca
     7861 gctccatacc gtagagggcg agatgagcgg aaaccaggct gcgcgtcacc gtatgtttgg
     7921 gggcgttaaa cagcgtttct acatcgccct gttcgacaat tttaccttgt gacatcaccg
     7981 ccacgtcatc cgccagacgc gccacaacgc ccatatcatg ggtgaccagc agcattcccg
     8041 gcgcttgttt ttgcataatg ctttccagca gatcgaggat gcgcgcctgt gctaccacgt
     8101 cgaggtcggt ggtcagttca tcggcgatga taaacggtga ttcacacagc accgccatcg
     8161 caatcatcat gcgctgcaac atgccgccgc tcatctcgaa cgggtacagc ttcagcacgc
     8221 gcgcggcgtt ttccagcccc accgcttcta tggcagcggt aagcgtagcg tcatcggcgg
     8281 gtttccctaa cgccaggcag gtttcccgcg cgtgggtgtg catggtgtgt agcggattaa
     8341 aggcgctgcg cgggttctgc atgatggtgg caattttgat gccgcgcagg gcgcagggat
     8401 aaaccgcttt gccatcggct aaaatttccc ccgccgtctg gcgaacgcca gcgggcaaaa
     8461 tgcccagcgt cgcggcgcag gttaatgatt tcccgctgcc gctaccgccg actaacgcca
     8521 gcacgcgccc gcgttgcagg gttaacgata caccgtgtac cagcggctgc gcggcctgta
     8581 gcgcgatatt acgtagttca atctgttgcg gcattagtgt gcgtgctccg tcaccagatg
     8641 agggtccaga tgatcgcgca gtgcgtcacc caccaggtta aaggccatca cgctgataaa
     8701 cagcgccagc cccggccaga acatttgcag cggctgggtc cagatatact ggcgcgcgtc
     8761 gttaatcatc acgccccatt cggcggtcgg cgcggtcaca ccgaggccaa ggaaagacat
     8821 ccccgagacg tgcagcatca tatggccgat atccagcgtt gccagcacca gcagcgaagg
     8881 gatcaccgcg cctgccagat gatcgacaaa cactcgcaca tggcccgcac cggaaagccg
     8941 tgacgccagc acaaactcgc gttggcgtag tgaaatcacc aggctgcgca ccatgcgtgc
     9001 ataccacgcc cagtgcgaca gggcgatggc gataattacg ttggtcagcc cggtgccgag
     9061 cacgccaacc ataaagaacg acagaatcga ggtcgggaag gtcataaaca tatcggcgac
     9121 gcgcatggtg gcctgatcaa cgcgcccgcc aatcaacccg gcgctgccgc caataaccag
     9181 ccctaatgtc agcaccagca gcaggcaggc cattaccgaa ccgagcgaca cgcgggtcgc
     9241 tgccatcagc cgcgagaaaa tatcgcgacc taagtgatcg gtgcccagcc agtgctgcgc
     9301 atccggcgaa agcaggcgcg acggcaaatc aatcgcctgg gggtcatacg gcagccacca
     9361 ctggctggtg agcgcaatca gcgccagcag ggcgataatg atcagcgcca ggcgtaccga
     9421 ccagcgggaa gagaggaaaa agttcacgcg tgcgctcctt catgacgacg aatgcgcggg
     9481 tccagcgcgg cgttgagcaa atcgacaatc aaattacaga ccacaaaaac caccaccatc
     9541 atcagcgtaa agcactgaat caccggatag tcacggttaa aaatcgccga caccgcatag
     9601 cgcccgacgc caggccaggc aaagatgttt tcgataatca tcgtcccgcc aatcaattcg
     9661 ccaatgtgca tccccacggc ggtgatcatc ggcagcgagg cattgcgcag aatatggcgg
     9721 cgttcggtct gtttgtcatt caggccgcgt agccgcgccc aggtgacgtg acgctgaccg
     9781 gcgacgtcca gcatactggc gcgcagcagt cgcgcgttaa tcgccagcga cataaaggcg
     9841 atggaaaccg caggcaaaat gatgtgctgc cagccgccgt agcccatcgc gggtagccat
     9901 tgcagataca ccgaaaacgc catcactagc aaaaatgcca gccagaagtt gggcatcgac
     9961 acgccaagaa acgcgataaa gcgcacggcg aaatccggca aacgatcgcg gtggcgcgcc
    10021 gcccagatac cgagcggtac ggaagtgagc agaattaata ccagcgccgc acctgcaagt
    10081 tccagcgtgg cgggcaggaa gttcagcata tcgtccagca ccgggcgttg gctggcgaat
    10141 gagataccaa agtcaagatg cagcgccttc cacaaccagg tgccgtactg gacgtacagc
    10201 ggctgatcca gtccgagcat ggtgcgggta gaggccagca tctccggcgt cggcggcagg
    10261 ttagacagac gcaaataatc gagcgccggg tcgccggtac cgaggcgcag catcagaaaa
    10321 atgatcaccg aggcggcgag caccatcggg atcagcagca gaaagcggcg taatacgtaa
    10381 cgcaacatta aggtttcacc ggtttaatct gttcgaacgg aatttcggtg gcgatcggcg
    10441 cgtaggggat gttacccagc tccggttttg ataccaccat cattgagatg taactgatag
    10501 gcagataaac cgcctcgtca tgcagacggg tcagaatgtc gcgatacagc gcctgacgtt
    10561 gcgtttcgtc atgggtcgcc agcacttcgc cgatctcttt atcaatcagc ggtttgtcgg
    10621 ctaatccttg ctgtgcctgg aagtcagcgt gcgacggtac gcgcattgaa ctcaggaagg
    10681 cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat catgccaaaa cgaccgtcgc
    10741 gctgacgagc atagatactg ctctcttctt cgccaatcag cgagacatct gcgccaatct
    10801 ggcgcatatc agcctgaatg atttccgcca tcgatttgct taacgcatcg gtgccgatga
    10861 acgacagttc aatgcgcagc ggctgaccat ttttctcgcg gatgtctttg cccgcaggca
    10921 gcgtccaacc ggctttttcc agcaacgctt tcgctttctg cggatcgtac tggcgcggtt
    10981 tcaggccgag gttggcgtag ggcacagaag gggcaaacag ggtgtcggcg acctgctggg
    11041 tgccatacaa cgcgttatca atcagcgatt ttttgtttac cgcgtaatta agagcttcac
    11101 gtactgccag ctcattggtg ggggttttgg cggtattaag cgccagcatc acggtttcga
    11161 tcggctgtga cagttgggta tggtaagccg ggttctggct aaagcgggcg aaggtatcga
    11221 gcggtaataa cccttcgttt ccgtacagca ggtcgatatc gccagtttca aacgccaccg
    11281 cgcgggtagt cgggtccggg atgacgttaa aggtgatctt tttaatcgct ggcttttcgc
    11341 cccagtagtt ttcgttacgg acgaagacat cgtactgatt cagtttcgat tcctgcaaaa
    11401 tccacggtcc ggtgccaatc ggcgctttaa ttccgttcat ggtttcatgg tttttaaact
    11461 gcgagggagc gataaaacgg aaaggacggg gcagggccag ttcttgcagg aaaggatagt
    11521 aggcgctttt cagggtaatt tgcagctctg ttttactgag tgctttaaca tcaacaatct
    11581 ggtttgccag ctccagccag gcgtgacgtt gacggttatc gagcactgcg cggaagtttt
    11641 ctgccgccgc ctcggcatcg aacggttcac cgttggataa tttcacgtca tcacgcaggg
    11701 tgaaggtcca ggttttacca tcttctgaat gagtccagct ttttgccagc cacgggatca
    11761 ccgaaccgtc tgcctgatat ttcaccaatg gttcataaac catgctctgg gcgaacatct
    11821 ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt caccggccag gcggtagtga
    11881 tttcatctgg tgcagcggca tggacgataa aagacgcaca agccagcagc gcaaatagag
    11941 tgcggcggag tgtggagagc atgataatcc caatggatta aaatagatgg cggaaataag
    12001 tatgacgatt ttaagtattc gtcatactga ttacctgttc tggatcaata gatgggcagt
    12061 cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca ctgtcggcgg tgagcgtaaa
    12121 gggggtgggg gtgcagatcg caaggcttaa attttctagc tgacaatggc tgactgacag
    12181 cgaggagtga taggtgctat ctacgctgac gatttgccag gcgctgccgc cacgctgttt
    12241 aacgatggct tctttgcgcg tccagatgcg ccagaacatt tccagttgct gatcaggatg
    12301 cacggcgtcc atttcagcgt gttccccgag gctgaacacg gcgttcgcca gccagcgcca
    12361 gttggcgcgc gggcgaatca cttcgatatc gcagccgact tcgccttcat cgctcaacag
    12421 cagagcgata tcgtcaccgc tatggcttaa gttgaaccat agcggcgttt ccggcgcgaa
    12481 tgcaggtttg ccttgttcgc catagatgat ctccggtagc ggggaaagcg tgtgcgaaag
    12541 caatgcacgc cccgccagcc agcgttcgcg tcgtggacct tgcggtgctt gctcgcgtaa
    12601 acccggtggc agtggagctg cgcttaaggt cgaaactttc cccagaacta tccgatacat
    12661 atcagggcca acgtttaatg gaaaatgaaa gtgcgtatcg tatcacttgt cgcctcatcc
    12721 cggtaaccga cttttcggtc tgcctggccc cagtaaaatc gccagtttgc taccgccttt
    12781 ggtggtttcc atccagattt tacacacgct ggttaatggc accgaaagta gcatccctac
    12841 cgggccgagc agccatcccc aaattaacaa tgaaagaaat accaccatgg tggacatccc
    12901 cagtcgatgg cccatcatcc gtggttctaa aatattgccg atgaccatat ggaccactaa
    12961 aaacaatgcg ccgaccagaa tacattcgta aacaccatta aacagcagca cctgaatcat
    13021 tggcggtacg gcggaaatta ccgcgccgat attgggcacg tagttgagca aaaacgccag
    13081 tactgcccac atcagcgcaa actgtacgcc cattagcgcc agccccagcc agacgatgac
    13141 acctgtccat aaactgagta gcgtctttaa cgccagataa tgcgaaacgc ctttaagtgc
    13201 gcggtgtaat cctgcgatgt gaatctgtgg gttattcagc gcaaaacgca ttttgtaagg
    13261 gacgtggcgc acttcaaaca gcataaaaac tacggtcatc accagcaaaa gcacgctcgc
    13321 cattgccccg gaaagcccgg tcattagcgc tgtggtgaag gtaaccactt tttccgagtc
    13381 catccgctgc agcattcgct ccggcgacat atgcaaatta agaaaaggca acatctcctg
    13441 caatttaaaa agtttgcgcg tcagctcctt attaaacttc ggcagcatag agataaattc
    13501 gttaaacgat gccgccagta cgccgaccag cgcggttagt gcgatcagca tcaccaccac
    13561 tacaatcgta atggcaacgg ggcgttgtac tccccgacga ataaaccagg taacgagcgg
    13621 gttgaggacg atggcaaaaa acagcgccag taacagctgc acaatgatat ctgccgctgc
    13681 gtgaatgccc gcgaggatca ctaccagcga ggccaacttg agcagaatgt gcatgcccgt
    13741 tttatcgggt tgaggggttt ccattggggc ttccttgtga ctttttgtgt taagtgtagc
    13801 gggagtcacg ccagcaatat tctgattctt actgctgatt ttcacgtcag cacgtggtaa
    13861 aaatgaaaca ctgttgtaaa aatgtggtga tcctcatacc cgaacccgta gccgaacccg
    13921 cgctaaacgg attgcgcctg aatttgcgca ttgtctccat tgtcatgttt aacttcgcca
    13981 gctacctcac catcgggttg ccgctcgctg tattaccggg ctatgtccat gatgtgatgg
    14041 gctttagcgc tttctgggca ggattggtta tcagcctgca atatttcgcc accttgctga
    14101 gccgtcctca tgccggacgt tacgccgatt tgctgggccc caaaaagatt gtcgtcttcg
    14161 gtttatgcgg ctgctttttg agcggtctgg gatatctgac ggcaggatta accgccagtc
    14221 tgcccgtcat cagcctgtta ttactttgcc tgggacgcgt gatccttggg attgggcaaa
    14281 gttttgccgg aacgggatcg accctgtggg gcgttggcgt ggttggctcg ctgcatatcg
    14341 ggcgagtgat ttcgtggaac ggcattgtca cttacggggc gatggcgatg ggtgcgccgt
    14401 taggcgtcgt gttttatcac tggggcggct tgcaggcgtt agcgttaatc attatgggcg
    14461 tggcgctggt ggccattttg ttggcgatcc cgcgtccgac ggtaaaagcc agtaaaggca
    14521 aaccgctgcc gtttcgcgcg gtgcttgggc gcgtctggct gtacggtatg gcactggctt
    14581 ctgccggatt tggcgtcatc gccaccttta tcacgctgtt ttatgacgct aaaagttggg
    14641 acggtgcggc tttcgcgctg acgctgttta gctgtgcgtt tgtcggtacg cgtttgttat
    14701 tccctaacgg cattaaccgt atcggcggct taaacgtagc gatgatttgc tttagcgttg
    14761 agataatcgg cctgctactg gttggcgtgg cgactatgcc gtggatggcg aaaatcggcg
    14821 tcttactggc gggggcaggg ttttcgctgg tgttcccggc attaggtgta gtggcggtaa
    14881 aagcggttcc gcagcaaaat cagggggcgg cgctggcaac ttacaccgta tttatggatt
    14941 tatcgcttgg cgtgaccgga ccactggctg ggctggtgat gagctgggcg ggcgtaccgg
    15001 tgatttatct ggcggcggcg ggactggtcg caatcgcgtt attactgacg tggcgattaa
    15061 aaaaacggcc tccggaacac gtccctgagg ccgcctcatc atcttaaaac ttactgaata
    15121 accagcgtat taatgatgtt ttctgcggtg gtctgcgctt tttgctgatc gtcagcgggc
    15181 agcgtaattt gcatggtcag cagttgatta cccacgttac ccagaataac ggaagagtac
    15241 gccgtctggc ctttcgcgga gataatactg tctaactgct gcattttgtg acctttcagc
    15301 tcaatggctt tattggttac cacttgcagc tgcggatcgt ggctacgttg ctgatcttcc
    15361 agacgcttcg ccagcaccgc cagatcttct ttcggatcat cgcccatgat gacgatgact
    15421 gctttctgcc cggtggcgtc ggaccagaca tgcatgttat tggcctgcgt tcccagctta
    15481 ccgctctggt cggtcatatc cgctggcagc gagaaactta acttgccatc aagcaggttg
    15541 acgggattcc cggtagcgtt actttccgcg accgaaccct gcgccgtagc gttagtgtct
    15601 ttatcatcac aggccgcaag ccccataacc agcaggccaa ttccgacata tttaaccaga
    15661 ttgcgcattg acttcttcct ttcgataaac ggccataacg gctcattcat ccatcttatc
    15721 acaactctga taacgaacct ttaactcgcc tgcaaagcgt tgatttcgga tttatctgcc
    15781 agtcttttca acagcatatt gagtaatacg ccatacattg gcaggaagaa aacgatactg
    15841 attaacactt tgaaacagta atcgaccagc gcgatttcca tccagtgttc agccataaag
    15901 gcatccgggc tacgccagaa ggcaatgaag aaaaaggcca gcgtgtcgct gacgttaccg
    15961 aacagtgtgg acgctgtcgg tgccagccac cagcggcgac tctgacgcag gcggttaaaa
    16021 acgtgcacgt tgaggatctg tcccagcgcg taggccatga aactggcggt ggcgatacgg
    16081 gcgacaaaca ggttgaagtg ggcgagtgcg ccgaatccct gccaggaacc catatagaat
    16141 agcgacgaga tgacgtagga gatcagcaac gccgggatca tcaccgcaaa gatgatacga
    16201 cgggccagcg gtgcgccaaa aatacgcacg gtcaggtcgg tagcaaggaa aataaacggg
    16261 aagctaaacg cgccccaggt ggtatggaaa cccaaaatgg agacgggaag ctgaaccaga
    16321 tagttactgg aggtgatcac cagcagatga aataacgata accagaacaa cgccttatag
    16381 cgttgagttt gcgagaaaac gttcatattg tacctttttg attaaccatt ggggtgaggg
    16441 aacccaatac gtacgacacg tctgttacct taacataata acgtgcttac ccttttttcg
    16501 agccgcagca tgatactgct ttacgttgac aatgcaatgg ttaattttca cgcaatcgtt
    16561 aacctggttt gcttacggac tcacagggcg taaactagcg ccgttttttt atgtgatgag
    16621 aagaaaatga ccgatctctt ttccagccct gaccacacac tcgacgcact tggcctgcgc
    16681 tgcccggaac ccgtaatgat ggtgcgcaaa actgtgcgca atatgcagcc cggcgaaacg
    16741 ttgctgatta tcgccgacga tccggccact acccgcgata ttcctgggtt ttgtaccttt
    16801 atggaacacg aactggttgc gaaagagacg gatggactgc cttatcgtta tttgattcgt
    16861 aaaggcggtt gatgggggct gattggcttc gatgccgcct tttcccctca ccctaaccct
    16921 ctccccagag gggcgagggg accgatcgcg ctcaatgttg cgatcggttt gccttatctc
    16981 ctgcgcaaca atcttaacgc attcgctgtc accagcaccg tcgcacccgt atctgccagc
    17041 accgccagcc atagtccggt catccctaaa agcgtggtga cgaggaagat ccctttcagt
    17101 cccagcgcaa tggtgatgtt ctggcggata ttggcgtgag tggcgcgtgc cagttcaatc
    17161 atttgcacca ggccgcgcag gtggttatgg gttaatgctg catcagcggt ttccagcgcc
    17221 acgtctgtgc cactgcccat tgcaatccca atggcggcag ctttcatcgc tggcgcgtcg
    17281 ttaataccgt caccgaccat cgccagcggc gcatgttgat tcagctcggt caccgctttg
    17341 actttatctt ccggcaacag gcccgcttta aactccagcc ccagctcccc ggcaattgcc
    17401 gccgctgcgc gtggattatc gccggtgagg atcacccctt tgacgcccag cgcgttcagt
    17461 tcactgatgg cagttgcagc atcggcgcgc agggtatcct gcaatgcaat gatacccagc
    17521 acgtcatcat tacgtactac cagcacaacc gtttgcccgg cgctttccag ttcattaatc
    17581 aaaccagcaa atgcatcagc gggatgtttc ccggcagcgc atatcaacac gcgctcaccg
    17641 ttaacctgtg cttcaatgcc agacccgacc agcgcccgct gtgattcggc ggcgggaatg
    17701 gcgagtgcag caacctgtgc ttcgcgcacg atggcctgcg ccagtggatg cgtcgcgctc
    17761 tgctcgaccg ccgccgccag tgtcagcagt tcagattcac taatacccgt tgccggatga
    17821 atcgcggtaa cgcgcggttt accgacggtc agcgtaccgg ttttatcaaa cgccacctga
    17881 gtaacacgac ctagttgttc cagcgccgct ccgcctttaa tcaacgcccc acgacgcgct
    17941 gctgccgcca gcccggaggt aatcgccgcg ggcgtggaga taactaacgc acacgggcag
    18001 ccaatcagca gcagcgtcag gcctttataa atccactcct gccagctggc ggcaaacagc
    18061 agcggtggca ccagcgtcac cagcagggcg acggccataa tcgctggcgt ataaatacgg
    18121 ctgaaacggt cgataaaccg ctcaatgggg gcgcggcgct cttcggcttc ttcgatcagt
    18181 ttcagaatcc ggtcaatggc gctggcaccc ggttctgaca gcacttccag cgttaccaga
    18241 cggtctacgc tggtggctcc tgcaggaact ttatcgcccg tcgcgcgctc caccggaatg
    18301 gattctccgg tcagggcgct ttcatcaaaa ctggcaaacg gtgagagcag tttaccgtcg
    18361 gcaggcaaac gcccacctgc ggcgacttca atcacatcgc caggccgcag gctgttaatc
    18421 gccacctctt cccgctcacc gttacgcagg cgcgtggcgg tttctggttt cagcgccatt
    18481 aacgcgctaa cgccctgacg cgcgcggctg gcggcccagc cttccagtcg ttcaccaatc
    18541 aaaaacagca gcaacaccat cgcagcttca gccgttgcgc caataaacag tgcaccaata
    18601 gcggctacgc tcattaaggt ttcaatggcg aagtagctgc cggatttgat cagccgtaat
    18661 gcctgatgag caatcgggta cagcccaacc agcgtggtcg cgataaacgc cagttgcccg
    18721 aacggatgat tgaactgctc cagaccccag ctgattgcca tcatcacgat tagtgtaatc
    18781 agcggcagat tctctttcag gcgcgatgct tgtggctcgt cggcggcctg cccatcgcgc
    18841 agggaatagc ccgctttttg caccgcagat tcaacttgtg cacgaatgtc attgtcggca
    18901 tcaaccacca gtttttcggt ggcgaacaac acctgcacct gattcacacc tgcaagctgg
    18961 cgcacggcat tttctacctt gcgcgcacag gtggcgcagt ccatgccact gactttccag
    19021 ctatagcggg tgccggagac gttttcagag agcgttggcg agctggagca tgcgccgtcg
    19081 cagcaacagt cgttggcgtt ctgtaccgtg gttagcggtt tgaacgcagc aaattgaggg
    19141 gctttcttgc cgtgattgtc aggtgtcgac atggcatact ccggttaagt tttttctcat
    19201 taaccgaagg atacactctg gagtcgactc cagagtcaag ttttatcaga gatacagtga
    19261 gcggacgatg aggaagtgac cggcaaagta gcaggccgca gcaatcgcgt tatccgcacg
    19321 gaagcgtcgg cgatagtggc tccccagcca gacaaagtta ctgataaaca gcaacgaagc
    19381 gccaacaaac gcagagagcg ccggagcggt cggacggaag aaccacagtt cacctgccag
    19441 ccacaccatc accagcgtca tgccgataaa cgtgcagata ggccaacgat actcttccag
    19501 ccgcgtccag ataatcgcca gtaacagcgc acccagaacc agcaacacca gtggcagagg
    19561 ccagaagaaa gagagcgtca tctgactggc gaaatagatg gtgtacagca ggtgcgagag
    19621 gaaaaacgcg ccgatggcgt acatcagacg ttgacgtggc aacagggtta gcgcatcgcc
    19681 cagcagtgag gcgcacagcc ctgccagcac cagatagcta atggcgtcga acatcggcgc
    19741 ttgccaggcc agtaacagca ggagaagaag ggttaacggt ttaaacaccc agcgttgcca
    19801 ggttggccca cgatacgatg catccacaga tagccatgcg gaaagacaga cagcgataaa
    19861 cgaccaaagc atcttagttc cttgaattcg ttattttttc gtaagcggaa tgcctacggt
    19921 cttgcttcca gtttagtggc gagggtggac ggatgacaac accataactg gcagggtatg
    19981 cttatttccg cattttccga tgagggataa agatgagcaa accacctctt ttctttattg
    20041 ttatcattgg cttaattgtc gtcgccgcat cgtttcgttt tatgcaacag cgacgggaaa
    20101 aagctgataa tgatatggct ccgctccagc aaaagctggt ggtggtgagc aacaagcggg
    20161 aaaaaccgat taacgatcgc cgttcgcgcc agcaggaagt gactccggca ggcaccagta
    20221 tgcgctatga ggcaagcttc aaaccgcaaa gcggaggaat ggagcagacg tttcgcctcg
    20281 acgcccagca gtaccatgcc ctgacagtgg gcgataaagg tacgctgagc tataaaggaa
    20341 cgcgctgtgt tagctttgtt ggcgaacaat aaggttattt tttaacttta aatttcttct
    20401 gccagaccag cagttcaaac acgccgaaaa ggaaaatccg caccttttcg gcggtggtca
    20461 tctgcgggcc atctttcggt aaagtggatt tcaatagcgc cagctgcata ccatgcatca
    20521 gcacggtaaa aatcagcgcc acgttaacga aaatattcag cgggcgcggg aagggatgca
    20581 ccagattgag gattaaaaat ccccaaacgc agagcattaa caaacgacca atattaatca
    20641 gcatcacttt ctccttgtgc ttcgcgttga tacagccgat aagccacctg acccgccact
    20701 ttttcccgat gtaatgacca gtttgccgga acagtgggca gaccgttttc gacttcgctt
    20761 tcgacataaa tcagggcttc gtcagccagc cagccgttat cttccagtaa atttatcgtc
    20821 tcttctaaca agccacggcg gaacggtgga tcgacaaaca cgatattatg cggtgtacct
    20881 ttttgcgcca ggaatgacat cgcgttgctg ttcaccacgc gtgcattgcc tgcttttagt
    20941 gtcgccagat tcttaattaa ctgctgagaa accgcgcgat ccatctcaat caacgtggcc
    21001 cccgcagcgt agcgcgataa cgcttccagt cccagcgcgc cgctcccggc gaagcaatcc
    21061 agacattggg cgtcaacaat gaccggagcc agccagttaa acaacgtttc gcgtacgcgg
    21121 tcggtggtgg ggcgcagacc tgggctatca ggaaccggga gtttacggcc tcgccactgc
    21181 ccgccaataa tgcggatttg gccgctgccg gaatgattcg gttttttcat gataaattgc
    21241 tcaatccgcc agataacaca tacttgcagg cggtgatgtg aattaagtta agtgatagac
    21301 tatttcatca tttttttagc tgctatgtac atagcgttaa cgctgtgcca tgaagcaaca
    21361 gcgaggagtg tagtcgcaaa tggcgaaaga aaaaaaacgt ggcttttttt cctggctggg
    21421 ctttggtcaa aaagagcaga ccccggaaaa agagacagaa gttcagaatg aacaaccggt
    21481 tgtagaagaa atcgttcagg cgcaagagcc tgcgaaggct tctgaacaag ccgtcgaaga
    21541 gcaaccgcag gcgcatactg aagccgaggc ggaaactttt gctgccgacg ttgtggaagt
    21601 cactgaacag gttgctgaaa gtgaaaaagc gcagcctgaa gcggaagtcg ttgcacagcc
    21661 ggaaccggtc gtagaagaaa cgccggagcc agtggctatc gaacgtgaag agctgccgtt
    21721 gccggaagac gtcaacgccg aagaggtttc gccagaagag tggcaggctg aagcggaaac
    21781 cgtagagatt gtcgaagcgg cggaagaaga agcggctaaa gaagaaatca ccgacgaaga
    21841 gctggaagca caggcgctgg ctgccgaagc ggcagaagag gcagaagagg cggtgatggt
    21901 ggttcctccg gtagaagagc agccggtgga agaaatcgct caggagcagg aaaaaccgac
    21961 caaagaaggt ttcttcgcgc gcctgaaacg cagcctgtta aaaaccaaag aaaatctcgg
    22021 ttccggattt atcagcctgt tccgcggtaa aaaaatcgac gatgatctgt ttgaggagct
    22081 ggaagaacag ctgttgatcg ccgatgtggg cgtggaaacc acacgtaaaa ttatcaccaa
    22141 tctgacggaa ggcgcatccc gcaagcagct tcgtgatgcc gaggcgctct atggcctgct
    22201 gaaagaagag atgggcgaga ttctggcgaa agtcgatgag ccgctgaatg ttgaaggtaa
    22261 aacgccgttc gtgatcctga tggtgggcgt caacggtgtg ggtaaaacta cgacgattgg
    22321 taagctggca cgtcagtttg agctgcaggg taaatcggtg atgctggcgg cgggcgatac
    22381 tttccgtgca gcagcggttg aacagcttca ggtctggggt cagcgcaaca atattccggt
    22441 gattgcccag cataccggtg cggattccgc ctctgttatc ttcgacgcca ttcaggcagc
    22501 taaagcgcgt aatatcgacg tcctgattgc cgataccgcc ggacgcctgc agaacaaatc
    22561 gcacctgatg gaagagttga agaaaatcgt ccgcgtgatg aagaaactcg acgttgaagc
    22621 gccgcatgaa gttatgctga ctattgatgc cagtaccggg cagaatgcgg taagccaggc
    22681 caaactgttc cacgaagccg ttggcttaac cggcatcacg ctaacgaaac tggacggcac
    22741 ggcgaaaggc ggggtaattt tctcggtggc tgaccagttt ggtatcccta tccgctacat
    22801 tggtgtcggc gaacgtattg aggatttgcg tccgtttaag gcggacgact ttatagaggc
    22861 actttttgcc cgagaggatt aacaatgatt cgctttgaac atgtcagcaa ggcttatctc
    22921 ggtgggagac aggcgctgca gggcgttacg ttccatatgc agccgggtga gatggcgttt
    22981 ctgaccggtc attccggcgc agggaaaagt accctcctga agctgatctg tgggattgag
    23041 cggcccagcg ccgggaaaat ctggtttagc ggccatgaca tcacgcgtct gaaaaaccgt
    23101 gaagttccgt ttctgcgccg ccagattggc atgattttcc aggatcacca tctactgatg
    23161 gaccgtactg tctacgataa cgtggcgatc ccgctgatta tcgctggtgc cagcggtgac
    23221 gatattcgtc gccgggtgtc ggcggcactg gataaagtcg ggctattgga caaagcgaag
    23281 aacttcccta ttcagctttc cggcggtgaa caacagcgtg ttggcattgc ccgcgcggtg
    23341 gtgaataagc ccgcggtact gctggcggat gaaccgaccg gtaacctaga cgacgcgttg
    23401 tcggaaggga ttctacgtct gtttgaagag tttaaccgcg ttggggtaac cgtattgatg
    23461 gcaacgcacg acatcaacct gatttcacgg cgttcctatc gcatgctcac cctgagcgat
    23521 ggtcacttgc atggaggcgt gggccatgaa taagcgcgat gcaatcaatc atattcggca
    23581 gtttggtggg cgtctggatc gcttccgtaa atcggtcggc ggctcaggcg acggcggtcg
    23641 taacgcaccg aaacgcgtga aatcctcgcc aaaaccggta aatcgcaaaa ccaacgtttt
    23701 caacgaacag gtgcgctatg ccttccacgg cgcattgcag gatctgaaaa gcaaaccgtt
    23761 cgccacgttt ttaacggtga tggttatcgc catctccctg acgctgccca gcgtctgtta
    23821 tatggtgtac aaaaacgtta atcaggcggc gacgcagtat tatccgtcac cgcaaatcac
    23881 tgtttacttg caaaaaacgc tggacgatga cgccgctgcg ggcgtggtgg cacagttgca
    23941 ggccgagcaa ggcgtggaga aagtgaacta tctttctcgt gaagacgcac tgggcgagtt
    24001 ccgtaactgg tctggttttg gtggtgcgct ggatatgctg gaagaaaacc cgcttccggc
    24061 agtggcggtg gtgatcccga aactcgattt tcaggggacg gaatcactca atacgctgcg
    24121 tgatcgtatc acgcagatta acggcattga cgaagtgcgg atggatgaca gctggtttgc
    24181 ccgtctggcg gcgttgaccg ggctggtcgg gcgcgtttcg gcgatgatcg gcgtgttgat
    24241 ggtggcggca gtgttcctcg tcatcggcaa cagcgtacgt ctgagcatct ttgctcgccg
    24301 tgactccatc aacgtgcaga aactgattgg tgcgacggat ggtttcatcc ttcgcccgtt
    24361 cctgtatggt ggcgcactgc tgggattttc tggcgcattg ttgtcattaa ttttgtcaga
    24421 aattctggtg ctgcgattgt catcggcggt tgcggaagtg gcacaggttt tcggaacgaa
    24481 ttttgatatc aatggtttat cgttcgatga atgcctgcta ttgctgctgg tatgctcgat
    24541 gattggctgg gtggcagcgt ggcttgccac ggtacaacat ttacgccact ttacgcctga
    24601 ataataaaag cgtgttatac tctttccctg caatgggttc cgtagcaggg aaagagatcc
    24661 cgttgtctct tcccggtatt tcatctctat gtcacatttt gtgcgtaatt tattcacaag
    24721 cttgcattga acttgtggat aaaatcacgg tctgataaaa cagtgaatga taacctcgtt
    24781 gctcttaagc tctggcacag ttgttgctac cactgaagcg ccagaagata tcgattgaga
    24841 ggatttgaat gactgacaaa atgcaaagtt tagctttagc cccagttggc aacctggatt
    24901 cctacatccg ggcagctaac gcgtggccga tgttgtcggc tgacgaggag cgggcgctgg
    24961 ctgaaaagct gcattaccat ggcgatctgg aagcagctaa aacgctgatc ctgtctcacc
    25021 tgcggtttgt tgttcatatt gctcgtaatt atgcgggcta tggcctgcca caggcggatt
    25081 tgattcagga aggtaacatc ggcctgatga aagcagtgcg ccgtttcaac ccggaagtgg
    25141 gtgtgcgcct ggtctccttc gccgttcact ggatcaaagc agagatccac gaatacgttc
    25201 tgcgtaactg gcgtatcgtc aaagttgcga ccaccaaagc gcagcgcaaa ctgttcttca
    25261 acctgcgtaa aactaagcag cgtctgggct ggtttaacca ggatgaagtc gaaatggtgg
    25321 cccgtgaact gggcgtaacc agcaaagacg ttcgtgagat ggaatcacgt atggcggcac
    25381 aggacatgac ctttgacctg tcttccgacg acgattccga cagccagccg atggcaccgg
    25441 tgctctatct gcaggataaa tcatctaact ttgccgacgg catcgaagat gataactggg
    25501 aagagcaggc ggcaaaccgt ctgaccgacg cgatgcaagg tctggacgaa cgcagccagg
    25561 acatcatccg cgcgcgctgg ctggacgaag acaacaagtc cacgttgcag gaactggctg
    25621 accgttacgg tgtttccgct gaacgtgtac gccagctgga aaagaacgcg atgaaaaaac
    25681 tgcgcgctgc tatagaagcg taatttccgc tattaagcag agaaccctgg atgagagtcc
    25741 ggggtttttg ttttttgggc ctctacaata atcaattccc cctccggcaa aacgtcaatc
    25801 cccacgcaga ttgttaataa actgtcaaaa tagctattcc aatatcataa aaatggggta
    25861 tgttttagca gagtatgctg ctaaagcacg ggtagtcatg cataaaacga aataaagtgc
    25921 tgaatcacaa caccacaaca cacgtaataa ccagaagaat ggggattctc aggatgaata
    25981 taaagggtaa agcgttactg gcaggatgta tcgcgctggc attcagcaat atggctctgg
    26041 cagaagatat taaagtcgcg gtcgtgggcg caatgtccgg tccggttgcg cagtacggtg
    26101 accaggagtt taccggcgca gagcaggcgg ttgcggatat caacgctaaa ggcggcatta
    26161 aaggcaacaa actgcaaatc gtaaaatatg acgatgcctg tgacccgaaa caggcggttg
    26221 cggtggcgaa caaagtcgtt aacgacggca ttaaatatgt gattggtcac ctctgttctt
    26281 catcaacgca gcctgcgtct gatatctacg aagacgaagg cattttaatg atcaccccag
    26341 cggcaaccgc gccggagctg accgcccgtg gctatcagct gatcctgcgc accaccggcc
    26401 tggactccga ccaggggcca acggcggcga aatatattct tgagaaagtg aaaccgcagc
    26461 gtattgctat cgttcacgac aaacagcaat acggcgaagg tctggcgcga gcggtgcagg
    26521 acggcctgaa gaaaggcaat gctaacgtgg tgttctttga tggtatcacc gccggggaaa
    26581 aagatttctc aacgctggtg gcgcgtctga aaaaagagaa tatcgacttc gtttactacg
    26641 gcggttatca cccggaaatg gggcaaatcc tgcgtcaggc acgcgcggca gggctgaaaa
    26701 ctcagtttat ggggccggaa ggtgtggcta acgtttcgct gtctaacatt gcgggcgaat
    26761 cagcggaagg gctgctggtg accaagccga agaactacga tcaggttccg gcgaacaaac
    26821 ccattgttga cgccatcaaa gcgaaaaaac aggacccaag cggcgcattc gtttggacca
    26881 cctacgccgc gctgcaatct ttgcaggcgg gcctgaatca gtctgacgat ccggctgaga
    26941 tcgccaaata cctgaaagcg aactccgtgg ataccgtgat gggtccgctg acgtgggatg
    27001 agaaaggtga tctgaaaggc tttgagttcg gcgtatttga ctggcacgcc aacggcacgg
    27061 cgaccgatgc gaagtaatca ttaatcggca actttgggtt gccgccaaat tgctaatatc
    27121 gagtacgttg cttcatgccg gatgcggcgt aaacgcctta tccggcctac aagatccaaa
    27181 gaaatcagta aattgcaaca cacattgtag gcctgataag cgtagcgcat caggcaattt
    27241 ctcgttggtc atcacttttg aaatcaactt gacgattaat gcttctccca gccgccctgt
    27301 tgcgccgtaa atcccagtgc ctgcataaac gccgtcatca caccgcgatc ttccacgcct
    27361 gcatccgcca tccaccagca tgaaacgcca ggattgttac gcaaaacctc ttccagcaga
    27421 tattgcccca caccgcgacg tcgggtgact tcccgcacac gcagggaatc cagtgctccc
    27481 tcggtgccgc ttaagattac ccgtacggca gcgagcaggc gctcgttaaa acgcgcggcg
    27541 tagatacggt ggttatcgtc aacctgtaaa gaggaagggg aatactccgg ccagatcttt
    27601 tgcaggtcaa tccggtcctg gtcgctaaag ttttctaatc gaatgatggt cagcttcatg
    27661 ggtaacccgt gtaaatcaca aaagtggaac cagtgtagcg aaataattta atcggaggct
    27721 ttctcttttt tatttctttt ggcaggtgat taatttttta acagcaataa ttacaaagtt
    27781 aaaacattag agaatgaaaa atacccagca taatcccctg aatggtagtg aattattccg
    27841 ccctttgtgc cgttatttta tgctgacaaa ggcacttttt tctgtttatc tatcaataaa
    27901 ttcagaatat tatctgttct taatcgactg aaagataggg attttaatca ctattatcac
    27961 aaaatactgc gctaaaccct taatcagaca ggcaaaaaca gtgcagtata aaaaaagaac
    28021 agtctgattt gttaacacat aaaaacaaag caacacaaca tcacgaatgg ggatttttga
    28081 ctatgaaacg gaatgcgaaa actatcatcg cagggatgat tgcactggca atttcacaca
    28141 ccgctatggc tgacgatatt aaagtcgccg ttgtcggcgc gatgtccggc ccgattgccc
    28201 agtggggcga tatggaattt aacggcgcgc gtcaggcgat taaagacatt aatgccaaag
    28261 ggggaattaa gggcgacaag ctggttggcg tggaatatga cgacgcctgc gacccgaaac
    28321 aagccgttgc ggtcgccaac aaaatcgtta atgacggcat taaatacgtt attggtcatc
    28381 tgtgttcttc ttctacccaa cctgcatcag atatctacga agacgaaggt attctgatga
    28441 tctcaccggg agctaccaac ccggagctga cccaacgcgg ttatcaacac attatgcgta
    28501 ctgccgggct ggactcttcc caggggccaa cggcggcaaa atacattctt gagacggtga
    28561 agccccagcg catcgccatc attcacgaca aacaacagta tggcgaaggg ctggcgcgtt
    28621 cggtgcagga cgggctgaaa gcggctaacg ccaacgtcgt cttcttcgac ggtattaccg
    28681 ccggggagaa agatttctcc gcgctgatcg cccgcctgaa aaaagaaaac atcgacttcg
    28741 tttactacgg cggttactac ccggaaatgg ggcagatgct gcgccaggcc cgttccgttg
    28801 gcctgaaaac tcagtttatg gggccggaag gtgtgggtaa cgcatcattg tcgaatattg
    28861 ccggtgatgc tgccgaaggc atgttggtca ctatgccaaa acgctatgac caggatccgg
    28921 caaaccaggg catcgttgat gcgctgaaag cagacaagaa agatccgtcc gggccttatg
    28981 tctggatcac ctacgcggcg gtgcaatctc tggcgactgc ccttgagcgt actggcagcg
    29041 atgagccgct ggcgctggtg aaagatttaa aagctaacgg tgcaaacacc gtgattgggc
    29101 cgctgaactg ggatgaaaaa ggcgatctta agggatttga ttttggtgtc ttccagtggc
    29161 acgccgacgg ttcatccacg gcagccaagt gatcatccca ccgcccgtaa aatgcgggcg
    29221 ggtttagaaa ggttacctta tgtctgagca gtttttgtat ttcttgcagc agatgtttaa
    29281 cggcgtcacg ctgggcagta cctacgcgct gatagccatc ggctacacca tggtttacgg
    29341 cattatcggc atgatcaact tcgcccacgg cgaggtttat atgattggca gctacgtctc
    29401 atttatgatc atcgccgcgc tgatgatgat gggcattgat accggctggc tgctggtggc
    29461 ggcgggattc gtcggcgcaa tcgtcattgc cagcgcctac ggctggagta tcgaacgggt
    29521 agcctatcgc ccggtgcgta actctaagcg cctgattgcg ctcatctcgg caatcggcat
    29581 gtccatcttc ctgcaaaact acgttagcct gactgaaggt tcgcgtgacg tggcgctgcc
    29641 cagtctgttt aacggtcagt gggtggtggg gcatagcgaa aacttctctg cctctattac
    29701 caccatgcag gcagtgatct ggattgttac tttcctcgcc atgctggcgc tgacgatttt
    29761 cattcgttat tcccgtatgg ggcgcgcctg ccgtgcctgc gcggaagatc tgaaaatggc
    29821 gagcctgctt ggcatcaaca ccgaccgggt gattgctttg acctttgtta ttggcgcggc
    29881 gatggcggca gtggctggcg tactgctcgg tcagttctac ggcgtcatta acccctatat
    29941 cggctttatg gccgggatga aagcctttac cgctgcggtg ctcggtggga ttggcagcat
    30001 tccaggggcg atgattggtg ggctgatcct ggggattgcg gaggcgctct cttctgccta
    30061 tctgagtacg gaatataagg atgtggtttc atttgccctg ctgattctgg tgctgctggt
    30121 gatgccgacc ggtattctgg gtcgcccgga ggtagagaaa gtatgaaacc gatgcatatt
    30181 gcaatggcgc tgctctctgc cgcgatgttt tttgttctgg cgggcgtctt tatgggcgtg
    30241 caactggagc tggatggcac caaactggtg gtcgacacgg cttcagacat ccgttggcag
    30301 tgggtgttta tcggcacggc ggtggtcttt ttcttccagc ttttgcgacc ggctttccag
    30361 aaagggctaa aaagcgtttc cggaccgaag tttattctgc ccgcgattga tggctccacg
    30421 gtgaagcaga aactgttcct cgtggcgctg ttggtgcttg cggtggcgtg gccgtttatg
    30481 gtgtcacgtg ggacggtgga tatcgccact ctgaccatga tctacattat cctcggtctc
    30541 gggctgaatg tggttgttgg tctttctggt ctgctggtgc tggggtacgg cggtttttac
    30601 gccatcggcg cttacacctt tgcgctgctc aatcactatt acggcttggg cttctggact
    30661 tgtctgccga ttgccgggtt aatggcagcg gcggcgggct tcctgctcgg ctttccggtg
    30721 ctgcgtctgc gcggtgatta tctggcgatc gttaccctcg gcttcggcga aattgtgcgc
    30781 atattgctgc tcaataacac cgaaattacc ggcggcccga acggaatcag tcagatcccg
    30841 aaaccgactt tcttcggcct tgagttcagc cgtacagccc gtgaaggcgg ttgggacacg
    30901 ttcagtaatt tctttggcct gaaatacgat ccctccgatc gcgtcatctt cctgtatctg
    30961 gtggcgttgc tgctggtggt gctaagcctg tttgtcatta accgcctgct gcggatgccg
    31021 ctggggcgtg cgtgggaagc gttgcgtgaa gatgagatcg cctgccgttc gctgggctta
    31081 agcccgcgtc gtatcaagct gaccgctttt accatcagtg ccgcgtttgc cggttttgcc
    31141 ggaacgctgt ttgctgcgcg ccagggcttt gtcagcccgg aatccttcac ctttgctgaa
    31201 tcggcgtttg tactggcgat agtggtgctc ggcggtatgg gatcgcagtt tgcggtgatt
    31261 ctggcggcaa ttttgctggt ggtgtcgcgc gagttgatgc gtgatttcaa cgaatacagc
    31321 atgttaatgc tcggtggttt gatggtgctg atgatgatct ggcgtccgca gggcttgctg
    31381 cccatgacgc gcccgcaact gaagctgaaa aacggcgcag cgaaaggaga gcaggcatga
    31441 gtcagccatt attatctgtg aacggcctga tgatgcgctt cggcggcctg ctggcggtga
    31501 acaacgtcaa tcttgaactg tacccgcagg agatcgtctc gttaatcggt cctaacggtg
    31561 ccggaaaaac cacagtcttt aactgcctga ccggattcta caaacccact ggaggcacca
    31621 ttttactgcg cgatcagcat ctggaaggtt taccggggca gcaaattgcc cgcatgggcg
    31681 tggtgcgtac cttccagcat gtgcgtctgt tccgtgaaat gacggtgatt gaaaacctgc
    31741 tggtggcgca gcatcagcaa ctgaaaaccg ggctgttctc tggcctgttg aaaacgccat
    31801 ccttccgtcg cgcccagagc gaagcgctcg atcgcgccgc gacctggctt gagcgcattg
    31861 gtttgctgga acacgccaac cgtcaggcga gtaacctggc ctatggtgac cagcgccgtc
    31921 ttgagattgc ccgctgcatg gtgacgcagc cggagatttt aatgctcgac gaacctgcgg
    31981 caggtcttaa cccgaaagag acgaaagagc tggatgagct gattgccgaa ctgcgcaatc
    32041 atcacaacac cactatcttg ttgattgaac acgatatgaa gctggtgatg ggaatttcgg
    32101 accgaattta cgtggtcaat caggggacgc cactggcaaa cggtacgccg gagcaaatcc
    32161 gtaataaccc ggacgtgatc cgtgcctatt taggtgaggc ataagatgga aaaagtcatg
    32221 ttgtcctttg acaaagtcag cgcccactac ggcaaaatcc aggcgctgca tgaggtgagc
    32281 ctgcatatca atcagggcga gattgtcacg ctgattggcg cgaacggggc ggggaaaacc
    32341 accttgctcg gcacgttatg cggcgatccg cgcgccgcca gcgggcgaat tgtgtttgat
    32401 gataaagaca ttaccgactg gcagacggcg aaaatcatgc gcgaagcggt agcgattgtc
    32461 ccggaagggc gtcgcgtctt ctcgcggatg acggtggaag agaacctggc gatgggcggc
    32521 ttttttgccg aacgcgacca gttccaggag cgcataaagt gggtgtatga actgtttcca
    32581 cgtctgcatg agcgccgtgt tcagcgggca ggcaccatgt ccggcggtga gcaacaaatg
    32641 ctggcgattg gtcgcgcgct gatgagcaac ccgcgtttgc tgctacttga tgagccatcg
    32701 ctcggtcttg cgccgattat catccagcaa attttcgaca ccatcgagca gctgcgcgag
    32761 caggggatga ctatcttcct cgtcgagcag aacgccaacc aggcgctaaa gctggcggat
    32821 cgcggctacg tgctggaaaa cggccatgtg gtgctctccg ataccggcga cgcgctgctg
    32881 gcgaacgaag cagtgagaag tgcgtattta ggcgggtaat aacacgttga ttgataagga
    32941 gtcaaaagac tcctttgaga caggtgacaa atgtaaaatt gcctgatgcg ctgcgcttat
    33001 caggcctact gggtgagttg caatatattg aatttgcacg atcttgtagg ccggataagg
    33061 cgtttacgcc gcatccggca tgaaacgatg agtaatctgt agagtttgat tcagaccttc
    33121 tatttttccg cttatccgtg ccccatctcc cattttccct cacccacgcc gtcaccgcct
    33181 tgtcatcttt ctgacacctt actatcttac aaatgtaaca aaaaagttat ttttctgtaa
    33241 ttcgagcatg tcatgttacc ccgcgagcat aaaacgcgtg aattcgcgca ttcggtacaa
    33301 caagagagat aaacgatgaa accgttacat tatacagctt cagcactggc gctcggactg
    33361 gcgttaatgg ggaatgcaca ggcagtgacg accattccgt tctggcattc tatggaaggg
    33421 gaactgggta aagaggtgga ttctctggcc caacgtttta acgccgaaaa tccggattac
    33481 aaaattgtac cgacctataa aggcaactac gaacagaatt taagcgcggg gattgccgca
    33541 tttcgtaccg gcaacgctcc ggctattttg caggtttatg aagttggcac cgccaccatg
    33601 atggcatcga aagccattaa accggtaata tgacgtgttt aaagaggcgg ggattcaatt
    33661 cgatgagtcg cagtttgtgc cgacggtttc cggttactac tccgacagca aaacgggcca
    33721 cttactctcc cagccattca acagctcgac tcccgttctc tattacaaca aagacgcctt
    33781 caagaaagcg gggttagacc cggcacagcc gccgaaaacc tggcaggatc tggcggacta
    33841 tgccgcgaaa ctgaaagcct ccggcatgaa gtgcggctac gccagcggct ggcagggctg
    33901 gatccaactg gaaaacttta gcgcttggaa cggtctgccg tttgccagca aaaacaacgg
    33961 ctttgacggc acagatgcgg tgctggagtt caacaagccg gagcaggtga aacacatcgc
    34021 catgctcgag gagatgaaca agaagggcga cttcagctac gtcggtcgta aggatgaatc
    34081 caccgagaag ttctataacg gtgattgcgc gatgaccacc gcctcttccg gttctcttgc
    34141 caacattcgc gagtacgcca aatttaacta cggcgtaggc atgatgcctt acgacgccga
    34201 tgcgaaagat gcgccacaaa acgccattat cggcggagcc agcctgtggg tgatgcaggg
    34261 taaagataaa gaaacgtata ccggtgcggc gaagttcctc gatttcctcg cgaagccaga
    34321 aaacgctgcc gagtggcatc agaaaaccgg ttatctgcca atcaccaaag cagcgtatga
    34381 cctgacccgt gagcagggct tttatgagaa aaacccaggg gcggataccg cgacgcgtca
    34441 gatgctgaat aagccgccgt tgccgttcac caaagggctg cgtctgggca acatgccgca
    34501 gatccgcgtg attgtggatg aagagctgga gagcgtgtgg accggtaaga agacaccaca
    34561 gcaggcactg gataccgccg ttgagcgtgg gaaccagtta ctgcgccgct ttgagaaatc
    34621 gacgaagtct taatcagtgt aatgtcggat gcgtttcgct tatctgacct ggcatcgcgt
    34681 gtaggccgga taagcgaagc gcatccggca cagttcagga attaaccgta atgtcatcat
    34741 cccgtccggt gttccgctcg cgctggctgc cttatctgct ggtcgcgccg cagctcatca
    34801 tcaccgttat cttttttatc tggcctgcgg gcgaagcgtt gtggtactcg ctgcaaagcg
    34861 tcgatccgtt tggtttctcc agccagtttg tcggtctgga taacttcgtc acgctgttcc
    34921 atgacagcta ctatctagac tccttctgga cgacgataaa attcagcacc tttgtcaccg
    34981 tcagcggttt gctggtatcg ctgttctttg cggcgctggt ggagtacatc gtgcgcggca
    35041 gccgtttcta tcaaaccttg atgctgctgc cgtatgccgt ggctcccgcc gttgccgccg
    35101 tattgtggat cttcctgttt aaccccggtc gcgggctgat cacccatttt ctcgctgagt
    35161 tcggctacga ctggaaccac gcgcaaaaca gcggtcaggc aatgtttctg gtggtgtttg
    35221 cctcggtgtg gaagcaaatc agctacaact ttctgttctt ctatgccgcg ctgcactcca
    35281 ttccccgttc gttgatcgaa gccgcagcca tcgacggtgc agggccgatt cgccgcttct
    35341 ttaagattgc gctgccgctt atcgccccgg tgagtttctt cctgctggta gtgaacctgg
    35401 tgtatgcctt cttcgacacc ttcccggtga tcgacgccgc cacgtccggc gggccggttc
    35461 aggccaccac gacgctgatt tataagatct accgcgaagg ttttaccgga ctggatctgg
    35521 cttcgtctgc cgcacagtcg gtggtgttga tgttcctcgt catcgtgctg acggtggtgc
    35581 agttccgcta tgttgaaagc aaggtgcgtt accaatgatt gagaaccgtc cgtggctgac
    35641 gatattcagc cataccatgc tgatcctcgg gatcgcggtg atcctcttcc cgctgtacgt
    35701 ggcgtttgtc gcggcgacgc tggataaaca ggccgtctat gccgcgccga tgacgctcat
    35761 ccccggcaca catctgctgg aaaatatcca caacatctgg gttaacgggg taggtacgaa
    35821 tagcgcgccg ttctggcgga tgttgcttaa cagctttgtg atggcgttca gcattacgct
    35881 cggcaaaatt accgtctcga tgctctcggc atttgccatt gtctggtttc gttttccgct
    35941 acgtaacctc ttcttctgga tgatttttat caccctgatg ctgccggttg aagtacgtat
    36001 cttcccgacg gtggaagtca tcgccaacct gaagatgctc gacagctacg ccggtttaac
    36061 gctgccgctg atggcctcgg cgaccgccac gtttctgttt cgccagttct ttatgacgct
    36121 gccggatgaa ctggtggaag cggcgtggat cgacggcgca tcgccaatgc gcttcttttg
    36181 cgacatcgtt tttccgctct ccaaaactaa tctggcggcg ctgtttgtga tcacctttat
    36241 ttacggctgg aaccagtatt tgtggccgtt gttgattatt accgacgtgg atctcggcac
    36301 caccgtggca gggatcaaag ggatgatcgc cactggcgaa ggcaccacgg aatggaactc
    36361 agtgatggcg gcgatgttgt taaccctgat cccaccagta gtgattgttt tagtgatgca
    36421 gcgtgccttc gtgcgcggcc tggtcgatag tgagaaataa gatggcagga ctgaaattac
    36481 aggcagtaac caaaagctgg gatggcaaaa cccaggtcat taaaccgctg acccttgatg
    36541 tggctgatgg cgaatttatc gtgatggtcg ggccgtctgg ctgcgggaaa tcgacgctgc
    36601 tgcgtatggt tgccgggctg gagcgggtga ccgaaggcga tatctggatc aacgaccagc
    36661 gcgtgactga aatggagccg aaagatcgcg ggattgcgat ggtgttccag aactacgcgc
    36721 tttatccgca tatgagcgtc gaagaaaaca tggcgtgggg gcttaaaatt cgcggcatgg
    36781 gcaagcagca aattgccgag cgcgttaaag aagtggagct ggacggtctg ctcaaacgtc
    36841 gcccgcgtga gctatccggc ggtcagcgtc agcgtgtagc gatgggccgc gcgattgtgc
    36901 gcgatccggc ggtgttcctg tttgatgagc cgctctctaa cctcgatgcc aagctgcgcg
    36961 tgcagatgcg tcttgaactg caacagttgc accgtcgcct gaaaacgact tcactctacg
    37021 ttactcacga tcaggttgaa gcgatgacgc tcgcccagcg agtaatggtg atgaacggcg
    37081 gcgttgccga acagattggc acaccagttg aagtctacga aaagcccgcc agcctgtttg
    37141 tagcgagttt tatcggcagt ccggcgatga acctgctgac aggccgcgtg aataacgaag
    37201 gcacgcattt cgaactggac ggcggtattg agctgccgct aaacggtggc taccgtcagt
    37261 atgccgggcg taaaatgact ctcggcattc gcccggaaca tattgtgtta agctcgcagg
    37321 cagaaggcgg cgtaccgctg gtgatggaca cgctggagat cctcggcgca gataacctgg
    37381 cgcacggacg ctggggcgag cagaagctgg tggtgcgact ggcgcatcag gagcgcccga
    37441 cggcaggcag cacgctgtgg ctgcatctgg cggaaaatca gctgcatctt tttgatggtg
    37501 aaacaggaca acgagtatga gtaactggcc ttatccccgc atcgtcgctc atcgtggcgg
    37561 cggtaagctg gccccggaaa acaccctggc ggcaatcgac gtcggggcaa aatacggtca
    37621 taagatgatc gaatttgacg cgaagttatc gaaagatggc gagatcttcc tgctccatga
    37681 cgacaatctc gaacgcacca gcaacggctg gggcgtcgcg ggtgaactga actggcagga
    37741 tttactgcgc gtggatgcgg gcagttggta cagcaaagcg tttaaaggtg agccgctgcc
    37801 gttgctttcg caggtggcgg aacgctgtcg cgaacacggg atgatggcga atatcgaaat
    37861 caaacccacc actggcactg gaccattaac gggcaaaatg gtggcgctgg cggcacgcca
    37921 actgtgggcc ggtatgacgc cgccgctgct gtcatcgttt gagattgatg ctttagaagc
    37981 tgcacagctg gcggcaccgg aactgccgcg tggtttattg cttgatgagt ggcgcgacga
    38041 ctggcgcgaa ctgaccgcgc ggctgggctg cgtctctatt catctcaatc ataagttact
    38101 cgataaagcg cgagtgatgc agttgaaaga cgccggatta cggattctgg tttataccgt
    38161 caataaaccc cagcgcgcgg cagagttgct gcgctggggc gtggattgca tctgtaccga
    38221 tgcgattgac gtgattggtc cgaactttac ggcccaatag ttttcaacgg aatgtcaggc
    38281 tgcggcttgc tgggctgatt cagcatgtcg ccgtttctcc tctccggcag catatgctgc
    38341 tgattcaacg aactatccgg attacggttg ctgtttaaca tcccgccgtt ggtgttgggc
    38401 agcatttgct gccgggcggg atttcgctcc cccggctgtg actgcaacac ccgctgagaa
    38461 ttattgttta tctggttttc taaatgctgc tgttgcaact gagtttgcgt tttcagttgc
    38521 tgattcagca tccctttttg ctggatttgc tgagtttgca tctgggtttg catccgctgc
    38581 tggctgggta tctgataccc cggctggtta gggttgttca gagtattaat gggctgtgca
    38641 aagccgacaa acggcaggag tgccgtaaga atcagaagtc gtttcatcgc gtatcctcct
    38701 ctgaagatat cctttaagtt tactcgcttc ccgacaaaac gatgattaat tcagagttat
    38761 ataccaggct tagctggggt tgccccttaa tctctggaga ataacgatga taaaaccgac
    38821 gtttttacgc cgggtagcca ttgctgctct gctctcagga agttgtttta gcgccgtcgc
    38881 cgcgcctcct gtttcttacg gcgtggagga agatatcttc catccggtac gcgcgaaaca
    38941 ggggatggtg gcgtctgtcg atgccactgc cacccaagtg ggggtagata ttctaaagga
    39001 gggcgggaat gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg taacgcatcc
    39061 gcaggcaggg aatctgggcg gtggtggttt tatgctgatc cactcgaaaa atggcaatac
    39121 caccgctatc gatttccgcg agatggcacc cgctaaagcg acccgcgata tgttccttga
    39181 tgaccagggc aacccggaca gcaaaaaatc actcacttcg catctagctt ccggcacgcc
    39241 cggtacggtc gctggtttct cgctggcgtt ggataagtac ggcactatgc cgttgaacaa
    39301 agtggtgcag cccgcgttca aactggcacg cgatggtttt atcgttaacg atgctttggc
    39361 agacgatctc aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca gcaaagctat
    39421 cttctggaaa gagggcgaac cgctgaaaaa gggcgacaag ctggtgcagg cgaacctggc
    39481 aaagagcctg gagatgattg ccgaaaacgg cccggacgaa ttctataaag gcacgatagc
    39541 ggaacagatt gcccaggaga tgcagaaaaa cggtggcttg atcactaaag aagatttagc
    39601 ggcgtataaa gcggtcgaac gcacaccgat aagtggcgat tatcgcgggt atcaggttta
    39661 ctccatgcca ccgccatcct ccggcgggat ccatatcgta caaatcctca atattctgga
    39721 aaacttcgat atgaagaaat acggctttgg cagcgccgat gcgatgcaaa tcatggcaga
    39781 agcggagaaa tacgcctacg ccgaccgctc ggaatatctt ggcgacccgg attttgtcaa
    39841 agtaccgtgg caggcgctga ccaataaagc ctatgccaaa tctattgccg atcaaattga
    39901 tatcaataaa gcgaagccat ccagcgaaat tcgccccggc aagcttgcgc cttatgagag
    39961 caatcaaact acccattact cagtggtgga taaagatggt aacgcggtgg cggtgaccta
    40021 tacgctgaac accaccttcg gtacgggcat tgtcgcgggc gagagcggta ttctgcttaa
    40081 taatcagatg gatgatttct ccgctaaacc gggcgtaccg aacgtttacg ggctggtggg
    40141 cggtgatgcc aacgccgtcg ggccgaataa acgtccgctg tcgtcaatgt caccaacgat
    40201 tgtggtgaaa gacggtaaaa cctggctggt caccggtagc cctggcggga gccggatcat
    40261 cactacagtg ctgcaaatgg tggtgaatag catcgattat ggcatgaacg tcgccgaagc
    40321 gaccaatgcg ccgcgtttcc accatcagtg gttgccggac gagctgcgtg tcgaaaaagg
    40381 gtttagcccg gatacgctca agctgctgga agcaaaaggt cagaaagtgg cgctgaaaga
    40441 ggcgatgggc agtacacaaa gcattatggt tgggccggac ggtgagttgt acggcgcatc
    40501 cgacccgcgc tcggtggatg atttaacggc ggggtactaa ggttagcggc cctcttcgtg
    40561 ggaagagggc tattttgtca gggcaagccg aaggtagcct tttttatttc gtaatcctgt
    40621 agatattctt ccagcggctt tgccgttccc agagaatgaa tttcaccact gtctttatca
    40681 ataataaaag gtgcgttacc agctaagcgc gcggcctcat ctccagtttc gagaaattct
    40741 cgtgcttcga aacagaaata ccagccctgg ctaaagcgtc catgtagagt aatgacgacc
    40801 gggagatctg catcatcaag gtaatggttc gctttcgcga atgcgtcgtg ataagtaatc
    40861 ataattataa taaatattat tgttgagtgt tatattatta ttcctgtgtg atacattgag
    40921 caaagacgcg ttcatcttcg tcaaagatat tattaaatgc tggttttgaa aaaagttcat
    40981 cggataagat atagtcacga aataagtatc tgctaatatt ttatgatttc tttttcagaa
    41041 cgtcgaacgg gattttctta tttaagatat cagaatctta tgattcgggt aaaattctaa
    41101 ataaatcaac atgtcattaa aatcatcata gccattagct atttcggcta aaatagagac
    41161 tacatgtctt cggtccatct cacttaagga gtgtagttcc gttgtaagtt tttccatagc
    41221 ttgcactgct aaatttcgaa caaggaattt tctgctggta atctctaaaa agatggcatg
    41281 gtttacaatg atttttgttt ccttttgatt attatgaaca actgtccatg atttcgttta
    41341 agaatgaaga gaaatcacta aacgaactga atatattttc tgtgccaata ttatctctaa
    41401 tttcaaaaaa gttactttta atgtcataag taaatattga aatgctatca tccccgatca
    41461 ctaaccgttc ttgtagatca gggtttatga aatcatcatt atttctataa aattcattta
    41521 cggcaaaaag gtttttaact gaaggttctg gaatcgagag gctaaataat cgtaatccat
    41581 taacttcgaa tccatccatt gctcttaaaa aagtaagata atcaggttga ttcttaaaaa
    41641 gaatactaac ttcattacat aacttctcca ggcaattagt tgaattagaa ttaaattcta
    41701 tttggattat actttcaata aatgattcct tgagcggtgc ttcaaatgga tagtctaaat
    41761 cattcatcat ttttattaat ttatttattt gggttttagt catcattttg tgtggtccat
    41821 aat
//
LOCUS       DAJTNA010000012        40046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTNA010000012 DAJTNA010000000
VERSION     DAJTNA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40046
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.012"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(13..870)
                     /gene="murI"
                     /locus_tag="OO161_000555"
     CDS             complement(13..870)
                     /gene="murI"
                     /locus_tag="OO161_000555"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5461735.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(815..2663)
                     /gene="btuB"
                     /locus_tag="OO161_000556"
                     /pseudo
     CDS             complement(815..2663)
                     /gene="btuB"
                     /locus_tag="OO161_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2717..2907)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3021..4121
                     /gene="trmA"
                     /locus_tag="OO161_000557"
     CDS             3021..4121
                     /gene="trmA"
                     /locus_tag="OO161_000557"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5461736.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4161..4520)
                     /locus_tag="OO161_000558"
     CDS             complement(4161..4520)
                     /locus_tag="OO161_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5461737.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="OO161_000559"
     CDS             complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="OO161_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5461738.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5491..6891
                     /gene="sthA"
                     /locus_tag="OO161_000560"
     CDS             5491..6891
                     /gene="sthA"
                     /locus_tag="OO161_000560"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5461739.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="OO161_000561"
     CDS             complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="OO161_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5461740.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8056..9429)
                     /gene="argH"
                     /locus_tag="OO161_000562"
     CDS             complement(8056..9429)
                     /gene="argH"
                     /locus_tag="OO161_000562"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5461741.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDSLKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9490..10266)
                     /gene="argB"
                     /locus_tag="OO161_000563"
     CDS             complement(9490..10266)
                     /gene="argB"
                     /locus_tag="OO161_000563"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5461742.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLMLLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10274..11278)
                     /gene="argC"
                     /locus_tag="OO161_000564"
     CDS             complement(10274..11278)
                     /gene="argC"
                     /locus_tag="OO161_000564"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5461743.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11432..12583
                     /gene="argE"
                     /locus_tag="OO161_000565"
     CDS             11432..12583
                     /gene="argE"
                     /locus_tag="OO161_000565"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5461744.1"
                     /translation="MKNKLPPFIDIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLTSIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKESYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            12935..15586
                     /gene="ppc"
                     /locus_tag="OO161_000566"
     CDS             12935..15586
                     /gene="ppc"
                     /locus_tag="OO161_000566"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5461745.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTITGIAAGMRNTG"
     gene            15769..17502
                     /locus_tag="OO161_000567"
     CDS             15769..17502
                     /locus_tag="OO161_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5461746.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRHVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17717..18568
                     /locus_tag="OO161_000568"
     CDS             17717..18568
                     /locus_tag="OO161_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5461747.1"
                     /translation="MYHDISYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLI
                     EKPTTPE"
     gene            complement(18555..18896)
                     /locus_tag="OO161_000569"
     CDS             complement(18555..18896)
                     /locus_tag="OO161_000569"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5461748.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVDLLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18898..19776)
                     /locus_tag="OO161_000570"
     CDS             complement(18898..19776)
                     /locus_tag="OO161_000570"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5461749.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQCYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLISG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19742..22039)
                     /locus_tag="OO161_000571"
     CDS             complement(19742..22039)
                     /locus_tag="OO161_000571"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5461750.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22090..22410)
                     /locus_tag="OO161_000572"
     CDS             complement(22090..22410)
                     /locus_tag="OO161_000572"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5461751.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22425..23504)
                     /locus_tag="OO161_000573"
     CDS             complement(22425..23504)
                     /locus_tag="OO161_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5461752.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23813..26314
                     /gene="ptsP"
                     /locus_tag="OO161_000574"
     CDS             23813..26314
                     /gene="ptsP"
                     /locus_tag="OO161_000574"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5461753.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     IQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26326..26988
                     /gene="fsa"
                     /locus_tag="OO161_000575"
     CDS             26326..26988
                     /gene="fsa"
                     /locus_tag="OO161_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5461754.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            26999..28102
                     /gene="gldA"
                     /locus_tag="OO161_000576"
     CDS             26999..28102
                     /gene="gldA"
                     /locus_tag="OO161_000576"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR5461755.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWFVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28376..28993
                     /locus_tag="OO161_000577"
     CDS             28376..28993
                     /locus_tag="OO161_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR5461756.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29020..29925)
                     /gene="yijE"
                     /locus_tag="OO161_000578"
     CDS             complement(29020..29925)
                     /gene="yijE"
                     /locus_tag="OO161_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5461757.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGIHPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVALL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(30019..32198)
                     /gene="katG"
                     /locus_tag="OO161_000579"
                     /pseudo
     CDS             complement(30019..32198)
                     /gene="katG"
                     /locus_tag="OO161_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
     gene            complement(32527..33417)
                     /gene="metF"
                     /locus_tag="OO161_000580"
     CDS             complement(32527..33417)
                     /gene="metF"
                     /locus_tag="OO161_000580"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5461758.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33766..36198)
                     /gene="metL"
                     /locus_tag="OO161_000581"
     CDS             complement(33766..36198)
                     /gene="metL"
                     /locus_tag="OO161_000581"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5461759.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLIRAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDGFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36201..37361)
                     /gene="metB"
                     /locus_tag="OO161_000582"
     CDS             complement(36201..37361)
                     /gene="metB"
                     /locus_tag="OO161_000582"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5461760.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRIVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLSENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37638..37955
                     /gene="metJ"
                     /locus_tag="OO161_000583"
     CDS             37638..37955
                     /gene="metJ"
                     /locus_tag="OO161_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5461761.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38139..38747
                     /locus_tag="OO161_000584"
     CDS             38139..38747
                     /locus_tag="OO161_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5461762.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            38892..39567
                     /locus_tag="OO161_000585"
                     /pseudo
     CDS             38892..39567
                     /locus_tag="OO161_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39739..40011)
                     /locus_tag="OO161_000586"
     CDS             complement(39739..40011)
                     /locus_tag="OO161_000586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461763.1"
                     /translation="MELQLSDKGVIKMKAIQCCYFLALVSSNLMAGVMGNDNIFLSNS
                     DSAVLYCVAKIFPDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
BASE COUNT         9848 a        11041 c         9999 g         9158 t
ORIGIN      
        1 cattcaacca aatcagccta aaactgccag tttttcgagc gtttcgaagc cgtaacgctg
       61 taaaacgggc aataattgtt cagcttctgg cgtcattgcc atacaaaagg caatattcgc
      121 atcggcagat tttgcatccg gggcttcatg ttctaacaac caggccgttc ggcgagcaat
      181 cgctgcgcca gaatccacta gccgggttcc ctctggcagc acttgtaaca gttcttcttg
      241 tagtagaggg aaatgggtgc aacccaatac aacggtatct ggcggctctt tcattcttaa
      301 ccacgggcgt aggatacgtt ttagtgcatc cagagaaaca tcttcgccat gtagcttcgc
      361 ttcagccaac tcaaccatct ctgccgagcc cagcatttct atctggcatt cattagcgaa
      421 acgcgcgatc agctcatgag tataagaacg tttaactgtt ccgcgggttg ccagtaatcc
      481 gacaatgcca tttgccgtca gacgtgcagc aggtttaatc gccggcacga caccaacaac
      541 cgggaagtcg aacttttcgc gtaatgcagg aagtgaaacg gtactggcag tgttgcaagc
      601 gaccacagcc agcgcaaggg gataacgttc ttgcaccgcg gtgacaattg ccactactcg
      661 ctcaacaata aacgcttcgc ttttttcgcc atacgggaaa gcgacgttat cgaaagcata
      721 aatgtaatgg agatccggta agagatgccg gatctcgtca tagaccgaca acccaccgac
      781 gccggagtca aacaccagca cggtgggacg tggttcagaa ggtgtagctg ccagacaagg
      841 tgtattcccg tcctgcagtt tggtagccat agactgtctc ataatctttg tcgaacaggt
      901 tggctatttt accacgaact gtcaggtgag aggtgaccgg atacgcaacc gcaagatccc
      961 acaagctcac accgcccatt ttaacggttt gataaggata agatgagtaa tccttatcat
     1021 agcgagtgcc taaatactga taagtaatac cccagtcgaa gtcatacaac tgccagtcga
     1081 gctggtattt cacctgctgt ttagcacggc gtaacaacgg cgtgtcggta atcgcattgc
     1141 gcgcatcgac ataatcataa ctcacagtat gcgtcagtgg tccggtatca aaattggcgg
     1201 tcgcctcgac gcccttaatc cgcgctttcc cttcgttgta atatttcagg gtgtgatcat
     1261 cataatcgat caagtcactg acatcgttac gatatccgga aatacgccag ttcaccccag
     1321 cggttaagcc ttcaaacgcg ccttcccact gtttgctttt ctccgggtcc agattcggat
     1381 ttccgtagaa gccatacagt tgccccagat ttggtgcctt ataagatgtc ccgtaggaag
     1441 caatgaagcg ataaccttcg atgaattccc aaccggcgct ggtttgccag gttccatgac
     1501 gaccaaactg tgagttatcg tcactgcgtg ctgcgccttc aaaggtaaaa tcgccgactt
     1561 gttgcagccc ggtcagatag atgccggtat tacgttgatc atatccatcc tcaacataac
     1621 ctgtacccgg cgtcgtagtc tgtttctgcc agtcgacacc cgcaccaata ctaccgtgac
     1681 caacgatgac attgtttgcc cactggacgg tgtattgctt catctcatcg agcgtcgccg
     1741 acgaatcata acgaccataa tggggatcgt agttgtaatc tttgctatgg ctatagctgg
     1801 taatgagttg tgatttaatc agttcgccgt tatagcgcag cccggcgtcc caactttggc
     1861 tatagagttt acgggtatcg agcaacgatg aaccgggaga ataatacgcg tcataattgg
     1921 tacggttatc atagccatag ccgcgcacaa agccgctcca ggcatcagta aagttatgct
     1981 ccagcgcgcc ataaagcgtt ttacttaaaa aaccatcgtt atctgtctgc gcttgcgttc
     2041 cggtattacc ataggcaaca acatcataac catgagtatg ggcataatcg cccaacagcg
     2101 ttacccgtgt cttatccccc agttgttgct gcgtagagac atcatagttc tgataactat
     2161 tgcttcccca ccctgctgaa atttccgttc cgggttcatc gcgcgtcgtg atgatattca
     2221 ccaccccgcc tattgcatcg gaaccataaa cagcggagcg cggcccacgg atatattcaa
     2281 cacgctggac aagcgcacgc aatagggaac tggctaaggt cggcagaacc actcaccccc
     2341 gccagattca gccgtacgcc atcaattaac accaacacat gactggcatt tgtaccgcga
     2401 ataaaaatag atgagagctg acctgaaccg ccgttttggg tgatatcgac gcccggaaga
     2461 cggcgcagca catcattgac cgaggtcgac tgccagcggt cgatatcctg acgggtcaca
     2521 acggtggttg gtgcaagcac agtgctgcgc ggctgttcaa aacggttagc agtaacgacg
     2581 agagtatccg ggctggtatc ctgtgcccaa gcggaaaagg ctgtgacaga acacgccgtc
     2641 agcagcgaag cttttttaat cattgtaaag catccacaat agaagaagga tgccgcaggt
     2701 ttcatcaata ttacgcgatg atgagacgtt ggccggcagg tcttcgggct tggaggggta
     2761 tctaagatac taagagatga tgacttccca ccggatggca gtgtccgcat aacgcaatca
     2821 tcgcaccttt ccttaccgct gcgcgtcagc tccagattcg cactggattc cctattaact
     2881 cacaggaccg gcaagtggat gctacaggtt gtaacaagtt actgtccaga cgtagctcac
     2941 aaataggaat tcatcaagat ctggacatct gatgagcaat ccctacaatc gccgcgtact
     3001 ttaatttttc aggatacatc atgacccccg aacaccttcc aacagaacag tatgaagcgc
     3061 agttagccga aaaagtggta cgtttgcaaa gtatgatggc accgttttct gacctggttc
     3121 cggaagtgtt tcgctcgccg gtcagtcatt accggatgcg tgcggagttc cgcatctggc
     3181 acgatggcga tgacctgtat cacatcattt tcgatcaaca aaccaaaagc cgtatccgcg
     3241 tggatagctt ccccgccgcc agtgaactta tcaaccagtt gatgacggcg atgattgcgg
     3301 gtgtgcgtaa taatcccgtt ctgcgccaca agttgttcca gattgattac ctcaccacgc
     3361 tgagtaatca ggcggtggtt tccctgctat accataagaa gctggatgat gagtggcgtc
     3421 aggaagcgga ggccctgcgc gatgcactgc gcgcgcagaa tctgaatgtg catctgattg
     3481 gtcgggcaac gaaaaccaaa atcgagctgg atcaagatta catcgacgag cgtctgccgg
     3541 tcgcagggaa agagatgatc taccgtcagg tagaaaacag ctttacccag ccgaacgcgg
     3601 cgatgaatat tcagatgctg gaatgggcgc tggacgtaac caaaggctca aaaggcgatt
     3661 tactggagct gtactgcggc aacggtaact tttcattagc gctggcgcgc aattttgatc
     3721 gggtattagc caccgaaatc gctaagccgt cggttgctgc tgcgcaatac aacatcgcag
     3781 ctaaccatat tgataacgta caaattattc gtatggcggc agaagaattt actcaggcga
     3841 tgaatggtgt gcgcgagttt aaccgcctgc aagggatcga cttaaagagt tatcagtgcg
     3901 aaaccatttt tgtcgaccca ccgcgcagcg gtctggacag tgaaaccgag aaaatggtgc
     3961 aggcgtatcc gtgtattttg tacatctcct gtaacccgga aacgttatgc aagaatctgg
     4021 aaacattaag ccagacgcac aaggtcgaac gtctggctct gtttgatcag ttcccctaca
     4081 cgcaccatat ggagtgcggc gtattactga ccgcgaagta aaacccatgc cggatgcgcc
     4141 agcatccggc ataataccga ttactcagca acttcctgct tacggttacg catcttcgcg
     4201 ccaatccaga acaccataat gacggagagt actgccggga agaagttaga gccgatatcc
     4261 ggatattccg cacgaactac cgtgctgtac agcagtacgc cgagaataaa acaggcagct
     4321 gccagacccg gcaagcctac cggcatagtg cgattaagat aacgttgatg caggcagtaa
     4381 accgtcagca ccagggaaat aatcgggaat acagaaaatg gcacaatgga gctaaacagc
     4441 gctgcgaaag taccattaat cgataagcca gcaactaacg ccagcagcag cgtacctcta
     4501 tcttgatttg cttgtttcat tactcgtcct tcacatttcc cggaataatt gcggttttct
     4561 cttgttcacg gcgataccag taataagccc ctttcgaaat cattcgcagt tgcagtacca
     4621 gtcgctcttc taattgccga cgttgttcga cgccgacgtc caacgcctcg gcacccgcac
     4681 tgaagacaat tgtcaccatt gcttcggctt gcgcttcagt aaacgcacgc ggcatatggt
     4741 tttcgagttc cagatagtcc gcaagttccg caatgaagtg ctgaatttca cgcgcaacgg
     4801 cggcacgaaa cgcagcggag gtgccggagc gttcccgcaa taataaccgg aaggcgttag
     4861 gattattacc gatgaactcc ataaatgtgg agaccgaggt gcggatcaca ctcccgcctt
     4921 tggcgatacg ctgacgcgcc tggcgcatga gttggcgtag cattaaaccg ctctcatcaa
     4981 ccatggtcag acccagttcg tctacgtcgc ggaaatgccg ataaaaagag gtgggagcaa
     5041 tgcccgcttc acgcgccact tcacgcaaac tcaggctggc gaagctgcgt tcagcactta
     5101 attggctaaa tgcggcttcc accagcgaac ggcgggtttt ttctttttgt tgcgctctta
     5161 tgcccatcac gatgtctgaa tccttgccaa aagtactgct ggcactatac cagagaatga
     5221 acatactgga ttgctccagt attgtgaatg aacggtaacg caataaaaca tatttactgg
     5281 ccggttcttt tacgtacagc ggaaacctgc cgcttaaacg gagagtatcg tcgataaaaa
     5341 tccaataaaa cgtcagggca aaagtaagaa acagacaaag caaaggccgc tcaggatata
     5401 gccagataaa tgacggggat caattggctt acccgcgata aaatgttacc attctgttgc
     5461 ttttatgtat aagaacaggt aagccctacc atgccacatt cctacgatta cgatgccata
     5521 gtaataggtt ccggccccgg cggcgaaggc gctgcaatgg gcctggttaa gcaaggtgcg
     5581 cgcgtcgcag ttatcgagcg ttatcaaaat gttggcggcg gttgcaccca ctggggcacc
     5641 atcccgtcga aagctctccg tcacgccgtc agccgcatta tagaattcaa tcaaaaccca
     5701 ctatacagcg accattcccg actgctccgc tcttcttttg ccgatatcct taaccatgcc
     5761 gataacgtga ttaatcaaca aacgcgcatg cgtcagggat tttacgaacg taatcactgt
     5821 gaaatattgc aggggaacgc tcgctttgtt gacgagcata cgttggtgct ggattgcccg
     5881 gacggcagcg tcgaaacact aaccgctgaa aaatttgtta ttgcctgcgg ctctcgtcca
     5941 tatcatccaa cagatgttga tttcacccat ccacgcattt acgacagcga ctcaattctt
     6001 agcatgcacc acgaaccgcg ccatgtactt atctatggtg ctggagtgat cggctgtgaa
     6061 tatgcgtcga tcttccgcgg tatggatgta aaagtggatc tgatcaacac ccgcgatcgc
     6121 ctgctggcat ttctcgatca agagatgtca gattctctct cctatcactt ctggaacagt
     6181 ggcgtagtga ttcgtcacaa cgaagagtac gagaagatcg aaggctgtga cgatggtgtg
     6241 atcatgcatt tgaagtcggg taaaaaactg aaagctgact gcctgctcta tgccaacggt
     6301 cgcaccggta ataccgattc gctggcgtta cagaacattg ggctggaaac tgacagccgc
     6361 ggacagctga aggtcaacag catgtatcag accgcacagc cgcacgttta cgcggtgggc
     6421 gacgtgattg gttatccgag cctggcatca gcggcctatg accaggggcg cattgccgcg
     6481 caggcgctgg taaaaggcga agccaccgca catctgattg aagatatccc taccggcatt
     6541 tacaccatcc cggaaatcag ctctgtgggc aaaaccgaac agcagctgac cgcaatgaaa
     6601 gtgccatatg aagtgggccg cgcccagttt aaacatctgg cacgcgcaca aatcgtcggc
     6661 atgaacgtgg gcacgctgaa aattttgttc catcgggaaa caaaagagat tctgggtatt
     6721 cactgctttg gcgagcgcgc tgccgaaatt attcatatcg gtcaggcgat tatggaacag
     6781 aaaggtggcg gcaacactat tgagtacttc gtcaacacca cctttaacta cccgacgatg
     6841 gcagaagcct atcgggtagc tgcgctaaat ggcttaaacc gcctgtttta acactttatc
     6901 gaaatggcca tccattcttg cgcggatggc ctctgccagc tgctcatagc ggctgcgcag
     6961 cggtgagcca ggacgataaa ccaggccaat cgtgcggcga ggttccggct taatacacgg
     7021 caaataaaca accccatcgc gtttgcgctc cggcggcaca gccagcgcag gcagtaaagt
     7081 gatcccgcta cctgccgcca ccatgttacg cagtgtctcc aggctggtcg cgcggaagtg
     7141 tgtatcttca tccgccccgg cttcaaagca gaaacccatt gcctgatcgc gcaaacagtg
     7201 accatcttcc agcatcagca gtttttcccc tgccagatcg gccatcggta cgcattcgcg
     7261 gttcgcccac gggtgatctt catagatagc cagcaacatt ggctcatcaa acaacggcac
     7321 ttcaatgaat gcttcgctct ctttcaccag cgcgaggatc acgcaatcga gtttgccgct
     7381 gtccagttgc gccagtaact ggtgggtctg tgcttcatgc agatacattt ccagctttgg
     7441 aaatgtctgg tgcagcatcg ggataatatg cggcagcagg tacggtccaa ccgtaggaat
     7501 caaaccaata tgcagcggtc cggacatcgt ctcgccctgc tggcttgcca tctctttaag
     7561 gactttcacc tcacgcagca cggtacgcgc ctgatccacc agcagcattc ccgcctgggt
     7621 gaacaacact ttacggctgg tccgctccag caacatcacg cccagctcat cttccagctt
     7681 acgaatttgg ccgctaagcg tcggctggct aacgtggcag gaatctgccg cacgccgaaa
     7741 atggcggtgt tcagccaatg ccaccaggta ctcaagatca cgaatattca ttatccatcc
     7801 tccatcgcca cgatagttca tggcgatagg tagaatagca atgaacgatt atccctatca
     7861 agcattctga ctgataattg ctcacagaaa cggagcggca cctcttttaa cccttgaagt
     7921 cactgcccgt ttcgagagtt tctcaactcg aataactaaa gccaacgtga acttttgcgg
     7981 atctccagga tccgcttttt tgccataaaa aagcccggcg ataagccagg ctcaaatttg
     8041 tacatataaa tgttcttacc ccaaccgcgc ctgcgcaaaa gcaatcgctt gggccacctg
     8101 ctgcggtgag acgccgcctt ttgccgcacg cttgtcgagg cacgattgta gcgacagaat
     8161 cggatagaca tcttcgccaa tcacgtggct gaatttctgc aactcgtcga gcggcagatc
     8221 ttccagcggt ttgccctgac gaatggcttc caccaccgct tcacccacga tatggtgcgc
     8281 ctcgcggaaa ggtacgcctt tcgccaccag gtaatccgcc agttcggtgg cgttggcgta
     8341 accctgctga gccgcttcct ggcaacgtgg acgtttcacc tgaatgccgt ccagcaccag
     8401 cgccgccata tgcaggcagt ccagccaggt atcgagcgcg tcgaacagac cttctttgtc
     8461 ttcctgcata tctttgttgt aagccagcgg caaacctttc agcgtcatca tcatgccggt
     8521 taacgcaccc tgcacccgac cgcatttacc gcgaatcagc tccagcgcat ccgggttttt
     8581 cttctgcggc attaatgatg aaccggaagt cacgcggtca gaaagctcca caaaccccgc
     8641 ttcgccggtg ttaaagaaaa tcagatcttc agcaaaacgc gacagatgca ccatgccgat
     8701 agcggcagcc gacagcagtt ccaacacatg gtcacggtca gaaacgctgt ccagactgtt
     8761 acgggtcgcc gaagcaaagc ccagccagcc tgctaactgt tcacggtcga tttcataggc
     8821 cgttcccgcc agcgcgccac agcctaatgg gctgacatcc agacgcttaa gcgaatcctg
     8881 caaacggctt tcatcacgcg ccagcatctc aacataggcc aggcaccagt gcgcgaacgt
     8941 caccggctgg gcgcgttgca ggtgagtgta acctggcatt accgcgtcct gattgttttg
     9001 tgcggtttcc accagcgccg attgcagctg ccggttagcc gtcagtaact cgctaacggt
     9061 atctttgcac cacagtttca ggtcagtcgc tacctgatca ttacggctac gcccggtatg
     9121 cagctttttg cctaactggc ccactttgtc gatcagtttg ccttccaccc agctatggat
     9181 atcttcggcg tcgctttcaa ggatttgttg tggcctagcg cgaacatctt ccagcaacac
     9241 gttcagcgcc tcttccagtt gcgcctgctc ttctgcggtt aacacgccga ccgttaccag
     9301 ggctttggac caggccacag agccaacaat atcctgctcc gccagacggt aatcaaagcg
     9361 cagtgagtcg ttgaattgtt tgaaccgttg atctgctgcc tgggtaaaac gcccgcccca
     9421 aagtgccata actctgtttc cttattttga aattcaatgc cggaaagctg cgcctccggc
     9481 taacaaaact taagctaaaa tccgcgtacc catcggcata ccgttaaaca gtgccggaag
     9541 ctgctccgca tgacgccagg aggcgatatc taccggacgg cccagcgtgc gggccgcatc
     9601 cagcgccgcg ttcactttca ctatcatgcc gtcagtaata atgccctgct caatcagttg
     9661 ttctgctttc gcggcggtca tttcggcaat gcgttgccct ttgccgtcga gaatgccgct
     9721 gacgtcggag agcaacatca gatccgcgcc cagcgttgcc gccagcgccg ttgccgcctg
     9781 gtcggcattg acgttcatca gttgcccttc gtctgttacg ccaatggagc tgaccaccgg
     9841 cagataaccg ttctccagca aggagttgat aagcttaggc gaacctggct gcgccagtcc
     9901 aacatgacct aactcttcat caagctgggt cactttgacg ctgtcgccat caccgagaaa
     9961 caaacctacg gccgcaatct gatgtttctt cgcccatgcc aacagggttt tatttgccgt
    10021 tcccgccagt gctccggtga taatgtctat ctgatcagca ggcgtcaccc gcaggccgtt
    10081 tttctttttc accggcagat tcagcccttt catcagctca tccaccacgc aaccgccgcc
    10141 gtgcacaatc accagcggac gctgatgtga ctcacgataa ttcaccagtg cgctaaacag
    10201 acgttccagc gcctcttcac tatccagcag tacgccgccc agtttgataa ttaatggatt
    10261 catcattgca cccttaaata agagactgcg tttccgcata gccgaaacga atattggcgc
    10321 actgtaccgc ttgtgccgcc gcgcctttca gtaagttgtc ttcggtcgcc acaataatca
    10381 gatgctcgcc ctgaacggca aacccgatat cgcaaaatgg cagcccaacg acatttttca
    10441 gcgccggaac gcctttgtca tacagccgca ccagcggttt atgggcatac gcctgctgta
    10501 acacttgcgc gacttgcgcc tgggtcacac ccgatttcag gcggcaggta atggtttcga
    10561 gaatgccgcg cgggaaattg cccagatgtg gggtgaagat aacgtcagca ccgaggtgtg
    10621 tggcgatctc tggttgatgg cgatgagtaa agacgccata cggttgcagg ctaacttcac
    10681 aaaaactatt tgaaatcgct gccttacgtc ctgcaccgct cacgccgctg gtggcgttga
    10741 tcgccggcca ctgattgagg tcaagaagat cggcatcaat caacggtttc agcgccagct
    10801 gtgccgccgt cggataacag cctggcaccg caatcaaatt cgcttctttt aatttattac
    10861 cgcaccactc cgccagaccg taggctgcct gttccagcag ttccgggtat tgatgggtaa
    10921 agccgtaata tttttcatag aaggtggcgt cgttaacacg aaacgcgccg gaaaggtcga
    10981 acaccacgca gcccgcttca agaaactgcg gcgctaaatc gtggctaact tcatgggcgg
    11041 tggcgagaaa cactacgtcc acccctgggc taaactcgct gatatccgac atcggctgca
    11101 acggcagctc aacgatgcct tttagctgcg gatgcaaatc ggagattaac tttcccgcat
    11161 cattgctttg cgctgaaaca gtcaaagcgg ttatgttcat atgcggatgg cgatttacat
    11221 aggtcactag ctctgcgcca gcgtagccgc tggcacccac aatcagcgta ttcaacatcg
    11281 gggctattca ccttcttatg tctggttgcc aggttaaacg taaaacattc accttacggc
    11341 tggtgggttt tattacgctc aacgttagtg tatttttatt cataaatact gcatgaatat
    11401 tgatactatc atgaccagag gtgtgtcaac aatgaaaaac aaattaccgc catttatcga
    11461 tatttaccgc gctctgattg ccacaccttc aataagcgct acggaagagg cactcgatca
    11521 aagcaatgca gatttaatca ctctgctggc ggactggttt aaagatttgg gcttcaatgt
    11581 ggaagtgcag cctgttccag gaactcgcaa caaattcaat atgctgacaa gtatcggaca
    11641 gggggctggc ggcttgttgc tggcggggca taccgatacg gtgccatttg atgacggtcg
    11701 ctggacgcgc gatccgttta cactgacgga gcatgacggc aagctttacg gcttaggcac
    11761 cgccgacatg aaaggctttt ttgcgtttat ccttgatgcg ctacgcgatg tcgacgtcac
    11821 gaaactgaaa aaaccgctct acattctggc gactgctgat gaagaaacca gtatggccgg
    11881 agcgcgttat tttgccgaaa ctaccgccct gcgcccggat tgcgccatca ttggcgaacc
    11941 gacgtcacta caaccggtac gcgcacataa aggtcatatc tctaacgcca tccgtattca
    12001 gggccagtcg gggcactcca gcgatccagc acgcggagtt aacgctatcg aactaatgca
    12061 cgacgccatc gggcatattt tgcaattgcg cgataacctg aaagaaagtt atcactacga
    12121 agcatttacc gtgccatacc ctacgctcaa cctcgggcat attcacggtg gcgacgcttc
    12181 taaccgtatt tgcgcttgct gtgagttgca tatggatatt cgtccgctgc ctggcatgac
    12241 actcaatgaa cttaatggtt tgctcaacga tgcattggct ccggtgagcg aacgctggcc
    12301 gggtcgtctg acggtcgacg agctgcatcc gccgatccct ggctatgaat gcccaccgaa
    12361 tcatcaactg gttgaagtgg ttgagaaatt gctcggagca aaaaccgaag tggtgaatta
    12421 ctgtaccgaa gcgccgttta ttcaaacgtt atgcccgacg ctggtgctcg gtccgggttc
    12481 catcaatcaa gctcatcagc ctgatgaata tctggagacg cggtttatta aacccacccg
    12541 tgagttgatt atccaggtaa ttcaccattt ttgctggcat taaaacgtag gccggataag
    12601 gcgctcgcgc cgcatccggc accgttgcca aactccggtg ccgcaataat gtcggatgcg
    12661 atgcttgcgc atcttatccg acctacacct ttggtgttat gtatggcgat tttttaacat
    12721 ttccataagt tacgcttatt taaagcgtcg tgaatttaat ggcgtaaatt cctgctattt
    12781 attcgtttgc tgaagcgatt tcgcagcatt tgacgtcacc gcttttacgt ggctttataa
    12841 aagacgacga aaagcaaagc ccgagcatat tcgcgccaat gcgacgtgaa ggatacaggg
    12901 ctatcaaacg atcagatggg gtgtctgggg taatatgaac gaacaatatt ccgcattgcg
    12961 tagtaatgtc agtatgctcg gcaaagtgct gggagaaacc atcaaggatg cgttgggaga
    13021 acacattctt gaacgcgtag aaactatccg taagttgtcc aaatcttcac gcgctggcaa
    13081 tgatgctaac cgccaggagt tactcaccac cttacaaaat ttgtcgaacg acgagctgct
    13141 gcccgttgcg cgtgcgttta gtcagttcct gaacctggcc aacaccgccg agcaatacca
    13201 cagcatttcg ccgaaaggcg aagctgccag caacccggaa gtgatcgccc gcaccctgcg
    13261 taaactgaaa aaccagccgg aactgagcga agacaccatc aaaaaagcag tggaatcgct
    13321 gtcgctggaa ctggtcctca cggctcaccc taccgaaatt acccgtcgta cactgatcca
    13381 caaaatggtg gaagtgaacg cctgtttaaa acagctcgat aacaaagata tcgctgacta
    13441 cgaacacaac cagctgatgc gtcgcctgcg ccagttaatc gcccagtcat ggcataccga
    13501 tgaaatccgt aagctgcgtc caagcccggt agatgaagcc aaatggggct ttgccgtagt
    13561 ggaaaacagc ctgtggcaag gcgtaccaaa ttacctgcgc gaactgaacg aacaactgga
    13621 agagaacctc ggctacaaac tgcccgtcga atttgttccg gtccgtttta cttcgtggat
    13681 gggcggcgac cgcgacggca acccgaacgt cactgccgat atcacccgcc acgtcctgct
    13741 actcagccgc tggaaagcca ccgatttgtt cctgaaagat attcaggtgc tggtttctga
    13801 actgtcgatg gttgaagcga cccctgaact gctggcgctg gttggcgaag aaggtgccgc
    13861 agaaccgtat cgctatctga tgaaaaacct gcgttctcgc ctgatggcga cacaggcatg
    13921 gctggaagcg cgcctgaaag gcgaagaact gccaaaacca gaaggcctgc tgacacaaaa
    13981 cgaagaactg tgggaaccgc tctacgcttg ctaccagtca cttcaggcgt gtggcatggg
    14041 tattatcgcc aacggcgatc tgctcgacac cctgcgccgc gtgaaatgtt tcggcgtacc
    14101 gctggtccgt attgatatcc gtcaggagag cacccgccat accgaagcgc tgggcgagct
    14161 gacccgctac ctcggtatcg gcgactacga aagctggtca gaggccgaca aacaggcgtt
    14221 cctgatccgc gaactgaact ccaaacgtcc gcttctgccg cgcaactggc aaccaagcgc
    14281 cgaaacgcgc gaagtgctcg atacctgcca ggtgattgcc gaagcgccgc aaggctccat
    14341 tgccgcctac gtgatctcga tggcgaaaac gccgtccgac gtactggctg tccacctgct
    14401 gctgaaagaa gcgggtatcg ggtttgcgat gccggttgct ccgctgtttg aaaccctcga
    14461 tgacctgaac aacgccaacg atgtcatgac ccagctgctc aatatcgact ggtatcgcgg
    14521 cctgattcag ggcaaacaga tggtgatgat tggctattcc gactcagcaa aagatgcggg
    14581 cgtgatggca gcgtcctggg cgcaatatca ggcacaggat gcattaatca aaacctgcga
    14641 aaaagcgggt attgagctga cgttgttcca cggtcgcggc ggttccattg gtcgcggcgg
    14701 cgcacctgcc catgcggcac tgctgtcaca accgccagga agcctgaaag gcggcctgcg
    14761 cgtaaccgaa cagggcgaga tgatccgctt taaatatggt ctgccagaaa tcaccgtcag
    14821 cagcctgtcg ctttataccg gggcgattct ggaagccaac ctgctgccac cgccggagcc
    14881 gaaagagagc tggcgtcgca ttatggatga actgtcagtc atctcctgcg atgtctaccg
    14941 cggctacgta cgtgaaaaca aagattttgt gccttacttc cgctccgcta cgccggaaca
    15001 agaactgggc aaactgccgt tgggttcacg tccggcgaaa cgtcgcccaa ccggcggcgt
    15061 cgagtcacta cgcgccattc cgtggatctt cgcctggacg caaaaccgtc tgatgctccc
    15121 cgcctggctg ggtgcaggta cggcgctgca aaaagtggtc gaagacggca aacagagcga
    15181 gctggaggct atgtgccgcg attggccatt cttctcgacg cgtctcggca tgctggagat
    15241 ggtcttcgcc aaagcagacc tgtggctggc ggaatactat gaccaacgcc tggtagacaa
    15301 agcactgtgg ccgttaggta aagagttacg caatctgcaa gaagaagaca tcaaagtggt
    15361 gctggcgatt gccaacgatt cccatctgat ggccgatctg ccgtggattg cagagtctat
    15421 tcagctacgg aatatttaca ccgacccgct gaacgtattg caggccgagt tgctgcaccg
    15481 ctcccgccag gcagaaaaag aaggccagga accggatcct cgcgtcgaac aagcgttaat
    15541 ggtcactatt accgggattg cggcaggtat gcgtaatacc ggctaatctt cctcttctgc
    15601 aaaccctcgt gcttttgcgc gagggttttc tgaaatactt ctgttctaac accctcgttt
    15661 tcaatatatt tctgtctgca ttttattcaa attctgaata taccttcaga tatccttaag
    15721 gaattgtcgt tacattcggc gatatttttt tcaagacagg ttcttactat gcattccaca
    15781 gaagtccagg ctaaacccct ttttagctgg aaagccctgg gttgggcact gctctacttt
    15841 tggtttttct ctactctgct acaggccatt atttacatca gtggttatag tggcaccaac
    15901 ggcattcgcg actcgctgtt attcagctcg ctgtggttga tcccggtatt cctctttccg
    15961 aagcggatta aaattattgc cgcagtaatc ggcgtggtgc tatgggcggc ctctctggcg
    16021 gcgctatgct actacgtcat ctacgggcag gagttctcgc agagcgttct gtttgtgatg
    16081 ttcgaaacca acaccaacga agccagcgag tatttaagcc agtatttcag cctgaaaatt
    16141 gtgcttatcg cgctggccta tacggcggtg gcagttctgc tgtggacacg cctgcgcccg
    16201 gtctatattc caaagccgtg gcgtcatgtt gtctcttttg ccctgcttta tggcttgatt
    16261 ctgcatccga tcgccatgaa tacgtttatc aaaaacaagc cgtttgagaa aacgttggat
    16321 aacctggcct cgcgtatgga gcctgccgca ccgtggcaat tcctgaccgg ctattatcag
    16381 tatcgtcagc aactaaactc gctaacaaag ttactgaatg aaaataatgc cttgccgcca
    16441 ctggctaatt tcaaagatga atcgggtaac gaaccgcgca ctttagtgct ggtgattggc
    16501 gagtcgaccc agcgcggacg catgagtctg tacggttatc cgcgtgaaac cacgccggag
    16561 ctggatgcgc tgcataaaac cgatccgaat ctgaccgtgt ttaataacgt agttacgtct
    16621 cgtccgtaca ccattgaaat cctgcaacag gcgctgacct ttgccaatga aaagaacccg
    16681 gatctgtatc tgacgcagcc gtcgctgatg aacatgatga aacaggcggg ttataaaacc
    16741 ttctggatca ccaaccagca gacgatgacc gcccgcaata ccatgctgac ggtattttcg
    16801 cgccagaccg acaagcagta ctacatgaac cagcaacgta cgcagagtgc gcgtgaatac
    16861 gacaccaacg tgctgaagcc gttccaggat gtgctgaatg accctgcgcc gaagaaactg
    16921 atcatcgttc atctgctggg tacgcatatc aaatacaaat accgctaccc ggaaaatcag
    16981 ggcaagtttg atggcaatac cgatcatgtt ccgccaggat taagcgcaga agagctggaa
    17041 tcatataacg attatgacaa cgctaacttg tataacgatc atgtggttgc cagcctgatt
    17101 aaagacttta aagcggcaga cccgaacgga ttcctggttt acttctctga ccacggtgaa
    17161 gaggtttacg acacgccgcc gcacaaaact caggggcgta acgaagacaa cccgacgcgc
    17221 cacatgtaca ccattccgtt cctgctgtgg acgtcggaaa aatggcaagc gactcatccc
    17281 cgtgatttct cacaggatgt cgatcgtaaa tacagcctgg cggaactgat ccacacctgg
    17341 tcagatttgg cgggcttatc ttacgacggt tacgatccaa cccgttcagt ggtgaatccg
    17401 cagttcaaag aaactacccg ctggattggt aacccgtaca agaaaaacgc gctgatcgat
    17461 tacgacactc tgccgtatgg cgaccaggta ggtaatcagt aattatcagc caggcgctat
    17521 ttgccggatg cgacgcttga cgcgtcttat ccggcctaca gatcgcggcg gtaggctgga
    17581 taaggcgttt acgccacatc cggcaataca actgccgcaa tgtttacatc gaactcccct
    17641 caggccacac ttttcaggtg gcctttttta ataatgccag ttactgtttt cccgtctaca
    17701 tacctaaggg aataacatgt atcacgacat cagctatctg ctctcccgcc tgatcaacgg
    17761 cccgctgtcc ctgcgccaga tttactttgc cagcagtaac ggcccggtgc ccgatcttgc
    17821 gtatcaggtc gattttccac ggctggaaat tgtgctggaa ggtgagtttg ttgataccgg
    17881 cgctggagca acgttagttc ccggcgatgt gctgtacgtt gccgctggtg gctggaattt
    17941 cccacaatgg aaaacacccg ctaccacctt tagcgtgctg tttggcaaac agcaactcgg
    18001 cttcagcgtc gtgcaatggg atggcaaaca atatcaaaat ctggcgaagc aacacgtcgc
    18061 ccgacgcggc ccacgtatag gttcttttct gctacaaacg ctcaatgaaa tgcaaatgca
    18121 gccgcaggag cagcaaacgg caaggcttat cgtagccagc ctgcttagcc actgccgcga
    18181 tttgctcggc agccaaatcc agaccgcctc acgcagccag gcactatttg aagctattcg
    18241 cgattatatc gacgaacgct atgcctccgc gcttacccgc gaatctgtcg cacaggcatt
    18301 ctatatttca ccaaactatc tctcgcacct gtttcaaaaa acgggggcca ttggttttaa
    18361 cgaatacctg aatcacacgc gactggagca cgctaagacg ttattgaaag gctatgacct
    18421 gaaggtaaaa gaagtggcgc acgcctgcgg ctttgtcgac agcaactact tctgccgtct
    18481 gtttcgtaaa aacaccgaac gctcgccgtc ggaatatcgc cggcagtacc acagccaact
    18541 gatagaaaaa ccgactactc cagaataaga tgggtttgct gcggcgcaga aagcactttg
    18601 cgcaccgcgc tcattacccg ctgcggctca cgcaggaatg cgtagatgct gcattgcaca
    18661 tagcggcaat gttcaaatcg ctcggcaccg gcaagctcga tatccgtaat caacagatca
    18721 acatccgccc gacggatatc ctcgtccgct aaacgattct ccgttcccag cgcgccctga
    18781 gtttcaatgc tgacgcccca cttctctaac tggcacaact tttccagccg ttccgccgcc
    18841 atataagtat gcgccacgcc gcttacgcag gcggttactg ccaccaggta tgccatttta
    18901 acctcccacg gtaacctgaa atccggcccg ttctgccatt tcgcgcatcg ttgccacatc
    18961 ggctgatgac ggcgcagcaa catccttcat cgaccatgtt ttccccagca ggcggtattt
    19021 cggctcgccg tactgatgaa acggcaacag atggatctgc ctgatattca gcgggatcag
    19081 cacgtccagc gcctgctgca tattctcccg gctgagtgtg aaaccggaga tcagcggcaa
    19141 acgcgggatc acgttgacac cctcactcac cagcaaacgc aggttctcca gtacgcgtgg
    19201 caggttcatc ttcaccacat cccgcgcctg agtcgcgtcc ataattttta aatcgaacaa
    19261 cacttcatcg cacaatttcg ccagcggtaa cagcttgctg gctggtgcgt ctccggcagt
    19321 ttcaatggca catgagacac cccacagccg cagtcgctgt aaaaaacggg tagcaaactc
    19381 cgcctgcatt aacacttcgc cgccagaaag cgtgacgccg ccgccggacg tgcgaaaaaa
    19441 aatgtcatct ttcatcactt cccgttccag agcgtcaagg ctgatatcgc gaccaatccg
    19501 ttcaaacgcc ccggaggggc attcatccgc atcacgcaaa catttcgcac agtgcagaca
    19561 tttcgcctct ctgcgtaccg tctggatttt gccggagatc gactccggat tagcacacca
    19621 cgggcaaaga tgcggacagc ctttaaaaaa gaccaccgta cgaatgccct cgccgtcatt
    19681 cagtgaataa cactgaatgt tgaaaatgcg cgccacatca ttgcggcgcg tttccacaac
    19741 gttacagctg atgcgctgtc cggcggatga tgtcatcctg gatctccttc gacagttcga
    19801 caaagaaggc gctgtatccg gcaacgcgca ccaccagccc ggcataatct tgtgggcgct
    19861 gttgcgcttc ccacaacgtg tcggcgttca ccacgttaaa ctgaatatgc tgcaacttaa
    19921 gctgggtaaa cgcccgtaag aagtcggcca gtttgcgtaa tcccgcttca ccttccaggg
    19981 tcgccggagt gaatttcacg ttcagcaacg taccgttaga cagcagcgta ttatcgagct
    20041 tactgactga cttcagtacc gctgttggcc cttgttcgtc ctggcccagc ataggtgaca
    20101 agccgccgtc tgccagctgt tctccggcaa aacgaccgtc tggcgtcgcg ccaaccaccg
    20161 accccaacgg gacgtgagca gaaacggtat acgatcccgg cgtgaagtaa ccaccgcgcg
    20221 ggttctggta tttttccact tctttgcagt agtggcgcaa cagttcggcg ctaatgttat
    20281 ccacctcgtc gatatcgtta ccatatttct caaagcggtt aattaagcga gcgcggactt
    20341 tttcgccttc tggcgttgca aagttggctt ttaataccga cagcaattcg tcaaaactta
    20401 aacgctgttg ctcaaaaacc atccctttca acgcatggag agaatcgctc aggttggcga
    20461 taccgatccc ctgtacgccg gagaagttat aacgcgcgcc gccatcggta atatcgcgac
    20521 ctttttccag acaatcgctg ataaaagacg agagcagcgg tacaggtgcc cagtcgcgat
    20581 ggccgatatc acaaatattg ctgccctcaa ccatcagggt gatgtagtgg ctgatcttgg
    20641 cgcggatctg ctccagtaaa ccttcataag tcagcgcagc attgccttca ttttcatgca
    20701 ggcagatttc catcactttc agcagattaa acatcgcgat gtcatgcaag ccgtaggttc
    20761 tgccgggaat agataattcc acacagccca ctacggaata gtcgcgcgca tcttccagcg
    20821 aaacgccacg gttgaggaac gctggtacca ccacttcatc gttaaagatt tgcggaatac
    20881 cggtgccgag gcgaatggtt tcggcggttt tcatcaggaa tggcgtgtcg atcaaggcgt
    20941 tagtgcgcac gccgaggttc ggttgcggta attgcacgct ttgataggcg tcaaggcaaa
    21001 ggaacgaaag cacgttcacc gcgctacgtc cgttctcggt taacccgccg agcagtgcgg
    21061 tatagccggt cgggaaacct gcgaaataac gcgcgctgct ggtggagcgc aacagcacga
    21121 tgtcgttgca tttcacccat aaagattcga gcagttcttt caggaacgcc ggatcttcgc
    21181 cctgggttaa agatgcctga tagaacggca acatatactg gtcgaagcgc cctaaagaca
    21241 gcgaactggc gttggattcg tattgcagaa tgatgttcat gtaccagaat aactggcacg
    21301 cctgccagaa cgtctgcggc ttatgttcgg cgttatggcg agagatctcc gcaatagtca
    21361 gcagttcttc gcgacgctgg gcatctgtgc agtttgccgc catcgtttcc gccagtacgg
    21421 cgtaacgcaa aatgtgtttc tgcgaggctt ccagcagtaa cagcgctgcc tgataaaagt
    21481 gattctccgg ctgttgctga caatgttgct gcatctgtgc caccaactcc cccagcccgt
    21541 gattcaacag gcgtgggtaa tcaataataa tgtgcccctg gcctttatcc gtctggttaa
    21601 tgctgaaaat ctgcgtgctg gtcgcggctt ttacttcatc cgtcatctgc ccgttgatga
    21661 aatctttcat cgaacgtttt tcccagtacg ggaacaactc ttcacgatag atacgtttat
    21721 cttcttcgct gatagcaaag cggtcctgcg gacgcgtcgg gaattgatcc agctctttca
    21781 gcagccagta agggtccatt tccggcgaca taatcccggc gcgcggtttt acggtgcggt
    21841 taccggcaat cagttcttca tcacgaatcg aaatttcaac atgttcaagg atatacgctg
    21901 ttgctttcgc ccggcgcaat atcaccggtt cgccttcggt ttgccgatgg ctggcggtat
    21961 aaagcagcgc ccgctccagc gagatttcac gggtattggc aaacagtgca gttttgaggc
    22021 gagagatacg attcgtcatg aggaactcct gtgggaaagc gaacccgccc ttgcgagcgg
    22081 gagaagaact taagcggttt gcgccaggtg agcttcaatt ttgctcataa tggcgtcagc
    22141 acgttttact gcatcgctga tgttgacgcg cacgatggtc ttgccagcaa aacgctcttc
    22201 aaacttgatg ccgatatctt tggtcagaat caccatatca gcgctcgcca catcttccgc
    22261 agtcagttca ttttccagac caattgaccc ctgggtttct actttcactt cccagccttt
    22321 cgctttcgcg gcactttcca gcgcctctgc tgccatatag gtatgggcaa cacctgaagg
    22381 acatgcggtt actgcaataa ttttcgtcat ggctcgttcc tcaattaatt aatttcaaaa
    22441 tccaaatcca ggtcgtcttc tttttcatca gtcgaagacc catttttacg cgccagactt
    22501 ttcagcacgt tcacacaaac agccgtcacc actgctccga cagccactgc tgcgatataa
    22561 cccagcttgc cttcaaccac cggcagcaca atcagaccac cccagcctgc atagcactgc
    22621 gcaccgacca gcgccgcagt tactgcacca caaactgaac cgaccatgat ggaaggaatg
    22681 acacgcagcg gatcggcagc ggcgaaagga atcgcccctt cagtaacgcc aacacagccc
    22741 atcaccagtg ccgctttgcc ggtttcgcgc tcttctgcgg agaaattttt acgaccaatc
    22801 agcgtcgcca gccccatccc cagcggcggg atacaaatac ccacggcagc gatgaccacc
    22861 acggtataaa caccctgagc aacgcaaatc agcatgaagg cataggccac tttgttaacc
    22921 ggaccgccca tatcgaacgc cagcatcaga cccatgatca ccgccagcat aacaatgctg
    22981 ccctgctgca tcccctgaag ccactgagtc aggctgttgg tcaacgcccc tacaggctcg
    23041 cccaagcccc acatcatgat gcctgcggta atcagtgtgc cgacgatagg aataatgaag
    23101 ataggcatca ccgagcgcag aactttatgc accggaattt tcttcaggta atgcaccacg
    23161 atgccgccga taatcccggc gatcagtgca ccaaagaagc ccgcaccaaa gctgttacca
    23221 acccaggcac cgatagcgca cggagccagc gcagaacgct ctgcaatgga gtaaccaatg
    23281 taagcggcga ggaaaggcac catcagcgtc aaacctgcaa cgccgatatc aaacagtttt
    23341 ttcaaattcg gatcggctac ggcatccggc actgcgcctt tgccatacaa catgacggaa
    23401 accgccagca aaataccgcc cgataccacg aagggaatca tgtgtgaaac gcccgtcatt
    23461 aaatgctgac gggtattttt caggatctgc accaactcat tcataagctg ctcccgggct
    23521 tgcgaaagcc catgtccata tttttgatgg aggaaacaat aggcaatcgc aggcggagca
    23581 aacagtggat aaaaaggcca tttactggac ttttgtaata tcagtacaaa aatgcgatcc
    23641 gcctcataac ttgcgataaa ccggaagcat gccgcaaaac ggcgtcttgt gagacgcgcc
    23701 gcaaattgac ggttacatta catttgtcca gcttttggca gatttgtcac atggcaggcc
    23761 gttgccgtgc gctcttatcc tgtaaacaga aaaccgtacc gggagagagg caatggccct
    23821 gattgtggaa tttatttgtg agctacctaa cggcgtacat gcgcgtccgg caagccacgt
    23881 tgaaacgctg tgtaatactt tttcatcaca aattgagtgg cataacctgc gcactgaccg
    23941 caagggcaac gccaaaagcg cccttgcgct gattggcacc gatacgctgg cgggcgataa
    24001 ctgccagtta ctgatttccg gggccgacga acaggaagcg caccagcgtt taagccaatg
    24061 gctgcgcgat gaattccccc actgcgatgc gccgctggcg gaagttaaat ctgacgaact
    24121 ggaaccactg ccggtttcac tgaccaatct gaatccgcaa attatccgcg cccgcaccgt
    24181 gtgcagcggt agtgcaggcg gcattctgac gccgatctct tctttagatc tcaatgcgct
    24241 gagtaatctt cccgcagcca aagacgttga cgccgagcaa tccgcactgg aaaatggcct
    24301 gacgctggta ctgaaaaaca ttgagtttcg tctgctggat agcgacggtg ctaccagcgc
    24361 gatactggaa gctcaccgat ccctggctgg cgatacatct ttacgtcagc acttactggc
    24421 aggcgtcagc gagggattaa gctgtgccga agcgattgtc gccagtgcga atcacttttg
    24481 cgaagagttc gcccgttcca gcagcagcta cctgcaagaa cgtgccctgg acgtacgcga
    24541 cgtctgcttc cagttactcc agcaaatcta cggtgagcaa cgcttcccgg caccgggcaa
    24601 actgacgcag cccgccattt gtatggctga tgaactgact cccagccaat tcctcgaact
    24661 ggataaaaat cacctcaaag gtctgttact gaaaagtggc ggcaccacct cacataccgt
    24721 aattcttgcc cgttcgttca acattccgac actggttggt gtagatattg atgcccttac
    24781 tccgtggcag catcaaacga tttatatcga cggcaacgcc ggggcgattg tggttgagcc
    24841 aggggaagcc gtggcccgtt attatcagca agaagcccgc gtacaggacg cccttcgtga
    24901 gcaacagcgt gtctggctga cccaacaagc ccgtaccgct gacggtatcc gcattgaaat
    24961 tgccgctaac atcgctcact ccgtggaagc gcaggccgca ttcggcaatg gtgcggaagg
    25021 cgttggtttg ttccgcactg aaatgctcta tatggatcgc accagtgcac cgggcgaaag
    25081 cgagctgtac aacatttttt gtcaggcgct ggagtctgcc aacggacgca gcattattgt
    25141 gcgcactatg gacattggcg gcgacaaacc cgttgattat ctgaacattc cggcagaagc
    25201 taacccgttc ctcggttatc gcgctgtgcg tatttatgaa gaatacgcct cgctgttcac
    25261 cacacaacta cggtcgatcc tgcgcgcctc cgctcacggc agcctgaaaa tcatgatccc
    25321 gatgatctcc tcaatggaag agatcttatg ggtgaaagaa aaactggcgg aagccaaaca
    25381 gcaactgcgt aacgaacaca ttccgtttga tgagaagatc cagcttggca ttatgctgga
    25441 agtaccgtcg gtgatgttca tcatcgatca atgctgcgaa gagattgatt tctttagtat
    25501 tggtagtaat gacctgacgc aatatctgct ggcagtggat cgcgataacg ctaaggttac
    25561 tcgtcactac aacagcctga atccggcatt cttgcgggcg ctcgattacg ccgtgcaggc
    25621 ggtgcatcgc cagggcaaat ggattggtct gtgcggtgag ctgggagcga aaggttccgt
    25681 gctgccgttg ctggtcggct tagggctgga tgaactcagc atgagcgcac catcaattcc
    25741 ggcggcgaaa gcgcggatgg cgcaacttga tagccgtgag tgccgcaagt tgctcaacca
    25801 ggcaatggcc tgccgtactt cgctggaagt ggaacacctg ctggcgcaat tccgcatgat
    25861 ccaacaggac gcaccgctgg tcaccgccga gtgcatcacg ctggaaagtg actggcgcag
    25921 caaagaagaa gtgctcaaag gcatgaccga taacctgctg ctggcgggcc gctgccgcta
    25981 tccgcgtaaa ctggaagccg acttatgggc gcgcgaggcc gttttctcta ccggtctggg
    26041 ctttagtttt gccattccac acagcaaatc agaacacatt gagcaatcca ccatcagcgt
    26101 ggcgcgtctg caagcgccgg tgcgctgggg cgatgatgaa gcgcaattca tcattatgtt
    26161 aaccctgaac aaacacgctg cgggcgatca gcacatgcgc attttctcgc gcctcgctcg
    26221 ccgcatcatg cacgaagaat tccgtaacgc gctggttaac gccgcctctg ccgacgctat
    26281 cgccagcctg ctgcaacatg aactggaact gtaaaaggaa acatcatgga actgtatctg
    26341 gacaccgcta acgtcgcaga agtcgaacgt ctggcacgca tattccccat tgccggggtg
    26401 acaactaacc cgagcattat cgctgccagc aaggagtcca tctgggaagt tctgccgcgt
    26461 ctgcaaaaag cgattggtga tgagggcatt ctgtttgctc agactatgag tcgtgacgcg
    26521 cagggtatgg tggaagaagc gaagcgcctg cgcgacgcca ttccgggcat tgtggtgaaa
    26581 atcccggtga cttccgaagg tctggccgcc attaagatgc tgaaaaaaga gggtattact
    26641 acgcttggca ccgcagtgta cagtgctgca caagggttac tggccgcgct ggcaggggca
    26701 aaatacgttg ctccgtatgt taaccgcgta gatgcccagg gcggggacgg cattcgtacg
    26761 gttcaggagc tgcaagcgct gttagaaatg cacgcaccag aaagcatggt gctggcggcc
    26821 agctttaaaa cgccgcgtca ggcgctggac tgtttactgg caggatgtga atccatcacc
    26881 ctgcccttag atgtagcgca acaaatgctc aacacccctg cggtagagtc agctatagag
    26941 aagttcgaac acgactggaa tgccgcattt ggcactactc atctctaaag gagcaattat
    27001 ggaccgcatt attcaatcac cgggtaaata catccagggc gctgatgtga ttaatcgtct
    27061 gggcgaatac ctgaagccgc tggcagaacg ctggttcgtg gtgggtgaca aatttgtttt
    27121 aggttttgct caatccactg tcgagaaaag ctttaaagat gctggactgg tagtagaaat
    27181 tgcgccgttt ggcggtgaat gttcgcaaaa tgagatcgat cgtctgcgtg gcatcgcgga
    27241 gactgcgcag tgtggcgcaa ttctcggtat cggtggcgga aaaaccctcg atactgccaa
    27301 agcactggca catttcatgg gtgttccggt agcgatcgca ccgactatcg cctctaccga
    27361 tgcaccgtgc agcgcattgt ctgttatcta caccgatgag ggtgagtttg accgctatct
    27421 actgttgcca aataacccga atatggtcat tgtcgacacc aaaatcgtcg ctggcgctcc
    27481 tgcacgtctg ttagcggcgg gtatcggcga tgcgctggca acctggtttg aagcacgcgc
    27541 ctgttcccgt agcggcgcta ccactatggc gggcggcaag tgcacccagg cggcgctggc
    27601 actggcagaa ctgtgctaca acactctgct ggaagaaggc gaaaaagcga tgcttgccgc
    27661 cgaacagcat gtagtaaccc ctgcgctgga acgcgtgatt gaagcgaaca cctatttaag
    27721 cggtgttggt tttgaaagcg gcggactggc ggcggctcat gcggtgcata acggcctgac
    27781 cgctatcccg gacgcgcatc actattatca cggtgaaaaa gtggcattcg gtacgctgac
    27841 gcagctggta ctggaaaacg cgccggttga ggaaatcgaa accgtagctg cacttagcca
    27901 tgcggtaggt ttgccaataa ctctcgctca actggatatt aaagaagatg tcccggcgaa
    27961 aatgcgaatt gtggcagaag cggcatgtgc agaaggtgaa accatccaca acatgcctgg
    28021 cggcgcgacg ccagatcagg tttacgccac tctgctggta gccgaccagt acggtcagcg
    28081 tttcctgcaa gagtgggaat aacctactcc aaactcccgg cttgtccggg agtttgaacg
    28141 caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa tctctgcaat atattgaatt
    28201 tgcgtgcttt ttaggccaga taaggcgttc acgccgcatc cggcactctg tgccagtaat
    28261 ctgaaactcc tacgttgtcc aggagttttt tatccgcttc tggacatttt ctctacagaa
    28321 caatcgttag cccggaaggt cgaatctgtg actcttattt tcacggacga accagatgaa
    28381 agcttcactg gcgctgctca gcctgctaac ggcatttacc agccactcct taaaatctcc
    28441 cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc aaccttgcca tcgcagacgg
    28501 tgccagacag caaatcggca acacgctatt ttacgatccg gcgtatgtgc ggcttaccta
    28561 tcctggcggt gatgtcccgc aagaacgcgg tgtatgttcc gatgtggtga tccgtgcatt
    28621 gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa gatatggcga agaattttgc
    28681 cgagtacccg caaaaatgga agttaaagcg cccggacagc aacatcgatc accgtcgggt
    28741 gcctaatctg gaaacctggt ttagccgcca caacaaaaca cgccccatca gtaaaaatgc
    28801 cggtgactat caagcgggcg atattgtctc ctggcgatta gataacgggc tggcgcatat
    28861 tggggtggtg tcagatggct tcgcccgcga cggtacgcca ctggtgatac ataacattgg
    28921 cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg atggtggggc attatcgtta
    28981 ttttgttaaa taagcgatcg ccccacatga agaaaaaatt cagatgcttt ttacactgac
    29041 agcttctttt ttcttacggc tcaccagcgc cagtgccagc acaatcagca caataccgct
    29101 accttcaacg gcccccggat tctcaccgag cagccaccag gaaaagagta cgccgcaaac
    29161 gggaacggcc agcgtgctta agctggcaat actggcaggc aagtttttca atacaaacaa
    29221 ccataagctc cacgccagcg ccgtcgccag aatcgcactg taggccagcg cccagaacac
    29281 ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa gcgaccacac tcatcaccag
    29341 cgccgcatag agcatctgcc aggatgttaa cgataataaa tccacgcgcg gatgacgggt
    29401 gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta acaccggaga ggattgccag
    29461 catggcactt ttcatcgaag agaaatcgag ttgccacggc tgcaacacca aaaataagcc
    29521 gaaagcggca atcagaatcg cgaaatattg cccacgtcgc aggcgttcac cgagaaacaa
    29581 cgcggcgaaa atcaccaccc agaacggcat ggtatagctc aggagcgcca ccttccccgc
    29641 acctccgctg accaacgccc actgcgccag accaaccatc ccgcaggttt gtaacagggc
    29701 aatggctaag gtgtatttaa acggcgtcgg gtgaattccg cgaccacgta ataaaaggac
    29761 gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg gtaaagtcga aggcaccgat
    29821 gtaactggtg acttgcttca tgaaaatcca gctataactc cagataagtg tgagcacaac
    29881 caggccactg attgccagtg ggttgctctt tcctgcggca gacatgagag atccagtgtg
    29941 tagttttcct gtggacacta tacgccggta aagaaacttc agcgactgcc tgaaagatag
    30001 tcactgaaga aggtcagatt acagcaggtc gaaacggtcg aggttcatca ctttcaccca
    30061 tgccgctacg aagtctttaa caaacttctc gtgggcatcg ctgctggcgt aaacttccgc
    30121 taccgcacgc aggacagagt tagaaccaaa taccagatcc gcacggctgg ctgtgtattt
    30181 cacttcgccg gtttcgcggt cacgtccttc gaacagctct ttcgattcgt cagtcgcttt
    30241 ccactcgtaa cgcatatcca gcaagttcac gaagaagtca ttgctcaata cgccaacgcg
    30301 gtcagtgaag acgccgtttt tgctgctatc gaagttggca cccagtacac gcatcccgcc
    30361 caccagcgca gtcatttccg gcgcggtcag cgtcagttgc tgtgctttgt cgatcagcag
    30421 tgactcggtg gtggaaacgt ccagacgagc gcgatagtta cggaaaccgt cagcaattgg
    30481 ctccagcagc tcaaacatct caatgtcagt ctgatcctga cgcgcatcaa cgcgacccgg
    30541 cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg gctttctcaa caccaaccac
    30601 accagccagc acgatgatat ccgccagcga ggctttacca gactctttct ggattttctc
    30661 cagaacaggc agagcacgaa cggctgcggc gttcacatcc cagtcgcgct gcggcattaa
    30721 tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca ccacggaagg tagaagcaga
    30781 tgcccaggcc accgatacca gctcactaac agacagacca gaatccgcaa tcgcgaattt
    30841 cagatcgata atgtcctgct cggtcgggtt gtagatcggc tgcggcagcg gatcttgcca
    30901 gatcagatct tctttcggca cttccggccc gatgtagcga gatttcggcc ccatatccct
    30961 gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg aacgcctgcg gatcgttgag
    31021 gaaacgacga gagatcttct cgaactcagg atcaaaacgc agcgtcaggt cggtcaccag
    31081 cattgtcggt ttacgtttct tcgacggatc gaacggatcc gggataattt ccggtgcgtc
    31141 taccgcttcg aactggattg ctccagccgg actgcgggtc tgtacccact catatttgaa
    31201 caggttctcg aagaaaagtt gctccactgg gtcggcgtct gggtccagac aacttccaga
    31261 ccagaggtaa tggcatctgc gccaacgccg ctgccgtaag tgctcgccca gcctaaacct
    31321 tgttcttcaa tcggtgcagc ttctggatca ggacctacat ttgatgtcgg accggcaccg
    31381 tgagttttac ccagcgtatg accacccgca atcagcgcca cggtttcttc gtcgttcatg
    31441 cccatgttgc cgaaggtcgc acggatagct gctgccgcag aaagcggttc gccgctgtga
    31501 tccgggcctt ccgggttaac gtaaatcaga cccatctcgg ttgcacccag cggtgctttc
    31561 gccagcgctt ccggatgacg gtgagtcagc caggcttttt catcacccca gttaacatcc
    31621 agatccggtt cccagacgtc ttcacgaccg gcaccaaaac cgaaggtacg gaagccggag
    31681 ttttccagcg ccacgttacc tgcgaggata aacaggtcgg cccaggagat tttctgacca
    31741 tatttctgtt tgattggcca caacaggcga cgcgctttat cgaggcttac gttatccggc
    31801 caggagttca gcggtgcaaa acgttgctga ccacgacccg cgccgccgcg tccatcgatt
    31861 gaacggtaag tacctgcgcc gtgccaggcc atacgaataa acagaccggc gtagctgccc
    31921 cagtcggctg gccaccacgg ctgagattct gtcagcaggg ctttcagatc ttttttcagg
    31981 ccgtagtaat ctaatttgct gaattctttg cggtagtcaa agtcctcacc cagcgggtta
    32041 gaacgattag aatgttggtt taacaggtca acacggagtt gatttggcca ccagtcgcga
    32101 gtggttgtgc ccgcccccgc gctctggtcg tgaccgccct gatggaacgg gcatttgcca
    32161 gtggctgtcg tgttatggat atcgtctgac gtgctcatca atgtgctccc ctctacagtg
    32221 ttaccgttac gatacacagc gttagagaga agttataatt gaattaatcc acggatgcga
    32281 tagttgagat cttacatatt gttggttaaa gagatgtagg tcaaattgat cgtaattaag
    32341 cttttttgat tatgtccgaa ttcggacaaa tgctttataa aaaagggttt tagcttcata
    32401 tccttcaagt aatgagggag aaaacaaggc tgacaacctt attttcccgc cctcatttcg
    32461 aggcagcatt ttgtgctctg tttaaaattt gtgatcactg tgtgattttc acaaaagcca
    32521 cactatttat aaaccaggtc gaacacccag cgtatggcaa atcgcgtaac tcatttcagc
    32581 gcggttaagc gtatagaagt ggaaatcttt cactccttca cggcttaaaa tcttcaccat
    32641 atccatggca atattcgcgc ccaccagttt gcgggtttcg gcatcatcat ccaggccgtc
    32701 gaacatttgc gccatccacg ccggaatacg cacgttggtc atatcggcaa atttcttcgc
    32761 ctgtttaaag ttagataccg gcaaaattcc cggaataatt tccacatcaa tgcccgccga
    32821 tacacagcgg tcacgaaaac gcaggtagct ttcgacatcg aagaagaact gagtaatcgc
    32881 gcggttggct ccggcatcga ctttgcgttt cagattaagc aaatccgcct gagcgctttt
    32941 tgcttccggg tgaacttccg gatacgccgc cacggagata tcgaaatctg ccacttcttt
    33001 taatagcgtc accaggtcag aagcatacat ttctggctta ccacttcccg gcggcagatc
    33061 gccacgcagc gccacgatat gacgaatacc gttattccag tagtcgcgtg caatggtgcg
    33121 caactcgtcg ggcgtcgcat caatgcaagt aagatgcggt gccgcttcca gaccagtgcg
    33181 atctttaatg cctttaataa tgctgtgcgt acggtcgcgc tcgccggagt tcgcgccata
    33241 ggtcaccgat acaaacttcg gtttcaggct gctaaggcga tcgatggagt tccacagggt
    33301 ctgctccatt tcactggtac gcggcgggaa aaactcgaac gaaacgttaa tctgcccctg
    33361 gacttctgcc aggctctgat tcagggcatc ccgctggctg gcgtgaaaaa agctcatacc
    33421 ttacctcatc aatcgcttgt agttatatgt tgtgttgtgc acatctatcc gtttagacgt
    33481 ccagatgtaa agatgaagga aaagccgaag ggcgtcaacc gaaaaatgac caatgatggt
    33541 gaggaaaaat cagggaagat gaaaaaactt caggatggta agaaaaagaa aatccggtat
    33601 gcaaagcaaa ccggactgca aatataaact aaccatgtac ttattgcttc ttgccggatt
    33661 cgtcgtaaac gcttaatccg gcctaatcct atgataattc aatatatttc attagaaacc
    33721 ataaacccga aaacatgagt accgggcatt attaaatttc tgaaattaca acaactgtgc
    33781 cagccggttg atatccgact gaatcgcccc ggcggtgacg tcgcgccccg cgccaggccc
    33841 acggatcacc agcgggttgt cgcgatacca gcggctttcg atggcaaaga cgttatcgca
    33901 cggcagcagt gatgccagcg gatggtcttc acgcaccgcc tccacgccca cacgcgcttt
    33961 gccgttggca tcgaaacgcg ccacgtagcg cagcaccagc cccatttcgc gggccgcttc
    34021 cagccgttgc accatctgct cgttaagttc atcgccattt tcaaagaaat ggtcgatgct
    34081 gccgccttcg caatgagcag gcaccagcga ttccacacgt acctggtccg gttcaatgtt
    34141 gtaacccgct tcacgcgcca gaattaccag cttgcgcatt acgtctttgc cggagaggtc
    34201 gtcacgcgga tcaggctcgg ttaagccctg ctgccacgcc tgatccacca gctcggtaaa
    34261 cggcacgcta ccgtcgaatt gcaggaacag ccaggagagc gtgccggaga agatcccgct
    34321 gatcgacaaa atagtatcgc cgctgtcgat cagatcgcgc acggtgtggt tgatcggcaa
    34381 gcccgcaccg acggtggcat tgtacagcca gtgacgcccg gttttttcga agccgtcgtg
    34441 gatctggcga tatttattgc tgtcgctggc tcccgccagt ttgttggcgc tgataacgtg
    34501 gaaaccgtgg ctggcgaaat caagatactg atcagcaagc tgctggctgg cggtaacgtc
    34561 cagcaccact aaatcatcat acggatgggc gcgcatccac aggaacaacg actcttcatc
    34621 ctgctcaacc gcttcatcgt tgaagaaggc taacgcgcgg ctggcgtcca gcccgtcata
    34681 gctcaacagg ctgcggcggc tgtccaccac acctgccagc acaaactcaa agccggtacg
    34741 tgccgaaagc gtgctctgct cacgggcgaa cagttccagc caacgggaac cgatattgcc
    34801 cttaccgaac aataccaggc cgatgcgttt ttctgcgcgg aagacggact gatgcagccc
    34861 ctgaatcagg ctttcggtcg ggccggtgcg cagtactgcc accaggctga tgccgtcatc
    34921 ggactgccag gtaaattcga ccggctggcc tttcagttgc tgccagaagc ggtggcaatg
    34981 cagcgggtta cgggtgacgc ctgcaccgac catcgccacc agcgccagcc cctgacgcag
    35041 gcgcagttcg ccaggtaatc ccgcttcgtc gaggattttc agcgcactgt cggccacttc
    35101 tgaggtgtag caaaattgca gcaactggcg atcgttatgt acgccaaccg ccagcgggcg
    35161 tacctgcgcg cgtttcagga tttggtcgat ctctttatgc gccagtttga aatcctgact
    35221 ggcgggcacc tgaaactcaa tcaaacagac atcatcgtgg ctggtgacaa tacgcgcacc
    35281 agtaccggag gccagcacgc gttcaatgcg cgtggaacct tgatccggcg tgtagctaca
    35341 gcgcagttgc aggtcgattt cgctgccaga aaccggctgt aaagtacggg cgtgaagaac
    35401 gggagccgcc aggcgcgcca gttcgctggc ctcatccaga cgcagcaacg gcagcaggca
    35461 ggcatctttc actttacgcg ggtcggcact gtataccccg gcgacgtcgc tccagatggt
    35521 tacgcgagaa acacccgcca gcgcaccgat ttgtgtcgcg gaatagtcgg aaccgttacg
    35581 ccccagcagc accgtttcac cggcgttgtt gcggctgata aatccggtca ccaccagacg
    35641 tttgcccgga tgttgcacca gcagctgttg cagcaacggg taagaaagcc cttcatcaac
    35701 ctgcggttgt gcggcgcgtt cagcgcgtaa aaactcgcgg gcatcaagcc aggccgctgg
    35761 cagcccttgt tgattaagta ccgcagacat caggcgtgcc gaccatactt ccccgtggcc
    35821 caccacttcc gcatacactg cgtcgttaat accgctgtcg agcagcgccg ccaggcgctc
    35881 aaggtcgctg acaaaagcgc gaatgaggct atcagcttct tcagcgggta gcagaccgct
    35941 aatcagatcg cactgatagc gacgcagcgt ttgttgaacc tgatgcgcag agagacgatc
    36001 ggtctggctt agtttcaacc agttaatcaa ctggttagtg gtgctaccgg cggcggaaac
    36061 caccatcata tcgtcaggct gagagtactc cgccataatg cccgcaacac gcaaataaca
    36121 cttcacatca gccagactac tgccaccaaa tttatgcagt tgacgacctt tcgcccctgc
    36181 ctgcgcaatc acactcattt ttaccccttg tttgcagccc ggaagccatt ttccaggtcg
    36241 gcaattaaat cttcgccatc ttcaataccg gtggagatac gcagcagcgt ctcggagatc
    36301 ccggcggcag cacgcgcttc tggtgccatg cctgcatgtg tcatggttgc ggcgtgagag
    36361 attaaacttt ccactccccc taatgattcc gccagcgtaa acaacgacag cccgcccagg
    36421 aaacgacgca gcgtctgctc atcgccatcc agttcaaaac tcaacattgc gccaaagcct
    36481 ttttgctggc gcgcggcaat ttcatgcccc tgattttccg acagcgacgg gtgatacagt
    36541 tttttcacca acggctgggt ttgcagatat ttcacaatcg cctgcgcgtt gcgctgggcc
    36601 agctccatac gcggcaccag cgttcgcaac ccgcgtagca gcagatagct gtcaaacgcg
    36661 ccgcccgtca cgccaatatt gtttgtccac caggcaagtt cagtgacaac gtccgggtct
    36721 ttagcaatca ccacgccagc caccacgtca gagtgaccgt tcagatattt cgtacatgaa
    36781 tgcaacacca gatcggcacc caatgccagc ggattttgta atgccgggct taagaaggtg
    36841 ttatccacca cgctcaccgc cccgacttcc cttgccagat ggcagatttt cgcaatatcc
    36901 acgatgcgta acaatggatt acttgggctt tctaccagta ccagtttggg tttttctgcc
    36961 agcgctgccc gtaatgcctg ttcatcgcct tgatcaacaa acaacacgcg atagcaaccg
    37021 cgtttcgcca gactgtcgaa caggcgatag ctaccgccgt agcagtcgtg cggcgcaacc
    37081 agcagatcgc caggtttcaa aaagacggtc gttaccaagt gaatcgcgga catgccggta
    37141 ttagtaagta ctgcgccagc accgccttcc agttctgcca gcgcacgctg aaccacatcg
    37201 cgcgttgggt tgccgcgacg cgagtaatca tgcgcgcgcg gttcattaaa tccggtaaag
    37261 ttataggtgc tggaaagatg gatgggaggg acaacgcaac catactgttc gtcgtcattt
    37321 aacccgctac gcactgcgat ggtggcctgt ttacgcgtca tgtgatgaag tttcctgggc
    37381 tttgtcggtg aaatgtcagg caccagagta aacattgtgt taatggacgt caatacatct
    37441 ggacatctaa acttctttac gtatagattg agcaaatccc aaatagccgt taaaattata
    37501 tgcattatca cgccgacagg tgcattacac gatgtcacgg taacgcctgt acggtaaact
    37561 atgcgggttt acggtcagta cccacataaa ttgtgtggtc tggtctcaat ttattgacga
    37621 agaggattaa gtatctcatg gctgaatgga gcggcgaata tatcagccca tacgctgagc
    37681 acggcaagaa gagtgaacaa gtcaaaaaga ttacggtttc cattcctctt aaggtgttaa
    37741 aaatcctcac cgatgaacgc acgcgtcgtc aggtgaacaa cctgcgtcac gctaccaaca
    37801 gcgagctgct gtgcgaagcg tttctgcatg cctttaccgg gcaacctttg ccggatgatg
    37861 ccgatctgcg taaagagcgc agcgacgaaa tcccggaagc ggcaaaagag atcatgcgtg
    37921 agatggggat taacccggag acgtgggaat actaaagcaa aaaagagcgg cgcggagtgg
    37981 aatcgcctga tgcgctacgc ttatcaggcc tacgtcatat tgcaatttat tgaatttgca
    38041 cgaacttgta ggccggataa ggcgttcacg ccgcatccgg cataaacaac gagcacgttg
    38101 tctgcgaccc accgcttttt atacatggac gtttaactat gaaaaacagg ctgctgatcc
    38161 tcagcctgct ggtttctgta cctgcctttg cctggcagcc acaaaccggc gacatcatct
    38221 ttcagatctc tcgctcatcg caaagtaaag cgatccaact ggcgacccat tccgattata
    38281 gccacaccgg tatgctggtg atacgcaaca aaaagcccta cgtttttgaa gcggtcggcc
    38341 cggtgaaata caccccgctc aagcagtgga tcgcccatgg tgaaaagggc aaatacgttg
    38401 ttcgccgcgt tgaaggcgga ctgagtgttg aacaacagca aaaactggcg caaacggtaa
    38461 aacgttatct cggtaaaccg tatgatttca gcttctcatg gagcgacgat cgccagtact
    38521 gttcggaagt ggtgtggaag gtttaccaga acgcgctggg aatgcgcgtg ggcgagcagc
    38581 agaagttgaa agagttcgat ctcagcaacc cgctggtcca ggctaagctc aaagaacgct
    38641 acggcaaaaa tattccgctt gaggaaacgg tagtttcacc acaagccgtt ttcgacgcgc
    38701 cacaactcac cacggttgcc aaagagtggc cgctgttttc gtggtaatgg atgatgaaaa
    38761 aaataattcc cgccgtagtc tgtttgttgc tttgtagttg cacgaccttt ccgcaaactg
    38821 tcgctccggt aaacggtgat gcctccaatt agttgaatta aggatataat actcgctttt
    38881 aaggttgttt catggtcaca gttaaccttc attgcccccg ttgccagtcc gttcaggttt
    38941 accgtcacgg gcagaaccca aaaagacatg atcgattccg cggccgtgac tgtcaccggg
    39001 tatttcagct cagttactgt tacgagcccc gtaaaccggg agttaaagac caaatcgctg
    39061 aaatgacttt caacggtgcc ggagttcgcg atactgcccg tacgctgaaa atcggcatta
    39121 atacagtcat ccgaacttta aaaaactctc gccacgaaga ataacgtcat ccccggtcgc
    39181 tcatgctgat gtggcactca tctgcgaact tagtcagcaa tggagctttg tcggcagtaa
    39241 agcgcgacag cactggctct ggtacgtgta caatacgaaa actggtggtg ttctggccta
    39301 cacttttggc ccccggactg acgaaacctg tcgtgaattg ctggcgctgc ttaccctttt
    39361 accatcggca tgttgaccac tgacgaatgg ggtagttaca ccagagagtt accgaaggaa
    39421 aagcatctga ctggtaagat ttttactcag cgcattgagc gcaacaatct gaccctgaga
    39481 acccgaatca aacgtctggc ccgcagaacc atctgtttct cgcgctctgt agaacttcat
    39541 gaaaaagtca tcggtgcttt tatcgaaaat atatgttcta ctaattggag tcatcaccct
    39601 tctgatgagc tttccttgga tgattaccag actgaataca cagccaaaga tttttacctc
    39661 attctgctct tttttctcaa atgacatata ttatatttta catcatattc ataggcatat
    39721 gacattcccc cgcccccatt atgtcattgt gggttgatgt tgggctttca taaaaaaaac
    39781 atcttttttt gaactagcat taattatatt tactttatat attaaaatat tttctccatc
    39841 tggaaatatt ttagccacac aatataaaac agctgaatcc gaattactta aaaatatatt
    39901 atcattaccc ataactccag ccattaaatt actggagact aaagcaagaa aataacaaca
    39961 ttgtattgcc ttcattttaa ttactccctt gtctgataat tgtaactcca ttccctttag
    40021 ctttgggatt atattttaga taggtg
//
LOCUS       DAJTNA010000013        39786 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTNA010000013 DAJTNA010000000
VERSION     DAJTNA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39786
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.013"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            7..438
                     /locus_tag="OO161_000587"
     CDS             7..438
                     /locus_tag="OO161_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5461764.1"
                     /translation="MALNLASGEGYFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFS
                     VQSGPMLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            521..1423
                     /locus_tag="OO161_000588"
     CDS             521..1423
                     /locus_tag="OO161_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR5461765.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1468..3630)
                     /gene="uvrD"
                     /locus_tag="OO161_000589"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1468..3630)
                     /gene="uvrD"
                     /locus_tag="OO161_000589"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5461766.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVDTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3714..4430)
                     /gene="yigB"
                     /locus_tag="OO161_000590"
     CDS             complement(3714..4430)
                     /gene="yigB"
                     /locus_tag="OO161_000590"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5461767.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLSKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4430..5326)
                     /gene="xerC"
                     /locus_tag="OO161_000591"
     CDS             complement(4430..5326)
                     /gene="xerC"
                     /locus_tag="OO161_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5461768.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRSFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5323..6030)
                     /locus_tag="OO161_000592"
     CDS             complement(5323..6030)
                     /locus_tag="OO161_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5461769.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6027..6851)
                     /gene="dapF"
                     /locus_tag="OO161_000593"
     CDS             complement(6027..6851)
                     /gene="dapF"
                     /locus_tag="OO161_000593"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5461770.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6888..7091)
                     /locus_tag="OO161_000594"
     CDS             complement(6888..7091)
                     /locus_tag="OO161_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5461771.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7554..7874
                     /gene="cyaY"
                     /locus_tag="OO161_000595"
     CDS             7554..7874
                     /gene="cyaY"
                     /locus_tag="OO161_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5461772.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7914..10460)
                     /gene="cyaA"
                     /locus_tag="OO161_000596"
     CDS             complement(7914..10460)
                     /gene="cyaA"
                     /locus_tag="OO161_000596"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5461773.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHMAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10847..11788
                     /gene="hemC"
                     /locus_tag="OO161_000597"
     CDS             10847..11788
                     /gene="hemC"
                     /locus_tag="OO161_000597"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5461774.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLGDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11785..12525
                     /gene="hemD"
                     /locus_tag="OO161_000598"
     CDS             11785..12525
                     /gene="hemD"
                     /locus_tag="OO161_000598"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5461775.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRTLILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            12547..13794
                     /gene="hemX"
                     /locus_tag="OO161_000599"
     CDS             12547..13794
                     /gene="hemX"
                     /locus_tag="OO161_000599"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5461776.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPQGDTPAAAPQGE"
     gene            13797..14993
                     /gene="hemY"
                     /locus_tag="OO161_000600"
     CDS             13797..14993
                     /gene="hemY"
                     /locus_tag="OO161_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5461777.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15673..15834
                     /locus_tag="OO161_000601"
     CDS             15673..15834
                     /locus_tag="OO161_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461778.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15918..17153)
                     /locus_tag="OO161_000602"
     CDS             complement(15918..17153)
                     /locus_tag="OO161_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5461779.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDMLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17300..17376)
                     /locus_tag="OO161_000603"
     tRNA            complement(17300..17376)
                     /locus_tag="OO161_000603"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17340..17342),aa:Pro,seq:tgg)
     gene            complement(17419..17505)
                     /locus_tag="OO161_000604"
     tRNA            complement(17419..17505)
                     /locus_tag="OO161_000604"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17469..17471),aa:Leu,seq:cag)
     gene            complement(17526..17601)
                     /locus_tag="OO161_000605"
     tRNA            complement(17526..17601)
                     /locus_tag="OO161_000605"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17566..17568),aa:His,seq:gtg)
     gene            complement(17660..17736)
                     /locus_tag="OO161_000606"
     tRNA            complement(17660..17736)
                     /locus_tag="OO161_000606"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17700..17702),aa:Arg,seq:ccg)
     gene            complement(17839..19224)
                     /locus_tag="OO161_000607"
     CDS             complement(17839..19224)
                     /locus_tag="OO161_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5461780.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19415..20155)
                     /gene="rffM"
                     /locus_tag="OO161_000608"
     CDS             complement(19415..20155)
                     /gene="rffM"
                     /locus_tag="OO161_000608"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5461781.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20158..21510)
                     /gene="wzyE"
                     /locus_tag="OO161_000609"
     CDS             complement(20158..21510)
                     /gene="wzyE"
                     /locus_tag="OO161_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5461782.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21507..22586)
                     /gene="rffT"
                     /locus_tag="OO161_000610"
                     /gene_synonym="wecF"
     CDS             complement(21507..22586)
                     /gene="rffT"
                     /locus_tag="OO161_000610"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5461783.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPG
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22583..23833)
                     /gene="wzxE"
                     /locus_tag="OO161_000611"
     CDS             complement(22583..23833)
                     /gene="wzxE"
                     /locus_tag="OO161_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5461784.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23835..24965)
                     /gene="rffA"
                     /locus_tag="OO161_000612"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23835..24965)
                     /gene="rffA"
                     /locus_tag="OO161_000612"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5461785.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHIFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24970..25644)
                     /gene="rffC"
                     /locus_tag="OO161_000613"
                     /gene_synonym="wecD"
     CDS             complement(24970..25644)
                     /gene="rffC"
                     /locus_tag="OO161_000613"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5461786.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25622..26503)
                     /gene="rfbA"
                     /locus_tag="OO161_000614"
     CDS             complement(25622..26503)
                     /gene="rfbA"
                     /locus_tag="OO161_000614"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5461787.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26522..27589)
                     /gene="rffG"
                     /locus_tag="OO161_000615"
     CDS             complement(26522..27589)
                     /gene="rffG"
                     /locus_tag="OO161_000615"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5461788.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27589..28851)
                     /gene="wecC"
                     /locus_tag="OO161_000616"
     CDS             complement(27589..28851)
                     /gene="wecC"
                     /locus_tag="OO161_000616"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5461789.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28848..29978)
                     /gene="wecB"
                     /locus_tag="OO161_000617"
     CDS             complement(28848..29978)
                     /gene="wecB"
                     /locus_tag="OO161_000617"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5461790.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30034..31080)
                     /gene="wzzE"
                     /locus_tag="OO161_000618"
     CDS             complement(30034..31080)
                     /gene="wzzE"
                     /locus_tag="OO161_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5461791.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31092..32195)
                     /gene="wecA"
                     /locus_tag="OO161_000619"
     CDS             complement(31092..32195)
                     /gene="wecA"
                     /locus_tag="OO161_000619"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5461792.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32435..33694)
                     /gene="rho"
                     /locus_tag="OO161_000620"
     CDS             complement(32435..33694)
                     /gene="rho"
                     /locus_tag="OO161_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5461793.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33779..33880)
                     /gene="rhoL"
                     /locus_tag="OO161_000621"
     CDS             complement(33779..33880)
                     /gene="rhoL"
                     /locus_tag="OO161_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5461794.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34021..34350)
                     /gene="trxA"
                     /locus_tag="OO161_000622"
     CDS             complement(34021..34350)
                     /gene="trxA"
                     /locus_tag="OO161_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5461795.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34481..35746
                     /gene="rhlB"
                     /locus_tag="OO161_000623"
     CDS             34481..35746
                     /gene="rhlB"
                     /locus_tag="OO161_000623"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5461796.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEVLEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35880..37364
                     /gene="gppA"
                     /locus_tag="OO161_000624"
     CDS             35880..37364
                     /gene="gppA"
                     /locus_tag="OO161_000624"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5461797.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVDQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37411..39432)
                     /gene="rep"
                     /locus_tag="OO161_000625"
     CDS             complement(37411..39432)
                     /gene="rep"
                     /locus_tag="OO161_000625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5461798.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9870 a        10818 c        10059 g         9039 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggtatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcattcgtc tggatgcctt taaaaccagt
      121 aaagagattc agttttcggt gcagtcaggg ccaatgttat tagaaaacgg cgtaattaat
      181 ccacgcattc acccgaatgt tgcctcgcgt aaaattcgta acggtgtagg gattaataaa
      241 caagggaacg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ttgctttatc ttgatggcac aatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttaagc attgtacact cgaggaggcc agacaaactg ggactggcta
      481 cacagctgga aaatcatcag ataaatgaag ggaaacaaaa atgaaaaaaa cgctcgcaac
      541 gttattttta ttaacgtgtc tgggatcatc atccgcctat gcggataacg ctttgatttt
      601 acaaactgat tttagcctga aagatggtgc cgtctcggcg atgaaagggg tcgcattcgg
      661 cgttgaccat aatcttaaaa tctttgattt aacacacgaa attccaccgt ataacatctg
      721 ggaaggcgct tatcgcctgt atcagactgc cagttactgg ccgcaaggtt cggtgtttgt
      781 tagcgtggtc gatccgggcg taggcactga ccgtaaatcg gtagtgctga aaaccaaaaa
      841 cggtcaatac ttcgtttcac cagataacgg tacgctcact ttggtggcac aaactctggg
      901 tattgatagc gtccgggaaa tcgacgaaaa agctaaccgc ctgaaaggtt ctgaaaagtc
      961 atataccttc cacggtcgtg acgtgtatgc ctacaccggt gcacgtctgg cttccggggc
     1021 gattacgttc gaacaggtgg ggccagagct tccagcaaaa gttgtcgaac tttcttatca
     1081 gaaagcaaaa gcaacaaaag gtgaagtgaa gggcaatatc ccgattctcg atattcagta
     1141 cggcaacgtc tggagcaata tcagcgacga gttgctgaat caggcagaaa tcaaactcaa
     1201 cgacacgttg tgtgtgacca tttccgaagg ttcccagcaa aaatacgcag ggaaaatgcc
     1261 gtatgtcgca agcttcggcg atgtgccgga aggccagccg atggtgtatc tgaacagctt
     1321 attgaatgtt tccgtggcgc tgaatatgga taatttcgcg cagaaacatc aggttgcgtc
     1381 tggtgctgac tggaatattg atgtgaagaa gtgcgctaag taaggtgctg atgcggaatg
     1441 cggtgtggcg caccgcatcc cgcaacgtca caccgtctcc aaccgggcat aagccgccac
     1501 cagccattta ataccctggc cctggaatgc cacctgcaaa cggctatgtt caccgctgcc
     1561 ttccatattg acgatggtgc cttcaccaaa cttagcgtgg cgtacacgct ggccgagctt
     1621 gtagccgctg tcgttctcga ccatcggcgt acccatccgc tgatggctga ccgggcggct
     1681 taccgtggcg cgcaggcgca cctcttccac acactcttcc ggcagctcgc caataaagcg
     1741 cgacgggcga tggtaaacct ctttgccata cagacggcga gtttccgcgt aggtcagcgt
     1801 cagtttctgc atcgcgcggg ttacgccaac gtaggccaga cggcgttctt cttccagacg
     1861 cccgccttca tccagcgaca tctggcttgg gaacatgccc tcttccatac cgacgataaa
     1921 cacctgcggg aactccagac ctttcgccga gtgcatggtc atcagttgta ccgcatcctg
     1981 ccaggtatcc gcctgccctt cgcctgcttc cagcgccgca tgggatagga acgcctgcag
     2041 cggcattaaa tcttcgtctt cttcgttgta gctgaactgg cgcgttgccg tcaccagttc
     2101 ctctaagttt tcgatacgcg tctgaccttt ttcgcccttc tcctgctcgt acatggtacg
     2161 caggccggag tctttaatta cccggtcagt ctgtacatgc agcggcatat cggcagtttc
     2221 ctgcgctaag gcgtcgatca gttccataaa ccgctgtaag gcgctggcag cacgtccggc
     2281 gagggctttt tcctgcaaca gttcacgaca tgcctgccag agtgttaact ggcgatcgcg
     2341 cgatgtctga cgtaccacgt ccagcgtccg gtcaccaata ccccgcgttg gcgtattcac
     2401 cacacgctca aaggccgcgt cgtcgttacg gttggcaatc aggcgcagat acgagagcgc
     2461 atctttgatt tcctggcgtt cgaagaagcg catcccgccg taaatacggt acggcatact
     2521 cgcctgcaac agtgcttctt ccagcacacg cgactgggcg ttactgcggt agagaatggc
     2581 gcactcggca agcgcccctc cgttgtcctg ccaggttttg atgcggttaa ccacaaaacg
     2641 cgcttcatcg agttcgttaa aagcgcaata gagggaaata ggctcaccgt ccgcgccatc
     2701 ggtccacagt tttttaccca gacgcccgtt attgttttca atcagggcgt tagcggcgct
     2761 cagaatattg ctggtagagc ggtagttttg ctccagacga atagtttcgg caccggggaa
     2821 atcattaagg aaacgctgaa tattctccac ctgcgccccg cgccagccgt agattgactg
     2881 gtcgtcatca ccgacgatca tcactttgcc ggtgtcgccc gccagcaggc ggatccacgc
     2941 gtactgaatg ttgttggtat cctggaattc gtccaccagg atattggtaa aacgttcgcg
     3001 gtagtgttgc aggatatgcg gcttgttaag ccacaactcg tgagcgcgca gcagcagctc
     3061 ggcgaagtcc accaggcccg cgcggtcaca cgcttcctga tacgcctgat acaccttctg
     3121 ccaggtctgc tccaccggat taccgtagct ttgaatatga tgtggacgca ggccttcatc
     3181 tttctggctg ttgatgtacc acattgcctg gcgcggcggc cactgcttct cgtcgaggtt
     3241 catcgccttg attaggcgtt tgagcaggcg tagctggtcc tcgctgtcga ggatctgaaa
     3301 atcctgcggc agattggcgt ccatatggtg cgcacgcagc agacggtgcg ccagcccgtg
     3361 gaaggtgtcg acccacatgc cgccctggct ggtgcccatt agctgcccga tacgatgacg
     3421 catctccgcc gccgctttgt tggtaaacgt caccgccata atcgagtatg gcgagcagtt
     3481 ttccacgctc atcagccagg cgatacgatg caccagtacg cgcgtcttac cactgcccgc
     3541 gcccgccagc accagaaggt tgctgcgtgg cgcggccacc gcttcgcgct gtttgtcatt
     3601 aaggctgtcg agcaggtaag aaacgtccat tggcaccgcc gcgtaaaaat aggttgggcg
     3661 gagaagcgca acgccctccg ccaaaaagct gggtatatat acagatttgc tgattatatc
     3721 agcgaggtca gagatgccaa ccgggaaatt tccagatgcg gcagtaaacg gctgtcccag
     3781 gtttgcatca gatcgccatt ttccggtctg atccaacagg cctgcattcc gctgcgaatt
     3841 gccccaccca cgtcagtggt gagatcgtcc ccaacatgta agatctcgcc gatcggcacg
     3901 ttgagttttt ccgcagccag aaagtacata tcgctgaacg gttttgagcg cccgtgcggg
     3961 ccagcgcgca gcacaaactc aaaataatcc cccaaaccaa acagttccgg ctgggcgtta
     4021 ccgttggtga tcgccaccag cggccatttc ttcgacagct gttttaaggt gtcgtgagtt
     4081 tgctgcggga cgtcgattcg gctgcgccat ttggcaaagt tgatcattgc tgcgtgtgcg
     4141 cctgcactgg cttcttcggc actcagcccg gcgtcgagca tcgcttgttc aatcgaacga
     4201 aaacgccagc gcgtcacgtc gtgataaatc tcgggttccg cttcccgtac cgcctggcgc
     4261 aggcgttgca gatcttcatt ctggaagctg cgcagcgccg gatgataatt ttgcacaaag
     4321 gtaagcgcct ctcgctcggt gcgcaaaatc accggacggt tatcgtaaag ggtatcatcc
     4381 aggtcaaagg tgagcgccga gatgcgcccc aaaggccggt aaaaacgcat tatttccccc
     4441 gtttggcgcg tggatgcgcc gcatcgtaca ccgaggcaag gtgttgaaaa tcaagatgag
     4501 tatagatttg cgtggtggag aggttggcat gacccagtag ctcctgcaca ccacgaagat
     4561 cgccgctcga ctccagcata tgcgtagcga acgagtgacg taatttatgt gggtgaacgt
     4621 gattattcag cccttgtttt atgccccatt cggcaaagcg tttctgcaca ttacgcgcgg
     4681 agatacgctt gcccagtttc gacagaaaaa gcgcgtcgtc ttcgctacca aacaggtcgc
     4741 gcaaatcaag ccagtgctca atccacgcca cagcgttgcg accaatcggc aggcggcgct
     4801 ctttgctgcc tttccccatt acccacactt caccagactc caggtcgagg tgtttgatat
     4861 ccagccccac cagctcggaa agacgcagac cagcgccgta catcacttcc agcattgcac
     4921 ggtcgcgtac agcgagggga tcgttgatat caatatccag cagccgattc atatcgtcga
     4981 cgtcgatatt tttcggcaga tgacgcggcg ctttcggtgc cgaaacacct ttcgccgggt
     5041 tagctttgag ttcgttctgg ctgaccagcc agtcaaaaaa gctacgtagc gcagaaagcc
     5101 gtaacgccag acttgctgct cccagccctt tacggcgact gcgtacagca aaactgcgca
     5161 ccatcgtcac atcacattgc tgccagcttt gcaggccgtt ttcgctggca aaattgatga
     5221 tcgcctcaag ctgacgctgg tagttaagca gggttatcgg gctaagctgg cgctccacgc
     5281 tcagataacg taggtagcgt tctacatcgg tgtgtaaatc ggtcatacgc gttcaatcca
     5341 acgctccaga agctccggca acatcagcgc aatttcatga agtaactgcg ttccttgccc
     5401 ttgttgatag tgactggcat cgcgactggt aaacagcacg acacccaaat cagcatcgct
     5461 tcccagcatc gacatcgcca ccgatcccac cgctttcgct tccggtagca ccaccagcag
     5521 ctctggtccg ttaagcggcc caagatagtg ctgttcctgc cccaaacgct gaatacgcag
     5581 cggttcgaaa gactgacggc ttaatgccag atgagtgtgg ttcgacggcg cacctaagcg
     5641 ccagcgatcc ggaaacaggc gcagactcgc acctgccagg ccgagatcgc gcgcccagcg
     5701 gtgaaagcgc atcagcatat cgtcgagact gctggcggcg gtgagactgc gctgcaggta
     5761 gagtaggcga taaaacaggc cttcgttggc gatagcctgt tccatcaaca gcgccatgtt
     5821 ctcttccaga acatgaatat gattacgtgc gcgggccatg tgccactcga ccaacgaaac
     5881 ggtgccgcgt accggatgcg gcacacgtat cgcttctact gcgcgcgcat tacggataaa
     5941 aaactcagga tttttaatca gataatcgac aaccgcccgg tcatcaagct ccgtgagtgt
     6001 ttcctgcagt tcttcccctg gttgcttcat agatgaataa atccgtcgta gacatgtacc
     6061 gccgggccag tcatatataa cgggtgaccc ggacctttcc aggcgatatc aagacgaccg
     6121 ccggggagtt ccacgcgtac ttcttcggcc agcaaacctt gctgaatccc tactgcaacc
     6181 gccgcacacg cgccgctgcc gcaggcctgg gtttctcctg ccccacgctc ataaacgcgt
     6241 aaacgaatat gctcgcgctt aaccacttgc ataaaaccga tattggcgcg ctccggaaaa
     6301 cgctcgtggc tttccagaac aggaccaagc gtttctaccg ccgcggtatc gacatcatcg
     6361 acctgaatca cgcaatgcgg atttcccatc gacaccacgc cgcataagat tgtctgctcg
     6421 gcggcgcgca taatataggt cttttccgct ttgttagcgc gaaacggcac agcggaaggt
     6481 tcgaagttgg gttcgcccat atttacgcgg accagatcat catcggtgac ggtcagaacc
     6541 atccgcccgt tggcggtgct gacgcggata tcgcgcttat tggtcagccc tttcaggcgc
     6601 acaaaacggg caaagcagcg cgcaccgttg ccgcactgcg ccacttcact gccatcagca
     6661 ttgaaaatgc gatagtgaaa atccagttca ggatcatacg gcggctcaac caccagcagc
     6721 tggtcaaacc ctacccccag gtgccgatcc gccagccgac gaatcagctc cggtgaaaaa
     6781 aagacattct gcgttaccgc gtcgacgacc ataaaatcgt tgccaaggcc atgcattttc
     6841 gagaactgca tcatttactc caatcacgcg ggtacagaaa ctgactttta gtaattcacc
     6901 tgggatggac catcgccagt ggcgcgatcg tttttatccg gcaccgtgga ttgcgtttgc
     6961 gtctctaccg gtttggtcgg cggcggtgca tttttatctg caggcgggaa atagagcgga
     7021 cctttcagac cgcagcccgt caggctgaag agagtaagta atacagtgag taccttaaac
     7081 acgtttttca ttatggattg cctgtaaagt tcatcgcttt ctgctttcta tcatcgcagg
     7141 agaaggcgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt gaggaaatgc
     7201 tgcgagtata acgcttactc gtgaggattt atgttctgct ggcagtttcg tgatcggcac
     7261 ctcgcgccag gatggcttta tgcactatcg caatgtggtt tgtttgttgg cttttttagt
     7321 tccgtcagcg tgaggatgct cgctcggtaa acctgatcat aatgtttacc agaaacaggg
     7381 aaaggcgtgg tgtaactgcg ccctaatgaa gagacaaatc tttcgccaca cagagacatc
     7441 attattccgg tgacggatga atcatgccgg aacgcagcag cagaggtgca aactttgaag
     7501 ccctgacccc atgccgctat actgccgcca tcacttaaaa acaggataca acaatgaacg
     7561 acagtgaatt tcatcgcctg gctgatcaac tgtggctgac cattgaagag catctggacg
     7621 actgggatgg cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg accattacct
     7681 ttgaaaacgg cagcaaaatc attatcaacc gccaggaacc gctgcaccag gtatggctgg
     7741 caaccaaaca aggcggctac cattttgatc tgaaaggcga tgagtggatt tgcgatcgca
     7801 gcggcgaaac cttctgggat ttgctggaac aggcggcgac gcagcaggcg ggtgaaacag
     7861 tcagtttccg ctaagattgc atgccggata agcctcgctt tccggcacgt tcatcacgaa
     7921 aaatattgct gtaatagcgg cgtatcgtga tcctgattgg caggcggcat gttaccgata
     7981 gattttgtgc ggaacggaat cacctgttca cgaccatcaa ccttcacaat ctgatagaac
     8041 tgcggcaggt tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg cgatgacgag
     8101 tagaagcgac tgacgtcacg caccagttcc tctttgctgc cttcgcagtg gtgatacacc
     8161 tcaacccggt tgctttcgtc aagaatgtag atattaaagc cattctcgtc ttgcgtttct
     8221 tcgaagaaga actggatgat cccttcacta gcaaagccgt ccaccaccgc cggtaatttg
     8281 acgtgattgg tttcaacctg cactgacagg ccgtgcagtt tgttatgcga aatagcgcca
     8341 taaaactcga tggcgttctc cagcttctgt accgatacat tcaggcgttc gaagaacaag
     8401 ccccaggttt gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg gcgggtgctg
     8461 gaaaggcgca gttcaataca ctcagaaacc agttgctgca cgcgagtacg gattaacccg
     8521 cgcagatgct ggctataaca gaagacttcc acgctatctg gcggtgcggc gtcctgatgc
     8581 attttgccga gaatagtttt cagggcttcg atcatcgatt gctcgccgtt gaagtgcagc
     8641 gtacgcactt cgttccacga gttgcggtac agcaggtcaa cgctacctac caggcaattt
     8701 tgattctcgc caaagctgaa gacatccagc ttacggaaat caaaatgcac cacctgattg
     8761 cggaacgccg ctgtcgggtc atattccagg ttaacgataa tcgccatatg gcggatctca
     8821 cacgggctgt agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa atggtgcgac
     8881 acgtcggcga ccatctcctg caacttaggc aaatcgacaa tgccgttacc tttaatatac
     8941 aaacgggtgc gcgaggtcag caggccgtta aaccatgccc acgccaccag tttgttcagg
     9001 taacggttat attccagcgg ctgatggctg atgatcgact caatatttgg cgcgcggtta
     9061 tacagatacc aacctgaacg gttagcacgg cccggcggca cataaataaa ggtcagattc
     9121 ggttccgaga gatcgggtga aatctgcggg tttaccagcg tcactttacc tggtaatgct
     9181 tcaaacgcgg catacagctt acgcgtcagc acgccgatat cctgcggact ggcggagacg
     9241 ctaaggttat tgcgacgcgc aaagcggatc agattacggt agctctgcat catcgcgtcg
     9301 agcaactcgt tgtgcgcctc acgcacctga tcaatcttcc agttagcgcg gttatcgagc
     9361 attgccagac gagcttcgtc ccaaccccac tcgctcacta actggctcaa cactgcgcga
     9421 cgccagccta cgcaggcgcg ttcacggctg agcttttcgc acacttttaa atagaagcag
     9481 cgacgtacta aatccagacg ggtaaaatct tcaatcgccg tcaggtattc agtaacacgc
     9541 tccagcatca tgcagtatgg atcgagacca aacgatacaa tctcaccgtc gtgcaaacgc
     9601 tgtttgatat ctttcgccag cagacgtggg ttcgggtatt cccaggaata ggcttccagc
     9661 agcagtgttt tcagtaccgc tttgtatggg gaatcgatac tcttgtagag ctgccaaagg
     9721 ctggcaccaa agtactcttc agcagaaagc gagcttaagc caccgagatc cagccattca
     9781 tttggcgtca gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta atgctcttct
     9841 tcgtcgcacg gcaccatatt ccacagaata cgcttaccgg cgagacgcac ggcggtacga
     9901 taaaattcgt caagcagcag tatatgctgg gtggagccac aatcttcgcc ccccaggctg
     9961 ccgctttcat tatgacggaa gcggttttca tcaatcagga agaagctgac ttccacaccc
    10021 agcgaggcgg cccagctttc cagcagacta catttacgtt gtagtaactg gcgctcttcg
    10081 ctatcgagcc aggattgatg acagacccag atatccaggt cagaggaaca actttgccct
    10141 acggacgagg tgctgcccat ggtgtataca ccagtaattg gaagctcacc tttcggtgga
    10201 tcctgtactg acattccacg atacagttca agctcgttca ggtagtggcg ttgagtttca
    10261 tcaggcgtgt aaaggcaaat gcctttggga acgttaccat caaggtaacc cggcattagc
    10321 ggatggtgat agtgcaacaa tgtcggcagt agactgtaga cctgttggaa tgcaggcccc
    10381 atagcagcaa gcgcgcgatc cacacgcaat tgatttatgg catccagtct ctgtttcaga
    10441 gtctcaatat agaggtacaa gacgtatcgc ctgatttgct acccgtcatg actgtgattc
    10501 cgccaacatc aacggtaaca cgcggcattc gggatatttc gtatgtcaaa ggtaaccgtt
    10561 accacttttc gcgcctggtt tttttagttt cacgacgaaa aaatggtcta aaacgtgatc
    10621 aatttaacac cttgctgatt gaccgtaaag aaagatgcgc tacatacaag tgtagcaccg
    10681 tttattctct gtaaattcct tattacgacg gcgtgaaacg cctgtcagga tccactgcca
    10741 gacctcattt tacggtttgc gcaggcgtct acgtttcacc acaacactga catcactctg
    10801 gcaaggatgt taggatggac cacggatgat aatgacggta acaagcatgt tagacaatgt
    10861 tttaagaatt gccacacgcc aaagcccact tgcactctgg caggcacact atgtcaaaga
    10921 caagttgatg gcgagccatc cgggcctggt cgttgaactg gtaccgatgg tgacgcgcgg
    10981 cgatgtgatt cttgatacgc cgctggcgaa agtaggcgga aaaggcttat ttgtaaaaga
    11041 gctggaagtc gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcaa tgaaagatgt
    11101 gccggttgaa ttcccgcaag gtctgggact ggtcactatt tgtgagcgtg aagaccctcg
    11161 cgatgccttt gtgtccaata actatgacag tctggatgcg ttaccagcag gcagtatcgt
    11221 cgggacgtcc agtttacgtc gccagtgtca actggctgaa cgccgcccgg atctgattat
    11281 ccgctccctg cgcggcaacg tcggcactcg cctgagcaaa ctagataacg gcgaatacga
    11341 tgccatcatt cttgccgtag ccggactaaa acgtttaggt ctggagtccc gcattcgcgc
    11401 cgcgttacca cccgagattt ctcttccagc ggtaggacaa ggtgcggtcg gtattgaatg
    11461 ccgccttggt gatgcacgca ctcgcgagct gcttgccgcg ctgaatcacc acgaaactgc
    11521 actgcgcgtt accgcagaac gcgccatgaa tacccgtctc gaaggcggat gtcaggtgcc
    11581 aattggtagc tacgccgagc ttattgatgg tgaaatctgg ctgcgcgcgc tggtcggcgc
    11641 gccggacggt tcgcagatta ttcgcggtga acgccgcggt gcgccgcaag atgccgaaca
    11701 aatggggatt tcgctggcag aagagctact gaataacggc gcgcgcgaga tcctcgctga
    11761 agtctataac ggagacgccc cggcatgagt atcctggtca cccgcccgtc tcccgctgga
    11821 gaagagttag tgagccgtct gcgcacactg gggcaggtgg cctggcattt tccgctgatt
    11881 gagttttctc cgggtcgaca attaccgcaa cttgctgatc aactggcggc gctgggggag
    11941 agcgatctgt tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc acagctgcat
    12001 cagcaagatc gtaaatggcc ccgactacct gattatttcg ccattggacg caccaccgca
    12061 ctggcgctac ataccgtaag cggacagaag attctctacc cacaggatcg ggaaatcagc
    12121 gaagtcttgc tacaattacc tgaattacaa aatattgcgg gcaaacgtac gctgatatta
    12181 cgtggcaatg gcggtcgtga gctaattggg gataccctga cagcgcgcgg tgctgaggtc
    12241 actttttgtg aatgttatca acgatgcgca atccattacg atggtgcaga agaagcgatg
    12301 cgctggcaat cccgcgaggt gacgatggtc gttgttacca gcggtgaaat gttgcagcaa
    12361 ctctggtcgc tgatcccaca atggtatcgt gagcactggt tactacgctg tcgactattg
    12421 gtcgtcagtg agcgtttggc gaaactcgcc cgggaactgg gctggcaaga cattaaggtc
    12481 gccgataacg ctgacaacga tgcgctttta cgggcattac aataactctc ataacaggaa
    12541 gccataatga cggaacaaga aaaaacctcc gccgtggttg aagagaccag ggaggccgtg
    12601 gacaccacgt cacaacctgt cgcaacagaa aaaaagagta agaacaatac cgcattgatt
    12661 ctcagcgcgg tggctatcgc tattgctctg gcggcgggcg tcggtttgta tggctggggt
    12721 aaacaacagg ccgtcaatca gaccgccacc agcgatgccc tggctaacca actgacggca
    12781 ttgcaaaaag cccaggagag ccaaaaagcc gagctggaag gcattattaa gcaacaagct
    12841 gcacagctta agcaggcgaa tcgtcagcaa gaaacgctgg caaaacagtt ggatgaagtc
    12901 caacaaaagg tcgccaccat ttccggcagc gatgctaaaa cctggctgct ggctcaggcc
    12961 gattttctgg tgaaactcgc cggacggaag ctgtggagcg atcaggacgt cacgaccgct
    13021 gcagcgttgc tgaaaagtgc agatgccagc ctggcggata tgaatgaccc gagtctgatt
    13081 accgttcgtc gggcaattac cgatgatatc gccagccttt ctgcagtttc gcaggtggat
    13141 tatgacggca tcatccttaa gcttaatcag ctttcaaatc aggtagataa cctgcgtctg
    13201 gccgataatg acagcgatgg ttcgccgatg gattccgacg gtgaagagct ttccagttcc
    13261 atcagcgaat ggcgtatcaa tctgcaaaaa agctggcaga actttatgga caacttcatt
    13321 acgattcgcc gtcgtgatga caccgccgta ccgctgttag cgccaaatca ggatatctat
    13381 ctgcgcgaaa atattcgctc tcgcctgctg gtcgcagcac aagctgtacc gcgtcaccag
    13441 gaagagactt atcgccaggc gctggagaac gtctccacct gggtacgtgc ttactacgat
    13501 actgatgatg ccaccaccaa agcgttcctc gacgaggtgg accagttaag ccagcaaaat
    13561 atctcgatgg atcttccgga aaccctgcaa agccaggcga tgctggaaaa actgatgcag
    13621 actcgcgtgc gtaacctgct ggcacaaccg gcagcaggga caacggaagc taaacctgca
    13681 cctgcacctg cacctgcacc tgcacctgca cctgcacctg cacctgcacc tgcacctgca
    13741 cctgcacctg caccgcaagg tgatactccg gcagccgcgc cgcaaggaga ataaccatgc
    13801 taaaagtgtt attactcttt gttttgctga ttgcggggat agtggttggc ccgatgattg
    13861 ccggtcatca gggttatgtg ctgatccaga ccgacaacta caatatcgaa accagcgtca
    13921 cgggcctggc gatcatattg attctggcga tggtagtgct gtttgccatt gagtggctac
    13981 tgcggcggat cttccgcact ggcgcgcaca cccgtgggtg gtttgtcggg cgtaagcgtc
    14041 gccgtgcacg taagcagacc gaacaggcgc tgctgaaact ggcggaaggc gattatcagc
    14101 aagttgaaaa gctgatggcg aaaaatgccg atcacgctga acaaccggtg gtgaactatc
    14161 tactggctgc cgaagccgcg caacaacgtg gtgatgaagc gcgcgccaac caacatctgg
    14221 aacgcgcagc ggagctggcc ggcaatgaca ccattccggt agaaatcacc cgtgtacgtc
    14281 tgcaactggc ccgtaatgaa aaccatgctg cacgccacgg cgtggataag ctgctggaag
    14341 ttacgccacg ccatccggaa gtattacgtc tggcggaaca ggcgtatatc cgcacaggtg
    14401 catggagttc tctgctggat atcatcccat caatggcgaa agcccatgtt ggtgatgaag
    14461 aacatcgtgc aatgctggaa caacaggcat ggattggcct gatggatcag gcgcgtgccg
    14521 ataacggtag tgaaggtttg cgtaactggt ggaaaaacca aagccggaaa acgcgtcatc
    14581 aagtagcgtt gcaggtggca atggcggaac atcttattga atgtgacgat catgatactg
    14641 cccagcaaat tatcatcgat ggcctgaaac gccagtacga cgatcgccta ctgctgccga
    14701 ttcctcgact gaaaaccaac aatccggaac agctggaaaa agtgctgcgc cagcagatca
    14761 aaaacgtcgg cgatcgcccg ctgttgtgga gcacactggg ccagtcactg atgaagcacg
    14821 gagaatggca ggaagcatcg ctcgccttcc gcgcagcgct gaaacaacgt ccggacgcct
    14881 acgattacgc atggcttgcc gacgcgctgg acagactgca caagccggaa gaagctgcag
    14941 cgatgcgtcg cgacggtttg atgttaacgt tgcagaacaa cccaccacag tagttccttc
    15001 tcacccggag gcaagcacct ccgggacctt tctgatgcat aaaaaaacgc ctgctcttat
    15061 tacggagcag gcgttaaaac aggtctgtat gacaacaagt gggtgcttca ctcaacgttg
    15121 tgtccatggt gtctgatgag gcataagcga catctgtcag tggacgataa gcaccgtaaa
    15181 cggctctgcg tcattccgga gtttatgagg cactaaggcg aacataagag atggaatgag
    15241 catctactcg tttattatgc cacagagaat cgggaaataa catcccttaa cacttgttat
    15301 gagataattc tgtaatcctc tttgcttcct gagtaataac ttcctgagtg aatatttaac
    15361 ctgagcttga tcctacacat acttattatg aatgataaaa ttcattcaat taataacaca
    15421 tatattaatt gccgttaaaa ctaaaaaaaa gcatcaataa tcaacgcgat ataataaacc
    15481 tgccttacat atcaactgcg ccagaggtag gattgaaaac gctctcctga ttttccaatt
    15541 cattttctgg ataaataaat aatttatttt tgtcactatt atttatgtaa tcatcctgtc
    15601 agggagaggg atctcaatta tcaatgctta attacgtcat cattttgatg acatgaaacc
    15661 ggagcatttt gcatggaatt ttcgttttca cccaaacttc ttgttgttgc tgtcgccgcc
    15721 gctcttcctc tcatggccag cgcagcagat accccgtcaa ctgccaccgc acgcaaaggc
    15781 tttgccggat acgatcaccc aaaccagtat ccacagaacg ctgcaattta ttgaatttgc
    15841 acgatcatgt aggccggata aggcgtttac gccgcatccg gcaatcaacc gcaggcggcc
    15901 acaaaatggc cgctatttta cttattcacc accagcaaat gtgcctgcat aatgtcactg
    15961 gccgggcgac cgtgcgccag caaatcagcc attgctttaa gatatggcgg tagatggcgg
    16021 aaataacgct gatacccggc acacaaataa ttcagccccg gtttgccgct ggcatcgagc
    16081 atgaagcggt gtttcgggca gcctccccag cacgctttta acacgttaca actgcgacac
    16141 tgcgccggta attgcttaaa tttatcttca ccaaacacct gctgttgcgg ggaatcgatc
    16201 atttctgcaa ttgtttgctg atgcatattc cccagccgat attgcggata aacatagtga
    16261 tcgcaggcgt aaacgtcgcc gttgtgctca acaatcaccg agcgcccaca ggttggctga
    16321 tgatggcaaa ccgcacccgg cgcaccgaca aaattggcaa acgcccattc gatattcatc
    16381 acgaaaatct tgccgacgtc gcgtttgatc cagtggtcaa aaatcgccac cagaaattca
    16441 ccaaattcat cggggcgcac agaccattcc gtcagttccc cctgaatatc accaggcgca
    16501 tgtagtttca gtccatcgct ggcagctgtt tcatcagcca ggcgctcgac caccggaata
    16561 aactggatga attcgactcc cgcatcgcac aaaaaatcat aaacctgcaa cggttgctgc
    16621 gcgctggtgc ggttgacgca gaccagcacg ttatagtcga catgatgttt ttgcagcagc
    16681 gtcagggcac gcatcaccag cttatgcgtt ggtctgccac ctttggtcac gcgatattga
    16741 ttgtggatct cagccggacc gtccagcgat aacccaacaa gaaaatgatt ttctgccaga
    16801 aatgcacacc atttatcatc gagcagcacg ccgttagtct ggaagctgtt acttatcttc
    16861 ctgccagcac cgtatttcgc ctgtaacttt acggcacggc ggtaaaaatc cagcccgagt
    16921 aacgttggtt cgccgccctg ccaggtaaaa gccacttcgt tttgcggttc gctggcagcg
    16981 atatagtgac ggacatacgc ttccagcatg tcatcgtcca tatgcgtgac tggcttttcg
    17041 cggtaaaggg attgtttttc gagataaaaa cagtagtcac agttcagatt acaatcggaa
    17101 ccactcggtt tcgccatcac atgaaaagca cgcgttggaa cctgttgcag catggtcact
    17161 ccttgcctga agtcatcctt caatacgtta agggcgaaat cataccgtgg ggcaatgagt
    17221 tattacgggc gtttattgcg aagggagggt gacgaaatgc acagaaaaca aaaaaccccg
    17281 ccgaagcggg gttcaagatt ggtcggcgag agaggattcg aacctccgac ccactggtcc
    17341 caaaccagtt gcgctaccaa gctgcgctac tcgccgaaat actgcttttt aaatttttag
    17401 ttcaattctt taacgtcgtg gtgcgagggg ggggacttga acccccacgt ccgtaaggac
    17461 actaacacct gaagctagcg cgtctaccaa ttccgccacc ttcgcattgt cacaacttct
    17521 aataatgggg tggctaatgg gattcgaacc cacgacaact ggaatcacaa tccagggctc
    17581 taccaactga gctatagcca ccactacaaa tcttgttacg cggtattact accaccgcag
    17641 ctcaagcgcc gggactaaat ggcgcgcccg acaggattcg aacctgagac ctctgcctcc
    17701 ggagggcagc gctctatcca gctgagctac gggcgcttag cgccgttgcg ggggtggata
    17761 atacggactt cccacccctc tgtctagtcc ctttttaaat aaattgcgcg tttggttacg
    17821 gtttgtgcgt tttgctgctt attcctccag tttatgcgct ttcccgtggc gattaaggcc
    17881 aaaaacttta taaatcgccg tcaccgccag cataaagatg atgccaacaa acagcgacat
    17941 acgcgtatct tcattaaagt acatgccgat caaaacgcaa atcaggaatg ccattgttac
    18001 gtagttggcc cacgggaaca ggattgagcg gaacggatgg ctggcaatcg ccgctttatg
    18061 tgcacgccga aaacgcagct ggcttatcaa tatcacaaac catggcacca tccccggaag
    18121 cacgctggca ctgtagacgt agacaaacac acgctgcgga ttgggaatga tgtagttcag
    18181 gcatgagcca attagcagaa tagcaataga tactgccaca cccgcaactg gtacgccgtg
    18241 acgggaaact ttcgccatcg ccgccggtaa ctgacggttt ttcgccagtg cgtagagcat
    18301 acgtccgcaa ctgtacatgc cgctgttaca gccagagagc gcagccgtca gcaccacaaa
    18361 gttgataatg cccgccgctg cggtaatacc gattttggca aaagtcagta cgaacgggct
    18421 gccgttgctg ccactttcat tccacgggaa gatggtgacg ataacgaaaa tcgcgcctac
    18481 gtagaaaatc aggatccgcc acagcacttt gcctacggca ctgcgcaacg tcacctgcgg
    18541 attcttcgct tcaccggcag taatgccaat cagctccacg ccctggtagg acgccaccac
    18601 aatacacagc gcggtcagga accctttcca gccacccgca aagaaaccgc catgctctgt
    18661 gagattgcta aaaccaatcg actgcccgcc attgccaaag ccaaagaaaa tcacgcccag
    18721 gccaatgaca atcatcacga taatcgtggt gactttgatc atcgcgaacc agaactcgat
    18781 ttcgccgtac aaccgcaccg ccgccagatt cgccaacgcc accagcgcca ctgcgatcaa
    18841 tgcgggtatc cactgcgcca tctccgggaa ccagaactgg acataaacgc caatggcggt
    18901 gatttctgag atccccaccg ccatccacat aaaccaataa gaccaggcgg tgagataacc
    18961 aaagaacggg ctcatataac gatgcgcata aacggcgaac gaaccggtaa ccggttcgag
    19021 gaacaacatt tcgcccattg aacgcatgat gaaaaagacg aatagcccgg cgatgatata
    19081 ggccaacaat acggatggcc cggcccattt caaggtactg gcggccccca taaacaggcc
    19141 gacgccaatg gtgcccccca gggcgatgag ttcgatatgt cgagcttcca gcccacgctg
    19201 tagctctggt ttgttatctg ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt
    19261 accggttatc gttatgggta catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg
    19321 gcaaataaag cattaaaaat ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga
    19381 gtgaccactc cgtcgcttta caaagagagg aaaatcagag gttgccggtg tagtgccagc
    19441 gtaaataacg cagcaaacga agctgacgct taatgcggct cggctgcgaa agcaggcggt
    19501 agagccactc cagtcccagc gtttgccaga ttttcggtgc gcgttttacg tgaccggtga
    19561 aaacatcgta agtcccgcca acgcccatat acagcgcatc tggatgcacc agacggcagt
    19621 cgcgcatgaa gatctcctgc tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc
    19681 tggcatgaat gcgttcaaac agcgcctgac gctgctcggg tttaaaataa ccatcctgac
    19741 tgccaacgat attcacattc cactggttgc gcagtttagc ttcagtttgc gccagcactt
    19801 caggtttacc gcccacaaga aataccggcg tcccttcttt gcctgcgcgc gccatcagct
    19861 cttcccagag atcggcaccg gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta
    19921 ctgaacgtac aacgctgatg ccatccgcat atttaaattc ggcagcgtta attaactccc
    19981 tgacctcggc gttatcttca atggtcagca ttttttcagc attaatggca accagcgttc
    20041 cctgcttaag ctgcccgtca gcaaacagat aatcgagggc gtgctgcata tcacgccaac
    20101 caatcaactg taagccacgc agcgtatagg tcggtgccgt ggtgttgtta ttcattgtta
    20161 tccttcaacc tgcgtccgga gcgatgattt tgtacgttta tgaatgagtc cggcgctttc
    20221 aaaaagccag tacaacagtt ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa
    20281 gactacgcgt gagacaaacg aatccagccc ttcacgtgcc agcacgatca tattgaagat
    20341 cgccccaaag cagaaactgt gcaatatcgc agccttatag cgattaggct cgcggttgcc
    20401 cagctcatac agccagtcga accatttgat gatcagacca accacgatcg ccccgagcgg
    20461 gatgaacaac gcgccgccca tcaccaccag tgagcctata agcgtaggcg agatcgccag
    20521 tccggagtgg ttattcagca cttccctggt aaagtagttg gctgagttca gcaccatact
    20581 cgggcgaccc ggccacagcc aggaagggat aaagacatag aaatcgcgga caattggagc
    20641 caggccctgg aagtcgatgt tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg
    20701 cgagaaggtg tcgcgagtga gatagagaaa cgtatagaac gcttcatcgc cgctcacatt
    20761 cattccatag cgttttagcg ccagccagaa catgcccaca atccccagca cgcccgccgc
    20821 cgccagcatc cacaacgaaa tccagccgcg aataatgcca ataaacagga agatagcgaa
    20881 tgcgatgatg atattggcgc gagtgccgcc gacaatcata taagtcagca agccaaaggc
    20941 gaccgtgctg acgaggaaaa acagccacgc tttgctatcc tggcgcagaa agtagaccac
    21001 cagcatcgcc gggatgaaaa agtaaaagaa gcgttttaac gccacgccgg agacttcact
    21061 ggagaagatc tgactgtagg agttaagccg gaacagcaaa aagccgttgt gcatgaagaa
    21121 gatgccgacg cttaccagcg cgatgcccat caggatcacc cacgtaagat tggtctccac
    21181 gcggttcatg gtaaacagcg gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt
    21241 tttgtaggtg acatagtaaa ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacaa
    21301 gatttctggc ggcgcgacac caacatcaaa gcgaaatacc agcacgctgg tcagcgggaa
    21361 gccgaagaaa aaggtgagca aaaacagcaa tgaaaagaag acattgaagt taaagcgcac
    21421 acggcgaaac tcaaaccaag tcagcgtggc aataaacagc gtgcagagca gccagaccac
    21481 aaacaggcca ctgaattgca gcagactcat gcgacctccc cggcggcaat cgccaacgcc
    21541 cgctgccagc cttgtagata gttagggcta aagaaggcaa tggtgttttt atccaccgac
    21601 gccaactggc gctgcgcttc acgcacaata tcctcgttga gatcgtcggt agtaaacagc
    21661 accggcaaat gctgttccgt catatcctgc cagaacggat tttcccggtt aagcacacaa
    21721 ggaatgcccg cctgaatcag taagcaaagc gtaccaatgc cctgctggcg ggcaaaaata
    21781 aagtaaccaa gatcgcactg acgaagtagc gccagatagg cgtcaaattc cagtttttcg
    21841 ctcaaaattt gcagattttc ttcgctgaat aactccagcc ccgcctgacg aacttcctca
    21901 atgtacgctt cgttattagg cggatatccc atcggcacca ccacttttac cgtatcgcca
    21961 aattgctgat gaacggcgcg caaggcagca atatggtcat tgctgcggtc accggagttc
    22021 cccaccagaa tggtcatttt cccttcacgt tgccgatcgt tcgccatcgt attgagagaa
    22081 gggtccatcc gcgtcgggaa gtacagcagt tcgccccgca cctttggatg cgttttggca
    22141 aaaaagctca aatcaccgcg ggtggcaaat acacagccga ctcgcttttg cgccaggcga
    22201 cgtagcgggt aaaaaagctt atatctcaag ccactggaaa gctcatacag gtctgccccc
    22261 cagatatgcc agaaaaactg gccgggctta atgccaccac tcagcagagc cagccacagt
    22321 gtgggattga actgaccgtg gaaaaaaaat cgctgctgac ggttagcttt tgctttcgcg
    22381 atgaccgctt ccgccagcga ttttttccca gggaaaaatt gcacagaaag cgccggacag
    22441 ctatcactta agccgtcgtc cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc
    22501 gcggccagcg cgtcattgaa aaaccgcaaa acggttcggt tatggtgagg gatatccgat
    22561 cccagtacgt gaatcagtac agtcatgccc gcctacgcca gagtaaaaac acgccacaac
    22621 aaagagaaaa atagacgata taagttgcca tatatgcctg cgccgcgccc agtgcaccat
    22681 gcgcagggat tagccagtgg gcaaatacca tcaataaagt gaactggctg acttccgcca
    22741 gaatataaaa ccgcagtgac gctttggcga tcaccagata accaaagaca taagcgccca
    22801 cttttaacac atcacccact aactgccagg caaagagatc gcgcatagcg gtaaatttat
    22861 tcgacaacag caaccagata gcaaaatcac gcagcaacca gacggtaaaa ctcgccgccg
    22921 ccactgccgg taagacgaat tttagcgatt taaccacttc ccgggtgata tcgcgctttt
    22981 ccgttagccg cgacaacgtg ggcagcaaat atacgctgaa cgatgccgta atgaattgca
    23041 ggtaggcatc ggaaatactg ctcacccctt gccagatccc cacttcatcc cagctatact
    23101 gcgccgccag cagtttacgc atcatgatgt aagcaacagg caaggtcacc gacgtaatca
    23161 acgccatgag cgtaaatttg ctcaactgcc ctgccagacc gttatcccag ctgggtttca
    23221 gatagcttaa cgggatgaca ccacgtttga tcaacatgat ggcggcagga attaccacca
    23281 gcgcgggaat cagcgccaga cccagcaacg ccccttcata accgcccaaa cggtaactga
    23341 cgtagtacgc gagaacgcca atcaagctgc cgacaatcag cgataacgca ttacctgcgg
    23401 catcgcgaaa gcctttcatc agcgccagta acaggttgcc ccaggcgatc cccatttgca
    23461 ccagcgccac taaacgcacc agcccctgat agtcggtatt accaaacaat ccctggctga
    23521 ttggcgcagc tgccagcaca aaaaccagcg ccatcagcgt agagaaacca agtaccatcg
    23581 ctgatgaagt gccgaccacg cggcgcagct gttgcggatt atcatggtac tgggcaacgt
    23641 atttggttac accgttaaag atgccagccc cggcaagcac gccgagcacg gtaatcaact
    23701 ggcggaaatt tgccgccagc ccaagccccg ccggaccaaa tgacaccgcc agcaacttac
    23761 cgaccagtaa cccggcacca atcttgacca gtgtactggc cgccgtccac aaggacgctt
    23821 ttgccaacga catatcagga gaagtagttc aacaaagtcg caattaccgt acgctgattg
    23881 acgggcgaca ggttgtagaa cagcggcagg cgcagcaggc gctcgctctc tttggtggtg
    23941 tagcgatctt caccgtggaa ctcaccaaag tgttcccccg cagggcaacc gtgcagcgga
    24001 atgtaatgaa acaccgccat gatttccgct tctttcagaa agttaatcaa cgcgctccgg
    24061 tcatcaatat cccgcagttt aatgtagaat atatgcgcgt tctgcacgca gccatcggga
    24121 atcgacggca gctcgatacg cccggctttc gccagaggcg ctaacgcatc gtagtagttt
    24181 tgccacagcg ccagacgttg ctggttgata cgatccgctg cttccagttg cgcccacagg
    24241 tatgcagctt gcagatcgga catcaaatag ctggagccaa tatcgcgcca ggtatattta
    24301 tcgacctgac cacggaagaa ctggctgcgg ttagtgccct tttcacggat gatctcggct
    24361 cgttcgatta acgctttatc gttaatcagc gtcgcgccgc cttcaccacc cgccgtgtag
    24421 tttttggttt catggaagct aaagcagcca atatgaccaa tggttcccag tgcacgccct
    24481 ttgtaagtgg acatcacgcc ctgagcggca tcttccacca caaacaaatt atgctttttc
    24541 gccaacgcca taatggtgtc catttcgcag gccacacccg cgtaatggac cggcacgata
    24601 acgcgcgttt tgtcggtgat cgctgcttca atcaacgttt cgtcgatgtt catggtgtcc
    24661 gggcgaacat ccacaaagac gattttcgcg ccacgcagca caaaggcatt ggcggtggag
    24721 acaaaggtgt agctcggcat gatcacttca tcgccaggct ggatatcgag cagcagcgcc
    24781 gccatctcca gcgaagcggt gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt
    24841 tgctccagcc actgctggca gcgacgggta aaaccgccat cgccacacag tttgccgcta
    24901 cccattgccg actgcatata gtcgagttcg gttcccacca ccggcggtgc gttaaatgga
    24961 atcatgtgat cacctgtata accagtacgc ggtgctttct acattcgcac cactttgtat
    25021 gtatcgttta agcgcggcgg tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc
    25081 gcgagcatac gcccagttta gcgccgtttg catcagctca gcacctgcac cgcgtccagc
    25141 cagcaggcca attcgcgcat ctgtcgcatt gagttcccgt aaagagacat agccgcgaat
    25201 atcgccggac gccgcacgta gaatcagaca ttgatgatca aaggtgccgc gcacggcatt
    25261 ttcaatccac tgtgcataaa agcgaccgct ggcgtcaggc gcataccacg gcgcacgaaa
    25321 acggctttgc gcaaatgcgg cgctagctaa ctgacgtaat gcgggaatat cggtctcttg
    25381 tgccacgaca gcaccgctat cactgacatt gttcacgggt agcgccaaat caacttcacc
    25441 ttctaccagg gagaatccca gctgttgcag ggcatccagt tcacccgtat ttgatgccgc
    25501 aattttggcc tgcacccgtg accacggcgc taacgcgtct ggcgtcagga gcggtgcttc
    25561 agacgtaatg cgcacgatgg cgctgttaac accaaagaag gcgttttccc aggttagtgg
    25621 ctcaatactg gcgcggacgg gcacgaagta actccagcag atattggccg tagccagttt
    25681 tcgctaatga actggcagca cgcttcacac cctcgtcatc gagccagccg ttacgccagg
    25741 cgatctcttc caggcaggca atcttaaagc cctggcgttt ttctactgtc tgcacaaagg
    25801 tactggcttc aatcaggctg tcgtgagtgc cagtgtccag ccaggcaaat ccgcgcccga
    25861 gcagttcaac ggtcaggttg cccgcctcga ggtacatctg gttgatggag gtaatctcca
    25921 gttcaccacg ctccgacggc ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga
    25981 aataaagccc ggtcaccgcc cagtttgact tcggctgttt tggcttttct tccagcgaga
    26041 tagcgcggaa attgtcgtca aactccacca cgccaaagcg ttccgggtcc atcacctgat
    26101 agccaaatac cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc
    26161 cctgaccgaa gaagatgtta tcgcccagca ccagacaaga aggttcaccg ttgaggaagg
    26221 tttcaccgat gataaaggcc tgcgccagac cgtccgggct gggctgttcg gcgtattcca
    26281 gctggatacc gaactcacta ccatcgccca gcaggcgctg gaaataacct ttatcttccg
    26341 gcgtagtgat gatgagaatt tcgcggatac cggccaacat cagcaccgac agcggatagt
    26401 aaatcattgg cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc gtaatcggat
    26461 gcaatcgggt gccggaaccg cccgccagga taataccttt catgtttgcc tccgctgaaa
    26521 attagccttt cagacctaaa cgctcgccct gatagctgcc gtcctgcacc tgcttccacc
    26581 agctttcatt agccagatac cactgcaccg ttttacgcat tccactttca aaggtttcct
    26641 gtggcagcca gccaagttca cgggcaattt tcgaagcatc aatggcataa cgcagatcat
    26701 gccccggacg gtcagcgaca aaggtgatca agtcacgata gtgcaccacg ccgtgcggct
    26761 tgttcggagc cagttcttcc agcagctcgc aaatggtttc cacaacatcg agattcttac
    26821 gctcgttgtg accaccaata ttataggttt caccgacttt cccggtggtc gccacgcaat
    26881 acagcgcgcg ggcgtgatct tccacataca gccagtcacg gatttgctgc ccgttgccat
    26941 ataccggcag cggtttaccc gccagcgcgt tgaggatcat cagcgggatc agtttttccg
    27001 gaaagtggta aggaccgtag ttattcgagc agttggtgat aagcgtcggc agaccgtagg
    27061 tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa taagggctgc
    27121 tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg tcaccatata
    27181 cttcgtcggt ggagatatga tgaaaacgga acgctgattt tttatcttcc gtcagcgtat
    27241 tccagtaagc ccgcgccgct tcaagcaatg tataagtccc gacaatgttg gtttcaataa
    27301 acgctgccgg gccgtcaata gaacggtcaa catggctttc ggctgccaga tgcatgacac
    27361 agtctggctg atgctcagtg aatacgcgtg ccagttctgc ccgatcgcag atatcaactt
    27421 tctcaaaggc aaagcgatcg ctttgcgcga ccggtgccag cgacatcagg tttccggcgt
    27481 aggtcagctt atcgaccact accaccgcgt cgctcgtttc gttgatgata taacgcacca
    27541 gcgccgagcc aataaacccg gcaccacctg ttatcagaat ttttctcatc agcgccagac
    27601 tcctttggca tcgacgacat actgctgatg gacattgtcg ccattgataa ctttaaactg
    27661 actatgatcg accagcatca ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc
    27721 cagagtacaa agcccggtca gttttttcgg caactggtgg atgttaggct caacaaccag
    27781 agtttcgccg ctatgccact gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg
    27841 caggtcatca atattcggtt taaacgccag accaaagcag gcgattttca gttcactggc
    27901 gcgtttatcg gtagccgcca ggcaatcagc caccgccgct ttcacctgat cgataaccca
    27961 gaacggtttg tgatcgttca cttcgcgcgc ggtacggata agccgcgcct gctgggggtt
    28021 ctgtgccacg ataaaccacg gatcaacagc aatgcagtga ccgcccacgc cagggccagg
    28081 ctgaagaata ttaacgcgag ggtgacgatt cgccaggcga atcagttccc agacgttaat
    28141 cccctgatcg gcacaaatca gcgacaattc attagcaaaa gcgatattca catcgcggaa
    28201 gctgttttcg gtgagcttac acatttccgc cgtccgcgag ttagtgacga cacactcacc
    28261 ttcgaggaaa attttgtaca gttcgctggc gcgggtcgaa caaaccggcg tcataccacc
    28321 aatcacgcga tcgtttttaa tcagctcgac cataacctgg cccggtaaca cgcgttccgg
    28381 gcagtaagca atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag tgagatccgg
    28441 acgcatctct gctaaccatt ctgccatctt ctcggttgac cccaccggcg aggtggattc
    28501 aaggatcacc agcgcgcctt ttttcagcac tggcgcaatg gagcgagcag ccgattcaac
    28561 gtaggtcata tctggctcat gatcgccttt aaacggcgtg ggtacagcaa tcagccaggc
    28621 atccgcttca actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg cagtttttac
    28681 tacgctcgcc aaatcaggct cgacgatatg gatttcgcca cgattgatgg tatcaaccgc
    28741 gtgttggttg atatcgacac caatcacctg tttttgccgt gaggcaaacg ctgctgctgt
    28801 aggcagcccg atgtaaccca gtccgataac agaaatggtc gcaaaactca tagtgatatc
    28861 cgattatttt ttaacgcttc cagaatgcga gagcatgcct gaccatcacc atacgggtta
    28921 tgggcgcggc tcatagtttg atattcgttt tcgtctttta aaagacgcgt cacttcctcg
    28981 acaattcgct gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga
    29041 cgctcagtgg tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc ttcttcctga
    29101 atgccgcctg agtcggtcaa aatcagccag gcgtggttca tcaaccagac aaacggtaaa
    29161 tactcctggg gatcgatcag aatgacattt ttcacatgcc ccagaatgcg attgaccggc
    29221 tctctgacgt tcgggttgag atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg
    29281 gcaatgtctg ccaacgcgtg gcagatttct tcaaaaccac gaccgaaact ctcacgcctg
    29341 tgaccggtca ccagaatcat ctttttatcg gggtcgataa acgggtaatt tgccgccagt
    29401 tctgaacgca gcgtgtcgct gctcatcacc tgatcacgca cccataacag tgcatcaatg
    29461 actgtattac cagtaatgaa gattcggcta tccgcaacgt tttcacgcag caagttttgc
    29521 cgggaagttt cggttggaga gaagtgatac atcgccagat gcccggtcaa tgtacggtta
    29581 gcctcttccg gccacggcga atagagatcg cccgtgcgta aaccagcctc aacgtgacca
    29641 acaggaatac gctgataaaa cgccgccagg ctggttgcca gcgtcgttgt tgtgtcgccg
    29701 tgaaccagca cgacgtctgg tttgaactcg gcaagaatag gttttagccc ttccagaatc
    29761 cgacaggtta tctctgtcag gccctgtcct ggctgcatta tgttgagatc gtagtcaggt
    29821 acaatggaaa agagtttcag cacctgatcg agcatctccc gatgctgcgc agtgacgcaa
    29881 actttagcct caaaaaaagg agcttttgcc aacgcatgca ccaacggagc catcttgatg
    29941 gcttccgggc gcgtaccaaa tacagtcagt actttcacat cgattctctt cgaataagcg
    30001 gcgagcgcct ttgcgctcgc cgcagcagtg ttgctatttc gagcaacggc gggttaatgc
    30061 gacaccagcc ccgatcagcc ccccgacaat gccccacata atcatcagga aggcacgacg
    30121 tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg
    30181 cggatccagg gttggaccaa cattcagggt gtttaacatg gcccgattct gatcatagtc
    30241 gagatcaaag gccggaccga cggcctgtaa attttccagt cgagcctgga gcattggacg
    30301 cccaagcagg aacatttctg aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg
    30361 cgaaatatta tgctgctcag caattttcag cgcctgctca atgctgttca tccggcggtc
    30421 gtagatggct ttcgccacct cttcctgacg cttcacctga gctttcatct ggatggtgcg
    30481 tgccgcccat gcgcctttca gctcatcatt cagatggctg gctgcacgct ggctggcaaa
    30541 agcaacatac tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa taagcttcac
    30601 gctgtcattg accgcgcggg taaagtctcc ggggataaac tggatgttgt taatcatttc
    30661 atccagcaac gccgcatcgg ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt
    30721 ttgcagccag aactctctgc gggtatccca cgaggccagt tgcataacaa actctttata
    30781 ggcttcgtcc atgaccgatg gttgatcggc agaagccatg tttgaacgga catccaggtt
    30841 acgcaaaaat tgctgctgcg agtaatatcc ccccagcata ttcaccgttg gacgatcggt
    30901 aatcgccgtc gagctccact cctgacgagc aaaaaaagta tacgccagcg cgattaacgc
    30961 aaacgccagc cccatgccaa taatccatag cttcccagcc cacaaggtac gaaacaaccc
    31021 acgaatatcc agttcatttt cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat
    31081 cacatcctca tttatttggt taaattgggg ctgccaccac gatttctacg cagtctgcgt
    31141 tttacgcgct taataaagcg agcaactttc caggcacgct taatgcaata tccatagagg
    31201 aagaatgcta gcaaaaagag caccagcatg acccactccg ggacaaaatg agaatattct
    31261 gccagcacgc caatggaagc gagcagtgct gcggcaaggg taatcagcac aaacgcctga
    31321 cgggaagtaa acccggcacg catgatcaaa tggtgaatat gctgacggtc aggagagaat
    31381 gggctcatgc ctttacgcag gcgacggtac ataatcgcca ccatatccat tagtggaatg
    31441 gcgattatcc acaaagcggt aaccgggctg atgggatggg ttttgccctg ggtcgtttcg
    31501 agcaggatcc agataacggt aaaaccaatc agcgtactgc ccgcatcacc cataaagact
    31561 ttgtagcggc gacccaggat accaaggtta agcatgatgt atggcaggat ggcggcgatc
    31621 atcgcaaagc accagattgc gaggctggtt tgcccgtcga accacaaaat cataccgatt
    31681 gctgcaaacg agacgcagga caacccgccc agcaagccat caatgccatc aaccatgttg
    31741 aacgcattaa tggccgccca gacggcaaat agcgtcagga agtaaccaaa cggtccgagc
    31801 accatctccc aggagccaaa gatataaccc aggctactga gataaagttt gccgaacacc
    31861 atcataacaa tgccaacagc ggcctgtatg gtggcacgga tttttacgct gatatcaaaa
    31921 cggtcatcca gcgcgccaat gaaaacaagc acaccggcac aagcgagata gagagatgca
    31981 tgcggaatat agtaatcgac aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc
    32041 cccccaacga gaggtatcaa tccctggtga cgtttgcgga agtttggttt atccactaaa
    32101 ccgacttttt ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata
    32161 ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc atgctctcag agaaaattat
    32221 tagcagaagt ataaccacga agacctttat tcagaaggga aagtccgttc cgaacccacc
    32281 agcactgtgc aaaaattaac catttgatat gcaaattgca taacatgcca gcaaattcca
    32341 ggaattttcc taacatcgcc agcgcggcat taagattaca gatataaaag caaaacgcca
    32401 cgtaaacacg tggcgttttt ggcataagac aaatttatga gcgtttcatc atttcgaaga
    32461 aatcgtcatt ggtcttggtc atggccagtt tattaatgag gaattccatt gcatcgattt
    32521 cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt ctgcagttct tcctgagtcg
    32581 tgagcagctc ttctttacgg gtaccggaac ggttgtagtc gatagccggg aagacgcgtt
    32641 tttcagcgat cttacgagag aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt
    32701 aaataacttc gtccatttta gaaccggtat cgataagcgc cgtcgcgata atggtcaggc
    32761 tgccgccctc ttccacgtta cgcgccgcac cgaagaagcg tttcggacga tgaagggcat
    32821 tggcgtccac accaccggtc aacactttac ctgacgccgg aacaacagtg ttgtaagcgc
    32881 gcgccagacg agtgatggag tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc
    32941 gtttggcctt ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa
    33001 aggtagaagc aacaacttca ccttttacca gacgctgcat ctcggttact tcttccggac
    33061 gttcgtcgat cagcagaacc atcagcacac aatccgggtg gttgtaagca atgctctgag
    33121 caatgttctg cagcagcata gttttaccgg ctttcggcgg tgccacaatc agaccacgct
    33181 gaccacgacc gataggtgat gccagatcca gtacgcgagc ggttaaatct tccgtagaac
    33241 cgttaccacg ttccatacgc agacgagagt ttgcgtgcag cggggttaag ttctcaaaga
    33301 ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg
    33361 caaaatagcg ttcaccttct ttcggcgggc gaatcttacc agagatggta tcaccagtgc
    33421 ggaggttgaa acggcggatt tggctagggg aaacgtagat gtcatcagga ccggcgaggt
    33481 aggagctgtc tgcggaacgg aggaaaccaa atccatcctg caatatctcc agtacgccat
    33541 caccaaagat atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct
    33601 gcttacgcat acgagccagg ttttccagcc ccatattttc gccgagagtg atcagctcag
    33661 aaaccggcgt attctttaat tcggtaagat tcatagtggt gtgagttctt aaacttgggg
    33721 taaatctcga acttaatgtt gtgaatggta tggcaggatc atccatgcct gtttacggtc
    33781 atcgactcat gtcttttcgc tgcctggtca caggagagta cgcagaactg aaacgacaag
    33841 acggattgag tgacgagcca gaaatctgtt cacttcgcat ttaagataaa acgggaagcg
    33901 ttggaaatta caagattcaa acttaataag gtatgtttaa tacgaagtca acactaagtt
    33961 agcatgactc acgacgggcg tccagttttt agcgacgggg cacccgaaca tgaaattccc
    34021 ttacgccagg ttagcgtcga ggaactcttt caactgacct ttagacagtg cacccacttt
    34081 ggttgccgcc acttcaccgt ttttgaacag cagcagagtc gggataccac ggatgccata
    34141 tttcggcgca gtgccagggt tttgatcgat gttcagtttt gcaacggtca gtttgccctg
    34201 atattcgtca gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc
    34261 tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt acatccgtgt caaaactgtc
    34321 gtcagtcagg tgaataattt tatcgctcat atataactcc acaggaataa gcctggcgtg
    34381 ttggtttcgt tgttggtgta acattaacca actaaaggtt gactttattt caccggatac
    34441 gctttcgtaa agcaatagta agctgatatt ctaccacact atgagcaaaa cacatttaac
    34501 agaacagaag ttttccgact tcgccctgca tccgaaggtt gtagaagtcc ttgaaaaaaa
    34561 agggtttcat aactgtacgc ccattcaggc actggccctt ccgctgacgc tggcgggtcg
    34621 tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac
    34681 gtttcattat cttctctctc atcctgcgat tgccgatcgc aaggtgaatc aaccacgtgc
    34741 cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc catgccgacg cagaaccgct
    34801 ggcggaagct accggcctga agctgggtct ggcttacggt ggtgatggct acgacaaaca
    34861 gctgaaagtg ctggaaagcg gcgttgacat tctgattggc actacggggc gtttaattga
    34921 ctacgccaag cagaaccaca ttaacctcgg tgccattcag gtggtggtac tggacgaagc
    34981 cgatcgcatg tacgatctgg gctttattaa agatatccgc tggctgttcc gccgtatgcc
    35041 gcctgcaaac cagcgcctca acatgctgtt ctccgccacg ctttcgtacc gggtacgtga
    35101 actggcgttc gagcagatga acaatgccga atatattgaa gtggaaccgg aacagaaaac
    35161 gggccaccgt attaaagaag agcttttcta cccttctaac gaagaaaaaa tgcgtttgct
    35221 gcaaacgctg atcgaagaag agtggccaga cagagcgatt attttcgcca ataccaaaca
    35281 ccgttgtgaa gagatctggg gccacctggc ggcagatggt catcgtgtcg gtttgttgac
    35341 aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc gtggcgatct
    35401 ggatattctg gttgccaccg acgttgccgc gcgtggtttg catattccgg cagtgacgca
    35461 cgtctttaac tacgatttac ccgatgactg tgaagattac gttcaccgta ttggtcgtac
    35521 aggtcgcgca ggcgcaagcg gtcactctat cagcctggcg tgtgaagagt atgcattgaa
    35581 tttgcctgct attgagacct atattggtca ctcaattccg gtaagcaaat acaatccgga
    35641 cgcattgatg accgatctgc caaaaccgct gcgcctcacg cgcccacgta caggcaatgg
    35701 tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac
    35761 tcaaaatcat tcaaactgta tcaagatggc aagagaatga atcccgatga gcatactgta
    35821 gtaagtgatt caggtgaaag aatgcagcca acacagagac agattgaagg atgaagagta
    35881 tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc
    35941 tggttgtgcg cgaggtggct ggaagcatcc agacgctgac gcgaataaaa cgcaaagtgc
    36001 gtctggctgc tggcctgaat agcgaaaatg ccctgtctaa tgaagcaatg gagcgcggtt
    36061 ggcaatgtct gcgcctgttt gctgaacgtc tgcaagatat ccctccctcg caaattcgcg
    36121 ttgtcgctac ggcgacatta cgtcttgccg tcaatgcggg tgattttatt gccaaagcgc
    36181 aggaaatcct cggttgtccg gtacaggtga tcagcggtga agaggaagca cgtctgattt
    36241 atcagggcgt tgctcacaca actggcggtg ccgatcagcg cctggtggtg gatataggcg
    36301 gtgccagtac tgaactggta accggcacgg gtgcacaaac cacctcgttg ttcagcctgt
    36361 cgatgggctg cgtcacctgg ctggaacgct attttgccga tcgtaatctg gggcaggaaa
    36421 attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc gatgaattac
    36481 ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac cgtgcaggca ttacaggaaa
    36541 tcatgatggc acaggggatg gatgaacgca ttaccctgga aaagttgcag caattgaaac
    36601 agcgagccat tcattgcggt cggctggagg aactggagat tgacgggctg acgctggaac
    36661 gtgcgttagt gttcccgagt ggtctggcga tcctgatcgc catttttacc gaactgaata
    36721 ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc
    36781 atctggccgt agaccaggat attcgcagcc gtacgctgcg taatattcag cgccgcttta
    36841 tgatcgatat tgatcaggcg cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg
    36901 tggaaaagga atggcatctt gaagcaataa gccgcgattt gctcatcagc gcctgtcagc
    36961 ttcatgaaat cggcctgagc gttgacttta aacaagcgcc gcaacacgct gcttatctgg
    37021 tgcgtaatct ggatcttccc ggttttaccc ccgcacagaa aaaattgctg gcgacgctac
    37081 tgctcaacca gactaatccg gtcgatctct catcgctgca tcagcaaaat gccgtaccgc
    37141 cgcgcgtcgc agaacaactc tgccgtttac tccgcctggc catcattttt gccagccgtc
    37201 gccgtgacga tctcgtgcca gagatgacat tacaggctaa ccatgaactg ttgaccttga
    37261 cgcttccgca aggttggcta acccaacatc cgctgggtaa agagattatt gctcaggaaa
    37321 gccagtggca aagctatgtc cactggccgc tggaagtgca ttaatgagta agtgccggat
    37381 gcgatgctga cgcatctttt ccggccttga ttatttccct cgttttgccg ccatcatcgc
    37441 tttcagattc gccagatggc tttgcccttt ctgcatccgt tcttcggcgc tgaccacttt
    37501 gcgctcctgt tcccaaatca gatcatcctg cggcagctcc agcaaaaagc ggctcggctc
    37561 cgggcgcacc agctcgccgt actgacggcg ttctttacac agcgtaaagg tcaattcctt
    37621 ctgggcgcgg gtaatgccga cataggccag ccgccgctcc tcatcgatat tatcttcatc
    37681 gatgctgctc tggtgcggca aaaacccttc ttccataccg accatgtaga cataaggaaa
    37741 ctccagccct tttgacgcgt gcagagtcat cagttgcacc tgatccagct cttcttcact
    37801 ctcaccacgc tccatcatgt cgcgcaaagt aaagcgcgtc accacctggg tgagcgtcat
    37861 cggctcatcc agttcgctgc cttccagcat ctccgtcatc cagctaaaca gttggttgac
    37921 gttcttcatg cgcatttcgg cggctttcgg gctgggcgat gtttcgtaca gccaggattc
    37981 ataatccatg ccgtggatca gatcgcgcac cgcggcaatc ggctcccgct ccgccagacg
    38041 ctggatttct gccaaccagt gagtaaagcg ggtcaatgct tcataaccac gtccgctaag
    38101 cgtctgactc aggcccatat caaagctggc ggtaaacatg cttttattgc gcgtcatcgc
    38161 ccactcaccc agctttttca gcgtcgccgg gccaatctct ctcttcggcg tgttaacgat
    38221 acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg cgcagataag ccagcaagtc
    38281 cttgatttca ggacgagaga aaaacgacgt accaccagaa attttgtacg ggatgcggtt
    38341 ttgcatcagg aatttttcaa acactcgcga ctgatggtta ccgcgataaa gaatagcgta
    38401 atatttgtac tgcgttttat tgacgaagtg atgcgcaatc agttcaccag tgacgcgctc
    38461 ggcttcatgt tcttcgttat tcgcgcttaa aactttaagc tctgtgccgt aacccagttc
    38521 ggagaacaga cgcttctcaa agacgtgcgg gttattggcg atcaggatgt tcgccgcttt
    38581 caaaatacgt ccggaagagc ggtagttctg ctcaagctta atcaccttca gcgccggaaa
    38641 atcctgactc agcagcacca ggttttgtgg acgtgcgccg cgccaggagt agatcgactg
    38701 gtcgtcatcg cccaccacgg taaagcgcgc gcggctgccc accagcagtt tcaccagttc
    38761 atactggctg gtgttggtgt cctgatactc atccaccagc agatagcgaa ttttgttctg
    38821 ccagcgctcg cggacttctt cattgcgttg cagcagcaac gtcggcaata aaatcagatc
    38881 atcgaagtcg agaacattgc acgctttcag gtgtgcatca tacagcccat aacaatgggc
    38941 aaaaatacgg tcccgctcgc caatcgcact ggctgccgcc tgggacggtg ttttgagatc
    39001 gttcttccag ttagagatgg tcgaaatcaa ctgttgcagg agaactttgt catcttcaat
    39061 cagcccttcg gtcaactctt tgagcaacgc cagctgatcg gtatcgtcaa acagcgaaaa
    39121 gttcgccttc atccccagcg ccgcatactc acgcttgatg atatccagcc ccagcgtgtg
    39181 gaaggttgag atcatcagcc cacgcgcctc tttgcgaccc agcgtctgtc cgacacgctc
    39241 tttcatctcg cgcgccgcct tattggtaaa ggtcaccgcc gcgatatgtc gcgcctgata
    39301 accgcaaccg cggatcagat gggcgatttt attggtgatc acacgagttt taccggaacc
    39361 cgcgcccgcc agcaccaggc aggggccggt aacgaattcg acagcttgtt gttggccggg
    39421 gtttagacgc ataggtgtat tgctcaatct tcgaacgggg ggaggattgt agcagaatcg
    39481 catttgaatg tgaccgagtg taagccgcta accggttggc tgcggatgtt taaatcagag
    39541 agggaagacc gaaaccggag ttcatcgtga atccattaga cttagaaaat atcgggtcgt
    39601 aacgttgtgt tattactttt tcagagttta ctaaggagag gtgtactcgt ggcgattaat
    39661 ttcagtccta aggttggtga aatactggaa tgcaactttg gtaactatcc tgttagccag
    39721 aatggacagt tcagcaccac gtattacgat ggccgcattc ctcctgaaat gataaaaaac
    39781 aggtta
//
LOCUS       DAJTNA010000014        39588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTNA010000014 DAJTNA010000000
VERSION     DAJTNA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39588
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.014"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..349)
                     /locus_tag="OO161_000626"
     CDS             complement(<1..349)
                     /locus_tag="OO161_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
                     /protein_id="HCR5461799.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYL"
     gene            complement(808..894)
                     /locus_tag="OO161_000627"
     tRNA            complement(808..894)
                     /locus_tag="OO161_000627"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(858..860),aa:Leu,seq:gag)
     gene            complement(909..1241)
                     /gene="secG"
                     /locus_tag="OO161_000628"
     CDS             complement(909..1241)
                     /gene="secG"
                     /locus_tag="OO161_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5461800.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1469..2806)
                     /gene="glmM"
                     /locus_tag="OO161_000629"
     CDS             complement(1469..2806)
                     /gene="glmM"
                     /locus_tag="OO161_000629"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5461801.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2799..3647)
                     /gene="folP"
                     /locus_tag="OO161_000630"
     CDS             complement(2799..3647)
                     /gene="folP"
                     /locus_tag="OO161_000630"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5461802.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3737..5671)
                     /gene="ftsH"
                     /locus_tag="OO161_000631"
     CDS             complement(3737..5671)
                     /gene="ftsH"
                     /locus_tag="OO161_000631"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5461803.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPSFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGTSNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5771..6400)
                     /gene="rlmE"
                     /locus_tag="OO161_000632"
     CDS             complement(5771..6400)
                     /gene="rlmE"
                     /locus_tag="OO161_000632"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5461804.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6526..6819
                     /gene="yhbY"
                     /locus_tag="OO161_000633"
     CDS             6526..6819
                     /gene="yhbY"
                     /locus_tag="OO161_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5461805.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6976..7452)
                     /gene="greA"
                     /locus_tag="OO161_000634"
     CDS             complement(6976..7452)
                     /gene="greA"
                     /locus_tag="OO161_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5461806.1"
                     /translation="MQTIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7700..9133
                     /gene="dacB"
                     /locus_tag="OO161_000635"
     CDS             7700..9133
                     /gene="dacB"
                     /locus_tag="OO161_000635"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5461807.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9173..10345)
                     /gene="cgtA"
                     /locus_tag="OO161_000636"
     CDS             complement(9173..10345)
                     /gene="cgtA"
                     /locus_tag="OO161_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5461808.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWIEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(10361..11326)
                     /locus_tag="OO161_000637"
     CDS             complement(10361..11326)
                     /locus_tag="OO161_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5461809.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSASLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYSLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11453..11710)
                     /gene="rpmA"
                     /locus_tag="OO161_000638"
     CDS             complement(11453..11710)
                     /gene="rpmA"
                     /locus_tag="OO161_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5461810.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNHKFISIEAE"
     gene            complement(11731..12042)
                     /gene="rplU"
                     /locus_tag="OO161_000639"
     CDS             complement(11731..12042)
                     /gene="rplU"
                     /locus_tag="OO161_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5461811.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12301..13272
                     /gene="ispB"
                     /locus_tag="OO161_000640"
     CDS             12301..13272
                     /gene="ispB"
                     /locus_tag="OO161_000640"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5461812.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13498..13776
                     /gene="sfsB"
                     /locus_tag="OO161_000641"
     CDS             13498..13776
                     /gene="sfsB"
                     /locus_tag="OO161_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5461813.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13824..15083)
                     /gene="murA"
                     /locus_tag="OO161_000642"
     CDS             complement(13824..15083)
                     /gene="murA"
                     /locus_tag="OO161_000642"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5461814.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(15138..15392)
                     /gene="ibaG"
                     /locus_tag="OO161_000643"
     CDS             complement(15138..15392)
                     /gene="ibaG"
                     /locus_tag="OO161_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5461815.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15552..15845)
                     /gene="mlaB"
                     /locus_tag="OO161_000644"
     CDS             complement(15552..15845)
                     /gene="mlaB"
                     /locus_tag="OO161_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5461816.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15845..16480)
                     /gene="mlaC"
                     /locus_tag="OO161_000645"
     CDS             complement(15845..16480)
                     /gene="mlaC"
                     /locus_tag="OO161_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5461817.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16499..17050)
                     /gene="mlaD"
                     /locus_tag="OO161_000646"
     CDS             complement(16499..17050)
                     /gene="mlaD"
                     /locus_tag="OO161_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5461818.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17055..17837)
                     /gene="mlaE"
                     /locus_tag="OO161_000647"
     CDS             complement(17055..17837)
                     /gene="mlaE"
                     /locus_tag="OO161_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5461819.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17845..18654)
                     /gene="mlaF"
                     /locus_tag="OO161_000648"
     CDS             complement(17845..18654)
                     /gene="mlaF"
                     /locus_tag="OO161_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5461820.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18864..19841
                     /locus_tag="OO161_000649"
     CDS             18864..19841
                     /locus_tag="OO161_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5461821.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKFARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19855..20841
                     /gene="kdsD"
                     /locus_tag="OO161_000650"
     CDS             19855..20841
                     /gene="kdsD"
                     /locus_tag="OO161_000650"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5461822.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSTTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20862..21428
                     /gene="kdsC"
                     /locus_tag="OO161_000651"
     CDS             20862..21428
                     /gene="kdsC"
                     /locus_tag="OO161_000651"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5461823.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21425..22000
                     /gene="lptC"
                     /locus_tag="OO161_000652"
     CDS             21425..22000
                     /gene="lptC"
                     /locus_tag="OO161_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5461824.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21969..22526
                     /gene="lptA"
                     /locus_tag="OO161_000653"
     CDS             21969..22526
                     /gene="lptA"
                     /locus_tag="OO161_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5461825.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22533..23258
                     /gene="lptB"
                     /locus_tag="OO161_000654"
     CDS             22533..23258
                     /gene="lptB"
                     /locus_tag="OO161_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5461826.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRHLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23306..24739
                     /gene="rpoN"
                     /locus_tag="OO161_000655"
     CDS             23306..24739
                     /gene="rpoN"
                     /locus_tag="OO161_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5461827.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24762..25049
                     /gene="hpf"
                     /locus_tag="OO161_000656"
     CDS             24762..25049
                     /gene="hpf"
                     /locus_tag="OO161_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5461828.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25167..25658
                     /gene="ptsN"
                     /locus_tag="OO161_000657"
     CDS             25167..25658
                     /gene="ptsN"
                     /locus_tag="OO161_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5461829.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25704..26558
                     /gene="rapZ"
                     /locus_tag="OO161_000658"
     CDS             25704..26558
                     /gene="rapZ"
                     /locus_tag="OO161_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5461830.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26555..26827
                     /gene="npr"
                     /locus_tag="OO161_000659"
     CDS             26555..26827
                     /gene="npr"
                     /locus_tag="OO161_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5461831.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27041..27673
                     /gene="yrbL"
                     /locus_tag="OO161_000660"
     CDS             27041..27673
                     /gene="yrbL"
                     /locus_tag="OO161_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5461832.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27670..28398)
                     /gene="mtgA"
                     /locus_tag="OO161_000661"
     CDS             complement(27670..28398)
                     /gene="mtgA"
                     /locus_tag="OO161_000661"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR5461833.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(28395..29048)
                     /gene="elbB"
                     /locus_tag="OO161_000662"
     CDS             complement(28395..29048)
                     /gene="elbB"
                     /locus_tag="OO161_000662"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5461834.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADATELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29278..31614)
                     /gene="arcB"
                     /locus_tag="OO161_000663"
     CDS             complement(29278..31614)
                     /gene="arcB"
                     /locus_tag="OO161_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5461835.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDILMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31710..32639)
                     /locus_tag="OO161_000664"
     CDS             complement(31710..32639)
                     /locus_tag="OO161_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5461836.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLEHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            33313..37773
                     /gene="gltB"
                     /locus_tag="OO161_000665"
     CDS             33313..37773
                     /gene="gltB"
                     /locus_tag="OO161_000665"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5461837.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRTGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37786..39204
                     /gene="gltD"
                     /locus_tag="OO161_000666"
     CDS             37786..39204
                     /gene="gltD"
                     /locus_tag="OO161_000666"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5461838.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            39388..>39588
                     /locus_tag="OO161_000667"
     CDS             39388..>39588
                     /locus_tag="OO161_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461839.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLI"
BASE COUNT         9774 a        10735 c         9806 g         9273 t
ORIGIN      
        1 atagataagg gatatacatc cccagcattt tgacaacttc atccggatca ctctggagca
       61 cactaatcgc aaatccatca ttgaaagttg taccaaaggt acaccatgaa tagaggctaa
      121 tactcatgtc tgccgcagtc agtaacacaa atggtgcggc aacaagcagg cgaagcggaa
      181 gggatgatat acgttttaca accaggatgg tcaggacgaa gaacagcaat cgctgaacca
      241 tatgcgcgcc ggaagaggcg actaagttcg taataccaaa aacaataaca caaagataca
      301 acaaccaatg agatttaaaa cttctagcaa atttgttgaa tactgtcatc cagaaatcca
      361 agttcacaac caatataaac aattatcaac aatgttcaat tttatatcag acatgttttt
      421 caactctgat ccatatcaga ccgaaacact gttagttttt tctttttaaa tgatgcggcc
      481 attgcgttct cagtaattaa ataacattat caatgcgtct ctaaacatta cgcagaacgt
      541 agccatcagt agttatactg acaggagcag gatggtttat cgagaagtaa acatatccgt
      601 attcgtaccg gaactggagt tgacacaata aagtgccaat tatgtcagca gaagggaaaa
      661 gtttataact aaggcgtacc ggcaccatcg tttcaaggta ccagctacga gtaaagcaac
      721 tggacgagat acagatacct gacaaccatt cctcagacca ggaccaaaac gaaaaaagac
      781 gcttttcagc gtctcttttc tggaatttgg taccgaggac gggacttgaa cccgtaagcc
      841 ctattgggca ctaccacctc aaggtagcgt gtctaccaat tccaccacct cggcacggat
      901 actacttttt agttcgggat atcgctggtc ggcttagccg gagcagctgg ttgagtttgt
      961 tcggttttcg ccggtgcact cagattttcc cattcgctac ctttattggt tttgttgcta
     1021 ttgatgttac ccagcaccag actgatgatg aagaataacg ttgccagcag cgccgtcatg
     1081 cgggtcatga agttaccaga accacttgaa ccaaacagcg tagcggaagc gcctgctccg
     1141 aaggaggctc ccatatcagc gcctttacct tgctgcagca tgatcagacc aacaaggcca
     1201 attgccacaa taaggaaaac taccaaaaga gcttcgtaca taatcaacct gttccttgcg
     1261 gagttgccgc gtaccaatgc ttcaaccaat aaagcgggag tttttaatct ttcccactga
     1321 agcgggtgtg aatactaacc aaagcgaatg accttcgcaa gggcaatttt atcgcattgt
     1381 atcaactgcg gaaaaaagca gcaaaaccga tgtgttcgct gagaaaaaag gcgacaatcg
     1441 ccgccttttt agccagtcat ttatcgtttt aaacggcttt tactgcatcg gcgatgcggt
     1501 gtgcaaattc agtcacctgc gcttcgtctt cgccttccac catcacgcga attaacggtt
     1561 cggtgccgga tttacgcagc aacacgcgtc cacggttgcc cagcgcagct tcaacctctg
     1621 cggtcacggc tttaactgac tcatgctcaa gcggatcgcc gctacctgcg gtgtaacgca
     1681 cgttaaccag aatctgcggg aacattttca tgccgctgca aaggtcgtgc aggctcatat
     1741 ggttacgtgc catcgccgcc agcacctgca agccagcaac gatgccgtca ccggtagtgg
     1801 ttttatccag caggatcaca tgaccggaat tctctgcacc gatacgccag cctttctcct
     1861 gcattttttc cagtacgtag cggtcaccca ctttcgcgcg cgcaaatgga atacctaact
     1921 gtttcagcgc cagctcaagc cccatgttac tcatcaatgt acccacagca ccaccacgca
     1981 gctggccctg acgaagacct tcacgcgcga tgatatacat gatctgatca ccatcgactt
     2041 tattgccttc atggtcaacc ataatcacgc gatcgccgtc gccgtcgaag gcaataccga
     2101 gatccgcttt ttcagccagc acacgagcct ggagcgcgcg aacgtcggta gcccccactt
     2161 cggcattgat gtttacaccg ttcggctcac aaccgatagc gataacgttc gcccccagtt
     2221 cacgcagcac gttcggcgcg atgtgataag tcgcaccgtt tgcgcaatcc accacaatct
     2281 tcagttcgct gaggctaagt tcgttcggga acgtggcttt gcaaaactcg atatagcgac
     2341 ccgcagcatc aacgatacgg ctggctttac ccagttctgc cgaatcaaca cagctgatct
     2401 ctttttccat ttccgcttcg atggcctctt ctaccgcatc cggtagtttg gtgccgtcga
     2461 tagagaaaaa tttaatgcca ttatcgtaga acgggttatg cgatgcagat atcacaattc
     2521 cggcctctgc gcggaaggta cgcgtcagat aagccacggc cggtgttggc atcgggccag
     2581 tgaagagtgc ggaaaggccc gctgccgcca gacccgcttc cagtgctgac tccagcatat
     2641 agccagaaat acgcgtgtct ttaccaataa tgatcttacg ggagccgtgg cgcgccagca
     2701 ctttacccgc ggcccaaccc agcttaagca caaaatcagg tgtgatcggc gcatccccta
     2761 cacgaccacg aatcccatcg gtaccgaaat atttacgatt actcatagcg tttgttttcc
     2821 tttgcagaca gagtggcttc caccacccgc atcgcttcta cggtttcttt gacgtcatga
     2881 acacgaataa tgtgcgcgcc ttgcattgcg gcaatgaccg cacaggccag actaccgctc
     2941 aggcgctcgg acggccccac gttcagcagc tgcccaatca tcgattttcg tgacatacct
     3001 accaacagcg gcaggttgaa atggtgaaat tcagccaggc gcgccagtaa tgaatagtta
     3061 tgggagagat ttttaccgaa accgaatccg gggtcgagca acaatttctc ttttgcgata
     3121 cccgcctgct cgcaacgtgc tatttgctca ataaagtagc gattcacttc tgcaaagaca
     3181 tcgtcatact tcggagcttc ctgcatggtt tttggatttc cctgcatatg catcagacaa
     3241 accggtaaac cggtttctgc cgccgcctcc agagcgccag gttcggaaag ggagcggata
     3301 tcgttaataa tgtgagcgcc aactttcgct gactcacgga tgacttctgg tttggatgta
     3361 tctaccgaga gccagacttc gaagcgttga gcaattgcct caaccacagg aataacacgt
     3421 tgcaactctt cttcaacgct aacttccgcc gcccctgggc gcgtggactc gccaccaacg
     3481 tcaatgatcg tcgcgccagc gttgatcatc agattcgcat gtttcaccgc atctatcagc
     3541 gagttatgcg tgccaccatc cgaaaaggaa tcaggcgtga cgttgaggat ccccattacg
     3601 tgaggatggc taaggtccag tgaagtgcct tgggcaaaga gtttcatggt gttatccctg
     3661 ctatgaattg ataagaaaaa accccggagc acgccccggg gttttcggta caaatacagt
     3721 catctgatgc gggaacttac ttgtcgccta actgctctga catggtgtta cccgggttcg
     3781 gcgtacgcgg ttcatcaacc ggacgaggag cctttggact accattgtcg ccagaattgt
     3841 tagaagtgcc tggttcttcc cagcccgctg gcggacgtac atcgcgacgt gccatcaggt
     3901 catcaatctg cggtgcgtcg atagtctcat atttcatgag agcatctttc atcgcatgca
     3961 gaatatccat attgtcggtc agaagctgac gcgcacgatt atagttacgc tcaatcagtg
     4021 ctttcacttc ctggtcgatg atacgtgcag tttcatcgga catatgtttc gctttcgcta
     4081 cgctacggcc gaggaacact tcaccttctt cttccgcgta cagcagtgga cccaatttct
     4141 cagagaagcc ccactgagtc accatgttac gcgccaggtt ggtcgcaact ttaatatcgt
     4201 tggacgcacc ggtagaaaca tgttccggcc cgtagatgat ctcttctgcc agacgaccac
     4261 cgtacagcgt agaaatctgg ctttccagtt tctgacggct ggcgctgatt gcgtcgccct
     4321 caggcaagaa gaaagtcaca cccagcgcac gaccgcgtgg gataatcgtc actttgtgca
     4381 ccggatcgtg ttccggcacc aggcgaccga taatcgcatg acccgcttcg tggtaagccg
     4441 tcgattcttt ctgcgcttcc gtcatcacca tggagcgacg ttccgcaccc atcatgattt
     4501 tatctttcgc tttttcgaac tcaatcatcg acacaacgcg tttgttgcca cgagcagcga
     4561 acagtgccgc ttcgtttacc aggttcgcca ggtcagcacc ggagaaacta ggagtaccac
     4621 gggcaatgat tgccgcgtcg atatcgggtg ccaatggtac gcgacgcatg tgaactttca
     4681 ggatctgctc acgaccgcga acatctggca agccaaccac aacctgacgg tcgaaacggc
     4741 caggacgcag cagggccggg tcaagaacgt ccggacggtt agtcgcggcg ataacgatga
     4801 taccttcgtt accttcgaag ccatccatct caaccagcat ctggttcaga gtctgttcac
     4861 gttcatcgtg accaccgccc agaccagcgc cacgctggcg gcctacggcg tcgatttcat
     4921 cgataaagat gatgcacggt gccgctttct tcgcctgttc gaacatgtca cgaacacggg
     4981 atgcacccac accgacgaac atttctacga agtcagaacc ggagatagta aagaacggaa
     5041 ctttcgcttc gcctgcaatc gctttcgcca gcaacgtttt accggtaccc ggaggaccga
     5101 ccatcaggac gcctttcggg atcttaccgc cgagtttctg gaagcggctc ggctcgcgca
     5161 gatactctac cagttcagca acttcttctt ttgcttcgtc gcagcccgca acgtcagcaa
     5221 aggtcgtttt gatctgatct tccgtcagca tgcgcgcttt gctcttacca aacgacatgg
     5281 cacctttgcc accgccgccc tgcatttgac gcatgaagaa gatccagaca ccaatcagca
     5341 acagcatcgg gaaccaagag atgaagatag aagccagcag gcttggttct tcaggcggtt
     5401 caccgacaac cttgacgttc ttggtcaaca ggttatccag taatttcgga tcctgaaccg
     5461 gaatgtaagt ggtataacgg ttactatctt tcttggtaac gttgatttca cgtccgttga
     5521 tacgcgcttc acgaacctgg tcgttattga cctcttgtag gaaggtagag taatccacct
     5581 tacggccatt agactcgctg ggcccaaagc tctggaatac tgacatcagc acaacggcaa
     5641 tgaccagcca gagtattagg tttttcgcca tgtcactcaa gggattaacc tcttattaca
     5701 actgtgttaa aaacagcgtc aggatactct atatccagca tctttcaaac tttcgtctga
     5761 aatctcccgg ttagggttta cgcccggtcg ctacaatata cacttcccgc gaacgtgcac
     5821 gagaagagtc cggcttacga actttgacct tcgtaaacag ggagcgaatt tcccttagat
     5881 actcatcgaa accttcgccc tggaacacct tcactacaaa actgccacct ggcgctaata
     5941 catcacgaca catttctaac gccagttcca ccagatacat ggcacggggg atatccaccg
     6001 ccggtgttcc gctcatgttt ggtgccatat cggacatgac aacctggact ttgctgtcgc
     6061 caacgcgctc cagcagtgct ttcatcacca gttcatcacg aaaatcgccc tgaagaaagt
     6121 ccacaccaac gataggatcc ataggtaaaa gatcgcaagc gatgatgcgg cctttgccgc
     6181 caatttgggt gaccacatat tgtgaccaac cacccggagc agcaccaagg tcgacaaccg
     6241 tcattcccgg tttaaagagt ttgtcacttt gctgtatttc atcaagttta aaccaggcac
     6301 gggaacgtaa cccctttttc tgtgcctgtt gaacatattt atcgctaaag tgttcctgaa
     6361 gccagcggct ggagctggca gaacgcttct tacctgtcat ttaactttcc catggggata
     6421 actcatcgta accaattgcg taaattttta cgcgcctatt tggtgatata tgggagatgg
     6481 cggtagaatg acccgttttc aatcccaacg taagcaaaaa atacgatgaa tctgagtact
     6541 aaacaaaaac agcacctgaa aggtctggca catccgctca agccagttgt tctgcttggc
     6601 agtaatggtt tgaccgaagg ggtgctggcc gagattgaac aagcgttaga gcaccatgaa
     6661 ctcatcaagg tgaaaatcgc caccgaagat cgcgaaacaa aaaccttgat cgtggaagct
     6721 atcgtgcgcg aaaccggcgc ctgtaatgta caggtcatcg gtaaaacgct ggtgctttat
     6781 cgcccaacta aagaacgtaa aatctcgctg ccacgctaag attatcctaa agttacacac
     6841 atttgctgtg taaaacgagg ggttttccgc aggcaggaga gcaaaatgcc acgctctgtt
     6901 cgttgataaa aggccgcata gcggcctttt tcctttcttt acaatacatc aacatcttga
     6961 tgtattgggt aattcttaca ggtattccac cttaattact tcaaattcta cttcgccgcc
     7021 cggcgttttg atgaccacaa catcatcttc ttctttgccg atcaggccac gagcaatagg
     7081 cgagtttaca gaaatcaggt tttgtttaaa gtcagcttcg tcatcgccaa cgatgcgata
     7141 agtctgttct tcgtcagaat ccagattcag caccgttacg gtagcaccaa aaataacgcg
     7201 cccattgttg ggcattttgg tgacatcaat cacctgcgcg ttcgacagct tggcttcgat
     7261 gtctttaata cggccttcgc agaaaccctg ctgttcacga gctgcgtggt attcggcgtt
     7321 ttctttcagg tcgccatgct cacgcgcttc cgcgatagca gcaatgattt caggacggcg
     7381 cacagatttc agaaaatcca gctcttcgcg taatttttca gcgccgcgta aggtcatcgg
     7441 aatagtttgc atttgttata cctcttgaat attcctgatg gggcaagtct tcacccaccc
     7501 cggctgttcg gcctgcccgg cataactcct caccgggacc agaagcaaaa aaataccgac
     7561 ccgggtacaa gtcctaggtc agctacaatt cacattttga tagtcatttt accctgaagt
     7621 tcccgaaggg tcatcgttta ctttataggg cgttgcgccg tagtatgacg gctcgattcc
     7681 aggttgttag cgcgagatta tgcgattttc cagatttatc atcggattga ccagctgtat
     7741 agcgttcagt gttcaggccg cgaatgttga tgagtacgtt actcaactcc ccgctggtgc
     7801 caaccttgcc ctgatggtgc aaaaagtcgg cgcgtcggcc cccgctattg attaccacag
     7861 tcagcagatg gcgctgcccg ccagtacgca gaaagtgatt actgcgctgg cggcgttgat
     7921 tcaactcggc cccgattttc gtttcaccac gacgcttgaa accaaaggca atgtggaaaa
     7981 cggcgtactt aagggtgact tagtggcgcg atttggtgcc gatccgacgt taaaacgtca
     8041 ggatattcgc aatatggtcg cgactttgaa aaaatctggc gtcaaccaaa tcgatggcaa
     8101 tgtgttgata gatacctcca ttttcgccag ccacgataaa gcccccggct ggccatggaa
     8161 tgacatgaca caatgcttta gcgctccgcc tgccgctgcc atagttgacc gcaactgttt
     8221 ctccgtctcg ctctacagtg ccccaaaacc tggtgatatg gcttttatac gcgtggcatc
     8281 ttattacccc gttacgatgt tcagccaggt acgcaccctc ccccgaggtt ctgccgaagc
     8341 gcaatactgc gaactggatg tcgtaccagg cgacctgaac cgctttacgc tgacgggatg
     8401 cctgccacaa cgttctgagc cgctcccgtt ggcttttgcc gtgcaggatg gagccagcta
     8461 tgccggggca attctgaaag atgagttaaa acaggcaggt atcacctgga gcggaacact
     8521 gctgcgccag actcaggtta acgaacctgg aacggtagtt gccagtaaac agtcggcccc
     8581 gttgcacgat ctgcttaaga ttatgctgaa aaagtcggac aacatgatcg ccgatacggt
     8641 tttccgcatg ataggccatg cgcgcttcaa tgtgcctgga acatggcggg ccgggtcgga
     8701 cgccgtgcgt cagatcctgc gccagcaagc cggtgtcgat attggaaaca ccattattgc
     8761 cgatggttca gggctttcgc ggcataacct gattgccccc gccaccatga tgcaggtgtt
     8821 gcaatacatt gcccaacacg acaatgaact taactttatc tccatgctgc cgctggcggg
     8881 ctatgatggc tctttgcagt accgtgcagg tctgcatcag gcgggcgtgg atggaaaagt
     8941 ctcagcgaaa accggttcgt tgcagggggt atataacctg gcgggattca ttaccacagc
     9001 gagcgggcaa cgaatggcgt ttgtacaata tctttctggc tatgcagtag aaccggcgga
     9061 tcagcgtaat cgccgtattc cgttagtgcg ttttgaaagc cgtttgtata aagatattta
     9121 tcagaacaat tagtcaaaaa gaaaccccgg cacatggccg gggttttgct gattaacgct
     9181 tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcatcatcc cagtcctcat
     9241 catcctcttc agcaatctct tcaagctgct ggcgatgata atcatcccac atgaattcga
     9301 ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg ataaaggtca
     9361 tcacatccca gcagagatct ttcacgccca gtccactcgc cgcagagatc agataatatt
     9421 tatcttccca gcccagcgct tcagcaatcg ctttcgcttt ctcttcagct tctgccttat
     9481 ccagcagatc gatcttattg aacactaacc aacgcggttt cgccgccaga tcctggctgt
     9541 atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg
     9601 gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc aggaagcgga
     9661 tacccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg gcaacaacga
     9721 agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg gtaaacggat
     9781 aatccgccac tttcggttta gccgccgata ccgcgcgaat aaaggtcgat ttacccgcgt
     9841 ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc agctcacgct
     9901 tatcacccgg cgtaccgttg gttttctgcc gtggtgtacg gttaacggac gatttgaaac
     9961 gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga ccgtgtttag
    10021 tcatatcgcc catggtttca ccagtaccct ggtcgattac acgcgtacct accggcactt
    10081 taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg ttctgaccgc
    10141 gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg ttttcgtcgg
    10201 cttctatcca tacgtcacca ccatcaccac cgtcgccgcc atccgggccg cctttcggaa
    10261 tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca acgaccagaa
    10321 tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct gcgcggggtt
    10381 gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca atggcggaat
    10441 acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt aacatcggtc
    10501 tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc gtaaacagtg
    10561 gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca
    10621 gggcgccata tcctaccaat gtattcagtc cgcaaaaaat taaacatgcg agctgccagt
    10681 ggctaagctg cgctatcact cctggctttg ccagagggaa gagcgcaatt gtacataaac
    10741 tgtacagcaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc ttttgcgcca
    10801 cgccataact cacccaaacc gtcgccgcac cgaccccaaa gatgactccc caggtgtaat
    10861 cagtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc aggccgctca
    10921 ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg ataaatacgc
    10981 tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca
    11041 ggtattgcaa ggatgcgctg aacaggatga agttcccaaa cagcccggcg gtcgccactg
    11101 ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca
    11161 ccgcaagaat ggcacccagg ccaatactcg ccatcaagaa acggtaaaac acgattgtcg
    11221 gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc cagcaaattg
    11281 ctgtggtgag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg tattccctgc
    11341 aaagcgcatt tgcggattaa cgtcgcacca tgctcatttt taccggttat cgaatgtaaa
    11401 aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa acttattcag
    11461 cttcgatgct gataaattta tggtttttcg ggcctttaac ttcgaatttc actttaccgt
    11521 ctgctttagc aaacagagtg tggtcacgac cgcaacccac gttagcgcca gcgtggaatt
    11581 tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca ccgaaacgct
    11641 taacgcccag gcgtttagct tctgaatcgc gaccgttacg tgtggagccg ccagcctttt
    11701 tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc
    11761 agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac gaaacttaac
    11821 gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta cgccgccatc
    11881 aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca cttcagcgaa
    11941 ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct gaccttcgct
    12001 tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact ccgcttccgc
    12061 gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc gcgaatatta
    12121 cgcaaaacgc acgcctttga caagtgctac agtcaatata cgaagaaaaa aaacacaact
    12181 tgtacggtaa cgtttatctg tgccattttt tcagtacaat caccctatat tcctaaccat
    12241 aaaccctaag ttgcttttgt tcacagtaag gtaatcgggg cgagaagccc ggcttttgcg
    12301 atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt taatgcggca
    12361 atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc
    12421 agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg agctgttggc
    12481 tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca cacggcgact
    12541 ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc taccgccaac
    12601 gctgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc
    12661 cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga agctgtaaac
    12721 gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga catcactgaa
    12781 gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag
    12841 tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca ggattatggg
    12901 cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa tgccgatggc
    12961 gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg
    13021 ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac cgccatcgaa
    13081 cagggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc ttgtggatct
    13141 cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc agcgttacag
    13201 gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa
    13261 cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgt gggatgattc cagtaaattc
    13321 cataaaaaca ctaattcagc gctatacacc tgaaaaaagg gcgatgaatc tttatatatt
    13381 ctgaacattc acactcttta caggaacttt ttagagcaat aggccatcag gagtatagtg
    13441 atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg aggaaagatg
    13501 gaaagtaatt tcattgactg gcatccccct gacatcattg caggtttgcg caagaaagga
    13561 acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct ggcaaacgca
    13621 ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg aactgacccc
    13681 tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat cgacagaacg
    13741 cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta gccccgcgaa
    13801 cggggctgcc agctctcaga cgattactcg cctttcacac gcttaatatt tgcacctaaa
    13861 gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat acgatcaacc
    13921 accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc acgcagatcg
    13981 gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac ggtattgctt
    14041 tcgatttcgg catgcgcgcc catacggcta agctccggca catgcataaa gcggttttca
    14101 aagaccgttt cggtgatgaa tccggtccct tctgccacca ggttcaacag cgtgaactgg
    14161 gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac agccttcgga
    14221 cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc cgctccagcg
    14281 tcacgcagtt tcgccagcac ggcgtcgaga gtatctggct gcgcgttacg gcagataatt
    14341 ttgccgcgag aaatcgccgc cgccaccagg aaagtaccgg tttcgatacg atccggtagc
    14401 acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg atcggtgccc
    14461 tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac gatttccggt
    14521 tcacgcgctg cgttttcaat aatcgtggta ccttccgcca gggttgcagc acacatgatg
    14581 gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt cagacgacca
    14641 tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa ttgttcgagg
    14701 ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg cagtgaaact
    14761 tgcccctgac caaagcgcgc taccagcggc cccagtgccc agatagaagc acgcatggtt
    14821 ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat atgcacagaa
    14881 ccattacgtt ctactttcgc acccagctga cttagcagct tcattgatgt atcgacgtct
    14941 ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagagc ggcaaaaagg
    15001 ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag cttcgttggc
    15061 ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat atccgctacc
    15121 ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca ctccgcaggg
    15181 gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc catcagcggg
    15241 ccatagaccg tctgctgttt tttaacccga ctcatgccgt caaacaactc acccacggca
    15301 ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga gagagcgttc
    15361 atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag ttaataaacc
    15421 agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc tcgctgataa
    15481 atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg ccatatccgg
    15541 cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt cgccagggta
    15601 tacactttgt cgtttacccc ctgaagcgtc acattgttgc cctgcttttt cgccagatcg
    15661 ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg gctaagatcg
    15721 atgcaggtaa tccccttcac cgcttcctca cgcatttccc aaagcggtag caaaacgtcc
    15781 tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct cagtgactcg
    15841 ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc agttgcgcag
    15901 tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt ttggtggtga
    15961 tcatactgac gccttcagca atcatgtcgt aagcctgcca gttgcccgtc tgggagtttt
    16021 tacgccactg gaagtccaga cgcaccggcg ggcggccatt cggatcaata atggtaacgc
    16081 gaatcggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga taggtttgac
    16141 cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg aaagcggcaa
    16201 agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc accagcgcac
    16261 cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta cgcagataat
    16321 ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt ttctgcgccg
    16381 cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca ctcagcggtg
    16441 caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc tcctgaaatt
    16501 atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca gctggcgcat
    16561 cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca atgagatctt
    16621 ccagcaccat cgcagactta gtgtcctgaa ttgtatcgcc atccttcagg atagcagtcc
    16681 ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttccccc agcaggccgg
    16741 aagtacgaat gctcagcgaa ctggtatctg gaatgtggtt ataacgttgt tcaatttcca
    16801 gcgttacgcg tggcagatag gttttcgggt ccagcgtaat atccgccacc cggcccacaa
    16861 caacgccacc aatactgacc ggagagcgcg ctttcaggcc gccaatgtta tcgaacgtcg
    16921 cataaagcgt gtaggtcggt tcagtacgta tggacgtcac gttcgccgcc ttcaggcaaa
    16981 caaacagagc cgccagcagt gctgctaata aaaagatacc cacccaaatt tcattttttt
    17041 tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc cagccccaga
    17101 acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc ggcagacgtc
    17161 gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc gaacaccacg
    17221 ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg cattgccgac
    17281 cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagagatcc gccccagatc
    17341 cccacggcga cgaaaataac cgtcaacagt ggtaatgaaa taaccccagc ccagaaacgg
    17401 ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga gagttgctct
    17461 gtagcgcgca tcaggccgat ttctgcggtt agcgccgaac cagcacgccc ggcaaacaac
    17521 aacgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag catacccaga
    17581 ctggtttccg cactataagt ggtcagaacc agataacctt gcagccccaa caccattccg
    17641 atgaacacgc cagaaaccac aataatcagc atcgacagga cgccgacatt atagagctgg
    17701 cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc attgaataac
    17761 attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc gagcgacgcc
    17821 agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt gataatcgcc
    17881 ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact gacgtacgcg
    17941 cggatcagga ttcgcctgca acgcctgggc actgccatga gcgacaattt ttttgtccgc
    18001 caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag aaaccaccac
    18061 acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta cgcccatggt
    18121 gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct ccagcgcaat
    18181 cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag gcattagttt
    18241 agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat gcaacaatgg
    18301 cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata cgttcatatc
    18361 agtgaacaac gccccggact gaaataacat gctcatccgt ttgcgcactg tatacaggcg
    18421 cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat gatctggtgc
    18481 gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg gccccatgat
    18541 cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc agcgattgcc
    18601 acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct ccataattca
    18661 cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga acccaacata
    18721 tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac tttttagccg
    18781 cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg gaccggcgag
    18841 tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt gttggtttac
    18901 ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt tgccgaacct
    18961 ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca tcgttaccag
    19021 aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc gttggtacag
    19081 ccctcggctc aaacattatc aatattttgc tgatcctcgg tctggctgcg ctggttcgtc
    19141 cttttaccgt ccattccgat gttctgcgtc gtgaattacc cttaatgttg ttggtcagcg
    19201 tagtggccgg ttccgtactc tatgacggac aacttagtcg cagcgatggt atctttctcc
    19261 tctttctggc tgtgctatgg ctgctgttca ttgttaaatt tgcacgtcag gctgaacgtc
    19321 aggggactga cagcctgacc agagagcagc ttgcagagct gccgcgtgac ggcggattgc
    19381 ccgtcgcgtt tttatggctc ggcattgcgc ttatcatcat gccagtggcc acgcggatgg
    19441 tggttgataa cgccacggtg ctggcgaatt actttgccat cagcgagttg acgatgggtc
    19501 tgacggcaat tgctatcgga accagcctgc cggaactggc aaccgcaata gcgggggttc
    19561 gcaaaggtga aaacgacatt gctgtcggaa atatcattgg cgcaaacatt tttaatattg
    19621 tcatcgtgtt gggtttaccc gcgctgataa cgccaggaga gattgatcca ttggcgtaca
    19681 gtcgtgacta cagcgtgatg ttgctggtga gcattatttt tgcgttgctg tgctggcggc
    19741 gctccccgca accgggccgt ggtgtagggg tattattaac tggcggattt atcgtatggc
    19801 tggcgatgtt gtactggtta tcgccaatac tcgttgaata actggaaacg cattatgtcg
    19861 cacgtagagt tacaaccggg ttttgacttt cagcaagcag gtaaagaagt cctggcgatt
    19921 gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc agaatttcac gcttgcctgt
    19981 gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa atcggggcac
    20041 atcgggcgca aaatggccgc aacgtttgcc agcaccggta caccttcatt tttcgtccat
    20101 ccaggtgaag ccgcgcatgg tgatttaggc atggtcaccc cacaggatgt ggtgattgct
    20161 atctctaact caggtgaatc cagcgaaatc acggccttaa ttccagtgct taagcgtctt
    20221 cacgtaccgt taatctgcat caccggtcgc ccggagagca gcatggcgcg cgccgcagat
    20281 gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc accgaccagc
    20341 accaccaccg ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc
    20401 ggctttactg ctgaagattt tgcgctctca cacccaggcg gcgcactggg tcgtaaactt
    20461 ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg
    20521 gccagtctgc gtgacgcatt gctggaagtt acccgcaaaa atcttggtat gactgtcatt
    20581 tgcgatgaca atatgatgat tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc
    20641 gatatgggcg tggatgttcg tcagttaagt attgccgatg tgatgacgcc ggggggaata
    20701 cgcgtgcgcc ctggcattct ggccgttgag gcactgaact taatgcagtc ccgccatatc
    20761 acctccgtga tggttgccga tggcgaccat ttactcggtg tgttacatat gcatgattta
    20821 ctgcgtgcag gcgtagtgta aagattcaag gataaacaac aatgagcaaa gcaggtgcgt
    20881 cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat agcaaaagca gagaacattc
    20941 gtctgctgat cctcgacgtt gatggcgtac tgtcagatgg cctgatttat atgggcaata
    21001 acggcgaaga gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca
    21061 cctctgatat tgaagtcgct atcattaccg ggcgaaaggc taaactggta gaagatcgtt
    21121 gtgccacatt ggggatcact cacttgtatc aggggcagtc aaacaaactg atcgccttta
    21181 gcgatctgct ggaaaaactg gcgattgccc cggaaaatgt ggcttatgta ggcgatgatc
    21241 tcatcgactg gccggtaatg gaaaaagtgg gtttaagcgt cgctgtggcc gatgctcatc
    21301 cactgttgat cccgcgcgcc gattatgtga cgcgcattgc tggcggtcgt ggcgcagtgc
    21361 gagaagtttg cgacttatta ctcctggcgc agggcaaact ggatgaagcc aaagggcaat
    21421 cgatatgagt aaagccagac gttgggttat cattgtgcta tcactggcgg ttctggtgat
    21481 gatcggcatt aatatggccg aaaaagacga taccgcccag gtggtcgtca acaacaatga
    21541 tcccacctat aaaagcgagc atacggacac gctcgtctat aacccagaag gggcactaag
    21601 ctatcgattg attgctcaac acgttgaata ttattccgat caggccgttt cgtggtttac
    21661 gcagccggta cttaccacgt ttgataagga taaaatcccg acatggtccg taaaagcaga
    21721 taaagccaag ctgaccaatg accggatgct ctatttatat ggacacgttg aagtcaacgc
    21781 acttgtgccg gactctcaac ttcgcagaat cacgacggat aacgcgcaga tcaatctggt
    21841 gacgcaggat gttacctctg aagacctcgt cacgttatac ggaacaacat ttaactccag
    21901 cggtctgaaa atgcgcggca acttacgcag caagaacgcc gagctgattg aaaaggttag
    21961 aacatcctat gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt gccagctcac
    22021 ttctggccgc cagcattccg gcatttgccg taaccggaga cactgatcag ccgatccaca
    22081 ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt ggttaccttt accggtaatg
    22141 tcatcgtcac ccagggcacc atcaaaatta atgccgacaa agtggtcgtt acccgtccgg
    22201 gcggcgaaca aggtaaagaa gtgattgacg gctacggtaa accggcaacg ttctaccaga
    22261 tgcaggacaa cggtaaaccc gttgaaggtc atgcttccca gatgcactac gaactggcaa
    22321 aagattttgt cgttctgacg ggtaatgctt atctgcagca ggtcgatagc aacattaagg
    22381 gcgataagat cacttacctg gtgaaagagc agaaaatgca ggctttcagc gacaaaggca
    22441 agcgcgtaac aaccgttctg gtgccgtcgc agctgcagga caaaaacaac aaaggccaga
    22501 ccccggcaca gaagaagggt aattaattcg ttatggcaac attaactgca aagaaccttg
    22561 caaaagccta taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc aactccgggg
    22621 aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac caccactttc tatatggttg
    22681 ttggcattgt gccgcgcgat gcgggcaaca tcattattga tgatgacgat atcagtctgc
    22741 tgcctctgca tgcacgcgcg cgccgcggta tcggctatct gccacaggaa gcctccattt
    22801 tccgtcacct cagcgtttac gataacctga tggcggtact gcaaattcgt gacgacttgt
    22861 ctgctgaaca acgtgaagac cgcgcgaacg agctgatgga agagtttcac attgagcacc
    22921 tgcgtgacag catggggcag tcactctccg ggggtgaacg tcgccgtgta gaaattgccc
    22981 gcgcactggc tgcgaatccg aaatttattc tactcgacga accgtttgcc ggggttgacc
    23041 cgatctcggt tatcgacatt aaacgcatca ttgaacacct gcgcgacagc ggcctgggcg
    23101 tactgatcac tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg
    23161 tcagtcaggg gcatttgatt gcccacggca cgcctacaga aatcttacaa gacgaacacg
    23221 ttaagcgtgt ataccttggg gaagacttca gactctgata gggtagaagt ttgcgacgtt
    23281 ttagcaggag agtacgattc tgaacatgaa gcaaggtttg caactcaggc ttagccaaca
    23341 actggcgatg acgccacagc ttcaacaggc gattcgtctg ttgcagttgt cgacgctgga
    23401 acttcagcag gagctacagc aggcgctgga gagtaatccg ctgcttgagc aaatcgacac
    23461 tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa acgctggaca ccgccgacgc
    23521 gctcgaacaa aaagagatgc cggaagagct gccgctcgat gccagttggg acaccattta
    23581 caccgctggt acaccatccg gcaccagcgg tgactacatt gacgatgagc tgccggtcta
    23641 tcagggcgaa acgacgcaga ccttgcagga ttacctgatg tggcaggtcg agctgacacc
    23701 gttttccgac actgaccgcg ctattgctac ctctatcgtc gatgccgttg atgacaccgg
    23761 ttatctgact gtcccgctgg aagatattct cgaaagtatg ggcgatgaag agatcgacat
    23821 cgacgaggtt gaagccgtcc ttaagcggat ccaacggttt gatccggtcg gtgtagcggc
    23881 aaaagatctg cgtgactgcc tgctaatcca actctcccaa ttcgataaaa ccacgccgtg
    23941 gctggaagag gccagactga tcattagcga tcatctcgat ctgttagcca atcacgactt
    24001 ccgcacttta atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct
    24061 gatccagtcg ctcgatccgc gccccgggca gtcgatccag actggcgaac ctgagtatgt
    24121 cattccagat gtgctggtgc gtaagcataa cggtcactgg acggtagaac tcaacagtga
    24181 cagcattccg cgtctgcaaa tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa
    24241 cgatggtgac agccagttta tccgcagcaa tctgcaggat gccaaatggt tgatcaagag
    24301 tctggaaagc cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca
    24361 agccttcttt gagcaaggtg aagaatatat gaaaccgatg gtactggccg atatcgccca
    24421 ggctgtcgaa atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag
    24481 tccacgaggc atttttgaac tgaagtattt cttttccagt cacgtcaata ccgagggcgg
    24541 cggcgaagct tcctccacgg cgattcgtgc gctggtgaag aaattaatcg cggcggaaaa
    24601 cccagcgaaa ccgttgagcg acagcaagtt aacctctttg ctgtcggaac aaggtatcat
    24661 ggtggcacgc cgcactgttg cgaagtaccg agagtcttta tccattccgc cgtcaaacca
    24721 gcgtaaacag ctcgtttgac ccaaccgata aggaagacac tatgcagctc aacattaccg
    24781 gaaataacgt cgagatcacc gaggcactgc gcgaatttgt tagagccaaa tttgccaaac
    24841 ttgagcaata ttttgaccga atcaaccagg tctatgttgt tctgaaagtg gagaaagtca
    24901 cccacacctc agatgcaaca ctgcatgtaa acggtggtga aattcatgcc agcgcggaag
    24961 gtcaggacat gtacgctgcc attgatggct taattgataa gctggcacgc cagttgacca
    25021 aacataaaga taaactgaaa caacactaat tgtccgggca attagcatgt gcatggcggc
    25081 ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct ccgagcctgt tccactgttt
    25141 gagtgggcag gttcttaggt gaaattatga caaataatga tacaactcta cagcttagca
    25201 gtgttcttaa cagggaatgt acgcgaagcc gcgttcactg tcagagcaaa aaacgcgccc
    25261 tggaaatcat cagcgagctg gcggcgaaac aacttagcct gccgcctcag gtggtttttg
    25321 aagctatcct gacgcgggaa aaaatgggca gtaccggtat cggcaatggt attgccattc
    25381 cgcatggcaa actggaagaa gatactctgc gcgccgttgg cgttttcgtt cagctcgaaa
    25441 cgcctatagc tttcgatgcc atcgacaacc aaccggtgga cttacttttt gccctgctgg
    25501 tgccggcaga ccaaactaaa acgcacctgc atactctgtc gctggtggcg aaacgtctgg
    25561 cggacaaaac catctgccgc cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa
    25621 tcattacgga taccgaaggt actccggatg aagcgtagtt attcggtaat gtctctttta
    25681 gacgttgtga ggagaaacag tacatggtac tgatgatcgt cagcggacgt tcaggttcag
    25741 gtaaatctgt cgccctgcgt gcgctggaag atatgggttt ttactgcgtg gataaccttc
    25801 ccgtagtgtt gttacccgat ctggctcgaa ccctggccga tcgcgagatt tctgccgccg
    25861 tcagcattga tgttcgtaat atgccggagt caccagaaat attcgaacag gcgatgagta
    25921 acctgcctga cgctttctca ccgcaactcc tgttcctgga tgccgaccgt aataccttaa
    25981 ttcgtcgtta cagtgacacg cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg
    26041 aaagtgctat cgacaaagaa agcgatttgc tggagcctct gcgttcgcga gcggatctga
    26101 ttgtcgatac ctcagaaatg tccgttcacg agctggctga aatgctgcgt acccgtctgc
    26161 tgggtaaacg tgaacgcgaa ctgaccatgg tctttgagtc tttcggcttc aaacacggta
    26221 tccctatcga tgcagattac gtctttgacg tgcgcttctt gccgaacccg cactgggatc
    26281 cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca
    26341 cagaagtaca caattttatc taccagacgc gaagctatct tgagctatgg ttacctatgc
    26401 tggaaaccaa caaccgtagc tacttgacgg tcgccattgg ttgtaccggc gggaagcacc
    26461 gttcggtgta tattgcagag caactggcag actacttccg ctcgcgcggt aaaaacgtcc
    26521 agtcacgcca tcgtacgctg gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat
    26581 cacaaacaag ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg
    26641 ttttgacgct gaagtgctgt tacgtaatga cgaaggcacc gaggccgaag ccaacagcgt
    26701 tattgcgctg ctgatgttgg attctgccaa aggacggcag attgaagttg aagcgaccgg
    26761 cccacaggaa gaggaagcac tggccgccgt tatcgccctc tttaactctg gttttgatga
    26821 agattaatct tcatcacttt gacatacaac tcccttcaaa ctccccccga caataagaaa
    26881 atcacgtact gaaatcgttc tcaatcaacg tcatttgtac attttgtgcg cttttcactt
    26941 tcagaagaac cttaagaaaa ccttaagagg cattgtttag gttttgttta agttaatcga
    27001 ccatactgga gatcgtcaga aaatatttcc aggagatggc atgattcgct tatctgaaca
    27061 aagtcccctg ggcaccggaa gacatcggaa atgttatgcg catccggaag atgcccaacg
    27121 ctgtatcaag attgtctacc atcgtggcga tggtggcgat aaagagatcc gccgtgagtt
    27181 aaagtactac gcgcatcttg gtcgccggtt aaaagactgg agtggaatac cgcgctatca
    27241 cggtaccgtt gagacggatt gcggaaccgg ttatgtctac gatgtgattg cagattttga
    27301 cggcaaaccc tccattacgc tgaccgaatt tgctgaacaa tgccgttacg aagaagacat
    27361 tgcacaactg cgccagctac tgaaacagct gaagcgttat ttgcaggata accgtatcgt
    27421 gacgatgtcg ctgaagccgc agaatatcct ctgtcaccac atcagtgagt cagaggttat
    27481 cccggtcgtc tgcgacaaca ttggtgaaag cacattgatt ccactggcga cctggtcgaa
    27541 gtggtgttgt ctgcgtaagc aagagcggtt atggaaacga tttatcgccc aaccggcgct
    27601 ggctattgcc ctgcagaaag atctgcaacc tcgtgagagc aaaacgctgg ccctcacttc
    27661 gcgcgaagct taatccagct ggtgttgttg cataaacggc tcaccgccta actgatacat
    27721 ctgccgtaaa atccacgcct gacggctacg cacgtagccc gatggcgagg agactttgaa
    27781 acgaagtgga ttaggtaata cagctgccag taatgcagct tccgaccggg taagtttgct
    27841 cgcgggtttg tggaaataac gttgtgccgc agcttcgacg ccaaacacac cgtcgccaaa
    27901 ttcggcgata ttcaggtaaa ccgtcaggat acgctttttg ctccagactg tttctatccc
    27961 cagcgttaat ccagcttcca gcccttttcg tacccagcta cgcccatccc ataaaaagag
    28021 atttttggcc gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc
    28081 gttgtgcgcc agggctttct caatggaagc gacatcaaag ccccagtgct caggaaattt
    28141 ctgatcttct gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac
    28201 ccagtcagaa tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cctgtcgctc
    28261 gaccattacc gctgagaagg gaacaggcgc aacactaaac aacgcgatgc ccccgcccca
    28321 gaaaacggcg aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt
    28381 taagcggctt ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg
    28441 ctcgccgctt ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac aatcttattg
    28501 tcttcatcaa ccacgatatc atcgacagga cagggcacat gctccgcgcc catctcttcc
    28561 agcacttctg cggtatcgat atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt
    28621 ttcggcagca tcgccggggc aatacacata aaaccaagcg gttttccggc ctgatgcatc
    28681 gcttgtgcca gcgcctttaa ttcacggtca acggtgcatt cgctaccaag agtggcaaaa
    28741 ttgcttaaat tcttcgccgc gccaaacccc cccggcacaa tcaacgcatc cagttcagtg
    28801 gcatcggcct gggccagagg acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc
    28861 acgttgcgcg tttccgtcat cgcttcgcca gttaaatggt tgataacatc aacctgctgc
    28921 ttatccggtg caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac
    28981 accgcttcat gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt
    29041 ttcttcattg tgatcatcct ttcgcaattg actgaaactc attaattttt ttaataaaaa
    29101 tggtacgcat cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta
    29161 actgcgtgcg gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc
    29221 gcagccaaga tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca
    29281 ttttttagtg gcttttgcca cccacgcttt cagcacttct acgtcgtgac gccactcttc
    29341 tttcatctct tcaatccatt caccgacgtt atcttcccag gcaggaaggt caggagactg
    29401 aatttgctga cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat
    29461 tttatgtcct tcctcaacaa tgcctttttt atcctgcgcc gtcagattcg actccagcac
    29521 gctgacatag cccggcatca tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg
    29581 tcctacaagt tcgagatact gttccagcat gagaatatcg agcaatgctt ctgatttact
    29641 gttctcttct gtcgtcaccg tactctcctc atcatcctgg gtatcccaga atttcttgat
    29701 catcgcggtt agcgccggaa ccgaaagcgg cttactcagc acatcatcca ttccagcatt
    29761 gaggtactct tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc
    29821 ctcgcgcgga taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa
    29881 ctgaatatcc agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt
    29941 gccggtcatg gcgacatcaa cgctgttacc taatttttcc agcacagaac gcgcaacaat
    30001 cacgttcagt tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc
    30061 ttcatcaaac gcatcatcga cctcttctgc taccgacggt gcgtggatcg tcaacgtaaa
    30121 ggttgaacct ttgccctgtt cgctggtaac cgtaatatcg ccgcccatat ttttcgccag
    30181 acgacgagaa acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc
    30241 tttcacctgg taatacatgg cgaaaatttt atccagctca tcctgcggaa tgccgatacc
    30301 agagtcttcc acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac
    30361 ctggccttgc tgggtgaatt tgacggcgtt actgatgagg ttccacagga tctgccgtaa
    30421 acgcgtcccg tcggtaatga cctgatgcgg taatggcagc gtcggctcca ggttaaagcg
    30481 caatcctttt tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt
    30541 gaaatcaacc ggttgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat
    30601 aatatcgtta aagatattcc ccagcgtgac ggccgaaaca tggatggtct tgagatattt
    30661 ttcctgctcg gcggtgagtt cggtatccag cagaatgcgg ctcagaccga cgataccgtt
    30721 cagcggtgta cgcaattcgt gactgatggt ggagataaac gtcgttttgt cgcggctggc
    30781 ccgttcaagc gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa
    30841 accgtgacgt ttacccacgc ggtcgtagta cggcacttta cggatttcaa agcaggcttt
    30901 gcgcccgtcc gggtaatcca gccactgttc ataggtcagt gacacattat gacggaacac
    30961 tttttcatcg gtttcaatga cttttgcggc ggcttccggt gagtaaacat cagcaggttt
    31021 caggtgaacc agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc
    31081 ggaaaactct ttatcttcgt tacgataaaa aaccaggtcg ggtgaagcat caaggaagga
    31141 acgtaagaat gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc
    31201 aattttcagt tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcaat
    31261 ttcctgattt agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc
    31321 gcgcatctcc tccagttttt gcaccagccg tgacagacgt tgtcgtgact cctccagttg
    31381 ctcgacgacc accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat
    31441 agaacgaata acatcaatgc tttcgacctg accatgcagc accatggtta ccgccatttg
    31501 taccacaatg gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc
    31561 taacttcatc atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga
    31621 attccttcac gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt
    31681 gtgagaaatg cggaattccc ctcaacgacc tactccgttg gaggtagcca cggacgtccc
    31741 agcgccgatc cctgtacgcc atgttcattc agatagcgat ccagctcgac cattcccgtc
    31801 catcgatttt cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg
    31861 cggtggtaga tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtgtaa
    31921 tcctccagtt caataccgtt caaacgcccc gcttcccagg ctttcgccat aatgctgcct
    31981 ttcacaatat gcagcggatg cagctttatg ccgtccacgc cggtttcaac cactcgctcc
    32041 agcgtttgca aacattcagc ctgcccttcg ccaggcaggc cgacaattaa gtgggaacac
    32101 accttcagcc cgcgctgacg tgccagctgg gttgtacgct gataacaggc aaaatcatgg
    32161 ccgcggttga tgcgatgcag tgttttgtcg tgggcggttt gtagccccag ctccagccac
    32221 acttcgtaac cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag
    32281 tccgggcggg taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac
    32341 atagaacgca gcacctgaac ttccgcaaag gtgctagtat acgcctgaaa gtaggccaga
    32401 tagcgtttag cgcggttaac taaattcgcc tggtgctcca gttgctcggc aatggaacga
    32461 tgctgctgcg cttcatcggc aaacgaggca acattacaga atgtgcagcc accacgcccg
    32521 atggtaccgt cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt
    32581 tgcccataac gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata
    32641 atctgataga ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg
    32701 atgacctgga tcaatcgtcc cggcctgctt ttatcaactg cataatcaat caaaattacc
    32761 gaaatttcat gcataatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc
    32821 tccttatgga ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat
    32881 aaaatgctgg ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag
    32941 tcaggaattg actgtttctc taacgacttc ccttttagcc ttaaagataa aattcattta
    33001 atttcagtta gttaataaag aattttgcgc taaagcacat ttctgtacca ataagcttgc
    33061 catttgacct gtatcagctt tcccgataag ttggaaatcc gctggaagct ttctggatga
    33121 gcagcctgct catcatattt atgcagtaat tgagatcccc tcttcaccgt attaaccgat
    33181 gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa cccccgtcgc
    33241 cacgctcttt ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca gagcctgggg
    33301 gaggttcacg atatgttgta cgataaatcc cttgagaggg ataactgtgg tttcggcctg
    33361 atcgcccaca tagaaggcga acctagccac aaggtagtgc gtactgcaat acacgcactg
    33421 gcccgcatgc agcaccgtgg cgcgattctc gccgatggta aaaccggtga cggttgcggc
    33481 ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt
    33541 ttagcaaaaa actacgctgt cgggatgctc ttcctgaata aagatcctga actcgcggct
    33601 gccgcacgcc gcatcgttga agaagagctg caacgcgaaa ccttgtcgat tgtgggctgg
    33661 cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc tctgccccgc
    33721 attgagcaaa ttttcgttaa cgctccggca ggctggcgtc cgcgggatat ggagcgccgt
    33781 ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc
    33841 tgtagcctgt cgaatctggt gaacatctat aaaggtctgt gtatgccggc ggatctgccg
    33901 cgcttttatc tggatcttgc ggacctgcgt ctggaatcgg ccatctgcct gttccaccag
    33961 cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc agccgttccg ctacctggcg
    34021 cataacggtg aaatcaacac catcaccggt aaccgccaat gggcgcgtgc gcgtacctat
    34081 aaattccaga cgccgcttat ccctgatctg cacgacgccg caccgttcgt caacgaaacc
    34141 ggctctgact ccagttcgat ggataacatg ctggaactgc tgctggcagg cgggatggat
    34201 atcatccgcg ccatgcgtct gttagtgcca cccgcctggc agaacaaccc ggatatggac
    34261 ccggaactgc gtgccttctt tgactttaac tccatgcata tggagccgtg ggatggcccg
    34321 gcgggcatcg tgatgtccga cggtcgtttt gccgcctgta acctcgaccg taacggtctg
    34381 cgtccggcgc gctacgtcat caccaaagat aagctcatca cctgcgcctc tgaagtcggt
    34441 atctgggatt accagcctga cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg
    34501 atggttatcg acacccgcag tgggcgtatt ctgcactcgg cagaaaccga tgacgatctg
    34561 aaaagccgcc atccatataa agagtggatg gagaaaaacg tccgccgact ggtaccgttt
    34621 gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg acgacgacac gcttgccagc
    34681 taccagaaac agtttaacta cagcgcggaa gagctggact ccgtaattcg cgtactgggc
    34741 gaaaacggtc aggaagcggt cggttcgatg ggcgatgata ccccattcgc cgtgctctcc
    34801 agtcagccgc gcattattta cgactacttc cgccagcagt ttgcccaggt gactaacccg
    34861 ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa
    34921 atgaacgtct tttgcgaagc agagggccag gcgcaccgtt taagctttaa atcgccgatt
    34981 ctgctctact ccgatttcaa acagctcacg acgatgaaag aggagcacta ccgcgcagat
    35041 acgctggata tcacctttga cgtcactaaa accacgctcg aagcgacagt caaagagctg
    35101 tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg
    35161 aatatcgcta aagatcgcct gccggttcca gccccgatgg cggttggcgc gatccagacc
    35221 cgtctggtcg atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc
    35281 cgcgatccgc accacttcgc cgtgttgctg ggcttcggcg cgacggctat ttatccatac
    35341 cttgcctatg aaacgctggg ccgcctggta gacacccatg cgattgccaa agattatcgt
    35401 accgtgatgc tcaactaccg taacggcatc aacaaaggct tgtacaaaat catgtccaaa
    35461 atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtctg
    35521 cacgatgatg tagtgggcct gtgcttccag ggggcggtca gccgcactgg tggggcgagc
    35581 tttgaagact tccagcagga tctgctgaac ctgtcgaaac gtgcctggct ggcgcgtaag
    35641 cccatcagcc agggcggcct gctgaaatac gtccacggcg gcgaatacca cgcttacaac
    35701 ccggacgtgg tgcgcacgct gcaacaggcg gtacaaagcg gcgagtacag cgactatcag
    35761 gaatacgcga agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt
    35821 acgccgggtg aaaacgcggt caacattgct gatgttgaac cggcaagcga actgtttaaa
    35881 cgctttgata ccgccgcgat gtctatcggc gcgttaagcc cggaagccca cgaggcgctg
    35941 gcggaagcga tgaatagcat cggcggtaac tcgaactccg gtgaaggcgg cgaagacccg
    36001 gcgcgctatg gcactaacaa agtgtcgcgc atcaagcagg tggcttctgg tcgcttcggc
    36061 gttactccgg cgtatctggt caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg
    36121 aagccgggcg aaggcggtca gttgccgggc gacaaagtca ctccttacat agccaaactg
    36181 cgctattcgg tgcccggagt gacgctgatc tccccgccgc cgcaccacga tatctactct
    36241 atcgaggact tagcgcagct cattttcgac ctcaagcagg ttaacccgaa agcgatgatc
    36301 tccgtgaagc tggtttccga accgggcgta ggcaccatcg cgactggcgt ggcaaaagct
    36361 tatgccgatt taatcaccat cgcaggctat gacggtggca ccggcgcaag cccgctgtcg
    36421 tcagtgaaat acgcaggttg cccgtgggag ctggggcttg ttgaaaccca gcaggcgctg
    36481 gttgccaatg gtctgcgtca taagatccgt ttgcaggtcg atggcggcct gaaaaccggt
    36541 gttgatatca tcaaagcggc gattctcggc gcagaaagct tcggcttcgg cactggccca
    36601 atggtcgcgc tcggctgtaa atatctgcgt atttgccacc tgaacaactg cgcaacgggt
    36661 gtggcaactc aggatgacaa actgcgtaag aatcactatc acggcctgcc gttcaaggtg
    36721 acgaattact ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca gctgggggta
    36781 acgcggctgg tggatctgat tggtcgcacc gacctgctga aagagctgga cggtttcacc
    36841 gccaaacagc agaaactggc gctgtcgaag ctgctggaga ccgccgaacc gcatccaggt
    36901 aaggcactct actgcaccga aaacaacccg ccgtttgata acggcctgct gaacgcgcag
    36961 ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat
    37021 atccgcaata cagatcgctc cgtgggcgcg tcgctttcag gctatatcgc ccagacgcac
    37081 ggcgatcagg ggctggcagc cgatcctatc aaagcgtact tcaacggcac cgcaggccag
    37141 agtttcggtg tatggaacgc gggcggcgtg gaactgtacc tgaccgggga tgccaacgac
    37201 tatgtcggta aaggcatggc gggcggctta atcgccattc gtcctccggt tggttccgcc
    37261 ttccgcagcc atgaagcaag tattatcggc aacacctgcc tgtatggcgc gaccggtggt
    37321 cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc
    37381 accgtggtag aaggcattgg cgacaacggc tgtgaatata tgacgggcgg tattgtctgt
    37441 attctgggta aaaccggcgt taacttcggt gcgggcatga ccggcggctt cgcttacgtt
    37501 ctcgatgaaa gcggtgattt ccgcaaacgc gttaacccgg aactggtcga ggtcttaaac
    37561 gttgacgatc tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag
    37621 catactggct ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt cgccactaaa
    37681 tttgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca ccgtagtcgt
    37741 agcgcagcag agttgcgcgt gcaggcgcag taaggggtag caacaatgag tcagaatgtt
    37801 tatcaattta tcgacctgca gcgcgttgat ccgcctaaga aaccgctgaa gatccgcaaa
    37861 attgagtttg ttgaaattta cgagccgttt tccgaaggcc aggccaaagc gcaggctgac
    37921 cgctgcctgt cgtgcggcaa cccatactgc gagtggaaat gtccggtaca caactacatc
    37981 ccgaactggc tgaagctcgc caacgagggg cgtatttttg aagcggcgga actgtcgcac
    38041 cagaccaaca ccctgccgga agtttgcgga cgagtctgcc cgcaagaccg tctgtgcgaa
    38101 ggttcctgca ctctgaacga tgagtttggc gcggtgacca tcggcaacat tgagcgttat
    38161 atcaatgata aagcgttcga aatgggctgg cgtccggata tgtccggcgt gaagcaaacc
    38221 ggtaaaaaag tggcgattat cggcgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg
    38281 acgcgtaacg gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg
    38341 accttcggta ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc
    38401 ttcaccggca tgggtattga attcaaactc aatactgaag tgggccgcga cgtgcagctg
    38461 gacgatctgc tgagtgatta cgatgccgtc ttccttggcg tcgggactta tcagtcaatg
    38521 cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc gttccttatc
    38581 gccaacacca aacagttaat gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg
    38641 gaaggcaaac gcgtggtggt ccttggcggt ggcgacactg cgatggactg cgtgcgtacg
    38701 tccgtgcgcc agggagcgaa gcacgttacc tgtgcctatc gtcgtgatga agagaacatg
    38761 ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac
    38821 gtccagccac tggggattga agtgaacggt aacggcaaag tcagcggcgt aaaaatggta
    38881 cgtaccgaaa tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt
    38941 tccgaacata tcgttccggc agatgcagtg atcatggcgt ttggtttccg tccacacaac
    39001 atggaatggc tggcaaaaca cagcgtcgag ctggattcac aaggccgcat catcgccccg
    39061 gaaggcagcg acaacgcctt ccagaccagc aacccgaaaa tctttgctgg cggcgatatc
    39121 gtccgtggtt ccgatctggt ggtaaccgct attgccgaag gtcgtaaggc ggcagacggt
    39181 attatgaact ggctggaagt ttaagctctg tcccagcgcc gcctccccag aaaagagtcg
    39241 gcgctgaaat tctcctttct ttctggaaag cgcctcgcat aatctgaaga aaaccaatgt
    39301 aatacgctca tctggattgt taccggtaga tgcccggcgt atagctgatt ccattattca
    39361 acgcagcgcc atataaaggg acagataatg gaatcgctct cagagagaac ctcaacaggc
    39421 taccagcaaa tccacgacgg tattattcac ctggtcgata gcgcccggac ggaaacggta
    39481 cgtagcgtta acgcgctgat gaccgcgaca tactgggaaa ttggccgacg aattgtcgaa
    39541 tttgaacaag gtggcgaggc aagggctgcg tatggtgcgc agctaatc
//
LOCUS       DAJTNA010000015        38361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTNA010000015 DAJTNA010000000
VERSION     DAJTNA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38361
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.015"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..725
                     /locus_tag="OO161_000668"
     CDS             <1..725
                     /locus_tag="OO161_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5461840.1"
                     /translation="EVRDFALILDEWLDKDMVSTARVNRGLWVDIYKEAQHAGEVPPG
                     WNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMR
                     FSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQK
                     QPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSERLYRAQGIDTKTLLGH
                     KVQATTDRYNDTRGQEWVKLVI"
     gene            892..1686
                     /gene="potC"
                     /locus_tag="OO161_000669"
     CDS             892..1686
                     /gene="potC"
                     /locus_tag="OO161_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5461841.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            1683..2723
                     /gene="potD"
                     /locus_tag="OO161_000670"
     CDS             1683..2723
                     /gene="potD"
                     /locus_tag="OO161_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5461842.1"
                     /translation="MKKWSRHLLAAGALALGAAHADDNNTLYFYNWTEYVPPGLLEQF
                     TKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKSKL
                     TNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYKGS
                     LLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPYME
                     GEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLINFL
                     LRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAASSI
                     YEEYYQKLKAGR"
     gene            complement(2879..3699)
                     /gene="cobB"
                     /locus_tag="OO161_000671"
                     /pseudo
     CDS             complement(2879..3699)
                     /gene="cobB"
                     /locus_tag="OO161_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
     gene            complement(3715..4626)
                     /gene="nagK"
                     /locus_tag="OO161_000672"
     CDS             complement(3715..4626)
                     /gene="nagK"
                     /locus_tag="OO161_000672"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5461843.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYTANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERAHHGDAGGMRGAAFLHLTD"
     gene            complement(4655..5899)
                     /gene="lolE"
                     /locus_tag="OO161_000673"
     CDS             complement(4655..5899)
                     /gene="lolE"
                     /locus_tag="OO161_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5461844.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLYVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(5899..6600)
                     /gene="lolD"
                     /locus_tag="OO161_000674"
     CDS             complement(5899..6600)
                     /gene="lolD"
                     /locus_tag="OO161_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5461845.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(6593..7792)
                     /gene="lolC"
                     /locus_tag="OO161_000675"
     CDS             complement(6593..7792)
                     /gene="lolC"
                     /locus_tag="OO161_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5461846.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8054..9127
                     /locus_tag="OO161_000676"
     CDS             8054..9127
                     /locus_tag="OO161_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5461847.1"
                     /translation="MKQKELWINQIKGLCIYPVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9255..12701
                     /gene="mfd"
                     /locus_tag="OO161_000677"
     CDS             9255..12701
                     /gene="mfd"
                     /locus_tag="OO161_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5461848.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            12845..13806
                     /gene="ldtC"
                     /locus_tag="OO161_000678"
                     /pseudo
     CDS             12845..13806
                     /gene="ldtC"
                     /locus_tag="OO161_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(13889..14146)
                     /gene="bhsA"
                     /locus_tag="OO161_000679"
     CDS             complement(13889..14146)
                     /gene="bhsA"
                     /locus_tag="OO161_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR5461849.1"
                     /translation="MKNVKILIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            14387..15019
                     /gene="comR"
                     /locus_tag="OO161_000680"
     CDS             14387..15019
                     /gene="comR"
                     /locus_tag="OO161_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5461850.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVKSALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15081..15620)
                     /locus_tag="OO161_000681"
     CDS             complement(15081..15620)
                     /locus_tag="OO161_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5461851.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(15847..17151)
                     /gene="ndh"
                     /locus_tag="OO161_000682"
     CDS             complement(15847..17151)
                     /gene="ndh"
                     /locus_tag="OO161_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5461852.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(17551..18093)
                     /locus_tag="OO161_000683"
     CDS             complement(17551..18093)
                     /locus_tag="OO161_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5461853.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18116..19141)
                     /gene="nagZ"
                     /locus_tag="OO161_000684"
     CDS             complement(18116..19141)
                     /gene="nagZ"
                     /locus_tag="OO161_000684"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5461854.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19152..19976)
                     /gene="thiK"
                     /locus_tag="OO161_000685"
     CDS             complement(19152..19976)
                     /gene="thiK"
                     /locus_tag="OO161_000685"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5461855.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(19960..20598)
                     /gene="lpoB"
                     /locus_tag="OO161_000686"
     CDS             complement(19960..20598)
                     /gene="lpoB"
                     /locus_tag="OO161_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5461856.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRYYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(20612..20989)
                     /locus_tag="OO161_000687"
     CDS             complement(20612..20989)
                     /locus_tag="OO161_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5461857.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(20992..21351)
                     /gene="hinT"
                     /locus_tag="OO161_000688"
     CDS             complement(20992..21351)
                     /gene="hinT"
                     /locus_tag="OO161_000688"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5461858.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            21685..23810
                     /gene="fhuE"
                     /locus_tag="OO161_000689"
                     /pseudo
     CDS             21685..23810
                     /gene="fhuE"
                     /locus_tag="OO161_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(23870..25303)
                     /gene="ptsG"
                     /locus_tag="OO161_000690"
     CDS             complement(23870..25303)
                     /gene="ptsG"
                     /locus_tag="OO161_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5461859.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILVGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(25598..26395)
                     /locus_tag="OO161_000691"
     CDS             complement(25598..26395)
                     /locus_tag="OO161_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5461860.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(26406..27410)
                     /gene="holB"
                     /locus_tag="OO161_000692"
     CDS             complement(26406..27410)
                     /gene="holB"
                     /locus_tag="OO161_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5461861.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(27407..28048)
                     /locus_tag="OO161_000693"
     CDS             complement(27407..28048)
                     /locus_tag="OO161_000693"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5461862.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(28038..29060)
                     /gene="yceG"
                     /locus_tag="OO161_000694"
     CDS             complement(28038..29060)
                     /gene="yceG"
                     /locus_tag="OO161_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5461863.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQELELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(29063..29872)
                     /gene="pabC"
                     /locus_tag="OO161_000695"
     CDS             complement(29063..29872)
                     /gene="pabC"
                     /locus_tag="OO161_000695"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5461864.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(29992..31233)
                     /gene="fabF"
                     /locus_tag="OO161_000696"
     CDS             complement(29992..31233)
                     /gene="fabF"
                     /locus_tag="OO161_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5461865.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(31321..31557)
                     /gene="acpP"
                     /locus_tag="OO161_000697"
     CDS             complement(31321..31557)
                     /gene="acpP"
                     /locus_tag="OO161_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5461866.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(31768..32502)
                     /gene="fabG"
                     /locus_tag="OO161_000698"
     CDS             complement(31768..32502)
                     /gene="fabG"
                     /locus_tag="OO161_000698"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5461867.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(32515..33444)
                     /gene="fabD"
                     /locus_tag="OO161_000699"
     CDS             complement(32515..33444)
                     /gene="fabD"
                     /locus_tag="OO161_000699"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5461868.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(33460..34413)
                     /gene="fabH"
                     /locus_tag="OO161_000700"
     CDS             complement(33460..34413)
                     /gene="fabH"
                     /locus_tag="OO161_000700"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5461869.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLMLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(34481..35551)
                     /gene="plsX"
                     /locus_tag="OO161_000701"
     CDS             complement(34481..35551)
                     /gene="plsX"
                     /locus_tag="OO161_000701"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5461870.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIREASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(35632..35805)
                     /gene="rpmF"
                     /locus_tag="OO161_000702"
     CDS             complement(35632..35805)
                     /gene="rpmF"
                     /locus_tag="OO161_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5461871.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(35857..36378)
                     /gene="yceD"
                     /locus_tag="OO161_000703"
     CDS             complement(35857..36378)
                     /gene="yceD"
                     /locus_tag="OO161_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5461872.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            36577..37161
                     /gene="yceF"
                     /locus_tag="OO161_000704"
     CDS             36577..37161
                     /gene="yceF"
                     /locus_tag="OO161_000704"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5461873.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(37273..38232)
                     /gene="rluC"
                     /locus_tag="OO161_000705"
     CDS             complement(37273..38232)
                     /gene="rluC"
                     /locus_tag="OO161_000705"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5461874.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
BASE COUNT         9466 a        10266 c         9406 g         9223 t
ORIGIN      
        1 tggaagtaag agactttgca ttaatactgg atgagtggct ggataaagac atggtcagca
       61 cagcgagagt aaatcgtgga ttatgggttg atatttataa agaagcacag catgcagggg
      121 aagttcctcc tggatggaat cctccggagg ctacccgtaa accgatccct aaagtaacca
      181 gagccaggct caccatggaa gactggcaaa aaatttacaa tgcaacgcct gaaaaacact
      241 ttatccgtaa cgcaatgctt cttgcgattg ttactggtca gcgccgtgat gacatttgcc
      301 acatgcgttt ttcagatgtg tggaacgaac acttgcatat cacccaggga aaaaccggaa
      361 tgcgtctggc gttaccgctt acactacgct gtgatgccat tgggataacg ttaaaagaag
      421 ttattgatgg gtgccgagac agaatattaa gtccatatct aatccatagt cggcaccaga
      481 aacaaccgaa gccgatgagt aaagacaacc tgagcgacta ctttgccaaa gcacgggatc
      541 tggctgggat aattccacca gcaggaaaaa ctccgccaac atttcatgaa caacgctctc
      601 tatcagaacg gctgtaccgt gcacagggta tcgatacaaa aacattacta ggacataaag
      661 tccaggcaac caccgatcgc tataacgata ctcgaggtca ggaatgggtt aagctggtta
      721 tttgacaaac accatatgga atatccgtca ccatctcagg aaaaaaatgt tgtaaaatgc
      781 gtggcctagt tttggggaaa cgttttggag agattttgga gaaaggaaaa aattgaataa
      841 attcaaaatc ctgaaagtgc tccaccttat gaataagaag gtggaactcg aatgatcggt
      901 cgactgcttc gcggcggttt tatgaccgct atctacgcgt acctgtatat cccaatcatt
      961 attttgattg tgaactcctt taacagctcg cgctttggca tcaactggca gggttttacc
     1021 accaaatggt atagcctgct gatgaacaac gacagcctgt tacaggcagc gcagcattca
     1081 ctaacaatgg cggtgttttc ggcgacgttt gctacgctta tcggttcact gacggcagtt
     1141 gcgctgtacc gttatcgctt tcgcggtaag ccgttcgtta gcggaatgct gtttgtggta
     1201 atgatgtcac cagatatcgt gatggcaatt tcgctgctgg tgctgtttat gctgctgggt
     1261 attcagcttg gcttctggtc gctgctgttc tcgcatatca ccttctgcct gccatttgtg
     1321 gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc ggatgctgga agcggcgaaa
     1381 gatctcggtg ccagcgaatt taccattctg cgaaaaatca tactgccact ggcaatgcca
     1441 gcggtggcag cgggctgggt gttaagcttt accctgtcga tggacgatgt ggtagtttct
     1501 tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa aaatttattc gatggtcaaa
     1561 gtcggcgtat cgccggaagt taacgcgctg gcaaccatat tactggtgct gtcgctggtg
     1621 atggttattg ccagtcagct tattgctcgt gataaaacga aaggtaacac aggggacgtt
     1681 aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc tctggcactg ggcgccgctc
     1741 acgccgatga caacaacacg ctgtatttct acaactggac cgagtacgtg ccgccaggac
     1801 tgcttgaaca gttcaccaaa gaaaccggta ttaaggttat ctattcgact tacgagtcga
     1861 acgaaaccat gtacgccaag ctgaaaacgt acaaagacgg tgcgtatgac ctggtggttc
     1921 cttcaaccta ttacgtcgat aaaatgcgta aagaagggat gatccagaag atcgacaagt
     1981 cgaagttaac gaacttcagc aatctcgatc cagacatgct caacaagcct tttgacccga
     2041 ataacgacta ctccattccg tatatctggg gggcgacggc gattggtgtt aacggtgatg
     2101 cggtggatcc gaaatctgtc accagctggg ccgatctgtg gaagccagag tacaaaggca
     2161 gcctgctgtt gaccgacgat gcccgtgaag tgttccagat ggcgctgcgt aagctgggct
     2221 attccggtaa caccaccgat ccgaaagaga ttgaagctgc atataacgag ctgaaaaaac
     2281 tgatgccaaa cgtcgcagcg tttaactccg ataacccggc taacccgtac atggaaggcg
     2341 aagttaacct cggcatgatc tggaacggtt ctgctttcgt tgcacgccag gcgggtacgc
     2401 ctattgacgt ggtgtggccg aaagaaggcg gcatattctg gatggacagc ctggcgatcc
     2461 cggcaaatgc caaaaacaaa gaaggcgcgc tgaaattgat caacttcctg ctgcgcccgg
     2521 atgtggcaaa acaggttgct gaaactatcg gttatccaac gccaaacctt gcggcgcgta
     2581 agctgttaag tccagaagtg gcgaacgata aaacgctcta cccggatgct gaaaccatta
     2641 aaaatggcga atggcagaat gacgttggcg cagccagcag catttatgaa gagtattatc
     2701 agaagctgaa agcaggacgt taattcagca agcgggcggt ggtattaccg cccgtttttt
     2761 atttagcaag gatgtggcta atgagtcagg acagtagagt tttatcccac tctttcgtcg
     2821 gtattgtggt ccgctcgtag gcctgataag cgtagcgcat caggcaatgc ttcctgcttt
     2881 ataatccctt cagcaacttt tcaacaaact caggcaccac ctggcttgcc gggccgttat
     2941 atttctcggc aaattcatta ccgacctgac ttggttcgag attcagttcc acggtgtgcg
     3001 cgccatgcag tttcgcttcg tgaacaaacc cagccgccgg ataaacatgc ccggaagtac
     3061 caatggcaat gaaaatatca gccatcgaca acgccatata aatttcatcc atgccgagtg
     3121 gcatttcgcc aaaccaaacg acgtgcgggc gcaagggtgc cgggaactgg caacaatggc
     3181 atttatcttc tggcgtaacg tctccggtcc agtcgagaac ctgaccactt tgcgaacaac
     3241 gaactttcag caattcccca tgcatatgaa tgacattggt attacctgcg cgttcatgca
     3301 ggttgtctat attctgcgtt accagcaaaa agcgatcgcc gagagcgtct tgcagtttag
     3361 ccagcgcaag atgcgcggcg ttaggctgaa tttttggctg ctgcagctgt cgacgacggg
     3421 cgttataaaa cgcttgcacc agttcaggat cgcgatcgaa accttccggc gttgccacat
     3481 cttcaacccg atgttcttcc cacaggccat ctgcggcgcg aaaggtacga atacctgatt
     3541 ccgcagaaat tcctgcccct gtcagtacga gtactcttgg tttttccatt gcttccggca
     3601 ccactttatc tctgaaaaaa tacgctgacg caaacgctcg cgcaggcggc gtttattttt
     3661 acgaaaacga cttaaccgat gaccccgacg cgacagcata gcaacctctg ttgtttaatc
     3721 ggttagatgt aggaaggccg caccacgcat tcctcccgca tcaccgtggt gcgcgcgttc
     3781 aatgcgcgga acacgagcta caggtaagag atgacgaggc agcctgtccg ccagttgcgt
     3841 tgtgattgcc gggaaattcg ataagccacc accaatgacg accaggtcag ggtcaacaat
     3901 ggtcaggata tttcccagac aaaccgctaa taaatccaga taacgctcaa cgtgcgccct
     3961 tgcctgctca tcgccttgat tataaagcgc aataatttcg ggagcctgca actgttgatg
     4021 ataatagtgt tgatacagcc acgcaaaacc gcgaccagac agataattct caatgcagcc
     4081 atgctgacca cagccgcagc gacgtaacgg gaaatccaac cccatcatgg ttaacgcatc
     4141 aaccggcaga cgcatatggc caaactcgcc ggtaatgtag cttttaccgg taatcggttt
     4201 gccgttgaaa atcagcccac cgccaacgcc ggtaccaaga atcaacccca tcaccagtgg
     4261 atattgcgta aattcatcat cccaggcttc tgaaagggca aaacagttgg catcgttatc
     4321 aaggcgtaca tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt taccgctggc
     4381 tgcggggaca ttggcggtat acagcgtacc atcttctgtt tccggcattc ccggtatacc
     4441 aatgccgaca gaacctctac agccaaaacg ttgatcagct tcggctacca gctcgcacac
     4501 tgcatctaaa aatgcgtcat agctgtcacg cggtgtcggc acccgctttt cccactgcaa
     4561 ctgccgaccg ctatcaaaca cgccaagcgc aatttttgtt ccaccaatat caaacccgta
     4621 atacattgcc gctccttgtt ttaatgtact gcctttactg gccgctaagg actcgcgcag
     4681 ggtcaatatt actggcgcgc cgcgccggat accaacttgc caaaagactc agcaacaatg
     4741 ctgtgaccag tacgtagaag acgtccagcc agtgcaattc cgatggcagg aagtcaataa
     4801 aatagatatc gctggagagg aactgatgac cgatcagctt ttcaatccgc tcaataatcg
     4861 gggtaagttg cagtgaaaca actacgccga taatcagccc acacaggctg ccgaacagcc
     4921 ctgccagcaa tccataccag acaaagatgg cgcgaattaa accatctttc gcccccagcg
     4981 ttcttaatac tgcgatatcg ccactcttgt ctttcaccgc catcactaag gtggagacga
     5041 tgttgaaaca ggccacgcca atcaccagta ccatcgccag atacataatg gcgcggatca
     5101 tctggatatc gcgatacata tagccgtaag taccaatcca gcttttaata taaacatagc
     5161 tgttggtcat ttcacccgca tcgcgtacca gcttattggc gttgaaaaca tccgtcattt
     5221 taagggcaat acctgacacg ctggaaccca tatcaagata ttgttgggca tccgccagcg
     5281 ggatcatggc aaaactgtga tcgagttggc cactcaactg caaaataccc gcaacgtaca
     5341 aacgcacacg ttttggctgc atcagtttat gctcaggatt cgagttgggg atcatgatcg
     5401 acacccaatc gccctgcttc actttcagcg catccgccac gcctttgccg atgataattt
     5461 gctgttcgcc cgctttaaaa ttgcgccacg catccccctg aacaaacgag ggtaatgcgc
     5521 tcagacgctg ttcctgttgt gggttaacgc ccttcacctg gattgcgcgc agattcgctc
     5581 cactttccac cagcccggtg aaattgatat acggcgcagc ggcggcaata cccggcactt
     5641 tctgcacttt atccagtgct tcctgccagt tagtccatgg ctgatttacc gcttcgattt
     5701 caccatgcgg caccaccgcc agaatgcggt tattcagttc gcgttcaaag ccgttcatcg
     5761 cgcttaagcc gacgatcaat accgccacac cgagggcaat gccaatggta gaaatgacgg
     5821 agatcagcga caccatgccg ccgcgccgcc gtccgcgact aaaacgcagg ccaattaata
     5881 acgataaagg catcgccatt actccgcccc catcaggctc agttccgccg tcagacgtcc
     5941 atcacgcatt tctaactggc ggctcatacg tttcgccagt tgcaggtcgt gagtaaccac
     6001 caggaaggcg gtgccctgca agcgattcaa ttccccaagc aactggaaga tgctgtcggc
     6061 gttacgcgca tcgaggttac cggtaggctc atccgccagt accagacgcg gattattgac
     6121 cagcgcacgg gcaatcgcta cacgctggcg ttcgccgcca gaaagttcag atgggcggtg
     6181 attcgcacga tgctccagcc ccaccgcttt taacatctcc agtgcacggc tgttgatttc
     6241 agcgggcttt ttcttgccaa tcagcagcgg catagccacg ttttccaggg cggtaaaatc
     6301 cggcagcagg tggtgaaact gataaataaa gcccagtttc tggttgcgca gttctgcttt
     6361 tgctgccgac gacagtttgc tcatcggttg accgttaaag atcacatcgc cggaggttgg
     6421 cgtatccagt ccgcccagca ggtgcagcaa ggtgctttta ccggaaccag agctaccgac
     6481 gatcgccatc atttcacctt cgccgacact gaaactgaca ttgtgcagta catcggtttg
     6541 cacactgcct tcctgatagc gtttgcacag gttgtcgcat tgcaacagga tcttattcat
     6601 aacgtaaagc ctcagcgggt tgtgtggcgg cagcgcgcca tgaagggtaa agcgtagaca
     6661 gcagcgcgat agccatcgcc accagcgcaa taacaatgac ctgtaaaggt tcgatagcca
     6721 tcggcagcgc cgcgccatca agcaggacgc cgattattgg catcagatta tttaactggc
     6781 tggcaagcag tgcgccaagc gccgctccga ggatcgcacc gataatcccg gcgctggccc
     6841 cttgcaccat aaagatcatc atgatttgtc gcggagttaa gccttgcgtt tgcaggatcg
     6901 ctacttcgcc ctgcttctcc atcaccatca gccccagcga ggtaataatg ttaaacgccg
     6961 caacggcgac aatcaggctc agcagtaaac ccatcatatt tttttccatg cgtacggcct
     7021 ggaacaactc gcctttacga tcacgccagt cctgccattt gctgtcttca ggcagttttt
     7081 gctgacttaa tgagtcgact ttcagcggct catccagcca caaacgccag ccggtaatat
     7141 tgcctgccgg ataacgcatc agacgcgagg catcctcaat attcaccagc atttcattgc
     7201 catcgacttc actgttggcg gcgaaagtgc caatcacatt gaacaggcgc tgacttggaa
     7261 tacgccccat cggcgtgaac tggctggcag atggtaccat catgcggatt tgatcaccgc
     7321 gattaacgcc tagctgtgag gcaagttgtt cgccgaggat gacattatat ttccccggct
     7381 cgaggtcagt ttgtttcaca ttgaccagat acggcgttag tggatctttt tgcgccggat
     7441 cgatacccag catcaccccg accgccacgc tgcgcgcgct ttgcagtacc acatcaccgg
     7501 tagtaatagg tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc gggagttgct
     7561 gcgggttaag agagccatgc tcagaagaga gaattgcctg tggcatcagg ccaaggatgt
     7621 tgttttgcag ctcgcgctca aagccgttca tcactgacaa tactgtgacc agcgccatca
     7681 ccccgagggt aatgccgatg gtagaaagcc aggagacgaa acgaccgaag cgatccgctg
     7741 cacgcccacg catgtaacgc aggccaataa atagagcgac aggttggtac atgaaatccg
     7801 tctggttgct gtagcaaaga cacgagtata taagcgaaag caagatgggt aaacgtctga
     7861 actgtaagtg atcgtcaggg ctaatctgaa aagcccccgt aaatcaatcg gctaaattgc
     7921 catgttttat catcaatctt gccgcctgat cttaatctga aaaatcatcc aatacagaca
     7981 gatacccatt acacctcggg tcataacccg caggatcaac cgcatgtaaa ttaaaaaaga
     8041 gcgtgttgtc ctgatgaaac aaaaagagct atggattaac cagatcaaag ggttatgtat
     8101 ttatccggtg gtgatttatc actcggtcat taccttttat ccgcatatga ccacttttca
     8161 gcatccgtta tcggaagtcc tgagcaaatg ctggatctat ttcaatcttt accttgcccc
     8221 ctttcgtatg ccggtttttt tctttatatc cggctatttg attcgccgct atatcgacag
     8281 cgtaccgtgg ggaaattgtc tcgataaacg gatctggaac atcttctggg tgctggcgct
     8341 ttggggcgtg gtgcagtggc tggcgttaag tgcgctgaac cagtggctgg cacctgagcg
     8401 cgatttaagc aatgcctcca atgccgctta tgccgattcc accggtgagt tcctgcacgg
     8461 gatgatcacc gccagcacca gcttgtggta tctgtatgcg ttaattgtct atttcgtggt
     8521 atgtaaaatt tttagccgtc tggcgctgcc actattcgcc ttgtttgtac tgctgagtgt
     8581 ggcggttaat ttcgttccca cgccgtggtg gggaatgaac agtgtgatcc gcaatttgcc
     8641 ttattacagc cttggcgcat ggtttggcgc aacaataatg acctgtgtta aagaggtacc
     8701 gttgcgccgc catctgctga tggcttcttt gcttaccgtt ctggcggtcg gtgcctggtt
     8761 gtttactatc tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga agctttttta
     8821 tcagtacgag caacgattcg gtatgcgctc caccagcctg ctgaatgtga ttggctccaa
     8881 caccattgct atctacacca cccatcgcat tctggttgaa atattcagct taactctgct
     8941 tgcgcaaatg aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc tgctggttta
     9001 tccctttgtc agtttgttca tctgtactgt tgcgggcttg ctggtaagaa aactttcaca
     9061 gcgtgcattc agcgatctgt tgttctcccc gccttcactg cccgctgccg tcagttactc
     9121 ccgctaagcc atcgccccag ccggggcgat tcaatttgct aaaccatgtc gattacggat
     9181 aatgaacagc attgcttatc agagatatgc ccccatatgt tgaggcatat cctaacgaga
     9241 atctgacaac cgttatgcct gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc
     9301 agcgtctgct gggcgagtta accggcgcag cctgtgcaac gctggtagcg gaaattgccg
     9361 aacgtcacgc cggtccggtg gtactcattg caccagatat gcaaaatgct ctgcgtttgc
     9421 atgatgaaat cagtcagttc accgatcaga tggtgatgaa tctggcggac tgggaaactc
     9481 ttccctacga cagcttttcg cctcatcagg acattatctc ctcgcgcctt tccacccttt
     9541 accagctacc gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc
     9601 gcgtttgccc acacagtttt ctccacggtc atgcgctggt gatgaaaaaa ggtcagcgtc
     9661 tgtcacgaga tgcattacga acccaactgg acagcgccgg ttatcgccat gttgaccagg
     9721 tgatggagca cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc ccgatgggga
     9781 gtgagctgcc ttatcgtctt gatttctttg atgatgaaat cgacagcctg cgggtgtttg
     9841 acgtcgacag ccagcgcacg ctggaggaag tagaagcgat caatctgctg cccgcgcacg
     9901 aatttccgac cgataaagcg gcaattgaac tgttccgcag ccagtggcgc gataccttcg
     9961 aagtgaagcg cgatccggaa catatttacc agcaagtgag taaaggcaca ttacctgccg
    10021 ggatcgagta ctggcagcca ttgttcttca gcgaaccact gccgccgctg ttcagttatt
    10081 tccctgccaa taccttgctc gtgaatactg gcgatctgga aaccagtgcc gaacgtttcc
    10141 aggctgacac gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc ccgctgttgc
    10201 caccacaatc gctctggctg cgggtggacg agctcttctc agagctgaaa aactggcccc
    10261 gggtgcagct aaaaactaaa catttaccga caaaagccgc gaatgccaat ttaggtttcc
    10321 agaaactgcc agacctggcc gttcaggcgc aacaaaaagc gccgctggat gcgctgcgta
    10381 agttcctcga gactttcgac ggtccggtgg tgttctcggt agaaagtgaa ggtcgccgtg
    10441 aagcgctggg tgaactgctc gcacgaatta aaattgctcc gcaacgcatt atgcgtcttg
    10501 atgaagccag cgaccgtggg cgttatctga tgattggcgc tgccgaacat ggttttgtcg
    10561 ataaggtgcg taatctggcg ctgatctgcg aaagcgatct gctcggtgaa cgtgttgccc
    10621 gccgtcgtca ggattctcgc cgcaccatca accccgatac actgatccgt aaccttgcgg
    10681 agctgcatat tggtcagccg gtggtccatc tggagcacgg tgtcgggcgc tatgccggaa
    10741 tgaccacgct cgaagcgggc ggcattactg gcgagtattt gatgctcacc tatgccaacg
    10801 acgccaaact gtatgttccg gtgtcgtcac tgcatctgat tagccgttac gcaggtggcg
    10861 cggaagaaaa cgccccgctg cataaacttg gcggcgatgc gtggtcacgc gcgcggcaga
    10921 aagcggcgga aaaagtgcgt gatgtggcgg cggaattgct ggatatctac gcgcaacgcg
    10981 ccgccaaaga gggcttcgcg tttaaacacg atcgtgagca gtatcagttg ttctgcgaca
    11041 gcttcccgtt tgaaaccacg ccggatcagg cgcaggccat taatgcggta cttagcgaca
    11101 tgtgtcagcc gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag
    11161 aagtggcgat gcgcgccgct ttcctggcag tagataacca caagcaggtg gcggtgttgg
    11221 tgcctaccac ccttctcgcg cagcagcatt acgacaactt ccgcgaccgt ttcgccaact
    11281 ggccggtacg tatcgaaatg atctcccgtt tccgcagcgc caaagagcag acgcaaatcc
    11341 ttgcggaagt ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa ctgctgcaaa
    11401 gtgacgtcaa gtttaaagat ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg
    11461 tgcgtcataa agagcgcatt aaagcgatgc gcgcgaacgt ggatattctg acgcttactg
    11521 caacgccgat cccacgcacg ctgaatatgg caatgagcgg aatgcgtgac ctgtcgatta
    11581 tcgccacgcc gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag tatgacagcc
    11641 tggtggtccg ggaggcgatc ctgcgtgaaa ttttgcgcgg ggggcaggtt tattatctct
    11701 acaatgatgt ggaaaacatc cagaaagctg ccgaacggct ggcagaactg gtgccagaag
    11761 cgcggatcgc catcggtcac gggcagatgc gcgagcgcga actggaacgg gtgatgaatg
    11821 atttccatca tcaacgtttc aacgtgctgg tttgtacaac cattatcgaa accgggatcg
    11881 acatcccgac agccaacacc attatcattg aacgcgcgga tcacttcggt ctggcgcagc
    11941 tgcaccagtt acgcggtcgc gtcggacgtt cacatcatca ggcatatgca tggctgctga
    12001 cgccgcatcc aaaagcgatg actaccgatg cacaaaaacg tcttgaagcg attgcctcgc
    12061 tggaagatct cggtgcaggc tttgcgctgg caacgcacga tctggagatc cgcggcgcgg
    12121 gtgaactgct tggcgaagaa caaagtggct caatggaaac catcggtttc tcgctgtata
    12181 tggagttgct ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg tcgctggaag
    12241 atctcaccag ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt
    12301 tcattcctga cgtgaatacg cgtttgtcgt tctataaacg tattgccagc gccaaaacgg
    12361 aaaacgaact ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg ctgccggatc
    12421 cggcgcgtac cctgctggat gttgcccgtc tgcgccagca agcgcagaaa ctggggatca
    12481 ggaagctgga aggtaatgag aaaggcgggg tgatcgaatt tgccgagaag aatcacgtta
    12541 atccggcctg gttgattggt ttgctgcaaa aacagccgca gcattaccgc cttgatggtc
    12601 cgacgcgcct gaaatttatt caggatttga gtgagcggaa aacgcgtatc gaatgggtac
    12661 gccagtttat gcgtgaactg gaagagaacg cgatcgctta atggtaaatc gggggcgttt
    12721 ctgcgccccc atcggcaaca tttacaaatt ctttgcactt ccctgcacta tccgacactg
    12781 tcaccatcca taattcaggc ttatctgttt attacaataa ccttatattt attatggatt
    12841 tttggtgatg atcaaaacgc gtttttctcg ctggctaacg ttttttacgt tcgccgctgc
    12901 cgtggcgctg gcgctaccgg caaaagccaa cacttggccg ctgccgccag cgggcagtcg
    12961 tctggttggc gaaaacaaat ttcatgtggt ggaaaatgac ggtggttctc tggaagccat
    13021 cgccaaaaaa tataacgtcg gctttctcgc tctgttacag gctaaccccg gcgttgatcc
    13081 ttacgtaccg cgcgcgggca gcgtgttaac gatcccgttg caaaccctac ttccagacgc
    13141 gccgcgcgaa ggcattgtga tcaacattgc ggagctgcgt ctctattact acccgccggg
    13201 taaaaattcg gtaaccgtgt atccaatagg tattggtcag ttaggtggtg acacgctgac
    13261 accgacaatg gtgaccaccg tttcagacaa acggcaaacc caacctggac gccaacggca
    13321 aacacccgcg cccgttataa agcacaggga attgagttgc ctgcggtagt gccggctgga
    13381 ccggataacc caatgggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg
    13441 cttcatggta cgaacgccga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt
    13501 ctgcgggatg acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat
    13561 atcatcaaca ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta
    13621 catcagccgc tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat
    13681 agcgcaatgc aatcatttaa agatgcagca caaactgacg ctgaagtgat gcaacatgtg
    13741 atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact
    13801 ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga aaaaatcagc
    13861 agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg
    13921 tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt
    13981 gcgccagctg ctcttccagc gatcccagat ttgtccccgc tttagcactg attgtaccga
    14041 ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagctg gcaaatgaca
    14101 tggagcttaa aatcgccgca gcgatgagga tttttacgtt tttcataata gtggccttat
    14161 gcagatgaat gacgtcgcaa gcaattactt aacgatcgat aactaaatga catatgtgat
    14221 ctggatcaca tacaagatat tttttataac aatcattaat taatttaaaa atctttaaat
    14281 ttcagttaaa tagaaagcaa ttatttttat gggatctgga ctggtgaacg gaggtgatta
    14341 tttttataat aacctttcat tcacagtcac caggtacaac ggcatcatgg caactgactc
    14401 aacacaatgt gtaaaaaaaa gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct
    14461 tgataaggcc atgaaattgt tctggcaaca cggttatgaa gcgacttctc ttgcggacct
    14521 cgtcgaagcg accggagcca aagcgcccac gctatacgcg gaatttacca acaaagaggg
    14581 gttatttcgt gccgttctcg accgctatat cgatcgtttt gccgctaagc atgaagcaca
    14641 gctgttttgt gaagagaaaa gcgtgaagtc tgcgctggct gactattttg ctgccatcgc
    14701 caactgcttt accagcaaag acaccccggc aggctgcttc atgatcaaca actgcaccac
    14761 cctctcccca gattcaggag atatcgccaa tacgttgaaa tcacgccatg cgatgcaaga
    14821 gcgcactttg cagcagtttt tatgtcaacg acaagcgcgc ggggaaatcc cgacccactg
    14881 tgacgtgaca catctggcag aattccttaa ttgtattatt caggggatgt cgatcagcgc
    14941 acgcgaaggt gcatcgctgg aaaaactaat gcagattgcc ggaacgactt tgcgtttatg
    15001 gcccgaactg gtgaaataag tcgcaaaaaa agcccctcag cctgagcgaa tgaggggcca
    15061 aattgcagag tacagccttg ttatactttg ttattcaaaa tcagttgccc attactatct
    15121 agcgggatct gcgtacccgg atcgcggtcc atgcggattt tgccctgctg atcgccaatc
    15181 ttataggtca catcataacc gagcattttt tctgacttgt catacaccgt tttacaacgc
    15241 tgttgcgtag tcgtgtaagt atcgctttcc tggagagagc cctggatctg gttaccggca
    15301 tatccaccac ccagcgcccc cacaacagtg gcgacatctt taccgcgacc accgccaaac
    15361 tgatgcccta tcacgccgcc agcaacagcg ccgagcaccg acccggtaat gcgattttca
    15421 tcctgcaccg gtcgacgatg ggtcactgtg acgttgcgac actcctgacg cggtgtttta
    15481 accgtttcct tgattggggt tgcagaaaca acctgagcgt attgcgggcc acgttcaaac
    15541 acgttcagac tggccactgc cgctacgccc agcgcagctg cgacaccaat cccgataccc
    15601 gccaacattg atttattcac gggaacacct ccttctttgc agttatgcca atcatgcaag
    15661 cgaaggagaa aagcagctat cagacagaag atgaaaagcg cgggaatggc tggaaatact
    15721 taatgattcg gaggagtctg aaggaatgcc tgatgcgacg cttaacgcgt cttatcaggc
    15781 ctaccgctcg taccatttgt ccagggcgga tgaggcgttt atgccacatc cgccagtgta
    15841 cgtcgattaa tgcaacttca aacgcggacg gataacgcgg ttaatactcc ccaccagcat
    15901 cattaatccg gttttaaagt aaccatgcag cgcaatctga tgcattcggt atagcgagat
    15961 atatacaaag cgcgcaattc gtccttcaat catcattgag ccgcgcgtca agttacccat
    16021 caggctacca acggtggaga agttcgacag cgataccagt gaaccatgat ctttatactg
    16081 ataatttttc agcggcttac cgtttatctg cgccagaata ttgttcattg cgcaagtcgc
    16141 catctggtgt gcagcctgag cacgcggcgg aacaaagccc ccttccggac gcgtgcatga
    16201 cgcgcagtcg ccaatagcgt aaatgtctgg atcgcgggtg gtttgcagcg tcggttccac
    16261 caccagctgg ttgatacggt tagtttcaag accaccgata tctttcagga agtctggcgc
    16321 tttgatcccg gctgcccaca ccatcagatc agcctcaata tattcaccat ctttagtgtg
    16381 caggccgcct tcatcagcac tggtgaccat ggtttgcgtc agcacgcgaa cgccaagttt
    16441 cgttaactca ctatgggccg cagcagagat gcgtggcggt aatgcaggca aaatacgttc
    16501 tcccgcttct actagcgtta cgttcagggc ttcgttggtc agacctttat aaccgtagct
    16561 gtgcagttgc ttgaccgcgt tatgcaactc agcggagagt tctacacccg tcgcgccgcc
    16621 accgacaatc gcaatgttca ctttgccatt cgcgcccagg ttggcggagt atttcaggaa
    16681 cagattcagc atctcctggt ggaagcgacg cgcctggtgc gggttatcga ggaaaatgca
    16741 gttctctttg acgcctggcg tattgaaatc gttagaggtg ctacccagcg ccatgaccag
    16801 ggtgtcatag gcgattttac gttccggaac cagcagttca cctttctcgt cgcgtagttc
    16861 tgcaatagtg attgttttcg cttcacgatc aatatcaatg acggaaccca actggaactg
    16921 gaaaccatga ttgcgcgcat gggccagata gctcaacgca tcgacgcctt catcaagcga
    16981 gccagtcgcc acttcgtgca gcagcggttt ccacaggtgg ctgtggttac gatcgaccag
    17041 cgtaattttg gcttttttct tacgtcccag cttatgcccc agctgtgttg ccatttccag
    17101 cccaccagca ccgccgccga caatcacaat ctttttcaat ggcgtagtca acgtgacccc
    17161 cttaaattta ttaaccaatt gttaattaat ggttattaac atagctctta attaacaaca
    17221 ggttacagca atgaaaatgt tcttcgctat tgagaataac atgaatggtg cattggtcat
    17281 accaaaattg atgttaatca agttttgttg ctgaaaagat aggcgacttt accgggtgag
    17341 atcaaaaaat agagtttatt gtttattatt tgagcgaatt tgtgagagtg atatagagtg
    17401 aggggtgtat gcagggcgtt cggtaggcct gataagaggc gcatcaggca ttgatgctcg
    17461 actgtcggat gcggcgtaaa cgccttatcc gacttacaga attcggagcg catcaggtac
    17521 cggcaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg
    17581 ctgcaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc
    17641 gtaataatga tgcaactctt cagatgtccg ctggctgtta agcgcttcat cattacgcga
    17701 caaaatcacc aggcaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc
    17761 aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa
    17821 attagggttg aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta
    17881 tccgcccaga ccaacgccgc aaatcagcgg acgctcgtcg acgttcagtt gcaacatttt
    17941 gtccacttct ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat
    18001 caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact
    18061 gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt
    18121 gacctgcttt ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgctcgcct
    18181 tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg
    18241 taacacgctc tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat
    18301 tattgcagac caggatcata tcgcaacctg cgtccagtga tgcctgcccg cgttcggcat
    18361 aactgcccat aatcgcggca ccttccatcg ataaatcgtc agagaaaatt acaccgtcaa
    18421 aacacagttc ctgacgcaaa acggttttca gccagtagga agaaccgctc gccggacgcg
    18481 gatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga
    18541 ttaacgtgct gaaaaccgac atatctttgg cacgaatttc cgcttgcggg cgtgggtcgc
    18601 acggcgtctc tttgtgtgaa tctgcagtta ctgcaccgtg tcctgggaag tgtttcccgg
    18661 tcgttttcat tccggcttca tgcatcccat caataaaccg actggcgatt gctagggctt
    18721 tttctgggtc ggcatgataa gaacgctcgc caatcgccgc gctgatatgt cctacatcca
    18781 gcactggcgc aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac
    18841 ccgcctcttg cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg
    18901 ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta
    18961 ccgccaccac cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact
    19021 gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca
    19081 gtatttcacg ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca
    19141 cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata
    19201 aattgttgat cgccggtttg tcgccagcgg tactcaaacc accctgcttt gagcatcagc
    19261 agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga
    19321 gtggcatagt cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc
    19381 agttccagcg cggtatcacc atctccggca tactcccagt cgatgagttt taaccctgat
    19441 gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt
    19501 cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc
    19561 gccggatcgc tttgctgcca gtacagttcc agtaacggca acagcgttat tcgccagcca
    19621 aaacgtggtt gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa
    19681 tacgttttta ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc
    19741 ggcttcggtg caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg
    19801 gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca
    19861 atgagaaaac taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg
    19921 aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tactgcgaaa cggcaccttt
    19981 acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc
    20041 gttaacgttg ccagaggcgc tggagtacag cacgtaatga gcgccgacat tgcgggcaat
    20101 gcctatggct ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt
    20161 agccatcgac agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt
    20221 tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct
    20281 atcaaccagc aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat
    20341 cggctgcatt gcgccattcc agtcataata gcgaatatgc ggcgcaggcg gtgccgttcg
    20401 atcttcgtgc tcaattgggc ctggttgctg cgggatcgtc ggcaccgagg gcactgtggg
    20461 gacaggctgt tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc
    20521 aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta
    20581 gcgactcatt tttgtcatca agattcaccc cttacaaata tagataaagt ctgactttgt
    20641 gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatggtaa
    20701 cgctgcgtgg cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac
    20761 gataatgaac ggtaacgggt ttttgccttt cgttataaag cgttgaggat gctgaaggtt
    20821 gaatatcaga cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg
    20881 actccatcac cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca
    20941 ccagcagtaa caacaccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc
    21001 tttatgcgcc agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta
    21061 aacctcttgt ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat
    21121 accttcttgc tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg
    21181 ctcagctgag acgtcgttca cagtcggaat gaggatattc ggaatgatca gaatatgcgt
    21241 tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc
    21301 ggaggggatc tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc
    21361 ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtataaagt
    21421 atggtcgagt taaacctgct ctttcaacct taactcacga tttcttaagc aaaaaatcgt
    21481 tttgctgccg tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca
    21541 ttctctgttt ggtttacacg acaaatatga atgcgtatat ttctcatttg catttacaaa
    21601 caaaattatt cgcacactaa atataatcgc tcatcaccct cctccggcat gagcctgacg
    21661 acaacataaa ccatgagatt tcaaatgctt tcaacacaat ttaacaggga taatcaatat
    21721 caagccatca ccaaaccgtc actacttgcc ggttgcatag cactggcact attaccttct
    21781 gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc agccacagct
    21841 ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac caaaatgcag
    21901 atgactcaac gtgatattcc tcagtcggtc actattgtta gccagcagcg gatggaagat
    21961 cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag caaaagtcag
    22021 gcggattccg atcgtgctct ttattattcc cgcggattcc agatcgataa ctatatggtt
    22081 gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgatgcact ttctgatatg
    22141 gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac cgggacgggt
    22201 aatccatctg cggcaattga tatggttcga aaacacgcga ccagtcgtga atttaaaggc
    22261 gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga tttacaaagc
    22321 ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca gaataacgac
    22381 tcatggctgg accgctacaa cagtgaaaag accttcttct ccggcattgt cgatgctgat
    22441 ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga tgttaatagc
    22501 cctacctggg gcggtttacc gcgctggaat actgatggca gcagcaacag ttacgatcgt
    22561 gcacgcagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa ggtctttatg
    22621 accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac ccactctgaa
    22681 gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc ggatggtatg
    22741 ctggttgggc catacagtaa ttgtggacct ggctttgatt atgtcggcgg caccggttgg
    22801 aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag ttatgaattg
    22861 tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa caatcgttac
    22921 ttcagttcat gggccaacat cttcccggat gaaattggca gtttctacaa ctttaatggc
    22981 aatttcccac aaaccgactg gtcaccacag agcctggcac aggacgatac cacacatatg
    23041 aaatcgttat atgctgccac tcgagtcacc cttgccgatc cgctgcatct gatcctcggc
    23101 gcacgttata ccaactggcg ggttgatacg ctgacttaca gcatggagaa aaaccacacc
    23161 acgccttacg ctggtctggt gtttgacatc aatgacaact ggtcgaccta cgccagctat
    23221 acctctattt tccagccgca aaatgatcgt gacagttcag gcaaatatct ggctccaatc
    23281 accggtaaca actacgagct gggtctgaaa tcggactgga tgaatagccg tctgaccacc
    23341 acgttagcca tcttccgtat tgagcaggat aatgtcgctc agtccaccgg tacacctatc
    23401 cccggcagca acggcgcaat taccgacaac tggcagctga catttggcgc aacgcgctat
    23461 attgcagagg ataacgaagg aaacgccgtt aatcctaatc tgccacgcac cacggttaaa
    23521 atgttcacca gctatcggtt gcctgtcatg cagagttgac agtcggcggt ggtgttaact
    23581 ggcaaaatcg cgtgtatacc gacaccgtga caccgtatgg caccttccgc gccgagcaag
    23641 gtagctacgc gctggtggat ctcttcaccc gctaccaggt gacgaaaaac ttctcgttac
    23701 aggggaacgt caataacctg ttcgacaaaa cctacgatac caacatggaa ggttctatcg
    23761 tctacggcgc accgcgtaat ttcagcatta ccggcacgta tcaattctga ataacacctg
    23821 taaaaaaggc agccatctgg ctgcctttgt ctccccaacg tcttacggat tagtggttac
    23881 ggatgtactc atccatctcg gttttcaggt tatcggattt agtaccgaaa atcgcctgaa
    23941 caccagaacc cgcaaccact acgcccgctg cgcccagttt cttcaggcca gcctgatcca
    24001 ctttagacac atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg aggttagtaa
    24061 tgttttcttt accaccaaat gcagcaacca gagccggtgc catttcgctg gtacctgttg
    24121 cttttgcatc ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt gctttaatca
    24181 gcacgcggaa gatggtgtag taaacaatcg cataaccgat accgacgatc gggaacagcc
    24241 acagtttgct gctgttacca gacagaacga taaagtcgat cagaccgtgc gagaacgacg
    24301 taccgtcacg catccccaga agaatacaga ttgggaatgc caggcctacc agaatcgcgt
    24361 ggatgatgta caggatcggc gcaacgaaca tgaaggagaa ctcgatcggc tcggtgatac
    24421 cggtcaggaa cgaggtcagc gccgcggaga tcataatacc gcccactttc gcgcggtttt
    24481 ctggtttagc agagtgccag atagcaattg cggcagctgg cagaccgtac attttgaaca
    24541 ggaagccacc agacagttta cccgcagtcg ggtcacccgc catataacgc ggaatgtcgc
    24601 cgtggaaaac ctgacctgct gcgttggtgt attcaccaat ctgcatctgg aaaggtacgt
    24661 tccagatgtg gtgcagacca aacggtacca ggcaacgttc gatgaaaccg taaatgccaa
    24721 acgcaactac cgggttctgg taagcagccc actgagagaa ggtctggatt gcagaaccaa
    24781 tcggcggcca aatgaaggac agcacaacgc cagtaaagat ggcagccagg ccagaaatga
    24841 tcggcacaaa gcgtttaccg gcaaagaagc caagatactc aggcagctta atacggtaga
    24901 aacggttaaa catgtacgct gcgatcgcac cggagataat ccctccgagt acgccagtat
    24961 ccgccaggtg tttagaggcg atttcttcag caggtaaatg cagtaccagt ggcgcaacca
    25021 cggccatggt tttaaccatg atgccatagg caacaactgc ggccagcgcg gatacgccat
    25081 cgttattggt aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg tttgcaaaga
    25141 cggaaccgcc tgcttctgcc ataacatgcg atacaacggc gggcagccag ctgaaattcg
    25201 cggaaccgac gcccagcaga atacctgcga taggtagtac ggataccggc agcatcagcg
    25261 atttaccgac cttttgcagg ttagcaaatg cattcttaaa cataattgag agtgctcctg
    25321 agtatgggtg ctttttttac gttctcacgc gtggcaaggg gggagagcct cgccgtgtac
    25381 agggcatcta agcgcccttt atttattaca cagagtaaaa taattcagtg ccaatttgtt
    25441 tgacggctat cacgtttcaa cttcagatca ttggagaagt tgcacatttt gacaaaacat
    25501 atgtcacaat atttcaaacg cggttattca ccgcccaatt ttcggcggtg aactttactc
    25561 gttttagcca ttaattacga gctttaaaaa aactcattca acggatggat tgaaggcggg
    25621 aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc agttcttcaa
    25681 cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc gcaggttgat
    25741 tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc accagtaatc
    25801 gatccagggg gacataacgc gcagcatcgc gcagttgctc cgcattacgg aaggtcacaa
    25861 tgccggaaaa ggagatgtaa aatccgagat ccagtaattt acccgccgtt tctctgtcct
    25921 ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc agaattgcca
    25981 gcgtatcggc acgggcgtca cgggtatgga cgataaccgg cttgttcagt tcacgaccaa
    26041 tctggatatg atggatgaag gactcttgct gacgtacttt agtttccggt gtgtaataat
    26101 aatccagccc ggtttcaccc agcgctacaa caccctcttc tgccgccaga cggcgtaaat
    26161 cttctacatc gtagggatca ttctggttga gtggatgcac gccacaagaa aatacgacgt
    26221 tgtcacgttc gcctaccaga tcccgcatat gtaaataacc cggtaatgtt gtggcgactg
    26281 ccagacaaaa tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc acgtccttat
    26341 gcaaagattc ataatccaga ccatcgagat ggcagtgtga gtcgactaaa aacatgatgt
    26401 ctctcttaaa gatgaggaac cggtagcaca acgcccggtt gcaggtaatg ctcaatacgc
    26461 agtaaaagat cggtgatgag aagctcgcgg ttgatgcctg tgacagacat taactgttca
    26521 cgaatgtggc aaacatcccc cagtatagcc tgcaggcgcg agggagaaag atggtttgcc
    26581 agttcggcga ccaggcccgg cacatcaaca ttggtcacct gtgcagcgcc atgatggcgt
    26641 tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc ttgttcatga
    26701 ttaagggccg ccagcagcga ataccagtcg cccgatggta cgctatatgc caacgcctga
    26761 cacaatgttt cacgagcctg ccagttatct ccctgaaaca acgccagtgc cgcgccaggc
    26821 gaaccggcac ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt cacttcgcgt
    26881 gaaagccagg tcacggcgta ctgttccggc ggcggcgcaa ggtaatgtaa ccgacaacga
    26941 ctacgtaatg ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa ccaggtttct
    27001 gctggtggct cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt cagtaaggca
    27061 gcatcggtta cccagacaac tttcgcacca cctaggcgtg cgtgctcatt cagcttttcg
    27121 gtgacctcac gtaccgcatc aatgcccagc gtatttttcc ctttttcggg agccagggtg
    27181 tagtaatcgg gatgcgtgcc agcctgcatt aactgacatc cacgacagtg accgcaactt
    27241 ttgtggccct gcggttgttg gcagagtaaa taacggctca gggcgtagat taaagcatca
    27301 tcgcccatgc ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc ggcctgatag
    27361 ctggctacca gtttttcgaa atcaggtcgt aaccatggat accatctcat gcgtccaact
    27421 ccttcaccca gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc agcggctggg
    27481 tggcatcaat ggtatgaata cttttatctt gtgcagccag ttccagatag cgggcgcggg
    27541 tgcgattaaa gaaatcgaaa gactcttgct caatacgatc cagctcgccg cgcgcacgcg
    27601 cgcgttttaa gccaacttcc ggggtaacat cgagatagag cgttaagtcg gggcgaaaat
    27661 ccccgagaac agcatcacgc agtgttgcca gcatatgttg gtcaatacca cgtcctccgc
    27721 cctgatacgc ctgagtggag agatcgtggc gatcgccaat cacccaggtg ccgttagcca
    27781 gagctggttt gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac atcagaactt
    27841 cggctttatc ggtaatgact tcatcgccta ccgatttgat atccagcacc aggcttctta
    27901 acttttcggc aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg cggataccca
    27961 gttgctcaag cgtctcaacc accacattac gcgcggtagt tttgcctgcg ccttccagcc
    28021 cctcaatgac gatatactta ctgcgcattt ttttccttaa gcactttcag ataatcctgc
    28081 acagacttgt tatggctggc aagattggta ttaaacgtgt gaccaccttt accatcggcc
    28141 acaaaataga gatacggcgt ttttgccgga tgcgcagcag ccttcagcga atccgccccc
    28201 ggcgtagcta tcgcacctgg cggcaaaccg gtaatggtat aggtgttata cgctgtcggc
    28261 gtttccaggt ctgcacgaga aagtttgcca ttataacgct ctcccatccc gtaaatcacg
    28321 gttgggtcgg tctgcaggcg cataccaatg cgtaaacggt tgataaatac cgaggcaacc
    28381 tgatcgcgtt cactggcaac ggcggtttct ttttcgataa ttgatgccat cgtcaccagc
    28441 tggtttttat ctttataagg cagaccgtcc gcacgccctt cccaggcgct atcgaccgct
    28501 ttcaccattt tcttgtgcgc tcgcttgagt aacgcgacat cggtggtatt ggcggtatac
    28561 atccaggtgt ctggccagaa ccaaccttca atccactccg ggttttccag ttcaagttcc
    28621 tgcgctacgg tggcgtactt atcgtcactc agcgtgtgct tgatatacgg agcctcacgc
    28681 aattgcttga ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg gaactgtgct
    28741 tctttaccgc tttccagcaa tttcagcatc tcgcgcacgg tcatctgcgg tgtaaagcgg
    28801 taagtcccgg ctttaaagtg agaaagatcc ggttcgatac gcagcaacca ttgaaaaacc
    28861 cgtgggcgat tgatgatctt atcggcataa agctgttcac caagcgccag acgtccggtc
    28921 cctggcttca gggtaaatat cgtctcttct ttgataagca atttgctgtc ggcaagatgg
    28981 cgaaccttcc agacgcccac accagcggcg atacccagta ccaccagcaa taacaagata
    29041 atcaataaca cttttttcat gactaattcg ggcgctcaca aagtggggct aaatattcat
    29101 ataacgttgc tgacgaaaag gagacatcgc cacaggcacg tacgggcatc actggcatta
    29161 acgcattaca aataaccatc tcatctgcct gcaacgcctc ttccagagag gcttgcactt
    29221 cgacaagctg ataagaggat tgtgccagca aacggataca gaattgtcgc ataatgccgt
    29281 taacacctgc ctgatccagt cgcggcgtat aaactacatt gcccttccgc cagaacaaat
    29341 tagccgcaca gcattccgta acccaccctt cgctgtcaag gaccagcgcc tcatcagcgt
    29401 ttgtctgctc aagatgagag cggatcaata cttgctcaag ccgattaaga tgtttaatac
    29461 ctgcaagatg gggattgcgc cccagccgca ccgggcttag cgccaacgtc atcccttcgt
    29521 tacgcaaacg gtcgtaatgt gcaggataaa ccgtaacgga gagaatccgc gttgccggtc
    29581 cactgttcaa tgtgctgtac cctcgcccgc cactaccgcg actgatcacg actttcagca
    29641 caccattttg ctgttctgct gccagcgttt tcatctcttg ttcaagctga ggccagaagt
    29701 cacagaaaat catcaaccgc tgacaagcat cctgtagtcg ctggatatgc gccgataaca
    29761 aactgacttt accgtcgata actctggcgg tggtaaaaca accatcacca aactgcgttg
    29821 cccgatcgct tactgccagc gattcctgct tatgaccgtt aattaagaac ataccggctc
    29881 cttatcagag aattgttagt gtggcagcat gttcactacg gaacaagtcg gaataaaagc
    29941 taagaaaaaa ggcccgcaag cggacctttt atatgggtgg gaaatgacaa cttagatctt
    30001 tttaaagatc aaagaaccat tagtgccacc gaagccgaag gagttacaca gcgtgtattc
    30061 cattccgctg acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac cttcatccag
    30121 gttatccagg ttgatggttg gcggaacagc ctgatcgcgc agcgccagga tggagtagat
    30181 agactctact gcccccgccg cacctaacag gtgaccggtc atagatttcg tggagcttac
    30241 caacacacgg cttgcagctt caccgaagat ggctttcacc gcctgcgctt cagctttatc
    30301 gccagccggc gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg cttcaatgcc
    30361 tgcatcacgc agagcatttg ccattgccag agctgcgcct gcgccatttt ctggcggtga
    30421 cgtcatatga taagcatcgc tgctcatacc aaagccgacg agttcagcgt aaattttcgc
    30481 accgcgtttt ttcgcgtgtt cgtactcttc tagtaccagc ataccggcac catcgcccag
    30541 tacgaagcca tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt tatcattgcg
    30601 ggtagataat gcacgtgccg cgccaaaacc accaacgccc agcggcgtac tggctttctc
    30661 tgcgccacct gcaaccatca cgtcagcatc gccatacgcg ataatacgcg cagcatgacc
    30721 aatgttgtgc acgccggaag tacaggcggt cgcgatagag atgcttgggc cacgcaggcc
    30781 atacatgata gtcagatgac ctgccaccat gttcacaatc gttgacggaa cgaagaatgg
    30841 gctgatctta cgtggaccac cgttcatcag agatgtgtgg ttttcttcga tcagtccgag
    30901 gccgccaatc ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt ccgttatttc
    30961 aaggccagaa tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa tgaaggcatc
    31021 catcttgcgc tgttctttgc gcgagataat gtcctcacag ttaaaatcct ttactaagcc
    31081 agcaaatttc gttgcatagg cgctagtatc gaaatggtcg attaggctga tgccactctg
    31141 accggcaagc agagctttcc aggtagactc tacggtattg ccgacaggag acaacatgcc
    31201 cagtccggtc acaactacac gacgcttaga cacgtttgtc ctccagggag ggaaaaaatg
    31261 attctagtgg gacaaaaaga taaaactcag gcggtcgaac gaccgcctgg agatgttcac
    31321 ttacgcctgg tggccgttga tgtaatcaat ggcagcctga acggtggtga ttttctcagc
    31381 ttcttcgtcc ggaatctcag tatcaaactc ttcttccaga gccattacca gctcaacggt
    31441 gtcaagagaa tccgcgccta ggtcttcaac gaaagaagca ttgttggtaa cttcttcctg
    31501 cttaacgccc agctgttcgc cgataatttt cttaacgcgt tcttcgatag tgctcatact
    31561 cttaaatttc ctatcaaaac tcgctttcgc gatggttttc gtagtgtata aaatgttgaa
    31621 aaatttgcaa ctaaatcccg gcaggtctta ccacgatttt acgttatttt gaggccttac
    31681 cccccaataa cgcaaatcat tttgcactaa ttattactca taaccacgca gactgcgcgc
    31741 aacatgagca ttttgtgcaa atcgcggtca gaccatgtac atcccgccgt tcacatgcaa
    31801 agtttcaccc gtgatgtaag ctgcttcgtc ggatgccagg aatgcaaccg cgttggcgat
    31861 ttcctgtgcg ccgccgaggc gacccgcagg aacctgcgcc aggatacccg cacgctggtc
    31921 atcgctcagc gcacgtgtca tgtccgtttc aataaagccc ggagcaacaa cgtttacagt
    31981 aataccgcgt gacgcaactt cgcgcgccag tgatttactg aagccgatca agcccgcttt
    32041 cgccgcagcg tagttggcct gaccgccatt tcccatggta ccaaccacag aaccgatagt
    32101 gataatacga ccatgacgct ttttcatcat agcgcgcatt accgcttttg acagacggaa
    32161 aacagatgaa aggttggttt cgataatatc gttccactct tcatctttca ttcgcattaa
    32221 caggttatca cgagtgatac cggcattatt gaccaggata tccacttcac caaattctgc
    32281 gcgaattttt tccagaacag attcgataga tgccgggtcg gtcacattca acatcagacc
    32341 tttgccgtta gcacctaaat aatcactgat cgcctgagcg ccattttcac tggtcgcagt
    32401 gccaataact ttcgcgccac gggctgcgag cgtttcagca attgcgcggc caattccgcg
    32461 gcttgcaccg gttaccagtg cgatttttcc ttcaaaattc atgattttcc tcttttaaag
    32521 ctcgagcgcc gctgccatcg ctgaaggttc gttcagcgcc gaggcggtca gggtgtcgac
    32581 aatgcgtttc gtcaggccag ttagcacttt gcccgggccg acttcataga gatgttctac
    32641 gccttgcgct gccatgtact caacagactt cgtccactga accgggttat acaactgacg
    32701 taccagtgcg tcacgaatgg catcaccatt ggtttcgcat ttcacatcaa cgttattcac
    32761 aacaggaact gttggtgcgt taaaggtgat tttcgctaat tctactgcca gtttgtcggc
    32821 tgctggtttc atcagcgcac agtgagacgg tacgctcact ggtaacggca gcgcgcgttt
    32881 tgcgcccgca gctttacagg cagcgccagc acgctcaacc gcctctttat gaccggcaat
    32941 aaccacctgt cccggagagt taaagtttac cggagaaacg acctgacctt ctgcagcttc
    33001 ttcacacgct ttcgcaatag acgcatcatc cagaccgatg attgccgcca tagcgcccgt
    33061 gccttctggt acggcttctt gcatgaactt gccgcgcatc tcaaccagac gcaccgcatc
    33121 agcgaaatca atcacgccag cgcaaaccaa cgcggagtat tcccccaggc tgtgaccggc
    33181 catcattgcc ggtgctttac cgccctgctg ctgccatacg cgatacagcg caacagatgc
    33241 agtcaacagc gctggctggg tttgccaggt tttattcagt tcttcagctg gcccctgctg
    33301 ggtcagcgcc cacaggtcgt agcccagcgc cgcagaagct tcagcaaacg tttcttcgac
    33361 aattggatag ctcgccgcca tatcagccag cattccaacg gtttgagaac cctgtccagg
    33421 gaacacaaat gcaaattgcg tcatgtttta atccttatcc tagaaacgaa ccagcgcgga
    33481 gccccaggtg aatccaccgc caaaggcttc aagcagaacc aactgccccg gcttaatgcg
    33541 cccgtcgcgt acagcttcat ccagcgcgca cgggacagag gccgcagagg tattaccgtg
    33601 gcgatccagc gtcaccacga cattgtccat agacataccg agttttttcg ccgttgcact
    33661 gataatacgc aggttagcct gatgcggaac cagccagtcc agttgagaac ggtcaagatt
    33721 attcgccgcc agcgtctcat caacgatgtg cgccagttcc gttaccgcaa ccttgaagac
    33781 ttcgttgccc gccatcgtca gatgaattga attctctgga ttcacgcggt cggcgtttgg
    33841 cagcatcagc aattcaccat aactaccgtc ggcatgcaga tgggtggaga tgattcccgg
    33901 ctcttcagag gcagccagca ccgcagcgcc cgcgccatcg ccaaaaataa taatagtccc
    33961 acgatctgtt ggatcgcagg tgcgcgccag tacatcggaa ccgacgacca gagcatactt
    34021 caccgcccca gatttcacgt attgatcggc tacgcttaat gcataggtga aacctgcgca
    34081 ggctgctgca acgtcaaatg ccgggcaacc tttaatgccc aacatgcttt gaatctgaca
    34141 agctgcgctc gggaaagcgt gcgtagcaga cgtcgttgcc acaacgatca ggccaatctg
    34201 gtctttctca atgcccgcca tctcaattgc gcgtgtcgcc gcttcaaagc ccatggttga
    34261 aacggtttcg tttggcgcgg caatgtggcg ttcgcggata ccggtacgag tgacaatcca
    34321 ctcgtcagag gtgtccacca ttttttccaa atcggcgttt gtccgcactt gttcgggcag
    34381 atagctgcca gtaccaataa tcttcgtata catgtacgct cagtcacttt tcggttatat
    34441 accgtcactt gcaaactgcg agttcgctgg cagcgtcctg ctaccgcaga gttccgcttt
    34501 tgccaccgtc cagcagctca aaaccagctg ggtatacaga ttccaggcga gcggcaattc
    34561 gctgaggaac ttgtcgctgc accgcctgca ctgcctgttc aatcgcgacc gcaaaagctc
    34621 gctgattggc tgcaccatga ctttttatca ccgtgccgcg caatcctaac agacaggcgc
    34681 cgttatactg gtcggggttg aggtgactga atcgcctcgt caggctcttt tgtagccaac
    34741 gctttaataa cagtagccac cacgaccgtt ttttcccttc accctgagat ttcagcagag
    34801 aaaggaacat cctgacaaca ccttccatcg tctttaacgt gacatttcct gtaaagccgt
    34861 cacaaaccag cacatctgtc ttgccagtta acaactcatt ggcttcaaga tagccgatat
    34921 aattgataga agggattgtt ttaagcaccg ctgaggcctc ccgaatactg tcgagaccct
    34981 ttacttcttc ttcaccaata ttgagcaacg ccacgcgagg attgggaatt tccaccacct
    35041 cttcagccag aactgagccc ataatggcaa attgcaccaa cattgtgctg tcacaatcga
    35101 cgttggcccc taaatcgagg accaccgttt tgcccttttg ctgatgtggt aataccgtca
    35161 ccagcgccgg acgctcaatc ccctccaggg gcttgagtaa taattttgcc agccccatca
    35221 gtgccccggt attaccggca ctgacacagg cttgcgctcg accttctttc accagctcca
    35281 gggccatacg cattgaactc ccacgactag cgcggatagc ttgcgaaggc cgggcatcac
    35341 tggcgataac tgactgcgca ggaataatct gcagacgcga acgttgttca aagtcagctt
    35401 tagcaagtaa tggcgtgatg gcgtcgggat tgccgactaa aagaagagtg agttgcgaat
    35461 tagaattcag tgcctgcaat gctgcaggca ctgtcacgga agggccaaaa tcccctccca
    35521 tgacatctaa cgccaaggtt agacgtgtca aggtatcgtc gccgcccggt ttggtctttc
    35581 cccagttgcc tggggaaaat cctcactaag cttcatcacg cagacgcgtg attacttagc
    35641 gatgaccttg cggccgcggt agtaaccgtc ggcagtgata tggtgacgca ggtgtttttc
    35701 accagaagtt ttgtctacag acaggctggt gactgcggtc agcgcgtcat gggaacgacg
    35761 catgccacgt ttggaacggg ttggtttatt ctgttgtacg gccatggacc ttactcctca
    35821 attacggttt atccccgatc caacggggag caccaattac ttacgcttta agctggctaa
    35881 tacggcaaat gggtttggct tttgcacttc ttcaggcagt tcaccaaaga ccatgtccgc
    35941 ttcggacact tcacagtgtt cagaatcatg caccggaact accggcaagg cgaggatgat
    36001 ttcatcttca accattgcaa gcagatcgat ttcaccgaat tcgttaacct caatcggttc
    36061 atacgcttcc ggcagtgctt cagcctgttc gtctgaacgc acaggactaa aacaatacgt
    36121 tgtgtagacc tgatgagtaa acggcttccc gcaacgctga cactcgagcg ttaccgtcac
    36181 cttcgcatcg ccgtttaaca ctgcgagacg ttggttatcg atagcgaacg acatggagca
    36241 ttccacatca ctgtccacac tgactacgga ttcggcgacg cgctcaacct gatcaggggt
    36301 atagatcccc tggtaatcaa ggcgtttttg agccgtacga accggatcga gagtcagggg
    36361 taattttacc ttttgcatag ggcgcgcata ttaactttgt aacgtcatag agtcaaagaa
    36421 aaaggcagcc tgtggttgcc ttttgccaat aattcgcaca cattgcgggt tactgcttta
    36481 ttttcgttca gtgctgataa cgaacatcaa cctgcaaaac gaagcgcata gtttaaaatg
    36541 gctatcatca atacgctata tctgatggaa aaaaatatgc ctaaacttat tttagcctcc
    36601 acctcgccat ggcgtcgcgc tcttctggaa aaactgcaaa tctcttttga atgtgcagca
    36661 ccagaggtcg acgaaacccc acgcagcgac gaatcaccgc gacagttggt gcttcgactg
    36721 gcacaagaaa aagcgcaatc tctggcgtca cgttatccgg atcatttaat tattggttca
    36781 gaccaggtat gtgttcttga tggcgaaatc accggtaaac cgttaacgga agaaaatgcc
    36841 cgtctgcaat tacgcaaagc cagcggcaat atcgtcacct tctatactgg actggcgttg
    36901 tttaattcgg cgaatgggca tctacaaaca gaagtggagc cttttgacgt ccatttccgt
    36961 cacctgagcg aggcggagat tgataattac gtgcgtaagg agcatcccct gcactgcgcg
    37021 ggtagcttta agagtgaagg atttggcatt acgctgtttg agcgtttaga gggacgtgac
    37081 cctaacacgc tggttggttt accgctaatc gcgctgtgtc agatgttacg tcgggaaggg
    37141 aaaaacccgc tgatgggata gttgtttgcg ccggatgcgg ttacgtcgca tccggcaatc
    37201 ggtgcaggcg gcctgatgcg gcgtaaacgc cttatcaggc caaccgccca ttatcaggct
    37261 tatatgcttg tattagcgcg cgttacgcag cttttgcaaa caacgcttca aaccttcatc
    37321 catcggcgct tcgatacgca tcacctcacc ggtccccgga tgggtaaact tcaacgctgc
    37381 ggcgtgcagg aacagacgat ttaatcccgt gcctgcttca gtgagctgtc tgtcaaattc
    37441 acggtcaccg tagcgatcgt caaaggcaat cggatgaccc gcatactgtg tatgcacacg
    37501 gatctgatga gtacgtccgg ttaccgggct acaacgcacc agggtggcaa atgcatagcg
    37561 ttcttccact ttaaagcgtg tttccgacgg tttgccttcc tgactgacac gcacgatacg
    37621 ttcgccgctt tgcagaatat ttttcaataa cggcgcttga acgctcttca catgtgactg
    37681 ccactgactg cgcaccagcg ccaggtaatc tttttgcatt cctttttcac gtaattgctc
    37741 atgcagagaa cgcaacgccg agcgtttttt cgctaccagc aacacacctg aagtgtcccg
    37801 gtcgagacga tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc gcaaaccttc
    37861 gataacgccg aagcttaaac cactgccgcc atgtaccgcc gtaccggaag gtttattcag
    37921 caccaggata tgatcatctt catataagat gacgtccgcc agcgccgcca ccttttgcag
    37981 atgtggcgaa accgcctctt cttcccgctc agcaacgcga accggtggga tacgcacctc
    38041 atcacccgct tcgagtttat attcaggctt aatacgtttt ttgttcaccc gcacttcgcc
    38101 tttacgcaaa atacggtaaa tcatactttt tggtacgcct ttcaattggg tacgcaaaaa
    38161 gttgtcgata cgttgccccg cttcgtcagc ggtgatagca acaattttta cggatggagt
    38221 ctctgttttc atggtgagcg attctaatta gccaacagga ttcgcgccac tcatttttct
    38281 atgcttatat ttagttttac accttattac ttcactacgt gatcacttta ttgatggtta
    38341 ttaaaccaat caccagcaag a
//
LOCUS       DAJTNA010000016        37634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTNA010000016 DAJTNA010000000
VERSION     DAJTNA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37634
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.016"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..1987
                     /locus_tag="OO161_000706"
                     /pseudo
     CDS             1..1987
                     /locus_tag="OO161_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            2029..2706
                     /gene="ybiX"
                     /locus_tag="OO161_000707"
     CDS             2029..2706
                     /gene="ybiX"
                     /locus_tag="OO161_000707"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5461875.1"
                     /translation="MMYHIPGVLSPQEVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            2780..3046
                     /gene="ybiI"
                     /locus_tag="OO161_000708"
     CDS             2780..3046
                     /gene="ybiI"
                     /locus_tag="OO161_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5461876.1"
                     /translation="MASGWANDEAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3311..3571
                     /locus_tag="OO161_000709"
     CDS             3311..3571
                     /locus_tag="OO161_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5461877.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3799..4884)
                     /gene="hcxB"
                     /locus_tag="OO161_000710"
     CDS             complement(3799..4884)
                     /gene="hcxB"
                     /locus_tag="OO161_000710"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5461878.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5025..5987)
                     /gene="ybiB"
                     /locus_tag="OO161_000711"
     CDS             complement(5025..5987)
                     /gene="ybiB"
                     /locus_tag="OO161_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5461879.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(6015..8165)
                     /gene="dinG"
                     /locus_tag="OO161_000712"
     CDS             complement(6015..8165)
                     /gene="dinG"
                     /locus_tag="OO161_000712"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5461880.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCAGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            8285..8767
                     /gene="ybiA"
                     /locus_tag="OO161_000713"
     CDS             8285..8767
                     /gene="ybiA"
                     /locus_tag="OO161_000713"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5461881.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTR
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8999..10363)
                     /gene="rhlE"
                     /locus_tag="OO161_000714"
     CDS             complement(8999..10363)
                     /gene="rhlE"
                     /locus_tag="OO161_000714"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR5461882.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10592..11263
                     /gene="cecR"
                     /locus_tag="OO161_000715"
     CDS             10592..11263
                     /gene="cecR"
                     /locus_tag="OO161_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5461883.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     LQRSL"
     gene            11266..12261
                     /gene="hlyD"
                     /locus_tag="OO161_000716"
     CDS             11266..12261
                     /gene="hlyD"
                     /locus_tag="OO161_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5461884.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12254..13990
                     /locus_tag="OO161_000717"
     CDS             12254..13990
                     /locus_tag="OO161_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5461885.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13983..15116
                     /locus_tag="OO161_000718"
     CDS             13983..15116
                     /locus_tag="OO161_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5461886.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIHAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15127..16233
                     /locus_tag="OO161_000719"
     CDS             15127..16233
                     /locus_tag="OO161_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5461887.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16195..16605)
                     /locus_tag="OO161_000720"
     CDS             complement(16195..16605)
                     /locus_tag="OO161_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5461888.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16738..17499
                     /locus_tag="OO161_000721"
     CDS             16738..17499
                     /locus_tag="OO161_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5461889.1"
                     /translation="MPDQTQQFSFKVLTINIQKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            17496..18737
                     /gene="clsB"
                     /locus_tag="OO161_000722"
     CDS             17496..18737
                     /gene="clsB"
                     /locus_tag="OO161_000722"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5461890.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18737..19693
                     /locus_tag="OO161_000723"
     CDS             18737..19693
                     /locus_tag="OO161_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5461891.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVA
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALTQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(19729..20442)
                     /locus_tag="OO161_000724"
     CDS             complement(19729..20442)
                     /locus_tag="OO161_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5461892.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVGIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLVIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(20648..21352)
                     /locus_tag="OO161_000725"
     CDS             complement(20648..21352)
                     /locus_tag="OO161_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5461893.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(21489..21941)
                     /gene="moaE"
                     /locus_tag="OO161_000726"
     CDS             complement(21489..21941)
                     /gene="moaE"
                     /locus_tag="OO161_000726"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5461894.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALSLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(21943..22188)
                     /gene="moaD"
                     /locus_tag="OO161_000727"
     CDS             complement(21943..22188)
                     /gene="moaD"
                     /locus_tag="OO161_000727"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5461895.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(22181..22666)
                     /gene="moaC"
                     /locus_tag="OO161_000728"
     CDS             complement(22181..22666)
                     /gene="moaC"
                     /locus_tag="OO161_000728"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5461896.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22669..23181)
                     /gene="moaB"
                     /locus_tag="OO161_000729"
     CDS             complement(22669..23181)
                     /gene="moaB"
                     /locus_tag="OO161_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5461897.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIALQLDARTC
                     PCNFHPHLKK"
     gene            complement(23203..24192)
                     /gene="moaA"
                     /locus_tag="OO161_000730"
     CDS             complement(23203..24192)
                     /gene="moaA"
                     /locus_tag="OO161_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5461898.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(24180..24324)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24589..25497
                     /gene="yvcK"
                     /locus_tag="OO161_000731"
     CDS             24589..25497
                     /gene="yvcK"
                     /locus_tag="OO161_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5461899.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(25689..27710)
                     /gene="uvrB"
                     /locus_tag="OO161_000732"
     CDS             complement(25689..27710)
                     /gene="uvrB"
                     /locus_tag="OO161_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5461900.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(28289..28966)
                     /gene="bioD"
                     /locus_tag="OO161_000733"
     CDS             complement(28289..28966)
                     /gene="bioD"
                     /locus_tag="OO161_000733"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5461901.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQYAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(28959..29714)
                     /gene="bioC"
                     /locus_tag="OO161_000734"
     CDS             complement(28959..29714)
                     /gene="bioC"
                     /locus_tag="OO161_000734"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5461902.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(29701..30855)
                     /gene="bioF"
                     /locus_tag="OO161_000735"
     CDS             complement(29701..30855)
                     /gene="bioF"
                     /locus_tag="OO161_000735"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5461903.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(30852..31892)
                     /gene="bioB"
                     /locus_tag="OO161_000736"
     CDS             complement(30852..31892)
                     /gene="bioB"
                     /locus_tag="OO161_000736"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5461904.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVHGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            31979..33268
                     /gene="bioA"
                     /locus_tag="OO161_000737"
     CDS             31979..33268
                     /gene="bioA"
                     /locus_tag="OO161_000737"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5461905.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGKVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            33327..33803
                     /locus_tag="OO161_000738"
     CDS             33327..33803
                     /locus_tag="OO161_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5461906.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            33955..35238
                     /locus_tag="OO161_000739"
     CDS             33955..35238
                     /locus_tag="OO161_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5461907.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35379..37616)
                     /locus_tag="OO161_000740"
                     /pseudo
     CDS             complement(35379..37616)
                     /locus_tag="OO161_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
BASE COUNT         8819 a        10186 c         9740 g         8889 t
ORIGIN      
        1 ccggcgtggg tgcgtttttt gcgggtgaga acggtaactc caccactggc gacgccattt
       61 atatgcgtgg tgccgatacc tctaacagta tttatattga tggcattcgc gatatcggca
      121 gcgcctcgcg cgacaccttc aataccgagc aggtcgaagt gattaaaggg ccgtccggca
      181 ccgactacgg gcacagcgca ccgacgggct cgatcaatat gatcagcaaa cagccgcgca
      241 atgattccgg cattgacgcc tccgccagta ttggcagcgc ctggttccgc cgcggcacgc
      301 tggacgtcaa tcaggtcatt ggtgatacca ccgcggtgcg cctgaatgta atgggcgaaa
      361 aaacgcacga tgccggacgc gacaaagtca aaaatgagcg ttacggcgtc gccccttctg
      421 tcgcttttgg ccttggtaca gcgaatcgtt tgtatcttaa ttatctgcat gtcacccagc
      481 acaacacgcc agacggcggc attccgacca tcggtttgcc gggctattct gccccatctg
      541 cgggaacggc ggccctgaat cattccggaa aagttgatac tcataacttt tacggcacgg
      601 attccgatta cgacgattcg accaccgaca ccgccaccat gcgttttgag cacgacatca
      661 acgataacac caccattcgc aatactaccc gttggtcgcg cgtaaagcag gattacctga
      721 tgacggcgat tatgggcggg gcgtcgaata ttactcagcc caccagcgat gtgaatagct
      781 ggacctggtc acgcacgcga ataccaaaga tgtgagtaat aaaattctca ccaaccagac
      841 caacctgacc tcgacgttct ataccggttc tatcggtcat gatgtcagta ccggcgtgga
      901 atttacccgt gaaacgcaga ctaactacgg cgttaatccg gtgacgttac ccgcggtaaa
      961 tatttatcat cctgacagca gcattcatcc cggcggcctg acgcgcaacg gcgcaaacgc
     1021 caatggtcag acggatacct tcgcaattta cgcctttgat acgctgcaaa tcacccgtga
     1081 ttttgagctg aacggcggga tccgtctgga taattatcat actgaatatg acagtgccac
     1141 cgcctgcggc ggcagcggac gcggtgccat cacctgccca gctggtgtgg caaaaggttc
     1201 tccggtcacc accgtcgaca ccgccaagtc gggcaatctg gtgaactgga aagccggggc
     1261 gctgtatcac ctgacggaaa acggtaatgt ctatattaac tatgccgttt cccagcagcc
     1321 tccgggcggc aacaacttcg cccttgcgca gtctggcagc ggtaacagtg ccaaccgcac
     1381 cgattttaaa ccgcaaaaag ccaacaccag cgagattggc accaaatggc aggttctgga
     1441 taaacgcctg ttgctcaccg ccgcactgtt ccgtactgat atcgaaaatg aagttgagca
     1501 aaatgatgac ggaacttact cgcaatacgg taagaaacgt gtcgaaggct atgagatatc
     1561 cgtggccggg aatatcactc ccgcgtggca ggtgattggc ggctataccc agcaaaaagc
     1621 aaccatcaaa aacggcaaag atgttgccca ggatggttcc tcatcgctgc cgtatacccc
     1681 ggagcacgcc ttcaccttat ggagccagta tcaggcaacc gatgatatct ctgttggcgc
     1741 gggcgcacgc tatatcggca gtatgcataa aggttcagac ggcgcggtgg gaacgccagc
     1801 gtttaccgaa ggttactggg tcgccgatgc caaactgggg tatcgggtta atcgcaatct
     1861 cgactttcag ctaaacgttt acaacctgtt tgataccgat tacgtcgcct caatcaacaa
     1921 gagcggctac cgttatcacc cgggcgagcc aagaaccttc ttgctcacag ccaatatgca
     1981 tttctgattc agatgtgggg cgcaggcccc actttttgga gaaattgtat gatgtaccac
     2041 attcccggcg tgttatcgcc acaggaggtc gctcgttttc gcgaacaact ggaacaagcc
     2101 gaatgggtgg atggacgcgt caccaccggc gcacaaggtg cgcaagttaa gaacaatcaa
     2161 caggtagaca cccgcagcac gttatacgcc gccctgcaaa atgaggtgct gaacgcggtt
     2221 aaccaacatg ctttattctt tgccgcggcc ttgccgcgta ccctttccac gccgctgttt
     2281 aatcgctatc agaacaatga aacctatggt tttcatgtgg atggcgcagt acgcagccat
     2341 ccacaaaacg gctggatgcg cactgacctt tctgccacgc tgtttttaag cgatccacaa
     2401 agctacgacg gcggcgaact ggtcgttaat gacaccttcg gacaacatcg ggtaaaactc
     2461 ccggcaggtg atctcgtgtt gtatccctcc agcagcctgc attgcgtgac acccgtaacc
     2521 cgcggcgtac gagtggcatc atttatgtgg atccagtcga tgatccgcga tgataaaaag
     2581 cgcgccatgc tgtttgaact agacaaaaat attcagtcgc tgaaaagccg ctatggtgaa
     2641 aatgaagaga tcctgtcgct gctcaatctt tatcataatc tgctgcggga atggtcggag
     2701 atctgacgct tgaactgccc aggttcgtcc ctatattagc tacacttaac tgtacaagca
     2761 ttgatatggg gaggacgata tggcatccgg ttgggctaac gatgaagccg tcaacgaaca
     2821 gatcaacagt acaattgaag atgcgattgc ccgcgctcgg ggtgaaattc cgcgtggcga
     2881 aagcctgtat gaatgtgaag agtgcggtgc ccccatcccg caagcccgtc gggaagccat
     2941 tcctggcgtg cgcttatgta ttcattgtca gcaggagaaa gatttacaaa aaccagcgta
     3001 tacaggatat aatcgcagag gttcgaaaga cagccagtta cgttaactac aactggcgag
     3061 atgcatagcg agtataattt gtattttgcg tggcattccg tgcctttaac gctgctgtga
     3121 ctgccacgcg gcataaaaca agtccataag tacaacaaat aaatggttta tcagtatgtt
     3181 agatatcaat caatttattt gaacaaggcg gtcaattctc ttcgatttta tctctcgaaa
     3241 aaaaacgtga tactcatcac atcgacgaaa caacgtcact tatacaaaaa tcacctgcga
     3301 gagattaatt atgaaaacta tcaatactgt tgttgctgct atggctcttt caactctatc
     3361 atttggcgtg ttcgcggcgg aaccggtaac ggcatcccag gcacagaaca tgaataaaat
     3421 cggcgtggtt tctgccgatg gcgcatccac cctcgatgcc ctggaagcga aactggctga
     3481 gaaagccgcg gcagccggtg ccagtggata cagcatcact tctgccacca acaacaataa
     3541 attaagcggt actgcggtaa tttataagta attcgtagga cggataaggc gtttacgccg
     3601 catccggcaa cagatgcctg atgcgacgct accgcgtctt atcaggccta caaaatccga
     3661 accgttggac ggatcaggcg gttacgctac gtccagcaca agtatttccc tgtctgtttg
     3721 ccgacagacg catatgctct aaccctcatt gatcctatat tacccttgtt tgcccgtccg
     3781 ttactggacg ggcttttttt agctggctaa ctgctgacag aaagcctgca acgtctcttc
     3841 cgacatacca atctgccgcg ccgcatcaca aatggcctgc cagcttcccg catccaatgg
     3901 aatcccctgc tcctgccgtt cgcgacgcgt gttcacttcc cactcgcccg gtagcaaaat
     3961 cggcttatcg tcatcatgcg gcgaggcttt cacccactcg gcaaaggctt cagtctgcgc
     4021 gctacaatcc ggcgcgccga acagttccgg gttgatgatg atagtggtca tgcagttaag
     4081 aatggcatcg ggactggttt gtaacgtttc ctgatgcgtc gttttaccgc cagaaagcgc
     4141 cccgccaaga atttcacaca tcgctgcaag ggcgtagcct ttatgttcgg caaaggtcaa
     4201 cagcgacccc aacggcgact cctgcattac cgccggattg gtcgtcggca cgccgttaac
     4261 gtcaatcagg caacctggcg gcacggggac tcctttatgc caggcgacgc gagttttacc
     4321 aaatgcgatg gcgctggtgg cgtaatcaag caacagcggg aaattatctt tacgagggaa
     4381 aaccacacag aacggattgg tgccaaagcg gctgtcgcga ccgtggaacg gtgcgaccat
     4441 tggaataccg accacgctaa caaagtggat agagacaaac cccgccgctg cacactgctc
     4501 cgcccagtaa ccgatacggc cgatatgatg cgagttatgt agcgccacgg cagcaatacc
     4561 gtgctgatgc gctttctcaa tccccagcgc catcgcttcg tgtgccgcga cctgaccgaa
     4621 tgcgcgatcg ccatcgagcg tgactgccgc ccccgcctct ttaacgattt tggcatgatg
     4681 gttaatttgc agatgcccct gactccagga acgcacatag cttgggatca tgccaatacc
     4741 atgagaatca tgccctgcca ggtttgccgc gattaaatga tcggccacta atttcgcttc
     4801 ttgttcctcg ctacccatct gacgaaatac agcctgaata aaactgtgca gcgtctgagc
     4861 atcaaagcga tgaccacttt ccatggttaa ctcctgttta tgttatgtgt ttgttgtttt
     4921 tttatgctgc acgccgggcg cgggaaggtc aataaagatg caggaatttc atattgcggt
     4981 ggggtaaaag ccggatgaca tggctcatcc ggctgagaaa agaattaaaa tgcctgatta
     5041 acgcgcgcca ggccgtcact gatagttgcc gcttcacccg tagcctccag gcagcaagcc
     5101 atctggattt tcagcgattc gggaatcggt tcgctgccag caaggcaacg ctcaatccac
     5161 tgcgccgtgg tttctgggtc ttttgcttgt ggcagtaact cgctaccagc agtgtcctgt
     5221 ttttcataca gcacccgcat cccttcacgg tcaatgagat tgatctgcgg gcagcgctgc
     5281 ggattagcat acacttcgcc ttcagtacca tgcattagta gcgcccgccc gccgatatcg
     5341 ctaaagaact tcgcgactcg cccgatgtat tccggatgcg aaacgctgga aagacgcagc
     5401 gcctcacctt cggcaaatgg cgtcgccagt ttcgccaggg tatgtgcact gttacgcacg
     5461 cccatccgcc agcgcatcgc cagttgtttt tccagcggcg ggcaaaacgc gccgacaggc
     5521 ataaacaccg gttgatgttc gtcgagcttc gcctgcgcct gcccgccgtg caacgttggt
     5581 gtaataccca tcagttcgaa aatggtttct gtcagcacgc gagttggatc ttcgctaacc
     5641 ccgtgaacca ccacaggaaa accgagtttg tgcaggagaa tagccaacaa cggcgtcagg
     5701 ttggcctgtt tacgcgcgcc gttgtaactg gggatgacaa tcggcatcgg cttgcctgca
     5761 ggcggcgtca gcttgatggt gtgattttgc atggcttcgt aaaagccgag catctctgct
     5821 tccccttccc ctttgatacg tagcgcaatc aatacgccgc ccaactcaag gtcagggact
     5881 tcaccattga gcatatgggc atacagaccg cgcgcagtat cccggtctaa atcgcgcgcg
     5941 tggtttttcc cgcgcccgat ctctttaatg attttgcgat agtccattta cgactcctta
     6001 cctgactcac atcattaacg ccgccgacgg cgtggggatt tcgttttttc tttcttttta
     6061 actatacctt cagggacttc cggttgctct atcggaaata ccggtaatgc atccagtagt
     6121 cgcttgccat aatttttggt cagcaagcgt ttgtcgtaga taaccacttc gccccagcaa
     6181 ccgtggcttc gaatcagtcg cccaacctgc tgaatcaggt taaacgaggc gctcggcagg
     6241 ctttgcacct caaacggata gcggttgagg cttttcagcc attccccttc ggtgatcacc
     6301 accgggctgt cgatgggcgg aaaagcgatt ttgtggatat gcacctggct gagcagatca
     6361 cctttcaaat caagcccttc ggcaaatgac tgtaagccca ccagcacact acgctcaccg
     6421 ttggcaacgc gtttgcggtg cagttcaact aagcgataac gcggctgatc gccctgcacc
     6481 agcagcatca ggcgtaaatc cgtcacatag tcgagaaagc gctgcatcgc ccgtccgctg
     6541 gcaaacagta ccaacatacc gagatgtttt ttgctctcca cctgctcacg gaaaaaggcc
     6601 gccatttcgg caatatgctg ctcttcgttg tcgatggaag gctcaaagcg catccgggga
     6661 ataacaattt tgccctgttc gcagtggtta aagggggaat ccagcgccac aaaacggtcg
     6721 cccgctttct ctttcagccc gctcatctcc tgcaaacgcg aaaaactgtt cagcgaacgc
     6781 aaggttgcgg aggtgacaat aatgtgcgga atactgcgcc acagcagcct ttccagctga
     6841 tcgctgacac gtattcccgc gcagtgaaac cagagatgca gctgcccttc gcgctcttcc
     6901 cgcgtcgccc atttggtcac cggcgcaccg gaagattgcg ccagagacgc cagccgccag
     6961 agtttgcttt gcgcctcgaa catccccaac gcgcggttca tctgcaaaat caaccgatgc
     7021 agacgcacaa tatcgtggct gccggttttc tcacttaaat cgttaagaaa taactccgcc
     7081 agaccacgca gcatctcggt gagttttgcc agccgctggc agatctccag cacttcatca
     7141 ggcagttcgc ccatcgcaaa acggtgctct gcctcctgcc cggcaggcat gtagagattg
     7201 agaatgttgt ttaacgaggc gataagctca tacaactctt cacaatgcgc attcaaacgt
     7261 tcagggatcg ccagcggtgg gatggtcttc gggcgaaact gctccatgca ggtagcgacc
     7321 agtttcgtga acaagtccag ctgtagtcga taccagggcg cggtgatttc agcgctcatc
     7381 tccagcgcat cccgggccac atctggcagg tgatgacctt cgtccagcac cagcagcaga
     7441 tttttcgggt caggcagtac ggcttcgctt tccatcgccg ccatcaccag cgcatggttt
     7501 gccaccacca cttccgcttc ctgaatctcc cgacgagcaa caaaaaacgg gcattcacga
     7561 tagtagtaac agttacggtt gaggcagctg gctttgtcgg tactcaaacg acgccagaga
     7621 tcatcatcta tagcgatatc cgtatgatcg cgcagaccat cccatttata agtgtcgaga
     7681 tcgcccttca gcttcgcaca acgtttttgc tcttcctgat tatttggcgt cagttcgtcg
     7741 tcaagaaacg ccagcagatc ctgttgcgtg ggttcagtgc tggcgagcgc cgtcagatta
     7801 cgcggacaaa cgtagcgccc acgcccgaaa gcggcagtga atttaagatc gggaatgatc
     7861 tttttcagca gcggtaaatc tttgctgtaa atctgatcct gcaacgccac gttggcggta
     7921 ctcaccacca gcgttttttg ctcttcgcgg gcaatggcga tgccggggat caaataggag
     7981 agcgttttcc caacgccggt gggggcttca atcgccagat gccgcccttc ttctccggcc
     8041 agcgttttgg cgacgtccgc aatcatctgc cgctgcggcg cacggggaat aaagtcgggg
     8101 atctgttcct gaagcgcctt ataccaggcg gcaatttgcg ctttaagcgc ggcggttaat
     8161 gccatgagaa aacctgaaat actgtataaa cagccaatat tgtggcattt ttcggttttc
     8221 aggggtaatg gtttttctgt ttctggaaag cggctcgcaa taaccaccaa cataatgcgg
     8281 tttaatgccc gttcgagcac aaaggatcca acacgtcatg caagacacca tcataaattt
     8341 ctacagcacc agcgatgact acggtgattt ttctaacttt gccgcctggc ccatcaaggt
     8401 tgacgggaaa accaggccca cctccgaaca ctattttcag gcacaaaaat tccttgatga
     8461 aaaataccgc gaagagatcc gtcgggtttc ttcccccatg gtcgccgctc gcatggggcg
     8521 taatcgttcc aagccacttc gtaaaaactg ggagtcggtt aaagaacagg tgatgcgtaa
     8581 agcacttcgt gctaaattcg aacagcatgc agaactgcgt gcgctcttgc tggcaaccgc
     8641 gcccgcaaaa ctggttgagc atacggaaaa cgatgcttac tggggagacg gtggtaatgg
     8701 taagggcaag aatcgactgg gctacctttt aatggagttg cgcgaacaat tggctataga
     8761 gaagtaacgt ctcggtggct tatgggcggt aaaacagata aattttttcc cgtgttctca
     8821 aattgctgac aagtgcttgt tgtgaaatgc cggatgcggc gtaaacgcct tatccggcct
     8881 acaaaagcat gcaaattcaa tatattgcag agttaacgta ggcctgataa gcgtagcgca
     8941 tcaggcagtt ttgcgtttgt tcatcagcct gatgccgggc atagcccggc ataaaagatt
     9001 actgcgcagc ggcaggttta cgcggacggc ggcgacgttg ttgttcgcct gccggtttgg
     9061 catcgccgag gcggcgagac ggtttttctg caggtttcgc gcttgcagat tttgcgccac
     9121 cttccccacg gcgtggttgc tgttgaccgc gaccaccacc ttgcccacga ccgccgccgc
     9181 cacgttgctg gcgaccgttc tggatcggtt cggctttgat tgacgggtcc ggctcatagc
     9241 ccggaatcgc aatgcgcggg atctcttttt tcagcagttt ttcgatatca cgcagcagtt
     9301 tgtgttcatc aacacacacc agcgacaacg cttcaccggt agcagccgca cgaccggtac
     9361 gcccgatacg gtggacataa tcttccggta cgtttggcag ttcatagttg acgacgtgcg
     9421 gcaactcttc gatatccaga ccgcgcgcag cgatatcggt cgccaccagt acgcgaatgt
     9481 cactcgattt aaaatcagcc agcgcacggg tgcgcgcacc ttgcgattta ttcccgtgga
     9541 tcgccgcact acggatgcca tctttattga gctgctcagc cagatggtta gcgccgtgtt
     9601 tggtacgggt aaacaccagc acctgctgcc agttcccttt accaatcatg tgcgacagca
     9661 attcgcgttt gcgtttctta tcgacaaagt gaacgtgctg ggtcacctga tcagacgcgg
     9721 tattgcggcg tgccacttcg atttccagcg ggttgtgcaa cagtttttcc gccagggctt
     9781 taatatcgtc agagaaggtc gcagagaaca gcaggttctg gcgtttcgct ggcagttttg
     9841 ttaacacgcg acggatatcg tggataaagc ccatgtcgag catgcggtca gcttcatcga
     9901 ggacgaggat ttcaacctga tccagcttca ctgcattttg atgttccagg tccagcaaac
     9961 gtcccggtgt tgccaccaga acatcaacgc cgccgcgcag cttcatcatt tgcgggttaa
    10021 tgctgacacc gccaaacacc accagcgaac gaatgttcaa atatttgctg taatcacgga
    10081 cgttttcgcc aatctgcgca gccagttcac gggtcggggt aagaatgagc gcacgtaccg
    10141 gacgacgccc tttggcgtgc ggctggcgag tgatcaggtg ttgcaacagc ggcagcgtaa
    10201 agcccgctgt tttaccggta ccggtctggg cgctagccat caggtcgcgg ccttccagca
    10261 ccgcagggat cgcctgctgc tgaatagggg tgggttcacg gtaaccctgc tcggcaacgg
    10321 cgcgcaggat atcagggctt aaacccaaag aatcgaaaga cataactact ccgaaccgcc
    10381 ccgaccgtta ccggtgtagt tttcagggag atactgaaaa ggaaaatgac aaaaaccaca
    10441 atgtcatgag ggagcggagt gtagcagttt ttgtgacgca gcgcataaat tatccctgtg
    10501 actggcgttg ctaaaattct tagccatact ggacaactcc cctatggagt cataatctta
    10561 atcaatcatt tgattaagaa tggactgtgc gatgaataat cctgccatga caatcaaggg
    10621 tgaacaggcg aaaaaacagc tgattgctgc cgcactggcg cagtttggtg aatatggaat
    10681 gaacaccacc actcgcgaga tagccgccca ggccgggcag aatatcgctg ccatcaccta
    10741 ctacttcggt tcgaaagaag atttgtacct cgcctgcgcc cagtggattg ccgattttat
    10801 tggcgagcag ttccgtccgc atgccgagga agccgaacgc ttgttcgcgc aaccacagcc
    10861 tgatcgggct gccatccgtg aactgatcct tcgcgcctgc aggaacatga ttaagctgct
    10921 cacccaggat gacaccgtca acctcagcaa gtttatctcc cgtgagcagc tctctcccac
    10981 ggcagcctac cacctggtgc acgaacaggt gattagtccg ctacacagcc acctgacacg
    11041 cctgattgcc gcctggaccg gctgcgacgc caatgacacc cgcatgatcc ttcataccca
    11101 tgcgttgatt ggtgagattc tggcgttccg tcttggcaaa gaaacaattc tgttacgtac
    11161 cggctggacc gcgttcgatg aagaaaagac cgaactgatc aaccagacgg tgacctgtca
    11221 tatcgacctg attttgcaag gattattgca aaggagtttg tagtgatgaa aaaacctgtc
    11281 gtgatcggac tggcggtagt ggtacttgcc gccgtggttg ccggaggcta ctggtggtat
    11341 caaagccgcc aggataacgg cctgaagctg tatggcaacg tggatattcg tacggtgaat
    11401 cttagtttcc gtgttggggg gcgcgttgaa tcgctggcgg tggacgaagg tgatgcgatc
    11461 aaagcgggcc aggtgctggg cgaactggat cacaagccgt atgagattgc cctgatgcag
    11521 gtgaaagcgg gcgtctcggt ggcacaggcg cagtatgacc tgatgcttgc cgggtatcgc
    11581 gatgaagaaa tcgctcaggc cgccgcagcg gtgaaacagg cgcaagccgc ctatgactat
    11641 gcgcagaact tctataaccg ccagcaagga ctgtggaaaa gccgcaccat ttcggcaaat
    11701 gacctggaaa atgcccgctc ctcgcgcgac caggcacagg caacgctgaa atcagcacag
    11761 gataaattgc gtcagtaccg ttccggtaac cgtgaacagg acatcgctca ggcgaaagcc
    11821 agcctcgaac aggcgcaggc gcaactggcg caggcggagc tgaatttaca ggactcaacg
    11881 ttgatagcac cgtctgatgg cacactgtta acgcgcgcgg tggagccagg cacggttctc
    11941 aatgaaggtg gcacggtgtt taccgtttca ctaacgcgtc cggtgtgggt gcgcgcttat
    12001 gttgatgaac ggaatcttga ccaggcccag ccggggcgca aagttctgct ttataccgat
    12061 ggtcgcccgg acaagccgta tcacgggcag attggtttcg tttcgccgac tgctgaattt
    12121 accccgaaaa ccgtcgaaac accggatctg cgtaccgacc tcgtctatcg cctgcgtatt
    12181 gtggtgaccg acgccgatga tgcgttacgc cagggaatgc cggtgacggt gcaattcggt
    12241 aacgaggcag gacatgaatg atgccgttat cacgctgaac ggcctggaaa aacgctttcc
    12301 gggcatggac aagcccgccg tcgcgccgct cgattgtacc attcacgccg gttatgtgac
    12361 ggggctggtg gggccggatg gtgcaggtaa aaccacgcta atgcggatgc tggcgggatt
    12421 actgaaaccc gacagcggca atgccacgat gattggcttt gatccaatca aaaacgacgg
    12481 cgcgctgcac gccgtgctcg gctatatgcc gcagaaattt ggtctgtatg aagatctcac
    12541 ggtgatggag aacctcaatc tgtacgcgga tttgcgcagc gtcaccggcg aggcacgtaa
    12601 gcaaactttt gctcgcctgc tggagtttac gtctcttggg ccgtttaccg gacgcctggc
    12661 gggcaagctc tccggtggga tgaaacaaaa actcggtctg gcctgtaccc tggtgggcga
    12721 accgaaagtg ttgctgctcg atgaacccgg cgtcggcgtt gaccctatct cacggcgcga
    12781 actgtggcag atggtgcatg agctggcagg cgaagggatg ttaatcctct ggagtacctc
    12841 gtatctcgac gaagccgagc agtgccgtga cgtgttactg atgaatgaag gcgagttgct
    12901 gtatcagggg gaaccaaaag ccctgacaca aaccatggcc ggacgcagct ttctgatgac
    12961 cagtccacac gagggcaacc gcaagctgtt gcaacgcgcc ttgaaactgc cacaggtcag
    13021 cgacggcatg attcagggga aatcggtacg tctgatcctc aaaaaagagg ccacaccaga
    13081 cgatattcgc catgccgacg ggatgccgga aatcaacatc aacgaaacta cgccgcgttt
    13141 tgaagatgca tttattgatt tgctgggtgg tgccggaacc tcagaatcgc cgctgggtgc
    13201 aatattgcat acggtagaag gcacacccgg cgagacggtg atcgaagcga aagaactgac
    13261 caagaaattt ggtgattttg ccgccaccga tcacgtcaac tttgccgtta aacgtgggga
    13321 gatttttggt ttgctggggc caaacggcgc gggtaaatcg accaccttta agatgatgtg
    13381 cggtttgctg gtgccgactt ccggccaggc gctggtgctg gggatggatc tgaaagagag
    13441 ttccggtaaa gcgcgccagc atctcggcta tatggcgcaa aaattttcgc tctacggcaa
    13501 cctgacggtc gaacagaatt tacgcttttt ctctggtgtg tatggcttac gcggtcgggc
    13561 gcagaacgaa aaaatctccc gtatgagcga ggcatttggc ctgaaaagta tcgcctccca
    13621 cgcgaccgat gaactgccat taggttttaa acagcggctg gcgctggcct gttcgctgat
    13681 gcatgaaccg gacattctgt ttctcgacga accaacgtcc ggcgttgatc ccctcacccg
    13741 ccgcgaattt tggctgcata tcaacagcat ggtagagaaa ggcgtcacgg tgatggtcac
    13801 cactcacttt atggatgaag cagaatattg cgaccgcatc ggcctggtgt accgtgggaa
    13861 attaattgcc agcggcacgc cggatgattt gaaagcgcag tcggccaacg acgagcaacc
    13921 cgatcccacc atggagcaag cctttattca gttgatccac gactgggata aggagcatag
    13981 caatgagtaa cccgatcctg tcctggcgtc gcgtacgggc gctgtgcgtt aaagagacgc
    14041 ggcagatcgt tcgcgatccg agtagctggc tgattgcggt agtgatcccg ctgctactgc
    14101 tgtttatttt tggttacggc attaacctcg actccagcaa gctgcgggtc gggattttac
    14161 tggaacagcg tagcgaagcg gcgctggatt tcacccacac catgaccggt tcgccctaca
    14221 tcgacgccac catcagcgat aaccgtcagg aactgatcgc caaaatgcag gcggggaaaa
    14281 ttcgcggtct ggtggttatt ccggtggatt ttgcggaaca gatggagcgc gccaacgcca
    14341 ccgcaccgat tcaggtgatc accgacggca gtgagccgaa taccgctaac tttgtacagg
    14401 ggtatgtcga agggatctgg cagatctggc aaatgcagcg agcggaggac aacgggcaga
    14461 cttttgaacc gcttattgat gtacaaaccc gctactggtt taacccggcg gcgattagcc
    14521 agcacttcat tatccccggt gcggtgacca ttatcatgac ggtcatcggc gcgattctca
    14581 cctcgctggt ggtggcgcga gaatgggaac gcggcaccat ggaggctctg ctctctacgg
    14641 agattacccg cacggaactg ctgctgtgta agctgatccc ttattacttt ctcgggatgc
    14701 tggcgatgtt gctgtgtatg ctggtgtcag tgtttattct cggcgtgccg tatcgcgggt
    14761 cgctgctgat tctgtttttt atctccagcc tgtttttact cagtaccctg gggatggggc
    14821 tgctgatttc cacgattacc cgcaaccagt tcaatgccgc tcaggtcgcc ctgaacgccg
    14881 cttttctgcc gtcgattatg ctttccggct ttatttttca gatcgacagt atgcccgcgg
    14941 tgatccacgc ggtgacgtac attattcccg ctcgttattt cgtcagcacc ctgcaaagcc
    15001 tgttcctcgc cgggaatatt ccagtggtgc tggtggtaaa cgtgctgttt ttgatcgctt
    15061 cggcggtgat gtttatcggc ctgacgtggc tgaaaaccaa acgtcggctg gattagggag
    15121 aagagcatgt ttcatcgctt atggacgtta atccgcaaag agttgcagtc gctgctgcgc
    15181 gaaccgcaaa cccgcgcgat tctgatttta cctgtgctta ttcaggtgat tctgttcccg
    15241 ttcgccgcca cgctggaagt gactaacgcc accatcgcca tctacgatga agataacggc
    15301 gagcattcgg tggagctgac ccaacgtttt gcccgcgcca gcgcctttac tcatgtgctg
    15361 ctgctgaaaa gcccacagga gatccgccca accatcgaca cacaaaaggc gttactcctg
    15421 gtgcgtttcc cggctgactt ctcgcgcaaa ctggatacct tccagaccgc gccgttgcag
    15481 ttgatcctcg acgggcgtaa ctccaacagt gcgcaaattg ccgccaacta cctgcaacag
    15541 atcgtcaaaa attatcagca ggagctgctg gaaggaaaac cgaaacctaa caacagcgag
    15601 ctggtggtac gcaactggta taacccgaat ctcgactaca aatggtttgt ggtgccgtca
    15661 ctgatcgcca tgatcaccac tatcggcgta atgatcgtca cttcactttc cgtcgcccgc
    15721 gaacgtgaac aaggtacgct cgatcagcta ctggtttcgc cgctcaccac ctggcagatc
    15781 ttcatcggca aagccgtacc ggcgttaatt gtcgccacct tccaggccac cattgtgctg
    15841 gcgattggta tctgggcgta tcaaatcccc ttcgccggat cgctggcgct gttctacttt
    15901 acgatggtga tttacggttt atcgctggtg ggattcggtc tgttgatttc atcactctgc
    15961 tcgacacaac agcaggcgtt tatcggcgtg tttgtcttta tgatgcccgc cattctcctt
    16021 tccggttacg tttcgccggt ggaaaacatg ccagtatggc tgcaaaacct gacgtggatt
    16081 aaccctattc gccactttac ggacattacc aagcagattt atttgaagga tgcgagtctg
    16141 gatattgtgt ggaatagttt gtggccgcta ctggtgataa cggccacgac agggtcagcg
    16201 gcgtacgcga tgtttagacg taaggtgatg taacttctta tctttcgcca gcaaagacac
    16261 taccgccggg ccggcaagga ttgccagccc tgcaattgcc agcaaaaagt tgttttccag
    16321 tacgcgggac aacagccaca gcacaatcag cgctgcgcca aaataccagg tcgtggtcag
    16381 ctcttccagc acgtctgcca cctcacgcca gcgttgctcg gggtggcgct gaaacaccaa
    16441 catcatcact accgtgacac aatacagtgc gcataatccc acgccgacgt gaaccagagt
    16501 cttactcatc cagcccacca ccagcagcgc cactaccagt aaatgcaaca taatctgcca
    16561 gcaacttaga cccgttgcga cacgaacacg ttgttgccac ttcatggctt ctctcctgaa
    16621 gaatttatct ctacttactc agagtaccga actttacgga aaattccgta agaccgcacc
    16681 tttttgtgac gccgactaca ctatttcaat gagataagag gcaaagacag gaaaattatg
    16741 cccgatcaaa cacaacaatt ttcgttcaag gtgctcacca tcaacattca aaaaggtttt
    16801 accgcgttta accgacgctt cattttgccg gaacttcgcg acgcggtgcg taccgtcagc
    16861 gccgatattg tttgcctgca ggaagtgatg ggcgcgcacg aagttcatcc gctgcatgtg
    16921 gaaaactggc ccgatacctc gcactacgag tttctcgccg acactatgtg gagcgatttt
    16981 gcctacggtc gcaatgccgt atacccggaa gggcatcacg gcaacgccgt actgtcgcgt
    17041 tatcccattg aacattatga gaatcgcgat gtttcggtcg atggtgcgga aaagcgcggc
    17101 gtgctctact gccgcattgt gccgccgatg accggaaaag cgattcatgt gatgtgcgta
    17161 catctgggcc tgcgtgaggc gcaccgtcag gcgcagcttg cgatgctcgc cgaatgggtg
    17221 aatgagctac cggacggcga accggtattg gtggcgggtg atttcaatga ctggcggcaa
    17281 aaagctaatc atccgttaaa agtgcaggcc ggactggatg agatttttac ccgcgcccac
    17341 ggacgcccgg cgcgcacgtt tccggtgcaa tttcctctac tacgactgga caggatctac
    17401 gtcaaaaatg ccagcgccag cgcgccaacc gcgttgccgc tgcggacatg gcgacacctt
    17461 tctgatcatg cccctttaag tgcggagatt catttatgaa atgtagctgg cgcgaaggca
    17521 ataagatcca gttgctggaa aacggcgagc aatattatcc cgcggtgttt aaggcgattg
    17581 gcgaggcaca agaacgcatc attcttgaaa cgtttatctg gtttgaggat aacgtcggca
    17641 aacagctaca tgcggcacta ctggcagcag cgcaacgcgg ggttaaagcg gaagtcttgc
    17701 tggatggcta cggttcgccg gatctcagcg atgagtttgt caataaactg acggcagctg
    17761 gcgtagtgtt ccgctactac gatccccgcc ctcgcctttt tggtatgcgc accaatgtgt
    17821 ttcgccggat gcatcgcaaa attgtggtga tcgacgcgcg tatcgccttt attggcgggc
    17881 tgaattactc cgccgagcat atgtccagct acggtccaga ggctaaacag gattacgcgg
    17941 tacgccttga agggccgatt gtcgaagata ttctccagtt tgagctggaa aacctgcctg
    18001 gacagagcgc ggcacggcgc tggtggcgac gtcatcacaa agcggaagag aaccgccagc
    18061 cgggagaagc gcaggtattg ctggtctggc gcgataacga agaacatcgc gatgatattg
    18121 aacgccatta tctgaaaatg ctcactcagg cgcggcggga agtgattatc gccaacgcct
    18181 acttcttccc cggctatcga tttttacacg ccttgcgtaa agcggcacgg cgcggggtgc
    18241 ggatcaaact gatcattcag ggcgaaccgg atatgccgat tgtcagagtc ggtgcgcgct
    18301 tgctgtataa ctatctggtt aaaggcggcg ttcaggtttt tgagtaccgc cgccgcccgc
    18361 tccacggcaa agtggcattg atggacgatc actgggcgac agtagggtcc agtaatctcg
    18421 atccgctcag tttgtcactg aatctcgaag caaatgtcat catccacgat cgtcatttta
    18481 accagacgct gcgcgataat ctgaacggca ttattgccgc agattgtcag caggtggatg
    18541 aaaccatgct gcccaaacgc acctggtgga acctgaccaa aagcgtgctg gcgttccact
    18601 ttttacgcca cttcccggcg ctggttggct ggcatccggc acacacacca cgtctgacgc
    18661 aggttgatcc ccccgcacaa ccgacaatgg aaacgcagga tcgggtagaa actgaaaaca
    18721 cgggggtaaa accctgataa gtaaatcaca cccgcgctgg cgcttagcaa agaagatcct
    18781 cacctggctg ttttttatcg cggtgattgt gttgctggtg gtctacgcca aaaaagtgga
    18841 ctgggaagag gtctggaagg tcatccgcga ctacaatcgc gttgcgctgc ttagtgcggt
    18901 cgggctggtg gtcgtcagct atctgattta cggctgctat gacctgctcg cccgctttta
    18961 ctgcggtcac aaactggcga agcgccaggt gatgctggtg tcgtttatct gctacgcctt
    19021 caacctgacg ctcagtacct gggtcggcgg cattggtatg cgctatcgtt tgtactcccg
    19081 actagggtta ccgggcagca ctattacgcg gattttctcg ctcagtatta ccaccaactg
    19141 gctgggctat attttactgg cagggattat ctttaccgca ggcgtggtgg agttgccgga
    19201 tcactggtat gtcgctcaaa ctacgctgcg tattctcggc attggcttac tgatgattat
    19261 cgcggtttat ttgtggtttt gcgctttcgc gaagcaccgc catatgacca tcaaagggca
    19321 aaagctggtg ctgccttcat ggaaattcgc cctcactcag atgctgattt ccagcgttaa
    19381 ctggatggta atgggggcga ttatctggct gttacttggt caaagcgtga actatttctt
    19441 tgtactgggc gtgttactgg ttagtagtat tgctggcgtc atcgtgcata ttccggcggg
    19501 gatcggtgtg ctggaagcgg tgtttatcgc actactggct ggggagcata cctcgaaagg
    19561 gagcattatc gccgcgctgc tcgcttatcg cgtcctgtat tactttatcc cgctgctgct
    19621 ggcgctgatc tgttacctgc tactggaaag ccaggcgaag aagctacggg cgaaaaatga
    19681 agcggcgatg tgaagtttag ttaagttctt tagtatctgc attcacgact aatgaaaaaa
    19741 acgcgtatgc ctttgccaga caagcgttat agctatcgcg gcaagtatcc caaagaagcc
    19801 gataaaatag taatagagtg ttaatgcacc tgtaaaagcg caacgaaccg gactccacaa
    19861 ctcttcacta tggcattttc ctgccagttc aaggagctta tttcgtccat gcgagataat
    19921 gcctggccat aacacaatca ctaagcaact tattacccaa atgggccgtt cactcattaa
    19981 tgccgcattc acgatgatta ccagggccaa ccctgtcaac gtcatcataa tgataaaacg
    20041 gtgagaacca ggatcaacat taaataataa accggccagc atactgatta aaaacatcgc
    20101 gcccgcagtg ctgaacacaa tgataatggc atatacagga actaaacttg caacgacaat
    20161 cgccaccaaa gagagtaaaa acagatatat tacgcctgcg atgacggcat ttatcttgtt
    20221 gatatcctta cttaagccat acatacaaac taataataac cctatgccga catacattgt
    20281 tggtgaatca tatttggttg ccgtaatttc gagatactta gctccccagg ccagtcctgc
    20341 ggttgccacc aaacctaaag cggtaatgag ccatataccg tttaatatcc tggtctcgtg
    20401 ctgaaaatct aatttattgc tgttttgtga gtatgattcc attcttttag ttgtccttag
    20461 aaaaaatatt aatattcacc atcaataaat ttaaacccac gcatacaaaa tacttttgta
    20521 tttatggata cggaaaacgc ttccgctcgg ccataaggcg tattttatac ctggtacttg
    20581 ttaagtgtcg tttttagtaa gaaaagaaat cccgacacat ggccgggata ttgatggtga
    20641 aaaagaatta acggcggttg ccaaaaattc gcagcaacat caggaacagg ttgatgaagt
    20701 ccagatacaa ggttaacgcg ccaagaatgg aatatttgcg caggttcgac gtgtcgcggg
    20761 tatcaatctg ctcacccata tttttcaatt tctgagtgtc atacgccgtc aatccgacaa
    20821 agacaatcac gccgatgtag gtaactgccc acatcaatgc ttcgcttttc agccagaagt
    20881 tgaccagcga tgccagcaca atgccgatta acgccataaa cagcatattg ccaaagccac
    20941 ttaaatcgcg cttcgtggtg taaccgtaca ggctcattgc accgaacatc ccggcagtaa
    21001 cgacgaaagt actggcgata gaagcagcgg tatagacaat gaatatactg gaaagcgtaa
    21061 gacccgtcag cgccgaataa agcataaaga gcatcgtcgt tacacctgcg ctcagctttt
    21121 gaatcatcgc tgataacaca ataaccaatg ctaattgcgc gatgatcaga ccgattaaaa
    21181 agacacggtt agtgaacaac agctccatca cggccgcgga attagccgca taccaggcaa
    21241 caaatgcggt cagcaacaag ccaacggtca tccagccata gacttgcgcc atataagttt
    21301 gcaagccagc ccggggttgt acgattgaat cagaacgtgg gaatctgtcc atgacgatct
    21361 cctgaagata taaggaatat cttaaggata ctgcaaaatg atgaggctgt gcatcgacgc
    21421 agcgtaaacg catgtactga gcggtgaaat tgccggacgc agcggtgcct tatccggcta
    21481 acaaaaaact accagcgttt tgccgcctgc tgatcgctct cccgagcttc aacccagcgg
    21541 tcgccttccg gcgtggcttc gcgcttccag aacggtgcgc gggttttgag ataatccata
    21601 ataaactgcc cggcttcaaa cgcactgctg cgatgcgcac tggtgacacc gacaaaaacg
    21661 atttcatcgc ccggccataa ttccccgatg cggtgaatca cagtgacgcg ccccagcggc
    21721 cagcggttac gcgcttcatc aacaatttct gccagtgctt tttcagtcat ccccggatag
    21781 tgttcgaggc ttaatgcttt gacgctgtca cccaggttat ggttgcgcac cttaccagta
    21841 aaggtgacta ccgcaccgtc ttcgtcacgc tccgccagcc acgggtactc ttctcctacg
    21901 ctgaacggct gcggaccaac aacaattttg gtttctgcca tcttaacctc cggttaccgg
    21961 cgggaagaaa gctacttcgt cgccgtcagt cagcggatgg tcaaaactca ccagcgtctg
    22021 gttgacggca gccagtaatt tgccatcttc cagcgccagc gcccagcgat cgctctgcgc
    22081 agccatgtgc tggcgtaacg cttcaacagt tgggaaatcc gcagccactt cggttgcatc
    22141 tgttcccacc aactcgcgca cctgtgcgaa aaaaagaact ttaatcatcc gcttccacct
    22201 taaagtcacc cgacttgccg ccgctcttcg ccagcaaacg taccggacca atcaccatat
    22261 ctttttgcac cgctttgcac atgtcataaa tggtcagcgc cgccacggag gccgcggtta
    22321 atgcttccat ttcgacaccg gttttcccgg tcaggcggca taaggtttct atacgcaccc
    22381 gattgtgctc cggctcggcc tgtaaattga cttcaacttt gctgagcatc agcggatgac
    22441 agagcgggat cagatcccag gtgcgttttg ccgcctgaat accggcaata cgcgcagtgg
    22501 caaatacgtc gcctttgtgg tggcgaccat caataatcat cgccagcgtc tcgctgcgca
    22561 tggtgacaaa ggcttccgcc cgcgcttcac gcacggtttc cgctttggcg gagacatcca
    22621 ccatgtgcgc ttcgccagcg gcgttgatat gggtcagttg cgacatactt atttcttcaa
    22681 atgtggatgg aaattacacg gacacgtacg ggcatccagc tgcagcgcga tgatattttc
    22741 ccatgcggta cggcacgctt tggtcgaacc cggcatggcg aaaatcagcg ttttgttggc
    22801 gacgcccgct accgcacgag attgcaacgt ggaagtgcca atctcttcaa acgacaacat
    22861 ccggaacact tcaccaaaac cttcaacttc acggtcgaac aacggcagca atgcttcggg
    22921 agcctgatca ccttccgtca ggccagtacc acccgtaatc aataccactt gtacatcgtc
    22981 gctggcgatc cacgcagata cctgagcgcg aatagcgtag cggttttctt tcacaatggc
    23041 tttatcgaca acgtgatggc ccgcttcttg cgccgaatcg cgcagatagt gaccggaggt
    23101 atcgtcttct tcaccgcgac gattagaaac cgtaagaata gcaatacggg tcgggataaa
    23161 ttcagtgctt acctgactca tctgatctct ccttttgacg ttttagccgc caatgtacga
    23221 taaattttgc gtaataccgg tgttgttttg atgcaggaaa tgggtctgtt tcttctcccg
    23281 cagcgccgct gaaatacgcg cttccagcgc ctgttgctgg gtatcgtctt ccagcagatc
    23341 gcgcaggtta acgccgcctt caccaaacag gcagagatgg agtttaccaa tggaggaaac
    23401 gcgcaggcgg ttgcaagtgg cgcagaagtc tttttcatac ggcatgataa ggccaatctc
    23461 tccggcgtaa tctggatggc aaaagacttg cgcgggaccg tcgctgcgtt gacgtaattg
    23521 gtggatccag ccgcgacgca gtagctcgtc acgcagaacc tgaccagaga tgtgatgctt
    23581 acggaagagc tcgctgccct cgcccgtttc catcagttcg atgaaacgca gctggatagg
    23641 gcgatgctgg atccagttca gaaaggtgtc gagctggtga tgattaacat cacgcatcag
    23701 cacggtattg actttgacct tctcaaaacc ggcctcaaat gcagcatcaa tccctgccat
    23761 gacctggttg aatttatcct gcccggtaat agcgtgaaac tggcgggcgt ccagactgtc
    23821 gacactgacg ttaatgccag taagtcccgc atcgcgccag ttcgccacat cgcgttccag
    23881 acggtaacca ttggtggtga ccgcaatctg gcggatagcg tcgttttccc gcacagcggc
    23941 gatgatatcg gtaaagtcgc ggcgtaaaga cggctcacct cctgtcaggc gcactttttc
    24001 ggtgcccaga ctggcgaagg cgcgcgtaac ccggcgaatt tcatcgacgg taagaaagcc
    24061 tttattggtg acgccgctcg gtttgtagcc atccggcagg cagtaggtgc aacgaaagtt
    24121 acacacatcg gtaatcgaca ggcgcaagta gtaaaactta cgcgcaaatg catcagtcag
    24181 ttgtgaagcc atgtacacct ttccagatac gggaggcgaa gtcatttctt ccttcgccct
    24241 ggtggcaatc tttacgagca ttgtcacggc caaagcaccg tatcagttga cccaggtgca
    24301 gaggctagag tgtttagtgg ttaagccgat actagcgtgt aaatgagatt tttaccatcc
    24361 acactttcgc tatgcaatca tgtatatagc gtcatgatcg tgctatttcg ctacagaata
    24421 agggaaaatg gcagttttac tttgatatac atcatcaggg aggcatagcg tgcatcgtcc
    24481 tttagaggta gaagaaaata gtttgtatcc tcaatattgg caggttaatt gctgtttccc
    24541 cgcaatttgc gctactgtag cgcggataat ctgactccag gaaactatat gcgcaatcgt
    24601 acgctggctg atcttgatcg tgtcgttgct ctcggcggag ggcatggact gggacgcgtt
    24661 ctctcatcac tttcgtcttt gggttctcgt ttaacgggta tcgtcaccac cacggataat
    24721 ggtggctcga cggggcgtat tcgccgttca gaaggcggca ttgcctgggg cgatatgcgc
    24781 aactgcctca accaactgat aacggaaccg agcgtcgcct ccgcgatgtt tgaataccgt
    24841 tttggcggca atggcgaact ttccgggcac aaccttggaa acttgatgtt aaaggcgctg
    24901 gatcacctta gcgtgcggcc tctggaagcc atcaatttaa ttcgtaatct gctgaaagtg
    24961 gatacgcatt tgattccaat gtcagagcat cccgtcgatc tgatggcgat tgacgatcag
    25021 gggcatgaag tttacggcga ggtcaatatc gaccagttaa ctacgccgat tcaagagtta
    25081 ttattaacgc cgaatgtacc cgcaacgcgt gaggcagttc acgctatcaa tgaagcggat
    25141 ctcatcatta ttgggcctgg cagtttttat accagcctga tgccaattct gctgctgaag
    25201 gaaatcgccc aggcattacg ccgcacgcca gcgccgatgg tttatatcgg caatctgggg
    25261 cgtgagttga gtttacctgc ggctaatttg aagctggaaa gcaagctggc aattatggag
    25321 cagtatgttg gtaaaaaagt cattgatgcg gtcatcgtcg ggccaaaagt ggatgtctcg
    25381 gcggtgaaag agcggattgt gatccaggag gtactggagg ccagcgatat tccgtatcgt
    25441 catgaccgcc agttgttaca taacgcgctg gaaaaggcgt tacaggcttt aggttaacgg
    25501 aaatccgaag gaaaattccg gcttcctatt gaagacaaag tgcgcgttgt ttatgccgga
    25561 tgcggcgtga acgccttatc cggcctacaa accgcgcaaa ttcaatatat tgcggagaaa
    25621 atgtaggcct gataagcgta gcgcatcagg ctgttttccg tttgtcatca gtcttcttcg
    25681 ctatcctgtt acgatgcggc aataaacaac tcacgcaact gatgcaactg gtcacgaatt
    25741 tgtgccgctt cttcgaactc cagattctgc gcgtgttgca tcatcagccc ttccagttca
    25801 tggattttct gctgcaacgc tttcggcgac atatccatcg gcacattatc cggctcaaca
    25861 atcgggcgcg attttcctct gcccttcgct ttggttttgg caatgttctg ccccagcgcc
    25921 aggatatcga ccactttctt gttcaagcct tgcggcgtaa tgccgtgttc ctcgttgtac
    25981 ttctgctgtt tctcacggcg acgttcggtt tcgccaatcg ctttcgccat tgatggggtg
    26041 atcttatcgc cgtagagaat cgctttaccg ttaacgttac gtgccgcacg accaatggtc
    26101 tggatcaacg aacgttcgga acgcaggaag ccttctttgt cagcgtcgag gatcgccacc
    26161 agcgacactt ccggcatatc cagaccttcg cgcagtaagt tgatccctac cagcacgtcg
    26221 aactcaccca gacgcaagtc gcggataatc tccatacgtt cgacggtgtc gatatctgag
    26281 tgaagataac gcacgcgctc gccatgttct tcgagatatt cggtaagatc ttccgccatc
    26341 cgcttggtca gtgtggtgac cagtacgcgt tcgttaattg ccgctcgctg acgaatctcc
    26401 gaaagaagat catcaacctg tgtcgccacc ggccgcactt cgataatcgg gtcaagcaat
    26461 ccggttggac gcaccacctg atccaccaca tcgccgccgg atttttccag ctcgtaatta
    26521 cccggcgtcg ccgaaacata gatggtttgc ggcgctaatg cttcgaactc ttcaaactta
    26581 agcggacggt tatccagcgc tgatggcagg cggaagccgt actccaccag tgtctcttta
    26641 cgcgcccggt caccgcgata catgccgcca atttgtggaa tggtgacgtg agattcatcg
    26701 acgaccagca gcccatcggc aggcaggtaa tcaaacagcg tcggcggtgg ctcacccggt
    26761 ccacgaccgg agaggaagcg cgagtagttt tcaatccccg aacagtagcc cagctcgttc
    26821 atcatctcca gatcaaactg ggtacgctgg gtcagccgct gctcttccag cagtttgttg
    26881 ttttccaaca gcactttgcg tctggcggcc agttcttctt tgatctcctc catcgcctgt
    26941 acgatgcgct cgcgcggtgt gacgtaatgc gttttcgggt agatggtaaa acgtggaata
    27001 gtggaaacaa tctgcccggt cagcgggtca aataacgaca atcgttccac ttcctcgtca
    27061 aacagttcca cgcgaagtgc aatgtcatcc gattctgccg ggaagatatc aatcacctcg
    27121 ccacgaacgc ggaaagtacc acgctggaat gcttgatcat tacgagcgta ttgcagctcc
    27181 gccagtcggc gcagaatcgc gcgctgatcg ataatcatac cgaccgtgag atggagcatc
    27241 atcttgagat ataaatcagg atcgcccaga ccataaatcg cggaaacaga ggccactaca
    27301 accacatcac gccgctccag catcgctttg gtggcggata aacgcatctg ctcaatatgt
    27361 tcgttaaccg aggcatcttt ctcaatgaaa gtgtcggaac tcggtacata ggcttccggc
    27421 tgatagtagt cgtagtagga gacgaaatac tccaccgcgt tttccgggaa gaactctttc
    27481 atttcgccat acagctgggc cgccagcgtt ttgttgggcg caagtaccat ggttgggcgc
    27541 tgaaggtcag caatgacatt ggcaatggtg aaggttttcc ctgagccagt cacgccaagt
    27601 aacgtctggt gcgccaggcc atcttccagc ccctcttcga gacgtcgaat cgcctctggc
    27661 tgatcgccag aaggtttaaa agcggaattc agtttgaacg gtttactcat gagtcgctac
    27721 ctgaaggagt tgggcgggca ggtatgtaat tttactcgtc gtacttaatt atgccaacaa
    27781 attatactgg ataaaaaaac agttcatcac cataatattt ctgatacagc gtaaactccg
    27841 ctgtcaatca tgagcaaaat ttactctgtg gcgagataaa actccggcct taccgggtta
    27901 tccccaaatc aacggctttt ttaacatttg tcaagatgag tgatgacagt tttatggcaa
    27961 gagatgcctg ttcagtgact gccattgcat ttatctaacc agttaaaaaa taaaagatat
    28021 ttctttgagc cgtctttaac gccaattcgc gtgcaagccg cgtattctct cgcttgcctc
    28081 gtgttttcta actctaatac acatggttat ccacaggaat agtggataac tgtctccagc
    28141 ccctatcccc cgccgcctgg gcaattccca cattcgccgc atgaggcgga agtaaaattt
    28201 ttttcgtgat tattttagaa tttaattggt taaattgcag tcaatcgaag acgcgatctc
    28261 gctcgcaatt taaccaaata cagaatggct acaacaaggc aaggtttatg tactttccgg
    28321 ttgccgcatt ttctggattt tctgcaagcc aggggatctc tcccagcagc ggcgcgggaa
    28381 tcatgcgggt gagcgtggtc atatattcag cgtgacgttt tcccggaggc gtaacatcgt
    28441 tcgccaccca acccgccaga gtcagtccgg cgtattgtat tgcctgcgca gtcaacatcg
    28501 cgtgattaat acagccgagt ttcacaccaa ctaccagtat caccggcagt tgttcctgtg
    28561 ttacccaatc tgcaaaagtg aaagtgtcag aaagcggcgt aaaccagccg ccagcacctt
    28621 ccactaacac ccagtcagcc tgttgttcaa gcgcgcgtaa tccggcgctc attaccaatg
    28681 attctatcgg cctgccctct tgcgcgctga tgatgtgcgg cgaagtgggt tctgcgaagg
    28741 tgtaaggatt tactgttgcg taatccagct gcaggctgct gttgtgctgt aacgccagcg
    28801 cgtcactatt acgtaagcct tccggggtct tttcgctgcc agaggcgacc ggtttataac
    28861 ctgccgtccg ataacctact gccttcgcgg cttgtaaaag tgcacaactg gcgacggttt
    28921 tccccacttc ggtatccgtt ccggtgacaa aataacgttt actcacgagc aatcactccc
    28981 aaaaaaagat gatacgtcag aggatatcgc ccctgttgtt gcggccaggc cagttgcaat
    29041 cgctgcaatt gcgaacgcgt taatattcgc gggtcgcgcc cttcatgaag atgcgtagca
    29101 ccgatgcctt tcagcgaacg catggcactg agcgcatcat caaaccatag tgtgatgggc
    29161 tgaatatgat gttgataatg cacgccgttc agcgactgtt cgatttcatc tggcggtaaa
    29221 aagcgattag catgcggacg ctcgtccacc gcctgccacg cctgatgcag ttcgggtaac
    29281 gatccctgca ccagcgtggt aaacgccacc acgcctccgg ggcgcaccac ccgatacagc
    29341 tcgcggagtg ccgtggataa attaccgcac cactgcaccg cgagattgct ccatgcaaga
    29401 tcgaacgtcg cagtcgctaa cggcagggat tcgatatctc ccgccagata atggtctgca
    29461 gcatctttct ggcgggcttg cacaagcatt ggcggtgaga gatctaacgc cgtcacctgt
    29521 gcgtgacgct ctcgccagtg gcggctcatc cagcctggcc cacaccccgc gtccagtacg
    29581 tgggtgtatt tacgctgtgg gagcattgcc agtaagacgt cagcactccg gcgctgtaac
    29641 tctgcgtgtt gctcatagtg cgcggctgcc cgaccaaatg ccgctgcaat ggcttgttta
    29701 ttaaccgttg ccatgcagca cctccagcag acggtcgata tcctgcattt catgcgctgc
    29761 ggttagcgtt aagcgcagtc gcgcagtacc agcgggtacg gttggcgggc gaatcgccgt
    29821 gacccagcag ccttgctggc gcagtttttc tgccagttgt aacgcacggc tgttatcacc
    29881 gacgatcaat ggctggatgg cgctgcatga atcagcaagc gtaaacggca aatcctgtac
    29941 tccggcacga aaacgcgcaa ttagtgacac cagtttttcg cgccgtgcat ccccctcatc
    30001 acggcgaatg accgccagcg acgcacgtaa tgcctgcgcc tgagcgggcg gcatactggt
    30061 gttgtagata aggtggcggg cgaattgcag cagataatcc gccaccgtac tggagcaaag
    30121 caccgctgcc ccgctgacgc caaatccttt gccaaaagtc accaccagca attctggttt
    30181 taccttttgc agccagcagc tgccgcgccc ctgctccccg ataacgcccg tgccgtgggc
    30241 atcatcgacc atcaaccagc cattgtgctg ttgcgttacc tgctggattt ccgccagtgg
    30301 cgcactatcg ccgtccatgc tgaacacgcc ttctgtcacc accagttgct gccccggaca
    30361 gggggaagca agcagtcgcg ccagatgagt gacatcgtta tgaacaaaac ggcgaagctg
    30421 cgacgggctt aaactggcag cctccagcaa tgaggcatgg ctaagccggt cagcgacaat
    30481 gcggtcctct tttgccatca tcgcggcaat gaccgcctga ttagcggcaa aaccagagat
    30541 aaatagcagt gcccgcgaat agcccagcca ctcggccagt tcttcttcca gcgcctgatg
    30601 cgcaacgcta taaccgctga cgtgaccgga accgccgcta ccgacgccaa attgctccgc
    30661 accctgctgc caggcacgga taatttgcgg atgatggctt aaacctaaat aatcgttact
    30721 ggaaaagttc agatactggc gatcatccgc caccagccag cgtccggctc cttgcgccac
    30781 tggataacgg cgacgcaggg catcggcagc acgccgcgca tcgagcgccg cgttgatttt
    30841 ctcctgccag ctcatagtgc tgccgcgttg taatattcgt cagtatccgg ggtcatcagc
    30901 gcctgttcaa gacgttgctg ttgttcgtta tcccctgcca gcacggcagt ttgctgcgga
    30961 tttagcccca gtttgcggaa cagttgcagg tctttatctt cttccggatt cggcgtggtc
    31021 agcagtttgc aaccgtagaa aatcgagttt gcgcctgcca taaagcacat cgcctgagtc
    31081 tgttcgttca tctgctcgcg tccggcagaa aggcgcacgt aagaggtcgg catcatgatc
    31141 cgcgcgaccg caatggtgcg aataaaatca aaggcatcaa cgtcatcgtt atcggcaagc
    31201 ggcgtgcctt tcaccttcac cagcatgttg attggcacgc tttccggcgg cgtcggcagg
    31261 tttgccagtt gcagcaataa tccggcgcga tcttttaccg tttcgcctaa gcccacaatg
    31321 ccgccagaac agactttgat cccggcatcg cgcacttttt ccagcgtatc gaggcgttcc
    31381 tgataagtgc gggtggtgat gatattgccg taaaactccg gcgaggtgtc gaggttgtgg
    31441 ttgtagtaat ccagcccggc gttcgcgagg cgctgcgcct gagattcact caacgtgccc
    31501 agcgtcatac acgcctccag ccctaaatct tttaccccat gcaccatctg ctccaggtac
    31561 ggcatatcgc gttcgttggg attcttccac gccgcgccca tgcaaaaacg cgtcgatccc
    31621 gccgctttcg ctttgcgcgc cgactccagc acctgttcaa cttccatcaa ccgctcggct
    31681 tccagcccgg ttttatagcg cgagctttgc gggcaatatt tgcaatcttc cgggcaagca
    31741 ccggttttaa tcgacagcaa cgtgctgacc tgcacctgac gaggatcgaa atgctggcga
    31801 tgcacctgct gcgcttcaaa cagcagatcc agcaacggtt tttcaaataa ttctgcgact
    31861 tgcgacaatg tccagcgtgg gcggtgagcc atggggcttc tccaaaacgt gttttttgtt
    31921 gttaattcgg tgtagatttg taaacctaaa tcttttcaat ttggtttaca agtcgattat
    31981 gacaaaggac gatcttgcct ttgaccaacg ccatatctgg cacccataca catccatgac
    32041 ctccccgctg ccggtttatc cggtggtgag cgccgaaggt tgcgagctga ttttgtctga
    32101 cggcagacgc ctgattgacg gtatgtcgtc ctggtgggcg gcgatccacg gctacaatca
    32161 cccgcagctt aatgcggcga tgaagtcgca aattgatgcc atgtcgcatg tgatgtttgg
    32221 cggtatcacc catgcgccag ccattgagct gtgccgcaaa ctggtggcga tgacgccgca
    32281 accactggaa tgcgtttttc tcgcggactc cggttccgta gcggtggaag tggcgatgaa
    32341 aatggcgttg cagtactggc aagccaaagg caaagtgcgc cagcgttttc tgaccttccg
    32401 caatggttat catggcgata cctttggcgc gatgtcggtg tgcgatccgg acaactcaat
    32461 gcacaacctg tggaaaggct atctgccaga aaacctgttt gctcccgccc cgcaaagccg
    32521 catggatggt gaatgggatg aacgcgatat ggttggcttt gcccgcctga tggcggcgca
    32581 tcgtcatgaa atcgcggcgg tgatcattga accgattgtc cagggcgcag gcgggatgcg
    32641 catgtaccat ccggaatggt taaaacgaat ccgcaaaatg tgcgatcgcg aaggtatctt
    32701 gctgattgcc gacgagatcg ccaccggatt tggtcgtacc ggcaaactgt ttgcctgtga
    32761 atatgcagaa atcgcgccgg acattttgtg cctcggtaaa gccttaaccg gcggcacaat
    32821 gaccctttcc gccacactca ccacgcgcga ggttgcagaa accatcagta acggcgaagc
    32881 cggctgcttt atgcacgggc caacttttat gggcaatccg ctggcctgcg cggctgcaaa
    32941 cgccagcctg gcgattctcg aatctggcga ctggcagcaa caggtggcgg ctattgaagt
    33001 acagctgcgc gagcaacttg cccccgcccg tgatgccgaa atggttgccg atgtgcgcgt
    33061 gctgggggca atcggcgtag tcgaaacaac tcgtccggtg aatatggcgg cgctgcaaaa
    33121 attctttgtc gaacagggag tatggatccg gccttttggc aaactgattt acctgatgcc
    33181 gccctatatt attctcccgc aacagttgca gcgtctgacc gcagcggtta accgtgcggt
    33241 gcaggatgaa acattttttt gccaataacg ggaagtcagc gtgtgggttt ctggctacac
    33301 tttctgcaaa ctagaaagga gggttcatga aactcatcag taatgatctg cgcgatggcg
    33361 ataagctgcc gcatcgtcat gtctttaacg gcatgggtta cgatggcgat aatatttcac
    33421 cgcatctggc gtgggatgat gttcctgcgg gaacgaaaag ttttgttgtc acctgctacg
    33481 acccggatgc gccaaccggc tccggctggt ggcactgggt agttgttaat ttacccgctg
    33541 atacccgcgt attaccgcaa gggtttggct ctggtctggt agcaatgcct gacggcgttt
    33601 tgcagacgcg taccgatttt ggtaaaaccg gatacgatgg cgcagcgccg ccgaaaggcg
    33661 aaacccatcg ctacattttt accgttcacg cgctggatgt agagcatatt gatgtcgatg
    33721 aaggagccag cggcgcgatg gtcgggttta acgttcattt ccactctctg gcaagcgcat
    33781 cgattaccgc gatgtttagt taatcactct gccagatggc gcaatgctat ctggtatcac
    33841 ttaaaggtat taaaaacaac tttttgtctt tttaccttcc cgtttcgctc aagttagtat
    33901 aaaaaagcag gcttcaacgg attcattttt ctatttcata gcccggagca acctgtgaac
    33961 acattttcag tttcccgtct ggcgctggca ttggcttttg gcgtgacgct gaccgcctgt
    34021 agctcaacac cacccgatca acgtccttct gatcaaactg cgcctggtac ctcttctcgc
    34081 ccgattctgt cggcaaaaga agcgcagaat ttcgatgctc aacactattt tgcatccctg
    34141 acaccaggtg ctgcagcgtg gaatccttcc ccgattaccc tgcctgcgca acctgacttt
    34201 gttgtcggcc cggcgggcac tcaaggtgta acgcatacca cgattcaggc ggcggtagat
    34261 gcggcaatta tcaagcgtac caacaagcgc cagtatattg ccgtgatgcc tggtgagtat
    34321 cagggaacgg tgtatgtccc tgctgctccg ggtggaatta ctctgtacgg tacaggtgaa
    34381 aaaccgattg atgtgaagat tgggctttcc cttgatgggg gcatgagccc tgccgactgg
    34441 cgtcacgacg tcaacccgcg cggcaaatat atgccaggta aaccagcgtg gtatatgtac
    34501 gatagctgcc agagcaaacg cagcgacagt atcggtgttc tctgctctgc ggtcttctgg
    34561 tcacaaaaca atggcctgca actgcaaaac ctgaccatcg aaaacacgct gggcgatagc
    34621 gtagatgcag gtaaccatcc ggcggtggca ctgcgtactg atggtgacca ggtacagatt
    34681 aacaacgtta acattctcgg tcgtcagaac accttctttg tcaccaacag cggtgtgcag
    34741 aaccgtctgg aaacgaatcg tcagccgcgt acgctggtga ccaacagcta cattgaaggg
    34801 gatgtggata tcgtttctgg tcgcggcgca gtggtgttcg ataacaccga attccgcgtg
    34861 gtgaactcac gtactcagca agaagcgtat gtgtttgcac cggctacgct gtccaacatt
    34921 tactacggtt tcctcgccgt aaacagccgt ttcaatgctt tcggtgatgg tgtggcgcaa
    34981 ctgggccgct cgctggatgt tgatgccaat accaacggtc aggtggtgat ccgtgatagc
    35041 gccatcaacg aaggttttaa cacggctaaa ccgtgggccg atgcggtgat ctctaatcgt
    35101 ccgtttgcgg gtaataccgg cagcgtagat gataacgacg aaatacagcg caatctgaat
    35161 gacactaact acaaccgcat gtgggaatac aataaccgcg gcgtgggtag taaagtggtt
    35221 gcagaggcga agaagtaaga gcaattaact atttgccgga tgcggcgtaa acgccttatc
    35281 cggcctacgg ttcgatgcga tttgtaggtc ggataagatg tgcaagcatc gcatccgaca
    35341 ataagtgccg aatgcgacct acattcacat ggcgcttttt acatctgacg gtttttattg
    35401 aagttaatca aactacccgc cttgataatc tcgcgctctt cagcagtcag actttccata
    35461 cagagcgtaa tttccgttac cggcgcatct tcatggatca cataaccttt aaacgtcgta
    35521 cccggattat ccagcgccgc tttaatgcca gggatgtaaa tgtaatcccc cacttcaaag
    35581 gttggcactt ccgccatctg cagcggtaac atcccccagt tgatgacgtt agaacggtag
    35641 cgtttagtcg cgtactcttc ggcaatattc gccagaccgc caatcacacg ctggcagctc
    35701 gccgcctgtt cacgcgcaga accatcgcct ggtttcaccg catataccat gctgccaatt
    35761 tcagtttgca gcggatcaat atgctcctga ccagcaatct gcttaatgcg cgcaaacacc
    35821 tctgtcagct cgctgacatt ccccgccaga cgctgatttt ccagctcagc agtcgcttta
    35881 cttctgccaa cataaccggg atcgcggcgt gacagggtaa actccgccag accaatcgga
    35941 tttgaacgat aagaagaggt ttcaccggaa ggaatcagtt cgtcggtggt ggtcacttcg
    36001 tcgaggatct tcgagcacac tttcaggacg atattgtcag tcagcgcacc caattccggc
    36061 cagtctttaa tgttcggtcc gtaaatcagc ggttgctgag ttgccccttt cacaaagccc
    36121 tgataaacac ggtttttata cggcgttaca tcgaaggcgt actccggcac gttgtcccag
    36181 caatcaagtt cgctggcaga ggttaaatag ccaccgtttg ccgcagtcgc agcgatagaa
    36241 cgagcgtcca tcaacgccac cgctgacatc tgcccattag ctggcttaga gccttcgcgg
    36301 ttcgggaagt tacgcgtggt gtggcgaata cttaatccgt tgttgattgg cgtatcgccc
    36361 gcgccaaagc atgggccgca gaacgcggtt ctgatgattg cgcctgcgcc aatcaaatct
    36421 gctaccacac cttttttggc taaatccata aacaccggct gtgatgacgg gtaaaccgcc
    36481 agcgagaagg tgtcattgcc acaggattga ccgcgcagtg cattcgccgc cgcgatgacg
    36541 ttttcgtagt taccgccaga acagcccgcg ataatcccct gctgcacttt caggcgacca
    36601 ttttccactt tatccagcag cgagagtttg gctttaccgt gcgccacgcg ttcggactca
    36661 atttcaatct cacgcagaat gtcggtcagg ttctggttca gtgtgtcgat ttcatacacg
    36721 ttgctcgggt ggaacggcag cgcaatcatt ggtttgatgg cgcttaaatc aacgctgatg
    36781 cagccatcgt agtacgccat cggttgaggg ttaagctggc agtaatcctg gccgcgaccg
    36841 tgcagcgcca gccagttatg cacttcttca tcagtttgcc agacggagct taaacaggtg
    36901 gtttccgtgg tcatcacgtc aacgctgtta cggaaatcgg tagagagcgc gctaacgccc
    36961 ggtccaacga actccatgac tttgtttttg acgtaaccgt ttttgaacac cgcgccaatg
    37021 atagccagcg ccacatcctg tggccccaca tacggcgcag gttttccggt cagatgcacc
    37081 gcaaccacgc ccggatagtc gatatcctag gtgtcattaa gcagctgttt taccaactca
    37141 ccgccgccct caccgactgc cattgtccct aatgcaccgt aacgggtgtg gctgtctgac
    37201 ccgaggatca ttttgccccc gcctgccatc atctcacgca tatattgatg gatgaccgca
    37261 atatgcggag gcacaaaaat accgccataa cgctgagctg ccgataaacc aaaaacatgg
    37321 tcatcaccgt taatggtgcc gccgacggcg cagagtgagt tatggcagtt ggtcagcaca
    37381 tacggcagcg ggaaacgttc cattccggac gctttagcgg tctgtacaat accaacaaag
    37441 gtaatatcgt gagaggctaa tgaatcaaac ttaattttaa gtttatccat atttccggac
    37501 gtattatgag aagagagaat agaccaggca atagtgcctt ttttggcttc ttctttttta
    37561 atttcgccgg tgaaatgttc ttcggcaatt atttcgttat tactggcgag aaacacgcct
    37621 ttttcaggta atga
//
LOCUS       DAJTNA010000017        35261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTNA010000017 DAJTNA010000000
VERSION     DAJTNA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35261
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.017"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..181
                     /locus_tag="OO161_000741"
     CDS             <1..181
                     /locus_tag="OO161_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5461908.1"
                     /translation="TRSASQSLNIPSEIKEGQTTDWININSDNDNKRCVSKITFSGHT
                     VNSSDMATLKIIGDD"
     gene            207..920
                     /gene="msyB"
                     /locus_tag="OO161_000742"
     CDS             207..920
                     /gene="msyB"
                     /locus_tag="OO161_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5461909.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIPCLRRMVSATQV"
     gene            1069..1635
                     /gene="satP"
                     /locus_tag="OO161_000743"
     CDS             1069..1635
                     /gene="satP"
                     /locus_tag="OO161_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5461910.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(1670..2257)
                     /gene="mog"
                     /locus_tag="OO161_000744"
     CDS             complement(1670..2257)
                     /gene="mog"
                     /locus_tag="OO161_000744"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5461911.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2372..3325)
                     /gene="tal"
                     /locus_tag="OO161_000745"
     CDS             complement(2372..3325)
                     /gene="tal"
                     /locus_tag="OO161_000745"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5461912.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3604..5028
                     /locus_tag="OO161_000746"
     CDS             3604..5028
                     /locus_tag="OO161_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCR5461913.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQLI
                     QKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRICTF
                     ATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPV
                     FDPLRYPDIGRQLSPDAWDDVSQE"
     gene            5098..5874
                     /gene="yaaA"
                     /locus_tag="OO161_000747"
     CDS             5098..5874
                     /gene="yaaA"
                     /locus_tag="OO161_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5461914.1"
                     /translation="MLILISPAKTLDYQGPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(6027..6323)
                     /locus_tag="OO161_000748"
     CDS             complement(6027..6323)
                     /locus_tag="OO161_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5461915.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGLGKHHR"
     gene            complement(6537..7823)
                     /gene="thrC"
                     /locus_tag="OO161_000749"
     CDS             complement(6537..7823)
                     /gene="thrC"
                     /locus_tag="OO161_000749"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5461916.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(7824..8756)
                     /gene="thrB"
                     /locus_tag="OO161_000750"
     CDS             complement(7824..8756)
                     /gene="thrB"
                     /locus_tag="OO161_000750"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5461917.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(8758..11220)
                     /gene="thrA"
                     /locus_tag="OO161_000751"
     CDS             complement(8758..11220)
                     /gene="thrA"
                     /locus_tag="OO161_000751"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5461918.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(11246..11365)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(11301..11366)
                     /gene="thrL"
                     /locus_tag="OO161_000752"
     CDS             complement(11301..11366)
                     /gene="thrL"
                     /locus_tag="OO161_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5461919.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(11580..12266)
                     /locus_tag="OO161_000753"
     CDS             complement(11580..12266)
                     /locus_tag="OO161_000753"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5461920.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(12666..12806)
                     /locus_tag="OO161_000754"
     CDS             complement(12666..12806)
                     /locus_tag="OO161_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461921.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            12902..13618
                     /gene="arcA"
                     /locus_tag="OO161_000755"
     CDS             12902..13618
                     /gene="arcA"
                     /locus_tag="OO161_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5461922.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(13678..14976)
                     /gene="creD"
                     /locus_tag="OO161_000756"
     CDS             complement(13678..14976)
                     /gene="creD"
                     /locus_tag="OO161_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5461923.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLGTSVL
                     VVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK"
     gene            complement(15034..16458)
                     /gene="creC"
                     /locus_tag="OO161_000757"
     CDS             complement(15034..16458)
                     /gene="creC"
                     /locus_tag="OO161_000757"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5461924.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLYDSANKAVGQDYSRWNDVWLTLHGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVVAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(16458..17147)
                     /gene="creB"
                     /locus_tag="OO161_000758"
     CDS             complement(16458..17147)
                     /gene="creB"
                     /locus_tag="OO161_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5461925.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(17160..17633)
                     /gene="creA"
                     /locus_tag="OO161_000759"
     CDS             complement(17160..17633)
                     /gene="creA"
                     /locus_tag="OO161_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5461926.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMVGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            17844..18713
                     /gene="robA"
                     /locus_tag="OO161_000760"
     CDS             17844..18713
                     /gene="robA"
                     /locus_tag="OO161_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5461927.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(18710..19357)
                     /gene="gpmB"
                     /locus_tag="OO161_000761"
     CDS             complement(18710..19357)
                     /gene="gpmB"
                     /locus_tag="OO161_000761"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5461928.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTQRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSGRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            19409..19930
                     /gene="yjjX"
                     /locus_tag="OO161_000762"
     CDS             19409..19930
                     /gene="yjjX"
                     /locus_tag="OO161_000762"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR5461929.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(20015..20341)
                     /gene="trpR"
                     /locus_tag="OO161_000763"
     CDS             complement(20015..20341)
                     /gene="trpR"
                     /locus_tag="OO161_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5461930.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(20431..22368)
                     /gene="sltY"
                     /locus_tag="OO161_000764"
     CDS             complement(20431..22368)
                     /gene="sltY"
                     /locus_tag="OO161_000764"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5461931.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATSAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            22579..24246
                     /gene="ettA"
                     /locus_tag="OO161_000765"
     CDS             22579..24246
                     /gene="ettA"
                     /locus_tag="OO161_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5461932.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(24553..25785)
                     /gene="nadR"
                     /locus_tag="OO161_000766"
     CDS             complement(24553..25785)
                     /gene="nadR"
                     /locus_tag="OO161_000766"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5461933.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(25806..27188)
                     /gene="radA"
                     /locus_tag="OO161_000767"
     CDS             complement(25806..27188)
                     /gene="radA"
                     /locus_tag="OO161_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5461934.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(27237..28205)
                     /gene="serB"
                     /locus_tag="OO161_000768"
     CDS             complement(27237..28205)
                     /gene="serB"
                     /locus_tag="OO161_000768"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5461935.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            28311..28955
                     /locus_tag="OO161_000769"
     CDS             28311..28955
                     /locus_tag="OO161_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5461936.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGTSWFSQNHQRQRNPQ
                     LEELTRPLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTLWQQSPFLLTASKPVPEEEESEKKE"
     gene            28983..29999
                     /gene="lplA"
                     /locus_tag="OO161_000770"
     CDS             28983..29999
                     /gene="lplA"
                     /locus_tag="OO161_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR5461937.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            30031..30180
                     /locus_tag="OO161_000771"
     CDS             30031..30180
                     /locus_tag="OO161_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5461938.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(30455..31174)
                     /gene="deoD"
                     /locus_tag="OO161_000772"
     CDS             complement(30455..31174)
                     /gene="deoD"
                     /locus_tag="OO161_000772"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5461939.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(31254..32477)
                     /gene="deoB"
                     /locus_tag="OO161_000773"
     CDS             complement(31254..32477)
                     /gene="deoB"
                     /locus_tag="OO161_000773"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5461940.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(32529..33851)
                     /gene="deoA"
                     /locus_tag="OO161_000774"
     CDS             complement(32529..33851)
                     /gene="deoA"
                     /locus_tag="OO161_000774"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5461941.1"
                     /translation="MFLAQEIIRKKRDGHALNDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(33978..34757)
                     /gene="deoC"
                     /locus_tag="OO161_000775"
     CDS             complement(33978..34757)
                     /gene="deoC"
                     /locus_tag="OO161_000775"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5461942.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            35015..>35261
                     /locus_tag="OO161_000776"
     CDS             35015..>35261
                     /locus_tag="OO161_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HCR5461943.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAN"
BASE COUNT         8357 a         9598 c         8826 g         8480 t
ORIGIN      
        1 cacccgtagc gccagtcaga gtctgaatat tccttcagaa ataaaagaag ggcaaaccac
       61 tgactggatc aacattaaca gtgataacga caataaacgc tgcgtctcaa aaatcacctt
      121 ttccggtcat acggtgaact catcggatat ggccacgctg aaaattatcg gcgacgacta
      181 acgccagaat atagaagcca caaaaaatga atgttaatta cctgaatgat tcagatctgg
      241 attttcttca gcattgtagt gaggaacagt tggcaaattt cgcccgattg ctcacccata
      301 atgaaaaagg caaaactcgc ctctccagcg tactgatgcg caacgaactg tttaaatcga
      361 tggaagggca tcccgagcaa catcgccgca actggcagct gattgctgga gaattacagc
      421 attttggtgg cgatagtatc gccaacaaac tgcgcggaca cggtaaattg tatcgggcca
      481 ttttgctcga tgtttcaaaa cgattgaagc tgaaagccga caaagagatg tctacgtttg
      541 aaattgagca acagttactg gaacaatttc tgcgtaatac ctggaagaaa atggacgagg
      601 aacataagca ggagtttctg cacgcgttcg atgcgagggt gaatgagctg gaagagctgc
      661 tgccgctgct gatgaaagac aaattattgg caaaaggcgt gtcgcatttg ctttccagcc
      721 aactgacccg cattttacgc acccacgcag caatgagcgt acttgggcat ggtttgctgc
      781 gcggcgcggg gctgggaggc cctgtaggtg cggcactaaa tggggttaaa gcggtcagcg
      841 gcagcgccta tcgcgtgacg attccagccg tactgcaaat cccctgcctg cgccggatgg
      901 ttagcgccac tcaggtctga taccggaaac cggggaaaat tttcattttc ccctcaaaag
      961 atcgtagaca ctgccccact ggctgattat tatgccgcgc cctgaaaaca ctacagttat
     1021 tcagggaaat tatttcacca ttcattcgat gatgattttt gaggaattat gggcaacact
     1081 aagttggcta atccggcacc gctgggcctg atgggcttcg gcatgaccac cattctgctt
     1141 aacctgcaca acgtgggtta tttcgctctg gacggtatta ttcttgccat gggcattttc
     1201 tacagcggca tcgcgcaaat ttttgctggt ctgctggagt acaaaaaagg caacactttc
     1261 ggtttaaccg cattcacctc ttacggttct ttctggctga cgctggttgc gattctgctg
     1321 atgccgaaac tgggtctgac cgatgcgcca aatgcacagt tccttggtgt ctacctgggt
     1381 ctgtggggcg tatttacgct gtttatgttc ttcggcacgc tgaaaggcgc acgcgttctg
     1441 caattcgttt tctttagcct gactgtgctg tttgccctgc tggcgatcgg taacattgcc
     1501 ggtaacgccg caatcatcca ctttgccggc tggattggtc tgatctgtgg tgccagtgca
     1561 atctatctgg cgatgggtga agtactgaac gagcagtttg gtcgcaccgt tctgccgatt
     1621 ggtgaatccc actaattgtt ttgagatccc cccgctcggg gggatttttt tattcgctaa
     1681 cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac ttctggtgcc gtttcaacgt
     1741 atggcccttc cagcaactga atgcagtacg gtacgctggc aaaaataccg tgtaccacaa
     1801 cgttacccgc agcgtccttc acaccttcca gcgtctcttt aatagacttc ggctgaccgg
     1861 gtaagttaag gatcagcgcc tgtttgcgaa tcacaccaac ctgacgcgaa aggatcgcag
     1921 ttggtacaaa atgcaggctt atctggcgca tctgttcacc aaagccaggc atctcgcggt
     1981 ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg cgccgggcca gttccgcccg
     2041 tggtgagcac cagatggcaa ctcatttcat ccactagctc acacaacgtt tgctcgatga
     2101 tcgcctgctc atcggggatt aagcgggttt ccagttcaaa cggcgtggtt agcgccgatg
     2161 tcagccattc ttccagcgca gggatgcctt tatcctgata aacgccgctg gatgcgcgat
     2221 cagagatgga aactaagcca atacgtaaag tattcatcaa gatttttccg tttaaacagt
     2281 ctcgttaaac agaatgatac actgcgaagg gagtgacaga caggcttcag aagaggtagc
     2341 gtgaccgact tcccggtcac gctaagaatg attacagcag atcgccgatc attttttcca
     2401 gtttttcctg gtcaatagca aacttacgga taccttccgc cagtttatct actgccattg
     2461 gatcctggtt gtgctgccac aggaactcgg actcagtgat acgcgccgga cgcgctttca
     2521 cttcgccggt gtaagacagt ttacgttcga tagccccttc gctctcagcc agctctttca
     2581 gcagtgtagg tgcgatggta agacggtcgc agcctgccag ttccagaatt tcgccgatgt
     2641 tacggaagct tgcgcccata accacggttt cataaccgtg ctctttgtag tactggtaga
     2701 tttcagatac agaaaccacg cccggatctt ctgccggagc gtactctttc ttatcagtat
     2761 tcgctttgta ccagtcaaga atacggccaa caaatggcga gatcaggaac acgcctgctt
     2821 ccgcacaagc acgagcctga gcgaaggaga acagcagggt caggttacag ttgatgcctt
     2881 ctttttccag ctgttctgca gcacggatac cctgccaggt agaagccagt ttgatcagaa
     2941 tacgatcgtt gctaatacca gcatcgttgt agagtttgat caggcgtttt gctttcgcaa
     3001 ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc agttgagata cgacccggaa
     3061 ccagtttcag gatttccaga ccaatattta ctgccagttt gtcggtcgcg tccacgatct
     3121 gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac agcatcatca atcaacttac
     3181 ggtattccgg aatctgcgct gcgttaagaa tgagagaagg gttggttgtg gcatcctgcg
     3241 gttgatacag cttcattgcc gcgatgtccc cagtgtcggc cactacggtg gtgtactgac
     3301 gaagggaggt caatttgtcc gtcatgatag tatttctctt taaacagctt gttaggggga
     3361 tgtaaccggt ctgccctgat gatatcacga cgcaacgtca gcgcaaccgc cgacaagacg
     3421 gttatcgcca ggcgagactg tttcggattt ctgacactgc gttgatatcg cccgccattt
     3481 ttatacaaaa cctcatgtat gctacgcaga agttatcaag tacctcgtag cgtatatact
     3541 tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc gaccggcaat aagagggata
     3601 tgcatgccag attttttctc ctttattaac agcgtccttt ggggatcggt aatgatttac
     3661 ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg gatttgtgca gtttcgctac
     3721 atccgccagt ttggcaaaag tcttaaaaat agcattcatc cacagccagg cggtttaacc
     3781 tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg gtagcggcaa cctggccggc
     3841 gttgcgctgg ctattaccgc cggtggacct agagccgtct tttggatgtg ggttgccgcg
     3901 tttatcggca tggcgacctc gtttgccgaa tgttcccttg cacaacttta taaagaacgt
     3961 gacgtcaatg ggcagtttcg tggcggaccg gcatggtata tggcgcgcgg gctggggatg
     4021 cgttggatgg gcgttctgtt cgccgtcttt ttgctcatcg cctacggcat aattttcagc
     4081 ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt cttttgattt tcccccgctg
     4141 gtgacaggca ttattctcgc tgtctttgct ctgctggcaa tcactcgcgg tcttcatggc
     4201 gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt gctgaccagc
     4261 atggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc tatttttgag
     4321 agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt aagccaggcg
     4381 attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg ttcgacgcca
     4441 aacgcggcag cggcgtcctg gcctccgcat ccggcagcgc aagggattgt ccagatgatt
     4501 ggcattttta tcgacaccct ggtcatctgt acggcaagcg ccatgctgat attactggcg
     4561 ggtaacgaca caacctacat gccgctggaa ggtattcagc ttatccagaa ggcgatgcgg
     4621 gtgctaatgg gttcctgggg tgctgagttt gttaccctcg tggttattct gtttgccttc
     4681 agctccatcg ttgccaacta catttatgcc gaaaacaatc tcttcttttt acgcctgaac
     4741 acccctaaag cgatctggtg tttgcggatc tgcaccttcg caacggtcat cggcggcacc
     4801 ttgctaagcc ttccgctgat gtggcaactg gcagatatca taatggcctg catggctatt
     4861 accaatttga ccgccatttt actgctctcg cctgtggttc ataccattgc cagtgattat
     4921 ctacgccagc gtaaactcgg cgtgcgcccg gtgtttgatc cgttgcgtta tccggatatc
     4981 ggtcgccagc tttctccgga cgcgtgggat gatgtttcgc aggagtaatc acaactatcg
     5041 atcaactcat tctctttttt tgctaaagtc ggcataaatt tcctgcaagg actggatatg
     5101 ctgattctta tttcacctgc gaaaacgctt gattaccaag gcccgttgac caccacgcgc
     5161 tatacgctgc cggagctgtt agacaattcc cagcagttga tccatgaagc gcggaaactg
     5221 acgcctccgc agattagcac gctgatgcgc atcagcgaca aactggcggg tatcaacgcc
     5281 gctcgctttc atgactggca gccagatttc acgccggcga atgcccgcca ggcgattctg
     5341 gcgtttaaag gtgatgtcta caccggcttg caggctgaaa ccttcagcga agacgatttc
     5401 gattttgccc aacagcattt gcgaatgctt tccggcttgt atggcgtact gcgcccgctc
     5461 gatttaatgc agccttatcg tctggaaatg gggatccgtc ttgagaatgc ccgagggaaa
     5521 gatctgtatc aattctgggg agatatcatc accaacaagc tgaacgaggc gctcgcagca
     5581 caaggcgata atgtggtgat taatctggcg tcagatgagt attttaaatc ggtgaagccg
     5641 aagaaattga atgccgagat tatcaagccg gtgttcctcg atgagaagaa cggcaaattt
     5701 aagatcatca gcttctacgc taagaaagca cgcggtctga tgagtcgttt cattattgaa
     5761 aatcggctga ccaaaccaga gcaactgact ggttttaata gcgaaggtta cttctttgat
     5821 gaagcttcct ccagcaatgg cgaactggtg tttaaacgct acgagcagcg ttaagtttgc
     5881 agtcaatgcc ggatgcggcg tgaacgcctt atccggccta caaaagcatg cagattcaat
     5941 atattgcaga attcacgtag gcctgataag acgcgccagc gtcgcatcag gcgctgaatg
     6001 ccggattgtt acccggcatt tgtcatttag cggtgatgtt tgcctagacc atgaccgccg
     6061 tgatgatcat gaggagcttt cttatggtgg cgcggcggtg gcggcggtcc gtgtgggtgc
     6121 cagcgattgc ctcgccattc ataatgttgt ttccaccagc cgtggtcgcg ccagtgacct
     6181 ccatcccagt aatagccacg attatcacga tcgcctatct gtaattttac tgacgggact
     6241 aacgtaattt ccgcagcctg tgctgccatg ggagcgacca gaaccaggga aagtgcgagt
     6301 acgatagatt gcatcttttt cacaggttac tccttttatc attgcccgtt atgggtcgat
     6361 atccggagat tacgcgatga gaacgacggt tgttattctc cgcaatccta atctctaagt
     6421 taccgcattt attgagaatt tctccttttt ccctgtcgtt tttctccata atttcttcat
     6481 aaaaaagccg ggatgcataa aagcaaaccc ggcctgattg agataatgaa tagattttac
     6541 tgatgattca tcatcaattt acgcaacgca gcaaaatcgg cgggcagatt atgtgaaagc
     6601 aagggtaaat cagcacgttc tgccagctct tttggcagat ccaacgtttc accgagaatc
     6661 gcttccacgc tctctttaaa tttcgccgga tgcgcggtgc cgaggaacaa gccatattcg
     6721 cctggattca actggtcacg cagcgcacga tacgctacgg cagcgtgcgg ctccgaggtg
     6781 tagcccagtt cttttaactc acgcattgtc tgttgcgtgg tttcatcatc cacggctgca
     6841 taacccagct ctttcagttg ccagattttg cggcggaaca actcttccac acgcggccag
     6901 ttgttcggct gactcacgtc catcgcgttg gataacgtcg cctgagtcgc tttgggtgac
     6961 cactgaccgt cgtgcaggaa acgtggcacg gtatcgttca cgttggtcgc agcaataaaa
     7021 cgtttcaccg gcagaccgag tgacttcgcc agcagacccg ccgtcaaatc gccgaagttt
     7081 ccgcttggca ccgagacaac cagctggttg cgcgcttcct gcggcagctg cgcaactgct
     7141 tcaaagtagt agcaaatctg cgccagcaaa cggctgatgt taatcgagtt agctgagttt
     7201 aaccccagcg ccactttcag ctcttcatca tcaaacgcct gcttcaccag cgcctgacag
     7261 gcatcgaaat cgccgtcgat ggcaacagtt tcgatattgc cgcccaatgt acagaacagt
     7321 ttttcttgca gtggactgat tttgcctcgt ggatagagga taaccacttt cacattcggt
     7381 aaaccgtaga aagcatgagc cactgccgct ccggtatcac cggaggtcgc ggtcagaatg
     7441 gtcactggct tatcgcccgc aatatgggtc agcatttgtg ccataaagcg accgccgaaa
     7501 tctttaaatg ccagcgttgg cccgtggaac aattccagac aaccgacatc gctttcaaca
     7561 ttggcgaccg gagccgggaa ggcaaacgcc gcgcgcacgc gctcttccag gatttcctgc
     7621 gggatttcat caccaataaa cgccgagagg atcttcgcac tgcgggtgac aaaatccagc
     7681 ttcagcatct catcaatttc agtcaggctg aattccggca ggtcgtgcgg aaaaaacagc
     7741 ccctgatttt tgcccaaccc ctgggttacg gcttgcgcaa agctgacctg ctcattgtga
     7801 tctttcagat tgtagagttt catttagttt tccagtactc gtgcgcccgc cgtatccagc
     7861 cggcaaatat gaacaaaacc ttcctgattt tgcaggtagt tcttacccaa ccagtcggca
     7921 acgcgctggg cggtatccgg cttgtcacac agagcgaaca aggtcgggcc ggagccggag
     7981 ataccgctcg ctaccgcgcc gatttccgca accgcctgcc gcgcctgccg gaagccaggc
     8041 agtaaccgtt cacggtaggg ttcagcgata acatctttca tcagcttcgc ggcaagctca
     8101 ggctgacggg aatagcaggc gtgaatgaag ccagccagat gtcgcccgtg cgcaatgcaa
     8161 tcctggcggc gatactgcgc cggtaaaata gccctggctt ctgccgttga gactttaatc
     8221 cccggatacg ccagcaccca cagccactca tcaaaccctg gcacttgctg gctgatgatg
     8281 tcgttttctt cgatcatcaa ctgcatacca ccaagaaaac acggtgccac gttgtcgtaa
     8341 tgaatgctgc cggagattcg tccttccagc tcgcccatca aagccagcaa acgagtgtca
     8401 ttaagcggct tgccacagtg ttcattcatc gccatcagcg ccgcgaccac cgaacaggcg
     8461 ctggagccta agcccgaacc gattggcata ttcttttcca gagtcatcgc cactggaatt
     8521 tgcttgccaa gctcctggca aaaacgctcc cagcactgat aaacgatatt ttcccgtggt
     8581 tctgacggca gcttatcggc aaagcgtccg aggttgttga gactgaatgt ctctgccgcc
     8641 tcaaccgtga ctacatctcc gagcaatgca ccatcaacag gtgtcaccgc cgccccgagc
     8701 acatcaaacc cgacgctcat attggcactg gaagccgggg cataaacttt aaccatgtca
     8761 gactcctaac ttccatgaga gggtacgtag cagatcggca aagacaccgg cagctgtaac
     8821 gtcattgccc gcaccatatc cgcgcagcac caacggcagc ggctgataat agtggctata
     8881 aaaggccagg gcgttttcgc catttttcac tttgaacagc ggatcattac catccacttc
     8941 ggcaatcttc acgcggcagg caccatcttc atcaatatta ccaacatagc gcaaaacttt
     9001 tccttcatca cgggccttcg ccacgcgcgc ggcaaagaga tcgtcgagct gtgacagatt
     9061 cgccataaaa gcggcaacat caccctcagc gttaaactct gcgggcagca caggttcaat
     9121 ttcaatatcc gccagctcca gttcacgtcc cgtttcacga gcgagaatca atagcttacg
     9181 cgctacatcc ataccagaaa gatcatctcg cggatccggt tcggtataac ccatttcccg
     9241 cgccagagta gtcgcctcgg agaaactcat gccttcgtct aacttgccga agatataaga
     9301 aagcgaacct gaaagaatgc cggagaactt catcaattca tcaccagcat tgagcagatt
     9361 ttgcaggttc tcaataaccg gtaatccagc cccaacgttg gtgtcataga ggaatttacg
     9421 ccgcgatttt tccgccgcat gacgcaacag atggtagtaa tccatcgacg aggtgttggc
     9481 ctttttgttc ggcgtgacaa cgtggaaacc ttcgcgcaag aagtcggcat attgatccgc
     9541 cactgcctgg ctggaggtgc agtcaacaat gaccgggttc agcagatgat attctttcac
     9601 gaggcgaatt aagcgcccga gattaaacgg ctctttggct tgcgccagtt cttcctgcca
     9661 gttttccaga tttaggccat gcacattggt gagcagtgcc ttcgagttgg caacaccgca
     9721 gacacgtaag tcgatatgtt tattcttcag ccagctttgt tgacgcttca gttgctccag
     9781 cagcgcaccg ccaacgccac cgacgccaat cacaaacact tcgataacct gatcggtatt
     9841 gaacagcatc tgatgagtaa cgcgcacgcc agtggtcgca tcatcgttat ttaccacgac
     9901 agagattgag cgttcagaag atccctgagc aatggcgaca atgttgatat tggcgcgggc
     9961 cagcgcggca aagaatttcg ccgagatccc acgcaaggtg cgcataccat cacctaccac
    10021 cgagataatg gccagccgtt ccgtcaccgc cagcggctcc agtaagcctt ctttcagttc
    10081 caggtagaac tcttcctgca ttgcccgttc agctcgcaca cagtcgcttt gtggaacgca
    10141 gaaactgata ctgtattcgg aagatgattg cgtaatcagc accacggaaa tacgggcgcg
    10201 tgacattgca gcaaagacgc gcgccgccat gccgaccatt cctttcatcc ccggaccaga
    10261 aacgctgaac attgccatat tattcagatt ggaaatgcct ttgaccggta attcgtcttc
    10321 atcacggctg gcaccaatga gcgtacctgg tgcttgagga tttccggtat ttttaatcag
    10381 gcaagggatc tggaactggg cgatgggggt aatggtgcgg gggtgaagaa ctttagcgcc
    10441 gaagtaggaa agctccatcg cttcctggta ggacatcgac ttcaacaacc tcgcatcggg
    10501 cacctgacgc gggtcgcagg tatagacccc gtcaacgtcc gtccaaatct cgcaacaatc
    10561 ggcgcgtaaa caggcagcca gcaccgccgc ggagtagtcg gaaccgttac gtccaagtac
    10621 caccagttcg cctttttcat taccggaggt gaaacctgcc atcaacacca tgtgatcagc
    10681 cggaatgcgg cttgccgcaa tacggcgggt ggactcagca atatcgacgg tagattcgag
    10741 gtaatgcccc actgccagca gtttttcgac cggatcgata acggtaacgt tgtgaccacg
    10801 cgcttctaac acgccggcca taatggcgat cgacattttc tcgccacggc aaatcagcgc
    10861 agcgttgatg ctatccgggc actgccccaa caaactaatg ccatgcagga catgttttat
    10921 ttgggcaaat tcctgatcga cgaaagtttt caattgcgcc agcgggaacc ccggctgggc
    10981 ggcggcgagt cccgtcaaaa gttcggcaaa aatacgttcg gcatcgctga tattgggtaa
    11041 agcatcctgg ccgctaatgg ttttttcaat catcgccacc aggtggttgg tgattttggc
    11101 gggggcagag aggacggtgg ccacctgccc ctgcctggca ttgctttcca gaatatcggc
    11161 aacacgcaga aaacgttctg catttgccac tgatgtaccg ccgaacttca acactcgcat
    11221 ggttgttacc tcgttacctt tggtcgaaaa aaaagcccgc actgtcaggt gcgggctttt
    11281 ttctgtgttt cctgtacgcg tcagcccgca ccgttacctg tggtaatggt gatggtggtg
    11341 gtaatggtgg tgctaatgcg tttcatggat gttgtgtact ctgtaatttt tatctgtctg
    11401 tgcgctatgc ctatattggt taaagtattt agtgacctaa gtcaataaaa ttttaattta
    11461 ctcacggcag gtaaccagtt cagaaactgc tatcagacac tcttttttta atccacacag
    11521 agacatattg cccgttgcag tcagaatgaa aagctgaaaa atacttacta aggcgttttt
    11581 tatttggtga tatttttttc aatatcatgc agcaaacggt gcaacattgc cgtgtctcgt
    11641 tgctctaaaa gccccaggcg ttgttgtaac cagtcgacca gttttatgtc atctgccact
    11701 gccagagtcg tcagcaatgc catgactcgt tcgcgtaaag cttgcagttg atgttggtct
    11761 gccgttgtgt cacttttcgt cggttgttgt attaatgttg ctaattgata gcaatagacc
    11821 atcaccgcct gccccagatt gagcgaagga taatccgcca ccatcggcac accagtaaga
    11881 acgtcagcca acgctaactc ttcgttagtc agcccggaat cttcgcgacc aaacaccagc
    11941 gcggcatggc tcatccatga agatttttcc tctaacagca gcaccagttc tactggcgtg
    12001 gcgtagtaat gatatttcgc ccgactgcgc gcagtggtgg cgacagtgaa atcgacatcg
    12061 tgtaacgatt caaccagtgt cgggaaaact ttaatattat caataatatc accagatcca
    12121 tgtgcgaccc agcgagtggc tggctccaga tgtgcctgac tatcgacaat ccgcagttcg
    12181 ctaaaaccca tcgttttcat tgcccgcgcc gctgccccaa tattttctgc tctggcgggt
    12241 gcgaccagaa taatcgttat acgcatattg ccactcttct tgaccaaatg accgcgaaca
    12301 gggtgatcac tgtcaactta ttacgcggtg cgaatttaca aatccttaac gtaagtcgca
    12361 gaaaaagccc tttacttatc ttaaaaaagg ctaaactatt tcctgactgt actaacggtt
    12421 gaattgttaa aaaatgctac atatccttct gtttacttag gataatttta taaaaaataa
    12481 atctcgacaa ttggattcac cacgtttatt agttgtatga tgcaactagt tggattatta
    12541 aaataatgtg acgaaagcta gcatttagat acgatgattt catcaaactg ttaacgtgct
    12601 acaattgaac ttgatatatg tcaacgaagc gtagttttat tgggtgtccg gcccctctta
    12661 gcctgttatg ttgctgttaa aatggttagg atgacagccg tttttgacac tgtcgggtcc
    12721 tgagggaaag tacccacgac caagctaatg atgttgttga cgttgatgga aagtgcatca
    12781 agaacgcaat tacgtacttt agtcatgtta cgccgatcat gttaatttgc agcatgcatc
    12841 aggcaggtca gggacttttg tacttcctgt ttcgatttag ttggcaattt aggtagcaaa
    12901 catgcagacc ccgcacattc ttatcgttga agacgagttg gtaacacgca acacgttgaa
    12961 aagtattttc gaagcggaag gctatgatgt tttcgaagcg acagatggcg cggaaatgca
    13021 tcagatcctc tctgaatatg acatcaacct ggtgatcatg gatatcaatc tgccgggtaa
    13081 gaacggtctt ctgttagcgc gtgaactgcg cgagcaggcg aatgttgcgt tgatgttcct
    13141 gactggccgt gacaacgaag tcgataaaat tctcggcctc gaaatcggtg cagatgacta
    13201 catcaccaaa ccgttcaacc cgcgtgaact gacgattcgt gcacgcaacc tgctgtcccg
    13261 taccatgaat ctgggtactg tcagcgaaga acgtcgtagc gttgaaagct acaagttcaa
    13321 tggttgggaa ctggacatca acagccgttc gttgatcggc cctgatggcg agcagtacaa
    13381 gctgccgcgc agcgagttcc gcgccatgct tcacttctgt gaaaacccag gcaaaattca
    13441 gtctcgtgct gaactgctga agaaaatgac cggccgtgag ctgaaaccac acgaccgtac
    13501 tgtagacgtg acgatccgcc gtattcgtaa acatttcgaa tctacgccgg atacgccgga
    13561 aatcatcgcc accatccacg gtgaaggtta tcgcttctgt ggtgatctgg aagattaatc
    13621 ggctttacca ccgtcaaaaa aaacggcgct ttttagcgcc gtttttattt ttcaacctta
    13681 tttccagata cgtaattgat cgtccattgt aacttcttta ctggctttca ttttcggcag
    13741 tgaaaacgca taccagtcga tattacgagt cacaaacatc atgccggcca gcgccaccac
    13801 cagcacactg gttcccaaca acagcgcgag ggtaaacaac atgctgttgc gccagccttt
    13861 caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg cgattatcca
    13921 tgccacggta aaaccgatat gttcagaaag cgccagcaag agcaaataaa acatcaccaa
    13981 tgaaagcccc accagcaaat attgcattgg gtgtaaacgt tgcgcggtga gcgtttcaaa
    14041 aacaaagaac gccataaaag tcagtgcaat cagcagaatg gcgtacttag tcgcccggtc
    14101 cgttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg ggaagttttc
    14161 ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa accagctgct
    14221 ttgccattgc gcctgaaaac ctgactcgct aacttcccgt ttggctggta gaaaatcacc
    14281 taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccggcagg
    14341 caccacagaa agatcgccgg taccgcttaa attcagtgcc atattcagct gcaggttctg
    14401 cttccgccag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt gctctaaccc
    14461 ggtaccgggt tcaatggtca gtgccgttcc gttaacttca ggcgctttca ccacaccaat
    14521 accacgcgca tccccgacgc taatcacaat aaagggcttg cctaagatga tatttggcgc
    14581 gttgagctcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca
    14641 gacctgaccg gtataaatcc ctatcttgcg ttcttccacg ttttgattgc catcaaccat
    14701 caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg ttttatcctc
    14761 ttcctgcacc gtataaagct cggtcacagg aatagcgata agcggcccaa cgagtttttg
    14821 cggcccgctg gtactttggc gaatcgcgtc ttccacatcg ctacggtaat cggcacgttc
    14881 gacaatcact tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacaa
    14941 ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg caatggcagc
    15001 aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgaag
    15061 cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg
    15121 ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat ttgcacgcgg
    15181 caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac cactaccggt
    15241 atccagcacc ttaagggcga cgtgttcctg atccacttcg gcgcttagcg ttatgcgacc
    15301 gctctcgggg gtaaaatcga tggcgttatc cagtaaattc cccagcgcct gctccagtaa
    15361 cgccggttca gcaacaacat taacctcagt gggcataaca tgtagagtga tgtttttttc
    15421 tgccaactgc acggtgcgcg cttcgctgac gcggcgaaat aatgccgcca catcaacaac
    15481 agtcagaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg tttctaccaa
    15541 tgcctgcata cgcgcatttt gcgtcagaat gttgtcggta aaacgagcca ccacttccgg
    15601 tggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg ggctttttag
    15661 ctcatgggtt aacgcataaa catactgctc aatatagttt ttcccttcca gcttcacgcg
    15721 catactttcc agcgcctgcg caagcttacg caactcgcta ctgccgagtt caggaagagg
    15781 aacgggctta ttgtcagtga cggaatcggc atagcgagtg agccgggcaa tggagcggtt
    15841 gatccaccaa accatgcctg cgccaatcac cagtgcaatc cccagcaaaa tggcgctggc
    15901 ccataaaatt cgccgctcgc tacgcttaat gaccggagcc atcgccgcgt tcggtttgcc
    15961 tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa catacatcac
    16021 cgaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact gaccatgcaa
    16081 cgttagccag acgtcattcc agcgcgaata atcctgccca acggctttat ttgccgaatc
    16141 gtacaatact ttaccctgcg aatcggtcat atagacatga tattcattgc gcactttgtt
    16201 aatgccaccg atattggcgc gaaacggacg atgttgtagc tgattaaacg cctgcgccag
    16261 ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg ccagcaacgt
    16321 tgcggtgtcg atcaacgtcc cctccgttgc tcttcgcacg cccggtttaa cttctttgac
    16381 aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc ccagcagcaa
    16441 ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg atgagtatta
    16501 atcggtgaaa gatcggggtt gatggcacgc agcttggcat gcaacgtttt aatgtgggta
    16561 tcaacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat cagttgctgg
    16621 cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc
    16681 gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt caattcaaaa
    16741 tgaccaatac ggatgacggg agacggcgac gagaacttct tcacccgacg cagtaaggtg
    16801 cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc gtcagcgcca
    16861 atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa cagtacaggt
    16921 aacgccggat ggagcgccag taactggctg cacaattcaa agccgctgat atcaggcaaa
    16981 ccaacgtcga gaatcatgac gtcgggaacc agctggcgag ctttatccag caccggcaag
    17041 cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac cagcgtgtcg
    17101 gctatccctt gctcatcttc cactaaccag accgtttccc gttgtataaa tcgcctctgt
    17161 tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca
    17221 actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tggcgtcata aaagcgcacg
    17281 acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc gccctgagct
    17341 ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca agaaatagcc
    17401 gcatcggagg tatcttccgc cagacccaat ccccctttaa taccacctgt tttcgcccgg
    17461 ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac aacaattttg
    17521 tgatccgggc cgaccatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc
    17581 aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt catattgtta
    17641 ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga
    17701 acaaaatctc aatactttta tttccgtcaa gccctaaaac atactctact aagaaaaaaa
    17761 cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc aggtgctcgt
    17821 tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct
    17881 ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt
    17941 attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt
    18001 atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga
    18061 ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgcattca
    18121 agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg
    18181 gtattcgccc gccgctacgt ctgggtgaat tcacaatgcc agagcacaaa tttgtcaccc
    18241 tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct
    18301 ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcggc aacgcgccga
    18361 ccattccgcc agtgctctac ggcctgaatg aaacgcgtcc gagtcaggat aaagacgacg
    18421 aacaagaggt attctatacc accgcgttag cccaggatca ggcagatggc tatgtactga
    18481 cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat gaaggtctgg
    18541 gaaccggcgt gcaggagttt atcctgacgg tatacggaac gtgcatgcca atgctcaacc
    18601 tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagac gccaaagcgg
    18661 gagatcgccc aattaatctg cgctgtgaac tgctgattcc tatccgccgt taacgctgca
    18721 gctcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca acgacccagc
    18781 cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag ttacgcagac
    18841 gtaagcggcg cgctgcccat gctggtaatc cgagaatcgt actcaccaga catcccagtg
    18901 caataccatg gctcaccagc agcggtcggc ttccctgcgg taagtcccgg caagattcca
    18961 gtgccgcatt gacgcgaccg ctgagctcct gcattgactc gccttcagga atacgcccgt
    19021 caacggtgcc attgaccagc tgccgacgcc agttctcttc ttcttcggtc agagaatcga
    19081 tatgtctttt ttccagcaca cccatgttta attcacgcag gcgagaatca aagatgatgt
    19141 cacagccgca ggcctgggcg atgatttccg ccgtacgctg ggtgcgtcct aaatcgctac
    19201 tgataatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc gcctgttgct
    19261 cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg gcgttccact
    19321 gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt atactgctgt
    19381 aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga ccaccaatcc
    19441 cgctaaaatt caggccattc tgcaggcatt tcacgagatc ttcggcgaag gatcctgcca
    19501 tattgcatcc gttgccgtcg agagcggtgt accggaacag ccctttggca gtgaggaaac
    19561 gcgcgctggc gcacgaaatc gggtagcaaa tgcccgccgt ttacttccag aggctgattt
    19621 ttgggtggcg attgaagctg gcatcgatgg tgacagcact ttcagttggg tggtgattga
    19681 aaacaccagc cagcgcggcg aggcgcgttc ggctactttg ccgttacctg cggtgattct
    19741 gcagaaagtg cgtgaaggtg aggcgcttgg tcccgtgatg tcgcgttata ccggtattga
    19801 tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac tcactcgcgc
    19861 cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcacaatg ccgtgtatca
    19921 ggcattgtag gacggataag gcgttcacgc cgcatccggc attcggtgca cgatgcctga
    19981 tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc gcttttcagc aacacctctt
    20041 ccagccactg gcgcagctcc acgggcgcgg ctttcaggct gttagatcca cgcgtaatcg
    20101 tcgcgatgcc cgcgccgagt tcatttttta actcacgctg actcatttcg ccacgcaaca
    20161 gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc gtcagcatca
    20221 ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac
    20281 gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat tgttgggcca
    20341 taatatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt actcgctaaa
    20401 gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt ccgtggcgct
    20461 catcaacgtc ggtttatccc ccatgaaata acggtagtaa gcgtcataag ccagcacgtt
    20521 cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca cagcgtcgat
    20581 acgcccggcg ctgttgccaa gccaggttcg cacccgtcca ggtccggcgt tataagctgc
    20641 tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt aactggtgcc
    20701 aatgttgata ttcgtttccg gatccagcaa ttgcccaggg ctgctataac caggaataga
    20761 gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc cgctggcccc
    20821 taccggtgat ttcactttcg gattccaggc gctctcctga cgggcaatcg ccatcgcata
    20881 gctttgcggg atctctttcc cactggtgta acgtttgaaa agatcgttgt aagccagcgg
    20941 gaatcgctct tccagatgat cccaaagctt cccggcgctc gttgcctgaa cgctaagatc
    21001 ccaccattgg ttattgaaag cataccgagc cagttgagcc tgctctgttt ttgacttgct
    21061 cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt acatcaactc
    21121 gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct gcggcgcttt
    21181 atcaatcttc agctcatact cttctccgat gcgttgtgct gcaaccatcg ggtagaaacc
    21241 acgctgttgc atgagttgat gcaaaatctc tttcgcttca gcttcacgtc cgcgttccag
    21301 caataaatcc gcctgccagt aacgccattc atctttctct tttgcttcca tcggcagacg
    21361 cgccagccag gtattcaggc cgcgacgatc gccggtgcca agtgccattc gcacacggcg
    21421 ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca tcgcgccatt tcgcctgctc
    21481 gtcggtgaca tcgttgccca tcaaacgcca ggcgacgata tcgcgcagct cctgaatctg
    21541 atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg cattttcagc
    21601 atcctgccgc gccacactgg caaacgccac cgctgccatt tgacgggtaa aatcggtcgc
    21661 tccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg aaatgattgc
    21721 cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta ccaggccagt
    21781 gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat cttgtttacc
    21841 tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct tgccggttag
    21901 ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc atttcgcata
    21961 gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg ctaacaagcc
    22021 acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg tgcgagcggg
    22081 aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt gattcatcag
    22141 atcatcggtg atctggcggt attccaggta gggataaagc ggataatcct tcagtccagg
    22201 catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga tttgcgcgta
    22261 acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca
    22321 gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa gcgcatcctc
    22381 taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct aaccagtcat
    22441 tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg atcgaccaac
    22501 tgggattagg cggggaaaaa ggctacactt cgcctttgaa aactcatcat cacgataaac
    22561 atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt cggcaaagtt
    22621 gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc tggggcaaaa
    22681 attggtgtcc tgggtctgaa cggcgcgggt aagtctaccc tgctgcgcat tatggcgggc
    22741 attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat tggttacctg
    22801 ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg agtccattga agaagcggtt
    22861 tctgaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta cgccgatccg
    22921 gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat cattcaggct
    22981 cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg cggatgcgct acgtctgccg
    23041 gactgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt agcgttgtgc
    23101 cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa ccacctggat
    23161 gccgaatccg tggcctggct ggaacgcttc ctgcacgact tcgagggcac cgtggtggcg
    23221 attacccacg accgttactt cctcgataac gttgcaggct ggatcctcga acttgaccgc
    23281 ggtgaaggta ttccgtggga aggcaactac tcctcctggc tggagcagaa agatcagcgc
    23341 ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa agagctggag
    23401 tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct ggcacgcttt
    23461 gaagagctga acagcaccga atatcagaaa cgtaacgaaa ccaacgaact gtttattcca
    23521 cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa atcctacggt
    23581 gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcaat cgtcgggatc
    23641 atcggtccga acggcgcggg taaatcgacc ctgttccgta tgatctctgg tcaggaacag
    23701 ccggacagcg gcaccatcac tttaggtgaa acggtgaaac tggcatcggt tgatcagttc
    23761 cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg gctggatatt
    23821 atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt taactttaaa
    23881 ggggttgatc agggtaaacg cgttggtgaa ctttccggtg gtgagcgcgg tcgtctgcat
    23941 ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc aaccaacgac
    24001 ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc gggctgtgcg
    24061 atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacacacat cctggactac
    24121 caggatgaag gtaaagttga gttcttcgaa ggtaacttta ctgagtacga agagtacaag
    24181 aaacgcacgc tgggcgcaga cgcactggag ccgaagcgta tcaagtacaa gcgtattgcg
    24241 aagtaatgcg taaaatgccg gatgcggcgt gaacgcctta tccggcctac aaaccaagaa
    24301 atttcaatac gttgtgaatc tccaggcctg ataagacgcg gtaagcgtcg catcaggcat
    24361 tgagcaccga atgccggata cggcgtgaac gccttttccg gcctacaata gccttagtat
    24421 ccgcaatttc gtagcgggtc acccgtgctg attcttttaa accttgttta atctcgacag
    24481 cctcttccag agaggttttc aactcatcaa aaaacttatt ttatacctcg cctttgagcc
    24541 gtttcatcgc ggttatctct gctcccccat catctcccgc accagctcca cgcagcgcag
    24601 gaaacggctg tcataatcgt cctcttcaac ccgcacgaat tcgatattgt tctcttccag
    24661 catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa
    24721 accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc
    24781 atcaatcaac gcctgtacga acggatgctc acgcccttcg tactttttgc agaacgcctg
    24841 ggtagtgaca aaatcagtgt caataaacgc cactttattg gcatatttca ctgcaaaatc
    24901 aatgtattgt gcgtggccca gcgcgatttt atcgtaatcg gaatactgca atgcgatctc
    24961 atcaccgccg aggtgtgaaa agacataatc gcgaccatat tcccacgcac tggtggtatt
    25021 gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc caccaaggat
    25081 cgccacggta cgtacaaaga acggcttcac ttccgtagga atatattccc agtagcggaa
    25141 cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg gatcgaccag
    25201 caccgtatcg atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta
    25261 gatcaggtcc ggctgaatcc ccttttcagc cataaacttt ttgatgccgt tgctccacac
    25321 atcccagccg tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa tgcgaatatt
    25381 tttctgatat ttaaaagttt gtaataacca gcgcagacga tccggcaccg tgggctgctg
    25441 cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac ccataatgat
    25501 atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag
    25561 cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgtcggggaa attcaagccc
    25621 caaaaaacgg tgcaacgcct ccagcttttg cgcgctgggg cttttaattt tggcattcag
    25681 taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca
    25741 gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatc
    25801 tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc
    25861 tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa
    25921 ccgtgtttcg ccgcttctga aattcgttcc tgaccgctgg gcaccgggcg gatctcccct
    25981 gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc acgcaggctg
    26041 gaaaccatcg ccagcagtaa cgctaaatcg gcactggttt cggttacctt cacgccgccg
    26101 accacgttca caaatacatc ctgatcggcc atttgcagac caccgtgacg gtgcaagaca
    26161 gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg cggattcgcc
    26221 atcatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt tccttcccac
    26281 accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa aattgccgaa
    26341 gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag ctcattcacc
    26401 gcgccgaagc ggtttttatg gctgcgcaag gtgcgaaaac gggagtcggc atcaccatcc
    26461 agaagcaccg aacagtcgat acagtgttcc agcactttag ggccagccag cgagccatct
    26521 ttggttacgt gaccaaccat gacaatcgcc acaccgcgcg ttttggcgaa gcgcgtcaga
    26581 taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac atccgccata
    26641 tgcatcacct ggatcgagtc aattaccatc agtttcggtt gctcttcttc ggcaatcagg
    26701 cagatctgtt cgatgctggt ttccgacaac atattgagat tgtcagtcgg caggccaagg
    26761 cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cagtgacata cagcgttttc
    26821 atctgctggg ccagtttgca cagcgtttgc agtagcagcg tggatttccc cgcacctggg
    26881 ttaccgccaa tcagaatggc gcttcccggc accacgccgc cgcctagtac gcggtcgaac
    26941 tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga gagtttctgg
    27001 acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg cgccaccgtt
    27061 ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc
    27121 ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct
    27181 tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatggcgggc gagcaattac
    27241 ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc gtgacggatg
    27301 gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgct
    27361 gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg cgccagcggg
    27421 atttcatact cctgcgcgag gcgagtcaaa gttttcgctt tgtactgcgc gtctacgatg
    27481 tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg
    27541 gcggtcaggc gcagcttgtc gcgcagatat tcagcaaaga aagtaaagcc gccggaggca
    27601 atcgccactt tccagcccag cgtttccagc ttgagcacca gttgcgttaa gcctggcatc
    27661 agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc
    27721 acacggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttact
    27781 tccgccacca tctcgcccgt tccggccagt ttggcaattt catcaataca ttcaatctgg
    27841 atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg cgggattttc
    27901 cccagcgggg cgacatccag ttgcgcttca tgggccaggc gcgtagcccg tgcggtgagt
    27961 gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccatc
    28021 gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc acgaccatac
    28081 agcaaccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat
    28141 gaaagaggca gaccaggcca taaagagaca tcttcaggca ggtcgcacca ggtaatgtta
    28201 ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg
    28261 tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca
    28321 caaaactgaa attccggctg catcgggcag tgattgtcct gttctgtctt gccttgttag
    28381 tggcgctgat gcagggaacg tcatggttta gtcaaaacca ccagcgacaa cgtaatccac
    28441 agctggaaga actgacccgc cccctggcgc gtcaggtaac gctgaacgtt gcaccgctga
    28501 tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg
    28561 aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc gcacgttctg
    28621 gcgaaagcgt cgaagtgcgc gaccggctgg cgctcgacgg taaaaaagcg ggcggctatt
    28681 ttaaccagca gattgtcgaa ccaattgccg ggaaaaacgg accgctcggc tatctgcgcc
    28741 tgacgctcga cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca
    28801 ttttacgcct gatgttgctg ctttcactgg caatcggcgt agtgctgacc cgcacgctgc
    28861 tacagggtaa acgtaccctc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg
    28921 tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg
    28981 ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt aacctggcgg
    29041 tggaggagtg tatttttcgc cagatgcccg ccacgcagcg cgttctgttt ctctggcgca
    29101 atgccgacac ggtagtgatt ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc
    29161 ggatggaaga agataacgtc cgcctggcac ggcgcagtag cggtggcggc gcggtgttcc
    29221 acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac gataaaacca
    29281 tctccacgtc gattgtgctc aatgcgctga acgcgcttgg cgtcagcgcc gaagcgtccg
    29341 ggcgtaacga tctggtggtg aaaaccgccg aaggcgaccg caaagtctca ggatcggcct
    29401 atcgcgaaac caaagatcgt ggcttccacc acggcacctt gctgctcaat gccgacctta
    29461 gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattacct
    29521 cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggatcacc catgagcagg
    29581 tttgcgaggc cataaccaag gcctttttcg cccattatgg cgagcgtgta gaagcggaaa
    29641 tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaatcttt gcccgtcaga
    29701 gtagctggga atggaacttc ggtcaggctc cggcattctc gcatctgctg gatgaacgct
    29761 ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc acccgcgccc
    29821 aggtgtttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg cgactgcaag
    29881 gctgcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg gttgacttcc
    29941 cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg gtaaggtaac
    30001 tcgctgatta aaataccaaa ggaagggaat atgatcgatc tggaaaacca ggagcgggag
    30061 attatcaatt taatgttaag ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat
    30121 aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc tctaaaatag
    30181 atcgaaaaga cggaacggtt tagctcaaaa atcaaaagca aaatggcaga aatttatggt
    30241 tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa atgaatagat
    30301 tcgtgaatag atacttgaat agattttatt ttctatccat atgaaaaata atagtaaatc
    30361 tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtct cccactccgg
    30421 cttcacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc agcagaacgg
    30481 attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg gcagtggtct
    30541 gctcgtgagt gcggatgtgg tcagacacgg tacagatggt cagggctttc gcgccaaatt
    30601 ctgcagcgac gccgtagata ccagccgctt ccatttccac gccgaggatg ccgtattttt
    30661 ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag aacaggttac
    30721 caacgcgagc atcaatgccc agtgctttag ccgcgtctac cgcgttacgc accatgtcaa
    30781 agtcagcgat agcggcaaag tcatgatctt taaaacggat gcggttaact ttggaatcgg
    30841 tgcaggcacc cataccgata acgacgtcgc gcagttttac gtgcggcaga actgcgccac
    30901 aggaacccac gcggataatt ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga
    30961 tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg cctttgtaag
    31021 taccggtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag
    31081 tttcagcaat atacttcgca cgcagcgggt cgcctggcat caaaactacg tcagcgaaat
    31141 cgcccatttc tgcattaatg tgtggggtag ccattgtttt atccttatca acatgttttc
    31201 accctctccc tgtgggagag ggccggggtg agggcatcaa gccgcaccca ttatcagaac
    31261 atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt ctggccgata
    31321 tccgcgaagg tttcacggtg acccagtgaa cccggtttta ctttcgggcc gtaaaccagt
    31381 accggaatgt gttcacgcgt gtggtcagta ccggtccagg tcggatcaca accgtggtca
    31441 gcagtgagga tcaggatgtc gtcatcgcgc agcaaagaca tcagctccgg cagacggcgg
    31501 tcgaacagct ccagacccgc agcataaccg gcgacgtcgc gacggtggcc ccaggaagag
    31561 tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt catctctttg
    31621 atggtggcgt caaacagcgc gtccaggcca gttgctttca ctttcttggt gataccgcag
    31681 ttggcgtaga tgtctgcaat tttaccgaca gaaaccacct gaccgtgttt ttcatcaacc
    31741 agtttctgca gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg gttaccggta
    31801 cgctggaagt taccggcttt gtcaccgata aacggacgag cgataacacg accgatgttg
    31861 tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gttcgtagag tttatccagg
    31921 ccaaaggttt cttcgtggca ggcaatctgg aacacggagt cagcggaggt atagaaaatc
    31981 ggcttgccgg ttttcatgtg ctcttcgccc agctgatcca gaatgaccgt accggaagag
    32041 tggcagttac cgaggtaacc cggcagatta gcgcgttcga ccagtttatc cagcagctct
    32101 tgcgggaagc tgttttcgtg atcggagaag tatccccact caaacagaac cgggacaccg
    32161 gcgatttccc agtgaccaga cggggtatct ttaccggatg acatttcgtg cgcccatgcg
    32221 tacgcgccga taacttcagc gttgccgtcc attcccgccg gaatgaaacc ggtagaacct
    32281 tcgtgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg gcctttacga
    32341 ccgttatcag cttcgccttt ggcacaagct tctgcgatat gacccagggt gtcagccccg
    32401 acgtcaccaa agcgttcagc atcttctgta gcgccgatgc cgaatgagtc cagcaccata
    32461 ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcagatca
    32521 gtataccgtt attcgctgat acggcgatag acagttggtg tgctttccgg tgctttatcg
    32581 gcaagtttaa ttgccgcttt caccgctttc gccgcttcct gccagctgtt ttcgtctttc
    32641 gcgtggataa ccgccagcgg acgctgaccg tctacttggt cgcccagacg cgccatatca
    32701 gtaaagccga cgctgtaatc gatggtgtca gatgcctgac gacgaccgcc gcccattgca
    32761 accactgcca tccccagcgc gcgggtatcc atttcactga caaaaccttc ggtatcagca
    32821 tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg
    32881 gtcgggcctt tttgtgccgc taccatacga ccaaagactt ctgccgcttt accgttgtcc
    32941 agcaccgcct gcaatttcgc gcgcgcttca gcgtcatctt tcgccagttt gccggagatc
    33001 agcatctcca cgcacagcgc catcgtgaca tcaaacagac gcgggttacg atattcaccc
    33061 gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc
    33121 tgattcatgt cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca
    33181 atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact
    33241 ttcacgtcca tcaccagcgc atccagacct tccgccagtt tcttcgccag gatcgaggca
    33301 gtgatcagcg ggatggagtc cacggttgcg gtaatatcac gggtcgcgta gaaacgttta
    33361 tccgccggag ccagtgagct ggtctgaccg ataatcgcca cgccgacgtc tttaataatt
    33421 tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg
    33481 agcgtaccgc cagtatgacc gaggccgcga ccggagatca tcggaatata gccgccgcag
    33541 gctgcgacca tcggccccaa catcagcgaa gtgacatcgc cgacgccgcc ggtggaatgt
    33601 ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt tcctgaatct
    33661 cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc
    33721 atcgcgaggg cggcaatctg cccttcggag atagtgttgt cgcgaatgcc gttgataaag
    33781 aaacgaattt cttcatcgtt cagcgcatga ccatcacgtt ttttacgaat aatttcttgt
    33841 gcgagaaaca aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc
    33901 tacgccttat ccggcctacg ggtagatacg tctcgtaggc ctgataagcg cagcgcatca
    33961 ggcgtaaagc atcttgctta gtagctgctg gcgctcttac cgtcaccgtg gcccagcgct
    34021 ttcaacaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atctgcccag
    34081 tcagcaccga acagttcatc tgcgatggcg agatatttct gcgcatcttc cgcagtacgc
    34141 acgccgcccg ccggtttaaa accaacggtt ttttctacgc ccatatcacg gatcacttcc
    34201 atcatgatgc gcgcgctttc cggcgtcgcg ttcactgcca ctttaccggt agaggttttg
    34261 atgaagtcag cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct
    34321 ttcagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct
    34381 ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac
    34441 gggaacacaa cgtcaacttc atcagcaccg taggcgattg ccgcacgggt ttctgccagc
    34501 gcgatttcga tgtcatcgtt accgtgtggg aagttggtta ccgtagcgat tcggatttcc
    34561 ggggtgccct gctctttcag cgttttgcga gcaatcggga taaagcgagg atagatacag
    34621 atagcggcgg tattgccgac cggagttttg gtctgatgac acagagcaat tactttctcg
    34681 tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt
    34741 gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt
    34801 ttgttagtat tctaacatct actccgcaac acacttcgat atacatcaca attaaggaaa
    34861 tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg
    34921 aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa
    34981 tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact
    35041 gcaacaacgt tgccagcaaa ttgtcaccag cccagtgctt agcccggagc agaagcgcca
    35101 ttttctggct ctggaagcag aaaacaatct gccttatccg cagcttcctg ccgaagcccg
    35161 ccgcgcgctg gatgagggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc
    35221 gcgctatgtc ttacccgatt acgcccgttt tctggcgaac g
//
LOCUS       DAJTNA010000018        34955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTNA010000018 DAJTNA010000000
VERSION     DAJTNA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34955
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.018"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(236..1495)
                     /locus_tag="OO161_000777"
     CDS             complement(236..1495)
                     /locus_tag="OO161_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5461944.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEVTFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALSKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(1684..2547)
                     /locus_tag="OO161_000778"
     CDS             complement(1684..2547)
                     /locus_tag="OO161_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5461945.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            2674..3390
                     /gene="rph"
                     /locus_tag="OO161_000779"
     CDS             2674..3390
                     /gene="rph"
                     /locus_tag="OO161_000779"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5461946.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            3456..4097
                     /gene="pyrE"
                     /locus_tag="OO161_000780"
     CDS             3456..4097
                     /gene="pyrE"
                     /locus_tag="OO161_000780"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5461947.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(4134..4730)
                     /gene="slmA"
                     /locus_tag="OO161_000781"
     CDS             complement(4134..4730)
                     /gene="slmA"
                     /locus_tag="OO161_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5461948.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(4837..5295)
                     /gene="dut"
                     /locus_tag="OO161_000782"
     CDS             complement(4837..5295)
                     /gene="dut"
                     /locus_tag="OO161_000782"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5461949.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(5273..6493)
                     /gene="coaBC"
                     /locus_tag="OO161_000783"
     CDS             complement(5273..6493)
                     /gene="coaBC"
                     /locus_tag="OO161_000783"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5461950.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            6665..7333
                     /gene="radC"
                     /locus_tag="OO161_000784"
     CDS             6665..7333
                     /gene="radC"
                     /locus_tag="OO161_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5461951.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            7550..7786
                     /gene="rpmB"
                     /locus_tag="OO161_000785"
     CDS             7550..7786
                     /gene="rpmB"
                     /locus_tag="OO161_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5461952.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            7807..7974
                     /gene="rpmG"
                     /locus_tag="OO161_000786"
     CDS             7807..7974
                     /gene="rpmG"
                     /locus_tag="OO161_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5461953.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            8072..8881
                     /gene="mutM"
                     /locus_tag="OO161_000787"
     CDS             8072..8881
                     /gene="mutM"
                     /locus_tag="OO161_000787"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5461954.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(8920..9399)
                     /gene="coaD"
                     /locus_tag="OO161_000788"
     CDS             complement(8920..9399)
                     /gene="coaD"
                     /locus_tag="OO161_000788"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5461955.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(9407..10684)
                     /gene="waaA"
                     /locus_tag="OO161_000789"
     CDS             complement(9407..10684)
                     /gene="waaA"
                     /locus_tag="OO161_000789"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5461956.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     LLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            11097..12155
                     /gene="rfaQ"
                     /locus_tag="OO161_000790"
     CDS             11097..12155
                     /gene="rfaQ"
                     /locus_tag="OO161_000790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5461957.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            12152..13276
                     /locus_tag="OO161_000791"
     CDS             12152..13276
                     /locus_tag="OO161_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5461958.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            13269..14066
                     /gene="rfaP"
                     /locus_tag="OO161_000792"
     CDS             13269..14066
                     /gene="rfaP"
                     /locus_tag="OO161_000792"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5461959.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            14082..15098
                     /gene="waaO"
                     /locus_tag="OO161_000793"
                     /gene_synonym="rfaI"
     CDS             14082..15098
                     /gene="waaO"
                     /locus_tag="OO161_000793"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5461960.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            15115..16110
                     /locus_tag="OO161_000794"
     CDS             15115..16110
                     /locus_tag="OO161_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR5461961.1"
                     /translation="MNEFIKERFSYLADNKKENVPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            16120..16812
                     /gene="rfaY"
                     /locus_tag="OO161_000795"
     CDS             16120..16812
                     /gene="rfaY"
                     /locus_tag="OO161_000795"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5461962.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            16838..17863
                     /locus_tag="OO161_000796"
     CDS             16838..17863
                     /locus_tag="OO161_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR5461963.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQDSCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            17948..18931
                     /locus_tag="OO161_000797"
     CDS             17948..18931
                     /locus_tag="OO161_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5461964.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNTC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRDCNLPGKRDKRRAKILASKNYILWRLVRLVNRKKNKR"
     gene            18977..20230
                     /locus_tag="OO161_000798"
     CDS             18977..20230
                     /locus_tag="OO161_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5461965.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(20300..21280)
                     /gene="rfaC"
                     /locus_tag="OO161_000799"
     CDS             complement(20300..21280)
                     /gene="rfaC"
                     /locus_tag="OO161_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5461966.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAENAAMI"
     gene            complement(21284..22330)
                     /gene="rfaF"
                     /locus_tag="OO161_000800"
     CDS             complement(21284..22330)
                     /gene="rfaF"
                     /locus_tag="OO161_000800"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5461967.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(22340..23272)
                     /gene="rfaD"
                     /locus_tag="OO161_000801"
     CDS             complement(22340..23272)
                     /gene="rfaD"
                     /locus_tag="OO161_000801"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5461968.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            23486..24682
                     /gene="kbl"
                     /locus_tag="OO161_000802"
     CDS             23486..24682
                     /gene="kbl"
                     /locus_tag="OO161_000802"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5461969.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            24692..25717
                     /gene="tdh"
                     /locus_tag="OO161_000803"
     CDS             24692..25717
                     /gene="tdh"
                     /locus_tag="OO161_000803"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5461970.1"
                     /translation="MKALSKLKAEEGIWMADVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            25956..26990
                     /gene="waaH"
                     /locus_tag="OO161_000804"
     CDS             25956..26990
                     /gene="waaH"
                     /locus_tag="OO161_000804"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR5461971.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(26977..27936)
                     /locus_tag="OO161_000805"
     CDS             complement(26977..27936)
                     /locus_tag="OO161_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5461972.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAAGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(27940..29223)
                     /gene="envC"
                     /locus_tag="OO161_000806"
     CDS             complement(27940..29223)
                     /gene="envC"
                     /locus_tag="OO161_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5461973.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADV
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(29233..30777)
                     /gene="gpmM"
                     /locus_tag="OO161_000807"
                     /gene_synonym="pgmI"
     CDS             complement(29233..30777)
                     /gene="gpmM"
                     /locus_tag="OO161_000807"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5461974.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31022..31453
                     /locus_tag="OO161_000808"
     CDS             31022..31453
                     /locus_tag="OO161_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5461975.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            31595..31846
                     /gene="grxC"
                     /locus_tag="OO161_000809"
     CDS             31595..31846
                     /gene="grxC"
                     /locus_tag="OO161_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5461976.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            31909..32376
                     /gene="secB"
                     /locus_tag="OO161_000810"
     CDS             31909..32376
                     /gene="secB"
                     /locus_tag="OO161_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5461977.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTATLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            32376..33395
                     /gene="gpsA"
                     /locus_tag="OO161_000811"
     CDS             32376..33395
                     /gene="gpsA"
                     /locus_tag="OO161_000811"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5461978.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            33475..34296
                     /gene="cysE"
                     /locus_tag="OO161_000812"
     CDS             33475..34296
                     /gene="cysE"
                     /locus_tag="OO161_000812"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5461979.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(34349..34822)
                     /gene="trmL"
                     /locus_tag="OO161_000813"
     CDS             complement(34349..34822)
                     /gene="trmL"
                     /locus_tag="OO161_000813"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5461980.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         8746 a         8289 c         8722 g         9198 t
ORIGIN      
        1 gtgccgtcgt agccgcaaag gtacgcgctc aaatatatag attctgccgc cgccataatc
       61 aaaagatcat caatatctat ttctaataat tcggctgctc tgttaagcct gaaaaaaggg
      121 gcgggtggta attttttcat tgcgtcacct tttacgctgc gccttatttt gtgattgagc
      181 caggcggtaa ggcttcccgc ttttcgcccc gtcgagctag gctcaatcta ttcagttagt
      241 tcgcccttct gagaggcgtt acgttgtagt cttttccgct ttccagtgcc accagcagat
      301 cagcccactg tgccagtgct gctttacgct catcaaaata ctgatggcgg ttataaatcc
      361 cctctacgcc cttaatgcga tgattgaggc atcgttccgc cacaacagga tcaacaccca
      421 acgctgctaa atgagtacgt gcggtgcgtc gaaagtcgtg aatcgtgaaa ttaggcacat
      481 ccggcatttc ggcgcgaacc ttagacagtg ctacgggtaa agtgctttcc tgaatatgtg
      541 ggatcattct gttttgcatc ttcctggccg gaagcaccca tgcgctatta catgaaaagg
      601 tgtgtagctc tctcaaccat tcaacggctg gggaaggtag aggtatatca atagggtctc
      661 cgtttttgct gcgttcttcc ggcaaatgcc ataccgcacc atctaaatca aactcttccc
      721 atcgtgcggc gcataattcc attttgcgga cgcataacgc tagcagtaat ttgaacgtga
      781 cttcgttctg gcgactgaat cccttagccg tgcgcatggc tttgaatagc tggattaact
      841 catcgcgggt taaccagcgg tcacgggcag cttctttccc tccggcgtct gccacctcaa
      901 aggctgaaca ggggttaatc tctagcgcgt gccgtttgat tccgtagtcg aatatgcggc
      961 gcgtccatct cagcacgtcc gttgctatgg tcggcgctcc acggtcaaca atacctttca
     1021 gcatgtcatc gatatggcgc ggtttcacgt cctctacctt catgctgcca atgcaggggt
     1081 ttatatcttt gtcgatacgg cggcggagta tatcggggtg cttccagcgc gggaggatct
     1141 gacgctcaaa gtattcagcg gcaagctctg aaacgcgcat agcgttcttc tctgcttcca
     1201 tcttcgccag tgcttcggtt ttgcgcttct gcttctctcc tgcaacgtca tagcccagcg
     1261 caacgcgagc cgatagctct ttggctgtct ctctggcctt tgatagtgat agctcgctat
     1321 acgaaccaat tagcatggcg cgggacttcc ctgcgagttt atagcggaat ctccatgtgg
     1381 gggttttgtc ggcttcacgg taacgtaggt ataaaccgtt accgtctgcc cgaccctcga
     1441 atcgctctcc gctcttaatc catgcgcgga tctgcatgtc tgttagtttt ggcattttgc
     1501 gaatatccaa actttgaata gggggtgtac acaaaggtgt acccaaacct cattgggtac
     1561 acctttgtgt acaccataat catgcgcttt gttgcgtctt tatgatagcc catgataaca
     1621 aaaaagcccg ctgggttgcg ggcttggtgt gatttatgcg tcgtcctaaa tgttgctgat
     1681 agcttactcg atattctgaa tctgctcgcg catctgctca atcaacactt tcagctcaat
     1741 cgcggagttt gtcacttcgg cattgataga cttcgacgca agagtgttcg actcgcggtt
     1801 gaattcctgc atcataaagt ccagacgacg accaacggct tcttttttct tcagaatgtt
     1861 gtaggtctct ttgacatgcg cttccaggcg gtccagttct tcggcaacgt caattcgttg
     1921 tgccagcaga accagctcct gctccagacg gttgttttcc aactgaacct gagcatcttc
     1981 cagcttcgcg accagacgct cacgctgcca ttgcaggatt tccggcatat gggcgcggac
     2041 tttgaccact tcggcggtga cgccttccag acgctgctcg atcaatgctt tcagtgcctg
     2101 accttcggtt tcacgcgcga caataaagtc gtccagcgta ccatccagcg ccgcgagaat
     2161 ttcagcggca atggcgtcaa gatcctgctc ctgggctgcc atcacgcccg gccagcgtag
     2221 aatatcaacc gggttgattt ccccttcgtc actctgcatt tttacccagt tcgcggcagt
     2281 taccagctgt ttagccagtt tttcgttgag gatcagctca ccttgcgcgc taatatctgg
     2341 ctcatagcgc agggtacatt ccactttacc gcgcgtcagg cgagaacgaa tacgctcgcg
     2401 aacgacaggt tcaaggctac ggaactgctc cggcagacga aagtaagttt ccagataacg
     2461 ctggtttacc gagcgcattt cccaggttgc gctcccccat tcacccttga tttcacgccg
     2521 ggcgtaggcg gtcatactgc ggatcataga cgttcctgtt tataaaagga gaggtggaag
     2581 gattatagcc atcgatgcct tgtcaggata ggaataaccg ccggaagtcc gtataatgcg
     2641 cagccacatt tgtttcaagc cggagatttc aatatgcgtc cagcaggccg tagcaataat
     2701 caggtgcgtc ccgttaccct gactcgtaac tatacaaaac atgcagaagg ctcggtgctg
     2761 gtcgaatttg gcgataccaa agtgttgtgt accgcctcta ttgaagaagg cgtgccgcgc
     2821 ttcctgaaag gtcagggcca gggctggatc accgcagagt acggcatgct gccacgttct
     2881 acccacaccc gtaacgctcg tgaagcggcg aaaggtaagc agggtggacg cacaatggaa
     2941 atccagcgtc tgatcgcccg tgctcttcgc gcggcagtag atttgaaagc gctgggtgag
     3001 ttcaccatta cgctggactg cgacgtgctt caggctgatg gtggcacgcg taccgcgtcg
     3061 attacgggtg cctgcgtggc gctggcagat gcgctacaga agctggtgga aaacggcaag
     3121 ctgaaaacca acccgatgaa agggatggta gccgcagttt ctgtcggaat tgtgaatggc
     3181 gaagcggttt gcgatctgga atacgttgaa gactctgccg cagagaccga catgaacgta
     3241 gtgatgaccg aagacgggcg catcattgaa gtgcagggga cggcagaagg cgagccgttc
     3301 acccatgaag agctactcac cttgttggct ctggcccgag ggggaatcga atccattgta
     3361 gcgacgcaga aggcggcgct ggcaaactga tttttaaggc gactgatgag tcgccttttt
     3421 tttgtctgta gaaaagtaag atgaggagcg aaggcatgaa accatatcag cgccagttta
     3481 ttgaatttgc gcttggcaag caggtgttaa agtttggcga gtttacgttg aagtccgggc
     3541 gcaaaagccc ctatttcttc aacgccgggc tgtttaatac cgggcgcgat ctggcactgt
     3601 taggccgttt ttacgctgaa gcgttggtgg attccggcct tgagttcgat ctgctgtttg
     3661 gccctgctta caaagggatc ccgattgcca ccactaccgc cgtggcgctg gcggagcatc
     3721 atgaccttga cctgccgtac tgctttaacc gcaaagaggc aaaagaccac ggtgaaggcg
     3781 gcaatctggt tggtagcgcg ttacaaggac gcgtaatgct ggtagatgat gtgatcaccg
     3841 ccggaacggc gattcgcgaa tcgatggaga ttattcaggc taatggcgcg acgcttgctg
     3901 gcgtgttgat ttcgcttgat cgtcaggaac gggggcgcgg cgagatttcg gcgattcagg
     3961 aagttgagcg tgattacaac tgcaaagtga tctctatcat caccctgaaa gacctgattg
     4021 cttacctgga agagaagccg gaaatggcgg aacatctggc ggcggttaag gcctatcgcg
     4081 aagagtttgg cgtttaaaga aactcgccgg atgaaaagtc atccggcgtc atattactgc
     4141 aactgtgccg caattagcgg ccagcgggcg tcaaaatcat ccgtcgggcg gtatttaaat
     4201 tcgctgcgga caaaacgtga cagcatacct tcacagaagg ccaggatctg gcttgccagc
     4261 agggtttcat cggtggtgta accttcaccc tcacgcattc tcttttcacg caatacctga
     4321 cgcagctgcg cttcaatacg ctcgaacagc tggttgatgc gcccttgcag gcgatcctgt
     4381 tcaaacatta gcgcatgacc agtgaggatg cgggtcaggc caggattacg ctcaccaaaa
     4441 ccgagaagca gcaacacaat cagacgcagg cgcgctgtgg tgtctttctc atctttcaga
     4501 atcaggttga tgcgagtaat caggctatct tcgataaact caatcaggct atcgaacatg
     4561 cgggtcttac tggggaagtg gcgatacagt gccgcttcgg aaacgccgac agaggcggcc
     4621 agttttgccg tcgtgatacg ttggcttcca tcgctggatt ccagcatcag cgccagagac
     4681 tgaagtattt cctcgcgacg gttccttttc gcagtttgtt tttctgccat gttacaaaat
     4741 acccctgaaa ataagcactt gccaggcggc acccacgcta tgaccgcaaa cgaaatgttt
     4801 gcggctatgt tatgacgtta ttcggatgcg tatgtgttac tgacgaccag agtgaccaaa
     4861 gccgccttca ccgcggtcgg tggcgtcgaa atcttccacc agattaaatt cagcctgtac
     4921 taccggaaca aaaatcatct gggcgatgcg ttcgccaggt tgaatggtga agctgtcctg
     4981 accacggttc cacacggaaa tcatcaactg gccctgatag tcggaatcga tcaatcctac
     5041 caggttacca agcacgatac cgtgcttatg tcccaatccg gagcgcggca gcatcattgc
     5101 cgccagtgaa ggatcggcaa tatgaatcgc cagcccggtc ggaaccagcg tagtgtcacc
     5161 cggagccagt tctacggcgt cgtcgagaca ggcacgcagg tcaagtccgg cagagccaga
     5221 ggtggcataa gtcgggagcg gaaattcctt cccaacgcgc gggtccagaa tcttaacgtc
     5281 gatttttttc atcataacgg gtcacgatct cgtcgagtaa tagttggcca aggagctctt
     5341 tgcgctcaag cggtaagact ttatctccgt cctgccagaa aaggtgtaat gcgttgttgt
     5401 cgctgttaaa tccttgagtt ggctgggaaa catcgttcgc gcagatcaga tcaaggtttt
     5461 tacggatacg tttttgccgg gcgtattctt ccacattatt tgtttcggcg gcaaatccaa
     5521 cgacgtaggg tcgatggtct tttagtgcgg caacgcctgc gacgatatcg gggtttttaa
     5581 ccatttttat tgttaattca tcaccctgcg tggcctgctt tttgattttt tctggggcca
     5641 cggtagctgc gcgataatcc gccacggcgg cgcagccgat aaaaatattt tgctgctgta
     5701 cagaagcatt cacggcggct tccatttcca gcgcggtcat cacatcaaca cgtttaacaa
     5761 acggtggcgt cggtagtgaa accggacctg ataccagcgt gacgttcgcg ccacgacggg
     5821 cagcggcggc ggcgatagca aaacccatct tgccggagct gtgattagag atataacgca
     5881 cgggatcgag cggttcacgc gtcggaccgg cggtaatcat aatgttcaga tgtttcaggt
     5941 cgttgacggg cgaaaaatgc gccaccgcca tatccacaat ggttaacgga tcgagcattc
     6001 gcccaggacc gatatcacca caagcctgac tgccactgtc tggcccccag atgagcaaac
     6061 cacgggaagc aagcccctct aaattatgct gcgtggcagc ggcacggtac atctgctggt
     6121 tcatggcggg gagcacggct acaggcgcag gtgtagccag acaaatcgtc gataccaggt
     6181 cattcgccat tccggcagca acacgggcaa tcaaatctgc cgtggcaggg gcgagaatca
     6241 ctaaatcagc ccatttaccc agctcaatat ggcccatagc ggcttcggct gccgggtcca
     6301 gcaaactgtc ggaaacggga taaccagaaa ccgcctgcaa gctaagtggg gtgataaagg
     6361 cttttgccgc ttcggtcatg gctacgcgga cgtcggcccc gcgatcgcgc aaacgacgca
     6421 ccagttcagg ggttttatag gcagcaatac cgccgctaac gccgagaacg atttttttac
     6481 cggccaggct catgatgatt tttcctgttg ggtgacacca gaagttggcg attttatcac
     6541 aatacttttg ttgtcgtgcc ttcacctgag aatcactttg cgaggcgctt tccaggattg
     6601 aaaactggcc gtcgatttaa cggaacggct atgacaggat gcgagcacca caaaggaggt
     6661 gaaggtgaaa aacaattcac agctgttgat gccgcgcgaa aaaatgctga agtttggtat
     6721 tagcgcctta acggatgtcg agctgctggc gctatttctg cgtaccggaa cgcgcggtaa
     6781 agatgtatta accctggcaa aagagatgct ggagaatttc ggctctcttt atggcttgtt
     6841 aacctctgaa tatgaacaat ttagtggcgt tcatggaatt ggcgtggcga aatttgccca
     6901 gttaaagggg attgctgaac tggcgcggcg ttactacaac gtgcggatgc gtgaagaaag
     6961 ccctttactc agcccggaga tgacgcggga atttttacaa agccagctta cgggtgagga
     7021 gcgggagatc tttatggtga tctttctcga ctcacaacac cgggttataa cgcatagccg
     7081 tcttttttcc ggcacgctaa accatgttga agtccatcct cgggaaatta tccgcgaagc
     7141 gataaaaata aacgcctcgg cgctgatcct tgcacataat cacccttcgg gttgtgctga
     7201 acccagtaaa gcggataaac tcattactga acggataata aagagttgtc agttcatgga
     7261 tttacgcgtg ctcgaccata tcgtgattgg gcgtggagag tatgtttctt ttgctgaacg
     7321 cggctggatt taacccgcta tgcgcgatcc ttcgggatct ttgtctgttc gggacttgag
     7381 cacatcgctg agtcagcgta tactacgcca cctttgagaa tctcgggttt ggcatttggg
     7441 cctggcaatc gagagttcac atagaactgc gatgactggg ctgtaaagcc tgacgaggcg
     7501 ccaatacccc atacgaagct cgagctaatt tgatttttgg agaatagaca tgtcccgagt
     7561 ctgccaagtt actggcaagc gtccggtgac cggtaacaac cgttcccacg cactgaacgc
     7621 gactaaacgc cgtttcctgc cgaacctgca ctctcaccgt ttctgggttg agagcgagaa
     7681 gcgttttgtc accctgcgcg tatctgctaa aggtatgcgt gtaatcgata aaaaaggcat
     7741 cgatacagtt ctggctgaac tgcgtgcccg tggcgaaaag tactaagtac ttagaggaaa
     7801 taaatcatgg ctaaaggtat tcgtgagaaa atcaagctgg tttcttctgc tggtactggt
     7861 cacttctata ccactacgaa gaacaaacgt actaagccgg aaaaactgga actgaaaaaa
     7921 ttcgatccag ttgttcgcca gcacgtgatc tacaaagaag cgaaaatcaa ataattctcg
     7981 ctttgatgta acaaaaaacc tcgctccggc ggggtttttt gttatctgct tgcccccata
     8041 ttgactgcat ctgttcattc ctggagatgc tatgcctgaa ttacccgaag ttgaaaccag
     8101 ccgccgcggc atagaaccgc atctcgttgg tgcaaccatt cttcatgcag tggtgcgcaa
     8161 cggacgcttg cgctggccgg tttcagaaga gatctaccgt ttaagcgacc aaccagtgct
     8221 tagcgtgcag cggcgggcta aatatctgct gctggagctg cctgagggct ggattatcat
     8281 tcatttaggg atgtctggca gcctgcgcat ccttccagaa gaacttcccc ctgaaaagca
     8341 tgaccatgtg gatttggtga tgagcaacgg caaagtgctg cgctacaccg atccgcgccg
     8401 ctttggtgcc tggctgtgga ccaaagagct ggaagggcat aatgtgctgg cccatcttgg
     8461 accggagccg cttagcgacg atttcaatgg tgagtatctg catcagaagt gcgcgaagaa
     8521 aaaaacggcg attaaaccgt ggctgatgga taacaagctg gtggtagggg tagggaatat
     8581 ctatgccagc gaatcactgt ttgcggcggg gatccatccg gatcggctgg cgtcatcact
     8641 gtcgctggca gagtgtgaat tgttagctcg ggtgattaaa gcggtgttgc tgcgttcgat
     8701 tgagcagggt ggtacaacgc tgaaagattt tctgcaaagt gatggtaaac cgggctattt
     8761 cgctcaggaa ttgcaggttt acgggcgaaa aggtgagccg tgtcgggtgt gcggtacgcc
     8821 gattgtggcg actaaacatg cgcagcgggc aacgttttat tgtcggcagt gccagaagta
     8881 attcatgcac gccggatcgc ataccatccg gcataaacgc tacgctaact tcgccatcag
     8941 cgcctgatgg acattctccg gcaggaaatg ggtgacatcg ccctgatggc gcgccacctc
     9001 tttcaccaac gatgaagaga taaacgacca ctctttcgac ggcatcagaa acacactttc
     9061 cagttccggc attaagtggc gattcatatg cgccagctgc atttcatatt caaaatccgc
     9121 caccgcacgc aggccacgaa tcagcaccgt agcgtgttga ttacgggcga agttcgccat
     9181 caaatcacta aacccaacta cttccacgtt ccccagatgc gcggttgcct gctgtgccag
     9241 cgccacacgc tcttccaggg taaacatcgg ttttttactg gggctggcgg caatcgccag
     9301 aataacgtga tcgaacattt gcgtggcgcg cgtcacgata tcgatatgac cattggtaat
     9361 gggatcgaaa gtacccggat aaatcgcccg tttttgcata acaacctcaa tgcgttttcg
     9421 gtggcaggta aggttccagc agttgaagca ggcgctgtag cgcgccctgg ttttgataca
     9481 gtacttcaac ggcatgacgg ccatagaaac tacggtaatc ggcgtcggtg agtaaagagg
     9541 aaacctcttt tgcaagcgta gtggcatcgg taacggtaat cagcccgctt gcctgctcca
     9601 gccgcgcgca aatgtcttta aaattaaaag tatgcggccc catcaatacc ggaatagcgt
     9661 gtgcggcagc ttccagcgga ttatgcccac cacgttcaac cagtgaaccg ccaacaaagg
     9721 cgagatcggc aatgccgtac agtaacatca actcgcccat cgtatcgcca accacaacct
     9781 gcgtgctggt ggaggggact tcccctgaag agcgtgtgat atagcttagt ccagcctggc
     9841 ggacaaggtt aatcgcatcc gggaagcgtt ctggatgacg gggtaccagg atgagcaata
     9901 aattcgggaa ttgctgtaac aatacctgat gtgcggcgat caccacactc tcttcacctt
     9961 cgtgagtgct ggtggcaatc cataccgggc ggtgtggtgc ccactggcgg cgcagcgtca
    10021 cggctttagc agccaactgc ggcgttacag aaatatcgaa tttcaggcta ccggtaacgg
    10081 ttacctggtt attttttgcg cccagcgcca caaaacgtgc accatcttct tcattttggg
    10141 cggcaatcag cgtaatacga cgcagcaagc gacggacgaa tttacccagt ttggcataac
    10201 ctgcggccga gcgggcagag agtcgcgcgt tcgcgatcac cagcggaatt ttacgtttat
    10261 gtagcgccgc aatcaggtta ggccatagtt cggtttccat aatcaacacc agtttagggt
    10321 cgactttatt caggaaacga ttaagtgcat cgggcaggtc atacggcaga taaacgtgct
    10381 gaacatcctt cccgaaagcc gattgtacgc gctccgaacc ggttggcgtc atggttgtaa
    10441 cggtaatcgg taaatcagga taacgatgac gcagcgcgcg caccaacggg atcgccgcca
    10501 gagtttcacc gacggagacg gagtgcagca taatgccgcc tggttttagc agatggcggt
    10561 aaaaaccgta acgttcaccc cagcgttttc gataggccgg agccttacgt ccgcgcaccc
    10621 agagccgtat ccagatcagc ggctgaataa ggtagagaag ggcggtgtaa agcaattcga
    10681 gcatagtaaa tagctgactt atggatgtgc tggggattct atgtatttag ctgtggcttt
    10741 accattactt ttcccgtttt tgacttaaat agcaatttac ccgccaattc ttgctgaaca
    10801 tctatacttt ttttgtattt ttatgcgatt cattgatttc tgctgtacgt gcaggaagga
    10861 atcgcagagc aacagtgaaa gaatatggtt gctaatgatt ttatttgatt gatttcttta
    10921 ttgaaaatca acaatgtgct taaaaatcat tcgggtcgga ttgccgcgaa agaagtgata
    10981 cactagcgcg tcaatatcag tgcggtatca gtattcaggt agctgttgag cctggggcgg
    11041 tagcgtgctt tttctgcttc accttaatcg gataatctca acaaaaagag ttacttgtgg
    11101 ataagctatt tcgaagaatt ttgctcatta agatgcgttt tcatggggat atgttattaa
    11161 ctactcccgt cattagttcg ctgaaaaaaa attaccctga cgcaaaaatc gatgtgctgc
    11221 tttatcagga caccatcccg atcctgtctg aaaatccaga gattaacgcg ctctacggca
    11281 taaaaaataa aaaagcaaaa gcctcagaaa aaattgccaa cttttttcat ctcatcaagg
    11341 tattacgtgc caataagtat gaccttatcg tcaatctcac cgatcaatgg atggttgcta
    11401 tactggttcg cttattaaat gcccgtgtga aaatttccca ggattatcat catcggcagt
    11461 ctgctttttg gcgtaaaagt ttcacccatt tggtgccgtt gcagggtgga aatgtggtgg
    11521 aaagtaactt atccgtgctg accccattgg gagttgattc gttggtgaag cagacaacca
    11581 tgagttaccc gcctgcaagc tggaaacgta tgcgtcgcga acttgatcac gctggtgttg
    11641 gacaaaatta tgtggttatc caacctacgg cgcggcaaat cttcaaatgc tgggacaacg
    11701 ccaagttttc cgctgtgatt gatgccttac atgctcgtgg ttatgaagtt gttctgacgt
    11761 ccggcccaga taaagacgat ctggcctgcg tcaatgaaat tgcgcaggga tgccagacgc
    11821 caccagtaac ggcgctggct ggaaaggtga ccttcccgga acttggtgcg ttaatcgatc
    11881 atgcgcagct gtttattggc gttgattccg caccggcgca tattgccgct gcagttaata
    11941 cgccgctgat atcgctgttt ggtgcgacag accatatttt ctggcgtccc tggtcaaata
    12001 acatgattca attctgggcg ggagattacc gggaaatgcc aacgcgcgat cagcgtgacc
    12061 gaaatgagat gtatctttcg gttattccgg cggcagatgt cattgctgct gtcgataaat
    12121 tactgccctc ctccacgaca ggtacgtcgt tatgatcgtt gctttttgtt tatataaata
    12181 ttttcccttt ggcggtttgc agcgcgattt tatgcgtatt gctcagacag tcgccgcccg
    12241 aggtcatcat gttcgggttt atacccagtc gtgggaaggc gaataccctg atgtatttga
    12301 actgatcaaa gtgccggtta aatcgcatac caatcacggg cgcaatgcgg agtattttgc
    12361 ctgggtgcaa aaacatttac gcgaacatcc cgtcgataaa gtcgttggat tcaacaaaat
    12421 gccggggctg gacgtttatt atgccgctga tgtttgttat gccgagaaag tcgcgcagga
    12481 aaaaggcttt ttctatcacc tgacgtcacg ttatcgccat tatgccgcct ttgagcgggc
    12541 aaccttcgaa cagggcaagc cgacacagct gctgatgctg acagataagc aaatcgccga
    12601 tttccagaaa cattatcaga ctgaagcgga gcgttttcat attctgccac cggggattta
    12661 tcctgatcgt aaatatagcc agcagccagc taatagccgt gaaatcttcc gtaagaagaa
    12721 tggaataacc gaacaacaat atttattgtt gcaggtcggt tcagacttca cgcgtaaagg
    12781 tgtcgatcgt tccattgaag cacttgcttc gttaccggat tcgctgcgcc acaacacatt
    12841 gctatatgtt gttggacagg ataaaccgcg aaaatttgag gcgctggcag aaaaacgcgg
    12901 cgtgcgcagt aatgttcact tcttctcggg gcgcaacgat gtctcggaat taatggcggc
    12961 ggcggattta ttactgcatc ctgcctacca ggaagcagcg ggaattgtgc tactggaagc
    13021 gattactgca ggattaccgg tactaacaac agccgtttgt ggctatgcgc attatattgt
    13081 cgacgccaat tgcggcgagg ctattgctga gccattccgc caggaaacat tgaatgagat
    13141 tttacgcaaa gcgttaacgc aatcttcatt gcgccaggcc tgggcggaaa atgcgcgaca
    13201 ttatgctgat acacaagatt tatacagtct gccagagaaa gcggcggata tcataacggg
    13261 tggtctggat ggttgaactt aaagagccgt ttgccacgtt atggcgcggt aaagatcctt
    13321 ttgaggaagt taaaaccttg cagggtgagg tttttcgtga actggaaact cgccgcactc
    13381 tgcgatttga aatggcgggc aaaagctatt ttctcaaatg gcatcgcggc acgaccctga
    13441 aagagataat caaaaattta ctctcattgc ggatgccagt attaggcgca gaccgcgaat
    13501 ggagtgcgat tcatcgactg cgggatgtcg gcgttgatac tatgtatggg gtggcatttg
    13561 gcgaaaaagg tattaatccg ctgagcagaa cctcgtttat tattaccgaa gatctgaccc
    13621 caaccatcag tctggaagat tactgtgctg actgggcgac caacccacca gatgttcgcg
    13681 taaagcgtat gcttattaag cgtgtcgcga cgatggtgcg cgatatgcat gctgcgggca
    13741 ttaaccaccg tgactgttat atctgtcatt tcctgctgca cttgcctttt tccggtaagg
    13801 aagaggagtt aaaaatttcg gtaattgacc tacaccgggc gcagcttcgc acgcgcgttc
    13861 cacgtcgttg gcgcgataaa gatcttatag ggctttattt ttcttcaatg aatattggcc
    13921 tgactcagcg ggatatctgg cggtttatga aagtgtattt tgccgccccg cttaaagaca
    13981 ttctcaagca ggaacaagga ctgctgtcgc aagcagaagc aaaagccaca aaaatcaggg
    14041 aaagaacgat tcgaaaatcg ttgtaatttc cggagagaaa tatgagtgcc cactatttta
    14101 atccacaaga gatgatcaat aagacaatca tcttcgatga aaggccagcg gcgtcagtgg
    14161 catcatcatt ccatgttgct tatggcattg ataaaaactt tctttttggt tgtggtgttt
    14221 caatcacgtc agttttgtta cataacaacg acgtgagttt tgttttccac gtttttattg
    14281 atgatatccc tgaagccgat atccagcgtt tagcccaatt ggcgaaaagc tatcgtacct
    14341 gtatccagat ccatctagta aattgtgaac ggcttaaggc attaccgacg accaaaaatt
    14401 ggtctattgc catgtatttc cgttttgtaa ttgcagatta ctttattgat caacaagata
    14461 agatccttta cctggatgct gatatcgcct gtcagggaaa cttaaagccg ctgataacaa
    14521 tggatcttgc caataacgtt gctgctgttg ttactgaacg cgatgctaac tggtggtcgt
    14581 tacggggtca aagtctgcag tgtaatgaac ttgaaaaggg ctactttaat tcaggtgtcc
    14641 tgttaattaa tacactagcg tgggcgcagg agtccgtttc tgctaaagcg atgtcgatgc
    14701 ttgctgataa agccatcgtt tcccgtttca cctatatgga tcaagatatc cttaatctta
    14761 tcctgttagg gaaagttaaa ttcattgatg ctaaatacaa tacgcaattt agtttaaatt
    14821 atgaattaaa aaaatcattt gtttgtccaa ttaatgatga aaccgtatta attcattatg
    14881 tcggcccgac aaaaccctgg cattactggg ccggttatcc aagtgcgcaa ccttttatca
    14941 aagccaaaga agcatcgccc tggaaaaatg aaccgttaat gcggccagtt aactcaaact
    15001 atgctcgtta ttgcgccaag cataatttta aacaaaataa accaattaac gggataatga
    15061 attatattta ttatttttat ttaaagataa taaaatgaaa ttgagaaaag ttaaatgaat
    15121 gaatttataa aagaacggtt ttcgtattta gcagataata aaaaagaaaa cgtcccagag
    15181 ctaaatgttt cctacggtat cgataagaat tttttgtatg gtgctggcgt ttcaatttct
    15241 tccgttttga ttaataattc agatattaat tttgtctttc atgttttcac tgattatgtg
    15301 gatgatgatt atttaaagtc atttaatgaa acagcaaaac aatttaatac ctcaattatt
    15361 gtatatttaa ttgaccccaa atactttgct gatctgccga cgtcacagtt ttggtcatac
    15421 gcgacatact tcagggtatt gtcttttgaa tatctgagtg aaagtatttc cacactgctg
    15481 tatctggatg ccgatgttgt ttgtaaagga agcctgaaac ctctcacaga aattatattt
    15541 aaagatgagt ttgctgcggt cattcctgac aatgatagta ctcaggcggc atgtgcaaaa
    15601 cgcctcaaca ttcccgaaat gaatggacgt tatttcaatg caggcgttat ctatgtcaat
    15661 cttaaaaaat ggcatgaagc aaatttgaca ccgtatttac tcaaactttt acgaggggaa
    15721 actaaatatg gctctcttaa atatttagat caggatgcgt tgaatatcgc atttaatatg
    15781 aataatatct accttgcgaa ggattttgat actatttata ccctgaaaaa cgaactttat
    15841 gatcgtagtc atcgaaagta tcagcaaacc attaccgata aaacagtgtt gattcactat
    15901 acagggataa ctaaaccatg gcatagctgg gctggatatc cgtctgcatc atactttaat
    15961 atcgcgcgtg aacaatctcc ctggaagaaa tatcctctta aagaggcgcg gactgttgca
    16021 gaaatgcaga aacaatataa gcatctgttt gcccatggtg agtatataaa aggcataact
    16081 tcattaatta agtacaagct taagaaataa attactctta tgattacaag tatacgttat
    16141 cgcggcttct cattttatta taaagataat gataataaat ataaagaaat ctttgatgaa
    16201 atactggcct ataattttaa aacagtaaaa gtattatgta atattgatga taccaaagta
    16261 tcgttgattg atacgaaata tggtcgctat gtttttaaag tctttgctcc gaaaacgaaa
    16321 aggaatgaac gttttttaaa atcttttgta aaaggtgact actatcagaa tttgatcgtt
    16381 gaaaccgatc gtgtcagaag tgcaggtctt acttttccta atgattttta ttttctggct
    16441 gagcgtaaaa ttttcaacta tgccagtgtt tttatcatgc ttatcgagta tgtcgagggc
    16501 gtagagctca atgatatgcc gattattcca gaaaatgtta aagcagaaat taaggcttca
    16561 atggaaaaat tgcatgcctt aaatatgcta tcgggcgacc cacaccgggg taactttatc
    16621 gttagtaaag atggtgtcag gataattgac ctatccggta aaagctgtac ggcagagcgt
    16681 aaagctcgtg atcgtctggc catggaacgt cacctgggta ttgccaacga aataaaagat
    16741 tatggctact attcagtaat ctacagaacc aaactacgca aatttattaa aaaattaaaa
    16801 ggcaaagcgt aaaccacaca gtcaaaacgg aaccaacatg gatttattag ctgagagtat
    16861 tactgaagtc gctgtctctg gggaaattgc taacaccgat cgtgtgttaa atatcgctta
    16921 cggtattgac cgtaactttt tatttggtgc ggcagtatct atgcaatcag ttgttatgca
    16981 taacccggac cttgcggtta agtttcatct ctttaccgac tacattgatg aagattatct
    17041 acaacgtgtt aatgctttta ccagcaaaaa tgctaacgtt gaagtaataa tttataaagt
    17101 ctccaatgcc tttattgata tcttccccag cctgaaacag tggtcttatg caacattctt
    17161 ccgtttagtt gcgttccagt atttgagtga aactattgaa aatctgttat atatcgatgc
    17221 tgatgtcatc tgtaaaggct cattagctgg attgcttgat attaattttg atgaggataa
    17281 gttcgcagct gttattaaag atgtgccttt tatgcaggaa aaaccagcga agcgtctggc
    17341 tatagaggga cttccaggga attatttcaa cgccggtgta gtatatctgc agcttgaagc
    17401 atgggcgaaa aatgatttta tgaataaagc cattgctatg ctggcaagtg acccgcagca
    17461 cacgaagtat aaatgccttg atcaggatat tttaaatatt ctgttctttg gtcattgtaa
    17521 ttttattagc ggcgattatg attgctttta tggcattgac tatgagttaa aaaataaaag
    17581 cgatgaagat tataaaaaga ccattaccga tgatactaag ctgattcatt atgttggcgt
    17641 aacgaagccc tggaacgact ggacgaatta tccctgccag aagtatttta atgaggctta
    17701 tcaggattct tgctggaatg atgtggcgtt tattccagcc acgaatgaaa agcagtatca
    17761 agtgaaatat caacatgcaa agaaaaatgg tgatacgttt aacgctttta tttacttcat
    17821 taaatttaaa ttaaataagt ataaaagaaa actatttgga taataataaa aatcatggat
    17881 gtctgagtta caataataat taaggatgag tactatcata ttttattagt tcaggcaaga
    17941 gaacacgatg agcaatgatt accctttagt atccataata ataccgacgt ataattcatc
    18001 tgattacatt actgaaactc taacgaaatt agaaaaacaa acttacccaa attttgaaat
    18061 tgttattgtt aatgatggtt ctaaagataa cacatcaaac gttttgagag agtatgggtt
    18121 aacccactct cgattaatta ttatcaataa agaaaatggc ggtgtttcgt ctgccaggaa
    18181 tacaggtatc cgcaaggcgc aaggacagtt tatatgtttt atggatgatg atgatgagat
    18241 agatcctaac tatctgctga agatgtatac cagacaacat gagacgggag gggatgcgat
    18301 ttattgtggg ctttatggcc attatataaa agatggtgtt acttactcac ctataaatac
    18361 agagtttaat gaaggatctt tacttttcga ctttttttat aaaaaggtta gattccatat
    18421 agggtgcttg tttataagaa aacaacttct ggaagagaat aatctttttt ttgatgaaga
    18481 tttacgacta ggagaagatc ttgattttat ctatcgactg ctaattacat gcgatatgta
    18541 tgcggttcca tattatatgt ataagcataa ctatagagaa aattccttaa tgaacacatg
    18601 tagaaccatc actcattatc gacatgagtc atttgcgcac gaaagaatct actcttctgt
    18661 gatgcagtta tataaaggta accggaaaga agaaattcat acattattga gtcaaaatag
    18721 agcttatcat aaaactcgtt atttatggaa tgttctactt aatggtgatt ttaaactatt
    18781 gaatcaatta gttgaaagca atgaaaaaga attaagagat tgtaatcttc ctggcaagag
    18841 agataagaga cgagcaaaaa tattagcatc aaaaaattat attctctgga ggttggtaag
    18901 actggtaaat agaaaaaaga ataaacgtta gatcttgcat taaataaccc atcctcgtag
    18961 cataggttga aattatatgt cgttttgttg gaatgaaatt aattctggta taaagtcttt
    19021 aattctcata ttatgtattt tttctttaat gactttgtct ttatgggatg atgttgcaac
    19081 aaagtttctt catgcagctg gaattatatc tgcattgtat tttcttgcga caccaaaaaa
    19141 aacaataact aataatccta ctttgttaat tttcatctca ttatgtcttt tgggtatcgt
    19201 aaatatcatc tggtattcac attataaagt ttcaggctct gtttatacca atgcatatcg
    19261 tggcccaatg gaaactggaa aaattgcctt gtgtagcgct tttattttct tagttctttt
    19321 tgctaaaaat gagatgagaa caaaaataaa atttgggaaa ctaattctgt tcgcatccct
    19381 ggcaacgcag ttactttttt ttgcgcatgc catgtggcaa catttctatt taaacgtcga
    19441 ccgtgttgca ttatcagctt cccacgctac aacagcaggc tacatcatcc tttttccttc
    19501 tttactggca tcaattctca ttttaaaatc cgactttaga cataaaacaa cattatatac
    19561 aattaacttc atgcttagct tatgtgctgt catagtaact gagacgcgtg cagccatatt
    19621 agtgtttcca ttctttgcgt taatattaat cgtaatggat agttatatta ataagcgaat
    19681 taattataag ttatattgtt ttattgcgat tgcattatta gcaggtgtat tttcttttaa
    19741 agatacattg cttatgagaa tgaatgactt aaataacgat ttagttaatt attcgcatga
    19801 taacaccaga acttcagtcg gtgcccgttt ggcaatgtat gaagttggct taaaaacata
    19861 ttctccaata ggacaatcac tggaaaaacg tgcagaaaaa atacatgagc tagaagaaaa
    19921 agagcctaga ttgagtggcg ctttaccctt tgtagattct catttgcata acgatctcat
    19981 agatacgtta tcaacgcgtg gtattcctgg agttgtatta acaattttag cattttcagc
    20041 aatactcata tatgccttaa gaactgctaa agaaccttat attttaatct tgcttttttc
    20101 actactggta gtaggcctaa gtgatgtaat actcttttct aaaccggttc cgactgctgt
    20161 gtttgtcacc ataatattgc tttgtgctta ttttaaagca caatcagacc aatatttatt
    20221 agataagtaa tgaatatatg aaataaaatc agcaaggagg ctgattttat ttcaaaatta
    20281 tctattaagg ggttgtaatt taaatcatag cagcgttttc agcgttttct tcaatgaatc
    20341 gcttaacagc atttgctgtc aattgagaca actcattccc cggagcccta caaaccatct
    20401 gattcttccc atacccacca attaatcccg gatcggttgg tccataaacc gtgatattgg
    20461 gtctatccag tgccgccgtt aaatggctta accccgtatc caccgacact acaaatttag
    20521 ccccggccag cacgcgggca acgccttcca gactcatctt cggcaatact tcaacataat
    20581 caaatccttc cgccagtcgt ttcgcccgtt cttcctcatg cggcgcgccc cacggaagtt
    20641 taatccgtat tcctgaatca gccagtaaac caatcaattc tcgccagtgt tcttccggcc
    20701 agtgtttatc atcacgggtc gtcgcatgaa gaaatacggc atattcgcca gcatctgtag
    20761 gcaggttcgt cagaaaatgc tgtgcgatag cataatcgcc ctgggtttgc ggtttgctat
    20821 agcccaaact tttggcaaac agttcgcggg tgcgttctac ggcgtgctgc tgttttgcaa
    20881 tatgatgctt acaattgtaa aacaggctgg ctaaaggttc gcgagcggtt tgccagtcca
    20941 atccatgctt tacgccatgc gccagacgtg tcaccagtgc cgcgcttttt accagcccct
    21001 gagcgtcgat aactgcgtca tagttctctg cttgtagcgc ttcacgaaac gctttgcgtt
    21061 cagcttttat gggggccgag aaccaggctt tacgccagcg acgtattgcc acaggaataa
    21121 ctcgctcaac ggcagcgtgc caggaaggaa tctgtgcgaa cccttcttcc accacccagt
    21181 caaacttaat ccctgggatt gcctgctggg catcagtgag tgcgggcaac gtatggagaa
    21241 catcgcccat cgacgatgtt ttaacgatca aaacccgcat ccgtcaggct tcctcttgta
    21301 acaatagcgt gttgagttct tccagtacgc gctggggagt aatgtcgatc aagctctggt
    21361 gataaccctc cgcagcgtca cctttacgca ctttgtgata gccggtaatc agacggatca
    21421 cgcgcgcttt atgggatagc ggcggtgtga agtccgggct acttggacca tacagggcaa
    21481 ccagcggacg attgagcgcc gccgcaacgt gcatcaggcc agaatcgtta gtgacaatgg
    21541 ctttacaggc tgcaatcagg ataaccgctt gatcaagctg tgtttccccc gccaggttcc
    21601 gacaccatgc ctgctgctcg gtattcaaag cggcaagaat ctcattgccc gcttcatgat
    21661 ctttcgcaga gccaaacaga accacctgat aaccttcatc aatcagctgc tttgccagtt
    21721 ccgcatagtg gtagtgtggc cagcgttttg ccggaccaaa ctccgcaccc gggcaaaagc
    21781 caatcatcgg acgttctgat gaaagcgaaa attgattaca ggtatatgat ttttcacctt
    21841 cgctcacctg caactgcggc cataacaatg gctgcggcag atcttgtgcg gtacgcataa
    21901 tgcctttgtc ataggccagc gcgacatagc gttccaccat tagcggccag gcttctttat
    21961 cgagcacgcg tacatcgttg agtaaaccgt agcgcatctc gccgcgccag ccggtgcgat
    22021 gaggaatacc cgcgaagaaa ggcactaatg cagatttgaa ggagttgggt aagacgtagg
    22081 cgcggtcgta gcgcttttca cgcaggctat gacccagttt gcggcgttcg ccgatttcca
    22141 gcgctccgtg accgagaggc atagggatag cttcgttaac ttccggcatc cgcgataata
    22201 atggacggca ccatgccggt gccatcacat cgattatcgc ctggggatag cgcgcctgga
    22261 gcgtgcgata gagactttgc gacatcatca tgtcgccaac ccaagacggg ccgatcacca
    22321 gtattttcat gcagagctct tatgcgtcgc gattcagcca ggccatgtat tccgttacac
    22381 cttcagcaac ggttttgaac ggtttgtcgt aacccgccgc gcgcagattt gtcagatctg
    22441 cctgagtgaa cgcctggtag cggcctttca gcttatccgg gaacggaatg tattcgatct
    22501 ggcctttctt gtgataagcc agagtagcat ctgctacggc ctggaaggat tccgcacgac
    22561 cagtaccgag gttgaagata ccggaaacgc cattttccag gaaccacagg tttacatctg
    22621 ccacgtcgcc cacatagacg aagtcacgtt tgaagttctc gctaccttca aacagcttcg
    22681 gtgattcacc gttgttaagc tgagtgttga gatggaaagc gacgctcgcc atgctgcctt
    22741 tatggccttc acgcggtcca taaacgttga aatagcggaa gccaacaatc tgcgagttcg
    22801 cttcaggcag gatttggcga acatattcat caaacaggaa ttttgagtaa ccgtagacgt
    22861 tcaacggttt ttcgtactcg cgggattcaa taaagtcgga ggtgcgtccg ccgtaggtgg
    22921 ctgcggaaga agcgtacagg aacgggattt cgcgctccag gcagtagtgc agcagctcct
    22981 tggagtattg atagttgtta tccatcatat acttgccgtc ccactcggtg gtggaagagc
    23041 atgcgccttc gtggaaaatc gcttcgacat cgccgaactc ttcgccagcc ataatctgga
    23101 tcaggaagtc ttccttatcc atatagtccg cgatatccag atccaccagg ttcacaaact
    23161 tggtgccgtc tttcaggttg tccaccacca gaatatcggt gatgccttta tcattcaggg
    23221 ccttaacgat gttgctgccg ataaagcccg cgccgccggt aacgatgatc ataactgtaa
    23281 ccttcgaatt atggagtcag agacaatctc agacacgaat acttctatca tatcactata
    23341 cggcgttggc ttcagccatt cgccaaaagt gagattgatg gacgttattt atcctgaatt
    23401 ttgcagaagt gttaacgcgt catctcgtcg cgacgtataa gtttgggtaa tatgtgctgg
    23461 aatttgccct gtctggagaa tcgcaatgcg tggagaattt tatcagcagt taaccaacga
    23521 tctggaaacc gcacgggcgg aagggttgtt taaagaagag cgcattatta cgtctgcgca
    23581 gcaagcagat atcactgtgg ctgatggaag ccacgtcatt aacttttgtg ccaacaacta
    23641 tctcgggctg gcgaatcatc cggatctgat tgcggcagca aaggcgggaa tggattctca
    23701 cggtttcggc atggcttcgg tgcgttttat ttgcggcact caggacagcc ataaagagct
    23761 tgaacaaaaa ctggcggcat tcctggggat ggaagatgcg attctctact cttcctgctt
    23821 tgatgctaac ggtggactgt ttgaaacgct gctgggtgcg gaagacgcta ttatctccga
    23881 cgcactgaac cacgcgtcta ttattgatgg tgtgcgtctg tgcaaagcta aacgctatcg
    23941 ctatgccaac aacgatatgc aggagctgga agcacgtctg aaagaagcgc gtgaagccgg
    24001 tgcgcgtcat gtgctgattg ccaccgatgg tgtgttctca atggacggcg tgattgccaa
    24061 cctgaaaggc gtttgcgatc tggcagataa atacgatgcc ttggtgatgg tggatgactc
    24121 ccacgcggtc ggttttgtcg gtgaaaatgg tcgtggttcc catgaatact gcgatgtgat
    24181 gggccgggtc gatattatca ccggtacgct tggtaaagcg ctgggcgggg cttctggtgg
    24241 ttataccgcg gcgcgcaaag aggtggttga gtggctgcgc cagcgttctc gtccgtacct
    24301 gttctccaac tcgctggcac cggccattgt tgccgcgtcc atcaaagtac tggagatggt
    24361 cgaagcgggc agcgaactgc gtgaccgtct gtgggcgaac gcgcgtcagt tccgtgagca
    24421 aatgtcggcg gcgggcttta ccctggcggg agccgatcac gccattattc cggtcatgct
    24481 tggtgatgcg gtagtggcgc agaaatttgc ccgagagctg caaaaagagg gcatttacgt
    24541 caccggtttc ttctatccgg tcgttccgaa aggtcaggcg cgtattcgta cccagatgtc
    24601 tgcggcgcat acccctgagc aaattacgcg tgcagtagaa gcattcacgc gtattggtaa
    24661 acaactgggc gttatcgcct gaggatgtga gatgaaagcg ttatccaaac tgaaagcgga
    24721 agagggcatc tggatggccg acgtccctgt accggaactc gggcataacg atctgctgat
    24781 taaaatccgt aaaacagcca tctgcgggac tgacgttcac atctataact gggatgagtg
    24841 gtcgcaaaaa accatcccgg tgccgatggt cgtaggccat gaatatgtcg gtgaagtggt
    24901 aggtattggt caggaagtga aaggcttcaa aatcggcgat cgcgtttctg gcgaaggcca
    24961 tatcacctgc ggtcactgtc gcaactgtcg tggcggtcgt acccatctgt gccgcaacac
    25021 gatcggcgtc ggcgttaacc gtccgggctg cttcgccgaa tatctggtga tcccggcgtt
    25081 caacgccttc aaaatcccag acaatatctc cgacgacctg gcttccattt ttgatccctt
    25141 cggtaacgcc gtgcataccg cgctgtcgtt cgatctggtt ggcgaggatg tgctggtctc
    25201 tggtgcaggt ccgataggta ttatggctgc ggcggtggcg aaacacgttg gtgcacgcaa
    25261 tgtggtgatc actgatgtta acgaataccg ccttgagctg gcgcgcaaaa tgggtatcac
    25321 ccgtgcggtt aacgtcgcga aagaaaacct taatgatgtg atggctgaac tgggcatgac
    25381 cgaaggcttt gatgtcggtc tggaaatgtc cggtgcgccg ccagcgtttc gtaccatgct
    25441 tgacaccatg aaccacggcg gccgtattgc gatgctgggt attccgccgt ctgatatgtc
    25501 tatcgactgg accaaagtga tctttaaagg cttgttcatt aaaggtattt acggtcgtga
    25561 gatgtttgaa acctggtaca agatggcggc gctgattcag tctggcctcg atctctcgcc
    25621 gatcattacc catcgtttct ctatcgatga tttccagaag ggctttgacg ctatgcgttc
    25681 gggccagtcc gggaaagtaa ttctgagctg ggattaacac gaacaagggc tggtattcca
    25741 gcccttttat ctggggataa tctgttgaat atgtaaaatc ctgtcagtgt aataaagagt
    25801 tcgtaattgc gctgatctct tatatagctg ttctcattat ctctctaccc tgaagtgact
    25861 ctctcacctg taaaaataat atctcacagg cttaatagtt tcttaataca aagcctgtaa
    25921 aacgtcagga taacttcaga ggtcgtcggt aatttatgat gaacagcacc aataaactta
    25981 gtgttattat tccgttatat aatgcgggcg atgatttccg cacttgtatg gaatctttaa
    26041 ttacgcaaac ctggactgct ctggaaatca ttattattaa cgatggttca acggataatt
    26101 ctgttgaaat agcaaagcat tacgcagaaa actatccgca cgttcgtttg ttgcatcagg
    26161 cgaatgctgg cgcatcggta gcgcgtaatc gtgggatcga ggtggcgacg ggcaaatatg
    26221 tcgcttttgt cgatgctgac gatgaggtct atcccaccat gtacgaaacg ctgatgacca
    26281 tggcgttaga ggacgacctc gacgtggcgc agtgcaacgc tgactggtct tttcgtgaga
    26341 cgggagaaac ctggcaatcc atccccagcg atcgccttcg ctcaaccggc gtattaaccg
    26401 gcccggactg gctgcggatg gggctttctt cgcgccgttg gactcacgtt gtctggatgg
    26461 gggtttatcg ccgtgatgtt attgttaaaa ataacattaa atttattgcc ggattacatc
    26521 atcaggatat tgtctggaca acagaattca tgtttaacgc gctgcgtgcg cgatataccg
    26581 agcaatcatt atataaatat tatctgcata atacgtcagt gagtcggttg catagacaag
    26641 ggaataaaaa ccttaattat caacgtcact atattaagat tacccgcctg ctggagaaat
    26701 taaatcgaaa ttatgccgac aaaattacga tttatccgga atttcatcag caaataactt
    26761 acgaagcatt gcgtgtttgc catgcggtgc gcaaagagcc ggatattctt acccgccaac
    26821 ggatgattgc cgagatattt acttccggta tgtataagcg cctgattacc aatgtgcgca
    26881 gcgtgaaggt cggttaccag gcgttactgt ggtctttccg cttatggcaa tggcgcgaca
    26941 aaacgcggtc gcaccatcgc attacgcgta gcgcctttaa tttgcgctag cgttaaactt
    27001 cgccgcttcg ggctgtttgc cccagccttg ccactgatgc tggaagtaaa cgatcagcgt
    27061 gctctggctg atgctttcgc ttaatacttc aaagaagcga ttagcataaa caggttctat
    27121 cggtttcttc ggtttgcaca gcttcacgcc acggaacgga ttacgcggtg catcaggcac
    27181 agcgtttttc ggcggtgtag aagtatcaac ctgcggttca ttcagcaagc tgctggcttt
    27241 caccagcgta atgtctggcg gcaggttata aaccatctgt tgtagcacgc gtaccgttaa
    27301 agggtgagga tgcccaatgg caatggttga accgttgcga cgcgccagat caattgcgcg
    27361 attaaattgc acacggatgt ccgcttcgtt ctgcgaatcg tcgaggaaca ccttccgttt
    27421 aataaccttc acgccagtac cttgcgcggc gcgcatcgcc tgggtattac cgatggttac
    27481 gctgtcgagg aagtaaagat tgtaacgctc cagtgcctgc atcactttct gcataccaaa
    27541 caggttagag gtcatcttgc tacccatgtg gttgttgatc cctgcggcat agggcacgtt
    27601 attgaccgca ctacgaataa tgcgctcaat ttcgtcgctg ctcatctccg ggcgcagcgt
    27661 atttttctcc agcggctgtt tactcagtgg tgccatcggg agatgaatca acacttcgtg
    27721 cccgctgtta tgcgctttgg tcgccatctc tctggcgtgc ggtgaatcgg gtaatacagc
    27781 gacggagata gcggagggca tcgccagcac ctggttttcg ttgtgcgggc gatacccaaa
    27841 atcatcaatg acgatggcaa gtttgccagc aagtacgggg gaggagagcg ccaacagagc
    27901 ggcaaatgca agaacgttac gacgaaatgg aaacaaaact tatcttccca accacggctg
    27961 tggattgacc gcctgaccct ggcggcgaat ttcgaaatag agtgaaggcc gaccctgacc
    28021 gccactgctg cccaccagtg caattggctg gcccgcgcga acctgcgaac caacgctcac
    28081 cagtgcgctc tgattatagc cgtaaagact catgtcgcct ttaccgtgct caaccaccac
    28141 caccagaccg taaccttgca gccagtcagc cagaatcact cgaccgtcgg caattgcttt
    28201 aacttcagta ccttcagagg caccgataac catccctttc cagcgtaatt caccctgtag
    28261 ctgctcgcca tagcgatgca gcgtcggccc gcgaaccggc cagaatgcct gaccacgcgg
    28321 cgcacccaga ccaccagttc gggacatcag cgatttttcg ctttcggtcg gtttgtaggt
    28381 ggtgcctttg cgcgtcgctt ctttctggcg gtcgcgaacc gcctgggcct cacgcgcttc
    28441 tcgttcagca cgcgctttcg ccgcggcttc cgcacgggca atgctgttac gcagacggga
    28501 ttcgttggca cgcagttcac ttagctgctg ctgaccttgc tggatggaag attccagccc
    28561 cgccagcgtc tttttacgct cgttcagagc ctgagtcagt ttcgcctgtt gggcgcgctg
    28621 ttcatataac agcgtttgtt gctcgctttg tttctcttcc agttcagcac gctgcatggc
    28681 gacttcttca cgcgtttgct tcaactgggc aatggtttct tgtcgcgcct ggttgagata
    28741 gccgaaataa gcctgtaaac gctgtccaca ctggctttct tcaccgctga gaattagctg
    28801 aataccggta tgctcgccct gacggaatgc ggcatccagt tgtgcggcga ggctgcgctc
    28861 ctgggcggct ttttgctgct ccagtttggc aatcgacgcg ttcatctcat caatctgttt
    28921 attgagttga ttgagcgtgt tttgcgtttc gcgcagctta cgggtggctt cagagatcgc
    28981 ttcttcctgc tttttcaatt gtgcgagcag gcttgcgcgt tgttgttgct tttggcgtac
    29041 cgcgcgctct tttgcggcga tatcggcctg aatagatttg agttggtcac gcacatccgc
    29101 gtgggcggaa aaggcgcaca acaatacgcc agcgctaaga acgctggcgt agatgatggg
    29161 cctgattgca aacctgcgcg gtttcacggc ccgtgtcatg gtattaatcg cctttcccct
    29221 catggggagg gattattcca cgatgaacag cggcttacca gtcatctctt gcgggatttc
    29281 catacccatc agcgacaaca tggtcggcgc gatgtcagaa agtttgccgc ctgcaaccgc
    29341 tttcacgttc ttatcaccaa cgtaaatcag cggaactggc aggttggtgt gtgccgtgtg
    29401 tgcctgaccc gttgccggat cgcgcatctg ttcagcgtta ccgtggtcag cggtgatcag
    29461 cagttgtcca cccacggact caaccgcttt cgcgacttct tccacgcagt gatccagtgc
    29521 ttcaaccgct ttaaccgccg cttccatcac cccggtgtga cctaccatgt cgccgttcgg
    29581 atagttacag ataatggtgt cgtatttgcc gctcttgatg gccgcaacca gtttttcggt
    29641 cagctctgcg gagctcattt ccggttgcag atcgtaggta gccactttcg gtgagttgat
    29701 cagaatgcga tcttcgcctt tgaatgactc ttctacgcca ccgttgaaga agaaagtaac
    29761 gtgggcatat ttttcggttt cggaaatacg caactgagtt ttgtcgtttt tcgccatcca
    29821 ctcgccgaag gtgttaacca gggaagcggg tgggtaagca accgcagttt tgatgtcagc
    29881 ggcgtattcg gtcagcatca cgaaatcgac gttaaccact ttcttacgcg cgaagccgtc
    29941 gaaatctgcg tttacgaatg cacgagtgat ttcgcgcgcg cggtcagcac ggaagttcat
    30001 gaaaatcagc gcatcgccgt cttccattgc cgcatctggc tgaccttctg cacgaataac
    30061 ggtcgctttc acgaactcgt cgttttcgtc gcgagcataa gctgcctgca aaccagcaac
    30121 ggcagtatcg gcctggaact caccctgcgc cagagtcagc aggtcatagg ctttttcaac
    30181 gcgatcccaa cggttatcgc ggtccatcgc gtagtaacga ccaatgatgg acgctacgcg
    30241 gcctttaccc agcgcagcaa atttctcttc aaattttttc agcgaggatt cagcactgcg
    30301 cggcggggtg tcgcgaccgt caaggaatgc gtgcaggtag attttttctg cgccgcgttc
    30361 agctgccagt tctaccatcg ccatgatgtg atcttcgtgg ctgtgtacgc cgcctgcgga
    30421 gagcagaccc ataatgtgta ctgctttacc tgcgtttttc gctttatcta ccgcgccagt
    30481 cagcactgga ttagcaaaga aagcgcgatc tttgatttca acgtccagac gagtcaggtc
    30541 ctgatacacg atgcggccgg cacccaggtt aacgtggcct acttcggagt tacccatctg
    30601 acggtcaggc agaccgactt ccagaccgga agcgtcgatc agggtatgcg gacgattggc
    30661 ccacagtgca tccattaccg gggttttagc actaaaaatg gcgttatcct gctgttcttc
    30721 acgatagcca tagccatcca gaatcaccag taccataggt tttttagaaa ccgacattgc
    30781 gacaacctca tactcaagag tcaaaatttg cgtaatttta ctacagctga atcgataaaa
    30841 tagcctctga agatcaaaat atacgaccgc tcgcggcaag ccgatgccag atttgcgctt
    30901 ttttttcgtc gtgccccgca aaattgcatt ccagttaacg cgctggctgt atttgccgca
    30961 ccgcgcaggt atactccttt cctggttttt ttaatcacta cgtcgggagt tgttaccccc
    31021 catgcaagaa attatgcaat ttgttggccg tcatcccata ctgagtatcg cctggatcgc
    31081 gttactggtg gcggttcttg tgactacgtt taagagcctg acctcgaaag tgaaggtgat
    31141 tactcgtggt gaagctacgc gtctgatcaa caaagaagac gctgtggttg tggatttacg
    31201 tcagcgtgat gacttccgta aaggccatat cgcaggttct atcaatctgt tgccgagcga
    31261 aatcaaagcc aacaatgttg gtgagcttga gaagcacaaa gacaaaccgg ttatcgtggt
    31321 agacggttct ggcatgcagt gccaggagcc tgcaaacgcg ctgacgaaag ctggttttgc
    31381 gcaagtattc gtgctgaaag agggcgttgc tggctgggct ggcgaaaact tgcctctggt
    31441 gcgcggcaaa taatttacct gtcgtctttc atgatgcagg ccgttagctg caattaggcc
    31501 cccagcgact tattgttata agcttctggg gacactccat tttgcagccg ccctgcaaca
    31561 tgaaatttca gggtaaaaaa acaggagcta aattatggcc aatgttgaaa tctataccaa
    31621 agaaacctgc ccgtattgcc atcgtgcaaa agcactgctg agcagcaagg gcgtgagttt
    31681 tcaggagttg ccgatcgatg gcaatgccgc caagcgtgaa gagatgatca aacgcagcgg
    31741 tcgcaccacg gttccccaga tttttattga cgcacagcac attggcggct gtgatgactt
    31801 gtatgcattg gatgcacgtg gtggactgga tcccctgctg aaataacgtg tgaacgttgg
    31861 cattacattg cgcagtattt aaggacaaca cttaagggtt ttctacacat gtcagaacaa
    31921 aacaacactg aaatgacttt ccagatccaa cgtatttata ccaaggatat ctctttcgaa
    31981 gcgccgaacg cgccgcacgt tttccagaaa gattggcaac cagaagttaa acttgatctg
    32041 gatacggcat cttcccaact ggcagatgac gtatacgaag tggtactgcg tgttaccgta
    32101 acggccaccc tgggcgaaga aaccgcgttc ctgtgtgaag ttcagcaggg cggtattttc
    32161 tccatcgcgg gtatcgaagg cacccagatg gcacattgcc tgggcgcata ctgcccgaac
    32221 attctgttcc cgtatgctcg tgagtgcatc accagcatgg tatcccgcgg tacattcccg
    32281 caactgaacc ttgcgccggt taacttcgat gcgctgttca tgaactattt gcagcagcag
    32341 gctggcgaag gtactgaaga acatcaggat gcctgatgaa ccaacgtaat gcttcaatga
    32401 ctgtgatcgg tgccggctcg tacggcaccg ctcttgccat taccctggca agaaatggcc
    32461 acgaggttgt cctctggggc catgaccctg aacatatcgc aacgcttgaa cgcgaccgct
    32521 gtaacgccgc gtttctcccc gatgtgcctt ttcccgatac gctccatctt gaaagcgatc
    32581 tcgccactgc gctggctgcc agccgtaata ttctcgtcgt cgtacccagc catgtctttg
    32641 gtgaagtgct gcgccagatt aaaccgctga tgcgtcctga tgcgcgtctg gtgtgggcga
    32701 ccaaagggct ggaagcggaa accggacgtc tgttacagga cgtggcgcgt gaggccttag
    32761 gcgatcaaat tccgctggcg gttatctctg gcccaacgtt tgcgaaagaa ctggcggcag
    32821 gtttaccgac ggctatttcg ttggcctcta ccgaccagac ctttgccgat gatctccaac
    32881 aattgctgca ctgtggcaaa agtttccgcg tttacagcaa cccggatttc atcggtgtgc
    32941 agcttggcgg tgcggtgaaa aacgtcattg ccattggcgc ggggatgtcc gacggtatcg
    33001 gttttggtgc gaatgcgcgt acggcactga tcacccgtgg gctggctgaa atgtcgcgtc
    33061 ttggcgcggc gctgggtgcc gagcctgcca cctttatggg catggcgggg ctgggcgatc
    33121 ttgtgcttac ctgtaccgac aaccagtcgc gtaaccgccg ttttggcatg atgctcggtc
    33181 agggcatgga tgtacaaagc gcgcaggaga agattggtca ggtggtggaa ggctaccgca
    33241 atacgaaaga agtccgcgaa ctggcgcatc gcttcggcgt agaaatgcca ataaccgagg
    33301 aaatttatca agtattatat tgcggaaaaa acgcgcgcga ggcagcattg acgttattag
    33361 gtcgtgcacg caaggacgag cgcagcagcc actaacccca gggaaccttt gttaccgcta
    33421 tgacccggcc cgcgcagaac gggccggtca ttatctcatc gtgtggagta agtaatgtcg
    33481 tgtgaagaac tggaaattgt ctggaacaat attaaagccg aagccagaac gctggcggac
    33541 tgtgagccaa tgctggccag tttttaccac gcgacgctac tcaagcacga aaaccttggt
    33601 agtgcactga gctacatgct ggcgaacaag ctgtcatcgc caattatgcc tgctattgct
    33661 atccgtgaag tggtggaaga agcctacgcc gctgacccgg aaatgatcgc ctctgcggcc
    33721 tgtgatattc aggcggtgcg tacccgcgac ccggcagtcg ataaatactc aaccccgttg
    33781 ttatacctga agggttttca tgccttgcag gcctatcgca tcggtcactg gttgtggaat
    33841 caggggcgtc gcgcattggc aatctttctg caaaaccagg tttctgtgac gttccaggtc
    33901 gatattcacc cggcagcaaa aattggccgt ggcattatgc tcgaccacgc cacgggcatc
    33961 gttgtgggtg aaacggcggt aattgaaaac gacgtatcga ttctgcaatc tgtgacgctt
    34021 ggcggtacgg gtaaatctgg tggtgaccgt cacccgaaaa ttcgtgaagg tgtgatgatt
    34081 ggcgcgggcg cgaaaatcct cggcaatatt gaagttggtc gcggcgcgaa gattggcgca
    34141 ggttccgtgg tgctgcaacc ggtgccgccg catacaaccg ccgctggcgt tccggctcgt
    34201 attgtcggta aaccagacag cgataagcca tcaatggata tggaccagca tttcaacggt
    34261 attaaccata catttgagta tggggatggg atctaatgtc ctgtgatcgt gccggatgcg
    34321 atgtaatcat ctatccggca tacagtaact aatctctcaa caacgctccc ggatatccca
    34381 gctgccgcca ggcttcatac accactaccg acaccgcatt ggacagattc atgctgcggc
    34441 tgtccggcac catcggaatg cgaatttttt gttcagcggg cagggcatca agaatgctcg
    34501 ctggcaggcc gcgtgtttcc gggccgaaca tcagataatc gccatcctga tagcttacgg
    34561 cgctgtgagc aggcgtacct ttcgtggtga gggcaaacag gcgctgggga ttttctgctt
    34621 cgaggaacgc gcgatagtca tgatgacgcg taacggcggt aaactcgtga tagtccagcc
    34681 ccgcgcggcg caggcgctta tcgtcccagg caaatcccat cggttcgatg atatgcagac
    34741 gaaagccggt attagcgcaa agacggatga tgttgccagt atttggcgga atttctggtt
    34801 cgtaaagtac gatgtttagc atactgcccc cttagtgcgg gggcaggata gcagaaaatt
    34861 ggtgaaccgt gcggcctttt cccctcaccc cggccctccc cctgtctctt gatcaggaga
    34921 tctgatcaag agatagctcc ctgagggaga gggtt
//
LOCUS       DAJTNA010000019        34519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTNA010000019 DAJTNA010000000
VERSION     DAJTNA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34519
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.019"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(29..1753)
                     /locus_tag="OO161_000814"
     CDS             complement(29..1753)
                     /locus_tag="OO161_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5461981.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIVWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            2132..3754
                     /gene="pckA"
                     /locus_tag="OO161_000815"
     CDS             2132..3754
                     /gene="pckA"
                     /locus_tag="OO161_000815"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5461982.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(3830..5182)
                     /gene="envZ"
                     /locus_tag="OO161_000816"
     CDS             complement(3830..5182)
                     /gene="envZ"
                     /locus_tag="OO161_000816"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5461983.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGSSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5179..5898)
                     /gene="ompR"
                     /locus_tag="OO161_000817"
     CDS             complement(5179..5898)
                     /gene="ompR"
                     /locus_tag="OO161_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5461984.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            6126..6602
                     /gene="greB"
                     /locus_tag="OO161_000818"
     CDS             6126..6602
                     /gene="greB"
                     /locus_tag="OO161_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5461985.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            6699..9020
                     /locus_tag="OO161_000819"
     CDS             6699..9020
                     /locus_tag="OO161_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5461986.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSSKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLCLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDISQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILTATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            9458..9685
                     /gene="feoA"
                     /locus_tag="OO161_000820"
     CDS             9458..9685
                     /gene="feoA"
                     /locus_tag="OO161_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5461987.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            9702..12023
                     /gene="feoB"
                     /locus_tag="OO161_000821"
     CDS             9702..12023
                     /gene="feoB"
                     /locus_tag="OO161_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5461988.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGAHQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            12023..12259
                     /gene="feoC"
                     /locus_tag="OO161_000822"
     CDS             12023..12259
                     /gene="feoC"
                     /locus_tag="OO161_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5461989.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            12462..13340
                     /gene="rpnA"
                     /locus_tag="OO161_000823"
     CDS             12462..13340
                     /gene="rpnA"
                     /locus_tag="OO161_000823"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5461990.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTPNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(13369..14139)
                     /gene="bioH"
                     /locus_tag="OO161_000824"
     CDS             complement(13369..14139)
                     /gene="bioH"
                     /locus_tag="OO161_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5461991.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            14177..14860
                     /gene="gntX"
                     /locus_tag="OO161_000825"
                     /pseudo
     CDS             14177..14860
                     /gene="gntX"
                     /locus_tag="OO161_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
     gene            14919..15494
                     /gene="nfuA"
                     /locus_tag="OO161_000826"
     CDS             14919..15494
                     /gene="nfuA"
                     /locus_tag="OO161_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5461992.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            15855..17171
                     /gene="gntT"
                     /locus_tag="OO161_000827"
     CDS             15855..17171
                     /gene="gntT"
                     /locus_tag="OO161_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5461993.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQMLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(17216..19300)
                     /gene="malQ"
                     /locus_tag="OO161_000828"
     CDS             complement(17216..19300)
                     /gene="malQ"
                     /locus_tag="OO161_000828"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5461994.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(19310..21703)
                     /gene="malP"
                     /locus_tag="OO161_000829"
     CDS             complement(19310..21703)
                     /gene="malP"
                     /locus_tag="OO161_000829"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5461995.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLIGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            22315..25020
                     /gene="malT"
                     /locus_tag="OO161_000830"
     CDS             22315..25020
                     /gene="malT"
                     /locus_tag="OO161_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR5461996.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIATVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQTTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(25063..26079)
                     /gene="rtcA"
                     /locus_tag="OO161_000831"
     CDS             complement(25063..26079)
                     /gene="rtcA"
                     /locus_tag="OO161_000831"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5461997.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLANIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(26083..27309)
                     /gene="rtcB"
                     /locus_tag="OO161_000832"
     CDS             complement(26083..27309)
                     /gene="rtcB"
                     /locus_tag="OO161_000832"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5461998.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            27498..29096
                     /gene="rtcR"
                     /locus_tag="OO161_000833"
     CDS             27498..29096
                     /gene="rtcR"
                     /locus_tag="OO161_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5461999.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(29078..29836)
                     /locus_tag="OO161_000834"
     CDS             complement(29078..29836)
                     /locus_tag="OO161_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5462000.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(29853..30683)
                     /gene="glpG"
                     /locus_tag="OO161_000835"
     CDS             complement(29853..30683)
                     /gene="glpG"
                     /locus_tag="OO161_000835"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5462001.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(30728..31054)
                     /gene="glpE"
                     /locus_tag="OO161_000836"
     CDS             complement(30728..31054)
                     /gene="glpE"
                     /locus_tag="OO161_000836"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5462002.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            31244..32749
                     /gene="glpD"
                     /locus_tag="OO161_000837"
     CDS             31244..32749
                     /gene="glpD"
                     /locus_tag="OO161_000837"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5462003.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDTRLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(32801..33406)
                     /locus_tag="OO161_000838"
     CDS             complement(32801..33406)
                     /locus_tag="OO161_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462004.1"
                     /translation="MIRFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(33409..34176)
                     /locus_tag="OO161_000839"
     CDS             complement(33409..34176)
                     /locus_tag="OO161_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462005.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLAGLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(34179..>34519)
                     /locus_tag="OO161_000840"
     CDS             complement(34179..>34519)
                     /locus_tag="OO161_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462006.1"
                     /translation="AMLNPDERVIIAKRPLKKCDSWIEDVYIFQVVKDAWKFHNNNSL
                     RGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNAANARFVYQ
                     PFTRDQRARG"
BASE COUNT         8342 a         9368 c         8693 g         8116 t
ORIGIN      
        1 tacagcgtcg tatccgataa aagcgttgtc acttacttaa gtttatttcc agcgtttcac
       61 catctaccgt cagatcgcgc cacgctttcg gcctcgtccc cagttttcca tccttcgcag
      121 ctgtgagtaa tttttctttc gttatttcgt tcggaagtaa actcatatca agcgcgtccc
      181 attcttttct gtctgagtca aagccataga caatgactct gtcatcatta aaagcaaaca
      241 ggatccgctc cgcctgacca tcactattca gatcttgctc gaccaggacg caggcgtctt
      301 tttcaataca ggtcatcacg ttatagcgat cctggattaa ggccgaccag aatgtcgcat
      361 ccggtttaac agaaccaggg gcaattaaca cattttcggc taatactttt tccgatacct
      421 gttcctgaag atgttgctct ccatctaacg ccatcagcag atcccgcgcg cgtttcgggt
      481 ctttcataaa cccggcatcg cttttcagcg actcaagcgc cgcacgtcca tagcgaccgc
      541 tctgttcgag catgtagata gttacctggt ctggcgtgtt tttgccgctc tgataacgcg
      601 ccatatgact gttcacgcta atacgcatac tgtccagcac cggcgaatta agaagcacca
      661 gtatcaccaa caccaataac gaaaccgcaa ggttcacttt gccctgaaga acaaggggat
      721 tttgcccatt acgccagacg atactgacaa aataccccag cgaccacacc agtaacacca
      781 gcaccgccag cgcgccctgc aagcggtcga cagtccagcc gtactgagcg acccgcagcc
      841 ataacgccca ggcggcgacg aacacataca gcggagcaac cagcaaagcg gttttaatca
      901 ggcaacgcaa cggccccgtc cagggaagtg acgctttttg cggatcgcgg acaatagcca
      961 ttaagatcaa ttgcaaaaag gccagcgtta acagcaaccc ggcggcggag atgtggcgag
     1021 aaatcgcgct caggcccgta aacggcagag tgatgataaa catcagggtt agcaatgata
     1081 ctaacggcag caaccccgtg gcgattaacg tgaacaactt ttgaatagag tcgattaaac
     1141 gtgactgtgt gcgcgcgaga atcaccgcca gtgcggtaac cagacctaat gtaagataca
     1201 taaaccagtc ggttgcaaaa aagagcgtat tgaaaaactt gatgccaaca agtttaaaca
     1261 actcacccca cagtaaaagc accagccacg tcaggccatt ggcgagaaaa atcaccagta
     1321 atataagtac gttatgccat actgactggt aaaagtagct atagcggcta ctgccattgc
     1381 ggatacgaag gctttgttgt atccacggta acaacaacat tgccatcaac agcagatagc
     1441 agccaaaatc ccaaatcgcc ttttcagctc tccaggggtt catgccatca gtttgccact
     1501 tcagccaacc gctcatccct gtcgtggcaa taaacaccag cgccagccat ccccaaaggc
     1561 gcttctgttt aaaagagatc acggagaaga gtaaaacgga tgaaaacgca acggtcgcag
     1621 gcacgccata aacaatccaa ctgtgatttt ttccggcaag ccagacgatc aacaggtagc
     1681 aaaccagccc ctgaagtaat ccggtagcaa tcatccccca gcgtgttgcc ggtgaaagtt
     1741 caacgttatc catgttggtt atccagaatc aaaaggtggg ttaattatcg catccgggca
     1801 gtagtatttt gcttttttca gaaaataatc aaaaaaagtt agcgtggtga atcgatactt
     1861 taccgattga atttgcatca atttcattaa ggaatgcgat tccactcaca atattcccgc
     1921 catataaacc aagatttaac cttttgagaa cattttccac acctaaaatg ctatttctgc
     1981 gataatagca accgtttcgt gacaggaatc acggagtttt ttgtcaaata tgaatttctc
     2041 cagatacgta aatctatgag ccttgtcgcg gttaacaccc ccaaaaagac tttactattc
     2101 aggcaataca tattggctaa ggagcagtga aatgcgcgtt aacaatggtt tgaccccgca
     2161 agaactcgag gcttatggta tcagtgacgt acatgatatc gtttacaacc caagctacga
     2221 cctgctgtat caggaagagc tcgatccgag cctgacaggt tatgagcgcg gggtgttaac
     2281 taatctgggt gccgttgccg tcgataccgg gatcttcacc ggtcgttcac caaaagataa
     2341 gtatatcgtc cgtgacgata ccactcgcga tactttctgg tgggcagaca aaggcaaagg
     2401 taagaacgac aacaaacctc tctctccgga aacctggcag catctgaaag gcctggtgac
     2461 caggcagctt tccggcaaac gtctgttcgt tgtcgacgct ttctgtggtg cgaacccgga
     2521 tactcgtctt tccgtccgtt tcatcaccga agtggcctgg caggcgcatt ttgtcaaaaa
     2581 catgtttatt cgcccgagcg atgaagagct ggcaggtttc aaaccagact ttatcgttat
     2641 gaacggcgcg aagtgcacta acccgcagtg gaaagaacag ggtctgaact ccgaaaactt
     2701 cgtggcgttt aacctgaccg agcgcatgca gctgattggc ggcacctggt acggcggcga
     2761 aatgaagaaa gggatgttct cgatgatgaa ctacctgctg ccgctgaaag gtatcgcttc
     2821 tatgcactgc tccgccaacg ttggcgagaa aggcgatgtt gcggtgttct tcggcctttc
     2881 cggcaccggt aaaaccaccc tttccaccga cccgaaacgc cgcctgattg gcgatgacga
     2941 acacggctgg gacgatgacg gcgtgtttaa tttcgaaggc ggctgctacg caaaaactat
     3001 caagctgtcg aaagaagcgg aacctgaaat ctacaacgct atccgtcgtg atgcgctgct
     3061 ggaaaacgtc accgtgcgtg aagatggcac tatcgacttt gatgatggtt caaaaaccga
     3121 gaacacccgc gtttcttatc caatctatca catcgataac atcgttaagc cggtttccaa
     3181 agcgggccac gcgactaagg ttatcttcct gactgctgat gctttcggcg tgttgccgcc
     3241 ggtttctcgc ctgactgccg atcaaaccca gtatcacttc ctctctggct tcaccgccaa
     3301 actggccggt actgagcgtg gcatcaccga accgacgcca accttctccg cttgcttcgg
     3361 cgcggcattc ctgtcgctgc acccgactca gtacgcagaa gtgctggtga aacgtatgca
     3421 ggcggcgggc gcgcaggctt atctggttaa cactggctgg aacggcacag gcaaacgtat
     3481 ctcgattaaa gatacccgcg ccattatcga cgccatcctc aacggttcgc tagataatgc
     3541 agaaaccttc actctgccga tgtttaacct ggcgatccca accgaactgc cgggcgtaga
     3601 cacgaagatt ctcgatccgc gtaacaccta cgcttctccg gaacagtggc aggaaaaagc
     3661 cgaaaccctg gcgaaactgt ttatcgacaa cttcgataaa tacaccgaca cccctgcggg
     3721 tgccgcgctg gtagcggctg gtccgaaact gtaatgattt gaagctggag aatatctatc
     3781 cagtatctta tagaaagcaa aacgggaggc accttcgcct cccgtttatt tacccttctt
     3841 ttgtcgtgcc ctgcgcccgc gttaccggca ctggcagcca ggcgcgaatg gaaagcccgc
     3901 cccgctcgct gctgccaagc tccagcatcc cgttatggtt atccacgata cgctgcacaa
     3961 ttgccagccc taatcccgtg ccgctaatgg tgcgtgcact gtcgccgcgg acaaacggct
     4021 ggaacaggtg cttacgttgt tccggcgcaa ttcccggacc gtcatcttcc acctggaacc
     4081 aggcgcgatt cggctccgtc ccgctgctga ctttgatcca gccattgccg taacgggcgg
     4141 cgttgaccac catattcgcc accgcgcgtt tgatcgacag cgggtgcatt ttcacttcaa
     4201 tgctgccggg gtaaagcgcg gtttcaattt cccgctcata gccactttcg gcagcaataa
     4261 cctcaccgag tactgcgttg agatccgcca tttccatcgg catctcctgc ccggtgcgca
     4321 ggtagtcgat aaactgctca atgatggcgt tgcactcttc gatatcttta ttgatcgatt
     4381 cggccaggta gccatcctgt tcgctcatca tctcggtcgc caggcgaata cgcgtcagcg
     4441 gcgtgcgtaa gtcatgactt acccccgcca tcagcagcgt gcggtcatcc gccagttgct
     4501 taacaccagc cgccatatgg ttaaaggcgc gggtaaccga acgtacctcg gacgcgccat
     4561 attcacgcag cggcggtgga ataatccctt taccgacctg caaagcagca tgttcgagat
     4621 cgaccaacgg tcggttctgg atacgaataa acagccacgc cccgcctatc gccaatagca
     4681 taatcgccag cgtatagcgg aacagcggag agaaatcgcc ctgatgaatt tcggtcagcg
     4741 gcacgcgtac ccagatattg ggcgacagcc aggttttcag ccagacgaca ggcgaacttt
     4801 tgttgacctc aacgcgcact tccgtcgggc cgcccagttg ctgtgccatc tgatggctta
     4861 agaattcata gtgttgcgcc caacgcagac ctgcctcttc ggcagcctcg ttggagtaga
     4921 gagagatccc cagctcacgg tagatctccc gacggaaagc gggaggcaca accaactgcg
     4981 tgccgtcctc cagttgcagt ttgtcggtca tcaacatacg cacttcgtac gcgaggactt
     5041 tattaaactg ctggaggctc ggcaaaatcg cgaagttcag caccaccaga taagtcgtca
     5101 ccaggctggc gaacagcaag gtgacgatga gcaataacgt acgggcaaat gaacttcgtg
     5161 gcgagaagcg caatcgcctc atgctttaga gccgtccggt acaaagacgt agcccaggcc
     5221 ccaaacggtc tgaatgtaac gcggatgcgc tggatcttct tctaccatgc ggcgcagacg
     5281 cgaaatctgc acgtcgatgg agcgttccat tgcggaatat tcacgaccac gggcaaggtt
     5341 catcagctta tcgcgggaga gcggctcacg cggatggctg accagtgcct tcagtaccgc
     5401 aaactcaccg ctggtgagcg gcatcggctc atcttcgcgg aacatttcgc gcgtaccgag
     5461 gttaagtttg aacttaccga aagcaattac cgcctcttcc tgtgacggtg cgcctggcag
     5521 ttcgttcgcc tgacgacgca gcaccgcacg aatacgggcc agcagttcac gcgggttaaa
     5581 cggttttgga atgtagtcgt cagcgccaat ctccaggcct acgatacggt ccacttcttc
     5641 ccctttcgcc gtcaccataa tgatcggcat cgggttgctc tgactacgaa gacgtcggca
     5701 aatcgacaag ccatcttcac caggtaacat taaatccagt accataagat ggaaagattc
     5761 acgagtcagc aggcgatcca tctgttctgc attagcgacg cttcgaacct ggaagccttg
     5821 ttcggtgaga taacgttcca gcagcgcacg caggcgcatg tcgtcatcga ccaccagaat
     5881 cttgtagttc tcttgcattg tttgtactcc caaaggttcg caacaatttg taagcgtgta
     5941 ttcttaaaaa agctcacgtt cgtcaccagc taaatctggt atgaatttca gcctaaattg
     6001 ttacaaagca tattaaacag cagcttaagt atacaattta ttcggcgaaa cattattgat
     6061 tctgttgata tgatcacgtt atacccaatg tgcgcattat caaacagaca aagggaatca
     6121 acgagatgaa aacgcccctg gttacccggg aagggtatga aaaactcaaa caagagctta
     6181 attatctctg gcgtgaagaa cgcccggagg tcacaaaaaa ggtgacctgg gccgcaagtc
     6241 tgggcgaccg cagcgaaaat gctgactatc agtataataa aaagcgtctg cgtgaaatcg
     6301 accgtcgcgt gcgctatctc actaaatgca tggaaaatct caaaatcgtc gattactccc
     6361 ctcagcagga aggcaaagtc ttttttggcg cgtgggtgga gattgaaaac gacgatggcg
     6421 tgactcaccg tttccgtatt gtcggctacg atgaaatttt tggccgtaaa gattacatct
     6481 ctatcgattc cccgatggcc cgcgcattgc tgaaaaaaga agtcggcgat ctggcggtgg
     6541 tgaatacccc tgccggggaa gcgagctggt atgttaatgc tatcgagtac gtgaaaccgt
     6601 aaggaagagt cttaacctcc tgccgatggc tggcattttt gccagccagt ccgtataact
     6661 atcccctgat ttttgatccg aaaagatgaa ctcaaaccat gatgaatgat tcgttctgcc
     6721 gcattattgc gggtgaaatt caggcgcgcc cggaacaggt tgacgctgcc gttcgcctgc
     6781 ttgacgaagg gaataccgtg ccgtttatcg cacgttatcg taaggaaatc accggcggtc
     6841 tggatgacac gcagctgcgt aatctggaaa cgcgtctgag ctatctgcgc gagctggaag
     6901 agagacgtca ggcgatcctc aagtccattt ccgagcaagg caaactcacc gatgatctgg
     6961 cgaaggccat caacgccacc ctaagcaaaa ccgaactcga agacctctac ctgccctaca
     7021 aacctaaacg ccgcacccgc gggcaaatcg ccattgaagc agggcttgag ccgttggctg
     7081 acctgctgtg gagcgatccg tcacacacgc cagaagtcgc cgctgcacaa tatgttgatg
     7141 ccgataaagg cgtggcagat accaaagccg cgctggatgg cgcgcgctat atcctgatgg
     7201 aacggtttgc cgaagatgcc gcgctgctgg cgaaagtgcg tgattatctg tggaagaacg
     7261 cgcatttggt ttctacggtg gtgagcagta aagaagagga aggggcgaaa ttccgcgact
     7321 atttcgatca tcacgaaccg ttgtccacgg tgccttctca ccgcgcgctg gcgatgttcc
     7381 gtggacgtaa cgaaggcgta ctccagcttt cgctgaatgc cgatccgcag ttcgatgagc
     7441 cgcccaaaga gagctattgc gagcaaatca tcatggatca cctttgcctg cgcctgaaca
     7501 atgccccggc ggatagctgg cgcaaaggcg tagtgagctg gacctggcgc atcaaggtgc
     7561 tgatgcatct ggaaaccgaa ctgatgggta ccgtgcgcga acgcgcggaa gatgaagcaa
     7621 tcaacgtctt tgcccgtaac ctgcacgatc tgctgatggc ggcccctgcc ggactgcgtg
     7681 caacgatggg cctcgatccg ggtctgcgta ctggggtaaa agtggcggtg gtcgatgcca
     7741 ctggcaaact ggtagcgacc gataccattt acccgcacac cggacaagcc gcaaaagcag
     7801 cgatgaccgt tgctgccttg tgtgaaaaac ataacgttga actggtggcg atcggcaacg
     7861 gtacagcttc ccgcgaaact gaacgtttct atctcgacgt gcagaagcag ttcccgaaag
     7921 tgaccgcaca gaaagtgatc gtcagcgaag ctggcgcgtc ggtttactca gcttccgagc
     7981 tggcagcgca ggagttcccg gatctcgacg tttcgctgcg tggcgcggtg tctatcgccc
     8041 gccgtttgca ggatccgctg gcggagctgg tgaaaatcga tccgaaatct atcggcgtag
     8101 gtcagtatca gcatgacatc agccagacgc aactggcccg caaactggac gcagtagtag
     8161 aagactgcgt aaacgccgtt ggcgtcgatc tcaacactgc ttctgttccg ctgttaaccc
     8221 gcgtggcggg cctgacgcgc atgatggcgc aaaacatcgt ggcctggcgc gatgagaacg
     8281 gccagttcca gaaccgtcag caactgctga aagtcagccg tctggggccg aaagccttcg
     8341 agcagtgcgc gggcttcttg cgcattaacc acggtgataa cccgctggat gcttctaccg
     8401 tccacccgga agcctatccg gtggtggaac gcattctgac agcaacacag caggcactga
     8461 aagatctgat gggtaacagc agcgaactgc gtaacctgaa agcgtctgac tttactgatg
     8521 aaaaattcgg tgtgccgaca gtaactgaca tcatcaaaga gctggaaaaa ccgggtcgcg
     8581 atccgcgtcc ggaatttaaa accgctcagt ttgccgatgg cgtcgagaca atgaacgacc
     8641 tgcaaccggg tatgatcctc gaaggtgcgg tgaccaacgt caccaacttt ggcgcgtttg
     8701 tcgatatcgg cgtgcatcag gacggcctgg ttcacatctc ttcattgtcg aacaagtttg
     8761 tggaagatcc gcataccgtg gtgaaagcgg gcgacattgt gaaggtgaaa gtgctggaag
     8821 tggatcttca gcgtaaacgt atcgccctga ctatgcgtct ggacgagcag cctggcgaaa
     8881 ccaacgcccg tcgcggcggc ggtaatgaac gcccgcaaaa caaccgcccg gcagccaaac
     8941 cacgcggtcg tgaagcgcag cctgccggta atagcgcgat gatggatgcg ctggcggcgg
     9001 caatgggcaa aaaacgttaa acgcccggac tggcccctaa acctgcgcga taaggcgttc
     9061 acgccgcatc tggcaacgaa cgccttgcct gacataaaag tgccggagaa tatctccggc
     9121 ctttttattc cacaaccaaa ctcataatat attccggcaa tatttatcac ttcattaaca
     9181 actgaaacct taattaaaca ttagccagtc cgggtaattc actattcgaa ttatattttc
     9241 gttgcgatat aaccttgagc cacatcaaca ttgagtcaga ttattattca aaccaacatt
     9301 cgcacacatt ttaagtattg ctgatagaaa ccattctcat tatcattgtg ttgttaatta
     9361 tttaatctct cctttgttgg caaatcatct ggtctcatgt cgctgtcaaa cgccccatga
     9421 ggtagttatc cagttaatga gaaacaagta ggcacctatg caatacactc cagatactgc
     9481 gtggaaaatc actggctttt cccgtgaaat cagcccggca tatcgccaaa aactgctttc
     9541 tcttggcatg ttacctggct cctcttttaa tgtggtgcgc gtcgctccac tcggcgaccc
     9601 cattcatatc gaaacccgtc gtgtgagcct ggtattacgc aaaaaagatc tggccttatt
     9661 agaagtggaa gcggtttcct gttaatacgg tgataacaac aatgaaaaaa ttaaccatag
     9721 gcttaattgg taatccaaat tctggcaaga caaccttatt taaccagctc actggcgcac
     9781 atcagcgtgt aggtaactgg gctggcgtta ccgtcgaacg taaagaaggg caattctcca
     9841 ccaccgatca tcaggtcacg ctggtggacc tacccggcac ctattctctg accaccatct
     9901 catcgcagac ctcgctcgat gagcaaatcg cctgtcacta cattttgagt ggcgacgccg
     9961 acctgctgat taacgtggtg gatgcgtcta accttgagcg taacctgtac ctgacgctac
    10021 aactgctgga actcggcatt ccctgcattg tggcactgaa catgctcgac attgccgaga
    10081 agcaaaatat tcgtattgaa attgatgctc tgtcggcgcg tctgggctgt ccggtgatcc
    10141 cgctggtttc aacccgtggt cgcggtattg aagcgctcaa gctggcgatt gatcgctata
    10201 aagctaacga gaatgtggaa ctggtgcatt acgcacagcc gctgctcaac gaagcagatt
    10261 cactggcaaa agtgatgcct tccgacatcc cgctgaaaca acgtcgctgg ctgggcctgc
    10321 aaatgctgga aggcgatatc tacagccgcg cctacgccgg tgaagcgtcg cagcatctgg
    10381 atgccgccct cgcccgtctg cgtaatgaga tggacgatcc ggcgctgcac attgccgatg
    10441 cgcgttacca gtgcattgct gccatctgtg atgtggtaag caacaccctg acggcagaac
    10501 ccagccgttt caccactgcg gtagataaaa tcgtgctcaa ccgtttcctc ggtctgccga
    10561 ttttcctctt tgtgatgtac ctgatgttcc tgctggctat caacatcggc ggggcgttac
    10621 agccgctgtt tgacgtcggc tccgtggcgc tatttgtgca tggtattcaa tggattggct
    10681 acacgctcca cttcccggac tggctgacta tcttcctcgc ccagggcctg ggtggcggca
    10741 ttaacaccgt gctgccactg gtgccgcaga ttggcatgat gtacctgttc ctctccttcc
    10801 ttgaggactc ggggtatatg gcgcgtgcgg cgtttgtgat ggaccgtctg atgcaggcgc
    10861 tgggcttgcc ggggaaatcc tttgtgccgc tgatcgtcgg tttcggttgt aacgtaccgt
    10921 cggtaatggg tgcacgtacg cttgatgcac cgcgtgaacg tctgatgacc atcatgatgg
    10981 caccgtttat gtcctgcggc gcgcgtctgg ctatcttcgc agtattcgcg gctgccttct
    11041 tcgggcagaa cggtgcgctg gcggtcttct cgctgtatat gctgggtatt gtgatggcgg
    11101 tgctgactgg cctgatgctc aagtacacca tcatgcgcgg tgaagcgacg ccgtttgtca
    11161 tggagttgcc ggtctatcat gtaccacacg ttaaaagcct gattatccag acctggcagc
    11221 gtctgaaagg cttcgttctg cgtgctggta aagtgatcat catcgtcagc attttcctga
    11281 gcgctttcaa cagcttctcg ctgagcggga aaatcgtcga taacatcaac gactcggcgc
    11341 tggcgtccgt cagccgggtg atcaccccgg tcttcaagcc aattggcgtg catgaagata
    11401 actggcaggc aacggttggc ctgtttacag gtgccatggc gaaagaagtg gtagtgggta
    11461 cgctcaacac cctctacacc gcagaaaata ttcaggacga agagttcaat ccggcagagt
    11521 ttaacctcgg tgaagagctg ttcagtgcga tagatgaaac ctggcagagc ctgaaagaca
    11581 ccttcagcct tagcgtactg atgaacccca ttgaagccag caaaggcgac ggcgaaatgg
    11641 gtaccggggc gatgggcgtg atggatcaga aattcggtag cgcagcagca gcttacagct
    11701 acctgatttt cgtcctgctg tatgtaccat gtatctcggt gatgggagcc atcgcccgtg
    11761 aatcaagccg tggctggatg ggcttctcca tcctgtgggg gctgaatatc gcttactcgc
    11821 tggcaacatt gttctatcag gtcgccagct acagtcagca cccaacttac agcctggtgt
    11881 gcattctggc ggttatcctg tttaacatcg tggttatcgg tctgctgcgc cgcgcgcgta
    11941 gccgggtgga tatcgaactg ctggcaaccc gcaagtcggt aagcagttgc tgcgcagcca
    12001 gcaccaccgg tgattgccat taatggcttc acttattcag gtgcgcgatt tgctggcgtt
    12061 acggggccgt atggaagcgg cccagataag ccagacattg aacactccac agccaatgat
    12121 taacgccatg ctgcaacaac tggaaagtat gggcaaagcc gtgcggattc aggaagaacc
    12181 tgacggctgc ctctctggca gttgtaaaag ctgcccggaa ggaaaagcct gtctgcgcga
    12241 gtggtgggcg cttcgttaac cttactccat cgcctgtttt ggaaagcccg gtatgcgtct
    12301 gcatccgggc ttttttgcgt gcggctttcc ataaaaatgc aactcttgca gcacggcgta
    12361 agttcctttg aaagcatctc gcagggatga aaactcgcta atacacaggt gtggagtggc
    12421 gcgtagagtc gcggcattca aacaacaggt gaaggaacgc catgagcaaa aagcagagtt
    12481 ccaccccaca cgatgcgctg ttcaaactct ttttacgcca accggacacg gctcgtgatt
    12541 ttcttgcgtt tcatttaccg gcacccattc acgcgctttg tgatatgaaa accctcaagc
    12601 tggagtcgag cagctttatt gatgacgatc tgcgtgaaag ctattccgat gtgctgtggt
    12661 cggtgaaaac ggaacaagga ccaggataca tctattgtct gattgaacat caaagcaccc
    12721 caaacaaact gatcgcattt cgcatgatgc gttacgctat tgccgcaatg caaaatcacc
    12781 ttgatgctgg atacaaaacg ttgccgatgg tggtgccatt gttgttttac cacggtattg
    12841 aaagccccta tccctattcg ctgtgttggc tggattgttt cgccgatccc aaactggcaa
    12901 ggcagcttta tgcctccgca tttccgctga ttgatgtcac cgtcatgcct gatgatgaaa
    12961 tcatgcagca ccgacgtatg gcgctgctgg agttaattca aaaacatatt cgtcaacgcg
    13021 acctgatggg gctagtagag caaatggcct gcttattaag tagtggatac gctaatgaca
    13081 gacaaatcaa agggctgttt aattacatac tgcaaaccgg cgacgcggta cgttttaacg
    13141 attttatcga cggcgttgcc gaacgttcac cgaaacacaa ggagagttta atgacaattg
    13201 cggaaagatt gcggcaggag ggggaacaat ccaaagccct gcatatagcc aaaataatgc
    13261 ttgaatccgg agtccctctt acagacatca tgcgctttac cggggtgtca gaagaagagt
    13321 tggctgcggc gagccagtaa atttctgtct cgccatttca aaagttgcct acaccctctg
    13381 cttcaacgcc accagcaggt gacaaaactc gaccggatgc gaaataaacg gcgcatgggc
    13441 cgctttggcg aagatatatg atttgctgcg aggccagagt ttatccagca tcggcaccac
    13501 tttgcgcggc accagaccat cgagatagcc atacaatcgc aaaaacggca tgggcacgtt
    13561 ttgcagcggc tgacggagat caaccgtttt caggatttcc agcccgccat taagcacgtc
    13621 aacctccggc atcggtaacg ccagaacggt tttcttcaac gcccgcgcat cctggcgcgc
    13681 cgtttcagtc cccatggttt gtaacgccag gaaccgctcc actgtacgct gaaaatcatc
    13741 actgagttgc tgctgaaatc ccgccagcac gtccggtttt atccccggcc aatcgtcacg
    13801 agcactaaaa caaggtgacg acgccacggt gaccagcgcc tgaacacgct cgggatgggt
    13861 taacgcaatc tggcttgcca ccagcccgcc cagactccag cctaaccaaa tggctttatc
    13921 aggtgcctgt tgcagcacgg cttcggccat atcagcaagt gacagcgcac caaatccctg
    13981 gctgcgcccg aagccgggca gatcaacaag gtgcagcgta aaatgcgagc taagttcctc
    14041 gtcaatgcaa cgccacactt cggcattcag tccccatccg tgcagcagca caagatgaac
    14101 attcccctga cctttggtct gccaccagat gttattcatc cgctattgtt ctcttttgac
    14161 ttacaaggat gaacatatgc taacagtacc gggattatgc tggctatgcc gaatgccgct
    14221 ggcgttaggt cattgaggga tttgttcggt ctgctcacgc gccgcccgta cagataaaac
    14281 gttatgccca caatgtggat tacccgccac acactcacat cttccctgcg gtcgctgcct
    14341 gcaaaaacca ccgccctggc aaagactggt cacggttgcc gactatgcgc cgccgttaag
    14401 tccgcttatc caccagctta aattttcccg gcgcagtgaa atcgccagcg ccctgtcacg
    14461 tctgttacta ttggaagtct tacacgctcg tcgcaccacc gggttgcaat tgccggatcg
    14521 catcatcagc gttccgttat ggcagcggcg tcactggcgt cggggattta atcagagcga
    14581 tttgctgtgt cagccgttat cacgctggtt gcactgccga tgggatagcg aagccgtcac
    14641 acgaacacgg gccactgcga cccagcattt tctcagtgcc cggctacgca agcgcaacct
    14701 gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc catatggtga ttgtggatga
    14761 tgtcgttacc accggaagta ccgtcgcaga gattgcgcag ttgcttttac gcaatggtgc
    14821 ggcgactgtc caggtctggt gcctttgtcg aaccttgtag agcctcgatg atgggcgtat
    14881 tataaccaac taaaatagtc aactattagg ccattactat gatccgtatt tccgatgctg
    14941 cacaagcgca ctttgccaaa ctgctggcaa accaggaaga agggacacaa atccgcgtat
    15001 ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt ttcttattgt ccgccggatg
    15061 ccgtggaagc caccgacact gccctgaaat ttgacctgct gaccgcgtat gttgatgagt
    15121 taagcgctcc ttacctggaa gatgcagaga tcgattttgt taccgaccag ttgggttctc
    15181 agctaacact gaaagcgcct aacgccaaaa tgcgtaaagt ggcagacgat gcaccactga
    15241 tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc acagcttgct ggtcacggtg
    15301 gccgcgtttc actgatggaa atcaccgaag acggttacgc cattctgcaa tttggcggcg
    15361 gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga agggatcgag aagcagctgc
    15421 taaacgaatt cccggagctg aaaggtgtgc gcgatctcac cgaacaccag cgcggcgaac
    15481 actcctacta ctaagttatc ccctcttttg tagattgccc gatgcgacgc taaagcgtca
    15541 tttcgggcct acgttgaaca tgtgccagac gttggcgcag cattgctttg gcaaatccct
    15601 cccttcgttt ctccgcgata atatgaccaa cctctcataa tttaaattta ctccgctctg
    15661 gtgattctca aacgccagat gttacccgta tcattcacat gggtaccaaa catactcctg
    15721 acatctgact acaataatta gttttagtgg gtatcagtcg tggtgccgca atatctctgt
    15781 tcccgattgg gataattaga gtttgtcgtc agaaaattga cgttacccat aacaaatgaa
    15841 aggccaggta aatcatgcca ttagtcattg ttgctatcgg tgtaatcttg ttgttgctcc
    15901 tgatgatccg tttcaaaatg aacggcttca tcgctctcgt cctcgtggcg cttgctgttg
    15961 gattaatgca agggatgccg ctggataaag ttattggctc catcaaagcc ggtgtcggcg
    16021 ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc gatgctgggc aaaatgctgg
    16081 cagactgcgg tggcgcacaa cgtatcgcca ccaccctgat tgccaaattt ggtaaaaaac
    16141 acatccagtg ggcggtggta ctgaccggtt ttaccgttgg ttttgccctg ttctatgaag
    16201 tgggctttgt gctgatgctg ccgctggtgt ttaccatcgc ggcttctgcg aatattccac
    16261 tgctgtatgt tggtgtacca atggcagctg cactgtctgt gacccacggc ttcctgccac
    16321 cgcatccggg tccgactgcg attgccacca ttttcaatgc cgacatgggt aaaaccctgc
    16381 tgtacggtac tattctggca atcccgaccg tgattctcgc cggtccggtt tacgctcgcg
    16441 tgttgaaagg tatcgataag ccgatccctg aaggtctgta cagcgcgaaa accttcagcg
    16501 aagaagagat gccgagcttt ggcgtcagcg tctggacctc tctggtgccg gtagtgctga
    16561 tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg tcatgctttc ctgccggtag
    16621 cggagttcct cggtgacccg gtaatggcta cgctgattgc cgtgctgatt gcgatgttca
    16681 cctttggtct gaaccgtggt cgttcaatgg atcagattaa cgacacgctg gtttcttcca
    16741 tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg cggtgcgttc aagcagatgt
    16801 tggtagacag cggcgtggac aaatacattg cttccatgat gcacgaaacc aacatttctc
    16861 cgctgctgat ggcctggtcg attgctgccg tactgcgtat cgcgctgggt tccgcaaccg
    16921 ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat tgcaacgacg ggtgttagcc
    16981 cggagctgat ggttattgcg gtgggttccg gtagtgtgat tttctctcat gtgaacgatc
    17041 cgggcttctg gctgttcaaa gagtacttta acctaactat cggcgagacc atcaaatcct
    17101 ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt aggctgtctg ctgctgaata
    17161 tggtgatttg ataacactga ctgccggatg catcgcatcc ggcatcaacc gcactctact
    17221 gcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt cagcaactta ttcacgccat
    17281 catcggcaaa catcgtctca agcgttgcgg aaagcttgcg tcgccagttt ttatactggt
    17341 aactggtgcc aggaatattc accggttcgg ccatatccag ccagtcttcc ggctgtagtc
    17401 ctaacagagc actgttactg tcggcaatgt agcgctgcaa accacgatta agcgtcggcg
    17461 tcatcgacat caacgatgcc ttatgcccgg cacgttttgg cagacaacca tatttatgca
    17521 gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg atacagaccg cgcagtacca
    17581 cttcatccgg atacagcccc agggttttgc ccaacgttag atccccgctc tcccagtaac
    17641 cgcgcagcgt aggcaggtca tgtgtcgccg caaccgccat cgactgctcc ggatacgctt
    17701 tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata gagcactttg taggagtaca
    17761 caccgctgct gcgcagctta ccgacaatct ctaccggtac ggtaccgaga tcttcaccaa
    17821 tcaccataca gcgatgacgt ttactttcga gtgccagaat cgagagcaga tcatccaccg
    17881 gatagtgaac atacgcgccc tgatctgccg tctcgccata cggtatccac cacaaacgca
    17941 gcatcgacat cacatggtca attcgtaatg cgccgcagtt ttgcatattg gcacgcaaca
    18001 gctcgataaa cggttcatag gcacgcgcgg tgatgatatg cgggtccatt ggcggtaatc
    18061 cccagttctg ccccaacggg ccgaggatat ccggcggcgc gccaaccgac gctttcaggc
    18121 aatatagttc acggtcacac caggtttccg ccccaccttc cgctacgcca accgccagat
    18181 cacgatacaa gccaatcggc atttcatagc cctggcttat ctcccagcag gcggcaaact
    18241 ggctgtaagc cagccactgc aaccagagat aaaaatcgac gtcatcacga tgttcttcgc
    18301 agaactgacg cacttctggt gaatccacgt tctgatacat ctctggccat gcaggccagc
    18361 cccagcgcat ttcgtcctct ttcacttgct gggcatgtag cgcatcaaag gctgcctgcc
    18421 agaacaggct gtcgccctgc tctgcaacaa actggcgaaa cgcggtcatc tgctcatcat
    18481 cacgttgcgc gaaacctttc cacgccattc gtaatgctgt catttttagg gcggtaaccg
    18541 tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag cgtctgttgc gtggtcggca
    18601 actgccacca ggcctgagcc tcttcgctaa gatggaaatc ttcaacggcg ttaacgtcga
    18661 tataaatcac attcagccaa cggcgagaag acgggctgta tgggctggca ctctccggat
    18721 ttgccggata gagcgcatga atcgggttca ggccgatgaa cgacccgcca cgttttgcca
    18781 catccaccag catcgctttg agatcgccaa aatccccaat accccagttt ttttccgatc
    18841 gcagcgtata aagctgaacg caggcacccc acagcttttg tttattcagc aacgcctgcg
    18901 gttcgtaaca gcgtttcggg gcgacaatca cccggcaatg cgcgcgctgg tcgtcctggg
    18961 tgagtgtcag cgtgtgataa ccttccggca gcttcgtcgg tagattgaac gctttgcccc
    19021 ccgttacatg gcctttgtac tgtgttcctt cttcggtggt cagcagccag ctatattcgc
    19081 cgctgccctc caccaccatc ggcatttttt tgccgctggt ataaaccatg acattcggga
    19141 ctggcgttac cgccactttc gtggcggtac gttgatgcat cgcgtcaagc aaacgccgtt
    19201 tggtttcggc gctaatcgac tgcggtttac cgtgggcatt gatgtaattg gggctaatcc
    19261 ccgccgccag cgcggcatta tccagacgtt tgctttccat cgagcttcct tagcgttttg
    19321 cctgccagat acgagcctga taatcgcgaa tagagcgatc cgagctaaac ataccgcagc
    19381 gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg gtcgcggtac agcacatcca
    19441 cctgcttttg tgcctcaaca taggctgcga aatccgccat caccagatac ggatcgccgc
    19501 cttgtttgcc gatactgtgc agcatctggt cgaaggcatg cttatcgccg tcgctgtatt
    19561 taccgctttc cagctctttc aatactgcgt ccagcacctt atctttcttc cgccatttca
    19621 ccgggtcgta gcctttggcc agaattgcct tcacttgttc cacggtatga ccaaaaataa
    19681 agatattttc ttcaccgact ttctcggcga tttcaacgtt cgcaccatcc agcgtaccga
    19741 cagtaagcgc accattgagc gccagtttca tattgccggt accggaagct tctttacctg
    19801 cagtcgaaat ttgttcggag atatccgccg ccgggatcag tttttccgcc gccgaaacgc
    19861 aataatccgg caggaacacc accttcaact tatcgccaat cagtggatcg ttgttgatca
    19921 cgtcagccac tttgttgatc gcaaagataa tattcttcgc caggtagtag cccggtgccg
    19981 ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc ctgcgggttt tcacgaattt
    20041 ctttgtacaa cgccagaata tgcagcagat tcaggtgctg gcgtttgtac tcatgcagac
    20101 gtttaatctg aatatcgaaa atcgcctgtg gattgatctc aataccggta cgaactttca
    20161 caaactccgc cagacggact ttattcgcct gcttgatctc gcgatattgc tgacggaatt
    20221 tcgcatcatc agcgaatttt tccagattga tcagctgatc gagatcgtta gcccactctt
    20281 tttgcagtaa tttatccaac agagccgcca gtgcagggtt gcactgtttg atccagcgac
    20341 gtggggtaat accgttggtg acgttatgga atttgttcgg ccatagctgg tgatattccg
    20401 ggaacagatc tttcaccacc agatccgagt gcagcgccgc aacaccgttc accgcgaaac
    20461 cgccaaccac acacaggttc gccatatgca cttgtttatc gtgcacaacc gccagtttgg
    20521 cccacacttt ttcatcgccc ggccaggttt tctccaccag cgttttaaag cgagtattaa
    20581 tttcgttaat aatctgcata tggcgcggca gtaagccttt caccagtttc acatcccagc
    20641 gttccagcgc ttctggcatc agggtatggt tggtgtaagc gaaagttttg ctggtaatgg
    20701 cccaggcgtc atcccagctc atctggtgct catcgatcag cacgcgcagc agttctggaa
    20761 tcgcgatagt tgggtgggta tcgttcagct gaataacttc ataatccgcc agttcgtgca
    20821 gtttacgccc cgccagatga tggcgacgca aaatatccgc taccgaacag gcacactgga
    20881 agtactgctg catcaagcgc agctttttac cggcagtatg gttgtcgttt ggatagagaa
    20941 ctttggtcag tttttccgca ttgatgccct gctgttcggc acgcaggaaa tcaccgtcgt
    21001 taaatttagt cagatcaaac ggatgcgcgt gcgtcgcctg ccacagacgc agcggctgcg
    21061 ccacgccatt acgatagccg acaacgggga gatcccacgc ttgaccggta atggtaaact
    21121 ccggctccca gcgtccgtct ttcgtcactt taccgccaat ccctacctgc acatccagtg
    21181 cttcgttgtg gcggaaccac gggtagttac tgcgatgcca gtcatccggc gcttcaacct
    21241 gtttgccatc gacaaaagac tggcggaaca aaccatattg atagttcaga ccgtaacccg
    21301 tcgcagactg accgatagtt gccattgagt cgaggaagca cgccgccaga cgtcccagac
    21361 caccgttacc cagcgccggg tcgatctctt cttccagcag gtccgtcaga ttgatgtcat
    21421 aagccttcaa cgaatcctgt acatcctgat accagccgag attcaacagg ttgttgcccg
    21481 tcaggcgacc aatcaaaaac tccattgaga tgtagttaac atgtcgctga ttcgccaccg
    21541 gcttggcgaa tggctgagca cgcagcattt cggccagtgc ttcactcact gctagccacc
    21601 actggcgagg agtcatttca gccgcagaat ttaagccata acgctgccac tgacgtgaaa
    21661 gcgcttcctg aaattgctta tcgttaaaaa taggttgtga cataggagtt ccacttttct
    21721 tagattttca acacaacgtt atcgctagtt tgccaggctc gatgttgacc ttcctcatcc
    21781 tgcgggggat taggcaggga ggagttgcgg ggatgagcaa ggaaatgtga tctcaaccac
    21841 ttaaagctag tgcaaaccac aggattagca gcaaatcaat gcaatacagc gcagaaaatc
    21901 tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa aagcggaagg taactctata
    21961 aattaagtaa aggagtgaaa cagtttcaca agtaaaatat ccagtgtgct ccatctcatt
    22021 cttaatagat ttattaagat catcttttta gatggcactt tcatcaggaa tgaagaagaa
    22081 acccttgctt aaatgaatct gatgaacata agggaaacca gtattcacgc tggatcagcg
    22141 tcgttttagg tgagttgtta ataaagattt ggaattgtga cacagtgcaa attcagacac
    22201 ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc cgaccttata accattaatt
    22261 acgaagcgca aaaaaaataa tatttcctca ttttccacag tgaagtgatt aactatgctg
    22321 attccgtcaa aactaagtcg tccggttcga ctcgaccata ccgtggttcg tgagcgcctg
    22381 ctggctaaac tttccggcgc gaacaacttc cggctggcgc tgatcacgag tcctgcgggc
    22441 tacggaaaga ccaccctcat ttcccagtgg gcggcaggca aaaacgatat cggctggtac
    22501 tcgctggatg aaggtgataa ccagcaagag cgtttcgcca gctatctcat tgccaccgtg
    22561 cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat
    22621 gccagcctga cgtcactctt cgcccagctt ttcattgagc tggcggaatg gcatagccca
    22681 ctttatctgg tcatcgatga ctatcatctg atcactaatc cagtgatcca cgagtcaatg
    22741 cgcttcttta ttcgccatca accagaaaat ctcaccctgg tggtgttgtc acgcaacctt
    22801 ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac tgctggaaat tggcagtcag
    22861 caactggcat ttacccatca ggaagcgaag cagttttttg attgccgtct gtcatcgccg
    22921 attgaagccg cagaaagcag tcggatttgc gatgacgttt ccggttgggc gacggcacta
    22981 cagctaatcg ccctctccgc ccggcagaat acccactcag cccataagtc ggcacgccgc
    23041 ctggcgggaa tcaatgccag ccatctttcg gattatctgg tcgatgaggt tttggataac
    23101 gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat
    23161 gcgctcatca cccgtgtgac cggcgaagaa aacgggcaaa tgcgtctcga agagattgag
    23221 cgtcaggggc tgtttttaca gcggatggat gataccggcg agtggttctg ctatcacccg
    23281 ctgtttggca acttcctgcg ccagcgctgc cagtgggaac tggcggcgga gctgccggaa
    23341 atccaccgtg ccgccgcaga aagctggatg gcccagggat ttcccagcga agcgattcat
    23401 catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg
    23461 agtttgttca accatagcga actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg
    23521 gacagtttgc tggaaaatcc gcagttagtg ttattgcagg cgtggctaat gcaaagccaa
    23581 catcgctatg gcgaagttaa caccctgcta gcccgtgctg aacatgaaat caaggacatc
    23641 agagaaggca ccatgcacgc agaatttaac gctctgcgcg cccaggtggc gattaacgat
    23701 ggtaatccgg atgaagcgga acggctggca aaactggcac tggaagagct gccaccgggc
    23761 tggttctata gccgcattgt ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc
    23821 gaattgaccc gctcactggc gctaatgcag caaaccgaac agatggcacg ccagcacgac
    23881 gtctggcact acgccttatg gagtttaatc cagcaaagtg aaattctgtt tgcccaaggg
    23941 ttcctgcaaa ccgcgtggga aacacaggaa aaagcgttcc agctgatcaa cgagcagcat
    24001 ctggaacagc tgccgatgca tgagtttctg gtgcgcatcc gagcacaact gttatgggcc
    24061 tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct
    24121 tatcagccac agcaacagct tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt
    24181 ggtgatttag ataacgcccg tagccagctg aaccgtctgg aaaacctgct ggggaatggc
    24241 aaatatcaca gcgactggat ctctaacgcc aacaaagtcc gggtgattta ctggcaaacg
    24301 accggcgata aagccgccgc tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg
    24361 aacaaccact tcctgcaagg tcaatggcgc aacattgccc gcgcacaaat cttgctgggc
    24421 gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg aaaatgcccg cagtctgcgg
    24481 ttgatgagcg atctcaaccg taacctgttg ctgcttaatc aactgtactg gcaggccgga
    24541 cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga
    24601 tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt
    24661 caactcaata cgctgccgga actggaacag catcgcgcgc agcgtattct gcgagaaatc
    24721 aatcaacatc atcggcataa attcgcccat ttcgatgaga atttcgttga acgtctgcta
    24781 aatcatccgg aagtgccaga actgatccgc accagcccgt tgacccaacg tgaatggcag
    24841 gtcctggggc tgatctactc cggttacagc aatgagcaaa ttgcgggcga gctggaagtg
    24901 gcggcaacca ccatcaagac gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac
    24961 cgtcaggccg cggtacaaca cgcgcagaaa ctgctgaaga tgatgggata tggggtgtga
    25021 gtttagccgg ataatgcatt ggatccggtt tacatcttta catcattcaa tactcacccg
    25081 cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc aagaaacgct ccaccacggc
    25141 gatattggcc agcagatggc atgaaggatg ggcgaccgta aattctcccg cgcccgccag
    25201 cgccatcggt agcaccagct ggtcggcgag atattccccc actgccgccg ggcttgccag
    25261 gtagcgtttc acctctttca ctaactgcgc ggctaccacc tcggcactga cgcgcttttc
    25321 accgacgacg aaaaagcgtt cagtgatatt ttcactttcg acttccaacg agacggtatt
    25381 acccggcccc tggtcgcgcg gcaggttatg aatattctgt tcatgcaggg aaaaactccc
    25441 cgccagtgta gcgatttcac gctcagcaac atggcgcggc acaccagcta atagaacttc
    25501 tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc aaggtgttaa acgatgccac
    25561 gggcgagact tccgttgcca ccacgccgcc tccggcagga taaaaaccgt gacgtaatag
    25621 cgtggtttgc tgatgaattc ctattttcgc cagcagcggc tccagcaccc ggcggataaa
    25681 atccgcaggc ggcgccgacg ggttatcggt gcctccgctc acttcaacac gcgaaggtcc
    25741 atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc agcgtacagc ttccggcact
    25801 accgatagca aagcggtact cgccgccgcg cacggcgcca ggccggaaga ccagacgctg
    25861 cgaccccagc tccgcgcctt ccaccgttgc cctacaaatt tccgcagccg cttttaccgc
    25921 ggtcagatgc tggcgcaaca gccccggttt cgcccgcccg gcacgaatgc tggtgatggt
    25981 aaatggctgg ccggttatca tcgacaggct cagcgccgag cgcagaatct gcccgccacc
    26041 ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc atttatcctt ttacgcacac
    26101 cacctgacgc agggtataga taacttccac cagatcgctt tgtgccgtca tcaccgcatc
    26161 aatatcttta tacgccatcg ggatttcgtc gatcacttcg gcatctttac ggcattccac
    26221 atgcgcggtg gcacgaattt gatcttccac gctgaacagt tttttcgctt tagttcggct
    26281 cattacccgc ccggcaccgt ggctgcacga acagaacgac tcttcatttc ccagcccacg
    26341 gacgataaag ctttttgctc ccatcgaacc gggaataatt ccatattgac cagcacgcgc
    26401 agacaccgcg cctttacgcg tcacgtagat ctcttcacca aagtgctgtt ctttttgcac
    26461 atagttgtgg tgacagttga tctcttccat cgccagcgtt tgtggctgtc tgaccgtttt
    26521 ctgcgtaatg ctctgcaatg ccgttaccac gttttccatc atcgcatcgc ggttaaggct
    26581 ggcaaaaagc tgcgcccagg ccacggcttt caggtaatca tcaaagtatt ccgtaccttc
    26641 cataaagtac gccagatcac gcgacggcaa cgtctcaagc gtttcctgca tctctttttg
    26701 tgccagatcg ataaagtaag tcccgatggc gttaccaatt ccgcgtgaac cggagtgcag
    26761 cataatccac acctggtccg actcatcaag gcagatttta ataaagtggt taccggttcc
    26821 cagcgttccc aggtgtttat agttattggt attcaggaaa cggggatatt tttgcgttaa
    26881 ccactgataa ccggcttcaa gctcagccca tttcgcatcg acgttaacag gcgggttttc
    26941 ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc ccgtgcggca cggccgtttc
    27001 aatcgcctga cgcagctctg ccaggttttc aggcaggtct tccgccgtta acgcggtacg
    27061 cagcgcgttc attccacagc caatatccac gcccaccgcc gccggaataa tcgccccttt
    27121 ggtcgggatc acgctaccaa tggtggaatc tttacccagg tgtacatcag gcattaccgc
    27181 aatatgtttg aaaataaacg gcatcttcgc cgtattaata agttgctgac gcgcatcggc
    27241 ttctaccggc acgcctttgg tccacatttt taccggggca ttttcagtgg tcagtaattc
    27301 gtaattcatt ttgtttttct cttttcgttg tttgctgtcc tgataattgc aaccgtcgtg
    27361 ccagaaaatt aaataagtaa attaattttt taattcattg tttttaaaaa gattatatct
    27421 ttatgtccgt aaccggagat ttcccgcaaa gccgatttac cgataatgaa atatcgtctt
    27481 ttataaggat atctaagatg cgtaaaacag tagcttttgg ctttgtcgga acggtgctgg
    27541 attatgccgg gcgcggcagt cagcgctggt caaaatggcg tccgacactc tgtttatgcc
    27601 agcaagaatc gttggtcatc gatcgactgg aattattgca tgacgcccgc tcgtgctcgc
    27661 tatttgaaac gcttaaacgc gatatcgcca gcgtttcgcc agaaacagaa gtggtgagca
    27721 ttgagattga actgcataac ccgtgggatt tcgaagaggt ttacgcctgc ctgcatgatt
    27781 tcgcccgtgg ttacaagttt cagccagaaa aagaagacta tttaattcac atcaccaccg
    27841 gcacccacgt cgcgcagatt tgctggtttc tgctggcgga agcgcgttat ctacccgccc
    27901 ggttgataca aacttcacca ccgcgcaaaa aagaacaacc gcgcggtgcc ggtgaagtga
    27961 cgattatcga tctcgatcta agccgttata acgccatcgc cagccgcttt gccgaggaac
    28021 gccagcaaac gcttgatttt cttaagtccg gcattgccac gcgtaacccc cacttcaacc
    28081 gcatgattga gcagattgaa aaagtggcga ttaaatcccg cgcaccgatc ctgcttaacg
    28141 gcccaaccgg tgcgggaaag tcatttctgg cgcgacgtat atttgaatta aaacaggcgc
    28201 ggcatcagtt tagcggcgcg tttgtggaag tgaactgcgc caccctgcgc ggcgataccg
    28261 ccatgtcgac gctgtttggt catgtgaaag gcgcgtttac cggggcgcgg gaatctcgtg
    28321 aaggtttatt acgcagcgcc aacggcggaa tgttgttttt tgatgagatt ggcgaactgg
    28381 gcgcagacga acaggcaatg ctgctaaaag ccattgaaga gaaaaccttt tacccgtttg
    28441 gcagcgatcg ccaggtgagc agtgattttc agcttatcgc cggaacggtg cgcgatttgc
    28501 gccagctggt tgccgaaggc aaatttcgcg aagatctgta cgcgcggatc aatctctgga
    28561 ccttcaccct gccgggtcta cgccagcgcc aggaagatat tgaaccgaac cttgattatg
    28621 aagtggagcg ccacgccaca ctcactggcg acagcgtgcg ttttaacacc gaagcgcggc
    28681 gcgcctggtt ggcttttgcg acctctcccc aggcaacatg gcgcggtaac tttcgcgaac
    28741 tttctgccag cgtcacgcgg atggccacct ttgccactag cggacgcatc actctggacg
    28801 tggttgaaga tgagataaac cgtctgcgct ataactggca ggagagccgc ccctccgcgc
    28861 ttacggcgtt gctgggcgca gaggcagaaa acatcgatct cttcgaccgt atgcaactgg
    28921 aacacgttat cgctatctgc cgccaggcaa agtcgctttc tgcagccgga cgacagcttt
    28981 ttgatgtttc gcgccagggc aaagccagcg tcaatgacgc ggatcggcta cgcaaatacc
    29041 tggcgcgttt tggtctgacg tgggaagccg tgcaggatca gcacagctcc agttgaatat
    29101 gatggtccgt cagcacctgc attacgctta ctggcggcgg ggcgtcggtg tagacggcat
    29161 ctaccatgct gatgctgccc atattgacca tcgcgttacg gccaaatttc gagtgatcga
    29221 caaccagcat aacgtggcgc gagttctcaa taatggcgcg cttggtgcga acttcgtggt
    29281 aatcgaactc cagcagcgag ccgtcgctat cgatgccgct tatccccaga atgccgaaat
    29341 caaggcggaa ctgggagata aaatcgagcg tcgcttcgcc aatgatcccg ccatcgcggc
    29401 tgcgtaattc gccaccggcg agaataatgc ggaaatcttc ttttaccatc aacgtgttag
    29461 caacattgag attgttggtg acaatacgca aattgctgtg attaagcagc gcgtgcgcca
    29521 ccgcttccgg cgtggtgccg atatcgataa acagtgtcga gccgttgggg atttgctccg
    29581 ccactttgcg ggcaatcctc tctttttctt cggtctgggt ggctttgcga tcgtgccacg
    29641 gcgtgttaac cgaactggaa ggcagcgccg caccgccatg gtgacgcagg atcaggtttt
    29701 gctccgccaa ctcattgagg tcgcggcgaa tagtctgcgg gctgacggag aaatgctcta
    29761 ccagctcttc agtactgaca taaccctgct gtttaaccag ttcgataata ccgttgtgac
    29821 gttgtgtttg tttcatttat aaatccctgg aattattttc gttttcgcgc attgagcgaa
    29881 tcaacaaaag ccatcgctaa acccacggct aacccggcga tgtgtgctcc gttcgccatc
    29941 gacatcccaa acaaatcaaa ccatccggcg acaatccaga tcagcgcaaa gataattaac
    30001 ccacgttgca ggtaaatgcc actttgcgga tcgcgctcac cacgtagcca gacgtagccc
    30061 atcagcgcat acaccacgcc agaaagcccg ccaaaccacg gcccgctgaa tttttgctgc
    30121 acatagccgc ttaacagggc gctaatgaga gtaataacaa ttagcttacc gctaccgagg
    30181 cgtttttcca ccgcaccgcc gagataccac caccagagca ggttaaagag gatatgcatc
    30241 agcgagaagt gcattaacgc gtgggtgaag taacgccaga actcaaattt cagtgttgga
    30301 tcgaatggcc aggccagcca taacatcact tcctgatcgc cgagaatttg catggcgata
    30361 aacaccacca cgcaggcgat catcaccacc caggttaccg gacctgcgcg ttcacgcaag
    30421 gcagcaaaga aaggataacg gcgataatgc agaccactgc cggtatggcc cgcctgccag
    30481 ctggccgcca gataacgcgg atctgccggg ttttcgagaa aacgcgccag ctccgcccgc
    30541 acgcgctcgg cctgggactc atccgccagc cagacatcgc tttggttatg ttgttgaatc
    30601 gtaaggataa caccctgcgt cgccatgtaa tcaacaaacg cctgcgccac gcgggggtta
    30661 gcaaaagagg taatcatcaa catcgttgct gtcgcttatt ccacacaaaa ggggacagta
    30721 taaagcgtta cgcgccgtac gccacctctg cgggaaactg acgctgccag gcttcaaaac
    30781 cgccgtcaat gctatagacc acatcgtagc cctgttgcag cagatactgc gccgcgcctt
    30841 tgctgctatt gccgtgataa cacatcacca tcaccggagt gtcaaagtcg ttatcacgca
    30901 taaacgcgcc cagcgtgtcg ttggttaaat ggaaagcctg caccgcatgt cccattgcga
    30961 aactctgtgg atcgcgaata tcgaccagca ccgcctcttt ttcctgcaac ttctggtgcg
    31021 cgtcggcaac gttaatacat tcgaacagat ccatgcgtct ctctttcttt acaaacaagt
    31081 gggcaaattt accgcacagt ttacgtcgaa gcggcagata aacgccataa tgttatacat
    31141 atcactctaa aatatttttt caatgttacc taaagcgcga ttctttgcta atatgttcga
    31201 tagcgaacat ttatgagctt taacgaaagt gaatgagggc agcatggaaa ccaaagatct
    31261 gattgtgata ggtggcggca tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg
    31321 tttatccgtg ctgatgctgg aggcgcagga tctcgcttgc gcgacctctt ccgccagttc
    31381 aaaactcatt cacggtggcc tgcgctacct tgagcactat gaattccgcc tggtcagcga
    31441 ggcgctggct gaacgtgaag tgctgctgaa aatggctccg catatcgcct tcccaatgcg
    31501 ttttcgcctg ccacatcgtc cgcatctgcg cccggcgtgg atgattcgga ttggtctgtt
    31561 tatgtacgat catctgggta aacgcaccag cctgccggga tcaaccggtt tgcgttttgg
    31621 cgcaaactca gtgttaaaac cggaaattaa gcgcggattc gaatattctg actgttgggt
    31681 agacgacacc cgtctggtac tcgccaacgc ccagatggtg gtgcgtaaag gcggcgaagt
    31741 gcttactcgg actcgcgcca cctctgctcg ccgcgaaaac ggcctgtgga ttgtggaagc
    31801 ggaagatatc gataccggca aaaaatatag ctggcaagcg cgcggcctgg ttaacgccac
    31861 cggcccgtgg gtgaaacagt tcttcgacga ggggatgcat ctgccttcgc cttatggcat
    31921 tcgcctgatc aaaggcagcc atattgtggt gccgcgtgtg catacccaga agcaggccta
    31981 cattctgcaa aacgaagata aacgtattgt gttcgtgatc ccgtggatgg acgaattttc
    32041 catcatcggc accaccgacg tagagtataa aggcgatccg aaagcggtga agattgaaga
    32101 gagtgaaatc aactatctgc tgaaagtgta taacgcgcac tttaaaaaac agcttagccg
    32161 tgacgatatc gtctggacct actccggtgt gcgtccgctg tgtgatgatg agtccgactc
    32221 gccgcaggct attacccgtg attacaccct tgatatccat gatgaaaacg gcaaagcacc
    32281 gctgctgtcg gtattcggcg gtaagctgac cacctaccga aaactggcag aacatgcgct
    32341 ggaaaaactg acgccgtatt atcagggtat tggcccggcg tggacgaaag agagcgtgct
    32401 gccgggtggt gccattgaag gcgaccgcga cgattacgca gctcgtctgc gccgtcgcta
    32461 tccattcctg actgaatcgc tggcgcgtca ttacgcacgc acttacggca gcaacagcga
    32521 gctgctgctc ggcaatgcgg gggcgataag cgacctcggg gaggatttcg gtcatgagtt
    32581 ctacgaagcg gaactgaaat acctggtgga tcacgaatgg gtccgccgca ccgacgatgc
    32641 cctttggcgt cgcacaaaac aaggcatgtg gctgaatgcg gatcaacaat ctcgtgtgag
    32701 tcagtggctg gtggaatata cgcagcagaa gttgtcgctg gcgtcgtaag gatgatgccc
    32761 ggttgaagtg caaccgggct atttggattc cttagactat tcattgtaaa taattgaata
    32821 acggcccctg agctaaaatc gccaaactta agcacgttac ccaatcgccc cacttcttcc
    32881 tctggtttct attttttctg caccaatggc aataatatcc caatgcaaaa ccaagctgtg
    32941 cactaatcgg gccgacaaaa tcagtccaag gctgcggcca aaaaggggaa aaattcaggg
    33001 gaggccagac aaacgtcaga aagaaatttc ggatattttc cagcaacgct gatttctctg
    33061 gagaaatata aagaatgaaa ttcgagagaa ttaaccagaa atagaccggc gaaaagacca
    33121 gccagcaatg cagtctgttt tttaattcag gaggcgttct gcgggagaat tgccaaccga
    33181 tgccagccat tacactgaaa aaaagtgcat attcaaccat taactcaagt tgtaattcct
    33241 gaagagaaat acgcacaacc aggagcggaa aggagaatat tatttctgcc agaaaagtta
    33301 acgtgaatat aagtacggct aagacaataa ccgtcggcca taccaataat ggcacgataa
    33361 caaaaccgag aattacaggg ggaatataaa gccggaatct aatcattttt attttcctgg
    33421 tgattcggat gatgcgtcat acatcgcgca taaacactca tcaattatcc agtcatcttc
    33481 ccagacctga tattctgtca ttggttcggg ctgttttagc gggttctcaa tcgcttgccg
    33541 cgctctttct ttcagtaaag gatgattatt tctagcccag gcattgattt gggtggtaat
    33601 gacgttatcg atatcgtcct ggttatctct gatataatcc tcacaatatc tgttcaataa
    33661 accggcaaga gaaacggtcg accaaaagcc gggaactgaa ttaatggcat ccagcaatgg
    33721 cgcaagagcg atatcggcac ggaaaaacat ttccagcata tactgtaatt cgacagcttc
    33781 atcgccggat acccaatcag tcatatactg gcaggcaaat cgctgtaaaa ttgcccgttc
    33841 atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa aaactacgtg
    33901 aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa aatagcggac
    33961 ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga ttaaggaata
    34021 cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac tcaggcaata
    34081 ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt gataaatctg
    34141 ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc ccgctgatcg
    34201 cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat aagggcgcta
    34261 cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct ttcctctgcg
    34321 caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt atcgcgaaat
    34381 ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taacgacctg aaagatatag
    34441 acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat cacccgctca
    34501 tccggattta gcatcgccg
//
LOCUS       DAJTNA010000020        33957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTNA010000020 DAJTNA010000000
VERSION     DAJTNA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33957
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.020"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..200
                     /locus_tag="OO161_000841"
     CDS             <1..200
                     /locus_tag="OO161_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5462007.1"
                     /translation="IRQGLGIALQPVLTLKSIAGELCSVPLEPTFYRQISLLAKEKPV
                     EGSPLFLLQMCMEQLVAIGKI"
     gene            complement(242..1318)
                     /gene="glpQ"
                     /locus_tag="OO161_000842"
     CDS             complement(242..1318)
                     /gene="glpQ"
                     /locus_tag="OO161_000842"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5462008.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1323..2681)
                     /gene="glpT"
                     /locus_tag="OO161_000843"
     CDS             complement(1323..2681)
                     /gene="glpT"
                     /locus_tag="OO161_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5462009.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2954..4582
                     /gene="glpA"
                     /locus_tag="OO161_000844"
     CDS             2954..4582
                     /gene="glpA"
                     /locus_tag="OO161_000844"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5462010.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAANPALIGAVKVPDGTVDPFRLTAANMLD
                     TKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4572..5831
                     /gene="glpB"
                     /locus_tag="OO161_000845"
     CDS             4572..5831
                     /gene="glpB"
                     /locus_tag="OO161_000845"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5462011.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLDDDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLCPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5828..7018
                     /gene="glpC"
                     /locus_tag="OO161_000846"
     CDS             5828..7018
                     /gene="glpC"
                     /locus_tag="OO161_000846"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5462012.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7211..8113
                     /locus_tag="OO161_000847"
     CDS             7211..8113
                     /locus_tag="OO161_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5462013.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8154..8957)
                     /gene="yfaU"
                     /locus_tag="OO161_000848"
     CDS             complement(8154..8957)
                     /gene="yfaU"
                     /locus_tag="OO161_000848"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR5462014.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAVGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8975..10264)
                     /locus_tag="OO161_000849"
     CDS             complement(8975..10264)
                     /locus_tag="OO161_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5462015.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALEAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10321..11525)
                     /gene="rhmD"
                     /locus_tag="OO161_000850"
                     /pseudo
     CDS             complement(10321..11525)
                     /gene="rhmD"
                     /locus_tag="OO161_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11540..12322)
                     /locus_tag="OO161_000851"
     CDS             complement(11540..12322)
                     /locus_tag="OO161_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR5462016.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHLENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12542..13744)
                     /locus_tag="OO161_000852"
     CDS             complement(12542..13744)
                     /locus_tag="OO161_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5462017.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEENLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVCFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            complement(13844..14386)
                     /locus_tag="OO161_000853"
     CDS             complement(13844..14386)
                     /locus_tag="OO161_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462018.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14665..15090
                     /gene="nudI"
                     /locus_tag="OO161_000854"
     CDS             14665..15090
                     /gene="nudI"
                     /locus_tag="OO161_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5462019.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPCQWALSGGGVEYGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15129..15731)
                     /locus_tag="OO161_000855"
     CDS             complement(15129..15731)
                     /locus_tag="OO161_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5462020.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            16039..17178
                     /gene="arnB"
                     /locus_tag="OO161_000856"
     CDS             16039..17178
                     /gene="arnB"
                     /locus_tag="OO161_000856"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5462021.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKCTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17182..18150
                     /gene="arnC"
                     /locus_tag="OO161_000857"
     CDS             17182..18150
                     /gene="arnC"
                     /locus_tag="OO161_000857"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5462022.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYRQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGDFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18150..18779
                     /locus_tag="OO161_000858"
     CDS             18150..18779
                     /locus_tag="OO161_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-L-Ara4N formyltransferase/UDP-GlcA
                     C-4'-decarboxylase"
                     /protein_id="HCR5462023.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLE"
     gene            18807..20132
                     /gene="arnA"
                     /locus_tag="OO161_000859"
     CDS             18807..20132
                     /gene="arnA"
                     /locus_tag="OO161_000859"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF005998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5462024.1"
                     /translation="MVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIA
                     PLLIACGDGALEIVTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLIL
                     GVNGFIGNHLTERLLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYH
                     VKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYG
                     MCSDKYFDEDHSNLIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMG
                     PRLDNLNAARIGSSRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIEN
                     AGNRCDGEIINIGNPENEARIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGK
                     GYQDVEHRKPSIRNAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20129..21019
                     /locus_tag="OO161_000860"
     CDS             20129..21019
                     /locus_tag="OO161_000860"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5462025.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCDYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIADSR"
     gene            21019..22671
                     /gene="arnT"
                     /locus_tag="OO161_000861"
     CDS             21019..22671
                     /gene="arnT"
                     /locus_tag="OO161_000861"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5462026.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYFLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEITQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLMLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22668..23003
                     /gene="arnE"
                     /locus_tag="OO161_000862"
     CDS             22668..23003
                     /gene="arnE"
                     /locus_tag="OO161_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5462027.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            23003..23389
                     /gene="arnF"
                     /locus_tag="OO161_000863"
     CDS             23003..23389
                     /gene="arnF"
                     /locus_tag="OO161_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5462028.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23383..23649)
                     /gene="pmrD"
                     /locus_tag="OO161_000864"
     CDS             complement(23383..23649)
                     /gene="pmrD"
                     /locus_tag="OO161_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5462029.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(23759..25114)
                     /gene="menE"
                     /locus_tag="OO161_000865"
     CDS             complement(23759..25114)
                     /gene="menE"
                     /locus_tag="OO161_000865"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5462030.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(25111..26073)
                     /gene="menC"
                     /locus_tag="OO161_000866"
     CDS             complement(25111..26073)
                     /gene="menC"
                     /locus_tag="OO161_000866"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5462031.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(26073..26930)
                     /gene="menB"
                     /locus_tag="OO161_000867"
     CDS             complement(26073..26930)
                     /gene="menB"
                     /locus_tag="OO161_000867"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5462032.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26945..27703)
                     /gene="menH"
                     /locus_tag="OO161_000868"
     CDS             complement(26945..27703)
                     /gene="menH"
                     /locus_tag="OO161_000868"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5462033.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMVACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27700..29370)
                     /gene="menD"
                     /locus_tag="OO161_000869"
     CDS             complement(27700..29370)
                     /gene="menD"
                     /locus_tag="OO161_000869"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5462034.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTTAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29459..30754)
                     /gene="menF"
                     /locus_tag="OO161_000870"
     CDS             complement(29459..30754)
                     /gene="menF"
                     /locus_tag="OO161_000870"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5462035.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIACHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30833..31138)
                     /gene="elaB"
                     /locus_tag="OO161_000871"
     CDS             complement(30833..31138)
                     /gene="elaB"
                     /locus_tag="OO161_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5462036.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31193..31654)
                     /locus_tag="OO161_000872"
     CDS             complement(31193..31654)
                     /locus_tag="OO161_000872"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5462037.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            31719..32636
                     /gene="rbn"
                     /locus_tag="OO161_000873"
     CDS             31719..32636
                     /gene="rbn"
                     /locus_tag="OO161_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5462038.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            32824..33957
                     /gene="elaD"
                     /locus_tag="OO161_000874"
                     /pseudo
     CDS             32824..33957
                     /gene="elaD"
                     /locus_tag="OO161_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8420 a         9060 c         8353 g         8124 t
ORIGIN      
        1 ttattcgaca gggattaggc atcgccttac aaccagtgtt aacgctgaaa agcattgcag
       61 gtgaattgtg ttccgttcct ctcgaaccaa ctttctatcg acagatttcg ttgctggcta
      121 aagaaaagcc ggtagaaggc agtccactgt ttttactaca aatgtgcatg gaacaattag
      181 tggcgattgg aaaaatttga tatgagacag gatggcgcat gaacaccatc ctgcagtaac
      241 attactcttt attaagaaat tttactgcct tatcagggaa atcagtaaac agcccattta
      301 cacccgcttt gttatacaga gcatcatata actgattcac atcaggagtg tattcaggca
      361 gtttatctga ccgcacggta taaggatgca ctaccagttt attctgctga gcatcttgca
      421 ccatgccagt gagtttgata ttacccggct gcgatgtctc ctcaatcaac atatggtaat
      481 ccggaccaat accatctgca tattccgcca cctgtttcat ggcacccggc ttaaacatcc
      541 agtcgtagtt gtaattaacc cagcttccat ccggctgttt ctgctgcgtt tcattccagt
      601 cggtataggc aatcagctgt accagattga gctccatgcc cattttgggt tccagctcat
      661 tcttaatacg cttcagctca tcagcatcaa aacattgcaa ataaacttta tcgtctttac
      721 cggtgtaacc atatttcttc agcacttcca gcgtttttgc cgcaatatcc ttcccttcct
      781 gatgatggaa ccacggcgct ttgatttctg gataaatacc gatatttttc ccggtagagt
      841 gatttaaccc ctgaacaaat tcaatctctt cttcaaaggt gtgcacccgg aagtcggact
      901 tacccattgg gaaacgcccc ggataagtct gcactttttt accgttttca atatcgaaac
      961 cttcggtaaa tttcaacgac ttaatttcat ccagcgtgaa atctatcgcg tagtaacgac
     1021 cgtctttgcg cgcccgatcc gggaaacgat cggcaacatc agtaacacga tcgagataat
     1081 ggtcatgcag aacaaccaga tggtcgtctt tggtcatcac caaatcctgt tccagataat
     1141 ccgctccctg cgcatacgcc atcgcttttg ctggcagcgt atgctccggc aaatatccac
     1201 tggcaccgcg atgggcgatg actatttttt cgttgctgtc cgccgccatt gcactgcttc
     1261 ccatgactat agtgctcatc atgatcgcca tgctaaggtt tttcagcgtc aatttcatgc
     1321 cattagcctc cgttgcgttt ttgtagtaat tgttcatggc gacgtttttc gccaatcatc
     1381 acaacaatca acaagataac cgccagaatg ctgccgccaa tcattaccat aaagccgcca
     1441 tcccagccga agaagtccac ggtgtagcca acaatcgcgc tcgccgccac cgaaccgccc
     1501 aggtaaccaa acagcccggt aaagcccgct gccgtacctg ccgctttttt cggtgccagt
     1561 tccagcgcat gcagaccgat cagcatcaca ggaccgtaga tcaggaagcc gataacaatc
     1621 atacaaatca tatcgacggt tgggttacct gccgggttca tccagtaaac gatagtcgcg
     1681 atggtcacca gtgtcataaa gaaaacgccg gttgccccac ggttgccacg gaagacttta
     1741 tccgacatcc agccgcacag cagagtgccc ggaatacctg catattcata aaggaagtag
     1801 gcccaggagg atttatccag cgcgaaatgc ttaacctctt tcagataagt cggtgaccag
     1861 tcgaggatgc cgtaacgcag cagataaacg aacacgttgg cgatggcgat ataccacagc
     1921 agtttgttcg gcagtacgta ctgcatgaag atttgcttcg ccgtcagctc ctgttccgct
     1981 ttttcgttat agtcgtccgg ataatcattt ttgtactctt cgatcggcgg caagccacag
     2041 gattgcgggg tatcgcgcat catcgcaaag gcgaataatg ccaccagaat ggcgcagaaa
     2101 gcaggcatat agagcgccgc atgccagtca ttgaaccagg ccatccccag caggaacagc
     2161 agcggcggaa taccaccacc aacgttgtgc gcacagttcc acactgacac aatgccgcca
     2221 cgttctttct gcgaccacca gtgcaccata gtacgaccac acggcggcca ccccatcccc
     2281 tggaaccaac cgcagaggaa caaaagtaca aacatcaccg caatgctcga cgtcgcccat
     2341 ggcacaaagc ccataaacaa catcactgcc gccgccagaa tcaaacctgc ggggaggaaa
     2401 acgcgcggat tcgagcgatc tgataccgaa cccattatga atttcgaaaa tccataagcg
     2461 atcgagatcc ccgaaagcgc gaaacctaaa tcaccgcgag agaatccctg ctcgaccaga
     2521 taaggcatag caagcgcaaa gttcttacga accaaatagt aagccgcata gccaaagaat
     2581 atccccaaga aaatttgcca gcgcaatcga cgataagtcg gatcgatctc cgcagcaggt
     2641 aagcgcgctt tgtgtggcgc tggtttaaaa atactcaaca ttgatagcct ccgtggcccg
     2701 tggtcttatt tatgattaac agcctgattc agtgagagaa cctgccgttt cttgagttgc
     2761 cgcgatgtta agaaaacatt cataaattaa atgtgaattg ccgcacacat tattaaataa
     2821 gatttacaaa atgttcaaaa tgacgcatga aatcacgttt cactttcgaa ttatgagcga
     2881 acatgctcga aatcaaacaa ttcatgtttt tactatggct aaatggtaaa aaacgaactt
     2941 cagagggata acaatgaaaa ctcgcgactc gcaatcaagt gacgtgatta tcattggcgg
     3001 cggcgcaacg ggagccggga ttgcccgcga ctgtgccctg cgcgggctgc gcgtgatttt
     3061 ggttgagcgc cacgacatcg caaccggtgc caccgggcgt aaccacggcc tgctgcacag
     3121 cggtgcgcgc tatgcggtaa ccgatgcgga atcggcccgc gaatgcatta gtgaaaacca
     3181 gatcctgaaa cgcattgcac gtcactgcgt tgaaccaacc aacggcctgt ttatcaccct
     3241 gccggaagat gacctctcat tccaggccac ttttattcgc gcctgcgaag aagcagggat
     3301 cagcgcagaa gctatagacc cgcagcaagc gcgcattatc gaacctgccg ccaacccggc
     3361 actgattggc gcggtgaaag ttccggatgg caccgttgat ccatttcgtc tgaccgcagc
     3421 aaacatgctg gataccaaag aacacggtgc cgttatcctt accgctcatg aagtcacggg
     3481 gctgattcgt gaaggcgcga cggtgtgcgg tgttcgtgta cgtaaccatc tcaccggcga
     3541 aactcaggcc cttcatgcac ctgtcgtggt taatgccgct gggatctggg ggcaacacat
     3601 tgccgaatat gccgatctgc gcattcgcat gttcccggcg aaaggatcgc tgctgatcat
     3661 ggatcaccgc attaaccagc atgtgattaa ccgctgccgt aaaccttccg acgccgatat
     3721 tctggtgcct ggcgatacca tttcgctgat tggtaccacc tctttacgta ttgattacaa
     3781 cgagattgac gataatcgag tgacggcaga agaggttgat attctgctgc gtgaagggga
     3841 aaaactggcc cccgtgatgg cgaaaacgcg cattttgcgg gcctattctg gcgtgcgccc
     3901 gctggttgcc agcgatgacg atccgagcgg acgtaacgtc agccgtggca tcgtgctgct
     3961 cgaccatgct gaacgcgatg gtctggacgg atttatcacc atcaccggtg gcaaactgat
     4021 gacctatcgg ctgatggctg aatgggctac cgacgcggta tgccgcaaac tgggcaacac
     4081 gcgcccctgt acgactgccg atctggcact gcctggttca caagaacccg ctgaagttac
     4141 cttgcgtaaa gtcatctcct tgcctgcccc gctgcgcggt tctgcggttt atcgtcatgg
     4201 cgatcgcacg cctgcctggc tgagcgaagg ccgtctgcac cgtagcctgg tatgtgagtg
     4261 cgaagcggta actgcgggtg aagtgcagta cgcggtagaa aatttaaacg ttaatagcct
     4321 gctggattta cgccgtcgta cccgtgtggg gatgggcacc tgccagggcg aactctgcgc
     4381 ctgccgcgct gccggactgc tgcaacggtt taacgtcacg acgtccgcgc aatctatcga
     4441 gcaactttcc accttcctta acgaacgctg gaaaggcgtg caacccatcg cctggggaga
     4501 cgcactgcgc gaaagcgaat ttacccgctg ggtttatcag ggactgtgtg gtctggagaa
     4561 ggagcagaaa gatgcgcttt gatactgtca ttatgggcgg cggcctcgcc ggattactct
     4621 gtggcctgca actgcaaaaa cacggcctgc gctgtgccat tgtcacgcgt ggtcaaagcg
     4681 cgttgcattt ctcatccggt tcgctggatt tgctgagcca tctgccagat ggtcaacccg
     4741 tggcagacat tcacagtgga ctggaatctt tgcgtcagca ggcacctgca catccttact
     4801 ctcttctcgg gccacaacgc gtgctcgatc tcgcttgcca ggcgcaggca ttaatcgctg
     4861 aaagcggtgc gcaattgcag ggcagcgtag aactggctca ccagaggatt acgccgctcg
     4921 gcactctgcg ctctacctgg ctaagttcgc cagaagtccc cgtctggccg ttgcccgcga
     4981 agaaaatatg tgtggtcggg ataagcggcc tgatggattt tcaggcgcat cttgcggcgg
     5041 cttcgttgcg cgaactggat ctttcggttg aaaccgcaga aatagagctg ccggaactgg
     5101 atgtcctgcg caataacgcc accgaatttc gcgctgtgaa tatcgcccgt ttccttgata
     5161 atgaagaaaa ctggccgctg ttacttgatg cgcttattcc tgtcgccaat acctgcgaaa
     5221 tgatcctgat gcctgcctgc ttcggcctgg acgatgacaa actgtggcgt tggttgaatg
     5281 aaaaactacc ttgttcactg atgcttttgc caacgctgcc gccttccgtg ctgggcattc
     5341 gtctgcaaaa ccagttacag cgccagtttg tgcgccaggg tggcgtgtgg atgccgggcg
     5401 atgaagtgaa aaaagtgacc tgtaaaaatg gcgtagtgaa tgaaatctgg acccgcaatc
     5461 acgccgatat tccgctacgt ccacatttcg cggttctcgc cagcggcagt ttctttagtg
     5521 gcggactggt agcggaacgt aacggcattc gcgagccgat tctcggcctt gatgtgctac
     5581 aaaccgccac gcggggtgaa tggtataagg gcgatttttt tgcgccgcaa ccgtggcagc
     5641 agttcggtgt aaccactgat gagacgctat gcccgtcgca ggcagggcaa accattgaga
     5701 acctgtttgc catcggttcg gtgctgggcg gatttgatcc catcgcccag ggatgcggcg
     5761 gcggtgtttg tgccgtcagt gctttacatg ccgctcaaca gattgcccaa cgcgcaggag
     5821 gccaacaatg aacgacacca gcttcgaaaa ctgcattaag tgcaccgtct gcaccaccgc
     5881 ctgcccggtg agccgggtga atcccggtta tccagggcca aaacaagccg ggccggatgg
     5941 cgagcgtctg cgtttgaaag atggcgcact gtatgacgag gcgctgaaat attgcatcaa
     6001 ctgcaaacgt tgtgaagtcg cctgcccgtc cgatgtgaag attggcgata ttatccagcg
     6061 cgcgcgggca aaatatgaca ccacgcgccc gtcgctgcgt aattttgtgt tgagtcatac
     6121 cgacctgatg ggtagcgttt ccacgccgtt cgcaccaatc gtcaacaccg ctacctcgct
     6181 gaaaccggtg cggcagctgc ttgatgcggc gttaaaaatc gatcatcgtc gcacgctacc
     6241 gaaatactcc ttcggcacgt tccgtcgctg gtatcgcagc atagcggctc agcaagcaca
     6301 atataaagac caggtcgctt tctttcacgg ctgcttcgtt aactacaacc atccgcagtt
     6361 aggtaaagat ttaattaaag tgctcaacgc aatgggtacc ggtgtacaac tgctcagcaa
     6421 agaaaaatgc tgcggcgtac cgctaatcgc caacggcttt accgataaag cacgcaaaca
     6481 ggcaattacg aatgtagagt cgatccgcga agctgtggga gtaaaaggca ttccggtgat
     6541 tgccacctcc tcaacctgta catttgccct gcgcgacgaa tacccggaag tgctgaatgt
     6601 cgacaacaaa ggcttgcgcg atcatatcga actggcaacc cgctggctgt ggcgcaagct
     6661 ggacgaaggc aaaacgttac cgctgaaacc gctgccgctg aaagtggttt atcacactcc
     6721 gtgccatatg gaaaaaatgg gctggacgct ctacaccctg gagctgttgc gtaaaatccc
     6781 ggggcttgag ttaacggtgc tggattccca gtgctgcggt attgcgggta cttacggttt
     6841 caaaaaagag aactacccca cctcacaagc catcggcgca ccactgttcc gccagataga
     6901 agaaagcggc gcagatctgg tggtcaccga ctgcgaaacc tgtaaatggc agattgagat
     6961 gtccacaagt cttcgctgcg aacatccgat tacgctactg gcccaggcgc tggcttaaac
     7021 tcctttctga tgcccggtaa gcatgtggtt accgggcatt tttgcgtaca cgattccgtg
     7081 cccaatgtat gcgttgcaac gcagtgaaaa ttcctctgaa aacgtctcgc aaaggctgaa
     7141 actggcagat gtcaaaggcc tgggataacc gtaatgtcac gtcatcataa atatcaggtg
     7201 acggacaacc atgaccgaat caacaacctc ctccccgcat gatgcggtat ttaaaacctt
     7261 tatgttcaca cccgaaaccg cacgggattt tctcgaaata catttaccag aaccactgcg
     7321 caagctttgc aacctgcaaa ccttacgcct ggaacccact agttttattg aaaaaagttt
     7381 acgcgcttac tactcggatg ttttgtggtc cgtggaaacc agcgacggtg acggctatat
     7441 ctactgcgtg attgaacatc aaagctctgc agaaaagaat atggcttttc ggctaatgcg
     7501 ctatgccact gccgccatgc agcgtcacct ggataaaggc tatgacagag ttccgctggt
     7561 ggtgccattg ctgttttatc atggcgaaac atcgccctac ccgtactcac ttaactggct
     7621 ggatgagttt gacgatccgc aacttgcccg gcagttgtac accgaagctt ttccgttggt
     7681 ggatatcacc atcgtacctg acgatgagat catgcaacat cggcgtatag ctctgctgga
     7741 actgattcaa aagcatattc gcgaccgcga tttaatcggc atggtcgaca ggatcaccac
     7801 gcttttggtt agaggcttca ctaatgacag ccagctacaa acactgttta attatctgct
     7861 gcaatgcggc gatacctccc gtttcacccg ttttattgag gagattgccg aacgttcacc
     7921 actacaaaag gagagattaa tgactattgc tgaacggcta cggcaggaag ggcatcaaat
     7981 tggctggcag gaaggtatgc atgaacaagc cattaaaatt gctttgcgca tgctggaaca
     8041 gggcattgat cgtgaccagg tgctcgcggc cacccagcta agcgaagccg atctggcagc
     8101 gaataaccac taattaacac aggcccacag ccgatcccca tgggcctttg atatcaataa
     8161 ctacctttta tgcgtggccc atttttgcct gatttaaaca tcgccagtcg ttgatccagg
     8221 gcatcgctgt agagcatcgt gtcaacgcca acagcgacaa agttcgctcc ccacgccagg
     8281 cattgctgcg ccatatcagg agccacagcc agaaaaccag ccgctttacc cacagcacgg
     8341 atccgccgaa tgctggtttc aataattcgc tgcacttccg ggtgcccggc gttatccggg
     8401 tagcccaacg acgcagaaag atccgctggt ccaataaaca cgccatcaat cccttcgacg
     8461 tcgaggatct cgtccaggtt atccagtgcc gttttacttt ccacctgcac taacagacaa
     8521 agcgaatcgt tgacttgcgc catgtaattc tcaatgcgtc cccagcgcgc agcccgtgcc
     8581 acactggccc caacaccacg ctcaccgtag ggaggatagc gcgtggcaga cgccacctga
     8641 cgagcctgat cggcagtatc gaccatcggg atcaatagag tttgcgcgcc aatatccaga
     8701 acttgtttaa tcagcgattt actgccttcc accggacgga tcacgggttg gctggcatag
     8761 ggcgctaccg cctgtagctg gtgataaaga tcctgaatgg tgtttggcgc gtgctccccg
     8821 tcaatcagca accagtcata accggaagta gcggcaattt ctgccatata agaagtagtc
     8881 gagcttaacc acagaccaat ttgcacttcg cccttgcgta aacgttcttt aaagggattg
     8941 gttaataatg cgttcatcgt gttccttaac ctatttaatg atgtgccacg tcggtctgcg
     9001 caacagtacg atttacacgc agagtgaaaa taatcagcga accgatgacc gccaccgctg
     9061 ccagtgtcag taatcctgcc gcatcgctgg caaataacgt ttctgctttc acgcgcagga
     9121 tcggggcaat aaacccgccc actgcaccaa acaggtttac aaaaccaatc cccgcagcca
     9181 gcgccgtacc ggaaagcagt tgtgtcggca tcgtccagaa caccggctgc acggcaataa
     9241 acccgatagc tgcaacacac agcgctacga tcgccattac tggagaaagc agcccggaca
     9301 gaccaatacc aatgcccgcc gccagtaatg tcagcgctgc gacattacgc cgttcgccgg
     9361 ttttatcgga atagcgcgga ataagccagg tcccaaacaa ggccgcaacc cacggaatgg
     9421 cggtgaccac cgacgctgta aagcccactt ttgttcccag caatgccgca acctgggtcg
     9481 gcaggaagaa aattaatccg taaaccgcca cctgaatggt caggtagata atcgctagtt
     9541 gccagactcg gccattacgc agcgcatcgc tcagccgaga agtcactttc tgttgttctt
     9601 cacttgccag ttgattgata agcaacgttt tttcttgttt actcagaaaa cgtgcctgct
     9661 ccggtgtgtc atcaagccaa aagaatgtga ataccccagc accgactgcc aacaatcctt
     9721 caatcacaaa catccagaac cagccgggat gccccataaa tccatgcatc tccaacagcg
     9781 cgccggaaag cggtgatccc agtgttaacg ccagcggtgc gcccatatag aacagcccca
     9841 taatgctggc gcgattacgc tgcggaaacc attgcgaggt gagataaatc ataccaggga
     9901 aaaatccggc ctccgcagca ccaagcagag tgcgaactat cagaaatttc gcttcagtat
     9961 cggcccatgc catggctgcc gaaagaaatc cccacagcag tgttgtcgta ccaatccagg
    10021 ttctggcccc cagtttgcgc atcaaaagat tcgccggaac acccagaaac gcatatacca
    10081 caaagaaaat gcctgcttcc agcgcataag cttcattact caacccggta tcaatctggt
    10141 aggtctgttt ggcaaaaccg atattcgaac ggtcgagaaa cgccagcaca tacagcgcca
    10201 acataaacgg aattaaacgc acacggtttt tcttcaccac ggcgtcaagc aaagcggtgc
    10261 tcataaaaag ctccttagaa tatgtgggcg ttaccgttgt cacagcaacg cccggagtga
    10321 ttagtggctg taggggcgtt tcaaattgca gtcacgattg agttcgacgc caaaaccggg
    10381 tttatcaagc actgatttat gaatacgacc attcaccgga accggctcat tgagcagaat
    10441 cgggtcaaac tgcggacgca tcgttgaaca atccgggctg gtcatcagga attcgctgaa
    10501 tggcgtattg gtgaaggtga tcaccgcatg gtgagagtaa acagacgaac cgtgcggcac
    10561 caccagttgc cccctggatt tggcgattgc ggcaatttcc accagcgtgg taaaccaccg
    10621 caccagccaa catccggctg cataatgtcg ataccggttt ctgaaagcgt acgaaaagat
    10681 tgcagtgtgc cgtggtgctc accgctggtg accatcattc cgactggcgc gttgcgtttc
    10741 agttcgcgat aaccttcata ctgctgtggc ggcaggcact cttcgatcca tttcaggtta
    10801 tagggcgcgc aagcgtgggc tagtttggtc gcatagttca cgtcctgact catccagcag
    10861 tcgagcatta accagaaatc ttcaccgcat ttttcacgca tatccgcgac catagcggca
    10921 tctttgcgga tccccgcatc gccatcatgt ggcccccagt gcgtcggcat tttgccaccg
    10981 ataaagccca tctcttttgc cagatccgga cgcgcacctg tggcgtagaa ctgaatctca
    11041 tcacgaacag cgccgcctaa aagtttataa accggaagcc cgaccacttt gccgaacaga
    11101 tcccacagag ccagatcgac acaggaaatc gtattcatca ccaagccacc agagccggag
    11161 taatacaggg tggcattgag catttgatcg tggatcagtt tgatatcact gacacattta
    11221 ccctcaatga aacagttaag gtgtttttcg acgataaaac agcccatttc accggctgtc
    11281 gaaacagcga atccggtctg gccgttttct gcttcgactt caacaaccaa cgtgccaaga
    11341 acgttaatgc caaatgactg gcgtgactgc tcgtaatcgc ggtatttact catcggggtg
    11401 gcaatatgat cgtctatcca gtgattcgcc ccctggtcgt tataatcacc gccgccagcg
    11461 cctttttctg ctgtcgcacc gccagtaaac caggcgcgaa cctgtttaat ttttggtagg
    11521 gtcatgatgt tctccattgt tatgaggctt gtaagtcaaa gggacttttc catcccaaca
    11581 gacgtgaaat atccctggcg caggcaatgg ccttgcccgc cagataatca cggtattctt
    11641 cattgatttg taagcgggta ccgaccaccg agatcgcagc ggtaagctcg ttattggcgt
    11701 taaacaccgg cgcagcgaca caacggacat cggggtaatc ttcgccgttg tcatagctcc
    11761 agccctgacg gcgaatacgc gccagttctt cgtgaagttg ctgtggatga gtaatcgttg
    11821 tgggtgtcgc ctgctcccag accagccctt cgataatact ttgctgtacc gctgcaggtt
    11881 gccaggcaag caggcattta cctattccgg agcgataaag cgaaaggctt tttccttcat
    11941 gggaacgcac gctgatagtg gctgatgact ccacttttaa aatgtaataa gcgctgccgt
    12001 tatcaataat ccccagatga cataacagcc cggtggtatc catcagttgc gtcaaacgcg
    12061 gtcgagccag ttcccggaga tccatcttgc ttaacgcatg accagaaagc tccaccagcc
    12121 tggtccacaa acaaaaattt tccaggtgat caaggctaag aaaacgctga cgcctgagtt
    12181 cattaagcag caaataggcc gtgcttttag ggattcccaa tgtgtcaatg atcgtcgcag
    12241 cactacaggg gccaatgcgg gcaatcaaat tgagaatatc aatagcccgt gtcagggcgg
    12301 gaactttgct tgattccaac atactggact ccagtcttag atactggaat cagtctcgtt
    12361 gctcaggggg agtaaaattg tgaagcgcat caaatcccgc tcctcgtcca gcggactgga
    12421 gaaattacgc acaaatttgt gaatcagtgc acggattttt tcttattgag tgcaacaggt
    12481 tggacaagtt ttgccggagg gggaatgtgc cggtgcagta aaccggcaca gagtggcgga
    12541 atcagacaga taaggtcatg gactcaataa cctcaatcca gccatgctca ctggcgatat
    12601 cctggccgtt taaccaacgg cgcagcatat tcagtgccat cattgcgcac acttcctgac
    12661 ggatagccag gctgtagcgc gtagtgctga aacacacacg cagagcgaaa gtgccgtctg
    12721 gtgtggctag cgcaaagttg agatgttcgt tctcgaaacc cgaaacagcc agtgccagcc
    12781 cggcaaaatg gttggcccgc cgttctgtaa tccagtgcgc agtttgcgcc aggttttctt
    12841 cctgtgaagg aaccacttca cacgccagca atggagcacc tgcgcgagaa agttgcaaag
    12901 ccaataaacc accggtgaat tgctcgctca acgtcaggct gaactggcgg ttttgcaatt
    12961 cgcgactgat ctgcgcgggc agtccttcgg tgccttcgaa aatcacgctc tgtccggcaa
    13021 cacgtttaac gtccagccac agtttttcca tcgcctgttg ctcgcttgcc ggtccggtga
    13081 gtttcagttc gataatcggc attgaagagc gatagcccat tgttacgccc ggcggcagtt
    13141 gtagagtgtc caggctttgt gccagatcgc tttccgaacg accaaaggta gttaaacgca
    13201 gacaaaccgg cggctgtggt aaagaaaaac gctcgcgcag gcgcggcagg atttcgtgct
    13261 cgaccatcac cttaaattct gacggtacgc cgggagtaaa gaacatcagg caacgattaa
    13321 gctgcacggc aaaaccacag gcggtgccta ccgggttatt gataaattca gcactggcag
    13381 gcagctccgc ttgtttacgg ttgctcggtg ccattactcg tccacgttcg tggaaatagc
    13441 gttccatctc tttgagccag gcttcatgca gcaccaggcc ttcacctttt gctgtcgcag
    13501 cggcgagtgc gcttaaatca tcgctggtcg gtcccagccc gccgttaacg atcagcacat
    13561 cggcgtgctg actacgttcg cgaagaatgg tgactaagtc atcaaggtta tcccccaccg
    13621 tattgcggcg agataatggc agcccctgat gaaagaaaaa atcggccagc caggcagcgt
    13681 tagtgtcaac gatttgcccg tgtaacactt catccccggt ggataacatt tccactttta
    13741 acatggttat ctcctgcttt tgttatcgaa tcactataac gcaaagggag ataaggcgta
    13801 gtaagaaact gaatggcgcg acagaatgcc gcgccggaag ggatcagaaa ctggcgttaa
    13861 ccccaacata cgggccgtca gccacggcgt tgtcgcggtt accatcttta cccgacaaat
    13921 tcaggtagcg ataacccgcc tcaatactga ctggacgcat aatggtgtaa cgcgcgccag
    13981 cattcgcttc ctcataactt tgaataccgc tggagagtga atccggagag tagtaatact
    14041 cgccaaacaa acggaagctg ttgccaattt tccactgcaa accacctcct accgctgcgg
    14101 cataaccttc atcgccgtaa ttcgggttgg tgtaaacgcc ttttccgcca acggtcgcca
    14161 ttaacggccc tagaggcaga ttcaacccca gccccacgcc agcgacgtca ccgtcgtcgt
    14221 cgttatgtgt ccagttaccg cttaacgcca ggcccgtcga ttcagtccca aaaccgacac
    14281 caatattggt gtattcttta cccgcctgac cgctgatgct cattgcattg accgatgcag
    14341 aaaccaacag cattccggcc agacctgcta gcgcaatttt tttcattatc ccctttccca
    14401 caagcaatta atcaaaattc ccaaagctgc gggattgtac tgcctggcac agaggattca
    14461 atgagcgaaa aaagggatct agtcacttat tgtaactaaa tacttcttaa ataattaaac
    14521 gccgaaaaaa taaacacact ttaccgatac acacaaaaat aataataagt gcagttatca
    14581 tcgcaaaaga gattactgtc actttcacat atttctgagt taccgtattc tcatggtttc
    14641 tttttattta taaggaatac ccctgtgcga caacggacta ttgtatgccc tttgattcaa
    14701 aatgatggtg cttatctgct gtgcaaaatg gccgacgatc gcggcgtttt cccctgtcaa
    14761 tgggcgcttt cgggtggcgg cgtggagtat ggcgaacgaa ttgaagaggc actacgccgc
    14821 gaaattcgcg aagaactggg agaacagctg cttttgacag aaatcacgcc gtggaccttc
    14881 agcgatgata ttcgcaccaa gacgtatgca gatggtcgca aggaagagat ttatatgatt
    14941 tacctgattt ttgactgcgt ttctgccaac cgagaagtga aaataaacga agagtttcag
    15001 gactacgcgt gggtaaaacc tgaagatctg gtgcattatg atttgaatgt tgccacccga
    15061 aaaacgttac gtttgaaagg tcttctttaa caatgatgac agccatcact ggctgtcaga
    15121 ttgaaaattt agtggttaac gaattcccca tccagataaa ctttgccttt ctccacatgc
    15181 atgactaaac catccagata atcaggttta aatgtcgcat cacgcttatt tttagcaata
    15241 tatgtcaggc aatgattatg ggtgaaaata acgatatttt tatcaggcgc tttgctttgt
    15301 aagtccttaa ttgcactata aatctcatta ccgcactgaa gaagtcgttt atctaccgtc
    15361 agctttttac ccgcggaaaa ccaggtcgcc gactgaatgg tccggacggt attactggaa
    15421 taaagatcga aatcagggat gtcagcgcta aaagcgttgc ccagttcacg ggcatcctgg
    15481 gtacctttaa ccgtaatacc tgttttatct gacaagcatt gattggttga acgatcgcaa
    15541 cgttcagcat ggcgaaacaa aacgacaacc gggtgctgct gtgccagtct ggccaatgtt
    15601 ttattgtcga tacgtggcaa accattgcta ctccaggcgg catgagtacc cagtccggca
    15661 attgcagcga gcgccagtaa aatgatgaaa tatttttttg acttcaatga tgagcggcaa
    15721 aaagctaaca cgtaaactcc acctatagac aagcgcaacc tgacaattac cgtgaaattg
    15781 agcgacattt ctggcgataa ttagcagttg gtgtaatatt aaaaatccta cgatgtcggc
    15841 aaaatgcctc aaaattttgc caaatgcaaa gcctaaataa gaaaaaatat aaaaacttca
    15901 atatttacgt ctaatattag tttcttaagg ttaagttaat attctatcct taaattttcg
    15961 ctccaaatgg caaaatatac acaacactct ttatagcaaa tataagtgga caggtattca
    16021 atggcggaag gaaaagcaat gtcagaattt ttgccttttt cgcgaccagc aatgggcgtg
    16081 gaggaactcg ctgcagttaa agaggttctc gaatccggtt ggatcacaac cggtccgaag
    16141 aatcaggcgc ttgagcaagc cttttgccag ttgacgggaa atcagcatgc catcgcggtc
    16201 agttcagcca ccgccggaat gcatatcacg ctaatggcgt tggaaattgg caagggcgat
    16261 gaagtgatta cgccttccct gacctgggtt tcaaccctca atatgatttc cttgttgggt
    16321 gcaacgccgg taatggtgga tgtcgaccgc gatacgctga tggtcacgcc tgaagcgatc
    16381 gagtcagcca ttacgccacg cactaaagcc atcattccgg tgcattatgc cggtgcgcca
    16441 gcagatattg acgccattcg cgccattggc gaacgttacg gcatcgcagt tatcgaagat
    16501 gctgcccatg ccgtcggtac gtattacaaa gggcgacata ttggcgcaaa atgtaccgct
    16561 attttttcat ttcatgccat taaaaatatt acctgtgcgg aaggtggcct gattgtaact
    16621 gataatgaaa accttgcccg ccagctacgg atgctgaaat ttcacggcct gggtgtcgat
    16681 gcctatgaca gacaaacctg gggccgtgca ccgcaggctg aagtcttaac accgggctat
    16741 aagtacaatc tgaccgatat taacgccgcg attgccctga cacagttagc aaaattagag
    16801 cacctcaaca cccgtcggcg cgaaattgcc cagcaatatc agcaagcact ggcagctctc
    16861 ccctttcagc cattaagcct tcccgcctgg ccgcacgttc acgcctggca tctgtttatt
    16921 attcgtgtcg atgaacaacg ttgtggtatc agtcgcgatg cgttgatgga agcgttaaaa
    16981 gaaagaggca ttggtaccgg gttacatttc cgcgccgctc acacacaaaa atattatcgc
    17041 gagcgttttc ccacgctgtc gttaccgaat accgaatgga atagcgaacg catctgttct
    17101 ttgccgctgt tcccggatat gactaccgca gatgccgacc gtgtcatcac agcccttcag
    17161 caactcgcag gacaataagc catgtttgaa atccaccctg ttaagaaagt ctcggtggtt
    17221 attcccgttt ataacgagca ggaaagctta ccggaattaa tcaggcgcac caccacagcc
    17281 tgtgaatcgt tggggaaaga gtatgagatc ctgctgattg atgacggcag tagcgataat
    17341 tccgcgcata tgctggtcga agcctcacaa gcggagaaca gccatattgt gtctattttg
    17401 cttaaccgca attacaggca acattcagcg attatggcgg gattcagtca cgttactggc
    17461 gacttaatta ttacccttga tgccgatctc cagaatccgc cagaagaaat cccccgccta
    17521 gtggcaaaag ccgatgaagg ttacgacgtg gtagggactg tacgccagaa ccgccaggac
    17581 agctggtttc gtaaaacagc ttcgaagatg attaaccggc ttattcagcg caccaccggc
    17641 aaagcgatgg gtgactacgg ttgtatgctg cgcgcctatc gccgtcatat tgtcgatgcg
    17701 atgttgcact gccatgaacg tagcaccttt atcccgattc tggcgaatat cttcgcccgc
    17761 cgtgccattg aaattccagt acatcatgcc gagcgtgagt ttggtgaatc caaatacagt
    17821 tttatgcgcc tgattaattt gatgtacgac ctggtgacct gccttaccac aacgccgcta
    17881 cgtatgctga gtctgctcgg cagcattatt gcgattggag attttagcat tgcggtgctg
    17941 ctggtgattt tacgcctgac cttcggacca caatgggcgg cagaaggcgt ctttatgcta
    18001 tttgccgtgc tgtttacttt tattggcgct cagtttatcg gcatgggatt actcggtgaa
    18061 tatatcggca ggatctacac cgatgtccgc gcccgccccc gctattttgt tcagcaagtt
    18121 atccgtccat ccagcaagga aaatgaataa tgaaaaccgt cgtttttgcc taccacgata
    18181 tgggatgcct cggtattgaa gctctgctgg ctgccggtta cgaaattagc gccattttta
    18241 cccataccga taatcccggt gaaaaagcct tttatggttc ggtggctcat ctggcggcgg
    18301 aaagaggcat tccggtttat gcgccggata acgttaatca tccgctgtgg gtggaacgca
    18361 ttgcccaact gtcaccagag gtgattttct ctttttatta tcgccatctt atttgcgacg
    18421 agattttgca gctcgctccc gcaggtgcat ttaatctgca cggttcgctg ttaccaaaat
    18481 atcgtggtcg cgcgccgctg aactgggtgc tggtgaacgg tgaaacggaa acgggcgtta
    18541 cattgcaccg aatggtaaaa cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg
    18601 ccattgcgcc agacgatatc gctattacgc tgcatcataa attgtgccat gccgcgcgcc
    18661 agctactgga acagacatta cccgccatta aacacggtaa tattctggaa atcgcccagc
    18721 gcgaaaacga agccacctgt tttggtcgca gaacgccgga tgacagtttc cttgaatgac
    18781 ataaaccggc atccgtactg cacaacatgg tacgtgccgt tgccgatccg tggccgggtg
    18841 ccttcagcta tgttggcaat cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg
    18901 ccagcaaagc acagccgggg agcgtgattt ctattgcacc gctgttgatt gcctgtggcg
    18961 atggcgcgct ggaaatcgtc accggacagg cgggcgacgg cattactatg cagggctcgc
    19021 aattagcgca gacgctgggc ctggtgcaag gttcacgctt gaatagccag cctgcctgca
    19081 ccgcccgacg ccgtacccgg gtactcatcc tcggggtgaa tggctttatt ggcaaccatc
    19141 tgacagaacg cctgctgcgc gaagatcatt atgaagttta cggtctggat attggcagcg
    19201 atgcgataag ccgttttctg aatcatccgc attttcactt tgttgaaggc gatatcagta
    19261 ttcattccga atggattgag tatcatgtca aaaaatgtga tgtcgtcttg ccgctggtgg
    19321 cgatagccac gccgattgaa tatacccgca acccgctgcg cgtatttgaa ctcgattttg
    19381 aagagaatct gcgcattatc cgctactgcg tgaagtaccg taagcgaatc atcttcccgt
    19441 caacttcaga agtttatggg atgtgtagcg ataaatactt cgatgaggac cattctaatt
    19501 taatcgtcgg cccggtgaat aaaccacgct ggatttattc ggtatcaaaa caattacttg
    19561 atcgggtgat ctgggcctat ggcgaaaaag agggtttaca gttcaccctc ttccgcccgt
    19621 ttaactggat gggaccacga ctggataacc ttaatgcggc acgaatcggc agctcccgcg
    19681 ctattacgca actcattctc aatctggtag aaggttcacc gattaagctg attgatggcg
    19741 gaaaacaaaa acgctgcttt actgatattc gcgatggtat cgaggcgtta taccgcatta
    19801 tcgaaaatgc gggaaatcgc tgcgacggtg aaattatcaa cattggcaat cctgagaacg
    19861 aagcgagaat tgaggaactg ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc
    19921 gccatcattt cccaccgttt gcgggctttc gcgttgtcga aagtagcagc tactacggca
    19981 aaggatatca ggacgtagag catcgtaaac cgagcatccg caatgcccac cgctgcctgg
    20041 actgggagcc gaaaattgat atgcaggaaa ccatcgacga aacgctggat ttcttcctgc
    20101 gcaccgttga tcttacggat aaaccatcat gaccaaagta ggcttacgca ttgatgtcga
    20161 tacctttcgt ggcacccgtg aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa
    20221 tattcaggcc agcatttttt tcagcgtcgg cccggacaat atgggccgcc atctctggcg
    20281 actggtgaag ccacagtttt tgtggaagat gctgcgctca aacgcggcat cgctttatgg
    20341 ctgggatatt ttactggcag gtacggcctg gccaggtaaa gagattggtc atgccaatgc
    20401 cgatatcatt cgtgaagcgg ctaaacatca cgaagtcggc ctgcacgcct gggatcacca
    20461 tgcctggcaa gcccgtagcg gtaactggga tcggcaaaca atgatcgacg atattgcacg
    20521 cggtcttcgc actctggaag agattatcgg tcaaccggta acctgttctg ccgctgcggg
    20581 ctggcgtgcc gaccagaagg tgatcgaagc aaaagaagcg ttccatttgc gctacaacag
    20641 cgattgtcgt ggggccatgc cgttccgtcc attgctcgaa tcaggaaacc ctggcactgc
    20701 gcaaattccg gtgaccttac ccacctggga tgaagtgatt ggtcgggatg tgaaaacaga
    20761 agattttaac ggttggttac tcaaccgcat cctgcgagat aaaggcacgc cggtttatac
    20821 cattcatgca gaagttgaag gctgcgatta tcagcataat tttgtggatc tcctcaaacg
    20881 cgcagctcag gaaggcgtga cattttgccc tttaagcgaa ctgttatcag agacgttgcc
    20941 gctcggacaa gttgttcgcg gaaatattgc cggacgtgaa ggctggctgg gttgccaaca
    21001 aattgcggat agtcgctgat gaaatcggta cgttacctta tcggcctctt cgcgtttatt
    21061 gcctgctatt acctgttacc gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt
    21121 tatgcggaaa tcagtcgaga aatgctggca tccggcgact ggattgtgcc ccatctgtta
    21181 gggctacgtt atttcgaaaa acccattgcc ggatactgga ttaacagcat tgggcaatgg
    21241 ctatttggcg cgaataactt tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact
    21301 gccgcgctgg tgacctggtt tactctgcgc ttatggcgcg ataaacgtct ggctctactc
    21361 gccacagtaa tttatctctc attgtttatt gtctatgcca tcggcactta tgccgtgctc
    21421 gatccgttta ttgcattctg gctggtggcg ggaatgtgca gcttctggct ggcaatgcag
    21481 gcacagacgt ggaaaggcaa aagcgcagga tttttactgc tgggaatcac ctgcggcatg
    21541 ggggtgatga ccaaaggttt tctcgccctt gccgtgccgg tattaagcgt gctgccatgg
    21601 gtagcgacgc aaaaacgctg gaaagatctc tttatttacg gctggctggc ggttatcagt
    21661 tgcgtactga cggttctccc ttggggactg gcgatagcgc agcgggagcc tgacttctgg
    21721 cactattttt tctgggttga gcatattcaa cgctttgcac tggatgatgc ccaacataga
    21781 gctccgttct ggtactacgt gccggtcatc attgccggta gcctgccgtg gctgggatta
    21841 ctccccggtg cactgtacac aggctggaaa aaccgcaagc attccgcaac cgtctatttt
    21901 ttgagctgga cgataatgcc gctgctgttt ttctccgtcg ctaaaggtaa attgcccacc
    21961 tatattcttt cctgctttgc atctctggca atgctgatgg cgcattacgc tttgctggca
    22021 gcaaaaaata atcctctggc gctgcggatt aatggctgga ttaacatcgc ttttggcgtc
    22081 actggcatta ttgccacatt tgtggtctcc ccgtggggac caatgaacac gccggtgtgg
    22141 caaaccttcg agagctataa agtcttttgt gcctggtcga ttttttcgct atgggcattt
    22201 ttcggctggt acaccttaac aaacgtcgaa aagacctggt ctttcgccgc gctttgcccg
    22261 ctggggctgg cgttgctggt aggattttca attcctgaca gagtcatgga aggaaaacat
    22321 ccgcaatttt ttgtcgagat aacacaagaa tcacttcaac caagccgcta tattcttact
    22381 gatagcgtcg gtgttgccgc aggtctggca tggagcctgc aacgcgatga catcatcatg
    22441 tatcgccaga caggtgagtt gaaatacggc cttaattatc cggatgcgaa agggagattt
    22501 gtcagcggtg atgagttcgc aaactggctt aatcaacatc gtcaggaggg gattattaca
    22561 ctcatgcttt cggttgaccg cgatgaagat atcaacagtc tcgccattcc gcccgcagat
    22621 gccatcgatc gtcaggagcg tctggtgctg attcagtatc gtcccaaatg atctggctaa
    22681 cattagtctt tgccagcttg cttagcgttg ccgggcagtt gtgtcagaaa caggcaacct
    22741 gctttgtggc gataaacaaa cggcgcaaac atatcgtgct gtggctggga ctggcgctgg
    22801 cttgtcttgg tcttgccatg gtgctctggc tgctggtctt gcagaacgta ccggtaggca
    22861 ttgcctaccc gatgttaagt ctgaattttg tctgggtgac gctggctgca gtaaaactgt
    22921 ggcacgaacc ggtatcgccg cgtcactggt gtggggtggc gttcattatt ggtggcattg
    22981 tgatcctcgg gagtacggtg taatgggcct gatatgggga ttattcagcg tcattattgc
    23041 ctcagttgcg caattaagcc tgggttttgc ggcaagtcat ctgccgccga tgacgcactt
    23101 atgggatttt atcgcggctc tgttagcttt tggcctggat gcccggatac tgttactcgg
    23161 gctgctgggt tatctgcttt ccgttttttg ttggtataaa acgctacata aacttgcgct
    23221 aagcaaagcc tatgccttgc tcagtatgag ttatgtgctg gtatggattg cttcaatggt
    23281 acttcccggc tgggaaggga ctttttcgct gaaagcacta ctgggagtag cctgtattat
    23341 gagcgggttg atgctgattt tcctgcccac gacaaaacaa cgttactgag ttttccctgc
    23401 cgctgtacac tgccgttccc actcatcggg gctataactg cttgccgaga ggactttcac
    23461 cgtatgcagc ttttcacgat taatacaata aagcgcattc tgcaaaggag agagtaaatc
    23521 ccccactttc acggcgaagt cactcttcac ttcggcgatc atttttatcg cgccgccagc
    23581 atcgcagagc ataagtacat gtctgttatc ttgtttattg cagcacgatt ttttgaccag
    23641 ccattccatt gcattatcct gtttgctaag agttttcaca tcaatttaca gattaacaga
    23701 taaactcttt atcaacggca atggaatggc agaaatgaga agtgaaacgg agggttaatt
    23761 attgctgacg ttgcacccac tcttttagcg cctgacgtga aattttaata ccgccgtttt
    23821 tcagctccgg cggtaaagtc agccagcgca ccggttgttg aaaacgagcc agcttatctt
    23881 tcacccattc gccaagatca acggtttgct ggtcatactc caccaccgct accggccgat
    23941 gaccaaactc cttgtcggca acgggaacga taaacacctg caaaaccgca ggatgtgcag
    24001 caataacacg ctcgacttct tccggctgaa taccctcacc gccactgaat aacagattgt
    24061 ctaaacgtcc gacaatggtc agcttgccat tatgcatctc accgcgatcg cgcgtagcgt
    24121 accagccttc gtcattaacc agtgaaacca gttgcccgtt acgccagtaa ccttctgcca
    24181 tactggcagc ccgcagccac acttcattat taacgatttt cacttcccga cccggcagcg
    24241 gcgaaccaac atctgccagg ccgtcggctt ctttcgcaca taccgtggag gcaaactcgg
    24301 tcagaccata gccgcaaaag caacgaatcc cctgctcgcg cgcctgttcc gtcaactcga
    24361 ccgggatagc cgcgccgcca agtaacaccg ctttcaggga aacggaactg cggttaacca
    24421 gcaaacgcca gagttgtgtt ggcaccagtg aagcgtgagt acagcctgcc agcatttgct
    24481 ccaatggctg tttatcacgc accgtcatcc gcgcaccagc gtataaccag cgccacataa
    24541 ttccctgacc ggagacgtga aacagcggta aagagagcaa ccagtcatcg tgatcgccaa
    24601 acggaatcag cgataacaca ccttgcgcac tggcaagatg ggcctgataa gtatggacag
    24661 cggctttcgg taaaccggtg gagccggagg tcaatgtcat tgaacaaaga cgtgttggtt
    24721 gccacagagc tgaatgtgtg ccttcaacca tctgaatatg cagtgaagtt aacgccggga
    24781 aagtattttc tccttccagc accagcgcat attgcagtgt aagatttggc agtaattcct
    24841 caagcaacgg ttgcggaagc tgtggattta cgggcagcac ccgcgccccg cattgcagta
    24901 acgccagcca ggcgagcagc gtttgcggcg tattccacgc ccgcaacatc acgccgctgc
    24961 cctcaaccac accctgcgcc gcaaagcccg tagctaactc atcgacgcga gcacaaagct
    25021 cgcgccagtt gagttgctcg tcattaagac gtaaggcgat ggcttctccc cgcacttgcc
    25081 gccagtgacg ccacggccag tcagagaaga tcataacaac cgctccagtg catcaacttc
    25141 cacgacaggc agcgtgctac ccggccagcg acgtacctgc tgcgcctgca tcagatccag
    25201 cgtgtccagc cctggaatgg tgtccggcgt taaccaggcg gcaatccgcg ccagttgcgt
    25261 taagcctaag ctcgactcaa tagaagaact gatcaccgcc gtcagcctca acgcgtgcgc
    25321 cgcctgtacc tgctcgcgta ctttttccag actgcccgtg agcgtgggtt tgataactac
    25381 cgcgcgcacg ccctcttcag ccacaaaggc aaaatccggc tcgcgcaggc tttcatccca
    25441 ggcaatggca atgccggttt cacgggcaaa cgctcgcgaa tcatcgcggg ttttgcacgg
    25501 ctcttcgaga aacgcgatgc ggtggcgata atccgggtta acatatttgg caaactgctg
    25561 acctttcagc ggtgtccagg cgcgatttgc gtcaagacgc aaatgcagat ccggaattgc
    25621 ctccagcaac agattaacca ccatgccgtc gcgcacggct tcgtacaatc ccactttgac
    25681 cttcgccact ttctcgcctg gcatatcagc aagtttgagg atcagatcgt caggatcgcc
    25741 attacacagt ggtgccgcac ggtaattggc cgcctgcggg agcgtatctg ccagctccgc
    25801 caatgcacag cttacgccaa aggcaacgga aggcatctgc ggtagctcgc aatcgcctgc
    25861 cagccagtta tttacccagg caagcagcac gctttgcgcc tcttcccagg tttcctggct
    25921 gaagcccggc agtggggaga tctcccccca cccttcgcgc tcgccctcac gcaggcaaac
    25981 gtacagcccg tcgcgggttt ttaaccgcct gtcgcgcaga accacccccg cgtccatggg
    26041 gatctgccag cggtatacct gcgcgctacg cattacggat tccgtttaaa tttgctgaaa
    26101 tcaggctgac gtttctggtt gaaagcgttg cgaccttcct gaccttcttc cgtcatgtag
    26161 aacagcatgg tggcgttgcc cgccaactcc tgcagccccg cctgcccgtc acagtcggcg
    26221 ttcagtgcag ctttcaggca gcgcagcgcc atcggactgt tttgcagcat ttcgcggcac
    26281 cagcgaacgg tctctttttc cagatccgcc agcggtacca cggtgttgac aaggcccata
    26341 tccagcgcct gtttcgcgtc gtactgacgg cacaggaacc agatttcccg cgcttttttc
    26401 tgtccgacga tgcgagccat gtaggaagcg ccccagccgc cgtcgaagga accgactttc
    26461 gggccagtct gaccgaagat ggcattatct gccgcgatag tcaggtcgca catcatgtgc
    26521 agaacgtgac cgccgccgat ggagtagcca gccaccatcg cgacaaccgg tttcggacag
    26581 gtacggatct gacgctggaa gtccagcacg ttcaggtgat gtacgccgga atcatcttta
    26641 tagccgccgt aatcaccacg cactttctgg tcaccgccgg agcagaacgc tttatcacct
    26701 gcgccagtca gaatgatcac gccgacgttg tcgtcataac gcgcatctgc cagtgcctgg
    26761 atcatctctt ttaccgtcag aggacggaag gcattgcgca cctgcggacg attaatggtg
    26821 atttttgcga taccatcggt ggatttttca taacgaatgt cctcgaaacc ttcggagcag
    26881 tcgtgccatt caaccggtgc gtaaagcatt gcttcatcag gataaatcat attgtgtcct
    26941 ttagtcagaa acgcaagatc tgcgccagac ttgcgattac gccagcggga ttttcccgat
    27001 gcgcgttatg tccggcgcga ggaatgacat ggcagtcggc agccagttcc gccgccaggg
    27061 cgcggaattt gctgtcacgt tcaccacata aataataaaa cgcaaatgtg cgggcgctaa
    27121 ggttagcacg taaatcaggc tggacggcga gagaagtcgc ctccagcatg gcggcaaggg
    27181 ttgcgccatt attgttgctg cgcagcgcca ccagctcccg gcgttgatcg tcattgagcg
    27241 aggcaaaaac aggctgttga taccagtcgg caaataccgc cgttaacggt tctgtgcgaa
    27301 aacgctgcgc ccattggcga tcggaacgct gacgttccgc acgttgttca gcattttgca
    27361 gccccggatg cccgccttcg acaataaccc cacaaagccc cgccagcccc tggcaagcca
    27421 ccatcatcgc cacccgtccg ccaagcgagt accccaccag ccagaagtca aggatgttgt
    27481 aactaaccaa ggttttacgc agtaagtcgg tgacatcatc aaatccatcg acgctaatcg
    27541 ccgccgaacc accatgacct gggagatcaa catacaaccg tgagtagtcg gcaaacgcct
    27601 cgcccacttc ttgccattcg tggcaatcgc cggaaaaacc gtgcaaaaac accagccagg
    27661 gtaaacctgg ttttccgtgt tttgcctgcg cgtgcaggat cataaatggc ttacctgcgc
    27721 cagaagttgc tggagcgttt gcgcaccatc ggtgtcgtta accaccattt caatcaccgt
    27781 ggtggttggc gtgcgccagg cgtcggcaaa tgccgtttca agttcctgcc agttttgcgg
    27841 acgatgatat ttcagctcga acatcgcggc ggcgtgctca aaatggacgt tttgcggcat
    27901 cagatagaaa cgctcacgct cgctttgcgg cgttggcaac agcgagaaaa tttgcccgcc
    27961 gttgttgttc accacaatta ataccagcgg cgcagaaacc tgacgcaata acgccagcgc
    28021 gttgagatcg taaagtgcgg agagatcgcc cacaatcgcc agcgtcggtt tgccgcttgc
    28081 ccgctgaacg cctgcggtgg tcgaaagcag tccgtcgata ccgctggccc ctcggttgct
    28141 gtacaccggg taacctgccg gaagttgcga aagcgcatca atcagacgta ccaccaggct
    28201 gttaccgaca aacaattgcc cctgttcagg cagatagtcg ctgatgcgat gcgccagttg
    28261 cgcttcgcca aacgcatcgc gacgggcaat aaccgcctgc attgcctgtt ccgccaggcg
    28321 cgggatttca acgcaccagg gctggcgttt ttctgccgga tgcagctcca gccagtcggc
    28381 aatattggca attaagcggc gtccacgatg gtgcgccgga tcaagccgcc cttcgatgtc
    28441 atccacaatc cagtactctt cgggttcaca gcttgcctgc cattgcagga gccgtttgcc
    28501 cgtcaggctg cttcccagtt gcaccacaat ttgcgcctgc tgcagctcgc tggtcgcttt
    28561 ggcattgcct aaccaaagat cggcacacgg cagcggctgc ccggtttgtg acagcacatc
    28621 gccaatcagc ggccagccaa gagtttgcgc ccacagggca acttttttgc cctcttccgc
    28681 actcatgcgc ccggcaacca ccacgccgcg cttttgtcgc cagaagaacc agtcgcgctg
    28741 tttttcactt tccagactag gcgcttcacg cagccacggt ttgtcgtcct gccaccagtc
    28801 acccagacgc tgttgccagc taagcccggt atcgtccatt tcgccataca gcggttcagc
    28861 aaacgggcag ttgatatgga ctccccccgc atgaagcgta ccgagagcgt ggtcgatggt
    28921 agaaaccagc caacgtgcgg ggatatcctg ggtcgggcgc ggcaatgaaa tactgtgcgt
    28981 ggggtgagag gcgaacattc ccggctggcg aattgcctga ttcgcgccgc agtcaattag
    29041 ctccggcggg cgatcggcgg ttaagagaat cagtttttct ccggttaacc cggcttcaat
    29101 cagtgccgga tagagatttg ccaccgccgt gccggaggtc acaatcaccg ccaccggctg
    29161 cttgctgact ttcgccagcc ccagcgccag atgccccaac ccacgctcat cgaaatgggt
    29221 gtggtgaatg aatgcggaat tctccgccgc cgctaacgtt aacggtgtag aacgcgagcc
    29281 tggggcgata cagatgtgtc tgacgccgtg acgcgttaat gcttccagaa tgaccgccgc
    29341 ccagcgtcgg ttaaatgcgc ttactgacat gagtttgtcc ggtatcaata ttgcggctaa
    29401 gtataaggag ctacaaaaat agattattga tatgaatcgg taatgatgcg actcattact
    29461 attccatttg taataaagta cgcaaccctg ccgctttgtt gtcgatttcc tgccactctt
    29521 gctcggggtc ggaaccacgg acaatgcccg cgccagcata taatcgcacg acattgccgc
    29581 taatttttgc tgagcgcagg gaaacgcaga attcgctttg ttgtaatgag agatagcccg
    29641 cagaaccggc gtaccattct cgggtgaacg gttcgtgaca ggcgataaac tgtcgcgcca
    29701 gatcgcgcgg taagccagca actgctgccg ttggctgcaa ctgatgtaaa cagatcacat
    29761 catccgcttt gttgagtgaa gtccagatac agcggcgaag atgctgcact ttacgcagac
    29821 gcagtacctg cggcggtaaa acatccagcg tctgggtatc ggcctgtaat cgttggcaga
    29881 tatcttccac caccagcatg ttctcgcgct ggtttttatc atccgccatc agccactctt
    29941 ctaactgctg cgcctgctta tcatcaggat gatttgctac tgttcccgcc agcgcttcag
    30001 tacgcagcgc tttgtcacgc cgccgccata accgttccgg tgaagagcca agaaaagcat
    30061 tttcgccatc aaaggccatg taaaaatggt agcaattcag attcagtcga cgactggcag
    30121 ccatcatcgc cgccgcgttg accggacttg cgaaatgcag gtcagttgcc cgagcgagca
    30181 ccactttgtc gagctcacct tcggcgatgg ttttcgttgc cagttcgatt aattgcgtcc
    30241 agcccgtttt gtccggccag tgttgttctc gcgtggtggt taaatgtaac ccaggcaagg
    30301 gcttgatact caccagtgtg gcgataaatt cttttgcctg aatcgcatcg tgctgaaggg
    30361 agctttcgct gaatagcgtc agccgcagcg tggctttacc gccacagcgt cgccattcca
    30421 ggcggggtaa aagtaaattg ccctgcgacg ggtcaaaagc attcagcccc caaatgcgta
    30481 agtcggcgtg ttccgggtgc tggcgaagaa aacgttgtgc ctggtccaac gacgtaaaac
    30541 gggtaatcgc gcccaggacg gcagcttctt catcaccatt acgttgttgc cagtagaatt
    30601 gcgggtacgt ttgctgactg gccagccagc tcaaggcatc aaaagcgtct ttgagaggga
    30661 aaggaatatc gataacccga atgccgggtg tcgccggaat ctcttgcgac aaatggcgca
    30721 gtagattttc cagcgccgta gtaagtgatt gcacgcggac ctctccctgt taaaagcctc
    30781 acattatacg gggtactaca aaaaaaatgc agtaccccgg tgtagggagg ttttaacggc
    30841 gtgccagcaa cagtcctagt accagcccaa cggccgcgcc cacaccaatt ccttgccaag
    30901 gtttttcgtg gacgtagtca tcagcacgat aaacagcctg cttcgcccga tagtaataac
    30961 tgtctgaaac ctggctaacc cgttttttca catcatccag cgctttttct gcacgcgctt
    31021 tcagctcaac atatttctga tcagcgggat cgccagagga gcggagcacc tcttccagtg
    31081 tttcactaag cagcgtcagg tcgtcatcga tacgtgtatc accaaactga ttagacattc
    31141 tcgttctcca tttgcgtaaa acctgtcact aactatagac aagggttgtt gattacgcct
    31201 gaattacctc gcgtgccatc ccaatgtgtg ggataccatc ttcttcatag acctccgtca
    31261 cagggataaa accgaaactc tggtagaagt tttgcagatg cgcctgcgcc ccgaggtaaa
    31321 caggcttatc aggccagtga tgcgtgcagg tgtcgagggt tttactcatc agttgctgcc
    31381 ccactttttc gccacgcaac gcttcactga caatcacccg accaataacg accggctcaa
    31441 gatcatcatc acttttcaga atcctcgcat atgccaccag ttcatcattt ttccaaccga
    31501 gaatatggcg attatccccc gtcaaatcat caccatcgat atcctgataa gggcaattct
    31561 gctcgacaac aaacaccgcg caacgcagtt gtaataaggc ataaagctgg gaaaccgata
    31621 attcagagtg atgcagatct tgccattcaa tcataatgct ctccttttag atgtatgcct
    31681 tatactagcc agaatatttt ttgaaacgtg atgaactcat ggaattaatt tttttaggta
    31741 cttcagccgg tgtgccaacc cgcacgcgca atgtcacggc aatattgctt aacctgcaac
    31801 atccgaccca gagcggactt tggttgtttg actgcggtga aggcacccag catcagctac
    31861 tgcataccgc ctttaaccct ggaaaactgg acaagatttt tatcagtcac cttcatggcg
    31921 atcatctttt tggtttaccc ggcttgctgt gcagtcgttc tatgtcaggc attatccaac
    31981 ccttaacgat ttatggtccg caaggtatcc gtgaatttgt ggaaaccgcg ctgcggatta
    32041 gcggctcatg gaccgattat ccgctggaaa ttgtcgaaat tggcgctggc gaaattctcg
    32101 atgatggcct gcgcaaagta accgcttatc cgctggaaca cccactggaa tgttatggct
    32161 atcgtattga agaacatgat aaaccgggtg cattaaatgc ccaggcatta aaagctgctg
    32221 gcgtgccgcc tggcccactg tttcaggaat taaaagcggg caaaacaatc acgctggaag
    32281 atggaaggca gattaacggc gcagattact tagctgctcc agtgccaggt aaagcgctcg
    32341 ctattttcgg cgataccggc ccctgcgatg ccgcacttga cctggctaaa ggtgtcgatg
    32401 tcatggtgca cgaagcgacg ctggatataa ccatggaagc caaagccaat agtcgcggcc
    32461 atagctctac acgccaggct gcgacactag cccgtgaggc tggagtcggt aagctaatca
    32521 ttacccacgt cagctcgcgc tatgatgaca aaggttgtca gcacctgtta cgtgaatgca
    32581 ggtcaatttt cccggcgact gaactagcga atgatttcac cgtgtttaac gtttaactct
    32641 gcttattatt aaaaacaggg cgaaacttgc cctgttatcg caacccgcgc tacatatatc
    32701 cacccacacg ggcatattga gcgatttttg atatttattc atctccttac gcactatatt
    32761 cagagcaata agttgcctct ttttatcttc attcagcaca acctttgcaa ctaaaaggag
    32821 cccatgatgg ttacagttgt cagcaattat tgtcaattat ctcaaaccca actcagtcag
    32881 acatttgcag aaaaatttac tgtgaccgag gaattactgc agtctttaaa aaaaacagcg
    32941 ttatccggag atgaagaaag tattgagtta ctgcataata ttgcgttagg ttatgatgaa
    33001 tttggaaaaa aagctgaaga tattctttac catattgtta gaaacccaac aaatgagacc
    33061 ctatcgatta tccgacttat taaaaatgcc tgtttaaaat tatataattt ggcacatacc
    33121 gcaaccaact tccaccttaa accaactggt ccggataatt cagatgttct cctgttcaaa
    33181 aagctattct ccccttcgaa attaatgaca attatcggtg atgaaattcc tcttatatct
    33241 gaaaaacagt cgctttcaaa ggtgctttta aatgatgaga ataatgaact gagtgatggt
    33301 acaaacttct gggataaaaa tcgtcaatta accacagatg aaatagcttg ctatcttcag
    33361 aagatcgccg ctaatgcaaa aaatactcaa gtcaattatc ttactggtct ctacgtcccc
    33421 tactccacca gaactcacct ggaagacgct ctcaatgaaa atattaagag cgatccatca
    33481 tggccgaatg aagtccagtt attccccata aatactggcg gacactggat attagtttcg
    33541 ctacagaaaa tagtaaataa aaaaataata aactacaaat aaaatgtgtc atattcaact
    33601 cattgcgtgc actaggccat gataaagaaa attcacttaa gcgtgtcatt aatagtttta
    33661 attctgaact catgggagaa atgtcgaata ataatataaa agttcattta aatgaaccag
    33721 agataatatt tttacatgcc gatcttcagc aatacttaag ccaaagttgc ggtgcgtttg
    33781 tgtgcatggc agcccaagaa gtgattgaac aaagggaaag caattctgac agcgccccct
    33841 atacgttatt aaaaaactat gctgacagat ttaaaaaata ttcagcagaa gagcagtaca
    33901 aaattgattt tcaacatcga ctggtaaaca ggaattgtta tttagataaa tatggcg
//
LOCUS       DAJTNA010000021        33852 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTNA010000021 DAJTNA010000000
VERSION     DAJTNA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33852
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.021"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..369
                     /locus_tag="OO161_000875"
     CDS             <1..369
                     /locus_tag="OO161_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5462039.1"
                     /translation="RRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGINPT
                     IIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIRN
                     HRYTATMLQHIAEDRDGTNH"
     gene            complement(366..1843)
                     /gene="scpC"
                     /locus_tag="OO161_000876"
                     /pseudo
     CDS             complement(366..1843)
                     /gene="scpC"
                     /locus_tag="OO161_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(1867..2652)
                     /gene="scpB"
                     /locus_tag="OO161_000877"
     CDS             complement(1867..2652)
                     /gene="scpB"
                     /locus_tag="OO161_000877"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5462040.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(2663..3658)
                     /gene="meaB"
                     /locus_tag="OO161_000878"
     CDS             complement(2663..3658)
                     /gene="meaB"
                     /locus_tag="OO161_000878"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5462041.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(3651..5795)
                     /gene="scpA"
                     /locus_tag="OO161_000879"
                     /pseudo
     CDS             complement(3651..5795)
                     /gene="scpA"
                     /locus_tag="OO161_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(5999..6892)
                     /gene="argP"
                     /locus_tag="OO161_000880"
     CDS             complement(5999..6892)
                     /gene="argP"
                     /locus_tag="OO161_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5462042.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSLIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7275..7934
                     /gene="rpiA"
                     /locus_tag="OO161_000881"
     CDS             7275..7934
                     /gene="rpiA"
                     /locus_tag="OO161_000881"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5462043.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8189..9421
                     /gene="serA"
                     /locus_tag="OO161_000882"
     CDS             8189..9421
                     /gene="serA"
                     /locus_tag="OO161_000882"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5462044.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            9650..9709
                     /gene="ibsC"
                     /locus_tag="OO161_000883"
     CDS             9650..9709
                     /gene="ibsC"
                     /locus_tag="OO161_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5462045.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10042..10101
                     /locus_tag="OO161_000884"
     CDS             10042..10101
                     /locus_tag="OO161_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5462046.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10202..10804)
                     /locus_tag="OO161_000885"
     CDS             complement(10202..10804)
                     /locus_tag="OO161_000885"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5462047.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(10825..11008)
                     /gene="ssrS"
                     /locus_tag="OO161_000886"
     ncRNA           complement(10825..11008)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO161_000886"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11050..11379)
                     /gene="zapA"
                     /locus_tag="OO161_000887"
     CDS             complement(11050..11379)
                     /gene="zapA"
                     /locus_tag="OO161_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5462048.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            11547..12125
                     /gene="ygfB"
                     /locus_tag="OO161_000888"
     CDS             11547..12125
                     /gene="ygfB"
                     /locus_tag="OO161_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5462049.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12151..13476
                     /gene="pepP"
                     /locus_tag="OO161_000889"
     CDS             12151..13476
                     /gene="pepP"
                     /locus_tag="OO161_000889"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5462050.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            13473..14651
                     /gene="ubiH"
                     /locus_tag="OO161_000890"
                     /gene_synonym="visB"
     CDS             13473..14651
                     /gene="ubiH"
                     /locus_tag="OO161_000890"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5462051.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            14674..15876
                     /gene="ubiI"
                     /locus_tag="OO161_000891"
                     /gene_synonym="visC"
     CDS             14674..15876
                     /gene="ubiI"
                     /locus_tag="OO161_000891"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5462052.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(15950..16033)
                     /locus_tag="OO161_000892"
     CDS             complement(15950..16033)
                     /locus_tag="OO161_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462053.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16323..17417
                     /gene="gcvT"
                     /locus_tag="OO161_000893"
     CDS             16323..17417
                     /gene="gcvT"
                     /locus_tag="OO161_000893"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5462054.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17441..17830
                     /gene="gcvH"
                     /locus_tag="OO161_000894"
     CDS             17441..17830
                     /gene="gcvH"
                     /locus_tag="OO161_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5462055.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            17949..20822
                     /gene="gcvP"
                     /locus_tag="OO161_000895"
     CDS             17949..20822
                     /gene="gcvP"
                     /locus_tag="OO161_000895"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5462056.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21088..21831
                     /locus_tag="OO161_000896"
     CDS             21088..21831
                     /locus_tag="OO161_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5462057.1"
                     /translation="MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGELGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(21888..23321)
                     /gene="bglA"
                     /locus_tag="OO161_000897"
     CDS             complement(21888..23321)
                     /gene="bglA"
                     /locus_tag="OO161_000897"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5462058.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23366..23677
                     /locus_tag="OO161_000898"
     CDS             23366..23677
                     /locus_tag="OO161_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR5462059.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            23841..24500
                     /locus_tag="OO161_000899"
     CDS             23841..24500
                     /locus_tag="OO161_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5462060.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(24577..25557)
                     /gene="ygfZ"
                     /locus_tag="OO161_000900"
     CDS             complement(24577..25557)
                     /gene="ygfZ"
                     /locus_tag="OO161_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5462061.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            25800..26066
                     /gene="sdhE"
                     /locus_tag="OO161_000901"
     CDS             25800..26066
                     /gene="sdhE"
                     /locus_tag="OO161_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5462062.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26047..26454
                     /locus_tag="OO161_000902"
     CDS             26047..26454
                     /locus_tag="OO161_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR5462063.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(26494..27015)
                     /gene="fldB"
                     /locus_tag="OO161_000903"
     CDS             complement(26494..27015)
                     /gene="fldB"
                     /locus_tag="OO161_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5462064.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27127..28023
                     /gene="xerD"
                     /locus_tag="OO161_000904"
     CDS             27127..28023
                     /gene="xerD"
                     /locus_tag="OO161_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5462065.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28048..28758
                     /gene="dsbC"
                     /locus_tag="OO161_000905"
     CDS             28048..28758
                     /gene="dsbC"
                     /locus_tag="OO161_000905"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5462066.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            28764..30497
                     /gene="recJ"
                     /locus_tag="OO161_000906"
     CDS             28764..30497
                     /gene="recJ"
                     /locus_tag="OO161_000906"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5462067.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            30588..31686
                     /gene="prfB"
                     /locus_tag="OO161_000907"
     CDS             join(30588..30662,30664..31686)
                     /gene="prfB"
                     /locus_tag="OO161_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5462068.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            31696..33213
                     /gene="lysS"
                     /locus_tag="OO161_000908"
     CDS             31696..33213
                     /gene="lysS"
                     /locus_tag="OO161_000908"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5462069.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33256..33804)
                     /gene="idi"
                     /locus_tag="OO161_000909"
     CDS             complement(33256..33804)
                     /gene="idi"
                     /locus_tag="OO161_000909"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5462070.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
BASE COUNT         7978 a         8582 c         9156 g         8136 t
ORIGIN      
        1 cgccgttatg aagagcgtca agagcaagag ctgcttcagg agctgccctg gctacaaatg
       61 cgctttcaaa accgggctaa cttcgaagca atgattgatg cgaatatgcg accttgtggg
      121 atcaatccca ccattattta tcgtccctac agttttatgg ccaaaatcag cgccgtggag
      181 cgcggacatt ttctgacggt gatcccgcat tttgcctggc gactggtgaa tccggcaacg
      241 ctgaaatatt ttgatgctcc gcacaggccg atgtatatgc aggaatatct ttattcaatc
      301 agaaatcatc gttataccgc cacgatgctt cagcatattg ctgaagatcg tgacgggaca
      361 aaccattaac cctgcatcga gccggttgca attaaattac ggtgtaagtc gaagacgtgg
      421 ctaagatcgt ggtgaatatg tccgccgggc gcattttcca gatagcgatg cagataatcc
      481 cgatacatag gatgtgcaca attatcaatg atagtgcggg cgcgttgaag cggggaaaga
      541 ccgcgcagat cggcgatccc ttgttcggtg atgatcactt tgacgctgtg ttcgctgtga
      601 tcaacatggc tgcacattgg cacgacggtt gagatcttgc cttctttagc aatcgacgga
      661 gccataaaga tcgacagata cgcgttgcgt tcaaaatcac cgctgccacc gatgccgttc
      721 atcagatcga ccccggctac gtgcgttgaa tttgcatgcc cgtaaatatc aaactccagg
      781 ccgacgttca gagcgatgac gcccagacga cggatgattt ccgggttatt ggaaatctcc
      841 tgcggacgca acacaatgcg gctggcaaag taatccatat tgtcgtaaat cttgcgcagg
      901 gaatcggccg agattgtcag gctggaggcg ctggccccgc tgattttgtc ggtttccagt
      961 aaatgcacca ccgattcctg tagcacttcc gaatacatca tgaacggagg aatttccggg
     1021 ttttccccca gacgcgccat taccgcatta ttgatattgc ccacgccact ttgcagcggc
     1081 agaaattccg gcggaatacg cccatgcgcc atttcctgca ataagaacgt gaccacgtta
     1141 tcggcaatct gctggcacat gggattttgc ttatccagca tattaccggc gtcgggcaag
     1201 ttggtttcca cgacggcgac aatctttttc ggatcgattt gcacatagcg ggtaccgacg
     1261 cgatccattg catggaagat cgacacgcta ttgcgccgcg gtggcgcgcc aggaatcaca
     1321 atatctgcca gtactgcaac gcgcggatcg tgatagtgat taagttcaat gatcactttc
     1381 ttcgctcgca gcagccaggt cggcgcatta ccgatcccgc tggttaacca gactcgacca
     1441 tccggtgcca gtgccgatgc ttcaatgacg gcaacatcaa tgtcgccgaa gaaaccgtaa
     1501 ttgaccattt gcgccacttc gctcaaatgc aggtcaacga aactcaccgc gccctgattg
     1561 atctttttac gtaaaccgga cgatgttgat atggcgcacg ccaggaaaca gcatcggcgt
     1621 cagaaagtac atcgtcagcg gcggcgctga ttgacgcccc cgtcagcagg cgaatttgat
     1681 acggcttttt ggcctcatgc tgttcgttag ctctgcgggc aatcgcggtg ggtagggctt
     1741 tcggcgaacc cgccggggta aagctgctaa atgccaccat gtcgttatgc tggataattt
     1801 ctgccgcttc atcggcggtc atccttgtcc actgagtttc catttttact ccttcgttcg
     1861 cagggattaa tgaccaacga aattaggttt acgtttttcg aggaaagcgt tcattccttc
     1921 ctggtaatct tcgctgtcat aaaccgcgcg tcgcatcccc tgaatacgtt caaattcatc
     1981 ggagttcatg gtgtgcgctt cgcccagtac acgcagctct tctttgataa cagcaatggc
     2041 taacggcgct ttctcagaga tgtggtgcgc catttgtaag gtgaaatctt ccagttcttc
     2101 cacttccaca acatggttga ggatgccgac agccagcgcg cgctgggcgg tgattggcga
     2161 agcggtaaaa atcagctctt tgacaatgtg gaagcccgcg tcgcgggtca ggttgtgaat
     2221 gccgaccagg ttatacggga cgccgaggtt tacaggcgtc attgagaagg ttgaggtact
     2281 ggcggcgatg atcagatcgg aactcatgat catttcaaat gcgccacccc aaacactacc
     2341 ttccaccatc gaaatgatcg gtttcgggaa tttttggatc atgcgggtga tttgacgcaa
     2401 tggatcatca taggagagcg gatcgcgacc gccagacggc agttcgtgaa tatcgtgacc
     2461 tgcggagaag actttggatc cactcggcgc gcgcaaaatg atacagcgaa tttccggccg
     2521 gttgagatcg cttaacgcct gcataagatc atcaataaag actttactta aggcattaag
     2581 ttttcggcca tagttaaact caatgaccgc cactttgttg atagtggcaa cgttcacata
     2641 ctgataagac ataaaaattc ctttaatcaa aatattgcgt ctggataaat tcactgagct
     2701 gccgcaggcc ggtgcgcggt gagagcgtat tgtttttgac cgctaaaagc gtctggcggt
     2761 aatagcgatc gaaatcttca ttcgcgaaca ggtgattcag tacttcttct tcggtctgct
     2821 tacgcagcca ttccaccgat tgttgttgcc gcacttgttg taaacgacca ctggcagtta
     2881 gcgcggtttt gaactcgatg atggcgtgcc agatctcatc gattccacgt ttttccagtg
     2941 cgctacaagt cagaacccgt ggctgccatt cgtcgtattt acgtcgcaga atatgcaggg
     3001 cactctcgta catatgccgg gcaatggcga cattggtatg gttatcgcca tcgtctttgt
     3061 tgataacgat cagatcagcc acttccatca gccctttttt aatgccctgc agatcatcgc
     3121 cgccaccggc aatttgcaac gagataaaac agtccaccat gcgggcgact tctgtttccg
     3181 actgcccgac gccaaccgtt tcgacaatca ctacgtcata acccgccgct tcgcataaca
     3241 gcattaattc ccgcgctcgc tgactggcac cgcccagatg accggaggat ggtaccgggc
     3301 gaataaacgc cgcttcggca cgcgccaggt cattcatgcg gattttatcc ccgagaatgc
     3361 taccgccagt gaccgggctg ctgggatcga ccgcaataac cgcgaccttt aatccctctc
     3421 gaatcaacaa catgccaaag gcctcaagaa aggtactttt ccccgcgctg ggggtgccgg
     3481 taacgcccag tcgcagggtg ttaccgcagt acggcataat ggcatcaagc agctgcgtac
     3541 ttagtgcctg atgacgcggg tgacggcttt ccaccagcgt catggcctgg gcgagtgtgg
     3601 cacgctcacc ctgacgtaag cggcgaatac tttctgccag cgtggcttca ttaatcatga
     3661 tgctggctta tcagattcag tacgtcgcgc acactgtcga gcataggtgt acctggacca
     3721 taaatcgccg ccacgccgcg ctcttgcagg aaggcgtaat cctgcggtgg aatgacgcca
     3781 ccggcgacta cgcagatatc ttcgcgtccc cattttttca gcgcctcgac cagttccggg
     3841 atcagcgttt tatgaccggc agccagtgag gatgggccca ctacgtgaac gtcattttct
     3901 actgccaggc gggcgatctc ttcaggtgta gagaacatcg ggcttaaatc tacgtcgaaa
     3961 ccgagatcgg aataggcgct ggcgatcact ttcgagccgc gatcgtgccc atcctggccc
     4021 attttggcga tcagaatgcg cgggcgacga ccattgtcgg caaggaactg ctccgtttgc
     4081 gcaacaatgg catcgaactc ggaggccgat ttctcagatt gatgatagct ttgcgcaatc
     4141 acgccggtaa cacactggct tggcaccaga taacggtcga aagcggcttc cagcgcatcg
     4201 gaaatttcac ccagggtggc gcgaacgcga gcggcattaa cagcggcagc cagcaggttt
     4261 tcgttatgct gtgcggcgtg agtcagggcg ttcaacgcgg ctgttacggc ggcatcatca
     4321 cgggtggcgc gaatgcgttc cagcgaagca atttgctcgt tacgcaccat cacgttgtcg
     4381 atctcaagta catcggtttc gtcttcgtga tccagtttgt acttgttgac accaacgatg
     4441 acacgtttgc cctggtcgat aagcgactgt tcgcgcgctg aggcctattc gatcattcgt
     4501 tttggcagac ctgcttcgat cgctttcgcc ataccaccgg cttcgtcgat ctgttggata
     4561 atagctctgg cttgtttgac gatttgatcg gtcagcgact caatgtaata ggatccggcc
     4621 agtggatcga cggtgcggca gagttctgat tcttcctgga tgatgatctg ggtgttgcgg
     4681 gcaatgcgtg ctgagaaatc ggtaggcaaa ccaagcgctt cgtcaaaggc gttggtatgc
     4741 agtgactgag taccgcccag cgtcgcagcc agtgcttcaa tggtggtgcg gataacgttg
     4801 ttatacggat cctgttcagt caggctccag cctgaggtct ggcagtgggt acgcagcgcc
     4861 agtgatttcg ggtcctgtgc gccaaatcca ctgaccgctt cgctccataa ataacgtgcc
     4921 gcacgcaaca tggcgacgtt cataaacaga tccatgccga tgccgaagaa gaacgacagg
     4981 cgaggagcga agtcatcaat tttcagtccg gcagagattg ctgctttgat gtactcaatc
     5041 ccatcagcga gcgtaaatgc tacctgctgc acgcagttgg cacccgcttc acccatgtgg
     5101 taaccgctga tactgatggt attaaatcgc ggcatgttgc cggaacacca ggcgatgatg
     5161 tcggcgataa tgcgcattga cggttttggc gggtaaatat aggtgttgcg gcagaggtac
     5221 tctttgagga tatcgttctg aatggtgccg gtcagtttat caggtgtaac accttgctct
     5281 tccgcggcga cgatataaaa cgccagtact ggtagcactg cgccattcat ggtcatcgaa
     5341 accgacattt tatccagcgg gatctggtcg aacaggactt tcatatcttc cacggtgtcg
     5401 atagcgacgc ccgctttgcc gacgtcgccc gccacgcgcg ggttatcgga gtcgtagcct
     5461 cggtgggtgg caaggtcaaa cgcaacggaa agaccttttt gcccggcggc caggttacgg
     5521 cgataaaaag cgttggactc ttttgctgtt gaaaaaccag catactgacg gatggtccac
     5581 ggttgggcgg tatacatagt ggcacgcggg ccacgaacgt agggcggcaa accaggaagg
     5641 gtacctgtca cctccagatt atcgagatcg gcttcggtat acagcggctt gatggtgatc
     5701 ccttccgcgg tttgctgaac cagcgagtcg acagttttct cccgacggct caattccttg
     5761 ttggcaagct gttgccactc ctgcacgtta gacattattt gctccgtaaa acgcaatcaa
     5821 aatgagggta tttgcgccta cggtgagctt tttacgtggg tttgtcgcca gcaaaaaaaa
     5881 tgcattcaga cgtcgttttt tgccgcctga gatttgtcta ttttgtgatg tgattcaagg
     5941 aatacaaaaa gaagtgagga caacgcctga tatgtgctat caggcattat ttgatggatt
     6001 aatcctgacg aaggactttg tgaccataat cgagcaacgc atcagtgact ttacgcatca
     6061 tgcggctttc aggagcaaag cggtgccagt agagcatccg gcgttgaaat agccctggcg
     6121 ttaagtcaat cagttcaccg ctggctagtt ctttctcgat ttgcaggtgc gggatcatac
     6181 agcaggtggt gccctggcga gcaagttgca cgaacgcttc tgaagaatta acgatatggc
     6241 agggcacgct gcctggaggc agatcgaagt tttgctgcaa aaaggcctgg tgcatatcgt
     6301 caagatggtc aaaggcgacc acaggtgctt tcaataatgc cgaacgcgtt acgccgttag
     6361 ggaaatattt ttcggcaaac ggttttgagc tgacgaacag atagtcaagc gcaccgagct
     6421 tatcgacaag gcaactcggc agcgcctgat gttgaatact caccgcgccg accacttcgc
     6481 cgcggcgcag acgttcctga gtacgggttt catcttctac ctgcaagttg aggcggataa
     6541 gcgaatcagc caatacagga gccagtgccg gaagcaacca cgtcgccaga ctgtcggcgt
     6601 tgaccgccag tgaaagcagc agcggagtcg aaccggtttg ttcatcgccc agccactctt
     6661 cttccagcaa ctccacctgg cgcagcagtg ccagcagttt ttgcccttgt tccgtcgggc
     6721 gcggcggtac ggtacgcacc aacagcggct gcccgaacat attttccagt tgcttaatgc
     6781 gctgtgagac ggctgattgt gtaatgcaca gcttttgtgc cgcgcgctca aatcctcgtt
     6841 cacgtatcac cgcatccagt gcctgtaatg ttctgtagtc cgggcgtttc attgctgttg
     6901 tctgctcctg gtaaatgctt tttcctgcac tatgacacaa ttttgtgtca ggttgcatat
     6961 accgcagccg tcggcgggta acatttgtat caatggttaa acgcgcagct taaaagatca
     7021 gggatttgcg ctattgcatt tcgcttagcg acttcgcgcc ctggttatgt tgaatggcgt
     7081 ggcgttattg cctctgttcg caccgtggag cggcataccc agaccgttgt atgctttatt
     7141 tgcggggcga aaggggatgc ccgccattgc gcgatatttt atggatgagt taaccacccg
     7201 gcttgccaac ggggtctgaa tcgctttttt tgtatataat gcgtgtgaaa tttcatacca
     7261 caggcgaaac gatcatgacg caggatgaat tgaaaaaagc agtaggatgg gcggcacttc
     7321 agtatgttca gcccggcacc attgttggtg taggtacagg ttccaccgcc gcacacttta
     7381 ttgacgcgct cggcacgatg aaaggccaga ttgaaggggc cgtttccagt tcagatgctt
     7441 ccactgaaaa actgaaaagc ctcggcattc acgtttttga tctcaacgaa gtcgacagcc
     7501 ttggcatcta cgttgatggc gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg
     7561 gcggcgcggc gctgacccgt gaaaaaatca ttgcttcggt tgcggaaaaa tttatctgta
     7621 ttgccgacgc ctccaagcag gttgatattc tgggtaaatt cccgctgcca gtagaagtta
     7681 tcccgatggc acgtagtgca gtggcgcgcc agctggtgaa actgggcggt cgtccggaat
     7741 accgtcaggg cgtggtgacc gataatggca acgtgatcct cgacgtccac ggcatggaaa
     7801 tccttgaccc gatagcgatg gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg
     7861 gcttgtttgc taaccgtggc gcggacgttg cgctgattgg cacacctgac ggtgtcaaaa
     7921 ccattgtgaa atgatctgac gggggaacct cccccgttaa aaaaattctc ttcattaaat
     7981 ttggtgacat gtgtcacgtt tttacctggc tattgtcgat tgctctaaat aaatcctcta
     8041 aaccagcata ttcaaccaag aaataccttt gcgtgatatt tcctcaacat cgcgacgcaa
     8101 acgttcatat tgccgcaata ttattttttg atatgttgaa aggcggatgc aaatccgcac
     8161 acaacatttc aaagacagga ttgggtaaat ggcaaaggta tcgctggaga aagacaagat
     8221 taagtttctg ctggtagaag gcgtgcacca aaaggcgctg gaaagccttc gtgcagctgg
     8281 ttacaccaac atcgaatttc acaaaggcgc gctggatgat gaacaattaa aagaatccat
     8341 ccgcgatgcc cacttcatcg gcctgcgatc ccgtacccat ctgactgaag acgtgatcaa
     8401 cgccgcagaa aagctggtcg ctattggctg tttctgtata ggaacaaacc aggttgatct
     8461 ggatgcggcg gcaaagcgcg ggatcccggt atttaacgca ccgttctcaa atacgcgctc
     8521 tgttgcggag ctggtgattg gcgaactgct gctgctattg cgcggcgtgc cggaagccaa
     8581 tgctaaagcg caccgtggcg tgtggaacaa actggcggcg ggttcttttg aagcgcgcgg
     8641 caaaaagctg ggtatcatcg gctacggtca tattggtacg caattgggca ttctggctga
     8701 atcgctggga atgtatgttt acttttatga tattgaaaat aaactgccgc tgggcaacgc
     8761 cactcaggta cagcatcttt ctgacctgct gaatatgagc gatgtggtga gtctgcatgt
     8821 accagagaat ccgtccacca aaaatatgat gggcgcgaaa gaaatttcac taatgaagcc
     8881 cggctcgctg ctgattaatg cttcgcgcgg tactgtggtg gatattccgg cgctgtgtga
     8941 tgcgctggcg agcaaacatc tggcgggggc ggcaatcgac gtattcccga cggaaccggc
     9001 gaccaatagc gatccattta cctctccact gtgtgaattc gacaacgtac ttctgacgcc
     9061 acacattggc ggttcgactc aggaagcgca ggagaatatc ggcctggaag ttgcgggtaa
     9121 attgatcaag tattctgaca atggctcaac gctctctgcg gtgaacttcc cggaagtctc
     9181 gctgccactg catggtggtc gtcgtctgat gcacatccac gaaaaccgtc cgggcgtgct
     9241 aactgcgctg aacaaaatct tcgccgagca gggcgtcaac atcgccgcgc aatatctaca
     9301 aacttccgcc cagatgggtt atgtagttat tgatattgaa gctgacgaag acgttgccga
     9361 aaaagcgctg caggcaatga aagctattcc gggtaccatt cgcgcccgtc tgctgtacta
     9421 attccccttc tctgaaaatt aacgggcagg tcactgactt gcccgttttt ttatcccttc
     9481 tccacaccgt tgccgcgttg cgcattctac tttgcgagtc tgctccagaa aataaatcaa
     9541 agtcagatca atgcgttgtg tttatttaat ggggatttac gatggcggga cagcatgggg
     9601 ctgtaacggt aaagccctca ccgaagcgag ggcttaaagg agaagggtta tgatgcgact
     9661 tgtcatcata ctgattgtac tgttactgat aagtttcgga gcttattaac agtcagtctc
     9721 tggggaggag tcatcctccc ttacccttac tcactaaatt aggtcaaaga atcaacgatg
     9781 tcaatcaggg cgatgcgggg tgcatcgccc tgaaccgaac tttttgatag ccacttcatc
     9841 gcccacgttt actgccgtaa tagatccttt ttcccaaaaa ttatctctgt tttgcatggg
     9901 gtttttcgaa cacgctcgaa gaaaagattt aaaggtagat caatgcgttg tggttattta
     9961 aagagagctt acgatagctg ggcagcatgg ggctgtaacg gtaaagccct caccgaagcg
    10021 agggcttgaa ggagaagggt tatgatgcga cttgccatca tactgattgt actgttactc
    10081 ataagtttcc cagcttatta acagtcagtc acaggggagg agcaatcctc ccttaccctt
    10141 actcactaaa ttaggttaaa gaatcaacga tgtcaatcag ggcgatgcag gtgtatcgcc
    10201 cttaccactc ccagactttc gacggtgtaa ccaccgcagg aagagggata tcccactctt
    10261 caacggggag tttttccacc aactgacaat catgcgcata acccaccggt tgcgttttat
    10321 agtgctgcca gttttgtaag gtccgatcat aaaaaccacc gcccattccc aggcgctgac
    10381 cgtactcatc aaaggcgacc agcggtgtga tcagcacgtc taatcgggaa aggggtagca
    10441 cgtcacgcac atccagtttt ggctcatgga ttttcaacct gttcatcacc agttcgcttt
    10501 gcggatggta attcaggaac agcaaattac cagcactaaa gggatgcaaa actggaagat
    10561 atacgcgctt accggcgcgc cagagttgtt ctattagcgg ctgggtgtcg agttcaccat
    10621 caaaagagag gaatacagcg accgtatgcg ccatcaccac cgggggataa gtcatcatcc
    10681 gggtagcggc ttgttgaccc atttcctgct gttgttccgg cgttaacgca cgacgacgtt
    10741 gccgaatcat tttgcggatt tcttgtcggg ataatgtcag tgggagttct ggtagttgcg
    10801 tcatggctgg tgccagataa gaagggaatc tccgagatgc cgccgcaggc tgtaaccctt
    10861 gaacccttgg ttcaaggtga atgtgtcgtc gcagttttaa ggcttctcgg acggaccgag
    10921 catgctcacc aaccgcggag cgccacattc ttgtggtatg aaatatcggc tcaggggact
    10981 ggcccgcttg cgaacatctc agagaaattt tgtcttcacg gttactctac cacagtaaac
    11041 cgaaaagtgt cattcaaagt tttggttagt tttttcggtg atgcgacctt gttcaagtaa
    11101 cgcttgttct atggtctgct gcagcatccg aatacgctgt tccatacttg ccgcgtagtc
    11161 acgagtcttc gctttttctt gcgctaactc atagctgata ttcaatgcgg caatgaagac
    11221 caactgttca gtatttgtga ctctagtgcg ttctttcaga tcttgcaacc gttggttcag
    11281 atcgtccgct gcctgattca acgcatccct ttggtcaggc gggcagttca cacgcagtga
    11341 acggccaaaa atttggatat cgacgggttg tgcagacatg ccaccttcct gctgattgac
    11401 tgcgctgcct tcgtctttag acccatggtc tgcgaagggg cgacactata gctaccctga
    11461 tgagaagaga caagcccttt tctggtccac cagggcccaa agtggtagca tatcatgaat
    11521 attcctccct ttgacgacga atgcttatgt ctatacagaa cgaaatgcct ggttacaacg
    11581 aaatgaacca gtatctgaac caacaaggga cgggtctgac ccctgccgag atgcatggtt
    11641 taatcagtgg gatgatatgt ggcggtaacg atgacagctc atggctgccg ctacttcacg
    11701 acctgacgaa cgaaggcatg gctttcggtc atgagctggc acaggcacta cgtaaaatgc
    11761 actctgccac cagcgatgcc ctgcaggatg acggcttcct ttttcagctt tatctgcctg
    11821 atggcgatga tgtcagcgtt ttcgatcggg ctgatgcatt ggcaggttgg gtcaatcact
    11881 tcctgcttgg tcttggcgtt acgcaaccga agctggataa agtgaccggc gaaaccggtg
    11941 aagctatcga cgatctgcgt aacattgcgc aactgggtta cgacgaagac gaagatcagg
    12001 aagagcttga aatgtcgctt gaagagatca tcgaatacgt tcgtgttgcc gcgctgttat
    12061 gccacgacac ctttactcat ccgcaaccga ccgcgccaga agtacaaaaa ccgactctac
    12121 actaaaaaca aaaaacgtaa ggagagtgtt atgagtgaga tatcccggca agagtttcag
    12181 cgtcgccgtc aggccctggt ggagcaaatg caacccggca gcgccgcgct gatttttgct
    12241 gcaccagaag taacacgtag cgccgacagc gaatacccct atcgtcagaa cagtgacttc
    12301 tggtacttca ccggctttaa cgaaccggaa gcggtgctgg tgctgattaa aagcgatgac
    12361 actcataacc acagcgttct gtttaaccgc gttcgcgacc tgacggcgga gatctggttt
    12421 ggccgtcgct taggccagga tgccgcgcca gagaaactgg gcgttgaccg cgcactggca
    12481 ttcagcgaaa tcaatcagca actttatcaa ctacttaacg gcctggatgt ggtttaccat
    12541 gcccagggcg aatatgcata tgctgatgaa atcatgaaca gtgcgctgga aaaactgcgt
    12601 aaaggttcgc ggcaaaatct caccgcaccg gcaacgatga tcgactggcg tcctgttgtt
    12661 catgaaatgc gcctgttcaa atcgccagaa gagattgccg tactccgccg cgcgggagaa
    12721 atcaccgcca tggcacatac acgggcgatg gaaaaatgcc gtccgggaat gttcgagtac
    12781 catctggaag gcgaaattca ccacgaattt aaccgccacg gtgcgcgcta tccgtcctat
    12841 aacaccattg tcggcagcgg tgaaaacggc tgcattctgc actacaccga aaacgagagt
    12901 gaactgcgcg acggtgacct ggtgttgatt gacgcgggtt gtgaatacaa aggttacgcg
    12961 ggcgatatta cccgcacctt cccggtcaac ggcaaattca cccaggccca gcgtgaaatc
    13021 tacgacattg tgctggagtc tctcgaaacc agcctgcgcc tgtatcgtcc gggaacttcc
    13081 attctggaag tcactggtga agtggtgcgc atcatggtta gcggcctggt aaaactcggc
    13141 attctgaaag gtgatgttga tgaactgatc gctcagaatg cccatcgtcc tttctttatg
    13201 catggcctta gccactggtt agggctagat gtccatgacg ttggcgttta tggtcaggat
    13261 cgctcgcgca ttctggaacc gggcatggta ctgaccgtag agccagggct gtatattgcg
    13321 ccggatgcag aagtgccaga acaatatcgc ggtatcggca ttcgtattga agacgacatt
    13381 gtgattaccg aaaccggtaa cgaaaacctc accgccagcg tggtgaaaaa gccggaagaa
    13441 atcgaagcgt tgatggctgc tgcgagaaag caatgagcgt aatcatcgtc ggtggcggca
    13501 tggcgggcgc gacgctggcg ctggctattt cccggttaag tcacggggcg ctgccggtac
    13561 atttgattga agcgactgcg ccagagtcac atgctcatcc gggctttgat ggacgagcga
    13621 tagcgctggc ggcgggtacc tgtcagcaac tggcgcgcat cggcgtctgg caatctctgg
    13681 cggattgcgc aactgccatc accaccgtgc atgtcagcga tcgtggtcac gctggatttg
    13741 tcaccctcgc cgcagaagat taccaactgg cggcgctggg acaggttgtc gaattgcaca
    13801 atgtcgggca acggctgttt gcattgctgc gtaaagcacc tggcgtaacg ctgcattgcc
    13861 ctgatcgcgt ggctaacgtt gcccgtactc agagtcacgt tgaagtgacg ctggagagtg
    13921 gcgagacgct gacgggccgc gtgctggtag cagctgatgg cacccattca gcgttagcca
    13981 ccgcgtgcgg cgttgactgg cagcaggagc cttacgaaca actggccgtg attgccaacg
    14041 ttgcgacttc cgttgcgcac gaaggccgcg cttttgaacg cttcacgcaa catggcccgc
    14101 tggcgatgtt gccaatgtct gacggacgct gttcgctggt ctggtgtcat ccactggaac
    14161 ggcgcgaaga ggtgctgtcg tggagcgacg agaagttttg ccgtgaactc cagtcggcct
    14221 ttggctggcg acttggaaaa attacccacg ccggtaaacg cagtgcttat ccgctggcgt
    14281 taacccgcgc cgccagagct attacccatc gtaccgtgct ggtgggcaat gcggcgcaaa
    14341 ctctgcaccc gattgccggg caagggttta acctcggtat gcgagatgtc atgagccttg
    14401 cggaaaccct gactcaggcg catgagcgcg gagaagacat cggtgattac ggcatattgt
    14461 gccgctatca gcagcgtcga cagagcgatc gcgaagcaac catcggcgtc acggacagcc
    14521 tggtacatct ttttgccaac cgttgggcac cgctggttgt cgggcgcaac atcggactga
    14581 tgacgatgga attattcacc ccggcacgcg atgtgctggc gcagcgcacc ctcggttggg
    14641 tggcgcgttg aaggcggttt taaggaataa acaatgcaaa gtgttgatgt agccattgtt
    14701 ggtggcggca tggtggggct ggcggttgcc tgcggcttac aggggagcgg cttacgcgtt
    14761 gccgtactgg agcagcgcgt accggaacct ctggcggcgg atgcaccacc acaactgcgc
    14821 gtttcggcta tcaatgccgc cagcgaaaaa ttactcaccc gtcttggcgt ctggcaggac
    14881 attctctccc gtagggccag ttgttatcac ggtatggaag tgtgggacaa agacagtttt
    14941 ggtcacattt cgtttgacga tcaaagcatg ggctatagcc atcttgggca tatcgttgaa
    15001 aactcagtga ttcactacgc gctgtggaac aaagcgcagc agtcgtcaga tatcactctg
    15061 ttggcccccg cagaattaca gcaggtcgcc tggggagaaa atgaaacttt cctgacgctg
    15121 aaagatggca gcatgttaac ggcgcgtctg gtgattggcg cggacggtgc taattcctgg
    15181 ttgcgcaaca aagcagatat ttcgctgact ttctgggatt atcagcatca cgcgctggta
    15241 gcgaccattc gcacggaaga accgcatgat gcggtggcgc ggcaggtttt ccatggcgaa
    15301 ggcattctgg cctttttacc gcttagcgat ccgcatcttt gctcaattgt ctggtcactg
    15361 tcgccagagg aagcgcagcg gatgcagcag gcaagtgaag atgaatttaa tcgcgcgtta
    15421 aatatcgctt ttgataatcg cctgggctta tgcaaggttg agagcgcgcg tcaggtgttc
    15481 ccactgacgg ggcgttatgc gcgccagttt gccgcgcacc gtctggtgct agtgggcgac
    15541 gccgcgcata ccattcaccc gctggcgggg cagggagtaa atctcggctt tatggatgct
    15601 gcagagctga ttgctgaact gaaacggttg catcgtcagg ggaaagacat cgggcagtac
    15661 atttacctgc gccggtatga gcgtagccgc aagcacagtg cagcgctgat gctggctggt
    15721 atgcagggat tccgcgatct gttttccggt accaatccgg cgaaaaaact gctgcgtgat
    15781 attggtttga aactggccga cacgcttcct ggcgttaaac cacaacttat ccgccaggca
    15841 atgggattaa acgatttgcc tgaatggctg cgttaaaaat ttctcttctg ctgtttattt
    15901 gatacccatc acactttcat ctcccggttt tttcgccggg agattttcct catttgaaat
    15961 aaactaattt cacctccgtt ttcgcattat attttctaat gtcattattt tttgatttag
    16021 tgttttttga cattttttta gctcttaata ttgccttatt caaattagct ttctaatggc
    16081 atcatcttta tatagaaact ggtgtgtttt ttggtttttt attctgtcgc gattttggcg
    16141 ttttttaacc ataagctaat gtgatgatca attttacctt atggttaaca gtctgtttcg
    16201 gtggtaagtt caggcaaaag agaacgattg cgttggggac cgggagtggc tccgatgctg
    16261 ggtttcgtgg tgataatttc accatgaaaa gttgtcagcc ccgcttattc aatgaggaca
    16321 agatggcaca acagactcct ttgtacgaac aacacacgct ttgcggcgct cgcatggtgg
    16381 atttccacgg ctggatgatg ccgctgcatt acggttcgca aatcgacgaa catcatgcgg
    16441 tacgtaccga tgccggaatg tttgatgtgt cacatatgac catcgtcgat ctccgcggca
    16501 gccgcacccg ggagtttctg cgttatctgc tggcgaacga tgtggcgaag ctcaccaaaa
    16561 gcggcaaagc cctttactcg gggatgttga atgcctctgg cggtgtgata gatgacctca
    16621 tcgtctacta ctttactgaa gatttcttcc gcctcgttgt taactccgcc acccgcgaaa
    16681 aagacctctc ctggattacc caacacgctg aacctttcgg catcgaaatt accgttcgtg
    16741 atgacctttc catgattgcc gtgcaagggc cgaatgcgca ggcaaaagct gccacactgt
    16801 ttaatgacgc ccagcgtcag gcggtggaag ggatgaaacc gttctttggc gtgcaggcgg
    16861 gcgatctgtt tattgccacc actggttata ccggtgaagc gggctatgaa attgcgctgc
    16921 ccaatgaaaa agcggccgat ttctggcgtg cgctggtgga agcgggtgtt aagccatgtg
    16981 gcttgggcgc gcgtgacacg ctgcgtctgg aagcgggcat gaatctttat ggtcaggaga
    17041 tggacgaaac tatttctcct ttagccgcca acatgggctg gactatcgcc tgggaaccgg
    17101 cagatcgtga ctttatcggt cgtgaagccc tggaagtgca gcgtgagcat ggtacagaaa
    17161 aactggttgg tctggtgatg accgaaaaag gcgtgctgcg taatgaactg ccggtacgct
    17221 ttaccgatgc gcagggcaac cagcatgaag gcattatcac cagcggtact ttctccccga
    17281 cgctgggtta cagcattgcg ctggcgcgcg tgccggaagg tattggcgaa acggcgattg
    17341 tgcaaattcg caaccgtgaa atgccggtta aagtgacaaa acctgttttt gtgcgtaacg
    17401 gcaaagccgt cgcgtgattt acttttttgg agattgattg atgagcaacg taccagcaga
    17461 actgaaatac agcaaagaac acgaatggct gcgtaaagaa gccgacggca cttacaccgt
    17521 tggtattacc gaacatgctc aggagctgtt aggcgatatg gtgtttgttg acctgccgga
    17581 agtgggcgca acggttagcg cgggcgatga ctgcgcggtt gccgaatcag taaaagcggc
    17641 gtcagacatt tatgcgccag taagcggtga aatcgtggcg gtaaacgacg cactgagcga
    17701 ttccccggaa ctggtgaaca gcgaaccgta tgcaggtggc tggatcttta aaatcaaagc
    17761 cagcgatgaa agcgaactgg aatcactgct ggatgcgacc gcatacgaag cattgttaga
    17821 agacgagtaa cggctttatt cctcttctgc gggagaggat cagggtgagg aaaatttatg
    17881 cctcaccctc actctcttcg taaggagaga ggttcacaat tcactgcacg tttcaggaac
    17941 catcgctcat gacacagacg ttaagccagc ttgaaaacag cggcgctttt attgaacgcc
    18001 atatcggacc ggacgccgcg caacagcaag aaatgctgaa tgccgttggc gcacaatcgt
    18061 taaacgcgct gaccggccag attgtaccga aagatattca gcttgcgacc ccgccgcagg
    18121 ttggcgcacc ggcgaccgaa tacgccgcac tggcagaact caaggctatt gccagtcgca
    18181 ataaacgctt cacgtcttac atcggcatgg gttacaccgc cgtgcagcta ccgccggtta
    18241 tcctgcgtaa catgctggaa aatccgggct ggtataccgc gtacactccg tatcaacctg
    18301 aagtctccca gggccgcctt gaagcactgc tcaacttcca gcaggtaacg ctggatttga
    18361 ctggactgga tatggcctct gcttctcttc tggacgaggc caccgctgcc gccgaagcaa
    18421 tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa ccgcttcttc gtggcttccg
    18481 atgtgcatcc gcaaacgctg gatgtggtcc gtactcgtgc cgaaaccttt ggttttgaag
    18541 tgattgtcga tgacgcgcaa aaagtgctcg accatcagga cgtcttcggc gtgctgttac
    18601 agcaggtagg cactaccggt gaaattcacg actacactgc gcttattagc gaactgaaat
    18661 cacgcaaaat tgtggtcagc gttgccgccg atattatggc gctggtgctg ttaactgcgc
    18721 cgggtaaaca gggcgcggat attgtttttg gttcggcgca acgcttcggc gtgccgatgg
    18781 gctacggtgg cccacacgcg gcattctttg cggcgaaaga tgaatacaaa cgctcaatgc
    18841 cgggccgtat tatcggtgta tcgaaagatg cagctggcaa taccgcgctg cgcatggcga
    18901 tgcagactcg cgagcaacat atccgccgtg agaaagcgaa ctccaacatt tgtacttccc
    18961 aggtactgct ggcaaacatc gccagcctgt atgccgttta tcacggcccg gttggcctga
    19021 aacgtatcgc taaccgcatt caccgtttga ccgatatcct ggcggcaggc ctgcaacaaa
    19081 aaggtctgaa gctgcgccat gcgcactatt tcgacactct gtgtgtggaa gtggccgaca
    19141 aggcgggcgt actggcgcgt gccgaagcgg ctgaaatcaa cctgcgtagc gatatcctga
    19201 acgcggttgg gatcaccctt gatgaaacca ccacgcgtga aaacgtaatg cagcttttca
    19261 gcgtgctgct gggcgataac cacgggctgg agatcgacac gctggacaaa gacgtggctc
    19321 acgacagccg ctctattcag cctgcgatgc tgcgcgacga cgaaatcctc actcatccgg
    19381 tgtttaatcg ctaccacagc gaaaccgaaa tgatgcgcta tatgcactcg ctggagcgta
    19441 aagatctggc gctgaatcag gcgatgatcc cgctgggttc ctgcaccatg aaactgaacg
    19501 ccgccgccga gatgatccca atcacctggc cggaatttgc cgaactgcac ccgttttgcc
    19561 cgccggaaca ggctgaaggt tatcagcaga tgattgcgca gctggctgac tggctggtga
    19621 aactgaccgg ttacgacgcc gtttgtatgc agccgaactc cggcgcacag ggcgaatacg
    19681 cgggcctgct ggcgattcgt cattatcatg aaagccgcaa cgaagggcat cgcgatatct
    19741 gcctgatccc ggcttctgcg cacggaacta accccgcttc tgcacatatg gcaggaatgc
    19801 aggtggtggt tgtggcgtgt gataaaaacg gcaacatcga tctgactgat ctgcgcgcga
    19861 aagcggaaca ggcgggcgat aacctctcct gtatcatggt gacttatccc tctacccacg
    19921 gcgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt gcatcagttc ggcggtcagg
    19981 tttaccttga tggcgcgaac atgaacgccc aggttggcat cacctcgccg ggctttattg
    20041 gcgcggacgt ttcgcacctc aacctgcata aaactttctg cattccgcac ggcggtggtg
    20101 gtccgggtat gggaccgatc ggtgtgaaag cgcatctggc accgtttgta ccgggtcata
    20161 gcgtggtgca aatcgaaggc atgttaaccc gtcagggcgc agtttctgcg gcaccgttcg
    20221 gtagcgcctc tatcctgcca atcagctgga tgtacatccg catgatgggc gcagaagggc
    20281 tgaaaaaagc aagccaggtg gcaatcctca acgccaacta tattgccagc cgcctgcagg
    20341 atgccttccc ggtgctgtat accggtcgcg acggtcgcgt ggcgcacgaa tgtattctcg
    20401 atattcgccc gctgaaagaa gaaaccagca tcagcgagct ggatattgcc aagcgcctga
    20461 tcgactacgg tttccacgcg ccgacgatgt cgttcccggt ggcgggtacg ctgatggttg
    20521 aaccgactga atctgaaagc aaagtggaac tggatcgctt tatcgacgcg atgctggcta
    20581 tccgcgcaga aattgaccag gtgaaagccg gtgtctggcc gctggaagat aacccgctgg
    20641 tgaacgcgcc gcacattcag agcgaactgg tcgccgagtg ggcgcatccg tacagccgtg
    20701 aagttgcggt attcccggca ggcgtggcag acaaatactg gccgacggtg aaacgtctgg
    20761 atgatgttta cggcgaccgt aacctgttct gctcctgcgt accgattagc gaataccagt
    20821 aattcactga ttcgactatt ttctaaaggc gcttcggcgc ctttttagtc agatgacaaa
    20881 gtacaaaagt gctcagacag tcccctcgcc cctttgggga gagggttagg gtgaggggaa
    20941 caggccggca ctggcgcgaa tatttaccct caccccggcc atctccctga aagggcgagg
    21001 ggaaaagcgt gccaacattg aagattgagc cagtttgtta gcaatctcaa agatacgtca
    21061 acgaattaat ttttctcgga aaaacaaatg gctatagcac ttgtgaccgg tggcagtcgc
    21121 ggcatcgggc gggcaactgc attactgttg gcgaaagaag agtatacggt ggcggttaat
    21181 tatcaacaaa acctccatgc ggcgcaggaa gtggtgaact taatcacgca agccggtggc
    21241 aaggcattcg tgctccaggc ggatatcagc gacgaaaatc aggtcatcgc aatgtttaca
    21301 gcaatcgatc agcacgatga accgctagca gcgctggtca ataacgccgg gatcttgttt
    21361 acccagtgca ccgttgaaaa ccttaccgca gagcgaatca accgagtact ttccaccaac
    21421 gtgacgggat attttctctg ctgccgcgag gcggtaaaac gcatggcgct taaaaatggt
    21481 ggcagtggcg gcgctatcgt caatgtctct tcggtggcct cacggttggg ttcgccaggg
    21541 gaatatgttg attacgcggc atcgaaaggg gcgattgata cgttaaccac cggactatcg
    21601 ctggaagtcg ccgcgcaggg gatccgcgtt aactgcgtgc ggccagggtt tatttatacc
    21661 gaaatgcacg ccagcggcgg cgagcttgga cgcgtcgatc gcgttaagtc gaacatcccc
    21721 atgcagcgtg gtggacaggc agaagaggtc gcgcaggcca ttgtctggct actaagtgat
    21781 aaagcctctt acgtcacggg aagttttatc gatttggcgg gcgggaaata aaacagggaa
    21841 gttgtctgac cggatgcaac aagtattgca tccggtactt catcgactta aagcttctcg
    21901 ccgttgctgg caatcacctc tttgtaccag ttaaagctct tcttacgtga acgcgacata
    21961 tcgccagtac cgtcgtcatg tttattcaca tagataaagc cgtagcgttt gctgtactgc
    22021 ccggtagtga atgacacgca gtcgatgcag ccccacggtg tgtagcccat cagatccaca
    22081 ccatcgtaag tcactgcttt tttcatctct tcaatatggg cgcgcaggta gtcaatgcgg
    22141 tagtcgtcgt tgatgctgcc atcttcttcc actttgtcgt aagcgccaaa accgttttcg
    22201 acaataaaca gcggcctctg ataacgctca tacagttcgc aaagggcata gcgcagacct
    22261 actggatcaa tctgccagcc ccagtcagac gctttaacat acgggttcgg tacgctgcct
    22321 tcaaaaccag agatcgcatc gccggtgccg ccttcggcct tcactgcgtt gatcatgtaa
    22381 tagctgaaac caagataatc gcaggtgcct tcacgcagta catccagatc gccatcttcc
    22441 attttgatgt taaatccgcg acgctcccac tcgttcaaca cataggacgg gtaatagccg
    22501 cgtagctgca catcggtaaa gacgtagcgt tcgcgcatcg actcctgagc gaacatcaca
    22561 tcgtccgggt tacaggagta aggatagagc ggcaccatcg ccagcataca gccgactttc
    22621 atctccgggt taatgcgacg cgcggctttc actgccaggg cgctggcgac aaactggtga
    22681 tgcagcacct ggtacatcgt ctcttccggg ttgtcatgct cggtatacac cacgccggag
    22741 cagcagtaac caaacagcgg cgcacgccag ttacgctggt tgtttatttc gttgaaggtc
    22801 atccagtatt tgactttatg cttgtagcgt tcgaatacca cttccgcgaa acggacaaag
    22861 aaatcaacga ctttacggtt ggtccagcta ccgtattgct gcaccagatg cagcggtatt
    22921 tcaaagtggg agagggttat caccggttcg atgttgtatt tcagcagttc atcgaacaga
    22981 gagtcgtaaa acttcagccc ttcttcgttt ggctgagctt catcaccttt tggaaaaata
    23041 cgcgtccagg cgatggaagt ccgaaaacat ttgaagccca tttcggcaaa tagcttgatg
    23101 tcttccttgt agtgaccata aaaatcaacg gcttcatggt ttggatagta ttttcctggc
    23161 aagacttctt tggtgatttc gcgcggcacg ccgtgtgcgc caccggtcag aacgtcacaa
    23221 atgctcggtc ctttgccgcc tttgttccag ccgccttcga cctgatgagc ggcaacggcg
    23281 ccaccccata agaaatcttt cggtaaggtg agttttttca ctatcatagg ctccagatga
    23341 gtcaattctt acaggaaata gtaccatgca gccaaacgac atcacttttt ttcaacgttt
    23401 ccaggatgac attctggctg ggcgtaaaac catcaccatc cgcgacgagt ctgaatcgca
    23461 cttcaaaacg ggtgatgtgc ttcgtgtcgg acgttttgaa gatgacggtt atttttgcac
    23521 gattgaagtc accgcaacct caaccgtaac gctggatacg ctgacggaaa aacatgcaga
    23581 gcaggaaaat atgaccctga ctgaactgat aaaggtcatt gccgacatct atcccggtca
    23641 gacacagttt tatgtgattg aatttaaatg tctttaaatt gaactcacgc ctgttagttg
    23701 aaatttctca tttaatttca tgatttttag gagattagtc tcaaatcaga atttatttta
    23761 gctaacaggt gttcactgga actattctca gttacgctgg agaggtatac atgtgcgtgt
    23821 acgtttctcc ggagtaagtt atggttcaga agcccctcat taagcaggga tattcgctgg
    23881 cagaggaaat tgccaacagc gtcagtcacg gcattgggtt ggtgtttggt atcgttgggc
    23941 tggtgttgct actggttcag gcggtggatc tcaatgccag cgccacagcg ataaccagct
    24001 acagcctcta tggcggcagt atgatcctgc tgttcctcgc ttcgacgctc tatcacgcca
    24061 ttcctcatca gcgggcaaaa atgtggctga agaaatttga ccattgcgct atttacctgt
    24121 tgattgccgg aacctacacg ccgtttttgc tggtggggct ggattctccg ttagcgcgcg
    24181 ggttgatgat tgttatctgg agcctggcat tgctggggat tctgtttaaa ctgaccatcg
    24241 cgcaccgatt caaaatttta tctctggtga cctatctggc gatgggctgg ctgtcgctgg
    24301 tggtaattta tgaaatggca gttaagctcg cggcgggcag cgttacctta ctggcggtag
    24361 gcggcgtggt ttattcgctc ggagtgattt tctatgtctg caaacgcatt ccctacaatc
    24421 atgccatctg gcacggcttc gtgctcggcg gcagtgtgtg ccactttctg gcgatctatt
    24481 tgtatattgg gcaggcgtaa tgggtatgcc tttgtaggcc tgataagacg cgtcagcgtc
    24541 gcatcaggcg ataaatgcct gatgcgatac agagatttac tcttcgagtg aatacggcag
    24601 cggctcgata tgcagtgtat tcgcatcgtc gcgtacgcgg aagatgctgt ccggttccat
    24661 atcgttattc atgacgacct gtaccacgac ctgaccatct tccagtttta ccgcagccag
    24721 caccgtaccg gtacgacgcc agttctcgcc cattttcagc tctaagtctt caccagcttc
    24781 cggcagtcgg ctggcgctac ctgccagcaa ccagagcgca cgtttattgg caccacggaa
    24841 ttttgctcgc gccaccatct cttgtccggt ataacagcct ttcttaaaac tgataccgcc
    24901 cagcgcctgg agattggtcg cctgtgggat aaactgcccg ctgttcgcgg catcaatcac
    24961 cgggaaaccc gcttcaatgt ttaatgccag ccactgttgg ctattgttca gttccgcttc
    25021 accgcgcagt ttatcggtca gcatattggc agtagcttca tcggttacga tcaggaaacg
    25081 ttctgccggg tgttcaaacc atagcaaagt ggtcgcgcct tctttaacta cctgtttttc
    25141 tctcgaaggt agttcactaa agagattggc gagcgcggcg cgcgcctgaa aaccggcaac
    25201 accaagcagc acacgctcgt cgtctggcgc gatggtcact ttagagaaca ccgcatattt
    25261 tttcagttca gtcagctgcg gttcacgcac gctgcgccgt tcaatccacg caaagccatc
    25321 gccgtcgcgg aacagacgta aattgctcca cattttacct ttggcgtcgc aatgggcggc
    25381 gagcaggtgc tgattttctg tcatctggct gacatctgct gtcacctgac cctgcatata
    25441 tttttcgctg tccgcgccag taatggtggc aagcgcccag tcatcaagcg tcatcagcgt
    25501 cagcggtaaa cgggcagaag ccgtaggctg acggggagga aaaggtgtaa aagccataat
    25561 agagtcctga gttgcttaac ggcgtattaa taactaatgg taaaagagct atgaggcaat
    25621 gcaagtgctt tcactacgcg atctgtgcct tctgcgaggc gacttccaaa aaaatgaagc
    25681 tcttgaattg ttgtgaatta atttcgcgat cgcgatggtg tttactgata ttcccgtaaa
    25741 agaagtcgaa aaacacgtta caatagccgg gtactggatt ttcgtgagac aggaagaaga
    25801 tggacattaa caacaaagcc cgcattcatt gggcatgccg ccgtggtatg cgcgaactcg
    25861 atatttcaat catgccgttt ttcgaacatg agtacgacag cttaagcgat gacgaaaaac
    25921 gcatctttat tcgtctgctg gaatgtgacg atccggacct gtttaactgg ctgatgaatc
    25981 acggtaaacc ggccgatgca gaactggaaa tgatggtccg actcatccag acacggaacc
    26041 gggaacgtgg tcctgtggca atctgatttg cgcgtctcct ggcgcgcaca gtggctttcc
    26101 ttgctgattc atgggctggt tgccgctgtt attttactca tgccctggcc gctcagttac
    26161 accccgttat ggatggtgtt actttcgctg gtggtatttg attgcgttcg cagccagcgg
    26221 cgtattaatg ctcgccaggg ggaaattcga ttgttgatgg acgggcgttt gcgttggcaa
    26281 gggcaggagt ggagcatcgt caaagcaccg tggatgatta agagcggcat gatgctgcgt
    26341 ttacgttctg atggcggtaa acggcaacat ttatggctgg cagccgacag catggacgaa
    26401 gctgaatggc gggatttacg gcggattttg ttgcaacaag agacgcaaag ataaatatga
    26461 cggcaaagca tatgcgctaa gccgtcagtg agatcaggcg taatgctctg ccatttcgtt
    26521 gaggatttgc tcgcaccagc tctgaatacg ctcgtcgcta aggtcatact ggttagtttc
    26581 atccagcgcc agacccacga acagttgccc gtcagcaatc accggtttcg ggctggtaaa
    26641 ttcatatcct tccgttggcc agtagccgac gaacttcacg cctttggtcg agagtttgtc
    26701 atgcagcata ccgagcgcat cgaggaacca ctcgccgtat cccagttgat cgccaagccc
    26761 atacagcgca acaattttac cttcaaggtt caggtcgtcg agctgatccc agacggcttc
    26821 ccagtcttcc tggatttcgc caaaatccca ggtcgggata cccagaatga gcacatcgta
    26881 ctgctccatt aatttcgggg agtcgtcctt gaggttatgt aaggtcacca gttctgggcc
    26941 gataatatcg cggatttttt ctgccgccat ttcggtgtaa caggtgctgg aaccgtaaaa
    27001 aagacccata ttcataacgt aagcttctcg attctgctgt ggcttttgcg gctagtgtat
    27061 cagaatcgct ttagtcacgg cacaataagg cataatggat caaatgagtg accataaggg
    27121 gcgcaagtga aacaggatct ggcacgcatc gagcagtttc ttgatgctct gtggctggaa
    27181 aaaaatctgg ctgaaaatac gttgaacgct taccgtcgcg atctgtcaat gatggtggag
    27241 tggttgcatc accgcgggtt gacgctggcg acggcgcaaa gtgacgattt gcaggcatta
    27301 ctggcagaac ggctagaggg cgggtataaa gccaccagct cagcgcgttt gctgagtgca
    27361 gtgcgccgat tgttccagta tctttatcgc gaaaagtttc gtgaagacga tcccagtgcg
    27421 catctcgctt caccgaaatt gccacagcgt ttgccaaaag atttaagcga agcgcaggtc
    27481 gaacgtttat tacaggcacc attaattgat cagccactgg agctacgcga taaagccatg
    27541 cttgaagtgt tgtatgctac cgggctgcgt gtctctgaac tggtcgggct gacaatgagt
    27601 gatatcagcc tacgtcaggg cgtagtgcgg gtcattggta aaggcaacaa agagcgcctg
    27661 gtgccgttag gtgaagaggc cgtttactgg ctggaaacct atctggaaca tgggcgtccg
    27721 tggctgttga atggtgtgtc aattgacgtg ttgtttccca gccagcgtgc gcagcagatg
    27781 acgcgacaga ccttctggca ccgcattaaa cattatgccg tgctggcggg tatcgacagc
    27841 gaaaagctgt caccgcatgt gttgcgtcac gcttttgcca ctcatttatt aaatcatggt
    27901 gcggatttac gcgtggtgca gatgctactg ggccacagcg atctctccac cacgcaaatt
    27961 tatacgcatg tcgctaccga gcgtctgcgg caacttcatc aacagcatca cccgcgggcg
    28021 tgatgtctga aaagaacggg aagatttatg aagaaaggtt ttatgttgtt tactttgtta
    28081 gcggcgtttt caggctttgc tcaggctgat gacgcggcaa ttcaacaaac gttagccaaa
    28141 atgggcatta aaagcagcga tattcagcca gcacccgtgg ctggcatgaa gactgtgctg
    28201 actaacagcg gtgtgttgta catcaccgat gacggtaaac atatcattca ggggccaatg
    28261 tatgacgtta gtggcgcggc tccggtcaat gtcaccaata aaatgctgct aaagcagttg
    28321 aatgcgcttg aaaaagagat gatcgtttat aaagcgccgc aggaaaaaca cgtcatcacc
    28381 gtgtttactg atattacctg tgggtactgc cacaaactgc atgagcaaat ggcagactac
    28441 aacgcgctgg ggatcaccgt gcgttatctt gctttcccgc gccaggggct ggagagccag
    28501 gctgagcaac aaatgaaagc catctggtgt gcgaaagata aaaacaaagc gtttgatgat
    28561 gtgatggctg gtaaagctgc cactccggca agttgcgata tcgatattgc cgatcattac
    28621 gcacttggcg tccagcttgg cgttagcggc actccggcaa ttgtgctgag caatggcaca
    28681 cttgttccgg gttaccagcc gccgaaagag atgaaagaat tcctcgacga acaccaaaaa
    28741 atgaccagcg gtaattaatt cgcgtgaaac aacagataca acttcgtcgc cgtgaagtcg
    28801 atgaaacggc agacttgccc gctgaattgc ctcccttgct gcgccgttta tacgccagcc
    28861 ggggagtacg cagtgcgcaa gaactggaac gcagtgttaa aggtatgttg ccctggcagc
    28921 aactaagcgg tgtcgaaaag gccgttgaga tcctttacaa cgcttttcgc gaaggaacgc
    28981 ggattattgt ggtcggtgat ttcgacgccg acggcgcgac cagcacggct ctaagcgtgc
    29041 tggcgatgcg ctcgcttggt tgcagcaata tcgactacct ggtaccaaac cgtttcgaag
    29101 acggttacgg cttaagcccg gaagtggtcg atcaggccca tgcccgtggc gcgcagttaa
    29161 ttgtcacggt tgataacggt atttcctccc atgcgggggt tgaacacgct cgctcgttgg
    29221 gcattccggt tattgttacc gatcaccatt tgccaggcga aacattaccc gcggcggaag
    29281 cgatcattaa ccctaacttg cgcgactgta atttcccgtc gaaatcactg gcaggcgtgg
    29341 gtgtggcgtt ttatctgatg ctggcgctgc gcaccttttt gcgcgatcag ggctggtttg
    29401 atgagcgcgg catcgcaatt cctaacctgg cagaactgct ggatctggtc gcgctgggga
    29461 cagtggcgga cgtcgtgccg ctggacgcta ataatcgcat tctgacctgg caggggatga
    29521 gtcgcatccg tgccggaaag tgccgtccag ggattaaagc gctgctggaa gtggcaaacc
    29581 gtgatgcaca aaaactcgcc gccagcgatt taggttttgc gctggggcca cgtctcaatg
    29641 ctgccgggcg actggacgat atgtccgtcg gtgtggcgct gttgctgtgc gacaacatcg
    29701 gcgaagcgcg cgtgctggca aatgaactcg atgcgctaaa tcagacgcga aaagagatcg
    29761 aacaaggaat gcagattgaa gccttgaccc tgtgcgagaa actggagcga agtcgcgaca
    29821 cgctacccgg cgggctggca atgtatcacc ccgaatggca tcagggcgtt gtcggtattc
    29881 tggcttcgcg catcaaagag cgttttcacc gtccggttat cgcctttgcg ccagcaggtg
    29941 atggtacgct gaaaggttcc ggtcgctcca ttcaggggct gcatatgcgt gatgcgctgg
    30001 agcgattaga cacactctac cccggtatga tgctgaagtt tggcggtcat gcgatggcgg
    30061 cgggtttgtc gctggaagag gataaattcg aactctttca acaacggttt ggcgagctgg
    30121 ttaccgagtg gctggcccct tcgctattgc aaggcgaagt ggtgtcagac ggcccgttaa
    30181 gcccggccga aatgaccatg gaagtggcgc agctgctgcg cgatgctggc ccgtgggggc
    30241 agatgttccc ggagccgctg tttgatggtc atttccgtct gctgcaacag cggctggtgg
    30301 gcgaacgtca tttgaaagtc atggtcgaac cggtcggcgg cggtccgctg ctggatggta
    30361 ttgcttttaa tgtcgatacc gccctctggc cggataacgg cgtgcgcgaa gtgcaactgg
    30421 cttacaagct cgatatcaac gagtttcgcg gcaaccgcag cctgcaaatt atcatcgaca
    30481 atatctggcc aatttagcgt catcttctct ataaaaaaga gcgtggattg ggtacaatcc
    30541 cgctcttatc accgcatttt gactagctca ataaaagaaa tcagaccatg tttgaaatta
    30601 atccggtaaa taatcgcatt caggacctca cggaacgctc cgacgttctt agggggtatc
    30661 tttgactacg acgccaagaa agagcgtctg gaagaagtaa acgccgagct ggaacagccg
    30721 gatgtctgga acgaacccga acgcgcacag gcgctgggta aagagcgttc ctccctcgaa
    30781 gccgttgtcg acaccctcga ccaaatgaaa caggggctgg aagatgtttc tggtctgctg
    30841 gaactggctg tagaagctga cgacgaagaa acctttaacg aagccgttgc tgaactcgac
    30901 gccctggaag aaaaactggc gcagcttgag ttccgccgta tgttctctgg cgaatatgac
    30961 agcgccgact gctacctcga tattcaggcg gggtctggcg gtacggaagc acaggactgg
    31021 gcgagcatgc ttgagcgtat gtatttgcgc tgggcagaat cgcgtggttt caaaactgaa
    31081 atcatcgaag agtcggaagg tgaagtggcg ggtattaaat ccgtgacgat caaaatctcc
    31141 ggcgattacg cttacggctg gctgcgtaca gaaactggcg ttcaccgcct ggtgcgtaag
    31201 agcccgtttg actccggcgg tcgtcgccac acgtcgttca gctccgcgtt tgtttatccg
    31261 gaagttgatg atgatattga tatcgaaatc aacccggcgg atctgcgcat tgacgtttat
    31321 cgcgcgtccg gcgcgggcgg tcagcacgtt aaccgtaccg aatctgcggt gcgtattacc
    31381 cacatcccga ccgggatcgt gacccagtgc cagaacgacc gttcccagca caagaataaa
    31441 gaccaggcca tgaagcagat gaaagcgaag ctttatgaac tggagatgca gaagaaaaat
    31501 gctgagaaac aggcgatgga agataacaaa tccgacatcg gctggggcag ccagattcgt
    31561 tcttatgtcc ttgatgactc ccgcattaaa gatctgcgca ccggggttga aacccgcaat
    31621 acgcaggccg tgctggacgg cagcctggat caatttatcg aagcaagttt gaaagcaggg
    31681 ttatgaggaa ccaacatgtc tgaacaacac gcacagggcg ctgacgcggt agtcgatctt
    31741 aacaatgaac tgaaaacgcg tcgtgagaag ctggcgaacc tgcgtgagca gggaattgcc
    31801 ttcccgaacg atttccgtcg cgatcatacc tctgaccaat tgcacgcaga attcgacggt
    31861 aaagagaacg aagaactgga agcgctgaac atcgaagtcg ccgttgctgg ccgcatgatg
    31921 acccgccgta ttatgggtaa agcctctttt gttaccctgc aggacgttgg cggtcgtatt
    31981 cagctgtacg ttgcccgtga cgatcttccg gaaggcgttt acaacgagca gttcaaaaaa
    32041 tgggatctcg gcgacatcct cggcgcgaaa ggtaaactgt tcaaaaccaa aaccggcgaa
    32101 ctgtctatcc actgcaccga gctgcgtctg ctgaccaaag cactgcgtcc gctgccggac
    32161 aaattccacg gcttgcagga tcaggaagcg cgctatcgtc agcgttatct ggatctcatc
    32221 tctaacgatg aatcccgcaa cacctttaaa gtgcgttcgc agatcctctc tggcattcgc
    32281 cagttcatgg taaatcgcgg ctttatggaa gttgaaacgc cgatgatgca ggtgatccca
    32341 ggcggtgccg ctgcgcgtcc gtttatcacc catcataacg cgctggatct tgacatgtac
    32401 ctgcgtatcg cgccggaact gtacctcaag cgtctggtgg tcggtggctt cgagcgtgta
    32461 ttcgaaatca accgtaactt ccgtaacgaa ggtatttccg tacgtcataa cccagagttc
    32521 accatgatgg aactctatat ggcttacgca gattacaaag atctgatcga gctgaccgaa
    32581 tcgctgttcc gtactctggc acaggatatt ctcggtaaga cggaagtcac ctacggcgac
    32641 gtgacgctgg acttcggtaa accgttcgaa aaactgacca tgcgtgaagc gatcaagaaa
    32701 tatcgcccgg aaaccgacat ggcggatctg gacaacttcg actctgcaaa agcgattgct
    32761 gaatctatcg gcatccacgt tgagaagagc tggggtctgg gccgtatcgt taccgagatc
    32821 ttcgaagaag tggcagaagc acatctgatt cagccgacct tcattactga atatccggca
    32881 gaagtttctc cgctggcgcg tcgtaacgac attaacccgg aaatcacaga ccgctttgag
    32941 ttcttcatcg gtggccgtga aatcggtaac ggctttagcg agctgaatga cgcggaagat
    33001 caggcgcagc gcttcctgga tcaggttgcc gcgaaagacg caggtgacga cgaagcgatg
    33061 ttctacgacg aagattacgt caccgcactg gaacacggct taccgccgac agcaggtctg
    33121 ggaattggta tcgaccgtat ggtaatgctg ttcaccaaca gccataccat ccgcgacgtt
    33181 attctgttcc cggcgatgcg tccagtaaaa taagcattac gttatgctaa taaccccgac
    33241 aaatgtcggg gttttttaat tgtgctgcgc gaaagcagac aacaattttc ttgcttcact
    33301 attggctgcc tgcattacca tccacggact gaacgcccac ggcgtggcat caataccgtg
    33361 taatacatct gctaaatcac accattgata atccatcact tcatcatcgt tgatctgtaa
    33421 cgcactgttg gtgcgtgcgg caaataccgg acacacttca ttttccacaa tgccattcgg
    33481 atcggtggcg cgatagcgaa agtcaggata gatagattca ggaggcgtaa tttccacgcc
    33541 aagctcataa cggcaacggc ggatcactgc gtcttcgttg ctttctccca gttgtgggtg
    33601 cccacaaacc gagttagtcc acacgccagg ccatgctttt ttgctcagtg cgcggcgggt
    33661 aactaataat tgccccttgg cattaaacag ccaactggag aacgcgagat gtaagagggt
    33721 gtctgccgtg tgtgcggcat acttttccag cgtacccgtg ggaactccct gtgcattcaa
    33781 taaaatgacg tgttccgttt gcataatttc tcacatgtaa ttctgatcac tcatcattat
    33841 agataattga ag
//
LOCUS       DAJTNA010000022        33544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTNA010000022 DAJTNA010000000
VERSION     DAJTNA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33544
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.022"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            529..666
                     /locus_tag="OO161_000910"
     CDS             529..666
                     /locus_tag="OO161_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462071.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1266..2045
                     /gene="ipaJ"
                     /locus_tag="OO161_000911"
     CDS             1266..2045
                     /gene="ipaJ"
                     /locus_tag="OO161_000911"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5462072.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAF"
     gene            complement(2991..3920)
                     /gene="virB"
                     /locus_tag="OO161_000912"
     CDS             complement(2991..3920)
                     /gene="virB"
                     /locus_tag="OO161_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5462073.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4137..4373)
                     /locus_tag="OO161_000913"
     CDS             complement(4137..4373)
                     /locus_tag="OO161_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5462074.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4377..6278)
                     /gene="ipaA"
                     /locus_tag="OO161_000914"
     CDS             complement(4377..6278)
                     /gene="ipaA"
                     /locus_tag="OO161_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5462075.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6287..7285)
                     /gene="ipaD"
                     /locus_tag="OO161_000915"
     CDS             complement(6287..7285)
                     /gene="ipaD"
                     /locus_tag="OO161_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5462076.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSNK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKDKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSKKNGGYVVNINMTPIDNMLKSLDNLGGNDEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7336..8427)
                     /gene="ipaC"
                     /locus_tag="OO161_000916"
     CDS             complement(7336..8427)
                     /gene="ipaC"
                     /locus_tag="OO161_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5462077.1"
                     /translation="MEIQNTKPAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            complement(8447..10189)
                     /gene="ipaB"
                     /locus_tag="OO161_000917"
     CDS             complement(8447..10189)
                     /gene="ipaB"
                     /locus_tag="OO161_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5462078.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTKLSELDPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10195..10662)
                     /gene="ipgC"
                     /locus_tag="OO161_000918"
     CDS             complement(10195..10662)
                     /gene="ipgC"
                     /locus_tag="OO161_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5462079.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="OO161_000919"
     CDS             complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="OO161_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5462080.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="OO161_000920"
     CDS             complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="OO161_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5462081.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="OO161_000921"
     CDS             complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="OO161_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5462082.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSTESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHPPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEE
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            13562..15178
                     /locus_tag="OO161_000922"
     CDS             13562..15178
                     /locus_tag="OO161_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5462083.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNHLNNKSWEPINKNINY
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKGLNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKNLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15188..15550
                     /gene="ipgE"
                     /locus_tag="OO161_000923"
     CDS             15188..15550
                     /gene="ipgE"
                     /locus_tag="OO161_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5462084.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15550..16008
                     /gene="ipgF"
                     /locus_tag="OO161_000924"
     CDS             15550..16008
                     /gene="ipgF"
                     /locus_tag="OO161_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5462085.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LTYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16017..17132
                     /gene="mxiG"
                     /locus_tag="OO161_000925"
     CDS             16017..17132
                     /gene="mxiG"
                     /locus_tag="OO161_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5462086.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICRFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFFLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDYKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17140..17391
                     /locus_tag="OO161_000926"
     CDS             17140..17391
                     /locus_tag="OO161_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HCR5462087.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTAALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17404..17697
                     /gene="mxiI"
                     /locus_tag="OO161_000927"
     CDS             17404..17697
                     /gene="mxiI"
                     /locus_tag="OO161_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5462088.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17703..18428
                     /gene="mxiJ"
                     /locus_tag="OO161_000928"
     CDS             17703..18428
                     /gene="mxiJ"
                     /locus_tag="OO161_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5462089.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEYLTNEVIYVFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18425..18952
                     /locus_tag="OO161_000929"
     CDS             18425..18952
                     /locus_tag="OO161_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5462090.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18894..19589
                     /gene="mxiN"
                     /locus_tag="OO161_000930"
     CDS             18894..19589
                     /gene="mxiN"
                     /locus_tag="OO161_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5462091.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19582..19989
                     /gene="mxiM"
                     /locus_tag="OO161_000931"
     CDS             19582..19989
                     /gene="mxiM"
                     /locus_tag="OO161_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5462092.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDENQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19973..20401
                     /gene="mxiM"
                     /locus_tag="OO161_000932"
     CDS             19973..20401
                     /gene="mxiM"
                     /locus_tag="OO161_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5462093.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCVLKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20531..21163
                     /gene="mxiE"
                     /locus_tag="OO161_000933"
     CDS             20531..21163
                     /gene="mxiE"
                     /locus_tag="OO161_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5462094.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21150..22850
                     /gene="mxiD"
                     /locus_tag="OO161_000934"
     CDS             21150..22850
                     /gene="mxiD"
                     /locus_tag="OO161_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5462095.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNNNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22869..23936
                     /gene="mxiC"
                     /locus_tag="OO161_000935"
     CDS             22869..23936
                     /gene="mxiC"
                     /locus_tag="OO161_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5462096.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23949..26009
                     /gene="mxiA"
                     /locus_tag="OO161_000936"
                     /gene_synonym="virH"
     CDS             23949..26009
                     /gene="mxiA"
                     /locus_tag="OO161_000936"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5462097.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26022..26423
                     /gene="spa15"
                     /locus_tag="OO161_000937"
                     /gene_synonym="spaK"
     CDS             26022..26423
                     /gene="spa15"
                     /locus_tag="OO161_000937"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5462098.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26427..27719
                     /gene="spaL"
                     /locus_tag="OO161_000938"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26427..27719
                     /gene="spaL"
                     /locus_tag="OO161_000938"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5462099.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27812..28150
                     /gene="spa13"
                     /locus_tag="OO161_000939"
                     /gene_synonym="spaM"
     CDS             27812..28150
                     /gene="spa13"
                     /locus_tag="OO161_000939"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5462100.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28137..29015
                     /gene="spa32"
                     /locus_tag="OO161_000940"
     CDS             28137..29015
                     /gene="spa32"
                     /locus_tag="OO161_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5462101.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSNQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29009..29890
                     /gene="spa33"
                     /locus_tag="OO161_000941"
                     /gene_synonym="spaO"
     CDS             29009..29890
                     /gene="spa33"
                     /locus_tag="OO161_000941"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5462102.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29895..30545
                     /gene="spa24"
                     /locus_tag="OO161_000942"
                     /gene_synonym="spaP"
     CDS             29895..30545
                     /gene="spa24"
                     /locus_tag="OO161_000942"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5462103.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30560..30820
                     /gene="spaQ"
                     /locus_tag="OO161_000943"
     CDS             30560..30820
                     /gene="spaQ"
                     /locus_tag="OO161_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5462104.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30820..31590
                     /gene="spa29"
                     /locus_tag="OO161_000944"
                     /gene_synonym="spaR"
     CDS             30820..31590
                     /gene="spa29"
                     /locus_tag="OO161_000944"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5462105.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31597..32625
                     /gene="spa40"
                     /locus_tag="OO161_000945"
                     /gene_synonym="spaS"
     CDS             31597..32625
                     /gene="spa40"
                     /locus_tag="OO161_000945"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5462106.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32638..32880
                     /locus_tag="OO161_000946"
     CDS             32638..32880
                     /locus_tag="OO161_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462107.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10487 a         4859 c         6706 g        11492 t
ORIGIN      
        1 ttgttgactg cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
       61 tgcggaacgt tggaacctat atctccccga taccaaaggc gaaatgtaag tgacaaaatg
      121 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      181 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      241 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      301 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      361 tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt ctgcttgata tgggagctta
      421 tggaggctct ctgtacaagg ttagggagct aacaaagcag ggataaaaag tccgcctgat
      481 gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa atggtgcgat gaatgttcag
      541 gctatcttta tcttgatggt ggttcagctg tttggtaaag aaatcgctgt gacaatagaa
      601 gaactgcagg cagtactggt cccacatcgt ttgctcagtc aactggtgaa gttccgtagc
      661 gcgtaaatta atgctttgca tgaaacgtta ctggagtgga tgaaagaata cctgcatata
      721 tcattactgc gttggattac cagtacaaca gattgaatga actggacatg ttgatagacg
      781 gcattgaaaa gcagcttata agcatagtgc ggcagagtta attggagagc acttcgcctt
      841 ctgagtatta agtaagcatg gtggtacctg cggatactat ctattcactg tgctcggtcg
      901 gcagccttac tgaccaagga gttgggggag ttgataacaa agttgaaaaa acagctctca
      961 accaaggtgg ttattgtaac aatagcaaat aagctggtgt gacctgtatc acaataaccg
     1021 attatggacg taaatacgac aaaaatatgt cagtatcagg cggtattaat tgctgatgct
     1081 gatggacatt aactcttaat aaaagggtgg atgctgaaag gttgttatgg tgacggacgt
     1141 gatgacgacg gaaaagagta tgaggttttg gatttaaagt tcgttcgggg aataaggacc
     1201 cggtcagcta attctcatag tagttcgcat tgctggatgc aatactgtgc tgcaattact
     1261 cgtcgatgtc ggaacaacgg aagccttgca aacggggatg tattcataca ggtgttatgt
     1321 tatatggggt tttattgcag ggggctatac caagagaata tatgatatct catcagactg
     1381 atgtgagggt aaatgagaat agagtaaacg aacaaggttg ttttttggcg aggaagcaga
     1441 tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc tgcaaaggaa ttaggggttg
     1501 ataaaatacc acaatacaaa gggtctatgt ccgaaatgac aaggaagagt tctcttgatt
     1561 tggataatcg ttgtgagcgg gatttatact taataactag tggtaattat aatccgagaa
     1621 tacacaaaga taatatcgcg gatgccggtt attctatgcc tgataagata gtcatggcta
     1681 cgaggcttct tggattgaat gcttatgtcg ttgaagaaag taacattttt tcgcaagtaa
     1741 ttagttttat ttatcccgat gcaagggact tattaattgg catgggatgt aatatagtac
     1801 atcaacgaga tgtgttatct tctaaccaaa gagttttgga agctgttgct gtatccttta
     1861 ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc tgatggaagc tatatggatc
     1921 cagctgtagg agaaaattat tcttgttttt caactatgga attaggtgca agaagaagta
     1981 attctaactt tataggatat actaaaatag gtatttccat agttataact aatgaggctt
     2041 tttaaattgt cgttttttat ctgtgaagga gcttgaatat tttggggcgg ggggcataac
     2101 aaatgatatt actgagtaca aatttagcta tattgggcgg attagttgtg tgggaagacg
     2161 aaatgtttct cttgaaataa gtgaactccc tcaaggaatg attgtgtgct taaatattta
     2221 agtgctggta agtttgagaa ggctgacata ttttgcagag caaacagtga ttgacttgga
     2281 tgatattttt attttagtca ctcgttgtaa ttggagtatg ctttatttta tttgaaaata
     2341 gatgtatttt atacccgcaa tttaaactgt aagccctatg aggggtatgg atattataag
     2401 ttactctttg actaagcata acttttgggg ttagaaaaat aaatctcttg ttaataatta
     2461 atatgatttt aaaatcaaac ttgtttataa ttataaaata atgtaagtcg gtcattttat
     2521 gggaagatta ggagaataaa acagtaatta tatttgagaa agctgctaat atattcttaa
     2581 agcttttttg atactgtgaa cgaagtctgt taatggtgga ttttttgttt atcaccatgt
     2641 aaaaaaattg agtagtaaaa gcgttgactg ctaatcctcg ctgtttttat tgaaacgatt
     2701 aaaaatacgc tgaatatttt cggccagtca ctcatcttca cataaaattt tgaccatcat
     2761 gccgcatccc ttagttgcac aattcatttt caatgatatg aatattacta atttcaccag
     2821 ataatgttct cgtggtcagc agggtatgag ttgtcaccgt aattttaagg aaacacagtg
     2881 cgtggggatt gaataacaga tagccgacgg aattacccca ccggctgaaa gtcaagttca
     2941 acgaagattt ttattatctg aattgggcag tttacatcag tgttcgatgt ttatgaagac
     3001 gatagatggc gagaaattat atcccgaata gcttcatcta tttctctctg aactgttttg
     3061 tttattttag ataatgtaaa tattagagtt ctgtttgttt tattctcttt tctttttatg
     3121 taagtccgtt tgtctttaga aataaataac gagtcaactt ttagcgaagg gttttgcttt
     3181 ctgtttttgc ttttctttat gatgtttaaa atttcttttt tatatatgtc cgggggcaaa
     3241 tttgtatcaa ggtcttttat ttcttccttt aatattggta gtgtagaact aagagattca
     3301 ttagcctttt caagtccttt ataataattg aataatatct tgtagtcatt aaagttaagt
     3361 tctgacgcga ttggaaatag agatattatt tcctgtggaa cgcttgctgc ctgaaaggca
     3421 cgagtgactt tcgcgcgaga cagattctct tttttggcta tgtctttata ggacatccct
     3481 gatactttca gaaaattaag accaatacca agttctcgga tgctatgctc ttttgctgtt
     3541 tgtatatcgt ttgctagttt tctggcatca agagtagata tatattcttt tgaatataga
     3601 acttcaagat ctgctcctgc atatattgca gatgctcttc tacgagtgcc atccagaatt
     3661 tcaattctac catcaatctc ccttcctatt acagggaaga attgttgtag ctttatagtt
     3721 tttatgatat cggctaatga ttcttctgtt agggattctt gatcgcgtac attcgttttt
     3781 tggttgacga aggttaaatc ttggatcctt ttatgaggaa tcttggcttt gataaaggaa
     3841 actttattac cagaatggag tgtaaactct ttcttttggc cttcctttat acttaacaag
     3901 tcgttgcaca aatccaccat atcacaccct gtttattcat attgatttat attgacaaaa
     3961 gttaaatgca gtgtgagcaa gattgtatgt aactgttgat gatagtcaac ggtagtctta
     4021 aaaattacta ttttaatagc tggattgatg caatttatct ttattggata aaagaataat
     4081 attcttgtgg agctctgatg tgagagctcc atagatgaaa aaatgcttat caattactat
     4141 attgattttg tactattttt gactacagaa ataagatctc caatagtaat aatgtgttct
     4201 aatgcagatt catctatacg tagattgaac tcaaaactaa tcatatccag catttcaatc
     4261 agagaaagag aatcggcaga aagatcttca ataaatttag tttcttcaga tagtttgctg
     4321 tacgctatac catagcaaaa ggccactatt gataatattt tttcttttat catattttaa
     4381 tccttattga tattctttaa tacttttgat agggaagtgg ttacatcttt tgctgcttta
     4441 tatatattat tgttttcagc tgtaatatca ttgttatttt ttaaatcaga tatatcatct
     4501 gtaagtaatt ctgcagaggt atcgtcaatt tttgacaata ccttgctgag ggcgctagat
     4561 acttcttttg ccttttcata gatcgcatga tttttatcaa ttgtatctgc gttggtttct
     4621 gttacgtagt ttattgtatt tgcatctgta acttttaatt tgttgtttat tctgtgttct
     4681 gttgtggatt ttgtaatgtc ctttgatttt tcctgtaact ctcgttcttg tgttcctgat
     4741 ttagttccaa tgagagatat aagattactt agggagtttg ttactttttt tgaagcttca
     4801 aatatcgttt cccgtgttaa atatgtgtct ccatttcttg ataaaaaatc agtctgaagt
     4861 acttttgaat cagtgtttga gttgaattta ttgatttgcg aagctactct atgagaaaga
     4921 gaagcgctac ttttagttgt atctgatgag ttatcattac tgcttgtcac tccttccggg
     4981 tggagagggg gggtggttga agagttctgt atgttcagag gcctgtgtcc ccgagaaaga
     5041 gagttatttg tagatctttc ttgactactc gtagttaaca ggtctgaacc tattccatcc
     5101 gaattatctg gagtatggtt gaaaaccttt tctaaagctg aattatttgc aaatgtctgg
     5161 acagtatctg atatagagtg tggtagaacg gaagttactt tttgaactag tgctctttgt
     5221 ggatttagta atgagtttcc gttacgggat aatagatcgt ttgtctgaga agtaggagac
     5281 tgtgcatcat tttctgggtt ctcgtgatag ctattgtcat atactctgtt gtcaaaaact
     5341 ctattatcgt aagttctatt atcattattt atatgatagt gaattacagg ttgggatgtt
     5401 ttctcattgg ctccgggggt tggtccgcca tctggcatat cttcgggagg tgtagggagg
     5461 ccatcttgga taggatgcga atcttttata tcttctaaaa tatcattaag gacttttctc
     5521 aacagctcag gaaacacggc tactgtattt aagtctcttg acagtttacc tataccaaat
     5581 cctttactat caatagtatt tttacgttga ttgcgcaaac tatcaaaggc aagttttgcc
     5641 tgtgcgtcaa ctaaatcatc tagtttgaga gatgaaacag tttcattact taactttaat
     5701 tcagcaagaa tattattctt gataaatgat gatacttcag tattgaggag tttttgtccc
     5761 aatgataaag cttcaccaga tgtgtgttgt tgtgctaata gtgcaagtct gtgctcaatg
     5821 ttttgtcgta attgatggag tttcttattc tttgccgacc atggtccaaa ggcagaatct
     5881 tgggataatt tatcttttat aattagttcc gaatactgtg cgacaatttt ttgaagtccc
     5941 ttttcgtttt caaaattaag accattttga tgtactaagg agtacaaaac atttgaaaat
     6001 tctagaatct cgcttttggc atacgcattc attgagttgg aataaatgct tcttaacaca
     6061 tcttctttcc ctgaggaaga aaataaaaaa tcaagacatt tctgattaaa agacgttgca
     6121 aagttttctt tttcagacac tgatgctggt gtttttagag aagtttgcga actatggata
     6181 tcggatattt tgctttttaa ctcgctgtat tctgttgatg atggtgaagt tgccttatat
     6241 aagaatgttg gcgcttgagt attatttaca ttatgcatgg cgcacctcag aaatggagaa
     6301 aaagtttatc tgtatctgta catgagctta ttgtactact caaaaccttt actaaattat
     6361 caaaaatact attggcatta ctgtattttt gaactaaagt ttgaagatta tttttcattg
     6421 tttcatcttc ggcagagaat ccggcattcc atgcctgata ttttgcatta tctagcacaa
     6481 cctcgtcatt tccacctaga ttatctaagc tttttaacat attgtctatt ggggtcatgt
     6541 ttatattgac aacatatccc ccgttttttt tagatacctt gccgattgtt ccacctaatt
     6601 ctgtaagcca tttatttgct tgttccttac taacagtatt atttgctgga tatagcggtt
     6661 tatctttata tttatccttg agctttgtca attcgtcttt aagcgatttg acttgtaatt
     6721 tcacggagtt tccgtcgtta cctccgggag agatccagcc ggcaagactg gaaagaacag
     6781 cgctaaaatc ttgatacatt tgagtatatg aactaacggc atgttcatat actttcagat
     6841 actgttcatt aatattattg attgactttg caattttatc ccacagttct ctgtgagata
     6901 tcatttcata tccatcaagc tcggcttctt tcggggctga atgtaataat tctcttgcgt
     6961 ctttattaat tggatattct ttattggaaa gaatatccaa tagttgagcg gatttgttta
     7021 catccatgct aatctgagat gaatgtaatg ctatttcttc tagcgatgtt ttagtcaacg
     7081 ttttttgtga gagatctttc tttaatgcct gatttgttgt tctgatatta tgaagggtgt
     7141 cgttgagcat agtaagggaa cttacaggat gagaactggt cgttgttttt atatcagaat
     7201 taactgtttc ggttgatgaa ccgttggtat tgtttgggct gaatgatgag gtggaaatac
     7261 tattagtcag agttgttata ttcatggtta tttccttata ggaagagcca tatattatta
     7321 tggctcttgg atatattaag ctcgaatgtt accagcaatc tgactggctg tagaattcct
     7381 tgattggttg atgttgtcaa ttatattcaa taatttttgt attaattgat ttgtcgcttg
     7441 ggatgcttct ttagatactt gggatgcttc ctctgcttgt ttactgctgg cctgactgat
     7501 tagttgttct tcttcttcaa gagcagatgt ataacgccct cctgatgtgg atatattacc
     7561 agcaacggct gatgtctcca ttgttgcacg gccaatgttt tgtttttgct gagcagaaag
     7621 ggtattgagc tcgtaagctc ttctctgggt gtcaatttta tcctgcaaag aaatatcggg
     7681 agaactaaga gaagttttat gttcagttga cagggatata ttatctggtg ccagtttatt
     7741 tttacctaaa atttttgtgc tagagttagt ttgtggtgag gtgatatttg tatctatttg
     7801 tttatttaac cctaatttag aacctgcaag ctctttttca agagattgag cagtggcaag
     7861 gttctttctt aaggctcctt tttgttcgct aatccctgaa tgcgtttttt tggcaccgat
     7921 acccgttata cctacttgtg tgaccgctcc agtaatgctt gatgataggg ctgctaggcc
     7981 ttgccgaaca atgttctctg cagctgattt tgtagcatcg aacgcaatca atgacaattg
     8041 agagcccaat ctagtttctg cagtgcggag agcagaaagt agaacggcta cactaataat
     8101 taaagaaaca gcattagaag aaagactgga aatatccagg gtgttctctg gagtaattgt
     8161 gtgactcatt tctgaaagat ctttcatctt tttgtcagta aggcgagcaa tgatttctga
     8221 atcatgctga acctgctctg ataactttaa aagttgatca tcatttaatg tagttgttaa
     8281 tacgggattt ttaccgacat taagtggaat atgtgctgct aactgctgat aattttgtga
     8341 tttttgtgtt tcggaagaac tttgagtttg ttttgtggat atatctgtat ataaaatcgg
     8401 ggctggtttt gtgttttgaa tttccataac attctcctta tttgtatcaa gcagtagttt
     8461 gttgcaaaat tgcttttgca acatcagttc tattagcctg agagttggac attgaggcta
     8521 acagatctgc aattacttcc tgcaattggc cgaatttttc tattgcgtca ccgatgtatt
     8581 ttgacagttg ttcaacttga tatttcgata atgtcaggtc tgctagattt ttcgacgcgt
     8641 tgttttggaa aacagcagaa gcgacatttc ctcccgcttg tgtggcagag ttaacacttt
     8701 ctcctaataa aactgcttgg tttaaatggg tggaaataat ttgtttggat attacttcat
     8761 cacccgctgc accaagaaat ttatttaatc tggcaacagc attagtgatt aaatcgtcca
     8821 gttgagaaga aaaattcttg agaaactttg gtataaggtc tgtgagggtt ttacctatta
     8881 ttttgccaat attctctgca agttttgctg ctgcctgttt accaacagtg gctacgagaa
     8941 cgactgctgc aaccaagaca agagcgcctg cgattgcccc cagaatagag ccaatcattt
     9001 tggctttttt cgagtcgacg cccaagcctt cgagcatttt tgtaaatgca tctgaaagga
     9061 gtttgattaa gggttcaatg actgctttca tgatcggatt cagggcttgt tccatgaagg
     9121 aattgccggt cgctgcttgt actatagcat ccgtaaccat aagagctaaa ccaacagctg
     9181 ccagtgctag agatgctcct ccagaaaaag ctgctgcaac aacactaacg atagttaaaa
     9241 gtgccccaag tattttccca acacaaccca ttactctgtt gagttcttct gctttacgta
     9301 cttcagcagc atactcatca gattttctct ccatttcagt ttttcttgat tcttggagag
     9361 actggaatag agccagatca ttttttaaag attcttcatt attttttcca actagttgaa
     9421 taaaggttgc catcaattga gtaacactgg caagtccggt taatgatttc tgctgggttg
     9481 atagctgttc agcagatgct gtgtttgaaa atgcagaaaa agagtctatt tctttttcga
     9541 gttgcattga tttatctgta agtttgctat gaattgacag ggttttctgc tcaatcaatg
     9601 tcctgtcttt aactgctgcg tcttttttga tagtgagttg tatttcttcc cgtcttaatt
     9661 ttttattttc tggtgaatct gggtcgagtt cggataattt tgtttgaatt tggttaattt
     9721 tattttctag gtcttttatt ttagaatctg cgttttttag tttattaatt tgtttttcat
     9781 agtctctggt tagtccttca gtttcagata gaagagtatt aattttatca gagaattcta
     9841 ggtttttttg ctgtcttgcc tgttgctggg acttccaggc agtaatttta tttgttaatg
     9901 cagttaaaga tttttcaccg agtatttgaa taaggtttcc aattaaaagc gttagttggg
     9961 aacttgcatt taatgacttt ggtgctttaa gttcagggat taatatattc gaagttgagt
    10021 gtgcattagt tgtattaata tttttgttag cagtaagatc agcaattgta agagaaaata
    10081 atttgttagc tgcatcattt gcagcctgga tagtattgtc tccaagctca gtggaagcca
    10141 atattttggc aagaggcaaa ccagtggttg tggtgcttac attatgcata ataattactc
    10201 cttgatatcc tgaattgcgt ctaagtatga ttgtgctttt atttttaatt tttcatcatt
    10261 gctgtgttga attacgagtt cgaagcactc tttggctttc aagggggctt tcaaccgaag
    10321 ctggcattgt ccagtatgga ataccggtgt atagtcattt tttcctaatg caaaagcgac
    10381 agcataaagg tctgctgctt gttggaactg ttcttttatc tgataaatag ctgcgagtcc
    10441 cataatgtag tctacattgt aaaagtcgta tatacataaa aatctgaaga aaacttcagc
    10501 ttcctctatt cttcctttgt tgtaaaagtc ataagcatat gaataaatgt catccatcat
    10561 atcatcagga attgcattaa tatctttcag tgtagcgcca gagttaattg catcaattac
    10621 tgcagtagag atgctttcat tttcagtgat atttaaagac ataaggtctc ctttttgtct
    10681 gcattacttt aataagtata agatttaata taaacagatt taatttgtat tgctttgacg
    10741 gtatacagcc tttagaacct ctgcgcttat ttctatttgt aaaacttttg ttgattcatt
    10801 aaatatttta tcgtagacgg aactggatac tagatttgta agatccttca tttgtgtgtt
    10861 aaacaaatcc atgtcactgc tataacgttt taaaatcttt gctctgaggc cagatgaggc
    10921 aatctcgttt agtgctgtat gactgataga tgtttgtgct gatttttcat caagttttat
    10981 atccgggaat tttttttcaa tagctgcaaa tatgtttttt ttgttatctg aggtcaaaac
    11041 aactccattg tgtatggcca tttcatctat acttctgttt atcattgaag agacataaga
    11101 ggttcgctct tggctcatcc aacaaaatag ttgattcccc gaattactat cttttatttt
    11161 gtctatgacg ctgtccctga ttttatttat attcaccggg catccaaaat tcttctgagg
    11221 aaagcgttgt tttaagttaa aaacagataa tattttcttt acaagcttat tgtgacttaa
    11281 cgaatcaaat gatgggcgag gaatcgcgaa ttcaacctgt ggaagtattt tgtttagaat
    11341 ttgcatgata ccccctatat gttagttcac ttctgaagtg atgtttgcaa tggttagttt
    11401 aatacaaaat aaaaattcgt acaaagattc aatgagttca tcaggtgaaa gatatttttt
    11461 gtagtgtttc ttggtcaaaa cacattgatg agtatcaggg agaagacaga tagaagaacc
    11521 ataatctcta ctcaaagagt agttaaggat gagtatgtgt tctggattaa taatattttc
    11581 tgtaaaaagt tcatgttcat taagaagtcc ggtcaaaaag atgtattcat cattatctgt
    11641 tatacaaaca ggtatttgtt cttcgagcaa tataaaggct tgatttttat cgttaaaatc
    11701 aagctttgca cctgttattt cataaagttt atcatatagt ttgcgacaca tttctatggc
    11761 cttctatata ttagaatgag agttattcaa taaaagctta ttgacctgta ataagtcgtg
    11821 aatgtaaagt ttggttccaa gatctggcga tttaagagaa ttgtaataat caatagtttt
    11881 tattgacttt tcgataattt cttctttttt atctgttgct ccggttttta attcgtttaa
    11941 tacacgagac atattgctgg gtgctgattt tactaagtat gagtggcaag cgttgatgac
    12001 cgcatcaaag tcttctgaag atacattttc agtaggatgt ttgtaaagtg aaattgataa
    12061 aggcttgaat gtttttgggg ggtggataag gttaatttta ttgaaacttt taagatagct
    12121 acgtcgtaat tgagtcaatc tggaaagatc aaaatcagat tcaatatact tcattatctc
    12181 gttataagac tgattgagac tttcaatgcg ttgcctaact ttatttgcat acgcgtatac
    12241 attgttaggt gtagcaacca atttaacgtc agggagtgga aagtatattt cagaatttcc
    12301 tgctttaaga acattaagtg ccataccagc acagttttca gatttactga ttaatttata
    12361 atttgccaat tgtctgaaag catctgcttt cttttggcat ataaattttg acatgtttgt
    12421 ttcatcaagg ccaaaaaggt tatagctgat tttaccatcc tttgttttat tatcacctga
    12481 taatggaatg tatactttat cagcactgac cccccaataa ccataggggt cttttttttg
    12541 attagctctg ggaaaaaata tttttttgtt gttattttga tcttgttttg aatttaataa
    12601 gtttctaacc tcttggcctg catcaagtct ttcggctgtt tggtctgaaa tcatatttag
    12661 tttgtcttgt ttataggtat cgacactgta acctgagcgt ttttcaaaaa ggtagtttat
    12721 ttctgtatct tttgtaacat tcccgccggg ataccagctg agataggtat gagttggttc
    12781 ctgtagtaaa ttgtttttta tactgatact ggtatgccct aaatgggtta tgcctctatt
    12841 tccccagcaa cgaaacacta aatgtctttc aagatttttg gctgtaccga tgccatgaaa
    12901 actctctgcg gtattcaaca ttaatttgat gtctttacct gtaaaagaat gttgaattga
    12961 atttttgact gcttcatagg cggcaaacgc actttctgta gatttttcgc tagctaatgt
    13021 cttgaggaaa agcaataagg atttttgatt cgctagtcga taatttttta gttgttcatt
    13081 taatatctgt gaatttatct ttgcattaat agtgcttaat gttctgaaaa agagatcctt
    13141 taggttgaat ttctgtgcta ccaatatagg tccatgctct gtatcttcaa cgcgtatagg
    13201 cccttttgta ttgcttgcgt caatgaaatt gctaattttg agaatcatac tttattaact
    13261 ctccattacg tggtgattgt ggggatgtaa aatgtatttg atatataata tattcaatta
    13321 ataaatttag aaacttgagc ctgttaacat aatcaaattt tcttttgctg tacataatat
    13381 gtacctcgtg agcatatgta gtgctggttt catcatgaaa tcccacaagt taaagtgtct
    13441 gatatatcag gctcggagtg ttatagaaaa agagagaacc ctgttgaata agtatctttt
    13501 ttgttgtaaa acgaggtaca aaactgcaat caatgcccca tttatgactg aggataatta
    13561 aatgcacata actaatttgg gattgcatca ggtttcattt caaagcggag attcctataa
    13621 aggcgcagaa gaaaccggaa accaaaaagg tgtaagcgtg atttcatatc aaagagttaa
    13681 aaatggagag agaaataagg gggtcgaagc gcttaatcga ttatatttac agaaccaaac
    13741 aagcttaaca gggaaaggcc ttttgtttgc gcgtgacaga gctgcagttt tttacgaggc
    13801 aataaagttg gcaggtggtg atacgtcaaa aattaaagcc atgatggaac aattagatac
    13861 ctataaactt ggtgaagttg ataaaagaaa tattaatgag cttaataaag taataagtga
    13921 agagataaga gcacagctag gcattaaaaa taaaaaggaa ttgcaatcgg aaataaaaca
    13981 aatatttact aatcatttaa ataataagag ttgggaaccg atcaacaaaa atattaatta
    14041 tcatgggaaa aattatggtt ttcaattaac acccgcctct catatgaaga tagggaataa
    14101 aaatatattt gtcaaagagt ataatggaaa aggaatttgt tgtgcttcta cgagagagag
    14161 taagcatatt gcgaatatgt ggctttcaaa agtggtggat gacgagggga aggaaatttt
    14221 ttcgggtatc agacatggag ttatatcagc ctatggattg aaaaaaaact catctgaaag
    14281 agctgttgca gctcgtaata aagctgagga gttagcaagc gcagcattat atagcagaca
    14341 agaattatta tcacaggctt tgtctggtaa aacagtagat ttaaagattg tttcgacttc
    14401 tcttctgacg ccgaccagtt taactggggg agaggagagt atgttaaaag atcaggtaaa
    14461 ggcattaaaa gggttaaact ctaaaagggg ggaaccaacg aaattattaa ttcggaatag
    14521 tgatggtctt ctgcaagaag taaaggttaa tctaaaagtg gtgacattta actttggtgt
    14581 aaatgaatta gcgctaaaaa tgggcttagg ctggaggaat gttgacaagc ttaatggtga
    14641 atcaatatgt tctttgttgg gggataattt cctcaaaaat ggtgtgattg gaggttgggc
    14701 tgcagaagct atagaaaaaa atccaccatg taagaatgat gtgatatatc tggctaacca
    14761 gataaaagag attataaaca agaatttaca aaaaaatgat aatggagagc cgtataaatt
    14821 gtcacaaaga atggctctat tggcttatac tataggtgct gtaccttgct ggaattgtaa
    14881 gagtgggaag gacagaacag gtatgcagga tgctgaaatt aaaagagaaa taataagaaa
    14941 acatgaaaca gggcagtttt ctcaattaaa tagtaaatta tcctcagaag agaaaagatt
    15001 attttctact attctaatga atagtggtaa tatggaaatc caagagatga atactggtgt
    15061 gcctggaaat aaagttatga aaaaattacc gctatcttcg ttagagctgt cttatgctga
    15121 aagaataggg gaccccaaaa tatggaatat ggtgaaaggg tattcgtcat ttgtataaga
    15181 ggaatatatg gaagatttag cagatgttat ttgccgagcc ttgggtatcc cttcaattga
    15241 taccgatgat caagcaatca tgcttgatga tgatgtgtta atatatattg aaaaagaagg
    15301 agattcaata aatttattat gccctttttg tgcattgcct gaaaatataa atgatcttat
    15361 atatgcgttg agcctaaatt actcagaaaa gatatgttta gctactgatg atgaaggtgg
    15421 aagtctaatt gcgcgattag atcttaccgg gataaatgaa ttcgaggata tatatgttaa
    15481 tacagaatat tatatttcgc gtgtgcgttg gctgaaggat gaatttgcgc gaagaatgaa
    15541 ggggtattaa tgtcccgttt cgtatttatt tttctgtgtt ttatcccata tttagggaga
    15601 gctgattgtt gggataaggc tggtgaaagg tacaacattc catcgagttt gttaaaagcg
    15661 attgcggaaa aagaatccgg atttaataaa tttgcagtca atgttaataa caatggaagt
    15721 aaagattatg gtataatgca aataaatgac tttcattcta aaagacttag agaaatggga
    15781 tatactgagg aaatgctaat tagtcatcca tgtctttctg tacattatgc agcaaagtta
    15841 ttaaatgagt ttatgctgac gtatggaaga gggtgggagg ctgttggtgc ctataatgca
    15901 ggaacatctc cgaaaaagaa aaaagaaaga ttaaagtacg cagaggatat ctataaacgc
    15961 tatttgcgga tagcagcaga gagcaagcag aataatcgaa ggatataaga ggatttatgt
    16021 ctgaggcaaa gaactcaaat cttgcaccat tcaggttatt agtgaaactc acaaacggtg
    16081 tcggggatga gtttccgtta tattatggga ataacttaat tgttctcgga cggacgatag
    16141 aaacacttga gtttggaaac gataatttcc cagaaaatat tattccagta acagatagta
    16201 aatcggatgg tataatttat ttggccataa gtaaagataa tatctgtcga tttagtgatg
    16261 aaaaaggtga acaaattgat atcaattcac aatttaactc atttgaatat gacggagttt
    16321 cttttcaact taaaaatatg atggaggata aatcgagagt tcatatacta aacggaatgt
    16381 ataataaacc ctcggtgttt tttttctttg ctgttattgt tgtgttaatt ataatttttt
    16441 tcctctcttt aaagaaagat gaagtaaaag aaattgctga aattatagat gataagagat
    16501 atggaatagt caacacagga cagtgcaatt atattcttgc cgaaacacaa aatgatgcgg
    16561 tatgggcatc tgtagcctta aataaaaccg gatttaccaa atgcagatat attctggtgt
    16621 ccaacaaaga aataaataga attcaacaat atatcaatca acgattccca tatatcaatt
    16681 tgtatgtgtt gaatttagtg agtgataaag cggaattact ggtattttta agtaaagaaa
    16741 gaaatagttc aaaagatacc gaacttgaga aattgaaaaa tgctctgata gttgaatttc
    16801 cgtatatcaa aaatattaaa ttcaactatc ttagcgatca taatgcaagg gaggatgcta
    16861 aaggtatttt cacaaaggta aatgtccagt ataaagagat ttgtgaaact aataaagtta
    16921 catattctgt cagagaagaa ctgacagatg aaaagcttga gctcattaat agactgattt
    16981 ctgattataa aaatgtatat ggagatcagt atattgagtt ttctgtactt ttgatagatg
    17041 atgattttaa aggtaaatca tatcttaaca gcaaagacag ttatgtaatg ttgaatgata
    17101 aacactggtt ttttttagat aaaaataagt gaggataaaa tgagtgttac agtaccggat
    17161 aaagattgga ctctgagttc attatctgaa acttttgatg atggaactca aacattacaa
    17221 gggcaactaa cagcggcact aaatgcatta gctgaaaatc cttcgaatcc acagttgctg
    17281 gctgaatacc aaagtaaatt atctgaatat acattatata ggaacgcgca atctaatact
    17341 gtgaaagtga ttaaggatgt tgatgctgca attattcaaa acttcagata atagggagca
    17401 ttcatgaatt acatttatcc agtcaatcag gttgatatta tcaaagccag tgattttcaa
    17461 tctcaagaga tatcaagcct ggaagacgtc gtgtcggcta aatatagtga tattaagatg
    17521 gatacagata ttcaagtatc acaaataatg gagatggtaa gcaatccaga atcattaaac
    17581 ccagaatctt tggccaagtt acagacgacg ctctcaaatt attcaatagg agtatcatta
    17641 gctggcacgt tagcaagaaa aacagtttcg gctgttgaaa ctttattaaa gtcttaattt
    17701 atatgattag gtataaaggt tttattttat tcttgttgct gatgttgatt ggatgtgagc
    17761 aacgtgaaga gttaatttct aatttatctc aaagacaggc aaatgaaata atatctgtgc
    17821 tagaacgcca taatattact gctagaaaag ttgatggagg taaacagggg atctcggtac
    17881 aagtcgaaaa ggggacattt gcatcggcag ttgatttgat gcgcatgtac gatttgccaa
    17941 atccggagag agttgatatc tcacaaatgt ttcctacaga ttcattagtg tcttctccaa
    18001 gagctgaaaa ggcccgttta tatagtgcta ttgagcaacg gctggaacag tctttagttt
    18061 ctattggtgg tgttatttcg gcaaaaatac atgttagcta tgatcttgaa gaaaaaaata
    18121 tatcttcaaa accgatgcat atatcagtaa tcgctatata tgactcaccg aaagagtctg
    18181 aactattagt tagtaatatt aagcgatttt tgaaaaacac cttttctgat gttaagtatg
    18241 aaaatatatc tgtcatatta actccgaaag aagaatatgt ttatacaaat gtacaacctg
    18301 ttaaggaaat taaatcggaa tatttaacaa atgaagtaat atatgtattt ctcgggatgg
    18361 ctgtactagt tgtcattctt ttagtatggg cattcaaaac agggtggttc aagagaaaca
    18421 aaatatgata agaatggatg gaatttataa aaaatatctt tcaataattt ttgatccagc
    18481 gttctatata aatagaaatc ggttgaattt gccttctgaa ctgttagaaa atggcgtaat
    18541 cagaagtgag attaataatc tcataattaa taaatatgat ctaaattgcg atattgaacc
    18601 tttaagcggg gtaaccgcta tgtttgttgc caactggaat ttacttccag ctgttgctta
    18661 ttttatcggt agtcaagagt ctcggttaat taatcatagt gagatggtta ttagttatta
    18721 tgggggcaaa atcagtaagc aaggggaggc cgctattcga agtggttttt ggcatcttat
    18781 tgcgtggaag gaaaatatat ctgttggtat atatgaaaga ataaacttat tatttaatcc
    18841 aattgctttg gagggaaatt atacgcctgt agaaagaaat ttgtcccgat taaatgaagg
    18901 tatgcaatat gcaaaaaggc actttaccgg tatccagaca tcatgcctat gatggtgttg
    18961 ttataaagag aattgaaaag gaattatgta aaaccataaa agatagagac actgagagta
    19021 agaagaaagc aatttgtgtt ataaaggatg ctacgaaaaa agctgaatca ctgcgtatag
    19081 atgctgtatg cgatggttat caaattggta tacagactgc ttttgagcat attattgact
    19141 atatttgtga atggaagtta aagcaaaatg aaaacagaag aaatattgag gactatataa
    19201 ctagtttgct ttcagaaaat ttacatgatg agaggatcat tagcacttta ttggagcagt
    19261 ggcttagttc gttgcgtaat acagtaacag aattaaaagt ggttttgccc aagtgtaatt
    19321 tagcactgag gaaaaagtta gaacttgatc tccataaata tcgctcagat gtcaagatta
    19381 ttttaaaata ttctgaaggg aataattata tattctgtag tggtaatcaa gtagtggagt
    19441 ttagtcctca ggatgtaata tcaggtgtta aaatagaact tgcggaaaaa ttaacaaaaa
    19501 atgataaaaa atatttcaaa gagttagcac ataagaaact gagacaaatt gcagaagact
    19561 tgttaaaaga gaatcctgtt aatgattaat caaataaatg caagcaatgc tctgcagcaa
    19621 agattaaata gtgaagaatt tgttaattta aatgagagat tatcatcttc gcaatcattt
    19681 gatgaggata taatctatga aataatgcaa tatttctcac agagtgaatt aaattccata
    19741 gataatgatg aactgcacaa taagattgaa caattattca attcacgttt tccttatctt
    19801 acagctgcac aaaaaagttc acttcttaat aaactcatag atgaaaatca atatgtcgat
    19861 ttgcatgagg gattttatgc atcattgtcc atatataaca atattgattt ctatattaag
    19921 actacaacct tcgattcatt aattagtgtc tttgaagcag ggagagaggc agatgattcg
    19981 acatggtagt aataagttga aaatatttat tttaagtata ttgctattaa cactgagtgg
    20041 gtgtgtttta aagtcatcat ctaattctga aaaagaatgg catattgttc ctgtaagtaa
    20101 ggattatttt tctattccaa atgatttatt atggtcgttt aatacaacca ataaaagtat
    20161 aaatgtttac tctaaatgta ttagtggtaa ggcggtttat agttttaatg caggtaaatt
    20221 catgggcaac tttaatgtta aggaagtaga tgggtgcttc atggatgcac aaaagatagc
    20281 tatagataaa ctattttcta tgctgaaaga cggggttgtt ttaaaaggta ataagataaa
    20341 tgataccatc cttatagaga aggatgggga agttaaatta aaattaattc gagggatata
    20401 attgtattgt gagtaaatat aaaggtctaa atacaagtaa tatgttttac atttactcta
    20461 gtggacatga accagttaac gttgagcttg taaaagataa agaacgtaac ataattgagc
    20521 tggctccagc atggaagggt ttttttttgt ccgaaatcaa aatataaaat tcagtgataa
    20581 tgtgaactat cattatcgct ttaacattaa tagttgtgca aaatttcttg ctttttggga
    20641 ctatttcagt ggtgctctag tagagcatag ccatgctgaa aaatgtattc atttctatca
    20701 tgagaatgat cttcgggata gttgtaatac cgaatcgatg cttgataagt taatgttaag
    20761 attcatcttt tcatctgacc agaatgtaag taatgcactg gctatgatac gaatgactga
    20821 gtcatatcat ttggttcttt atcttctgag aactattgag aaagaaaagg aggtaagaat
    20881 aaagagtctg acagagcatt atggtgtgtc ggaggcttat tttagatctt tatgtagaaa
    20941 agctttaggg gcaaaagtca aagaacaact gaacacttgg cggttagtaa atggtttatt
    21001 agatgtattt ttgcacaatc aaacaatcac ttctgctgcc atgaataatg ggtatgcatc
    21061 aacatcccat ttttcaaatg agataaaaac acgactaggc ttttctgcaa gagaattatc
    21121 gaatattact tttttagtga aaaaaataaa tgaaaaaatt taatattaaa tctttgactc
    21181 tcttgattgt attgttacca ttgatagtta atgctaataa tattgactct catttattag
    21241 aacaaaatga catagcgaaa tatgttgctc aaagtgatac ggtaggatct ttttttgaac
    21301 gtttttctgc gttattaaat tatcctattg ttgttagcaa acaagctgca aaaaagagaa
    21361 tatctgggga gtttgattta tctaatccag aagaaatgct tgaaaaaatg acattacttg
    21421 tgggactcat atggtataaa gatggaaatg cattatacat atatgactct ggagaattaa
    21481 taagcaaagt tattttgctg gagaatatat cacttaatta tcttattcag tatctgaagg
    21541 atgctaacct ttatgatcac agatatccta taagaggaaa cattagtgat aaaacattct
    21601 atatatctgg tcctccggca ttggttgaac tggtagctaa tacagcaact ttgcttgata
    21661 agcaggtttc ttctattggg acagataaag ttaattttgg ggttataaaa ttaaaaaata
    21721 catttgttag tgacagaact tataatatga gaggggagga tattgtcatc ccaggggtgg
    21781 ctactgttgt agaacgttta ttaaataatg gtaaggctct gagtaacaga caagccaaaa
    21841 atgatccgat gccacccttt aatattacac agaaagtatc tgaggatagt aatgactttt
    21901 catttagttc agttaccaat agttctatat tagaggatgt atcacttatt gcatatccag
    21961 aaaccaattc tatattagtc aaaggtaatg atcagcaaat acagattatt cgcgatatta
    22021 tcactcagtt agatgtagcc aaacgacata tagaattatc attatggata atcgatattg
    22081 ataaatctga attaaataac cttggcgtta attggcaagg aacggcttca tttggtgatt
    22141 cgtttggtgc atcatttaat atgagctcat cagcatcgat aagcactttg gacggcaata
    22201 agtttattgc ttcagttatg gcattaaatc agaaaaagaa agcaaatgta gtttcaaggc
    22261 cggtgatttt gacacaagag aatattccgg ctatatttga taataacagg actttttatg
    22321 tgtcattagt tggcgagcgc aattcttcgt tggagcatgt tacgtatgga acattgataa
    22381 atgttatacc tcgcttttca tctcggggac aaattgagat gtctttaacg atagaggatg
    22441 gaacaggaaa tagtcagtca aattacaata ataataatga aaatacatct gtccttccag
    22501 aagttggaag gaccaaaatc agtacaatag cgagagttcc acaaggcaaa agtttgttga
    22561 taggtggtta tacgcatgaa acgaactcta acgaaatagt atctattcct tttctctcct
    22621 ctattcctgt aatcggtaat gtgttcaagt ataaaacaag taatatcagt aatattgtaa
    22681 gggtttttct gattcaaccc cgtgagataa aggagtcaag ttattataat actgcagaat
    22741 ataagagcct catatctgaa agagaaatac aaaagactac acaaataatc ccatctgaaa
    22801 ctactttgct ggaagacgaa aaatcattgg tttcatactt aaattactaa ctataaagta
    22861 ggtgatgtat gcttgatgtt aaaaatacag gagtttttag ctctgcattc attgataagt
    22921 tgaatgcaat gacaaattca gatgatggag atgagactgc tgatgcagag cttgattctg
    22981 gcttggctaa tagcaagtat attgactcat ctgatgagat ggcttccgct ctttcgtcat
    23041 ttataaacag aagagacctt gagaaactga aaggaacaaa tagtgatagt caggaacgta
    23101 ttttagatgg ggaagaagat gaaattaatc acaagatttt tgatttaaag agaactttaa
    23161 aagataacct tcctctagat caggatttta tagacagact aaagagatat tttaaagatc
    23221 caagtgatca agtcttagca ttaagggagc ttttgaatga aaaagatctt actgctgaac
    23281 aagtcgaatc attaactaaa attattaatg agataatatc aggtagtgaa aaaagtgtta
    23341 atgctggaat aaattcagct atacaggcta aattatttgg caacaaaatg aaacttgaac
    23401 cacagctttt gcgtgcatgt tatcgtggtt ttatcatggg gaacatatca acaacagatc
    23461 agtatataga atggcttggt aattttggtt ttaatcacag acatacaatt gtgaattttg
    23521 tagagcagtc actgattgta gacatggatt ctgagaaacc gagctgtaat gcttatgagt
    23581 ttggttttgt gttatctaaa ttaattgcaa ttaagatgat tagaacttca gatgtaattt
    23641 ttatgaagaa actagaatcc tcaagcttgc taaaagatgg cagtttaagt gcagagcagc
    23701 tattgctaac tttattatat atttttcaat atccaagtga aagtgagcaa attcttactt
    23761 ctgttataga cgtatcacga gccagtcatg aggattctgt agtgtatcaa acatatctat
    23821 cttctgttaa tgaaagtcct catgatatat ttaaaagtga aagtgaaaga gaaattgcga
    23881 tcaatattct acgagagctt gtcacaagtg catacaagaa agagctttct agataacagg
    23941 agatgaaagt gatccagtct tttcttaagc aagtaagtac taagcctgaa ttaattatat
    24001 tagtgcttat ggttatgatc attgctatgt tgatcatacc attacctaca taccttgttg
    24061 attttttaat agggcttaat attgtacttg ctattctcgt ttttatggga tcattttata
    24121 ttgaaagaat attgagtttt tctacattcc cttcggtttt gttgataacg acattatttc
    24181 gtctcgcttt atcaataagt actagtcgac taattttagt tgatgcggat gcgggtaaaa
    24241 ttattactac atttggccag tttgtcattg gtgatagtct ggctgtaggt tttgttattt
    24301 tttcaatagt aactgttgtg cagttcattg ttataacaaa aggatctgaa agggttgcgg
    24361 aggtagctgc tcgcttttct cttgacggta tgccggggaa acaaatgagc attgatgcgg
    24421 atttaaaggc tggaattatt gatgccgcag gagctaaaga aagacgcagt attctggaac
    24481 gtgagagtca attgtatggt tcatttgatg gagcaatgaa gttcattaaa ggtgatgcaa
    24541 ttgctggcat cattattatt tttgtcaatt taataggagg tatttctgtt ggtatgagtc
    24601 aacatggaat gtctctatct ggtgcattat ctacttatac catactaact attggtgacg
    24661 gattggtctc tcagattcct gctctgttaa tttctataag tgcaggattt atcgttacaa
    24721 gagttaatgg tgatagcgat aatatgggac gtaacataat gtcccagata tttgggaatc
    24781 cttttgttct cattgttaca tcagctcttg ccttggcgat agggatgttg ccaggttttc
    24841 cgttttttgt ttttttcctg atagcagtta ctttgacggc tttattctat tataaaaagg
    24901 tcgtagaaaa agaaaaaagt ctgtctgagt ctgattctag tggctatact ggtacatttg
    24961 atattgataa tagccatgac tcatctttgg caatgataga aaatctggat gctattagtt
    25021 cagaaaccgt tcctttaatt ttattatttg ccgaaaataa gataagtgca aatgatatgg
    25081 aaggtcttat tgaaaggata agaagtcagt tctttattga ttatggtgtc aggcttccaa
    25141 ctattttata tagaacaagc aatgagctta aggtcgatga tattgttttg ttaataaatg
    25201 aggtgcgtgc cgatagtttt aatatatact ttgacaaagt gtgtattaca gatgaaaatg
    25261 gagatataga tgctctaggt attcctgttg tttcaacttc atataatgaa cgtgttattt
    25321 cttgggtaga cgtttcatat acagaaaatc taactaatat tgatgctaaa attaaaagtg
    25381 ctcaagatga attttatcac cagttgtcac aagctttatt aaacaacata aatgagattt
    25441 ttggtataca agaaacaaaa aatatgttgg atcagtttga aaatcggtat cctgatctat
    25501 taaaggaagt cttccgacat gtgactatac agagaatttc tgaggtatta caaagattgc
    25561 ttggagaaaa tatttctgtt cgcaatttaa aacttattat ggagtctttg gcgctttggg
    25621 ctcccagaga aaaagatgtc ataacattag ttgaacatgt ccgtgcatca ctttctaggt
    25681 atatttgtag taaaatagct gtttctggtg agattaaagt tgtgatgctt tccggatata
    25741 ttgaggatgc aataagaaaa gggataaggc aaacctctgg tggctctttc ttgaatatgg
    25801 atatagaggt ttcggatgag gtaatggaaa ctttagcaca tgctttgaga gaattgagaa
    25861 atgcaaaaaa aaatttcgtt cttttggtat cagtagatat acgtaggttt gttaaaagac
    25921 ttatagataa cagatttaag agtatactcg ttatatcgta tgctgagatt gatgaagcat
    25981 ataccattaa tgtattaaag actatttagt gaggtttaaa tatgagtaac attaatttag
    26041 ttcaattagt tagagatagt cttttcacga ttggttgtcc tccatcaatt attacagatt
    26101 tagacagcca ttcagcgatt accatttctt tggatagtat gcctgctatt aatatagctc
    26161 ttgtcaatga gcaggttatg ttatgggcta atttcgatgc accaagtgat gtaaaacttc
    26221 agtcatctgc ttataacata ttaaatttaa tgttaatgaa ctttagttat agcattaatg
    26281 agctggtaga gcttcatcgt tctgatgagt acttacagtt aagagttgtt attaaagatg
    26341 actacgttca cgatgggatt gtttttgcag agattctgca tgagttttat cagaggatgg
    26401 aaatcttaaa tgaggtctta taatcaatga gctatataaa attgctcact caattatctt
    26461 ttcctaatag aatctcgggg ccaatcttgg aaacaagtct tagcgatgtt tcgattggtg
    26521 agatttgtaa cattcaggct ggaattgaaa gtaatgaaat tattgcaaga gctcaggttg
    26581 taggatttca tgatgaaaaa acaatattaa gcttgattgg aaattctcgt ggactttcac
    26641 ggcaaacgtt gattaagccc actgcccagt ttcttcatac gcaagtcggc cgtggattat
    26701 tgggagcagt agtcaatcct ttaggggagg ttactgataa gtttgctgtt acggataaca
    26761 gtgaaattct ttatcgacct gtagataatg ctcctccgct atatagtgaa agggctgcaa
    26821 ttgagaagcc ttttttaaca ggtgttaagg ttattgattc tttactcacg tgtggtgaag
    26881 gacagcgaat ggggattttt gcgtcagctg gttgtggcaa aacttttctc atgaatatgc
    26941 tcattgaaca tagtggtgct gatatatatg ttattgggtt aattggtgag cgaggtcgag
    27001 aggttacaga aacggttgat tatttgaaaa actctgagaa aaaaagcagg tgtgttttag
    27061 tatatgcaac ttcggattac tcttcggttg atcgttgtaa tgctgcatat atagccactg
    27121 ctatagccga attttttagg actgaaggac ataaagtagc gctttttatt gattcattaa
    27181 caaggtatgc cagagcatta cgtgatgtgg ccttagccgc tggagaatca cctgccagaa
    27241 gaggctatcc ggtttcggtt tttgatagct tacccagact tcttgaaagg ccaggaaagt
    27301 taaaggcagg tggctctatt actgcatttt acactgttct tttggaggat gatgattttg
    27361 ctgatccatt agctgaagag gtaagatcca ttttagatgg acatatatat ttgagcagaa
    27421 atctagccca aaaaggacaa tttcctgcaa ttgattcctt aaaaagtata agcagggtat
    27481 ttacacaggt tgttgatgaa aaacatcgta ttatggccgc tgcatttcgg gagctacttt
    27541 ctgaaataga agagctaagg acaattattg actttggtga atacaaaccg ggggagaatg
    27601 cctctcagga taaaatctac aacaaaatat ctgttgttga gagttttctg aagcaagatt
    27661 atcgactcgg ttttacttat gagcagacaa tggagcttat tggtgaaaca attagataag
    27721 gttttaaaaa tcaaagataa atatcaacgg tctgttaaat taattgaagc acacatatta
    27781 acactaaaaa aaaaactctt tgtatcgaga tgtggaggca ttagataaaa ggattatata
    27841 cttcctgcaa ctggagaatg acttggaacc tgtaggcgct cagagtgtta gtcagctttt
    27901 caatacaaga cgtaagatcg ctatagtaaa aaagcatatt atccaatatc agtctgaacg
    27961 aattcttcta aagggaagaa ttgaagaaat tcaaaaagat attgatgagg caaatgcttc
    28021 taaaagaaaa ctgcttcata aagagagtaa aatatgtaaa cggattggct tgattaaaag
    28081 aaataacttt gccaaacagt tgatacttga tgagttatca caagaggata tgaagtatgg
    28141 cattagataa tataaaccta aattttagta gtgacaaaca gatagaaaaa tgtgagaaac
    28201 tatcttcaat agataatatt gatagtctcg ttttgaagaa gaagaggaag gttgaaattc
    28261 ctgagtactc tttaattgca tctaactatt ttactattga taagcacttt gaacataagc
    28321 atgataaagg agaaatttat agtggcatta aaaatgcgtt cgaacttaga aacgaacgag
    28381 cgacatattc tgatattcca gaatcaatgg ccattaaaga aaatattttg ataccagatc
    28441 aagatatcaa agcaagggaa aaaataaata tcggcgatat gagggggatc ttttcatata
    28501 ataagagtgg aaatgcagac aagaacttcg aaagaagtca tacttcttct gtaaaccctg
    28561 ataatctgct agaatctgat aatagaaatg gtcaaattgg tttaaaaaat catagcttgt
    28621 ctattgataa gaatattgct gacatcattt ctttactaaa tggaagtgtt gctaaatcat
    28681 ttgagctgcc tgtaatgaat aaaaatactg cagacataac cccatccatg tcattgcaag
    28741 aaaaatcaat agttgaaaat gataaaaatg tttttcaaaa aaatagtgaa atgacttacc
    28801 actttaaaca gtggggggct ggacattctg ttagtatttc agtggagtct ggttcttttg
    28861 ttctaaaacc gtcagatcaa tttgtaggaa ataaacttga cttaattttg aaacaagatg
    28921 ctgagggtaa ttacagattt gatagcaatc aacataataa ggggaataaa aataatagta
    28981 caggatataa tgaacagagt gaagaagaat gctaagaatt aaacattttg acgctaacga
    29041 aaaactacag attttatatg caaagcaact ctgcgagcgt ttttcgattc agacattcaa
    29101 aaataaattt acaggcagtg aaagtttagt cactcttact tccgtgtgtg gggattgggt
    29161 aattcgtatt gatacattat cttttttgaa aaaaaaatac gaggtatttt caggattttc
    29221 tacacaagaa tctttactgc atttatcaaa atgtgtcttt atagagtcgt catctgtatt
    29281 ttcgattcca gaactgtctg ataagattac tttccggatc acgaatgaaa tccagtatgc
    29341 aactactggg agtcatttat gctgtttttc ctcttcttta ggtattattt attttgacaa
    29401 gatgccggta ttacgtaatc aagtttctct tgacttattg catcatcttt tagagttttg
    29461 cttaggttca tctaatgtaa ggctggctac tttaaaaaga attcgtactg gtgatataat
    29521 catagttcag aaactttata atttattatt gtgtaatcaa gttattattg gggattatat
    29581 tgtgaatgat aataatgagg caaaaattaa tctgtcagaa agtaatggtg agtcagatca
    29641 cacagaagtt tctttggcat tattcaatta tgatgatatc aatgtaaaag tggactttat
    29701 ccttttagaa aaaaagatga caatcaatga actaaaaatg tatgtagaaa acgaattatt
    29761 caagtttccc gatgacatag ttaaacatgt aaatattaaa gtaaatggtt ctttggttgg
    29821 gcatggggaa cttgtttcta ttgaggatgg ttatggtatc gagattagtt cttggatggt
    29881 aaaggagtaa tctcatgctg agtgatatgt ccctcatcgc tacactttct ttttttacgc
    29941 tattaccatt tttagttgct gctggtactt gttatataaa gttttctatt gtttttgtaa
    30001 tggtacgaaa tgcacttggt ttgcagcagg ttccatcaaa tatgactctc aatggtattg
    30061 cattgattat ggctttgttc gtgatgaaac ccattattga agctggttat gaaaattatc
    30121 taaatggccc gcaaaaattt gatacaatat cagatattgt ccgtttttct gattctggct
    30181 tgattgaata caagcaatac ttgaaaaagc atacagatct tgagttggca cgttttttcc
    30241 aaaggtctga agaagaaaat gcagatttaa agagtgcaga gaataatgat tattcattgt
    30301 tttccctctt gcctgcatat gcgctaagtg aaattaaaga tgcgttcaaa ataggttttt
    30361 atttatattt gccatttatt gttgttgatc ttgttatttc aagcatttta cttgcacttg
    30421 gtatgatgat gatgagtcca atcacaatat cagtaccaat aaaacttgta ttatttgttg
    30481 cgcttgatgg gtgggggatc ctgtctaagg ccttgattga acaatatatc aatattcctg
    30541 cttagatgat ggaggtaatg tgtctgatat agtttatatg ggtaataagg ctctttattt
    30601 aatccttatc ttttccttat ggccagtagg tatagctacg gttattggat taactattgg
    30661 tttattacag acagtgactc aacttcaaga gcagacactt ccttttggta taaagcttat
    30721 aggtgtctca atatctttgc tacttctttc tggatggtat ggtgaggttt tattgtcttt
    30781 ttgtcatgaa ataatgtttt taattaagag tggggtttga tggacatttc aagctggttc
    30841 gaaagtattc atgtgttttt aatactcctg aacggcgttt tttttagatt ggctccatta
    30901 tttttctttc ttccattttt aaataacggt ataatttctc catctattag aatacctgtg
    30961 atttttcttg ttgcatcagg attaattact tctggtaagg tagacatagg ttcttctgtt
    31021 tttgaacatg tttatttcct tatgttcaag gaaataattg ttggtctcct tctctctttt
    31081 tgcttgtctc ttcccttttg gatatttcat gctgttggta gcattattga caaccagcgt
    31141 ggggcaacgc ttagtagttc aattgatcct gccaatggtg ttgatacgtc tgagttggca
    31201 aaatttttca atcttttttc tgcagttgta tttctataca gtggtggaat ggtctttatt
    31261 ttagaatcca tacaattgtc ttataatata tgcccgttat tttctcaatg ttctttccgt
    31321 gtctcaaata tattaacatt tctgacttta ttggcaagtc aggctgttat tttagccagt
    31381 cctgttatga tagtattgtt actatcagaa gtattacttg gtgtattatc gagatttgct
    31441 ccgcagatga atgctttttc cgtatcatta actattaaaa gtttacttgc aatatttatt
    31501 attttcatct gttcttctac tatttacttt tctaaagttc aatttttcct cggtgaacat
    31561 aaatttttca caaatctatt tgttagataa aatattatgg caaataaaac agaaaagccg
    31621 acacctaaaa aactaaagga tgccgcaaaa aaaggacagt catttaaatt taaggattta
    31681 acgacagttg ttattattct ggtagggaca tttactataa tatcattctt ttccttaagt
    31741 gatgtaatgc tcttatacag atatgtaata attaatgact tcgaaattaa tgagggtaaa
    31801 tacttttttg cagtggttat tgtctttttt aagataattg gcttcccact ttttttctgt
    31861 gttctttcgg ctgtgttgcc aacattggtt caaacaaagt ttgttcttgc gactaaagct
    31921 atcaagattg atttttcagt attaaaccct gttaaagggt taaaaaaaat atttagtata
    31981 aagacaataa aagaattttt caaaagcatt ctgcttctta ttattctagc attaacaacc
    32041 tatttctttt ggattaatga ccgaaaaata attttttctc aggtgttttc tagtgttgat
    32101 ggcttatatc ttatttgggg ggggctgttt aaggatataa tattattttt cttagcattt
    32161 tctatttttg ttattattct tgactttgtg attgagttca ttttatacat gaaagatatg
    32221 atgatggata aacaggagat aaaaagagaa tatatagagc aagagggaca ctttgagaca
    32281 aagtcgagaa ggcgtgagtt gcatatcgag attctttcag agcagactaa atctgatata
    32341 cgtaattcaa aattagtggt aatgaacccg actcatattg caattggtat ttatttcaat
    32401 ccagaaatag cgcctgcacc ttttatttct ctcattgaaa ctaaccagtg tgccttggct
    32461 gtcagaaaat atgcaaatga agttggtata ccaactgtgc gtgatgtgaa attagctaga
    32521 aaactatata aaacacatac aaaatatagt tttgttgatt ttgaacactt ggatgaagtc
    32581 ctacgtctta ttgtttggct tgagcaggtt gaaaacactc attaataagt aaaggagatg
    32641 tgttatatgg gagttaattt ctgtaataaa ataggtattg atcagtctga atttgaaata
    32701 gaatcttcca tcattaactc cattgctaat gaggtattga acccaatatc tttccttagc
    32761 aacaaggata taataaatgt tttactcaga aagatttcat ctgaatgtga cctggttaga
    32821 aaagacattt atcgttgtgc tcttgagttg gtcgttgaaa aaacacctga tgatttataa
    32881 tcattcataa taactctttt gctgatataa atatttttgt tgaagatatt tatgtagtca
    32941 caggatcaat tgtagaaaat tgttatgttt ttgttaggaa ctgaactaaa taatatatga
    33001 taagacagca aaaacgactg accataatcc ttcttttatt aggagttgat aagagagact
    33061 attcatcctg taatgttaaa actctgttat atagtattcg cgattatgca aagtctgtaa
    33121 atgataatga gatactaacc gagagtaatc gtttattatc tcattgcatt tcagatagta
    33181 acggagcttt ctttaaaagt agtaaatacg ttccccttaa gtacttacgt aaaaggagaa
    33241 tagcacgaaa gataccaaat gattagcact ttgctagtaa gcttgctggt aaactatatt
    33301 gaccgttggg tttgttttga gactgtaatt taaattgtgt aattgcctgt ttttgatatg
    33361 tttactccaa caacggagac aggtaaatta tggacgaaaa gaagcttaaa gcccttgcgg
    33421 ctgaactggc taaaggcctc aaaactgagg ccgacctcaa tcagttttcc cgtatgctga
    33481 cgaaatatcc cggctcttaa aacgatagtg gctcaggctt tcatttttca gcgttcccca
    33541 gaag
//
LOCUS       DAJTNA010000023        32593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTNA010000023 DAJTNA010000000
VERSION     DAJTNA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32593
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.023"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            522..761
                     /locus_tag="OO161_000947"
     CDS             522..761
                     /locus_tag="OO161_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5462108.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(949..1926)
                     /gene="intI2"
                     /locus_tag="OO161_000948"
                     /pseudo
     CDS             complement(949..1926)
                     /gene="intI2"
                     /locus_tag="OO161_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2258..2731
                     /locus_tag="OO161_000949"
     CDS             2258..2731
                     /locus_tag="OO161_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5462109.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2826..3351
                     /gene="sat2"
                     /locus_tag="OO161_000950"
                     /pseudo
     CDS             2826..3351
                     /gene="sat2"
                     /locus_tag="OO161_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
     gene            3420..3917
                     /locus_tag="OO161_000951"
     CDS             3420..3917
                     /locus_tag="OO161_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462110.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3978..4349
                     /locus_tag="OO161_000952"
     CDS             3978..4349
                     /locus_tag="OO161_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462111.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4759..5136
                     /locus_tag="OO161_000953"
     CDS             4759..5136
                     /locus_tag="OO161_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462112.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5159..5311
                     /locus_tag="OO161_000954"
                     /pseudo
     CDS             5159..5311
                     /locus_tag="OO161_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5367..6983)
                     /locus_tag="OO161_000955"
     CDS             complement(5367..6983)
                     /locus_tag="OO161_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5462113.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(6984..8510)
                     /locus_tag="OO161_000956"
     CDS             complement(6984..8510)
                     /locus_tag="OO161_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5462114.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8513..10180)
                     /locus_tag="OO161_000957"
     CDS             complement(8513..10180)
                     /locus_tag="OO161_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5462115.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10177..12285)
                     /locus_tag="OO161_000958"
     CDS             complement(10177..12285)
                     /locus_tag="OO161_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5462116.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPLLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12272..13093)
                     /locus_tag="OO161_000959"
     CDS             complement(12272..13093)
                     /locus_tag="OO161_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5462117.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13253..15082)
                     /gene="glmS"
                     /locus_tag="OO161_000960"
     CDS             complement(13253..15082)
                     /gene="glmS"
                     /locus_tag="OO161_000960"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5462118.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRIGHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTELLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15244..16614)
                     /gene="glmU"
                     /locus_tag="OO161_000961"
     CDS             complement(15244..16614)
                     /gene="glmU"
                     /locus_tag="OO161_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5462119.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILMF
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(16964..17383)
                     /gene="atpC"
                     /locus_tag="OO161_000962"
     CDS             complement(16964..17383)
                     /gene="atpC"
                     /locus_tag="OO161_000962"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5462120.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17404..18786)
                     /gene="atpD"
                     /locus_tag="OO161_000963"
     CDS             complement(17404..18786)
                     /gene="atpD"
                     /locus_tag="OO161_000963"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5462121.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18813..19676)
                     /gene="atpG"
                     /locus_tag="OO161_000964"
     CDS             complement(18813..19676)
                     /gene="atpG"
                     /locus_tag="OO161_000964"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5462122.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19727..21268)
                     /gene="atpA"
                     /locus_tag="OO161_000965"
     CDS             complement(19727..21268)
                     /gene="atpA"
                     /locus_tag="OO161_000965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5462123.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21281..21814)
                     /gene="atpH"
                     /locus_tag="OO161_000966"
     CDS             complement(21281..21814)
                     /gene="atpH"
                     /locus_tag="OO161_000966"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5462124.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21829..22299)
                     /gene="atpF"
                     /locus_tag="OO161_000967"
     CDS             complement(21829..22299)
                     /gene="atpF"
                     /locus_tag="OO161_000967"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5462125.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAYKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22361..22600)
                     /gene="atpE"
                     /locus_tag="OO161_000968"
     CDS             complement(22361..22600)
                     /gene="atpE"
                     /locus_tag="OO161_000968"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5462126.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22647..23462)
                     /gene="atpB"
                     /locus_tag="OO161_000969"
     CDS             complement(22647..23462)
                     /gene="atpB"
                     /locus_tag="OO161_000969"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5462127.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23471..23851)
                     /gene="atpI"
                     /locus_tag="OO161_000970"
     CDS             complement(23471..23851)
                     /gene="atpI"
                     /locus_tag="OO161_000970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5462128.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(24468..25091)
                     /gene="rsmG"
                     /locus_tag="OO161_000971"
     CDS             complement(24468..25091)
                     /gene="rsmG"
                     /locus_tag="OO161_000971"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5462129.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQRPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(25155..27044)
                     /gene="mnmG"
                     /locus_tag="OO161_000972"
     CDS             complement(25155..27044)
                     /gene="mnmG"
                     /locus_tag="OO161_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5462130.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALDGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSHRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(27423..27866)
                     /gene="mioC"
                     /locus_tag="OO161_000973"
     CDS             complement(27423..27866)
                     /gene="mioC"
                     /locus_tag="OO161_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5462131.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWVNLLK"
     gene            complement(27956..28414)
                     /gene="asnC"
                     /locus_tag="OO161_000974"
     CDS             complement(27956..28414)
                     /gene="asnC"
                     /locus_tag="OO161_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5462132.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            28566..29558
                     /gene="asnA"
                     /locus_tag="OO161_000975"
     CDS             28566..29558
                     /gene="asnA"
                     /locus_tag="OO161_000975"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5462133.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(29563..31014)
                     /gene="viaA"
                     /locus_tag="OO161_000976"
     CDS             complement(29563..31014)
                     /gene="viaA"
                     /locus_tag="OO161_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5462134.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEKPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQLFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(31008..32504)
                     /gene="ravA"
                     /locus_tag="OO161_000977"
     CDS             complement(31008..32504)
                     /gene="ravA"
                     /locus_tag="OO161_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5462135.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNCIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         8311 a         8233 c         7560 g         8489 t
ORIGIN      
        1 ggtcggcagc cctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt
       61 tttaaggatt atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag
      121 ctaatacgaa aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttaa
      181 ctaatctagc taacctgccg ctcagcttag tacgatccta tatccaactg atcataatcc
      241 tttcatcagt atcccaaatt aaatccccct cctagactgc ctcaccaaca ctttacagtg
      301 atcccgtatt tccggacagg tgattgattt ttttcgcttg tacaggtgtt aagccacagt
      361 tatcttgatg tggacattat gcattgtaat agcccattaa catgcgctaa gagcataaat
      421 taatatacag taacaagcat tacgaggtgc taatgtttgg aaagcagcct cttccaacta
      481 caataataag ttaactatcc tccacccaca aataattaca aatgacccct ttccgctata
      541 actccgatct caccagcggc tcgctacaaa cccgagagtg tcgcattatt actggcttgt
      601 tactccaaga gcttgatgaa gctgcctggg ataaagccat gtataaggaa aatgtgcttc
      661 agaaacgcac gcaatctacg gtaagacgta tttcttcagc gcttagaaaa cgcctagaac
      721 acctaagctc tgacttttgg gcttttgcgt ttttatgtta ggcattgtct ggcaactgga
      781 ggaagtgtaa tgaccatgaa tggtcactgt gattgacagt atcttgctta aacttatctg
      841 aacaatggta gaactggctc atatttatcc cctctatgat tgtttatctt tttcaccata
      901 aaaagtatca ggggctactc agggctacat aaagatattt atctactttt actgattgat
      961 aagtagcatc agtccatccg caggactggt ggtgccggca aaatgctgac ccaacacatg
     1021 cgtatagatt tgcgtggtct taacatcgtt atgccctaag agttcttgca cagtgcgaat
     1081 atcacgcccc gcttgtaata gatgcgtagc aaacgagtga cgaaatgtat gacaagtgac
     1141 acgcttgcta acgatgcctg ctttttgtac ggctgccttc aatgcctttc gcgcaacgga
     1201 gtcatgcaga tgatggcggc ataatttgcc gttatacggg tggttgcaga gcgtgctgga
     1261 gggaaagaca aacatccacg ccgcttgtcg ataagcagaa gggtatttgt gatctaaagc
     1321 aaaaggcagc gatggcccta cgccttgtaa gttgtcgtct tgctgaataa gccgcgcttg
     1381 ctcaatgagt tattttattg ctgggattag gcgcgtgggc agtaggctgt ttctgctttt
     1441 cccaccctta ccgtcatgca cagtgatgca gccattatca aaatcaaaat ctttaacccg
     1501 caaacgcaag cattcattaa tgcgcaaacc tgcaccatac agcagcgtaa aaataacttg
     1561 gttgcgagta tccataacct gcaaaatgcg ttgcacttca tttgcagaga taacagaggg
     1621 tagccgtcta ggcttgcttg cagggatata atcaatatcg cccaacggct gttgtaaaaa
     1681 cctgttgtac aaaaaagcta gggcatttaa agcgattttc tgcgtgttta tggctacatg
     1741 tctgctgttt gctaagctgg ataaaaacag cctgacctct tcactgccca tggtctgagg
     1801 atgacgtttt ttgtgaaaca gaataaaacg cttaatccag tgcaggtaag ttttttcagt
     1861 tttcagcgca taaccttttt gtcgcatatc cgtgcgtata gaatttaaaa atggactgtt
     1921 agacataaaa cgctccttgt cttgcaactg tctgcctata cagcctattc tagctgggat
     1981 ttaaaaaagt gcctgttttt tacgcctaga gatgcttgtt taccggtaga gttttaattt
     2041 aatgctaaat aaattaaaat gttatgagtt ctttgggtga gataatgtgc atcgtgcaag
     2101 caggatagac ggcatgcacg atttgtaata acagagtgtc ttgtattttt aaagaaagtc
     2161 tatttaatac aagtgattat attaattaac ggtaagcatc agcgggtgac aaaacgagca
     2221 tgcttactaa taaaatgtta acctctgagg aagaattgtg aaactatcac taatggtagc
     2281 tatatcgaag aatggagtta tcgggaatgg ccctgatatt ccatggagtg ccaaaggtga
     2341 acagctcctg tttaaagcta ttacctataa ccaatggctg ttggttggac gcaagacttt
     2401 tgaatcaatg ggagcattac ccaaccgaaa gtatgcggtc gtaacacgtt caagttttac
     2461 atctgacaat gagaacgtat tgatctttcc atcaattaaa gatgctttaa ccaacctaaa
     2521 gaaaataacg gatcatgtca ttgtttcagg tggtggggag atatacaaaa gcctgatcga
     2581 tcaagtagat acactacata tatctacaat agacatcgag ccggaaggtg atgtttactt
     2641 tcctgaaatc cccagcaatt ttaggccagt ttttacccaa gacttcgcct ctaacataaa
     2701 ttatagttac caaatctggc aaaagggtta acaagtggca gcaacggatt cgcaaacctg
     2761 tcacgccttt tgtaccaaaa gccgcgccag gtttgcgatc cgctgtgcca ggcgttaggc
     2821 gtcatatgaa gatttcggtg atccctgagc aggtggcgga aacattggat gctgagaacc
     2881 atttcattgt tcgtgaagtg ttcgatgtgc acctatccga ccaaggcttt gaactatcta
     2941 ccagaagtgt gagcccctac cggaaggatt acatctcgga tgatgactct gatgaagact
     3001 ctgcttgcta tggcgcattc atcgaccaag agcttgtcgg gaagattgaa ctcaatctca
     3061 acatggaacg atctagcctc tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa
     3121 ggagtcgcgc acagtctcat cgaatttgcg aaaaagtggg cactaagcag acagctcctt
     3181 ggcatacgat tagagacaca aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt
     3241 ggctttactc tcggcggcat tgacctgttc acgtataaaa ctagacctca agtctcgaac
     3301 gaaacagcga tgtactggta ctggttctcg ggagcacagg atgacgccta acaattcatt
     3361 caagccgaca ccgcttcgcg gcgcggctta attcaggagt tagagagctg gggaagacta
     3421 tgcgcgatct gttgaaggtg gttctaagcc tcgtacttgc gatggcatcg gggcaggcac
     3481 ttgctgacct gccaattgtt ttagtggatg aagctcgtct tccctatgac tactccccat
     3541 ccaactacga catttctcca agcaactacg acaactccat aagcaattac gacaatagtc
     3601 catcaaatta cgacaactct gagagcaact acgataatag ttcatccaat tacgacaata
     3661 gtcgcaacgg aaatcgtagg cttatatata gcgcaaatgg gtctcgcact ttcgccggct
     3721 actacgtcat tgccaacaat gggacaacga acttcttttc cacatctggc aaaaggatgt
     3781 tctacacccc aaaagggggg cgcggcgtct atggcggcaa agatgggagc ttctgcgggg
     3841 cattggtcgt cataaatggc caattttcgc ttgccctgac agataacggc ctgaagatca
     3901 tgtatctaag caactagcct gctctctaat aaaatgttag gcctcaacat ctagtcgcaa
     3961 gctgagggga accactagtg tcatacgaac ctccaagaga cggttacaca aacgggtaca
     4021 ttgttgatgt catgtatgac aatcgcccaa gtaagtatcc agctgtgttc agaacgtacg
     4081 tccgaattcc agactatgtc gaactagata acgcggagga gctggccgac gcaatcattc
     4141 gagaggttga gttaaaggag gcatggctca caagggtctt ggggaactca gccgatcctg
     4201 acgcttctgt gggcaagtgg gaccggcgcc tagcgcagtg cggtggtatt gatgagatgt
     4261 caagtggggt gcaggcaaca tacttcagtc caaacggtca gctggtcggc ttagtggcat
     4321 acattgatgc cgaatggatg gatgaatagc tctgacgtta cgattcaggt gagacaacca
     4381 gatgggcatt gcgtacaagc tcagttgcca ccgatgtggc tacgagtctg atcttcttta
     4441 cctaggccaa ggtatggcga tgcttcctga gcaaatagtc ggaacgtgca catgctgcga
     4501 caatctaacg acgatttcta taaatgaaag ctacggagta tgctcacttt gtggggctaa
     4561 ggatcgtgtg atatttacaa gctatcagca tgaggcaagg ccaagaaatc cctgctatct
     4621 agagagaagt ttcaaatatc aatgcccaag atgccatgcc ttcagtatgg agcctccgga
     4681 tctaccggag atgctttggg actaatgctc cgctttgcct cgggtatccg ttcgacctat
     4741 gtttaagagg aaacgcacgt gtctgccgcc aaagctcttg cctatgcact ttgtgacaag
     4801 ttggaaagca aaaaagttga tgaagtcgta cgaaatcttg tttctagcgg ctggaatatt
     4861 agggaagtgg cctgggaagc ttcgtccttt gatcatgaaa tgccctactt tgacacgggg
     4921 cgggacttcg atattggatt tcctgacacg ctgtcatcca agattgagtt tttgaccgcg
     4981 aatggatcaa tcgtccaagc acgtgctcgc ctgcacttca agcaggctct aatttttgcc
     5041 aaggcgtcca aacatgtcgc cgcgctcaag aactcattgg attactacta tggatcgtct
     5101 gtaatccccc ctcaaaacca cggaggctat ttgtagaaaa ttccattaat atgaactcaa
     5161 ggtgttccct acggacgaat cagcgatgaa ggtggtgtac ctggctatcc aggcggcgac
     5221 caagaaatgg acgatgccga tccgcaactg gaagccggcc atgaaccgtt ttatgatcga
     5281 atttggtgac cgtttaaatg gccaccttta actgaccggc aaaagcactt acacaaaagg
     5341 gtttacaggc tctttcagtc attcctttaa tgcgtaaatt gctctctaac cactctctca
     5401 gcccaccctt ccatatcctc tctcgatact tgcagctccc ccaacccctt tggatgattc
     5461 acccctctat gcccgtcttg tccatataat tgatcaaaca aactatttgg ccaattcaat
     5521 gagctcttca ccactccacg ccgtatcttt tcaaaatcat tccgccatgt ttcgctatca
     5581 acgccactca acactttggt agaaagcatt ttcaccccat ccgatgcatc cacctctagc
     5641 aatacaaatt cactgccatt acgttgcacc ccaacggctt taatcactct tggtgagcca
     5701 tctttacaca gatgtaatcg gctacgccca acttttggca aaaccaaggt ttcttcaaaa
     5761 agcacccgac atgcaaattt agtttttaga attgaaagta gctcatcaaa agcagcaaat
     5821 cgattagcaa aaatagagtt gtaattggtt gcatcctgct tcccacccac atctgccgct
     5881 gctaggacac cacctaagtg tggctcatca gtactaacca aattactatt ttcacttgtt
     5941 gttgcttctt ctttttctaa aatagtttgg ctactttttc ttgccctgcg agatttttca
     6001 attcgactag gtctactaaa acttatccat gtcggctcag cctctatgac tcgtgtgtct
     6061 gtgtctatat tggctgtttc ctcatcgtcc aactgtagat gctcatcatc aacaactgac
     6121 tctgttgaaa ccgctatgtg ctgcgtacta ccatcacctg cctccttttc ctgaaaagag
     6181 gcatggctaa tagctgttgt gctaggaagc atagcgttga tttctaaacc tactatttcc
     6241 tcaactaaat aatccttatc ctcgttagaa gaacgtcctt ttacatgtaa tttccaaccc
     6301 tgcatgggcg gagggtcaaa gctaaagcac cagctttcaa cgccgttctt aatttctcta
     6361 ttttgttgat agtgcctaaa aatactttca tacgaatcca taacatcttg atccgaaaac
     6421 aaccaaacca aaagctgcac tacggccgac tgctctaacg cccctttagg aaacgatgaa
     6481 ctgggtaaga tccttatctc taaatgatct cgctcaacct catactgaac gtcgaactct
     6541 tgctgtaaag cggtactgct caaacagctt cgacagaaat aggagttaat taaaaataat
     6601 gaccgagcta gctctaactg tgggatgtga taacgagtct tgctatatgg tgtttcagct
     6661 tcgtaaataa atgaactctg cgctccatcc ttgttgcgta cggaagcaaa ggacgagaga
     6721 ttaggaaaat cgattatttt ggctcgttga agatccgatg cttcaaactc aatcacacga
     6781 tccgccttat ttatttgctt tgttgtactg ttaaccacct tagctcgact taataaaggc
     6841 aaatgagtaa atcgagtcca tttccgttct tgcattggat taaaccaaac aaaaatacgc
     6901 cactccttat gacccgagtt acggaataaa tgaccaatat gtacaacctg cacattgtca
     6961 ttaaatgtag ctagcctaac cactcactct tccccataaa ccatttccaa gaatctttgt
     7021 gcttcctcag ttatccgctc tttagataac gttgcacttc ttaataatcg ccagcgccta
     7081 agctcaacat cttcctgttt aagcttaata aaagcttggc taatccgtct aatttgataa
     7141 tcttccacac tctctgagta acgctttaag caaagcgcta ccaaaggcag tttctgtaga
     7201 ttttttgcaa gagaggttcc gttaggagtt tgctttaaca gccagctcga tgtcgctctt
     7261 gggtgatcaa ggctactatc tagacgcttt atgattctta atagttgtcg tacagcaatt
     7321 cgatctcttt ggttccaatc aactctaggg gcgggtgcca gacgctcttg ttgatgctgt
     7381 tgattccagt gaactagcca atccctgtca tgtcggtaaa gccaagcgta tagcacccca
     7441 ccctctaaag actgtcttgc cgccttaatt ccttggtatt tatgcactag ttgctgccag
     7501 tctttatgct taacagataa atcttcagag ttaggttgca cagactggct aacaggtctt
     7561 gtcgttatag agtgctcagt aagagcactt gcctgctgta gcgcttctat aaccgttagt
     7621 tttggcaata aggcttgcca cacaatacta tgctgtaaat aactaaaggc ttttctatgc
     7681 ttacggaata tacttttcag ccaacacgtg tccttgttct ctgccaactt taaatccagt
     7741 ttctctagtg cctcatcact aaaagtctgc ctcactctct ccgccaccaa gtcatgacga
     7801 atgtgcttgc ttttggttag ccctagatcc tgcgctaagc gctgataaaa cagcgtccac
     7861 tgctcaaggc ttggggaaag ctcttgcgct cgtggagcat ctaacagagg ggctatataa
     7921 gcagctagtg ctgttaattg agatagggag tctttggggt agtctgaaag cagctcagta
     7981 tgacccaaag cccaaaattg atgtcggtga tcatctacag ctctatcaaa gaagactaaa
     8041 gcaccgtgtt ttggacaata tggcaaagcg ggcaaatacc aatctcgttg ccaaaaggct
     8101 tccccatacc tatttagctg aagagcaacg caatcagggc agtatctaaa gcggttatcg
     8161 ctcttaactc tagaagcagc gactcctagc attaaatgca ccgcaccttg cgcttggtac
     8221 tccattaacc gaatagcttc gtctcggcgc tccttgccta caaacggagc atataaaggg
     8281 aataaggtat gctcataaat aagctgctga acagcgtaac gtcctgtttg atgtagatgt
     8341 cttgctatca cacctaaatg cgagggcaga cctaaggtag cgaccacctt gcggttgcca
     8401 tacacctcat ccaacagctg cttaggacta acaatccctt gataaacgcc tgcccgtgca
     8461 atagtgctat aaatcagctc attcgagtac ggaacaggaa agtttctcat ggctaacctg
     8521 cttgtttaaa taagctcgcc atatccacta ttaccccttt cccttttaac cgttcatgca
     8581 tggttttttc aggttggcgt tgtgaataga tataacgtaa atccgtatca ggcaagctat
     8641 cccagtcgct gggcttgact acctttatag ccgaaacctt tttactcttg gagggctttt
     8701 ctagttctga cactaccgtt tcgccttcca tcaaccactg caaaacaaga ggcagtaact
     8761 tttgtcttgt catcgttgga ttctggctaa acgctttttt aatagtggga attaacaggc
     8821 ttgaatcgta atcctctttc agcatcagat ataaatgacg ctgatcttcg gtatctaact
     8881 cttgaagggc tttttcttct ggtgtttgtt cttgtatcgc tgcgatatct agctgaagtt
     8941 ggattaaccg tttatcaatc tcgggaacga ctagatcaga ataacgagca atgcgttctg
     9001 ggatacccga gcgtaatgcc tctagcatgg ggtgcacagg ctttaactca tcttgataca
     9061 cttgccgcaa taaaccagcg gtaatacgct cattgcctaa agctagcgca cggagctgag
     9121 cgagtacaaa aagttttact acaatgtcca tcactccttg gcttagctca taccacacat
     9181 cacggacctc atccgataac agcgcatctt tgcgttgtaa aagctgtaat tgccaaagat
     9241 tatccgtaaa agcgatccac tcttgattgg gctttccacg ttgcgtttgt tgtataggat
     9301 cccagaatat agctccaaac cctgcccctc tacgtgcaga ccgcaaatca gcctcaaaaa
     9361 tctctcgtgc tttaggggta ccaatcaaca tcactggtac gccaataata ttcaccatcg
     9421 tcacaaaaaa gttcagcatc tcttgagatc cacccgaacg agagcggctt aaatgctgaa
     9481 tttcatcaat aaccaacaac cctaaagcat gtgcattggc tatttgcgac atcaaagcca
     9541 acatggtttc tataccatga cgttttaagc cataacgacg ctcatagttc gagcccaagg
     9601 ctcgatccaa cgctctgaaa aaattcaagc agatttcttt tagcgaacca ttatgcgagc
     9661 agtctatttt caaatacacc acctgctcta cattgagttc acgatggtaa atcacctgag
     9721 gatacgtggc tagaatacga tgaagagagg tcgtcttccc actaccagaa caaccaatta
     9781 acaataagct ttgtgccgta gatcgtgcct cctcaaagcg aaatgtctcc aactctcccg
     9841 tttgaacacg ctcataacca ttttgtaaat gcttttgtaa atctcctgtt ttaggatttc
     9901 tgcctacgta gccacctcga atcatgaccg aaatacgctc acttagtagc aaatgcgtac
     9961 ctaatggctg aaaatagtca tctggaatac gacaaatggt atgagctctg ataacacggg
    10021 acttttgcaa gtcagaggaa gtaagctgta aagaggattt cagtgatgca gcactattca
    10081 ctgactcttg taatggtggt aaggcctcga taaaaggatt atcacgataa gcctctaccc
    10141 ccgtatcacg ataaactgct tgaatccggg tagcactcat gactcatcct tttctggtgg
    10201 atcctgaaat aattcaggca cgtatgtggg taggctgtaa tcttcttgat catccgcttc
    10261 cactgcgttg aaaggaataa ctttagcctc atcacctgaa gagcttggct tcaaatgctc
    10321 cgcacgtttt ttacgctccg aggtcacggc ttctttttta ttagttttaa tctgcttaat
    10381 gcgtgttgat ttgggctcag tagtactggg cgttaactta ttcgctttct gaatggtttg
    10441 ctgaataaac gcctcaagct ccctgcgttt agttaactca tcctgcttcg cattggcttt
    10501 attgtgtttt tcttgtgctt gtatatccca aacctcccaa aatgagagac ctttaaactg
    10561 ccgactacgt tccgtcagat tacagcgcca aaatacacgg ctgccaactt gcggaaacaa
    10621 ataaatcgta tcaaccagca atgggtcata agccgcttct aaatgttgag gtctagctat
    10681 atcagtgctc cgctgcaacc aaccctcacg cagaatctct gaccccgagt aatacaaacc
    10741 ccacaaatta acgccaaatg aagaaataga gacctttcgg cgaggcagta acgctactcg
    10801 caactgctct tgctccacag cccttaaact acctgtacga tgctgcatac cccattgcca
    10861 tagctggaca ggaatagacg gtaaatctgt aggaaaatca gcatctcgat cgtatttatc
    10921 catcaccaga tggttatttc tgaataagat cgtacgcaaa ataatttgtg tgaactcaaa
    10981 taccgacaga gatgcatcta acctatagtc tgtttcacca tggcttttga tccgactgcc
    11041 ctctacaatg ccaggtgcaa aggacttaaa ctcggcttgt agtgttctaa aagtgctttc
    11101 cactatgcct ttagcatcgc cacgtctagg tggagcactt tccactcgca cattaaaact
    11161 agaaactaag gcttcgacct gatgactcat taattcgcca cggtccgcta gcaacacatc
    11221 tggcaaacct acacacggcc agtctgagct actaatctca atatcatgct gggcacaaat
    11281 ggccgttttg tcagagcaag catttacaaa agcctgcatc gccaccacat aagacggatt
    11341 ttcaaagccg atataaaagc ccgtgatcat ccgactaaac acatcaatca caatgtaaag
    11401 cgttggtctt cctatgattt tttggcgatc atgatgatcc actaaataaa tatcagcaat
    11461 cgtggcatca atctcataac gactcccagg gcctaacgcc tgagaagtgg ctgtactact
    11521 taagggtcgt acgtcttttt tatatacccc tgctttaact ctagacttta agcgctgagc
    11581 tttagggtat tctcgatcat aaaaataacg aaactgacgt agtgttgggt aatcctcttg
    11641 gggaatgcga ggaaaatact gagcaaacaa gtccacaaat cgtctatagg caacggtggt
    11701 ctttgtacct ttttgattta acaggtgctt ttctatggtc aacctaaaaa ggcgttcaat
    11761 ctcgggcgtt accttggttc cttcaccctt accatattct ctggctcggc caatctttgc
    11821 tgttcctgtc gctgaacgtc tttcccctgg tgcaccgctg tttttgtagt caggaattaa
    11881 tgcattaggc gtttgaccac gctgccagta acggcgtaac aacttataaa ctgtagcctt
    11941 agtaacctta tgttcttgga ccacatgctc aacgagttcg cttctgactt tagggtcgaa
    12001 acgatcctta ctattaataa taggaagaat ttttcgataa tcctcctcgc gcttctggaa
    12061 gctgacagaa tctacagacg gctcttctaa atcaagatgt acataagggt catcagttct
    12121 aactaagcgg ccttcatcta aatactgcat tagcaacaac tcagccctag cttgtggaac
    12181 tcctttatca gcgcttattt gcatccagac aacttggcca tcctctatag ccaaaatgcg
    12241 atacggatca ttatcaaata gcacaacctc attaatttgc cacatagcgc aactcctcca
    12301 tattcactac ttggctaata cagagatctg cacacttatt tgccctgaaa gacttataaa
    12361 tattgaactt aataaaacca tttgcggtta aggctcgtat ctcactcaat gttttgccta
    12421 actccaaatc ataagcaata tcaacctgct tacagacatt gataatgttt tcatctcctt
    12481 tttcttgcag gatatgggcc aatggggata gttgtgctaa aagctccgca gaaacttctt
    12541 ctgttttcac tgaataaagc cattcaatat tttcttttac tacgggattt atttctttat
    12601 cagtaaaaat gaaccaagga atttgctttt gctgccaata gcgacgctct agttctagtt
    12661 tttctaaggt acgctcgtct tgtaaggctg ctgcaggttt gacttgaata gcaaactgct
    12721 caaaaggacc atctttgcag tccactaaaa aatcagtaga cataacctga tctacaccac
    12781 gaataacagg atgcttaata ccactatcta ttgcaatctg cctggtatca ctaggtaata
    12841 aggggaactg ctcgcgtata tctagcacgc tgctctccca ctcaagactg agaaaaacag
    12901 caagctctaa gtcagatagc aaatgatgga ctcgtcccgt cttatgagaa taaatacggt
    12961 gggaacgacc tgaagaagga acttcttgta ctgttagcca tggaatatag tctttaccat
    13021 gcccttggcc acgcccctct ttaatacgac gggcaatttg cacttcagaa aatgaagagt
    13081 ttgctttagc cataacaaaa gtccagtatg ctttttcaca gcataactgg actgatttca
    13141 gtttacaact attctgtcta gtttaagact ttattgtcat agtttagatc tattttgttc
    13201 agtttaagac tttattgtcc gcccacaagc ccttttttat ctatgatagt gattactcaa
    13261 ccgtaaccga ttttgccaag ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga
    13321 catggtaagc cagcagctgc agcggaacgg tgtagaagat cggtgcaatc acctcttcca
    13381 catgcggcat ctcgatgatg tgcatgttat cgctacttac aaaacccgca tcctgatcgg
    13441 cgaagacata caactgaccg ccacgcgcgc gaacttcttc aatgttggat ttcagttttt
    13501 ccaacagttc gttgttcggt gcaacgacga taaccggcat atccgcgtca atcagcgcca
    13561 gcgggccgtg tttcagctcg ccagcagcgt aggcttcagc gtgaatgtag gagatctctt
    13621 tcagcttcaa tgcgccttcc agtgcgattg ggtactgatc gccacggccc aggaacagtg
    13681 cgtgatgttt gtcagagaaa tcttctgcca gagcttcaat gcgtttgtcc tgagatagca
    13741 tctgctcaat acggctcggc agcgcctgca gaccatgcac aatgtcatgt tcaatggagg
    13801 catccagacc tttcaggcga gacagcttcg ccaccagcat caacagctca gttaactgag
    13861 tggtgaatgc tttagtggac gccacgccga tttccgtacc cgcgttggtc atcagcgcca
    13921 gatcggattc gcgcaccaga gaagaacccg gaacgttaca gattgccagc gaaccaaggt
    13981 aacccagctc tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg
    14041 acaaggtgat catcaggctg ttacgacgca cggcagattt acgatagcgg aattcagagg
    14101 cgatttcgac gtcgcacgga atacctgcca gcgattcaaa ccagtagcgg gaaaccatac
    14161 cggagttata agaagtacca caggcgagga tctgaatatg ctcaaccttc gacagcagtt
    14221 cgtcggcgtt cggtcccagc tcgcttaaat caacctgacc gtggccgatg cgcccggtaa
    14281 gggtgttttt gatcgcgttc ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa
    14341 tacctttatc gcccgcgtca tattgcagat tggattcgat atcctgacgt tttacttccg
    14401 cgccagtttt atcgaagatg tttaccgaac ggcgagtaat ttccgcaata tcgccctctt
    14461 caaggaagat aaagcgacgg gtcaccggca gcagcgccag ctggtcagaa gcgataaagt
    14521 tttcgcccat acccaggcca atcaccagcg gactaccaga acgtgccgcc agcagggtat
    14581 ccgggtgacg ggagtccatg atcactgtac cgtacgcacc gcgcagctgc gggatagcac
    14641 gcagaacagc ctcacgcaga gtcccgcctt gtttcagctc ccagttcacc agatgggcaa
    14701 tcacttcggt atcggtttca gaaacgaagg tatagccacg cgcttttaac tcttcacgca
    14761 gcggttcatg gttttcgatg atgccgttat gcaccaccat aatgtgttca gaaacatgcg
    14821 gatgcgcgtt agcttctgaa ggttcaccgt gggtcgccca gcgagtgtga gcaataccgg
    14881 tgccgccatg cagaggatgt tcttccgctg cctgagccag catctggact ttaccgaggc
    14941 gacgcaggcg ggtcatatga ccttccgcat caacaacggc cagaccggca gagtcatatc
    15001 cgcggtattc cagacgacgt aaaccttcaa gaaggatttc tgctacatca cgttgcgcga
    15061 tcgcgccaac aattccacac atagtttttg attccgattt atatcgttgt cggtcaacct
    15121 gtatgcccgt tttttcgggc gccccgagcc ttgtagagag tggggttatt tttatagtta
    15181 ctgcttgtgg gagggagatt atgttatctc ctcatcccag atgaccggat tacccggcca
    15241 gaattacttt ttctttaccg gacgacgcca gccttctttc tgagtctgcg gcacacggct
    15301 gatagctaat gcattttcgc cgacattacg cgtcacagtt gtacccgcag caatggtcgc
    15361 gcctttgcct actgttaccg gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc
    15421 gccgataatg gtcttaaatt tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc
    15481 gatgttaacg ttatcgccaa tttccgcatc gcccaggtaa gtcagatgac cagctttcga
    15541 gcctttaccc agacgcgctt ttttcatctc aacaaagtta ccgacgtgtg caccttccag
    15601 caactcagca ccaggacgca gacgggcaaa cgggccaata gtacaggccg ctgccagatt
    15661 cacgtcttcc acaacggtat acggactgat ttcgcaatca tcgccaatca cgctgttttt
    15721 aatcacgcaa ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat
    15781 aacgttagta tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca gatcaaaacg
    15841 cgccggatcg cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt cggactggta
    15901 aacgcgctcc agacgggaga gttgcaggcg gttattcaca ccttctactt cgcttaaacg
    15961 ttgcggatga acggcgacga tttcacgccc ttcctgatac gccagcgcaa taatgtcggt
    16021 gatgtagtat tcgccctgag cattattgtt ggtcagcttc gccagccagc gtttcatatc
    16081 tgcgccgtta gcgatcagaa tgccggtgtt gatctcctga atctgacgct gctcgtcggt
    16141 ggcatctttg tgctcaacaa tgccggtaac tttgccattt tcacgggtga tacgtccata
    16201 accggtcgga tcatccagtt tcaccgtcag cagaccaatg ccaccctgcg gtttagcatc
    16261 acgcaggcgc tggagtgttt cgacagagat cagcggcacg tcgccgtaga acattaaaat
    16321 gtcttcatca tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg tacccagctg
    16381 ctctgcctga agcacccagt taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc
    16441 gccgtgaccg tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac
    16501 atgctgaacc atcgctttcc cggcaagggt atgcagcact ttcggaagat cggaatacat
    16561 gcgcgtgcct ttgcctgcgg caaggatcac tacgctcata gcattattca acatacgcgt
    16621 cctgactgta atttgagaac gaatttaaac cgcttcacct tgaaaaaact acattttttt
    16681 catcgtgaaa tggacagagg ataaattgtt caataacgga ttatccccgc tgacgaaagt
    16741 gccattttcg accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt accttcattt
    16801 aagcgtcagg tggatgtttt tgctcttatt tcgatcaatg aataaacggg aaaacagggg
    16861 tgttttatat cagcaggatc tatgtgaacg ctcttcagga cggatcacgc gtaaaaaaaa
    16921 ccagcctgtt tccagactgg cttttgtgct tttcaagccg gtgttacatc gcttttttgg
    16981 tcaactcgat aacgcgcagc tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt
    17041 aatctacgtc gccgtgagag ctgctaatgt gctcttcagc cttacgtttc gcttccatgg
    17101 ctcgcgcttc gtcgagatcc tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc
    17161 cagactgcac ttcaagaatg ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt
    17221 tcacgatgcg aatcatacca ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga
    17281 tccccagttc accttcgcta cccgttacct ggattttctc gaccagacca gagaacattt
    17341 gttgctctgc gctgacgacg tccaggtggt aagtcattgc catatcaccc tccgattaag
    17401 gcgttaaagt tttttggctt tttccacagc ttcttcgatg gaaccgacca tgtagaacgc
    17461 ctgctccggc aggtgatcgt attcgccttc catgatgcct ttaaagccac ggatggtgtc
    17521 tttcagggag acgtatttac ccggagaacc ggtgaatact tctgccacga agaacggctg
    17581 ggacaggaag cgctggatct tacgagcacg cgctaccacc agtttgtctt cttcagacag
    17641 ttcatccata cccaggatgg cgatgatgtc tttcagttcc tgataacgtt gcaggatgga
    17701 ctgaacgcca cgcgcggtgt cgtagtgttc ctgaccaacc accagcgggt ccagctgacg
    17761 gctggtggag tccagcgggt caacggccgg gtagataccc agagacgcga tctgacggct
    17821 cagtaccacg gttgcgtcaa ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc
    17881 atccgcaggt acgtataccg cctgtacgga ggtgatagaa ccagttttgg tggaggtgat
    17941 acgttcctgc agaacgccca tctcttccgc cagggtcggc tgataaccta ccgctgaagg
    18001 catacggccc agcagtgcgg atacttccgt accggccagg gtgtaacgat agatgttgtc
    18061 aacgaacagc agaacgtcac gaccttcgtc acggaatttc tcagccatgg tcagaccggt
    18121 cagagcaacg cgcagacggt ttcccggcgg ctcgttcatc tggccataca ccagggatac
    18181 tttgtcgata acgttggagt cggtcatttc gtggtagaag tcgttaccct cacgagtacg
    18241 ttcacctacg cccgcaaaca cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag
    18301 ctccatcatg tttacggttt tacctacacc cgcaccaccg aacagaccaa ctttaccgcc
    18361 cttagcgaac ggacacatca ggtcgataac tttgataccg gtttccagca gttcctgaga
    18421 gtttgacagc tcttcgtagg aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc
    18481 gatctcgcct ttcatgtcga ccggttcacc cagtacgttc atgatacggc ccagagtcgc
    18541 tttacctacc gggacttcga tcgggtgttc gaggtctttt acatccagac cgcgacgcag
    18601 accgtcggag gaacccattg cgatggtacg cacgataccg ccgccgagct gctgctgaac
    18661 ttccagcacc agacgctcat taccattttg cacctcaaga gcatcgtaca cgcgcggtac
    18721 ggcatcctga gggaattcga cgtcaactac ggcgccgatt acctggacaa tctttccagt
    18781 agccatctta aatcctctac gaaataacct gtttaaaccg cggcggcccc cgagacgatc
    18841 tcggtgagtt cctgagtaat gctggcctga cgagctttgt tgtataccaa ctgcagctct
    18901 ttaatcaggc tgccgccatt gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc
    18961 tcgctggcca ggttttcaac cacgccctga taaacctgag attcgacata acgacgcagc
    19021 agggtatcca gcaacgcctt cggatcgggt tcgtacaggt aatcccagga tttatgtttc
    19081 agatcatcat catctgatgc cggtaacggc agcagctggc tgatggtcgg aacctgagac
    19141 atggtgttaa taaatttgtt gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag
    19201 gcctgcaaca tcacttttac cggaccgatc agttcggaca gggaagggtt atcccccatg
    19261 ccggtgacct gggcaacaac attgccgccc acggagttga agaacgacac gcctttcgag
    19321 ccgatcattg cgaggtcgca ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc
    19381 agttttttga acaggttaat gttcaaacca ccgcacaaac cacggtcggt cgacaccacc
    19441 aggtagccca cgcgtttaac gtcgcggtct tccaggtaag ggtgcttata ttccagatta
    19501 ccatgtgcaa ggtgaccaat cactttgcgc atggtttctg cataaggacg gctggccgcc
    19561 atgcgatcct gcgatttacg cattttggaa gcggcgacca tctccatcgc tttagtgatc
    19621 ttttgcgtgt tctggacgct tgcgatctta ctacgtatct cttttgcgcc ggccatgagc
    19681 ttctcctcaa tgccttgcgg cctgccctaa ggcaagccgc cagacgttac caggattggg
    19741 ttgctttgaa ggaatcgagg atgcctttca gtttgccttc gatttcgtcg ttgtagccac
    19801 cggtctggtt gatctcttgc atcaacggag cgtgatcacg gtcgacgtaa gccagcagag
    19861 cggcttcgaa gctgccaatt ttcgacagtt caacatccgc caggtaacca cgttctgctg
    19921 cgaacagaac cagagactgc tgcgcaacgg acatcggcgc atactgtttc tgtttcagca
    19981 gttcggtcac tttctgaccg tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg
    20041 caaactgaga gaacgctgct agttcacgat actgtgccag agcggtacgg ataccaccgg
    20101 acagtttttt catgatcttg gtctgtgctg caccaccaac acgggatacg gaaatacccg
    20161 ggttaaccgc aggacgaata ccggcgttga acaggttggt ttccaggaag atctgaccat
    20221 cggtaatgga gattacgttg gtcggaacga acgcagaaac gtcacccgcc tgagtttcga
    20281 taatcggcag tgcggtcaga gaaccggttt tccctttcac ttcacctttg gtgaaggctt
    20341 caacgtattc ggcgttaaca cgtgcagcac gctccagcag acgagagtgg aggtagaaaa
    20401 cgtcgcccgg gaatgcttca cgtcctggcg gacgacggag cagcagggag atctgacggt
    20461 aagcaacagc ctgtttagac aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac
    20521 ggaagtattc gcccattgcg caaccggcat acggtgccag gtattgcagt gcagcggatt
    20581 cagacgcggt tgctaccaca acgatggtgt tagccagtgc gccgtgctct tccagtttac
    20641 gtaccacgtt agaaatggtg gacgctttct ggccgatagc gacatagata catttgatac
    20701 cggaatcacg ctggttgatg atggcatcga tagccagtgc ggttttacct gtctgacggt
    20761 caccgatgat caattcacgc tgaccacgac cgattgggat catggagtca acggctttat
    20821 aaccggtctg taccggctga tctacggact gacgttcgat aacgcccgga gcgattgctt
    20881 ctacagcaga gaagccgtcg tgatccagcg gacctttacc gtcgattggt gcacccagag
    20941 tgttaaccac acggcccagc aggccacggc caaccggaac ttccaggata cggccagtac
    21001 acttaacttt catgccttcg gcaaggtcag cgtacggacc cataacaacc gcacctacag
    21061 agtcgcgctc gaggttcagt gcgatagcgt aacggttacc cggcagggag atcatttcac
    21121 cctgcataca atcggccagg ccgtgaatgc ggataacacc gtcacttaca gaaacaatag
    21181 taccttcgtt gtgagcttca ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt
    21241 cgctgatttc ggtggaattc agttgcatgc tccagtcccc ttaagactgc aagacgtctg
    21301 caaggcgctc aagacgaccg cgtacgctgc catcaatgac catatcaccc gctcggatga
    21361 taacgcctgc cattacagac ttatcgattt tgcaattcag cttaactttg cgtgacagac
    21421 gtttttccat cgcagcagaa attttcgcga gctgttgttc actcagtgcg gcagcggaaa
    21481 tgacgtctac ctcagcggta gcctcactca cggcacgaag gtgaataaac tgctccagaa
    21541 catccgggag cgcgttaaga cgaccatttt cagccattac ccgaatcagg ttctgaccgt
    21601 tttcgtccag ttgctcacca caaactgcga taaacgactc ggcgagcgtt tctggcgcaa
    21661 gcgcgccaga gagaagctct gccatttgtt cgtttttggt tacctcggcg gcaaacgcca
    21721 gcatgtcctg ccagcgttct acactttggt gttcgacggc aaagtcaaaa gctgctttgg
    21781 cgtaggggcg agctaccgta ataaattcag acatcagccc ctccctcctt acagttcagc
    21841 gacaagttta tccacgatgt cgctgttagc agcttcatcc acggaacgtt cgatgatctt
    21901 ctcggcgcca gcaacagcca ggatagcaac ttgcttacgc agctcttcac gggcacgttt
    21961 acgctcggct tcaatttctg cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc
    22021 ctcagctttc gcttcgtcca gaatctgcga gcggcgtttg ttcgcctgct cgatgattac
    22081 ctgggcttcc gctttcgctt ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag
    22141 gtccttatat gctcgttctg cggaagcaag gccgtcagca atttcttttt gacgtttttc
    22201 gatggctgcc attaatggcg gccatacgta cttcatgcag aacagaacga acaggacaaa
    22261 cgcgatggcc tggccgagga ttgttgcgtt aagattcaca gcacaatgcc tctatttagt
    22321 taacgttctg atattgctct ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca
    22381 cgtacagacc cagacctaca gcgatcatcg ggatagcatc caccagaccc ataacgataa
    22441 agaactgagt acgcagcaga ggaatcagat caggttgacg cgctgcgcct tccaagaatt
    22501 tacccccgag gatgccgata ccgatcgcag caccgattgc cgccagaccc atcatcacag
    22561 cggcagccat gtacagcaga tccatattca ggttttccat gacagtctcc agtttgtttc
    22621 agttaaaacg tagtagtgtt ggtaaattaa tgttcttcag acgccatcga cagatagacg
    22681 atcgtcagaa ccatgaagat gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc
    22741 cacggcacat tcaggatcca ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa
    22801 atcagctcac cggcatacat gttaccgaac agtcgcaaac cgagtgaaac tggtttggac
    22861 agcaggctta ccccttcaag gattaagttg acaggaatga acgcccagtg attgaacggc
    22921 tgcagcgtca actctttcgt gaagccgccg atgcctttca ttttgatgct gtagaacaga
    22981 atcaggataa atacgcccaa tgccatagac agcgttacgt tcacgtccgc agacggaacc
    23041 acacgcagtg caggcagacc cagtacatgt tcagcaatgt acggcagcag gtcgataggc
    23101 agtaaatcca tcaggttcat caggaatacc cagacgaaga tcgtcagggc cagcggagca
    23161 atcagcttgc ttttgccatg gtacatgtct ttcacgctac cattaacaaa gccgatcacc
    23221 agctcaatcg cggtctgaaa cttacctggc acaccgctgg tcgccttttt ggctacgcta
    23281 cggaataaaa ccaggaacaa cagacccagc accaccgaga agaacatgga gtcaatattg
    23341 attgtccaga aggtggctgg ggggttttgt ggatccacca gcgagaatgt acgcaggtcc
    23401 agctgaaggt tattcaggtg gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc
    23461 atgatgcctt ttaccctttg ttgttaatta cagccggtgc cagtatctga accaccagca
    23521 ccaaaaccca cgtaacgatc agcggcaaga ataccgcctt taaaaccgcc aacgccacca
    23581 ccagtaacac caacatcgcc agaactttga aagcttcgcc aaatgcgaat gtccaggcca
    23641 cccggccttt cgctggtgta tgcgcctggt gacgccaggc aaatatcata aacaaaacgt
    23701 taggcagaaa gactgccagg cccccgctta ttgcagagac gccccagaag gggtctttga
    23761 ggctgaacag caatccactt gctatcacca ccagtaactg aacgagcaga agcttccgag
    23821 caacgtttcg actcacgagc gacacagaca tcacgttttt cactcctgct cccttcgagg
    23881 tatgccgcgt gtcgtataaa actttcttta aggcttagag tcaagcatca aaaagaggtc
    23941 aaattatacg gcgcgccccc gtgatttcaa acaataagta gccaaaaggt gaataaatgt
    24001 ttaaatattt ttccagtgca tacaattgcg acttttctgc taaccctgtt cgatcatgaa
    24061 aaactgtaaa taacgcgtaa acactggtga taaagcgtgc ttcagatcac atattgcgca
    24121 tgttcgcgca cagcatattt atttacttgg caaatgatgc ctttgcaagt ttatgatatt
    24181 tcactctaaa aacagatact gttttaataa atgacattta cacaacaaaa accacccatt
    24241 gacattttta ataatgtttt agcagccaat gatggttctt agcgccgatt tttagcagac
    24301 tgatattttc actaatgact tattttctgc ttaccaaaaa aagccacgtt atcttgttga
    24361 tgcaaaagag tgaacgtggc gttaaatgta accagttata tcagtagaaa acctggttgt
    24421 tgttaacagt ataaccggtc aattttttat gatttttttg ataaaaatta aattttattt
    24481 gctttaatca ccaccagatg acgttcgcca tccagggctg gaacctgaag tttaaccact
    24541 gattcgacct gatattcttc gggcaacaaa gcgatttcat cttccggcct ttgccctttc
    24601 agcgcgtaga aacggccttg ctcaccagga agatggtggc accagctcac catatcgttc
    24661 agagaggcaa aagcgcggct aattacgcca tcaaatggcg gctctgaagg aaactcttct
    24721 accctgctct gtactggttc aatattctcc agtttaagct catgttgcac ctgacgaagg
    24781 aaacgcacgc gtttaccaag gctatccaac agagtgaaat gggcttcagg acgcacgata
    24841 gagagtggaa tgcctggcag tcctggtccg gtgccgacat cgataaaccg ttcaccttgc
    24901 agatacggtg ccaccacaat gctatcgaga atatggcgta ccagcatctc attaggatcg
    24961 cggaccgaag tcaggttgta cgctttgttc catttatgca gcatattcac gtaggcaata
    25021 agctggtttt tctggtgatc ggtaagcgaa atacctgcgt ctttcagcag taaggagagt
    25081 ttgttgagca cggtgattac ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag
    25141 acatttttaa tgcgttatgc gctacgacgc agcataccct gttttttcag ccacaccagc
    25201 agaatggaga tggccgcagg cgtgacgcca gaaatacgcg aagcttggcc gatagaggct
    25261 ggtttgtgat cgttaagttt ggcgatcact tcgttagaaa gaccggatac ctggcggtaa
    25321 tccagtgtcg cgggtagcag ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc
    25381 tggcgcgcga tataaccttc gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg
    25441 tctgtcaacg caggggcaaa cggcgtcagc gtggttaatt tttcataagt catttccgga
    25501 cgacgcagca gatcttcacc actggcttca cgggaaagcg gcgcagtcag gtgagcattc
    25561 acttcggctg cagcttccgc cgacggggtt acccaggtcg atttcagacg ctgacgctca
    25621 cgctcgatat tctcaagttt ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc
    25681 agttcacgac cgatttcagt caaacgcaga tccgcattat cttcgcgtag catcagacga
    25741 tattctgcgc gcgaagtaaa catacgatac ggttctttgg ttcctaaagt gcacaggtca
    25801 tcaactagta cgccgagata cgcctgagaa cgtgccggag cccaaccttc tttgtcagca
    25861 gacagacggg cagcgttaag accggccagc aaaccttgcg cagcggcttc ttcgtaaccg
    25921 gtagtgccgt taatctgacc agcaaagaac agcccctgga taaacttgct ctccagcgtc
    25981 ggtttcaggt cgcgaggatc gaagaagtca tactcaatgg cataacccgg acgcacgatc
    26041 ttcgcgtttt ccatcccctg catagagcgg acgatttgca tctgcacatc gaacggcagg
    26101 ctggtggaga taccgttcgg ataaatttca ttagaggtca gtccttccgg ttcaaggaag
    26161 atctgatgct gatttctgtc ggcgaagcgc atgactttgt cttcgatcga cgggcagtag
    26221 cgtgggccga caccttcgat cacccctgca tacattgggc tacgatcgag gttactgcgg
    26281 atcacatcat gggttttctc gttggtatga gtgatataac acggcacctg ctggggatgc
    26341 tgggacgcat tgcccataaa cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc
    26401 agcacactaa agtcgatagt tcgagcatca atacgcggtg gtgtcccggt tttcagacga
    26461 ccaacgcgca gcggcagttc acgcaaacgg tgagaaagcg gaatggacgg cggatcacca
    26521 gcacggccac cgctgtaatt atccagaccg atatgaattt taccgtcgag gaacgtccca
    26581 acggtgagca cgacggcttt ggcacggaac ttcagtccca tttgggtaac agcaccgacc
    26641 acgcgatcgt tttcgacaat aagatcttca accgcctgct ggaagatcat caggttcggt
    26701 tggttctcca gcgccgtacg taccgcctga cggtagagca cacgatccgc ctgagctcgg
    26761 gtagcgcgaa ccgccggtcc tttgcttgcg tttagtatcc taaactggat acccgcttga
    26821 tcgatcgctt tcgccatcag accgtcgagt gcatccactt cttttaccag atgtcccttc
    26881 ccaataccgc cgatcgccgg gttgcagctc atctgtccca gagtgtcgat attgtgtgtc
    26941 aaaagcagag tctgttgacc catacgcgcc gcggccatcg cggcctcggt gcctgcatga
    27001 cccccgccaa tgatgatgac gtcaaaagga tccggataaa acatggtgat tgcctcgcat
    27061 aacgcggtat gaaaatggat tgaagcccgg gccgtggatt ctactcaact ttgtcggctt
    27121 gagaaagacc tgggatcctg ggtattaaaa agaagatcta tttatttaga gatctgttct
    27181 attgtgatct cttattagga tcgcactgcc ctgtggataa caaggatccg gcttttaaga
    27241 tcaacaacct tgaaaggatc attaactgtg aatgatcggt gatcctggac cgtataagct
    27301 gggatcagaa tgaggggtta tacacaactc aaaaactgaa caaccgttgt tccttggata
    27361 actaccggtt gatccaagct tcctgacaga gttatccaca gtagatcgca cgatctgtat
    27421 acttatttga gtaaattaac ccacgatccc agccattctt ctgccggatc ttccggaatg
    27481 tcgtgatcaa gaatgttgat cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg
    27541 agttctgcct cgagtttatc gatagcccca caaaaggtgt catattcacg actaccaata
    27601 ccgattgcgc caaagcggac cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag
    27661 aaaggagaaa ggttgtccgg aatatctccg gcaccgtggg tggagctgat aatcagccag
    27721 atccccgagg cagataaatc ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc
    27781 gcctcttcca gcttttcagc caggtgttct gctacatatt cggcaccgcc gagggtgctg
    27841 ccgctgataa gagtgatatc tgccataaac cgccaccttt attaagagtg gcgtattgta
    27901 cgctgtgaac gcgttgggat ctacctgtgg aaaagtatgg gattaaaaaa gccgatcagg
    27961 gcttgatagt acgcatgatc gggttttgca ggacgatcaa tgtctcggtg gactgaattt
    28021 catcaattgt ttggatcttg ttgataagta catgctggag agcgtcgatc gaacggcaca
    28081 ttacttttat aaagatgctg tagtggcctg ttgtgtaata ggcttcagtg acttcatcaa
    28141 ggctttccag ctttgccagc gcggaagggt agtctttggc gctctttaat ataatgccga
    28201 taaagcagcc tacgtcataa ccgagctgct tcgggctgac atcaatacgc gccccggtaa
    28261 tgatccccgc ctgcttcatt ttctctaccc gaacgtgaat cgtccccgga ctgacgccaa
    28321 attgtttcgc cagttcggcg taagcggtgc gcgcattgcc cattaatgct tccaggatgc
    28381 cacggtccag attgtcgatc agataatttt ccataggatt ttcttatgca gattgatgat
    28441 tcattctatt ttagccttct tttttaatga atcaaaagtg tgttaggctt tttattgaat
    28501 gattattgca tatgtgtcgg tttttattgc ttaatcataa gcaacaggac gcaggagtat
    28561 aaaaaatgaa aaccgcttac attgccaaac aacgccaaat tagcttcgtg aaatctcact
    28621 tttctcgtca actggaagaa cgtctggggc tgatcgaagt ccaggcaccg attcttagcc
    28681 gtgtggggga tggcacgcag gataacttgt cgggctgtga aaaagcggtg caggtaaaag
    28741 tgaaagctct gcctgatgcc cagttcgaag tggttcattc actggcgaag tggaaacgtc
    28801 agaccttagg gcaacacgac ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc
    28861 ttcgccccga tgaagaccgt ctttctccgt tgcactcggt ctatgtagac cagtgggact
    28921 gggaacgcgt aatgggcgac ggtgagcgtc aattctcgac tctgaaaagc acggtagagg
    28981 cgatctgggc gggaattaaa gcaaccgaag ctgcggttag cgaagagttt ggcctggcac
    29041 cgttcctgcc ggatcagatc cacttcgtac acagccagga gttactgtct cgttatccgg
    29101 atctcgatgc caaagggcgt gagcgggcga tagcgaaaga tcttggcgcg gtattccttg
    29161 tcgggattgg cggcaagctg agcgatggtc atcgccacga cgtgcgcgca ccggattatg
    29221 atgactggag caccccgtca gagctgggtc atgcgggtct gaacggcgat attctggtgt
    29281 ggaaccctgt actggaagat gcgtttgagc tttcttccat ggggatccgc gtggatgccg
    29341 acacgctgaa gcatcagctg gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc
    29401 atcaggcgct gctgcgcggt gaaatgccgc agaccatcgg cggcggtatc ggccagtctc
    29461 gtttgactat gctgctgctg caactgccgc atatcggcca ggttcagtgt ggagtatggc
    29521 cagctgctgt tcgcgagagc gtcccttctc tgctgtaata atttatcgcc gccagcgtct
    29581 gagcaggcgg cttcgcatcc cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat
    29641 gccgggtttg ccgtgtgccg acatcgccac ggcatgaaag cgatgctgat gtacccgctg
    29701 cagctctttc actttactcg tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat
    29761 caccaccgca tcggcatcaa accattccct gctttgcaag cgttccataa tggcgcgaaa
    29821 acaactggca agatcggtgc cgccacgaaa cagctggctt aaaaaacgga ttgcttgttc
    29881 gatgccttgt gggcctgaaa gctcataacg gacgatctcg gtggaaaata gcataatata
    29941 gcagcgccgg ttttctgcga gagcaatgcg catcaaggcc aggcagaacg ctttcgcaca
    30001 ctgttcatta aagccgccca ttgagccgga agtatccaca cagacaataa acggcccgcg
    30061 cggctgttca tcgtaatctt tatgtactac cggacgttcg atcacttttt cacgccacga
    30121 ctcaccgtgc aggcgatagg tgagcaactg tttttccacc agccgacggt aaaactcata
    30181 ctccagttcc gttatcccta gtgtcgccag ttctggcggc aggagacgta aaatatcatc
    30241 gctttgttgc agaccatcaa cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg
    30301 gaaggtttcc atctgcgcat cgttgcgcgg tattgatttg gcttcccgag aacgccccag
    30361 ctgctctgcc aggcgtttca gttccggctt ttcgttaaga aattcaccgt atttcacaat
    30421 caactgatag tcgccacgtt taagctggcc ggcgctcata tcccacagac gaccagctgc
    30481 ggtattgtta tctgcgagaa tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac
    30541 ttcacttaac agttgttcgc gttcttcttc taatagctgt tgatttaacg tcgttgcttg
    30601 cacgatcaga cttaaacgcc agcgctggag aaaaagcgtg tgtaacgctg aagttattgt
    30661 gctgttagca tcaaccaact gccgggcttg ttctgcccat ggggaattca gacgatgcag
    30721 taagtccagg atctgtggta gctgcacaat aaactgcggc gtggagagga gctggctttg
    30781 ctgatagcac atcacctctt cggtgagttc tggcgggact cgggcatctt tcagccgact
    30841 gcgcagcgcc tcacgccagc ggggaacatc atcagtgatt gctgccttca gtcgtgggaa
    30901 tttttcaaag aagaccgcca gctgcggtga ggccagcagt gcgatgatca tctcttcgat
    30961 caatccctct tcgctgacgg ccagcatcac attaagcgta tccagcgtta gcattgttgt
    31021 gcctggcgaa tctgtgcacc gacatcctgt aggctggctt caatgcgacc taaccagtcg
    31081 ccagggataa acagacattt ttgctgttcg ctgaataaag cgtgttgctt acgccagtcg
    31141 ctttcaagct cttccagttg ttgttttatt tcacctggca gaccttcagc cgatgaaccg
    31201 ggaagtgcca gcgtactgcc ttgtaaactt acatcgcgta caacaagatg ttgggtgcta
    31261 tcaacttcca gattcagttt ctgggcaaag ccgatacagt tcagtttgcc gcgaatttca
    31321 ccacctttac tcagccactg ttccagcgcg ctacgctcaa aggagatatg aaccacttcc
    31381 atatcatgca gttttaacgg tttttgcagc agcagagtca gagtggaagc agtaacgtta
    31441 acagggagtt gatactgctg acgacggcta aaaatgccgc ccagacgaat taccgttaag
    31501 gctgttttat cgctttgttg ctgctgaagt tgcaggtgac gttgcacaat cgcgcccagg
    31561 cgggtcaaca tcccttgctg ttgccaggcg tgaccggtca tcaatacatc aatttgttgt
    31621 tgtatcaaat tcaggctttg cgcgtcatac cacaggcaat ctttcagcaa aatgagatct
    31681 accggggcaa cagcactgcg accgctaaaa aaggcgctgg cctgcaataa tcggatcgct
    31741 tttttccagc gacgatccga gacataaggc gcatccggta atttatccag ttgctggcgc
    31801 agcataaaaa tcagctcaaa tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc
    31861 cagcgttcat attcttcatc tgtgacctgc aaggcatcag gaaccggatt gtcgttttca
    31921 tcctgttgac tggtcagcat ggagcggaaa ttcgctttat cctgcacttt atctaaccac
    31981 agacgaatca gcatgcggtc atataacgct tccagactgc tgtctgcttc cggcagctcg
    32041 ttggaggccg ccaccagcag acgcatcggg attttttcta cgtgtgcacc gttgcggaac
    32101 tggcgctcgt taatggcggt gagcaaggta ttaagaattg ccgggcccgc tttccagatc
    32161 tcatccagaa agacaatttc tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc
    32221 ccttcatctt ttagcgcctg aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag
    32281 cgggtcatca gatattcaaa cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg
    32341 atcaaacttt tggcaatacc tggcgggcca aggaggaaca cactttcacc acttaatgcc
    32401 gctaataaac acaagcggat ggcgtggcta cgttcataaa gccccttttc caacgaactg
    32461 ctcaggcggg aaattctttc cgctaataaa tgagggtgag ccataatgaa gtggcgtcct
    32521 ttcgtcaaaa gttctgcgta aattgcgagt atagacgttt cttgctggtg gctaaaatag
    32581 tctcaaaggg ggg
//
LOCUS       DAJTNA010000024        32288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTNA010000024 DAJTNA010000000
VERSION     DAJTNA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32288
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.024"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            197..991
                     /gene="panB"
                     /locus_tag="OO161_000978"
     CDS             197..991
                     /gene="panB"
                     /locus_tag="OO161_000978"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5462136.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1003..1854
                     /gene="panC"
                     /locus_tag="OO161_000979"
     CDS             1003..1854
                     /gene="panC"
                     /locus_tag="OO161_000979"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5462137.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="OO161_000980"
     CDS             complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="OO161_000980"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5462138.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3316..3696
                     /gene="panD"
                     /locus_tag="OO161_000981"
     CDS             3316..3696
                     /gene="panD"
                     /locus_tag="OO161_000981"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5462139.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3700..4899)
                     /locus_tag="OO161_000982"
     CDS             complement(3700..4899)
                     /locus_tag="OO161_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013991358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5462140.1"
                     /translation="MLFTASVSAALPARYMQTIENAAIWAQIGDKMVTVGNIRAGQII
                     AVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSNQNLITWKDTP
                     VYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYISALDAQPDNGL
                     PVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQLEGYVKNKIN
                     LPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKWDPKSLQFMSV
                     SELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRALAQFNPHVWY
                     LSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILRTDSLETMSRL
                     VSNQPQG"
     gene            complement(4994..5434)
                     /locus_tag="OO161_000983"
     CDS             complement(4994..5434)
                     /locus_tag="OO161_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5462141.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLLLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5538..6308)
                     /locus_tag="OO161_000984"
     CDS             complement(5538..6308)
                     /locus_tag="OO161_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5462142.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(6305..7231)
                     /locus_tag="OO161_000985"
     CDS             complement(6305..7231)
                     /locus_tag="OO161_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5462143.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7340..8002
                     /gene="can"
                     /locus_tag="OO161_000986"
     CDS             7340..8002
                     /gene="can"
                     /locus_tag="OO161_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5462144.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVIQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8043..8579)
                     /gene="hpt"
                     /locus_tag="OO161_000987"
     CDS             complement(8043..8579)
                     /gene="hpt"
                     /locus_tag="OO161_000987"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5462145.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFVADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8785..11175
                     /gene="gcd"
                     /locus_tag="OO161_000988"
                     /pseudo
     CDS             8785..11175
                     /gene="gcd"
                     /locus_tag="OO161_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
     gene            complement(11222..12772)
                     /gene="cueO"
                     /locus_tag="OO161_000989"
     CDS             complement(11222..12772)
                     /gene="cueO"
                     /locus_tag="OO161_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5462146.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12938..13285
                     /locus_tag="OO161_000990"
     CDS             12938..13285
                     /locus_tag="OO161_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5462147.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13391..14257
                     /gene="speE"
                     /locus_tag="OO161_000991"
     CDS             13391..14257
                     /gene="speE"
                     /locus_tag="OO161_000991"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5462148.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14273..15067
                     /gene="speD"
                     /locus_tag="OO161_000992"
     CDS             14273..15067
                     /gene="speD"
                     /locus_tag="OO161_000992"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5462149.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15104..15466)
                     /locus_tag="OO161_000993"
     CDS             complement(15104..15466)
                     /locus_tag="OO161_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5462150.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15641..18238)
                     /gene="acnB"
                     /locus_tag="OO161_000994"
     CDS             complement(15641..18238)
                     /gene="acnB"
                     /locus_tag="OO161_000994"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5462151.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18593..20243
                     /locus_tag="OO161_000995"
                     /pseudo
     CDS             18593..20243
                     /locus_tag="OO161_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20314..21738)
                     /gene="lpdA"
                     /locus_tag="OO161_000996"
     CDS             complement(20314..21738)
                     /gene="lpdA"
                     /locus_tag="OO161_000996"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5462152.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22063..23955)
                     /gene="aceF"
                     /locus_tag="OO161_000997"
     CDS             complement(22063..23955)
                     /gene="aceF"
                     /locus_tag="OO161_000997"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5462153.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPLSLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(23970..26633)
                     /gene="aceE"
                     /locus_tag="OO161_000998"
     CDS             complement(23970..26633)
                     /gene="aceE"
                     /locus_tag="OO161_000998"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5462154.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDDLLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26794..27558)
                     /gene="pdhR"
                     /locus_tag="OO161_000999"
     CDS             complement(26794..27558)
                     /gene="pdhR"
                     /locus_tag="OO161_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5462155.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERICELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28099..29472
                     /gene="aroP"
                     /locus_tag="OO161_001000"
     CDS             28099..29472
                     /gene="aroP"
                     /locus_tag="OO161_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5462156.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29515..30369)
                     /gene="ampE"
                     /locus_tag="OO161_001001"
     CDS             complement(29515..30369)
                     /gene="ampE"
                     /locus_tag="OO161_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5462157.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30366..30917)
                     /gene="ampD"
                     /locus_tag="OO161_001002"
     CDS             complement(30366..30917)
                     /gene="ampD"
                     /locus_tag="OO161_001002"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5462158.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31005..31898
                     /gene="nadC"
                     /locus_tag="OO161_001003"
     CDS             31005..31898
                     /gene="nadC"
                     /locus_tag="OO161_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5462159.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32101..>32288
                     /locus_tag="OO161_001004"
     CDS             32101..>32288
                     /locus_tag="OO161_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5462160.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGG"
BASE COUNT         7786 a         8770 c         7939 g         7793 t
ORIGIN      
        1 gccacgctac aacaggatgg aactatacca atagaagctg gtgagtttaa agccaccagt
       61 actttccaag taacctaccc ttaataagtc caccgcaccg ccatcatctg gcggtgcggt
      121 aattgataaa tcattgcccc caccaatgtg actgataaaa tgccaggcaa cacgacatca
      181 tttatcagga cacgttatga aaccgaccac cattgcttca cttcagaaat gtaagcagga
      241 taaaaagcgt ttcgcgacca tcaccgctta cgactacagc ttcgcgaaac tatttgctga
      301 tgaaggactt aacgtcatgc tggtaggcga ctcgctgggc atgacggttc agggtcacga
      361 ctccaccctg ccggtgaccg ttgccgatat cgcctaccac actgcctccg tacgtcgtgg
      421 tgcgccaaac tgcctgctgt tggctgacct gccgtttatg gcgtatgcca cgccggaaca
      481 agcctttgaa aacgccgcaa cggttatgcg tgccggtgcc aatatggtca aaattgaggg
      541 cggtgagtgg ctggtcgaaa ccgtaaaaat gctgaccgaa cgtgccgttc ctgtatgtgg
      601 tcacttaggt ttaaccccac agtcagtgaa tattttcggt ggctacaaag ttcaggggcg
      661 cggcgatgaa gcgggcgatc aactactcag cgatgcatta gccttagaag ccgctggggc
      721 acagctgctg gtgctggaat gcgtgccggt tgaactggca aaacgtatta ccgaagcact
      781 ggcgatcccg gttattggca ttggcgcagg caacgtcact gacgggcaga tcctcgtgat
      841 gcacgacgcc ttcggcatta ccggcggtca cattcctaaa ttcgctaaaa atttcctcgc
      901 cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt
      961 ttatccgggc gaagaacaca gtttccatta aggagtcacg ttgtgttaat tatcgaaacc
     1021 ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg
     1081 gtgcctacta tgggtaatct gcacgatggc catatgaagc tggtcgacga agccaaagcc
     1141 cgcgccgatg tggtcgtcgt cagtattttc gttaacccga tgcagttcga ccgcccggaa
     1201 gatctggcac gttatccacg gaccttgcag gaggactgcg agaagctaaa caaacgtaaa
     1261 gtggatttag ttttcgcccc ttcggtaaaa gagatctacc cgaacggtac tgaaacccat
     1321 acttacgttg acgttcccgg gctttcgacc atgctggaag gcgcgagccg tccggggcat
     1381 tttcgcggcg tttcgactat cgtcagcaag ctgtttaacc tggtccagcc ggacatcgcc
     1441 tgcttcggtg aaaaggactt tcagcaactg gcgctaatcc gcaaaatggt tgccgatatg
     1501 ggcttcgata ttgaaattgt cggcgtcccg attatgcgcg ccaaagatgg ccttgcgctc
     1561 agttcccgta atggttatct gacggcggaa caacgcaaaa ttgcgcccgg tctgtacaaa
     1621 gttttaagtt caattgccga caaattgcag gctggcgagc gggatcttga tgaaattatt
     1681 gccattgcag ggcaagaact gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc
     1741 gatgccgaca cgttgctgga agtctctgaa accagcaaac gagctgtaat tctggtagcc
     1801 gcctggcttg gcgatgcccg cctgatcgac aacaaaatcg tcgaactggc gtaatgcttt
     1861 tactctaaat aattcaagtt gcaggaaggc ggcaagcgag tgacaaattc gtcaggaacg
     1921 aatttgccca gccgcaggct ggccttcggt gagagacatg gatgtctctc attaatcccc
     1981 aggagcttac ataagtaagt gactggggtt agcgaacgca gacacagcac atgcaattta
     2041 aagtatgacg agtataactg gcgctacggc tgatggcgcc aattattaat ttgccccacg
     2101 tcatggcgat ggcgtctgta agcaattatt cataacgcag acaacttagt taatcagcct
     2161 atcaatctca gcaagagata aatttgtcat ctctgcaacg tcttcccgag acatcccttt
     2221 actcagaaaa cgctgggcga attcttgcct ttcctcctgt cttccctgct gaatcccctt
     2281 ctcaatccct ttctcttcaa accactgcgc cagcgtcatc atagactccc ctcccgtttc
     2341 cctgtctctc agcacaccat aaaacaaatc cgcttgttca gtatgaccgc gttgcagcat
     2401 atagttttgc atggcaacta actgacttcc gctagtgtac ccttcgtcaa tcagcgtgac
     2461 cagttgctcc agcaataaca ttaagtcgcg ctggcgaata tgtttttgca gtagttcgag
     2521 aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt gtgatggtga tatccaccag
     2581 cgggaaagga ctgttataga cgcgtcgtgc cagctccggc gagtaaaata tatcaaacca
     2641 gcacattgag agcggataag gtgtggcctc gccctgataa aacagtatcg gcaccaccag
     2701 cggcagctta tcgtgatccg cctccagatg ccggtgcatg gcagcaatag aataacgcat
     2761 catgcgaaag gccattttct tatccggctt gctttggtgt tcaatcacaa catgcagata
     2821 accgggattg ccctgcattt gcacggaata gagcacgtcc gtgctgtgtc ctttcaggct
     2881 ctcttcaatg aaactccccg actctaaatg aagcgtgttg aggtcacaaa gttctcgtaa
     2941 ttccactggc aaatgtatct ccagaaagtc gcgagccgtc tccgcgtgca ttaaaaattg
     3001 tttaaacact gcatcatgcg gtgtggtact cggtgcatcc atcttccctg ccttccgtgt
     3061 tgtcttgcgt gaaggcagta tccagtcaag gcgtgccatc gtcagccacc tctttagtgt
     3121 ttaccgagca gcgttcagag aaatttcaat tggttacagc cgttacatgg cacgcttcgg
     3181 cacggtacgc aaactgctga tgtaaatgcc gtcagatgag tagacactaa acaaaaatcg
     3241 ggcaatactg cgtgagaatt tcctcaaagc aggcatcgcc tgcttcgtta acgacagggt
     3301 agaaaggtag aagttatgat tcgcacgatg ctgcagggca aactccaccg cgtgaaagtg
     3361 actcatgcgg acctgcacta tgaaggttct tgcgccattg accaggattt tcttgacgca
     3421 gccggtattc tcgaaaacga agccattgat atctggaatg tcaccaacgg caagcgtttc
     3481 tccacttatg ccatcgcggc agaacgcggt tcgagaatta tttctgttaa cggtgcggcg
     3541 gcccactgcg ccagtgtcgg cgatattgtc atcatcgcca gcttcgttac catgccagat
     3601 gaagaagctc gcacctggcg acccaacgtc gcctattttg aaggcgacaa tgaaatgaaa
     3661 cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt tatccctgcg gctggttact
     3721 caccagccgc gacatcgtct ccagcgaatc cgttcttaag atataaagtc gttttagtaa
     3781 caacggatta tcccccggtt ttactttgcc tttcatggtt gtcaccgcca ggtgaaatcc
     3841 ggcatcgttt gctgccttca cggctttgtc attaaatccg ccaaacggat acgaaagata
     3901 ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat caaacagaat
     3961 attgtgttca ctacggctca gtaatatggg gcggcgataa ccatccaccc gatgcaaaaa
     4021 atgggtatgt gactggaaat caaaaacatc gcgaatttcg ttaagttcag aaacgctcat
     4081 aaattgcagc gattttgggt cccacttctg cgggtgacgt ttgatgcgcg aggtaacaat
     4141 aaacgccgtc gccttcatgc catattgttt caacacggga tacgcatagc ggctcaccga
     4201 tttgaggcca tcatcaaagg taatcaccac cgctcgcgca gggagattga tcttattctt
     4261 cacgtaacct tccagctgca ccatgctcag tgtcgcgtat cccctgtcgc gcagccaggc
     4321 catctggtta ttgaaagcgc gtactgaggt ggtcgtcgaa gtatggcgaa aacgggtgtt
     4381 ttcttcatcg cgcagaatat ggtgataggt tagcaccggc aggccattat cgggttgggc
     4441 atccagcgcg ctgatatagg ccagtcgatc gccaatacgg atctgatacc aggtttgatt
     4501 taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca gtaccccaaa
     4561 tggcgcactg cccgcactcg gcgcgttata gaccggcgta tctttccagg taattaagtt
     4621 ctgattactc agcggcttgt tgaggtcacc caaaccgtct tcaacttttt gtcgcccctg
     4681 aactggctcg agatgccctt tatcgataaa ccctttacca aagccaaaat taaatgcgta
     4741 ataacttgcg gcagtgggct ccacggcgat gatttgcccg gcccgaatat tccccacggt
     4801 caccatcttg tcgccaattt gcgcccagat cgcagcattt tcgatggttt gcatataacg
     4861 ggcaggtaac gcggcactaa cgcttgcggt aaacagcatc agcaggatga ataacagctt
     4921 gtttatacat gaaaaaataa ccgagggtga aacaacgctt gtattgtagc aaaagcgcca
     4981 tcataatcgc gggttaatac tcatacaaat tatgtttgag gacaaaaggc ggattttttt
     5041 gttgttggtg ccaaagacta ctcacctccg gcgcacccag ttcgacaata cgctcgcgaa
     5101 actctgaact ggtcatggtt tcacggcaag ccatcatctg ttcaattaac agtaacgtga
     5161 caccagaaat cacttcacaa cgggagtgtt tatggcttaa taatgaagcc acgcggtacg
     5221 gtggcgctcc ggctatatcg gttaagaaga tgacaccctc accagagtcc gcttcatgca
     5281 gagcatcgca catcatgcgg ctgagcatat tagagcttaa tccgcgccag aaattcacgg
     5341 cccggcactg aagaagtgcc ccatgttttt tctccagcgc atccagtatc tcttgcgccc
     5401 ggtcatcgtg acaggtaatt acccaaccta acatcgcctt cctccttagc cgaaatcagt
     5461 ctatcctgac cattgttgat aaagggtgat agccgtcaaa actcctctcc cccgaatcca
     5521 gaggagagga aatagcctta gctacgcaaa ccacgtccac gttggatcag cgaccaacag
     5581 atcaaataaa acaccacaat aaagaccacc agtacgccaa aggtagtggc cagcggcaca
     5641 tcatttatac cgaggaagcc gtagcggaaa ccactgatca tataaacgat tgggttcagg
     5701 tgcgacagcc cttgccagaa cggcggcaac aaagtcaggg agtaaaacac cccgcccaaa
     5761 tacgtgagtg gcgttaacac aaaggttggc accaggctga tgtcatcgaa cgttttggca
     5821 aacacgccgt tcagcaaacc cgcaagggag aatagcaccg ccgtgagcac tagggttaag
     5881 gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa acagtgaaat tgccgtcacc
     5941 agaatgccaa caaacagacc acgcgccacg ccaccgccga catatccggc aataatgacg
     6001 tgagtcggaa ccggcgctac cagcagctct tcaatattac gctggaactt ggcaccaaaa
     6061 aatgatgacg cgacgttagc gtaggcattg gtgatcaccg acatcatgat cagccccggt
     6121 acgatgaact gcatatagct gaagccatgc atatcgccaa tacgcgaacc tatcaggtta
     6181 ccgaagataa taaagtaaag ggtcatggtg atgactggcg gcaccagcgt ctgcacccag
     6241 atacgcataa agcgatggat ctctttcgcc cagatgcttt ttagcgccac ccagtaaaga
     6301 tgcatcatgc gcgatctcct tgtttttcat taaccagtga aacaaacagc tcttccagac
     6361 ggttagcttt gttacgcata cttaatacct gaatgccctg ctcacttaac tgcgtaaata
     6421 cgctgttgat cccctgctca cgcagcactt caacttccag cgtcgcggta tcgaccagtc
     6481 gatactgata gccatcgagc ttcggtaacg ggcttttcgg tgcgagatcg agaataaagg
     6541 tttccgattt cagcttcgcc agcagcgcct tcatcgaggt attttccacc agctcaccgt
     6601 gttgaataat gccgatattg cggcacagca tttctgcttc ttccaggtag tgtgtagtga
     6661 gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa gccccacatt gagcggcgaa
     6721 gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag tagtttaggt tcatgcatta
     6781 acgcacgggc aatcattaaa cggcgcttca tcccgccaga taacatacgc gcacgttcgt
     6841 tgcgttttcc ccataaatcg agttgtttga gatatttttc gctgcggatg tacgcttctt
     6901 tgcgctccac gccgtagtac cctgcctgat tcaccacaat ttgctgcacg gtttcaaacg
     6961 ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg tttagcgttc acgacatcct
     7021 tctcgagatc gtaaccaaat acgctgaccc gcccggaggt tttatttacc agagagctga
     7081 taataccgat agtggtcgat ttcccggccc cgttcggccc gagaagcgca taaaaatcac
     7141 ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac gccgcctgga taggtttttt
     7201 taagctgttg aagttccagt gcaatggtca taaattttta cttaccttac gttcttacac
     7261 tttatctgtg gtttaaatcg tcccggagtt gccctatatt agccaaacgt aattatttgg
     7321 ttacaggtcg ttaacctcca tgaaagacat agatacactc atcagcaaca atgcactatg
     7381 gtcaaaaatg ctggtggaag aggatcccgg gttttttgag aaactggcac aagcgcaaaa
     7441 accgcacttt ctatggattg gatgttccga cagtcgcgtt cctgcagaac gtttaaccgg
     7501 tcttgagccg ggcgaactct ttgttcaccg taatgttgct aacctggtca ttcacaccga
     7561 cctgaactgc ctttccgtga ttcagtatgc agtggatgta ctagaagttg aacacattat
     7621 tatctgtggc cactacggtt gcggcggcgt acaagccgca gttgaaaacc cggaactggg
     7681 gcttatcaac aactggctgc tgcatatccg cgatatctgg ttcaaacata gctcattgct
     7741 cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt gaactgaacg tcatggaaca
     7801 ggtgtataac ctaggccact ccaccattat gcaatcagcg tggaaacgcg ggcagaaagt
     7861 taccattcac ggctgggcct atggcattca cgacggcttg ctgcgtgatc tggatgttac
     7921 tgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac gggatttcca acctcaagct
     7981 gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc aacacatccg gcaacttcac
     8041 acttactcgt ccagcagaat cactttgccg atatacggca gatgacggta acgctgtgcg
     8101 taatcaatgc cgtaacccac cacaaactca tccgggatcg agaaaccgat aaattctacc
     8161 gggacgttca cttcacgacg ggacggttta tccagcagcg tacaaatcgc cagcgacttc
     8221 ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg tattccccga gtcgatgata
     8281 tcttcaacaa tcagcacgtc cttgccacgg atatcttcat ccagatcttt gaggattttc
     8341 acatcacggg tggtggacat gccgctaccg tagctggagg cggtcataaa gtcgacttca
     8401 tgagatacct gaacttcacg gcacaggtcc gccacaaaca taaatgagcc acgcagcagc
     8461 cccaccagca ccatatcgct gccgctgtct ttgtaacgct cagtaatctg acgacccagt
     8521 tcggcgatac gcgctttaat ctccgcttcg gggatcatta cttctacagt atgtttcata
     8581 tctctaacca tatgattaaa aaacaaatca cttcgcacgg gcaggttttg gcaccgcaac
     8641 aaagcgcaag acacgcagta taccagtaaa actattcatt cgcggaatct gttaataaag
     8701 ctcgaattgt gatgacgatc acacatgtta aaacctataa ttaattgcta ctaaaatatt
     8761 aatgaattga aatggtgtct ctttatggca attaacaata caggctcgcg acgattactc
     8821 gtcacgctaa cagtcctttt tgcagcgctt tgcgggctgt atctactcat tggcggaggc
     8881 tggctggtcg cgattggcgg ctcctggtac taccctatcg ctggccttgt gatgctcggc
     8941 gtcgcctgga tgctgtggcg cagtaaacgt gccgcgcttt ggctatacgc cgccctgctg
     9001 ctcggcacca tgatttgggg cgtctgggaa gttggtttcg acttctgggc gctgactccg
     9061 cgcagcgata ttctggtctt cttcggcatc tggctgatcc tgccgtttgt ctggcgtcgc
     9121 ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg tggcactgct gattagcggt
     9181 agtatcctga cctgggccgg atttaacgat ccgcaggaaa tcaacggcac cttaagcgcc
     9241 gatgccacac ctgcggaagc tatctcaccc gttgccgatc aggactggcc tgcctatggt
     9301 cgcaatcagg aaggtcaacg cttttcacct ctgaaacaaa ttaacgccga taacgtccat
     9361 aatctgaaag aagcctgggt gttccgtact ggcgatgtga agcagccgaa cgatccgggc
     9421 gaaatcacca atgaagtgac gccgattaaa gtgggcgaca ccctttacct gtgtaccgct
     9481 caccagcgcc tgtttgcgct cgatgccgcc agcggcaaag agaaatggca ttacgatcct
     9541 aagctgaaaa ctaacgagtc tttccagcac gtaacctgcc gtggcgtctc ttatcatgaa
     9601 gccaaagcag aaacggcttc gccggaagtg atggcggatt gcccgcgtcg tatcattctt
     9661 ccggtcaatg atggtcgact gattgcgatt aacgctgaaa acggcaaact gtgcgaaacc
     9721 ttcgccaata aaggcgtgct caatctgcaa agcaatatgc cagacaccaa accgggtctg
     9781 tatgaaccga cttcgccacc gattatcacc gataaaacca tcgtgatggc aggttcagtt
     9841 accgataact tctcaacccg cgaaacgtct ggcgtgatcc gtggttttga tgtcaacacc
     9901 ggggagctgc tgtgggcttt tgatcccggc gcgaaagatc cgaacgcaat cccgtctgac
     9961 gaacacacct ttacctttaa ctcgccaaac tcctgagcac cagcggccta tgacgcgaag
    10021 ctggatctgg tctatctgcc gatgggcgtg accacgccgg atatctgggg cggtaaccgc
    10081 acaccggaac aggaacgtta tgccagctcg attctggcgc tgaatgccac taccgggaaa
    10141 ctggcgtgga gctaccagac cgttcaccac gacctgtggg acatggatct tccggcacag
    10201 ccgacgctgg cggacatcac cgttaatggt cagaaagtgc cagttattta cgctccggcg
    10261 aaaaccggca acatttttgt gctcgatcgt cgcaatggcg aactggtggt tccggccccg
    10321 gaaaaaccgg tgccgcaagg tgctgcgaaa ggcgattacg ttactccgac ccagccgttc
    10381 tctgaactga gcttccgtcc gaagaaagat ttgagcggtg cggatatgtg gggagccacc
    10441 atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga tgcgctatga aggcattttc
    10501 acgccgccat ctgaacaggg cacgctggtc ttcccgggta acctgggaat gtttgaatgg
    10561 ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg ccaacccaat ggcactgccg
    10621 tttgtttcga aactgatccc acgcggtccg ggtaacccga tggagcagcc gaaagatgcc
    10681 aaaggcacgg gtacggaatc cggcattcag ccacagtacg gtgtaccgta tggtgtcacg
    10741 ctcaacccgt tcctctcacc gtttggtctg ccgtgtaaac agccagcatg gggttatatc
    10801 tcggcgctgg atctgaaaac caatgaagtg gtgtggaaga aacgtattgg tacgccgcag
    10861 gacagtatgc cgttcccgat gcctgtaccg gtgccgttca atatgggtat gccgatgctg
    10921 ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg ccgctacggc agataactac
    10981 ctgcgcgctt acaacatgag caacggtgaa aaactgtggc agggtcgttt accagcgggt
    11041 ggtcaggcga cgccaatgac ctatgaagtg aatggtaagc agtatgtggt gatctccgca
    11101 ggcggtcacg gttcatttgg tacgaagatg ggcgactata ttgtggctta tgccctgccg
    11161 gatgatgtga aataaccata atgcccggag agatccgggc atatttccga atacggtctt
    11221 tttataccgt aaaccctaac atcatccccg tatcttcatg ctccagcaga tggcagtgcg
    11281 ccatataagc atgttctttc ggtgcgtcgt gattaaactt caccagcact tcactgacat
    11341 tgccttctac cttaacggta tctttccagc ccgcgcgatg agccgctggc ggtttgccat
    11401 tttctgacaa gatacggaac tgcgtgccgt gaatatggaa cggatgcagc atcatgtcgc
    11461 ccacgccaga gataacccaa cgttcgtact gccctttcgc cgccgcaaac atcggcttat
    11521 tcatatcaaa cgcctgaccg ttgattttat tggcatggtg gaaatcgaac ttcccgccgt
    11581 ggttcatatg attcatatta ccgtgcccca tatggcccat catctggctg tgatccattc
    11641 cggccatcgc ctgatcgcca tatttctcca ttagcatctg catacccatc atatcgagca
    11701 tcgggtccat agaaagttgc agcttgcgta ccgtcagccc ttccagcgaa ggtaacgcag
    11761 gcaggctact taatgtgtct ggcaaagcac cggaggcact aatagcaatc ggctgaatcc
    11821 gcattaccgg atgcggcttg tcaaacggcg caatcgccat ccccatctgg ctgaccggta
    11881 gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac cagcacttca aaacgctcgc
    11941 ccatcagcac cggcagctcg ctcaccttca ccggttcagg tagcagacca ccgtcgctgg
    12001 caatcacata cagcgggcga ttgtcgctgg tggcgaaatt gagcgagcgg gcattacagc
    12061 cattgagcaa acgcaggcgc agccaaccac gcggggcagc gtgttgtggg tagattgcac
    12121 cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc ggtcatcaca tccagttgat
    12181 aatcaatctg cccatcagcg ttaaattttt tatcctgaac aatcaccgga acatcatcga
    12241 taccccactg ttttggcagc attaatttca ggatctcgtc atcttcaatt accaccagcc
    12301 cagccaaccc catcgccacc tgtcgcccag tcttgccatg ttgatgtgga tggaaccagc
    12361 aagtagcggc aggttgatca acgttcaacg tcaccgagcg cttgccacct ggtggaataa
    12421 ttccctgcgg gccgccgtcg acttcacccg gtacttccag cccgtgccag tgcaacgtcg
    12481 tctcttccgt cagttggtta tagatatcaa ccgttaccgc tttgccgcgc tgtaatttca
    12541 ccgccggccc cagcagattg ccgttatagc cccaggtagt tgcagttttc tcgccaaagg
    12601 tagactgacc tgcgccaata gttaactgaa tgcgattacg ggcatcggtc gtgagcaaat
    12661 cagggatcgg taacgttggg cgttccgccg caaagactgc gcggctccac agcggcaagg
    12721 ctgaagccac acccagcgcg acggaatatt ttaagaaatc acgacgttgc atagttattt
    12781 ccttattctt aagcaggcga aacaaaatca aacgttgagc atagtccttc cccttacggg
    12841 aaggtcaagc cgcagacaca atttaatcga caataatggt cgtcgcctcg ctcgcagtgt
    12901 gctaacgttt atcttcttta agcccctggt agaagcaatg aagacgtttt tcagaacagt
    12961 gttattcggc agtctgatgg ccgtttgcgc aaacagttac gcgctcagcg agtctgaagc
    13021 cgaagatatg gccgatttaa cggcagtttt tgtctttctg aagaacgatt gtggttacca
    13081 gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt ttcgcccagc aaaaccagtg
    13141 ggatctcagt aattacgaca ccttcgacat gaaagccctc ggtgaagaca gctaccgcga
    13201 tctcagcggc attggcattc ccgtcgctaa aaagtgcaaa gccctggccc gcgattcctt
    13261 aagcctgctt gcctacgtca aataatccct gatacctttt tgtagaaata ttgaccgtgc
    13321 atccgcggtc aatgttagct attatgttgc gccctttttt tacgggtgtt aacaaaggag
    13381 gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg ctacacgacc agtttgggca
    13441 gtactttgcg gtagataacg ttctgtatca tgaaaagacc gatcaccaag atctgatcat
    13501 ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat ggcgtagtac aaaccaccga
    13561 gcgcgacgag tttatctatc atgagatgat gacccatgtt ccgctactgg cccacggtca
    13621 cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc atgctgcgtg aagtaacccg
    13681 acataaaaac gttgagtcaa tcacgatggt ggaaatcgat gcgggtgtcg tgtcgttctg
    13741 ccgtcagtat ctgcccaacc ataacgccgg tagctacgac gatccgcgct ttaagctggt
    13801 gatcgacgat ggcgtcaatt tcgttaatca aaccagccag acctttgatg tcattatctc
    13861 cgactgcacc gatcctatcg gtcccggcga aagtctgttc acctcagcat tttatgaagg
    13921 ctgcaaacgt tgcctgaatc ctagcggtat cttcgtcgca caaaacggcg tctgcttttt
    13981 acagcaggaa gaagccatcg acagccatcg caaactcagc cattacttca gcgacgttgg
    14041 cttttatcag gcggcgatcc cgacctatta cggcggtatc atgacttttg catgggcgac
    14101 agataacgac gccttacgcc atctctcaac cgaaattatt caggcgcgtt ttctcgcttc
    14161 tggcctgaaa tgccgttatt acaatccggc aatccatacg gcagcttttg ccttacctca
    14221 gtatctgcaa gatgcactgg cttcacagcc gtcctaagga gaagataaga gattgaaaaa
    14281 actgaaactg catggcttta ataatctgac caaaagtctg agtttttgta tttacgatat
    14341 ctgctacgcc aaaactgccg aagagcgcga cggttatatt gcttatatcg atgaactcta
    14401 taatgccaac cgtctgaccg aaatcctgtc agaaacctgt tccattatcg gggccaatat
    14461 tcttaacatc gcccgccaag attacgaacc acagggtgcc agcgtcacta ttctggtgag
    14521 tgaagaaccg gttgacccga aactcatcga caaaacagaa caccccggcc cactgccaga
    14581 aacggtcgtt gcccatctcg ataaaagtca tatttgcgta catacctacc cggaaagcca
    14641 tcctgaaggt ggtttatgta ccttccgcgc cgatattgaa gtctctacct gcggcgtgat
    14701 ttctccgctg aaggcgctga attacctgat ccaccagctt gagtccgata tcgtaaccat
    14761 tgattatcgc gtgcgcggtt ttacccgcga cattaacggt atgaagcact ttatcgacca
    14821 tgagattaat tcgattcaga actttatgtc tgacgatatg aaggcgctgt atgacatggt
    14881 ggatgtaaac gtctatcagg aaaatatctt ccataccaag atgttgctta aagagttcga
    14941 ccttaagcac tacatgttcc acaccaaacc ggaagattta accgacagcg agcgccagga
    15001 aattaccgct gcgctgtgga aagaaatgcg cgagatttat tacgggcgca atatgccagc
    15061 tgtttaacgg ctctggcgga gctccaggct ccgccagatt tatttacttc tgctgcacga
    15121 aattgcggta agccgccacg acctgcagaa aatcctcaac gccgcatagc gacaggcttt
    15181 cttcgtcgta gtagttcatc ccctcttcca tttcatcgcc agcgaattcc agttgattgg
    15241 cgcgaaccat cacctcttca ccgtccatcc acagggtgta ttcatgccct gcccgttgcc
    15301 aggaccgttc gctacctttc agtgcgtgcg ctgcttgttc cacttcatca agcaaggcca
    15361 ggttttcttt cacctcttca ttaaaccagt gcccgaccac ttcatgcccc atggacatac
    15421 gcacctttac cactccggta atatcgcgca gaaattcgta atccatagtg tgttcctctg
    15481 ctcccggcaa tgcgccgaaa tcgcattgcg ccattgatgt aattatcgca gccttaccgg
    15541 agaaaaacag cggaacggcg atggtttaga aataaaaaat gcccgggaat ctcattcacc
    15601 gggcattgtg tcgtttatgc gcagcgcgtg cgcttactta ttacaccgca gtctggaaaa
    15661 tcaccccatc ggctttttcg gtgtactgag aaagttggtt gaagttcaga taacgataag
    15721 tatcaacggc agttttatct acttgcgcaa cgtaggtctg gtactcttcc ggcgtcggca
    15781 gtttgccaat cagcgccgca acagccgcca gttctgcaga agccaggaag acattcgcgc
    15841 cagtccccag acggttcggg aagttacggg tagaggtgga aaccaccgtc gcaccgtctg
    15901 ctacacgcgc ctggttaccc atacacagag aacagcccgg gatctcgata cgagccccgc
    15961 tcttaccgaa gacgctgtag tagccttctt cagtcaactg tgcggcgtcc atacgggttg
    16021 gcggtgccac ccacaggcgg gtcggcaact ggcctttatg cgcatccagc agtttacccg
    16081 cagcacggaa gtgaccgatg ttagtcatgc aagaaccgat aaatacttcg tcgatcttct
    16141 cgccctgtac cgcagacagc ggacgcgcgt catccgggtc gttcggagca cacaggattg
    16201 gctctttaat atccgccaga tcgatgtcaa tcactgccgc gtattccgca tctgcatcgg
    16261 cttccagcag ctcaggattc gccagccatt tttccatgcc ctgaatacga cgttccaggg
    16321 tacgacgatc gccgtaacct tccgcgatca tccacttcag caggacgata ttagagttaa
    16381 ggtattcgat gattggttct ttgttcagct tgatagtaca accagcggca gaacgctcag
    16441 cggacgcatc ggtaagctca aaggcctgct caactttcag atccggcaga ccttcaattt
    16501 ccagaatgcg gccagagaag atgtttttct tgcctttctt ctcaacggtc agcagacctt
    16561 gtttgatggc atacagcgga atggcgtgta ccagatcgcg cagggtgatg cccggctgca
    16621 ttttgccttt gaagcgcacc agaacggatt ccggcatatc cagcggcatt acgcctgttg
    16681 cggctgcaaa tgccaccaga ccagaacccg ccgggaaaga gataccgatc gggaaacggg
    16741 tatgggagtc acctccggta ccgacggtat ccggcagcag catacggttc agccaggagt
    16801 ggattacgcc gtcacccgga cgcagcgaca caccgccacg gttcataatg aagtccggca
    16861 gcgtgtggtg cgtgttcacg tcaactggct tcggatacgc cgcggtgtgg cagaaagact
    16921 gcatcaccag gtcagccgag aagcccaggc acgccaggtc tttcagttca tcacgggtca
    16981 tcgggccggt agtgtcctgt gaacctacag aagtcatttt cggttcgcag tacgcgcccg
    17041 gacgaatgcc tttcacgcca caggcacggc ctaccatttt ctgcgccagc gagaagccgc
    17101 gatcgctctc agcgacatct ttcgcctgac ggaacacatc actgtgcggc agaccaagtg
    17161 cttcacgcgc tttggtggtc aggccacgcc cgataatcag cgggatacgg ccgccagcac
    17221 gcacttcatc aatcagcacg tcggttttca gttcgaaggt cgccagcagt tcgcctgttt
    17281 cgtggttacg cacttcacct ttgtacgggt aaacgtcaat cacgtcgccc atgttcaggt
    17341 tagagacgtc gacttcgatt ggcagtgcac ccgcgtcttc catcgtgtta aagaagatgg
    17401 gtgcaatttt accgccgagg cacaaaccac cgccgcgttt gttcggcaca tgtggaatat
    17461 catcgcccat aaaccacaga acggagttcg tggcggattt acgcgatgaa cccgtaccca
    17521 caacgtcacc gacgtacgcc agcgggaaac ctttctgttg cagagcttca atctgtttga
    17581 tcgggccaac aacacctggc tggtctggct caataccttc acgggcgttt ttcagcatcg
    17641 ccagcgcgtg cagtgggata tccgggcgcg accacgcatc cggtgccgga gaaaggtcat
    17701 cggtgttggt ttcgccagtg actttgaaga cggtaacggt cagtttttca gccagcgccg
    17761 ggcgattcag aaaccattcg gcatccgccc aggactgcat tacctgcttc gcatattcgt
    17821 tgcctgcttt cgctttctct tctacgtcat agaagttatc gaacatcagc agtgtgtgag
    17881 aaagtgcttt ggcagcgatc ggtgccaatt tggcatcatc cagcgcgtcg atcagcggat
    17941 gaatgttgta accaccctgc atggtgccca gcagttcgat ggctttttcc ggagtcagca
    18001 gaggggattt ggcttcgcct ttcgcgatag cagccaggaa gcctgctttg acataggcgg
    18061 cttcatcgac gccaggggga acacggttgg ttaacagatc taacaggaat tcttcttcgc
    18121 ccgcgggcgg gtttttcagc agctctacaa gtgcggccat ttggtttgca tccaggggtt
    18181 tgggcgcaat cccctcagcg gcacgctcag ctacgtgctt acggtattct tctagcacga
    18241 cggttctcct cgctctcatt gtcatagtgc ggcaggcgat tctcttcacg ctcctgtgag
    18301 acagcagttt gtagggtaaa tgcccagtac cgcaacgggc agaatagcag gattttgacg
    18361 acgtgttaat ctgtttacaa aaaagcaaca taaaaaagtg gctgaatcgt taaggatggt
    18421 ctagagattg tttccctccg taaaaacttc gttaattcgc atggtgataa tcacctttct
    18481 caacgaatcc caagattagt caaaatttaa actaccgcct ctttatactc ggattaacag
    18541 cacctgcggg tggcagttcg ccgaccattg cgatttcctt gagatccaaa ttatgaaaat
    18601 gactttgccg tttaaacccc atgtgctggc actaatttgc agtgccgggc tttgtgccgc
    18661 ctctgccggg ctatatataa aaagccgcac agtggaagcg gctgtggaaa cgcaatcgac
    18721 acaactggct gtgtctgacg ctgccgcggt tacgcttcct gcaacggttt ccgcgcctcc
    18781 cgtaacaccc gccgtcgtta aatccgcatt cagcactgca caaatagatc aatgggtcgc
    18841 gcccgtcgcg ctgtatcccg acgccctact ttcgcaggtg ctgatggcat caacctatcc
    18901 ggcaaacgtt gctcaagcag tgcaatggtc gcacgataat ccacttaaac aaggcgatgc
    18961 tgctattcag gcggtatctg accagccgtg ggacgccagc gttaaatcac tggtggcctt
    19021 tccacaattg atggcattga tgggcgaaaa cccgcaatgg gtgcaaaacc tgggcgatgc
    19081 ttttctggcg cagccgcagg acgtgatgga ctcggtacag cgattgcggc aactggcgca
    19141 acaaaccggt tcgctgaagt catcaaacga acaaaaagtt attaccacaa cgaagaaagc
    19201 tgtaccggta aaacagacag tcactgcacc cgtcatacca tccaataccg ttttaactgc
    19261 cagccccgtc attacagagc ctgcaacaac cgtcatttcc attgagcccg ccaatcctga
    19321 tgtggtctat attcccaact acaacccaac cgtggtttac gggaactggg ccaatactgc
    19381 gtatccgccg gtttatctgc caccaccagc cggagaaccg tttattgaca gctttgtgcg
    19441 cggattcggc tatagcatgg gtgttgctac cacgtacgca ctattcagca gcatcgactg
    19501 ggatgacgac gatcatgacc atcatcatca tgacgatgat gattatcatc accacgatgg
    19561 cggtcatcgt gacggtaatg gctggcaaca caacggcgac aacatcaata tcgacgtcaa
    19621 caatttcaac cgtatcaccg gtgagcatct tactgataag aatatggcat ggcggcacaa
    19681 tccaaactac cgtaatggta tgccctatca tgatcaggat atggcaaagc ggtttcatca
    19741 aaccgatgtc aacggcggaa tgagtgccac gcagctacct gctccaacac gcgacagcca
    19801 gcgtcagacg gcagcaagtc agtttcagca acgaacacac gccgcccccg tcattacacg
    19861 agatacccaa cgtcaggcag cggcacagcg gtttaatgaa gcggagctat gacgacttcc
    19921 gcgacttcag ccgtcgccaa cccctgaccc agcaacaaaa ggacgccgct cgtcagcgtt
    19981 atcagtcagc ttctcctgag cagcgccagg tgtttaagga aaaagtacag cagcgcccac
    20041 tgaaccaaca gcaacgtgat aacgcccgcc agcgtgttca atcagcatca cctgaacaac
    20101 gtcaggtttt tcgggagaga gttcaggaga gccgcccaca acgtctaaac gacagtaacc
    20161 gtactgcaag attgaataac gatcaacggt cagcagtacg cgaacgtctc tctgagcgcg
    20221 gagcaaggcg actggaaagg taaattacag acgtaaaaaa agcggcgtgg ttagccgctt
    20281 ttttaattgc cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg
    20341 caggtcggta atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt
    20401 cgggtgcgca tggatggtca gcgcgatgtc ttcagcgtca caacccattt cgattgccag
    20461 gccgatttca cccagcagct cgccgccgtt ggtaccgaca atcgcaccac cgatcacacg
    20521 gtgagattct ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcgg aagcgatagc
    20581 acgaccagaa gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc
    20641 ttctttctca gtcagaccta cccatgcaac ttctggttcg gtataggcga tagacgggat
    20701 aactttcgga tcgaagtagt gttttttacc ggcgataact tcagcggcaa cgtgaccttc
    20761 gtgaacacct ttgtgtgcca gcatcggctg accaacgata tcgccgatag caaagatgtg
    20821 cggtacgttg gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt ccacttccac
    20881 gccagctttg cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac
    20941 ggcgtcgtaa cgctgcggtt cagcgggtgc ttttttgcct tccatcgtca cataaatacc
    21001 gtcttctttc gcttcaacag cggtaacttt ggtttccagc atcaggttga atttcttgct
    21061 gatacgcttg gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa
    21121 catttcaacc acgtcaatct gtgaacccag cgcgtggtat acggtgccca tttccagacc
    21181 gatgataccg ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc
    21241 agtggagtcc cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga
    21301 acccgctgca atgatcgcgt tgtcgaagtt gatcacggtt ttaccgttct caccttcaac
    21361 ttccagggtg ttagccccgg taaatttacc cagaccgttg accactttga ctttgcggcc
    21421 tttcgccata ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg
    21481 aatcttgtca atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt
    21541 ggcttcttcg ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt
    21601 caggcaaaca ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc
    21661 agcgcaacgg aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt
    21721 tttgatttca gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt
    21781 ctttcaggcc gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc
    21841 ccctggggat tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag
    21901 taatacccta accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa
    21961 tttttaaaca acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg
    22021 agattaccag aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg
    22081 aatgtcagac agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat
    22141 cacgcggtgg tcgaaggaga gagaaagcgg cagcatcaga cgcggcacga actctttacc
    22201 attccacacc ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt
    22261 cacaatcggc gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc
    22321 gccctgcatt tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag
    22381 ctcgcgagac agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag
    22441 accgttcggg gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg
    22501 accgtcttcc gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc
    22561 tttcatgatg aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc
    22621 gttctgctgt ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac
    22681 atgcgggatc atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc
    22741 cagttccact tcttcgattt caccaaactt gctgaagtcc accttcggcc acggcagcat
    22801 gcctgggata ccgccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt
    22861 cacgtaagcc tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt
    22921 cgccaggtta acaccaaact cgcgtgccag acggcggatc agcggagtcg cgtgaacata
    22981 agcgtcgttt tcagcaaatt cagatttgcc ttccgctttc gcagctggtg ctgctgccgg
    23041 ggcttcagct tttgctgccg gtgccggcgc tgccgcttcc tgtttcgcag gagctgccgc
    23101 aggcgctgcg ccttcaactt cgaagatcat aatcagcgag ccagttttca ctttatcgcc
    23161 aacgttgact ttcagttcct tcacgacgcc tgcaaacggc gccggaactt ccatagaagc
    23221 tttgtcgcct tctacggtga tcagtgactg ttcagcggca actttgtcgc ccactttcac
    23281 catcacttca gtcacttcaa cttcgtcacc gccgatatcc ggaacgttaa cttctttcac
    23341 gccagctgct ggtgcagggg ccgctgccgg agccgcttcc tgtttagccg ccggagctgc
    23401 cgcgcctgct tcacccgcga cttcgaagac cataatcagc gagccggtag acactttgtc
    23461 acccacgttc actttgatct ctttcacggt gccagcaaac ggagccggaa cttccataga
    23521 agctttatcg ccttctacgg tgatcagcga ctgttcagct tcaactttat cgccaacttt
    23581 caccaggatt tcggtcactt caacttcgtc gctgccgata tccggaacgt taacgtcttt
    23641 tgccgccgca gccgcgggtg ctgctgccgg agctgcttct ttcttctctt ctgcctgagc
    23701 aggtgcagcg tctgctgcac cgtcggcgga atcgaaaatc ataatcagtg cgccggtctg
    23761 ggttttatcg ccaacagaga ctttgatctc tttaacgata cccgcctgcg gagacggaac
    23821 ttccatagag gctttgtcgc cttctacggt gatcagcgac tgctcggctt caactttgtc
    23881 gcccactttg accaggatct cggtgatttc aacttcatca gccccgatgt ccggtacttt
    23941 gatttcgata gccattattc ttttacctct tacgccagac gcgggttaac tttatctgca
    24001 tcgatgttga atttggcgat tgcgtcagca accactttct tatcgatttc gccacgttta
    24061 gccagttcgc ccagcgccgc aaccacgaca taagaagcat caacttcgaa gtggtgacgc
    24121 aggttctcac ggctgtcgga acgaccgaag ccatcagtac ccagtacgcg gtagtcgtca
    24181 gccggtacgt aagtacggac ctgctcagcg aacagtttca tatagtcggt agatgccact
    24241 gccggagcgt cgttcatcac ctgagcgata tacggaacgc gcggagtttc cagcggatga
    24301 agcatgttcc agcgttcaca atcctgacca tcacgcgcca gctcggtgaa ggaggtcacg
    24361 ctataaacgt cagaacctac gccgtaatct ttcgccagga tctctgccgc ttcacggacg
    24421 tgacgcagga tagaaccgga gcccagcagc tgaactttac ctttgctacc ttcaatggtt
    24481 tcgagtttgt agataccttt acggatacct tcctcagcac cttccggcat tgccggcatg
    24541 tggtagtttt cgttcagcgt ggtgatgtag tagtaaacgt tctcttgttt ttcaccgtac
    24601 atacgctcca gaccgtcatg cataatgaca gcaacttcgt aagcgtaagc cgggtcgtaa
    24661 gagatacagt tcggaatagt cagcgactga atgtggctgt gaccatcttc atgctgcaga
    24721 ccttctccgt tcagggtggt acgaccggaa gtaccgccga tcaggaagcc acgcgcttgc
    24781 tggtcgccag ccgcccagca cagatcgcca atacgctgga agccgaacat cgagtaatag
    24841 atgtagaacg ggatcatcgg cagattgttg gtgctgtaag aggtcgccgc tgccagccag
    24901 gaacaacctg cgcccagctc gttgatccct tcctgcagaa tctgaccttt ctcgtcttct
    24961 ttatagtaag caacctgctc gcggtcctgc ggggtgtact gctgaccgtt cgggctgtaa
    25021 ataccaatct gacggaacag accttccata ccgaaagtac gcgcttcgtc ggcgatgatc
    25081 ggtaccagac gatctttgat cgacttgttc ttcagcatta cgttcagggc acgaacgaaa
    25141 gcgatagtgg tagagatctc tttgctctgc tcttccagca gcgcgccgaa gtcttgcagg
    25201 ctcggcagct caagcttctc ggtgaagttc ggctgacggc ttggcagata accgtgcagt
    25261 ttctgacgct gtgcgtgcag ataggtatgc tcttcagaac cttccgggaa ggtgatgtac
    25321 ggcagttttt cgatatctgc atcagacacc ggcacattga aacggtcgcg gatgtgacgc
    25381 acgccgtcca tgttcatttt cttaacctgg tgcgcgatgt ttttaccttc agccgcgtcg
    25441 cccatgccgt aacctttaat ggaatgagca aggattactg tcgctttgcc tttggtttcc
    25501 tgcgctttct tgaatgcagc gtagattttc ttcggatcgt gaccaccacg gttcagtgcc
    25561 cagatctgct cgtcagtcca gtctgcaacc agtgctgcgg tttcaggata tttaccgaag
    25621 aagtgttcac gaacgtacgc accatctttc gatttgaagg tctggtagtc gccgtcaacg
    25681 gtttcgttca tcagctggat cagtttaccg ctggtatctt tacgcagcag atcatcccaa
    25741 cggctacccc acatcacttt gatcacgttc cagccagcac cttcgaagat gccttccagt
    25801 tcgttgatga tcttgccgtt accggtgacc gggccgtcaa gacgctgcag gttacagttg
    25861 ataacgaaga ccaggttatc cagtttttca cgggtagcga tggtgatcgc acctttagat
    25921 tccggttcgt ccatttcacc gtcgccgagg aacgcgtaaa cggtctgttt agaggtatct
    25981 ttcaggccac ggtgttccag atatttcagg aatttagcct ggtaaatagc accaatcggc
    26041 cccagcccca tagatacggt cgggaactgc cagaattccg gcatcagttt cgggtgcgga
    26101 taggaagaga ggccattgcc gtgaacttcc tgacggaagt tatccagctg ctcctgagtc
    26161 agacgacctt ccaggaaagc acgtgcgtac acgcccgggg agatgtggcc ctggaagtaa
    26221 accaggtcgc cgccatcctg ctcgttgcgt gcacggaaga agtggttaaa gcacacatca
    26281 taaatggttg cggaagactg gaaggacgcc atatggccgc ccagttcgag gtcttttttc
    26341 gacgcacgca gcaccgtcat gatggcgttc cagcggatag ctgaacgaat acggcgttcc
    26401 agttccagat tacccggata ctccggttgt tcttcaacgg ggatggtgtt gatgtagttg
    26461 ctgatacctg tgcctgcggc tacgtttaca ccgcctttgc gggcttcagc aagcagttgg
    26521 tcgatcagat actgagcacg ctcaacacct tcttcacgga tgaccgattc gatcgcctgg
    26581 agccagtcgc gagtttcgat cggatccacg tcatttggga aacgttctga catgggttat
    26641 tccttatcta tctaataacg ttgagttttc tggaacctgt cccattgaac tctcgccgga
    26701 aagctcaata agacaggttc tacgtttagt tgccgcgctt ttatatgcgc ttgatttaca
    26761 acatcttctg gataattttt accagaaaaa tcactaattc tttcgttgct ccagacgacg
    26821 cagagaacgc tcacggcggc tctcttcacg acttctgtcg agcaaaattt cttcgataaa
    26881 ggccagatgg cgatgcgatg cttcgcgcgc ttcttccggc ttaccggcca taatcgcttc
    26941 aaatatgcgg gtgcggtgac tactcaccag cggcagcatc tcgcgacgcg aatagagcaa
    27001 ttcgaagttc tggcggacat tctgggccaa catcggctcc atacacctta gcagatgaag
    27061 cagaaccaca ttgtgggccg cttcagtgac agcaatctga tactggagta cggcgtttga
    27121 ttccgcgtcc agatcgccag actgctgcgc cagttctatg gcgtgatgga gttcacagat
    27181 gcgttccttg tcttcatcgg tactacgcag cgcagcgtaa taagcggcga taccttccag
    27241 ggcgtgtcgt gtttcgagca agtcatactg tgactcagga tggtcggaga gcagctccac
    27301 cagcggatcg ctgaagcttt gccataggct gctctggaca aaagtgccgc caccctggcg
    27361 acgaagcaac aagcccttcg cttcgagacg ttgaatcgcc tcacgcaagg agggacggga
    27421 gacgtcaaac tgttttgcca gttcgcgttc cggtgggagt ttttcgcccg ggcggagagt
    27481 gccttcgagg atcaaaaact ccagttgctg ctcaatcaca tcggagagtt ttggttggcg
    27541 gattttgctg taggccatga gttcctgtct taagccactt gccgaagtca attggtctta
    27601 ccaatttcat gtctgtgacg ctaaagtaac aaagtattca ccttatgtcc atacaggttt
    27661 tgattgaaat catgaaactg tgcacatttt aacaacttga catatataac gtttcaaagt
    27721 tgtaactatg cacaaatgta gactttacgt aggaaaggga ttttttaacg attattaaac
    27781 tataaaaatc cgaattgaac cgattcactt accaattttg tgatttttaa ttcaattaaa
    27841 acgaatttaa attcattcta catattgaga ggggttgagg ctgagcttta caaacggttt
    27901 ctttttaagc aactcatctt caaccatgca taaagcgggt gcattcgctg ccgcatacca
    27961 ttattcttga tctgacggaa gtctttttgt aacaattcaa acttctttga tgtaaacaaa
    28021 ttaatacaac aaacggaatt gcaaacttac acacgcatca ctgcgtagat caaaaaaaca
    28081 accaccgcac gaggtttcat gatggaaggt caacagcacg gcgagcagct aaagcgcggc
    28141 cttaaaaacc gccatattca gcttatcgcg ctgggtggcg cgatagggac cgggttattc
    28201 ctgggtagcg cctccgtaat acagtccgca gggccaggga ttatcctggg ttacgccatt
    28261 gctggtttta tcgcctttct gatcatgcgt cagctgggtg aaatggtggt cgaagaacct
    28321 gtcgcaggct cctttagcca ctttgcttat aaatactggg gcagttttgc cggtttcgcc
    28381 tctggctgga actactgggt actgtacgtt ttagttgcca tggctgagct gactgccgtg
    28441 ggtaaataca ttcagttctg gtatccggaa atccccacct gggtttctgc cgccgtattc
    28501 tttgtggtga ttaacgccat caacctgacc aacgttaaag tgtttggcga gatggagttc
    28561 tggtttgcca ttatcaaagt tatcgcggtg gtagcgatga tcatcttcgg cggctggctg
    28621 ctattcagtg gcaacagcgg cccgcaggcg accgttagca acctgtggga tcagggtggt
    28681 ttcctgccgc acggcttcac cgggctggtg atgatgatgg cgattatcat gttctcgttc
    28741 ggtggtctgg aactggtggg gatcaccgca gcagaagctg ataacccgga gcaaagtata
    28801 ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt tctatattgg ttcgttagcc
    28861 gttctgctct cactgatgcc gtggacccgc gttaccgccg ataccagtcc gtttgtgctg
    28921 atcttccacg agttaggcga tacctttgtg gcgaatgcgc tgaacatcgt ggtactgact
    28981 gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca gccgtatgct gtttggtctg
    29041 gcacaacagg gtaatgcgcc aaaagcgctg gcgtctgtcg ataaacgtgg tgtaccagta
    29101 aataccattc tggtgtctgc actggtaacg gcgttgtgcg tactgattaa ctaccttgcc
    29161 ccagagtccg ctttcggact gttaatggcg ctggtggtat ctgcactggt aatcaactgg
    29221 gcgatgatta gcctggcgca tatgaaattc cgtcgcgcca agcaggaaca aggcgtggta
    29281 actcgcttcc ctgctctgct ttatccgctg ggtaactgga tctgcctgct gtttatggcg
    29341 gcggtactgg tgattatgct gatgacccca ggaatggcga tttcggtata cctgatcccg
    29401 gtatggctga tcgtgttagg tatcggctat ctgtttaaag agaaaaccgc caaagccgta
    29461 aaagcgcatt aatctctcta cgccctcacc cgtacagggt gagggcaata atctttacac
    29521 caacgccccg taaatcgtca gtagtgcaat caccaccacg accacgaacg aggttttctt
    29581 cgccattgaa accgctgcct tcggcgtctc caccttatcg acatgcggtt cacgcgccag
    29641 agagaactgc gccagacgcg ttaacacctg atactgcgaa gtatggaagt cacccagcga
    29701 agcaaaccag gccggtaacg ctttctcccc atgaccgatc aaggcatata ccacacccgc
    29761 aagacgaacc ggcacccaat ccagtacatg aagcacggca tcaatgccgg actgtaaacg
    29821 atgatgcggc gtctggtatc gcgccagcca gtattgccat gcacgcaaaa acgcataccc
    29881 catcagcgta acgggtcccc agattccccc cacaatcaac cagaacagcg gagcaagata
    29941 aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca cgcaaatact cacgttcatc
    30001 gcagcctgcc gggacgccgt gaatcatggt gagttcgcca gccatcgtga cacgggcatg
    30061 gctatcatta cgtgaagcag ctgtcagata agcatgataa tgaagacgaa ctttacctgc
    30121 gccaatacac agcaaaccaa tcagcagcca caccagcagc gtgggaacgt tgaacaatac
    30181 tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc gcaataatgg tcatgcctaa
    30241 cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac gcttcaagac gatgatcaag
    30301 ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt aacaccagta aggttgtaaa
    30361 tagcgtcatg ttgtctcctt gctgaccagc gcacgaaacc gcgcccaatc aaatgcaggg
    30421 ccgggatcgg ttttccgatc cggcgcaata tcacaatggc ccgtcatgtt attagcgata
    30481 tctggatagc gatcaatcag tgcccgcgta accgccgcaa gctgttgata ctgcgcatcg
    30541 gtatacgcca gcgtatcggt gccttcaagc tcaatcccaa tagaaaaatc attgcagcgt
    30601 tcgcgcccct gatactgaga gactcccgca tgccatgcac gtttatcgaa aggaacatac
    30661 tggactattt caccatcacg gcgaatcaaa cagtgagcgg agacgcgcaa atgggcgatc
    30721 tcagcaaaga aaggatgtgc ctgcggatca atagttccag tgaataatgc gtcgatccac
    30781 ggaccgccaa actcgcctgg cggcaggcta atattgtgca ccaccagcag ggtgggtgtt
    30841 tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc ggcgcgcgcc aaccagccac
    30901 ccctgttcta acaacatgca ggatctcctt atatgtggtg ctaatacccg gttcagagta
    30961 gcatgtttct accttatgat tcgttagcta tctggagttt taacatgccg cctcgccgct
    31021 ataaccctga cacccgacgt gacgagctgc tggaacgcat taatctcgat atccccggcg
    31081 cggtggccca ggcgctgcgg gaagatttag gcggaacagt cgatgccaac aatgacatta
    31141 cggcaaaact tttaccggaa aactctcgct ctcatgccac ggtgatcacc cgcgagaatg
    31201 gcgtattttg cggcaaacgc tgggttgaag aggtgtttat tcaactggca ggcgacgatg
    31261 tcaccataat ctggcatgtg gatgacggcg atgtcatcaa cgccaatcaa cccttgttcg
    31321 aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg cactgcgctt aattttgtgc
    31381 aaaccctttc aggagttgcc agtaaggtac gccactatgt cgaattactg gaaggcacca
    31441 acacgcagtt gttggatacg cgcaaaacct tacctggcct gcgttcagct ctgaaatacg
    31501 cggtactttg cggcggcgga gcgaatcacc gtctggggct ttctgatgcc ttcctgatca
    31561 aagaaaatca tattattgcc tccggctcag tgcgccaggc cgtcgaaaaa gcgtcctggc
    31621 tgcacccgga tgcgccagta gaagtcgaag tagagaatct ggaagaactt gatgaagccc
    31681 tgaaagcagg agccgatatc atcatgctgg ataacttcga aacagaacag atgcgcgaag
    31741 ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc tggcaacgtc actgacaaaa
    31801 cactgcgcga atttgccgaa acgggcgtgg actttatctc cgtcggtgcg ctaactaaac
    31861 acgtacaagc actcgacctt tcaatgcgtt ttcgctaatt gctgacagat ttatccgctc
    31921 catcgcgggc ggataatctc cccttcccca actttcttcg taacgcctcg caaatttctt
    31981 gtctttcgct gcaacgaaat aaaaaacctc ggaagctgct ttcccgtttc gttttttgcc
    32041 cctcgcttgt gctgatggcg tttgccaaag tagcgccaac caaatcaagg agcgaaacag
    32101 atggacaagc aacgcggttt tacacttatc gaactgatgg tggttattgg catcattgcc
    32161 attttaagcg ccattggtat tcccgcttat caaaactacc tgcgcaaagc tgcactcacc
    32221 gacatgctac aaacctttgt gccttaccgt accgccgtag agttgtgcgc gctggaacat
    32281 ggtggatt
//
LOCUS       DAJTNA010000025        31370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTNA010000025 DAJTNA010000000
VERSION     DAJTNA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31370
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.025"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(18..3299)
                     /locus_tag="OO161_001005"
     CDS             complement(18..3299)
                     /locus_tag="OO161_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5462161.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            3417..4610
                     /locus_tag="OO161_001006"
     CDS             3417..4610
                     /locus_tag="OO161_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5462162.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCQETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4674..5927)
                     /gene="glyA"
                     /locus_tag="OO161_001007"
     CDS             complement(4674..5927)
                     /gene="glyA"
                     /locus_tag="OO161_001007"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5462163.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6255..7445
                     /gene="hmpA"
                     /locus_tag="OO161_001008"
     CDS             6255..7445
                     /gene="hmpA"
                     /locus_tag="OO161_001008"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5462164.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVERIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7490..7828)
                     /gene="glnB"
                     /locus_tag="OO161_001009"
     CDS             complement(7490..7828)
                     /gene="glnB"
                     /locus_tag="OO161_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5462165.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7889..9223)
                     /gene="glrR"
                     /locus_tag="OO161_001010"
     CDS             complement(7889..9223)
                     /gene="glrR"
                     /locus_tag="OO161_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5462166.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9213..9926)
                     /gene="qseG"
                     /locus_tag="OO161_001011"
     CDS             complement(9213..9926)
                     /gene="qseG"
                     /locus_tag="OO161_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5462167.1"
                     /translation="MRHIFQRLLLRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10090..11517)
                     /gene="qseE"
                     /locus_tag="OO161_001012"
     CDS             complement(10090..11517)
                     /gene="qseE"
                     /locus_tag="OO161_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5462168.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12075..15962)
                     /gene="purL"
                     /locus_tag="OO161_001013"
                     /gene_synonym="purI"
     CDS             complement(12075..15962)
                     /gene="purL"
                     /locus_tag="OO161_001013"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5462169.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16220..17776
                     /gene="mltF"
                     /locus_tag="OO161_001014"
     CDS             16220..17776
                     /gene="mltF"
                     /locus_tag="OO161_001014"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5462170.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGV
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEDIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17773..18309)
                     /gene="tadA"
                     /locus_tag="OO161_001015"
     CDS             complement(17773..18309)
                     /gene="tadA"
                     /locus_tag="OO161_001015"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5462171.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18334..18969)
                     /gene="yfhb"
                     /locus_tag="OO161_001016"
     CDS             complement(18334..18969)
                     /gene="yfhb"
                     /locus_tag="OO161_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5462172.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19178..20026
                     /locus_tag="OO161_001017"
     CDS             19178..20026
                     /locus_tag="OO161_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5462173.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20082..20342
                     /gene="yfhL"
                     /locus_tag="OO161_001018"
     CDS             20082..20342
                     /gene="yfhL"
                     /locus_tag="OO161_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5462174.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20536..20616)
                     /gene="shoB"
                     /locus_tag="OO161_001019"
     CDS             complement(20536..20616)
                     /gene="shoB"
                     /locus_tag="OO161_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5462175.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21036..21416)
                     /gene="acpS"
                     /locus_tag="OO161_001020"
     CDS             complement(21036..21416)
                     /gene="acpS"
                     /locus_tag="OO161_001020"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5462176.1"
                     /translation="MAILGLGMDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21416..22147)
                     /gene="pdxJ"
                     /locus_tag="OO161_001021"
     CDS             complement(21416..22147)
                     /gene="pdxJ"
                     /locus_tag="OO161_001021"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5462177.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22159..22887)
                     /gene="recO"
                     /locus_tag="OO161_001022"
     CDS             complement(22159..22887)
                     /gene="recO"
                     /locus_tag="OO161_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5462178.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSSITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22899..23804)
                     /gene="era"
                     /locus_tag="OO161_001023"
     CDS             complement(22899..23804)
                     /gene="era"
                     /locus_tag="OO161_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5462179.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23801..24481)
                     /gene="rnc"
                     /locus_tag="OO161_001024"
     CDS             complement(23801..24481)
                     /gene="rnc"
                     /locus_tag="OO161_001024"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5462180.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPCVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24754..25728)
                     /gene="lepB"
                     /locus_tag="OO161_001025"
     CDS             complement(24754..25728)
                     /gene="lepB"
                     /locus_tag="OO161_001025"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5462181.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25744..27543)
                     /gene="lepA"
                     /locus_tag="OO161_001026"
     CDS             complement(25744..27543)
                     /gene="lepA"
                     /locus_tag="OO161_001026"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5462182.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27741..28220)
                     /gene="rseC"
                     /locus_tag="OO161_001027"
     CDS             complement(27741..28220)
                     /gene="rseC"
                     /locus_tag="OO161_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5462183.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28217..29173)
                     /gene="rseB"
                     /locus_tag="OO161_001028"
     CDS             complement(28217..29173)
                     /gene="rseB"
                     /locus_tag="OO161_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5462184.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29173..29823)
                     /gene="rseA"
                     /locus_tag="OO161_001029"
     CDS             complement(29173..29823)
                     /gene="rseA"
                     /locus_tag="OO161_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5462185.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29856..30431)
                     /gene="rpoE"
                     /locus_tag="OO161_001030"
     CDS             complement(29856..30431)
                     /gene="rpoE"
                     /locus_tag="OO161_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5462186.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30428..30583)
                     /gene="rseD"
                     /locus_tag="OO161_001031"
     CDS             complement(30428..30583)
                     /gene="rseD"
                     /locus_tag="OO161_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5462187.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30839..>31370
                     /locus_tag="OO161_001032"
     CDS             30839..>31370
                     /locus_tag="OO161_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5462188.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTR"
BASE COUNT         7497 a         8399 c         7919 g         7555 t
ORIGIN      
        1 attacagtta atagggttta gtgaacgtag ttaaaaataa aaggcatcac agtggtgaat
       61 aatgccgctt tcggccaggc cggattaatc gccgtcaatt cagcgcgagc agactcatat
      121 tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca ccatcaggca tttttcttta
      181 tgctgctgtt gtaaatcgcc atacagcgat aacaggtcag gttgtgaaac agcaaagaaa
      241 tccgcctcaa tactggtttt cgccatctct tgcgcccact gtttcatttc actaaacagt
      301 tgctgtgcgg tttgctgttc acccagcagt cgcagcgcca ttccttgcca gaagagataa
      361 tcaaccggct gatcgttgta ataactgtgg atgttaatgg tgcgatcgcc ggtcgccgcc
      421 agacgtaaac aacgcatcgc ttcagtttca tcgccctgcg cgttggcgca tatcgcctgc
      481 cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc cttcgcttaa attctccgga
      541 taatgcagcg cggcatgaag cagttcgctg gcctgctgcg gctctctggc atcaagatgc
      601 tgccaggcgc gtaataactg gttgaggata aactgactgg tgaccttccc ttccccgcct
      661 tcccacgggt ggaatttacg cgtggcgaga atgtccgccg ctttgtctgc ctgacccgtg
      721 agatgccaca aattgagcag ttctgcggtc atgtcgtcgc gtttcagcgc aatttccaga
      781 ttattttcca gacgcgccag tcgtttctcc ggtgtggctc cacttaactt atcaagcaaa
      841 tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct gataagcatt atcaagataa
      901 cgcgcggcca gctcataatc gtgttgctta ttccacgcat ggatcgctaa cccgcgccag
      961 ccgtcggcaa actccggcga catctctacg caacgttgcc aaaaggcaat ggctttgttg
     1021 tagctacgtt tgttgtagta gaagcaggcc agtaaatggc gagcaaacca gcactcttca
     1081 atactctcca gcgccgccac ttcttccagc gtattcggga aacggacaaa ctgcgggaag
     1141 acatcaatgg cttttgcgac cagttcgcca cgttcggctt tcggcagcaa gctggcttgc
     1201 aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca gcatctctgc cgccagggtg
     1261 ggcattcccc agttaatcag ttgcccggcg gtcatcaggg cgttaacgtc acgtccctga
     1321 cacagaccgc gccactgcgc gagagctgat tcgctacgac catcgaacca gttcagccac
     1381 cacagagtgg cgttcagcgg ataatcgcgc agcagtttct cgcgctgcac acgcgcgttg
     1441 tcctgacgac cacttaacac cagcagcaga ttatgcaggc aaagcacttc ctgattggtt
     1501 gggcaggcgc gaagactttg ttggcaaaaa tccagaccag cgtcgaagtt accattacgc
     1561 gccgccagtc gtgccaggcc ataatagcca ccggctttgc tgttgccgct ccagaccgca
     1621 cgccagaaat cctcttcggc ttgttgatat tgtccctgac gttcgtaagc actggcgcga
     1681 atcaaactcg cctgtccgca ctgcggattt ttgttcagcg catgtgcgcg tttcagagcc
     1741 tgagtggcat acgccaccgc ttgcgggaaa tcggcgcggt tatattccag catcgccagc
     1801 gccaggttac agcggtaatc cagcggatcc agcgccacgc cgcgcaggta gtaatcgaac
     1861 ggtgaacggc tggcgtgatg atattgctcc agatgctgac cgataaacca ggcttcatct
     1921 gtactggtaa tgtcttgtgc tgccagtggc gctttggcga cgtccagcaa cggcaactct
     1981 tgcggctgat gttcctgata actcagtaca atattgccgt cggcatcgga gagctcaatg
     2041 gtcagccttt caggattgat accgtgcaac acgccctgga tggcggtcgc aggcatcagt
     2101 gccacggcat catcaagtaa cgcgttgcat ttgccgattt cgcggatcgc caggcggtat
     2161 ccgttcaacg gagagatggc atacagcccc cactcaatcc cccgcttact acgctggagt
     2221 tttatcaccg catcgcggga ggcattttgc accatgccca aagaatgata aggcaggaaa
     2281 tactgctcaa aacgcttctc ttcgtaagca tcaagccagg taaaatcggg ctggttatcg
     2341 gcaaaaatac cggtcatcag ttcgatatac gggccgttat tgtcggtcag actcttatcc
     2401 cacgcctggc caaattcact gtgtccccaa ctccactgtt ttttacctgg cgcaatatgg
     2461 tggttggcaa cgtgcagcaa accgccatct tcatcgtgac accacgcgcc gacaaaatcg
     2521 tactgcgatt tttcggccat atatgaggtt ggaacaggca catttttata gcgagaaatg
     2581 tccactccgg cggagtagtc cactttgtag taagtgccgg tggcgatggg gaaagcggag
     2641 acggcccgtt tgccgtgatc aaacaccgca gtcacatccg gtgggaaaac gctctgatgc
     2701 ccttcccccc ctttcactgc cgggttggcc caccacaaga aatgacgcgg cgtggcgtta
     2761 ccgttataga cgcggctggc gatttccagc gccgcccggt cagggcgcag ggtgaaacct
     2821 gtcatcacct gtaaaccatg catcggctcc gtttcgccta cccacaccgt ctgtgcaccg
     2881 tcttcatggg cttcgagggt gaaatcaacg ggcataaagg tggtcgggcg atgatgttgc
     2941 ggccagttaa actcaatccc accggaaatc cacggcccca gcagccccac cagcgcaggt
     3001 ttaatgactt cattgtgata aacaaaatcg cgctgtttca ctttatccca cgcgcgatgc
     3061 acccgaccgc ccagttccgg caggatcatc actttgatgt agtcgttttc cagccacacc
     3121 gcctgccagg atttcagggt tttctgctcg ctcagcgtat cggtcacgcc gtagggataa
     3181 accgcgccgg acgatccctg ataaacgcga ttttccagga acatgggatg tatatcctgc
     3241 ggcccggttt cataggtcgg gatctcaacg cgctcttgcc acacttttac tggagtcatg
     3301 gtgccctcaa tattaacaag acatactgaa ttaaaagatt tgtggcagtg tattgaacaa
     3361 tctggcaatg ttttcgcgga ataatcacgc aattaactaa acaaggttta gtgaagatga
     3421 gagcctgcat taacaatcaa cagattcgcc accataacaa atgcgtgatt ctggaactgc
     3481 tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg gctggcgcaa atttcgattc
     3541 cggcagtcag taatattttg caggaactgg aaagcgaaaa acgggtggtg aatattgacg
     3601 atgaaagcca gacgcgcggg catagtagcg gtacatggct gattgcgccg gaaggtgact
     3661 ggacgctgtg cctgaacgtg acgcccacca gtattgagtg tcaggttgct aatgcttgtt
     3721 taagtccgaa aggtgaattt gagtatttac agattgatgc accgacgccg caggcgctgc
     3781 tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt gtggccggac cgcaccatca
     3841 atctggcgct ggcaatccac ggtcaggttg atccggtgac cggcgtgtcg caaaccatgc
     3901 cgcaagcgcc gtggacaacg ccggttgagg tgaagtatct gctggaagag aagctgggca
     3961 ttcgggtgat ggtcgataat gactgcgtga tgctggcgct ggcggagaaa tggcaaaata
     4021 attcgcagga acgggatttc tgcgtgatca acgttgatta cggcattggc tcgtcgttcg
     4081 tgattaacga gcaaatttat cgcggcagtt tgtatggtag cggacaaatt ggtcacacca
     4141 tcgttaatcc ggatggcgtc gtctgcgact gtggacgtta cggctgccag gaaaccgtcg
     4201 cctcgttaag tgcattaaaa aaacaggcgc gggtatggct aaaatcacaa ccggttaata
     4261 ctcagcttga tcctgaaaaa ctgacaaccg cgcagttaat cgctgcctgg cagagtggag
     4321 aaccgtggat caccagctgg gttgatcgct ctgccaatgc cattggtttg agtctgtata
     4381 acttcctcaa catcctcaat attaatcaga tttggttgta cggtcgcagt tgtgcctttg
     4441 gtgagaactg gcttaatact attattcgcc agacaggatt taacccgttc gaccgcgacg
     4501 aaggaccgag cgtgaaagcg acgcaaattg gcttcgggca attaagccgc gcacaacagg
     4561 tgctgggaat tggctatttg tatgttgagg cgcagttacg acagatttga tggcgcgata
     4621 acgtagaaag gcttcccgaa ggaagccttt tttcaacaga ttaaccgttt cgcttatgcg
     4681 taaaccgggt aacgtgcgca gatgtcgaga actttacctt tgatgcgctc gataacggct
     4741 tcatcattga tgctgtccag cacgtcacac atccagccag ccagttcttt cgcttctgct
     4801 tctttgaagc cgcgacgcgt aattgccgga gtacccacgc gaataccgga ggtcacaaac
     4861 gggctcttcg gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc acggcccaga
     4921 gcggcgtctg cttctttacc ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg
     4981 ttatcagtgc cgccggaaac cactttgtag ccgcgctcga ggaacacttc taccatcgct
     5041 ttagcgtttt tcgcgacctg ctgctggtaa gttttgaact caggctccat cgcttctttc
     5101 agagcaaccg ctttaccggc gattacgtgc atcaacggac cgccctgacc accagggaaa
     5161 acggcagagt tcagtttttt gtacagctct tcgctaccac ctttcgccag gatcaggccg
     5221 ccgcgcggac ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc
     5281 gggttcgggt agacgccagc agcaaccagg cccgcaacgt gcgccatatc aacgaacagg
     5341 taagcaccga tgctgtcagc gatttcacgc attttcgccc agtccaccac gccggaatat
     5401 gcagagaaac caccgataat cattttcggc ttgtgttctt tggcttgttt ttccagatcg
     5461 gcgtagtcga tatgaccggt agcatcgata ccgtaaggaa cgatgttgta cagtttaccg
     5521 gagaagttaa ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt catacccaga
     5581 acggtatcac ctggttccag cagcgcggtg tagaccgcaa agttagcctg ggagccggag
     5641 tgcggctgga cgttagcgta gtcagcgccg aacagttctt tcgcacgatc gatcgccagt
     5701 tgttcaacga tatcaacata ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca
     5761 gcatatttgt tggtcagctg agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt
     5821 tcggaggcga tcagttcgat gtgctcttcc tgacgtactt tttcctgctc catagcctgc
     5881 cacagttcgg catcataatc ggcaatgttc atttcacgct ttaacatccg catctcctga
     5941 ctcagctaac aataaaattt ttggccttta taggcggtcc tgttggacaa cggcgaacag
     6001 tataaccgaa tcattgtgcg ataacaggtc ttgacaaagg aatttacgca aacgattacc
     6061 ttcaggctac gcaagacttt ggagaataaa gagcttgcaa ccggaaacgg atttcttttc
     6121 aggtttgtga tgcaaatttt tcacttcatc acattctttc tgaaaaacac caaagaacca
     6181 tttacattgc agggctattt tttataagat gcatttgaga tacatcaatt aagatgcaaa
     6241 aaaaggaaga ccatatgctt gacgctcaaa ccatcgctac agtaaaagcc accatccctt
     6301 tactggtgga aacggggcca aagttaaccg cccatttcta cgaccgtatg tttactcata
     6361 acccagaact caaagaaatt tttaacatga gtaaccagcg taatggcgat caacgtgaag
     6421 ccctgtttaa cgctattgcc gcctacgcca gtaatattga aaacctgcct gcgctgctgc
     6481 cagcggtaga aagaatcgcg cagaagcaca ccagcttcca gatcaaaccg gaacagtaca
     6541 acatcgtcgg tgaacacctg ttggcaacgc tggacgaaat gttcagcccg gggcaggaag
     6601 tgctggacgc gtggggtaaa gcctatggtg tactggctaa tgtatttatc aatcgcgagg
     6661 cggaaatcta taacgaaaac gccagcaaag ccggtggttg ggaaggtact cgcgatttcc
     6721 gcattgtggc taaaacaccg cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg
     6781 acggtggcgc agtggcagaa taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg
     6841 aaggtttccc gcatcaggaa attcgtcagt actctttgac tcgcaaaccg gatggcaaag
     6901 gctatcgtat tgcggtgaaa cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc
     6961 acgccaatgt tggcgatgtc gtgaaactgg tcgctccggc aggtgatttc tttatggctg
     7021 tcgcagatga cacaccagtg acgttaatct cttccggtgt tggtcaaacg ccaatgctgg
     7081 caatgctcga cacgctggca aaagcaggcc acacagcaca agtgaactgg ttccatgcgg
     7141 cagaaaatgg cgatgttcac gcctttgccg atgaagttaa ggaactgggg cagtcactgc
     7201 cgcgctttac cgcgcacacc tggtatcgtc agccgagcga agccgatcgc gctaaaggtc
     7261 agtttgatag cgaaggtctg atggatttga gcaaactgga aggtgcgttc agcgatccga
     7321 caatgcagtt ctatctctgc ggcccggttg gcttcatgca gtttaccgcg aaacagttag
     7381 tggatctggg cgtgaagcag gaaaacattc attacgaatg ctttggcccg cataaggtgc
     7441 tgtaatttga tgttgccgga tggaaacatc cggcaaccct tgacgcggtt taaattgccg
     7501 cgtcgtcctc ttcaccggta cggatgcgaa tgacccgtgc cacgtcaaag acgaagattt
     7561 taccgtcacc gattttgccg gtttgcgccg tgcgaataat ggtatcgaca caggtatcga
     7621 caatgtcgtc cggtacgaca atctcaattt tcactttcgg cagaaaatcc accatatact
     7681 ccgcgccgcg gtacagctcg gtatggcctt tctggcgacc aaagcctttc acttcggtca
     7741 ccgtcatgcc ggtaataccg acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg
     7801 gttttataat cgcatcaatc tttttcatgc tattccttga aaaggtcgcc tgtcttttga
     7861 tctgctaaac gtaacacata acgccaattc attccttgaa atcgtttgca tccagctcgt
     7921 gtcgggaaag cagtttataa aattctgtcc ggttgcgccc cgccattctc gccgcgtggg
     7981 tgacgttgcc tttggtaatt tgcagcagct tacgcaaata gttgagttca aactgattac
     8041 gtgcctcaac aaaggttggc agcgccgtgt tttcaccctc cagcgcctgc tctactaatg
     8101 catcgctaat aaccggagat gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact
     8161 ggcgcacatt acccggccag ctagcggtca tcaggcgttt catcgcatca gtggagaacg
     8221 cgcggacaaa cggtttatgt cgctctgccg cctggcgcaa caggtgattt gccagaagcg
     8281 gaatgtcttc tgtgcgctct gccagagccg gaattttcag gctgacaacg ttgaggcggt
     8341 aatagagatc ttcgcggaat tccccgcgcg ccatcgcttt tggcagatca cggtgagtgg
     8401 cagaaataat ccgcacattg atatcaatat cgcggttact gcccagcggg cgcactttac
     8461 gctcctgcaa cacgcgcagc agtttgacct gtaacggcgc aggcatatcg ccaatttcat
     8521 cgagaaatag cgttccgcct tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc
     8581 cagtaaacgc gccacgcgca tgaccaaaca gctccgactc cagcaattgc tcgggcaatg
     8641 cgccacagtt aatagcaata aatggtttgc tgttgcgcgg gctggcgttg tggatagcct
     8701 gggcgaaaat ctctttcccg gtgccgctct gaccgttaat caaaacgctg acgtctgatt
     8761 gcgccaccag ccgcgcctgt tccagcaaac gcagcatcag cgggctgcgg gtgacaattg
     8821 cctcgcgcca gcgttcatcg gtggctggcg cggattgctc cagcgcatcg tcaattgcct
     8881 gatatagcgc gtctttgtcg acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg
     8941 cagcaacggc atcggggata gaaccatgcg cagtaagaat aattactggc attcccggct
     9001 gcactttctg gatttcagca aatagctgca taccgtccat ttcatccatc cgcaggtcgc
     9061 tgataactaa atctactttt tcgcgattca gtacccgtaa tccttcagcg ccactttccg
     9121 ccgtgaccac actgtagcct tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg
     9181 gatcgtcatc gaccaataat aaatgcgcag gtttatggct catcaggagt gacctcatgg
     9241 gtggatggcg cgggcttttc actctcgtgc ggcgtatccg gcgagaaatt tccagccggt
     9301 ttgcgggtcg agagctggcg ttcaatatcg gtcaggtttt ccagcttgcg ggtggtgagt
     9361 tccagttgct gctgtagaac gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg
     9421 gactgctgga gtttactgta acgttggcgc tcttccgcca gttgcagttg cagcgcctga
     9481 ccgtcgcgcc agagttgata cagtgggcga acctgtgccg ggatctcggt acttaacgct
     9541 tcaatacgcg cgaccagctg gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa
     9601 atcccttgtt taaaggtatt ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc
     9661 gactgcgcag gcattaaacg atcagcacaa tccatcgccc gcagccagta aagcggattg
     9721 gtttcggttg atttaccttg cagcgcccag atgtcgctac attcagtaga aagatagtca
     9781 gccagttgat aaaccggaat tttttcttct gctggcgtat caatggctgg cttattgtga
     9841 ttctgcacgc aacccagcaa tgccagacat ggcagccctg ccagccacag ccgtcggagc
     9901 aataatcgtt gaaaaatgtg tcgcatattc accagactta aagcctatcc cagtgggcgt
     9961 aattgttgca gacagtctgg acatggcagc gcggagaaac cggagcgtac atatcgtacg
    10021 tgaagatttc gagcactgcc cggggccgaa ctgacaaata aaatagcctg ataggatagg
    10081 ctgttagcat tatttcgtgt ttttcgacga cggtaattca atgcggaaac aaacgtcttg
    10141 cccgctctcg tcgaccagat acagttcccc ttgcatacgg cgaatacaat ccctggcaat
    10201 gcttaatccc agaccgctgc ccttcaccgc cccttttcgc tggtggcttc cctgaaaaaa
    10261 gggttcgaag atcatggcgc gttcctcttg cggaatgggc gtgcctgtat tgatgacatc
    10321 aatataaacc cgcgcaccat gtgaactgct gcgaaggcaa atgttaccgg attcagcccc
    10381 gtagtgcacc gcattggagt aaagattatc cagtacgctc atcagcagca ttggctccgc
    10441 caggcaagct gttgctttga gatcgacgtc ggtatgcatc attttagccc gtgcgggcag
    10501 gctatgagca gaaaccactg tctccaccag cggtgctaat tcaacattct ccagttccac
    10561 cgcactgtcc gcctgtttac ggttgtaatc aagtagttgt tcgatcagtt tttgcaaatt
    10621 gcggctgctg ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa gcggcccgac
    10681 aacctggtca gccagtaatt cagtgccctc gcgcatactc gccagtggcg tttttaattc
    10741 atgagataaa tgtcttaaaa attgatggcg ttgggattcc agccatgaca ggcgctcact
    10801 taaccaaaga atacgttgcc caaccgagcg taactcgctc ggtccactga acgaaacgct
    10861 attgcccaga gaacgccctt cccccagacg gttgatcatg cgctcgatat ttttcaccgg
    10921 cccgataatc atccgcgtga aaagtagcac catcaccaga ctcaccagaa atagcaccag
    10981 cgattgccaa ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt gctgcccacg
    11041 agagaacacc actgtgcgcg tggcctgcac catttcggta ttggcactgg caaaggcttc
    11101 aagacgcgcg gcggcggcag catcgggacc actgttgtta cactgaagtt gggccagatt
    11161 gttcaaatcc tgacgcaatg cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc
    11221 atcgagcatt tcgctgtaac gcttgcgctg gctttgataa accttcgcaa gcgtagggtc
    11281 gtccagcacg caatactgac ggtagctacg ctccatctcc agcgccgcgt tggtcatcgc
    11341 ttcactacgc cgggcatcaa taagcgtagt gcggttaacc agcgccgcct gatcgctaag
    11401 cgcattcagg ctttgccagg cttgccatgc cagcaccaac aggggtagta gaattagcaa
    11461 aaatgccagc attaccagtt gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt
    11521 gttactctcg tcagacgcga atagcgtgat gctaaccgag gggaagttca gatacaacaa
    11581 agccgggaat tacccggctt tgttatggaa taaggcggtg cctaactcga cgtttcgccc
    11641 gatggttgat atagctacgc tgatatcaga agttggacgg caggcacctt gttgtgcgtc
    11701 attcgaattt tatgtagcac gtcccgaagg ggctgacata agtcggtgaa tgagccactg
    11761 cttactatta tgcagtaact gtgccaataa agaaaatagt ttggtaacgt actgattata
    11821 cgttgctttg agggatttat ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg
    11881 ctaaaaagag acaacttaag ataaacttat tagattgcaa taaaatcaat cacttaagtg
    11941 tcgccagaaa gcgacacggt aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc
    12001 ccggggttgg gagcttatga tagtggttgg tgcttaatgc cgcatccggg ctgcaaaacc
    12061 aatgaactga tgaattaccc caactgctta cgcgcattgc ggaaaatgcg catccatggg
    12121 ccatcctcgc cccagttttc cggatgccag gagttgctga cagtacggaa aacacgttcc
    12181 gggtgcggca tcataatggt gactcgacca ctttcagtcg tgactgccgt aataccgttc
    12241 ggtgaaccgt tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt atcgacatag
    12301 cgcagtgcca ccagcccttt gctttccagt gccgccagat gcgccgcatc acgcacttcc
    12361 acgcgccctt caccatgaga gacagcaatc ggcatttgcg agcccaccat cccctgcaac
    12421 agcagagacg ggctttgggt tacttcaacc aggctgaaac gcgcttcaaa gcgatcggag
    12481 gtattgcgca caaaacgtgg ccacaactca ctacctggga tcagttcgcg caggttagac
    12541 atcatctggc aaccgttaca tacccccagc gccagcgttt gcggacggtg gaagaaggtt
    12601 gcaaactcat cgcgtacacg gtcattgaac aggattgact tcgcccaacc ttcaccggca
    12661 cccagcacat caccgtagga gaaaccaccg cacgcgacca gggcgtggaa atcttccagg
    12721 cccgtgcgtc cggtcagcag gtcactcata tgcacgtcga tagcatcaaa gcctgcacgg
    12781 tggaaagctg ccgccatttc aacatgcgag ttcacgccct gctcacgcag tacggcaact
    12841 ttcggacgtg cgccagtggc aatatacggt gctgccacat cttcgttgat atcgaacgac
    12901 agttttacat tcaggcccgg atcggcgtcg ttagatttcg cctgatgctc ctgatcggca
    12961 cactccgggt tgtcacgcag gcgctgcatc tgccaggtag tttctgccca ccagacacgc
    13021 aacgtggtgc ggctttcgct gaatacagtc tgcccgttgg cggtaatcac aaaacggtca
    13081 ccggaaaccg cctgccctac ataatggaca caatcagcaa gcccatgctg tgccagtacg
    13141 gactcgaccg cttcacggtc agcggcacga acctgaatca ccgcacccag ttcttcgtta
    13201 aacaacgccg ccaggcgatc gtcgcccaga gaggcaatat ccgcgttaat gccgcaatga
    13261 ccagcaaagg ccatttccgc cagcgttacc agcaggccgc catcagagcg gtcgtggtat
    13321 gcgagcagct tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc tttcagttgc
    13381 gcgacgtcgc gtacatctgc cggtttgtcg ccaagctgac gataaacctg cgccagcgcc
    13441 gttgcgccca gcgcgttatt gcctttgccc aaatcaatca gcaatagtgc gttatcttcg
    13501 gtagaaagct gtggcgtgat ggtgtgacgc acatcttcca cgcgggcaaa tgcagaaatc
    13561 accagcgaca gcggcgacgt catttcgcgc tcttcgttac cttcctgcca gcgggttttc
    13621 atcgacatag agtctttacc caccgggatc gtcaggccca gcgccggaca aagctcttcg
    13681 cccacggctt taacggcttc atacaggccc gcatcttcac cagggtggcc tgccgccgcc
    13741 atccagttgg cggaaagttt aatgcgcttg atatcgccaa tctgtgttgc ggcaatattg
    13801 gttaacgctt caccgaccgc cagacgggca gaggcggcga aatccagcag cgcaaccggc
    13861 gcacgctcgc caatcgccat cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc
    13921 gcgcagttag cgaccggcac ctgccacgga cccaccatct gatcgcgcgc taccatgcca
    13981 gttacgctgc ggtcaccgat ggtcaccagg aaagttttct ccgccacggt cggcaaatgc
    14041 agtatgcgtt tcaccgcgtc tgcaatggtg atcccttcac ggaccagcgc atcgcccttc
    14101 gctttcagcg tttgtacatc gcgggtcatc ttcggcgttt taccaagcag gacgtccagc
    14161 ggcagatcga tcggctgatt atcaaaatga cgatcgtgta gagaaagatg cagttcttcg
    14221 gtcgcttcac caatcaccgc gtagggtgcg cgctcacgct tacacagttc gtcaaacagc
    14281 ggtaattgat cggcagcaac cgcgagcacg tagcgttcct gggattcgtt acaccagatt
    14341 tccagcgggc tcatgcctgg ctcgtcgctc agaatgtcgc gcagttcaaa tttaccgcca
    14401 cgcccgccgt cgctcaccag ttccggcata gcgttagaaa gaccgcctgc gccaacgtcg
    14461 tggataaaca gtattgggtt ggcatcacca agctgccagc aacggtcaat cacttcctgg
    14521 cagcgacgct ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca
    14581 gactgaccag acgccatgga agacgccgcg ccgccgccaa gaccgatgtt cattgccggg
    14641 ccgccgagaa cgaccagctt cgcgccaacg ttgatctcgc ctttttgtac gtgatcggcg
    14701 cgaatgttgc cgatcccgcc cgccagcatg atcggtttgt gataaccgcg cagctcttcg
    14761 ccgttgtggc tgttcacttt ttcttcataa gtacggaagt agccgttcag tgccggacga
    14821 ccaaattcgt tgttaaacgc cgcaccgccc agcgggcctt cggtcatgat gtccagcgcg
    14881 gtgacaatgc gctcaggctt accgaaatct tcttcccatg gctgttcgaa gccaagaatt
    14941 cgcaggttgg aaacggagaa accaaccaaa cccgctttcg gcttcgcgcc gcgcccggtg
    15001 gcaccttcat cgcggatttc accgccggaa ccggtcgccg cgcccggcca cggagaaatc
    15061 gccgtcgggt ggttgtgagt ttcgactttc atcagaatat gcgccggttc ctgatggaaa
    15121 tcatagcggc ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc ttccattacg
    15181 gcggcgttat ctttataagc agagagaacg tgatctggcg tggtttcgaa agtatttttg
    15241 atcatcctga acagcgattt cggctgttgt tcaccgtcga taatccagtc ggcgttaaaa
    15301 attttgtggc ggcagtgctc agagttcgcc tgagcgaaca tatacagctc gatgtcgttc
    15361 gggttacgac caagctttgt gaaagcatcc tgcagatagt caatttcatc ttccgccaga
    15421 gccaggccaa gacgcaggtt agcgtcgatc agcgcctgac ggccctgccc cagcaaatca
    15481 acgcttgtga ccggagtcgg ttgatggtgg gcaaacaact gctctgcatc atctaaatca
    15541 aaaaagacag tttccatcat gcggtcgtgc agttcagcgg taacctgctg ccattgttca
    15601 ttggtcagcg taccggcttc tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt
    15661 tgtagcccgc agttatgggc aatatcggtc gctttcgaag accagggaga gatggtgcca
    15721 ggacgcgggg tcaccagcag gagtttgcct tgcggggcgt ggctggcgag tgccggacca
    15781 tatttcagca ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca
    15841 gcaaaatgga catactcggc gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt
    15901 gccagcagtt tgttgattcg gaatgccgac agtgcaggcg aaccacgcag aatttccatc
    15961 ataagtctct cgtcttctaa gctttcggtg tacccaaggg ggggaaacgg gcgtcattat
    16021 aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat aaaatcacta tcgtgaatta
    16081 gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg
    16141 ccgctcattt atgagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac
    16201 gacacagaga attaactaat tgaaaaaatt aaagattaat tatctgttca tcggcattct
    16261 ggcactgctg ctcgcggtcg ctctctggcc atccattccc tggtttggta aagccgacaa
    16321 ccgtatcgcc gccattcaag cgcggggagt gttgcgtgtg agcaccattc atactcccct
    16381 gacttataac gaaatcaacg ggaaaccttt tggcctggat tacgaactgg cgaaacagtt
    16441 tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca gccagctgtt
    16501 tgacgacctt gataatggta acgccgacct gctggcggca ggactggtct ataacagtga
    16561 gcgggtaaaa aattatcagc ctggccctac ctattattcc gtgtcacaac aactggttta
    16621 taaagtgggt cagtatcgcc cacgtacgct gtgcaacctg acggcggagc aactcaccgt
    16681 tgcaccgggt catgtggtgg ttaacgatct ccagacccta aaagaaacaa aattcccgga
    16741 attaagctgg aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga
    16801 aggaaaactc gattacacca ttgctgattc tgtcgccatc agcctgtttc agcgcgttca
    16861 cccggagctc gccgtagcgc tcgatatcac cgatgaacaa ccggtgactt ggtttagccc
    16921 gttagatggc gataataccc tttccgccgc cctgctcgac ttcttcaacg aaatgaatga
    16981 agacggtacg ctggcacgca ttgaagagaa atacctgggg catggcgatg attttgatta
    17041 cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt taaagcccct
    17101 gtttgagaaa tacgccgaag atattgactg gcgtttgctg gccgctattg cttatcagga
    17161 atcgcactgg gatgcacagg ccacttcacc gacgggtgtg cgcggcatga tgatgttaac
    17221 caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac agagcatcag
    17281 cggtggcgtg cgttatttgc aggatatgat gagtaaagtg ccggaaagtg tgccggagaa
    17341 cgagcggatc tggtttgccc tcgctgcgta caatatgggc tatgcgcata tgctggatgc
    17401 ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg taaaacagcg
    17461 tctgccttta cttagccaga aaccctatta cagcaagctg acttacggct acgctcgtgg
    17521 acatgaagcc tacgcttatg tcgaaaatat tcgtaagtat caaattagcc tggtgggtta
    17581 tctgcaagag aaagagaagc aggctacaga agcggcgatg caactggcgc aggattatcc
    17641 ggcggtatcg cctacggagt tgggcaaaga gaaatttcct tttctctcgt ttctttccca
    17701 gtcgtcatca aactatttga cccattctcc ctctctgctg ttttccagga aagggagtga
    17761 agagaaacaa aattaatccg tcgaggattg cgcttttttc tgcgctttaa tttcctggcg
    17821 gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca tccgccagta ttccttccgt
    17881 aatttccact cggtgattca tacccggatg atgcagcaca tccattaaag atcccgcagc
    17941 gccagttttc gcgtcacgcg caccaaagac cacgcgacca atgcgactgt ggatcatcgc
    18001 tccggcacac attacacatg gttcaagcgt gacatacaac gtggcgtcga tcagacgata
    18061 attttgcatc accagaccac cctgccgcag ggccatgatt tctgcatgtg cggtgggatc
    18121 atggcgacca atcgggcggt tccagccttc gccgattacc cgattgttat gcactaatac
    18181 cgcgccgacc ggcacttccc gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg
    18241 catccagtat tcgtggctaa attcgacttc agacaaaaag aaaactccgg ttataaaagc
    18301 gcggcgcatt atacacggac gctatgcttt actctattcc agttgctgga gttcaccgcg
    18361 cggggttact cgccaacgat gctgacagaa ataaagcagc ggattgtcct gattactgtc
    18421 gctatagcca ctgtacagcc gcagcggagt gccgattttg cgctccagtt gtgcgacctt
    18481 ttcatgtccc agacaacgca tcgtcaatac ccaaccacca tagccacgct gaatttggct
    18541 ggcgataaga ttaacccgcg gcagccaggg cgtatcgaaa taaaccgctt caaccagcgg
    18601 ctgcggagag ccggtaatca accagatatc agcatcggaa cttaacaggt aggtggttaa
    18661 tcgctcctga accagcggaa aggcggtaac attgtcgcga aaccagcgca cgaaatcggc
    18721 ctgcaacgtc tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca gaagcagact
    18781 catcggccag cgtgccgcac gaccttttat caataacgca atggctataa tcggtaacaa
    18841 cggcaggaca agtaacgcat tcagcggttg gcgacgtagt aaatagcgca gaaaactgcc
    18901 gaacatatcc tgctgatgca atgttccatc taagtcaaaa aacaccacac gacgctcgtg
    18961 agttgccaaa ccatactcct ctggactgaa acatcctgat taattttcgc tactacatag
    19021 cctaacagat agatcatcac ttttccggca acaatcgacc gcatcatggc taactggaat
    19081 ttttaattca tggcaattag cggcaatgga atataaaatt cactcgcgtg tgcctcatat
    19141 tgcctgttgt cgccactatt tcatcgcgag gaaagatatg aacggcttac ttcgtatccg
    19201 tcagcgttac caggggcttg cccagagtga taaaaaactg gcggattatc tgctgctaca
    19261 acctgatacg gcgcgccatt taagctctca gcaactggcc aacgaagccg gagtcagtca
    19321 gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa ggttttccgg cgcttaagtt
    19381 ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc tccgtgccca ttcataacca
    19441 aatccgcggt gatgatccgt tacggctggt cggcgaaaaa ctgattaaag aaaatactgc
    19501 cgctatgtac gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat
    19561 gttgcgctct gcgcggcgga taattctgac cggtattggc gcttcgggtc tggtggcgca
    19621 aaactttgcc tggaagctga tgaagattgg cttcaatgct gccgcagtgc gcgatatgca
    19681 tgcgctactc gcaacagtac aggcgtcgtc ccctgacgat ctgttattag ccatttccta
    19741 caccggtgta cgacgcgagt taaacctggc ggcagatgag atgctgcgag tgggcggaaa
    19801 agtgctggcg attaccggct ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct
    19861 gtataccatt gccgaagaac aagcgacaaa cagtgcttca atctctgctt gtcacgctca
    19921 gggaatgtta acggatttgc tgttcattgc gctgattcag caggatctgg aactggcacc
    19981 agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa ataatgcgcg
    20041 tataatgccc gcccggtttg tgttgttttg agagtttcct tatggcgttg ttaatcacta
    20101 aaaaatgcat caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg atttcaatgg
    20161 gagatcatat ctacgagatt aacagcgata agtgtaccga atgcgtaggg cactacgaga
    20221 caccaacctg ccagaaggtg tgcccgatcc ccaatactat tgtgaaagat ccggcgcatg
    20281 tcgagacaga agaacagttg tgggataaat ttgtgctgat gcaccacgcg gataaaattt
    20341 aatattctac tctggaagta gagtattaat tatattactg ggaagcctta acgccattat
    20401 atttatttaa ttgatgacat tagcataatc attcactaag ttaatttata tagtatctgc
    20461 caagacactt atttatagtt attaaaggcg cgtccgattg gttcaccgga cgcaccttaa
    20521 gtacgtttcc ttgtgttata agaacagaag gatcagctgt aaaacagcaa tgatgatttt
    20581 gatgacccgt ttaatcaggt atcggcaatc agtcattcgt ttttccttaa acaaggaatg
    20641 cagccgttca gattagccct tacatctccc caaaactgaa cgtgcgagtt attgagggtg
    20701 catgctgcac tccacaccag agctttgacg acaccactcg tttcaatggg ggaattctgt
    20761 ggcatggtgt aaagcacagc aaaatcttca ataacgaagc caattttaat gtacttacga
    20821 attggcggtc acgttaatct ttccatcaat attgctttct tcgtaaaggc tcgagttttt
    20881 atgctaaaga ttgcaaattg cttgtaaaag ataagtacac tgatccataa tcgctgttgt
    20941 tgagggtgca tgctgcacaa aattaaagtt aaaaagtaaa acccccgttc cttaccagtt
    21001 cgggggtttt actttttaaa gagaacggta ttattttaac tttcaataat taccgtggca
    21061 caagcatagt ggcgttcatc tgccagcgtt acatgcatat ttacaacgcc gagcttttcc
    21121 gccagtttta atgcctcacc ccatagccgt agccgtggtt tgccgagctc atcattgaat
    21181 acttcaaatt gattaaacgc cagaccattg cggatcccgg tgccaaacgc ttttgctgcg
    21241 gcttctttca cagcaaaacg cttcgccaga aaacgcaccg gctggtggtg cgttttccag
    21301 attgcccatt cgttatcgct taatacgcgg cgtgccaggc gctcaccgga tcgggcgatc
    21361 actgcttcta tgcgagcgat ctctacgata tccatgccta aacctaatat tgccattagc
    21421 cacgcgcttc cagcatcaga cgcttcattt ctgccaccgc atctttcagt ccggtcatca
    21481 ctgcacggcc aataatggca tgaccgatat tcagttcatg catctcaggg atggcggcaa
    21541 tggctttcac gttgtgatag gtcagaccgt gtccggcgtt aactttcaga ccgagactag
    21601 cggcaaaggt cgcggctttg gcgatacgca ccagctcttg cgcctgttcg gcgtcagttt
    21661 tggcatcagc atagcaaccg gtgtggatct cgataaacgg tgcgccaacc tctgccgcag
    21721 ctttgatctg ctcttcatcg gcgtcgataa agagagaaac ctgaatcccg gcatctgtca
    21781 gacgtttgca ggcatcgcgc attttttcac gctgccctgc aacgtccagg ccgccttcgg
    21841 ttgttacttc ctgacgcttt tccggtacca ggcagcaaaa atgtggcttc gtctcaacgg
    21901 cgatcgccag catctcttcg gtcactgcca tctccagatt cattcgggta tccagcgtct
    21961 gccgcaggat gcgcacatcg cggtcagtaa tgtggcggcg atcttcacgt aaatgcactg
    22021 taatgccgtc cgctcccgcc tgctcggcaa taaacgccgc ctgcaccgga tccgggtaag
    22081 cagtaccgcg tgcgttacgc agcgttgcaa tatggtcaat gttgacgcct aacagtaatt
    22141 cagccatgac aatcctcatc attcataatg tgttttcacc gttcgcttag gcataaactg
    22201 ccggaacagt tccctgctct ttaatggttt accgccaaga tacggcttaa gcgccatgcg
    22261 ggtaaagcgt ttcgcggcgc gtagtgtgtc tgcgtcagga aattcccgcg cgtttaacgc
    22321 ttttaactgc cttccggtga acgttttatt gtcgataacg acgcttgcga taaacccttt
    22381 ttcttcgcga taacgatacg tcatggtgtc atctaccggc tcgccgctac ccgcgcaata
    22441 ggtaaaattg acgccatagc ccagatgccc gagcagtgcc agttcaaagc ggcgcagagc
    22501 gggttctggc gtaccagtgg cacctgcaag agactgaatg cagtgcaaat aatcgaaaaa
    22561 gagttcagag aagcgcgtct cgtattccag tacgcgggag agaagttcgt tgatgtacag
    22621 accgctgtaa agcgtgatac tgcttaatgg cagcgccagc gagacagctt cagcactgcg
    22681 cagcgttttg acttcgccac gcccgccgaa acgtagcaag agaggggtga aaggctgtaa
    22741 tgcacctttc agagtagagc gtttagagcg tgcgcctttg gcaaccagac gcacgcgacc
    22801 cgattcctcc gtgaagacgt ccagcatcag gctggtttcg ctccacgggc gactatgcag
    22861 gacaaatgcg cgctgccagc cttccatcgg agtgactctt aaagatcgtc aacgtaaccg
    22921 agactgcgca gtgcgcgttc gtcgtcggcc caaccggatt tcacttttac ccacagttca
    22981 aggtggacag gcgcttcgaa catttcctgc atgtctttac gcgcttcaat cccgatggtt
    23041 ttgatcttgg cccctttgtt gccaataacc atcttcttct gcccttcacg ctcaacgaga
    23101 atcaaaccat tgatgtcata accaccgcgt tcgttagaga cgaaacgttc gatctccacg
    23161 gtcacggagt acggcagttc agcgccgagg aaacgcatca gtttttcgcg gatgatttca
    23221 gacgccataa aacgctgtga gcgatcggtg atgtaatctt ccgggaagtg atgggtcgct
    23281 tcaggcagat gcttacgcac gattgctgcg atagtgtcga cattcagccc ggtttcagca
    23341 gagatcggca cgatatcgag gaagttcatc tgacttgcca ggaactgtaa gtgcggcagc
    23401 agatcggctt tttcctgcac gttatccact ttgttcaccg cgaggatcac cggcgctttg
    23461 ccatcgcgca gtttgttgag caccatttcg tcgtccggcg tccagcgggt gccttcaacg
    23521 acaaaaatca ccagctcaac gtcgccaata gagctgctcg ccgctttgtt catcaggcgg
    23581 ttaatagcgc gtttttcttc catatgcagg ccaggggtat caacgtagat cgcctgatac
    23641 gcgccttcag tatggatccc cacaatgcgg tgacgagttg tttgcgcttt gcgggaggtg
    23701 atggagatct tctgccccag cagtttgttc aacaatgtgg atttgccaac gttcggacgt
    23761 ccgacgatgg caataaatcc gcagtaactt ttatcgatgc tcattccagc tccagttttt
    23821 tcaacgcctg ttcggcggca gcctgctcag ccttacgacg gcttgaacct gtgccaacca
    23881 ccggttcact caggccgctg acctggcagt ggatagtaaa ttcctgatcg tgcgcttcgc
    23941 cacgtacctg gactaccaga taagtcggca gcggcagatg gcgaccctgc aaatattctt
    24001 gcaagcgcgt tttcggatct ttttgtttat cgcctgggct aatttcgtcc aaacgagttt
    24061 gataccagtt gaggattaat ttctcgacgg tttgaatatc actgtcgagg aatacgccac
    24121 caattaatgc ttcgacggta tcggcgagaa ttgactcacg acgaaatcca ccgcttttaa
    24181 gttcacctgg ccctaaacgt aagcactcgc ctaactcaaa ttcgcgcgcc agttccgcca
    24241 gcgtattgcc acggaccagc gtggcgcgca tccggctcat atcgccttca tccacacaag
    24301 ggaaacggtg ataaagcgca ttggcgataa cgtagctcag aatagagtcg cctaaaaatt
    24361 ctaaacgctc gttatgtttg ctgctggcac tacgatgagt taatgcctgc tgcaacagtt
    24421 cctgatgatt aaaagtgtag cccagcttcc gttgaagccg attaattacg atggggttca
    24481 tgcgatacca ataaataaat gcgtcaacaa ttcagcacac gaaacagacc tgatatacat
    24541 ggctctgcta actgcttcgc tgcagtttca gtttaccggt atatggggac caacgctgtt
    24601 tcgtgtgccg tggcaacctg gaggtgccaa ccttaaactt cgggggaata ttctatacac
    24661 aacgacgggg gatgtcgtta gccacgggag atttatctca taaataattc acgttgtcgc
    24721 cataacggcg acaacgtgaa cgaagatggg ctattaatgg atgccgccaa tgcgacttaa
    24781 gcgcacacct gtcggccatt cgccttcttg cttatcgaaa ctcatccaga tagccgttgc
    24841 ccgaccgacc agattcgctt caggcacaaa accccagtaa cggctgtccg cgctgttgtc
    24901 gcggttgtcg cccatcatga agtattgtcc cggcggaaca atccaggttg ccagttgttg
    24961 ccctggctgc tggtaataca tccccacctg gtcctgcgca atcggcactg tcagaattcg
    25021 gtgcgtcaca tcacccagtg tctctttacg ctcggaaaga cgaattccat tttctttggt
    25081 ttcgtttttc ggcacttcaa agaatccgct ggtcgcttcc ccaccattac ggcgtgagaa
    25141 ggtctgaacg aaatcgctcg gttccacgtt ggagtaggtg accggtagcg cgttttcaca
    25201 cgcctggccg gaactgcatc ccggttgaat cgtcagctct tttgagaccg gatcgtaagt
    25261 gactttatcg cccggtaaac ccaccgcgcg cttgatgtaa tcaagctttg gatcttccgg
    25321 atatttaaag accacgatat cgccgcgttt cggatgaccg gtttcgatca gcgttttctg
    25381 gtagatagga tctttaatgc cataagcaaa cttctctacc agaataaaat caccgattaa
    25441 cagagtcggc atcatcgaac ctgacgggat ctggaacggt tcataaataa acgaacgtac
    25501 aatcaatacg atagccagca ccggaaaaac agaagcaccg gtttccagcc agccaggctt
    25561 cggcgcaacc tttttcaggg ttgctttatc cagtgagtca ccggcagccg cctgcgccgc
    25621 tgcctgacgt tcccgccgtt taggtgcgaa aaagaattta tccacgcacc ataaaatgcc
    25681 cgtcaccagt gtggcaatca ccagaatcag ggcaaacata ttcgccatgc caactcctaa
    25741 gggttatttg ttgtctttgc cgacgtgcag aatggcgagg aacgcttcct gcggcagctc
    25801 gacgttaccg atctgcttca tgcgtttctt accttctttc tgcttctgca gcagcttttt
    25861 cttacggctg atatcgccgc cataacattt agccagtacg tttttacgca gctgtttcac
    25921 ggtggatcgc gcaataatgt gcgtaccaat cgctgcctga atggcgatat caaactgctg
    25981 gcgtgggatc agatctttca tcttctccac caactcgcga ccgcggtttt gcgaattatc
    26041 acggtgggtg atcagcgcca acgcatcaac acgttcaccg ttgattaata cgtctacacg
    26101 taccatgtcg gacgcctgga agcgtttgaa gttgtaatcc agagacgcat aaccacgcga
    26161 ggtagatttc aggcgatcga agaagtcgag caccacttcc gccatcggga tctcgtacgt
    26221 cagcgccacc tgattaccgt ggtaaaccat attggtctgc acgccgcgtt tttctacgca
    26281 cagcgtaata acgttgccga gatatgcctg cggcagcagc atgtgacact ctgcaatcgg
    26341 ctcgcgcagt tcgtaaatgt tatttaccgc aggcagcttg gatgggctgt cgacgtagat
    26401 aacttccctt gacgtggttt caacttcata cactacggtc ggcgcagtgg tgatcagatc
    26461 cagatcgtat tcacgttcca gacgttcctg gatgatctcc atgtgcagca ggccaaggaa
    26521 gccgcagcgg aaaccaaagc ccagcgcgct ggagctttcc ggctcataga acagtgaggc
    26581 atcattcagg ctgagtttac ccagcgcgtc acggaaggct tcatagtcgt cggaacttac
    26641 cgggaacaga ccggcgtata cctgcggttt gactttctta aagccaggca gcgccttttc
    26701 tgccggatta cgcgccagcg ttaaggtatc gccgactgga gcgccgtgga tatctttaat
    26761 cgcacatacc agccagccta cttcgccaca tttcagttca gtgcggtcaa cctgtttcgg
    26821 cgtgaagatg cccagacggt cggcgttata ggtctgcccg gtactcatga ctttcacttt
    26881 gtcgccctta cgcagggtgc cgtttttaat acggataagt gaaacaacgc ccaggtagtt
    26941 gtcgaaccat gagtcgataa ttagtgcctg caacgggcct tccggatcgc cttccggcgg
    27001 cggaatgtcg cgcaccagac gttcgagaac gtcctgcacg ccaacgccgg ttttcgctga
    27061 acagcgcacc gcgtcagtgg cgtcgatgcc gacgatatct tcaatttctt ccgccacgcg
    27121 ttcaggatcg gctgccggca ggtcaatctt gttcagtact ggcaccactt cgagatccat
    27181 ttccatggcg gtgtagcagt ttgccagggt ttgcgcttcc acgccctgcc cggcgtcgac
    27241 caccagcaat gcaccttcac aggcagccag cgaacgggaa acttcatagg agaagtctac
    27301 gtggcccggg gtgtcgataa agttaagctg ataggtttcg ccgtcagacg ctttgtagtc
    27361 cagcgtcacg ctttgcgctt tgatggtaat gccacgctca cgctcaagat ccatggaatc
    27421 gagaacctgc gcctccattt cacggtcaga caggccaccg cagatctgga taatacggtc
    27481 agacagcgtc gatttaccgt ggtcaatgtg agctatgatc gaaaagttac gtatattctt
    27541 cataaagtat gattattgtg ccttaatgcc cggttaacca ggcttttaga agtcgctgtt
    27601 ctgagcttaa cgtctgtatt aatagaaacg ccgcattcta cactacaaca ttgaggcgag
    27661 gaaatgttca taccgtatgg attgtggtat ctggaaacgt cctcgcattt gttatgcaaa
    27721 atgcaacaaa gccagtgaaa tcactggctc gcgtcttccg aagatgtttc aaatcgcaca
    27781 aggccaggcg gcaatgccac gcttaagatg atcggttgcc attctgcccg ggcagcaaac
    27841 ttacgcgagt agccgcgcgc aatcaggaac ccgccaatac cgccgaggac cgcaccacat
    27901 aatgctgcga cgtcagaagc aaagagtagc tgaaatagcg aagcgatgag gaataatccc
    27961 accagcggcg acatataaac cagtaatgcg gagctaagca ggctgccttc ggcgatcccc
    28021 aattccactt tttgccccgg cactaacggt tcatcacagg gtacgacaat ggtatgcgtg
    28081 gtttgcgggc caagtttatt caacacgcgg ctaccgcaac cggcgcgtga agcgcagctg
    28141 ctgcatgaag ctttaacatc acaactgacc agcgcctgcc cgttttgcca ggagacgacg
    28201 gtagcccact ctttgatcat tgcgctgccc cgaacttaat attctcggca atgcgtttcg
    28261 ccgtttgcgg cggcagttca ccgacaatgg tgatttcggc gttatcacgt acgcttgtac
    28321 tgacggttct gcgtccggtg cgcaacatct gatcggtgct cgatggcgta gcgcggttaa
    28381 cgtttaccga gaagctgaat aatccgtcag aatagagacg tgattcgata ggcatgttgt
    28441 ccatcgtcgg tagcggacgt cgactactgg aaacttcgct aaaaccctgt ggcaaccagg
    28501 ttggcgtcca gctgaattta gctttttcac ctacaggaac agaaagcaac ggcggcaaat
    28561 ttgccttcgc cagcgtctgc atactgctgc tgatatcctg attgacgtta aaagcaatca
    28621 cgcgaaattg ttccagcgtt tcaccatcgc gatcaaggag atcaacccgc atcggtaatt
    28681 tcgattcggt gtccatccac acgatgtagc tgtagcgtgt accatctcgg gcaaccacgc
    28741 gaatgacttc gcaaagacga tcagcaatac gcgtgcgccc gacggagata aagtcgtagt
    28801 aaggagaaag gcgtttgaaa tcggtataga tgagcgatgg cagagaatca acaatgtaat
    28861 cgccattaag cgtgaacggt tcaagtcccg gttcaaaata gctgatttca tcgccgcgct
    28921 gtaccacttc ccggcgcggg ccatccattt gcaacaattg tgcaagagga cggttatcga
    28981 ggcgtgcatg tcgataacgc agagactcaa caccctgttt attgatgctg atgaatgaca
    29041 gctcgtaatt cagtgactga ctggccaggt tcatctgctg taataacgcc ccggacgcgg
    29101 gagtggccga ggcgttagca gagaataaca ggctacctgt cactaatgac atggcaaacc
    29161 aaagttgctt cattactgcg attgcgttcc taaagtttga attcctggca cctgtacagc
    29221 ggcttgctgg gtttgcgcct gctcaaactg aagctgttca gagtggagtc ggcgttgcag
    29281 ttcgtaatcc tgcaacattg cattaatgcg acgacgctgc tcctgtacct gctgctgttg
    29341 accattgttt gcggtcgctt cagaaggtac tcccaggctt accgggctgg ctttacccat
    29401 catcggcagt gtattaaata ccggcgtttc gggctgctgg gacgtttcag attgtccatt
    29461 atagtgctgg acgccaacga taactgcaag cgatacgcat gctgctacgc ccatttgggt
    29521 aagctgtgcc gcccacggac gtactttctg ccagaatggc attttctgcc attgatgcgg
    29581 cgcaggctgg gcttccggga tcaatgtcgc cggttgacgt actggctctt cttcaatggc
    29641 ggccatcacg cgtgaagaga tatcgaaatg gagcacctcg ggagtatcac cccgcattga
    29701 gtcacggatt aagtgatagc tttcccaggt tttctgcatt tctgggttat gagccagttc
    29761 gttaagcagc tcactatcca gcgtttcgcc atccattaaa gcggaaagtt gttctttctg
    29821 catgcctaat acccttatcc agtatcccgc tatcgtcaac gcctgataag cggttgaact
    29881 ttgttatcaa tagcttccct cgctcggaag atacgtgaac gcaccgtacc taccggacaa
    29941 tccatgatag cggctatctc ttcatagctc aggccatcca gctcccgcaa ggttattgcc
    30001 atgcgcaaat cttccgggag ggactcaata gttcggaaaa ctatctgtct cagttcttct
    30061 gacaacatta agttctcagg gttcgaaatt tctttcaacg cgccgccact ttcgaagttt
    30121 tcagcttcaa tggcatccac atcactggaa ggtggacgac gcccctgagc aaccaggtaa
    30181 tttttcgctg tatttacagc aatccgatac agccatgtat aaaaagcgct atctccccgg
    30241 aacgaatcca gcgcacgata ggctttaata aaagcttctt gtaccacatc gggaacatca
    30301 cccgacggca catagcggga aaccagactc gccactttat gctgatagcg cactaccagt
    30361 aagttaaagg ctttctgatc tcccttctgg acccgttcaa ccaggacctg gtccgttaac
    30421 tgctcgctca tccgaggtaa agtctcccca aaccaaattt ccacgcgcta tcgaaacgcc
    30481 actccattag ctgcaatttg agcaagcaaa gggttagagt gtctcgtttt tgtaaagttc
    30541 cgtaacgcat ctgtttttgt ttgtcatgct gtagacggat cattatctat cattataagt
    30601 ctacagaatc tgaacatcgc attatctgtg tagaaatgcc catttaactg cctgaagagt
    30661 aacccaacgg cctttttatt tcaccaccta atcctccacc agccagtaac ttctcttttt
    30721 ctcgccgccc tgcatcagcg tgtttagcaa ctgtaacaaa tattaaaata gcaggtgttt
    30781 attcgcacaa catgatgcta tgctgaccaa accgtgttta gtaaattaga caaagaaaat
    30841 gaatactctc cctgaacatt catgtgacgt gttgattatt ggtagcggcg cagccggact
    30901 ttcactggcg ctacgcctgg ctgaccagca tcaggtcatc gttctaagta aaggcccggt
    30961 aacggaaggt tcaacatttt atgcccaggg cggtattgcc gccgtgtttg atgaaactga
    31021 cagcattgac tcgcatgtgg aagacacatt gattgccggg gctggtattt gcgatcgcca
    31081 tgcagttgaa tttgtcgcca gcaatgctcg ttcctgtgtg caatggttaa ttgaccaggg
    31141 ggttttgttt gatacccacg ttcaaccgaa tggcgaagaa agctaccatc tgacccgtga
    31201 aggtggacat agtcaccgtc gtattcttca tgccgctgac gccaccggta gagaagtaca
    31261 atccacgctg gtgagcaagg cgcagaacca tccgaatatt cgtgtgctgg agcgcagcaa
    31321 cgcagttgat ctgattgtct ctgacaaaat tggcctgccg ggcacgcgac
//
LOCUS       DAJTNA010000026        31059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTNA010000026 DAJTNA010000000
VERSION     DAJTNA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31059
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.026"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(106..984)
                     /gene="hslO"
                     /locus_tag="OO161_001033"
     CDS             complement(106..984)
                     /gene="hslO"
                     /locus_tag="OO161_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5462189.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(1009..1410)
                     /gene="hslR"
                     /locus_tag="OO161_001034"
     CDS             complement(1009..1410)
                     /gene="hslR"
                     /locus_tag="OO161_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5462190.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASETALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(1421..2089)
                     /gene="yrfG"
                     /locus_tag="OO161_001035"
     CDS             complement(1421..2089)
                     /gene="yrfG"
                     /locus_tag="OO161_001035"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5462191.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(2154..4289)
                     /gene="igaA"
                     /locus_tag="OO161_001036"
     CDS             complement(2154..4289)
                     /gene="igaA"
                     /locus_tag="OO161_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5462192.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTLVLQVPSPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            4609..5169
                     /gene="nudE"
                     /locus_tag="OO161_001037"
     CDS             4609..5169
                     /gene="nudE"
                     /locus_tag="OO161_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5462193.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(5334..7886)
                     /gene="mrcA"
                     /locus_tag="OO161_001038"
     CDS             complement(5334..7886)
                     /gene="mrcA"
                     /locus_tag="OO161_001038"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5462194.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            8006..8793
                     /gene="hofM"
                     /locus_tag="OO161_001039"
                     /pseudo
     CDS             8006..8793
                     /gene="hofM"
                     /locus_tag="OO161_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
     gene            8793..9290
                     /locus_tag="OO161_001040"
     CDS             8793..9290
                     /locus_tag="OO161_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HCR5462195.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQSQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDAQGRWQFEYQLTRKVS
                     DEHVL"
     gene            9274..9714
                     /gene="hofO"
                     /locus_tag="OO161_001041"
     CDS             9274..9714
                     /gene="hofO"
                     /locus_tag="OO161_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5462196.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            9704..10108
                     /gene="hofP"
                     /locus_tag="OO161_001042"
     CDS             9704..10108
                     /gene="hofP"
                     /locus_tag="OO161_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR5462197.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            10020..11258
                     /gene="hofQ"
                     /locus_tag="OO161_001043"
     CDS             10020..11258
                     /gene="hofQ"
                     /locus_tag="OO161_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5462198.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            11659..12180
                     /gene="aroK"
                     /locus_tag="OO161_001044"
     CDS             11659..12180
                     /gene="aroK"
                     /locus_tag="OO161_001044"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5462199.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            12237..13325
                     /gene="aroB"
                     /locus_tag="OO161_001045"
     CDS             12237..13325
                     /gene="aroB"
                     /locus_tag="OO161_001045"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5462200.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            13417..14703
                     /gene="damX"
                     /locus_tag="OO161_001046"
     CDS             13417..14703
                     /gene="damX"
                     /locus_tag="OO161_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5462201.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAVTDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            14810..15646
                     /gene="dam"
                     /locus_tag="OO161_001047"
     CDS             14810..15646
                     /gene="dam"
                     /locus_tag="OO161_001047"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5462202.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLLGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            15664..16341
                     /gene="rpe"
                     /locus_tag="OO161_001048"
     CDS             15664..16341
                     /gene="rpe"
                     /locus_tag="OO161_001048"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5462203.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            16334..17092
                     /gene="gph"
                     /locus_tag="OO161_001049"
     CDS             16334..17092
                     /gene="gph"
                     /locus_tag="OO161_001049"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5462204.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            17085..18089
                     /gene="trpS"
                     /locus_tag="OO161_001050"
     CDS             17085..18089
                     /gene="trpS"
                     /locus_tag="OO161_001050"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5462205.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            18358..19263
                     /locus_tag="OO161_001051"
     CDS             18358..19263
                     /locus_tag="OO161_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462206.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            19280..19642
                     /locus_tag="OO161_001052"
     CDS             19280..19642
                     /locus_tag="OO161_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5462207.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            19726..20889
                     /locus_tag="OO161_001053"
     CDS             19726..20889
                     /locus_tag="OO161_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5462208.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            20889..22115
                     /locus_tag="OO161_001054"
     CDS             20889..22115
                     /locus_tag="OO161_001054"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5462209.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            22112..22990
                     /locus_tag="OO161_001055"
     CDS             22112..22990
                     /locus_tag="OO161_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5462210.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            23001..23354
                     /locus_tag="OO161_001056"
     CDS             23001..23354
                     /locus_tag="OO161_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5462211.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            23366..24670
                     /locus_tag="OO161_001057"
     CDS             23366..24670
                     /locus_tag="OO161_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5462212.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERMSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            24682..25767
                     /locus_tag="OO161_001058"
     CDS             24682..25767
                     /locus_tag="OO161_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5462213.1"
                     /translation="MKTFTLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(25919..26650)
                     /gene="phoB"
                     /locus_tag="OO161_001059"
     CDS             complement(25919..26650)
                     /gene="phoB"
                     /locus_tag="OO161_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5462214.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(26750..27535)
                     /gene="frlD"
                     /locus_tag="OO161_001060"
     CDS             complement(26750..27535)
                     /gene="frlD"
                     /locus_tag="OO161_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5462215.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLDENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(27532..28362)
                     /gene="frlC"
                     /locus_tag="OO161_001061"
     CDS             complement(27532..28362)
                     /gene="frlC"
                     /locus_tag="OO161_001061"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5462216.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(28412..29269)
                     /gene="frlB"
                     /locus_tag="OO161_001062"
                     /pseudo
     CDS             complement(28412..29269)
                     /gene="frlB"
                     /locus_tag="OO161_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
     gene            complement(29261..30589)
                     /gene="frlA"
                     /locus_tag="OO161_001063"
                     /pseudo
     CDS             complement(29261..30589)
                     /gene="frlA"
                     /locus_tag="OO161_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(30885..31052)
                     /locus_tag="OO161_001064"
     CDS             complement(30885..31052)
                     /locus_tag="OO161_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5462217.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         7282 a         7860 c         8568 g         7349 t
ORIGIN      
        1 ctgaccaaaa taatttgcga tttatttcat aggcgctggt aagcgcagca catcaggcca
       61 ttaagttgta aaatccggca gagccctctc tgccggacat actcattaat gaacttgcgg
      121 atctgccgga gacgcgttgt tgcggatttc agcaatatcc atcgcattga acagatagtg
      181 gttaccgcag taatcacaat gcatgtcaat ttcgccatct tccgccagga tgctatcaac
      241 ttcttcatca ggcagcgttt tcagcgcatc ggcgcaatgt tcacgcgagc aggtgcattt
      301 gaactccaca tcctgcggat cgtaaaccgt cacctcttct tcgtgataca aacgccacaa
      361 cacttcgttt gccggtaagg tcagcagttc ttcggttttg atggtttcgg ttagcgtcgc
      421 caggtggtca aagtcgtcct gctgggcatt ttgcgcaggc attacctgca acaacatacc
      481 gcctgcagcc ggtttgccgt ctacgtcgcc ggtgcgaata aacaggcgcg tcggcagctg
      541 ttcagaacgc ataaagtaat cttccaggca ggccgccagg gtatcacctt ccagaccaac
      601 tacgccctga tagcgttcgc cttcgctcgg ggtaatggtg atcaccacgt aaccattgcc
      661 gaccagcgtt ttcaggtcgg cattttctgg aatttcgccc tgcacgcgcg ccacaccgcg
      721 catttgctgg ttattgttac cgttaataac cgccagattc atcggaccgt cgccctgcag
      781 ctgtacggtg atatcaccat caaacttcag cgtagcggtt aacaggctgg tcgcaaccag
      841 cagttctgcc agcacgtttt taacgggctg cggataatcg tggttctcaa ggatctgttg
      901 cagggtttcc gaaacggtta ccagttcgcc gcgaacggca aaattttcaa acagatagcg
      961 atgtaattgg tcatgttgcg gcataatcat ctctcttgca ggtgacagtt attcactgtc
     1021 gccgtgttta aatcgtaaca ggtcgcggcg ctcttttttg tccgggcgtc ggtccgggtg
     1081 cggcatggtt aaggcattaa gtttacgtgc catcgccatt ttttcgcgtt tctctacact
     1141 ttccgcagtc tcttcataca gcaaggctgt ctcgctggcg gggcgacgct gttcagtaat
     1201 cgcctttaca atcaccgtgc gttcgtcatt tccctggcgc agagtgagcg tggcattcag
     1261 ctcgacgatt ttgctcggct tgctgcgctg cccgttgtaa tgcaccttac cgccttcaat
     1321 catttcacgg gccagcgcgc gggttttata aaaacgggca gcccatagcc atttatccag
     1381 tcgaacctca acagcaggtt tctctttcat ggcgtctcct tcacattagc gaggggatca
     1441 ggcggcggta gtcattcagt gacggatggc gttgatactg tttctcggca atcccggaat
     1501 caggattagt cacgccgagg cagtaacgaa taccaaattg cgcggcagca tcgagaatcg
     1561 cttcgctgtc atcaataaac agcgttcttt cagctttcag acccgtagct tcggccaccg
     1621 catgccataa ccgctgatcc tctttcggat aaccaaatgt gtgggtggaa agtaataaat
     1681 caaggtgtgc gtccagaccc gtatgctcaa gttttaccgc caggttgtgc ggatgagcat
     1741 tggtgagcaa aattcgctgc ttaccgctgg ctttcagtgc ctcaagaaac ggaatggtat
     1801 cttcacgcag tacggcgcgc ggtcccatct cggtggtcat tgcacagata tccagaccca
     1861 gttgctcact ccagtaatca agacagtacc agtttagcgt atgctgtacg tcgtgatatt
     1921 gctggcgcat atattccatc gcttcctgtg gcgtaacccc gtttttcgcg ccccatgttt
     1981 caggcaccag cttttgccag aaatagttat cgaaggcgag gtcgagcaac gtgccgtcca
     2041 tatccagcag aacggtatct acgtcctgcc aggcaatgtt gatatgcatg agggaaatct
     2101 ccagagtgaa gcaatttgcg cgacagggta gcataacctg ccgcgcaaac gtgttattcg
     2161 ataaggcttt ctgaaggggt gatcagttgc gggttcaggc agctttcata ataggcctga
     2221 atctccatca tgcgagtgcg atgacgctgg taacgacgcc aggcctgtac gccattgtaa
     2281 atggcgctac ccagcatcgt cagtagcagc aatgtggtgc tgagatagcg ccacaggccg
     2341 gaacgatccg ggatcggatg aaggccaata tgctgcgtac cattggcgtc agtgaagatt
     2401 tttgtgacga taccttcggc gttaaacggc gtatgcatca gcatttgtgc cagtttctgg
     2461 aaagcgttcc actgttcttg cggcgggtag tcgtaaagtg atgccgaagg ccagggctga
     2521 tcaacaaaat cgctgccttc gtcgctgaca atcaggaatc cgccgggggc cggactgttt
     2581 agtgattgtg ccgctcgcgc cgtttcatgc gtgataaacg gcgcggtgga ggttgccacc
     2641 aggttatcca gcgattccgc actcaccggg cgtaataaca cattcacgcc atcgagcttc
     2701 ccggcgttgg cgcgttttac cagcgcgtcc cagtctttac tgttgccgag attgaccagc
     2761 gcatttttca gtcgcacaca gtcatctttg gcagaacata aatccgctgt cttcagtaca
     2821 atgtcgccaa aatcatcaag caataccatg ccggattttt gaatcgctga acgtaatgag
     2881 gcactgacgc gagattcatc ttccggttta gggtgcagct ggcgattaac tgcttcagtc
     2941 aatgccgtcg ctttgttgac cagttcagat tctggtaatg gtaatgagcg ggcgtcattc
     3001 cagatgatct gcgagcagtc aaacggtaaa aaaggtgaat tggttttcgc gctccaggtt
     3061 ccggaagtgc gaatattaca cattcccgta ccgctaatac gcaatgtatc gcctacccgc
     3121 acgccagcgt cagccagttg ttttacgctg gtggcttcaa tggtctgcgc gcctttcatc
     3181 catgagagag tgaatttcag cggcatatcc agcgggatcc agaataacag cataaacagc
     3241 accagcagcg agcccgcagc gataatcgta ctgcgcagcc agtgctgtaa cggaaagttt
     3301 tttacttcat catgcagcga aagatatcgc ccctgacgca ctacgtggcg gtcgagatag
     3361 atatcaatat cggtttgttg atcgagatcc tgagcaatgt atggctgcca gtgtgcggga
     3421 tagaccaggt cgataatacc gagtgaaata ttgttgatct gttcctgatc gttttcgcca
     3481 aacaatcccc aacgacgggg tgtaccgcgc agacaatgaa tttcccgcag ggaggatttt
     3541 gccgggggcg cgaatagccc ccacagacct gcgcccagca gcagtaatgc gccgcctgcc
     3601 agccacggaa caaatacatc cggggtaatc aggcagaaaa agaacatcag gaaggaggcg
     3661 acgatcagca acgcttcacg cagcccgcgc ggacgactca gcgcatattc ttcatgcgtt
     3721 tctttgcgaa tattgagtag ctcgatttgc tcactctctt ccccgcgaat cgacgcctgc
     3781 gtggacggaa caggttgcaa ggcatagccg cgcgtttcct gcatgtactc ctgcagcgta
     3841 tgaccgttga gtgaaataac caacggcagc gaatcggtat gaatcagttc aacggtattc
     3901 tcatcgttga tgtactgttc ccagaagggg ggcaggtgga cttctaccga atcgaggtag
     3961 taacgccatt tattcggatc gtcggtagag atgccgtaac gtgtgatagc gtgtgtcagc
     4021 atcatgacgt tgttgctttc ggcattcagc gccagagaga tcggtgccgc gctagctccc
     4081 gttgggctag gcacctgtaa taccagcgtc aggctctcaa gataattttc aacggcgcta
     4141 cgttcttcag gtgtgagttt acgcgtttgc gcatccgcga aggcgttggt ccagggcagc
     4201 tgacgccgtc tggatcgcac ttttatcagc catcccgcaa gtagtgagca ggccagcaaa
     4261 gcagctaaaa aaatcacaat ggtgctcatg ctttccccat cttacttagt ctgtcaggcg
     4321 tggtcagtcg caccagacac aatacatgat tctgcggttg gctatcggca agcgtggagt
     4381 ttaacttgag catcattaag cacatcacag taaccgctga tcatagcaaa ccctctgaag
     4441 gcggaatatc agcaaattcc tggagttatt tcaacctatt gacttttaat ttgatttgag
     4501 gacgattatt tattaagtta cataaatgta aataacatgc aattcacatt gccgaaagcg
     4561 tgtccgatat cgcacaataa ccgctgattt cacagcatag atcccacgat gagcaaatca
     4621 ttacaaaaac ccaccattct gaatgttgaa actgtagccc gttcccgact gtttaccgtc
     4681 gagagcgtgg atctggagtt cagcaatggc gtgcggcgtg tttatgaacg aatgcgtcca
     4741 accaaccggg aagcagtgat gattgtgccg attgtggacg atcacctgat cctgatccgc
     4801 gaatacgcag tgggaactga atcctacgaa ttaggttttt cgaaaggatt aattgatccg
     4861 ggtgaaagcg tctacgaagc cgctaaccgc gagctaaaag aagaggttgg atttggtgcg
     4921 aacgatctga cttttttgaa gaagctcagc atggcaccgt cttacttttc cagcaaaatg
     4981 aatatcgtgg tcgcgcaaga tctctacccg gaatcgctgg aaggcgatga gccagagccg
     5041 ctaccacagg tgcgctggcc gctggcgcat atgatggatt tgctggaaga ccctgacttc
     5101 aacgaagcgc gtaatgtcag cgcgctgttc ctcgtgcgcg aatggttgaa agggcagggg
     5161 cgagtgtaaa tgcgtgaagt ttgcctgatg cacaacgctt atcaggctta tggggttcct
     5221 gcaatatatt gatttttcac gattttgtta gccggataac gcgttcaccc gcatccggca
     5281 taaacaaatg cactttgtca gccaactgaa aaggcgccga agcgcctttt taatcagaac
     5341 aattcctgtg cctcaccatt atcgataatg gtcgttccca cctcgtgcac cgcctgttgt
     5401 gtcggctgcg taccttcgat gaaatactct tcgcggctgt tgccaccatt agctaactgc
     5461 ccggtgctgc gatcgatatt caccgtcaca atacccggtg gcggcgtcag cggctgctcc
     5521 ggcacacctt caagaacggc tttcatataa gcgtcccatg caggctgggc actcttggct
     5581 ccgccttcgt aacctgagat ctgatcttta atcgctccgg aagccgttgt atgaccgaga
     5641 ttacgacggt gatcatcaaa gccaatccag accgaggtca caacgcccgg accgtaaccc
     5701 gagaaccacg catctttcga actgttagtg gtaccggttt tcccgccgat atcgcgacgc
     5761 tgcaaatcac gacctgcacg ccagccagta ccctgccagc ctggctcacc aaagatattg
     5821 gtgttcaaag cactcttaat caggaatgcc agcggagtgt tgatgacgtg cggtgcgtac
     5881 tcctgcgcgc cagtcttcgc cactaacgcc tgatttgcct gctccagctg cggcattggt
     5941 acagaaacat tctgctgctc gcgggagata gcgacatctt caacatcgtt attttccagc
     6001 acgttcgatt tctgcgtatc accgtaaatc accggaatat cgcattccgg gcaggctact
     6061 ttcggtttcg cttcgaaaat cacgccgccc tgatcgtttt caattttgct gataaaccac
     6121 gggtccacca ggaagccgcc gttcgccatg accgcgtagc cgcgcgccac ctgcattggg
     6181 gtgaaggacg ctgaacccag cgccagcgat tcggtgtgga caatgttttg cgccgggaag
     6241 ccgaagcgtt gcagatattc tgcagcgtag tcgacgccca tagcccgcat tgcacgtacc
     6301 atcaccacgt ttttcgactg acccagcccc tgacgtaagc gaattggacc agcatattgt
     6361 ggcggcgagt ttttcggctg ccagtcagaa ccggcacctg cgtcccagcg agaaattggc
     6421 acatcgttca acatacttgc cagcgtcaga cctttatcca tcgccgcggt gtagaggaac
     6481 ggtttgatgt tggaacccac ctgacgcagt gcctgggtgg cgcggttaaa cttgctctga
     6541 ttgaaatcaa agccaccgac cagcgccata acggcaccgt tttgcggatt gatcgacacc
     6601 agcgccgagt tcacttccgg cacttgtgcc agccaccatg catcgccaac ctgacgaacc
     6661 cagatttgct gacccgtttg cagaacatcg gtcactttac gcggcgtcgg tccctgctga
     6721 gtatccgaac ggtaaggacg cgcccagcga acgccttcca tactcaatgc gacggtcgac
     6781 ccgtccgcca gcatcgccgt cgctccctga ggattggcgc tggtgactgc ggcaggcagc
     6841 agcggaccat aggtcggcag cgccttcagc gtatcggtaa tcttgttgtt atcccacgcc
     6901 gactcgccca ctttccacag cacatttgcc gggccgcgat agccgtggcg catgtcgtag
     6961 tccagcacgt tattacgtac cgcctgctgt gcggcctgct gcactttgcg ggtgatggtg
     7021 gtgtaaatgc gataaccgtc ttcataggca ctttcgccat aacggttata catctcctgg
     7081 cgcaccattt cgctcaggta cggcgcagag aaagcaatct ccggcgcgtg atagttagcg
     7141 ttaatcgcct cagtgcgtgt ctgatcgaac tgttgttggg tgatataccc ttcatccagc
     7201 atccgcgaca gtacgacgtt acgccgcgcg acggcacgat ccattgagta gagcggatta
     7261 aaggtggaag gcgctttcgg cagcccggct atcacagcca tttcgttcag cgtcagttgg
     7321 tcgaccgttt taccgaaata gacttgtgcc gcagcaccga caccataggc gcggtaaccg
     7381 aggtaaatct tgttcagata aagctcgagg atctcgtctt tcgtcagcaa ctgttcaatg
     7441 cgaatcgcga ggaagacttc cttaatctta cgcatcagcg tgcgttctgg actgaggaag
     7501 aagtttctcg ccagctgctg ggtaatggta ctcgcccctt gtgacgcgtg gccggagaac
     7561 agcgccacac ttgccgcacg gaagatcccc accgggtcaa cgccgtgatg ctcgtagaag
     7621 cggctgtctt ccgtcgcgat aaaggctttc accatctccg gcgggatttg atccaacgta
     7681 accggaatac gacgtttctc accgtattga gcaatcagct cgccatcggc gctgtaaatc
     7741 tgcatcggaa tttgcaggcg aacatctttt aatgtcgcca catccggcag ttgtggctcg
     7801 atgtagcggt ataggccata aatcgagcct gctcccagca gaatgcaaca gactgcaagg
     7861 atcaaaaaat actttacgaa cttcactgga aatttctcat ttagtttcat ttgggcagtt
     7921 tataaacaaa cgcgcggtag tataaaggca agccagacgc attgatatac ccgtcagagt
     7981 gacgggtgat aaggagatca tcacaatggc atttaagatc tggcaaattg gtttgcattt
     8041 acaacagcaa gaagcggtag cggttgcgat cgtgcggggc gcaaaagaat gcttttcgca
     8101 acgctggtgg cggttgccgc tggagaacga cattatcaaa gatgggcgga ttgttgatgc
     8161 gcagcagctg gctaaaacgt tgttaccttg gagtcgcgaa ctgccgcagc gtcatcacat
     8221 tatgttggcg tttcccgcca gtcgcacatt acagcggtac agcggtcatt tccgcgcccg
     8281 tcgatgtccc ttggtgagcg ggagcaaacg gcctggctgt cagggacgat ggcccgcgag
     8341 ctggatatgg atccggactc cctgcgcttc gattacagcg aagactcact cagtcccgcc
     8401 tataacgtga ctgccgcgca aagcaaagag ctggcaacgc tgcttactct ggcagaaagg
     8461 ttgcgtgttc atgttagtgc gatcaccccg gatgccagtg cattacagcg attcctgcct
     8521 tttttacctt ctcatcagca atgtctggcc tggcgtgata acgaacagtg gctgtgggcg
     8581 acgcgctata gctgggggcg caaactggcg gtggggatga ctagcgcgaa ggagctggcg
     8641 gcagcgttat tcgttgatcc cgacagcgtc gcgatatgtg gcgaaggcgg attagacccc
     8701 tgggaggccg tttctgttcg tcagccgctg ctaccgccgc ctggtggaga ctttgccatc
     8761 gcgctggggc tggcgcttgg gaaggcgtac tgatgaaccc gccaattaat tttttgccct
     8821 ggcgacagca acgccggacc gcttttctgc gtttctggtt gctgatgttc gttgcgcctc
     8881 tgctgctggc cgtcgggata acgctaatac tgcgtctgac aggcagcgcc gaagcgcgca
     8941 tagacgcagt tttgcttcag gcggaacaac aactcgcccg cagcttacag ataacgaagc
     9001 cacgtttgct ggagcaacag cagttacgcg aacagcgttc gcaacggcag agccagcgac
     9061 aatttacccg cgactggcaa tctgcgctgg aagcactggc ggcgctttta cccgagcacg
     9121 cctggctgac aacgataagc tggcagcagg gaacgctgga gatcaagggg ctgacaacaa
     9181 gcattaccgc gttaaacgca ctagaaacgt cactccgcca ggatgcgcag ggacgctggc
     9241 aatttgagta tcagttaaca aggaaggtta gcgatgaaca tgttctttga ctggtggttc
     9301 gccacatcac cccgcctccg ccagttttgc tgggcattct ggttgctgat gttagttacg
     9361 ctcatttttc tgtcatcgac acaccatgaa gagcgcgacg cattaattcg attacgggca
     9421 agtcatcacc agcagtgggc cacactgtat cgcctggttg acaccactcc cttcagcgaa
     9481 gaaaaaacgc tgcccttttc gccactggat tttcagttgc ccggcgcgca actggtttcc
     9541 tggcatccat ccgcgcaggg aggtgagttg gcgttgaaaa cgctgtggga agcagtgccg
     9601 tcggcattca cacggctggc agagcgcaac gtcagcgtga gccgtttttc gttaagcgtg
     9661 gaaggtgatg atcttttgtt cacgctacaa ctggagacgc cgcatgaggg ttaaacgctg
     9721 gttgttggca ggtattgcat tgtgcctttt aaccggtatg cgtgaccctt ttaaaccgcc
     9781 ggaagatcta tgccggatta gcgaacttag ccagtggcgc tatcagggga tggtagggcg
     9841 aggcgagcgc atcatcggtg taataaaaga cgggcaaaag aaatggagac gggtgcagca
     9901 aaacgatgtg ctggaaaacg gctggacaat tttacagctg acgccagacg tactaacgct
     9961 gggtaccggg acaaactgcg aaccgccaca atggttgtgg caacggcaag gagatacaaa
    10021 tgaagcaatg gatagccgca ctactgttga tgctgatacc cggcgtacag gcggcaaagc
    10081 cgcaaaaagt gacgctgatg gtggatgacg ttccggtagc tcaggtgttg caggcgctgg
    10141 ctgaacagga gaagttgaac ctggtcgtgt cgccagacgt cagcggtacg gtgtcgttac
    10201 atctaacaga tgttccctgg aagcaggcac tacaaactgt agtgaaaagc gccggactga
    10261 taacgcggca ggaaggcaac attctctcag tgcattccat tgcctggcag aataacaata
    10321 tcgcccgcca ggaggcggag caggcgcggg cgcaggcaaa tctgccgctg gaaaatcgca
    10381 gtataaccct gcaatacgcc gacgcgggag aactggcgaa agcgggggag aagctactga
    10441 gtgccaaagg gagtatgacc gtcgataaac gcaccaatcg ccttttgcta cgagataaca
    10501 aaacggcgtt aagcgcgctt gaacagtggg tagcgcaaat ggatctgccg gtcgggcagg
    10561 ttgagctgtc ggcgcatatt gtcaccatta atgaaaaaag tttgcgtgag ttaggcgtga
    10621 aatggacgct ggccgatgcg caacacgctg gtggcgttgg gcaagtcacc acgcttggta
    10681 gcgacctctc cgtagcgacg gcgacaacgc atgtcggttt taacattggg cgcatcaacg
    10741 gacgcttgct ggatcttgag ctttccgcgc tcgaacaaaa acagcagctg gatattatcg
    10801 ccagtccgcg tctgctggcc tcacatcttc agcctgccag cattaaacag gggagcgaaa
    10861 ttccatatca ggtttccagc ggggaaagtg gcgcgacgtc ggtggaattt aaagaggccg
    10921 tcctggggat ggaggtcacg cccacggtgt tacaaaaagg tcgcatccgg ctgaaattac
    10981 acatcagcca gaacgttccg gggcaggtgc tacagcaggc cgatggcgaa gtgctggcga
    11041 ttgataagca ggagatcgaa acgcaggtcg aggtcaaaag cggagaaacg ttggcgctgg
    11101 gcggcatttt tacccgtaaa aataaatcgg gtcaggatag cgtaccgttg cttggcgaca
    11161 ttccctggtt cgggcaatta tttcgtcatg acggaaaaga atatgaacga cgcgagttag
    11221 tggtgtttat cacgccacga ctggtttcca gtgagtaaac agccgtaaaa gcggtaatgt
    11281 ttttatgctg aacgtgtttc atctatttga cgcgcgcagg tatttagcat acaaggagta
    11341 ccgatttgag agttggtgct cttcgctgcc tgcgttccat gatgatgatt tatcattcag
    11401 gcggcatttt gctgtctttt ttacgctaat cttacccggt gatttatcgc cagagcggtg
    11461 gtagcaaggc agcgcgcttg cagcgaccag atatgcagag ggatgggtga tttattcagt
    11521 tgccaaaccc gctggagtat tgagataatt ttcagtctga ctctcgcaat atcttatgag
    11581 gtttcagttc atgtcctgcg gcgctctctg agcgaagcgg gtttatcatt aacgaatagt
    11641 cttagtagta ccgaaaaaat ggcagagaaa cgcaatatct ttctggttgg gcctatgggt
    11701 gccggaaaaa gcactattgg gcgccagtta gctcaacaac tcaatatgga attttacgat
    11761 tccgatcaag agattgagaa acgaaccgga gctgatgtgg gctgggtttt cgatttagaa
    11821 ggcgaagaag gcttccgcga tcgcgaagaa aaggtcatca atgagttgac cgagaaacag
    11881 ggtattgtgc tggctactgg cggcggctct gtgaaatccc gtgaaacgcg taaccgtctt
    11941 tccgctcgtg gcgttgtcgt ttatcttgaa acgaccatcg aaaagcaact tgcacgcacg
    12001 cagcgtgata aaaaacgccc gttgctgcac gttgaaacac cgccgcgtga agttctggaa
    12061 gcgttggcca atgaacgcaa tccgctgtat gaagagattg ccgacgtgac cattcgtact
    12121 gatgatcaaa gcgctaaagt ggttgcaaac cagattattc acatgctgga aagcaactaa
    12181 ttctggcttt atatacactc gtctgcgggt acagtaatta aggtggatgt cgcgttatgg
    12241 agaggattgt cgttactctc ggggaacgta gttacccaat taccatcgca tctggtttgt
    12301 ttaatgaacc agcttcattc ttaccgctga aatcgggcga gcaggtcatg ttggtcacca
    12361 acgaaaccct ggctcctctg tatctcgata aggtccgcgg cgtacttgaa caggcgggtg
    12421 ttaacgtcga tagcgttatc ctccctgacg gcgagcagta taaaagcctg gctgtactcg
    12481 ataccgtctt tacggcgttg ttacaaaagc cgcatggtcg cgatactacg ctggtggcgc
    12541 ttggcggcgg cgtagtgggc gatctgaccg gcttcgcggc ggcgagttat cagcgcggtg
    12601 ttcgtttcat tcaagtcccg acgacgttac tgtcgcaggt cgattcttcc gttggcggca
    12661 aaactgcggt caaccatccc ctcggtaaaa acatgattgg cgcgttctac cagcctgctt
    12721 cagtggtggt ggatctcgac tgtctgaaaa cgcttccccc gcgtgagtta gcgtcggggc
    12781 tggcagaagt catcaaatac ggcattattc ttgacggtgc gttttttaac tggctggaag
    12841 agaatctgga tgcgttgttg cgtctggacg gtccggcaat ggcgtactgt attcgccgtt
    12901 gttgtgaact gaaggcagaa gttgtcgccg ccgacgagcg cgaaaccggg ttacgtgctt
    12961 tactgaatct gggacacacc tttggtcatg ccattgaagc tgaaatgggg tatggcaatt
    13021 ggttacatgg tgaagcggtc gctgcgggta tggtgatggc ggcgcggacg tcggaacgtc
    13081 tcgggcagtt tagttctgcc gaaacgcagc gtattataac cctgctcaag cgggctgggt
    13141 taccggtcaa tgggccgcgc gaaatgtccg cgcaggcgta tttaccgcat atgctgcgtg
    13201 acaagaaagt ccttgcggga gagatacgct taattcttcc gttggcaatt ggtaagagtg
    13261 aagttcgcag cggcgtttcg cacgagcttg ttcttaacgc cattgccgat tgtcaatcag
    13321 cgtaacaaca agaaaggtca ggccgcttat caagcggtct attagcttca ggttaattgc
    13381 aacgtggtaa gcattaacct tttagtgggg tgttaaatgg atgaattcaa accagaagac
    13441 gagctgaaac ccgatcccag cgatcgtcgt actggtcgtt ctcgtcaatc ttctgaacgt
    13501 tctgagcgta ctgaacgtgg cgaaccgcag atcaattttg atgatattga acttgatgac
    13561 actgacgatc gccgtccgac tcgtgcgcaa aaagagcgca atgaggaacc ggaaatcgaa
    13621 gaagaaattg acgaatccga agatgaaacc gtggatgaag agcgcgtaga gcgtcgtccg
    13681 cgtaagcgca aaaaagcagc cagtaaaccc gcttctcgtc agtatatgat gatgggcgtc
    13741 ggcattctgg ttctactgct gttgatcatc ggtatcggtt ctgcgctaaa agccccctcg
    13801 acctcttcca gcgatcaaac cgcgtctggc gagaagagta ttgatcttgc aggcaatgcg
    13861 accgatcagg cgaatggggt gcagccagcg ccgggaacca cgtctgcgga aaatactcag
    13921 caggatgttt ctctgccacc gatctcttct acgccgactc aagggcaaac cccggcggta
    13981 acggatggtc aacaacgtgt tgaagtgcag ggtgacctga acaatgcgct gactcagcca
    14041 caaaatcagc aacagttgaa caatgtggcg gtcaattcca cattgccgac cgaacccgcg
    14101 actgtcgcgc ctgttcgcaa tggcaatgca tcgcgtgaca cggcgaaaac gcaaaccgct
    14161 gaacgtccgt ccactacgcg cccagctcgt cagcaggcgg tgattgaacc gaaaaaaccg
    14221 caagcaaccg tgaaaacgga gccgaagccg gtagcacaga cgccgaagcg tactgaacca
    14281 gctgcccctg tggcgagcac gaaggcaccg gctgcgactt ctacgccagc accaaaagag
    14341 acggcgacta cggctccagt acagacggca tccccggcgc aaaccacggc aacaccagcc
    14401 gctggaggga agaccgcagg taatgttggt tcgttgaaat cggcaccgtc cagccattac
    14461 actctgcagc tgagcagttc ctctaactac gacaacctga acggttgggc gaagaaagag
    14521 aatctgaaaa actacgttgt ctatgaaacg acgcgtaatg gtcagccgtg gtatgtcctg
    14581 gtttctggcg tgtacgcttc gaaagaagag gcgaaaaaag cggtatctac attgccagca
    14641 gatgttcagg ccaaaaaccc gtgggcgaaa ccgctgcgtc aggtacaggc cgatctgaag
    14701 taatcaagct tatctcccgc aatggtttat cgttgcggga gctgcctgaa gcgctggatg
    14761 ctgtcggagc tttctccaca gccggagaag gtgtaattag ttagtcagca tgaagaaaaa
    14821 tcgcgctttt ttgaagtggg cagggggcaa gtatcccctg cttgatgata ttaaacggca
    14881 tttgcccaag ggcgaatgtc tggttgagcc ttttgtaggt gccgggtcgg tgtttctcaa
    14941 caccgacttt tctcgttata tccttgccga tatcaatagc gacctgatca gtctctataa
    15001 cattgtgaag atgcgtactg atgagtacgt acaggccgca cgcgagctgt ttgttcccga
    15061 aacaaattgc gccgaggttt actatcagtt ccgcgaagag ttcaacaaaa gccaggatcc
    15121 gttccgtcgg gcggtactgt ttttatattt gaaccgctac ggttacaacg gcctgtgtcg
    15181 ttacaatctg ctcggtgagt ttaacgtgcc gtttggccgc tacaaaaaac cctatttccc
    15241 ggaagcagag ttgtatcact tcgctgaaaa agcgcagaat gcctttttct attgtgagtc
    15301 ttacgccgat agcatggcgc gcgcagatga tgcatccgtc gtctattgcg atccgcctta
    15361 tgcaccgctg tctgcgaccg ccaactttac ggcgtatcac acaaacagtt ttacgcttga
    15421 acaacaagcg catctggcgg agatcgccga aggtctggtt gagcgccata ttccagtgct
    15481 gatctccaat cacgatacga tgttaacgcg tgagtggtat cagcgcgcaa aattgcatgt
    15541 cgtcaaagtt cgacgcagta taagcagcaa cggcggcaca cgtaaaaagg tggacgaact
    15601 gctggctttg tacaaaccag gagtcgtttc acccgcgaaa aaataattct caaggagaag
    15661 cggatgaaac agtatttgat tgccccctca attctgtcgg ctgattttgc ccgcctgggt
    15721 gaagataccg caaaagccct ggcagctggc gctgatgtcg tgcattttga cgtcatggat
    15781 aaccactatg ttcccaatct gacgattggg ccaatggtgc tgaaatcctt gcgtaactat
    15841 ggcattaccg cccctatcga tgtacatctg atggtgaaac ccgtcgatcg cattgtgcct
    15901 gatttcgctg ccgctggtgc cagcatcatt acctttcatc cagaagcctc cgagcatgtt
    15961 gaccgcacgc tgcaactgat taaagaaaat ggctgtaaag cgggtctggt atttaacccg
    16021 gcgacacctc tgagctatct ggattacgtg atggataagc tggatgtgat cctgctgatg
    16081 tctgttaacc caggttttgg cggtcagtcg ttcattccac aaacactgga taaactgcgc
    16141 gaagtacgtc gtcgtatcga cgagtctggt tttgacattc gactggaagt ggacggtggc
    16201 gtgaaggtga acaacattgg cgaaatcgct gcggcgggcg cggatatgtt cgtcgccggt
    16261 tcggcaatct tcgaccagcc agactacaaa aaagtcattg atgaaatgcg cagtgaactg
    16321 gcaaaggtaa gtcatgaata agtttgaaga tattcgcggc gtcgcttttg atctcgacgg
    16381 tacgctggtt gacagtgctc ccggtcttgc tgctgcggta gatatggcgc tgtatgcgct
    16441 ggagttaccc gtcgcaggtg aagaacgcgt tattacctgg attggtaacg gcgcagatgt
    16501 tctgatggag cgtgcattgg cctgggcgcg tcaggaacgt gcgactctgc gtaaaacaat
    16561 gggtaaaccg cccgttgatg acgacattcc ggcagaagaa caggtacgta ttctgcgtaa
    16621 actgttcgat cgctactata gcgaggttgc cgaagagggg acgtttttgt tcccgcacgt
    16681 tgccgatacg ttgggcgcgt tgcaggctaa aggcctgccg ctaggcctgg tcaccaacaa
    16741 accgacgccg ttcgtcgcgc cactgctcga agccttagat attgccaaat actttagcgt
    16801 ggttatcggc ggcgatgatg tgcaaaacaa aaaaccgcat ccggacccgc tgttactggt
    16861 ggctgagcgg atgggaattg ccccacaaca gatgctgttt gtcggcgact cacgcaatga
    16921 tattcaggcg gcaaaagcgg caggttgccc atcagttggc ttaacctacg gatataacta
    16981 cggcgaggct atcgatctca gccagcctga tgtaatttat cagtctataa atgaccttct
    17041 gcccgcatta gggcttccgc atagcgaaaa tcaggaatcg aaaaatgact aagcccatcg
    17101 tttttagtgg cgcacagccc tcaggtgaat tgaccattgg taactacatg ggtgcgctgc
    17161 gtcagtgggt aaacatgcag gatgactacc attgcattta ctgtatcgtt gaccaacacg
    17221 cgatcaccgt gcgccaggat gcacagaagc tgcgtaaagc gacgctggat acgctggcct
    17281 tgtatctggc ttgtggtatc gatcctgaga aaagcaccat ttttgttcag tcccacgtac
    17341 cagaacatgc gcagttaggc tgggcactga actgctatac ctacttcggc gaactgagcc
    17401 gcatgaccca gtttaaagat aaatctgcgc gttatgccga gaacatcaac gctggtctgt
    17461 ttgactatcc ggtgctgatg gctgcggaca tcctgctgta tcaaactaat ctggtaccgg
    17521 tgggtgaaga ccagaaacag cacctggaac tgagtcgcga tatcgcccag cgtttcaacg
    17581 cgctgtatgg cgagatcttt aaggtgccgg agccgtttat tccgaaatct ggcgcgcgcg
    17641 taatgtcgct gctggagccg accaagaaga tgtccaagtc tgacgataac cgcaataacg
    17701 ttatcggcct gctggaagat ccgaaatcgg tagtgaagaa aatcaaacgc gcggtcactg
    17761 actccgacga gccgccggta gttcgctacg atgtgcagaa caaagcgggc gtttccaacc
    17821 tgctggatat cctttctgcg gtaacgggcc agagcatccc agaactggaa aaacagttcg
    17881 aaggcaagat gtatggtcat ctgaaaggtg aagtggctga tgccgtttcc ggtatgctga
    17941 ctgaattgca ggaacgctat caccgtttcc gcaacgatga agccttcctg caacaggtga
    18001 tgaaagacgg cgcagaaaaa gccagtgtac acgcttcccg tacgctgaaa gcggtgtacg
    18061 aagcgattgg ttttgtggcg aagccgtaat atcaaaacgc tcggacggtc ccctcgcccc
    18121 tttggggaga gggttagggt gaggggagca ggcaagcgcc agtgggaatt accctttgct
    18181 cgcattgtcg aataaatcac ttcgtcatca ctctgaaacc ggggaaaccc ggttttttta
    18241 tcctcatttg caataatccg aaaaaatgtg aagcgcctcg ccgtttccac atctcacagt
    18301 tgccacttat cttcatttta atgaagatat aaacattcat tttattgaaa atatattatg
    18361 cgtcgaacgt ttatcaaaaa agaaggcgtc gtcatcacga cgctggcccg ttatttgttg
    18421 ggtgaaaaat gcggtaatcg actgaaaacc atagatgagc tggcaactga atgccgttca
    18481 tcggttggcc tgacgcaggc cgcgttgaaa acgttggaat caagtggagc gatacggatt
    18541 gaacgccgtg ggcgcaatgg cagttatctg gtcgaaatgg ataacaaagc gctgctgagt
    18601 catgtggata tcaataacgt tgtgtgtgcc atgcctttgc cctatacccg cttgtacgaa
    18661 ggtttagcaa gcgggctgaa agcccagttt gatggcattc ctttttatta tgcgcatatg
    18721 cgtggcgcgg atattcgtgt ggagtgtttg cttaacggtg tctatgacat ggcagtagtt
    18781 tcccgcctgg cggcggaaag ttatctcagc caaaacaatt tgtgcatcgc cctcgagctt
    18841 ggcccgcata cgtatgttgg tgaacatcaa ctgatatgcc gcaaaggtga gtctggaaat
    18901 gttaagcgag taggtctcga tagtcgttcg gcggatcaga aaatcatgac cgatgtcttt
    18961 tttggcgata gtgatgtgga acgtgtcgat ctctcttatc acgagagttt acaacgcatt
    19021 gttaaaggcg atgtcgatgc ggttatctgg aacgtggtgg cggaaaacga actgaccatg
    19081 ctgggattag aggcgacgcc gctcacagac gatccgcgat ttttacaggc caccgaagcc
    19141 gtggtcctga cgcgagtcga tgattaccca atgcaacaac tgctgcgtgc cgttgtagat
    19201 aaacacgccc tgctcgccca tcaacaacgg gtagtgagtg gggaacagga accgagttat
    19261 taaacgaaag gcatctgata tggaaaccag actcaacctg ctttgcgagg caggcgttat
    19321 tgataaggac atctgcaaag gcatgatgca ggtcgtcaac gtactggaaa cagagtgcca
    19381 tctgccggtg cgcagtgagc aaggaacgat ggcgatgaca catatggcga gtgcactgat
    19441 gcgcagtcgc cgtggtgaag aaatagagcc gctggataac gaattgctgg cagagctggc
    19501 gcaatccagc cactggcaag ccgttgtgca attgcatcag gtgttgttga aggaattcgc
    19561 actggaagtt aatccgtgtg aagaaggcta tttgctggcc aacctttatg gattatggat
    19621 ggctgctaac gaagaggttt gaagctcccg acaaatccac gtcgcacaac ttaaatattc
    19681 aaaaaaatga atatttaatt caaggaacag aaacgaggca acaagatgtt tgtagaagca
    19741 ttgaaacgcc agaacccggc gctgatttcc gccgcactaa gtttgtggca gcagggcaag
    19801 atcgccccgg acagctgggt gatcgacgtg gatcaggtac tggaaaacgg taagcgactg
    19861 attgagacgg cgcggcttta cggcattgaa ctgtatctga tgaccaagca gtttggccgt
    19921 aacccatggc tggcggaaaa attgctggca ttaggttaca gcggcattgt ggcggtggat
    19981 tacaaagagg cgcgagtcat gcgccgcgct ggtttgtcgg tggcgcatca ggggcatctg
    20041 gtacaaatcc cttgtcatca ggttgctgac gccgttgaac agggcaccga cgtcatcacc
    20101 gtgtttactc tcgacaaagc gcgggaagtt tctgcggcag cggtgaaggc cggacgtgtg
    20161 cagtttgtgc tgctcaaagt ttatagcgac gatgattttc tttatccggg gcaggagagc
    20221 ggttttgtac agcattcgct gcacgaggtg gttgctgaaa ttaagaaact gccgggtctg
    20281 catttagccg gacttaccca tttcccttgc ctgctttggg atgaggctgc cggaaaagtt
    20341 ttgccgacac cgaatcttca cacgctgata caggcacggg atcaactggc gaaatctggt
    20401 attgcacttg agcaactgaa cgcgccttca gcgaccagct gcacttcgct gccattactg
    20461 gcgcaatacg gtgtgactca tgccgaaccc ggtcatgcgc tgacgggcac tattccggca
    20521 aaccagcagg gcgatcagtc tgaacgtatc gcgatgctct ggttgagtga aatctcccat
    20581 catttccgtg gcgacagcta ctgctacggc ggcggttact accgtcgtgg tcatgcgcaa
    20641 catgcgttgg tgtttacgcc agaaaatcaa aagattactg aaaccaatct caaaactgtg
    20701 gatgacagca gtatcgacta caccctgtcg ctggcaggcg agtttccggt aagcagtgca
    20761 gtggtgctct gtttccgcac gcagattttt gtcacccgta gcgatgtggt gctggtgtcc
    20821 ggtattcacc gtggcgaacc gaaaatcgtt ggtcgttatg acagtcttgg aaactctctg
    20881 ggggcgtaat ggcgcgattt gtggtgttag tgattgatag ctttggcgta ggggcaatga
    20941 aagatgtcac gctggtgcgt ccgcaagatg cgggagcgaa tacatgtggt cacatcctga
    21001 gccagttgcc gcatttgcag ctaccaacgc tggagaagct ggggctaatc aacgcattgg
    21061 gttatgcgcc aggcgatatg cagccgtcag attccgcaac ctggggcgtg gcagagctgc
    21121 aacatgaagg tggcgatacc tttatggggc atcaggaaat tttaggcacg cgcccgttac
    21181 cgccgctgcg gatgcctttt tgcgatgtga ttgaccgtgt tgagcaggca ttagtatccg
    21241 ccggttggca ggtggagcgc cgtggcgatg aactgcaatt tctgtgggtc aatcaggcgg
    21301 ttgcgattgg cgataatctc gaggcggatt taggccaagt ctataacatt accgccaatc
    21361 tctctgtgat ctcttttgac gacgcaatca aaattggtcg tatcgtgcgt gagcaggtac
    21421 aggtcggtcg ggtcattaca tttggtggcc tgttaaccga cagtcaacgc attctcgatg
    21481 ccgcagaaag caaagaaggg cgctttattg gtatcaatgc gccgcgttct ggcgcttatg
    21541 acaacggctt ccaggtcgtg catatgggct acggtgtcga taaaaaagtg caggtgccgc
    21601 aaaaactgta tgaggcgggc gtgccaaccg tgctggtggg taaggtggca gatatcgtca
    21661 gcaatcctta tggcgtgagc tggcaaaacc tggtggatag ccagcggatt atggatatca
    21721 ccctcgacga atttaacacc catccgacgg cgtttatttg caccaatatc caggaaaccg
    21781 acctcgctgg tcatgcagaa gacgtcgcac gttatgccga acgtttgcag gtcgttgacc
    21841 gtaaccttgc ccggcttgtt gatgcgatgc agccagatga ttgcctggtc gtgatggcgg
    21901 atcacggcaa cgatccgacc attggtcaca gccaccatac ccgcgaagtg gtgccgctgc
    21961 tggtttatca gcaaggcctg gctgctacgc agctcggtgt gcgcaccacg ctttctgatg
    22021 tgggagctac cgtgtgtgaa tttttccgtg cgccaccgcc acaaaatggt cgctcttttc
    22081 tttcctccct ccggtttgca ggagacaccc tatgagtttt gatccgacgg gttacaccct
    22141 ggcccatgag catctgcata ttgatctctc cggctttaaa aacaacgtgg actgccgcct
    22201 tgatcagtat gcgttcattt gccaggagat gaacgacctg atgacccggg gtgtacgtaa
    22261 tgtgattgag atgaccaacc gttacatggg gcgcaatgcg caatttatgc tcggtgtaat
    22321 gcgcgaaacc gggatcaacg tggtggcctg tactggttat taccaggatg cgtttttccc
    22381 ggaacatgtg gtgacccgca gcgtgcagga actggcgcag gagatggtcg atgaaattga
    22441 acagggtatc gatggcacgg agctgaaagc cgggctcatc gcggagatcg gctccagcga
    22501 aggaaagatt acgccgctgg aagagaaggt atttattgcc gctgcgctgg cgcataacca
    22561 gaccggacgc ccgatctcca cgcatacgtc gttcagcacc atggggctgg aacaactggc
    22621 tctgttgcaa gcccacggag ttgatctttc gcgcgtcacc gttggtcact gcgatctgaa
    22681 agacaacctc gacaacattt tgaagatgat cgatctcggc gcgtacgtgc agttcgacac
    22741 cattggtaag aacagttact acccggacga aaagcgcatt gcgatgctcc acgcgctacg
    22801 tgaccgtggg ttgctgaacc gcgtcatgct gtcgatggat attacgcgcc gctcccattt
    22861 aaaagccaat ggtggttatg gctatgacta tttattgacc acctttattc cgcaattgcg
    22921 ccagtcagga ttcagtcagg ccgatgtgga tgtgatgtta cgtgaaactc cctctcaatt
    22981 tttccaataa ggacagactc atgaaaaaga ttggcgttgc aggcttacag cgtgagcaga
    23041 ttaaaaaaac cattgaagcg acggctcctg gcagttttga agttttcatt cacaacgaca
    23101 tggaagcggc aatgaaagtg aaatccgggc aactggatta ctacatcggt gcgtgtaata
    23161 ccggtgcggg cgcggcattg tcgattgcca tcgcggtgat tggctataac aaaagttgca
    23221 ccattgccaa accaggcatt aaagcgaaag acgagcatat cgccaaaatg atcgctgaag
    23281 gaaaagtggc gtttggcctt tccgttgagc acgtcgaaca cgcgattccg atgctgatta
    23341 accatctgaa ataaaaggca cgactatgga tctgtatatt cagattatcg tggtggcgtg
    23401 cctgacgggt atgacatcgc ttctggcgca tcgctcggcg gctgtttttc atgacggcat
    23461 ccgcccgatc ctgccgcaac tgattgaagg ctatatgaac cgtcgcgagg cggggagtat
    23521 cgcttttggt ctgagcattg gttttgtggc ctcggtgggg atctctttta ccctgaaaac
    23581 cgggctgctc aacgcatggt tactctttct tcctaccgat atcctcggcg tactggcgat
    23641 aaacagcctg atggcgtttg gtcttggcgc tatctggggc gtgttgatcc ttacttgcct
    23701 gttgccagta aaccagctgc tgaccgcgct gccggtggat gtattaggta gcctcgggga
    23761 attaagctcg ccggtagttt ctgcttttgc actgttcccg ctggtggcga ttttctacca
    23821 gtttggctgg aagcaaagtc tggtcgccgc cgtggttgtt ctgatgaccc gtgtggtagt
    23881 cgtgcgctat ttcccacatc ttaaccctga atccatcgaa atctttattg gcatggtgat
    23941 gctgctgggg atcgcgataa ctcacgacct gcgtcatcgt gatgaaaatg atattgatgc
    24001 cagcgggctt tcggtgtttg aagaacgcat gtcgcggatt atcaaaaact taccctatat
    24061 cgccatcgtg ggagcattga ttgccgccgt tgccagcatg aagattttcg ccggcagtga
    24121 agtgtcgatc ttcactctgg agaaagcgta ctccgcaggc gtaacgccgg aacaatcgca
    24181 aacgctgatc aaccaggcgg cactggcaga atttatgcgc gggctgggtt ttgtaccgtt
    24241 gattgccacc accgccttag caacgggtgt gtatgcagtt gcgggcttta cctttgttta
    24301 tgcggtgggc tatctctcgc cgaatccgat ggttgcagcg gtattaggcg cagtggttat
    24361 ttcggcggaa gtcttgctgc ttcgttctat cggcaaatgg ctgggacgct acccgtcggt
    24421 gcgtaatgcg tcggataaca tccgtaacgc catgaatatg ctgatggaag tggcgctgct
    24481 ggtcggttcg attttcgcag caattaagat ggcgggttat accggattct ctatcgcggt
    24541 tgccatttac ttcctcaacg aatccctggg ccgtccggta cagaaaatgg cggcaccggt
    24601 cgtggcagta atgatcaccg gtatactgct gaatgttctt tactggcttg gcctgttcgt
    24661 tccggcttaa ggaaggcacc tatgaagacg tttactctgc aaagcctgac gcttattgag
    24721 gcgcagcaaa agcagtttgc gctggtggac acgatttgcc gtcactttcc cggcgcggag
    24781 tttctaacca gcggtgattt gggcttaacg ccggggctga atcaaccgcg tattacccaa
    24841 cgggtggagc aggtgctggc tgatgcattt cacgcacagg ctgcggcgct ggtgcagggc
    24901 gcggggactg gcgcgattcg cgccgggctg gcagctttgc tcaaaccggg gcagcgtctt
    24961 ctggtgcatg acgcgcctgt ttacccgacg acacgggtta ttattgagca gatggggctg
    25021 acgcttatta ctgttgattt caatgacctg tcggcactga agcaggtcgt cgacgagcaa
    25081 caaccggatg cggcgctggt gcagcatacg cgccagcagc cgcaggacag ctacgtgctg
    25141 gcagatgtgc tggcaacgtt gcgcgcggca ggtgttccag cgttaaccga tgacaactat
    25201 gcggtgatga aggtggcgcg aatcggctgt gaatgcggcg cgaatgtctc gacattttcc
    25261 tgcttcaagc tatttgggcc agagggtgtt ggtgcagtgg tcggcgatgc tgatgttatc
    25321 aaccgtattc gcgccacgct ttactccggc ggtagccaga tccagggcgc acaggcgctg
    25381 gaagtattgc gttgtctggt gtttgcgcca gtgatgcacg cggtgcaggc aggggtatct
    25441 gaacggttgc tggctttgct taacggtggt gcggtgccgg aagtgaaaag cgcggtgatt
    25501 gctaatgcgc agtcgaaggt gttgattgtc gagtttcatc agccgattgc cgccagagtg
    25561 ctggaagagg cgcaaaagcg cggtgccttg ccttacccgg tgggtgcaga gtcgaaatat
    25621 gaaatcccgc cgctctttta tcgcctttcc ggaacgtttc gccaggcgaa tccacaatca
    25681 gaacattgtg cgattcgcat taacccgaat cgcagcggtg aagagacggt gctgcggatt
    25741 ttgcgtgaga gtattgccag tatttaatgc tggatagcac cgtcaagcta aattccgtac
    25801 tgaacggtcc cctcgcccct ttggggagag ggttagggtg aggggaatgt acgggatgta
    25861 aaattcaacg cgccagcatc gccggatgcg atgctggcgc atcttatccg gcctacggtt
    25921 aatgtctttt attatcaata gttaaggtga tgcgattggc gcggcagaag agttcggaga
    25981 cgtgcaccgg tttgtcattc tgatcaaagg cgattttgct gatgcgaaac aacggttcgc
    26041 ccagttcgca tttcagccat tttgcctgcg ggcgggtggc ggcaaagata tcgatggtct
    26101 ttttgtcgct gaccactcgc gtgtcaaaac gctcctgaaa taactgatag gtggagcttc
    26161 cttcgacgta aatctcatca aagtcaggat aacgcgacag cgggatccat gaactatcaa
    26221 taaacagcgg ctctttatcg agatacatca cccggcagag atggaacact tcgctgtttc
    26281 cggggatgtt cagcttttcg caaaacggcg cggcgctgac ccgttcctgt tcgatcactt
    26341 tctctttcgt cgccttgcct tgtgagacgc caaaatcggt aaaaccactg acagtaagca
    26401 gggcgttttc aactttctgg ctttgtacaa aggtgccttt tccttgccag cggatcagta
    26461 cgccgtccgc cactaagtcg ctgatggctt tgcgaatggt aatgcggctg acgttatatt
    26521 gtgtacaaag ctcgttttcg gtagggatct gttgcccggc ctggtaaacc ccctgcgcga
    26581 tatcatccag cagtcgctgg cggacggtag cgtagaggag ttgatgagag tagcggtccg
    26641 tagctgacat aatcgaccct gaaataaaga atgaattact cggattatag ggtcgttttc
    26701 cggcaagtaa cagcacgttg gcgtgaagat gctcacgcca acgttatacc taccaggcac
    26761 cgtggtactg aatggttttc gccgcgcacg ccgttccctg cgccatcgcc tgcggtaatg
    26821 tcatccccgc agaccagccg caaaggaatc cggcaatgaa cgaatctccg gcacccatgg
    26881 tgtcgataac cgtcaccggt tcaggagcct gacgccagaa ctgcgcgcca tcccaggcaa
    26941 tgctgccgtt ttcatccagc gtgacaatca ctgttcctgc gccacgggca acaatcgctt
    27001 tcatcttcag acgcagcgtt tcgtcttctt gcggtgcgga ggcaaaggca aaatcgagat
    27061 gcggcaccag tgtctgccag agcgggctgt cccacttgtc ggagaagtcg aaagcggtaa
    27121 gtttgcccgc agcgtgcagc tgtgggaatg cgtcttccgc atgtccccag attgccgcgt
    27181 gcacaatgtc atactgcgcc agccaggcgt aatcctcttc actcagggca aagtcggcca
    27241 tcacgccttc ggtgtagtcg ccaaaaacgc gatcattgtc gtgcagttcc acctgagttt
    27301 gtgcggtaac gccgtgtttc gtatggacat ggctgatatc gacgcccatg cgggcgagat
    27361 cctgcttcag ctttgtgccg tagtcatcgt cacccaccca ggtaatgcat cccggctgta
    27421 tgccgtagcg agtgcagtac accgccacat tgaccgcatt accgccagaa aacgctttat
    27481 tcagttgcgg gtagatatcg acgcagttat cgccgattgt cgccagggtt ttcatctctc
    27541 atcctccggc agcagcgcgc gaaagcgttc cagcgcctgg cgggcataga gtctgggctc
    27601 gttcatatac atcgtcacca gctccaccgt acagtaaccc tcatagcccc gctcaataat
    27661 atcgcgcatc agttcccgca gcggcatttt gccttctcca ggaatgtaat gcgtgtcgct
    27721 ggccccgtcg ctgtcgacaa tatgcagatg acgtaattta tcgcccagtt tgtcgaaata
    27781 actcatcacc ggttccgcct ggacatacgg cgcgcaaatg tcgaccatgc tgaacaggcg
    27841 cggcgaaggc accagcgcca gcgcatgaag cacatcatta gcattacaca cgacgttcga
    27901 ttcatacggc gttaacggtt cgaggattag atccatgccg atgttttccg cgtactcata
    27961 cagttcgctt aggttctctg ccagccgtcc ccagataaca ttaggtggcg tgagatagcc
    28021 cgcgtgggcc gcggaaatca gcgtataacc cgcgttcatc tcttttgcca tatccatcgc
    28081 cagcttgatc atgtcgaggc tttcgcgacg catatgttca tcgcccagca tcatgttata
    28141 cggatagccg ttggtttctg gcgtatagcc gataatcggc atctgatacg tctgcgccag
    28201 cgccttgatt tgtttgatgc cgcccgcttt taagtccggc gcgaacgcgt gcgggcgacc
    28261 gccccaaatt tcgatgccgt cgtaacccag ctcgcttgca tcacgaaatg cgtgttcaat
    28321 aggcagccgc tggtggccgc aggtaaacat acctgttttc atcgggtaac tccttgcgta
    28381 aatccggccc gttgccgggc cgggcgaggg attaatattc caccagacca ccgtaatagc
    28441 ggcgttcatc cgggttgtga tctttgtaaa tagacaggta gtagcagagc cactccattg
    28501 gcacgaacat caggaacggt gccagccacg ggtgcagccc ttgcgaaatt tcggcgtaat
    28561 cgatgacgat cacgttgtca gtacgctgtt taacaaagtt aatggcgcgt tcggtggtgt
    28621 ggcgactttc atcattgccg agcaggaaca ggaacggaac gcccggttcg acaatctcca
    28681 gcgggccatg gcggaactct ccgctctcaa tcacgcagcc gtgcgtccag gtaaattcca
    28741 tcagcgttac aatgccttct ttgtaaccca gcggacgcag cggacccgca gcaacggtat
    28801 aaatcatcgg ccactggctg gccagttcac caagctggcg gcctttttct tcccaggtgc
    28861 gtaccagatg accgagcgca ttcggcaact gcttgagatc gtttttgatc ttgccgattt
    28921 ccgcgttcgg cgcgaggcgg gtgatcatct ccagcaccac gctgtagcag agcagcaggt
    28981 gaatttccca gatacagtcg gcctgataat caatgctaaa ttccgccgcc gaggtaatcg
    29041 ggctgtccgc gcgtttggtg aacgccgcag tgagtgcgcc gcaggcccgg cccagctcca
    29101 gcgctttgat tacctcttcc gttttaccgt agtcagaaac gccaatcacc gcgcaacgat
    29161 cgtcgaggcg atacggggtg ttatcgcaga actcccagcc ggaaatggcg tagacctgaa
    29221 gatcggaaaa gcgatccgcc aaatgtttcg ccgcttgcgc cgcgttgagc tggcggctac
    29281 gcttcgccca gaaggcgtaa gcaggcagac cagtagcaat aacgatgacg gcgcagataa
    29341 ggccgggaat cggtgcccag acaaaggttg aggcgaccag aatgaggctt gacgcaatgg
    29401 cgagggtggt catcagcccg aaagccggag tacgccacag cggtttgtaa tcgtcgcgtt
    29461 tacgacacca gatgatggag ccgaaggtga gggtattttt gaaacacatc accagggtga
    29521 aataacccag caggctggtg agatcggaaa cgaagatgaa gaagagcccc agcgcccctt
    29581 gcaggatgat ggagacatcc ggcgtgttgt atttcggatg cacatggccg aagcatttaa
    29641 agaacaggtt gtctttcgcc atcgcgtatt ccagccgcgg ctggtacatc acgcagctgg
    29701 aaagcgaacc aagaatgacg atcatcgccg tgatggcaac aaagatccca gcggtgctgc
    29761 cgagtgcggg gatccaggtc agggcgtcgg aaatcggcgt ttcagaattg gcgagtttgt
    29821 cgaagggcat caggtcggaa atcaccagcg ccagcagggt gtagagcacc agaaccagca
    29881 gacaggaacc aatcagcgct agtggcatgg tttttccggg gtttttaatt tcgccggtca
    29941 tataacagat agaggccatg ccggtatacg accaactggt ggcagagatc cccgccagca
    30001 gcgccataaa gctgcccgtt gcgccaatcg cagtggtggt aggggcggca aaattctccg
    30061 ctttaaacta gaagatccca aggccaatga cgatagtgaa cgggataatt ttggcgatgg
    30121 taattagcgt ctgaaacgct gcgccgcctt caacggagcg caggtgtagc aacataaagg
    30181 cgataattaa tccggcggcg ataaatttac cgggcaacgg atcgataggc gttaaaaagc
    30241 caagattgct gacaatcgcc agcgccataa tcgacaatga cggcgcatcg ttggcccaga
    30301 agctggccca gccggagagg aaagccagcg gtcggcttcc ggcatttttc agataaacat
    30361 aatctgcgcc attttccgga taagcggtgg atagttccgc atagacgcac atttgcggga
    30421 tcacaattaa cccgccaatg acaaacgtga gcaccgtaag ccacggcgtg cccgctgctt
    30481 ttgccacttc acccacagat acaaaaatac cggagccgac ggttgtcccg acggcgattg
    30541 caagaacggc ccaaaatccg agcttgcgtt ggagttcctg gcttcccata gcgatacctt
    30601 tttcttgtcc aacacgttgt aggggagagt ttgcagtcat ttaaaaggta acatgacaat
    30661 gcatgatgaa tataataaca tatgatgtta tgtaatgcgg aggaagatca caaaatttcg
    30721 cacaggatcg ctctgtggct aatgaatgta gttatccaat tgaaattaaa gtgaaaatat
    30781 ttttacgggt ggggagcaag aaggatatag aaacaaatac gccctcggaa aatccagagg
    30841 gcgtcgggca attaaaccgg tgttagccga tttctgtcag agacttactg tgcagtagga
    30901 ccgcaaccgc caatgatttt agaaatagaa attgccgggt gcagcaggta gtcgtaagag
    30961 cagttcttat cacggttttc aatatgacca gtacatgcag ttaacgtagc cagtactgca
    31021 cctaccagtg caactttaat aaatttgttc atttacatt
//
LOCUS       DAJTNA010000027        30918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTNA010000027 DAJTNA010000000
VERSION     DAJTNA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30918
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.027"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..75
                     /locus_tag="OO161_001065"
     CDS             <1..75
                     /locus_tag="OO161_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462218.1"
                     /translation="DGLRFAIREGGRTVGAGVVAKVLS"
     gene            305..688
                     /gene="secE"
                     /locus_tag="OO161_001066"
     CDS             305..688
                     /gene="secE"
                     /locus_tag="OO161_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5462219.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            690..1235
                     /gene="nusG"
                     /locus_tag="OO161_001067"
     CDS             690..1235
                     /gene="nusG"
                     /locus_tag="OO161_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5462220.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1395..1823
                     /gene="rplK"
                     /locus_tag="OO161_001068"
     CDS             1395..1823
                     /gene="rplK"
                     /locus_tag="OO161_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5462221.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1827..2531
                     /gene="rplA"
                     /locus_tag="OO161_001069"
     CDS             1827..2531
                     /gene="rplA"
                     /locus_tag="OO161_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5462222.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2944..3441
                     /gene="rplJ"
                     /locus_tag="OO161_001070"
     CDS             2944..3441
                     /gene="rplJ"
                     /locus_tag="OO161_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5462223.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3508..3873
                     /gene="rplL"
                     /locus_tag="OO161_001071"
     CDS             3508..3873
                     /gene="rplL"
                     /locus_tag="OO161_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5462224.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4193..8221
                     /gene="rpoB"
                     /locus_tag="OO161_001072"
     CDS             4193..8221
                     /gene="rpoB"
                     /locus_tag="OO161_001072"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5462225.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8298..12521
                     /gene="rpoC"
                     /locus_tag="OO161_001073"
     CDS             8298..12521
                     /gene="rpoC"
                     /locus_tag="OO161_001073"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5462226.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12734..13273
                     /locus_tag="OO161_001074"
     CDS             12734..13273
                     /locus_tag="OO161_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HCR5462227.1"
                     /translation="MKQEVEKWRPFGDPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13683..14816)
                     /gene="thiH"
                     /locus_tag="OO161_001075"
                     /pseudo
     CDS             complement(13683..14816)
                     /gene="thiH"
                     /locus_tag="OO161_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
     gene            complement(14813..15583)
                     /gene="thiG"
                     /locus_tag="OO161_001076"
     CDS             complement(14813..15583)
                     /gene="thiG"
                     /locus_tag="OO161_001076"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5462228.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15585..15785)
                     /gene="thiS"
                     /locus_tag="OO161_001077"
     CDS             complement(15585..15785)
                     /gene="thiS"
                     /locus_tag="OO161_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5462229.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15769..16524)
                     /gene="thiF"
                     /locus_tag="OO161_001078"
                     /pseudo
     CDS             complement(15769..16524)
                     /gene="thiF"
                     /locus_tag="OO161_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            complement(16517..17152)
                     /gene="thiE"
                     /locus_tag="OO161_001079"
     CDS             complement(16517..17152)
                     /gene="thiE"
                     /locus_tag="OO161_001079"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5462230.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17152..19047)
                     /gene="thiC"
                     /locus_tag="OO161_001080"
     CDS             complement(17152..19047)
                     /gene="thiC"
                     /locus_tag="OO161_001080"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5462231.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQLQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSIKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19128..19228)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19281..19757)
                     /gene="rsd"
                     /locus_tag="OO161_001081"
     CDS             complement(19281..19757)
                     /gene="rsd"
                     /locus_tag="OO161_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5462232.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19852..20625
                     /gene="nudC"
                     /locus_tag="OO161_001082"
     CDS             19852..20625
                     /gene="nudC"
                     /locus_tag="OO161_001082"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5462233.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20665..21729
                     /gene="hemE"
                     /locus_tag="OO161_001083"
     CDS             20665..21729
                     /gene="hemE"
                     /locus_tag="OO161_001083"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5462234.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21739..22410
                     /gene="nfi"
                     /locus_tag="OO161_001084"
     CDS             21739..22410
                     /gene="nfi"
                     /locus_tag="OO161_001084"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5462235.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22453..23043
                     /locus_tag="OO161_001085"
     CDS             22453..23043
                     /locus_tag="OO161_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5462236.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23230..23502
                     /gene="hupA"
                     /locus_tag="OO161_001086"
     CDS             23230..23502
                     /gene="hupA"
                     /locus_tag="OO161_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5462237.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23515..24210
                     /locus_tag="OO161_001087"
     CDS             23515..24210
                     /locus_tag="OO161_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5462238.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24212..24637)
                     /gene="zraP"
                     /locus_tag="OO161_001088"
     CDS             complement(24212..24637)
                     /gene="zraP"
                     /locus_tag="OO161_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5462239.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24875..26251
                     /gene="zraS"
                     /locus_tag="OO161_001089"
     CDS             24875..26251
                     /gene="zraS"
                     /locus_tag="OO161_001089"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5462240.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26248..27572
                     /gene="zraR"
                     /locus_tag="OO161_001090"
                     /pseudo
     CDS             26248..27572
                     /gene="zraR"
                     /locus_tag="OO161_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(27569..28858)
                     /gene="purD"
                     /locus_tag="OO161_001091"
     CDS             complement(27569..28858)
                     /gene="purD"
                     /locus_tag="OO161_001091"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5462241.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28870..30459)
                     /gene="purH"
                     /locus_tag="OO161_001092"
     CDS             complement(28870..30459)
                     /gene="purH"
                     /locus_tag="OO161_001092"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5462242.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7315 a         7985 c         8443 g         7175 t
ORIGIN      
        1 gacggtctgc gtttcgcaat ccgtgaaggc ggccgtaccg ttggcgcggg cgttgtagca
       61 aaagttctga gctaattgcc gataacattt gacgcaatgc gcactaaaag ggcatcattt
      121 gatgcccttt ttgcacgctt tcgtaccaga acctggctca tcagtgattt tctttgtcat
      181 aatcattgct gagacaggct ctgttgaggg cgtataatcc gaaaagctaa tacgcgtttc
      241 gatttggttt gcctcgcgat ctcggggtga aaatgtttgt agaaaacttc tgacaggttg
      301 gtttatgagt gcgaataccg aagctcaagg aagcgggcgc ggcctggaag cgatgaagtg
      361 ggtcgttgtg gtggcattgc tcttggtggc gattgtcggc aactaccttt atcgcgacat
      421 tatgctgccg ctgcgtgcgc tggccgtagt aattctgatt gctgcagcgg gtggtgtcgc
      481 gctgttaacg acaaaaggta aagctaccgt tgcttttgcc cgtgaagcgc gtaccgaagt
      541 ccgtaaggtc atttggccga ctcgccagga aacattgcac accacgctga ttgtggctgc
      601 ggttaccgca gtaatgtcac tgatcctgtg gggactggat ggtattctgg ttcgcctggt
      661 atcctttatc actggcctga ggttctgaga tgtctgaagc tcctaaaaag cgctggtacg
      721 tcgttcaggc gttttccggt tttgaaggcc gcgtagcaac gtcgctgcgt gagcatatca
      781 aattacacaa catggaagat ttgtttggtg aagtcatggt accaaccgaa gaagtggttg
      841 aaatccgtgg cggtcagcgt cgcaaaagcg aacgtaaatt cttccctggc tacgtcctcg
      901 ttcagatggt gatgaacgac gcgagctggc acctggtgcg cagcgtaccg cgagtgatgg
      961 gcttcatcgg cggtacttcc gatcgtcctg cgccaatcag cgataaagaa gtcgatgcga
     1021 ttatgaaccg cctgcagcag gttggtgata agccgcgtcc gaaaacgctg tttgaaccgg
     1081 gtgaaatggt ccgtgttaat gatggtccgt tcgctgactt caacggtgtt gttgaagaag
     1141 tggattacga gaaatctcgt ctgaaagtgt ctgtttctat cttcggtcgt gcgaccccgg
     1201 tagagctgga cttcagccag gttgaaaaag cctaacccag cgatcaaaaa agcggcgatt
     1261 taatcgttgc acaaggcgcg agattggaat acaatttcgc gccttttgtt tttatgggcc
     1321 ttgcccgtaa aaacgatttt ttatatcacg gggagcctct cagaggcgtt attacccaac
     1381 ttgaggaatt tataatggct aagaaagtac aagcctatgt caagctgcag gttgcagctg
     1441 gtatggctaa cccgagtccg ccagtaggtc cggctctggg tcagcagggc gtaaacatca
     1501 tggaattctg caaagcgttc aacgcaaaaa ctgattccat cgaaaaaggt ctgccgattc
     1561 cggtagtaat caccgtttac gctgaccgtt ctttcacttt cgttaccaag accccgccgg
     1621 cagcagttct gctgaaaaaa gcggctggta tcaagtctgg ttccggtaag ccgaacaaag
     1681 acaaagtggg taaaatttcc cgcgctcagc tgcaggaaat cgcgcagacc aaagctgccg
     1741 acatgactgg tgccgacatt gaagcgatga ctcgctccat cgaaggtact gcacgttcca
     1801 tgggcctggt agtggaggac taagaaatgg ctaaactgac caagcgcatg cgtgttatcc
     1861 gcgagaaagt tgatgcaacc aaacagtacg acatcaacga agctatcgca ctgctgaaag
     1921 agctggcgac tgctaaattc gtagaaagcg tggacgtagc tgttaacctc ggcatcgacg
     1981 ctcgtaaatc tgaccagaac gtacgtggtg caactgtact gccgcacggt actggccgtt
     2041 ccgttcgcgt agccgtattt acccaaggtg caaacgctga agctgctaaa gctgcaggcg
     2101 cagaactggt aggtatggaa gatctggctg accagatcaa gaaaggcgaa atgaactttg
     2161 acgttgttat tgcttctccg gatgcaatgc gcgttgttgg ccagctgggc caggttctgg
     2221 gtccgcgcgg cctgatgcca aacccgaaag tgggtactgt aacaccgaac gttgctgaag
     2281 cggttaaaaa cgctaaagct ggccaggttc gttaccgtaa cgacaaaaac ggcatcatcc
     2341 acaccaccat cggtaaagtg gactttgacg ctgacaaact gaaagaaaac ctggaagctc
     2401 tgctggttgc gctgaaaaaa gcaaaaccga ctcaggcgaa aggcgtgtac atcaagaaag
     2461 ttagcatctc caccaccatg ggtgcaggtg ttgcagttga ccaggctggc ctgagcgctt
     2521 ctgtaaacta atgcctttac gtgggcggtg attttgtcta caatcttacc cccacgtata
     2581 atgcttaatg cagacgtata tccgagatat tcgggttgtg gcaaggcggc aactgagtga
     2641 gtcgccagga gcatagctaa ctatgtgact ggtgcgaatg aaggaagcca acgccgccac
     2701 aagttgaata gcgacggata gaaaagattt gttcgttgga gcctggccta tccaggcctc
     2761 cgtcgaagac cgcaggagtt tcgcaagaaa cttaatcccc tgcgtagacg gtgacagaac
     2821 gctaagatta ttcttttata ttctggcttg tttctgctca ccgtaattaa gacgctctct
     2881 ccgtttggag gagtgaagtg agttccagag attttctctg gcaaacatcc aggagcaaag
     2941 ctaatggctt taaatcttca agacaaacaa gcgattgttg ctgaagtcag cgaagtagcc
     3001 aaaggcgcgc tgtctgcagt agttgcggat tcccgtggcg taactgtaga taaaatgact
     3061 gaactgcgta aagcaggtcg cgaagctggc gtatacatgc gtgttgttcg taacactctg
     3121 ctgcgccgtg ctgttgaagg tactccgttc gagtgcctga aagacgcgtt tgttggtccg
     3181 accctgattg catactctat ggaacacccg ggcgctgctg ctcgtctgtt caaagagttc
     3241 gcgaaagcga atgcaaaatt tgaggtcaaa gccgctgcct ttgaaggtga gctgatcccg
     3301 gcgtctcaga tcgaccgcct ggcaactctg ccgacctacg aagaagcaat tgcacgcctg
     3361 atggcaacca tgaaagaagc ttcggctggc aaactggttc gtactctggc tgctgtacgc
     3421 gatgcgaaag aagctgctta atcgcagtta tctttttaac gcattcgctt acgtataaac
     3481 ttattctgat attcaggaac aatttaaatg tctatcacta aagatcaaat cattgaagca
     3541 gttgcagcta tgtctgtaat ggacgttgta gaactgatct ctgcaatgga agaaaaattc
     3601 ggtgtttccg ctgctgctgc tgtagctgta gctgctggcc cggttgaagc tgctgaagaa
     3661 aaaactgaat tcgacgtaat tctgaaagct gctggcgcta acaaagttgc tgttatcaaa
     3721 gcagtacgtg gcgcaactgg cctgggtctg aaagaagcta aagacctggt agaatctgca
     3781 ccggctgctc tgaaagaagg cgtgagcaaa gacgacgcag aagcactgaa aaaagctctg
     3841 gaagaagctg gcgctgaagt tgaagttaaa taagccaacc cttccggttg cagcctgaga
     3901 aatcaggctg atggctggtg actttttagt caccagcctt tttgcgctgt aaggcgccag
     3961 tagcgtttca cactgtttga ctactgctgt gcctttcaat gcttgtttct atcgacgact
     4021 taatatactg cgacaggacg tccgttctgt gtaaatcgca atgaaatggt ttaagcgtga
     4081 tagcaacagg cattgcggaa agtgttccat tttccggtca acaaaatagt gttgcacaaa
     4141 ctgtccgctc aatggacaga tgggtcgact tgtcagcgag ctgaggaacc ctatggttta
     4201 ctcctatacc gagaaaaaac gtattcgtaa ggattttggt aaacgtccac aagttctgga
     4261 tgtaccttat ctcctttcta tccagcttga ctcgtttcag aaatttatcg agcaagatcc
     4321 tgaagggcag tatggtctgg aagcagcttt ccgttccgta ttcccgattc agagctacag
     4381 cggtaattcc gagctgcaat acgtcagcta ccgccttggc gaaccggtgt ttgacgtcca
     4441 ggaatgtcaa atccgtggcg tgacctattc cgcaccgctg cgcgttaaac tgcgtctggt
     4501 gatctatgag cgcgaagcgc cggaaggcac cgtaaaagac attaaagaac aagaagtcta
     4561 catgggcgaa attccgctca tgacagacaa cggtaccttt gttatcaacg gtactgagcg
     4621 tgttatcgtt tcccagctgc accgtagtcc gggcgtcttc tttgactccg acaaaggtaa
     4681 aacccactct tcgggtaaag tgctgtataa cgcgcgcatc atcccttacc gtggttcctg
     4741 gctggacttc gaattcgatc cgaaggacaa cctgttcgta cgtatcgacc gtcgccgtaa
     4801 actgcctgcg accatcattc tgcgcgccct gaactacacc acagagcaga tcctcgacct
     4861 gttctttgaa aaagttatct ttgaaatccg tgataacaag ctgcagatgg aactggtgcc
     4921 ggaacgcctg cgtggtgaaa ccgcatcttt tgacatcgaa gctaacggta aagtgtacgt
     4981 agaaaaaggc cgccgtatca ctgcgcgcca cattcgccag ctggaaaaag acgacgtcaa
     5041 acttatcgaa gtcccggttg agtacatcgc aggtaaagtg gttgctaaag actatattga
     5101 tgagtctacc ggcgagctga tctgcgcagc gaacatggag ctgagcctgg atctgctggc
     5161 taagctgagc cagtctggtc acaagcgtat cgaaacgctg ttcaccaacg atctggatca
     5221 cggcccatat atctctgaaa ccttacgtgt cgacccaact aacgaccgtc tgagcgcact
     5281 ggtagaaatc taccgcatga tgcgccctgg cgagccgccg actcgtgaag cagctgaaag
     5341 cctgttcgag aacctgttct tctccgaaga ccgttatgac ttgtctgcgg ttggtcgtat
     5401 gaagttcaac cgttctctgc tgcgcgaaga aatcgaaggt tccggtatcc tgagcaaaga
     5461 cgacatcatt gatgttatga aaaagctcat cgatatccgt aacggtaaag gcgaagtcga
     5521 tgatatcgac cacctcggca accgtcgtat ccgttccgtt ggcgaaatgg cggaaaacca
     5581 gttccgcgtt ggcctggtac gtgtagagcg tgcggtgaaa gagcgtctgt ctctgggcga
     5641 tctggatacc ctgatgcctc aggatatgat caacgccaag ccgatttccg cagcagtgaa
     5701 agagttcttc ggttccagcc agctgtctca gtttatggac cagaacaacc cgctgtctga
     5761 gattacgcac aaacgtcgta tctccgcact cggcccaggc ggtctgaccc gtgaacgtgc
     5821 aggcttcgaa gttcgagacg tacacccgac tcactacggt cgcgtatgtc caatcgaaac
     5881 ccctgaaggt ccgaacatcg gtctgatcaa ctctctgtcc gtgtacgcac agactaacga
     5941 atacggcttc cttgagactc cgtatcgtaa agtgaccgac ggtgttgtaa ctgacgaaat
     6001 tcactacctg tctgctatcg aagaaggcaa ctacgttatc gcccaggcga actccaacct
     6061 ggatgaagaa ggccacttcg tagaagacct ggtaacttgc cgtagcaaag gcgaatccag
     6121 cttgttcagc cgcgaccagg ttgactacat ggacgtatcc acccagcagg tggtatccgt
     6181 cggtgcgtcc ctgatcccgt tcctggaaca cgatgacgcc aaccgtgcat tgatgggtgc
     6241 gaacatgcaa cgtcaggccg ttccgactct gcgcgctgat aagccgctgg ttggtactgg
     6301 tatggaacgt gctgttgccg ttgactccgg tgtaactgcg gtagctaaac gtggtggtgt
     6361 cgttcagtac gtggatgctt cccgtatcgt tatcaaagtt aacgaagacg agatgtatcc
     6421 gggtgaagca ggtatcgaca tctacaacct gaccaaatac acccgttcta accagaacac
     6481 ctgtatcaac cagatgccgt gtgtgtctct gggtgaaccg gttgaacgtg gcgacgtgct
     6541 ggcagacggt ccgtccaccg acctcggtga actggcgctt ggtcagaaca tgcgcgtagc
     6601 gttcatgccg tggaatggtt acaacttcga agactccatc ctcgtatccg agcgtgttgt
     6661 tcaggaagac cgtttcacca ccatccacat tcaggaactg gcgtgtgtgt cccgtgacac
     6721 caagctgggg ccggaagaga tcaccgctga catcccgaac gtgggtgaag ctgcgctctc
     6781 caaactagat gaatccggta tcgtttacat tggtgcggaa gtgaccggtg gcgacattct
     6841 ggttggtaag gtaacgccga aaggtgaaac tcagctgacc ccagaagaaa aactgctgcg
     6901 tgcgatcttc ggtgagaaag cctctgacgt taaagactct tctctgcgcg taccaaacgg
     6961 tgtatccggt acggttatcg acgttcaggt ctttactcgc gatggcgtag aaaaagacaa
     7021 acgtgcgctg gaaatcgaag aaatgcagct caaacaggcg aagaaagacc tgtctgaaga
     7081 actgcagatc ctcgaagcgg gtctgttcag ccgtatccgt gctgtgctgg tagccggtgg
     7141 cgttgaagct gagaagctcg acaaactgcc gcgcgatcgc tggctggagc tgggcctgac
     7201 agacgaagag aaacaaaatc agctggaaca gctggctgag cagtatgacg aactgaaaca
     7261 cgagttcgag aagaaactcg aagcgaaacg ccgcaaaatc acccagggcg acgatctggc
     7321 accgggcgtg ctgaagattg ttaaggtata tctggcggtt aaacgccgta tccagcctgg
     7381 tgacaagatg gcaggtcgtc acggtaacaa gggtgtaatt tctaagatca acccgatcga
     7441 agatatgcct tacgatgaaa acggtacgcc ggtagacatc gtactgaacc cgctgggcgt
     7501 accgtctcgt atgaacatcg gtcagatcct cgaaacccac ctgggtatgg ctgcgaaagg
     7561 tatcggcgac aagatcaacg ccatgctgaa acagcagcaa gaagtcgcga aactgcgcga
     7621 attcatccag cgtgcgtacg atctgggcgc tgacgttcgt cagaaagttg acctgagtac
     7681 cttcagcgat gaagaagtta tgcgtctggc tgaaaacctg cgcaaaggta tgccaatcgc
     7741 aacgccggtg ttcgacggtg cgaaagaagc agaaattaaa gagctgctga aacttggcga
     7801 cctgccgact tccggtcaga tccgcctgta cgatggtcgc actggtgaac agttcgagcg
     7861 tccggtaacc gttggttaca tgtacatgct gaaactgaac cacctggtcg acgacaagat
     7921 gcacgcgcgt tccaccggtt cttacagcct ggttactcag cagccgctgg gtggtaaggc
     7981 acagttcggt ggtcagcgtt tcggggagat ggaagtgtgg gcgctggaag catacggcgc
     8041 agcatacacc ctgcaggaaa tgctcaccgt taagtctgat gacgtgaacg gtcgtaccaa
     8101 gatgtataaa aacatcgtgg acggcaacca tcagatggag ccgggcatgc cagaatcctt
     8161 caacgtattg ttgaaagaga ttcgttcgct gggtatcaac atcgaactgg aagacgagta
     8221 attctcgctc aaacaggtca ctgctgtcgg gttaaaaccc ggcagcggat tgtgctaact
     8281 ccgacgggag caaatccgtg aaagatttat taaagtttct gaaagcgcag actaaaaccg
     8341 aagagtttga tgcgatcaaa attgctctgg cttcgccaga catgatccgt tcatggtctt
     8401 tcggtgaagt taaaaagccg gaaaccatca actaccgtac gttcaaacca gaacgtgacg
     8461 gccttttctg cgcccgtatc tttgggccgg taaaagatta cgagtgcctg tgcggtaagt
     8521 acaagcgcct gaaacaccgt ggcgtcatct gtgagaagtg cggcgttgaa gtgacccaga
     8581 ctaaagtacg ccgtgagcgt atgggccaca tcgaactggc ttccccgact gcgcacatct
     8641 ggttcctgaa atcgctgccg tcccgtatcg gtctgctgct cgatatgccg ctgcgcgata
     8701 tcgaacgcgt actgtacttt gaatcctatg tggttatcga aggcggtatg accaacctgg
     8761 aacgtcagca gatcctgact gaagagcagt atctggacgc gctggaagag ttcggtgacg
     8821 aattcgacgc gaagatgggt gcggaagcaa tccaggctct gctgaagagc atggatctgg
     8881 agcaagagtg cgaacagctg cgtgaagagc tgaacgaaac caactccgaa accaagcgta
     8941 aaaagctgac caagcgtatc aaactgctgg aagcgttcgt tcagtctggt aacaaaccag
     9001 agtggatgat cctgaccgtt ctgccggtac tgccgccaga tctgcgtccg ctggttccgc
     9061 tggatggtgg tcgtttcgcg acttctgacc tgaacgatct gtatcgtcgc gtcattaacc
     9121 gtaacaaccg tctgaaacgt ctgctggatc tggctgcgcc ggacatcatc gtacgtaacg
     9181 aaaaacgtat gctgcaggaa gcggtagacg ccctgctgga taacggtcgt cgcggtcgtg
     9241 cgatcaccgg ttctaacaag cgtcctctga aatctttggc cgacatgatc aaaggtaaac
     9301 agggtcgttt ccgtcagaac ctgctcggta agcgtgttga ctactccggt cgttctgtaa
     9361 tcaccgtagg tccatacctg cgtctgcatc agtgcggtct gccgaagaaa atggcgctgg
     9421 agctgttcaa accgttcatc tacggcaagc tggaactgcg tggtcttgct accaccatta
     9481 aagctgcgaa gaaaatggtt gagcgcgaag aagctgtcgt ttgggatatc ctggacgaag
     9541 ttatccgcga acacccggta ctgctgaacc gtgcaccgac tctgcaccgt ctgggtatcc
     9601 aggcatttga accggtactg atcgaaggta aagctatcca gctgcacccg ctggtttgtg
     9661 cggcatataa cgccgacttc gatggtgacc agatggctgt tcacgtaccg ctgacgctgg
     9721 aagcccagct ggaagcgcgt gcgctgatga tgtctaccaa caacatcctg tccccggcga
     9781 acggcgaacc aatcatcgtt ccgtctcagg acgttgttct gggtctgtac tatatgaccc
     9841 gtgactgtgt taacgccaaa ggcgaaggca tggtgctgac tggcccgaaa gaagcagaac
     9901 gtctgtatcg ctctggtctg gcttctctgc atgcgcgcgt taaagtgcgt atcaccgagt
     9961 atgaaaaaga tgcaaacggt gaattagtag cgaaaaccag cctgaaagac acgactgttg
    10021 gccgtgccat tctgtggatg attgtaccga aaggtctgcc ttactccatc gtcaaccagg
    10081 cgctgggtaa aaaagcaatc tccaaaatgc tgaacacctg ctaccgcatt ctcggtctga
    10141 aaccgaccgt tatttttgcg gaccagatca tgtacaccgg cttcgcctat gcagcgcgtt
    10201 ctggtgcatc tgttggcatc gatgacatgg tcatcccgga gaagaaacac gaaatcatct
    10261 ccgaggcaga agcagaagtt gctgaaattc aggagcagtt ccagtctggt ctggtaactg
    10321 cgggcgaacg ctacaacaaa gttatcgata tctgggctgc ggcgaacgat cgtgtatcca
    10381 aagcgatgat ggataacctg caaactgaaa ccgtgattaa ccgtgacggt caggaagaga
    10441 agcaggtttc cttcaacagc atctacatga tggccgactc cggtgcgcgt ggttctgcgg
    10501 cacagattcg tcagcttgct ggtatgcgtg gtctgatggc gaagccggat ggctccatca
    10561 tcgaaacgcc aatcaccgcg aacttccgtg aaggtctgaa cgtactccag tacttcatct
    10621 ccacccacgg tgctcgtaaa ggtctggcgg ataccgcact gaaaactgca aactccggtt
    10681 acctgactcg tcgtctggtt gacgtggcgc aggacctggt ggttaccgaa gacgattgtg
    10741 gtacccatga aggtatcatg atgactccgg ttatcgaagg tggtgatgtt aaagagccgc
    10801 tgcgcgatcg cgtactgggt cgtgtaactg ctgaagacgt tctgaagccg ggtactgctg
    10861 atatcctcgt tccgcgcaac acgctgctgc acgaacagtg gtgtgacctg ctggaagaga
    10921 actctgtcga cgcggttaaa gtacgttctg ttgtatcttg tgacaccgac tttggtgtat
    10981 gtgcgcactg ctacggtcgt gacctggcgc gtggccacat catcaacaag ggtgaagcaa
    11041 tcggtgttat cgcggcacag tccatcggtg aaccgggtac acagctgacc atgcgtacgt
    11101 tccacatcgg tggtgcggca tctcgtgcgg ctgctgaatc cagcatccag gtgaaaaaca
    11161 aaggtagcat caagctcagc aacgtgaagt cggttgtgaa ctccagcggt aaactggtta
    11221 tcacttcccg taatactgaa ctgaaactga tcgacgaatt cggtcgtacc aaagaaagct
    11281 acaaagtacc ttacggtgcg gtactggcga aaggcgatgg cgaacaggtt gctggcggcg
    11341 aaaccgttgc aaactgggac ccgcacacca tgccggttat caccgaagta agcggttttg
    11401 tacgctttac tgacatgatc gacggtcaga ccattactcg tcagacagac gaattgaccg
    11461 gtctgtcttc gctggtggtt ctggattccg cagaacgtac cgcaggtggt aaagatctgc
    11521 gtccggcact gaaaatcgtt gatgctcagg gtaacgacgt tctgattccg ggtaccgata
    11581 tgcctgcgca gtacttcctg ccgggtaaag cgattgttca gctggaagat ggcgtacaga
    11641 tcagctctgg tgacaccctg gcgcgtattc cgcaggaatc cggcggtacc aaggacatca
    11701 ccggtggtct gccgcgcgtt gcggacctgt tcgaagcacg tcgtccgaaa gagccggcaa
    11761 tcctggctga aatcagcggt atcgtttcct tcggtaaaga aaccaaaggt aaacgtcgtc
    11821 ttgttatcac cccggtagac ggtagcgatc cgtacgaaga gatgattccg aaatggcgtc
    11881 agctcaacgt gttcgaaggt gaacgtgtag aacgtggtga cgtaatttcc gacggtccgg
    11941 aagcgccgca cgacattctg cgtctgcgtg gtgttcatgc tgttactcgt tacatcgtta
    12001 acgaagtaca ggacgtatac cgtctgcagg gcgttaagat taacgataaa cacatcgaag
    12061 ttatcgttcg tcagatgctg cgtaaagcta ccatcgttaa cgcgggcagc tccgacttcc
    12121 tggaaggcga acaggttgaa tactctcgcg tcaagatcgc aaaccgcgaa ctggaagcga
    12181 acggcaaagt gggtgcaact tactcccgcg atctgctggg tatcaccaaa gcgtctctgg
    12241 caaccgagtc cttcatctcc gcggcatcgt tccaggagac cactcgcgtg ctgaccgaag
    12301 cagccgttgc gggcaaacgc gacgaactgc gcggcctgaa agagaatgtt atcgtgggtc
    12361 gtctgatccc ggcaggtacc ggttacgcgt accaccagga tcgtatgcgt cgccgtgctg
    12421 cgggtgaagc tccggctgca ccgcaggtga ctgcagaaga cgcatctgcc agcctggcag
    12481 aactgctgaa cgcaggtctg ggcggttctg ataacgagta atcgttaatc cgcaaataac
    12541 gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga gcatttgtca
    12601 gaatatttaa ggaatttctg aatactcata atcaatgtag agatgactaa tatcctgaaa
    12661 ctgactgaac taattgagtc aaactcggca aggattcgat actattcctg tgtaactttc
    12721 ttaaggaacg agaatgaaac aggaagtgga aaagtggcga ccttttggag atccggatgg
    12781 tgatattcgt gatttatcat ttcttgatgc tcatcaggct gtctacgttc agcatcatga
    12841 gggcaaagag cctttagagt atcgcttttg ggttacctac tctcttcact gcttcacaaa
    12901 agattatgaa catcagacga acgaagaaaa acaatcgtta atgtaccacg cgcctaaaga
    12961 atctcgtccc ttctgccagc accgttataa cttagcgcgc acacacttaa aaagaactat
    13021 tttggcgctg ccagaaagca acgttattca tgccgggtat ggtagctatg ccgtgattga
    13081 ggtggactta gacggaggag ataaggcatt ttactttgtt gcgttcaggg ctttcaggga
    13141 aaagaaaaaa ctccgtttgc atgtaactag cgcttatccc atttctgaaa aacagaaagg
    13201 taaatcagtg aaatttttca ccattgccta caacttattg agaaataagc agcttcctca
    13261 gccctcaaaa taacaaaacc caccttaagg tgggtttcgc cagagaatta tctctggtat
    13321 tcagaacgcc attaccggac tttgccttga ccttgcgata atcgcaggtt gcgggatgtc
    13381 tgaatttctt cagtctgctg catcctggaa gatgagaaca tgtgttctta ttttcgtctc
    13441 tatcatagtt gagtatttac tctcttacaa tcagatctct ttcattgctc aacaggcgat
    13501 ggcttcagac tttgcattac ggaattttta agaaaggcag ggcgaaacga ggaagaagct
    13561 tttcgccctg gacaacattc ctgctgacgg cactaccata aaaaaacaca acgctttcaa
    13621 caaccttgcg tttttcatct tccgtagcgg ctcggaataa atttacgttc ttacattccg
    13681 tctcatagtc tttgcgaggc ccgtcccaga tagctgtccc agtctttcca taccggctgc
    13741 aaaccctgag cggttaacgc ggcagcaacc gcttccggtc tgcgatcgtc gtgcggtgag
    13801 aactgttcca actcggggtg attatcggca tagccacctg gctgcgtttt cgagaaggcg
    13861 ctgacgttat tgatcgccag cggaataacg cgatcgcgaa accacggtga ttcccgcgtg
    13921 gagagtgaca gttcaatctc cggtgcaagc agtcggaagg cgcagatggt ttgcactaac
    13981 tggcgttcat ccataatcga cgcaggctca atgccgccag tacacgggcg caggcgcgga
    14041 aaggagacag agtaacggct ttgccagtaa tgctgttgca gccatagcaa atgttcggca
    14101 accatatagc agtcaacgcg tcagttgtcg gaaaggccaa ttagcgcgcc gaggcctatc
    14161 ttatcaatcc ccgcacgccc cagccgatcc ggcgtttcca gccgccagaa gaagtcctgt
    14221 tttttgcctt tcagatgatg gcgggcataa gtcgcctcgt gatatgtctc ctgataaacc
    14281 atcacgccat ccagaccaag ctgctttaac tcggcgtatt ccgtcttcgc cagcggttgc
    14341 acttccattt gtagtgaaga gaactgttca cgcagggcgg ggagatgacg acgaaagtaa
    14401 tccatcccca ctttcgcctg atgttcacca gtgactaata gcagatgttc aaagcccatc
    14461 tcccgtatag cggcgctttc cctggcaata tccgcttcat ccagcgtttt gcgcttgatg
    14521 cgattactca tggaaaatcc gcagtacgtg cagtcgttag cgcaaagatt ggaaagataa
    14581 agcgggacgt agaaactaac tgtgttgcca aatcgctgac gggtcagacg ctgcgcccgt
    14641 tgggccagtt gttccagata gccactggcg gctggcgata acagcgccat catatcgtcg
    14701 cgggtgaatt gcgaggcatt tagcgcccgc tctacgtcaa cagccgtttt gccgttgata
    14761 cgcaggcgga tgtcatccca gtccagttgt cgccagcgat cgctgaaggt tttcatgccg
    14821 atgcctccag aaatccagtc agcgggctgg tggcatgagc aaaatgactg cggctgcccg
    14881 gtccggactg acgtgccagc aggcctgctt ctaccgccag acgaaatgcc ttcgccatgt
    14941 tgacgggatc gtccgctacg gcaatcgccg tattcactaa caccgcgtcg gcccccattt
    15001 ccagggcctg cgcggcatgg ctgggaacgc cgatgccagc atcgacaacc accggaactg
    15061 tggcctgctg gataataatc tccagcatgg cacgggtttc cagtccctga ttcgagccaa
    15121 tcggcgcgcc gagcggcatc accgctgcac agccgacttc ttccagacgt ttacacaata
    15181 ccggatcggc cccgcagtaa ggcagcacga caaatccctg ttgtaccagc atttcggcgg
    15241 ctttcagagt ttcgatggga tcgggcaaca gccagcgggc gtcagggtga atctctaatt
    15301 ttaaccagtt tgtgcctaac gcttcacgtg ccagatgggc ggcgaaaatg gcttcttccg
    15361 ctgttttcgc cccggaagta tttggcagca gggtcacacc cgccgcgata agcggttcga
    15421 ggatagcgtc gttgtgctgg cgcaagtcga cacgtttcat cgccagtgtc accagctggc
    15481 tgccggaagc gcggatcgcc tccaccatca gttgtgaaga agcgaatttc cctgtgccgg
    15541 taaacagatg tgaatcaaac gttttgtccg caatacgtaa catctcaacc ccctgcaata
    15601 acctgaaaaa gcaggatctg gtcgccatcc tgcacgatat gttgcgtcca ctgctcacgc
    15661 gggacgattt gctgattaat cgccagagcc gcgcccgctt gtcgttggtc gagttgctcc
    15721 agtagttcgt gaacagtttg cccggcggcg cactgcatcg gttgatcgtt aaacaggatc
    15781 tgcattgctt cctccgcata ccgggcaacc actggcgcgg cgcaacgcca ggctgcgcca
    15841 ctggctcgat ttaccgtcga acagtcggag ttctcccgca ggtgtctcta taccgcttaa
    15901 taacttaatg gcttccagtg cctgcaaagt gcccataacc ccgaccaccg ggccaaccac
    15961 gcccgccgtg cggcagttgc gttctggctc ctggttatct ggccacaggc agcggtaaca
    16021 cccctgctcc cagggcggcg tcagtaccat caactgaccg ccaaatccga ccgcgctggc
    16081 ggtgataagc ggcgtgttga gtaccacgca ggcggcatta atctcctggc gagtcgccat
    16141 attgtcggta ccgtcgagca ccacatcggc ccgtgcaacc gcatctttta acgcctcacc
    16201 cgttaaccgt tgttgtaatg ctgtcagttg aatgtcggga ttcaactgtg tcagtcgctg
    16261 ttggctgacc tgtgatttcg ggctgttgat atcttcagtg gtaaagagaa tttgtcgtta
    16321 cagattgctt aaatgcacat cgtcgtcatc tgccagtacc agcgtcccga cgccagcgcc
    16381 cgccaggtat agcgcagcag gtgtacccag cccgccaaga ccgataatca gcacctggct
    16441 gtcgagcagt ttttgctgcc cgtcaagagc gatatcgtcg agcaggattt ggcggctata
    16501 acgcataaaa tcacggtcat tcatcgccaa ctcctgcaat ttccagcaac tgtgccgttg
    16561 ccaaacgcca gtctgcggct tgagtaatgg cgctgacgac ggcgatactg ccgacacccg
    16621 ttgctatcac cgcaggcgcg cgtgccagac tgataccgcc aatcgccacg gtgggataat
    16681 ccgccagtcg ctcaacatgc cgtgccagct gttccagccc ctgcggtgca gaaggcatct
    16741 gtttggtttg cgtcgggaac acatgtccca gcgcgatata agaggggcgt gctgccagcg
    16801 cgacgtcgat ttccatatcg tcatgtgtcg aaacacccag ccgcaggcct gccgcgcgga
    16861 tggcattgag atcggtggct tgcaaatctt cctgccccaa atggacgcca tacgcctgat
    16921 gcttgatcgc gagccgccag taatcgttga taaacaatcg cgcgttatag cggcggccca
    16981 gcgcaattgc cgccacgaca tcggcttcca cctcttcatc gcgccgatct ttgatgcgta
    17041 gctggagagt acgtacgcct gcatccaaca gacgttcgat ccactgtacg ctgtccacca
    17101 ctgggtacag tcctaaacga aaaggtacag gaggaaaatc aggctgatac atcacgcttc
    17161 ctccttacgc aggtagattt ctccgcctct ggcacggaag ttctccgaca tatccgccat
    17221 tcccatttca atagtttgcg tggcggcgta atcacgcact tcctggctga tttttatcga
    17281 gcagaatttc ggcccacaca tggagcaaaa atgggcgact ttacctgact cttgcggcag
    17341 ggtttcatcg tgataagcgc gggcggtaaa cgggtcgagg gccagattaa actggtcttc
    17401 ccagcgaaat tcgaagcggg ctttcgacat ggcgttatcg cgaatttgcg cgcccggatg
    17461 ccctttcgcc aggtcagcgg cgtgggcggc aatcttatag gtgataagcc cctgcttaac
    17521 atcttcttta ttaggcagac ccagatgctc ttttggcgtt acgtaacaga gcatcgcgca
    17581 gccaaaccag ccaatcatcg ccgcaccaat ccccgacgtg aagtggtcat agcccggcgc
    17641 aatatcggta gttagcggcc ccagagtgta aaacggcgct tcgtggcagt gctctaactc
    17701 ctcggtcata ttgcggcgga tcatctgcat cggcacgtgg cctgggcctt caatcatcac
    17761 ctgcacgtca tattcccagg caattttggt cagttcgccc agcgtatgca gctcggcaaa
    17821 ctgcgcttca tcgttggcgt cctgaataga accggggcgc agaccgtcgc ccagcgacag
    17881 cgaaacgtca taagcggcac agatttcaca aatttcgcgg aagtgttgat agaggaaatt
    17941 ttcctgatga tgggagaggc accatttcgc cataatcgaa ccgccgcgag agacgatacc
    18001 ggtcaggcgt ttcgcggtca tcggcacata gcgcaacagt acgcccgcat ggatagtgaa
    18061 gtaatccaca ccttgctcgg cctgttccag cagcgtgtcg cggaacgctt cccaggtaag
    18121 atcttcggcg atcccgttaa ccttctccag cgcctggtag atcggcactg taccgatcgg
    18181 caccggactg ttacgcaaaa tccactcgcg ggtttcgtga atatagcgac cggtggagag
    18241 atccatcacc gtatccgctc cccagcgcgt ggaccatacc agcttttcca cttcttcttc
    18301 gatggaagag gtgaccgccg agttgccgat attggcgtta acttttacca ggaagttacg
    18361 gccaataatc atcggctccg attccggatg attaatgttg gccgggataa tcgcacgtcc
    18421 ggcagcaact tcatcacgga caaattccgc agtgatattt tccggcagac gtgcgccaaa
    18481 gctcattccc ggatgctggt ggcgtaaaac ttcgctacgt atgcgctcgc ggcccatatt
    18541 ctcgcggatg gcgatgaatt ccatttccgg tgtgatgatg ccctggcggg cgtagtgcag
    18601 ttgggtgaca cggcgtcctg ctttggcgcg ttttggtgtt agtacgccgc taaaacgcag
    18661 ttcgtcgagg ccatcatctg ccagccgcgc tttagtgtaa tcggaactgc gcacggtaag
    18721 ttcttcggta tcgccgcgcg catcgatcca cggctggcgt agttttgcca gcccttgctg
    18781 cacgttaatg gcgatctgcg gatcgccata tgggccggag gtgtcgtaga ccggaatcgc
    18841 ttcgttttct tcaaactgca gctgttcttt gctgccgccg attagcgtcg ggctaagctg
    18901 gatctcacgc atcggcacgc gaacgccaga gtgtgtgcca gtgaggtaaa tgcgttttga
    18961 gttgggaaag gcggtgcctt ccagggtgtc gataaaatgt tgggcccggg cgcgttgttc
    19021 gcggcgggtc agttttgttg cagacatagc tcattccaaa aagttaagga cgtggcttgt
    19081 cagacgacgg atgaagcaag agacgatcgc cgcaaaggcg atgcgatagc agattaactc
    19141 ttgttccctt cgcaggtatt agcctgatca ggttccgcgg atcccgaata aacggtctca
    19201 gccagttaag gcactccgac aagaaattag cccccacaaa cggggcgttg aatgtaaatt
    19261 acgcgttaac agcgcagaac tcaagcagga tgtttgacgc gggcggcgtc aagcaccagc
    19321 agaatcagct tatcttccag cacaaagcgc gcttccagcg cctcgccgat gtcagataaa
    19381 acctgttgaa actcaaggta attatcatga tcgatggcgg tttccaggct ggaatcgtag
    19441 taatccataa tctgttgggt attggcttcg agttgcggcc aaatcttcgc ggcgcgcgcg
    19501 agttgcccgt tgccttccag cttatgaaga atgcgctcat aaatactgaa atgtccggca
    19561 gacaagtaat cgaccaggct ctgacaaaaa tcatcaaggg ctttttcgtt tagcctcatg
    19621 tacgattctt tgccaggctt aatgccaacc agattgtagt aagccacgag cagatgctta
    19681 cgtacatgta gccagcgatc aaccagtttg ttacttcctc tgacgcgctc cgtcaggtta
    19741 tcgagctggt taagcatgat tgactccgca agtttgtatt caaaaactgc tcagtgagaa
    19801 atgtaaaaac catgttaaac atgccagtga tgcaaaggta gtgcaagagc tatggatcgt
    19861 ataattgaaa aattagatca cggctggtgg gtcgtcagcc atgaacaaaa attatggttg
    19921 ccgaagggag aattgccata tggcgaagcg gcaaatttcg atcttgtggg tcagcgcgca
    19981 ctacaaatcg gcgaatggca gggggaacct gtttggttag tacaacagca gcggcgtcac
    20041 gatatggggt cggtacgtca ggtcattgat ctcgatgttg tgctgtttca actggccgga
    20101 cgaggcgtac aactggcgga gttttaccga tcgcataaat actgtggtta ctgcgggcat
    20161 gaaatgtatc cgagcaaaac cgaatgggcg atgctgtgca gccattgccg tgagcgttac
    20221 tacccgcaaa tcgccccctg cattattgtt gccatccgtc gcgatgattc gatcctcctc
    20281 gcccagcata cccgccatcg taacggtgtc catacagtac ttgccggatt cgtcgaagtg
    20341 ggcgaaaccc tcgagcaggc agtcgcgcgg gaagtgatgg aagagagcgg aattaaagtt
    20401 aaaaacttgc gttacgtgac ttctcagccg tggccgtttc ctcagtcttt aatgaccgcg
    20461 tttatggcgg aatatgacag cggcgacatc gtgatcgacc cgaaagaatt gctcgaggcg
    20521 aactggtatc gctatgacga tttgccgtta ctcccgccgc ccggcaccgt agcgcgccgt
    20581 ctgatagaag atactgtggc gatgtgtcgg gcagagtatg agtgatgata cactgaccgc
    20641 ctgacgcact aaggaacagc caaaatgacc gaacttaaaa acgatcgtta tctgcgggcg
    20701 ctgctgcgcc agcccgttga tgtcactcca gtatggatga tgcgccaggc gggtcgctat
    20761 ctaccggaat ataaagccac gcgcgcccag gcgggcgatt ttatgtcgct gtgcaaaaac
    20821 gccgagctgg cgtgcgaagt gactttgcaa ccgctgcgtc gctacccgct ggatgcggcg
    20881 atcctctttt ccgatatcct caccgtgccg gacgcgatgg ggttagggct ctattttgaa
    20941 gccggagaag gtccgcgttt tacctcgcca gtcacctgca aagccgacgt cgataagctg
    21001 ccaattccgg acccggaaga tgagctgggt tacgtgatga acgcggtgcg taccattcgt
    21061 cgcgaactga aaggcgaagt gccgctgatt ggtttttccg gcagcccgtg gacgctggca
    21121 acctacatgg tggaaggcgg cagcagcaaa gccttcaccg tgatcaaaaa aatgatgtat
    21181 gccgatccgc aggcgctgca cgctctactc gataaactgg cgaaaagcgt cactttgtat
    21241 ctgaatgcgc agattaaagc cggtgctcag gcagtgatga ttttcgacac ctggggcggt
    21301 gtgcttaccg ggcgcgatta tcaacagttc tcgctctatt acatgcataa aattgttgat
    21361 ggtttactgc gtgaaaacga cggtcgccgc gtaccggtca cgctgtttac caaaggcggc
    21421 ggacagtggc tggaagcgat ggcagaaacc ggttgcgatg cgttgggcct cgactggaca
    21481 acggatatcg ccgatgcgcg ccgccgtgtg ggcaataaag tcgcgttgca gggtaatatg
    21541 gatccgtcga tgctgtacgc gccgcctgcc cgcattgaag aagaagtagc gactatactt
    21601 gcaggtttcg gtcacggcga aggtcatgtc tttaaccttg gtcacggcat tcatcaggat
    21661 gtgccgccag aacatgctgg cgtgttcgtg gaggcagtgc atcgactgtc tgaacaatat
    21721 caccgctaag gagtgattat ggatctcgcg tcattacgcg ctcaacaaat cgaactggct
    21781 tcttctgtga tccgcgagga tcgactcgat aaagatccac cggatctgat cgccggagcc
    21841 gatgtcgggt ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct gctgaaatat
    21901 ccctcgcttg agctggtgga gtataaagtt gcccgcatcg ccaccaccat gccttacatt
    21961 ccaggttttc tttccttccg cgaatatcct gcgctgctgg cagcgtggga gatgctgtcg
    22021 caaaagccgg atttagtgtt tgtcgatggt catgggatct cgcatcctcg ccgtcttggc
    22081 gtcgccagcc attttggttt gatggtggat gtgccaacga tcggcgtggc gaaaaaacgg
    22141 ctctgcggta aattcgaacc gctctccagc gaaccgggcg cgctggcccc actgatggat
    22201 aaaggcgagc agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc gttgtttatc
    22261 gctaccggcc atcgggtcag cgtggacagc gcgctggcgt gggtacaacg ctgcatgaaa
    22321 ggctatcgtc tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga acgtccggcg
    22381 ttcgtgcgct atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa tttccgtatt
    22441 tgagaactta acatgttaca aaacccaatt catctgcgtc tggagcgcct ggaaagctgg
    22501 cagcatgtca ctttcatggc ttgcttatgc gaacgcatgt accccaatta cgccatgttc
    22561 tgccagcaaa ccggttttgg tgatgggcaa atttaccgtc gtattctcga tctcatctgg
    22621 gaaacgctga ccgttaaaga cgcaaaagta aatttcgaca gccaactgga gaaatttgaa
    22681 gaagcgattc cttcagccga cgatttcgat ctgtacggcg tttatccggc aatcgatgcc
    22741 tgcgtggcgt taagtgaact ggtccattcg cgtttgagtg gtgaaacgct cgaacacgcg
    22801 gtggaagtga gtaagacctc catcacgacc gttgcgatgc tggaaatgac tcaggttggt
    22861 cgcgaaatga gcgatgaaga gcttaaagaa aacccagctg tagagcaaga atgggacatt
    22921 cagtgggaaa tattccgact tttagctgag tgcgaagaac gcgatatcga gctgataaaa
    22981 ggccttaggg cagacctgcg tgaggcgggt gagagcaata ttggtataat ttttcagcaa
    23041 taagaccaga aaacgtgatt taacgcctga tttgtcgtac ctggagtctt ccctttcgcc
    23101 ccccgtctgg tctacatttg gggggcgaaa aaaagtggct atcggtgcgt gtatgcagga
    23161 gagtgctatt ctggcatttc cgtcgcactc gatgcttagc aagcgataaa cacattgtaa
    23221 ggataactta tgaacaagac tcaactgatt gatgtaattg cagagaaagc agaactgtcc
    23281 aaaacccagg ctaaagctgc tctggagtcc actctggctg caattactga gtctctgaaa
    23341 gaaggcgatg ctgtacaact ggttggtttc ggtaccttca aagtgaacca ccgcgctgag
    23401 cgtactggcc gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc taacgtaccg
    23461 gcatttgttt ctggcaaggc actgaaagac gcagttaagt aagattgcgt ggcagtgaac
    23521 agttttaacg aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc attgatgctg
    23581 gctggcgctc tgcttctcac tgcctgtagt cataactctt cacttcctcc ctttaccgcc
    23641 agtggatttg ctgaagacca gggcgcggta cgcatctggc gaaaagacag cggcgataat
    23701 gtgcatctgc ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac gcgagagtat
    23761 cgctggcagg gcgataacct cacgctcatc aatatcaatg tttacagcaa accgccggtg
    23821 aatattcgcg cgcgttttga cgatcgcggt gatctgagct ttatgcaacg tgaatccgat
    23881 ggggaaaagc agcagctttc taacgaccaa atcgatttat accgttatcg tgctgatcag
    23941 atccgccaga ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca ggggcgctgg
    24001 catgcgatgg aacagaccgt gaccacctgc gaagggcaaa ccattaaacc tgatttagat
    24061 tcgcaggcga tagcgcatat cgagcgccgc cagagccgct cttctgttga tgtcagcgtg
    24121 gcatggctgg aagcgcccga aggttcgcaa ttactgttag tggcaaactc tgatttctgt
    24181 cgctggcaac ccaacgagaa aacgttctga tttaccagtg gcccataccc atatgaccgc
    24241 caccaccgca gccgccgtag cccatgccca ttccggctcc gcgcggaata cccgcttcag
    24301 ccatcgcgat atctcgtttc acccgtaact catctaacga ctgacgcaaa ttctccatct
    24361 ctttggcgac cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac agggcattgt
    24421 attcataacg cttcgtcacc agttgctgtt gcagtgcgct gctttgagcg taaaagtcat
    24481 tatggatttt ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca ttttgctgcc
    24541 acataccgtg tccgccgtga gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg
    24601 ccatcattac cagggcaatt ttcgtgttcc gtttcatggt taatcctcca gtggttgtct
    24661 tacttcgggt attgcatctt ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg
    24721 catgataaac gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt ttactcattg
    24781 aaacttaagc ctttgtgtta cagcgcaggg taagcgctga taaaagatgg catgatttct
    24841 gctgtcagaa agggatgagc aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga
    24901 ctccttagct aaatggttaa gcgcgatcct ccccgtggtc attgttgggc tggtagggtt
    24961 gtttgcggtg acggtgattc gcgattatgg gcgcgagact gccgccgcca gacaaacgct
    25021 gctggaaaaa ggcagtgtac ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg
    25081 gatgcgcatg catcatgcgc agcagcaggc cctactggaa gaaatggccg ggcagcctgg
    25141 agtacgttgg tttgcggtca cggatgaaca aggaacaatc gtgatgcata gcaactccgg
    25201 catggtggga aaacagcttt attccccgca ggaaatgcag cagttacatc cgggagatga
    25261 agaagcgtgg cggcggatcg atagcgcaga cggtgagcct gttctggaaa tttatcgcca
    25321 gtttcaaccg atgtttgctg ctggaatgca ccggatgcgc catatgcagc agtatgccgc
    25381 gacaccacaa gcaattttca tcgctttcga cgccagtaat attgtgagtg ccgaagatcg
    25441 tgagcagaga aacaccctga ttatcctctt cgccctggcg acggtcttgc tggcaagcgt
    25501 attgtcattc ttctggtatc gccgctatct gcgctcgcgc cagcttctac aagatgaaat
    25561 gaagcgcaaa gagaagctgg tggcactggg gcatctggcg gcaggcgttg cccacgaaat
    25621 ccgtaatcca ctttcctcaa ttaaagggct ggcgaaatac tttgccgaac gcgcgccagc
    25681 agggggagaa gcgcatcaac tggcgcaggt gatggcgaaa gaagccgacc gtttaaaccg
    25741 cgtggtaagc gagttgctgg aactggttaa gccaacgcat ctggctttgc aggcggtgga
    25801 tctcaacacg ctgattaacc actcattaca gctggtaagc caggatgcaa acagccggga
    25861 gatccagtta cgctttaccg ccaacgacac attaccggaa attcaggccg atccggacag
    25921 gctgactcag gtcctgttga atctctatct caatgctatt caggcgattg ttcagcatgg
    25981 cgtgattagc gtgacggtca gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag
    26041 cggtaaggga attgcggcag atcagcttga agccatcttc actccgtact tcaccaccaa
    26101 agccgaaggc accggattgg ggctggcggt cgtgcataat attgttgaac aacacggtgg
    26161 tacaattcag gtcgcaagcc aggagggaaa aggctcaacg ttcaccctct ggcttccggt
    26221 caatattacg cgtaaggacc cacaaggatg acgcacgata atatcgatat tctggtggtg
    26281 gatgatgaca ttagccactg cactattttg caggctttac tgcgcggctg gggctataac
    26341 gtcgcgctgg cgaacagcgg gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat
    26401 cttgtgcttt gcgatgtgcg aatggcggag atggacggca tcgccacgct gaaagagatc
    26461 aaagcgttaa acccggcaat tccggtgctg attatgactg cgtactccag cgtcgagacg
    26521 gcggtagagg cactgaaaac tggggcgctg gattatctca tcaagccgct ggatttcgat
    26581 aacctacagg cgacgctgga aaaggcgctc gcgcatacgc acagtattga tgctgaaaca
    26641 cctgcggtga ctgccagcca gttcggtatg gtcggtaaaa gcccggtgat gcaacacctg
    26701 ctcagtgaaa tcgccctcgt cgcgccatcg gaagccacag tgctgatcca cggcgattcc
    26761 ggcaccggta aagagctggt cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa
    26821 ccgctggtaa cgctcaactg tgcggcactc aacgaatcct tgctggaatc tgaattgttc
    26881 ggtcacgaaa aaggggcgtt tactggagcc gataaacgcc gggaagggcg ctttgttgag
    26941 gcggacggcg gcacgctgtt tctcgatgaa attggcgata tctcgccgat gatgcaggtg
    27001 cgtctgctgc gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc
    27061 tcggttgatg tccggctgat tgcggcgccc catcgcgatc ttgccgcaga ggtgaatgcc
    27121 gggcgttttc gccaggatct ctactatcgc ctgaatgtgg tggcgattga agtaccatcg
    27181 ctgcggcaac ggcgggaaga tatccctctg ctggctggcc attttctgca gcgctttgcc
    27241 gagcgtaatc gcaaggcggt aaaaggtttt acgccccagc gatggatctg ttgattcatt
    27301 acgactggcc gggaaatatt cgtgagctgg aaaacgcggt ggaacgggca gtggtgctgc
    27361 tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt acgccgatcc
    27421 cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag gtgattctgg
    27481 cggcactgga gaaaacgggc ggcaacaaaa ctgaagccgc ccgtcagtta gggatcacgc
    27541 gtaaaacgtt attggcaaaa ctgtcgcgtt agttctgctc gcgttcgata gcacgccagc
    27601 cgatatcttt ccggcagaag cagtcgtccc agtggatatc ggtcattaag gcataggcgc
    27661 gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg cgcccgccgt
    27721 tggtcactac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact ttgccgcctg
    27781 ccacttcttc cagcggcagg ccgtggatca cgtcaccggt gcggtaatca cccggatatc
    27841 cacccgcagc catcaccacg ccgagagaag cgcgttcatc ccactcggac gttttctcgt
    27901 ccagtttgct ttcacaggcc gccaggcaga gctcaaccag atcggacttc atgcgcagca
    27961 taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc
    28021 cctgtttgtc gatcatcagg cccgcgtaga gaaaaccggt gtaggtgttg ccttccgccg
    28081 ccatgccttt cacggttggc cagataatac gttccatggt gcgctgatga acgtcatcgg
    28141 ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg gtatctttat
    28201 cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg ccgtccacca
    28261 tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg cgatgacccg
    28321 cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct tcttccagcg
    28381 tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg acgattggcg
    28441 cgcctttctc acgcagatac gccagcgcag gttctacctc ggtgaagttc tggtattccg
    28501 ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag ccttccagtt
    28561 gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag gtatcgacca
    28621 cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt tcgttttgtg
    28681 cgaaatccaa cagcgccggg atatcggtca cgccaatagc aacgttttgc agcgcgggtt
    28741 ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc ggcgactggg
    28801 ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat actttcatct
    28861 attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc
    28921 gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt
    28981 cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg cgtcagaagc
    29041 catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg cgattttcgc
    29101 ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt ttttggcata
    29161 gacgatagca ttggatttca cgaacttcgc cactttccag cagaacagcg catcgcgcag
    29221 ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac cgaccatccc
    29281 caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac
    29341 acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt
    29401 cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac gagaaatgat
    29461 ggcctgtgcg gtttccgcat ctagctcgcg gttaaaggca atgatgccgc cgaatgcgga
    29521 ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc cgatagctac
    29581 accgcaaggg ttggcgtgct tcacaatcac acatgctggc tcggcgaact ctttcacgca
    29641 ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac cctgaacctg
    29701 ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg cagcctgctg
    29761 gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg
    29821 tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca tgctgccgaa
    29881 gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc
    29941 gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa taatggcgtc
    30001 gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg cggagcgcac
    30061 catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc agccttcacg
    30121 agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag gctggatctg
    30181 atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac catgtacttt
    30241 cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt aatcggaaac
    30301 ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg tagacagcag
    30361 ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt tgtcagaaac
    30421 actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc tggatttgac
    30481 tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc cctaacgggg
    30541 catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg cgaatttttc
    30601 tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac agaaattaca
    30661 ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat
    30721 gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa ctccctataa
    30781 tgcacctcca tcgacacggc ggatgtgaat cacttcacac aaacagccgg ttcggttgaa
    30841 gagaaaaatc ctgaaattta gggttgactc tgaaagagga aagcgtaata tacgccacac
    30901 tcgcgacagt gcgctaaa
//
LOCUS       DAJTNA010000028        30689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTNA010000028 DAJTNA010000000
VERSION     DAJTNA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30689
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.028"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(148..4608)
                     /gene="mukB"
                     /locus_tag="OO161_001093"
     CDS             complement(148..4608)
                     /gene="mukB"
                     /locus_tag="OO161_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5462243.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFSLTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            complement(4608..5312)
                     /gene="mukE"
                     /locus_tag="OO161_001094"
     CDS             complement(4608..5312)
                     /gene="mukE"
                     /locus_tag="OO161_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5462244.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5293..6615)
                     /gene="mukF"
                     /locus_tag="OO161_001095"
     CDS             complement(5293..6615)
                     /gene="mukF"
                     /locus_tag="OO161_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5462245.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6612..7397)
                     /gene="cmoM"
                     /locus_tag="OO161_001096"
     CDS             complement(6612..7397)
                     /gene="cmoM"
                     /locus_tag="OO161_001096"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5462246.1"
                     /translation="MQDRNFDDIAEKLSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7533..8312
                     /gene="elyC"
                     /locus_tag="OO161_001097"
     CDS             7533..8312
                     /gene="elyC"
                     /locus_tag="OO161_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5462247.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8289..9182)
                     /locus_tag="OO161_001098"
     CDS             complement(8289..9182)
                     /locus_tag="OO161_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5462248.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9336..10082)
                     /gene="kdsB"
                     /locus_tag="OO161_001099"
     CDS             complement(9336..10082)
                     /gene="kdsB"
                     /locus_tag="OO161_001099"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5462249.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10079..10261)
                     /gene="ycaR"
                     /locus_tag="OO161_001100"
     CDS             complement(10079..10261)
                     /gene="ycaR"
                     /locus_tag="OO161_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5462250.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10313..11545)
                     /locus_tag="OO161_001101"
     CDS             complement(10313..11545)
                     /locus_tag="OO161_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5462251.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQVITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11582..12568)
                     /gene="lpxK"
                     /locus_tag="OO161_001102"
     CDS             complement(11582..12568)
                     /gene="lpxK"
                     /locus_tag="OO161_001102"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5462252.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12565..14313)
                     /gene="msbA"
                     /locus_tag="OO161_001103"
     CDS             complement(12565..14313)
                     /gene="msbA"
                     /locus_tag="OO161_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5462253.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14350..16614)
                     /locus_tag="OO161_001104"
     CDS             complement(14350..16614)
                     /locus_tag="OO161_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCR5462254.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRGVHGQLNDGGFDSQRYAIAQHQPL
                     IGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPNEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VYDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16821..17105)
                     /gene="ihfB"
                     /locus_tag="OO161_001105"
     CDS             complement(16821..17105)
                     /gene="ihfB"
                     /locus_tag="OO161_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5462255.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17265..18938)
                     /gene="rpsA"
                     /locus_tag="OO161_001106"
     CDS             complement(17265..18938)
                     /gene="rpsA"
                     /locus_tag="OO161_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5462256.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(19049..19732)
                     /gene="cmk"
                     /locus_tag="OO161_001107"
     CDS             complement(19049..19732)
                     /gene="cmk"
                     /locus_tag="OO161_001107"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5462257.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19905..20669)
                     /gene="ycaL"
                     /locus_tag="OO161_001108"
     CDS             complement(19905..20669)
                     /gene="ycaL"
                     /locus_tag="OO161_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5462258.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMNMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAVNAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(20838..22121)
                     /gene="aroA"
                     /locus_tag="OO161_001109"
     CDS             complement(20838..22121)
                     /gene="aroA"
                     /locus_tag="OO161_001109"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5462259.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(22192..23280)
                     /gene="serC"
                     /locus_tag="OO161_001110"
     CDS             complement(22192..23280)
                     /gene="serC"
                     /locus_tag="OO161_001110"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5462260.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(23479..24171)
                     /locus_tag="OO161_001111"
     CDS             complement(23479..24171)
                     /locus_tag="OO161_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR5462261.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            24301..26061
                     /locus_tag="OO161_001112"
     CDS             24301..26061
                     /locus_tag="OO161_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5462262.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            26467..27324
                     /gene="focA"
                     /locus_tag="OO161_001113"
     CDS             26467..27324
                     /gene="focA"
                     /locus_tag="OO161_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5462263.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            27379..29661
                     /gene="pflB"
                     /locus_tag="OO161_001114"
     CDS             27379..29661
                     /gene="pflB"
                     /locus_tag="OO161_001114"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5462264.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            29853..30593
                     /gene="pflA"
                     /locus_tag="OO161_001115"
     CDS             29853..30593
                     /gene="pflA"
                     /locus_tag="OO161_001115"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5462265.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         7537 a         8238 c         7417 g         7497 t
ORIGIN      
        1 aggagaggcc ctggaaatac cccacagccc cgaaaaagag ccagtcagtt gcttaactga
       61 ctggcattac gccgttatgt gccatttttt tagtgcagaa acggagtttt cggaaaaaga
      121 aaaggcggca ttgctgccgc cttaatttta actcgcctga gaaggcgctt cgtcagttcc
      181 tggaagcgtt tcagggagtt gcggcgcaaa tcctcgcagg ccgacgacat gaacatgttc
      241 ggtattctgg aagactttac gcaccagttt ataggtggtg cctttctccg ggctgatatt
      301 ttccggcgct gcgatgatga gctgcatttg caaacgctca cacaattcaa acagcgtggc
      361 gatagaacga gcatccagtc gcgctgcttc atcgaggaac agcagacggc aaggagagat
      421 atctttaccg cgcaggcggc gagattcatc ttcccagctt tgtaccacca tcaccagaat
      481 cgacataccg gtaccaatcg cctcaccggt cgacaatgca ccagactctg cgcgcaacca
      541 accatcggaa ccacggttaa cctcaacttc catttccaga tagttgcggt aatccagcag
      601 ttcttcgcca atggtctgcg gcgtgcgctg ccccatatca atctgcggat taagacgctg
      661 atacaatttc gccaacgctt ccgagaaggt cagacggttg ctgttaaaca gatcctgatg
      721 ctgctcgtgc tgttcagaga gcacatccag cagcatggcg tgcgtttcac gcacgttcac
      781 gttgaggcgc acgctgttca cctgaccaaa cgatacgttc tgcaacccct ggttgagcat
      841 acggatacgg ttctgctcgc gctgaatggt tttgcgaatg atgttcgcca cgctgcggga
      901 actgatcacc agtttctgtt cacgggaggt taattcttcg gtcagacggc taagttcgat
      961 ctccatctgt tcgatagctt ccaccggatc atcggtacga ataatatcct gacgaatacg
     1021 ttcacgcaga tgctgataag ccgccacgaa gaactgaatt ttacgctccg gacgtttcgg
     1081 atcttccgac atgcgcagca cgtcgcgcag atgttcgtta tccgccaccg ccagacgcag
     1141 cgcacccaat gccttatccg acatagagcg caaatcatca gcggagagat aagccagctc
     1201 acgacggtgt aagcggcgct caacgccgtt atctttcacc atgcgcatca ccgcacacca
     1261 gcccgctttg gcggtcacta cctgctcgcg catctcaaag taatcccgct ccagcttgcg
     1321 cagtttgcgg gtcaggttgt ccatctccgc ttcgcagaag gtaagcgctt tttccagttg
     1381 attgcggcgt gaacggttat tgctcagttg cgcatgcagc tcgtcacggc gaatacgcgc
     1441 ccgctcttct gccccgctat cagcacgcac gccgatatcc tgcaattcac gttgcagatc
     1501 gttgagtagc tcttttttgg tgtcgtaaga acttttcagc gaagccagca cctggttgta
     1561 ctgactcaac tgcgctgcgt gaccgcgcaa cgcttcgcga gcgcgggtac gctccgcttc
     1621 cgcctgttcc agacgttcac gcagtttttc gttgagatcg ctgttaccgc taagcatttc
     1681 tgccgagtca gagtagctaa agtgcgcacg acgctgcacc acttccgtca gggaaaacgc
     1741 ctgctgacgg gcatcgcgct gcatctgttg agagtacgcg taatcttctt ttaactgttc
     1801 gaactgttcc gggtcgctct gcaataccga aacgatcggt tccagtttcg ccagttgatt
     1861 gccaaactgc tgaacaaaac gcgcggcttc ctgggcttca tccagacgtt cgcggatttc
     1921 atcgacgcga tccgccaggc tatcatcagc caacaggttg agacgcggca gaatgcggtt
     1981 cagcgccgta acgccctctt tcgcctgctc aaactgaata cgctgctgct ggttatcatt
     2041 ttcatgatta cttaacgccc gctccagttc gacgcgacgg ctgttcagtt gacggatttc
     2101 tgcttccggg tcagactcaa acacaaccgc cagatgactg ccgataaagc ggctgaacgc
     2161 ctgatgcaga cgctgagttt tctgtacatc aaaggagagc gtggcgaagc gttcggaaag
     2221 cacttcacgc tcggcatgga ggctttcaat acggctttca cgcgcagcac gaccaaacag
     2281 cggcacttcc gggaaacgtg aataacgcca ctgacgatcg gcgattttca ccactaccgc
     2341 tttttccagc tcatcaacgc tgaacacgct gtcatcgaat gactgcggat ctccttcgat
     2401 cagatagaga tcttccgggc aatcggtcaa gccatccagg tgttcagtta cctgtgacag
     2461 atctggcacc acgatggcgt ggcgtgacgg gccatacagc gctgagaagt acggcgcatc
     2521 ttccaggcta acgtcgtcat aaatttctga cagcagcaca ccaccaaaac gctccgccag
     2581 cgcgttcaga cgctgatctt cagagccgcc aggctggctt aaacgttcga tctcttcatc
     2641 gacggcgttt ttgcgcgcgc ccacttcatc gcgttcaaca atcgcctctc gctcacgctc
     2701 cagcaactgt tgcagatatt cggtgacgtc ctggctggag gtaaactctt cgccgcactg
     2761 ttcgctcaac tggttgagac tgttttgcgc tgccagccaa accggcgcac gctgcatcaa
     2821 actctgaatg cgagactgca gctgttcctg ctcctggcgc agtgccatgc gctcttcacg
     2881 ggcgttagac acgctatcgg aaagagaggc aatgcgtgct tccagttcct gatgcagggc
     2941 ttccagttcg tcgatatcaa aattcttgcc ctgacgtttg cagaaatctg ccagcagacg
     3001 ctcagcttct tgctgctcgc gtagacgctg ttccagttcg cttaatcgca tccgcagcgg
     3061 ctgaacctgc tctgccaggt gacgctgatc gaccccttcg cgcaatagtt cgcgagcgac
     3121 atcccacgcc tcgttacgcg ccagtgggcc gttgattgcc accaccagct gataagcctg
     3181 ctcaaactgg ctgtgcgcgg tttgcgccat gctcattttc tgctcaagag agagcatttt
     3241 ttcagtcgct tccagctctt tcgcctggaa ggtttccagc cattcggcgg cgctgtcggc
     3301 ggttaagtcc ggcagatggc acagttcttt ggcacgatta agcgcagcaa tcgcctggtt
     3361 atactggatc gcgcgcgttt gctggacgtc cagcgcctgc tggtagtcag caagctggct
     3421 tttcagctcg tccacttcca gttcggcagc ttccgcgcga gcctcgttct cttcctggcg
     3481 ttcgatggct tctgccacca cttcgttttg ctcttccaga cggatctgca gctcatcgag
     3541 atccgcttcg tagcgttcga tcttctcctg ctgacgcagt gcggtttgca ccaggttcag
     3601 gtgatcgctg gccgcctgat aatccgcttc cagatcgcct tcggcaccgt tgtgctctgc
     3661 cagctcacgc gccatatcga cgtgtttgta ctgctcagcc gccagttgct gacgcgaagt
     3721 atgtaactcg cgacgaaact ccagggcttt gtcgagatgg acacggcgct cgttggcgtg
     3781 acgcatgtag tccgccgcca cgtagttggt agcttcgctg atcagatgct taaacaggtc
     3841 acggtccgac tgggtgacac gaatcgcttc cagcgtcatg cggttttcac gcagcgccgc
     3901 ttccatgtcc tggaacgctt tacgcacgcc gctgttttcc ggcaacaggt agtcgcgcag
     3961 agaacgggta atggcactgg agatcccgcc atacagcgaa gcttcgatca gacgatagaa
     4021 tttgctacgg tcagatgcgg agcgcagacg acgcgcgatg atgcccagat cgaacatcag
     4081 cgagtggtaa tcggtaatgg agttgaactg tttaaactgc acgccttcca tcgcctcgag
     4141 cttgtctttc agctcgttaa gcggcagcac gcgcgcctgg cgttcgttca gggtttcggt
     4201 caccagctgt gtcggctgca ccgacatcgg cagtccctga atggcaaatg gtttgatatc
     4261 cactttacga tcgcgtccgg caacctgttg cagacgcaca ccgaccacca cgcgctggtg
     4321 gcgcgagtta atggtgtcga gcatcgaata acagacaccc gctttcagct taccgtgcag
     4381 acctttatcg cgcgaaccgc tggtggcccc ggcttccgta gtgttacgga aatgcagcag
     4441 ggtcaggtcg gggatcagcg ccgtaacgaa cgccgccatg gtggtggatt tacccgcccc
     4501 gttaccgcca gaaagcgtcg tgaccagctc gtcaaggtca aaagttcggg caaaaaagcc
     4561 gttccagtta atcagcgtca gtgagcgaaa tttaccgcgt tcaatcatta ttcttcctct
     4621 ccgctatctg gctgattctc ttcggtttca tcgttgagtt gcagatgatt ttcaatcggc
     4681 attgcttcgc catcacgaat caggcgacgc tgtgcttcac ggggatcgtc gccagcacgc
     4741 acatcggctc cgaagcggaa caccgattcg gtaatgcgaa acttgctgct gtcgtggccc
     4801 ataaaccaca ccatgcctaa acgacgcaga cggttgagcg aagaacgtac tttctcctgc
     4861 aacttctgac ggtcaacgtc tgaaccggtt gaacggttgt tcaccagttt cagcagtttt
     4921 gcttcatcgg ccagggtgag cagttcgtcg tacagttcct gctgggtgaa aatcccctca
     4981 ttcgccagcc gttccgggct gagatagaga taacagagga ttttcccgac catcatatcc
     5041 agttccgaca agacggaacg cgggataaga gtggtggaac gtgggcgtaa atagaagaac
     5101 ccttccggtg cgcgaataag ctcaacgtta taacgcgcgt aaaactcttc cagatattcc
     5161 tgaaaatcca tcaggaatgc atgattatcc agttcgtcga ggccaatatg gcggcctgaa
     5221 cgtaaggcgc tgtccagcgc cggaaataac ggattcgcca gcgcctgcgc cagcttaacc
     5281 ggcatcactt gttcaatatt tgtcgatgac atgcgcctgt accttggctc cgtaatcatt
     5341 aatcggctgc catttcgctg gcagtccggt gaaatctgct tgcgctacgc caagacgtac
     5401 cgcctgatca ataacaatac gcgcaacgtc aaagtgacgt gcacgcggat actgtgacag
     5461 atattcgcgt accaccagac caagatccag cggcacttgt ctggttttgt acacggcaag
     5521 ttgttcttcg atgatcgccg ccagctgttc gcggatctcg ttaaactctt cgtattccag
     5581 atcctcagga agttccccag tcacttcttc atcgcgcagt gccatctctt cgtcacgcat
     5641 atccagcaga cgatcggcat tggcataagt tagcgcccac ggctcatcaa aataggtttg
     5701 taccgactga cgtaaccgct gagcaaagac gcggttttta tccatatcga tcgcggtacg
     5761 aataaatttg tgtacgtggc ggtcgtagcc aatccacaag tcgatggatt gctggcccca
     5821 actgataata cgatcgagtt tgctctgcag atcgaacacc agacgatcga cgaaatgcag
     5881 atcgtcatgg gtcatcgtcg catcctgaat gcgcaacaga ttagcctgca atttgtcgcc
     5941 tgccgcttcc agcgtatcct gcaattcacg cagcgttccg gaagtttccg aaagcaacaa
     6001 ttcacagctg gaaattgccg cccgccagtc tttgttcagc aactgggcga tatcgtcctt
     6061 cacctgctgc tgctgttcgt ccatcagacg ttgcgtcagg tcgatactgt cgaaaatttc
     6121 tgctaccgag tatttcagtg gcgcatagac attacggtgc cagtgaaatt catcaccgcc
     6181 ctcttcggcg gcatctgctg cgcgtttgag ctcacccgcc acaatcgaca actgcataga
     6241 aagacgcagc gtagaaaact cgcgctgacg gatgtagtag tcagtaatgc cgatgccgag
     6301 cggcgtcaga cggtaaattg cgttcccttc cgcctgctcg ctggtaaagc ggttcagcag
     6361 acgttgacgc accatgtcgt tgatcgcgtt attggcgcgc acgccgatgg tttcgctggt
     6421 ttgctcaaac gcatcactca catggcggaa tgcatccacc agctcgcctt cactcatctc
     6481 accatccaga cgctcgccgt tcagcgtggc aaccgccagc agaaaagaga gtcggtctac
     6541 cggcagcgag atggagaagt cattttttct ggcccaggca accagttcgg ggactgtctg
     6601 ggaaaattca ctcatacttt atccttgctc tgcggtttgc gcgcggtgac atgaatataa
     6661 cgccccaggg taatatacgg ttcctgacgg caataacgcg tttctaattc aagtaatgct
     6721 tcatagcagt cgcgctgctg gtgcttctcg cgcagataat catgaaacac gcgaacgcct
     6781 gtcttaccca taatttgcca accagcttct tccagccaca gataaacctg cgccgggtcg
     6841 cgtggataat ctggcgaaag cgtccgtttt ttctttttcg gcattcccgc ctgcacgtaa
     6901 tcaaaattcc cggcgaccat gttatgcatc aacaaaccat gcgcattgta gaacattaac
     6961 gacaacacgc cgcctggacg caacactgac cacagggtct gcaatacact gcggggatcg
     7021 gccacccact cgagcaccgc atggaacaat atcagatcaa cgggcgtttc caaatgcgaa
     7081 gcaacatcct gagcggcgca atgtataaat tgcatgttgt cgctcacacc ttttgcttct
     7141 gccgcctgtt ttgcgcggtc gatcatctgc gcagaaagat cgcataaaat gacctgatgc
     7201 ccacgctcgg ccattttgat tgcggtctgc ccttctccac cgccagcatc cagcacacgc
     7261 agtttttgcg ggcccatttc cgccagcacg cgatcgagat cctgccacag aatagcctgt
     7321 cgaagctgcc ctttggtggt gccgtaaatg ttacgggaca acttttccgc aatatcatca
     7381 aaattgcgat cctgcattgc tttctccacc ggttggctaa gacgttttct gataaagacg
     7441 ctattttgtc acagccacgg cgagaatgaa cccgtctggt gtaatatcgc tggcgatccc
     7501 ttgctatatg gttaaaaaag gaaccagaaa ggatgctttt tacactgaaa aaagtgattg
     7561 gcaatatgtt gctgccactt ccgctgatgt tgttaattat tggcgctggc ctggcgctgc
     7621 tgtggtttag ccgttttcaa aaaaccgcga agatttttat tagcattggg tggctggcac
     7681 tcttgttatt gagtttgcaa ccggtagccg accgcctgct gcgtcccatc gaaagcacct
     7741 atccgacatg gaataattcg caaaaagtgg actatatcgt ggtgctcgga ggtggttata
     7801 cctggaaccc gcagtgggca ccgagctcta atttaatcaa taacagtctg ccacgcctga
     7861 atgaaggtat tcgcttatgg cgcgaaaatc cgggatcaaa actgatcttc acgggaggcg
     7921 tagcaaaaac caatacggtg agtacagcgg aagtaggtgc cagagttgcg caatcgctgg
     7981 gcgtgccgcg cgagcaaatt atcaccctgg atttgccaaa agataccgaa gaagaagctg
     8041 cggcagtgaa acaggcgatt ggtgacgccc ctttcctgct ggtgacctcc gcatcacatc
     8101 tgccgcgggc gatgatcttt tttcagcagg aagggttaaa tccgctacca gccccggcga
     8161 atcagttggc catcgactcg ccgcttaatc cgtgggaacg ggcaatccct tctccggtct
     8221 ggctgatgca tagcgatcgt gttggctatg aaacgcttgg ccgcatctgg cagtggctga
     8281 aaggtccgtc aggcgagcca cggcaagagt gattttgatg ccagatcgaa gttgcgccga
     8341 ctaaatcgtc cggtattaac cagttgcgca acttcatccc ataacacata caaccaacgc
     8401 cgccagatga acgactccgc cactggcgcg cgttgcaggt aactccagag caaatcttcc
     8461 gccagagaat tatccattag tcggaacagt tcgtattctc gcggtgccca aagcattagt
     8521 cccggcccga ccatcgccag taactgatcg ctgcgcgaat ctttcagcat gctgcgtaaa
     8581 cagaagttac cgtgaatcaa cacacaattg tcgttaaagc cttcaaataa tgccgggaga
     8641 cattcgcgag tgcgaaacag gatccgctta tcctgcatcg tcagaccggt gttattgaac
     8701 tgattgagcg tggtccatag cacttcaaca tgttgccggt accatgaggg ccagaaattt
     8761 tcctgagtat tgtcgaccgc accgacgcaa ccgcgactgt cctgacggtg ccaggccagt
     8821 aaggcttcaa cgatttggtc tttgagctgt tcccagcgtt ctggtgttcg ggctggtgcc
     8881 tccaccgaaa caccacgcat ccgctccagt agcaggacat cagggccggg gtgttcttca
     8941 tgcgtcatca caccataaat agtcggcata cggacagtcc cacttcttgc cagcatggtg
     9001 gttttccatg ccagttgtcg ggcttttccg ggcgtactaa agcttcttgc cattaacggc
     9061 attgggtttc cctggctgtc atacaaagcc cacaacgccg tatccgcttt ttcattgacg
     9121 cactcaatac gactgagttt ttcgcccagt aaatggctta attcggcacg cagctgttcc
     9181 attcagatta ccctcataaa agctactgct ttcataatga gcgccaatat aaaagatgtc
     9241 acctgaatga gatcaattgt tgaaaagaaa aattgaggaa aatcgtaacg gtacgacact
     9301 cctcccaaaa ttggctgaag tgtcgtgaag tgaaattagc gcatttcagc gcgaacgcgc
     9361 tcaagatctt caggggtatc cacacctgtg ccaggaactt cctgagcaac agcaacatgg
     9421 attttttcgc cgtaccacag aacacgaagc tgctctaaca tttcgatgtg ttctaacgga
     9481 cttgcctgcc agttgacgta acgacggata aagcctgcac ggtagccata aatgccaaga
     9541 tgacgcagga agttatcgcc aacggtttca aggccttctg caaaacgatc acgatcccaa
     9601 ggaatggtgg cgcgtgagaa gtacagtgca tacccttcag cgtcgagaac cactttcacc
     9661 gcattcgggt taaacgcttc ttccgcattg tggattggca ccgccagagt cgccataccc
     9721 acctgacgct gagcgaggtt atcagcaacc tgacgaatga ttgtcgcagg gatcatcggt
     9781 tcatcaccct gcacattaac gatcaccgtg tcgtcgctga atgcgcattt ttcgacaact
     9841 tccgccagac gttctgttcc tgactgatga tcggcgcgcg tcatacatac ttcaccgcca
     9901 gcggcttcaa cggcgcgggc aacatcctca tgatcggttg ccacgatgat gcgctcggca
     9961 cctgattcac gcgcgcgttc aagaacatga acaatcatgg gtttgccgtt aatatcaacc
    10021 aatggtttac cgggcagacg cgtggacgcg tagcgcgcgg gaataatgac cacaaaactc
    10081 atgatttact ctcatcagca gtcagcacgc gggcttccgt ttccagcaac actggaatgc
    10141 catcgcggag cgggaaagcc aggttatcca gtttgcaaat aagctcttgt ttttcctggt
    10201 tataccaaag ttttccgttg caaaccgggc aggcaatgat ttcaagcaaa cgatgatcca
    10261 tagttcctcc agatggatcg ggttatgaat gcataaatct tatcataatc atttatgcga
    10321 cggggtctat ttcccagcca gtgcggcaat cagtaaagag accttgcggg cagcgtccta
    10381 atgtcacccg cgttgcctgc tgccagctcg cgaaatcagt aatcacctga cgtaaccctt
    10441 tttgcagcat tgtcgttggt ttaatgcctt cctgtaacca cagagagata acttcaagga
    10501 tgcctgtctg gcgatgcatt ttggcatcca ttcgcccaac taattgccca cgatgtaata
    10561 atggcagaac aaaatagcca tactggcgtt tcggcgctgg ggtatagcac tccagccggt
    10621 agctaaaatc aaaaagctgc tctgcgcgtt tgcgatccca gacaacagga tcaaaaggcg
    10681 aaagtaccgc gctgtgcgtt gcagtgagct ttccggccag cgcacgctcg agtagcggca
    10741 gcaaatcagc atgcagccaa agattgccca atttttcaac atgcacagca atgatttgct
    10801 gctgttcagc cctcgcttcg cgccacgccg ccagtgccgg acgtttcagc cgatagtaat
    10861 ctgccagcca ctgttcgcgg aatattccca ggctacgcgc actgttatcc agcatgatga
    10921 tttctgcttc tgtttgcgaa acgagatcgc gctcatcatc ccagtcaggc atgacacggt
    10981 gggttaaatc ataaacgcgc tggaagttgc gccgttcaat caccatcacc tttccggcag
    11041 taaataaacc ttccagatgc cgtttatgcg gcttccattc ccaccagccg cttgcacctt
    11101 tacgaggatg ctcaaaatcg gctgaacgta ccggcccctt atcatgaata tgctgaatta
    11161 actgtgcaat ttccgcctca tgttcctgca tccaggcgtc tttgtatttc cagcccattt
    11221 tttcaggtgc cagcatgcgg tggcgaataa gacgaaagtc gctacgcggc ataaagcagg
    11281 cttcatgcgc ccagtattcc attaattcgc cacgcgccag agactcatcc agccactggg
    11341 caggataatt tcccagacga ctgaaaagca ccagatatgg actacgggca acaatattga
    11401 tggtatcgat ttgcagcaag gacatgcggg agatcgttgc cggaatatcc tccaacgacg
    11461 ctcgacggcg gggtttgttt aacaggcctt gtgcggcaag gtgaagatta cgcgcatcag
    11521 caagggagag gtgcggcagc gacattcatg actccatcaa tcgaacgctg ccgcggcgta
    11581 actagttgcc agaagccagc gaggttagtt gcgtaagcag tttcgctggt tcatcacctg
    11641 aaagctgtgc gtctacaggc aaataccacc aattttcttc tgcaaaggcc cggcatttca
    11701 ccgcatcttt ttcagtcatt accagcgttt gcccggcgct taccaacgca ctgacatccg
    11761 catggttcaa agactgatga tcggccagcg gtacacattt ttccggttgt acgccacaca
    11821 tcttcagcgt ggcaaaaaag cgcggcggat gcccaatccc cgccatcgcc actacatgtt
    11881 caagctgagc aacgtcacaa cgcgtaccgg tacgtaaatt caccgcctga cccggcagca
    11941 gatgcatggg gatttcaccg ctgcgaggga caccgccgtt gacgattacc gcatcaaccg
    12001 actttaagcg ccccgctcgc tcacgcattg gccccgccgg caaccaccag ccattgccaa
    12061 agcgacgcac accatcaata acgacaattt ccacatcacg cgccagacgg taatgctgta
    12121 aaccgtcgtc ggttacgatg atctgcacat cagggtgttg cgccagaatg gcttttaccg
    12181 catcagaacg aacgggagaa accgcaacag gcgcatcagt gcgttgataa atcaacacag
    12241 gttcatcacc cgcctgtgct gttgtggtat ctgccgacaa taacagcgga taagattcag
    12301 ccttactacc atatccccgc gatacgaccc ccacgcgaat accgcgctgt tgcaactgtt
    12361 ccaccagcca gacaacgacc ggggtttttc cgttgccgcc tgcggtgaga ttaccaacca
    12421 cgacaaccgg tacgggggca cgccaggcgc gcttcagttt tagtttatag caaagacgga
    12481 tcgcgccact caccaggcca tacaaccagg agagtggcag caatagccgc cacaaagggg
    12541 attcaccaga ccagattttt tcgatcattg gccaaactgc attttgtgaa gttgcgcgta
    12601 aacgccgcgg tgctcaagca aatcgttatg cgtaccgcgt tccacaatga caccatcctc
    12661 gacgaccacg atttcgtctg ccttttcaat ggtagacaag cggtgggcaa tcaccagaga
    12721 ggtacggttt ttctgcaact catccagtgc cgcctgaatc gcacgttcgg attcggtatc
    12781 cagagccgag gtagcttcat ccagaatcag aatcgggcta tcacgcagca aggctcgagc
    12841 gatagcaata cgctgacgct gaccgccaga gagcagcacg ccgttttcac caatcactgt
    12901 atcgagaccg ttatccatct tattgatgaa gtccatggcg taggccatac gcgccgcttc
    12961 ttcaatttgc tcacggctgt actgttcagt ccgtgcgtaa gcaatgttgt tagcaaccgt
    13021 atcgttaaac agatggacat tctgcgacac cagagcaacc tggttacgta acgacgccag
    13081 ggtatactcg cgcagatcgt gaccatccat caggatttcg ccttcatcaa tatcgtaaaa
    13141 acgagtgatc aggctggcga tggttgattt acctgaacca gagcgtccaa ccagagcaac
    13201 cgtcttccct gccggaattt tcaggttgat gttacgcaat gcaggtacgt cacgtcccgg
    13261 ataagtaaag gtgacattgc ggaattccac gtcgccagtc gcacgctcga tcacgcgctt
    13321 accttcgtct ttctcctgct cactgtccag aatggtaaac agcgtctgac aagccgccat
    13381 accgcgctgg aactgggcgt taacgttggt cagtgatttc agcggacgca tcagtgcaat
    13441 cattgaagag aaaacaacgg taatcgtacc ggcagtcagg ctatccatga cacttgggaa
    13501 gctcgccgca tacagaacaa acgccagcgc caaagaggcg atcagctgaa tgatcggatc
    13561 agagatggaa gaggctgaaa ccattttcat cccctgaaga cgcattcggt tgctgacttt
    13621 atcaaagcgt ttcgtttcca cttcctgacc accgaaaatc aatacttctt tgtggccctt
    13681 cagcatttgt tcggcgctgg tggtcacctg ccccatggtg ttctgcatgt ttttactgat
    13741 gttgcgaaaa cgcttcgata ctacgcgaat cgcaatcgaa acaatcggtg ccagcacaat
    13801 caaaatgatc gacagttgcc aactgtaata gaacatcatg atgaacaggc cgatgattga
    13861 cgcaccttca cgcacaaccg ttatcagtgc gccggaagaa gaagaagcaa cctgttcgga
    13921 atcgtaggtg atacgtgaca acagcgtccc cgttgactgt ttgtcaaaaa atgaaaccgg
    13981 cattcccatc atgtgaccaa acaggcggcg acgcatggtc attaccacct ttcctgatac
    14041 ccaggagata cagtagctgg agacatagct ggtgatacca cgtaaaatca tcagcccgat
    14101 caccaccagc ggcatccaca ccagcacgga gcgatctgtt ttaccaaagc catcatcaag
    14161 aagtggctta aggagcgata acatgaaggt atcgctggct gcgttgagga ttaacgctac
    14221 gcccgccacg atcagacccg ctttaaaagg cgcaatggtt ggccacagtc ggcggaacgt
    14281 ctgccacgta gagagatctt tgtcgttatg cattcaaaaa accagcattt gttgaaatag
    14341 ccgcatattc tacccgttat ccactggcac gccaaaccac tgatgatacc aacgaggtaa
    14401 aatttgctct ctgaggctgc taatccgcca gccttgcgct gaaaaattga ccgtcacttg
    14461 accctgatgt ggagtatcaa gccattgata tccttgctgt tgatagcgat gcttaacttt
    14521 attagagggc aatcgccatg cgttatagcg cgatgccgat gcgagtgcca cttttccatt
    14581 cactcgctga attaatggca acgatgatga ggtattactg ccatggtgag gtacctgaag
    14641 caatgttgtc tgcacttgct gccagtaacg gcttagcatc ttttgttcag cggggacttc
    14701 aatatcaccg gttagaagaa tgctattcgt cccgtcataa accttaacca cacaggaatg
    14761 gttatttcct ttatcgttgc tagcttgtaa aggccagtgc acgctgaaac gcaatccttg
    14821 ccattgccac gcttcgccac gcacacaggg ctgatgatgt tcccagttta acggacttct
    14881 gatccataac atcggccatg tgtgcaatat tgagtccagc cctccccggt gatccagatg
    14941 ttcatgactc agaataacgc cttccggttc aagattatgc cagtggagcc aggggataat
    15001 cagttgttgc ccactatccc cttcgggcca ggccagtcct gtgtcataga gaatcgcttt
    15061 gccgtttctg gctatcacca ttgccagccc ttgcccgaca tcaagcatgt acacctgcca
    15121 ctcgttaggt cgaggttttt gccacagcgg ccagcacatc agcaagccta cagccacaca
    15181 cattgctggc aacgttcgcc aggcgtttaa tcgccatacc accagtaaga accatgggga
    15241 aaatgatagc cattgccaac gttcagcaat gttgatccac ccttctggca aactctttaa
    15301 cccccagaaa agtaaagcca aagaccggtc ggcaagaaac cataaccctt gctccaggat
    15361 taacggcccg cttaaatgca caaccatcgc ggcgaggatc aacggaaccg tgataaatgt
    15421 caccaaggga attgctaata gatttgcaat aaacgaggtc agactaatgc catgaaatat
    15481 aacgatttgc acaggcataa gcaggagtgt gattcccagt tgcaaatgaa tgagggaaac
    15541 aactgcacgc aataccggcg gcagttgcca ctcaggacag ggaaaccact ggtaccaaaa
    15601 aatcagtgcc gcgaccgcag cggcagagag ccataaactt tgcgagagaa tggcaacagg
    15661 atccatcagc aaaattgccg ccagacaaca tatccataca tcccagccac tccactgtcg
    15721 cccacttaac ttaagcattc cccacgtagc aagcgccacc acggtacgca atgcaggagg
    15781 ttgcattccc gtcaaccagg cataaaaagc agcacagcag attccaccaa ttaatggcat
    15841 ttgccagtgg atccagcgcc caggcagaaa aacttgccca ccgcgaatga gtccggcagc
    15901 cagcaacgcc gcaaaagcga tgtgcaatcc cgatatcgcc attaaatgcg ccgttccagt
    15961 atcacgcatg atatttttga tttctttggg gacggataac cgttccccca tacctaaacc
    16021 aagaataacc gcattccaca tatagggttg cagcgttgtt tgtagtgacg ccagatactg
    16081 tgcacgcagg ctacaattcg gttcaatgac acttgcctgc agaaagcggc cgatgagcgg
    16141 ctgatgctgg gcaatggcat aacgctggct atcaaagccg ccatcattaa gttggccatg
    16201 aactccacga actttgagtt tcattgacca ttgttgtcca gcacaaaccg cttgcggaag
    16261 atattcgcca tacagcacaa ggcctggcgc agggaatata cgtcgacctt gtagatgagt
    16321 aatttgaccg taatgagtgg tcatgccgtc agttgcagtg atctcaacaa ttgcatcctg
    16381 cgtcgcgcca gttaaggttt ctcctgccca caaaatttgc tttgctgata atatgcccca
    16441 aacgaaaaac agcaacgtca gcgcgatata acggacggtt ttaacaggga taaatgccag
    16501 cacgcaggcg aagagagtca gaaacgcaag ggttaatgtc ccaggcaatt ggggcaaaat
    16561 cagcaacgga aaaattccgc aaattatgca tacgccgact gtcgttattt tcatgcaccc
    16621 ctccctgttt atggaaagtg tgcaactttg ttgagcggtt gtctggtggg aattttgcgt
    16681 tccccgaggc acattcagaa gaatttaacg aaagggtgaa aactgctcaa aactctgcga
    16741 ggcagcttcc agttcctgat tagagaaatg aaaaaagcac ccgataggtg cttttctcgc
    16801 gttcaagttt gagtaaaaac ttaaccgtaa atattggcgc gatcgcgcag ttctttacca
    16861 ggtttaaagt gaggaacgta ttttccttcc agttctactt tatcgccagt cttcggatta
    16921 cgtccggtac gtggtgcgcg gtagtgcaaa gagaaactgc cgaaaccgcg gatttcaata
    16981 cgctcgccct gcgcaagagt cgaggccata tgctccagca tctcttttac tgcatcttca
    17041 accgtcttgg cgggaatgtg cgattgctgg gtggcaagtc tttctatcaa ttctgacttg
    17101 gtcatgattc ctccggttcc ttaaaggcaa attaagcggc tgctgttgct gcattgatta
    17161 agggcggccg tagccgccct tagtgcttga ttacaggacg aaacctgcaa tctgtcaagt
    17221 aaactcaaca aacttcggaa taaaaatccc gaagagtcag agaattactc gcctttagct
    17281 gctttgaaag cttcagccat tgcgttgttg gagaagtttg catcttcctg tttgttaaca
    17341 gttgcgattg catctttctc gtcagcttcg tctttcgcac gaacagacag gctgattgcg
    17401 cggtttttac gatcaacgcc ggtgaattta gcttcaactt cgtcgccaac gctcagaacc
    17461 agggtagcgt cttcaacgcg gtcacgggat gcttcagaag cacgcaggta accttcgacg
    17521 ccgtcagcca gttctacggt tgcgccttta gcgtcaactg cagttacttt accggttacg
    17581 atagcgcctt tcttgttcag agcaacccag ttgttgaacg gatcttctgc gagctgttta
    17641 acgcccaggg agatacgttc acgttctgcg tcaacctgca gaacaactgc agcgatttcg
    17701 tcgccttttt tgtattcacg aactgcttct tcgcctgcaa cgttccagga gatatcagac
    17761 aggtgaacca ggccgtcgat gccgccgtcc aggccgatga agataccgaa gtcagtgata
    17821 gacttgattt taccttcaac acggtcgccc ttgttgtggg tttccgcgaa ctgctgccac
    17881 gggttagctt tgcactgttt cagacccagg gagatacgac gacgttcttc gtcgatatcc
    17941 agaaccataa cttccactac atcgccaacg ttaacaactt tggacgggtg gatgtttttg
    18001 ttggtccaat ccatttcgga aacgtgtacc aggccttcaa cgccttcttc gatttcaacg
    18061 aagcagccgt agtcggtcag gttggtcacg cgaccagtca gtttggtacc ttccggataa
    18121 cgtttagcga tagctaccca cggatcttcg cccaactgtt tcaggcccag ggatacacgg
    18181 gtacgttcgc ggtcgaactt cagcacttta acagtgattt cgtcgcccac gttgacgatt
    18241 tcgctcggat gcttaacgcg tttccaggcc atgtcagtga tgtgcagcag gccgtcaacg
    18301 ccgcccagat caacgaatgc accgtagtca gtgaggttct taacgatacc tttaacttcc
    18361 atgccttcct gcaggttttc cagcagctga tcgcgctctg cgctgttttc ggattcgata
    18421 acggcacgac gagaaacaac aacgttgttg cgcttctgat ccagcttgat tactttaaat
    18481 tcaagctctt tgccttccag gtgcagagtg tcacgcaccg gacgaacgtc taccagagaa
    18541 cctggcagga acgcacgaat accgttcagc tcaacagtga agccgccctt aactttgccg
    18601 ttgataacac cggtaacagt ttcagcatct tcgtaagctt tttccagcgt gatccaggct
    18661 tcgtgacgtt tagctttctc acgggacagc agagtttcac cgaagccgtc ttctactgcg
    18721 tccagagcaa cgtcaacttc gtcacctacc tggatttcca gctcgccctg ggcgtttttg
    18781 aactgctcag ccgggatggc ggactcagat ttcagaccag cgtcaaccag tactacgtct
    18841 ttgtcgatag caacaacaac gccacgaacg atagaacccg ggcgggtttc gatttctttt
    18901 aaggactctt caaagagttg agcaaaagat tcagtcatgt ttaatcttca ggtttatatt
    18961 taacgtccac ctggctccat gccggatggg gttgtttaac atacccgctg tcattccatt
    19021 gcaacggggg tactgcaaat tcggtcgctt atgcgagagc caatttctgg cgcgcgtatt
    19081 gtagcgcttt ttcaatcact tgctcaatgc ttaaggtggt ggaatccaac actaaagcat
    19141 cggctgccgg aaccagtggc gctaccgctc ggttacgatc gcggtcgtcg cgttctttga
    19201 tctcggccaa aaggcgctca aagttaacac taaagccctt ctcctgcaac tgtagcatgc
    19261 ggcgatgcgc acgttcttcc gaggaggcgt caaggaaaat tttcactggt gcatcaggga
    19321 ataccaccgt tcccatgtcg cggccatcgg caatcagacc tggtaattcg cgaaacgcgc
    19381 gttggcgacg caataatgct tcacgaacgc gtgggaatgc cgcgacttgt gaagccgcat
    19441 tcgccacttc ctgagtacga atttcgccgc tgacatcttc cccttcgagg atcacttcca
    19501 gattgccatt ggttgacaca aaacgtacat ccagatggga tgccagcggt accagcgcat
    19561 cttctgacgc aacatcaaca tggtgatgta atgccgccaa tgccagtacg cgataaattg
    19621 cacccgaatc cagcagatgc cattgcaatg cttccgccat agccttacac aaggtgcctt
    19681 tccctgcacc gcttgggcca tcaatggtaa taaccggggc aattgccgtc atctttatct
    19741 ccttaaaaca ggcgtaccgt taacataacc gcgcgcatta tacgcgccga cgccacaaat
    19801 cgttaacgtt gtgtgcaaaa accagttttg tctgtattca gatggggatt tattgcgcaa
    19861 ttatagcggg ctggcagaag accagcccga aagatgacaa tgatttactt accagaggcg
    19921 atacgatcac ggatgtgttg cgcacgctct gttgatggtg ggtgagagtc aaacatggac
    19981 tgggtgcgac cgccatccag actagccagt ttttcaaagc tgccaaccag cccctgggtg
    20041 ctgatgccac gtttcttcaa cagatcaaag gagaaatcat ctgcttcgga ctctttatca
    20101 cgggagtatt tagcattaac agcgccttct gcgatatcac ccaattgtga gcgggaaagc
    20161 tgggaagcca caccgctggt agctgaaatg gcatcgcgtg cggcaacgat cgcataagaa
    20221 gctttcattt cagccagcga gtgacccaac gcgacgtggc ccagttcatg gcccagaaca
    20281 ccttcaattt cgttgtcatt catcatgttc atcaggccac tgtagacacg gacacagccg
    20341 ttcgccatcg cccatgcgtt gacgtcgctg gtcatataaa ccttatagtt gaccggcgtg
    20401 ccattaatat tgttacccaa tgctttggcg atttttgcca gacgtttacc gtacttgctg
    20461 gatttacttg cgacttgatt gccgctgtcc atttcggcac agccctgatt ggcaatcgct
    20521 tttgcatcgg catcgcttaa tgttgctgct ttataggcat ttaaaccgga gctgatagcc
    20581 atattggtat caataccgtg ggtattttga caccctgtca gtaatgctgc agaggtcgca
    20641 atcgccagca gtaatttagt attcttcatg tgaatcatcc gttgattaat catacataaa
    20701 cattatatat caacaactta cattaacaag cgacgctacc attaatcaaa atgactcaca
    20761 aggtccgaaa aaaaacgccg acaatgtgcc gacgttttga taaagaaaga tttggctatt
    20821 tattgcccgt tgttcattca ggctgcctgg ctaatccgcg ccaactgctc gaaataatcc
    20881 ggaaatgttt tggccgtaca tttgggatca agaatcgtca ctggtgtatc tgacaacgcc
    20941 accagcgaga aacacatcgc catccggtga tcattgtatg tcgcgatctc ggcaaagttc
    21001 agtttttccg gtggagtgat acgaatgtaa tcgtgcccct cttctacttc cgcaccgact
    21061 ttacgcagtt ctgttgccat cgcaaacagg cgatcggttt ctttaacacg ccagttatag
    21121 atattgcgca gcgtggtggt gccttttgca aataacgccg ccgtggcaat ggtcatcgcc
    21181 gcatcgggaa tatggttcat atccatatca atagcgttca gttcaccacg cgtgcaggaa
    21241 atataatcat cgccccagca aatggtcgcg cccatttttt ccagcacatc agcaaagcga
    21301 atatcaccct gcatactgtt acgtccaata ccggtcactt ttacagtgcc gcctttgatt
    21361 gctgctgctg ccagaaagta agaagccgaa gatgcatcgc cttcgaccaa ataagtaccc
    21421 ggagactgat aagactgtcc gccttttacg acaaattgtt gatagtgctg attttcaatt
    21481 tcaacaccaa acgtcttcat cagattgagt gtgatgtcga tataaggttt agaaaccaga
    21541 tcgcctttaa tacgaatcac cgtatcttct ggcgcaagag gcgcagtcat taacagtgcg
    21601 gtgaggaatt ggctggaaac ggagccatca acgtcaacgt tgccgccagt aaagccgccc
    21661 tgtaaacgca acggcggata attttcttgt tccaggtaag tgatcttcgc cccgcccagg
    21721 cgcagcgcat ccaccagatg accaatcggg cgttctttca tacgcggctc accggtcagc
    21781 acaatatcat tgctacccag acaaagagct gccgccagcg gacgcattgc cgttccggcg
    21841 ttaccgagga acaactccag ggcaccttct gcgtgtaatg gaccgccgtt accgataatt
    21901 tcgcaacgcg tacgatcggc tgaaagcgta tagcttaccc ctaacgctgt taatgcattc
    21961 agcatatggc gcacgtcatc gctatccagc agattggtta atactgtttt gccgtgtgct
    22021 aatgccgcca gcaataaagc gcggttagaa acgctcttgg aaccgggcag attaatagtg
    22081 ccatcgacac gagcgatggg ttgtaacgtc agggattcca tgaactcaac tctctacaac
    22141 agaaataaaa accccacagg ctggctgtgg ggattaagca aaatttcggc attaaccgtg
    22201 acggcgttcg aactcaacca tgaagtctgt cagcgcttta acgccttcca gcggcatggc
    22261 gttataaata gaagcgcgca ttccgccgac cacacggtga cctttcagtg catgaaggcc
    22321 agcagcaaaa gactcttcaa ggaacaattt gtcaagcgca ctgtccgcca actggaacgg
    22381 cacgttcatc cgcgaacggt tagctttcgc cacgtcattg cggtagaaat cgctgttatc
    22441 aatcacccca tatagcagtt ctgctttttg ctgattgatt ttatccattt cagctacacc
    22501 gccgttcgct ttcagccatt taaagaccag accagataaa taccaggcaa atgtcggcgg
    22561 cgtgttaaac atggagccgt tatcgttgaa gatggaataa tccagtatcg acggacacgc
    22621 gatattcgct ttgcccagca aatcttcacg aacgatgacg attgtcaggc cagccgggcc
    22681 gatatttttc tgcgcgccag cgtaaatcac gccataacgg ctgacgtcaa tcggacggga
    22741 aagaatggtt gaagagaagt cggcggcgac caccacatct ttgccgaagt ctggcgtttc
    22801 gtcgatggcg ataccgtcga tggtttcatt cgggcagtaa tgcatataag cagcattatc
    22861 agagagttgc cattcacgca ttggcttaac cgcgcgcaga ccatcaacag tcactttggc
    22921 gtcaaagaca ttaggcgtgc agtatttttt cgcttcttta atggcacttg ccgcccagta
    22981 accggcatca acataatctg cggtggtttt atcaccgaga atattcagcg gtaccgcagc
    23041 aaactgaccg cgaccaccgc catggcagaa taataccttg tagttggagg ggacattaag
    23101 aagatcgcga aaatccttct cggcttcctc tgcaacctga atgaactctt tgccacggtg
    23161 actcacttcc atcaccgacg taccaagacc gttccagtcg cgcagttcct gttgagcctg
    23221 tttaagcacc tctgccggta gcattgccgg accagaacta aaattgaaga tttgagccat
    23281 ttcccctcac cacgttgcgt tgctattaac ccgcggtcaa tcgattgcga ccacaggtaa
    23341 tacattattc ctgtggctat cggttttatc attcagtgac acacatcgca atgagtaaaa
    23401 ccgttgaggg caaaaatgtg gcttgcatca cacaaaagaa tctctcggat tattagcaaa
    23461 aagccgatgt ttttactgtc aggtcacccg ttttgcccga cttgccttcg tccattctgg
    23521 atttgcacag cgcggacata attgtttttc ccccgctttc atatgaatga tttcactaca
    23581 acgaatacag gcatagtccg ccgactccgg caccactttg taacgctgcg tacaatcact
    23641 gggtaaaata agtaaacccg gtttcacctt gtcatcttca aagaaataga cactgatttg
    23701 cccgttggtt tcgagaatcg ccagacgtac ctgccccagc tgctccacgc cacgcaatcg
    23761 tagctccata aagaactcaa attccgtcat gttggagtta ttgagtttcg accaggccag
    23821 ctcgccatct tcaataataa caaccggctt accttccagt agatcttcca gtttttcact
    23881 gtgcgccatc aaccacatta ccaggcggta taacaacgcc agagtgataa agacgataag
    23941 taccggcacc atcggcacat catcataaaa cgccacatct cccgccgctg aacccagcgt
    24001 gagaatgatt aacacttcaa acagcgacat ctgtcgcaca ccgcgtcttc cggtcatttt
    24061 gaggaacaaa aaaaccagta caaaggtata aagactacgt agtgcaactt cgccaaggaa
    24121 atcaaaaggc actttatcaa atgccatacg gtggagatcg aacgccttca tttttatgct
    24181 cttatcgtgg tttattcgct aaaagcatag tcaagagttc atcggcatgg cggcggtgat
    24241 agcgcctttg agataaaacc cgtattattg cgcgctttcc gtacgactaa agtgattttc
    24301 atgacgcaaa catttatccc cggcaaagat gccgctctgg aagattccat cgctcgcttc
    24361 cagcaaaaac tttcagacct cggctttcag attgaagagg cctcctggct gaatcctgtg
    24421 cctaacgtct ggtctgtaca tattcgcgac aaagagtgcg cactgtgttt taccaacggt
    24481 aaaggcgcaa ccaaaaaagc ggcgctggct tctgcacttg gtgaatattt cgagcgtctc
    24541 tcaaccaact acttttttgc tgatttctgg ctgggcgaaa ccatcgccaa cggtccgttc
    24601 gtgcattatc ccaacgaaaa atggttccca ctgaccgaaa atgacgatgt gccagaagga
    24661 ctactcgatg accgtctgcg cgcgttttat gatccggaga atgaactgac cggcagcatg
    24721 ctgattgacc tacaatccgg taacgaagat cgtggtattt gcggcctgcc gtttacgcgt
    24781 cagtccgaca atcagaccgt ttatattccg atgaatatca ttggtaacct gtacgtctcc
    24841 aacggtatgt ccgcaggtaa tacccgcaac gaagcacgcg ttcagggatt gtctgaagtt
    24901 ttcgaacgct acgtgaaaaa ccgcattatt gctgaaagca tcagcctgcc agagatcccg
    24961 gcagacgtgc tggcgcgtta cccagcggtg gttgaagcga tcgaaacact ggaagcagaa
    25021 ggtttcccga tcttcgcata tgatggttcc cttggcggcc agtatccggt gatttgcgtg
    25081 gtactgttca atcctgctaa cggtacctgc tttgcctctt tcggtgcgca tcctgatttt
    25141 ggcgtagcac tggaacgtac cgtgaccgag ctgctgcaag gtcgtggcct gaaagatttg
    25201 gatgtgttta ctccgccaac cttcgatgat gaagaagtcg ctgaacatac caacctcgaa
    25261 acgcacttta tcgattccag cggtttaatc tcctgggacc tgttcaagca ggatgccgat
    25321 tatccgtttg tggactggaa tttctccggc accacggaag aagagtttgc tacgctgatg
    25381 gctatcttca acaaagaaga taaagaagtt tatattgccg attacgagca tctgggcgtt
    25441 tatgcttgcc gtattatcgt gcctggcatg tccgatattt atccggctga agatctgtgg
    25501 ctcgcgaata acagtatggg cagccattta cgtgaaacga ttctttcgct accaggcagc
    25561 gagtgggaaa aagaagatta cctgaacctc atcgagcaac tggatgaaga aggttttgat
    25621 gactttaccc gcgtgcgtga gctgttgggt ctggcgaccg ggtcggataa cggttggtac
    25681 accctgcgta tcggtgaatt aaaagccatg ctggcgctgg ctggtggcga tctggaacag
    25741 gctctggtct ggaccgaatg gacgatggag tttaactcat cagtatttag tccggaacgc
    25801 gccaactatt atcgctgcct gcaaacgttg ttattactgg cacaggaaga agatcgccag
    25861 ccgctgcaat atctgaatgc gtttgttcgc atgtacggcg cagatgccgt ggaagccgcc
    25921 agtgcggcaa tgagcggcga agcggcgttt tacggcctgc aaccggtaga tagcgatctg
    25981 cacgcgtttg ctgcacatca gtcgttgttg aaggcctacg aaaagctgca gcgcgccaaa
    26041 gcagcattct gggcaaaata aaatcaaata gcctacgcaa tgtaggctta atgattagtc
    26101 tgagttatat tacggggcgt ttttttaatg ccccgcttta catatatttg cattaataaa
    26161 ataattgtaa ttataaggtt aaatatcggt aatttgtatt taataaatac gatcgatatt
    26221 gttactttat tcgcctgatg ctccctttta attaactatt ttagcggagg atgcggaaaa
    26281 aattcaactc atttgttaat ttttaaaatt tatttttatt tggataatca aatatttact
    26341 ccgtatttgc ataaaaacaa tgcgagttac gggcctataa gccaggcgag atatgatcta
    26401 tatcaatttc tcatctataa tgctttgtta gtatctcgtc gccgacttaa taaagagaga
    26461 gttagtgtga aagctgacaa cccttttgat cttttacttc ctgctgcaat ggccaaagtg
    26521 gccgaagagg cgggtgtcta taaagcaacg aaacatccgc ttaagacttt ctatctggcg
    26581 attaccgccg gtgttttcat ctcaatcgca ttcgtcttct atatcacagc aaccactggc
    26641 acaggcacaa tgcccttcgg catggcaaaa ctggttggcg gcatttgctt ctctctgggg
    26701 ctgattcttt gtgttgtctg cggagccgat ctctttactt ccaccgtgtt gattgttgtt
    26761 gctaaggcga gtgggcgcat cacctggggt cagttggcga aaaactggct aaatgtctat
    26821 tttggcaacc tggtcggcgc actgctgttt gtacttttaa tgtggctttc cggcgagtat
    26881 atgaccgcaa atggtcaatg gggactaaac gtcctacaaa ccgccgacca caaagtgcac
    26941 catactttta ttgaggccgt ctgtcttggt atcctggcaa acctgatggt atgtctggca
    27001 gtatggatga gttattctgg ccgcagcctg atggacaaag cgttcattat ggtgctgccg
    27061 gtcgcgatgt ttgttgccag cggttttgag cacagtatcg caaacatgtt tatgatcccg
    27121 atgggtattg taatccgcga cttcgcatct ccggaattct ggaccgctgt cggttctgca
    27181 ccggaaaatt tttctcacct gaccgtgatg aacttcatca ctgataacct gatcccggtt
    27241 acgatcggta atattatcgg cggtggtttg ttggttgggt tgacatactg ggtcatttac
    27301 ctgcgtgaaa acgatcacca ttaatggttg tcgaagtacg cagtaaataa aaaatccact
    27361 taagaaggta ggtgttacat gtccgagctt aatgaaaagt tagccacagc ctgggaaggt
    27421 tttaccaaag gtgactggca gaatgaagta aacgtccgtg acttcattca gaaaaactac
    27481 actccgtacg agggtgacga gtccttccta gctggcgcta ctgaagcgac caccaccctg
    27541 tgggacaaag taatggaagg cgttaaactg gaaaaccgca ctcacgcgcc agttgacttt
    27601 gacaccgctg ttgcttccac catcacctct cacgacgctg gctacatcaa caagcagctt
    27661 gagaaaatcg ttggtctgca gactgaagct ccgctgaaac gtgctcttat cccgttcggt
    27721 ggtatcaaaa tgatcgaagg ttcctgcaaa gcgtacaacc gcgaactgga tccgatgatc
    27781 aaaaaaatct tcactgaata ccgtaaaact cacaaccagg gcgtgttcga cgtttacact
    27841 ccggacatcc tgcgttgccg taaatctggt gttctgaccg gtctgccaga tgcatatggc
    27901 cgtggccgta tcatcggtga ctaccgtcgc gttgcgctgt acggtatcga ctacctgatg
    27961 aaagacaaac tggcacagtt cacttctctg caggctgatc tggaaaacgg cgtaaacctg
    28021 gaacagacta tccgtctgcg cgaagaaatc gctgaacagc accgcgctct gggtcagatg
    28081 aaagaaatgg ctgcgaaata cggctacgac atctctggtc cggctaccaa cgctcaggaa
    28141 gctatccagt ggacttactt cggctacctg gctgctgtta agtctcagaa cggtgctgca
    28201 atgtccttcg gtcgtacctc caccttcctg gatgtgtaca tcgaacgtga cctgaaagct
    28261 ggcaagatca ccgaacaaga agcgcaggaa atggttgacc acctggtcat gaaactgcgt
    28321 atggttcgct tcctgcgtac tccggaatac gatgaactgt tctctggcga cccgatctgg
    28381 gcaaccgaat ctatcggtgg tatgggcctc gacggtcgta ccctggttac caaaaacagc
    28441 ttccgtttcc tgaacaccct gtacaccatg ggtccgtctc cggaaccgaa catgaccatt
    28501 ctgtggtctg aaaaactgcc gctgaacttc aagaaattcg ccgctaaagt gtccatcgac
    28561 acctcttctc tgcagtatga gaacgatgac ctgatgcgtc cggacttcaa caacgatgac
    28621 tatgctatcg cttgctgcgt aagcccgatg atcgttggta aacaaatgca gttcttcggt
    28681 gcgcgtgcaa acctggcgaa aaccatgctg tacgcaatca acggcggcgt tgacgaaaaa
    28741 ctgaaaatgc aggttggtcc gaagtctgaa ccgatcaaag gcgatctcct gaactacgat
    28801 gaagtgatgg agcgcatgga tcacttcatg gactggctgg ctaaacagta catcactgca
    28861 ctgaacatca tccactacat gcacgacaag tacagctacg aagcctctct gatggcgctg
    28921 cacgaccgtg acgttatccg caccatggcg tgtggtatcg ctggtctgtc cgttgctgct
    28981 gactccctgt ctgcaatcaa atatgcgaaa gttaaaccga ttcgtgacga agacggtctg
    29041 gctatcgact tcgaaatcga aggcgaatac ccgcagtttg gtaacaatga tccgcgtgta
    29101 gatgacctgg ctgttgacct ggtagaacgt ttcatgaaga aaattcagaa actgcacacc
    29161 taccgtgacg ctatcccgac tcagtctgtt ctgaccatca cttctaacgt tgtgtatggt
    29221 aagaaaactg gtaacacccc agacggtcgt cgtgctggcg cgccgttcgg accgggtgct
    29281 aacccgatgc acggtcgtga ccagaaaggt gctgtagcgt ctctgacttc cgttgctaaa
    29341 ctgccgtttg cttacgctaa agatggtatc tcctacacct tctctatcgt tccgaacgca
    29401 ctgggtaaag acgacgaagt tcgtaagacc aacctggctg gtctgatgga tggttacttc
    29461 caccacgaag catccatcga aggtggtcag cacctgaacg ttaacgtgat gaaccgtgaa
    29521 atgctgctcg acgcgatgga aaacccggaa aaatatccgc agctgaccat ccgtgtatct
    29581 ggctacgcag tacgtttcaa ctcgctgact aaagaacagc agcaggacgt tattactcgt
    29641 accttcactc aatctatgta attagatttg actgaaatcg tacagtaaaa agcgtacaat
    29701 aaaggctcca cgaaagtggg gcctttttta gcacgagagc cttttttgtc agctatctat
    29761 actttaaggt gactgccaaa acagactcga cgtagccttc gagctgcgca ccaacacggc
    29821 ctcagatggg ccacatctgg agaaacaccg caatgtcagt tattggtcgc attcactcct
    29881 ttgaatcctg tggaaccgta gacggcccgg gtattcgctt tatcaccttt ttccagggct
    29941 gcctgatgcg ctgcctgtat tgtcataacc gcgacacctg ggacacgcat ggcggtaaag
    30001 aagttaccgt tgaagatttg atgaaggaag tggtgaccta tcgccacttt atgaacgctt
    30061 ccggcggcgg cgttaccgca tccggcggtg aagcaatcct gcaagctgag tttgttcgtg
    30121 actggttccg cgcctgcaaa aaagaaggca ttcatacctg tctggacacc aacggttttg
    30181 ttcgtcgtta cgatccggtg attgatgaac tgctggaagt aaccgacctg gtaatgctcg
    30241 atctcaaaca gatgaacgac gagatccacc aaaatctggt tggagtttcc aaccaccgca
    30301 cgctggagtt cgccaaatat ctggcgaaca aaaatgtgaa ggtgtggatc cgctacgttg
    30361 ttgtcccagg ctggtctgac gatgacgatt cagcgcatcg cctcggtgaa tttacccgtg
    30421 atatgggcaa cgttgagaaa atcgaactcc tcccctacca cgagctgggc aaacacaaat
    30481 gggtggcaat gggtgaagag tacaaactcg acggtgttaa accacctaag aaagagacca
    30541 tggaacgcgt gaaaggcatt cttgagcagt acggtcataa ggtaatgttc taatttcccc
    30601 gtaaagcggc aactttattg agttgccgct tttttattcc gcgcttcatt ctgctacagt
    30661 gtttttaaca cattttatag tgcatctac
//
LOCUS       DAJTNA010000029        29340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTNA010000029 DAJTNA010000000
VERSION     DAJTNA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29340
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.029"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(312..2003)
                     /gene="fruA"
                     /locus_tag="OO161_001116"
     CDS             complement(312..2003)
                     /gene="fruA"
                     /locus_tag="OO161_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5462266.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2020..2958)
                     /gene="fruK"
                     /locus_tag="OO161_001117"
     CDS             complement(2020..2958)
                     /gene="fruK"
                     /locus_tag="OO161_001117"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5462267.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(2958..4088)
                     /gene="fruB"
                     /locus_tag="OO161_001118"
     CDS             complement(2958..4088)
                     /gene="fruB"
                     /locus_tag="OO161_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5462268.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4456..5637
                     /gene="setB"
                     /locus_tag="OO161_001119"
     CDS             4456..5637
                     /gene="setB"
                     /locus_tag="OO161_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5462269.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(5634..5888)
                     /locus_tag="OO161_001120"
     CDS             complement(5634..5888)
                     /locus_tag="OO161_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5462270.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6043..6615
                     /gene="yeiP"
                     /locus_tag="OO161_001121"
     CDS             6043..6615
                     /gene="yeiP"
                     /locus_tag="OO161_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5462271.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6838..8304
                     /locus_tag="OO161_001122"
     CDS             6838..8304
                     /locus_tag="OO161_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5462272.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8422..9408
                     /gene="yeiR"
                     /locus_tag="OO161_001123"
     CDS             8422..9408
                     /gene="yeiR"
                     /locus_tag="OO161_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5462273.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDHATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHIVKKGLA
                     ALSLPEHQSWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9447..10160
                     /gene="lpxT"
                     /locus_tag="OO161_001124"
     CDS             9447..10160
                     /gene="lpxT"
                     /locus_tag="OO161_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5462274.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVFFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10572..11138
                     /gene="mepS"
                     /locus_tag="OO161_001125"
     CDS             10572..11138
                     /gene="mepS"
                     /locus_tag="OO161_001125"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5462275.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11319..12875
                     /locus_tag="OO161_001126"
     CDS             11319..12875
                     /locus_tag="OO161_001126"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5462276.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            12957..14771
                     /locus_tag="OO161_001127"
     CDS             12957..14771
                     /locus_tag="OO161_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5462277.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYNGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14772..15866
                     /locus_tag="OO161_001128"
     CDS             14772..15866
                     /locus_tag="OO161_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5462278.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            15866..16891
                     /locus_tag="OO161_001129"
     CDS             15866..16891
                     /locus_tag="OO161_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5462279.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRNIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            16893..18482
                     /gene="yejF"
                     /locus_tag="OO161_001130"
     CDS             16893..18482
                     /gene="yejF"
                     /locus_tag="OO161_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5462280.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQATKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKQQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18486..18830)
                     /locus_tag="OO161_001131"
     CDS             complement(18486..18830)
                     /locus_tag="OO161_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462281.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALNDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19163..20353)
                     /gene="bcr"
                     /locus_tag="OO161_001132"
                     /pseudo
     CDS             complement(19163..20353)
                     /gene="bcr"
                     /locus_tag="OO161_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20381..21076)
                     /gene="rsuA"
                     /locus_tag="OO161_001133"
     CDS             complement(20381..21076)
                     /gene="rsuA"
                     /locus_tag="OO161_001133"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5462282.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21225..22985
                     /locus_tag="OO161_001134"
     CDS             21225..22985
                     /locus_tag="OO161_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5462283.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFHFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23110..23394
                     /gene="rplY"
                     /locus_tag="OO161_001135"
     CDS             23110..23394
                     /gene="rplY"
                     /locus_tag="OO161_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5462284.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKAAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23533..24540)
                     /gene="yejK"
                     /locus_tag="OO161_001136"
     CDS             complement(23533..24540)
                     /gene="yejK"
                     /locus_tag="OO161_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5462285.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24722..24949
                     /locus_tag="OO161_001137"
     CDS             24722..24949
                     /locus_tag="OO161_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5462286.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRLAIANSFARALQSSINEDKAH"
     gene            24969..26729
                     /gene="pbgA"
                     /locus_tag="OO161_001138"
     CDS             24969..26729
                     /gene="pbgA"
                     /locus_tag="OO161_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5462287.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26804..26880
                     /locus_tag="OO161_001139"
     tRNA            26804..26880
                     /locus_tag="OO161_001139"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26838..26840,aa:Pro,seq:ggg)
     gene            complement(26983..>29340)
                     /gene="yejO"
                     /locus_tag="OO161_001140"
     CDS             complement(26983..>29340)
                     /gene="yejO"
                     /locus_tag="OO161_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5462288.1"
                     /translation="NGGYQYIEMNGTAEYSVLNDGYQIVQMGGAANQTTLNNGVLQVY
                     GAANDPMIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGGAIKASTRVM
                     EAFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTATG
                     VDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAIDTIL
                     DEHVTMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANVWAGT
                     MVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSV
                     WAIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNTLSGN
                     GNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDAAFTL
                     GNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDTV
                     RERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESSTI
                     RGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGKM
                     SNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRADV
                     GNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNHVKVNDDGKFNNDVAG
                     TPGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT         7074 a         7693 c         7184 g         7389 t
ORIGIN      
        1 gttttacgta aagtaaatta ccgccagaga ggcaaaatgg gcagatttga gcaggctcac
       61 aaaatgcgat taaatgcctg aattacaaaa gcgaggaatt tgtgacggga tgcacagaac
      121 gggaacaata aaaaaagcag aaaataatct gcccttgagg aaataacctt agtatttgat
      181 gaacgtttca agtttctact gaaatataaa accatttaaa ttcataaaat tacattcaca
      241 aaccggtcat tctctttgcg ctcatcaaat gttacagggc aggaaatttc tgccctgtaa
      301 cacacctttt attacgctgc tttcgctact gcgtccactt ccggacgttt caggaaggca
      361 taggccaaac ccgccaccag cgtaccagca ataattgcta ctaggtaacc cagtaccggc
      421 gtaatagcgc cagggatcag cagaacaaac agaccaccgt gcggtgccat cagtttcgca
      481 ccaatcgcca ttgagattgc gccagtcagc gccccaccca cgatacagca cggcagcaca
      541 cgcatcggat cacgagcagc aaacggaatt gcaccttccg aaatgaagca cagtcccaat
      601 accagagcgg ctttgccacc ttcctgctgc gctttgtcga atttgcgacg cgccaccatt
      661 gttgccagac ccattgccag cggtggcacc atacctgccg ccataatcgc cgccatcggg
      721 ccatatgttt gagtactcag cagacccaca ccgaatgcgt acgctgcttt gtttaccgga
      781 ccgcccatgt cagtacacat catgccaccg aggatcgccc ccagcagaac cgcattcgca
      841 gtccccatgg tctgcagcca gtgagtcagc ccttcgagaa tgccagcaac tggtttaccg
      901 atcaggtaga tcatcgccag accgaccacc agactggaaa ttagcggaat gatcaggatc
      961 ggtttcagcg cctccatact ctgtggcagt ttcagttgcg tactgattaa cttcgcaatg
     1021 taaccagcca ggaagccagc aattataccg ccaataaagc cagaaccggt gctgaccgcc
     1081 agcataccgc caatcagacc cggcgtgagg cccggacgat cggcaatgga gaaggcaata
     1141 taacctgcca gtaccggcac catcagcgca aaggctgaac caccgccaat ctgcatcagc
     1201 gccgctgcca acgtacccgg ctctttaaac gcttcgatac caaaagcaaa agaaagcgcg
     1261 atacacagac cacctgcgac gaccatcggc agcatataag agacgcctgt cagcaagtga
     1321 cggtaagcgc ctgcactctc tttcttacct tcagtggtcg ccgtttgagc tttgcccgcc
     1381 ggttcatacg gcgttgcttc agcaaccgct ttatccagtt cctgcgcggt tttcttcagc
     1441 gccagaccgg tagaggtacg atacatcggt ttaccagcaa atttcgccag atccacttcg
     1501 atatctgccg ccacaatcac cagatccgct gctgcgactt cttcaggggt gattgcattg
     1561 cccgcgccaa cagaaccacg ggtttcaact ttcacccacc agccacgttt tttcgcttcg
     1621 gtttcaatgg cttcagccgc cataaaggtg tgtgctacgc cagtcgggca agcagtcacc
     1681 gcaactacgc gtttcggacc gctggcagca accggtgctg tcgcagcgac cggcgcagtg
     1741 taaggtttcg catggccttt ggcttcactc aggaacagct cagggtgcgc aactgcccgg
     1801 gaaatatcac ccagccagac atttttaccg ttcagcgcgc tgtcattcgg gatggaatca
     1861 ccgagaacaa tcgccatttc tgcgtcgttc ggattgtcga tgatttccag ttttgctttt
     1921 cgcgccgccg cgcccagcag ggtcttcgcc atataggcgc gtgcctgacc gagattagcg
     1981 tcaataatca gcagcgtttt cattatgcct ctcctgctgt cagttaaaag gttgtaagtc
     2041 gacgcgcgcc atcattgcgg ccaactgcgg acgatcggta atacccacat tgctttgact
     2101 taccgccagg gctgcaacag ctgtcgccag acgcagtgtg tgttcactgg attcacgcat
     2161 cagcaagcca taaatcaggc caccaaccat agaatcccct gcgccaacgg tgcttacgac
     2221 atcgactgac ggtggtttgg cgatccattc gccggaggca ttaacccaaa gcgcgccttc
     2281 ggcacccagt gaaataacaa catgcgcaat accttgttca cgtagcgcat gtgcagcttc
     2341 aatcacatct ttcatttcag gcagtttacg gcctgcccag atttccaact cgcggcggtt
     2401 aggtttcacc agccacggtg ccgctttcaa acctgctact aacgcttcac ggctactatc
     2461 aaagataatg caaggacact gactacgcag gcgagtcatc cagtcggtga acgcttccgg
     2521 gctgacgcct gacggtaagc ttccgctgac acagaccata tcgaactgac cgagccagct
     2581 cagagaatca gtcacaaagc gttcccagtc ggcgggggtg acttcaaaac ccgagaagtt
     2641 gaagtcggtc acttcgccgt ctttttccgt cagcttaacg ttaattcggg tgcgcccctg
     2701 tacaacctgg aaacggttgg caatgcccag ctcgctgaac agttgctgaa aaccatcctg
     2761 attgtcttta cccaggaagc cgccaacggt gacatcaatt cccaggtctt ttaatacttt
     2821 ggccacgttg atgcctttac ccgccgcatg cagaccggtg gttttcacca ggttcacttc
     2881 gccgcgttca atttccgggc agaaaccaac aaggtcataa gccggattaa gggtgatcgt
     2941 agcaacacgt ctgctcatta tgcgccctcc ccaagaccag cagcgatagc gtcgccaatg
     3001 gctttcagcg cctgttcagc atctgcaccc tgggcggtaa agcgtaggcg atgacctttc
     3061 ttaacgccaa gtgccacaac tttcatcaga ctacgtccgt ttgccggttt gccggtgcca
     3121 tcaaggtttg tcacggtaat atcactgtta aattgtttaa tggtattgac cagcatggta
     3181 cctggacgag catgcaggcc gtgttcattg cgcaccacaa actccgcgct taacacgtcg
     3241 tcggtcggcg catcatcgct ggtcagcagc gccagcaacg ttgccgcatc tgctttcagc
     3301 aagcggtcag ctttattgtc gagcaataaa tcagcgagac gcttaagaac cgcgatgggc
     3361 tgatcgtcat tcatcgccac actcaccagc atggctgccg tttcgccgtc cacagcaaaa
     3421 gcatttgccg cacggcttac cgcaatcgcg ctacgcagat tgccttcggc gctatcgctc
     3481 agccagatac cctgtccgag attcagcggt tgttcattga tggctttggt gacgaaagtg
     3541 gcgtcaactg cccccgcctc tttcagacgc gcagcgttca gcgcctgaag agtcagcaga
     3601 tcgctggcga cgatatccag cgtcagcatt tcgttgtcga gcttcagctg ctcactctgc
     3661 ttttcgccca tcagtaatgc gcgaagttct tctgctgttg ttgctgactt cagttgttca
     3721 gcaacggaat catcgctcag tacgtgggtc agctggcgta gcaggcccag atgttcatcc
     3781 gagctggcag caataccgat tgccacgtac gctacctgac cgtcacccca ggtgacgcct
     3841 tccgggaact gaaatacctg aacgccggtt ttcagcacct gatcgcgggt gtcggtagtg
     3901 ccgtgtggaa tagcaatacc attgccgagg aacgttgagg tttgctgttc gcgcgccagc
     3961 atgccattga cgtagccttc tgctacatta ccggcctgca ccagcgccgc agcgacctgg
     4021 cgaatcgcct cttctttgtc tccggccttt tcgcccggat ggatgtcctg tacggataac
     4081 tggaacatag ttctcctctc ttgctgaatt gaaacgattc ggcctctatg agaaaaaaag
     4141 cgccaacctg gcttagggtt aaagacaaga tcgcgctgaa acgtttcaag aaagcataat
     4201 acttctgttt cagcacgcaa ggaaagccgt aaagttatga gcaaaagttt gatgtgctgc
     4261 acattttttt gtcatttttc ctaattgctg atgggaaaat ccgttatcag caatttcatt
     4321 tcagcagcgt cagcagccaa tcagcaacag tgaaatggca ttttgattat tcgtggcgct
     4381 tttgatttcg ctgtctgttt attttctgac aagcagcgta aactccgcgt cttcctcttc
     4441 cagtgataga cctgcatgca taactccccc gcagtctcca gcgcgaaatc gtttgacctg
     4501 acctcgacgg cgtttttaat cgttgccttt ctcaccggta ttgcgggtgc tctgcaaacc
     4561 ccgacactca gtattttcct taccgatgaa gtacatgccc gtccggcgat ggtgggattc
     4621 ttctttaccg gcagcgctgt cattgggatt ctggtaagtc agtttctcgc tgggcgctct
     4681 gataagcgcg gcgatcgcaa atcgctgatt gtcttttgct gcctgttagg cgtgctggcc
     4741 tgcacccttt ttgcctggaa tcgcaactac tttgttttgc tattcgttgg cgtctttctt
     4801 agcagctttg gctcgaccgc taacccgcaa atgtttgccc ttgcccgtga acatgccgac
     4861 aaaaccggac gtgaggcggt gatgttcagc tcttttttac gcgctcaggt ttcactggca
     4921 tgggtcattg gcccaccgct ggcttatgcg ttagcgatgg gtttcagctt tacggtaatg
     4981 tatctgagcg cagcggtagc gtttattgtt tgcggcgtga tggtgtggct gtttttaccg
     5041 tcgatgcaaa aagagcttcc gctggcgacc ggcacggttg aagcgccgcg ccgtaaccgt
     5101 cgcgatacgc tactgctgtt tgtcatttgt acattgatgt ggggctcgaa cagcctgtac
     5161 atcatcaaca tgccgctatt tattatcaac gaactgcatc ttcccgagaa actggccggt
     5221 gtgatgatgg ggaccgccgc cgggctggaa atcccgacga tgttgattgc cggatatttc
     5281 gccaaacgtc tgggtaagcg tttcttaatg cgcgttgctg ccgtgggtgg cgtctgtttt
     5341 tacgcaggaa tgctgatggc gcattcacct gccattctgt tgggcttgca gctgctaaat
     5401 gctattttta ttggcattct gggcggcatc gggatgctct attttcagga tttgatgccc
     5461 ggtcaggcag gttcagccac cacgctctat accaacacgt cgcgcgtggg ctggatcatc
     5521 gcaggatcag tggcgggcat cgtcgccgag atctggaatt atcacgctgt gttctggttt
     5581 gcgatggtga tgattatcgc cactctgttt tgcttactgc ggattaaaga tgtttaagac
     5641 gcggtcagca tttccagatc gataagccag gtcatggcct gctgacgcga gttaccgcac
     5701 atttcagcgc tggcttgtaa gcctgcacag actttcggtc gcagtggcga ggtgaaaatt
     5761 ttacagcgtt gctgttcatc aagctgaatg cagggcgtat tggcgggctt gccatctggc
     5821 ataccgggaa tagggctgaa aattgaaggg gcagtgcaac acgccccgca acccggacgg
     5881 cattccatac gttgttctct taaggttaac caccggattg atgagcgcgc acagtaccac
     5941 cttttttgca ccagcaaaag tgcgaatacc acttgcccga aaggcccgtc gctagtactt
     6001 tgtcgcgata tttttgacat tttcgactac aggaattttt cgatgccaag agcgaacgaa
     6061 attaaaaaag gtatggtact gaattacaac ggcaaactgc tgctggttaa ggatattgat
     6121 attcagtcgc ccactgcccg cggtgccgct acgctgtaca aaatgcgttt ttctgatgtc
     6181 cgtaccgggc tgaaagtaga agagcgtttc aaaggtgatg atatcgttga caccgtgacg
     6241 ctgacccgcc gttacgttga cttctcctat gtcgatggca acgaatatgt ctttatggat
     6301 aaagaagact ataccccgta taccttcacc aaagatcaga ttgaagaaga gttgctgttt
     6361 atgccagagg gcggcatgcc ggacatgcag gtgctgacct gggacggtca actgctggcg
     6421 cttgagctgc cgcagaccgt tgatctggaa atcgttgaaa ccgcaccagg catcaaaggg
     6481 gcatctgcca gcgcccgtaa caaaccggcg acattgagca ctggtctggt gattcaggta
     6541 ccagaatact taagcccggg cgaaaaaatt cgtatccata tcgaagaacg ccgttatatg
     6601 gggcgtgctg actaacttca gccgcatgca gaaaagggat agctcaggct gtcccttttt
     6661 taatttatta taccaatctt ctattttgcg cttcctgaac accttacgcc ctcaattttc
     6721 actcgttgat tgatcgccct cacacttcat cgcattaaca atccagacca atttcaattg
     6781 ctgtcatata actttacact gtcattgtta atttatcgtt actaagacgt gactcctatg
     6841 aagacaattg cctccgttac gctcccgcat catgtacacg ctccacgcta cgatcgccag
     6901 cagttgcaat cacgtatcgt tcattttggc tttggcgcct ttcaccgcgc tcatcaggcg
     6961 ttactgaccg atcgtgtgct gaatgcccag ggcggcgact gggggatctg tgaaatcagc
     7021 ttgttcagcg gtgatcaact gatgagccag ctccgcgcac agaaccattt atataccgtg
     7081 ctggagaaag gtgcggacgg caatcaggtg ataattgtcg gtgccgttca cgaatgcctt
     7141 aatgcaaaac tggattcctt agcggcaatt attgagaaat tttgcgagcc acaggtggca
     7201 attgtttccc tgacgattac cgaaaaaggc tattgtattg acccggccac cggtgcactc
     7261 gacaccagta atccgcggat tattcacgat ctacaaaccc ctgaagaacc tcactccgca
     7321 ccgggtattc tcgtcgaagc actgaaacgc cgccgtgagc gcggccttac accgtttacc
     7381 gtgctctcct gcgacaatat tcccgacaat ggccatgtgg tgaaaaacgc ggtgctggga
     7441 atggcagaaa aacgttcgcc agaactcgcc gggtggataa aagaacacgt cagttttccg
     7501 ggaaccatgg tcgaccgcat tgttccggct gcaaccgacg aatcactggc agaaatcagc
     7561 cagcatctgg gggtaaatga tccctgcgcg attagctgcg aaccgtttat ccagtgggtg
     7621 gtggaagata acttcgtcgc tgggcgtcct gcctgggaag tcgcaggtgt acaaatggtg
     7681 aatgatgtcc tgccatggga agagatgaaa ctgcgtatgc ttaatggcag ccactctttt
     7741 ctcgcttatc tgggttacct ttcaggattc gcccatatca gtgattgcat gcaggatcgc
     7801 gcatttcgcc atgccgccag aacattaatg ctgaatgagc aagcgccgac actacaaatt
     7861 aaagatgtcg atttaacaca atatgcggac aagttaattg cacgttttgc taatccggcg
     7921 ctgaaacata agacctggca aatcgcaatg gatggtagcc agaaattacc gcaacgtatg
     7981 ctggcaggta ttcgcataca tctggggcgc gaaacggact ggtcgttgct ggcattaggc
     8041 gttgctggct ggatgcgtta cgtcagcggc gttgatgatg ccggaaatgc cattgatgtt
     8101 cgcgatccgc ttagcgataa aattcgcgaa cttgttgcag gcagcagcag tgaacaacgc
     8161 gtaaccgccc tgctttccct gcgtgaagtt ttcggtgatg atctgccaga taacccgcat
     8221 tttgtgcagg ccatcgaaca agcctggcaa caaatcgtac aattcggcgc acatcaggcg
     8281 ctattaaaca ccctcaaaat ttaacgattt ctgcggttaa agcggatgaa gctcaccttc
     8341 gtccgctctc cccttctctt ttctgccttt tttagccagg attaacgctc agttaactta
     8401 ccagagtatt gcggagccgt tgtgaccagg accaacctca tcaccggttt tctcggcagc
     8461 gggaaaacca cgtcgattct tcatctgtta gcccataaag atcccaacga aaaatgggcg
     8521 gtactggtta atgaatttgg ggaagtcgga attgatggtg ctttgctcgc cgatagcggc
     8581 gcattgctga aagagatccc cggcggctgc atgtgctgcg ttaatggttt acccatgcag
     8641 gtagggttga ataccttact gcgtcaggga aaaccagacc gcttgttgat agagccgacc
     8701 gggctgggcc atccgaaaca gatcctcgat ctgttaaccg caccagtcta tgaaccgtgg
     8761 atagatctgc gcgccacctt gtgcattctc gatccacgct tgctgctgga cgaaaaaagc
     8821 gccagcaatg aaaacttccg tgaccagctg gctgccgcag acatcattgt cgccaataaa
     8881 tccgaccatg cgacgcccga aagtgagcaa gcgctacagc gttggtggca gcaaaatggt
     8941 ggcgatcgac aattaattca cagtgagcat gggaaagttg acggtcatct tctggatttg
     9001 ccgcgtcgca atttagccga gttgcccgcc agcgccgcgc attctcatca gcatatcgtg
     9061 aaaaaagggt tagcagcgtt aagcctgcca gagcatcaaa gctggcgtcg cagtctgaac
     9121 agcgggcaag gatatcaggc ctgcggctgg atattcgacg ctgatacggt attcgacacc
     9181 attggcattc tggaatgggc gcgacttgca ccggtggaac gcgtcaaagg cgtgctgcgt
     9241 attcccgaag ggctggtacg aatcaaccgt cagggcgatg acctgcacat tgaaacgcaa
     9301 aacgttgcgc caccggacag ccgtattgag ctgatttcca gcagcgaagc tgactggaat
     9361 gccttgcaga gcgcgctgtt gaagcttcgt ttagcgacta ccgcgtaagg ttgcctgcgt
     9421 ttttcagtaa gataattaga gaatatatga ttaaaaattt gccgcaaata gtgttgttga
     9481 atattgtcgg cctcgcgctg tttctttcct ggtatattcc cgttaatcat ggattatggt
     9541 tgccgattga tgcggatatt ttttatttct ttaatcagaa actggtcgaa agtaaggcct
     9601 ttttatggct ggttgcattg accaacaatc gcgctttcga cggttgttct ctgctggcga
     9661 tgggaatgtt gatgctgagt ttctggctga aagaaaacgc cccaggcaga cgacgtatcg
     9721 tgattattgg tctggtaatg ctattaactg cagtggtatt aaaccagctg ggtcaggcat
     9781 taattcctgt aaaacgggcc agcccaacat tgacgtttac taatattaac cgcgtcagcg
     9841 aactgctctc tgtccccacg aaagatgcct cacgagatag ctttcccggc gatcacggca
     9901 tgatgctgct tattttttcg gcattcatgt ggcgttattt cggtaaagtt gcaggtctta
     9961 tcgcccttat tatttttgtg ttttttgcgt ttcccagagt aatgattggc gcacactggt
    10021 ttactgacat cattgtcggt tcgatgaccg tgatattgat cggtttgccc tgggtgttgc
    10081 tgacgccatt aagtgatcga ttaatcacct tttttgacaa atcactaccg ggaaaaaaca
    10141 aacatttcca aaacaaataa ctcacagtaa ttaacatcat caggtttatt tttatagtga
    10201 ggataatcct gatgatgcgc accgtgcttt catctatcga acgcaaaaat cattctctaa
    10261 gtaaatgaat ggattgcatg cgtttcactc aatcgtactt taattgacca accccgctta
    10321 ttaactttct gtatcacttt ttcttataaa aaatcatgta aaaccgctcg ccaagaccgc
    10381 accaatcggg taatctcgaa ctcgttttgc ctcggcggta gattatcctc acagcatata
    10441 attttgtgcg ttagtccaca gatttggcct taaggaattg tttcaacatg cccaggtaat
    10501 tagtctcgtg tcgcttggca tttttttata acgatatttg tcgttaagga cttcaaggga
    10561 aaacaaacaa catggtcaaa tctcaaccga ttttgagata tatcttgcgc gggattcccg
    10621 cgattgcagt agcggttctg ctttctgcat gtagtgcaaa taacaccgca aagaatatgc
    10681 atcctgagac acgtgcagtg ggtagtgaaa catcatcact gcaagcttct caggatgaat
    10741 ttgaaaacct ggttcgtaat gtcgacgtaa aatcgcgaat tatggatcag tatgctgact
    10801 ggaaaggcgt acgttatcgt ctgggcggca gcactaaaaa aggtatcgat tgttctggtt
    10861 tcgtacagcg tacattccgt gagcaatttg gcttagaact tccgcgttcg acttacgaac
    10921 agcaggaaat gggtaaatct gtttcccgca gtaatttgcg tacgggtgat ttagttctgt
    10981 tccgtgccgg ttcaacggga cgccatgtcg gtatttatat cggcaacaat cagtttgtcc
    11041 atgcttccac cagcagtggt gttattattt ccagcatgaa tgaaccgtac tggaagaagc
    11101 gttacaacga agcacgccga gttctcagcc gcagctaata aaccgtttgg atgcaatccc
    11161 ttggctatcc tgacgagtta actgaaagca ctgcttaggc agtgcttttt tgttttcatt
    11221 catcagagaa aatgatgttt ccgcgtcttg atccaggcta tagtccggtc attgttatct
    11281 tttaaatgtt gtcgtaattt caggaaatta acggaatcat gttcatacgc gctcccaatt
    11341 ctggacgtaa gctcctgctt acctgcattg tagcaggcgt gatgattgcg atactggtta
    11401 gctgccttca gtttttagtg gcctggcata agcacgaagt caaatacgac acactgatta
    11461 tcgacgtaca aaagtatctc gatacctatt ttgccgacct gaaatccact actgaccggc
    11521 ttcagccgct gaccttagat acctgccagc aggctaaccc cgaactgacc gctcgcgcgg
    11581 cgtttagcat gaatgtccgt acgtttgtgc tggtgaaaga taaaaaaaca ttctgttcat
    11641 ctgcgactgg tgagatggac attccactca atgaattgat tccggcgctc gacattaata
    11701 aaaacgtcga tatggcgatc ttacccggca cgccgatggt gccgaacaaa cccgcaatcg
    11761 tcatctggta tcgcaaccct ttgctgaaaa atagcggtgt gtttgccgct ctgaatctca
    11821 acctgacgcc ttctctcttt tatagttcac ggcaggaaga ttacgatggc gtcgccctca
    11881 ttattggcaa tactgcgcta tctacctttt cttcacgttt gatgaacgtt aatgaattaa
    11941 ccgacatgcc ggtccgtgaa actaaaattg cgggcattcc tctgaccgtt cggctttatg
    12001 cggatgactg gacatggaac gatgtgtggt acgcattttt actgggcggc atgagtggaa
    12061 ctgtcgttgg cctgctctgc tattacctga tgagcgtacg tatgcgcccc ggcagagaaa
    12121 tcatgaccgc catcaagcgc gaacaatttt acgtggcgta tcaaccggtg gtggatacac
    12181 aagctttgcg agtaacgggc ctggaagtac tgctacgctg gcggcatcct gtcgcgggag
    12241 aaattccccc ggatgccttc attaactttg ccgaatcgca aaagatgatt gtgccgctga
    12301 ctcagcacct gtttgagtta attgcccgcg atgccgcaga attagaaaaa gtgctgccag
    12361 taggcgtcaa atttggtatt aacattgcgc cggaccatct gcacagcgaa agctttaaag
    12421 cagatatcca gaaactgctc acttccctgc ccgcacacca tttccagatt gtgctggaaa
    12481 ttaccgagcg cgatatgctg aaagagcaag aagccacaca actcttcgcc tggctgcact
    12541 cggtcggcgt agaaattgct attgatgact tcggcaccgg gcacagcgcg cttatctatc
    12601 ttgagcgttt tacgctcgat tatctgaaaa ttgaccgtgg atttatcaac gccatcggta
    12661 cggaaacgat cacttcaccc gtacttgacg cggtgctgac gctggcgaaa cgcctcaata
    12721 tgctgacggt tgctgaaggg gtcgaaacgc cggaacaggc gcgatggcta agcgaacgcg
    12781 gcgttaattt catgcaaggc tactggatta gtcgcccgtt accgctggac gattttgttc
    12841 gctggctgaa gaaaccgtat acgccgcagt ggtaaggtgt gcttacgtcc cttattattc
    12901 atagtgaaag catgccggat tgcggctaat gatgagtaaa aggaaatccg ttgcagatga
    12961 ttgtgcgcat actgctgctg tttatcgccc tgttcacctt cggtgcgcag gcgcaggcta
    13021 tcaaggaaag ctatgccttt gccgtgctgg gcgaaccccg gtacgcattt aatttcaacc
    13081 attttgatta tgtgaacccc gccgcgccaa aaggtgggca gataacgttg tcagccctcg
    13141 gtaccttcga taatttcaac cgctatgcac tgcgcggtaa cccgggcgca cgcaccgagc
    13201 agttgtacga cacgctattt acgacttccg atgacgaacc aggcagttat tacccgctga
    13261 ttgctgaaag tgcacgctat gctgacgatt attcctgggt ggaggtcgct attaatccgc
    13321 gcgcccgttt tcatgatggt tcgcccatta ctgcccgcga tgtagagttt acttttcaaa
    13381 aatttatgac cgaaggcgtg ccgcaatttc gtctggtcta caacggcacc accgtcaaag
    13441 ccattgcacc gttaaccgtg cgcattgagt tagctaaacc cggcaaagaa gatatgctga
    13501 gtctgttttc gctgccggta tttccagaaa agtactggaa ggatcacaaa cttagcgacc
    13561 cgctcgccac gcctccgctt gccagtggtc cgtaccgcgt tacgtcctgg aaaatggggc
    13621 aaaatattgt ctattcccgt gtgaaagatt actgggcagc aaacttaccg gtaaaccgtg
    13681 gacgctggaa tttcgacacc attcgctacg attattacct cgatgataat gtcgcctttg
    13741 aagcgtttaa agcaggtgcc tttgatttgc gtatggaaaa cgacgctaaa aactgggcca
    13801 cgcgttatac tggtaaaaat ttcgataaaa aatacatcat caaagatgag caaaagaacg
    13861 aatcagccca ggatacgcgc tggctggcgt ttaatatcca acgtccggta ttcagcgatc
    13921 gccgggtccg ggaagcaatc actctcgcct ttgactttga atggatgaac aaggcgttgt
    13981 tttacaacgc ctggagtcgc acgaacagtt attttcagaa taccgaatac gcggccagaa
    14041 attaccccga cgccgcggag ctggtgcttc tggcaccaat gaaaaaagat ctaccgccag
    14101 aagtcttcac acaaatctac cagccgccgg tatctaaagg cgatggctac gatcgtgaca
    14161 acctgttaaa agccgacaca cttctcaacg aagcgggctg ggtgctgaag ggtcagcaac
    14221 gcgttaatgc cacaacgggt cagccactca gctttgaatt attgcttccc gcaagcagca
    14281 atagtcagtg ggtattgccg ttccagcaca gcctgcaacg gctgggtatc aacatggaca
    14341 ttcgcaaggt ggataactcg caaatcacca accgcatgcg cagtcgcgac tatgacatga
    14401 tgccgcgcct atggcgggcg atgccgtggc ccagttccga tttacagatt tcctggtcat
    14461 cggaatatat caattccact tataatgccc ccggcgtgca aagcccggtt atcgactcac
    14521 tgatcaacca aattattgcc gcgcagggaa ataaagaaaa attattgccg ttggggcgag
    14581 cactggatcg cgtattaacg tggaattatt acatgctgcc aatgtggtac atggcggaag
    14641 accgtctcgc ctggtgggat aaattctccc aacccgctgt acgccctgtt tacagcctgg
    14701 gtatcgatac ctggtggtat gacgttaata aagcggccaa actgccgtca gccaggcaac
    14761 agggagagta gatgggcgct tacctgattc gccgtctgtt gctggtgatc ccaacattat
    14821 gggcgattat caccatcaac tttttcatcg tgcaaattgc gcctggcggt ccggtcgacc
    14881 aggccatcgc cgccattgag tttggtaatg ccggagtatt acccggcgca ggcggtgaag
    14941 gtgttcgtgc cagccatgcg caaacgggtg tcggcaatat cagcgacagt aattaccgtg
    15001 gcggacgcgg attagatcca gaagtgatcg ctgagatcac tcatcgctac ggttttgata
    15061 agccgatcca cgaacgttac ttcaaaatgc tctgggacta catccgcttt gattttggtg
    15121 atagcctgtt tcgcagcgcc tcggtgctga cgctgattaa agacagtctg ccggtttcca
    15181 tcaccctcgg attgtggagc acgctgatta tctatctggt gtcgattccg ttaggcattc
    15241 gcaaagctgt tcataatggg agccgctttg acgtctggag tagcgcattt atcattatcg
    15301 gctacgccat tccggccttt ttgtttgcca tcctgctgat tgtcttcttc gcgggcggca
    15361 gctatttcga cctgttccct ctacgcggcc tggtttccgc taactttgat tcgctgccgt
    15421 ggtatcagaa aatcaccgat tatctgtggc atatcacgct gccggtgctg gcgacggtga
    15481 ttggtggctt tgcggcgctg accatactga caaaaaactc attccttgat gaagtgcgca
    15541 agcaatacgt ggtgaccgcg cgtgcgaaag gggtaagtga aaaaaatatt ctctggaaac
    15601 atgtgttccg caacgccatg ctgctggtga ttgccggttt tccggcgacg tttatcagca
    15661 tgttttttac cggctcgctg ctgattgagg tgatgttttc actcaatggt ctgggcttac
    15721 tgggctacga agcgaccgtc tcgcgcgatt atcctgtaat gtttggtacc ttgtatattt
    15781 tcaccctgat tggcctgctg ctgaatattg tcagtgatat cagctatacg ctggttgatc
    15841 cgcgtattga ttttgaggga cgttaatgtc gcgactcagc cccgtcaatc aggcccgttg
    15901 ggcgcgtttt cgtcataacc gtcgcggcta ctggtcgtta tggattttcc tcgtcttgtt
    15961 tggtttgagt ttgtgttctg agcttatcgc caacgataaa ccgttgctgg tacgttatga
    16021 cggcagttgg tatttcccgt tgttgaaaaa ctacagcgaa agcgattttg gcggcccgct
    16081 ggcaagtcag gctgattatc aggacccgtg gctgaaacaa cggctggaaa ataacggctg
    16141 ggtactgtgg gcaccgattc gctttggtgc taccagtatc aactttgcta ccgataagcc
    16201 cttcccttct ccaccctccc ggcaaaactg gctgggaacg gatgccaacg gcggcgatgt
    16261 gctggcacgc attctctatg gcacgcggat ctcggttctg tttggcctga tgctgactct
    16321 ctgttccagc gtgatgggcg tgctggcggg ggcgctacaa ggctattacg gcggtaaagt
    16381 cgatctctgg ggacaacgct ttattgaagt atggtcgggg atgccgacgc tgtttttgat
    16441 tattttactt tccagcgtcg tacagcctaa cttctggtgg ctgctggcaa ttactgtctt
    16501 gtttggctgg atgagtctgg tcggcgtggt gcgggcggag tttttacgta ctcgtaattt
    16561 cgactacatt cgtgcggcgc aggcgcttgg cgtcagcgat cgcaatatca tcctgcgtca
    16621 tatgttgcct aatgccatgg tcgcgaccct caccttttta ccgtttattt tatgtagttc
    16681 gatcaccacc ctgacctcac tcgatttcct cggcttcggt ctgccgctcg gttcaccgtc
    16741 actcggtgaa ctgctgttac aagggaaaaa taaccttcag gctccgtggc ttgggatcac
    16801 cgccttcttg tcggtggcga tattattgtc tttgctgatc tttattggtg aagccgtccg
    16861 cgacgcattt gatcctaata aggcggtgta gcatgacgca aactctgtta gcgattgaag
    16921 atttgtcggt gggttttcgc catcagcaaa ccgtacgtac agtagtcaat gatgtttcac
    16981 tacagattga ggctggcgaa acgctggcgc tggtgggtga gtcaggttca ggcaaaagcg
    17041 ttaccgcgct gtcaatttta cgcctgctcc cttccccgcc ggttgaatat ctctccggcg
    17101 atattcgttt tcgtggcgaa tcgctgcttc atgccagcga tcaaacgttg cgcggtgtac
    17161 gcggtaataa gatcgccatg atttttcaag agccgatggt gtcgttaaat ccattgcata
    17221 ccctagaaaa acagctttat gaagtgcttt cactccaccg cgggatgcgt cgggaagcgg
    17281 ctcgaggcga aattcttaac tgccttgatc gcgttggtat ccgccaggcg acaaaacggc
    17341 tgacagatta tccgcatcag ctctccggcg gcgaacgcca gcgggtgatg attgcgatgg
    17401 cgctgttaac gcgaccggaa ttattaattg ccgatgaacc gaccaccgca ctggacgtct
    17461 ctgtccaggc gcagatttta cagctgttgc gcgaactgca aggcgagctg aatatgggca
    17521 tgctgtttat tactcataac ctcagcattg tcagaaaact ggcccaccgc gtggcggtaa
    17581 tgcaaaacgg tcgctgtgtc gagcaaaatt acgccgctac gctatttgcc tcacccactc
    17641 atccttacac acaaaagcta ctcaacagtg aaccgtcagg cgatccagtg ccgttgccag
    17701 aacctgcctc aacgttgctg gatgttgaac agcttcaggt tgccttcccc attcgcaaag
    17761 ggattttgaa gcgcattgtg gatcataatg tggtggtgaa aaacatcagt tttacgctac
    17821 gagcgggtga aacactgggt ttagtgggcg agtccggttc cgggaaaagt acgacgggac
    17881 tggcgctgct gcgactgatt aattctcagg gcagcatcat ctttgacggt cagccactgc
    17941 aaaatttaaa tcgccgccag ctgttaccta ttcgtcatcg cattcaggtg gtatttcagg
    18001 atccaaactc ctcgctcaac ccacgactca acgttttgca gattattgag gaaggcttac
    18061 gggttcacca gccgacgctt tctgccgcac aacgcgaaca acaagtgata gccgtgatgc
    18121 atgaagtggg attagatcct gaaacacgcc accgttatcc ggcggagttc tctggtggtc
    18181 agcgacaacg tattgcaatt gccagggcat taattcttaa gccctcgctg atcatacttg
    18241 atgaaccgac atcatcactc gacaaaacgg tacaggcgca aatattgacg ctattgaaat
    18301 cattgcaaca aaagcagcaa ctggcctatt tgtttatcag ccacgatttg cacgttgtcc
    18361 gcgcgttatg tcatcaggtt atcgtactgc gacaagggga agtagtggaa caaggaccgt
    18421 gcgcgcgcgt gtttgccgca ccgcagcagg agtatacgcg tcagctactg gcgttgagct
    18481 gacgcttaaa aaggattgta gtttgaaaag ggttcggcaa ttgccacacc aaaatttttc
    18541 aatcggcatg ttgcagcaaa ctcgtcctgg cgatttacaa acaggcacgg ctcaccttca
    18601 cactccagca ccgaacacgg aacttcgatg tcgtttaacg cctggcttag tttgtacatc
    18661 accgaccatg cattatcacc atccgggcta agcaatttaa gcgccaccag gctgttgtca
    18721 ccgcacggtc cattttgcgg aatcggttca accttcgaac ctgcgaaacc agcagaccag
    18781 cgaaagttct gcggtagacg atggacgatt gagagttgta atgaagtcac ttattttccc
    18841 cggaagcaca tttacttcac aatttgttta catcaatttt aacacatcat caacaatccg
    18901 tctttataca gatcgtaaag attgatgcct cgcctgcgtg gcctctatgg ctctgattta
    18961 agtaatatct gcgggcgtgg tcacgtttgc ggggctatat gtacccgttt ctgcgatcta
    19021 actcaacctt tttaactaca atgatgtgac tttttacata aattgatttt acataaaata
    19081 aacatatatc gggggaatga tagatttgtg gttgacatgc atcaacaaag gaagcctttt
    19141 agcttcctcg ttgtgcaata gatcaccgtt ttttcggtcg actggcgtac agacagaaga
    19201 gaatggagct gcttgcgcag aatgcaattg accaaatcat cggccatgca gagttaaagg
    19261 tcgcaagaga aagcaatgcg acaacaattg cccctatgcc aaaacggaag gttcctgcca
    19321 gcgaagatgc cgttcccgcc atatggggaa actcatcaag aatgaccgcc atcgcattgg
    19381 atgacaccat cgacacacag cccacaaacg ccgcaacgcc aaccaccagc gatcagaatc
    19441 ccagccccag cagcgcactg atgaccatcc acgctgccat aataaattgt atccacaacc
    19501 ccgagcggaa catatttaac gcgccaatgc ggcgaacgaa gcggctgtta aagatggtca
    19561 tcacgaacag gaaaacaatg tttagtgcaa agtaataacc aaagttttct ggcgcgacgt
    19621 ggttaatttc aatgtagaca aacggtccgg cgctcaggaa cgagaacatt ccggcaaagc
    19681 tgaaaccgct ggccagcata taactcagga cacgcttatg acggaacagc gccgcaaagt
    19741 taccaatagt agtacgaatg tgaaaaggct ggcgacgctc cggtggtaag gtttctttga
    19801 tcagaaagaa gatcattgcc gaagccagaa tcgccgccac ggcgaggatc cagaagatgt
    19861 aatgccagct cagccacacc agcacccagc cgccaactat cggtgccatc agcggtgcaa
    19921 tggtcgtcac cagcatgaca aacgacatca tccgcgagaa ctcttctttc gggtagatat
    19981 cacgcatcaa agcgttgata accatgctgg ccgcagccgc agccagcccg tggaaaaaac
    20041 gcatcacaat cagctgatcg atggtttgtg ccaacgcaca cgccaccgcg gcggcggcaa
    20101 acaccagcgt accgccgagc accaccggct tacgcccgaa gctgtctacc atcggcccgt
    20161 agattaactg ccccaacaca aagcccagaa tataagtact gagggtcatc tgcgtactgc
    20221 ccgccggtac gccaaactgc gctgaaatta ccggtagcgc gggcagatac atatcaatcg
    20281 acagcggcat caacatggcc agcaggccaa ggataaaaac aatagcaaac gacgaatgct
    20341 gtcgggtggt cacaacgggc tcctgaaagt cattgaaaag ttagacgacg ctggcaattt
    20401 cttcttcagt taacggacga tattcaccgg gggctaaatc agcatccagc gtaataccgc
    20461 caatacgttc acgatgcagc tcaaccacgt ggttacccac ggcggcgaac atgcgtttca
    20521 cctgatgata acgcccttcg ctgatggtca gacgaacctg cgttggggta atcacttcca
    20581 gcaccgcagg cttagtgaga tctttttcgt tatgcagctg cacgccttta gcaaattgct
    20641 ctgccgtatc gtcagctaca ggtgattcca gtgtcaccag ataggtcttc tcgcaatgat
    20701 ggcgcggaga agtaatgcgg tgcgaccact gaccatcatc agtcatcagc accagaccgg
    20761 tggtatcgat atccagacgt cctgcggcgt gcagtttcca ggctaccggt tcatccagaa
    20821 aatagagcac cgttgggtga tcagggtcgt ccgtggagca aacatagccc tgaggcttat
    20881 tgagcatgaa gtaacgtgga ccatgttgct gcgccagcgg gttgccatcg taagcgacat
    20941 catgttcagg aagcagtttg aacgctgcat tacgaacaat ttcgccatcg acggtgacac
    21001 gattgccgcg gatttcacgc ccggcaatag cacggctaac gccgagttgc tgtgcgataa
    21061 atttatcaag tcgcatgtgt gtgattttgc ctgtaaaaac ggaggtcggg cattgacccg
    21121 aaaatctgaa ctgttgtctg cccagtatag cggtctaata acatccctca agggaaaaag
    21181 atccatggca tactattagc agaataatct acctacgcga gaccatgatt tttacactcc
    21241 gcccatatca gcaagaagcc gtggatgcca cgctcaacca ttttcgtcgt cataaaaccc
    21301 ctgccgttat cgtgctgccc accggcgcag gtaaaagcct ggtgattgcc gaactggcac
    21361 ggctggcgcg tggtcgtgtt ctggtgctgg cacacgtcaa agaactggtg gcgcaaaacc
    21421 atgcaaagta tcaggcgctg gggctggaag ccgatatttt tgccgctggg ttaaagcgca
    21481 aagagagcca cggtaaagtg gtatttggca gcgtgcagtc ggtcgctcgt aatcttgatg
    21541 cctttcaggg tgaattttcg ctgttgattg tcgatgaatg ccaccgtatt ggtgacgatg
    21601 aagagagcca gtatcagcaa atcctcactc acctgactaa agtgaatccc cacttacgcc
    21661 tgctggggct gactgccacg ccttttcgat tgggcaaagg ctggatctac cagttccatt
    21721 atcacggcat ggtacgcggc gatgagaaag cccttttccg tgactgcatt tatgagctgc
    21781 cgctgcgtta tatgattaaa cacggctatc tgacgccgcc agaacgactg gatatgccag
    21841 tagtgcaata cgatttcagc cgcttgcagg cacagagtaa cgggctgttc agcgaagctg
    21901 atctcaaccg tgagctgaaa aaacagcaac gcattacgcc acatatcatc agccagatca
    21961 tggagttcgc cgaaaagcgc aaaggggtta tgattttcgc cgccaccgtt gaacacgcaa
    22021 aagagattgt gggattactg cctgccgaag atgcagcact gattactggc gacacccccg
    22081 gcgctgagcg cgatgtgtta attgaagatt ttaaagccca gcgttttcgc tatctggtca
    22141 acgtcgcggt actgaccacc ggatttgacg ccccgcacgt cgatcttatc gccattctgc
    22201 gtcctaccga atcagtgagt ctttaccaac aaattgtcgg gcgcggtctg cgtctcgctc
    22261 ctggcaagac tgattgctta attcttgatt atgcgggtaa tcctcacgac ctctacgcgc
    22321 cggaagttgg tacaccgaaa ggcaaaagtg acaacgtccc ggtacaggtt ttctgccctg
    22381 cctgcggttt tgccaacacc ttttggggga aaacgaccgc cgacgggaca ttgattgaac
    22441 actttggtcg tcgctgtcag ggatggtttg aagatgacga cggccatcgc gaacagtgtg
    22501 acttccgttt ccattttaaa aattgcccgc aatgtaacgc ggaaaacgat attgccgccc
    22561 gccgctgccg cgaatgtgac acggtactgg ttgatccgga tgatatgtta aaagcggcgc
    22621 tacgactgaa agacgcgctg gtattacgct gtagcggcat gtctttacag catgggcacg
    22681 acgagaaagg cgaatggttg aaaatcacct attacgatga agacggcgcg gatgtgagtg
    22741 agcgtttccg tctgcaaacg cccgcccagc gaactgcctt cgagcagctt tttatccgcc
    22801 cgcatacgcg cacaccgggc atccctctgc gctggatcac cgccgccgat atcctcgccc
    22861 agcaagcctt actgcgacat ccggattttg tcgtcgcccg catgaaaggt cagtactggc
    22921 aggtgcgtga aaaagtgttc gattacgaag gtcgttttcg tcgggcgcac gaattacgcg
    22981 gttaatggta gttttgattg atgtataagg cgattgagta tagaatctcg cccgcttttg
    23041 catacgcaaa gcagatcact tacctgttgc tgggtcgcct gtagcaggat taatttaaga
    23101 gagaaagaaa tgtttactat caacgcagaa gtacgtaaag agcagggtaa gggtgcgagc
    23161 cgccgcctgc gtgccgctaa caagttcccg gcaatcatct acggtggcaa agcagcaccg
    23221 ctggctatcg agctggatca cgacaaagtc atgaacatgc aagctaaagc tgaattctac
    23281 agcgaagttc tgaccatcgt tgttgacggt aaagaaatca aagttaaagc tcaggacgta
    23341 cagcgtcacc cgtacaaacc gaagctgcag cacatcgact tcgttcgcgc ttaattgctg
    23401 aataagttgt aaaaaacccc gcttcggcgg ggttttttgt atctgcagat tatgcctgat
    23461 tacggtattg ctattttttg caggccagat aaggcgttca cgccgcatct ggcattcaga
    23521 acacaaaacc gattaattgc cgccagacgt ccggcgctgc aattggtcgc gcaaattcgg
    23581 cggcgtgcct ttaatggtca aggtatcggt tgctggatcc cagaagatgc gttcacctaa
    23641 cagcatcgca tcaaagttaa tagtcaagcc accgccgcta ccggcaaact tcgtcaactg
    23701 acgcagtgtg ctacggtctg ccgggaagct ctcttccagt tcgtagcctt tttcagccgc
    23761 aaacttcgtg aagctcactt cgcttacgcc tgccagctct ttcgacagcg attccagttc
    23821 gatctcttcg cctgcctgca gctgctcatt acagtagcta taaacctgct ggcgcacgtt
    23881 ctgtcgctct gctttatcca gttgtgcttc ggcagtgaaa tcatcaaccg cttgcaacag
    23941 cccacggttt tgcgctttcg cgttcaatcc ttcgctggcc ccgaggaaat ccataaagaa
    24001 atccgccact ttgcgcccta ctcgcccttt caggaaagtg agatagcggg tggactctgg
    24061 attggtttcc cattcggtta aatcgatacg cgcaacaata tccgcatgat tgatatcaag
    24121 ataatgggtt gggttgatgt cgagattttc gttaacgcgc atactgctca ggttgctcag
    24181 aactgccacc agcagatact ccaccgccag ataacggtag tggcaaaaca gcacgaatcc
    24241 accgtcagca aaaggatatt tcgctaattc gtcacgcagg cgcccggttg ccgcgcggct
    24301 aaatgccaga aagtcctctt cgccctgacg ctgtaagcgc agcgtctgcg ccagttcact
    24361 ctcttcgcta aacaagccgt aagctttatt tttggcgcta tagacccgat gcagttctgc
    24421 caccatctca acgacggttt ctgtcggttc cagcaatgaa tcgcgcaaca ccagctcaag
    24481 gttctgctca tcacgcttga taagctggtg cagagcaatc tggttgatat ccagactcat
    24541 gataaactct cctttaagac cgggcggtat tcaaccaccg ccggttaagc gacgcaataa
    24601 aagatggcgg tcattttaca gaaggcagcg taacgagaat gatttaacgc ataaaaaagc
    24661 agaaaaaaaa ccgttgctac ggtaatatgt tgccctttca tcaactaact gatttcgatt
    24721 tatgccacaa atttcccgct acagtgatga acaggttgaa caattgctcg cagagctgct
    24781 caacgtactg gaaaaacata aggctccgac cgacctttcc ctgatggtgt taggcaacat
    24841 ggtcactaac cttatcaaca ccagcattgc cccggcccaa cgcctggcaa ttgccaactc
    24901 ttttgcccgc gccttacagt cctctatcaa cgaagacaaa gcgcactaag ggaaacagat
    24961 aacaggttat ggtaactcat cgtcagcgct accgtgaaaa agtctcccag atggtcagtt
    25021 gggggcactg gtttgcactg ttcaatattc tgctttcgct cgtcattggc agccgttacc
    25081 tgtttatcgc cgactggccg acaacgcttg ctggtcgcat ttattcctac gtaagcatta
    25141 tcggccattt cagcttcctg gtgttcgcca cctacttgct gatcctcttc ccgctgacct
    25201 ttatcgttgg ctcccagagg ctgatgaggt ttttgtccgt cattctggca acggcgggaa
    25261 tgacgctatt actgatcgat agcgaagtct ttactcgttt ccatctccat cttaatccca
    25321 tcgtctggca gttggttatc aacccagacg aaaatgagat ggcgcgcgac tggcagctga
    25381 tgttcatcag cgtgccggtt attttattgc ttgaactggt gtttgcgacg tggagctggc
    25441 aaaagctgcg cagcctgacg cgtcgtcgac gcttcgcgcg cccgctggcc gcattcttat
    25501 ttatcgcctt tatcgcctcg catgtggtgt atatctgggc cgatgccaac ttctatcgcc
    25561 cgatcaccat gcagcgcgct aacctgccgc tttcgtaccc gatgacggcg cgacgtttcc
    25621 ttgagaagca tggcctgctt gatgcgcagg agtatcaacg ccgtcttatt gagcaaggta
    25681 atccagacgc cgtttccgta cagtatccgt taagcgaact gcgctatcgc gatatgggca
    25741 ccgggcagaa tgtgctgttg attactgtcg atggcctgaa ctactcacgc ttcgagaagc
    25801 agatgcctgc gctggcaggt tttgctgagc aaaatatttc gttcacgcgc catatgagct
    25861 ccggcaacac tacagacaac ggcatctttg gcctgttcta tggcatctcg ccgagctata
    25921 tggacggcat tctgtcgacc cgtacccctg cggcgttaat tactgcgctt aatcagcaag
    25981 gctatcagct gggattattc tcgtcagatg gctttaccag tccgctgtat cgccaggcat
    26041 tgttgtcaga tttctcgatg ccgagcgtac gcacccaatc cgacgagcag accgccacgc
    26101 agtggatcaa ctggctgggc cgctacgcac aagaagataa ccgctggttc tcgtgggtct
    26161 ctttcaatgg cactaacatt gacgacagca atcagcaggc atttgcacgg aaatatagcc
    26221 gggcggcagg caatgtcgat gaccagatca accgcgtgct caatgcactg cttgattctg
    26281 gcaaactgga caatacggtt gtgattatca ctgccggtcg gggtattccg ctgagcgaag
    26341 acgaagaaac ctttgactgg tcccacggtc atctgcaggt gccattagtg attcactggc
    26401 caggcacgcc ggcgcagcgt attaatgcgc tgactgatca taccgatctg atgacgacgc
    26461 tgatgcaacg cctgctacat gtcagcacac ctgccagcga atattcgcaa ggtcaggatt
    26521 tgttcaaccc tcaacgccgt cattactggg tcactgcagc ggataacgat acgctggcaa
    26581 ttaccacccc gaaaaagacg ctggtgctga acaataacgg taaataccgc acttacaact
    26641 tacgtggtga aagagtaaaa gatgaaaaac cacagttaag tttgttattg caagtgctga
    26701 cagacgagaa gcgttttatc gctaactgat taattataaa tcagttagcg aaatatctta
    26761 cttgcaatcg gtgtggaaaa cggtagtatt agcagccacg agtcggcacg tagcgcagcc
    26821 tggtagcgca ccgtcatggg gtgtcggggg tcggaggttc aaatcctctc gtgccgacca
    26881 aaaatcccaa gaaaaaacca acctttacgg ttggtttttt tatatctgca attaatttga
    26941 taaacagacc gtgacacatc acagcctgtt tattttctgt tatcagaacg tccagaccac
    27001 tcccgcctga gtattccacg gcgattctac ccctgcgcca ttgccatagc tgactgacaa
    27061 atgaccgctt aacgtcgggg taaacgatga ccttatccca gcctgataaa cgccaggggt
    27121 tccagccaca tcattattaa atttgccatc gtcattaact ttcacatggt tagaatcggc
    27181 gtattcctga cgtacggcgg ctttcagcca gggttccagc gtcgtaccat tttgcaggtc
    27241 catgtgatag cttaccgccg ttcccgcttc agcgcgtaat atccgggtat ttcccacatc
    27301 agcgcgcatg ccgtttgata acgtgtagtc ctgaccatct gtggtaaagc cggtaaaggc
    27361 cagatagggt ctaacactcc acaatccgtc aacccaacgg aacccgctct caacatgagc
    27421 acccgcgccg ttactattgt aatcgccaaa cgctgttgcc ccattactca tcttgccatg
    27481 gatggtgttg gcaaaacggt caactttcac caccccatca acataggcac cgttctgatg
    27541 ctcccaaccg gcataagccc ccagggtata gctatcgata ttaccttttc cgccgcgatc
    27601 aaaaccaata tcagaatgag agtaaccaaa gaacaagccg cgaattgtac tgctttcttc
    27661 acgggagaaa cggctatcga taccgagcgt caggcccgtc aatgtttgct caaaaccagc
    27721 tcccgcatca gtggtcacgt tgttgcgggt gttaattgcc gaactccaca tcgccgtatc
    27781 gtaactaacg ctttttacgc taccaagacg ctcacgcacg gtgtccagtt ctgcatcaaa
    27841 taccagcggt tgtgcggccg ccatattcag cacatcagtg gttgaagggg taatttgcgc
    27901 gcgattctct gccaaactcc agctatggtt gccattatcc agcaaggtat attcatacgt
    27961 accgatatca acaacgcctc cggcattgcc caacgtaaat gcagcatcac cgccgcccgt
    28021 tgttaccagt gtaaggctat ctcctgctgc cgggctggca ccggtgtccg tcacgaatat
    28081 tttgaaatca cctgttgcct gaccggtgac gttgagctga tcgctctgat gattagccat
    28141 atcggtacgc atataaaaat tgccgtttcc cgacagggta ttggtggtca acgtaatgaa
    28201 attttccgca cttgccgtca ctgatgaacg agtcactggc tcatccatca taatcacatt
    28261 tgcgtcagac atcgcaaggt tggctaagtt gaggagggtg ttttggttcg tcgtagtgat
    28321 atcggcaatg atggcccata cgctattttc gagcgaaacg tccgctttgc tggtatccac
    28381 ggcaccatgc gttctaaaag aagcgccctc agaaaccact accttaccca ccaacatagc
    28441 gggtgcataa tttatttgcg gcttcatgac ctcaagaatg ccatcattcc ctctgactga
    28501 cacgttaacc attgtgccag cccagacatt agcgcggcca ttgttgataa ccatattttc
    28561 agagatggct ttagaggaat acgtgatact tccattctgg tctgatcgac cgagatcata
    28621 taccgcattt gcctgcactt tcgtaccagt atcctttccc agcgattgca tagtgacatg
    28681 ctcatcaagg atagtatcaa tggcctgcgt gtcctccata acaatgagcc cggaaccatc
    28741 atccagttca taattttttg acacaccatc ttttatacta aattggcctt tactgtttgt
    28801 accactcact tccagcgcat tcgttgagac aattaatttt ccgcctgctt ttttatcaac
    28861 gccagttgca gtcccgccat ccataacctc cagtaagccg ccactatcta cagtagtatt
    28921 ataagcgaag tcattttctt caactcgcaa acttccgccg ttttccaaca gcatattgtt
    28981 agcaatacca ttcttgattt cgaactgacc gagacggttt gttccgaatg cctccatgac
    29041 ccgcgtgctt gctttaatag caccgcctgc tttctgatct accgcaatcg ctaatccccc
    29101 ctctttaacc tccagtaatc ctcccttttc gatagcggca aggacggtaa tcccatcttt
    29161 ttcaacgatt aagcgcccgc ctttaatcat gggatcattc gctgcgccat aaacctgtag
    29221 cacaccatta ttgagggtag tctggtttgc cgcgccaccc atttgaacaa tttgataacc
    29281 gtcatttaat actgagtatt ctgcggtacc attcatttca atgtactgat accctccgtt
//
LOCUS       DAJTNA010000030        29263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTNA010000030 DAJTNA010000000
VERSION     DAJTNA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29263
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.030"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..152
                     /locus_tag="OO161_001141"
     CDS             <1..152
                     /locus_tag="OO161_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HCR5462289.1"
                     /translation="YYGGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLV
                     KSFDW"
     gene            427..3276
                     /gene="rcsC"
                     /locus_tag="OO161_001142"
     CDS             427..3276
                     /gene="rcsC"
                     /locus_tag="OO161_001142"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5462290.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSVAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGGEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRA
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPSEFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVLNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3476..4126)
                     /gene="rcsB"
                     /locus_tag="OO161_001143"
     CDS             complement(3476..4126)
                     /gene="rcsB"
                     /locus_tag="OO161_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5462291.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4143..6815)
                     /gene="rcsD"
                     /locus_tag="OO161_001144"
     CDS             complement(4143..6815)
                     /gene="rcsD"
                     /locus_tag="OO161_001144"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5462292.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDARESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGLGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7554..8684
                     /gene="ompC"
                     /locus_tag="OO161_001145"
     CDS             7554..8684
                     /gene="ompC"
                     /locus_tag="OO161_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5462293.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8796..9851
                     /gene="apbE"
                     /locus_tag="OO161_001146"
     CDS             8796..9851
                     /gene="apbE"
                     /locus_tag="OO161_001146"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5462294.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSVELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9925..10989
                     /gene="ada"
                     /locus_tag="OO161_001147"
     CDS             9925..10989
                     /gene="ada"
                     /locus_tag="OO161_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5462295.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            10989..11639
                     /gene="alkB"
                     /locus_tag="OO161_001148"
     CDS             10989..11639
                     /gene="alkB"
                     /locus_tag="OO161_001148"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5462296.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPNLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDMVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            11715..13358
                     /gene="yojI"
                     /locus_tag="OO161_001149"
     CDS             11715..13358
                     /gene="yojI"
                     /locus_tag="OO161_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5462297.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13576..15222
                     /gene="mqo"
                     /locus_tag="OO161_001150"
     CDS             13576..15222
                     /gene="mqo"
                     /locus_tag="OO161_001150"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR5462298.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15372..15860)
                     /gene="eco"
                     /locus_tag="OO161_001151"
     CDS             complement(15372..15860)
                     /gene="eco"
                     /locus_tag="OO161_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5462299.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESPLKVELLIGQTLEVDCNLHRLGGKLESKTLEGRGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(15995..16159)
                     /locus_tag="OO161_001152"
     CDS             complement(15995..16159)
                     /locus_tag="OO161_001152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462300.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            16268..16762
                     /gene="napF"
                     /locus_tag="OO161_001153"
     CDS             16268..16762
                     /gene="napF"
                     /locus_tag="OO161_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5462301.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16752..17015
                     /gene="napD"
                     /locus_tag="OO161_001154"
     CDS             16752..17015
                     /gene="napD"
                     /locus_tag="OO161_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5462302.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            17012..19498
                     /gene="napA"
                     /locus_tag="OO161_001155"
     CDS             17012..19498
                     /gene="napA"
                     /locus_tag="OO161_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5462303.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            19505..20200
                     /gene="napG"
                     /locus_tag="OO161_001156"
     CDS             19505..20200
                     /gene="napG"
                     /locus_tag="OO161_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5462304.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            20187..21050
                     /gene="napH"
                     /locus_tag="OO161_001157"
     CDS             20187..21050
                     /gene="napH"
                     /locus_tag="OO161_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5462305.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            21047..21496
                     /gene="napB"
                     /locus_tag="OO161_001158"
     CDS             21047..21496
                     /gene="napB"
                     /locus_tag="OO161_001158"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5462306.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            21506..22108
                     /gene="napC"
                     /locus_tag="OO161_001159"
     CDS             21506..22108
                     /gene="napC"
                     /locus_tag="OO161_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5462307.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCRDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            22127..22744
                     /gene="ccmA"
                     /locus_tag="OO161_001160"
     CDS             22127..22744
                     /gene="ccmA"
                     /locus_tag="OO161_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5462308.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            22741..23403
                     /gene="ccmB"
                     /locus_tag="OO161_001161"
     CDS             22741..23403
                     /gene="ccmB"
                     /locus_tag="OO161_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5462309.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            23445..24182
                     /gene="ccmC"
                     /locus_tag="OO161_001162"
     CDS             23445..24182
                     /gene="ccmC"
                     /locus_tag="OO161_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5462310.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            24179..24388
                     /gene="ccmD"
                     /locus_tag="OO161_001163"
     CDS             24179..24388
                     /gene="ccmD"
                     /locus_tag="OO161_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5462311.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            24385..24864
                     /gene="ccmE"
                     /locus_tag="OO161_001164"
     CDS             24385..24864
                     /gene="ccmE"
                     /locus_tag="OO161_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5462312.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24861..26804
                     /gene="ccmF"
                     /locus_tag="OO161_001165"
     CDS             24861..26804
                     /gene="ccmF"
                     /locus_tag="OO161_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5462313.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLLNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVVTIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            26801..27358
                     /gene="dsbE"
                     /locus_tag="OO161_001166"
     CDS             26801..27358
                     /gene="dsbE"
                     /locus_tag="OO161_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5462314.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            27355..28407
                     /gene="ccmH"
                     /locus_tag="OO161_001167"
     CDS             27355..28407
                     /gene="ccmH"
                     /locus_tag="OO161_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5462315.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(28442..29089)
                     /gene="narP"
                     /locus_tag="OO161_001168"
     CDS             complement(28442..29089)
                     /gene="narP"
                     /locus_tag="OO161_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5462316.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         6688 a         7224 c         8105 g         7246 t
ORIGIN      
        1 actactatgg tgggcgcagc gaaacggaat taaccaccgc ccaactgacg ctgatcaccg
       61 atgaaggatc ggtcaatgag aaacaggaaa catttattgt accgatgcgt aatgctggcg
      121 agctgacgct ggtgaaaagt tttgactggt gatccatccc ttcacacaac ctggctctct
      181 cttttttcag ataagactgc tgggcgatct gccgacgcca ggatataacc attaacaagc
      241 gtcggcagat tggtcatttc ttatttttcg tactgagaac tgtccatcta ctccggcttt
      301 cctggttaag gcaatggttt ctaagcgata cgcattattg caataatgac agaacattgc
      361 ggtaacacgc ttttaccgct accttaacca cactccatcg gtcacctgag gcggagcttc
      421 gcccctttga aataccttgc ttcttttcgt acaaccctga aagcctcgcg ctacatgttc
      481 agagcattgg cgttagtgct ctggctgttg attgcttttt catccgtttt ttacatcgtt
      541 aatgcgttac atcagcgaga atcggaaatt cgtcaggaat ttaatctgag ttccgatcag
      601 gctcagcgct ttattcaacg cacctctgat gtgatgaaag agctgaagta catcgccgaa
      661 aatcgcttat cggcagaaaa cggtgtgctt tccccgcgtg gacgagaaac gcaggcggat
      721 gtgcctgcgt ttgaaccgct gtttgccgac tccgattgtt ccgcaatgag taacacctgg
      781 cgaggttctc tggagtcatt ggcgtggttt atgcgctact ggcgcgataa tttttctgtg
      841 gcttacgatc taaaccgggt atttttaatc ggcagcgata acctctgcat ggccaatttc
      901 ggtctgcgtg atatgccagt ggaacgcgat accgcgttga aagctttgca tgaacgcatc
      961 aataaatatc gaaatgcacc acaagatgat agcggcagta acctctactg gatcagcgaa
     1021 ggtccgcgcc ctggcgtcgg gtatttttac gcgttgacgc cagtttatct ggcgaaccgg
     1081 ttgcaggcgc ttttgggggg cgagcagacc atccggatgg agaacttttt cttaccgggt
     1141 acgttgccga tgggggttac cattcttgat gaaaatggtc ataccctgat ttcgcttacc
     1201 ggaccagaaa gtaaaattaa gggcgatcct cgctggatgc aggaacgctc ctggtttggc
     1261 tatacggaag ggttccggga gctggtgctg aagaaaaatc tgccaccctc atcgctaagc
     1321 atcgtgtatt cggtgccggt tgataaggtg ctggaacgca ttcgcatgtt gatccttaac
     1381 gcaattttgc tgaatgtgct tgccggagct gcattgttta ctctcgcacg gatgtacgag
     1441 cgacgtattt tcattccggc ggaaagcgac gccctgcgac tggaagaaca tgagcagttc
     1501 aatcgcaaga ttgtcgcctc cgcgccagtg ggtatctgca ttttgcgtac cgctgatggc
     1561 gtcaatattt taagtaacga actggcgcat acctatctca atatgcttac gcatgaggac
     1621 cgccaacgac tgacgcaaat tatctgtggg cagcaggtca attttgttga tgtcctgacc
     1681 agcaacaata ccaatctgca aatcagcttc gtccattcgc gctatcgtaa tgaaaacgtg
     1741 gccatttgtg tgctggtgga tgtttcttcg cgcgcgaaga tggaagagtc gttgcaggag
     1801 atggcacaag cagcggaaca ggcgagccag tcaaaatcga tgttccttgc caccgtcagt
     1861 catgagctgc gaacgccgct gtatggcatt atcggtaacc tggatctgtt gcaaaccaaa
     1921 gagttaccga aaggcgtcga tcggctggtg acggcaatga acaactcttc cagcctgttg
     1981 ttgaaaatta tcagcgatat tctcgatttc tcgaagattg aatcggaaca gttgaagatc
     2041 gaaccgagtg agttttcacc gcgtgaagtg atgaaccaca tcaccgccaa ctatttaccg
     2101 ctggtggtac gcaagcagtt aggcttgtac tgctttattg aaccggatgt gccagtggcc
     2161 ttaaatggcg acccgatgcg tttacagcag gtcatctcca acctgttgag taacgccata
     2221 aaattcaccg ataccggctg tatagttttg catgtccgcg ctgatggcga ttatctctct
     2281 atccgtgttc gcgataccgg cgtggggatt ccggcgaaag aagtggtgcg cttgtttgat
     2341 cccttcttcc aggtcggaac gggcgtacag cgaaacttcc aggggaccgg tctgggtctg
     2401 gcgatttgtg aaaaactgat cagcatgatg gacggcgata tctcggtaga ttcagaaccg
     2461 ggaatgggca gccagtttac cgtgcgtatt ccgttgtacg gcgctcagta cccgcagaaa
     2521 aaaggcgtgg aagggttgag tggtaaacgc tgctggctgg cggtcctcaa tgcgtcgctc
     2581 tgtcagttcc tggaaactag tttgcagcgc agcggcatcg tcgttacaac atacgaaggg
     2641 caggaaccga ctcccgaaga tgtgttaatt actgacgagg tagtgagtaa aaaatggcag
     2701 ggtagagcgg tagtgacctt ctgtcgtcgc catattggta ttccgctgga gaaagcgcca
     2761 ggggagtggg tacacagtgt ggctgccccg catgagctac cggcattgtt ggcgcgtatt
     2821 tatttgatcg agatggagag cgacgatcct gctaacgctc tgccgtcgac ggacaaagcg
     2881 gtcagcgata atgacgatat gatgattctg gtcgtggatg atcatccgat taaccgacgt
     2941 ctgctggcag atcagttggg atcgttgggc tatcaatgta aaaccgcgaa tgatggtgtc
     3001 gatgcgctta atgtacttag caagaatcat attgatatcg tgcttagcga cgtcaacatg
     3061 ccaaatatgg atggttaccg cttgacgcaa cgcattcgtc agttgggact gacgttgccg
     3121 gtaatcggag taactgctaa tgcgttggct gaagagaagc agcggtgtct ggagtccggt
     3181 atggacagct gcctgtcgaa gccggtaacg ctggatgtga taaaacagac gctgacggta
     3241 tatgccgaga gggtcaggaa atcgcgggaa tcgtaggacg gataaggtgt ttatgtcgca
     3301 tctggcattc agtgcaaatg ccagatgcga cgctgacgcg tcttatctgg cctactttga
     3361 tgtaatctgt tgaaataatg ggaatcgtag gacgcataag gcgtttacgc cgcatccggc
     3421 aatcggtgca aatgccagat aagacactaa cgcgtcttat ctggcctaca ggtgattagt
     3481 ctttatctgc cggacttaac gtcactgaag agagataatt cagcagggcg atatcgttct
     3541 cgacacccag cttcatcatc gcagatttct tctggctact gatggtttta atactgcggt
     3601 tcagcttttt agcgatctcg gtcaccaaga agccttccgc aaacaggcgc agaacttcac
     3661 tctcttttgg cgagagacgc ttgtcaccgt aaccaccagc actgattttt tccaacaggc
     3721 gagaaacgct ttccggggta aatttcttcc ctttctgcag cgcggcgaga gctttcggca
     3781 gatcggtcgg tgcaccttgt ttcagcacga tcccttcgat atccagatcc aataccgcac
     3841 taagaatcgc cgggttgttg ttcatagtca gaacaatgat cgacaggctt gggaaatggc
     3901 gcttgatgta cttgattaag gtaatgccat cgccgtactt atcgccaggc atggagagat
     3961 cggtaatcaa cacatgcgca tccagtttcg gcaggttgtt gatcagtgct gtagagtctt
     4021 caaattcgcc gacaacattc acccactcaa tttgctcaag tgatttgcga ataccgaaca
     4081 agactatcgg atggtcatcg gcaataatta cgttcatatt gttcatgtaa taggctacct
     4141 tgctacagca agctcttgac ataactgtca atgtcgctga tgtatttttc tattcctgga
     4201 acatccttct cacgaatcag atgttccagc gtttcacata actgcttgcc gggtaccaga
     4261 tttagcatgg caaatacgcc tttaagacga tgagccgttt gggctaacgc agcaaagtca
     4321 ctggttgctg cttcagtata cagcctctta acatcatccg gtactgtgtc tacaaagagc
     4381 gcataatagc cgctggcatg gagttgcgca ttttcatctc cgcccagagg cgattctgtc
     4441 acctcttcct gcgccagttg cacttcaatt aattgtaaga ccgcttcctg catagcgttg
     4501 ctcatattga agttgacgca caattgacca agcccaattt cccgtacgcc agactcatca
     4561 tcgcttaaaa gcaagccaga ggcagtaaga ttagacggat tatccgttaa aaagatatca
     4621 taatcttgac taattaatct ttcatcgggt gtgatacagg ttgcacccca attttctaac
     4681 tggcgagtga caatattccg aatttctgcc gaagtaacat ccaccattac gcagacatca
     4741 tccagtaaac gctcttcttc ctcttcaact tccgggtcag cggcgagcat tttgatatgc
     4801 acagagtagc gtgtaccaag cccatcccgc gttttgatgt ttaaatgacc gcccagttta
     4861 cgcgccagtt gatcgctcag ccagaatgcc agcgggtccg ccttgccata gcgatcgttt
     4921 tggggctggt tgataaacgg gaagtgcaaa ttatccattt catgaatact tacgccttct
     4981 cccgtgtcca gaatgcggaa tgtcaggcgg tcttcagagg actcatcctg atcaacctca
     5041 agggtgattt ttcccaattg cgttgaggtc acggcatatt gcatcagtag cagcaaaata
     5101 cgtcgtaagg catcgcgatc gccgcggcgc atatcgtgtg ctttcagatg attgttaatc
     5161 agcagttgca gacctttacg cttgatggca ggcaacactg aaggcacaac ttcatcaatt
     5221 aaatcctgca cggagaacag caccgtctca cttttccagc tatcgtccgc aagcatgttc
     5281 gctaactgta tttcatcgac cagccgcacc agcacatctg cctgattcgc cagttgtttg
     5341 ctttccgggg cgttgagttt agccgcgctc tccgccaggg actgtgcggg ttctttcagc
     5401 gcatcgccaa tgtttttcat aaaggtcatc cgcccctgct ggtttttctc atacagacgc
     5461 tgcgcctgct tgagtttctt gtttaccagc acttcgcgat cctgatcgcg aataatgaaa
     5521 atttgtgtgc gcggcgcgac ctggctgcgg aacatgcgga tctcatacag ctcgttattg
     5581 atcgtcgcct gaataatccc ctgatgctgt tccgccatgg tggtgatgtt ttgcagattc
     5641 aaatgcggca gcaaatgatc ggcaattttg ttacttatga cagtgcggtt cgattcctga
     5701 tcgtgaacca gcaggccgag cggcagcagt gagactatct cttcattaat tgcccgtaaa
     5761 atgcgcaatt cgttattgac cgccgtgctg ggtagacttt ctgtactgcg gctggagaaa
     5821 tggcggaatg tggtatagcc aaataacgcc agcgccagca aaccgatgtt cagcagcagt
     5881 ggcagcagaa tgttttgcaa cgtatccagc aataaggtgc cataaggaac ctgccagacc
     5941 aggcgcatat cggtagagtt gagtgccgag gagatttcaa tcttcgtact gttaaagtgg
     6001 atactgacgc tatccgtccc ttctttctca ttatcattgt ctcccgttgc cgtcgcgtct
     6061 ggctcaaggc ggaaactgtc cagcggcata cccggtggga tcaaatcatt aatcggtaga
     6121 tcaaaagcta cgaccgttgc cagatgtccc ggctggttga acgtggtacg caaggtaaag
     6181 taatgaccgt tctgccaggc caggcggcgc atgttagaaa aactttcgcg tgcatcgagg
     6241 gcgttggcct gttgcaacat ctctgcacga cgtgaatcaa caatgtcgct gacggtcgat
     6301 tctttaaatc cggaggtgag atctttgagg ggcagggttg agatcagcac cagactgtta
     6361 tcctgaccat tcaggtaata catcgaccac ggtacatttt ctgcgcccca caatgtgtcc
     6421 agataggtgg acatccgctg agtcatctca agcgttgagt tgtcgtgaga gccaaagatt
     6481 aacgcttccg ttttgcggcg cggtttctcc agatagtaga catcctgttt caggcgcgtc
     6541 tcttgtaaac cttcgccgga ggagggggag gtcgtcgcgg caatgttgtc gtagatctgc
     6601 caggtcacgt aacgccaggt attgacgcgc ttttggatag cgtgggtaat gtcgacaatc
     6661 tggtaacttt tatctttcag ccatgcgtta acggcgcttt gtaccattac acccatcgtc
     6721 accagtaaca caatgatcaa cagtaaaaag aagcgggtaa tgctccccgg taggagtgaa
     6781 aagcgggtcg tggccgttgt ctctttctga cgcattcgtg tttatgacct gttaaaactt
     6841 cgcgaagtga tagggcagta taaagggtac agtgtgaaat tccagactct tacctgattg
     6901 atgattctct attctcccgt gatatttatc atcctgcatt gggggaaata gatgtacaag
     6961 cgccattttg ctgtacaaat aacctacaaa aagcccaacg aaattaattg caaataaacg
     7021 atgattgctg gaaattatgc tgatgttaat tatttgtgaa atagttaaca agcgttatag
     7081 tttttctgtg gtagcacaga ataatgaaaa gtgtgtaaag aagggtaaaa aaaaccggat
     7141 gcgaggcatc cggttgaaat aggggtaaac agacattcag aaatgaatga cggtaataaa
     7201 taaagttaat gatgatagcg ggagttattc tagttgcgag tgaaggtttt gttttgacat
     7261 tcagtgctgt caaatactta agaataagtt attgatttta accttgaatt attattgctt
     7321 gatgttaggt gcttatttcg ccattccaca ataatcttaa aaagttccct tgtatttaca
     7381 ttttgaaaca tctatagcga taaatgaaac atcttaaaag ttttagtatc atattcgtgt
     7441 tggattattc tgcatttttg gggagaatgg acttgccgac tgattaatga gggttaatca
     7501 gtatgcagtg gcataaaaaa gcaaataaag gcatataaca gagggttaat aacatgaaag
     7561 ttaaagtact gtccctcctg gtcccagctc tgctggtagc aggcgcagca aacgctgctg
     7621 aagtttacaa caaagacggc aacaaattag atctgtacgg taaagtagac ggcctgcact
     7681 atttctctga caacaagtca gaagacggcg accagaccta tgtacgtctt ggtttcaaag
     7741 gcgaaactca ggttactgac cagctgaccg gttacggcca gtgggaatat cagatccagg
     7801 gcaatacctc tgaagacaac aaagaaaact cctggacccg tgtggcattc gcaggtctga
     7861 aattccagga tgtaggttct ttcgactacg gtcgtaacta cggcgttgtt tacgacgtaa
     7921 cttcctggac cgacgtactg ccagaattcg gtggcgacac ctacggttct gacaacttca
     7981 tgcagcagcg tggtaacggc ttcgcgacct accgtaacac cgacttcttc ggtctggttg
     8041 acggtctgaa ctttgctgtt cagtaccagg gcaaaaacgg tagcgtaagc ggcgaaggca
     8101 tgaccaacaa cggtcgtggc gctctgcgtc agaacggcga cggtgtcggc ggttctatca
     8161 cttatgatta cgaaggcttc ggtatcggtg ctgcagtttc cagctccaaa cgtactgatg
     8221 atcaaaatgg tagctacatc agcaatggtg tagttcgtaa ctacatcggt actggcgacc
     8281 gtgctgaaac ctacactggt ggtctgaaat acgacgctaa caacatctac ctggctgctc
     8341 agtacaccca gacctacaac gcaactcgcg taggttccct gggttgggcg aacaaagcac
     8401 agaacttcga agctgttgct cagtaccagt tcgacttcgg tctgcgtccg tctgtagcat
     8461 acctgcagtc taaaggtaaa aacttgggtg tcatcaatgg tcgtaactac gacgacgaag
     8521 atatcctgaa atatgttgat gttggcgcga cctactactt caacaaaaac atgtccacct
     8581 atgttgacta caaaatcaac ctgctggacg acaaccagtt cactcgtgac gctggcatca
     8641 acactgataa catcgtagct ctgggtctgg tttaccagtt ctaatctcga ttgatatcga
     8701 acaaagggcc tgcgggccct ttgttcattg ttttcagcgt acaaactcag ttttttggtg
     8761 tactcttgcg accgttcgca tgaggataat cacgtatgga aataagcttt acccgcgtgg
     8821 cactgctggc tgccgcgctc ttctttgttg gttgcgatca aaaaccacaa cccgccaaaa
     8881 cccacgctac tgaagttacc gttcttgaag gcaaaaccat gggtaccttc tggcgtgcca
     8941 gcatcccggg cattgatgcc aaacgcagtg tcgaacttaa agaaaagatt cagacccagc
     9001 tggacgccga cgatcagctg ctttcgacct ataaaaaaga ttccgcgctg atgcgcttta
     9061 acgactcgca aagtttgtcg ccgtggccag taagtgaagc gatggccgaa atcgtcacta
     9121 cctcgctgcg cattggcgcg aagaccgatg gtgcgatgga tattaccgtc gggccgctgg
     9181 taaatctgtg gggctttggc ccggaacaac agccggttca aattccgagc caggaacaga
     9241 tcgacgagat gaaagccaaa actggtttac agcaccttac ggtcattaat cagtcgcatc
     9301 agcaatatct gcaaaaagac ctgccggatt tatatgtcga tctctctacc gtcggcgaag
     9361 gttatgcggc ggatcacttg gcacgcttga tggagcagga agggatttcc cgctatctgg
     9421 tgtcggtggg cggcgcgctg aacagccgtg gtatgaacgg tgaaggcctg ccgtggcggg
     9481 tagcgattca aaaaccaacc gataaagaaa acgcagttca ggctgtggtg gatatcaacg
     9541 gtcatgggat tagcacctct ggcagctacc gtaactatta cgaactggac ggcaaacgtc
     9601 tttcccatgt tatcgatccg caaaccgggc gtcccatcga acataatctg gtatccgtga
     9661 cggtgattgc tccgacggcg ctggaagccg atgcctggga tactggcttg atggtactcg
     9721 ggccggagaa agccaaagag gttgttcgcc gggaagggct ggcggtttat atgatcacca
     9781 aagaaggcga tagctttaaa acctggatgt caccacagtt taaaagcttc cttgtcagcg
     9841 aaaaaaatta aagcgcaaga ttgttggttt ttgcgtgatg gtgaccgggc agactaaagg
     9901 ctatccttaa ccagggagct gattatgaaa aacgccacat gcttaactga cgatcaacgc
     9961 tggcaatctg tcttagcccg cgacccgaat gccgacggcg aattcgtttt cgccgtgcgc
    10021 accacaggca tcttttgccg tccgtcttgc cgcgccagac atgccttgcg ggaaaatgtc
    10081 tccttctacg caaatgccag cgaggcactc gccgccggat ttcgcccctg caaacgctgc
    10141 caacctgata aagccaatgc ccagcaacat cggttggata aaatcaccca cgcgtgtcga
    10201 ctgctggaac aggaaacgcc tgtaacgttg gaagccttag ccgaccaggt ggcgatgagt
    10261 ccatttcatc tacatcggtt gtttaaagcg actaccggaa tgacgcctaa agcctggcaa
    10321 caggcctggc gcgctcgccg tttgcgcgaa tcgctggcga aaggggagag cgtgacgacg
    10381 tctattctta acgccggatt ccccgacagc agcagttact accgcaaagc cgatgaaacg
    10441 ctgggcatga cggctaaaca attccgtcac ggtggcgaaa atctggcggt gcgttacgcg
    10501 ctggctgatt gtgcgctggg tcgttgcctg gtggcagaaa gcgagcgggg gatttgcgcg
    10561 atattgctgg gcgatgatga cgcgacatta atcagcgagt tgcagcagat gtttcccgct
    10621 gccgacaacg cgcctgccga tctgacgttt cagcaacatg tgcgtgaagt gatcgccagc
    10681 ctcaatcaac gcgatacgcc gctgacgtta ccgctggata ttcgcggcac tgcttttcag
    10741 caacaagtct ggcaggcact gcgcacgata ccttgcggtg aaaccgtcag ttatcagcaa
    10801 ctggctaacg ccatcggcaa accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc
    10861 aacaagctgg ctatcgtaat accctgtcat cgagtggtcc gtggtgatgg cacactttcc
    10921 ggttaccgct ggggcgtgtc gcgtaaagcg caactgctgc gccgcgaagc tgaaaatgag
    10981 gagaggtaat gttggatctg tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg
    11041 gtgcggtaat tttacggcgt tttgctttta acgctgcgga gcaactgatc cgcgatatta
    11101 atgacgttgc cagccagtcg ccgtttcgcc agatggtcac ccccggggga tataccatgt
    11161 cggtggcgat gaccaactgt gggcatctgg gctggacgac ccatcggcaa ggttatctct
    11221 attcgcccat tgatccgcaa acaaataaac cgtggcccgc catgccacaa agctttcatg
    11281 atttatgtca acgtgcggct acggcggcgg gctatccaga tttccagcca gatgcttgtc
    11341 ttatcaaccg ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccga
    11401 atctgcgcgc gccaattgtt tctgtttctc tgggcttacc cgcgattttt caatttggtg
    11461 gcctgaaacg taatgatccg ctcaaacgtt tgttgttgga acatggcgat atggtggtat
    11521 ggggcggtga atcgcggctg ttttatcacg gtattcaacc gttgaaagcg gggtttcatc
    11581 cactcaccac cgactgccgc tacaacctga cattccgtca ggcaggtaaa aaagaataaa
    11641 aataagaatt attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg
    11701 tccgggtttt ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc tggccattta
    11761 tcagtgtgat ggcgctaagc ctcgccagtg cggcattagg cattggctta attgctttta
    11821 tcaatcagcg ccttatcgaa atggcggata ccagtctgct ggtattgccg gagtttctgg
    11881 gattattgct gctgctgatg gcagtcactc tcggatcgca actggcgctc accactttgg
    11941 ggcatcactt cgtttaccga ctgcgtagtg aatttatcaa gcggattctg gatacccacg
    12001 tcgagcgcat tgaacaactc ggtagcgcct cgttgctggc gggattaacc agcgatgtgc
    12061 gcaatatcac cattgctttt gtgcgtcttc cggaactggt gcaggggatc attctcacta
    12121 tcggttccgc ggcgtatctg tggatgctgt cgggcaaaat gttgctggta acggctatct
    12181 ggatggcgat caccatctgg ggtggttttg tactggtggc gcgggtgtac aaacatatgg
    12241 cgaccctacg tgaaaccgaa gacaagctgt acacggattt tcaaactgta cttgaagggc
    12301 gcaaagagct gactctgaac cgggaacgcg ccgagtatgt gtttaacaac ctctacatcc
    12361 ccgatgcgca agagtatcgc catcatatta ttcgcgcaga caccttccat cttagtgccg
    12421 tgaactggtc aaacatcatg atgctgggcg ctatcggcct ggtgttctgg atggcgaaca
    12481 gtctcggttg ggctgatacc aacgttgctg cgacctattc gttgacgctt ttattcctgc
    12541 gtacgccgct gctttcggcg gttggcgcat tgccgacgct gctgacggcg caggtggcgt
    12601 ttaacaagct gaacaaattc gcgctcgcgc ctttcaaagc agagtttccg cgcccgcagg
    12661 cgtttcccaa ctggcaaacg ctggagctgc gtaatgtgac gtttagctat caggataacg
    12721 cgttttccgt tggtccgatt aatctcacca tcaaacgtgg cgagctactg tttctgattg
    12781 gtggtaacgg tagtggaaaa tcgacgctgg cgatgttgtt gacgggcttg tatcagccac
    12841 aaagcggcga aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc
    12901 gcaaactgtt ttcggcagtg tttaccgatg tctggctgtt tgatcaactg ctggggccgg
    12961 agggtaaacc cgctaacccg caactggttg agaagtggct ggcgcagctg aaaatggctc
    13021 ataagcttga gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa
    13081 aacgcgtggc gctgttgctg gcgctggcag aagaacgcga tattatcctg ctggatgaat
    13141 gggcggcgga tcaggatcca cacttccgtc gtgagtttta tcaggtgttg ctgccgctga
    13201 tgcaggagat gggtaaaact attttcgcta tcagtcatga tgatcattac tttatccacg
    13261 ccgaccgcct gctggaaatg cgcaatgggc aacttagcga gctgacgggc gaagagcgcg
    13321 atgccgcttc gcgtgatgcc gttgcccgga cggcataata ttacgacgct gacctggcgg
    13381 actgctgccg tcaggtcaat atgctaaaaa tcccttcatt tctaataacc ctataattaa
    13441 tcaacgaaat tataatgttt ctaaaattag aatataattt ataaacatta tttaaatgtt
    13501 gttacttaag tgttaccttt gatgccgcgc aaatctcacc tgcaataaag cgactaaaag
    13561 taaggcatta acaagatgaa aaaagtgact gccatgctct tctcgatggc cgtggggctt
    13621 aatgccgttt cgatggcggc aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg
    13681 ttgattggcg gcggcattat gagcgccacg ttggggacct atttacgcga gctggagcct
    13741 gaatggtcga tgaccatgct ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc
    13801 tggaataacg ccggaaccgg gcattctgca ctgatggaac tgaactacac cccgcaaaac
    13861 gccgatggca gtatcagtat tgaaaaagca gtcgccatta acgaagcatt tcagatttcc
    13921 cgccagttct gggcgcacca ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc
    13981 aataccgttc cgcatatgag ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc
    14041 cgttacgccg cgttgcaaca aagctcgctg tttcgcggta tgcgttactc tgaagatcac
    14101 gcgcagatca aagagtgggc accgttagtg atggaagggc gcgatccgca acagaaagtg
    14161 gcagccacgc gtacggaaat tggtaccgat gtgaactacg gcgagatcac ccgccagtta
    14221 attgcttcct tgcagaagaa atctaacttc tcgctgcaac tcagcagcga agtccgcgcc
    14281 ctaaagcgta atgacgataa cacctggacc gttaccgttg ccgatctgaa aaatggcact
    14341 gcacagaaca ttcgtgccaa atttgtcttt atcggcgcgg gcggtgcggc gctgaagctg
    14401 ttacaggaat cggggattcc ggaagcgaaa gactacgccg gtttcccggt gggcggacag
    14461 tttcttgttt cggaaaaccc ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa
    14521 gcatccgttg gcgcaccacc gatgtcggtt ccgcatatcg atacccgcgt tctggacggt
    14581 aaacgcgtag tgctgtttgg gccatttgcc accttctcaa ccaaattcct caaaaacggt
    14641 tcattgtggg atctgatgag ttccaccacc acctctaacg tgatgccgat gatgcacgtc
    14701 gggctggata atttcgatct ggtgaaatat ctggtgagtc aggtgatgtt gagtgaagag
    14761 gatcgttttg aagcgttgaa agagtactat ccgcaggcga aaaaagagga ctggcgtttg
    14821 tggcaagcgg ggcagcgtgt gcagattatc aagcgtgatg ccgataaagg tggtgtactg
    14881 cgtctgggta ctgaagtggt cagtgaccag caagggacca ttgccgcgct cctgggagca
    14941 tcgccagggg cgtcaaccgc cgcgccgatt atgttggatc tgctggaaaa agtatttggc
    15001 gatcgcgttt ccagcccgca atggcaggct acgttgaaag cgatcgttcc gtcttatgga
    15061 cgcaagctga acggtgatgt agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg
    15121 ctggggctga aatacgacaa gccgcaggca gcagatagta cgccaaaacc gcagttgaaa
    15181 ccgcaacccg ttcaaaaaga agtggcggat attgcgttgt aatgatgtgc cacatccggc
    15241 atggtatgcc ggatgtggcg tatactgata agacgcgcca gcgtcgcatc aggcaaccgg
    15301 ctcgggcgtt agatgttgga tgcggcgtaa ccgccttatc caaccaacgg ggtgccgtac
    15361 ttcacaacag tttagcgaac ttctgcgttg tcaattttct cttccgcctt ccagacgcgg
    15421 tatttcacat ctacattgtc tggcgtatac accacgatcg gcagcttgct gttgtaacgc
    15481 agcattccag catcgcccag atacgcggtg acaaatttct tctctttctt gccatccggg
    15541 caggccatca tcgtggaaac cggcgagcta actttatcaa agacataata atcatagccc
    15601 cggccttcca gcgttttgct ttccagcttc ccgccgagac gatgcaaatt gcaatcgact
    15661 tccagcgtct gaccgattaa cagttctact ttcaggggag attcatcttc ttgcggggtt
    15721 aactgaatca cctgacgctt catccctttt tcagcttgtg gataaggcgc gattttttcc
    15781 agtggctgga cgctttctgc cgcccaggcg gaagtggtag cgaaagcggc aaacaatact
    15841 gcaggtagaa tggtcttcat ttttttgctt tccttcttca tttataggtt ttcaccgatg
    15901 atgtcgctga aggttaacat attcataaga tttattaatt tgctttatga ataacttaaa
    15961 gaatattcag acaattttac ctgctctttt agtgttaaat tctgatgaga gaacaaacca
    16021 gcagaaatat ctctggtgaa ttaattgcta tataaatatg tttataacca tttgaaatgt
    16081 gagcaaaagc ccgtttttgc cacgcttccc gccactcttt tgatcctgct acaggtttta
    16141 ccccgatcgg ggtatgcatc tttgacacat cctttaatat cttagcggtt ataaaaatgg
    16201 cttattaatt atgcggttta tttggtcgct ctcaattttc agagcgcgtt aatgatggaa
    16261 ggtcaatgtg aagattgatg catcccgtcg gggcatactc actggtcgct ggcgcaaagc
    16321 cagtaacggt atccgtccgc cctggtccgg tgatgaatct cattttctga cccattgcac
    16381 ccgttgcgac gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg
    16441 ctatccgagc gttaatttca aaaataacga gtgcagcttc tgttacgctt gcgcgcaggc
    16501 ttgccccgaa tcgctatttt ctccgcgcca caccagggcc tgggatttgc agttcaccat
    16561 tggggacgcg tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg atagctgtga
    16621 gccaatggca attatctttc gcccgacgct ctccgggatc taccagccgc aacttaatag
    16681 ccaactttgc aacggatgtg gagcatgtgc agccagctgt ccggtatcag ccataactgc
    16741 ggagtatctc catgcacact aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg
    16801 aacgaatttc agacatcagc acccaactga acgcctttcc cggctgtgaa gttgctgtca
    16861 gcgacgcgcc gagcggtcag ttgattgtgg tggtggaagc agaagacagc gaaacgctga
    16921 tccaaaccat tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc
    16981 accagcagga agagcaaggt gaggaaacac catgaaactc agtcgtcgta gctttatgaa
    17041 agctaacgcc gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg
    17101 cgccgttgtt ggtcagcagg aagccatcaa atgggataaa gcgccgtgcc gtttctgcgg
    17161 tactggttgc ggcgttctgg tcggaacgca gcagggacgt gtggtggcct gtcagggcga
    17221 cccagacgca ccggttaacc gtggcctgaa ctgcattaag ggctatttcc tgcccaagat
    17281 catgtacggt aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga
    17341 caaagaaggc gaatttaccc taatcacctg ggatcaggcc ttcgatgtga tggaagagaa
    17401 attcaaaacc gccctgaaag aaaaagggcc ggaatcgatc ggtatgttcg gttctggtca
    17461 gtggactatc tgggaaggtt atgccgcgtc caagctgttt aaagcgggct tccgttcgaa
    17521 caacatcgac ccgaacgcgc gtcactgtat ggcgtcggca gtagttggct ttatgcgtac
    17581 ctttggtatg gatgagccga tgggctgcta tgacgacatc gagcaggctg acgcgtttgt
    17641 gctgtggggc gcaaacatgg cggagatgca cccgatcctc tggtcgcgca tcactaaccg
    17701 tcgtctctct aatcagaacg tcaccgtggc ggtgctttct acctaccagc atcgtagctt
    17761 cgagctggcg gataacggca tcatctttac gccacagtct gacctggtga tcctgaacta
    17821 catcgccaac tatatcattc aaaacaatgc gataaatcag gacttcttca gcaaacacgt
    17881 taacctgcgc aaaggggcga cggacattgg ctacggttta cgtccgaccc atccgctgga
    17941 aaaagcagcg aagaatccag gttctgacgc ctccgaaccg atgagctttg aagattacaa
    18001 agccttcgtt gccgagtata cgctggaaaa aactgccgaa atgaccggcg tgccgaaaga
    18061 ccagttagaa caactggcgc agctgtatgc cgatccgaac aagaaagtca tctcctactg
    18121 gacgatgggc ttcaaccagc atactcgtgg cgtgtgggct aacaacctgg tctacaacct
    18181 gcacctgctg accggcaaaa tttcccagcc gggttgcggt ccgttctccc tgaccgggca
    18241 accttctgcg tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga
    18301 catggtggtg accaacgaga aacaccgtga tatctgcgag aagaagtgga atatcccgag
    18361 cggcaccatt ccggcgaaaa tcggtctgca cgcggtggca caagaccgtg cgctgaaaga
    18421 cggcaaactg aatgtttact ggaccatgtg taccaacaac atgcaggccg ggccgaacat
    18481 caatgaagag cgtatgccgg gctggcgcga tccgcgcaac ttcatcatcg tctccgatcc
    18541 gtatccgaca gtcagtgcgc tggccgccga cttgatcctg ccgaccgcaa tgtgggtaga
    18601 gaaagagggc gcttacggta acgccgaacg ccgtactcag ttctggcgtc aacaggtaca
    18661 ggcaccgggc gaagcgaaat cggatctctg gcagttagtc cagttctccc gccgcttcaa
    18721 aactgaagaa gtatggccgg aagatctgct ggcgaagaaa ccggaactgc gtggcaaaac
    18781 gctgtacgaa gtgctgtatg caacaccgga agtgagcaaa ttcccggtat ccgaactggc
    18841 ggaagatcag ctgaacgatg aatcccgcga gctgggcttc tatctgcaaa aagggctgtt
    18901 cgaagagtac gcatggtttg gtcgcggtca cggtcacgat ctggcaccgt tcgatgacta
    18961 ccacaaagcg cgcggtctgc gctggccggt ggtgaacggt aaagaaacgc agtggcgtta
    19021 cagcgaaggt aacgacccgt acgtgaaagc gggcgaaggc tataagttct acggcaaacc
    19081 ggatggcaaa gcggtgatct tcgcactgcc attcgaaccg gcggcggaag caccggatga
    19141 agagtacgac ctgtggctct ctaccggacg cgttctggag cactggcaca ccggcagtat
    19201 gactcgccgt gtaccagaac tgcaccgcgc cttcccggaa gcggtcctgt ttattcaccc
    19261 gctggatgcg aaagcgcgcg atctgcgtcg tggcgacaaa gtgaaagtgg tttctcgccg
    19321 tggcgaagtg atctcgattg tcgaaacgcg cggtcgtaac cgtccgccgc agggcctggt
    19381 gtacatgccg ttcttcgacg ccgcacagct ggttaacaaa ctaacgctgg acgcgaccga
    19441 tccgctctcg aaagagacgg acttcaagaa gtgcgcagtc aaactggaga aggtgtaagc
    19501 cattatgtcc cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc gcgatgttgt
    19561 tcgcacagca ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc
    19621 acgcgcatct ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg cctttgccag
    19681 tgcctgtgtg cgttgcggtc agtgtgttca ggcttgccct tacgacacct taaaactggc
    19741 gacgctggcc tctggtctgt cggcgggcac gccatatttt gtcgcacggg atattccttg
    19801 cgaaatgtgt gaggacattc cgtgcgccaa agtgtgcccg agcggtgcgc tggatcgtga
    19861 gatcgaatcg atcgacgacg cgcggatggg gctggcggta ctggtggacc aggaaaactg
    19921 tctcaacttt caggggctgc gctgcgatgt ttgttatcgc gaatgcccga aaattgatga
    19981 ggccatcacc ctggagctgg agcgcaacac gcgtaccggt aagcacgccc gctttctgcc
    20041 gacggttcac agagacgcct gtaccggttg cggtaagtgc gaaaaagtgt gcgtgctgga
    20101 acaaccggca atcaaggtgt taccgctgtc actggctaaa ggggagttag gtcaccatta
    20161 ccgcttcggc tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg
    20221 cgctggagaa aaaaggctgg tggcgcagtc accgttggct ggtgttacgt cgcctttgtc
    20281 agttcttcgt gctggggatg tttttgagcg gtccgtggtt tggtgtgtgg atcttacacg
    20341 gtaactacag cagtagcctg ttattcgaca ccgttccgct gaccgatccg ctgatgacgc
    20401 tgcaaagtct tgccagcggt catctgcctg ccacggtggc gttgaccggt gcggtgatta
    20461 tcaccgtgct ctacgccctg gcaggaaagc gattattttg cagctgggtc tgcccgctga
    20521 atccgattac cgacttagcg aactggttac gcaggcgctt tgacctcaat cagtctgcga
    20581 cgatccctcg ccacattcgc tacgtgctgt tggtggtcat tctgctggga tcggccttaa
    20641 ctggcacgct catctgggaa tggatcaacc ccgtatctct gatggggcgc agcctggtaa
    20701 tgggcttcgg cagcggcgcg ctgcttattc tcgcgctgtt tttatttgat ctactggtcg
    20761 ttgaacacgg ctggtgcggg cacatttgtc cggttggcgc gctgtatggc gtgctgggta
    20821 gcaaaggtgt gattaccgtt gcggccaccg atcggcagaa atgtaaccgc tgtatggatt
    20881 gttttcatgt ttgcccggaa ccgcgtgtgc tacgtgcccc ggtgctggat gaacaaagcc
    20941 cggtgcaggt caccagccgc gattgcatga cgtgcggtcg ctgcgtggat gtctgttctg
    21001 aggatgtatt tacaataact acacgatgga gttcgggagc gaaatcatga aaagccatga
    21061 cctgaagaaa gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa gcggcgcggt
    21121 ttgggcggct aatggcgtcg attttagcca gtcgccggaa gtatccggga cacaggaagg
    21181 ggccattcgg atgccaaaag agcaggaacg gatgccgctg aactatgtga atcagccgcc
    21241 aatgatcccg catagcgttg aaggttatca ggtaacgacc aataccaacc gctgcttgca
    21301 gtgccacggt gtcgaaagtt atcgcaccac tggcgcgccg cgcattagtc ctactcactt
    21361 tatggacagc gacggcaaag tgggcgcgga agtggctccg cgtcgttatt tctgtctgca
    21421 atgtcacgta ccgcaggccg ataccgcgcc aatcgtgggg aataccttta ccccatcaaa
    21481 aggttacggg aaataagagg ttattatggg aaattctgac cgtaagcctg gtctgattaa
    21541 gcgcctgtgg aaatggtggc gtacccccag ccgtctggcg ctggggacgc tgctgttgat
    21601 cggttttgtt ggcggcatcg tcttctgggg tggctttaac accgggatgg aaaaagccaa
    21661 taccgaagag ttctgcatta gctgccacga aatgcgcaac acggtgtatc aggaatacat
    21721 ggattccgtg cactacaaca accgtagcgg cgtccgtgcg acctgtcggg attgtcacgt
    21781 tccgcacgag tttgtgccga agatgatacg caagctcaaa gcaagtaaag agctgtatgg
    21841 taaaattttt ggcgttattg acactccgca gaaatttgaa gctcatcgtc tgacgatggc
    21901 acagaatgag tggcggcgca tgaaggacaa taactcgcag gagtgccgta actgtcacaa
    21961 cttcgagtat atggatacaa ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc
    22021 ggtgaaagat gggcaaacct gtattgattg ccataaaggg atagcgcaca agctgcccga
    22081 tatgcgtgaa gtcgagccag gtttttaaca gggttattgc gtgggtatgc ttgaagccag
    22141 agagttactt tgtgagcggg atgaacgaac cttatttagt ggcttgtcat ttacgctgaa
    22201 cgcaggagag tgggtacaaa tcaccggtag caacggcgcg gggaagacaa cgcttctccg
    22261 tttgctgacg gggttgtctc gccctgacgc aggcgaggtt ctctggcaag gacagccctt
    22321 gcatcaggta cgcgacagct accatcaaaa cctgttatgg ataggccatc agccggggat
    22381 caaaacccgg ctgacggcgt tagaaaatct gcacttttat catcgcgatg gcgataccgc
    22441 acaatgtctg gaagccctgg cgcaggccgg gcttgccgga ttcgaagata ttcctgtaaa
    22501 tcagctctcg gccgggcaac aacgccgcgt cgctttagcg cgtctgtggc tgacccgtgc
    22561 cacgttatgg atcctcgacg agccttttac cgcgattgac gttaacggcg tcgatcgtct
    22621 gacccagcgt atggcgcagc atacggagca gggggggatt gtgattctga ctacccacca
    22681 gccgctcaac gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa cgagggccgc
    22741 atgatgttct ggcgcatttt ccgtcttgag ctgcgtgtag cgtttcgcca tagcgccgaa
    22801 atcgccaacc cgctgtggtt cttcctgatt gtaattaccc tttttccgct cagtatcggt
    22861 ccggagccgc aactgctggc gcgtattgca ccgggcatta tctgggttgc tgcgctgctt
    22921 tcatccttgc tggcgctgga acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa
    22981 caattgatgt tgttgccgtt acccttgccc gccgttgtgc tggcgaaggt gatggcgcac
    23041 tggatggtaa ccggtctgcc gttactcatc ctttcgccac tggtagcaat gctactggga
    23101 atggatgttt atggctggca agtgatggcg ctgacgctgc tgctgggaac gcctacgctt
    23161 ggctttctcg gtgcaccggg tgtggcgttg acagtgggac ttaagcgcgg tggtgtgctg
    23221 ctcagcatac tggtgttacc gctgactatc ccattactca tctttgccac cgccgcgatg
    23281 gacgcggctt ccatgcattt gcccgttgac gggtatctgg caattttagg cgcgttgctg
    23341 gcaggcaccg cgacattaag tccttttgcg acggcggcag cgttacgaat cagcattcaa
    23401 taacgctgga ttactgattt ttgttcgagt ctggtatcga aactatgtgg aaaacactgc
    23461 atcaactggc gatcccacca cggctgtatc aaatctgtgg ctggtttata ccgtggctgg
    23521 caattgccag tgtggtcgtg cttaccgtcg gctggatctg gggattcggc tttgctccgg
    23581 ctgattatca gcagggaaat agctaccgca ttatctacct gcatgtgcct gcggcgatct
    23641 ggtcgatggg tatttatgca tcaatggcgg tggcggcgtt tattggcctt gtctggcaga
    23701 tgaaaatggc caacctggcg gtggcggcga tggcccccat tggtgccgtg tttaccttta
    23761 ttgccctggt taccggctct gcatggggaa aaccgatgtg gggcacctgg tgggtatggg
    23821 atgcacgtct gacttctgaa ctggtgctgc tgtttttgta tgtgggtgtg attgccctgt
    23881 ggcacgcctt cgacgaccgc cgtctggcgg gccgtgcggc aggtatcctg gtgctgattg
    23941 gcgtggtgaa tctgccgatt attcattact ccgtggagtg gtggaacacc ctgcatcagg
    24001 gatcaacgcg gatgcagcaa agtatcgatc cggcgatgcg ttcgccgctg cgctggtcga
    24061 tttttggctt cctgctcctg tctgccacgc tgacgctgat gcggatgcgt aatttgattt
    24121 tgctgatgga aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat
    24181 gacccctgca tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt
    24241 ctggctggcg gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc actcggtgat
    24301 gcagcatcgc gcaattctgt gtggtgtgga gcaacagcgg gcgcgtgagg cgcgtttacg
    24361 tgctgcgcaa cagcaggagg ctgcatgaat attcgccgta aaaaccgctt gtggattgcc
    24421 tgtgctgtgt tggcaggttt ggcgctgacc atcggtctgg tgctgtatgc gctgcgctcg
    24481 aatatcgatc tcttttatac gccgggggaa attctctacg gcaagcgtga aactcagcaa
    24541 atgccggaag tcggtcagcg tctgcgcgtt ggcgggatgg tgatgccggg tagtgtgcag
    24601 cgcgatccca attcgctgaa agtgaccttc accatttacg acgctgaagg ctcagtggat
    24661 gtctcttacg aaggcatttt gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag
    24721 ggcgagctgg aaaaaggcaa tcatatcctc gcgaaagaag tgctggcgaa acacgacgaa
    24781 aactacacgc cgccagaagt tgagaaagcg atggaagcta accaccgtcg cccggcgagt
    24841 gtttataagg acccagcatc atgatgccgg aaattggaaa cgggctgctg tgcctggcgc
    24901 tgggaattgc gctgctgctg tccgtgtatc cgctatgggg cgtggcgcgc ggagatgcgc
    24961 gcatgatggc gtcttcccgc ttgtttgcct ggctgctgtt tatgtctgtg gctggcgcat
    25021 ttctggtgct ggtcaatgct ttcgtggtca acgactttac cgtcacctat gttgccagca
    25081 actccaatac ccagcttccg gtgtggtatc gcgtggcggc tacctggggc gcgcatgaag
    25141 gttcgcttct gctgtgggtg ctgctgatga gcggctggac ctttgcggtg gcgattttta
    25201 gtcagcgtat tccgctggat attgtggccc gtgtactggc gataatgggg atggtcagcg
    25261 tcggcttttt gctgttcatt ctctttacct ctaacccgtt ctcacgcacg ctgctgaact
    25321 tcccaattga agggcgcgat cttaacccgc tgttacagga tccggggctg atcttccatc
    25381 cgcctctgct ttatatgggg tacgtgggtt tctcggtggc gtttgctttt gccattgctt
    25441 ctttgctgag cgggcgtctg gacagcactt atgcgcgttt tactcgcccg tggacgctgg
    25501 cggcgtggat tttcctgacg ctcggcattg tgctcggttc cgcatgggcc tattacgaac
    25561 tcggctgggg gggctggtgg ttctgggatc cggtagaaaa cgcctcgttt atgccgtggc
    25621 tggtggggac tgcgctgatg cactcactgg cggtcactga acaacgcgcc agcttcaaag
    25681 cgtggacatt actgctggca atcagtgcct tctcgttgtg tctgctgggg actttcctgg
    25741 tgcgttccgg cgtgctggta tcggtacacg cgtttgcctc tgatccggca cgcggtatgt
    25801 ttatcctcgc ctttatggta ctggtgattg gtggttcgct gctgctgttt gccgcgcgtg
    25861 gacacaaagt tcgctcacgc gtaaacaatg cgctgtggtc gcgggaatct ctgctgttag
    25921 cgaacaacgt tttgttggtc gccgcgatgc tggtggtatt actggggacg ctgctgccgc
    25981 tggtgcacaa gcaactggga ctgggcagta tttcgattgg cgaaccgttc ttcaacacca
    26041 tgtttacctg gctgatggtg ccgtttgcgc tgctgcttgg tgtcggtcct ctagtgcgct
    26101 gggggcgcga tcgcccacgt aaaatccgca atttattgat tatcgccttc atctcaacgc
    26161 tggtgctgtc gctgctgctg ccgtggctgt tcgaaagcaa agttgtggcg atgacggtgc
    26221 tcggcctggc aatggcctgc tggattgctg tgctggcaat tgcggaagct gcgctgcgta
    26281 tttcacgtgg cacgaaaacc accttcagtt attggggaat ggtggcggct cacctggggc
    26341 tggcagtgac aattgttggt attgccttta gccagaacta tagcgttgag cgtgatgtgc
    26401 gcatgaagtc cggcgatagc gtcgatattc atgaatatcg cttcaccttc cgtgatgtca
    26461 aagaggtgac tggcccgaac tggcgcggcg gtgtggtgac tattggcgta acgcgagagg
    26521 gtaaaccgga aacggtgctg tatgcggaaa aacgttatta caacactgcc gggtcgatga
    26581 tgaccgaagc ggcgattgac ggcggcatca cgcgtgacct gtacgccgcg ctcggtgaag
    26641 agctggaaaa cggcgcgtgg gccgtgcgtc tttactacaa accgtttgtt cgctggattt
    26701 gggcgggcgg gctgatgatg gcgttgggcg gactgctgtg tctgtttgat cctcgctatc
    26761 gtaagcgcgt gagtccgcaa aaaactgcgc cggaggctgt atgaagcgca aagtattgtt
    26821 aattccgttg attatcttcc tggcgattgc cgcggcgctg ctgtggcagc tggcgcgtaa
    26881 tgccgaaggg gatgatccga ccaatctgga atcggcgctc attggcaagc cagttccgaa
    26941 gtttcgcctc gaatcactgg acaatccggg gcagttttat caggcggatg tgctgactca
    27001 gggcaaacct gtgctgctta acgtctgggc tacctggtgt ccgacctgcc gtgcggaaca
    27061 tcaatatctg aatcagcttt ctgcgcaggg cattcgcgtg gtcggcatga actataaaga
    27121 cgatcgccag aaggcaataa gctggctgaa agagctgggc aatccttacg cgctaagcct
    27181 gtttgatggc gatggcatgt tagggctgga tctcggtgtc tatggcgcgc cagaaacgtt
    27241 cctcatcgac ggcaacggca tcattcgcta tcgccatgcg ggcgatctca atcctcgcgt
    27301 ctgggaagaa gagatcaagc cgctgtggga gaaatacagt aaggaggccg cacaatgagg
    27361 tttttattgg gcgtgctgat gctgatgatc tccggctcag cgctggcgac catcgacgtg
    27421 ttgcagttta aggatgaagc gcaggagcag cagttccgcc aactcactga agaactacgc
    27481 tgcccgaaat gccagaacaa cagcattgcc gattccaact cgatgattgc caccgacctg
    27541 cgccagaaag tgtatgaact gatgcaggaa ggtaaaagta aaaaagagat tgtcgattat
    27601 atggtggcgc gttacggcaa cttcgtcact tacgatccgc cgttaacgcc gctgaccgtg
    27661 ctgctgtggg tgcttccggt agtggctatt ggcattggcg gttgggtcat ttacgcccgt
    27721 tcgcggcgtc gggtacgcgt ggtgccggac gcgtttcctg aacaaagcgt gcaggaaggt
    27781 aagcgtgccg gatatattgt ttatctgccg ggtattgtgg tggcgttaat tgtggctggc
    27841 gtcagctact accagactgg caattatcag caggtgaaaa tctggcagca ggccacggca
    27901 caggctccgg cgttgctgga cagggcgctg gatccgaaag ccgatccgct caacgaagaa
    27961 gagatgtcgc gtcttgcgct ggggatgcgt actcaactgc aaaaaaatcc gggagatata
    28021 gaaggctgga ttatgttggg ccgcgttggc atggcgctgg gtaacgccag tatcgccacc
    28081 gatgcatacg ctactgcgta tcgcctcgat ccgaaaaaca gtgatgccgc actgggttat
    28141 gctgaagcgt tgacacgttc atctgatccc aacgacaacc gcctcggtgg tgaactgctg
    28201 cgccagttgg tgagaacgga ccatagcaat atccgtgtgt taagcatgta tgcgtttaat
    28261 gcctttgagc agcagcgatt tggcgaagcc gttgccgcgt gggagatgat gttgaaactc
    28321 ttatctgcca acgatactcg ccgtgcggtg attgaacgta gtatcgcgca ggcgatgcaa
    28381 catttgtcgc cgcaggagag taaataaaaa gaggcccgat ggtgatgcca tcgggctatt
    28441 tttattgtgc cccgcgttgt tgcaggaaca gaatggtggc tgccacgcgt gagcggacat
    28501 tgagtttgcg cagcagattg cgaatatgta cttttactgt ctgctctgaa atattcaaca
    28561 ccgaggcaat ctgtttattt gacagcccct gtgccagctc atgcagaaca tccagctcgc
    28621 gctccgtcag cacgctgaag ggatcttctt ccgcgccaaa catttcacgt tcacgtaagt
    28681 actgattgac gcgttcgcta aagactttgc tgcctttcgc tccagcacga attgcttcca
    28741 gcaatacttc cgggtcgctg tctttcaaca gataaccgtc tgcgcctgcg tctatcagcg
    28801 caaagacatc gctggaggca tctgatacgg tcaggataat aatttgcgcg gtaacgccat
    28861 ccctgcgcaa ggcattgagg gtatccaggc cactcatacc tttcatattg agatccagca
    28921 agatcacgtc gatatccagt ctattcgcca gatcgatcgc gctcgcgccg tcgcccgctt
    28981 cggcgaccac ttcaaagcca ggatcaagct ccagtaactg acgaacaccg cgtcgcataa
    29041 gtggatgatc atccacaatc atcacctgaa aaggcgttgc ttcaggcata gtagtctcct
    29101 ggggttttat tagaatcatt atgtttgagc gtagcgttgg taacgctacc aggcgcgtga
    29161 atgttacccc attttatgta tggacatgta gtgaaaacta caaccttgtc gttcgctttg
    29221 gcatcatttt cattataaag acggaatttt gcatcagctt ctc
//
LOCUS       DAJTNA010000031        29187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTNA010000031 DAJTNA010000000
VERSION     DAJTNA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29187
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.031"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..1748)
                     /locus_tag="OO161_001169"
     CDS             complement(<1..1748)
                     /locus_tag="OO161_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR5462317.1"
                     /translation="MLSLYEKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSR
                     FELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDD
                     NKYYLFSRRVNDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNP
                     AYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLK
                     QGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGN
                     LLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEAL
                     KIREGDAKEIISIKNSIAEMKDAEIERTNKLLSLISYDQESGFIKNMAIIESNNNQYL
                     AVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCREN
                     VQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMF
                     SEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWV
                     SAERGIISPLKFITIA"
     gene            complement(1926..2837)
                     /gene="gsiD"
                     /locus_tag="OO161_001170"
     CDS             complement(1926..2837)
                     /gene="gsiD"
                     /locus_tag="OO161_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5462318.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFGRWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPVFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(2840..3760)
                     /gene="gsiC"
                     /locus_tag="OO161_001171"
     CDS             complement(2840..3760)
                     /gene="gsiC"
                     /locus_tag="OO161_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5462319.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(3778..5316)
                     /gene="gsiB"
                     /locus_tag="OO161_001172"
     CDS             complement(3778..5316)
                     /gene="gsiB"
                     /locus_tag="OO161_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5462320.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGANFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(5336..7207)
                     /gene="gsiA"
                     /locus_tag="OO161_001173"
     CDS             complement(5336..7207)
                     /gene="gsiA"
                     /locus_tag="OO161_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5462321.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRAHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(7194..8159)
                     /gene="iaaA"
                     /locus_tag="OO161_001174"
                     /pseudo
     CDS             complement(7194..8159)
                     /gene="iaaA"
                     /locus_tag="OO161_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            8363..9598
                     /gene="moeA"
                     /locus_tag="OO161_001175"
     CDS             8363..9598
                     /gene="moeA"
                     /locus_tag="OO161_001175"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5462322.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISTGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            9598..10347
                     /gene="moeB"
                     /locus_tag="OO161_001176"
     CDS             9598..10347
                     /gene="moeB"
                     /locus_tag="OO161_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5462323.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(10423..11085)
                     /gene="fsa"
                     /locus_tag="OO161_001177"
     CDS             complement(10423..11085)
                     /gene="fsa"
                     /locus_tag="OO161_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5462324.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            11216..12115
                     /locus_tag="OO161_001178"
     CDS             11216..12115
                     /locus_tag="OO161_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5462325.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHRQKLNPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            12121..14553
                     /locus_tag="OO161_001179"
     CDS             12121..14553
                     /locus_tag="OO161_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5462326.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            14699..15514
                     /gene="ybiV"
                     /locus_tag="OO161_001180"
     CDS             14699..15514
                     /gene="ybiV"
                     /locus_tag="OO161_001180"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5462327.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            15666..16930
                     /locus_tag="OO161_001181"
                     /pseudo
     CDS             15666..16930
                     /locus_tag="OO161_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
     gene            complement(17171..18763)
                     /locus_tag="OO161_001182"
     CDS             complement(17171..18763)
                     /locus_tag="OO161_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5462328.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNDKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18982..19902
                     /gene="ldtB"
                     /locus_tag="OO161_001183"
     CDS             18982..19902
                     /gene="ldtB"
                     /locus_tag="OO161_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5462329.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19961..21079)
                     /locus_tag="OO161_001184"
     CDS             complement(19961..21079)
                     /locus_tag="OO161_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5462330.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSGPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(21076..21543)
                     /gene="mntR"
                     /locus_tag="OO161_001185"
     CDS             complement(21076..21543)
                     /gene="mntR"
                     /locus_tag="OO161_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5462331.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21729..21857
                     /gene="mntS"
                     /locus_tag="OO161_001186"
     CDS             21729..21857
                     /gene="mntS"
                     /locus_tag="OO161_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5462332.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            22129..23712
                     /gene="opgE"
                     /locus_tag="OO161_001187"
     CDS             22129..23712
                     /gene="opgE"
                     /locus_tag="OO161_001187"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5462333.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(23761..24276)
                     /gene="ompX"
                     /locus_tag="OO161_001188"
     CDS             complement(23761..24276)
                     /gene="ompX"
                     /locus_tag="OO161_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5462334.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(24329..24394)
                     /locus_tag="OO161_001189"
     CDS             complement(24329..24394)
                     /locus_tag="OO161_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462335.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            24629..25516
                     /gene="rhtA"
                     /locus_tag="OO161_001190"
     CDS             24629..25516
                     /gene="rhtA"
                     /locus_tag="OO161_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5462336.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            25815..26318
                     /gene="dps"
                     /locus_tag="OO161_001191"
                     /gene_synonym="pexB"
     CDS             25815..26318
                     /gene="dps"
                     /locus_tag="OO161_001191"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5462337.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            26722..27468
                     /gene="glnH"
                     /locus_tag="OO161_001192"
     CDS             26722..27468
                     /gene="glnH"
                     /locus_tag="OO161_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5462338.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGTLKTLRENGTYNEIYKKWFGTEPK"
     gene            27607..28266
                     /gene="glnP"
                     /locus_tag="OO161_001193"
     CDS             27607..28266
                     /gene="glnP"
                     /locus_tag="OO161_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5462339.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            28263..28985
                     /gene="glnQ"
                     /locus_tag="OO161_001194"
     CDS             28263..28985
                     /gene="glnQ"
                     /locus_tag="OO161_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5462340.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQVFLQHVS"
BASE COUNT         7247 a         7768 c         7167 g         7005 t
ORIGIN      
        1 tcagcaatgg taatgaactt cagcggtgaa atgatccccc tctccgcaga aacccaacgg
       61 cacaacgcct ccgcgcccag aatggcgcgg gtgttaatat caacgatcgg ttgatagacg
      121 aggaatagtt cgccaatttc cattgcatgg cgaatattac gcgcaatcca gatattctct
      181 tctatttcgt tgagtttcgc gtcatcacag aaaataaact cggagaacat atggtcacgg
      241 atactggaaa tcgccagttt cagtttttct gcgtaactcc aggcgttttc cccctgcaaa
      301 ggttcacaaa tggcagactt atggatgcgc agattgcggt agccaaagct gctgtcaaaa
      361 tcgtttacca tcgctatttt acgggtaaat gtctgtacat tttcccgaca cagaagtaaa
      421 tagagatcgg cattgaatgt cacgatatcg cagcattgcg catatttctc ggcaattcgc
      481 tgacacaatt tcctgacgat tttattgcgt tcatcaacac caaacaccgc ttccacggct
      541 tccagaccac acagtttgat gatccccaca gccagatact gattattgtt ggactcaata
      601 atcgccatat ttttaataaa accgctttcc tgatcgtaag agatcagtga gagcaattta
      661 tttgtccgtt caatttcggc atctttcatt tccgcgatcg aatttttgat gctgataatc
      721 tctttcgcat cgccttcgcg aattttcaga gcctcgatct gatccgttga gtcgggcaaa
      781 aacgccagcg tattaaccag ctcggtcatt tcagagaatt gccgacggaa cacacgtgtg
      841 gtcatgaaat ataccaccag caacacgata ataatgaacg caaacggtag ataaaaaagt
      901 ttatctgatg cgttcaataa attaccgcca ggataataaa aatagatttc gaacaatgca
      961 ttatttggca ctgctgatgt ctttgcaata tactccgtat cgacagaatc aataatatta
     1021 taattacctt cgctattata aataatcagt ttttcacgag cgagttgatt cttggcaatg
     1081 atctcatgac cttgtttcaa aaatagaact tcatcattag cgctgatttg atcaagatac
     1141 tcgttcaaat accgccagtt taaatcgaca tgaattttat ctaccgctaa caaatcgtga
     1201 tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg atcaaagcaa
     1261 ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca cggtcgtttc
     1321 gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg tccatttact
     1381 tgttcctgat gataaacaac gattttatca ttcaccctcc gtgaaaagag ataatattta
     1441 ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat tttcagaatc
     1501 acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt taatgtcagg
     1561 gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag ttcaaagcgg
     1621 ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat gaaaaaaaga
     1681 ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt ttcgtataaa
     1741 ctcagcatgt taatacattt aacaaaataa ctatctgaac gctgtgccgc tgcaaaaata
     1801 gtcccttgct gcggcttttt aaccaaatta cccgattcta caaaatcagc agaaagttac
     1861 aacacttgct tagggcaata atttcctgtg aggtgattac cctttcaagc aatattcaaa
     1921 cgtaactatc ctttaatttt cggatccagc gcatcgcgta aaccatcgcc caacaaattg
     1981 aacgccagta cggtcagaaa aatagccagg gccggaaaaa cagcgacatg cggcgcgata
     2041 accatatccg ctcgtgcctc attgagcatt gctccccact ctggtgtcgg cggctgcgca
     2101 ccgaggccga gaaatgacag gctggcggca gagataatcg aggtgccaat gcgcatggtg
     2161 aaaaacacca cgatagacga gacggtccca ggcaagatat gacgcaataa aatggtcata
     2221 tcgctggcac caatactgcg tgctgactca ataaaggttt gctgtttcaa caccagcgtg
     2281 ttgccgcgca ccagtcgggc aaacacgggg atggaaaaaa tggcgactgc gataatcacg
     2341 ttagcaatgc cgcttcccaa caccgcaaca acagcgatcg ccagtaaaat acccgggaag
     2401 gcaaacagca catcgcaaat gcgcatgatc agccgatccc accagccttc ataatatcca
     2461 gccaacaagc ccagcaacgt cccgatcgcc gcaccgataa acacggcaaa cacgcccgcc
     2521 gccagcgaga tttgcgcacc aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg
     2581 acgccaaacc agtgctgcaa agaaggtccg ttattcagat tgtcataatc aaaataattt
     2641 tcggcgtcat agggagcgat ccagcgtcca aaaatggcca ccacaatcaa taaaataacg
     2701 aataatgcgg cggtcatcgc catatgctga cggcgaaatc gtcgccagaa ttcatgccac
     2761 ggtgtacgca cctggtcagg tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc
     2821 cagttaaata gtcgcatcct tacttgtacc tgatagccgg gttaatggcg gcgtaaagca
     2881 catccaccac taagttgata agaataaatt ccagcgagaa aagcagaatt tccgcctgaa
     2941 tcaccgggta atcgcgcatt tctacggagt caacgagtaa gcgtccaagt ccgggccagt
     3001 tgaaaacttt ctccaccacg atagaaccac cgagcaaaaa gccaaactgt aagcccatca
     3061 tggtcactac cgggatcatc gcgttacgta gcccgtgttt gaggacaacc caggtttcgc
     3121 tcaccccttt cgccctcgcg gtacgcatat aatcttcgct taaaacatcg acaaacgacg
     3181 cgcgggtaaa gcgcgccatc acggctgcca ctgccgcgcc gagcgtcagg gaggataaaa
     3241 tgtagtgctg ccaactgtct gctcccacgg taggcagcca gcccagttca acggagaata
     3301 cctgaattaa aagcatcccc agcgcaaatg ccggaaacga gatccccgac accgcaatgg
     3361 tcatactcaa tcgatccggc caacgattac gccagacggc ggcgataatt cccgccgcca
     3421 taccaaatat caccgcccag accatactgg ttatggtcag ccacagcgtt ggcataaagc
     3481 ggctggcaat ctcatcggca accggacgac gcgacaccat cgacaggcca aaatccccct
     3541 gcacagcatt gctgatatag tgccagaact ggtgatacag cggctgatcc aaccccagct
     3601 gctgacgcac cagttctata acctgcgcat cagcttcggg cccggcaatc aatcgcgccg
     3661 gatcgccggg cagcatatgc acaaataaaa acaccagcac cgagacgata aacagcgtcg
     3721 gaatcaaccc cagtaagcgt ttgataacgt aattaagcat tccactccct gcgttgctta
     3781 ttgcaaatcc gcgtcttcaa agctgaagcc ggtgtctggc atgatccaaa aaccggtcag
     3841 gtttttactg tgtgccgaca ccagtttttc taccaccagc gggatccacg gcgactcttg
     3901 ccagatgata tcctgcgccg ccttatacaa gcgggtcttt tccgccggat cattagtttt
     3961 cagtgcctga gccaggaagt cgtccacctg tttattgctg taaaatgcgg tattaaacag
     4021 cgtcggtggc cagttttgcg aggcgaacag tggcgatagc gcccagtccg cttcgccggt
     4081 tgaagccgac cagccagtgt agaacatccg cacgccgctc tctttttgcc ctttaccttc
     4141 aacttctgcc gcccgctgtc cggcatccat cgcggtcacc tgggctttaa tcccgacctg
     4201 cgctaactgc tgctgggtaa attgcagcac tttctgcgcg gtgctgtggt tatgtgacga
     4261 ccacagcgtg gtactgaaac cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt
     4321 cactggatcg taaggccacg gtttataact ttgcgcgtag gcgatacttg gcggtaccac
     4381 accagtagct ggcgttgcat agcccgcaaa ggcaactttc accagcgccg gacggttaat
     4441 ggcgtaattc agcgcctcac ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat
     4501 actgatataa cgctgcataa ttgacggact ggccatcaac tcgatatttt tgtttttctc
     4561 cagcagtgcg gcctgctcgt agggaatggg gaaagcaaac tgcgcttcac cggtttgcag
     4621 cattgcagcg cgggtgttgt tatccgccac cggacgccag gttatgctgt ccagtttggg
     4681 caatcctggc tgccagtaac ccgcgaattt tttcaccttc acaaaatcgg tctgattcca
     4741 ggtatccagt tcatacggtc cggttcccac cggatgaaaa ccaatctcct tgccatattt
     4801 ttccagcgct gccggtgaaa tcatcgcggt cgccggatgg gcaagaatat taataaacgc
     4861 tgagaacggc tgtttgaggg taatctttac cgttgtcgga tcgatcgcct ccgttttggc
     4921 aatattctta tacaggttat agcgtttaag atgattcgcc ggatcgctgg cccggtccag
     4981 attcgctttc accgccgcgg cgttgaaatc ggtgccatcc tggaatttaa ttccttcccg
     5041 caatttcacg gtgtaagtaa tgccgtcatc ggaaacggta taactctccg ccagcacgtt
     5101 tttcagtttc atctctttat ccagaccgaa cagcccctgg taaaacgatt tcgctacggc
     5161 ctgagataac gtgtcattcg cgtcatacgg atcgagcgtg gtgaaattcg ctcctaccgc
     5221 caccaccaca tctttggcag cgaatgcaca agatgccatc aacgctgtcg caatgcccag
     5281 cgccactaac ccactacggt gtacagctct tgccatttta ttctccgcct gaatgttatc
     5341 tacgcatgaa tgcgtattct gattgtggtt gtgcgacgta atgccccggc ccgacgcatt
     5401 gcaacgagac ggctgccacc tcttcgccac gcagatgaat attgctggga agatcgtccg
     5461 acagcagtac acgctgcggt cgttgtcggg acggttcagc gaccggaact gccgccagta
     5521 atttacgcgt ataaggatgc tgcgggtttt cgaagaccgc gcgccgtgga ccaatttcaa
     5581 caatttgccc gagatacatc accgccacac gatgactaat ccgctctacc acggccatat
     5641 cgtgggagat aaacagatac gcaatgccga aatcacgctg gagatcgagc aacaagttga
     5701 taatctgccc gcgaatagaa acatccagcg ccgaaacggc ttcgtcggca atgatcactt
     5761 ttggattcaa tgccaacgcg cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact
     5821 catgcgggta acgccaggca tgttcaggta acaggcccac gcgctccagc aaccacgcaa
     5881 cgcgtgcagc cgcgtcttta cctggcaata aaccgtgtgc acgcagcggt tcgataatcg
     5941 aatcaccgat ggtctgacgt gggtccagcg aagcgtaagg gtcctgaaaa ataaactgaa
     6001 tatcccggcg caatgcctga agtttgccag gtgacaaggt atcgattcgc tgaccgttaa
     6061 agataatttc gccgccctgc gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt
     6121 taccgctgcc agactcgccc accagcgata gcgtttcgcc aggccagaga tcaaaactga
     6181 ctttctcaac ggcatgcact tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga
     6241 aacgggtgac aagattacgc actcgtaaaa caggttcgcc atccaccacc gttttctgct
     6301 cgatgggggg ttcctgtttc gctggatgtt caagcgatat caatgggaaa cgtcggggat
     6361 aatctaaccc tttcatcgca ccaagttgcg gaacagcagc taacagcgca cgggtgtaag
     6421 gatgttgcgg tgcatgaaaa atctgttcga cggtacccgt ttccaccgcc tcgccctgat
     6481 acatcaccag tacccgatcg gctatctctg ccaccacgcc catatcgtgc gtgataaaga
     6541 taacgcccat cgacatctct ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa
     6601 tagtgacatc cagcgcggtg gttggctcat cggcaatcag caccgccggg cggcatgaca
     6661 gcgccatcgc aatcatcact cgctggcgca tcccgccaga gagttgatgc ggataacgtg
     6721 aaagaatggt ttgtgcctca ggaatgcgta cctgatccag catccgcttc gcctcgacca
     6781 tcgcttcttc acgactggcg ttctgatgca gacgaattga ttcggcaatc tgttcaccca
     6841 cagtaaatac cgggttcagc gatgtcattg gctcctgaaa tatcatcgcc atatccgcac
     6901 cgcgaacatg gcgcatttgt gcagcgctct gctcgctaag ttcaatcact tcgcgactgc
     6961 gccgctgcaa cagcatttta tcgcactgta ctaaaccgcc cgcctgttcc aacaggcgca
     7021 tcaatgccaa cgcagtcact gacttaccgg agccggattc gccaacaatt gccagcgtct
     7081 caccgcgttg cagactaaaa gagagattgc ggaccgcagc tattttctgc tggtcctgca
     7141 taaaggcaat attcagattt tcaaccgcca gcacattacc ggcatcaagt tcatcactgt
     7201 gtggcaacgg tgtccccttt ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag
     7261 ccccaggcgc gatacattcc ttcggtgtta aacggtagcg cgacattccc ttcatggccg
     7321 atagcgatta agccaccgct accgacaagc gcagggagtt tttccattac tacccgctcg
     7381 caggcttccg cgagacttaa tccgccgtaa tccattaacg cggcgatgtc atatgccgcc
     7441 agcgcgcgga tgaagacttc gcccgtgccg gtacaagaaa ccgccacact ggcgttattg
     7501 gcgtagcatc cggcacccac taagggacta tcgccaactc gtccgggtaa tttattggtc
     7561 attccgcctg tggacgtggc tgccgccaaa ttgccgtcta aatccaactc cacggccccc
     7621 acggtgccca ttttctgttt ttcatccagt ggcgcaccgc tatggtcgag gacggttgcc
     7681 ccttccttgc gcgctgccag tagttgttca taacgcaaag gcgtggagaa aatctccggc
     7741 gagacgcgct ccatgccacg agcaaacgca aaattttctg ccccttagcc aatcatcatc
     7801 acatgcgggc tttgctccat caccacccgg gcggcaagaa ccggattacg cagatgacta
     7861 acgcccgcca ccgcaccggc tttcagggtg ttaccatcca tcacacaggc gtccagttca
     7921 tgggtttcat cacgcgtaaa gacagcgcca attccggcgt taaacagtgg acactcttcc
     7981 agcagacgca ccgcttccgt caccacatcc agcgcacttt cgcccgcttc cagcattttc
     8041 tgcccggttt caacaatggc agacaacgcc tcaatgtagc gtaattcctg ttgcagactc
     8101 atctgcgcgc ggctaattgc acctgcgcca ccatgaattg caatgactgc tttgcccata
     8161 gtattcgtcc attaagggtg gctcgccact tttgctataa atatcagaat ttttatgaat
     8221 tactcattca atttttgaat atagaaagat ggatatggca tgtaaaggca ggagccaacg
     8281 ctacgcccga ggtggcatac agtctgtggc gtttttctga cataataggc aaattcgatt
     8341 ttgcctccgc aggagtgttt tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc
     8401 gcttaatgag atgctttctc gcgtcacccc actgaccgcc caggaaacgc tgccactggt
     8461 acagtgtttt ggtcgtattc tggcgagcga tgtcgtttcg ccgcttgatg ttccggggtt
     8521 tgataactcc gcaatggacg gctacgcggt gcgtttagcc gatattgcct ccgggcaacc
     8581 gctgcccgtt gccggtaaat cctttgccgg tcagccgtat catggtgaat ggcctgcggg
     8641 cacctgcatt cgtattatga ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat
     8701 gcaggagcag actgaacaaa cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg
     8761 gcaaaatatt cgccgtcgcg gtgaagatat ctctacaggt gcggttgttt tcccggcggg
     8821 aactcgcctg actaccgcag agctgccagt aattgcttca ctggggattg ccgaagttcc
     8881 ggtgattcgt aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc agttgcccgg
     8941 tcagccgctg ggcgacggcc aaatctacga caccaaccgt ctcgccgtac acctgatgtt
     9001 agaacagctg ggttgcgagg taatcaactt agggattatc cgcgacgatc cccatgccct
     9061 gcgcgccgca tttattgaag ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt
     9121 ttcagtgggt gaagcggatt acaccaaaac gattcttgaa gagctggggg agatcgcctt
     9181 ctggaagctg gcgattaaac caggtaaacc gttcgcgttc ggtaaactca gcaatagctg
     9241 gttctgcggc ctgccgggca acccggtttc agcgacgctg accttctatc aactggtaca
     9301 gcctttgctg gcaaaactaa gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt
     9361 acgcacagcg tcccgcctga aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct
     9421 gcaacgcaac gccgacggtg aacttgaagt gacgaccacc ggacatcagg gttcacatat
     9481 atttagctcc tttagtctcg gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt
     9541 ggacgtgggc gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg
     9601 gcggaactca gcgatcagga gatgctgcgc tacaaccggc aaatcattct gcgcggtttt
     9661 gattttgacg gtcaggaggc gctaaaagat tctcgtgtgc tgatcgtcgg cctcggtgga
     9721 ctcggctgcg cagcttcgca gtatctggca agcgccggtg tcggcaacct gacgctgctc
     9781 gacttcgaca cggtttcgct ctcgaatctg caacgccaga cactgcacag tgatgccacg
     9841 gtcgggcaac cgaaggtgga atccgcccgt gacgccctga cgcggatcaa cccacatatc
     9901 gcgattacgc cagtcaatgc actgctagat gacgcagaac ttgcagcatt gattgctgaa
     9961 cacgatctgg tgctcgactg tacggataac gttgcggtac gtaatcaact gaacgcaggc
    10021 tgttttgccg cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc
    10081 acggtcttca cttatcagga cggcgaaccg tgctatcgct gccttagccg tttgtttggc
    10141 gagaacgcgt taacctgcgt agaagcaggc gtgatggcac cgttgatcgg cgtaattggt
    10201 tcgttgcagg caatggaagc gatcaaactg ctggcaggtt atggcaaacc ggccagcggg
    10261 aaaatcgtca tgtatgatgc gatgacctgt cagtttcgcg aaatgaaact gatgcgtaat
    10321 ccggggtgtg aggtgtgcgg gcagtaattg ccacctccag gccagataag acgcggcact
    10381 ccgccacgtc atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc
    10441 aaacgctccc agccagtcct gttcaaactt cgccacagcg gcatcaaccg ccggatagct
    10501 aatcatctgt tgtgccacat ccagtggcag cgtaattgat tcacatcctg ccagtaagca
    10561 gtccagcgcc tgacgcgggt ttttgaaact cgctgccagc actttcgcct gcggcgcatg
    10621 cattttcaat aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc
    10681 aatacgatta acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcagcccttg
    10741 tgctgcgcca tataccgcgg ttcccagcgt tggaatccct tccgctttta acatcttaat
    10801 agctgccagc ccctcggcgg tcaccggaac tttcaccacg atatccgcaa taatagaacg
    10861 caatttacgt gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag
    10921 acgcccctga ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt
    10981 acccgcggcg ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag
    11041 cgccttcacc gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat
    11101 gtactttttg tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc
    11161 aatccaactt tcattcaaaa gtgttttaat gtttgaacga aatagagagg caactatgat
    11221 tttcaatatt cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct
    11281 taaaggctgt tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca
    11341 ggatctgctg tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggcagc
    11401 gccggaagtg attgagcgcg cattgaatgg tttgcttatt catcggcaaa agttaaaccc
    11461 ggagcatctg acggcgctaa ccgactgctg cccgacacag gcgttaaccg tgtgtgggga
    11521 agtgaaaagc gttgaggaga tcatggcgac cgttctgcgc gataaaccgt tttacgatcg
    11581 cagcggcggc ggcttaacgc tttcgggtgg tgaacccttt atgcagccgg aaatagcgac
    11641 ggcgctactg caagcctgcc acgaggcagg cattcatact gcggtagaaa cctgtctgca
    11701 tgtgccgtgg aaatatatcg ccccttctct gccctatatc gatctgtttc ttgccgattt
    11761 aaaacacatt gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccaaggtgct
    11821 ggataacctg aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat
    11881 tcagggcttt aatgccgacg aaacctctgt aaaagccatt accgattttg ccgccgacga
    11941 gctgcacgtt ggcgaaattc attttctgcc ctaccacacg ctgggcatca acaaatatca
    12001 cttacttaat ctgccctatg acgccccgga aaaaccgctt gatgcgccag acctgctcga
    12061 ctttgcccag cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc
    12121 atgaccacac tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg
    12181 gtgcatattg tgaaaccgcc ggtctgtacc gagcgcgcgc agcactatac cgagatgtat
    12241 caacaacatc tcgataagcc gatcccggta cgtcgcgcgc tggctctggc gcatcacctg
    12301 gcgaatcgca ccatctggat caagcacgat gagttgatca tcggcaacca ggcaagcgag
    12361 gttcgcgctg cgccgatctt cccggaatat accgtctcgt ggatcgaaaa agagattgat
    12421 gatctggcag atcgtcccgg tgctggtttt gcggtgagcg aagagaacaa acgcgttctg
    12481 catgaagtgt gcccgtggtg gcgcggtcag acggtacagg atcgctgcta cggcatgttt
    12541 accgatgagc aaaaaggtct gctggcgacc ggaatcatta aagcggaagg caatatgacc
    12601 tccggcgatg cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt
    12661 ctgcgcgaga aagtagcgga acgtcgctcg cgcatcaatc tgacggtact ggaagatttg
    12721 cacggcgaac agttcctgaa agcgattgat atcgtgctgg tggcagtcag cgaacacatt
    12781 gaacgttttg cagccctggc gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc
    12841 gatgaactgc tggcgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact
    12901 ttctggcagg cgctgcaact gtgttacttc atccagttga tattgcagat cgaatctaac
    12961 ggtcactcgg tatcgtttgg tcgtatggac cagtatctct acccgtacta tcgccgcgac
    13021 gttgaactca accagacact ggatcgcgaa cacgccatcg aattgctgca tagctgctgg
    13081 ctgaaactgc tggaagtgaa caagatccgc tccggctcac actcaaaagc ctctgcggga
    13141 agtcctctgt atcagaacgt caccattggc ggacaaactc tggttgatgg tcaaccaatg
    13201 gacgcggtga atccactctc ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact
    13261 cagcctaacc tcagcgtgcg ttaccatgca ggaatgagta atgatttcct cgacgcctgc
    13321 gtacaggtga tccgttgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc
    13381 ccggaattta ttaaactcgg tattgaaccg caggacgctt acgactacgc agcgattggt
    13441 tgtatcgaaa ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc
    13501 aacttcgccc gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac cagcggcaac
    13561 gtgttcctgc cacaagaaaa agcgttgtcg gcaggcaact ttaacaattt cgatgaagta
    13621 atggacgcgt gggatacgca aatccgttat tacacgcgta aatcaatcga aatcgaatat
    13681 gtcgtcgaca ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat
    13741 gactgtattg agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct
    13801 ggcctgcagg ttggcattgc caacctcggc aacagcctgg cggcagtgaa gaaactggta
    13861 tttgaacagg gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat
    13921 ggcctgactc atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac
    13981 gacgatgata ctgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg
    14041 aaacagtacc ataatccgcg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt
    14101 acgtcatcta tctccgctaa cgtaccgttt ggtgcgcaaa ctatggcaac gccggacggg
    14161 cgtaaagccc acaccccgct ggcagaaggc gctagtccgg cctctggtac tgaccatctc
    14221 ggccctactg cggtcattgg ctcggtgggt aaactgccta cggcagcgat tctcggcggc
    14281 gtgttgctca accagaaact gaatccggca acgctggaga acgaatctga caagcagaag
    14341 ctgatgatcc tgctgcgcac cttcttcgag gtgcataaag gctggcatat tcagtacaac
    14401 atcgtttccc gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg
    14461 gtagtgcgtg tcgcgggtta ttccgccttc ttcaccgcgc tctctccaga cgctcaggac
    14521 gatatcatcg ctcgtactga acatatgctg taatctcttc ggcccggctg cggcgtcgcc
    14581 gggctaaatc attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc
    14641 tctgcgtcac attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat
    14701 gagcgtaaaa gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta
    14761 caaccaacca cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttgagttcgt
    14821 tgtcgccagc ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga
    14881 gatctctttt gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca
    14941 cggcgaactg acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca
    15001 actcaatttt gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc
    15061 atttgtcgca ctgatggcaa aacactacca tcgcctgaaa gctgtaaaag attatcagga
    15121 gattgacgac gtactgttca agttttcgct caacctgcca gatgaacaaa tcccgttagt
    15181 gatcgacaaa ctgcacatag cgttcgatgg cattatgaag cccgtcacca gtggttttgg
    15241 ctttatcgac ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa
    15301 acgctgggat ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga
    15361 gatgctgaaa atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca
    15421 aatcgcccgt tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc
    15481 ggtgctggat aacacatccc cttttaacag ctgacctctc taccggagcc gtgcgctccg
    15541 gttttccctt ctttttacat cctgtacatc acatcaaatc attaaacttg cattaactta
    15601 tttataactt atattgtcac atgcaaatca taatggtttc gaatgaaagt taatgagagg
    15661 tggttatggc ttctactttt accagcgaca cattgccagc cgatcacaaa gcagctatcc
    15721 gtcagatgaa gcacgcgctg cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc
    15781 taagcgatga cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggtgatg
    15841 ccgtctggcc ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc
    15901 gcgaacagat taaacgtcgc ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag
    15961 cgctaggctg ggatcagtcg atgctggact atctggaccg caaccgcttt gacgaggtct
    16021 acaaaggccc cggcgataat ttcttcggga cgctcagcgc ttcacgtccc gagatttacc
    16081 ccatctactg gttgcagacg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc
    16141 agtcgtttct caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg
    16201 atgtgagcgt catctaccct gaccgtatcc gccgccgtcc gcccggaacg acctccaaag
    16261 gtctggagcg cataccgact ccggggcgct ggaacgctgg ctgcttctag cgtatcagcg
    16321 cgttttcgcc aacgtcttta atggcaatct ggcgcaatat gatccctggc atgcggcaca
    16381 tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac
    16441 attccagggc tggacagcgc tctctgatat gctgcctggt caggggttgc tgcacgttgt
    16501 gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga
    16561 ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc
    16621 actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg
    16681 gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt
    16741 gatgtacatt cctgcggcac cgatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa
    16801 ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa
    16861 ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggcat
    16921 ggatgtttga gagttaatgg aagtgaattg atgtgggatt aagaaaatcg attcgacgtt
    16981 actcagattg ctgacaagtg cgctttctta atgcctgatg cggcgttaac gccttatctg
    17041 gcctacaaag tcctgcagat tcaatatatt acaggtactg cgtaggcctg ataagcgtag
    17101 cgcatcaggc tattttgcat ttgtcatcag ccgccgtcac cccgacagta tcggggtgac
    17161 ggataatcac ttactcgatc cctttactac gcaggtaatc ttcgtaatta ccgctaaagt
    17221 cgatcacgcg ttccggggtg atttccagaa tgcgggtcgc cagggagctt acgaactcac
    17281 ggtcgtgtga aacaaagatc agcgtgccct gatacagttc cagcgccatg ttcagtgact
    17341 cgatggattc catatccagg tggttggtcg gttcgtccat gatcagaatg ttcggcttct
    17401 gcatcattaa cttaccaaac agcatccgcc ctttttcccc accggaaagc actttagctg
    17461 gctttttgat gtcgtcctgg ctgaacagca aacgaccgag aatgctgcgt accgcctgct
    17521 cgtcatcgcc ttcctgcttc cactgactca tccattcgaa cacggtcaga tcattttcaa
    17581 actcatattc gtggtcctgg gcgtagtaac caatgcgcgc gttttcagac cattttacgg
    17641 tgccgctgtc cggttgcaga tcgcccacca gcgttttcat cagcgttgat ttaccgacgc
    17701 cgttggtacc cagtaccgca agtttttcac cgacctccag cagcaggttg agatttttaa
    17761 acagtggacc gttatcaaac cctttggtca gaccttccac ttccagcgcg ttacggaaca
    17821 gtttcttatc ctgttcaaaa cggatgaacg ggttctgacg gctggaggct ttcacctctt
    17881 ccagtttgat tttatcaatc tggcgcgcgc gcgaagttgc ctggcgagat ttcgaggcgt
    17941 tggcgctaaa gcggctaacg aaagattgca actcagcaat ctgcgctttc ttcttgtcgt
    18001 tatcggccag cagacgttca cgcgcctggg tcgccgccgt catgtactca tcgtagttac
    18061 ccggataaac gcgcagctcg ccgtaatcca gatccgccat gtgggtacag accatgttaa
    18121 ggaagtgacg gtcgtgcgag atgatgatca tggtgctgtc acgctcgttc agcacctgtt
    18181 ccagccagcg aatggtgtcg atgtcgaggt tgttggtcgg ttcgtcgagc aggagaatat
    18241 ccggatcagc aaacagcgcc tgcgccagaa gcacacgcag cttccagcca ggagcaactt
    18301 cactcatcgg gccgtagtgc tgttccactg gaattcccac gccaagcaac aattcaccgg
    18361 cgcgagcttc cgcagagtaa ccgtccattt cgccgtattt aacttccaga tcggccactt
    18421 tatagccgtc ttcctcactc atttccggca aagcatagat gcggtcacgc tcctgcttca
    18481 cttcccacaa ctctttatgc cccatgatca ccgtatccag cacagtgaac tcttcaaagg
    18541 caaactgatc ctgacgcagc ttaccaatgc gctcgttggg atcgagggaa acgttaccca
    18601 aagtcggctc taagtcgccg ccgaggatct tcataaaggt ggatttacca ctaccgttcg
    18661 cgccaatcag gccgtaacgg ttgccgccgc caaatttgac ggaaatgttt tcaaacaacg
    18721 gcttactgcc gaactgcatg gtgacgttac tggaaactaa cacaggcgta tcctgaaaag
    18781 agatatgaca aaccgcgcat tatgccataa gtctgaaaga agatcctgct cctcgcttaa
    18841 ggtctacact caagccaaag gcaaaaaatg tgatttcgta cacatctgat ttcactgtga
    18901 gctggaatga acttataatg cgcttccaat actctaatat tctcaaccca atggcctgcc
    18961 aggcacaaaa tctcgcttaa catgaatatg aaattgaaaa cattattcgc agcggccttc
    19021 gctgttgtcg gcttttgcag taccgcctct gcggtaactt atcctctgcc aaccgacggg
    19081 agtcgcctgg ttggtcagaa tcaggtgatc accattcctg aaggtaacac tcagccgctg
    19141 gagtattttg ccgcggagta ccagatgggg ctttccaata tgatggaagc gaacccgggt
    19201 gtggatacct tcctgccgaa aggcggtact gtactgaaca ttccgcagca gctgatcctg
    19261 ccggataccg ttcatgaagg catcgtcatt aacagtgcag agatgcgtct gtattactat
    19321 ccgaaaggga ccaacaccgt tatcgtgctg ccgatcggca ttggtcagtt aggcaaagat
    19381 acgcctatca actggaccac caaagttgag cgtaagaaag caggcccgac ctggacgccg
    19441 accgccaaaa tgcacgcaga gtaccgcgct gcgggcgaac cgcttccggc tgtcgttccg
    19501 gcaggtccgg ataacccgat gggtctgtat gcactctaca tcggtcgcct gtatgctatc
    19561 catggcacca acgccaactt cggtatcggc ctgcgtgtaa gtcatggttg tgtgcgtctg
    19621 cgtaacgaag acatcaaatt cctgttcgag aaagtaccgg tcggtacccg cgtacagttt
    19681 attgatgagc cggtaaaagc gaccaccgag ccagacggca gccgttatat tgaagtccat
    19741 aacccgctgt ctaccaccga agcccagttt gaaggtcagg aaattgtgcc aattaccctg
    19801 acgaagagcg tgcagacagt gaccggtcag ccagatgttg accaggttgt tcttgatgaa
    19861 gcgattaaaa accgctccgg gatgccggtt cgtctgaatt aatcttcaaa acttaaagca
    19921 aaaggcggac tcataatccg ccttttttat ttgccagacc ctagttggcc gggagtataa
    19981 ctaacaaaac atatcccacc aacgccgccc acaacagcat cggtattgaa tagagatgga
    20041 aacgccacca gatgcggcga tcgttcgcca tacgtagcgc aatcaaactt gccagcgatc
    20101 cgggtaataa cccaaagcca cctacgttta ccgcccatgc cagtaataaa gacggcggca
    20161 catagttcag caacaatatg gtactcggca cgttactgat cacctgcgat aaaccgattg
    20221 ccgttaacca taacccgggt tcagatagat gactcacgtt acccaacacg ccttgcaacg
    20281 ctggaagctg ggtcagtaaa tggacgtcga taaacatcgc cataaacacc agcagcagcg
    20341 tccagtccac actgagcacc acgcgacgtg ccagcagcgc aaagcctgcc gccacaatca
    20401 ccagtcccca cagctcttgt ttgaactcca gcgccgttag aaagacgata tacagcccca
    20461 gacaactcca caccagccgc ggtttccact ccggtgtttg cacccccgta tggtattgca
    20521 gcacctttcc agggaaacaa caccagcaca ggagcaggag cgtcagcatc attgcgccag
    20581 ccagcggtgc catttgggca ataaatccgg caaacgaaag accagaacgt ccccagataa
    20641 gaatattttg cgggttgcca attggcgtca gtagcgagcc agcattgact gccagcgcct
    20701 caaaaataat cagccgatta accgggatct cacacagtct ttttagcgtg atagtcagcg
    20761 gaacaacaat aaacagcgcg acatcgttgg tcagaaaggt agaaagcagc gccgccgcca
    20821 gcaccataaa catcgccagc cgacgctccg tagcaaagcg gcgcaccatt ttgcgcccca
    20881 gcacatcaaa ataaccgctt aactccacac ctttggtcag cagcatcagg ccgcttaagg
    20941 tgatgatggt gtgccagtcg atagcagcag gcccggattt cggtgcaaag ggcacgaaaa
    21001 agcttaatcc gataccaaca agaattaata actgaaaaaa acgatcgcct tgcagcgtgc
    21061 gtaaaaaagg caggctcatt tggcaccgtg tttctgggta aacaaacgaa aagcgtcgag
    21121 cgtctcttca ctaacatggt gctccatgcc ttccgcgtcg cgacgggcga tttccggact
    21181 gacgcccaac accaacaaga aattttcgac tatctgatgt cgctcgcggc tttcctgcgc
    21241 cagcttctct ccttctgccg ttaaaaacac gccacgccag gggatcattt caatcagccc
    21301 catggttgcc agccgcttaa gcattttagc caccgtcggt tgcgaaactc ccagacgagc
    21361 agccatgtcc acctgacgag cttcccccac ttccctgatc aagtcagaaa tcagctcaac
    21421 gtaatcatca ataagctcgc gccgatgcgc ctctctgacc tggcggaacc cttcaacgtg
    21481 ctcttccacg ttcactaact gcgtcacttt ttttgctgtt ggcgtacctg cgcgacgact
    21541 cattgtgctt cctcattatg gtgacgcata catagcgttc tcatttcgtg cgcacattgt
    21601 aaaccagagt tgcgaaggta caaaaaatta acgttttagc aatagctata taatatagcc
    21661 tgtgctatat ctgtatgtag tgcaatcatc cctcaaggat cgacgggatt aacaagtcag
    21721 gaggtcttat gaatgagttc aagaggtgta tgcgcgtgtt tagtcattct ccctttaaag
    21781 tacggttaat actgctctct atgttgtgcg atatggtcaa caacaaaccg cagcaagata
    21841 aaccttccga taaatagcgg cgtcgcggta cgccgcttca ctcctgcttt catgcaggca
    21901 taacgcgttt tggtctgaaa aaccccactt tttgtcggat ttgcaatccc cttcgcaaaa
    21961 gatttgttcg tcagtagttg acctgaacgg cggctcgctc tatcttcttg cagccctgcg
    22021 tatattgcgg ctcgcggatg cggacccctt tccactcttc acgcactctt gcaggtattg
    22081 acccttgacg ccagggtaag cacatggcgt ttgttacgat agtggcgtat gaatttaacc
    22141 ctcaaagaat cgcttgttac ccgtagccgg gtatttagcc cgtggactgc gttctacttt
    22201 ttacagtcgc tattaattaa cctcggctta ggttacccct tcagtttgct ctacaccgct
    22261 gcgtttacgg ctattttgct tttgctatgg cgaacattgc ctcgcgtaca aaaagttctg
    22321 gtcggtgtca gttcgctggt ggcggcttgt tatttccctt ttgctcaggc ctacggcgcg
    22381 cctaatttca atacattgct ggcattgcac tccaccaata tggaagagtc gaccgaaatc
    22441 ctgacgattt ttccgtggta cagctacctg gtcggcttat ttatttttgc gctcggcgta
    22501 atagcaatca ggcgaaaaaa agagaatgaa aaagcgcgct ggaatacctt cgacagcctg
    22561 tgtctggtat tcagtgtggc gacatttttt gttgctcccg tgcaaaacct ggcctggggt
    22621 ggcgtattta aactgaaaga tactggctat ccggtatttc gttttgctaa ggatgtcatc
    22681 gtcaataata acgaggtgat tgaagagcaa gaacggatgg caaaactttc cggaatgaaa
    22741 gatacctgga cggtcactgc cgttaagccg aagtatcaga cctatgtggt ggtgatcggt
    22801 gaaagcgcgc gtcgcgatgc cctcggtgcc tttggcggtc actgggacaa taccccgttt
    22861 gccagcagcg ttaacggttt gatatttgct gactacattg ccgccagtgg ctccacgcag
    22921 aaatcgcttg gcttaacgct caatcgcgta gtcgatggca aaccacagtt tcaggataac
    22981 tttgtcaccc tggcaaatcg cgcgggcttc cagacctggt ggttttccaa ccagggccaa
    23041 atcggcgaat acgatactgc tatcgccagc atcgccaaac gtgcagatga agtgtacttc
    23101 ctgaaagaag gtaattttga agcagataaa aacacgaaag acgaagcgtt actggatatg
    23161 accgctcaag tgctggcgca agagcactcg caaccgcagc tgattgttct gcatctgatg
    23221 ggctcgcatc cgcaggcctg cgacaggaca caaggcaaat acgaaacctt tgtgcaatcg
    23281 aaagaaacgt cgtgctatct ctataccatg acgcaaacgg acgatttact gcgcaagctg
    23341 tacgatcagt tacgcaacag cggcagcagc ttctctctgg tttacttttc tgaccacggt
    23401 ctggccttta aagagcgcgg caaagacgtg caataccttg cccatgatga taaatatcag
    23461 caaaatttcc aggtgccttt tatggtcatt tccagcgacg ataaagcgca tcgggtgatt
    23521 aaagcccgcc gctcagccaa tgacttctta ggctttttct cgcagtggac gggaattaaa
    23581 gcgaaggaaa taaatatcaa atacccgttt atatctgaga agaaagccgg gccgatatac
    23641 atcaccaact tccagttaca gaaggtggat tacaaccatc tcggaaccga tattttcgac
    23701 ccgaaacctt aaaaacaaaa atccgccccg agaggcggat tttttatatc accaaagtga
    23761 ttagaagcgg taaccaacac cggcaatcca ggtgcctacg tcaacgctac gaatacggct
    23821 ctgctcgtaa gagaagtcca gagcaacgtt ttccatcggg ttgaactgca gaccagcacc
    23881 gtaggagaaa ccgtagtcgc tggtgtcgtg tttgtaggtc gggtattcag tggtctggaa
    23941 tttaccataa cccacaccca ctacaccgta gatgcttgcc cagtcgttaa tgcggtaagc
    24001 cggaccagca gtgatgccgt agtactggtt tttgttgtag tcaccagagc ttgcagtacg
    24061 acttttctcg gtgtaagtga aagaaccgat cacacccagc gggctgttgt cttcttcata
    24121 gcggtatttc aggttgaaac cgcccatttt gttcatttgg ccctgagcgt cgctctgtgc
    24181 gtaaccgcca gttacggtag aagtcgcagc tacggaagta cctgcggtga aagccagaac
    24241 tgcggccagt gctgaaagac atgcaatttt tttcataacc acctcaaatg tgattcaaat
    24301 aagtcctaag ttttaaatat atcaaaaatt aatgggaaac tcttcgcgat ttgtgatgtc
    24361 taacgggcca tttcatgtaa cagaacgttt ccatacaccg ctatccatct aaatttaaat
    24421 cactttttca gagaactgcg taagtattac gcatgttttc cctgtcattc atccagatta
    24481 ttcctaatca ccagactaat gattccatca atcctggcgc attttagtca aaacggggga
    24541 aaattttttc aacaaatgct caaccagcat tgggtatatc cagtacactc cacgctttac
    24601 ttaagtctag atatttgtgg gagaaaggat gcctggttca ttacgtaaaa tgccggtctg
    24661 gttaccaata gtcatattgc tcgttgccat ggcgtctatt cagggtggag cctcgttagc
    24721 taagtcactt tttcctctgg tgggcgcacc gggtgtcact gcgctgcgtc tggcattagg
    24781 aacgctgatc ctcatcgcgt tctttaagcc atggcgactg cgctttgcca aagagcaacg
    24841 gttaccgctg ttgttttacg gcgtttcgct gggtgggatg aattatcttt tttatctttc
    24901 tattcagaca gtaccgctgg gtattgcggt ggcgctggag ttcaccggac cactggcggt
    24961 ggcgctgttc tcttctcgtc gcccggtaga tttcgtctgg gttgtgctgg cggttcttgg
    25021 tctgtggttc ctgctaccgc tggggcaaga cgtttcccat gtcgatttaa ccggctgtgc
    25081 gctggcactg ggggccgggg cttgttgggc tatttacatt ttaagtgggc aacgcgcagg
    25141 agcggaacat ggccctgcga cggtggcaat tggttcgttg attgcagcgt taattttcgt
    25201 gccaattgga gcgcttcagg ctggtgaagc actctggcac tggtcggtta ttccattggg
    25261 tctggctgtc gctattctct cgaccgctct gccttattcg ctggaaatga ttgccctcac
    25321 ccgtttgcca acacggacat ttggtacgct gatgagcatg gaaccggcgc tggctgccgt
    25381 ttccgggatg attttcctcg gagaaacact gacacccata cagctgctgg cgctcggcgc
    25441 tatcatcgcc gcttcaatgg ggtctacgct gacagtacgc aaagagagca aaataaaaga
    25501 attagacatt aattaaattt acatttctgc atggttatgc ataaccatgc agaatttctc
    25561 gctacttttc ctctacaccg tctttatata tcgaattatg caaaagcata tttattccga
    25621 aaattcctgg cgagcagata aataagaatt gttcttatca atatatctaa ctcattgaat
    25681 ctttattagt tttgtttttc acgcttgtta ccactattag tgtgatagga acagccagaa
    25741 tagcggaaca catagccggt gctatactta gtctcgttaa ttactgggac ataacatcaa
    25801 gaggatatga aattatgagt accgctaaat tagttaaatc aaaagcgacc aatctgcttt
    25861 atacccgcaa cgatgtctcc gacagcgaga aaaaagcaac agtagagttg ctgaatcgcc
    25921 aggttatcca gtttattgat ctttctttga ttaccaaaca agcgcactgg aacatgcgcg
    25981 gcgctaactt cattgccgta catgaaatgc tggatggctt ccgcaccgca ctgatcgatc
    26041 atctggatac catggcagaa cgtgcagtgc agctgggcgg tgtagctctg gggaccactc
    26101 aagttatcaa cagcaaaacc ccgctgaaaa gttacccgct ggacatccac aacgttcagg
    26161 atcacctgaa agaactggct gaccgttacg caatcgtcgc taatgacgta cgcaaagcga
    26221 ttggcgaagc gaaagatgac gacaccgcag atatcctgac cgccgcgtct cgcgacctgg
    26281 ataaattcct gtggtttatc gagtctaaca tcgaataaat ccatcgctga tggtgcagaa
    26341 ctttagtacc cgataaaagc ggcttcctga caggaggccg ttttgttttg cagcccacct
    26401 caacgcactt atttagtgca tccatctgct atctccagct gattaagtaa attttttgta
    26461 tccacatcat cacacaatcg ttacataaag attgtttttt catcaggttt tacgctaaat
    26521 aatcactgtg ttgagtgcac aattttagcg caccagattg gtgccccaga atggtgcatc
    26581 ttcagggtat tgccctataa atcgtgcatc acgtttttgc cgcatctcta aaaatcaatg
    26641 agttgcaaaa ctggcacgat tttttcatat atgtgaatgt cacgcagggg atcgtcccgt
    26701 ggatagaaaa aaggaaatgc tatgaagtct gtattaaaag tttcactggc tgcactgacc
    26761 ctggtttttg cggtttcttc tcatgccgcg gataaaaaat tagttgtcgc gacggatacc
    26821 gccttcgttc cgtttgaatt taaacagggc gataaatatg tgggctttga cgttgatctg
    26881 tgggctgcca tcgctaaaga gctgaagctg gattacgaac tgaagccgat ggatttcagt
    26941 gggatcattc cggcactgca aaccaaaaac gtcgatctgg ctctggcggg cattaccatc
    27001 accgacgagc gtaaaaaagc gatcgatttc tctgacggct actacaaaag cggcctgtta
    27061 gtgatggtga aagctaacaa taacgatgtg aaaagcgtga aagatctcga cgggaaagtg
    27121 gttgctgtga agagcggtac tggctccgtt gattacgcga aagcaaacat caaaactaaa
    27181 gatctgcgtc agttcccgaa catcgataac gcctatatgg aactgggcac caaccgcgca
    27241 gacgccgttc tgcacgatac gccaaacatt ctgtacttca tcaaaaccgc cggtaacggt
    27301 cagttcaaag cggtaggtga ctctctggaa gcgcagcaat acggtattgc gttcccgaaa
    27361 ggtagcgacg agctgcgtga caaagtcaac ggcacgttga aaaccctgcg cgagaacgga
    27421 acttacaacg aaatctacaa aaaatggttc ggtactgaac cgaaataata acgctacacc
    27481 tgtaaagcgc actggcagtt ccctctcccc tctggggaga ggattagggt gaggggcgca
    27541 aacccgctcc ggggccatta attaccctga atttgattat ttacaccacg gtaacaggaa
    27601 caacatatgc agtttgactg gagtgccatc tggcctgcca ttccgcttct gattgaaggt
    27661 gccaaaatga ccctgtggat ttcggtcctc ggtctggctg gcggtctggt aatcggattg
    27721 ctggcaggtt ttgcacgcac cttcggtggt tggatagcca accacgtcgc gctggtcttt
    27781 attgaagtga tccgcggcac acctatcgtc gtccaggtga tgtttatcta tttcgccctg
    27841 ccgatggcgt ttaacgactt acgcatcgac ccatttactg cggcggtggt caccatcatg
    27901 atcaactccg gcgcgtacat tgcggaaatc acgcgtggtg cggtgctgtc tattcacaaa
    27961 ggttttcgtg aagcaggact ggcgctcggt ctttcacgtt gggaaaccat tcgctacgtc
    28021 attttaccgc tggcactgcg tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc
    28081 attaaagaca cctcgctgtt tattgttatc ggcgtggcgg aactgacccg tcaggggcaa
    28141 gaaattattg ccggtaactt ccgcgccctt gagatctgga gcgccgtggc ggtgttctat
    28201 ctgattatta ccctggtgct gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc
    28261 ctgtgattga atttaaaaac gtctccaagc actttggccc aacccaggtg ctgcacaata
    28321 tcgatttgaa cattgcccag ggcgaagtcg tggtgattat cgggccgtcc ggttccggta
    28381 aatcgaccct gctgcgctgc atcaacaaac tggaagaaat cacctccggc gatctgattg
    28441 tcgatggcct gaaggttaac gatccgaaag ttgacgagcg cctgattcgc caggaagcag
    28501 gtatggtgtt ccagcagttt tacctcttcc cgcatctgac ggcgctggaa aacgtcatgt
    28561 ttggtccgct acgcgtgcgt ggcgcgaaca aagaagaggc ggaaaaactg gcgcgtgagc
    28621 tgctggcgaa agtcggtctg gcagaacgtg cacatcacta cccttccgaa ctttctggtg
    28681 gtcaacagca gcgtgtggcg attgcccgcg cgctggcggt gaagccgaaa atgatgctgt
    28741 ttgatgaacc gacttccgct cttgacccgg aactgcgcca tgaagtgctg aaggttatgc
    28801 aggatctggc tgaagaaggg atgacgatgg tgatcgtgac ccacgaaatc ggttttgccg
    28861 agaaagtagc ttcgcggctg atctttatcg acaaaggccg gattgcggaa gatggcaatc
    28921 cgcaggtgtt gatcaagaac ccgccgagcc agcgcttgca ggtatttttg cagcacgtct
    28981 cttaattaga cacattgcct gatgcgctaa gcttatcagg cctacaggat atctggcaac
    29041 ttattaaaat tgcatgaact tgtaggacgg ataaggcgtt cacgccgcat ccggcaaaaa
    29101 agcccgcacg ttgtcagcaa tctgcttaat atcccttcct ccctttcacc cgaaagggag
    29161 gcacaccaga ttcctctcat ttaaaat
//
LOCUS       DAJTNA010000032        28636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTNA010000032 DAJTNA010000000
VERSION     DAJTNA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28636
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.032"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            46..957
                     /locus_tag="OO161_001195"
                     /pseudo
     CDS             46..957
                     /locus_tag="OO161_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1000..1860)
                     /gene="rsmI"
                     /locus_tag="OO161_001196"
     CDS             complement(1000..1860)
                     /gene="rsmI"
                     /locus_tag="OO161_001196"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5462341.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1924..3960
                     /gene="lpoA"
                     /locus_tag="OO161_001197"
     CDS             1924..3960
                     /gene="lpoA"
                     /locus_tag="OO161_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5462342.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPAQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSHVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3918..4313
                     /locus_tag="OO161_001198"
     CDS             3918..4313
                     /locus_tag="OO161_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5462343.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4333..4923
                     /gene="diaA"
                     /locus_tag="OO161_001199"
     CDS             4333..4923
                     /gene="diaA"
                     /locus_tag="OO161_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5462344.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4933..5508
                     /gene="yraP"
                     /locus_tag="OO161_001200"
     CDS             4933..5508
                     /gene="yraP"
                     /locus_tag="OO161_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5462345.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKHIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSKVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5622..6662)
                     /locus_tag="OO161_001201"
     CDS             complement(5622..6662)
                     /locus_tag="OO161_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5462346.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQHYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     QVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQCGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6735..7370)
                     /locus_tag="OO161_001202"
     CDS             complement(6735..7370)
                     /locus_tag="OO161_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5462347.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHILVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7498..8016
                     /gene="yhbO"
                     /locus_tag="OO161_001203"
     CDS             7498..8016
                     /gene="yhbO"
                     /locus_tag="OO161_001203"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5462348.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7996..8439)
                     /locus_tag="OO161_001204"
     CDS             complement(7996..8439)
                     /locus_tag="OO161_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5462349.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8490..8792
                     /gene="yhbQ"
                     /locus_tag="OO161_001205"
     CDS             8490..8792
                     /gene="yhbQ"
                     /locus_tag="OO161_001205"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5462350.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8779..9282)
                     /locus_tag="OO161_001206"
     CDS             complement(8779..9282)
                     /locus_tag="OO161_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5462351.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9276..9800)
                     /locus_tag="OO161_001207"
     CDS             complement(9276..9800)
                     /locus_tag="OO161_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5462352.1"
                     /translation="MLDKLCSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10009..10989
                     /locus_tag="OO161_001208"
     CDS             10009..10989
                     /locus_tag="OO161_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5462353.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10998..11876
                     /locus_tag="OO161_001209"
     CDS             10998..11876
                     /locus_tag="OO161_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5462354.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            12082..13089
                     /locus_tag="OO161_001210"
     CDS             12082..13089
                     /locus_tag="OO161_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR5462355.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13207..14451)
                     /gene="mtr"
                     /locus_tag="OO161_001211"
     CDS             complement(13207..14451)
                     /gene="mtr"
                     /locus_tag="OO161_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5462356.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14605..16494)
                     /gene="deaD"
                     /locus_tag="OO161_001212"
     CDS             complement(14605..16494)
                     /gene="deaD"
                     /locus_tag="OO161_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5462357.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16487..16567)
                     /gene="yrbN"
                     /locus_tag="OO161_001213"
     CDS             complement(16487..16567)
                     /gene="yrbN"
                     /locus_tag="OO161_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5462358.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16674..17558)
                     /gene="nlpI"
                     /locus_tag="OO161_001214"
     CDS             complement(16674..17558)
                     /gene="nlpI"
                     /locus_tag="OO161_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5462359.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17667..19802)
                     /gene="pnp"
                     /locus_tag="OO161_001215"
     CDS             complement(17667..19802)
                     /gene="pnp"
                     /locus_tag="OO161_001215"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5462360.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20049..20318)
                     /gene="rpsO"
                     /locus_tag="OO161_001216"
     CDS             complement(20049..20318)
                     /gene="rpsO"
                     /locus_tag="OO161_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5462361.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20467..21411)
                     /gene="truB"
                     /locus_tag="OO161_001217"
     CDS             complement(20467..21411)
                     /gene="truB"
                     /locus_tag="OO161_001217"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5462362.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMLMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21411..21812)
                     /gene="rbfA"
                     /locus_tag="OO161_001218"
     CDS             complement(21411..21812)
                     /gene="rbfA"
                     /locus_tag="OO161_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5462363.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21976..24648)
                     /gene="infB"
                     /locus_tag="OO161_001219"
     CDS             complement(21976..24648)
                     /gene="infB"
                     /locus_tag="OO161_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5462364.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24673..26160)
                     /gene="nusA"
                     /locus_tag="OO161_001220"
     CDS             complement(24673..26160)
                     /gene="nusA"
                     /locus_tag="OO161_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5462365.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(26188..26640)
                     /gene="rimP"
                     /locus_tag="OO161_001221"
     CDS             complement(26188..26640)
                     /gene="rimP"
                     /locus_tag="OO161_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5462366.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEVVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26847..26923)
                     /locus_tag="OO161_001222"
     tRNA            complement(26847..26923)
                     /locus_tag="OO161_001222"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26887..26889),aa:Met,seq:cat)
     gene            27271..28614
                     /gene="argG"
                     /locus_tag="OO161_001223"
     CDS             27271..28614
                     /gene="argG"
                     /locus_tag="OO161_001223"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5462367.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
BASE COUNT         6828 a         7885 c         7197 g         6726 t
ORIGIN      
        1 ggaaaatcga tactggaaaa actctaactc ggtggccagt ttttgttgac cacttcaaac
       61 tggcaatacg cctgttcctg ctctgcgggt aaggcagtta aacttgtcta tatggtcagc
      121 cccgtactta ccaccactgg acatcagaca ggatattaca aactcaatga cagcctggat
      181 attaaaacca cattacaggc aaacgacatt ccaggactca caaccgacca ggttgtctct
      241 gttaacaccc gattcacaca gataaaaagc agcaccgtat attctgctgc aacccaaacg
      301 ggtgtttgcc agggtgacac gtctcgttat ggacccgtta atattggtgc aaataccacc
      361 tttaccctgt atgtcaccaa gccatttctc ggctcgatga ccattccgaa aacggatatt
      421 gccgtcatta aaggcgcgtg ggtcgatgga atgggaagcc cgtctacagg tgacttccat
      481 gatttagtca agttatcgat tcagggaaat ttcaccgccc cacagtcgtg caaaattaat
      541 cagggcgatg ttattaaggt taattttgga ttcatcaatg gtcagaagtt taccacccgc
      601 aatgccatgc cagacggttt tactccagta gactttgata tcacttatga ctgtggtgat
      661 acttcaaaga ttaaaaactc gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac
      721 cagtacaacc tggtcgccag gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt
      781 attgaaaatc tcggcggcgg agttgcaaat attccttttc agaacggtat ccttcccgtt
      841 gatccttccg ggcatggcac cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt
      901 gagctggaaa caggaaaatt tcagggcaca gccaccatta ccgtcatggt gcggtaaaag
      961 aagacgttgc gttttgatta acgcaacgct tcggcactgt tacccctgct gctccagcgc
     1021 atacttatac agcgcatttt tcttcacgcc atgaatttct gcgaccagcg ccgccgcttt
     1081 tttcagcggc agttctgcct gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc
     1141 ttcttcctgt gctttatgac cttcgacaat cagcaccatt tcgcctttgc gacggttttc
     1201 atcttccttt acccacgcca gcagttcgcc aacgggcgcg ccgtgaatgg tttcccaggt
     1261 tttggtcagc tcacgcgcca gaaccacgta gcgggattcg cctaataccg caacgatatc
     1321 ttccaggcta tctaacagac ggtgggtgga ttcataaaaa atcagcgtgc gcggctccgc
     1381 ttcaatggct tttagcgcat cacggcggcc ttttgatttg gcaggtaaaa agccttcgta
     1441 acagaaacgg tcagagggta aacccgcggc gcttaacgca gtgatagcag cacacggccc
     1501 gggtagcggc accacgcgaa tccccgcttc acggcaggta cgcaccagat ggtagccagg
     1561 atcgttaatt agcggcgttc cggcatcgga aaccagcgca atgttttgcc cctcttgcag
     1621 cttcgccagc agcgtttcgg ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg
     1681 ggcattaatc ccaaaatgtt gcagcaataa accggtgtga cgagtatcct cggcggcaat
     1741 cagatcaacg gcctgtaata cctctaacgc acgctgggtg atatccgcca gattgccgat
     1801 tggtgtcggt acaatgtaaa gctggccctg agaattatcc gccgattggt gttgtttcat
     1861 tgtgtcgtcc gtattgccga tttaatattg agcattgcgt aaaaaaatat cactggatac
     1921 attatggtac cctcaacatt ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg
     1981 gcagccctga ttttcgccgg ttgtggcacc catactcccg ctcagtccac tgcttatatg
     2041 cagggcacgg cgcaggctga ttctgccttt tatcttcagc agatgcagca aagctctgat
     2101 gataccagga tcaactggca attactcgcc attcgtgcac tggtgaaaga aggtaaaacc
     2161 gggcaggcgg ttgagttgtt taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc
     2221 gagaaaacac tgctggcggt agagattaaa ctggcgcaga aagattttgc tggcgcgcaa
     2281 aacttgctgg cgaaaatcac acctgccgat ttagaacaaa accagcaagc gcgttactgg
     2341 caggcaaaaa tcgatgccag ccaggggcgt ccttccattg atttactgcg cgcgttaatt
     2401 gctcaggaac cgctgctcgg cgcgaaagaa aaaaagcaga atattgatgc cacctggcag
     2461 gcgctctcct ccatgactca ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat
     2521 attctgcaag gctggctgga tctgcagcgc gtctggtttg ataaccgtaa cgatcccgat
     2581 atgatgaaag ccgggatcgc cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa
     2641 atgctgccaa cgcagttggt taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc
     2701 gccctgctgt tgccgctgaa tggccaggca gcggtatttg gtcgcactat tcagcaaggc
     2761 tttgaagcgg cgaaaaatat cggcactcag ccagtggcgg ctcaggtagc tgccgcacct
     2821 gccgcagacg tagcagaaca acctcagccg caaactgcgg atggcgttgc cagcccggca
     2881 caagcctcgg ttagcgatct gaccggtgat cagcctgcgg cccagccggt gcctgtaagc
     2941 gccccggcga caagcaccgc agcggtaagc gcacccgcaa atccatccgc agagctgaaa
     3001 atctacgata cctcatcaca accacttagc cagatcttaa gccatgttca gcaggatggc
     3061 gcgagtattg tggttggtcc gttgctgaaa aataacgttg aagagttgct taagagcaac
     3121 accccgctga acgtgctggc actgaaccag ccggagaata tcgaaaaccg cgtcaatatt
     3181 tgttacttcg cgctttcacc ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac
     3241 cagggtaaac aagcgccgct ggtgctgatc ccacgcagtt cattgggcga tcgcgtagcc
     3301 aatgcgtttg cgcaggagtg gcagaaactg ggcggcggca ccgttctgca acaaaaattt
     3361 ggttccacca gcgaattacg cgcgggtgtt aacggcggtt ctggtatcgc tttaacgggt
     3421 accccgatta ctcccagagc gacaaccgac tccggcatga cgaccaacaa tccaacgctg
     3481 caaaccacgc caaccgatga ccagttcacc aataatggcg gtcgtgtcga tgcggtgtac
     3541 attgtggcaa cgccgggtga aatcgctttt atcaaaccga tgatcgccat gcgtaacggt
     3601 agccagagcg gtgcaatgct gtacgccagc tcccgcagcg cacaaggcac cgcaggcccg
     3661 gatttccgtc tggagatgga aggtttgcag tacagcgaaa tcccgatgct ggcgggcggt
     3721 aatctgccgt taatgcagca ggcactcagc gcggtgaata acgattattc actggctcgc
     3781 atgtatgcga tgggcgtcga tgcctggtcg ctggcaaatc atttctcaca aatgcgccag
     3841 gttcagggtt ttgaaatcaa cggtaatacc ggaagcctga cggcaaaccc ggattgcgtg
     3901 attaacagga agttatcatg gctacagtac caacaaggtc aggtagtccc cgccagttaa
     3961 ccaccaaaca gaccggcgat gcgtgggaag tacaagcgcg tcgctggctg gaaggcaaag
     4021 gactgcgatt tgtcgccgct aacgtgaacg agcgtggcgg tgagatcgat ctgataatgc
     4081 gtgaaggctg gaccaccgtt tttgtcgagg tgcgttaccg ccgctctgcg ctttatggcg
     4141 gcgcggcagc cagtgtgacc cgcagcaaac aacacaaatt attacagact gcccgcttgt
     4201 ggctcgcgcg tcataatggg agttttgata ctgtggattg ccggttcgat gtggtagcct
     4261 tcaccgggaa tgaggttgag tggattaagg atgcctttaa tgaccactca taattaaggt
     4321 ttaaggatta gcgtgcaaga aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa
     4381 attgcggcgg cagaggcgct tccggatgcc atctcccgtg cagccatgac gctggttcag
     4441 tctctgctca atggcaacaa aatcctctgt tgtggtaatg gaacttccgc tgccaatgca
     4501 cagcattttg ctgccagcat gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc
     4561 attgcactaa atactgataa tgttgtctta acggcgattg ccaacgatcg cttacatgat
     4621 gaagtgtatg caaaacaggt gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt
     4681 tccacccgtg gcaacagccg cgatattgtt aaagcagttg aagccgccgt tacgcgtgat
     4741 atgaccattg tggcattgac cggctatgac ggcggcgaac ttgcaggttt gttagggcca
     4801 caggatgtgg agatccgcat tccttcgcat cgtagtgctc gcattcagga aatgcatatg
     4861 ctgacggtaa attgcctgtg cgatctgatc gataacacgc ttttccctca ccaggatgat
     4921 taaggagaat acatgaaggc attatcgcca atcgcagtcc ttatttccgc gctgctgttg
     4981 caaggttgtg ttgccgctgc cgtagtgggt accgctgctg tgggtaccaa agccgcaact
     5041 gacccacgca gtgtcggcac ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc
     5101 gcattgtcga aagacgaaca gattaagaaa gaaacccgta ttaatgtcac ggcgtatcag
     5161 ggcaaagtgc tgctggttgg gcagtcacca aatgctgaac tttcggctcg cgccaaacat
     5221 attgctatgg gcgtagacgg tgccaacgaa gtgtataacg agattcgtca gggccagccg
     5281 attggtctgg gcgaagcatc taacgatacg tggatcacca ccaaagtgcg ttcgcagctc
     5341 ttaaccagcg acctggtgaa atcgtccaaa gtgaaagtga ccaccgaaaa cggtgaagtg
     5401 ttcctgatgg ggctggtgac tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg
     5461 gtgagcggcg taaagcgggt aaccacagcg tttacgttta ttaaatagca gccccttgta
     5521 atgcctgatg cgacgcttgc cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg
     5581 gataaggcgt ttacgccgca tccgacatgt tttccctcaa atcagaacaa cagcgccagc
     5641 ccgccgacaa tcacaccaga cactgccacc atcgcccctg tcagccataa ggctttcgcc
     5701 gggaacgctt tgcgcagcat aatcagtgac ggcaaactca ctgccgggag cgtcatcaac
     5761 aatgccagcg ccggagcggt tcccatacct gccagcatca tcgtttgtac aatcggaatt
     5821 tctgctgccg tgggaatgac aaacaagcat cctgctaccg ccatcgccac cacccacatc
     5881 aggctgttat cgacagcacc atcggcatgg gggaataacc agacgcgtgc ggcacccaac
     5941 accagtactg caaggatgta aaccgggatc gtactccaga aaagtgtcca tagcgccctg
     6001 ccccagcggc taaaaaaacc gcactgtgct tccggtatgt caatttcgac cggtgcctgc
     6061 gtttgcggtg tttcacgcac ccatttttgc accagcgtcg caatcagcaa caccatcacc
     6121 agcccggcca ccagacgaat cgccgcaaaa ccccagccga ggacaaaccc cataaacacc
     6181 tgcgtcgccg ggtttaacac cggattgccc atccagaatg ccagcgcacc gcccatcgat
     6241 acctgttggc gacgcatccc cgccgcgacc ggagccgcac agcaggtaca catcatgccc
     6301 ggcagcgaaa acagcgttcc cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc
     6361 aacaaccagt cacgcgggat cagcacctga atcaacgaac cgaggatcac ccccagcacc
     6421 gccgctttcc atactgcgag gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga
     6481 ttagcatccg cctgcgcaag gatagattta ccgatactgt gggtttcggc tgcagtaaac
     6541 gctttgccgt agtaatgttg ccatttcaca taccagagac cggcaatgac aacgagaaag
     6601 aaaagcgcgg gtttccacca ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc
     6661 atagcattcc ctaggaataa gttatgataa ttgagcgcgt gaatattacg ctcactatca
     6721 attcttggga ataattattc agctcttttg cgtaattctg aagagcttaa aatcgtcacg
     6781 ccttcatgct caggccgcat cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca
     6841 atggatttcc agttacctgg taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc
     6901 cgctgtttgc tacgatcgcc cagtaacatc gacgggcgag caatggtcaa tttcggccag
     6961 ttctgggcaa ttaatgcttc ttccatctcc cctttgacgc ggttatagaa aaacggcgag
     7021 tgggcattgg cccccatagc actgaccacc agaatatgct gcgcgcccag ccgccgcccg
     7081 gttaatgcgg tatctactac cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc
     7141 gcttctcgcc gcgtggtgcc gagacaacaa aacacaatgt cgatgggttc ggtgacctgc
     7201 gccaacgcgt cactcagttg cggatcatgg ggattaaaca ccccgggcat atcgcccaac
     7261 ggacgtcgcg tcggcgcagc aatggcgtta actttcggtt cattaatcaa catccgcagc
     7321 aggtgaccgc ccaccagccc cgttgcgcct gtaatcagta cctgactcat cttcgctcct
     7381 ttacagaatt gtctgccttg cgctccacgg ctcatgcacc aggcttaata gaccatgagg
     7441 taattatccc cgattgtggg aaattcgcct catccaatgc aacaacgcgg aggaagcatg
     7501 agtaagaaaa ttgccgtttt aatcagtgat gaatttgagg attcagaatt tacttcaccc
     7561 gcagacgagt tccgtaaagc cggacacgaa gtaattacca ttgaaaagca agcgggtaaa
     7621 acggtgaaag gcaaaaaagg agaagccagc gtgaccatcg ataaatccat cgatgaagtg
     7681 acgccagcgg agtttgatgc cctgctgcta ccgggcggcc attcaccgga ttatctgcgt
     7741 ggggacaacc gttttgtcac ctttacccgt gattttgtga atagcggcaa acctgttttc
     7801 gccatctgtc acggtccgca gctgctgatc agcgccgatg tgattcgggg gcgcaaactg
     7861 accgcagtta aaccgatcat tattgatgtt aaaaatgcgg gcgcggaatt ttacgatcag
     7921 gaagtcgtgg ttgataaaga tcagctggtt accagccgga caccggacga tctgccagcg
     7981 tttaaccgcg aggcgttacg cctgctcggt gcctgagtcg cgtagccaaa tcattttttt
     8041 accaaagccc agcgtgttgt cggtgaattt gatttcatcg agccggattt cccacaccgg
     8101 ggccgacagc attctggcaa ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct
     8161 ttcctcacct tccagcctgc ggatctcacc tttaaactgc acaccgcgaa ttaacgctac
     8221 cgttttcggc tgaccgttta ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc
     8281 gtggcgcgtt ttttcttccg tcaaaatgta gaaggcgact ttctgcgcat caaacagata
     8341 aaaagcatta gcgcaccaca gttccccttc ctgctgcaca caccaggtga caacatgttg
     8401 ttttgccaac caacggctga tggtgatgag tgtttccatt gctgttctct tttatactgt
     8461 gggcctgacc ttaacatgcg acgcagacga tgacaccctg gtttctttac ttgatccgta
     8521 ccgccgacaa taagctttat accgggatca ccacggatgt cgaacgccgc tatcagcagc
     8581 accaaagcgg caaaggggcg aaagcactgc gcgggaaagg agaactgacg ctggcgtttt
     8641 ctgcgccagt gggcgaccgt tcgctggcac tacgggcgga atatcgcgtt aagcaactga
     8701 ccaaacggca aaaagagcgt ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca
     8761 gcctgcaaac cccggagatt aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt
     8821 tacgccattc agcgcgtcat ccgctaaacg atgtacctgg aaggcgcttt cagtgcccgg
     8881 ccagcggcaa cgcagatcgt gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc
     8941 cggatcgccc agcgtcacca ctgcggcata gccgaactca ttaagcgaat cgagtccttc
     9001 atagaccagt tggcgtgcca gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc
     9061 catgccgacc cattgcaggt cttcgccctg cacatcaacc ggactaaatg ccacatagcc
     9121 aatgacctga ccttcgtcat ctgtcgccac cagccccagc gtcagaaagc catcttcacg
     9181 cagatcgtga accagcttcg cttccgcatc actttcgaat gagcgacgca gcagggcatc
     9241 aatacccggc gcatcaatgg gaatttctac tcgaattagc atggttcacc taccgatgtc
     9301 tgtttggttt caggcgcggt tttcattccc gcctcaacaa aatccgccag ttgcagcagc
     9361 atcatgcgta aggctttcgg catttgctcc agttcaatgg cgtccatcag gtttttcaca
     9421 tacagcccca gctccgtatc gccttcaatc accagccgac gctggaagaa gagcgtatct
     9481 ggatcttgtt tacgcgccgc aatcatcagc agatcgctgg cgtcggcact aaaactcaca
     9541 tcagcttgcg cgttctggct gaccaccagt ttgccattca ccaccgaggt aaaccattgc
     9601 aggtcaatat cacgcacatg aatacttaac cagcggcctt caagaaactc cagctcgcca
     9661 tcatccagcg cctgacggaa ttgccagctt aagacctgct caagaacctg gcgttttagc
     9721 gcaaatggcg tcagttttac cggtacactc aacagagatg gccccaaatg cacaatacgg
     9781 gaacacagtt tatccaacac gagttttact ccctgtttca acaatcatcc tattttgcca
     9841 tatcagaaaa ataacatagc ggtataaatc aacaattcca tatgaaattg ctgctaccac
     9901 caatacaact ttaactgcct taaatcaaaa attgtcgcag caaggttaac taaaatccca
     9961 gttcgttaac atttttgcgt tttgatagcg caaccttcag gaaaaattat ggagctgctc
    10021 tgccctgccg gaaatctccc ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt
    10081 tatatcgggc taaaagatga taccaatgcc cgtcacttcg ccggccttaa ctttaccgag
    10141 aaaaaattgc aggaagcggt gagttttgtc catcaacatc gccgcaaact tcacatcgcg
    10201 attaacactt ttgcgcatcc ggacggttac gcccgttggc agcgcgccgt ggatatggcg
    10261 gcgcagctgg gtgccgacgc gctgatcctc gccgacctcg ccatgctgga atacgccgcc
    10321 gagcgttatc cgaatattga gcgccacgta tcggtgcagg cttcggcgac caatgaagag
    10381 gcgattaact tttatcatcg ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg
    10441 tcgattcatc aggtgaaaca gctggcacgg gtcacacctg taccactgga agtgtttgct
    10501 ttcggcagcc tgtgcattat gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt
    10561 gagtcgccta acaccgtggg cgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg
    10621 ccgcaggggc tggaatcccg cctgaacgaa gtgctgatcg accgttatca ggacggcgaa
    10681 aacgcaggtt atccgacgct gtgtaaaggg cgttatctgg tggacggcga gcgctatcac
    10741 gcgctggaag aaccaaccag tctcaatacc ctggaactgc tgccggagtt aatggcggcg
    10801 aatattgctt cggtgaaaat tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg
    10861 gcgaaagtct ggcgtcaggc tatcgaccgt tgtaaggccg atccgcaaag cgcgtggatg
    10921 gagacgctcg ggtcgatgtc cgaaggcacg cagaccaccc ttggcgcgta tcaccgtaaa
    10981 tggcagtgag aagagcaatg aaatattcct tagggccagt gctgtggtac tggccaaaag
    11041 agacgctgga agatttttat cagcaggccg ccaccagcag cgccgacgtg atttatcttg
    11101 gtgaagcggt atgcagcaag cgccgggcaa ccaaagttgg cgactggctg gagatggcaa
    11161 aatcgctcgc cggaagtggc aagcagattg tgctctccac gctggcgctg gtgcaggcat
    11221 cctctgaact gggcgaactg aaacgctatg ttgagaacgg tgagtttctg attgaagcca
    11281 gcgatctcgg cgtggtgaat atgtgcgctg aacgcaaact gccgttcgtc gccgggcacg
    11341 cgctgaactg ctacaacgcg gtgacgctga aaatattgct caaacagggc atgatgcgct
    11401 ggtgtatgcc ggtggagctt tcccgcgact ggctggtgaa tctgcttaat cagtgcgatg
    11461 agctgggcat tcgcaaccag tttgaagtgg aagtcctgag ctacggtcat ctaccgctgg
    11521 cctactccgc tcgctgcttt accgcgcgtt cggaagaccg cccgaaagat gagtgtgaaa
    11581 cctgctgcat taagtatccg aacgggcgca acgtgctgtc gcaggaaaac caacaagtgt
    11641 ttgtactcaa tggcattcag accatgagcg gctacgttta caacctcggt aacgagctga
    11701 catccatgca gggattggtt gatgtggtcc gcctgtcacc gcagggtact gacactttcg
    11761 cgatgctcga cgccttccgc gctaatgaaa atggcgctgc gccactattg ttgacggcaa
    11821 acagcgactg taacggctac tggcggcggc tggcaggact ggagctgcaa gcctaagtaa
    11881 atatgctcgt catacttcaa gttgcatgtg ctgcatctgc attcgttcac cccagtcact
    11941 tacttatgta agctcctggg gattcactct cttgtcgcct tcctgcaact cgaattattt
    12001 agagcacagc tcactttgtt aacaacttta actactcttt aatgcagtat taaagattaa
    12061 tcggtaacaa ggtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg
    12121 gcccccattc ccgaaggttc ttcagcgcga gaagcattct cccactctct cgatctcgcc
    12181 cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgact
    12241 ggcattgcca gtgctgccac gtcggtattg attggctatc tggcggcgaa taccaccacg
    12301 ctgcatctgg ggtctggcgg cgtgatgttg cctaaccact caccgttggt cattgccgaa
    12361 cagttcggca cgctcaatac actctatccg gggcgaatcg atttggggct gggtcgtgcg
    12421 ccaggtagtg accaacggac aatgatggcg ctgcgtcgcc atatgagcgg cgatattgat
    12481 aatttccccc gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg
    12541 catgtgcgcc cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagt
    12601 ctttacagcg cgcaactggc ggcgcagctt ggtctgccgt tcgcgtttgc ctcacacttc
    12661 gcgccggata tgttgttcca ggcgctgcat ctttatcgca gcaacttcaa accgtcggca
    12721 cgactggaaa aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc
    12781 gatgccgaat tcctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgcggcgaa
    12841 accgggcaac tgccgccacc gattcaaaat atggatcagt tctggtcgcc gtccgaacag
    12901 tatggtgtgc agcaggcgct gagtatgtcg ttggtaggtg ataaagcgaa agtgcgtcat
    12961 ggcttgcagt cgatcctgcg cgaaaccgac gccgatgaga ttatggtcaa cgggcagatt
    13021 ttcgaccacc aggcgcggct gcattcgttt gagctggcga tggatgttaa ggaagagttg
    13081 ttgggatagt gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta
    13141 gacctgataa gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac
    13201 cgctgattac tgatacaccg gcagtaaatt aaagctcgat aaaatatgta ccaatgcgtt
    13261 gccgacgcca aacaccagaa tcagcgtaat catcggcttg ccgccccaga cgcggaattt
    13321 cgggctgcca aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca
    13381 gatggtagcc gctaaaccag cataaccaat ggcgtacagg aatccattcg ggaacagcag
    13441 accacccaca acaggcggtg caaaggtcaa caatgctgtt ttcaagcggc ccatagccga
    13501 gtcatcgaaa ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa
    13561 cgaactcgct accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt
    13621 cagcacgccg cttaatgcct gtaccagcac atcaatatta ccgcccttct ctgcaatacc
    13681 gataaactcc ggacgcggga tgttacccat cgtcgccagc aaccagatgg tatacagcgc
    13741 cagtgccatc agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta
    13801 atacttcatc aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcaa
    13861 tgtcatcagc agatacggcg cataagatgc attgctttct gcaacgttga acaatgtcgc
    13921 aggctgcaca tgccccaaca ggctgccaaa ggtgaggaag aaggtaatga ctttcgcccc
    13981 cagcacaatc gccgtcatgc ggctgacggc cttagtactc aaccacacca caaacgctac
    14041 cagcagtgca aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa
    14101 ggtgtgatgc agaattgaac cactggcaga aatataggca taggtcagga tatagagcac
    14161 aaaggcaatg gaaataccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat
    14221 ggtgtcaaaa ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga
    14281 atgcagcata cagaaccagg taaagatcag cgccgccatt gaccagaaaa accacgcccc
    14341 ggacatgacc actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac
    14401 cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc
    14461 tctccagtga aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacgcgagt
    14521 acaaaagaca gaaaaaaagc cccgatggta aaaatcgggg ctgtatatat tattttacag
    14581 attgtgttcg ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga
    14641 gtcatcacga cgcggagcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc
    14701 acggccacct tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gttcgccacc
    14761 gaaaccacga ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa
    14821 ctgcatgttc atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc
    14881 cggcatacct ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccaat
    14941 gtaacggctg ctgatgtcgc cttcgttagc aatcgcacca acgatatgac gaacttcaac
    15001 accatcatca cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc
    15061 acgacgcgga cgatcttcac ggtcaccacg cgggccacgg tcgttacgat cgcgcggacc
    15121 acggtcatca cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggtacgat
    15181 cagagtacgt tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag
    15241 ctcttcacct tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc
    15301 gctgctttcc agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag
    15361 cagttctgcg ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt
    15421 gcgcagcaga cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc
    15481 acgaccggta cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt
    15541 aactaccagg ctgatacgct caacgtccag gccacgggct gcaacgtcgg tcgcaatcag
    15601 gatgtccaga cgaccatctt tcaggcgttc cagtgtctgt tcacgcagcg cctggttcat
    15661 gtcaccgttc agcgcggcgc tgttgtagcc gttacgctca agagcttcag ccacttccag
    15721 agtcgcgttt ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa
    15781 acgtaccagt gcttcgtttt tgcgcatacc ccagacagtc cagtagctct ggctgatgtc
    15841 aggacgggta gtcacgctgg actgaatgcg cacttcctgc ggctctttca taaagcggcg
    15901 ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc
    15961 cgggatctgc gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc
    16021 agcttcatcc agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg
    16081 gtccagcaga cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa
    16141 ttgcacgtca taacgctggc cgccgtacag agcaaccaca tttacgccgc gcatgtgttt
    16201 agagaaatcc gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac
    16261 cagaatctgt ggtgctttca gctcaggatc aagattctgc aacagaggta aagagaatgc
    16321 tgcagttttt ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg
    16381 tggaatacac tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc
    16441 aaggatagga gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta
    16501 cgtgtgcctc aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa
    16561 ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc
    16621 acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc
    16681 tggtccgatt ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg
    16741 tatcggtgct caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg
    16801 gcgctgtcca aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg
    16861 agatgctcag cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt
    16921 tgttcgctaa tgttgcccaa gtagaactcg acaatgttcc atccccactg ttccttatcc
    16981 gatttttcga agtgctgttt caacacttct ttagcctgct tctcatcgag cttctgctcg
    17041 gcgagataaa gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc
    17101 agatcatctt gcgctaactt atcacgaccg ccgtaatata atgcgatccc gcgattcaag
    17161 tgcgcgtagt tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca
    17221 tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat tgaacacttc aggcatatcc
    17281 ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta
    17341 tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga
    17401 ctggcaagga tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat
    17461 ggtaccgcga ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt
    17521 gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg
    17581 ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc
    17641 gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt
    17701 gctgcagcag gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg
    17761 cgatcaactt ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact
    17821 ttctcaacgc gtttgtcagc gatttgagag atgtggacca gaccttcttt accgccgccg
    17881 atggcaacaa atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc
    17941 acttcgattt ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg
    18001 tcggtcgctg cgatcttcac agtaccgtca tcttcgattt cgatggtagt gccagtttct
    18061 tcggtcaggg cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg
    18121 ttgatcttga tggtatggat acgcggtgcg aactcagaga tatcgccacg cggcgcgttg
    18181 atcgcctgtt ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca
    18241 acctgcatga tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata
    18301 ccctcgcggg aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa
    18361 atgtcagaca gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca
    18421 acggcagctt tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa
    18481 gccatagaag aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg
    18541 aatttgtcca tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca
    18601 atttcacgac gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag
    18661 ttgtagtgga acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg
    18721 tcacgagcag tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc
    18781 gcagaaccgt gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct
    18841 ttttcacgac cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc
    18901 tcgatagcgt gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc
    18961 agcgtcgcga tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg
    19021 gtgatgcggt aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc
    19081 gcttcgttta ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt
    19141 tcattgatgt tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc
    19201 atctggtctt cgctcagcag ttcagcttca gattcaacca tcagaacagc ggcttcagta
    19261 ccggcaacaa ccagatccag tttgctctct ttcagctcgt cctgagtcgg gttcagtacg
    19321 tactggtcat tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca
    19381 gacagagaca gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg
    19441 ttaacagaaa ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc
    19501 gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tggacgacct
    19561 tcacgacgga agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta
    19621 acggtcagtg ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca
    19681 aataccgcgg tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg
    19741 ccggtttcca gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc
    19801 aatgtaatat cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat
    19861 cttctgcgca tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc
    19921 tcattagccg cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc
    19981 cagtgaattg ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact
    20041 cgcaggaatt agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct
    20101 ttacgtttca ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga
    20161 cggctgtggt gatctttttt gtgctctgca aagtgcccct gcaggtggtt gatctgtgca
    20221 gtcagcagtg ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg accaaactca
    20281 gaaacgattt tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag
    20341 aatgaaagga cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa
    20401 caatttacgc gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg
    20461 ttaacattac gccgggtatt caaccaccag gcgacgaggc gcgacgcggc cttcatcgtc
    20521 aatttcgccc ataccgataa acttgccgtt ctcaccttct gtaacgcgaa ccagtccttc
    20581 cagcggcgca ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa
    20641 cggaagattc accaccgggt agtccgaagc tggactgtcc attagcatca gtaatggatc
    20701 aagtaactcc gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg
    20761 ttccagggtc accatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat
    20821 aacatgcgcg ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt
    20881 gccttttgag cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa
    20941 ttcataaacg gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc
    21001 gtacagtttt ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc
    21061 gccacggaaa gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc
    21121 ttcaacgatt tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac
    21181 ccgatagcgt ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaagca
    21241 aattggcaac atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc
    21301 gttataaatc cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc
    21361 cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc
    21421 ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg
    21481 tttgacatgc gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg
    21541 atacgcaggc gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac
    21601 gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact
    21661 ttggcatacg ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg
    21721 cgaggatctt taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct
    21781 acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct
    21841 gttagcccct ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga
    21901 cgcgccagcg tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat
    21961 ggaaccctaa aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat
    22021 cacatcgcca gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt
    22081 acggacttcg ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat
    22141 aaccacgttg tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac
    22201 catacagcct gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag
    22261 accgataatc tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc
    22321 gtcaatcagg ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac
    22381 tttacgtgca gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc
    22441 agccagggtg gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt
    22501 aacttcgtca gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac
    22561 gtctgccttc aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat
    22621 gttctcgagt ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg
    22681 atacagtgca acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc
    22741 agccggtacg ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac
    22801 ttcctgacct agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac
    22861 gatatcgccc ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc
    22921 tttatcgagg aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag
    22981 ctccagaact tccgcctgca gcaggatagc gtccagcagt tcatcgatgc cggtacccgc
    23041 tttcgcagat acgtgtacga actggctttc accgccccac tcttctggca ggatgccata
    23101 ctgggagagt tcgttcttaa cacgatccgg atcggcttct ggtttatcaa tcttgttcac
    23161 tgcaaccacc accggtacct gcgccgcttt cgcgtgctgg attgcttcga tggtctgcgg
    23221 catcacgccg tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg
    23281 agcacgcatt gaagtaaacg cggcgtgccc tggggtgtcc aggaaggtga tcatgccgtt
    23341 ttcagtttca acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc
    23401 cactttcgtt gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat
    23461 gatggtcaca accggcgcgc gcggttcagc cgcagccccc gtgtcacggt cgctcattac
    23521 cgcctcttcc agctcgtttt cacgacgcag gataactttg tggcccatct cttcagcaac
    23581 cagctgtgcg gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat
    23641 catcgctttg atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt
    23701 gatggtttcg ccgatcacaa cgtcacggtt aacggcctga gctggcttct ggaagccttg
    23761 ctgcagggaa gaacctttac gttttccgcc tttacctcca cgtactgctg cgcgagcttc
    23821 ttcacgatca gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc
    23881 gttacgacca cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg
    23941 agcatgttga gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc
    24001 agtccatttg ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc
    24061 ttcgagttta cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc
    24121 acggcgggct ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt
    24181 gtctttttcc gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc
    24241 ggcctcacgt tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg
    24301 ggcttgctct tccgcttcac gctgcgcttg ctcttccgct gcaaggcgtt cagcctcttg
    24361 cggatcgcgt ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg atttgctttt
    24421 tccaccggta ccaggaatgt taagggtgct gcgtgtttta cgttgcagcg tcaatttgtc
    24481 cgggcctgaa tttttctgat tcaggtggtc aatcaaagtc tgtttctctt gtgcggacac
    24541 agagtcgtca gcagacttcc ggatacctgc atcagcaaat tgctgtacca ggcgttccac
    24601 ggaggtctgt cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc tgttccttcc
    24661 tgctacagtt tattacgctt cgtcaccgaa ccagcaaata ttacgggcag ccataatcag
    24721 tgctccggct ttttcgtcgg tcaccccttc gatatcagcc agatcatcaa tgccctgttc
    24781 ggcgagatct tccagcgtac aaacgccacg ggcggccagt ttgaatgcca aatcacgatc
    24841 tatcccttca aggttcagca gatcgtcagc cggtttgtta tcaccgaggc tttcttcctg
    24901 ggcctgtgca atggtggcca gtgcattttt agcacgctcg cgcagtgctt caacggtcgg
    24961 ctcatcaagg ccttcgattt ccaacagctc tttcatcggc acataggcca gttcttccag
    25021 cgtcgagaag ccttcttcta ccagaacagt cgcgaagtct tcgtcgatgt cgagatattt
    25081 ggtgaaggtg tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca ggtcgtcaac
    25141 ggtcatcacg ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt tctgaccgtt
    25201 acggccaatc gcctgcgcca ggttaccggc ttcaacggcg atatccatgg tgtgtttatc
    25261 ttcatccacc acgatagaag caacgtctgc cggtgccatt gcgttaatca caaactgcgc
    25321 cgggttatca tcccacagga cgatatcgat acgctcgccg cccagttcag tagaaaccgc
    25381 ctgaacacgc gcgccacgca tacctacaca ggcacctacc ggatcgatac gtttatcgtt
    25441 ggttttcacc gcgattttcg cacgagaacc cggatcgcga gccgctgctt taatttcaat
    25501 cacttcttcg ccgatttctg gcacttcaat acggaacagt tcgatcagca tttccggctt
    25561 ggaacgagtg acgaacagtt gcgcgccacg cgcttccggg cgaacggaat agagcacgcc
    25621 acgaacgcgg tcgccagggc ggaagttttc acgcggcagc atatcttcgc gcaggatcac
    25681 ggcttcagca ttgttgccca gctccagaga gatgttgtcg cggtttactt ttttcaccac
    25741 gccggtgatg atttcacctt cgtgttcacg gaactgatca accaccatcg cacgttcggc
    25801 ttcacgcact ttctgcacga taacctgttt tgccgtctgg gtagtgatac ggtcaaaggt
    25861 aacagactca atctgatctt caacgtaatc gcccaggttc aggctttcat cttcataacg
    25921 tgcggcttca agggtgattt ccttggtcgg ctgggtgact tcatcaacaa ctaaccagcg
    25981 acggaaggtg tcaaaatcac cgcttttgcg atcgatctgt acgcggacgt cgatctcttg
    26041 ttcatatttt ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga aaatcttctc
    26101 gcgaggtagc gccttttcat tggatacggc ttcaactaca gccaaaattt ctttgttcat
    26161 cgcgggcttt tcacctcatc cagactatta aaagtgggga accaggttcg ccttctggat
    26221 attactcagc gcgaacactt catctttacc ttcgacggta actgtgatca tttcaccgtc
    26281 taccgctttg ataacgccct gccatttacg acggttttgt accgccatac ggagaaccag
    26341 agtcaccact tctccgacaa aacgggcgta gtgttcagcc gtgaacagtg ggcgatcgag
    26401 acccggtgag gagacttcca ggttataagc aacggtgatg ggatcttcaa catccagcac
    26461 agcacttacc tggtggctca catcagcaca atcatcaaca ttgatgccat cttcactatc
    26521 aatatagatg cgcagtgtgg atgtgcgacc gcgaataaat tcgatgccaa ccagttcaaa
    26581 acccagggcc tcaactggcg cagtaatcat ctctgttaat ttttgctcta atgtggacaa
    26641 gcccaccccc aagacataaa aaaagggcct aaagcccagt tattctgtag tcagataaca
    26701 aaaaaccccg ataaatcggg gctttatata actgaaccct ataaccgcaa ctgcggtctg
    26761 gagcactttc cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc ttcacagtat
    26821 atttgaaaaa ggactctaag ggaaagtggt tgcgggggcc ggatttgaac cgacgacctt
    26881 cgggttatga gcccgacgag ctaccaggct gctccacccc gcgcctgaaa cgcggcaaat
    26941 tctactcgtt ttgggtaaaa gatgcaaata ctgctgggat ttggtgtacc gagacgggac
    27001 gtaaaatctg caggcattat agtgatccat gccacatttt gtcaacgttt attgctaatc
    27061 acgtgaatga atatccagtt cactttcatt tgttgaatac ttttgccttc tcctgctctc
    27121 ccttaagcgc attattttac aaaaaacaca ctaaactctt cctgtctccg ataaaagatg
    27181 attaaatgaa aactcattta ttttgcataa aaattcagtg agagcggaaa tccaggctca
    27241 tcatcagtta attaagcagg gtgttatttt atgacgacga ttctcaagca tctcccggta
    27301 ggtcaacgta ttggtatcgc tttttctggc ggtctggaca ccagtgccgc actgctgtgg
    27361 atgcgacaaa agggagcggt tccttatgca tatactgcaa acctaggcca gccagacgaa
    27421 gaggattatg atgcgatccc tcgtcgtgcc atggaatacg gcgcggagaa cgcacgtctg
    27481 atcgactgcc gcaaacaact ggtggccgaa ggtattgccg ctattcagtg tggcgcattt
    27541 cataacacca ccggcggcct gacctatttc aacacgacgc cgctgggccg cgccgtgact
    27601 ggtaccatgc tggttgctgc gatgaaagaa gatggcgtga atatctgggg tgacggtagc
    27661 acctataaag gaaacgatat cgaacgtttc tatcgttatg gtctgctgac caatgctgaa
    27721 ctgcagattt acaaaccgtg gcttgatact gactttattg atgaactggg cggccgtcat
    27781 gagatgtctg aatttatgat tgcctgcggt ttcgactaca aaatgtctgt cgaaaaagcc
    27841 tactccacag actccaacat gcttggtgca acgcatgaag cgaaggatct ggaatacctc
    27901 aactccagcg tcaaaatcgt caacccgatt atgggcgtga aattctggga tgagagcgtg
    27961 aagatcccgg cagaagaagt cacagtacgc tttgagcaag gtcatccggt ggcgctgaac
    28021 ggtaaaacct ttagcaacga cgtagaaatg atgctggaag ctaaccgcat cggcggtcgt
    28081 cacggcctgg gcatgagcga ccagattgag aaccgtatca tcgaagcgaa aagccgtggt
    28141 atttacgaag ctccggggat ggcactgctg cacattgcgt atgaacgtct gttgaccggt
    28201 attcacaacg aagacaccat tgagcagtat cacgcgcatg gtcgtcagtt gggccgtcta
    28261 ctgtaccagg ggcgttggtt tgactcccag gcgctgatgc tgcgtgactc tctgcaacgc
    28321 tgggttgcca gccagatcac tggtgaagtt accctggagc tgcgccgtgg gaacgattat
    28381 tcaatcctga ataccgtctc agagaacctg acctacaagc cagagcgtct gacgatggaa
    28441 aaaggcgact cggtgttctc gccagatgat cgtattggtc aattgaccat gcgtaatctg
    28501 gatatcactg atacccgaga gaaacttttt ggttatgcca aaactggcct gctctcctcc
    28561 tctgccactt caggcgtgcc gcaggtggag aatatggaaa acaaaggcca gtaattcgac
    28621 atcaaccctg cccttc
//
LOCUS       DAJTNA010000033        28305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTNA010000033 DAJTNA010000000
VERSION     DAJTNA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28305
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.033"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..753)
                     /locus_tag="OO161_001224"
     CDS             complement(<1..753)
                     /locus_tag="OO161_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5462368.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQEFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSTLTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFN"
     gene            complement(753..2336)
                     /locus_tag="OO161_001225"
                     /pseudo
     CDS             complement(753..2336)
                     /locus_tag="OO161_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(2368..3192)
                     /locus_tag="OO161_001226"
     CDS             complement(2368..3192)
                     /locus_tag="OO161_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5462369.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDSLLEEKLYLSIRIKVLMD"
     gene            complement(3452..4705)
                     /gene="amiC"
                     /locus_tag="OO161_001227"
     CDS             complement(3452..4705)
                     /gene="amiC"
                     /locus_tag="OO161_001227"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5462370.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            4937..6268
                     /gene="argA"
                     /locus_tag="OO161_001228"
     CDS             4937..6268
                     /gene="argA"
                     /locus_tag="OO161_001228"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5462371.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(6330..8156)
                     /gene="recD"
                     /locus_tag="OO161_001229"
     CDS             complement(6330..8156)
                     /gene="recD"
                     /locus_tag="OO161_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5462372.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(8156..11698)
                     /gene="recB"
                     /locus_tag="OO161_001230"
     CDS             complement(8156..11698)
                     /gene="recB"
                     /locus_tag="OO161_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5462373.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVTSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVVTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(11691..14579)
                     /gene="ptrA"
                     /locus_tag="OO161_001231"
     CDS             complement(11691..14579)
                     /gene="ptrA"
                     /locus_tag="OO161_001231"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5462374.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(14755..18123)
                     /gene="recC"
                     /locus_tag="OO161_001232"
     CDS             complement(14755..18123)
                     /gene="recC"
                     /locus_tag="OO161_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5462375.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(18136..18459)
                     /locus_tag="OO161_001233"
     CDS             complement(18136..18459)
                     /locus_tag="OO161_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5462376.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(18444..18851)
                     /locus_tag="OO161_001234"
     CDS             complement(18444..18851)
                     /locus_tag="OO161_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5462377.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQLAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            complement(18848..19411)
                     /locus_tag="OO161_001235"
     CDS             complement(18848..19411)
                     /locus_tag="OO161_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5462378.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(19402..19872)
                     /locus_tag="OO161_001236"
     CDS             complement(19402..19872)
                     /locus_tag="OO161_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5462379.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(20057..20851)
                     /gene="thyA"
                     /locus_tag="OO161_001237"
     CDS             complement(20057..20851)
                     /gene="thyA"
                     /locus_tag="OO161_001237"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5462380.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(20858..21733)
                     /gene="lgt"
                     /locus_tag="OO161_001238"
     CDS             complement(20858..21733)
                     /gene="lgt"
                     /locus_tag="OO161_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5462381.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIILARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(21884..24130)
                     /gene="ptsP"
                     /locus_tag="OO161_001239"
     CDS             complement(21884..24130)
                     /gene="ptsP"
                     /locus_tag="OO161_001239"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5462382.1"
                     /translation="MLTRLREIVEKVASAPCLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLWRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(24143..24673)
                     /gene="rppH"
                     /locus_tag="OO161_001240"
     CDS             complement(24143..24673)
                     /gene="rppH"
                     /locus_tag="OO161_001240"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5462383.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGITPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            25358..26047
                     /gene="mutH"
                     /locus_tag="OO161_001241"
     CDS             25358..26047
                     /gene="mutH"
                     /locus_tag="OO161_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5462384.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLQEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            26116..26829
                     /locus_tag="OO161_001242"
     CDS             26116..26829
                     /locus_tag="OO161_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5462385.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            26967..27185
                     /gene="ygdR"
                     /locus_tag="OO161_001243"
     CDS             26967..27185
                     /gene="ygdR"
                     /locus_tag="OO161_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5462386.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            27293..>28305
                     /locus_tag="OO161_001244"
     CDS             27293..>28305
                     /locus_tag="OO161_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5462387.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPSIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAV"
BASE COUNT         7078 a         7537 c         6791 g         6899 t
ORIGIN      
        1 attaaaatta tcgtcagttg aaatcatata tgatccggtt agcgcaggaa cattaaatgt
       61 tttcgaaatc gaattaaaca ccaccacttt gtcatctttt attgttagcg ttgacgtgaa
      121 actatgatta aaaacaaaat cactatgtat ttcatctgaa cataaccaaa tattatgcct
      181 tgcacagcat gctgcgattc tgtgaagatc gtcttccgac cagaccgttc ctgtcgggtt
      241 atgaggatta cataataaca ccatcttcgc gcttttaatt ttaagctcaa actcttgcca
      301 gttgataacg tagcctctcc cattttcttt catcaaagag gttgttagca tgtttcgacc
      361 attatgctta atcacatcaa aaaatgcgtt ataaacaggc gtgaaaatga gaatattatc
      421 tccattcaat gtcaatattt ctattgcctt agatattgaa tacatcacgc ttggactata
      481 ggtgatccat tctggttgaa tctctgtttt atattgttga tgataccaat tgactatcga
      541 attcagatag tcatcatgct gccaacgact gtaccctaaa atcgggtgtt ccagacgttt
      601 ttttaatgtt ctgatgatag cttccggtat cggaagatcc atatctgaaa tagagaatgg
      661 taatacatct tttacaccaa atctgtcttc acaaaagtcc cattgtgtgc aatatgttcc
      721 tttacgagaa attgaggtat taaaaatatc catcaacata tccccatgta attatccata
      781 tcccgacgta aggcgatgac ttttgtaccg ataatgactt gtaaggtatt ttgatcaagt
      841 ttcacaaccg caattcctcc ggcccttttt attgcagcct catctatcaa tcccatatcc
      901 gcgatagtta aacgcaaacg agttacacaa ttatccagtg atttaatatt ttctttacca
      961 cccagagcct taaccatttg ctccgctgcc tgcaactctg atttatcatg aactgggatg
     1021 ttagctattg cagtttcttc gtgagcgttc agcgtgacag gactggactc ttcacggcct
     1081 acagtcatca ggttaaattt gagaatggtc caacggaaca caaagtaata aatgaagaaa
     1141 taagccactt caacacatac cgctaatggc catcccgttc ttgtgccacg taagacacca
     1201 aagctaataa attgaatcag atcgccagta aagcccatct tcacgcccat atatggcaga
     1261 accatgttag ccaatccatt cataaaggca tggaagacaa acaacagcgg gctgacaaat
     1321 aagaacagaa attcaatcgg ctcgctgacg ccgccaataa ttaccgtgag cacaccggaa
     1381 atcagcaatg ctttgatagc ccttctgttt tgagcatgag cagtgcgata catcgccagc
     1441 gcggctccag ccagaccata ctgaatcatc agtttgccct gctccatttt ccccgccaaa
     1501 tccagcggaa tgaccgcatt ctctttgacg tatgccataa acatgttcag ggtgccgtaa
     1561 tactcttcac cgccaatcac tgccgatccg ccgattgggg taaagcggaa aactgatgtc
     1621 accaagtgat ttaagccaaa aggtatcgtc acgcgttcgg caacagcata gaagaaataa
     1681 ccaacaggac cggaagttga aatccagtga ccaatgagca tgaaaagatt gaaaaatggc
     1741 ggccagataa aaggaatgat cagaccaaat ccactcatca caatcagtgt aatgataggc
     1801 acaaggcgtg ggccactata aaaccctaat gattcaggaa tacgtaagtt aacgatcttt
     1861 ttgctggcga ctaataaccc tgccacaatc ccacccaaca cgctggtatt gtaggactgg
     1921 atcccgagta tgatggtttg cccatgtgtc gacatttggt cagcagctac caataagtcg
     1981 tgctgtttaa gataaaagtt cgttccaaga tgcatcgcca taaagccaat taagccaatt
     2041 aagccagaaa aagcgccata ggctttatca tctttctctt ttaataatcc caaaggtata
     2101 gctatcgcaa acaatacagg taaattaacg aaggcaaaca aaccaaggct aacaataaaa
     2161 tcaagtatgg ttttaattaa cggaatagcc aacactggaa tgaattttgc catatcatcg
     2221 ctggctaaac cacttcccag ccctaacatc ataccacata cacttaacag agcgatggga
     2281 tacataaatg ccttccccag gctctgaaaa aaactctagg atttcttttg tttcatactg
     2341 gttatctcat gaaaatgtta tgtataatta gtccattaat actttgatgc gaatagaaag
     2401 atatagtttc tcttccagaa gagaatcata tttaaaagtt gcgtacagat atctttcaat
     2461 ttcatgaatg gcacgttgta actgaaaatc tttttcaaga aactgctcat agatatcacc
     2521 aatatttttc tgttttattt tttcattaca taacacacga ctagaaaaat atttaaggtg
     2581 catcataaat ctggagtagt taaatgaact tctgtccacc cttctgttat aaacaacttc
     2641 aataagctcc gtgatttttt gagacagctc aataatcctt actgattcat aatcaactga
     2701 attttcataa ttatttaaaa tatgcaatgc tataaaacca atctcatcat ccggtagttg
     2761 aacgctaaat tgctcattaa tgattgttaa tgcatcacga cttattgcat actctgcttt
     2821 gtacaacgct ttgatttcct caaggaaaac attattaatt tgtatgcctg agacgactcg
     2881 ggatattgca ttcgaaatgt gatcagcaag aatcagtagt agcgatggat tgagtttttt
     2941 ttcgagatta ctctccgcat aatgcactat cttttcggca acaaaaatat actcaatacc
     3001 cacatgctca atcaatttgt agagtttatt tttttgctca tttctgacat aaaaaatccg
     3061 gtttgcagga tgctcaggga ccgacatacc atatttttta ttgtaaccaa cgccaggccc
     3121 agaaatgata acctcctgtc cgttcaccga tgcctgtaca caattattgt tcatgacttt
     3181 ttcgatgatc attccatggc tcccaaaaaa aaaggcaagg catgtcagaa ataataatta
     3241 cttcaaacat tgccttgcct gatttaacag ttacaagata attaactaat gcgtatacct
     3301 tacacggaat tgtgcttaat aaaagagatg acagacttat tttttaagcc gatattcaaa
     3361 tattcgacag cagtcacaaa accatcgttc aacatttatg taaataatat ttattcaatg
     3421 aaacataaat tcattgaata aattaaaaac atcatcccct tctcgccagc gtcgccccat
     3481 cggcaaaata cgctttaatc cccgcaagaa tagactccgc aacttcctgc tggaaagtcg
     3541 ccgttttcag tttacgctct tcctcaacgt tactgataaa cgccgtttcg accagaatgg
     3601 agggaatatc tggtgccttt agtacggcaa acccggcctg ttcaacttga tttttatgca
     3661 gtttgttgat tttaccgagc ttattcagta ccgctttacc aaacttcagg ctgtcggcaa
     3721 tggtcagcga ctgcaccata tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc
     3781 tcacgccacc aatcaagtcc gaggcgttct gggtttgtgc cagatatttt gccgcagtac
     3841 tggttgcacc tttggttgag agcgcaaaca cagaggaacc gctcggctga cgactggtaa
     3901 aggcgtcggc atggatagag acaaacaggt cagcacgctg tttctgggct tttgctacgc
     3961 gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc atattgccct
     4021 ctttctcgat cagagagcgc agacggcgag ctatttgcaa taccacgtct ttttcgcgcg
     4081 ttttgtattt ccccaccgca ccggagtctt cgccgccgtg gccagggtca agcataataa
     4141 caataggacg atcgcgccct gctttacccg gttgtggacc actttgtgct ggcggcacct
     4201 gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat
     4261 cctgtgcatt ggccggatag aggtccatca ccagacgctc tttaaacccg gcgaccggcg
     4321 caagggcaaa cagctgcggt tttacgtttt gctttaattc aaaaaccatc cgtacggtct
     4381 gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg
     4441 ccgccatccc cttgagtacc gagttcaggt ttacatcttc gatatccacc accacgcgtt
     4501 cagggttact caacgcgaac tgcttatatt tcagctgacg atttgattct accgtcacac
     4561 gggtgtagct ggacgcaggc cagacgcgca ccgccacgac ctggctgacc gcagccagac
     4621 tgacctgact tacgctcaat agccacatgg cacccgcgcc ttgcagtaaa cgacgacggc
     4681 tgattgcagt gttggatcct gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa
     4741 caagtaaacg actaatttga ccggaaactt tagcgaaaga cgcataatct gtcatctaat
     4801 aaacggtaaa cattcttttt atattcacgg cattactgat aaaaaagtcg ctctcgcata
     4861 aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat acactaattt agaataataa
     4921 tgcaaagggg tgtgccgtgg taaaggaacg taaaaccgag ctggtcgagg gattccgcca
     4981 ttcggttccc tatatcaata cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga
     5041 agccattgag catgagaatt tctccagtat cgttaatgat atcgggttgt tgcacagcct
     5101 cggcatccgt ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa atctggccgc
     5161 gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg accgacgcca aaacactgga
     5221 actggtgaag caggctgcgg gaacattgca actggacatc actgctcgcc tgtcgatgag
     5281 tctcaataac acgccgctac agggcgcgca tatcaacgtc gtcagtggca attttattat
     5341 tgcccagccg ctgggcgtcg atgacggcgt ggattactgc catagcgggc gtatccggcg
     5401 gattgatgaa gacgcgatcc atcgtcaact ggacagcggt gcaatagtgc taatggggcc
     5461 ggtcgctgtt tcagtcactg gcgagagctt taatctgacc tcggaagaga ttgccactca
     5521 actggccatc aaactgaaag ctgagaagat gattggtttt tgctcttcac agggcgtcac
     5581 taatgacgac ggtgatattg tctcagaact tttccctaac gaagcgcaag cgcgggtaga
     5641 agcccaggaa gagaaaggcg attacaactc cggtacggtg cgctttttgc gtggcgcagt
     5701 gaaagcctgc cgcagcggcg tgcgtcgctg tcatttaatc agttatcagg aagatggcgc
     5761 gctgttgcaa gagttgttct cacgcgacgg tatcggtacg cagattgtga tggaaagcgc
     5821 cgagcagatt cgtcgcgcaa caatcaacga tattggcggt attctggagt tgattcgccc
     5881 gctggagcag caaggtattc tggtgcgccg ttctcgcgag cagctggaga tggaaatcga
     5941 caaattcacc attattcagc gcgataacac gactattgcc tgcgccgcgc tctatccgtt
     6001 cccggaagag aagattgggg aaatggcctg tgtggcagtt cacccggatt accgcagttc
     6061 atcacggggc gaggttctgc tggaacgaat tgccgctcag gcgaagcaga gcggcttaag
     6121 caaattgttt gtgctgacca cgcgcagtat tcactggttc caggaacgtg gatttacccc
     6181 ggtggatatt gatttactgc ccgagagcaa aaagcagttg tacaactacc agcgtaaatc
     6241 caaagtgttg atggcggatt tagggtaagc gatgaaaatt gtcggatgcg acatgcgtaa
     6301 cactcgtgcg tcgcatccgg caattacgtt tattcccgcg aactaaacaa cgccgccaga
     6361 ccactgcgcc gctcagtacg agtggcgatt gccgcactta atatacgctc atcggcatac
     6421 agcgacagac ggcgacgcgc gcgggtcacc gcggtgtata ccagctctcg cgttactacc
     6481 ggcgtgcgtt ggctcggtaa aatcaacgcc gcatggtcga actccgatcc ctgcgattta
     6541 tgtaccgtca tcgcccacgt agtttcgtgc tctggcaagc gactcggttg cacagactta
     6601 atattgccgt ccggcatcgc aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata
     6661 ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac gggcaatcat caccggtcga
     6721 ccttcgtacc aacgagagtg cggatgacga tgaattttgc gcttctgttg cataaactgc
     6781 tcaattcgct cattcagtcc agccacgcca aacggccctt cccgcagggc gcacaaaagt
     6841 tggtactcat tgaacgcctg aatgattaaa tccggctcgg cacgcgcttg cagcagatcc
     6901 agataacgtc cataacccgc aagagcttcc tcaagcatcg caatataatc ttcgccgctc
     6961 tgtaaaagtc gtttttcgat atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt
     7021 ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct gccgaaacga
     7081 tagctttttt gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt gccggttcct
     7141 gccggaacgt gagtccccgt caggcggctt agctgcctgg cacgctcggc ggtaaagccc
     7201 gcgttggcat aagcgcagat atcgcccagc acagccccag cctcaaccga ggccagttga
     7261 tcacgatcgc cgagaaagat cactcgcgca tgatcgggca aggcgtcgat cagtctcgac
     7321 atcataggca gatcgatcat tgacgcttca tctaccacca gcacatcaag atgcagcggg
     7381 ttaccggcat gatgacgtaa acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa
     7441 gtgctggcat cttccggaat gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa
     7501 gccttgccga gagattcggt taagcgcgcg gcagctttac ccgttggtgc agccagacgg
     7561 atacggcagc gttcgccgtc ggccatttga attaacgctg ccagcaactt cgccacggtg
     7621 gtcgttttac cggtgcctgg gccgccggaa atcaccgaga tccgccgcgt cagcgccact
     7681 gccgctgcaa ctttttgcca gttaatttca tcgcttactg gaaaaagttt gtccagggtt
     7741 tgcgccagta gagcttcatc aacctcaatg gcatgattca cctcattgaa gaagcgcgct
     7801 accgtccgct cgttacacca catgcgattc aaataaagac gatcgccaca gaggatcatc
     7861 ggcgtgggtt catctcccct gctgaccgct tgagaagcca gcaagcattc ttcccaattt
     7921 tgtagctcac cgatttcact gacacaggtc gccaacagcg gatgcgacgc ctcgttattt
     7981 tccagtcgtg aaagcggcaa acagacgtgt ccctctccgg catcatgact taacagtgcc
     8041 gccgcgaggg tgacggcagg atgttcatct cccgccacgg tcagggcaaa ttgcacatcc
     8101 agcgggcgta gctgtttgtg ctccacagct tccagtaatt gcttttgcaa tttcattacg
     8161 cctcctccag ggtcataccg gcaaacatct catccatcag ggcaatcaac ccggcgttgg
     8221 gtcgggttgt gtaaatccct tgttgcggat gttctttatc aacgccacgc aggaacagat
     8281 agataacgcc gccaaagtga cgatcatagt cgtaatcagc aatgcgatgg cgcagataac
     8341 gatgcagcgc cagggtataa agctgatatt gcagatcata gcggtgtgcc tgcatcgctg
     8401 ctgccatagc ctgttgggtg taagccgaac tgtcttcacc cagccagttg gatttatagt
     8461 cgagcaggta ataacgccct tcgtggcgga atactaggtc gataaagcct tttaacatgc
     8521 cgcgtacctg catgaactcc agcggcgggc agccagcgga tagcgggtca aactggcgaa
     8581 ttagtgcatc aagctggctg gcgatgagcg gttcgctaat cggcaggtaa aactccatct
     8641 ccacctgttt atcgcggtcg gaaagctgac tcaggctaac acccttttca ttgagaggtg
     8701 cctggaggac agccgtgatc cactcggtca ccaccggttc ccactgcgat tcaaagccgc
     8761 cgagttccag tttttcctgt acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt
     8821 caaacaaact gtgcaagaat gtccccggtg acgcaccgcg cgggaactga tgcggtgtta
     8881 acgtcggttc ttcaacgacg ctgaccacgc ctgcggcatc aacatccagc cgtggcatca
     8941 gatcctgagc gataccgtga ccacgctgct gcaagccgga gtagctggtg acacgccagt
     9001 tatcgccggg caatcgttgt agtgtcctcg cattcagttc tgcagttaaa gcatcattaa
     9061 cctgccaggg ctggttatca ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata
     9121 acgcttcaat acaggtgcga agccctgccg catcttgcgg ttcccctttt tgcagcaaac
     9181 gcccgagcgc actttggtgg acgtcggtgt cacctttttt atcgccacga cggcgcacca
     9241 gcggtgcaac gccgagactg caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac
     9301 gcagatcttc cgccagacgt tcgacctccg cgaggtcgac gctttttggc gcagcattaa
     9361 gatccaaaac tgcctcaaac gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga
     9421 aattggtaat aaatggcaac cagaccagcg gatattccag ccctttcgat ttatggatcg
     9481 tgacaatctg caccagatgt ttatcacttt cgaggcgcat ttgttggctg gaggcattac
     9541 tgtctggctc gaggatatgt tgcgataacc agcgtaccag cgcatgttca ctttccagct
     9601 gcgttccggc ttcttgtagc agttcgctga tatgcaagat atcggtaaga cgccgctcac
     9661 cgcctgccgt tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca
     9721 tcggcataac gccacgtttg cgccagattt gccgataacc atcgaactct tcaactaccg
     9781 catcccacgc atgttcgtca ttattcagcg tttcgatatc cagcgcgttc agccccatca
     9841 ttgacgttgc cagcgcacta cgcagggtgt tctcacgttc gggcgtcatc accgcctgca
     9901 ataaccaaag catttcctgc gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt
     9961 aaacggaagg gatttccagc aacgttaagg catcgcgcac ctgggcggcc tcctggcggc
    10021 tgcgcaccag cacactgatg tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca
    10081 gcaacgcttc gccccgctgt ccggcttgta gccagtcgcg gatttgcgca gcacatacct
    10141 gcgccatggt actttgatag tcgccaacgc cgcagctttc gccttccatc agccacattt
    10201 tcatcgcagg ctgcgtttca cctttaaata caaaacgtaa cgcctgattt ttcccggctg
    10261 atgtcactgg aataaacggt atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa
    10321 gcttgttcac actgttcacc attccaggtg cggagcgcca gttggtgtct aaagtgtagt
    10381 gggcgtgaac ttcactacgc gccttcatat aagtgaagat atccgccccc cggaatgcat
    10441 atatggcctg cttcgggtcg ccaatcagca acaatgcggt ttccggctga tggtgccaga
    10501 tacggcgaaa aattcggtac tgctgggggt cggtatcctg aaattcatcg atcattgcca
    10561 ccgggaatcg cgtacggatc gccgctgcca acacctcacc gctttcgcta cgcagcgcgg
    10621 aatcgagccg acttaacatg tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg
    10681 ctactgtttc gcggatctca gccaatgcgc gggtgatcac cagatcgcgg atcgacaatg
    10741 gttctgcaag cagttgatcg atcgcctcaa acagtggatg tcgcggggtt tcccccccgg
    10801 ctttcgtgcg atcttctaag aaacgctggg agaatttttc cagcgactcc ggcaactgat
    10861 aactgttggt ctcttcttct gcccaggcgc tgatcttgtc gatccattta gcctgattgc
    10921 tacggttaaa cttgcgtcga tcaataccag aagattcgat cagcgcatcc agttcaccca
    10981 ctgcgtcgcg ccactgctgt tttaccgtag caatacgcgc cacaatttgc gcgtggcggg
    11041 aagccagcgt ttcatcatcg ggcggcggtg ctttgataac cggcgcttcg ccttgcagat
    11101 aacgattaat atcgcgcagc aacgcctgcg gccctttcca ggtttcaaag acgacctggg
    11161 ctatttcacg cggcagcggg tagcagtggc gacgccagaa atcggcgcag gcctggtaac
    11221 gtagcagaga ctcatcttca atcagctgct gctcaaacag catgccggat tcaaaggcat
    11281 tcaggttgag catgcgctgg caaaaaccgt gaatagtaaa gactgccgct tcatccatct
    11341 gccgttcggc taacaacaac cactgagcgg cttgcgcttt atcgtcgatc tcttccagca
    11401 gacgttcgta cagtggattg tcggtagttt cacgcagaca ggcgatgcgc aactcatgga
    11461 tattgctacg gatacgacca cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca
    11521 gcagttcttc aacggtcagc gggcggggaa aggcggcgga accgcctagt ccaagtaaca
    11581 ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt gccggcagag gcttcaatca
    11641 ggcgctcacc ctgtaagggc aagcgcaaag gatctagtgt ctcggcgaca tcactcattc
    11701 ttttcactca tcaggggcat tgtttgctgc aacgcgctga cgttctccca cactttccag
    11761 ccttcagggt gtacatattc ggctttcccg ttctggctgc cggaaatctg cgacagaata
    11821 gccatgcctt gcggctcgac caccgcctga tggaagaaat cagcaagttt ttgcggcgtc
    11881 agcagtttta tctgggccac gattttatca cgcgaatcga agcgcatatt gccgcgatcg
    11941 aaatctttac ttaacttcga tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg
    12001 gtaattaccg cctgctggat ttgcgcaaac tcatctggct tcatcgctcg caattttgcc
    12061 tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga atgaaggctg tttatcattg
    12121 ctttgcaaaa ggaagcccat gccccactga cgccccacgc tcattggaaa cgcaaacacg
    12181 gcatagccca attgttcttc ggtacgcaac tgattgtaga accacggctg tacgatctgc
    12241 cccaacagag agctataggc tgagctggtg tattcatcgt agccagtcgg tacaaatacc
    12301 gctgccagtg cggagtcggt gctgttaccg gctttttcaa agatgacgga ttgtttttta
    12361 tcgaccacta catctttgtt tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc
    12421 acatcgcgtg cctgcgttgt tgcctgggcc tcggtcatgt tgccgataac cataaactct
    12481 ggtcgagccc ctgattttaa ggcgtcgcga taggccagca cctctttcaa cgtaatggag
    12541 ggcaaaattt tacgccgttc atctcgcgag aagtacggca cttgcgagag catctgcgcg
    12601 ggcataatcg cctgctcaaa cgctttaccc ttttctgcgg aatccatcat ctggttatac
    12661 caagacttcg cctgctcaag ctgatcttcc gtagcggtat agctaaagta cccctcgagc
    12721 aatgcctgga acagctgcgg cagacgctgg gtgtaaccat tagcattaac cataaggccg
    12781 ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg cctggttgct taactgatca
    12841 agcgccagcc ctgcgagata atcattgagc gcaaacatca cctgattgcg ggcgctgtcc
    12901 atggctttcg gattacgcaa aatcaggctg acatcagctt tgggttcgct ggaaaaataa
    12961 cggcttggcg catacaccac gcgcagattc gactcatcaa caatcagctc tggatggtca
    13021 tatttcttct ctgacttaat cagcgagaaa tcgtcaggaa tataggggtt aagctccggt
    13081 aaggagagcg caatattggc agctttttgc tgccagtcag cgaaagtttg ttcgctgatt
    13141 ttatcgacct gatacggcgc atcgacaaaa taagccgttt tattgtgcgg ctctttcggg
    13201 ctgatatacc agatacgcgc attctgcggc gtcatcatcg ccagacgttc ttttactgct
    13261 ttagcatcgt accgatcggc aatattgact gcatccagcg tatgctcaac cggaacgcga
    13321 atcatggtat ctgccagcca ttcgacgtaa tccatatcac gggtgatcga cggataacgg
    13381 aagtcgatat ccagcacatt cgccagttca tcgaagtatt gtttatcaat gcctttttca
    13441 cgtaacagat tgagatagct aaaaattgcc gccacaacct gatcgcgatt agccaggcct
    13501 ttatcggtta aagacgcaga gatcgctaat acgccgctgt tgccgttgac gataggatcg
    13561 gagttggcgc taatgccctc aactaatccc tgcttttgca gccagtcgga aagtgtacct
    13621 gggctgcgat taccaatcag ataggtaatc aattcatccg ttttactacg gaacttcgct
    13681 gagttgttat cgatgcgaaa ctcaacgcgc agaactttac gcggcagtgc cgggacgtaa
    13741 tgaatgataa tgcccttttg cgcgtcggtg actaccggca cggtgatttc cggttttttg
    13801 ctctctttgt tcggcacgcg accaaaggtg tccgccgcca tttttgccaa ctccggcagc
    13861 ggtttattgc tgtaaataac cgccttcatc agattggcgg aatagtactt ctcgtggaaa
    13921 tctttcagcg cctgctgcac cggattacca ggtttatcgc ttaaagtttc gaggttacca
    13981 ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg
    14041 cgcatcccgt cacgcgtacg cgccatggtt aattcagcgt tcaccgcatt acgctcacgt
    14101 tcggcatatt tcttgtcgag caaaggttca gcaatagcat cggccaggcg gtctaccgca
    14161 ccaggcaagg cgtcgttctc aacttccaga tagaaagccg tgcgatacgg cgcagtgctg
    14221 gcattgtgac taccgccgtg cattttgaga tattcggcca gactgtcagc ctgcgggtac
    14281 tttttcgacc ccatcagact catatgttca aggtaatgtg ccagcccctg gtacgcctcg
    14341 ggatcttcca gcgacccaac gggcaccacc agcgccgaga gcgatttaac tgcctgcgga
    14401 tcagaaacca gcaagaccac cataccgtta tccagacgta tagcctgata ctggcggtta
    14461 tctttatcac ttttacggat ggtttcctga atcggctgcc atcccgtttc tgcctgactt
    14521 aagggtgccc aaagggcaac taacaacaat aatgctttga accaagtgct gcggggcatt
    14581 cacggacctc ataatcaact taattttctg tccagattca acacgttaac gcaaatcatc
    14641 tgccagaatt taatcttgtg ctgcacgagt cagcctatgt ttatataacc atcagtccgt
    14701 gactggtgcg catcataaag taagcggata gattgcgcaa tttttataca gcactcatga
    14761 ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga tggcctccat
    14821 tgtctctggt gttaattgcc gccagagcct ttgataccag atatcatcac cttcgccacg
    14881 caccatcatg ttgccttcgt aagcctgaag gaatttcgta cgggcttttt gcaacgtgga
    14941 atcgtcatcc agcatggcat cgttttgcgc gtcataacag gtttttagcc acgcgccgcc
    15001 actttcaggt aacaccagca atggcgcgga catcccttca cgatacccct caatcagttg
    15061 tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga aaacgccact cgccgtcttt
    15121 gcgtagaaaa aggcgacttt caccattacc accgctggca cagtagacaa ggtgttccag
    15181 ccaaagttgc attccctgcg ccacacttaa taaagaggga cgccagcgca acaggccatc
    15241 cggctgcacc tgcggcaacc agccagttat ctgcacaccg ttgcaggcga gatcaatttc
    15301 catactctgc cccggctggc gacaggcaat gactctgtcg gcaagctgct gcatctcctg
    15361 gcactgagtt tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg
    15421 gaagcgacgg aacaagcgtt cggcatcatc ctgctcaacc agtgcattca ataactgctg
    15481 attgatttga taacggctaa gcccttccag aataaatggc tcggtgtcgg ggatttcgct
    15541 gtcttcggta cggaagttca cctgcaaacg catctggaaa aatgcccgca ccggatgtgc
    15601 ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg gcagcggctg
    15661 aacaaattca gaatgtgctt taccagcctg gctggccgca ggtagccatt cacgagcata
    15721 gctttgtcgt tcgcctggct ggtagttttg tggatcaaac ggcatccggg tatggaggca
    15781 ggtaagatgc gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg
    15841 tagataatga ctttgcccga tgtagtcgat cagttcctgc accagtaccg acgggaaacg
    15901 ttcactgtta tcctgaatgg aaagcccgat ataactgata tagagttttt gctgcgcgga
    15961 aattaacgct tccaggaaca ggtagcggtc gtcatcgcga cggctacggt cgccacgctt
    16021 cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc
    16081 gttcattccc agcaggcaaa ccactttgaa cggaattgaa cgcattggca tcagagtaca
    16141 aatgttaacc ggtccggcga gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc
    16201 cagttcatca cgcaatagtg acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc
    16261 ggcgataatc gcctgccatt gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc
    16321 cggcaggaag aaggcgttga gcatatcgcg acaaaccggc aaccactctt ctagcggacg
    16381 ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt agcagtgaag ccagatgccc
    16441 caccagttct gcaattaagc cgctcgactc atcataaggt agaaccgatt gccactcgcc
    16501 ctgcgcgctc tccatcgcgt agcccagcaa catacgcgtc aggccaaatc gccaggtgtg
    16561 ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat
    16621 gccggattcg ttgacccact ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg
    16681 cgccgccagc accggcacat ccagtaatgc cagcacgtct tccgacacaa agcggctgtc
    16741 tggcaacgat aacaggctga taaacgcttc cagtaccgga tgcgactgcc gcgcccgacg
    16801 gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca cagcctgaat
    16861 aaacggactg tagctgtcga tatcagccac catcacgatg atgtcgcgcg gagtaagtgt
    16921 cgggtcttct tccagcatcg ccagcaggcg atcgtgcaaa acttcaactt cacgctgcgg
    16981 gctatggcaa acgtggaagg tgatactgct atccagtgga tcaagcgggc gtttgttatc
    17041 gctacgggaa aactcttcga tgttcacacc agcaacggca cggttttcca gttccagaat
    17101 gtcagactga atgttatgca gcaggttatc tggcgtgaca tcgacaaagg cgtccagctc
    17161 ctggctgctc tccaggtcag aaaggagata aatgtagtcg cgcccaagct tcccccatga
    17221 ggccagcagc gggttgccga catcctgttc accatcgctg ttaaagagct gtccggcatt
    17281 ttcgctatcg cgaaatagcg gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt
    17341 cagcagtttc gccagataag caggatcttt aatatcgccc cagtaataac ggcaggggtt
    17401 ggtaaacagg agatggattt caatatgttt acccagcgcc tggagcgcct ggagataaac
    17461 aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt
    17521 cgtcgcggac tccagcgttt cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg
    17581 ttgtccgagt tcatgggtat attccaccag cgccttccac aacggcgctt gccaggcctg
    17641 tgcttctccc agcccttcaa ccaaatgtcc tgtttcccat tgtgccagcc actccgggcg
    17701 atagaccaga tactggtcaa acaggtccgc cgcttttgag gaaagctgga acagttttcg
    17761 cttgtcgcta tcgtcagtca gataatgccg caacagggta aagtcttcgt gctccaacag
    17821 ttgcggcagc agagtcatca gtttccagct catgctctgt ttgttaaagg cgctctcttt
    17881 ggggatctcc ggtaacaccc ggacgaacat atcccagata aagctcgcag gcagcggaaa
    17941 atcaatgttt gccgcaatac caaacttttg cgacagggtc atttgcagcc actgtgccat
    18001 accggtactt tgcaccagaa tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc
    18061 gacaataaac tccatcaacg cttccagcac gtccagacga ttggaatggt agacccttaa
    18121 catagcggct cctgactact gacgattcgg gcaatgcagg cgggtcatct cgccttctct
    18181 gcccccgggt gaaactagcg taacgctgat gctgacacat cccgcctgcg atgtctgcaa
    18241 tcggttgacc tgccagttag ctggtggcga aatcgcgcgc agttgcgttt gctgccagcc
    18301 atgccgccag agctgttggt actggtttct gctggcaaga ctgttcatta atgttcgctg
    18361 gaaacccgat aacgcagtga caatcatcac catcaacacc atcgccaaca ttacctccgg
    18421 caggctaaag ccttgttgat tcttcaggga agctgacata acgccctctc tttcagcgga
    18481 caaaaatcgc tccagccgct tggcgaaaaa acaatgtttc catcaatgac ttcgcctgtt
    18541 cgccacaacg aaacgccttc ataaccggca atcaataagg cttcattatc tgccagtaaa
    18601 cgcaaacaaa cctgggcatc ggtttcagcg tactgcgagc actgaactgc tagctgcgtc
    18661 tgccaggagt gcatttttcc ccacgccagc gccgactgaa cgatggcctg gcggcgcaat
    18721 gactgacttt ccatgctcac gcgagaggca aaactgcgat cctgttggct cattccttgt
    18781 aatagcaaac tacccaaaac cagcagcatc aggaccagag ccagtgacga aacacccttt
    18841 tcgcggttca caggttgaat cctgtcacgc tatagctggc attcaccacg gtttgcggtt
    18901 cagacttact ggcagcacgc atattaaccg tcaacaccgg cgagaagccg ctgacatcct
    18961 gacgtacgac ctgaaaagtg tcgataatga tggcatccgg attagtgact ttatcccagc
    19021 ccttaccttc acaggatgtc gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa
    19081 atccaatctg gtcagactct tttaccggtt cgcgatccca gataccgtta ctgttcgcat
    19141 cccactgcac aatgatacag tcaccctgtc cgacaatttc cagcccttcg ccggtacaga
    19201 tgccatgaca ataacccgcc ctctggagat gcttcgcgac ggtaaatacc cgcagccaga
    19261 tttcatcttc cagcgccagc ttacgggtgc tcgttaaact ttcacgctgt aacgcaggca
    19321 gaaagcgtgc agcccccagc aacaatacgc tactgatcgc catagcaatc aacacttcca
    19381 gcagagaaaa accttgctct tttacaggca tccttctgtt tctccttgct gacaaagccg
    19441 gagtcttccc cacggcgaaa ccaccagcca ccactcgccc gttgagtttt tgaagcgaat
    19501 atgcccggcc catgcggtat tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct
    19561 catttcgact tcgggccagc gtggcacaaa gaccaatggt gaactgccat gacaggtatt
    19621 ggccccagca gcggaactca caaggcacca taacgtcccc tccctgataa cgctgatact
    19681 gtggtcgcgg ttatgccagt tggcatcttc acgtaaatag agcaaatagt cccgcgcctg
    19741 gctggcggtt tgccatagcc gttgcgactg ctgccagtat tgccagccat agagtccact
    19801 tgcgcttagc atgaccaaaa tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg
    19861 ttgtgttttc atgccggcag tatggagcga ggagaaaaaa agacgagggc cagtttctat
    19921 ttcttcggcg catcttccgg actatttacg ccgttgcagg acgttgcaaa atttcgggaa
    19981 ggtgtctcga agaatttaat ggtgggtaaa aaaaaccgac gcacactagc gtcggctctg
    20041 gcaggatgtt tcgtaattag atagccaccg gcgctttaat gcccggatgc ggatcgtagc
    20101 cttcaatctc aaaatcttcg aaacggtagt cgaagatgga ttcgggttta cgtttgataa
    20161 tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag atgagtttga tccatatgat
    20221 tgctgtacag gtgcgtgtcg ccccccgtcc agacaaaatc gcccacttcc agatcacact
    20281 gctgcgccat catatgcacc agtaacgcgt agctggcaat gttaaacggc aggccgagga
    20341 aaacgtcaca ggagcgctga taaagctggc aagagagttt gccgtctgcc acatagaact
    20401 ggaagaacgc atggcacggt gccagcgcca ttttatccag ttcgcctacg ttccacgctg
    20461 aaacaataat gcggcgcgaa tccgggtcgt ttttcagctg gttcagtacc gtagtgatct
    20521 ggtcaatatg acgaccatcc ggcgttggcc aggcgcgcca ctgtttacca tacactggcc
    20581 cgaggtcgcc gttttcatcg gcccattcgt cccagatggt gacattgttt tcgtgtagat
    20641 aagcaatgtt agtgtcgccc tgcagaaacc acagcagttc atggatgatg gaacgcaggt
    20701 ggcaacgttt agttgtcacc agcgggaatc catcctgcag gttaaaacgc atctgatgac
    20761 caaaaatgga aagcgttccg gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca
    20821 ctttttgcat cagttctaaa tactgtttca tggttcctca ggaaacgtgt tgctgtgggc
    20881 tgcgacgata tgcccagacc atcatgatca cacccgcgac aatcatcggg atggaaagaa
    20941 tttgtcccat gctgatgtac tgcacccagg caccagtaaa ctgcgcgtcg ggctggcgga
    21001 aaaactcaac aatgatgcga aacgcgccgt aaccaatcag gaacaaacct gagacagctc
    21061 ccattgggcg cggtttacga atatacaggt tgaggataat aaacagcact acaccttcca
    21121 gcaacagctc gtaaagctgt gatgggtggc gcggcagcac accgtaagta tcgaaaatgg
    21181 attgccactg cgggttggtt tgcagcagca aaatatcttc tgtacgggag ccagggaaca
    21241 gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc
    21301 ccagacgccc ggcaccaaga ccaaacggaa tgagtggtgc gataaaatca gagacctgga
    21361 agaaggaacg tttagtacgg cgggcgagga taatcatcac cacgataacg ccaatcaggc
    21421 cgccgtggaa ggacatgccg ccgtcccaga cacggaacag atacagcgga tcggccataa
    21481 actgcgggaa gttgtagaac agaacataac caatacgtcc accgaggaag acgccgagga
    21541 agcccgcata gagtaagttt tcaacttcat ttttggtcca gccactgccc ggacgattcg
    21601 cccgacgtgt tgccagccac attgcaaaaa tgaaacccac cagatacatc aggccgtacc
    21661 agtgaagcgc cacgggtcct attgagaaaa tgaccggatc aaactccgga aaatgcagat
    21721 agctactggt catctgtcac cacaagttct tgttatttcg ctgaaagaga acagcgattg
    21781 aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa taatagcaca aggggacctg
    21841 gctggttgcc ggataccgtt aaaagatatg tatatgatcc gcgctataac cctccgcgaa
    21901 tcagcccgcc catgccgcga cgctccataa aggctgcaac ctgatgacga acttcggtcg
    21961 ccagttgcgc ttccagacta cgctgcgcaa gattttctgc ttcggcaaaa tcaatgcgcc
    22021 acagcaggta ttttacccgc gctacagaac gtccgttcat agacagatgg cgatacccaa
    22081 gcccaatgag gattgccacg cacatgggat cgcccgccat ttcaccgcac aaacggagat
    22141 cgataccatg tatttccgct tcccgggcga tcattgccag agctcgtaac attgcaggat
    22201 gaaggctgtc ataaatgttc gccacccggg tattgttgcg atcaacggcc agaatgtatt
    22261 gagtcagatc gttggtgcca acagagatga aatcgacccg ctttgccaga tgcggcagca
    22321 taaataccat tgacggtact tccagcatga tgccgatacg tggtttggga atttcgtaac
    22381 cgatcatctc ctcgacttca cgtccggcac gttcaatcag gcggcgtgct tcgtcaactt
    22441 catcgaggct ggtgaccatc ggcaacagaa tattcaggtt gcccgtagcg gcattagcac
    22501 gcagcatcgc ccgcacctgg atcaagaaga tctccggctg atcgagcgta atgcgaatcc
    22561 cacgccaacc caggcatgga ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg
    22621 ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt gaacatttgc agcatcccct
    22681 gatactgcgc cacctgttct tcttccgacg ggaaaccact ttgcagcatg aatgggattt
    22741 cagtgcgata aagaccgatg ccatcaatac ggctgcccag tttttcctca tgttccgggc
    22801 ttaaaccagc attgagcatg acttttatac gctcaccgct ttttaactga gcgggtaaat
    22861 tgacgtcatc ttccgccaga cggctaagct caatctcttc actaattagc cgctgatatt
    22921 cttgcagcag taccggctcc ggatcgacca gcaattcacc gcgatagcca tcaacgatca
    22981 gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc catcacggta gggatcccca
    23041 gcgcacgtac catgatcgca gcatgggagt tggctgcacc atctcgcacg acaacaccga
    23101 ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga cagttcatct gccaccagaa
    23161 tgaaacgttc cggccaggcg ttcggccctt gattagcgtc atcaagatga aacagcaatc
    23221 gctgacccag cgcacgtaaa tcgccagccc gctctttgag atagttatcg cttagcgcgg
    23281 caaactgttc ggcaaatttt tcaatgaccg tttttaccgc ccactctgcc accgagcctt
    23341 tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc cgaaagcagg tgcgagtaaa
    23401 gatcgaaaat agccgccgtt tctttttgtg ctccggcggc aaagcgtttg ctgtagcggc
    23461 gaaactcgtt tgccgcttct tccagcgccc cggtcaatcg ttcgcgttcc agagccggat
    23521 ccagcgttga tgcctgatac acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt
    23581 cggcaatcgc cacaccaggt gctgccggta atgcgcggat tcgcgtctgg cgatattgcc
    23641 caaacaaggc agtcaactgc gactgagaaa gaatagctgc catctgggtg gcaagcgtca
    23701 ccaggaagga ttcttcactt tcgtcatact ggcgcaactc tcgttgctgt accaccagta
    23761 caccaagcaa ctggcgacgt tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt
    23821 cttttacgga ggggatgtat ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg
    23881 gttccgccag cctgccaacc aggccgacga tcccttcatc aaacgcgagc gttacagtgc
    23941 gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat
    24001 cggccaggta gaccgaacag acctcggtat ccatcgcaag acagatgtcg gtaaccagaa
    24061 tatttaacgc ctcattcagg catggtgcgc tggctacctt ttcgactatt tcgcgcaggc
    24121 gagtgagcat aattggcgtg acttaacctc ttttacgtcg ataagcagat gtgttttgtg
    24181 gttttggcgt attttcctgc agtgacatca ccacactcgc gaactctttc attaccctac
    24241 ggtagacatc acgtttaaat gacaccacct gtctgaccgg ataccagtaa cttacccatc
    24301 gccagccgtc aaactctggt gtactgctgg tttgcatatt gatttctgca tcgccgctca
    24361 ccagctgcaa gagaaaccat ttttgttttt ggccgataca aaccggcttc gtgtcccaac
    24421 gcaccaaacg tttcggtaat ttgtagcgca accagttacg cgttgaagca aggattcgaa
    24481 cgtctttgcg gcttaatcct acttcttcaa acaattcacg gtacatcgcc tgctctgcgg
    24541 attctccggg ggtgattccg ccttgcggaa attgccagga gtgctgacca aatcgccggg
    24601 cccacattac ctgcccctgg cgattacaaa tcacgatacc tacgtttggg cggtagccat
    24661 cgtcatcaat caccggacta cctcaaaata aagctttata tacgaatgat tgtttcatac
    24721 tccaggaaga cggtaaacca ctctctgcag ggcattacac actaataaca attgaataac
    24781 tcacagttat gtgcagagtt ataaacagag gaaggggtgg atagccgttt ttattcacct
    24841 tttctgtgga tagagttgtg aagaactacg gaattactac gggaaaaccc ggagcattct
    24901 gaataagccc gatataggca ttttaaaata ttcattatca gtcaatgcat tacatgtaat
    24961 ttgagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta cgggttggcg
    25021 aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag cactgaacca
    25081 ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt
    25141 tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta ttttcagtga
    25201 ccagatttgg aaaacctatt gcagtgctgc gcaactcgat tatcggcagc ctaaaaagca
    25261 atataaatca gagaattgaa caacacatgt ggaaaaagtt acactgcgaa tattcggcac
    25321 ataattgctg tttgtttttt aaccaaggta tcatgacatg tcccaacctc gcccactgct
    25381 ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt ctggttatac
    25441 attgggagaa ctggcggcac ttgccgggct ggttacgcca gagaatttaa aacgcgataa
    25501 gggctggatt ggcgtgttac aggagatctg gctaggtgcc agcgcaggga gtaaacctga
    25561 gcaagatttt gctgctctgg gtgtggaact taaaactatc cctgtggata gtcttggtcg
    25621 tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg
    25681 ggaaaccagc cacgtgcgcc acaagctcaa acgcgtactg tggataccgg ttgaaggcga
    25741 gcgcagcatc ccgctggcgc agcgtcgcgt aggatccccg ttgctgtgga ccccgaatga
    25801 agaggaagac cgacagctac gcgaagactg ggaagaatta atggatatga ttgttctcgg
    25861 tcaggttgag cggatcaccg ctcgtcacgg ggaatattta cagataagac cgaaagcagc
    25921 gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc tgacgctgcc
    25981 acgcggcttt tatttgaaga agaatttcac cagtgcacta ctggcccgtc attttctgat
    26041 ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct tcattttttt
    26101 ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc
    26161 ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt cctttctctg
    26221 gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg gttggcggga
    26281 gccatggtta tgcgtctggc gctgctggca tcaatcgcct gggttacgcg cctgacaaat
    26341 ccgcttttta caatattcag tcaggaaatt tctgcccgtg atttgattct gcttctgggt
    26401 ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga aggtgaagaa
    26461 gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg
    26521 gatattatct ttagcctcga ctcggtgatt acggctgtgg gtctgtcaga tcacctgttt
    26581 attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt
    26641 ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctttcttt cctgattctg
    26701 gtgggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg ttacatctac
    26761 ttcgcgatgt tcttctctat tgcggttgaa agcctcaact tgattcgcaa caaaaagaat
    26821 ccgctctgat actccgtacg ctctcctgct ggagggcgta ctcactcctt ttaccctcac
    26881 agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag actattatca
    26941 taggtaggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt tctgcagtcg
    27001 gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc aaagatggcc
    27061 gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg gtgagttatc
    27121 acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa attattgaac
    27181 gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc cttttccctt
    27241 ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg ctatgcaata
    27301 tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca cgatgacgtt
    27361 tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg tcgctcaggg
    27421 cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg
    27481 gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg aaaagttaat
    27541 tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc gcccggatca
    27601 ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc gcctacagac
    27661 tgattacctc gatctttatc aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa
    27721 actcggttat agctggacgg attctgcgcc tgcggtttcg ctgctggata cgctggacgc
    27781 actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc
    27841 atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccga gtattgtcac
    27901 cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag
    27961 ccagtatgaa ggggtcgaac tgctggccta ttcatgcctg ggtttcggca cgctgaccgg
    28021 gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta gtcggttcac
    28081 ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg
    28141 tcatggcctg gaccctgctc agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc
    28201 cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa actaacatcg aaagtttgca
    28261 tctggagtta agcgaagacg tattagctga aattgaagcg gtgca
//
LOCUS       DAJTNA010000034        27706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTNA010000034 DAJTNA010000000
VERSION     DAJTNA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27706
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.034"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(28..2217)
                     /locus_tag="OO161_001245"
     CDS             complement(28..2217)
                     /locus_tag="OO161_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCR5462388.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALYQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFWIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2426..2501)
                     /locus_tag="OO161_001246"
     tRNA            complement(2426..2501)
                     /locus_tag="OO161_001246"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2466..2468),aa:Ala,seq:ggc)
     gene            complement(2541..2616)
                     /locus_tag="OO161_001247"
     tRNA            complement(2541..2616)
                     /locus_tag="OO161_001247"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2581..2583),aa:Ala,seq:ggc)
     gene            2837..3196
                     /locus_tag="OO161_001248"
     CDS             2837..3196
                     /locus_tag="OO161_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5462389.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3198..3590
                     /locus_tag="OO161_001249"
     CDS             3198..3590
                     /locus_tag="OO161_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5462390.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="OO161_001250"
     CDS             complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="OO161_001250"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5462391.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            5316..5391
                     /locus_tag="OO161_001251"
     tRNA            5316..5391
                     /locus_tag="OO161_001251"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5349..5351,aa:Val,seq:tac)
     gene            5436..5511
                     /locus_tag="OO161_001252"
     tRNA            5436..5511
                     /locus_tag="OO161_001252"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5469..5471,aa:Val,seq:tac)
     gene            5516..5591
                     /locus_tag="OO161_001253"
     tRNA            5516..5591
                     /locus_tag="OO161_001253"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5549..5551,aa:Lys,seq:ttt)
     gene            5711..6052
                     /locus_tag="OO161_001254"
     CDS             5711..6052
                     /locus_tag="OO161_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5462392.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(6043..6969)
                     /locus_tag="OO161_001255"
     CDS             complement(6043..6969)
                     /locus_tag="OO161_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5462393.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            7059..8057
                     /locus_tag="OO161_001256"
     CDS             7059..8057
                     /locus_tag="OO161_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5462394.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(8054..8272)
                     /locus_tag="OO161_001257"
     CDS             complement(8054..8272)
                     /locus_tag="OO161_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5462395.1"
                     /translation="MEKEQLIEMANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(8274..10289)
                     /gene="ligA"
                     /locus_tag="OO161_001258"
     CDS             complement(8274..10289)
                     /gene="ligA"
                     /locus_tag="OO161_001258"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5462396.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLKALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(10360..11346)
                     /gene="zipA"
                     /locus_tag="OO161_001259"
     CDS             complement(10360..11346)
                     /gene="zipA"
                     /locus_tag="OO161_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5462397.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDYYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            11576..12337
                     /gene="cysZ"
                     /locus_tag="OO161_001260"
     CDS             11576..12337
                     /gene="cysZ"
                     /locus_tag="OO161_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5462398.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            12522..13493
                     /gene="cysK"
                     /locus_tag="OO161_001261"
     CDS             12522..13493
                     /gene="cysK"
                     /locus_tag="OO161_001261"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5462399.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            13877..14134
                     /gene="ptsH"
                     /locus_tag="OO161_001262"
     CDS             13877..14134
                     /gene="ptsH"
                     /locus_tag="OO161_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5462400.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            14179..15906
                     /gene="ptsI"
                     /locus_tag="OO161_001263"
     CDS             14179..15906
                     /gene="ptsI"
                     /locus_tag="OO161_001263"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5462401.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            15947..16456
                     /gene="crr"
                     /locus_tag="OO161_001264"
     CDS             15947..16456
                     /gene="crr"
                     /locus_tag="OO161_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5462402.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLPGSVTVG
                     ETPVIRIKK"
     gene            complement(16498..17349)
                     /gene="pdxK"
                     /locus_tag="OO161_001265"
                     /gene_synonym="thiJ"
     CDS             complement(16498..17349)
                     /gene="pdxK"
                     /locus_tag="OO161_001265"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5462403.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            17454..17822
                     /locus_tag="OO161_001266"
     CDS             17454..17822
                     /locus_tag="OO161_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5462404.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(17825..18736)
                     /gene="cysM"
                     /locus_tag="OO161_001267"
     CDS             complement(17825..18736)
                     /gene="cysM"
                     /locus_tag="OO161_001267"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5462405.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     VLSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18870..19967)
                     /gene="cysA"
                     /locus_tag="OO161_001268"
     CDS             complement(18870..19967)
                     /gene="cysA"
                     /locus_tag="OO161_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5462406.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(19957..20832)
                     /gene="cysW"
                     /locus_tag="OO161_001269"
     CDS             complement(19957..20832)
                     /gene="cysW"
                     /locus_tag="OO161_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5462407.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(20832..21665)
                     /gene="cysT"
                     /locus_tag="OO161_001270"
     CDS             complement(20832..21665)
                     /gene="cysT"
                     /locus_tag="OO161_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5462408.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAVSLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(21665..22681)
                     /gene="cysP"
                     /locus_tag="OO161_001271"
     CDS             complement(21665..22681)
                     /gene="cysP"
                     /locus_tag="OO161_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5462409.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(22985..23776)
                     /gene="ucpA"
                     /locus_tag="OO161_001272"
     CDS             complement(22985..23776)
                     /gene="ucpA"
                     /locus_tag="OO161_001272"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5462410.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNLEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(23905..24762)
                     /gene="murR"
                     /locus_tag="OO161_001273"
     CDS             complement(23905..24762)
                     /gene="murR"
                     /locus_tag="OO161_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5462411.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            24926..25822
                     /gene="murQ"
                     /locus_tag="OO161_001274"
     CDS             24926..25822
                     /gene="murQ"
                     /locus_tag="OO161_001274"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5462412.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            25826..27064
                     /gene="murP"
                     /locus_tag="OO161_001275"
     CDS             25826..27064
                     /gene="murP"
                     /locus_tag="OO161_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5462413.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLHLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDLRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIA"
     gene            27077..27250
                     /locus_tag="OO161_001276"
     CDS             27077..27250
                     /locus_tag="OO161_001276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCR5462414.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            27255..>27706
                     /locus_tag="OO161_001277"
     CDS             27255..>27706
                     /locus_tag="OO161_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5462415.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVKRLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLR"
BASE COUNT         6763 a         7410 c         6901 g         6632 t
ORIGIN      
        1 gataattccc cggttttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa
       61 atacccttgc agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc
      121 gacaaactcc gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac
      181 aatcatcgca tccagcgtgt tcgtgacaat atctttcaca aagacgccat caattttgat
      241 gatatcagcc tgcaaacgct ttaaccgttc gtagttggca tagccggtgc caaagtcatc
      301 aatcgcaatc cagaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc
      361 gttagaaaac gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata
      421 acgcttaaac agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat
      481 taaattgact gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa
      541 caaggattcc agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg
      601 cagaaactta tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc
      661 ataaccttca ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgata
      721 taacgccgtg cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat
      781 ctcctctctg ctatccagcg ctagcacatg atgatgtgcg caggattgct ccgccagcca
      841 gcttaactgc cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc
      901 agcaccatag cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat
      961 gttaaccata tgctggagtc gcccttccgt ttccggcccg ctcagcacta acagcagttc
     1021 actacccggc aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggca
     1081 aattgagcgg atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag
     1141 attatcaatg cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttccaa
     1201 cgcacggaag ttgggcagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg
     1261 attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga
     1321 aatcagcacc gcaagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt
     1381 ctggttgtaa ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata
     1441 gcgaagctta ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc
     1501 aataaagtcg ttttcataag gtgtgcacaa cagaagtaga agcacgctta acgctgccag
     1561 ccagcttaag gtaaatgcgc gtttctcttt gcccaacgac ggcgcgatat ccctgcgcca
     1621 caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga aaagcatgtt
     1681 gtaaatcagc acagcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc
     1741 atcgccgaaa aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat
     1801 gctgcatttg atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata
     1861 tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc
     1921 acacaacacg acaaacgtct ggctaccgag catgaccgca gtctgtaaaa aagagagatg
     1981 aaagttccac agattggtgc aaaacatccc gcataaaacg ggaacgacac ctcgccagcc
     2041 aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact
     2101 ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag
     2161 cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag
     2221 tacactactc tcactgatcg gggttttcag gcacacaaat aataagttta ttcgcatcgc
     2281 gaagcaaaca gggggccagc ctgttgccga tttttcatcc aatgatgctt tcacctggcg
     2341 gcactaaccc ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc
     2401 cgctttgcag cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct
     2461 tgcatgccat gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca
     2521 aatttgctgg gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
     2581 gccatgcaag cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga
     2641 cgggcggcat catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt
     2701 caatcgctga aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta
     2761 ttcatgtcac ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata
     2821 cattcaggga attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac
     2881 cggctatagc cgccagacca ttaataaatg ggtacgcaag gaaggctgga caacgtcacc
     2941 aaaacctggc gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga
     3001 atatattcgc aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga
     3061 tgcgccgctt gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa
     3121 acagtttaca tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat
     3181 tcgcgatagc aaataatatg aaaagattac gcaataaaat gaccaccgaa gaactggctg
     3241 aatgcctcgg tgtggctaaa caaactgtta accgttggat cagagaaaaa ggctggaaaa
     3301 cggaaaaatt tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag
     3361 tttgcgagtt tattcagaac acgccagcct tccataacac accaatgttg atggaagctg
     3421 aagagagaat tgctgaatac gctcctggag cacgcgcccc tgcgtatcga caaatcatca
     3481 acgcgataga taatatgaca gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg
     3541 aaggaattcg caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg
     3601 gcaggatatt atctcctgcc gtttatcttt tatgcgctaa ttactgctgg ttttcgcgtt
     3661 cagcaataaa agccagcgct ttgttgatac gctcgatact gcgggtccta ccaattgcgt
     3721 gaacggtaac atccagtgcc ggagactgcc ccgcaccggt tacggcgaca cgcagcggca
     3781 taccaacttt acccataccc acttccagct catccgccgt cgcctgaata gcgtgatgaa
     3841 cgttttcagc ggtccagtca gtaatcgcgg ccagtttgtc acgaaccact tccagcggct
     3901 gacgcgctac cggacgcaga tgttttttcg cggcgtcggc atcgaactca gcaaaatctt
     3961 cgtagaaata acggcagctc tgtgccatct ctttcagcgt cttgcagcgt tcgcccagca
     4021 gtttcaccag atcagccagc tgcgggccgt tacgggtatc gatattttcc tgctcaatgt
     4081 gccactgtaa gtgagtagca acatactccg gcggcagcgc gttaatgtag tgatggttca
     4141 gccacagcag cttgtcggtg ttgaacgcac tggcagattt gctgacggca ttcagggtga
     4201 agtatttgat catctcttca cgagtgaaga tttcctgatc gccgtgggac cagcccagac
     4261 gcaccagata gttcagcagt gcttctggca aataaccgtc atcacgatac tgcattacgc
     4321 tgaccgcccc gtgacgtttg gacagttttt taccgtcatc gccgttgatc atagaaacgt
     4381 gcgcgtaaac cggcaccggc gctttcaggg ccttaagaat gttgatctgg cgtggcgtgt
     4441 tgttgatatg gtcttcgcca cggataacgt gggtgatttc catatcccag tcatcgacaa
     4501 ccacacagaa gttataggtt ggggaaccat cggtacggcg gataataaga tcgtccagtt
     4561 cctggttgct gaactcgatc ggaccacgga tctgatcgtc aaaaacaaca gaaccttcct
     4621 gcgggttagc aaaacgtaca acgcacggtt catcatcagc gtgatgctca tggctgtggc
     4681 ggcagcgacc gtcataacgc ggcttctcac ctttcgccat ttgctcttcg cgcagcgctt
     4741 ccaggcgctc tttagagcaa tagcatttat aagcagtgcc ctcttccagc atctgatcga
     4801 tcaccgcgtt gtagcgatca aaacgtttgg tctggtagta cggaccttca tcccactcta
     4861 ggctcagcca gttcatgcca tccataatgg cttcgatagc ttccggcgtg gaacgctcaa
     4921 gatcggtgtc ttcaatacgc agcacgaact caccgccgtg gttacgtgca aaaagccagg
     4981 agtaaagagc agtacgcgcg ccgccaacgt gcagatagcc tgttgggctt ggcgcgaagc
     5041 gagttttgat tttcatgaaa tggccttacg tttagaaaga tgccgacaac cggcaaatcc
     5101 tggaaaaatt gagtgggcga tattctatca ctcccgcctg attcctcaat gtagttcggg
     5161 tttctacctt acgcattatt gttttcgttg acaaattgcg caacacggtc tgttttgcga
     5221 tcatcttgct taaatttacg acgaacgaac aatttcttta gaaaatgcgt tgactcattt
     5281 tgaactctcc ctataatgca actccacaca gcggggggtg attagctcag ctgggagagc
     5341 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc aactacttta
     5401 tgtagtctcc gccgtgtagc aagaaattga gaagtgggtg attagctcag ctgggagagc
     5461 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc acttcgggtc
     5521 gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc
     5581 acgacccacc aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg cgatttgtaa
     5641 gattgatttt tcttctcaag ttccttgttc ttctaccgat attacctcgt acagtgacga
     5701 ggactgaaca atggccacta tctcttcaac ttcaattcct tccatacaaa cacagagtag
     5761 taacagggca tcacaaggta gcgatgttgc ttcacaaatc gcgcggataa gtcagcaaat
     5821 catcaaactc actcaacaaa ttaaagagat tgtagacact agcggcagtg ctgaagacaa
     5881 gcaaaaacag gccgaattga ttcaacagca aatcacccta ctggaaacac aactggcgca
     5941 gttgcagaaa cagcaggcag aaaaggcgca ggagaaagag cagcgtttgt ctctgaatgt
     6001 atcccttcta aatcctgttg aaaaaacgac tcatatcgat atctatatct gatacaacgg
     6061 atccccttcc cgaccagcca ttagcgcatt gccctggtct cgtaccacgt cccataacgc
     6121 ttcagcggcg gtggaaagtg aacggttctt cctgcgcacc agcatcaact ggcgttcaac
     6181 aaccggcgta atgcgcttta ccaccagtgg gctaccttca ggcaatggta gcgccagtgc
     6241 gggaagaata ctgatgccga tgcctgcggc taccatcggg aacagcgtcg ccggatgtcc
     6301 aatctcctgc acaatattcg cctgaatacc attgcgcgcc agcgctgcgt caatcagcgg
     6361 tcggctgcct gacgcgtaat cctgcaacac caatttcgct ccctgtaatg cctgccaggg
     6421 tacataatct tccacggcca aagcgctatc gcgatggcaa agcagaaaga aaggctcgga
     6481 aagaatcgct tcacattgca gatcgcccac tgggcctgga tcgatgacga tgccaaagtc
     6541 gacatctccc tggcgaatac tttccatcac ccactgctgc ggacgatcgt gcaggacaaa
     6601 ctgaatatct ggatagcggc gatggctttc agcaatgcat tgcggaataa gatgcgcgga
     6661 aatggtctgg ctggcagcga cccgaacttt tccgctcagt tgttgcccca tacgcccggt
     6721 atcgcgcaac gtgctgttaa gttcatccag cagtcgctca agacgcaaag ccaactgctg
     6781 ccctgcatct gtaagcacca cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg
     6841 attttccagc tccttcacac tgtgacttac tgccgactgg ctcaagccga tacgctctcc
     6901 tgcacgacta aaacttttct cctgcgctac tgtgacgaaa acctttaatt gttttaaaga
     6961 ataattcatc tgttttcttc atgaatggat gcaataaatc aattttattt ctcaaactga
     7021 aagaagcaca atagaaccat cgatcatctg gagtctttat gaaacttttt cgtatcctcg
     7081 atcctttcac cttaaccctg attacggtgg tgttgctggc ctctttcttt ccggccagag
     7141 gcgatttcgt ccccttcttt gaaaatctga ccaccgcagc tattgccctg ctgttcttta
     7201 tgcacggcgc gaagttgtcg cgtgaggcga ttattgctgg cggtggtcac tggcgactgc
     7261 atttgtgggt aatgtgcagc accttcgtgc tgtttccgat tctgggtgta ctgtttgcct
     7321 ggtggaaacc ggtaaatgtc gacccgatgc tctactccgg ttttctctac ttgtgcactc
     7381 tcccggctac cgtgcagtct gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg
     7441 cggcggtttg ttctgcgtca gcatccagcc tgctggggat tttcctttca ccattgctgg
     7501 ttggtctggt gatgaatgtt cacggtgcag ggggcagcct tgagcaggtc ggtaaaatta
     7561 tgctgcaact gctgctgccg tttgtgttgg ggcatctttc ccggccgtgg attggtgact
     7621 gggtgtcgcg caataaaaaa tggattgcga aaactgacca gacgtccatt ctgttggtgg
     7681 tttatacagc gttcagcgaa gccgtcgtta atggtatctg gcataaagtt ggctggggat
     7741 cattgctgtt tatcgtggtg gtcagctgcg ttcttctggc tatcgtgatt gtagttaacg
     7801 tctttatggc acgccgactg ggcttcaata aggcagatga aattactatc gtcttttgtg
     7861 gttcgaaaaa gagtctggca aatggcatcc cgatggcaaa cattctgttc cccacatcgg
     7921 tgatcggtat gatggtgctg cccctgatga ttttccatca gatccaattg atggtctgtg
     7981 cggtgctggc gcgtcgatac aaacgccaga ccgaacagtt acaggcgcag caggaaagca
     8041 gcgccgataa agcttaaagc ggacgcttca ggggctggac cagttgcgtc agcccctcgg
     8101 ttttaatcag cagcgtgatt tgcattagct caccgagctt ccctgccggg aactcatctt
     8161 tgcgggcaaa ccacagcaga tactcctccg gcaggtcgat taagcgacgc cctttgtatt
     8221 tgccaaacgg cattatcgta ttggccattt caatcagctg ctctttttcc atctcagcta
     8281 cccagcaaac gcagcatttc cgcttcgtcg atgacttcaa tgcccagttc ctgcgccttc
     8341 gccagtttag atcctgcagc ttcacccgca atcaccagat cggttttctt cgacacgctg
     8401 cccgcgactt tcgcgcccag ttcgaccagt cgagctttag cgtcatcacg cgacatctgg
     8461 cttaagctgc ccgtaagcac cacggtttta ccagcaaacg ggctgtcaat ctcttccgcg
     8521 ttgataacga tcggcgcagg ccagtgaaca ccttccgcca acagctcgct gatgacattg
     8581 cggttgcttt cttcggcaaa gaagttgtga acgtgggatg caacgacaat gccaacatca
     8641 ggcacctttt gcagctcttc aatcgaagcg gcttccagcg ctttcagcgt gccgaaatat
     8701 gccgccagac ctgctgcggt ggcctcgccg acttcacgga tgccaagtgc atagaggaag
     8761 cgagcaaagg tggtttcttt cgctttttcc agcgcgttaa ccacgttttg tgccgatttt
     8821 ggccccatac gctccagtcc ggtcagtttg cctgcggtga gtttgaacag atctgccgga
     8881 gtgtggacat attctttttc aaccagctga tcgatgattt tgtcgcccat tccgtcaaca
     8941 tccatcgcac ggcgggaaac aaagtgtttc agcgactctt tacgctgcgc accgcaaatc
     9001 aggccaccgg tacagcgggc aaccgcttca ccttccacac gctcaacgtc agaaccacat
     9061 accggacaat gcgtcgggaa tacaacctca cgggtatctt ccgggcgttc agaaagcacg
     9121 acgttaacca cctgcgggat cacgtcgcca gcgcggcgaa tcaccacttt atcgccaatg
     9181 cgtaaaccaa gacgttcgat ttcatccgca ttgtgtaagg ttgcgttact caccagcacg
     9241 ccagcaacat ggacaggttc cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc
     9301 tgaaactcga cgtcacgcac aaaggtcatc tgctcctgcg ccgggaattt aaacgctacc
     9361 gcccagcgcg gggcacgcgc gacaaagcca agctgctcct gctgttccag tgagttgacc
     9421 ttaatcacca cgccgtcgat atcaaagccc agcgtcgggc ggtcttcttc cactttgtga
     9481 tagaacgcca gcacttcttc cgccgattca caaagcgtta cccgatcgct gaccggcaac
     9541 ccccactttt taaattgcag taaacggcca agatgagtat ccggcagctc gccaccttcc
     9601 agaacaccaa cgccatagca gaaaaaagtg agcggtcgct tcgctgtaat acgcggatca
     9661 agctgacgca gtgaaccagc tgccgcatta cgtgggttag caaaaacttt cccgcccgtg
     9721 cgtcgcgcat cttcgttaat cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc
     9781 acttccagac gcgccgggat attctctcca tgcagcttca gcggaatggc gcgaatagta
     9841 cgcacattag acgtgatatc ttccccggtg gtgccatcgc cacgggtcgc ggcactgact
     9901 aaaacgccat tttcatacag aatactgacg gcaaggccat ccagcttcag ctcacagcac
     9961 caggtgactt tctcgttgtt tttcagacgg tcctgcacac gtttgttgaa agcaagaaag
    10021 ctttcttcat caaaaacgtt atccagtgac agcattggta cttcatggcg tatctggctg
    10081 aaagccgcca gcggcgcagc acctacacgt tgggtaggcg aatcaggcgt aatcagttct
    10141 ggatgtttag tttccagctc gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga
    10201 atttccggcg catccatcac atgataaaga tattcatgat ggcgaagcgt cgttcgcagt
    10261 tctgtcagtt gttgttcgat tgattccata tcgcaccatc aatgctaaaa acccccgaca
    10321 agcgggggtt cgaagaggag ttaatttgcc ttaagtgtat caggcgttgg cgtctttgac
    10381 ttcgcggatg atgtcctggt actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc
    10441 gtcaagcacg acaccgccca cttcatcggc aatatgctgc gcagattgca gcatcagctt
    10501 gaagttctgc agctcgtcac cgtaagacgg tacctgcata aagatagtga cacccggagt
    10561 agtgaaatcc ttcatttcag gatcaaaggt tcccggtttc accatattcg ccaggctgaa
    10621 taacgccggg ccgctgccat ccgggctaag atgacgatgg taaatattca tatcgccaaa
    10681 aatgaagccc gcttgttgaa tgctgttaag aagcagttca ccgtttagct cgctaccgtg
    10741 atgcgccgcg acgttcatga taatcaccgc ttctttgcgc ttcggtttat ccataactgg
    10801 agccggttcc gctacaggct caggctgtgg tgccgctacg ggttctgcag gctggaaagc
    10861 ctgttgtgcc ggttgcggtg ctgaatgcac aggctgcggc gctggcgcga cctgtggcga
    10921 aactggctgc tgcaacggct gttcaggctg cggctgatag gcaggctgct gcaccggctg
    10981 cgctggacgc ggagcatgtt gcggcggtac ctgcgcttca ggcggctgct ggaccggttg
    11041 acgcggctgc gcagacgcat aaggcggttg gtactggtgt tgcggcgacg gacgagcagc
    11101 ctcatgctcc tgagcgttag ccggggcatg attcacgcgg tgaacacgaa cctcaccaac
    11161 gccctcatca tcttcgacat cctcgtcata ataatcgtcg tcacgttttg acttcattcg
    11221 ttttaatggc cgatcgcgga acatagaaga tcgttcttta cggctggtcc agaaaccatg
    11281 taccagtaaa gcgattatgg cgatcgcgcc aacaatgatt aatatcagac gcaaatcctg
    11341 catcattata ttctctgttg ttctaacacc ttgccaccac ggcaaacatt tactcactaa
    11401 gagtatttgc cgattacctc aagtgcaagt gcactattaa ctttcacagc acaaagatag
    11461 atgaaatcgt gctttttgct gttttttcga acatatccta actgtccatt gcgcaattac
    11521 ccggtaaaat acgcagaatt ttcctgggat tggtcaaaag gagctcatcc tgattatggt
    11581 ttcatcattc acatctgccc cacgcagcgg tttttactat tttgcgcaag gctggaagct
    11641 cgtctcgcaa cctgggattc ggcgtttcgt tattttaccg ctgctggtca atattttgtt
    11701 gatggggggc gcattctggt ggctctttac acagctcgat gtctggatcc cgactctcat
    11761 gagttacgtt ccggactggc tacaatggct gagttatctg ttgtggcctc tggctgtcat
    11821 ctctgtgctg ttagtgtttg gctatttctt ctccacgatt gctaactgga ttgccgctcc
    11881 gtttaacggt ttattggctg aacaactgga agcacgattg actggcgcta caccgccaga
    11941 taccgggatt ttcggtatca tgaaagatgt gccgcgaatc atgaaacgcg aatggcaaaa
    12001 atttgcctgg tatctgccgc gcgcaattgt attactaatt ctttacttca tccctggtat
    12061 tgggcaaacc gtcgcgccgg tactgtggtt cctgtttagc gcctggatgt tagccatcca
    12121 gtattgcgat taccccttcg ataaccacaa agtgccgttt aaagagatgc gcaccgccct
    12181 gcgcacacgc aaaatcacca atatgcagtt tggtgcttta accagcctgt ttacgatgat
    12241 cccgctgctt aatctgttca tcatgcccgt tgccgtttgt ggcgcgacgg cgatgtgggt
    12301 cgattgctat cgcgataaac acgcgatgtg gcggtaacaa tctaccggtt attttgtaaa
    12361 ccgtttgtgt gaaacagggg tggcttatgc cgccccttat tccatcttgc atgtcattat
    12421 ttcccttctg tatatagata tgctaaatcc ttacttccgc atattctctg agcgggtatg
    12481 ctacctgttg tatcccaatt tcatacagtt aaggacaggc catgagtaag atttttgaag
    12541 ataactcgct gactatcggt cacacgccgc tggttcgcct gaatcgcatc ggtaacggac
    12601 gcattctggc gaaggtggaa tctcgtaacc ccagcttcag cgttaagtgc cgtatcggtg
    12661 ccaacatgat ttgggatgcc gaaaagcgcg gcgtgctgaa accaggcgtt gaactggttg
    12721 aaccgaccag cggtaatacc gggattgcac tggcctatgt agctgccgct cgcggttaca
    12781 aactcaccct gaccatgcca gaaaccatga gtattgaacg ccgcaagctg ctgaaagcgt
    12841 taggtgcaaa cctggtgctg acggaaggtg ctaaaggcat gaaaggcgca atccaaaaag
    12901 cagaagaaat tgtcgccagc aatccagaga aatacctgct gctgcaacaa ttcagcaatc
    12961 cggcaaaccc tgaaattcac gaaaaaacca ccggtccgga gatctgggaa gataccgacg
    13021 gtcaggttga tgtatttatt gctggcgttg ggactggcgg tacgctgact ggcgtcagcc
    13081 gctacattaa aggcaccaaa ggcaagaccg atcttatctc tgtcgccgtt gagccaaccg
    13141 attctccagt tatcgcccag gcgctggcag gtgaagagat taaacctggc ccgcataaaa
    13201 ttcagggtat tggcgctggt tttatcccgg ctaacctcga tctcaagctg gtcgataaag
    13261 tcattggcat caccaatgaa gaagccattt ctaccgcccg tcgtctaatg gaagaagaag
    13321 gtattcttgc aggtatctct tctggagcag ctgttgccgc ggcgttgaaa ctacaagaag
    13381 atgaaagctt taccaacaag aatattgtgg ttattctacc atcatcgggt gagcgttatt
    13441 taagcaccgc attgtttgcc gatctcttca ctgagaaaga attgcaacag taatgccagc
    13501 ttgttaaaaa tgcgtaaaaa agcacctttt taggtgcttt tttgtggcct gcttcaaact
    13561 ttcgcccctc ctggcattga ttcagcctgt cggaactggt atttaaccag actaattatt
    13621 ttgatccgcg aaattaatcg ttacaggaaa agccaaagct gaatcgattt tatgatttgg
    13681 ttcaattctt cctttagcgg cataatgttt aatgacgtac gaaacgtcag cggtcaacac
    13741 ccgccagcaa tggactgtat tgcgctcttc gtgcgtcgcg tctattaaaa actggcgcta
    13801 acaatacagg ctaaagtcga accgccaggc tagactttag ttccacaaca ctaaacctat
    13861 aagttgggga aatacaatgt tccagcaaga agttaccatt acagctccga acggtctgca
    13921 cacccgccct gctgcccagt ttgtaaaaga agctaagggc ttcacttctg aaattactgt
    13981 gacttccaac ggcaaaagcg ccagcgcgaa aagcctgttt aaactgcaga ctctgggcct
    14041 gactcaaggt accgttgtga ctatctccgc agaaggcgaa gacgagcaaa aagcggttga
    14101 acatctggtt aaactgatgg cggaactcga gtaatttccc gggttctttt aaaaatcagt
    14161 cacaagtaag gtagggttat gatttcaggc attttagcat ccccgggtat cgctttcggt
    14221 aaagctctgc ttctgaaaga agacgaaatt gtcattgacc ggaaaaaaat ttctgccgac
    14281 caggttgatc aggaagttga acgttttctg agcggtcgtg ccaaggcatc agcccagctg
    14341 gaaacgatca aaacgaaagc tggtgaaacg ttcggtgaag aaaaagaagc catctttgaa
    14401 gggcatatta tgctgctcga agatgaggag ctggagcagg aaatcatagc cctgattaaa
    14461 gataaacaca tgacagctga cgcagctgct catgaagtta tcgaaggtca ggcttctgcc
    14521 ctggaagagc tggatgatga atacctgaaa gaacgtgcgg ctgacgtacg tgatatcggt
    14581 aagcgcctgc tgcgcaacat cctgggcctg aagattatcg acctgagcgc cattcaggat
    14641 gaagtcattc tggttgccgc tgacctgacg ccgtccgaaa ccgcacagct gaacctgaag
    14701 aaggtgctgg gtttcatcac cgacgcgggt ggccgtactt cccacacctc tatcatggcg
    14761 cgttctctgg aactgcctgc tatcgtgggt accggtagcg tcacctctca ggtgaaaaat
    14821 gacgactatc tgattctgga tgccgtaaat aatcaggttt acgtcaatcc aaccaacgaa
    14881 gttattgata aaatgcgcgc tgttcaggag caagtggctt ctgaaaaagc agagcttgct
    14941 aaactgaaag atctgccagc tattacgctg gacggtcacc aggtagaagt atgcgctaac
    15001 attggtacgg ttcgtgacgt tgaaggtgca gagcgtaacg gcgctgaagg cgttggtctg
    15061 tatcgtactg agttcctatt catggaccgc gacgcgctgc ccactgaaga agaacagttt
    15121 gctgcttaca aagcagtggc tgaagcgtgt ggctcgcagg cggttatcgt tcgtaccatg
    15181 gacatcggcg gcgacaaaga gctgccatac atgaacttcc cgaaagaaga gaacccgttc
    15241 ctcggctggc gcgctatccg tatcgcgatg gatcgtaaag agatcctgcg cgatcagctc
    15301 cgcgctatcc tgcgtgcctc ggctttcggt aaattgcgca ttatgttccc gatgatcatc
    15361 tctgttgaag aagtgcgtgc actgcgcaaa gagatcgaaa tctacaaaca ggaactgcgc
    15421 gacgaaggta aagcgtttga cgagtcaatt gaaatcggcg taatggtgga aacaccggct
    15481 gccgcaacaa ttgcacgtca tttagccaaa gaagttgatt tctttagtat cggcaccaat
    15541 gatttaacgc agtacactct ggcagttgac cgtggtaatg atatgatttc acacctttac
    15601 cagccaatgt caccgtccgt gctgaacttg atcaagcaag ttattgatgc ttctcatgct
    15661 gaaggcaaat ggactggcat gtgtggtgag cttgctggcg atgaacgtgc tacacttctg
    15721 ttgctgggga tgggtctgga cgaattctct atgagcgcca tttctatccc gcgcattaag
    15781 aagattatcc gtaacacgaa cttcgaagat gcgaaggtgt tagcagagca ggctcttgct
    15841 caaccgacaa cggacgagtt aatgacgctg gttaacaagt tcattgaaga aaaaacaatc
    15901 tgctaatcca cgagatgcgg cccaatttac tgcttaggag aagatcatgg gtttgttcga
    15961 taaactgaaa tctctggttt ccgacgacaa gaaggatacc ggaactattg agatcattgc
    16021 tccgctctct ggcgagatcg tcaatatcga agacgtgccg gatgtcgttt ttgcggaaaa
    16081 aatcgttggt gatggtattg ctatcaaacc aacgggtaac aaaatggtcg cgcctgtaga
    16141 cggtaccatt ggtaaaatct ttgaaaccaa tcacgcgttc tctatcgaat ctgatagcgg
    16201 cgttgaactg ttcgtccact tcggtatcga caccgttgaa ctgaaaggcg aaggcttcaa
    16261 gcgtattgca gaagaaggtc agcgcgtgaa agttggcgat actgtcattg aatttgatct
    16321 gccgctgctg gaagagaaag ccaagtctac cctgactccg gttgttatct ccaacatgga
    16381 cgaaatcaaa gaactgatca aactgcccgg tagcgtaacc gtgggtgaaa ccccggttat
    16441 ccgcatcaag aagtaattct tgccgcagtg aaaaatggcg cccatcggcg ccatttttta
    16501 tgcttccgcc agcggcggca aaatcaattc atcgctctca tgctgctggg tgtagcgcat
    16561 cacttccagt acgcgcaacc ccgcacggtg caccgcatcg tttaacgcct tccctttcag
    16621 caaaccactg ataagctgag cacaaaacag gtcgccagtc cctttcaggt cggtttttac
    16681 ccgtgaatgg gaaatgacat tcacgctgtc ggcactgacc accaccacct gcatctcctg
    16741 attttcttca ttaccggagg cgctggtaat caccacccat tttaatgtgt ctgaaagcag
    16801 actttttgcg gcagcaatgg cactgtcgag atcgcggcaa tttttaccgg tcaggatttc
    16861 cagctcaaag atattggggg taattccctg cgccagcggc agtaaatatt gtcgatacgc
    16921 ctcgggaagg tcaggtttga cataaattcc gctatcaata tcgccaatca ccggatcgac
    16981 catgatcaat aggtcaggat ggtctttgcg tagcgcagtc agccactcgg caaggatttt
    17041 gatttgcgat gccgttccca tatagcccgt ggttacagcg cgcagctggc gcagcgcatc
    17101 acgctcctga agcgcacgca aataaccgct aaaccattca tccggaatcg caccaccgta
    17161 gaaagtgtca taatgcggcg tattgctcag caataccgtc ggcacggcaa aaacattcag
    17221 accgttctgt ttgatagcag gcacggcaat gctgttgccc acgctgccgt aaaccacctg
    17281 cgactgcacg gcgacgatat ccgcctgcag cgccctgctc ttatcgttaa acaacaacaa
    17341 actactcatt taattttttc tccttgccga tgatcctcat cgtattccaa ccgaaacttt
    17401 acctgattct ggcagtcaaa tcggctatta caaaacaagg ataaggtaat tcaatgaaga
    17461 aaatcatttg tctggtcatt acactactaa tgacactccc cgcttacgcg aagttaactg
    17521 cccatgaaga agcccgcatc aacgccatgc tggagggatt atcacagaaa aaggatttga
    17581 tatttgtgcg caacggtgat gaacatacct gcgatgaagc ggtttctcat ctgcgtctga
    17641 agctcggcaa tacccgtaac cgcattgaca ctgccgagca gtttattgat aaggttgctt
    17701 cgtcgtcatc gattaccggg aagccgtata ttgtgaagat ccccggtaag agcgatgaga
    17761 acgcacagcc ttttttacat gcgctaattg cgcagacgga taaaacggtg cctgcgcaat
    17821 aatcttaaat ccccgccccc tggctaaaat gctcttcccc aaacactccg gtggagaggt
    17881 agcgatcgcc acgatcgcag atgatcgcta ccaccaccgc accagggtta gcttttgcca
    17941 cccgcagtgc tccagcaacc gcgccgcctg agctgacgcc acagaatatt ccttcccgca
    18001 ccgccagttc gcgcatggtg ttttccgcat cgcgctgatg aatatccagc acctcatcca
    18061 ccagagaagc gttgaaaatc cccggcagat attccgcagg ccagcggcga atgccgggaa
    18121 tactgctgcc ttcttccggc tgcaggccga caatggtcac cggtttggat tgttcgcgca
    18181 taaagcgtga aacgccggtg atagtgccgg ttgttcccat gctggagaca aaatgggtaa
    18241 tgcgcccgcc ggtttgctgc cagatttccg gcccggtagt ggtgtaatgg gcgtaagggt
    18301 tatcgggatt attgaactga tcgagcagct ttccttcgcc acgattcgcc atctccagcg
    18361 ccagatcgcg cgcaccttcc atgccctgct ctttggtgac aagaatcagt tccgcaccat
    18421 aagcacgcat cgccgcacgg cgttcctggc tcatgttgtc gggcatcagc aatttcatgc
    18481 gatagccttt aagcgcggca atcattgcca gcgcaatgcc ggtgttacca ctggtggctt
    18541 cgattaacac atcacccggt ttaatttccc cgcgcttttc cgcctcgacg atcatcgaaa
    18601 gtaccgcacg atctttcacc gaacctgccg ggttattgcc ttccagtttc aaccagactt
    18661 cactgccgtt attcggcccc attcgctgca actttaccag aggcgtattg cctattgttt
    18721 gttctaatgt actcacgatc tctatccata cgtggtgttg cctgatgcga cgcttgcgcg
    18781 tcttatcagg tctacaggtt acaaaccttg ccattaaaaa gcccggatcg cgggaagcgc
    18841 ctccgggcgt ttaacattca ctcaacctat caggcgcttt gtgcgagagc aagttcctca
    18901 tctcgggttt cgatacgctc gtcgccgtta tacagccgcg catgttgcag accaacgaat
    18961 aaacgctcgc cacgctgcgg ggcatcgtcg ccatgcatca cgaccgtcag cggttcgttg
    19021 taccacccca gcggctgcac cactaattgg gtgtagtgac ctttcgggct ggcttccagt
    19081 acctgtaccg gcagcggcga atcgagactg gtacggcggc tgatatccac ttcccacggg
    19141 cgcaggaaga gatccaccgg cccctgatac gcaggcgtat accccagcgg ccagcgatgt
    19201 gcgccaacat ggaactgccc gccgcgaatg gttccctgca ggcggttcac ttcgcccata
    19261 aattcgagca caaatcgggt cgctggttcg cgccatacct ggtccggcgc atcagcctgc
    19321 tcgatattgc cctggctcat caccactaca cgatcagcta cttcggtcgc ttcttcctgg
    19381 tcgtgggtca cgaacacgct ggtgaatttc agctcttcat gcagttgacg cagccagcga
    19441 cgcagctctt tacgcacctg cgcatccagc gcgccaaacg gttcatcaag caggagaatt
    19501 tgcggttcaa cagccagcgc gcgcgccagc gccacgcgct gtttctggcc gccggaaagc
    19561 tgtgccggat aacgatccgc cagatgcgca agctggacca tctccagcaa ttttgtcact
    19621 ttcgctttga tggctgcggc attcgggcgc tcgcgacgtg gcagcaccgt caggccaaaa
    19681 gcgatattgt cgaacaccgt catatgacgg aacagcgcgt aatgctggaa cacgaaaccg
    19741 actttacgat cgcgtgcgtg caggcggctc acgtcggtac cgtggaagcg aatatgcccg
    19801 ctggtttgat gctccagccc ggcaataatg cgcagcagcg tggtttttcc ggaaccggac
    19861 ggccccagca gcgcgaccat ctgaccagaa ggaatatcca gtgagatatc gttcagcacc
    19921 tgggtgcgac caaacgactt cttaatattg gcaatctcaa tgctcatgat gctcctcctg
    19981 ctgcgcgcgt ttttcctgat tctccagacg ccactgcaac atactcttta aaaacagggt
    20041 gataatcgcc atcagcgtca acagcgcggc ggcggtaaag gagccgacgg tgttgtagtc
    20101 ctgctccagc aattcaatct gtaacggcag cgaaagtgtt tcgccgcgaa tcgagccgga
    20161 aaccaccgac accgcaccaa actcgccaat cgcgcgggcg ttggttagca ccacaccata
    20221 cagcagcgcc cagcgaatgt tcggcaatgt gacgcgacgg aacatctgcc agccagacgc
    20281 gccaagcaaa atcgccgctt cgtcttcctg gctgccctgg cttaacatca ccggcaccag
    20341 ctcgcgcacc acaaaaggac aggtcacaaa gatggtgacc agcaccattc ccggccagga
    20401 gaacataatc tgcaaattat gttcatccag ccagccgccc agcggaccgt tagagccgta
    20461 gaacagcaga tacaccagac cggcaaccac cggcgatacg gcaaacggaa tgtccagtag
    20521 cgtcagcagt aactggcgtc cggggaagtt aaagcgcgtt accagccacg ccagcagaat
    20581 gccgaacacc aggttcaccg gtacggcaat cagcgcaatc atcaccgtca gccaaatggc
    20641 gtgcagcatg tccggatcgg ccagattctg taaaaccggc atcagccctt tgctgaacgc
    20701 ctgtacgaag atataaatca tcggcaccag caggatgaac gccgatacca gcatcccgat
    20761 gccaatcaga aaccatttgc cccagttaat cgggcgcgcg tcataacgct tcaattgggt
    20821 aacttccgcc attaatgacc taccacacgc cgaccaaagc gactttgcag agtgttaatt
    20881 gagaacagca gcagcaggga taccgcgagg atcaccgaag caatcgcgct cgccgccggg
    20941 taatcaaact cctgcaagcg cacaaaaatc atcagcgacg tcacttccgt cttccaggcg
    21001 atgttcccgg cgataaatat caccgcgcca aactcaccaa gactacgggt aaacgacaac
    21061 gccacgcccg ccaccagcgc tggagaaagt tccggcagca ccactttgca gaaactctgc
    21121 cagcgcgttg caccaagcgt ttccgccgct tcttcatatt ctgggcctaa ctcttccagc
    21181 accggctgca cggtacgcac cacaaacgga atgctggtaa aggccatcgc caccgcaata
    21241 ccaagccagg tataggtgac tttgatatca aacttcgcca gccattcacc gtaaaaaccg
    21301 tttacggaaa agagcgaggc cagcgttaaa ccggcgactg ccgttggcag cgcaaagggt
    21361 aaatccatca gcgcatcaag cagcgtgcgg cccggaaagc gataacgtgt taaaatccac
    21421 gccatcagca gaccgaaaac gccgttaaaa atcgatgcca caaacgccga cagcagcgtc
    21481 actttgtagg ccgcgaccac ttgtgggttg gtgatcacct cccagtactg cgcccagctc
    21541 atctgagcca gctgcatcac cagcgcggag agcggcagca gcaaaatcag gcacacaaac
    21601 agcagactgg tgccgaggct taaggtaaag cccggcagca cgcgtcttga tgaaactgca
    21661 aacatcagtt acgccccgcc gctaacagct tgtctaactc gccgccgctg gtgaagtggg
    21721 ttttcatcac ttccggccag gagccaaatt tgtcttccac gcggaacagc tcggtctgcg
    21781 ggaatttatc tttcagtttg tccatgactt ccgggttatt tacgcggtag taatagtcgg
    21841 tgatgatggt ctgcgcctgc gggctgtaga gccagttcag gtaggctttt gctgcttttt
    21901 ccgtaccgtt ggcctgcacg tttttatcga cccacgccac cgggaattcc gccagaatgt
    21961 tggttttcgg aatcaccact tcaaagcctt gcgcttcata ctgtttacgg atgttgttca
    22021 cttccgactc gaagctgatc agtacatcgc ccaggccgcg ctcggcaaaa gtggtggtcg
    22081 cgccacgacc gccagtatcg aacacttcaa cgtttttcag gaactgggtc ataaactgtt
    22141 cggttttggc tttgtcgcca ccgtcagctt tatccgctgc accccatgcc gccagatagg
    22201 tataacgcgc gttacccgac gttttcgggt tcgggaaaat cagcttcacg tcggagcgca
    22261 ccaggtcgtt ccagtcgtgg atattcttcg ggttgccctt acgcaccagg aagcccatgg
    22321 tggagtagaa cggcgagcta ttattcggca ggcgcgactg ccagtcagcc gggatcagct
    22381 tgcctttgtc gtgcagaatt tgtacgtcgg tcacctggtt ataagtgaca acgtcggttt
    22441 ttaagccctg caaaattgcc agcgcctgtt ttgatgaccc ggcatgagat tgttttatcg
    22501 tcagtttgtc gccgccgtta tcttttgccc attgctgttc aaacggcgga ttcagggcgg
    22561 caaacagctc gcgggagacg tcataagaac tgttcagcag ttccgttgcc tgtacatggc
    22621 ccgccagcag cagagaagcg accagcgcga gtgagttctt tttcagtaag ttaacggcca
    22681 ttgcgcaccc ttataaattt aatgactttc taatagccat catatttata acggacgtga
    22741 aaggagtaac ggttttatat accgtttggt gatttggaag ttaaaaaggg aataagaact
    22801 tggttcaaca ggcgtaattg ttgcaggcag tttggacacg gacagcgcgg agaaaccgga
    22861 gcgtacacat agtacgtgag tatttcgagc actgcccggg gccaaaatga caaataaaat
    22921 agcctggtga acttagttca agacgaaatc ctccccacac atgcagggag gaaacagagg
    22981 tgaatcagat accgacgcta accgtctccg gcagtgtgct gccgccatca atcacattct
    23041 gtgtaccggt taaatagctg gattcatccg atgcgaggaa ggccgccagt tcgccgactt
    23101 ccagcggatc ggcgaggcga cgcatcggga ttgctttcgc catttcagtc agcaccgact
    23161 ctggatcttc caggttcgac tggcgggcaa tgctttccgc cattggtgtg cgcacgtatc
    23221 ccgggcaaat ggcgttaacg cgaataccag actgcgcgta ctccaccgcc agcgattttg
    23281 tcaggccaac aatcgccgct ttcgttaagg cgtacgccgt ttcgccagga tcggccacca
    23341 tatcaccagt gactgaagac atcatcacaa tgcgaccatc tttgcgcgca atcatctccg
    23401 gcagcaccgc cttcgtgacg ttccatacgc ctttaatatt gatatcaata tggaaatcgc
    23461 gatcgtcatc gctcatatcg aggaaactgc ccagacgaca aacgcctgcg ttattcacca
    23521 ggatatcaat gcgcccttct ttttccttcg cgcgtttgat agctgcggct accgacgccg
    23581 ggtcacgcac atcggcgaca accgccgtac agcgatgacc acgaccacac agttcgtccg
    23641 ccagcttttc gatctcaggg gagatatcca gcaagattag gttcgcgcca tgacgtgcaa
    23701 aagttctggc aattccttcg ccaattccct gcaatgcgcc cgtaatcagt gctgtcttgc
    23761 ccgtgagttt acccatttta atgctctcct tgttaataca gagcgttaca ctccattccc
    23821 tctgaaaata caggataaac aaaatattgc tgcggatcag ctcactaatt caggaaaatc
    23881 tcaatccagt cgctgcttta tgcatcattt caggcgttgt gttagctcac tgctgcgctg
    23941 aatcattttc aatgacgcca catcattgag ttgcacaaga ccaacaaaca gtaaatcggt
    24001 aacagaattt tgcgccgtgc gggtagacat cgacgagcta cgccattcgg tttcaccgga
    24061 aacggtatcg agcgtgaaat gcgccagccg ccgcagcgga gagtcagcca gagaagtaat
    24121 ggcaatcacc gttgccccct gctttcgtgc cgcttctgcg cacaagacaa tctctttttt
    24181 actgccgctg taagagatag cgatttgtac atcgcctttt ttcaacgcct gggaaacggt
    24241 ggcttgtacg tgcgtgtcgg cttcgcaggc cacgcgatag ccaattttca tcagtttgaa
    24301 cgacagatcg cgccccacca gcgccgatcc gcccaggccc gttatctgga taaacggtgc
    24361 tttactgatg acctcaatga ttttttgcag ccgcgcgtag tcgaacaacg cacaggtttg
    24421 ctccagcgcc agctcttttt cgcgattcaa tttgcgggca atcacttcca gcgaatcatc
    24481 actggtaatc gaactgtgca ggtgcagcgc cgtggtattc gttttctccc ggctggcact
    24541 gtattcgcct attaacgcca tacgcaattc agtaaatcct tgcgcgccga gtttttgggc
    24601 aaatttcact atgctcgact ggctaatgcc gagctgtttc gccatctgcc gtgaagaaac
    24661 ggattgcagc tcactcacgt tggcctgcag aaagtcagcg attttttgct cgttttcggt
    24721 gaattcactc cccgcattgc ttattttcgt caggtacaac atcttccact ctcctgggct
    24781 ggcagcataa attccgctta agttcttatg acgctcttca cactctgcga gtgtagactc
    24841 aatgattcct ttaagtctgt caattcagga tatatgattc catacatctg caattatttg
    24901 atcgtaaata gtaaggtcac caccgatgca acttgaaaag atgattactg aaggctcgaa
    24961 cgccgcctcg gctgaaattg accgcgtatc aacgctggaa atgtgccgga ttatcaacga
    25021 tgaagataaa accgtaccgc ttgccgttga gcgcgtactg ccggatatcg ccgcggcgat
    25081 cgatgttatc cacgcccagg taagcggcgg cgggcgtctg atttacctcg gtgcgggaac
    25141 atccggtcgt ctggggattc tggatgccag cgaatgtccg cccacctacg gcgtgaaacc
    25201 gggtctggtg gttggtttga ttgctggcgg cgaatatgcc attcagcacg cggtggaagg
    25261 cgcggaagat agccgggaag gcggtgttaa tgatctgaaa aatattaatt taacggcaca
    25321 ggatgtggtg gttggcattg ctgccagcgg tcgcacgccg tatgtgattg ccggactgga
    25381 atacgcacgc cagctcggct gccgcacagt gggaatttcc tgtaatccgg ggagcgccgt
    25441 ttcaaccacc gctgagtttg ccattacacc gattgtaggt gccgaagttg ttaccggttc
    25501 ttcgcggatg aaagcaggta cagcgcagaa actggtgctc aatatgcttt ccaccgggct
    25561 gatgattaaa tccggcaaag tgttcggcaa ccttatggtc gatgtggtcg ccaccaacga
    25621 aaaactgcat gtgcgccagg tcaatatcgt taaaaacgcc accggatgta atgccgaaca
    25681 agcggaagcg gcgttaattg cctgcgagcg caactgtaaa acggccattg tgatggtgct
    25741 gaaaaatctc gatgccgcag aagctaaaaa acgcctggat caacacggcg gctttattcg
    25801 tcaggtttta gacaaggaat aacccatggc caaagagatc agcagtgaac ttctgaacac
    25861 cattcttacc cgtgtcggcg gaccgggaaa tatcgccagt tgtggtaact gtatgacgcg
    25921 cctgcatctg ggtgtacatg acagttcact ggttgatccc aatatcaaaa cgctggaagg
    25981 cgtgaagagc gtcattttga ccagcgacca ggtgcaggtc gtttttggac ctggtaaagc
    26041 gcatcgtgcc gcaaaagcga tgagcgagct gctgggcgag gcaccggtac aggatgccgc
    26101 agaaatcgcc gcccagaaca aacgtcagtt aaaagccaaa caaacctccg gcgtgcaaca
    26161 atttctcgcc aaattcgcca ccatcttcac gccgctgatc cccggtttta ttgccgccgg
    26221 tctgctgctg gggatagcga cgttaattgc cacggtgatg cacgttccgg cagatgctca
    26281 gggcacgcta cccgacgcgc tgaattttat gaaggtgttc agcaaaggtt tgttcacctt
    26341 cctggtgatt ctggtgggct ataacgccgc ccaggcattt ggcggcacgg gcgtcaatgg
    26401 cgcaattatt gccgcactct ttttgctcgg ttataacccc gctgcaacca ccggttacta
    26461 cgccggtttt cacgatttct ttggtctgcc catcgatctg cgcggcaata ttatcggcgt
    26521 gctgattgcc gcgtgggcct gcgcccgcat tgaaggcatg gtgcgccgct ttatgccgga
    26581 cgatctcgac atgctgctga cctcgttaat caccctgctg atcaccgcta cgcttgcgta
    26641 cctgatcatt atgccgctgg gtggctggtt attcgaaggt atgtcatggc tgtttatgca
    26701 cctgaacagt aatccgttcg gttgtgcggt tttagccggg ctgttcctga tcgccgtggt
    26761 atttggcgtg catcagggct ttattcctgt ttacctcgcg ttaatggaca gccagggatt
    26821 caacagctta tttcccatcc tttcaatggc aggcgcgggc caggtgggcg cggcgctggc
    26881 actctactgg cggtcgcaac cgcacagtgc gctacgcagt caggtacgcg gggcgattat
    26941 tcccggcctg ctgggcgttg gtgaaccgct gatttacggt gtcaccctgc cccgcatgaa
    27001 gccgtttgtc accgcctgtt taggcggtgc ggcgggcggt ttgtttatcg gcctgatagc
    27061 ctagtggggt ctgccgatgg gcttaaacag cgcctttggg ccgtctggtc tggtagcgct
    27121 gccgctgatg acttccgcac aaggcatcct tccggccatg gcggtttatg ctggcgggat
    27181 tctggtggca tgggtttgcg ggtttatttt caccacgctc tttggctgcc gtaacgtcaa
    27241 tctggactga tatcatgaaa cggacaatgc tctatctttc tctgctggct gttagttgta
    27301 gcgtcagcgc cgcaaaatac cctgttctga cagaaagctc gccagagaaa gcagggttta
    27361 acgtcaaacg gcttaaccag atggatcgct ggattagcca gcaagttgat gccggttatc
    27421 ccggcgtaaa cctgctgatc attaaagata atcagattgt gtatcgcaaa gcctggggag
    27481 cggcgaaaaa gtacgatggc agcgtgttga tggaacagcc tgtcaaagcc accaccggga
    27541 cgctgtatga cctggcctca aacaccaaaa tgtacgccac gaacttcgcg ctgcaaaagc
    27601 tgatgagcga aggcaaactg catcctgacg atcggattgc gaaatatatt ccggggtttg
    27661 ccgatagtcc aaatgacacc attaaaggca aaaacaccct gcggat
//
LOCUS       DAJTNA010000035        27701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTNA010000035 DAJTNA010000000
VERSION     DAJTNA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27701
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.035"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..379
                     /locus_tag="OO161_001278"
     CDS             <1..379
                     /locus_tag="OO161_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5462416.1"
                     /translation="ICSNKHPRITDNSTYEQFMAEEFTQLISKSAGVDDIQMEIDEKF
                     MNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLYI
                     SYNKSSLNNLVFSRFIDRLNESF"
     gene            526..1746
                     /locus_tag="OO161_001279"
     CDS             526..1746
                     /locus_tag="OO161_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5462417.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQAPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1719..2348
                     /locus_tag="OO161_001280"
     CDS             1719..2348
                     /locus_tag="OO161_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR5462418.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2349..3498)
                     /gene="ybdL"
                     /locus_tag="OO161_001281"
                     /pseudo
     CDS             complement(2349..3498)
                     /gene="ybdL"
                     /locus_tag="OO161_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3607..4695
                     /gene="hcxA"
                     /locus_tag="OO161_001282"
     CDS             3607..4695
                     /gene="hcxA"
                     /locus_tag="OO161_001282"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5462419.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4705..4902)
                     /locus_tag="OO161_001283"
     CDS             complement(4705..4902)
                     /locus_tag="OO161_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5462420.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5084..7189)
                     /gene="cstA"
                     /locus_tag="OO161_001284"
     CDS             complement(5084..7189)
                     /gene="cstA"
                     /locus_tag="OO161_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5462421.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWTPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7370..7783)
                     /gene="entH"
                     /locus_tag="OO161_001285"
     CDS             complement(7370..7783)
                     /gene="entH"
                     /locus_tag="OO161_001285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5462422.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7786..8532)
                     /gene="entA"
                     /locus_tag="OO161_001286"
     CDS             complement(7786..8532)
                     /gene="entA"
                     /locus_tag="OO161_001286"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5462423.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8532..9389)
                     /gene="entB"
                     /locus_tag="OO161_001287"
     CDS             complement(8532..9389)
                     /gene="entB"
                     /locus_tag="OO161_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5462424.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYSKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9403..11013)
                     /gene="entE"
                     /locus_tag="OO161_001288"
     CDS             complement(9403..11013)
                     /gene="entE"
                     /locus_tag="OO161_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5462425.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQRLASRASA"
     gene            complement(11023..12198)
                     /gene="entC"
                     /locus_tag="OO161_001289"
     CDS             complement(11023..12198)
                     /gene="entC"
                     /locus_tag="OO161_001289"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR5462426.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHIPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12573..13529
                     /gene="fepB"
                     /locus_tag="OO161_001290"
     CDS             12573..13529
                     /gene="fepB"
                     /locus_tag="OO161_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5462427.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13533..14783)
                     /gene="entS"
                     /locus_tag="OO161_001291"
     CDS             complement(13533..14783)
                     /gene="entS"
                     /locus_tag="OO161_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5462428.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLVLFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14894..15898
                     /gene="fepD"
                     /locus_tag="OO161_001292"
     CDS             14894..15898
                     /gene="fepD"
                     /locus_tag="OO161_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5462429.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGCGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15895..16887
                     /gene="fepG"
                     /locus_tag="OO161_001293"
     CDS             15895..16887
                     /gene="fepG"
                     /locus_tag="OO161_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5462430.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16884..17699
                     /gene="fepC"
                     /locus_tag="OO161_001294"
     CDS             16884..17699
                     /gene="fepC"
                     /locus_tag="OO161_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5462431.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17696..18829)
                     /gene="fepE"
                     /locus_tag="OO161_001295"
     CDS             complement(17696..18829)
                     /gene="fepE"
                     /locus_tag="OO161_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5462432.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAY
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19044..22925)
                     /gene="entF"
                     /locus_tag="OO161_001296"
     CDS             complement(19044..22925)
                     /gene="entF"
                     /locus_tag="OO161_001296"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5462433.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCEVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22922..23140)
                     /gene="ybdZ"
                     /locus_tag="OO161_001297"
     CDS             complement(22922..23140)
                     /gene="ybdZ"
                     /locus_tag="OO161_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5462434.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(23143..24345)
                     /gene="fes"
                     /locus_tag="OO161_001298"
     CDS             complement(23143..24345)
                     /gene="fes"
                     /locus_tag="OO161_001298"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5462435.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24588..26828
                     /gene="fepA"
                     /locus_tag="OO161_001299"
     CDS             24588..26828
                     /gene="fepA"
                     /locus_tag="OO161_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5462436.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSRVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27003..27623
                     /gene="entD"
                     /locus_tag="OO161_001300"
     CDS             27003..27623
                     /gene="entD"
                     /locus_tag="OO161_001300"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5462437.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
BASE COUNT         6639 a         7720 c         7163 g         6179 t
ORIGIN      
        1 aatttgtagt aataagcatc cgcgaattac tgacaatagc acttatgaac aatttatggc
       61 agaagagttc acccaattaa tatccaaatc agcaggagtt gatgatattc aaatggaaat
      121 agacgagaag tttatgaatc gcaaaatttc atttcgtggt tcctctttgc tgacaataat
      181 taatagcatt gcagttacag atttactggg aatagtcccc tatgaacttt ataactccca
      241 ccgtgacttt cttaatttaa aagagataaa actagaacat ccgttacctt caataaagct
      301 ttacatttcc tataataaat cttctttaaa taatttggtg ttctcccgat ttattgaccg
      361 tctaaatgag agtttttgat atataactac agacagctac tataactttc atctatttat
      421 tcacagcgcg aatgaaaaat aaaacactct gctttacagt gtttacttta taattaacag
      481 tcttatctca ctgttcaccc tgtaacgttc agagagcgtc ttcagatgtc tatttataaa
      541 attcctcttc cgctcaatat tctggaagct gcacgagagc gtattacctg gacactcaac
      601 accctacccc gcgtttgtgt ctcattttct ggcggcaaag actctggttt aatgctgcat
      661 ctgacagccg aacttgcccg acaaatgggc aaaaaaatct gcgttttgtt tatcgactgg
      721 gaggcgcaat tctcttgcac tattaactat gttcagtccc tgcgcgagtt gtacaccgat
      781 gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc cctttcacaa
      841 taccaacccg aatggcagtg ctgggaacct gatgtcgaat gggtgcgtca ggccccgcaa
      901 gatgcgataa ccgatcctga ctttttctgc ttttaccagc caggcatgac cttcgaacaa
      961 tttgtacgtg agtttgccga atggttttca caaaaacgtc cggcggcgat gatgatcggc
     1021 atccgtgcgg atgagtccta caaccgtttt gtcgccatcg ccagtttaaa taaacaacgt
     1081 tttgccgacg ataaaccatg gactacggcc gcaccaggcg gtcatagctg gtacatttac
     1141 cccatttacg actggaaagt ggctgatatc tggacctggt atgcaaatca tcaaagtctc
     1201 tgcaacccac tgtataacct aatgtatcag gcaggcgttc ctctgcgtca tatgcgaatt
     1261 tgcgaacctt ttggcccgga gcaacgacaa ggattatggc tctatcacgt tatcgaaccg
     1321 gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg catttacgcc
     1381 ggacatgaca atcatttcta tgggcatcgg aaaatcctca agccagaaca tttagactgg
     1441 caagaatatg cattattgct gctcaatagc atgccggaaa aaacagctga gcattaccgc
     1501 aataaaatcg ccatttattt gcactggtat cagaaaaaag gcatcgaggt tccacaaacc
     1561 cagcaagggg acattggcgc gaaagatatc ccctcctggc ggcggatatg caaagtttta
     1621 ctcaataacg attactggtg tcgggcatta tcatttagtc ctacgaaagc gaagaactat
     1681 cagcgttata acgaacggat aaaaggaaaa cgtcaggaat gggggatact atgcaacaac
     1741 gattaacgca ggatttaacg caatttctcg ccagcttgcc ggaagatgat cgcatcaaag
     1801 cgattaatga aatccgcatg gcgatccatc aggttagccc ctttcgtgaa gagccagtgg
     1861 attgcgttct gtgggttaaa aacagccaac ttatgcctaa tgattacaac ccaaacaacg
     1921 tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga aatagatggt tttacccaac
     1981 ccattgtggt cacacatacg gataaaaatg ccatggaaat cgtggatggt tttcaccgcc
     2041 atgaaattgg taaaggctca tcatcattaa aattacgctt aaaaggttat ttgccggtga
     2101 cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac tattcgtcat aatcgcgctc
     2161 gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg tgaacttagc cagttgggat
     2221 gggacgataa taaaattggc aaagagctgg gaatggacag cgacgaagtg ctgcgcctga
     2281 agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca atattcccgc gcctggacgg
     2341 ttaaatagct aaagctggcg caggcgttca gctgctgcca gcaacgtcga ttccttcttg
     2401 gcaaaacaga gacgaatcag tttatgtggg aagggatcgg cgcaaaacac cgacagcgga
     2461 atcgccgcca cgccgtgctc ccgcgtcagc cactggcaaa actcaacatc atccagggta
     2521 gaaacctcgc tgtaatccac cagcaaaaag tatgtacctt cacacggtaa aatctccagc
     2581 cggctttcat ttaaagcatt caccagaata tcgcgcttct ggcgataaaa gtccggtaac
     2641 gcaagataat gctcaggttc tgcacgtagc atatcagcaa gcgccagctg tgccggggta
     2701 ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt cggcgctgat gggcgctggc
     2761 gcaacacaat aacccacttt ccagccggtc atatgataga tcttgccaaa tgaagaaacc
     2821 gccactgccc gctcacgcag ctgcggatgc gccagcacac tggcatggcc ctgttgtgaa
     2881 aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa tctcgtgccc ggcgatcgcc
     2941 tgccacaaag cggcgaaatc agcctgctgc cagacagttg cactggggtt atgcggagtg
     3001 ttgaggatca ccagtctggt gcgctcgctt aataatgcgg caaattcctg ccagtcaagg
     3061 tggttgcagt gccatacgct tcactattcc cccagaaagc gcgatggcgg gggcgtaact
     3121 gtcatagctg ggatcaaaac aaatcacttc atcgccattg cgcaccagtg cggtaatcgc
     3181 cgcgtataac gcttccgtcg cccctgccgt tacggtgata tcgctatcgg catctggttg
     3241 atagccatac aaacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt
     3301 catgggcgcg tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata
     3361 gcgcggacca tcaaaatcag gaaagccttg cgacaggtta atcgcctggt gttgctgcgc
     3421 cagcgcgctc atctgggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat
     3481 cagagggtta tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa
     3541 cacgatgcta gcatttggaa atcaagacgt ttagatgtct aaataaaaca ataaggacaa
     3601 cacaacatgc ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta cttttcacat
     3661 ccaagaagtt tcaatcacct gaacgatttt ttcactgatg aacaactttc tcgcgcggtg
     3721 tggatctacg gcaaacgcgc cattgctgcg gcgcaaacca aacttccgcc agcgtttgga
     3781 cttccagggg caaagcatat tttgtttcgt ggtcattgca gcgaaagcga tgtacagcaa
     3841 ctggcggctg agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg cggcgcactg
     3901 ctcgacaccg cgaaagccct cgcccgccgt ctcggtctgc catttgttgc cgttccgacg
     3961 atcgccgcta cctgcgccgc ctggacaccg ctctccgtct ggtataacga tgccggacag
     4021 gcgctgcatt atgagatttt cgacgacgcc aattttatgg tgctggtgga accggagatt
     4081 atcctcaatg caccgcaaca atatctgctg gcggggatcg gtgacacgct ggcgaaatgg
     4141 tatgaagcgg tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt gcggctgggg
     4201 atcaataatg cgcaggccat tcgcgacgtc ttgttaaaca gtagcgaaca ggcacttgcc
     4261 gatcagcaaa atcagcagtt aacgcaatca ttttgcgatg tggtggatgc tattattgct
     4321 ggtggtggga tggtcggtgg tctgggcgat cgttttacgc gtgtggcggc ggctcatgcc
     4381 gtgcataacg gtctgactgt cctgccgcaa accgagaagt ttcttcacgg caccaaagtc
     4441 gcttacggaa ttctggtgca aagcgccttg ctgggtcagg atgatgtgct ggcgcaatta
     4501 accggggcgt atcagcgttt tcatctgccg actacgctgg cggagctgga agtggatatc
     4561 aataatcagg cggagatcga caaagtgatt gcccacacct tgcgcccggt ggagtccatt
     4621 cattacctgc cagtcacgct gacaccagat acgttgcgtg cagcgttcga aaaagtggaa
     4681 tcgtttaaag cctgacgtgc aaatttagca gcagcgcgcg ccgccttttc caccgtagcg
     4741 cgcgtcctgc cgctcacgga aaaactcctc gtaggtcatc ggcgtttgat cgggatggtt
     4801 aacccgcata tgttcgacat agttgtcgta atcaggcata ccaatcatca gcttcgccgc
     4861 ctgacctaaa tattttccgg cttttgccag tgaatcaaac atcttggcct ccgctaacca
     4921 tgttgctgaa agagtgccgg atgcggcgtg aacgctttat ccggcctaca aatgctatca
     4981 acgcactcgg tcagtcccct cgccccatcg gggagagggt tagggtgagg gggaaccctc
     5041 ggcactatgc caacattcgc caacatcccc cctcactctg actttagtgc gcgcctttcg
     5101 cctgcgctac gatctcctcg acattttccg gcatcggctc atacggcgtt tctttcgccg
     5161 ttggcttcgg atctttcaat gccgcaagtg ccgtcttaat cgagaacagt gccagaacca
     5221 ccacgaccac cataaagaag atggttaacc cggcatccag acggttgttg aacaccagtt
     5281 gcgccagctg tgactcagta tactgcgacg gaatattgcc gctgtcgatc attgcctgga
     5341 acttgttagc aatggccagg aagccgactt tcgcatccgg gctaaacgct ttctgccagc
     5401 cagcggtcag ggtacaaatc agcagccagg ccgttggtac cagcgccacc caggcgtaac
     5461 gttgacgttt catcttgaac aacaccacgg cacagagcat cagcgccatc cctgccagca
     5521 tctggttggc aataccaaac agcggccaca gagtgttaat accgcccagc ggatcgacca
     5581 cgccctgatg gaggaagtag ccccacgcca gtacgcacag cgccgttgcc agcaggttag
     5641 caggcagtga atcggtccgc ttcaggccag gagacaccac gcccagcaga tcctgcaaca
     5701 taaagcgcgc agcacgcgta cccgcatcta ccgccgtcag aataaacaga gcttcaaaca
     5761 gaatggcgaa gtgataccag aacgccacat ccatcatacc acccagcgca ccgtgcagga
     5821 tgtaagccat ccccaccgcc aacgtcggcg caccgcccgc acgggaaatg atcgactgtt
     5881 cacccacttc gctggcaatc tggttgagcg tatctggtgt aatgctaaag ccccagctac
     5941 tcaccacctg cgcggcagaa gcgaccacat ccgccgtccc tgccggagcc agcaccgcca
     6001 ttgggctgtt catggcaaag taaacgcccg gatcgatgat acaggcggaa accagcgcca
     6061 taatcgccac gaaggattcc attaacattc caccgtagcc gataaaacaa gcctgccctt
     6121 cgttcgccag catcttcggc gtggtgccgg aagagatcag cgcatggaag ccagacaccg
     6181 cgccacaggc gatggtgata aacaggaacg ggaacaggtt accggtccat accgggccag
     6241 tgccatcgac aaatttggtc agcgcaggca tggtcagcgt cgggcgcata atcaaaatgc
     6301 ctaccgccag accgacgatc gtcccgattt tcaggaaggt agagaggtag tcacgcgggg
     6361 ccagcagcaa ccacaccggc agcaccgccg ccacaaaacc gtaacccacc agcatccagg
     6421 tcagctgcac gccggtaaag tcaaagtacg gtgtccaggt cggactttct gccacccagc
     6481 cgccagagat aatggcgaaa atcaggaata ccagaccgat gaccgacact tcaccaatac
     6541 gccccggacg cagatagcgc aggtagatcc ccataaacag cgccagcgga atggtgaacg
     6601 caacggtata cgttccccac gggctatgag ttagggcttt caccacgatc atcgccagta
     6661 ctgcaaggat aatgaccatg atcataaagc aggccaccag cgctatcacc ccggcggttg
     6721 gccccatctc ttctttgacc agctcaccca gcgatcgacc gtcacggcgc gtagaaacaa
     6781 acagcaccat gaaatcctgc accgcaccgg cgagaaccac cccggcgagt agccagatca
     6841 tccccggcag gtagcccatt tgcgctgcca gcaccggccc aaccaacgga cctgctccgg
     6901 cgatggccgc aaaatggtga ccgaacagca ctttcttgtc cgtcggcaca tagtccagcc
     6961 cgtcgttatg gcgcaccgct ggcgtcatac gcttcgggtc aaccgccagc acatttttgg
     7021 cgatatacaa cccataaaaa cggtaagcga tcagatagat acagaccgat gccaccacaa
     7081 tccacagcgc gttgatctgt tccccacgat ttaaagcaat gtatcccaga gcaaatgctc
     7141 ccattacaga gagcactgtc cagacgaggt atttccctga tttgttcata gttgttatcc
     7201 gtgagcgtgt ccatagagat gttacatttt gtttctataa catctttgta cgatttaaca
     7261 acacggtaac tatgtaaatc ggagttgaaa ccaatattta acttaacaat gttaactcga
     7321 tcactccgtt aaccgagttt tcctgcaatc tcatttaatt ctgtcgggct catcccaaaa
     7381 ctgccgtacc cagccgacaa gtgcagcaac gccgcccctg ttcatcgaaa acgacgattt
     7441 cccagctctg attttgccgc ccaagatgca gcggctggca gacgccgcgt accttgcctt
     7501 cagacaccgg gcgatgatgg gtggcgttaa gttccgtgcc caccacacac tgcccgtcac
     7561 gggtcatcat aaatccggcc atcgatccca gcgtttcagc cagcgccgcc gacgcgccgc
     7621 catgcagcag gccaaacggc tgatgagtac gggtatcaac cggcatttcg gcttccagca
     7681 catcatctcc cagacgggta tacacaattc ccagatgcgc caccattgtg ttatcgctgg
     7741 tggcgttcag ttcgtcgagc gttaaatggc gtttccagat catgcttatg cccccagcgt
     7801 tgagccgcca tcgaccacaa tatcctgtag ggtgatatgg ctggcgaggt cagaggcgag
     7861 gaacaaaatc gtgttggcga tctcttgtgg acgggcgatt ttccccagcg gaatgccgag
     7921 tttaaactgc tcgccaaagc cgcgaatacg ctgttcttcg gcgtcatcgc tcacccacag
     7981 cgtgcgttgc atatcggtgt cggtggagcc aggcgaaacc acattacagc gcacgccgct
     8041 acccgccagt tccagcccga cgctcaacgc caggcttttc agcgccgctt tcgatgcgcc
     8101 ataagcactc ataccaatac gcggcgtgtg tgcggcgtcg gacgccacag tgacaatcgc
     8161 cccgccccgc tgacggcgaa actggttcat ggtttgctgg aacaggttaa acgcaccgcc
     8221 gacgttaacc gcaaaagtct gctgccagtc ctctttactg agctgatcgg tcgcgcccat
     8281 gcgcaaaatt cccgccgcat tgatcagcac gtccagtcgc tccgtttcag ctaacagtcg
     8341 ctgacatacc tgcgccacct gcccggcgtc ggcaacatcc attacttcgg tcgcaaaggg
     8401 atattgctcc tgagtgaacg cttgatcaaa acctgtaact ttcgctcccg cctcaacaaa
     8461 cgccagcgcc gtggcgtagc cgataccttt acctgcgccg gttacccaga catttttacc
     8521 gctgaaatcc attatttaac ctcgcgggag agtagcttcc accaggcgtc gatggtcggg
     8581 tttttcgcca gcatgacaaa gtcgatatca ccatgcactt tgcgccagcg cgccgccagc
     8641 gccatcatgc gcaccgaatc cagaccgtag tcgatcaggt tgtcgtcatc gaacggttca
     8701 tcggactcgt ccagcaacgg caggataacc tcacgcagcg ccgctttgct ggcggggact
     8761 ggtgctggca gtaattcttc agtcatcacc acccggccag accgcccggc cacatatttc
     8821 agcgacatca aatgctcgtc acggctgaaa tcggccagcg catccgccac cataaacggt
     8881 ttaatatcgc gcataaatgc gtcggttgcg gtggtcatac agccaatgtg ggcatatacc
     8941 ccggtaataa tcagctggtt acgtccactc tctttgagca tttgctccag cggagaacga
     9001 tgaaacgcgc tgtagcgcca cttcaccagc accgtgtcgt cggcatctgg cgtcaggcga
     9061 tccaccacct tttgctgttc cggcgaacgg gtcaggcccg gcccccacat atcattcaac
     9121 agcgcccgat cttcatcgct ctgctctttc ggctgggcgg tgtaataaac cgggatattg
     9181 tgctgtttgc tgtagtcgcg cagcgcagca atattcgcga tcacctgctc catcatcggg
     9241 cagttctcgc cccagaagct gacaaaatag tcctgcatat catggattaa caacgcggca
     9301 cgttgcagtt caaaggccca gtcaacttta ttctgcggaa tatcgtgaga ctccggcagt
     9361 gcgtaagcct gtaattttgg aatagccatc gtgttctctc cttcaggctg atgcgcgtga
     9421 cgccagcctc tgacttaatt gttttttatc gactttcccg accgccgtca gcggaagtga
     9481 atctacacac tccacgcgat ccggtaattt aaattcggca ataccctgtt cacgcaggaa
     9541 acgacgcacc tgcaccgcgc gcagcggctc ttttaccacc agataagcac agcttttttc
     9601 gcccatcagc tcatcttcca tgctcaccag tgcggcatag atcaccgccg gatggcgcag
     9661 cagcaggttt tcgatctctt cggcagcgat cttctcgccg ccacggttga tctgatcttt
     9721 ctcgcgcccc tgcacggtga tgtaaccctc tggatcaata gagatcagat cgccggaaca
     9781 gtaaaaaccg ttggcatcaa aggcgctggc attgtgctgc ggacttttgt aataaccgcg
     9841 gaaggtgtac ggcccgcgcg tcatcaggcg tccgacttcc ccttgcggca gtggatttcc
     9901 ttcggcatcg gcaacccaca cttcatcgtc cggacacatt gggtaaccct gggtatggat
     9961 aattttctcc gcgctatcat caagacgggt gtagttcacc agcccttccg ccatgccaaa
    10021 cacctgctgc aactggcagc caatctcagc gggaatacgc gccgcaaggg tggcagaaag
    10081 acgtgcgccg ccgacctgta acagtttcag cgaggcaagc tgcgcccggc tttcgccttc
    10141 agccagcgcc tgcaaccaca ggctgactgc cggcggcacc agcgcggtga cgttcacctg
    10201 atgtttttca atcaatggga agcaaagcgt agcgctggga tcggccgcca gaacaaccgt
    10261 tcctccggca agaaagacgc ccagcgatcc tggcgaactc atggcgtagt tatgagccgc
    10321 cgggatcgcg cacaagtagc gcgtctgttg tgtgaactga caaatctcga cgctacgacg
    10381 cacgctgtag tagtagtcgt tatgagtgcg cgggatcagc ttcggtgtac cggtggtgcc
    10441 gccggaaagc tggaaatagg ccacttcatc agcaggtgat ggcgtggcag taaaaccgtc
    10501 tgccggatgg ttaatcgcat cctgcaagtt atgctcaccg ctgtcgttgt gcagttgcac
    10561 cacgcgaatg gaggaatgtt ctgtgacgaa agtattgagg aaatcatccc cgctaaacag
    10621 cgcatgttgg cgatcggcaa tcagcaatgc gggttcaatc tggctggcat aggcgttcag
    10681 ttcactacgc tgatggctga acaacgccag caccggcgca acgcccagtt tcagcagcgc
    10741 gaaaaaggta atatacaatt cagcgacgtt acccagttgt accagcgcgg tttcaccagg
    10801 tttaatgccc tgacggcgta aagtacacgc gaggttatcc gccgcctgat tcagctcccg
    10861 gtaactcaac tgtagctcgc cgtcgataac cgcgatgctg tcactcgcag cgtggcgagt
    10921 cagaatgtcg gtcagcggca aatcctgcca gtagcctttt tcccgatagc gacgggcaaa
    10981 ctcttccggc cagcgggtga atggaatgct catcctcgct ccttaatgca atccaaaaac
    11041 attcaacatg gtagaaagtt tgacgcctgt ttcgcgccac tcacccaacg gtgacgacgc
    11101 aggcacaatc cccgctccgg caaacagacg cacctgattt tcccgcagct tcgcacagcg
    11161 gatggtcacc acccattcgc cgttaccttc gctgtcacac caacccacaa tgccgccaaa
    11221 cagttcgcgg tcgaacggct ccagttcagc aataacctga gtcgcggcct gatgcgggaa
    11281 accgctcagc gcgggggtcg gatgcagcag acaggccaga gtcagtgcgt tttcttgcga
    11341 attcgcttta ccttcaaagg gagttgcgag atgccacagc gtcggcgtgg taatcaattg
    11401 tggagaggag ggaacgtgta actcactact gcgttcgcgc agtacctctt tcatcgcctg
    11461 agtcaccagt tcatgttcat ggcgatcttt ttctgacgcc agcagacgat tacccgcttc
    11521 gcgatcgagc acttcatccg gctgacgacg cgcggaaccg gctaacggaa tggagctaaa
    11581 acgctcgccg tctttacgta gcagcagttc cgggctggcc cccagcagga cgccaccatc
    11641 agccagcggg atatggaagt tgtaactaac cgggttttgc gcaatcaacc gttccagcaa
    11701 tacgccacta tcaatggcgg cgtcagtggt gatatcaatc aaccgtgaca acaccacttt
    11761 gtcgacctgc ggcgtggcgg taagtgcggc agcgcgggca accatctgtt caaacgtggt
    11821 ttgttccgga attgcctggc gttccaccac attcagcgac tggctgcggg tgaaacggcg
    11881 ggcggaagct tgtttttcct gacgggagaa cgactgccag gattcaggaa tatacagcga
    11941 cgaaggctga cgtggatcga agggaatcgc cccgaccatc accggatttt tgatgccctg
    12001 cactttggca tcggcaaaca gcgcggcgag tttttgctgg aagggactgt cgggcgaatc
    12061 cccgttcaca gccggttcat cgaagcgggc gaaacatcct gacgtcgtaa aactgcggta
    12121 cggcgacata aagaaaaagc gattgggcgc aagtgttgcc atggtctgct gtacttcctc
    12181 agccagtgac gtatccatat catcctccac aaaataataa aggctttaat aatgattatc
    12241 atttatattt tcggtcgcta acctaaaagc aaacgcgcac tatgtcaact cttgaggtaa
    12301 cgcaatctgc aactcggggt tgcatctggt tgtggttaca atgaaaatga gaagcattat
    12361 tgatggattc gcataagcgc aatgtgatgt cctgcgccgt tcttccccct ctcccttcca
    12421 gggagagggc tggggtgagg gtcaatattc gcaccagtgc tggctgttcc catcacccta
    12481 accctctccc caaaggggcg agggtacgga tagtgcgctt tgtcgaattt gtcattacgc
    12541 ccttaacctt attaataaca ggaagctgat ttgtgagact cgccccgctc taccgcaacg
    12601 cccttctatt aacaggactt ttgctttccg gaatagccgc agttcaggcc gccgactggc
    12661 cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg cagcgtattg
    12721 tttccaccag cgtcaccctg accggctcac tgctggcgat tgatgctccg gtgattgcca
    12781 gcggcgcgac cacgccaaat aaccgcgtcg cggatgacca gggcttttta cgccagtgga
    12841 gcaaggtggc gaaagaacgc aaactacaac ggctctatat cggcgaaccg agcgccgaag
    12901 ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg gattcggcgc
    12961 tggcgctgta tgatcagctt tctaccatcg ccccgacatt aatcatcaat tacgacgaca
    13021 aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg
    13081 cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa atcaaattac
    13141 cgccgcagcc ggtcactgct attgtctata ccgccgctgc acacagtgcc aatctctgga
    13201 cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg gcgaaattgc
    13261 ccacagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt cagcttggtg
    13321 gagaaaatct ggctgcaggg ttaaatggcg agtcactatt cctgttcgcc ggtgatcaga
    13381 aagacgccga tgcgatttat gccaatccac tgctcgcgca cctgcccgca gtacaaaaca
    13441 agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc gccatgcaag
    13501 tgctggaacg acttaaggcg ctgttttaag cattaactgt cggacgctgt cacctgcggc
    13561 ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac gccgataatc
    13621 aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc tcccagaccg
    13681 cctaacagcg ccgcgcctat ggcatcgccc gtcacgtttt gtgccgtcca caaaccgttg
    13741 attcgcccta acattgcttc cggcgtttgc gtttgcagca ttgtatattg cagcaacgaa
    13801 ctgaccgcac tcagccagcc gaacagcacc agacaaacca cgcctaaaat ccacatcggc
    13861 atcaggccaa ataaaccaat ggcgaggaac gatcccaacg tggagagcaa catcaacaat
    13921 cctggtcgcg cgctatgcgc cagtttccca ctggttaatg cgccaatagc cgcaccgagc
    13981 gggatcgccg catagagaaa accaatctct gccgccgaca tctgccagtt gtcagccagc
    14041 gccggataca gtacccgcac cgcgctcgcc atcgtcaata aaccacccag caacgcaatc
    14101 ccgcccacca gcgggctggc gagcagaaaa cgaaatcctg ccagtaatga tttcaacgga
    14161 tgctcgcgcg gctgcggtgg cggtggcaac gccggaaggc ttaacaacgg tagcaaggta
    14221 ataaacgtgc ccgccgccgc cagcccgtag ttccaggcta cgccaccggt cgccagcaat
    14281 aaaccgccaa tcatgggcga aatcaccgac cccagacgca cggtcaacat ggtgatcgcc
    14341 ccggcctgca ttaagttttc acgccctacc agtgctggtg tcgccgccaa tagcgctgta
    14401 acgccaagcg atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac
    14461 ggctccggca gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc
    14521 gccagcaaaa tcactttttt gcgctcatag cgatccgcca gcacaccgcc aaccatcagg
    14581 ccaacaaaca tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgc
    14641 gtcatcatct ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag
    14701 ataaaacgag cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta
    14761 agcagccagg attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt
    14821 cttaggctgc acatgctaac atatccaaat aagatcgata acgataatta atttcattat
    14881 catggaagtt cgtatgtctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt
    14941 gctgttatta ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc
    15001 cgcttccgta gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt
    15061 gctcgacgcg cgactgccgc gcacccttgc cggtttactg gcaggcggcg cgcttggcct
    15121 tgccggggcg ttgatgcaaa ccctcacccg aaatccactt gccgaccccg gcttgcttgg
    15181 cgtgaacgcc ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc
    15241 cgcgcaggaa caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc
    15301 ctttaccggc agtcagggct gcgggcagtt aagtccggtg cgtttaaccc tggcgggcgt
    15361 ggcgctggcg gcagtgctgg aagggctgac cagcggcatc gccctgctta atcctgacgt
    15421 ctacgatcaa ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt
    15481 aaaagtggtg ctgatcccgg tgctgatcgc cggagcaacc gcgctattac tgagccgcgc
    15541 gctgaacagt ttaagcctcg gcagcgacac cgcgacggcg ctgggcagtc gcgtggcgcg
    15601 cacacagtta attggtctgc tggcgattac cgtgctttgt ggtagtgcga cagcggtagt
    15661 tggcccgatt gcctttattg gcctgatgat gccgcatatg gcgcgttggc tggtgggtgc
    15721 cgatcatcgc tggtcgctgc ccgtaacgct gcttgctacc cctgccctgc tgctgtttgc
    15781 cgatatcatc gggcgggtga ttgtgcccgg tgaactgcgc gtttctgtgg tcagtgcctt
    15841 tattggtgca ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt
    15901 tacgtctctc gccgattact catcacctgt ttgctgctta tttccgcctg tgtggttgca
    15961 ggtatctggg gattacgcag cggtgccgtc acgctggaaa cctcgcaggt attcgccgcg
    16021 ctgatgggcg atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc
    16081 gtgctgatgg cgctgttgat tggcgcggca ctgggcgtca gcggtgcgat ttttcagtcg
    16141 ctgatgcgta acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc
    16201 ggcgtgctgg tggcgatggt gctgtttggt caggacctga cggctatcgc gctggcagca
    16261 atggtgggcg gcattgtcac ttctctgttg gtctggctgc tcgcctggcg taacggtatc
    16321 gacacctttc ggttgattat tatcggcatc ggcgttcgcg ccatgctggt ggcctttaat
    16381 acttggctgt tgctgaaagc gtctttagaa acggcgctaa cagcgggttt gtggaatgcc
    16441 ggatcgctca atggcctgac gtgggcaaaa acttcgcctt ccgcacccat cattatattg
    16501 atgctcattg ccgccgcctt gctggtgcgg cgaatgcgct tgctggagat gggcgatgat
    16561 accgcctgcg cgctgggcgt cagcgtcgag cgttcgcgtc tgttaatgat gctggttgca
    16621 gtggtgctta ccgctgcggc aacggcgctt gccgggccga tttcctttat tgctttagtc
    16681 gcgccgcaca ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg
    16741 ctgtgcggag cgctgttact gctggcggcc gatctctgcg cccaacaact gtttatgccg
    16801 tatcaacttc cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg
    16861 ttaattcagg agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt
    16921 taaccctggg atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg
    16981 gtcacttcac ggcaattatc ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct
    17041 taagccgcct gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac
    17101 attacgccag taaagaagtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc
    17161 cgggcgatat caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt
    17221 ttacccactg gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa
    17281 taactcatct ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt
    17341 ggatcgcgat ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct
    17401 ggctggatat cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga
    17461 aaggctatac cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc
    17521 atttgattgc attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca
    17581 ctgctgaact gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag
    17641 ccggaacgcc gcttgtggtg ccgctcgaac gaacggcacc ttcaaccgca aatagttaaa
    17701 ctaagtggtc tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca
    17761 cgctaccaca agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac
    17821 ccggaccgtc ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat
    17881 tcacatcgtt gacatttgct tttgttaatt gctcgacaag atactgccga ttacgtaatt
    17941 caccgttcag ttccgcaacg tcagtaaccg ctttttcaat ttccagtttg cgttcaatac
    18001 cgtatgcgcc gagagaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt
    18061 atacgggctt tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa
    18121 tgtttgcatc aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa
    18181 actgggtttt gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg
    18241 cagagatata atcgatatac ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg
    18301 taaaacttag cgtccaggag gtatacagtg acggttcatc ttttttctta ctggcattgt
    18361 catcaaccgc tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat
    18421 cgattttcgc ctcttttaat tggtccatca cataaggtga tgaacgcaag tactcttcca
    18481 gcaagctaac agactgaaac ttcttgataa acaggttaaa tgcaccgcca cggtcgatat
    18541 taacatcgag atcaagaacg cggagtttgg taaaggtttt ctccaggtcc tgccactgaa
    18601 tggcttcggc gggagtaata acggctgagc tggtccattt ttgcggcagg atgaaagaga
    18661 tcagtaaacc tgcgcaggca aatgcaaaaa cgaccgccat gaccgttttt ttggcccgcc
    18721 ataaaacttc gattagatta agtaaatcaa tttcattact actgggcgac gccagaggat
    18781 actcggaaaa atgagcgtca cttccctgtt taatattcag tgatgacatg ggtataacct
    18841 gaaaatgagt ccaatagggg gaaatctcca cggcgctcac tttaccagtt ggtgagaatt
    18901 atccgaagct gaagtttgta aattccttcc actgaatgca acattcactc tatttaagag
    18961 aattattcat caaacctata aatcaaacaa ataaaaaacc cataattatt ttctgtaatt
    19021 atgggtttat agataatatt aatttacctg tttagcgttg cgcgaataat cggcccaatt
    19081 tttacaaacg cccctggaga gataatatcc acatgcgcac aatcctgacg atagatatcc
    19141 agctcggcaa tccagggcga ccaagcgcgt tcggggctca taccttcctg aagcgtgcgt
    19201 tcagcaacaa acagcgtcgc tttaccatca aacggtacgc tatgagcagt cgtcagcaga
    19261 cgcacagcat cagcgtagtt gccttcaatg gtggtaaaca actccgttga agtacttccc
    19321 tgctgtgccg ccaggaaggc ctcgcgttcg cggttaatct ccgccagcac ttccgggtcc
    19381 agaccattag cctctttttc ctgccagttt tgcgtttctg gcggccaggt atccagcaaa
    19441 ccaagaaatg ccacctgttc gccacgggca cgcagtcgcg ccgcaatgcc ctgcgccagc
    19501 gtaccgccaa gggaataccc cagcaggtaa taagggccgt gcggttgttg ttcaagtaac
    19561 gttgccagat gcgcttcaca gacttcatcc agatttgccg cggtatgcat ggggccattg
    19621 gggcgcggcg actgaatgcc gataatcgac cattgtggat cgatataacg cgagagcacg
    19681 ctaaactgcc aggcaaaacc ggatgccgga tggaaacaaa acagcgtcgg gccattacct
    19741 tcacgcaacg gcagaatggt ttcgaatccc atacgccggg tgctgtcttc ttcagcatca
    19801 ataatcgttg ccagtttagc gacggttgac gcgaccatca cttgccccgg cgtcacctgg
    19861 cgggcaacct gccgacttaa ctgcgctgcc agtttcattg ccagtagcga atgaccgcca
    19921 agcgcaaaga aatcagcatc ggcatcctgc acctcacaac ccagcaacga cgcgaatgcc
    19981 gcggcaataa tcgtttcact gcccgctttt ggcgcacgcc ccggcgcttg tgccttcagt
    20041 tcaggcaacg gtaaggcttt gcgatccagc ttgccgttgg cgctaagagg taacagtgga
    20101 agttgcagca gaaccacggg caccatatgt ggtggcaatg tttcgcgaag ctgcgcctgt
    20161 aatgcgctgg tatccaacgg cagacctgat tgcgacacca gatagcccac caactgacgc
    20221 gcatcaccac cggtggctgc cgcctggtta atcacacagg cgtgggtaac ggcttgttcg
    20281 acatccggca gcgcctgcat cacgcgatcg atttcgccca gttcaatacg ctgcccgcga
    20341 atttttagct gatcgtcact gcgcccgagg tactccaccg cgccgttatc cagccagcgg
    20401 gcaacgtctc cggtacggta catccgttca ccagggacaa aaggatcggc aataaagcgg
    20461 ctggcggtca gatcgggtcg tccaagatac ccctgcgcca gttgaatgcc ggtgagatag
    20521 agatcacccg ccacacccgg cggcaccgga tgcatcatcg catcaagaat acgcagaccc
    20581 gtattccata ccggataacc aatcggcaca ctgctgccgc gcacctgtgc cagttcctcg
    20641 ccaaaagccg gataccagct gacatctacc gccgcttccg tcgggccata tagattatgc
    20701 aacggcacgc tggttaactg ttgccattcg cggcataaat cagccggtaa ggcctcacca
    20761 ctacagaaaa cctgtttcaa cgtcgcgcaa ctctggcgag cggtttgcgg cgtcagcgat
    20821 gcaacaaatg ctgccagcat cgacggcaca aagtgcgtgg tcgttacgcc atattcggca
    20881 aagaattgct gcatagcgag cgggtcgcgg tgcgcttccg gttcagccat caccagttta
    20941 gccccggcaa taaacggcca gaaaaactcc cacaccgaga catcaaaact gcacggcgtt
    21001 ttttgggcaa cgacatcttc gcctgtaagc agataatgat tttgcatcca caacaggcgg
    21061 ttaacgatag ccgtctgccc gaccattacc cctttcggcc tgccggtgga gccagaggta
    21121 aagatgatat aagccgtgtg atgcggttgt gaaagttgca gcggcgcact gccctgcggt
    21181 ttaagcgggg cgttatagca aaggttggtt aaatctggaa catcggcaaa gcgcggcagt
    21241 tgatcgtcgg tggtgattaa cagcgacgga cgcgcatctt ccagcatcat tttcaggcga
    21301 tcgtccggat agccggtatc cagcggtagc caggccgcgc cagcttcaac tatcgcatgt
    21361 agcgccaggg tcaaaaagac cgagcgcggt agcgccaccg ccacgctgtc gcccggttta
    21421 acgccacgct cacgcagcag attcgccagc gccaccacct gctcgcgcat ttcccgatag
    21481 ctgaactggt aacgcgcatc tgccagcgcc ggagcatccg gtgtttttgc cgcttgttct
    21541 gccaccagcg cgctcagcgt ggtttctgga atctcaacct gagtggcgtt gatctgcgcc
    21601 agctgcgcat actcacctgg cagcataata tcgacatcgc cgcacaacag cgccggatcc
    21661 gcggcgaact gggcaatcag cattttcagg cgttcagcat gctggattaa cgttggctca
    21721 tcgtaacgct gtttattggc gaggatctca atactcaaat caccgtgttc atccgggaac
    21781 agggccagtt caaggtcatt aaccggaccg gttgccaagg tatgggtttg cgcctgaaca
    21841 ccaggaatat ccagttggta atcaaatacc ttgatattga gtaccggacc aaacagcggt
    21901 tcatcacctg ccgctcgccc gctgtcacgg acaatttgtt cggcatcgta acgttgatga
    21961 cgacgcattt ttttcagttg tgctgccagt cgggttgcca gttccggcag cgtttcttgc
    22021 gccgcaatgt gaatacccaa cggcaaaacg ttgagcacgg gtccggtagc cgtcagcgcc
    22081 gccgagccca gtcgacgcat aaagataaat ccagcggcgt agtccatgcg attgcacaat
    22141 cgccccagcc acaaagccgt cagcgcaagg gctaaatcgg tacgctgcac acctgaaagt
    22201 tgcgtagcca actggcggaa ttccccgtcg gtaaattcca ttttcagacg taaaatatct
    22261 gccgaagcgc tgcgccccgg taaaggtgcc ggagaaagtg acgcgggcgg cggcagttga
    22321 cgacgctgtt ctgcccagaa tgccgcatca cgctgccagg cttcgctttc gcggtattgc
    22381 tggtactctt ccactacatc agcgaaaggc gtaaacggcg aagcaggcgt tggttcgcca
    22441 cgcagccatg tgcagtaaat attggcgatc tggcgggtaa tggccgggaa actgaagcca
    22501 tcgaccagca aatggtgata acgctgatac cagtaccagc ggttatccgc cacctgtatc
    22561 agctgatgaa agaccagtgg tttaccgcta tcgacgcgca gatcttgttg caaatccgcc
    22621 tgcattaatg cctgcgcagt accgtgcgga tcaatgttgg ttcgcaggtc gataatttct
    22681 ggcagttcga acgtcagcgc atcatcgacc cactgccaga cttcgccgtt atcttccgta
    22741 aaacgcatcc gcagcgtatc tgcttgcgct agtccgtcaa ccaccgcgcg ggccagtaat
    22801 ggcgcatcaa cctctccggt taactcaacg taatgcgcta cgctccaggc ggagggtaaa
    22861 tccgacagtt tttctgccat ccagatgccg ggctgtgcgg cgaccaaagg taaatgctgg
    22921 ctcattgcgc ctcctgcaac tgggtgaaat tcgccggtgt cagagtacgc cagtgggctt
    22981 caagccactg ctggcaggac tcttgtgact gcggctgaca cacaatgtcc cagcctgccg
    23041 gtaaggcgca ttgttgcggc cacagactga attgcccctg cgcattgcgc aaaatgtaaa
    23101 acgctccctg tggatcatcg aagggattac tgaatgccat attcaactcc tgtcatggaa
    23161 aagtggttgc cagaggtcga ttagcccctg catcaagcca ccgcgccaac aaagcgcatc
    23221 atgtccgccg tcaacctgac gccagaaaat ggattctttt atggggtgta attgcgcata
    23281 cagcgcctga ttggcccgca taatcatcgg ctcgcgaata cccgcttcca gcacaatgcg
    23341 cagaccttcg gcgctaactt caccagcttt aagcttttca agtaacacgc cctcttgctg
    23401 cccgccccga tgcggccacc agtacgatcc tgactggctt aatacacaac caaaacgttc
    23461 aggccagtgc agtccggcat acagcgcgga aagtccacca aaactctgcc cggcaaccac
    23521 ggtgcgatcg gcgcgatcgc taaaaggggc aatagctttc accaggggta ataactcttg
    23581 ctgtactgcg agccagaaat ccgcattaca cggcagttcg tgagcgcggt gcgtggtgtc
    23641 gatagcgtcg atcaacacat acacggcggg aggaagttgc tgacgatggg tcagcgaagt
    23701 cagcactggc cagacgggca tactttgcgc ccaaaattcg ccatcgagca aaactgccag
    23761 cgggcgttct tctgctgttg catcgccggt ggtaaaaatc catacacgac gtgaattttt
    23821 caaccgttca cttttccaga taatttcttt ggctggtatt tctggcgctt gcggacaatc
    23881 ccatcccggt tgcagaggcg cttgcggcat ttcgagtgca gaaacagcgt gccctcgccc
    23941 gcctttccag ctttgtggat tcagcggatc ggctattgcc tggggtaata gttttcgcca
    24001 gccttcacgt aattcgaggc gatcggggga tggtgcagaa aaaatgtcat cgcgttcggt
    24061 gggaataaag cagtagctgc cgcgccagtt ggcattgagt tgtgtcgtcc actgccagac
    24121 atcagtacct gcaatgcgct gcatcgactg gggctggctg ttctgatgat gatcggtcac
    24181 accagtgatg tagacccata cgcgctttat cggcgagtat tcttcagaac cttggggatc
    24241 acgccaccag aaagtgacct caaacatttc gtcattcaga cgctgccatt ccgggccatg
    24301 tttcgactgc caccagctct cacttcctac ttttaacgcc gtcacaccat aaccccatgt
    24361 ttactgtgca atttttcatt gattgcagaa atatattgat aatattattg ataactattt
    24421 gcatttgcaa tagcgtaatg gcgcgccgtg ggaagcgcgg acattaatta accaactgca
    24481 ctgcgtgtct ttcaggatca aaggttttcg cggtagcggg atgcgtcgtg ttgaggacga
    24541 ccatgcccga cagttgcaat tcgtggcaaa aatgcaggaa taaaacaatg aacaagaaga
    24601 ttcattccct ggccttgttg gtcaatctgg ggatttatgg ggtagcgcag gcacaagagc
    24661 cgaccgatac tcctgtttca catgacgata ccattgtcgt taccgccgcc gagcagaact
    24721 tgcaggcacc tggcgtttcg accatcaccg cagatgaaat ccgcaaaaac ccggttgccc
    24781 gcgatgtgtc ggagatcatc cgtaccatgc caggcgttaa cctgaccggt aactccacca
    24841 gtggtcagcg tgggaataac cgacagattg atattcgcgg tatgggtccg gaaaacacgc
    24901 tgattttaat tgacggcaaa ccggtcagca gccgtaactc ggtgcgtcag ggctggcgtg
    24961 gcgagcgcga tacccgtggt gatacttccc gggtgccgcc tgaaatgatt gaacgtattg
    25021 aagttctgcg tggtccggct gcagcgcgtt atggcaacgg cgcggcgggt ggcgtagtta
    25081 acatcattac caaaaaaggc agcggtgagt ggcacggttc ctgggacgct tatttcaatg
    25141 cgccagaaca taaagaggaa ggtgccacta aacgcaccaa cttcagtctg accggcccgc
    25201 tgggcgacga attcagcttc cgcttgtatg gcaacctcga caaaacccag gctgacgcgt
    25261 gggatatcaa ccagggccat cagtccgcgc gtgccggaac gtatgccacg acgttaccag
    25321 ccgggcgcga aggggtgatc aacaaagata ttaatggcgt ggtgcgctgg gacttcgcgc
    25381 ctctgcaatc actcgaactg gaagcgggtt acagccgcca gggtaacctg tatgcgggcg
    25441 atacccagaa caccaactct gacgcttaca ctcgctcgaa atatggcgat gaaaccaacc
    25501 gcctgtatcg ccagaactac tcgctgacct ggaacggtgg ctgggataac ggagtgacca
    25561 ccagcaactg ggtgcagtat gaacacaccc gtaactcgcg tattccggaa ggtctggcgg
    25621 gcggtaccga agggaaattt aacgaaaaag cgacacagga tttcgtcgat atcgatcttg
    25681 atgacgtgat gctgcacagc gaagttaacc tgccgattga tttcctcgtt aaccaaacgc
    25741 tgacgctggg tacggagtgg aatcagcaac ggatgaagga cttgagttcc aatacccagg
    25801 cgctgaccgg ggcgaacacc ggcggcgcta ttgatggtgt gagtgccacc gaccgtagcc
    25861 cgtattcaaa agcagaaatt ttctcgctat ttgccgaaaa caacatggag ctgactgaca
    25921 gcaccatcgt aacgccgggg ctgcgtttcg atcatcacag tattgtcggc aataactgga
    25981 gcccggcgct gaacatatcg caaggtttag gcgatgactt cacgctgaaa atgggcattg
    26041 cccgcgccta taaagcgccg agcctgtacc agactaaccc gaactacatt ctctacagta
    26101 aaggtcaggg ctgctatgcc agcgcgggcg gctgctatct gcaaggtaac gatgacctga
    26161 aagcagaaac cagcatcaac aaagagattg gtctggagtt caaacgcgac ggctggctgg
    26221 cgggcgtcac ctggttccgt aacgattatc gcaataagat tgaagcgggc tatgtggctg
    26281 tagggcaaaa cgcagtcggc accgatctct atcagtggga taacgtgccg aaagcagtgg
    26341 ttgaaggtct ggaaggatcg ttaaacgtac cagttagcga aacggtgatg tggaccaata
    26401 acatcactta tatgctgaag agtgaaaaca aaaccacggg cgaccgtttg tcgatcatcc
    26461 cggagtacac gttgaactca acgctgagct ggcaggcgcg ggaagatttg tcgatgcaaa
    26521 cgaccttcac ctggtacggt aagcagcagc cgaaaaaata caactacaaa ggtcagccag
    26581 cggttggccc ggaaaccaaa gaaatcagtc cttacagcat tgtgggtctg agcgcgacct
    26641 gggatgtgac gaagaatgtc agtctgaccg gcggcgtgga caacctgttc gacaaacgtt
    26701 tgtggcgtgc gggtaatgcc cagaccacgg gcgatctggc aggggccaac tatatcgcgg
    26761 gtgccggggc gtatacctat aacgagccgg gacgtacgtg gtacatgagc gtaaacactc
    26821 acttctgatg ctaacgtcag attgttgaca aagtgcgcgt cgttcatgcc ggatgcggcg
    26881 tgaacgcctt atccggccta caaaaatctt gccaattcaa tatattgcag gaccatgtag
    26941 gcctgataag cgtagcgcat caggcagttt agcgtttgtc atcagtctct aatatggtcg
    27001 acatgaaaac tacgcatacc tccctcccct ttgccggaca tacgctgcat tttgttgagt
    27061 tcgatccggc gaatttttgt gagcaggatt tactctggct gccgcattac gcgcaactgc
    27121 aacacgctgg acggaagcgc aaagccgaac atttagccgg gcggatcgct gcaatttatg
    27181 cgctgcggga atatggctat aaatgtgtgc ccgcaatcgg cgagctacgc caaccggtgt
    27241 ggcctgcgga ggtatacggc agtattagcc actgcggggc tacggcattg gccgtggttt
    27301 cccgtcaacc gattggcgtt gatatcgaag aaattttttc tgcgcaaaca gcgaccgaat
    27361 tgacagacaa cattattaca ccagcagaac acgagcgact cgcagactgc ggtttagcct
    27421 tttctctggc gctgacactg gcattttccg ccaaagagag cgcatttaag gcaagtgaga
    27481 tccaaactga tgcaggtttt ctggactatc agataattag ctggaataaa cagcaggtca
    27541 tcattcatcg tgagaatgag atgtttgctg tgcactggca gataaaagaa aaaatagtca
    27601 taactctgtg ccaacacgat taattgacaa catctggtac gattcgcccg cagccatcac
    27661 tgaccgcggg cgaaagtgta aagcaggtgc cttaccactc t
//
LOCUS       DAJTNA010000036        27505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTNA010000036 DAJTNA010000000
VERSION     DAJTNA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27505
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.036"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..588
                     /locus_tag="OO161_001301"
     CDS             <1..588
                     /locus_tag="OO161_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5462438.1"
                     /translation="RNHYEYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMY
                     CTGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGE
                     RISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPP
                     EEQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
     gene            complement(630..1205)
                     /locus_tag="OO161_001302"
     CDS             complement(630..1205)
                     /locus_tag="OO161_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5462439.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1209..1775)
                     /locus_tag="OO161_001303"
     CDS             complement(1209..1775)
                     /locus_tag="OO161_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5462440.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            complement(2036..2149)
                     /locus_tag="OO161_001304"
     CDS             complement(2036..2149)
                     /locus_tag="OO161_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5462441.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            complement(2197..3315)
                     /gene="solA"
                     /locus_tag="OO161_001305"
     CDS             complement(2197..3315)
                     /gene="solA"
                     /locus_tag="OO161_001305"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5462442.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRSGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            complement(3430..3684)
                     /gene="bssS"
                     /locus_tag="OO161_001306"
     CDS             complement(3430..3684)
                     /gene="bssS"
                     /locus_tag="OO161_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5462443.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            complement(3973..4218)
                     /gene="dinI"
                     /locus_tag="OO161_001307"
     CDS             complement(3973..4218)
                     /gene="dinI"
                     /locus_tag="OO161_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5462444.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            complement(4292..5338)
                     /gene="pyrC"
                     /locus_tag="OO161_001308"
     CDS             complement(4292..5338)
                     /gene="pyrC"
                     /locus_tag="OO161_001308"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5462445.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            complement(5444..6004)
                     /locus_tag="OO161_001309"
     CDS             complement(5444..6004)
                     /locus_tag="OO161_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5462446.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            complement(6138..6785)
                     /gene="grxB"
                     /locus_tag="OO161_001310"
     CDS             complement(6138..6785)
                     /gene="grxB"
                     /locus_tag="OO161_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5462447.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            complement(6849..8057)
                     /gene="mdtH"
                     /locus_tag="OO161_001311"
     CDS             complement(6849..8057)
                     /gene="mdtH"
                     /locus_tag="OO161_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5462448.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIESIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            8292..8876
                     /gene="rimJ"
                     /locus_tag="OO161_001312"
     CDS             8292..8876
                     /gene="rimJ"
                     /locus_tag="OO161_001312"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5462449.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFTNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALAAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTLDWTPGR"
     gene            8887..9534
                     /locus_tag="OO161_001313"
     CDS             8887..9534
                     /locus_tag="OO161_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5462450.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTQEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            9536..10402
                     /locus_tag="OO161_001314"
     CDS             9536..10402
                     /locus_tag="OO161_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5462451.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            10569..12104
                     /gene="murJ"
                     /locus_tag="OO161_001315"
     CDS             10569..12104
                     /gene="murJ"
                     /locus_tag="OO161_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5462452.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLM
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSCVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(12144..12560)
                     /gene="flgN"
                     /locus_tag="OO161_001316"
     CDS             complement(12144..12560)
                     /gene="flgN"
                     /locus_tag="OO161_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5462453.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(12565..12864)
                     /gene="flgM"
                     /locus_tag="OO161_001317"
     CDS             complement(12565..12864)
                     /gene="flgM"
                     /locus_tag="OO161_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5462454.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSVT
                     LSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADALINEAQQDLQSN"
     gene            complement(12940..13599)
                     /gene="flgA"
                     /locus_tag="OO161_001318"
     CDS             complement(12940..13599)
                     /gene="flgA"
                     /locus_tag="OO161_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5462455.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGLVVSGVVDADGNILIN
                     L"
     gene            13755..14171
                     /gene="flgB"
                     /locus_tag="OO161_001319"
     CDS             13755..14171
                     /gene="flgB"
                     /locus_tag="OO161_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5462456.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            14175..14579
                     /gene="flgC"
                     /locus_tag="OO161_001320"
     CDS             14175..14579
                     /gene="flgC"
                     /locus_tag="OO161_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR5462457.1"
                     /translation="MALLNIFDIAGSALTAQFQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            14591..15286
                     /gene="flgD"
                     /locus_tag="OO161_001321"
                     /pseudo
     CDS             14591..15286
                     /gene="flgD"
                     /locus_tag="OO161_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            15311..16519
                     /gene="flgE"
                     /locus_tag="OO161_001322"
     CDS             15311..16519
                     /gene="flgE"
                     /locus_tag="OO161_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5462458.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVNGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFATLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILKTLVNLR"
     gene            16539..17294
                     /gene="flgF"
                     /locus_tag="OO161_001323"
     CDS             16539..17294
                     /gene="flgF"
                     /locus_tag="OO161_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR5462459.1"
                     /translation="MDHAIYTAMGAASQTRNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGC
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            17432..18214
                     /gene="flgG"
                     /locus_tag="OO161_001324"
     CDS             17432..18214
                     /gene="flgG"
                     /locus_tag="OO161_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5462460.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            18267..18965
                     /gene="flgH"
                     /locus_tag="OO161_001325"
     CDS             18267..18965
                     /gene="flgH"
                     /locus_tag="OO161_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5462461.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNVRADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            18977..20074
                     /gene="flgI"
                     /locus_tag="OO161_001326"
     CDS             18977..20074
                     /gene="flgI"
                     /locus_tag="OO161_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5462462.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            20074..21015
                     /gene="flgJ"
                     /locus_tag="OO161_001327"
                     /pseudo
     CDS             20074..21015
                     /gene="flgJ"
                     /locus_tag="OO161_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            21081..22724
                     /gene="flgK"
                     /locus_tag="OO161_001328"
     CDS             21081..22724
                     /gene="flgK"
                     /locus_tag="OO161_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR5462463.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            22736..23689
                     /gene="flgL"
                     /locus_tag="OO161_001329"
     CDS             22736..23689
                     /gene="flgL"
                     /locus_tag="OO161_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR5462464.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(23885..27070)
                     /gene="rne"
                     /locus_tag="OO161_001330"
     CDS             complement(23885..27070)
                     /gene="rne"
                     /locus_tag="OO161_001330"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5462465.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRYERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
BASE COUNT         6668 a         6939 c         7319 g         6579 t
ORIGIN      
        1 cgtaaccact atgagtatga agtggggcac tttgaaaacg cgctcgaaat tccggcagat
       61 accttccgtg agcagctgcc aaaagcagtc gagatgatgc aggcacataa agataaaaaa
      121 atcgtcatgt actgcaccgg cggcattcgt tgtgaaaagg ccagtgcttg gatgaaacat
      181 aacggattca acaaagtctg gcatatcgag ggcggaatta ttgaatacgc ccgtaaggcg
      241 cgcgagcagg gcttgccggt gcgttttatt ggcaaaaatt ttgtttttga cgagcggatg
      301 ggcgaacgta tatcggatga gattatcgcg cattgccacc agtgcggcgc gccgtgcgac
      361 agccatacca actgtaaaaa tgatggctgc cacctgctgt ttattcagtg tccagtatgc
      421 gcggaaaaat acaaaggttg ttgtagtgag atttgctgcg aagaaagcgc gttaccgcca
      481 gaagaacagc gacgccgtcg ggcaggacgt gaaaatggca ataagatctt taataagtct
      541 cgtggacgtc tgaatacaac actgggcatt cctgatccaa cagaataaat atcattgccg
      601 gatgcgtgcc atccggcaac atttcacgct tacttctgct gtacgccttc cactgaaata
      661 atcagatcca cttcctgaga agctggacct aaatctgtct tgatattgaa gtccttgagc
      721 ttaattttgc cttcggcctc gaagcctgca cgtttaccac cccatgggtc gtcgccctga
      781 ccaattaatt tcgcttccag cgtgacgggt ttggttacgc cattcagcgt cagatcgccg
      841 gtaatatcca gttcgtcacc gtctttcttc acgctggtgg aggtgaatgt tgcctgtgga
      901 tattttgcgg tattgaggaa atctgcactg cgaagatgtt tatcgcgttc ggcgtgatta
      961 gtatcgacgc tggtggtgtt aattgtcaca ttcactttat cggcagccgg atttttttcg
     1021 tcaaaggtaa aagtaccgtc gaaatcttta aaggtgccgt ataaccagct atagccaagg
     1081 tgctggatgc ggaaattaac aaaggcgtgc tgaccttctt tgtcaatttt gtaatcggcg
     1141 gcaaccgctg aaccggcaga gaacatcagg gacgcgaagg ttaaaccaag caggcttttt
     1201 ttcatttttt atactccata gtcagatgac gactttccca gcatgcgctt cagagtgtcg
     1261 tctttatcga tgaaatgatg cttcagggcc ataaatccgt gcataacgga cagtactacg
     1321 acgctccacg cgagccaaaa atgcagggca ccggcaaagt ctgcctgtgc gccagcgtcg
     1381 gcaagggtcg cggggacgtc aaaccagcca aaaacgctga tcggtttacc atcggcagtt
     1441 gagatcagat agccgctgat gccgatggcg aacagcaata ggtacaatgc gagatgtcca
     1501 gcctttgcgg caagacgagt catgggcgaa taactcggca gcggacccgg cggtggagat
     1561 atgacacgcc acagaacacg aataaccagc cccatcatta acaaaatacc gatgctttta
     1621 tgcagttcgg gtgctttgtg ataccagcca tcgtaataac tgagcgtgac catccacaga
     1681 cctaaagcaa acatgccgta gacaataatt gcgcttagcc aatggaaggc cgcagatata
     1741 acaccatagc gttcaggggt atttgtgaat gacataagtg catccaatac gaatttgcga
     1801 gagaaagaaa atggcgtgtc ggagaatgaa ttgcaacaca aaaataacaa attgaatgat
     1861 atttattttt atttcaataa ttttgaatta ataatgaaag aatcttcata agtttagtgg
     1921 gttcagcatc agagatgtct ggtgagtgct gtagaagcag gaataatctc attcaatttt
     1981 tcacgctaaa tgaaagggag attaaatatt tgtcagttta tgtcagtgcg gaaaactaaa
     2041 aataaaacac atagatcaga tccataattg ccaacaatcc ccagagaaaa agataaagca
     2101 tcagatgctc gcgaatatta ttgatgagat agtgcaacag ccgacgcatt aaatactcct
     2161 gtaaaaacgg cctcattctg gaggccgtaa tgatgattat tggaagcggg aaagcctgaa
     2221 tggcgtcaaa tcaaaatcgc tttttttgtc ttgcgcaaaa tcggcagcta tttcccctaa
     2281 aactgacgca aatttaaaac cgtgcccact caggccggta atgagcagtg tattatcgtg
     2341 gccgggtagg gtatcgataa taaaatcttc gtcaggcgaa ttatcatagg tgcaggcagc
     2401 gccgtacagg cagcaaccga tacccggcaa tacattgcgc aagaacggga aggcttccga
     2461 accatcgctg accacttccg caaacggaac acgttcatcc gctgaatgga taacctggcc
     2521 tccgttatgt ttgccaatct tcaacgcgtc gttttctgcc ggaaaaccat aatattgatc
     2581 gccattgggc agttcaccgg taaacgccgg gaatttattc ttcacgctat agcggccatc
     2641 ggcctgatac caggcaaata ctttacgtac aggctggaca ggcagctccg ggagcaggtc
     2701 ttttacccat gttcccgcgc agacaatcgc ttttttcgcc tgatactcac cgtcaacggt
     2761 ttcaatagtt acgccatcat cgtcatgacg aattgcggtg accgggcagt tgaacagttg
     2821 cgcacagccc gcttccttcg ccagttggat ccaggtttta atcgccagtt cgctgcgcaa
     2881 aaaaccggaa tcagtctcaa ataagccgat gtagttgtcc gggacgcgta tttctggcca
     2941 gcgggccata atcccttgcg catcgagctt ttcaacgttg agttgccact gttcggcgct
     3001 gtgggcgacg ttggcgagaa atgcggagtc agccgggccg aggttaatga caccagagcg
     3061 tacaaaaatg ggatcatctt cgttgtggcg ggaaagttca tcccacagcg tttgcgcgcg
     3121 gaggaccaac gggacatact tttcgccttc accataagca tggcgaatta atcgcgtatc
     3181 gccgtggtgg ctgccgtgtt gatgcggtgg catatgggcg tcggtcatta gcacgtttaa
     3241 accggagcgg gttgcataat acccggcggc agcgcctacg gaaccgctgc caataatgat
     3301 gagatcgtat ttcatttttt tctctctgct ctcatggttt aagcagagta attaactgaa
     3361 tgcagatata caacccagaa atggtaaagg caccggtgag gtgccttttg ggtggatggt
     3421 catgtcatgt taatgacgcc gatactcgtt taccgggaaa tcaccggatt caattttggc
     3481 gattcctgct tctaatatcg aaataaattg tctggcaaca tcagtggtta accagagcgt
     3541 ctgaccaact tcagtccctt cctgctcgga cttatttggg gtctggtagt gcaaacgcaa
     3601 catcagcgca tcatagctat caacggtgct gatgtcccac cctacgagcg gatgagtctg
     3661 aatgacttca ttattttttt ccatcatggc cccctaattc gtgttactag acaacgttat
     3721 tcgaggttca atgcgttttt atctgaagca acttcagtat accaataagt aagggtattc
     3781 accgaatttt taaagggggg aacagataaa tttctgcttt taagatttat atgaacaata
     3841 aaacagcatg tcattcatat tttttagcat attgtgcaat tattttgagg aagtgtagaa
     3901 attttgtact caaaatttgt aagtaaaata aaaaagccgg ggcgacccgg caaaaacaaa
     3961 aatcactgca tattattcgc tgacaaacca gtcatcggcg ctttcccacg tttcctggag
     4021 aatttcgcta atgcgctgtt tatcttcttt tgttacgcca ataaccgata aattattcgc
     4081 tgcggcataa cgtaccgata cgtggccttc attatcagga aacgcatact gaatacggcg
     4141 ggaaagttcg ccagccaggg cgtcaatagc cccagctggc aatggagaag ttttcgctat
     4201 ggtgacttca attcgcataa tagccccctg ttgaatatac tggttattta tacaggcaaa
     4261 ataacctaat gacaacagga agctacgatt tttattgttt aacggaccag cgtaccgttt
     4321 ccccggcgag gaatggcacc agggtgtcat cagtcagtgc gatgctttca gcaacctgtt
     4381 gctcttcacg taccagttcg atgaatgtgt cgttgaccgg caagccatag aactgcgggc
     4441 cgtttacaga acagaatgct tcaaagtgct gcagagcatt catctcttca aagacggtag
     4501 cgtaactgcc cagcgcggtt ggggcgttga agcagcccgc gcagccgcaa ctgctctctt
     4561 tgcgatgacg tgcatgtggc gcagaatccg taccgaggaa tactcgatta aaaccgctgg
     4621 cgaccagttc acgcaatgcc tgttggtgaa tattgcgttt gaggatgggt aaacaataca
     4681 ggtgcggacg cacgccacca accagcatat ggttgcggtt aaacatcaga tgctgcggag
     4741 tgatggtggc agccagccgt tcatttccgt cacggacata gtcggcagca tctttggtgg
     4801 tgatgtgctc aaaaacgact ttcagcgcag tcaggcgctg gcgcagaggt tccatcacgc
     4861 tttctataaa gtgcgcttca cgatcaaaaa tgtcgatatc tgcatgtgtc acttcaccat
     4921 gcaccagtag cggcataccg attttttcca tgcgctcaag taccggcatg attgcgtcaa
     4981 ttgacgtcac gccgtggctg gagttagtgg ttgcgtttgc cggataaagt tttgcagcgg
     5041 tgaacacgcc ttcgttaaat ccgcgctcca gctcattagg atccagcgaa tctgttaaat
     5101 aacaggtcat caatggggtg aaattgtgcc cggcaggtac ggcatcaaga atacgctggc
     5161 gatacgccac ggcagcctca acagtggtca cgggcggagc cagattgggc attacaatag
     5221 cccgtccata aatttcgctg gtatacggca cgacagtttt taacatgtcg ccatcgcgga
     5281 ggtgaaggtg ccagtcgtct gggcggcgga tctttaatac ctgggatggt gcagtcatta
     5341 atctctatgc tccggctgaa gggatgtttt tgccggacac aaaggataag cggaaacgtt
     5401 ttcctttgca cgaaaaataa agggcgcgaa tgcgccctag tcattaatcc gtaaacggaa
     5461 tgacaatttc gcctggcttc acttcaatgc ctttcgccag ttttttcgcc attgcttcgc
     5521 cctgactgcc atcttcgcgc aggacgtaag caggttgctg gttaaagtaa ttgcgtaatg
     5581 cctggttcaa atagggaagc aacgtttgca tcaccgtttg cattttttcc ggttgtaccg
     5641 tcgcatcgac cacttccatc tctttcagga agatcgcacc tttttcttta tcaaacacgg
     5701 gcagcgcttt cagcttcagt ttcatggtcg ctttctgact accgaacagg gagttcatgt
     5761 ccagattggc gtctccggtt agggtaacct tattcggctc ttcgcgacca atttggctgg
     5821 tcaagtttgt cagaacaata tgggcgtcag ccacgccggg taaaccgata tcttttgaga
     5881 aattattatg tttcgcaagc gactggttaa tttcttgttc ggtgatggtg tattgggtga
     5941 gttgattaca gccaacgagc aggccactga cgatcaatgc agcggcaaat aaaaacttgt
     6001 tcatggtagt cctcgacatg aaatctgcgt caatatcctg acacaacgca gcatgtgtca
     6061 ccagcgataa actcgccagc agaaaaaact gaaaacggcg gcaacccgcg aatacaggct
     6121 gccgcggcgg gtcaggatta aatcgccatt gatgataaca aattgatttg tgtctgtttc
     6181 gccatattat cgcggtaatc agcaacgcgg cttggccagt tgattccggc taccagcgtc
     6241 agattacgca gtagcggaaa tagctgaata tcatcttccg aaagttcgcc attcacggcg
     6301 ttcggtttga cgatcagttt gtccagcgca cgcaaatcat cgctgatatt cttaatcaga
     6361 ccgtcagagt gggccagcag gtcggcaaaa ttacccgcgc tggcctcttt cttgtcgacg
     6421 aaatatttgc gcgcggcggg agtagaaaac tcatcaaatg ccgatttggc aaaacgcggc
     6481 aacagcagtt tgttggcgta gccattgacc ttgcgcagcc actcttcaat ggcaggggaa
     6541 cgtttgccgg tcagtaacgg tttgccgtcg agtttatcga catagtgaac gatatccatg
     6601 ctttctggca tatagcggct gtcatctttt tgcagaatgg gaacctgttt ttgaccgacc
     6661 atccgggtgg gtgtttctgc gtcgtcgttg agcagaacat gtaattcgac ggggatattt
     6721 ttcaggccga aaatcatgcg ggctttgagg cagtaagggc agtgatcgta aatgtatagc
     6781 ttcacgtgac tcctccattt ggctgtcagt tcactttcag tatggaggag tcagggggga
     6841 gtatcaaatc aggcgtcgcg ttccagcaaa cgacgcgcgg cgcgtttctg actaaactgc
     6901 caacccagcg caaggaaagt gaagatgcca ataattccca gcatcatcca cggaagctct
     6961 ggctggtgcg ccgatttgcc caggtcaaac agccagccac caccgatata accaatagcg
     7021 ccgccaatcg ccagacccag acggctaaac cccatatagc tgccgcgagc tcttgcgtcc
     7081 gccagcgaag cacttaaggt ttcacgcgca ggctcggcaa tgatcgactc gatataaaac
     7141 agacaaatca gggtgaaaag ttgttgcagg ccgctgacca tgcccaccgg catcatgctt
     7201 aatgacatta tcaacagccc agccatcaac cggtgttcca gacgaaaatg cttttcactc
     7261 cagcgggcga tagggtagag caacgttaac gacagacacg cttcaatggc atacatccat
     7321 ttaacggcag agggcgcgcc agccacgtcg ttgaccataa ttggcagcat cagcatcact
     7381 tgtacagcca gcatgtagta acccgccagc gtcaggacat aggtgacaaa acgcttgtca
     7441 cgcatcacgc gggtcatgcc ttcgcgaacg ggcgtgcgta cggtggagag tttccatgcc
     7501 ggtaacaacc acgcattgaa cgccgcacac agcacaaaca aaacggcccc tgtggcgcag
     7561 accaggcgaa agtcgtattg caacagccag ctccccaaca atgcgccaat gaccgcaccg
     7621 gcactgtcct gcatcatcaa cagcgagaaa aaacgaccac gttgttgtgg acggattagt
     7681 ttcaccacca gcgccgaacg cggcggatca aacaacgtgc cgccgagtcc cgagagcagg
     7741 catgaaaacc acaatagcca cggttcgtgg gcgataccca ttgtggcgaa tccggcggcg
     7801 cgcatcagca taccggtaac aatcatcggt ttggcaccaa agcggtcggc aattgcgccg
     7861 ccgaaaatac ccagaccttg ctgaataaac tggcgtagac cgagagcaat accgaccatg
     7921 acggcggccc agcccatttg atcaacgaag cggatagaga tcagcgggaa gacaacaaag
     7981 aaccccagca cgaccagcat attatcgatg agcaggaaat atttacccag gttcctcgcc
     8041 tgcgatacgc gggacatttc ccctcccggg aaataaaaga tgagcacttt ctattctgca
     8101 ttgccgctaa gacttttccc atcacttcgg ggacaatatt ttttatcaaa acgtcgcttt
     8161 gatagagagt ttttatcaaa tatgtgaata atgcagaaaa tggcattttg gacttttcac
     8221 agggtctggt tgcgcaggta tagtaatact tacagcgtat taaagacgtt acgggaagga
     8281 gtaggtatag aatgtttggc tatcgcagta acgtgccaaa agtgcgctta accacagacc
     8341 gactggtcgt gcgtctggtg catgatcgtg atgcctggcg tcttgcggat tattacgcag
     8401 agaatcgcca tttcctcaag ccctgggagc cagtgcgcga cgaaagccac tgttatccat
     8461 caggctggca ggccaggctg gggatgatta acgaatttca taaacaaggt tcagctttct
     8521 actttggctt attcgacccg gacgaaaaag agattattgg cgttgccaat tttaccaatg
     8581 ttgttcgtgg ctcttttcat gcctgctatc tcggttattc gattgggcaa aaatggcagg
     8641 gcaaagggct catgtttgaa gccctggccg cagccattcg ttatatgcag cgcacccaac
     8701 atattcatcg cattatggct aattatatgc cgcacaataa acgcagcggt gatttactgg
     8761 cgcgactggg ttttgaaaaa gaaggctatg cgaaagacta tctgttgatt gatggacaat
     8821 ggcgcgatca cgtactgacg gcattaacta ccctagactg gacgcccggc cgctaaggag
     8881 aatctcatga aatatcaact tacagccctt gaagctcgcg tgattggttg cttgctggaa
     8941 aagcaggtga ccacgcagga gcaatacccg ctctcagtca atggtgtagt cacggcctgt
     9001 aatcagaaaa cgaaccgtga accggtcatg aatctgagtg aatctgaagt gcaggagcaa
     9061 ctggataatc tggtcaaacg ccattatctg cgcacggtga gtggctttgg taatcgggtc
     9121 accaaatatg aacaacgttt ttgtaattca gaatttggcg atctgaaact gagcgcagcg
     9181 gaagtggcgt taatcaccac gttgttattg cgtggtgccc agacgccggg tgaactgcgc
     9241 agccgcgccg cgcgaatgta tgaattcagc gatatggcgg aagtggagtc gacgctggaa
     9301 caactggcaa atcgcgaaga tggtcctttt gtggtgcgtc tggcccgcga accgggtaaa
     9361 cgcgaaagcc gctacatgca tcttttcagt ggtgaggtag aggatcagcc ggcggtaacg
     9421 gatatgtcga acgcggttga tggtgattta caggcccgcg tcgaagccct ggaaatcgaa
     9481 gtggcagaac tgaaacagcg tcttgattcg ttgctggccc atctgggaga ttaaagtgaa
     9541 aaaattacgt atcggcgttg tgggattagg cggcattgcg caaaaagcgt ggttaccggt
     9601 gctggcggca gcgtctgact ggatgttaca aggagcctgg tcgcctacgc gcgcgaaagc
     9661 cctgccaatt tgtgaaagct ggcgcattcc ttatgccgat tcgttatcca gccttgccgc
     9721 cagttgcgat gcggtttttg tgcattccag caccgccagc cactttgacg tggtcagtac
     9781 gttactcaat gcgggtgtac atgtctgtgt cgataaaccg ctggcagaaa atctgcgcga
     9841 tgctgaacgg ctggtggaac tggcggcgcg gaaaaaactg acgttgatgg tcggttttaa
     9901 ccgtcgtttc gcaccactct acggtgagtt aaaaacgcaa ctcgctaccg catcttcgct
     9961 aagaatggat aaacatcgta gcaatagtgt cgggccacac gatctttatt tcacgttgct
    10021 ggatgattat ctgcatgtgg tggataccgc gctgtggttg tcgggcggca aagcctctct
    10081 ggatggcggt acgctactga ctaacgacgc tggcgaaatg ctgtttgccg agcaccattt
    10141 ttcggccggt cctttgcaga tcaccacctg tatgcatcgc cgtgccggaa gtcagcgtga
    10201 aaccgtgcag gccgtgactg acggtgcgct catcgacatt acggatatgc gcgaatggcg
    10261 tgaggagcgc gggcagggcg tagtgcataa accgattcct ggttggcaga gcactcttga
    10321 acaacgtgga tttgtcggct gtgcgcggca cttcattgaa tgtgtgcaaa atcagacagt
    10381 tccgcaaacc gccggcgaat aggccgtgct ggcgcaacgt atcgttgaca agatctggcg
    10441 cgatgcgatg agtgaataaa ccctgtaaca tctggcggta gcaattcacc ataatccagg
    10501 tagactattc gcctctttca gcgcctgtct tgcaggcgtt ttgcccgttg gtctggatta
    10561 gaacaccgat gaatttatta aaatcgctgg ccgccgtcag ctcgatgacc atgttttcgc
    10621 gtgtgcttgg cttcgcacga gacgcaattg tcgccagaat ctttggcgca gggatggcaa
    10681 ccgacgcctt tttcgtcgct tttaaacttc ctaacttgtt acgccgtatc tttgccgaag
    10741 gggcattttc ccaggcattt gtaccgattc tggcggaata taaaagtaag cagggtgaag
    10801 acgccacgcg ggtctttgtc tcttatgttt ctggcctgct gacacttgcg ctggcggttg
    10861 tgacggtcgc tggcatgctc gccgcaccgt gggtgatcat ggtgaccgcg ccaggcttcg
    10921 ctgacacagc tgacaaattt gccctgacca gccagctact aaagattacc tttccctata
    10981 tcttgctgat ctccctggcg tcgctggtgg gagcgattct gaatacgtgg aaccgcttct
    11041 cgattccggc gtttgcgcca acactgctta acatcagcat gattggtttc gcgctgtttg
    11101 ccgcaccgta ctttaaccca ccggtgctgg cgctggcgtg ggctgttacg gtcggcggca
    11161 tcctgcagct ggtgtatcag ctaccgcacc tgaagaagat cggcatgctg gtcctgccgc
    11221 gcattaactt ccacgatgcc ggagcaatgc gcgtggtgaa acagatggga ccggcgatcc
    11281 ttggcgtctc tgtgagccag atctccttaa tcatcaacac catttttgcc tcgtttcttg
    11341 cttccggttc ggtgtcttgg atgtattacg ccgaccgctt aatggagttt ccgtccggtg
    11401 tgctgggcgt ggcgcttggc accattttgc tgccgtcgct gtcgaaaagt tttgccagtg
    11461 gcaatcatga tgaatacaac cgtttgatgg actgggggtt gcgtctttgt ttcctgttgg
    11521 cgctgccgag tgcggttgcg ttgggcattc tttcaggtcc gttaaccgtt tcgctgttcc
    11581 agtacggtaa atttaccgcg tttgatgcgc tgatgaccca gcgggcgtta attgcctact
    11641 cggtgggttt gattggcctg attgtagtga aagtgttggc tcctggcttt tattcccgcc
    11701 aggacattaa aacgccagtg aaaattgcca tcgttacgct gatgttaacg caattgatga
    11761 acctggcgtt tattggtccg ttgaaacatg ccgggctgtc actttctatt ggtctggcgg
    11821 cgtgtctgaa tgcttcgctg ctttactggc agttgcgtaa gcagaaaatc tttactccgc
    11881 aacccggctg gatggcgttt ctgttgcgtc tggtggtggc ggtactggtg atgtcttgcg
    11941 tgcttttagg tatgttacat atcatgccgg agtggtcatt gggtaccatg ccctggcgct
    12001 tactgcgttt aatggcggtc gtgctggcgg ggattgccgc gtacttcgct gcactggcgg
    12061 tactgggctt caaagttaaa gaatttgccc gccggacggt gtaacaatgc attccggcct
    12121 gcagtgcagg ccggagataa tcttcagatc gaaatctttt taccgccgcg atgggttgtt
    12181 gaggtttgac cgttcgcccc atatagcgtc ggttcctgat gcggtttcaa catttccagc
    12241 gcctgttgat tgcgctcaat ctgtccttcc agtaaccaac cgttatgttg attcatttgg
    12301 cgtagttgct gcgttttcac agtaatttcc tgccagcgtt gactaatatc aacgctattt
    12361 gctgtattgg gttctttctt gcgtaactgt tcgaggtaat ccagcgtcgc cagcagtgag
    12421 cttttttgtt ctgtaatcca ttgcaactgg ctgccgttga tctgccccat agagagatgt
    12481 tgctgctctt gatccattac cgttttgaga tcgttaagca cagcggacat ctggtcaagg
    12541 atctctgcaa gacgtgtcat acgatcagtt actctgcaag tcttgctgag cttcgttgat
    12601 cagcgcatcg gcaattttgc cgatgtccat ttttagttca ccgttacgaa tcgccagttt
    12661 taacgcttcg acacgttcaa gattgatatc actgctgccg ggttgcatca gttttgcttg
    12721 cgcgtcgctt aacgtcacac tggtgctggt gctggtggag tcggttgttt ttgccgcccg
    12781 ggtgttcgtt accggcgcgt cagtggtttc gcgcggttga acggtgctta cgggattcag
    12841 aggcgaagtg cgatcaatac tcatggttta ttcctcattg agggcgcttt tatcatgtgt
    12901 tgcttattta tcggcaaggg acgggtaatc tttaacagct tacaggttta taagaatatt
    12961 cccatctgca tcaacaacgc cgctgaccac cagtcccgat accatgcgca cccgcgcatt
    13021 ctgtgcgacg gctgcattgt tcagcgcctg accttctgcg ttggcgctaa acccgtcacc
    13081 gctggcgatc acgttgacgc gttgtcccgc ttttacccgc catgcctggc gaaaatgggt
    13141 taattggatt ggttgatcgg gtgatagatc gcgtaggcta atggcatcaa caagttgatt
    13201 gatatccagc accgtacgcg gtggcagggt atccagtcgt ccgcgtttta gtttgacatt
    13261 gccagcttcc agctttcctc cccgcacaat gggcatcgcg gcaaccacat aatttcctgt
    13321 ggcctgcaca ttaacctgta aatatcgttt gtcgttaccg cagcgtgcca acacattcac
    13381 attgccccac aggcgggaat tattgctcat cgaaagcaat ggctgctcgc acggcggtag
    13441 tagattgggt gctgtacgaa tagaaacacg aacctcatca cttaccccca cgagctgggc
    13501 gctaaaaaag gtgtgcaatt gcgatgtgag attgctcgcc gcacttaacg gactgaacag
    13561 tatcgcgatg atcgccacgc tacgttttat tgccagcatt ttcgcccccc agctatttct
    13621 acaacgtgaa ttgtacccgt ccccaatggt catcaacggc ataaatagcg acccattttg
    13681 cgtttattcc gccgataacg cgcgcgtaaa ggcatttaag ctgatggcag aattttgata
    13741 cctgcggagg agatatgctc gataagctcg acgccgcctt acgttttcaa caagaggcgc
    13801 tcaatctgcg cgcccagcgt caggaagtgc tggcagcaaa catcgccaat gccgataccc
    13861 ctggttatca ggcgcgcgat atcgattttg ccagtgaact taaaaaagtc atgcaacgtg
    13921 gacgggatgc aaccagtgtg gttgctctga cgatgacctc aacgcaacac attccggcgc
    13981 aggcgctgac gcctcctacc gcagaactgc aataccgcat tccggaccag ccttcgcttg
    14041 acggtaatac cgtcgatatg gatcgcgaac gcacccagtt tgccgataac agcctgcaat
    14101 accagatgag ccttagtgcg ttgagcgggc aaatcaaagg catgatgaac gttttacaga
    14161 gcggaaatta acggatggca ctgctgaata tttttgatat cgccgggtcg gcgttaactg
    14221 cccagttcca gcgcctgaac gtggcggcca gtaatctggc gaatgctgat agcgtgaccg
    14281 gtcccgatgg acagccatat cgggcaaaac aggtggtatt ccaggttaac gctgcaccag
    14341 gtgctgcgac aggcggcgta aaggttgccg atgttataga aagtcaggcc ccggacaaac
    14401 tggtttatga accgggtaat ccgctggcgg atgcaaaggg ctacgtaaaa atgccgaacg
    14461 ttgatgttgt cggagagatg gttaacacca tgtcggcgtc acgcagctat caggccaatg
    14521 ttgaagtgct caacacggtg aaaagcatga tgctgaaaac ccttacgctc ggtcaataaa
    14581 ggagaaagct atgtccattg cggtaaccac caccgatccg acaaataccg gcgtcagtac
    14641 caccagcagt agttcgctca cgggcagcaa cgccgcagat ttacaaagca gttttctgac
    14701 tttgctggtg gcgcagctga aaaactagga cccgaccaat ccaatggaaa acaacgagct
    14761 gacgtcgcaa ttggcacaaa tcagcacggt cagcgggatt gaaaaactca ataccacgct
    14821 cggatctatt tccggacaga ttgataacag ccagtcgtta caggccagta acctgatcgg
    14881 tcacggcgtg atgatccccg gcacaattgt tcttgcggga accggcagtg aagaaggggc
    14941 tgtgaccacg accacgccgt ttggtgttga gctgcaacag gcggcagaca aagttacggc
    15001 caccatcacc gataaaaatg gcgcggttgt gcgcaccatt gatattggtg aactgaccgc
    15061 cggagttcac agtttcacct gggacggtac gttgactgat ggcagcactg cgccgaacgg
    15121 ttcttacaat gtagcgatta ccgccagtaa cggtggtaca caactggttg cccagccgct
    15181 gcagtttgct ctggtgcagg gtgtgatccg cggcaacagc ggtaatacgc tggatctcgg
    15241 cacttacggc accaccaccc tcgacgaagt acggcagata atttaagcct tcacatttca
    15301 ggagtcagtc atggcctttt ctcaagcggt tagcggatta aacgctgccg ccactaacct
    15361 cgatgttatt ggcaacaata tcgccaactc cgccacctac ggctttaaat caggcacggc
    15421 ctcttttgcc gatatgtttg ccggttcgaa agtgggactg ggggtaaaag ttgccggtat
    15481 cactcaggac tttacagatg gcacgaccac caacaccggg cgaggtctgg acgttgctat
    15541 cagccagaac ggttttttcc gtctggtaga cagcaacggt tcggtgttct acagccgtaa
    15601 cggacaattt aagctggatg aaaaccgtaa cctggtgaat atgcaaggtt tacagctgac
    15661 gggttacccg gcaaccggta cgccgccgac tattcagcaa ggggcgaatc cgaccaatat
    15721 ttcgatcccg aataccctga tggcagcgaa aactaccacc acggcgtcga tgcagatcaa
    15781 cctgaattcc agtgatccgc ttcctactgt tacgccattc agcgccagca atgcggatag
    15841 ctataacaaa aaaggttcgg tgactgtttt cgacagtcag ggtaatgctc atgacatgag
    15901 cgtctacttt gtgaagaccg gggataataa ctggcaggtc tacacccagg atagcagtga
    15961 tccaaacagc attgcgaaga cagcgacaac actggaattt aatgctaatg gcacattagt
    16021 gaatggtgcg atggcgaata atatcgcaac cggcgcaatt aacggtgcag aacccgccac
    16081 gtttagtctg agcttcctca actccatgca gcaaaatacc ggcgctaaca atattgtggc
    16141 aaccccccag aacggctaca aaccgggcga tctggtgagt tatcaaatca atgatgacgg
    16201 tacggttgtc ggcaactatt ccaacgaaca aactcaactg ctggggcaga ttgtactggc
    16261 gaactttgcc aacaacgaag gtctggcatc cgaaggcgac aacgtctggt ctgcgacgca
    16321 atcttctggc gtggcgctgt tggggacagc cgggacggga aactttgcca ccctgaccaa
    16381 cggtgcgctg gaagcgtcca acgtcgatct cagtaaagaa ctggtcaata tgatcgttgc
    16441 ccagcgtaac tatcagtcta acgcccagac catcaaaacc caggaccaga tcctcaaaac
    16501 gctggttaac ttacgctaat cgctgacggg atagctcaat ggatcacgca atttatactg
    16561 cgatgggagc agccagccag acgcggaatc aacaggcggt aaccgccagt aatctggcca
    16621 atgcctctac gcccggtttt cgcgcgcagt taaacgcctt gcgcgcagtg ccggtggaag
    16681 ggctttcact gcccacgcgc acgctggtaa cggcgtcaac accgggtgca gacatgacgc
    16741 ccggcaagat agattacacc tcgcgcccgc tggacgttgc gttgcagcag gatggctggc
    16801 tggccgtgca gaccgctgac ggcagcgaag ggtatacgcg taatggcagc attcaggttg
    16861 atcccaccgg gcaactgaca attcaggggc atcaggtgat aggcgaggcg gggccaattg
    16921 ctgtgccgga aggagcggaa atcactattg ctgccgatgg cacaatctcg gcgctcaatc
    16981 cgggcgatcc ggcaaataca gttgcgccag tagggtgtct gaaactggtg aaagccacgg
    17041 gcagcgaagt gcagcgcggt gacgacggca tttttcgttt aagcgcagaa acccaggcca
    17101 cgcgagggcc ggtactgcag gcagatccaa ccttgcgtgt gatgtcgggg gttctggaag
    17161 gcagtaacgt caatgccgtt gcggcaatga gcgacatgat tgccagcgcg cggcgttttg
    17221 aaatgcagat gaaggtgatc agcagcgtcg atgataacgc aggccgtgcc aaccaactgc
    17281 tgtcgatgag ttaattgaac gattgatgac attgcaataa aagcgcgcgg actattccct
    17341 cgcccctttg gggagagggt gagggcgagg ggaaattcac actgtcgcgt gcttctgctc
    17401 tgcttgtcac taatccacta caggacattt tatgatcagt tcattatgga tcgccaaaac
    17461 gggccttgac gcccagcaaa ccaatatgga cgtcattgcc aacaacctgg caaacgtcag
    17521 tactaacggt tttaagcgtc agcgcgcggt gtttgaagat ctgctttatc aaaccattcg
    17581 ccagccgggg gcacagtctt ccgaacaaac caccttaccc tccggattac aaatcggcac
    17641 gggggtacgc ccggtcgcca ctgaacgctt acacagccag ggaaacctgt cgcagaccaa
    17701 caacagcaaa gatgtcgcga ttaaagggca gggctttttc caggtgatgt tgccagatgg
    17761 ttcatcagcc tatacccgtg acggctcttt ccaggtggat cagaacgggc agctggtgac
    17821 ggctggtggt tttcaggtgc agccagcgat caccattccg gcgaatgcgt taagtatcac
    17881 catcggtcgt gatggcgtgg tcagcgtaac ccaacaaggc caggcagctc cggttcaggt
    17941 tgggcagctc aatctcacca cctttatgaa tgacaccggg ctggagagca ttggtgaaaa
    18001 cctctacacc gaaacgcaat cctctggtgc accgaacgaa agcacgccgg gcctgaacgg
    18061 cgcgggattg ctgtatcaag ggtatgttga aacgtctaac gtcaacgtgg cggaagaact
    18121 ggtcaatatg attcaggtgc aacgcgctta cgaaatcaac agtaaagcgg tgtccaccac
    18181 cgatcagatg ctgcaaaaac tgacgcaact ctaaggctta accggtggca ggttcaccgg
    18241 tttactgatt tttgaagatg atagccatgc aaaaaaacgc tgcgcatact tatgccattt
    18301 ccagcttgtt ggtgctttca ctaaccggct gcgcctggat accctccacg ccgctggtgc
    18361 agggggcgac cagtgcacaa ccggttcccg gtccgacgcc cgtcgccaac ggttctattt
    18421 tccagtctgc tcagccgatt aactatggct atcaaccgct gtttgaagat cgtcgaccac
    18481 gcaatattgg cgatacgctg accatcgtgt tgcaggagaa cgtcagcgcc agcaaaagct
    18541 cctctgcgaa tgccagccgt gacggtaaaa ctaattttgg ctttgatact gtgccgcgct
    18601 atttgcaggg gctgtttggt aacgttcgtg ccgatgtcga agcctccggt ggtaacacgt
    18661 tcaacggaaa gggcggggcc aatgccagca atacctttag cggcacgttg acggtgacgg
    18721 ttgaccaggt actggtcaac ggcaacctgc atgtggtggg tgaaaaacag attgccatta
    18781 atcagggtac cgaatttatt cgcttctctg gcgtggttaa tccacgcact atcagcggca
    18841 gcaataccgt accgtctact caggtggtgg atgcgcgcat tgaatacgta ggcaatggct
    18901 acattaacga agcgcaaaat atgggctggt tgcagcgttt cttccttaac ctgtcgccaa
    18961 tgtaagtgag gctgtggtga ttaaatttct ctctgcatta attcttctac tggtcacgac
    19021 ggcggctcag gctgagcgta ttcgcgatct caccagtgtt cagggggtaa ggcaaaactc
    19081 actgattggc tatggtctgg tggtggggct ggatggcacc ggtgaccaga caacccagac
    19141 gccgtttacc acacaaacgc ttaataacat gctctcacag ctgggaatta ccgttccgac
    19201 gggcaccaat atgcagctaa aaaacgtcgc tgcggtaatg gtgacagcgt cacttcctcc
    19261 gtttggacgt caggggcaaa ccatcgatgt ggtggtttct tccatgggta atgccaaaag
    19321 cttgcgtgga ggtacgttgt tgatgacacc gcttaagggc gttgacagtc aggtgtatgc
    19381 gctggcgcag ggcaatattc tggttggcgg cgcaggagcc tccgctggcg gtagcagtgt
    19441 tcaggttaac caactgaacg gtggacggat caccaatggt gcggttattg aacgtgaatt
    19501 gcccagccag tttggcgtcg ggaataccct taatttgcaa cttaacgacg aagatttcag
    19561 catggcgcag caaatcgctg acaccatcaa ccgcgtgcgt ggatatggca gcgccaccgc
    19621 gttagatgcg cggactattc aggtgcgcgt accgagtggc aacagttccc aggtccgctt
    19681 ccttgccgat atccagaata tgcaggttaa tgtcaccccg caggacgcta aagtagtgat
    19741 taactcgcgc accggttcgg tggtgatgaa tcgcgaagtg accctcgaca gctgcgcggt
    19801 agcgcagggg aatctctcag taacagttaa tcgtcaggcc aatgtcagcc agccagatac
    19861 accgtttggt ggtggacaga ctgtggttac tccacaaacg cagatcgatt tacgccagag
    19921 cggcggttcg ctgcaaagcg tacgttccag cgccaggctc aataacgtgg tgcgcgcgct
    19981 caatgcgctg ggcgctacgc cgatggatct gatgtccata ctgcaatcaa tgcaaagtgc
    20041 gggatgtctg cgggcaaaac tggaaatcat ctgatgatca gcgacagcaa actactggca
    20101 agtgcggcct gggatgcgca atcactcaac gaactaaagg cgaaagcggg cgaagatccg
    20161 gcggcaaata tccgtccggt ggcccgtcag gtggaaggga tgttcgtgca gatgatgttg
    20221 aaaagcatgc gcgacgcttt accaaaagat ggcctgttca gcagcgagca cacttgcctg
    20281 tataccagta tgtatgacca gcagattgcc caacagatga cggcgggcaa aggtctgggg
    20341 cttgcagaga tgatggttaa acagatgacg ccagaacaac cattgccaga ggagtccacg
    20401 ccagcagcac cgatgaaatt cccgctcgaa actgtggtgc gttatcaaaa tcaggcgttt
    20461 tcgcagctgg tgcaaaaggc cgtgccacgt aactacgatg attcgctgcc gggtgacagt
    20521 aaagcattcc tcgcgcaact ctcgctgccc gcccaactgg caagccagca aagcggtgtg
    20581 ccacatcatt tgatcctcgc tcaggcggcg ctggaatctg gttgggggca acggcaaatc
    20641 cgccgcgaaa acggcgagcc gagctataac ctgtttggtg tcaaagcctc tggcaactgg
    20701 aaagggccag ttactgaaat caccacgact gaatatgaaa acggcgaagc gaagaaagta
    20761 aaagcgaagt ttcgggtcta cagctcgtat ctggaagcat agtcggatta cgttgggctg
    20821 ttaacgcgta acccgcgcta cgccgccgtg accaccgcca cgagtgcgga gcagggggcg
    20881 caggccctac aggacgcggg ctatgccacc gatcctcact atgcccgcaa actcaccaac
    20941 atgattcagc agatgaaatc gataagcgac aaggtgagca aaacctacag tatgaacatt
    21001 gataatctgt tctgaataac tcaagtccgg cgggtcgctg ccgataatac tctgtaattg
    21061 aaggcttata aggaacctcc atgtccagct tgattaataa cgccatgagc ggactgaacg
    21121 cggcccaggc ggcgttaaat acggcaagta ataatatctc cagctataac gttgccggat
    21181 atacccgcca aaccactatt atggcgcagg ccaatagcac gttgggcgct ggcggctggg
    21241 ttggcaatgg tgtctacgtt tctggtgtgc agcgtgagta tgatgcgttt atcaccaacc
    21301 agttacgtgc ggcgcagacg caaagtagcg gcctgactgc ccgctatgag cagatgtcga
    21361 aaatcgacaa tatgctctcc accagtacct cttcgctggc aacacagatg caggatttct
    21421 tcaccagcct gcaaacgctg gtgagtaacg cggaagaccc ggcagcgcgc caggcgctga
    21481 ttgggaaatc agaagggttg gtgaatcagt ttaaaaccac cgatcagtat cttcgcgacc
    21541 aggacaaaca ggtcaatatc gcgataggtg ccagcgttga tcagatcaac aattacgcta
    21601 aacaaatcgc cagcctgaac gaccaaatct cccgcctgac aggcgtgggg gcaggggcgt
    21661 cacctaacaa tctgctggat caacgcgatc aactggtgag cgaattaaac cagattgttg
    21721 gtgtagaagt cagcgttcag gatggcggca cttataacat cacgatggcc aatggttact
    21781 cactggttca gggaagtacg gcgcggcaac tggcggcagt tccttccagc gccgaccctt
    21841 ctcgtacgac tgtcgcttac gttgatggga cggcaggcaa tattgagatc ccggagaaat
    21901 tactgaatac cgggtcgctg ggcggcattc tgacattccg ttctcaggat ctggaccaga
    21961 cgcgtaatac gcttggacaa ctggcgctgg catttgccga ggctttcaac acccaacaca
    22021 aagccggatt tgatgccaac ggcgatgctg gtgaagattt ctttgctatc ggtaagcccg
    22081 cggttttgca aaacacgaaa aacaaaggtg acgttgcgat cggtgccacg gtaactgatg
    22141 cctccgcggt actggcgaca gattacaaaa tctcgttcga taataatcag tggcaggtca
    22201 cccgccttgc cagcaatacc acttttacgg tgacgccgga tgccaacggt aaagtggcat
    22261 ttgatggtct ggagttgacg tttacaggaa cgcctgccgt taacgacagc ttcacgctga
    22321 aaccagtaag tgacgccatc gtcaacatgg atgtattaat caccgacgaa gcgaaaatag
    22381 cgatggcgag cgaaaaagat gcgggtgata gcgataaccg caacggtcag gccctgctgg
    22441 atctgcaaag caacagtaaa acggtgggcg gtgcgaaatc ctttaacgac gcttatgcct
    22501 cgttagtgag tgatatcggt aataaaaccg cgacgttgaa aaccagtagc gccacgcaag
    22561 gtaatgtggt gacgcagctt tccaatcagc agcagtctat ctctggcgtc aatctcgacg
    22621 aagagtacgg aaatctgcaa cgttttcagc agtattacct ggcgaatgcg caggttctgc
    22681 agacggcaaa cgcgattttt gatgcgctga ttaacattcg ctaaggggag ataagatgcg
    22741 tttcagtacc cagatgatgt accagcaaaa catgcgtggc atcaccaatt ctcaggcgga
    22801 atggatgaag tacggcgagc agatgtcgac aggtaagcga gtcgttaacc cttctgacga
    22861 tcccattgca gcatcacaag ccgtagttct ctcccaggca caggcgcaaa acagccagta
    22921 cacgctggcg cgtactttcg ccactcaaaa agtgtcactg gaagagagtg tacttagcca
    22981 ggtcaccact gctatccaga atgctcagga aaaaattgtc tacgccagca atggcacctt
    23041 gagtgacgat gaccgggcct cgctggctac ggatattcag gggcttcgtg accagttgct
    23101 gaatctggcg aacaccactg acggtaacgg gcgctacatt tttgccggtt ataaaacaga
    23161 gactgcgccg tttagcgaag agaaagggaa atacgtcggt ggagcagaaa gtattaagca
    23221 acaggtcgat gcttcgcgtt cgatggtgat agggcacacg ggtgacaaaa ttttcgacag
    23281 tattaccagc aacgcagttg cggagccaga cggtagcgct tctgaaacca atctttttgc
    23341 catgctggat agcgccatcg cagccctgaa aacgccggtc gcggatagcg aagcggataa
    23401 agaaatcgcc gctgcggcat tggataaaac caaccgtgga ctgaaaaact cgctgaataa
    23461 tgtccttacc gttcgcgcgg aattaggcac gcagctgaac gaactggagt cgctggattc
    23521 cttaggtagc gatcgcgctt tagggcaaac gcagcagatg agcgatctgg ttgatgtgga
    23581 ctggaatgca actatttcat cttacatcat gcagcaaacg gcattgcagg catcgtataa
    23641 agcatttacc gatatgcagg gattgtcgct cttccagctc aacaaataag ctcgctttaa
    23701 aacatatcat gaaactgggt atgttttgtc tgcccgctca gggatcgccg gggcgggcat
    23761 ttttttgcct atttagcatt gttggttagc aaggatgcca tttgatgaat tctaatatgt
    23821 tgattcaaac atgaaataaa aaagccctgg cagttaccag ggcttgatta ctttgagcta
    23881 attattactc aacaggttgc ggacgcgcag gagcggcaga ggcatgatgc gttgccgtat
    23941 gaccacctgc ggcaccttta ccttcgaagg caaaagtagg gcgctgccag tcactgtgat
    24001 gcggtgcctc cggaacatat tccggtgccg gagcgcgcgt cattggcgcg gtagcgtggt
    24061 tatgctcaac ggtgatctct ggttcaaccg cggctaccgt ttcaacttct gctgcgactt
    24121 cagcgacgac tggcgcggca ggttgagcaa caacttcagg ttctgcaacc acaacctcag
    24181 cagtttcggc aacttcttca atatctgccg tctcttcctg cggttcaacc accggttctg
    24241 cgtgttctgc aacttcctgg gctacggcaa catcagactc ggtaatcacc tgcggctgtt
    24301 cagttaccgc ggcggcaatc acttcaggat gcgtcgtttc aaccacttcc ggttgcggtt
    24361 cggcaaccgg tacgggggct tctacgactt cagccatttc ttcaacaact ggcgcgctaa
    24421 caaccggttc gacagcggcg gcgacaggga cctcagtcac catcggctgc acttgtactt
    24481 cttcctgttc gcgctgctct tcaacttgta catcttgcgg acgtacaatt ggatagcgga
    24541 tccagacttt gccagaggcc agttccggag acgcgcacgc tacggtcaac ggcattggcg
    24601 actgggttgg ataacgctcg tcacgatagc gacgacgacg ctgaccactc acgcgcaggt
    24661 gacgaggcga gcggcgagaa cgacgcggca tgccaccgtt gtcacggtta tcagcattgt
    24721 tctcttcttg ctgttctggt gcagtttgcg ctacgactgg cagcgggact ttcaccagtt
    24781 ctgtgcgtgg agctggagct tcctgaacaa ttggttcggc agcggcagtt tcttcaacca
    24841 ccggtgcgac taccgcttct tcggctacgc tttgctcgta acgcactttc tgattgagct
    24901 gacgctgttt acgacgcggc tgaaccggac gtacacgttc ttcctgttcg gtttcctgaa
    24961 cagattgctc ttcaacattc agcgccttcg cttcttgttg cgcctgacgt ttatcatcgt
    25021 tacggcggcg gctacgttca cgacgcggcg cttgctgctc gtcggtggta cgtgcttttt
    25081 ccgttacctc agcttgctga cggctctcac gcgtctcggc agtctgctgc tgtgcctggc
    25141 gacgattacg acggttttct tcgcgattat cgctgccttc agtacgttca ctacgggtgt
    25201 cgcggcgctc ataacggtca cggcggttgt tctgacgagg cttgcgacga tcctgttgac
    25261 gttccggttt cgcttctgct ttcggtgctg gttgctcggt cggtttggtt tcttcactac
    25321 cgctgaacag cgctttcagt gcgccgaaga agcggctcaa cagcccaggt tgtgcaggag
    25381 ctgctggtgt tgccggtgca gctttcggtg ctggagctac aacaggcgcg gcaggttcag
    25441 ctggcgttgg cgctggcggc acatccggca tggcaaaggt tgccagcgca ggttgttccg
    25501 gacgcttacg ttcagcgaac tcttcttcag acggcagcgc catcgcttct tcatgcagct
    25561 tcggcagcat gtagcttaag gttggggttt cttccccttt acgcacgcgc agcacgtggt
    25621 agtgcggggt ttccatctga tcgtttggca caatcacgca gcgcacaccg tcctgacgag
    25681 tttcaatggc atttaccgca gaacgttttt cattcagcag gtaagaagcg attggtacag
    25741 gaacaatggc gtgaacttcc tgggtgttct ctttcagcgc ttcttcttcg atcagacgca
    25801 gaatagagag cgacagcgat tcgttgtcac gcacggtgcc agtaccagaa caacgcggac
    25861 aaacgtgatg actggattca cccagtgatg ggctcaggcg ctgacgggac atttccagca
    25921 ggccaaagcg agaaatatgg ctgatttgaa tacgcgcacg gtcctgacgc accgcttcac
    25981 gcagacggtt ttctaccgca cgctggtggc gtacaggcgt catgtcgatg aagtcgataa
    26041 caatcaggcc gccgaggtca cgcaggcgca gctgacgagc aatctcatcg gcagcttcga
    26101 ggttggtgtt aaacgcggtt tcttcgatat cgccgccgcg ggtcgcgcgt gcggagttga
    26161 tgtcgatggc agttaacgct tcggtactgt cgataacaat cgaaccaccg gacggcagac
    26221 ggacttcacg ctggaaggcg gactcgatct gcgactcgat ctggtagtgg ctgaacagcg
    26281 ggatctcgcc ggtgtacagt ttgattttgc tgctgaaatc cgggcgacct aatgcagcga
    26341 tatgctgacg tgccagttcg agcactttcg ggttatcgat aaggatttcg ccgatgtcct
    26401 ggcgtaagta atcgcggaaa gcgcgaacga ttacgttgct ctcctgatga atcaggaacg
    26461 gggccgggcg gctttcagcg gcttttttga tggcttccca gtgtttcaga cggaagctta
    26521 aatcccattg cagcgcctca gcagatttgc cgacgccagc ggtgcgcacg ataagcccca
    26581 tgccttccgg cagttcaagg cttgccagtg cttcttttaa ttcggtacgg tcgtcgcctt
    26641 cgatacggcg agaaatgcca cccgcgcgcg ggttgttcgg catcagaacc agatagctac
    26701 ccgccagact gataaaggtg gttaatgccg cgcctttgtt gccgcgctct tctttatcga
    26761 tctgaacaat gacttcctga ccttcacgca acacatcttt aatgttggga cgaccatgag
    26821 cactgtagtt agcagggaaa tattcgcggg caatttcttt tagtgggagg aaaccgtgac
    26881 gttcagcgcc gtaatcaaca aaagcagctt ccagactcgg ttcaatgcgg gtgattttac
    26941 ctttgtagat gtttgccttt ttctgctcgt gccctggact ttcgatatcc aggtcataca
    27001 gacgctgccc atctacaagg gcaacgcgca actcttcctg ctgagttgcg ttgattaaca
    27061 ttcttttcat cgtaacttac tcattattct tacattgacg actaagctgc gggcaaagta
    27121 acgcctttcc gggtgtgaac cgatggcctc gtgtctagtc gcgtcgccaa cctcacggtt
    27181 atcgtcagct caaagaggcg cagagtgtcg gttgcccgtt tttcatgcgg aaaaacagcg
    27241 caattatcaa agaaacagac tgggtattac tctccagaaa tatttccatc tgccggtaag
    27301 gactgcaacc cgcagcccgc taactgcctg aaagatcaat acgtcttacg ccattgctgc
    27361 gtggatgatc ggtcgggcaa aatgggttat tccgtaaaat ttcttgtttt aacaaggatg
    27421 gacacggaaa cggcttcatt attccactgc aagccttgtt atagcaagat gacttttacc
    27481 atttatcacc cgcttactca cagtt
//
LOCUS       DAJTNA010000037        27256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTNA010000037 DAJTNA010000000
VERSION     DAJTNA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27256
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.037"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..324
                     /locus_tag="OO161_001331"
     CDS             <1..324
                     /locus_tag="OO161_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR5462466.1"
                     /translation="FAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDLFLFSDKIFS
                     IAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGGGGSSGGGG
                     ASGRW"
     gene            complement(384..1682)
                     /gene="eno"
                     /locus_tag="OO161_001332"
     CDS             complement(384..1682)
                     /gene="eno"
                     /locus_tag="OO161_001332"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5462467.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1770..3407)
                     /gene="pyrG"
                     /locus_tag="OO161_001333"
     CDS             complement(1770..3407)
                     /gene="pyrG"
                     /locus_tag="OO161_001333"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5462468.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3630..4421)
                     /gene="mazG"
                     /locus_tag="OO161_001334"
     CDS             complement(3630..4421)
                     /gene="mazG"
                     /locus_tag="OO161_001334"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5462469.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4492..4827)
                     /gene="mazF"
                     /locus_tag="OO161_001335"
     CDS             complement(4492..4827)
                     /gene="mazF"
                     /locus_tag="OO161_001335"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR5462470.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4827..5075)
                     /gene="mazE"
                     /locus_tag="OO161_001336"
     CDS             complement(4827..5075)
                     /gene="mazE"
                     /locus_tag="OO161_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR5462471.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5153..7387)
                     /locus_tag="OO161_001337"
     CDS             complement(5153..7387)
                     /locus_tag="OO161_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5462472.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7435..8736)
                     /gene="rlmD"
                     /locus_tag="OO161_001338"
     CDS             complement(7435..8736)
                     /gene="rlmD"
                     /locus_tag="OO161_001338"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5462473.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8793..11549
                     /gene="barA"
                     /locus_tag="OO161_001339"
     CDS             8793..11549
                     /gene="barA"
                     /locus_tag="OO161_001339"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5462474.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11781..13121)
                     /gene="gudD"
                     /locus_tag="OO161_001340"
     CDS             complement(11781..13121)
                     /gene="gudD"
                     /locus_tag="OO161_001340"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5462475.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13142..14482)
                     /locus_tag="OO161_001341"
     CDS             complement(13142..14482)
                     /locus_tag="OO161_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5462476.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTQVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAVPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14484..15854)
                     /gene="gudP"
                     /locus_tag="OO161_001342"
     CDS             complement(14484..15854)
                     /gene="gudP"
                     /locus_tag="OO161_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5462477.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGSLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(16271..16720)
                     /locus_tag="OO161_001343"
     CDS             complement(16271..16720)
                     /locus_tag="OO161_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5462478.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16738..17520)
                     /gene="truC"
                     /locus_tag="OO161_001344"
     CDS             complement(16738..17520)
                     /gene="truC"
                     /locus_tag="OO161_001344"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5462479.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17520..17849)
                     /locus_tag="OO161_001345"
     CDS             complement(17520..17849)
                     /locus_tag="OO161_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5462480.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(18471..19016)
                     /gene="syd"
                     /locus_tag="OO161_001346"
     CDS             complement(18471..19016)
                     /gene="syd"
                     /locus_tag="OO161_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5462481.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGYIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19084..19932
                     /gene="queF"
                     /locus_tag="OO161_001347"
     CDS             19084..19932
                     /gene="queF"
                     /locus_tag="OO161_001347"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5462482.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20044..21408
                     /gene="ppnN"
                     /locus_tag="OO161_001348"
                     /gene_synonym="ygdH"
     CDS             20044..21408
                     /gene="ppnN"
                     /locus_tag="OO161_001348"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5462483.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21965..23254
                     /gene="sdaC"
                     /locus_tag="OO161_001349"
     CDS             21965..23254
                     /gene="sdaC"
                     /locus_tag="OO161_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5462484.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRVILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWIAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23312..24679
                     /gene="sdaB"
                     /locus_tag="OO161_001350"
     CDS             23312..24679
                     /gene="sdaB"
                     /locus_tag="OO161_001350"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5462485.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVYLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24791..25546
                     /gene="xni"
                     /locus_tag="OO161_001351"
     CDS             24791..25546
                     /gene="xni"
                     /locus_tag="OO161_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5462486.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKSGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25651..26788)
                     /gene="fucO"
                     /locus_tag="OO161_001352"
                     /pseudo
     CDS             complement(25651..26788)
                     /gene="fucO"
                     /locus_tag="OO161_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26816..>27256)
                     /locus_tag="OO161_001353"
     CDS             complement(26816..>27256)
                     /locus_tag="OO161_001353"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5462487.1"
                     /translation="PSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVSILNRPIPAIHY
                     MIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIACEVNLEKALWLA
                     HEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6581 a         7291 c         6574 g         6810 t
ORIGIN      
        1 tttgcagtga tgttcttccc tttctggttt tttcatcaag gcagtaattt ttgtcgcgca
       61 tgtaaaagtg gagtctgtat ttcagcaatc tatcttttag atttattcct gttctccgat
      121 aaaatttttt ccattgctgt attttccttc ttttttactt tcaccatatt tatggtcttt
      181 acctgtttat gtgtacttca gaaaagagca tcaggtaaaa gttatcattc agacaacagc
      241 ggttccggtg gaggctcaag tggcggcgga tttagcggcg gtggcggttc ttctggaggt
      301 ggcggcgcat ccggccgctg gtaactcaac cataaaaaat gccatcccgg aggctggcat
      361 ttttaaatca gataaagtca gtcttatgcc tggcctttga tctctttacg accgttgtac
      421 ggtgcttttt cgcccagagc ttcttcgata cgaatcagct ggttgtattt agcaacacgg
      481 tcagaacggc tcatagaacc agttttgatc tggcctgcag cagtaccaac agccaggtca
      541 gcgatggtag cgtcttcagt ttcgccagaa cggtgagaga taactgcagt gtagccagca
      601 tctttcgcca tcttgattgc agccagagtt tcggtcagag aaccgatctg gttgaatttg
      661 atcaggatgg agttagcgat acctttttcg ataccttctt tcaggatctt ggtgttggtt
      721 acgaacaggt cgtcaccaac cagctggatt ttgtcgccca gaactttagt ctggtatgcg
      781 aaaccgtccc agtcagattc gtccagaccg tcttcgatag aaacgatcgg gtactgtttg
      841 gtcagttctt ccaggaagtg agtgaattct tcagaggtga acgctttgtt gccttcgcca
      901 gccagaacgt atttaccatc tttgtagaat tcagaagctg cgcagtccat cgccagagta
      961 atgtctttgc ccagttcata accagcagct ttaacagctt cagcgataac agccagagct
     1021 tcagcgttgg aacccaggtt cggcgcatag ccaccttcgt caccaacagc agtgttcatg
     1081 cctttcgctt tcagaacttt tgccaggtga tggaaaactt cagaacccat gcggatggct
     1141 tctttcacag ttttcgcgcc aaccggctga atcatgaatt cctggatatc aacgttgttg
     1201 tcagcgtgct caccaccgtt gatgatgttc atcatcggaa ccggcataga gtatttgccc
     1261 ggagtaccgt tcagttcagc gatgtgctcg tacagcggca tacctttagc agctgcagca
     1321 gctttggcgt tagccagaga tacagccagg attgcgttcg cgccgaattt ggatttgttt
     1381 tcggtgccgt ccaggtcgat catgatcttg tcaatgccag cctgatcttt agcatctttg
     1441 ccaatcagcg cctgagcgat cgggccgttt accgcagcaa cagctttggt tacgccttta
     1501 cccaggaaac gggatttgtc gccatcgcgc agttccagag cttcacggga accagtagaa
     1561 gcacctgacg gagcagctgc cataccgacg aaaccaccct ccagatgtac ttcggcttca
     1621 acagtcgggt taccacggga gtcgatgatt tcacgaccga tgatttttac gattttggac
     1681 attaggtttt cctcaagtca ctagttaaac tgaaactcca gacaaacaac gcgtacccag
     1741 ggtacgcgtt gccgttctaa cttttttact tacttcgcct gacgtttctg gaactcgctg
     1801 gcggctttca caaagcctgc aaacagcggg tgaccatcac gtggagtaga agtaaactcc
     1861 ggatggaact ggcaagccac gaaccacggg tgattcggaa cttcgatgat ctcgaccaac
     1921 tgatcatccc cggaacggcc cgcaacgcgc agacctgcat cttcaatctg tttcaacagc
     1981 atgttgttga cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc attgtacagc
     2041 tggcgaacca ggctatcgtc aaccaactgg cactgctgtg cgccgagacg catggtaccg
     2101 ccgagatcgc tcttctcgct acgaacttca acgttgccgt tttcatcgcg ccactcggta
     2161 atcagcgcca caaccgggta cttacagtct ggcacaaatt ccgtagagtt ggcgttctcc
     2221 atgttggcaa catggcgagc gtaatcaatt aacgccacct gcatacccag gcaaatgccc
     2281 agataaggaa tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca
     2341 cgatagccga aaccgccagg tacgaggatt gcgtccagac ctttaaggat ttcaacgccg
     2401 cgcgtttcaa catcttgtga atcgatcagt ttgatgttga cgctgacacg attcttcagc
     2461 ccaccgtgtt tcagtgcttc gatcactgat ttataagcat ccggcagttc aatgtacttg
     2521 ccgaccatac cgatggtgac ttcacttacc gggttcgctt cttcgaagat aacctgttcc
     2581 cattcggaca gattcgcttc cgggcagttt aagctgaatc gtttacaaat ataatcgtcc
     2641 agcccctgag atttcaacag gcccggaatt ttatagatgg aatcgacgtc tttcagagaa
     2701 ataaccgctt tttccggaac attacagaac aatgcaatct tcgcacgttc gttcgccgga
     2761 acagcgcgat ctgaacgaca aatcaggatg tcaggctgga taccgatgga gagcagctct
     2821 tttacagagt gctgagtcgg tttggttttg acttcaccag acgctgccat gtacggcacc
     2881 agcgtcaggt gcataaacag agtgtgctca cggccaattt caacagccat ctggcgaatc
     2941 gcttcgagga acggcaagga ttcgatatca cctactgtac cgccgatttc taccagtact
     3001 acgtcatgac cttcgccacc ttccagcacg cgctctttga ttgcgttagt gatgtgcgga
     3061 ataacctgca cggttgcgcc gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag
     3121 tagatacgac ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat gaaacgctcg
     3181 tagtgcccca ggtccaggtc ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt
     3241 tggattgggc tcatagtacc tggatcgacg ttgatgtacg gatccagttt catgatggtc
     3301 acattgaggc cacgggcttc aagaatggct gcgagggagg ctgcggcaat gcctttaccc
     3361 agagaggata cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga
     3421 agttaggttg aaaagacgat ggaataacca ggacgggaaa gcagtatacc cgaacatgac
     3481 ctgtgccaca aactttcatt atccctcctc ttcgccagcg cactattgaa atcaggagtg
     3541 agaaaatagc ccctttgggg taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa
     3601 aatgacaagc gcttgaccgc gtaattccct tagagatcaa tttcctgccg ttttacctgt
     3661 tgccagactt cttccattgt ttcgaggtca acacctgtca tttccagtcc acgcgcggca
     3721 acaatacgct ccacttcgcg aaaacgacgc tcgaattttt cgttcgcttt ttgcaatgcg
     3781 atttctgctt tcgtccctaa atggcgagcc agattaaccg tggcaaacag caggtccccc
     3841 atttcctcct ccagtttagc ctggtcgaca acagcctgcc gcgcctcgta catcacctcg
     3901 tcgatctctt cgtagacttt atcaaccacc gggccaagcg tcgtccagtc gaagccaacg
     3961 ttggcgcaac gtttctggat tttttgcgcc cgcattaaag ccggtaaact acgaggaata
     4021 tcgtccagcg ccgaatgctg cgctttctgc gcgcgctctt cggttttgat ttgctcccaa
     4081 cgggcaagca cttcactact gttttcggca gaactatcag caaaaacatg cggatggcga
     4141 cgctctaatt tatcgctaat agcagcgcaa atatcattaa agtcaaagcg cccttcttcc
     4201 tgagccattt gcgcgtaaaa caccacctgg aatagcagat cgcccagttc accgcgaaga
     4261 tcgtcaaaat cttcacgggc gatggcgtcc agcacttcgt aggtttcttc aagggtgtaa
     4321 ggcgcaatgg tggcaaatgt ctgctcttta tcccacgggc agccgttttc cggatcgcgc
     4381 aggcgctgca taatagtgag caaacgatcg atttgattca ttgaattgtc ctgaaaattg
     4441 cgggtctgtc aggtggaaac ctgtgaccag aatagaagtg agttagtaac actacccaat
     4501 cagtacgtta attttggctt taatgagttg taattcctct ggggcaactg ttcctttctt
     4561 cgttgctcct cttgcccgcc aggcgattct ttttacctga tcagctaacg ctacgccatc
     4621 acgttcctga ccggataaaa caacttcgaa cggatatcct tttgattgcg ttgtacaagg
     4681 aacacacaga cacatacctg ttttgttgtt gtacatgaaa ggactcagga caacagctgg
     4741 acgatgtcca gcttgctcgc taccttttgt cgggtcaaaa tcaacccaaa tcagatcgcc
     4801 catatcgggt acgtatcggc ttaccattac cagacttcct tatctttcgg ctctccccag
     4861 tcgatattct cgtggaggtt ttccggcgtg atgtcgttga ccagttcagc aagcgtaaat
     4921 acgggctctt tacgcactgg ctcaataatt aatttgccat ccaccaggtc aatcttcact
     4981 tcatcatcaa tattcagatt gagcgcctgc attaacgtag ccgggatccg caccgccggt
     5041 gaatttcccc aacgctttac gctactgtgg atcataaccc tttcctcaaa ccgctatcat
     5101 atgtagatac agtatatatc aatctacatt gtagatacga gcaaatttct gcctaactcc
     5161 cgtgcaaccg acgcgcgtcg ataacatccg gcacctggtt gagtttaccc agcacgcggc
     5221 ccagcacctg caggttgtaa atctcaatgg tcatgtcgat ggtcgccagt tgctgtttgg
     5281 tgtcgctacg gctggcaacg ccaagtacgt tcaccttctc gttggcgaga atggtcgtga
     5341 tatcacgtaa caacccacta cgatcattag ccaccacgcg gaccaccagc gaatatccgg
     5401 cggagtagct ctcaccccat accgcgtcaa caatgcgttc tggcgcatga gagcgcagtt
     5461 ccgccagttg ttcgcaatcg gcgcggtgta ctgaaatacc gcgcccctgg gtaatgaagc
     5521 cgacaatctc atctccagga atcggctggc agcagcgcgc gatgtggtgc atcaggttgc
     5581 caacaccttc gactaccacg cgaccgttat ctttactgcg gttttgcggc gtgtagcttt
     5641 tttgctgaag ttgcttcagc gcggcggcgt cctgctcttc ggcactcggc ttattaaatt
     5701 gcgattgcag gaagttcacc atctgattga gacggatatc cccgccacca atcgccgcca
     5761 gcaactcgtc gacatcatta aagttgtaac gcggcagcag atgtttttct gcttctttca
     5821 ggctgatccc cagatgttcc agctcgtcgt caaggatttg ccgcccagcc agaatgtttt
     5881 tgtcacggtc ctgtttacgg aaccaggcgt gaattttcga acgcccacgg ctggttgtga
     5941 cgtaaccgag gtttgggttt aaccagtcac ggctggggtt cggctgtttc tgggttataa
     6001 tttcaatctg gtcgcccatc tgcagctggt aggtgaacgg cacaatgcgc ccgccaattt
     6061 ttgccccgat gcagcggtgt ccgacatcac tgtggatgtg gtaagcgaag tccagcggcg
     6121 ttgatcccgc aggcaaatca acgacatcac ctttcggcgt aaagacatac acccggtcgt
     6181 caaagacctg gctgcgtact tcgtcgagca tttcgccgga atcagccatc tcttcctgcc
     6241 acgcaatcag tttacgcagc caggcaatcc ggtcttcatg tcccgaacgt gcgccgccag
     6301 ctgccgcgcc ctctttatat ttccagtgcg cagcaacacc caactctgca tcttcatgca
     6361 tctgtttggt gcggatttgg atctcaacgg ttttgccacc tggccccaga accacggtat
     6421 gaatagactg ataaccgttt ggtttcgggt tagcgacata atcgtcaaac tcatccggca
     6481 ggtggcgata gtgagtgtgc actatcccca gtgcggcata acaatcctgt aaacgctcgg
     6541 cgacaatacg taccgcacgc acatcaaaca gctcatcgaa ggcgaggttc tttttctgca
     6601 ttttgcgcca gatgctgtag atgtgtttcg gacggccata cacttcagct ttaacgcctt
     6661 cagctttcat ctcagcgcgc agatgaccga caaactcttc gatatagtgt tcgcggtcga
     6721 gacggcgttc atgcagcagt tttgcgatgc gtttgtattc ggtcgggtgc agataacgga
     6781 agcagtaatc ttccagctcc catttcagtt gcccaatccc aagacggttc gccaacggcg
     6841 cgtagatatt ggtgcactct tttgccgcca gtacgcgttc atcttccggc gcatctttta
     6901 cttcgcgcag atgagcaata cgctccgcca gtttgatgac cacgcagcga aaatcatcga
     6961 ccatcgccaa taacatccgg cgaacgttat cgacctgttc ggaggaaaca gaatcagtgt
     7021 gcgtcgcttt cagctggcgg atcgccgcca tatcacgcac gccgtgaata aggttaacga
     7081 cagacttacc gacgctctca cgcagcacat cttcgctgac tacgttggca tcggccagag
     7141 ggaacagcag cgccgcccgc agcgtgtcaa tgtccatact taatgtcgag aggatctcca
     7201 ccatctcaac accacgccac aataacagac tggcatccgg atgcccctgc gtctgctgca
     7261 gacaatacgc ccaggtttcg gctaagcact cacacgactt ctggctggta atacccagac
     7321 ttgcgatcca tttttccgga tcaaattcac cagccttatt gatatgtgca cttcttaccg
     7381 caaccattgt cctctccttt agggaccaga cctgccgaaa tcggcaaatc gtaactattt
     7441 aacgcgcgag aaaagtacca tcgattccag atgtcccgtg tgtgggaaca tatccagcat
     7501 cgccagtcgc gcaatggtat atcctgcttt taataacgct tcgctatccc gagccagcgt
     7561 tgcagggtta caggatacat aaactatacg aataggttcc agttttataa tttgctgcat
     7621 aacacctgcg gcacctgctc gcgccgggtc cagcaacact ttatcgaagc cgtttttcgc
     7681 ccacggctgc tttgtgacat cttcttcaag attttcgtga taaaacgtca cattctgtaa
     7741 gccattaaga cgcgcattct gctggccttt ttccaccagc gccggaacac cttctacacc
     7801 gaccacactg gcagcttgtg tcgccaatgg cagtgtaaag ttgcccatac cgcagaacag
     7861 atccagtacg cgatcttcag gttgcacatc cagccattcc aacgcactcg ctaccatttt
     7921 ttggttcaca cccgcattga cctgaataaa atcgcgcggg ctaaaagtta agcgcaaccc
     7981 gttagagtca taccagggca tctcaccaga gacggtttcg agtatctcac tatcgggggc
     8041 gagatacaga tccaggcctt cagaatgcga aaagcgttcc agtttttcgc gatctgccga
     8101 acttagcggt gcggtatggc gcaaaatcat cagcgtgccg ctggttgcct gtaccagttc
     8161 aacatgacca agatggcgca ttgcttgtaa gctgcccagg catgccctga ctttgggcag
     8221 caatgcttca agttggggca ctaaaatggg acattgttta acgtcgacaa tgtcactgga
     8281 gcccgctttg cgaaacccca tctgaagttg ctgtgttttc ggtaagtagt tcagacttaa
     8341 acgcgcgcga cggcgatagc cccagggaac atcggcgatc acttcagaga cttcgtgttt
     8401 cattaatcgg gcgagtgccg cacttttgct tcgctgctgt aaatccacgc tggcgtgttg
     8461 ttgctgacag ccgccgcata cgccaaaatg aggacagcgt ggcgtttcgc gttccgggct
     8521 atcgcttaac cggcgtacga ctttagcgcg ggcatactgt tttttatctt cagtaacagt
     8581 aacttccgcg ttttcctgcg gcaataatcc ggggataaat agcgttttgc cgttatgtcg
     8641 cgccacgccc tgaccaaaag agtcgaggtc gttgactgaa acggttatga tctgacgcgt
     8701 cgtcgtgcgt cgttttgcag agtagaattg cgccattggc gagactttct caatttaaca
     8761 gtgtgacctt aattgtccca taacggaact ccatgaccaa ctacagcctg cgcgcacgca
     8821 tgatgattct gatcctggca ccgaccgtcc ttattggttt attgctgagt atctttttcg
     8881 tcgtgcatcg ctataacgac ttgcagcgtc aactggaaga tgccggtgcc agcattattg
     8941 agccgcttgc agtttctact gaatatggca tgagcctgca aaatcgcgaa tctatcggtc
     9001 agttaataag cgtactgcat cgtcgccatt ccgatattgt tcgcgcgatt tcggtttatg
     9061 atgaaaataa ccgactcttt gtcacctcca attttcatct tgatccctca tcaatgcagc
     9121 tcggcagcaa cgtgccgttt cctcgccagc tcactgtcac tcgtgacggc gatattatga
     9181 tcctccgcac gccgattatt tctgagagtt actcccccga cgaatcgccc agtagcgatg
     9241 ccaaaaatag tcaaaatatg ttgggatata ttgcgcttga gctggatctt aaatcggttc
     9301 gcttgcagca atataaagag atctttattt ccagcgtgat gatgctgttt tgtatcggta
     9361 ttgcgcttat ttttggctgg cgcttaatgc gcgatgtaac cggtccgatt cgcaacatgg
     9421 tgaataccgt cgaccgcatc cgtcgcgggc aactcgacag ccgggtggaa ggatttatgc
     9481 tcggcgagct ggatatgctg aaaaacggta tcaactcgat ggcaatgtcg ctggctgctt
     9541 atcacgaaga gatgcagcac aatatcgacc aggcgacgtc cgatctgcgt gaaacgctgg
     9601 agcagatgga aattcagaac gttgagttgg atctggcgaa aaagcgcgcc caggaagcgg
     9661 cgcgtattaa atccgagttt ctggcaaata tgtcacacga gctgcgtaca ccactgaatg
     9721 gtgttattgg ctttacccgc ctgacgctga aaacagaatt aacaccaacg cagcgcgatc
     9781 acctgaatac gattgaacgt tcggcaaata atttgctggc aattattaat gatgttctcg
     9841 acttctcgaa actggaagca ggtaagctga ttctggaaag tattccattc ccactacgca
     9901 gcacgctgga tgaagtcgtt actctgctgg cacattcttc tcacgataaa gggttggaac
     9961 tgacgctcaa tattaaaagc gacgtgcctg ataacgtgat cggcgaccca ctgcgattac
    10021 agcaaatcat cactaacctg gtggggaatg caattaaatt caccgagaat ggcaacattg
    10081 atattctggt agaaaaacgt gcgctgagta ataccaaagt gcagattgaa gtgcagattc
    10141 gggataccgg cattggtatt cctgaacgcg atcaatcgcg cttattccag gccttccgac
    10201 aggctgatgc cagtatttcc cgccgtcatg gtggcaccgg gctggggctg gtgattacac
    10261 aaaaactggt taatgaaatg ggcggcgata tttcgttcca tagccagccg aatcgcggtt
    10321 caactttctg gttccacatt aatctcgatc tgaacccgaa cattattatc gaagggccat
    10381 ccacccagtg cctcgcaggt aagcgcctgg cctatgtcga accaaactcc gcagcagcgc
    10441 aatgcacgct ggatatttta agtgaaacgc cgctggaagt ggtttatagc ccaacgttct
    10501 ccgcgctgcc tcccgcgcat tacgacatga tgttgttagg catcgcggtg accttccgcg
    10561 agccgctaac aatgcaacat gagcgattag cgaaagcggt atcgatgacc gatttcctga
    10621 tgctggcact tccttgccat gcacaagtca atgctgaaaa actcaagcaa gatggtatcg
    10681 gcgcgtgtct gctgaaacca ttaacaccta cacgcctgtt gcctgccctg acggaatttt
    10741 gtcatcacaa acaaaacacg cttttgcctg taaccgatga aagtaagctg gcaatgacag
    10801 tcatggcggt tgatgacaac cccgctaacc tgaaacttat cggcgcattg ctggaagata
    10861 tggtgcaaca tgtggaactt tgcgatagcg ggcatcaggc ggttgaacgg gcgaaacaga
    10921 tgccgttcga tttgatctta atggatattc aaatgccgga catggatggc attcgggcct
    10981 gcgaactcat ccaccagctc ccccatcagc aacaaacgcc ggttatcgcg gtaacggcgc
    11041 atgcaatggc cgggcaaaaa gagaagctgc ttggcgcagg gatgagcgat tatctggcga
    11101 aaccgattga agaagagcga ttgcataatt tgttgttgcg ctacaagcct ggcagcggta
    11161 tttcctctcg cgtcgtgacg cccgaagtca acgaaattgt ggtgaacccg aatgcgaccc
    11221 tcgactggca actggcacta cgccaggcag caggaaaaac cgatttagcg cgcgatatgc
    11281 tgcaaatgtt actcgatttc ctgcctgaag ttcgcaacaa agttgaggaa cagctggttg
    11341 gagaaaaccc ggaaggcctg gttgatttga ttcataaact gcatggcagt tgcggctata
    11401 gcggtgtgcc gcgtatgaag aatctctgcc aactgatcga acaacagcta cgtagcggta
    11461 ctaaagaaga agatttggag ccggagctgc tggaactgtt ggacgagatg gataatgtcg
    11521 cgcgcgaagc cagcaaaatt ctcgggtaac ggacgtcttg tcgagtagca atcggctttt
    11581 tatgacgctg gtttcagatt ggtgacaaag tgcctgtgtt tatgccggat gcggcgttaa
    11641 cgccttatcc ggcctacaaa accaattaaa ttcaatgaat taaaaaatga tgtagacctg
    11701 atacatagca caacaggcta ttttgcgttt agcatcagtc tcaagccggc tccagataga
    11761 gccggttttg gttttctgtc ttaacgcacc atgcacgggc gcttgttatc gaacgtccag
    11821 ccaggaatca gatactgcat tcccatcgca tcgtcacgcg cgcaaagccc gtgtttctga
    11881 tacagctcat gggctttcat cacttgatcc atatcgattt ctacacccag ccccggtttt
    11941 tccggcacct gaaccagacc gcctttgatc tcaaacggtt ctttggtcaa gcgctgattg
    12001 ccttcctgcc aaatccagtg cgtatcaatc gcggtgattt tacccggtgc agcggcggca
    12061 acatgggtaa acatcgccag ggaaatatcg aagtggttgt ttgagtgtga accccaggtc
    12121 aggccaaatt catggcacat ttgcgccaca cgtaccgaac cttgcatcgt ccagaaatgc
    12181 ggatccgcca gcgggatatc aacggattgc agggagagcg tatggcccat ctgccgccag
    12241 tcggtggcga tcatattggt tgcggtcggc aggccagttg cgcgacggaa ctctgccatc
    12301 acttcacgcc cggagaaacc ttgctccgca ccacacggat cttctgcata agccagcgac
    12361 cctttcaggt atttaccaat tttaatcgct tcgttcagcg accaggcacc gttaggatcg
    12421 agcgtaatac gcgcctgcgg gaagcgttgc gccagtgcca caatagactc ggcctcttct
    12481 tccccggcca gtacaccgcc tttcagtttg aaatcgttga agccatattt ttcatacgcc
    12541 gcttccgcca ggcgcaccac cgcatccggc gtcatcgctt cttcatgacg caggcgatac
    12601 cagtcgcatg agtcatccgg ctggctttga tacggcagcg gtgtggcttt gcgatcaccg
    12661 acgaagaaca gataaccgag catttcgact tcgctacgct gttgaccatc gcccagcagg
    12721 gatgccacgt ttacccccag atgctgcccc agcagatcca gcattgccgc ttctatcccg
    12781 gtaactacat gaatagtggt acgcaaatca aatgtctgca aaccgcgccc accagcatca
    12841 cgatcggcaa aagtattacg caccagcgtc agaacgtttt tgtattcacc cagcgtttta
    12901 cctaccacca gcggaatcgc atcttccagc gttttacgga ttttctcgcc gccgggaatt
    12961 tcccctacgc cagtgtgacc agaattatct ttgataatca taatattacg cgtaaagaac
    13021 ggtgcgtgtg caccactcag attcatcagc atactgtcat gacccgccac cgggatgacc
    13081 tgcatttcag taacaacagg cgtcgtaaat tgagaactca tatttatgtc ctttttcaga
    13141 atcaatgacg gccgaaaacg ggacgtttac ggtcaaaggt ccagccgggg atcaggtact
    13201 gcatcggacc tgcgtcgtta cgcgcaccgc caggcagacg tttataggcc tcatgtgcct
    13261 tttgtacctg ttcccagtcc agttccacgc ccagaccggg cgcatcagga acggcaattt
    13321 ttccgttttt aatctccagc ggatttttgg tcaggcgaca atcgccctcc tgccaaatcc
    13381 agtgggtatc gatagcggta ggattacccg gtaccgccgc gcccacatgg gtaaacatcg
    13441 ccagagagat atcgaaatgg ttattagaat ggcagcccca ggtcagcccc cagtcgtcgc
    13501 aaagctgcgc cacacggact gcaccggaaa gcgtccagaa gtgcggatcg gcaagtggaa
    13561 tatctaccgc attgagcatc accgcatgac ccatttcgcg ccagttggtg gcgatcatgt
    13621 tagtcgcgac gggcaagccg gtcgcccgcc gaaactccgc cataacttca cgaccggaga
    13681 aaccctgttc tgcgccgcat ggatcttcgg cataggtaag aacatcattc agccctttgc
    13741 atagagaaat ggcttcatca agtagccagg caccgttggg atcaacggta atccgcgcat
    13801 cagggaagcg tttcttcaat gcacgaacag tgtcgatttc ttgctcgcca ggtaacaccc
    13861 cgcccttaag tttgaaatct ttaaagccat agcgatcctg tgaggcttcc gccagacgca
    13921 caacggcttc gctgttcatc gctttctgat ggcgcaactg ataccactca tggttgcccg
    13981 gcgtattttc cagataagga agatcggttt tggtccgatc accgatataa aacagataac
    14041 cgaggacggt aatagtctcg cgttgcttgc ctggccccaa cagttcgcag accggaacat
    14101 tcagcgcctt acctagcagg tcaagcaagg cggcttccag cgccgccacg gcattaacgc
    14161 gcaattcaaa agtccaggca cctttgccga aggtatcaaa atcggctgcc tgattacctt
    14221 tatgcacctg ctgaaccact ttattcaggc gcgcaacttc ctggcccaga accatcggaa
    14281 tagcatcgac ctgtgtttga taaatcacct ctccgcccgg cgcttcacca atgccggtat
    14341 gcccggcgtt atcggtgagt accacaatat tgcgggtgaa atatgcgtta tgtgccccac
    14401 caatattaag caacatgcta tcgtgcccgg cgaccggaat gactttcata tcagtaataa
    14461 caggactgga ttgtgtcgtc atcttattgt cccgcgacag gtttcagctc gatacgcttg
    14521 atatcgccca ccagcaccag gtagctcagt accgcgatta aggcatgaac accaacataa
    14581 atcagcgccc cattaaagga gccagtcgtg ccaacgatat aaccaattgc gattggcgtg
    14641 acgatgccag aaatgttacc gaacatgttg aacaggccac cggaaagacc gctgatctct
    14701 tttggcgcgg tatctgccat tactgcccaa cccagcgccc cgatgccctt accgaagaag
    14761 gccagcgcca taaagccgat gatcatccac tcaacgttga cgtagttgca gaacaccatc
    14821 accatcgaca gcaacatgcc cattacgatc ggtgttttac gcgcaatgtt cagcgatccc
    14881 gtgcggcgca tcagccagtc ggaaataatc ccacccagca caccgccgat aaaaccgcaa
    14941 accgccggaa cggaagccac aaagcccgct ttcagaatcg acatcccacg cgcctgcacc
    15001 agataaaccg gaaaccaggt aatgaaaaag taagtcaggg cgttgataca gtactgaccg
    15061 atataaacgc cgatcatcat ccgtgacccg agcagttgtt tgatctgccc ccacttcacg
    15121 ctgaacggca ctttaacttt ggtgttttgc tgatccatat tgatcagcgc gccacccgcg
    15181 gcgatgtact ccagctcttt ctgatttacc cccggatgtt gatttggctc gtggatgact
    15241 ttcaaccaga taaagctgat gacaatcccc agactgccca taaagaagaa gacgtgtgac
    15301 cagcccactt catgcgtcag ccagcccata atcggcgcga agatcaccgt tgcgaagtat
    15361 tgagcggagt taaaaatcga caccgccgtt cccctttcct gcgccggaaa ccaggccgca
    15421 acaatgcgac tgttgccggg gaaagatggc gcttcagcaa gcccgaccag gaagcgcaga
    15481 gtaaacaggg caacgataat gccgaatcca ctaaagatat cgacgaagcc ttgcagcaag
    15541 gtaaacatcg accagataaa gatcgaccag aagtagacgc gttttgaacc aaaacgatcc
    15601 agcaaccaac caccagggat ctgcccgata acataagccc atgagaaggc agagaacaca
    15661 tagcccattc ccacgggatc aaggccgata tctttggcca tttccgaacc ggcaatagaa
    15721 agcgtagcgc ggtcgccgta gttgaaggat gtgacgataa acaacatcac cactatccag
    15781 taacgagcat tcgtgcgttt ttccacactg ctcgcagcct gacttaaaga actcgttgtt
    15841 gcactcctga aaattcgcgt tagccacgct cactctggac tgcgacatag ccaggaaatc
    15901 agaggtgacg tagggtgttt tttgccgttt ttataggtcg ttcgccgaat acggcgcgtg
    15961 tttatatctg gcaatagcag tataaaaagc gcgccatagc ggctcaccgt gcaacaacac
    16021 aacattaatg cgttcaatga ggcccgattt tggcattagc cctggacggt ggaatccact
    16081 tcacggaaat gaaaacaaga acaagaaagg aagggttaaa acgaagaaat aaagaagagt
    16141 atgaaatgga tcgcttgact ccaggcaaac gccagtaaaa atccgcgcta tgaagcagtt
    16201 tttactggca tttgcctgaa aagattcgat tcagcaccgc taaaacgaca tttaccgctc
    16261 gctgaacata tcaggacaac agcgtgcccc actgttcgac ccacggattt gattccgttt
    16321 ccggttccgg gttctcactg gcgtcaatca acagcatttc gccaacccgc tgagcgctct
    16381 gttcctgcaa caaggcatcg aactgtttgc cgccattgca gaaatttaca taactgctgt
    16441 cgccgagcgc aatcacgcca taacgcagat tcggctggaa gcccagacta tctttgattc
    16501 cctgaaagag cggcacaatg ctatcaggaa ggtcgccctg cccggtcgtg gacgtaacca
    16561 ccagaacata cttatcctga taaggcagcc agtcgcttaa ttcaggatct tcaaataccg
    16621 ttgctttgtg gccctgtgcg gtcagaattg cttctgcttc ttccgccact aacagcgaat
    16681 ttccgtacat ggtgccgaca aaaataccaa tttccgccat accgttttct ccctggatta
    16741 ggaacttatc tctccatcct gacccgatgg cgcactgaac tcaacccttt cattttcagg
    16801 aagcagaccg cgccagccaa attgtgataa cgcctgcatc caggtgtcgt ccaggcccgc
    16861 gtgaatagtc agcggctcgc cagtaaaagg atgcgtcaat gacaactgac tggcatgtag
    16921 cattaaccgc tggaggccaa aatgctcagc accgctgcga ttctggcgta aatcgccatg
    16981 tttgctatcg ccaataatcg gatgacgcaa atgggcaaga tgtcggcgaa gctgatgttt
    17041 gcgtccggtt ttcggctcca gttccaccag gccgtagcgc gtggtcgggt aacgtccggt
    17101 cgctaccggc atttctacgg tcgccagacc gcgataatgc gtcactgctg gctgcgggcc
    17161 tttatcttcg cgggcaaatt tatcagcgat tttgtccagt tcttccacca gtggataatc
    17221 cagcaccgct tcttccatca accagccgcg cacaatcgca tggtaacgtt tctggatttg
    17281 gtgctgttca aactgttgtg ccagcagccg tccggcctcg ctggataatc ccatcaacaa
    17341 cacaccagaa gtgggtcggt ccagacgatg agcagtaaaa acatgctgtc ctatctggtc
    17401 acgcacggtt tgcatgacca ctactttctc gtcgcgatcc agccagctac ggtgaaccag
    17461 ccagccggag ggtttattta ccgcaaccag ccattcatcc tgatagagta tttccagcat
    17521 tagctcgcat catccgcaaa aagagcatcc agtttttcca actcagccag aataagcgcg
    17581 tgttgcggat ggtccgtcgc cagcgccatt tcataatagg gtgcaacagc aaaagcgccc
    17641 ggtaacggct ggttgttatt taacaaatcg tgcattcgcg ggatcagcac ccactgcaac
    17701 cactccagtg gttccatggt gtccataaag aacggttggg tactattaaa ttgatgcggc
    17761 tggggttcat cgtttcgcca gtgctgatgt tcacgcagta acgcttcaag cgcctgcaac
    17821 tggagacgaa cgcggtcatg agtggtcata aagcaacctc aataagaaaa actgccgcga
    17881 aggatagcag gaataaaaaa agggagcact gtattcacag cgctcccggt tcgtttcgca
    17941 gcattccagc tacttttgtt gctccctgct catccttgac aacttttcct ctggccttgc
    18001 ggccaatcgt tcatcctgaa ctattgcttc ctgctcacac caccccgatg tgatacttca
    18061 tcctgaagtg tccttggcct tcctgaccag ccgaatcatc ctgaccggtt ctcattctcc
    18121 atcctggagg tgtcctttaa cgcgtcctgc gtcatcctct tcgcttcatc cagaagcctt
    18181 tccctgaaac accatcctgg tgtgtcctgc agaagtgtca tcatcctgat gttcacttcg
    18241 ttgtctgact ccctgtcgac gaagatagaa tcgtcttttt cgcgaagtct tacaaggcgc
    18301 ttacagacaa tgccttaagg aaaatttcat atgaaagtta agaattaaca atcaacattt
    18361 tgattttaaa tgaaaataac gattcttatc tttttaagta ttcgcttatt tcctggcgat
    18421 ctcctacaga gtctgtaaga gatctctcac aaccagatgg ggtatttgga ttacagaaga
    18481 ggcttaagtt gattaaggaa ttccgcaaga tttgaggcta aatgcgtccg tttacgtgtt
    18541 cccagcgtct ctttgcacac ttcaccggag agattacaca ccgagatgac ctctaactct
    18601 tcttccagcg tcgcgataaa tagcgtgggc ggcagtttta gccttttctg agtcaccaga
    18661 tgaccaatca gattttcctg cacccgacgg aagtcgtctt cactccaggt ttgcagcaat
    18721 gtcagtttga tatagccaaa ctgcgcgtgc atatccccgg caaactgagt cgtataaaag
    18781 gtatgaattg tgggttgtat cacaatatca aaagcgcgtt caaccgcgtt tacattttgt
    18841 tccccggtaa acggctgggg ttgccagtat acggcatctt cggtagttga aataatgcac
    18901 ggtgaaggaa caccgtacag ttcctcactt aacggccagc ttttatgctc ttcatgccac
    18961 gcatcgcagt agcgtgcagt aaaatctttc agggcttgtg cggtcaaatc gtccactaat
    19021 ttctctcttc acttaaacca gatacacttg tcgtttagtt tatctggttt atgacggtga
    19081 aacatgtctt cttatgcaaa ccatcaggca cttgcgggcc tgactcttgg aaaatcaacc
    19141 gattaccggg atacctatga cgccagccta ctgcaaggcg ttccacgtag cctgaatcgc
    19201 gacccgctgg gtctgaaagc ggataacctg ccttttcacg gtacggatat ctggacgctg
    19261 tatgaacttt cctggctgaa tgcgaaaggt ttgccgcagg tcgctgtcgg tcatgttgaa
    19321 cttgattaca ccagcgtaaa tctgattgag tcgaagagtt ttaagctcta tctcaacagt
    19381 tttaaccaga cgcgttttaa taactgggat gaggtgcgcc agacgctgga gcgcgactta
    19441 agcacttgcg ctcagggtaa gattagcgtg gcgttatatc gtcttgatga actggaaggc
    19501 cagccgatag gtcattttaa tggcacttgc attgatgacc aggatatcac tatcgataac
    19561 tatgaattca ctactgacta tctggagaat gccacctgtg gtgaaaaagt agtggaagag
    19621 acgcttgtca gccacctgct gaaatcaaac tgcctgatca cccatcaacc agattggggt
    19681 tcgatccaaa ttcagtatcg tggacgccaa attgacagag aaaaactgct gcgttacctg
    19741 gtctcattcc gtcatcacaa cgagttccac gaacagtgcg tggaacgcat ctttaatgac
    19801 ctgttacgct tctgccagcc agaaaaattg agcgtttacg cacgttatac ccgtcgtggc
    19861 ggtctggaca ttaacccgtg gcgcagtaat agcgattttg tcccatcgac cacaagactg
    19921 gttcggcaat aaattttttc tcaattttgc gtgctggatt cacgcagaag gttgtgaaag
    19981 gtcatcaggc agggctattg taatcaaagg gaatgacgat attcttccca taaggagttt
    20041 ttcttgatta cacatattag cccgcttggc tccatggata tgttgtcgca gctggaagtg
    20101 gatatgctta aacgcaccgc cagcagcgac ctctatcaac tgtttcgcaa ctgttcactt
    20161 gccgtactga actccggtag tttgaccgat aacagcaaag aattgctgtc tcgttttgaa
    20221 aatttcgata ttaacgtctt gcgccgtgaa cgcggcgtaa agctggaact gattaatccc
    20281 ccggaagagg cttttgtcga tgggcgaatt attcgcgctt tgcaggccaa cttgttcgcg
    20341 gtcctgcgtg acattctctt cgtttacggg caaatccata acaccgttcg ttttcccaac
    20401 ctgaatctcg acaactccgt ccacatcact aacctggtct tttccatctt gcgtaacgct
    20461 cgcgcgctgc atgtgggtga agcgccaaat atggtggtct gctggggcgg tcactcaatt
    20521 aacgaaaacg agtatttgta tgcccgtcgc gtcggaaacc agctgggcct gcgtgagctg
    20581 aatatctgca ccggctgtgg tccgggagcg atggaagcgc cgatgaaagg tgctgcggtc
    20641 ggacacgcgc agcagcgtta caaagacagt cgttttattg gtatgacaga gccgtcgatt
    20701 atcgccgctg aaccgcctaa cccgctggtc aacgaattga tcatcatgcc agatatcgaa
    20761 aaacgtctgg aagcgtttgt ccgtatcgct cacggtatca ttatcttccc tggcggtgtg
    20821 ggtacggcag aagagttgct gtatttgctg ggaattttaa tgaacccggc caacaaagat
    20881 caggttttac cattgatcct caccggcccg aaagagagcg ccgactactt ccgcgtactg
    20941 gacgagtttg tcgtgcatac gctgggtgaa aacgcgcgcc gccattaccg catcatcatt
    21001 gatgacgccg ctgaagtcgc tcgtcagatg aaaaaatcga tgccgctggt gaaagaaaat
    21061 cgccgcgaca caggcgatgc ctacagcttt aactggtcaa tgcgcattgc gccagatttg
    21121 caaatgccgt ttgagccgtc tcacgagaat atggctaatc tgaagcttta cccggatcaa
    21181 cccgttgaag tgctagctgc cgacctgcgc cgtgcgttct ccggtattgt ggcgggtaac
    21241 gtaaaagaag tcggtattcg cgccattgaa gagtttggtc cttacaaaat caacggcgat
    21301 aaagagatta tgcgtcgtat ggacgacctg ctacagggtt ttgttgccca gcatcgtatg
    21361 aagttgccag gctcagccta catcccttgc tacgaaatct gcacgtaatc tcccctcccc
    21421 cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat
    21481 ttgttatatg aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgtc
    21541 atacaattta tgttgcaacg caaacgtttc cctattttca taaaccgtat tttatcgcta
    21601 tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca
    21661 tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat
    21721 tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc caagtagatt
    21781 attgtatttg agatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc
    21841 aaattattac ggcggtaaat gattaagcca tcgccgatag acagatttca tttttacggt
    21901 caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa
    21961 atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg
    22021 caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct
    22081 gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct
    22141 tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa
    22201 aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa
    22261 actgattacc ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc
    22321 aatcaccaat acagttgaaa gcttcatgtc tcaccagctg ggtatgacgc caccgccgcg
    22381 tgtgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct tcggtgagca
    22441 gatgatcgtt aaagcgatga gtattctggt gttcccgttt gttggcgtat tgatgctgct
    22501 ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac
    22561 tgcatctgca accggaaacg gtctgtggat gaccctgtgg ctggcaattc cggtaatggt
    22621 gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta
    22681 cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt
    22741 gctgaccgta atgttcttcg tcttcagctg tgtactgagc ctgactccgg cagacctggc
    22801 tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcacc
    22861 ggttatcgcg tggatagctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg
    22921 ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa
    22981 aggtaagtca atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac
    23041 gacctggatt gttgctaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg
    23101 tccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc
    23161 aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg gtctgattgc
    23221 aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc
    23281 gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat
    23341 cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac
    23401 cgacgatctg attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta
    23461 cggctcgctc tctctgaccg gtaaaggcca ccacactgat atcgccatta ttatgggcct
    23521 ggcgggtaac ctgccggata ccgtggatat cgattccatc cctggtttta ttcaggatgt
    23581 gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga
    23641 tcagtgcatg aacttccacg ccgacaacct ttctctgcat gaaaacggta tgcgcattac
    23701 cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg gcggtggctt
    23761 tatcgttgat gaagagcatt ttggccagca ggatagcgca ccggttgaag ttccttatcc
    23821 gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg
    23881 cctgatgatg aaaaacgagc tagcgctgca cagcaaagaa gagctggaac agcacctggc
    23941 gaacgtatgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg aaggcgtgtt
    24001 gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca
    24061 ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc
    24121 agtgaacgaa gagaacgctg ctggcggacg cgtggtgact gcgccgacta acggtgcgtg
    24181 cgggattatc ccggcagtgc tggcgtacta cgacaagttt atccgcgaag tgaacgctaa
    24241 ctcactggct cgttacctgc tggtagccag tgccattggt tctctttata agatgaacgc
    24301 gtcaatttct ggtgctgaag tcggctgcca gggtgaagtt ggtgtggcgt gctcaatggc
    24361 ggcggctggt ctggcagaac tgttaggtgc aagcccggcg caggtgtgca tcgcggcgga
    24421 aatcgccatg gagcacaacc tcggtctgac gtgtgacccg gtcgccggac aggtccaggt
    24481 gccatgcatc gagcgtaacg ccattgcggc agtaaaagcg gtgaacgccg cacgtatggc
    24541 gctacgccgt accagcgagc cgcgcgtcta cctcgataaa gttatcgaaa ccatgtacga
    24601 aacaggtaaa gatatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcaatgaa
    24661 gatcgttgcc tgcgattaat cgctctccag aggcctcgtt ttgcgaggcc tcttcccgat
    24721 ttctcatcca gccgtagcct gttccggcat cgaatgttac ccttatcgcc tgatctttaa
    24781 gggggttatc gtggctgttc atttgcttat tgtcgatgca ctgaatctta ttcgtcgcat
    24841 tcatgccgtt cagggatcgc cctgtgtcga aacctgccag catgcgctcg atcagctcat
    24901 tatgcacagc cagccaaccc acgcggtcgc cgtttttgat gatgaaaacc gcagtagcgg
    24961 ctggcgtcat cagcgtttac cagattacaa atcgggtcga ccgccaatgc cggaagagtt
    25021 gcacgacgag atgcctgcat tacgcgccgc ctttgagcaa cgcggcgtcc cgtgctggtc
    25081 aaccagcggc aacgaagccg atgacttagc cgccacgctg gcggtcaaag tgacacaggc
    25141 cgggcatcag gcaacgattg tttcgacaga taaaggctac tgtcagttac tttcaccgac
    25201 attacgtatt cgtgattact tccagaaacg ttggctggat gcgccattta tcgataaaga
    25261 atttggcgtt caaccgcagc agttgcccga ttactgggga cttgcgggga tcagcagttc
    25321 aaaggtaccg ggtgttgcgg gaatcggacc aaaaagcgcc acgcagctgc tggtcgagtt
    25381 tcagagtctg gaagggatat atgagaatct ggatgcggtt gccgaaaagt ggcgcaaaaa
    25441 attagaaacc cataaagaga tggcgtttct gtgccgcgat attgcccgct tacaaaccga
    25501 tttgcatatc gacggcaatt tacagcaatt gcggttggta cggtaacggc gagccggatg
    25561 caaacgatta tcaacacatt gaatttaatg cacttatata ccggataaga cgcggctagc
    25621 gtcgtatccg gcattatcac atcagcgcat ttaccaggcg gtatggtaaa gctctacaat
    25681 atcctcaagc gttgcttcac gcgggttgcc accggtacaa acatcatcca gtgccgcctg
    25741 cgccagtgcc ggaatgtctt ccttgcgtac accaacatca cgcaaatgtg gcggaatacc
    25801 gacatcacgg ttgagagcaa acaccgcttc aacagcggca ttacgcgcct cttccagcca
    25861 ctttcacgcc cataacgcgc gcgatatcgc ggtacttctc accggtaaag tcagcgttat
    25921 agcgcatgac atgcggtaac aggatggcgt tcgcaacacc gtgtggagtg ttgtaaaacg
    25981 cgcccagtgg atgcgccata ccatgcacca accctaaccc aacattcgag aagcccatac
    26041 ccgcaacata ctgcccgagc gccatttctt ctccggcatc cttatcacca gcaaccgatc
    26101 ctcgcagcgc cccagcaatg atttcaatcg ctttaatgtg cagtgcatcg gttagcgccc
    26161 acgcgccacg ggtaatatac ccctcaatag catgagtgag cgcatcgaca cccgtcgcag
    26221 ctttcagcgc tggaggcata ccatccatca tgtcagtgtc aataaacgcc acctgcggga
    26281 tatcatgcgg atcaacgcaa acaaacttgc gccgtttctc ttcgtcagtg atcacgtagt
    26341 taatggtcac ttctgccgca gtacctgctg tggtaggaat tgccagaatc ggtacactgg
    26401 gtttattggt cggggaaagc ccttccaggc tacgcacatc ggcaaactcc gggttgttgc
    26461 tgataatgcc aatcgcttta caagtatcct gtggagaacc accaccaata gcgatcaggt
    26521 aatccgcgcc gctattctgg aatacaccga gcccttcttt gacgacagta attgttgggt
    26581 tgggcactac gccgtcgtaa atcgcccatg ccagccctgc agcatccatc ttatcggtca
    26641 ctttcgccac cacgccgcat tgcaccagcg ttttatcggt gacgatcagc gccttctgat
    26701 aaccacggcg tttcacctca tcggttaaag ccccaacagc accccgacca aaccatgccg
    26761 tttcgttcag aatcattctg ttagccatca tccttctcct tgttgcttta cgaaattact
    26821 cttcaatccg taacccatag gttttgaatt tctccagcac tacggcaatc tcttcatcgc
    26881 tcagcactgg caccgggtcc gtaatcgcca gggtcgtcag gtaaagttgc gccagcactt
    26941 caacttcatg cgccagccat aacgcttttt ccagattcac ctcacaagcg ataagcccat
    27001 gatgttgtaa caaagttgcc ttacgatttt tgagagccag cgcaacatgt tcagaaagtt
    27061 cgcgtgttcc aaaggtcgca taaggcgcgc aaggaataga attaccacct gccgccgcaa
    27121 tcatgtagtg aatagcgggg atcggtcggt taagaatgga aactgctgtg caatgaacgg
    27181 catgattgtg aacaaccgcg ttggcatccg gtctgctttg ataggccgcc atatggaaac
    27241 gccattcgct tgaggg
//
LOCUS       DAJTNA010000038        26773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTNA010000038 DAJTNA010000000
VERSION     DAJTNA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26773
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.038"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..328)
                     /gene="rpnB"
                     /locus_tag="OO161_001354"
     CDS             complement(<1..328)
                     /gene="rpnB"
                     /locus_tag="OO161_001354"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5462488.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDA"
     gene            complement(525..1298)
                     /gene="hisP"
                     /locus_tag="OO161_001355"
     CDS             complement(525..1298)
                     /gene="hisP"
                     /locus_tag="OO161_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5462489.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1306..2022)
                     /locus_tag="OO161_001356"
     CDS             complement(1306..2022)
                     /locus_tag="OO161_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5462490.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(2019..2705)
                     /gene="hisQ"
                     /locus_tag="OO161_001357"
     CDS             complement(2019..2705)
                     /gene="hisQ"
                     /locus_tag="OO161_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5462491.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2795..3577)
                     /gene="hisJ"
                     /locus_tag="OO161_001358"
     CDS             complement(2795..3577)
                     /gene="hisJ"
                     /locus_tag="OO161_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5462492.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3798..4580)
                     /gene="argT"
                     /locus_tag="OO161_001359"
     CDS             complement(3798..4580)
                     /gene="argT"
                     /locus_tag="OO161_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5462493.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKCQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4846..5415)
                     /gene="ubiX"
                     /locus_tag="OO161_001360"
     CDS             complement(4846..5415)
                     /gene="ubiX"
                     /locus_tag="OO161_001360"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5462494.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(5510..7027)
                     /gene="purF"
                     /locus_tag="OO161_001361"
     CDS             complement(5510..7027)
                     /gene="purF"
                     /locus_tag="OO161_001361"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5462495.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(7064..7552)
                     /gene="cvpA"
                     /locus_tag="OO161_001362"
     CDS             complement(7064..7552)
                     /gene="cvpA"
                     /locus_tag="OO161_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5462496.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     GA"
     gene            complement(7812..8474)
                     /gene="dedD"
                     /locus_tag="OO161_001363"
     CDS             complement(7812..8474)
                     /gene="dedD"
                     /locus_tag="OO161_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5462497.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8464..9732)
                     /gene="folC"
                     /locus_tag="OO161_001364"
     CDS             complement(8464..9732)
                     /gene="folC"
                     /locus_tag="OO161_001364"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5462498.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALHASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9802..10716)
                     /gene="accD"
                     /locus_tag="OO161_001365"
     CDS             complement(9802..10716)
                     /gene="accD"
                     /locus_tag="OO161_001365"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5462499.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10872..11531)
                     /locus_tag="OO161_001366"
     CDS             complement(10872..11531)
                     /locus_tag="OO161_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5462500.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11614..12426)
                     /gene="truA"
                     /locus_tag="OO161_001367"
     CDS             complement(11614..12426)
                     /gene="truA"
                     /locus_tag="OO161_001367"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5462501.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTVPRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12426..13439)
                     /locus_tag="OO161_001368"
     CDS             complement(12426..13439)
                     /locus_tag="OO161_001368"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5462502.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13505..14641)
                     /gene="pdxB"
                     /locus_tag="OO161_001369"
     CDS             complement(13505..14641)
                     /gene="pdxB"
                     /locus_tag="OO161_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5462503.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14740..15735
                     /gene="flk"
                     /locus_tag="OO161_001370"
     CDS             14740..15735
                     /gene="flk"
                     /locus_tag="OO161_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5462504.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15732..16910)
                     /locus_tag="OO161_001371"
     CDS             complement(15732..16910)
                     /locus_tag="OO161_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5462505.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17194..18414)
                     /gene="fabB"
                     /locus_tag="OO161_001372"
     CDS             complement(17194..18414)
                     /gene="fabB"
                     /locus_tag="OO161_001372"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5462506.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDARRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18573..20579
                     /gene="mnmC"
                     /locus_tag="OO161_001373"
     CDS             18573..20579
                     /gene="mnmC"
                     /locus_tag="OO161_001373"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5462507.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20700..20978)
                     /locus_tag="OO161_001374"
     CDS             complement(20700..20978)
                     /locus_tag="OO161_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5462508.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(21012..21560)
                     /gene="epmC"
                     /locus_tag="OO161_001375"
     CDS             complement(21012..21560)
                     /gene="epmC"
                     /locus_tag="OO161_001375"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5462509.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21560..22369)
                     /locus_tag="OO161_001376"
     CDS             complement(21560..22369)
                     /locus_tag="OO161_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5462510.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22369..23193)
                     /gene="mepA"
                     /locus_tag="OO161_001377"
     CDS             complement(22369..23193)
                     /gene="mepA"
                     /locus_tag="OO161_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5462511.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(23197..24282)
                     /gene="aroC"
                     /locus_tag="OO161_001378"
     CDS             complement(23197..24282)
                     /gene="aroC"
                     /locus_tag="OO161_001378"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5462512.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24317..25249)
                     /gene="prmB"
                     /locus_tag="OO161_001379"
     CDS             complement(24317..25249)
                     /gene="prmB"
                     /locus_tag="OO161_001379"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5462513.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25415..25966
                     /gene="smrB"
                     /locus_tag="OO161_001380"
     CDS             25415..25966
                     /gene="smrB"
                     /locus_tag="OO161_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5462514.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRHEHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEVWLPPELP"
     gene            complement(26039..26752)
                     /locus_tag="OO161_001381"
     CDS             complement(26039..26752)
                     /locus_tag="OO161_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5462515.1"
                     /translation="MATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSR
                     VSMSACPGLEQYDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDD
                     DFPDYIGPTKVSSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMEN
                     GKLCNGNNFDERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDT
                     TSRQPIDSTCRFTYILNMY"
BASE COUNT         6595 a         7322 c         6761 g         6095 t
ORIGIN      
        1 ccgcatcaag atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta
       61 attcttccgg cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg
      121 ttttcacaga ccacaagagg tcggaataat attgccgcag gtcttcatca ataaaactgt
      181 ttggttccag tttaagcgtc gttaaatcac acaatttgcg cagcggcgcg ggaagatgaa
      241 tatcaataaa atcccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat
      301 gcggcgtgga agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac
      361 gcgacaataa ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa
      421 ttcagaagta tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat
      481 tccccatgaa aatactccgg cagcgttagc caccggagag gaaattattt cagcgatccc
      541 ttaaggaact gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgccc
      601 tcttcttcta ttttcccctg atggaggaaa atgacatgag tagaaacatg gcgagcaaag
      661 cccatttcgt gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataata
      721 cgcaacactt cgcctaccag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa
      781 acttccggtt ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa
      841 agatgcaccg gatatttccc ctgcgcacgt tcgtctatcc cgacttttgc cagatacttg
      901 accgcccgct cgcgcgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg
      961 acgttttcca gcaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg
     1021 cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg
     1081 cgcaccagat tgatcgtctg gccattgacc acgatcgacc cttcactcgg tttttcgagg
     1141 aagttaatgc agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt
     1201 acatctccgg cattcgcttg cagtgagacc cctttcagca cttcatgttc gccgtagcgt
     1261 ttgtgcaaat cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga
     1321 aggtttcaca tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata
     1381 agagatgatt aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc
     1441 ggcgttaata tcgcgggcta ttttcagcag atccggcaca gtggccgtaa acgccagcgc
     1501 agtagagtgc agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc
     1561 agaaggcaaa ataatgcaac gatacatttt aaaagtcgag aagccatagg ctctggcggc
     1621 ttcaatttcc ccatgcggaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc
     1681 gcaggtgtta agcgtcagcg ccagcacggt acagttcagg ccactgcgga agaaagcgtt
     1741 aaggaattcg gttcccttaa caatctcaag cgtgtacatg ccggaataga acaccagcaa
     1801 ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat
     1861 gtatttatta ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac
     1921 taccgacaaa ataagcagcc acagagtgat cgccacacca gtaaagcgat aaccgtcggt
     1981 ccacagcagc ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca
     2041 cccacggagt agcggcgctc aaggaacagc agcacaccat tggaaacagt ggtgaaaacc
     2101 aggtaaatca cgccacagac gatggcgaaa tagaacggtt cccaggtact tttgcctgcc
     2161 agttgggtgg ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg
     2221 aggatcacct gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac
     2281 atgatccgcc gaaacacttg cccacgagta aaaccgaacg ccgtcgccgc ctctatatgt
     2341 cctttcggca ctgccataaa agcaccacgg aacgtttcgg taaaataagc accgtagata
     2401 aaaccgagag tgataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc
     2461 gcctccgtca ccgtgtttag cgcaatctgc aaaccgaaga aaatcagcag catcaacact
     2521 aagtccggca cgccacgaat cagcgtggtg tacccttcga aaatcagccc cgaaagccga
     2581 ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag
     2641 ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac
     2701 aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac
     2761 ccgaagggag cgggctttct ttcaccgatg gcgattagcc accataaaca tcaaaatcga
     2821 agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt
     2881 tcagtgcttc gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa
     2941 acagtttttc atctttaaca gacgggcctc cgaatttgta atctttaccg acaggttgtt
     3001 tgaggaaacc ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag
     3061 tcaggtcaga ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg
     3121 cccaatgttc attaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc
     3181 ctttcagcga ctcgactgtc ggctgaatgt cagaattttt cgccaccacc aaacgagaat
     3241 cggcagcgta cagtttgtcg gtgaaggcta tttcttgctg acgtttttcc gtaatggaaa
     3301 gcgatgacat gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg
     3361 gattttcgac aaacgtacat tgcgtattga tgcgtttgca taattccttt gccagatcga
     3421 tatcgaagcc aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt
     3481 cggtaccgat gcggatgttt tgcggaatcg cagcaaatgc cgcagttgcg ctggagaagg
     3541 ccagaaccag agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt
     3601 ttatagggaa tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc
     3661 aaaatatccc gccccgttag acgtattatt tttcattaaa aatgcttaag tatgcatttc
     3721 aatgcaccac gatagtgcaa caaacctagc gtgcaccatg atgatcattc catcaggtac
     3781 agcttcccag cgacgtatca gtcaccgtag acattaaagt cgaaatactt tttcgccatc
     3841 ttgtcgtagg tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc agccgtcagt
     3901 tcagcatcat ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt
     3961 actgatgagc cagcaaaggc gaaatcttta ccagcaggtt gcttgaggaa tccttcgctg
     4021 gcagcaactt catcttgtaa cgcagcatcc agacgtcctg cagccagatc ggaatagacc
     4081 aaatcctggt tggcataggc caccacatcc acgcctttgc tacgccaggt ctcgttagcg
     4141 taagcttcct gggttgaccc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc
     4201 gttggctgaa tcggtgaacc tttggcagca atcaaacgag aatctgcggc gtacagcttg
     4261 tcggagaagg caatctcctg ctgacattta tcggtaatgg aaagcgacga aataatggcg
     4321 tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa agtcactggc aacccaggta
     4381 catttcacct gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa
     4441 tcacctttag catctttcga cgagaacggc gcataggtgg tatcggttcc gatacgtacc
     4501 gtctccggta gcgccgcata gctggaagcc gctgcggaga gaccgactaa caaagacaga
     4561 gcgagaatcg acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta
     4621 ttgtgttgtg tgtttgcagg ctcttttatg caggtcttat gccatcttga cgacaacagt
     4681 aacattcaac gttaaatatg ttaataagac gttgcattat tgtcctgaag ttgaagatag
     4741 caggtatagc ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat
     4801 ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca
     4861 gcgggcaaag agatcttcag gaagggttat tgcaaactgg tcaagaacac gattaaccgt
     4921 ctgatttatc acatcatcaa gggattgcgg gcgatgataa aacgccggaa caggaggcat
     4981 aatcaccgca ccgatttctg ccgcctgagt cattaaacgc agatggccta agtgcaatgg
     5041 tgtttcacgc acgcagagca ccaacgggcg acgctctttc agcaccacat ctgccgcacg
     5101 ggtcagtaag ccatcagtat agctatggac aatgccggaa agggttttga ttgaacaggg
     5161 taaaatcacc atccccagcg tctggaaaga accggaagag atgctggcgg aaatatcgcg
     5221 cgcatcgtgc gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag
     5281 ggataaggtc tggcgcgccg cctggctcat caccagatgc gtttcgatat ctgtgacatc
     5341 gcgcagaacc tgtaataagc gcacgccata aatcgcgccg ctggcaccgc tgatgcctac
     5401 aatgagtcgt ttcataaata gttgccctgt cagacttgca ggcagacttt gcagtatttc
     5461 ggcgggagtt gcaagtcagg gtgccagacc ggcaccctca gcgaaggcat catccttcgt
     5521 tatgcatttc gagattttcc acttcgttct gacgttgcac tgctttggcg tcatcattac
     5581 gtaacgtatc gaggaaatcg aggtagccct gatcaacatc tttggtgacg tagacgccgt
     5641 tgaacaccga gcattcaaac tgctggatat ccggattttc agcgcgaacg gcgtcgatca
     5701 gatcgttcag atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcaa
     5761 cttcgcgacc gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg
     5821 ggaagcgaat ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg
     5881 ccatctcgat aatctgctca gaagtggtgc cacggacgat ggagtcgtcg accagcagga
     5941 cgtttttatc gcggaactcg gcgcggttgg cgttcagttt acggcgcacg gacttacgac
     6001 gcagctgctg gcccggcatg atgaaggtgc ggccaacata gcggttttta acgaagccct
     6061 ggcggtacgg cttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttccg
     6121 ggatcgggat caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcaccca
     6181 gtttcgtgcc catattcaca cgcgcgctgt aaacggaaat tttgtcgata aacgagtccg
     6241 ggcgggcaaa gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt
     6301 gacgggtaaa caactgccct tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac
     6361 gcaggaaatc aaagcccagc gtatcgagcg ctacgctttc ggaagcgacc atatattctg
     6421 tacggttctc gtcaatatca cgttttccca gtaccagcgg acgaatcccg tttggatcgc
     6481 ggaaagcaac cataccgtgg ccgataatca tcgccacaca ggcatatgcg ccgcggatta
     6541 agcggtttgt ggcagcaatg gcagcgaaaa tattgtcggc ttccagcggg tagtggcgga
     6601 agttgtccag ctcgctagcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga
     6661 tgtggcggcg tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc
     6721 cgttgtgggc aagcgtaatg ccatacgggg agttaacgta aaacggctgc gcttcagagg
     6781 cgctggagct gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac
     6841 gctgcatatg gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga
     6901 agcagttatt ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca
     6961 ccgttaaggc atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac
     7021 cgcacatacg tctttttcct cgttaagcca catctcagag cacttacgct ccgggcaaga
     7081 aacttgacga gctttgcaga taatcaaaaa accatctgat gataaaactg aactgcggga
     7141 tcagctgtga tttgctccag tcttcgcttt tcgacacccc ggtaaaggag tcgagaaaga
     7201 agagaatggc ggcaacaatc aacacaccgc gcaacgcacc gaaacagacg cccagcaccc
     7261 gatcggtgcc tgacaacccc gttttctcca ccaactggcc tatcacgaag ttcacgattg
     7321 caccaacgat cagggtagcg ataaacagta ccgcgatggc aattccattt cgaaccagtt
     7381 cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga
     7441 aagcacaacc ccatgtcacc agcgataacg cttcacgaac aaagccgcgg atcaggctaa
     7501 ccagagagga aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc
     7561 cacgatttta cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa ggaaacgttt
     7621 gcgtagggat ttccttccca cgcatcaata aaaatgggcg ctgaaaaaat attcaacgcc
     7681 atcgactttt tatgcctttg cggcatcgtg caatgcgtgt cggatgcggc gtaaacgcct
     7741 tatccgacct acggttctaa cactgcccag gcctgataag acgcgccagc gtcgcatcag
     7801 gcaagactgt attaattcgg cgtatagccc attaccacgc cacttaagcc agaaagttgc
     7861 ttcaactcac ccagcgaacc tttcagctta tctttcgagg catccggccc aaccagaata
     7921 cgggtaattt taccctgcac tggcgtggat ggcgacgtat aaacccgata accggcaccg
     7981 cgcagcttac cgacaatctc attcacttta tcggcatttt tcagcgcacc cagttgcaca
     8041 acataggctt tacccgttgg cgctgctttt tcttccacga ccggcttcgg ttctggcttc
     8101 ggcgcaggtg gtgcttcaac cttcggttta ggcggctcca ccggtttcgg cttcggtggg
     8161 gcgaccggtg caggttccgg ttcaaactcg gtgttattgg ctgcaatagt ggccggatcg
     8221 agcgacggtg ctgcggcatc acctgcccgc acctcttccg ctgcgccttc cggcggctgc
     8281 gtcggcaacg cctgggtggc ggctggcatc atatcaggct catcgcgatc gcccgctttt
     8341 ggaaccagcg ggatagccgc aaactcatcc tgataatgtt ttttctgccc gtccagcagc
     8401 cctggaagta caatcacccc cagcgccacc agcacgatcg tgcccactaa ccgattctga
     8461 aacttacttg ccaccgcttc tcctcgcgtc aatcacttcc atgacatgtg cgaccgtgtg
     8521 gaaagaacca cacaccagca cggtgtcttc cgctttagcg tccgccattg cggcatccca
     8581 tgcctgcgca acgctatcaa atgatttgcc gttacccaaa tgctcaagca gttgttctgc
     8641 cgtggcaccg cgcggccctt ccagtggcgc acaataccag tcatcaacca cgcttttcaa
     8701 ccaggccaga gttccggcaa tatctttatc atgtagcata ccgataaccg ccagcacgcg
     8761 cccgtttttc ggtagcgctt tcatacgccc ggtgagatat tccgccgcat gtggattatg
     8821 cgcgacatca aaaataacgc gtggcgactc gctcacaatc tggaaacgtc ccggcaaaat
     8881 tgcgctggca atcccgtcgc gaatggcatt ttcactgact tccagcccgc tggcatgcag
     8941 tgccgccagc gctgttgcgg cattcggttg cgggacaagc ggcaacggca gattttccag
     9001 cgtgccgtga gcatcgctaa acgcccagtc atgatcggtg acggaatagt tccactcaac
     9061 gccccgacgt tgtaacagtg cacctttttc ctgcgccaca tcagcaatgg tagaaggcat
     9121 ttccggctca ccgacaattg ccggttttgc gctgcgaaag atgcctgctt tctcgcgacc
     9181 aatactttcg cgatctggcc ccagccagtc ggtatgatcc agcgcaatac tggttactac
     9241 cgcgacatcg gcatcgacaa tattggttgc gtccagacga ccgcccagcc ctacttccag
     9301 aatcaccacg tcaagttgtg cctgtttgaa cagccacaac gccgacagcg taccgtactc
     9361 gaaataggtc agggaaatat caccgcgtgc cgattcaatc tccgcaaaag aggcggtgtg
     9421 ggccgattcc ggcaattcct gaccctgcac acgtacgcgc tcggtataac gcaccagatg
     9481 cggcgaactg tagacgccca ctttgtaccc tgccgccatc agaatcgact ccagcgtacg
     9541 gcaggtggtg cctttgccat tcgtacccgc aacggtaaac acaaatggcg ctggtttcag
     9601 aacgccaaga cgagccgcga cctggctcac gcgctcaagg ccgagatcga tagttttact
     9661 gtgcaggttt tccagataag aaagccacga agccagaggc gacgcggctt gaggagtgcg
     9721 tttgataatc atggtatccg ctgattcgtt acggtgagaa tagcaaaagg gcagagccag
     9781 tggccctgcc cttatcagtt atcaggcctc aggttcctga tccggtaccg ggggtaccac
     9841 tacgccttca cgcggcgctt caggattcgg cgctggcaga ttcatcaact tcgccagaat
     9901 gctcgccagt ttcaggcgca tttccggacg acggacgatc atgtcgatcg cgcctttctc
     9961 gatcaggaat tcactgcgct ggaatcctag cggcagtttt tcgcgaacgg tctgttcgat
    10021 aacacgcgga ccggcaaagc caattaacgc tttcggttca gcgatgttga gatcgcccag
    10081 catggcgaaa cttgcagaaa caccgcccat cgtcgggtcg gtcagcacgg agatgtacgg
    10141 caagccgcgc tcctgcattt ttgccagtgc cgcagaggtt ttcgccatct gcatcagcga
    10201 catcagtgct tcctgcatac gtgcgccacc agaggcggag aagcagatca gcgggcagtt
    10261 atcttccagc gcctgctcaa cggcacgcac gaaacgtgca cccacaacag accccattga
    10321 accgcccata aaggcgaact cgaatgccgc agcgacaacc ggcattccat acagagtgcc
    10381 tttcatcacc accagcgcat ctttttcgcc ggtttctttc tgcgcagaag ccagacggtc
    10441 tttatacttc ttggagtcac gaaacttcag cacatctttc ggctcaagct cgctacccag
    10501 ctccacaagg cttccttcat ctaacaggct atgcaggcga ttacgcgctg tcatacgcat
    10561 gtgatggtca cacttcggac agacctcaag attacgttcc agctcagcgc ggtataaaac
    10621 ctgaccgcag ctatcacact tagtccacac cccttcagga atgctcgcct tgcgggtggg
    10681 agtaatgttg cttttaattc gttcaatcca gctcattagg gacctttctg tctgaacctg
    10741 gttcgatgcc agttttatct ttggggacgc ataatgccat ttttgccccc aacagaccat
    10801 gaatgttgca cattaaaaca taacagcccg aaactttgga taaaaaagtg gtcgaaccgc
    10861 ggagttactt tttattttgc ggcgcgtgcc gcagcgcgtt tgtgacgaat aaattcgaaa
    10921 accccaggca atattgaaac aaaaataatg ccaacaatca tcagcttaag gttgctctga
    10981 atgaagggga gcgtgccgaa gaaataaccc gcgtaggtaa aaatcagtac ccacaacagt
    11041 gcgccgatca cgttataagc ggcgaaatga cggtacgaca tgtggcccat tcccgcaaca
    11101 aacggcgcga acgttctgac gatcggtaca aaacgggcga gaataatcgt tttgccgcca
    11161 tgtctctcgt aaaactgatg agtcttatca agataactgc aacgaaagat tttcgaatcc
    11221 gggttactga acagtttgtc accaaataac cgtccaatgg tatagttcac tgcatcaccg
    11281 acaatagcgg caatcaacat aagcaccacc atcatatgga cattgagatc gttagtttcc
    11341 agcgacgcca atgcaccagc aacaaacagc agcgaatcac caggcaggaa tggcgttacc
    11401 accagccccg tttcgcagaa cagaatcaaa aacagaatgg cataaaccca gacgccgtac
    11461 tccgcgacca gctctgccag atgcacgtca atgtgcagga taaaatcgat gaggaaataa
    11521 atcaggtcca tatttgccta tgccttgtac tcgtcatctt tcaggctgta actacgttgg
    11581 ctacagcctg aaatgttccg agcattattc tcgttagtcc gccagaaata gcgggcccat
    11641 tggcggtttt gggagatcat accggtcagg gtaatccacc gcgaccagat acagcccttc
    11701 cgcttttgcc gttgctgccg ccagcgttct gtcctttgcc gccagcaact ctgctatcca
    11761 gctctccggc tggttgtggg caccgacttc catcaggctg ccgacaatat tcctgaccat
    11821 atgatgtaca aaggcattcg ctttgatatc taccaccaca taaggaccgt gacgcgtgac
    11881 attaatatgc ataacgttgc gccacggggt tcgggactgg cactgcaccg cacggaacga
    11941 ggtgaaatca ttctcgccca gcaagcattg cgcagcccga tgcatccgtt cagcgtccag
    12001 cggttcgtaa aaatgggtta cccctttact cagtaccgcc gggcgcagcc gatgattgta
    12061 gatgatgtag cgataacggc gagccgtggc gctaaatcgg gcatgaaaat catcaggtac
    12121 agctttaacc caacgcacgg cgatgtcacc aggtaaattc gcatttacgc ccaaggtcca
    12181 cgccgcgtct ttgcgcggca cggttgtttc gaaatgcacc acctgcccgg taccgtgtac
    12241 ccctgcgtca gtacgcccgg cgcagaagac ggtgatgggt tcgttcgcca cctgggagag
    12301 cgccttttcc agcttctcct gcacactgcg gacttcattc tgtcgttgcc agccgtaata
    12361 cttactgccg tcgtactcaa tgcccagcgc aattttataa actggcagtt gttgctggtc
    12421 ggacattagt acagatactc ctgcaccagt ttctcggcga tttttactgc catcagcgcg
    12481 ccgccaaagc gaacgttatc ggccaccgac cagaactgga cttgctccgg cataccgtag
    12541 tcattacgca cgcagccaac agaaagatgc ggcgtacccg aagcatcccc tacctgagtc
    12601 gggaattcgt tctcttcaga gagcacaata tcttcgccct gagcaaacgc atcacgcgct
    12661 tcttctgccg ccagcggacg cagtgcttca aagttgacca tctgggcatg accgtagaat
    12721 accggtgcct ggacgacgct tgccgaaatc atcagccctt cgtcctgcag gattttgcgt
    12781 acttcgtcaa cgatacgacg ttcttcacgc acgctacctt cgctatccgg caacaacggc
    12841 agcatattga acgccagttg acgaccgaag aaatcttctt cgtcaatcgg aatgccgttg
    12901 agcaatttcg cactctgccc cgctaacgca tcgaccgctt ttttgccctg ggcggaggct
    12961 gaaatcaggc tggtaacact gatacgcgac aaaccgccct gatcgattaa cggtttcagt
    13021 gccgccagca gctggctggt cagactgtct ggtacggcga tgacattccg gttgcggtaa
    13081 tcggtcagta caaacgggtt tacttccggc accaccagcg gtacgtcggg ttcgagagca
    13141 aacagtccgc tgctgtcgat caccaggcaa cctgagttgg tcgcttcttc aacccaggca
    13201 gcggtagctt ctttgcctgc gacaaaaaat gccagctgcg cctgcgtcca gtcgaattca
    13261 gcggcatcct gcacggtgat tgttttacca ccaaagcgca gttgttcgcc tgcgctttcg
    13321 ttacgtgcca gtgcataaat ttccccaacc gggaactgac gttcagccag cgtttcaagc
    13381 agggcttcgc ccacagcgcc agttgcgccc aggacggcaa tgttccagcc ttcagacatg
    13441 gtggtttact ccagaaatag caaagctccc tgccaatgtt acagcagaga gcatgaagaa
    13501 gagattaacg tgccggatga tgaacggcgt taaaacccag tttacacagc aatgatgccg
    13561 cactggcgtc atcacaaatt acatacagag atgaccattc acggcgctca agatagtttt
    13621 tgcgcagttt atcgaactca cccggtatcc cggcgacttt acgcagcggt gcgtcatcgc
    13681 ggcgcacatc atacaccaaa tgcaccagcc ttttcagcgt cggttgatcg agcgggccat
    13741 gcagcgtaat gcgaccaaac tctggcgcgg gcagtaatgt atccagcgca acgtgctgtt
    13801 catgcccaat aaacttgcta taggcttcaa acacttgcgt agtaccgcgt gctttacctt
    13861 ccagggtata acctgcgata tgcggcgtgc cgatatccac ttttgtcagc aactccacgt
    13921 taagttccgg ttcgccttcc cagacatcca gcactacgct taacttctgg ccttcattca
    13981 ggcaggtcag caacgcagta ttatcgacga ctgcgccacg gcaggcgtta atcagaatcg
    14041 ctcccggctt caggctacgg atcagttttt catccgccag atgcagcgtt ttgtacggcc
    14101 catctttgaa gagtggcgta tggaaagtca gaatatccgc atgctgaact aattcatcca
    14161 gcgagcggaa atcaccctca tccccacggt cagcgcgagg cggatcgcaa agcaatgttt
    14221 taatccctaa cgcttccagt cgcgcctgca atcgacgtcc aacgttacca acgcccacga
    14281 tccccaccgt acggtcgtgc agtgaaaatc catcgcgttc ggcaagcatc agtagtgagg
    14341 agaaaacata ttccaccacc gcaatcgcat tacagccagg tgcagcggaa aaaccaattc
    14401 ccgcctgctt caaccatgct tcatcgacat ggtcggtccc cgcagtggca gtgccaacaa
    14461 atttaatggg ttttcctgcc agcaaagatt cattcacttt cgtgaccgaa cgcaccatca
    14521 gcgcatccgc gtctgccagt tgagcgacgg ggattggacg cccgggaacc gcggtcacct
    14581 cacccaaacg gctaaataag tcgcgggcat aaggcatatt ttcatcaaca aggattttca
    14641 cgtttgtgtt acctgtatga gacgagagtt aaccggacaa gtgtgccata atctcgcggc
    14701 caggcatact tgcgaagatt tcaggtataa ggatacgtaa tgatacaacc tatttccggc
    14761 cctcctcctg ggcaaccacc aggtcaggga gataacctgc cgtctggcgc gggcaatcag
    14821 cctttatcca gtcagcaacg tacttcgctg gaaagcttaa tgacgaaagt gacctcactg
    14881 acgcaacagc aaagagcaga actgtgggcg ggtatcaggc acgatattgg tctgtcggga
    14941 gattcaccgc tgctttcgcg tcacttccct gccgctgagc ataatctggc gcaacgtctg
    15001 ctggccgcgc aaaaaagcca ttctgcccgc cagcttttag cgcaattagg ggagtattta
    15061 cgtctgggga ataatcgtca ggcggtcacg gattatatcc gtcataactt tggtcagacg
    15121 ccgctgaatc agctctcacc ggagcaatta aaaaccattc tcaccctgtt gcaggaaggg
    15181 aagatggtta ttccgcaacc gcagcagcgc gaggcgaccg accgtccttt attaccggcg
    15241 gagcacaatg cgctaaaaca gctggtgacc aaacttgcgg cggcaacggg ggaacccagc
    15301 aaacagatct ggcaatcgat gctggaactt tccggggtga aagatggcga gttaattcca
    15361 gcgaaactgt ttaaccatct ggtgacctgg ctacaggcgc gtcagacgct aagccagcaa
    15421 aatacgccga cgctggaatc actacagatg gcgctaaaac aacctttaga tgccagtgaa
    15481 ctggcggcgt tatcggcata tatccagcaa aaatatggcc tttctgcgca atcatcgctt
    15541 tcttctgccc aggccgagga tattcttaat cagctttatc aacggcgggt taaagggatt
    15601 gatccgcgtg atatgcaacc gctgcttaat ccttttccac cgatgatgga cacgttgcaa
    15661 aatatggcaa cgcgtcccgc gctgtggata ctgttagtcg cgattatcct gatgctggtc
    15721 tggctggttc gttaaccccg acgaaatgac agtatcgtga caataatacc cagcaccgca
    15781 gagatcgccc cggcaagaaa taccgaagag taaccaaacg tggtcgccag cattcccgcc
    15841 agcggcccgg agacgccgag ggcgatatcc tgaaacgcgg cgtaaccgcc cagtgcggtg
    15901 ccgcgaactt gtgaggggac gcgtttaacc acctccacgc ccagcgcagg aaagataagc
    15961 gaacatccgg ctccggttaa cgccgcgccc gctaatgcga cccatgcacc tggggcttgc
    16021 cagagcagca acaagcccac cgtttctaca agcagagaga caatcgccac tttcacgccg
    16081 ccaaaacggt ccggcatcca gccaaacatg acgcgcatca cgacaaatgc gccgccaaac
    16141 gcggtaagag taaagcccgc catcgcccat cctttgctgg caaagtagag cgaaacgaaa
    16201 gtcccgataa ccgcaaaacc aacgccttgt agtgccagcc ctaaccctgg tttccagata
    16261 agcccgacaa cgctccacag cgatggacgt tctcccgcca gggccggtac tttgcgcact
    16321 gtgccgttac aggcccacgc cagtaagggt aataccattg tggtgatcgc cagtgcggca
    16381 aaaccgtaat ggctatgaat caacaggcca agcggcgcac caacagcgag ggcaccgtaa
    16441 atcgccattc cgttccatga catcactttg ccagagtgtt ttgcccctac gatgcctaat
    16501 ccccaggtca gagcgcctgt cagtaactgg ctttcaccaa agccaagaat caaacgcccg
    16561 acgaccaaca gggcaaattt caacggtgcg gagacaggca aaatcgccgc cagcagcaac
    16621 gcgccgccag ccagaccaca agctaacatc ccctgaagcg ccgaacgttt tgcaccatat
    16681 tgatcggcca gtcgcccggc atagccacgc gtcagcaccg tagccagaaa ctgaatcccg
    16741 acggcaatcc cgaccatggt attgccatag cccagatcat gatgaacaaa cagcgggata
    16801 accggcaacg gcagccctac ggtcatgtag gtgagaaaaa ccgcaaaagc gatgcggaag
    16861 agcgaaaaat tggcagaaga tcgtgtttcg gtttggctta cagcagtcat gcattactcc
    16921 agaatgcagc gcaaggcgag gagtatcccc gtctcatctc tctggtttca gggttacagt
    16981 gcgttggcag gatttaacgc gtacgtcttt tcagaaggaa atcgacaaag cgggaagttt
    17041 gcctggaact ggcggcgatt gtcaatgatg tgaaaaaggg aaccatcagg ttcccttttg
    17101 cgttagtgcc ggaggccgca ttggcgcgta acgtcggatg cgacgctggc gcgtcttatc
    17161 cgacctactc cgactactcc gacctactgc gaattaatct ttcagcttgc gcattaccag
    17221 cgtggcgttg gtgccgccga agccgaagct gttagacata acggtggtca gttcgcgatc
    17281 ggtcgtttcg gtcacgatgt tcagacctgc cgcctgctcg tccagctctt caatgttgat
    17341 gctcggggcg ataaagccgt gttccagcat cagcagagag tagatagctt cctgtacgcc
    17401 agcagcgccc agagagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc
    17461 gccgaacact tcacggatag ctgccagttc tttcacgtcg ccaaccggag tcgaagtacc
    17521 atgagagttc aggtaatcga ttggggtatc aacgccgtgc atcgccatct tcatgcagcg
    17581 tactgcgcct tcgccagacg gagcaaccat gtctgcacca tcagaggttg cgccgtagcc
    17641 aacgatttca gcatagatgt gagcaccacg cgccagcgcg tgttccagct cttcaaccac
    17701 taccataccg ccgccgccag cgataacgaa accgtcacgg cgagcgtcgt aagtacggga
    17761 ggctttttcc ggggtgtcgt tgtatttagt agacagcgca cccatcgcgt cgaattcgca
    17821 ggccatttcc cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag
    17881 ttggatctgc tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat
    17941 ggagtagtta acgccatgaa ttttaaacgg cgtggcgagg caggcagaaa cgccggatgc
    18001 catcgctttg gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc
    18061 gtcagcgccg aatacctgga aacgcgggga accgccgcca gaacctgcaa tcaggccaac
    18121 gcgcgggtta ttctggtaag cttccggaga aaggcccgca tctgcgattg cctgctccat
    18181 agaaaggaat gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag
    18241 gccagtggta tccagtttta cgttgcccca gacgtggcta cgcatgccgg aatccttcag
    18301 ctcctgagag aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg
    18361 gttattaccg atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata
    18421 cctctgtaag tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg
    18481 aacaagtccg atcagccatt taatagagaa atttgcgcag ccttacacac atcgctaaga
    18541 tcgagccacc gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca
    18601 acctcgaatt taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt
    18661 ccaacgataa cgggctggaa gagacgcgtt atgtttttct gggaggcaac caattagagg
    18721 tacgctttcc tgagcatcca catcctctgt ttgtggtagc agagagcggc ttcggcaccg
    18781 gattaaactt cctgacgcta tggcaggcat ttgatcagtt tcgcgaagcg catccgcaag
    18841 cgcaattaca acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt
    18901 tagccttagc gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc
    18961 agtggccaat gcccttgccc ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc
    19021 tggatttatg gtttggcgat attaacgaac tgaccagcca actggacgat tcgctaaatc
    19081 aaaaagtaga tgcctggttt ctggacggct ttgcgccagc gaaaaacccg gatatgtgga
    19141 cgcaaaatct gtttaacgcc atggcaaggt tggcgcgtcc gggcggcacg ctggcgacat
    19201 ttacgtctgc cggttttgtc cgccgcggtt tgcaggacgc cggattcacg atgcaaaaac
    19261 gtaagggctt tgggcgcaaa cgggaaatgc tttgcggggt gatggaacag acattaccgc
    19321 tcccctgctc cgcgccgtgg tttaaccgca cgggcagcag caaacgggaa gcggcgatta
    19381 tcggcggtgg tattgcctgc gcgttgttgt cgctggcgct attacggcgc ggctggcagg
    19441 taacgcttta ttgcgcggat gaggcccccg cactgggtgc ttccggcaat cgccaggggg
    19501 cgctgtatcc gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt
    19561 ttacttttgc tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg
    19621 gcgtcacgca gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa
    19681 tggatttacc cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg
    19741 gcgttgcgac aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag
    19801 aactgacccg taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat
    19861 atcagttaca gaatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc
    19921 agcaagcaac acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgattcagcc
    19981 aaacgtcgac tctcccggtg tattcggttg ccgggcaggt cagccatatt ccgacaacgc
    20041 cggaattggc agagctgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc
    20101 cggcgaatca acatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt
    20161 acagtgagga cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccgcaggcac
    20221 agtgggcaaa agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca
    20281 cccgcgatca tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat
    20341 atgcgtcgtt ggcggagcag aaagatgagg cggtaagcgc gccggttttt gacgatctct
    20401 ttatgtttgc ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc
    20461 tggcggcgca gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa
    20521 acccgaatcg gttatgggtg cggaaattgt tgaagggtaa agcggttaag gcggggtaat
    20581 ctgctctcgc agtgtttgcc ggatgcggcg tgaacgcctt atccgaccta cgtgtgacgt
    20641 gtaggcctga taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact
    20701 tactgctgtg acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca
    20761 cgcggcgaca attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc
    20821 accgcctgag cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg
    20881 ccacgcaaat acccactggc aaacaactca tcatcactgg catggtcaat catgccgtcg
    20941 attaatgcca gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg
    21001 tggcaccggc ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc
    21061 gtgtgataat aattttgtag tgctttaatg aagcgtgccg gacgctcggg aatgccattt
    21121 gtcagataat ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg
    21181 aagtcgcctt ccagattgtc gcaactgacg ttaaacggat atcccgccgc cacacagaac
    21241 aaccagtcga gtgcctgagg cttcacttca acatcttcaa actggctttg cgtttgggca
    21301 tcacgcccat ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg
    21361 gcaatacacc agtgcgaaat ctcatgaatt gcgctggcat aaaagccgtg ggcaaagaca
    21421 attcggttat acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta
    21481 atcagacggg tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctca
    21541 tagtggtgtg tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc
    21601 tatcgtaaag taatttggca ctcatcaccg ccgagacgat aacgatcatc gggcggatca
    21661 gtttttgtcc tttgctcagc accagtcttg aacccatgcg cgcgccgagg aactgcccca
    21721 ccagcatgac aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac
    21781 cgccaatgtt tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga
    21841 atccgcacag cgtaacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc
    21901 cgtcataaaa accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc
    21961 gctggcgatc ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatta
    22021 ccagaatggg caaaatctgc cgtaagacat cagcctgaac gtattgcacc agcagtgcgc
    22081 cgctcattga gccgacaaag gtcatggcga tattgagttt ctgatcgctt aaactaacca
    22141 ctttgcggcg aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat
    22201 tggttgccag cgcgttagcg ggagacattc ccgcagccat caatgccgga atggtgagta
    22261 acccaccgcc accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa
    22321 ctcccagcaa cagcggggaa accataaaca ggctattaaa cgtttccatt agatcacgtg
    22381 ctcatccagt agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt
    22441 tgttgttcct ggttttggag gtgcaaacca gctttgcagt tctgccccgc aaccatcgcc
    22501 tggtggcggt aaaggttgat cttcacactc cagactatcg gcagggcaac gtaatcgtac
    22561 atgcatatgc gcgcgatgct ggaaccaagg tcgcactttg cgcaaccagt cgcgatcggt
    22621 gcccgcatca aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc
    22681 tttgtcctgg gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac
    22741 aacgtgttta ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga
    22801 ggtccagcga gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct
    22861 ggcatgaccg ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt
    22921 acccatgccc agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg
    22981 gtgaccgaaa tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg
    23041 caacgtgtca gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc
    23101 cggcacaggt tgggttattt tttgccacgg cgtcgctgcc aagctagcgc tactggcaag
    23161 cagagccagc agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt
    23221 cacatcggca ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat
    23281 cgcttcagcg atcggcactg cacggatccc gacacaggga tcgtgacggc ctttggtgat
    23341 catctcaact tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt
    23401 cggtttcagc gccatatgag caatgatttg ctgcccgctg ctgataccgc cgagaatgcc
    23461 gcccgcatgg ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg
    23521 cagcgccact acgtcaaagc cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat
    23581 cagcgcatgg gcgatgtcgg catccaggcg gtcaaatacc ggctcgccaa gtccggcggg
    23641 gacgccactg gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc
    23701 gcgcatcagt tcatctaacg cgtcgatttt gtccggatcc gggcagaaga acggattttg
    23761 ttcaaccagc gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaagcagcc
    23821 gcggatttca ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac
    23881 gcgcatggcg gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta
    23941 tttttgttcg taggtgtaat cagcatggcc tgggcggaaa acgtctttaa ttgcgctgta
    24001 atcctgagag cgctggtcag tgttttcgat caacaagcca atgctggtgc cagtagtaac
    24061 gccttcaaaa acaccggaga gaattttgac ctgatccggc tcgcggcgct gggtggtata
    24121 gcgcgatgtc ccagggcgac gacggtcgag gtcatgttgc aggtccgctt ccgtcagcag
    24181 aatgcctggc ggaacaccat cgacgatgca gccgagcgcc agcccatgcg attcgccgaa
    24241 ggtggttacg cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt
    24301 gtgtttgcgt gtttacttaa tctttataaa tcgcgaaata ttcacgggcc gccagcagct
    24361 gctctttggt gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg
    24421 gaacatccgg atattgttcc ataagatgta ccatgctgtt gccgacttca caaatcaaca
    24481 cgccatcatc agcaaggtaa tctgccgcgt ttccaagaat gcgacgcgtc agtttaaggc
    24541 cgtcggtgcc agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg
    24601 acatatcttc cgcgtcgaca tacggcgggt tagtgacaat cagatcgtac tgcactttcg
    24661 gcaagtcgcg gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt
    24721 cgatgttctg ttcagcaacc gccagcgcgt cgggagagat atccaccgcg tcgacttctg
    24781 catccgggaa ggcataagca caagcaatgg cgatgcagcc gctaccggta cacatatcta
    24841 aaatatgctg cggttgtttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa
    24901 tcggcgagcg cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg
    24961 ctttgttggt caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa
    25021 caatacgatg tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca
    25081 gcggcaggta gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc
    25141 cgtgaccgta ccagatattt gccgcgctga agcggctcac tgaccagcgc aacatgtcct
    25201 gaatggtttg cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca
    25261 gggcatgatc gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat
    25321 tcacgcgcgg tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt
    25381 aaactgtagg aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga
    25441 ggaggaccag gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac
    25501 gattgtccac cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca
    25561 ggagcaggct gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga
    25621 aggtccggtg aaatatgttc gcccggatgt cagccatttc gaggcgaaga aactgcgccg
    25681 cggcgattat tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa
    25741 gcaggaactg ggcgcgttga ttgccgcctg tcgccacgaa catgtgtttt gtgcctgcgt
    25801 gatgcatggg cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca
    25861 tccgcatgtg atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt
    25921 ggtgttgatt gaagtggaag tgtggttgcc gccggagttg ccctgaggag ttgagcgtgt
    25981 tgtgccagga gcgcggaatg gcaggggcga atgaccctgc caggtgagaa aaggtgcgct
    26041 aatacatatt cagtatgtaa gtgaatcggc aggtagaatc tatgggctgt cgagatgtcg
    26101 tgtccacgcg tagcttcata tcatgcagtt gcctggacgt aatcggttgc ggctcaggcg
    26161 tcaccgtcac tttggcatta tcacagccgg aagtcgagaa ggtcatctgc tgtgcgataa
    26221 aacggcagta gacaccacgc tcatcaaaat tattcccgtt acacagtttg ccattttcca
    26281 tcaggaaagt gggcagtgtt tgctcaataa caccacctgt tgactgcaac ctgacgactt
    26341 tgttatgtac aacataattt tcattccagg aaacatcata cggtgccagg ggcaccgccg
    26401 gacatgaaga actcaccttc gttgggccaa tatagtcagg aaaatcatca tcatacgttt
    26461 tagcgaaggg atctctactc ttaacaaacg acgacacaat ccacggacaa ccatgtaaat
    26521 tcccatcgta ataaacaaac atctttaatt cattacatgt ccatcccgtt ttggtctggc
    26581 tatcatactg ctctaatcca ggacaggctg acatggatac cctgctgtcc gaaattttcg
    26641 acatagatgg ttgagtagaa ctatcgccag tccacgaaac taaatccggc tgtgaattgg
    26701 gcatgaatgt agccttaaat atagcgtaat taactgaatc tggcgtcgct ataatcagat
    26761 gaaactcaaa ctc
//
LOCUS       DAJTNA010000039        26665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTNA010000039 DAJTNA010000000
VERSION     DAJTNA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26665
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.039"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            516..1415
                     /locus_tag="OO161_001382"
     CDS             516..1415
                     /locus_tag="OO161_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5462516.1"
                     /translation="MDKIHAIQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKEVLSNLNELDGLFQQDATSISGILRIDIPPGI
                     AKNLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            1464..2477
                     /gene="yhjD"
                     /locus_tag="OO161_001383"
     CDS             1464..2477
                     /gene="yhjD"
                     /locus_tag="OO161_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5462517.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            2888..4210
                     /locus_tag="OO161_001384"
     CDS             2888..4210
                     /locus_tag="OO161_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5462518.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4444..6504)
                     /locus_tag="OO161_001385"
     CDS             complement(4444..6504)
                     /locus_tag="OO161_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5462519.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLIKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEQLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(6574..7341)
                     /gene="pdeH"
                     /locus_tag="OO161_001386"
     CDS             complement(6574..7341)
                     /gene="pdeH"
                     /locus_tag="OO161_001386"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5462520.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            7573..8502
                     /gene="kdgK"
                     /locus_tag="OO161_001387"
     CDS             7573..8502
                     /gene="kdgK"
                     /locus_tag="OO161_001387"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5462521.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(8598..10094)
                     /locus_tag="OO161_001388"
     CDS             complement(8598..10094)
                     /locus_tag="OO161_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5462522.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(10315..11601)
                     /gene="dctA"
                     /locus_tag="OO161_001389"
     CDS             complement(10315..11601)
                     /gene="dctA"
                     /locus_tag="OO161_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5462523.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(11784..13772)
                     /gene="hmsP"
                     /locus_tag="OO161_001390"
     CDS             complement(11784..13772)
                     /gene="hmsP"
                     /locus_tag="OO161_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5462524.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIACELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLLLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(13854..17356)
                     /gene="bcsC"
                     /locus_tag="OO161_001391"
                     /pseudo
     CDS             complement(13854..17356)
                     /gene="bcsC"
                     /locus_tag="OO161_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            complement(17338..18444)
                     /gene="bcsZ"
                     /locus_tag="OO161_001392"
     CDS             complement(17338..18444)
                     /gene="bcsZ"
                     /locus_tag="OO161_001392"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5462525.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(18451..20790)
                     /gene="bcsB"
                     /locus_tag="OO161_001393"
     CDS             complement(18451..20790)
                     /gene="bcsB"
                     /locus_tag="OO161_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5462526.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVVAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYMPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(20801..23419)
                     /gene="bcsA"
                     /locus_tag="OO161_001394"
     CDS             complement(20801..23419)
                     /gene="bcsA"
                     /locus_tag="OO161_001394"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR5462527.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYFIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(23416..24168)
                     /gene="bcsQ"
                     /locus_tag="OO161_001395"
     CDS             complement(23416..24168)
                     /gene="bcsQ"
                     /locus_tag="OO161_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5462528.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYLWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(24180..24368)
                     /locus_tag="OO161_001396"
     CDS             complement(24180..24368)
                     /locus_tag="OO161_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5462529.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            24641..26125
                     /gene="bcsE"
                     /locus_tag="OO161_001397"
     CDS             24641..26125
                     /gene="bcsE"
                     /locus_tag="OO161_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5462530.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAEHDGRHWRRIPEPMRLLDDAVERSS"
     gene            26122..26313
                     /gene="bcsF"
                     /locus_tag="OO161_001398"
     CDS             26122..26313
                     /gene="bcsF"
                     /locus_tag="OO161_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5462531.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            26310..>26665
                     /gene="bcsG"
                     /locus_tag="OO161_001399"
     CDS             26310..>26665
                     /gene="bcsG"
                     /locus_tag="OO161_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5462532.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQM"
BASE COUNT         6212 a         7376 c         6768 g         6309 t
ORIGIN      
        1 gaatctccac ctgaatatgt taaaaagact taagtagtgg aagggtatta cccgcgagaa
       61 aaaataagaa ttcgccattt ggcggtggcc attctacaga gatgacgcgt agaaaatagt
      121 taccgatata aatagttaca gctaaacgcc tgaaattaca tgtcgagggc actatttaaa
      181 acaattttga ggatttcctt atattggtgg ttagtacgca tgcaattaaa aatgaaattc
      241 cgcgactaca agccaaaata acaaatggca atgagacaaa aataagcaca aatagccaat
      301 acgtcctcta ttcactttaa agggaatcac tgaaaaatac gctctgttta aggggattca
      361 cctttctcat aaagctattc cgcccttttc ctgctgagaa atcgccacat tcgtcatgac
      421 aacattgtga aacccggcat tagatgttag aaaaaacaat aaacaatgcg atatgcgcgt
      481 tactgtttcg ctgtgaataa caatcggtca aagaaatgga taaaattcac gcaatacagt
      541 tgttcatcaa agtcgcggag ctggaaagtt tttcccgcgc agcggatttc tttgctttgc
      601 caaagggaag tgtttcgcgc cagatacagg cactggaaca tcaacttggc acccagcttc
      661 tccagcgcac cacgcgacgg gtcaaactca cgccagaagg catgacctat tatcaacggg
      721 caaaagaagt gttgagtaat ctcaacgaac tggacggtct gtttcaacag gatgccacca
      781 gtatcagcgg tatattacgc atcgacatcc cgccaggaat cgcgaaaaac ctgttactgc
      841 cgcgcctgtc ggaatttctc tatctgcatc cgggaattga gctggaactg agtagccatg
      901 accgtccggt agatattctt catgatggtt ttgattgcgt gatacgcact ggcgcgttac
      961 cggaagatgg cgttatcgcc cgtcccctcg gcaaactgac catggtcaac tgtgccagtc
     1021 cgcactatct gacgcgcttt ggttatccgc aaagccccga tgatctgact tcacacgcaa
     1081 tagtgcgtta tacaccgcac ctgggtgtac atccgttagg ttttgaggtt gccagcgtta
     1141 atggcgtcca gtggtttaag tctggcggca tgttgacggt aaatagtagc gaaaactatc
     1201 tcgccgccgg tcttgccggt ctggggatta ttcagatccc gcgcattgcc gtgcgcgaag
     1261 ccctgcgtgc cgggcggctt attgaagtat tacctggcta ccgtgccgag ccgctctccc
     1321 tttcgctggt ttacccgcag cgtcgggagc tttcccggcg tgtaaacctg tttatgcagt
     1381 ggctggctgg cgtaatgaaa gagcacctgg actgaccgac tatacttttt aagaatgacc
     1441 acgacagaag gacaaaagag cggatgacgc aggaaaacga gatcaaacgt cccacccagg
     1501 atctggagca cgagccgatt aagcagttgg ataatagcga gaaaggcggc aaagttagcc
     1561 aggcgctgga aaccgtcacc accactgccg aaaaagtcca acgccaaccg gttattgcgc
     1621 acctgattcg cgcgacagaa cgctttaacg atcggctggg taaccagttt ggtgcggcta
     1681 tcacctattt ctcatttttg tcgatgatcc cgattttgat ggtgtcgttt gccgcagggg
     1741 gctttgtgct ggcctcccat ccgatgttgc tacaggatat cttcgacaaa attctgcaaa
     1801 acatcagcga tccgacacta gccgccacgt tgaaaaacac catcaacacc gccgttcagc
     1861 agcgtacgac tgtagggctt gtcggcctgg cggtggcact ttattccggc atcaactgga
     1921 tgggtaacct gcgtgaagcg attcgtgccc agtcgcgcga tgtctgggaa cgctcgccgc
     1981 aagatcagga gaagttctgg gtaaaatatc tgcgtgattt tatttcactg attggtttgt
     2041 tgattgcgct gattgtgacg ctttcgatca cctcggttgc cggttcggcg cagcaaatga
     2101 ttattagcgc cctgcacctg aacagcattg agtggctgaa accgacgtgg cgattgattg
     2161 gcctggcgat ttccatcttc gccaactatc tgcttttctt ctggatcttc tggcggttgc
     2221 cgcgccaccg tccgcgcaaa aaagccctga ttcgcggaac atttctggct gctattggtt
     2281 ttgaagtgat taaaatcgtg atgacctaca ccctgccatc gctgatgaaa tccccctctg
     2341 gcgcagcatt tggttccgtg ctggggctga tggcattttt ctacttcttc gcccgtttga
     2401 cgctattttg cgcggcgtgg attgccaccg ccgaatataa agacgacccg cgaatgccag
     2461 ggaaaacgca gccttaaaat aggccggatg cggcgtaaac gccttatccg gcatacgatt
     2521 ttattcggcc tggctccccg taggccggat aagatgcgcc agcatcgcat ccggctataa
     2581 tgcgcacata acctcttgaa actcgtcccc agagtctctt cagccatcta tttaggagca
     2641 aacaatttca ttctaactca taaccccagc atataaatcc aattggtaac ttttatttaa
     2701 cctgaaacca gttttatcca ctatttataa aattatgtga agcatttcat agaagaaaaa
     2761 tcactggcct aaacattatc ccctttttgc ctggtttttg accatttccg cgatttgtta
     2821 cacattgaaa tatcactttt gctgtgcgta atatggctat tcgttagcca aaaaataaga
     2881 aaagattatg caagcaacag ccacaacact cgaccacgag caagaataca cgccgatcaa
     2941 ctcgcgtaat aaagtccttg tcgcctctct cattggcaca gccattgagt tcttcgactt
     3001 ttacatttac gccactgcgg ccgttattgt gtttccgcat atcttcttcc cgcagggcga
     3061 tcctacggca gcaacgctac agtcgctcgc caccttcgcc atcgccttcg tcgcgcgccc
     3121 cattggctct gccgtttttg gtcattttgg cgatcgcgtt gggcgtaaag cgacgctggt
     3181 cgcctcgttg ctaacgatgg ggatttcgac cgtggtgatt ggtctgctgc caggctatgc
     3241 cacgattggt attttcgccc cgctgctgct ggcgctggct cgatttggtc agggtctggg
     3301 cttaggtggt gaatggggcg gcgcggcgct gctggcgact gaaaacgccc caccgcgcaa
     3361 acgtgcattg tacggctcct tcccgcagct gggcgcaccg attggcttct tctttgccaa
     3421 cggcactttc ttgctgcttt cctggctact gaccgacgag cagtttatga gctggggctg
     3481 gcgtgtgcca tttatcttct cggcggtgct ggtcattatc ggcctgtatg ttcgcgtgtc
     3541 gctgcatgag tcgccggtgt ttgagaaagt cgctaaagcg aaaaaacagg tgaagatccc
     3601 gctgggtacg ctgctgacca aacatgttcg cgtaaccgta ctgggtacgt tcattatgct
     3661 ggcaacctat acgctgtttt acatcatgac ggtctactct atgaccttta gtaccgctgc
     3721 cgcgccagtt gggcttggcc tgccgcgtaa cgaagtgttg tggatgttga tgatggcagt
     3781 tattggtttt ggcgtgatgg tgccagtcgc tggattactg gctgatgcct ttggtcgccg
     3841 taaaagcatg gtaatcatca ccacgctgat catcctgttc gcgctattcg cctttaaccc
     3901 tttgctcggt tctggcaacc cgattctggt ttttgccttc ctgctgctgg ggttaagtct
     3961 gatggggctg accttcggtc cgatgggcgc gctgttacca gagctgttcc cgacagaagt
     4021 gcgttacacc ggagcatcgt tctcttacaa cgtatcgtcg attctcgggg cttccgttgc
     4081 gccgtatatc gcagcctggt tgcagactaa ctacgggtta ggtgcggtgg ggttatatct
     4141 ggcggcgatg gctggcttga cgttaatcgc cctgctgctg acccatgaga cgcgacatca
     4201 gtcgttgtaa gtatcgtgtg ggcagccacc ctcaccccag ccctctccct gaaagggaga
     4261 gggagaaaag cggagcgaga tgttatatcg cgaacataac agccctcccc cagaaagtga
     4321 acggaaaagc ggtactcgtt gtttcaacac gcacaaaaca gtcccctcgc ccctttgggg
     4381 agaggattag ggtgagggga acacgctgct cccgaactca ccagcaaaca caatccaacc
     4441 tcatcacttc ttcatctgcg acaatatcgt ccgacactga ttagcctccc cttcggaagg
     4501 tgagatcagt gccaataacg ccgccgccgg tgttaccagc gttgccagtg ccgccgcaac
     4561 ggcaccacgg gcaatcagcg gtccggcttt caccccagcc tgcggatttt taaacgtccc
     4621 acgcacatac agcggcgaac gcagtgtgat aatccgaatc cctttactct ccggatcaat
     4681 agtcaaatcc agctgttccg aagcaaaact tgccgtgccg gtaacattaa tcaacgcgtt
     4741 ctcagtatcg aaagcaaaaa tctgcgggcg cgctacgccg ttggcaatat tcagattcgc
     4801 cgccgcgcag ttcacccgca cctcatcgtc accaaatatc gcaccgacaa tgtagttgcc
     4861 gacattcagc ccaacaatct ccatcaggtt gcggctcacc agcccgtcat tcatcaacag
     4921 tttcaggttg ccgttactgt tgcctaaaag cgccgccacc gagttaccgc taccgcgtag
     4981 ttccgcgtca ccgttcattt cccccagcgt cttctgcatc agttccacat cgggcatcag
     5041 ttctttcagt ttcagtcgac gagcctgaat atctgcccgc ccctgcatcg gctttttatc
     5101 gccttccaga tggatattcg ccgcaatgct gccgcccgcc atgccaaatt tcagcggttg
     5161 caggcgcagg tcagcatttt tgaggatgat atgagtagaa agatcgctaa tcggcaggct
     5221 actgccatgc tcgatacgtc ggcctttgaa gcgaacatcg gcatccataa cgtcccattt
     5281 atcggtttcg aagcggtcat aaggcagcac tttgcccgca ggctgaacgc ttttttcgcc
     5341 cttcttctgt tcagaccgtt tcgacttttc tgcccctttc cccgaatcaa cgccaatcaa
     5401 cggtcccagg tccgccagcc gcaattgccg cgactcgaca tcaccttcca gttttggtcg
     5461 tggctttccg gtggtgtaga ccagagaacc gtggatatcg ctatcgccaa ttcgcccatt
     5521 aaaaccgcga taatcaaaga ccgacgattt ttcagtgtcg attttcgcta ccagccgacc
     5581 atccgtttca aacggcgggg tatcgggcag cagaacgcct gtcagttcat agagatcacc
     5641 cagtgaatcg ccagaaaatt taagccgtaa atcgacaccg cccatcttca ttgggtcatt
     5701 cacgacgcca tcaaaagcaa cacgggtatt acctgaacgg aaatcagcct gtaccggaaa
     5761 cggcgtccct tcgccacgca gcgccagcat accgcctatt tttcccgtac ccgtgagctg
     5821 ttcaccgtta taacgtccct gcgccttcag gccaaaaacg taatcgccca ccttttcttt
     5881 atccgcttta cctttcgatc cagtaacttc gctgaacggc agcggcttgc ctaacggatc
     5941 aacaaaaatt tccagatccg ctttgcttac tttgtcatca atggcgatcc gcccttgatc
     6001 gaaaagaata ttatccagcc gaaacgacca tgccgacggc tttgcattcg cgtctttgtt
     6061 atcatcgttg gcgagattaa acgtccagtt attgttcttt tcggagaggc gaatcaggcg
     6121 cgcgtcgggc ttttcgagct tgatccacgg cagccagacc gttttgatca gcagcgccag
     6181 cggtgccagc gttgcctcta ctcgcggcaa atgcaccatc gtgacttcgg gaatatccgg
     6241 tggattgcca agaatgatgt cttctgcatg tacatgaggc cacggcaccc agctgcgcca
     6301 gccagtttct tgtttttgcc gctcccacac cacgcccaga tcgccacgga tagcgaacgg
     6361 acgattcaac tccgcagaga ctttctggtt gatggtcggt ttgagtcgat tccagtcaaa
     6421 agttgcaatc aaaatgatcg ccacgacaat caacaacaag aaagcccctg aaatcgcagc
     6481 ggttattttg cctgccttgc tcatcgttcg cttcctcata ttcttcctgt gccagtccta
     6541 aagatagtcc agccaggcgg aaaatgaggc agcttatagc gccagaaccg ccgtattcag
     6601 cgtttctatc ggtgccgggc gtgaaagaaa ccagccttgt gcggcgaatg cgggcgagtt
     6661 ctgtacatca cgccactctt ccggcgtttc tacgccctca acaatcaccc cgcgacaata
     6721 gcgattcatt agatgtaaaa gctgagaaaa gagtgtgcgt ccttccggcg actgacgcag
     6781 catcacaaac agttctcgcg cgattttgat gtagtcataa cgcacttcac ttagtgcaga
     6841 gaaatttgcc atcccggtac caaaatcacc cagccacagc gggccaaatt cacacatcga
     6901 ggcaaaggtt gaatctttcg gcagacggat atgctccacc agttcgaaac gcagccaggg
     6961 aagacgctca atttggcgca ggatttttgg ttgctgacgc agggcgatga gcgtagggcc
     7021 atcaatattg accgatgcca gcaggccgtg ctctataaag aagtcggctt tttgcgccag
     7081 caaatcaatc tgctctttca caacctccat ccgatggctg acggtgattt cagtaaaata
     7141 gcgatccggc ggcaggcgtt gcgaagggtt caagggatgc gtgaccaccg ttaatagctc
     7201 cacggccatt aaccgcccgc atgtttgata gatcggctgc caggtgtaag cacgctcaca
     7261 ctgcaaccaa aaacgccgtt cctgcaagct ctcgatgctt gcttcagggt tgcttattcg
     7321 ctggataacc tgccttatca tcaaagatgt cctgtgttta aaagtgatgc tgcccggact
     7381 cgacaaagat tatcggcgcg taagggcaga actttaccgc ctggccgggg taaatttcct
     7441 cactccacaa cggaatcttt caggaatacg ttctggctca aaaaaataat ggaacactgt
     7501 tttaatattg ttgaccagca aaccaccaca gcgcaaacta acgctaattt tttacagttc
     7561 aggttcacga ctatgtccaa aaagattgcc gtgattggcg aatgcatgat tgagctttcc
     7621 gagaaaggcg cggacgttaa gcgcggtttc ggcggcgata ccctgaacac ttccgtctat
     7681 atcgcccgtc aggtcgatcc tgcggcatta accgttcatt acgtaacggc gctgggaacg
     7741 gacagtttta gccagcagat gctggacgcc tggcacggcg agaacgttga tatttccctg
     7801 acccaacgga tggaaaaccg tctgccgggc ctttactaca ttgaaaccga cagcaccggc
     7861 gagcgtacgt tctactactg gcggaacgaa gccgccgcca aattctggct ggagagtgag
     7921 cagtctgcgg cgatttgcga agagctggcg aatttcgatt atctctacct gagcgggatt
     7981 agcctggcga tcttaagccc gaccagccgc gaaaagctgc tttccctgct gcgcgaatgc
     8041 cgcgccaacg gcggaaaagt gattttcgac aataactatc gtccgcgcct gtgggccagc
     8101 aaagaagaga cacagcaggt gtaccaacaa atgctggaat gcacggatat cgccttcctg
     8161 acgctggacg atgaagacgc gctgtggggt caacagccgg tggaagacgt cattgcgcgc
     8221 acccataacg cgggcgtgaa agaagtggtg gtgaaacgcg gggcggattc ttgcctggtg
     8281 tccattgctg gcgaagggtt agtggatgtt ccggcggtga aactgccgaa agaaaaagtg
     8341 atcgatacca ccgcagctgg cgactctttc agcgccggtt atctggcggt acgtttgacg
     8401 ggcggcagtg cggaaaacgc ggcgaaacgc gggcacctga ccgcaagtac cgttattcag
     8461 tatcgcggcg cgattatccc gcgtgaggcg atgccagcgt aaacgcagca tatgcaaaag
     8521 gctggataaa gtgttacccc tctatccagc tcatccataa atacctgatg gttgtgctaa
     8581 ctcatcacca tcaggcatta ctgtgcaggt ggaatatccg tcacttcagg atgtacgtca
     8641 tccgtggcga cagaggtggc ggcagcagca gtcgatgggg ccatgatttg atcccaggcc
     8701 gcctgcaacg ctttcatgtt aaattccggc tcgcctttcg gctgcagcag tattaacgcc
     8761 atatcattcg acaactgctg acgcagatcc tgatttaaca tctccaccgt caggctgtta
     8821 aggaaatcct gccgcagttt ctgatactgc tccggcgcga tatcgacgac ctgattttgc
     8881 aacgaacgca tccgctgacc catcagaata tcggtatcag cccgcgcata ggcggcaaac
     8941 agtttctgca gctccagttt cttttgcgcc actaacgcat tgaactcttc ttccggcaga
     9001 cctttatcgc gaaccttcgc cagttcacgc gccaccagat taaggttgct gttcagcttg
     9061 tcgtttggtg attcgatgtt gatggcacac tgcgcacgca gatacagcac acggcagtca
     9121 aatccaagac cgatgtcttt gctgttactg gcgcttaacg cttgctgaac atgccagaac
     9181 agtgcctcgc gggccaggtc cgcacgccag tagcgcagca gtgcggctga ttcacgaatc
     9241 ggctgccacg gcgtatccca catgatagat aaccggtcct gacgcaccgc gtcagtcata
     9301 atactcaccg cttccgcacg cagcggagaa agcgtcggca ccggagctgg cgtttcacgt
     9361 ttgcctttca gttcgccaaa cgttttgttg atttggtcga caaccgagcg cgcatccacg
     9421 tttcccacca ccagtagcgt cattgcatcc ggggtgtacc atttctgata gaaatcttta
     9481 attttttccg cttcaacggg ttgtttcagc ggatcggcag gatcgtgacc taacaaggtt
     9541 gaccctttca gacgatagcg ccaccagccc tctttagtat cggcaggcca ggttgccacc
     9601 atgtcctgac tttgcagcgc gtggttgatg gtttctggtg tgatggtcaa tttgccagtg
     9661 gcatttgcca gataagagag cgcttctttc agcaaatcgt tacggttatt gggcaaactc
     9721 agattaaaca gcgtggtgtc ataagagaca attaccggcg gcatcgggcg tttagggtcg
     9781 atcccctgct gccacaatga acgcgcctgt gctgcgtcaa ggccaccgct ttgcgttagc
     9841 gcaatacgag ggatggcgtg actgtaaccg ctctgttgtg tactttcggc gagcgaaccg
     9901 gtattaacca gcaggcgaat ttcaacacga tcgctgggac gctggggggt ggtcagcact
     9961 tgccactgta aaccgttgga aagcgtcccc tgttgccatg ctggatcagg ctggagcgca
    10021 tctgcctgca catagccagc agtggccatc atcagcaaac cgcccgctaa aagtcgaatt
    10081 tttgtgccct gcatgtgaac ccctgatcaa ctatcctggt aataaaaaaa ctgcccgttg
    10141 tcagtcagtc taaatgacgt taaaaacact tcgtgttaga ccgcaagaac atgaaaatgt
    10201 cacggaagaa gtgaaataaa cctgaactca cccaggtggt aagttcaggc acaggggtca
    10261 attatgcgca aacacccgca ctcggggaag ggagtgcggg cataagtggt gagattaaga
    10321 ggataattcg tgcgttttgc catccggcgc acgattattc agcacatcgt ccagtttttt
    10381 gtggtccagt tctttcaccc acttagcaac gacaatggtc gctacgccgt taccgaccag
    10441 gttagtcagc gcacgagctt ctgacataaa gcggtcgata ccgaggatca gcgccagacc
    10501 cgctaccggc aaatggccca ccgcagagag cgtcgccgcc agcacgataa agccactacc
    10561 cgttacccct gccgcccctt tagaagaaag cagcaacacg attaacagcg tgatttggtg
    10621 gacgatatcc atctgactgt tagtggcctg ggcgataaac accgccgcca ttgtcaggta
    10681 tatcgatgtg ccatcaaggt taaacgagta gcctgtcggg atgaccagcc ccaccaccga
    10741 tttgcggcag ccgagtttct ccatcttgtc gagcatacgc ggcagcgccg actcggaaga
    10801 tgaagtcccc agtacaatca gcagttcttc acggatgtag cggataaatt tgaagatact
    10861 gaaaccagtc gctttagcga ttgaacccaa taccagcacc acaaacagga tacaggtaat
    10921 atagaaacag ataatcagct gccccagttg caccagtgtg ccgacgccgt atttaccgat
    10981 ggtaaacgcc attgccccga acgcaccaat aggtgccaga cgcatgatca tattgatgat
    11041 gccgaagatg acctgcgaga aactttcgat cacgttaaaa ataagttggc ctttgctgcc
    11101 cagacggtgg agcgcaaaac caaacagtac ggcaaacagc agcacctgca gaatgttacc
    11161 gctggcaaat gcgccaatga cgctcgccgg aatgacatcc ataatgaagg cgacaatgcc
    11221 ctggtctttc gcctgatcgg cgtaaaccgc taccgctttc gcatcaagcg ttgccggatc
    11281 gacgttcatt ccggcaccag gctgcacgac gttaacgatg ataagaccaa taatcagcgc
    11341 gatggtactg acaatttcaa agtaaagcag tgcgactgcg ccggtacgac cgaccgcctt
    11401 catgctttcc atgcccgcaa tgcccgttac gacggtacaa aagatgacag gagcgatgat
    11461 catcttaatg agcttaacga agccgtcgcc aagcggtttc atttgctcgc ctatttcagg
    11521 atagaaatgg ccaaggagaa taccaatggc tatcgctgtc aggacctgaa agtaaaggct
    11581 tttaaacaga gaggttttca tagggtgtcc tttagtaaaa ctacaggtct tgtaaggtta
    11641 tgaggtacct gcggccttaa aataacaccc agacaacatc acagaaatgt acctggatca
    11701 taattgaaac aaaaaggtta aaaagtttga gctggctcgc acaaaccagc actttttaaa
    11761 gttttgtaat cagtttgggg tagctacttt tcttccaggt aactctcttc gaagatttca
    11821 atagggagtg ggcgagcaaa aaggaagccc tgggcaatac caacgcccgc ttttgccagc
    11881 cagtcgcgtt gtgcttcagt ctccacgcct tcggcaatca tttgtaagtt caggctctgc
    11941 gccagcatga taattgcagc aatcatgctg ctatcttccg gcaagccttc aacaaacatt
    12001 ttgtcgattt tcagtacgtc gattggcaac gatttcatat gctgcagctg acgcagccct
    12061 gcgtagccca tgccgaaatc atccagcgcc acccgaactc cggcattgcg cagcggacgg
    12121 aggattgcca ccgcagcatg agggtcgtca atacgtcggc tttctgtcac ttccagaatc
    12181 agtgttcccg gctgaatgcg atagcgggtt aacagttcca gcatatccgc caccatattt
    12241 gggtgcatca gttgcagcgc agagaggttt accgacaaga gcagcataat gccgcgctct
    12301 tgccaggctg caagcaatcg acaggactct tccagcaccc agtgaccgac ggtaaccatc
    12361 agcccacagc actcaatgcg atcgattaag ccatccggca ggtcccaact gccatccggt
    12421 tgctggatac gcagtaacac ttccgcactg accagtttac cgctggtcat ctcgacctgt
    12481 ggctgtaacc aaatagcaaa ctgatgattt tccagtgcat taaggatatc gctctcttcc
    12541 gtcaaccgct tctgggcggc ttccatctgc tgcggatcaa agaactgaat ctgattcttg
    12601 cctttatggc gagcggtaaa tgccgcagaa atagcgcgac tgtaaagctg ttcggcggtg
    12661 agatcgccgt agaacatcgc cacgccaatg ctacagtgcg gacggagttg aatacgttca
    12721 atcggcaggc gctcgctcat gatagtgagc acttgctgac ctaaggtgat tgcgtgccac
    12781 ggttcctgta caccgttggc aatgacagca aagtcataac cgctaatctg cgcgaggatc
    12841 atacgtggcg atagtaccga tttgagtttt tccaccagcg tcagcagcag aatttctcgt
    12901 tgcgcctctt tcagcacgcc cgcagtatca cgcagggttt cacaggtgat gatcatcagc
    12961 gcggtggttt gtttacgcgc gacaacctgc tccagcatct ccatcagcaa ggctttgttc
    13021 ggcaaatccg acaccgggaa gcgcatcgca ttttcgttct gttcttcata atggcgctgc
    13081 agcaattgct ggttgaggtt gtaactgcgc accaacatac cgatttcatc gtcctgatgc
    13141 agacgcggta atgccagttg gtgaccaaca agctcctggg ctgggatggc gttaagttcg
    13201 caagcaatat tgcgtaacgg atgcaaaatc aggcggttaa tgcaccagct gatggcgacg
    13261 gtcaggataa gcgacaaaag taagtaaatg gtcactaacg ttgagagggt gctcatcacg
    13321 aacttataca tacggaagga atccgcctgt agtaccagat aggcaattgg ctgcgggttt
    13381 gccggacgtt cgagcgagta aacgcccagc gagatttgaa ccggtagctc aaacaggcga
    13441 gtaaccatta ccggcaccgg gcgctctgga ataaaacttt tacgcagcgc ctggaactgg
    13501 ttaggcagca ctacatcggc acggctgacc acgcctgccg gtttaatgct ggcaagaata
    13561 gcttccgctt cgggaatatc gcctttcaaa atagctgaag ataagggttc gcgtacagag
    13621 cgagcaatgc tttccagttg cgtagccgtg ttatagcgat tctgctggac cagatggaac
    13681 agcaaaacgg tgcaaaaaat aaaaacgaac accaggacaa cggctgccac cattgccatc
    13741 tgcttgattg ttaacgagcg acttacgcgc aaattgtctc cccccggaaa tcccagattg
    13801 ccgcccgagt atacccgatc gcagcggcat taagagaggc gctatctgaa aacttaccag
    13861 ccggcgtaag gtatcagcgg ctgcggcggt aaatccatgt caccctgcca tccggcggcg
    13921 gaataacgta cgtagagcag gaaatggctg ggtgcgtaat ctttcgcctg ctggatatca
    13981 attgccgtgc caacaaacca gttggaagta acacgtcgtt caagtaatgc ccgcgccgtg
    14041 tagccgaagc cctgactgct gccgccatcg ttggattgcc gcgcagcttc ttcctgccag
    14101 tcggtcggga tcagattcat cagcggataa cgcggcatgg tttggtgcgt gaatgcgacc
    14161 acgagccaga cgcccccagc tcccacgacc agttttccgt gcgctcccgc cacatcaccg
    14221 gtatagcaaa cgacaggtat tcctgcggac tgtagtaacc gccctgaccg agtgagtagc
    14281 cactcagatc tttgtcgtaa tgccagatca tgttgttcag gccgattgtg acgcggcgat
    14341 tgttctggtt aatgacctta tagtaatagc ccgtcatcca gcgcacgcgc cagttatctt
    14401 cgacattttt accggttaac tggtcgccac taagcgatgc ccagacgccg tttgcttcac
    14461 ctttatcgta gctcagactt agccccacac cgtcggcacg tacgccaccc cattttttcc
    14521 cggtattgct cggggagtct ttttgcccac caaaggccag caaagaactg gagatgggcc
    14581 gacggtgggc gttaacggtg taacccagcg gcccgatatc atcgctgtaa ctgatgccgc
    14641 cgaccacatc caccacgttg aagcccatcg gcgtggtacc gatatcccag ctccagacgt
    14701 catttcgcca gccgaccgcc acgctggcac cggagtccga ctggctgcgg ttgccactac
    14761 agtcctgtaa tgtacaggtg ccccagttgt catcccattt gccatcggca ttagtggaga
    14821 aactgccgac gttcatattg acgaaatcac tgcgaaagaa catccgcccg tcagaatacg
    14881 gcgcatccac ctgcaacatg gtagtgtgcg ctttcagatc ggagtaacca ccggtgccgc
    14941 tcgaacccca gtaatcgtgc tcgagggtga cgttaagatc ctgctggcga tagaggcccg
    15001 ccgcatcgct gcgcacgccg cgtttcagcc agtcatcttt ctcgtcgtta cgggtcagtc
    15061 gggtgaatgt gtcgttatcc tgcggacgcg tcgtagtcac accggatgcg accatggcgt
    15121 ctttgtaggt ttccagcgcc tgctttggat cgcccgcctg cgcttcaaac ttcgcaccgt
    15181 cgcgcagcac catcgcgctt tccatcgacg gtggctgaga ttttgcctgc gggatcaact
    15241 tattaaacgt ctgctgcgcc gctgcggtat ctccaagctg cgcctgcgcc tgcgcctgcg
    15301 cctgcgcctg cgccagcgcc acgcgccgct gtgtgttcag cgaggcgtta tcggtagcgg
    15361 gcagcttcgc cagctggcta cgtgccgccg ctgtgtcacc ggcagcaata tccacttccg
    15421 tcagccccag aatggcatcg gcgttagttg gccccagcgt caggacattc tgatatgcag
    15481 cgcgggcggc ggtgtaatca cgtcgctgct gcgcccagtc agccagcgtg aggtcaatac
    15541 gcgtggaagg tggttgctga cgcagcatcg cttccgcttc cgcttcagct tctttgccgc
    15601 tttctcgcag gcgattagcg gtttccagca cctgatcgct ttgcagtcga ttaaccagct
    15661 cctgaatatt gctgttccac tgcgcgcgcg gcaggctgtt gatatgcgcc agcgccgctc
    15721 tgtcctggtc atgaccagag aggtacagcc cgtaagcgta aacctgctcc gggtcgttcg
    15781 gcttctgctg cgccagattg cgcattaacg tatcggcctg gctgcgttgt ccggcctgcc
    15841 agagatcctg cgaaagtcgg taagtaatcc atacgctgcc ggggtccagc gccagtcgtt
    15901 gccgctgaag tgctgccgcc tgcgcccatt tgccctggtt ttccagtgcc tctgcctgct
    15961 gtgccagacg gtcgttttgc aggctgcgtt cgatatcatc aatgctacgc cgctgactgg
    16021 cagagagcga ggcgataaac gcttcagctt tttctggcga ttgctggcgg taagtatttg
    16081 ccagcccgcg cacggcgtta gtgttgccgc tgtccatacg caaggtctgc tgataataac
    16141 gttcggcggc gggataatct tttcgcgcca tcgccacatc gcccagcccc agcactgcat
    16201 aactgtcggt gttatcgaca ttacgcgcct gctggaacag gcgttctgcc cggtcaggat
    16261 tattggcttt cagcgcagca tcgccctgct ggatcgccag ccagtagcgg tttactttca
    16321 gcagactgtt ccatttgtcg ttgttgctgc tgtgcgggtc cagtgcgagg gctttttcca
    16381 gattcgccac tgcattggcg cgatcgcctt tctgagaata cgcctggccc agcgccccca
    16441 gagcttcact gtctttcggg ttcgcccgca ccgcctgttg tagttcggga atggctttac
    16501 ccgccatacc agagtccacc gccgctaaac cttgcgcacg agcgcggaaa gcaggatcgg
    16561 ccagctgttt ttgctgttct gccagttgcg attgcgcagc cgccacgcta tcgccatcac
    16621 taaagatcga gagatatttt ttcagcgccg acacactggc atcactgacg ggcatgtctt
    16681 taatctgccc gtaccagatt ttagaggccc cttcgcgccc ggcgttcgat tttgccatct
    16741 gttccaggac ggcaaaacct tcgtcacggc gatcgctact aaacagcaat agcgccagat
    16801 tgttttgcag gcccgtattg cccggtgcat ccgcattgat gcgttttaac tgattaatcg
    16861 cttcgccacg gcgagccgga attttcgcca ccgtactcca gtactcgaca gcaatgtcac
    16921 cttccggcgg cgcaccgttg aacagtttgt tgtaactcgc cacagcttct tctgcatgac
    16981 cggtcgtcgc ctgcaatcgt gcctgttgca gtgcctgacg accatccggc gtggaaagta
    17041 gcatcgtagt ccgcgacgat ttatacgcat ttgaactcgg cgctaactgc gacagccgat
    17101 cgagctgttt ttgcgcgcca tcaatatcgc cctgacgtaa caaagaacgg aaacgggcgg
    17161 caacgacgtc cgggttattc ggatcaataa gttccagccg atataacgac tgttgcacca
    17221 gatcttcacg atgggtcgct tcgcctaacc gaacttgctc cagcaactgt tgctgagcgg
    17281 ttggtgctgc ctcgaccatc ggcatgacgg ccagaccgag ggaaagcgtg aatatgttta
    17341 gtgtgaattt gcgcattcct ggccccagtc aggtaataac tcaccttttg tcgagaagcg
    17401 gaaacggtgt tgatcccagc cttgtccaaa cagggtcagc acatagttgt aataggcatc
    17461 gctgccggga aagttatcgg ccacgcgctg gcgctgaacg gcctgcgcat cgcggttttg
    17521 taaaaagggc agcatggcgg cagaaaaacc gactggtcct ttaccctgcg ctttccccgt
    17581 agccacatcc actttttccg gcggataacc gtttttctca gtgaatgtcg ccatcggttt
    17641 aaaccggttg agcatccgcg ctttctgcgg atcgctgtca ggcatcatgc ctacccacat
    17701 gtaaacgcgg atggcgtcgt agctgctgat caatgttttt tcggctttta gctgccagcc
    17761 tttgtctttc tcataacgca cccagtctgg cgaaaagcct ttcggggcgg tttccagcaa
    17821 taaacgttga ttggtttcgc gcagcgtagt ccacggcgcg ccaaagcggg tgaaatactg
    17881 cgccagcgtc ggcggcaggt agctggggtt aaaacgccag ctgttatcct cagcaaaacc
    17941 cactttgccc ggtaacaaca tggaacccag cccaggcacc gtcaccactt cctcccgcgc
    18001 gatacgtttt agcaacgcgc tgccgatgtc ggtataacgc tgctctttcc acaaacgccc
    18061 cgcctccagc aacgaccagg ccatccagac atcaccatcg gaggccgaat tgctgtccag
    18121 cacttcccac ttactgttct ctttcttgcc ccacagccag gcgggcaaac gttcttttaa
    18181 agaaccctga gcgagattgt tctgcgtcca gtcgagaata ttatcgaaag ctgcacggtc
    18241 gttagccgcc agggcaaaga acataccgta actttgccct tcggaggtgg tgattttgcg
    18301 cgcgtcgctg gggtcgatga cgcgcccttc ctgactgatg taatcctttt taaactgctc
    18361 ccaggcaggc caggtacagg ctgcctgaac actaaaggca gccaacaaca gcatcgtcac
    18421 gattccacta cgcaacacat tcatcttcaa ttactcgtta tccgggttaa gacgacgacg
    18481 actaataatt cgcagcagac gccacagtac ccatgccagc aatatcacac tgatagccgc
    18541 cagcaccgcc agcagaatcg gatggtttgc cagcgcatac cacacgcgct cgaaccacgg
    18601 cagatgacct acgtaataaa cgtcgccaac acgtaggctg ttgataccgg actcgcggat
    18661 caccgcgacc gaaccgaaca tggtggcgcg tttgccgcta tcgttcaccg catcgttaag
    18721 catttcataa ccgcgtgggc tatctgccaa cagcgcaatc acgctgcgct ggtcgttata
    18781 cggcgactgg aagccaatca ccgccgccat cgcaccggaa gaggtcagcg ttgaccgggt
    18841 ttctgccgcg cgatcgctct cgtccggcac aatgccgggg aacggggtct ggcgcatcgg
    18901 tgttttcacc cagctttcgg tcgcctgcac caataggtcg atctgcttat cgtctttcag
    18961 tttgtccggg ataccaccga tgatcatgat gtcggcatct ttgccctgaa tggtgctgcc
    19021 atcatcggtc accgtcagat taatcgccgg gaagcccgtc tgtgcgccga taaaaccaac
    19081 agtattcagc aacgtttcca tctgtgcttc gttaggcgct ttcggcatca cggtgatggt
    19141 ttgcgacaga tccgccatcc ggctgaatgg gaagcccgcg ttagcaaagg cgcgtagatc
    19201 cggcatcggg atgaagtggt aatacttcga gaagtcgatg gtggagtcgt caccaatcac
    19261 cacatgattc tgcaccggct ggaaggtaat acagttatcc accgaaccgc ccggcatcgg
    19321 gttcatatac tcaaagtcga agcgcagctg gttggtcgcg cccagtttca gcgccggaat
    19381 agagacatct gttttgccat ccagcaaacc ttgtaatacc ggaatccgca gcagcaggcg
    19441 gttcgcctcc tgtttgctgc tcaggttgaa ggattgcagg aactggttat tcaggctgat
    19501 atccatccgc gaactgtctt tcaccggcgg catggtgtag cggtaattaa tatccatatc
    19561 aatgccggta ctgcgcatca ggtagagatc cggcggcagg tttagcgaaa cgttaatcgc
    19621 tgctggctca agaccgctgg attgtaactg ttcttcatag gttttcagtt cgccaaatgt
    19681 gaccggacga tcggtacgta cccagttcgg cgcatcgtac ggcttacgcg gtagcagcgg
    19741 tttcacttca ttcactacca cgctttcacc acggaacaga atgttaccct gagcgatacc
    19801 tttcgctgcc tgcaacaggt ctttgtcgtc acgaccaaac accaccagca gtttgacgta
    19861 aggattctgc ggatggttga tcatctcaat caccggggct tttaccgccg gatgatcgcg
    19921 caggaagtcc ggccgtttgt cgttggttgc aaagacaatg gcattacgat ccggcagttg
    19981 gttatagagc accgggaagt tctgcccacg ccagccagaa cgcgaaccaa accacgaggc
    20041 gacaatggca gatgcttgtt gcagcccaac atccggcgca cccgcaaaga ccatcggcaa
    20101 ggtgttagta cggttatcgc gcgggtcaaa gaatggcacc gggaagtgtg acaggtcatt
    20161 cttcacattc agggtctgat aggtcagatc cagtccactg ctccgcccaa catccagcca
    20221 aagcgtggtg ctggccgggt tttcgcacac gtcctgataa tggccgacaa actccagccg
    20281 cacacggttg aagtcggtaa taaacagtgg gttaatgggc atttgcgcca gcgttttttt
    20341 acccaactgt tctttggtca ctggcagtac gcccatcagc tcatcattga gataaacctt
    20401 taactgcgac tggacaggca gtaacgatgg cgatggcatg tattcgaggt tgagcatcgc
    20461 cttcgtcacc acttcatcgc tgcgcatacc aaactcaatg ctgccgttcg ggttaatgcc
    20521 acgtagcacc atgctgcccg gcggcggcgc aatttgcgca aaggtcagct tcacatcacg
    20581 cgaaggaccg ttctgcgcca cgactggcgc atcagcgccc tgcacgcccg gcatcacttg
    20641 ccccacctgc ggattttgtt ccgtctgggc gactacagct ggctcagcat tgatcagtgg
    20701 ttgcgttgct ggcgtcgcct gcgtcatgaa agaggggaac gcactcatcc ccatagccac
    20761 tgcacaaatc cagaatagtt ttcttttcat cgcgttatca tcattgttga gccaaagcct
    20821 gatccgatgg ttgtgccgtt tcgctccgct ccgggcggcg cagaataaac gatacaaccc
    20881 aggaaaccag agaagtcagc acacggaata tgcccttcac cgaagaaggc gcaaactccg
    20941 ccagatggcg gtagccacgg aagccgagct tcagaatatc cagcagactt tccagcggct
    21001 tatcttccgg gtagctgtcc tgccagagcg cccatgtatc cgcacgggca aacgtacact
    21061 gcacaaaatc gatatgttgc tgggtggtga gcggcattaa tttcagccca acttcattac
    21121 ccatcacgcg cgccacctgg gtcgggaaga cgtattcctg ctgaccgcgt ttaagcaaca
    21181 gattcacttt ctgcccttcc agaatctgcg cctgaccgtt gatcttgatc cccaaaccac
    21241 cgtcggagaa atcctgaacg gtacacgaaa agaggtgacc atcttcgcgg gcaattgccg
    21301 cgggcatcgt catctccacg cggtgcgatc ggcgtacctg tttgctttct accgataccg
    21361 caactgcgcc gccaagaaca atcaggttgt agaacaccca caccatactg acgaccacgg
    21421 tgagcatctc ggttggcggg ccatagaagt agcgccagat gccgaccgca acacccacca
    21481 ggttgagcag aacaaggaag atgtagggcc gcgagatcac ccagtcgacg tactcttctt
    21541 ccaccagtcc acctttggcg gtgacgttaa atttgccttt gtgcgggtta atcagcgcca
    21601 ccagcgtcgg tggtgcgata taccacgcca gcaccgtttc gtagatttca ctccagaaag
    21661 agtggcgata tttgccctgg atcttcgagt tggtcaggct ggcatggatc atatgcggca
    21721 gcacgaacag ggcgatcatc aacgctggcg catagatgat gtaggcatga agcagcagga
    21781 acgccagcgg cgcagtcagg aagatcagtc gtggaatgcc cgacaagaag tggaacatgg
    21841 cgttgacgta gcacagccgc tgggcgaatt tcagcccttt accggtaagc gggttatcga
    21901 gacggaagat ttgcaccatc ccgcgtgccc agcgaatacg ctgaccgata tgcgccgaca
    21961 gactttcggt cgccagcccc gccgcctgcg gaatacgcat atatgcagag gtgtagccac
    22021 gacggtgcag gcgcagagac gtgtgcgcat cttcggttac cgtttcgaca gcaataccgc
    22081 caatttcatc caatggtttg cgacgaatta ctgcacagga accgcagaag aaagtggcgt
    22141 cccacatatc gttgccatcc tgcaccagac catagaacag cgtgccttcg ttcggcgttt
    22201 tacgaaaacg ccccaggttg cgttcaaacg ggtccggcga gaagaagtgg tgcggcgtct
    22261 gcatcatcgc cagctgtttc tctttcagga accagcccat ggtcatttgc aggaacgatc
    22321 gcgttggtac gtggtcgcag tcgaaaatcg atacgaactc gcctttggca tatttcagcg
    22381 cattgttgat gttgcccgct ttcgcatgtt catgagtggt gcgggcgata tatttcaccc
    22441 ccacgttttg cgcaaactgg cgaaactctt ccctgccgcc gtcatcgagg atccagatgt
    22501 tcagtttgtc tttcggccag tcgataccca gcgaggcgta aatggtattt ttcaccacgt
    22561 tgagatcttc gttgtaagtc gggacaaaga tatccaccga cggccacagc gacatatctt
    22621 tcggcaatgg caccggctga cgattcagcg gccatacgac ctggaagtag ccgagcacca
    22681 gcacaatcca cgcgtacgtt tcagcgaaga gcagaataag cccgcacacc aggctgaccg
    22741 gatcgtccca gttcagcgta gaggtgtaac gccaccagat ataacggcaa gaaacggtca
    22801 gcgacagcac aatcaacatt agcgccgaga agcgccccgg catccgccgt acgatcagcg
    22861 ctacacccca cagcagcatc aggaagataa actgcgccag cgggttaaac ggttgagtaa
    22921 cgcagattaa cgccagaatc agcgagaagg tgacgatgat accgaggatc aaccgccgcg
    22981 ccccggcact caaatgaccg agctcttttt tctcatcaag atgctgtgtt ttatgactaa
    23041 cgcgctcagg cagctcgttc atccattgat ggtaacgtcc acgaatattc tgcagacctg
    23101 aaaatgcccg cctgcgcggt ttcggcgttt ctttgcgcga tgtaccgatc agtaaccagc
    23161 atgtttgaat gaaataacgg accgggtcca gcggacgcgg acgcgaggcg ttgatatgcg
    23221 gatacaggtt tttatgttct gcgcgaatac gctgccagcg cgggtgctcc agcggaataa
    23281 aaatccaggc caggatcatc cagaaacagc cgagcgtcgc gctgaaagcc gacgcaccgt
    23341 gacgacgata atcgcgataa cgcccgataa gccgcgcgtt gaccggcggg ataagcaacc
    23401 accgggtcag gatactcatg atgcactccc gactggcgtt ttcagcccgg agtagttcaa
    23461 caggcaccag ttcgccagcg tcagtatctc ttcagccgcc agcgcatcac tgcgatattc
    23521 acctactggt tgcttagccg ccaggcattc agccatcgct tcatcacgat gaatgagcat
    23581 cggcagtaat cggcgctggc tttgcaacca aagctggtaa atatcgtcct gaacctgact
    23641 gccaatacgg aagtcattaa tcaaaatatg tgcgccatcc ggcagcgctt gctgatgcag
    23701 tcggatatgg cagttggcat ccacattgac gattgccagc gagtgatcgc acaaactcag
    23761 cagctggtgg gttatctgcg aggcatcacg cggtaagtcg attaaaatcc acaggtaacg
    23821 cccgctggct tttagttgct gtaagccgga gcaaatatcg ctcagccggg tttgccagtg
    23881 ctgtggattt tcttgttctt caatggataa ctgaccaaaa ggcagcaaat cgagctgcga
    23941 ggtgtagcgc aacccagcgt cacgccagtc ctggccatcc agcatcgctc tggcccagcc
    24001 ctgacggtgg gtaaaatcaa cattaaatga caggcgcaac aagttgtccg ggcaggcatc
    24061 gaccaccagg acattttctc ccaacatttg taatgaccag gctaatgcgg cggtgatggt
    24121 tgttgtcccc acgcctcccc gcaccccctg caatcccagt acggccattc accaatcccc
    24181 tacttttgtt gcgcaaactc tgccagcaac ggccagcgtt ttaatgccgc ggccaactgt
    24241 tcgcgttggg aaatatcggc ataatcaata tcaggcagtg aaaatgcctg ctttaacgcc
    24301 acaatatcat tctggaagat atagcctatc gcgggatcag gcagagtatc tggttcattg
    24361 ttattcattt tgcttgatcc ttatgaaata accgtaccgt tcatgagaca attctaaccg
    24421 tgagcatttc gccggtttat tgtttacggc gagacgggaa tttgctcact ttttgacttt
    24481 ccgttacgca acagaaaaag ttaaaaacga aaagactaaa atcacacgtc ttgtgacctt
    24541 ctaaattgat tcacatgcta aatctgataa gttttaattt caatggtagg tttatttctt
    24601 agctttcgct agtaaactga taatcagaca aaatggtgac atgagggaca ttgtgggccc
    24661 tgtattctct atcggtatct catcattatg ggatgagctg cgacatatgc cagcaggcgg
    24721 cgtctggtgg tttaacgtcg atcgccatga agatgctatc agtctggcga atcaaacaat
    24781 tgcatcccag gctgaaaccg cacacgtcgc ggtcattagc atggacagcg atccggcgaa
    24841 aatctttcaa ttagatgatt atcaagggcc ggaaaaaata aaattatttt caatgctaaa
    24901 tcatgaaaaa ggtctatact atttgacccg tgatttgcag tgttctattg atccccataa
    24961 ttaccttttt attcttgttt gcgcaaataa cgcatggcaa aacattcctg ccgagcggct
    25021 tcgctcatgg ttggataaaa tgaataaatg gagcaggtta aaccattgtt cgcttttggt
    25081 aattaatccc ggaaataata acgataaaca attttcattg ttgcttgagg aataccgttc
    25141 actttttggt cttgccagtt tgcgttttca gggtgaccaa catttgctgg atattgcctt
    25201 ctggtgcaac gaaaaagggg tcagcgcccg tcagcagctt agcgttcagc aacaaaatgg
    25261 tatctggaca ttagttcaaa gcgaagaggc ggagatccaa ccacgcagcg acgaaaaacg
    25321 cattctgagt aatgttgctg tactggaagg tgcgccgccg ctatcggaac actggcaact
    25381 gttcaacaat aacgaagtcc tgttcaatga agcccgtacc gctcaggcgg cgacggtggt
    25441 cttttcttta cagcaaaatg cgcaaatcga gccactggcc cgcagcattc ataccctgcg
    25501 tcgccagcgc ggtagtgcga tgaaaatcct cgtgcgggaa aataccgcta gcctgcgcgc
    25561 caccgatgaa cgtttgttat tggcctgcgg tgcaaatatg gttattccgt ggaatgcgcc
    25621 actctcccgt tgtctgacga tgatcgaaag cgtgcaaggg cagaagttta gtcgctatgt
    25681 gccggaagat atcactacct tgctgtcaat gacccagccg ctcaaactgc gtggtttcca
    25741 gaagtgggat gtgttctgta atgccgtcaa caacatgatg aataaccctc tattacctgc
    25801 ccacggtaaa ggcgttctgg ttgccctacg tccggtaccg ggtatccgcg ttgaacaagc
    25861 cctgacgctg tgtcgcccta accgtaccgg cgatatcatg accattggcg gtaatcggct
    25921 ggtgctgttt ctctcattct gtcggattaa cgatctggat accgcgttga atcatatttt
    25981 cccattgcct actggcgaca ttttctcaaa ccgtatggtc tggtttgaag atgatcaaat
    26041 cagtgccgag cacgatggtc gccactggcg acgaatacca gaacccatgc gactgttaga
    26101 tgatgctgtg gagcgctcat catgatgacc atcagcgata tcattgaaat tattgtcgtt
    26161 tgcgcactga tatttttccc gctgggctat ctggcgcggc actctttgcg acgcattcgc
    26221 gacaccttac gtttgttctt tgctaaacct cgttatgtta aaccggccgg gacattacgc
    26281 cgcacggaaa aagccagggc aaccaaaaaa tgactcaatt tacgcaaaat accgccatgc
    26341 cttcttccct ctggcaatac tggcgcggcc tttccggctg gaacttctat tttctggtta
    26401 agttcggcct gttgtgggcg ggatatctta acttccatcc gctcctcaat ttggtgtttg
    26461 ccgcgtttct gctgatgccc attccgcgct acagcctgca tcgcttgcgc cactggattg
    26521 ccctgccgat cggctttgct ttgttctggc atgacacctg gttgcctggc ccggaaagca
    26581 taatgagcca gggttcgcag gtggcggggt tcagtaccga ttatttaatc gaccttgtca
    26641 cacgctttat taactggcag atgat
//
LOCUS       DAJTNA010000040        26413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTNA010000040 DAJTNA010000000
VERSION     DAJTNA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26413
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.041"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(68..715)
                     /gene="ychE"
                     /locus_tag="OO161_001400"
     CDS             complement(68..715)
                     /gene="ychE"
                     /locus_tag="OO161_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5462533.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1193..3868
                     /gene="adhE"
                     /locus_tag="OO161_001401"
                     /gene_synonym="adhC"
     CDS             1193..3868
                     /gene="adhE"
                     /locus_tag="OO161_001401"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5462534.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVLKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYTDQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLLSELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3992..4888
                     /locus_tag="OO161_001402"
                     /pseudo
     CDS             3992..4888
                     /locus_tag="OO161_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5170..5787)
                     /gene="tdk"
                     /locus_tag="OO161_001403"
     CDS             complement(5170..5787)
                     /gene="tdk"
                     /locus_tag="OO161_001403"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5462535.1"
                     /translation="MVQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6383..6796
                     /gene="hns"
                     /locus_tag="OO161_001404"
     CDS             6383..6796
                     /gene="hns"
                     /locus_tag="OO161_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5462536.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6940..7848)
                     /gene="galU"
                     /locus_tag="OO161_001405"
     CDS             complement(6940..7848)
                     /gene="galU"
                     /locus_tag="OO161_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5462537.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8050..9063)
                     /gene="rssB"
                     /locus_tag="OO161_001406"
     CDS             complement(8050..9063)
                     /gene="rssB"
                     /locus_tag="OO161_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5462538.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9155..10060)
                     /gene="rssA"
                     /locus_tag="OO161_001407"
     CDS             complement(9155..10060)
                     /gene="rssA"
                     /locus_tag="OO161_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5462539.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            10173..10631
                     /locus_tag="OO161_001408"
     CDS             10173..10631
                     /locus_tag="OO161_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5462540.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFISFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10681..11523
                     /gene="purU"
                     /locus_tag="OO161_001409"
     CDS             10681..11523
                     /gene="purU"
                     /locus_tag="OO161_001409"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5462541.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSALLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11683..11767
                     /locus_tag="OO161_001410"
     tRNA            11683..11767
                     /locus_tag="OO161_001410"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11717..11719,aa:Tyr,seq:gta)
     gene            11802..11886
                     /locus_tag="OO161_001411"
     tRNA            11802..11886
                     /locus_tag="OO161_001411"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11836..11838,aa:Tyr,seq:gta)
     gene            11934..12065
                     /locus_tag="OO161_001412"
     ncRNA           11934..12065
                     /ncRNA_class="other"
                     /locus_tag="OO161_001412"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12249..12926)
                     /gene="narI"
                     /locus_tag="OO161_001413"
     CDS             complement(12249..12926)
                     /gene="narI"
                     /locus_tag="OO161_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5462542.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12926..13636)
                     /gene="narJ"
                     /locus_tag="OO161_001414"
     CDS             complement(12926..13636)
                     /gene="narJ"
                     /locus_tag="OO161_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5462543.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13633..15171)
                     /gene="narH"
                     /locus_tag="OO161_001415"
     CDS             complement(13633..15171)
                     /gene="narH"
                     /locus_tag="OO161_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5462544.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15189..18911)
                     /locus_tag="OO161_001416"
     CDS             complement(15189..18911)
                     /locus_tag="OO161_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5462545.1"
                     /translation="MSKFLDRFRYFKQKDETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            complement(19427..20818)
                     /gene="narK"
                     /locus_tag="OO161_001417"
     CDS             complement(19427..20818)
                     /gene="narK"
                     /locus_tag="OO161_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5462546.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            21157..22953
                     /gene="narX"
                     /locus_tag="OO161_001418"
     CDS             21157..22953
                     /gene="narX"
                     /locus_tag="OO161_001418"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5462547.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            22946..23596
                     /gene="narL"
                     /locus_tag="OO161_001419"
     CDS             22946..23596
                     /gene="narL"
                     /locus_tag="OO161_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5462548.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(23597..24991)
                     /gene="ychO"
                     /locus_tag="OO161_001420"
     CDS             complement(23597..24991)
                     /gene="ychO"
                     /locus_tag="OO161_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5462549.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGRNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKSGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVAEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            25175..25528
                     /gene="ychN"
                     /locus_tag="OO161_001421"
     CDS             25175..25528
                     /gene="ychN"
                     /locus_tag="OO161_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5462550.1"
                     /translation="MQKIVIVANSAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(25572..26267)
                     /gene="chaC"
                     /locus_tag="OO161_001422"
     CDS             complement(25572..26267)
                     /gene="chaC"
                     /locus_tag="OO161_001422"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5462551.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
BASE COUNT         6720 a         6944 c         6380 g         6369 t
ORIGIN      
        1 gcgggaacaa agtaaaaata atgacgaagt aaacggagca tggcagctcc gtttcatttg
       61 aaaggaatta attaagcagg ccggggaaaa tccccttaat accagtaacg ataaattcaa
      121 tccccaatgc catcagcaat agccccataa tacgcgtaat cacgttgatg ccggtctggc
      181 gtaaaacccg taccagccaa ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca
      241 atgcaatagc cacaaagaaa ccaaacagat agctaatgct gtgataacgc gtaccccaga
      301 cgatggtaga actgatcgcc cctggccccg ccatcaacgg caacgccaat ggcaccacac
      361 caatgctttc acgtaccgcg gtttctgatt tttcttgctt gttctgttta tcctcgccaa
      421 gcttgccgct gatcatcgac atcgctatcg tcaccaccag gatgcccccg gcgatacgga
      481 acgaatcaat tgatatacca aaaagttgca gaatcgtgtc gccgagaaaa agcgagatcc
      541 acaagataat ggccacagac aggttggctg taaggttagt tttgtttcgc gctgccgctg
      601 tctgataact ggtcatgctg ataaagacgg gaataatccc taccgggttg accagcgcaa
      661 ataacccgat gaaaaatttg aagtaaacgg gaaaatcaaa aaaggtctga atcacggtta
      721 gctccgaagc aaaagccgga taatgttagc cataaataag gttgaaaaga cgcgctgaca
      781 atacgccttt tgacagcatt tttcacctcc taaatactta aaattgctat cattcgttat
      841 tgttatctag ttgtgcaaaa catgctaatg tagccaccaa atcatactac aatttattaa
      901 ctgttagcta taatggcgaa aagcgatgct gaaaggtgtc agctttgcaa aaaatttgat
      961 ttggatcacg taatcagtac ccagaagtga gtaatcttgc ttacgccacc tggaagtgac
     1021 gcattagaga taataactct aatgtttaaa ctcttttagt aaatcacagt gagtgtgagc
     1081 gcgagtaagc ttttgatttt cataggttaa gcaaatcatc accgcactga ctatactctc
     1141 gtattcgagc agatgattta ctaaaaaagt ttaacattat caggagagca ttatggctgt
     1201 tactaatgtc gctgaactta acgcactcgt agagcgtgta aagaaagccc agcgtgaata
     1261 tgccagtttc actcaagagc aagtagacaa aatcttccgc gccgccgctc tggctgctgc
     1321 agatgctcga atcccactcg cgaaaatggc cgttgccgaa tccggcatgg gtatcgtcga
     1381 agataaagtg ctcaaaaacc actttgcttc tgaatatatc tacaacgcct ataaagatga
     1441 aaaaacctgt ggtgttctgt ctgaagacga cacttttggt accatcacta tcgctgaacc
     1501 aatcggtatt atttgcggta tcgttccgac cactaacccg acttcaactg ctatcttcaa
     1561 atcgctgatc agtctgaaga cccgtaacgc cattatcttc tccccgcacc cgcgtgcaaa
     1621 agatgccacc aacaaagcgg ctgatatcgt tctgcaggct gctatcgctg ccggtgctcc
     1681 gaaagatctg atcggctgga tcgatcaacc ttctgttgaa ctgtctaacg cactgatgca
     1741 ccacccagac atcaacctga tcctcgcgac tggtggtccg ggcatggtta aagccgcata
     1801 cagctccggt aaaccagcta tcggtgtagg cgcgggcaac actccagttg ttatcgatga
     1861 aactgctgat atcaaacgtg cagttgcatc tgtactgatg tccaaaacct tcgacaacgg
     1921 cgtaatctgt gcttctgaac agtctgttgt tgttgttgac tctgtttatg acgctgtacg
     1981 tgaacgtttt gcaacccacg gcggctatct gttgcagggt aaagagctga aagctgttca
     2041 ggatgttatc ctgaaaaacg gtgcgctgaa cgcggctatc gttggtcagc cagcctataa
     2101 aattgctgaa ctggcaggct tctctgtacc agaaaacacc aagattctga tcggtgaagt
     2161 gaccgttgtt gatgaaagcg aaccgttcgc acatgaaaaa ctgtccccga ctctggcaat
     2221 gtaccgcgct aaagatttcg aagacgcggt agaaaaagca gagaaactgg ttgctatggg
     2281 cggtatcggt catacctctt gcctgtacac tgaccaggat aaccaaccgg ctcgcgtttc
     2341 ttacttcggt cagaaaatga aaacggctcg tatcctaatt aacaccccag cgtctcaggg
     2401 tggtatcggt gacctgtata acttcaaact cgcaccttcc ctgactctgg gttgtggttc
     2461 ttggggtggt aactccatct ctgaaaacgt tggtccgaaa cacctgatca acaagaaaac
     2521 cgttgctaag cgagctgaaa acatgttgtg gcacaaactt ccgaaatcta tctacttccg
     2581 ccgtggctcc ctgccaatcg cgctggatga agtgattact gatggccaca aacgtgcgct
     2641 catcgtgact gaccgcttcc tgttcaacaa tggttatact gatcagatca cttccgtact
     2701 gaaagcagca ggcgttgaaa ctgaagtctt cttcgaagta gaagcggacc cgaccctgag
     2761 catcgttcgt aaaggtgcag aactggcaaa ctccttcaaa ccagacgtga ttatcgcgct
     2821 gggtggtggt tccccgatgg acgccgcgaa gatcatgtgg gttatgtacg aacatccgga
     2881 aactcacttc gaagagctgg cgctgcgctt tatggatatc cgtaaacgta tctacaagtt
     2941 cccgaaaatg ggcgtgaaag cgaaaatgat cgctgtcacc accacttctg gtacaggttc
     3001 tgaagtcact ccgtttgcgg ttgtaactga cgacgctact ggtcagaaat atccgctggc
     3061 agactatgcg ctgactccgg atatggcgat tgtcgacgcc aacctggtta tggacatgcc
     3121 gaagtccctg tgtgctttcg gtggtctgga cgcagtaact cacgccatgg aagcttatgt
     3181 ttctgtactg gcatctgagt tctctgatgg tcaggctctg caggcactga aactgctgaa
     3241 agaatatctg ccagcgtcct accacgaagg gtctaaaaat ccggtagcgc gtgaacgtgt
     3301 tcacagtgca gcgactatcg cgggtatcgc gtttgcgaac gccttcctgg gtgtatgtca
     3361 ctcaatggcg cacaaactgg gttcccagtt ccatattccg cacggtctgg caaacgccct
     3421 gctgatttgt aacgttattc gctacaacgc gaacgacaac ccgaccaagc agactgcatt
     3481 cagccagtat gaccgtccgc aggctcgccg tcgttatgct gaaattgctg accacctggg
     3541 tctgagcgca ccgggcgacc gtactgctgc taagatcgag aaactgctgg catggctgga
     3601 aacgctgaaa gctgaactgg gtattccgaa atctatccgt gaagctggcg ttcaggaagc
     3661 agacttcctg gcgaacgtgg ataaactgtc tgaagatgca ttcgatgacc agtgcaccgg
     3721 cgctaacccg cgttacccgc tgctctccga gctgaaacag attctgctgg atacctacta
     3781 cggtcgtgat tatgtagaag gtgaaactgc agcgaagaaa gaagctgctc cggctaaagc
     3841 tgagaaaaaa gcgaaaaaat ccgcttaatc agtagcgctg tctggcaaca taaacggccc
     3901 cttctgggca atgccgatca gttaaggatt agttgaccga tccttaaact gaggcactat
     3961 aacggcttcc acaacaggga gccgttttct tatgccactt ctcaatgatc tgctcgattt
     4021 cagtgaccat ccgcttatgc ctccgccctc tgcacaacta tttgcagaac accttcccac
     4081 cgagtggata caacactgcc tgacgctttc tgctcatgcg accgttcgcc gccgtcgttt
     4141 accgggggac atggttatct ggatggtggt gcaatgagcc aattaccgat gttgttcgcc
     4201 gtctgaacct gagcgcggat ggcgaagcgg ggatgaacct gctggcccgc agcgctgtca
     4261 cccaagcgcg tcagcgcgtg ggggccgccc cagtggaatg gctcttccgc cagaccgcac
     4321 agacaggggc gcggaacgtt acctgaagga tgactggcac ggcctgcaac tttttgccat
     4381 tgatggcgca cagttcagga cacctgataa acccgagctg cgtgaatatt atgggtctgc
     4441 caacacatcc actaagcggc agaacgccta cccggttatg cgtctggtgg ccttaatgaa
     4501 cctgggaagt cacattctgc tgaatgccgt gaccgcacct taccggcaga gcgaaactgt
     4561 gctggcccac tccatgctcg ccaccatccc ggataactct attacgttgt ttgataagat
     4621 tttttacagc gaagacctgc tgctaacgct gaaccagaag ggttgtaacc gccactggct
     4681 actgcctgcg tggaagaata tcgcatcaga aatgatagag ctgggaaaca cggcgtcacc
     4741 agggaccatc ccgaaacgac tggagcatct tcggggtgct ctggaagtgg tgttcataac
     4801 aaaacgcccc cgaccatcaa ggccgaggtc ggttaagata tcaaaaaccc gttatcctgt
     4861 gaaacatagt gccgctcctc ttaagtgaac ggcattgccc ttctggggcc gtattttttg
     4921 tttacccaaa gcaacttttc cataaaccga cagcattagc cttcatcata tttgcgacga
     4981 tgtataacgc ctaaacacag ggatattgta ctttacaggt cacaagtcaa cgtcggtgct
     5041 taagagccct gtgaggcgta tagcggcgtt aaaaaactgc cgagaagggt atatagcccg
     5101 gaagaagtgc gtaaaacgaa ctgacaggat aaaagtgccc tgctcaccct gtcagtaaag
     5161 aaattcttat taatcgtggc gatgcctttc ctgaatagcc gttaatgagc cgacttgtaa
     5221 cgcctcttta tagtgtttac ggcatacaga aacgtatcgt tcattaccac caattaccac
     5281 ctgctcacct tcgttataag gtctgcctgc ttgatcaaga cgcagcacca tgcttgcttt
     5341 acggccacaa aaacagatgg tttttaattc aaccagtttg tcggaccatg ccagtaagta
     5401 ttggctgcca ataaataatt cacctcgaaa atcggtacgt aaaccataac aaagtacggg
     5461 tatatcgagt tgatcgacaa cctccgataa ttcatatact tgttgtctgg ttaaaaactg
     5521 gcattcatca accagtacgc aatgaattgc ctgctgttca tgttccgcac gaatctcatc
     5581 aaataatgat gaattttggt taaataattt tgcaggcgat gacaaaccta tacgcgaact
     5641 gactttcccg gcaccaaagc gatcatcaat ttctgccgta tatacgacag tgcgcatgcc
     5701 gcgttcttgg taattgtatg aagattgcaa caatgctgta gacttacccg cattcattgc
     5761 ggaatagtag aaatatagct gtaccatcag ccacaggccc tcaatgatat gaaataaagt
     5821 tggctggagt ttattcagtt tttcagctca acagtatgca cagaagtggg tatatgcgtt
     5881 ctcccttacg aagccttgca taatccttct gagctatcat tacaactgcc tcgcttgtta
     5941 taagcgggta aatgactgct ggtaactatt cacaaccttt aacctgttgc gcaagtaata
     6001 gccctctgtt gacctccagg agatagtgca atactaagtc catgctctta ttgcgactgt
     6061 tctacttttc atcattcgct taatagggaa ttctcgtaaa cacaactaat acagaagact
     6121 gaaaggtcgt cagcctacga taatctcccc ataaaatgtg acatgaatca ggaagtttta
     6181 acctcacgtg ctgcgaaatc atcggtgtaa atagggctat atgccgcgtc ttttctggct
     6241 aattttatga aaagatattt attggcggca caaaataaag aacaattttg aattccttac
     6301 attcctggct attgcacaac tgaatttaag gctctattat tacctcaaca aaccacccca
     6361 atataagttt gagattacta caatgagcga agcacttaaa attctgaaca acatccgtac
     6421 tcttcgtgcg caggcaagag aatgtacact tgaaacgctg gaagaaatgc tggaaaaatt
     6481 agaagttgtt gttaacgaac gtcgcgaaga agaaagcgcg gctgctgctg aagttgaaga
     6541 gcgcactcgt aaactgcagc aatatcgcga aatgctgatc gctgacggta ttgacccgaa
     6601 cgaactgctg aatagccttg ctgccgttaa atccggcacc aaagctaaac gtgctcagcg
     6661 tccggcaaaa tatagctatg ttgacgaaaa cggcgaaact aaaacctgga ctggccaggg
     6721 ccgtactcca gctgtaatca aaaaagcaat ggatgagcaa ggtaaatccc tcgacgattt
     6781 cctgatcaag caataatctt ttgtagattg caattgctta aaatcccgcc atcggcggga
     6841 ttttttattg tccggtttaa gacaatttaa taagaataag ctataaaaaa acggcgtcga
     6901 ttgttcaacg ccgtttcgtg gataacaccg atatggatgt tacttcttaa tgcccatctc
     6961 ttcttcaagc caggctttaa attccgtgcc aagggtgtta tgacgaatac cgtattcaac
     7021 gaaggcctgc atgtaaccta atttattacc gcagtcatgg ctcttccctt tcatatgata
     7081 ggcttccacc gtttcttttt cgatcagcat atcaattgcg tcggtgagct gaatttcatc
     7141 accagctccc ggaggggttt ttgccagcaa cggccaaata tccgcgctaa gtacgtaacg
     7201 acccacaata gcgagattag acggcgcaac atccgctttc ggtttttcta ccacaccaac
     7261 catcggtaca ctttcacccg gcgctaattc aacgcctttg caatccacaa cgccatatgc
     7321 ggtcacatca gcaacctgtt caaccatgat ctggctatga cccgtttcat caaagcggcg
     7381 gatcatctct gccaggttat cctgtgacaa atcggattca tattcatcca gaataacatc
     7441 aggcaaaata acagctaccg gttcatcacc cactaccggg tgagcacaca acaccgcgtg
     7501 tcccaggcct ttcgccagcc cctgacgaac ttgcataata gtcacgtgcg gcggacaaat
     7561 agactgcact tcatcaagca gttgacgttt tacacgtttt tccagcattg cttccagttc
     7621 aaaactggta tcaaagtggt tttcaatcga gtttttagat gagtgcgtaa ccagcacaat
     7681 ttcagtaatg ccagccgcaa tacattcatt cacgacgtat tgaattaatg gcttatcgac
     7741 aagtggcagc atctctttcg ggatggcttt cgtcgccggc aacatcctgg ttcctaatcc
     7801 cgcaacgggg ataacggctt ttttgacttt cgtattaatg gcagccattt aaattctcct
     7861 ggactgttcg tgttttgaac gtgttcatat ttctgtatcg catcccagta tatcagcacc
     7921 ccgaaggggc taagttctga aaaggaggcg agttagcata attaagacaa ttacttatac
     7981 gaatggcgac gatagtacca ccagccgaca ttagcaggta atgcaaattt agtccgcgtt
     8041 atcgtttgct cattctgcag acaacatcaa gcgcagtcga ccaccggttc cccatatttg
     8101 gcattgccag gcatcgcatc gctggctcaa ttgattcaaa taagcgttac ctaaagtgcc
     8161 taacggaaca ccattactga tttgcacctg gtgttcgccg gtatttaacg tcgcattcag
     8221 acccgcagaa accagaatga gatttttcag ttcgcgatga taatagccaa ctaatagcgg
     8281 aaactgcccc ggcagattgg cctgacggag taaatggttt acctgcttca ataacgcgcc
     8341 cagctctggc aaccgttgat tttggtgtgc aagctgttcc tgtaataatc cgttaaacaa
     8401 tgcgcgtaat aacaaggcag caagtacgcc attatgtcca gctcgggtga catcaaggca
     8461 ataaaatgcc agatcgtttt ccgaaagtgc ggcaatatca agcaccaggc cgggtttgtc
     8521 cgcggcaacc aattgacgat aattaacccg gcaatgggaa atcacctgct gaaccggcgg
     8581 ttgtagttcc tgtaataatt tcgccgctgc ggcagggtta tcaaccgttg catcccaatc
     8641 gcgaaaaagc ctttcctctt cctcaacgcg cgaattaaac atgctgggat agagacaggc
     8701 aaaaaccatc tcgcgcaagc gattcagatc tttaactggt ttcagcaaaa catcttcaac
     8761 gcccagacgt aacgctttgg caatatctgc catattttca gtggcagata tcaccagaac
     8821 tggggtctgg tcgcctctgt tacgtatatg ctccagcagt ttaagcccgt tcattcgtgg
     8881 catcgcgata tcacatatca tcaggtctgg agtgaaacct cccagcaact caagggcatc
     8941 caccccatca gccgccagta ccgttgtcgc tcccaatgag gaaaaccatg aatccagaag
     9001 cgagcgaaat acctgctcat cttcaacaat gagaatctgt tttccgacca atggctgcgt
     9061 catgttctct cccctgactg gctttactca atagtggcat gctattgcca cctgcgcctg
     9121 tcagaattaa cttaagtgta gataaaaatt ctggtcaaat gttggtgcgt accaacggca
     9181 aaagttcgtc cattttcctt tccactgcca gctgtccggc cgcaatggca gcgtgcgcgc
     9241 gatggaaatc aagcgtagat atttgcgggc aaacaggttg aatcagaata tcgggcggat
     9301 cacctgccat gcggttcctt ttaaggcggt tctccagcac ctggatagaa gtggtcataa
     9361 tctctgttgc cgttggcgct gtcaccgccc gacgtgttgt tatactgcct aacctttctt
     9421 tcagacgcgc atgccacggc aaagattcac cattctcgct attttcttca ctgacattaa
     9481 aggagagcaa atcttgttgc atcaaatgag catcgtgctg caggtcgacc gctatcacaa
     9541 tatcagcccc caatgcacgc gtgagggaaa taggaattgg gttaactact gctccatcaa
     9601 ccagccagta gccgttatgt gcaacaggtg ccatgagtcc tggaatactg catgatgcgc
     9661 gaatagcaag atggagatcg ccttcagtaa accataattc acgtcccgta cttaaattgg
     9721 tggcaacagc cgcaaagcga cgggaacaat tttcgatctc tgtttccggc attatttcgc
     9781 ggtattgatt gaagacacgc tcgccgcgca gtaacccacc gcgctgccag gagagatcca
     9841 tcaggcgtaa aacatcccaa taactgaaag aggtcaccca atcttccagc gcagataatc
     9901 gatcgcatgc ataggcagcg cccaccagcg aaccaattga acatcctgca acgatatcaa
     9961 tttcaatacc cacttttttt agcgcattaa taacgccaat atgcgaccaa cctctcgccg
    10021 cgccagatcc cagcgccagc cctattttta tctttctcat tattcccgca agattcccct
    10081 ggaatgcaat cgtagccaca tcgctactgc tcggttaaca tagtgctact caggcggtta
    10141 atacgccgtt attgtttcca gggagattat ttgtgtctca gctttgtccc tgtggtagtg
    10201 ctgtcgagta tagcctatgt tgccaccctt atgtgtctgg tgaaaaggtt gcacctgatc
    10261 cagaacatct catgcgttcg cgttactgcg cttttgtgat gcaagacgca gattatttaa
    10321 taaagacctg gcatccctct tgtggagcag cggcgttacg tgccgaattg atggccggat
    10381 ttgcgcatac tgaatggttg ggattgaccg tatttgaaca ttgttggcag gacgccgaca
    10441 atatcggatt tatcagtttt gttgctcgat ttaccgaagg tggcaaaaca ggtgcaataa
    10501 ttgaacgttc gcgatttttg aaagaaaacg gtcaatggta ctatattgac ggtacacgtc
    10561 cgcagtttgg tcgcaacgat ccctgccctt gtggttcagg taaaaaattt aaaaagtgct
    10621 gcggccaata atggttgacg gtacggttta gcaaacactc tcaacaaggt ttttccagca
    10681 atgcattcac tccaacgtaa agttctgcgt actatttgtc cggaccaaaa aggtctgatc
    10741 gcacgtatta ccaatatttg ctacaagcac gagttaaata tcgtacagaa caatgaattt
    10801 gttgatcacc gtaccgggcg cttttttatg cgcacggaac tggaagggat ttttaatgat
    10861 tccgccctgc tggcggatct cgatagcgca ttgccagaag gctccgtgcg tgagctgaat
    10921 cctgccggtc gtcgccggat agtgattctg gtcactaaag aagcgcattg ccttggcgat
    10981 ttgttgatga aagccaatta cggcggcctg gatgtcgaaa tcgcggcagt tattggtaac
    11041 cacgatactt tacgttctct ggttgagcgt tttgatattc cgtttgagct ggtaaaccat
    11101 gaagggttaa gccgcaacga gcacgatcaa aagatggcag acgccattga tgcttataaa
    11161 cctgactatg tggtgctggc gaagtatatg cgggtattaa cgccggaatt tgtggcacgc
    11221 ttcccgaata aaatcatcaa tattcaccat tcattcctgc ctgcgtttat cggcgcacgt
    11281 ccttatcacc aggcctatga acgtggtgtg aagattattg gcgcaaccgc tcactatgtg
    11341 aatgacaatc tggacgaagg cccaatcatc atgcaggacg ttattcatgt cgatcatacc
    11401 tacacagctg aagatatgat gcgcgcaggt cgtgacgtcg agaaaaacgt cttaagtcgt
    11461 gcgctataca aagtactggc acagcgcgtc tttgtttacg gtaatcggac gattattctt
    11521 taatcgccag caaaaataac tggttacctt taatccgtta cggatgaaaa ttacgcaacc
    11581 agttcatttt tctcaacgta acactttaca gcggcgcgtc atttgatatg atgcgccccg
    11641 cttcccgata agggagcagg ccagtaaaaa gcattacccc gtggtggggt tcccgagcgg
    11701 ccaaagggag cagactgtaa atctgccgtc atcgacttcg aaggttcgaa tccttccccc
    11761 accaccatct caagcattac ctcaaaattt gaattacccc tggtggggtt cccgagcggc
    11821 caaagggagc agactgtaaa tctgccgtca tcgacttcga aggttcgaat ccttccccca
    11881 ccaccatcac ttttcaaaag tccgtaaact ctcaaacgaa tccgcaatta aatattctgc
    11941 ccaagcgggg aaggatgaga agcttcgacc aaggttcgac ttgagcgcca gcgagagagc
    12001 gttgccgcag gcaacgaccc gaagggcgaa gcgcgcagcg ctgagtaatc cttcccccac
    12061 caccatcgct tccttgaata atcaaaacct gttactccga gctaattttg cccgatggca
    12121 ccacgtttac cgggcctaca atatcctccc ctgtaggtcg gataagcgaa gcgcatccga
    12181 caactcaccc cgcaccgtgc ccataaaaaa accccgccga agcagggtct atgtgaacta
    12241 aaattcgttt agtgacgagc gcgcaccagc tggtacttac gtgtcagata ctccaccggt
    12301 acgctccaga tgtgtaccag acgcgagaac gggaacagca ggaataacgt catccccagc
    12361 accaggtgca gacggaagat aaacgccaca ccatcgaggt gttgagaagc gccaccgtgg
    12421 aaggtcacca ccgactgcgc ccagccaacc agtttcatca tctcgctacc gtccatatgc
    12481 tgagcggaga acggaatggt caacaggccc agcgcgcact ggataacgag cagcgacagg
    12541 atcaggatat ccgctccggt agtggttgca cgcacgcgtg ggctgaacag acgacgcttc
    12601 agcaacagca cgccgccaat cagacacagc acgccgctgg caccaccagc aaacatcgcc
    12661 attttctgtt tcacttcaat cggcagccag gcttcataca tccagtgcgg cgtcagcata
    12721 ccgaagaagt gaccaacaaa aatccccaga ataccgatat ggaacaggtt cgacgccagg
    12781 ttcatccctt tgcgatccag catctggctg gacgccgcgc gccaggtgta ctgcccgtag
    12841 tcataacgca gccagctacc aatcaggaag accgccccgg ctatgtacgg gtagatatca
    12901 aagaagaaca tattcaggaa ttgcattagt gctgtcctcc ggtggtgata ttcagatatt
    12961 gcggcgcgac ggcaccggca aagcgacgct gatgagcagt gattgctgaa tcaccgcagc
    13021 ctttgtcagc aaagaattta acctgctctt cttcccagac agcatccagc gcctgcggcg
    13081 tatcatcgcg cgcttcgtcg gcaatttttt ccgccacttt gtcgctgtcg atagcggtat
    13141 tggccaattt cagcagcaga tcaaacatca cggcataacg gctttcacgc tgttgcagac
    13201 gcgcgctcag caatgccaga atcggcgcga tatctttcaa accttccacg gcttcgcttt
    13261 gcggcagctg cgacaggtac tccagataca gcggcagatg gtccggtaat tcgcggctgt
    13321 ttaactgcaa gccgtgctgc tcgtactgcg ccagcaggtc caccatcgcc tggccgcggt
    13381 cacgggattc accgtgaaca tgttcaaata gcaacagtga cgtggcgcgg ccacggtcga
    13441 acagttcgct gtactgggcc tgggcatcga gcggatccat cgccgttaaa tcgcgcagga
    13501 aaatgcccag cgcatgggca tcttctttcg acagattttt cgacgcagca atcgcctcaa
    13561 acatctcttg ttgatgctgc cataaggcag catccggata ttcaaggaga cgcgatacaa
    13621 tcacgagttc gatcatggat gcggctccgt tttgctggtc acatcgatgg catcgatacg
    13681 acggctgttg aacagattga atttggtatc tgagccgtgg cagccatcac caaaggtaaa
    13741 gccgcagcca tttttctccg ggaaggcttc ccgtgccagt tcacgatgac tactcggcac
    13801 cacaaagcga tcttcgtagt tagcaatcgc cagataacgg tacatctcct gtgcctgggc
    13861 ttcggtcaga ccgacctctt ccagcgcacg ggtatcaact ttaccgtcaa cggtttcagc
    13921 acgtttgtaa tgacgcatcg ccagcatacg tttcagtgcg cgcagtaccg gtttggtatc
    13981 accggcggtc agcagattcg ccagatactg taccggaata cgcaagcttt cgacgtctgg
    14041 cagaatgccg ttgctaccca gctcacccgc gtctgctgca gactgaatcg gagacagagg
    14101 cggcacgtac cagaccatcg gcagtgtgcg atattctgga tgcagcggca gcgccagttt
    14161 ccattccatt gccattttat aaaccggcga ctgctgtgcg gcttcaataa cgctcagcgg
    14221 aataccgtct ttaatcgcct gctcgatgac tttcggatcg ttcggatcga ggaacacctc
    14281 cagctgacgc tggtaaagat ctttctcgtt ctcggtgctg gctgcacgtt caatcgcgtc
    14341 ggcatcgtac agcagcacgc caagataacg gatacgaccg acacaggttt ctgagcacac
    14401 ggtcggctga cccgcttcaa tacgcggata gcagaagatg cacttctcag acttaccgct
    14461 cttccagttg aagtagattt ttttgtacgg gcatccggtg atgcacatac gccagccacg
    14521 gcatttatct tggtcgatca ggacgatgcc atcttcttca cgcttgtaaa tcgcaccgct
    14581 cgggcaggtc gccacacatg ccgggttcag gcagtgttcg cacaggcgcg gcaaatacat
    14641 cataaaggtg ttttcgaact ggctatacat cgccttctgg atgttgtcga agttcttgtc
    14701 tttcgccagt ttgtcaaact caccacccag gtcatcttcc cagttcggcc ctttttcgat
    14761 tttcgccatc cgttccccgg taatcagcga acggggacgg gcaatcggct gcgatttgct
    14821 gccttccggc gcggtatgca ggttctgata gtcaaaatcg aacggctcgt aataatcgtc
    14881 gatccccggc agatgcgggt tagcgaagat tttacccagc aacatggcac ggttacccat
    14941 gcgcggctgc agtttgccgt tgattttacg gatccagccg cctttgtatt tttcctggtt
    15001 ttcccagtca gtcgggaagc cctgacccgg cttggtttcc acgttgttga accacgcgta
    15061 ctccacgcct tcacggctgg tccagacgtt tttacaggta actgaacagg tgtggcagcc
    15121 gatgcactta tcgagattca gcaccatgcc gacttgtgaa cgaattttca ttttacgctc
    15181 tcctgtacct agtcattgcc ttcgccatct aaccagtcaa tgttcttcat cttacgcact
    15241 acaacaaact catcgcggtt agaacctacg gtgccgtagt agttaaagcc gtatgccaga
    15301 tgggcatagc caccaatcat atgcgtcggt ttcggcgtga tacgggtgac cgagttgtgg
    15361 atccccccac gctggccagt aatttccgaa cccggcaggt taacgatacg ttcctgcgcg
    15421 tggtacatca tggtcatccc tgccggaaca cgctggctga caaccgcacg ggcagtcagc
    15481 gcaccgttgc tgttgtaaac ttcaatccag tcgttatcgg caatgcccag ctctttggca
    15541 tcggcttcgc tcagccagac caccgggcca ccgcgaccta aggtcagcat caactggttg
    15601 tcgctgtagg tggagtggat cccccacttc tggtgcggcg tcaggaagtt gagcgctttt
    15661 tccgggttgc cgttggattt ctggcctatc acttctttca ccgaacgggt gtcgatcggc
    15721 ggacgataaa ccagcaggct ttcaccgaaa tcacgcatcc actggtgatc ctgatacagt
    15781 tgctgacgac cagagagcgt acgccatggg atcagctcgt gaacgttggt gtaaccggcg
    15841 ttgtaagaaa cgtgttcatc ttccagacca gaccaagtcg ggctggagat aattttgcgc
    15901 ggctgtgcct gaatatcgcg gaagcggatc ttctcgtctt ctttattcag cgccagatgc
    15961 gtatggtcac gaccggtaaa ttcgctcagg gcagcccagg ctttcacggc tacctgaccg
    16021 ttggtttccg gtgccagtgt caggatcatc tctgccgcat caattgcggt gttcagcatc
    16081 ggctggcctt tcgccggacc ttccgctttg gtgtagttga gcttacgcag cagatccatc
    16141 tcgctctggg tgttccaggc aatcccttta ccgccattac cgattttctc catcagcggg
    16201 ccgatagagg taaagcgttc gtaagtcgcc ggataagcgc gctctacgac cataatgtgt
    16261 ggcgcggttt tacctgggat caggtcgcac tcgccttttt tccagtcttt cacatccagc
    16321 ggctgcgcca gttcagcggc agagtcatgc tggataggca gcgtgacgat gtcggtttct
    16381 ttacccagat ggccgacgca cacttcggag aatttcttcg cgatggcttt gtagatttcc
    16441 cagtcgcttt tcgcttccca ggccggatcg accgccgcag acagcgggtg aataaacgga
    16501 tgcatatccg aagtattcat gtcgtctttt tcgtaccagg tcgccgtcgg caaaatgatg
    16561 tcggaataga gacaggtgct cgacagacgg aagtccagcg taaccaccag atccagcttg
    16621 ccttccagac cattgtcctg ccagtccact tcttccggct tcacgccgcc ctgttgcccc
    16681 agatctttac cctggatacc gtgctccgtc cccagcaggt acttgagcat aaactcatga
    16741 cctttaccgg aagaaccgag caggttagaa cgccagatga acaggttgcg cgggtggttt
    16801 ttaccgtttt ctggttgttc tgccgcaaaa cggatggaac cctctttcag ggatttcacc
    16861 gtatagtcca ccggattcat cccggctttt tccgcttcgc cagcgatagt cagcgggtta
    16921 gtgcctaact gcggtgcaga cggcagccag cccatgcgtt ccgcacggac gttaaagtcg
    16981 atcaagtgtc cggtatagcg ggatttgtcc gccatcggtg acagcaactc ttccgccgtg
    17041 acggtttcat aacgccactg gctggagtgg ttatagaaat aagaagtgct gttcatgtga
    17101 cgcgccggac gctgccagtc aagggcaaac gccagcggct gccagccggt ttgcggacgc
    17161 agtttttcct gacctacata gtgcgcccag ccgcccccgc tctgaccgac acagccgcag
    17221 aaaatcagca tgttgatcag accacgatag ttcatatcga ggtgatacca gtggttcagc
    17281 cccgcaccaa cgataatcat cgaacgaccg tgcgttttat cagcgttatc ggcaaattca
    17341 cgggcgatgc gaataatctg gctgcgagaa acgccggtaa tctgctcggc ccaggccggg
    17401 gtataagctt tcacatcgtc atagctggtt gcacagttaa cgtcgttcag gccacgttcc
    17461 agaccgtagt ttgccagcgt cagatcataa acggtggtca ccagggcggt gctgccatca
    17521 gccagttgca ggcgtttcac cggcagtttg tgcagcagca cgttttccag ttccactttg
    17581 ttgaagtgtt ccgtgccgtc gccaccaaag tacgggaagc ctacctcagc gatctcatct
    17641 tggctaccca gcaggctcag ttgcagctcg gtttcttcgc cagttttgcc gtcgcgctgc
    17701 tcaagattcc atttgccctt ctcgccccag cggaagccaa tagaaccgtt cggcgcaacc
    17761 atttcgccat tggtattaaa ggcgacagtt ttccattccg gattgttttc ctggcccagc
    17821 gcatcaacca gatcagcagc gcgcagcata cgacctgcag cgtagtaacc gtcgcgttct
    17881 tccagcatca ccagcatcgg catgtcggtg tagcgacgca catagtcggt gaaatactgg
    17941 cttgggttgt cgaggtggaa ttcacgcagc attacgtggc ccatcgccag cgccattgcc
    18001 gcatcggtgc cctgtttcgg tgccagccac agatcgcaca gtttggcgat ttcagcgtag
    18061 tctggtgtga cggcaacagt tttggtgcct ttgtaacgca cttcagtaaa gaagtgagca
    18121 tccggggtac gcgtctgcgg cacgtttgaa ccccaggcga tgatgtagct ggagttatac
    18181 cagtcagcag attccggtac gtcagtttgc tcgccccagg tttgcggaga cgcaggaggc
    18241 aggtcgcagt accagtcgta gaagcttaag caagtaccgc caatcagcga gagatagcgt
    18301 gcacccgatg cgtaagaaac catcgacatt gccggaattg gcgagaaacc agcaacacgg
    18361 tccgggccgt agtttttgat ggtgtaaacg ttagatgcgg cgatcagttc gttcacctcc
    18421 tgccaggaag aacgaacaaa tccaccgcgt ccacgcgcct gcttaaagct tttcgcttta
    18481 tcggcgtctt caatgataga agcccatgcc tcaaccggat cgctatgcag cgccttcgct
    18541 tcacgccaca ttttcatcag gcgtttgcgc atcatcgggt atttcaggcg gttggcactg
    18601 taaagatacc aggagtagct ggcaccgcgc gggcagccgc gaggttcatg gtttggcaga
    18661 tccggacggg tacgcggata gtcagtctgc tgggtttccc aggtgaccag accgtttttg
    18721 acgtagattt tccagctgca ggagccggtg cagtttaccc cgtgggtaga gcggacgatt
    18781 ttgtcatgct gccaacgctg gcgatatcca tcctcccagt cacggttggt attgagaagc
    18841 tggccatgcc catcggcaaa ggtttcatcc ttctgtttga agtagcgaaa ccggtccagg
    18901 aatttactca tcggttttct cctgtgggag cctgtcggcc ctctgataaa tcgacattgc
    18961 taatgtttaa gggcaaggta acgctctgaa acagcgtggg aattgataac gatcaagagt
    19021 gatggggata aaaaataaag taaattccta agatacccct ttaggataat taacagccac
    19081 cacataacag actgtaaatt aagacgtttt atacctgtag ttatttaaac tattaagatt
    19141 tttatgcaga tgggtattaa ggagtattcc ccatgggtaa catattaatg gctcttaatg
    19201 aaatccggcg aaataaacca tataacatta gtatgttgta actaaatagc gcttaaaaaa
    19261 agcgcgacat catgccgcgc aaaggataat caaatatttt actctatata attcggttta
    19321 caggaaggct gcaagtgaat gaatcccagg agcttactaa agtaagtgac tggggtgaat
    19381 gaacgcagcc gcagcacatg taacccgaag tatgacgagt aacggtttat tttttagaat
    19441 gccgaccata taccgcccag gtaatcacta cgcaggcgat atagaaaatc aaaaatacct
    19501 tcattgcgcc gactggcgaa cccgttaatg ccagcgagct accaaacgct ttcgggataa
    19561 agaagccacc aatcgcgcca atcgcagaga tgaaacccag cgccgccgcc gtgtcggttg
    19621 ccgcttcacg catcgcacgt tcgtcagacc ccccttctgc tttcacgcga tccattgtca
    19681 gtttacggaa gatcactgaa atcatctgga aagtggaacc actacccagc ccagctgtca
    19741 ggaacagcgc caggaagacc gcgaagaacg ccatgaagct tccgccctgc ccgtcagtcg
    19801 gtaaggtcag gaacagcagg ccgctgaaaa tcgccatcag aataaagttc accagcgtga
    19861 cacgagttcc gcccagacgg tcagataatg caccacctgc agaacgcgcc agcgcaccaa
    19921 taaacggccc gaagaaagcg tattgcagaa tctgaacatc cgggaactgc gtttttgaca
    19981 gcattgcaaa gcccgcggag aagccgatga aggagccgaa ggttgccaga tacagcaggc
    20041 tcataatcca cagatgaccc cgtttgagta ccggcaactg ctccttgatg gaggctttcg
    20101 aggtagcaag atcgttcatg ccaaaccacg ccgcaatggt gaagatggca aggaacggca
    20161 cccatatcca ggacgcattc gccagataca gctcagtccc atccggctgt ttgacgccct
    20221 ggctaccaaa tactgcgaaa atcgacagtg ataccaccag cggagcaacc aactgcatga
    20281 cgctgacgcc catgtttccc agaccaccat tcagacccag cgcgccaccc tgcttctgtt
    20341 tcggaaagaa gaagctgatg tttgccatac tggatgcgaa gttcgcgcca gcaaagccgc
    20401 atagcagaga gatgatgatg aagacgctat aaggcgtgga ggtatcctgc acggcaaaac
    20461 ccagccagac gcaaggaata atcagaatac cggtgctgaa cgccgtccag cgacgaccac
    20521 cgaagatagg aaccataaag gagtatggaa cacgtaataa cgcgccagaa accgaaggca
    20581 gcgcagtcaa cataaatagc tgatcggtcg taaaattaaa accgactttc ggtaagttca
    20641 ccgcaacagc gctgaacaac atccatacgc aaaacgccag cagcagacag ggaacggaaa
    20701 tccacaggtt acggctggca atacgttgac cgcgttgttg ccagaacgca ggatcttccg
    20761 gtcgccaatc tgtaatgaca gctccagtag ccctttcggg ggcggatgag tgactcatag
    20821 acacctctga tactcgtttc gatgtctgcc accttagtgt ctgtagctaa aggcaatttg
    20881 atgtaaatca aacgataaga taactttatc tatcattgat atttatcatt acccatagtg
    20941 agtacagtga tttcataaaa attatgagat ttttcacggt gctgtaaaat ccctaccctt
    21001 accgatgtaa agcgactaac cacacggcaa ataaggagta actctttccg ggtatgggta
    21061 tacttcagcc aatagccgag aatactgcca ttccagaatg tatcgtcaca ttcattaagg
    21121 ttattgctca tttaaagcct gaaggaagag gtttacatgc ttaaacgttg tctctctccg
    21181 ctcaccctgg ttaatcaggt tgcgcttatt gtgttgcttt ctactgctat tggactggca
    21241 gggatggcgg tttctggctg gctggtgcaa ggcgttcagg gcagcgccca tgcgatcaac
    21301 aaagcgggat cgctgcgcat gcaaagttac cgtctgttgg cggcagtgcc attaagtgag
    21361 aaagacaagc ccttaattaa agagatggaa caaacggcat ttagcgccga gttaactcga
    21421 gcagcagaac gagacgaaca actggcgcaa ttacagggtt tacaagatta ctggcgtaat
    21481 gaactgatcc ctgcactgat gcgtgcacaa aaccgcgaaa cggtgtcagc ggatgtcagc
    21541 cagtttgttg ccgggcttga tcagctggta tctggttttg accgcaccac ggaaatgcgc
    21601 attgagacag tggtactggt ccatcgggta atggcggtat ttatggcact tttactggtg
    21661 ttcactatta tctggttgcg ggcgcgactg ctacaaccgt ggcggcaact gctggcaatg
    21721 gcgagtgccg tcagtcatcg cgattttacc caacgcgcga acatcagcgg gcgcaacgaa
    21781 atggcgatgc ttggaactgc attgaacaat atgtctgcag aactggccga aagttatgcc
    21841 gtacttgagc agcgggttca ggagaaaacc gccgggctgg agcataaaaa tcagatcctc
    21901 tcttttttat ggcaggctaa ccgccgtttg cattcccgcg ccccgctgtg tgaacgcctg
    21961 tcacctgtac tcaacggctt acagaattta accctgctac gtgatatcga actgcgggtg
    22021 tatgacactg atgatgaaga gaatcatcag gagtttacct gccagccaga tatgacttgt
    22081 gatgataaag gctgccagct ctgcccgcgc ggcgtattac ccgttggtga tcgcggcaca
    22141 accctgaagt ggcggctggc tgactctcat acgcagtacg gtattttgct ggcgaccctg
    22201 ccgcaggggc gtcatcttag ccatgatcaa caacaactgg tggataccct ggttgaacaa
    22261 ctcaccgcca cgctggcgct ggatcgccat caggaacgtc agcaacagtt gatcgtgatg
    22321 gaagagcgtg ccaccattgc gcgcgaactg catgattcta ttgcccaatc tctctcttgc
    22381 atgaagatgc aggtgagttg tttacagatg cagggcgatg cgctgccagc aagcagccgc
    22441 gaactgttaa gtcagatccg taacgaactg aatgcatcct gggcgcagtt gcgtgaattg
    22501 ctcaccacat tccgcttgca gctcaccgag cctggattac gtccggcgct ggaggcgagt
    22561 tgcgaagagt acagcgccaa atttggcttc ccggtgaagc tggattatca attgccgcct
    22621 cgtctggtgc cttcacatca ggcaatccac ttgttgcaaa ttgcccgtga ggcattaagt
    22681 aacgccctca aacattcgca agcgagtgag gtcgtggtga cggtggcgca aaacgataat
    22741 caggtcaaac tgaccgtcca agataacggc tgcggcgtgc ctgaaaatgc catccgcagc
    22801 aatcactacg gcatgataat aatgcgcgac cgtgcgcaaa gtttacgagg cgattgccgc
    22861 gtccgccgtc gtgaatcagg tggcaccgaa gtggtggtca cctttattcc cgaaaaaact
    22921 ttcacagacg tccaaggaga tacccatgag taatcaggaa ccggctacta tcctgctgat
    22981 cgacgatcac ccgatgctgc gaactggcgt aaaacagctt atcagtatgg caccagatat
    23041 caccgtggtt ggcgaagcga gtaatggcga acagggtatt gaactggcgg agtctcttga
    23101 tcccgatctg atcctgttag atctcaatat gcccggcatg aacggtctgg aaacgctgga
    23161 taaactgcgc gaaaagtccc tctcagggcg cattgtggta ttcagcgtct ctaaccatga
    23221 agaagatgtg gtcaccgcac tgaaacgcgg cgcggatggt tatctgttaa aagatatgga
    23281 accggaagat ctgctgaaag cattgcatca ggcagctgct ggcgaaatgg tattaagcga
    23341 agcattaacg cctgttctgg ccgccagctt gcgcgctaac cgtgccacta ctgagcgcga
    23401 tgttaaccag ttaaccccac gcgagcgcga tattctcaag ctgattgccc agggtttgcc
    23461 gaacaagatg attgcccgcc gcctggatat caccgaaagc acagtaaaag tgcacgtcaa
    23521 gcacatgctg aagaaaatga agctcaagtc tcgcgtggaa gcagcggtat gggtgcatca
    23581 ggagcgcatt ttctgattac ggttcccagc gcagttcgtc gtttgacaat gcatcgaacg
    23641 gttcgacaag cgttagcgtg atctcattgg aggagacacg ctgcccctgg ttatcttccg
    23701 ccaccaccga caatcgccag tgattgcttg ccccttcccc gttctgccag tcaggcatga
    23761 tcagcgtcca gccctccgcg ctgttggctt gtgcgcccgg cgtcaaactt aatatctgcg
    23821 tatcgccctg ccaaatcagt tgccgaatac cgtaacgact gcggatttgt aatttcagcg
    23881 gcactgtttc gccagatttt agatcccacg gcggtgtcgc cagaaacacc gttaacgttt
    23941 ttcgctgtcg gtactcaaga gtcggtagat tatttcgctg cggattgtca tagcgactac
    24001 cacgtaacga ctgactttcg gcaacctcgc ccgcagaaag ttgttttttg agcggtacac
    24061 caaagcggta attaagattc cgcccgaggt tattttgatt ctcgccactt tcaccctgtt
    24121 tatgctgggc cgtcacagtg actaatggca caggggtgta atttaatccc agactcaacg
    24181 cgacgggatt gtgataaccc gtaccagagt taaacaaatc aacacgatca ccaaaatact
    24241 gttctaggct gacactggta ttgaggtgtt gatagaacgg catgcgcatc cgagctgtca
    24301 ggtcgtaccc gcgcgccatc cgttgttcct gcgtggctgt ctgttcatgc catgcagcaa
    24361 acggctgata aaagtttgcc gataatcgca aatattcgcc ccacgcttcg gcaccaaagc
    24421 ccgctcgctg aagattttcg tccagcaagt tgtcataaaa agtgttataa cccaccagcc
    24481 agttgccgcg cgcccagcgt tgcccaacgc ccacattgct caccaaccca ttatcctgct
    24541 gagtaagacc aagctggctc caggtgagat aacgatcatt atcttgtaac ggcacaaacc
    24601 agcttccacg actgccggtg aaatgtcctt cgttatccac tttgacgtca acactggcat
    24661 ttccccacgg tgatagccag gactctacgt gctgattaac ctgttgacta agcgcgtcgc
    24721 ggacttttcc caatgcgaaa gcttttgcct gctcgccagt atccagcccg ttatcattca
    24781 tactggtttc gccgaaatct ttcacaattt cagcaaagtg tttttcccca tcatgatttt
    24841 cgggagccat gcctaaatcc ggcagaccat cattgttatt atcaaaggga tttgccgctt
    24901 tttgctcgaa ggtagattgt gcgttagctg taccgcctgc tacaagcaag agtaaataaa
    24961 acgggataat gcgaggaacg aaacggctca aaacgtcggt aacttccggt aataatgaaa
    25021 atcatagcct taaagaataa cgattactgg caacttttgt cgtattttct cgcgctttca
    25081 ggaaaatcct taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga
    25141 tgagtgtcta attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa
    25201 tagcgcacct tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg
    25261 agagcaggag agcaatctgg atctgcgtct gttcctcatg tctgatgcgg tcacagccgg
    25321 gttgcgcggg caaaaaccag gggaaggcta caacattcag caaatgctgg agatccttac
    25381 cgctcagaat gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagtac
    25441 acttcctctg attgatgggg tggaaatcgg tactcttgtg gaactggcgc aatggacgct
    25501 gtcagccgat aaagtgctca cattttaatg tttgaggggg cgtctgacgc cccactatgc
    25561 gggaagttta ctcaggcgaa tcccggccgt aacacaccat ccggataatt ctccgccagc
    25621 agttttttta ccgataccag caaatcattc agcccgtcgt cctgcattcc cagtttgata
    25681 agctcctgtt ccagtgaaaa caggtattgt gcgttggttc ccagcggacc gctcgccgcc
    25741 gcaatcaacg gcgcgatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg
    25801 tccataataa acacaatggc gtttactgtg catccatcat caagatcaag ctgacaccag
    25861 gttggcagat aacagccggt aatcatctcg cgcttccaca acagggttag ttcctgctcc
    25921 agcgtctctt ctggcagtcg ataggcgacg cctgtagtgc gtccgccctc tttcagtgca
    25981 agcatccgtc caggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg
    26041 cgatgccatc caaccagtgt accggtgcac gactcggtaa actccagtgc cggattccac
    26101 atcaacgatc cataaccgaa gatccacacc ggtccctcat caggtcgaca agccagcgtc
    26161 gccgccagcg aagccgctcg ttgctctgct gaccataaga gtgattcttc aatggcacca
    26221 aatgccgttt tacaatcggc attcatcaag aaatcacgcg ttatcacctt acacctccgc
    26281 cactgcatac ttccctgcga caactttttg gtgtcttccg gacattttta ctgctcattt
    26341 tgaaaccact aacgggacaa tatgcaatcc acggaccact tgcaaggcaa caccgcacga
    26401 atagctttaa cta
//
LOCUS       DAJTNA010000041        25315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTNA010000041 DAJTNA010000000
VERSION     DAJTNA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25315
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.042"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(235..705)
                     /locus_tag="OO161_001423"
     CDS             complement(235..705)
                     /locus_tag="OO161_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5462552.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLALAESTS"
     gene            complement(821..1372)
                     /gene="maa"
                     /locus_tag="OO161_001424"
     CDS             complement(821..1372)
                     /gene="maa"
                     /locus_tag="OO161_001424"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR5462553.1"
                     /translation="MSTEKEKMIAGELYRSADETLSCDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1544..1762)
                     /gene="hha"
                     /locus_tag="OO161_001425"
     CDS             complement(1544..1762)
                     /gene="hha"
                     /locus_tag="OO161_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5462554.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1788..2162)
                     /gene="tomB"
                     /locus_tag="OO161_001426"
     CDS             complement(1788..2162)
                     /gene="tomB"
                     /locus_tag="OO161_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5462555.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2708..5857)
                     /locus_tag="OO161_001427"
     CDS             complement(2708..5857)
                     /locus_tag="OO161_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5462556.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5880..7073)
                     /gene="acrA"
                     /locus_tag="OO161_001428"
     CDS             complement(5880..7073)
                     /gene="acrA"
                     /locus_tag="OO161_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5462557.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            7215..7862
                     /gene="acrR"
                     /locus_tag="OO161_001429"
     CDS             7215..7862
                     /gene="acrR"
                     /locus_tag="OO161_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5462558.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7990..11352
                     /gene="mscK"
                     /locus_tag="OO161_001430"
     CDS             7990..11352
                     /gene="mscK"
                     /locus_tag="OO161_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5462559.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLEQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11564..11725)
                     /gene="rsmS"
                     /locus_tag="OO161_001431"
     CDS             complement(11564..11725)
                     /gene="rsmS"
                     /locus_tag="OO161_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5462560.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11739..12266)
                     /gene="priC"
                     /locus_tag="OO161_001432"
     CDS             complement(11739..12266)
                     /gene="priC"
                     /locus_tag="OO161_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5462561.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            12336..12722
                     /locus_tag="OO161_001433"
                     /pseudo
     CDS             12336..12722
                     /locus_tag="OO161_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
     gene            12875..13426
                     /gene="apt"
                     /locus_tag="OO161_001434"
     CDS             12875..13426
                     /gene="apt"
                     /locus_tag="OO161_001434"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5462562.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13555..15486
                     /gene="dnaX"
                     /locus_tag="OO161_001435"
     CDS             13555..15486
                     /gene="dnaX"
                     /locus_tag="OO161_001435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5462563.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    14819..14883
                     /gene="dnaX"
                     /locus_tag="OO161_001435"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15539..15868
                     /locus_tag="OO161_001436"
     CDS             15539..15868
                     /locus_tag="OO161_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5462564.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15868..16473
                     /gene="recR"
                     /locus_tag="OO161_001437"
     CDS             15868..16473
                     /gene="recR"
                     /locus_tag="OO161_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5462565.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16583..18457
                     /gene="htpG"
                     /locus_tag="OO161_001438"
     CDS             16583..18457
                     /gene="htpG"
                     /locus_tag="OO161_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5462566.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18638..19282
                     /gene="adk"
                     /locus_tag="OO161_001439"
     CDS             18638..19282
                     /gene="adk"
                     /locus_tag="OO161_001439"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5462567.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            19414..20376
                     /gene="hemH"
                     /locus_tag="OO161_001440"
     CDS             19414..20376
                     /gene="hemH"
                     /locus_tag="OO161_001440"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5462568.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVMPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(20373..21332)
                     /gene="aes"
                     /locus_tag="OO161_001441"
     CDS             complement(20373..21332)
                     /gene="aes"
                     /locus_tag="OO161_001441"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR5462569.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPRLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            21484..22788
                     /gene="gsk"
                     /locus_tag="OO161_001442"
     CDS             21484..22788
                     /gene="gsk"
                     /locus_tag="OO161_001442"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5462570.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22921..24597)
                     /locus_tag="OO161_001443"
     CDS             complement(22921..24597)
                     /locus_tag="OO161_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5462571.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(24835..>25315)
                     /gene="fsr"
                     /locus_tag="OO161_001444"
     CDS             complement(24835..>25315)
                     /gene="fsr"
                     /locus_tag="OO161_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5462572.1"
                     /translation="SYYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIG
                     RKYVIWGSILGVEPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIG
                     MVSGLFFGFAFGMGGLGAAVLGLIADHTRIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         6335 a         6580 c         6509 g         5891 t
ORIGIN      
        1 gggccagtct acagcaatac tgaaagaatc agtactcccg aaataaggcc gactagatgt
       61 cgtgttctca ccagaatatc cttattaaca cagtgtaggg aaagtatatc tgattgtgga
      121 aaaaggctgg caggagataa ggaggtagag ctggtatttc atgaagtgat gggtggaaac
      181 gatcattgtc atttgaccaa gcgcagagct ttacgcctgg tatgtaaccc taacttaaga
      241 tgttgattcg gcgagagcga gggtaaaaat atcgtaaaaa gacagttcac ctttacggac
      301 gatcattttt tgcagttgtt ccttatgaac atccgcgaca cgcggagagt tcaagatggc
      361 ttcaaccagc gcggcaggta caaagcgcgt gttgtaccat tcgccgttat agcaaacgcg
      421 gaaatccagc acgtcgatat cttcatagcg gtagcgtggg tagacatcga aaaagaagaa
      481 accattaagc aggataaata atacaaccag tagccagaca gagcctgaca gcgtttcgga
      541 ctgcaacatc accgccaggg tggcaaaaaa agcaacgtac ataccgataa acagccccgg
      601 atgtttacgg ataaaactga tactaaagcg gggtttgttg tcgcgttttt cgcgggtatt
      661 cagagactca atcgtttcgg tcagcaggcg ttgtatttcg gtcatcgttt gccttcgtgg
      721 tatctgcaac tttccaggga acaccctatt ttaacgtggg tatagtagag gggaagtaac
      781 agatctacca caattttgca taacagttgc gaaaaaccga ttacaatttt ttaattattc
      841 tggctggatt accgcccacg acaacgttgt ccgggacacc ttttgtgaca actgcacctg
      901 aagctaccac gacgttatca ccaatggtca caccagggtt aatgaccgcg cgtccgccaa
      961 tccagacgtt attaccgatg gtgacgggtt tccccagttc agcaccgcta ttacgtgcta
     1021 cagggtcgat ggggtgtgtt gccgtgtaga tatgaacgcc tggtgccaac atacagttat
     1081 caccgatgcg aatagggcag acatcaagca tcacgcaatc gaagttggcg aaaaaattat
     1141 tgccgagaaa aatgttatag ccataatcac agcgaaacgt tggctcaata taagcctctg
     1201 tcacctgacc gaataaatca gcgagaattt gctggcgtaa tgtgtgctct tccgccaggg
     1261 aatgattgta tcggtgaata agctgacgag cgcgcaggcg atcgcaagat aacgtctcat
     1321 ctgccgagcg atacaactca ccagcgatca tcttttcttt ttctgtgctc attactgacc
     1381 tccggttgct gaaaatgcgg caacacagta agacgaaaag tcagtgaaca gaacgttccc
     1441 attacatctg tgtgataaag atcacatagg gtatgtcttc atggtgaaaa agtataaaat
     1501 tcttaataaa cagccggtta tagctccgaa agcgaattat ttattagcga ataaatttcc
     1561 atactgagga agggatcttg tcgtatagtt tattcatggt caattcggcg aggcggtgat
     1621 ctgcggctga gtaaaatacc gccagttcat tatctgataa ttcgtattta tttttctcga
     1681 taacacgctc cagcgtgtca attgtctggc aacgacgtaa acgcattaaa taatcggttt
     1741 tcgttaaagg tttttcggac atacttctac ccatggttgt aataatacta acaagataaa
     1801 ctcgcaggat tctctttcgt cgcattgaca aaacaacgga atagtcgatt acctgacttc
     1861 cgccatttct gaagatcctg catattaata ccataactac tgaacaacat aaaggtgtca
     1921 tccagatatt cgtcgatctg ctcaatgagc ttattgtctt cattatactt aattttgtaa
     1981 ttaagtgcga aggtcgcaat atgctcaatc agttcattca actggaggtt gatcgccgag
     2041 gttgggtcgt taacccagcc ataattgctt tcttcaaggt ttgcaaggca gtcatgatac
     2101 agggtttcac agagaaactt aagctgtgcg atatcatgtc ttttgggtga gtattcatcc
     2161 ataacgcgtc cccttcttag cggttgaact aacggacacc tttcgggatg gaaaaaactt
     2221 actgacctgg acttgccctc tcgtttgtta gcttaactat aagccactct ttgcaggttt
     2281 tcatcgcatt attacgaaaa attacaatta ggaaggaatc gggacaggac tcactttttc
     2341 atactagcac taacccgcag caggtaaaag cagtttgctc gaactgttca taacatttca
     2401 ggattatcgc cagatgaaat tggtgacact aataccagga ttgctctgaa tatgacgtaa
     2461 taaccgagga atgaataaag aattaccgca aataattaag aatagcctct aaatgattat
     2521 ggattattaa atctattagc gctacttaat ataaattaac taacaatgag tattttttat
     2581 gaattttatc cgtggttaat actggttttc gtatgagatc ctgagttggt ggttcaatta
     2641 ctccttaatg ttcgtaggtt atgcataaaa aaggccgctt gcgcggcctt agtgattaca
     2701 cgttgtatca atgatgatcg acagtatggc tgtgctcgat atcttcattc ttgcggctaa
     2761 agcggcggcg aaccaccaca aagaataccg gaacgaagaa gattgccagt accgttgcgg
     2821 tcaccatccc gcccattaca ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag
     2881 tactgataac cagcggcata acgccgagga taaacgccag cgaggtcatc aggatcggac
     2941 gtaaacgcat ccgcactgca tcaagcgtcg cttcaatcag acctttacct tctttatcca
     3001 tcaagtcttt ggcgaattcg acgataagta tcgcgttctt cgccgacaac ccaatggttg
     3061 tgagcaggcc tacctggaag taaacgtcat tggtcaggcc acggaaggtg gcagccagca
     3121 acgcaccgat aacccccagc ggaacgacca gcataacgga gaacggaatc gaccagctct
     3181 cgtacagcgc cgccagacac aggaatacga caatcaacga aatcgcgtac agtgaaggtg
     3241 cctggttgcc ggagaggcgt tcctgatagg acatccccgt ccagtcatag ccaacaccgg
     3301 taggcagttt gctcgccagt tgttccatta gctccattgc ttcaccggta cttttacccg
     3361 gtgccgcctg gcctaagatt tccatggatg gcaggccatt gtaacgttcc agacgcggcg
     3421 aaccgtactc ccaacgagaa gaggagaatg ccgagaacgg caccatctga ccatcagcag
     3481 cacgaacata ccagtcgccg atatcatccg gcagcatacg gtatttcgct tctgacatga
     3541 cgtaaacttt cttcacacga ccgcggtcga taaagtcgtt cacatagcta ccgccccatg
     3601 cagcgcccag agtggtgtta atgtcgttga tagaaacacc cagcgcctgc gctttttcct
     3661 ggtcgatatc aatcttaaac tgcggggtat cttccagacc gtttggacgt acgctggtca
     3721 acatatcagg atgctttgct gcttctgcaa gcagctggtt acgcgcctga gtcagttttt
     3781 cgtgaccaag gccagcctgg tcaatcagct caaagtcaaa gccggttgca gtacccagtt
     3841 ccacgattgc gggcaggtta aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc
     3901 gtgttgcacg catggtaatc gcttcaactt tgttttcttc gcccggacga tcggcccagt
     3961 ccttcaagga aacgaacgca ataccggtat tctgaccacg tcccgcaaag ccgaagccgt
     4021 taacggcgaa caccgactca acgttgttct tttctttggt cagatagtaa tgcgttacct
     4081 cattgagcac tttctgcgta cgttcctgcg ttgcacctgc tggcagctga accatggtca
     4141 taaatacgcc ctggtcctca tctggcaaga aggagcttgg cagacgcacg aacagatagg
     4201 ccatgccgac cacgatgatc agatacagca ccaggtaacg ccccgtactg cgcagaatac
     4261 cgcctacgct gtcggtgtag tggtgcgtgc tcttctcgaa catgcggtta aaccagccga
     4321 agaagccttt tttaccttcc ccgtgatcgc ctttggcaat cggtttcagc atggtggcac
     4381 aaagagccgg agtcaggatc aacgctacca gtaccgacag cgccattgct gaaacaatgg
     4441 taatagagaa ctgacgatag atagcaccag tagaaccgcc aaagaaggcc atcggtacga
     4501 ataccgctga cagtaccatc gcgataccga ccagagcgcc ctgaatctgc cccatcgact
     4561 tacgggtagc ttcttttggc ggcaaacctt cttccgccat aacacgctcc acgttttcta
     4621 ccacaacgat agcgtcatcc accaacaagc cgatggcgag caccatcccg aacattgtta
     4681 gcgtgtttat cgagaagcca aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg
     4741 gtacggcaat ggtcggaatc aacgtcgcgc ggaagttctg caggaacaga tacataacca
     4801 ggaacacgag gatgatcgct tcgaccagcg ttttaaccac ttcgtgaata gagattttca
     4861 cgaacggcgt ggtgtcgtac gggtaaacaa ttttcagacc cgacgggaag aacggttcca
     4921 tcttcgccag ttcagcacgg attgccgcag cggtatccag cgcatttgca ccggtcgcca
     4981 gcttgatccc cagaccggaa gccggttggc cgttaaactc tgcgatgatg tcgtagttct
     5041 caccacccag ctcaatcttc gccacatcac gcagcaggac gcgggaacca tcctgattca
     5101 ctttcagcag gattttgccg aactcttcag tagaggtcag acgcgtctga gcaataatag
     5161 aggcgttaag ctgttggcct ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct
     5221 gggcgttctg cgctttgatg gcggtaatga catcaaccgg cgttagctgg aatttgttca
     5281 gctcattcgg gttcatccag atacgcatcg cgtactgtga accgaacaac tgaacatcac
     5341 ccacgcccga cgtacggctg atggcatctt tcatattcgc cgctacgtag tcagagatat
     5401 cctcctgcgt catggtgcca tcggtgttga taacgccgac aaccatcagg aagctgctgg
     5461 atgatttctc aacgctcacc ccttgctgct gaacttcttg cggcagcaac ggcatcgcca
     5521 gctgcagttt gttctgcacc tgaacctgcg cgatatccgc atcagtacca gactcaaagg
     5581 tcagggtgat ctgcacggta cccgtggagt cactgttaga ggacatgtac atcaggttat
     5641 cgataccgtt catattctgt tcgataacct gtgtcaccgt gtcctgcact gttttcgcat
     5701 cagcgccggg gtaggaggcg gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg
     5761 ccaccggcag tttgaggatc gccagccccc ctgccaacat gatgataatg gcgatcaccc
     5821 acgcaaaaat cgggcgatcg ataaagaaat taggcatgtc ttaacggctc ctgtttaagt
     5881 taagacttgg actgttcagg ctgagcaccg cttgcggctt gctggttatt atcagcggta
     5941 acttcttgtg cttttacctg gacaccagga cgcactttct gcagcccact tattactacg
     6001 cgatcgcctg ctttcagacc ttctgtcacc agccacttat cgccaatagc ctggcttgca
     6061 acgatcggac gggtttccac tttgtcatcc gcgccaacta ccagtacggt ggcatcacca
     6121 cgcggcgtac gggttacgcc ctgttgcggg actaaaatag cgtttggatt aagcccttct
     6181 tccagacgtg cgcgcacgaa catacccggc agcagagtgt gatccgggtt cgggaagata
     6241 gcgcgtaggg tgatagaccc agtggtctga tcaacggtaa cgtcagagaa ttccagcgta
     6301 ccgtcctgcg ggaacttaat gccgtcactg gtgatcagcg acactttggc tttgccgttc
     6361 tcttgtttca gcgtgccatt cgccagttcc tgtttcaggc gcaggaagtc gttgctggac
     6421 tgggtcacat caacgtagat cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga
     6481 ccgttctgta ccaatgcgcc ttccgtcacg ttcgacttac caatgcgacc gctaatcgga
     6541 gcggtgactt tggtgtaagc cagattgatc cgcgcagttt caacggcagc tttcgccgca
     6601 gttaccgcag cattcgcctg ttgcgcatca gccagagcct gatcgtactc ttgcttactg
     6661 atgtactgag taccgagcag tttctgataa cgattcaccg tcaattgcgc gatattggct
     6721 gcagcctggg ctttcgccag atcacctttc gcactgtcgt atgtcgcctg ataggtcgca
     6781 ggatcaatct gatagagaga gacaccagct tcgatgtcgc taccttcttt gaaattacgc
     6841 ttcaggataa tcccgctaac ttgaggacga acttctgcga tccggtaggc actggtgcga
     6901 cccggaagct cggttgtgat ctgcagaggt tcagttttga ctgttactac gccaacggcg
     6961 ggcatctgct ggccaccttg ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg
     7021 cctgagagca tcagaacgac cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa
     7081 cctcgagtgt ccgatttcaa attggtcaat ggtcaaaagt taataaaccc attgctgcgt
     7141 ttatattatc gtcgtgctat ggtacataca ttcacaaatg tatgtaaatc taacgcctgt
     7201 aaattcacga acatatggca cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca
     7261 tcctcgatgt ggctctacgt cttttctcac agcagggggt atcatccacc tcgctgggtg
     7321 agattgcaaa agcagctggc gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt
     7381 cggatttgtt cagtgagatc tgggaactgt cagaatccaa tattggtgaa ctagagcttg
     7441 agtatcaggc aaaattccct ggcgatccac tctcagtatt aagagagata ttaattcatg
     7501 ttcttgaatc cacggtgaca gaagaacgac gtcgattatt gatggagatt atattccaca
     7561 aatgcgaatt tgtcggagaa atggctgttg tgcaacaggc acaacgtaat ctctgtctgg
     7621 aaagttatga ccgtatagaa caaacgttaa aacattgtat tgaagcgaaa atgttgcctg
     7681 cggatttaat gacgcgtcgg gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg
     7741 aaaactggct ctttgccccg caatcttttg atcttaaaaa agaagcccgc gattacgtcg
     7801 ccatcttact ggagatgtat ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat
     7861 aaccctgaat ctgactccag gatttttcct ggacattttc gtcgttgcta ttctggttca
     7921 ctgcgtcgtg atattcttgc ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct
     7981 tcattcatca tgactatgtt ccagtattac aaacgatcac ggcattttgt tttttcagca
     8041 tttattgctt ttgtttttgt cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg
     8101 aatggtgatc tgccgacaaa agcggacctg caggcgcaac ttgactcact aaataaacaa
     8161 aaagatcttt ctgctcagga caaactggag cagcaggatc tgacagatac attagccacc
     8221 ctcgataaaa tcgatcgcat aaaagaagag acagttcagc tacggcaaaa agtcgctgaa
     8281 gcgccggaaa aaatgcgcca ggcgaccgcg gcgttaacag cacttagcga tgtcgataac
     8341 gacgaagaaa cgcgcaaaat tctgagtacg ctttcgttgc gccagctgga aactcgcgtt
     8401 gcccaggcgc tggacgattt gcaaaacgca caaaacgatc tggcgtctta taacagccag
     8461 ctggtttcgt tacagacgca gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag
     8521 cagctgcaac aaattcgcag tcgtctggat gggactgatg tcggcgagac tgccttacgt
     8581 cccagccaga aagtgttaat gcaggctcag caggcgttgc tgaatgcgga gattgaccag
     8641 cagcgtaaaa gcctggaagg gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat
     8701 tacgtgacgg cgaacagcgc ccgtctggag caccagttgc agctgttgca agaagcggta
     8761 aacagcaagc gcctgacttt aactgaaaaa acggcgcagg aagccgtctc cccggatgaa
     8821 gccgcgcgta ttcaggctaa tccgctggtg aaacaggaac tggaaattaa ccagcagtta
     8881 agtcagcgtc tgattaccgc gactgaaaac ggtaatcagt tgatgcagca aaacattaaa
     8941 gtcaaaaact ggctggagcg ggcgctgcaa tcggaacgca atattaaaga gcagattgcc
     9001 gtcctgaagg gcagcctgct gttgtctcgt atcctttacc agcaacaaca aacgctgccc
     9061 tcggcggatg aactggaaaa catgaccaac cgcatcgcgg atttgcgtct cgaacagttt
     9121 gaagttaacc agcagcgtga tgcactcttc cagagcgatg cgttcgtcaa caaactggaa
     9181 gaaggtcaca ccaacgaagt caacagcgaa gttcacgatg cgttattgca agtggttgat
     9241 atgcgtcgcg aattgctgga tcaactcaac aaacagttgg gtaaccagct gatgatggcc
     9301 attaacctgc aaatcaacca gcagcagtta atgagcgtat cgaaaaacct gaaatccatc
     9361 ctgactcagc aaatcttttg ggtgaacagt aaccgtccaa tggactggga ctggatcaaa
     9421 gcgttcccgc aaagcctgaa agatgaattt aagtcgatga aaatcacggt gaactgggaa
     9481 aaagcctggc ctgccgtttt tatcgctttc ctcgccggtt tgccgctgct gttgattgcc
     9541 gggctgatcc actggcgtct gggctggctg aaagcgtatc aacaaaaact ggcttccgct
     9601 gtgggttccc tgcgtaacga tagccagctc aacacaccaa aagcgatcct tatcgacctg
     9661 atccgcgcgc tgccggtgtg cctgattatt ctcgcggttg gcctgattct gttgaccatg
     9721 cagctcaaca tcagcgaact gctatggtcg ttcagcaaaa aactggcgat tttctggctg
     9781 gtgtttggcc tgtgctggaa ggtactggag aagaacggcg ttgccgtgcg tcacttcggc
     9841 atgccggaac agcagaccag ccactggcgt cggcaaattg tccgcatcag tctcgcattg
     9901 ctgcctatcc atttctggtc tgtggtggca gaactttccc cgctgcatct gatggatgat
     9961 gtgctggggc aagcgatgat tttcttcaac ctgctgctga ttgccttcct ggtatggcca
    10021 atgtgccgcg aaagctggcg tgataaagag tcgcacacca tgcgactggt caccattacc
    10081 gtgctgtcga ttatcccgat tgcattgatg gtgctgactg ccacgggtta tttctacacc
    10141 acgctgcgcc tctccgggcg ttggattgaa accgtttatc tggtgatcat ctggaacttg
    10201 ctctaccaga cggtattgcg cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt
    10261 gcgctggcgc gtcggcagaa tctggtgaaa gagggcgcag aaggtgccga accgccggaa
    10321 gaacccacca ttgcactgga acaggttaac cagcagacgc tgcgtattac catgttgctg
    10381 atgtttgcgc tgttcggtgt catgttctgg gcaatttggt ccgatttgat caccgtgttc
    10441 agctatctcg acagcattac gctctggcat tacaacggta ctgaagctgg cgctgcggtg
    10501 gtgaaaaacg tcaccatggg cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg
    10561 gcgttgattc gcaacctgcc tggtttgctg gaagtgctgg tgctctcgcg actgaatatg
    10621 cgccagggcg cgtcgtatgc cattactacc atccttaact acatcattat tgccgttggt
    10681 gcgatgacgg tgttcggatc gctgggcgtc tcttgggata aactccagtg gctggcggca
    10741 gcattatccg taggtcttgg ttttggttta caagaaattt tcggtaactt cgtctccggt
    10801 ttgatcattc tgttcgaacg tccggtgcgt attggcgata cggtaaccat tggtagcttc
    10861 tcggggacgg taagtaagat ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa
    10921 gaagtgatca tcccgaacaa agcgtttgtt accgagcgtc tgatcaactg gtcgttgact
    10981 gacactacta cgcgtctggt gatccgtctc ggcgtggcct atggctccga tctggaaaaa
    11041 gtgcgtaaag tgttactgaa ggcggcgact gagcacccaa gggtgatgca cgaaccaatg
    11101 ccggaagtct tctttacggc gtttggtgcc agcacgttgg atcatgagct gcgtctgtat
    11161 gtgcgtgaac tgcgtgaccg tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag
    11221 ctgtgccgtg aaaacgacat caacattgcc tttaaccagc ttgaagtgca tctgcacaac
    11281 gagaagggcg atgaggtgac ggaagtaaaa cgcgactaca aaggcgatga cccgacgcca
    11341 gcggtagggt aataaccaaa gggggcgaca tttagttgcc ccgagattac tgacaaagtg
    11401 cgcgttgttc atgccggatg cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat
    11461 tcaatgtatt gcaggagcgg tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt
    11521 ttgcccacaa tcttagggga catttagcgg ccccatttat ttctcacttt tccgcctcat
    11581 catcgcgcgt taatttcttt tcataatcgc gttttacaat atccagcgcg cgcagcacgg
    11641 tgcgggctgg gatctgattt tcttccagca gcacaatcaa atcgacagcc agtttgacat
    11701 cgtcaggggc attttccagt gacatactct ctccattgct agcgggttaa gcgcgctagc
    11761 ctgttttcga ttttttccag cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc
    11821 acttcacgat gcagcgtttg ctgttccacg agatcggtca cccgcgccag gcgggctctg
    11881 cgttcggcaa ccatctcacg tagccgccgc tcaaaatcct gatgctgaat acgtttacgc
    11941 tgccagcggg caattttcgg tggcgcactg tcccactcgc gcaatgacca ggcggaggct
    12001 tcacgcgcga tggcttccag ttgtgccgcc agatgttccg ccagccaggc cacttgcggc
    12061 agctgttgct gctcaactgc atgacgaagc gcagccagat tatcgcccgc ctcatcgaga
    12121 caagcctgca gtgttgtcgc acgggtttga aaaaggtgtc tgtcaaaacg ggcacttagc
    12181 gtggcaaatt gcgctaccgg ggcacaacgc tgacgcagcg tagcgagctg tccttccagt
    12241 ttttccagca gcagggcggt tttcacgata agacctcaat gaaaatgata attgttatgc
    12301 taaagtagcc actctgtaag ctgacaacac tcaatatgca acgaatcatt ttaatcatca
    12361 ttggctggct ggcggtagtg ctgggtacgc tgggcgtggt attaccggta ttaccggtat
    12421 taccgacgac gccgtttatc ctgctggcgg cctggtgctt tgcccgttct tccccgcgct
    12481 ttcacgcctg gttgctgtac cgctgatggt ttggcagcta tctacgtttc tggcagaaac
    12541 atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg ctcacgtttg
    12601 ccatttctct gtggttcgtc cagatgccat gggtgcgcat catgttgctg gtaattctcg
    12661 cctgtttgct tttctatatg tggcgaattc cggtgattga tgaaaagcaa gaaaagcact
    12721 gaagcacaac aatcgcagtt gcaattattg cgtacagcca gtacattctg gcgttttcga
    12781 gcacaggcgc aggcggtcaa aggttaaaca actgttactt ttgatacgtt taaaacgcgc
    12841 cgtgagtacc accgtaacaa gcaggcatac acttatgacc gcgactgcac agcagcttga
    12901 gtatctcaaa aatagcatca aaagcattca ggactaccca aaacccggca ttcttttccg
    12961 cgatgtcacc agcttactgg aagacccgaa agcttacgct ctcagcatcg acttgctggt
    13021 tgagcgttac aaaaatgcgg gcattaccaa agttgtcggc accgaagcgc gtggcttctt
    13081 gtttggcgct ccggtagctc tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg
    13141 caaactgccg cgtgaaacca tcagtgaaac ttacgacctg gaatacggca ccgatcagct
    13201 ggagatccac gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct
    13261 ggcaaccggc ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt
    13321 ggctgacgct gcgttcatta tcaacctgtt cgatctcggc ggcgaacagc gtctcgaaaa
    13381 acagggcatt accagctaca gccttgtccc gttcccgggc cattaattat cgccagtctt
    13441 gtgctgccca cgctacggac agcacaagat gtgcattcag cctcgccgtt ctgacggggc
    13501 tgtgttagca ttaccccttc gtgaatccac cttcctgcgt ttcagagcct gccaatgagt
    13561 tatcaggtct tagcccgaaa atggcgccca caaacctttg ctgacgtcgt cggccaggaa
    13621 catgtgctga ccgcactggc gaacggcttg tcgttagggc gtattcatca tgcttatctt
    13681 ttttccggta cccgtggcgt cggaaaaacc tctatcgccc gactgctggc gaaggggcta
    13741 aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc
    13801 gagcaggggc gctttgtcga tctgattgaa atcgacgccg cctcgcgcac caaagttgaa
    13861 gatacccgcg acctgctgga taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt
    13921 tatctgatcg acgaagtgca tatgctgtcg cgccacagct ttaacgcact gttaaaaacc
    13981 cttgaagagc cgccggagca cgttaagttt ctgctggcga cgaccgatcc acagaaattg
    14041 ccggtgacga ttttgtcgcg ctgtttgcaa tttcatctca aggcgctgga tgtcgagcaa
    14101 attcgccatc aacttgagca catcctcaac gaagaacata tcgctcacga gccgcgggcg
    14161 ttgcaattgc tggcgcgcgc cgctgaaggc agcctgcgag atgccttaag tctgaccgac
    14221 caggcgattg ccagcggtga cggccaggtt tcaacccagg cagtcagtgc gatgctgggt
    14281 acgcttgatg acgatcaggc gctgtcgctg gttgaagcga tggtcgaggc caacggcgag
    14341 cgcgtaatgg cgctgattaa tgaagccgct gcccgtggta tcgagtggga agcgttgctg
    14401 gtggaaatgc tcggcctgtt gcatcgtatt gcgatggtac aactttcgcc tgctgcactt
    14461 ggcaacgaca tggccgccat tgagctgcgg atgcgtgaac tggcgcgcac cataccgccg
    14521 acggatattc agctttacta tcagacgctg ttgattggtc gcaaagaatt accgtatgcg
    14581 ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt ccatccacgt
    14641 atgcctctgc ctgagccaga agtgccgcgc cagtcctttg cacccgtcgc gccaacggca
    14701 gtaatgacgc caacccaggt gccgccgcaa ccgcaatcag cgccgcagca ggcaccgact
    14761 gtaccgctcc cggaaaccac cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg
    14821 cagggagcaa ccaaagcaaa aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg
    14881 aataacgctg cgctggaaag actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg
    14941 ccatcggcgc tggaaaaagc gccagccaaa aaagaagcgt atcgctggaa ggcgaccact
    15001 ccggtgatgc agcaaaaaga agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa
    15061 catgaaaaaa cgccggaact ggcggcgaag ctagcggcag aagccattga gcgcgacccg
    15121 tgggcggcac aggtgagtca actttcgcta ccaaaactgg tcgaacaggt ggccttaaat
    15181 gcctggaaag aggagagcga caacgcagta tgtctgcatt tgcgctcctc tcagcggcat
    15241 ttgaacaacc gcggtgcgca gcaaaaactg gctgaagcgt tgagcacgtt aaaaggttca
    15301 acggttgaac tgactatcgt tgaagatgat aatcccgcgg tgcgtacgcc gctggaatgg
    15361 cgtcaggcga tatacgaaga aaaacttgcg caggcgcgcg agtccattat tgcggataat
    15421 aatattcaga ccctgcgtcg gttcttcgat gcggagctgg atgaagaaag tatccgcccc
    15481 atttgatcgt aagcacagct tacgttcgtc atccttaacg tgattgagag agaaacctat
    15541 gtttggtaaa ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat
    15601 gcagaaaatg caggaagaga tcgcgcagct ggaagtcacc ggcgaatctg gcgcaggtct
    15661 ggtaaaagtg accatcaacg gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct
    15721 gctggaagac gacaaagaga tgctggaaga cctggtggct gcagcattca acgacgcagc
    15781 acgtcgtatt gaagaaacgc agaaagaaaa aatggcctct gtatcctccg gaatgcagct
    15841 gccgcctggc tttaagatgc cgttctgatg caaaccagcc cgctgttaac acagcttatg
    15901 gaagcactgc gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg
    15961 ctgcttcagc gcgatcgtag cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg
    16021 tcggaaatcg gccactgcgc cgattgccgc actttcaccg aacaggaagt ctgtaacatc
    16081 tgttcgaatc cgcgtcgtca ggaaaacggt caaatctgtg tggtggagag tccggcggac
    16141 atctacgcca ttgagcagac ggggcagttt tctggtcgtt attttgtgtt gatggggcat
    16201 ctgtcaccgc tggacggcat cggtccggat gatatcgggc ttgatcgtct ggaacagcgt
    16261 ctggcagagg aaaaaatcac tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa
    16321 gctaccgcta actacattgc cgagctttgc gcgcaatatg acgtggaagc cagccgaatc
    16381 gctcatggcg taccggttgg cggcgaactg gaaatggtcg atggcaccac gctgtcacac
    16441 tcccttgccg ggcgtcataa gattcgtttt taagcaaacg agagcaggat cacctgctct
    16501 cgcttgaaat tattctccct tgtccccatc tctccctcat cctgttttta accttaaaat
    16561 ggcattattg aggtagacct acatgaaagg acaagaaact cgtggttttc agtcagaagt
    16621 gaaacagctt ctgcacctga tgatccattc tctctattcc aataaagaaa tcttcctgcg
    16681 tgagcttatc tctaacgcct ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa
    16741 cccggacctg tacgaaggtg atggcgaact gcgcgttcgt gtctctttcg ataaagacaa
    16801 gcgcacgctg accatctccg ataacggcgt ggggatgacc cgcgacgaag tgattgacca
    16861 tctggggact atcgctaaat ccggtaccaa atcattcctc gaatccctgg gttctgacca
    16921 ggcgaaagac agccagctga tcggtcagtt tggtgttggt ttctactctg cgtttatcgt
    16981 ggccgacaaa gtgactgtgc gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt
    17041 ctgggaatcg gctggcgaag gtgaatatac cgttgccgac atcaccaaag aagatcgtgg
    17101 tactgaaatc accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt
    17161 gcgttccatc atcagcaaat actccgacca tatcgcgctg ccggtagaga tcgaaaaacg
    17221 cgaagagaaa gacggcgaaa ccgttatctc ctgggagaaa atcaacaaag cgcaggcgct
    17281 gtggactcgt aacaagtcgg aaatcaccga tgaagagtac aaagagttct acaaacacat
    17341 cgcccacgac tttaatgatc cgctgacctg gagccacaac cgcgttgaag gtaagcagga
    17401 gtacaccagc ctgctgtaca tcccgtccca ggctccgtgg gatatgtgga accgtgatca
    17461 taaacacggc ctgaaactgt acgtccagcg tgtgttcatc atggacgacg cagaacagtt
    17521 catgccgaac tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa
    17581 cgtttcccgt gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca atgcgctgac
    17641 caagcgtgtg ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaaa aataccagac
    17701 cttctggcaa cagtttggcc tggtactgaa agaaggtccg gcggaagatt tcgctaacca
    17761 ggaagcgatc gccaaactgc tgcgttttgc ttctacccat accgattctt ctgcgcagac
    17821 cgtatctctg gaagactacg tttcccgcat gaaagaaggg caggagaaaa tctactacat
    17881 caccgcagac agctatgcgg cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa
    17941 aggcatcgaa gttctgctgc tttccgaccg catcgatgag tggatgatga actatctgac
    18001 tgagttcgac ggtaaaccgt tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact
    18061 ggctgacgaa gttgatgaga gcgcgaaaga agcggagaaa gcactgactc cgttcatcga
    18121 ccgtgtgaaa gccttgctcg gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac
    18181 cgatacgcca gcgatcgttt cgaccgacgc ggacgaaatg agcactcaga tggcgaaact
    18241 gttcgctgcg gcgggccaga aagtgccaga agtgaaatac atcttcgaac tgaacccgga
    18301 tcacgtactg gtgaaacgtg cggcagatac tgaagatgaa gcgaagttca gcgagtgggt
    18361 agaactgctg ctggatcagg cgctgctggc agaacgcggc acgctggaag atccgaacct
    18421 gtttattcgt cgtatgaacc agctgctggt ttcctgatgt catgccggat gacctttgtg
    18481 tcatccggca tttttctttt catcatctgc actttccgca aattatctcg ccattaaccg
    18541 tttcagcctc aggtgccttt cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt
    18601 ttcaaaaaat tcgacacatt ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc
    18661 tccgggcgcg gggaaaggga ctcaggctca gttcatcatg gagaaatatg gtattccgca
    18721 aatctccact ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca
    18781 agcaaaagac attatggatg ctggcaaact ggtcaccgac gaactggtga tcgcgctggt
    18841 taaagagcgc attgctcagg aagactgccg taatggtttc ctgttggacg gcttcccgcg
    18901 taccattccg caggcagacg cgatgaaaga agcgggcatc aatgttgatt acgttctgga
    18961 attcgacgta ccggacgaac tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc
    19021 gtctggtcgt gtttatcacg ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt
    19081 taccggtgaa gaactgacta cccgtaaaga cgatcaggaa gaaaccgtac gtaaacgtct
    19141 ggttgaatac catcagatga ctgcaccgct gatcggctac tactccaaag aagcggaagc
    19201 gggtaacacc aaatacgcga aagttgacgg caccaagccg gttgctgaag ttcgcgctga
    19261 tctggaaaaa atcctcggct aatcaagccg cagcagatct gctttcgggc aggtctgctc
    19321 atccacctca ggcaatacct ctcacagcaa ttagttcttc ttcctcactt ttccgctaca
    19381 attatcaaca agttgaatcg ataagaggcg gtaatgcgtc agactaaaac cggtatcctg
    19441 ctggcaaacc tgggtacgcc cgatgccccc acacctgaag cggtaaaacg ctatctgaaa
    19501 caatttttaa gcgacagacg cgtggttgat acctcacggt tgttatggtg gccattgctg
    19561 cgcggcgtta ttttgccgct gcgctcgccg cgtgtggcga agctgtatgc ctctgtctgg
    19621 atggaaggtg gctcgccgct gatggtttac agccgccagc aacagcaggc gctggcacaa
    19681 cgtttaccgg agacgcccgt agcgctggga atgagctacg gctcgccatc actggaaagc
    19741 gccgtcgatg aactcctggc agagcatgta gatcatattg tggtgatgcc gctttatccg
    19801 caattctcct gttctacggt cggtgcggta tgggatgaac tggcacgcat tctggcgcgc
    19861 aaacgtagca ttccggggat atcgtttatt cgtgattacg ccgataacca cgattacatt
    19921 aatgcactgg cgaacagcgt acgcgcttct tttgccaaac atggcgaacc ggatctgctg
    19981 ctgctctctt atcatggcat tccccagcgt tatgcagatg aaggcgatga ttacccgcaa
    20041 cgttgccgca caacgactcg cgaactggct tccgcactgg ggatggcacc ggaaaaagtg
    20101 atgatgacct ttcagtcgcg ctttggccgg gaaccctggc tgatgcctta taccgacgaa
    20161 acgctgaaaa tgctcggaga aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt
    20221 gctgcggatt gtctggagac gctggaagag attgccgagc aaaaccgtga ggtcttcctc
    20281 ggtgccggcg ggaaaaaata tgaatatatt ccagcgctta atgccacgcc agaacatatc
    20341 gaaatgatgg ctaatcttgt tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg
    20401 ccgtcgcgaa gagcctcgtc ggcggttttc atcattcgtg aataatgcaa aaaggcatgc
    20461 agcgtacctg ggtagagttt gaactcacag ggctgctgat gcgccgctaa cgtctggtaa
    20521 agcagacggc tgtcatccag ccgcggatcg aactccgccc cggcaataaa acagggcgga
    20581 acttcgcgag tgagatcatt attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg
    20641 ttgcttaaat acgcctcttc atacatctgc aaatcctgtt gcgttaagcc atcccagaca
    20701 ccgcccaaca gacgacgagt cacggaatcc cgtaatccgt aaagcccata ccacagcaaa
    20761 acacccgcaa ttttaccgca atcgatctgt ttatcacgca accacaacgc actggcgagc
    20821 gccagcatgg cacctgcgga atcaccggca aagccaatgc gggacatatt gatttgataa
    20881 tcctccgcct gctggtggaa ataacaacaa gcagccacaa tttcctctat cgcttgcgga
    20941 aaacgcgctt caggtgaaag ggggtaatca ataccaatca ccgtacattg gctgtagctt
    21001 gccagcaggc gcatgatgcg atcgtgggta tcgagattgc cgagaataaa accgcctcca
    21061 tgcaaataaa atagcgtcgc cgggctatct ggctgcggac aaaagagacg tgtttccacc
    21121 tgcccatatt ttgttggaac catgtaagct ctggttgcca tttctggagc gcccgcattc
    21181 cagaatcggc gctcaagcgt gtaatactgt cgttgctcag caatcgttcc cgttgcgggc
    21241 caggacggta aatccggctg aagagtattc acaacggtct tcatttcagc agaaataagg
    21301 tccagaacag gtagtttgtt ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt
    21361 gtaaggaacc actgccatga aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc
    21421 tcgcgcgctc ccgtcagatt gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca
    21481 accatgaaat ttcccggtaa acgtaaatcc aaacattact tccctgtaaa tgcacgcgat
    21541 ccgctgcttc agcaattcca gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt
    21601 gaccagacgc tggtcgatat tgaagcgaaa gtggatgatg aattcattga gcgttatgga
    21661 ttaagcgccg ggcattcact ggtgattgag gatgacgtag ccgaagcgct ttatcaggag
    21721 ctaaaacaga aaaacctgat tacccatcag tttgcgggtg gcaccattgg taacaccatg
    21781 cacaactact cggtgctcgc ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat
    21841 attgaaattg gcagttatgc ctatcgttac ctgtgtaaca cttccagccg taccgatctt
    21901 aactatctac aaggcgtgga tggcccgatt ggtcgttgct ttacgctgat tggcgagtcc
    21961 ggggaacgta cctttgctat cagtccaggc cacatgaacc agctgcgggc tgaaagcatt
    22021 ccggaagatg tgattgccgg agcctcggca ctggttctca cctcttatct ggtgcgttgc
    22081 aaaccgggtg aacccatgcc ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat
    22141 aacgtaccgg tggtgctgac gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg
    22201 tggcagcaat tcctcaaaga tcacgtctct atccttgcga tgaacgaaga tgaagccgaa
    22261 gcgttgaccg gagaaagcga tccgttgttg gcatctgaca aggcgctgga ctgggtagat
    22321 ctggtgctgt gcaccgccgg gccaatcggc ttgtatatgg cgggctttac cgaagacgaa
    22381 gcgaaacgta aaacccagca tccgctgctg ccgggcgcta tagcggaatt caaccagtat
    22441 gagtttagcc gcgccatgcg ccacaaggat tgccagaatc cgctgcgtgt atattcgcac
    22501 attgcgccgt acatgggcgg gccggaaaaa atcatgaaca ctaatggagc gggggatggc
    22561 gcattggcag cgttgctgca tgacattacc gccaacagct accatcgtag caacgtacca
    22621 aactccagca aacataaatt cacctggtta acttattcat cgttagcgca ggtgtgtaaa
    22681 tatgctaacc gtgtgagcta tcaggtactg aaccagcatt cacctcgttt aacgcgcggc
    22741 ttgccggagc gtgaagacag cctggaagag tcttactggg atcgttaagt tatcgtcggt
    22801 tcgtaggcca gataaggcgt tcacgccgca tctggcattt ggctctcgat gcctgatgcg
    22861 acgctggcgc gtcttatcat gcctacatat ttttcatatt ttacatccgg caaccaccgt
    22921 ttaccccgtc accacctcac ccgccggtgg cgtttccagc agttccagca tggtacgggc
    22981 gatttcacgc tcgcccatca ctacctgatt cgcaccacgt tcggtgatat acgccacttc
    23041 atcgtcataa tgggcgcggg caataatctc aatatccgga tttttcgcgc gggcagatgc
    23101 cacaatctca cccgcttcat aaccgttggg aatcgtcagg atcagccatt ttgcacattc
    23161 cagatgcgcc agttgcataa tttcttcgtt cgccgcattg cccaatactg cgcggacccc
    23221 gcgctctcgc agctcatcaa cacgggttcg tgacgtctca atcaccacca gcggaatatc
    23281 agaggcgagc aatttctccc ccagcaggct gcctacacga ccgtaaccca ccagtagcgc
    23341 atggttgcaa atatccactg ggatctgctt ctcttcttcg attgcctctt ccagcgtctg
    23401 ctcttccagc gtttcggtct tcgccagata tttctccagt agtgcgaaca gtaccgggtt
    23461 gagcataatc gacaggatcg cccctgccag taccaggttt tgtccggcct gcggcagtaa
    23521 attcaatgcc attcccagtc ccgccaggat aaacgcgaac tcaccaatct gcgccaggct
    23581 ggcggcgatg gttaatgccg tacgttggga gtgaccaaac agtcgcacca ggaaaaatgc
    23641 ggctaacgac ttaccaaaca gaataatcgc cagcgtcgcc agcactgcca gcggttgctg
    23701 aatcagaatt aacggatcaa acaacatccc gacggagaca aaaaacagca ccgcaaacgc
    23761 gtcgcgcaat ggcagcgtat cgtgggcggc acggtgactc agttcagact cgttcagtac
    23821 catcccggca aagaacgcac cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa
    23881 ggcaatccct aacgccagcg ccagcaccga cagggtaaac agctcgcgag aaccggttgc
    23941 cgcgctgcgt gccataatcc acggcaccag acggcgacct accagcatca taatggcgat
    24001 aaatgcgatc actttgccga tggtgatccc catatcgact gcaagagtgg caaagcccac
    24061 atcgccctgt tccatcattc ctgccactgc gggcagcaac accagcgtca gaaccattac
    24121 caggtcttcc acaatcaacc aaccgatggc gatttgccca cgctgactgt caattaattg
    24181 ccgttcttca agtgcgcgca gtaacaccac ggtactggcg gtggaaagac ataaaccgaa
    24241 cacgataccg gtcattaacg accagcccag cacggcagag agcgccatac ccagcagcgt
    24301 cgccacggct atctgggcga tcgcaccggg aatggcgatg gcctttaccg ccatcaaatc
    24361 cttcagcgaa aagtgcaaac cgacgccaaa catcaacaga atgacgccca gttcagccag
    24421 ttccggcgca agcttggtat cggcaacaaa gcccggagtg aatggtcctg ccagcacacc
    24481 cgctaacaga tatcccacca gaggagaaat acgtagttta ttggccagca tgccgaggat
    24541 aaaggcgagc acaaggccgc caacaatggt ggtgataagc ggggtggcgt gatgcattcc
    24601 gtctcctttt cctggtggtt attgtccatt tttggccggg aaaaccaaaa ttacaggtaa
    24661 tagtttatga caatttcatt gatgatgttc atgaataatt attgaatttt gcagaaaaat
    24721 ggaatttgct gcaaaaaaag catggatcgg aaaacagaag gcggtttaac agaggaaagg
    24781 cttatggcga ctgtaaaacg atgcagccaa agtttgcctt tggctgcaat gaaatcagtc
    24841 tttatgccgg ttatcaggca ggaatatggt caacatcccc aatagtggca ggaaagcaca
    24901 gattttatag actaactcga ttctggtgtg atcggcgata agccccagaa ctgccgctcc
    24961 cagacctccc atgccaaaag caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc
    25021 cggaagtagc tcctgagcgt agaccagaat ggcagagaat gccgaagcga ggataaatcc
    25081 aataatcacc gttaaaaccc ccgtccagtg caggctggcg tagggtaaaa tcagcgtaaa
    25141 cggctcaacg ccgaggatag agccccaaat cacatatttc cgtccaattt tatcccctac
    25201 aggcccgccg atcaccgtac ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg
    25261 ggcattctgg atagataatc cgaatttttg catcagataa aaggtgtaat agctg
//
LOCUS       DAJTNA010000042        25133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTNA010000042 DAJTNA010000000
VERSION     DAJTNA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25133
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.043"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..404)
                     /locus_tag="OO161_001445"
     CDS             complement(<1..404)
                     /locus_tag="OO161_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5462573.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKKPAA"
     gene            783..1094
                     /gene="rpsJ"
                     /locus_tag="OO161_001446"
     CDS             783..1094
                     /gene="rpsJ"
                     /locus_tag="OO161_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5462574.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1127..1756
                     /gene="rplC"
                     /locus_tag="OO161_001447"
     CDS             1127..1756
                     /gene="rplC"
                     /locus_tag="OO161_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5462575.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1767..2372
                     /gene="rplD"
                     /locus_tag="OO161_001448"
     CDS             1767..2372
                     /gene="rplD"
                     /locus_tag="OO161_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5462576.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2369..2671
                     /gene="rplW"
                     /locus_tag="OO161_001449"
     CDS             2369..2671
                     /gene="rplW"
                     /locus_tag="OO161_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5462577.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2689..3510
                     /gene="rplB"
                     /locus_tag="OO161_001450"
     CDS             2689..3510
                     /gene="rplB"
                     /locus_tag="OO161_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5462578.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3527..3805
                     /gene="rpsS"
                     /locus_tag="OO161_001451"
     CDS             3527..3805
                     /gene="rpsS"
                     /locus_tag="OO161_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5462579.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3820..4152
                     /gene="rplV"
                     /locus_tag="OO161_001452"
     CDS             3820..4152
                     /gene="rplV"
                     /locus_tag="OO161_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5462580.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4170..4871
                     /gene="rpsC"
                     /locus_tag="OO161_001453"
     CDS             4170..4871
                     /gene="rpsC"
                     /locus_tag="OO161_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5462581.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4884..5294
                     /gene="rplP"
                     /locus_tag="OO161_001454"
     CDS             4884..5294
                     /gene="rplP"
                     /locus_tag="OO161_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5462582.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5294..5485
                     /gene="rpmC"
                     /locus_tag="OO161_001455"
     CDS             5294..5485
                     /gene="rpmC"
                     /locus_tag="OO161_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5462583.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5485..5739
                     /gene="rpsQ"
                     /locus_tag="OO161_001456"
     CDS             5485..5739
                     /gene="rpsQ"
                     /locus_tag="OO161_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5462584.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5904..6275
                     /gene="rplN"
                     /locus_tag="OO161_001457"
     CDS             5904..6275
                     /gene="rplN"
                     /locus_tag="OO161_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5462585.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6286..6600
                     /gene="rplX"
                     /locus_tag="OO161_001458"
     CDS             6286..6600
                     /gene="rplX"
                     /locus_tag="OO161_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5462586.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6615..7154
                     /gene="rplE"
                     /locus_tag="OO161_001459"
     CDS             6615..7154
                     /gene="rplE"
                     /locus_tag="OO161_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5462587.1"
                     /translation="MAKLHDYYKDEVVKKFMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7169..7474
                     /gene="rpsN"
                     /locus_tag="OO161_001460"
     CDS             7169..7474
                     /gene="rpsN"
                     /locus_tag="OO161_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5462588.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7508..7900
                     /gene="rpsH"
                     /locus_tag="OO161_001461"
     CDS             7508..7900
                     /gene="rpsH"
                     /locus_tag="OO161_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5462589.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7913..8446
                     /gene="rplF"
                     /locus_tag="OO161_001462"
     CDS             7913..8446
                     /gene="rplF"
                     /locus_tag="OO161_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5462590.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8456..8809
                     /gene="rplR"
                     /locus_tag="OO161_001463"
     CDS             8456..8809
                     /gene="rplR"
                     /locus_tag="OO161_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5462591.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8824..9327
                     /gene="rpsE"
                     /locus_tag="OO161_001464"
     CDS             8824..9327
                     /gene="rpsE"
                     /locus_tag="OO161_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5462592.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9331..9510
                     /gene="rpmD"
                     /locus_tag="OO161_001465"
     CDS             9331..9510
                     /gene="rpmD"
                     /locus_tag="OO161_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5462593.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9514..9948
                     /gene="rplO"
                     /locus_tag="OO161_001466"
     CDS             9514..9948
                     /gene="rplO"
                     /locus_tag="OO161_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5462594.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9956..11287
                     /gene="secY"
                     /locus_tag="OO161_001467"
     CDS             9956..11287
                     /gene="secY"
                     /locus_tag="OO161_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5462595.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11319..11435
                     /gene="rpmJ"
                     /locus_tag="OO161_001468"
     CDS             11319..11435
                     /gene="rpmJ"
                     /locus_tag="OO161_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5462596.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11582..11938
                     /gene="rpsM"
                     /locus_tag="OO161_001469"
     CDS             11582..11938
                     /gene="rpsM"
                     /locus_tag="OO161_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5462597.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11955..12344
                     /gene="rpsK"
                     /locus_tag="OO161_001470"
     CDS             11955..12344
                     /gene="rpsK"
                     /locus_tag="OO161_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5462598.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12378..12998
                     /gene="rpsD"
                     /locus_tag="OO161_001471"
     CDS             12378..12998
                     /gene="rpsD"
                     /locus_tag="OO161_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5462599.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRIVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13024..14013
                     /gene="rpoA"
                     /locus_tag="OO161_001472"
     CDS             13024..14013
                     /gene="rpoA"
                     /locus_tag="OO161_001472"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5462600.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14054..14437
                     /gene="rplQ"
                     /locus_tag="OO161_001473"
     CDS             14054..14437
                     /gene="rplQ"
                     /locus_tag="OO161_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5462601.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14544..14912
                     /locus_tag="OO161_001474"
     CDS             14544..14912
                     /locus_tag="OO161_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR5462602.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGKPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14923..15348
                     /gene="zntR"
                     /locus_tag="OO161_001475"
     CDS             14923..15348
                     /gene="zntR"
                     /locus_tag="OO161_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR5462603.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15404..15622
                     /gene="arfA"
                     /locus_tag="OO161_001476"
     CDS             15404..15622
                     /gene="arfA"
                     /locus_tag="OO161_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR5462604.1"
                     /translation="MSRYQHIKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15619..16029)
                     /gene="mscL"
                     /locus_tag="OO161_001477"
     CDS             complement(15619..16029)
                     /gene="mscL"
                     /locus_tag="OO161_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR5462605.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16159..17535)
                     /gene="trkA"
                     /locus_tag="OO161_001478"
     CDS             complement(16159..17535)
                     /gene="trkA"
                     /locus_tag="OO161_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5462606.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17557..18846)
                     /gene="rsmB"
                     /locus_tag="OO161_001479"
     CDS             complement(17557..18846)
                     /gene="rsmB"
                     /locus_tag="OO161_001479"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5462607.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18892..19839)
                     /gene="fmt"
                     /locus_tag="OO161_001480"
     CDS             complement(18892..19839)
                     /gene="fmt"
                     /locus_tag="OO161_001480"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5462608.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19854..20363)
                     /gene="def"
                     /locus_tag="OO161_001481"
     CDS             complement(19854..20363)
                     /gene="def"
                     /locus_tag="OO161_001481"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5462609.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20493..21617
                     /gene="dprA"
                     /locus_tag="OO161_001482"
     CDS             20493..21617
                     /gene="dprA"
                     /locus_tag="OO161_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5462610.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21589..22062
                     /locus_tag="OO161_001483"
     CDS             21589..22062
                     /locus_tag="OO161_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5462611.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22091..22633
                     /locus_tag="OO161_001484"
     CDS             22091..22633
                     /locus_tag="OO161_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR5462612.1"
                     /translation="MAKSALFTVRNNESCSKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22638..23210
                     /gene="tsaC"
                     /locus_tag="OO161_001485"
     CDS             22638..23210
                     /gene="tsaC"
                     /locus_tag="OO161_001485"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5462613.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23215..24033
                     /gene="aroE"
                     /locus_tag="OO161_001486"
     CDS             23215..24033
                     /gene="aroE"
                     /locus_tag="OO161_001486"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5462614.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLNCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24030..24287
                     /locus_tag="OO161_001487"
     CDS             24030..24287
                     /locus_tag="OO161_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5462615.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24263..24817)
                     /locus_tag="OO161_001488"
     CDS             complement(24263..24817)
                     /locus_tag="OO161_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5462616.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6424 a         5974 c         6545 g         6190 t
ORIGIN      
        1 aaggcagcgg gtttttttaa tgattctttt cctctcatga gcagaccaat aactacggct
       61 ccgcaagcga atgaggcagc aaggaaaacc agtcgtggta agaaggccca tgaatgccag
      121 gcgccaaggg cggcgagaaa cttcacatca ccatagccta atccttcttt atgtcgcagt
      181 atgcgatacc cccagtaaat gacagcgaat gtgccgtaac caacaattgt cccccataac
      241 gcatcggcta aaccatcagg atgacaaact tgatagaaca aaagacctga ccagagtaac
      301 gggcacgtaa atctgtcagg taacaagcca tgctttgcat cccagaaaaa aagtagggct
      361 gataaacaag cgtacaaaat cagaaaggga agggtagtgt ccataaaaca tcctctgtaa
      421 ttggagtatc accatcgaat aactccggat gctcagcaac agagagagcg ctgatttgtt
      481 aacctgattc cggtgaaaaa cgctatcttc acaaccttac aaatttcgtg gaatgcagct
      541 cgcaatctgc gcgttgcgat taatttctac ccgatgcttg cgtcaggctc agatttgcgt
      601 ataatgcgcg ggcttgtcgt agttgacagc aggttcaatc tgaacctcag ttagccgaat
      661 ttggctacct aacaatgctc ccaatcgggg agctacgtaa gaacggttac actctcccat
      721 caatcgtaat gggtctgagg agtaatcatt ttcgtttata aaataattgg agctctggtc
      781 tcatgcagaa ccaaagaatc cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc
      841 aagcaaccgc ggaaatcgtc gagactgcca agcgcactgg tgcgcaggtc cgtggtccga
      901 tcccgctgcc gacacgcaaa gagcgcttca ctgttctgat ctccccgcac gtcaacaaag
      961 acgcgcgcga tcagtacgaa atccgtactc acttgcgtct ggttgacatc gttgagccaa
     1021 ccgagaaaac cgttgatgct ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga
     1081 tcagcctggg ttaatcaggt cattgagcga ttgagaggtt gaaacaatga ttggtttagt
     1141 cggtaaaaaa gtgggtatga cccgtatctt cacagaagac ggcgtttcta tcccagtaac
     1201 cgtaatcgaa gttgaagcaa accgcgttac tcaggttaaa gacctggcta acgatggcta
     1261 ccgtgctatt caggtgacca ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc
     1321 tggccacttc gctaaagctg gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc
     1381 tgaaggcgaa gagttcactg taggtcagag cattagcgtt gaactgtttg ctgacgttaa
     1441 aaaagttgac gtaactggca cctctaaagg caaaggtttc gcaggtaccg ttaagcgctg
     1501 gaacttccgt acccaggacg ctactcacgg taactccttg tctcaccgcg ttccgggttc
     1561 tatcggtcag aaccagactc cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat
     1621 gggtaacgaa cgtgtaaccg ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa
     1681 cctgctgctg gttaaaggtg ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc
     1741 agctgtgaag gcgtaaggag atagcaatgg aattagtatt gaaagacgcg cagagcgcgc
     1801 tgactgtttc cgaaactacc ttcggtcgtg atttcaacga agcgctggtt caccaagttg
     1861 ttgttgctta tgcagctggt gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag
     1921 taactggttc cggtaaaaaa ccgtggcgcc agaaaggcac cggccgtgcg cgttctggtt
     1981 ctatcaagag cccgatctgg cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc
     2041 acagtcaaaa agttaacaag aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac
     2101 tggtacgtca ggatcgtctg atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta
     2161 agctgctggc acagaaactg aaagacatgg ctctggaaga tgtgctgatc atcaccggtg
     2221 agctggacga aaacctgttc ctggctgcgc gcaacctgca caaggttgac gtacgcgatg
     2281 caactggtat cgacccggtt agcctgatcg ccttcgacaa agtcgtaatg actgctgatg
     2341 ctgttaagca agttgaggag atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct
     2401 gcgtgcaccg cacgtttctg aaaaagcgtc tactgcgatg gaaaaatcca acaccatcgt
     2461 actcaaagtt gctaaagacg cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt
     2521 tgaagtcgaa gtcgaagtcg ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg
     2581 acagcgtatc ggtcgtcgta gcgactggaa aaaagcttac gtcaccctga aagaaggcca
     2641 gaatctggac ttcgttggcg gcgctgagta agtcggagga gtaacacaat ggcagttgtt
     2701 aaatgtaaac cgacatctcc gggtcgtcgc cacgtagtta aagtggttaa ccctgagctg
     2761 cacaagggca aaccttttgc tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac
     2821 aacaatggcc gtatcaccac tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt
     2881 gttgacttca aacgcaacaa agacggtatc ccggcagttg ttgaacgtct tgagtacgat
     2941 ccgaaccgtt ccgcgaacat cgcgctggtt ctgtacaaag acggtgaacg ccgttacatc
     3001 ctggccccta aaggcctgaa agctggcgac cagattcagt ctggcgttga tgctgcaatc
     3061 aaaccaggta acaccctgcc gatgcgcaac atcccggttg gttctactgt tcataacgta
     3121 gaaatgaaac caggtaaagg cggtcagctg gcacgttccg ctggtactta cgttcagatc
     3181 gttgctcgtg atggtgctta tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta
     3241 gaagcagact gccgtgcaac tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt
     3301 ctgggtaaag caggtgctgc acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg
     3361 atgaacccgg tagaccaccc acatggtggt ggtgaaggtc gtaactttgg taagcacccg
     3421 gtaactccgt ggggcgttca gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat
     3481 aaattcatcg tacgtcgccg tagcaaataa ttttagagga taagccatgc cacgttctct
     3541 caagaaaggt ccttttattg acctgcactt gctgaagaag gtagagaaag cggtggaaag
     3601 cggagacaag aagcccctgc gcacttggtc ccgtcgttca acgatctttc ctaacatgat
     3661 cggtttgacc atcgctgtcc ataatggtcg tcagcacgtt ccggtatttg taaccgacga
     3721 aatggtcggt cacaaactgg gtgaattcgc accgactcgt acttatcgcg gccacgctgc
     3781 tgataaaaaa gcgaagaaga aataaggtag gaggaagaga tggaaactat cgctaaacat
     3841 cgccatgctc gttcttctgc tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag
     3901 aaagtgtcgc aggctctgga tattttgacc tacaccaaca agaaagcggc tgtactggtc
     3961 aagaaagttc tggaatctgc cattgctaac gctgaacaca acgatggcgc tgacattgac
     4021 gatctgaaag ttacgaaaat tttcgtagac gaaggcccga gcatgaagcg cattatgccg
     4081 cgtgcaaaag gtcgtgcaga tcgcatcctg aagcgcacca gccacatcac tgtggttgtg
     4141 tccgatcgct gagactctgg agactagcaa tgggtcagaa agtacatcct aatggtattc
     4201 gcctgggtat tgtaaaacca tggaactcta cctggtttgc gaacaccaaa gaattcgctg
     4261 acaacctgga cagcgatttt aaagtacgtc agtacctgac taaggaactg gctaaagcgt
     4321 ccgtatctcg tatcgttatc gagcgtccgg ctaagagcat ccgtgtaacc attcacactg
     4381 ctcgcccggg tatcgttatc ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg
     4441 tagcggacat cgctggcgtt cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac
     4501 tggacgcaaa actggttgct gacagcatca cttctcagct ggaacgtcgc gttatgttcc
     4561 gtcgtgctat gaagcgtgct gtacagaacg caatgcgtct gggcgctaaa ggtattaaag
     4621 ttgaagttag cggccgtctg ggcggcgcgg aaatcgcacg taccgaatgg taccgcgaag
     4681 gtcgcgtacc gctgcacact ctgcgtgctg acatcgacta caacacctct gaagcgcaca
     4741 ccacttacgg tgtaatcggc gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta
     4801 tggctgctgt tgaacaaccg gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag
     4861 gccgtaaata aggagcgtcg ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc
     4921 acaaaggccg taaccgcggt ctggcgcagg gtacggatgt tagcttcggc agcttcggtc
     4981 tgaaagctgt tggccgtggt cgtctgactg cccgtcagat cgaagcagca cgtcgtgcta
     5041 tgacccgtgc agttaagcgt caaggtaaga tctggatccg tgtgttcccg gacaaaccga
     5101 tcactgaaaa gccgctggca gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg
     5161 ttgccttgat tcagccgggt aaagtcctgt atgaaatgga cggtgttccg gaagagctgg
     5221 cccgtgaagc attcaagctg gcagcagcga aactgccgat taaaaccacc tttgtaacta
     5281 agacggtgat gtaatgaaag caaaagagct gcgtgagaag agcgttgaag agctgaacac
     5341 cgagctgctg aacctgctgc gtgagcagtt caacctgcgt atgcaggctg caagtggcca
     5401 gctgcaacag tctcacctgt tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt
     5461 actgaacgag aaggcgggtg cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt
     5521 gttagcgaca aaatggagaa atccattgtt gttgctatcg aacgttttgt gaaacacccg
     5581 atctacggta aattcatcaa gcgtacgacc aaactgcacg tacatgacga gaacaacgaa
     5641 tgcggtatcg gtgacgtggt tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc
     5701 tggacgctgg ttcgcgttgt agagaaagcg gttctgtaat acagtacact ctctcgatac
     5761 gaataaacgg ctcagaaatg agccgtttat tttttctacc catatccttg aagcggtgtt
     5821 ataatgccgc gccctcgata tggggatttt taacgacctg attttcgggt ctcagtagta
     5881 gttgacatta gcggagcact aaaatgatcc aagaacagac tatgctgaac gtcgccgaca
     5941 actccggtgc acgtcgcgta atgtgtatca aggttctggg tggctcgcac cgtcgctacg
     6001 caggcgtagg cgacatcatc aagatcacca tcaaagaagc aattccgcgt ggtaaggtca
     6061 aaaaaggtga tgtgctgaag gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg
     6121 acggttctgt cattcgcttc gatggtaatg cttgtgttct tctgaacaac aacagcgagc
     6181 agcctatcgg tacgcgtatt tttgggccgg taactcgtga gcttcgtagt gagaagttca
     6241 tgaaaattat ctctctggca ccagaagtac tctaaggagc gaatcatggc agcgaaaatc
     6301 cgtcgtgatg acgaagttat cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt
     6361 aagaatgtcc tgtcttccgg caaggtcatt gttgaaggta tcaacctggt taagaaacat
     6421 cagaagccgg ttccggccct gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt
     6481 caggtttcca acgtagcaat cttcaatgcg gcaaccggca aggctgaccg tgtaggcttt
     6541 agattcgaag acggcaaaaa agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa
     6601 tttggagtag tacgatggcg aaactgcatg attactacaa agacgaagta gttaaaaaat
     6661 tcatgactga gtttaactac aattctgtca tgcaagtccc tcgggtcgag aagatcaccc
     6721 tgaacatggg tgttggtgaa gcgatcgctg acaaaaaact gctggataac gcagcagcag
     6781 acctggcagc aatctccggt caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag
     6841 gcttcaaaat ccgtcagggc tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca
     6901 tgtgggagtt ctttgagcgc ctgatcacta ttgctgtacc tcgtatccgt gacttccgtg
     6961 gcctgtccgc taagtctttc gacggtcgtg gtaactacag catgggtgtc cgtgagcaga
     7021 tcatcttccc agaaatcgac tacgataaag tcgaccgcgt tcgtggtttg gatattacca
     7081 ttaccactac tgcgaaatct gacgaagaag gccgcgctct gctggctgcc tttgacttcc
     7141 cgttccgcaa gtaaggtagg gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt
     7201 aaaacgcgta gctttagctg ataaatactt cgcgaaacgc gctgaactga aagcgatcat
     7261 ctctgatgtg aacgcttccg acgaagatcg ttggaacgct gttctcaagc tgcagactct
     7321 gccgcgtgat tccagcccgt ctcgtcagcg taaccgctgc cgtcaaacag gtcgtccgca
     7381 tggtttcctg cggaagttcg ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg
     7441 tgaaatcccg ggtctgaaaa aggctagctg gtaattgtca ccaattgaat cacgggagta
     7501 aagacagatg agcatgcaag atccgatcgc ggatatgctg acccgtatcc gtaacggtca
     7561 ggccgcgaac aaagctgcgg tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa
     7621 cgtgctgaag gaagaaggtt ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga
     7681 actggaactt actctgaagt atttccaggg caaagctgtt gtagaaagca ttcagcgtgt
     7741 cagccgccca ggtctgcgca tctataaacg taaagatgag ctgccgaaag ttatggcggg
     7801 tctgggtatc gcagttgttt ctacctctaa aggtgttatg actgatcgtg cagcgcgcca
     7861 ggctggtctt ggtggcgaaa ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg
     7921 tgttgctaaa gcaccggtcg ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt
     7981 tattacgatc aaaggtaaaa acggcgagct gactcgtact ctcaacgatg ctgttgaagt
     8041 taaacatgca gataataccc tgaccttcgg tccgcgtgat ggttacgcag acggttgggc
     8101 acaggctggt accgcgcgtg ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt
     8161 cactaagaag ctgcagctgg ttggtgtagg ttaccgtgca gcggttaaag gtaatgtgat
     8221 taacctgtct ctgggtttct ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc
     8281 tgaatgtccg actcagactg aaatcgtgct gaaaggcgct gataagcagg tgatcggcca
     8341 ggttgcagcg gatctgcgcg cctaccgtcg tcctgagcct tataaaggca agggtgttcg
     8401 ttacgccgac gaagtcgtgc gtaccaaaga ggctaagaag aagtaaggta acactatgga
     8461 taagaaatct gctcgtatcc gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg
     8521 cgcaactcgc ctggtggtac atcgtacccc gcgtcacatt tacgcacagg taattgcacc
     8581 gaacggttct gaagttctgg tagctgcttc tactgtagaa aaagctatcg ctgaacaact
     8641 gaagtacacc ggtaacaaag acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc
     8701 tctggaaaaa ggcatcaaag atgtatcctt tgaccgttcc gggttccaat atcatggtcg
     8761 tgtccaggca ctggcagatg ctgcccgtga agctggcctt cagttctaag gtagaggtgt
     8821 aagatggctc acatcgaaaa acaagctggc gaactgcagg aaaagctgat cgcggtaaac
     8881 cgcgtatcta aaaccgttaa aggtggtcgt attttctcct tcacagctct gactgtagtt
     8941 ggcgatggta acggtcgcgt tggttttggt tacggtaaag cgcgtgaagt tccagcagcg
     9001 atccagaaag cgatggaaaa agcccgtcgc aatatgatta acgtcgcgct gaataacggc
     9061 actctgcaac accctgttaa aggtgttcac acgggttctc gcgtattcat gcagccggct
     9121 tccgaaggta ccggtatcat cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg
     9181 gttcataacg ttctggctaa agcatatggt tccaccaacc cgatcaacgt ggttcgtgca
     9241 actattgatg gcctggaaaa tatgaattct ccagaaatgg tcgctgccaa gcgtggtaaa
     9301 tccgttgaag aaattctggg gaaataaacc atggcaaaga ctattaaaat tactcaaacc
     9361 cgcagtgcaa tcggtcgtct gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt
     9421 cgtattggtc acaccgtaga gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg
     9481 gtttccttca tggttaaagt tgaggagtaa gagatgcgtt taaatactct gtctccggcc
     9541 gaaggctcca aaaaggcggg taaacgcctg ggtcgtggta tcggttctgg cctcggtaaa
     9601 accggtggtc gtggtcacaa aggtcagaag tctcgttctg gcggtggcgt acgtcgcggt
     9661 ttcgagggtg gtcagatgcc tctgtaccgt cgtctgccga aattcggctt cacttctcgt
     9721 aaagcagcga ttacagccga agttcgtctg tctgacctgg ctaaagtaga aggcggtgtt
     9781 gtagacctga acacgctgaa agcggctaac attatcggta tccagatcga gttcgcgaaa
     9841 gtgatcctgg ctggcgaagt aacgactccg gtaactgttc gtggcctgcg tgttactaaa
     9901 ggcgctcgtg ctgctatcga agctgctggc ggtaaaatcg aggaataagt agcagatggc
     9961 taaacaaccg ggattagatt ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag
    10021 actgctgttt gttatcggtg cgctgattgt gttccgtatt ggctctttta ttccgatccc
    10081 tggtattgat gccgctgtac ttgccaaact gcttgagcaa cagcgaggca ccatcattga
    10141 gatgtttaac atgttctctg gtggtgctct cagccgtgct tctatctttg ctctggggat
    10201 catgccgtat atttcggcgt cgatcattat ccagctgctg acggtggttc acccaacgtt
    10261 ggcggaaatt aagaaagaag gggagtctgg tcgtcgtaag atcagccagt acacccgcta
    10321 cggtactctg gtgctggcaa tattccagtc gatcggtatt gctaccggtc tgccgaatat
    10381 gcctggtatg caaggcctgg tgattaaccc gggctttgca ttctacttca ccgctgttgt
    10441 aagtctggtc acaggaacga tgttcctgat gtggttgggc gaacagatca ctgaacgagg
    10501 tatcggcaac ggtatttcaa tcattatctt cgccggtatt gtcgcgggac tcccgccagc
    10561 cattgcccat actatcgagc aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt
    10621 ggttgcagta ttagtatttg cagtgacgtt ctttgttgta tttgttgagc gtggtcaacg
    10681 ccgcattgtg gtaaactacg cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag
    10741 cacacattta ccgctgaaag tgaatatggc gggggtaatc ccggcaatct tcgcttccag
    10801 tattattctg ttcccggcga ccatcgcgtc atggttcggg ggcggtactg gttggaactg
    10861 gctgacaaca atttcgctgt atttgcagcc tgggcaaccg ctttatgtgt tactctatgc
    10921 gtctgcaatc atcttcttct gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac
    10981 agcagataac ctgaagaagt ccggtgcatt tgtaccagga attcgtccgg gagagcaaac
    11041 ggcgaagtat atcgataaag taatgacccg cctgaccctg gttggtgcgc tgtatattac
    11101 ctttatctgc ctgatcccgg agttcatgcg tgatgcaatg aaagtaccgt tctacttcgg
    11161 tgggacctca ctgcttatcg ttgttgtcgt gattatggac tttatggctc aagtgcaaac
    11221 tctgatgatg tccagtcagt atgagtctgc attgaagaag gcgaacctga aaggctacgg
    11281 ccgataattg gtcgcccgag aagttacgga gagtaaaaat gaaagttcgt gcttccgtca
    11341 agaaattatg ccgtaactgc aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca
    11401 gtgccgagcc gaagcataaa cagcgccaag gctgattttt tcgcatattt ttcttgcaaa
    11461 gttgggttga gctggctaga ttagccagcc aatcttttgt atgtttgtgc gtttccattt
    11521 gagtatcctg aaaacgggct tttcagcatg gaacgtacat attaaatagt aggagtgcat
    11581 agtggcccgt atagcaggca ttaacattcc tgatcataag catgccgtaa tcgcattaac
    11641 ttcgatttat ggcgtcggca agacccgttc taaagccatc ctggctgcag cgggtatcgc
    11701 tgaagatgtt aagatcagtg agctgtctga aggacaaatc gacacgctgc gtgacgaagt
    11761 tgccaaattt gtcgttgaag gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct
    11821 gatggatctt ggttgctatc gcggtttgcg tcatcgtcgt ggtctcccgg ttcgcggtca
    11881 gcgtaccaag accaacgcac gtacccgtaa gggtccgcgc aaaccgatca agaaataatc
    11941 ggggtgattg aataatggca aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag
    12001 tctctgacgg cgtggctcat atccatgctt ctttcaacaa caccatcgtg actatcactg
    12061 atcgtcaggg taacgcgttg ggttgggcaa cagccggtgg ttccggtttc cgtggttctc
    12121 gcaaatccac tccgtttgca gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag
    12181 aatacggcat caagaatctg gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta
    12241 ctattcgtgc tctgaacgcc gcaggtttcc gcatcactaa cattactgat gtgactccga
    12301 tccctcataa cggttgtcgt ccgccgaaaa aacgtcgcgt ataacgcctc gttttccagg
    12361 tttgttggag aaagaaaatg gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg
    12421 agggcaccga cttattcctt aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg
    12481 aacaagctcc tggccagcac ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt
    12541 tgcgtgaaaa gcaaaaagtt cgccgtatct atggtgtgct ggagcgtcag ttccgtaact
    12601 actacaaaga agcagcacgt ctgaaaggca acaccggtga aaacctgttg gctctgctgg
    12661 aaggtcgtct ggacaacgtt gtataccgta tgggcttcgg tgccactcgt gcagaagcac
    12721 gtcagctggt tagccataaa gcaattatgg taaacggtcg tattgttaac atcgcttctt
    12781 atcaggttag tccgaatgac gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg
    12841 tgaaagccgc tctggagctg gctgagcagc gtgaaaagcc aacttggctg gaagttgatg
    12901 ctggcaagat ggaaggtacg tttaagcgta agccggagcg ttctgatctg tctgcggaca
    12961 ttaacgaaca cctgatcgtc gagctttact ccaagtaaag cttagtacca aagagaggac
    13021 acaatgcagg gttctgtgac agagtttcta aaaccgcgcc tggttgatat cgagcaagtg
    13081 agttcgacgc acgccaaggt gacccttgag cctttagagc gtggctttgg ccatactctg
    13141 ggtaacgcac tgcgccgtat tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt
    13201 gagattgatg gtgtactaca tgagtacagc accaaagaag gcgttcagga agatatcctg
    13261 gaaatcctgc tcaacctgaa agggctggcg gtgagagttc agggcaaaga tgaagttatt
    13321 cttaccttga ataaatctgg cattggccct gtgactgcag ccgatatcac ccacgacggt
    13381 gatgtcgaaa tcgtcaagcc gcagcacgtg atctgccacc tgaccgatga gaacgcgtct
    13441 attagcatgc gtatcaaagt tcagcgcggt cgtggttatg tgccggcttc tacccgaatt
    13501 cattcggaag aagatgagcg cccaatcggc cgtctgctgg tcgacgcatg ctacagccct
    13561 gtggagcgta ttgcctacaa tgttgaagca gcgcgtgtag aacagcgtac cgacctggac
    13621 aagctggtca tcgaaatgga aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt
    13681 gcggcaacca ttctggctga acaactggaa gctttcgttg acttacgtga tgtacgtcag
    13741 cctgaagtga aagaagagaa accagagttc gatccgatcc tgctgcgccc tgttgacgat
    13801 ctggaattga ctgtccgctc tgctaactgc cttaaagcag aagctatcca ctatatcggt
    13861 gatctggtac aacgtaccga ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct
    13921 cttactgaga ttaaagacgt gctggcttcc cgtggactgt ctctgggcat gcgcctggaa
    13981 aactggccac cggcaagcat cgctgacgag taaccggatc acaggttaag gttttactga
    14041 gaaggataag gtcatgcgcc atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca
    14101 tcgccaggct atgttccgca atatggcagg ttcactggtt cgtcatgaaa tcatcaagac
    14161 gactctgcct aaagcgaaag agctgcgccg cgtagttgag ccgctgatta ctcttgccaa
    14221 gactgatagc gttgctaatc gtcgtctggc attcgcccgt actcgtgata acgagatcgt
    14281 ggcaaaactg tttaacgaac tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg
    14341 tattctgaag tgtggcttcc gtgcaggcga caacgcgcct atggcttaca tcgagctggt
    14401 tgatcgttca gagaaagcag aagctgctgc agagtaatct gaagcaacgt aaaaaaaccc
    14461 gccccggcgg gtttttttat acccgtagca tccccactta tctacaatag ctgtactctt
    14521 tttgttcatc ccctggagta tttatgtggt tacttgacca gtgggcagag cgtcatatag
    14581 cagaagcgca agcgaaaggt gagtttgata acctggcagg tagcggcaaa ccattgatac
    14641 tggatgatga ttctcatgtg ccaccggaat tacgtgcggg gtatcgcttg ctgaagaatg
    14701 ccggttgctt accgccagaa cttgagcagc ggagggaagc aattcagctt ctggatatcc
    14761 tgagaggtat ccgtcacgat gatccgcaat atcaagaggt tagccgtcga ttgtcattac
    14821 tggaattgaa gctgcgacaa gctggattga gtaccgattt tttacgcggc gattatgctg
    14881 acaagttgtt ggacaaaatc aacgataact agtggagtat gtatgtatcg cattggtgag
    14941 ctggcaaaaa tggcggaagt aacacctgac acgattcgtt attacgaaaa acagcagatg
    15001 atggagcatg aagtgcgtac tgaaggcggg tttcgcctgt ataccgaaag cgatctccag
    15061 cgattgaaat ttatccggca tgccagacaa ctaggtttca gtctggagtc gatccgcgag
    15121 ttgctgtcga tccgcatcga tcctgaacac catacctgtc aggagtcaaa aggcattgtg
    15181 caggaaagat tgcaggaagt cgaagcacgg atagccgagt tgcagagtat gcagcgttcc
    15241 ttgcaacgcc ttaacgatgc ctgttgtggg accgcccata gcagtgttta ttgttcgatt
    15301 cttgaagctc ttgaacaagg ggcgagtggc gttaagagtg gctgttgatt ttttgcactg
    15361 gcaggattac actcgcgccg taaaataacc aattggagtt tttatgagcc gatatcagca
    15421 tattaaaggg cagataaggg ataatgcgat agaagcatta ctacatgatc ccttattccg
    15481 acagcgcgta gagaaaaata agaaggggaa aggcagttac atgcgaaaag gaaaacatgg
    15541 caatcggggg aactgggagg ccagtggcaa gaaagtaaat cactttttta ccactggtct
    15601 tctgctttca ggcgcttgtt aagagcggtt attctgctct ttcagcaaat cacgaatttc
    15661 tgccagtaat acttcttctt tagttggtgc aggtgcggca gctggttctt cttttttccg
    15721 attcagtttg ttgattagct taatcgccat aaagatggca aaggccacaa tcagaaaatc
    15781 aaagacgttt tgaatgaaga caccgtaatg catcacaaca gcagggatat ccccctgcgc
    15841 atcgcgtagc gtgacagcaa actgtttaaa gtcgatcccg ccaattaata agcccagagg
    15901 tggcatgatg atatcggcaa ccagtgaaga tacaatcttc ccgaatgccg caccgataat
    15961 gacacccacc gccaaatcca ccacgttccc gcgcatcgca aattcgcgaa attctttaat
    16021 aatgctcatg ttattctccc tatgaagccg acaaccataa gtctaacaaa tgttaagcca
    16081 ttttcctgcc aggaatgaat ttaattaatc cttgataatc aaagggaaat aataaggcgt
    16141 cattagacgc cttattaatt acaagaagaa agggcttggc tggaagagtc tttcgacgtc
    16201 ggtaataaac tttttatctg tgaggaacat aattacgtga tcgccttgct caatgcgcag
    16261 attgtcattg gcaatcatca cgtcgtttcc acgtaccacc gctccaataa tcgttcctgg
    16321 cggtagcttg atttcgtcaa tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg
    16381 agcaacggct tcaatagctt ctgctacgcc gcggcgcaat gaggaaacac caacaatatc
    16441 tgcttttcgc acatggctaa gcaacgcaga aatagttgct tgttgtggtg aaattgcaat
    16501 atcgatcacg ctcccctgaa ccagatccac ataagcgcga cgctggatca ataccatcac
    16561 ctttttcgca cccatacgtt tggcaagcat ggcggacatg atattggcct cgtcatcgtt
    16621 ggtgacggca ataaacagat caacttgatc gatatgttct tcggccagca gttcttgatc
    16681 cgacgcatca ccaaaaaaga cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg
    16741 ctgctgatta cgttcgatga gtttaacgct gtaatctttt tccagacgac gcgccagccc
    16801 tgcaccgata ttaccgccac caaccagcat gatccgctta tacggttttt ccagtcgctg
    16861 taattcactc atcaccgcgc ggatatgctg tgaagcggca ataaagaaca cttcatcacc
    16921 agcttcaaca atggtcgaac cttgtggacg aatggggcga tcgtggcgga aaattgctgc
    16981 cacacgagta tcgatatgtg gcatatgttc gcgcatggtc gaaagtgcat taccaatcag
    17041 cgggccgcca taataggctt taactacagc gaggctgact ttaccctcag cgaagttcac
    17101 cacctgcaat gcgcctggat actcaatcag tcggtaaata ttatcgataa ccaactgctc
    17161 tggtgcgatc agatgatcaa tcggtacagc atctgaatga aatagcttat cggcatcgcg
    17221 tacgtagtct ggtgagcgga tgcgagcgat gcgattaggg gtgttgaaaa gtgagtaggc
    17281 tacctggcag gcaaccatat tggtttcatc tgaactggtt acagcaacca gcatatcggc
    17341 gtcgtcggca cctgcctccc gcaatacgcg tggatgagag ccatgcccct gcacgactcg
    17401 caggtcaaat ttatcctgca aggtccgcag acgctcaccg ttggtatcga caacagtaat
    17461 atcgttgttc tcgccaacca ggttttccgc cagtgtgccg ccaacctggc cggcacccag
    17521 aatgataatt ttcatcagtc tcgacccgtt atctcatcac tttttgatta gcttagcgta
    17581 aaagaagccg tcgccctctt cggcaccagg cagattttgt ttacccggtt gctctggtgt
    17641 tcctgtttcg caaagttcgg catcagcggt acgttgcaaa aaagctttaa tctgcaggct
    17701 attctcttcc ggtaacaccg aacaggtggc atagaccagg gttccaccgg attttaaatg
    17761 cggccaaatg gcgtcgagaa tttcagactg caattgcgcg agttccggga tatcgcgatc
    17821 gcgacgtaac catttaatat ccggatggcg acgaatcacc cccgttgctg aacaaggcgc
    17881 atctaataaa atgcgatcaa actgttgctc gccacaccat tgggaagggt aacggccatc
    17941 accttgtttc acggttgcct tcataccaag gcgttttaaa ttgtcgtaaa cgcgagagag
    18001 gcgctgttcg tcaatatcaa ccgcaacaac ctgcgcttct ggtgccacct caaggatatg
    18061 cgttgtttta ccgccggggg cggcacaaag atccagaatg tgttcaccgt tttgtggcgc
    18121 aagccaggtc atgcaacctt gtgctgatgc atcctgaacg gtaacccatc cgtcttcaaa
    18181 accaggtagc gcatgaacag gtgcaggtgt ttccagacgt acagcatcag ggtaatccgc
    18241 atgcgggaaa cctttcattc ctgcttcatc cagcaatgca agccagctgt cgcgggaatg
    18301 atgcgtacga ttaacgcgca gccacattgg cggacgctgg ttattggctt cgacgatgga
    18361 ttgccactgc tctggatacg ctttttgcag acgcttcagc aaccaggaag gatgcagata
    18421 acgtgcatca ctggcattaa acttggctaa taactcttct tgctgacgct ggaactggcg
    18481 taatacaccg ttaatcaacc ctttaagttg cggacgctta attgcgatag cgccttcaac
    18541 cgtttcagcc agcgcagcat gaggtggaat gcgggtataa agcagttgat acaaaccaac
    18601 cataatcagg taatgcacag tgcgctgttt gccggtcatc ggacgggcca ttaacttatt
    18661 aatcagccag tctaactgcg aaagcgtacg cagtacgcca aagcacaact cttgaagaag
    18721 tgctttgtct ttatcagaaa ctttttgctg gagcggtggc agaatgttgc ttaatgattg
    18781 cccttgctcg acgacttgtt caacggcctg ggccgccatg ctacgtaaat tacgttgttt
    18841 tttcataagt ataaaaatgc ccggtaagac cgggcttaga agagtggact atcaggccag
    18901 acggttgccc ggaacaaacc attcccgacg agaattcagg aggtcttgcg cgctcatcgc
    18961 tttcttaccc gcaggttgta acgagagcag attcaggatt ccatcaccag ttgcaacctg
    19021 aatgccttgt ttgttggctt caaggatcgt tcctggtgca gcgttggttg ccgtatcaat
    19081 gaccgacgct ttccagactt taaccggctg tctttcaatt tccagccagc tcattggcca
    19141 tggattgaaa gcgcgaatgc agcgttcaag ctgtgctgcc gaaagtgacc agtcaatacg
    19201 cgcttcttct ttactcaact tttcggcgta agtgacaaga gtttcgtcct gaacttctgg
    19261 tttcgccgtg ccgtctgcca gttgtttcaa cgtggtgata agcccttgtg ggccaagctc
    19321 tgccagcttg tcgtacagcg taccactggt atcttctgca gtaatcgggc aggagagctt
    19381 atagagcatg tcaccggtgt ctaaaccgac atccatttgc ataatggtca caccagtttc
    19441 tgcatcaccc gcccatagtg agcgttggat tggtgcagca ccgcgccagc gtggcagcag
    19501 tgaaccatga acgttgatac agccaagacg cggcatctcc agcactgctt taggcagaat
    19561 taagccatag gcgacgacga ccataacatc agcctgcaga tcggcgacca gttgctggtt
    19621 ttcttgtgga cgcagggaaa caggttgaaa aacgggcaga cctttttcct cagccagaac
    19681 tttaaccggg ctgggcatca gttttttacc gcgtcctgcc ggtcggtctg gctgggtgaa
    19741 cacgccaacg acgttatgac cagaagacaa cagcgcgtcg agatgacgcg ctgcaaagtc
    19801 aggtgtaccc gcaaaaataa tacgtagtga ttctgacacg ttagttctta tccttaagcc
    19861 cgggctttca gacgatccag tttttcaact ttctgacgaa tacgttgttg tttcaacggt
    19921 gacagataat ccataaacag tttgccgacc aggtgatcca tctcatgctg aatacagatg
    19981 gctaacagac cgtctgcttc caattcaaat ggtttaccgt cgcggtccag ggcgcgaatt
    20041 ttaactttct ctgcgcgtgg cactaaagca cgttgttcag ggatcgacag gcaaccttct
    20101 tcaatgcctg tttcgccgct tttttctaaa agctccggat tgattaacac cagccgttcg
    20161 tcacggtttt ccgaaacatc aataacaatg atacgttgat ggatatcaac ctgggttgcc
    20221 gccaggccaa taccttcttc tgcgtacatc gtctcgaaca tatcatcgac gatacgctga
    20281 atttctgcat tcacttcttc taccggttta gcaactttgc gaagccgctc gtccggaata
    20341 tgtaacactt gcaaaactga cataaatctc cagagatgtg ttcaggagtt agaaagatta
    20401 tttcttctat tctagacaaa tccccctctg attgacagca tcactgacca atcgcaaaga
    20461 ttgctaaggc tgcttatggc agggagataa ggatggtcga tacagatatt tggctgcgtt
    20521 taatgagcat cagcagcttg tacggcgatg atatggtccg tatagctcac tggctggcaa
    20581 aacagtcgca tattgatgcg gttgtattgc agcaaacagg gcttacattg cggcaggcac
    20641 aacgctttct ttcatttccg cgaaagagta tcgaaagctc actttgttgg ttggagcaac
    20701 ccaaccatca tttaatccct gcggacagcg aattttatcc tcctcaactt ctggcgacga
    20761 cagattaccc cggcgcactg tttgttgaag gagaactgca cgcgctgcat tcatttcagc
    20821 ttgccgtagt ggggagtcgg gcgcattcat ggtatggcga gcgatgggga cgattatttt
    20881 gcgaaactct ggccacgcgt ggagtgacaa ttacgagtgg actggcgcgt ggaatcgatg
    20941 gtgtggcgca taaagcggcc ttacaggtaa atggcgtcag cattgctgta ttggggaacg
    21001 gacttaatac cattcatccg cgccgccatg cccgactggc taccagtttg cttgaacatg
    21061 gtggggcact tgtctcggaa tttcccctcg atgttccacc ccttgcttac aatttcccac
    21121 gaagaaatcg cattatcagt ggtctaagta aaggtgtact ggtggtggaa gcggctttgc
    21181 gcagtggttc gttggtgaca gcacgttgtg cgcttgagca ggggcgtgaa gtttttgcct
    21241 tgccaggacc aatagggaat ccgggaagcg aagggcctca ctggttaata aaacaaggtg
    21301 cgattcttgt gacggaaccg gaagaaattc tggaaaactt gcaatttgga ttgcactggt
    21361 tgccagacgc ccctgaaaat tcattttatt caccagatca gcaagacgtg gcattgccat
    21421 ttcctgagct cctggctaac gtaggagatg aggtaacacc tgttgacgtc gtcgctgaac
    21481 gtgccggcca acctgtgcca gaggtagtta ctcaactact cgaactggag ttagcaggat
    21541 ggatcgcagc tgtacccggc ggctatgtcc gattgaggag ggcatgccat gttcgacgta
    21601 ctaatgtatt tgtttgaaac ctatattcac acagaagctg agttgcgtgt ggatcaagac
    21661 aaacttgaac aggatcttac cgacgcagga tttgatcgag aagatatcta caatgccctg
    21721 ctttggctgg aaaagcttgc ggattatcag gaaggactga cagaaccgat gcaactggcc
    21781 tctgatccac tctccatgcg tatttatacc cctgaggagt gtgaaagact ggatgccagc
    21841 tgtcgtggtt ttctgctttt ccttgagcaa attcaggtgc ttaaccttga aatccgtgaa
    21901 atggtgatag agcgagtgct cgcgctggat accgcagagt tcgacctgga agacctgaaa
    21961 tgggtaatcc tgatggtgtt gttcaatatt ccgggctgcg aaaatgcgta ccagcaaatg
    22021 gaagaattac tctttgaagt gaatgaaggt atgctgcatt aatcatttct ttaattcagc
    22081 ataagttgtt atggcgaaat cagcactgtt cacggtgcgt aataatgagt cctgctcaaa
    22141 gtgcggggct gaactggtta ttcgatccgg gaaacacggt ccgtttcttg gatgctcaca
    22201 gtatccggcg tgtgactacg tccgtcctct gaaatcttca gcggatggac atattgtcaa
    22261 agttctggag gggcaggttt gccctgcatg tggcgcaaat ctggtattac gccagggacg
    22321 ctttggtatg tttattggtt gcagtaacta ccctgaatgc gaacataccg aacttatcga
    22381 taaaccggac gaaacagcaa ttacatgccc ccaatgtcgg acgggccatc tggtccagcg
    22441 ccgctcccgt tatggcaaaa catttcactc ctgtgatcgc tacccggagt gtcaatttgc
    22501 cattaacttc aaacccatag ctggagaatg ccctgagtgt cattatccac tactcatcga
    22561 aaagaaaacc gcgcagggtg taaaacactt ttgtgccagt aaacaatgtg gaaagccggt
    22621 ttcggcggaa taataacgtg aataataacc tgcaaggaga cgctatcgca gctgcgatag
    22681 atgtcctcaa tgaagaacgt gtcatcgcct atccaacgga agccgttttc ggtgttgggt
    22741 gcgatcctga tagcgaaaca gcagtgatga gactgttgga gttaaaacag cgtccggttg
    22801 ataaggggct gattttaatc gcggcaaatt acgagcagct taaaccctat attgatgaca
    22861 ccatgctgac tgatgcgcag cgtgaaacca ttttttcccg ctggccaggt cctgtcacct
    22921 ttgtctttcc cgcgcctgcg acaacaccgc gctggttgac gggccgcttt gattcgcttg
    22981 ctgtacgagt caccgaccat ccgttggtgg ttgctttgtg ccaggcttat ggtaaaccgc
    23041 tggtttctac cagtgccaac ttgagtggat tgccaccttg tcgaacagta gacgaagttc
    23101 gcgcacaatt tggcgcggcg ttcccggttg tgcctggtga aacggggggg cgtttaaatc
    23161 cttcagaaat ccgcgatgcc ctgacgggtg aactgtttcg acaggggtaa cataatggaa
    23221 acctatgctg tttttggtaa tccgatagcc cacagcaaat cgccattcat tcatcagcaa
    23281 tttgctcagc aactgaatat tgaacatccc tatgggcgcg tgttggcacc catcaatgat
    23341 ttcatcaaca cactgaacgc tttctttagt gctggtggta aaggtgcgaa tgtgacggta
    23401 ccttttaaag aagaggcttt tgccagagcg gatgagctta ctgaacgggc agcgttggct
    23461 ggtgctgtta ataccctcaa gcggttagaa gatggacgct tgcagggtga caataccgat
    23521 ggtataggtt tgttaagcga tctggaacgt ctgtctttta tccgtccggg tttacgtatt
    23581 ctgcttatcg gcgctggtgg agcatctcgc ggcgtactac taccactcct ttccctgaac
    23641 tgtgcggtga caatcactaa tcggacggta tcccgtgcgg aagagttggc taaattgttt
    23701 gcgcacactg gcagtattca ggcgttgggt atggacgaac tggaaggtca tgagtttgat
    23761 ctcattatta atgcaacatc cagtggcatc agtggtgata ttccggcgat cccgtcatcg
    23821 ctcattcatc taggcattta ttgctatgac atgttctatc agaaaggaaa aactcctttt
    23881 ctggcatggt gtgagcagcg aggctcaaag cgtaatgctg atggtttagg aatgctggtg
    23941 gcacaggcgg ctcatgcctt tcttctttgg cacggtgttc tgcctgacgt agaaccagtt
    24001 ataaagcaat tgcaggagga attgtccgcg tgaatcaggc catccagttt ccggacaggg
    24061 aagagtggga cgagaataaa aaatgtgtat gttttcccgc tctcgtgaat ggtatgcaac
    24121 tgacatgcgc gatctctggc gagagtctgg cgtatcgctt tactggagat acgccagaac
    24181 agtggttagc gagttttcgt cagcatcgct gggacctgga agaagaagcg gaaaacttaa
    24241 ttcaggaaca aagtgaagat gatcaaggct gggtctggtt accctgatcc agatattcgt
    24301 ccttccattt cacgtaatta ttcgcggaat agcgtaaccc agccttctct tcatcactta
    24361 acgggcggat ctgtttgacg gggctaccga gatacagata tccgctctcc agccgtttat
    24421 tttgtgggac cagactaccc gcaccaatca tcacatcatc ttctactatt gcgccatcaa
    24481 gtaaaattga gcccatccca accaaaactc gattgccaat ggtgcagccg tggagcatca
    24541 ccttgtgacc aacagtgaca tcttcgccaa tggttaatgg gttgccatct gggttgtacg
    24601 aggatttatg agtgacatgc aacatactgc catcctggat attggtgcgt gctccgatct
    24661 gtacataatg tacatctcca cgaatcacaa cgagcggcca gatccccaca tcatcagcca
    24721 gacgaacgtc accaatcacg acactgctat cgtcgatcat tacgcgctga ccgatttgtg
    24781 gaaaaagatc gcggtatggg cgtaaaacat cagacatact tacctcagca ataaatgatt
    24841 tactaatgac tttggggaca ttattggcct tgtgcaagtc ttttagtatg caaaaaagca
    24901 ccgttttgtg tgcgattgca gcaaaaaggg tgaaaaaaca acaaacagaa aaaaagatca
    24961 aaaaaatact tgtgcaaaat attgggatcc ctataatgcg cctccgttga gacgacaacg
    25021 tgaaacactt cacaggatgg tcggaacaac gaagagaaaa aatcctgaaa ttcagggttg
    25081 actctgaaag aggaaagcgt aatatacgcc acctcgcgac agtgagctga agg
//
LOCUS       DAJTNA010000043        24852 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTNA010000043 DAJTNA010000000
VERSION     DAJTNA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24852
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.044"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..672)
                     /locus_tag="OO161_001489"
     CDS             complement(<1..672)
                     /locus_tag="OO161_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCR5462617.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAVHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(694..2706)
                     /gene="hyfR"
                     /locus_tag="OO161_001490"
                     /pseudo
     CDS             complement(694..2706)
                     /gene="hyfR"
                     /locus_tag="OO161_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            complement(2736..3149)
                     /gene="hyfJ"
                     /locus_tag="OO161_001491"
     CDS             complement(2736..3149)
                     /gene="hyfJ"
                     /locus_tag="OO161_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5462618.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3142..3900)
                     /gene="hyfI"
                     /locus_tag="OO161_001492"
     CDS             complement(3142..3900)
                     /gene="hyfI"
                     /locus_tag="OO161_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5462619.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3897..4436)
                     /gene="hyfH"
                     /locus_tag="OO161_001493"
     CDS             complement(3897..4436)
                     /gene="hyfH"
                     /locus_tag="OO161_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5462620.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            complement(4446..6161)
                     /gene="hyfG"
                     /locus_tag="OO161_001494"
     CDS             complement(4446..6161)
                     /gene="hyfG"
                     /locus_tag="OO161_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR5462621.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQDIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6151..7743)
                     /gene="hyfF"
                     /locus_tag="OO161_001495"
     CDS             complement(6151..7743)
                     /gene="hyfF"
                     /locus_tag="OO161_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5462622.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVQMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            complement(7748..8398)
                     /gene="hyfE"
                     /locus_tag="OO161_001496"
     CDS             complement(7748..8398)
                     /gene="hyfE"
                     /locus_tag="OO161_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5462623.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8410..9517)
                     /locus_tag="OO161_001497"
                     /pseudo
     CDS             complement(8410..9517)
                     /locus_tag="OO161_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(9529..9639)
                     /locus_tag="OO161_001498"
     CDS             complement(9529..9639)
                     /locus_tag="OO161_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR5462624.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9656..10600)
                     /gene="hyfC"
                     /locus_tag="OO161_001499"
     CDS             complement(9656..10600)
                     /gene="hyfC"
                     /locus_tag="OO161_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5462625.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLTPLFTGIARQIRARMHSRRGP
                     GIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGGG
                     DLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAG
                     STHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEY
                     SGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFVL
                     ASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10611..12628)
                     /gene="hyfB"
                     /locus_tag="OO161_001500"
                     /pseudo
     CDS             complement(10611..12628)
                     /gene="hyfB"
                     /locus_tag="OO161_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(12628..13245)
                     /gene="hyfA"
                     /locus_tag="OO161_001501"
     CDS             complement(12628..13245)
                     /gene="hyfA"
                     /locus_tag="OO161_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5462626.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQADGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCNFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(13498..13968)
                     /gene="bcp"
                     /locus_tag="OO161_001502"
     CDS             complement(13498..13968)
                     /gene="bcp"
                     /locus_tag="OO161_001502"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5462627.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(13968..14540)
                     /locus_tag="OO161_001503"
     CDS             complement(13968..14540)
                     /locus_tag="OO161_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5462628.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14686..15564
                     /gene="dapA"
                     /locus_tag="OO161_001504"
     CDS             14686..15564
                     /gene="dapA"
                     /locus_tag="OO161_001504"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5462629.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15581..16615
                     /gene="bamC"
                     /locus_tag="OO161_001505"
     CDS             15581..16615
                     /gene="bamC"
                     /locus_tag="OO161_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5462630.1"
                     /translation="MAYSVQKSRLAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            16828..17541
                     /gene="purC"
                     /locus_tag="OO161_001506"
     CDS             16828..17541
                     /gene="purC"
                     /locus_tag="OO161_001506"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5462631.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     ELKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17709..18572
                     /locus_tag="OO161_001507"
     CDS             17709..18572
                     /locus_tag="OO161_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5462632.1"
                     /translation="MRWQGRRESDNVEDRRNSAGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            18587..20602
                     /gene="tmcA"
                     /locus_tag="OO161_001508"
     CDS             18587..20602
                     /gene="tmcA"
                     /locus_tag="OO161_001508"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5462633.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGCGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAL
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            20676..21374
                     /gene="ypfH"
                     /locus_tag="OO161_001509"
     CDS             20676..21374
                     /gene="ypfH"
                     /locus_tag="OO161_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5462634.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFHGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21484..21684)
                     /locus_tag="OO161_001510"
     CDS             complement(21484..21684)
                     /locus_tag="OO161_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5462635.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21712..22839)
                     /gene="dapE"
                     /locus_tag="OO161_001511"
     CDS             complement(21712..22839)
                     /gene="dapE"
                     /locus_tag="OO161_001511"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5462636.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(22843..23199)
                     /locus_tag="OO161_001512"
     CDS             complement(22843..23199)
                     /locus_tag="OO161_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5462637.1"
                     /translation="MVTLYGIKNCDTIKKARRCLEAYNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23580..23639
                     /gene="ypfM"
                     /locus_tag="OO161_001513"
     CDS             23580..23639
                     /gene="ypfM"
                     /locus_tag="OO161_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5462638.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23738..>24852)
                     /locus_tag="OO161_001514"
     CDS             complement(23738..>24852)
                     /locus_tag="OO161_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCR5462639.1"
                     /translation="PAISGLGSSAGFDMELQDHAGAGHDALMAARNQLLALAAENPEL
                     TRVRHNGLDDSPQLQIDIDQRKAQALGVAIDDINDTLQTAWGSSYVNDFMDRGRVKKV
                     YVQAAAPYRMLPDDINLWYVRNKDGGMVPFSAFATSRWETGSPRLERYNGYSAVEIVG
                     EAAPGVSTGTAMDIMESLVKQLPNGFGLEWTAMSYQERLSGAQAPALYAISLLVVFLC
                     LAALYESWSVPFSVMLVVPLGVIGALLATWMRGLENDVYFQVGLLTVIGLSAKNAILI
                     VEFANEMNQKGHDLFEATLHACRQRLRPILMTSLAFIFGVLPMATSTGAGSGGQHAVG
                     TGVMGGMISATILAIYFVPLFFVLVRRRFPLKPRPE"
BASE COUNT         6257 a         6576 c         6588 g         5431 t
ORIGIN      
        1 atggcgaatg gcaatggcga acggaatcac aaacaaattg gcgatacagt gctcaaagcc
       61 actggcgaca aacagggtga tgggcaaaat catggcgacg attttgtcgc ataacgaacg
      121 cccgcaataa ctcatccaca gcgccaggca aaccatcaga ttgcacataa tgccgaggct
      181 gacagattca gtaaatgtat gatgcatttt accctcggcg cagtgtaaaa ccgcgactcc
      241 ccacatcgcg ttttcactca tcaccagccc ggaaaaccag attaacaaac tgaaacaggc
      301 aatacctgcc agattaccac aggccaccag aagtgcgtta atcagccaag ttcgccaact
      361 aataacgccc cgactttttg ccatcaccgt cattaccgac gaggtgaaca ggctggtgcc
      421 gcaaaccgcc agcaaaataa agccgagtgt aaagcaaagg ccgcccacca gatgggttaa
      481 tgcctgtgaa gacggggcat cggcgataac ggaaaggtaa aaagtaaatc cgatcgccat
      541 aaacacgcca gcggacatcg ccattaaaaa gaccgacact ttactgcggg caattttatg
      601 gacggcaatc cgttcagcga ttgccgcttt tctggcgctc aactgcaagt cgaaagagag
      661 tttgtttctc atgcatactc ctgcaaaagc agattacaac acctcgcgaa ccgagatccc
      721 cagacgctgc attcgtgaca gcagcgtggt gcgcttcatc cctaatcgtg tcgccgcgcc
      781 acggggcccg gcaacaatgc cattggtttc tcgcaatacc tgaacaatgc gctgacgttc
      841 ttcttcgtca ttctctggcg tcgtcgggtg cagcaactgc gccattgaac tgcgaagcgc
      901 tgaatcttca tttagcgtcg gcagtaaacg gctttgtcgg acattaagat gtaaattcag
      961 actgttaccg cgagtcagca gtaccgctcg ctcaatcacg ttttccagct cgtgcacgtt
     1021 gcccggccag tcccacgaca tcaactggcg tagcgcctcg gtcgggatgg cgtcaattgc
     1081 gcgattcata tggcgcgcca ttttttgcgt gaaatgtttt gctaaaagag ggatatcttc
     1141 cggacggtcg cgtagcggcg gcaattccaa tgggaagaca ttcaggcgat aaaagagatc
     1201 gctgcgaaac tggcgatctt caaccatttg tcacaaatca cggttggtgg cggcaatgac
     1261 tctgacattc accgggatcg ttctactccc gccgagacgc tcaatctccc gttcctgcaa
     1321 tacgcgcagc agtttaggct gaagttctaa cggcagatcg ccaatttcat cgagaaacaa
     1381 cgtgccgcca tcggcaattt caaaacgacc acgatgtgta ttaatcgcac cagtaaacgc
     1441 ccctttgtca tgaccgaata actcactttc cagaagactg gcggggatgg cagcgcagtt
     1501 gattttgacc agcggtttgt cgcgtcgcgt gctaagttga tggatcgctc tggcgatcac
     1561 ctctttgccg gttccggttt caccacaaat caacaccgca ctgtcgctct tcgctacaat
     1621 atctacctgc tggagcaggt cttccattgc ctggctttga tagataatgt caccgatgcc
     1681 cagattcgaa aggagctgct cgctaagctg gtagttttct tgctgcaaac tttcctgcaa
     1741 atcggtcatg ctacgccagg catcggcatt accaacggca atagcgatgc gatcggctat
     1801 gtgttgtagt agctggcagt tttcctcact aaagagagtg gaagaggtat gcgccagcaa
     1861 caatgcaccc ggtgtatggt tgccaaaggt aagcggtata aggagcgcag attcgcagcc
     1921 gttagatgca agtaagagta ataacgggtc gcgttgccag agaaacagat cgtctgcacg
     1981 gtgcgtcaag gtcggttgtc gggtttgtag cgtttgtgtc agcaatacac tgtcgccagg
     2041 catattgcgt ggcagacacg cacaatgtga ggcagaaaga tcgctgcacc acaggctaaa
     2101 cttctcgttc tttcgatgat cgcccagtac catactgact gaagccagac cgaaaaaatg
     2161 atggatctca cgagcgacgt cagcgatcag atcgtcgaga tcaagatgtg acagcactgc
     2221 attagtaata tcaaccagta cccgatgatg atcgcgatct ttctccagca aacgcagcgc
     2281 cacctgttgt tcttcatcat tgagccatga ttgcagaacc ggagtgataa tgcgtgccag
     2341 ccagttaaac agtgtgcaat cctagggagt aaaacggctg ccgtgggtac gaataaagcg
     2401 tagctccgcc attccactac tgccgagaag gacggcgtga cgcgaataat cgaccatctc
     2461 gccctgatac tctgttgttg ccgtcacatc gctgccatca tcatggtagc ggtaatagaa
     2521 gttgcttttg ataacatgca gttcaataag ttgtaccggg ctgaacggcg gcggcatcgg
     2581 gatgaaggcg cgtaataaag acgccttacc gtgtttctgt gctaaccgct ccgcgagcat
     2641 tccgtttacc gcttcaattg ttattccttg tggcggggca aacatcgcct cgtctgacat
     2701 agccatcaac agtccttatt aatgagaaat ccttctcacg caatttttct ggccatcaga
     2761 tagatggccg gttcctgcac aatttcatcc aacagatgaa tgaattgaat gctacgcgcc
     2821 tgccacacag acgtcgcgtc atctgccagt agcgcgaatg cccgggtcaa cagggcggtg
     2881 tggctggcgt caataacaat ctcaccaaag gtcatcaccc ccagcatctt acgtcgggct
     2941 tgctcctctt cgacctgtgc aagccaatct tcgttttccg tcagtgggca gcggaaggcg
     3001 acattcagac aatcaatcac gccaacgtga tggccgatag ccagcgagta atacattacc
     3061 tgagagctgt gttccggcat ctcgtcgcta ctggcgacaa acttttttcg cagcgtccag
     3121 aaaacaattt ccccgcactc ttcagtcatg taacccccgg agtacgcgaa agagttgctg
     3181 aacgatacta ttgagacgtg gatcgtcggc atcgcgcaac caggtgttaa cccgatttcc
     3241 tgtaggatcg tcgcttaaat ggcgcaggag ccgatcgcaa atttctcgcc cctgacgata
     3301 gcccgccagc cgccgcgctt ctcgctcaat ggcgatacgc tgtgatggcg ggatctgtgg
     3361 ccacaacggt tgcatagtca ccccggtggg atcgcgataa tccacagcgt gaatcttctg
     3421 ttgcagcaaa ccgagcgcca cggcgaaacc gtgaatggtg gcggccggtg ttggcgggca
     3481 gccggggatc caaacatcaa tggggacaat ggtgtcgctg ccgccccaga cgctgtagag
     3541 atcgtggaaa ataccgccgc cgacaccgca cgcgccgtag gaaacacaga ttttatgatc
     3601 gggggcagac tcatacgccc gaagtgcagg catacgcatc gcccgggtga ctgcgccagt
     3661 aaataacaaa atatcggcgt gacgcggtga tgaaacaacc ttaatgccaa aacgttctgc
     3721 gtcgaatacg ggggtgatgg cagcaaaaat ttcgatttca caggcgttgc agccgccgca
     3781 atcgacgcga taaacgtagg ctgagcgacg gatatcctgt agtaaatgcc gcttcatctt
     3841 ttgcgtttgc tcatccagcg tgatgggctg gctgacatgt tgtgtaagca ctggactcat
     3901 agctgctcct tagccaccag cggtacatct gtatcgtcgt tgatcagcgt cgcgcgttgt
     3961 ttgcactccg ggcagacgct cgcttgcgcc cgcaacattt cgcggttttg tggcgcattt
     4021 tgttgctgtg ccaacaattc agcagccagt gcgacggttt tttgcggggc aaacgggcgt
     4081 tcgcaacggc tgcaacgttg tagatggaac gtcgcgcggg tatagagatc ggctttattg
     4141 gtgacggtca gttcaaagtt attggtaagc tggatggctc tggtcgggca cacttcttca
     4201 caacgtccgc agtaaataca acgccccaga tagagctgcc aggtgcgcga attttgctgg
     4261 tcgtcggtct ggatagtcag cgcatttgcc ggacaagcac aggcgccgca ggcaatacat
     4321 tggctgggca tcaggtccgg tttcccgcga aagcctgggc tgacctccag tggcgcgaag
     4381 ggatatttca ccgtcgcggt tccggcgcgc ataatagttt tcagtaactt cagcatcttc
     4441 tgtccttact tcagcggcga acggttacga tcaatgccgt agcgttcgat ctctttatac
     4501 ggcacggttt ttgactggcg cttgcgcaca tctaccagcg tcacacggtc ggtacaggag
     4561 tagcagggat caaggctacc gataatcagc ggtgcgtcag aaacggtatt gccgcgcagc
     4621 atgtaacgca acaccggcca gttggcgtag gtggcggcac ggcagcgcca gcggaacaat
     4681 ttttggttat caccgagcat gctccagtgc acgtcttcac cgcgtggcgc ttcaacaaag
     4741 cccagcgcga atgcgtgagg tttatagcta aagccttcgg tcagcagtgg ggtatccggc
     4801 atgttgtcga gggcaaattc cagcattgcc agcgaatcaa acgtctcttt gacacggacc
     4861 atcacgcggg agaaaacatc gccgccggtg aaggtaaaca gtgtttttgg aatattgccg
     4921 tagtcggcgt aggggtgatc aaagcgcaaa tcacgggcaa aaccactgcc gcggatcagc
     4981 ggccctacag ggctataatc acgggcgatt tgtcggtcga gaatgccaat gtcctgagtg
     5041 cgttgttcca tattcggcgt agcaagcagc atctctacca gctccgacac gtcggcgcgc
     5101 atctcgcgca ccagtttcag cgtttgcaga cgttgctctt tgagaatatc gcggcgaaca
     5161 ccaccaatca gattcagacc gtaggtttta cgcgacccgg tcagcaattc cgccatcgtc
     5221 atcgactttt cccgcacgcg gaaaaattgc ataaagccgg tatcaaaacc aacaaaatgg
     5281 caggagaggc caaggttgag caaatgactg tgtagccgtt cgacttccag cagaatcgag
     5341 cgaatggtat gcgctcgttg cggcacctca atccccagtg cattttcaac cgagttggta
     5401 taggccacac tgtgggcaaa accgcaaatc ccacacacgc ggtccgataa gaaggtcact
     5461 tcgttgtagc ccatccgcgt ttctgccagt ttctccatgc cgcgatggac ataaaacagg
     5521 cggtaatcag catcgacaat ttgctcgcca tccacaaaca agcggaagtg acccggttca
     5581 tcggaggtga tatgcagcgg gccgacaggg atcacccgcg catcgctgtt gccctcattg
     5641 ataaacgcat acgtttcgga atctgtcgtc ggttcagggc gcagtcgata atccatcgca
     5701 tctttgcgca gcggatgcat atcttccggc cagtcatcgg gcaacaccag gcgacgctga
     5761 tccggcaggc caaccggaat cagcccgtac atatcgcgaa tttctcgctc gccccagacc
     5821 gcggcaggga cgcgcggtgt gactgacgga aactcccgac tgtcggcatc gaccagtgcc
     5881 ttcaccacga tccagcattt ttcggcccct tccattgaaa gggcataata aaccgcgtaa
     5941 tgaccgttaa gtgtccgctc gtcgttgcca aacaggactg gaagccagcc atcatgttga
     6001 taataaagat aatgtacaac gtcaggcagc agattgattt tcaccgtaat ggtgacctgt
     6061 tcaggcgttt gtcgctcttc atccagcact gcgccgggga actgcgtttt cagggcggcg
     6121 agaatcgctt cgccacgatt tgacgatgaa ttaacgttca ctgtgtttct ccgaaacaga
     6181 tgcggtgcct gaaggcaaaa aatcatgcca ggtgctacgt tgtgcaggca ggtcgtgcgt
     6241 ccctgagagg actatagtgg aagcgcccgc caggatcctg atgacaggtt gtggaatatg
     6301 cgttcccatc gccagcatca tgaccagcag aatcaccatt ggcgaggtgg tcaaccagcc
     6361 gagatcaccc cggttaacgg cctgcggcgg tttcgccatt aacacccgcg cagccatctg
     6421 taccaggccc gccagcacca gcgttaacag caataacagc aggacgataa tcagcaggtg
     6481 attacgtgcc agtcctgcgg taacggtcat aaattcgcta agaaaaatgt tgaagggcgg
     6541 catccctgcc agcgccagcg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa
     6601 tggcatgatt ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag
     6661 tacattgccg gaaccgcaga acagcagcgt ttttgccaga ctgtggttta aggtgtgcag
     6721 cagcgcggca aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat
     6781 gttctccacg ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa
     6841 tgccgccacg gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct
     6901 gccgatggct tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag
     6961 caatacggcg gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag
     7021 ccaggcgtgc atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa
     7081 ggccaacagc attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa
     7141 tatcgccatt tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc
     7201 gaacagacca aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag
     7261 cgatgaacgc tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat
     7321 cgccgcccac atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa
     7381 cagatggaag aaaccgtagt aatcgcacag tgtaacgggt gaaagctcgc cgtgtgccac
     7441 ttcatgacgc atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat
     7501 ggcgaggaac aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc
     7561 tcctgcatca gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc
     7621 taagctatgt aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa
     7681 acagagcagc gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact
     7741 catctcatta tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc
     7801 atatttttct tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac
     7861 ctatttccac cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt
     7921 tttccatcag gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca
     7981 gcaggccaag cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca
     8041 tcggtagctt cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca
     8101 gtgccatcat tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg
     8161 cagcgtaagt catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc
     8221 agatcagcag ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca
     8281 ccagcgactg gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa
     8341 acagcgatgt cagcatcatc agtcccgcca gattatttac gatcatagaa ccgatcattc
     8401 atcatctcct tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat
     8461 cagtacaatc aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgacggcctc
     8521 gctcggtttg ccaggcacaa cgcgaccaaa ccagcgaata aaccaggcga aactggcgac
     8581 cgattcaatc atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc
     8641 aaaaccggcg gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt
     8701 aatcgccagc gctgccacgc agaaaaccac gcctggcaac ggcagggtgt gcaatacgcc
     8761 acgcagacgc ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa
     8821 aaacaggctt ttagcgaacg cgtggttgac gatgtaggcg atgctgccct ccagtgccag
     8881 ccgcccactt tcaccatcga tgcggcgtgg agataggcgc tgatcggtgt tggcgcttcc
     8941 attgcgtccg gtagccacgc ttgcatcggt agctgggccg atttccccca cgcggcaaac
     9001 aggatgccgc cataaaccag ataacgtgcg tcgccatgta actcgctcat cgcgctaagc
     9061 acaaacgttc cggtctgcaa aaacagcgtg gcggcggcaa gatacaaccc caacgaaccg
     9121 atatgggtga taagcaacgc ttttagtgtt gaacgctgcg ctttatcgct ctggtaataa
     9181 ctgatcaacg cccaggagca gccgccctaa tttcaaaaaa caacaactga ccgagcagcg
     9241 tcgaggagag taccagtccc gccatcgcgc cgataaacac cagcaaaaat gcgtaataac
     9301 gattcgtgcc gttatgcggg tgttcgcgat ttttatccgt cagataaccc gtggagtaga
     9361 tcgtgaccag caaaccgaga aacaccacca caaacagaat cagcgtactc acgcgatcaa
     9421 tgaccaggcc aaacaatgcc acatccccca cgttgaccaa cgtcaacgtg acggcaactt
     9481 tatcggcctg ataaaacgcg gagatcagcg acaacatgca cagcgtggtc agtgcggcga
     9541 acaaaacccc cattcggcgg cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat
     9601 aaaaggcagc attaacgtcg tcagagcaag attttccata ttccgtcagt gctccttaca
     9661 gaccggttaa ccagaagacc catgcaagcg cagcaaggct gaagccaagc caggtcacat
     9721 ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag gccagtacaa
     9781 aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag gcgagagaaa
     9841 gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc atcacgacct
     9901 gtttcagccc cagcccccat ttcgctagcg ccagcccggc accggaatat tcggtcagcg
     9961 ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt cccatctcaa
    10021 tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc gagttccagc
    10081 ccgttgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc agcgccaata
    10141 ccagcagtga gagaatgagc atgggttcga ccagaatgcc gagcgtcaac tcgcgactgg
    10201 caccgactcc cgcaaacggg cttccggtat ccagcccgga aagagcaaag aaaaaacgaa
    10261 acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa ggggaaacgg
    10321 taataaaaag tggtagggcc atcgccagca ccagcatgct gctgattaat acccacggca
    10381 tcaggcggaa catcagacct gaagatatag gcgcaacttc ctggcgttta aacagtttgt
    10441 ggatctcgcg ataatcctgc cagatccccg gcccgcggcg ggagtgcata cgcgcgcgta
    10501 tctgccgggc aatacccgta aaaagcgggg ttagcagcag aatcacggcc tgtgctaacg
    10561 caaaaatgac cagatatccg tcgcaaagag tttgtctcat ggtgatttcc ttagacggca
    10621 atagcgatta gcagcacaac cagtgcggca actagataca ggcaatagag acgaaagtcg
    10681 ccgctttgca gatgctgtat ttctttggcc agccgttggg tggcgctcac gatggggcgg
    10741 atcacccgct catcccagaa gggttctgtg ctctgagccc tggcggtgac gtgcgcaaga
    10801 tctttattta gcctcagcgt agggtcgagc tgttgtcgta gacgaaataa cgcagagaag
    10861 accacacgca gcggctgcat cacgccattg cctgacggag ccatcgcgtt ttcccagccg
    10921 tagccgcatg cccaggcatc tcctgtgcga cgaaacgctg cgcggcgcga acgacaccac
    10981 agccagtaaa ggccaggcag caaaggcatc gccagtagta acagcaacag taatgaagga
    11041 gtgacccgtg tatgaaacgt gccgggtaca agtgctattc cgctggcgac agtgacggga
    11101 ggggtattgg taaacgcatg ggcaatatgc attatcttcg gtgccagcca actggcacta
    11161 agcgcaatta atacgcagag tgccgcgagc agtagcatgg cgacgatcat cgtatttggc
    11221 acttcctgtg cctcttcagc gtgtgtactg cgcggcgcac cacagaaagt aataccgtac
    11281 attttgacga agcacattac tgccagccca ccagtgactg ccagcattac catagcaata
    11341 ggacccgcaa gttgtagcgc tacggcttcc acacgactta gtgagaacag cgactgccag
    11401 gtgtaccatt cgctgataaa accattcagc ggaggaatgg ctgatatcgc gaggcaacca
    11461 atcaggcatg ctgcggctgt ccacggcatc cgtttcgcca gtgcccccat tttttccatg
    11521 tcgtgggtat gcaaacgcga aataatcgcg cccgcgccga gaaatagcag ccctttgaac
    11581 agcgcatggt tgagcagatg aaacagtgcg ccgagcagtc caaccacggt gagcagcggg
    11641 tcgtgcagtg acagaccgtc catcgccaca ccgactgcca gcaaaataat gccgacgttt
    11701 tcgacggtac tccaggccag cagccgtttg atatcctgtt ccgccagcgc atatagcacg
    11761 cccaggagcg cggagattgc gccgatcgcc atcaccagaa tgccccacca cagaggcaaa
    11821 cccgtttgcg ccagcagatc catcgctact ttcaggatgc cgaaaatacc gattttgacc
    11881 attacgccag acatcaacgc cgaagcgtgc gatggtgcgg cagggtgagc gcgcggcaac
    11941 cagctgtgca acggcatcat cccggctttc gcgccaaaac cgaaaaaggc cagcagaaac
    12001 accgccgacg ccagccccgg agaaagtgaa agcgtgcgaa actggcaaaa tcgaggctgc
    12061 cgctttcgcg ccccatcagc aagaaggcga tcataatcag caccgatccg gcgtgggcga
    12121 taaaaaagta gagcatgcca gcgttgatcg acgttttatc ctgcctggca atgaccagaa
    12181 accaggaaga cagcgacatc atttcaaaca gcacgatgaa ccaaaaagcg ttgtccatca
    12241 ccagcagggc aaccatcgat gcgatgaaaa gattcataaa gaagcccatc gccgccgcgc
    12301 ctttgccctc gtattcgcgc atataagtca atgaatagag cgaacaaacc accaccagca
    12361 aggagataac cagcaccata aatgccgaga ggctatctat tcgcagggtt aaatcggcaa
    12421 agtcaaaagg ggtggcaaaa cgggcgacta aagttacacc agcatgttat tgcgtaattc
    12481 cgctgatgat gccaatcacg ccgcccacca gcgatgtgat gccggaaagc ttaatagcca
    12541 gtctgtccag accgagtaaa aacagcgaag ccagactggc aaacagaaag aggatcagcg
    12601 accaggttaa taattgcagg gcatccatta gtgttgctcc tgagtgaggg aaaggggatc
    12661 ctccccgcca ttggcaaacc agcttgcggc aaggcgctgt ttttctttca tctgacgttg
    12721 caggctatcg tcggtaatca gccgtaacgc ctgattcggg caagcgcgaa cgcaggccgg
    12781 accttctggc aagaaattac acaggtcgca tttcaccgcg acggtctgga cacccggttc
    12841 ccagcgcagc aaagcatgtt gggtcggcgc gttttcttcg ccatctttga gtgagccgtc
    12901 agcctgaaaa acatattgcg caggggcatt caccggacgg cttcctgaag cgctgattgc
    12961 gccaaatggg cagaccacgg cgcaaagctt gcagccaata cagaggcttt cgttgagttg
    13021 gatcgcatca tccctctggg agatggcatt gaccgggcag acctgcaggc aaggggcttc
    13081 ctcacagtga tgacacacga caggggcagt gattgttgac gtcttcgcca gggccaggcg
    13141 cgggtgttgc tgtaaacctt gcgttttatg cacgtccgaa caggcagcga gacaggtatt
    13201 acatcctgta caccacagtg gttcggccac cacaaagcgg ttcatgtcgg ctccttgaaa
    13261 atgatatgaa acattgcatt tcatattgca taaaggatgc cagtctgatt aatttaatat
    13321 attcaataaa ttatgtatta actgggttct gaacggtaca aaaatgatgg tcattaacgt
    13381 tcagcaaaaa atggattcga cacccgtgtc gcaggggaaa gcgagaatgt gatcttgatc
    13441 acgagaagtg aggtgtgctg accgcaagcg cagccagcac ggaatggagc aaagtaatca
    13501 ggcgtgttct ttcagccagt tcagcacaac gtcgtggtga ttgctggttt taaaatcgtc
    13561 aaagacatgt tcgattttgc catcagcgtc aatcaggaag ctgatgcgat gaataccatc
    13621 gtaggttttg cccatgaagg acttttcacc ccagacgccg aattgttcgc acacctggtg
    13681 gtcctcatca gacaagagcg taaagttaag cagctctttt tccgcaaaac gggagagttt
    13741 ttcgggttta tcggtgctga tacccagcac atcaacgccc gcttttttca actcatccat
    13801 gttatcgcgt aagccgcagg cctgtacggt acagccgggg gtcatggctt tcgggtagaa
    13861 ataaaccaga acacgctgtc cctggaagtc ggtcaaatta acttgttctc cgtcttgatc
    13921 cggcaagcta aatttcggtg cgatatcacc ggctttcagt ggattcatta cttaactcca
    13981 tcctgttcat catgttggga ataattgacg acgttaatac tgccttgtgc attgagttct
    14041 gtacataggg ctttgaacgc ttgctcaata tttgctgcgt ccgcagatgc ggggctgtgg
    14101 gcggtgatct gaatatgcaa ctgcgcagcc cgttcatttt cagcaggttg cgtgcgcgac
    14161 accagctccg caatgttcat atgatgcgcg tcgaaaagtg ctgtgaagcg ttcaattaaa
    14221 tgcggggagt ctgcaacatc gacctgaacc cagacagatg ctggcattgg cggacgcgga
    14281 cgcgccgtcg tgcgcttcat cacgattaaa agatccagtt cggcaccttt caacggtaac
    14341 gttgattcaa tcagagtaat ggcattccat gaaccggaaa gcagcatgat aaacgtgaac
    14401 tcttctccca gcatcgccag gcgactgtct tcaatattac agccgcaact actgacatga
    14461 cgggtgatgg tgttcacaat tccagggcga tcggcaccca acgcagtgat caccagataa
    14521 tgttgcgatg acagtgtcaa actggttatt cctttaaggg gtgagttgtt cttaaggaaa
    14581 gcataaaaaa aacatgcata caacaatcag aacggttctg tctgcttgct tttaatgcca
    14641 taccaaacgt accattgaga cacttgtttg cacagaggat ggcccatgtt cacgggaagt
    14701 attgtcgcga ttgttactcc gatggatgaa aaaggtaatg tctgtcgggc tagcttgaaa
    14761 aaactgattg attatcatgt cgccagcggt acttcggcga tcgtttctgt tggcaccact
    14821 ggcgagtccg ctaccttaaa tcatgacgaa catgctgatg tggtgatgat gacgctggag
    14881 ctggctgacg ggcgcattcc ggtgattgcc gggaccggtg ctaacgctac tgcggaagcc
    14941 attagcctga cgcagcgctt caatgacagc ggtatcgtcg gctgcctgac ggtaacccct
    15001 tactacaatc gtccgtcgca agaaggtttg tatcagcatt tcaaagccat cgctgaacat
    15061 actgacctgc cgcaaattct gtataatgtg ccgtcccgta ctggctgcga tctgctcccg
    15121 gaaacggtgg gccgtctggc gaaagtaaaa aatattatcg gaatcaaaga ggcaacaggg
    15181 aacttaacgc gtgtaaacca gatcaaagag ctggtttcag atgattttgt tctgctgagc
    15241 ggcgatgatg cgagcgcgct ggacttcatg caattaggcg gtcatggggt tatttccgtt
    15301 acggctaacg tcgcagcgcg tgatatggcc cagatgtgca aactggcagc agaagggcat
    15361 tttgccgagg cacgcgttat taatcagcgt ctgatgccat tacacaacaa actatttgtc
    15421 gaacccaatc caatcccggt gaaatgggca tgtaaggaac tgggtcttgt ggcgaccgat
    15481 acgctgcgcc tgccaatgac accaatcacc gacagtggtc gtgagacggt cagagcggcg
    15541 cttaagcatg ccggtttgct gtaaagttta gggagatttg atggcttact ctgttcaaaa
    15601 gtcgcgcctg gcaaaggttg cgggtgtttc gcttatttta ttactcgctg cctgtagttc
    15661 tgactcacgt tataagcgtc aggtcagtgg tgatgaagcc tacctggaag cggcaccgct
    15721 tgcggagctt catgccccgg ctggaatgat tttgccggtg acctccggtg attatgcaat
    15781 cccggtgacc aacggtagtg gtgctgtcgg taaggcgctg gacattcgtc caccagccca
    15841 gccgctggca ctggtttctg gcgtgcgtac ccagttcacg ggcgataccg cttcattgct
    15901 ggtggaaaat ggtcgtggca atactctgtg gccgcaggtg gttagcgtgc tgcaggcgaa
    15961 aaactacacc atcacccaac gtgatgatgc tggtcagaca ctgaccatcg attgggtaca
    16021 atggaaccgt ctggacgaag acgagcagta tcgtggtcgt tatcaaatct ctgttaagcc
    16081 gcagggttat cagcaggcgg ttacggttaa actgctgaac ctggaacagg cgggtaaacc
    16141 ggttgcagac gcggcttcca tgcagcgtta cagcacggag atgatgaacg ttatttccgc
    16201 cggtctggat aaatctgcct ctgacgccgc gaacgctgcg caaaatcgtg cctccaccac
    16261 tatggacgta caaagtgcag ctgatgacac cggtttaccg atgctggtcg tacgcgggcc
    16321 gttcaatgtg gtttggcaac gtctgccagc ggcgctggaa aaagtgggca tgaaagtgac
    16381 cgacagcacc cgttcgcagg gtaacatggc cgtaacttat aagccgctgt ctgacagcga
    16441 ctggcaggaa ctgggcgcga gcgatccagg cctggcatcc ggtgactata aactgcaggt
    16501 tggcgattta gataaccgca gcagcctaca gttcatcgat ccgaaaggtc atactctgac
    16561 tcagagtcag aacgacgcgc tggtagctgt cttccaggct gcgtttagca agtaaaaata
    16621 cagggctgga gtcatccggc ccttttttct gatatgatac gcaaacgtgt gcgtcggcag
    16681 aaaaatgcaa ttttagcggt aattcgcacg aaatttgttt gtcggacgta ggtcggataa
    16741 ggcgttcttg ccgcatccga caaaacatcc ggcacaccag acaacaaaag attttaaaac
    16801 gttaattcac acccaggagt gataaagatg caaaagcaag ctgagttgta tcgtggtaaa
    16861 gcgaaaaccg tatacagcac ggaaaacccg gacctgttgg tgctcgaatt ccgcaatgat
    16921 acgtcagcag gggatggcgc gcgcattgag cagtttgatc gcaaaggtat ggtgaacaac
    16981 aagttcaact acttcattat gagcaaactg gctgaagcgg gtatcccgac tcaaatggag
    17041 cgtctgctct ccgataccga atgtctggtg aaaaagctgg atatggtgcc ggttgagtgt
    17101 gtcgtgcgta accgtgctgc tggctctctg gtgaaacgtc ttggaatcga agaaggtatt
    17161 gagctgaacc cgccgctgtt cgatctgttc ctgaaaaacg atgccatgca cgatccgatg
    17221 gtcaacgaat cttactgcga aacctttggc tgggtgagca aagagaacct ggcgcgtatg
    17281 aaagagctga cctacaaagc gaacgacgag ctgaaaaaac tgttcgatga tgctggtctg
    17341 attctggtcg acttcaagct ggagtttggt ctgtacaaag gtgaagtggt actgggcgat
    17401 gagttctccc cggacggcag ccgcctgtgg gacaaagaaa cactggagaa aatggacaaa
    17461 gaccgtttcc gccagagcct cggtggcctg atcgaagcct atgaagccgt cgcccgccgc
    17521 ctgggtgtac agctggactg atttttctgt tcatcatctt gccgtgcagc tggcacggca
    17581 agacaaccgc tctcggaata tatgcattga acgctcattc ctccgccatt tcatcccgct
    17641 ttcctgtggt aatcttcctc tgaaccacct acgatcattt ctataatgaa tatattgttg
    17701 aggtatctat gcgttggcaa gggcgacgtg aaagtgacaa tgttgaagac aggcgcaaca
    17761 gcgccggtgg tccgtctatg ggcggtcccg gttttcgcct gccaagcggt aaaggcgggc
    17821 tgattttact gatagtcgtg ctggttgcag gctactatgg tgttgattta accgggttaa
    17881 tgactggaca gccggtttcc caacaacaat caacgcggtc cattagccct aatgaagacg
    17941 aagctgcaaa attcacctcg gtgattctgg caaccacgga agacacctgg ggacaacagt
    18001 tcgagaagat gggtaagacc tatcagcaac cgaagctggt catgtaccgt ggaatgacgc
    18061 gcaccggctg cggggcgggc cagtccatta tggggccgtt ctattgcccg gcggatggca
    18121 cagtttatat cgatctctcc ttctatgatg acatgaaaga caaacttggc gcggatggcg
    18181 attttgccca ggggtacgtt atcgcccatg aagtcggtca tcatgtgcag aaactgttag
    18241 gcatcgagcc gaaagttcgt caactgcaac aaaacgcgac gcaggcggaa gtgaaccgct
    18301 tatctgtgcg tatggaactc caggccgact gttttgccgg tgtctggggg cataacatgc
    18361 agcagcaagg cgttctggaa accggcgatc tggaagaggc gctgaatgcg gcgcaggcca
    18421 tcggcgatga ccgtttacaa cagcaaagtc aggggcgagt agtaccagac agtttcacac
    18481 atggcacttc tcagcaacgc tacagctggt ttaaacgtgg tttcgacagc ggcgatccgg
    18541 cacaatgcaa tacttttggt aaaagcattt aatttccggg gcagggatgg ctgaactgac
    18601 tgcgctttac acattaacag cgcaaatgaa acgtgaaggg atccgccgct tgctggtgtt
    18661 gagcggggaa gagggttggt gttttgatca tgcgcttaag ttgcgtgatg ccttacttgg
    18721 cgactggctg tggatttcgc cgcagccaga tgctgaaaac cactgttctc cctcggcgct
    18781 acaaacttta cttgggcgcg agttccggca tgcggtattc gacgcccgcc acggctttga
    18841 tgccgctgcc tttgcggcac ttagcggaac gttgaaagcg ggaagctggc tggttttgtt
    18901 actccctgta tgggaagagt gggaaaacca acctgatgcc gactcgctgc gctggagtga
    18961 ttgccctgac cctattgcga cgccgcattt tgtccagcat ctcaaacgcg tacttacggc
    19021 ggataacgac gctattctct ggcggcaaaa ccagccgttc tcgttggcgc attttactcc
    19081 ccgtactgac tggcaccccg cgaccggcgc accacaacca gaacaacagc aactcttaca
    19141 gcagctactg accatgccgc cgggcgtggc agcggtaacg gctgcgcgtg ggtgcggtaa
    19201 gtcggcgctg gcagggcaac tcatttctcg tattgcgggc agtgcgattg tcaccgcgcc
    19261 cgcaaaagcg gcaacggatg tactggcaca atttgcgggc gagaagtttc gctttattgc
    19321 gccggatgcc ttgttagcca gcgatgagca agccgactgg ctggtggtcg atgaagccgc
    19381 agccatacct gcgccgttgt tgcatcaact ggtatcgcgt tttcctcgaa cgttgttaac
    19441 cactacggtg cagggctacg aaggcaccgg acgtggtttt ttgctgaaat tttgcgctcg
    19501 ctttccgcat ttacaccgtt ttgaactgca acagccgatc cgctgggcac agggatgccc
    19561 gctggaaaaa atggttagtg aggcactggt ttttgacgat gaaaacttca cccatacacc
    19621 acaaggcaat atcgtcattt ccgcatttga acaaacgtta tggcgaagcg agccagaaac
    19681 gccgttaaag gtttatcagt tattgtctgg tgcgcactac cggacttcgc cgctggattt
    19741 acgccggatg atggatgcac tagggcaata ttttttacag gcggctggcg aaaacgagat
    19801 tgccggagcg ctgtggctgg tggatgaggg gggattatct caacaactca gtcaggcggt
    19861 atgggcaggt tttcgtcgcc cgcggggtaa tctggtggcc cagtcgctgg cggcgcacgg
    19921 tagcaatcca ctggcagcga cattgcgtgg acggcgggtc agccggatag cagttcatcc
    19981 ggctcgtcag cgcgaaggcg ttgggcaaca gctcattgcc agcgctttgc aatatacgca
    20041 agacctcgac tatctttcgg tgagttttgg ttacaccggg gagttatggc gtttctggca
    20101 acgctgcggt tttgtgctgg tacgaatggg taatcatcgt gaagccagca gtggttgcta
    20161 tacggcgatg gcgctgttac cgatgagtga tgcgggtaaa cagctggctg aacgtgagca
    20221 ttaccgttta cgtcgcgatg cgcaagctct cgcgcagtgg aatggcgaaa cacttcctgt
    20281 tgatccacta aacgatatcg tgctttctga cgacgactgg cttgaactgg ccggttttgc
    20341 ttttgctcat cgtccgctat taacgtcgtt aggttgctta ttgcgtctgc tacaaaccag
    20401 tgaactggca ttaccggcgc tgcgtgggcg tttacagaaa aatgccagtg atgcgcagtt
    20461 atgtaccaca cttaaacttg caggccgcaa gatgttactg gtccgtcagc gggaagaggc
    20521 cgcgcatgcg ctgttcgcac ttaatgatgt tcgcactgag cgtctgcgcg atcgcataac
    20581 gcaatggcaa ttttttcact gactccttca attttctccc atggtcattg tgagccagcg
    20641 gcgtaaaatt aatgacatca gttaaggagt tcgccatgaa acatgaccat tttgttgttc
    20701 aaagcccgga taaacctgtg caacagttgt tactgctttt ccacggtgtc ggggataact
    20761 cggtggcaat gggggaaata ggcagttggt ttgcaccgct gtttcctgat gcgctggtgg
    20821 tgagtgtcgg tggcgcggag ccgagcggta atccggcggg gcgtcagtgg ttttcggtgc
    20881 agggtattac ggaagataat cgccaggcgc gtgtggatgc catcatgccg acgtttattg
    20941 aaacggtacg ctactggcag aaacagagtg gagtaggggc taacgccacg gcgttaatcg
    21001 gtttctctca aggcgcgatt atggcgctgg agagtattaa agccgaaccg ggccttgctt
    21061 cacgcgtcat tgcttttaac ggacgctatg cgagcctgcc ggaaaccgcg tctacagcaa
    21121 cgacaattca cctgattcat ggcggtgaag atccggttat cgatctggcg catgcggttg
    21181 cggcgcagga agcgttaatc agtgccggtg gtgacgtgac gctggatatc gtggaggatt
    21241 taggccatgc gatcgacaat cgcagtatgc aatttgccct ggatcacttg cgttatacca
    21301 ttccgaaaca ttatttcgac gaagcgctaa gtggcggtaa gcctggcgat gatgatgtga
    21361 ttgaaatgat gtaaaagaca gattacctgg cctgataaga cgcgtgagcg tcgcatcagg
    21421 catcgttcac cattgccgga tgcggcgtta acgccttatc cggcctacgg ttgagcactc
    21481 agcttacttc ttcggttgat cctttttcgg ccagtcgtcg tcatcgtccc acttatcgtt
    21541 gaaatcacga tgcgggggaa gctccggctt gttggcgaga aatttcttgt ggtcgacgcg
    21601 cttgagatct ttaatcacgt tcagcaggac gcctaccaaa aagacaatca ccagaatcca
    21661 ccaatattta gccagccagt ccatgcttat ttcctcttgc agaaccactc atcaggcgac
    21721 gagctgttcc atgatacgtt gatacatacg ggcaagtagc tgcaggtcgg cagcgttcac
    21781 acattcatta attttatgaa tagtggcatt gaccggcccg agttccacca cctgcgcccc
    21841 catgcgagca ataaagcgcc cgtcggacgt tccgcctgtg gtcagtagct gcggtttaat
    21901 ttcattatag tgctcaaccg cgttaacgac cgcatccacc agtttaccgc gcgcggtcaa
    21961 aaatggctgc ccggaaagcc accaatccac cgtatagcgc agttgatgtt tttcaagcag
    22021 ggcaagcacc tgcgctttga tcatctcatc agtcagttcg gtgctgaagc ggaagttaaa
    22081 ctgcacaaac agttcacccg gaataacatt gttactgccc gttcccgcct gaatattagc
    22141 aatctgcata ctggtcgccg ggaaaaattc attgccctga tcccactcta tagccactaa
    22201 ttcattaagg aaaggtgctg cgcgatgtac tggattgtca gccagatgcg ggtaggccac
    22261 atgcccctga acgccatgaa tggtaaggtt gcaggttaac gatccacgac gaccattttt
    22321 caccacatca ccaaccactt cgatactcga cggttcgccg accaggcagt aatcgagacg
    22381 ctcattacgt gccatcaacg cttcgacgac ttttaccgta ccgttgtggg cactggcttc
    22441 ttcatcagag gtgatcagaa atgccagtcg ccccgtatgg ttgggatgtt gtgcgacaaa
    22501 gcgttctgcc gctaccacca tcgccgccag cgagcctttc atatctgccg caccgcgccc
    22561 gaataacatg ccatcacgaa tggtgggttc aaacggcgga ttgatccaac gatcggcgtc
    22621 gccaggcggc accacgtcgg tatgcccggc aaaggctaac gtttcacctt gcccacgcca
    22681 tgcccaaaaa ttctgcgtat cggcaaagtc catgcgttca acggtaaaac cgatcgcctg
    22741 caaacgttca atcaacaaag cctggcatcc tgcgtcatca ggactcaggg aagggcggcg
    22801 aataagctgt tgtgtcagct caataaccgg gcacgacata gactacacct catggaaaaa
    22861 ttgctgataa ctggaatcac tgaaacccag cagcataggc ttaccaggcg cgcagagcaa
    22921 tggacgtttg ataattgaag gcatttcagt cattaatgcc gccgcagagg ccgcatcggt
    22981 gattttattg cgggtggttt cgtccagttt acgccaggtt gtaccacggg tgttcagtaa
    23041 cgcttcccag cctaattcgt tgataaaacc gttcaataat tcgctgtcca gcccatcgac
    23101 gcggtaatca tgaaaacgat agtcgatgtt ataggcttcc agacaacggc gagccttttt
    23161 aatggtgtca caatttttga tgccgtaaag tgtaaccata atgaatcctt tttcgatcta
    23221 tgagaataaa tactcattta agggtgaaaa cattttgcct gcaaatgatt atcaggaaat
    23281 ataacattat cgaagtattc aaattaacaa ctattaaata atattaaaca tttttaacgt
    23341 aaacattaag aatattcatc ttgttatctg tctgtatttt aaccagaatt aaatcagtat
    23401 tagatgccgt atgaatatct cgaaatgttg ctgtgtatca catacaaatt atcaccgcag
    23461 gaccatagta tttacataag gtttgtcccc cggaggtagt catcgcagca atcgagtaaa
    23521 ttagcttcac cacggtatat tgtttccgta gaatacccat aattataaga gaggttgtta
    23581 tgattgaacg tgaactgggg aactggaaag actttatcga agttatgctt cgtaagtaat
    23641 tcctgagaca aagtacaaac agcaagaacc cgctgagcag gttcttaatc ggaaagttga
    23701 aaaaggcgac acattagcat gtcgcctttt ttattgctta ttccgggcgc ggcttcagcg
    23761 ggaagcggcg gcgcaccagt acaaagaaca gcggcacgaa gtaaatagcc agaatagtgg
    23821 ccgaaatcat cccgcccatt acgccagtac ccaccgcatg ctgaccaccg gaaccggcac
    23881 ccgtgctggt tgccattggc aatacgccga agataaatgc cagcgaggtc atcagaatcg
    23941 ggcgtaaacg ctgacggcag gcgtggagcg tcgcttcaaa caggtcgtgg cctttttggt
    24001 tcatctcgtt agcaaactcg acgatcagga tggcgttttt cgccgataaa ccaatgaccg
    24061 ttaacaggcc cacctggaag taaacgtcgt tttccagccc gcgcatccag gttgccagca
    24121 gcgcgccgat aacccccagc ggcacgacca gcattaccga gaacggcacc gaccagcttt
    24181 catacaacgc cgccagacac aggaatacca ccagcaagga aatggcgtac agcgccggag
    24241 cctgcgcgcc ggaaagccgc tcctgatacg acatcgccgt ccactccaga ccaaagccgt
    24301 ttggcaactg cttcactaac gattccatta tatccatcgc cgtaccggta ctgacccccg
    24361 gtgcggcttc cccaacaatc tcaaccgcag aataaccgtt atagcgttcc agacgcggcg
    24421 agccagtctc ccagcgtgag gtcgcgaaag cagagaaggg caccatgccg ccatctttat
    24481 ttcggacata ccagagatta atgtcatctg gcagcatgcg atacggcgca gctgcctgca
    24541 catagacttt cttcacacga ccgcgatcca taaagtcatt cacatagctc gaaccccagg
    24601 cggtttgcag tgtgtcgtta atatcgtcga tagcaacgcc cagcgcctga gctttacgct
    24661 ggtcgatatc aatctgcaac tgcggactgt catcgaggcc gttatggcgc acacgggtta
    24721 gctccgggtt ttccgccgcc agcgccagca actggttacg tgctgccatc agcgcatcgt
    24781 gacctgctcc agcgtggtcc tgcaactcca tatcaaaacc cgcagaacta ccaagaccgc
    24841 taattgctgg cg
//
LOCUS       DAJTNA010000044        24655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTNA010000044 DAJTNA010000000
VERSION     DAJTNA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24655
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.045"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..808
                     /locus_tag="OO161_001515"
     CDS             <1..808
                     /locus_tag="OO161_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR5462640.1"
                     /translation="PRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTG
                     GRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVIN
                     LTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAF
                     AMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHN
                     GFSYWIYGCMGVLAALFMWKFVPETKGKTLEELEALREPETKKTQQTATL"
     gene            complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OO161_001516"
     CDS             complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OO161_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5462641.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            1520..1798
                     /locus_tag="OO161_001517"
     CDS             1520..1798
                     /locus_tag="OO161_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462642.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSFRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(1845..3941)
                     /locus_tag="OO161_001518"
     CDS             complement(1845..3941)
                     /locus_tag="OO161_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5462643.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLDQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3941..4678)
                     /locus_tag="OO161_001519"
     CDS             complement(3941..4678)
                     /locus_tag="OO161_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5462644.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(4675..5313)
                     /locus_tag="OO161_001520"
     CDS             complement(4675..5313)
                     /locus_tag="OO161_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5462645.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(5427..5669)
                     /locus_tag="OO161_001521"
     CDS             complement(5427..5669)
                     /locus_tag="OO161_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462646.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OO161_001522"
     CDS             complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OO161_001522"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5462647.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            8305..9654
                     /gene="lysC"
                     /locus_tag="OO161_001523"
     CDS             8305..9654
                     /gene="lysC"
                     /locus_tag="OO161_001523"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5462648.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            9906..10178
                     /locus_tag="OO161_001524"
     CDS             9906..10178
                     /locus_tag="OO161_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5462649.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OO161_001525"
     CDS             complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OO161_001525"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5462650.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            11531..12478
                     /locus_tag="OO161_001526"
     CDS             11531..12478
                     /locus_tag="OO161_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5462651.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            12548..13354
                     /locus_tag="OO161_001527"
     CDS             12548..13354
                     /locus_tag="OO161_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5462652.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWSTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVTDFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            13364..13771
                     /locus_tag="OO161_001528"
     CDS             13364..13771
                     /locus_tag="OO161_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5462653.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLGKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            13771..14265
                     /locus_tag="OO161_001529"
     CDS             13771..14265
                     /locus_tag="OO161_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5462654.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYQNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            14331..15128
                     /locus_tag="OO161_001530"
     CDS             14331..15128
                     /locus_tag="OO161_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5462655.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            15139..15960
                     /locus_tag="OO161_001531"
                     /pseudo
     CDS             15139..15960
                     /locus_tag="OO161_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            16015..17244
                     /gene="sorE"
                     /locus_tag="OO161_001532"
     CDS             16015..17244
                     /gene="sorE"
                     /locus_tag="OO161_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5462656.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHSSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            17627..18316
                     /gene="pepE"
                     /locus_tag="OO161_001533"
     CDS             17627..18316
                     /gene="pepE"
                     /locus_tag="OO161_001533"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5462657.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(18407..20038)
                     /locus_tag="OO161_001534"
     CDS             complement(18407..20038)
                     /locus_tag="OO161_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5462658.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(20258..23408)
                     /locus_tag="OO161_001535"
                     /pseudo
     CDS             complement(20258..23408)
                     /locus_tag="OO161_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            23608..24432
                     /gene="iclR"
                     /locus_tag="OO161_001536"
     CDS             23608..24432
                     /gene="iclR"
                     /locus_tag="OO161_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5462659.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         5826 a         6483 c         6428 g         5918 t
ORIGIN      
        1 tcctcgctgg ctgatgtcac gcggcaagca agaacaggcg gaaagtatcc tgcgcaaaat
       61 tatgggcaac acgcttgcaa ctcaggcagt acaggaaatt aaacactccc tggatcatgg
      121 ccgcaaaacc ggtggtcgtc tgctgatgtt tggcgtgggc gtgattgtca tcggcgttat
      181 gctctccatc ttccagcaat ttgtcggcat caatgtggtg ctgtactacg cgccggaagt
      241 gttcaaaacg ctgggggcca gcacggatat cgcgctgttg cagaccatta ttgtcggagt
      301 tatcaacctc accttcaccg ttctggcaat tatgacggtg gataaatttg gtcgtaagcc
      361 actgcaaatt atcggcgcac tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt
      421 ttacactcag gcaccgggta ttgtggcgct actgtcgatg ctgttctatg ttgctgcctt
      481 tgccatgtcc tggggtccgg tatgctgggt actgctgtcg gaaatcttcc cgaatgctat
      541 tcgtggtaag gcgctggcaa tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc
      601 ctggaccttc ccgatgatgg acaaaaactc ctggctggtg gcccatttcc acaacggttt
      661 ctcctactgg atttacgggt gtatgggcgt tctggcagca ctatttatgt ggaaatttgt
      721 cccggaaacc aaaggtaaaa cccttgagga gctggaagcc ctccgggaac cggaaacgaa
      781 gaaaacacaa caaactgcta cgctgtaatc ttcctgtcca gcacgccgcg ccagttcggc
      841 gtgctgtctt tttactcccg cttcagccgt ttcgaattac acagccacag agtgatcacc
      901 agtaacagga tcgcagccga gtagatcaac acatccagtg gcgatttatg atcgacgatg
      961 atcaagcgca caatggcggt gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa
     1021 ccggactgaa agtacttcac aatcagcgcg ataaattcga aatagagaaa gtaaaccacc
     1081 agctcatatt tgctggtttg ttctggcgcg aacagcacat ctgccagatg caccgtttct
     1141 ttgccgagga agacaaccaa aatcaggccg aggcacagca ggccaagatt gagtacggtc
     1201 tgcaaaatgg tggagataaa ctccacgcgc ggacgagaga gtgacgtcat agcagtacct
     1261 ccttcacagt ggcgagcttc ctgtataacg caaaaatgtg acggagatct atattttttg
     1321 tgtatgtttt gttcaacctg gatttggagc gcgcctccac caaagccgga tatacctacc
     1381 cgtaggccgg ataagccgcg cctggcggcg catccggcaa gacaaaccgc acccaagccg
     1441 gtaattcaaa aatcacccat tcaggtgatt cccagccacc agcaatgcgc gaaaaatcga
     1501 caatgttcct ctggaacaaa tgaaacgtaa cctgataaag gtggtaaaaa tgaagcctta
     1561 ttttgctgct ttgatgttat cagtctctgt tcttcctgct tatgctggtc cgctcggtac
     1621 tgctgataaa gccgacctac cgcaaagcaa tgtctccagc ccaatgatgg cccagtcgtt
     1681 caggcaacca gacctgcaac cgatatcgac ggacaggaag acagaatgtt ttcgccttta
     1741 cacaccagat cgcaaaccgg gggtgaactg cgttcctgat ggctcgacag gccattaaga
     1801 atgaaggtgc cagtgagtgc gaaaactggc acctttccca tgacttagcg gaaattgacg
     1861 ctacggtcgc tggtcatgtc atacaactgg aacttacgcc caagttgctg accaccgtca
     1921 cgcgtcagcg gcgtccagcc aatcgccgca cggctgcgtg tcgggccaga cgagaagaga
     1981 tccaacggta ccgagacata cacgcctttg gtgaagtccc cttcgccgta ctcctctttc
     2041 aaaacgttgg tgatcgtggc atagccaccc accaccacgc cgctgtcaaa gcgtttggcg
     2101 atctccagcg tgccgccttt atcccctgcc agatactgcc cgacgctggc tttcaccaac
     2161 acgtcctggg cgaaagatgg cgtccagtag gcggtcagat gtccggtttt cacgctgtag
     2221 tcggtgaatt tcatcatatc ttttgcacta cgccagtcgc gctgtttaac gtagttggca
     2281 tccagaccaa acgcccagtt actgtccagc ggacgataca acacttcggc ccccgcaccg
     2341 ccaaacatgg tttcgagata acctccatag acctgaccgt agaagccgtt gcccagatgc
     2401 tggaagtagt tggcttgcag gttattcaca tagacatcgt tctgcacata ctcgcgcaca
     2461 tgggtacgaa cgcgcggtaa gtgcgagtcc tgcggcggat tggtgtagtt aaacttgtcg
     2521 tagttgttgg caagatttgc aaacaggctg ccggtggtca gcagatggtc cgtcagccac
     2581 aaatctgcca ttcccatcac gcccagctga tacatgtaaa agttttccgg gccaccgacc
     2641 gactggttca gcaccggatc gatatggaaa tcaaagcgtg atttgtcgat ataccagccc
     2701 tgctcggtgg actgcggaac cactggctcg acgcgttttt gcgccagctt cgtttcgtgg
     2761 cccaacggct ctccggcgag atgatttttc aggctggcga catcggtttc cgtcgtcacc
     2821 tgcggcatgt taagacgatt ttccgtaatg cggatcgttt tgatcccatc cggcagatcg
     2881 ttcatcacga tccgattagc acggatgatc ccttcgcgcg aatcacgata tttcacctgc
     2941 tcgccggtga catacagcgt atcgcctttc gcctggatct gcggatcggc aagtccggca
     3001 ttgtatttca acagcgttaa ctgattcgcc accaccgaat gctgcaaaat ggcatcctgc
     3061 ggctgcggtt gatattgcgg gcgggcgtta tcgttgtaag acgggcgcag atcgttaaag
     3121 ttggtgcgca gcgtgacgcc aaacataaag gtgttgccac gttcatagct aaggttaacg
     3181 tcggcccaat cggtaacgcg ataaatcgca ccgacgttaa acttactttt ttgatccagc
     3241 ttcccggcaa aatcctgctg ataattattg ccttcatact ccagtttcag gcgcagcggt
     3301 tgccagggcg tctggtattc cacgccgcca aacagtgagg caggaccgtg gaacatctgg
     3361 ctaccgtcga tagatcccgc ctgtttgtag ctgttatcgc gatagcaata tttatcactg
     3421 gctgaacaga gcggattttt cacattaccg ctggtgccca gataacccca gcccaagccg
     3481 agcgtaaaat cgaacggccc ccaggctttg ctggcaacaa gatattccgc atcaaacagc
     3541 cccgtaccgc cgatatccct cgcaccaacc gccacttgcg gcagccagta actctcttcc
     3601 cacaaacgca gtttgagatc gaaggcttta tctttatacg tttgatcgcc agagaacgct
     3661 tcgacgctgc tgtactgccg ggtgcgcacg tcggtgtagc gcagcgttgt ttccagccac
     3721 gggaagagtt gcactgaagc tgagtaataa cggtactgat cgttatcgcg atagttcagg
     3781 ctcaactccc cttcccgcgc catgcgtgcg gtgggcgttt gtaataatcc tacgccaccg
     3841 aaatccgact gcgacgggcc aatgggcgca ggatatgttt cgccgtagca agccatgcta
     3901 atgcccagcg ccagtaagct aagcagatgt ctttttttca ttattggggt atccgctgcg
     3961 tcagagtctg aagaatgtcg gcgttaaggg catcaggcgt ctcactccag acggagtccg
     4021 cgaggccaac ataaataatg ctgccgggca tcggctctac gtgacgcttg ttccagtaag
     4081 ccaccggcgc tttttgcgtg cgtccgtccg ggtaaaccac ccacgcgtag ctgcgatccg
     4141 caccgctaag caggttctgc ccggagagat agctcgccac gtcgcgacct ggagtgaatg
     4201 gctgcttgcc aggacggctg ataagcccga acaacgtgac cgtggacggc ggtggtccga
     4261 cccacagcgt gtagttgccc tgcaacggtg ggttaccgcg ttcggcaacg cgcacgatat
     4321 cgggatcaag attgattttt tgcctgcccg tcactttcaa cgcctgaatt tgctggcgta
     4381 aggcgttaat ggcggcggca tcgtcggcgc tggagtctgc ggcctgttca gccagccgtg
     4441 tcagcaacgc ctgctgctga cgcaatgctg ccgccgttgc cagctcttcg ctaatcaccg
     4501 caccgggcca ccagctgttt gccagccgcg gttggcctac cagatcgatt aaatgctctg
     4561 cgcctgttag tgttttggcc tcactgctgc cattgctgaa caccttgacg gttcctgccg
     4621 cgaagaccga tgacgctccc acactcaaaa gcaacgcgac aaaagtttgt ttaatcattg
     4681 ttttgccgcc ttgatgagag tggttttcac cgggaaataa tccgcgccga gatactgttc
     4741 cgactggcga atttgccctt cgctgtcgat ccagtagcgg ttatgccagc gcgcctggtc
     4801 ggtggagact tcttcgtcca gcacgcgaac cagggtttca tcgctgccga ctttgacggt
     4861 atcggtgcca tcccatttga agactgagcg tgcggtggcg tagcgtacct gctggtactc
     4921 ggtccagccc atcgtgcgcg tccatgttgc accatcaaca atttgtgcgg gtttaatcag
     4981 cgggtcggcg gcaaggttat tcacttcaat caggttgtcg ccgccaagca aagtcttcac
     5041 cagacgacca tgttgtgtga caagagtggc ttgatcctga gtgacccatt tttgttgtcc
     5101 gtcttcagcg aaggccagca ccacaaacaa ttgcggcccg ccattaagct gcatgtactg
     5161 gctggcgtag ggcatatttt gaatatcatc gtccgtcagc tgtacgcctg gcgtgccgaa
     5221 cagactgtcc cacagtgaat tgcccagctc tttagtcgtg gctgaacagg cctgtaatag
     5281 caggcagata agaatgagtg caggtcgctt cacgactctt ctccagaggg gcagaataac
     5341 cacaccggag tgtggttatg gtaaatccct tcatacttca agccacagat gcaatgactc
     5401 aaattatttt ggggatacat actttattac tgggtactgg tggtcgtggt tgtggtggtc
     5461 ccggtattag aaccatcacc gccaccggtt gccgcgagcg cgacacccac cgccgagctt
     5521 acggtgctga cgctggtcgc ggaggaacta cccgccgaaa ccgacgttgc tgccgaccct
     5581 gccgcttcgc ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg
     5641 gcaaaaatgc catacagaac ttttttcata acaatttccc ttcattgaat gaatggagat
     5701 ttacccaaaa acatttcggg cgggatcgag tatacacaac taaagcatgc gaattacggc
     5761 gcggggaatt agcgatggcg ttttaggata attggaatga atatcaaata taagcttcaa
     5821 cttatattta aataacaata aaaatcataa atttaaaata aaaacaaaag taattaatct
     5881 gcgtattctc cgaaataaac aacgattaaa aatctatttt aggattaacc tcacggtatg
     5941 atttccgtta aatcacagac aaggggattt atctgataaa acaatgccga cttattgtcg
     6001 gcattgtttt taaggttatt taactcctca tactatgcat taatgtcgat attggcatca
     6061 ggctccggtt gccggatgcg gcgtaaacgc cttatccggc ctacatatcg gcatcaggca
     6121 cgcatgatga ttaaccgcgc cacgctttat agcggttaat cagaccattg gtcgagctat
     6181 cgtggctgct gatttcttta tcatctttca gctctggcag aatacggttc gccagttgtt
     6241 tacccaactc cacgccccac tggtcgaagg taaagatgtt caggatcacg ccctgagtaa
     6301 agattttgtg ctcatacagc gcaatcaacg cccccaggct gaacggagtg atttcgcgca
     6361 gcaggatgga gttggtcggg cggttacctt cgaacacttt gaacggcacc acgtagtcaa
     6421 gcgttgccgg atctttaccc tgatcgcgat attcctgctc aaccacttcg cgggatttac
     6481 caaacgccag cgcttcggtc tgggcgaaga agttagacag cagtttctgg tggtgatcag
     6541 agagcgggtt atgggtgata gccggggcga tgaaatcgca cggtaccatt ttggttccct
     6601 ggtggatcag ctggtagaac gcgtgctgac cgttagtgcc tggttcaccc cagataatcg
     6661 ggccagtctg gtaatccaca acgttaccgt tacggtcaac atacttaccg ttggactcca
     6721 tattgccctg ctggaagtac gccgcgaaac ggtgcatata ctggtcatac ggcagaatcg
     6781 cttcagtttc cgcaccaaag aaattgttgt accagatgcc aatcagcgcc agcaggacag
     6841 gcaggttttt ctcggcaggc gtggtggaga aatgcttgtc catcgcgtgt gcgccagaaa
     6901 gcagttcaac gaagttatca aagccgatgg agagaacaat cgacaggcca atcgctgacc
     6961 acaaagagta acggccgccg acccagtccc agaactcgaa catgttggca gtatcaatac
     7021 caaactcgcc aacggctttg gcattggtgg aaagcgccgc aaagtgtttt gcaacgtgtt
     7081 tttcatcacc tgccgctttc aggaaccagt cacgcgcgct atgggcgttg gtcatagttt
     7141 cctgagtggt gaaggtttta gatgctacca agaacagcgt ggtttccggg tttacttttt
     7201 tcagcacttc agcgatgtga gtcccatcga cgttagaaac aaagtgcatg ttcaggtgat
     7261 ttttgtacgg acgcagtgct tcggtcacca tgtacgggcc gaggtcagaa ccaccgatcc
     7321 cgatgttcac tacgtcagtg attgctttgc cggtataacc cttccactca ccggagataa
     7381 tcgcttccga gaaggttttc atcttctcca gcaccgcgtt gacttccggc attacgtctt
     7441 tgccatcaac cagaatcggg gtattgctac ggttacgcag cgctacgtgc agcacggcgc
     7501 ggttttcagt gcggttgatc ttctcgccag agaacatcga cttaatcgcg cccgccagat
     7561 cgcactcttt cgccagatcc tgtaatttcg ccagcgtctc ttcagtgatg cggtttttgg
     7621 agtaatccac cagcatctga tcgtcgaatg ttgcggagaa cttagaaaaa cgatcgccgt
     7681 ctttagcaaa aagatcggcg atcgtaacgt ctttcatttc atcgaagtgt ttctgtagtg
     7741 cctgccaggc agcggtctgc gttggattga tgtttttcat tagcaatact cttctgattt
     7801 tgagaattgt gactttggaa gattgtagcg ccagtcacag aaaaatgtga tggttttagt
     7861 gccgttagcg taatgttgag tgtaaaccct tagcgcagtg aagcatttat tagctgaact
     7921 actgaccgcc aggagtggat gaaaaatccg catgaccccg tcgttgacaa ccgccccgct
     7981 caccctttat ttataaatgt actacctgcg ctagcgcagg ccagaagagg cgcgttgccc
     8041 aagtaacggt gttggaggag ccagtcctgt gataacacct gagggggtgc atcgccgagg
     8101 tgattgaacg gctggccacg ttcatcatcg gctacagggg ctgaatcccc tgggttgtca
     8161 ccagaagcgt tcgcagtcgg gcgtttcgca agtggtggag cacttctggg tgaaaatagt
     8221 agcgaagtat cgctctgcgc ccacccgtct tccgctcttc ccttgtgcca aggctgaaaa
     8281 tggatcccct gacacgaggt agttatgtct gaaattgttg tctccaaatt tggcggtacc
     8341 agcgtagctg attttgacgc catgaaccgc agcgctgata ttgtgctttc tgatgccaac
     8401 gtgcgtttag tagtcctctc ggcttctgct ggtatcacta atctgctggt cgctttagcg
     8461 gaagggctgg aacctggcga gcgattcgaa aaactcgacg ctattcgcaa catccagttt
     8521 gccattctgg aacgtctgcg ttacccgaac gttatccgtg aagagattga acgtctgctg
     8581 gagaacatta ctgttctggc agaagcggcg gcgctggcaa cgtctccggc gctgacagat
     8641 gagctggtca gccacggcga gctgatgtcg accctgctgt ttgtcgaaat cctgcgcgaa
     8701 cgcgatgttc aggcacagtg gtttgatgta cgtaaagtga tgcgtaccaa cgaccgattt
     8761 ggtcgtgcag agccagatgt agccgcgctg gcggaactgg ccgcgctgca gctgctccca
     8821 cgcctcaatg acggcttagt gatcacccag ggatttatcg gtagcgaaaa taaaggtcgt
     8881 acaacgacgc ttggccgtgg aggcagcgat tatacggcag ccttgctggc ggaggcttta
     8941 cacgcatctc gtgttgatat ctggaccgac gtcccgggca tctacaccac cgatccacgc
     9001 gtagtttccg cagcaaaacg cattgatgaa atcgcgtttg ccgaagcggc agagatggca
     9061 acttttggtg caaaagtact gcatccggca acgttgctac ccgcagtacg cagcgatatc
     9121 ccggtctttg tcggctccag caaagaccca cgcgcaggtg gtacgctggt gtgcaataaa
     9181 actgaaaatc ctccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac tctgctcact
     9241 ttgcacagcc tgaatatgct gcattctcgc ggcttcctcg cggaagtttt cggcatcctc
     9301 gcgcggcata atatttcggt agacttaatc accacgtcag aagtgagcgt ggcattaacc
     9361 cttgatacca ccggttcaac ctccactggc gatacgttgc tgacgcaatc tctgctgatg
     9421 gagctttccg cactgtgccg ggtggaagta gaagaaggtc tggcgctggt cgcgttgatt
     9481 ggcaatgacc tgtcaaaagc ttgcggcgtt ggcaaagagg tattcggcgt actggaaccg
     9541 ttcaacattc gcatgatttg ttacggcgca tccagccata acctgtgctt cctggtgccc
     9601 ggcgaagatg ccgagcaggt ggtgcaaaaa ctgcatagta atttgtttga gtaaatactg
     9661 tatggcctgg aagctatatt tcgggccgta ttgattttct tgtcactatg cttatcaata
     9721 aacgagcctg tactctgtta accaacgtct ttatcgagaa taattgcctt taattttttt
     9781 atctgcatct ctaattaatt atcgaaagag ataaatagtt aagagaaggc aaaatgaata
     9841 ttatcagttc tgctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca
     9901 ccatcatggc actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac
     9961 ttaaaacact gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa
    10021 ccaacagcat taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga
    10081 aaaaagcccg ccaattgaag aaaaagcagg cttataaacc ggatcctgaa gcgtcattta
    10141 gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg aacaatcaac gcctgagatt
    10201 ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa ccctctcccc gaaggggcga
    10261 ggggactgtc cgagctcttt ttggacgttt tcatcagtct gaggcgttaa tcagcgtccc
    10321 ttctttttac gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt
    10381 tccgccgttt tttccatctt aacgactgga cgtttgatgc ccgctgtttt cggtttggcc
    10441 ttcgctttag gttttgcctc ggaagaggaa ttttcgatga gcttaaagag gtcgattaac
    10501 tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc cgcttaagct gacgttcata
    10561 atgcgcgtac gttccagctt tttcacttca tagccgaaat gctcgcacat gcgccggatc
    10621 tgacggttca gcccctgcac cagggtaatg cggaagacaa acggcgcttc ttttttgact
    10681 ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg cccccatacc acgaataaat
    10741 tcatcggtaa tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca
    10801 cggaggatct tattcaccag gtcgccgtga ttggtgagga aaatcagccc ctgggagtct
    10861 ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta
    10921 tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg gcttgttcag ggcgataagt
    10981 accaaatctt cggcttcccg aggctcaatc aactgaccgt ttactttcac aatgtcgccg
    11041 ggtttcacct gatcgccaat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg
    11101 atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa
    11161 cggactgatg agtcgggcag cataaattct cctgtaaaac gcggaatata ccgtacctct
    11221 tagcggggaa aaacagggtt atgcggcaac gtcgaaatta aatgtgatct gccacgcctt
    11281 ttatcacaaa agtgtctccg gtagtacgat gcaaatttgc ctggcaaaat aaggataatc
    11341 aacatattac gaatcgctgc acacctgtac tgatgactac tacgcagaga aacaaatgtg
    11401 caagagaaag cgctatatct ccccatgcaa agagtgaaaa atcgctttga aaggccctca
    11461 tgatggggtt atatcagatc aagcggtgag aaagcgtatt tgcacaaatg tgcagaacca
    11521 ggaggcggca atggaaaaca gtgacgatat ccgtttgatt gtgaagattg cccaactcta
    11581 ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt accgcaccac
    11641 catcagccgc ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg ccatcaacta
    11701 tgactacaac gaaaatctct ggctggagca gcaactgaag caaaagtttg gcctgaaaga
    11761 cgttgtggtg gtgtcgggaa atgatgagga tgaagagact caactggcga tgatggggtt
    11821 acacggcgcg caactgctgg atcgcttgct ggagcctggc gatattgtcg gtttttcctg
    11881 gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag gcggggcaat cgcggcagtt
    11941 aatctgcgta ccaattattg gcggaccgtc cggtaaactc gaaagccgct atcatgtaaa
    12001 cacattaacc tacagcgcgg cagcgaagct gaaaggggaa tcgcatctcg cggattttcc
    12061 ggctctgctg gataacccat taattcgtaa tgggatcatg cagtctcagc actttaaaac
    12121 catctctgcc tactgggata atctggatgt cgccctggtg ggaattggct caccggccat
    12181 tcgcgacggc gctaactggc atgcgtttta tggtggtgaa gagagtgacg acctgaatgc
    12241 ccgccaggtt gctggcgata tttgctcgcg cttttttgat attcacggcg caatggttga
    12301 aacgaatatg agcgaaaaaa cactctctat cgaaatgaat aaattaaagc aggcacgata
    12361 ttccattggc attgccatga gtgaagaaaa atacagcgga attgttggtg cactgcgtgg
    12421 aaaatatatt aattgtctgg taacgaatag cagcacagct gaactattac tgaaataaaa
    12481 atcttcccga tgtttagcgt aataaaaatt acggcggggc cctattatct aaaatcagga
    12541 gtctgttatg caaacgtggt taaatttgca ggataaaatc attattgtca ccggcggcgc
    12601 atccggtatt ggtctggcga ttgtagagga attattagca caaggcgcaa acgtacagat
    12661 ggtagatatt cacggtggcg atggtcaata tgaaggtcat aaaggctatc agttttggtc
    12721 gaccgatatt tccagcgcca aagaggtaaa tcatacggta gcggaaatta tccagcgttt
    12781 tggtcgcatc gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc tgctggtcga
    12841 tgagaaagcg cctgccgggc agtatgaact caacgaagct gcattcgaaa aaatggtcaa
    12901 tatcaaccag aaaggcgttt ttctgatgtc gcaggcggtg gcgcgacaga tggtcaaaca
    12961 acatgatggc gtgattgtga atgtttcctc agaaagtgga ctggaaggct cagaaggcca
    13021 aagctgttac gccgctacca aagccgcgct taatagcttc acgcgatcct ggtcgaaaga
    13081 attgggtaaa cacggtatcc gtgtggtcgg tatcgcgccg gggattctgg aaaaaacagg
    13141 actgcgcacg ccggaatatg aagaagcgct ggcgtggacg cgcaatatca ccgtcgagca
    13201 gctgcgtgaa ggctatacca aaaacgccat tcctattggg cgcgccggaa gattagcaga
    13261 agtcactgat tttgtttgtt atctgctgtc tgaacgcgcc agctatatca ccggagtaac
    13321 cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc tttatggtca atgccatttt
    13381 ttgcgcccac ggcaaattag cttgcgccat gctggaatcg gttcagatgg tgtacggcga
    13441 cgccagggtc gaggcagttg aatttgtgcc cggcgagaac gccggagaca tcgtagcaaa
    13501 gctgggaaag ttagtaagca ttcacaatca agatgagtgg ctgattgccg tggatttaca
    13561 gtgcggcagc ccgtggaatg cggcagcgat gcttgcgatg cgcaatcctc gtctgcgggt
    13621 aatcagcggc ctttctctgc cactggcgct cgaactggtt gataaccagg acagcatgaa
    13681 tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg ttgtctggaa
    13741 acaactggca acggcagagg aggatttctg atgaacatta cgctcgcccg cattgatgac
    13801 cgcctgatcc acggtcaggt cactaccgtc tggtcgaaag tggctaacgc ccagcgcatt
    13861 attatctgca atgacgaagt ttataacgat gaagttcgcc ggacattatt gcgtcaggct
    13921 gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc tgtttatcaa
    13981 aacccgcaat atcaggacga aaccgttttt tatcttttca ctcgcccaca ggatgcctta
    14041 gcgatggttc gccaaggggt aaaaatagac accctaaata ttggcggcat ggcctggcgg
    14101 cccggtaaaa aacagttaac caaagcggtt tcattagatg atgatgatat taacgcattt
    14161 catgagttaa ataatcttgg cgtcatactg gatttacgcg tcgtagcgtc agatccctca
    14221 atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa attaaataat agcgcttgtg
    14281 tacatatgct ttaacaggca gggatgacga cacttaaaag gtgaaaaatc atggaaataa
    14341 gtaccctaca aatcattgct atatttcttt tttcctgtat tgccggaatg ggcagcgtgc
    14401 tggatgaatt tcagactcat cgcccgttaa ttgcctgtac ggtgattggt ttaattctcg
    14461 gtgatttaaa aaccggaata atactcggtg gtacgctgga attgatagct ctcggctgga
    14521 tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc cagcataatc tccgccattc
    14581 tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg cctgtggctg
    14641 cggcaggcca ggtgctgact gtgtttgccc gtaccatcac cgtagtgttc cagcacgcgg
    14701 cggataaagc agcggaagaa gcacggtttc gcactctcga tattctgcat gtctccgcgt
    14761 ttggcgtgca ggcgctgcgc gttgctattc cggcactgat tgtctcactg ttcgtcagcg
    14821 ccgatatggt gagcaatatg ctgagcgcga taccggaatt cgtgacgcgc ggtctgcaaa
    14881 ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt gctacgtatg atgggcgtga
    14941 aatatttgat gcctttcttt ttcctcggtt tcctcgcagg tggctacctc gatctcagtc
    15001 tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt ctacatccag ctaaatccac
    15061 agtggcgtaa agctgaacct caactccagg ccaccgcctc caccgccctt gaccaacttg
    15121 acgattaacg gagccatcat ggaacagaga aaaattacac gcagcgatct ggtgagcatg
    15181 tttctgcgct ccaacctgca acaggcgtcc tttaactttg aacgtattca cgggctgggt
    15241 ttttgctacg acatgatccc cgccatcaaa cgactttacc cattaaaaga ggatcaggtt
    15301 gcggcgctca ggcgacacct ggtgttcttc aataccacgc cagccgtatg tggcccggtc
    15361 atcggcgtca ctgccgccat ggaagaggcg cgggccaacg gcgcggaaat tgatgacggt
    15421 accattaacg gcatcaaagt cggtctgatg ggaccattgg caggagttgg cgatccactg
    15481 gtctggggaa cgctgcgccc gattaccacc gcgctcggcg catctctggc actttcgggc
    15541 aacattctcg gcccgctgct gttcttcttt attttcaatg cggtgcgtct ggcgatgaag
    15601 tggtatggcc tacagctcgg ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg
    15661 aatgtgctgc aaaaactcac cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg
    15721 ctggtcacca aatggacgtc aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc
    15781 gatggctcca ccgtcaccat gaccgtgcag aacattctcg accaactttg ccctggtttg
    15841 ctggcgctcg gtctgacgct gcttatggtt cgtctgctca acaaaaaaat taacccggta
    15901 tggctgattt tcgccctgtt tggcttaggg attatcggta atgcgctggg cttcctgtcc
    15961 agattcttcg ccccggcacg actgccgggg ccatcgctca acatgaggtg gtttatgaaa
    16021 acaacagctc tgcgtcttta tggtaaacgt gatttacgcc tggaaacctt tgaccttcct
    16081 gaaatgcagg aggatgaaat cctcgcgacg gtggtcactg acagcctgtg cctctcttcc
    16141 tggaaagagg ccaatctggg tgaaaaccat aaaaaagtac ccgacgatgt ggcgaccaac
    16201 cccatcatca tcggccacga gttttgcggc gatattctgg ccgtgggtaa aaagtggcag
    16261 cacaaattcc agccgggtca gcgttatgtg attcaggcca acctgcaact ccccgaccgc
    16321 ccggactgcc ccggctactc cttcccgtgg gtgggcggcg aagccacgca tgtggttatt
    16381 cccaacgagg tcatggaaca agattgcctg ctggcatacg acggcgaaac ctattttgaa
    16441 ggctcactgg ttgaaccgct ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt
    16501 caggaaggta gttataacca cacgatgggg attcgcccgc aagggcgcat gctgatcctc
    16561 ggcggcaccg gaccaatggg actgttggcg attgattatg cgctacatgg acccgttaac
    16621 ccgtcgcagc tcgtcattac cgataccgac aacgataaat tgagttatgc gcgcaagcac
    16681 tatccatcag aaccgcaaac actgattcat tatctcaatg ccgccgatgc agcatttgat
    16741 acgctaatgg cgctgagtgg cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat
    16801 gaaggactgg tgactctcgc ctcttccttg ctggcgacag atggttgcct gaatttcttc
    16861 gccggaccgc aggataaaca ttccagcgcg ccaattaatt tctacgatgt gcattatgca
    16921 tttacccact acgtgggcac gtcaggtggc aataccgacg acatgcgcgc ggcggtcaaa
    16981 ctgattgaag agaaaaaagt gcaggccgca aaagtggtaa cacatattct tgggctgaat
    17041 gccgcgggcg aaaccacgct tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat
    17101 accgggaaat acctgccgct gacgtcactc acgcagattc aggatcaagc actggcggcg
    17161 attctggcgc gtcatcaggg gatctggtcc ggtgaggcgg agcaatatct gctcactcat
    17221 gcagaggcaa tttcccatga ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg
    17281 ccgcggcaac ttcgcgacct gatgtgcggc tgattgctga agcggcccaa tgtgcacgag
    17341 aaggataaaa aaacatcagc gccttaaggg gcgctcaaat tggtggcaaa gtacacattg
    17401 tttatgccgg atgcggaata aagccttatt cggcctacat aatctcgaaa attcaatata
    17461 ttgcagttat ctggtaggcc tggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc
    17521 agtctcagct cccggtgcaa tacgtgacat cgtgagccat gtcagtaagt ggttaaattc
    17581 gcagtggtag catgccttcc gtgatttctt actaaccgga gaagtaatgg aactgctttt
    17641 attgagtaac tcgacgctgc cgggtaaagc ctggctggaa catgcactgc cgctaattgc
    17701 tgaacagttg cagggtcgcc gctcagcggt gtttatccct ttcgctggcg taacgcagac
    17761 ctgggatgat tacacagcga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac
    17821 cggtattcat agcgttgtcg atcccgttgc cgcgattgaa aatgctgaga tcgtgattgt
    17881 cggcggcggg aatactttcc agttgctgaa acagtgccgc gagcgcgggc tgctggcacc
    17941 aattactgac gtggttaaac gtggtgcgct gtatattggc tggagcgcag gcgctaacct
    18001 tgcttgccca accattcgta ccaccaacga tatgccgatt gtcgatccgc aaggtttcga
    18061 tgcgctaaat ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg
    18121 ccataaaggt gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact
    18181 gacgattatt ggtctaccgg aaggtaactg gatcacagtg agtaaaggtc acgctacgct
    18241 gggtggcccg aacaccacct atgtgtttaa ggccggtgaa gaagcggttc cgctggaagc
    18301 tggtcaccgt ttttaagtga attgccggat gcatgatccg gcatcttgtt catagtgcct
    18361 gatgcgacgc ttgacgcgtc ttatcaggcc tacaggattc acaatattaa tactcgtccc
    18421 gtccttcatc ttcatccgac tgttccagca cactgtaagc caccgaacaa aacagcgagt
    18481 tcaggcgctg catatctccc agtaagccta aatgtagcga actggtttca atgctctgca
    18541 cgttttgctg atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa
    18601 agcgatgctt actacggcgc aaacgacgag cgctggtgac atcgccagag aagaacaccg
    18661 acattgccag ctttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaacc
    18721 catcaagcga aaatgcccgc cgtgctgcca gtgatttgtc agcaatttcg ctgcccatgc
    18781 gctcgacgat atcggaggcc tgttcaaggt tgagcgacat ttcaataatc tccgcccagc
    18841 ggcgcgactc ttcctccgcc agctcttctt ttggcatccg cgccagatac agcttaatgg
    18901 cggtgtagag aacgttgata tcatctgcca gcttacgcag ctctttctcc tgccgtggct
    18961 caccgtgcat cactttattc aacccttcca tcatctgttc catggcgtcg ccaatgcgca
    19021 gggtttcgcg cgcggcgttc gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca
    19081 gatgtttagg ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa
    19141 accgtgccat cgggtcaaca aatggcagca tgaccaggca acgtacaagg ttgtagaaga
    19201 cgtggaaata gatcaccagt tccgcttttg gcaatgacag cttccccatt gtctctgcca
    19261 gcaaatggac aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca
    19321 gcgcgacacg gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg
    19381 aaccgaggtt agcaccaatc accagacaga gcgccacggg gaaggagata atgcctgcgg
    19441 cggtcagagt cgcggtcagc agtacagcag caaggctgga gtagctgata atggcgaaca
    19501 tcgcgccaat cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa
    19561 cgccgtttgc ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagcaaaa
    19621 tcagcccaag accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga
    19681 ggaaaaaaat cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga
    19741 gaatacgcgc cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg
    19801 cgagggctac cagatcctga gcgacgaacg aggtcaccag catggtggtg gcattactgc
    19861 tctgtaccag tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc
    19921 tccggctaag gacagtacgc aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa
    19981 tatgagtccc ccagaccagc agggcgacgg cagaaagcag gtgaagcagc gttaacacga
    20041 gttcaggttc tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa
    20101 acacgctgac tggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt
    20161 ccagcctgat tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg
    20221 ccctgtttgt tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat
    20281 tcggtgccag ccagcgctca acatctgaga cgctcatacc tttacggcgg gcatagtctt
    20341 caacctgatc gcgctgaatt tgcgccacgg cgtagtactt gctgtccggg tggctgaaat
    20401 accaacccga aaccgatgca ccgggccaca tggcgaaaga ttctgtgagt ttcatgccgg
    20461 tatgtttttc cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg
    20521 ccgggtagcc cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc
    20581 tgaggttctc gttcggcgca tagccccagt agactttacg cacacgctca tgaagatact
    20641 ccgcaaaggc ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat
    20701 cgtgctgcgc ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa
    20761 atgcgccgat gtaatctgct ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt
    20821 agttagcgaa tcccgttttt tcggtctgtt gacgcagatg gtggctgaca ttgatcacat
    20881 gggtgcgcgt ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca
    20941 ggcccaccac gccacgcgga ttcagcgttt tctcggcgct taatttatcc agcatgtcgt
    21001 tggcgtcttt aaacagccgc tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgcg
    21061 gatacttccc ggccagcgac caggtcataa agaacggtgt ccagtcgatg taattacgca
    21121 gagtttcgat gctggcttca acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt
    21181 aagcctgcca gtcaaaagcg aagtcgttat cgcgcgccgc ttccagcgtg accggtggtg
    21241 tgcgcggttt cttgcgcccg tgctgaatac gtacggtttc gtactccttg cgggtacgag
    21301 cgacaaaatc atcgcgctga gtatcggaaa gcagcgccgc caccacacca acggtgcgcg
    21361 aggcattctg cacatacacc gtcgggccgc tgtagttctg ctcaattttc accgccgtgt
    21421 gcgcttttga ggtggtcgcg ccaccgatca gtaacggaat ggtgaagccc tgacgctcca
    21481 tctctttcgc cacgttaacc atctcgtcca gcgacggcgt gataagcccc gaaaggccaa
    21541 tcaaatcagc attcacttct ttagcagtac ggagaatttt ttccgcaggc accatcacgc
    21601 cgagatcgac aatttcgtag ttgttacatt gcagcaccac gccaacgata tttttaccga
    21661 tatcgtggac gtcgcccttc acggtggcga tcaccatctt gccgttggtt ttgccctgct
    21721 ctttgctggc ttcaataaac ggttcgagat acgccaccgc ctgtttcatg acgcgcgccg
    21781 ataaagtcct gcgcgtagtt gttgtgctct tcaataccgg tggcgaccgc gaagatgttc
    21841 gggtcgaaga tgatatcttc tggcgggaag ccaacctctt cggtgaggat tttgtacgcc
    21901 cgacggcaaa tctcgatttt ccgtgcgcga gtatcggcct gtccctgttc gtcaaaggcc
    21961 atcaccacca ctgccgcacc gtagcgacgc aacaatttcg cgtgatggat aaaggcgtcg
    22021 acgccctctt tcatcgagat tgagttaaca atgcctttgc cctgaataca cttcagccct
    22081 ttttcgataa cgtcccattt tgaggagtcg atcataatcg gcacgcgagc gatatccggt
    22141 tcaccggcaa tcagattgag aaaacgcacc atcgccgctt cggcatcgag catcccttca
    22201 tccatgttga tatcgataat ctgcgcgccg ttttccacct gctgacgcgc cacatcctgc
    22261 gcctcgctgt atttctcttc tttgatcagg cgcttgaact tagcagagcc ggtgacgttg
    22321 gtgcgttcac ccacgttcac aaacaggcta tcgtcgccaa tgttcagcgg ctccaggcca
    22381 gacaaacggc aggctacggg aatttccggc agtttgcgcg gcgctaatcc ttctactgca
    22441 cgactcatcg ctgcaatatg ttgcggcgtg gtcccgcagc aaccaccgac gatattgaga
    22501 aagcccgctt gcgcccattc acgtatctgt tttgccatcg tgtcggcgtc gagatcgtat
    22561 tcaccaaagg cgttaggcag cccggcgttc gggtgggcgg tgacgtagca ttccgcaatc
    22621 cgtgatagtt cctgcacgta ctggcgcagt tcatcgggcc ccagcgcaca gttcaggcca
    22681 aaggttagcg cttcggcgtg gcgcaatgag ttgtaaaatg cttcggtggt ctgcccggag
    22741 agcgtgcgcc cggaggcgtc ggtgatggtg ccggagatca taatcggcag ctcaacgccc
    22801 agcgcttcaa actccgtttt caccgcaaaa accgccgctt tggcgttaag ggtgtcgaaa
    22861 acggtttcaa tcaggatcag atccgcacca ccttccacca gcgctttggt ggattcgcga
    22921 taggccgcca ccagctggtc aaaagtgatg ttgcggaatg ccggatcgtt gacgtccgga
    22981 gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg caacgtagcg cggtttctcc
    23041 ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca gtttcgccgc cgcaaagttg
    23101 atttccgccg acagggattc catctggtaa tccgccatcg caatggtcgt ggagttgaag
    23161 gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg cgttgtggat agcggcgatc
    23221 acttccggtt tgctgagcac cagcaggtcg ttgttgcctt tgagatcgca tggccagtcg
    23281 gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat aactctggat catggtgccc
    23341 ataccgccgt ccagcaccag aatacgttca tttaactgcg cacgcagttg ttccactttg
    23401 ctgctcacac ttgctcccga cacgctcaac ccagatttaa taaaaattca acaaaccata
    23461 ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca acatgagatt tgttcaacat
    23521 taactcatcg gatcagttca gcaactattg cattagctaa caataaaaat gaaaatgatt
    23581 cccacgatac agaaaaagga gactgtcatg gtcgcaccca ttcccgcgaa acgcggcaga
    23641 aaacccgccg ttgccaccgc accagcgact ggacaggttc agtctttaac gcgtggcctg
    23701 aaattactgg agtggattgc cgaatccaat ggcagtgtgg cactcacgga actggcgcaa
    23761 caagccgggt tacccaattc cacgacccac cgcctgctaa ccacaatgca acagcagggt
    23821 ttcgtgcgtc aggttggcga actgggacac tgggcaatcg gcgcacatgc ctttatggtc
    23881 ggcagcagct ttctccagag tcgtaatttg ttagcaattg ttcaccctat cctgcgcaat
    23941 ctaatggaag agtctggcga aacggtcaat atggcggtgc ttgatcaaag cgatcacgaa
    24001 gcgattatta tcgaccaggt acagtgtacg catctgatgc gaatgtccgc gcctatcggc
    24061 ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct ttttagccca actgagcgaa
    24121 gaacaggtga cgaagctgct gcaccgcaaa gggttacatg cctataccca cgcaacgctg
    24181 gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc gcaaacgggg ttattcattt
    24241 gacgatgagg aacatgcgct ggggctacgt tgccttgcag cgtgtatttt cgatgagcac
    24301 cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt cacgtattac cgatgaccgc
    24361 gtgaccgagt ttggcgcaat ggtgattaaa gcggcgaagg aagtgacgct ggcgtacggt
    24421 ggaatgcgct gactttttct ggcgggcaga ggcaatattc tgcccatcat acctgagtgg
    24481 caatagaata agggtgtctg ttaatcgtat tgacgccaaa ataacttaat gttatacact
    24541 tcacacactt ttattaactc ggcattattt ttaaacatca aaccacttta attattaaca
    24601 aaatcgcaag atctcattcc gccgaaactt ttacggtttc attatgagat tgagc
//
LOCUS       DAJTNA010000045        24415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTNA010000045 DAJTNA010000000
VERSION     DAJTNA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24415
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.046"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(120..851)
                     /gene="rlmB"
                     /locus_tag="OO161_001537"
     CDS             complement(120..851)
                     /gene="rlmB"
                     /locus_tag="OO161_001537"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5462660.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1032..3473)
                     /gene="rnr"
                     /locus_tag="OO161_001538"
     CDS             complement(1032..3473)
                     /gene="rnr"
                     /locus_tag="OO161_001538"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5462661.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3512..3937)
                     /gene="nsrR"
                     /locus_tag="OO161_001539"
     CDS             complement(3512..3937)
                     /gene="nsrR"
                     /locus_tag="OO161_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5462662.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVSCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4142..5440)
                     /gene="purA"
                     /locus_tag="OO161_001540"
     CDS             complement(4142..5440)
                     /gene="purA"
                     /locus_tag="OO161_001540"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5462663.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5544..5741)
                     /locus_tag="OO161_001541"
     CDS             complement(5544..5741)
                     /locus_tag="OO161_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5462664.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5823..6827)
                     /gene="hflC"
                     /locus_tag="OO161_001542"
     CDS             complement(5823..6827)
                     /gene="hflC"
                     /locus_tag="OO161_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5462665.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6830..8089)
                     /gene="hflK"
                     /locus_tag="OO161_001543"
     CDS             complement(6830..8089)
                     /gene="hflK"
                     /locus_tag="OO161_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5462666.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8175..9455)
                     /gene="hflX"
                     /locus_tag="OO161_001544"
     CDS             complement(8175..9455)
                     /gene="hflX"
                     /locus_tag="OO161_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5462667.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9532..9840)
                     /gene="hfq"
                     /locus_tag="OO161_001545"
     CDS             complement(9532..9840)
                     /gene="hfq"
                     /locus_tag="OO161_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5462668.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9926..10876)
                     /gene="miaA"
                     /locus_tag="OO161_001546"
     CDS             complement(9926..10876)
                     /gene="miaA"
                     /locus_tag="OO161_001546"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5462669.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10869..12716)
                     /gene="mutL"
                     /locus_tag="OO161_001547"
     CDS             complement(10869..12716)
                     /gene="mutL"
                     /locus_tag="OO161_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5462670.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTSALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12726..14063)
                     /gene="amiB"
                     /locus_tag="OO161_001548"
     CDS             complement(12726..14063)
                     /gene="amiB"
                     /locus_tag="OO161_001548"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5462671.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14082..14543)
                     /gene="tsaE"
                     /locus_tag="OO161_001549"
     CDS             complement(14082..14543)
                     /gene="tsaE"
                     /locus_tag="OO161_001549"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5462672.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14515..16062)
                     /gene="nnr"
                     /locus_tag="OO161_001550"
     CDS             complement(14515..16062)
                     /gene="nnr"
                     /locus_tag="OO161_001550"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5462673.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDVLLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16061..17200
                     /gene="queG"
                     /locus_tag="OO161_001551"
     CDS             16061..17200
                     /gene="queG"
                     /locus_tag="OO161_001551"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5462674.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGVIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17183..17236)
                     /locus_tag="OO161_001552"
     CDS             complement(17183..17236)
                     /locus_tag="OO161_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462675.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17465..17540)
                     /locus_tag="OO161_001553"
     tRNA            complement(17465..17540)
                     /locus_tag="OO161_001553"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17505..17507),aa:Gly,seq:gcc)
     gene            complement(17577..17652)
                     /locus_tag="OO161_001554"
     tRNA            complement(17577..17652)
                     /locus_tag="OO161_001554"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17617..17619),aa:Gly,seq:gcc)
     gene            complement(17863..18408)
                     /gene="orn"
                     /locus_tag="OO161_001555"
     CDS             complement(17863..18408)
                     /gene="orn"
                     /locus_tag="OO161_001555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5462676.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18503..19555
                     /gene="rsgA"
                     /locus_tag="OO161_001556"
     CDS             18503..19555
                     /gene="rsgA"
                     /locus_tag="OO161_001556"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5462677.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19652..20620
                     /gene="psd"
                     /locus_tag="OO161_001557"
     CDS             19652..20620
                     /gene="psd"
                     /locus_tag="OO161_001557"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5462678.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREIIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20642..23965
                     /gene="mscM"
                     /locus_tag="OO161_001558"
     CDS             20642..23965
                     /gene="mscM"
                     /locus_tag="OO161_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5462679.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24116..>24415)
                     /locus_tag="OO161_001559"
     CDS             complement(24116..>24415)
                     /locus_tag="OO161_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5462680.1"
                     /translation="TLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVV
                     TFANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5604 a         6847 c         6219 g         5745 t
ORIGIN      
        1 tccatataga tttagtttaa atattaataa aatgaatatt tgcagtacgt aattatctta
       61 ccagctatag acaaaaaaaa accatccaaa tctggatggc ttttcataat tcagaggaac
      121 tagctgcgct gacgcaccgc ttcaaataag caaattccgg ttgcaaccga aacgttcaga
      181 gaagaaacac ttcctgccat tgggatgctg atcaactcat cgcaatgttc acgggtcagg
      241 cgacgcatac cttcaccttc cgcgcccatc accagcgcca ggcgtccggt cattttgctc
      301 tgatagagtg tatgatccgc ctcacctgcc gtaccgacga tccagatatt ctcttcctgc
      361 aacatacgca tggtacgcac caggttagtc acccgaatca gcggaacgct ttctgccgcg
      421 ccacaggcca cttttttcgc cgtggcgttg agctgggcgg agcgatcttt cggcacaatc
      481 accgcatgaa caccagcggc atctgcgcta cgcaggcacg cgccgaggtt atgcggatcg
      541 gttacaccgt cgaggatcag caggaacggc tgatcgagcg aagcgatcag atccggcaaa
      601 tcattttcct ggtactgacg tcctggcttc acgcgggcga taatgccctg atgcacggca
      661 ccgtcgcttt tctcgtcgag atattggcgg tttgccagct ggataaccac gccctgggct
      721 tcgagggcgt gaatcagcgg taacagacgt ttatcttcac ggccttttaa aataaagact
      781 tcctgaaaac gttcaggggc gcgctccagc agggcctgca ctgcgtggat gccgtaaatc
      841 atttcgctca ttaatgtact cgttgtttac gttttgcctg atgcactacg tttatccggc
      901 ctacatgatc tctgcaatat attgaatttg cgtgattatg taggccggat aaggcgttca
      961 cgccgcatcc ggcatgaaca aagcgcattt tgtcagcaat ctaaccctct tctgtttgaa
     1021 gagggtaatg atcactctgc cacttttttc ttcgccgcac gcttcgcttt ggtcgctgca
     1081 gctattttct gcgttttcgc cgatggcttt ttcgcttttc tcgcgtcttt cttcgctgct
     1141 ttcggcttcg tttttttctc accgcggaag gcgctgtctg gctcaaagtt taccttttta
     1201 ccgaccagac gacgcttgcc gcctttttta cctgcatcgc cttttttcgc tttctcgcgc
     1261 gccgttttac cgacgttgcg cggtgcgcgt tcgctggaga tcaggctaaa gtcgattttg
     1321 cgctcgtcca tattaaccgc ttcgacgcga acttccacgc gatcgcccag acgataagtc
     1381 tggccgctgg attcacccat cagccgttgc cctacctggt caaagcgata gtagtcattg
     1441 tccagcgaag agacatggac cagaccatca atgaataagt cgtccagacg gacaaagaat
     1501 ccaaagccag tgacgctgga aattacgcct ttaaagacgt tacctacctg gtcgagcatg
     1561 aagtcacact tcagccagtc agccacatcg cgcgttgctt cgtcggcacg acgttccgcc
     1621 atcgaacagt gctgacccag ttgcagcatt tcttccatcg aataatggta gccgccggtt
     1681 tcagtggtgt tgccctgatg cccctgctct ttcgccagca gatatttaat ggcgcggtgc
     1741 agcgtcaggt ctggataacg acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc
     1801 aggccaaagt gaccacggtt ttctggatcg taaatcgcct gtttcatcga gcgcagcagc
     1861 atggtttgca gcatttctgc atcaggacga tcggcaaccg actccagcag ctccgcgtag
     1921 tcacgcggtt ccggcttgtt accgcccggc agttccagcc ccagctccgc cagtactgaa
     1981 cggaaagagg taatcgcttc ggtgctcggc ttgtcgtgaa tacggaacaa tgctggttct
     2041 ttggctttct caacgaaacg cgccgccgag atattcgcca gaatcatgca ctcttcaatt
     2101 aacttgtgtg cgtcgttacg ctgagtctgt tcgatacgct caatacggca ttcagcgttg
     2161 aaaataaact tcgcttcttc gctctcaaat gagatcccgc cacgttcttc acgggcttta
     2221 tccagcactt tatagaggtt atgcaactct tcgagatgct taaccagcgg agcgtactgc
     2281 tcgcgcagat cctgatcgcc ctgcagaata tgccagactt tggtgtaggt cagacgcgcg
     2341 tgagagctca tcaccgcttc gtagaatttg tagcccgtca ggcggccttt cgacgaaacc
     2401 gtcatctcgc acaccataca caggcggtct acctgcggat tgagcgagca cagaccgtta
     2461 gagagcactt ccggcagcat cgggataacc tgcgaaggga agtacaccga cgtaccacga
     2521 ttacgcgctt ctctatccag cggcgttggc ggacgcacat agtagctgac gtcggcaatc
     2581 gcgacccata aacgccagcc gccgccgcgt tttttctcgc agtaaaccgc atcgtcaaag
     2641 tcacgggcgt cttcgccatc aatggtgacc agcggtaaat cacgcaaatc gacacggccc
     2701 gcttttgctt cttccggcac ttcttctttc agcccggcaa cctgttgctc aacagcctgc
     2761 ggccagatgt acggaatttc atgggtacgc aaagcgatat caaccgccat gccggtgccc
     2821 atattgtcgc ccagcacttc gacgatttta cccaccgctt tggtacggcg agtcgggcgt
     2881 tgggtcagtt caacaaccac cacaaagccc atccgcgcgc ccatgatctg atcgggcggg
     2941 attaagatat cgaagctcag acggctatcg tcaggaacca caaagccgac gcccgcttcg
     3001 gtaaagtagc gaccaacaat ctggctggtt tttggcacca gtacgcggac aatacgcgct
     3061 tcacgacgac ctttacggtc agcacccagc ggctgagcca gcacctgatc gccatgaatg
     3121 caggttttca tctgctcgct ggagagatac aaatcatctt tacgcccttc aacccgcaga
     3181 aagccgtagc catcacggtg gccaataacg gtacctttca ccaggtcgag gcgttccggc
     3241 agcgcatagc actgacgacg agtgaagacc agttgaccat cgcgctccat cgcgcgcagg
     3301 cggcgacgca ggccttcaag ctgctcttcg ccttcaatgt gcagttctac cgccagctca
     3361 tcacggctgg ccggtttttc acgtttggtt aaatgttcga ggataaattc ccgactaggg
     3421 atgggattcg cgtatttttc agcttcgcgt tcctggaaag gatcttgtga catctcggtt
     3481 cctccgttgt catctctgat gaagattttc gtcactccac cagcaataat ttataaagcg
     3541 gttgattctc ttcaaccaaa tcggcaagcg tgtagttatc cagttccgta agaaaacttt
     3601 gcacggcctt agaaagcgcc tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac
     3661 tgctgcaact caccagcgat aagggctcca actcgcgcac cacatcacca atacgtatcg
     3721 cactcgccgg tttacccagg cgaatgccgc cattttttcc acgaacagca gtcacgtagc
     3781 cggcacgact aagttgattg attattttga ccatatgatt acgggagacg ccgtagacgt
     3841 cagtcacttc agaaatactg gtcatccgcc cttctggcaa tgacgccatg tagatcagcg
     3901 cacgtaatcc gtaatcagtg aaactcgtta actgcacatc aacctcaaaa gggaaatcgg
     3961 gaaaaaatac atttatattg atgataaacc agccacaagc tgtgtcgcta atttatttca
     4021 gttcggggaa ggaaaaaagc gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc
     4081 gacacagcaa tgtatcacac tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga
     4141 attacgcgtc gaacgggtcg cgcagaatca tagtttcagt acgatccgga ccggtagaga
     4201 tgatatcgat cggcacaccg gtcagctctt caatacgctt gatgtagttc agcgccgcct
     4261 gcggcagccc gctacgatct ttcacgccga aagtggattc agaccagccc ggcatggttt
     4321 cgtaaatcgg ctctacacct ttccagtcgt cagctgccag cggagtggta gtcacttcgc
     4381 gaccatccgg catacggtaa gccacgcaga gtttaacctc tttcaggcca tccagaacgt
     4441 ccagtttagt caggcagaag ccagacaggg agttcagctg taccgcacga cgaacggcaa
     4501 cggtgtccag ccagccggta cgacgacgac gacccgtcgt tgcgccgaat tcgttaccct
     4561 gcttgcagag gaactcgcca gtttcatcaa acagttcggt cgggaaagga cctgcaccca
     4621 cacgagtgga gtaagctttg aggataccca gaacgtaatc aacataacgc gggcccaggc
     4681 cggaaccggt cgccacgcca ccagcagtgg tgttggaaga agttacgtac ggataagtac
     4741 cgtggtcgat atccagcagc gtaccctgcg caccttcaaa catgacgaaa tcgccacgct
     4801 gacgcgcctg gtcgagcagg tcagaaacgt caaccaccat agaagtcagg atgtcggcaa
     4861 cagccatcgt atcatccaga actttctggt aatcaaccgc ttcagctttg tagtagttaa
     4921 ccaactggaa gttgtgatat tccatcactt ctttcagttt ttcagcgaag gtttctttgt
     4981 cgaaaaggtc gccaacacgc agaccgcgac gtgccacttt atcttcataa gcaggcccga
     5041 taccacgacc ggtggtgccg atcgctttcg cgccacgcgc tttctcacgc gcgttatcca
     5101 gcgcaacgtg ataatcaagg atcagcggac atgcttcaga cagcagcaga cgctcacgaa
     5161 cggggatgcc acggtcttcc agttctttca tctctttcat cagcgcggcc ggagacagca
     5221 caacaccgtt accgatgatg ctggttacat tctcgcggag aatacctgat ggaataagat
     5281 ggagaacggt tttttcaccg ttgattacga gagtatggcc tgcgttgtga ccgccctggt
     5341 agcgtacaac atatttagcc cgttcagtca gaagatcgac gatcttacct ttaccttcgt
     5401 caccccattg ggtgcccagt acgacgacgt tgttacccat ttttcaaaat caccgtttgc
     5461 ttaaaaatgg attctaccat cgctttttca gagttacagc actttttgca ctcaaaaatg
     5521 acctcgatca gtctgttttt tgttcagcca atcgttttcc tcaacatgta gtagaccaca
     5581 acgcccgcaa ccacaagtcc accgccaaaa cgacgtaaaa tattatcggg caaattggtc
     5641 atcgcagaga tcatcttctt ccatgccttc gggtaaagca tcggccctaa accttccagt
     5701 accaaaacca gggcaagcgc cagccagatt gtcgaattca ttcattatcc ttatagaaaa
     5761 agaaaaccac cgacatccct gaggatgcgg tggctttatt gacctgtacc gcagtcgtta
     5821 tattaacgcg ttgcggaagt cggcgtcttc atgtagcgga agaaatcgct atccgggctc
     5881 atgaccatca cgtcctgatt gccagagaag ctgttctcat aagcacgcag gctacggatg
     5941 aatgcgtaga agtccggatc tttactgaat gcatcagcaa acagtttggc tgcttcggca
     6001 tcgccttcac cacgcatgat gcggccctga cgctcagctt ctgccagcgt tctggtcact
     6061 tcatagtcgg cagtcgcgcg cagtttttcc gcttcttcct gaccttgtga acggtgacga
     6121 cgcgctaccg cttcacgctc ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc
     6181 ggcaggttga tctgcttgat acgcacatcg acaacttcaa tacccagcgc cgccatactg
     6241 ttcgggttga tgaccggaac tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg
     6301 gcaatggcgt tatctgctgc cggggtagta acttcatctt ctgtacccgc agaaccggag
     6361 ttcagcgcgt cacgtacttc gagggtcaga cgaccacggg aatcagtgac gatatctttc
     6421 acgtccaggc gaccaatttc agaacgcaga cggtcagaga acttacgttt caacagcact
     6481 tccgcttgcg aaatgtcgcc accacccgtt gccaggtagt aacggctgaa atcgctgatg
     6541 cgccatttga tgtaagagtc gacgatcagg tctttcttct ctttggtcac aaagcggtcg
     6601 gcctggttgt ccatggtctg aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt
     6661 atcttgaaat gcagacccgg ctcataaacc agaggtttgt tgtcatcgtc acgcagtacc
     6721 ttaccaaaac gcagcgtaat accgcgctca ccttctttga cgacaaagac agacatgtaa
     6781 agcactacca gcacgatgat gataatcgcg ataactgact tacgcatcgt tattccccct
     6841 gacgctggta gtcgttacgc tgcgcgttgg cgcggcgttg gtccataata tcgccctgac
     6901 tggtggacga cgtgttgctt gctccactgg ttgtggagga agaggctggc ggcaaacgca
     6961 gcagattgct ggcaccgtta tcgctcttcg ccgcaggggc gttaccacct ttcagcatct
     7021 ggtctaacgg cagaaccatc aggttgccac ctttatcgtt aaccagcact ttgcgggtgt
     7081 tacccaacac tttttccatc gtctcgatat acagacgctc gcgagtaatt tccggcgcgg
     7141 ctttatattc cggcagaagt ttagcaaagc gcgccacttc accctgagct tccaggatgg
     7201 tctgggcctt gtacgcacgc gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg
     7261 gctgaacttc gttggtatac gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg
     7321 cggcaatcgc atcgtcaaac gccgctttta cttcttccgg cggacgagca gcctggaagt
     7381 tgacgtccag cagagtgatg cccatgtcat acggacgaat cgtctcttcc agttcgcgct
     7441 gggtatcgct acgaatcacg gtacgacctt ccgtcagaat gcggtccatg gtgtatttac
     7501 cgataacccc gcgcagggcg ctgtcggtag cctgacgcag gctgtcatcc gggctggtca
     7561 cgctatacag atatttttcc ggattggtaa cgcggtactg cacgttcatc tcaacgcgca
     7621 ctacgttctc gtccgacgtc agcatcacac cggaagcggc cagttcacgc acggcttcca
     7681 cgtttaccgg tttgacttcg tcgataaacg tcggtttcca gttcagaccc ggctcaacca
     7741 gatggctgaa tttaccaaag cgtgttacca cgccgcgttc ggcttcttta atggtataga
     7801 aaccactggc cgcccagata atgacaatcg ctgccgctgc gatggtaacg acacgaccgc
     7861 caagctgcgg gcgcgggcct tgcgatgaac tgccaccgcc agatccggtg cctttaccgc
     7921 cgcccagacc accgagcttt ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc
     7981 cttgatcgcg accgcctttg tttccatttc cctcagagtt gccgccaggt ttgctgcttc
     8041 cccacgggtc gcggtcttgt ccgttattac cgggctgatt ccacgccatg tttgtgctcc
     8101 atatttgtta tgcggtgatc cctgttgtct ttcgacgcca gaggatatga ctccacgctt
     8161 cagacgctac gccgttagat caggtaatcg atcaacgccg gttcttgttt acagaggcga
     8221 cgccagtcaa cgatcggcat acgaacttgc agacttacgc tgccgtcctc ctccatccac
     8281 tctttttcta ttgcctgaag ctgataaaaa cgacttctca gacgcccttc ctgcggtggc
     8341 agacgcaatg tatgctgcgc cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc
     8401 tgtggtatcc ccgctccggt ctgtgcggaa agccagacac ggatcggttt gttctcttcg
     8461 tcccgatcaa tacgcggttc gaaatcttcc agcatatcga tcttgttcat caccagcagg
     8521 gttgggatct cgtgagcgtc gatctcctca agaaccgtat tcaccgcttc gatgttttct
     8581 tgtacacgca catccgccgc atcaatgacg tgcagcagta atgtggcttg ccgcgtctct
     8641 tgtaacgtgg ctttaaatgc cgccaccaga tcgtgcggca gatggcgaat aaaccctacg
     8701 gtatctgcaa gtacggtttc accgacatcc gcgacgtcaa tacgccgcaa cgtcgggtcg
     8761 agggtggcaa acaactggtc tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg
     8821 gtagatttac cggcgttggt atatcccacc agcgaaacag taggaacgtc ggctttgata
     8881 cgcgattgcc gcccctgctc acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc
     8941 acgatgcgat tacgcaacaa acgacggtcg gtttcgagct gggtttcacc cggaccacgc
     9001 aaacctatcc cgcctttctg tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc
     9061 agatggcgca gctgcgccag ctcaacctgc aacttaccct catgggtacg cgcacgttgg
     9121 gcgaaaatat ctaaaataag gccggtgcgg tcgatgacac gacactcgca caaacgctcc
     9181 aggttacgct cttgcgccgg gctcagggca tggtcaaaaa gaacgaccga agcacccgtc
     9241 gctttgacag cttccgcaat ttcaactgct ttaccttcac ctacaaaata ctttgggtgc
     9301 ggcgctttac ggctaccggt aatcacctgc aatgcttcga caccggcgga agaaaccaga
     9361 gattcaaact cctggaggtc ttccatatct ttgtctttcg taaaatagat gtgtaccagt
     9421 accgcctgct caccagcatc ataacggtca aacaagcgta taaccctcta aatagatcag
     9481 cggggaacgc aggatcgctg gctccccgtg taaaaaaaac agcccgaaac cttattcggt
     9541 ttcttcgctg tcctgttgcg cggaagtatt ctgcgcgctg ctaccatgat ggtagttact
     9601 gctggtaccg ccaccggcgt tgttactgtg atgagaaacc ggacgagacg ggacaacagt
     9661 agaaatcgcg tgcttgtaaa ccatctggct gaccgtgttt ttcaacagga tcacgaactg
     9721 atcaaaagac tcgatttgcc cttgcagctt aataccattc accaaataaa tagaaactgg
     9781 aacacgttcc cgacgcagtg cgttcaggaa cggatcttgt aaagattgcc ccttagccat
     9841 tctctctttt ccttatatgc ttatttgtac tttgaacctt tcgattctga aaaaattgcg
     9901 cacgatacgt ctcaattgta cacattcagc ctgcgatagc accaacaacc tgtaatactt
     9961 cgtcacgcgc ctgttctggt ttttcactgt caagccagtg aaccccttcc caaccacgca
    10021 gccaggttat ctgccgcttc gccaactgtc tcgtggcgca aacacctcga taaaccattt
    10081 catcgtatga gatttcgcct tcaaggtaag accacatctg gcgataaccc acgcaacgaa
    10141 tggaaggcaa gtccgtatgc aaatctcctc gggcaaaaag cgcccggact tctgcttcaa
    10201 aacctgaagc caacatctga tgaaaacgct gctcaatgcg ttgatggagc agttcacggc
    10261 tcgccggggc gatggcgaac tgatgcacct gatacggtag agcgtctcct gacgtttgcg
    10321 tcagttccgt taaagtttta cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt
    10381 gtggatcatt tggatgaatc cttgctgccg caaccggatc tacctcctga agttgacgat
    10441 gcaatgactc ccaaccttgc tctgccgcct gttgctcaat tctggcccgc acttccgggt
    10501 ctgccgacgg tagcggcgac aacccttcca gcaatgcctt gaaatacaac atcgtaccgc
    10561 ccactaacag tgggatccgc cccgccgcgg tgatatcggc catttccgcc agcgcatcgc
    10621 ggcgaaaatc agcagccgag taagcctgcg acggatcgcg aatatccagc aatcggtgcg
    10681 gcgcggcgag taactcttca gcgttcggct tcgccgtccc gatatccatc cctttgtaaa
    10741 taagggcaga atcaacgctt atcaactcta ctggtaaaat tttacgcagc tcaatggcta
    10801 acgccgtttt accggaggcc gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct
    10861 tactgatatc actcatcttt cagggctttt atcgccggat gtaaatcaac agattgtaac
    10921 agaccacccg gcggcgtttt cacaagttgc ggacataacc gttccacgtc cgccagcagg
    10981 gttatggcct gtgccattga ccactgcgca tgttcgctca tcagatttcg tgcaatccac
    11041 tgcgcaatat tgccaggttc gaatacggac tgcttcgcca ggtagcctat cagttcagga
    11101 atcaagattt gtaaattttg ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc
    11161 tgtgcatctg actggaaatc aatacccaat tccgccaggg cagactgcgc tttttctaat
    11221 gccgattttt cttcgccaga aacttttagc cgcaacggaa taagcagcgg ctgagcgcaa
    11281 actggcgctt cacccggcgt cagttgtacc tgacgcagcc aacgttctgc cactggcaag
    11341 gctaaaagtg aaatgttgcc gtcgcgctcc agcaacgcac agtcggaatg gacgatagtc
    11401 agtacccgac caaaactctg actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct
    11461 tttaattttt gcatcggcgc gggcgtttgc aaaagctggc gatacacttc accttgctgt
    11521 ttctggtagc ctggctgcgc attcggccaa ggggcagccg gacgactgcc tgatgctggc
    11581 gcaggagtgt agcgcggagc taccggctca cgagctgccg gttctgcaaa gtgattgcgc
    11641 cccgccgcca cgcggttttc cggaatggaa cgcggtgcag gttggggttc atcgtccagc
    11701 ggtagcggcg tttccagttg ctgttgtagc acgctcagca cgccctgata gataaaatca
    11761 tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg cggggtgcac gttgacgtcc
    11821 acctgatgcg gatcgatctc cagatacagc acaaatgccg gttgctgatc ggcccccagt
    11881 ttgtcttcgc aggcctggcg gatcgcgtga ttgatcaggc gatcgcgcat catgcgaccg
    11941 ttcacgtagc aatactgaat ttctgccagt gcggacgtgg tgtgatttgg atcggccacc
    12001 cagccgcgta gcgtgagatc gccgtgttgc cattcaatcg ccagcgcttg ttcaagaaaa
    12061 gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc cgccttccgg cactgcgcgg
    12121 tactgacgca caattttacc gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc
    12181 gcaatgcggc ggatgatctc atcaatgtgg ttaaattcgg ttttctcggt gcgcaggaat
    12241 ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct ccagcgtcgt ccccacagga
    12301 tgcgccgccg gttttaccgt cacgttcata tcgcgccctt cggcataggc ctgccaggct
    12361 tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc
    12421 agcgcctcac cgcgaaagcc caggctgata atggcttcga gatcgtccag agaggcgatt
    12481 ttactggtgg catgacgagc cagcgccagc gccagctcat cttttttgat accgcagccg
    12541 ttatcacgaa tgcggataag tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc
    12601 gcacctgcat cgaggctgtt ttccaccagt tctttgacta ccgacgcagg tcgctcgacc
    12661 acctcacctg cggcaatctg gttcgccagt tgtggcggta agacctgaat tggcatcaat
    12721 cgtccttagt ttggcagcgt gcgatctggc gtcgtcaccg tactggccgt ttgtgccgtt
    12781 gcaccctgcg gcgcagattg catcggatgc gcaaggaaat aattgcgcag gcctttgtaa
    12841 atggcttctg ccagctgttg ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg
    12901 ttgctgataa aaccggtttc gaccagtact gacgggatat ccggcgaacg cagaacgccg
    12961 agactcgcgt gttctggtcg gcgtttatgt atttcgccaa tgcgttgcaa ctgactgatc
    13021 atactggtcg ctacatcata ccctacccgc tgggaatgac cgaactgtaa atccagcacc
    13081 gcctggctta aatagggatc agactgactg ttcgccagca catcgcccgc cccacccagc
    13141 agctccgaat gtttctcgtg ctgctccagc cagctggcca tttcgctgtt ggcgcgacgg
    13201 ttagagagca cccatacgga agcgccagtc gcactgcggt tcggtgcggc atcagcgtga
    13261 atcgacacga ggaaattagc gttttgctta cgtgccacat cgctgcgccc catcaccgaa
    13321 ataaagtaat ccccgtcacg ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa
    13381 gtacgcaatt tacgcgcgat ggcgatggtg acatttttct cccgcgtacc accggggccg
    13441 atagcgccag ggtcctgacc gccgtgtccg gcatcaatag cgatgataat tttatcgcca
    13501 gtgttagccg tcgcgcgcgc tgccggacgc gttaccgtat tactgctgat aacacccgta
    13561 gtgcggttac tttccgtttt aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca
    13621 accgcaggcg tttcaacgcg tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca
    13681 tcggcgttaa tcgtaaagac gacagtgtaa ttgctgccat tctgccgctt caccgcttcg
    13741 gttttaccat tttcggtaag atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt
    13801 ccagagcgaa tcgccttcac cagattattg ccgctgaaca acaacggcag tccctgaatc
    13861 acgcccgttt gtttgatatc gagcgccacg gtgcgtttgc tttgatggct aaacgcataa
    13921 tcaggatcgc caataaaact caacgttatc cgcgcctgtt gattaccgtt agaaacctga
    13981 atatcagaga gcgtcgcggc acccaccggc gtgcacagca gcagcagcgt cgctaccaac
    14041 caatttctga tgcgatacat catcccgcca cctttcaaag gttaaccggc taaacgcgcc
    14101 agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga
    14161 taatcaatgt gtatttcgac atccgggtca ggaagaacac ctgtaccttg ttgtggccac
    14221 tccaccaggc agatggcatc gttggcaaaa taatcgcgga tccccataaa ctccagctcc
    14281 tcgggatcgg caaggcggta caaatcaaag tgataaacca ttaaattgtc gagcgtatag
    14341 ggttcgacca gcgtataagt ggggcttttg acattaccct gatgacccag agcttgtaaa
    14401 aagccccggc taaaggtggt cttacctgcg cctaaatcgc catacagata gattacggtt
    14461 gcgccatcgc aggctttcgc tacccgctcg cccaggtcta atgttgcctg ctcatcaggg
    14521 agcggaatta ctcgattcat catggttttt atcagtcact tccgggttaa caatacgctg
    14581 tagcgtggaa aagagatcgg ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac
    14641 gtcagctgcc gcaccgtgcg cgacacagcc cgcacaggcg gcatcatacg gcgacagttt
    14701 ttgcccaagc aatgcgccaa taataccaga gagcacatcg cccatgccgc cgctcgccat
    14761 gcctgcattt ccggcatcaa taatgcctaa agcgtcagga tgggcggcga ccacggttcc
    14821 ggcacctttc agcaccgcta cgccgccata acgttgtacc agacgtttgg cgcaatgtaa
    14881 gcggtcactt tcaatttcag cgacggaaca gcctaacaac cgtgcggcct cgccaggatg
    14941 cggcgtgatc acgcgattgt gacgcttatc gggattgatt gccagcaggt tcaatgcatc
    15001 ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt tgcagtgctt ttttccccca
    15061 ctcttgctgg cccagaccgg gaccaatcac caccacatcg gcccattcca ggctttcggt
    15121 aagagagtcc atcgtcagtt catgcaccat caattccggt cgtgcagtca gcagcggcgc
    15181 aatgttttca ctgcgggtca gtactcggac taaaccagca ccagcacgca gcgccgcttc
    15241 ccccgtcata cgaatagccc ccgccgtgcc gtgatcgcca ccgataatta ccagccgccc
    15301 gtgatcgcct ttatgcgaag tcgggcgacg cggttttagc cagtgagaaa gttgttctgc
    15361 tgaaaaccgc tgaattttcg tctcctgacc tgccagccaa ctatccagcc ccagtgagtc
    15421 aaaatgcagt tgtccggtaa catcccgcgc ttttccagtg agcaagcctg gtttcagcgc
    15481 aataaaagtg atggtgtgat cggcgttgat caccgcgcct ggcgtagcgc cagtttcagc
    15541 cagcaggccg gaagggatat caaccgccac aatcggcgca ggatgggaat tagcgtggtc
    15601 gattaactgg ctaatggatt cgcggggcgc ttgccgcaaa ccggtaccga gcagcacatc
    15661 aacaatcaga tctaccgatt cgggccagac aatattcgaa gcatggatct cgccacccgc
    15721 gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc aacggtttgt cgctctcctg
    15781 ggcaaacaac gtgacctcaa tgccgaccgc tttggccagt cgcgcgacca cgtagccatc
    15841 gccgccgtta ttaccatgac cgcacagcac cagccagtgg cgggcgtcag gatacgccga
    15901 acgacacacc tggaatgcgg cctcgccagc gcgaagcatc agctcataga gtgtgagccc
    15961 cagcacatct gccgcctcgc gttctccgcg gcggatatcg tcggcgtacc agacggtgtg
    16021 tggtatactt acggggtttt tcttcattgt atggtccgtc atgtcagagc ccctcgatct
    16081 caatcagtta gcgcaaaaaa ttaaacagtg ggggctggaa ctgggctttc agcaggtagg
    16141 tattaccgat accgatctca gcgagtccga gcccaaactg caagcatggc tggacaaaca
    16201 ataccacggc gaaatggact ggatggcacg tcacggtatg ctgcgcgctc gccctcatga
    16261 gttattgccc ggtacgctgc gcgtgatcag cgtgcggatg aattaccttc ctgctaacgc
    16321 cgcatttgcc agcacgctga aaaaccccaa actcggctat gttagccgtt atgcgctggg
    16381 ccgtgactat cacaaacttc tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca
    16441 acattgtgtt tcgctgaatt ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc
    16501 gttagctgaa aaagctgggc tcggctggac aggtaagcac tcacttatcc tcaatcgcga
    16561 ggccggttcg ttcttctttt taggagaatt gctggtcgat attccgctgc ccgtggatca
    16621 accagtcgag gaaggatgcg gtaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc
    16681 catcgtcgag ccatataccg tcgatgctcg ccgctgtatc tcttatctca ccatcgaact
    16741 tgaaggggtg atcccggaag agttgcgacc gttaatggga aaccgtattt acggttgcga
    16801 tgactgccag cttatctgcc cgtggaatcg ctattcacaa ctcaccacag aagaggattt
    16861 cagcccgcgt aagccgctac acgcaccgga actcattgag ttattcgcct ggagcgaaga
    16921 gaagttttta aaagtcacgg aaggatcggc gattcgtcgt attggtcacc tgcgttggct
    16981 gcgtaatatc gccgtagcat taggcaatgc cccttgggat gaaacgattt tgacagcgct
    17041 ggaaagtcgt aaaggtgagc acccacttct cgatgagcac atagcgtggg cgattgcgca
    17101 gcaaatagag agacgaaatg cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct
    17161 ggttcgggtg attgaaaaag ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat
    17221 aaaaataaaa acgcattgca attcaagacg aataaaatcg tcaagtgatc gactcgacaa
    17281 aacggaatct taatttttaa cttatctatc aataggttaa aaaaatacta ttcaccgtgc
    17341 gaagtttctt tgaaattaag attacagcct gaggctgtgg ataactctgt ttacaagagt
    17401 ttgtcagaga caacgaaaac cagcaataac gcatcgctgt ggatatttta ttgagagaag
    17461 aatttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag gtcgtgctct
    17521 accaactgag ctattcccgc ttgggtggtc tgtgccttac agcacttttc aaattttgga
    17581 gcgggaaacg agactcgaac tcgcgacccc gaccttggca aggtcgtgct ctaccaactg
    17641 agctattccc gcgtactact taattttgct ttcgtaattt tcgcatttct gcgtcgttac
    17701 gggaggcgca ttatacgaga aatctttccc tctgcaaccc cccaaaaaag ttttttttgc
    17761 gtttttatct caagtgctga aattatcagc aagacgttca atttagcgtc aaaatcacgc
    17821 cgccgcaagt ggattccggc gcttacgtga ccaggaaaaa ttttacagct tgataaaatg
    17881 ctcgcggtag taagccagct ccgccaccga ttcacggata tcatccatcg cctgatgcgt
    17941 cccctgcttg gtaaaaccat ccagaatttc cggcttccag cggcgcgcca gctctttcag
    18001 ggtgctgaca tcgagataac ggtagtggaa gtaggcttcc agctccggca tgtatttaaa
    18061 caggaaacga cggtcctgtc cgatgctgtt accgcaaatc ggcgattttc ccgcaggcac
    18121 ccactgtttt aagaattcga gcgttgccag ttcagcttcc cgatcgccca tcgtgctcgc
    18181 tttcacgcgc tctaccagcc cgctggcggt atgggtgcgc acgttccagt catccatcag
    18241 cgccagctgt tcatcggact gatgtactgc aatggtcggc ccttctgcca gaatattcag
    18301 gttggcatcg gtcaccagcg tggcaatctc aataatgcga tcgcgctcgg gatccagacc
    18361 agtcatctca agatcgatcc aaatcaggtt gttttcattg gcactcatgc tattttccac
    18421 ccattcacag tgactatatt gatccacaat tgcgtgtatc atagatgttt tgcccatcag
    18481 gggcgaccag gagtctgtac gattgagtaa aaataaactc tccaaaggcc agcagcgccg
    18541 cgtgaacgcc aatcaccagc gtcgtcttaa aacgtctaag gagaagcccg actacgacga
    18601 caatctgttt ggcgagcctg atgaaggtat cgtcatcagc cgctttggta tgcacgctga
    18661 tgtggaatcc gccgatggcg acgttcaccg ctgcaatatt cgccgtacca tccgttcgct
    18721 ggtgaccggc gaccgtgtag tctggcgtcc gggtaaaccg gcggcggaag gcgtaaatgt
    18781 caaagggatc gtggaagcgg tgcatgagcg tacctcggtg ttgacgcgtc cggatttcta
    18841 cgacggcgta aaacctattg ccgccaacat cgaccagatt gtcattgtct ccgccatttt
    18901 gccggagctg tcactcaata ttatcgaccg ttacctggtg gcctgcgaaa ccttgcagat
    18961 tgagccgatt attgtgctca acaagataga cctgctggac gacgaaggca tggcgttcgt
    19021 caacgagcag atggatatct accgcaatat cggttatcgc gtattgatgg tttccagcca
    19081 tactcaggat gggctaaaac cgctggaaga ggcgttgacc gggcgcatca gcatttttgc
    19141 cgggcagtct ggcgtcggca aatccagcct gctgaatgcg ttgctggggc tgcaaaaaga
    19201 gatcctgacc aacgatgtct ctgacaactc ggggctcggt cagcacacca cgaccgccgc
    19261 tcggctgtat cacttcccgc acggcggtga tgtgattgat tcccctggtg tgcgtgagtt
    19321 cggcctctgg cacctggagc cggaacaaat cactcagggc tttgtcgaat tccatgacta
    19381 tttaggtctg tgtaaatatc gcgattgcaa acacgatacc gatccgggct gcgctatccg
    19441 ggaagcggtt gaggaaggga aaatcgcgga aacccgtttc gaaaactatc accgtattct
    19501 ggaaagcatg gcgcaggtaa aaacgcgtaa aaacttttct gatacggatg actgacaatt
    19561 aagctaaccg tcgttagaat cgtccccttt ttttcaggat ccggcatgtt ggccggatca
    19621 ggaacgacaa aacaatggcc tggaggctac cttgttaaat tcatttaaac tttcgctaca
    19681 gtacattctg ccgaaactat ggcttactcg cctggcgggt tggggcgcaa gcaagcgggc
    19741 aggatggctg acaaaactgg ttatcgatct gttcgttaaa tactacaagg tcgacatgaa
    19801 agaggcgcaa aagccggaca ccgccagcta ccgcaccttt aacgaattct ttgtccgtcc
    19861 gctgcgtgac gaagtacgcc caatcgatac cgatccgaat gtactggtca tgcctgccga
    19921 tggcgttatc agccagttgg gtaaaatcga agaagataaa atcctgcaag ccaaaggcca
    19981 caactacagc ctcgaagccc tgctggcagg caactatctg atggcggacc tgttccgcaa
    20041 cggtacgttt gtgaccactt acctctcccc gcgtgactac catcgcgtac acatgccgtg
    20101 caacggtatt ctgcgtgaga taatctacgt gccgggcgat ctcttctccg ttaaccatct
    20161 caccgctcag aacgtgccga atctgtttgc ccgtaacgaa cgcgtgattt gccttttcga
    20221 taccgaattt ggcccaatgg cgcagattct ggtcggagcg acgattgttg gcagcattga
    20281 gacggtctgg gcgggcacca ttacgccgcc gcgcgaaggt atcatcaagc gctggacctg
    20341 gcctgccggg gaaaacgacg gttctgtggc actgctgaaa ggccaggaaa tgggtcgctt
    20401 taaactcggt tccaccgtta tcaacctgtt tgcaccgggt aaagtgaatc tggttgagca
    20461 actggaaagc ctgtctgtca cgaaaattgg ccagccgctg gcagtatcta ccgaaacctt
    20521 tgttacgcca gacgctgaac ccgccccact tcctgctgaa gagatcgaag cagaacacga
    20581 cgccagccca ttggttgacg ataaaaaaga ccaggtctaa tccatcaaag gaaacgctga
    20641 cgtgcgcctg attatcactt ttctgatggc ctggtgcctc agttgggggg cgtacgccgc
    20701 gacggccccc gatagcaaac aaatcactca ggaactggag caggcaaaag cggcgaaacc
    20761 cgcacagccg gaagtcgtag aggcgctcca gtctgcctta aatgcgcttg aggaacgaaa
    20821 aggttccctt gagcgcatca aacaatatca gcaagttatc gataattatc cgaaactctc
    20881 cgctactctg cgcgcacaat taaacaacat gcgtgacgag ccgcgcagcg tgtcgccggg
    20941 aatgtctacc gacgcgctga atcaggaaat tctccaggtc agcagccagt tgctggataa
    21001 aagccgtcag gcccagcaag agcaggagcg cacccgcgag attgccgatt cgctgaatca
    21061 actgccgcaa cagcaaaccg acgcccgccg tcagttaaat gagatcgagc gccgcctggg
    21121 aacgcttacc ggcaatactc cgctcaatca ggcacaaaat ttcgcgttgc agtctgactc
    21181 tgcacgtctt aaggcgctcg ttgatgaact ggagctggcg cagctgtctg ccaataaccg
    21241 ccaggaatta gcgcgcttac gctcagagct ggcggaaaaa gagagccagc aactggatgc
    21301 gtatttgcag gccttgcgta atcaattaaa cagccaacgt cagcttgagg cggagcgggc
    21361 gctggaaagt accgaattgc tggcagaaaa cagcgccgat ttgccgaaag atatcgtcgc
    21421 gcaattcaaa attaaccgcg aactatcggc ggctttgaat caacaggcgc agcggatgga
    21481 tctcgttgcc tcgcaacagc gtcaggctgc cagccagacg ttacaggtcc ggcaggcgtt
    21541 gaatacgctg cgtgaacagt cgcaatggct gggatcgtcc aatctgctcg gcgaagcgct
    21601 gcgggcgcag gtggcacggc tgccagaaat gccaaaacca caacagcttg ataccgaaat
    21661 ggcgcagttg cgtgtgcaac ggttacgtta tgaggatctg cttaataaac agccgctgct
    21721 acggcaaatc catcaggccg acggtcagcc gctgaccgcc gagcaaaacc gtattctgga
    21781 agcacaactg cgcactcagc gtgagttgct gaactcattg ttgcagggtg gcgacacgct
    21841 actattggaa ctgaccaagc tgaaagtctc caacgggcaa ctggaggatg cgctgaaaga
    21901 ggtgaacgaa gcaacgcacc gctatctgtt ctggacctct gacgtgcgcc cgatgaccat
    21961 cgcctggccg ctggaaatcg cccaggatct gcgtcgtctc atttcgctgg acaccttcag
    22021 tcagttgggc aaagccagtg tgatgatgct gaccagcaaa gagacaattt tgccgctgtt
    22081 tggcgcgttg attctggtcg gttgcagtat ttactcgcgc cgctatttca cccgttttct
    22141 tgaacgttcg gcggcgaaag ttggcaaagt gactcaggat cacttctggc tgacgttgcg
    22201 cactcttttc tggtcgattc tcgtcgcgtc accgttaccg gtgctgtgga tgacgctggg
    22261 ttacggcttg cgcgaggcgt ggccttatcc gctggcggtc gcgattggcg atggtgtaac
    22321 ggctaccgtg ccgctgctgt gggtagtgat gatttgcgcc acctttgccc gcccgaacgg
    22381 cttgtttatc gctcattttg gctggccgcg cgaacgtgtt tcccgtggga tgcgctacta
    22441 cctgatgagc atcgggctta ttgtgccgct gattatggcg ctgatgatgt tcgataacct
    22501 cgacgaccgt gaattctccg gttcgctggg acggctttgc tttatcctca tttgcggtgc
    22561 gctggcggtg gtcaccctca gcctgaaaaa ggccgggatc ccactgtatc tcaacaaaga
    22621 gggcagcggc gacaacatta ccaaccatat gctgtggaac atgatgattg gcgcgccgtt
    22681 ggttgccatt ctggcgtcgg ctgtgggtta tctggcaacg gcacaggcgc tgttagcgag
    22741 gcttgaaacc tcggttgcca tctggttcct gctactggtg gtttatcacg ttatccgccg
    22801 ctggatgctg atccagcgcc gcaggctggc gtttgaccgg gcgaagcatc gccgggcaga
    22861 gatgttagcg caacgtgcgc gtggcgaaga ggaagcgcat catcacagta gcccggaagg
    22921 agcaattgaa gtcgatgaaa gcgaagtcga tctcgatgcc atcagtgcgc aatccttgcg
    22981 gctggtgcgc tcaattttga tgttgatcgc cctgctttct gtcattgtgc tgtggtcaga
    23041 aatccattcc gctttcggct tcctcgaaaa tatttcgctg tgggatgtca cctccacggt
    23101 acaaggcgta gaaagtctgg agccaattac cctcggtgcg gtgctgattg ccattctggt
    23161 gtttatcatc accacgcagc tggtgcgcaa cctgcccgcg ctgctggaac tggcgatttt
    23221 gcagcacctg gatttaacgc cgggtacagg ctacgccatc accaccatca ccaaatatct
    23281 gctgatgctg attggcgggc tggtcggctt ctcgatgatt ggtattgagt ggtcgaaatt
    23341 gcagtggctg gtcgccgcgc tcggtgttgg tctcggtttt ggtttgcagg aaattttcgc
    23401 caactttatc tctggtctga ttatcctgtt cgaaaaaccg attcgcattg gcgatacggt
    23461 gacaattcgc gatctcaccg gtagcgtgac gaaaattaac acccgcgcca ccaccatcag
    23521 cgactgggac cgtaaagaga taatcgtgcc gaacaaggcg tttattaccg agcagtttat
    23581 caactggtcg ctctctgact cggttacgcg cgtagtgttg acgattccgg cccctgctga
    23641 tgccaacagt gaagaagtga cggaaatcct gctcaccgca gcgcgtcgct gctcgctggt
    23701 gatcgacaac ccggcaccgg aagtcttcct ggtggatctg caacaaggga ttcagatttt
    23761 cgagctgcgt atttatgccg ctgagatggg tcaccgtatg ccgctacgcc atgagatcca
    23821 ccagctgatt ctggctggct tccatgccca cggtatcgat atgccattcc cgcccttcca
    23881 gatgcgtctg gaaagcctca acggtaaaca aacggggaga acgctgacgt ctgcgggcaa
    23941 aggtcgtcag gcgggaagtt tgtaatgcat gaagctgcca aatgatgtca acgtggctca
    24001 tttagcagct taacatcatc ttcagttatc accggccctg gagagtatca tccatacgca
    24061 ttacttcctg acaactcatc gccggagaga cgatgagttg caggatttca tatgatcagt
    24121 cgagagctaa ctcaggttta ttcgccatgc gactgcaata gttctggtaa atcgccatcg
    24181 ctaacagcga gaagaagaca gggccgccaa tcatccacaa tgtgctgtcc cagtctccgg
    24241 cttcaaccac aggcggaata atggtgaaga cgttcgcaaa tgtcaccacc agtacgacaa
    24301 ccaccgtcgc aatcattgcc gacatacgcg ttttgaaaat cacaaatggt ctgtcgagat
    24361 cctgacgtgc tttaaagaat gggaaagcca gcgcgaggaa caggtaagga agcgt
//
LOCUS       DAJTNA010000046        24171 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTNA010000046 DAJTNA010000000
VERSION     DAJTNA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24171
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.047"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(132..1142)
                     /locus_tag="OO161_001560"
     CDS             complement(132..1142)
                     /locus_tag="OO161_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR5462681.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVASFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(1175..1999)
                     /locus_tag="OO161_001561"
     CDS             complement(1175..1999)
                     /locus_tag="OO161_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5462682.1"
                     /translation="MLAVDQREAMRLMFAAAGQPKPIADSVLTDFKVAATRILSPYAS
                     AVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNGIPVDSVEIDMSVDPHKAREMG
                     AKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVSIIEPVVRPPRRGWDFDRESAI
                     VAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQLNDQMKMPWVILSSGVDADIFG
                     RAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLCDVSVPRLQRLAEIVDEGIAQR"
     gene            complement(2087..2965)
                     /gene="yihU"
                     /locus_tag="OO161_001562"
     CDS             complement(2087..2965)
                     /gene="yihU"
                     /locus_tag="OO161_001562"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5462683.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            3138..4034
                     /locus_tag="OO161_001563"
     CDS             3138..4034
                     /locus_tag="OO161_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5462684.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            4074..4862
                     /locus_tag="OO161_001564"
     CDS             4074..4862
                     /locus_tag="OO161_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5462685.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(4968..6791)
                     /gene="typA"
                     /locus_tag="OO161_001565"
     CDS             complement(4968..6791)
                     /gene="typA"
                     /locus_tag="OO161_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5462686.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYSSALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
     gene            7164..8573
                     /gene="glnA"
                     /locus_tag="OO161_001566"
     CDS             7164..8573
                     /gene="glnA"
                     /locus_tag="OO161_001566"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5462687.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            8747..9796
                     /gene="glnL"
                     /locus_tag="OO161_001567"
     CDS             8747..9796
                     /gene="glnL"
                     /locus_tag="OO161_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5462688.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGRESGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            9808..11217
                     /gene="glnG"
                     /locus_tag="OO161_001568"
     CDS             9808..11217
                     /gene="glnG"
                     /locus_tag="OO161_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5462689.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            11328..11438
                     /locus_tag="OO161_001569"
     CDS             11328..11438
                     /locus_tag="OO161_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5462690.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(11628..13001)
                     /gene="hemN"
                     /locus_tag="OO161_001570"
     CDS             complement(11628..13001)
                     /gene="hemN"
                     /locus_tag="OO161_001570"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5462691.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VECYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(13190..13699)
                     /gene="yihI"
                     /locus_tag="OO161_001571"
     CDS             complement(13190..13699)
                     /gene="yihI"
                     /locus_tag="OO161_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5462692.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            14281..14913
                     /locus_tag="OO161_001572"
     CDS             14281..14913
                     /locus_tag="OO161_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5462693.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(15295..18081)
                     /gene="polA"
                     /locus_tag="OO161_001573"
     CDS             complement(15295..18081)
                     /gene="polA"
                     /locus_tag="OO161_001573"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5462694.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            18445..19377
                     /locus_tag="OO161_001574"
     CDS             18445..19377
                     /locus_tag="OO161_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5462695.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MAINVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(19418..20848)
                     /locus_tag="OO161_001575"
     CDS             complement(19418..20848)
                     /locus_tag="OO161_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5462696.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIYPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(21003..21629)
                     /gene="dsbA"
                     /locus_tag="OO161_001576"
     CDS             complement(21003..21629)
                     /gene="dsbA"
                     /locus_tag="OO161_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5462697.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(21646..22632)
                     /gene="srkA"
                     /locus_tag="OO161_001577"
     CDS             complement(21646..22632)
                     /gene="srkA"
                     /locus_tag="OO161_001577"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5462698.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDLRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(22709..22978)
                     /locus_tag="OO161_001578"
     CDS             complement(22709..22978)
                     /locus_tag="OO161_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5462699.1"
                     /translation="MKCKRLNEVIEFLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            23048..23632
                     /gene="mobA"
                     /locus_tag="OO161_001579"
     CDS             23048..23632
                     /gene="mobA"
                     /locus_tag="OO161_001579"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5462700.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            23614..24141
                     /gene="mobB"
                     /locus_tag="OO161_001580"
     CDS             23614..24141
                     /gene="mobB"
                     /locus_tag="OO161_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5462701.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5914 a         6143 c         5893 g         6221 t
ORIGIN      
        1 atatcaatta cgtcctgtgg ccgactaatt gaataagttg caggactggt gatttgactc
       61 atcgcagtgt cctttttaat tgttgtaggg tggcgctgac gtgacgcagc gccagacaga
      121 gatttagaaa atcattccgg cgcaaattgc agcatgacct ttatggagtc attttgctgc
      181 gcgcgttcaa atgccgccgc tgcatcctca attgcaaata aatcggtaac cagttcactg
      241 gtatcaaacc gcccatccgc cagccattcg ataacagcat cgaaatcttc agggatgtac
      301 atgccagagt tgagcaagtc acgctcgaaa cgttgcatca tcggtaatgg tatttcacgc
      361 ggcccacttg gtacgcccat gcacacgacg gtgcctccgg catatacacg ggtacatgca
      421 tcgttaagcg tggcctgcgc tgcaacgaca tcaatcgccc ccgtaaatcg cacatctgcg
      481 gccagctcac ctggtgccca aacttctgct gcgcccagct ttaacgccag cgcgcgtttg
      541 gcagcgtcag gttcaatcac ggtaatctta cctgccccca taatgcgcag tgcctgcacg
      601 atggataagc ctatcgttcc ggcaccaatt accagtacgc tttccagaga agctttcggc
      661 attcggttaa cgcagtgacg ggcacaagcc gccggttcgg caaaggccaa cacttttagc
      721 ggtaaagagg ctggtgcgac atggcaatta cgcgcaggaa caatgtgttg tgagcgagca
      781 aagcccggtg cgcgaaaact agcaacctgc ggtggttcac aaagattaaa acgtcctgct
      841 ttgcaggctc ggcattccat gcaagccatg atgggatcga ccacaacatg atcgcccggc
      901 tgtacatttt taacgtcgct gccaacttcc gtaacgcgcg ctgcaatttc atgaccgggg
      961 acgacaggtg gtttggcaaa cggatgaccg cctttcagaa catgcagatc cgaaccgcat
     1021 atcccataga acacaggcgc aatccgcact tcgtgcggtt taagttcctg gtgtggtact
     1081 tcaacatatt gagtggtgac tttcccaata tctgaaaata aaacctgggt aattttatcc
     1141 attgttattc tccagaatcg atatccatag gggattagcg ttgcgcgata ccttcatcga
     1201 caatttctgc caaccgttgt aaacgaggaa cagagacatc gcacagcata gtttgtggat
     1261 cttgtgcacc aacaaccgac gcccaaaccg ctcgtccagc caggaagccc gacgcaccac
     1321 ctttcatggc aatacttact gcacggccaa aaatatcggc atcaacgcca gaagaaagaa
     1381 ttacccacgg catcttcatc tgatcgttaa gttgctgaca ggcggcaagt aaggtttttt
     1441 catcgccttt gccccctaat ggcatctcgg ctttgtaaag atcagcctct gttccaccaa
     1501 gctctgcggc ggcggcaaca atggcgcttt cacgatcaaa gtcccaaccg cgacgaggtg
     1561 gacgcaccac aggttcaata atgctcacca gcccggcact acggcagcga cgaacaaatt
     1621 tatcaaccat tgccagacgt tcctctgcgg gttcatcttc acgccagagt acaagtaatt
     1681 tcattgcctt agcccccatt tcacgggctt tatgcgggtc gacggacata tctatttcaa
     1741 cgctatcaac cgggatgcca ttacctggga taaaaagatc ggcagcgaca atcaggccgc
     1801 aagaattagc aacagcgccc tgctcgacta tttgctcaag acaaaattgt ttatccgcca
     1861 gaacggcaga agcataagga gaaaggattc gggtcgcggc aactttaaaa tcggtgagta
     1921 cactgtcggc gatgggtttt ggctgccctg ctgccgcaaa catcaagcgc atggcctcac
     1981 gctgatcgac agctaacatg gcaaatccac cacctgggcg ggagatatct ttcagcgtaa
     2041 atggggtgaa gtgggattac atagaaactc ctgttaatag ataaaattaa agaccagcct
     2101 gctggcgaac ctgatttagc agggcactcc agtcctgccg ccctttgccg tcttgacttg
     2161 ctgcagcgta ataactttct gctgcaatcc ccatattgag cggtgccccc gtctcccgag
     2221 cgacgtcaac tgcaatacgc agatctttca acgccagatc caccatgaag gcgggtgaaa
     2281 gatcgccttt cagcactttg ccaggccagg tggtcgtaaa atgtcctttc cctgctgccg
     2341 taccactcat caccttaatt gcgacatcaa gatttaatcc cagtgactca cataacacag
     2401 cggcctcgga ggacagcgca ttaagcgcaa tgctcatata gttgttaatt agcttcacgc
     2461 ggatccccat tcctgggccg ccagcctcga ccagctcatt acccatgcac ataagaatcg
     2521 ggcgtgcacg tgcaatctgc tcatcagtgc cacccgctaa aatcagcaat gtgccatcaa
     2581 tcgcattcac ggacgttctg cccacgggag cgtccatcat actgatgcct ttttcttgta
     2641 actcccgtat taatgcatcg gtctgtaagg gatgaatagt cgacatatca atcaataacg
     2701 cgtcagatga aacagcctca cagacgcctt tttcccccaa cagaacctgt cgaacaatgt
     2761 caccattggg aagcatagtg atgataaatt cagcgcctgc tgctgcttcc gctggggttt
     2821 gtgccgcagt ggccccctgc gtaactagcg catcgacggc ctgtgcattg acatcaaata
     2881 cctgcaatga atgccctttt tgcaatagat tgttagccat tgggctaccc atctgcccca
     2941 gcccgagaaa tgcaatggtt gccatgaata ctctcctgtc tgacattgac aaaataatga
     3001 caattgaaaa ttcgttttcg tctgtttttg agcatatttg taatttatgt tcaaaaacac
     3061 gacacgcatc actaatttga tcatttgcgt catttaaatt gaccaaagac gacaaaatta
     3121 aataggacaa gacaaatatg accagaattg catgcgtggg tatcacagtt caggacagaa
     3181 tttattcttt gcccactctg ccggagggcg gcggaaaata tcaggcaaat cattatcttg
     3241 agattggtgg cggcccggca gcaacagccg ctgttgcaat cgcaaagctt ggcgtggagg
     3301 ttgatttcat cggtcgcgtc ggcgatgaca gttgtggaaa taccttactt gcagaacttg
     3361 agggttgggg cgtcaatacc gcattttgcc gccgatatcc taacgcccgt tcgtcgcaat
     3421 cagcgatcct cgttgatcag catggcgaac ggattatcgt taattatcca agtcctgatc
     3481 tcggtactga tgccgaatgg ttagaagcga tcgatttctc tcgttatgat ctgattctgg
     3541 ccgatgttcg ctggcatagc ggtactgaaa aagcattctc gctagcgcgc cttgcgggtg
     3601 tgacaacgct tttagacgcc gatatgacac cgcaggatat ttctccactt gtcgcgcttg
     3661 ccgatcacgc cgtgttctca acaccagggc tgaaacgcat gactggcctg cagtcacctg
     3721 aagaaggttt gttccaggca acgacacaaa cagcaggtaa agtgtatgtt acgctgggtt
     3781 ctgaaggatc gttgtggatt gaagatggtc atttgtgtca acaggaggca ttttctgtca
     3841 atgtggttga taccactgga gcaggcgatg tttttcacgg cgcactcgcg gtagcgttgg
     3901 cagaaaaaat gccaacaaaa gaggctattc gttttgccag tgcggttgcc gcgatgaaat
     3961 gtacccagcc tggcggtcgg gccggcattc ctaatcgtga gcaaaccgaa tcatttttgt
     4021 cactttatgc gtaaaatggt aaacatctac cgaggagggg caagggagta ttcatgagta
     4081 tcatcgaagt gacaggtaat ccacgccatg accaactcgt tcatctaatc gcagaacgcg
     4141 gttatatgaa tattgaagaa ctggcacaac tgctggatgt ttcaacgcaa accgtgcgcc
     4201 gggatatccg aaaactcagt gagcagggct tgatcacccg tcatcatgga ggggccgggc
     4261 gcgtctccag cgtcatgaat accgcttttg agcaacggga actttcgctc accgccgaga
     4321 aacgggcgat cgctgaggca gtcgccgatt accttcccga acgctgtacc gtctttatca
     4381 ccatcggtac aaccgtagaa gccgttgcca gggcattact caaccggcgt gatttacgca
     4441 ttatcactaa cagcctacgt gtggcacaga ttctttataa gaaccaggat attgaagtga
     4501 tggtgccggg aggcacttta cgcgctcata acggcgggat tatcggccca ggagccgtgg
     4561 attttattga aggcttccgc gcagattatt taatcaccag tataggtgcc attgaacatg
     4621 acggcaccct actggaattt gatgtaaatg aagcgttagt cgcgagaacg atgattaaac
     4681 atgcgcggaa tacattgtta gtggccgatc atacgaagtt tgccgcgtct gctgccgttt
     4741 caattggcaa tgcacggaat gtcagggctt tttttactga tgccccgcct cccaattctt
     4801 tctgccagtt gttaagtgaa gagaatgttg aactggtggt tgccgagcaa gaagtatcct
     4861 gaaattgaaa tttcggaaaa tccgctggga atttcagcgg attttgtttt ccataagcaa
     4921 aaaaaacctg ccagcaatgg caggtttttt ataactaaaa aacgaaatta atagtctttc
     4981 ggtgcgcggt tggcgcgacg acgatcgttt tccgtcaggt gacgtttacg aatacggata
     5041 gaggtcggag tcacttctac cagttcgtca tcatcgatga actccagagc ttgttccaga
     5101 gtcatgcgga taggcggaac cagaacaacg gcttcgtcag taccggaagc acgcatgttg
     5161 gtcagtttct taccggtcag gcagtttaca gtcaggtcgt tagagcggct atgaataccg
     5221 ataatctgac cttcgtaaac ttctgcaccg tgaccgagga acagcttacc gcgatcctgc
     5281 agaccgaaca gcgcgaacgc gaccgcttta ccctgaccgt tagagatcag tacgccgttc
     5341 tgacgctgac ccacttcacc cggacgtacg tcgtcgtagt ggctgaaggt ggagtacagc
     5401 agaccagtac cggaagtcat ggtcatgaac tcagaacgga agccaatcag accacggctt
     5461 gggatcacgt agtcgagacg tacgcggcct ttaccgtctg gattcatgtt tttcaggtcg
     5521 cctttacgtt cgcccagcgc ctgcattaca gaaccctgat gctgttcttc aacgtccaga
     5581 gtcacgtttt catatggctc ttgtttacga ccgtcgattt cacggaagat aactttcgga
     5641 cgggataccg ccagttcgaa accttcacga cgcatgtttt cgatcagaac agacaggtgt
     5701 agttcgccac gaccagaaac gcggaacgca tcagcgtctt cggtttcttc tacgcgcagc
     5761 gcaacgttgt gtaccagttc tttgttcaga cgatccagga tctgacgaga cgttacgaac
     5821 ttaccttctt taccgcagaa cggcgaggtg ttaacgcaga agaacataga aacggtcggc
     5881 tcatcaacgg agagtgccgg cagcgcttca acgttttgcg tgtcgcaaac ggtgtcagaa
     5941 atgttcagtt cgccaaggcc cgtgatcgca acgatatcgc cagcttccgc caggtcggtt
     6001 tcgatacgtt ccagaccgag gtggcccagc actttaccga ctttcgcgtt gcgggttttg
     6061 ccttcgctat cgatgatagt gacctgctgg ttcggcttca ctttaccgcg cttgatgcgg
     6121 ccaatgccga taacgccaac atagctgttg taatcgagct gagaaatctg catctggaac
     6181 ggaccgtcaa ggtcaacgtc cggcgcagga acgtggtcaa caatcgcctg gtacagcggg
     6241 gtcatgtctt ccgccatatc ttcgtggtcc agacccgcga taccgttcag cgcagaagag
     6301 taaacgatcg ggaagtccag ctgctcgtcg gtcgcgtcga ggttaacgaa cagatcgaat
     6361 acctgatcca caacccaatc aggacgcgcg ccagggcggt caactttgtt gataacaaca
     6421 atcggcttca ggccgtaagc aaacgctttt ttggttacga agcgcgtttg cggcatcggg
     6481 ccgtcaaatg cgtcaaccac cagcagcact gagtctacca tggacattac acgttcaact
     6541 tcaccaccga agtcggcgtg ccccggggta tcaactatgt tgatacggta atcattccat
     6601 ttgatagcgg tgtttttcgc gaggatggta atcccacgct ctttctccaa atcgttggag
     6661 tccatcacgc gctcttgggt ttcggcacga gagtcgaacg taccggattg ttggagcagc
     6721 ttgtctacca gggtggtttt accatggtct acgtgcgcga tgatggcgat attacgcaat
     6781 ttttcgatca caactttgcc tcaggcatta gaaatagcgc gttattgtac acggattaat
     6841 cgcactacaa aacaggatca caaacatcct ccgcaaacaa gtattgcaga gtccctttgt
     6901 gatcgctttc acggagcata aaaagggtta tccaaaggtc attgcaccaa catggtgctt
     6961 aatgtttcca ttgaagcact atattggtgc aacattcaca tcgtggtgca gcccttttgc
     7021 acgatggtgc gcatgataac gccttttagg ggcaatttaa aagttggcac agatttcgct
     7081 ttatcttttt tacggcgaca cggccaaaat aattgcagat ttcgttacca cgacgaccat
     7141 gaccaatcca ggagagttaa agtatgtccg ctgaacacgt actgacgatg ctgaacgagc
     7201 acgaagtgaa gtttgttgat ttgcgcttca ccgatactaa aggtaaagaa cagcacgtca
     7261 ctatccctgc tcatcaggtg aatgctgaat tcttcgaaga aggcaaaatg tttgacggct
     7321 cctcgattgg cggctggaaa ggcattaacg agtccgacat ggtgctgatg ccagacgcat
     7381 ccaccgcagt gattgacccg ttcttcgccg actccaccct gattatccgt tgcgacatcc
     7441 ttgaacctgg caccctgcaa ggctatgacc gtgacccgcg ctccattgcg aagcgcgccg
     7501 aagattacct gcgttccact ggcattgccg acaccgtact gttcgggcca gaacctgaat
     7561 tcttcctgtt cgatgacatc cgtttcggat catctatctc cggttcccac gttgctatcg
     7621 acgatatcga aggcgcatgg aactcctcca cccaatacga aggtggtaac aaaggtcacc
     7681 gtccggcagt gaaaggcggt tacttcccgg ttccaccggt agactcggct caggatattc
     7741 gttctgaaat gtgtctggtg atggaacaga tgggtctggt ggttgaagcc catcaccacg
     7801 aagtagcgac tgctggtcag aacgaagtgg ctacccgctt caataccatg accaaaaaag
     7861 ctgacgaaat tcagatctac aaatatgttg tgcacaacgt agcgcaccgc ttcggtaaaa
     7921 ccgcgacctt tatgccaaaa ccgatgttcg gtgataacgg ctccggtatg cactgccaca
     7981 tgtctctgtc taaaaacggc gttaacctgt tcgcaggcga caaatacgca ggtctgtctg
     8041 agcaggcgct gtactacatt ggcggcgtaa tcaaacacgc taaagcgatt aacgccctgg
     8101 caaacccgac caccaactct tataagcgtc tggtcccggg ctatgaagca ccggtaatgc
     8161 tggcttactc tgcgcgtaac cgttctgcgt ctatccgtat tccggtggtt tcttctccga
     8221 aagcacgtcg tatcgaagta cgtttcccgg atccggcagc taacccgtac ctgtgctttg
     8281 ctgccctgct gatggccggt cttgatggta tcaagaacaa gatccatccg ggtgaagcca
     8341 tggacaaaaa cctgtatgac ctgccgccag aagaagcgaa agagatccca caggttgcag
     8401 gctctctgga agaagcactg aacgaactgg atctggaccg cgagttcctg aaagccggtg
     8461 gcgtgttcac tgacgaagca attgatgcgt acatcgctct gcgtcgcgaa gaagatgacc
     8521 gcgtgcgtat gactccgcat ccggtagagt ttgagctgta ctacagcgtc taatagttga
     8581 agttgtacta cccggcgcaa caacgccggg atttagttgc cgtggaaact ttcagcccat
     8641 ctctgcatgg gcttttttct ccgtcaattc tctgatgctt cgcgcttttt atccgtaaaa
     8701 agctataatg cactaaaatg gtgcaacctg ttcaggagac tgctttatgg caacaggcac
     8761 gcagcccgat gctgggcaga tcctcaactc gctgattaac agtattttgt taatcgatga
     8821 caacctggag atccattacg ccaaccctgc cgcgcaacaa ctgctcgccc aaagctcccg
     8881 caaattgttt ggtacaccgt taccggaact gttgagctac ttctcattaa atatcgagct
     8941 gatgcaagaa agtctggagg cggggcaagg ttttaccgat aacgaagtga cgctggtcat
     9001 cgacgggcgc tcgcatatcc tttctgtgac tgcccagcgt atgccggacg gcatgatcct
     9061 gctggagatg gctccgatgg ataaccagcg ccgcttaagt caggaacagc tacagcacgc
     9121 ccagcaggtt gctgcccgtg atttagtgcg cggcctggca catgagatta aaaatccgct
     9181 tggcggttta cgtggcgcgg cgcagctgct cagcaaagcg ttacctgacc catcactact
     9241 cgaatatacc aaagtgatta tcgaacaggc ggaccggctg cgaaatctgg tcgaccgtct
     9301 gttggggccg cagctgcccg gtacgcgcgt taccgaaagt attcacaaag tggctgaacg
     9361 cgtggtaacg ctggtgtcga tggaactgcc ggacaacgtg cggttgattc gtgattacga
     9421 tcccagcctg ccggaactgg cgcacgaccc ggatcaaatt gaacaggtct tgctgaatat
     9481 tgtgcgcaat gcgctacagg cgctggggcc ggaaggcggt gaaatcattc tgcgtacccg
     9541 caccgcgttt caactgacct tacacggcga gcgctaccgg ctggcggcgc ggattgatgt
     9601 ggaagataac gggccgggca ttccgcctca tttgcaggat acgctgtttt acccgatggt
     9661 cagcggccgc gaaagtggca ccgggcttgg cttatccatc gcccgtaatt tgattgatca
     9721 gcattcaggc aaaattgaat ttaccagttg gccaggtcat accgagttct cggtttacct
     9781 gcctatcagg aaataaaggt gacgtttatg caacgaggga tagtctgggt agtcgatgac
     9841 gatagttcca tccgttgggt gcttgaacgt gcgctcgctg gagcgggttt aacctgtacg
     9901 acatttgaga acggcgcgga agtactggag gcgctggcga gcaaaacgcc ggatgtgctg
     9961 ctttcagata tccgtatgcc gggaatggac gggctggcgc tgctcaagca gattaaacag
    10021 cgccatccaa tgcttccggt catcattatg accgcacatt ccgatctgga tgctgccgtc
    10081 agcgcctatc aacaaggggc gtttgattat ctgcccaaac cgtttgatat cgacgaagcc
    10141 gtggcgctgg ttgagcgcgc tatcagtcat taccaggaac agcagcagcc gcgtaatatt
    10201 cagcttaacg gcccaacgac cgatatcatc ggcgaagcgc cagccatgca ggacgtgttc
    10261 cgtattatcg gtcggctttc gcgttcttct attagcgtgc tgattaacgg cgaatccggc
    10321 accggtaaag aactggtcgc tcatgccctg catcgccaca gtccgcgcgc caaagcgccg
    10381 tttatcgcgc tgaatatggc agctatccca aaagatttga tcgaatcaga actgtttggc
    10441 cacgagaaag gcgcgtttac tggcgcgaat accattcgtc aggggcgttt tgaacaggcc
    10501 gatggcggta cattattcct cgacgaaatt ggtgatatgc cgctggatgt gcagacgcgt
    10561 ttgctgcgcg tgctggcaga cggtcagttt taccgcgttg gcggctatgc gccggtgaaa
    10621 gtggatgtgc ggattatcgc tgccactcac cagaatctcg aacagcgagt gcaggaaggt
    10681 aagttccgtg aggatctgtt ccaccgccta aacgttatcc gcgttcatct gccgccgctg
    10741 cgcgaacgtc gggaagatat tccccgtctg gcgcgccatt ttttacaggt tgccgcgcgc
    10801 gaactgggcg tagaagcgaa gttactgcat ccggaaaccg aagctgctct gacgcgtctg
    10861 gcgtggccag gcaacgtgcg ccagctggaa aacacctgcc gctggctaac ggtgatggcc
    10921 gccgggcagg aagtgttgat tcaggatttg cccggcgaac tgtttgaatc aacagttgcg
    10981 gagagtactt cgcaaatgca accggacagc tgggcgacgc ttcttgcgca gtgggcagac
    11041 agagcgctgc gttccggtca tcaaaatctg ctttccgaag cgcagccaga gctagagcgg
    11101 acgttactga cgaccgcgtt gcgacatacg caggggcata aacaggaagc ggcgcggcta
    11161 ctcggctggg gccgcaacac cctgacgcgt aagttaaaag agctggggat ggagtgattc
    11221 acagcttgtg tgtaaagatt gattattgag cgcaaattgc tggtatttta cgctttactg
    11281 ttccgataag ttcagtatga tctgcccgga aaacggggag agtcattatg ctggaatcaa
    11341 taattaatct ggtatcgagt ggcgcagttg acagccacac accgcaaact gccgttgctg
    11401 ccgtgctgtg tgccgcaatg attgggctgt ttagttgagt ttcagattgc tgatgaagtg
    11461 ctctttgttc atgccggatg cggcgtgaac gccttatccg gcctacaaaa gcaatatttt
    11521 caacaggttt tgtaggcctg ataagcgtag cgcatcaggc aatcttgttc tcagcacatt
    11581 gatggcgctt tcgttttcta ctttgtaaac gaagcgccat tcactattca aatcacccgt
    11641 gagaactgct gcatccgcgc tttctggcgc agataggtat caaagcacat gcaaatgttg
    11701 cggatcagca agcgaccttt cgccgtcacc tgtattccct tctcatccac atccaccagc
    11761 ccatcttttg ctaacggggc gagcagcttg agatcttccg caaagtaatc agcgaagtgc
    11821 aaatcccact gtttctcaat aggggcgtaa tccagacgga agttgcagat gagcgactta
    11881 atcacatcgc ggcgaataca gtcatcacgc gttagcgcaa taccacgcca cagcgcattg
    11941 ccttgttcat ccacttgctg atagtactgc ttcaactctt tctggttctg cgcgtagcag
    12001 tcgccaatca tgctgatggc ggaaacgccc atccccagca gatcggtatc gccctgagtg
    12061 gtgtagccct ggaagttacg atgcagcacg ccttcacgct gggccaccgc cagctcgtca
    12121 tccggacggg caaagtgatc cataccgata aactgatagc ccgattgcgt caggaaggcg
    12181 atggtttcct gcaggatatc gagtttttgc tgcggactcg gcaggtcagc atctttgatt
    12241 ttgcgctgag cagcaaaaat ggtcggcaga tgcgcgtagt taaagacact cagacgatcg
    12301 gggttcagct ccgccacacg tttcagggta aaggcgaaac tctccggcgt ctgtttcggc
    12361 aggccgtaaa tcaggtcgat gttggtggag gtaaaaccaa tctcacgcgc atggttaagc
    12421 agtgcaaaga tgaactcttc atcctgctcg cggttaacca gacgttgcac ttctttgttg
    12481 aagtcctgca cgcccatgct caggcgatta aagccttcgg cgcgtaaatg atcgagtaca
    12541 tccagttcga tttcccgcgg atcgacttcg atcgaaatct ccgcatcggc attgaactgg
    12601 aagttttcgc gcagcagctt catcaggcgg ctgatttgcg ctttattcag atacgtcggc
    12661 gttccgccgc cccagtgcaa ttggctgacg tgacgcccgg cgaacagcgg tgcacgatgg
    12721 acgatttctt gctccagcgc gtccagatac tgatcggcct tgtgctgctg gcgagtaaca
    12781 atcttattgc aaccgcagaa gtaacaaagc ttatggcaga acgggatatg tacgtagaga
    12841 gataatggac gctcaggata gcactccacg gcttgtaaaa acgcctgttc gccgaagtct
    12901 tctgaaaact ccagcgcggt cgggtacgag gtgtatcgtg gcccggaata gttatatttc
    12961 tggatcaggg ccagatccca gtcgatttgc tgtacagaca tgctcactcc ttccgatggc
    13021 gtctctggcg gctacggcgt accggcggat gcccgtttcg ggtcatcagc cggttgcgca
    13081 gccacttttg tcgccgcgac aaccgctgta gtttaacgaa taaccacacc agataacata
    13141 taaccggaag ggttatcagc agaacgggaa ggcccacggc ggcaatccgt tagttgcccc
    13201 gcagcagacg catcatgtct tcttgcttct cgtcttcttc ctcttcttcg tcatcgtcat
    13261 aagagaggcc gagtttctgc atcaactcat caatacgatc cagtttagca tccacccagg
    13321 attgctcttc ggcactcagg gtttcgcctg cttccagacg ttccagcagc gcatccagac
    13381 gctcatccgt ttccagtaat tccaactccg cctgcggtga aagcataggt ttctcactct
    13441 tcggtttgtg ctgtttggtg actttttcag tcacgcccaa tggaataggg gttttactgc
    13501 caatacgtgg atcttttggt gcgttctggc ctttgctgcc tgacgtggtg tttccgcccg
    13561 ctgcgcggct gcccggcgca tgaccacgac gttttttctg acgcttgcga tcacgagctt
    13621 cctgatccag ctcctcgcgt gtttttcgac gcgcttttgc atgacctttg ctgcgtgagt
    13681 ttgaagatga tggtttcata atggttcgtc ttttagccgt tttattcagt atcgatttgc
    13741 ggcggaatct aacagaaagc aagcaaagaa aaaaggcgac agattactct gtcgcctttt
    13801 ttcctgactc ataaccctta acgggtctta caatccttgc aggccgccaa cacaccctgt
    13861 ttttccttta gccggaacaa tccgtgttga ttccatttcc gtttaattac gtctccggac
    13921 gtttgtcttc ctgacaatcc tctgtcttcg cctcctggcg ctcctgaccc tcatcctgag
    13981 tcattgttcc tgttagcgtc ctcgctctat gggtgctact ttacgccttt gctttaaaca
    14041 aacaatcaac cgccatcagt atttgcacat tttcagagtt ttatataaaa gcaattcact
    14101 gatttatata gcttatttga catgcatcgc agaattttct ctgctatttc gcataaagac
    14161 tatcggcaat gtctcacaaa gcgtatggct tttacttaca acgctcacgc cgaaaaatcc
    14221 gccttttatt atggttcagg tataatcgcc acaaagcttt gactgacgga gacgaccgct
    14281 ttgactaatt tgaattatca acagacgcat tttgtgatga gtgcgcctga tattcgccac
    14341 ctaccttccg ataccggaat tgaagtggct tttgcaggcc gttccaacgc aggtaaatcc
    14401 agcgcgctga acacgctgac taaccagaaa agcctggctc gtacctcaaa aaccccaggg
    14461 cgcacccagc ttatcaacct gtttgaagtg gctgacggca agcgtctggt tgacttgcct
    14521 gggtacggtt atgcggaagt cccggaagag atgaagcgca aatggcagcg tgcgctcggc
    14581 gaatacctcg aaaaacgtca gagcctgcaa ggtctggtgg tgctaatgga tattcgccat
    14641 ccgctgaaag atttggatca gcagatgatt gagtgggcgg tagacagcaa tatcgccgtt
    14701 ctggtgctgc tgaccaaagc ggacaaactg gcaagcggcg cacgtaaagc gcaattgaat
    14761 atggtgcgtg aagctgtact ggcgtttaac ggtgatgtgc aggttgaaac gttttcttcg
    14821 ttgaagaaac aaggcgtgga caagctgcgg cagaaactgg atacctggtt tagcgagatg
    14881 cagcctgtag aagaaacgca ggacggcgaa taattttctt gccttaatgc ttgtgccgga
    14941 tgtggcgtat ccggcccgta aattcaatag attacaattc taccgttggc ctgataaaca
    15001 tagcgcatca ggcattacgg atcttttctt tcgcccaata aaaaacgccc cagtcattac
    15061 tgactggggc ggctaaaata ttcagccaaa tccgattacg tgaagtaaaa ggtctgaaag
    15121 atagaacatc ttacctctgt accctacgcg actaacttta ctcttttttg ttcagttcag
    15181 aaagcacttt ttgtaatttt ttttcagttg ttgcatagga atttctaata gccatcacaa
    15241 aacgtgacag cttatgttgc ttacttacga aaaaagggca tgttcaggcg aatcttagtg
    15301 cgcctgatcc cagttttcgc cactccccac ttccaccagc aacggcacat ccagacgggt
    15361 acagttttcc atcagttgat gaatctgctt cgcgacggca tcaacatcat ctttatgaac
    15421 ttcaaatacc agttcatcgt gtacctgcat gatcatacgt acacgcggtt gctcagcctg
    15481 taaccacgca tcaacggcaa tcatcgcccg tttgataatg tcggcggcgg ttccctgcat
    15541 tggcgcgtta atggctgcac gttcagccgc tgcacgacga gcaccattgc tggatttgat
    15601 atccggcaga tacagacggc gtccgtccag cgtttcaacg tagccctgct ctttcgcctg
    15661 agcacgggtg cgttccatat actccagcac gccagggtag cgttcgaagt aaaggtccat
    15721 gtacttctgc gcttctttac gtggaatgtt caattgccgc gccagaccga aagcactcat
    15781 gccataaatc agaccaaagt tgatcgcttt cgcgctacgg cgttgctcgc tggtgacggt
    15841 ttccagtggc aaaccaaaca cttctgccgc cgttgcccgg tggatatctt ttccttccgc
    15901 gaatgcggtc agcaagcctt tgtcacgcga aagatgcgcc ataatgcgca gttcaatctg
    15961 cgagtagtcc gctgagacaa tcacataatc ctctggcgca ataaacgcct ggcggatacg
    16021 acgaccttct tcgttacgca ccggaatgtt ttgcaggtta ggatcggttg acgataaacg
    16081 tcccgttgca gttactgcct ggtgataaga ggtatgcaca cgcccggttt tcgggttgat
    16141 catcagcggc agcttgtcgg tgtaggtcga tttcagcttc gccagaccac gatactccag
    16201 aatcactttt ggcaacggat agtccagcgc cagttcttcc agtacctctt ccgacgttga
    16261 cggcgcgcca cccggcgttt tcttcagcgg tttaataccc tgtttttcaa acagaatggt
    16321 ttgcaactgc ttggtggaag aaaggttaaa ttcttcacct gcaatttcat gcgctttctt
    16381 ttccagctca gccagacgca aggtgagctc ttcagaatga ttgtgcagca ctttcggatc
    16441 gatcttcaca ccgttacgtt caatgcgtga aagcaccggc accagcggca tttcgatatt
    16501 ctcgaagacg ttcaacggcc ctttgtgttt ttgcaggtcc ggccacattt tcagatgcaa
    16561 ctgcaaggtg acatcagcat cttcggcggc gtaacgtccg gcctcttcga gggcaatctg
    16621 gttaaaggtc agttgatttt tgcctttacc cgcaatctct tcaaaagtga tggttttgtg
    16681 cttcaaccaa cgttccgcga ggctgtccat atcgtgacgc ccggcaacgc tattgagaat
    16741 gtaggactcc agcatggtat caaacgcaat cccacgcagc tcaatgccgt aattcgccag
    16801 aataccgcga tcgtatttca ggttttgccc gaccttcagc gccttttcat cttccagcag
    16861 cggttttagc aactcgagtg cacgctcgcg agagatttga tcgggcgcat caagataatc
    16921 atgagcaacc ggaatatatg ccgctacgcc tggctcgata gcaaaagaaa gcccgaccag
    16981 gttagcagag atgttatcaa ggctgtcggt ttcggtatca aatgcaaata ccggcgcttt
    17041 ttccagcttc gcaatccacg ctttcagtgt ttcttcatca aggatggtga cgtagttgtc
    17101 ataagaaatc accgttgccg tcacttctgg cgcttcgtct gcaacactgg tttcctgcgg
    17161 cttcgcggct gtttttgccc ctttggcctg taaccatttg cccgcttcga catcagcagt
    17221 ccagcgtttg aactcatact ttttgaacag ccccaacaac tcttccgctg ccggttgctg
    17281 cacttccagt tgttcacagg tcagctccag ttcaacgtcg gttttaatcg tcgccagctg
    17341 gtatgagaga taagcaactt ctttgttttg ctcgagcttc gctgccattg ttttcgcgcc
    17401 acggaagctc aacccagcaa ttttttctgg ctcggcatac agcgtatcca gcccgcccag
    17461 accttgcagc aatgcctgcg cggttttttc accgacgccc ggtacgccag ggatgttatc
    17521 ggaggagtca cccatcagcg ccaggaaatc gatgatcagt tctggcggca cgccgtactt
    17581 attcaccacc tcttccggtc cgaggatggt attcgtcatg gtattgataa gcgtaatatt
    17641 tggcgtcacc agctgcgcca tatctttatc gccagtgctg atcagcaccg gacgcccggc
    17701 tttttcggct tcgcgcgcca gagtaccgat aacgtcgtcc gcttctacgc cagaaaccgc
    17761 cagcagcggc agtcccatcg ctttaaccat cgcgtgcaga ggttcgattt gtgctcgcag
    17821 atcgtccggc attggcgggc gatgtgattt gtaatgttca aacagttcat cacgaaaggt
    17881 ttttcccttg gcgtcaaaga ccaccgctgc atgcgtcggt ttatattgca tgatcagact
    17941 gcgcagcatg ttgaggacac catacatcgc accggtcggc tcgcctgcgc tgttagtcag
    18001 cgggggaaac gcgtgatatg cgcgataaag ataagatgaa ccatctacaa ggataagtgg
    18061 attttggggg atctgaacca taatgtccgt gcctgtttat cagattatgg gtaaaggatg
    18121 ccacagacag gataaaaaca tgagtttttc accgcttttc gtgaaaatct tttgtagatc
    18181 ttctggatcg ctcgcaaatc gtcatgtgga taactttgtg cataaattat ttacatcgct
    18241 tttaatttat tatcatgctt tttacagtat gatgtttgtc tttgtttttc atgtagttaa
    18301 aaataaagcc atccctaata attgagataa tgggattttt tctaatgtgg atagatatga
    18361 attatttttc tccttaagga tcatccgtta tttgggtcgt tttctgctaa aatctgcgcc
    18421 ttgcgcttct ttagcgcact gtctatggct aaccttttga ataaattcat tatgacgaga
    18481 atactcgctg cgataaccct tttgctgagt atcgtattga ccattttggt cactattttt
    18541 tgttccgtcc cgatcatcat tgccgggatt gtaaaactat tgctacctgt gccagtcatt
    18601 tggcgaaagg tttcgcgttt ttgtgatttt atgatgtatt gctggtgtga aggtctggcg
    18661 gtattactgc atctgaaccc acacctgcaa tgggaagttc acgggctgga agggttaagt
    18721 aagaagaact ggtatctgct tatttgtaac caccgtagtt gggcagatat tgtcgtactg
    18781 tgcgtgctgt ttcgtaagca cattccgatg aataaatatt tcctcaagca acagctggcc
    18841 tgggtgccat ttcttggcct ggcgtgctgg gcgctggata tgccgtttat gaagcgttat
    18901 tcccgcgctt atttattacg tcatccggag cgacgtggta aagatgttga aaccactcgg
    18961 cgttcttgtg aaaagtttcg cctgcatccc accaccattg tcaatttcgt tgaaggctcc
    19021 cgtttcacgc aagaaaaaca tcagcaaacc cactccactt ttcaaaactt gttgccacca
    19081 aaggcggcag gcattgcgat ggcgataaat gtactgggta aacaattcga taaactgctt
    19141 aatgttacgc tgtgttatcc ggacaataac cgtcagccgt tcttcgatat gttaagcggt
    19201 aaactgacgc gaattgtcgt tcatgtggat ttacagccta ttgccgatga gttacatggc
    19261 gactacatca atgacaaaag ctttaagcgc cattttcagc aatggctgaa ctcactgtgg
    19321 caggaaaaag accgacttct gacgtcgttg atgtcatcac agcggcagga caagtaaatt
    19381 ttgctcacct ctgaatgagc agaggcgagt gagtatttta atgaacaaaa cgtccggcac
    19441 gagacataaa ttcttcttcg ctcatctcct gtccgttaaa aacaacttta cccggtgtat
    19501 aacgcagttg cagcgaggcg gtattgttgt caatcgtgat taactgaaac atctgaccta
    19561 aggtcatcat cccgctgatt tgtttatcag cccgcttttg cgctttttcc gcatccattc
    19621 cttcagataa attaagctgt tttgcggttt ctgtgacgac attaagcggt aacttcacat
    19681 caaaattgag cgatttgata tctttgtttg tggatgatga agatttggct gggtcggcaa
    19741 tactgatatc cagattggca tttagttcgc cgagtgcgtt cttccatctt accggttgtt
    19801 taatggtcgg ctcactttgt tgtaataacg gtaaatactc tttgaacaat gcagcattca
    19861 cttcttgcag agcaacttca tcgtttgcta actcagggtg tgcagcaagc tgcttctgca
    19921 tcgcaatgtt atactggatg ataaattggc gcactgctga gggataaata gattcagcaa
    19981 tcaatgacca ctcaccacta ccgagttgct gattcccttt tgttaactta tcaagttcgt
    20041 aggtcagatt gacattgata taatctttgt ctttaacgcg atccagcctg gtttttgcaa
    20101 cgaaagcatc gatttgggca acgtcttccc cctgattgat aatattaacc tgattaaatt
    20161 tcgactcgct ttcaccaacc ggaaatttct tttcttctaa tcgtgtcaga gaatcaatgg
    20221 taaaactttt tgccgttact ttgttcattg gtgataattg cagaacaata tcttcgactt
    20281 tgccttcaat atccaggtta gacaatgaac taccatcacc attaaaagta aactggccct
    20341 caccaaaaga aaactttcca tactctgcag gggcaacatc taatgtcgta gagaatgagc
    20401 caccaaacgc gaatcgcaat gtcgcttcca cgggcgattt atttttcgcg gcgataaata
    20461 gcggttgtgt cagttcatta ttcactaagt tcacttttgc cgctgccagt gctgggatga
    20521 cattaccatg cattaacatt gtgatgggca acggaccgtg ctcaacatcc acatcaaatg
    20581 caactttctg gcctggcttg atattgagat cgggtgcacc gttatcaaat gaaatttgca
    20641 tttgaaaatg ggaattaaaa aagcctttgt caaagttttt ataacttaac gtcatatcgt
    20701 aggcatgttc acctttcttt ttagcatcgt taaaatcttt aataaatttt tcgacaccag
    20761 gctgaatttg cgtacctgtg taccatgtgc caccacccca gattacggct aacgcaacaa
    20821 taacacctgt agctgactta cgtatcatat aaatattccc tgataaaaca gcaaaacttt
    20881 gaatatccac ttatgctgaa ataatgtcca gcgacaggat gtattatcag aatgtattta
    20941 tatttattaa ataataaaaa aagcccgtga atattcacgg gctttatgta atttacattg
    21001 aattattttt tctcggacag atatttcact gtatcagcat actgctgaac aaaaacatcc
    21061 atattgctgg tatccatacc ctgcggattc agctgatatt taccgttaac aaacatcgcc
    21121 ggaacaccgc gcaattgcac gtcagctgca gctttttcct gctgagctac cagagatttc
    21181 accacgaagc tgttccacgc cgcgtcgtac tcttcacctt taatacctgc gttgataaat
    21241 acatcgcgga tatcagaagc agaacgaatg gtctgggttt tctgtacgcc ttcaaacagc
    21301 ggaacagtca ctttgtcttc cacgcccagc gccatcgcca cagcccatgc ctgagtcaga
    21361 tctttgccca ggtcaccacc catgaagttg acgtggtatt tagtcatctt cacgccttcc
    21421 ggcagttttt tcttcacatt atcagaaata tgcagaactt cttcaaactg atagcagtgc
    21481 ggacagaaga aagagaaaaa ctccagcact tgcggcgcgc cagctaccgg tttttccagg
    21541 gtagtgtact gtttaccatc ttcatactgc gccgccgatg cgctaaacgc taaaactaaa
    21601 ccagccagcg ccagccaaat ctttttcatg atctactctc tccgattaat acataggtgt
    21661 taattgtaaa gggggttctt gtagaacttt tgcctgttct ataaaagtcg ccgtttgtcg
    21721 cagccagtaa tcttccccgg ttaaccacgg gaaatttttc gggaacgcgg gatcagccca
    21781 acgccgcatt agccaggcaa gataataaac caaacgcatg gcgcgtaaag gttcaatcag
    21841 tccgatttca gcggtgtcga actcgctaaa ttcttcataa gcttcaataa tagtttccag
    21901 ttgcatccgc tgctcggctt tatcgccatt gagcaacatc cacaaatcct gaatagctgg
    21961 accattacgt gcatcatcca gatcaacaaa cattggacca tcgcgccaga gaatattccc
    22021 ggcgtggcag tctccatgta agcgcagaac ggtggaatct tcccgccagt gcgctgtaac
    22081 ggcggcgatc agctcatctg tcgctttcag gaatgccgct ttcaacccgg aaggtatcag
    22141 tgtagcgtcc tcaaacagct tgcgtggctc aatgagatat tcattcagac cgatggtcgg
    22201 gcgatggata aaaagctgtt tgcgccccgt ctggtgcata cgccctaaat aacgcccaac
    22261 cgcttccatc tgatcgatat tatcagcttc gaactggcga ccaccgacgc ttggaaaaac
    22321 agcgaaataa aatccctgat gattcaataa agtctgaccg ttaaaggcca caggtgctgc
    22381 gaccggaact tcatcattta ccaactgcaa cgcaaattga tgttcttcga ggatttgatc
    22441 ggctgtccaa cgttcagggc gataaaattt gacgacaata cgtcgaagat cttcgtcctg
    22501 aaattgatag acacggtttt catagctgtt aagcggggta agaccggaat ccacccggat
    22561 cccttgctca aatagagcat ccatgatggt atccgggtgt agtgtctgga aagtaaaagc
    22621 gctgttgttc atcccatcat ccggaaaaca ttacgaataa tgaaggatat catttcacga
    22681 tgattttggt ggcgccgctt acaagctttt actctttaat tacgccgcga gcgcgtaaca
    22741 gcgccgtttt gaagtcttct tcataatctt tctgcaaacc cgggatcacc gcatctttcg
    22801 cggaatcacg cattttcagg tgatagatca gaatgtcatc cgtcaaatcc gccagttcgc
    22861 cgtcaaaacc tgactctttc gccagtttct gcaaaaattg caacaggtta aggtctggct
    22921 ctttttgcca ggctggctgg aggaattcaa taacttcatt cagacgttta catttcatag
    22981 tgatgctcct tactcttgag acagacacgt tagcagggtc aatcccacaa taaaagaggc
    23041 gatatcggtg aatctgatga cgacgataac aggcgttgtg ctggcaggcg gtaaagccag
    23101 acgaatgggc ggcgtagata aaggattgct tgaattaaac ggcaaaccat tatggcaaca
    23161 tgtcgctgac gcgcttatga cgcagctctc tcacgtcgtg gttaatgcca atcgtcatca
    23221 ggaaatctat caggcaagcg gtctgaaagt gattgaagat tcactggcgg attacccagg
    23281 ccctctggca ggaatgcttt cagtaatgca gcaggaagct ggtgagtggt ttttgttttg
    23341 cccgtgcgat acgccttaca ttccccctga tttagcagcc cggcttaatc atcagcgcaa
    23401 agatgcgcct gtcgtgtggg tccatgacgg tgaacgcgat cacccgacta ttgctctggt
    23461 aaaccgcgct attgagcctt tattactgga atatctgcaa gcaggagaac gccgggtaat
    23521 ggcatttatg cgtctggctg gcggtcatgc ggttgatttc agcgatcata aagatgcatt
    23581 tgttaacgtg aatacgccag aggagcttgc ccgatggcag gaaaaacgat gataccgtta
    23641 ctcgcctttg ccgcgtggag tggtaccgga aaaactacgc ttttgaaaaa actgatcccg
    23701 gcattatgcg ccagagggat ccgtccaggg ctgattaagc atacgcacca tgatatggat
    23761 gttgataagc caggcaaaga tagctatgag ctgcgcaagg ctggcgcggc acaaaccatc
    23821 gttgccagcc agcaacgatg ggccttgatg acggaaacac cagacgaaga agagctggat
    23881 ctacagtttc tcgcaagtcg aatggatacc tcaaagctgg atttgattct ggtcgaaggg
    23941 tttaagcatg aagagatcgc aaagattgtg ctgtttcgcg atggagccgg acatcgacct
    24001 gaagaattag tgatagacag gcatgttatt gctgtagcca gtgatgtgcc gcttaatctt
    24061 gatgtcgcat tactggatat taatgatgtt gaggggctgg ctgattttgt ggtggagtgg
    24121 atgcaaaagc agaacggata agcaagagta ggctggatca gacgcagcca g
//
LOCUS       DAJTNA010000047        24033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTNA010000047 DAJTNA010000000
VERSION     DAJTNA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24033
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.048"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..302)
                     /locus_tag="OO161_001581"
     CDS             complement(<1..302)
                     /locus_tag="OO161_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR5462702.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANT"
     gene            complement(586..876)
                     /gene="ubiK"
                     /locus_tag="OO161_001582"
     CDS             complement(586..876)
                     /gene="ubiK"
                     /locus_tag="OO161_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5462703.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     regulatory      998..1146
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1250..1903
                     /gene="ribB"
                     /locus_tag="OO161_001583"
     CDS             1250..1903
                     /gene="ribB"
                     /locus_tag="OO161_001583"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5462704.1"
                     /translation="MNQTLLSSFGTPFERVENALAALCEGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            2164..2334
                     /locus_tag="OO161_001584"
     CDS             2164..2334
                     /locus_tag="OO161_001584"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462705.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(2392..3153)
                     /gene="zupT"
                     /locus_tag="OO161_001585"
     CDS             complement(2392..3153)
                     /gene="zupT"
                     /locus_tag="OO161_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5462706.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSNGVLCGMSVMGFSLVLLQTAGIG"
     gene            3296..4084
                     /gene="ygiD"
                     /locus_tag="OO161_001586"
     CDS             3296..4084
                     /gene="ygiD"
                     /locus_tag="OO161_001586"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5462707.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(4122..5282)
                     /locus_tag="OO161_001587"
     CDS             complement(4122..5282)
                     /locus_tag="OO161_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5462708.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(5288..5959)
                     /locus_tag="OO161_001588"
     CDS             complement(5288..5959)
                     /locus_tag="OO161_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5462709.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(6107..7588)
                     /gene="tolC"
                     /locus_tag="OO161_001589"
     CDS             complement(6107..7588)
                     /gene="tolC"
                     /locus_tag="OO161_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5462710.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7793..8422
                     /gene="nudF"
                     /locus_tag="OO161_001590"
     CDS             7793..8422
                     /gene="nudF"
                     /locus_tag="OO161_001590"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5462711.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            8423..8845
                     /locus_tag="OO161_001591"
     CDS             8423..8845
                     /locus_tag="OO161_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5462712.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            8870..9697
                     /gene="cpdA"
                     /locus_tag="OO161_001592"
     CDS             8870..9697
                     /gene="cpdA"
                     /locus_tag="OO161_001592"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5462713.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9697..10278
                     /gene="yqiA"
                     /locus_tag="OO161_001593"
     CDS             9697..10278
                     /gene="yqiA"
                     /locus_tag="OO161_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5462714.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            10307..12199
                     /gene="parE"
                     /locus_tag="OO161_001594"
     CDS             10307..12199
                     /gene="parE"
                     /locus_tag="OO161_001594"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5462715.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLMQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(12247..12561)
                     /locus_tag="OO161_001595"
     CDS             complement(12247..12561)
                     /locus_tag="OO161_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5462716.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12592..13173)
                     /gene="mdaB"
                     /locus_tag="OO161_001596"
     CDS             complement(12592..13173)
                     /gene="mdaB"
                     /locus_tag="OO161_001596"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5462717.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            complement(13283..14632)
                     /gene="qseC"
                     /locus_tag="OO161_001597"
     CDS             complement(13283..14632)
                     /gene="qseC"
                     /locus_tag="OO161_001597"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5462718.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNSPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(14629..15288)
                     /gene="qseB"
                     /locus_tag="OO161_001598"
     CDS             complement(14629..15288)
                     /gene="qseB"
                     /locus_tag="OO161_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5462719.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15440..15832
                     /gene="ygiW"
                     /locus_tag="OO161_001599"
     CDS             15440..15832
                     /gene="ygiW"
                     /locus_tag="OO161_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5462720.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQRPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15885..16367
                     /locus_tag="OO161_001600"
     CDS             15885..16367
                     /locus_tag="OO161_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5462721.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            16913..19171
                     /gene="parC"
                     /locus_tag="OO161_001601"
     CDS             16913..19171
                     /gene="parC"
                     /locus_tag="OO161_001601"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5462722.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19404..20141
                     /gene="plsC"
                     /locus_tag="OO161_001602"
                     /gene_synonym="parF"
     CDS             19404..20141
                     /gene="plsC"
                     /locus_tag="OO161_001602"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5462723.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20216..21628
                     /gene="ftsP"
                     /locus_tag="OO161_001603"
     CDS             20216..21628
                     /gene="ftsP"
                     /locus_tag="OO161_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5462724.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLIENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21739..23952
                     /locus_tag="OO161_001604"
                     /pseudo
     CDS             21739..23952
                     /locus_tag="OO161_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
BASE COUNT         5672 a         6013 c         6420 g         5928 t
ORIGIN      
        1 gtattcgctg tggtgaaagt aaccttcgct tttgaataag tggttccacc ttggctcaac
       61 atgcagtctt gcaggtgaat agtgaaaggc tcagccaaag atttctggtc gcttttcaga
      121 acggtatctg ctacttcacc cagatttaca gttaagtctt cgtcacctgg tttaattgaa
      181 catggagcag agataacttc acctttaaat ttaacaatgc cgtgaccttc gtcagcaaat
      241 acgttattgg aaagaatcat tgctacgcca gcaacaagaa gtgacttttt aaaagcagac
      301 ataatattcc tttatataaa tataagtaga aaatgaatga ttagtaactg ttatttaagg
      361 attgctaatc attcaaatat tatattttac ttttcgggtt ttatgaaatt agtttatcta
      421 aatatggatt aagtaaaact taaaaaaata cataacaatt atattttcaa ttgcactcat
      481 tcatttttgt gatgatattc tgaatggaat taagcgataa ggtaatagaa agcactgtaa
      541 attagaagat taattcataa tttacagtgc attaaagaca aatgtttaca gcgttggggg
      601 gagagtctct ggatctggct gctttttaat ctcagtactg cgattttcca gttcgctcat
      661 tctttgttca agcagcgcca gtttctcacg agtccgtaac aggacttgcg tttggacgtc
      721 gaactcttca cggcttacaa ggtcgaggcg cgtcagctga gcttgtaggg tttggcggat
      781 ttttttctcc acatcttccc cgaactccct gattccttta ggcattgatt cgtgaacctg
      841 gcgagcgatt tgctcaattt ttttcgggtc aatcattgta gtttccctgt actgataggt
      901 gttgagtacc attgtagtgc gataagggta agtcataaac cagaattatg tgaagctatg
      961 cgttgctgcc gctaatcatt agcgttatag tgaatccgct tattctcagg gcggggcgaa
     1021 attccccacc ggcggtaaat caactcagtt gaaagcccgc gagcgctttg ggtgcgaact
     1081 caaaggacag cagatccggt gtaattccgg ggccgacggt tagagtccgg atgggagaga
     1141 gtaacgattc tgtcgggcat ggacccgctc acgttatttt ggctatatgc cgccactcct
     1201 aagactgccc tgattctggt aaacataatt ttagtgaggt ttttttacca tgaatcagac
     1261 gctactttcc tcttttggta cgcctttcga acgtgttgaa aatgcactgg ctgcgctgtg
     1321 tgaaggacgc ggtgtaatgg tgcttgatga tgaagaccgt gaaaacgaag gcgatatgat
     1381 cttcccggca gaaaccatga ccgttgagca gatggcgctg accattcgcc acggtagcgg
     1441 tattgtttgc ctgtgcatca ctgaagatcg ccgtaaacaa cttgatctgc caatgatggt
     1501 agaaaataac accagcgcct atggcaccgg ttttaccgtg accattgaag cggctgaagg
     1561 tgtgactacc ggtgtttctg ccgctgatcg tattacgacc gttcgcgcag cgattgccga
     1621 tggcgcaaaa ccttctgatc tgaatcgccc gggccacgtt ttcccacttc gcgcgcaggc
     1681 aggtggtgta ctgacgcgtg gcggtcatac tgaagcgact attgatttga tgacgctggc
     1741 aggctttaaa ccggctggtg tgctgtgtga gctgactaat gacgatggca cgatggcgcg
     1801 tgcaccagag tgcattgagt ttgccaataa acacaatatg gcgctcgtga ctattgaaga
     1861 cctggtggca taccgtcagg cacatgagcg taaagccagc tgaaaaccgc tgcttaatta
     1921 ctgccttaat caagaaaccg aagttgtagc tggcttcggt ttttattttg ccctgctatg
     1981 caagatgtta attccgcttc tctttctatg agaaaatttc attaatatca ggcattcttt
     2041 ttcattatga tcaaaatcat ataattgttc cgttattatg cgaccatatg aatatatccg
     2101 aaaaaggata tgtgaaattt atgcgctaaa tgtttagtat tgttaaatat taagggtaaa
     2161 aggatgttta tcgcctggta ctggattgta ttgattgctc tggttgtggt gggttatttc
     2221 ctgcatttga aacgttattg tcgggcgttt cgccaggaca gagacgcact gcttgaagcg
     2281 cggaacaaat acttaaacag tacgagagaa gagacagccg aaaaggtaga ataaggcttt
     2341 cgtgaagctg gcagaagttg tccatgtgct ggacaacttc tgatgttgtt tttaaccaat
     2401 tcccgccgtt tgtagcagca ctaaactgaa tcccatcact gacattccac atagtacgcc
     2461 attgctgggg ttattattag ggtcaatctc tttggcgagc ggcattaatt catcaaccga
     2521 gagcgccacc ataattcctg caaccgccgc catgaccacc ggggaaatca tgctaccgag
     2581 gattaaccac gccagcacac caccaagaat ttctgctagt ccagaaatcc ccgcccacag
     2641 aatcgcggtg cgtttagacc ccgttgccgc ataaaccggg cctgccactg ccagaccttc
     2701 agggatattg tgcaacgcga cggccagtgc gatgccaaat cccagctcca ggttgctgct
     2761 cgccgtgaca aaggtggcaa tcccttccgg gaagttatgc agactgatgc cgagagtgag
     2821 cagaatggct gtgcgcttga tcgattttgg caacggctgc accgattttt gcattaaatc
     2881 ctgcggatgc gcatgcggca acatgcggtc caggccaaag tagccaagca gaccgaagat
     2941 aaacattcca taacccaaca caggcgacat tccttcagct gctagtgcgg caggaagcat
     3001 ttccattaat gagatgagca acatgatccc cgccgcaaaa cctagcgaaa acgccagtaa
     3061 gcggttcgag ggtttttgtc cgagaacgcc gagaaacgcg ccaataaacg tggctgcccc
     3121 cgccagtatg gtcagaatga gaggtactga catcgacaac tccttataaa tcttcacctt
     3181 caggcagatc gtcaaaataa ctgatgatca tcatcattgt tatccgggtt cttactccgc
     3241 cgaacaagcg tattgtgagg atatcaaaat gacaccctta gttaaggata tcatcatgtc
     3301 ttcaacacgt atgccagcat tgtttttagg tcacggtagt ccgatgaacg tgctggaaga
     3361 taatttgtat acccgcagct ggcagaagtt ggggatgaca ttgccacgcc cgcaagcgat
     3421 tgtggtggtt tcggctcact ggtttacccg tggaacagga gtgaccgcga tggagacgcc
     3481 gcccacgatt catgactttg gtggcttccc gcaggcgctg tacgatacgc attatcctgc
     3541 tccgggttcg cctgtgctgg cacagcatct ggttgagctg ttagcgccgg tcccggtgac
     3601 gctggataaa gaagcctggg gctttgacca cggttcctgg ggcgtgctga ttaagatgta
     3661 tcctgacgcc gatatcccga tggtgcagtt gagtatcgac agtagcaaac ctgccgcctg
     3721 gcatttcgaa atggggcgca aactggcagc gctgcgagat gaaggaataa tgttggtcgc
     3781 cagtggcaac gtggtgcata acctgcgtac agtgaagtgg cacggtgata gttcaccata
     3841 tccttgggcg atgtcgttta atgagtatgt gaaagcgaat ctgacgtggc aaggggcggt
     3901 agagcagcat cctctggtga attacctcga ccatgaaggt ggcgcgttat cgaacccaac
     3961 gccggagcac tatctgccgt tgttgtatgt cttaggtgcg tgggatgggc aggagccaat
     4021 taccattccg gtcgatggta tagaaatggg cagcctgagt atgctgtcgg tgcagatagg
     4081 ttaacaatta ccggatggcg aatgccatcc ggcatcattg attactcaac aaaaatatgt
     4141 ggataaaacc gagacatatc ctgggtgatc aatgcacggt cttcacgaat gccaattccg
     4201 gcgggttgat cgttcaccag ccagctacca atcagcatat agctgtcgcc gaatttcggt
     4261 aacggatgga attgctgaac aatcatccct tcttcgccat acggaccttc cgctgcttca
     4321 atggttttgc cgttctcaat gatcgacacg tttgcgcctt cacgggagaa gatcggttta
     4381 accacatatt tttccatttg cggatgatca tcttccgcaa aataagcggg cagcaggttc
     4441 gggtgattcg ggaacatctc ccacagtagc ggtagaagcg ctttgttgga gataatgctc
     4501 ttccacgccg gttccagcca gcgtacgcct gcatcctcca gcttggtgga gaacatctca
     4561 cgcaacataa attcccatgg atacagcttg aacaggttgg aaattacctg atcctgtaaa
     4621 tccgtgaact gacctttttc acctaacccg atatcgtcaa tgtagaggaa ctcggtagcg
     4681 atttcagctt ccgtcgcgca gtcctgcaaa tactgaatgg ttccgcgatc ttccaccgtg
     4741 tcgcgacagc aggtgagatg cagcaactgg aagccatact gttcacgcaa ctcgacaaag
     4801 cgatcgatta gcttttcttg cagactgtta aactggtcgc tgccctccgg caagttaccg
     4861 gcgttaagct gatcttccag ccagatccac tgaaagaacg ccgcctcgta tagtgacgtt
     4921 ggtgtatcgg cgttattttc cagcagttta ggttcgccag tgccatccca tgccagatca
     4981 agacgcgaat aaagcgatgg ctggtgagtc agccatgact gacgcacaaa actccaggtg
     5041 tgttttggaa tgcggaattt ggtcatcagc tcatcgctgg cgatcacttt ttccaccact
     5101 ttcaggcaca tctggtgcag ttcggcggtg acttcttcca gcttttcaac ctgggcgagg
     5161 gtcaacttgt agtaagcatc ttcacaccag tacggctcgc cgtacatggt gtgaaaattg
     5221 aaaccgtatt cgtgggcttt ttcacgccag tccgggcgct cggtaatact gactctttcc
     5281 atcggtatca gccacccatt gaacgagaag aggtgccggt tgcgctacgc tgcatagtgc
     5341 tttgtttggc aacagattca ccaaaaccgc cacgggtaac ggtagtagtg gtcgccggtt
     5401 ttggtgccat ggctgtcttc ggtacggtca tggtgcggcc tggctgggct gcgccatagt
     5461 ttttacccgt cgcgtcggta tatttaccgt aagccgggct ggctgggttt ttcgaggaga
     5521 acagcggctg ctgtgcaaat cccgcgccgc cgcccatcag acgccccatc atgtaaccgg
     5581 ccatcagcgg catccagaaa ctcccgctgg attgctgggc ctgcgcctgg ttttctggtg
     5641 ccatgccagc ctgggctggt gcctgctggc actgaccttc accaaattca gcaacacagt
     5701 cttcacgggt ggcgtatttc ggcgcagtac gttcggcttc tttcagcgca ttgttgtacg
     5761 cggtggtaca ttcggcgctt ttgcctgggt ttgcagctga acagtcgtca gcattttgat
     5821 agagagacac tgtttcatca ctcttttcac agctagccag cataaaaaca gtggcaaccg
     5881 cgagagcgac tggtgtcaga tggcgtgcgc tccagttttt gcggaacgat gcgtggcgta
     5941 tggattttgt ccgtttcatt tttgtcttcc gggaccagtg gtaaataccc atcagaatag
     6001 aggatggctg gtcgaaattg aagcgagaag ggggaagaat gcggcagata acccgtatct
     6061 ttacgttgtc ttacgttcag acggggccga agccccgtcg tcgtcatcag ttacggaaag
     6121 ggttatgacc gttactggtg gtagtgcgtg cggatgtttg ctgaacgacg ggtgccgggc
     6181 tatcaggcgc ataaccatca gcaatagcat tctgttccgg cgtttgcggg gcaacgtttt
     6241 ccggattagt ggaaaccggt ttgctcagcg cattgttcag tgccagcaga tcctgctcgt
     6301 tcaacgtacc cagggctgac ttaatattca gctgattaat caggtagtta taacgcgcat
     6361 ttgccagctc ttgcttagcg ttgtacagcg tggtggttgc atccaacaca tcaacaatgg
     6421 tacgcgtacc gaccgagtag cccgcttcca tcgcgtctaa tgagctttga gcggaaacta
     6481 cggcttgttt gtaggcgtta atgctactga tagatgcatt aatgttgttg aaggaggaac
     6541 gtacggtttg cacgatgcta cgatgcgcgc tttccagttg ctcgctggca ccaacaaagt
     6601 tgtactgggc ctgtttcacc tgcgagttaa ccattccgcc ctgataaatc ggcagcgaga
     6661 agctcaggcc cactttgttc tggcccatat tgctgtcgtc atactgggta ccagcggcac
     6721 cacgagtttt cgaaccgcta taagaggtgt cagaaatccc ggtagaagcc gttaaatcca
     6781 gcgtcggtaa gtgaccatcc tgcgcctggc gaatttgctc gcgcgccagg tcctggctca
     6841 agcgtgcctg taacagcgac aggttgcgtt tttcggcttc tttcagcagc gcgttaaccg
     6901 gctgtggttt gtcggtttta aagttttcga cattcagcgc cgccagttcc ggatagtagt
     6961 taccggtgat ctggcgcagc tgctctaccg cgttatcaag gttattacgt gcggtcactt
     7021 cgttcgccag cacggtatcg tactgcgcgc gggcgttctg cacgtcggtg atcgctacca
     7081 ggcccacgtt aaaacgttgg gtggtttgat ctaattgacg gtagatcgct tctttttgtg
     7141 cctgtgtata ggaaagaacg tcaatagcat tcaacacgtt gaaataagcg gtcgcggtgt
     7201 tgaggatcaa ggtttgctga tcggtctgat atgtgacgtc ctgaatccct gctgcttttt
     7261 cctgcagcgt taacgcacgc catttcgaca tatcaaaaat ggattgagtt aactgcaggg
     7321 acgcactggt cgcgttcgag ttgatgccgt tcgcgtcgcg gtagccgttg ctataggtgt
     7381 aatctgcacc taaacctagc tgtggcagta atggactgcg cgcttcatta attttttcaa
     7441 aggcagcatc acgatcggcg gcagacttac gcaattccgg gttactaagg cgtgcttgct
     7501 gataaacttg catcaggttc tcggcctggc tcaacgaact gaacccagaa aggctcaggc
     7561 cgataagaat ggggagcaat ttcttcattt gcattccttg tggtgaagca gtatttagcg
     7621 cgatcaaact gtaaaattat tgcggattct agcagaagcc gctaccgcaa aaggttggcg
     7681 ttacgtgcca tagggcgtta atgtgcctca atttaacaca tgactgctca aacggcagtc
     7741 aaacgtcgct gaaattcaca tttaattcac tattagtgcc aggacattaa caatgcttaa
     7801 gccagacaac ctgcccgtta catttggcaa aaacgatgta gaaattattg cacgagaaac
     7861 actttatcgc ggcttttttt cattagatct ttatagattt cgtcatcgtc tattcaacgg
     7921 gcaaatgagt catgaggtgc ggcgggaaat ttttgagcgc ggtcacgccg cagtcttgct
     7981 accctttgac ccagtgcgtg atgaagttgt gctgattgag cagattcgaa ttgccgcgta
     8041 cgacactagc gaaaccccct ggctactgga gatggttgcc gggatgattg aagagggtga
     8101 aagtgtggaa gatgttgccc gtcgcgaagc gattgaagag gcggggctga tagtcaaacg
     8161 gaccaaaccg gtgttaagtt tcctggcaag cccggggggc accagtgagc gttcgtcaat
     8221 tatggtgggc gaagtggacg ccacgaccgc aagcggtatt catggtctgg ctgatgaaaa
     8281 cgaagatatt cgcgttcatg tggtaagccg ggaacaggca taccagtggg tagaagaggg
     8341 gaaaatcgac aacgcagcgt cggtcatcgc tttgcaatgg ctgcagctgc accatcaagc
     8401 gttaaaaaat gagtgggcat aaatgaagcg ttacacacct gactttcctg aaatgatgcg
     8461 cctgtgcgag atgaactttt cacaattgcg ccgtttgtta ccgcgcaatg acgcacccgg
     8521 cgaaactgta agctatcagg tggcaaacgc acaatatcgg ctgacgattg tggaatcgac
     8581 ccgatacact accctggtga caatagaaca gactgcgccc gcgatcagtt actggagcct
     8641 tccgtcaatg acggtgcgtc tgtatcatga cgcgatggtg gctgaagtgt gttcaagcca
     8701 gcagattttt cgcttcaaag cgcggtatga ttatcctaat aaaaagttgc atcaacgcga
     8761 cgaaaagcat caaattaacc agtttttagc ggactggttg cgatactgtt tagcacatgg
     8821 agcgatggcg attccggttt actagcgtcg tgaaacctaa ggacaccatt tggaaagcct
     8881 gttaaccctt cctctggctg gtgaggccag agtcaggatt ttacaaatta ccgatactca
     8941 cctgtttgca caaaagcacg aagccctgtt aggggtaaac acctgggaaa gctaccaggc
     9001 tgtgctggag gcgattcgtc cacatcagca cgaattcgac ctgattgtcg cgacaggtga
     9061 tttagcgcag gatcaatcct ctgcggccta tcagcatttc gctgaaggca tcgcaagttt
     9121 tcgtgcgccc tgcgtctggc tgccgggcaa ccacgatttc cagcccgcga tgtacagcgc
     9181 gttacaggat gcgggtatct ccccggcgaa gcgcgtgttt attggtgagc aatggcaaat
     9241 cctgttgctg gatagccagg tgtttggcgt gccgcacggt gagctgagcg agtttcagct
     9301 tgagtggctg gaacgtaaac tggccgatgc gccagaacgc catacgttgc tgctgctgca
     9361 tcatcatccg ctacctgcgg gttgtagttg gctcgatcaa cacagtctgc gtaacgcggg
     9421 cgaactggat accgtgctgg cgaagtttcc gcacgtcaaa tacttgctgt gcggtcatat
     9481 tcatcaggag ctggatctcg actggaacgg tcgccgcctg ctggcaacgc cgtcgacctg
     9541 tgtgcagttt aagccgcact gttccaactt tacgctggat accatcgcgc ccggctggcg
     9601 tactctcgag ttacatgctg atggcacgct gaccaccgag gtgcatcgcc tggcggacac
     9661 acgtttccaa cctgataccg cttcagaagg ctactgatgt ctacgcttct ttatttacac
     9721 ggtttcaaca gctcgccgcg ctctgcaaaa gcgagcttgt taaaaaactg gctggcggaa
     9781 catcaccctg acgttgagat gatcattccg cagttgccgc cgtatccttc cgacgcggca
     9841 gagctgctgg aatccattgt cctggaacat ggcggcgatt cgctgggtat tgtcggttcg
     9901 tcactggggg gatattacgc cacctggttg tcacaatgtt ttatgctgcc cgcagtggtg
     9961 gtaaacccgg cggtgcgccc gtttgaactg ctgacggact atctcggtca gaacgagaac
    10021 ccctacaccg ggcagcaata tgtgctagag tcacgccata tttacgatct taaagtcatg
    10081 cagattgacc cgctggaagc gccggatttg atctggctgc tgcaacagac gggagatgaa
    10141 gtgctggatt accgccaggc ggtggcgtac tacgcttcct gccgccagac tgtcatagaa
    10201 ggcggcaacc acgcattcac gggcttcgaa gattatttca acccgatcgt cgattttctt
    10261 ggtctgcacc atctctgacg atcaactcga attactaact taaaccatga cgcaaactta
    10321 taacgctgat gccattgagg tactcaccgg gcttgagccg gttcgccgcc gtccggggat
    10381 gtataccgat accactcgcc ctaaccattt ggggcaagaa gtcattgata acagtgtgga
    10441 tgaagcactg gcgggtcacg caaaacgcgt ggacgttatt ttacatgctg accagtcgtt
    10501 agaagttatt gacgatgggc gcgggatgcc ggtggatatt cacccggaag agggtgtacc
    10561 ggcggttgaa ctgattcttt gccgtctgca tgcaggcggt aaattctcta acaaaaatta
    10621 ccagttctct ggcggcctgc atggcgtggg gatttcggtg gttaacgccc tgtcgaagcg
    10681 cgtagaagtt aacgtgcgcc gcgatggtca gatttataac atcgcctttg aaaatggcga
    10741 aaaggtgcag gatttacagg ttgtcggcac ttgcggtaaa cgcaataccg gcaccagcgt
    10801 ccatttctgg ccggatgaaa ccttcttcga tagcccgcgt ttttctgttt cacgcctgac
    10861 gcatgtgctg aaagccaaag cagtactgtg ccccggcgtt gagattacct ttaaagatga
    10921 gatcaacaac accgaacagc gctggtgcta tcaggacggt ctgaatgatt acctggcgga
    10981 agcggtaaac ggtttaccga cgctgccaga aaaaccgttt atcggtaatt tcgctggcga
    11041 tactgaagcg gtggactggg cgctactgtg gctgccggaa ggcggtgaac tgctgaccga
    11101 aagctacgtc aacctgatcc caacgatgca gggcggtacc catgttaatg gtctgcgtca
    11161 gggcctgctg gacgcgatgc gtgagttctg tgaataccgc aacattctgc cgcgcggtgt
    11221 aaagctgtcg gcggaagata tctgggatcg ctgcgcctat gtgctgtcag taaaaatgca
    11281 ggatccgcag tttgccgggc agaccaaaga gcgtctctct tcgcgtcagt gcgcggcatt
    11341 cgtttctggc gtggtgaaag atgcctttat cctgtggctg aaccagaacg ttcaggcggc
    11401 ggagttgctg gcagagatgg cgatttccag cgcccagcgt cgtatgcgtg cggctaaaaa
    11461 agtggtgcgt aaaaagctga ccagcggtcc ggcgctgcct ggcaaactgg ctgactgtac
    11521 cgcgcaggac cttaaccgca ccgaactgtt tcttgtggaa ggtgactccg caggcggatc
    11581 tgccaagcag gcgcgcgatc gcgaatatca ggcgatcatg ccactgaaag gtaagatcct
    11641 taacacctgg gaagtctctt ccgacgaagt gctggcttcg caggaagtgc acgatatttc
    11701 ggtagcgatc ggtatcgatc ctgacagcga cgatttgagc cagcttcgtt acggcaagat
    11761 ctgtatcctg gcggatgctg actccgatgg tctgcacatt gccacgctgc tctgcgcttt
    11821 gtttgtaaaa cacttccgcg cgttggtgaa acacggtcac gtttacgtcg cactgccacc
    11881 gctctaccgt attgatctcg ggaaagaggt ttattacgcg ctgacggaag aagagaaaga
    11941 gggcgtactt gagcaattaa aacgcaagaa aggcaagccg aacgtccagc gttttaaagg
    12001 tctgggggaa atgaacccga tgcaattgcg cgaaaccacg cttgatccga acactcgccg
    12061 tctgatgcag ttgactatcg atgatgaaga cgatcagcgt actgacgcga tgatggacat
    12121 gctactggcg aagaaacgct cggaagatcg ccgcaactgg ttgcaagaga aaggcgacat
    12181 ggcggagatt gaggtttaaa ggaaagaaca ttgcccggtc aggatggccg ggcaaacaag
    12241 gcaggattaa atccctggct gcagaatacg aatattcatc tccagcacgt caccttttac
    12301 ggcttcgctg tacgccttca tgtgcggggt ttgcagatgc gcttcaaggt gcgcgatgct
    12361 ttcccactgc tcaatcatca cgatagaatc cggtgccata gactggaaac tcacgccagc
    12421 agcgcaatcc accattggcg catagccgtg gcaaccttct tctttcagta cggttggaac
    12481 gattttagca aactgatcca ataccgcctg acggtgatgt tgaccaggac gagtacggat
    12541 ttctgcgatt acggtaagca tggttaactc cttctaaagc ctgagctcta gttaaccaaa
    12601 aatttccaca agatgcttgc gatattcttc agtatagcgg ggaacatcag gcatttttat
    12661 cacgtcatta gcgataaatg tcggcagcgg ttccatgcca aggaattggt ttgctttatg
    12721 gaacggcaga tacacaccgt cgacgccaac gccgtggaag aattgatctt tttcggtgaa
    12781 ggcttccatt ggtgcgttcc aggtcagaga aagcatatat tttttgccct gtaccaggcc
    12841 gccggaaccg tatttttttg acggatcttt gcgggtacgg ccatcgctgg catacagcgt
    12901 cccgtgacct tcggtgaata catcatcaat gtattttttc actgtccacg gcgcgcccat
    12961 ccaccagcct ggcatctgcc agatcaccac atcagcccag agaaagtttt gtacttccgc
    13021 tttgacatcg tagtcgctgt cggcgcgaac gatgcgaaca tcatgcccga ggtcgcgcag
    13081 tgtgccatcc gcgacttcgg tcagggtgtc attcagttga ccattggagt gggcgaattt
    13141 tttcgcgccg ttgataatca gaatgttgct cattttttaa cctcaaggtg agacgtttgc
    13201 cgactatagt acgtcacaga gcggtacggt gaaattagca aaatgtgcaa agtcttttgc
    13261 gaatttagca aaagtcttac ccttaccagc ttactttcgc ctcaaatcca ccttgttccg
    13321 cattcccaaa ctcaacattc atgccatgca gcttggcgat tcgctggaca atcgatagcc
    13381 caagtccact gccggtagcg gtttgtccgg gggggcgata gaagcgttcg ccaattcgcg
    13441 ccagtgcctc tggtgtcaca ccggggctgt tatccctcac gatgaaatta tcggcattca
    13501 gcgtgacgtc caccacgctg ccctgtggac tgtaacgcac ggcgttatcc agcaaatttc
    13561 gcaccaacaa acttagcaat agcggttgtc cggtgcgttt gatgccctgg acattgagtg
    13621 tcagtcgcac gtcaattttc gcctgctgcg ccgtgtggta aatatccatc accgacgatt
    13681 gcaggagatc ttcaagcggg atctccgcga cgtcctgaag gttatccagt gagtccagcc
    13741 gcgatagcgt gagcagttga tcaaccagac gagtagcgcg atcgatcccg gaatgtaatt
    13801 ggagtagtgc ttttttccgc gcctgcggat catcgtcaga gagctgcgca acttcggttt
    13861 gcactttcag cgctgttaac gggctacgta gttcgtgagc tgcatcggag gtaaagcgtc
    13921 gttctcgaac catcatcgca tgtgtgcggg cgaacagttg atttagcgac tcaaccagtg
    13981 gtcgcacttc gctgggtacg ccagtcgcgt ttagtggttt ttccgagtca gggtcacgca
    14041 tacgtagtgc cagcgccagt ttgttcagcg gcgcgagttc acgacccagt agtaccatca
    14101 tgatgattaa cataatcggc agtgcgacca gccacgggat caattgcccg gcaacaatcg
    14161 ccagcgccat gtcttcacgg tattcccatt cctggccaac aacgatgcga tatttgccat
    14221 caggtgaggt catccagaca aaacgccaag gatcgtcttc accgaccagt tgcccgtcag
    14281 caaaaccttc ccgttgatag ctatagggaa tatcttctcc gttatcgcca tcattaagga
    14341 ccattctgcc gtcgtgggta aagatggcaa aggtcagcgc atcgtcatca acatgaccgt
    14401 gttttaattt atttggcgtc tgtgccatgc gatccgccgc gttgatttcg ttgagatcga
    14461 gcgtacttaa ccgcttggca aacagcatca gttgggtgtc gaacaattca tcgacgttat
    14521 ccgttgtttg tttccaggcg acaaagctgg aaagcagcca ggtcaccgag gccagaatta
    14581 aaaagattag cgtcagcctg acgcgcagac taagacgttg ggtaaatttc atttctcacc
    14641 taatgtgtag ccaataccat gcacggtacg aataaaatca ctaccgagtt tgcgtcgcag
    14701 atgatgcaca tgcacttcaa cggcattact ggtgacctct tcgtcccagg tatacagttt
    14761 ctcttcaatc agtttgcgcg gcagtacccg accagcgtta cgcatcagta attccagcag
    14821 ggcaaattct tttggtttca gtgttaaggg ttcgccagcc agcgtggcga tacgtttgcc
    14881 ggggtcgagc atgacgttac cgtggcgcag ctcgttgctg gcctggccgt tggttcggcg
    14941 catcagagcc tccagcctgg cggcgacttc tatcaacgca aaaggtttac acagataatc
    15001 gtcagctccc agacgcagcc cttctacacg ttccgctaac gcatcacgcg cggtcaggat
    15061 cagtaccggc tcacgctgac ctttctctcg ccattcgcgc aaaatatcgc gaccatccat
    15121 gcctggtaag gttaaatcca ggatcaccgc atcataaggt gcgctataaa gcgcctcttt
    15181 tccctgacga ccttgtgtaa accagtcgac actaaaaccc attttactaa ggccagtttt
    15241 gatgccgtcg ccaatcagca tgtcatcttc tatcagtaaa attcgcattt tttcatccct
    15301 gcgataaccg tatttgctgc cagtaaaccg tgttgtaacg cgctctgtac agcattaaat
    15361 tttatttttt tcccttaaga agttgttaag gactatcttg ttaaatgctc ggaaacagac
    15421 attaaagggt gtaataaaca tgaaaaaatt cgcagcagta atcgcagtaa tggccctgtg
    15481 cagtgcaccg gtgatggcag cagagcaggg cggtttttct ggcccatcgg caacgcaaag
    15541 tcaggccgga ggattccaga ggccgaacgg cagcgtaacg actgtagaaa gcgcaaaatc
    15601 cctgcgtgac gacacctggg taaccctgcg cggcaatatc gttgaacgca tctctgacga
    15661 tctctacgtg tttaaagatg ccagcggtac tatcaatgtt gatatcgacc acaaacgctg
    15721 gaacggtgtg acggtgacgc cgaaagatac ggttgagatt cagggggaag tcgataaaga
    15781 ctggaattct gttgaaattg acgtcaaaca gatccgcaaa gtaaatccgt aattgttacc
    15841 gctcccggga cgcgttcccg ggaataattt cgcagggagg caaaatgaca aacctgacac
    15901 tggatgtaaa cattatcgat ttcccatcaa tccctgtggc gatgttgccg caccgctgta
    15961 gccctgagtt gctcaactac agtgtggcga aatttatcat gtggcgtaaa gagacggggc
    16021 tttctcctgt taaccaaagc cagacttttg gcgtcgcctg ggacgaccct gccaccaccg
    16081 caccgaaagc gtttcgcttt gatatctgcg gcagcgttag cgaaccgatt cccgataatc
    16141 gttatggtgt gagcaatggt gaacttaccg gtggacgtta tgccgtggcc cgccacgttg
    16201 gcgagctgga cgatatttca cacacggtat ggggcatcat tcgccactgg ctgcctgcaa
    16261 gcggcgagaa aatgcgtaaa gcaccgattc tgtttcacta caccaatctt gccgaaggta
    16321 tgacagaaca gcgactggaa acggatgttt atgtgccgtt agcgtgatgg ggaatcttta
    16381 ccggagtgtg agccaaatca ctcgcgataa gtttgagcga acaatgccac aggcgtattg
    16441 ttgttgggct tataatttct ccggtaagga ataccatgta tacgcgaaat ttattatccg
    16501 gtgcgccgta agccgtctcg ggggtatcca tgagcaagcc gcacttatgg caggaggcgc
    16561 ggggcactgg tggagacaca gctaaactgg gctgaaccgt atacttacag tcgactactt
    16621 tgatgcttta ggcttccctg gactcgcata acctcaactt ctcgaatcgc ccggagcgga
    16681 cccccatgcc gagtggtgtg gcatgggcgc aaccttgtgc tgccctctat gccgatcaag
    16741 tctacagcaa agagttgtat atcaaggcac attgatgcct ttatcgccgt cggagcacgt
    16801 ccggcggcgc tattcacgct gatctcctgt gactcgacgc ggcagataat gtagtatctc
    16861 cggcaatatt gcccctttga aggctggcga ataagttgag gaatcagaat taatgagcga
    16921 tatggcagag cgccttgcgc tacatgaatt tacggaaaac gcctacttaa actactccat
    16981 gtacgtgatc atggaccgtg cgttgccgtt tattggcgat ggtcttaaac ctgttcagcg
    17041 ccgcattgtg tatgcgatgt ctgaactggg cctgaatgcc agcgccaaat ttaaaaaatc
    17101 ggcccgtacc gtcggtgacg tactgggtaa ataccatccg cacggcgata gcgcctgtta
    17161 tgaagcgatg gtcctgatgg cgcagccgtt ctcttaccgt tatccgctgg ttgatggtca
    17221 ggggaactgg ggcgcgccgg acgatccgaa atcgttcgcg gcaatgcgtt acaccgaatc
    17281 ccggttgtcg aaatattccg agctgctatt gagcgagctg gggcagggga cggctgactg
    17341 ggtgccaaac ttcgacggca ctttgcagga gccgaaaatg ctgcctgccc gtctgccaaa
    17401 cattttgctt aacggcacca ccggtattgc cgtcggcatg gcgaccgata ttccaccgca
    17461 taacctgcgt gaagtagctc aggcggcaat cgcattaatc gaccagccga aaaccacgct
    17521 cgatcagctg ctggatatcg tgcaggggcc ggattatccg actgaagcgg aaattatcac
    17581 ttcgcgcgcc gagatccgta aaatctacga gaacggacgt ggttcagtgc gtatgcgcgc
    17641 ggtgtggaag aaagaagatg gcgcggtggt tatcagcgca ttgccgcatc aggtttcagg
    17701 tgcgcgcgta ctggagcaaa ttgctgcgca aatgcgcaac aaaaagctgc cgatggttga
    17761 cgatctgcgc gatgaatctg accacgagaa cccgacccgt ctggtgattg tgccgcgttc
    17821 caaccgcgtg gatatggatc aggtgatgaa ccacctcttc gctaccaccg atctggaaaa
    17881 gagctatcgc attaacctca atatgatcgg tctggatggt cgtccggcgg tgaaaaacct
    17941 gctggaaatc ctctccgaat ggctggtgtt ccgtcgcgat accgtgcgcc gccgactgaa
    18001 ctatcgtctg gagaaagtcc tcaagcgcct gcatatcctg gaaggtttgc tggtggcgtt
    18061 tctcaacatc gacgaagtga ttgagatcat tcgtaacgaa gatgaaccga aaccggcgct
    18121 gatgtcgcgg tttggcctta cggaaaccca ggcggaagcg atcctcgaac tgaaactgcg
    18181 tcatcttgcc aaactggaag agatgaagat tcgcggtgag cagagtgagc tggaaaaaga
    18241 gcgcgaccag ttgcagggca ttttggtttc cgagcgtaaa atgaataacc tgctgaagaa
    18301 agaactgcag gcagacgcgc aagcctacgg tgacgatcgt cgttcgccgt tgcaggaacg
    18361 cgaagaagcg aaagcgatga gcgagcacga catgctgccg tctgaacctg tcaccattgt
    18421 gctgtcgcag atgggctggg tacgcagcgc taaaggccat gatatcgacg cgccgggcct
    18481 gaattataaa gcgggtgata gcttcaaagc ggcggtgaaa ggtaagagca accaaccggt
    18541 agtgtttgtt gattccaccg gtcgtagcta tgccatcgac ccgattacgc tgccgtcggc
    18601 gcgtggtcag ggcgaaccac tcaccggcaa attaacgttg ccgcctgggg cgaccgttga
    18661 ccatatgctg atggaaagcg acgatcagaa actgctgatg gcttccgatg cgggttacgg
    18721 tttcgtctgc acctttaacg atctggtggc gcgtaaccgt gcaggtaagg ctttgatcac
    18781 cttaccggaa aatgcccatg ttatgccgcc ggtggtgatt gaagatgctt ccgatatgct
    18841 gctggcaatc actcaggcag gccgtatgtt gatgttcccg gtaagcgatc tgccgcagct
    18901 gtcgaagggc aaaggcaaca agattatcaa cattccatcg gcagaagccg cgcgtggaga
    18961 agatggtctg gcgcaattgt acgttctgcc gccgcaaagc acgctgacca ttcatgttgg
    19021 gaaacgcaaa attaaactgc gcccggaaga gttacagaaa gtcactggcg aacgtggacg
    19081 ccgcggtacg ttgatgcgcg gtttgcagcg tatcgatcgt gttgagatcg actctcctcg
    19141 ccgtgccagc agcggtgata gcgaagagta atatgccggg gagggcctgt ttcctcccgc
    19201 ttgcaaggaa cgccggatga aacgttaagt cgtcatccgg cagcgtattc aacccccgga
    19261 aacgataaaa attcccttga aaccgttgtt tattcatgcg ttgcgattaa caatacgctt
    19321 ttccagagag cggcttttaa caatgcccta acctgatttc aggtgacgta caatgccagt
    19381 ctctcagacc ttcagaggtt gttatgctat atatctttcg tcttattatt accgtgattt
    19441 acagcatctt agtctgtgta ttcggctcca tttactgcct tttcagcccg cgtaacccga
    19501 aacatgtggc cacctttggg catatgtttg gccgtcttgc gccgctgttc ggcctgaaag
    19561 ttgagtgccg caaacctgca gatgctgaaa gctacggcaa tgctatctat atcgctaacc
    19621 accagaacaa ctatgacatg gtgacagcat cgaacattgt gcaaccgccg acggtgacgg
    19681 taggtaaaaa gagcttgctg tggatcccct tcttcgggca gttgtactgg ttaaccggca
    19741 acttattgat cgacagaaac aatcgcacta aagctcacgg caccattgcg gaagtcgtga
    19801 atcatttcaa aaaacgccgt atttccatct ggatgttccc ggaaggaacc cgcagccgtg
    19861 gtcgcggcct gctaccgttc aagactggag catttcacgc ggcaattgcg gcgggcgtcc
    19921 cgattattcc cgtgtgcgtc tctacaactt cgaataagat taatcttaat cgactgcaca
    19981 acggtctggt gattgtcgaa atgctgccgc caattgacgt cagtcagtat ggaaaagatc
    20041 aggttcgtga gctggctgcc cattgtcgtt cgataatgga acaaaaaatc gccgagctcg
    20101 ataaagaagt cgcagaacgc gaagccgccg gaaaagttta agtcggcaat ctgtattttt
    20161 gcggggaaca ctttcctgca cggtattact ttacccagtt ttacatggag caaatatgtc
    20221 actcagtcgg cgtcagttca ttcaggcatc ggggattgca ctttgtgccg gcgctgttcc
    20281 cctgaaggcc agcgcagccg ggcaacagca accgctaccc gttccgccgc tacttgaatc
    20341 tcgccgtggg caaccgctgt ttatgactgt acaacgtgcg cactggtcat ttacgccagg
    20401 gacacgcgcg tcggtctggg gaatcaatgg tcgttacctg gggccgacta tccgcgtctg
    20461 gaagggcgac gatgttaagc ttatttacag caaccgcctg atagaaaatg tctcaatgac
    20521 ggtggccggg ctacaggtac caggcccgct gatgggcggt ccggcacgga tgatgtcgcc
    20581 aaacgctgac tgggcacccg tactgcccat tcgccagaac gcagctactc tgtggtatca
    20641 cgccaatact cccaaccgca cggctcagca ggtctataac ggccttgccg gaatgtggct
    20701 ggtggaagat gaagtcagca agtcgctgcc tatccccaac cattatggtg tggatgattt
    20761 tccggtcatt atccaggata aacggctgga taactttggt acgccagaat acaacgaacc
    20821 gggaagcggc ggctttgttg gtgatacgct gctggttaac ggtgtacaaa gcccgtacgt
    20881 tgaagtctcg cgtggctggg tgcgcttgcg actgctgaac gcgtcgaact ctcgtcgcta
    20941 tcaactacag atgagcgatg gtcgcccgtt acatgtgatt tctggcgatc agggattcct
    21001 gcctgctcct gtatcggtga agcaactttc gttggcaccg ggcgagcgcc gcgagattct
    21061 ggtggatatg agcaacggtg atgaagtgtc gatcacctgt ggcgaagcgg cgagcattgt
    21121 tgatcgtatt cgtggcttct ttgagccatc cagcattctg gtttctaccc tggtgctaac
    21181 gctgcgccca accggccttc tgccgctggt cacagacagt cttccgatgc gcttgctgcc
    21241 aactgaaatc atggccggtt cgccgattcg cagtcgcgat atcagtctgg gtgatgaccc
    21301 gggtattaat ggacaactgt gggacgtcaa ccgtattgat gtcaccgcgc agcaaggaac
    21361 gtgggaacgc tggacggtac gcgcggacga gccgcaagcg ttccatattg aaggcgtgat
    21421 gttccagatc cgtaacgtga atggtgcgat gccgttcccg gaagacagag gctggaaaga
    21481 taccgtttgg gttgacggac aagtggagct gcttgtttat ttcggtcagc cttcctgggc
    21541 gcacttcccg ttctacttca acagtcagac gctggaaatg gcggaccgtg gctcgattgg
    21601 gcaactgtta gtcaatccgg taccgtaatc ttcatactcg cccgaggagg gcgagtatgg
    21661 aatacttttc gatttctctt tcgttccggc ttataatccc cggcctttga ttattttttt
    21721 atcatttctg gaagcaaaat gagctctatc tccctgatcc aaccggatcg cgacctgttc
    21781 tcctggccgc agtactgggc cgcctgtttt ggaccggcac cgtttttgcc gatgtctcgt
    21841 gaagagatgg atcaacttgg ctgggatagc tgcgacatca ttttggttac tggcgacgcg
    21901 tatgtcgatc acccaagctt cgggatggcg atttgcggtc gtatgctgga agcgcagggc
    21961 tttcgcgtcg ggatcatcgc ccagccggac tggagcagca aagacgactt tatgcgtctg
    22021 ggtaaaccga atctgttttt cggtgttact gctggcaaca tggattcgat gatcaaccgt
    22081 tataccgccg atcgccgttt acgtcatgac gatgcctaca cgccggataa cgtcgcgggt
    22141 aagcgcccgg atcgcgccac actggtttat acccagcgtt gtaaagaggc gtggaaagat
    22201 gtaccggtga tcctcggcgg tattgaggct agtctgcgtc gtaccgcaca ttatgattac
    22261 tggtccgata ccgtgcgccg ttccgtgctg gtggattcga aagccgacat gctgatgttt
    22321 ggtaacggtg agcgtccgct ggtggaggtg gcgcaccgtc tggcgatggg cgagccgatt
    22381 agtgaaatcc gcgatgtgcg taataccgcg attatcgtga aagaggccct gcctggctgg
    22441 agcggcgtgg attccacccg tcttgatacc cctggaaaaa tcgacccaat cccgcatccg
    22501 tatggtgaag atttgccgtg cgcggataac aaaccggtgg caccgaaaaa gcaggaagcc
    22561 aaagccgtaa ccgtgcagcc accgcgcccg aaactgtggg aaaaaaccta cgtgttgctg
    22621 ccttctttcg agaaagtgaa gggcgataaa gtgctgtacg cccatgcttc gcgtattctg
    22681 caccacgaaa ccaacccagg ctgtgcccgc gcattgatgc aaaaacacgg cgaccgctat
    22741 gtgtggatca acccgcctgc tattccgctt tctaccgaag agatggacag cgtttttgcg
    22801 ctgccataca agcgcgtgcc acatccggcc tatggcaatg cccgtattcc ggcttacgaa
    22861 atgatccgtt tttcggtcaa cattatgcgt ggctgctttg gcggctgctc tttctgttct
    22921 atcaccgagc acgaagggcg cattattcag agccgttccg aagattcgat cattaatgag
    22981 atcgaagcga tccgcgacac cgttccaggt tttacgggcg taatttccga tctcggtggg
    23041 ccaactgcca acatgtatat gttgcgctgc aaatcgccac gcgctgagca aacctgccgc
    23101 cgtttgtcgt gcgtttatcc ggatatttgt ccgcacatgg acacgaacca acgatcaacc
    23161 tctatcgccg tgcgcgtgat ctgaaaggca ttaaaaagat cctgattgcc tctggtgtgc
    23221 gttatgacat cgccgtggaa gatccgcgct atatcaaaga actggcgacc catcacgtcg
    23281 gcggttatct gaagattgcc ccggaacata ccgaagaagg gccgttatcg aagatgatga
    23341 agccgggcat gggcagctat gaccgcttta aagagctgtt cgatacttac tcaaaacagg
    23401 caggtaaaga gcagtatctg atcccctatt tcatctccgc gcaccccggt acgcgtgatg
    23461 aagatatggt gaatctggcg ctgtggctga aaaagcatcg tttccgtctc gaccaggtac
    23521 agaacttcta cccatcgccg ctggcgaact caaccaccat gtattacacc gggaaaaact
    23581 cgctggcgaa gattggttat aagagtgaag acgtcttcgt accgaagggc gacaagcagc
    23641 gtcgtttgca taaagcgttg ttgcgttacc acgatccggc aaactggccg ttaatccgcc
    23701 aggcgctgga agcgatgggt aaaaagcatc tgattggtag ccgtcgcgat tgcttagtgc
    23761 ctgcgccaac cattgaagag atgcgtgaag ctcgtcgcca gaaccgcaat acccgtccgg
    23821 cgttgacgaa acatacgccg atggcgaccc agcgtcagac gcctgctacg gcaaaaaaaa
    23881 gcgtcgtcta cgcaatctcg tccggtgaat gctggtgcga agaaacggcc taaagcggcg
    23941 gttggacgtt aaattcgctg ttcccggtag cagaattgct accggggata ttacatcaga
    24001 atctatctca ttaggcattt atttatatgc cga
//
LOCUS       DAJTNA010000048        24006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTNA010000048 DAJTNA010000000
VERSION     DAJTNA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24006
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.049"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(87..1037)
                     /locus_tag="OO161_001605"
                     /pseudo
     CDS             complement(87..1037)
                     /locus_tag="OO161_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            complement(1061..1351)
                     /locus_tag="OO161_001606"
     CDS             complement(1061..1351)
                     /locus_tag="OO161_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5462725.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            complement(1610..2491)
                     /gene="yddG"
                     /locus_tag="OO161_001607"
     CDS             complement(1610..2491)
                     /gene="yddG"
                     /locus_tag="OO161_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5462726.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            2723..5770
                     /gene="fdnG"
                     /locus_tag="OO161_001608"
     CDS             2723..5770
                     /gene="fdnG"
                     /locus_tag="OO161_001608"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3308..3310,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5462727.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFVPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            5783..6667
                     /gene="fdnH"
                     /locus_tag="OO161_001609"
     CDS             5783..6667
                     /gene="fdnH"
                     /locus_tag="OO161_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5462728.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            6660..7313
                     /gene="fdnI"
                     /locus_tag="OO161_001610"
     CDS             6660..7313
                     /gene="fdnI"
                     /locus_tag="OO161_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5462729.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(7364..7648)
                     /locus_tag="OO161_001611"
     CDS             complement(7364..7648)
                     /locus_tag="OO161_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5462730.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(7794..8804)
                     /gene="adhP"
                     /locus_tag="OO161_001612"
                     /pseudo
     CDS             complement(7794..8804)
                     /gene="adhP"
                     /locus_tag="OO161_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(8938..10635)
                     /gene="maeA"
                     /locus_tag="OO161_001613"
     CDS             complement(8938..10635)
                     /gene="maeA"
                     /locus_tag="OO161_001613"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5462731.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNDEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(10792..10929)
                     /gene="sra"
                     /locus_tag="OO161_001614"
     CDS             complement(10792..10929)
                     /gene="sra"
                     /locus_tag="OO161_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR5462732.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(11031..11246)
                     /gene="bdm"
                     /locus_tag="OO161_001615"
     CDS             complement(11031..11246)
                     /gene="bdm"
                     /locus_tag="OO161_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR5462733.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            11592..12023
                     /gene="osmC"
                     /locus_tag="OO161_001616"
     CDS             11592..12023
                     /gene="osmC"
                     /locus_tag="OO161_001616"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5462734.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(12079..13149)
                     /locus_tag="OO161_001617"
     CDS             complement(12079..13149)
                     /locus_tag="OO161_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5462735.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMRALAEELAVQMGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFFYERCPLATSG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(13146..14042)
                     /locus_tag="OO161_001618"
     CDS             complement(13146..14042)
                     /locus_tag="OO161_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5462736.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIALML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAVHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(14039..14411)
                     /locus_tag="OO161_001619"
                     /pseudo
     CDS             complement(14039..14411)
                     /locus_tag="OO161_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(14413..15963)
                     /locus_tag="OO161_001620"
     CDS             complement(14413..15963)
                     /locus_tag="OO161_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5462737.1"
                     /translation="MKRSIPFRPTLLALVLATTFSVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWSFTLKDN
                     AKFADGTPVTAEAVKLSFERILKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGAYIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(16049..16558)
                     /gene="ddpX"
                     /locus_tag="OO161_001621"
     CDS             complement(16049..16558)
                     /gene="ddpX"
                     /locus_tag="OO161_001621"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR5462738.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVVYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDKHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELP"
     gene            complement(16778..19012)
                     /gene="dosP"
                     /locus_tag="OO161_001622"
                     /gene_synonym="pdeO"
     CDS             complement(16778..19012)
                     /gene="dosP"
                     /locus_tag="OO161_001622"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR5462739.1"
                     /translation="MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMF
                     FNPAAEKLWGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLE
                     KKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVL
                     DPERHIVQCNRAFTEMFGYCMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKT
                     ARDQDEFLLLTRTGEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILA
                     AMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSA
                     TIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFD
                     PMTGLPNRNNLHNYLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRF
                     RGKLKPDQYLCRIEGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSI
                     GISYDVGKNRDYLLSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAIS
                     NNQLKLVYQPQIFAETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWV
                     IAEACRQLAEWRSQNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEIT
                     ESMMMEHDTEIFKRIQILRDMGVGLSFLFPPPTRRRNSRLDEQRVTAENLTNSSRPHS
                     RVFI"
     gene            complement(19013..20394)
                     /gene="dosC"
                     /locus_tag="OO161_001623"
                     /pseudo
     CDS             complement(19013..20394)
                     /gene="dosC"
                     /locus_tag="OO161_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(20766..22085)
                     /locus_tag="OO161_001624"
     CDS             complement(20766..22085)
                     /locus_tag="OO161_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5462740.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(22216..23751)
                     /gene="gadC"
                     /locus_tag="OO161_001625"
     CDS             complement(22216..23751)
                     /gene="gadC"
                     /locus_tag="OO161_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5462741.1"
                     /translation="MATSVQTGRAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         6046 a         5891 c         5962 g         6107 t
ORIGIN      
        1 ttaagtgctg gggaaaaaaa attaaataac atgagctgca tagtagggca attgttcttc
       61 ccagaaacat ttaaaaaatt taagtttttg ctctgtttta ttatggggaa taaagttcca
      121 atatcggatg tattattagt attaatgtgg gttattttat tattgtccag tcttaaagtt
      181 ttgatagatg gaagataagt caggaaatca agatttatat atttcaactg gttgttagca
      241 gcgtttaatg tctgaacatt gcagaagttg ctatagaata ttttaatttc agaaagcaaa
      301 ttgttgttaa taagtaacgt attcaagttg ggaaataagt cattgtgtaa acttgttagt
      361 ttattatggg atagatccag cagtaagagt tcatttttat tacctgcaac aatatcagtt
      421 aattgattat ggctgagatt caggtattgc agccattcgc aagattctaa ttgcacaaac
      481 tctagttgat tatgtgccgc actaaaataa gtaactgatg aaagcctatc tatatcaata
      541 tttctgagag cattattatt catagatata tgtgtaatac ttacatttct gccttgagga
      601 aaatttatag actcaagagc attactattc agacttagct ttacgagtct atcgtagttg
      661 gttgcatcaa aatgtgtgag ttggttatta tcaagattga tagaggtgaa gttttgcagc
      721 cctggtggtt gagtggttaa attaagtcct gataaatcaa tggtcacttc attagtgtca
      781 tcaattatat ttcttactgc agtccagtac tgcatttgtg caagtggatc attgttgatg
      841 ccgttaacta atacatgcca aagatgtttt gatgatatta gttgatcaat gtcattggtt
      901 ggcagtaaat tgttattgct taagtttaaa tattccagtt ttgtagtgtt tttggcgttt
      961 aaatgtgcaa tatggttgtc gtttaaagtg attgttttca tcctgggatg attgtgtaat
     1021 ataaggtcag tgatcatatt gtgatgggca cttaactcaa tcagagattc ttgccgcgcg
     1081 agccgttggc ttcatcgtta tagcctttaa aatcatattc ccagtgacca aaaccaatca
     1141 tttgatcatt gatttgcgtt tctcctttaa agccgagacg ggcataagtt ttatcaccat
     1201 cgtcacgctt atcatcagta aaataacgta gagcggtaac cttgccataa aggtcgagtt
     1261 tattaccatc cttgttatag acttcggcag cgtgtgctac gttcgcagct aacaaaccag
     1321 ttacaaccac cgcaactatt tttaatttca tttaacagaa tccttttaat tatcgttaaa
     1381 cgtattttct aaacgaattt taaacggcgt catttataaa tgacatactg tttttaaaat
     1441 agaatggtga ttaaaaacaa tgagaaaaat agatgaaata atattattta tcgatatgtg
     1501 atcgaagtcg aaatgagata taaggtgaat tactggtatt tgaaatttat tttttaatat
     1561 tgtcggaatt tatctgatta actgccgggc cgtagacccg gcgattattt taaccacgac
     1621 gtgtcgccag ccagcagagc agggaaccgc cgcagaccat tagcgcgcct tgccagaatg
     1681 agaacgacag cggggcgctg agcagcacgg ctgcaagcgc tgaggaaagt acgggcgtaa
     1741 aatacgagcc taccgccatt atggtgacat tgccatgcaa tataccgaca ttccatgcag
     1801 cataagcaaa tcctaaggta aatgccgcag agatgagttt aatcatgacg ggcgtgctaa
     1861 ataccatttc tggttgttgc gtaagaaaat agtaaaccca cagacttgct cccgttagta
     1921 ggacaaaaac ggtaattccg ttaaatccgc gtgcgtattt attcgttact gtgcaatagg
     1981 ctgcccagat aaacgcacca ataaacgcca ggaaataact caatgggctg gtggtgatat
     2041 tattgatgat ttcatcataa tgtaacccat tctcaccgcc taacacccag cagacgccga
     2101 ccagggctaa taataatcct ggtacaatca gccagttggt tttctgacca ttaaacagaa
     2161 tggcaaagag aattgtcagg ctgggccaca gatagttcac catacccact tcaatcgcct
     2221 gatgacgggt cgccgcatac cctaaggaaa gcgccagaca gatttcatag ctgacgaata
     2281 acagactccc ggcgagtaaa tagcctttcg gaatctgccg aatacgcgga aatccaaccg
     2341 tgaagattaa cagcagcccg cttaatgaat agatagcagc tgcgccgccg accgggccga
     2401 gcccctcgct gacaccgcga atcaatccta ccatcgtgct ccacaggacg atcgctatta
     2461 gccctatgag cgttgctttt tgtcgtgtca tgctcgctgt tttgtctctc ttgccgttaa
     2521 aaattaagct gaattttata gcattttttt aactggcctg tcaggcagtg gtgcgttttt
     2581 ctaccgctat tgaggcaggt caatttgcgc aggcggatta ttttgtggca aacacgtgtt
     2641 ctttttgatt tcgcgcaaaa agattcagaa ttttactgtt agtttcctcg cgcagtaata
     2701 cccctgaaaa aagaggaaag caatggacgt cagtcgcaga caatttttta aaatctgcgc
     2761 gggcggtatg gctggaacaa cggtagcggc attgggcttt gtccctaagc aagcactggc
     2821 tcaggcgcga aactacaaat tattacgcgc taaagagatc cgtaacacct gcacatactg
     2881 ttccgtaggt tgcgggctat tgatgtatag cctgggtgat ggcgcgaaaa acgccagaga
     2941 agcgatttat cacattgaag gtgacccgga tcatccggta agccgtggtg cgctgtgccc
     3001 aaaaggggcc ggtttgctgg attacgtcaa cagcgaaaac cgtctgcgct acccggaata
     3061 tcgtgcgcca ggttctgaca aatggcagcg cattagctgg gaagaagcat tctcccgtat
     3121 tgcaaagctg atgaaagctg accgtgacgc taactttatt gaaaagaacg agcagggcgt
     3181 aacggtaaac cgttggcttt ctaccggtat gctgtgtgcc tccggtgcca gcaacgaaac
     3241 cgggatgctg acacagaaat ttgcccgctc cctcgggatg ctggcggtag acaaccaggc
     3301 gcgcgtctga cacggaccaa cggtagcaag tcttgctcca acatttggtc gcggtgcgat
     3361 gaccaaccac tgggtggata tcaaaaacgc taacgtcgta atggtaatgg gcggtaacgc
     3421 tgctgaagcg catcccgtcg gtttccgctg ggcgatggaa gcgaaaaaca acaacgatgc
     3481 aaccttgatc gctgtcgatc ctcgttttac gcgtaccgct tctgtggcgg atatttacgc
     3541 acctattcgt tccggtacgg acattacgtt cctgtctggc gttttgcgct acctgatcga
     3601 aaacaacaaa atcaacgccg aatacgttaa acattacacc aacgccagcc tgctggtgcg
     3661 tgatgatttt gctttcgaag acggcctgtt cagcggctac gacgctgaaa aacgccagta
     3721 tgataagtcg tcctggaact atcagttcga tgaaaacggc tatgcaaaac gcgatgaaac
     3781 actgactcat ccgcgctgtg tgtggaacct gctgaaagcg cacgtttccc gctacacgcc
     3841 ggacgtcgtt gaaaacatct gcggtacgcc aaaagccgac ttcctgaaag tgtgtgaagt
     3901 gctggcctcc accagcgcac cggatcgcac aaccaccttc ctgtacgcgc tgggctggac
     3961 gcagcacacc gtgggtgcgc agaacatccg tactatggcg atgatccagt tgctgctcgg
     4021 taacatgggt atggccggtg gcggcgtgaa cgcattgcgt ggtcactcca acattcaggg
     4081 gctgactgac ttaggtctgc tctctaccag cctgccaggt tatctgacgc ttccgtcaga
     4141 aaaacaggtt gatttgcagt cgtatctgga agcgaacacg ccgaaagcga cgctggctgg
     4201 tcaggtgaac tactggagca actatccgaa gttcttcgtt agcctgatga aatctttcta
     4261 tggcgatgcc gcgcagaaag agaacaactg gggctatgac tggctgccga agtgggacca
     4321 gacctacgac gtcatcaagt atttcaacat gatggacgaa ggcaaagtca ccggttattt
     4381 ctgccagggc tttaacccgg ttgcgtcctt cccggacaaa aacaaagtgg tgagctgcct
     4441 gagcaagctg aagtacatgg tggttatcga tccgctggtg actgaaacct ctaccttctg
     4501 gcagaaccac ggcgagtcga acgatgtcga tccggcgtct attcagactg aagtattccg
     4561 tctgccttcg acctgctttg ccgaagaaga tggttctatc gctaactccg gtcgctggtt
     4621 gcagtggcac tggaaaggtc aggacgcgcc gggcgaagcg cgtaacgacg gcgaaattct
     4681 ggcgggtatc taccatcatc tgcgcgagct gtaccaggcc gaaggtggta aaggcgtaga
     4741 accgctgatg aagatgagct ggaactacaa gcagccgcac gaaccgcaat ctgacgaagt
     4801 ggctaaagag aacaacggct acgcgctgga agatctctat gacgctaatg gcgtgcttat
     4861 tgcgaagaaa ggtcagttgc tgagtagctt tgcgcatctg cgtgatgacg gtacaaccgc
     4921 atcttcttgc tggatctaca ccggtagctg gacagagcag ggcaaccaga tggctaaccg
     4981 cgataactcc gacccgtccg gtctggggaa tacgctggga tgggcctggg cgtggccgct
     5041 caaccgtcgc gtgctgtaca accgtgcttc ggcggatatc aacggtaaac cgtgggatcc
     5101 gaaacggatg ctgatccagt ggaacggcag caagtggacg ggtaacgata ttcctgactt
     5161 cggcaatgcc gcaccgggta cgccaaccgg gccgtttatc atgcagccgg aagggatggg
     5221 acgcctgttt gccatcaaca aaatggcgga aggtccgttc ccggaacact acgagccgat
     5281 tgaaacgccg ctgggcacta acccgctgca tccgaacgtg gtgtctaacc cggttgttcg
     5341 tctgtatgaa caagacgcgc tgcggatggg taaaaaagag cagttcccgt atgtgggtac
     5401 gacctatcgt ctgaccgagc acttccacac ctggaccaag cacgcattgc tcaacgcaat
     5461 tgctcagccg gaacagtttg tggaaatcag cgaaacgctg gcggcggcga aaggcattaa
     5521 taatggcgat cgtgtcactg tctccagcaa gcgtggcttt atccgcgcgg tggctgtggt
     5581 aacgcgtcgt ctgaaaccgc tgaatgtaaa tggtcagcag gttgaaacgg tgggtattcc
     5641 aatccactgg ggctttgagg gtgtcgcgcg taaaggttat atcgctaaca ctctgacgcc
     5701 gaatgtcggt gatgcaaact cgcaaacgcc ggaatataaa gcgttcttag tcaacatcga
     5761 gaaggcgtaa gggggcgaac agatggctat ggaaacgcag gacattatca aaaggtccgc
     5821 aactaactcc atcacgccgc cttctcaggt gcgtgattac aaagcagaag tcgcaaaact
     5881 tatcgacgtt tccacctgta tcggctgtaa agcctgtcag gtggcgtgtt cggagtggaa
     5941 cgacatccgt gatgaagtgg ggcactgcgt cggggtttac gataaccccg ccgatctgag
     6001 cgccaagtcc tggacggtga tgcgctttag cgaaaccgaa cagaacggca agctggagtg
     6061 gctgatccgt aaagacggct gtatgcactg tgaagatcct ggctgcctga aggcgtgccc
     6121 gtctgctggt gcaatcattc agtacgctaa cgggattgtc gatttccagt cggaaaactg
     6181 catcggctgt ggttactgca ttgccgggtg tccgtttaat attccgcgcc tcaacaaaga
     6241 ggataaccgg gtatataaat gcacgctctg cgtcgatcgc gtcagcgtcg gccaggaacc
     6301 ggcttgtgtg aaaacctgtc cgaccggggc tatccacttc ggcaccaaga aggagatgct
     6361 ggagctggcg gaacagcgcg tggcgaaact gaaagcgcgt ggttacgaac atgctggcgt
     6421 ctacaacccg gaaggggtcg gtggtacgca cgttatgtac gtgctgcatc acgccgatca
     6481 gccggagctg tatcacggtc tgccgaaaga tccgaagatc gacacctcgg taagcctgtg
     6541 gaaaggcgcg ttgaaaccgc tggcagcggc tggctttatt gccacttttg ccgggttgat
     6601 tttccactac atcggtattg gcccgaataa ggaagtggac gatgacgagg aggatcatca
     6661 tgagtaagtc gaaaatgatt gtgcgcacca aatttattga tcgcgcctgt cactggaccg
     6721 tggtgatttg cttcttcctg gtggcgctgt ccgggatttc gttcttcttc ccgacgctgc
     6781 aatggctgac gcaaaccttc ggtacgccgc agatgggacg cattttgcac ccgttcttcg
     6841 gcattgcgat tttcgtcgcg ctgatgttta tgtttgtgcg ttttgtgcat cacaacatcc
     6901 cggataagaa agatattccg tggctattga acattgtcga agtattaaaa ggcaatgagc
     6961 acaaagtggc ggatgttggt aagtacaacg ccgggcaaaa gatgatgttc tggtcgatca
     7021 tgagcatgat tttcgtgctg ttggtgaccg gggttattat ctggcgtccg tactttgcgc
     7081 agtacttccc gatgcaggtt gttcgctaca gcctgctgat ccacgcggct gccggtatca
     7141 tcctgatcca cgccatcctg atccatatgt atatggcatt ctgggtgaaa ggatcgatta
     7201 aagggatgat cgaagggaag gtaagccgtc gctgggcgaa gaaacaccat ccgcgctggt
     7261 atcgtgaaat cgagaaggca gaagcgaaaa aagagagtga agaagggata taatctcttt
     7321 tgaactttaa gctgaaaatg gcgctttaag aagcgccatt tttttattgc gtaaccagac
     7381 ggcgcagtcg cgacacatcc accgtttttt cagcctctgc cagactccag gcgttttgca
     7441 gattcagcca catttgcggc gaactgccga tcaccacgga aagtttaatt gccatttctg
     7501 gcgtcaaagc tgcttttccg gtcagcaatc ggcttgccgt tgagggcgca atttccattg
     7561 ctctggcaaa ctcgcgcagg ctgacattaa gttcgtccag tgattcctga ataatgtccc
     7621 ccgggcgggg atgattggcc attttcatta gtgtctttac tctcagtagc gttccttctc
     7681 tgggaacact atcattattg ttccttccta cgcaacaata atccatttag ccatttcatg
     7741 ttggagagtg tgcgatgctg cgacccgaac atgggagtcg cagcaaagac ctcttagtga
     7801 cggaaatcaa tcaccatacg gccacggatt ttgccttctt ccatctcggt aaagatggtg
     7861 ttgatgtccg ctaacggacg cagggcgact ttcggcacca ctttgccttc ggcggcaaac
     7921 tggaaggctt cggttagatc ctggcgcgtg ccgaccagcg aaccgaccac ttcaatgcca
     7981 tccagcacaa gacgtgggat atccaggctc atagactccg gcggcagacc gacagccaca
     8041 acacgaccgc ctgcacggac agcatcaact gccgagttaa acgcagcttt agctactgct
     8101 gttaccaccg cagcgtgagc gccaccggtt ttctcctgca caatttaggc ggcgtcttcg
     8161 gtgcgtgagt taatcgctaa atctgcaccc atttcggttg ccagttttaa ctgctcatca
     8221 ttgacatcaa tggcgatcac tttggcgtta aagacattct tcgcgtattg cagggcgagg
     8281 ttacccagac cgccaagacc gtagatagca atccactgac cgggacgaat ttttgacagt
     8341 ttaacggctt tgtaggtggt gacccccgca caggtaatgc tgctggccgc cgccgagtcc
     8401 agaccatctg gcacttttac cgcgtaatcg gcgaccacga tgcactcttc cgccatcccg
     8461 ccatcaacgc tgtatccggc atttttaact gaacggcaga gcgtttcgtt accactgtta
     8521 cagtattcgc aatgaccgca tccttcgtaa aaccacgcca cgctggcacg atcgcctggt
     8581 tttaatgagg tgacacctgg gccaacttct gccaccacac cgatgccttc atggcccaga
     8641 attaccccag ttttgtcacc aaaatcgcca ttcttaacat gaagatcggt atgacataca
     8701 ccacaacact ccattttcag cagggcttcg ccatgtttca gtgagcgcag tgttttatcc
     8761 gtaacgtcaa catgatgatc cttcgtaaca actgcagcct tcatagttcc tccttttcga
     8821 atgatgttct gcatagcagg tgaggcaaat gagatttatt cgccactacc cagtatggat
     8881 gagatctgaa aaagggagag ggaaatagcc cggtagcctt cactaccggg cgcaggctta
     8941 gatggaggta cggcggtagt cgcggtattc ggcttgccag aaattatcgt caatggcttg
     9001 ttgcaaagct tcagcagacg ttttcaccgc cacgccttgc tgctgcgcca ttttgccaac
     9061 cgcaaacgca attgcgcggg agactttctg aatatctttc agttccggta gtaccagacc
     9121 ttcgtcgttc agcaccagcg gcgaatactg agcaagcgtt tcacttgctg acatcagcat
     9181 ctcatcggtg atacgtgacg cgccggaagc aataacaccc agcccgatgc cagggaaaat
     9241 aaaggcgttg ttacactggg cgatagggta gattttatct ttccatacca ctggattaaa
     9301 cgggctgcca gtggcgacca gcgcgttacc ttcggtccag gcgataatgt cctgcggtgt
     9361 ggcttccacg cgtgacgtcg ggttagacag cggcatcacg atcggacgcg gacagtgttt
     9421 atgcatctca cggatgatct cttccgtaaa cagcccggtc tgtcctgaga cgccaatcag
     9481 aatatctggt tttacattgc gcaccacatc cagcagtgac agcacatcgc tgtcggtatc
     9541 ccagtcactg aggttttcgc gtttctgcac cagtttggtc tggaaaggca gcaggttcgg
     9601 catcttgtcc gtcagcaggc caaagcgatc gaccataaag actttctgcc gcgccgcttc
     9661 ctcgcttaat ccttcgcgct gggtctgggc gatgatcatt tcggcaatgc cgcatcccgc
     9721 tgaacctgca ccaaggaaga cgattttttt ctcgcttaac tgaccacctg ccgctcggct
     9781 tgctgcgatc agtgtgccga ctgttaccgc cgcagtgccc tgaatgtcat cgttaaaaga
     9841 acaaatttca ttgcgatagc ggttaagtaa cggcatcgca tttttttgag caaagtcttc
     9901 aaactgcaac agcacgtctg gccagcgttg tttcacagcc tggataaatt catcaacgaa
     9961 ttcatagtat tcgtcgtcaa tgatacgcgg attacgccag cccatataca gcggatcgtt
    10021 aagcagctgt tggttgttcg ttccgacatc cagcaccacc ggaagggtat acgccgggct
    10081 gatgccgcca caggcggtat agagcgacag tttaccgatc ggaatgccca tcccgccgat
    10141 gccctggtca ccaagcccca gaatacgttc accgtcagtc accacaatca ctttgatatt
    10201 atggttcggt acgttttgca gaatatcgtc catattgtgg cggttctggt aagagataaa
    10261 cacgccgcgt gaacggcggt agatctcaga aaaacgctca caggctgcgc cgacggttgg
    10321 ggtataaata acaggcatca tctcatcaag atgattgttt accagacggt agaagagggt
    10381 ttcgttggtg tcctggatgt tacgcaggta gatgtgtttg tcgatttcgg ttttgaatcc
    10441 ctgatactgg atccatgctc gttccgcttg ttcttcgatg gtttcgacca cttccggcag
    10501 taaccccagc aggttgaagt tacggcgttc ttccatgctg aaggcactgc ctttattcaa
    10561 caacggaaat tccagcagta cagggccagc gtaagggata taaagcgaac gctgtttttt
    10621 tgtttttggt tccatgtcac tcactctttt ttgaatatcc atccctggca cgcttttatc
    10681 gtctttgctt taccgccagg gcgtcggccc caagtataaa gcagataaaa acaaaaacac
    10741 cattgcgcag gcaatggtgt ttaatcgtca ttgaggactg atggttatga attacttttc
    10801 agcggggcgt tttctgccgg ttgggttatt taccacgctg gatttgtcac cttcggtaac
    10861 tattttgcgc tggttagaaa ttttatggtc cagtccaaga atatgacgtg cctgacggtt
    10921 cgatttcatt ttaactcctc aatcctgttg ctagttttaa ggacaacatc gccgtagcga
    10981 agaaacacgt gctaaacccc taaattaggt tgccgatcaa gcatagcacc ttaaagcgta
    11041 gggtgctggc cactgaccac ataattgatc gtttgctggt agatatcact gaggatgtcg
    11101 ttatcagaag cttccaccca tttggtcagc tccatgagaa tgtcatcttc agtgacaaca
    11161 ccgtgctctg cccgaaggcc ttgctcaatg gcattaacca gagcgggttc tgctgttgaa
    11221 ttttctgcct gataataagt aaacatagtg attctccgtg tctgtgtatt tatggtgtct
    11281 gctacggatc gcagatttat aaagcacatt cagcatggca aatatttgcc gcttcgttgt
    11341 taagattagt cctggttgat gatttttata ttttaacctc atgatattga taggtattat
    11401 tattgtggtg agttgattaa tattaatgca atatctcttg ggttatatat tcccggttat
    11461 ctattgtgga aatttaattt aagtgcagaa gtaatatttc tgccggattt ttattcggaa
    11521 tatcctgctt atcctcgtgc tgtttctcac gtagtctata atttcctttt taagcccaca
    11581 tgagagcaac aatgacaatc cataagaaag gtcaggcaca ctgggaaggc gatatcaaac
    11641 gcgggaaggg aacagtatcc accgagagtg gcgtgctgaa ccaacagccg tatggattta
    11701 acacgcgttt tgaaggcgaa aaaggaacta accctgaaga actgattggc gcagcgcatg
    11761 ccgcatgttt ctcaatggcg ctttcattca tgctggggga agcgggattt acgccaacat
    11821 cgattgatac catcgccgat gtgtcgctgg ataaagtgga tgccggtttt gcgattacga
    11881 aaatcgcact gaagagtgaa gttgcggtgc cgggtattga tgcctctacc tttgacggca
    11941 tcatccagaa agcaaaagca ggatgcccgg tctctcaggt actgaaagcg gaaattacgc
    12001 tggattacca gttgaaatcg taaagcattg ccggatgacg tgtcaggcgc gtgaatgcct
    12061 gatgcgttgt tagcatctct acagcgcccg ccagcaccgg agctcacgtc cgtcctccct
    12121 tattgctaat gattgccgga cttcacatcc gctggttgca agggggcaac gttcgtagaa
    12181 aaaacagcct tgcggcaacg tgcggtttcc cggcagatcc gttttacgta atgcccattc
    12241 ttcctccagc ggtttatcaa tggcggggag ggaatccagc aataatcggg tgtatggatg
    12301 tgccggagcc gttagcacct gttgcgcgtc gcccagttct acaatctgcc cgagatacat
    12361 caccgccacc cgatcgctca tatgacgtat caccgagaca ttgtgtgaaa tcagcacata
    12421 ggtcagcccg tgattttcct gtagcgttac cagtaaattg aggatctgcg cctgcacgga
    12481 gatatccagc gcagaggttg gctcatcaag cacaatcacg tcaggctgcg aagagagtgc
    12541 tctggcaatg gcgatacgtt gccgctgccc gccggagaac gcatgaggca ggcggtcgag
    12601 atactccgga cgaataccca tctgcacagc cagctcctct gccaacgctc gcattacccg
    12661 ctggcgcata ccacctgaaa gctcaaacgg atagcgcgac ataacttcca cggcatccgg
    12721 gatttgcatc tcttccagca ggacaatcgc tttagctctg gcttcccgac gacttattgg
    12781 ttgatgatgg cggatcacgt ccatcatctg aagacctatt cgacgtgtcg gattgagggc
    12841 ggtcatcggt tcctgaaaga tcatcgccac tcgtgcgccg cgccactgac gaagctgttt
    12901 ttcccgagcg ttaagaacat cgtctcccag cagtgaaatc tgcccccgat gtacgcaata
    12961 actgcccgtc ggtagcagac gcataatcag cattgcggtg actgatttac ctgagccgga
    13021 ttctcccacc agaccgacaa tttcaccgcg gttaatctgc aaggacacat tgttgagcgc
    13081 gtgaacatca ccgttaaaac cggggaaact caaatgcagc tgttgaatgt ccagaacggg
    13141 ctgggtcatg actgctttcc tcctgctttc gggtcaagca gatcgcgaat accatcacca
    13201 aagagattaa acccaacggc ggtaagcaaa atagctgccc ccggaaatgc gcaataccac
    13261 cactgatcga gtacatagtt gcgaccattc gctaccatcg cgccccattc agcactcggt
    13321 tgttgagcac ccaggccaat aaatcccaac gtggcggcca ttaaaatcgc gctaccgata
    13381 tccagcgatg cctgcacgat cagcggcggc agggagttac gtaaaatatg ccagctgatc
    13441 agatgccaac gcgacgcacc aaaggttttc gccgcctgaa cataggtata ctggcgtaca
    13501 actaatgctt gcccccgcgc caggcgcaca taaaagggaa ttcgcacaat agcaatcgcc
    13561 agcatggcgt taaataaact cggcccgaga gcggcagcca gtgccattgt cagtaccaac
    13621 gaaggaatcg acagcataat gtccatgatg cgcatgatga tggcgtctgc gcgtccgcca
    13681 agcacaccgg atagacatcc gagtagcgaa ccaatcatac ccgcaatggc aaccacgact
    13741 aatccggcga gaattgattg ctgactgccg accagtacgc ggctgaacag atcgcgtccc
    13801 acttcatcgg tgccaaacca gtgcaccgca gaaggcggca aaaggcgggc ggttaaatca
    13861 atggcgttgg gatcatgcgt cgcaagccac ggtgaaaaaa acatcatcaa tagcattaat
    13921 gcaatgatga ctgcgccggt cacggtgagc ggactgcctt tcagcatcca taccagtttt
    13981 gcaccgttaa atcgcgtttg tttttgtggg cgtacggcgg acgtttcctc gcttagcatc
    14041 attcaccacc tccacgtccg atacgcggat caatccacaa atagagcaaa tccaccacca
    14101 ggttgaccag cacataagca aatgaaacca cgacggcaaa gcccatcact gccgggaagt
    14161 cgagcgcctg tattgatgtt actacccatg cacccattcc gggccaggca aaaacggttt
    14221 cggtgagcac tgcgccatac aacaaatcgc ccagcgccag acccgatagc atcgctggcg
    14281 cggtcacccg ccagtaaccg cgccggatcg ccagggatca gatgcgagat aataaacgta
    14341 atcacgcaga cgcccgccac cacgagcacc agcccccagc agcgttggcg taaaatactc
    14401 cagaaggtca cgttatttac tcatggtatt gatattgaag acctgttcca gcatgggatt
    14461 gaacacaaag cctttcacct ctttgttcat cgccagttgg tagtttttct ggaacaggta
    14521 cacataagca gcgtcatcaa tgacgatttt ctgtgcctgc tggtagtccc gggtacgctg
    14581 cgtctggtcg gtggtcgcaa gcgcattgcg cagtaactta tcgacctcac tgttttcata
    14641 gaacgagcgg ttacccggca gacctttttt gtctgactca aaccagtaat tcataaacat
    14701 atacgggtcg gcaaaatccg gactccagtt gccaatcgca atgtcgtaat cacctttacc
    14761 cactctgtcg cgcatggtag cgttcgccag cttttccagc ttcacattga tgcccagctt
    14821 gttgagactg gattgtgtcg ccagagcaat aggctcccag ttcggatcct tatcggagta
    14881 gagaaacgtc aggctggtgg gtttgctcgt cactttatcc cattcggctt tggctttcgt
    14941 ttcgtcatgg ttgtattgca ttgccgtcgc atcgtagccc cacatgcctt ccggaatcgg
    15001 gccgcgcatc tgttttccgt taccactcag aatgccgtta accatgccct gataatcggt
    15061 agaccaggaa atggcccgac gcagatccgc ctgattaaga ggcgctttgc tgttattcag
    15121 atacaggtag gtgacgcgca gtgacggata ctctgccaca ttgactttgt tttcctgctt
    15181 cagggcgttg agttgatcca ccggcagcgc atcggcaatg tcaatatcac cacgggagag
    15241 ctgcaggcga cgggaggcac tttcaccaat aatttttacc gatactcgct taaagttcgg
    15301 tttattgcca gggtaatgcg gatttggcac cagaactaat tgctgacctt tttgccagct
    15361 tttcagcata aatggtccgg aaccggcggt attttgcgcg aggaagccgc gggcatcatc
    15421 cgctgcatgt tcctttaaca ccgccggatt gataatgtat gcaccgtcat tcgccagcgt
    15481 gtagaggaac ggtgcgaatg gctggctaag ggtaaacttc accgtatgtt cgtcgggagc
    15541 atcaatcttt aaatctttgg gaaatgcttc tgctggcccc tggccgattt ttagtatccg
    15601 ctcaaaagag agttttactg cttcggcagt gaccggtgtg ccatcggcaa atttagcatt
    15661 gtccttcagg gtgaacgacc actctttttg atcgtcagac gctttccagc tacttgccag
    15721 atcgccttca acgtcggttg agcctttatc accgtccgtt ttgtactgaa ccagccgctg
    15781 ataagacggg taggtcaccg tccagtcgtt attatctatt gttaccgccg gggcgagggt
    15841 ttgtggatcg gcggccttac caatcaccag catatctttc ggtacggcgg cgtgcgcaac
    15901 cgagaaagtt gtggcaagga cgagcgcgag caatgtagga cgaaacggta tcgatctctt
    15961 catgacagtt tcctgattaa ctgacgtgct gtgtgccagg ggatataaaa caggtgaatt
    16021 gatcagcgag cagtgggtaa ctcgctgcct agggtaattc gaagtgccac cattcgctgg
    16081 agataccgac aaaaccgcca ccagtcataa tcgcattcag caacagccga ttgcgctgag
    16141 cggcgggcgg gacggaaggg tgataggcat gggagcgctc gtgcatttca tcgaaccctg
    16201 cgcccatatc gaggatgttc ccgtgcttat cacgaagcgt caggtcgatc gccgtgccac
    16261 ggctgtgatt agaaccgacc gtcacatcaa caacatattg cgggtctggg caggcttgcc
    16321 acaacatcgc ctgtgcttgt tgtgggcgat acgcatcgta aaccaccagt tgtaagcctg
    16381 acagctgggc gatactgata cttttcgcca gcgcggtaat cgcatccttg tgtagcagac
    16441 aacgcgcttg ctgataaata gctttacctg tgatgttatc agcgcaggcg tatttcaatt
    16501 cgatctccag atcagggaag attacggcta aatcaaccag ttcggtggta tccgacataa
    16561 acggttcctg tataagacaa aaattgctgc gctttccgct tatgcagatc tcatgccatg
    16621 ccgggataag cgccagaatg ctggcttaaa gttatatttt ttaacttttg atcaacattt
    16681 gtgcagcgta gtgcagtttt ggtgcaagag gggaagttaa ggaaggaatc tcccggaatc
    16741 gtagctgaaa tcacagtatt taagtgacgg tgtcacgtta aatgaaaacc cgcgagtgcg
    16801 ggcgagagga atttgtcaga ttttcagcgg taacacgctg ctcatccagc ctggaatttc
    16861 ttcggcgggt agggggcggg aaaagaaatg ataagcccac gcccatatca cgcaggatct
    16921 gaatgcgctt aaagatttcg gtatcgtgtt ccatcatcat gctttccgtg atttctaccg
    16981 tcagctggtg gccgtcaata ccccaggcgt gcattgcatc agacacctga ttaggcagtt
    17041 gattactgcg aaagtgcagt gccgacaagt taacggaaag cgccgggata tgaatattct
    17101 ggctacgcca ttctgctaac tgacggcaag cttccgcgat gacccagcgc ccaatatttt
    17161 cgatttcacc aatctcttct gcgagaggaa taaaccgtga agggggcaca tgaccatgct
    17221 ggggatcgtg ccagcgcgca agggcttcga tgccgtacag ttcacccgtt tctgcgaaga
    17281 tttgcggctg gtaaacaagt ttcagttggt tattgctaat cgcttctttc agcgctgcgc
    17341 ctaaaaccaa acgctctttt accatttcgt tcatcgccgg gctgaagaac tgccagccgt
    17401 taccgccatt cttgcgaata taatctattg cattgtgagc agtggagagt aggtaatcgc
    17461 ggtttttacc cacgtcgtag ctgatgccaa tactcaaggt aagcgggaat ggtttatcgt
    17521 caatcattat cggcttgctg accacattcc gtagctcatc ggcgatctgg gtaatgttac
    17581 tgacgtcgtt ttccaggctc acgaggacaa actgcgtacc ttcgatacga cagagatact
    17641 gatccggttt gagttttcca cgaaagcgat tgaccacttc cagcaatgcc tgatcggccc
    17701 acgcatagcc aaggctatca atcacatcct gaatatggtc aacaccgatg agatacacca
    17761 ccggagagac ggctttgtcg accaggtcat cgaggtaatt gtgcaggtta ttgcgatttg
    17821 gcagaccggt catcggatca aattggatga gttgttcaat atgctggcgg cttttttcct
    17881 gttccagcgc cagcgcggcc atatgctgac tgatatctgc cacgcgttcg ataaaggcgc
    17941 tggtttctgc tcctgacgag gttttaattt gcaggatccc cgcaggcgcg ccatcacgct
    18001 gacgaatggt cgctgaccag ctttgcgcat tttgaacttc tgcaccgtgc gaagatgacg
    18061 cccagtgtat cggcatcccg ttgcgcagtg cgaacagcga aacatgcgat tcgttgagaa
    18121 cagattcgat gttacgacaa atgatttccc ccatttcatg aaatggcggg ctgctgcaca
    18181 tggcggcgag aatattgcct tcaagctggc ggatttgccg ttcttcggtg atatccgaga
    18241 aagtcattac caggttctgc agatgcgcga gcacgtcata aaccgggctg atagaggctt
    18301 taatccagat tttttcaccg gtgcgcgtca acagcagaaa ttcgtcctga tcgcgggcgg
    18361 ttttccatag caactgttgt aaacgaatgc ggttatcggc agggaattca ggaatgttca
    18421 ggagtgtatc gggctgcata ccgctggctt cgctaatgca gtaaccaaac atgcagtaac
    18481 caaacatttc ggtaaatgcg cgattgcact gcacaatatg gcgttccgga tcgaggacaa
    18541 tcaccggtcg gtcgagatgg tcaacggcaa taatcaattg tcgggtctgt tctttttgcg
    18601 ccatttctac gctggcatcc cgtaccagcg ccaggtaata aactttcccc tcggcgctca
    18661 ctttcgatag cgcaaaacgg gtccagattt tactgccgtc ttttttctcc agctgcagct
    18721 cccgactcat cccctcgacg cgcgctttac caccttcacg gttatgacga atgtattcag
    18781 gatgcgcagg acgcaaatcc cgcggaatca gcatatcaat gttattgcca atgacttctt
    18841 cacgtttgta cccccagagc ttctctgcgg cggggttgaa aaacatcact tcatcatttt
    18901 cgttaattaa caccgcgccc atcatatttt gttcaagggc ggggaaaaaa atgccatcgg
    18961 cggcattttc cgcatcggtt agcttcatga ttacctctgc atcctggcgc atctaaagac
    19021 tggctttcca gagttcaaca cggtttctac ctcgtctttt ggcgatatac agagcttcat
    19081 cggctatttg aatgaggcgc tcatagtcag gatgaccatt aaacatggcg gcaccgatgg
    19141 aaagtgagag ggcaatatcc tcgccgtttg cggctttcag tttggttttc tccacccgac
    19201 tgcgaatacg ttctgcggta cgtaacgttt cgttttcaga agcttcagtc aaaacaatga
    19261 taaattcatc gcccccgtag cgaaaaacat aatcactact gcggacgttg tcataaaagg
    19321 cctgagaggc tttacgcaga atttcatcgc cagtgttatg gccccacgta tcgttgatct
    19381 ctttgaattt atcaacgtca ataatcagca ctgacagcgg tgtaccggtc cggttggcat
    19441 gggcaatttc gtgtttgaag atagtcggca ggaagcggcg gttaagtaat ttcgtcagta
    19501 catccatacc gacttcgtgg cgcgatactt cttcaaacaa ttcacgcagc aaggtaataa
    19561 tttgcgatac agtatttctt atctgtaata aaaatttcac ccgcagactt ctgttattca
    19621 aattcctggt gttacgcatg gtttgattga atataccgtc gaaatcctga atcagacggg
    19681 agatatgcct acttcggcaa tgccactaaa ataatgtcga cctttatggt taaaccacag
    19741 gccaaaatca gcctggctta aaggcaaact actgcctaaa tcagaatcca gcaggatttt
    19801 atagataata tctatttccc atgaaagtat tgaggctatt tgccgttctt tttcttcttc
    19861 ggcgttttct agtaacgaga agatacgata gttttcatct tcctttgagg cactactgtc
    19921 actaaaggta aacgcgcggg tcatcacttc catagcgata tcaatactgt taatcgagaa
    19981 atggtagacc tgaagttttt ctgcggcaga ataatccgaa gagaagatta ccggatagag
    20041 gatttttttc agcacccgaa accccatctc gacaatttct accggaattc ctatgcgggc
    20101 atgcacttcc gcgacggtat gctggatttg tattagcctt tcgacatcgt caacttgggc
    20161 agaaagcacg ttaataatcc agcgttccat cgtacttttc aactgccgct caacttgttc
    20221 attactcaag aattcttcgg catgcgggtc gatgcggaca attcgataaa actcgatact
    20281 cagataatga gcatgcgcaa cggcaatttc cgcggcttta gcacgaatga gtggatctgc
    20341 ctgttcgaca agtccggtcc actcatcttt cattctttta aaatacatct ccataattca
    20401 cacccttata aggctgggaa atcagacgga atcaaaacgc aacgtgcgag atcgactaac
    20461 tgcaccatat tctcctgaaa tatgaagata tactgaaaag aaataagcga cttaggacag
    20521 tttcaatcta cgctactgtt cttcagaaga gtatagccca tcgtaattat ttttcggtga
    20581 cagcgaatat cgtatggttt ttcatattca tacattttta ttagggattt atagctgttt
    20641 aactaagtgt ggttaattgc gtggttaatt tgacttatgt aagcatgatt actagtggga
    20701 tatttcacaa ggtgtaacaa gccggtgggt aaagcaccgg cttgttacaa agtaagcatg
    20761 ggagtttaac tgccccaacg actttgcaga tagctgaccg cttgttgagt ctgcggttta
    20821 ttcagatagt cctcacggaa caagatggtg ccgctaattt ctggtaccgc atcgttaaga
    20881 tcgagctgct ttttcagttc cggtacgccg ccgttaatca tccagtctgg ctctatcttt
    20941 gaaggttcac ccactttata gaaggcgata ccgatataca ggcgggtcct ggtcggttta
    21001 acgacatccg cccaccattt tgccaacacg tcataacgcg cggcactccg tgagaacggc
    21061 cagtaaattt ggggagcaat gtaatccagc aatccttgtt ccacccatcg acgggtgtca
    21121 gcgtaggatt cgtcataggc tgccgcgcct cgggtatcgg aaccgagcgg atcgtgtgat
    21181 cggttacgcc acacgcctgc cgggctaacg ccaaattcga ctcccggctt aatgctttta
    21241 atggtgtgcg atacttttgt aattaactgc tgagtattgt tgcgccgcca gtctgctttt
    21301 gacgcaaatg cgcctccgta tttacggtac gtttcgttat catttagccg tgaacccggt
    21361 gactccgtat agaaatagtc gtcaaactgc acgccatcta ccggatagcg ggaaaccact
    21421 tcggcgacta ttgatgtgat ccagtcctga acctcaggga tgcccgggtc gaggacaaag
    21481 cgatcgccag acgttctgat ccagtcacgg tgttgcacat agacgctcgc cggttgttga
    21541 gacagagtgc tattcagttc cctgatagta ccgggcttcg tattaaccga tacgcgatag
    21601 gggttaaacc aggcgtgtac tttcatccca cgcttgtggg cttcgtcgag cataaattgc
    21661 agcggatcgt aacccggatt ttcaccaatc ttaccggtca taagatcgga ccacggcaaa
    21721 attttcgatg gccacagggc ggttccgtcc ggcttgactt ggaaaaagac cgtgtttata
    21781 ccgagacgtt gcagatgatc cagtttgtcg atcatcgcct gttgttgtac acgggcccgg
    21841 ctggtggggt tactaatgtt aaccgaggaa accggtggcc agtcgaggcg agacaccgtg
    21901 gccagccaga tgccacgcat cggttgtgac gattgttgcg tcgtggctgg tggctttgaa
    21961 cctgctggtg gtgtcaccat ggactctgga ggcgtgcttt tacaactaca cagtaaaagt
    22021 gccagtgcaa ccagtatcgc tggtcttcta atcgttaatt tcttgtttcg ggagcagata
    22081 tccatatgtg ctggtttccg gtaaacagat gtgcgctcat tctcgtactt atcccggtta
    22141 agtcaatacg acagcaagca cgaaaaaggg agcgatgaat tatcgctccc ttgtcttata
    22201 accattcaga catggttagt gtttcttgtc attcatcaca atatagtgtg gtgaacgtgc
    22261 acgcgggtgc aggaagaagt gaccttttgg tgcgttctga ctgttgattg gctccagagt
    22321 gacgccggta ttcgctttgc ctttacgatc atgaacagca tagagaataa agggcagggc
    22381 aagtaccacc aggaaactaa cgaccagtaa ttcaacatac atatcggtag agtcaccctg
    22441 gatgttatcc ggcggcagga aggaaacaat aaacgccata attgaagtca gcagaccgac
    22501 aattgccacg accagtttca cccctttacc accagggata ttaaatgtgc gttttaagtc
    22561 aggatgttta agaaccaaca caatgtagcc aataaacagc atgaaataag cacacagata
    22621 aatcaccacc gtcagcgcca gtgcgatcag gaaggacatg ttgttaccgc caccggtatt
    22681 ggtgaggatg atcaacgcga tagaagtaat caccagctgc gaaatgacca gcgttaccgg
    22741 tacgccattt ttgttcattt tagcgaatgc cgctggcagc aggtttttct gcgctgtgac
    22801 atacatcccg cgagaaggac caacaatcca ggaggcgatt tccgccagaa cacccagcaa
    22861 cagcagtgcg gagatcacgc gaaccgtcca ctcaatttcc ggtgccacat gggacatcag
    22921 aacggtaaag gtttgcatta cccctgcgga gaggttgatt tcattacccg gaatgaccat
    22981 cgcaatagat aaaccgccaa cagagcttaa gcagattgcc gccaccatca gcagtaacat
    23041 agccagtgga tagtcgcgcc cagggttgct catttcattg acgtgagttg cggaagcttc
    23101 tacgcccata taactcaaaa tgaaggcaac aaatacaacc agtgtgccca ctttagagaa
    23161 gtcagggaag aaggtcttcg aatccatttc gatagcaacg ggggcaccgg agtgcagata
    23221 aatagccgct aatgcgatca aaataaatgc aggtaacagg ataccggcga agaagccaac
    23281 tttagcaatt cgcgccgtgt atttcgtgcc accaaactgc gttaatgcca gcgcccaaag
    23341 aatgatgagt gctgcaatag ttttggtaat ggggtcttca ttcagcgctg gccatttcag
    23401 gatgtaggag agtgccccta acacgaaata gagcatcgga ataaaaccaa tggcgatttg
    23461 cagatagcca aatgagatcg ctgcaaatcc ccatctcggc cccagagtat ttgataccca
    23521 ggcgaagaca ccaccttctt cccagccgtc gacggtggcc atttccgcag cacaaagtcc
    23581 cacgggaata aaccataaaa tcccgcctaa tagcaggaag aagactaatg aaaagcccga
    23641 tgttgcgaag gtagggtatt cataaacagc cattaccatc gatgccgtta tggcaaagaa
    23701 tccgagtaat gtgagctgct tagctctacc tgtctgtact gatgtagcca tattatcccc
    23761 ctaaaacggt attcctgtcg gaaccgcacc tgttttgttc tgaagcgtat tcagaacaat
    23821 attttccgtt gctaatgcca gtgaacagac tttggaaatt gtcccgaaac gggttcgttt
    23881 cgggacaccg ttaccgttaa acgttatcag gtatgtttaa agctgttctg ctgggcaata
    23941 ccctgcagtt tcgggtgatc gctgagatat ttcagggagg ctttgtagtc ttccagcaac
    24001 agttca
//
LOCUS       DAJTNA010000049        23954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTNA010000049 DAJTNA010000000
VERSION     DAJTNA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23954
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.050"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            110..716
                     /locus_tag="OO161_001626"
                     /pseudo
     CDS             110..716
                     /locus_tag="OO161_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            819..1322
                     /locus_tag="OO161_001627"
     CDS             819..1322
                     /locus_tag="OO161_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCR5462742.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTPAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSKLRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1389..2846)
                     /gene="nuoN"
                     /locus_tag="OO161_001628"
     CDS             complement(1389..2846)
                     /gene="nuoN"
                     /locus_tag="OO161_001628"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5462743.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2853..4382)
                     /gene="nuoM"
                     /locus_tag="OO161_001629"
     CDS             complement(2853..4382)
                     /gene="nuoM"
                     /locus_tag="OO161_001629"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5462744.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4546..6387)
                     /gene="nuoL"
                     /locus_tag="OO161_001630"
     CDS             complement(4546..6387)
                     /gene="nuoL"
                     /locus_tag="OO161_001630"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5462745.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6384..6686)
                     /gene="nuoK"
                     /locus_tag="OO161_001631"
     CDS             complement(6384..6686)
                     /gene="nuoK"
                     /locus_tag="OO161_001631"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5462746.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6683..7237)
                     /gene="nuoJ"
                     /locus_tag="OO161_001632"
     CDS             complement(6683..7237)
                     /gene="nuoJ"
                     /locus_tag="OO161_001632"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5462747.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7249..7791)
                     /gene="nuoI"
                     /locus_tag="OO161_001633"
     CDS             complement(7249..7791)
                     /gene="nuoI"
                     /locus_tag="OO161_001633"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5462748.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7806..8783)
                     /gene="nuoH"
                     /locus_tag="OO161_001634"
     CDS             complement(7806..8783)
                     /gene="nuoH"
                     /locus_tag="OO161_001634"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5462749.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8780..11506)
                     /gene="nuoG"
                     /locus_tag="OO161_001635"
     CDS             complement(8780..11506)
                     /gene="nuoG"
                     /locus_tag="OO161_001635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5462750.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11559..12896)
                     /gene="nuoF"
                     /locus_tag="OO161_001636"
     CDS             complement(11559..12896)
                     /gene="nuoF"
                     /locus_tag="OO161_001636"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5462751.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12893..13393)
                     /gene="nuoE"
                     /locus_tag="OO161_001637"
     CDS             complement(12893..13393)
                     /gene="nuoE"
                     /locus_tag="OO161_001637"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5462752.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13396..15198)
                     /gene="nuoC"
                     /locus_tag="OO161_001638"
     CDS             complement(13396..15198)
                     /gene="nuoC"
                     /locus_tag="OO161_001638"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5462753.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15292..15954)
                     /gene="nuoB"
                     /locus_tag="OO161_001639"
     CDS             complement(15292..15954)
                     /gene="nuoB"
                     /locus_tag="OO161_001639"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5462754.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15970..16413)
                     /gene="nuoA"
                     /locus_tag="OO161_001640"
     CDS             complement(15970..16413)
                     /gene="nuoA"
                     /locus_tag="OO161_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5462755.1"
                     /translation="MSMSTTTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17043..17981)
                     /gene="lrhA"
                     /locus_tag="OO161_001641"
     CDS             complement(17043..17981)
                     /gene="lrhA"
                     /locus_tag="OO161_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5462756.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18901..20118
                     /gene="alaA"
                     /locus_tag="OO161_001642"
     CDS             18901..20118
                     /gene="alaA"
                     /locus_tag="OO161_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5462757.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20202..20801
                     /gene="yfbR"
                     /locus_tag="OO161_001643"
     CDS             20202..20801
                     /gene="yfbR"
                     /locus_tag="OO161_001643"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5462758.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20860..22692)
                     /locus_tag="OO161_001644"
     CDS             complement(20860..22692)
                     /locus_tag="OO161_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5462759.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22779..23429)
                     /gene="hxpA"
                     /locus_tag="OO161_001645"
     CDS             complement(22779..23429)
                     /gene="hxpA"
                     /locus_tag="OO161_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5462760.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(23440..23934)
                     /locus_tag="OO161_001646"
     CDS             complement(23440..23934)
                     /locus_tag="OO161_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5462761.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
BASE COUNT         6132 a         6611 c         5986 g         5225 t
ORIGIN      
        1 tatagatcta acaaccgtaa taaaacaaat actcataact cattataaat aatgataaaa
       61 taacgtcttt tattgataat ttatatatga aaaaaattcc ttttgttata ttgtttctga
      121 tttcactccc tctgattatt tttaccaacc ctgggtcact gcattaccac caacgccccg
      181 acacgtcagt cctgagcaat tagaaaaaac agttcgttat cttacacaaa ctgtgcatcc
      241 acgtaatgcc gacaatattg ataatctgaa taggtccgta gagtatataa aagagttctt
      301 tgtcagtagc ggtgccagag ttaccgcgca gggcgtcccc gattggctat tacgactctg
      361 cgcctgatag ccaggattac ctttacccgc aatgtcctgg ctttatcccg atcgggggga
      421 ctttattgcc tggtcggcag aatacaggat atcaacgccg ttcgtcaggt aaaaacggca
      481 ttgttatcat cccgggattt atctgtttat tctatgaatg ccccatggtt tattcccggt
      541 attgatttct ctgaccacct gaattattgg caacacgata ttcccgccgt aatgattact
      601 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgatacagc agacagattg
      661 aattatcaga aaatggctca gatggtgtta taaccttgtt acacaacagt aaataataaa
      721 aatttctcga ggcgggtcca gaaacagcga catgtacatt ttattttccc tcgtctgttt
      781 taagatgcga aacataaaat tcaatggagg aaaaataaat gggaatgatt ggttattttg
      841 cagaaattga ctcagaaaag attaaccaac tgcttgagtc tactgaaaaa cctttgatgg
      901 ataatataca tgacacgctt tcaggattac gccgactgga tattgataaa cgctgggatt
      961 ttttacactt tgggctgaca ggcacccctg cttttgatcc cgcgaagaat gatccgctga
     1021 gtcgtgccgt gcttggtgaa cacagcctcg aagatggcat agatggcttc ctgggattga
     1081 cctggaatca ggaattagcc gcgacaatcg accgactgga atcactggat cgaagtaagc
     1141 tgcgtaaaca gttttccatt aagcgactca atgaaatgga gatttatccg ggagtgacct
     1201 tttcagaaga gttagagggg caactctttg cctcgatcat gcttgatatg gaaaagctga
     1261 ttagcgcgta tcgacgtatg ctgcgacaag ggaatcatgc gctgacggtt attgttgggt
     1321 gatggtgtca gagcgccaaa aaaaaccgcc gaacatgtcg gcggctttct gacttacaaa
     1381 ctaacagatt acatcagcgg cattgctaac cgcacaatac taatcagcgg ttgtggccat
     1441 acacccagca ccagtaccaa cagtgcagaa atcagcacca cgataccgcc cgcgctgtac
     1501 tgccagtttg acggtgcatc gcgacccggt tgttccggtg cgtgcaggta caggctcacc
     1561 gccacgcgca ggtagtagta gaggccgatt gccgaaccga caaccacggc acccaccagc
     1621 caccacaagt gtgcctggac accgaccgcc agcacgtaga acttaccgat aaagcccagc
     1681 gtcatcggga taccggccag agacagcatc atcaccgtca tcactgccgc gaggatcgga
     1741 cgatgccaga acagaccgcg gtaagagaac agggaatcag catccgggcc acgatacggg
     1801 ctggacatca ggctgaccac gccgaacgcg ccgaggctgc tgaacagata accggccagg
     1861 taaaccccta ccgcttccat cgacatctcg ccggtttgca gcgcaatcag cgccaccagc
     1921 agatagccga ggtgagagat agatgagtaa ccgagcagac gtttgatgtt ggtctggctc
     1981 aacgccatca ggttaccgaa gatgatggag gcaaaggcga taatcgccag tactacgcga
     2041 atcgcttcgc tgtcacccac cggtgcatag aggaacagac gcatcaccac accgaagata
     2101 gcgattttgc tcgccgtcgc caggaaagtg gaaaccggcg caggcgcgcc ctggtatacg
     2161 tctggcgtcc acaggtggaa cggcaccaga gagagtttga agccgaggcc aacaatcatc
     2221 atgccgaaac ctgccagcaa cagcggctcg ttgagcatac cgtcgccgag gtttttaccc
     2281 agcgcgacaa acgacaggtc gccagactgc gcatacacca gcgccatacc gaacagcagg
     2341 aaagaagacg ctgcggcaga aagaatggtg tatttgatac tggcttccag cgaacgtttc
     2401 tggcggaaag cgtaaccgac caggccaaac agcggcaaag agatcagttc aataccgagg
     2461 aacagagacg ccagatggtt tgcattcgcc agcaggatcc cgcccagcgc ggcaattaac
     2521 accaacaggt agaactcatc cttgttgtcg ttatagcctt caagccacgg gtaggcgaaa
     2581 gtacaggtgg cgaggctcgc caataatacc agcccggtgt aaagcatggc gaaaccatca
     2641 acgcgcatca gcggcgtaac gtccatagcg cccgcctggc caacaaacca gagcgaaacc
     2701 agcgccgcgt taagcccaat aaccgagagc gtagcgttga ggaaatgatt gcgtcgccac
     2761 gcaatggaga gcatcacaac caccaccgtt aagccgacga tcagcaacgg tagcagtgcg
     2821 atcaggtttt gtggagttat tgtcatggcg atttacggcc ttgtagtagt aacggaatta
     2881 acaaaccact gctggatatt gccaatcgcg gagtgcgagg tatccagaat cggctgcgga
     2941 tagaagccca gcagtaccag cagcaccacc agcaacagga tcataaacag ctcacgcagc
     3001 gacatccctg gcagttcctg gctggcaatc tggcttttcg ctttaccgaa gtaagcgcga
     3061 tgcaacatcg ccagcgaata aacagatgca aagaccagcc caaaggtaga gataacggta
     3121 atcaccggga caacctggaa gctgccgaac agaatcataa attcgccgac gaagttaccg
     3181 gtgccaggca tcccaagcgt tgccaccgca aagaacagcg acaatgctgg cagccatttc
     3241 atcttgctcc acagaccgcc catcatgcgc atgtctcggg tatggatacg ttcataaagc
     3301 tgaccacaaa gaataaacag acccgccgcc gacaaaccgt gcgcaatcat ctggattacc
     3361 gcgccctggt aggccaactg gctgccggtg tagatagcaa tcagcacgaa gcccatgtgg
     3421 gaaaccgagg tgtaggcgat cagacgtttg atatcggtct gggcgaaggc catccacgca
     3481 ccgtagaaga tgccgataac acccagccac atagcaattg gcgcgaactc tgccgacgcg
     3541 ttcgggaaca gcggcaggga gaaacgcagc aaaccgtaag cggcagtttt cagcaagatc
     3601 cccgcgaggt caacggaacc ggcggtcgga gcctgggagt gcgcatccgg cagccagcca
     3661 tgcagcggaa ccaccggcat tttgactgcg aaggcgatga agaagcccag cattaacagg
     3721 tattccacac cactggacat tggcgtattc agcagctctt catagttgaa ggtccagacg
     3781 ccggtcgcat tgtagtgaac aaaaaccagc gccaggatgg caatcaacat caccaggcca
     3841 ctcgcctggg tgtaaatgaa gaacttggtt gccgccgtga tacgcgtttt accgtcagag
     3901 gctttatgcc cccacagtgc gatcaggaag tacatcggca ccagcatcat ttcccagaag
     3961 aagaagaaca ggaacatgtc gatggcaagg aacacgccga taacgccgcc caggatccac
     4021 atcaggttga ggtggaagaa gccctgatat ttttcgatct ctttccacga acagagtacc
     4081 gccagcacac cgagcagacc ggtcagcacg accatcagca gcgacagacc gtcaatggcg
     4141 agatgaatag agataccaaa acgcgggatc cacggcatgt cgaattcaga ctgccactgc
     4201 ggaattccgg cggattgcgt cagtgaataa ccgccctgca accacagttg cagcgacagc
     4261 gccagcgtca atcccatggt gatcagcgcg atccagcgtg gcaccttgac gtcaaagcgt
     4321 tcggtctgcc agcacaggaa gccgccaata aagggaatta atattagcca gggtagtaac
     4381 atggcgatct ttattccttg taaaagtccc gtcaggaccg gattttcaac gaattctcac
     4441 gacagaaaat tctcttcgga ctgggggtag tgtcggatgc gccgcgaagg cagcttatcc
     4501 gacctacgtt cttatatgcc caggggcaat cccacaatcc ttaactcaac gcagtaccat
     4561 caacagtgcc agcaccacga ccgcaccgat gctcatggat gccacatacc agcgcagata
     4621 accgttctcg cttaacagca ggcctttacc tgcaaagcgg gaaagaaccg ccggaatgtt
     4681 catcattgag ttcagcggat cgcgtttcag caaccaggca atacccagga acggcttgac
     4741 gaacactttg tcgtacagcc agtcaaagcc ccaggcgttg taccaccagg tgcccagcag
     4801 acggcccggc gcactgttgg caatggaggt caccagagta cgtttaccct gccacagcca
     4861 ggctgccagc agaatgccga ccaccgcgac cacgccagag gtaatttcca gggtcaacat
     4921 gctgccgtgc gccagttccg tcgtttgcgg aagcacgctc tgcagcggcg gtacaatcag
     4981 tgcgccaacg aaggtggaaa ggatcagcag cacaatcagc ggcaggctgt gagttacccc
     5041 tttcacggcg tgagcgtgaa tttgttcttt tccgtggaag acgatgaaaa tcatacggaa
     5101 ggtgtagagc gaggtcataa acgcaccgac cagacctgcc accatcagat tgatatgacc
     5161 attcgccatc gcacccgcga ggatctcatc cttactgaag aagcccgcag tgaccagcgg
     5221 tagtgccgac agtgctgcgc cgcccaccag gaagcagaaa taaaccagcg gaatagattt
     5281 acgcagaccg cccatcttga agatgttctg ttcgtgatgg caggccagaa tgacggaacc
     5341 ggatgccagg aacagcagcg ctttaaagaa cgcgtgagtc atcaagtgga aaatcgccgc
     5401 atcccatgcc tgcacgccaa gcgcgaggaa catgtagcca atctggctca tggtagagta
     5461 agcgagaaca cgtttgatgt cggtctgtac cagcgcggca aaaccggcca gcagcagcgt
     5521 aaccgccccg acaatgccca ccagatgcag aatttccggc gtcatcagga acaggccgtg
     5581 ggtacgggcg atcaggtaga cacccgcggt taccatggtt gcggcgtgga tcagtgcgga
     5641 gacaggcgtc gggcccgcca tcgcgtcggc aagccatgtc tgcaacggca actgcgcaga
     5701 tttaccgacc gcaccgccca gcagcatcag cgtcgcccac atcagcatgt tattgccgtc
     5761 agcaaagtgc gctggtgcca gttccaccat ttcgcggaag ttcagggtgc ccagttcgtt
     5821 gtaaagaatg aacagcgcga aagcgaggaa cacgtcaccc acacgggtca cgacgaacgc
     5881 tttcattgcc gctgcgccat tcttcggatc ggtgtaatag aacccgatca gcagatagga
     5941 gcacaggccc acgccttccc agccgaggta catcagcagc aggttatcgg cgagaaccag
     6001 aactaccatg ctggcgatga acaggttggt gtaggcgaag aagcgagagt agccctcttc
     6061 accgcgcatg taccaggagg cgtacatgtg aataaggaaa cccacaccag tgaccaccga
     6121 gagcatggtc agcgacaggc cgtccagcac caggttaaaa ccgatgttaa agtcgcctac
     6181 cgacatccac gtccacagcg gctggctgta tgcctgctcg ccgttagcga agaaatcaac
     6241 gccgataaag gcggttacca gcgccgccag gcccacagag ccaacgccga cgatcgccga
     6301 aacgttttca gaccagcgcc cacgggagaa cgccagcagg acgaagccaa tcaatggcaa
     6361 aataatggtt aaggcaagca tgttcatccg cgcatctcac ttactgaatc gatgttcagg
     6421 ttctggcgac gacggtgaag ttgcagcagc agcgcaaggc cgatactcgc ttctgccgcc
     6481 gcgaggctga tggcgagaat gtacatcacc tgaccgtcgg tctgacccca gtagcttccg
     6541 gcgaccacaa aggccagcgc ggaggcgtta atcatgattt ccagaccaat cagcataaac
     6601 agcagattgc gacggataac cagaccggtt aagccaagaa cgaataagat tgccgagagg
     6661 atcagtccat gttgtaaggg gatcatgcgt gctcctccgt ttttcttttc gcgctgtcgt
     6721 ctttacgatt gctcagcact tcacccgcac gctcttcacg accgacgtgg aaggccacaa
     6781 ccagacccgc gagcagcagc atagaagcca gttccaccgc cagtacgtaa ggcccgaaca
     6841 gcgtaatacc cactgcttta gcactgattg gcgtaccgtc gataccctga tcattaacac
     6901 caaggatggc gtaaacaatc accaccagca tgatggccga caaaattgcc ggaccaatcc
     6961 acacctgcgg tttcagccac tggcgttcct gttcgatttc tgaaccgccc aggttgagca
     7021 tcatcaccac gaacacgaac agcaccataa tggcacccgc gtagacgata atttccagcg
     7081 caccggcgaa gtaagcgccc agtgagaaga acaccccgga aatcgccagc agcgaaataa
     7141 tcaggtacag cagtgcgtgt accggattgg tatgggtgat cactcgcaag gtcgcaagta
     7201 tggctatcag gccacagata taaaaagcga actccattgc ccctctcctt acggtaacag
     7261 gctcttgacg tcgataggct tggcttcgtt ctctgcttcg cccttatctt tgccgtcgat
     7321 tgccatacct gccatccggt agaagttata ttccgggtat ttgcccggac cggagatcag
     7381 cagatcctct ttttcgtaaa ccagatcctg gcgcttgtat tcccccattt cgaaatccgg
     7441 ggttaactga atcgccgtgg tcggacaggc ttcttcgcac agaccacaga aaatgcagcg
     7501 tgagaagttg atgcggaaaa attccgggta ccagcgaccg tctttggttt ctgctttttg
     7561 cagcgagata cagccgaccg ggcaggctac cgcgcagagg ttacaggcta cgcaacgctc
     7621 ttcgccgtcc gggtcgcggg tcagaacgat acgaccacga taacggggcg gcagatagac
     7681 cggctcttcc gggtacattc gcgtttcgcg tttggcgaac gcgtgcaggc cgatcatcca
     7741 gatactacga acctgggtgc cgaaacctac taacaattct tttaaggtca tggtcttatt
     7801 gccccttatt gcgcctgcca gagaatgaca gccgccgtta ccagcaagtt gatcagcgtc
     7861 agcggcaggc agattttcca gccgaaggac attacctggt cataacgcgg acgcggtaac
     7921 gacgcacgaa tcaaaatgaa catcatcata aagaacgcgg ttttcagcgc gaaccagatg
     7981 aatggcggta acaacgggcc ttgccagcca ccgaagaaca gcgtcaccat caatgcagag
     8041 atggtcacaa tcccgatgta ctcacccacg aagaacagac cgaacttcat accggaatat
     8101 tcaatgtggt aaccatccgc cagttcctgc tcggcttccg gctggtcaaa cgggtgacgg
     8161 tgacataccg ccacgcccgc gatggcaaag gtaataaaac caaagaattg cgggataacg
     8221 ttccacacat gcgcctggct gttgacgatg tcggtcatgt tgaatgaacc ggcctgcgcc
     8281 accacgccca tcagggaaag cccgaggaac acttcgtagc tcagggtctg cgcagaagca
     8341 cgcatcgcac ccagcaacga gtatttgttg ttacttgacc agcccgcaaa cagcaccgcg
     8401 taaaccgcca gacctgccat catcaggaag aacaaaatcc cgatgttcag gtcggcaacc
     8461 acccaacccg gactgactgg cacaatcgca aaggccagca gcagcgaggt aaaggcaatc
     8521 atcggtgcca gggtaaagat gacgcgatcc gagaatttcg ggatccagtc ttctttaaag
     8581 aacattttga tcatgtccgc aaccagctgg agcgaaccgc cccagccaac acggttaggt
     8641 ccataacggt tctggaacag acccagcagg cgacgttcgc caaagctcat gaatgcccca
     8701 caggtgacaa ccaccagcag gatcaccacc gctttgagga tggtcagcag gatctcaatc
     8761 agttccggtg atatccaact cattgttgtg cctccttgag atcctcaaga tgcgcgccag
     8821 ccagcaccgg agcaatgccg gacataccca tcggcaagcc cacctgccct gccgtcagtc
     8881 cttcggcgat ttcaaccggc agcgtgaccg tgttgccatc gtaactaaag gagacgcgtg
     8941 tacctgcgtt cacacccaac ttcgcggcat ccgctgggtt gagtttgatg tacggctgcg
     9001 gcatacggct ctggaagacc ggagcacgct gtgacaattc atcgctgcca aacaggtggt
     9061 aatacggcgc aatacgccat ttcccgtcct gcggctggaa gcgtgccggt acgctggtga
     9121 agtaatccag accattctcg ctggtttcaa acagacgcac gcccggatcg ccaaagcgca
     9181 gtttgccgcc cacttcgtcc tggaatttgt tccacgcctg cggggagttc cagcccggtg
     9241 cccaggcaaa cggcacttgc gaacggtgcg cggtcggctg gttgttacct tccatcgaga
     9301 aggtgaacat ggtgtcaata tcctgcggct gacgcggctc atgaacgctg atattggcgc
     9361 gcatggcggt acgaccgctg taacggtgcg gttcacgggc cagtttctgc ccacgaatac
     9421 ggaatgtcgc atccggcgca gcatctttga tacctgccag ttccgggatt ttcgccacaa
     9481 cagcgtcaat cacatggtcg agctgcgtcc agtccacttc acggctcagc agggtgctgt
     9541 gcagcgagtg cagccagcgc cagctttcca gcatgacagt tttgctgtcg taataagccg
     9601 gatcgtaaac ctggaagaaa cgttgggcgc gaccttcatt attaattacc gtaccgtcgc
     9661 tttcagcaaa gctggcggcg gaaagtacca gatgggcgtt ttccatgatc gctgtacgct
     9721 gatggtcaac caccatcacc agcggtgctt tagccagcgc agcattcacg cgggtagcag
     9781 aagcgtgacg atgcagatcg ttttccaaca ccaccaccgc gtcggcgcgt ccggtttcca
     9841 gttcggttaa cgcttcttca agcgaaccgc cacccataat gcccagcccc atgctgttga
     9901 cggaacgggc aatcatggtg ataccgacgt cagcgccgcg acctttcagg gctttcgcga
     9961 cgttagccgc cgcctgaatc acctctaagc taccggcgtt cgtcccggag ataatcaacg
    10021 gtttcttcgc acctgccagt gcctgcacga tgacgtcgat tttgctttgc agctcaggtt
    10081 cgataccgtc aaccgctggt gcagagttat ccagcgcatg ggcgatggca aaacctaaac
    10141 gcgcctgatc ttcaaccggt gcgcggtaag tccacgccgc gatatcatcc agacgggtgt
    10201 catcaacgtt ggtaacaaac agcggatgct tcgcacgttg accgatgtta aggattgccg
    10261 caatctgcca gtcagccact ttctgtgctg ccgccatttc gcgcgcttta cctttcaccg
    10321 cctgacgcac tgccagcgcg acgcgcgcgc cggtctgggt aacgtcttcg cccagcacca
    10381 gtaccgcatc gtaagattcg atttcgcgca gagccggagt ataaatgccg ccttcacgca
    10441 gcactttcag cgccagttgc agacgttcct gctcaccgtg agcgataccg gtgtagaagt
    10501 tttcttcgcc caccagttca cgcagtgcaa agttgctttc cacgctggca cgcggagaac
    10561 caataccgat cactttcttc gactgacgca gaatatctgc cgcgccctgc attgcctgtt
    10621 cggcgttgag ggtaatgaaa tcatcgccac gacgctgtac tggctgacgc ggacgatcct
    10681 tcaggttgac gtaaccgtaa ccgaaacgac cacggtcgca gaggaagtag tggtttaccg
    10741 taccgttgta acggttttcg atacgacgca gttcgccgta acgttcaccg gggctgatgt
    10801 tacagccgat ggaacattgc tggcagatgc tcggcgcaaa ctgcatatcc catttacggt
    10861 tgtaacgctc ggagtgcgtt ttgtcggtaa atacgccggt cgggcaaatt tcgaccaggt
    10921 taccggaaaa ttcgctttcc agcgtgccgt cttccgggcg accgaagtag acgttgtcgt
    10981 gcgcgccata aacgcccaga tctgtaccgt cagcgtaatc tttgtagtaa cgcacacagc
    11041 gatagcaggc gatgcagcgg ttcatttcgt gagagatgaa tggccccaaa tcctgattac
    11101 ggtgggtacg tttggtgaaa cggtagcgac ggaagctgtg tccggtcatc acggtcatat
    11161 cctgaagatg gcagttaccg ccttcttcac ataccggaca gtcgtgcggg tggttggtca
    11221 ttaaccactc gaccacactt tcacggaact gtttcgcttc ttcgtcgtca atggaaataa
    11281 aggtgccatc ggaagccggt gtcatacagg acatcaccag gcgaccacgc gtgtcttccg
    11341 cgttttggta ttgcttcacc gcacactggc ggcaagcacc gacgcttccc agcgccggat
    11401 gccagcaaaa gtaaggaata tcaaggccca gagacagaca agcttccagc aggttgtccg
    11461 ctccgttgac ctcgtattct ttgccgtcta catgaattgt agccattagc atgcttccag
    11521 ttttctcggt cagggaccga gcgttaatcg aaattcggtt accagcgctc tttcagcagg
    11581 ttcggctgaa tcccattaat caaatgggta ttgctgaacg gctgtttgat tcccgcctca
    11641 aattcttcgc ggaaatattt gatggcgctc tgtaacggct ccactgcacc aggtgcgtgg
    11701 gcacagaaag ttttacccgg gcctaagaat cgacacagtt gctcaagtgt ttcgatatcg
    11761 cccggctggc cttcgccacg ctccagcgca cgcagaattt tcacgctcca cggcagaccg
    11821 tcgcggcacg gcgtacacca gccgcaggac tcacgggcga aaaactcttc caggttacgc
    11881 accagcgaca ccatgttgat ctcatggtca accgccatcg ccagcgccgt acccagacgg
    11941 ctgcccgctt taccgatact ttcgaattcc atcggcagat caaggtgcgc ttcggtcagg
    12001 aagtcagtcc ccgcgccgcc tggctgccag gctttaaatt tcagaccatc acgcatacca
    12061 ccggcgtaat cttcgaggat ctcgcgtgcg gtggtgccga acggcagttc ccacagtccc
    12121 ggatttttca cccgaccgga gaagcccatc agcttggtgc cagcatcttt acttttcgag
    12181 atgttctgat accactccac gccgttagcg aggatcgccg gaacgttaca cagggtttcg
    12241 acgttgttga cacaggtcgg tttgccccat acgccggagg ttgccgggaa ggggggtttc
    12301 gagcgtgggt tagcacgacg cccttccagg gagttgatta acgctgtttc ttccccgcag
    12361 atgtagcgcc ctgccccggt atggacgaac agttcgaagt cgaaacctgt tcccataatg
    12421 tttttgccaa gcaagcccgc ttcagtggct tcggcaatgg cgcggcgcag atttactgcc
    12481 gcttcgatat attcgccacg caggaagatg tagccacggt aagctttcag cgcaaacgcg
    12541 gagatgagca taccttccac cagcaggtgc ggcagttgct ccatcaacag gcggtcttta
    12601 taggtgcccg gctccatttc atcggcgtta cacagcaggt aacggatgtt catggattcg
    12661 tctttcggca tcaggctcca cttcaggcca gtcgaaaagc ccgctccgcc gcgccctttc
    12721 agaccagcgt cttttacctg attaacgatt tcgtccggag acagcccggt cagcgcctta
    12781 cgcgcccctt cgtaaccgtt tttgctgcgg tattcgtcca gccacactgg ctgtttgtca
    12841 tcgcgcagac gccaggtcag cggatgcgtt tcgggagtac ggataatgtt tttcatttat
    12901 accgctccag cagttcaggg atcgcttccg gggtcagatg cgcgtgagtg tcctcatcga
    12961 tcatcatgtt tggcccttta tcacagttcc ccaggcagca agttggcagc agcgtaaagc
    13021 ggccatcaaa tgtcgtttgc cctggtttga tgttcagctt tttctcgagc gccgcctgaa
    13081 taccctgata cccgttgata tgacagacca cgctgtcgca ataacggatc acatggcgac
    13141 caaccggctg gcggaagatc tgactgtaga acgttgccac accttcgacg tcgcttgccg
    13201 gaatacccag cacatcggcg atcgcgtgga tcgcaccatc cggcacccag ccacgctgct
    13261 tctgaacgat tttcagcgct tcaatggacg ccgcacgcgg gtcttcgtag tggtgcatct
    13321 cgtgctcaat cgcttcacgc tctgccgcac tcagctcaaa agcctcggtt tgtggttgtt
    13381 gattctcgtg cataattagc ggtccacatc tgacataaca aaatcgatac tgcccagata
    13441 aacaatcagg tcagacacca ggctgccgcg gatcgccgcc ggaatttgct gcaaatgcgc
    13501 ataactcggc gtgcggatac gagtgcgata actcatggtg ctaccgtcgc tggtcaggta
    13561 gtaactgttg atccccttgg tcgcctcaat catctggaaa gattcattgg caggcatcac
    13621 cggcccccac gacacttgca ggaagtgggt gatcagggtt tcgatatgtt gcagcgtgcg
    13681 ctctttcggc ggcggcgtgg tcagcgggtg atccgctttg aacgggcctt ccggcatgtt
    13741 gttgaggcac tgctcaagaa tgcgcagact ctggcgtagc tcttccactt tcagcattac
    13801 gcgggtgtag cagtcagaaa caccaccacc aaccgggatt tcaaagtcga agttttcata
    13861 gccagaataa ggacgcgcct tacgcacgtc gaagtcgatc ccggtagcac gcaggcccgc
    13921 gccagtggtg ccccactcca gcgcctcttt cgcgccatag gcggcaacgc cctgggaacg
    13981 acctttcaga atggtatttt gcagcgccgc tttctcgtaa gacgccagac gtttcggcat
    14041 ccagtcgagg aactcacgca gcaggcgatc ccagccgcgc ggcaggtcgt gcgctacgcc
    14101 gccaatacgg aaccacgccg ggtgcatacg gaaacccgtg atcgcttcca ccagatcgta
    14161 aattttctga cgatcggtaa aggcgaagaa cacgggcgtc attgcgccga cgtcctgaat
    14221 aaaggtcgag atgtacagca ggtggctgtt gatacggaac agttcggaga gcataacgcg
    14281 aatgacgtta acgcgatccg gcacggtgat cccggccagt ttctctaccg ccagcacgta
    14341 aggcatttcg ttaacgcagc cgccgaggta ttcaatacgg tcggtatacg gaatgtagct
    14401 gtgccaggac tggcgctcgc ccattttctc cgcaccacgg tggtggtagc cgatgtctgg
    14461 tacgcagtcg acaatctctt cgccgtcgag ttgtaaaacg atacggaaag ccccgtgcgc
    14521 cgacgggtgg ttcggaccga ggttgaggaa catgaagtcc tcgttttcgg tgccgcgctt
    14581 catcccccac tcttccggtt tgaaggtcag ggcttccatc tccagatcct gtttggcttt
    14641 ggtcagctca aacggcgaga attcggtagc gcgcgccggg taatctttac gcagcgggtg
    14701 acctttccag gtttgcggca tcatgatgcg gcgcaggttc gggtgaccgt cgaaagtaat
    14761 gccaaacaga tcccaggttt cacgctcata ccagttagcg ttcgggaaca gtttggtgaa
    14821 ggtcggtacg tgcaggtcgt tttctgccag cgccaccttc agcatgatgt cgcggttacg
    14881 atcgatagaa atcagatggt agaaaacgga aaaatccgcg gcaggtaacc cttcgcggtg
    14941 tgtgcgcaga cgttcgtcca tgccgtgtaa gtcaaacagc atgacgtaag gtttcggcag
    15001 tttctttaag aaatcgccaa cttccagtaa ttgttcacgc ttgatccaca caacgggaac
    15061 cccggtgcga gtcgcctgaa cagtaaaggc atccggccca aaacggttgc gcagttcgcc
    15121 aattaccgga tcatcaagat gatcgcgggt ctgccaggcg ggttcttgcg cggttaagtc
    15181 ggtcatattg ttcaccattg caaatggtcc gtggtgactg tcggcgcagt aagcttcgcg
    15241 aaatagattt aggaataagc gaagaaaaat ccctttgccg acaggcgcaa attaaatctc
    15301 gtcaggtgta cgcaggttgg ttacggcaat gcgttcaccg cgcttgcgtt cgcgctctga
    15361 ttgcatattg gcgcgataaa cgccctgatc gccaaccacc caagagagcg gacgacgttc
    15421 tttgccgata gattcctgca acagcatcag tgcctgcatg tacgcttcag gacgcggcgg
    15481 gcagcccggg atatacacat caaccgggat gaatttatcg acgccctgca caacggaata
    15541 aatatcgtac ataccaccag agttggcaca ggcacccatt gagataaccc attttggttc
    15601 cagcatctgg tcatacagac gctgaataac cggtgccatt ttggtaaagc aggttcctgc
    15661 aaccaccatc aggtcagcct gacgcggcga agcacgcaat acttctgcgc caaaacgcgc
    15721 cacgtcatgc accgcggtaa acgaagtcac catctcaacg taacagcagg aaagaccgaa
    15781 gttatacggc caaattgagt ttttacgacc ccagttaacc atgtcattga gcttgcccat
    15841 aaacacgttt ttgttaactt cttgctccag agggtcggtt acgatctcct gcttttgcag
    15901 ggggtaacgg tcgttctcac cgttgggatc tatgcgggtg agcgtataat ccatcttaat
    15961 gcctcgcggt tagcgttgac gattagcgat actgttcgtt tccgggttca tacgctcgcg
    16021 gcgtgaacgc gcgggcgtcc agtccagcgc gccaatacgc accagataaa ccagacctgc
    16081 cagtaacaca aaaataaaaa ttgcagcttc cacaaagcct acccagccgc tttcgcggat
    16141 agaggttgac catgcgaaca gatacagcgc ttcaacgtcg aagataacga agaacatggc
    16201 caccagataa aacttggcgg acaggcgtaa gcgggcggag ccgaccgagt cgataccgga
    16261 ttcaaacggc acgtttttcg acctcgcgcg tgcgcgaccg cctaaaaacc aaccgcctac
    16321 cagcattagg caacacaggc caatggcaac gataagaaag atagcgaatg cccagtgatg
    16381 agcgatgact tcagtggttg ttgacatact cattgcttac tcatcaaaag tagcgccaga
    16441 ttcactgctc ttcacggcag atggacgcca catcgattca tggggaggaa taaaaaaaac
    16501 cttacaatca ctgtagaaat tcttttatac agctaattga tgtggtcttt tactcctttc
    16561 tataaccttt tgtcaacttt aacaaaagtt tcttcacatt agtttacata atatcaacac
    16621 cattagcatt taatgccctt tcaccccaga tccttgacga ctccaggata attagatgtt
    16681 gttgaatcgt gtccgttgtg aagcaatgga aaaaatacgg gtctattttg acaggaattt
    16741 gtgtcgattc ctccccccaa aagagagtat tttcttgatc tgtgacacgc ttttgtcatt
    16801 ccataacaaa aacgcagcaa caaatttacg tattttttaa catcattgta gcaggtgatt
    16861 ttttcaggcg attatttgtg cgttcgggac gtgaatctct ggtgattgaa aaatgaacag
    16921 ttttgtacgt tctgcactat gcgaggaagg atttttacta aaaaaaagcc gctggggttt
    16981 aaaacacccc cagcggctcg ttttttacac tattgtctca ggaattatct atcgtccgtc
    17041 gattactcga tatccctttc aatcaacaat gaatcatccc cttccggagc agacattggg
    17101 ctgtattgcc acggattatg gtagctctcc atcgcctgat agatcacctg ggccagctca
    17161 ttattactgg acgggtcata gcacagcaaa tattcggtat caggtagcgg cggtaagcca
    17221 tctactccac tcagcacacg caggtccggg ctcatcatct caaccggcct tgccgtcacg
    17281 ccaagaccgg ctttcactgc cgcacgaacg gccggaagcg tcgaggcgac ataagccagt
    17341 cgccatggaa tatctgcttt attaagcgtt gccagcacca tatcgcgaaa cggacttgga
    17401 tcatccagca atacaagagg gatcggctcg cctttttgca atacgtattc cgctgcgcag
    17461 taccagtgtg ttggcgaggt acgcaggttc aatgccttaa acgctgaagg acgatgggtg
    17521 gttaccatca aatccacttc ctgggattcc agcatttctg ccatataggc attacgttta
    17581 acgcggacat ccagcgctaa tttcggataa accgaactca cgcgatttaa caggaaaggt
    17641 aagatcgtat cggcagattc atctgaagca ccgatagtta acacgccctg aagattactg
    17701 aacattaatg atgagcaggc ctcatcatta aaacgcagga ttttcctggc gtaaccaaga
    17761 agttgaatgc catgttcagt taacagtttg ttgcgaccgt gacgagcgaa cagttctttc
    17821 ccaacgagtt gttccagacg ctgcatttgc tgacttacgg cggactgagt acgacacaca
    17881 gcggcagctg cggcagcaaa agtgttcaga tcggcaacag caacaaatgt tctcagcaga
    17941 tcgaggtcga ggttaattat cggacgattt gcacttatca tatattatca cttactggcg
    18001 gctcatactg agctggttaa tgctgtgcac acacaaacaa gcaattccat ttgtaatgtg
    18061 cctccctggc agtttcatcc ggaacccgga cgaaagtaaa aatgcatatg agttgcacta
    18121 aaaaagcgac tcacattgtt ccgttataat gcctgaagta gatcacagaa tatatcttca
    18181 gggatcgcat atctattaag ttactcactc ttttctattt atgacatgcg cgtgtttgta
    18241 taaatgtaaa tgtgagtcct tgttccactc tcgtgcagca tcgctggtca tacgcgaaca
    18301 cgtacaaaca gcaatggtga ggcggcatca agagcaggga tccgtcattt atgcgagcat
    18361 tttaccccaa aaacctttta tttataaggg ccattgcgaa tcatctgatg caaagttatg
    18421 ttatgttagg caaagtaatc ttctattatt aataagcaca tcaaaacctt tttgaatatt
    18481 aaataataat taattagcat catcctcatt cattaattcc gcttaacaat agtttcacac
    18541 cttgccccca ttacgacacc tcggaaatca tcacgctgtt aaactctccg taatgcggaa
    18601 atatcatcat cgcgtttcat tagtgaattc ttaaatgagg cattttcaca caattatctt
    18661 acagataaaa aaaccagact tacaattaag aatcagaata attcactata taacattgca
    18721 tgtaaagcat atacacctca ttattttgtc attattaagt tattaacagc acaatcgagc
    18781 cttcccctct ggcaaaatct tattctgcag accttcaaaa caccgtcctg ggggagtaca
    18841 ttgttctaag ctgacttcca cggcagggag tggcgataac agcaaaaaag gtcaagattc
    18901 atgtccccca ttgaaaaatc cagcaaatta gagaatgtct gttatgacat ccgtggtccg
    18961 gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa actgaacatc
    19021 ggcaacccag ccccgttcgg ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc
    19081 aacctgccta ccgctcaagg gtattgcgat tccaaaggtc tttactccgc gcgtaaagcc
    19141 atcatgcagc actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc
    19201 ggcaatggtg tatcggagct tatcgttcag gcaatgcagg cattgctgaa cagcggggac
    19261 gaaatgttgg ttcctgcacc agattaccca ctatggaccg cggcggtttc gctttccagc
    19321 ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact ggttcccgga cctcgatgat
    19381 attcgagcta aaattacgcc tcgtacgcgt gggatcgtta ttatcaaccc aaataaccca
    19441 accggcgcgg tatattccaa agagctttta atggagattg tggagattgc acgtcagcat
    19501 aatctcatta tcttcgccga tgaaatttat gacaaaattc tttacgacga cgctgagcat
    19561 cactcaattg cgccgctggc acctgacctg ctgaccatta cctttaacgg actgtcgaaa
    19621 acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac
    19681 gccaaaggct acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt
    19741 cctgcgcaac acgccattca gaccgcgctg ggtggttatc agagcatcag tgaatttatt
    19801 acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt gggaactgat caacgatatt
    19861 ccgggcgttt cctgcgtgaa acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc
    19921 aaacgcttta acattcacga cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa
    19981 gttctgttgg tgcaagggac ggcattcaac tggccgtggc cggatcactt ccgcattgtc
    20041 acgctaccgc gtgtcgatga tatcgagctg tctttgagca agttcgcgcg tttcctttct
    20101 ggttatcatc agctgtaatc ttaatttcac tgccggagat tgcatccggc agcgttatcc
    20161 cgccacaatg acctgatgat gtcatcatac gtaaggtcac tatgaaacag agccattttt
    20221 ttgcccatct ctcccgcctg aaactcatta accgctggcc gctaatgcgc aacgtgctga
    20281 cggaaaatgt gtccgaacac agtttgcagg tagcgatggt cgcccatgcg ctggcagcta
    20341 tcaaaaatcg aaaatttggc ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt
    20401 accacgatgc ctcagaagtg ctcaccggcg atctccctac tccggtgaaa tacttcaatt
    20461 cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc tcagcaaaaa ctggtcgata
    20521 tggttccgga agagctgcgg gatatctttg cgccgttaat tgacgagcat gcatatagcg
    20581 atgaagaaaa atcgctggtg aaacaggcag atgcactgtg tgcatatctg aaatgtctgg
    20641 aagaactcgc ggccggaaat aatgaattct tgctggcaaa aacgcgactg gaagcgacgc
    20701 ttgaagcgcg tcgcagccag gagatggact acttcatgga agtatttgtt cccagcttcc
    20761 atctttcgct cgatgagatt agccaggatt caccgctgta agcagccgga gtctgcgtcg
    20821 catcaggcaa taagcgccgg atgcgacatc aggctcttgt caaaacggaa acagcatcgg
    20881 gatcatcacc acacaaaccg ccatcacgat aatggtgaac ggtaccccca acttcacaaa
    20941 gtcactgaag ctgtaatttc ccggacctaa aaccagtgtg ttaacaggtg aagaaaccgg
    21001 tgtcataaag gcggcggatg ctgccatcgc cacgaccatc gcgaatggat aaggcgacac
    21061 ccccatcgtt ttggcagcag ccagcgcaat cggagccatc aacaccgccg tcgcggtatt
    21121 agagataaat agcccaataa ccgccgacaa gacaaacaaa caccccagca tcatatgtgg
    21181 cccgtaacca ccgccaatgt ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt
    21241 ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa ataatgctcg gccagtgaat
    21301 ggatttatag gcgctttcag catctataca gcggaatttc cccatcagca ggcaggcgat
    21361 gatagcggca acaggattag gaatttcatc tgtcagcatt aacgccacca ttagcaccag
    21421 acagaaaatg gcatggggtg cctggctgtg cgcgggcgat gcttcactca cctcttccgg
    21481 taagttcagc gctacgaagt cgcggccctg tttggccagc ataccgatca gtttccagtt
    21541 acccacaacc aggatgatat cgcccagcag cagaggctca tccgccagcg aaccttccag
    21601 cgccacgcca ttgcgcttta gccccaccac attcagtccg tagcgggtac gaaaaccaat
    21661 ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt aatgaaatct ctgccatgcc
    21721 cacatcaagg gcctggtcag aaaaatactc gccgcgcagt accatcggct ccagcaattg
    21781 ctcactacaa aattgccgga gatcgacatc agccgcagac atatcaataa gcaaaacgtc
    21841 acgcgcgcga aattcagaaa ccccattaac gttcacgata acgcgacgaa aacgccgcca
    21901 gcgttcaaca ccgatgacgt tagcgccata acgctcacgt aatttgagat catccagccg
    21961 ttgaccaatc attggcgatc cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg
    22021 atattcacgg ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg
    22081 ggtatcccct ttcagcatga aacgcatcac taacatatac aaaataccca gcaccagcac
    22141 gaccaggcca acaggtgtta cgctaaagaa actgaagccg tgatagcctt cacgcagcaa
    22201 ttcactgttg actaccaggt tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag
    22261 cccggcaaaa cttaacggca tcatcagacg tgacggcgaa gtttgcatac gcatggcaac
    22321 gcttaacacc acgggaataa agatagcgac aacgccggtt gaactcataa acgcgccaag
    22381 ccccgcgacg gtcagcatca acaaaaccaa cattttgatt tcactgttgc ccgccacttt
    22441 gaccagccat gttcccatta cggtggcaac accggtacgg accaaaccat cgccaataat
    22501 aaacaaggcg gcaatcagga caacgttagg atcagaaaag ccggaaaata cttcggggac
    22561 cgtcagcgtt ccgcttaatg caaacgcgac aataacaaac aaagcgaccg catccatacg
    22621 cactctgccc gtcgcaaaca agacgatggc aacggccagt aatgaaagaa cccaaatcaa
    22681 ttcaccgttc acaacttatc cttgttaatt gagggggatg acttgattct gccataaaaa
    22741 agccccgacg agacggggct aaatcatgat caggtgtttc actgaataat aacatcgcca
    22801 tttagctgtt tggtcacagt aatttgctcc agactgtgga ggaccaaatc gacctcattc
    22861 aggcgcgggg tatctgccgg agcgttaacc gcaatgacat ggcaacccgc cgccaggcca
    22921 gaaagcacgc cagcgggagc atcttccgcc accacacact cctgcggcgc aagccccagc
    22981 agctgcgcac ctaacagata cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg
    23041 gttacaaaca cctctggtgc gggaagccca gctattttat ggcgcgctcg cgctaccggc
    23101 atggagccag aagtcacaat ggcccacgga atacctgctt tattcaaatg attgagtaag
    23161 gcgattgccc ccggaagcgc agtaatacct tcggtttccg tggcctcgat gtgctccaga
    23221 cgcgtaaact cggcggcaat atcagcctcg gatttgcccg ccataaaatg gcgcaaagag
    23281 gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa cccatgacgt
    23341 ctggcccagt tgctccacgc ccgttctacc gcaggcaggg aatccaccag cgttccatca
    23401 agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct caggcgttga taatttgatt
    23461 aatttcgttg gcgctcaaat ggtactgacg cgggcaggca tgccacacat taagcatgcg
    23521 ctgatatttt tcccacattg gcgtctgggc gttaaaacca tgagtcccag cgtcaaaatg
    23581 ggtatagcgc ccttccacat taaccataaa gcggacataa ccgaggtaac gtgcttcagt
    23641 ggcggcgtca aagccgagga aggtgacacg acgttcatcg atggattgct ggtcctgcaa
    23701 attagaccag gaaacatgca acgcatgata catctccata atgtcgatga tagtgcggca
    23761 ggtttcttct ttcagctcgc caaactcgcg atccaattca cgcatctgta atccgtaacc
    23821 acgctcaata attgtttgca ggcgacggta acgttcagca tttgccggat cgagcatagt
    23881 catcatcttg tactgattag acaaaataag acgttgcgcg ttggtcattt ccattgttga
    23941 ctcctgtatc actc
//
LOCUS       DAJTNA010000050        23365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTNA010000050 DAJTNA010000000
VERSION     DAJTNA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23365
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.051"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(77..418)
                     /locus_tag="OO161_001647"
     CDS             complement(77..418)
                     /locus_tag="OO161_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR5462762.1"
                     /translation="MKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRD
                     YGLNLFGMYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSITDCSDRCSDYINPEHKKT
                     IKALQDAGYLK"
     gene            complement(390..4172)
                     /locus_tag="OO161_001648"
                     /pseudo
     CDS             complement(390..4172)
                     /locus_tag="OO161_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001414580.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(4602..7013)
                     /locus_tag="OO161_001649"
                     /pseudo
     CDS             complement(4602..7013)
                     /locus_tag="OO161_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(7010..7696)
                     /locus_tag="OO161_001650"
     CDS             complement(7010..7696)
                     /locus_tag="OO161_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5462763.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7667..8290
                     /gene="tesA"
                     /locus_tag="OO161_001651"
     CDS             7667..8290
                     /gene="tesA"
                     /locus_tag="OO161_001651"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR5462764.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            8280..9089
                     /gene="ybbO"
                     /locus_tag="OO161_001652"
     CDS             8280..9089
                     /gene="ybbO"
                     /locus_tag="OO161_001652"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5462765.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLYSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            9150..10004
                     /gene="cnoX"
                     /locus_tag="OO161_001653"
     CDS             9150..10004
                     /gene="cnoX"
                     /locus_tag="OO161_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5462766.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(10067..10846)
                     /gene="fetB"
                     /locus_tag="OO161_001654"
     CDS             complement(10067..10846)
                     /gene="fetB"
                     /locus_tag="OO161_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5462767.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVRAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(10833..11510)
                     /gene="fetA"
                     /locus_tag="OO161_001655"
     CDS             complement(10833..11510)
                     /gene="fetA"
                     /locus_tag="OO161_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5462768.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            11656..12573
                     /locus_tag="OO161_001656"
     CDS             11656..12573
                     /locus_tag="OO161_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5462769.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12570..13028
                     /locus_tag="OO161_001657"
     CDS             12570..13028
                     /locus_tag="OO161_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5462770.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(13029..13436)
                     /gene="cueR"
                     /locus_tag="OO161_001658"
     CDS             complement(13029..13436)
                     /gene="cueR"
                     /locus_tag="OO161_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5462771.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(13561..14853)
                     /locus_tag="OO161_001659"
     CDS             complement(13561..14853)
                     /locus_tag="OO161_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5462772.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            complement(14856..15788)
                     /gene="glsA"
                     /locus_tag="OO161_001660"
     CDS             complement(14856..15788)
                     /gene="glsA"
                     /locus_tag="OO161_001660"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5462773.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPSVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            16050..18554
                     /gene="copA"
                     /locus_tag="OO161_001661"
     CDS             16050..18554
                     /gene="copA"
                     /locus_tag="OO161_001661"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5462774.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPVPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(18762..19103)
                     /locus_tag="OO161_001662"
     CDS             complement(18762..19103)
                     /locus_tag="OO161_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5462775.1"
                     /translation="MKQATRKPTTPGDILIYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTIVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            19241..20035
                     /locus_tag="OO161_001663"
     CDS             19241..20035
                     /locus_tag="OO161_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5462776.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            20239..20718
                     /gene="ybaK"
                     /locus_tag="OO161_001664"
     CDS             20239..20718
                     /gene="ybaK"
                     /locus_tag="OO161_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5462777.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(20755..22407)
                     /gene="ushA"
                     /locus_tag="OO161_001665"
     CDS             complement(20755..22407)
                     /gene="ushA"
                     /locus_tag="OO161_001665"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5462778.1"
                     /translation="MKLLQRRVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            22625..>23365
                     /gene="fsr"
                     /locus_tag="OO161_001666"
     CDS             22625..>23365
                     /gene="fsr"
                     /locus_tag="OO161_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5462779.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLTAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASIS"
BASE COUNT         5302 a         6568 c         6053 g         5442 t
ORIGIN      
        1 taaataaaga taagtaatta cgcataaaac aacaatgatt ataatagcaa aaataaatat
       61 tatcatcttt gatagattac ttgagatagc cagcatcttg taaagccttt atcgtttttt
      121 tatgctctgg attaatataa tcactacatc tatctgagca atctgttatt gatggacatg
      181 tcaacccatg gtcatttaca gccaagtctt ttttattatc ttcaatcatt tcagaatggg
      241 atagctttac ttttctgccg tacataccga acagatttaa cccgtaatct ctaatctctt
      301 tttcataacc cagccctttc gccgatcggc taacaccagc gtcatttaat ttagacactc
      361 gacaaaatgc catacaatgg aaaaattgat cagtcccacc taaatttaat ctcttcataa
      421 gtatcagata atatatccca tgctctttga tggtttagtt gatcttttct tcttattagc
      481 gctacatctg cgggacttaa ccctaatgga tcaataccat tcaccggatt cagcggatac
      541 gcatacagac tccatccccc ctccagccct atcgggtcct gagtgatata ccgcccctgc
      601 aacggatcgt agtaccggtt ccggttgtag tacagccccg actccttatc atactgctgc
      661 cccggcagcc ggtacggctg gtgcaggtga tgcgggttct cctcattaag ctggttgccc
      721 cattcatcat attcctcgct ccacgccgta ttgccgtctt cactgataag cgccagcggc
      781 aggccccggt ggtcacagtg ataaagatgt atttttcgct caggcaaata aaccggctct
      841 atctgtgccg ccagccgctc caccgtcagc ccgcactgcg caagccactg gcggctttca
      901 ctgctcacgc agtttgcccg gatttccccc tccagcctgt ccagcagccc caccagttca
      961 gccgggaaca ccacaccgtg accgtcttcg ctcccttcct gctgtaactt ctccgccagg
     1021 ctgcggcact gcgctttctc ccgttcaccg ttgtctgttt cgatgcggat gagcggcgcg
     1081 aagctccccg gctggtatac cgtctggata cgcgtggtgt cggtctgtac cgtcgtcagc
     1141 cggtcgccgt cccatccgta ccacgtcacc tccggtttac gcgacagcga catccacccc
     1201 gtcaggtcac gctcgcgacg ccacacccgt ttccccgtcc ggcggctcag cgggtcgtag
     1261 aggtagcggc tctcgaccag tggctcgcca tgctgtatcc gcgtgtggaa caccaggcgg
     1321 tgctggctgt cgtagtggta gtggtgggtc cgctcgtcgt ccgtccgtat cacacccgcc
     1381 gggatgcggt ccgtcttctc cgtcagtctg ccgtattcat catagtggta aacatagtgc
     1441 gcatcctctg cgatgcggtt atccggccac gccgtgagcg tgctgtccgg gtgtagctcc
     1501 gggtccggca gccggtttcc cgccgggtcg gtcgcatacg ggatgcggat gtccaggtcc
     1561 ggtgcgaggg tgcgcacgct ctccagcctg cccgtggcgc tgtagccgta ttcccacgtc
     1621 tgtcgcggac cgctgatgcg caccaggtcg ccgttgtcat tccacccgta gtcacggtca
     1681 tacaccaggc tgttcaggtg ctggctctgt aactggcctg cgggggtgta tgtgctggtc
     1741 agttcatatg cggcattact gcctgccatg ctgccgaagc tgcgcaccgt ctcacggtgc
     1801 agcctgtccc gcgtatactc gaccagcggc gtcccgccca gcttcatgcc cgccaggtaa
     1861 ccgctgccgt aggtcagcca ctccaccggc ggcaggctgt ccggcgtgac gcggtttgcc
     1921 agcccctgtt cgttgtaggc gtgtttcgtc tcatgctgcc acagcagttc ccccgtctcc
     1981 gggttctcca ccgtctgccg ttcgcccgtc aggcggccct tatcgtcgta gccgtagtgg
     2041 acagcgacac ggtggccttc gctcaggtgg ctgatttctc tcagccagcc gtggccgtca
     2101 tactgccact gctctgccgg gtcgccgttc accgtccggt gcgtgatgcg gtccgacgca
     2161 tcgtagtgcc agagggtgac aagcccctca tcctcactct gcgtgagttt tccggtcagg
     2221 tcataatgat aacgttgcgt ccgcccgtca aagccgccct gctgtaccag ccggtccagc
     2281 gcatcgtagc tgaagtcgct gtgactgccg ttctcgttag tcagactgat gacacgtccg
     2341 gcagcatcgt actccatact gcgcgtcagc ccgccctgcg tggtgctgac cgcctttccc
     2401 cacgcatcgt actgtgtctc gctccggttg ccgtccgggg tgataacggc agtcaggtcg
     2461 cctgcggcgt tgtactcata ctgcgtttca cggccctgtg cgtctttcac cgaggttaac
     2521 cggccacggt tgtcatagtg gcggtaaagg ctgatacctt cctcgcggtg aaccgccgtc
     2581 atctgccggg tgctgcccgt cgcatccgtt gtcgtcgccg gtaactcact gtgcgcgtca
     2641 tcgtagcggt agcgtactgt ctccccgctg cgcgatgtct ccgataccag cctgcccggt
     2701 tcatcatatt cccggcggct ctccagcccg tccgggtaca ccaccgccgt cagctggttc
     2761 ccgtcgttat agtaaaattt cgtctcccgc ccgtccggtg tggtgatgtc cgtgatatcg
     2821 ccggacacca cattcagacc gtactctgtc cgccgtcccg ccgcgtccgt ctgcgccgtg
     2881 agccttcctg ccgcgtcata cccgctgcgc gtgacgctgc cgtccgccag ttcttttttc
     2941 accacccgtt tcagcccggc cccgccttct gtatgcagca cctcacgccg gttcaggctg
     3001 tcggtgacgg tgatgcggtc ctgctcataa agatagcggt agcttaaccc tgccgggttc
     3061 agttgctcca ccacccgccc cgtatcgtcg tagcggtagc gcatctccgg ccttcccgcg
     3121 taacggtgcg ccaccatccg gcccaggtgc tgcgcgtcat acgtgaaagc gcgcacctgc
     3181 gtattgctgc ggtcatatac cgccagcagt tcaccggctt ccgtatacgt gtaccgcgcc
     3241 agtggcgcac cgggcaggct ctccgggtat gccgggtcgt gcatcagcca caccgccgaa
     3301 aggcggatac ccctgtcggg gccgtactct gtgctctccg gcagtgaatc aggaatgtcc
     3361 ggtcgttttg ctccggcgac gaccgcctgc tgccgggcct cttccgcccg ctgcgcctgt
     3421 gtggtcagca ccagacggaa ctcccgcccg gcaccgtccg tcacgccggt gatttccccg
     3481 gccaggtcgc ctgcggcctc acgccggtac gtcagcgtcc gcccgaagcg gtccgccatc
     3541 ccggtaagca cccggtacgg cggcagcggc gctggcagta cgtcctcagc acccggcacc
     3601 cgctcagatc accccagtat ccaccacggc ccctgtgcgc tgttggtcgc caggtaaaga
     3661 tgcgggctta accggatatc cggcggcagc gacgcccaca gccgtgccag cgtgtggccg
     3721 tccggctgcg ctgccttacc gccacgcacc agccacattg actcactgcg gctgtacaca
     3781 gcctcccccg gcagcagcga ctcaaagtga atgctccgcc cgccgttgtc gttgagtacc
     3841 agtgcatcat cgcgtatctg taagcggata tcagaaggcg ctttccagcc ggggccaaaa
     3901 attcccactg gcgcaggcgt cttcgtccgg tagctgctgt aggtgcggga gagaatgaac
     3961 ggcagcgggt cgggcagcgc aaggtccgtc tcgccgggct gtacttttgc accgagcagc
     4021 gggttcaccg ggttgcccga ggtcatcccg cccggacaca ccgagcacgc cacgccagtt
     4081 ggcgcgccaa ttcttactcc tgccgaaccc tggacaatgg gaccgccata ctgagtcata
     4141 tctccctgac gcgccgctgg ttttccgctc atataaagtg cccttttagt tattgttcat
     4201 atccctgtgt tagcaacaaa aaaaataaaa tgacacacaa gaccagctaa tgtatttata
     4261 tatttttcac ataaagttat ttgttctcaa tcaacaataa ctgttattgc aggtcacttt
     4321 caatatttta ttaaactaaa atttttagca cagatgaatt catcatcaac aaattcacta
     4381 tatggataat atccattcaa cgcatatgat ttatgatttt attagtctga gttatttttg
     4441 tagggctata tttaaaatta ttaaactcca ccgattcaga ttaatatcag caatagaaag
     4501 ataggaaatc accaattcaa taacatcatc aatgaaacat attgtagaat taatttaaac
     4561 aaggcgttcc attcaaccag aacgcccctt atttactctc atcaccccgc aaactgcctg
     4621 aacagcgcct tacccttaac cagtcgcgca cccagccagc cgccgaaaag cgacagcagc
     4681 agcgcaccac tgcacggcag aacaatccac aatcgccagt ctggctccca cgggaaatca
     4741 aacactttcg cctgcaacac taccagtgcc gtttccgcac caattgcggc caccaggccg
     4801 gaaacaaacc caagcatggc gaactcacac cacaatgtcg tgcgcagcag ttttttcccc
     4861 gcaccgagtg tgcgccatac caccagctcc tgatgacgct ggcgcattcc cacctgcacc
     4921 tgtgccagca acagcaacat accgcaggcg gtgaccagca ccaccatcac ctccagcgcc
     4981 cgacttacct gctccagcac ctgaccgacc tgttttaaaa tcgcgccaat atctaacagg
     5041 ctaatggtcg ggaactggcg gttgagttgc gtcaacatgc cgttgccatt ctcccagcgg
     5101 aaactggtaa gccagctctg cggttgcccg tctaatgccc cttccgggaa aataaaatag
     5161 aaattaggcc gcagactttc ccggtccact ttgcgcaggc tggtcacttt agcgcggaac
     5221 tcctgggtat cgcccataaa agtcacggta tcaccgaggg caacgtttaa gcgtttcgcc
     5281 agcccctctt ccatcgacac ttcatcggct tttggcggcc agttaccggc gacaatcgga
     5341 ttatgatcgg gccgcgtatt ttgctaggta agattgagtt cgcgattaag cgcctcatct
     5401 tcattacctt ctgtcggctt atcgttaatc gccgtcagcc gcgcccgcac taccggataa
     5461 aatgattccg ggactatatg atgttccgcg aggaacactt ttagcggtgc tacctgttct
     5521 gtggcgatgt taattaaaaa gtagttcggg ctttctggcg gtagctgctg ccagcggtcg
     5581 agcagatcgc cacgcaatac cagcaacagc gccagcagca taaaggagag cgaaaatgcc
     5641 gaaagctggc ttaacgttga ccacggctga cgtaacaggc ggctaaccgc caggcgcaga
     5701 ggtagcgatt tcagcgtcat gcggcgaagt acattcagct gcatccagcc cagcacaccg
     5761 cacagcaaag ccagtactac cgcgcccgcc agcaccgccc aaagcagcat gctgccaccc
     5821 attaatccgg cgagcagcag cacaaccacc atactgacaa tcggcagata aaacttcagc
     5881 ggccagacgt tcgctaccac atcattacgt aatacgcgta aaggctgtgt tgccagcaac
     5941 aagcggtatg gtcgtagccc caccagcagc gagatgaccg tcatggtgcc aagcgcccac
     6001 agccacggcc agaggctggc tggcggtagt gcagcaggca gaacgggctt gagcagcacc
     6061 atcaacacgt tttcgaacaa caggcctatg gccccaccgg taacggctga aagcgtcagc
     6121 accatcaacc actgaccgac gattagctta cgcagttgcg ctcgccctgc ccccagcgtt
     6181 ttgaggatcg ccaccagatc gtagcgactg cgacagtaat gattcatcgc taccgacacc
     6241 gctgccactg ccagcagcaa ggtcagaagc gccgaaagca gcaggaactg ttgcgagcgt
     6301 tccatcgatc gccccagcgc gccttcgtcc tgttccagac cgtaccagcg ttgttcgggt
     6361 ttaagctgag gtaacaacca tttctcatag ccgtcgagct ggttctcgtt gccgccgaat
     6421 ttataacgcc aggtgacccg actccccggc tgcacggctc cggttttatc gacatccgcc
     6481 agattcatca tcagacgcgg agccatctgg aaggggttaa aaccggaatc cggttcctga
     6541 atcacttctc cggcaatccg caaggtggca tcgcccacgt caatggtgtc gcccgttttc
     6601 aggtttagca gtgccatcag gcgtggggcc agcaatacgc tgcccgcctg cggtttcagg
     6661 ccagggggat tagtttgcag atcgccatac atcgggtaga tatcatccac cgctttgacg
     6721 ttcgccagct gcggtgtgtc gcctgcaaag gtcattgtgg cgaaagtcag ctgcttgccg
     6781 actttcaggc cgcgcttttg cgcttcctcc agccacgctt gcggcacttc gcgtgaactg
     6841 cgcaacgccc gatcgcccgc cataaactca cggctttgct ggcttaagcc cttctccatg
     6901 cgatcgctga tattgcccag cgccagcacg caggccaccg ccaggcttag cgccagccag
     6961 acaattaata gcgacggcga acgccattcg cgccagaacc aacgtgcaat catgcttcct
     7021 cctgcaactg cccgttcacc agccgtaagc agcggtcgca gcgtgccgcc agttgcaggt
     7081 cgtgggtcac cataatcaac gtggtgccat gttcacggtt gagggaaaac agcaggtcgg
     7141 caattttatc gcccgtctgg cggtcaaggt tgccggtggg ttcgtcggca aacagcacat
     7201 caggtcgacc attaaaggct cgtgccagcg ccactcgttg ctgttcaccg ccggaaagct
     7261 gtgccggaag atgatccaga cgtttaccca gccctaactg ttcgagcaac gctttcgccc
     7321 cgttacgact ttccgcgcta ctctcaccgc gcagcagagc cggaagctcg acgttttcca
     7381 gcgcgttaag ggtaggaatt aacataaatg actgaaaaac aaagccgacg tgcttcgcgc
     7441 gcaactttgc ccgcgcttct tcgtccatat tatgtagcgg ttgtcccacc agactcactt
     7501 cgccactgct gccgtcatca agcccggcga ggatcgccag caaggttgac ttacccgatc
     7561 ccgactcgcc caccagtgcg atggtctcgc cacgtttgac aaccagctca actccggtga
     7621 ggatggagag ttcatgctcc ccctgaccga cggacttctt aagatgatga acttcaacaa
     7681 tgttttccgc tggcatttgc ccttcctgtt cctagtcctg ttaaccttcc gtgccgccgc
     7741 agcggacacg ttattgattc tgggtgatag cctgagcgcc gggtatcgaa tgtctgccag
     7801 cgcggcctgg cctgccttgt tgaatgataa gtggcagagt aaaacgtcgg tagttaatgc
     7861 cagcatcagc ggcgacacct cgcaacaagg actggcgcgc cttccggctc tgctgaaaca
     7921 gcatcagccg cgctgggtgc tggttgaact gggcggcaat gacggtttgc gtggttttca
     7981 gccacagcaa accgagcaaa cgctgcgcca gattttgcag gatgtcaaag ccgccaacgc
     8041 tgaaccattg ttaatgcaaa tacgtctgcc tgcaaactat ggtcgccgtt ataatgaagc
     8101 ctttagcgcc atttacccca aactcgccaa agagtttgat gttccgctgc tgcccttttt
     8161 tatggaagag gtctacctca agccacaatg gatgcaggat gacggtattc atcccaaccg
     8221 cgacgcccag ccgtttattg ccgactggat ggcgaagcag ttgcagcctt tagtaaatca
     8281 tgactcataa agcaacggag atcctgacag gtaaagttat gcaaaaatcg gtcttaatta
     8341 ccggatgttc cagtggaatt ggcctggaaa gcgcgctcga attaaaacgc cagggttttc
     8401 atgtgctggc aggttgccga aaaccggatg atgttgagcg tatgaacaac atgggattta
     8461 ccggcgtgtt gatcgatctg tattcaccag aaagtgttga tcgcgcagca gacgaggtga
     8521 tcgccctgac cgataattgt ctgtatggga tctttaacaa tgccggattc ggcatgtatg
     8581 gccccctttc caccatcagc cgtgcgcaga tggaacagca gttttccgcc aactttttcg
     8641 gcgcacacca gctcaccatg cgcctgttac ccgcgatgtt accgcacggt gaagggcgta
     8701 ttgtgatgac atcatcggtg atgggattaa tctccacgcc gggtcgtggc gcttacgcgg
     8761 ccagtaaata tgcgctggag gcgtggtcag acgcgctgcg catggagcta cgccacagcg
     8821 gaattaaagt cagcctgatc gaacccggtc ccattcgtac tcgcttcacc gacaacgtca
     8881 accagacgca aagtgataaa ccagtcgaaa atcccggcat cgccgcccgc tttacgttgg
     8941 gaccggaagc ggtggtcgac aaagtgcgcc atgcttttat tagcgagaag ccgaagatgc
     9001 gctatccggt aacgctggtg acctgggcag taatggtgct caagcgcctg ctgcctgggc
     9061 gcgtgatgga caaaatattg caggggtgag ttgaagcgcg cgcttaagcc cccatgtcaa
     9121 agaaaatatc gacaacagag agcgactcca tgtccgtaga aaatattgtc aacattaacg
     9181 aatctaacct gcaacaggtt cttgaacagt cgatgaccac tccggtgctg ttctattttt
     9241 ggtctgaacg tagccagcac tgtttgcagt taaccccgat cctggaaagc ctcgcggcgc
     9301 agtacaacgg gcaatttatt ctggcgaagc tggactgcga cgcggagcag atgattgccg
     9361 cccagtttgg tctgcgcgcg attccgaccg tgtatctgtt ccagaacggg caaccggtag
     9421 atggcttcca ggggccgcaa ccggaagagg cgatccgcgc cctgctggat aaagtgctgc
     9481 cgcgcgaaga agagctgaaa gcgcagcagg cgatgcaact gatgcaggaa ggcaattaca
     9541 ccgatgccct gccattgctg aaagacgcct ggcagttgtc gaatcagaac ggggagatcg
     9601 gcctgctgct ggcagaaacg ctgattgcgc tgaaccgttc tgaagatgcg gaagcggtgc
     9661 tgaaaaccat tccgttgcag gatcaggata cccgctacca ggggctggtg gcgcaaatcg
     9721 aactgctgaa gcaggcggct gatacgccgg aaattcaaca gttgcaacag caggtggcgg
     9781 agaatccaga agatgccgca ctggcgacgc aactggcgct gcaactgcat caggttgggc
     9841 gcaatgaaga ggcgctggag ttgctgttcg ggcatctgcg taaagatctc accgccgcag
     9901 acggtcagac gcgtaaaacg ttccaggaga tcctcgctgc gctgggtacg ggtgatgcac
     9961 tggcgtcgaa gtatcgccgc cagctgtatg cattgttgta ttgatattcc gttgttcggc
    10021 acatgaacca caacgccgga tgcgatgctg cgcatccggc atcgcatcat ttcttcttca
    10081 attgcgtcac caccaactgg tggcgcgaat tataaaactt acgataggtt aaatagcagg
    10141 caataatggt cgacaagctg gcggttgaga gcagcataaa ggtcaccata atctgatatt
    10201 taatcgccct caccggatca atcccggcaa atatcagccc ggacatcatt cctggtaaac
    10261 tcactaagcc aaccgttttt gctgaatcga ccgtcggaat taaagccgcg cgaatactgt
    10321 cgcgaatcaa tatcgctgaa gcctgcttcg gcgtcgcgcc aagactcagt ttctcctgaa
    10381 tctgttgctg ttcgctaatg acccgttgcc ctaaattgtt gtaacacaac cccaccgcta
    10441 ccatggcgtt accggcaatc atcccggcga tagggatcac ctgcatcggg ataaattcaa
    10501 tcgaccctga gagaatcagc accgccaggg taattcccgc cccgaccgta atggcaataa
    10561 acgatgagat aaaagcttta gcaatatatt tactgcgttt ttgcgcgttc cacgccgcat
    10621 taaagcagat aaataacacc atcaataatg tcaggctggc atcatccacg ctgaaaatat
    10681 acttcagcac atagccgaca ataatcagct gaattatcgc gcgcccgacg ctccagaaaa
    10741 tatctttctc cagcgccagt ttttctttat ggctaattaa gattgccacc accaccagca
    10801 ttaatgccag tgccaatgat tcgttagtaa tattatgcga gttcatagcg tgcttcctgc
    10861 atttctccgg catgcggttg cagtgtaatc actttatccg catgattaat ttcgtcttta
    10921 tcgtgtgtca cccacagcac ggcaatattt tgctcgcgca cataacgatg gatcatctca
    10981 ttaacgttat gtttattact ttcatccagc gcactggtga tttcatccag caataaaacc
    11041 ttcggcataa attgcaggtt acgaatcagt gagatgcgtt gtttttcacc accagatagc
    11101 tcggcgatat ttttcgtcaa aatgctgtcc ggcaaggcga agcgttcgag aaaatcgaga
    11161 aaaatggctg ggtcaggctg ccggttacgg atctgccagg gaaagatcag attatcgtat
    11221 accgtatcgc caaacagcgt cggtgtctgg gcgcagtaag agacttgttg gcggtagatt
    11281 tctggcttta gtgtgctgac atcctcacct tcaaacagta acgttccgct ggttgggctg
    11341 atcaatgaag caactatttt tagcagcgta cttttgccac aaccagaagg accggtaatt
    11401 aacttaaatt cgccagcacg cagcgaaaaa ttgatgttat taagaatctt cgcatcaccc
    11461 gccagatatc ctacgttttg tagctgaagc aaaggactat tttcctgcat cgctgttccc
    11521 tttttctgat ttttactaaa aacagtttat ccttcgcagg aataaggggg aactctcttt
    11581 cagtaatcag gtataatttg tttaatcaag cagcaatttt gctgattatg gacggtacaa
    11641 caggaggttt ttccgatgct tatctttatc ccgattctca tttttgtcgc gctggtcatt
    11701 gtcggcgcgg gcgtcaaaat cgtgccgcag ggctatcaat ggacggtaga acgttttggt
    11761 cgctatacca aaacgttaca gccggggctc agtctggtgg tgccgtttat ggatcgcatt
    11821 ggtcgcaaga tcaatatgat ggagcaagtg ctcgatatcc cttcccagga agttatctcg
    11881 aaagataacg ccaacgttac catcgacgca gtctgtttta ttcaggtgat tgacgcgcca
    11941 cgcgctgcct atgaagtcag caatctggag ctggcgatca tcaacctgac catgactaac
    12001 atccgtaccg tgttgggttc aatggaactt gacgaaatgc tctctcagcg cgacagcatc
    12061 aactcacgcc tgctgcgtat tgtcgatgaa gccaccaacc cgtgggggat taaagtcacc
    12121 cgtattgaaa ttcgcgacgt gcgcccaccg gcagagctta tctcttcaat gaacgcgcag
    12181 atgaaagcgg aacgtaccaa acgcgcttac attcttgaag cggaagggat ccgtcaggcg
    12241 gaaatcctca aagccgaagg tgaaaaacag tcgcaaatcc tgaaagcgga aggcgaacgt
    12301 cagtcggcgt ttttacaggc tgaagcgcgt gaacgttccg ccgaagcaga agcccgcgcc
    12361 accaaaatgg tgtctgaagc tatcgcctcc ggtgatattc aggcggtgaa ctacttcgta
    12421 gcgcagaaat acaccgaagc gttacagcag atcggttcct ccagtaacag caaagtagtg
    12481 atgatgccat tagaggccag cagcctgatg gggtcgattg ccgggattgc cgaactggtg
    12541 aaagacagcg ccaacaaacg gactcagcca tgatggagtt aatggtcgtt catccacata
    12601 ttttctggct cagtctcggc ggtttgctgc tggcagccga gatgctgggc ggaaatggtt
    12661 atttgttgtg gagcggcgtg gcagcggtga ttaccggcct ggtggtctgg ctggtgccgc
    12721 tgggttggga gtggcaaggg gtaatgtttg ccatcctgac gctgctcgcc gcctggctgt
    12781 ggtggaaatg gttgtcgcgg cgggtgcgcg aacaaaagca cagcgatagt catttaaacc
    12841 agcgcgggca gcagctgatt ggccgacgtt ttgtgctgga atctccgctg gtcaacgggc
    12901 gcggtcatat gcgcgtcggt gacagttcat ggcctgtcag cgccagcgag gatctcggtg
    12961 caggtacgca tgttgaagtc attgcgatag aagggataac cctacatatc cgagccgtct
    13021 cgtcttaatc accctgcccg atgatgacag cagccggaga gattttcgat aatcgggcag
    13081 tcggcgctgt catcgccagg gcaggcattc gccagtgcca gcagctggtc gcgcatggat
    13141 tgcagctcct caatgtgtcg ttcgatctcc gccaccttct ccagcgtgcg ccgtttgacg
    13201 tcggcgctgt gccgctgcgg gtcgttaaac agattcacca gctcgccgct ctcttccagg
    13261 ttaaagccca cctgccgtgc ctggcgcagt aaggtcagtt cgttgagatg ctgctgcgtg
    13321 taggtgcgat aaccgttttc gctgcgcatc ggcggcgtca ccagcccctt ctcttcatag
    13381 aagcgaatgg ctttgctagt caggccggta atttttgcta catcgctgat gttcatcgtt
    13441 cgcgcaacgc ctcctgtgct ttgttaaagg gtttcaacag atattcaagc acgttttttt
    13501 gccagtgcga atatcgaccg ccaccacaat aatacaggaa gtactgcgca cagagagatg
    13561 ttatacggtt ttattgcgct tcatgaccat tgccacaata aggctgagta agagtgagcc
    13621 aataatccat atcaacgcac ggctaccctg ggaccacaga ctgtagataa agccaacgat
    13681 caccaacaac atcaccaacg taccaacgat aagaatcggc aacgaggcgt gaatatcatg
    13741 acgcaggcgg atcgccacca caaacaccgc caggtagcaa atcaaaaagg tggcgctggc
    13801 aacgctggcg agtgagccta aattcagtgc cgccgtcatc agcataatca acaccacgac
    13861 aatgatgttg ccccaggtac tctgccgcca cagggattta ttcattagct tcggcagttc
    13921 gcgttcgctg cccatgttgt ccatgatgtt aaacacggcg aacaggttcg cgttaatggc
    13981 tgaagccgtc gctagtaaag cgccgatgac gacgatcaca taacccacat gtccgagcag
    14041 cggagaagca gcctgcgcta cggcggtatc ggcatatttt tctaactcta atgccgatac
    14101 atcgctaagc aaaaccagtg ccagcgagat ataaagcaac gtggtaacgc caatcgccac
    14161 cagaaacgcc cgtggcataa tgacctgcgg atctttcact ttatccgccg cgttcgccat
    14221 catgccaaag cccgcatagg caaggaaagt tatcccaata caggagaaga acgcaccgga
    14281 gctggggggc gcagagacgg aaatatgcgc tggttgcagc gaccagacac cggcaataat
    14341 cagcaataac aggatcatca ttttaatgcc gacgaggatc acttccagcc gccctaccgc
    14401 atggttgctt aaggagttga aaagcgtcat caccgcaatg ctccccaacg cgtagagcaa
    14461 aataaggtgc tcctcctggc tgccttcatg caaaaactgc acggcataag cgccaaaagc
    14521 atgggcgacc atggcgatgc tcaccgccag cgtcaacagg tacagtaacg agagcgccag
    14581 cgaaaagacg ccgttgccta atccgcgacg aaagaagtcg ataatgccgc cattgctggg
    14641 atagctcgcc cccagacgcg cataggcata accggaaaac atcgccacaa taccgccaaa
    14701 agcaaaggcg acccaggtcg aggcttccat tagcaacgca gcctgcccca gcagcgcgaa
    14761 gatccccgcc cccaccattg ccccaatacc gatggaaacg acattccata gaccgagggg
    14821 tttgttaccg ttattacctt ccgtgttcat catgatcagc ccttaaacac gttatagccg
    14881 agttgcttag cgaccgatgc caccattttt tgaccgcgaa cactgttacc ttcttcgtcc
    14941 agcggtggtg agaacgcggc aattcccatc acgctaggga cgaccgccag aataccgcca
    15001 cctacaccgc ttttgcccgg taaaccaacg cgatacgccc agtcaccgga gcgaccatac
    15061 agcccttcca tcatcatttc ggccagaatg tacggcacgt tgtcggcctg aagaacgcgt
    15121 ttatgcgtca gcggattcag accacccgcc gccagcgtcg cgccaagcgt tgccagttca
    15181 acagtattga tgagcgtgga gcactgacgg gtgtacacgt cacaggcttc cattgcatca
    15241 caatagagat atccggcgga gtacagcagc caggctatgg cccggttatg gaagttggtt
    15301 gtttgttccg actggttgac ttcgtcagag agcgctacct gctcaccagc cagttgctgt
    15361 tggatatgta aaattcgctg ccagcgttgt tcaatatttt cagcgttaat caggctggtg
    15421 gtggcaatag cgccagcatt taccagtggc gaaagcggtt tgccgccatg caactctaag
    15481 gcgataactg agttaaaggg caatccggtc gggtcagcgc caattttgtc ctgtaccgcc
    15541 tgcgggccga catcttctaa cgcaagggct aacgtacaga ctttcgagat ggattccagt
    15601 gcaaagcggt aatcactgtc acccgcacta tagacgttgc catcgcaggt cacgatagcc
    15661 actgccgcca gttgacctgg tacattcgcc agaaagggaa tgtaatcggc attttgtccg
    15721 ccgttaagtg agtgaaattg ggtgtaagcc tgatccactg cctgctgtaa tttgtttgca
    15781 tctaacatct tttgttaact cctttttata gatgcgggag gtaattcctc accccggtgc
    15841 cgattttcag gcatcctgat ttaacttagc accagcaact taactacagg aaaacaaaga
    15901 aataaatgtc taatcctgat gcaaatcgag ccgatttttt aatctttacg gacttttacc
    15961 cacctggttt attaatttct tgaccttccc cttgctggaa ggtttaacct ttatcacagc
    16021 cagtcaaaac tgtcttaaag gagtgtttta tgtcacaaac tatcgacctg accctggacg
    16081 gcctgtcctg cggtcactgc gttaaacgcg tgaaagaaag tctcgaacag cgcccggatg
    16141 tggaacaagc ggatgtgtct atcactgaag cgcacgttac cgggactgcc agtgcagaac
    16201 agctgatcga aaccatcaaa caagcgggtt atgacgcatc tgtaagccac ccaaaggcta
    16261 aaccgctggc ggagtcatca atcccgtcgg aagcactgac agcggtttct gaggcgcttc
    16321 cggcagcgac cgccgatgac gatgacagcc agcagttgct gctgagcggc atgagctgcg
    16381 ccagctgtgt cacccgcgta caaaatgcgc tgcaaagcgt accgggcgtc actcaggcac
    16441 gggtaaacct ggcggagcgt actgcgctgg tgatgggcag tgcctcccca caagatttag
    16501 tgcaggcggt ggaaaaagcg ggctacggcg cggaagcgat tgaagatgac gcgaaacgcc
    16561 gcgagcgcca gcaggaaacc gccgtcgcta cgatgaagcg cttccgctgg caggcgattg
    16621 tcgcactggc ggtgggtatt ccggtaatgg tctgggggat gatcggcgat aacatgatgg
    16681 tcaccgctga caaccgcagc ctgtggttag ttatcggcct gataaccctg gcggtaatgg
    16741 ttttcgccgg cgggcatttt taccgcagcg cgtggaaaag cctgctgaac ggtgcggcga
    16801 cgatggatac gctggtggcg ctgggtacgg gtgtggcgtg gctctattcg atgagcgtca
    16861 acctgtggcc gcagtggttc ccgatggaag cgcggcatct ttactatgaa gccagtgcga
    16921 tgattatcgg tctgattaat ctcggccata tgctggaagc gcgcgcgcgt cagcgttctt
    16981 ctaaggcgct ggagaaatta ctcgatttaa ccccaccgac ggcgcgagtg gtcaccgcag
    17041 agggtgaaaa aagcgtgccg ctggcggaag ttcagccagg gatgttactg cgcctgacga
    17101 ccggcgatcg cgtgccggta gatggcgaga ttacccaggg cgaagcatgg ctggatgaag
    17161 cgatgctgac gggcgaacca atcccgcagc aaaaaggcga aggcgaaagc gtccatgccg
    17221 ggacagtggt acaggacggt agcgtgctat ttcgcgccag tgcggttggc agccatacca
    17281 cgctgtcaag gattattcgc atggtgcgcc aggcccagag cagcaagcca gaaatcggtc
    17341 agctggcgga taaaatctca gccgtatttg tgccggtagt agtggtgatt gcgctgatca
    17401 gtgcagcaat ctggtatttc tttggtccgg taccgcagat tgtctatacc ctggtgatag
    17461 ccaccacggt gctgattatt gcctgtccgt gtgcgctggg gctggcgacg ccaatgtcga
    17521 ttatttccgg cgtcgggcgg gcggctgagt ttggcgtgct ggtgcgggac gccgacgcgc
    17581 tgcaacgcgc cagtacactc gataccgtcg tgttcgataa aaccgggacg ctgaccgaag
    17641 ggaagccgca ggttgtcgca gtaaaaacat ttgctgatgt tgatgaagcg caagcattgc
    17701 gtctggcggc ggcgctggag caaggttcca gccatccgct ggcacgagcg atcctcgata
    17761 aagcaggcga tatgcagctg ccgcaggtca acggtttccg cacattgcgc gggctgggcg
    17821 taagcggtga agctgaaggt catgcgttat tgctgggtaa tcaggcgctg ttaaatgacc
    17881 agcaagtcga tactaaagcg attgaagccg atatttcagc tcaggcatcg caaggagcaa
    17941 cgcctgtgct gctggcgatt gacgggaaag tagtagccct gctggctgta cgcgatccgt
    18001 tgcgtagtga tagcgtggcg gcgctgcaac gcctgcataa agcgggatat cgtctggtga
    18061 tgttgaccgg ggataaccca accaccgcca atgcgatcgc caaagaagca gggattgatg
    18121 aggtgatcgc cggggtgctg ccagacggta aagccgaagc gatcaaacgt ctgcaaagtg
    18181 aaggacgtca ggtggcaatg gtgggcgacg gcattaacga cgcgccagcg ctggctcagg
    18241 cggatgtcgg tattgcgatg ggtggcggca gtgatgttgc cattgaaacc gcggcgatta
    18301 ccctgatgcg ccatagcctg atgggtgttg cagatgcgct cgcgatttcc cgcgcaacgc
    18361 tgcgcaatat gaagcagaac ctgctcggtg cgtttatcta caacagcatt ggtatcccgg
    18421 tcgccgctgg tattttgtgg ccgtttactg gaacactgct taacccggta gttgccggag
    18481 cggcaatggc gctctcgtcg attaccgtag tgagtaacgc caaccggttg ctgcggttta
    18541 aaccgaagga ataagtacgt cattgcggat gcgctgcttt agggcggcgc atttggcatc
    18601 gacgcatgac tgccggatgc ggcgtaaacg ccttatccgg cctacggttc aggcacaggt
    18661 ttgtaggcct gataagacgg gtaagcgtcg catcaggcaa tggtgctcgg atgcggcgtg
    18721 aacgccttat ccggcctacg agatgaagcg atgttttggc cttacgcgac cttttttgca
    18781 cgctcttcac ggcgtgccaa atattcagcc actgtttcaa tccgtcccaa ttcttcttgg
    18841 gtgcgcatgt tgttttcaac ttcccaaaga tcaactgccg cctggaggtt tagccaaaaa
    18901 tctactatgg tatcaaaaac tttcgccaga cggaatgcca tctcagtggt gagtttacga
    18961 ttattattga tcagtgtact gacgctatta cgatgaacat gcagcaactc tgctaactca
    19021 ttgattttca aatcgagcgg ttccagatat tcatagataa gaatatctcc gggtgtcgtc
    19081 ggttttcttg ttgcctgttt catggttttt aactgttgct tccctgtgaa tagcgatgca
    19141 agaacatact gaatcataca aacacattaa acaagtcccc aactccattt gcgccatccc
    19201 tcaccgcgcg ctagcatgat atttcacaaa gggaggtcgt atggatctgt tgtaccgggt
    19261 aaaaacgctt tgggccgcgc tgcgcggtaa tcattacacc tggcctgcca tcgatatcac
    19321 ccttcccggc aatcgccatt ttcatctgat tggcagtatt catatgggta gccacgatat
    19381 gactcccctg cccacccgtt tgctcaaaaa gctcaaaaac gccgatgcgc tgatcgtcga
    19441 ggcggatgtt tccaccagcg atacgccttt tgctaatttg cctacttgcg aggcgctgga
    19501 agagcgcatt aacgaggagc aactccaaaa tctgcagcac attagccagg agatgggcat
    19561 ttctccgtcg ctcttttcta ctcaaccgct gtggcaaatc gcgatggttc ttcaggcgac
    19621 gcaggcacaa aaactggggc tgcgggcaga atacggtatc gattaccagt tattgcaggc
    19681 ggcgaagcaa caacataaac ccgtgattga actggaaggg gctgaaaacc agattaccat
    19741 gttgctccag ctccctgaca aaggactggc gctgctggac gatacgctga cccactggca
    19801 taccaacgca cggttgctgc aacaaatgat gagctggtgg ctgaatgcac ctccgcaaaa
    19861 taatgatata acgctgccca atacgttcag tcagtcgctg tacgatgtgc tgatgcatca
    19921 gcgaaatctc gcctggcggg ataaattacg cgccatgccg ccggggcgat atgtggtcgc
    19981 ggtcggtgca ctacacctgt atggagaagg gaatttgccg caaatgttgc gctaaaaaaa
    20041 tggccaatat cgctattggc ccgtcaaaga ggaatttcat ttttttatta ttatgccgtc
    20101 actttaagcg acggtgtaac tcgattgtcg ctcaatctca ccatcctgcc aatactctta
    20161 ggcaagatga ctcttttttt tggaccgcca tcaggcgtat gcttgcttcg tttttttgtt
    20221 taaggcagga aaattaccat gacacccgca gttaaattac tcgaaaaaaa caagatttcg
    20281 tttcaaatcc atacctacga gcacgatccg gctgaaacca attttggcga tgaagtcgtc
    20341 aaaaaattag gtttgaatcc ggatcaagtc tacaaaacgc tactggtggc agtgaacggt
    20401 gatatgaaac atctggcagt ggccgttacg ccggtcgcag gtcaactgga tcttaaaaaa
    20461 gtagcaaaag cgctgggtgc caagaaagtt gagatggccg atccgatggc cgcgcagcgt
    20521 tcgacgggat acctggttgg ggggattagc ccactggggc agaaaaaacg tctgccgacg
    20581 attatcgacg cccccgcaca agaatttgcc actatttatg tttctggcgg caagcgcgga
    20641 ctggatatcg aactggcggc aggcgatctg gcgaagatcc tcgatgccaa atttgctgat
    20701 atcgcccgcc gcgattaagc attgtgccgg atgcaaatat ccggcacttt cggattactg
    20761 ccagctcacc tcacctttcg gttcataaac actcacatcc agcggcgagc ttttctggat
    20821 atacgctttc agcacttcgg catcaataaa gccggtattc acatagcccg gtttgttatc
    20881 aaggcgcgga tagccatcac cgccggtggc attgaagttt aatgtcgcca tacggtaagt
    20941 tttcgccgga tcgaccggtt cgcctttgat tttcaggtcg ttgagtttgc cgtctttcgt
    21001 cacaaagcta acgttggcaa attgcgggta ggcacctgaa tctggcttca tttgtgcgac
    21061 ggcggtcagg taatcaatca cctctttacc ggtcatgtcg gcatacacca ccacattgcc
    21121 gaatggctgc actttcagta cgtttttata gctgatatcg cctgcttcga tagaatcacg
    21181 aattcctcct ccactcatca ccgcaaagtc ggcaccagtg cgatccattt gggctgccag
    21241 aatcaaccgc cccatattgg tctgtacaaa acgcactttg tcacgatcgc cttccagacg
    21301 accattggtt tcgcctattt tcacttccaa ctgcgctttg cctttgttct ggaacggcga
    21361 taacagcgag atcatttgct ggttttcagc gatttcagga gtgtaaagca cgcgctcgct
    21421 tttcccgtct tcccaggtca ctttcttctt caggttcacc ggaatcagct ggtagttaac
    21481 cattttcatt tcgccattac gaaactcaaa atcagcccgt cccacgtatt tgccccactc
    21541 atgcgcctgc acaatccaga tgccgttttg ttgatctggt ttgcatggcg tacccggcac
    21601 gtaatcgacc tgttttttgt tttctgccgc catgcagacc ggagcttgcg agtgaccacc
    21661 gacgatcatc gccagcgatc cggcaggcag cgcgcgtgcc atctccacat cgcccggtgc
    21721 gttagagccg tgctcaccat tatcgtaatg ccccatatgg gtcgccgcga taataatgtc
    21781 tggcttttct gtctgttgca gctcctgaat caccagcttc gcttcatcgg cgggcttacg
    21841 aaattcgaca tcagtgaagt attccgggtt accaattttt gctgtgtcat cggttgtcag
    21901 cccaatgacg gcaattttca gatcctgacg cttaaacagc gcccatggtt taaacaggcg
    21961 ctcgccggta cttttctggt agatattcgc ggaaagcaac gggaacttgg cccacttttc
    22021 ctgctggcgt aatacggtga gcggattatc aaattcatga ttaccgatcg ccatcgcgtc
    22081 atagcccacc agattcatac cgcgaaaatc aggttcggca tcctgtaagt cagactcggg
    22141 cacgccagtg ttaatgtcgc caccggaaag tagcagcacg ctaccgcctt cagccgcaac
    22201 ctctttgcgg ataccatcca ccagcgtttt ttgcgccgcc agaccatatt cgccatattc
    22261 attgcgccaa aaatgcccat gatgatcatt ggtatgcaga actgtaattt tgtaggtttt
    22321 atcctgctca tacgccagag cagtttcact cgccagtgta aatgtggtta acagcgctaa
    22381 cgccacgcgc cgctgcaata atttcatact tctctccctg acctgatttc aacgtgttga
    22441 aaaatagtat gacgcaacat catagacaag tctgcgtccg gtgaagataa atctgcatcc
    22501 agattagaaa ttctgtattc ggcgttgaga cttccttcag gcgacgcata caggtatgtt
    22561 agtcgggtaa taatcacaaa taagatcatt ctttacatcg caaccctgaa aaagtgactt
    22621 tcctatggca atgagtgaac aaacccagcc tgtggcgggc gcggctgcgt caacgaccaa
    22681 ggcccgaaca tcgtttggta ttttaggtgc tatcagcctc tcacatctgc tgaacgacat
    22741 gatccaatcg ctgattctgg cgatttatcc tctgcttcag tcagaatttt ctctgacatt
    22801 tatgcagatt ggcatgataa ccctcacctt ccagctcgcc tcttcgctac tgcaaccagt
    22861 ggtcggctac tggaccgata aatatccgat gccgtggtcg ttgccaattg gcatgtgctt
    22921 taccttaagt ggtctggtgc tgcttgcgct ggcgggcagt tttggcgcag ttctgctggc
    22981 ggcggcgctt gtcggtaccg gttcatcggt ctttcatccg gaatcttctc gcgtagcccg
    23041 tatggcttcc ggcgggcggc atggcctggc gcaatctatc tttcaggtcg gcggcaactt
    23101 tggcagttcc ctgggaccct tgctgacggc ggtgattatc gcgccttatg gcaaaggcaa
    23161 cgttgcctgg tttgtgcttg cggcactgct ggcgatcgtg gtgttggcgc aaattagccg
    23221 ttggtactcg gcacagcacc gaatgaataa aggaaaaccc aaagcgacga taatcaatcc
    23281 actgccgcgc aacaaagtgg tactggcagt cagcattctg ttaatcctca ttttctcgaa
    23341 atatttctat atggcgagca tcagc
//
LOCUS       DAJTNA010000051        23090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTNA010000051 DAJTNA010000000
VERSION     DAJTNA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23090
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.052"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..198)
                     /gene="pspE"
                     /locus_tag="OO161_001667"
     CDS             complement(<1..198)
                     /gene="pspE"
                     /locus_tag="OO161_001667"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5462780.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVY"
     gene            complement(273..494)
                     /gene="pspD"
                     /locus_tag="OO161_001668"
     CDS             complement(273..494)
                     /gene="pspD"
                     /locus_tag="OO161_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5462781.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(503..862)
                     /gene="pspC"
                     /locus_tag="OO161_001669"
     CDS             complement(503..862)
                     /gene="pspC"
                     /locus_tag="OO161_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5462782.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(862..1086)
                     /gene="pspB"
                     /locus_tag="OO161_001670"
     CDS             complement(862..1086)
                     /gene="pspB"
                     /locus_tag="OO161_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5462783.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1140..1808)
                     /gene="pspA"
                     /locus_tag="OO161_001671"
     CDS             complement(1140..1808)
                     /gene="pspA"
                     /locus_tag="OO161_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5462784.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            1975..2952
                     /gene="pspF"
                     /locus_tag="OO161_001672"
     CDS             1975..2952
                     /gene="pspF"
                     /locus_tag="OO161_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5462785.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHLGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3072..4337)
                     /gene="puuE"
                     /locus_tag="OO161_001673"
     CDS             complement(3072..4337)
                     /gene="puuE"
                     /locus_tag="OO161_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5462786.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENALKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4375..5655)
                     /gene="puuB"
                     /locus_tag="OO161_001674"
     CDS             complement(4375..5655)
                     /gene="puuB"
                     /locus_tag="OO161_001674"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5462787.1"
                     /translation="MTEHTSSYYAASANKYVPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTAPMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFHAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(5657..7144)
                     /gene="puuC"
                     /locus_tag="OO161_001675"
     CDS             complement(5657..7144)
                     /gene="puuC"
                     /locus_tag="OO161_001675"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5462788.1"
                     /translation="MNFHHLDYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7419..7976)
                     /gene="puuR"
                     /locus_tag="OO161_001676"
     CDS             complement(7419..7976)
                     /gene="puuR"
                     /locus_tag="OO161_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5462789.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAICTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTITGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8003..8766)
                     /gene="puuD"
                     /locus_tag="OO161_001677"
                     /pseudo
     CDS             complement(8003..8766)
                     /gene="puuD"
                     /locus_tag="OO161_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            8978..10396
                     /gene="puuA"
                     /locus_tag="OO161_001678"
     CDS             8978..10396
                     /gene="puuA"
                     /locus_tag="OO161_001678"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5462790.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLYGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQILLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10545..10709
                     /locus_tag="OO161_001679"
     CDS             10545..10709
                     /locus_tag="OO161_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462791.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPSPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            10699..12084
                     /gene="puuP"
                     /locus_tag="OO161_001680"
     CDS             10699..12084
                     /gene="puuP"
                     /locus_tag="OO161_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5462792.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12218..12463
                     /locus_tag="OO161_001681"
     CDS             12218..12463
                     /locus_tag="OO161_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5462793.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            12776..14419
                     /gene="sapA"
                     /locus_tag="OO161_001682"
     CDS             12776..14419
                     /gene="sapA"
                     /locus_tag="OO161_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5462794.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLHR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14416..15381
                     /gene="sapB"
                     /locus_tag="OO161_001683"
     CDS             14416..15381
                     /gene="sapB"
                     /locus_tag="OO161_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5462795.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15368..16258
                     /gene="sapC"
                     /locus_tag="OO161_001684"
     CDS             15368..16258
                     /gene="sapC"
                     /locus_tag="OO161_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5462796.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16258..17250
                     /gene="sapD"
                     /locus_tag="OO161_001685"
     CDS             16258..17250
                     /gene="sapD"
                     /locus_tag="OO161_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5462797.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKVNVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17252..18058
                     /gene="sapF"
                     /locus_tag="OO161_001686"
     CDS             17252..18058
                     /gene="sapF"
                     /locus_tag="OO161_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5462798.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18126..18479
                     /locus_tag="OO161_001687"
     CDS             18126..18479
                     /locus_tag="OO161_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5462799.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            18847..19635
                     /gene="fabI"
                     /locus_tag="OO161_001688"
     CDS             18847..19635
                     /gene="fabI"
                     /locus_tag="OO161_001688"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5462800.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            19780..20907
                     /locus_tag="OO161_001689"
                     /pseudo
     CDS             19780..20907
                     /locus_tag="OO161_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            20975..22909
                     /gene="rnb"
                     /locus_tag="OO161_001690"
     CDS             20975..22909
                     /gene="rnb"
                     /locus_tag="OO161_001690"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5462801.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(22987..>23090)
                     /locus_tag="OO161_001691"
     CDS             complement(22987..>23090)
                     /locus_tag="OO161_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462802.1"
                     /translation="TYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
BASE COUNT         5199 a         6129 c         6016 g         5746 t
ORIGIN      
        1 atacactttc acggtgtcgt ttttatccgg aacggcggtg gcaatgcgct ctttcacttc
       61 cttcagggga atattgatgg ccccctgaac gtgctcttgc tgatactgct ctggaacacg
      121 aacatcgatc cagtgttcag cggcgaaaac gggcagtgaa aacaccagtg ccagagcaag
      181 taagcctttt ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg
      241 cctgagattt aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta
      301 ttagcagccc gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg
      361 cgagcaactg attttatcgc ccagcccgct acacccgccg ggccatagcg cagtgcggta
      421 agaagtacca gcttgcctgc taatttgaaa ccaggcttta ccttttgccc ggcctgttgc
      481 cagcgagtat tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat
      541 cggaagtgac ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt
      601 cgacttcatc cagcaattcg ctgctggagg gtagctgctc accaaaggcc atgttgtccg
      661 gcattggatc aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac
      721 cgaagaaaat cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt
      781 tggcaatccc ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataattttt
      841 tattgagatt aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat
      901 agattccagc gcctgaatac gttcgcgcat ccgttttgct tcatcagcca gttgcgctaa
      961 tcgctgctgc tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca
     1021 taaccagatc ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc
     1081 gctcataagt actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat
     1141 tattgattgt cttgcttcat tttggctttt aattgtgcca gttgttcact gattgcatca
     1201 tcggctttca gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt
     1261 tctgcttccg cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct
     1321 tcatccagtt tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga
     1381 tggcgtaaca tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctca
     1441 ccaatctctt tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccaga
     1501 ctcttaatca gatcggtcag tttctgtttt tcaattaacg ccgcacgtgc caaatcctct
     1561 ctctctttca gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct
     1621 tgttcaatac ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt
     1681 acttcaacca gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct
     1741 tccgctttct ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa
     1801 atacccataa tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat
     1861 acaatttgcg tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcatc
     1921 agcaacatgc caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggca
     1981 gaatacaaag ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt
     2041 tcgcatctcg caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa
     2101 gagctgattg ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc
     2161 cttaactgcg cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg
     2221 ggggcgttta ccggtgcgca aaaacgtcat ctagggagat ttgaacgtgc cgacggcggt
     2281 acgctatttc ttgatgaact cgctacggca ccgatgatgg tgcaggagaa attattgcgc
     2341 gtgattgagt acggtgaact ggagcgcgtt ggcggcagcc agccattgca ggtgaatgtg
     2401 cggttggtat gcgcgacgaa tgccgatctc ccggcgacgg tcaatgaagg cacttttcgc
     2461 gctgacctgc tcgaccgact ggcttttgat gttgtacaac tgccaccact gcgcgagcgc
     2521 gaaagcgaca taatgttgat ggcagaacac tttgccatcc agatgtgtcg ggaaatcaag
     2581 ctgcctctgt tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg
     2641 ccgggaaata ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc
     2701 agcgattatc cgcttgatga catcattatt gatcccttta aacggcgtcc gcctgaagac
     2761 gctatcgccg tttcagaaac cacctcgctt ccaacactgc cgctggattt acgtgagttt
     2821 cagatgcagc aggaaaaaga gttgctgcaa ctcagtttgc aacaagggaa atataaccag
     2881 aagcgcgcag ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag
     2941 caccagattt agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc
     3001 ggcatacata atcaataagt cgtttgcagg cctgataagc gcagcgcatc agacattatt
     3061 gcgttgggct attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc
     3121 gcatccggga tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc
     3181 agcaggagca gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac
     3241 ggctcgcccg tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc
     3301 gcagcaatgg ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc
     3361 agttgattcg cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg
     3421 gcagccaccg ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg
     3481 tccataatat tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc
     3541 gccatcgtca ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta
     3601 cgcgcaaagc cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg
     3661 cggcgaatgg cggcaaccag ctcttttggc gcaacgttga aaccgccctc gccctgcacc
     3721 ggttcgaaaa taatggccgc cacctgcttc gcttcaatgt ccgatttaaa caagcgttcg
     3781 atggcgtcga gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga
     3841 tacaccgaac cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc
     3901 gccatggtca tatatgtacg accgtggaag ccgccgctaa acgcaatcac gccaggtcgt
     3961 ccggtatggg cgcgagcaat tttcaacgcg ttttccaccg cttccgcgcc ggtagtgaag
     4021 aatgcggttt tagcctgccc gctcactggg gcaagggcgt taattttctc cgccagcgtg
     4081 acgtagcttt cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc
     4141 tccaccgccg cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg
     4201 gcgaaatcga tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg
     4261 gcgaagaagt tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg
     4321 aattcattgt tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac
     4381 gcccagacga tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag
     4441 cgtgcgcccg ccggggaacg ggtaatgcgg cagattggcg aaggcatgga aacgctcggc
     4501 gtcaccgcgc agcagttcag caatcaaacg cccggcgagg tgggtacagg tgacgccgtg
     4561 gccgctgtag ccctgcatgt aatagatgtt ggtatcgagg cgaccgaact gcggcatccg
     4621 tgaaagggtc agcaggaagt tgcccgtcca gcgatagtcg attttgacgc ccttcagctg
     4681 cgggaaggtt ttcagcagtt tcggcacaac aagacgctca acgtcatccg ggtcgcgcgc
     4741 gccgtagacc acgccgccgc cgtacagcaa acggttgtcg gcggtaagac ggtagtaatc
     4801 aagcagataa ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc
     4861 ttccgacagc cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgccag
     4921 ttccggctct actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt
     4981 cacctgacct ttggcagtgc gcacaacggc tggcgtggtg tgctggatct gcgtcacggc
     5041 agaaagttca tacacgcgcc cgccgttgag gcggatggcg tccgcttcgc caatggcgag
     5101 gtttagcggg tgaatatgcc caccgctgtg atccagcagc gcaccggtat agcgatcgct
     5161 ggctacttca cggcgaatgg cgttggcgtc cagcaattcc agctgtttat tgccgtagcg
     5221 ttcccagttc tctttctgct cttcaagtgt ggcgagctgt ttatcattca tcgccacaaa
     5281 caggccgccg gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat
     5341 ctcaccacct tcgaacatca tgctgccgag cataggggcg gtgtccatgc cgtagctttt
     5401 ttcgatcacg tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct
     5461 tgcgccaaag ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag
     5521 atgcagcgcg gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat
     5581 tgactcattc agggtgtcga atggcacgta tttattcgca ctggcggcgt aataactgct
     5641 ggtatgttcg gtcatttcag gcctccaggc ttatccagat ggttttcagt tcggtgaatt
     5701 tttcaagggc atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccaccaaacg
     5761 gcacggtcat atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcggc
     5821 ggctcatacg gtgcgcgcgg gagaggtcgc gcatccatac cgccgcgcca aggccgtact
     5881 ggctgtcgtt ggcaagctgt agcgcctgtt cttctgatgt gaaacgcgtg accaccagca
     5941 ccggaccgaa aatctcttcg cggcttaagg acgcattcgg gtctacatcc acaaagatgg
     6001 tcgggccgat ggcggcagcc agcccggcgt tacggccatc cagcagcagt tgccctttgc
     6061 cttcgccctc acgaataaag ctatggaccg agtcggcgtg ggcgcagtcg attaaagtgc
     6121 ccatggtggt tgcggggtca agcggatggc ccggctgcca gttttgcgcc tgctgtttta
     6181 acagggctaa gaattcatcg gcgatgctct cttccagcaa caggcgtgtt ccggcgatgc
     6241 acacctgacc ctggttgtag aagatgcctg ctgcggtgga gctcgccgct ttttgcagat
     6301 ccgaacaatc ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacgcgtt
     6361 tcatgttgct gtcgcccgca tctttcagca gctgtttccc ggtacgggtt gaaccggtaa
     6421 aggcaatggc gtcgatatcg ttatgacgcg acagcgcctg ccccgcttca tgaccaaaac
     6481 ccgtcaccac gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac
     6541 gaatcgcact gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca
     6601 gcgccgggcc gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg
     6661 caatcacgcc gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca
     6721 cttcgccata cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg
     6781 gaatatcatc acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg
     6841 ccagctcttc ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt
     6901 tagccggaga agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg
     6961 cacggtcgat atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg
     7021 gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata
     7081 agcgattttc aatggcgaga cttaacgctt tatcctgcca gtaatccaga tgatgaaaat
     7141 tcattatgac tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca
     7201 gagctccccg ggccgtatgc ccgtttattt ctggcgacat tctttatata cacttcatcc
     7261 ttcaagccgc ctctgccttg gctgcgttcg ctcccccgaa tcacttacct gagtaagctc
     7321 atcgggattc gctcacttgc cgccttgatg caacttgaat gattttgtgt atagattgcc
     7381 gggcgcattg cgcttacccg gcttacagaa caaaaagatt aaaacgtggt gggcgtatgg
     7441 gcgctgataa ttcggcaaat acctgccgac gtattactga aactgtgcgg gatgccggta
     7501 ttaatggcat aactttgacc cgcgacgagg tggtaatcct gaccattaat cgtcagaaca
     7561 atttcacctt ccagtactgt gcctatttcc tcaccctgat gcttaattct ttccccagtg
     7621 attgtgcccg gctggtacgt ttcaaagatc atcgccagcg tgcgattcgg gttaccgtta
     7681 tgaaccagct tcattgacac accctgactg cccatctcaa ttaagtcgtc ctgattaatg
     7741 acgacctgcg gctcatcagg tttttccggc tcggaaaaga attccgagag tgacagacca
     7801 tacaccttca gcagcttttg cagcgtactg atggcaggac tgactttatc ttgttctatc
     7861 gtacagatag cactgtgagt cagcccggag agttcggcgg cacgacgttg tgaaagcccc
     7921 tgctgctggc ggatttccga caagcgtttt cctggcgcca gtccctcatc actcatattt
     7981 gcatttcctt aaactgtagt ggtcagagtc gctgtttttc agcgatatgg tgctgacaag
     8041 cggtgataaa gccctcgaac aatatacgcg aaagcgcgta ctcgctactg ttccattccg
     8101 ggtgccactg tacgcccagc gcaaaaggat gattgatgac gctaaccgcc tcaactaagc
     8161 catccggcga gcgagcttca acacgcaacc gtgggctaac gaccttcgcc ccttgtccat
     8221 gtagagagtt tacccaaaag ttgctacatt caggtaacaa cgcagacagt aatcccccct
     8281 cttcaacctg aacttcatgc gacggtgcat attgctgttc caccggcagt tcggatcttc
     8341 ccgatgttcc agcaattcag gctgttcgca cagcttgcga tgcaacgaac caccggttgc
     8401 caccaccagt tcttgtaaac cccggcagat ggcgaaaatg gggatgcgcc tttcgagtgc
     8461 ggcattaatt atcgccatgc tcagaagatc acgcccggga tcggcgtcag gctcatcgcc
     8521 gttttcacca tataggtgcg gctgcacatt gctgggacta ccaggaagat aaatgccatc
     8581 gagtttcggc aaaagttgtt caagtaacga cggttccgcc agcgcatgtg gtagcgcaat
     8641 tggcaagccg cctgcgtgga tgatggcatt caggtacttt tcttgcagag tctgggtcgc
     8701 atgaccctta agcctgttcc tgcacattac gacaccgata accggattgt tcattatatt
     8761 ttccatgctc aatctcacaa agtggactaa attatcgcca ttactgcgct ttaaatgacc
     8821 tttatgttca atattttttc aatctagcag ttggttttca tttttgcaaa ctaaatttaa
     8881 catttgacga acatttagtt tgcatacaga ttcgaatggt ggtcattata ttttacgctt
     8941 tgataacgag cggaaaacaa accaaaggcg aagaatcatg gaaaccaata tcgttgaagt
     9001 agagaacttt gttcagcagc cagaagagag gcggggtagc gcctttacgc aggaagtgaa
     9061 acgctacctg gagcgctacc cgaatacgca atatgttgat gttttactta ctgatttgta
     9121 tggttgcttc cgtggaaaac gtatccctgt ttcaagcctg aagaagttgg agaaagggtg
     9181 ttatttcccg gcctcggtgt ttgcaatgga tattctgggc aacgtagtag aagaagcggg
     9241 tctgggtcag gaaatgggcg agccggatcg tacttgtgtt cctgttctcg gttccttaac
     9301 cccttctgcc gccgatccag agtttatcgg tcagattctc ctgaccatgg tcgatgaaga
     9361 tggcgctccc tttgacgttg agccgcggaa cgttcttaac cgcctgtggc agcagctacg
     9421 ccagcgcgga ttgttcccgg ttgtagcggt agagctggag ttctatttac tggatcgtca
     9481 gcgcgacgct gaagggtatc tgcaaccgcc ctgcgcgccc ggcaccgatg accgcaatac
     9541 acaaagccag gtttactccg ttgataacct caaccacttc gccgacgtgc tcaatgatat
     9601 tgatgaactg gcgcagttac agttaattcc cgccgacggt gcggtcgccg aagcctcgcc
     9661 gggtcagttt gaaatcaacc tttaccatac cgataacgtg ctggaagcct gcgatgatgc
     9721 gctggcgcta aaactcctgg tgcgtctgat ggcagaaaag cataagatgc acgccacttt
     9781 tatggcgaag ccgtatgaag agcacgcggg cagcgggatg catatccata tcagtatgca
     9841 aaataatcgt ggcgagaatg tgctttctga cacagaaggc gaagattcgc cgctgctgaa
     9901 aaagatgctc gccgggatga ttgacctgat gccgtcgtcg atggcgttgc tggcaccaaa
     9961 cgtgaactcg tatcgccgct tccagccggg aatgtatgtg ccgacgcagg cgtcgtgggg
    10021 ccataacaac cgcaccgtcg ccctgcgtat tccgtgcggc gaccgtcata atcaccgcgt
    10081 ggaatatcgc gtggcgggtg ccgatgccaa cccatatctg gtgatggcag cgatttttgc
    10141 cggtattttg catggccttg ataacgagct gccgttgcag gaagaagtcg aaggcaacgg
    10201 gctggaacag gaaggcttac ccttcccgat tcgccagagc gatgccctgg gtgagtttat
    10261 cgagaatgat cacctgcgcc gctatttagg cgaacggttt tgccatgtgt atcacgcctg
    10321 taaaaatgat gagctgttgc agtttgagcg cctcatcaca gaaacagaaa ttgagtggat
    10381 gttgaaaaac gcatagtacc ccttgcgggg ttgtttcatc gacctgagcg gagccaggcc
    10441 tgacatttgc tggtaatcta gacatttccc ggatgtcacg ttatggagcg caggcggcaa
    10501 cgggcttccg ttaacgatta agactgtgac gaggaaatga gatgatgccg atgattaaat
    10561 ccccgcatgg cgagggaggc tgcgtatgcg cccctcctgc tactgactgg actccgtccc
    10621 ctttgcttcc cttgctaaac aggtttgatt tcaggtcgac acgaccgcaa accttattac
    10681 gcaggggagg cagcaattat ggctattaat tcaccactga atattgctgc gcaacccggc
    10741 aaaacccgtc tgcgaaaatc actgaaattg tggcaggtgg tgatgatggg tctggcctat
    10801 ctcacgccga tgaccgtatt tgatactttt ggtattgtct ccggcattag cgacggtcac
    10861 gttccggcgt cctatttgct ggcgctggcg ggcgtgctgt ttaccgctat cagctacggc
    10921 aaactggttc gccagtttcc ggaggccggt tcggcctata cctacgcgca aaagtcgatt
    10981 aacccgcacg tcggatttat ggtcggctgg tcatcgctgc tggattatct ctttttgccg
    11041 atgatcaacg tcttgttggc gaaaatctat ctctccgccc tcttcccgga agtgccgccg
    11101 tgggtgtggg tagtaacctt cgtcgccatt ttaaccgccg cgaatctgaa gagcatcaac
    11161 ctggtcgcta acttcaatac cctgtttgta ctggtgcaaa tctccatcat ggtggtgttt
    11221 atcttcctgg tagttcaggg actgcataaa ggagaaggcg ttggcaccgt ctggtcactt
    11281 cagccgttta tcagcgagaa cgcgcacctg atcccgatta ttaccggggc gacgattgtc
    11341 tgtttctcct tcctcggttt cgatgcggtg accacgcttt cggaagagac gccggacgcc
    11401 gcacgcgtga tcccgaaagc catcttcctg acggcggtct acggtggcgt tatctttatc
    11461 gcggcgtcgt tctttatgca gctgttcttt cccgatatca gccgctttaa agatccggat
    11521 gccgcactgc ctgaaattgc gctgtacgtc ggcggcaagc tcttccagtc gattttcctc
    11581 tgcaccacgt ttgtgaacac gttagcctct ggcctggctt cacatgccag cgtgtcgcgt
    11641 ctgctgtatg tgatggggcg tgacaatgtg tttccggagc gcgtgtttgg ctatgtgcac
    11701 ccaaaatggc ggactccggc actcaacgtc attatggtcg ggattgtcgc gttgtcggcg
    11761 ctgttcttcg atttagttac cgccacagcg ttaattaact tcggtgctct ggtggcgttt
    11821 accttcgtga atctgtcggt gtttaatcat ttctggcggc gtaaaggaat gaataaaagc
    11881 tggaaggatc acttccacta tttgctgatg ccgctggttg gcgcgctgac ggtgggtgtg
    11941 ctgtgggtta acctcgagtc aacgtcactg acactcggtc tggtatgggc ttcgttaggc
    12001 ggcgcatatt tatggtatct gatccgccgc tatcgcaaag tgccgttgta tgatggtgac
    12061 agaacgccgg tgagtgaaac gtaatagtcg gatgcgcggc gcattatcct gcctgagggt
    12121 aatgcaccgc gccattaact ataaaaagat gtatgacccg gatcacgagt gctatcatcc
    12181 gcccttatgc ttagcgcatt aaccccaggg gcaaataatg aaccacgata tcccgctaaa
    12241 atattttgat attgccgatg agtacgcgac cgaatgcgca gagcctgtcg cagacgctga
    12301 acgcacaccg ctggcccact acttccagtt actgctcacc cgtttgatga acaatgaaga
    12361 gatcagcgaa gaagcccaac atgaaatggc tgctgaagcg gggattaatc ccgtacgcat
    12421 tgatgagatc gcagagtttt tgaatcaatg gggtaatgag taattattta attgcagacg
    12481 atcggtcagt atcgttgtcc ggataaagcg ttcacgccgc atccggctaa caatgacacg
    12541 ctctgcagcg tgctttaatg ggtttctcac gatcgtaccg ctctgctcct cgctaagttg
    12601 ttctgtaagt tatagttata gagaaagcac gggtacgaat gttgaacaga tggcgacgcc
    12661 acctgttcaa cgaccaacca cacggccctc attacgaccc ctgaacacaa acggtaatgg
    12721 caaatgtcat taaatattag taagatagcc tctttacata aaatccctta atattatgcg
    12781 ccaggtatta tcgtctcttt tggtgattgc tggacttgtg agtggtcagg caatcgccgc
    12841 gcctgaatcc cccccgcatg ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg
    12901 gcaagtcaac acctttaacc catccaaagc gagcagtggg ttaattgtcg atacccttgc
    12961 cgcccagttt tatgatcgac tgctggatgt cgatccctat acctatcgcc tgatgccgga
    13021 acttgccgaa agctgggaag tactcgacaa cggcgcgacc tatcgcttcc acctgcatcg
    13081 cgatgttccg tttcaaaaaa ccgactggtt tactcccact cgtaaaatga atgccgacga
    13141 tgtggtgttt accttccagc gaatttttga ccgcaacaac ccgtggcata acgtcaacgg
    13201 cagcaacttc ccctacttcg acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa
    13261 actggataat cataccgttg agttccgact cgcccagccg gatgcttctt ttttgtggca
    13321 cctcgcaacc cattatgctt cggtcatgtc ggcagaatat gcccggaagt tagagaaaga
    13381 agatcgccag gagcaactcg accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga
    13441 ataccgcgcc gggcaattta ttcgcctaca acgtcatgat gacttctggc gcggtaaacc
    13501 gttaatgccg caggtagtgg tggatttagg ctccggcggc accggacgtc tgtcgaaact
    13561 cctgaccggg gaatgcgacg ttctggcctg gcctgctgcc agccagctat ccattttgcg
    13621 tgacgacccg cgcttgcgtt taacgctgcg tcctgggatg aacgtcgcct atctggcatt
    13681 taacaccgcc aaaccgccgc taaataatcc cgctgtccgc catgcactgg cactggcgat
    13741 taataaccag cgcctgatgc aatccatcta ttatggtacg gctgaaacgg cggcctctat
    13801 tttaccgcgc gcctcgtggg cctatgacaa cgaggctaaa attactgaat acaatccggc
    13861 gaaatcgcgc gaacagttga aggcgttggg gctggaaaac ttaacgctga aactgtgggt
    13921 gcccacacgt tcgcaggcgt ggaaccccag tccactgaaa actgccgaac tgattcaggc
    13981 ggatatggcg caggttggcg taaaagtggt gattgtgccg gtagaaggtc gctttcagga
    14041 ggcgcggttg atggatatga gccatgatct gacgttatcc ggttgggcga cggacagtaa
    14101 cgacccggac agtttcttcc gtcctttact gagctgcgcg gcaattcatt cacagaccaa
    14161 cctcgcccac tggtgcgatc cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca
    14221 gcagctggcg gcgcgtattg aagcctatga cgaagcgcag agtattctgg cgcaggaatt
    14281 gcccattttg ccgctggcgt cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg
    14341 tctggtactt agcccgtttg gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga
    14401 tgaggtgaaa aaaccatgat tatcttcacc ttacgccgca ttttgttatt gattgtcacc
    14461 ttgttcctgc tgaccttcgt tggcttcagc ttgagctatt tcacccctca tgcgccgtta
    14521 caaggcgcgt cactgtggaa tgcctgggtg ttctggttta acggcctgat ccactgggat
    14581 tttggtgttt ccagcattaa cggtcaaccg attgccgaac agttaaaaga ggtcttcccg
    14641 gcgacgatgg aactgtgcat cctcgccttc ggttttgcgc tgattgttgg catccccgtg
    14701 gggatgattg ccgggattac gcgccataag tggcaggata acttgatcaa cgccatcgcc
    14761 ttactgggct tttcaatccc ggtgttctgg ctggcgcttc tgttgacgct gttttgttca
    14821 ctcacgctag gttggttgcc cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg
    14881 attaccggtt ttgcgttgat tgatgcctgg ctttcggact caccatggcg ggatgaaatg
    14941 atcatgagcg caatccgcca tatgatattg cccgtgatca ccctgtcggt tgcgccaaca
    15001 actgaagtga tccgcctgat gcgtatcagc actatcgaag tgtatgacca gaactatgta
    15061 aaagcggcgg cgacccgcgg tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat
    15121 aacgcgttac cgccggttat tcctcgcctg ggcttacagt tttccaccat gctgacgctg
    15181 gcgatgatca ccgaaatggt ttttagctgg ccgggtttag ggcgctggtt aattaacgcc
    15241 atccgccagc aggattacgc agccatttcc gccggagtga tggtgtgtgg ctcactggtc
    15301 attattgtta acgtgatttc tgatattttg ggtgccatgg ctaaccctct gaaacataag
    15361 gaatggtatg ccttacgata gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac
    15421 cgcctggcgc aaattttata gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg
    15481 gctggctgta ctgtgtattt ttggcggctg gtttgcgcct tacggcatcg accagcaatt
    15541 tctcggttat caattattgc cgccatcatg gtcgcgctat ggcgaagttt ctttcttcct
    15601 ggggactgac gacctcggac gcgatgtttt aagccgttta ctgagcggag ctgcgccaac
    15661 cgtgggtggc gcatttgtgg tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac
    15721 cttcgccggg gcgacacacg gcctccgctc agcggtgctt aaccatattc tggatacctt
    15781 actggcgatc ccttcgctgc tgttggcaat tatcgttgta gcgtttgccg gaccgagttt
    15841 gtcccacgct atgtttgctg tctggctggc attgctgccg cgtatggtac gttcgattta
    15901 cagcatggtg catgacgaac tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc
    15961 atcaacgctg aatattctct ggtttgctgt gatgccaaac atcaccgccg ggctggtcac
    16021 cgagatcacc cgcgcactgt cgatggcaat tctcgatatc gccgcgctgg gctttctcga
    16081 tctcggcgca caactcccct cgcctgaatg gggagcgatg ctcggtgatg cgctggaact
    16141 gatttatgtc gccccgtgga ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt
    16201 gttggttaac ctccttggcg atggcgttcg tcgtgcgatt attgcggggg tggaataatg
    16261 ccattactcg atattcgtaa cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa
    16321 gccgtcgacc gggtaagtat gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa
    16381 tccggttccg gcaagagttt gattgcgaaa gcgatttgtg gggtgaataa agataactgg
    16441 cgtgttactg ctgaccgtat gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc
    16501 gaacggcgca aactggttgg ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt
    16561 cttgaccctt cagaacgtgt gggtcgccag ttgatgcaaa acatcccagc ctggacctat
    16621 aaaggccgtt ggtggcagcg ttttggctgg cgcaaacgcc gtgccattga actgctgcac
    16681 cgcgtgggga ttaaagatca caaagatgcg atgcgcagtt ttccctatga gttgaccgaa
    16741 ggtgaatgtc agaaagtgat gatagccatt gcactggcga atcaaccgcg tctgctgatt
    16801 gctgacgaac cgaccaactc aatggagcca acaacccagg cgcaaatctt tcgcctgctg
    16861 acgcgtctca accaaaacag caataccact attttgctta tcagccatga cttacaaatg
    16921 cttagccaat gggcggataa agttaacgtg ctttactgcg gtcaaacagt ggaaaccgcg
    16981 ccgagtaagg aattggtgac gatgccacat catccttata cccaggcgct gatccgcgcg
    17041 ataccagact tcggcagcgc gatgccgcat aaaagtcgcc tcaatacgct gcccggcgct
    17101 atcccactgc tggaacagtt accgattggt tgtcgtctgg ggccacgttg cccgtatgca
    17161 caacgagaat gcattgtgac gccacgtttg acgggggcga aaaatcatct ctatgcctgt
    17221 catttcccgc tgaacatgga gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa
    17281 tttaagtaaa acgttccgct accggaccgg ctggtttcgc cgtcagaccg tagaagcggt
    17341 aaaacccttg agctttacgc tacgtgaagg ccagacactg gcgattattg gcgagaatgg
    17401 ttcgggtaaa tccacgctgg caaaaatgct ggcgggaatg atagagccta ccagcggcga
    17461 gttattgatt gacgatcatc cactgcattt tggcgattat tccttccgca gtcagcgtat
    17521 tcgcatgatt tttcaggatc cttcgacctc gttgaatccc cgtcaacgta tctcgcaaat
    17581 tctggatttt ccactgcgcc tgaacaccga cctggaaccg gaacagcgtc gcaaacagat
    17641 tattgaaacg atgcgtatgg ttgggctgct accggatcac gtcagttact atccgcatat
    17701 gttggcaccc ggacaaaaac agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa
    17761 agtcattatt gccgatgaag cactcgcttc actggatatg tcgatgcgtt cgcagttgat
    17821 taatctgatg ctggagttac aggaaaaaca gggcatttcg tatatttatg ttacccagca
    17881 tatcggaatg atgaagcaca ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt
    17941 cgagcgaggc agcaccgcgg atgtgctggc atcgccgctg catgaactca ccaaacggct
    18001 gattgccggg cactttggcg aagcattgac agcggatgcg tggcgtaaag atcgctaatt
    18061 ttctgaatcc tccttccaga ttatccacct cattctgaca agagattaaa tcttgtctga
    18121 atgaggtgat ggataaaata aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc
    18181 aggaacgaaa gctctggcga tatcttcgca gccgacgttt tggtgatttc aaatttcgcc
    18241 gtcaacatcc ggtggggagc tacattctcg attttgcttg ctgctcggcg cgcgtagtcg
    18301 ttgagctgga tggtgggcag catgatttgg cagttgccta tgataccagg cgcactagct
    18361 ggcttgagtc gcagggctgg accgtgctgc gtttctggaa taacgagatt gattgtaatg
    18421 aggaggcggt gctggagatt attctgcagg aactgaaccg ccgatcaccc tcaccctgaa
    18481 agggcgaggg ggccgaccga gccgaatagc tgttgtggtg aaaacatgga gacagtgctg
    18541 gagaatattc ggcaaggtct gaaccatccc agccatcgcc ataaaagggt taggggctgt
    18601 atgagcctgt ttgttgctgg ggtaacaata tttgcacaat atggtcccct cgcccctctg
    18661 gggagagggt tagtgtaagg ggaaaagcgc ccaccctgcc gcagcctgct ccggtcggac
    18721 ctggcaacta tagctactca cagccaggtt gattataata accatttatc tgttcgtact
    18781 gtttactaaa acgacgaatc gcctgatttt caggcacaac aagcatcaac aataaggatt
    18841 aaagctatgg gttttctttc cggtaagcgc attctggtaa ccggtgttgc cagcaaacta
    18901 tccatcgcct acggtatcgc tcaggcgatg caccgcgaag gagctgaact ggcattcacc
    18961 taccagaacg acaaactgaa aggccgcgta gaagaatttg ccgctcaatt gggttctgac
    19021 atcgttctgc agtgcgatgt tgcagaagat gccagcatcg acaccatgtt cgctgaactg
    19081 gggaaagttt ggccgaaatt tgacggtttc gtacactcta ttggttttgc acctggcgat
    19141 cagctggatg gtgactatgt taacgccgtt acccgtgaag gcttcaaaat tgcccacgac
    19201 atcagctcct acagcttcgt tgcaatggca aaagcttgcc gctccatgct gaatccgggt
    19261 tctgccctgc tgaccctttc ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt
    19321 atgggtctgg caaaagcgtc tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt
    19381 ccggaaggtg tgcgtgttaa cgccatctct gctggtccga tccgtactct ggcggcttcc
    19441 ggtatcaaag acttccgcaa aatgctggct cattgcgaag ccgttacccc gattcgccgt
    19501 accgttacta ttgaagatgt gggtaactcc gcggcattcc tgtgctccga tctctctgcc
    19561 ggtatctccg gtgaagtggt ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa
    19621 ctcgaattga aataatcatt ctgttggtaa atattgggcg gcgttctgcc gcccgttatc
    19681 tctgttatac ctttctgata tttgttatcg ccgatccgtc tttctcccct tcccgccttg
    19741 cgtcaggata acgatttcct ttacgaccaa ggagcgccca tggaacaacg ccacatcacc
    19801 ggcaaaagcc actggtatca tgaaacgcaa tccagtactg cggagtatga cgttctgcct
    19861 ctggtcccgg aagccgcaaa ggtcagcgat ccctttctgc tcgacgtgat ccttgatgaa
    19921 gaaacgctgg ctcccttcct ttcatagctg gtccctgcgc gcgttcttgc agtggaattg
    19981 ttccctgacc agcttaccgt gacccgttca cagaccttca ccgcttatga acgcttgtct
    20041 acggccctga ctgttgctca ggtttgcggc gtccagcggt tatgtaacta ctattcggcg
    20101 cgacttacgc cgctccccgg gcctgattcc tctagggaaa gcaatcatag gttggcacaa
    20161 atcacgcaat atgcccgcca actggctagc tcgccttcta ttatcgacaa ccgatcgcac
    20221 cagcatctga atgacgtcgg tcttactgcc tgggactgtg tgttcattaa ccaaatcatt
    20281 ggttttattg gctttcaggc gcggaccatt gcgacacttc aggcttatct cgggcacccg
    20341 gtacgctggt tacccggtct ggaaatacaa aactacgccg acgcgtcact gtttgctgat
    20401 gaatcaatac gctggcgaag cagctatgag gtggaaaaac tacctgaaga gtacacaaaa
    20461 agttcaacag cagaactttg ccaactggcc gaaacactct ctctccaccc tatttcactt
    20521 tcccttctcg aaaggttgtt aaacagtaca cgggttaata cacagccgga taataagctt
    20581 gcggcgttgt tatgcgcgcg gataaatggc agtcctgctt gttttgccgc ctgtatggat
    20641 tcatcaaatg aatataaaaa aatcagcccc cttctgcgca agggcgaaaa tgaaattaac
    20701 caatgggctg accgtcattc tgttgagcac gctaccgttc aggcgataca atggctgacc
    20761 cgagcacccg atcgctttag caccgcccag ttcagccctt tactcgaaca cgaacaatca
    20821 tcaacgcaga ttattaatct gctggtatgg agcgggcttt gtggctggat aaatcgctta
    20881 aaaatcgcgt tgggtgagac atattaacct tgccgcgtca gacagattcg cgtaaaactg
    20941 tcagccgctc taatggccac caaaatagac aattatgttt caggacaacc cgctgctagc
    21001 gcagcttaaa cagcaactgc attcccagac gccacgcgct gaaggggtgg taaaagccac
    21061 agaaaaaggc tttggcttcc tggaagtcga cgcgcaaaaa agttatttca ttccgccgcc
    21121 gcagatgaaa aaagtcatgc atggcgaccg aattatcgcg gtgatccaca gtgaaaaaga
    21181 acgtgaatcc gcagagccag aagaactggt tgaaccgttc ctgactcgtt tcgtgggtaa
    21241 agttcagggc aaaaatgacc gtctggccat cgttcctgat catccactct taaaagacgc
    21301 cattccttgc cgcgcagccc gtggcctgaa ccacgagttt aaagaaggcg actgggcggt
    21361 tgccgaaatg cgccgtcatc cgctgaaagg cgatcgttct ttctatgctg aattgacaca
    21421 atacatcact tttggtgacg accactttgt accgtggtgg gttacacttg cgcgccataa
    21481 tctggaaaaa gaagcaccag acggcgtcgc taccgaaatg ctcgatgaag gtctggttcg
    21541 tgaagatctg accgcgctgg attttgtcac catcgacagt gccagcacag aagatatgga
    21601 tgacgccctt ttcgctaagg cgttgccgga tgacaaactt cagctgattg tggcgattgc
    21661 cgatccaacc gcgtggattg ctgaaggcag caagctggac aaagccgcga aaattcgcgc
    21721 attcaccaac tatctgcctg gcttcaacat ccctatgctg cctcgcgagc tttctgacga
    21781 tctctgctca ctgcgcgcca atgaagtccg cccggtactg gcatgccgca tgacgctctc
    21841 cgctgatggc gccattgaag ataatatcga attctttgcc gccaccatcg aatccaaagc
    21901 aaagctggtg tatgaccagg tttctgactg gctggaaaat accggtgact ggcagcctga
    21961 aagtgaagca attgccgaac aagtccgttt gctagcgcaa atttgccaac gccgcggcga
    22021 gtggcgtcat aaccacgcac tggtgtttaa agatcgcccg gattaccgct ttattctcgg
    22081 tgaaaaaggt gaagtgctgg atatcgtcgc cgagcctcgt cgcattgcca accgtatcgt
    22141 cgaagaagcg atgattgccg ctaacatttg tgcggcccgc gtactgcgcg ataagctcgg
    22201 ttttggcatc tataacgtgc atatgggctt tgatccggcg aatgccgacg cgctggcagc
    22261 gttgctgaaa acgcacggtc tgcatgtcga tgccgaagaa gtgctcacgc tggacggttt
    22321 ctgcaaactg cgtcgtgaac tggacgcgca accaactggt ttcctcgaca gccgcattcg
    22381 tcgcttccag tcatttgctg aaattagcac tgaacccggt cctcactttg gcctcggtct
    22441 ggaagcatac gccacctgga cttcgccgat ccgtaaatat ggcgacatga tcaaccaccg
    22501 tctgctgaaa gcggttatca aaggcgaaac tgcgacgcgc ccacaggatg aaatcactgt
    22561 ccaaatggcc gagcgtcgcc gtctcaaccg gatggcagaa cgtgatgttg gtgactggtt
    22621 atacgcacgc ttcctgaaag acaaagccgg gaccgacacc cgtttcgcag cggaaattgt
    22681 cgatatcagc cgtggcggca tgcgtgtgcg tttggttgat aacggcgcta tcgcctttat
    22741 tccggcacct ttcttacacg ctatgcgcga tgaactggtt tgcagccagg aaaacggcac
    22801 tgtacaaatt aaaggtgaaa cggtttacaa agtaactgac gttattgacg tcaccattgc
    22861 cgaagtccgc atggaaaccc gcagcattat tgcgcgcccg gtcgcgtaat ctcctttcac
    22921 ggcctattcc tcatggatgg gccgtttatt tccccgctct accttcgtta tatccaggca
    22981 gattttttaa ctgactttcg tttgaaaact ggcgtttttc caaaactgga ttacaaataa
    23041 atatataaaa aaaacaatgc gtttagccgc cgttaatttc atgtaagtca
//
LOCUS       DAJTNA010000052        23074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTNA010000052 DAJTNA010000000
VERSION     DAJTNA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23074
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.053"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            128..625
                     /gene="ftnA"
                     /locus_tag="OO161_001692"
     CDS             128..625
                     /gene="ftnA"
                     /locus_tag="OO161_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5462803.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(663..902)
                     /locus_tag="OO161_001693"
     CDS             complement(663..902)
                     /locus_tag="OO161_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5462804.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1093..2304
                     /gene="tyrP"
                     /locus_tag="OO161_001694"
     CDS             1093..2304
                     /gene="tyrP"
                     /locus_tag="OO161_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5462805.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2366..3031)
                     /gene="yecA"
                     /locus_tag="OO161_001695"
     CDS             complement(2366..3031)
                     /gene="yecA"
                     /locus_tag="OO161_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5462806.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3227..3313)
                     /locus_tag="OO161_001696"
     tRNA            complement(3227..3313)
                     /locus_tag="OO161_001696"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3277..3279),aa:Leu,seq:taa)
     gene            complement(3326..3399)
                     /locus_tag="OO161_001697"
     tRNA            complement(3326..3399)
                     /locus_tag="OO161_001697"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3365..3367),aa:Cys,seq:gca)
     gene            complement(3454..3529)
                     /locus_tag="OO161_001698"
     tRNA            complement(3454..3529)
                     /locus_tag="OO161_001698"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3494..3496),aa:Gly,seq:gcc)
     gene            complement(3681..4229)
                     /gene="pgsA"
                     /locus_tag="OO161_001699"
     CDS             complement(3681..4229)
                     /gene="pgsA"
                     /locus_tag="OO161_001699"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5462807.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4286..6118)
                     /gene="uvrC"
                     /locus_tag="OO161_001700"
     CDS             complement(4286..6118)
                     /gene="uvrC"
                     /locus_tag="OO161_001700"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5462808.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLLQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6115..6771)
                     /gene="uvrY"
                     /locus_tag="OO161_001701"
     CDS             complement(6115..6771)
                     /gene="uvrY"
                     /locus_tag="OO161_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5462809.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7066..7242
                     /locus_tag="OO161_001702"
     CDS             7066..7242
                     /locus_tag="OO161_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462810.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7229..7453
                     /locus_tag="OO161_001703"
     CDS             7229..7453
                     /locus_tag="OO161_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5462811.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7520..8242)
                     /gene="sdiA"
                     /locus_tag="OO161_001704"
     CDS             complement(7520..8242)
                     /gene="sdiA"
                     /locus_tag="OO161_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR5462812.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8472..9224)
                     /gene="yecC"
                     /locus_tag="OO161_001705"
     CDS             complement(8472..9224)
                     /gene="yecC"
                     /locus_tag="OO161_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5462813.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9221..9889)
                     /gene="tcyL"
                     /locus_tag="OO161_001706"
     CDS             complement(9221..9889)
                     /gene="tcyL"
                     /locus_tag="OO161_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5462814.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9904..10890)
                     /gene="dcyD"
                     /locus_tag="OO161_001707"
     CDS             complement(9904..10890)
                     /gene="dcyD"
                     /locus_tag="OO161_001707"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5462815.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10995..11795)
                     /gene="tcyJ"
                     /locus_tag="OO161_001708"
     CDS             complement(10995..11795)
                     /gene="tcyJ"
                     /locus_tag="OO161_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5462816.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(12366..13049)
                     /gene="fliA"
                     /locus_tag="OO161_001709"
                     /pseudo
     CDS             complement(12366..13049)
                     /gene="fliA"
                     /locus_tag="OO161_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(13369..15075)
                     /locus_tag="OO161_001710"
     CDS             complement(13369..15075)
                     /locus_tag="OO161_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5462817.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNALAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSVKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGTGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLL
                     QG"
     gene            15341..16747
                     /gene="fliD"
                     /locus_tag="OO161_001711"
     CDS             15341..16747
                     /gene="fliD"
                     /locus_tag="OO161_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5462818.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKNYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            16772..17182
                     /gene="fliS"
                     /locus_tag="OO161_001712"
     CDS             16772..17182
                     /gene="fliS"
                     /locus_tag="OO161_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5462819.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            17182..17548
                     /gene="fliT"
                     /locus_tag="OO161_001713"
                     /pseudo
     CDS             17182..17548
                     /gene="fliT"
                     /locus_tag="OO161_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            17626..19113
                     /gene="amyA"
                     /locus_tag="OO161_001714"
     CDS             17626..19113
                     /gene="amyA"
                     /locus_tag="OO161_001714"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5462820.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(19147..19560)
                     /locus_tag="OO161_001715"
     CDS             complement(19147..19560)
                     /locus_tag="OO161_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5462821.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            19747..20952
                     /gene="yedE"
                     /locus_tag="OO161_001716"
     CDS             19747..20952
                     /gene="yedE"
                     /locus_tag="OO161_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5462822.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            20949..21182
                     /gene="yedF"
                     /locus_tag="OO161_001717"
     CDS             20949..21182
                     /gene="yedF"
                     /locus_tag="OO161_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5462823.1"
                     /translation="MKNIVPDYRLYMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            21289..21960
                     /locus_tag="OO161_001718"
     CDS             21289..21960
                     /locus_tag="OO161_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR5462824.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARIETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(22000..23074)
                     /locus_tag="OO161_001719"
                     /pseudo
     CDS             complement(22000..23074)
                     /locus_tag="OO161_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5625 a         5947 c         5617 g         5885 t
ORIGIN      
        1 cggtaatgcg ttttattccc attcgtattt ttaataattg aagtttatat tttacctttt
       61 gcaaataata aaataacaaa ttatagtgac gccacagcaa caaatataac ctttgtggag
      121 cactatcatg ctgaaaccag aaatgattga aaaacttaat gagcagatga acctggaact
      181 gtactcttca ctgctttatc agcaaatgag cgcctggtgc agctatcata ccttcgaagg
      241 tgctgccgcg ttcctgcgcc gtcacgccca ggaagagatg acgcatatgc agcatctgtt
      301 tgattacctg actgataccg gcaatttacc gcgtattaat accgttgaat ctccgtttgc
      361 tgaatattcc tcacttgatg aattattcca ggaaacctat aaacacgaac aattaatcac
      421 ccagaaaatt aacgaactgg ctcatgctgc aatgaccaat caggactacc caacatttaa
      481 tttcctgcaa tggtatgttt ctgagcagca tgaagaagag aaactgttca aatcgattat
      541 tgataaatta agcctggcag gcaaaagcgg cgaaggtctg tattttatcg acaaagaact
      601 ctctaccctc gacacacaaa actaatgctc tccggcggca ggtttactgc cgccgtttca
      661 aatcagtgac ggcaaatctt actctgatcg gtcgaaaaac cgtctttcga aggtataaat
      721 ttgccttttg ctgccagaaa cgctaccagc tctcccgctg tcatcccttc tgccgagcag
      781 gtgtgaaaac gtgcctgttc accaaaacgc gctttaattg ctgcttccag actggcatgc
      841 gtatattgct cgcctgattc aatcatcata tttaacactt catgaccgtg aatagagtcc
      901 atcgttcctc ctcaaaaaaa gcctagcgta gcgattgccg cttatgaaga ctttgcgcca
      961 gagcagaact gaatgctttt tattgtacat ttatatttac accatatgta acgtcggttt
     1021 gacgaagcag ccgttatgcc ttaacctgcg ccgcagatat cactcataaa gatcgtcagg
     1081 acagaagaaa gcgtgaaaaa cagaactctg ggaagtgttt ttatcgtggc gggaaccaca
     1141 attggcgcag gcatgctggc aatgccgctg gctgcggcag gtgttggttt tagcgtcacg
     1201 ttaatcttgt tgattgggct ttgggcgttg atgtgctaca cggcgctatt actgctggag
     1261 gtgtaccagc atgttccggc agataccggt ctgggcacgc tggcaaaacg ctatctggga
     1321 cgctacggtc aatggctgac gggcttcagt atgatgttct taatgtatgc tctgactgcg
     1381 gcatacatca gcggtgccgg tgaattgttg gcctccagca tcagcgactg gacaggtatt
     1441 tcaatgtcgg caaccgctgg cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt
     1501 gttggaacat cgctggtcga tttatttaac cgttttctgt tcagcgccaa aattattttt
     1561 ctggtggtga tgctggtatt gctgctaccg catattcaca aagtgaatct tttaaccctg
     1621 ccgttgcaac aggggctggc tctgtcagca atcccggtga tttttacctc atttggtttt
     1681 cacggtagcg tgccgagtat tgtcagctat atggatggca acgttcgtaa gctacgcagg
     1741 gtgtttataa ccggtagtgc gatccccctg gtagcatata ttttctggca ggtggcaacg
     1801 cttggcagca ttgattcaac aacctttatg ggattgctgg ctaatcatgc tggattaaac
     1861 gggctgttac aggcgttacg cgaaatggtg gcctctccgc atgttgagct ggcagtgcat
     1921 ttatttgctg atttagccct cgccacgtca tttctcggcg ttgcgttagg cttatttgat
     1981 tatctggctg atttatttca gcgttcaaat accgttggtg gacggttgca aactggtgca
     2041 attacctttc tgccgccgtt ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg
     2101 ctgggttacg ccggtgtggc gctggcggta ctggcattga ttatcccttc gctgttgacc
     2161 tggcaaagca gaaagcacaa tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg
     2221 ctggtggtgg tgtttctctg tggtattgct gtgattggcg tgcaattttt gattgcggca
     2281 gggttgttac cagaagtggg gtgatcagat agcctcaaat tccttattgg gtgccagaat
     2341 taacgctgac acccaatttg gcctcttaat gcaggcagca ctgcttaaat ttcttaccac
     2401 taccgcacgg gcaaggatcg ttacgccccg gtttctcttc tgctttgatc ggttgctgaa
     2461 cagctttttc ctgcggatgc gccatccagt acgcatgtag atcaagcgcc gccagtcgaa
     2521 tggcatctac gctctcttca aacgcttctg gcgacatctt ttctacccgc tcgaagtttt
     2581 cctcagtacc gtgcagcgca atcgcctcca gcgctggttt taacgaatcg ggcaacgttg
     2641 accagtcaga aagtgccacg ccccgcatat agccaaagca ccactcctca acaatcgtca
     2701 gctcgctgcc atcaacttct cgcaagccga ataacggctc aaactgctcc gggaattcgt
     2761 tcagacgctc tgcggtatcg gccatatgtt gaaaagccag gttcataaag cgcgtcatct
     2821 ctttctctga cgcccagcgc ggcacatagt cagccccacc ccacacggca accagccact
     2881 gttccggttc aatctcttgc ggagaactca acaccgccgt caataaatcg tccagctccg
     2941 ccacatcaag gatggcgtgg ttagtgttgt atttggtcag aatatcgtcc agccattcca
     3001 actcactttc gtttaacggt cccgttttca tacgcttttc cttgtggatc tcaactcgcc
     3061 agcacctatc ttacatgccg gtccgtatca gagatacttt ttgagtggct ttgccggtga
     3121 ttaaaaatta aggaggatgt aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg
     3181 tttcacgaca ctgcttattg ctttgatttt attcttatct ttcccatggt acccggagcg
     3241 ggacttgaac ccgcacagcg cgaacgccga gggattttaa atcccttgtg tctaccgatt
     3301 ccaccatccg ggctcgggaa gaaagtggag gcgcgttccg gagtcgaacc ggactagacg
     3361 gatttgcaat ccgctacata accgctttgt taacgcgcca aattcttcag gcctttcagc
     3421 cagacatccg cttgacgccg atgtctttta aactggagcg ggaaacgaga ctcgaactcg
     3481 cgaccccgac cttggcaagg tcgtgctcta ccaactgagc tattcccgca ttcattaagc
     3541 aatcagttaa tcacttgatt ttattatcgt ctggcaatca gtgccgcctt tcgatgcgtt
     3601 gcattctact tacctggcgc gatgagtcaa cgatattttt caccactttt gatcgtttgc
     3661 tgaaaattac gccgaaacga tcactgatca agcaaatctg cacgcgcagc gctcaaatat
     3721 tgcaacattg accacagagt cagtaccgca gccacaaaga aaagtgcaat accggcgtac
     3781 tcaacccaaa tgttcggacg ccacagcagc caggccaacg ccaccatctg ggcagtggtt
     3841 ttcactttcc caatccaaga gacagccacg ctactgcgtt tacccaactc cgccatccat
     3901 tcgcgtagcg cagaaataat aatttcacgg gcgatcatcg ttgccgccgg tagggtcacc
     3961 caccagctgt gataatgctc ggttaccagc accatggcga tagccacgag aactttatct
     4021 gctacagggt caaggaaagc accaaaccgg gtactctggt tccagcggcg tgccagaaaa
     4081 ccatcgaacc agtcagtcac cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac
     4141 caggtgacag gcagataaaa gaccaataca aagaatggga taaggatgac acggaatagt
     4201 gtaagcaacg tagggatatt aaattgcata atgacgggta actatctgtt gtcagtaaga
     4261 ttacccctat gttgctacag agacatcaat gtttcaacga ccagaagatc ttttctgcca
     4321 gaccttgcga aatacccggc acttttgcaa tttcctcgac gctggcgtta cgtaaacctt
     4381 gcaaaccgcc catatatttc aacaacattt gccgacgttt tggcccgacg ccttcaatgg
     4441 tttccaggga actggtattt ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt
     4501 gatcatgtga ttcatcgcga atatgctgga taacatgcag cgcgggtgaa tctggcggca
     4561 aactaaatcc ctcaccttcc ggctcaaaga acagcgtttc cagtccagcc ttacgatctg
     4621 ctcctttggc aacgccaagt agcagcggat gatttttatc ccatgagaca tccagttcgg
     4681 cgaagacatt tttcgcctgc gcaagctggc ctttgccgcc gtcgataagg atcacatccg
     4741 ggatcttact gtcgtcaatg gctttaccat aacgccgacg cagcacctga ttcatcgccg
     4801 cataatcatc gcccggcgtg atgcctgtaa tgttatagcg tcgatactcc gcacgcagcg
     4861 ggccgttagc atcaaacacc acacaggaag cgacggtttg ttcgcccatg gtatggctga
     4921 tgtcaaagca ctccatccgc ttcacttccg gcaatttcaa cacgctggca agcgcggtca
     4981 gtcgctggtg aacggtagat tgctgcaaaa gtttgctggt taaggccgtc gccgcattgg
     5041 tgcgcgcgag tttcagataa cgcgccctat cgccgcgagg tttggtttga acattaatct
     5101 tgcgtcccgc cagttctgaa agggaatcgg cgagcagcgt tttatcgcta agattaaaat
     5161 cgagcaggat ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag aactgaccta
     5221 cgaaggtttc taccacctcg ctcagttccg taccgccagg cactttcggg aaatagctgc
     5281 ggctgccgag cactttgccc tgacgaatga acaatacgtg gacacaagcc atgcccgcat
     5341 cgaacgccac accaataacg tcaaggtcgt cgccggtatt ggaaacaaac tgtttttcgg
     5401 tgacgcgtcg caccgcctga atttggtcgc gaatacgtgc cgcttcttca aactccagat
     5461 tctgactggc agtttccata cggctaatga gttgcgtaag cacctgatca tctttgccag
     5521 acaaaaacag gcgcacatac tcgacctgct gagcgtattc ttcttcactc accagtcctt
     5581 caacgcacgg tcccagacag cgccctatct gatattgcag acacggacgc gagcgattgc
     5641 gataaacact attttcgcac tggcgaatgg ggaaaatctt ttgcagtagc gccagagttt
     5701 cacgtacggc atagccattc gggaacgggc cgaaatattc acctttggca tgcttcgcac
     5761 cacgatgcat cgccagacgc gggtgggtat cgccactcag gaagataaaa ggataagatt
     5821 tatcatcgcg tagcaaaacg ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca
     5881 gcaacagcgc ttcggtttct gtatgagtaa ccgttacatc aatttgctga atctgggcga
     5941 ccagcgcttc ggttttgcgc gaagcgaggt tgctacggaa atagctggaa agccgttttt
     6001 tcaggtcttt cgctttgccg acatagataa ccgtaccacc agcatcgtac atgcgataaa
     6061 cgcctggctg gctggttacg gtttttaaaa acgcttttgc gtcaaactga tcactcactg
     6121 acttgataat gtctccgcat tacacagacc atggcgaatt gccaggtgag tcagctcaac
     6181 atcgccatga atgtttagtt tactgaacat acgatagcgg tagctgttca ccgttttcgg
     6241 actgagattg agctgttctg agatctcatt gaccttctgg cccttggtga tcatcagcat
     6301 aatctgcaat tcacgttcag acaaactggc aaatgggctt tctgtttttt ctggttcgat
     6361 ctggcttaac gccatttgtt gagcgatgtc agaagcaatg taacgctgcc ctgaatagac
     6421 agaacgaatc gcactcacga cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc
     6481 accggcctgc atgactttcg ctggtaaagg gttttctgta tgaacggtaa gcatgatgat
     6541 tttgacatca gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg
     6601 catactcatg tccattagca ccacgtcaac ggcatttgtc cggcaccact taacggcgtc
     6661 ttcaccgcac gatgcctcac cgacgacttt tatacccttt atatcttcca gaatgcgtcg
     6721 tatccctgcg cgcaccagtt cgtggtcatc aacaagtaga acgttgatca aagaaatatc
     6781 tccagaaata gggataacgc tactgatagt tagtcattcg tatattagcg gtttttattg
     6841 caactttgaa acgttaaaaa tggtgggtat tcgatttttc tctcgttttt gtcaattcga
     6901 ttgtccacag ttatggtcac gcccgtcacc aacggcttac cggatcgttt cacagtaagc
     6961 cctacgatga aatgtgacaa aaattgactt tattcagcaa aaatgaaaat cagcccgctg
     7021 aatatgtaac attaattaac ccgaacaata cgggcaaaaa cattcatgat aaaaatattt
     7081 attggtcatt atattaacgt attttatagc actgccgata tcacgctcaa aaaacaacca
     7141 ctgctatttt tagcaaagct tatggtatac tccgccgcct taacattttt caccgcaaat
     7201 tttcattgca acatgacgag gaaaataaat gagtacgcct gatttttcta ctgccgagaa
     7261 taatcaagaa ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc tgatgctgca
     7321 ggcgatggga caagctgacg cgggccgcgt gatgttaaag atggaaaaac agcttgcgct
     7381 gatcgaagac gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa ttaaacaagc
     7441 ctaccgtcag taatataaaa ccggctgaga gcgtgccttt cagccggttt ttgcatctgg
     7501 cacgcagaaa gaaaagcgat caaattaagc cagtagcggc cgcgtaacag gccacctggg
     7561 tcttatttgg tgcattgatt tttttctgca tgtttttctg atggaaattg accgtattct
     7621 cagagattga caaaatcatc gctatctctg ctgatgtctt cccttccgcc gtccatttca
     7681 gaatttcttt ttcgcgcttg ctgaaattca tctctggcgt catcactatt tcatcattta
     7741 aacgcatcag agccatcaga ctttcgcgca ccagtaactg catttttaat tgcagttcat
     7801 cactaagaat gggtatttcg cgcgtgctgc aacgggaaaa ggacaaaaag cccagcgccc
     7861 gatttggcag cattaaatac tgagtgacac cgcggcgtaa accatgtgcg tgcgcggctt
     7921 cccataatgg ctgtgcttcg ctgaataagt catcattcca cattaaatgg ccctgactaa
     7981 agttttcagg gttcagcaca ggatcaattg cgagaaaatt ttttgcctga taataactaa
     8041 cccacgactc agggtaattg gtgtagaaag ccactttagg tcgcgtgaat ggtaccgggt
     8101 ggcgaacaca taacgaatag taatcgtact ccagctgctg agcctgaagc tctatttcat
     8161 ggtagacctc ttctgcggcc tccatcctct gaaaacgcaa cagcatcgtg cgacgccagc
     8221 tgaaaaaatc cgtatcctgc atagtaaacc gcaacgcccc tgagagtgag tatcatttat
     8281 aatgataatt caaactatca cataaataag atttatatat aatttatatt attcaggcaa
     8341 tgaattactt ttgcaagcca tcgcattctc ttatgttatt aatgagttat gctgatttgt
     8401 taagcagttt tatcacgctt gaaatggcgt cccgccccga caggtgaatc gtcggggctg
     8461 attttttctt attattgcag cagaaacttc tcgaggaact gtcgggtgcg aggctgctgg
     8521 ggatcggcaa ataacgcttt tgctgccccc tgctcgacta tccgccccag gtccataaaa
     8581 atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat caccatcgtg
     8641 cgcttttcct gcgccagctg gcggattgtg ttcaggactt cgcccaccag ctctggatcc
     8701 agcgctgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag cgcacgcgca
     8761 atcgctacac gctgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg
     8821 gccagcccaa ctttcgccag cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg
     8881 cctttgacga tcaccggccc ttcaataatg ttttccagca ccgtacgatg cggaaacaaa
     8941 ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc
     9001 tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc
     9061 ggttgttcca gcagatttat gctgcgtagc aacgtggttt tgccggaacc actcggaccg
     9121 ataattgcca ccacttcgcc aggctttacc tcgaggtcga taccgtgcag caccgtctga
     9181 ccgtggaatt ttttcaccag gttcttaact tcaatggcac tcatttgggt tctctctcct
     9241 ggcgattaag ttgattctca aaatggttct gcaacgtcga taacacagtc gccatgatcc
     9301 agtagatgag cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca
     9361 actgcgcctg acggaacagc tccggcacct ggattgtcgc ggccagcgac gtatctttta
     9421 ccagactgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa
     9481 tcgcgcgacg catggtctgc cacggcgtca taccaatact ggcagcggct tcccactgac
     9541 ctttatcaat agaagaaatg gcggcgcgca gcgtttcggc ggcataggcg gcagtgttca
     9601 gcgacagacc aatcattgct gacggaatgg gatctaattc gataccaaac tgcggcaggc
     9661 cgtaatagat cataaacagt tgtgcgatga gcggcgtacc acgaaaaata gagatataaa
     9721 aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc
     9781 cgagtagtaa gccaaaaaac atgccgccga tactgagttg cagggtatac ccagcccctt
     9841 tcaacaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgttcccg
     9901 gagctaaacg tggggatgat aggcgaacag cgcaggcgcg ccgccggtat gaataaacag
     9961 aatcggccct tcatctttga agcgtttctg actgataccg tcaatcagcc ccgccatcgc
    10021 ttttccggta tacacaggat caagcagaat gccttccaga cgcgccagca atttcactgc
    10081 ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtaa
    10141 aatttccgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac
    10201 tttcggcaat tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg
    10261 catcaggtgt tccagcccaa cagccaatcc ggcgtgagtt ccggcactgc ccgatgcgac
    10321 caccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc
    10381 cacataaccc agcgcgccca gagcattaga accgccaacc ggaatgacat acgggcgaaa
    10441 gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc
    10501 gtcgcacatt tcaatctggg tattgaacag attcagcaac aaacgattgc cgttggttaa
    10561 atagttttct gcggttgtgc caataggatt ttccagcagc gccacgcagt gcaggccgag
    10621 tttcgccgca actgcggcag tctgccgcac atggttagac tggatcgcgc cggcagtaat
    10681 cagcgtatcg gcaccttcgc gcagggcatc tgccgcgaga aattccagct tacgtaattt
    10741 attgccgccc attgccatgg gtgtgacgtc atcccgtttg atgaaaattt cccgtccaag
    10801 ataatcagaa aagcgcggca gatattcgag cggcgttggc gcgccgataa actccagccg
    10861 tggaaaacgg gttaaattat gcagtggcat aatagcctcc gatgtgtgtt gttgtgattt
    10921 tcttattatg cacgctgaaa acgcgttaat aaaaaaggcg ctagtgaaag cgcccttttt
    10981 tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga aagggcttgc
    11041 agagtgccat ctttttgcat ttccgcaatt gcatcgttca ctgctttcag caggtcttca
    11101 tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc ggttactgcc
    11161 agcgtatcgt tggttttctt caccagatcc agcgccgcca gacggtcaac gaggatcgca
    11221 tcgatacggc ctacgcgcag atcctgatat ttggtcgggt catcatcata ggtacgcaca
    11281 tcgacgccct gaacattctg ccgcagccac tcttcatagt tggtacccag accgacaccc
    11341 acttttttgc ctttcagatc agcagccgtt ttaatggtgc cttcgttacc ttttttcacc
    11401 agcgcctgaa taccagaaat ggtgtacggg gtagagaaat cgtatttttt cttgcgctca
    11461 tcagaaatgg tgacctgatt aatcaccaca tcaatacgtt tagagtccag cgacgctagc
    11521 ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt tgccagctgt
    11581 tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa actgaacggc
    11641 ggataagttc cttccagccc taccagtagc gtaccgcgct ctttgacttt attaagcaga
    11701 ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgccacggc catcacaccc
    11761 atcaatgcct gacgtcccag atgtgctaat ttcatattca ccccgaatgt tgttatgtct
    11821 gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca tcttattctt
    11881 atttaatata tatcagaagc aggcaggctg gaggatttct gcacaagtcc ggtacacgtt
    11941 tgacgctgat agtgttgata cttccgtaat gcgatgcggt aattgttggt gctggtggtg
    12001 ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagctgatc gagggaaata
    12061 ttttgctcat gtagattatt ccccaaacgg cgcaaacgaa cgacatattc acgcaccgta
    12121 cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat atcgaaaaaa
    12181 tcactctgcg ttttgcaatg taaatcaccg caaaatcggc acaatgccgc ccatgactgt
    12241 tgttccactt gccagccatt ttcatcaagc agcgtgtcca ggcgggaaat ctcgatttta
    12301 ttcactattt tgccgtcgcg aactaaggta atgcgatcga gtaatttacg gcaatgcgcg
    12361 caatgggcct ggctgtgtaa ctgactgacc cgcgattccc cgacctccag caccgcgcca
    12421 atctctttga gattcagctc ttcctggtaa tagagggtta ataccagttt ttcgcgctcc
    12481 ggcaacgttt cgatagcttc catcacccgc tggcgcagat tactgtccag cagttgttgt
    12541 agcgggtttt ctcgctgatg atcatcagta accagttcga tgctatcgcc gtgctcttcg
    12601 cgccactcat cgtaggagaa aagctggctg ttattggtgt cgagcaacat ttggcgataa
    12661 tcggcaatat cgatccctaa acgttccgct acctcagttt ccgtggcgtt gcggccaagt
    12721 tcctgctcca gttgccctat tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc
    12781 ggcacccagt cacggctgcg aagttcatcc agcatagcgc cacggatacg ctgcactgcg
    12841 taagttgtaa atgccgttcc ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca
    12901 atgccgcccg cctgtagcag atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg
    12961 cgcaatgctt cgtgacgcac cagcgggaca taacgctgcc acagagagtg tttatccatt
    13021 acaccttcag cggtatagag tgaattcacg ataaacagcc ctgcgttata tgagttatcg
    13081 gcatgattat ccgtttctgc agggttttta atcggacgat tagtgggtga aatgaggggt
    13141 tatttggggg ttaccggtaa attccatgca gaaaaaaccc cgccggtggc ggggaagcac
    13201 gttgctgaca aattgcgctt tatgttgccg gatgcggcgt aaacgcctta tccggcctac
    13261 aaaaatgtgc aaattcaata aattgcaatt caccttgtag gcctgataag tgcagcgcat
    13321 caggcaattt ggcgttgccg tcagtctcag ttaatcaggt tacgacgatt aaccctgcag
    13381 cagagacaga acctgctgcg gtacctggtt ggcttttgcc agcacggagt taccggcctg
    13441 ctggatgatc tgcgctttcg acatgttgga cacttcggtc gcatagtcgg cgtcctgaat
    13501 acgggactgc gcttcagaca ggttggtagt ggtgttgttc aggttggtga ctgcagaatc
    13561 cagacggttc tgaatagcac ccagggatga acggaatttg tcgatggagc tgatagcttc
    13621 atccagggca gccagaggat ctttggttgc agtgcctgca ctggtggttt cagtggtcag
    13681 tttgcctgta gagttaacat aagcctgtgc cccaacagta gctgcaaatt taccagttgc
    13741 acctgtgcca tttgaagcta cagttactgt accggtatcc ttatcgacgt tgtagttgat
    13801 agtgtaagat gcggttgtgg tgagctcttt gtctgcatca gtataggtgt cattcgaatt
    13861 accagcacca ttagtattcg caccaccgaa ggacgccgtt gcagactgaa tacctgtatt
    13921 atacttaatt gtcgcaccag taactgctgt gttagccgtt gcagcactct gcactgtgct
    13981 cagtacagta tcagcgctta cagttttggt gtaggttacg ccagaagttg ccgctgcacc
    14041 aaagttataa gttgtgccag acaactggat agaaccagtt gtcgctaagg tactaccgct
    14101 agcaaacaga tcggacaatt tcactgaaga tgcaatgccg gcgttgtttg tagataggtt
    14161 accagtggcg tccaggtacg cggctttacc accgatggtc acatcgccat tagcatctac
    14221 atcaaacttc acatcaccag tactacgagt ataaacacct gatgcagtag tgccagccgc
    14281 tggtttcaga gtattcgcaa agttaacaac atcgccatct gcaactgttg cttgagtggt
    14341 gaagttccct ttagccgcat cataagtata gatcgcagca ctcgagccag tagtagtaac
    14401 tgtatctccg gttttcaggc gagacagtgc atcgctagcg ctgagtgctg tattgtttgt
    14461 ggtcacagca taaggacctg ttcccgttgc accagcagcg gtcagatcgc tgactgtagc
    14521 agctttgttc gcaatagtgc ctttgccatt cacgttaaaa ccattcagcc ccagcgtatc
    14581 tgagtcaatt ttcttcagat caatcgtgat agtctggccg tcattcgcac caacctgaat
    14641 tttcattgaa ccgtctttcg ccagtgcgtt cacgccgttg aactgggtct ggccagatac
    14701 gcggtcaatt tcgtccagac gggatttgat ttcgtcctga atggagtcca gatccgaatc
    14761 ggagttagtc ccggtagaag cctgaaccgt cagttcacga atacgctgta agttgttgtt
    14821 gatttcggac agcgcgcctt cagtggtctg cgcaacggag ataccgtcgt tggcgttacg
    14881 ggccgcctga gtcaggcctt taatattaga agtaaaacgg ttagcaatcg cctgacctgc
    14941 ggcgtcatcc ttcgcgctgt taatacgcaa gccagaagac agacgctcga tagaactcga
    15001 cagcgcagac tggttcttgt tgatattatt ttgagtgatc agcgagaggc tgttggtatt
    15061 aatgacttgt gccatgattc gttatcctat attgcaagtc gttgattacg tattgggttt
    15121 ccacccgtcg gctcaatcgc cgtcaaccct gttatcgtct gtcgtaaaac aacctttaga
    15181 atttttttca caaacagcca ttttttgtta gtcgccgaaa tactcttttc tctgcccctt
    15241 attcccgcta ttaaaaaaaa caattaaacg taaactttgc gcaattcagg ccgataaccc
    15301 cggtattcgt tttacgtgtc gaaagataaa aggaaatcgc atggcaagta tttcatcgct
    15361 gggagtcggg tcaggtctgg atttaagttc aatccttgat agcctcaccg ccgcgcaaaa
    15421 agcgacgcta acccctattt caaatcagca atcgtcgttt accgctaaac ttagcgccta
    15481 cggtacgctg aaaagcgcgc tgacgacttt ccagaccgcc aatactgcat tgtctaaagc
    15541 cgatcttttt tccgccacca gcactaccag cagcaccacc gcgttcagtg ccaccactgc
    15601 gggtaacgcc atcgccggga aatacaccat cagcgtcacc catctggcgc aggcgcaaac
    15661 cctgaccacg cgcaccacca gagatgatac gaaaacggcg atcgccacca gcgacagcaa
    15721 actcaccatt caacaaggcg gcgacaaaga tccgattacc attgatatca gcgcggctaa
    15781 ctcatcgtta agcgggatcc gtgatgctat caacaacgca aaagcgggcg taagtgcgag
    15841 catcattaac gtgggtaacg gtgaatatcg tctgtcagtc acatcaaatg acaccggcct
    15901 tgataatgcg atgacactct cggtcagcgg tgatgatgcg ctacaaagtt ttatgggcta
    15961 tgacgccagt gccagcagca acggtatgga ggtctcggtc gccgcccaga atgcgcagct
    16021 gacggtcaac aacgtcgcca ttgagaacag cagcaacacc atcagcgacg cgctggaaaa
    16081 catcaccctg aacctgaacg atgtcaccac gggcaaccag acgctaacca tcactcagga
    16141 cacctccaaa gcgcaaacgg cgattaaaga ctgggtgaat gcctataact cgttaataga
    16201 taccttcagt agcctgacca aatacaccgc cgtagatgcg ggagctgata gccagagttc
    16261 tagcaatggc gcactgctcg gcgactccac gctgcggacg attcagacgc agttgaagtc
    16321 gatgctgagt aataccgtca gttcttccag ctataaaacg ctggcgcaga ttggtatcac
    16381 gaccgatccc agcgatggca aactggaact ggatgccgac aaactcaccg ctgcactgaa
    16441 aaaagatgcc agcggcgtag gtgcattgat tgttggcgat ggtaaaaaaa ccggcatcac
    16501 gaccaccatc ggcagcaacc tgaccagttg gctttcgaca acgggcatta ttaaagccgc
    16561 taccgatggc gttagtaaga ccctgaataa gttaactaaa aactacaacg ccgccagcga
    16621 tcgcattgat gcgcaggtcg cgcgctacaa aaaacaattt acccaactgg acgttttaat
    16681 gacctcgtta aacagcacca gcagctactt aacgcagcag ttcgaaaaca acagtaattc
    16741 caagtaatca ttattcatcg ggagacaggt catgtacgcg gcaaaaggca cccaggccta
    16801 tgcacaaatt ggcgtcgaaa gcgccgtaat gagcgccagc cagcagcagc tggtcaccat
    16861 gctatttgat ggagtgctga gcgcactggt tagagcgcgc ctgtttatgc aggacaacaa
    16921 tcagcaaggc aaaggcgtct ctttgtcaaa agcgatcaac atcattgaga acggactgcg
    16981 ggtgagtctt gatgaagaga gcaaagacga actaacccaa aacttgattg ctctttatag
    17041 ctatatggtc aggcgcttgc tgcaagccaa tttacgcaac gatgtctccg cagtcgaaga
    17101 agtggaagca ttaatgcgca atattgccga tgcctggaaa gagtcgttac tctccccttc
    17161 tttgattcag gacccagtct gatgaaccat gcaccgcatt tgtattttgc ctggcaacaa
    17221 ctcgtcgaaa aaagccagct catgttacgc ctggcaacgg aagaacaatg ggacgaactc
    17281 atcgccagcg aaatggcgta tgtgaatgcg gtgcaggagt atagcacacc tgactgaaga
    17341 ggttgccccg tccaccacga tgcaggagca gctccgcccg atgctgcgcc tgattctcga
    17401 caacgaaagc aaggtaaagc agttattaca gattcgtatg gatgaactgg cgaaactggt
    17461 cggtcagtca tcggtgcaaa aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt
    17521 gctggctccg caagataact tctcgtgaat cttaacgagc taatggttcg cgaataatcc
    17581 gattacggct acgcttctaa tgttcccctt gaatggagtc gaagaatgcg taatcccacg
    17641 ctgttacaat gttttcactg gtattacccg gaaggcggta agctctggcc tgaactggcc
    17701 gagcgcgccg acggttttaa tgatattggt atcaatatgg tctggttgcc gcccgcctat
    17761 aaaggcgcat cgggcgggta ttcggtcggc tacgactcct atgatttatt tgatttaggc
    17821 gagtttgatc agaaaggcag catccctact aaatatggcg ataaagtaca attgctggcc
    17881 gccattgacg ccctgaaacg taatgacatt gcggtgctgt tggacgtggt agtcaaccac
    17941 aaaatgggcg cggatgaaaa agaagctatt cgcgtgcagc gtgtaaatgc tgacgaccgt
    18001 acgcaaattg atgaagaaat cattgagtgc gaaggctgga cgcgttacac cttccccgcc
    18061 cgtgccgggc aatactcaca gtttatctgg gatttcaaat gttttagcgg tatcgaccat
    18121 atcgaaaacc ctgatgaaga tggcatattt aaaattgtta acgactacac cggcgaaggc
    18181 tggaacgatc aggttgatga tgaattaggt aatttcgatt atctgatggg cgagaatatc
    18241 gattttcgca atcatgccgt gacggaagag attaaatact gggcgcgctg ggtgatggaa
    18301 caaacgcaat gcgacggttt tcgtcttgat gcggtcaaac atattccggc gtggttttat
    18361 aaagagtgga tcgaacacgt acaggaagtt gcgccaaagc cgctgtttat tgtggcggag
    18421 tactggtcgc atgaagtcga taaactgcaa acgtatattg atcaggtgga aggcaaaacc
    18481 atgctgttcg atgcgccgct gcagatgaaa ttccatgaag catcgcgcat ggggcgcgac
    18541 tacgacatga cgcagatttt cacgggtaca ttagtggaag ccgatccttt ccacgccgtg
    18601 acgctcgttg ccaatcacga cacccaaccg ttgcaagccc tcgaagcgcc ggtcgaaccg
    18661 tggtttaaac cgctggcgta tgccttaatt ttgttgcggg aaaatggcgt tccttcggta
    18721 ttctatccgg acctctacgg tgcgcattac gaagatgtcg gtggtgacgg gcaaacctat
    18781 ccgatagata tgccaataat cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc
    18841 gcccacggtg tacagacgtt atttttcgac catccgaact gcattgcctt tagccgcagt
    18901 ggcaccgacg aatatcccgg ctgcgtggtg gtcatgtcga acggggatga tggcgaaaaa
    18961 accattcatc tgggagagaa ttacggcaat aaaacctggc gtgattttct ggggaaccgg
    19021 caagagagcg taataaccga cgaacacggc gaagcaacct tcttttgcaa cggcggcagc
    19081 gtcagcgtgt gggttatcga agaggtgatt taaattcatc cccggcggca agccggggag
    19141 atttcattac ggcagtggcg tcggtaacgg cgctttatcc agcgcggcgg cacactctgc
    19201 cgtcgggcga tccacacgct ccatcgtcat accatcgtac tcaatggtgt taccgtcacg
    19261 ctcaacctcg tacagctcac gttttacggt aacgttagtg agatcatcag acatcaatgt
    19321 caacttaccc ggcacagcaa tcacccgctg ccactgacgg caatccagag tatcaccctc
    19381 tttagtgaca atcaggctgc ctatcgcttc cggactgacc agcgcacgtt gcggcccttt
    19441 ggtctgccag tagcccgcca gccagtccgg cgcgggggtt ttcacgacat tgttatagtt
    19501 ttcgacttcg gcacagcccg ccagcaacat gagcgcgcct gcaattgcga gttttttcat
    19561 cattcgtcct gcatgagaag aaaagaggat tgtggcatta aagccctgat gtcgccagcc
    19621 tttccaggtt cagagagaaa tttgatctaa ctattatttt tgtgaattat taccccgaaa
    19681 ataaatttgc atgatgtatg attcgcgact cttgtttcaa accttctgag ttcagaggct
    19741 acattcatgt catggcagca attcaaacac gcctggttga ttaaattctg ggcacccatc
    19801 cctgcggtca tcgcggcggg tattctctcc acttactatt ttggcattac tggcaccttt
    19861 tgggctgtca cgggtgaatt tacccgttgg ggcggccagc tcctgcagct gtttggcgtc
    19921 catgctgaag agtggggtta ttttaaaatt atccatctgg aaggatcgcc attaacccgc
    19981 atcgacggga tgatgatcct cggcatgttt ggcggctgct ttgccgcagc gctgtgggcc
    20041 aacaatgtca aactgcgcat gccacgcagc cgtatccgca ttatgcaggc cattattggc
    20101 ggtattatcg ctggttttgg cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt
    20161 accggtattc ctcagttctc gctgcatgcc tggttctttg ccatcgccac tgccattggt
    20221 tcatggtttg gcgcacgctt taccctgctg cccatcttcc gtattcccgt gaaaatgcag
    20281 aaagtttctg ccgcctcacc gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt
    20341 ctcgggatgc tggtcttttt cggtatgctg ggctgggcgc tgctcacggc gatgaaccaa
    20401 ccaaaactgg ggctggcaat gctgtttggc gtcggctttg gtttactgat tgaacgtgcg
    20461 caaatctgct ttacttcggc gttccgcgat atgtggatca ccggacgtac ccatatggcg
    20521 aaagcaatca ttatcggtat ggcggtgagt gccatcggga tcttcagtta cgtacagtta
    20581 ggcgttgaac ccaaaatcat gtgggcggga ccaaacgcgg taattggtgg tttactgttt
    20641 ggttttggca tcgtgctggc aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa
    20701 ggccaggtgc actactggtg ggtcggtctg ggcaatgtga tcggctcgac gattctggcg
    20761 tattactggg atgatttcgc tccggcgctg gccaccgact gggacaaaat caacctgctg
    20821 aaaacctttg gcccgatggg tggcctgctg gtgacatatt tgctgttgtt tactgcgctg
    20881 atgttgatta tcggctggga aaaacgcttc ttccgccgtg cggcaccgca gactgctaag
    20941 gagatcgcat gaaaaatatc gttcctgatt accgccttta tatggtgggt gaaccctgcc
    21001 cttatccggc agtcgcaacc cttgaggcga tgccgcagtt aaaaaaaggg gaaatcctgg
    21061 aagtggtgag cgactgtcca cagtcgatca ataatattcc actggatgcg cgtaatcacg
    21121 gctatacggt gcgggatatt cagcaagacg ggccgaccat tcgttattta attcagaagt
    21181 aatccctcat tcccgctgtg gcccacagcg ggaacatcgt tgcctctgac acatagattt
    21241 tacccgcccc ctctcctatt cttttcagta tgatctcagg aggtttctat gtgtggacgc
    21301 tttgcccaat cccaaacgcg tgaagattac cttgcgcttc tcgcggaaga tattgaacgc
    21361 gatattccct acgatcccga acccattggc agatacaacg tcgcgccggg aaccaaagtt
    21421 cttctgctca gtgaacgtga tgaacacctt catctggatc cggttttctg gggatatgct
    21481 cccggatggt gggataaacc gccgctgatt aacgcccgca tagaaactgc cgcaaccagt
    21541 cgcatgttta aaccgctctg gcaacatggt cgggcaatct gttttgccga tggctggttt
    21601 gagtggaaaa aagaaggcga caaaaaacag ccttttttta tctatcgtgc tgatggacaa
    21661 cctattttta tggccgcgat aggtagcaca ccatttgagc atggtgacga agccgaagga
    21721 tttttaattg tcactgctgc agcagaccag ggtctggtag acattcatga ccgccgcccg
    21781 ctggttctgt cgccagaagc tgcacaggaa tggatgcggc aagagattgg cggtaaagaa
    21841 gcatcagaaa ttgctaccaa cggctgtgtg ccagcaaacc agttcacctg gcacccggta
    21901 tcgcgcgcgg taggtaatgt caaaaaccag ggcgcggagt taattcaacc tgtttgttga
    21961 catcctccac gccctgaagg acgaggctcc ctgctacgtt caggctgtcg cctgaatcat
    22021 ttcggtgggt tcctgcttca acgggcggcc tgactgcacc atctctccac aggcaagaac
    22081 ggcgtgcccc gccgctaaaa tgttacgagc gccatttaca tcggcgttcg ctgtatatcc
    22141 acatacctgg catctgaatt tactttgtga caggcggttt tctttcgctg tatgaccaca
    22201 gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca agcacctgac cgccacgcca
    22261 gagctgcttg tactcaagct ggcggcgcat ttcataccag ccctgatcca gtatcgaacg
    22321 gtttaaacct gattttgccc ggacattgcg ccccggctgg ctgaccgtac ccgctgctga
    22381 ctttgacatg ttgctgacct tcaaatcctc aatgactatc attgcgtggt ttttgctgac
    22441 ggtcgttgtg actttgtgaa ggtagtccct gcggatattt gcgatacagg aatgcagtcg
    22501 ctgtattttg cgtttctgct tctgccagtt gttgctgaat ttgaccttgc ggcttaactg
    22561 tcgctgaagt ctcgccagct tcttctggtt tttctgaaaa ctgtttacag gctcaaaggc
    22621 tgtgccatct gacagcgtgg cgagtttagc cacgccagca tccagcccga ccattgatgc
    22681 tgaagggtga accggagttg atacttcact ttctgtctga atactgatgt accacttacc
    22741 gcaggactgg ctgacagtga catttttcac aacacccgtg acctgacggc tgttccggta
    22801 gcgcatccag cccagtttcg gcagaaaaat acggctgttt tcctgatcga gcttaacgcc
    22861 ctgcgggtag cggaatacat cattctgtcc ccgctttttg aatcggggaa aagcagcccg
    22921 attctggaag aagtttttgt aagcccgctc aaggtctttc agtgactgtt gcaatggctg
    22981 tgacggggca tctttaagcc attacgtttc agtggcattt ttccactcaa ccagccagga
    23041 agccattttc ccgtaaggga tgtatttatt accg
//
LOCUS       DAJTNA010000053        22921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTNA010000053 DAJTNA010000000
VERSION     DAJTNA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22921
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.054"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..1083
                     /locus_tag="OO161_001720"
                     /pseudo
     CDS             1..1083
                     /locus_tag="OO161_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            1082..1318
                     /locus_tag="OO161_001721"
                     /pseudo
     CDS             1082..1318
                     /locus_tag="OO161_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="binds to the twin-arginine signal peptides of
                     certain proteins, including dimethylsulfoxide reductase
                     and trimethylamine N-oxide reductase that are translocated
                     to the cytoplasmic membrane; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
     gene            1477..2769
                     /gene="clcB"
                     /locus_tag="OO161_001722"
     CDS             1477..2769
                     /gene="clcB"
                     /locus_tag="OO161_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5462825.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLATGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDCAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTCVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(2722..3417)
                     /gene="bioD"
                     /locus_tag="OO161_001723"
     CDS             complement(2722..3417)
                     /gene="bioD"
                     /locus_tag="OO161_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5462826.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3542..4762)
                     /gene="mlc"
                     /locus_tag="OO161_001724"
                     /gene_synonym="dgsA"
     CDS             complement(3542..4762)
                     /gene="mlc"
                     /locus_tag="OO161_001724"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5462827.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGIPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPTYSQHISVESTQFSNQGTIAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(4897..5790)
                     /locus_tag="OO161_001725"
     CDS             complement(4897..5790)
                     /locus_tag="OO161_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5462828.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLAH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            5897..7149
                     /locus_tag="OO161_001726"
                     /pseudo
     CDS             5897..7149
                     /locus_tag="OO161_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            7545..7859
                     /locus_tag="OO161_001727"
     CDS             7545..7859
                     /locus_tag="OO161_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5462829.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTTKAAPAKT
                     THHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQP
                     AA"
     gene            7952..8035
                     /locus_tag="OO161_001728"
     CDS             7952..8035
                     /locus_tag="OO161_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462830.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            8135..8956
                     /locus_tag="OO161_001729"
     CDS             8135..8956
                     /locus_tag="OO161_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5462831.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGCVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(8995..9324)
                     /gene="mdtI"
                     /locus_tag="OO161_001730"
     CDS             complement(8995..9324)
                     /gene="mdtI"
                     /locus_tag="OO161_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5462832.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(9311..9676)
                     /gene="mdtJ"
                     /locus_tag="OO161_001731"
     CDS             complement(9311..9676)
                     /gene="mdtJ"
                     /locus_tag="OO161_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5462833.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            10088..11121
                     /gene="tqsA"
                     /locus_tag="OO161_001732"
                     /pseudo
     CDS             10088..11121
                     /gene="tqsA"
                     /locus_tag="OO161_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(11146..12534)
                     /gene="pntB"
                     /locus_tag="OO161_001733"
     CDS             complement(11146..12534)
                     /gene="pntB"
                     /locus_tag="OO161_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5462834.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(12545..14077)
                     /gene="pntA"
                     /locus_tag="OO161_001734"
     CDS             complement(12545..14077)
                     /gene="pntA"
                     /locus_tag="OO161_001734"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5462835.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVTRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            14601..15545
                     /locus_tag="OO161_001735"
     CDS             14601..15545
                     /locus_tag="OO161_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5462836.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            15731..17113
                     /locus_tag="OO161_001736"
     CDS             15731..17113
                     /locus_tag="OO161_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5462837.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            17150..17872
                     /gene="folM"
                     /locus_tag="OO161_001737"
     CDS             17150..17872
                     /gene="folM"
                     /locus_tag="OO161_001737"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5462838.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTTDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17869..18204)
                     /locus_tag="OO161_001738"
     CDS             complement(17869..18204)
                     /locus_tag="OO161_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5462839.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            18333..19052
                     /gene="rstA"
                     /locus_tag="OO161_001739"
     CDS             18333..19052
                     /gene="rstA"
                     /locus_tag="OO161_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5462840.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKARHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            19056..20357
                     /gene="rstB"
                     /locus_tag="OO161_001740"
     CDS             19056..20357
                     /gene="rstB"
                     /locus_tag="OO161_001740"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5462841.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            20433..21362
                     /gene="tus"
                     /locus_tag="OO161_001741"
     CDS             20433..21362
                     /gene="tus"
                     /locus_tag="OO161_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5462842.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(21359..22762)
                     /gene="fumC"
                     /locus_tag="OO161_001742"
     CDS             complement(21359..22762)
                     /gene="fumC"
                     /locus_tag="OO161_001742"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5462843.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         5547 a         5935 c         5601 g         5838 t
ORIGIN      
        1 atgacgacaa tccgcgacgg cgtccgcggc aaagataaac tggatgtgcc cattaagttc
       61 atctggaact acgcgggaaa taccctcgtt aatcagcatt ctgacatcaa caaaacgcat
      121 gaaattctgc aggacgaatc gaaatgcgaa atgattgtgg tcatcgaaaa ctttatgacc
      181 tcatcggcaa aatatgccga cattctgctg ccagacctga tgaccgttga gcaggaagat
      241 attattccta acgactacgc cggtaacatg ggatatctca ttttcctcca gcctgtcacc
      301 agcgaaaaat tcgaacgcaa accgatttac tggatcctga gtgaagtcgc gaaacgtctt
      361 ggacctgacg tctatcaaaa gttcacagaa ggtcgcacgc aggaacaatg gttacaacat
      421 ctgtacgcca aaatgcttgc caaagatccg gcgttaccgt cttacgacga actgaaaaaa
      481 atgggtatct ataagcgtaa agatcccaat ggccattttg tcgcctacaa agcatttcgt
      541 gacgaccgcg aggcaaatcc acttaaaacg ccttccggta agattgaaat ttattccagc
      601 aggctggcgg aaattgcccg tacctgggaa ctggaaaaag atgaagtgat aagcccattg
      661 ccagtttatg cctcaacctt tgaaggctgg aactcccctg aacgtagaac cttcccactg
      721 caactgtttg gtttccatta caaatcccgt actcactcga cctacggcaa tattgatctc
      781 ctgaaggctg cctgccgtca ggaggtgtgg atcaacccta tagatgcgca gaaacgtggg
      841 attgccaacg gcgatatggt gcgggtgttt aaccatcgtg gcgaagttcg gctaccagcc
      901 aaagtaacac cacgtattct ccctggagtt agcgctatgg gccagggagc ctggcacgag
      961 gccaatatgt ctggcgataa aatagaccat ggcggctgtg tgaatacgct aaccactctg
     1021 cgcccttcac cactggcgaa gggaaaaccg cagcacacta atctggtcga gatcgaaaaa
     1081 atattttggt tcactgctgt tgatggctgc gtggttggcg gagaatggtc gccagacaga
     1141 atgcgaagaa ttgctggcat ggcacctttt tccgtggtca acacgttttc ttgatgtttt
     1201 tatcgaaaaa gcagaacacc ctttctaccg tgcactgggt gaactgtctc gcttaacgct
     1261 ggcgcaatgg caatcacaac tgttaattcc tgtcgcggtt aaaccgctgt ttcgatagga
     1321 tcaccgtaat attgccggat ggtgacgatt acgcgcaatc cggcaataat aggttacagt
     1381 gtcacgtttt tttatctctt aaagcacgca ctgcttttgc ggctggcctc ttttgccgca
     1441 aaatagtcgc ccgtgtttca ttgcccattt ctgctcatgc atcatctaca catctatccg
     1501 gatctgcgca ctatgttccg ccgtctgctt atcgcaacag tcgtcggtat tctcgcggcc
     1561 tttgccgttg ccgggtttcg tcatgcgatg ctgctactgg agtggttgtt cctcaataat
     1621 gactccggca gtctggtcaa tgcagcgaca aacctttccc cctggcgacg gttgctaact
     1681 ccggcgctcg gcggactggc gacgggtttg ttgctgatgg gctggcagaa atttacccaa
     1741 caacgccctc atgcgccgac cgattacatg gaagcgttgc aaaccgatgg acagttcgat
     1801 tgcgcagcaa gcctggttaa atcgcttgcc tcgctgctgg tagtaaccag cggcagtgca
     1861 attggtcgcg aaggtgcgat gattctttta gctgcccttg ccgcctcctg ttttgcccaa
     1921 cgttttacgc cacgccagga gtggaaatta tggatcgcct gtggggccgc ggcgggaatg
     1981 gctgcggcct atcgtgcccc gcttgctggc agtttattta tagccgaagt gctgtttggc
     2041 actatgatgt tggcctctct cggcccggtg attatttccg ccgtcgtggc attgctggtt
     2101 agcaatctga ttaatcatag cgacgcgtta ctctacaacg tacaactctc agtgacggtt
     2161 caggctcgtg actatgcgct gattatcagt acatgtgtgc tggcaggtct gtgcggacca
     2221 ctgttgttaa cgttaatgaa cgcctgtcat cgtggatttg tgagtctcaa acttgcgccg
     2281 ccctggcaac tggcactagg cgggttgatc gtgggtctgc tttccctgtt cacacctgca
     2341 gtgtggggca acggctatag caccgtacaa tcctttttaa ccgccccacc actgttaatg
     2401 atcattgccg ggatcttcct ttgtaaactg tgtgccgtgc tggcgagtag cggttccggc
     2461 gcacccggtg gtgtctttac accgacgcta tttatcggtc ttgccattgg catgttgtat
     2521 ggtcgtagcc tgggattatg gttccctgat ggcgaagaaa ttacgctttt actcggattg
     2581 accgagatgg cgacactgtt ggcggcaacc acgcacgcgc cgattatgtc gacgttgatg
     2641 atatgtgaaa tgaccgggga gtatcagcta ctccccggtt tattgattgc ctgcgtcatt
     2701 gcgtcggtaa tttcgcggac gttacaccgt gactctatct accgccagca cactgcgcag
     2761 catagctaag cggatgtatt gccccagttc tcgctgttcg gcgcgcggca gataaggcag
     2821 ttcaccaatg agcggtgccg gaagtttttt acccagcaca tcaatgattt ccgcataatg
     2881 cgccagtcct gggttgattc ggttagccac ccagccaatg agcggcagcc cgtcgttggc
     2941 aatcgcctga gctgttagca gtgcatggtt aatgcaacct tcctgaatac cgacaaccat
     3001 caacaccggc aactgttcct gcaccaccca ttcagagagt ggacgcaaat cattcatcag
     3061 actgcgccag ccgccagtcc cttctaccac gacatgatcg actttatcgg tcaggtttgc
     3121 caggccgttt gaaatgaggg tgtaattgat tgggcaactg tgcgccacgc tactttcttc
     3181 ttcgcttaac gcgataggat taactgcttc ataaggcagt tcgatggttg aaacactctg
     3241 caacaccagg gcatctttat tacgcagccc ttcgggtgtc tctttgctcc ccttcgctac
     3301 gggtttatat cccgcaactg tttttccctg ggaggctaac gcttgtagca atgcgcggga
     3361 aaccaccgtt ttccctacag aagtgtctgt accggtaata aagaaacgct tcagcatcac
     3421 taactccacc gttatgcttc acaaatataa accaggaaaa taattaacct tgaaagtcta
     3481 agttatgctt tcctggccca aattgagata gcgcaaattt tggtataaca gttaaaaaat
     3541 gttaaccctg caacagacga atcaacaaag aaccgttata catcgcgtct tttaccagcg
     3601 cagcgcctgc tatcgtgccc tggttagaaa actgagtact ctcaacgcta atgtgctgac
     3661 tatacgtagg aagggcctgc tgacgtatgc tgtccgagat gacagggaag aggatatctg
     3721 ccgctttact taacggtgag ccaatcagta ttttttgtgg gttaaataaa ttcaccatga
     3781 tggcaagaat gcgcccgaca tgcgcgccca ccccggtaat gatgtctttt gccagtagat
     3841 cgccgcgcaa tgccgcctga cacaatgagt ccacggttaa cggttgccca tgtaacatcg
     3901 agctcatgga ttgattgaga cgcagctgtg ccagctcaag aatactgtcc acactggcga
     3961 tggtttcgag gcagccgtgg ttcccgcaat aacagcgttt accatatggg tcgacctgtg
     4021 tgtggcctat ttccacgaga ctactgctgc ctgcgtgtag cagatgacca tcggtaatga
     4081 cgcccgcccc cacgttgtga tcgataacca cctgaatcac atcgcgcgcc ccgcgtgagg
     4141 caccaaacaa ggcctctgcc atcgtccatg cgctgatatc atgctgaata taaaccggta
     4201 tgccggtatg ctgctccagc gcctcgccga gcggcatctc ttttacatcc tcgtagaacg
     4261 gcatgcgatg tacaatacca ttttccgtat caataattcc cggcagggtt atggcaatcg
     4321 aagttagacg ctcaagtttt ttctggtggc ggataaaaaa ctgatcgata tgggaaatga
     4381 tacgatccag caatggcaag tcatctttta acgccagttc ctgcgactct tccaccacca
     4441 gtttgctgct cagatcgcgc agagcaagga aaatctcccc gcgactaatg cgcagagaaa
     4501 gatagtgcca ggcttcagtt tcaaccacca gccccaccgc cggacggcca cggttccccg
     4561 cttctttgat ttccagctct tgcaccaagt gtgcttcgag catctcacgg acaattttag
     4621 tgatactggc aggagccagc tgcgccagac gggaaagatc gatacgcgag actggaccaa
     4681 gctgatcaat caggcgataa accgcgcccg cgttggtctg ctttatttga tcaatgtgcc
     4741 caggctggtt ttcagcaacc accgcatact ccctatattt tcgcgctccg aaataatctg
     4801 taggctatgg tgaagcactt caatacgtgt cgtcaaattt ttacttagac atgtgattaa
     4861 cagcacattt ttcgggcttt ttcgctgaaa tttccctcac ctgagagcat tcagcagatg
     4921 aatacgaaag ttacgcgaag ctggactttg ttcatgatgt ttcggccaga ccaaccacat
     4981 ttcagaaacc gcatcctctt cagcaatcgg cacccagcgc atttcgttga gctgaacacg
     5041 tttaaatgaa gcaggcaaaa ttgaaacacc cagaccggcg gaaaccagac cgatgatggt
     5101 cattgcctcg cccacctcct gagtgatgac gggcgtcaaa tggtaacgtc gcatcagccc
     5161 gagaatatcg tcatacagcc ctgtcccgac gtgcggatca aaaaagacga agggttcttt
     5221 cgccagttca gccaacgtta cattcgggtt atttgccagg ggatgatcgt gcgggatcat
     5281 cgccataagc ggttcatgga cgatgactgc gtgtgcaagc gtctccggta acgctgtgtt
     5341 acgcagcaat cccatatcca gcgttccttc aatgagcgga gcgatttgct cacgagtgtt
     5401 catttcgcgg gtttgtaaat ggacaccggg ataatcacgg cgaaacagcg ataacgtatc
     5461 ggacaccgcc cgaataaaag gagcggacga agtaaaacca atgcgcaact cccccgcttc
     5521 accctgatgc agtctttcag cgcgagcggc agcgtcatct accatagaca ggatttgccg
     5581 actatctgca agaaactgtt ttcctgctgc cgtcagcaat acactgcgat tagttcgtgc
     5641 cagcagtcgg gcaccaattt gttgctccag tgcctgaatc tgctgactta gcggtggttg
     5701 cgaaatattc aggcgggcag cggcgcgccc gaaatgcagc tcttccgcaa cagcaacaaa
     5761 gtaacgcaga tgacgaagtt caatattcat atttaaaaca tcttatttga gattattaat
     5821 atattagaca gaacaattcg attttcctaa cctatgtata agcctgatcc acaggcatat
     5881 ttagcaagga tttcaagtga gccgtactac aactgttgat ggcgctccgg caagcgacac
     5941 tgacaagcaa agcatttctc agccaaatca atttattaaa cgcggtacgc cgcaatttat
     6001 gcgcgtcacc ctggcgctgt tctctgccgg actggcaacc tttgcactcc tctattgtgt
     6061 gcagcctatc cttccggtgc tttcgcagga gtttggctta acccccgcga acagtagtat
     6121 ttcactgtcc atttccacgg cgatgttggc tattggtttg ctgtttactg gccccctatc
     6181 cgatgccatt ggtcgcaaac cagtgatggt cacggcgcta ctgttggcct ccatttgtac
     6241 gttatttcga caatgatgac cagctggcac ggcattttga ttatgcgcgc cttgattggg
     6301 ctttcgttaa gtggcgtggc agctgttggc atgacttatc ttagcgagga aatccacccc
     6361 agtttcgtgg ccttttcgat ggggttgtat atcagcggca actcaattgg cggcatgagc
     6421 ggacgcttaa ttagtggtgt cttcacggac tttttcaact ggcgaattgc tctggcggca
     6481 atcggttgtt tcgcgctagc ctcggcgttg atgttctgga aaatccttcc ggaatcacgc
     6541 cattttcgcc cgacttcgct gcgacctaag acgttgttta tcaactttcg tctgcactgg
     6601 cgtgaccggg gattaccgtt attgttcgca gaaggctttt tgctgatggg gtcgttcgtc
     6661 acgctgttta attacatcgg ctatcggttg atgctttccc cctggcatgt cagtcaggcc
     6721 gtggttggct tattatcgct ggcttatttg accggtacac ggagctcacc caaagccgga
     6781 accatgacca cccgctacgg gcgcggtccg gtgatgttgt tttcgacggg ggttatgctg
     6841 tttggtttac tgatgacctt attcagctcg ctgtggctga tctttgccgg aatgttactc
     6901 ttctcagcag gattcttcgc agcccactca gttgccagca gctggatcgg cccccgcgca
     6961 aaacgcgcta aaggccaggc ctcctcgctg tatctgttca gttactatct ggggtcgagt
     7021 attgccggga cgctgggtgg tgttttctgg cataactatg gctggaacgg cgtcggcgca
     7081 tttattgctc tgatgctggt cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg
     7141 cacgcctgaa aaataagccc ggactgcggt aactacacgt ccggacttat tgcttgctca
     7201 aaccaacgtt aatagccatc ctaaaataga cgaagcgcca gccaattccc gcagtgcgtc
     7261 tagcgtcatc aggattataa gtacccaaat aaacggattc attttgctgt gtgtcattta
     7321 ttactgatgc gcagttattc taccgctttg taagtagtaa aatagttaac ccgatcaaga
     7381 ctactattat tggtagctaa atttccctta agtcacaata cgttatatca atgctgtaat
     7441 ttattcagcg tttgtacata tcgttacacg ctgaaaccaa ccactcacgg aagtctgcca
     7501 ttcccaggga tatagttatt tcaacggccc cgcagtgggg ttaaatgaaa aaacaaattg
     7561 agggtatgac aatgaaaaaa gtattagctc tggttgttgc cgctgctatg ggtctgtctt
     7621 ctgccgcctt tgctgcagag actgcgacga ccaccaaagc agcgccggcg aaaacgacac
     7681 atcataaaaa acagcataaa gcagcacctg cccagaaagc gcaggcagct aaaaagcatc
     7741 ataaaaatac gaaagctgaa cagaaagccc ctgaacaaaa agcgcaggca gcgaagaaac
     7801 acgccgggaa acacagtcat cagcaaccgg caaaacctgc tgcacaaccc gcagcgtaag
     7861 ttttcaacag taatgctggc gcgccccctc gcgcctgaaa attacggtgc taagcgggta
     7921 acgtttagca ccgcctttag cggagggtag tatggtgggc cgttatcgct ttgagttcat
     7981 tcttatcatc cttattttat gcgcactgat tgccgcccgt ttttatcttt cctgactgta
     8041 gttatctgat tttactccca ctttcatccc gtcccgtcta tagtatttat gagggtttgc
     8101 ttttaataat cataattacc caccagagtg tgatatgcgt acaaccattg ctgtagtgtt
     8161 gggtgcaatt agtttgacgt ctgcttttgt gtttgcagat aaaccagacg ttgccaaatc
     8221 ggcaaacgat gaggtcagca ccctgttttt tggtcatgat gatcgtgtgc cagtgaatga
     8281 cacggcccaa tcaccgtggg atgcggttgg gcaactggaa acggccagcg gcaatttatg
     8341 tacggcgacg ctgattgcac ccaatctggc attaacggca ggacactgtt tattgacacc
     8401 tccaaagggt aaagcggata aagcagtggc gctgcgtttt gtgtcaaata aaggtctttg
     8461 gcgctatgag atccacgaca tagaaggctg cgttgatccg acactgggaa agcggttaaa
     8521 agcagatggg gatggttgga ttgtacctcc cgcagccgcg ccgtgggact tcggattgat
     8581 tgtgctacgt aatccccctt ctggcattac accgttgccg ttatttgagg gagataaagc
     8641 cgcgcttact gccgcattaa aatcggctgg ccgtaaagta actcaggcgg gataccctga
     8701 agatcatctc gatacgttgt acagtcatca aaactgtgaa gtgactggct gggcgcaaac
     8761 gtcggtgatg tcacatcagt gcgatacctt gccgggtgac agcggttcgc ctctgatgtt
     8821 gcataccaat gacggctggc aattaattgg ggtgcaaagt tcggctcctg ccgcgaaaga
     8881 tcgctggcgc gccgataacc gggccatttc tgttaccggt tttcgcgaca agctggatca
     8941 actgtcgcaa aaataatgtt caaacgctgc ccgactgtgc gggcagcgtg ttcatcaggc
     9001 aagtttcacc atgatcattc cagccaacag caagaccagg ccaatccagc ctttacgatt
     9061 taaccgttga ccaaacaaga tccaacctgc ggctaacgtg gcggcaatac caaacccgcc
     9121 ccacaatgca taagcgacag acaagtcgat ccctttaacg gcctgagaaa gcgcactaaa
     9181 ggcagccagc accgccgcca gggagagcaa gccaaatatt ttgcgacgaa agccgtcaga
     9241 aaatttcaaa aagacgttag caacgatttc cagcacgatt gccaatgcca gccaggcggc
     9301 gtgaacccat tcaaactgcg ccatggttca cctccagttc aggtttacgc gctttacggg
     9361 tacctgattt tatcaacaca atcccggcga ccagggtggt taacccggca attttcatca
     9421 gcgataaact ttcgtcgaat aacaaaacgc taaacaaggt aataaataaa ataccgatac
     9481 cttcccacag cgcataagct acgcctaagg cgatcttttt aacggcgaaa gagagaaata
     9541 tatacgatag agaaatcatc accagcatta aaataaagcc gccatttccc tcactgacgc
     9601 tcgcccattt cattgacagc gtaccggtaa tttctgtagc aatagccagg cctaataaaa
     9661 tccaataaat atacattgtc cttctcctgc aagagaatta ttttaatttt cgcttaattc
     9721 agcgaaatca aagtaaagtg gtcaagcccc gcgcataggg caagcttagg cagaagaaag
     9781 gactaaagcg cgttgcgcca gtgctgctca cctacgagca agatagtgga tgaggtacga
     9841 agatgggtaa atgttgaaaa taaggtcctg aacaaattgt ccataatatt acaattatcc
     9901 gcagtgttgc ttctcgtcat cgcggatgat aattgtcctc ggtagttgaa cacgcctgat
     9961 ttgtatcata gcttaagaat taactcaaaa tattttcact tctttacctg agcggtttga
    10021 ttttcgttat gatgacggag cgaaaaagac attattatta gcaaaggaag aaaaaacggg
    10081 gacaagcatg gcaaagccga tcatcacgct caatggccta aaaatcgtca ttatgttggg
    10141 aatgctggtc attattctct gcggtatccg ttttgccgcc gagatcatcg tgccgtttat
    10201 tctcgcatta tttattgctg ttattcttaa cccgctggtg caacacatgg tccgctggcg
    10261 agtgccgcgt gtactggcgg tgtcgatttt gatgaccatc atcgtgatgg cgatggtgtt
    10321 gctattagct tatctgggtt ccgcgctcaa cgagttgacg cggacgttac cgcaatatcg
    10381 caactctatt atgacgccgc tgcaagctct tgagccgttg ttgcaacgcg tagggattga
    10441 cgtctcagtt gaccagctgg cgcattacat tgatccgaac gcagcgatga cgttgctcac
    10501 caacttattg acgcagttat ctaatgccat gtcatcaata tttttattgc tgctgacggt
    10561 gctgtttatg ctgctcgaag tgccacaatt gcccggaaaa tttcagcaaa tgatggcgcg
    10621 tccggttgaa gggatggtgg cgattcaacg tgcaattgac agtgtttctc attatctggt
    10681 gctgaaaaca gccatcagca tcatcaccgg cctggtcgcc tgggcgatgc tcgccgcact
    10741 cgatgttcgc ttcgcttttg tctggggatt gctggccttt gcgcttaatt acatccctaa
    10801 tattggttca gttctcgcgg caatcccccc tatcgctcag gtactggtgt ttaatggctt
    10861 ctacgaagcg ttgctggtgc tggcgggata tctgctgatt aatctggtct tcggcaatat
    10921 tctggagccg cgcatcatgg gacgtgggct ggggctttcc acattggtgg tatttttgtc
    10981 gttgattttt tgggatggtt gttaggaccg gtgggtatgc tgctttccgt gccgttaaca
    11041 attattgtca aaattgcgct tgaacaaaca gcgggaggtc aaagcatcgc cgttctgtta
    11101 agcgatctca ataaagagtg acggcctccg cagaggccgt cagggttaca gagctttcag
    11161 gattgcatcc acgctggctt tggcgtcacc aaacagcatg tgggtgtttt ccttgaagaa
    11221 cagcgggttt tgcacaccag catagccagt gttcatcgaa cgtttaaaga caatcacgtt
    11281 ctgcgctttc cacacttcca gcacaggcat accagcaata ggactcttcg gatcatcctg
    11341 cgccgccggg ttaaccgtat cgttagcacc aatcaccagt acggtatcgg tatcagcaaa
    11401 gtcatcgttg atctcgtcca tttccagcac gatgtcatac ggtacttttg cttcagccag
    11461 caatacgttc atatgtccag gcaaacgccc cgcaaccggg tggataccga aacgcacgtt
    11521 gataccacga gcgcgcagtt tctcggtaat ttcagcgaca ggatattgcg cctgcgcgac
    11581 tgccatgccg taccccggag taatgatcac tgaatgggag tttttcagca gttccgctgt
    11641 ctcttctgcg gtgatttcgc ggtgctcgcc cacttcctga tcatcgccag tagaagagcc
    11701 gtcggtgccg aaaccacccg caataacgct gataaaggaa cggttcatcg ccttacacat
    11761 aatgtaagaa aggatagccc ccgaagaacc gaccagcgca ccggtcacaa tcagcaggtc
    11821 gttgctgagc ataaagcccg cagccgcagc cgcccagccg gagtacgagt tcagcatcga
    11881 caccaccact ggcatatctg caccaccgat ggaggcgact aaatgccagc cgaataccag
    11941 cgcaatggcg gtcattatta gcaatgccag cacttgcagg ccgacgctgt ccgtgcgaac
    12001 aaatacaatc agcagcagga aggaaacgac cagagccgcc aggttcattt tgtgacggtt
    12061 tggcagcatc aacggtttag acgaaatctt gccacacagt ttgccgaacg ccaccaccga
    12121 acccgtgaac gttaccgccc cgatgaagat accgaggaac acttccgtca ggtgaatatt
    12181 gaccagaatc ggtgccattc ccgcgtcatg atgcagatag ctgttaaagc caaccagcac
    12241 tgccgccaga cccacgaagc tgtgcaggat cgccaccagt tctggcattt cggtcatttc
    12301 aactttcttc gccagacgga taccaattgc cccaccaatg accatcgcca gcaagatcca
    12361 gccaacatta cccgtatccg gtccaaaaat ggttgcgatt aacgcaatcg ccatccctgc
    12421 gataccgaag ttgttaccct ggcgagacgt ttcatgtttc gaaagaccgg ccagactgaa
    12481 gataaacagg atcgcggcaa caatgtatgc agctgtaact aatcctccag acatatgtta
    12541 ccccttaatt tttgcggaac attttcagca tgcgctgagt cacggtgaag ccaccgaaaa
    12601 tattaatgct ggctataagc accgcgataa aactaaggaa gctaacccag ccgccctggc
    12661 caatctgcaa cagtgctccg acaacaataa tccctgaaat cgcgttggtg accgacatca
    12721 acggtgtatg cagcgcgtgc gatacattcc acaccacgta ataaccgaca acgcaggcca
    12781 gcgcgaaaac ggtgaagtgc ccaagaaact ctttcggcgc aacgcttgcc agccaaccga
    12841 aaaggatgat tgccagcgcc atcaacgcgt atttacgcca cggtgagcag gcacattttt
    12901 cctcagtttt cacttccggt gccgcttttt gtgccgcctg cggctgagct gatacctgaa
    12961 tcggcggtgc cggccaggta atttcgcccg cacgggtcac ggtcacgcgg cgaatcacca
    13021 catcatcaaa atcaacagtg atattgccgt ctttctcttt gcacaacagt ttcagcagat
    13081 taacgaggtt agtaccgtaa agctgcgagg attgcgtcgg cagacggccc ggaagatcgg
    13141 tataaccaat cactttgaca ccattttccg tagtgaagat ttcacccggc acggtgtatt
    13201 cacagttgcc accgttttgg gctgccaggt cgacaatcac actgcccgtc ttcatggagt
    13261 caaccatttc acgggtaatt agcttcggcg ctggtttgcc tggaataagc gcggtggtga
    13321 caatgatatc gacctctttt gcctgggcgg caaagagttc catttccgct ttaatgaacg
    13381 cgtccgacat cactttggca tagccatcgc cgctgcccgc ttcctcttta aaatccagct
    13441 cgaggaattc cgcgcccata ctttgaactt gttctttcac ttccggacgg gtgtcgaatg
    13501 cacgcacaat cgcgccgaga ctgtttgctg cgccaatggc ggccagacct gcaacacccg
    13561 caccaatcac catcactttt gccggtggca ctttcccggc cgcagtaatt tgcccggtaa
    13621 agaagcgccc aaattcatgt gccgcttcaa caatggcgcg ataaccggcg atgttcgcca
    13681 tcgagcttag tgcgtccagc gattgtgcgc gtgagatacg cggcacagaa tccatcgcca
    13741 tcacggtcac gttacgttcc gcaagttttt gcattaattc cggattctgc gcaggccaga
    13801 taaaactcac cagcgttgtc ccaggattca gtaacgcaat ttcatcatct aacggcgcat
    13861 tgaccttcag aatgatctct gactgccaga cgctattccc ttctacaatt tcagcgcccg
    13921 cttgcacaaa cgctttatcg tcaaaacttg ccagttgacc cgcgccgctc tctaccgcga
    13981 cggtaaaacc cagtttcagc agctgttcca ctgtttttgg cgttgctgca acacgggttt
    14041 cattggttaa ccgttctctt ggtatgccaa ttcgcatgat attcccttcc atcggtttta
    14101 ttgatgatgg tttgcctgtg tcaggagcca cacaagccgc tcatgtacga gctaaatgtt
    14161 actccgttaa aataaattag taacaaacgc cctataacgt actgaaaatt atgcctgtga
    14221 tctagcgcca aaaatcagta tttcggcgtg aactcgcaaa atattaacga ttcagccgtg
    14281 atagtgggat aaacacctta gaacgccgga taaagactga taattgtctt cgacggtcgg
    14341 gtaaaacgag acatcgcccc ggcacgattc actacttaac attaaattaa cttatacaat
    14401 tcagttgctt cagtagtaat gatgctgata cggctgtttt ttaagcatag acggtcattt
    14461 gagcaggatt aaaattggct taaggaatgt gatatgaaaa atgacgcaga cagttacacc
    14521 gtttaaatgc aataatcagc cacgtttctc gttaataaca ataccagtac ctggtttgcg
    14581 caaggcgaag gattattttt atgaagctta agaacaccct cctggcgtcg gcactgcttt
    14641 ctgctacggc attctccgtt aacgcagcaa cagaactgac accggagcaa gcggcagcgg
    14701 ttaaaccttt tgaccgtgta gtggttaccg gtcgttttaa tgctattggc gaagcggtga
    14761 aagccgtttc tcgtcgcgca gataaagaag gtgccgcctc tttttatgtt gtcgacactt
    14821 ctgattttgg taacagcggt aactggcgtg tggtcgctga cctctataaa gccgatgctg
    14881 aaaaagcaga agaaacaagt aatcgcgtaa ttaacggtgt tgtcgaactg ccgaaagatc
    14941 aggctgttct gattgaaccg tttgacacgg tcaccgtcca gggcttctat cgtagccagc
    15001 cagaagtcaa tgatgccatc accaaagcgg caaaagcgaa aggtgcctac tctttctaca
    15061 tcgttcgtca aatcgatgcc aaccagggcg gcaaccagcg tattactgca ttcatctata
    15121 aaaaagatgc taagaaacgt atcgtccaga gcccggatgt gatcccggca gattccgaag
    15181 caggacgtgc agctctggct gccggtggcg aagccgcgaa gaaagttgag atcccaggtg
    15241 ttgcgactac cgcatcacca agttctgaag tcggtcgttt ctttgaaacc cagtcatcaa
    15301 aaggcgggcg ttacaccgtc acgctcccgg atggcactaa agtcgaagaa ctgaacaaag
    15361 cgaccgcagc gatgatggtc ccgttcgata gcatcaaatt ctctggcaac tacggcaaca
    15421 tgaccgaagt ctcctatcag gttgcgaaac gtgccgcgaa gaaaggtgct aagtattatc
    15481 acatcacccg ccagtggcag gaacgtggta ataacctgac cgtcagcgca gatctgtata
    15541 aataactatt cagttactcc tataaggcgg cttgatgccg cctttcgcat ttttagtcac
    15601 aaatttcccc gcgaccattg catgcagaca catctcttcg taaaatcccg cgcttcagtg
    15661 gcaaactcca tttttatgcg ttttgcccta atatttattc ttcttatcac gttttaatca
    15721 ctggatatcg atggaaaaga aactgggact gagcgcactc accgcgctgg tattaagctc
    15781 aatgctgggc gcgggtgttt tcagtctgcc gcaaaatatg gcggcagttg ccagcccggc
    15841 agcactgctc atcggctggg gtattactgg cgctggcatt ttattgctgg cctttgccat
    15901 gctgatcctc acgcgcattc gtcccgaact tgacggcggt atctttacct atgcccgcga
    15961 agggtttggc gagctaatcg gcttttgttc cgcatgggga tactggctgt gcgcagtcat
    16021 cgccaacgtc tcctatctgg tgatcgtttt ttccgcgtta agctttttta cggacacgcc
    16081 ggaattgcgc ctgttcggcg atggtaatac ctggcaatcg atcgtcggtg catcggcttt
    16141 attgtggatt gttcactttt tgatcctgcg cggggtgcaa accgctgcca gcattaacct
    16201 ggtggcgaca ctggcaaaat tgttgccgct gggtctgttt gttgtgctgg cgatgatgat
    16261 gttcaaactg gataccttca agctcgactt caccggactt gtacttggcg tacccgtttg
    16321 ggaacaggtg aaaaacacca tgctgatcac cctgtgggtt ttcattggtg tggaaggtgc
    16381 cgtggttgtt tctgcgcgtg cgcgtaataa acgtgatgtt ggcaaagcga cactgctggc
    16441 ggttctctcc gctctgggcg tttacttgct ggtaacgctg ctttcgctgg gtgtggtggc
    16501 acgtcctgaa ctggctgaaa ttcgtaaccc gtcaatggcc ggtctgatgg tggaaatgat
    16561 gggaccatgg ggcgaaatca tcatcgctgc cagtttgatc gtttccgttt gcggcgcgta
    16621 cttaagctgg accatcatgg cggcagaagt tccgttcctg gctgccactc ataaagcatt
    16681 cccacgcatt tttgcgcgcc agaacgcaca agctgcgcca tccgcctcac tgtggttgac
    16741 caacatctgc gtgcaaattt gtctggtact catctggctg accggttccg attacaatac
    16801 gttgctgacc atcgcctcag aaatgattct ggtgccctat ttcctggtcg gcgcgttctt
    16861 gctgaaaatc gcaacccgtc cactgcataa agcggtaggt gtcggtgcct gcatttatgg
    16921 cttatggtta ttgtacgcct ctggtccaat gcacttgctg ttatccgttg ttctgtatgc
    16981 gccggggctg ctggttttcc tgtatgcgcg caaaacgcat acacatgata atgtactgaa
    17041 ccgtcaggaa atggtgctga ttggcatgct gttaattgcc tcagttccgg cgacctggat
    17101 gctggtggga taacgttcat cccatcgttg gatagcaaag gagataacga tgggtaaagc
    17161 ccagcccttg ccaatattaa ttactggcgg aggtcgtcgc atcggcctcg ccctcgcctg
    17221 gcatttcatt aatcaaaagc aaccggtgat tgtcagctat cggacgcact atccagccat
    17281 tgatggactg attaaggcag gtgcgcagtg tattcaggct gatttttcga ccaccgacgg
    17341 tgtgatggcg tttgccgatg aagtactaaa aagcacccat ggtctgcgtg ctattttgca
    17401 taacgccagt gcgtggatgg cggaaaaacc gggtgcgcca ctggccgacg tactggcttg
    17461 catgatgcag atccacgtta ataccccata cctgctcaac catgcgctgg aaagattact
    17521 gcgtgggcac ggacacgccg ccagcgatat cattcacttt accgattatg tggtggagcg
    17581 cggtagcgac aagcatattg cgtatgctgc aagcaaagcg gcactggata atatgacccg
    17641 ctcgtttgcc cgcaagctgg caccggaagt gaaagtgaat tctattgctc catcgctgat
    17701 cctgtttaat gaacatgatg atgccgaata tcgacaacag gcgctgaata aatcactgat
    17761 gaaaaccgcg cctggcgaga aagaagtgat cgacctggtc gattacttac ttaccagttg
    17821 ctttgtcacc ggacgcagtt tcccacttga tggcggtcgt catctgcgtt aatgcagctt
    17881 tatccagcat ataatcaaca cccatgcgct tatcccccaa cacgcaacag aaccaacaaa
    17941 agcggcgggc aagcgcatca ttccggtgaa ataccacagc gacaccaggt aaacaaaata
    18001 gggaataatc gaccacatac taaaaatgat ggttgcgcgt aaggcttcaa tgccgcgttc
    18061 gctggcaaca atataatgcg cgataagcgc aaaggtcggg aaaagtggaa tcagcccggc
    18121 gatataataa tttttcgttt ttgctaaaac accaatcaac agcactacca gcgcaccgag
    18181 cgcggctttg attaccagcc ccattttttt accttaacac ttccataaca agtcatcagt
    18241 agaatacctg atgaaaactt gtttagaaac gattgatagt aagtaaaaac agcgcggtgt
    18301 attgtgacgt ttttatatct accgtgaatg ttatgaacac tatcgtattt gtggaagatg
    18361 atgcggaagt cggttcactg attgccgcgt acctggcaaa acatgatatg caggttaccg
    18421 tagagccgcg cggcgaccag gccgaagaaa ccattttgcg agaaaatccg gatttggtgt
    18481 tactcgacat catgctacca ggcaaggacg gcatgaccat ttgtcgtgat ttacgcgcaa
    18541 agtggtctgg accgattgtt cttctaacct ctctcgatag cgatatgaac cacatcttgg
    18601 cactggaaat gggtgcctgc gactatattc tcaaaacgat gccccctgct gttttgctag
    18661 cgcgtttacg tttgcatttg cgtcagaatg agcaagccac actgaccaaa ggtcttcagg
    18721 aaacgtctct gactccctac aaagcccggc atttcggcac gttgaccatc gatcccatca
    18781 accgcgtagt caccctggct aacactgaaa tctcgctctc gacagctgat ttcgaattat
    18841 tgtgggaatt agctacccat gccgggcaaa tcatggaccg cgatgcattg ctgaaaaatt
    18901 tacgcggcgt cagttatgac ggactggatc gtaacgtgga cgtggctatt tcgcggttaa
    18961 gaaaaaaact gctcgataac gccgcagaac cttatcgcat taaaactgta cgtaacaaag
    19021 gctatctttt tgcgcctcat gcatgggaat aagcgatgaa aaaactgttt atccagtttt
    19081 acctgttatt gtttgtctgc ttccttgtga tgtctctgct ggttgggttg gtgtacaaat
    19141 ttaccgccga acgcgcgggc aaacagtcgc tggatgattt gatgaacagt tcgctgtatc
    19201 tgatgcgcag cgaattgcgt gagatccccc cacacgactg gggtaagacg ctgaaagaga
    19261 tggatttaaa tctctctttc gatctgcgtg tcgagccact gagtaaatac catcttgatg
    19321 atatttccat gcaccgactg cgtggcggcg aaattgtcgc cctggacgat cagtacacgt
    19381 ttttgcagcg tatcccgcgc agccactacg tgctggcagt tggtcctgtt ccttatcttt
    19441 attatctcca tcagatgcga ttgctggata tcgccctgat cgcttttatt gctatttccc
    19501 tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca ggatatgtta aaactggaag
    19561 cagcggcgca acgatttggc gatgggcatc tctatgagcg tatccacttt gatgagggtt
    19621 cgagctttga acgacttggt atcgcattta accagatggc ggacaatatc aacgccttga
    19681 ttgccagcaa aaaacagctt attgacggta tcgctcacga actgcgaaca ccgttagtgc
    19741 gcctgcgtta tcgactggag atgagcgata acctgagcgc cgccgagtcc caggcgttga
    19801 atcgtgatat cagtcaactt gaagctttaa ttgaagagct gctgacttat gcccgactcg
    19861 atcgcccaca aaacgagctt catcttagcg aaccagacct gccgttgtgg ctgtcaacgc
    19921 atctggcaga tattcaggca gtaacgcccg ataaaacggt acggataaaa acgctcgtgc
    19981 aaggccatta tgcggcgttg gatatgcgct taatggagcg cgtgctggat aatttgctca
    20041 ataacgccct gcggtactgc cattcaacgg ttgaaaccag cctgctactg tcggggaata
    20101 gagcgacatt aattgttgag gatgatggcc cagggattgc cccagaaaac cgcgaacata
    20161 tctttgaacc ttttgttcgc ctcgatccca gccgggatcg ctcaaccggc ggctgcgggc
    20221 tggggctggc aattgtccac tctatagcac tggcaatggg cggtacggtt aattgtgaca
    20281 ccagcgaact gggtggtgct cgcttctcgt ttagctggcc gttatggcat aacatcccgc
    20341 aatttacctc tgcctgacac tacgcgcacg atggtcaagt caataccatt gtgctataaa
    20401 ttaaggatgt tgtaactaaa gtggttaata ttatggcgcg ttacgatctc gtagaccgac
    20461 tcaacactac ctttcgccag atggaacaag agctggctgc atttgccgct catcttgagc
    20521 aacacaagct attggttgcc cgcgtgttct ctttgccgga ggtaaaaaaa gaggatgagc
    20581 ataatccgct taatcgtatt gaggtgaaac aacatctcgg caacgacgcg cagtcgctgg
    20641 cgttgcgtca tttccgccat ttatttattc aacaacagtc cgaaaatcgc agcagcaaag
    20701 ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga taacctttcg caagcagcgt
    20761 tggtcagtca tattcagcac atcaataaac tcaagaccac gttcgagcat atcgtcacgg
    20821 ttgagtcaga actccccacc gcggcacgtt ttgaatgggt gcatcgtcat ttgccggggc
    20881 tgatcaccct taatgcttac cgctcgctca ccgttctgca cgaccccgcc actttacgtt
    20941 ttggctgggc taataaacat atcattaaaa atttgcatcg tgatgaagtc ctggcacagc
    21001 tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg gacgcgcgag gagtggcaaa
    21061 gaaaactgga gcgagagtat caggatatcg ctgccctgcc acagaacgcg aagttaaaaa
    21121 tcaaacgtcc ggtgaaggtg cagccgattg cccgcgtctg gtacaaagga gatcaaaaac
    21181 aagtgcaaca cgcctgccct acaccgctga ttgcactgat taatcgggat aatggtgcgg
    21241 gcgtgccgga cgttggtgag ttgttaaatt acgatgccga caatgtgcag caccgttata
    21301 aacctcaggc gcagccgctt cgtttgatca ttccacggct gcacctgtat gttgcagatt
    21361 aacgcccggc tttcatactg ccgaccatct gttctggccg tacccagctg tcaaactcgg
    21421 cttcgctaag ataccccagc gcaagggccg cagcttttaa ggtcagccct tctttatgcg
    21481 cttttttggc gatctccgcg gctttgtcat aaccaatgtg ggtgttaagc gcagtcacca
    21541 gcatcagcga ttcattgagt aattgattga ttcgctcacg attcggttca atacccactg
    21601 cgcagtgttt gttaaaactt tccatgccat ctgccagcaa gcgcaccgat tgcaggaaat
    21661 tatggatcac catcggacgg aagacgttca gttcaaagtt accggaagcg ccacccatgt
    21721 tgatcgccac gtcgttcccc attacctgac agcagagcat ggttaatgct tcacactggg
    21781 ttgggttcac tttccccggc atgattgagc tgcccggctc attttccggg attgagattt
    21841 caccaattcc gcagcgcggg ccagaggcca gccagcggac atcattggcg attttcatca
    21901 gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac cagggcatca caggtcgcaa
    21961 gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca ggtaatgact gccagttcat
    22021 ctgctacgcg acgcgcatac tccggatggg tatttagtcc agtacccacc gctgtaccgc
    22081 caagagccag ttccgctacg tgaggcaggc tgtattcgat atgtttgaga ttatgctcga
    22141 gcatcgctac ccagccggaa atctcctgcc ctagtgttag cggcgtggcg tcctgcaagt
    22201 gggttcgacc gattttgacg atatcggcaa atgcacgcga tttttcactc agtgtctgtg
    22261 tcagggtttt aagctgcgga atgagttgct tgcgcagcgc cagcagcgcc gcaacgtgca
    22321 tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt cacgtcgtcg ttagggtgaa
    22381 ctttacgttc catcccgcgc acgccgccga gtaattcact ggcccggtta gccagcactt
    22441 cgttcatgtt catgttactt tgcgtgccgg agccggtctg ccagatagcc agcgggaatt
    22501 cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg ccgaatggcg ctcgctttct
    22561 cttcagacaa caatcctaaa tcttcattaa cttttgccgc tgcgcgcttg gttagcgcca
    22621 gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat gcggaaatgc tccagcgagc
    22681 gttgagtttg tgcgccccac agcttatctg ccgggacatc aatcgccccc atcgaatctt
    22741 tttcgctgcg tactgtattc atggcctgct cctcacctgt ttaatttttt ctttctgttt
    22801 tgctttcgtt aagcaactct tagctcactt attatttacc atttgataac aaatgtttgg
    22861 tctttcgtgc catgtaaaaa aaccgccccg aagggcgtaa tgccagtcag taaagcaact
    22921 g
//
LOCUS       DAJTNA010000054        22904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTNA010000054 DAJTNA010000000
VERSION     DAJTNA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22904
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.055"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..1196)
                     /gene="wcaD"
                     /locus_tag="OO161_001743"
     CDS             complement(<1..1196)
                     /gene="wcaD"
                     /locus_tag="OO161_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5462844.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILR"
     gene            complement(1171..2388)
                     /gene="wcaC"
                     /locus_tag="OO161_001744"
     CDS             complement(1171..2388)
                     /gene="wcaC"
                     /locus_tag="OO161_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5462845.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLINYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKYSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2385..2873)
                     /gene="wcaB"
                     /locus_tag="OO161_001745"
     CDS             complement(2385..2873)
                     /gene="wcaB"
                     /locus_tag="OO161_001745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5462846.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKSVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2876..3715)
                     /gene="wcaA"
                     /locus_tag="OO161_001746"
     CDS             complement(2876..3715)
                     /gene="wcaA"
                     /locus_tag="OO161_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR5462847.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKVCLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3808..5970)
                     /gene="wzc"
                     /locus_tag="OO161_001747"
     CDS             complement(3808..5970)
                     /gene="wzc"
                     /locus_tag="OO161_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5462848.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNMFPIFGAGWDRLMGRQNEAVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLQEKRQALEEEKAKLNGRVTAMPKTQQEIVRLIRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5973..6416)
                     /gene="wzb"
                     /locus_tag="OO161_001748"
     CDS             complement(5973..6416)
                     /gene="wzb"
                     /locus_tag="OO161_001748"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5462849.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6422..7561)
                     /gene="wza"
                     /locus_tag="OO161_001749"
                     /pseudo
     CDS             complement(6422..7561)
                     /gene="wza"
                     /locus_tag="OO161_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
     gene            7870..8019
                     /locus_tag="OO161_001750"
     CDS             7870..8019
                     /locus_tag="OO161_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462850.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            8220..9803
                     /locus_tag="OO161_001751"
     CDS             8220..9803
                     /locus_tag="OO161_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5462851.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(10077..11930)
                     /gene="asmA"
                     /locus_tag="OO161_001752"
     CDS             complement(10077..11930)
                     /gene="asmA"
                     /locus_tag="OO161_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5462852.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVDGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11952..12533)
                     /gene="dcd"
                     /locus_tag="OO161_001753"
     CDS             complement(11952..12533)
                     /gene="dcd"
                     /locus_tag="OO161_001753"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5462853.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12625..13266)
                     /gene="udk"
                     /locus_tag="OO161_001754"
     CDS             complement(12625..13266)
                     /gene="udk"
                     /locus_tag="OO161_001754"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5462854.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13584..16894
                     /locus_tag="OO161_001755"
                     /pseudo
     CDS             13584..16894
                     /locus_tag="OO161_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(17003..17851)
                     /gene="alkA"
                     /locus_tag="OO161_001756"
     CDS             complement(17003..17851)
                     /gene="alkA"
                     /locus_tag="OO161_001756"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5462855.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGRLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            17985..19337
                     /gene="yegD"
                     /locus_tag="OO161_001757"
     CDS             17985..19337
                     /gene="yegD"
                     /locus_tag="OO161_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5462856.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANCRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(19350..21290)
                     /gene="yegI"
                     /locus_tag="OO161_001758"
     CDS             complement(19350..21290)
                     /gene="yegI"
                     /locus_tag="OO161_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR5462857.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPVSKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVTGFMMPKVSGKEPIHMIYS
                     PGHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLAMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYSAHLTDCLWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(21287..22048)
                     /locus_tag="OO161_001759"
     CDS             complement(21287..22048)
                     /locus_tag="OO161_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR5462858.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAKLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGRGLELPLTPMVGEYANMTHFI
                     SDEDAVSRLETFTSTELAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFIGLAAATQE
                     QLDLLPKLLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(22045..22704)
                     /locus_tag="OO161_001760"
     CDS             complement(22045..22704)
                     /locus_tag="OO161_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5462859.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         5462 a         6061 c         5800 g         5581 t
ORIGIN      
        1 tttcgcaaaa taaacggaca gacgattaaa aatgcatatt ccgggctaaa tatcgaaccg
       61 gtaaaaaaca gcgataccgg agtaaaaaga tataattgca cgcgaaaatt gcggttatca
      121 ccaaaagcgt tgatcatcat ttttatcact ttccccatgt accacagcga taaaaacacc
      181 gcgaaccagg aaaaataaat aatcagcaga tacaaaccat tgtctatggt ttttccgaca
      241 tccgcaccgt taaatattcc gaatgatgcg acatattcat aaagtgagcc aaatctgact
      301 acaccgtcaa tatgggtcaa agaataaccg accatcacca gcggaccgac aatacgataa
      361 tacgatgacg atccttccgt acctaaatcg cccagacggg tggaaatata aggaaacgcg
      421 attaccacac caaccaggaa tactgccaga gatatcaatg ccaacggtaa ctttttctta
      481 atcgcctctt tattcagata ttgaaatgcc cactccagca aataaaacag gataaaggtc
      541 ataacccctg aaaacgatcc ggataatatt atccctgcga gaatcatagc atcggtttta
      601 ggcgttttga taccaaactg tttgatgctg agccaaattg agattaatgc cagagcgaaa
      661 aatgccggtt cgaaataaag tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagt
      721 acatagctgt tgctgtaaat cagatatttc gaaatcgact ccataatact gctgccgccg
      781 gtgaggataa tttgcgccat ctccaccgcc gccagcgcca ccaccagccc gaccaccaga
      841 taaaagaaac gtaatatctt gcgatggttg tgtggcgaaa ttgttttaaa acgaatactc
      901 cacaccatgc caataatgat cacaatatag acaaacagca tagttgaagt gacgtattta
      961 ctggcatcca gcgactgacc aaacagatag ttaaacgccg tgagtcccgc gccaatccct
     1021 aaggcaatca tcaatttttt aacgctgatg cgttctaaaa acagcagtaa caagacaggt
     1081 aaaaaagtga cgatggtgat ggggaaactt tcgccaagct gggcgatttt gacgttaacc
     1141 agcagataaa tcagcggcag caacaggtag ctacagattc tgatagaagt tgacatactc
     1201 ctccagcatc tgttgtccac tgtaggcggc gcggctgcgt tggctgaact cagccagcgt
     1261 ggtaccaaat atcgcctgcg cgatttccgg tttgcttaac tgcaccagtt gcagcacctc
     1321 ttcttcgctg acggttttac cgccggattt ttgcaacact tcccgcgccg catcgctatg
     1381 ggtggcaatc accggcacgc caatcgatag cgcctcacac aaaatcagcg ggtagttatc
     1441 gacgcgagaa ctgaacacca gcgcatccat ctgattgagc gcgctcatca acttgcgctt
     1501 gtcagtttca aagccgtgat taaccacgtt gccagcggtg aacggcgagt acttaccaaa
     1561 ggtatgtagt tcaattttgt cgcccagcgc catcatctcg cgtaccagtt gctggttagt
     1621 tttgccgtcg taacgcagat catgcgccac caccgcgatt ttcggcttgc cctgggtttc
     1681 gcgcaccgga ggcaagtccg ccagaatcgc ttcggttgcc atatcaatgc cattattgat
     1741 aatccggcaa cgccctggac cgtacaggct attgaaagcg tcagccacat gctggctggg
     1801 ggaaataaac tgacagccca gcgccagcat ctcacggaat aactggcgtt tgcccgccac
     1861 cagctggtgt gcgcgatcaa tcttcaccgg cggataatta attaaggtcg ggcatttctg
     1921 gcagcccgtt ttccagcctt cacaaccgtc ggtaaaggcg cagcgcccgg taacgctcca
     1981 gtggtcgtgc agcgtccaga ccagagtgac atccggtttg tggtttttca ccttttcgca
     2041 aaagcgcacc acgctcttaa gatttagcca gtagctgtgc agcacatgaa aatgcaggac
     2101 caccggaccc ggtgtacgag taatggtgcg atataactca ttgaaattgc caaacagatc
     2161 gcgattaaac agacgaaaca gggcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt
     2221 gatgacctgc ggatagttct gatggctgac gctctctttg ccgcctttgc cgtagccgta
     2281 gacaaaatgt gacgccagcc cctgttgcag cgcacgctgg tggagatcta acgccacacc
     2341 tgctgccccg ccttccgcca gtcgcacatt aaattgcaga atattcattt gattaccttc
     2401 actcgcgctt tttctcccac caccagcgcg ttgtccggga cagagtcaag caccacgctg
     2461 cccgcgccca cggtgacgtt gttaccaagc gtgatatcac caagaataat gacgttggca
     2521 ccgagttcga cgccgttgcc aatgtgtgga catgccatgt tatcggcacc acgattgccg
     2581 atagtgacgc cgtggcgaat ggtgaaatca tcccccgcca ccacgttttt attgatcact
     2641 acggcgtaac cgtgatggat agtaaagcgg cggccaatgg tcgcggcagc ctggatttca
     2701 taaccgaaaa agcattcggt gataatgcga tacagcacca gcagcggggc cgcccacaga
     2761 ttgttgagga cgctcttttt gcgccacacc gaacaaaaat gagcgacgcg ataggcaaga
     2821 accatgcagc acgggcgtaa actccagctg ttggcgcgca gatcttccag catctttagc
     2881 gcccccgaat accatcagcc agacgtttgc cgttacgcac cgacagcagc gtcagcaaag
     2941 tgcgccaggt catgcgttta ttgcggatct ggtagagggt aaacagctga tattttttgc
     3001 tggcgcggtc aaatttgtct ttgtgcttac ggtaaaagtg gaagtatccg gagaattttt
     3061 tcggtgacga ggtgatctgc atttcgccgt gattgatatg caggatctgc gtcgcctctt
     3121 ccactttcca cggttcgccg tactccacca ccatccgcag gaaaatgtcg taatcctgtg
     3181 ccgctttcag ttcggtatcg aataagcaca ctttgaaacg ccacgcccag gtaaaaacct
     3241 gattgccaat gatattgcgt ttgtagaaca ggcgacgtga atacggcgat ttgggataca
     3301 gtggtaagct tgccggttgg gaataaactt ctccctggca tacgtagtcg ttagcgtaca
     3361 aaaaggcgtg agtgaccagt tgctgtttat gggcgaggaa gacgctcaga cggttgggtg
     3421 tccattcgtc atcgtcatcg atcccggtaa tgtattcccc ttgcgccagc ataatcgcct
     3481 ggttacgtac cgcgcacgcc ccgctgttaa tgtcgttgtg aatgtaggtg atacgcggat
     3541 cgttgagggc agtgacgtac tgttgcagtt gttcccagga ggtggagcaa tcatccacga
     3601 tgatcatctc ccagttgctg tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca
     3661 gttgttggcg gttccaggtc ggcatataga ttgaaatcag cggattgtct ttcataggtt
     3721 gttctcccgg cttatgagct ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc
     3781 tacggggcgg tgcgaatgca ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta
     3841 atagccataa tcctgatacg cgctggcgcg gcggaagatg gagttcagaa tcaccccttt
     3901 caccggaata ccgttttgct caaagcggct cagactggtt tccacttctt tcaatgtgtt
     3961 gaccgcatag cgcgccacca ttaacgtggt tccagcgtga cgcccgacaa ttgctgcatc
     4021 ggtcactgcc agaatcggcg gcgtatcaat caacaccagg tcgtagtttt tactcgccca
     4081 gctcaccaat tcggcaaagc gttcgctcat caacagttca gaaggatttg gcggcacctg
     4141 accgcgcggg atcaggtcaa atttggcaat agaggtcggt ttagcagcgg tagtaatatc
     4201 gccctgacca atcagaattt ccgacaggcc attaacgtta ttggtgccca acagctcgtg
     4261 ggtgtagcct ttgcgcatat cgcagtcgat caacaacacc cgtttattgg tctggctgat
     4321 caccgccgcc aggttggcgc agacaaaggt tttaccgatt gacgggctaa ccccggtcat
     4381 catcaacaca ttgttctgcg cctgcatcat cgcgaagtgc aaactggtac gcagactgcg
     4441 gatggcttca atcgccagat cggttggatt ccccaccgcc agtagctggc tctgtttata
     4501 gcgtttaatc cctttgatgg ttttgacgct atcgcgcgct ttctgccatt ccgacagcgg
     4561 gatgctggca tagacgctga ttccgtgttc ttccagcact tgcgggcttt caatgccgcg
     4621 attaaacaac gagcgcagca gtacacccac gatagagagc atcaggccaa ggataatcgc
     4681 gccgaggata atcagccctt tcttcggttt tagcacacca ggctgggtga ttgccgggtc
     4741 aacaatgcgc acatcgccga cggtgctggc ttcggtgatt ttcagctcct gctctttatt
     4801 cagcagttgc atatagacct gctgaccaga ctcgacatcg cggatcaggc gcacaatctc
     4861 ctgctgggtt ttcggcatcg ccgttacgcg accgttaagt ttggctttct cttcttccag
     4921 cgcctgacgt ttctcctgca gcgtgcggta cgccgggtga actttggtgt acagcttgga
     4981 gatttccgct tctttaaagg tcagttcgtt caactgtgcg tcgatgttca ccatcgaatc
     5041 gagcaccgct ttcgcttcaa gcggcagatc aacagaatct ttatcctgac ggaaggcatt
     5101 cagtttgttt tcggcaacat cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa
     5161 agcgaggctt ttcgacgctt ccgccgattt gcgctcaata ttttgttcct gatagttacg
     5221 ggcgatgctg ttaagaatgt cgcggatctg ttcgcgatct tcaccggtat aagtcaggct
     5281 cagtacgcct gcgtctttgc cgttctccgt taccgtcagg ctgttttgca gttggttgat
     5341 catccccaac gtggagtatt tggtgacggt aaactcactg cccgggcggg cgtgaatggc
     5401 ttcaaccatc agcgtgacgc cttctttttt cagcatctgg cccgcttgcc cacgggcgct
     5461 aaagccgcca tcactgctca gggtgtagtt tttattgtcc agcacattaa gcgtaaacac
     5521 ctggtccgcc atctctttcg ggcggttaaa ggtggtcact ttcaccgcct cgttctgacg
     5581 tcccatcaaa cgatcccagc ccgcaccgaa aatcgggaac atgtttttgc tcaccgcaat
     5641 atcgaggtcg agatcgtcca ccgttttacc aagcaccaga cgcgaacgaa tcaactggat
     5701 ctcggcgtcc gatgcaggcg gtttgttggc taatgccgaa ccaatgtcct gcactaacga
     5761 attgccgctg ttttgctcga tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa
     5821 gaaggtgtaa accacggcac agagggcaaa tacagcggtg atgccaatca cccaccagcg
     5881 cgcttcaatg acggtgccga ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg
     5941 agcggcatgt tgttttactt tttctgtcat tcttatacct gctctgcgtt caatgcctgc
     6001 gcccactggc gggcagaccg ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt
     6061 ttgcgatacg gatcggggat ttcacattcg ttatcccagt gaccaaacag catcactttg
     6121 ccgcgcatct caggtgccat ctcgcataag cgttcgatat ggcgcttttc catggtcaaa
     6181 atcaggtcat agttgcgaca caggcggcgg ctgatttgtc gtgcacagtg gccttccaga
     6241 gacagttgat gttctgcggc gacgctgata gcggtaggat cagcgccctt accgaccagc
     6301 gcgccgagtc cagcggactc cactttcagt tccgggtgat aacgttgcag taagcgttcc
     6361 gccgtcgggg aacggcaaat attgccgaca cagacaacta atatgttgtt aaacatgacg
     6421 attaccagtt atgaatgtcg ctggctgtat ccgtcatgta acggacaccg ctaatagttg
     6481 gcagcaactg attgatcaga cggttccagc gggaaaccgg ggcggtggtg acatacacca
     6541 cgtcataagg ttgcaggcgg aattctgtcg ccatcaccag cgacgtcgca tcggacatat
     6601 ccagctggta gatattggca atcttgccgt tacgcccacc ctcgcctttc agcggacgaa
     6661 tgacaaagat gccgctggcg ttggaggtgg tcatgtcgat accttcggca ttgcccagag
     6721 cttcagtcag ggtcatgccg ctaaagtcca ttttcagggt gctcagtttc ttcacttcac
     6781 ccatcacaaa tactttcaga tcatcattac gcggcacgta gagaatatcg ccgggataaa
     6841 gcagtcagtt ctgattgagg tcgccgtttt gcatcagcgc ctgcaaagaa atgcgttctt
     6901 cacgaccatt gtgtgttagc accacgttgc gccagtcagc ggtgtcggtc aggccacctg
     6961 cggcgttgat ggcgtcgaga atggtcagtg gtacgttggt gatcgcctgt tgaccggatt
     7021 tattcacctg acctgagata taggcctttt gtgagcggaa ggcggcgata ttaacgtcca
     7081 cctgcgggtc agcgatgtac gttgctaagc gcccggtaat atcactgcgg atttcagcga
     7141 gcgttttccc cactacgtga accttgccga tatacggata aaacatagtg ccgtcaggct
     7201 gtacccagtt gccggtgtcg ctggagctgc ggtactgacc ggctggcgtg gtgagttccg
     7261 ggtgatccca gacggtgaca ttaagaacgt cccccggccc gacgcgatac tggtaattcg
     7321 cgatctcact ttccagcgtc atattggggc gcgctacatt cgggcgtggg cgtaattggt
     7381 caatcaggcg cggagtcagc ggataaacat tcaccatttt gtcgagatcg aaatcagcgt
     7441 cctgctgttt gatgacgtct ttgcccatcg tcgacatatt gctgcccgga agtactgtgc
     7501 aaccgcttat caaggttact gacaccaata atggcatcaa tttcattttg gatttcatca
     7561 ttgtttattt atcactttgg cagagtaatt atcctgtgca ctattaatag caatgtcgcc
     7621 atgcacattt accttgcagt taattgaata aaattttaac tggcatcagt cctaaaaaaa
     7681 ttgatttcat ccgcaggcta ttgacagaat aattcagact ggtctttcag gcatccagac
     7741 acgctaccgc ccctggcttt ttagctacca atacactgat ttagcttaat ttttaaaacc
     7801 ctctcaacat acagtcgttg atgagaaagg gttattgcgg aaattaactt ccgaatataa
     7861 ggtgacatta tggtaattga atattggctc tccaataatg caggaggaag tgttacagct
     7921 aacggaatag caggcaaggt aacaattcgg caattggcta tttttaagaa ttatattaag
     7981 tgtcattcaa tatggttttt aggagtttct ttaggttgac aatatttaat attgcgtctc
     8041 cacatgcgat atttcttaaa taatgtttta ttattaccac ttttaattca gggataatgt
     8101 gaggttatta ccctcaaata atatgagata aatagtgctg caacattgca tttttgcccc
     8161 gatttatcca tgatcgaatt gtgacatttg tcatacaacg aataggtttt gtacttacta
     8221 tggaatggat tgccgatccg tctatctggg ccgggttaat cacgctgatt gtgatcgaac
     8281 tggtcctcgg cattgataac ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga
     8341 agcagcgcga ccgcgcacgg gttaccgggc tgctgctggc gatgttaatg cgcctgttac
     8401 tgctggcgtc aatctcctgg ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt
     8461 ttacctttag cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag
     8521 ccacgatgga gctgaacgaa cggctggaag ggaaagacag caataatccc acacaacgca
     8581 aaggtgcgaa gttctggggc gtggtgacgc aaattgtggt actggacgcc attttctcac
     8641 tcgactcagt gattaccgcc gtcgggatgg tcgaccattt actggtcatg atggccgccg
     8701 tggttatcgc tatcagcctg atgttgatgg ccagcaagcc gttaacccag ttcgttaata
     8761 gccacccgac catcgttatt ctctgcttaa gcttcctgtt gatgattggc tttagcctgg
     8821 tggcagaagg tttcggcttc gtcattccga aaggctacct gtacgctgcc attggtttct
     8881 cggtgatgat cgaggcactc aatcagttgg ctatctttaa ccggcgacgt tttctttccg
     8941 ctaatcagac gctacgccag cggactaccg aagcggtaat gcgcctgctt agcgggcaaa
     9001 aagaagatgc ggaactggac gccgaaaccg cgtccatgct ggtggatcat ggaaaccagc
     9061 agatctttaa tccgcaggaa cggcggatga ttgagcgggt acttaatctt aaccagcgta
     9121 ccgtcagcag cattatgacg tcgcgccacg atattgagca tatcgatctc aacgcgccgg
     9181 aagacgagat ccgccagttg ctggagcgaa atcagcatac gcggctggtg gttaccgacg
     9241 gtgatgacgc agaagatttg ctcggtgttg ttcacgttat cgacctgcta caacagtctc
     9301 tgcgcggcga accgctcaac ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa
     9361 ccttgccgtt gttacctgcc ctcgagcagt tccgtaatgc ccgcacgcac tttgcttttg
     9421 tggtggatga gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc actgaaacca
     9481 ttgccggtaa cttaccgaac gaagtggaag agatcgacgc ccgccatgat attcagaaga
     9541 atgcggacgg ttcctggacg gcgaatggtc atatgccgct ggaggatctg gtgcaatatg
     9601 tgccgctgcc gctggatgaa aaacgtgaat atcacaccat tgccgggctg ttgatggaat
     9661 atttacagcg tattccaaaa cctggcgaag aagttcaggt gggggattat ttgcttaaaa
     9721 cgttgcaggt ggaaagccat cgcgtgcaga aagtgcagat tattccactg cgtaaagatg
     9781 gcgagatgga gtacgaggtg tgatggtatc ggccatttta cccaatagcc gaaaggaata
     9841 agatgaggga accgcgccag cggcgattta ccgccgggag cccgggttgc cagggtggtg
     9901 gcgattgagc caccctggca cgttcaccgg cttgagcgtt ccatagtagc gaggaacatc
     9961 aagtgaacgg aacagccttc gatcttacat attcagaata taatgatgcc gccacgaacg
    10021 cctgatacga cgctttacgc gtcttatcag gcctacagga ctggtcacct ggtaacctac
    10081 atcttctcca gcaacttctt cacatccttg ccattgcggg aatctttatt ccgctcggcc
    10141 cagtcattca ggcgacgttt cgcttcgtcc tgtagatgtt tgcgcagtaa ctgatccact
    10201 tgcaggctgt aattgagttg ctgccagttg ccataaaccc gcagcggtac tggcgtttct
    10261 ttcaggaaat caatcagttt actttccccg ttccagccat ccacgacccg aatatcaaac
    10321 tgggtatcgc aggtttgatc tgccagattc aacatgcctt cccctgtcag cgccagcaca
    10381 ggcgattgac cttgcatgtc gtttaacgtc acgacgccat ccttcaacgt caaatcggtg
    10441 gtaaagcggt caagccgcgt cacgttatca aagttttcag cggccttcac atcaccgcca
    10501 ttacgctcta ccgcttgctg aatcatctgc tggaagttca tcccttccat gcgcgtgtcg
    10561 gtcatttcga catgtgcctg tccttgccag ttgtggcgga atgcgtcggc atctatgtca
    10621 gcaccggaga aatcaccagc cagtgacatt tttccggtca acgaaatcgg atagttaaac
    10681 gccttcagaa tggtaccaat ctcaacgttt tccagccgtg gctggaagtt tatccgcgga
    10741 tttattgatg tcgcgtccag cgtgcccggc agtgaaacct gtccaccgtt aagcttgccc
    10801 tgcagttgag taatttccag caaacccgac ttgttggtca tttgcgtggc aacatctgta
    10861 aaattcattc cgcgccagcg cacgttactg gcctgcaaca aaatatcagc cgtaaagcct
    10921 tgcagtccct gatatgccgg ttcatcaata cgcgaagaaa tgaccgggcg cggcaatgtt
    10981 gattggctct gcccctgctg cgcggcaccg ttttcaccat tcgctgtttc attaagcggg
    11041 atgaggttgt caagattcag ttgcgggaat tgcagcctca gctgccattc cggtttctct
    11101 gtcagagtga cctgtgcttg cccgctcagc gtactgtcat tggcggtcaa gctgatttgg
    11161 ttaaatgaaa ggcgtttatg tgactcctgc cactgggcct ggaaactccc ctgcccctga
    11221 ataccttgtt ttggtaaatc ggcaccctgc aactgccagt taatttgttc aatagccgcc
    11281 gttaaatcat gcggataatc agaagcatcc accgtaccgt taagggatat tgtgagatcg
    11341 cgctgatcgc gattaacccg cccggagaac tcaaatgagc cacgatgttg gggatcttgt
    11401 tccatttgca ggcggatatt gcggattgtc acctgctcgt catcttcatg ctggaacacc
    11461 agcacgctgt ccgccacctt aagactggat atatcaaacg accatccgcg atcgtctgac
    11521 agatccggca aggtattgtc gcgcggtgca accggagcgt cttcactgcg caccgcttcc
    11581 gtctgcggcg tcagttggat cactgcccct tttagcatca cctgcttaac gctcagttga
    11641 tgactcagta gtggtaaaag cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc
    11701 tggcttgcgc cctgggcggt gagagacatt cgcccggaga ggatactaag ctgcggccag
    11761 acgtgccaac gcagtggccc gtcgagctgc aattgataac cgctacgtgc agcaacttgc
    11821 ttgaccatat agtcgcggaa atcattcgga ttcaccagca acactaacgc agataacccg
    11881 gccaccagca cgaccaggag tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca
    11941 atgggcctga attagtcttt atcgattcgg ctggctaccg cgccctgctg gttgcgatat
    12001 ttcgcatctt cacggcggtt gtaaggtcgc gccgccgggc cggaaagcgg ctcaaagctc
    12061 agcgcaccaa ttaacatgcc cggacgcagc gccagcggca gcttaccgga gttgtagaac
    12121 tccagcacaa tgcaaccaga ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc
    12181 agccccagac gcgccagtga ggaacgcccg tccagccagc ccaccagatc ggctggcagc
    12241 gtcaccgact ccagcgtcac cgccagcgcc agctctcctg ggtgaagata gaacgcctcg
    12301 ccctcgtcga gaacgatctc atcgctcatc acgcggtcaa gcgcggcgct cacttcatct
    12361 ttgggaccgc tcagatcgat aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg
    12421 cccaggcgta catccaccgt cgcgccgtta atacgctcca ctggcggacg tgggttgatc
    12481 gacaaacggc cttcatcaag ccaggcttca atatctcggt cacacagacg catggcattt
    12541 tctcctttcg cgcatcactc ccttaacgcc aattacgtca agggcatacc aggttatcac
    12601 tgaacggtac acaatttatc aagcttattc aaagaactga cttattttcg ctttcaatat
    12661 atcgatcgcg atgcggtttt tcccgccgcg cggcacgata atgtccgcat attgtttaga
    12721 aggctcaatg aattgcagga acatcgggcg cacggttttt tgatattgcg ccatcactga
    12781 atccattgaa cgcccacgct cgttaacgtc acgcttgatg cggcgcatca ggcagatatc
    12841 cagcggggta tcaacgaaaa tggagaagtt aagttcgtca cgcaaacgcg catccgtcag
    12901 caacaaaatg ccttcgagaa tgatgacctt cttcggctca accgtcaccg tttctttcat
    12961 acgcgtatgt tcaacatagc tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc
    13021 ttgtaaatgc tcaagcagca gactgtgatc catcgcgctg ggatggtcgt agttggtctt
    13081 aacgcgttct tccatcgaca gatggctttg atctttgtaa tagcagtctt cgggaattac
    13141 gccgatgtgt tcatcaccga cttgctcacg caattcacga taaagggtac tggcaataag
    13201 actcttgccg gaagccgatg cgccagcgat accgataatg acgcactgat gagactgatc
    13261 agtcatatat ttagcgacct gattaacctg gatgttagga agggggcgac gaagcgccaa
    13321 acgcggcaat tatagggatt tcatccgtct gataccagtc gaatagcgtt gccgcgcgct
    13381 cagagttaat tgttgacaaa gaattcccgg gggcaaatta cgttgatcag ttttatgtaa
    13441 ggtaaaaaat gttatacgca gttgcgcaaa ttatccgcct ttacgtcact ttatgagcaa
    13501 ttcgcatata aaatgtaaaa cttttgtact agcataaaca cagaaacgaa tactggcgac
    13561 caggtcttgc ggataaagcg gtaatgagca aacaatcaca gcatgtatta attgccctgc
    13621 cccacccgct gcttcacctg gtcagtttag gtttagtctc gtttatcttt acccttttct
    13681 cgcttgagct ttcgcagttt ggcacccaac tcgccccact gtggttcccg acatccatca
    13741 tgatggtggc gttttatcgc catgccgggc gcatgtggcc gggaattgcg ctaagctgtt
    13801 cgctgggaaa tatcgccgca tccatcctgc ttttttccac cagctcgctg aacatgacct
    13861 ggacgaccat caatattgtt gaagccgtgg tcggggcagt gctgctacgt aaattgctgc
    13921 cgtggtataa ccccttgcaa aatctggctg actggctgcg tctggcactc ggcagcgcca
    13981 ttgttccacc tctgttgggg ggtgttctgg ttgtcctgct gacgcccgga gacgatcctc
    14041 tcagggcatt tttgatatgg gtactgtcag aatccatcgg cgctctggca ctggtgccgc
    14101 tgggattgtt atttaaacca cactatctgc tgcgccatcg caacccacgg ttgctttttg
    14161 agtcgctgct cacgttagcc atcacactga cgttaagctg gctttcgatg ctgtatctgc
    14221 cgtggccttt tactttcatt attgtgctgt tgatgtggag cgccgtgcgt ctgccacgaa
    14281 tggaagcctt tttgatcttc cttaccacgg tgatgatggt gtcgctgatg atggccgcgg
    14341 atccctccct gcttgctacg ccgcgtacgt acctgatgag ccatatgccg tggctaccat
    14401 ttttgctgat cctgctgccc gccaacatca tgaccatggt gatgtatgcc tttcgtgcgg
    14461 aacgcaaaca catttccgaa agcgaaatcc actttcggaa cgcgatggaa tattccgcta
    14521 tcggtatggc gttagtgggc accgagggac aatggctgca aaccaacaaa gcgctctgcc
    14581 agtttctcgg gtacagtcag gaagagctgc gcggactcac ctttcagcaa ctgacctggc
    14641 cggaggatct caataaagat ctccaacagg ttgaaaagct gataagcggt gaaataaaca
    14701 cctattcaat ggaaaaacgc tactacaacc gcaatggcga tgttgtctgg gcgttgcttg
    14761 ccgtctcact ggtgcgccac acggatggca cgccgctcta ttttatcgct cagattgaag
    14821 acattaacga gctaaaacgc accgaacagg tcaatcagca actgatggag cgcatcactc
    14881 tggctaacga agcgggcggg attggcatct gggagtggga gttgaagccg aatattttta
    14941 gctgggataa gcggatgttc gagctgtatg aaattcctcc acatatcaaa ccgaactggc
    15001 aggtgtggta cgagtgcgtg ctgccggaag atcgccagca tgccgaaaaa gtgattcgtt
    15061 gcaatcacgc tcgcccttta aactggaatt tcgcattacc gtaaaagacg gtattcgcca
    15121 tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac gtctcctcgg
    15181 catcaatatg gatatgaccg aagtgaaaca gcttaacgag gcattgtttc aggaaaaaga
    15241 gcgcctgcac attacgcttg attccatcgg tgaagccgtg gtctgtattg atatggcgat
    15301 gaaaattacc tttatgaatc cagtggcgga gaagatgagc ggctggacgc aggaagaagc
    15361 gttaggtgtt ccgctcctga cggtgttgca tattactttt ggcgacaacg gaccattaat
    15421 ggagaacatt tacagtgccg acacctcacg ttccgcgatc gaacaagatg tggtgttgca
    15481 ctgtcggagc ggcggcagtt acgacgtgca ttacagtatt acgccgttaa gtactctgga
    15541 cggcagcaat attggttcgg ttctggtgat tcaggacgtt accgaatcac gcaaaatgct
    15601 gcgccagctg agctacagcg cctcccatga tgcactgacg catctcgcca accgcgccag
    15661 ttttgagaaa caactgcgta tcctgctgca aacggtaaac agtacgcatc agcgacatgc
    15721 actggtgttt atcgatcttg atcgctttaa agcggtgaat gacagcgccg ggcatgcggc
    15781 gggcgacgct ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc gctccagtga
    15841 cgtgctggcg cggctcggcg gtgatgaatt tggtctgctg ctgccagact gcaatgttga
    15901 aagcgctcgt tttatcgcta cacgtattat cagtgctgtg aatgactatc actttatatg
    15961 ggaaggccgg gtacatcggg taggtgccag cgccgggatt accttgatcg atgacaacaa
    16021 tcatcaggcg gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg
    16081 tggccggggc cgggtgacgg tttacgaacc gcagcaagct gccgcacata gcgaacgggc
    16141 ggcgatgtcg cttgatgaac agtggcggat gattaaagag aatcagttga tgatgctcgc
    16201 ccacggtgtc gcttcgccac ggatcccgga agcgcgtaat ttgtggctga tttcacttaa
    16261 gctctggagt tgcgaaggcg agattattga tgaacaaaca tttcgtcgta gcttcagcga
    16321 tccggcgctt agccatgctc ttgaccgccg ggtattccac gaatttttcc agcaggccgc
    16381 aaaagcggtt gccagtaaag gcataagcat cgccctcccc ctttccgttg ccggtttgag
    16441 tagcgccacg ctggtgaatg atctgcttga gcagctggaa aatagccctc taccaccacg
    16501 gttattacat ctgattattc cggctgaagc gattttagat cacgcagaaa gcgtgcaaaa
    16561 actgcggctg gcgggatgtc ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt
    16621 caactcgctg aaagcgaata tggcagatta cctgctactt gatggtgagt tatgcgccaa
    16681 cgtgcagggt aatttgatgg atgagatgct gattacgatt attcaggggc acgctcagcg
    16741 actcgggatg aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg atacgctttc
    16801 tggcatcggc gtcgatctga tttatggtga ggtgattgcc gatgcccaac cgctggattt
    16861 gctggtgaat agtagttatt tcgcgattaa ctgaggcgtg cctccccatc gcctgatgcg
    16921 acgctaacgc gtcttatcat gcctacaaat cgctcattcc ccaggccgga taaggcgctc
    16981 gcaccgcatc cggcgaccaa cgtcatgctt cgtctggttg ccagccttcc gtataccaga
    17041 tatgcaacag cgcataagaa cgccagggct tccagcgctc ggcataacgg cggatttgcg
    17101 ccggtgtcat tcccggaaat cgctgtttaa tcagataatc atccggcagg aaaacatctt
    17161 tcgcctgcca gccacgcaaa gcaaaataat tcgccgtcca gcgcccgata cccggaaaag
    17221 tttgcagcgt tttcatcgcc tgctccacat cgcccggtat tgtcattggt aaggtgccct
    17281 ccagcgccgc atttgccaga tgaatcagcg cctctgcccg tttcaacggc atacctaacg
    17341 cttttaatgc ctgcgggtct gctgctgcca gccgctgagg cgtggggaag cagatatact
    17401 ccggaaaatc atccagccgt tcgccataaa gctgtaccac tttggcagtc aattttgccg
    17461 ccatcgccac gctcaccagt tggcctaaaa tcgcccgcac gccctgctca aaagcatcaa
    17521 cactgccggg taaacgcaat ccgggccgcg ccgcgcctaa cctgcccaac gcaccgttaa
    17581 caatttgtgg gttacattgc agatcaaaca ggcggctcat tttcgccaga cactctgcgg
    17641 caactggttc taaacctgca cttaaattta tgtgcagagt atggcgggct atatccggaa
    17701 tagcagtcac cacgccgcga tattcgccca ccgccagact acgggcataa taactgtccg
    17761 cgaccgtttc cacaccgctc accgcacggg cggcgagaaa tcccaacatc cacgaccagt
    17821 catacggcgg ctgccagttc agggtataca tcgcatctcc ttattcatcc gctttcagca
    17881 taaacgttat tcagacgctg cgctttgctt tcatattccg gttgtcgcga cggcaacatt
    17941 tcgctaaagt cacgcccctt cttcaccggc atggggatta tttcgtgttt attggttttg
    18001 attacggtac agcaaactgt tcagtggcgg tcatgcgtga cggtaaaccg cacttgctaa
    18061 aaatggaaaa cgacagcacg ctgctgcctt caatgctttg cgcgccaacg cgtgaagcgg
    18121 taagcgaatg gctgtaccgc catcatgatg ttccggcaga cgacgatgaa acgcaggcgc
    18181 tgttgcgtcg ggcgattcgt tataaccgcg aagaagatat cgatgttacg gcgaaaagcg
    18241 tgcagttcgg tctttcctca ctggcacagt acattgatga tccagaagaa gtgtggtttg
    18301 tgaaatcacc aaaatcgttc ctcggtgcca gcggcttaaa accgcagcag gtagcgctgt
    18361 ttgaggatct ggtctgcgca atgatgttgc acattcgcca gcaggcgcag gcgcagctgc
    18421 cagaagcgat tactcaggcg gtgattggtc gcccgatcaa cttccagggg ctgggcggcg
    18481 atgaagcaaa cgcccaggcg caagggattc tggaacgcgc ggcgaagcgt gccggattca
    18541 gggacgtggt attccagtac gagccggtcg cggctgggct ggattacgaa gccaccttgc
    18601 aggaagaaaa acgggtgctg gtggtggata tcggcggtgg tacgactgac tgttcattgc
    18661 tgctgatggg accgcagtgg cgttcgcgtc tcgatcgtga agccagcctg ctgggtcaca
    18721 gtggttgtcg tattggcggt aacgatctgg atatcgccct ggcgtttaaa aacctgatgc
    18781 cactgctggg tatgggtggc gaaaccgaaa aaggcatcgc cctgccgatc ctgccgtggt
    18841 ggaatgcggt tgccatcaac gacgtacctg cgcagagtga tttctacagt agtgccaact
    18901 gtcgtctgct taacgatctg gtacgcgatg cccgcgaacc ggaaaaagtg gccctgttac
    18961 agaaagtctg gcgtcagcgt ttaagctatc gcctggtgcg tagcgcagaa gagtgcaaaa
    19021 ttgctctttc aagcgtagcg gaaacccgcg cctcactgcc gtttatcagc aatgaactgg
    19081 ctacgctgat tagccagcga gggctggaaa gcgccctcag ccagccactg gcgcggattc
    19141 tggaacaggt gcaactggcc ctggataacg cccaggaaaa accggacgtt atctacctga
    19201 ccggcggtag cgcccgttct ccgctgatta aaaaagcgct ggcagaacag ttgccaggca
    19261 ttccgattgc aggcggcgat gactttggct ccgtcaccgc cgggctggca cgctgggcgg
    19321 aagtggtgtt tcgttaattg aaaaatggct cagcagcggc ttaaatctgc ctgcgcctga
    19381 gccagttttt ctgctgcctg acgtaacggt tccatcaacg gcatggtccg tgctggagca
    19441 tgaaggcgga atcgctgcaa ctctgtcgcg ccgactgtca acgccgattc cagccgatgt
    19501 cgtttggcgg tcattttcgc cattaccgct tgtctgtctg ctggcgttat cgcttcattc
    19561 ggcctgaaca caaaacggcg ttcacagctc gctttccagt cgataaccgc ctgggtcaga
    19621 tggtcgccaa accctttcac ttgcttaacg ccacgacggg taacatctgc ggctgtttca
    19681 ataccaaaag accgtaacgc cgctttacgc gcaggaccaa cgccgggaat agaggcaaca
    19741 tcaataaaaa atccctccag aaacttctgc ttctgccgtt cccttgcggt gtcgtgaagt
    19801 gctgccagag cacgtttttc ttcttccggt aacccgagca tttcgtcctt cattttttcg
    19861 agcatcgtcc gtttggcgat aaaaccttcc agcccgccca actgttggat ctggctgact
    19921 aaatggtcat agtccatttt cgcgcgatta aatgcctcac ggcgttgctg aacttctgct
    19981 ttgtacgctt tgcttgtcag actgccaata atccagatag ccgacagcac aggaaccaaa
    20041 ataatataac gcggttctgc ctgaaggccg caaagcaaca gcgataacgc tgacagtgcg
    20101 atctcaatca gaatgatgta ttcgcgccgt aacaggccta aaggaagcgg cctgccagtc
    20161 ggttggaaat gatcgggtaa tggcaattgc aatgctggcg gtgctactga cgccatcacc
    20221 atcgcccaga ctttcgccag cacaaaatca ccgctggtgg taatgacctc ttcgccgaga
    20281 tcaataaaat agataacgcc ttgattatcc agcgcacacc acaggcagtc agtcaaatga
    20341 gcggagtaaa catgcattgc cgaaacggta catttcttta actgttggcg tagtaaatca
    20401 agtgccgcta cccacgcttt tgccgtcgga cgcccggttg ccacgccact ttccgtaaac
    20461 gcctgctgaa acatggcttc aacatcgccc ggtaacatcg ccagcggaat agatcgtggc
    20521 ggcggtttta aaccacgtcg ctgattatct gacgcgtagg cataacggaa atgggcaata
    20581 tccgtctcca gcgcattacc cgcatcagag ataagcggca caccggaata aggatgccga
    20641 ccaccaaaca agacgtgaaa aatcaacaag gcaaggccaa aattatcgtg attttctgta
    20701 cgttcaaagc cgacaaatga cgacaaggtt tgtagctctg gcggcgtaaa atgcgacacg
    20761 ccgacttcgc ataaatgcag tgtgccattg gcgttaatct gaaaggagtc gctatcaatc
    20821 aacaccactt tgctgtcgcg acctaccata aaactgttct ggtttacgtc acccacgacg
    20881 tgcccgtgct cgtgaaccgt agcaaaagat gaagcaatat tgcgcgcaac atagagtaga
    20941 aaatcccacg cacaatgagg gtaactctgg cgacgatgtc ccgggctata gatcatatga
    21001 atcggttctt taccagaaac ttttggcatc ataaaaccgg taacttttcc gcctcgtcca
    21061 ccgtgaagcg ttgcctgcgg ccaggcgaca taattcaaca actgcgcgtc agctgtcgca
    21121 gccataaagg caagtttgtc ctgttttgag acgggtggcg gcgtgtgata aatcttggcg
    21181 acgctatcga caaactcctc gatatcataa accgcgcctt caccaccttt gcccagttca
    21241 cggcctggcg taacgcactc accagtagca gtatataaag tgggtttcat ggtaaccaca
    21301 gcgccagtgc caacgtttta tcatcatcgg tacgctcgtt taccgccggg ctggacaaaa
    21361 actgctttaa caatttaggt aataaatcga gttgttcctg cgttgctgcc gccagtccaa
    21421 tgaaaaacgg ggtaaagaaa ggcacatgcg gagaattatc cagcatattt aacgccagtc
    21481 gctgaatacc atccgtaaat gctgcaactt tatgcgcaag ctcagtgctg gtgaaagttt
    21541 ccagtctgga aacagcatct tcatcgctaa tgaaatgcgt catattggca tattcaccca
    21601 ccatcggcgt gagcggcagt tccagaccac gaccaaaatc caccactaca ccgccgtcgc
    21661 caatttgcat aatcaatgtg ccattagccg atgaaatcaa tcccagaaac gtgcaggcaa
    21721 aatcgcgaac tgctaatgct ttggcttctg cctcggcaaa aagtcgttga cgaatggtca
    21781 gtaccatatt tgtggcgaga acatcattaa gccccaattc accgtcctgt actttttgca
    21841 ccataaacgc catcgcttca ttgacggcga gcttcgcgcc ttctcctccc tgcgaaacgc
    21901 tacctgcgcc atcagcaagg aacatcacca gtaatggctg ctggtcattt agccaggcaa
    21961 cctgcatctg gcaggcatcc tgacaaggta aatcagcgct aatgtgcgac gttccaacag
    22021 cggatgcata aaccagacgc cagctcacac tgacgtccag ccttttggcg cttccagcac
    22081 aacttccgtt cccggcgtgg agcgggaaac cgaacgcagc gaactggata accagctaaa
    22141 cagttcacgg aattgtaatc cctgcagaga caaaggctga cggacgctga tttgggccag
    22201 cgtcttcata tccgccccct gaacaccgat ggaaaagaaa gcaaaccttt tatcttcttc
    22261 cccccgaaac actttgttgg ctgcagcctg ccactcatcg gttggtgcac catcggtaat
    22321 caggaaaatc cacgggcgat aatacgaaat accatttgca cgatactcac gtttccgctc
    22381 ctctaccata tccagggctt tggtaatggc cgcacccatt ggtgtatcgc cctgggcaaa
    22441 caggatgggc ggaaaaaaat tagcggcgct ggtaaagggt tgttccacat gcaccgggcc
    22501 gaacgtcaca atgccgagtt ccactctttt taaagccagc ggatcagcaa gcagttcatc
    22561 acgaaaggta accaatccgg cgttaagttc attaataggt ctgccactca tcgagccaga
    22621 aacatccagc agcagaatgc aggggcaacg tggttccggg ttactggcaa aatcgctggt
    22681 ggcaaacgtg atttgttcgc tcataagaaa tcctttctta gagaattatt gaggtccggt
    22741 atcataccga ctgcaataat gaaataagag caaacgaaga aactttcaga attaggcccg
    22801 gcggcgcaga aaattttgct attcgcctcg caagaacgga gagccgtcga ttgacactaa
    22861 gggcggagtg acataatttc aggagtgagg gttagggaga ggtt
//
LOCUS       DAJTNA010000055        22767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTNA010000055 DAJTNA010000000
VERSION     DAJTNA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22767
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.056"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..334)
                     /locus_tag="OO161_001761"
     CDS             complement(<1..334)
                     /locus_tag="OO161_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5462860.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGE"
     gene            complement(415..1842)
                     /locus_tag="OO161_001762"
     CDS             complement(415..1842)
                     /locus_tag="OO161_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5462861.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSVLGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPVEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1839..2546)
                     /locus_tag="OO161_001763"
     CDS             complement(1839..2546)
                     /locus_tag="OO161_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5462862.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTCGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2709..3686
                     /locus_tag="OO161_001764"
     CDS             2709..3686
                     /locus_tag="OO161_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5462863.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDSNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3756..4238)
                     /locus_tag="OO161_001765"
     CDS             complement(3756..4238)
                     /locus_tag="OO161_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5462864.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4473..7055
                     /gene="leuS"
                     /locus_tag="OO161_001766"
     CDS             4473..7055
                     /gene="leuS"
                     /locus_tag="OO161_001766"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5462865.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPIWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7070..7651
                     /gene="lptE"
                     /locus_tag="OO161_001767"
     CDS             7070..7651
                     /gene="lptE"
                     /locus_tag="OO161_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5462866.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSPAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7651..8682
                     /gene="holA"
                     /locus_tag="OO161_001768"
     CDS             7651..8682
                     /gene="holA"
                     /locus_tag="OO161_001768"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5462867.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8684..9325
                     /gene="nadD"
                     /locus_tag="OO161_001769"
     CDS             8684..9325
                     /gene="nadD"
                     /locus_tag="OO161_001769"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5462868.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9349..9960
                     /locus_tag="OO161_001770"
     CDS             9349..9960
                     /locus_tag="OO161_001770"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5462869.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVDNA"
     gene            10220..10537
                     /gene="rsfS"
                     /locus_tag="OO161_001771"
                     /gene_synonym="rsfA"
     CDS             10220..10537
                     /gene="rsfS"
                     /locus_tag="OO161_001771"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5462870.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10541..11008
                     /gene="rlmH"
                     /locus_tag="OO161_001772"
     CDS             10541..11008
                     /gene="rlmH"
                     /locus_tag="OO161_001772"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5462871.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11039..12940
                     /gene="mrdA"
                     /locus_tag="OO161_001773"
     CDS             11039..12940
                     /gene="mrdA"
                     /locus_tag="OO161_001773"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5462872.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     FRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12943..14055
                     /gene="mrdB"
                     /locus_tag="OO161_001774"
                     /gene_synonym="rodA"
     CDS             12943..14055
                     /gene="mrdB"
                     /locus_tag="OO161_001774"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5462873.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14066..15154
                     /gene="rlpA"
                     /locus_tag="OO161_001775"
     CDS             14066..15154
                     /gene="rlpA"
                     /locus_tag="OO161_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5462874.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGLGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTLATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15294..16505
                     /gene="dacA"
                     /locus_tag="OO161_001776"
     CDS             15294..16505
                     /gene="dacA"
                     /locus_tag="OO161_001776"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5462875.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16616..16879
                     /locus_tag="OO161_001777"
     CDS             16616..16879
                     /locus_tag="OO161_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5462876.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16980..17621
                     /gene="lipB"
                     /locus_tag="OO161_001778"
     CDS             16980..17621
                     /gene="lipB"
                     /locus_tag="OO161_001778"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5462877.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17880..18833
                     /locus_tag="OO161_001779"
     CDS             17880..18833
                     /locus_tag="OO161_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5462878.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIISSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19042..20007
                     /gene="lipA"
                     /locus_tag="OO161_001780"
     CDS             19042..20007
                     /gene="lipA"
                     /locus_tag="OO161_001780"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5462879.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLSQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20108..20311)
                     /gene="tatE"
                     /locus_tag="OO161_001781"
     CDS             complement(20108..20311)
                     /gene="tatE"
                     /locus_tag="OO161_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5462880.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAVAKKGADVDLQAEKLSHKE"
     gene            complement(20440..21228)
                     /locus_tag="OO161_001782"
     CDS             complement(20440..21228)
                     /locus_tag="OO161_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5462881.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGLLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21321..21704
                     /gene="crcB"
                     /locus_tag="OO161_001783"
     CDS             21321..21704
                     /gene="crcB"
                     /locus_tag="OO161_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5462882.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21759..21968)
                     /gene="cspE"
                     /locus_tag="OO161_001784"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21759..21968)
                     /gene="cspE"
                     /locus_tag="OO161_001784"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5462883.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22143..22679)
                     /gene="pagP"
                     /locus_tag="OO161_001785"
     CDS             complement(22143..22679)
                     /gene="pagP"
                     /locus_tag="OO161_001785"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5462884.1"
                     /translation="MFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQPEHYDLYI
                     PAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDSWNKWEPIA
                     GYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYI
                     PGTYNNGNVYFAWMRFQF"
BASE COUNT         5814 a         5709 c         5631 g         5613 t
ORIGIN      
        1 cttccccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
       61 agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata
      121 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
      181 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttata ccggtataca
      241 tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa
      301 gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt aagtgcatta
      361 actgactact tttttatatt acttgccggg ctttccgcac attaacgtcg actgtcaccc
      421 gaacagatgg agtattttcc ctatcaaacc ggcaaagata aaaatgtaga aaataatttt
      481 gatgacgccc agacagcccg gcccaccatc gtcaacgggc attggctgcg gttgcggctc
      541 cggttcaaca gtttgcttct cggtaaaatt cgccgcctga attaataatc gacgaataat
      601 taatgcttcc actggacaac gatcttctgc atcaagacgg cttacgatcg gcattaatga
      661 ttcttctgtc ggttggctgg cctgcactaa ttgtttggca actaaatata atggccataa
      721 gagatgttct gtatcacgcg tgagtgagat ccgcggctta cgtcctttgt gcaaaaccat
      781 ctcgaccagt gaacgcccca gcacagagag tttttcatcg ctgagtattt cgtataaatg
      841 aaaacgcgta cgggcgctgt cgccggggac ataaagtagc tgtttaccct gatcgttaac
      901 cagttcacga gcttcaatca tcatcaccac taacgggtaa taatcgaccc gatgctgacg
      961 gcaccattgc aatattaaag catccgcctg ggtggaggga tactcgcgcc agtagtcaca
     1021 aagttctgcc agcaagcttt cgccttctcc acaatagacg cgttgcgcgt attcataata
     1081 acgcgcatct tcattatcag gttcagcttc ccgccacgac cgggcgtaat ccagaagttc
     1141 ggggatatcc aggcgacctg cgctatacca gcggagtaaa tttctgtgca gttttgcatc
     1201 atcggggatg atcactgcgc gatgttgcgc taaatacaat gtcagccatt ctggatgcga
     1261 tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg tcattttgca cggcaacggg
     1321 cagatgcaga atggtctgga agttaaaaac attccccttc gcaatggcat ataaaaagga
     1381 ttccagttct tcttcgtcta tttccccgac gttttcctcc tgcaaccacc gcaatctttc
     1441 tgctaataac accacgcact cgaaggataa atgggcagtg ttcatggcga ttgtgcacag
     1501 cgaccagcgt aattcatcaa tctcttccat cgagcaataa tttaattgct gaatgaatcg
     1561 ctgccaggcg gagggcagaa aacgttcgct atcagaggca agtaacgcgc gaaaggcgag
     1621 cagaatttca tgttctgcta cctcatattc ttctggttgc caaacagatt tagcaggcga
     1681 ctgcgcaatc cgtagcgcct cctcatacgc ctgacgaagt tgtttaaaac cctgcggatc
     1741 ggtttctgga tgaaaggacg gtaacagcgc cagataagcg cggcggataa tatcgacgtc
     1801 agtcgtttcc tctatatcga ggatcttcca gcaatttttc ataagtcgct catatcctta
     1861 agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc aggacaaggt agggtaatac
     1921 gcatcatcac tggcaaataa ttgataaaac atatcattgc cggaatcgtc gtattcttta
     1981 cgcgctaaga attcgcgtaa taaaggcaga tgatcatctt cgggagactg ccaatattgc
     2041 cgaccacagg tataaccggc aaagtattgc cgccagttac tataataacg tagagcccgt
     2101 agatgaatac gcgactggat ccatagactt tcttcttcac tcaaccagtt attaagtacg
     2161 cccattcgac ttaaaaaacc catccggaca taatcccatg ctttaattcc tccctcacca
     2221 cagcattcgg ctgtgctggc gacaaattgc gcataagcct gaccttgctc atttaattca
     2281 gcaagatagt cacgccattc acatggagaa taacgaaacc agcggtgata aaaccctgcc
     2341 aaccgagcgg catgaccgtt ctcgatcatt cggtgaattg tgcgcagcag gtcatcacgg
     2401 tcatcaattc cccatgagga agaaaggtca aaaaagcatg tacctaaata cattttgggg
     2461 gatgaatgcg aaggtaaaca gcattcattg cgtatttcca tcggagtgga aagtgcaaac
     2521 aatatttttt gcgaccccat gtccatataa tttatcctta aacattcatt taatgaatgt
     2581 aaactacatt tcgagactca gttatttata cttttctgac gcgacataat acgcataaaa
     2641 taaggcttac gcaaaaatgg aacacataaa atataatatg ctttatctac aaccataagg
     2701 gaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
     2761 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
     2821 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgccg
     2881 agcaaggtca ttgtgaagca caatattcct taggacaaaa gtatactgag gataaaagcc
     2941 gtcataaaga taatgagcat gccatctttt ggctgaaaaa aactgcccta caaggccata
     3001 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagactcc aattataaag
     3061 aagcggttgt ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
     3121 ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga ctatgcgctg gcatttttct
     3181 ggtacaaaca agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt
     3241 atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
     3301 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
     3361 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
     3421 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
     3481 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
     3541 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
     3601 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
     3661 ttcgtgagac tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc
     3721 ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
     3781 aactggtcat gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
     3841 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
     3901 accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg
     3961 atatctgcca gctcctgcca taagctttct ttaatcaccc gcataaagac gctgtcagat
     4021 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tcttacagct
     4081 cgcgtcagct catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
     4141 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac
     4201 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
     4261 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
     4321 taaattaatg tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
     4381 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
     4441 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
     4501 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
     4561 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
     4621 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgctaccag cgtatgctgg
     4681 gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
     4741 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
     4801 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
     4861 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
     4921 ataagaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
     4981 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
     5041 agtggtttat caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg
     5101 atcactggcc agacaccgtt aaaagcatgc agcgtaactg gatcggtcgt tccgaaggcg
     5161 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
     5221 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
     5281 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
     5341 ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg
     5401 ttcacccatt aacgggcgaa gaaattccca tttgggcagc aaacttcgta ttgatggagt
     5461 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
     5521 ctaaatacgg cctgaacatc aaaccagtta tcctggctgc tgacggctct gagccagatc
     5581 tttctcagca agccctgact gaaaaaggcg tgctgttcaa ctctggcgaa ttcaacggtc
     5641 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
     5701 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg
     5761 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc
     5821 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
     5881 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
     5941 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
     6001 aaggtatgct ggattccgaa gcggctaact actggctgcc ggtggatatc tacattggtg
     6061 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
     6121 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggttc
     6181 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
     6241 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
     6301 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
     6361 cacaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
     6421 ctccggctga tatgactcta gaatggcagg aatccggtgt ggaaggggct aaccgcttcc
     6481 tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa aggtgatgtt gcggcactga
     6541 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaacgatcg
     6601 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
     6661 tggagctgat gaacaaactg gcgaaagcac caaccgatgg cgagcaggat cgcgctctga
     6721 tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc gttcaccccg cacatctgct
     6781 tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg
     6841 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
     6901 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
     6961 gccaggaaca tctggtagca aaatatcttg atggcgttac cgtgcgtaaa gtgatttacg
     7021 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
     7081 ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
     7141 tgataccacg caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa
     7201 cgggccatta agccctgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
     7261 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
     7321 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
     7381 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
     7441 ttcgttcttt gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
     7501 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
     7561 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
     7621 tgcacgcgtc tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc
     7681 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
     7741 ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt cgaagaacac
     7801 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
     7861 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
     7921 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgatga cctgctgttg
     7981 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt
     8041 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
     8101 gttgctgcgc gcgcaaaaca gctcaactta gaactggatg acgcggcaaa tcaggtgctc
     8161 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
     8221 ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
     8281 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcacattg
     8341 catattcttc agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta
     8401 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
     8461 gcgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
     8521 aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
     8581 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
     8641 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
     8701 tctgtttggc ggcacctttg atccagtgca ctatggtcat ctaaaacccg tggaaacgct
     8761 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
     8821 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga
     8881 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
     8941 gcaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
     9001 tggtcaggat tcactgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
     9061 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
     9121 ccagcaatgg ctggaagatc atttgacaca taaccaggaa gatcttcacc ttcagcctgc
     9181 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
     9241 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtac tgacttacat
     9301 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
     9361 ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc tttacagcgg tcatgcgccc
     9421 acccccctga ccgcgcgcgg tattgagcaa gcgcaaaatc tgcatacgct gctacatggt
     9481 gtttcctttg atctggtttt atgcagtgaa ctggaacggg cacagcatac cgcgcgactg
     9541 gttctcagtg accgccagct ccccgtgcaa atcatccctg aactcaacga aatgtttttt
     9601 ggcgactggg agatgcgaca tcatcgcgac ctcatgcaag aagatgccga aaactatagc
     9661 gcgtggtgca atgactggca gcatgcaatc cccacgaacg gtgaaggatt tcaggcattt
     9721 tcgcaacgtg tggaacgctt tatcgcaagg cttagtgaat ttcagcacta tcagaatatt
     9781 ttagtcgtca gccatcaggg tgtactgagt ctgttaatcg cccgtttaat tggcatgcct
     9841 gccgaggcta tgtggcattt tcgcgttgac caggggtgtt ggagcgccat tgatatcaac
     9901 caaaaattcg cgacgctacg cgtcctcaat agccgtgcca tcggggtcga taatgcatga
     9961 cttttctgtt tttttacggg taagccgcaa cgaccattga cagacccggg caggctgata
    10021 ttctccgcag ccagactttt ttcgccagac acgactttgt agaaattgtt ttacaaaaat
    10081 ggcgatgcaa tctgcggcgc ggggtgggat gatagcccac tttcgaaagc cgattcggcg
    10141 acaattgtcc cgaaatcgcc tctggttcag gtatactgac agaccatttt tatctatttg
    10201 attcacccag ggggaaaact tgcagggtaa agcactccag gattttgtta tcgacaaaat
    10261 tgatgacctc aaaggtcagg acatcatcgc cttagacgtt caaggcaaat ccagcatcac
    10321 cgactgcatg atcatctgta cgggtacgtc cagccgtcat gttatgtcca ttgctgacca
    10381 cgttgtgcag gagtctcgcg cagcgggcct gttaccgctc ggcgtagaag gtgaaaacag
    10441 cgccgactgg attgtcgtgg atttgggcga tgtgattgtc catgtcatgc aggaagagag
    10501 ccgtcgcctg tatgaactgg aaaaactctg gagttaatgc gtgaagctgc aacttgtcgc
    10561 cgtgggaacg aaaatgccgg actgggtaca aaccggtttt accgagtacc tgcgtcgttt
    10621 tccgaaagat atgcccttcg agctgattga aattccggcc ggaaaacgcg gcaagaatgc
    10681 ggacatcaag cgcatactcg acaaagaggg tgagcagatg ttggcggccg caggcaaaaa
    10741 ccgcattgtc accctcgata ttccaggcaa gccctgggat acgccgcagt tagccgctga
    10801 gctggaacgc tggaagctgg atggtcgcga cgtcagtcta ctgattggcg ggcctgaagg
    10861 gttgtcgcct gcctgtaaag cggcggcgga gcagagctgg tcgctgtcgg cgcttaccct
    10921 cccccatccg ctggttcgcg tgctggtcgc agagagtctg taccgggcgt ggagcatcac
    10981 caccaaccat ccttatcacc gtgagtgata agggagcttt gagtagaaaa cgcagcggat
    11041 gaaactacag aactcttttc gcgactatac ggcagagtcc gcgctgtttg tgcgccgggc
    11101 gctggtcgcc tttttgggga ttttgctgct gaccggcgtg cttatcgcca acctgtataa
    11161 tctgcaaatt tttcgcttta ccgactacca gacccgctct aatgaaaacc gcattaagct
    11221 ggtgcctatc gcgcccagcc gcggcattat ttacgaccgt aatggtatcc ctctggccct
    11281 caaccgcact atctaccaga tagaaatgat gccagagaaa gtcgataacg tgcagcaaac
    11341 gctggacgct ttgcgcagcg tggttgatct gaccgatgac gatattgctg cattccgaaa
    11401 agagcgcgca cgttcacacc gtttcacctc tattcccgtg aaaactaacc tgaccgaagt
    11461 acaagtagct cgctttgccg tcaatcagta ccgttttccg ggggttgaag ttaaaggcta
    11521 taaacgtcgt tactatcctt acggttcggc gttgacccac gtcatcggct atgtgtcgaa
    11581 aatcaacgat aaagacgtcg aacgcctgaa taatgacggc aaactggcca actatgcggc
    11641 aacgcatgat atcggtaagc tgggcattga gcgttactat gaagacgtgc tgcacggtca
    11701 gaccggttat gaagaggttg aagttaacaa ccgtgggcgt gttatccgcc agttaaaaga
    11761 agtaccaccg caagccggac acgatattta cctgacgctg gatctcaaac tccagcaata
    11821 tattgaaacg ctgctggcgg gtagccgcgc agctgtggta gtcaccgatc cgcgtacagg
    11881 tggggtgctg gcgctggttt ccacgcctag ttatgaccca aacttgtttg ttgacggtat
    11941 ctccagcaaa gattattccg ccttgttgaa cgacccgaat acaccgctgg tgaaccgcgc
    12001 cacacagggg gtttatcctc ccgcgtctac agttaaaccc tatgtggcgg tttcggcatt
    12061 gagcgccggg gtgatcacgc gcaatacgac gctgtttgac ccaggctggt ggcaactgcc
    12121 aggttcggaa aaacgttatc gtgactggaa aaaatggggc cacgggcgtc tgaatgtcac
    12181 aagatcgctg gaagaatctg cggatacctt cttctatcag gtggcctacg atatggggat
    12241 cgatcgcctc tccgaatgga tgggtaaatt cggttatggt cattacaccg gtatcgacct
    12301 ggcggaagaa cgttccggca acatgcctac ccgcgaatgg aaacagaaac gctttaaaaa
    12361 accgtggtat cagggtgaca ccattccggt tggtatcggt cagggttact ggacagcgac
    12421 cccaatccag atgagtaagg cactgatgat cctgattaat gacggtatcg tgaaggttcc
    12481 gcatttgctg atgagcaccg ccgaagacgg caaacaggtg ccatgggtac aaccgcatga
    12541 accgcccgtc ggcgatattc attccggtta ctgggagctg gcgaaagacg gtatgtacgg
    12601 tgttgctaac cgccctaacg gtacggcgca taaatacttt gccagcgcac cgtacaaaat
    12661 tgcggcgaaa tctggtaccg ctcaggtctt cggtctgaaa gcgaacgaaa cctataatgc
    12721 gcacaaaatt gccgagcgtt tacgtgacca caaactgatg accgcttttg cgccatacaa
    12781 caatccgcaa gtggctgtag ccatgattct ggagaacggc ggcgcgggtc cggcggttgg
    12841 tacgctgatg cgtcagatcc tcgaccacat tatgctgggt gataacaaca ccgatctgcc
    12901 tgcggaaaat ccagcggttg ccgcagcgga ggaccattaa tcatgacgga taatccgaat
    12961 aaaaaaacat tctgggataa agtccatctc gatcccacaa tgctgctgat cttactggca
    13021 ttgctggttt acagcgccct ggttatctgg agcgccagcg gtcaggatat tggcatgatg
    13081 gagcgtaaaa tcggccaaat cgcgatgggt ctggtcatca tggtggtgat ggcgcaaatt
    13141 cctccacgcg tttatgaagg ctgggccccc tatctctata tcatctgtat tattttgctg
    13201 gtggcggtag atgctttcgg tgccatctct aaaggtgctc aacgctggct ggacctcggt
    13261 attgttcgtt ttcagccgtc ggaaattgcc aaaatagccg taccactgat ggttgcgcgc
    13321 tttatcaacc gcgacgtttg cccaccgtcg ttgaagaaca caggcatcgc gctggtgctg
    13381 atatttatgc ccacgctgct ggtggctgca cagcctgacc tgggaacatc aatcctcgtt
    13441 gcgctttccg gtctgtttgt actgttcctc tctggcctta gctggcgtct gattggcgtc
    13501 gcagtagtgc tggtggcggc gttcattcct attctgtggt tcttcctgat gcatgattac
    13561 cagcgccagc gcgtaatgat gctcctggac ccggaatcag acccactcgg cgcgggctat
    13621 cacattattc agtctaaaat tgctattggc tccggcggat tacgcggcaa aggctggctg
    13681 catggcactc agtcacaact tgaatttcta cccgaacgcc ataccgactt tatcttcgcg
    13741 gtactggcgg aagagctggg attagtgggc attttgattc tgctcgctct ctacattctg
    13801 ctgatcatgc gcgggctgtg gatagccgcc agagcgcaaa ccacctttgg tcgcgtcatg
    13861 gctggcggat taatgctgat attattcgtt tatgtcttcg taaatattgg tatggtaagc
    13921 ggtattctgc cggttgtagg ggttccgctc ccactggtca gttatggagg atcagcgcta
    13981 attgtgctga tggctgggtt cgggattgta atgtcaatcc acacccacag gaaaatgttg
    14041 tcgaaaagcg tgtaagaggt gcgcaatgcg taagcagtgg ctcgggatct gcatcgcggc
    14101 aggaatgctc gcggcatgta caagcgatga tggtcagcaa cagacggtaa gtgtaccgca
    14161 gcctgcggta tgtaacggcc ccatagttga aattagcggg gcggacccgc gtttcgaacc
    14221 actgaacgcg acggcaaatc aggattacca gcgcgacggt aaaagctaca aaatcgtgca
    14281 ggatccgtct cgatttagcc aggcgggact ggcggcaatc tatgatgccg aaccaggcag
    14341 taacctgacg gcctctggcg aagctttcga tccgacacag ctgacggcgg cccatccaac
    14401 gcttccgatc cccagctacg ccagaatcac taacctggct aacgggcgaa tgatcgtggt
    14461 gcgcattaat gatcgcggcc cttacggcaa cgaccgcgtt atttcgcttt ctcgcgcggc
    14521 agctgaccgt cttaacacgt caaacaacac caaagttcgt atcgatccga ttattgtcgc
    14581 ccaggatggt tcgctttctg gtcttggtat ggcttgtacc acagtcgcca aacagactta
    14641 cgccctgcct gcacctcccg atttaagcgg tggcgcggga acaagttcag tgtctggccc
    14701 gcagggtgac attcttccgg tcagtaattc gacgctaaaa agcgaagatc cgaccggcgc
    14761 gccggtaacc agtagcggtt tcctcggcgc accaacgacc ttagcgcctg gtgtactgga
    14821 aggcagcgaa ccgacgcctg cgccacagcc cgttgttaca gctccgtcga caacgcttgc
    14881 aacctcgcct gcaatggtga caccgcaagc cgcctcgcaa agcgccagcg gcaactttat
    14941 ggtgcaagtc ggggccgtta gcgatcaggc tcgtgcgcaa cagtaccaac agcaactggg
    15001 acagaagttc ggcgtccccg gtcgcgtaac tcaaaatggc gcggtctggc ggatccagct
    15061 tggccccttc gccagcaaag ccgaagccag taccttgcag caacgtttgc aaaccgaagc
    15121 ccaattacag tcatttatta ccaccgcgca gtagcgtaaa gcaggcatct gatgtgtcaa
    15181 tttctgtaaa tgacgttaac aaagtcacgc ggaaagtcag atgcctgccg gtagtgcatt
    15241 tgctatagta gggcactttt tttaattcca tcacggatgt cgtagttcag accatgaata
    15301 ccattttttc cgctcgtatc atgaagcgcc tggcgctcac cacggctctt tgcacagcct
    15361 ttatctctgc tgcacatgcc gatgacctga atatcaaaac tatgatcccg ggtgtaccgc
    15421 agatcgatgc ggagtcctac atcctgattg actataactc cggcaaagtg ctcgccgaac
    15481 agaacgcaga tgtccgccgc gatcctgcca gcctgaccaa aatgatgacc agttacgtta
    15541 tcggccaagc aatgaaagcc ggtaaattta aagaaactga tttagtcact atcggcaacg
    15601 acgcatgggc caccggtaac ccggtgttta aaggttcttc gctgatgttc ctcaaaccgg
    15661 gcatgcaggt tccggtttct cagctgatcc gcggtattaa cctgcaatcg ggtaacgatg
    15721 cttgtgtcgc catggctgat tttgccgctg gtagccagga cgcttttgtt ggcttgatga
    15781 acagctacgt taacgccctg ggcctgaaaa acacccactt ccagacggta catggtctgg
    15841 atgctgatgg tcagtacagc tccgcgcgcg atatggcgct gatcggccag gcgttgatcc
    15901 gtgacgtacc gaatgaatac tcgatctata aagaaaaaga atttacgttt aacggtattc
    15961 gccagctgaa ccgtaacggc ctgttatggg ataacagcct gaatgtcgac ggcatcaaaa
    16021 ccggacacac tgacaaagca ggttacaacc ttgttgcttc tgcgactgaa ggccagatgc
    16081 gcttgatctc tgcggtgatg ggcggacgta cttttaaagg ccgtgaagcc gaaagtaaaa
    16141 aactgctgac ctggggcttc cgtttcttcg aaaccgttaa cccactgaaa gtaggtaaag
    16201 agttcgcctc tgaaccggtt tggtttggtg attctgatcg cgcttcgtta ggggttgata
    16261 aagacatgta cctgaccatt ccgcgtggtc gcatgaaaga tctgaaagcc agctatgtgc
    16321 tgaacagcag tgaattgcat gcgccgctgc aaaagaatca ggtcgtcgga actatcaact
    16381 tccagcttga tggcaaaacg atcgagcaac gcccgctggt tgtgttgcaa gaaatcccgg
    16441 aaggtaactt cttcggcaaa atcattgatt acattaaatt aatgttccat cactggtttg
    16501 gttaaaaatt gaacacttga aagtgtaatt tccgtcccca tatactaagc atcagtaaaa
    16561 aaactcccgc cttctggcgg gagttgctat ttaattacgt tacgccggag ctgacatgaa
    16621 aaccaaactt aacgaactgc ttgaattccc tactcctttt acttacaaag ttatggggca
    16681 ggcgttacct gagctggttg atcaggtggt tgaagtggta cagcgccatg cgccaggtga
    16741 ctacacccca acggtaaaac caagcagcaa aggcaactac cactcggtat ctatcactat
    16801 caacgccact catatcgagc aggttgaaac actgtatgaa gaactgggca aaatcgatat
    16861 tgtccgcatg gttctgtaac tcgcttctcc gttacccggc tcctggtcgg gtaactcccc
    16921 tctcctcgct gtgatatact tttcccccac ttttactcat tctccacgga gatgccgttt
    16981 tgtatcagga taaaattctt gtccgccagc tcggtcttca gccttacgag ccaatctccc
    17041 aggctatgca tgaattcacc gatacccgcg atgatagtac ccttgatgaa atctggctgg
    17101 tcgagcacta tccggtattc acccaaggtc aggcaggaaa agcggagcac attttaatgc
    17161 cgggtgatat tccggtgatc cagagcgatc gcggtgggca ggtgacttat cacgggccgg
    17221 gacaacaggt gatgtatgtg ttgcttaacc tgaaacgccg taaactcggt gtgcgtgaac
    17281 tggtgacctt gcttgagcaa acagtggtga ataccctggc tgaactgggt atagaagcgc
    17341 atcctcgggc tgacgcgcct ggtgtctatg tcggggaaaa gaaaatttgc tcactgggtt
    17401 tacgaattcg acgcggttgt tcattccacg gtctggcatt aaacgtcaat atggatcttt
    17461 caccattttt acgtattaat ccttgtgggt atgccggaat ggaaatggct aaaatatcac
    17521 aatggaaacc cgaagcgacg actaataata ttgctccacg tttactggaa aatattttag
    17581 cgctactaaa caatccggac ttcgaatata ttaccgctta attccataca tcaatggccc
    17641 aatttacact gggtcattac tcataatacc tgctaatgct ttacatctcc ttcgcgtttg
    17701 tatattttct ttatcacttg cagaaatgcg gtgccacgcc ttgctccgta actcacccca
    17761 ttgattaacc tattgaaata gttgattatg gtgagcaaaa gcgttccgtt ccaattagac
    17821 ataagattat tgtaattttt taaatattat ttttccatga ataaaattgg agtaagtgcg
    17881 tggatagtaa taatcaaatt gaaccctgtt taagcagaaa gtcgtcagaa ggtaaaccac
    17941 aaatatttac aaccttacga aatattgctc ttaaccttct gactattttt gaagctgtat
    18001 atgtacataa agggatcgtt aatgcagcga aagtgcttaa tctgcccccc tcggcaatca
    18061 gtcagtctat tcagaaactg cgcgttatat tccctgaccc attgtttatt cgcaaaggcc
    18121 agggtgtcac tcctaccgca tttgcgatgc atctacatga gtatatcagt cagggccttg
    18181 agtccattct tggcgcgctg gatatcgaag gaagctatga taagcaacga acgataacta
    18241 ttgcgaccac tccctcggtc ggagccctgg tccttcctgt catctatcgg gcgattaaaa
    18301 ctcactatcc gcagctttta ctgcgcaacc cgcccatcag cgacgcggaa aaccaactca
    18361 gtcagtttca aaccgatctc atcatcgata acatgttttg caccaatcgt acggtgcaac
    18421 atcatgttct gttcaccgac aatatggtgt taatttgccg cgagggaaat ccactactct
    18481 ctttagaaga tgacagagag actatcgaca acgctgcgca tgtacttctg ttaccggaag
    18541 agcaaaattt cagcggtctg cggcagagag ttcaagaaat gtttccggac cggcaaatca
    18601 atttcaccag ctacaacatt ttgacaatcg ctgcactggt tgccaacagt gacatgttag
    18661 cgattatttc cagccgtttt tataacctgt ttagccgctg ctggccgctg gaaaaactgc
    18721 cttttccgtc cttaaataag gagcaaatag atttctctat ccactacaac aaattcagcc
    18781 tgcgtgatcc catcctgcac ggagtaatag atgttatccg taatgcattt tgaaaaagta
    18841 ttgttacagc cagatcacag cgcagaacga taaaatggca caatccaaca acaattttac
    18901 attttcgcga ccgctttggc tgctttcagg tccgtttcaa tgatatactg ccagtcgtta
    18961 attcaaaaat agttgataat tacaacaatc tattgaattg aaacgctttc cttcgtaatt
    19021 cgcaactgga acacgcacgc tatgagtaaa cccattgtga tggaacgcgg tgttaaatac
    19081 cgcgatgccg ataagatggc ccttatcccg gttaaaaacg tagcaataga gcgcgaagcc
    19141 ctgctgcgca agccggaatg gatgaaaatc aagcttccag cggactctac acgtatccag
    19201 ggcatcaaag ccgcaatgcg caaaaatggc ctgcattctg tctgcgagga agcctcctgc
    19261 cctaacctgg cggaatgctt caaccacggc acagcaacgt ttatgatcct cggcgctatt
    19321 tgtacccgcc gttgtccgtt ctgtgatgtt gcccacggtc gcccggtagc tcctgatgcc
    19381 aatgaaccag tgaaactgtc gcagaccatt gccgatatgg cgctgcgtta tgtggttatc
    19441 acctccgttg accgtgatga cctgcgcgat ggcggtgccc agcactttgc ggattgcatt
    19501 actgccattc gggaaaaaag cccgcaaatc aaaattgaaa ctctggtgcc ggatttccgt
    19561 ggtcgtatgg atcgtgctct ggatattctg actgcaacgc caccagatgt gttcaaccat
    19621 aacctggaaa acgtaccgcg tatttaccgt caggtacggc ctggtgcaga ttacaactgg
    19681 tcgctgaagc tgctggaacg ctttaaagaa gcgcatccgg aaatcccgac caagtctggt
    19741 ctgatggtgg gactgggtga aaccaatgaa gaaattattg aggtaatgcg cgacctgcgc
    19801 cgtcatggtg tgacgatgtt aacgctgggg caatatttgc agccaagccg ccatcacctg
    19861 ccggttcaac gttacgttag cccggatgag ttcgacgaaa tgaaagccga agcgctggcg
    19921 atgggcttta cccatgctgc atgcggtccg tttgtccgct cttcttacca cgccgatttg
    19981 caggcgaaag ggatggaagt taagtaatcc ggtaataatt acttacatct gtctgataaa
    20041 aaacccgctt tttgaagcgg gttttttgta tcgaacaaga tattgaggga gcgtcctgct
    20101 cgccacgtca ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt
    20161 tcgccacagc atcatcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt
    20221 ctccgcccag cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta
    20281 ctagcagttt ggtaatacta atctcaccca tagatacctt cttgacatat aaccggcaac
    20341 agaacgcgcc ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca
    20401 atctgtaaca aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc
    20461 aaagcgacgg ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt
    20521 cacttccgcc ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg
    20581 atctataatc cggctttgcc cgatattttt gttcccgcac tcgcccgccg ccaccatata
    20641 acaggtggta tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcag
    20701 cccgcgaacc caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag
    20761 cgccagctct ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccttccac
    20821 ctccagtaac ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa
    20881 tgcatcatag agatgcagtt tggcataacg ggcgacgatg tttcctgcct gaagtgccac
    20941 cagcatattc catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat
    21001 catgttacgt ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc
    21061 tgatttaacc gatagatctg catcatgatc atcgcgtgcc agcaatgctt ccggcaggac
    21121 aaacagcgat acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt
    21181 cttttcccac acagatgtaa cagcaaactg tcctgcagca accagcattg ccacctcctg
    21241 tcagagtcgt aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa
    21301 tcaaatagca ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta
    21361 cgggaagcgt ggcgagatgg ctgttaagta tgcgatttaa cccactgcat caggcgattc
    21421 cgttggggac gctgacagca aacctgattg gggcattcat cataggaatg ggattcgcat
    21481 ggttcagcag gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt
    21541 gtggcggtct aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg
    21601 gtcgctttgg ctgggcatta ctgaacgttt tcgtcaacct tctggggtct tttgccatga
    21661 cagcactggc tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaacc
    21721 cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca
    21781 gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga
    21841 gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt gctgccgtct
    21901 tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc
    21961 ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc ggttaccagt
    22021 acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata aaagtcgcgt
    22081 tatttttgat gttacttatt ttagctattg attttaaaga agttactaaa acttcatttg
    22141 tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta ggtacccgga
    22201 atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg cagtagaacc
    22261 gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa tcccagacct
    22321 aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta tccggcaatc
    22381 ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg ccagtttcct
    22441 ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt atagcgatcg
    22501 gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat atataaatca
    22561 taatgttcag gctgttgcca ggttggtgca atattttctc taaacgttgt catccactca
    22621 tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa ggaaaagata
    22681 gcgacatatt ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg
    22741 ctccagtggc ttctgtttct atcagct
//
LOCUS       DAJTNA010000056        22667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTNA010000056 DAJTNA010000000
VERSION     DAJTNA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22667
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.057"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(130..609)
                     /gene="folK"
                     /locus_tag="OO161_001786"
     CDS             complement(130..609)
                     /gene="folK"
                     /locus_tag="OO161_001786"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5462885.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO161_001787"
     CDS             complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO161_001787"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5462886.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSTRRRTRRPRKRAPRREGTA"
     gene            complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO161_001788"
     CDS             complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO161_001788"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5462887.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO161_001789"
     CDS             complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO161_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5462888.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO161_001790"
     CDS             complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO161_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5462889.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO161_001791"
     CDS             complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO161_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5462890.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4982..7411
                     /gene="hrpB"
                     /locus_tag="OO161_001792"
     CDS             4982..7411
                     /gene="hrpB"
                     /locus_tag="OO161_001792"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5462891.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEYHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7613..10129
                     /gene="mrcB"
                     /locus_tag="OO161_001793"
     CDS             7613..10129
                     /gene="mrcB"
                     /locus_tag="OO161_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5462892.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLPA
                     AVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFP
                     DSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSG
                     FPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLVNLTAGRTVQGASTLTQQLVKNLFL
                     SSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFG
                     RPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDML
                     SARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEK
                     AAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGS
                     LAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDA
                     LTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQ
                     TIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGR
                     QLGVKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSI
                     YQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQ
                     QQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10349..12538
                     /gene="fhuA"
                     /locus_tag="OO161_001794"
     CDS             10349..12538
                     /gene="fhuA"
                     /locus_tag="OO161_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5462893.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGYYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12589..13386
                     /gene="fhuC"
                     /locus_tag="OO161_001795"
     CDS             12589..13386
                     /gene="fhuC"
                     /locus_tag="OO161_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5462894.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13386..14276
                     /gene="fhuD"
                     /locus_tag="OO161_001796"
     CDS             13386..14276
                     /gene="fhuD"
                     /locus_tag="OO161_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5462895.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14273..16255
                     /gene="fhuB"
                     /locus_tag="OO161_001797"
     CDS             14273..16255
                     /gene="fhuB"
                     /locus_tag="OO161_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5462896.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16290..17570)
                     /gene="hemL"
                     /locus_tag="OO161_001798"
     CDS             complement(16290..17570)
                     /gene="hemL"
                     /locus_tag="OO161_001798"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5462897.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVLPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17795..19216
                     /gene="clcA"
                     /locus_tag="OO161_001799"
     CDS             17795..19216
                     /gene="clcA"
                     /locus_tag="OO161_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5462898.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MELDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19240..19305
                     /locus_tag="OO161_001800"
     CDS             19240..19305
                     /locus_tag="OO161_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462899.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19298..19642
                     /gene="erpA"
                     /locus_tag="OO161_001801"
     CDS             19298..19642
                     /gene="erpA"
                     /locus_tag="OO161_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5462900.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19689..20312)
                     /locus_tag="OO161_001802"
     CDS             complement(19689..20312)
                     /locus_tag="OO161_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5462901.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVHQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20350..21149)
                     /gene="btuF"
                     /locus_tag="OO161_001803"
                     /pseudo
     CDS             complement(20350..21149)
                     /gene="btuF"
                     /locus_tag="OO161_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
     gene            complement(21142..21840)
                     /gene="mtnN"
                     /locus_tag="OO161_001804"
     CDS             complement(21142..21840)
                     /gene="mtnN"
                     /locus_tag="OO161_001804"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5462902.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDANV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     ILMVESLVQKLAHG"
     gene            21924..>22667
                     /gene="dgt"
                     /locus_tag="OO161_001805"
     CDS             21924..>22667
                     /gene="dgt"
                     /locus_tag="OO161_001805"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5462903.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRK"
BASE COUNT         5148 a         5740 c         6324 g         5455 t
ORIGIN      
        1 gatgggattg atatttatct gcgggaattt atattatgtt tctgcaaaaa tgtttaaact
       61 aaaattttta ttaaggaaag agaggatgta tgattttcag aaaatattta tattcgtaat
      121 ataaataaat taccatttgc ttaattcgtc aaatgctctt gtatgtaaga cttcacgcaa
      181 cgtctcccca tcaggaaacg ccagctccgg tgcaatctca aacagcggcc acagcataaa
      241 tccacgattc ttcatatcgt agtgcggaac ggtcaggcgt tcggtattta tcacttcatt
      301 accaaacagc atgatatcga gatccagcgt gcgtggtccc cagcgttcag ctttacgaac
      361 acgatcctgt tgcagttcta tgcgctgtgt atggttgagt aactcttccg gggcgagcga
      421 agtttcaagc gccacggcgg cgttgaggta atcgggttga tcttgcggcc ccagcggtgg
      481 agtgcggtaa aacgaagaaa cggcaagaat gtggctttca gggatatcgc ctaatgcttt
      541 cagggcagca ttgacctgct ccagcggaga ggccagattg ctgcctatgg caatatacgc
      601 cactgtcatg cggtaccctc acgacgcggt gcgcgtttgc gtggacgacg agtacggcga
      661 cgcgttgacg gctcttcatc caactcgtta agcatccctt tttgatctgg tggcgcggaa
      721 acctggaact caccccacca tttcaccaga cgctgcagtt cagcgttacg ttcaacttca
      781 gctcgcaagg ccaacaggtc ataagccgca cggaacttag gatgctccag cagtttccat
      841 gcgcgtttac cctgacgacg ggacatacgc aactgcaact gccagatatc gcgggttaac
      901 gtcgtcagac gtttcgggat tgccagtgaa cggcaggctt cgtccagcac gtcgttcatc
      961 gccagcgcga aagcgtcgtg ataggtcagg ccgctttctt gggcgatctt ctgtgccgtc
     1021 tccagcagtg ggtaccagaa catggcggca aacaagaacg ccgggttcac gcgcatatcg
     1081 ttatggatac gcgtatcggt attcttcagc acctgttcaa tgatccgctc catcgggctg
     1141 tcgccatttt ccgtgaagta gcgggtaatg gtcgggaaca gcggctggaa cagatgatat
     1201 tcacacaaca gcttataggt ttcgtaaccg tagcccgctt gtagcagttt aagcgattct
     1261 tcaaacaggc gtgccggtgg gatatcgttc agcagggtag cgaggcgagg gatcggttct
     1321 gcggtttccg ggctgatgcg catacccaat ttggcggcaa aacgtaccgc gcgcagcata
     1381 cgtaccggat cttcacggta gcgcgtttcc gggttaccaa tcagacggat aacgccgtcc
     1441 ttcagatcct tcatgccgcc aacgtaatca cggacggtaa aatccgctac gctgtaatac
     1501 aggctgttga tagtgaaatc gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg
     1561 tcgcgcagca acatgccgtt ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct
     1621 tcgtggtgtc cacggaaggt tgcaacttcg ataatctccg ggccaaacat cacatgagcc
     1681 agacggaaac ggcgacccac aaggcggcag ttacggaaca gtttgcgcac ctgctcaggc
     1741 gtggcgttag tggttacgtc aaaatctttc ggctttttgc caagtaacag gtcgcgcacg
     1801 ccgccgccaa ccagccaggc ttcgtatccc gctttattga gcctgtacat taccttcagg
     1861 gcattttcac tgatatcttt gcgggaaata gcatgctgct cacgcgggat caccgtcacc
     1921 tgtggacggg cgactgcctg ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa
     1981 aaattagcga ctcgggtaaa aatagtacac ctcggtagtg tcaaacatca ttcaggacaa
     2041 aaaaatagcg gctaatcata gctcagcatg acgcatttga gaatgttgaa tttacaattg
     2101 ccgactcggg cacggcggtc agcgtccagt ttttgacggc tgactgaagg atttgctcga
     2161 cgctgaaatc ctgccagtgt gcttctgcct gctgccccag aaattgaagt gccgcgatta
     2221 gtaccgggcg tggatcgcct ttcggcaacg caggcgcatg attctgcttg gaaagtttag
     2281 cgccttgtgg attaagcgcc agcggcagat gaatgtaatc tggcactttc cagccaaaaa
     2341 gctggtacag cgagatttgc cttactgttg gttcaatcag atcagcccca cgcactattt
     2401 ctgtaacgcc ctggaaatga tcatcaacca caacagccag gttgtaggcg aacaacccat
     2461 cacggcgatg aatgataaaa tcttcccgtg ccagtttttc gtcggcgtga ataatgccgc
     2521 gcagctggtc agtaaattgc gtgaccggat gctgctggcg gatacgcact gcggcgttgt
     2581 ctggtccatg atgcaacacc cggcaatgac cgtcgtaaat accgacaatg ctttgaatac
     2641 gcgcacgcgt acaggtgcag taataactta gtccttgttc atgtaaccag gcgagtgctt
     2701 cacgataggc gtcgtgacgt tgcgattgcc agagaacatc gccgtcccag tgcagaccgt
     2761 aatgttccag ctggcgcagg atagtttctg cggcaccggg aacttcacga ggcgggtcga
     2821 tatcttctat gcgtaccagc cagcgacctt gccgggcgcg agcctgcaaa tagctgccga
     2881 gcgcggcgat cagagagcca aaatgaagct cgccggaagg agagggggcg aagcggccaa
     2941 tatactgtgt gtctgtcatc tctttgaaca aaaaataagg cgggagcatt tcccgcctgt
     3001 ggtaaacgtg atggaacggc tgtaattagc cagccatctg tttttcgcga atttcagcca
     3061 gcgttttgca gtcgatgcac agatcggctg tcgggcgcgc ttccagacgg cgaataccaa
     3121 tttcaacacc gcaggattcg cagtagccga aatcttcgtc ttccactttt ttcagcgtct
     3181 tctcgatctt tttgatcagc ttacgctcgc gatcgcggtt acgcagttcg aggctgaact
     3241 cttcttcctg ggctgcacgg tctaccgggt ccgggaagtt ggctgcttca tcctgcatat
     3301 gtgtaacggt gcgatcgact tcatccctga gttgattacg ccatgcttcc agaatacgac
     3361 ggaagtgcgc cagctgggct tcattcatat actcttcgcc cggcttctcc tgatatggtt
     3421 ccaccccagc gatggcgaga atactcaggg acgatgtttt acggttttgc ccttcttgca
     3481 tgttgcttct ccttaacacg cactatcgat ccccatgttc gggggaaaaa tgaggccgct
     3541 ataaatagca gatgcttttc cggatagcaa ttatctaaac gtaacacttg acaactgtgt
     3601 gaggaaaagc gtatttgcgc acgcgaccag aatgtaaatt aaccagttac ttactttact
     3661 acaatgtaac cggcagtgat tttttaagag ccatgccttc agcagaaatt tccgctttgt
     3721 aagccagaat ttctaccccc ctctgttgag cttctgacaa tagttgcgcg tatttctcat
     3781 cgatgtggcg cgcgggtgaa aaccgtgtaa tggctgaatg cagcacggcg aaaaagataa
     3841 ccgcacgctg gccttcagcc gctacgttca tcaactcccg aaggtgtttc tgacctcgtt
     3901 cagtgaccgc atcgggaaaa tatccctgtt cgttctccgc taacgtaacc gatttcactt
     3961 caatatagca gtctggacgc gaatccgcct gcaacataaa gtcaatacgg ctgcgttctg
     4021 cgccatattt tacttcgctt ttcagcgagc tatagcctga cagttctgaa attgattcat
     4081 taaggatagc ctctttcgtc aacctgttag cccaaagcgt attgacgcaa ataaatgcgc
     4141 cgctctggct ttgagttagt tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag
     4201 tcgaatacca gacggtatcg ccacgcgttg cacaaccggt catcgcaccc gtattcgggc
     4261 agtgtagcgt taattcgcga ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac
     4321 gctgaattag cgtcgcgcgc tgtagagggg gagagaattc cattgcgaca atccttgtta
     4381 ttgcattagc gcccagcgtt ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga
     4441 ggcgtaaagg gtgaactccg tcaccgcata agaccagtta aaacctggcg gcgggattgt
     4501 caccgcctcg ctggcgtcgc gcaataaggt aatatgtgga tgaaacggac gattgctttg
     4561 aaaacaaccg ctgcgggcag cctgtgaacg gagcatattc gccagctgga ttaagccgcg
     4621 tggcggctga cgcatcccta accacaccac acgcgaacgc agccattgtc cggcgtcatc
     4681 aagcgtgagt gtgaaaccag gttgacgaat ccgtccggct aaaagagaaa gcgccttctc
     4741 tttctcggcg ctcacttcgc ctaaaaatgc cagagtcaga tgcaaattat cggcggcgac
     4801 cggacgtccc gcctcaggtg ggaagtgtgt ggcgcgccag tggataatct gttcgcggat
     4861 ttctgcaggt aagtcgatag caaagaacag acgttgcggt tcagacatgt gaggcactcg
     4921 gttatgaatt accgcgatgc tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt
     4981 tgtgtcgtcg ttgcccgttg ctgccgtctt acctgaatta cttaccgctc tcgattgtgc
     5041 gccgcaggta ttattaagtg cgccgaccgg ggccgggaaa tcaacctggc tgccgctgca
     5101 actgctggcg catcccggca ttaacgggaa aattatcctg ctggagccgc gtcgtctggc
     5161 ggcgcgtaac gtcgcgcaac ggctggagga gctgcttaac gaaaagccag gcgataccgt
     5221 tggctaccgg atgcgtgcgc aaaactgcgt cgggccgaat acccgcctgg aagtagttac
     5281 cgaaggcgtg ctgacgcgga tgatccagcg tgacccggag ttgagcggtg ttggattggt
     5341 gatccttgat gaatatcatg agcgtagctt gcaggcggat ttggcgttgg cgctgttact
     5401 cgatgtgcaa caaggtctgc gtgatgacct taaactgcta attatgtcgg ctacgctgga
     5461 caacgaccgc ttgcagcaaa tgctgccaga agcgcccgtc gtcatctcag aagggcgctc
     5521 gtttccggtt gaacgccgtt atttaccgct gcccgcgcat cagcgttttg acgatgccgt
     5581 tgcggtagcc accgctgaaa tgctgcgtca ggaaagcgga tcattactgt tatttttacc
     5641 tggcgtcgga gaaattcagc gtgtgcagga acaactggct tcgcgcatcg gcagtgatgt
     5701 attgctctgc ccgctgtatg gcgcgttgtc gctgaacgat cagcgaaaag cgatcctccc
     5761 ggcaccgcaa gggatgcgca aagtggtgct ggcgaccaat attgctgaaa ccagtttaac
     5821 catcgaaggt attcgtctgg tggtggattg tgcccaggag cgtgtggcgc gttttgatcc
     5881 gcgcacgggg ctaacgcgac tgattactca acgcgttagc caggcatcca tgacgcagcg
     5941 tgccgggcgc gccgggcgtc tggagccggg tatctgcctg catctaatcg ccaaagaaca
     6001 agcagaacgc gctgccgcgc aaagtgaacc ggaaatctta caaagcgatc tttccggttt
     6061 gctgatggaa ttactgcaat ggggatgcag cgatccggcg cagatgagct ggctggatca
     6121 accgccaacg gtgaatctac tggccgcgaa acgcctgtta caaatgctgg gggcgctgga
     6181 aggtgaacgg cttagtgcgc aggggcaaaa aatggcagcg ctgggtaacg atccgcgttt
     6241 agcggcaatg ctggttagcg cgaagaacga cgacgaagct gctaccgcgg caaaaattgc
     6301 cgccattctc gaagagccgc cacggatggg caatagtgac ctgggcgtgg cgttttcgcg
     6361 caatcaacca gcctggcagc aacgtagtca gcaactgtta aaacgcttaa acgtacgtgg
     6421 cggtgaggca gacagttcgc ttatcgcgcc gctacttgcc ggggcgtttg ccgatcgcat
     6481 tgctcgtcgc cgtgggcaag atggacgcta tcaactggca aacggcatgg gagcgatgct
     6541 cgatgccgac gacgcgctaa gccgccacga atggttgatc gcaccgttat tattgcaggg
     6601 cagcgcctcg ccggatgcgc ggattttact ggcgctgccg gtcgatattg atgagttagt
     6661 acaacgctgc ccgcagctgg tacagcagtc tgacactgtg gagtgggatg acgcgcaagg
     6721 tacgctgaaa gcctggcgtc ggctgcaaat cggtctgctg acggtgaaag tgcagccgct
     6781 ggcgaaaccc tcggaagacg agttgcatca ggcgatgctt aatggcatcc gtgataaagg
     6841 tttaagcgtg ctcaactgga cggcggaagc ggaacagcta cgcttgcgtt tgttatgcgc
     6901 cgcaaagtgg ttgccggaat atgactggcc agcggttgat gatgaaagtt tattggcgac
     6961 gctggaaacg tggctgctgc cacatatgac tggcgtacat tcactacgcg gcctgaaatc
     7021 actcgacatt tatcaggcac tacgcggatt acttgattgg ggaatgcagc aacgtctgga
     7081 tagtgaattg cctgcgcatt acactgtgcc gacgggaagc cggatcgcca ttcgttatca
     7141 tgaagataac ccgcccgcgc tggcggtgag aatgcaagag atgtttggcg aggccaccaa
     7201 tccgacgatc gcccaggggc gcgtgccgct ggtgctggag ttgctttcac ctgcccaaag
     7261 gccattacaa atcacacgag atttgagcgc cttctggaaa ggagcgtacc gtgaggtgca
     7321 aaaagagatg aaagggcgtt atcccaaaca tgtctggccg gacgacccgg caaatactgc
     7381 accgacgcga cggacgaaaa agtattcgta aggttggttt tctccctctc cctgtgggag
     7441 agggccgggg tgagggcatc agcgcgcacg ttcaccctca ccctaaccct aaccctctcc
     7501 ctcaagggag aggggaccga tcgaacacaa attttgagag atatcttctt ctgtcttgta
     7561 acagaagaac agaaaatcgg gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg
     7621 gaatgaccgc gagccaattg gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt
     7681 aagccgtcgt cgttacgaag atgacgatga ttacgacgat tatgatgact atgaggatga
     7741 agaaccgatg ccgcgcaaag gtaagggcaa agggcgtaag cctcgtggca aacgcggctg
     7801 gctatggcta ctgctaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt
     7861 ttatctcgat caaaaaattc gtagccgtat tgatggcaag gtctggcaac tgcctgcggc
     7921 agtttatggc cgaatggtca atcttgagcc agatatgacc atcagcaaga acgagatggt
     7981 gaagctgctg gaggcgactc agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt
     8041 taccgtgcag gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagtaa
     8101 agaagggcag gtgcgtgcgc gtctgacctt tgatggtgat catctggcga ccatcgtcaa
     8161 tatggagaac aaccgtcagt tcggcttttt ccgtcttgat ccgcgtctga tcaccatgat
     8221 ctcctcgcca aacggtgagc aacgtctgtt tgtgccgcgc agtggtttcc ctgatttgct
     8281 ggtggatact ttgctggcga cagaagaccg ccatttctac gagcatgatg ggatcagcct
     8341 ctactcaatt gggcgtgcgg tgctggtaaa cctgaccgca ggtcgcacgg tgcagggtgc
     8401 gagtacgctg acgcaacagc tggtgaaaaa cctgttcctc tccagcgagc gctcttactg
     8461 gcgtaaagcg aacgaagcct acatggcgct gatcatggac gcgcgttaca gcaaagaccg
     8521 tattcttgag ctgtatatga acgaggtgta tctcggtcag agcggcgaca acgaaatccg
     8581 cggcttcccg ctggcaagct tgtattactt tggtcgcccg gtagaagagc taagcctcga
     8641 ccagcaggcg ctgttagtcg gtatggtgaa aggggcgtcc atctacaacc cgtggcgtaa
     8701 cccgaaactg gcgctggagc gacgtaatct ggtgctgcgt ctgctgcaac agcaacagat
     8761 tattgatcaa gaactctatg acatgttgag tgcccgtccg ctgggggttc agccgcgcgg
     8821 tggggtgatc tctcctcagc cagcctttat gcaactggtg cgtcaggagc tgcaggcaaa
     8881 actgggcgat aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt
     8941 ggcccaggac gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg
     9001 taagttgagc gatcttgaaa ctgcgattgt ggtcgtcgac cgctttagtg gtgaagttcg
     9061 tgcgatggtg ggaggttctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg
     9121 tcgttcgatt ggttcccttg caaaaccagc gacttatctg acggctttaa gccagccgaa
     9181 aatctatcgc ctgaacacgt ggattgccga tgcgccaatc gcgctgcgtc agccgaatgg
     9241 ccaggtctgg tcaccgcaga acgatgaccg tcgttatagc gaaagcggca gagtgatgct
     9301 ggtggatgcg ttgacccgtt cgatgaacgt gccgacggta aatctgggga tggcgctggg
     9361 gctgcctgca gttacggata cctggatcaa actgggcgta ccgaaagatc agctgcatcc
     9421 ggttccggcg atgctgctgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcgtt
     9481 ccagaccatc gccagcggtg gtaaccgtgc accgctttct gcactgcgtt cggtaatcgc
     9541 ggaagatggc aaggtgctgt atcagagctt cccgcaggca gaacgcgctg ttccggcgca
     9601 ggcggcgtat ctgacactat ggaccatgca gcaggtggta caacgtggta cgggtcgtca
     9661 gcttggggtg aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa
     9721 cgtagatact tggtttgcag gcattgacgg cagcacggtg accatcacct gggtaggtcg
     9781 tgataacaac cagccgacca aactgtatgg agccagcggg gcaatgtcga tttatcagcg
     9841 ttatctggct aaccagacgc caacgccgct gaatcttgtt ccgccagaag atattgcaga
     9901 tatgggcgtg gactacgacg gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt
     9961 ctggaccagc gatccgcaat cgctgtgcca gcagagcgag atgcagcagc agccgtcagg
    10021 caatccgttt gatcagtctt ctcagccgca acagcaacct gctcagcaag agcagaaaga
    10081 cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac atctaagcgt
    10141 gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag atattaaccc
    10201 tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg taccgcttgc
    10261 gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc gtttacatta
    10321 tcattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc agccaaaaca
    10381 ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt ctgtttatgc
    10441 acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac ctgcgccgca
    10501 agaaagcgca tgggggcctg ctgcaactat tgcggcgaag cactctgcta ctgcgactaa
    10561 aacggataca ccaattgaaa aaacgccgca gtctatttcg gttgtgacaa atgaagagat
    10621 gcagatgcat caatttcagt ctgtaaaaga agcattaggt tatacacctg gcgttacggt
    10681 tagcagtcgt ggtgcttcta atacatatga ttttgttatc attcgtggtt tctcatctgt
    10741 tggtctgaat caaaataatt accttgatgg actaaaactt cagggcaact tttataacga
    10801 tgctgtgatt gatccttaca tgctcgagag ggttgaactg atgcgtgggc cgacgtctgt
    10861 tctctatgga aaaagtaatc ctggtgggat tatttctatg gtgagtaagc gtccgaccac
    10921 tgaacccctg aaagaaattc agtttaaaat ggggacggat aatctgtttc agaccggatt
    10981 cgatttcagt gatgcactgg atgataacgg tgagttctct taccgtttga cgggcctcgc
    11041 tcgctcaaca aacgaacagc agaaaaactc tgaatctcag cgttatacca ttgctccatc
    11101 attctcatgg cgtccagacg acaaaaccaa ttttactttc ctgtcctatt tccagaatga
    11161 accagaaacg ggttattacg gttggttgcc gaaagagggg accgttgagc cattgcctaa
    11221 tggtaagcgt ctaccgactg acttcaatga aggtgcatcg aataatacat actcccgtaa
    11281 ccagaaaatg gtgggatata gttttgaaca tggttttaat gacaccttca ccgtgcgtca
    11341 gaatctgcgt ttcagtgaaa tgaaaacctc acagaaaagt gtttatggca cagggattgc
    11401 tggtgatggt cataccctaa accgcgggac agtagtggat aatgagcgtc tacaaaactt
    11461 tagcgttgat acccaacttg aaagtaagtt tgctacaggt gacgtcgagc atactttgct
    11521 gacaggggta gacttcatgc ggatgcgtaa tgatatcaat gccagctttg gatccgcacc
    11581 atccatcgat ctttataaca aatatcaccc tgaatacttt gcatttggta gcgcagagcc
    11641 ataccagatg aatgaaagca aacaaacagg tatttatgtt caggatcagg cggaatggaa
    11701 taaatgggta ttcactctgg ggggacgcta cgattggtct aagcaagcga ccactgttcg
    11761 tgaaaactct tatacgccga ctgaaggtta tattgagcgc aatgatcatc agttcacctg
    11821 gcgcggtggt gtaaattact tattcgataa tggtatttca ccttacttta gctatagcca
    11881 gtcctttgaa ccgagtgctt tcgatctgtg gagcaaccca cgcatttcct ataagccatc
    11941 gaaaggtgaa cagtatgaag ctggcgtaaa atatgttccg aatgatatgc cggtcgttgt
    12001 tacgggcgca gtctatcaat tgacgaaaac aaataacctg acagcagacc caacaaaccc
    12061 attagcgcaa gtcccagcag gtgagattcg cgctcgtggt gtggaacttg aagcaaaagc
    12121 ggcgttaaat gccaatatta acttgacggc ttcttatacc tacaccgatg cggaatacac
    12181 caaagacacc aatctcaaag gtaaaactcc agaacaagta ccggagcata tggcatctct
    12241 ctgggggtat tataccttca atgaagggcc gctttctggt ttaacattgg gaacaggtgg
    12301 tcgttttatt ggttccagct atggtgatcc ggcaaacact tttaaagtgg gtagcgcagc
    12361 tgtaatggat gctgttgtaa aatatgatct ggcacgcttt ggtatggcgg gatccagcct
    12421 tgctgtgaac gtcaacaatt tgctcgatcg tgagtatgtt gccagttgct tccagactta
    12481 tggctgcttc tggggcgcag aacgtcaggt cgttgcaacc gcaaccttcc gtttctaatt
    12541 tctcttttgg ggcacggatt tccgtgccca tttcacaagt tggctgttat gcaggaatac
    12601 acgaatcatt ccgataccac ttttgcactg cgtaatatct cctttcgtgt gcccgggcgc
    12661 acgcttttgc atccgctgtc gttaaccttt cctgccggga aagtgaccgg tctgattggt
    12721 cacaacggtt ctggtaaatc cactctgctc aaaatgcttg gccgtcatca gcctccgtca
    12781 gaaggggaga ttcttcttga tgcccagccg ctggaaagct ggagcagcaa agcgtttgcc
    12841 cgcaaagtgg cttatttgcc gcagcagctt cctccggcag aagggatgac cgtgcgtgaa
    12901 ctggtggcga ttggtcgtta cccgtggcat ggcgcgctgg ggcgctttgg ggcggcagat
    12961 cgcgaaaaag tcgaggaagc tatctcgctg gttggcttaa aaccgctggc gcatcggctg
    13021 gttgatagtc tctctggcgg cgaacgtcag cgggcgtgga tcgccatgct ggtggcgcag
    13081 gatagccgtt gtctgttgct cgacgaaccg acctcggcgc tggatatcgc ccaccaggtt
    13141 gatgtgctgg cgctggtgca ccgtttaagt caggagcgtg gcctgacggt cattgccgtg
    13201 ttgcacgata tcaatatggc ggcacgctac tgtgattatc tggtcgccct gcgcggtggt
    13261 gaaatgattg ctcagggaac gcctgcggaa attatgcgcg gcgataccct cgaaatgatt
    13321 tatggcatcc cgatgggtat tttgccgcat ccggcgggtg ctgcacctgt gagttttgtt
    13381 tattgatgag cggcttacct cttatttcgc gccgtcgact gttaacggcg atggcgcttt
    13441 ctccgttgtt atggcagatg aataccgccc acgcggcggc tattgatccc aatcgtattg
    13501 tggcgctgga gtggttgccg gtggaattac tgctggcgct cggcatcgtg ccttacggcg
    13561 tggcggatac catcaactat cgcctgtggg tcagcgaacc accattgccg gactcagtga
    13621 tcgacgtcgg tttgcgcaca gaacctaacc ttgaactgct gaccgaaatg aaaccgtcgt
    13681 ttatggtctg gtcggcagga tatggccctt cagcagaaat gctggcacgt attgcgccgg
    13741 ggcgtggatt taacttcagc gacggcaaac agccgctggc gatggcgcgt aaatcactga
    13801 cggaaatggc agatttactt aacctgcaaa gcgcagcaga aacgcattta gcgcaatatg
    13861 aagactttat ccgcagcatg aaaccccgct ttgtgaagcg tggtgcacgc ccgttattgc
    13921 tgacgacgct tatcgatccg cgccatatgt tggtcttcgg tccaaacagc ttgttccagg
    13981 aaattcttga tgagtacggc atcccaaatg cctggcaagg ggaaaccaac ttctggggca
    14041 gtaccgccgt cagtatcgat cgtctggcag cgtataaaga cgttgatgtg ctctgttttg
    14101 atcacgacaa cagcaaagac atggatgcgc taatggcaac cccgctgtgg caggccatgc
    14161 cgtttgtccg cgccggacgc tttcagcgcg tacccgcagt ctggttttat ggtgcgacgc
    14221 tctcggcaat gcactttgtg cgcgttctgg ataacgccat cggaggtaaa gcgtgagtaa
    14281 acgaattgcg cttttcccgg cgttattgct ggcgctgctg gtgattgtcg ctacggcgct
    14341 gacctggatg aacttttcgc aggcgctgcc gcgtagccag tgggcgcagg ccgcctggtc
    14401 gccggatatt gacgtcatcg agcagatgat ttttcactac agcttgctgc cgcgtctggc
    14461 gatttcgctg ctggtgggcg caggcctggg gctggtgggc gtgctgtttc agcaagtgct
    14521 gcgtaatccg ctggcggaac cgacgacact tggcgttgct acaggcgcgc aactggggat
    14581 taccgtcact acgctctggg cgatcccagg ggcgatggcg agtcagtttg ctgcgctggc
    14641 gggcgcttgc gttgttggct taatcgtctt tggcgtcgcg tgggggaaac ggctttcgcc
    14701 ggtaacgctg atcctcgcgg ggctggtagt gagcctttat tgtggggcaa tcaatcagtt
    14761 actggttatc ttccaccatg accaactgca aagcatgttc ctgtggagca ccggaacgct
    14821 gacgcaaacc gactggggcg gtgttgagcg tttatggccg cagttgctgg gcggtgtgat
    14881 gctgacgttg ctgctacttc gcccgttaac cctgatgggg cttgatgatg gcgtggcgcg
    14941 caatctcggg ctggccttgt cgctcgcgcg tctggcggcg ctgtcgctgg cgattgtcat
    15001 cagtgcgctg ctggtgaacg cggtggggat tatcggcttt atcgggttgt ttgcgccgtt
    15061 gctggcaaaa atgctggggg cgcggcgtct gctgccgcga ctgatgctgg cgtcgttgat
    15121 tggtgcgctg atcctctggc tttccgatca aatcatcctc tggctgactc gcgtgtggat
    15181 ggaagtgtcc accggttcgg tcactgcgtt gatcggtgcg ccgctgctac tgtggctgtt
    15241 gccgcgttta cgcagcatta gcgcgccgga tatgaaggtc aacgatcgtg tcgcggctga
    15301 acgccaacat gtgctggcgt ttgccctcgc gggcggcgtg ctgctgttga tggctgtggt
    15361 ggtggcgctg tcgtttggtc gtgatgcgca cggctggatg tgggcgagcg gggcgttgct
    15421 cgatgattta atgccctggc gctggccgcg aattatggcg gcgctatttg cgggcgtcat
    15481 gctggcggtg gcgggctgta ttattcagcg actgaccggc aacccgatgg caagcccgga
    15541 agtgctgggg attagctccg gcgcagcgtt tggcgtggta ctgatgctgt ttctggtacc
    15601 gggtaatgct tttggctggc tgttacccgc gggaagtatc ggtgccgcgg tgacgctgtt
    15661 gattattatg atcgccgccg ggcgcggtgg attttcccca caccgcatgt tactggcggg
    15721 gatggcgtta agtaccgcat tcaccatgct tttaatgatg ttgcaggcga gtggtgaccc
    15781 gcgaatggcg caagtgctca cctggatctc tggttcgacc tacaacgcca ccgatgcgca
    15841 ggtctggcgt accggaattg tgatggtgat tttgctggcg attacaccgc tgtaccgccg
    15901 ctggctgacc attttacctc tgggcggtga taccgcacga gctgtgggaa tggcgctgac
    15961 gccgacgcga attgcgctgc tgctgttagc ggcttgcctg acggcgaccg cgacgatgac
    16021 tattggaccg ttgagttttg ttggtttaat ggcaccgcat attgcgcgga tgatgggttt
    16081 tcgacggacg atgccacaca tcgtaatttc ggcgctggtg ggtggtttac tgctggtatt
    16141 cgctgactgg tgtgggcgga tggtgctgtt tccattccag atcccggcgg ggctgctgtc
    16201 gacctttatc ggcgcgccat attttatcta tttgttgaga aagcagagcc gttaattgag
    16261 tgcctcgccc ggtgggcgag gcaaaattat cacaacttcg caaatacccg acgtgcagca
    16321 tcgatggtgt tattgatatc ttccatgctg tgcgccacag acataaagcc cgcttcaaac
    16381 gctgacggtg ccaggtaaac accttcgtcc agcatcatat ggaagaaacg cttaaagcgt
    16441 tccacgtcac aggccatcac atcctgatag cacgtcacgg actcggcgtc ggtaaagaaa
    16501 ataccgaaca tgccgccaac gtggttaacg accagcggag ttccggcttc ttctgccgct
    16561 tccagcagac cttctgccag acgtgttgtc agctcatcca gcgtttcgtg aacgcccggc
    16621 tgcgcgactt cattcagaca ggcgaaaccc gctgccatcg caatcgggtt accggaaagc
    16681 gtacccgcct gatagaccgg acccgtcggg gccagcgcat ccattacatc acgacgacca
    16741 ccgaatgcgc ctaccggcat tccaccgccg atgattttgc cgaggcaggt taaatctggc
    16801 actacgccgt aataatcctg tgcgccagcc agtgccacgc ggaagccggt catcacttca
    16861 tcgatgatca gcaacgcgcc aaattcgtcg cacagcgcac gcagacctgg caggaactct
    16921 ggctgcggca gaacacagtt catattgcct gccaccggct cgacgataat acaggcaatc
    16981 tcttgcgggt attgctcaaa cgcggcgcgt acagaagcca gatcgttata agtacaggtt
    17041 aaggtatgtt tggcgaaatc tgccggaaca cccggcgagt ttggctggcc taacgtgagt
    17101 gcgccagaac cggctttcac cagcaggcag tcagcgtgac cgtggtaaca accttcaaat
    17161 ttaatgattt tgtcgcgacc ggtaaaacca cgggccaggc ggatggcgct catggtcgct
    17221 tcagtgccgg agttcaccat gcgcaccata tccatggtcg ggaccagttc ggtcaccagt
    17281 tgcgccattt tcacttccat ttcggtcggt gcaccaaagc ttaaaccacg ctcggcggct
    17341 tcaatcacgg cattgcggat agccggatgg ttatggccca gcaccatcgg cccccaggaa
    17401 ccgacataat cgatataggc tttgccatca acatcgtaca gataagcgcc gtccgctttt
    17461 tcgataaaca gtggagtgcc gcccacgcca gtaaaggcgc gaacagggga gttcacaccg
    17521 ccagggagca gctcgcgcgc tgcgctgtaa agattttcag acttactcat ggagggttcc
    17581 tgattcgtag aaaaagtgaa tggctgctat tctatgttat tcataacaag ttaaatactc
    17641 gtcaaacatc aggctgcttg tactggtgca atcctgattt cttagagtat aaaagttttg
    17701 tgcatttgaa acattacgct ttgcaaagga ttttcatgga acgtgcgagt aaaatgccgt
    17761 catcttattt gtatgaccaa taagtgatca ttggatgaaa actgatactc cctctttaga
    17821 aacaccgcag gccgcgcgcc tgcgacgcag acaactgatt cgccaacttc ttgagcgcga
    17881 taaaaccccg ttagccattt tgtttatggc ggcagtcgtc ggcacgcttg tcgggctggc
    17941 agcggttgct tttgacaaag gtgtcgcctg gttgcagaac caacgtatgg gggcgctggt
    18001 acatactgct gataattatc cgcttctgtt aaccgtcgct tttctctgtt cggcggtgct
    18061 ggcgatgttc ggctactttt tggtgcgcaa atacgcgccg gaagcaggtg gttcggggat
    18121 cccggaaatt gaaggggcgc tggaagacca acgtcccgtt cgctggtggc gtgtgttgcc
    18181 ggtgaaattc tttggcgggc tggggacact cggcggaggc atggtgctgg ggcgcgaagg
    18241 gccaaccgtg cagatcggcg gcaatatcgg tcgcatggaa ctcgatattt tccgcctgaa
    18301 aggtgatgaa gctcgccata cgctgctggc aaccggtgct gctgcggggc tggctgcggc
    18361 ctttaacgcg ccgctggcgg gtattttgtt tattatcgaa gagatgcgtc cgcagtttcg
    18421 ctatacgtta atttcgatta aagcggtatt tattggcgtc attatgtcga ccattatgta
    18481 ccgcattttt aatcatgaag ttgcgttgat tgacgtcggt aaactttctg atgcgccgct
    18541 taatacgctg tggctttatc tgatcctcgg tattattttt ggcattttcg gccctatttt
    18601 taataaatgg gtgctgggga tgcaggattt gctgcaccgt gtgcacggcg gcaatattac
    18661 caaatgggtg ctaatgggcg gtgcgattgg cggtctgtgt ggattgctgg ggtttgtggc
    18721 accagcaacg tcgggcggcg gttttaacct gattcctatc gctaccgcag ggaatttcag
    18781 catgggaatg ctggtgttta tcttcgtcgc gcgggtcatt accaccttac tgtgcttctc
    18841 ttccggcgcg ccgggcggta tttttgcccc gatgctggcg ctgggtactg tgctgggaac
    18901 cgctttcgga atggttgtcg ttgagctgtt tccgcaatat caccttgagg cggggacttt
    18961 tgctattgcc ggaatggggg cgttactggc ggcatctatt cgcgcgccgt taacggggat
    19021 cattttggtt ctggagatga ccgataacta ccagctcatt ttgccaatga ttattaccgg
    19081 tcttggcgca acactattag cgcaatttac cggcgggaaa ccgctatatt cggcgattct
    19141 tgcgcgcacg ctggcaaaac aggaagctga gcaactggcg cgaagcaagg ccgcatcagc
    19201 cagcgagaat acttgaacga aataccaggg tattagataa tggcgattat tattgggtta
    19261 gaatttgccc aattgccgat gtcgtttgga gcaaaatatg agtgatgacg tagcactgcc
    19321 gctggagttt accgacgcag cagccaacaa agttaaaagc ctgatcgctg acgaagataa
    19381 cccgaatctg aaattacgcg tgtatatcac cggtggcggt tgcagcggct tccagtatgg
    19441 tttcaccttt gatgatcagg tgaacgaagg cgatatgacc atcgaaaaac agggcgttgg
    19501 cctggtggtt gatccgatga gcctgcaata tctggtcggt ggttccgttg actataccga
    19561 aggactggaa ggttctcgtt tcatcgtgac caacccgaac gcgaaaagca cctgcggttg
    19621 cggttcttcc tttagcatct aatctgttgt tcatgtgccg gataaactat ccggcacatc
    19681 ttctctcttc aacgcccatt ctcatccagc gcaaacgtcg gtagcttaag atgccaacga
    19741 atagccgcca gccgaatcaa tagcgtcacg accatgccca tcatactggc tgtttccagt
    19801 ggtacggaaa atgtgtaata agccgtagcg tggacaatac cgccgataat acaggcagtt
    19861 gcgtagattt ctgtacgtaa aatcatgggg atttcgcggg ccagtacatc acgaatgatc
    19921 ccgccgccaa cgccagtaat gacgcccata caaaccgcga ttaacggacc ggcttccgca
    19981 ttaaaggctt tattcacgcc aatgccgaca aacaccgcca gaccaacggc gtccagcacc
    20041 ggcaacatcc attttggtaa gcgtcttggc tggtgcacca gcacgatggt cagcatgctg
    20101 gttaccattg caacgaccag atcggtggga tctttcaccc aaaataccgg gccgtgatcc
    20161 agcgccatgt cgcgaattgt cccgccgcct acagcggtaa ccacgcccag taccagaaca
    20221 ccaaaagggt ccatacgcaa ttttccggct aacaaaacgc cggagatggc aaataccgct
    20281 gtgccgacta tatccagcca atagacgagc attgttcaat ccccactgag cacctaaaaa
    20341 ggtcaggcgc taatctacct gtgaaagcgc attacagagc tgttgtgcag cgaggataat
    20401 acgtgggctt gcacgtcaaa ccagtcactc gtgagaggaa taacgggaat tttgagctgt
    20461 tcaccccagt attgtttgat tttaggaatt tggtccggtc cgcctgtaat gacaatcgcc
    20521 tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg gccaaggaac ccggctgtct
    20581 ttaaagatgt tttctccgcc acaaacttcg agtacctggt tttgaatcga ctcttttcca
    20641 ctggtaaatg gcggattaat gccgaattgc agaaaaacac gttttttagg tttatcagca
    20701 tattgcgctt tcaattgcgc gtactgatcc agcagggatt gcgcggcttg ttcggcctta
    20761 tccggttgcg gactccaggg ggccagttga cgtaacgcat tggcaatttg ttcaatgctt
    20821 gtcgcatcga cccacatcac ttttattccc agcgaagcca gctggtcaac ctgccgctcg
    20881 gcattacctc cacgccaggc aatcaccaga tcgggtttca gcgcgacaat gcgttccaga
    20941 ttcatcccct gccaggtgga aacctgctca atcttttgcg cttgtggagg atagtcggaa
    21001 tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg caagttcagt gttggcggga
    21061 gaaagcgtga tgacgcgcgg tgccgcgttg agccacagtg gcgcgagaaa agacagggcg
    21121 accagcgccc tgaacagtga cttagccatg tgccagtttc tgcaccagtg actcaaccat
    21181 caggatggac tgtttagcgg caacagccag gaactcatcg aagctaagat gagactgttg
    21241 atcggccacg tcggagatgg cgcgtacgac aacaaacggg acgttgaaat tgtggcagac
    21301 atgggcgatt gccgtcgctt ccatctctac agcaatggcc tgtgggaagt tgtggcggat
    21361 tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg ctaacaatca ggccacgtac
    21421 agcgttaaga ttcagttcgg caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc
    21481 tttaaagcct gccggacagc ctggtaactg accgtattca taaccaaatg ccgtgacatt
    21541 cgcgtcgtga taacgtgctt cgtccgagac aacgatatcg cccactttca acgttggtgc
    21601 caggccaccg gcagaaccgg tgttaataat cacatctggc ttgcagtgtt ccaacagcaa
    21661 agtggcaccc agcgccgcag cgactttacc gatgcccgat ttcagaagcg caacctcggt
    21721 tccattcagt tggccggtat agatttcgca accgccgaga ctgatagttt gacgattttc
    21781 gattttgtca cgcagcagcg taacttcttc ttccattgca ccaatgatgc cgattttcat
    21841 agatttactc gcgataagcc cgatttgaag gcatagttta ccatgcgctt acggggaagc
    21901 gtatttctca cgcgggagag gacatggcac agattgattt ccgaaaaaaa ataaactggc
    21961 atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca tgagatcctg cggatcttcg
    22021 agagcgatcg cgggcgtatc atcaactctc cggcaattcg tcgtctgcaa caaaagaccc
    22081 aggtttttcc actggagcgc aatgccgccg tgcgcacgcg tcttacccac tcgatggaag
    22141 tccagcaggt ggggcgctac atcgccaaag aaattctaag ccgtatgaaa gagcttaaat
    22201 tactggaagc atacggcctg gatgaactga ccggcccctt tgaaagcatt gttgagatgt
    22261 catgcctgat gcacgatatc ggcaatccgc cgtttggtca ttttggcgaa gcggcgataa
    22321 atgattggtt tcgccagcgt ttgcacccgg aagatgccga aagccagcct ctgactgacg
    22381 atcgctgcag cgtggcggca ctacgtttac gggacgggga agaaccgctt aacgagctgc
    22441 ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa tgcacaaggc attcgtctgg
    22501 tgcatacatt gatgaggatg aatctcacct gggcacaggt tggcggtatt ttaaaatata
    22561 cccgtccggc gtggtggcgt ggcgaaacgc ctgagacaca tcactattta atgaaaaagc
    22621 cgggttatta tctttctgaa gaagcctata ttgcccggtt gcgtaaa
//
LOCUS       DAJTNA010000057        22359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTNA010000057 DAJTNA010000000
VERSION     DAJTNA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22359
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.058"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..1298
                     /locus_tag="OO161_001806"
     CDS             <1..1298
                     /locus_tag="OO161_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5462904.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRLLTQDEIDVRIKRFWRYLDRTLPGAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEITPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCPQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLPDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
     gene            1270..2133
                     /locus_tag="OO161_001807"
                     /pseudo
     CDS             1270..2133
                     /locus_tag="OO161_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
     gene            complement(2347..3129)
                     /locus_tag="OO161_001808"
     CDS             complement(2347..3129)
                     /locus_tag="OO161_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5462905.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRVAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(3126..4199)
                     /locus_tag="OO161_001809"
     CDS             complement(3126..4199)
                     /locus_tag="OO161_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5462906.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGNLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(4321..4482)
                     /locus_tag="OO161_001810"
     CDS             complement(4321..4482)
                     /locus_tag="OO161_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5462907.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OO161_001811"
     CDS             complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OO161_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5462908.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTEQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OO161_001812"
     CDS             complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OO161_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5462909.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(7269..7964)
                     /gene="yjjG"
                     /locus_tag="OO161_001813"
     CDS             complement(7269..7964)
                     /gene="yjjG"
                     /locus_tag="OO161_001813"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5462910.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKHRLPPPR"
     gene            complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OO161_001814"
     CDS             complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OO161_001814"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5462911.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OO161_001815"
     CDS             complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OO161_001815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5462912.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            8910..9941
                     /gene="rsmC"
                     /locus_tag="OO161_001816"
     CDS             8910..9941
                     /gene="rsmC"
                     /locus_tag="OO161_001816"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5462913.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLNAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            10209..10295
                     /locus_tag="OO161_001817"
     tRNA            10209..10295
                     /locus_tag="OO161_001817"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10243..10245,aa:Leu,seq:cag)
     gene            10324..10410
                     /locus_tag="OO161_001818"
     tRNA            10324..10410
                     /locus_tag="OO161_001818"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10358..10360,aa:Leu,seq:cag)
     gene            10445..10531
                     /locus_tag="OO161_001819"
     tRNA            10445..10531
                     /locus_tag="OO161_001819"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10479..10481,aa:Leu,seq:cag)
     gene            complement(10570..10806)
                     /locus_tag="OO161_001820"
     CDS             complement(10570..10806)
                     /locus_tag="OO161_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5462914.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            10947..11735
                     /gene="fhuF"
                     /locus_tag="OO161_001821"
     CDS             10947..11735
                     /gene="fhuF"
                     /locus_tag="OO161_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5462915.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(11773..12449)
                     /gene="bglJ"
                     /locus_tag="OO161_001822"
                     /pseudo
     CDS             complement(11773..12449)
                     /gene="bglJ"
                     /locus_tag="OO161_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(12407..13132)
                     /locus_tag="OO161_001823"
     CDS             complement(12407..13132)
                     /locus_tag="OO161_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5462916.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            13751..14521
                     /locus_tag="OO161_001824"
     CDS             13751..14521
                     /locus_tag="OO161_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5462917.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            14512..14985
                     /locus_tag="OO161_001825"
     CDS             14512..14985
                     /locus_tag="OO161_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5462918.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            15093..15632
                     /gene="dnaT"
                     /locus_tag="OO161_001826"
     CDS             15093..15632
                     /gene="dnaT"
                     /locus_tag="OO161_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5462919.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            15635..16372
                     /gene="dnaC"
                     /locus_tag="OO161_001827"
     CDS             15635..16372
                     /gene="dnaC"
                     /locus_tag="OO161_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5462920.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            16421..16915
                     /locus_tag="OO161_001828"
     CDS             16421..16915
                     /locus_tag="OO161_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5462921.1"
                     /translation="MMKTVKHLLCCAIAASTLIPTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDINYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            17169..19460
                     /gene="opgB"
                     /locus_tag="OO161_001829"
     CDS             17169..19460
                     /gene="opgB"
                     /locus_tag="OO161_001829"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5462922.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(19599..20621)
                     /gene="lgoD"
                     /locus_tag="OO161_001830"
     CDS             complement(19599..20621)
                     /gene="lgoD"
                     /locus_tag="OO161_001830"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR5462923.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            20760..21674
                     /locus_tag="OO161_001831"
     CDS             20760..21674
                     /locus_tag="OO161_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5462924.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLESISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            21889..>22359
                     /locus_tag="OO161_001832"
     CDS             21889..>22359
                     /locus_tag="OO161_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5462925.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVIND"
BASE COUNT         5516 a         5846 c         5536 g         5461 t
ORIGIN      
        1 aatggctgga gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc
       61 tttaccacca cgtaccgtcg gtcacatcga tgccggtcta cctggggcaa ctggatgcgt
      121 tgttgcaacc gtatgttaga cttctaacac aagacgagat cgatgttcga ataaaacgtt
      181 tctggcgtta cctcgacaga accctgccag gcgcctttat gcacgccaat atcggcccgt
      241 ctgattcgcc cattacccgc gcgatcttac gtgcagacgc agagttgaag caggtttcac
      301 cgaacctgac ctttatctac gatcctgaaa tcacccctga tgacctgctg ctggaagtgg
      361 cgaagaacat ctgtgaatgt agcaaaccgc acatcgccaa cggtccggtg catgataaaa
      421 ttttcacaaa agggggctac gggattgtga gctgttacaa ctcactgccg ctggcgggtg
      481 gtggcagcac gctggtacgc cttaacctga aagccattgc cgagcgcagc gaatcgctgg
      541 atgacttctt tacgcgcact ctaccgcact actgcccgca gcagatcgcc atcatcgatg
      601 cgcggtgtga attcctctat caacaatcac acttctttga gaatagcttc ctggtgaaag
      661 aagggctgat taaccctgaa cgttttgtgc caatgtttgg catgtatggg ctggcggaag
      721 cggttaactt gctgtgtgag aaagaaggga ttgccgcgcg ctacggtaaa gaagccgccg
      781 caaatgaagt gggttatcgc atcagcgcgc aactggcgga gtttgtcgcc aatacccccg
      841 tgaaatatgg ctggcaaaaa cgcgccatgt tacacgcaca gtcggggatc agttccgata
      901 tcggcaccac gccgggcgcg cgtttgccgt atggcgatga gccagatccg atcatccatc
      961 tgcaaactgt cgccccgcat catgcttatt attattccgg catcagcgac attctgacgc
     1021 tcgacgaaac catcaaacgt aatccgcagg cgctggtaca gctttgcctc ggtgccttta
     1081 aagccggaat gcgtgaattt accgccaatg tcagcggtaa cgatctggtt cgcgttaccg
     1141 gttatatggt gcgtttgccg gatttagaaa aatatcgcgc cgaaggttca cgcaccaaca
     1201 ccacctggct gggcgaagaa gccacacgca acactcgtat tctggaacgc cagccgcgcg
     1261 tgataagcca tgaacagcag atgcgcttta gtcagtaaga ttatcccctt ctcctgcgtt
     1321 gacgggccag gcagtcgtct ggctctgttt ctgcagggct gcaatctgcg ctgaaaaaac
     1381 tgtcacaatc cgtggacgat ggaacgctgc aatgattgcg gagagtgcgt gccacagtgt
     1441 ccgcatcagg cgttgcagat tgttgacggc aaagtgctgt ggaacgctgt ggtctgcgag
     1501 cagtgtgata cctgcctgaa gatgtgtccg caacatgcca cgccaatggc gcaatccatg
     1561 agcgtggacg aagtgcttag ccatgtccgc aaagcggtgc tgtttattga agggataacg
     1621 gtaagcggcg gcgaagccac gacccaactg ccgtttgtag tggcgctgtt tactgctatc
     1681 aaaaacgatc cgcaactgcg ccacctcacc tgcctggtgg acagtaacgg catgttgagc
     1741 gaaaccggct gggaaaaatt actcccggtg tgtgacggcg caatgctcga tctcaaagcg
     1801 tgggggagcg aatgtcatca acacttaacc ggacgcgata atcagcagat taagcgcagc
     1861 atctgtttgc ttgcagagcg cggcaagctg gcagaactgc gtttgctggt aattccagac
     1921 caggtggatt atttgcatca catcgatgag ctggcgacgt ttatcaagag acttggcgat
     1981 gttccggttc gcctgaatgc gtttcatgcc cacggcgtgt atggcgaggc gcaaagctgg
     2041 gcgagcgcca cgccggaaga cgttgagccg ttggctgatg cgttaaaggt gcgcggggtg
     2101 agccggttga tatttccggc gctctatttg tgacatgccg gatacggcct acggttcggc
     2161 atagacttgt aggcatgata agacgcgaca agcgtcgcat caggcattgt gcgccaactg
     2221 ccggatgcgg cgtgaacgcc ttatccggcc tatggttcgg cacaaacctg taggcatgat
     2281 aagacgcgac aagcgtcgca tcaggcatta tgcgccgact gccggatgct gcctacgaag
     2341 ggtaaactac ccactaacgc taaacaacgc atacgtatta ttaagcaaca cttcggcaat
     2401 ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgca cagcctgttc
     2461 cggtcggtta ggctgcccct gaaagccgtt gagcggcata tccggcgcgt cggtttccag
     2521 caacaacgat gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata
     2581 ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg
     2641 caggctgccg gaaaaaccgt gaaccacgcc agtgcgcgat aaatcgtgtc gtttaagatg
     2701 catcgccagt ttgtcgtgtg tgcgccgtga atgcaggatc accggcagat cgtagcgttt
     2761 cgccagtttc agctgttcgt cgagtaacca ctgctgcctc tcaagttgcg gatcgtcgcc
     2821 gaagagatcc agaccgacct cccccactgc caccaccttc gccggacgcc tttccagcgc
     2881 ctgctgtagc tgctcaagag acacatcgct atgtttttcc aacatacctg gatgcaagcc
     2941 caatgcggca tacagcggtt gatatttttc cgctaacgcc tgcacacggg caaaattttc
     3001 cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct tgtgccacgc gttgcaggct
     3061 ggcctcttca tcgccactaa acggcgggaa atcaaaatgg cagtgggtgt cgataaaacg
     3121 gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcgtcaatc
     3181 acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgattccggc
     3241 gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc
     3301 gtggcgagga aataacgacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga
     3361 atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc
     3421 agattgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc
     3481 tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg
     3541 aaccactgcg gcgtgtagta catttgtgac ggcacagtgc gaatgacgac caacgtttta
     3601 gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag
     3661 ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga
     3721 atcacatcca gccagttttg tttggttggt aaaaagtaat taggcgcgta gtcatcctga
     3781 cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt
     3841 tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct
     3901 ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact
     3961 ttgcgcgcgt aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat
     4021 gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca
     4081 gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg
     4141 aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc
     4201 tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa
     4261 aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat
     4321 ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa
     4381 gacaatttta gctgcgcctg cagcggtacc ggccaaacca ccaaacccaa gtgcggcggc
     4441 gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat
     4501 agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg
     4561 tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt
     4621 cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc
     4681 actttgtgcc tgagaatcta cggtaccgga gagctgaacc acgccgtcgg tggtttcaac
     4741 tttcacatga cgggaaggga cgatatcgtc cgccagcagt ttggctttga tttcactggt
     4801 ggtggcggtg tcacccgcgt agcccttcac cgagccttct ttagcgtcgc gaacgtgcag
     4861 tttgtcgctg acagaggtca ccccttcaac gcctttcgcc actttcactg cctcttcggc
     4921 ctgagcctgg ctttcaacga aaccgctcag ggtcacgact ttttgctcgg ttttgacaga
     4981 gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat
     5041 ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc
     5101 gctttcattg gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga
     5161 ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt
     5221 attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg
     5281 gagagacttc accctctaca gaagtcaatg gtcgccatca caaaagcgat gggtgatgaa
     5341 taacgaccat tacagcctct aaatcagtta ttaatatcgg tagaatgaca atcgacggct
     5401 ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt
     5461 gttaaatata gatcacaatt ttgaaaccgc tcgtgatatc agcgaaaaca taagcaaaag
     5521 tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag
     5581 cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg
     5641 ggataacgtt cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta
     5701 tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc
     5761 gcgtccgcac attctaccca gcgggcagtg gcaacgttga ctgattcata tactgcttca
     5821 acgttgtatt cgctcttcag gcgcgatacc accacatcaa actgcagcac accaacagca
     5881 ccaacgatca aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa
     5941 agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga
     6001 cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta
     6061 aaggtgtcgc cgatctgaat agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac
     6121 gcttcttcaa cgtgcgaacg gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca
     6181 tctttcgcag tacgcacctg gcgcagcttc atgccttttt catatttacc ggatactacg
     6241 cgcataaacg ccacgcggtc gcgatgtttc gggtccatat tggcctgaat tttaaatacg
     6301 aagccggtaa atttgtcttc gctcgcctct acggtacggg tatcagtctg acgcggcatc
     6361 ggcgcagggg cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc
     6421 gcagtaccga agaataccgg agtgatttcg cccgcaagga acagttcttt gtcgaactcg
     6481 ttagacgcgc ctttcaccag ttccagttcg tcacgtagct gctgtgccag atcttcacca
     6541 accgcagcgt cgagatccgg gttattcagc cctttaacaa tacggacttc ctgaatcgtg
     6601 tggcctttac cgctctgata aagataggtt tcgtctttat aaaggtggta aacgccttta
     6661 aacagcttgc cgcagccaat cggccaggtg atcggtgcgc agccgatttt cagctcgttc
     6721 tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag
     6781 gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca
     6841 acaccttttg cagcgtcgat aaccatcagg cagcagtcca ccgctgtcag ggtacgatag
     6901 gtatcttccg agaagtcttc gtgccccggg gtgtctagca ggttaaccag gcaatcgtga
     6961 tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc
     7021 cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccagc ggtctgaatg
     7081 gcctgtccga acagcagcac cttctcggtg atggtagttt taccggcgtc cgggtgagaa
     7141 ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt
     7201 ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat
     7261 tttacccatc aacggggggg aggcaatctg tgtttacaca ggagctgctc cagttcgtgc
     7321 aacgatgaaa cggtccaggt gggcgcgatg ccttctggtt gctcgcgatg gtgcgcattc
     7381 agccagcaag tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggctgtgtcg
     7441 ccaaccatca gcacgcgtga acggtcagga ttgcccgcca gttccagcgc ataatcgaaa
     7501 attttcttat tcggtttggc aatgccaact tcttcggaaa tcaccagcaa atcaaagtaa
     7561 tcacgcaggc ccgtccgttc cagacgcact tgctgcaagg cgctaaaacc gttggtgatg
     7621 atgccgattt tagcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc
     7681 gtgcagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc ttcaactttc
     7741 agccgttcgg cccagctctc gaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg
     7801 ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg
     7861 gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta
     7921 aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt
     7981 acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt
     8041 aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag
     8101 cgtttgaagc acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga
     8161 tcagatgttt cagcagcacc cttcccaatc cctgacgctg atagtcagga tcgaccacaa
     8221 tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc gccattttgc
     8281 cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca aaggtttttt
     8341 cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg
     8401 tttcgaggga agaaatcgtg ttcatatgtg caaatctgtt gccataacgc ggcgcgtgcc
     8461 gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat
     8521 agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg
     8581 attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca
     8641 tcgctcacta aaggatcggt cagggcggga agatcgtttg ccaccatcac cagacggacg
     8701 tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac
     8761 tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc
     8821 tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat
     8881 cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct
     8941 tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg
     9001 atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccacc
     9061 actggcaggt attaagccgc cagatggggg ataacgcccg tttcagtctg gtcgccacgg
     9121 tggatgacgt cgcagattgc gatacgctga tttactactg gccgaagaac aaaccggaag
     9181 cccagttcca gttgatgaat ttactttctc tgctgccagt ggggacagat atttttgtcg
     9241 ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt
     9301 tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc
     9361 cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg acggtcaaaa
     9421 cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga
     9481 cgttaactcc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt
     9541 cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc
     9601 cagcggtaga agccagccgc gcaacacttg cggccaactg tgttgaaggt gaagtctttg
     9661 ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt
     9721 tccacgatgg gatgcaaacc agcctgaatg cggcgcaaac gctgattcgc ggcgcggtgc
     9781 gtcatcttaa tagtggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttatccgg
     9841 acgtgctgga tgagacattt ggcttccatg aagtgattgc gcaaaccggg cgtttcaagg
     9901 tatatcgcgc cattatgacc cgccaggcga aaaaaggtta agtatcccgc cgcaagcctt
     9961 gccggatgcg gcgtaaacgc cttatccgac ctacaatacc tgcacgttag cccttcgtag
    10021 gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg
    10081 cacgctagtt tcctgatgga catttttcca gcaattacac ctccggcgat aattaactat
    10141 tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt
    10201 gatggtatgc gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgtcctta
    10261 cggacgtggg ggttcaagtc ccccccctcg caccaaaaac cacgttgata ttgctcgcac
    10321 tgggcgaagg tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt tcttacggac
    10381 gtgggggttc aagtcccccc cctcgcacca aacgaggcga tatcaaaaaa agtaagatga
    10441 ttgtgcgaag gtggcggaat tggtagacgc gctagcttca ggtgttagtg tccttacgga
    10501 cgtgggggtt caagtccccc ccctcgcacc aattatcttt acttcctttc ttgtttcttc
    10561 cttgattttt catccctgat tcaaattcat tatcatcagc atgatggcag caataagcgc
    10621 caggcacgat ggtagcaaaa cgtaatgccg taactgtttg tgatacagca tcaccggcgt
    10681 caatactaat cccatcagaa tgacaatccg ccactgatcc ggcgataaat ccgcatacat
    10741 cagccctgca atcagcaatc ccggcagcaa cactccccag ccactgccca gcgtacgttg
    10801 caacatggtt tcatctcttt cattgataat gataaccaat atcatatgat aatttttatc
    10861 atttgcaagc cagagaaatc ccttgctatc gggtaaacct atcgctagga ttagcaatca
    10921 ttatcattta gattactatc ccgattatgg cctatcgttc cgcaccgctc tatgaagata
    10981 tcatctggcg aacgcatctc cagccgcagg atgctgggct tgcacaagcc gtacgtgcga
    11041 cgatcgccaa acatcgtgaa catttgctcg agtttatccg cctggatgaa cccgctccgc
    11101 ttaacgccat gacgctggcg caatggtcat caccgaatgc gctaagttct ctactcgcgg
    11161 tctattccga tcatatctat cgcaaccaac caacgatgat ccgcgagaac aaaccgctga
    11221 tctcactatg ggcacaatgg tatatcggcc tgatggtgcc accattaatg ctggcgctac
    11281 tgacgcagga aaaggcatta gatgtgtcgc cggaacattt ccatgctgag tttcacgaaa
    11341 ccggacgcgt cgcctgtttc tgggtcgatg tgtgtgaaga taaaaacgca acaccacatt
    11401 cgccgcagca gcgaatggaa acgttaatca gccaggcgct ggttccggtt gtgcaggcac
    11461 tagaagcgac cggagagatc aacggcaaac ttatctggag taataccggt tatttgatca
    11521 actggtatct cactgagatg aagcaactgc tcggcgaggc gacggttgaa tcgctgcgcc
    11581 atgccctctt ttttgagaaa acgctcacga acggtgaaga taatccactc tggcgtaccg
    11641 tcgtgctgcg cgatggcctg ctggtgcgcc gaacctgctg ccagcgttat cgcttaccgg
    11701 atgtgcagca atgtggcgat tgtacgctga aatgaaagca ctgccgggga agtaaacccg
    11761 gcatcatgcg gattaatagg gatgcaacac attacttgct tcgcaatgcc gcatacataa
    11821 cagaatatct gcggcctcca acaaccctgc atcagaactg acgcccagtt tcgacatcac
    11881 attaaattta tgtgcacgga tcgttttgat attgcgttta agctgctcgg caatttgtgt
    11941 cattgagtag ccacgcgaca taaagcgcaa tatttctctc tccgtcgggc ttagcgtccg
    12001 gctttggtta atgtaccact gattgttcag tcggtcggtc gcctgacgta caccatttaa
    12061 tgacaaaaag agttcctgat gaaaaatctc cagcgttgac gctttactta atacaccgtc
    12121 cagcggtgat ggcgacaatg aaccaatcag ccgagcttca atatcatcat ccgcaataac
    12181 taaacgccgg gtacgcggaa acttaatcgc cagttcagtc aggcaagata atccctctct
    12241 gcgctcactt ctcatggcag aaaaagaaaa atgaccgccg caaatgagat ccgcgacatg
    12301 gcatcctgaa atgactcctg actggtatag gtatggagct tataagggtt acccgcaaac
    12361 tttctgaata aactctcaat accgatactg ctcatgacgc atttttctat gagtgcgaca
    12421 tttctcttct taattcggct gtgttccatt ctgcggcatc tccataaaca gaaggcaagt
    12481 taagttctct tgcaccctgc gcgctattta tccatgcata catttcagcg ttgctgtgaa
    12541 ttgccagtcg acgcatcgca ctgttttttt gggcgctgat agttttatta cttttcttaa
    12601 gcaacgaaac tatctggttg atgccccatc ctttacctaa tagccgcagt accttgcgct
    12661 ctgaaagagt gagaatgacg gagcgatagc taaaatcatg agtttcattc attgccggag
    12721 aggtcagcat ggcgctgata cgctctgcgt gcgtattgcc ggaacgtacg gtcttgacca
    12781 gattctcaat gggttcaaca tcagacaata acgtcgccgt agggcacatg agcaattcta
    12841 ctgcctggga ataccaggag cgtgacacca tgaaaaccca gtgaatttcc cgatattgtg
    12901 agattaaaga ataataatgt tcacaaacag aacgtggatc ttcactttca ccggctaaat
    12961 cagcaatgac taatccggaa cgacgtaatt gtaataaggt aaggtcctcc gcagaggcgc
    13021 tggatattat ttcatattca gggaagtgag tcctcatgac ctcttttaac cctgcttgca
    13081 taacaggtat tttactgata acaattccat ttttgcagca tcctggcaac atatttcctc
    13141 cacatccact gtctaaaaat catgatggtt tattcaggat attcattcct taaatgtttc
    13201 cagcaaatta atagattaag cgaataaatt atccttagca ttcactttca tatcagcata
    13261 ctcctgctgc acaacaactc cactgcatgt gtcagaaggt tagcatcaaa tgataacctg
    13321 gtgaaatagc ctggagacat ttaaaatatc tacattaagt tgttaggaat acttaatgaa
    13381 aaacagaaat cacattcatt taatttataa ttaaatgata tcccttcccg cagaatgaaa
    13441 aatcacacaa ccaatattta ttcaatgaaa tctgatggtt ttaacttgtt aacattaaaa
    13501 aacgtgcccg gttatctatt atcctgattc gtaaaggcca aacaataaat tcattatagt
    13561 gagaatattc atgtgatagt ttgtttgcgg cgagagataa ttcgcttttt atcaccgagc
    13621 ttgtcccgat aaccaggatt aatcagcgca attttgatct taacctaccg caaaacaacc
    13681 gtgcttcatg atacaaatgg attctcatta tctgaacaat acgcagcacg tctatgacaa
    13741 agggcgagtt atgcaaactg agcaacagcg agccgtaaca cggctttgta tccagtgtgg
    13801 attatttctt ttgcaacatg gtgcggaaag cgcgttggtt gatgagcttt cctcacgact
    13861 gggtcgggcg ttggggatgg acagcgtcga aagttctatc tcttcgaacg ccatagtgct
    13921 gacaaccatt aaagatgggc aatgcctgac atcgacacgt aaaaatcacg atcgcggcat
    13981 taatatgcat gtggtgactg aagtccagca cattgtgatt ctggcggagc accatctgct
    14041 ggattacaaa ggcgtagaga aacgatttag ccaaattcag ccattacgtt acccaagatg
    14101 gctggtagcc ttaatggttg gcctttcttg cgcctgtttc tgcaaactca ataaaggtgg
    14161 ctgggatggt gccgtcatca ccttctttgc cagtacggcc gcgatgtata tccgccagct
    14221 gctggcacaa cgtcatcttc atccgcagat caacttttgc ctcaccgctt tcgccgccac
    14281 caccatttcc ggattgcttt tgcaacttcc cactttcagc aatacaccca ctatcgcgat
    14341 ggccgccagc gttctgctgc tggtgccggg atttccgttg attaatgccg tcgccgatat
    14401 gtttaaaggc cacatcaata ccggactggc acgctgggcg atcgccagtc tgctgacact
    14461 ggctacctgc gtcggcgtgg tgatggcact gacgatttgg gggctacgcg gatgggtgtg
    14521 atcgagtttc tgtttgcatt ggcgcaggat atgatcctcg ccgccattcc agcggtcggc
    14581 tttgcgatgg tgttcaacgt tcccgttcgg gcattacgct ggtgtgcgct gcttggcgcg
    14641 attggtcatg gttcacgaat gatcttaatg accagcgggc tgaatattga gtggtcaacc
    14701 tttatggcct ccatgctggt cggcaccatt ggtattcaat ggtcgcgctg gtatctggcg
    14761 catccgaaag tgtttaccgt ggcggctgtt atccctatgt tcccgggcat atcggcttat
    14821 accgcaatga tttcggcggt aaaaatcagc cagttaggtt acagcgaacc gttgatgatt
    14881 accctgttaa ccaactttct tacagcttca tcgattgttg gtgcgttatc catcggtctt
    14941 tccattcctg gattatggtt gtaccgcaag cgccctcgcg tataaaattg cctcgcttaa
    15001 agcacacgga tgagagacag cctcctctcc tccgtgtgtt actataaaag ttatctccct
    15061 tctcgttcat cgttccatat ttgagaaaca gtatgtcttc cagagttttg accccggacg
    15121 tcgttggtat tgacgccctg gtacacgatc accaaaccgt tctggcaaaa gctgaaggcg
    15181 gtgtggttgc cgtatttgct aacaatgccc cggcgtttta tgccgtcacg cctgcacgcc
    15241 tggctgaact gctggcgctg gaagaaaagc tggcgcgtcc gggaagcgat gtcgctctgg
    15301 acgatcaact ctatcaggaa ccgcaagccg ctcccgttgc tgtacccatg gggaaattcg
    15361 ccatgtatcc ggactggcaa cccgatgccg attttatccg cctggcggcg ctatggggtg
    15421 tggcactaag agagccggtg accaccgaag aactggcctc attcattgcc tactggcagg
    15481 cggaaggtaa agtctttcac catgtgcagt ggcaacaaaa actggcgcgc agcctgcaaa
    15541 tcggtcgtgc cagcaacggc ggactgccga aacgagatgt gaatacggtc agcgaacctg
    15601 acagtcaaat tccaccagga ttcagagggt aacgatgaaa aacgttggcg acctgatgca
    15661 acgcctgcaa aaaatgatgc ctgcccatat caaacctgca ttcaaaacgg gtgaagaact
    15721 actggcctgg cagaaagaac aaggagcgat ccgctccgcc gctctcgaac gtgaaaatcg
    15781 ggcgatgaaa atgcagcgca cctttaaccg ctccggtatt cggccactgc atcagaactg
    15841 ctcctttgag aactatcgcg ttgagtgtga agggcagatg aatgcgttaa gcaaagcgcg
    15901 ccagtacgtc gaagagtttg acggcaacat cgccagcttc atcttttctg gtaagccagg
    15961 aaccggcaaa aaccatctgg cggcggcaat ctgcaacgag ctgctgctac gcggtaaatc
    16021 cgtattgatc atcaccgtgg ccgatattat gtcggcgatg aaagatacct tcaggaatag
    16081 cggtaccagt gaagaacaac tgcttaacga tctgagcaac gttgatctgc tggtgatcga
    16141 tgagatcggc gtgcagaccg aatcgaaata cgaaaaagtg atcatcaacc agatcgtcga
    16201 tcgccgctct tcttccaaac gcccaaccgg gatgctgacc aacagtaata tggaagagat
    16261 gaccaagttg ctgggcgaac gcgtgatgga ccgtatgcgc ctgggtaaca gtttgtgggt
    16321 gatcttcaac tgggatagct accgtagccg ggtaacaggt aaagagtatt aatccccgtt
    16381 tctggcggta tactgcgaca caattccacc ttgagtaatg atgatgaaaa ctgttaaaca
    16441 tcttctgtgc tgtgccattg ccgccagcac attaattccc accggggtgc atgctgcgtc
    16501 ctggaaagat gcgctctcca gcgccgccag cgaacttggc aaccaaaaca gcacgacaca
    16561 ggaaggcggt tggtcgctcg cgtcattaac taacttgctt agcagcggga accaggcctt
    16621 aagcgcagat aacatgaaca acgccgcagg cattctgcaa tactgcgcga agcaaaagct
    16681 ggcgtcggta accgatgccg aaaacatcaa gaaccaggtg ctggaaaagc tgggcctgaa
    16741 cagtgaagag caaaaagaag acatcaacta tctggatggc attcagggtt tgctgaaaac
    16801 caaagatggt cagcaactca atctggataa catcggaacg actccgctgg cagaaaaggt
    16861 gaaaaccaaa gcctgcgatc tggtgttaaa gcaggggctg aacttcattt cctgaaccgc
    16921 gaaacgccgc gttttagctg ttacccggct gacgcggctc tctcctgccc ttttccctgc
    16981 ttctggacgg ttttccccct gttacacggg aagcgaccgc gatcaaaaaa gtctgtttat
    17041 aaactaattc ctaacaacat cgcatttttt tgacactaga attgcagcaa tatggcgacg
    17101 ccattacatt gttcttctca aaaagcttaa acgttggcta aacggcccgg tttagtgagg
    17161 atcgcccgtt gtcagaacta ctctctttcg ccctttttct cgcctctgtg ctgatttacg
    17221 catggaaagc gggacgtaac acctggtggt ttgcagccac attaacggtg ctggggctat
    17281 ttgtcgtttt aaatatcacc ctgtttgcca gcgactattt tactggcgat ggtattaacg
    17341 acgcggttct ctatacctta accaacagcc tgaccggtgc tggcgtcagc aaatacatcc
    17401 tgccgggtat cggcattgtg ctggggctga cagcggtgtt cggtgcgctg ggctggatcc
    17461 tgcgccatcg tcgccatcat ccgcaccatt ttggttacag cctgctggcg ctcttactgg
    17521 cgctgggttc ggtggacgcc agcccggcat ttcgtcagat aacggaactg gtgaaatccc
    17581 agtcacgcga tggcgacccg gactttgcgg cttattataa agagccgtcg aaaactatcc
    17641 ctgacccgaa actcaacctg gtttatatct acggcgaaag tctcgagcgg acctattttg
    17701 ataacgaagc tttcccggat ctcacgcctg aactgggcgc gttgaaaaat gaaggcctgg
    17761 atttcagcca cacgcagcag ctaccaggaa cggattacac gattgcgggc atggtggctt
    17821 ctcagtgcgg cataccgctg tttgccccct ttgaaggcaa cgcctccgcc tctgtctcca
    17881 gcttcttccc gcagaacatc tgtctgggcg atatcctgaa aaactcgggt tatcagaact
    17941 atttcgtgca gggcgcgaat ctgcgttttg ccggtaaaga tgtgttcctg aagtcgcatg
    18001 gcttcgacca cttatacggc tcagaagagc tgaaaagcgt ggtggccgac ccgcactatc
    18061 gcaacgactg gggattctac gacgataccg ttctcgatga agcgtggaaa aagtttgaag
    18121 agctttcccg ctcaggtcag cgattctcac tgtttaccct gacagtcgat acccatcacc
    18181 cggatggttt tatctcgcgt acctgtaacc gcaaaaaata tgattttgac ggtaagccga
    18241 accagtcatt cagcgcggta agttgcagcc aggagaatat cgcggcgttt atcaacaaaa
    18301 tcaaagcgtc accgtggttt aaagataccg tcatcgtcgt ctcttctgac catttagcga
    18361 tgaacaacac ggcgtggaaa tacctcaata agcaggatcg caataacctg ttttttgtca
    18421 ttcgtggcga caagccgcag caagagacgc tggcagtgaa gcgtaacacg atggataacg
    18481 gcgcgacggt gctggacatt ctcggtggcg ataactatct cggacttggt cgtagcagtt
    18541 tatccgggca gtcgatgtcg gaaatcttcc tcaatatcaa agagaaaaca ttggcgtgga
    18601 agccggatat catccgcctg tggaaattcc ctaaagagat gaaagagttc accatcgacc
    18661 agcagaaaaa catgattgcc ttctcgggta gccatttccg tttgccgctg ctgttgcggg
    18721 tttcagacaa acgcgtggaa ccgctgccgg aaagcgaata ctcagcaccg ctgcgtttcc
    18781 agctggccga tttcgctcca cgcgacaatt tcgtctgggt tgaccgttgc tataagatgg
    18841 cacaactctg ggctccggaa ctggcactct ccaccgactg gtgtgtctcg caagggcagc
    18901 ttggcggtca gcaaattgtt cagcatgttg acaaaacaac atggcagggc aaaacggcat
    18961 ttaaagatac ggtcatcgac atggcgcgtt ataaaggcaa tgtcgatacg ctgaaaattg
    19021 ttgataacga tattcgctac aaagccgaca gtttcatctt caacgtcgcc ggtgcgccag
    19081 aagaggtgaa acagtttagt gggatttccc gtccagagtc gtggggccgc tggtccaacg
    19141 cgcagctggg cgatgaagta aaaatcgagt acaagcatcc gctgccaaag aaatttgacc
    19201 tggtgattac cgccaaagca tacggcaata acgccagccg tcctattccg gtacgcgtag
    19261 gtaatgaaga acaaaccctt gtgctgggca atgaagtgac caccaccaca ctgcatttcg
    19321 ataacccaac cgatgccgac acactggtaa ttgtgccgcc ggaacctgtc tcaaccaacg
    19381 aagggaatat cctcggacac tcgccgcgta agctcgggat cggcatggtg gaaattaaag
    19441 tggtggaacg tgaaggataa ttcctgactg atgtgtaggt cgcataaggt gcgacctaca
    19501 caccttctgc ggcctacaaa acatcattga gaaacagaaa aaaacagcgc ccaacacttc
    19561 cccggacgct gcatggcaac tttaataatc tcgccttatc agaaagtaat tacaccctta
    19621 atcaactcac gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt ggcgaacgga
    19681 tagcgatggg ttaacatcat gtcagcagtg atttttcctt ccgccatcag tcgaccgact
    19741 ttagcaaaat cttccggcgt ggcgttgcgg ctacccatca tcgtcgtttc ttttttatgg
    19801 aattccggat cggagaactg caactcacct ttaaacaggc cgacaaatac caccgtgccg
    19861 ccgtgacgaa tcaaattcac ggtgttattc atcgcatgtt gattacctgt cgcgtcgatc
    19921 actttctgcg ccagcgaacc accaaactgc gcccgtagct gcgcgtcaaa atcttcggct
    19981 gacgggtcca gtaaaggtaa ttccagacgc gttgccacat gttcacggcg cgccggactg
    20041 gtatccgcca ccaccacctg tgcgccatcg gctttagcga ttgccgccgc gcccagaccg
    20101 attggccccg ccccgaccac cagcacctgc tcgccgggag caatggctgc gcgacgcacc
    20161 gcatgagcgc taatagcgaa aggttcaatc aatgctgccg cctgcgggtc aataccgtct
    20221 gcgggcaaaa tgttcgccac cggcacgctc aaatactcac taaaaccgcc atcctgatgc
    20281 acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc cgcttttaca cgccgggcat
    20341 tgctgacagg caacataagg gatcacagca acttgctgac catttttaag atcagcaata
    20401 tttttaccca gcctaacaat ctccccacat atttcatggc ctaaaacacg tggataacta
    20461 aaaaatggtt gatttccacc ccaggcatga atatctgtac cgcaaatccc gacagactta
    20521 atttttatta atgcttcatt gtcacccgga ataggtatct cgcgttgttt ccagactaat
    20581 tctttcggct gctggcaaat taaaacattc atcgtagaca tgattcggct ctccattttt
    20641 tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat ctgttttgtg
    20701 aaaacacacg cataaaaatg ttttaaatcg ggttttaatg gactcacaag gaggaaaata
    20761 tgagtcgttc acaaaattta cgccacaatg tgattaacca ggtcattgat gatatggcgc
    20821 ggggtcatat cccttcccca cttccatcgc aaagtgcgct ggcggaaatg tacaacatca
    20881 gccgcaccac agtgcgtcat attctcagcc atttacgcga atgcggcgtc ttgacgcagg
    20941 ttggaaacga ctatgttatt gcgcgtaaac ctgaccacga tgacggtttc gcctgtacta
    21001 ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg ctgataaacc
    21061 agcgccagtt acgcccaggg gaaacttttt ctgaactgca actggctcgg gccgcgggtg
    21121 tcagcccggt ggttgtaaga gaatatcttt taaaattcgg ccgttataat cttattcaaa
    21181 gcgaaaagcg cggccagtgg agcatgaagc aattcgatca atcctacgcc gagcagctgt
    21241 ttgagctacg cgaaatgctc gaaacacatt cacttcagca ctttctcaac ctgcctgatc
    21301 atgatccccg ctggctgcag gcgaaaacca tgctcgaacg tcatcggcta ctgcgtgaca
    21361 acatcggcaa tagctttcgc atgttctcgc aacttgacag agattttcac tccttactgc
    21421 tttcagctgc cgacaatatc ttttttgatc aatcgcttga gagcatctca gtcatctttc
    21481 attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt gccgtcgatg
    21541 agcatatgac cattctcagc gctctgattt gccgcagtga cctcgatgcc acccttgcgc
    21601 tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat gagaacacgc
    21661 gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgagtgca cggttttatc
    21721 aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg actaaaaatg
    21781 tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc cgttaatgtc aaccttgcgt
    21841 aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt ggaaaaagaa
    21901 aatatcacca tcgatccgcg ttcttcattt actccatctt cgtcggcaga tattcccgtg
    21961 ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac caccgccatg
    22021 ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct gtcggtagca
    22081 aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc tttgctctcc
    22141 gtgttttcac tcgcttacgg gattgcgcaa cttccttgcg gcccactatt ggatcgtaaa
    22201 ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca ggcaatgtct
    22261 ggcatggtgc acaactttac gcagttcgtg ttggtgcgta tcggtatggg gattggtgaa
    22321 gcgccgatga acccatgcgg tgtaaaagtc attaacgac
//
LOCUS       DAJTNA010000058        21309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTNA010000058 DAJTNA010000000
VERSION     DAJTNA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21309
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.059"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..777
                     /gene="umuC"
                     /locus_tag="OO161_001833"
     CDS             <1..777
                     /gene="umuC"
                     /locus_tag="OO161_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5462926.1"
                     /translation="DLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLAD
                     TDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQA
                     ICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIIN
                     AATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNA
                     KEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(823..1353)
                     /gene="dsbB"
                     /locus_tag="OO161_001834"
     CDS             complement(823..1353)
                     /gene="dsbB"
                     /locus_tag="OO161_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5462927.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1499..3040)
                     /gene="nhaB"
                     /locus_tag="OO161_001835"
     CDS             complement(1499..3040)
                     /gene="nhaB"
                     /locus_tag="OO161_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5462928.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLIEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMR
                     WIATL"
     gene            3262..3981
                     /gene="fadR"
                     /locus_tag="OO161_001836"
     CDS             3262..3981
                     /gene="fadR"
                     /locus_tag="OO161_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5462929.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4033..5565)
                     /locus_tag="OO161_001837"
     CDS             complement(4033..5565)
                     /locus_tag="OO161_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5462930.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            5895..7193
                     /gene="dadA"
                     /locus_tag="OO161_001838"
     CDS             5895..7193
                     /gene="dadA"
                     /locus_tag="OO161_001838"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5462931.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7203..8261
                     /gene="dadX"
                     /locus_tag="OO161_001839"
                     /pseudo
     CDS             7203..8261
                     /gene="dadX"
                     /locus_tag="OO161_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(8648..10384)
                     /locus_tag="OO161_001840"
     CDS             complement(8648..10384)
                     /locus_tag="OO161_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5462932.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10479..11393)
                     /gene="ldcA"
                     /locus_tag="OO161_001841"
     CDS             complement(10479..11393)
                     /gene="ldcA"
                     /locus_tag="OO161_001841"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5462933.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            11493..12104
                     /gene="emtA"
                     /locus_tag="OO161_001842"
     CDS             11493..12104
                     /gene="emtA"
                     /locus_tag="OO161_001842"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5462934.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12106..12840)
                     /locus_tag="OO161_001843"
     CDS             complement(12106..12840)
                     /locus_tag="OO161_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5462935.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQIKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13041..13295
                     /locus_tag="OO161_001844"
     CDS             13041..13295
                     /locus_tag="OO161_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5462936.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            13474..13914
                     /locus_tag="OO161_001845"
     CDS             13474..13914
                     /locus_tag="OO161_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462937.1"
                     /translation="MIQKINILDSSQSGVKNGAYSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(13993..15565)
                     /locus_tag="OO161_001846"
                     /pseudo
     CDS             complement(13993..15565)
                     /locus_tag="OO161_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
     gene            complement(15885..17303)
                     /gene="dhaM"
                     /locus_tag="OO161_001847"
     CDS             complement(15885..17303)
                     /gene="dhaM"
                     /locus_tag="OO161_001847"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5462938.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLYTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(17314..17946)
                     /gene="dhaL"
                     /locus_tag="OO161_001848"
     CDS             complement(17314..17946)
                     /gene="dhaL"
                     /locus_tag="OO161_001848"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5462939.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(17918..19027)
                     /gene="dhaK"
                     /locus_tag="OO161_001849"
     CDS             complement(17918..19027)
                     /gene="dhaK"
                     /locus_tag="OO161_001849"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5462940.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLGNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGKLGESNVTEQNSNC"
     gene            19255..21174
                     /gene="dhaR"
                     /locus_tag="OO161_001850"
     CDS             19255..21174
                     /gene="dhaR"
                     /locus_tag="OO161_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5462941.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSAFLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
BASE COUNT         5328 a         5323 c         5527 g         5131 t
ORIGIN      
        1 gatttatcaa atctggaacg ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc
       61 tgggggattg gacggcggat cagcaaaaaa ctggacgcga tggggatcaa aaccgttctt
      121 gatttggcgg atacagatat ccggtttatc cgtaaacatt ttaatgtcgt gctcgaaaga
      181 acggtgcgtg aactgcgcgg tgaaccctgt ttgcaactgg aagaatttgc accgacaaag
      241 caggaaatta tctgttcccg ctcgtttggt gaacgtatca cggattatac gtcgatgcgg
      301 caggccattt gtagttacgc tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat
      361 tgtcggttta tctccacatt tattaagacg tcaccatttg cgctcaatga accttattac
      421 ggcaatagcg cgtcggtaaa actgctgacg cccactcagg acagcaggga tatcattaac
      481 gccgctacgc gatctctgga tgccatctgg caagcgggcc atcgctacca aaaagcgggc
      541 gtgatgctgg gggatttctt cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac
      601 gcaccgcgcc ccgggagtga gcaattgatg acggtaatgg atacactgaa tgctaaagag
      661 ggcagaggaa cactctattt tgccgggcag gggatccaac aacaatggca gatgaagcga
      721 gccatgcttt caccacgtta tacaacgcga agttctgatt tactgcgggt caaataaata
      781 tagcggcagg aaaaaagcgc tcccgcagga gcgccgaatg gattagcgac cgaacagatc
      841 acgttttttc gctttaaacg gctgggaaat catcaccagc accgcgacaa tcaggtaagc
      901 gataaaaata ccgagcagcc actgcggcat ttccagacct aaaaaatccc actgacgctc
      961 ggcgcaatcg ccagaggcga caaacacttg cggcacccat ttatccagcg gcagccattc
     1021 cgggaaacga accataaaat cacaggtggc aaacggcgaa ggatagagct gaagcatggt
     1081 gtgctcgtaa gttaactgca caccgcggaa cgcactatac aaccagataa ccatcgctac
     1141 ataacgcagc ggagttttcg gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa
     1201 taacgcgcag cgttcataaa tacagagcac gcaaggtttc agtaacatca catgctggaa
     1261 ccacagcgcc gtcagttcca gtgccagagc agtaaacgcc atcaacagcc acgcgccccg
     1321 gccttgtgaa cattggttca aaaatcgcaa cataatcatt tccctgcaat atgcatagag
     1381 tgcgcagttt aaaccaattc attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa
     1441 ttggacaatt atcgggcgaa aaggcaaacc gggcatttat atgcccggta agttgttatc
     1501 aaagcgttgc tatccagcgc atttgcataa accattcggt tacaggggca agcgtaaact
     1561 cgacgcagag cagcccgacg agtgtcagga cgagggtgta aggcagggcc atccacacca
     1621 tgcggccata agagaggcga atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg
     1681 cagcctgacc gttcggcgta gcgacagagg gcagattggt accggtatta atggcgaccg
     1741 ccagcagctc gtattgcttc aacgtgatag caccactttc cattgccgct ttcgcttcgt
     1801 tgatataaat cgtccccacg aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa
     1861 tatagaacaa cgacagctga gcatgttccg atgcctgcaa cacaaactga ataatcggcg
     1921 aaaacagttg ttggtcgata atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg
     1981 tgaatggcag agattcggtg aaggctttac cgatagcatg ttcatcggtg acaccggtca
     2041 atgatgtcgc cagaataatg accgacaaac caatcaagcc aacctccgcc agatgcaacg
     2101 ccagcgcagt caccagccag acgccgataa tcgcctggac aatcagacgg attttatcct
     2161 gacgggtacg ctggtggcgg ctttgatcgt caaactgttg caacacttcg cggactttct
     2221 ccggcagcgt ttcaccgtag ccaaaccaac gcagcttctc tatcagcagg caggttaaca
     2281 ggccacaaat cagaaccgga acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat
     2341 gccagccagc cgctttagcg atgatcaggt tctgtggttc gcccaccatg gtcattacgc
     2401 cgcctaatgc ggtgccgaca ccggcatgca tcatcaggct acgcagaaag ccacggaact
     2461 gttccagaac cactttgtaa tgcttgtcga tatgactatc gtcttgcagg tcggtatctt
     2521 cagtacggga agaggctacg cggtgataaa taccataaaa accgactgca acgctgatca
     2581 ccaccgccac gacggttaag gcatcgagga acgcggagag gaacgccgcc gccacgcaaa
     2641 aagagagcga cagcagcatt ttggaacgaa tgcttaacag caaacgggta aatatgaaca
     2701 gcaatagctg tttcataaaa tagatacccg ccaccataaa catcagtaac agcaagactt
     2761 caagatttgc cgccacctct tcacggacgt gttccgcgct ggtcatgccg atgaataccg
     2821 cttcaatagc caacagacca ccggggagta gcgggtagca tttcagggcc atcgccagag
     2881 tgaaaataaa ttccgctacc agcaaccagc cagcgacgaa agggctgatg aggaaaatta
     2941 acggatttac gattaagaaa atgatgaggg cgagtttgta ccagtcgggg gactggccca
     3001 aaaaattgcg ccatagcgcg cggccccagg agatctccat gatggtttcc cttaccttac
     3061 aaataatcaa tgatgttttt atgtttaaac gcaaagctta acggtcaggc aggagtgagg
     3121 caagtcttga tagtcaaggg gaaagagatg cggaaaatga agccttgatc ccttttttct
     3181 tctttttgtc tgctatcagc gtagttagcc ctctggtatg atgagtccaa ctttgttttg
     3241 ctgtgttatg gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg tttcgcggaa
     3301 gagtacatta ttgaaagtat ctggaataac cgcttccctc ccgggactat tttgcccgca
     3361 gaacgtgaac tttcagaatt aattggcgta acgcgtacta cgttacgtga agtgttacag
     3421 cgtctggcac gagatggctg gttgaccatt caacatggca agccgacgaa ggtgaataat
     3481 ttctgggaaa cctctggttt aaatatcctt gaaacactgg cgcgactgga tcacgaaagt
     3541 gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca atatttccac tatttttatt
     3601 cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag tgctggctac cgctaatgaa
     3661 gtggccgatc acgccgatgc ctttgccgag ctggattaca acatattccg cggcctggcg
     3721 tttgcttccg gcaacccgat ttacggtctg atccttaacg ggatgaaagg gctgtatacg
     3781 cgcattggtc gtcactattt cgccaatccg gaagcgcgca gtctggcgct gggcttctac
     3841 cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga aacagtgcgt
     3901 cgctatgggc atgagagtgg cgagatttgg caccggatgc agaaaaatct gccgggtgat
     3961 ttagccattc aggggcgata atcccttccg tttaaaaagc aaacccctca aacgaggggt
     4021 tttttgttgt ttttacagat ttcccattct tggcgggcaa cgttccagca actcgacgct
     4081 gccatcttcg ttttgctgtt ccagcatcac atcaaatccc cacaggcgat gcacatgctt
     4141 cagcacttct ttgcgccccc gatccagcgg tgcgcgatta tgtggaatgt aacgcagcgt
     4201 cagcgaacgg tcgccgcgca aatccacgtt ccagatctga atattcggct ccagattact
     4261 taagttatat tgcgacgata accggttacg gatctcccga taaccttctt cattatgaat
     4321 agcggaaatc tccagataat tatgccgatc gtcatccagc acggtgaaga agcggaaatc
     4381 acgcatcact ttcggtgaca gaaactggct gataaaactc tcatctttga aatcacgcat
     4441 cgcgaaatgc agcgtttcca gccagtcgga accggcgata tccgggaacc agtatttgtc
     4501 ttcttccgtt ggcgactgac aaatccgttt aatatcctgg aacatggcga acccgagggc
     4561 atacgggttg atgccgctgt accacgggct gttatagggg ggctggaaga ccacattggt
     4621 gtggctgtgc aaaaactcca gcataaaacg ttccgttact ttcccttcat catacagatg
     4681 gttaaggatg gtgtagtgcc agaaggtggc ccagccttcg ttcatcacct gagtctgttt
     4741 ttgcggataa aaatactggc tcaccttacg cacaatacgc aggatttcac gctgccatga
     4801 ttccagcagt ggcgcatttt tctccataaa atagagcagg ttttcttgtg gttcggacgg
     4861 atagcggcgc gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg tacgccagag
     4921 catattgacc tgactttgta gatactcttc gcgacttttc tgccgggctt tctcttcttg
     4981 cagcgagatt ttttgcgggc gtttgtaccg gtccacgccg tagttcatca gcgcgtggca
     5041 cgagtccaga agccgttcta cttcatccac gccataacgt tcttcgcact cggtaatata
     5101 tttacgggca aaaatcagat aatcgacaat cgaactggcg tcggtccaac tgcggaataa
     5161 gtaattgttt ttaaagaaag agttatgccc atagcaggcg tgtgccatca ccagcgcttg
     5221 catggtaatg gtgttctctt ccatcaggta agcgatacac gggttagagt taatgacgat
     5281 ttcataggcc agtccttgct gaccgtgctt atacagccgt tcagtctcga taaacttttt
     5341 accgaatgac cagtgcggat agttaattgg catgccgacg ctggagtagg catccatcat
     5401 ctgttctgag gttatcactt caatctggtg cgggtaggta tccagccggt agagtttcgc
     5461 cacccggtct atctctgcca gataaacatc cagcaggtcg aacgtccagt cgggtccatc
     5521 gctcaaccgt gtggtgtcct tattcataga atcgatagtc gccatacgcg cacctcattg
     5581 ttgtcggcgc tctctgtgtg gagcacctca tttcaagcat agaacacctg ttaaaaaccg
     5641 cgtcgccgga gaattttttt ctttgcgatt tcttattatc agagtgccac taatccgctt
     5701 ctgaacggaa ttttatgctg gataaaaagg gcgttcagca ggagatacta aagacgccat
     5761 attgccgcag agtcagggag atgtgagcca gctcaccata aaaaagctgt atgttgaata
     5821 atattttcaa ctgagttatc aagatgtaat tagattatta ttcttttact gtatctaccg
     5881 ttatcggagt ggctatgcga gttgtcatac tgggaagtgg tgtggtaggc gttgccagcg
     5941 cctggtactt aaatcaggca ggacatgagg tcaccgtcat tgatcgggag ccgggggccg
     6001 ccctggaaac cagcgccgcg aatgccgggc aaatctcccc cggatatgct gcaccgtggg
     6061 cggcaccagg tgtgccttta aaagcgatca aatggatgtt ccagcgccat gcgccgctgg
     6121 cggttcgtct cgacggtacg cagtttcagt tgaaatggat gtggcaaatg ttacgtaact
     6181 gcgacaccag ccactacatg gaaaacaaag ggcggatggt gcgtctggcg gaatacagcc
     6241 gtgattgcct gaaagcattg cgcgccgaaa ccaatattca gtatgaaggg cgtcagggtg
     6301 ggacgctgca actgttccgt accgaacaac agtatgaaaa tgcgacccgc gatatcgccg
     6361 tgctggaaga tgccggcgta ccgtatcagc tgctggaatc cagccgcctg gcggaagtgg
     6421 agccagcgct ggcagaagta gcgcacaaac tgacgggtgg tctgcaactg cccaatgatg
     6481 aaaccggaga ctgccagctg tttacccaga atctggcgcg gatggcagag caggcggggg
     6541 ttaaattccg ctttaatacg cccgttgacc aactgctttg cgacggcgag caaatctacg
     6601 gcgtgaagtg tggcgatgaa gtgattaagg ccgatgcgta tgtgatggcg tttggttctt
     6661 actcgacggc gatgctcaaa ggcattgttg atattccggt gtatccgctg aaaggctact
     6721 cgctgactat tccaattgcg caggaagatg gtgcgccagt atccaccatt cttgatgaaa
     6781 cctacaaaat cgccattacc cgtttcgata accgcattcg tgttggcgga atggcggaaa
     6841 ttgttggttt taacaccgag ctgttgcaac cgcgtcgtga aacgctggaa atggtggttc
     6901 gcgatctcta tccacgcggc ggtcatgtcg agcaggcgac tttctggacg ggtttgcgcc
     6961 cgatgacgcc agacggtacg ccggttgtcg ggcgtacacg ctttaaaaat ctgtggctga
     7021 ataccggtca cggcacgctc ggctggacga tggcttgcgg ttccggtcag ttgttaagcg
     7081 atctgctctc tggtcgcacg ccagcgatcc catatgagga tctaagcgta gcgcgctaca
     7141 gccgtggatt tacgccatca cgtccgggcc atttacatgg cgcacacagc taaggaaacg
     7201 agatgacccg tccgatacag gccagcctcg atctgcaggc attaaaacag aatctgtcca
     7261 ttgtccgcca ggccgcgccg cacgcgggtg gtaaaagcga acgcttacgg gcatggtatt
     7321 gagcgtatct ggagcgcgct cggggccacc gatggctttg cattacttaa cttggaagag
     7381 gcaataacgt tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc
     7441 catgctcagg atctggatat ttatgaccag caccgcctga ccacctgcgt acacagcaac
     7501 tggcagctca aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa
     7561 gtgaacagtg ggatgaatcg gttgggcttc cagcccgatc gcgtgcttac cgtctggcag
     7621 cagttgcggg caatggcgaa tgttggcgag atgaccctga tgtcgcattt tgccgaagcg
     7681 gaacatcctg atggaatttc cagcgcgatg gcgcgtattg agcaggcggg gaagggctgg
     7741 agtgtcggcg ttcgttgtcc aattcggcgg cgactctgtg gcacccggaa gcgcattttg
     7801 actgggttcg gcctggcatt attttgtatg gcgcttcgcc gtccggtcag tggcgtgata
     7861 tcgccaatac cggattacgt ccggtgatga cgctaagcag tgagattatt ggtgtccaga
     7921 cgctaaaagc gggtgagcgt gtgggctacg gcggtcgcta tactgcgcgc gatgaacagc
     7981 gaatcggcat tgtcgccgca gggtacgccg acggttatcc gcgccacgcg cctaccggta
     8041 cccctgtttt agtggacggc gtgcgcacca tgacggtggg gaccgtctcg atggatatgc
     8101 tggcggtcga tttaacgcct tgcccgcagg cgggtattgg tacgccggtt gagctgtggg
     8161 gcaaggagat caaaattgat gatgtcgccg ccgctgccgg aacggtgggc tatgagttga
     8221 tgtgcgcgct ggcgctacgc gtcccggttg tgacggtgta acttgttgta agccggatcg
     8281 gaggcaacgt cttctgggtg caaaaaaaat catccatccg gctggtcagc aactgtagtt
     8341 gttaatgtga cagagccatt gcccatgata gtgtctatta aaaggatgga cactatttcc
     8401 ccggaacctg aactcaccgc acaggcgttc tacataaaac gcttacgctt cattgttgac
     8461 tcggagtcga cttcagataa atcacgcttt acccttgatg gagcctgtac atagatttgt
     8521 gtaattgcct gattttgata tgttcaattc aacatcaaat gaaggttaaa ttatggacga
     8581 caaacaattg caggctcagg ctgcgttcag caaagcatcg caaccggcga tagatgcttc
     8641 attaaattta agattcagct tcctcttcag ccacccgtac gccaatcttc aacacttcat
     8701 tatcttcttt ctcggccacc gtccagatca tcccggcaaa ctctacctgg tcaccgacaa
     8761 ccggtgccgc gcctaacaac tgctggacaa tttcacccag cgtttgctgc ttatcacgat
     8821 attctcgccc gtcttccagg ccatatatca gcgcaacatc agcatatttg gcgctggctt
     8881 cgagaatgaa gtcaccaaag aagcgttgat ccagcgcgac cggcggcgac tggctgaaca
     8941 gtttaccgag cgccggaaga tcgcgttccc gaccaattac acacaacaca tcgccttcgc
     9001 gcagtcgggt gctgccggta ggatgaagca actggttatc acgaaacagt gccgcaatac
     9061 gcgtctcttt tggcatatgc aaatcgcgca gtgccgcgcc cacgcaccat ttatcggcac
     9121 tcaattgata aacaaactgc tcccacggat tttccggatg aatatccagg ccaacgcgtg
     9181 acaccggacg tcccactggc ggaaccacca ctttggcttt tttagccgcc cacgagagtg
     9241 atgttccctg caagagcagt gaaaccagaa ccacaaagaa ggcgacatta aagaacagac
     9301 gtgcattctc cagccccgcc atcatcggga acacggccag gatgattggc accgcgccgc
     9361 gtaatcctac ccagctgata aacacgcgct cacgcagatt gaaaccgcgg aaggggagca
     9421 atccagcaaa taccgaaagg ggacgggcga agaatatcat ccatgccgac aaaatgagcg
     9481 ccggaatggc aataggcagc agatcgcttg ggttaaccaa tagccccagc accaggaaca
     9541 tggcgatttg cgccagccag gcgaggccgt cgaaattttg caggatgccg tagcggttgc
     9601 gaatcggccg attacccagc agaaaaccgc acagataaac agccagaata ccgctgcctt
     9661 ccagcgccgt ggttaaagca aaaattaaaa taccgccgct taatgccagc aatggatata
     9721 atccggcagg cagggcgatg cgattaatca tttgcagcag taaataacca ccgccaagtc
     9781 cgatgacaat cccgaggcca aattgctgca gaatatcgac cacgaacatc caactgacac
     9841 tgctttcatg ttgctggatc atcgcaatca gggtaatcgt cagaaagacc gccattggat
     9901 cattactgcc ggattcaatt tccagcgtcg agccaacacg ttcgttaagc cccttaccac
     9961 ccagcagaga aaagaccgct gcagcatcgg tagagccgac gatagcgccg attaataagc
    10021 cttcaatcaa atcaagatta aacagccacg ccgccatcat gccggttaaa ccagaggtga
    10081 taagcacgcc cagcgtcgcc agcgacagtg ccggtcctaa cgccacacga aaggaactgg
    10141 cctgagtgcg catcccgccg tcgagcaaaa taatcgccag tgccaggtta cttaccatgt
    10201 aggcgaaggg gtaattatca aacgggatac cgccgacgcc atcgactcct gccagcatgc
    10261 cgatcgccag aaaaataacc agaataggaa tgccaagacg ggaagaaaat gaactaagta
    10321 atatactgct ggtgacaagg atggatccta agatgaaaag gctaattatt gttgtggcat
    10381 ccaacggtcg gttactcctg attacgctgt ctcttatata aaccctacca tattagcggc
    10441 agagacagcg ttttacttag ccctgaagcg tgattttttt acattttaag aacaggatga
    10501 ccgctaatcg tcaactgagt gccttcctgg gtgttattca gaatggcatg tgcgcccaac
    10561 ggcagcgtaa ccgtgcgttg ttcatgacca aaatcgaggc cggtaatgag tggaatcgac
    10621 aggcgggaac gcaaaaacgc gtacactgac tccaggttgt aacctgcgtc ataatcattg
    10681 ggcgtgctgc cgctaaagct accgagaata atcgccttct gacgtggcaa aattcccgca
    10741 tgatagagct gcaacagcat acgttcgacg cggaaagggt gctcgttaat atcttccagt
    10801 accaggatac cgttctcaat ttttggcatc cacggtgtac caatcagtga aatcagcatc
    10861 gcaagattgc ctccccacaa cgtgccttcg gtctgacatg tcgggccttc cccttgccat
    10921 tcaatggtga aggttttatt gcgtaacgcc agccagaaat ggtgctcggt aaaggcgttc
    10981 agttcatccg caccaaagtt tgctaccagc atggggccgc taaaggtgat gacattgcct
    11041 tgcgccagaa gaccgcactg aatggcggta aaatcgctat gtccgcaaat gagtaacggg
    11101 tcatgttgtt ggcgagccac cagtgcctgc cagtcaatat ctgccagtaa acgactggca
    11161 ccgtaaccgc cgcgtacagc cagcacgatg gtgttgggtg tagtcagtct ggcaagggaa
    11221 ttgagatctt ccagacgctc cgtttccgta cccgcaaaac gctcacaacg acgggcaata
    11281 acctcgacgt tattgacttg atgccccgcg tcggtaaggc gctggatacc acgcagcgcg
    11341 gcgtgctgtt taatgctgta acccgatggg gcaattaagt gaaacagaga catggcaatt
    11401 ccttgctgac aacagaaacg aaatgtatat catgccgctt aggtgtgccg ttgtcacctc
    11461 aacggcgatt ccaggctata aggatagaag aagtgaaatt gagatggttt gcctttttga
    11521 ttgtgttatt agcgggttgt tcatcaaagc atgactatac gaacccgccg tggaacgcga
    11581 aagttccggt gcaacgtgcg atgcagtgga tgccaataag ccagaaagcc ggtgcagcct
    11641 ggggcgtcga tccacaattg atcacggcga ttatcgctat cgaatcgggt ggtaatccga
    11701 acgcggtgag taaatcgaat gccattggtt tgatgcagtt aaaagcttca acctccggac
    11761 gtgatgttta tcgccgtatg ggctggagtg gtgagccgac gaccagcgag ctgaaaaatc
    11821 cagagcgtaa tatttcaatg ggggcggctt acctgaatat tctggaaacc ggcccgctgg
    11881 caggcattga agatccgaag gtactgcaat atgcgctggt ggtgtcatac gctaacgggg
    11941 caggtgcgct gctacggact ttctcgtcag atcggaaaaa ggcgatcagc aaaatcaacg
    12001 atttggatgc tgacgagttc ctcgagcacg tagcgcgcaa tcatcctgcg ccgcaggctc
    12061 cgcgctatat ctacaaactt gagcaggcac tggacgcgat gtaaatcagt cgcgtacttt
    12121 gtccgctttc tcccgggctt cccgctcgag agagaaaata atccgttgta attgccgctc
    12181 caccgtcggg ctgacgttaa gaaaacggaa gctcagacgg ggagtggtga tggtttcatt
    12241 cttgccatca atcactttgc gctcgctgat ggagattaac tgggcgtcaa agtgaaaaac
    12301 accccattgc cccatgttga cttcaatctg agcgaagcgc atgccttctt gtaattcggc
    12361 aggctttgct gtttccagta atgcgcccat gccgcctaac gacaaatcat acaggcggaa
    12421 acgtaacgta ctgttatccg ccagtttgat ctggcaaaaa taaggcggat ggagtggggc
    12481 ggagatgcgg aaatatcggc gtcgttgtac aaaccataag gtgggaggcg gtacggtaat
    12541 aaatgccgga agctgcaagt attcactctg ctgtagttgt tcaacagtaa actcgacttt
    12601 cgcaccctga gtttcggcgg taatggtaat atgctgcgcc tttagcacgg cgatgttgtc
    12661 ttcggcttga ctgccgaaat ccagcaccag tttatccggg gttattgcca gtattttgct
    12721 gatcagctgc ccgccattcc aactgagacg caaaggaatt gcggctttgt gcaaatcgcg
    12781 taacacgccc aggacggcta acggattttg tttcaggaac tgctcatggt aatgactcac
    12841 gcggaaaaac tcctgactga caaactgtct ttggtttatc ggtcacagtt aacaaaactt
    12901 aatacaaatg cgtgaatatt ttttacatgt tgttcttaaa tcagccgaca tacgcccagc
    12961 atcgatcccg accttgccta tacttagagc gttgacgtaa gcatttcttg cgtccgattc
    13021 atcgaaacga gggcctgaac atgggaatta ttgcctggat tatttttggc ctgatagccg
    13081 gcattatcgc caagctaatc atgccggggc gtgatggtgg tggatttttc cagacctgta
    13141 ttctcgggat agtcggtgcg gtggtcggcg gctggctggc gaccatgttt ggcattggcg
    13201 gctccatcag cggttttaat ttgcacagct tcctggtggc ggtggtggga gctattctcg
    13261 ttctgggcgt attccgcctg ctgcgaagag aataagattt tcataaggcg gatagcgata
    13321 cagatgccgc tatccgcttt tcacatcaga acgtatattc gacacctgct ttacgggtga
    13381 aaaaaatcaa tatagcactt cgatatccga taaagtcggt tcccggtaac aacaacagaa
    13441 tcctttttta attattgttt cgttgttttt ggtgtgattc agaaaataaa tattttagac
    13501 agtagccagt caggcgttaa aaatggggcg tatagtgagg cggttttggc tgcgcttatt
    13561 gaacatttag aattgatcaa tccttctggc agattaagcc aaaatacccg ttcggcaatg
    13621 cttcagttac gcgaagagga atggtctgaa ttcttttttt ggttgctaaa ttctctggag
    13681 tgtctggatt atgtgattat taatttaact ccagaaagca aaaaaacgtt gatgagtgaa
    13741 caccgtaaca atattcaggt agcaatcgat gctttatata ggcaacgtcg gtgtaaatca
    13801 ccgggggatg atagtgaaac acttacccgt agaaatgacg ctatcttcgg caaccacgta
    13861 tggcaaacat ttgcgcaata cttcccgcca ggacttgaaa aaccgtctgt gtagaaaaca
    13921 ccatagctat ggtgttatct gccgacaacg ccatccggag aagccgaatg acgttgtcgg
    13981 agtaagcgct ggttaaggtg tgggttgtgc ctctttggtt gagggttgcg tcgttgctga
    14041 cttaacggtc ggacgcgtcg ccggaacatt gtcacacggt tgctctttcg ggcagatcaa
    14101 atccagcatt ttcagcgtca cgccattggt ccagccaaag ccatcctgta atggatattc
    14161 gccacctctt tttgcccgta gttttgtaat ccttctgtcg cgacccactg taacggtgcc
    14221 cagccatttg gcgcatccca ttgttgccca cttttcaccg acgtggtgtt caggccgccg
    14281 ggttgcagca gatgtgtttt cgtcgccgtc gccattttgt tggcgcgatc tttcgctgcc
    14341 gcattgacgt acagcgggaa cagggcggcc gcggttaact gattgcgcac tttatgactt
    14401 ttcaggtcgt aatcggcata ccagccttgt tgatcgttcc acaggtattt ttcgattcct
    14461 ttttgacggg cattcgccag cgtttcgtac tggtttgcca tcgcgttatc tcctgcagct
    14521 ttgctggcgc gggcgaggat tttttccatt ttaaacatca ggctgttcag atcgaccggt
    14581 acgatgctgg tggtgcgtaa ggtatttaac tgctgcgggt tatccatcca gcgcgagctg
    14641 aaatcccagc cagacgcagc ggcagagcgc aggtcgcggt aaatttcagt ggcaggtcga
    14701 ttcggattgc ttttggcggt ggcaatatct tccacccatg actctggtcg tggcgtatcg
    14761 cgatcgtccc agtagcggtt gagaagggta ccatcctgaa gtttgacaac gcgtttttcc
    14821 tgttgtccgg cttgcaggtt ttcaacaccg tccatccagt aagcatattc tttttgcatt
    14881 tgcggcaggt attgcttcaa cgcggcatcg ccttcatgct gcgccagtaa ctctaccatc
    14941 agggcaaaga agggcggttg cgagcggctt aaatagtaac tgcggttgcc gttgggaata
    15001 tgaccgtaag tgtctatttc atgagcaaaa ttggccacca tatccgcgac tttatcccag
    15061 tgaccgcttt cggcaagtcc taacatggtg aagtaactgt cccagtaata tacctcgcga
    15121 aaacgtccgc ccggcacgac ataaggttcc ggcagcggta acagagaatc ccatttttcg
    15181 gtgttttcgg tagaacgcgt taataccggc caaagtccgt caatatgttc gcgcagtgac
    15241 tgcccctctg gcggaacata tttctcgcct tctttcggca gggtgaaatt gacgttaacg
    15301 aaatggcgca gatcaaatcc gctctggttt tgctgcatcc gataatcagc ggatcgctgt
    15361 tcggcacggc atcggcaaag gttttttggt ccggaaaaag tttggcgttt tgcacatcat
    15421 taaacagcgg ccctaataaa atatcaggcg gctgtggtgt taccggtgtt tcttctgcct
    15481 gcaccgatag cgcagcgaaa cacaaaaaga tacaggctgg aattaacgcc attttttgcg
    15541 ggcgagaagg tgcgggggat ttcatcaatc attctccttt ggcgaaaccg aataaaacgg
    15601 tattcaagta tcagaaaacc ttagttcagg atcgcgctag ctgcatgatc gaaatctcat
    15661 tctgcgaact gtcaggcatt ttagaaaagg gaattaaagg gataaataac ggcgaaatag
    15721 agtgatcaaa taacgtctgt ttattagcca gccagcggcg ttgggttcgt gattagcgaa
    15781 gaaaaatcat cataaattgg tgaaaaatat aacaggtagt taaaaaccat tagtgctgag
    15841 taaattgccg gatgacatca gaacgatgcc atccgaacag tggcttaacc ctgacggttg
    15901 aaacgttgcg ttttaacgtc cagcgttagc gtttcttctg gttgtatcgc atacagtttc
    15961 tcaccctgct ggcaaatcca gccaatcccc agttcacggg cgattagggc gctgtgggat
    16021 accggacttc cggcgctaag gcagatacct tttacaaccg ccggatccag ttgcagtact
    16081 gtggaaggat aaatgttctc cgccagtaga atagttggcg agttaaactg cgggagttct
    16141 tctttcgttt gggtcaggtg gaccagggtg cgatgcagaa gatcgtccac atcaatatag
    16201 cgagcttgta gatattcatc atccagttgc tggtattgct ggctaagttc tttaagaact
    16261 tgctgccagg catattctgc cgtgcaatgt tcatgctgaa ggagttcgct tgccgccgcc
    16321 agcagttccg gatcatctaa cagtgtatgg tgaccagaaa agattgcggc aatatcgtca
    16381 agcccgctgg cttctgcttt cgctgttaac gtcatcagat ctaataacgt gaagtcaata
    16441 gcctggcgta atcgatcttg ttcttcttcc acggtcaggg ttgattttgc ctgtaccgta
    16501 tataaaactg gttgataata aaaggcttta cccgaaacag gcggaacggg acgcagagta
    16561 ggtggagcga cttcctccgt ttcaccaaag ttatcttcag ccagctgacg gaaagcgatc
    16621 agtgcctctt cagcttctgg ccctttcgca atcaggcgca gcgtatcgtt atagcgaact
    16681 tgtagtaacg caatctggtt aatactctct ggtgtgacgc atttgccgtt tttttccagc
    16741 aacatatcgg cattaaatgt cgataaggta taaaccagcc gggaggccgg acgtacatgc
    16801 aggccgttac ggttttttat gaccaccgcc agagaacggg cttcttcatc gtacgcagga
    16861 catgtgtcag agatttcagt gtcggaggac ggtaaaccca gttgttcacg tttggcttcc
    16921 agcgcatgca tagcgtcaaa gataactttg tcgatatccg ctcccgaggc cgcgctgacc
    16981 gttgctgcca gtgtaccttc gaccaacggc gcagcacaca aacgtacttt tgcggcgatc
    17041 ttgggagcca gcaattccag cgcagtttca gcactcaata atgcgctacc catatccatc
    17101 atgaccagca catggtcggc atcagcaaca gattcgatgg cctccatcac tttgacggca
    17161 tcggtaccaa taggattttg tggatcgtca attcccgcgg caatggcgat tttacaacta
    17221 tcactcatta acatctgacg ggctaattca ccgacacctt ctcccagtcg gctgctatgt
    17281 gaaactatga ccaggtttac catcaccaat tccttactct tttgcggcta acgccaacat
    17341 ttgcatcata aacatcaccg aggtcgcgcc gggatcctgg tgaccaatac tgcgttcacc
    17401 gagataactg gcgcggcctt tgcgggcttg catcgtaatc gtactttgtg cagcggattc
    17461 ggcgatgcta ctggcagctt cgagcgccac cggaacagag agattttgct cgctggactg
    17521 acgtaacgat tccaccaccg gcacccacac atcacacatg gttttatcgc caggttcggc
    17581 tttcccgcga ctgattacgc cgtccgcgcc atcgcggaac atctgataaa gctcttccag
    17641 tgtcaggctt tgccgtgcct gggtcgcctg tgcggcgcgg ataaagaagg taccgaacag
    17701 cggaccactg gcaccaccga cgctggaaag cagcgtcata ccggtattct tgagaatgaa
    17761 accgatatct ttatctgcga tagcagggag tttttccacc actttgctaa agcctcggtt
    17821 catatttagc ccgtggtcag cgtcgccaat ttcgcgatcc agtccggtaa gatactcgct
    17881 ctcggtgctg aaaatatcgc cacaacgagt gagccagtta acaatttgag ttctgctcag
    17941 tgacattgct ttctcctaat ttaccccagt taagggccgg ggtgtggacc ggggcgtccc
    18001 agagtgccag cgtttcgtca tcaactttca gtaaggtgat tgagaaaccg gtcatatcca
    18061 gtgaggtgca gtacgcgcca attaaattac gttcgatagt caatcccgct tgctggcaac
    18121 gtgtggtcag gcggttatag acgccgtaca gctcagaaag cggagttgcg ccaagattgt
    18181 taaccagcgc aatcacccga tcgccagact ggagcggttg tttggtttgt tgttcttcct
    18241 gccaactgcc ttgttgataa tcccagaaac gtaaagtgcg atggtatgag ccatttccca
    18301 gcagggtgtc gaacatttca tcgacggttt gatcaaggga agagaaggag cggcggtcaa
    18361 tacccggctc accatgaatg ccgacgccaa actccatctc attatccgcc agggtaaaag
    18421 aaggtttgcc cgcggcagga acggtacagg caccgagagc gatacctatt gagtggcctt
    18481 gattattcaa cttacgcccc agttccgcac aggcgtccag tgagtcgcca cgctccgccg
    18541 ctgcgcctac gagtttttca attaatacgg tgttggcaac gccgcgtcgc ccggcagtat
    18601 aaagactgtc ttttaccgca acgtcgtcat caatgaccac agtggtcact tttacgccgc
    18661 tatcgtgcag taactcggtc gctgtttcaa agttaagaat atcgccggtg taatttttga
    18721 taatcaacag tacaccttcg ccgccatcaa cttgcatggc gcattcaaag attttatcgg
    18781 gcgtcggtga ggtgaaaatt tcgcccggac aggcccccga aagcatcccc tgaccgatat
    18841 acccacagtg catcggctcg tgtccgctgc cgccacccga cagcagggcg acttttcctg
    18901 caacaggggc atcagctcgg gtgacataca ccggatcctg atgcagtgtc agcgatggat
    18961 gcgctttcgc cagtcctgcc agttgttcgt ccagtacgtc ttgcacatca ttgatcaatt
    19021 ttttcattat tttgctccag caattacggt agggcatgga tgatgttcaa cgacacggcg
    19081 accactgact gccgatgaat ccattgtgca tcaggcaagg gaaaagaaaa ttagcccaaa
    19141 ttatgtttca tagtgaaaca tatgctttaa tgaatgttcc atattgaaac ttttacgtgt
    19201 attaatactt aaaattgcga gccggaacac cttttgtcat aagggatgcg ggatatgagt
    19261 ggcgctttta acaacgatgg tcggggcata tctcccttaa ttgcaacctc ctgggagcga
    19321 tgcaataagc tgatgaaacg ggagacatgg agcgtaccac atcaggccca gggcgtgaca
    19381 tttgcttcta tttatcggcg taagaaagcg atgctgacgc tcgggcaggc tgcgctggaa
    19441 gatgcctggg aatatatggc accgcgagag tgtgcgctgt ttatcctcga tgaaaccgcc
    19501 tgcattctca gccgtaatgg cgatccgcaa accttgcagc agctaagtgc acttggattc
    19561 catgacggca cgtattgcgc cgagggaatt attggtactt gtgcgctatc gttagcggct
    19621 atctctggtc aggccgtgaa aacgatggcc gatcaacatt tcaaacaggc actctggaac
    19681 tgggcctttt gtgcaacgcc gttgtttgac agcaagggcc gattgacggg aacaatagcg
    19741 ctggcgtgtc cggttgagca aactaccgca gctgatttgc cgttgacgtt ggcaatcgcc
    19801 cgcgaggtcg gaaatttact gctgacggac agtttgctcg ctgaaactaa ccgtcattta
    19861 aatcaactta atgccctgtt agaaagtatg gatgatggcg tgattagctg ggacgagcag
    19921 gggaatttgc aatttatcaa tgcccaggcg gcgcgggtct tgcgccttga cgcgacggca
    19981 agtcagggac gggcaatcac tgaactctta acgttacccg ccgtattgca acaagcaata
    20041 aaacaggcac atccgctcaa acacgtagaa gcaacctttg aaagtcagca tcagtttatt
    20101 gatgcggtga taacccttaa accgataata gaaacgcagg gaaccagctt tattttgttg
    20161 ctccatcctg tggaacagat gcggcagttg atgaccagtc aattaggaaa agtcagccat
    20221 acctttgctc atatgccaca ggacgatccg caaacccgcc gcttgattca ttttggtcgc
    20281 caggcggcgc gcagtagctt tcctgtcctg ctttgtggag aagagggcgt gggcaaggca
    20341 ctgctaagtc aggcaattca taatgaaagc gagcgtgctg caggtcctta tatcgccgtc
    20401 aattgtgagt tatatggtga tgcggcactg gcggaagaat ttattggtgg cgatcgtacg
    20461 gacaatgaaa atggccgtct gagtcggctg gaactggcgc acggcggcac gctgtttctt
    20521 gaaaagattg aatatctggc ggtggagtta cagtctgctt ttcttcaggt tatcaagcag
    20581 ggtgttatca cgcgactgga tgcgcggcgt ttaataccaa ttgatgtcaa agtgattgca
    20641 acaacgaccg cggacctcgc aatgctggtg gaacaaaatc gttttagtcg ccagctgtat
    20701 tacgcgctgc atgcatttga aattaccatc ccgcctctgc gtatgcggcg tggcagcatt
    20761 ccggcgctgg tgaataacaa attacgcagt cttgaaaaac gcttctctac gcggctgaaa
    20821 attgatgacg atgccctcgc tcgcctggtt tcttgtgcat ggccaggcaa cgattttgaa
    20881 ctttacagcg tcatcgagaa tcttgctctg agtagtgata atgggcgcat tcgcgtcagt
    20941 gatttgccgg aacatctgtt taccgagcag gcaacagatg atgtcagcgc cacccgcctt
    21001 tccaccagtc tgtcatttgc ggaagttgaa aaagaggcaa ttattaacgc agcccaggtc
    21061 acaggcggtc gcattcagga aatgtcggct ttacttggaa tcggccgcac tacgctgtgg
    21121 cggaaaatga agcaacatgg cattgatgca gggcagttta agcgccgggt atgaaagaca
    21181 gaaacgattt ctaatacatc agagtgatct atatttcatt ccggcgcacg ctaacattta
    21241 tcagcatcgt ttaagggctt gtctatcacg cacttaaaag ctgaagcgat atcctccgtt
    21301 gacttgctt
//
LOCUS       DAJTNA010000059        21035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTNA010000059 DAJTNA010000000
VERSION     DAJTNA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21035
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.060"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..218
                     /locus_tag="OO161_001851"
     CDS             <1..218
                     /locus_tag="OO161_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462942.1"
                     /translation="DFGESEWIHFTGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVT
                     LIRRYGVSLIHLDASAECLPGLPTFDW"
     gene            230..427
                     /locus_tag="OO161_001852"
     CDS             230..427
                     /locus_tag="OO161_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462943.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            512..1072
                     /locus_tag="OO161_001853"
                     /pseudo
     CDS             512..1072
                     /locus_tag="OO161_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            1144..2148
                     /locus_tag="OO161_001854"
     CDS             1144..2148
                     /locus_tag="OO161_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5462944.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            2432..2710
                     /locus_tag="OO161_001855"
                     /pseudo
     CDS             2432..2710
                     /locus_tag="OO161_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(2860..2947)
                     /locus_tag="OO161_001856"
     tRNA            complement(2860..2947)
                     /locus_tag="OO161_001856"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2911..2913),aa:Ser,seq:gga)
     gene            complement(3201..3419)
                     /gene="infA"
                     /locus_tag="OO161_001857"
     CDS             complement(3201..3419)
                     /gene="infA"
                     /locus_tag="OO161_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5462945.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(3704..4408)
                     /gene="aat"
                     /locus_tag="OO161_001858"
     CDS             complement(3704..4408)
                     /gene="aat"
                     /locus_tag="OO161_001858"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5462946.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(4450..6171)
                     /gene="cydC"
                     /locus_tag="OO161_001859"
     CDS             complement(4450..6171)
                     /gene="cydC"
                     /locus_tag="OO161_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5462947.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(6172..7938)
                     /gene="cydD"
                     /locus_tag="OO161_001860"
     CDS             complement(6172..7938)
                     /gene="cydD"
                     /locus_tag="OO161_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5462948.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(8061..9026)
                     /gene="trxB"
                     /locus_tag="OO161_001861"
     CDS             complement(8061..9026)
                     /gene="trxB"
                     /locus_tag="OO161_001861"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5462949.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            9571..10065
                     /gene="lrp"
                     /locus_tag="OO161_001862"
     CDS             9571..10065
                     /gene="lrp"
                     /locus_tag="OO161_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5462950.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVSDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            10200..14228
                     /locus_tag="OO161_001863"
     CDS             10200..14228
                     /locus_tag="OO161_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5462951.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QAQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQPVAPQQQYQQPQQPV
                     APQQQYQQPQQQVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            14383..14994
                     /gene="lolA"
                     /locus_tag="OO161_001864"
     CDS             14383..14994
                     /gene="lolA"
                     /locus_tag="OO161_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5462952.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            15005..16348
                     /gene="rarA"
                     /locus_tag="OO161_001865"
                     /gene_synonym="mgsA"
     CDS             15005..16348
                     /gene="rarA"
                     /locus_tag="OO161_001865"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5462953.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAISELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            16439..17731
                     /gene="serS"
                     /locus_tag="OO161_001866"
     CDS             16439..17731
                     /gene="serS"
                     /locus_tag="OO161_001866"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5462954.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            17969..20413
                     /gene="dmsA"
                     /locus_tag="OO161_001867"
     CDS             17969..20413
                     /gene="dmsA"
                     /locus_tag="OO161_001867"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCR5462955.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGSGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGDVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            20424..>21035
                     /gene="dmsB"
                     /locus_tag="OO161_001868"
     CDS             20424..>21035
                     /gene="dmsB"
                     /locus_tag="OO161_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5462956.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKE"
BASE COUNT         5404 a         5278 c         5505 g         4848 t
ORIGIN      
        1 gtgacttcgg tgaatcagaa tggattcatt ttacaggtag cggatatctg ttacgtaccg
       61 atgcgtggtc atacccggtt ctgcggctta aacgcctggg gctgtcaaaa acgttccgtc
      121 gtctggtcgt cacactcatc cggcgttatg gcgtcagtct cattcatctg gatgccagcg
      181 ctgaatgcct gccgggttta cccactttcg actggtaacc aggaacaaca tgaaatcatt
      241 aaccacggaa acagcgctgg atattctgat tgcgtggctg caggacaata tcgactgcgg
      301 atcgggaatt atctttgaca acgatgagga taaaacggat tcagcagcac tgttgccctg
      361 tatcgaacag gcccgggagg atgtccgtac cctgcgtcat ctgcagcttc tgcaccagaa
      421 ccggtgaagc tcactcacca gacttcattc ctctgacgcc agcctgcaca gggctggcgt
      481 tttcatttat ctgcaaaaag gaatatcgat tatgtcagat atcacaatct cccgtccgga
      541 agtggtcaac gggcatacgg acgttatctg ctcaaccagc attcgccaca ttctggctgt
      601 acgaaagagt acactgttgc aaatcgacac acttatccga caactggctg aaatctcggt
      661 gctgacggaa agcatcggag gtaaaactgc cccggactgg gccatgaaac aggattttcg
      721 ctgcggctgc tggctgatgg agaaacccga aacagcaatg aaagccatta cccggaatct
      781 tgatcgcgaa atctggcgtg acctgatgca acgttccggg atgctgtcat taatggatac
      841 gcaggcccgt gatacatggt accggtcact ggagtacgat aattttccgg aaatcagcga
      901 agcgaacatt ctgagcacat ttgaacaact gcaccagaat aaggatgagg tgtttgagcg
      961 aggggtgatc aacgtcttca gagggctgaa ctggaattac aaaaccaatt gcccctgtaa
     1021 atttggcagt aaaattatcg tcaacaatct ggtgagatgg gaccggtggg gataatgata
     1081 tggtcacccc caccttaaca gcagtacgct gttaaggtaa atccctttaa cggagagacc
     1141 atcatgcaga acatcacact tattggtatc gatctcggaa agcactcctt ccacattcat
     1201 tgtcaagaca agtctggtaa ggctcttctg cgtaaaaaat ttacccgtac taaactgatg
     1261 cagtttctgg cgacatgtcc gccctcagtt gttgtaatgg aagcctgtgc gggtgcacac
     1321 tttatggctc gctgtatctc tgattttggg catgaaacta agctgatttc tccacagttc
     1381 gttcgcccgt ttgtcaaaag taataagaat gattttgttg atgcagaagc catatgcgaa
     1441 gcggcttcac gaccatcaat gcgttttgtc cagccaagaa ctgagaccca gcaggccatg
     1501 agggcgttac accgggtcag agaatcgctc atcagagata aggtgaaaac aaccaaccag
     1561 atccacggtt tcttgctgga atttggaatc agcctgccga taggtgatgc tgtaataaaa
     1621 cggttgtctc tcgtgttggc tgaacacgaa atccccgaat atctgagccg ccttttggtg
     1681 aggttgcata cccactactt ttaccttgtt gagcaaatag cagagctgga atcagaatta
     1741 agtcagtcaa tcaacgctga tgataccgct cagcgcatta tgacaattcc tggtgttggt
     1801 ccgattactg ccagcttact atcgtcgcaa cttggtgacg ggaaacagtt ctcatgcagc
     1861 agggattttg cagcttccac aggacttgtt ccccggcagt atagcactgg aggaaaatcg
     1921 actctgttgg gtatcagtaa gcgaggtgat aaaaatttac gccggttact ggttcagtgt
     1981 gcccgctcat tcataatgcg acttgaacgc aagcatggaa aacttgctga gtgggttcgg
     2041 gagcaactca acaagaagca ttcgaatgtg gttacctgcg ctctggcaaa taaactggct
     2101 cgaattgcct gggcaatcac tactcagaaa aaagaatatc aggcctgata tagatgctga
     2161 tataactcaa ttagtttatt ttaaacaata agatacaagt tacctttctg gttttgcgaa
     2221 gactgattat tgatgacatg aacggcaaat cgacctgatt aaaaacctaa cagatgaaat
     2281 ggctcactga agccgagggg atttttagga ttatcaggtg cggaactcat cggggcgcgg
     2341 gcatccttgc ccacagagac gccggataga tttaagcaag ccaaccatta atcaaaaatc
     2401 agtgttgcaa aaacgggggt gaccatagat tcatcttatc accggacaac aggctgaccg
     2461 actcgccgat ctggaaagaa tgttgcacct gttcagcggt aaaccaatcc ccgacaaccg
     2521 gggaaacatc acccttcgtc tggatgatca cattcagtct gttcagggta aagagagcta
     2581 tgaagatgag atgttcagca tcagatactt taagaagggc tccgcgcata tcacgttcag
     2641 aaaaccagaa ctggttgaca gactgaatga tattatcgca aaacactatc ctgatatgct
     2701 ggcagtatag ctttgccagt atgctcaaag atcggaaatt gtccgataag atatgatcgc
     2761 ggggcatata aaccggtttc agatggcata cccgatggca tactgtctgg aaggaaaaat
     2821 aaaaaagcgc atgttaaaca ataacatgca cttataatct ggcggtgagg gggggattcg
     2881 aacccccgat acgttgccgt atacacactt tccaggcgtg ctccttcagc cactcggaca
     2941 cctcaccaaa ttgttttgct accaaacctc atgggtggca acggggcgct actataggga
     3001 gttggagtaa aacggtcaag aagaatttta atgataatta ttgtttgctc atactgtaaa
     3061 caagttgtgc agtatatcta catcgagaca agttacggac ttatacttcc aaagtacttc
     3121 atacatatca caaaataaaa aggccggtta aaccgacctt ttactcgttc tttctcttcg
     3181 cccatcaggc ggtaaaacaa tcagcgacta cggaagacaa tgcggccttt gctcaggtcg
     3241 tacggggtca gttcaacagt cactttgtcg cccgtcagga tgcggatgta gtttttgcgc
     3301 attttaccgg agatgtgtgc agtaaccacg tgaccgtttt ctaactctac gcggaacatg
     3361 gtattaggca acgtttcaag aacggtacct tgcatttcaa tattgtcttc tttggccatc
     3421 taatcctctg gggtatcact accgtaattt gaaccggcaa gataatgccg aagttctgta
     3481 aataagtaaa gatttgcgcg ctaaatcgca acaaacaggt tcggcacatt actccgaaaa
     3541 cacacggcta agccgcacca aaagcgcaac gtataaggga gcggtgagat aaacgatggg
     3601 cgttacctga cgcgaaaaat tccttatcgg cagcggggta atgagcgtaa ccaactctgc
     3661 gaccgcaatt ataacactct ggggagaaat gtgccgaaaa cattcattct tgtggtgaaa
     3721 acaagcatcg tggtacccag aaattattcg gcaatcgtcc gaggcgcatt tgattgagat
     3781 aattaaggta atcccggcgg ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa
     3841 ggacctggca gtcgataagc ttaccgccat gaccgataaa ttcctcacag aataccagaa
     3901 gcgccgtttt agacgcattt tccatccggc tgaacatgga ctcgccacaa aatagcgttc
     3961 cctgggccac gccgtacata ccgccgacaa gctcatcttc acgccagact tcaatggagt
     4021 gggcatggcc gagttcgtga aggcgatggt aggcttcgac cacgccacgc gtgatccagg
     4081 ttccttcttc gcgatcgctg gcacagcctt caatgacctg accaaaagcg taattcatcg
     4141 tgacacgata gggcgagcgt ttatgaaatc gcttcatact acggctgata tgcagtgatt
     4201 ctggccatag caccgcgcgg ggatcgggcg accaccagag gatggggtcg cctggagaaa
     4261 accacggaaa aataccacgc tggtaagcca ttaacaggcg cgcagggcta agatcgcccc
     4321 caagtgccag caggccgtta ggctcacgta atgcgccttc cggggaaggg aaggctattg
     4381 aatggcgaga aagctgaacc aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc
     4441 aataatagct tacaaaccct gcttgaactg gtaataacgc ccctgtctgg caagcagctc
     4501 tgcgtgagta ccttgctcaa taatttgccc gttgtccatc actattattt gttggaaacg
     4561 agagagtccg cgaagtcgat gggtgaccat taacaccgtt ttctcacgca tcatttctgc
     4621 aagcaattca aggatctggc tttcggttgt ggcatctaag ccttcggtag gttcatccag
     4681 caacaccagt ggcgcatcat gtaacagcgc acgggcgata gccagacggc gcagttcacc
     4741 accggagagc tggcgtccgc cttcacctaa ccaactgttg agacctgcat cctcgagcag
     4801 cttctccaga ccaacgcgac gcaagctctc cgccagagcc tcatcactac tgccaggcga
     4861 ggcgagtaac agattatcac gcagcgtggc gctaaacaga tgcactcgct gaggaacaac
     4921 gctgatggtc tgtcgtagag ccgcttcatt caggctggct atggggctat cgttaagcaa
     4981 aatctcgccc tgttgcgggt cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc
     5041 gcatccggtt cgcccgagaa tcgcaatatg ttccccggcg tttacctgaa gagaaatccc
     5101 tttaagtgcc tgttgggatt gctccggata agtgaactga acatcccgta atgtcagcga
     5161 aacgcgatcg gcaacacgag tttgggtatc agggaaggtg acttccggtt tttgatccgt
     5221 taaatcagtg atccgaacgg cagaggcaat gacttgcccc agatgctgaa atgcacccgt
     5281 tactggtgcc agtgcttcaa acgcggctaa cgcgcagaag acaaacaggg caattaacgc
     5341 gccgggttga gcattgccgc caacgccgcc agacgccatc cacagcatca ggatcaccgc
     5401 taacgcgcca atgagcagca ttatcgcttg cgacaatgcg gtcagttcag attgacggcg
     5461 ttgcgcttcc agccattgaa tttctgtatt ctctagttgc gtgcgataac gatcgctggc
     5521 accaaaaatg gtcagctcag cttgcccttg cagccaggcc gtcagttgtt ggcgatactg
     5581 tccgcgaaga tgagtcagat tttgcccggt gctttttccc gcacgataaa acagcggtgg
     5641 catcaggaaa agcgtcagta acataatgcc gcccagcgta aaggcgaggg tgaaatcaag
     5701 gaaacttaac ccgattgtca ccatcataat caccacaaaa gcgcccacca gcggcgagat
     5761 aacgcgcagg taaagatgat cgagcgtatc aacatccgcc accacgcgat tgagcaattc
     5821 gccctgacga tagcgcgcca gtccggcagg ggagaggggc aacaatttgc tgaaggtgta
     5881 aatgcgcaga tgctgcaaca cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata
     5941 acgcccggca gtacgggtga ttgcggcacc acgcacgccc gcagcgggta gcatatagtt
     6001 gaagctgtac agtccggcaa cccccgcaac cgctgaggcc gagaggaacc agccggaaag
     6061 tgtcaacagt ccgatactgg cgagcagcgt cacaattgcc agcacaatac caagacttaa
     6121 catccattta tgacgtttat acagtgctag atagggtagc aaagcgcgca tttaaatctc
     6181 ctcctgacga tgggccagta atgtggcgaa tgggccacca gccacactta attccgcgta
     6241 acgtccttgc tcaataatcc ggccatcctg cataacccaa atgacatccc agtcagcaag
     6301 atcttctaac tggtgggtga ccattaacgt tgtctggcgc agagaggcgg cattcagcgc
     6361 ctccattacg cgctgttcac tgtgagcatc aaggctggca gcgggttcat ccaacagtaa
     6421 tagcgaacag ggatttagta acgcacgggc caccgccacg cgctgcgcct gccccacgga
     6481 aaggcgggca gcctggtcgc caacaggcgt atcaacgcct tgtggcagga gcggtagaaa
     6541 ctcgctgacc caggcgttat ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg
     6601 cgccagtagt acgttatccc gtaatgttgc tgccggtaat tgtggatttt gcccaaccca
     6661 ggagagatgt ttacgccatg attctggtga taaatcgcgt aattctatcc cgttgattcg
     6721 tagcgatccc tgatatgaga gaaaaccaga aagcgcgttc aacagtgaac ttttacctga
     6781 accgctgcga ccaaccaaca cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc
     6841 ggccagcgtt ttaccttccg gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg
     6901 atcggtcgat gctaattcca cctcgccacg ctgcggatgg gcgagcgggg tttccataaa
     6961 cgttttcaga ctgtcagctg caccaacagc ctgggcttta gcatgataaa acgtaccgag
     7021 atcgcgtaat ggctggaaaa actctggcgc aaggatcagg gccagaaaac ccgcagccag
     7081 cgtcacgcca gtatcgtagt gaccaaaatc cagctcgccg agataggaaa aaccaaagta
     7141 gaccgccacc agagcaattg acagcgaggt aaaaaattcg agaatgccgg aggataaaaa
     7201 tgccagccgt agcacttcca ttgtccgttg gcggaaatct tccgaagcag aacgaatact
     7261 ttcaatttca gcttcaccac gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc
     7321 gaggaaatgc ccacttaagc gagcaagagc gagaaagtta cgtcggttag catcggcagc
     7381 ccccattcca accagcgcca taaacaacgg aattaacggt gcagtgccca gcagaatgag
     7441 cgccgcagcc cagtttgagg ggaagattgc caccacaatc agcaacggca ccgacactgc
     7501 cagcgccatt tgcggcaggt agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag
     7561 taccagcgtc gcccagctcc ccgcaggttt accctgaatc cacgctggcc ctgcttgttg
     7621 cagacggtcg agaacctgcc ggcggatggc aaagcggata tgctgcccgg cgtgataacc
     7681 cacccgttcg cgtaaccaga ccacccatgc gcgcagtaca aaggtcagaa ccagtaacgt
     7741 aaagggaagc agcagggctt cacggggaat attctccata atcatatgtt gcagaattcg
     7801 cgccatgaac caggcctggg caatgatcaa tatgccgctc acaaagccca gcagacgaga
     7861 aatattcagc caacgttggg agatgacgct ttgctgtttt aaccagcggg ttaactcttt
     7921 ttgacgagat ttattcattg cacgcttagc aggtgagtta tcagaattat ttgcagagca
     7981 atgttacaac ggggaaaaaa taaaggcgac ccatagtcgc aaggtgtcgc cttctttact
     8041 tttgttactg atttgtaaaa ttattttgcg tcagctaaac catcgaggta gcgttccgca
     8101 tcaagtgctg ccatgcagcc tgtaccggcc gaagtaatgg cctgacgata aatatgatcc
     8161 atcacgtcac ccgcggcaaa gacgccagga atgctggtct gggtggcatt accatgaata
     8221 cccgactgta ctttgatgta gccgttttcc agttccagct gcccttcgaa aatggcagta
     8281 ttcgggctgt gaccgatagc aacaaacaga ccggcaacgt cgagtgattc gatgttatcg
     8341 ctgttttgcg tatcgcgcag acgaacgcca gtgacaccca tttgatcgcc ggtcacttct
     8401 tccagcgtcc ggttggtgtg cagaatgatg ttgccgttct ccactttatc catcagacgc
     8461 ttaatgagga ttttttccgc gcggaaaccg tcacggcggt gaatcagatg tacttccgaa
     8521 gcgatgttag acagatacaa cgcctcttca accgcggtat tgccgccgcc gatgaccgca
     8581 actttctggt tgcgatagaa gaaaccgtcg caggttgcac aagcagaaac cccacggcct
     8641 ttaaaggctt cttcagaggg caggccgaga tagcgtgcag aagctccggt ggcaataatc
     8701 agcgcgtcgc aagtgtattc gccgttatcg ccattcagac ggaacggacg gttttgcaga
     8761 tccaccttgt tgatatgatc aaaaatgatt tcagtttcaa atttggtggc atgttcgtgc
     8821 atgcgctcca ttaataacgg accggtcaga tcgtttggat cgccaggcca gttttccact
     8881 tctgtggtgg tggtcagttg gccgcctttt tccatgccgg taatcagcac aggttgcagg
     8941 ttggcgcgcg ccgcatagac agcagcggtg tatcccgccg ggcctgaacc caggataagc
     9001 agtttactgt gtttggtcgt gcccatgaga tccccatagt tgttggcaga caatgggcag
     9061 gattgtaggg aatttactga cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc
     9121 aatcggcagc atcgataagc aggtcaaatt ctcccgtcat tatcacctct gctacttaaa
     9181 tttcccgctt tataagctga ttaaatgatg aataaacgcc cctgttaatg aatatctggc
     9241 atgttgtact aaaaatcgat gttttgcttt gacaatcccc tagtgttttg cgaaaacatt
     9301 cgaggaagaa aaaaaacagt attcttatat gcgcataacc atgcatgtaa ataccatgtt
     9361 taccgtgcta gtgaaatcta cgtatggcgt ggacagacgc cattcgtgat gtcgatagct
     9421 gccacaaggc aacggtcttc tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg
     9481 atttcggtct atcgtgacgg gtagcgactc tgagcagtga tgtttcaggg tcagacagga
     9541 gtagggaagg aatacagaga gacaataata atggtagata gcaagaagcg ccctggcaaa
     9601 gatctcgacc gtatcgatcg taacattctt aatgagttgc aaaaggatgg gcgtatttct
     9661 aacgtcgagc tttctaaacg tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt
     9721 cggctggaaa gacaagggtt tattcagggc tatacggcgc tgcttaaccc ccattatctg
     9781 gatgcatcac ttctggtatt cgttgagatt actctgaatc gtggcgcacc ggatgtgttt
     9841 gaacaattca ataccgctgt acaaaaactt gaagaaattc aggagtgtca tttagtatct
     9901 ggtgatttcg actacctgtt gaaaacacgc gtgtcggata tgtcagctta ccgtaagttg
     9961 ctgggggaaa ccctgctgcg tctgcctggc gtcaatgaca cacggacata cgttgttatg
    10021 gaagaagtca agcagagtaa tcgtctggtt attaagacgc gctaacacgg aacaggtgca
    10081 aaatcggcgt attttgatta cactcctgtt aatccataca gcaacagtac tggggtaacc
    10141 tggtactgtt gtccgtttta gcatcgggca ggaaaagcct gtaacctgga gagcctttct
    10201 tgagccagga atacactgaa gacaaagaag tcacattgac aaagttaagt agcggccgtc
    10261 gccttctgga agcgttgctg atccttattg tcctgtttgc cgtctggttg atggctgcct
    10321 tactaagctt taacccttcg gaccccagct ggtcgcaaac ggcctggcat gaacctatcc
    10381 ataatttagg tgggatgccc ggtgcgtggc tggcagatac gctgttcttt atttttggcg
    10441 tgatggctta caccattccc gtcattattg tcggcggttg ttggtttgcc tggcgtcatc
    10501 agtccagtga cgaatacatt gattattttg ccgtttcgtt acgcatcatt ggcgttttgg
    10561 cgctcatcct tacctcctgt ggtctggcgg caatcaacgc tgacgatatc tggtattttg
    10621 cctccggtgg cgtcattggc agcttactaa gcactacgtt acaaccactg ctacacagta
    10681 gcgggggaac tattgcgctg ctctgcgttt gggctgcggg cctgacgctg ttcaccggtt
    10741 ggtcatgggt gaccattgct gaaaaactcg gcggctggat tttaaacatt ctcaccttcg
    10801 ccagtaatcg tacccgtcgc gatgatacct gggtcgatga agatgaatat gaagatgacg
    10861 aagagtatga agatgaaaac cacggcaaac agcatgaatc acgccgtgcc cgtattcttc
    10921 gcggcgcgct agcgcgtcgt aaacggttgg cggaaaaatt cattaatccg atggggcggc
    10981 aaacagacgc agcgttgttc tccggtaagc ggatggatga tgaagaagag attatctaca
    11041 ctgcacgcgg tgtggctgcc gacccggacg acgtcctatt ttcgggcaat cgtgcaacgc
    11101 agccagaata tgacgaatac gatccattat taaacggtgc gccaattacc gaacctgttg
    11161 ctgtagcagc tgctgctacc acggcgacac aaagctgggc tgcgccggtt gaacctgtga
    11221 cccagacgcc gcctgttact tctgttgatg ttccaccttc gcaacctaca gtagcctggc
    11281 agcctgtacc gggtccacaa acgggagagc cggttattgc tcctgcaccg gaaggttacc
    11341 cacagcagcc acaatatgcg cagcctgcag tgcaatataa tgagccgctg caacaacctg
    11401 tacagccaca gcagccgtat tatgcgcctg cagctgaaca acctgtgcaa caaccttact
    11461 acgctcctgc ggcggaacaa cctgttcagc aaccgtatta cgcaactgcg ccggaacagc
    11521 ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc ggtggcaggt aacgcctggc
    11581 aagccgaaga gcagcaatcc acttttgctc cacagtctac ataccagact gagcaaactt
    11641 atcagcagcc agccgctcag gagccgttgt accaacagcc gcaacccgtt gaacagcagc
    11701 ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc cgcgcgtccg ccgctttact
    11761 attttgaaga agtggaagag aagcgagccc gtgaacgtga acaacttgcg gcctggtatc
    11821 aaccgattcc agaaccggtt aaagaaccag aaccgatcaa atcttcgttg aaagcgcctt
    11881 ctgttgcagc agtacctcca gtagaagccg ctgccgctgt ttctccgctg gcatctggcg
    11941 tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc
    12001 tggcaaatag cggtggaccg cgtccgcaag tcaaagaggg aattggcccg cagttgccac
    12061 gaccgaaacg tatccgcgtg ccaactcgtc gtgaactggc gtcttacggt attaagctgc
    12121 cctcacagcg tgcggcggaa gagaaagccc gtgaagcccg gcgcaatcag tatgattctg
    12181 gcgatcagta taacgatgat gaaatcgatg cgatgcagca ggatgaactg gcacgtcagt
    12241 tcgcccaggc acagcagcaa cgctatggcg aacagtatca acatgatgtg cccgtaaacg
    12301 cagaagatgc agatgctgcg gcagaggctg aactggctcg tcagtttgcg caaactcaac
    12361 aacaacgtta ttccggcgaa caaccggctg gggcgaatcc gttctcgctg gatgattttg
    12421 aattttcgcc aatgaaagcg ttgctggatg atggtccaca cgaaccgttg tttacgccaa
    12481 ttgttgaacc tgtacagcag ccgcaacaac tggttgcacc gcagcagcag tatcagcagc
    12541 cacaacagcc ggttgcgcca cagcagcaat atcagcagcc gcaacagccg gttgcgccgc
    12601 agcagcagta tcagcagcca caacagcagg ttgcgccgca gccacaatat cagcagccgc
    12661 aacaaccggt tgcaccgcag cagcaggata ccctgcttca tccgctgttg atgcgtaatg
    12721 gcgacagccg tccgttgcat aaaccgacga cgccgctgcc ttctctggat ttgctgacac
    12781 cgccgccgag cgaagtggag ccggtagata cctttgcgct tgaacaaatg gctcgcctgg
    12841 tggaagcgcg tctggctgat ttccgtatta aagccgatgt cgtcaattac tctccggggc
    12901 cggttatcac tcgctttgaa cttaacctgg ctcctggcgt aaaagcggcg cgcatttcta
    12961 acttgtcacg ggaccttgcc cgttcacttt cgacggtggc ggtgcgtgtc gttgaagtta
    13021 ttcctggcaa accctatgta ggtctggagt taccaaataa aaaacgacaa accgtttatc
    13081 tgcgcgaagt tttggataac gccaaattcc gcgataatcc gtcgccatta accgtggtgc
    13141 tgggtaaaga tatcgccggt gagccggtgg ttgccgatct ggcgaaaatg ccgcacttgt
    13201 tggttgcggg gactaccggt tccggtaaat ctgtcggtgt gaacgcgatg atcctgagca
    13261 tgctttataa agcacagcca gaagatgtgc gtttcatcat gatcgacccg aaaatgctgg
    13321 agctttcggt ttatgaaggc attccgcatc tgttaacgga agtcgttact gatatgaaag
    13381 atgccgccaa cgcgctgcgc tggtgtgtta acgagatgga gcgtcgttat aaactgatgt
    13441 ctgcgctggg tgtgcgtaat ctggcgggtt ataacgaaaa aattgctgaa gccgatcgca
    13501 tgatgcgtcc gattccagac ccgtactgga agccgggtga cagtatggat gcccagcatc
    13561 cggtgctgaa aaaagaacca tacattgtgg tgttggttga cgaatttgcc gacctgatga
    13621 tgacggtagg taaaaaagtg gaagagctga tagcacgtct ggcgcaaaaa gcccgtgccg
    13681 cgggtatcca cctcgtactg gcaactcagc gtccatcggt tgatgttatt actggtctga
    13741 ttaaagcgaa tattccgacc cgtatcgcct ttaccgtatc cagtaagatt gactcacgta
    13801 ccattcttga tcaggctggc gcggaatcac tgctggggat gggggatatg ctctactctg
    13861 ggccgaactc cacgttgccg gtacgtgtcc atggtgcttt tgttcgcgat caggaagttc
    13921 atgccgtggt gcaggactgg aaagcgcgtg gtcgcccaca gtatgttgat ggcatcactt
    13981 ccgacagtga aagcgaaggt ggcgcgggcg gtttcgatgg cgctgaagaa ctggatccgt
    14041 tgttcgatca ggcggtgcag tttgtcactg aaaaacgcaa agcgtcaatt tctggcgtac
    14101 agcgtcagtt ccgcattggt tataaccgtg cagcgcgtat tatcgaacag atggaagcgc
    14161 aggggattgt cagcgaacag gggcacaacg gtaatcgtga agtgctggcc ccaccgccgt
    14221 ttgactaatt aatgcattgc cggataaggc gcggtagcgt cgcatccggc actctatcaa
    14281 ctgaaaattc agtattttct tttttcctca agctgattat tagcctggaa tagagagtag
    14341 agggaactcc cgatcgggag tgacgtaatt tgaggaataa tgatgaaaaa aattgccatc
    14401 acctgtgcat tactctcaag cttagtagca agcagcgttt gggctgatgc cgcaagcgat
    14461 ctgaaaagcc gcctggataa agtcagcagc ttccacgcca gcttcacaca aaaagtgact
    14521 gacggtagcg gcgcggcggt gcaggaaggt cagggcgatc tgtgggtgaa acgtccaaac
    14581 ttattcaact ggcatatgac acaacctgat gaaagcattc tggtttctga cggtaaaaca
    14641 ctgtggttct ataacccgtt cgttgagcaa gctacggcaa cttggctgaa agatgccacc
    14701 ggtaatacgc cgtttatgct gattgcccgc aaccagtcca gcgactggca gcagtacaat
    14761 atcaaacaga atggcgatga ctttgtcctg acgccgaaag ccagcaatgg caatctgaag
    14821 cagttcacca ttaacgtggg acgtgatggc acaatccatc agtttagcgc ggtggagcag
    14881 gacgatcagc gcagcagtta tcaactgaaa tcccagcaaa atggggctgt ggatgcagcg
    14941 aaatttacct tcaccccgcc gcaaggcgtc acggtagatg atcaacgtaa gtagaggcac
    15001 ctgagtgagc aatctgtcgc tcgatttttc ggataatact tttcaacctc tggccgcgcg
    15061 tatgcggcca gaaaatttag cacagtatat cggccagcaa catttgctgg ctgcggggaa
    15121 gccgttgccg cgcgctatcg aagccgggca tctgcattct atgatcctct gggggccacc
    15181 gggtaccggc aaaacaactc tcgctgaagt gattgcccgc tatgcgaacg ctgatgtgga
    15241 acgtatttct gccgtcacct ctggcgtgaa agagattcgc gaggcgatcg agcgcgcccg
    15301 gcaaaaccgc aatgcaggtc gccgcactat tctttttgtt gacgaagttc accgtttcaa
    15361 caaaagccag caggatgcat ttctgccaca tattgaagac ggcaccatca cttttattgg
    15421 cgcaaccact gaaaacccgt cgtttgagct taattcggca ctgctttccc gtgcccgtgt
    15481 ctatctgttg aaatccctga gtacagagga tattgagcaa gtactaactc aggcgatgga
    15541 agacaaaacc cgtggctatg gtggtcagga tattgttctg ccagatgaaa cacgacgcgc
    15601 catttctgaa ctggtgaatg gcgacgcgcg ccgggcgtta aatacgctgg aaatgatggc
    15661 ggatatggcc gaagtcgatg atagcggtaa gcgggtcctg aagcctgaat tactgaccga
    15721 aatcgcaggt gaacgtagtg cccgctttga taacaaaggt gatcgctttt acgatctcat
    15781 ttccgcactg cataaatcgg tacgtggtag cgcacccgat gcggcgctgt actggtatgc
    15841 gcgaattatt actgccggtg gcgatccgtt atatgttgcg cgtcgctgtc tggcgattgc
    15901 gtctgaagat gtgggtaatg ctgatccacg agcgatgcag gtggcaattg cagcctggga
    15961 ttgctttact cgcgttggcc cggcggaagg tgaacgcgcc attgctcagg cgattgttta
    16021 cctggcctgc gcgccaaaaa gcaacgctgt ctacactgcg tttaaagctg cgctggcgga
    16081 tgctcgcgaa cgtccggatt atgacgtgcc ggttcatttg cgtaatgcgc cgacgaaatt
    16141 aatgaaggaa atgggctacg ggcaggaata tcgttacgct catgatgaag caaacgctta
    16201 tgctgccggt gaggtttact tcccgcggga aatagcacaa acacgctatt atttcccgac
    16261 aaacaggggc cttgaaggca agattggcga aaagctcgcc tggctggctg aacaggatca
    16321 aaatagcccc ataaaacgct accgttaatg ttatcgttgc ggtaatgttg ttactgtatc
    16381 cctgtggtcg caggctgtgg ccacatctcc catttaattc gataagcaca ggataagcat
    16441 gctcgatccc aatctgctgc gtaatgagcc agacgcagtc gctgaaaaac tggcacgccg
    16501 gggctttaag ctggatgtag ataagctggg cgctctcgaa gagcgtcgta aagtattgca
    16561 ggtcaaaacg gaaaacctgc aagcggagcg taactcccga tcgaaatcca ttggccaggc
    16621 gaaagcgcgc ggggaagata tcgagccttt acgtctggaa gtgaacaaac tgggcgaaga
    16681 gctggatgca gcaaaagccg agctggatgc tttacaggct gaaattcgcg atatcgcgct
    16741 gaccatccct aacctgcctg cagatgaagt gccggtaggt aaagacgaaa atgacaacgt
    16801 tgaagtcagc cgctggggta ccccgcgtga gtttgacttt gaagttcgtg accacgtgac
    16861 gctgggtgaa atgcactctg gcctcgactt tgcagctgca gttaagctga ctggttcccg
    16921 ctttgtggta atgaaagggc agattgctcg catgcaccgc gcactgtcgc agtttatgct
    16981 ggatctgcat accgaacagc atggctacag tgagaactat gttccgtacc tggttaacca
    17041 ggacacgctg tacggtacgg gtcagctgcc gaaatttgct ggcgatctgt tccatactcg
    17101 tccgctggaa gaagaagcag acaccagtaa ctatgcgctg atcccaacgg cagaagttcc
    17161 gctgactaac ctggtgcgcg gtgaaatcat cgatgaagat gatctgccaa ttaagatgac
    17221 cgcccacacc ccatgcttcc gttctgaagc cggttcatat ggtcgtgaca cccgtggtct
    17281 gatccgtatg caccagttcg acaaagttga aatggtgcag atcgtgcgcc cggaagactc
    17341 aatggcggcg ctggaagaga tgactggtca tgcagaaaaa gtcctgcagc tgctgggcct
    17401 gccgtaccgt aaaatcatcc tttgtaccgg cgacatgggc tttggcgctt gcaaaaccta
    17461 tgacctggaa gtatggatcc cggcacagaa cacctaccgt gagatctctt cctgctccaa
    17521 cgtttgggat ttccaggcac gtcgtatgca ggcacgttgc cgcagcaagt cggacaagaa
    17581 aacccgtctg gttcataccc tgaacggttc tggtctggct gttggtcgta cgctggttgc
    17641 agtaatggaa aactatcagc aggctgatgg tcgtattgaa gtaccagaag ttctgcgtcc
    17701 gtatatgaac ggactggaat atattggcta atacccaatt tttctgaatc taaaaagcgc
    17761 ctgcgggcgc tttttttgtc tccctttgat accgaacaat aattactcct cacttacacg
    17821 taatactact ttcgagtgaa aatctaccta tctctttgat tttcaaatta ttctatgtat
    17881 acaagcctat atagcgagct gctatagaaa taattacaca atacggtttg tactggaatc
    17941 aatcgtgagc aagcttgagt gagccattat gaaaacgaaa atccctgatg cggtattggc
    18001 tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg atcggcggcc tggcaatggc
    18061 cagcagcgca ttaacattac cttttagtcg gattgcgcac gctgtcgata gcgccattcc
    18121 aacaaaatca gacgaaaagg ttatctggag cgcctgtaca gttaactgtg gtagtcgctg
    18181 cccgctacgt atgcacgtcg tggacggtga aatcaaatat gtcgaaacgg acaataccgg
    18241 cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc ctgcgtgggc gttccatgcg
    18301 tcgccgtgtc tacaatccgg accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg
    18361 tgaaggcaaa ttcgagcgca ttagctggga agaagcctac gacatcatcg cgaccaatat
    18421 gcagcgcctg atcaaagagt acggcaacga gtctatctat ctgaactatg gcaccggtac
    18481 gctgggcggc accatgaccc gctcctggcc gccgggaaat accctggtcg cgcggctgat
    18541 gaactgctgc ggcggctatc tgaaccatta cggcgactac tcctccgcgc aaattgcgga
    18601 aggtttgaac tatacctacg gcggctgggc agatggcaac agcccgtcgg atatcgaaaa
    18661 cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa acgcgaatga gtggcagtgg
    18721 ggtgacttac tatcttgaac aggcacgcca gaaatctaat gcccgcatga tcatcatcga
    18781 tccgcgctat accgacaccg gtgccgggcg cgaagatgag tggatcccta ttcgtccggg
    18841 aacagatgcc gcactggtta acggtctggc gtacgtcatg atcactgaaa acctggtgga
    18901 tcaggcattc ctcgataaat attgcgttgg ctacgatgag aaaaccctgc cagccagtgc
    18961 gccgaaaaat ggccactata aagcttatat tctgggtgaa gggccagatg gcgtggctaa
    19021 aacaccggaa tgggcctcac aaatcaccgg tgttccggca gacaaaatca tcaaactggc
    19081 tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag ggatggggcc cgcagcgtca
    19141 cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg gcgattctga ccggtaacgt
    19201 tggtattaac ggaggcaaca gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg
    19261 tatgccgacc ttggaaaacc cgatccagac cagcatttcg atgtttatgt ggaccgatgc
    19321 cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt gtacgcggga aagataagct
    19381 ggatgtgccg atcaaaatga tctggaacta tgccggtaac tgcctgatta accagcattc
    19441 tgaaatcaac cgtacccatg aaatccttca ggatgataag aagtgcgagc tgattgtggt
    19501 tatcgactgc cacatgacct catcggcgaa atatgctgac atcctgctgc ctgactgcac
    19561 cgcttccgaa cagatggact ttgcgctgga tgcatcctgc gggaatatgt cttacgtgat
    19621 tttcaacgat caggtgatta aaccgcgctt tgaatgtaag accatctatg aaatgaccag
    19681 cgaactggca aaacgtcttg gcgttgagca acagtttact gaaggccgta cacaggaaga
    19741 gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt cctgaactgc caacgtttga
    19801 agagttccgc aagcagggga tctttaaaaa gcgcgaccca caagggcacc acgttgctta
    19861 taaagctttc cgtgaagatc ctcaggcaaa tccactgacc acgccatcgg gcaaaattga
    19921 gatttattcg caggcgctgg ctgacattgc cgctacctgg gaattgccag aaggcgatgt
    19981 gatcgatcca ctgccgatct acacgccagg ctttgaaagt tatcaggatc cgttgaacaa
    20041 acagtatccg ctgcagctta cgggtttcca ctataaatct cgcgttcact caacttacgg
    20101 cgacgttgat gtgctgaaag cagcttgccg tcaggaaatg tggatcaacc cgcttgatgc
    20161 gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc tttaacgatc gtggtgaggt
    20221 tcatattgag gcgaaagtga cgccacgaat gatgccgggt gtggtcgcac tgggtgaagg
    20281 tgcctggtat gacccggata caaaacgtgt cgataagggc ggttgtatca acgtactgac
    20341 cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca catacaaacc ttgttcaggt
    20401 tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg attttttatt gattccagcc
    20461 gttgcaccgg ttgcaaaacc tgcgagctgg cctgtaaaga ctacaaagat ttgacgccag
    20521 aagtcagctt ccgccgcatt tatgaatatg ctggcggcga ctggcaggaa gataacggtg
    20581 tctggcacca gaacgtgttt gcctactatc tgtcgatttc atgtaaccac tgcgaagatc
    20641 cggcttgtac taaagtctgc ccgagcggtg cgatgcataa acgtgaagat ggttttgttg
    20701 tggtcgatga agatgtgtgc attggctgcc gctactgcca tatggcttgc ccgtatggcg
    20761 caccgcaata taacgaaacg aaaggccata tgaccaaatg cgatggttgt tatgaccgtg
    20821 ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc gctgcgggcg ctggatttcg
    20881 gccctatcga cgagctgcgt aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc
    20941 gagctcactt taccaaaccg aatattgtga tcaaacccaa tgccaatagc cgcccgaccg
    21001 gggataccac tggctatctg gcaaacccga aggag
//
LOCUS       DAJTNA010000060        20961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTNA010000060 DAJTNA010000000
VERSION     DAJTNA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20961
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.061"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(56..220)
                     /locus_tag="OO161_001869"
                     /pseudo
     CDS             complement(56..220)
                     /locus_tag="OO161_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280578.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            complement(299..393)
                     /locus_tag="OO161_001870"
     tRNA            complement(299..393)
                     /locus_tag="OO161_001870"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(357..359),aa:Sec,seq:tca)
     gene            686..2068
                     /locus_tag="OO161_001871"
     CDS             686..2068
                     /locus_tag="OO161_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR5462957.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2078..4369
                     /gene="yicI"
                     /locus_tag="OO161_001872"
     CDS             2078..4369
                     /gene="yicI"
                     /locus_tag="OO161_001872"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5462958.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGQDTNNQRNYMFERLDLGVGETVYGLGERF
                     TALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQSVSFEVGSEKVSK
                     VQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEAT
                     VNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKIC
                     VWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWYADK
                     LKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAHIYNELVWNVLKDTVGEE
                     EAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFWSHYIGGFEN
                     TAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYR
                     EAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAGDVQFYLPEG
                     RWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAWHEGTAFHLF
                     NLQDGHEAVCEVPDADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRNVVKVNGLQD
                     GSQAESEQGLVVKPQGNALTITL"
     gene            complement(4422..6131)
                     /locus_tag="OO161_001873"
     CDS             complement(4422..6131)
                     /locus_tag="OO161_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5462959.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSALRVNGSVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(6252..7643)
                     /gene="xanP"
                     /locus_tag="OO161_001874"
     CDS             complement(6252..7643)
                     /gene="xanP"
                     /locus_tag="OO161_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5462960.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGAALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7923..9128
                     /gene="gltS"
                     /locus_tag="OO161_001875"
     CDS             7923..9128
                     /gene="gltS"
                     /locus_tag="OO161_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5462961.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9162..11243)
                     /gene="recG"
                     /locus_tag="OO161_001876"
     CDS             complement(9162..11243)
                     /gene="recG"
                     /locus_tag="OO161_001876"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5462962.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLDIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11249..11938)
                     /gene="trmH"
                     /locus_tag="OO161_001877"
     CDS             complement(11249..11938)
                     /gene="trmH"
                     /locus_tag="OO161_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5462963.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11945..14053)
                     /gene="spoT"
                     /locus_tag="OO161_001878"
     CDS             complement(11945..14053)
                     /gene="spoT"
                     /locus_tag="OO161_001878"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5462964.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14072..14347)
                     /gene="rpoZ"
                     /locus_tag="OO161_001879"
     CDS             complement(14072..14347)
                     /gene="rpoZ"
                     /locus_tag="OO161_001879"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5462965.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14402..15025)
                     /gene="gmk"
                     /locus_tag="OO161_001880"
     CDS             complement(14402..15025)
                     /gene="gmk"
                     /locus_tag="OO161_001880"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5462966.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIKALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15283..16965
                     /gene="ligB"
                     /locus_tag="OO161_001881"
                     /pseudo
     CDS             15283..16965
                     /gene="ligB"
                     /locus_tag="OO161_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
     gene            complement(16962..17579)
                     /locus_tag="OO161_001882"
     CDS             complement(16962..17579)
                     /locus_tag="OO161_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5462967.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(17871..18695)
                     /gene="dinD"
                     /locus_tag="OO161_001883"
     CDS             complement(17871..18695)
                     /gene="dinD"
                     /locus_tag="OO161_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5462968.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVDTTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(19275..19787)
                     /locus_tag="OO161_001884"
     CDS             complement(19275..19787)
                     /locus_tag="OO161_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCR5462969.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVDSSW"
     gene            complement(20325..20875)
                     /locus_tag="OO161_001885"
                     /pseudo
     CDS             complement(20325..20875)
                     /locus_tag="OO161_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4798 a         5302 c         5607 g         5254 t
ORIGIN      
        1 agtcgtataa aaaagggtgt tatggagcgt gtaaaacaac atcttcacct taatggcgat
       61 acagttggct gagagccagc agggggcaaa tcacctgcaa ataaacacgg cacatgctgg
      121 cgcgcatatc gcggatgcgt tcgaggacag aagaaccgac aggcggattc ttcagccgct
      181 catcgtccat cacaaaacct tagaccaggt attcctgaag ccgattgaat attgagcagt
      241 agaaaatatc tggattgaca gtattacaag gggttggaga acgtcagaag aggcattttg
      301 gcggaagatc acaggagtcg aacctgtccg ggaccgctgg cggccccaac tggatttgaa
      361 gtccagccgc ctcaccggag acgacgatct tccgcgcctc gattgctaca tggaggcggg
      421 gcgcattata gctacttcct tgagtttcta catcccccag atcagttttt tccgcctaat
      481 acacgcagcg tattatcacg aaagcattat agttcatata aaacccatat tttacaatga
      541 tatatgagcg gaattaacgc taccacgatc acataaaaca agaatcataa attaataacc
      601 agatatcgga atattcgctc tcacagggat ggtttacaaa atgcgttatc ggtctcagca
      661 cccctatagc atcaaggaaa agcagatgaa gagtgaagtg ttgtccgtta aagagaaaat
      721 tggttatggc atgggagacg ccgccagcca cattattttc gataacgtaa tgttatatat
      781 gatgttcttt tataccgata tttttggcat acctgccggt tttgtcggaa ccatgttttt
      841 ggtcgctcgt gcactggatg cgatttccga tccttgcatg gggttgttgg ccgatcgaac
      901 gcgctctcgc tggggtaaat ttcgtccgtg ggtactgttt ggcgcactgc cattcgggat
      961 cgtctgtgta ctggcctata gcacgccaga tctcagtatg aacggcaaaa tgatctatgc
     1021 agcaattact tacaccctac ttaccttact ttataccgtc gtcaatatcc cttactgcgc
     1081 attgggtggt gtaatcacca atgacccgac tcagcgtatc tcgctgcaat cctggcgttt
     1141 tgtgctggcg accgcgggag gcatgctttc tactgttctg atgatgccac tggttaattt
     1201 aattggcggt gataataaac cactcggttt ccagggcggt atcgcggtcc tttccgtggt
     1261 ggcattcatg atgctggcat tttgtttctt caccactaaa gaacgcgttg aagcaccacc
     1321 tacaacaacg tctatgcggg aagatttacg tgatatctgg caaaacgacc agtggcggat
     1381 tgtcggttta ctaaccattt tcaatatcct ggcggtgtgc gtactcggtg gggcgatgat
     1441 gtattacgtc acatggattt tgggcacgcc ggaagtgttt gtcgcttttc tcaccactta
     1501 ttgcgtgggt aacctgattg gttccgcact ggcaaaacct ctgaccgact ggaaatgtaa
     1561 agtcactatc ttctggtgga cgaatgccct gctggcagtg attagcctcg cgatgttctt
     1621 tgttcccatg caggccagca tcactatgtt tgtcttcatc ttcgtgattg gtgtgttgca
     1681 tcaactggtg acacctatcc agtgggtaat gatgtccgat accgtcgact acggcgagtg
     1741 gtgcaatggt aaacgcctga ccgggatcag ttttgctggc acgctgtttg tgctcaaact
     1801 ggggttggcc ttcggcggcg ctcttatcgg ctggatgctg gcttatggcg gatatgatgc
     1861 ggcagaaaaa gcgcagaaca gcgccacgat tagcatcatt attgcgctat tcacgattgt
     1921 tccggcgatc tgttatttgc tgagcgcgat tatcgctaaa cgctactact cactcacgac
     1981 gcacaatctg aaaaccgtta tggaacagct ggctcagggt aaacgccgtt gccagcaaca
     2041 attcacctct caagaagtgc agaactaagg aacggcaatg aaaattagcg atggaaactg
     2101 gttgattcaa cctggcctca atttgattca cccgcttcag gtgttcgagg ttgaacagca
     2161 ggataatgaa atggtggtct atgctgcccc ccgtgatgtg cgtgaacgta cctggcagct
     2221 tgatacgcct ttatttacgc tgcgcttttt ctccccacag gaaggtattg tcggtgtgcg
     2281 gattgagcat tttcagggag cgctgaataa cggtcctcat tatccgctca atattttgca
     2341 ggacgtgaag gtcacaatcg aaaacacaga acgttatgct gagtttaaaa gtggcaactt
     2401 aagcgcgcgt gtcagcaaag gtgagttctg gtcactcgat tttctgcgca acggtgaacg
     2461 tattaccggt caggacacga ataatcaacg aaattatatg tttgagcggc ttgatcttgg
     2521 cgttggcgaa acagtttatg gtctgggaga gcgttttact gccctggtgc gcaatggtca
     2581 gacggtagag acctggaacc gggacggcgg cacaagtact gaacaggcgt ataaaaatat
     2641 cccgttctat atgactaacc gtggctatgg ggtgctggtc aatcaccccc agagtgtctc
     2701 ttttgaagtg ggatcggaga aagtctccaa agtgcagttc agcgttgaga gcgaatatct
     2761 cgaatacttt gttatcgacg gtccgacgcc gaaagcggta cttgatcgtt atacccgttt
     2821 tactggtcgt ccggcgctgc cgcccgcatg gtccttcggc ctgtggctaa ccacttcatt
     2881 taccaccaac tacgacgaag cgacggtaaa cagctttatc gatggtatgg cggaacgcaa
     2941 tctgccgctg catgttttcc actttgactg tttctggatg aaagccttcc agtggtgcga
     3001 ttttgagtgg gacccgctga ctttcccgga cccggaaggg atgatccgcc gcctgaaagc
     3061 gaaagggctg aaaatctgcg tctggattaa cccctatatc ggtcaaaaat cccccgtctt
     3121 taaagagtta caagagaaag gctatttact caaacgcccg gacggttcgc tatggcagtg
     3181 ggataaatgg cagccaggtc tggcgattta tgactttacc aatccggatg cctgcaaatg
     3241 gtacgccgac aaactgaaag gtctggtcgc gatgggcgtt gattgcttta agaccgactt
     3301 tggcgaacgt atcccaactg atgttcagtg gtttgacggt tccgatccgc agaaaatgca
     3361 taaccattat gcgcacatct acaacgaact ggtgtggaac gtgctcaagg acaccgttgg
     3421 tgaggaagaa gctgtcttgt ttgcccgctc ggcctccgtc ggtgcgcaga aattcccggt
     3481 acactggggt ggcgattgtt acgctaacta cgaatcaatg gcggaaagcc tgcgcggtgg
     3541 tttgtctatt ggcctttcag gttttggctt ctggagccac tatatcggcg gctttgaaaa
     3601 taccgctccg gcgcacgttt acaaatgctg gtgcgcgttt ggtttgctct ccagccatag
     3661 ccgtttacac ggtagcaaat cttatcgtgt gccgtgggcc tacgatgatg agtcctgtga
     3721 tgtggtgcgc ttcttcacgc aactgaaatg ccgcatgatg ccgtatctgt atcgtgaagc
     3781 tgcgcgtgca aacgcgcggg gtacgccgat gatgcgggcc atgatgatgg agttcccgga
     3841 cgatccggct tgtgattacc ttgaccgtca atacatgtta ggcgacaacg tgatggttgc
     3901 gccggtgttc actgaagcgg gcgatgtgca gttctacctg ccggaaggtc gctggacaca
     3961 cctgtggcac aacgatgagc tcgacggtcg ttgctggcat aaacagcagc acagcttcct
     4021 gagtctgccc atttatgtgc gtgataacac cctactggcg ctgggcaaca acgatcaacg
     4081 tcccgattac gcgtggcacg aaggcacggc attccacctc tttaatctgc aagacgggca
     4141 tgaagccgtc tgtgaagtgc ccgatgctga cggatcggtg atctttactt taaaagcagc
     4201 acgtactggc aacacgatta ctgtgactgg tgcgggcgag gcgaagaact ggacactgtg
     4261 cctgcgcaat gttgtgaaag taaatggtct gcaagacggt tcgcaggctg aaagtgagca
     4321 ggggctggtg gtgaagcctc aagggaatgc gctgacaatt acgttgtaat ctattgatgg
     4381 aatgccgggt acgtctgagt cgtacccggc gagattgatg tttactgcac cagctctgcc
     4441 gacgtaactt caccgctgga aacgatgcca gcattcatgg tttgagtcac ttgctgcttt
     4501 gcggcgttca cggcatgcaa ttgcccgcta accgtaggtt tcagcggggc attggcctga
     4561 atatctccac tggcggtaag ctgaatattg ccgtcgccag tcaacggtaa ttcaggccat
     4621 ccccattgtt gcaaaatatt caccggcaca ccgcgtccat tcaggctgat atgggtctga
     4681 cgttgtggcg tttgtgaaac actggcagtg gcttccagaa tgcctttttc agtaaatgca
     4741 ctcagttcgc tgatattcac cgtgctgctg ttggcggtca gcgccagcga cgggcgacga
     4801 acatcaacac gattgaatgt cgcggcggcg gcattcagat tcgccgagcc actccagacg
     4861 ccccatttat gatcggtaac cagcgtcagg ttggcaccgt aaccatcgag cgtggtgagc
     4921 tgccacggga agtcagggtc gatatcaatg atcagattgc ggctggcgct aaatctcttc
     4981 agttgcaggc tgtttaacca accgggtgtc gtttccatcc acaactgttg ccagtttttc
     5041 ggcaaggtat attccagccc ggcaattgcc gcatcatcaa ggatcaacgt tttcccgtca
     5101 cgcagccagt tccctgacgt tctgaccatc cccccttccc agcggctggt gaactggcgc
     5161 agcgctacgc cctgcgggga aaattccgcg tttataatcg ggtcaaataa atgcagcgaa
     5221 ccataaatga actcgctagc gttcatcgac agtttgcctt cttgtgtctg ccagtcatct
     5281 ttactgaagg tcatgttgcg caagctgaga tcgaggtcgg tcaccgccca gtccggacct
     5341 tgcaaacgag catcgatcac ttccaggcga ccaatctgca acgacgggac agagcgtaat
     5401 ggcgcaaaga agtcggttag cgatttttcg ctttgtagac gtatatccgc catgcgcagg
     5461 ttttccactt gccagctgcc gtcggcgtta cgctgcgcgt ttccggttaa tgtcccgcgg
     5521 gcgatgtcgg cacccaggtt agtcagcgta acgcgatcgt tatcaatact gccttcaatc
     5581 agtacattgg tggcaggaac atcattgagc gaaagcgatc cggcactaaa ctgaatctgc
     5641 gccttcgtac ccagcacttt accggctttt ggtgaccacg gaactacgct gccatttacc
     5701 cgcagcgcgc tcagtttcca ttcgctattc gggctattaa acgccatatc acgcagttgc
     5761 agacgatcgg ctttgaacgg tagcggcgcg gtctggtcgg tgagattcag cgtcccgttt
     5821 tccagcagga tggtatcgac atggcgtggt tcggtcagtt gccgactgct tagcgcaatg
     5881 tcgacacttt tggccaccag ggtcgcgggc tgaccatcac gaccaaacgt gacattctcc
     5941 agcacgatat gagatggcgc agaaaaacgg tgatccatcg ccccgaaggc cagatgatag
     6001 tcgctattct cggaaaccca tgcgctgata tgttctgctc cccagcgggt ttgcagaaga
     6061 aaatagaggc cagcgatcac cactaacaga gcgatgagaa tgtagagaag cagcttccca
     6121 ataaatttca tggtcttcca tcccgtgaaa tgcacataag agagttatgc acgatttacg
     6181 cgcaatcctc aaggcgggaa tggtgaaaga ggtgagactg cggcggtaat tatcttaccg
     6241 ccgccagtga attactgttt ttctggtggg aaaatcagat tcagcacgat ggcagtaata
     6301 ccgcccgcgg cgatcccgga ggagagcagg tttttcagcc attcaggggc aaactgcaaa
     6361 atcagcggct gctgagacac gcccagacca accgccagcg acagcgcgat aatcagaatc
     6421 gcccgacggt tcagcggctc acgagaaacg atacgcacac cggaggcggc gatggtgcca
     6481 aacattacaa gcgttgcgcc gcccagaacc ggttctggaa tgtgttgtac aaaaccgctc
     6541 actgccggga acagacccag cacgatcaac atcagcgcga cgacaaaacc gacatagcgg
     6601 ctggcaacac cagtcaactg gatcactccg ttgttttgcc cgaagcagga gttcgggaag
     6661 gtgttaaaca ccgccgaaac aaacgagttc aggccgtttg ccagcacgcc gcctttcagg
     6721 cgtttcatgt acagcggacc ggacactggc tgttcggaaa cgtcagaggt cgacgtgata
     6781 tcgccaatgg tttccagcga agtgatcata aagaccagca tcagcggcag cagcagactc
     6841 cattcaatgc caagaccgta atagagcggc gttggcacca taatcagttc ttgcgtcatc
     6901 ggttcgttgc tttctggcaa catgcccata aaccacgcca gcgcatatcc ggccgccatc
     6961 gcaatcacca gtgaggccac gcgtaagtaa gggttacgtt gacggttaag caggataatt
     7021 aaggctaaga ccacgcctgc cagcagcaga tttttcggtg cgccgaaggt gttatcgctc
     7081 atggctgcgt aaccgccgcc aatggacgtt aacccaacct gaattagcga caggccgata
     7141 atcatcacca caacgccaga aaccagcggc gtaataatgc ggcgcgccag atgcagaacg
     7201 cgggagatca ccatctcggt gcaacttgcc agcatcaacg tgccgaacaa agccgccatc
     7261 atggtaggaa catcagcacc accggttttc agcgcggcac cgcccataat cagcggggca
     7321 acaaagttga agctggtgcc ctgaatagac aacagcccgg agccaaccgg accccaggcc
     7381 ttaatttgaa taatcgatgc cacaccggag gcaaacagcg acatactaat aatgtgttgc
     7441 gtgtcttgtg ccggtaaacc cagcgcctgg cagattaata gcgctggcgt gatcaccgca
     7501 acgaacatcg ccagcagatg ctgacaggcg gcaaacaggg tttgaggaag cggcggacga
     7561 tcttcaagac ggtaaatcag ttcgctgttt tgagtctgcg caaccggttg cgcattttct
     7621 gactcgaggg tggaaacaga catcgaaaac aatcccgtgg tagcaaagcg ggcattttag
     7681 ctgaccgaaa gagaaaagca aacgtttgcc acatcctcat ctaaatattt aaatgagata
     7741 aatgcacttt tttatataac ttttggtatt tttctgctta aaatccatgc catttgttca
     7801 ttaacagctg agaatttgac ctaggaaagc ataactcgat acccgtcata gttcatgaag
     7861 ttgccactgc gcgatgaagt atgacgagta tgaaagagtg atgcggatac aaaggagtaa
     7921 ctatgtttca tctcgatact ttagcaacgc ttgttgccgc aacgctgacg ttgctgctcg
     7981 ggcgtaagtt ggtccattcc gtctcctttt tgaagaaata caccataccg gaacctgttg
     8041 cgggtggttt gttggtggcg ctggcgctac tagtactgaa aaaaagcatg ggctgggaag
     8101 tcaactttga tatgtccctg cgcgatccgt taatgctggc tttcttcgcc accattggcc
     8161 tgaacgccaa cattgccagt ttgcgtgccg gtgggcgtgt ggttggcatc ttcttgattg
     8221 tggttgttgg tctgttggtg atgcaaaatg ccattggcat tggtatggct agcctgttag
     8281 ggcttgatcc gctgatgggg ctgttggccg gttctattac gctttccggc ggtcacggta
     8341 cgggcgctgc gtggagtaaa ttgttcattg aacgttatgg cttcaccaat gcgacggaag
     8401 tggcgatggc ctgtgcaacg ttcggtctgg tgctgggcgg cttgattggc ggtccggtgg
     8461 cgcgctatct ggtgaaacac tccaccacgc cgaacggtat tccggatgac caggaagtcc
     8521 cgacggcgtt tgaaaagccg gatgtgggac gcatgatcac ctcgttggtg ctgattgaaa
     8581 ctatcgcgct gattgctatc tgcctgacgg tggggaaaat tgttgcgcaa cttttggctg
     8641 gcactgcttt tgaactgccg accttcgtct gtgtactgtt tgttggcgtg attctgagca
     8701 acggtctgtc aataatgggc ttttaccgcg tctttgagcg tgcggtatcc gtgctgggta
     8761 acgtaagctt gtcgttgttc ctggcgatgg cgttgatggg gctgaaactg tgggagctgg
     8821 cttcgctggc gctgccgatg ctggcgattc tggtggtaca gaccatcttc atggcgttgt
     8881 atgccatctt cgttacctgg cgcatgatgg gcaaaaacta cgatgcggca gtgctggctg
     8941 cgggtcactg tggttttggc ctcggtgcaa cgccaacggc aatcgccaac atgcaggcga
     9001 tcactgaacg ctttggcccg tcgcacatgg cgtttttggt ggtgccgatg gtcggtgcgt
     9061 tctttatcga tatcgtcaat gcgctggtaa ttaagttgta tttgatgttg ccgatttttg
     9121 ccggttaacc gatgaagcgg cggtcttctc accgccgcct tttacgcatt cgagtaacgt
     9181 tctgtctccg gcatccagcg ttctatcaag gcttttgcct gttgtgggta acgttcgtga
     9241 atatgtcgcg ccaggcgctg aacttccggg atcatcgcct gatcgcgcag taaatccgcc
     9301 actttaaatt cagcattacc cgtctgacgc gtgcctaaca attcgccagg gccgcgaata
     9361 tccagatctt tttgcgcaat cacaaaaccg tcgttactgt cgcgcagcac ttgcaggcga
     9421 atttgcgccg ttttagaaag cggcgttttg tagagcagca cgcagtgaga agccaccgcg
     9481 ccacgaccta cgcgcccgcg cagctggtgt aactgcgcca gacccagacg ctcagggttt
     9541 tcgataatca tcaggctggc gttaggcaca tcaacaccga cttcaataac ggtagtggca
     9601 accagcagat gtagctcacc ttgtttaaac gacgccatca ccgcctgttt ctcggctggt
     9661 ttcatccgcc cgtgtaccag gccaacattc aattctggta gcgccagttt caactcttcc
     9721 caggtagctt ccgccgcctg cgcttccagt aactctgact cttcaatcaa cgtacaaacc
     9781 cagtacgcct gacgaccttc agttatgcag gcatggcgca cgcggtcaat gatgtcggta
     9841 cggcgggtat caggaatagc gaccgtagtc actggcgtgc ggcctggcgg cagctcatct
     9901 atcaccgagg tatcgagatc ggcatacgcc gtcattgcca gcgtgcgggg gatcggcgtg
     9961 gcggtcatga tcaactgatg cggatggaag ccttgctgct ggcctttctc ccataacgcc
    10021 agacgctgat gcacgccaaa gcgatgttgt tcgtcgataa tcaccagcgc cagaccgtta
    10081 aactgcacct gttcctggaa gatggcgtgc gtaccaacaa tcatctgcac ctgaccgctg
    10141 gcgatggctt cctgctgaga cagtcgcgct ttgcctttct gcttaccggc gagccagccc
    10201 acttcgatac cgagcggttc aaaccagttg cggaagttat tggcgtgctg ttcggcgagt
    10261 aattcggttg gtgccatcaa tgccacctgt ttgccgtggg caatcgcacg cagcgcggcg
    10321 agggcggcga ccagcgtttt accggaacct acatcgccct gtaccaggcg catcatcggc
    10381 acatccagcg ccatatcgcg ctcgatctcc gccactacgc gtgcctgtgc gcccgttggc
    10441 ttgaacggta aggctgcgag gagtttattt ttcagcgcat cattggcgct cagcggctgg
    10501 gcatgaaaac gctgtgctcc ggcacgtaag gctaacatgc tgaggttgtg cgccagcagt
    10561 tcttccagaa tcagacgacg ttgcgccgga tgctgcccgg tttccagatc gctaagctgt
    10621 agcgtcggtg gcgggcggtg caaagtgcgc aacgcttccg gtagcgtcat cattccttgt
    10681 gacagttccg gcggcaggag ttcttcaatg gcgcaggtgt cgagcagatc cagcgcctgg
    10741 tcggttaatt tacgcagcgt ggcctgcttt acgccttccg ttgttggata aaccggtgtg
    10801 agcgtttcct gtaattctgg cgtgctgaga tcgccctgca cgcggtattc cgggtggatc
    10861 atctccgcac catatttacc gcgctttgct tcgccataag ccagtacacg gcggcccgtc
    10921 gccaggctat ttttcattgc cgcgctgaaa ttgaaaaagc gcatggtgag gatgccggaa
    10981 ccgtcgctga tctggcaggt catcatccgc cgaccgccga aggagatatt gcagttcagc
    11041 acttcgcctt ccaccgtggc ataaacgccc ggcagtagtt ctccgatggg gtagagatgg
    11101 gtgcgatctt cgtagcgcag gggaaggtgt aagagtagat cctgcacggt atgcaggttg
    11161 attttcgcca gtttgttact aagtgctgcg ccaacgcccg ttagggaact gagtgggaca
    11221 gcatctaaca ggcgacctgt catggcactt accctgcagc ctgcatagta gcccaccagt
    11281 cggcatcagc ttcgatctcg ccttgctgat tgacgtgggg ataaggcagg cctttgcgtt
    11341 ttgcgacttt cgccagcacc ggatagccgc cttcaaacaa caggcgttgt tgctctgctt
    11401 ccggcaacat gctgttttca cgcaggtaca tgcctgcatt ttgccgctga cgctgggctt
    11461 cgtaaagaat gagggctgag gcaacggaaa cattcagcga ctgcaccatg ccgatcatcg
    11521 gaatgatgat gtcctgatcc gccagggcca atgcttcctg cgtgatgccc gttttctcct
    11581 gtcccatcaa aatgcaggtc gggcgagtgt aatcaatttc gcggaaatcg acagcgttat
    11641 cagaaagatg ggttgccaga atctgcatgc cctggccttt gagatgagcg acggcatcgc
    11701 caatggtgcg gtgtgttttc acctgtaccc agctgttact acccgccgct gccgaagcca
    11761 tggtgcgcat gcggctacca ggccagacgg cgtgaacttc atgtacgcca acggcatctg
    11821 cggtacgaat aatcgcagaa acgttatgag gtttgtggac ctgctccatg cagacggtca
    11881 gatcaggctg ccgcctggcg agcatttcgc agatgcgtgc ataacgtgtt gggttcataa
    11941 aacattaatt tcggtttcgg gtgactttaa tcacgtctgg catcacgcgg attttgcgca
    12001 tgatattcgc cagatgcaca cggtcacgag cggtcagacg aataaaggcg ctgtagacgc
    12061 gaccatcttt ctcttccgta ttcaaacttt gaatattcga agtcgtggtg ttaattgccg
    12121 ccgtcaggtt tgccagcgca ccctgatgat tgaacatctc caccttgatt tcggtgatga
    12181 actcctgcgc cgtctcttta tcccattcca cagccataaa cttctctggc tctttctggt
    12241 agccacggat gttacggcag gattcatggt ggatcaccag acctttaccg gggctgacgt
    12301 gggcgataat cgggtcgcca ggaatagggc ggcagcattt cgcaaaggtg atcagcacgc
    12361 catcagcgcc tttgatcggc agatgcccgt ggctttgagt cgccggtgga atggaggcgt
    12421 ccccatgttg tagatttttc gcgaccacca cgctcattgc gttaccaagg ccgatttctg
    12481 ccagcagatc gtcaagcgtt gccagcttca tgcgatccag ctcgcgctga atattttcct
    12541 gcgggatttc attgagctta cggctaccac ccaaagcatg gttgagcaga cgacggccca
    12601 ggcttacaga atcatcacgc ttgaggtttt tcagcaactg acgaattttg gcgcgcgctt
    12661 tcgagctaac gacaaagttc agccaagcgg cattcgggcg agcgcccgga gcggtaatga
    12721 tttcaacggt ctgaccgcta gtaagcggct gcgacagcgg gtaaggctgg cggtcgacgc
    12781 gtgcgcccac gcaggcatgg ccgatatcgg tatgcactgc ataagcgaag tcgacgggcg
    12841 ttgcaccggc aggcagctcg acaatgcgcc cttccggtgt gaaaacgtaa atctcatccg
    12901 ggaagagatc ggatttaacg ctctcgataa attcaaacga actaccggcg ctctgttgca
    12961 gctccagcag gctttgcatc cagcgctggg cgcgtatttg tgcggtagta ctggtttcgc
    13021 cgtgctcttt ataagcccag tgcgcggcaa cacccatctc cgccatctga tccatatctt
    13081 cggtacggat ctggacctca accggcacgc cgtgcgggcc aatcatcgag gtgtgcaacg
    13141 actgatagcc gttcgctttt ggaatggcga tatagtcttt cacgcggccc ggacgcggct
    13201 tgtacaggct gtgcatctgg cccagcacgc gataacaggt gtcagaatca ttgacgatca
    13261 cgcggaaagc gtagatgtcc atgattgagt gaaaacgctg ctctttgagc accattttgc
    13321 agtaaatcga ataaagatgc ttttcgcgac cactgacgcg gcacggtatt cccgcttcct
    13381 gcaaacgccc ttcgatttca gaaagaatct tctggatcat ctctttacgg ttgccgcgcg
    13441 cggctttcac cacttctttg attacgcgat aacggttggg atacagcgcc tcaaaaccca
    13501 gctcttcgag ttcggtttta atgtggtgga tacctaaacg gtgcgccagc gggctgtaaa
    13561 tttcgagagt ttcacgggcg atgcggcgac gtttgtccgg gcgaagtgag cccagcgtgc
    13621 gcatgttgtg ggtacggtcg gcaagtttga tgaggatgac gcggatatcc tgcaccatcg
    13681 ccataatcat cttgcgaaag ttttcggcct gcgcctcttt cttatcgcgg aacttgagtt
    13741 tatcaagttt cgacaccccc tctaccagct cggcgacgct tttaccaaaa agctgttcca
    13801 tatcctggta ggtggcggga gtatcttcaa tcacgtcatg cagcagcgcc gccatcagcg
    13861 tttcatagtc gagtttcatc tcggccagaa tgcaggcaac cgctaccggg tgcgtgatat
    13921 agggttcacc gcttgaacgt gtttgcccct cgtgagcatc acgtgcaacg agatacgcct
    13981 gccgcagacg cttgatttgg tcttccggca ggtaggtttg aatcagttga ttcaggcttt
    14041 caaacagata caagggcgac ccgctttgtg attaacgacg accttcagca atagcggtaa
    14101 cggcttgtaa ttcagcggct tcctgctctt gctgttcctg gcgttcgcga acgtcgagga
    14161 tctggttgtt gatcagacct tcttcgattt cgcgcagcgc gattacagtg gttttatcgt
    14221 tttcttccgg taccagcgga tcctttccgc ctacctgcat ctgacgagcg cgacgcgcgg
    14281 cgaccagtac caggtcaaaa cggttaccaa ttttctctac agcgtcctga acagttacgc
    14341 gtgccatact taaaaagctc cacaggtgaa gaaatgactg ggcatgatac tgaaatcagg
    14401 ttcagtctgc caacaatttg ctgattaaag cgtcatgacg ctgcttttgg cggctcatgc
    14461 gcagacgttc ggcgcgaata atggtcttca aatcggtcaa cgcggtatcg aagtcatcat
    14521 tcacaatcag gtaatcatat tcggcgtaat ggctcatttc tgcaacagct tgcgccatac
    14581 gctttgcaat gacctcttcg ctgtcctgac cgcgaccgcg tagacggcgg tccagttcaa
    14641 ttttagacgg cggtaaaata aagatactcc gcgcgtgcgg catcttctgg cgaatttgct
    14701 gcgcgccctg ccagtcgata tcgagaaaaa catcgacacc ggtcgccagt acttgctcaa
    14761 tggcctcacg cgaagtgcca tagtaattac caaaaacttc tgcgtgttcg aggaacgcat
    14821 ctctgctaat catttcttta aattcatcat gattaacaaa gaaataatgt tcaccgtgga
    14881 cttcaccagg acgcggttgg cgtgtggtgt gtgaaacaga aacctgggtg tcatacaacg
    14941 gttgggtttt taataaagcc ttaatcaggc tggatttacc cgcgccactg ggggcagaaa
    15001 caatataaag cgtgccttga gccatgagta tctttcgtat gtgattagcg aaataaagcc
    15061 tacatacgag cttattatac acggcgcggc catgtgacgt agcccttgct cacacttttt
    15121 gcgcaaagtt gttgcgtttt gcgtagctgc ttccgcattt gcctgtgatt tactgcaata
    15181 aagacgattc tcaggaagcg tcctcgcaga ctccgttttt gccactcggc tttgccttca
    15241 ccacgttgta tatctgccag cctgactaca gcggaggtga agatgaaagt atggatggcg
    15301 atattaataa gtatcttgtg ctggcaatca tctgtgtggg cggtctgtcc ggcctggtcg
    15361 ccagccagag cacaggaaga aattttccgc cagcaacagc aaataaaaca gtgggacgat
    15421 gactactgga aggaaggaaa aagtgaggtg gaagacggtg tttacgatca gttaagcgcc
    15481 cgtcttacgc agtggcaacg ctgctttgtg agcgagcccc gcgacgtgat gatgccgcct
    15541 ttaaatggtg cggttatgca tccggttgcc catacgggtg tacgcaaaat ggcggataaa
    15601 aacgcattaa gtctgtggat gcgagaacgt agcgatcttt gggtgcagcc aaaagttgat
    15661 ggcgttgcgg taaccctggt ttatcgggac gggaaactga acaaagcaat cagtcgcggt
    15721 aatggcctga aaggcgagga ctggacgcag aaagttagct taatttccgc tgtgccgcaa
    15781 accgttagcg ggcctttagc caacagtacg cttcaggggg aaatatttct ccagcgcgag
    15841 gggcatatcc aacaacaaat ggggggaata aatgcccgcg caaaagttgc tggcttgatg
    15901 atgcgccagg acgatagcga cacgctgaat tctctgggcg tttttgtctg ggcatggccg
    15961 gatggaccgc agttaatgtc tgatcgttta aaagagctgg ctaccgcagg ttttactcta
    16021 acgcagacgt atacccgtgc ggtgaaaaat gctgatgaag ttgcgcgcgt acgcaatgag
    16081 tggtggaaag cggaattacc cttcgtcacc gatggcgtag ttgtacgagc ggcgaaagag
    16141 ccagaatccc gccattggct accgggccag gcagagtggc tggtggcctg gaaatatcaa
    16201 cctgtagctc aggttgccga agtgaaggcg attcagtttg cggtgggtaa gagcggtaaa
    16261 atatcggtgg ttgcgtcact cgcatctgtc atgctggatg ataaaaaagt ccagcgggtg
    16321 aatattggtt ccgtcaggcg ctggcaggag tgggatattg cgcctggtga tcagattctc
    16381 gtcagccttg ccggtcaggg tattcctcgc attgatgatg tggtgtggcg cggtgcagaa
    16441 cgtacaaaac cgacaccgcc agaaaaccgc tttaactcgt tgacctgcta ctttgcttct
    16501 gatgtttgtc aggaacagtt catttcacgc ttagtctggc tggggtcaaa acaggttctt
    16561 gggctggatg gcattggtga ggccggttgg cgcgcgctgc atcagactca tcgctttgag
    16621 catatctttt cttggctttt attaacgcca gagcaattac agaacacgcc ggggatcgcg
    16681 aaaagtaaaa gtgcgcagtt atggcatcag tttaatctgg ctcgtaagca gccttttact
    16741 cgctgggtga tggcaatggg aataccgcta acccgggcgg cgcttaatgc cagtgatgaa
    16801 cggtcctagt cgcaactttt atttagcacg gagcagttct ggcagcagct gccggggact
    16861 ggatctgggc gcgccagaca ggttattgaa tggaaggaaa atgcgcaaat caagaagctg
    16921 ggcagttggc tggctgccca gcagatcaca ggttttgaac cttagtgacc ttcgtggctg
    16981 tagtaaaaga ccggtaatcc cagcttaaga cgtagcgcca gtaagcgggc aaaaaagccg
    17041 aataccaggg tggagataat caccacatca tggttagaaa catagtgttg cagcgcgatg
    17101 tacagcacgg cggaggcaaa tgagacacca gcatacaact ctttctggaa taccagtggg
    17161 atgcgtttac agaacatatc gcgcaaaacg ccgccaaaca cgccggtggt taccgccgcg
    17221 acaacggcaa taattgggcc gtgccccata tccagtgcaa cctgtgcgcc gatgatagaa
    17281 aagactacca gtccgagcgc atcgagcacc agaaacactt tgcgcaggta aggcattaca
    17341 ggagcaacga tagtggtgag tactgcggcg gtagcgacga taatcacata ttccgggtgt
    17401 ttgacccagc cgagcggata gtggcccagc agaatatcgc gcactgaccc tccgccaatt
    17461 gcggtggcgg tcgcaataat aattacgcca aatgtatcca tgcgccgtcg tccggccgcc
    17521 agcgcccccg tcatggcttc ggcagtgatg ccaaccaaat acaaaatgtg taacagcatg
    17581 gttctctccc taaaaatctg tctggcaggg tagcgatttg tgcgcattgt cacgattgag
    17641 attttttaag gtaagaggaa attgataact tgtagttatg tgaaatcgct attttctgta
    17701 gcgtagatgc attattctga gttcttgtct gagaaaatgc ataagaaaaa ataggttaaa
    17761 attgaggggt ggaaaagaaa ggtgcaatgt ttagccattg tgttcactgc acctggactt
    17821 tggcttacgc atcgggtatc tcaattaaga aaaagagttt ttagtttctg ctatttcttc
    17881 tctgtaattt ttacactgtt ttccaactgc ttgatgctga cctgcggggt cggcaactcc
    17941 tcaggcatgg ttccgccaag ttcctgaatg gtttgcctca ccttgcgacc cacgtcaaag
    18001 tgtgtggtgt ttgcttgctg ttttgaattc acgccatccc gcttgagttt ttcttctgtt
    18061 tgggtagctc gaaagagatt agccgccagt tctgttgaac ccatatgatc caggatattc
    18121 tgattctttt tcagcccctt ccgctgatga atagctttct gatctaatcc accatacagc
    18181 ccccggtaac catgattctg aaatatggcg aaatcgatag ctgtcgctac cccagcttgc
    18241 tgcgctgcct caaccaattg tttgttatgt tctttcaatt catttctcag aaaaagacgc
    18301 ttttcgtctt cacgaagttg cctgaaagct tcatcgtcag ccagctcctg ccgtcgggtc
    18361 tggatagcaa aataagtttg ccctgccgca ataaccggtt tcgcagggtc gccgttttgt
    18421 actaccaggt aacaggcata gcgggagaga tgaacatctt caagttctcg ttgagcccct
    18481 gaacctaata caaccatttt ggtggtatca acgaaatggt cagatgcagc ttgattgctg
    18541 gcttcgcacg cttgagtcgc gcgggccagt accttttgaa aattgcgcca gtcccgataa
    18601 tccagtaacg gtgccaattc tctagctgac caaaattctt gcccttcagt gccataatgc
    18661 cgtatttctt caaaaggttg atgatgttcg ttcattgccc actccatgtg atatcggttg
    18721 caaagaaagt aaatctgtaa ctgtatttat atacagttgt gagtatctca cgttctcagt
    18781 gtgctgggca aaaaaacatc gcaaaatctg gagcaggcac gcaaaacaga attgaagttg
    18841 cggggaagaa aaatgccgct ctgtttgagt gggcggcatt tgtgaaggct gaaaagtaag
    18901 aattggcgtt acgagttgaa tctgtttatt gagtagtggc tttaaacgcc cgtggttgct
    18961 gtgtttcttg taatgcgttt tctcagtagg tatacccttg tgtacacggt attttgaggc
    19021 gctgtctttg gctgctcgtt ttatgctctc atgcttgctg ttctggcctc ttatcttttg
    19081 cgccttattg tatgatagtg ctatcccaca ataaggaacc tgccagctat gagaatgacc
    19141 agccgcaaaa aagagatact tagctacttt gaaccggata acctcgaatg ggtgactggt
    19201 gagattggtg cgccgccgtt cgatgtgtcg ggtgtggcct acctgcttca tggcatggtg
    19261 tcgttcgata agcgtcacca gctcgaatca acccggcgct caatatccag catcaggaaa
    19321 ttgtggatct cggcctgctc aggattatca gactctctgg cctgtaatag caattcattg
    19381 taaccatgca agccgcctga gttttccacc ggggtggtgc ctttcccacg caggcagcta
    19441 atgggctgat tgctcgtatc gtgtgtttgc ttctccagca caatgacgca atcccaccag
    19501 tcgccaaagt catacgtgta aaccagcttg tcaccatcat tctggagcgc tgcccatagt
    19561 ggtgtgattg ccgcattacc catgctttca tcctcacccc atacgcgata ctcgctaccg
    19621 ggatcacctt tacgcccttt ctcgaacata tacaggtgtt cgtcattcca gccaaaagcc
    19681 aactggatca ccgcgtgaag ttcactaaag gggatttgct ctggaaccag cacacgccgc
    19741 catataggtg gtttgctgtc tcggatggtt atctttagct ggtacatgca aatcctgaat
    19801 aaatgcgaat cggaaggata acgatactga cgtactcaca attctgtaaa ggtgaataaa
    19861 ggaaatttgc ttaatacgca taagttacat gcggaataat cccgtatcta cctgcgcggc
    19921 gctggcttct tccactggta ggcaggtgct cgcatcattt cgcggaacct gtcgcgccgg
    19981 gctatatcgg cattaactga gcgttctatc tcccgttgat ctgcctgtgt aagctggtgg
    20041 cctccatgct caagtttggc gatggtcaat aaggctattt cgcgtggtgt catacgtcat
    20101 accctctgga aggatgaaac acgcccggat taaatttcat ttttcgcggg tatgaaaaaa
    20161 cgatggggcg ggcagtcctt aaggctcagg attgcagaga tttggcctcc ccctcccggt
    20221 gatagtttat tgcttgatgt ggacggcttg ctatattggc ggcgaatgca aattgtcgca
    20281 gaagtctatc ccgttctctg acgccccgtg cgggcgtttt gcttttatag agtcaggcca
    20341 atgcctctgg ctgcttttcc tgctttacgc tcaataacgc ccgtgattct gtccactacc
    20401 gggctgaatg cctgccagta agccatctcc ataactgata actctgcccg cttcctgtca
    20461 aacgtagccc agcgcatccc ctttggtttt gggaacatgc gaatgtcatt aaacagatta
    20521 ttggcaaggt cattgctgcc ccatatcgcg catcgctgct ggcgtatctt catgcgcatc
    20581 ctgtccagta tgtcggcact ctggctttta tagtgaagtt acagtggggg caagcgtacc
    20641 attcccggac gccatacccg gccttcgtgg ttgtggtgcg aatagcccgc gtcacgccgt
    20701 tgattgtcat ggcgtagctg tcggcttgcc ggaaggcata caacgttccg ctttcggtct
    20761 gggtataaag ctgcgtaccc ggttcagcat ccgccagcct gctacgcatg ttcgcaagga
    20821 atggcaaccc tatacggggc aggtcggtgg tatagctgcg cgttctgttt ctcactggct
    20881 ggcctcctgc gaattactgc gcattaactg cgaacggtgc gcaaattgaa tacgaaataa
    20941 tatgcgtatt ttactgtaaa t
//
LOCUS       DAJTNA010000061        20920 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTNA010000061 DAJTNA010000000
VERSION     DAJTNA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20920
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.062"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(250..996)
                     /gene="cutC"
                     /locus_tag="OO161_001886"
     CDS             complement(250..996)
                     /gene="cutC"
                     /locus_tag="OO161_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5462970.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANLLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1010..1576)
                     /locus_tag="OO161_001887"
     CDS             complement(1010..1576)
                     /locus_tag="OO161_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5462971.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTAEHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1792..3525
                     /gene="argS"
                     /locus_tag="OO161_001888"
     CDS             1792..3525
                     /gene="argS"
                     /locus_tag="OO161_001888"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5462972.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3702..4190
                     /locus_tag="OO161_001889"
     CDS             3702..4190
                     /locus_tag="OO161_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5462973.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4310..4702)
                     /gene="flhe"
                     /locus_tag="OO161_001890"
     CDS             complement(4310..4702)
                     /gene="flhe"
                     /locus_tag="OO161_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5462974.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4702..6780)
                     /gene="flhA"
                     /locus_tag="OO161_001891"
                     /pseudo
     CDS             complement(4702..6780)
                     /gene="flhA"
                     /locus_tag="OO161_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(6773..7876)
                     /gene="flhB"
                     /locus_tag="OO161_001892"
                     /pseudo
     CDS             complement(6773..7876)
                     /gene="flhB"
                     /locus_tag="OO161_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(7959..8603)
                     /gene="cheZ"
                     /locus_tag="OO161_001893"
     CDS             complement(7959..8603)
                     /gene="cheZ"
                     /locus_tag="OO161_001893"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5462975.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8614..9003)
                     /gene="cheY"
                     /locus_tag="OO161_001894"
     CDS             complement(8614..9003)
                     /gene="cheY"
                     /locus_tag="OO161_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5462976.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9018..10067)
                     /gene="cheB"
                     /locus_tag="OO161_001895"
     CDS             complement(9018..10067)
                     /gene="cheB"
                     /locus_tag="OO161_001895"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5462977.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTSKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10070..10930)
                     /gene="cheR"
                     /locus_tag="OO161_001896"
     CDS             complement(10070..10930)
                     /gene="cheR"
                     /locus_tag="OO161_001896"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5462978.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10949..12479)
                     /locus_tag="OO161_001897"
                     /pseudo
     CDS             complement(10949..12479)
                     /locus_tag="OO161_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(12537..13952)
                     /locus_tag="OO161_001898"
     CDS             complement(12537..13952)
                     /locus_tag="OO161_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HCR5462979.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYKNYYTALTELIDYLDYGNTGAYFAQPTQGM
                     QNIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQ
                     ASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIAD
                     SSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSVQAA
                     KEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIVQ
                     VALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPS
                     RPASEQPPAQPRLRIAEQDPN"
     gene            complement(14097..14600)
                     /gene="cheW"
                     /locus_tag="OO161_001899"
     CDS             complement(14097..14600)
                     /gene="cheW"
                     /locus_tag="OO161_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5462980.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKEQEICGYDQ
                     VTRIANTPAFIKGVTNLRGVIVLIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14621..16292)
                     /gene="cheA"
                     /locus_tag="OO161_001900"
                     /pseudo
     CDS             complement(14621..16292)
                     /gene="cheA"
                     /locus_tag="OO161_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
     gene            complement(16297..17223)
                     /gene="motB"
                     /locus_tag="OO161_001901"
     CDS             complement(16297..17223)
                     /gene="motB"
                     /locus_tag="OO161_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5462981.1"
                     /translation="MKNQTHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17220..18107)
                     /gene="motA"
                     /locus_tag="OO161_001902"
     CDS             complement(17220..18107)
                     /gene="motA"
                     /locus_tag="OO161_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5462982.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDCLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18234..18812)
                     /gene="flhC"
                     /locus_tag="OO161_001903"
                     /pseudo
     CDS             complement(18234..18812)
                     /gene="flhC"
                     /locus_tag="OO161_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
     gene            complement(18822..19160)
                     /gene="flhD"
                     /locus_tag="OO161_001904"
                     /pseudo
     CDS             complement(18822..19160)
                     /gene="flhD"
                     /locus_tag="OO161_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
     gene            19940..20368
                     /gene="uspC"
                     /locus_tag="OO161_001905"
     CDS             19940..20368
                     /gene="uspC"
                     /locus_tag="OO161_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR5462983.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(20375..>20920)
                     /locus_tag="OO161_001906"
     CDS             complement(20375..>20920)
                     /locus_tag="OO161_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCR5462984.1"
                     /translation="TQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLN
                     QHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANEL
                     TSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQ
                     IVPRSAESQQRDKVATFPKLA"
BASE COUNT         5094 a         5673 c         5022 g         5131 t
ORIGIN      
        1 attgctaagg aataaccctg ttgttattta gaatatatat ttgtcagttt tagcaattta
       61 aaccccataa ttaatgtgaa tatataaaat atttttagca caacgctatg ttcactgtca
      121 cccgctcaac agatcacggt tatcgtacgt tttttatact gctcagggat aaaaaaaggc
      181 ccctgttgaa attgcagggg cctggtacga gcaagcatca tattgggcga catgatgcaa
      241 cggtaaaaat catttggcct gatggcgttc aatgattcct ttcatttcag caaccgccgc
      301 cccgtctacg acataacgcg aatactcgtc cgcgtgtgca tctgatgaca tggacaatcc
      361 ttgattacga taacgcatcg gtgaagcttg ccacgctccc gcggagctat ggacttccag
      421 cactccggca tcgaggaagt ggtgcaggtt ttctgcacgg actcctgctc cggccataat
      481 gattggagca tcacgatggg caataagttc cataattttt gataaacctt gcagtgcatc
      541 tgatttttgc cctgatgtca gcacccgggc gatgcccaat tccgcaagat tactgagtgt
      601 atttaaaagg ttagcgcaca tatcgaaggc gcgatgaaaa gtcactgcca gcggaccggc
      661 agcagccatt attttttcca ttcgtggcat atcgacattc ccgtcaacat cgagcacgcc
      721 cgtcaccagt ccaggaaaac ctaattcgcg gactgtgcgc acatcctcaa gaatggcggc
      781 aaactcaccg tcgctgtagc aaaaatcacc accgcgtggg cggataatcg gatgcacagg
      841 gatcgtcacc cactggcgca cggatttcag tacacccaac gacggcgtta agcccccctc
      901 ttttggggct gcgcataatt caactctgtc tgcgccgttt tgctgcgccg ttagtgcaca
      961 ttccatgctg taacagcaaa tttccagtaa tgccattttt actccttaat tacgccgact
     1021 gctcgctggc aacgatctct tcaatggacc acggatgaaa cttaatagtt gttttgccat
     1081 cggtgaccgc cagcgtcgga ttcggtaatc gctcatactc accttttggc gaactggttt
     1141 tcactgagat ccccggtagg ctgagtcctt catcagaaag caatgccagc gcacgggcat
     1201 tcagcgtttc tggatcaccc ggcagaacga tttcaatatg ttcccaacct tcgtgtgggt
     1261 aacgtttttc cccgggccac ggtagctcca caatggaaaa ctgccagtgc gcaacctgta
     1321 ccggttcatg caatttaaac agacaaatcg gtctgccatt gatcatattt tctgacaaaa
     1381 gctcgccaca ctgttcaaaa ccgcgacgcc agcgctcagc ggtgacgttt tgatggcaac
     1441 gcaaagaaat gtgctcggca gtcagcggag tgatatccaa ccccagacgg cgggagagtt
     1501 catctaatgc gtgggtaaat cgcggtaaat ccgatgcaat atcctgcagc tcgtcgatag
     1561 attgccagtt cgccataatc actcttcgtc tttcagtaaa agcgttaatt taccctgttg
     1621 ccctgcgcca accaaccgct gatttcacgc cgcttctgat gcaatagtga aaacggcaat
     1681 acgccacgcg cacgttgctg acgaaaacag ccatttgcag tatactcccg ccctaatttc
     1741 tttaactggt gcgggcaatt tttgctcgct tcatcaatgt aaggtattcc ggtgaatatt
     1801 caggctcttc tctcagaaaa agtccgtcag gccatgattg cggcaggcgc gcctgcggat
     1861 tgcgaaccgc aggttcgtca gtcagcaaaa gttcagttcg gcgactatca ggctaacggc
     1921 atgatggcag ttgctaaaaa actgggtatg gcaccgcgac aattagcaga gcaggtgctg
     1981 actcatctgg atcttaacgg tatcgccagc aaagttgaga tcgccggtcc aggctttatc
     2041 aacattttcc ttgatccggc attcctggct gaacatgttc agcaggcgct ggcgtccgat
     2101 cgtctcggtg tttctacgcc agaaaaacag accattgtgg ttgactactc tgcgccaaac
     2161 gtggcgaaag agatgcatgt cggtcacctg cgctctacca ttattggtga cgcagcagtg
     2221 cgtactctgg agttcctcgg tcacaaagtg attcgcgcaa accacgtcgg cgactggggc
     2281 actcagttcg gtatgctgat tgcatggctg gaaaagcagc agcaggaaaa cgccggtgaa
     2341 atggagctgg ctgaccttga aggtttctac cgcgatgcga aaaagcatta cgacgaagat
     2401 gaagagtttg ccgagcgtgc acgtaactac gtggtaaaac tgcaaagcgg tgacgaatat
     2461 ttccgcgaga tgtggcgcaa actggtcgac atcaccatga cgcagaacca gatcacctac
     2521 gatcgtctca acgtgacgct gacccgtgat gacgtgatgg gcgaaagcct ctacaaccca
     2581 atgctgccag gaattgtggc ggatctcaaa gccaaaggtc tggcagtaga aagcgaaggg
     2641 gcgaccgtcg tattccttga tgagtttaaa aacaaggaag gcgaaccgat gggcgtgatc
     2701 attcagaaga aagatggcgg ctatctctac accaccactg atatcgcctg tgcgaaatat
     2761 cgttatgaaa cactgcatgc cgatcgcgtg ctgtattaca tcgactcccg tcagcatcaa
     2821 cacctgatgc aggcatgggc gatcgtccgt aaagcaggct atgtaccgga atccgtaccg
     2881 ctggaacacc acatgttcgg catgatgctg ggtaaagacg gcaaaccgtt caaaacccgc
     2941 gcgggtggta cagtgaaact ggccgatctg ctggatgaag ccctggaacg tgcacgccgt
     3001 ctggtggcag aaaagaaccc ggatatgcca gctgacgagc tggaaaaact ggctaacgcg
     3061 gttggtattg gtgcggtgaa atatgcggat ctctccaaaa accgcaccac ggactacatc
     3121 ttcgactggg acaacatgct ggcgtttgag ggtaataccg cgccatacat gcagtatgct
     3181 tacacgcgtg tattgtccgt gttccgtaag gcagaaatta acgaagagca actggctgca
     3241 gctccggtaa tcatccgtga agatcgtgaa gcgcaactgg cagctcgcct gctgcagttt
     3301 gaagaaaccc tcaccgtggt tgcccgtgaa ggcacgccgc atgtaatgtg tgcttacctg
     3361 tacgatctgg ctggtctgtt ctctggcttc tacgagcact gcccgatcct cagcgcagaa
     3421 aacgaagaag tgcgtaacag ccgtctgaaa ctggcacaac tgacagcgaa gacgctaaag
     3481 ctgggtctgg atacgctggg tattgagaca gtagagcgta tgtaatcgat ttttcgtgag
     3541 agtaaagcct gatcagggtt acctgatcag gcttttttat tgccatctaa atgtattctg
     3601 aaaatggaca tgccattgtt ttctgactgt tggataagag gccagaagcg taatatccgg
     3661 ccccagggaa acgataacgg ttgaatttaa ggaataccgc agtgtttaaa tttcttgtat
     3721 taacattagg cattatctct tgccaggctt acgcagaaga tacggttata gtaaacgacc
     3781 atgacatttc agccatcaaa gattgttggc aaaaaaattc agatgatgat actgacatta
     3841 acgtgatcaa atcatgcctg cgacaagaat acaatctcgt cgatgcgcaa ttaaataaag
     3901 cctatggtga agcttatcgt tatatagaac aagtgccacg cacaggtgta aaaaaacctg
     3961 ataccgaaca acttaacttg cttaaaaaat cacagcgagc ctggctggat tttagggaca
     4021 aagaatgtga attaatcctt tcaaatgagg acgttcagga tttaagtgac ccttattctg
     4081 aatcagaatg gctctcatgt atgatcatac agaccaatac gcgaactcgc cagttgcagc
     4141 tataccgtaa ctctgaagat ttttatccaa gccctttgac aagaggataa ttcacatctt
     4201 tttggcatgt tttgttgcaa gctattcctg ataaataatt gcaacaagac atcgagcctt
     4261 tttcactgag ttattaaaca tactcgcgag cgcgtaattt ttttgtcctt cagcgataat
     4321 tcacaatcac ttcattacgt tgtaccttta gcggtggaat taaccgccca ccgcctggca
     4381 cttcccagat aaatcgcaac ggttctgctg ccgctatacc ggaaaaggcc acggtggttc
     4441 cgctctgccc ctctaattcg acacaacgag attgcgaaca caagcgaacc cgcagtcctg
     4501 aaggtgtcgg gccgataagc tgataacgcc acgctaccag cgtcatcaat cctgaagcag
     4561 gttgtcgcgt agaaagaggc gcagacgaca tcgactcacc gcgatgattt agcgtaattc
     4621 ctacactact tgcctgccac atcccctccc cggcggcttg caccagcagc ggaaacaata
     4681 atattgctaa taaggttctc attatttgcc gccaattgtc gccgtcatgc ggatatgtcg
     4741 gttatcagac agttccagat tcgacaggac cactaactgc ggcaagctgc ggcgcaggaa
     4801 gcgagacaat aatggtcgca gcgcgtggtt caccaacaat actggcggcg cacccagcat
     4861 ctcctgacgg gatagcgctt cctgagtttg cgccagtaaa cgatccgcca gccctggctc
     4921 cagtcctccc ccgccctgca gcgcctgtag taacaaacgt tccagcggtg tatcgaggcc
     4981 aataacatgg acttcatctt tgccaggaaa ccactgctgg gtaatcgccc gtcccaacgc
     5041 cacgcgcacg acggcggtta attcatgtgg atcgctttgg atgggcgcat gttccgccag
     5101 cgtttcgaga atggtgcgca tatcgcgaat cggtactttt tcatcgagga gattttgcag
     5161 cactttatgc agtgtggtga gcgtgacgac gccaggaacg agatcttccg tcagctttgg
     5221 catctcctgg gcgacgcgat ccaacagctg ttgcgcctcc tgacgaccaa acagctctgc
     5281 ggcatgctgg ctaatgaggt ggttaagatg cgttgctacc accgtgctgg cctcaaccac
     5341 tgtgtacccc tgaatctgcg cctgttcttt tagcgcactt tcaatccaga tagcattcag
     5401 gccaaatgcc ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg ttccagggtt
     5461 aatcgccaac cagcgccccg gataagcatc accactgcca atctccacgc ctttcatcaa
     5521 aatgcgatag cgggcaggtt gcagatccat attgtcgcga atgtgcacca ctggcggcag
     5581 aaatcccatc tcctgggcaa atttcttgcg gatactgcgt atacggccca acaactcacc
     5641 atcctgctgg aaatcgacca tcgggatcag tcgataaccc acttccattc ccagagaatc
     5701 ttccagttgt acatcgttcc acgtcgcttc gacaacggta ttattctctg ccatttttac
     5761 cggtttgggt tcggcaggcg ctttttattc gcgtccgcgt atccaccagg ccagccccag
     5821 caatccggca gtgaacagca aaaataccag gttcggcatt ccaggcacca agccgagtaa
     5881 accgagcacg gcggcgctta acaacataac gcttgggtta ctgaaaagct gattcaccat
     5941 ctgctcgcca acatcctgat cggtgctgac acgcgtaacg atgacccccg cggcggtaga
     6001 aatcaccagc gccggaattt gtgccaccag accgtcgcca atggtcaata gcgtataact
     6061 ttccgccgcg tgtcccatgc tcatgccatg ttgcagcacg ccgaccagca acccgccgac
     6121 aacgttaatg accatgatga ggatcccggc gatggcatcg ccgcgaacaa acttacttgc
     6181 cccgtccatt gagccgtaaa aatcggcttc ctgagtcact tcggagcggc gttttttcgc
     6241 ctcatcttca ccaatcaatc cagcgttaag gtcggcgtca atcgccatct gcttacccgg
     6301 cataccatcg agaacaaagc gcgcacccac ttctgcgata cgcccggcac ctttggtaat
     6361 gaccataaag ttgatgatca cgagaatgac aaacaccacg atacctatag cgaaattgcc
     6421 accaacgagg aagtgaccga acgcttcgac caccttccct gccgccgccg cgccggtatg
     6481 cccttccatc aaaatgatac gggttgaagc cacgttaagt gccagacgca acaacgtagt
     6541 aaacaacaga atggtcggaa acgcagcaaa ctcaagcgtg cgctgggtaa acatcgccac
     6601 cagcaacacc atgatcgaca aggcaatatt gaaggtaaac aacaggtcga gtatgaatgc
     6661 gggcagtggc agcaccatca tcgacaagat caacaggatc aaaatcggtc cggcaaggat
     6721 ctgccattgt gtcgatttca ggtttgcggg caggcgcagc atcgcggcca gattactcat
     6781 gggtcggttt ctcgttaata aaatccaggg cttccggcac cggaagatga gtaggttgta
     6841 cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagatccag gccagcactt
     6901 ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca tgtcgataca
     6961 gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg ccaatttcac
     7021 gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg ctcattttgt
     7081 tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg acatccgctt
     7141 tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga cggatccgtc
     7201 ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc tgccgcgaca
     7261 ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc accattggaa
     7321 tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca ttacccatgg
     7381 cggtaatcgg agactcggcc agtaagcgca tcatctgcgg ccagtgatgc cagagaaaaa
     7441 accccgtcac gctgccaacc aggatggttt tcaaaattgc tttaagcaac tccgcgccag
     7501 tctgtgccga gaacatccgt ttaatgcccg gtagcgggtt gagtttggaa aacttcggct
     7561 gcaaggattt tccgctaaat accagccccc ccagcatgac cggagaaatg agcgctacca
     7621 gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc agcagaataa
     7681 tctgcctgag gatcagattc gggtctttga taatactgtg atcaaaatgc agcccagcgg
     7741 agagcatgcc cgacaatcga cgggccagcg acacaccgcc gaaccagata acacacacgc
     7801 ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttag ggcttttgct
     7861 gccacacatc aagcatagtg tgcgtttgtc ggatgcggcg tcatcgcctt atcagaccgc
     7921 ctgatatgac gtggtcacgc cacatcaggc aatacaaatc aaaatccaag actatccaac
     7981 aaatcgtcca cctgatcctg actggctacc acaccggctt tgctggtatc gacctgaggt
     8041 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
     8101 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
     8161 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
     8221 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
     8281 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
     8341 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
     8401 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
     8461 atggcttccg ccgcttcggc aatggcctga tccagcccca gttcccgcaa actgtcgcgc
     8521 agcatacgcg tcaggctgcc gatgcgcgca attatatcgc cagctgaatg ctcgtcagca
     8581 ggtttgattg atggttgcat catagtcgca tcctcacatg tccagtttat caaagatttt
     8641 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     8701 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     8761 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     8821 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     8881 gtcgacgcca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     8941 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     9001 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaatttt tgccaacatt
     9061 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     9121 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     9181 tgacgcatcg ccaacattcc caccgcgccg tcgttgccca taccggtcag gatcacccca
     9241 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     9301 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     9361 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     9421 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     9481 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     9541 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     9601 tcagaactca acaacggacc cgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     9661 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     9721 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     9781 gcgcgcagcg tgacttctga tcctttgctg gtcaaggaag aaaccatcac aacgggcatt
     9841 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     9901 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcgac cagcggatca
     9961 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
    10021 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
    10081 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcaaggtgg ctaaagtttt
    10141 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
    10201 tctcctgctg ggtattttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
    10261 acggccccgg cacggtgtac tgtttcgcca gtagattcag cggggcaaaa tcaacatagt
    10321 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
    10381 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgataa ataccgcttc
    10441 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggcg
    10501 cggtgcccaa tgtgtcagcc agcgtcatcg caatgctgta cggctcttcg ccggttgaag
    10561 ccgccgcgct ccatacgcga tactcgccag aacggcgacg tgcgtgatcc gcgagcagag
    10621 ggaaatgatg tgcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
    10681 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
    10741 tcagtcccag cgaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatgat
    10801 cggccaggac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
    10861 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
    10921 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
    10981 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
    11041 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc tgcttcttct accagcgagg
    11101 cgttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
    11161 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
    11221 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
    11281 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
    11341 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
    11401 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
    11461 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
    11521 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
    11581 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
    11641 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
    11701 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
    11761 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gctaccaata
    11821 atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct gatgtagatt
    11881 gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt acgctgactt
    11941 tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc aaagttaacg
    12001 tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag ttgattactt
    12061 tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact tttctccgtc
    12121 tctttttgca atgccctgac gtgctcgttg cccgtagtca tcgccataaa acttttaaac
    12181 agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt aatgtcatcc
    12241 gccggatagc taagtgccgt cagagtgccc gctttgttca gcgcggtact ggcctgtaac
    12301 attatcgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc gacctgattc
    12361 agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc aatctgcaag
    12421 atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg attaaacatc
    12481 aacgctcccc atcaggcggc aatgaccgcg ttagtaaata ctcgtcaaaa tgtttcctag
    12541 tttggatctt gttcagcaat tcgcagtcgt ggctgtgccg gtggttgctc actggcagga
    12601 cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa cgcggaaacc
    12661 gcttgcgtta aacgactcgc ctgttcttcc agcgcagcgg cggcggcagc tgattcctgc
    12721 accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa tgcgacttga
    12781 acgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat gtcagtcacg
    12841 cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac cagcaccgaa
    12901 ccggtatcaa cgcgtgcgac ggagtcttca atgagggctt tgatctcttt tgccgcctgg
    12961 acgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc acggccctgt
    13021 tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt ctggaaggca
    13081 ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc gatctcatgc
    13141 atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc ggaggcactt
    13201 tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt cgcggtgagc
    13261 tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt acgggaggaa
    13321 agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc tgaaccttcg
    13381 cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct ctgcgccagg
    13441 tcgcccattt cactgcgccc gtcaatgttt tgcattccct gggttggctg agcgaaataa
    13501 gctccagtat tgccataatc tagataatca atcagttcag ttaacgctgt gtaatagttt
    13561 ttatagtttt tatatttttc atcaatatta cgactggtag cgaccatttc aggtaacggt
    13621 gccatgcttt tgaatttttt ataatgcgtc gctgcctgcg ccaatgtttt cctggcgcta
    13681 tcgagcaatt caactttggc gttactttgt tgattggagg aatccatcat catccgcacc
    13741 gctgaacgac tcaggttaat gcgcgtttgc agcattaaat cccaggttga cgtcagctcg
    13801 ccctgctgtt cccgcaattg attggaaacc acaaagctct tctggctatg gtgaagggaa
    13861 gaaaaaaaca gactgccgga aataagctgt aacagtgcaa atacccccaa caccattacc
    13921 aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa caagttgatc
    13981 tcgttatcgg caagaagggg ggaaacttta ttgctgatgc cacccgccgc gaaattgaaa
    14041 taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg ggagaattac
    14101 gccacttctg acgccgcact atctaacagc gccatctctt cgctgttcag cagtttttcg
    14161 atgttcacca gaatcaacat ccggtcgccc agtgcgccca gtccagtgag atattctgtt
    14221 gaaagcgtca cggcaaattc cggtgccgga cgaatttgct ccgccgtcaa tgaaagcacg
    14281 tctgagacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag gacgataact
    14341 accgtgttgt cgttatagtc cacatccact tggctgaact taattcgtaa gtcaacaatc
    14401 agcacaataa cgcctcgcag attcgtgacg cctttgataa acgctggcgt gttcgcaatc
    14461 cgtgttacct gatcgtagcc acagatctcc tgctctttca ggatatcaat accgtactct
    14521 tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc cagctttgtt
    14581 acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg gtgttcgcca
    14641 tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt gccacgctgc
    14701 cgtcgccaag aatggtcgca gcagaaatgc cggggacttt gcgatagtta ctttcaaggt
    14761 ttttaaccac aacctggtgc tgaccaatta attgatccac cagcaaggcg tagcggcgac
    14821 cgccactttg taagatcacc acaattccct gggtggcttc ggttttctcg cccgcgacgt
    14881 tgaacacttt ccacagttcg acgatgggca gatattcgcc ccgcacttcc agcacccgct
    14941 cgccgccggc cagtggatgc agatcggctt catggggttg cagtgattcc atgacagcat
    15001 tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg aggatggcca
    15061 gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac tggatttcga
    15121 catgaccgcc catctcctgg atattacgtt taacgacgtc catgccgacg ccgcgcccgg
    15181 agacgtcggt gacctgctct gccgtggaga agcctggtgc aaatatcagc atcgcgactt
    15241 cgtcgtcgct catgttttcg ctgacagtca aaccttgcga ggccgctttt gccagaattc
    15301 gctcacggtt tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg ccgccctgat
    15361 gttcggcaga cagaattaaa tttccgacgc tgtttttacc tgcggcgagc cgtttttctg
    15421 gcagttcaat accgtgatcg aggctattgc gtaccaggtg ggtcagcggg tcgataatgc
    15481 gttctatcag gctcttgtcg agctcagtgg aactgcccac cagcgtcagt tctacctgct
    15541 tgccgagttt tcccgccaga tcacgcacca gccggggata gcgactaaaa acatattcca
    15601 tcggcatcat gcgaatcgac atcactgatt cctgcaaatc acgggcgtta cgttgtaact
    15661 gccccatgct ggttatcaaa tcaccatgat taaccgggtc cagttcgctg gaacgctggg
    15721 caagcatgga ctgggtgata accagctcgc cgacgaggtt aattaattga tcaacctttt
    15781 ctaccgctac acggatgctg gtggatttac tgctgcgcgt cgttttttcc cgctccacgc
    15841 ggccggttgg tgcttgttcg gctgccagtt taagcactgg tggggtggat atttttggcg
    15901 agacttctac tgtttcaaag gtaatctgat cggcttcaat cacaaaacag agtaccgctg
    15961 tgatgtcatc ttcggcgatg tcgcccggta atattgccga gagcgaatcc gcccctttca
    16021 ccacgtcagt taatgtcggt gttgagttgc atctcacctc gtctggcttc atcgagcagg
    16081 ttttccatca gatgcgtggt ttcctgcaaa acgctgaagc caaaagttcc tgcccctcct
    16141 ttgatcgagt gggcagcccg aaagatggca ttcaattgtt cggcatctgg cgcttccggc
    16201 tgcaaaacca gcagatgctg ctccatatca gccaacagtt cgtccgcttc atcaaaaaat
    16261 gtctgataaa aatcgcttat atccatgctc acgctgtcac ctcggttcgg ctgatggcat
    16321 tgtgggaaca ctgacctgtg gtgcaacctc aggtttttcc agggcgctta ctggctcatt
    16381 ctggctttcg gcgttttcat gcaaaatggc ctgttcggct tgtttgttca gtaccagcag
    16441 gctgatgcga cggttgacgg catcatcagg tccgcgatcg cttaagcgca tcgttgccgc
    16501 catgccgacg acacgtaaca ctttgccgct atccaaccct ccgaccatca gttcgcggcg
    16561 ggatgcattg gcccgatcgg cagaaagctc ccagttgctg taacctttct caccactggc
    16621 gtaggggaaa tcatcggtat gacctgaaag gctaatacgg ttgggaatac cgttcagtac
    16681 aggcgcgatg gcacgcagaa tgtcgcgcat atagggttcg acatcggcac tgccggttct
    16741 aaacatcggg cgattctggc tatcgatgat ctgaatacgt agaccttcct ggaccagatc
    16801 gattttgaga tggggacgta acgcccgcag tttcggatcg gactctatca actggtcgag
    16861 atcaccccgc aatttccgca atcgactttg ctccatgcgt tttttcagct cttcgatgtt
    16921 cggctgctta ttcacttccc cctggctttg ggtgtaatca tcaccaccgc cgggaattgg
    16981 gctttcacta ttagaaatgc gatcgccgcc cgtaaccgca gtcgccagtg gagtccggaa
    17041 gtactccgca atctgaatca gctcttttgg gctggagatg gagatcagcc acatcaccag
    17101 aaaaaaggcc atcatcgcag tcataaaatc ggcataagca atcttccacg atccatgtgc
    17161 tgccccgtgg cttttggctt tgcgtcgttt gacgacaata atcggatgcg tttgattctt
    17221 catgcttcct cggttgtcgt ctgttgttgc ggatttttca ccgcacgcac atgctcttcc
    17281 agttcaataa acgacggacg ttcgctggaa tagagcgttt tgcgaccaaa ctcaacggcg
    17341 ataggcggtg cgtaaccgtt cagattagaa agcagagtga ctttgacgca ctgcatcatt
    17401 ttgctggttt cggcgctttt ctgacgtaaa acactcgcta gtggggaaat aaatccgtaa
    17461 gccaataaaa taccgaggaa agtccccacc atcgcatgtg cgataagcgc ccccagctcg
    17521 gcggcaggac gatcggctga acctaacgcg tgaacgaccc ccattacagc cgcaacaata
    17581 ccaaacgccg gaagtgagtc cccgaccagc gccagactgt tcgccgggac ttctgcctcg
    17641 ctttcgtgcg tctcaatctc ttcatccatc agggcttcga tttcgaaggt gttcatgtga
    17701 ccgctgataa tcaggcgcag acaatcgacg ataaaatcaa gcatgacgct atccgcgagg
    17761 atgcgtgggt agctggcgaa gatctcgctc tcacggggat tttcaatatc acgttccagc
    17821 gaaaacatcc ccatctgccg cgatttcgcc atcaaccgat aaagcagagc cagcagatcc
    17881 atatacattg ctttggtgta tttggagcga cgaaacagca acggcagcgc tttcagcgtg
    17941 cctttaatcg ctttgccatt attgccgacg ataaacgacc caatccctgc accggcaata
    18001 atcaccagtt cagcgggttg atagagtgct ccaaggcttc caccggtcat caaataaccg
    18061 ccgaaaactg taccgagaac aaccaggtaa cctaataaga taagcacgac atcatccttc
    18121 cactgttgac catgacagga cgttcagtcg tcaggcgtta acgcgcgatt ggggcaaaaa
    18181 aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc cacaccgtat cagttaaaca
    18241 gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt ctgggaaagt
    18301 ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa gctgccaaca
    18361 ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga aagttgcagt
    18421 aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag cagtggtcct
    18481 tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac cgcatcgacg
    18541 ccattacaca aacctgtttt cagtaaaaac tgccatgcat tacagaacat cgaagcatga
    18601 acgttttgtt cctaggtcat aaaccagtcg gttgagaatg gcagcatgcc tttcggcggt
    18661 gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa ctgtgtttcg
    18721 ctttccagca tctgcaaacg agcgcccagg gtgatcaatt ccattgccag ctgaatatcc
    18781 cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttgcag cgcttcttca
    18841 ggctgattaa catcattcag caagcgtgtt gagagcatga tgccggtatg aatttgctgg
    18901 agatcgttaa cgcgggaatc ttgcgtcaac tgagtaatcg tctggtggct gtcaaaacgg
    18961 aagtgacaaa ccagttgatt ggtttctgcc agcttaacca tttgcggaag agtcagtgcc
    19021 gctaacgttg tcgccatttc ttcatttatg ccgagacgaa acatagcgga cgctttgtcc
    19081 tgaacaatca aacgctgtgc aagtagtaaa tatgacaagt tgatgtcata aatgtgtttc
    19141 agcaactcgg aggtatgcat tattcccacc cagaataacc aactttattt ttatgcggtt
    19201 tcaccgcacc ccgtgatgtc gccgggaagc cccggtaaaa aataattagc attagaatag
    19261 ttgcgataag ctgcaataag cagaaccacc tttttggttt aatatgtcct tacaaataga
    19321 aatgggtctt tacacttatc taagattttt cctaaatcga cgcaactgta ctcgtcacta
    19381 cacgcacata caacggaggg gggctgcgat tttcaataat gtgtgatgca gatcacacaa
    19441 aacactcaat tacttaacat aaatgcataa gccatacttt tgttttgggt gtatttcaat
    19501 ctgttaaaaa gtttttcgct acgctagcaa gcaaaaatga aactggaata atcgaaatgg
    19561 gatgttgcgc acagttaaaa taactcaccg taaataatca tctgctataa ataatcactt
    19621 tcatgcaata ccagataagc tatttttaaa cagacactta ccgcacaaca aactaattaa
    19681 atacaaagaa aggcaacaat caacttaatg ttaatgaaat gttttacata tattaaccac
    19741 ttgtttaatg cggagaatca gtacactatt ttcttataat tacatttgaa attatatgat
    19801 cacatggagt gattaattaa ttgtctttac taataatcag gctaatattt acctgtttga
    19861 ccgcgttggg attgcgtcgt ttctccatta ggagtaaagc tttaatgtca cctgaagttc
    19921 acagaataag gaacaggtta tgagctatag caatattctt gtcgctgttg cggttacacc
    19981 ggaaagtcag caactgctgg caaaagcggt atctatcgcc aggccagtaa agggacacat
    20041 cagtttaatt actctcgctt ccgacccgga aatgtacaat caattagccg cgccgatgct
    20101 tgaagattta cgcagcgtga tgcaagaaga aacgcaaagc tttcttgata agttaattca
    20161 ggatgcgggg tatcccgttg acaagacgtt tattgcctac ggtgagttaa gcgaacatat
    20221 tctggaagta tgtcacaaac atcatttcga tttggtgatt tgcggtaatc acaatcacag
    20281 tttcttttcg cgagcatcct gctcggcgaa aagagtgatt gcctcaagtg aggtcgatgt
    20341 gctgttagtt ccacttacgg gtgattaacc gctcctacgc aagctttgga aaggtagcaa
    20401 ctttatcgcg ctgctggctt tccgcgcttc gcggaactat ctgctttagg tcgctaatga
    20461 agcactcctg ccagtggtta atatcgtttt tcacgataac gtccagcatt tctgcatgac
    20521 gggaaatacg ttccgccagc gacatagtca atgcacgatc cagcgcagct gcaacttcgt
    20581 cacgatcgta ggggttaaca attaacgccg acgttaactc gtttgccgct cccgcaaatt
    20641 gcgaaagaac aagaacgccc gggttggctg ggtcctgagc agcaacatac tcttttgcta
    20701 ccaggttcat cccgtcacgc agtggcgtca ctaagcccac gtcagagtag cggaatattt
    20761 tcatcagtaa tttacggtca aaatgctgat tcaaataata aagcggcgtc cagcctaatt
    20821 gcccgtattt accattaatt cgtccagctt cattttcgag ctgatgacga atatcctgat
    20881 aggcttgcac atcaccacgc gacgttggtg caatctgggt
//
LOCUS       DAJTNA010000062        20603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTNA010000062 DAJTNA010000000
VERSION     DAJTNA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20603
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.063"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(100..732)
                     /locus_tag="OO161_001907"
     CDS             complement(100..732)
                     /locus_tag="OO161_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5462985.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1028..1303
                     /locus_tag="OO161_001908"
     CDS             1028..1303
                     /locus_tag="OO161_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5462986.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAIWFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(1380..1622)
                     /locus_tag="OO161_001909"
     CDS             complement(1380..1622)
                     /locus_tag="OO161_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5462987.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            1975..2895
                     /gene="lpxP"
                     /locus_tag="OO161_001910"
     CDS             1975..2895
                     /gene="lpxP"
                     /locus_tag="OO161_001910"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5462988.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEHEKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALEKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            3251..3322
                     /gene="ypdK"
                     /locus_tag="OO161_001911"
     CDS             3251..3322
                     /gene="ypdK"
                     /locus_tag="OO161_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5462989.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3387..4625)
                     /gene="alaC"
                     /locus_tag="OO161_001912"
     CDS             complement(3387..4625)
                     /gene="alaC"
                     /locus_tag="OO161_001912"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5462990.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            5002..6699
                     /gene="ypdA"
                     /locus_tag="OO161_001913"
     CDS             5002..6699
                     /gene="ypdA"
                     /locus_tag="OO161_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR5462991.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            6714..7448
                     /gene="ypdB"
                     /locus_tag="OO161_001914"
     CDS             6714..7448
                     /gene="ypdB"
                     /locus_tag="OO161_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5462992.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            7577..8314
                     /locus_tag="OO161_001915"
     CDS             7577..8314
                     /locus_tag="OO161_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5462993.1"
                     /translation="MGSLCIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAAN
                     LPINNKPVMLLSLVFAPTWLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTH
                     LSRSPLEQNIIQPLVLSLLHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTR
                     ESVAQFFNITPNHLSKLFAQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGF
                     PDSDYFCRVFRRQFGLTPGEYSARFQG"
     gene            complement(8317..10812)
                     /gene="ptsP"
                     /locus_tag="OO161_001916"
     CDS             complement(8317..10812)
                     /gene="ptsP"
                     /locus_tag="OO161_001916"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5462994.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRCLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSGIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGCTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEALG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQFRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(10837..11874)
                     /gene="ypdE"
                     /locus_tag="OO161_001917"
                     /pseudo
     CDS             complement(10837..11874)
                     /gene="ypdE"
                     /locus_tag="OO161_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(11874..12959)
                     /gene="ypdF"
                     /locus_tag="OO161_001918"
     CDS             complement(11874..12959)
                     /gene="ypdF"
                     /locus_tag="OO161_001918"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5462995.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(12974..14221)
                     /locus_tag="OO161_001919"
     CDS             complement(12974..14221)
                     /locus_tag="OO161_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5462996.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(14243..14569)
                     /locus_tag="OO161_001920"
     CDS             complement(14243..14569)
                     /locus_tag="OO161_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR5462997.1"
                     /translation="MSKKLIALCACPTGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(14788..15753)
                     /gene="glk"
                     /locus_tag="OO161_001921"
     CDS             complement(14788..15753)
                     /gene="glk"
                     /locus_tag="OO161_001921"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5462998.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            15957..17213
                     /locus_tag="OO161_001922"
     CDS             15957..17213
                     /locus_tag="OO161_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5462999.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAGGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            17328..17654
                     /locus_tag="OO161_001923"
     CDS             17328..17654
                     /locus_tag="OO161_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5463000.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(17795..19033)
                     /locus_tag="OO161_001924"
     CDS             complement(17795..19033)
                     /locus_tag="OO161_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5463001.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            19369..20571
                     /gene="nupC"
                     /locus_tag="OO161_001925"
     CDS             19369..20571
                     /gene="nupC"
                     /locus_tag="OO161_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5463002.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVV"
BASE COUNT         5020 a         5443 c         5066 g         5074 t
ORIGIN      
        1 ccaaataatt ttatgaccac aacctgtcgt attgtggtca taaaaaattg tcggctggag
       61 ggagttaaat cctttacctc aatatactgc ctgtcagcat tagttcacaa acagtgcttc
      121 gctgtcgacg atgattccat cttccgcacc tttaagcgcc aggtttgcct cgtagtattg
      181 ctttttatca agcagtttct gcgcatcagc aagtgcatta cgagtctgtt taagcggcat
      241 cagatattgg ttttccatta cgccaacacc ggccaggcga agttcttcca ttgcgccctt
      301 tttatcccct ttcgccattt tttcattggc aatttttatc gcggcttctt tttcgggagt
      361 tgctacataa ctctcagaaa ttccgacaga tgcattaatg acaatatatt ggtcatcatt
      421 gagattggtc tttttacctg gcttagcgaa ctttgcccat tcggtactgt catctgacag
      481 caaagcggaa gcttcattgg tcagttcttt tgccttttct ggatcgccat gaaacagagc
      541 caggcgcgct acctgcacgt cacgcatcgc atataaacct tgttcagaga tacgctctgc
      601 tgctgccgtt ttctgggttt gctgaacttg ctgagtttgc tgttgtgccg ctaccggtgc
      661 attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg tagccataat
      721 taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc atcatattgg
      781 gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta tcttacagtt
      841 gtagttatta tcgcaagaag tattttagtc ttacaattgt aggaatctgc ttcctgtcgc
      901 agagagctat tcttatgtac cacaaagaga tgtcatttat tatatcgatg taaatattga
      961 ttaattatat gtaaatacag ccataacatc gtaggttctc agtgaaccat tttcaggata
     1021 actttctatg aaagtaaact taatactttt cagcttattt ttattggtct ctattatggc
     1081 atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct ataaagagac
     1141 tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat cacaggcgat
     1201 ttggtttaag atgagagaag atgcgccacc attaaacctc acagaagaaa tcacgcctac
     1261 ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt cacatggcag
     1321 gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat cctgacccac
     1381 tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg ggcaagaaac
     1441 ataaatatcc ccgtcactag ttcattaccg ccagttaacc acatggtgaa gtaataaatg
     1501 agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac gttcagtacc
     1561 tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca taaataaatc
     1621 atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta atagtaagac
     1681 aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcatgt taaatgcaag
     1741 actgttgtgt acggaaaaat atttactttg cacgattaat aatcagtagc tgaaagcagt
     1801 cagcgagtga taaagtacag ataccgcatt catgctcctc gtggttatgt cctgactagt
     1861 ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt gagtgtaccg
     1921 ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt aactatgttt
     1981 ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac atggtttggg
     2041 cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt tctcggcacg
     2101 cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc ccgtaaaaac
     2161 ctggaacttt gtttcccgca gcattctgcg gaagaacacg agaagatgat tgccgaaaac
     2221 tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg gcccgacagt
     2281 cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc acaaatgcaa
     2341 aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg cggccgcgtg
     2401 atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca gctgatggaa
     2461 tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag aaataatctg
     2521 cgcggcattg tcggtgcact ggagaaaggt gaagcggtat ggtttgctcc cgatcaggat
     2581 tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt cgccacaacc
     2641 aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt aacgatggta
     2701 agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga aggctacccg
     2761 acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga gatcatgcgc
     2821 gcaccggagc agtacctctg gatccactgt cgctttaaaa cgcgcccggt gggagaatca
     2881 tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc cataataacc
     2941 tcatagtttt tatggctttt ttcttaaggt tttttccttc gctgaatatc taattattac
     3001 cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa gtaaataaga
     3061 aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtcttg agcagtttag
     3121 ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc gctttgcccg
     3181 gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagca attacgcgga
     3241 gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg tttgggccgg
     3301 tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag ccatcaggct
     3361 cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg tttgctgctg
     3421 gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc ctgacgaata
     3481 cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc gccaaagcca
     3541 atccccggcg agacacagac cttcgcttcg ttaagcaatt tcttggcaaa taccagcgag
     3601 cccatggcag catacggttc cggtattttc gcccagacat acatcgaagc cttcggcatt
     3661 tcaactatcc agcccgcttc atgcagccct ttaaccaata catcacggcg gcgtttgtac
     3721 tgttcggcaa tgtcgcgcac gcactgttga tcgccctcca gcgccgcaat cgccgccacc
     3781 tgcaacggcg taaaggtgcc gtaatcgtga tagcttttaa tacgagccag ggcgctgacc
     3841 agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta gcttttcgac
     3901 agcgtaaaga actcgactgc cacatcgcgt gcacccggta cctgcatgat tgacggcgct
     3961 ttccagccat cgtagacgat atcggcatag gccaggtcgt ggaccaccag cacatcgtag
     4021 cgtttcgcca gcgccacaac cttttcgaag aactccagct ccacgcattg cgcggttgga
     4081 ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg aatggcgcgc
     4141 tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac ctgcgccccg
     4201 gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag caccgtgtca
     4261 ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc aatagtgacg
     4321 atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca gcgggaaatg
     4381 gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc cgggcgctgg
     4441 gccacagtac ataatttttc gacgatatgc ggtggagtcg caccgtccgg gttacccatg
     4501 ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc agcggtgatg
     4561 ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg gcgagtgtca
     4621 gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc tgccacaatg
     4681 gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta gaacattttt
     4741 atcaatggct ttaaatgagg aactccactt caaaaacagg aaatttttct acgctaacaa
     4801 gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag ccagaagtgc
     4861 attatcaaga gatacattgc taatacacca tttatctttc aatccccttt taaatttatg
     4921 gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc atagaggttt
     4981 aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc ggtcttcgat
     5041 cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct gtttcgcgag
     5101 ctgttgcaca agtcggcaca ctccccaaaa gagttgcttg ctgttaccgc cattttctcg
     5161 ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct ggtgaacgta
     5221 cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg catcattacc
     5281 ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac ggcgatcccc
     5341 tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct gaaaatcccc
     5401 aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac gttgaccatg
     5461 attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt ctctaaaatc
     5521 ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct ggtgcaaagc
     5581 gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct ggatatcgcc
     5641 aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa ggtctgcgaa
     5701 attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga tcatgtgctg
     5761 gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat tagcccgact
     5821 acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga agcccaccgc
     5881 acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggcgt tgtgaccgga
     5941 acgctgaaaa tttactactg ccacgcgcat cagataacct cgtcattaca ggaaatggcg
     6001 gttggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga gcagctacgt
     6061 gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc ccattttctg
     6121 tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac cgctcgccag
     6181 ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga cgatgagcaa
     6241 atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga gcaggcccgc
     6301 tttggtgaca agctgacggt tatctatgat attgatgaag aggtgaattg ctgcattccc
     6361 agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca gccttgcaaa
     6421 ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg cattgcggtg
     6481 cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc gaatgaaatg
     6541 ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt gtatggcgag
     6601 gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat tcctaaccaa
     6661 cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccgaagtgat attgtgaaag
     6721 tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta attaaagagc
     6781 acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg aagtttttgc
     6841 agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg gatggcgtgt
     6901 tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc atcaccgcgt
     6961 ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt ctcaaaccgt
     7021 atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg caacaacagc
     7081 agaccagcag tacgcctgca gcgacggtaa cgcgtgagaa tgacaccatt aatctggtga
     7141 aagatgagcg aataatcgtc acgccaatta acgatatcta ttacgccgaa gcgcacgaga
     7201 aaatgacctt tgtctatacg cggcgtgaat cctacgtaat gccgatgaac attaccgaat
     7261 tttgcagcaa actgccgccg tcgcattttt tccgctgcca tcgctcattt tgtgtcaatc
     7321 tgaacaaaat acgcgaaatc gaaccgtggt ttaataacac ctacattctg cgactgaaag
     7381 atctggattt tgaagtgccg gtcagccgca gcaaagtgaa agaatttcgc cagttaatgc
     7441 atctttaatc gggaactttc atgaaagcac caggcttgcc cgccgatcaa caatttttcg
     7501 ccgatctgtt cagcggcctg gtgcttaacc cgcaactact ggggcgcgtc tggtttgcca
     7561 gccagcctcg ttaccggtgg gcagtttatg tattgatttt ccccgtctgg atatcgtgct
     7621 gcgcggcgaa tacggcaacc tgctggaagc aaagcagcag cgaatggtgg aaggagaaat
     7681 gctgtttatt ccggcacgcg cggctaattt accgatcaac aacaaaccgg tgatgctgtt
     7741 aagcctggtg ttcgcaccga cctggcttgg gttatcgttt tacgatagcc gcaccacgtc
     7801 gttgttgcat cctgctcgcc agatccagct tcccagcctg caacgcggtg aaggtgaagc
     7861 gatgcttacc gccctcactc atcttagccg ttcgccgctg gagcagaata tcattcagcc
     7921 actggtgtta agtttgctgc atctttgccg caacgtggtg aatatgccgc cgggtaattc
     7981 gcaaccgcgc ggcgattttc tctatcacag catttgtaac tgggttcagg ataattatac
     8041 ccagccgctc acccgcgaga gcgtggcgca gttttttaat atcacgccca atcatctgtc
     8101 aaaactgttt gctcagcatg gaacgatgcg ttttatcgag tatgtgcgtt gggtgcgaat
     8161 ggcgaaggcg aggatgattt tgcagaaata tcatctgtca attcatgaag tggcacagcg
     8221 ttgcggtttt ccggatagcg actatttttg tcgcgttttc cggcgtcagt ttggtctgac
     8281 gccgggagag tacagcgccc gttttcaggg ctaacgtcag aaggttaatt ctgtttccag
     8341 cagcgtcagg atactttgtg catcttgcgc ggcaaacaac gactggcgga agtttttatt
     8401 caccagttta cgcgccagct gcgagaagac tttcacatga ttcatccctt cgttagcacc
     8461 cagcgtcagc atgatcacca gttcgacttc gcccatttct gactgccagt caatcggttt
     8521 cgccagccgg gcaatgctga tactggaatg acggatccac tgagatttgg tgtgcgggat
     8581 cgctacgcca aaaccaacgc cggtggtaac aatctcttcc cgctgccaga catcttcttc
     8641 cagctcaaac ggatgttcag tgcgcccgtt aacgccgagg ttgccgcaca ggaactggat
     8701 cgcctgctct ttattgctaa aatcctgatc aacaaagata ttctccagcg ccagcagtgg
     8761 gcgaacgtct tcttccggcg taaaggcggt gagtaacgct tcaatttcct gcgcactgcg
     8821 gcattcacat gcctgacgcg ccagttcccg acacgcctcg ctatccagtt gacgaaactg
     8881 gcttttcacc gccggaatac gcgggctact catactcagc tcatccaggc ccagcccaag
     8941 cagtagcggc agataacggc tttcaccgcc cagttcaccg caaatgccta cccatttgcc
     9001 ccgctgatgc gcagtggtaa ctatttgctg caacatgcgc aggaacgatg gcgtaatcgg
     9061 gttatatagc ggcgatacgc gcgggttatt acgatcgacc gcatacagat actgggtcat
     9121 atcgttggag ccgatactga agaaatcgac ctcatcgcag aagtggtcga tgatgtagca
     9181 caccgacggc acttccacca tgatcccaag cgtaatcgtc tctgcatgac gcaggccatc
     9241 gcgcttaagc tcaacgatcg ctttttggat ctcgcccttc acccataaga tctgatcgag
     9301 actgtggacc atcgggatca tcaactgggc gttgccaaaa ctggcggcgc gcaaaatcgc
     9361 ccgtagttga gtgcggaaca ggccagcaaa ttccgggtaa atacgcaccg cgcgatagcc
     9421 gaggaacggg ttctcttcct ggggaatatt cagataagga atgcttttat cgccaccgat
     9481 gtccattgtg cggaagataa tcggcttgtc gcccgctgcc agtagcacct gctggtaggc
     9541 ttcaaattgc tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcagtacg
     9601 gaacagtcca acgccttccg cgccgttggc aaacgcgcct agcgcttcca gagcggtgcc
     9661 gatattcgcc gcaatatcaa tacgcttgtt atcacgggaa taggccagct gcgcggcggc
     9721 ctgcgcctgt tgtttttggc gtttatccgc cagcgtctgt gcgacctgat aataaccgct
     9781 caccgcgtca ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca ccgccggttg
     9841 cccggcataa cgggcaatcg catccagcgg caggccgctc agtaccggga tcgccgaggc
     9901 gcgggccaga atcagggtat gcgaggtgca cccagttttc tccagaatca tgcctgcaag
     9961 attttttaaa tcgaggctca aaaactggct tggggttaaa tcctcggcta ccagaatagt
    10021 cggtttttcc agcaccagat tgttgcgcgg cttcagttcc ggccaggtga tatgcagcaa
    10081 ctgttcgcta atatcgcgaa tgccgctaac acgttcgcgc agataatcac tggcagaagc
    10141 agaaagtttg gcgcaaacct gctccatatt gctgatgatc gccgccccca gcccttgatg
    10201 ctgttctgtc atcagacaac ggatattgcc cgcgaattca tcatcctgaa tcagcgacaa
    10261 atgggcgcta aggatagttt tgctttcgcc gtcacgctca cgcaattgct ggttcagttg
    10321 ctcggcaagc gttgcaaggc tgtgctccag ccgggtggaa tcttgcgcac tggcggggat
    10381 tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca cgccgacgct
    10441 gcttgccagc acattgccgt acagtaaatc cgggttcagg cggcttaatg aacgcggcag
    10501 cggatgcgcc gtcagttctg cctgcgttgg ctggatgctg tcgctgtcga taaagcgcac
    10561 ctggatgtac tcttccaaca cgcgctgcgc ctgctcttca tcgctgccgc taatgttcag
    10621 gctgcaactg tcattaaata aagtaccggt gccaatcagc gccagcgagc ttttcgcatc
    10681 ggcctttgcg ttctggcgat ggttgataaa ggtgatttcg ctttgccact ggctgcactg
    10741 ttctttaagt tcccacgccg gacgagcatg aagaccgttt ggcaaaggac agagaaattg
    10801 aatcgttaac ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac
    10861 aaccgtctca cgcgtaagac gtttaataag ggcagataaa agctgctgca tctgcaaaat
    10921 gtcacggcaa tcggcaatcg atgcggcgca atgtccgtgg cgggttgctg gccccatcac
    10981 cactgtaggc acgccggtgc cggttaagtg caccgctccg ccgtccgtgc cgccgttgct
    11041 gaacatatct gcctgtaacg gcacgccaat ttctgccgcc acggtttaga tccacgcggt
    11101 aagttttggc ggcgcaatca gcgacttgtc gcttaacacc agcatcggcc cgttacaaat
    11161 ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg tatcaagcac
    11221 aatggcgaca tccggcgaca ccgcgcgggt ggcagtttgc ccgccgcgta cccccacctc
    11281 ttcgctggaa ctggccacca gccacacttc cgcaggcagt tcagcgctgt gtagttcgcg
    11341 cagtaacgtc accagcaggt agcaaccgag gcggtcatca aaggctttcc ccatcacccg
    11401 ctggtgaggg agaacctgaa aagttgtatc aaaggtgacg cgatcgcctg gacgaattcc
    11461 cgcctgcatc acttcgtcat acgagcgcgt accaatgtcc acgcgcatgg cgctgacgtc
    11521 attcccctgc cggtcgccgt caagcaggcc tggaattttg cactcttcac gggtggtgat
    11581 gcgcaccggc tgcagctggc gggcagccat gcgcacgttg ccaaccggca gcacatcaat
    11641 tgccccttcg cgggagatgc tgcgcaccat aaatcccact tcgtccatat gggcgcagat
    11701 catcaccttc ggccctgtcg attcattgag gcggatcagc accgatccca gaccatcaaa
    11761 tcgcacttct ttttgcaggc gatccgcttc ttccagcagg atctgccgca cttcctgttc
    11821 cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat agcgataaat ccattatgcc
    11881 tctcccgtga gcaacactgt tttcggcatg gcgtagagca cttctgcgcc ttgcggggta
    11941 accagcacaa catcttcgat gcgcacgccc ccttgccctg gcaaataaat ccccggctcc
    12001 acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc gcggtgaaaa acgcggatct
    12061 tcatggactt caatgccgat agcgtgacca gtgttatgac cgaaatagtc gccaaatcca
    12121 gcctcggtaa tgacccggcg cgcggcgtcg tcaatctgct ggcagcgcac gccggggcga
    12181 attgcagaga ttgccgcgag ctgtgcctgc aggacaatct gatagacgtc aaacagcggg
    12241 tgagattcgg cgctcacccc ttcgccattc accagcaagg tgcgcgtcat atcagagcag
    12301 tagccctgat acagcgcacc aaaatcgaga gtgacaaact cgcccgctgc aacaatcttg
    12361 tcgctggctt tgccgtgcgg cagcaccccg cgccagccgc tggcgacaat ggtgtcaaaa
    12421 gaggcttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc tatctcgcgc
    12481 tcgctcatcc ccgcctgaat aaagcggcga atatgctccg ctccgcgatc ggcaatccca
    12541 caggcgaggc ggattatctc cacctcctct ggcgttttga tttgccgcag cacatccggc
    12601 gtggcgctaa ccagtttcgc attgagttca gacttccagc ggtgcgcggt ttcccaactc
    12661 acctgctggc cttcaaaacc gagcgtttgc aactgctcat cggcaatgat ttgattgacg
    12721 atagtggtaa gcgtgttcgt cgcgtcaagc aaatgcagct ggtagccttg cgcacgggct
    12781 tccacctcaa cgaaatagcg cgaatctacc agaatgtgcg cactttcacg gctaataacc
    12841 acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg tgaggagaga
    12901 agcactgcat ccagttgttg cgccttaagc cagtcgcgca gcgaagcgag taatgtcatc
    12961 tggattttcc ttcttacagg ctatcgatta acaatttgcc tttacggaac atcatcagac
    13021 gcaggaacac caccatcagc gcagttatga ctgcccccag cgcgatcccc gccatataga
    13081 cgccaaggtt agtgaccagc ggccatgccc agatagcgga ttccgggaac cattgcactg
    13141 cgcccagcca gacagcggcg gttgagccga caatcgcgcc gaccatatac gacggaatgg
    13201 cggtgatggg gctttccagc gcaaacggaa tcgcaccctc actaatcccc ataaaggcaa
    13261 ggaacatcgc ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt ttgccggtta
    13321 aacgacggtc aataatggtc gccagcccca gaccaatcgg aggaataacg atagcgatag
    13381 aacgagcggt gaccggtaat acgtggtcag tggtaaagct gaaggcaacg aaacctgcgg
    13441 ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca
    13501 tcagcgcgcc cttctcacct gcggcagtca gcacggtacg gataccgccg ttgatccagc
    13561 cgccaaaggg ggtaatgacg tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg
    13621 gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt ttctggttca
    13681 tccacttcac aaggtaaccc gcgacgatag aaataatcag cgcaccaatg aaggtggacg
    13741 gcaccggcga agaggtcgcc cattgcatag tgctgggatc gaagttcagc agttgcgtcg
    13801 gctgggtaga catcaagccg ccgataaaac cagccgggaa cgccagtttg ccaccgatag
    13861 agttcgccac aaaagcagca aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg
    13921 actgcgacag ccaggcaaat ttcagtaagg agaggtcgaa accggagaat ttcccgctat
    13981 taagagcatc catgatgccg atctcagcag gaattttaag ccagctataa gcaatcagct
    14041 ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg
    14101 acatcacatg ctgcgggagt tcaccccaga agctgctttt agaggcctgc ggattcttaa
    14161 ctgccgctgc cgcaccggat gcgccaggca caacggttgc actgcgtttt ttaatggcca
    14221 taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct tcgatttctt
    14281 tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg
    14341 attcgaaacg ctcattatct tccggggtaa ctgccacgga gtggatgatg atggttgctt
    14401 cggcgatatc ctgcgccgtc aggcgattct ggataccgtc cgcgccctgg gtttcaattt
    14461 tcacttcata accggcttct accgccgctt cttccaacgc ctgagcggcc ataaaggtgt
    14521 gagccaggcc cgttgggcag gcacataagg caatcagttt cttactcatc gttaattcct
    14581 cattcagtga tagttgacga gtgatattat gaacgtgcaa agcaaatcgc cgttaccaga
    14641 caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc
    14701 ttgtgagggg acacaaagaa aaattgaatt aactgtatga tttaaaagat tatcgggaga
    14761 gttacctccc gatataacag gaaggattta cagaatgtgc cctaaggtct ggcgtaaatg
    14821 tgcaccggaa ccgagaagac ccggattgtc atggacgatg agatacaccg gaatatcatg
    14881 gacatattct ttaaagcgcc ctttatcttc aaatgcagca cggaaaccag aggctttgaa
    14941 gaactcaagg aagcgcggca cgataccgcc cgcaataaac acgccgccaa atgtcccgag
    15001 attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg acaacgcgcg
    15061 gcggcaatcg gtacagctgt cagccagcgc gcgttcggta atatcttttg gcttgagatt
    15121 ttctggcagg cggttgtcag ctttcacaat tgcgcgatac aaattcacca gcccagggcc
    15181 agaaagcacg cgctccgccg aaacatgacc aatttccgca cgcaatattt cgaggataat
    15241 ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg gcaagcttac
    15301 ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cctgttccgg caccgtaaac
    15361 cgcaataggt ttaccttcta ccggttctgc gccaccaaac tgaatcagat gctctttttt
    15421 cagcatcggg atcgccatcg atacagcggt aaaatcgtta ataatttcca gatggctaaa
    15481 accgagattc tttttcattt cggcaattga gaacgcccag gtatggttag tcattgccac
    15541 ccagtcaccg gtaattgggc aagcgatggc aatacagccg tctttcacct cgaccttatg
    15601 ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc ctgaataggt
    15661 cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg cgttggtgcc
    15721 gcccacatca ccgactaatg catactttat cattcttcaa ctgctccgct aaagtcaaaa
    15781 taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga aaggcaggct
    15841 ccctgtaaat atcgatctgg gtcacacatt tactttatcg tttcagcacc aattgcagcg
    15901 atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa tagaacatgc
    15961 tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca attgggattg
    16021 cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag aatttgctct
    16081 ggcaacgact gccgggaact ctggggatag cccaggattc acctctctgg atcatcggtg
    16141 tattaacgct aacgggcatt gcggtggggt tggttatccg tttcagccag ggtcatgccg
    16201 gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccagt tccgccctct gcgctaccag
    16261 gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc
    16321 cgatcatgac cgtcaatatc gcccttgccg tcgccattgg cgctcgtctg ttaccgcgcg
    16381 tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg ctgtttggca
    16441 cacctgttgc agcggcgttg atattttcgc aaaccttaaa tggcagtagt gaagttccgc
    16501 tatgggatcg tctctttgcg ccgttaatgg cggcagcagg tggtgcacta actaccggat
    16561 tatttttcca tccccatttt tcactgccca ttgcgcatta cggacagatg gaaatgaccg
    16621 atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat
    16681 ggtgcttacc acggttgcac gcgatgatga atcaaatgaa aaatccggtg ctcgtgctgg
    16741 gtattggcgg atttattctc ggtattctgg gggttattgg tggaccagtt tcgctgttta
    16801 aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc agcgattact
    16861 ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg
    16921 gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc
    16981 acgtacccgc cgtaccagcg gcaataaccg tttcttgcgc tattctcggc atcgtgctgg
    17041 tggtaacacg cgatggctgg ttaagtcttt ttatggcggc ggtcgttgta cccaatacca
    17101 cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta gcaggtaagc
    17161 cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataacgtt tagcaaaaaa
    17221 tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct ccccgtaata
    17281 ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg ttcaggtcac
    17341 tgtttctggc ggccgccctg atggcattta ccccgcttgc agcaaacgca ggtgaaatca
    17401 ccctactgcc atcaatcaaa ttacaaattg gcgatcgcga tcattacggt aattactggg
    17461 acggtggtca ctggcgcgac cgtgactact ggcatcgcaa ttatgagtgg cgcaaaaacc
    17521 gttggtggcg tcatgataat ggctatcatc gtggctggga taagcgtaaa gcgtatgagc
    17581 gtggctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg
    17641 gacaccgcca ttaaacacat tcgccaatgg agcacaatgc ctgatgcgac gctagcgcgt
    17701 cttatcaggc ctacaaaccg gatctttaac gcgtagggcg gataaggcgt ttacgccgca
    17761 tccgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc gtccccacca
    17821 acaaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc tgttttacgc
    17881 gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc agcagtggaa
    17941 ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga atccgtgtcg
    18001 gatctaatcc catcagaatg acaataaatg acggcagcat ggtgactgta cgacgcaccc
    18061 acagcgggat atggaagcga atgaacccct gcatcaccac ctgccctgcc agtgtcccca
    18121 ctaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt gccgcagcat
    18181 ggcttaacag cggttgcagc gtcagataag cctcatcaag atcggcaaca ccagtatgac
    18241 cagaaaagtg gaacgccgcc gcagctgtag ccatcatcgc cagattgaca aaaccggcaa
    18301 tagtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga cgcgaaccgc
    18361 catgtaaatg ctgagtgagc gaggagtgca aataaatcac atgcggcata atcgtcgccc
    18421 ctaacacgcc tgctgccagg aagaccgctt ccgaagtagg taaactcggg atcaccattc
    18481 ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg
    18541 caacaaacaa cagtaacccg ccaatcactt tctccagcgg tttttgcccg cgacgttgca
    18601 gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac aacgaaacac
    18661 caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg gttgccatcg
    18721 caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag tgatcgcgaa
    18781 tctgctccgc cagattttta ccggtggcaa tccctagttt ggcagagagg atctgaatca
    18841 gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg aagctggcac
    18901 ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc gcaatgaacg
    18961 caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta ctctcaacgc
    19021 gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat gctatgataa
    19081 acgagaatga ttatcaaatt catttaaatg gattgtggtg atttctgtga tagaccagga
    19141 ttatgactac gaaaagattg atgacttgaa tgttatggtt atgtttaatg ttagcacatt
    19201 tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagat cactattttt
    19261 gaagcctgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct taaccatgtt
    19321 acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat ggaccgagtc
    19381 cttcattttg tactggcact tgccgttgtt gcgattctcg cactgctggt aagcagcgac
    19441 cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt gttactggcg
    19501 tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc cgaaatgttc
    19561 gaaaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag catgaatgat
    19621 caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg
    19681 atcggtattc tccagcacat tcgcgtgttg ccggtgatca tccgcgcaat tggtttcctg
    19741 ctctccaaag tcaacggcat gggcaaactg gaatccttta acgccgtcag ctccctgatt
    19801 ctgggtcagt ctgaaaactt tattgcctat aaagatatcc tcggcaaaat ctcccgtaat
    19861 cgtatgtaca ccatggctgc cacggcaatg tccaccgtgt cgatgtccat cgttggtgca
    19921 tacatgacca tgctggaacc gaaatacgtc gttgctgcgc tggtattgaa catgttcagc
    19981 acctttatcg tgctgtcgct gatcaaccct taccgtgttg atgccagtga agaaaacatt
    20041 cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga atacattctg
    20101 gcaggtttca aagttgccat tatcgttgcc gcgatgctga tcggctttat cgccctgatc
    20161 gctgcgctga acgcactgtt tgccaccgtg actggctggt ttggctacag catctccttc
    20221 cagggcattc tgggctacat cttctatccg attgcatggg tgatgggtgt tccttccagt
    20281 gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga gttcgttgcg
    20341 atgatggatc tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg catcatctct
    20401 gtgttcctgg tttccttcgc taacttctct tcaatcggga ttatcgcagg tgcagttaaa
    20461 ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaaact ggtttacggc
    20521 tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtggtgta agaccataca
    20581 taaaaaagcc ggggataatt ccc
//
LOCUS       DAJTNA010000063        20374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTNA010000063 DAJTNA010000000
VERSION     DAJTNA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20374
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.064"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..670
                     /locus_tag="OO161_001926"
     CDS             <1..670
                     /locus_tag="OO161_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCR5463003.1"
                     /translation="TYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQDL
                     QLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRELM
                     LLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGSE
                     KELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQPKLRGNVQTAQR
                     EVAP"
     gene            667..1755
                     /locus_tag="OO161_001927"
     CDS             667..1755
                     /locus_tag="OO161_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5463004.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLYSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTSTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKSVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(1843..3141)
                     /gene="exuT"
                     /locus_tag="OO161_001928"
     CDS             complement(1843..3141)
                     /gene="exuT"
                     /locus_tag="OO161_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5463005.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATTNGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3624..5036
                     /gene="uxaC"
                     /locus_tag="OO161_001929"
     CDS             3624..5036
                     /gene="uxaC"
                     /locus_tag="OO161_001929"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5463006.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5051..6527
                     /gene="uxaA"
                     /locus_tag="OO161_001930"
                     /pseudo
     CDS             5051..6527
                     /gene="uxaA"
                     /locus_tag="OO161_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6610..7161
                     /locus_tag="OO161_001931"
     CDS             6610..7161
                     /locus_tag="OO161_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5463007.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7166..8410)
                     /gene="sstT"
                     /locus_tag="OO161_001932"
     CDS             complement(7166..8410)
                     /gene="sstT"
                     /locus_tag="OO161_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5463008.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9025..9990)
                     /gene="alx"
                     /locus_tag="OO161_001933"
     CDS             complement(9025..9990)
                     /gene="alx"
                     /locus_tag="OO161_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5463009.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVAEPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(10274..11260)
                     /locus_tag="OO161_001934"
     CDS             complement(10274..11260)
                     /locus_tag="OO161_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5463010.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11339..12022)
                     /locus_tag="OO161_001935"
     CDS             complement(11339..12022)
                     /locus_tag="OO161_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5463011.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNAGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ESGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12099..12602)
                     /locus_tag="OO161_001936"
     CDS             complement(12099..12602)
                     /locus_tag="OO161_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5463012.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVLGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12687..13823
                     /gene="rlmG"
                     /locus_tag="OO161_001937"
     CDS             12687..13823
                     /gene="rlmG"
                     /locus_tag="OO161_001937"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5463013.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQEPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14108..14422
                     /gene="higB"
                     /locus_tag="OO161_001938"
     CDS             14108..14422
                     /gene="higB"
                     /locus_tag="OO161_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5463014.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14419..14835
                     /gene="higA"
                     /locus_tag="OO161_001939"
     CDS             14419..14835
                     /gene="higA"
                     /locus_tag="OO161_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5463015.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(14880..16898)
                     /gene="fadH"
                     /locus_tag="OO161_001940"
     CDS             complement(14880..16898)
                     /gene="fadH"
                     /locus_tag="OO161_001940"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5463016.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASRIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(17124..19475)
                     /gene="ygjK"
                     /locus_tag="OO161_001941"
                     /pseudo
     CDS             complement(17124..19475)
                     /gene="ygjK"
                     /locus_tag="OO161_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19492..20319)
                     /locus_tag="OO161_001942"
     CDS             complement(19492..20319)
                     /locus_tag="OO161_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5463017.1"
                     /translation="MKYGSWFGLIYGEGWTVQADHDGNAWVPDHSWGGFEGGINRFYG
                     GYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGYVIPNTRDIMTALKVQNLSGNFRY
                     SVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMMNGYIQYDLAEGITWMNGLEITDG
                     TGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYA
                     KHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSRFYMKNASHDPSDQIFLMQYFYW"
BASE COUNT         4973 a         5348 c         5339 g         4714 t
ORIGIN      
        1 cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag ccagtggtac
       61 agacgatttg ctgggtgcgg tattggtgga tgtgaaaggc ttctctgaac aggatgaaga
      121 gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc agcttggtca
      181 aagcgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg aggttaacga
      241 cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag ggaatcgtga
      301 attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct ttaactacaa
      361 ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg tggggctgaa
      421 cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta acggcagcga
      481 aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga gtgttgaaaa
      541 gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga cttattcaca
      601 attaccggag ttgaaacagc ctaaattacg tggaaacgtt cagacggcgc aaagggaggt
      661 tgcaccatga gaaatcgatt gattgcggcg atattgggct tgtttggcac gctcactggc
      721 gttcaggcag ctcctgacgt gaccagtgaa attacgtatg atttggcatc tggtagagcg
      781 gattattact tctggaagga tgaagcgtcg gcaggaaata atggatatat gtggtatgaa
      841 tgttcgtatc ctgacctcca acaaacctgt acagctaatg gaaatatatc gacagtacaa
      901 atctatttaa ctgaacaacg cagtgggatg cgttggccgg taaaactcaa aggatttaaa
      961 acagccattg taagtagcga tgaagcgccg ccaggatgca aggggggcaa agggcttcag
     1021 acgaatctta aggattctaa tagatcttca tgtacagaag atggtcaaca ttattatata
     1081 tacgatacaa agtttcttac gctctacctt gagcagacag agatgaagaa tttgccgatt
     1141 ggtggcgtct ggaaggggaa agttaaatta tattcgaaca gcccggccca ggactatttc
     1201 gcaaatatta ccctgaatac gctcgacccc aaccatattg acgtgttctt cccggagttc
     1261 gcccacgcca cgccaagggt gcagttagac ttgcatccaa caggaagcgt taacggcagc
     1321 aactacgcgc aagatctgac catgttggac atgtgcctgt acgatggttt taacggtaat
     1381 gccatcagtt atgaaatcat gctcaaagat gaagggagac cagctgcagg gcgcagagac
     1441 ggtgacttct ctatctatcg tcagggagga acctctaccg acgagggaga acgcattgat
     1501 taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg caataatgaa
     1561 aatatggtct ggaacagcat taacctgaaa agtgtgcgtc cggtggtact gcccggtatt
     1621 cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa attcagcgtg
     1681 atggataaac aggccgggta ctacatgggc aaattgtcag taatctttac gccttccttg
     1741 ccaaccatca attaaccctg caaagcggac aaacgctttt ttctatataa acgacgaagc
     1801 cgcctgataa ggcggcttcg tcgttataac ttacgttaaa cgttaatgtt gcggtgcggg
     1861 atcgttatgt gattcctgcg ctacctcaat cgccggtttg ttctgcaaca cggtccagat
     1921 aaccagcgca cctaacaggt cgaacactgc cagtactgcg aacagcgggc tgaagccgat
     1981 ggtgtcagcc agtgcaccga caaccagcga aaacagcgta cttgccagcc atgcggacat
     2041 cccggttaaa ccgttcgtcg tcgccacttc gttacgaccg aacacgtcag aagagagcgt
     2101 aatcagcgcg ccagacagtg cctggtgagc aaaaccaccg atacacagca gcataattgc
     2161 gacatacggg ttggtgaaca ggccgatcat acccgggcca atcatcagca ctgcacccag
     2221 cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct ggaacagcgg
     2281 cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca tccaggcgaa
     2341 catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagcg ggatccacgc
     2401 gttaaaagta ccccaggctg gttctgccag gaaacgcggc agcgcgatac cccagaactg
     2461 acggttacgc aggatctgac caacggacat tttcttcgcc gtgctcacct ggtgctgggc
     2521 ttcctgacca ttaataatat agtcgcgttc ttcatcggtc agatgcttct ggtcgcgcgg
     2581 atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaatgcac cggagatgat
     2641 aaatgccatc tgccagctgt gcattacgat tgcccatacc accagcggcg gcgcaatcat
     2701 cgcaccaatc gaagaaccta cgttaaagta acctaccgcg atggaacgct ctttcgccgg
     2761 gaaccattcg gagctggctt tcagacccgc cgggatcatc gctgcttccg cggcaccgac
     2821 tgcaccacga gcaatcgcca ggccacccct gctaccagcc agcgcggttg caccacagaa
     2881 tacggcccac agtacagcaa acattgcata accgattttc gtacccagca catccagtac
     2941 atagcccgct accggttgca tgaccgtata agcagcagaa taggctgcga tgatatagga
     3001 atactgttgg gtggagatgt ttaactcttc catcagagtt ggcgcagctg ccgccacagt
     3061 gttacgcgtc aggtaaccaa gcacggtgcc aagcgtcacc agtgcgatca tataccaacg
     3121 taacccttta attttacgca tgtaaaacct catcgttttg ttatatccgc aggaagcgcg
     3181 gccatcatcc cgccagaggc tgaagatgtt agttacgacc gggtaaccga aaaaaaaccg
     3241 gcacgccccg aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc
     3301 cgattcatct aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga
     3361 cggcagaaag ataacttgtc atacaacttt aaaaggtgag agccatcata aatgtgcgaa
     3421 tatttgtagg gatattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga
     3481 tttaccactt tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc
     3541 ttcgaactct ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct
     3601 cactcgttga gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata
     3661 ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc
     3721 attgccattt gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca
     3781 tctggctgaa aggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggccgagc
     3841 gtctgtgtac cggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc
     3901 cgcacactat cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgtttg
     3961 gtatcactgg caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg
     4021 aactgctggc gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa
     4081 tggtcggcac caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag
     4141 acggctcttt caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaacatcg
     4201 aacaggcgac ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc
     4261 gccgctttgc tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg
     4321 gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg
     4381 aactggacag cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg
     4441 cacagttcaa aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg
     4501 tacagcagta ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg
     4561 ggccggatgt aggctttgac tccatcaacg accgtccgat ggcagaagag ttgtctaagc
     4621 tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac cattctgtac tgcctgaacc
     4681 cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg
     4741 gcaaaatgca gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc
     4801 agatgaccca actggcgcag ctcggtttgc tgagccgctt tgtcggtatg ctgactgaca
     4861 gccgtagctt cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga
     4921 tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg
     4981 tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc
     5041 tggggttgat atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc
     5101 ggatttggct gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga
     5161 tgttgctcgt ggacataaat ttgcgttaac ggatatcgca aaaggggcca atgtcatcaa
     5221 atatggcctg ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc
     5281 ccacaatacg cgcacgaatc tgagcgatct ggatcagtcc tgattttcag gatctgcctg
     5341 cgcaagcggc agatcgtgaa gtgcagatct atcgtcgcgc taacggcgat gtcggggggc
     5401 gtaacgagct gtggatcctg ccaaccgtgg gctgtgtcaa cggcatcgcg cggcagatcc
     5461 agaaccgttt tctgaaagag accaacaacg ctgaaggtac cgacggcgtg ttcctcttca
     5521 gccacaccta cggctgctca cagctgggcg acgatcacat taatacccgc accatgctgc
     5581 aaaacatggt gcgccacccg aacgcaggcg cagtgctggt gattggtctg ggctgtgaaa
     5641 acaaccaggt tgccgcattc cgtgaaacgc tgggcgatat cgatcctgaa cgcgttcatt
     5701 tcatgatctg ccaacagcag gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc
     5761 tgtataacgt gatgcgcaac gataaacgcg agccaggcaa actcagcgaa ctgaagtttg
     5821 gtctggagtg cggtggttct gacggtcttt ctggtattac tgctaacccg atgctggggc
     5881 gtttctctga ctacgtgatt gctaacggcg gtactaccgt actgaccgaa gtgccggaga
     5941 tgtttggcgc agagcagttg ctgatggacc attgccgcga cgaagcaacg tttgaaaaac
     6001 tggtcaccat ggtcaacgac tttaaacagt actttattgc ccatgatcag ccgatctacg
     6061 aaaacccatc accggggaac aaagcgggcg gtatcaccac gctggaagac aaatcacttg
     6121 gctgtaccca gaaagcgggt tccagtgtcg tgggtgacgt gctgcgttac ggcgagcgtc
     6181 tgaaaacgcc agggctgaac ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg
     6241 ccctggcggg tgcgggctgc catatggtgc tgttcagtac tggtcgcggc acgccatacg
     6301 gtggatttgt gccgacggtg aaaatcgcca ccaacagtga actggcggcg aagaaaaaac
     6361 actggctcga ctttgacgcg ggtcagctga tccacggtaa agcgatgccg cagttgctgg
     6421 aagaatttat cgacaccatc gttgagtttg ccaacggtaa acaaacctgc aacgagcgta
     6481 acgacttccg cgaactggcg atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt
     6541 tgttaataaa aaacggcgtt tcataatgtg aagcgccgtt tgcttttcca tacctgataa
     6601 ggatttatca tgaccgcgta ttggctggcc cagggcgtgg gtttcatcgc ctttctgatt
     6661 ggtatcacaa catttttcaa tcgtgacgaa cgtcgcttca aaaagcagct ttcggtctat
     6721 agcgccgtta ttggcgtaca tttttttctt ctgggcacct atcccgctgg tgccagtgcc
     6781 atccttaatg ccattcgtac attgattacc ttacgcacgc gcagcttatg ggtaatggcg
     6841 atttttattg tgctgactgg cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta
     6901 ttgccggtta tcggtacgat tgtcagtacc agggcgctgt tccgctgtaa agggctcacc
     6961 atgcgctgcg tgatgtggtt ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg
     7021 tcgataggcg gcacaatgat tgagggtagt tttctgctta tgaatggctt gaatatcatt
     7081 cgtttctggc ggatgcagaa aaggggaatt gatccgttta aagtagagaa aacccctccc
     7141 gccgtagacg aaaggggtta aacaattaat tacgcagggc gctatttgcc agacgatcgt
     7201 cttccgcctg gcaagctgcc gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt
     7261 cgcaagagtc ctgcaatacg ccgatgataa agccgacggc aaccacctgc atggcgatat
     7321 cgttcgaaat accgaacata ttacaggcca gcgggatcag cagcagagaa ccccccgcca
     7381 cgccggatgc gccacaggca cacagagaag ccaccacgct caacagcagc gccgtgggca
     7441 gatcgaccgg gatacccagc gtattaaccg cagccagcgt caacacggta atggtgattg
     7501 ctgcgcccgc catattgatg gtggcaccca gcggaataga aacggaatag gtatcgcgat
     7561 ccagattcag cttttcacac agtgccatat tcaccggaat gttcgccgca gagctgcggg
     7621 tgaagaaggc atacacgccg ctttcgcgca ggcacagcag caccagcggg aacgggttac
     7681 gacgaatttt ccaccacacc agcaatgggt taaccaccag cgccaccagt aacatacagc
     7741 caaccagcac gaccagcagt tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca
     7801 gggtagaaga aaccagacca aaaataccaa tcggtgcgaa gcgaatgacc agtttcacca
     7861 taaaggtgac ggcattcgac agatcgttga ccaggttttt ggtggtctcg ttaccgtgac
     7921 gcagtgcgaa accgaggcca attgcccaca ccaggatccc gatgtagtta cctttcagca
     7981 gcgcgtcgat ggggttggaa accatgctca ttaccagccc gcgcatcact tctacaatgc
     8041 ctgacggtgg cgaaatatca cccgcgctac tggataagtg cagggtagaa gggaaggcga
     8101 agctgaaaac gactgcggcc agagcagcag agaaagtacc cagcagatag aggaacaaaa
     8161 tagggcggat attggttttc tgcccgtgct ggtggttagc aatagatgcc atcaccagca
     8221 tcaacaccag gatgggggca acggctttca gtgcgccgac gaacaaagta cctaacagac
     8281 caacagcttc cgccgcgggt tttgagatcc atgccagaag aatccccaga acaaggccga
     8341 ccaggatttg ttttaccagg ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt
     8401 gcgtagtcat ttttcgatcc tttcattgtg ttgtcgcaca tccctggtgc gttctgtcga
     8461 cgcatctttc aggatgtaac aaagtgtttg caaggtcgag tataaggaaa gttacagctt
     8521 cgggaagcga aaaatgctgg attttaaaga gaggtcatat tttgcaactt aattgttata
     8581 tttgtttgac ataaacagag ggaaggactg gctactaagg ctgattagat aacatatggg
     8641 caaaactatt tggaatatga gaacggttgc gctcctttaa tgtgctatgc catataaagt
     8701 cactggcgca gggagcgcgc agggggggcc aatcgccgcc gccccctgca cccccaggct
     8761 ctggcgaaca aaatcgccgc tgcgcgattc cctcagctta tcccttgcgg ctaccgggtc
     8821 gggcgcaagg gaacgtccct gtaagatgcg ccctcgccgc gcatccatgc gcgtcgcccc
     8881 ggcctccagg gaacgcttcg gcgattttga cgcaaccaac acccgtgcgg tctttttgta
     8941 aagagctaca agccaattac ataattaata gctcaccttt gtctggaatg agttatctct
     9001 aggaatggct taatatacag cgaatcattc tccccgctgc ttatcatgcc gataattcac
     9061 ccaggcgttg ataataaacg tcatcaccag aatgccaaac accacgccca gcgagacggc
     9121 gattggaata tggtagaagt cgacaatcag catcttgata ccgataaaca ccaggatcac
     9181 cgccaggcca tatttgagca tcgagaaacg ctctgctacg cccgccagca ggaaatacat
     9241 cgcacgcagg ccgaggatcg caaacaggtt tgaggtcagc acaatgaacg ggtcagtggt
     9301 cacggcgaag atagccggaa tgctatccac ggcgaaaatc acgtcgctca attccaccag
     9361 aatcagtacc agcatcagcg gtgtggcgta cagcaatcca ttcttacgca caaagaaatg
     9421 ctcgttgtcg atggtgtcgg tcatgcgcaa atgaccgcgt agccagcgca ccagcggctt
     9481 gtcgccaatg cctgattcat cttcatgggc gagcgccatc ttcacgccgg taaacagcag
     9541 gaaggcaccg aagatataca ggatccagtc gaactgcgaa attagccagc tgccagtgaa
     9601 gatcatgatg gtacgcagaa caatcgcccc gagcacgcca tacaccagca cgcggcgttg
     9661 taatgccgcc ggaacagaga aatagctgaa caacatcagc cagacaaaga cgttatcgac
     9721 cgccagcgat ttctcaatca gataacccgt gagaaaggcc agtgcctgtg gttcggcaac
     9781 ggcgcgacct tcggtttgca ccagatacca ccagaaagcg gcgttaaaca gtaacgacag
     9841 cgtcacccaa accagcgacc aggccgcagc ctgtttcatg gtcatggcat gtgccccacg
     9901 acgcccctgc aacaacaggt cgatagccag cataatggcg acaacaacag cgaatccgcc
     9961 ccatagcaac ggcgtgccga cagtattcat agaagttcct tacacataaa aaaaacgggc
    10021 aacgtcggaa gacgtcagcc gctgcttttt atgcatagac ctcgccttcc ggcaaggtct
    10081 cacttacaac aaaaaaggaa tacgtctgcg tattcctttc gggttgcccg gtgaccggat
    10141 gtggtttttc acacatcgta atgacgatcg accggcaatg aagttactcc cctttgcagg
    10201 taacaaagta tgacagacca ttcagccagt caatggtctg ttacatacct tttacactgt
    10261 tttactttgg caattatagt tttacgctat ctgccggaaa aatcacccca gtctggcggc
    10321 ggatctcagt cagcagtttg gcggtgatgc gactgaccgc cagccctgga tgatccacca
    10381 gatgctcatc caccagggta gcaaacagct ctgcttcgta gagcatggta ttaatatgct
    10441 gcggctgggt gagatcctgc atctggctgc cacgcggcac gaagcacact ttctggcatt
    10501 cagacagttt ttcaatcacc agcgatcccg cttcgccctg aatctcgctt tccaggacag
    10561 aatcactgac ctttgagtgc agcaacgtaa cgctgaaatc accgtaatcc atcaccacca
    10621 cgccgtgggc atctacgccg cttgccaaca aactggcggt tgcctgcacg cttttcggtt
    10681 caccgaataa tgccaccgcc gacgccaggc agtaaaagcc gatatccata attgaaccgt
    10741 tagagaatgc cggattaaag gtgttgggat tctcaccatc caggtaacgt tgataccgcg
    10801 aggaatactg gcaatagttg aaaaagactt tacgcaattt gccgactttc ggcagcgcct
    10861 ggcgcagcaa atgaaagttc ggcaggctgg cggttttaaa tgcctcaaac agcaccacct
    10921 gattttcccg cgcacaggca atggcggcat ccacttccgc cagattcgac gccagtggtt
    10981 tctcgcaaat cacatgaatt ttatggctaa ggaaaagttg tgtctgggaa aagtgcaggg
    11041 cattcgggct ggcaatatac accgcgtcaa tggcatcgct ttccgccatc gcttccagcg
    11101 aggtaaacag atgctcgaca gaaaaatcat tggcgaagtg ctgggcctgt tcaaggctgc
    11161 gggaatatac ggcggttaac ttgtatttac cgctctcatg ggcggcctcg acgaactggc
    11221 gagtgatcca gttcgtacca atcacagcga aacgtatcat aacgcgtgga tactccataa
    11281 agggattctg tcgtcaatgt agcacgcctt tttcatcttc gacgtgcgca gatacgcatt
    11341 attcttttgc cggacagcca tgctcactaa acggcccaat catgaccgat ggcccgagat
    11401 atttcggctg gcgatggagg attttggcgt caatcacggc gctgacacga gcgagtttct
    11461 cccgcagttg agtataaaaa ccgtgcttca tattgaggtc aggcgcgtta aaaccgatag
    11521 cgtcgatgct gtactgtttt gccagccaga tggcgcgttg gttatgaaat tcttgcgaga
    11581 taatggtgat atggttttca ccaaaaacct ttttggcacg cactaccgaa tccagcgttg
    11641 agaatccggc atagtcgcag aagatcactt tcgccggtac gcctttagcg atcaaagcct
    11701 tctgcatacc tgattcttca tcgtaatttt tacgtccgtt atcgccactt accagtagcc
    11761 atttcacttt tccggcgtgg tacaacgctg ctgcggtatc aatacgtcgg gtaaagtagc
    11821 gattacccgg cctggccccc agcaataagc ccgcgttacg cgccggaact gcgttgacat
    11881 cgctccaggt cagctgcttg ctggcgttga ccattactcg atcggcgata aaaatggtcg
    11941 ctcctgccac aagtaacagt aagcaggcaa tctttagagt ccggcgagaa aaacaaatac
    12001 ggagaagaag gcggggctgc atggtcgtta tccttaacga aaggggggag gtcgatttta
    12061 ggcgctaaag aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc
    12121 gacaactgcg ttagccacag ccgggtgtcg aactccagct ggtggtactg cggctccata
    12181 tggcagcaca actggtaaaa cgctttgtta tgctctttct ccttcaggtg cgccagttcg
    12241 tgcaccacga tcatacgtaa aaacggttcc ggcgcgttgc gaaacacagt agcgacgcgg
    12301 atctctgctt ttgctttcag cttgccgccc agcacacgag aaacagcggt atgtaacccg
    12361 agcgcgtttt ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca
    12421 ttgcgcagaa actgattttt tagatcctga gtatattgcc agagggcttt atcggtggcg
    12481 tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc
    12541 agcgttcgca cctgggaaag tagctgctcg ggataaccct gaagataagt aagattgctc
    12601 ataaccgccc acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt
    12661 cgaaatttta tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga
    12721 cactacaacg ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcgg
    12781 atgaatattt gctgcaacag ttggacgaca cagaaatccg cggcccggtg ttgatcctga
    12841 atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg
    12901 actcatacat cagtgaactg gcgacgcgcg agaatttacg cctcaacggg attgatgagt
    12961 cgagcgtgaa gtttctcgac agcaccgccg actacccgca agagccgggt gtggtgctga
    13021 tcaaagtgcc gaaaacactg gcattgctgg aacaacaact gcgtgcgctg cgcaaagtgg
    13081 tcacgtcgga tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc
    13141 tggaactgtt cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc
    13201 gcctgattaa ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct
    13261 ggaagctgga aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg
    13321 ggcttgatat cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga
    13381 ttgtcgatct cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc
    13441 aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg
    13501 ttgaaaccaa catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct
    13561 ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa cccgccgttt caccaacaac
    13621 atgcgctgac cgataacgtc gcctgggaga tgttccacca tgcccgccgc tgcctgaaaa
    13681 tcaacggcga gctgtatatt gttgccaacc gtcacctgga ttacttccat aaactgaaga
    13741 agattttcgg caactgcacc accatcgcca cgaataataa atttgtggtg ctgaaagcag
    13801 tgaagctggg gcgtcgtcgg taagattggg agttacctgc cggatgcggc gttaacgcct
    13861 tatccggcct acgttcagca cgttactccc aggccggata agacgcggca agcgtcgcat
    13921 ccggcaatgt ctgcccagtt ctggaatcgc gcttccataa aacaaaaaat ccctctcacc
    13981 atcctctaca ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa
    14041 ttcatccgtt gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg
    14101 atgaagaatg cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca
    14161 acataaaacg gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc
    14221 tgagtcatta aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta
    14281 tgttttcaat gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca
    14341 aaagtgctac atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca
    14401 tcgtactaag gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta
    14461 actgcggtgg caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg
    14521 gaactggtag atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt
    14581 gccaaaataa ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct
    14641 caagccatgc ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc
    14701 cttgccgatc tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag
    14761 aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc
    14821 ttgtttattg attaacgcga cgtgccggat gcgttgcatc cggcgcttca aataacgatt
    14881 taaatctcca gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata
    14941 gctacatcgc agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc
    15001 agcgcgcggt ttggctcttg ccctgcgcag atcaccacat tgtccacagc taatacctgc
    15061 gtttcgccgt tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct
    15121 gggatcattt tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg
    15181 cctaacccct gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta
    15241 cgcgggatct gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc
    15301 ccccactcat tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa
    15361 tacatcgccg tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc
    15421 ggcgctttgt cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg
    15481 ataggcggaa cgcgcggcac gatcctactg gcgaggatcg tttcatcgaa cgcctgtaac
    15541 tgatccgccg tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg
    15601 tggtaatagc gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta
    15661 aactgcccgc cgatctcgct gtgggcatca aacaatgtta cctgatgccc acgcgccgcc
    15721 gcgttaatgg caaacgccag cccagcaggt cccgcaccga ccaccgccag attttttttc
    15781 tgcacggcag gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac
    15841 gaggtgactt tgccaacgaa gatctgatcg aggcaggcct gattgcagcc aatgcaggtg
    15901 ttgatctcat cggctcgccc cgattgcgct tttgacagca gttccgcatc agcaagaaac
    15961 ggtcgtgcca tcgataccat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc
    16021 ggatcgttaa tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc
    16081 gtgacccagc taaatgcgcc gcgcggcacg ggcgtggcaa tggtcggaat acgtgcttca
    16141 tgccagccaa tgccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagc
    16201 tctaccgttt cggcaaaagt cccgccgtct tcgaccaggt cgagcatcga cagtcggtag
    16261 ataataatga agtcgttgcc gacgcgttcg cgcaccgcac gcactacttc tacggcaaat
    16321 cgcatccggt tgcggtaatc gccgccccac tggtcactac gctgattggt gtgcagcgtc
    16381 agaaattcgt tgatcaaata cccttcggac cccattacct ctacaccgtc gtatcctgcc
    16441 tcccgtgcca gttgcgcgca gtgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg
    16501 gtgagttcgt gaggaacgaa acggttgatg ggggcctgca atgcggaggg agcgaccaga
    16561 tgcggttggt agctgtagcg cccggtatgc aaaatttgca gggctatttt gccgccttcc
    16621 tgatgtaccg cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt
    16681 gcgccacctt ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc
    16741 gccacgccgt gacgggcgcg ttcggcataa aacgctgcca gccgctcggc accgtccggg
    16801 tattcctcca gcccggtgtg cattgagccc atcaacacgc ggttttttaa cgtggtaaaa
    16861 cctaaatcca gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat
    16921 aattgttatg tggtcggatg agttctaatt tagccgtcac cggagaaaag ggaaaagggg
    16981 agtgcggggt ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac
    17041 gcttgccgtg ttttatccgg cctgggggat tgcacatgta gggcggataa ggcgtttacg
    17101 ccgcatccgc cagcagttgc aaattattgc ttacggaaaa aatcgttata caacatatac
    17161 aaatgcgcgg cactccagga gaaatttggt gcgccttgct gcgcgccagt cagcgggttg
    17221 taattctcct gaattgggcc atcggcggtt aatcctttgg cgtgccggaa gaacgtatcc
    17281 gccagcttca gggcatcatc acgataaccg taacgctcca tccctttcag agcaaaccag
    17341 aactgatcca cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt
    17401 aacgccgccg ttcccagcgg gacaaaggtg ttgaactctt taggatcgag catcaccttc
    17461 accaccgcgt cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc
    17521 ggccctttac cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct
    17581 tcaatacgta cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag
    17641 tccgcgagct gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg
    17701 gttgccatct ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac
    17761 tcctgcaata gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc
    17821 accgtccagt cgctacgttt gccaccatta gcgacatatt tatccaactg ttctttgtcg
    17881 ataaaaccaa agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc
    17941 ggaatttcca gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca
    18001 gactgcgtct cttctttgtt gcctttcttc accgcaaaca gcatctcgcc gctctcagtg
    18061 ttgtgggctt tgtcgcgggt cgcgccatat tccggcacgc cgttgccgtt gtgatcgcgg
    18121 ttacgtaacc accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct
    18181 ttatcctggg tgacgttgta cacttctatc accgaccagg cggcaaggct gggtttggta
    18241 ttacgttcgt tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc
    18301 aggtcgggga caaagcccac atcctgcgga cgcacgcggt cgccaggctg gatctgccag
    18361 gagaagaccg cgcggatatt ctctttggcg atgtccggat tgaaatgcgc catcgcaaac
    18421 gcctgcttct aggtatccca cggccaggtc tgattgccgg agaaccagcg cccggtcacc
    18481 gacggtgtaa cggtgttaaa tttcaccgca ccgcccggtg agcgccagtt accgttgagc
    18541 gtttcgatcg ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac
    18601 cctttcttca ggtattcttc ccagcgttgc tgcgagtcgg tgagataaaa cgccggacgc
    18661 gccagaatat cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg
    18721 gaataggtgg tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta
    18781 ccgttgattt cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag
    18841 gtcagcagat cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg
    18901 gcgctgattt tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt
    18961 ttcccttctt tcgcttccag tttttccagc agttcgccgt cccacaccag atccagcggt
    19021 ttattgctgg tgattttggt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc
    19081 atttcgactt gcacatcttt tgctgtcagt ttttgcacca gcgcaccggg aatactgtat
    19141 gcctccagcg taaagtcgac tttcttgccg tcctgccaga cggtcaggcg gtcgaaattg
    19201 ctggccataa agttgatgta ctcttccgtc agcagcgcaa cgcccggaaa gccgcccatg
    19261 gtgttagggc cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta
    19321 aaacgctggt gatcgtcgta atcgtaatcc ttcatgtact gcggcgcgcc agtacggtta
    19381 atcacgtttt tataattgtc ggcgttagcg gcatgggcgg aaaaacttat cagcagagcg
    19441 caggttactg gcgttaaaat agtttttatt ttcatgaaat tacagtcctt attaccagta
    19501 gaaatattgc atcaggaaga tttggtcgct gggatcgtga gaggcatttt tcatgtagaa
    19561 gcggctatca aaagccgtgg cgaaacgtcc gccgccgtat tcgtaccaga taccaaactg
    19621 catgaaagag gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc
    19681 ggtggagaga taaacgccct ttaacgcttc atacatggcg ctcaccatca tcccactttc
    19741 acttcccggt acgtccagcc cgtcggcgcg tccggtatgg tgccaggcgc gagcggcaaa
    19801 gttaggagtt agcaggcccg tgagatagag ctgtcctgtg ccgtcggtga tttccagacc
    19861 gttcatccag gtgatgcctt cggcaaggtc atactggatg taaccgttca tcattgccgg
    19921 ataggtgtat ttgtcgtcat agcgatcgta tttgccaaaa tgtagccagg ctttgctttc
    19981 atcatgatgt ccggcaggag tcgcggtgac gctataacga aagttgccgc ttaagttctg
    20041 taccttcagc gcagtcataa tgtcgcgggt attgggaatg acgtagccca gatcgggggt
    20101 gaaatcgccc caccattgca ggtcatccag cgaggaatcc tgacgcagac tgagcatgat
    20161 ttcagtgcca tcattggtac gataaccgcc atagaaacgg ttaattccgc cctcgaaacc
    20221 accccagcta tggtctggca cccaggcatt gccgtcgtgg tcggcctgca cggtccagcc
    20281 ttcgccgtaa atcaggccga accaggagcc gtacttcatc tccaggccac cttcaatata
    20341 ggtaccgtcg ctgtatttat gtttattctc accc
//
LOCUS       DAJTNA010000064        20187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTNA010000064 DAJTNA010000000
VERSION     DAJTNA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20187
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.065"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..121)
                     /locus_tag="OO161_001943"
     CDS             complement(<1..121)
                     /locus_tag="OO161_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose specific IIB subunit"
                     /protein_id="HCR5463018.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIP"
     gene            complement(149..592)
                     /gene="cmtB"
                     /locus_tag="OO161_001944"
     CDS             complement(149..592)
                     /gene="cmtB"
                     /locus_tag="OO161_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR5463019.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIGFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(906..2897)
                     /gene="tkt"
                     /locus_tag="OO161_001945"
     CDS             complement(906..2897)
                     /gene="tkt"
                     /locus_tag="OO161_001945"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5463020.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKDAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            3175..3933
                     /gene="loiP"
                     /locus_tag="OO161_001946"
                     /gene_synonym="yggG"
     CDS             3175..3933
                     /gene="loiP"
                     /locus_tag="OO161_001946"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5463021.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4139..5059)
                     /gene="speB"
                     /locus_tag="OO161_001947"
     CDS             complement(4139..5059)
                     /gene="speB"
                     /locus_tag="OO161_001947"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5463022.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5196..7172)
                     /gene="speA"
                     /locus_tag="OO161_001948"
     CDS             complement(5196..7172)
                     /gene="speA"
                     /locus_tag="OO161_001948"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5463023.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVSTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7181..7312)
                     /gene="yqgB"
                     /locus_tag="OO161_001949"
     CDS             complement(7181..7312)
                     /gene="yqgB"
                     /locus_tag="OO161_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5463024.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            7448..7663
                     /locus_tag="OO161_001950"
     CDS             7448..7663
                     /locus_tag="OO161_001950"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463025.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            7967..9121
                     /gene="metK"
                     /locus_tag="OO161_001951"
     CDS             7967..9121
                     /gene="metK"
                     /locus_tag="OO161_001951"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5463026.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            9557..10951
                     /gene="galP"
                     /locus_tag="OO161_001952"
     CDS             9557..10951
                     /gene="galP"
                     /locus_tag="OO161_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5463027.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRYTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11028..11525
                     /locus_tag="OO161_001953"
     CDS             11028..11525
                     /locus_tag="OO161_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5463028.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVVK"
     gene            11620..12327
                     /gene="endA"
                     /locus_tag="OO161_001954"
     CDS             11620..12327
                     /gene="endA"
                     /locus_tag="OO161_001954"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5463029.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            12407..13138
                     /gene="rsmE"
                     /locus_tag="OO161_001955"
     CDS             12407..13138
                     /gene="rsmE"
                     /locus_tag="OO161_001955"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5463030.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13151..14101
                     /gene="gshB"
                     /locus_tag="OO161_001956"
     CDS             13151..14101
                     /gene="gshB"
                     /locus_tag="OO161_001956"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5463031.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTCTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIAHQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            14210..14773
                     /locus_tag="OO161_001957"
     CDS             14210..14773
                     /locus_tag="OO161_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5463032.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPERRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            14773..15189
                     /gene="ruvX"
                     /locus_tag="OO161_001958"
     CDS             14773..15189
                     /gene="ruvX"
                     /locus_tag="OO161_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5463033.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(15365..16345)
                     /locus_tag="OO161_001959"
     CDS             complement(15365..16345)
                     /locus_tag="OO161_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5463034.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            16363..17067
                     /gene="yggS"
                     /locus_tag="OO161_001960"
     CDS             16363..17067
                     /gene="yggS"
                     /locus_tag="OO161_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5463035.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17085..17651
                     /gene="yggT"
                     /locus_tag="OO161_001961"
     CDS             17085..17651
                     /gene="yggT"
                     /locus_tag="OO161_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5463036.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGLWMAL"
     gene            17648..17938
                     /locus_tag="OO161_001962"
     CDS             17648..17938
                     /locus_tag="OO161_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5463037.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            17946..18539
                     /locus_tag="OO161_001963"
     CDS             17946..18539
                     /locus_tag="OO161_001963"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5463038.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            18532..19668
                     /gene="hemW"
                     /locus_tag="OO161_001964"
     CDS             18532..19668
                     /gene="hemW"
                     /locus_tag="OO161_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5463039.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGRKVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(19823..>20187)
                     /locus_tag="OO161_001965"
     CDS             complement(19823..>20187)
                     /locus_tag="OO161_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5463040.1"
                     /translation="FNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDF
                     DINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQA
                     NERWKKDTEQLRRNGVIK"
BASE COUNT         4864 a         5290 c         5194 g         4839 t
ORIGIN      
        1 tgggaataaa taacgcagta ataaaacccc aggcaataaa agcaccaata ttggggatga
       61 ccattgcagt caaaaagccc ccaaaagcct ggacctttgc acgagcagac ttgttttcca
      121 taatattatc ctgtagagga gagagtaatt atccgcggct gataatgtcc gctaattgtt
      181 tttctgatga tgctgttaag agttgttcga gtatttcttc ttcgcacagt aattcactta
      241 acgcctgaat agcgccaatg tgcgaatcgg catcggcagc agagagtccg atgagtaatt
      301 taattggttc gtcattcccc ggaaaataaa caccttgttc aagtaatgtc aaagacatcc
      361 cggttttaag cgccccacat tccggtcgcg catgaggcat tgccacgcct ggtgcgagaa
      421 tataataagg gccattgtta atggtggaat ctttaattgc ctgaatgtaa ttctcgctga
      481 tatagttttt atccagcaat gataccatcg agaaaccgat agcttcctgc caatcttttg
      541 ctgaatgtat aactgagatt gatgattctg gaaaatagtc actaagccgc ataaattatc
      601 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      661 aaagcaattg agcagggaaa ccggtgtttt gaaaacagtg aattattaat ttcttatata
      721 acattgagtt ataggtataa caacaaatgt caatacgcat atcgtgatgc atattatata
      781 attgatattt attgcatata aatatttgtg atctgcaaca cgcttcatct attgcttgtc
      841 cgcaaacgga catatcaagg taataaaaaa ggtcgccgaa gcgacctttt tacccgaaat
      901 gctaattaca gcagttcttt tgctttcgca acgacgttgt cgacggtgaa gccgaactct
      961 tcgaacagca actctgccgg agctgactca ccgaaggtgg tcataccgat gatagcgcca
     1021 ttcaggccaa catacttgta ccagtagtca gcaatacccg cttctaccgc aacgcgtgca
     1081 gtaaccgctt tcggcagtac ggattcacgg taagcagcat cctgcttgtc aaatgcgtcg
     1141 gtagacggca tggacaccac gcgcgctttc acgccttcgg cagtcagttt ttcgtaggca
     1201 gcaacagcca gttcaacttc tgaaccggta gcgatgaaaa tcagttccgg ctgaccggcg
     1261 cagtctttca gcacataacc accgcgcgcg atgtttgcca gttgctcttc agttcgttcc
     1321 tgctgcgcca ggttctgacg ggagaggatc agtgcggtcg ggccgtcctg acgctcaaca
     1381 ccgtatttcc acgcgactgc ggattcaacc tggtcacacg gacgccatgt agacatgttc
     1441 ggggttacgc gcagagaagc gacctgctct accggctggt gagtcgggcc atcttcgccc
     1501 agaccgatgg agtcgtgggt gtaaaccatc acctgacgct gtttcatcag cgcagccata
     1561 cgtacggcgt tacgtgcgta ttccacgaac atcaggaagg tggaggtgta cggcaggaaa
     1621 ccaccgtgca gggagatacc gttagcaatc gcggtcatac cgaactcgcg aacaccgtag
     1681 tggatgtagt tacccgcagc atcttcgttg attgctttag aaccagacca cagagtcagg
     1741 ttagacggtg ccaggtcagc agaaccgccg aggaattccg gcaacagcgg accgaaagct
     1801 tcgatagcat tctgagacgc tttacggctg gcgattttcg ccgggttagc ctgcagttta
     1861 gcaatgaact cttttgcttt cgcgtcgaag tcagacggca tttcgccttt catacggcgg
     1921 gtaaattcag cggcttcctg cggataagct ttcgcgtaag cagcgaattt ctcgttccat
     1981 gcggattctt tcgcctggcc tgcatctttc gcatcccact gagcatagat ttcagacggg
     2041 atttcgaacg gtgcatattt ccagcccagc tgttcgcggg tcagggcaat ttcagcgtcg
     2101 cccagcggcg caccgtggga gtcgtgagta ccggctttgt tcggggaacc gaaaccgatg
     2161 atggttttgc acatcagcag ggacggtttg tcagtcactg cgcgcgcttc ttctactgcg
     2221 cgtttgatgg atgccgcgtc atgaccgtcg atgtcgcgaa taacgtgcca gccgtaagct
     2281 tcgaaacgca ttgcggtgtc gtcggtgaac cagccttcaa cgtgaccatc aatagagata
     2341 ccgttgtcat cgtagaacgc aatcagttta cccagcttca gcgtacccgc cagagagcaa
     2401 acttcgtggg agatgccttc catcatgcag ccgtcgccca tgaaggcgta ggtgtagtgg
     2461 tcgacaatgt cgtggcccgg acggttaaac tgcgccgcca gcgttttttc tgcaatcgcc
     2521 ataccgactg cgttggcaat accctgaccc agcggaccgg tggtggtttc cacaccagcg
     2581 gtgtaaccca cttccgggtg gcccggagtt ttagagtgca gctgacggaa gtttttcagt
     2641 tcttccatcg gcagatcgta accggtgagg tgcagcaggc tgtagatcag catggagccg
     2701 tggccgttgg acagcacgaa gcggtcacgg tcagcccagg acggattctg cgggttgtgt
     2761 ttcaggaaat cacgccacag gacttcggca atgtcagcca tacccatagg ggcacccggg
     2821 tgaccggatt tggctttctg tactgcgtcc atgctcagcg cacgaatagc attggcaagc
     2881 tctttacgtg aggacatttt gactccagat cggatgatga agggcacgcc cttaacgact
     2941 tgacgacagc gcgttttggg ctacgccgga aaatttgcca acaatttacc gcaagccgcg
     3001 cgtcatgtac atggaacatc cttttgccgc ttcagaaatc tctggatcat gctcgcatgt
     3061 tgcgcaatct actcgcccgt ccgctgcgct tttccttata ctgagactga gcgtcgattc
     3121 acctgcaaac ggcgcatttt tagaataatc ctgaccttgt gcggaagaga aaacatgaaa
     3181 attcgcgcct tattggtagc aatgagcgtg gcaacggtac tgactggttg ccagaatatg
     3241 gactccaacg gactgctctc atcaggagcg gaagcttttc aggcttacag tttgagtgat
     3301 gcgcaggtga aaaccctgag cgatcaggca tgtcaggaga tggacagcaa ggcgacgatt
     3361 gcgccagcca atagcgaata cgctaaacgt ctgacaacta ttgccaatgc gctaggcaac
     3421 aatatcaacg gtcagccggt gaattacaaa gtgtatatgg cgaaggatgt gaacgccttt
     3481 gcaatggcta acggctgtat ccgcgtctat agcgggctga tggatatgat gacggataac
     3541 gaagtcgaag cggtgatcgg tcacgagatg gggcacgtgg cgttaggcca tgtgaagaaa
     3601 gggatgcagg tggcgcttgg tacaaatgcc gtgcgagtag ctgcggcctc tgcgggcggg
     3661 attgtcggca gtttatctca atcacaactt ggtgatctgg gcgagaaatt agtcaattcg
     3721 caattctccc agcgccagga agcagaagcc gatgattatt cttacgatct tctgcgccaa
     3781 cgcggcatca gcccggcagg tcttgccacc agctttgaaa aactggcgaa actggaagaa
     3841 ggccgccaaa gctcaatgtt tgacgaccat ccggcatccg ccgaacgcgc ccagcatatt
     3901 cgcgatcgca tgagcgcgga tgggattaag taaagcctgg tggtgtcgaa cgtaggtcag
     3961 ataaggcgtt cacgccgcat ctgacattaa tggcacattt gttaccttgt gcgcattgcc
     4021 ggatgcgatg ctggcgcatc ttatccggcc tacgggtgcc gaacgtaggt cggataaggc
     4081 gttcacgccg catccgacat taatggcacg ttttacccgt gcgcatcgca tctggtgctt
     4141 actcgccctt tttcgccgcc tgaatataca gcatctccag cgccagcgtc gccgcagcca
     4201 gagcggtgat ttccgactga tcgtatgccg gagccacttc cactacgtcc atcccaacaa
     4261 tgttgagatc tttcaggccg cgtaccagtt taatggcgcg atcggaggtc aggccgccaa
     4321 tcactggcgt accggttccc ggcgcgaaag caggatccag gcagtcgata tcaaaggtca
     4381 ggtaaaccgg catatcaccc acaatctgtt tcacttgggc gataacgtca tccacgctgc
     4441 gatcgttcac ctggcaggcg tccagcacgg taaaaccgtt gtctttatca aactcggtac
     4501 gaataccaat ctgcacggaa tgattcgggt cgatcagacc ttctttcggc gcggtataga
     4561 acatggtgcc gtggtcaaat tcacaaccgt tcgcgtaggt atcggtgtgg gcgtcaaagt
     4621 gtaccagcgc cattttgccg aaatgcttcg catgagcacg cagcagcggc agcgtaacaa
     4681 agtggtcacc accgaaagag agcatacgct taccggcagc cagcagcttc tcggcgtgtg
     4741 cctgcagttt ttcgctcatc tcacgggcat cgccaaaggc atataccaga tcgccgcagt
     4801 ccacgacgtt caggcgctca cgcatgtcga aattccacgg gaagcgattg tgttcccagg
     4861 caagattcgt cgaaacctga cggatcgctg ccggaccgtg gcgtcccccc gcacgaccag
     4921 aagtggccat atcgaacggc acaccagtaa tcacccagtc agcatcgctg tcatacggct
     4981 ggaagttcat cggcaggcgt aaaaaaccaa aggcgttgga aaccagtgag ttatcgtatt
     5041 gatgacctaa ggtgctcatg gatggactcc tattacaaag tcgatataaa aaaacccttc
     5101 cgcgtcgtta ggcccgacga ggaagggttg gatttgtcac aataaattgt ggcggattat
     5161 caccgctaaa ttaagcggat tcaagtaata cggacttact catcttccag ataagtataa
     5221 ccgtacaaac ctgcctcgaa ctcttcaagg aactgttgtt gcagttcagc atcaagatcg
     5281 gttttcttca cttgatcgcg gaactgagtt aacagcgttt tcggatcgag ctgcacatat
     5341 tgcagcatat ccgcaacggt atcgccttcg tcagacagtt ccacttctac actaccgtca
     5401 gggaagacga acacgtcaac cgcttcggta tcaccgaaca ggttgtgcat gttgccgagg
     5461 atctcctgat atgcgccgac cataaagaaa ccgagcatcg gcggattctc tggatcgtac
     5521 tccggcattg gcattgtcgt ggcaatacca tcaccatcaa tatagtggtc gatagcaccg
     5581 tcagagtcac aggtaatatc cagcagcaca gcgcgacgtt ccggcacttg atccagccct
     5641 tccagcggca gaaccgggaa caactggtcg atcccccatg cgtccggcat tgactggaac
     5701 agcgagaagt tgacgtacat tttgtccgcc atacgttcct gcagttcgtc gattatcgga
     5761 cgatgcgcac ggttttgcgg atccagttgc ttctgcactt catggcacat gctcagatag
     5821 agttgttctg cccatgcacg ttcttgcagg ctaaaggtgc cggaagagta gccgatatga
     5881 atgtcgtgca gatccatctg actgtcgtgt aaccattcac gcagagaacg gcgagttccc
     5941 ggttcgtgca tctcctgcca ggtttcccac atgctttgca gcgcgcgcgg cgcatcttct
     6001 gcaggcgcgg tcgacaccgt gtattcgtta cgttccacgc cgatgatatt agacaccagc
     6061 acggtgtgat gcgcagtcac cgcacgaccc gattcggtga ttaccgtcgg atgcggcaga
     6121 ccgttttctt cacacgcatc accaatcgcc cagatgatgt tgttggcata ttcattgagg
     6181 ccgtagttta cggagcagtc agactgcgaa cgggtgcctt catagtccac gcccagaccg
     6241 ccgccgacgt cgaagcactg aatattgacg cccagcttgt gcagttccac atagaaacgc
     6301 gcggattcac gaacgcccgt ggcgatatcg cgaatattcg ccatctgcga accgaggtgg
     6361 aagtgcagta gttgcaggct gtcgagacgc ccagcttcac gcagcgtttc aaccagttgc
     6421 agtacctgag tcgcagccag gccgaacttc gatttttccc cgccggaaga ctgccattta
     6481 cccgagccct gcgaagccag acgtgcacgc acgcccagac gaggaacgac attcagacgt
     6541 tctgcttcat ccagcacaat ggcgatttct gacatcttct caatgaccag atagaccttg
     6601 tgccccatct tctcgccaat taatgccagg cggatatatt cgcggtcttt ataaccgttg
     6661 cagacgatga cgctacgggt catgccagca tgtgccagca ctgccatcaa ctcggctttg
     6721 gaaccggctt ccagacccag cggttcgccc gaatgaatca gggactcaat cacgcggcgg
     6781 tgctggttaa ctttgatcgg ataaacaagg aagtaatcgc cgttatagcc gtaggattcc
     6841 ctcgcacgtt tgaacgcggc gttaatggaa cgcaaacggt gctgcaggat ctgtgggaaa
     6901 cagaacagtg caggcagacg ctgaccctgt gcttcacgag ttttcactaa ctgcgcgaga
     6961 tcgacgcgag cttccgggac gtccgggtcc gggcacacgc tgatgtggcc cagctcgtta
     7021 acgtcatagt agttattgcc ccaccaggca atattgtaag tacgcagcat cttgctggct
     7081 tcctgggagc tcattgcaac ctcctgcatg gagcgtagta caccgtgttc gcccgctgac
     7141 gaaggcaaac ccatagacat gtcgtcagac atagcgaacc tcaaattatt ttattaagtg
     7201 taaaacagtt aacgactatc gcagcctgga actgcgataa caacccataa gcacatggat
     7261 tttccagcag tgaatgctga cgctccaact gcgcaaccgg tttctttttc ataacattat
     7321 taagcacata accgaacgta agtgtgacag ttcggcgaaa ccacgagaaa actcttgttt
     7381 ttacaagagc gcccttgttc agtcctcagt aactgtaacc agctcttgaa tcctgagaag
     7441 cgccgagatg ggtataacat cggcaggtat gcaaagcaga gatgcagagt gcggggaacg
     7501 aatcttcacc agaacggtga gacaggttaa gcagcagact acggttcatt atttcgtatc
     7561 acctccacgg ccgcctgtta agacgaaccc acaagccaaa actctgattt caacccggct
     7621 ggaagtggca acacgaaaga aacgtcgtgt gctttttatt taagccgcgc gccgcgtttt
     7681 ataccccaca atggcagaaa attgcaaaat ataaatacgc agaatgccgg cattgtcagg
     7741 aaaaatttcc agccacgttt ttaacagaat gagacacgat tcaaaaaaaa gtggaaatcg
     7801 ggtgaagaat tgacctaaaa tagccatcca gatgttaatc catccatacc gattaacact
     7861 cagactgcca gtgtttttaa tctgcagagt cgtggtagga tccgctacca cagaaaatcc
     7921 acacaacagt ttgagctaac caaattctct ttaggtgata ttaaatatgg caaaacacct
     7981 ttttacgtcc gagtccgtct ctgaagggca tcctgacaaa attgctgacc aaatctctga
     8041 tgccgtttta gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta
     8101 cgtaaaaacc ggcatggttt tagttggcgg cgaaatcacc accagcgcct gggtagacat
     8161 cgaagagatc acccgtaaca ccgttcgcga aattggctat gtgcattccg acatgggctt
     8221 tgacgctaac tcctgtgcgg ttctgagcgc tatcggcaaa cagtctcctg acatcaacca
     8281 gggcgttgac cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg
     8341 ctacgcaact aatgaaaccg acgtgctgat gccagcacct atcacctatg cacaccgtct
     8401 ggtacagcgt caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc gcccggacgc
     8461 gaaaagccag gtgactttcc agtatgacga cggcaaaatc gttggtattg atgctgtcgt
     8521 gctttccact cagcactctg aagagatcga ccagaaatcg ctgcaagaag cggtaatgga
     8581 agagatcatc aagccaattc tgcccgctga atggctgact tctgccacca cattcttcat
     8641 caacccgacc ggtcgttttg ttatcggtgg cccgatgggt gactgcggtc tgactggtcg
     8701 taaaattatc gttgatacct acggcggcat ggcacgtcac ggtggcggag cattctctgg
     8761 taaagatcca tcaaaagtgg accgttccgc agcctacgca gcacgttatg tcgcgaaaaa
     8821 catcgttgct gctggcctgg ccgatcgttg tgaaattcag gtttcctacg caatcggcgt
     8881 ggctgaaccg acctccatca tggtagaaac tttcggtact gagaaagtgc cttctgaaca
     8941 actgactctg ctggtacgtg agttcttcga cctgcgccca tacggtctga ttcagatgct
     9001 ggatctgctg cacccgatct acaaagaaac cgcagcatac ggtcactttg gtcgtgaaca
     9061 tttcccgtgg gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg gtctgaagta
     9121 atctttcttc acctgctttc aaaggccagc ctcgcgctgg cctttttctt ttacctcacc
     9181 gcggtggata ggcattcacg ccgcatccgg caaaaagctg cccgcacaat aacatcattc
     9241 ttcctcaaca cgttttatcg caaattatca tcactactga aaccgattac accaataaca
     9301 ccagccaaat atttgtaatg ttttcatatt attattcagt tttcacagtt gttacatttc
     9361 ttttcagtaa agtcttaatt gcagataaca gcgtttaatc tatgatgata taactcaatt
     9421 attttcatgc acttaaacca taactaagat aaatgttagt gtaagcgatt acactaatgt
     9481 gatttgcttc acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac
     9541 catattggag ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac
     9601 gtttttcgtc tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt
     9661 aattgctggc gcactgccgt ttattgcaga tgaattccag attacttcgc acacgcaaga
     9721 atgggtcgta agctccatga tgttcggtgc ggcagtcggt gcggtgggca gcggctggct
     9781 ctcctttaaa ctcgggcgca aaaagagcct gatgatcggc gcaattttgt ttgttgccgg
     9841 ttcgctgttc tctgcggctg cgccaaacgt tgaagtactg attctttccc gcgttctact
     9901 ggggctggcg gtgggtgtgg cctcttatac cgcaccgctg tacctctctg aaattgcgcc
     9961 ggaaaaaatt cgtggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct
    10021 cggtgcttat ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg
    10081 tgtgattatc atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc
    10141 acgttggttt gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg
    10201 ttacaccagc gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa
    10261 acagagtggc tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg
    10321 cgtactgtta caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc
    10381 gaaaatcttc gaactggcgg gttataccaa cactaccgag caaatgtggg ggacagtgat
    10441 tgtcggcctg accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg
    10501 acgtaaacca acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg
    10561 tacaatgatg catatcggta ttcactctcc gtcagcgcag tatttcgcca tcgccatgct
    10621 gctgatgttt attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc
    10681 cgaaattcag ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg
    10741 gattgccaac atgatcgttg gcgcaacgtt cctgaccatg ctcaacacac tgggtaacgc
    10801 caacaccttc tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct
    10861 ggtaccggaa accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg
    10921 taaactgcgc gaaataggcg ctcacgatta atctccccaa gcttcctccc atcgcggagg
    10981 aagccacctc ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc
    11041 gtctccctat cgccatccaa caggccgtta tgcgtcgcct gcgggaaaaa ctcgcccagg
    11101 ctaacctgaa attggggcgt aactacccgg agccaaaact ctcttatacc cagcgcggca
    11161 cctccgccgg aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt
    11221 tggaaaacag tgaagccttt attgaagaag tggtgccgca cgaactggca catttgctgg
    11281 tatggaaaca tttcgggcgc gtagcaccac atggcaaaga gtggaagtgg atgatggaaa
    11341 acgtgctggg tgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca
    11401 acaccttccc ctaccgctgc aagtgccagg agcatcagct taccgtacgc cgccataatc
    11461 gcgtagttcg tggcgaagcc gtctatcgct gtgttcactg cggcgaacaa ctggtcgtaa
    11521 aataaccatc tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact
    11581 ttcgctacgt tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt
    11641 gctgcggtgg tactgagcgc agcattttcc ggcccggcgt tggccgaagg tatcaatagt
    11701 ttttctcagg cgaaagccgc ggcggtaaaa gtccacgctg acgcgcccgg tacgttttat
    11761 tgcggatgta aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc
    11821 tatcaggtgc gcaaaaatga aaaccgagcc agccgcgtag agtgggaaca cgtcgttccc
    11881 gcctggcagt tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct
    11941 aaagatccgg tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggt
    12001 gaggtgaatg gcgatcgcgg caactttatg tacagccagt ggaatggcgg cgaaggccag
    12061 tacggtcaat gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt
    12121 gcacgcggtg ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc
    12181 tctcgccagc aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg
    12241 gagtgcgagc gcgatgaacg catcgcgaag gtgcaaggca atcataaccc gtatgtgcaa
    12301 cgcgcttgcc aggcgcgaaa gagctaacct acactagcgg gactcttttt gttaacccct
    12361 accccacgcg tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg
    12421 catttatcat cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc
    12481 caaccatatc gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg
    12541 tagcaaccag gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa
    12601 ggtgctggaa ggcaagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt
    12661 gatgtcgcgt ggtgaaaaaa tggaatttac gatccagaaa tcgatcgaac tcggtgtaag
    12721 cctcattacg ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa
    12781 caagaagctc cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa
    12841 ccgggtgccg gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga
    12901 aggactgaaa ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgccgttacc
    12961 ggttgaacgc gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc
    13021 catgactgcc cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga
    13081 gacaactgcg ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg
    13141 gagaagaata atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa
    13201 gaaagattcc agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta
    13261 tatggagatg ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cctgcacgct
    13321 gaacgtgaag cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct
    13381 ggccgatctc gatgtgctcc tgatgcgtaa agacccgccg tttgataccg agtttatcta
    13441 cgcgacctat attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca
    13501 gagcctgcgc gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga
    13561 aacgctggtt acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat
    13621 cattcttaag ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga
    13681 tccaaacctc ggcgtgattg ccgaaaccct gactgagcat ggcactcgct actgcatggc
    13741 gcaaaattac ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga
    13801 gccggtaccg tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc
    13861 tgccggtggt cgcggtgaac ctcggccgct gacggaaagt gactggaaaa tcgcccatca
    13921 gatcgggccg acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga
    13981 ccgtctgact gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt
    14041 tccggtgtcg atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta
    14101 acccacctta gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg
    14161 catactgggc gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca
    14221 gcatcacttt cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt
    14281 ctacatttgc gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga
    14341 aaatctcaaa attgaaggga ttctggaaaa gctgaagatc acgccggagc ggcgtgatga
    14401 atcaatccgt ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt
    14461 tattttgcat actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt
    14521 gatgaccact tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt
    14581 attggtggct ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga
    14641 taacgcgtgg ctaacggccc cggcggatct gaatattttg ttcaaaacgc cgattgccga
    14701 ccgctggcgc gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc
    14761 agggcacgcc tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc
    14821 gtagcggtcg gccaacgcat taccggcact gctcgccctt tgcctgcaat taaagcacag
    14881 gacggtacgc cggactggaa cctcatcgaa cgtttactga aagagtggca gccggacgaa
    14941 atcatcgtcg gtttgccgct gaatatggac ggcaccgagc aaccattgac tgccagagcg
    15001 cgtaaatttg ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgaa
    15061 cgtcttagca ccgtggaagc ccgttccggt ctgtttgaac agggcggcta tcgggcgctc
    15121 aacaaaggca aagtcgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag
    15181 ggatattaag gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg
    15241 gcgtgaacgc cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta
    15301 gcgtcgcatc agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc
    15361 aacgtcacaa acgcccttcc cccacccgct gctgataact ctgctgaaac gttatcatcc
    15421 ccacctgctg cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca
    15481 aattccccac cgcgggagtg ttaatcaata attcaaacag cgccacgcgt ccttcctgtt
    15541 tatccacttc cagcttttgt gacaacacgg cccgtaaact acctgccagt tgattacgta
    15601 cgggatcttt ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac
    15661 cacgcgtatg taatgttgcc agcaccaaat gcccggtttc tgccgccgtc agtgccagac
    15721 ggattgtctc gctgtcacgc agctctccga gcaaaatcac atcaggatct tcccgcaatg
    15781 cggcccgcaa tcccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca
    15841 aacatcgctg gctggcatag atatattcaa caggatcttc cagcgtcaga atatgcgcat
    15901 cggcatgttg attgagatag ccaaccatcg ccgccagcgt ggtagatttg ccgctccccg
    15961 tcgcccccgt caccagaatc aggccattct cgctcttgag taattccggc aatatcgttg
    16021 gtgcgccaag ctgttcgagc tgcgggcagt gcgaaggtaa cagccgtaac gccagcgaaa
    16081 tgccatgccg ttgcgcgaat gcgctgccac gcaatcgctg gttttccgcc agcgacacgg
    16141 caaaatccag ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc
    16201 gcagtagctc ttcgacgtcc ggcgcatcaa acggcgcagc ttccattagc ccgcgaatgc
    16261 gccatcgtgc gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa
    16321 gggccacaat ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat
    16381 aacctggcac aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca
    16441 gaagaaatta cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc
    16501 attgatgccg ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt
    16561 cgccactttc aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag
    16621 tctaataaaa gccgcctggt ggcagagcat ttcgactggt gtcataccat cgaccgtttg
    16681 cgcattgcta cccgcctcaa cgatcagcgc ccggcagaac ttccccctct taacgtactg
    16741 attcaaatta atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac
    16801 gagctggcag cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc
    16861 cctgcgcctg agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca
    16921 tttgccggac tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac
    16981 gatatggaag ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt
    17041 ggtgcgcgtg attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt
    17101 tcctgctttc aacggtcatt gagctgtata ccatggtgct gttattacgc atctggatgc
    17161 agtgggctca ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtaacgcagc
    17221 caattatcgg gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc
    17281 tgctggttgc ctatattctc agttttatca aagccattgt gctgtttaaa gtggtgacct
    17341 tcctgccaat catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga
    17401 ttttctgggt cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga
    17461 ttgaatacgt gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac
    17521 cggcaatggg tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgtcatca
    17581 atatgggtgt cgcagaagta ttacaggcaa ccggaaacat cctgctgccg gggctgtgga
    17641 tggcgttatg aatgccgtaa cagttaatga tgacggtctg gttttacggc tctatattca
    17701 gccgaaagcc agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat
    17761 taccgcgccg ccagttgacg gccaggccaa cagtcatctg gtgaagtttc tcggtaagca
    17821 attccgggtt gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca
    17881 aattaaaatc attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt
    17941 atcctatgca aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt
    18001 cgctgcttag cgactttggt cttgatatcg tggcacaaac agacctcggc gttgattccg
    18061 ctgaagaaac cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa
    18121 aagtgaccgg tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg
    18181 gcgcgccggg tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc
    18241 tgcaaaaact gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcacgtttcc
    18301 actgcgtgct ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca
    18361 gctggccggg cgtgattact cgcgaaccag cgggcactgg tggctttggt tatgatccaa
    18421 tcttcttcgt accttccgaa gggaaaacag ccgccgaact gacccgcgaa gaaaagagcg
    18481 ccatttccca ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa
    18541 ttaccgccgc tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc
    18601 gatttcaact ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg
    18661 ctttgcgatc tggacaatga tgtggcttac gctcagggcc gtaaagtaaa gacaatcttt
    18721 attggcggtg gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctggacggc
    18781 gtgcgtgcgc gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaaccctggt
    18841 acggtagaag ccgatcgctt tgtcgattat cagcgtgctg gtgtgaaccg catctctatt
    18901 ggtgtgcaga gttttagcga agaaaagctg aaacgacttg ggcgcattca tggcccgcaa
    18961 gaagcgaaac gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat
    19021 ttgatgcatg gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc
    19081 attgaactga atccgccgca tctttcctgg tatcaactga ccatcgaacc caatacgctg
    19141 tttggttcgc gaccaccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag
    19201 gggcatcagt tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa
    19261 cccggttatc agtgccagca caatctcaac tactggcgat ttggtgacta catcggtatt
    19321 ggctgcggcg cgcacggcaa agtcaccttc ccggatgggc gcattctgcg taccactaaa
    19381 acgcgtcatc ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa
    19441 gccgcagata agccgtttga gttctttatg aaccgctttc gcttgctgga ggccgcgcca
    19501 cgcgcagagt ttagtgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac
    19561 gaggcgattg cccagggtta tctcacagaa tgtgcggatt actggcagat aacggaacat
    19621 gggaagctgt ttttaaattc gctgctggag ctttttctgg ctgagtaaac ttgtattgcc
    19681 ggatgcggcg tgaacgcctt atccagccta catgtggcaa cggttgtagg tctgataaga
    19741 cgcgcaagcg tcgcatcaga cgttgattgc cggatgcggg gtcaacgccg catcctgcta
    19801 caaatcgtgc actatatcaa acttacttaa tcaccccatt gcgccgcagt tgttcggtat
    19861 cttttttcca gcgttcatta gcttggttga tatcgtcaat tttcatcaaa atcgcgactt
    19921 ttttactgtt tatttctgaa actttcatcg ttaacggata ttgccctttt tcgagcgtta
    19981 cccagtcatc ctgcaattta cggctgagtt tttgtgattg cagaaaagtc tgtcgccgtt
    20041 gttcgttgat atcgaaatct cgtttctgct gaatcggcac ttcataacac tcttcactct
    20101 gtttatgaca tgccgcaatc gccggttgat aaacctcttt gtcatattta accgcgaacg
    20161 cctcgctatc gttctgttta ttaaact
//
LOCUS       DAJTNA010000065        19628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTNA010000065 DAJTNA010000000
VERSION     DAJTNA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19628
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.066"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(98..910)
                     /locus_tag="OO161_001966"
     CDS             complement(98..910)
                     /locus_tag="OO161_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5463041.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            1172..1933
                     /gene="udp"
                     /locus_tag="OO161_001967"
     CDS             1172..1933
                     /gene="udp"
                     /locus_tag="OO161_001967"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5463042.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2074..3501
                     /gene="rmuC"
                     /locus_tag="OO161_001968"
     CDS             2074..3501
                     /gene="rmuC"
                     /locus_tag="OO161_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5463043.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3596..4351
                     /gene="ubiE"
                     /locus_tag="OO161_001969"
     CDS             3596..4351
                     /gene="ubiE"
                     /locus_tag="OO161_001969"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5463044.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4365..4970
                     /gene="ubiJ"
                     /locus_tag="OO161_001970"
     CDS             4365..4970
                     /gene="ubiJ"
                     /locus_tag="OO161_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5463045.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            4967..6607
                     /gene="ubiB"
                     /locus_tag="OO161_001971"
     CDS             4967..6607
                     /gene="ubiB"
                     /locus_tag="OO161_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5463046.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6686..6955
                     /gene="tatA"
                     /locus_tag="OO161_001972"
     CDS             6686..6955
                     /gene="tatA"
                     /locus_tag="OO161_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5463047.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDSDFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            6959..7474
                     /gene="tatB"
                     /locus_tag="OO161_001973"
     CDS             6959..7474
                     /gene="tatB"
                     /locus_tag="OO161_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5463048.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7477..8253
                     /gene="tatC"
                     /locus_tag="OO161_001974"
     CDS             7477..8253
                     /gene="tatC"
                     /locus_tag="OO161_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5463049.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8295..9077
                     /gene="tatD"
                     /locus_tag="OO161_001975"
     CDS             8295..9077
                     /gene="tatD"
                     /locus_tag="OO161_001975"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5463050.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(9074..9562)
                     /gene="rfaH"
                     /locus_tag="OO161_001976"
     CDS             complement(9074..9562)
                     /gene="rfaH"
                     /locus_tag="OO161_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5463051.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9729..11222
                     /gene="ubiD"
                     /locus_tag="OO161_001977"
     CDS             9729..11222
                     /gene="ubiD"
                     /locus_tag="OO161_001977"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5463052.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11268..11969
                     /gene="fre"
                     /locus_tag="OO161_001978"
     CDS             11268..11969
                     /gene="fre"
                     /locus_tag="OO161_001978"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5463053.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12161..12979)
                     /locus_tag="OO161_001979"
     CDS             complement(12161..12979)
                     /locus_tag="OO161_001979"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HCR5463054.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARMHG
                     ISREMQDAFAARSHARTWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVE
                     ALATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGC
                     DPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKIN
                     LNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(12989..15178)
                     /gene="fadB"
                     /locus_tag="OO161_001980"
     CDS             complement(12989..15178)
                     /gene="fadB"
                     /locus_tag="OO161_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5463055.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIVKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15368..16699
                     /gene="pepQ"
                     /locus_tag="OO161_001981"
     CDS             15368..16699
                     /gene="pepQ"
                     /locus_tag="OO161_001981"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5463056.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            16699..17313
                     /locus_tag="OO161_001982"
     CDS             16699..17313
                     /locus_tag="OO161_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5463057.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPATKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17352..18803
                     /gene="trkH"
                     /locus_tag="OO161_001983"
     CDS             17352..18803
                     /gene="trkH"
                     /locus_tag="OO161_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5463058.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            18815..19360
                     /gene="hemG"
                     /locus_tag="OO161_001984"
     CDS             18815..19360
                     /gene="hemG"
                     /locus_tag="OO161_001984"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5463059.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
BASE COUNT         4570 a         4960 c         5368 g         4730 t
ORIGIN      
        1 atctccaatc aattcatact gattattttt tgggggacag agatggcagc aaaacgcact
       61 cgttcactca ccatctcaaa tatagactaa gaggaaacta actccgcttc ccaccatact
      121 gcttaaacca ttccagcata cgctgccagc catctttcgc agattcggca tgatagctcg
      181 ggcgataatc agcgttgaat gcatgcccgg cgtccgggta cacgataatc tctgctttcg
      241 cattagcagc ccgcagcgcc tggcgcatgg tttcaacgct ctcctgcgga atgctgttat
      301 cctgaccacc atataagccg agaaccggcg cgttaagatc ggttgcgata tcaacaggtt
      361 gtttcggtga attcagcgac ttgtcgcccg tcagtttgcc gtaccacgcc actgcggctt
      421 ttagctgtgg attatgcgcg gcatacagcc aggtgatacg tccaccccag cagaatccgg
      481 tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg acatgatcga
      541 gatcggccag cacctgcgag tcaggcactt ttgctaccag accgctaagc aacgtgggga
      601 tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg atagccagat
      661 acccctccag cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg ccaaaaattt
      721 cctgcactac aatgaccact ggcagtgggc catcgctttg ctttggtctg gcatggtaag
      781 caggcatgtt atccccttgt gaagggatag aggtgaagcc cgccacaatc gcgtcgtccg
      841 gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca gattgttgtg
      901 ttgttgccat ggtattctcc gtacccttat aaaaatgttg cgcaatgtta actatagtca
      961 gcatgcaaca aatcacattg cctgaatcgg ctcatctttt atgcagccct gcagaatgaa
     1021 gagtgattta tgtgatttgc atcacttttg gtgggtaaat ttatgcaacg catttgcgtc
     1081 atggtgatga gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt tttgcttctt
     1141 ctgactaaac cgattcacag aggagttgta tatgtccaag tctgatgttt ttcatctcgg
     1201 cctcactaaa aacgatttac aaggggctac gcttgccatc gtccctggcg acccggatcg
     1261 tgtggaaaag atcgccgcgc tgatggataa gccggttaag ctggcatctc accgcgaatt
     1321 caccacctgg cgtgcagagc tggatggtaa acctgttatc gtctgctcta ccggtatcgg
     1381 cggcccgtct acctctattg ctgttgaaga gctggcacag ctgggcattc gcaccttcct
     1441 gcgtatcggt acaacgggcg ctattcagcc gcatattaat gtgggtgatg tcctggttac
     1501 cacggcgtct gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg aattcccggc
     1561 tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct gcgaaatcca ttggcgcgac
     1621 aactcacgtt ggcgtgacag cttcttctga taccttctac ccaggccagg aacgttacga
     1681 tacttactct ggtcgcgtag ttcgtcactt taaaggttct atggaagagt ggcaggcgat
     1741 gggcgtaatg aactatgaaa tggaatctgc aaccctgctg accatgtgtg caagtcaggg
     1801 cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc acccagcaag agatcccgaa
     1861 tgctgagacg atgaaacaaa ctgaaagcca agcggtgaaa atcgtggtgg aagcggcgcg
     1921 tcgtctactg taattctctt ctcctgtctg aaggccgacg cgttcggcct tttgtatttt
     1981 tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgc ttgtacagca caattctatt
     2041 ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt tctcaatcat ggtttacgca
     2101 gttattgcgt tggtgggtgt ggcaattggc tggctgtttg ccagttatca acatgcgcag
     2161 caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag cgcggcaaaa
     2221 caacaaatta cccaaagcga gcactggcgt gcagagtgcg agttactcaa taacgaagtg
     2281 cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt aaccacgcgg
     2341 atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgattaa cagcgagcag
     2401 cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag caatcgccgg
     2461 gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg tgaacaactg
     2521 gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga acgccatacc
     2581 ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca ggaagcgatc
     2641 aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg cgaggtagta
     2701 ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga aacccaggtc
     2761 agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg gctgccgcag
     2821 ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg ctattttaac
     2881 gccgaagacg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc ggtgcgtaac
     2941 catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg aactctggat
     3001 tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga ccgccagccg
     3061 gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac tacgctgctg
     3121 gtggcgttgc gtactattgc caacctgtgg cgttatgagc atcaaagccg caacgcccag
     3181 caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcat cgatgacatg
     3241 tccgcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc aatgaaaaaa
     3301 ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg tttaggagta
     3361 gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca ggatgaagag
     3421 tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg cgatgatgaa
     3481 tataatcagc agtcgcgcta gcccattagg ggtagttaag ccgggtagaa atctagggca
     3541 tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca ttgagatggt
     3601 ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg aacaaaaagc
     3661 ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca tgaatgattt
     3721 gatgtcattt ggtattcatc gtttgtggaa gcgattcacg attgattgca gcggcgtacg
     3781 ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag cgaaattctc
     3841 ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat ccatgctcaa
     3901 aatgggacgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt atgttcaggc
     3961 gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca tttcgtttgg
     4021 tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg tgctgaaacc
     4081 cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc tgagcaaagc
     4141 ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg cgaacgacgc
     4201 cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg ataccctgaa
     4261 agccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga cggcaggggt
     4321 tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct tttaaacctt
     4381 tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca cccgcgctga
     4441 aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa ggcttttcga
     4501 cgtcattgat tctggtgttc agcgaacgcc aggttgatgt actgggcgaa tgggcaggcg
     4561 atgctgactg caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc gatcgccagc
     4621 agcttaccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt caggtggtgc
     4681 aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg ctggcccctt
     4741 ataccggtga tatcgccgct gaaggaatca gcaaagccat gcgcggaggc gcaaagttcc
     4801 tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact gaagagtggc
     4861 gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct gccgtcgagc
     4921 gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga cgccaggtga
     4981 agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg atgaactgat
     5041 ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat tctggatgcc
     5101 aaatcggcat aaagacaaac ttttaggtga gcgactacga ctggccctgc aagaactggg
     5161 gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt ttccaccgca
     5221 tattgccgat cagctggcgt tattgcagga caaagttgct ccgtttgatg gcaagctggc
     5281 gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgtggt ttgacgattt
     5341 tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc gattgaaatc
     5401 gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg tcattaaagc
     5461 ggatctgaaa cttatctacc gtctggctcg ctgggtgccg cgtttgctgc cggatggtcg
     5521 ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg atgaactgaa
     5581 tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag acagcccgat
     5641 gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg tgatggagcg
     5701 catttacggc attccggtgt ctgatgttgc ggcgctggag aaaaacggca ctaacatgaa
     5761 attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg acagcttttt
     5821 ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg aaaacccgaa
     5881 atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata aacgctatct
     5941 ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag agctacacgt
     6001 cgattcaggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg ccattcgtac
     6061 ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac atgtactgtt
     6121 aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac tggtgttact
     6181 ccagaaaacc ctgctctatg tcgaaggggt aggacgccag ctttatccgc agctcgattt
     6241 atggaaaaca gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg gtattcctgc
     6301 gctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc cagaactgcc
     6361 tgaattggtt tacgacagtt tgcgccaggg caagtattta cagcacagtg ttgataagat
     6421 tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt ttctcggaat
     6481 tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg agtgggggct
     6541 gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt ggcgcaaaac
     6601 acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc tttgtttaat
     6661 catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt tattgattat
     6721 tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg gttccgatct
     6781 tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa agcaggataa
     6841 aaccagtcag gattctgatt ttactgcgaa aactatcgcc gataagcagg cggatacgaa
     6901 tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg tgtaatccgt
     6961 gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg tcgttctggg
     7021 gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg cgttgcgttc
     7081 actggcgaca acggtgcaga acgaactgat ccaggagtta aaactccagg agtttcagga
     7141 cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac tgaaagcgtc
     7201 gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg caaacgatcc
     7261 agaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag ataatgaagc
     7321 tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc cggaacagaa
     7381 gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac cgaaaaccgc
     7441 tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga tactcaaccg
     7501 cttatcacgc atctaattga gctgcgtaag cgtctgctga actgcattat cgcggtgatc
     7561 gtgatattcc tgtgtctggt ctatttcgcc aatgacatct atcacctggt atccgcgcca
     7621 ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc ctcgccgttc
     7681 tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc ggtgattctc
     7741 tatcaggtgt gggcatttat cgccccagcg ctgtataagc atgaacgtcg cctggtggtg
     7801 ccgctgctgg tttccagctc tctgctgttt tatatcggca tggcattcgc ctactttgtg
     7861 gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt gcaggtatcc
     7921 accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt tggtgtctcc
     7981 tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc gccagaagac
     8041 ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat gttgctgacg
     8101 ccgccggatg tcttctcgca aacgctattg gcgatcccga tgtactgtct gtttgaaatc
     8161 ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga ggaaaacgac
     8221 gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag ggcggttgtc
     8281 atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa tttgcgaaag
     8341 accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta ctcatcaccg
     8401 gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat tcgtcctgtt
     8461 ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg actgaagaag
     8521 cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt ggtctcgact
     8581 ttaaccgcaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgct cagctacgca
     8641 ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac gagcggttta
     8701 tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat tgctttaccg
     8761 gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc attaccggtt
     8821 gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg attccggcgg
     8881 agaaattgct gatcgaaact gatgcgccgt atctgctccc gcgcgatttc acgccaaagc
     8941 catcatcccg gcgcaacgag ccagcccatc tgcaccatat tttgcaacgt attgcgcact
     9001 ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc aaaacactgt
     9061 ttgggattgc gttttagagt ttgcggaact cggtattctt cacactgtgc ttaatctctt
     9121 tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg gtgaaaatgg
     9181 cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga taaggggttg
     9241 ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaatcacc gccgatggga
     9301 ctatcgctgg cgacgcgcca aagcgcacga agtggctgac accgcgggtc gcgttgatag
     9361 tcgtggtatg aatcacttct ggatcaaatt cgacaaacag gtagttggga aacaatggct
     9421 cactgactgc agtacgtttt ccacgcacga ttttttccag ggtgatcatc ggtgccaggc
     9481 aattcacagc ctgtctttcg aggtgttcct gggcacgttg aagttgcccg cgcttgcagt
     9541 acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg caaggatagc
     9601 aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc agagttcacg
     9661 ctaatttaac aaatttacag catcgcaaag atgaacgccg tataatgggc gtagattaag
     9721 aggctacaat ggacgccatg aaatataacg atttacgcga cttcttgacg ctgcttgaac
     9781 agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa atcactgaaa
     9841 ttgctgaccg cactttgcgt gccggtgggc ctgcgctgtt gttcgaaaac cctaaaggct
     9901 actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg atgggcatgg
     9961 gacaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt ctgaaagagc
    10021 cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt aagcaagtat
    10081 tgaacatgcc gacaaagcgg ctgcgtggtg tgccctgcca acaaaaaatc gtctctggcg
    10141 atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat gccgcaccgc
    10201 tgattacctg ggggctgaca gtgacgcgcg gcccgcataa agagcggcag aatctgggca
    10261 tttatcgcca gcagctgatt ggtaaaaaca aactgattat gcgctggctg tcgcatcgcg
    10321 gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt ttcccggttt
    10381 ctgtggcgct gggtgccgat cccgccacga ttctcggtgc agtcaccccc gttccggata
    10441 cactttcaga gtatgcgttt gccggattgc tgcgcggtac caagaccgaa gtggtgaagt
    10501 gtatctccaa tgaccttgaa gtgcccgcca gtgcggagat tgtgctggaa gggtatatcg
    10561 aacaaggcga aactgcgccg gaagggccgt atggcgacca caccggttac tataacgaag
    10621 tcgatagttt cccggtattt accgtgacgc atattaccca gcgtgaagat gcgatttacc
    10681 attccaccta taccgggcgt ccgccagatg agcccgcggt actgggcgtg gcgttgaacg
    10741 aagtgtttgt accgattctg caaaaacagt tcccggaaat tgtcgatttt tacctgccgc
    10801 cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac gccggacacg
    10861 cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac actaaatttg
    10921 tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt tgggcgatta
    10981 ccacccgtat ggacccggcg cgggatactg ttctggtaga aaatacgcct attgattatc
    11041 tggattttgc ctcgcctgtc tccgggctgg gttcaaaaat ggggctggat gccacgaata
    11101 aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat ccagatgttg
    11161 tcgcgcatat tgacgccatc tgggatgaac tggctatttt taacaacggt aaaagcgcct
    11221 gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg acaaccttaa
    11281 gctgtaaagt gacctcggta gaagctatca cggataccgt atatcgtgtc cgcatcgtgc
    11341 cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg gatgagcgcg
    11401 acaaacgtcc gttttcaatg gcttcgacgc cggatgaaaa agggtttata gagctgcata
    11461 ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc ctcaaagatt
    11521 atcaaatcgt ggtcgatatt ccctacggag aagcgtggct gcgcgatgat gaagagcgtc
    11581 cgatgatttt gattgcgggc ggcaccgggt tctcctatgc gcgctcgatt ttattgacgg
    11641 cgttggcgcg taatcccaat cgcgatatca ccatttactg gggtgggcgt gaggagcaac
    11701 atctttatga tctctccgag cttgaggcgc tttcgctgaa gcatcctggc ctgcgtgtgg
    11761 tgccggtggt tgaacaaccg gaagcgggct ggcgtgggcg tactggcacc gtgttaacgg
    11821 cggtcttgca ggatcatggt acgctggcag agcatgatat ctatattgcc ggacgttttg
    11881 agatggcgaa aattgctcgt gacctgtttt gcagtgagcg taatgcgcgg gaagatcgtc
    11941 tgtttggcga tgcgtttgca tttatctgag atataaaaaa aaccgcccct gacaggcggg
    12001 aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat ccggcaatcg
    12061 gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg tgcaggatcg
    12121 gtagaccgga taagacgttc acgccgcatc cggcaagtgg ttaaacccgc tcaaacaccg
    12181 tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactga acgtctttgc
    12241 gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacagccc agcggatgac
    12301 ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc aatctgctcc attagcccga
    12361 gatctttaat gcacggcagg atctgcgcgg caaaggcttc gttcatctca aacacgccga
    12421 tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga accgggccgt
    12481 aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga gcgcgcggct
    12541 taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct gcgccatcgg
    12601 aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac gccggacgca
    12661 gcgtggcgag ggcttccacg gtggtttccg gtcgaatcac ctcgtcgtaa ttaaactgct
    12721 tcaggacgcc gtcggcatcg tgaccaccgg tcgggatgat ttcattttta aatgcgcccg
    12781 actgcgtggc ggcccaggtg cgggcgtgtg accgcgcggc aaaggcatcc tgcatttcac
    12841 ggctgatacc gtgcatacgc gccagcaggc tacgcattaa atgagcggag agatcttctg
    12901 cacgcacgtt acgaaaagca ccgcccttcg aacggcccat cggggtgcga attgcatcga
    12961 caatgacaac ctgttccatt gtgactcctt aagccgtttt caggtcgcca accggacggg
    13021 ctggctcaac cggaggatag tacggttcgt tatgacgcgc tttattacgc agaccttccg
    13081 gcacttcata cagcgggccg aggtgctgat attgctgtgc catatcgagg tattttgcgc
    13141 taccgagggt gtccagccag cggaacgcgc cgccgtggaa cggagggaag cccaggccgt
    13201 agaccagcgc catatccgct tccgccggag tggcgataat gccctcttcc agacagcgga
    13261 ccacttcgtt gaccatcggg atcatcatgc gggcgataat ctcttcttcg ctgaaatcgc
    13321 gcttcggctg gctcacttct gccagcaggt cttcaacggc ggcgtcttct tctttcttcg
    13381 gcttaccttt gctgtcttct ttataacgcc agaaaccgag gccgttcttc tgaccaaagc
    13441 ggttggcatc aaacagcgcg tcgatggcat cgcggtaatc tttctgcatc cgctgcggga
    13501 agcctgctgc catgacagcc tgagcgtgat gcgcggtatc aatgcccacg acatccagca
    13561 gatatgccgg acccataggc cagccaaact gtttttccat cactttgtcg atcttgcgga
    13621 aatccgcgcc gtcgcgcagc agctggctga aaccggcgaa atacgggaac agcacgcggt
    13681 taacaaagaa gccggggcag tcgttaacca caatcggcgt cttgcccatc ttgctcgccc
    13741 aggcgacaac tttcgcgatg gtttcgtcgg agcttttctc gccgcgaata atttctacca
    13801 acggcatccg gtgtaccggg ttaaaaaagt gcattccgca gaagttttcc gggcgttcca
    13861 gcgcgttggc cagttcgctg ataggaatgg ttgaagtgtt agacgccagc acggtatccg
    13921 ggcgtacttt ttgttcggtt tctgccagta cggctttttt cactttcggg ttttcgacaa
    13981 ccgcttctac cacaacatcc acgcggtcaa acccggcgta gtcgagcgtt gggtggatcg
    14041 tggagatcac gccagccagt ttcagaccat cgatcttgcc gcgttcaagc tgcttgttca
    14101 gcagtttcgc ggcttcggtc atgccgaggg ttaacgactt gtcgttgata tctttcatga
    14161 caaccggtac gcctttccac gcagactggt aagcaatgcc gccacccatg atgcctgcgc
    14221 ccagtacagc ggcctgtttc ggtgtttcaa cgtctttggt gagtttcttc gctttgcctt
    14281 ttacatattg atcgttaagg aaaatgccga ccagtgcgcg ggcttcgttg gtgtgcgcca
    14341 gcgggacaaa gcttttgttt tccagattta atgcttcttc acgaccaaaa cgggccgcag
    14401 cttcaatggt ttttactgcg gtgatgggcg ccggataatg tttccccgcc gtttgtgcga
    14461 ccatcccttt aacgatggtg aagctcatgg tggcttcaat cttgctcagt tttagtggtt
    14521 ccagcttcgg ctgacgtttt gctttccagt cgaggtcgcc gttaatggcc tggcgtaaaa
    14581 ccgcctttgc gccttcaacc agtttttctg ctttgactac gccatccacc agaccgattt
    14641 tcagcgcctg atccgcgccg acatctttac cggcggcaat gatttccagc gcactgtcag
    14701 cgcccagcat acgtggcata cgtacagaac cgccaaagcc cggcatgatg cccagtttgg
    14761 tttccggcag accgatgcgc agatccggcg tcgccagacg ataatcggtc gccagcacgc
    14821 attcgcagcc accacccagc gcatagccgt tgacggcagc aatggtcggc accggcagat
    14881 cttccaggcg attaaacacg ctattggcaa agtgcagcca ctgacttaac tgttcttcag
    14941 gaacgaggaa cagggacaaa aattcggtga tatcagcacc gacgataaag gctgctttgt
    15001 tcgaacgcag cagcagccct tttagatctg attgctgttc cagcacgccg atggcctcgc
    15061 cgaggctggc gacggtcgca gtgtcgagtt tattaactga acctggggca tcaaatacca
    15121 gttcggcaat gccatcttcc agccagtcaa ggtacagggt gtcgcctttg taaagcatgt
    15181 cagtctcctg aatccgcaag gtgatctggt cgtaccagat gcatcgaagt gtgtatttta
    15241 tgttaaaaat atgcaaataa aagattaaaa aaatgccgat ctgatcacgc tcggcagaaa
    15301 tcacgctctg gatgaacgat gtgctaagat gcggagactt aaggtcaaaa aaacagaagg
    15361 gtaaaaaatg gaatcactgg cctcgctcta taaaaatcat atagctacct tacaagaacg
    15421 gactcgcgat gcgctggcgc gcttcaagct ggatgcatta cttattcact ccggcgagct
    15481 gttcaacgtt tttctcgacg atcatcccta tccgtttaaa gtgaacccgc aattcaaagc
    15541 gtgggtgccg gtaactcagg tgccaaactg ctggttgctg gtggatggcg tgaacaagcc
    15601 gaaactgtgg ttctatctgc cggttgatta ctggcacaac gtcgaaccgc tgccgacctc
    15661 cttctggact gaagatgtgg aagtgatcgc actgccgaaa gccgatggca ttggtagcct
    15721 gctgcccgct gcgcgcggca atatcggtta tatcggtccg gtgccggaac gtgcgctgca
    15781 actgggtatt gaggccagca acatcaaccc gaaaggggtg atcgactacc tgcattacta
    15841 ccgctccttc aaaaccgagt acgaactggc ctgtatgcgt gaagcgcaga aaatggcggt
    15901 caacggtcat cgtgcggcag aagaagcgtt ccgttctggc atgagcgagt tcgatatcaa
    15961 catcgcctat ctgaccgcga ccggtcatcg tgataccgac gtaccttaca gcaacattgt
    16021 ggcgcttaac gaacacgctt cggtgctgca ttacaccaaa ctggatcatc aggcaccgga
    16081 agagatgcgc agcttcctgc tggatgccgg ggccgaatat aacggctatg cggctgacct
    16141 gactcgtacc tggtcggcaa aaagcgacaa cgactacgca cagctggtga aagacgtaaa
    16201 tgatgaacaa cttgcgctga tcgccaccat gaaagctggc gtcagctatg tggattacca
    16261 cctccagttc catcagcgca tcgccaaatt gctgcgtaaa catcaaatca tcaccgatat
    16321 gagtgaagaa gcgatggtcg aaaacgatct caccggaccg tttatgccgc acggtatcgg
    16381 ccatccgctg ggcctgcagg tgcatgacgt agccggtttt atgcaggatg atagcggtac
    16441 acacctcgcg gcaccggcaa aatatccgta cctgcgctgc acccgtattc tccagccggg
    16501 catggtgtta accatcgaac cgggtatcta cttcatcgaa tcgctactgg caccgtggcg
    16561 tgaagggcag ttcagcaagc acttcaactg gcagaaaatt gaagcactga aaccgttcag
    16621 cggcattcgt atcgaagaca acgtggtgat ccacgaaaat aacgtggaaa acatgacccg
    16681 ggatctgaaa ctggcgtgat ggaaagctgg ttaattcctg cggcaccggt cacggtcgtt
    16741 gaagagatca aaaagagccg cttcataacg atgttggcgc ataccgatgg cgttgaggcg
    16801 gcgaaagcgt ttgttgaatc ggtgcgggca gaacaccccg atgcccgcca ccattgcgtg
    16861 gcgtgggtgg cgggtgcgcc ggatgattct caacagctgg gtttctctga cgacggggag
    16921 ccggcgggaa cggcaggtaa accgatgctc gcccagctaa tgggcagcgg cgtcggggaa
    16981 attaccgctg tggtagtgcg ctactacggc ggcatattgc ttggcaccgg tgggttagtg
    17041 aaagcgtatg gcggcggcgt gaatcaggcg ctgcgccagc taacgaccca acgcaagacg
    17101 ccattaaccg aatatacttt gcaatgtgaa tatagtcagt taaccggcat tgaagcgttg
    17161 ctggggcagt gtgacggcaa aattatcaac agtgattatc aggcattcgt tctgctgcgg
    17221 gtggcgcttc cggcgacgaa agtggctgaa ttttccgcaa agctggcgga ttttagccgt
    17281 ggttcattgc aattgttagc gattgaagaa taatccccac ttcgttttgc agaactaagg
    17341 aagcggcaga gatgcatttt cgcgccatta cccgaatcgt tggactactg gtcatcttat
    17401 tttcagggac catgattatc cctgggctgg tagcactcat ctaccgggat ggagcgggcc
    17461 gcgcttttac ccagaccttt tttgtcgccc tcgccattgg ctctatgctg tggtggccga
    17521 accgcaaaga gaagggcgaa ctgaaatccc gtgaggggtt tctgatagtg gtgctgtttt
    17581 ggaccgtgct gggtagtgtc ggtgcgctcc cttttatctt ctcggaaagc ccgaacctca
    17641 cgattaccga tgcgtttttt gaatctttct ctggcctgac caccaccggg gccactacgc
    17701 tggtggggct ggattcgctc cctcatgcca tcctctttta tcgccagatg ctgcaatggt
    17761 ttggcgggat ggggatcatc gtgttagcgg ttgcgatact gcctatcctc ggcgtgggtg
    17821 ggatgcagct ctatcgcgca gaaatgcccg gcccgctgaa agataacaaa atgcgcccgc
    17881 gaattgcgga aacggcgaaa accctgtggt tgatttatgt cttgctgacc gtcgcctgtg
    17941 cgctggcgtt gtggtttgcc ggaatggatg cctttgatgc catcggccat agctttgcga
    18001 ctatcgctat tggcggcttc tcgacacatg atgccagtat cggttatttc gacagtccga
    18061 ctattaacac tatcattgct atcttcctgc tgatctccgg ttgtaactac ggtctgcact
    18121 tttcactgtt aagtgggcgt agtctgaagg tttattggcg cgatccggaa tttcgcatgt
    18181 ttatcggcgt acagtttacg ctggtggtta tttgtacact cgtactgtgg tttcataatg
    18241 tctacagttc ggcgctgatg acaattaacc aggcgttttt ccaggtggta tcgatggcga
    18301 caaccgccgg gtttacgact gacagcattg cccgctggcc gctctttttg ccggtactgc
    18361 ttttatgttc agcttttatc ggcggttgcg ccgggtcaac gggcggtggc ctgaaagtga
    18421 tccgcatcct gctgctgttt aagcagggga accgtgagct gaaacgactg gtgcatccga
    18481 acgccgtcta tagcattaag ctggggaatc gcgcactgcc ggaacgtatc ctcgaagccg
    18541 tttggggatt tttctccgcc tatgcattgg tgtttattgt cagtatgctg gcgattatcg
    18601 ccacgggcgt ggatgatttt tctgcctttg cctcagttgt tgcgacattg aataacctgg
    18661 ggccagggct tggcgtggtt gctgataact ttaccagtat gaacccggtg gctaaatgga
    18721 tcctgattgc caacatgctg tttggtcgtc tcgaggtctt tacattgctg gtgctcttta
    18781 ccccgacttt ctggcgtgaa tgatggagta atacgtgaaa acattaattc ttttctcaac
    18841 aagggacgga caaacgcgcg agattgcctc ctacctggct tcggaactga aagaactggg
    18901 gatccagacg gatgtcgcca atgtgcaccg cattgaagaa ccacaatggg aaaactatga
    18961 ccgtgtggtc attggtgctt ctattcgcta tggtcactac cactctgcgt tccaggaatt
    19021 tgtcaaaaaa catgcgacgc gactgaattc gatgccgagc gccttttatt ccgtaaacct
    19081 ggtggcgcgc aaaccggaga agcgtactcc acagaccaac agctacgcgc gcaagtttct
    19141 gatgagttca caatggcgtc ccgatcgctg cgcggtcatt gccggggcgc tgcgttaccc
    19201 acgttatcgc tggtacgacc gttttatgat taagctgatt atgaagatgt caggcggtga
    19261 aacggatacg cgcaaagaag ttgtctatac cgattgggag caggtggcga atttcgcccg
    19321 agaaatcgcc cagttaacca acaaaccgac gctgaaataa gcataaagaa taaaaaatgc
    19381 gcggtcagaa aattatttta aatttcctct tgtcaggccg gaataactcc ctataatgcg
    19441 ccaccactga cacggaacaa cggcaaacac gccgccgggt cagcggggtt ctcctgagaa
    19501 ttccgacaga gaaagcaaaa ataaatgctt gactctgtag cgggaaagcg tattatgcac
    19561 accccgcgcc gctgagaaaa agcgaagcgg cactgctctt taacaattta tcagacaatc
    19621 tgtgtggg
//
LOCUS       DAJTNA010000066        19572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTNA010000066 DAJTNA010000000
VERSION     DAJTNA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19572
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.067"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            6..81
                     /locus_tag="OO161_001985"
     tRNA            6..81
                     /locus_tag="OO161_001985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO161_001986"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO161_001986"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1226)
                     /locus_tag="OO161_001987"
     CDS             complement(468..1226)
                     /locus_tag="OO161_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5463060.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1234..2327)
                     /locus_tag="OO161_001988"
                     /pseudo
     CDS             complement(1234..2327)
                     /locus_tag="OO161_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(2337..3507)
                     /locus_tag="OO161_001989"
                     /pseudo
     CDS             complement(2337..3507)
                     /locus_tag="OO161_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3575..4600)
                     /locus_tag="OO161_001990"
     CDS             complement(3575..4600)
                     /locus_tag="OO161_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5463061.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRSVAAAVFGDDTKVKYTPLTAKERFTVLQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5031..5252)
                     /locus_tag="OO161_001991"
     CDS             complement(5031..5252)
                     /locus_tag="OO161_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463062.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5505..8609)
                     /gene="acrF"
                     /locus_tag="OO161_001992"
                     /pseudo
     CDS             complement(5505..8609)
                     /gene="acrF"
                     /locus_tag="OO161_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8621..9778)
                     /gene="acrE"
                     /locus_tag="OO161_001993"
     CDS             complement(8621..9778)
                     /gene="acrE"
                     /locus_tag="OO161_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR5463063.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAYVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10177..10839
                     /gene="acrS"
                     /locus_tag="OO161_001994"
     CDS             10177..10839
                     /gene="acrS"
                     /locus_tag="OO161_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR5463064.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIDWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10842..11021)
                     /locus_tag="OO161_001995"
     CDS             complement(10842..11021)
                     /locus_tag="OO161_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5463065.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(11105..11989)
                     /gene="yhdJ"
                     /locus_tag="OO161_001996"
     CDS             complement(11105..11989)
                     /gene="yhdJ"
                     /locus_tag="OO161_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5463066.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLILADPPYN
                     IGKNFDGLIDAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(12075..12371)
                     /gene="fis"
                     /locus_tag="OO161_001997"
     CDS             complement(12075..12371)
                     /gene="fis"
                     /locus_tag="OO161_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5463067.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12397..13362)
                     /gene="dusB"
                     /locus_tag="OO161_001998"
     CDS             complement(12397..13362)
                     /gene="dusB"
                     /locus_tag="OO161_001998"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5463068.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13691..14572)
                     /gene="prmA"
                     /locus_tag="OO161_001999"
     CDS             complement(13691..14572)
                     /gene="prmA"
                     /locus_tag="OO161_001999"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5463069.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14584..16035)
                     /gene="panF"
                     /locus_tag="OO161_002000"
     CDS             complement(14584..16035)
                     /gene="panF"
                     /locus_tag="OO161_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5463070.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(16025..16267)
                     /locus_tag="OO161_002001"
     CDS             complement(16025..16267)
                     /locus_tag="OO161_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5463071.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16360..17295)
                     /locus_tag="OO161_002002"
     CDS             complement(16360..17295)
                     /locus_tag="OO161_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5463072.1"
                     /translation="MGELLAEFLSRNPHQTFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            complement(17327..18157)
                     /locus_tag="OO161_002003"
     CDS             complement(17327..18157)
                     /locus_tag="OO161_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5463073.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            18500..19354
                     /locus_tag="OO161_002004"
     CDS             18500..19354
                     /locus_tag="OO161_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HCR5463074.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQSAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            19390..19566
                     /locus_tag="OO161_002005"
     CDS             19390..19566
                     /locus_tag="OO161_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter"
                     /protein_id="HCR5463075.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKTMSI"
BASE COUNT         5194 a         4893 c         4687 g         4798 t
ORIGIN      
        1 agcgcgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcaaattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggatcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc tcgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgttcca ctatctcccc acgatccata aaaattaccc ggtcagcgac ggtacgtgca
      601 aaccccattt catgtgttac acacaacatt gtcatacccg actgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgagcgccg acgttggttc atcaaacaac
      721 ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacct
      781 gaaatctgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc
      841 atcgccagag cttcagcctc tttcttaggc atcttgcgta cccaaatcgg tgccagggta
      901 cagttctgta aaacggtcag atgagggaag agattgaaat gctgaaatac cattcccact
      961 tcctgcctga cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctacgaca
     1021 atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg
     1081 gaaccagaag ggccacacag aacgatccgt tctcccggtt gcacggttaa atttatattt
     1141 ttcaaaacat ggaattgtcc ataccattta tggacgtttt ccagcgtaat catcgcgtta
     1201 gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggcgttaaa
     1261 acgtttttcc agatgttggc tataacgtga catgctgaaa caaaagatcc agtagatcag
     1321 cgcggcgaaa acataccctt ccgtcgacat acccaaccag gcgggatcaa cggttgcctg
     1381 ctgaacgcta ctgaaaagat cgaacaagcc gatgatgatc accaggctgg tatctttgaa
     1441 gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacacct gtggcagaat
     1501 aaccagcccc tgagttttcc agtaccccaa cgccagcgac tctgccgctt catattgccc
     1561 tttaggtagg gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag
     1621 gatcacgcca accagcgcgc ggatcagttt gtcgatactg gtgccttctg ccataaacaa
     1681 cggcagcatg accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc
     1741 gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag
     1801 taagatcccc cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt
     1861 cagcccgccc cattgccggg tttcaacccg ctcaagacca aaaaaaccgc catacatcag
     1921 ccaccagaca atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg
     1981 gagtattttg gtgcgattga cacaaggcca atcagtaatg ccaggttaat tcgccagcgt
     2041 tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat gaagacccaa
     2101 caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccagttagc ctgcaaaaat
     2161 gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc aatagtcagc
     2221 aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc gcggcttgag
     2281 ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat gactccttag
     2341 cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat agtcaggctg
     2401 ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc cgtctggttc
     2461 agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc ggcaagagag
     2521 gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac tcgcagtgcc
     2581 tggggaataa tgacctggcg tagcgtaacc ccagtgaccg agccgcttca tgttgaccat
     2641 aaggcactgc ctggctccca gcgcggataa tctcggcgat aaatgcagag gtataaaccg
     2701 aaagtgccag cgttaaggct gccagttcag gaattaaaac cattccgccg cggaagttaa
     2761 agcctcgtag ggctgggaca tcccagtgta gtgctgcgcc aaaaagccat tgcgccagta
     2821 aaggcaaacc gatgatcaac actgcggcga acggccaggt gcggcgcagt tgtctggtct
     2881 ttatctggta tgttttatta aagcggaata gcccaacaga aaggactata gccataacaa
     2941 cagccaggat aaacgcaata aatccgtctc ccagctgcgg tgacggaata taaagcccac
     3001 gattgctcaa aaaggccaga tcaaacgcgc tcacagcctg gcgcgtggtc cgggcaaatt
     3061 gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc gggggaatat tacggaagat
     3121 ctcaatataa attgtggata gctttcgtag cagccagtta tccgaaagtc tcgccagacc
     3181 gataaagaag cctaaaacag aagcgaacac aatacacaat gcagaaacca gtagcgtatt
     3241 gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc tgctggtaat cgatcaaatg
     3301 ctggacaata ccgaagccag cgccgcgatc cagaaaggca aaacctgaag taatgccacg
     3361 attactgaga ttcgttacag tgttgtgaaa taaccaacca acaattccga caacagcaac
     3421 aacggcaagg atctggaata accaggcacg aaccgtaggg ttggcaaagg agagtgagcc
     3481 tttaacggtt gagcggcgat gagacataga aacctcggaa agcataacgc tgaagagagc
     3541 gccgccgtag cgacgctcgt tacctcacac cgacttaacg cacgggcggc gcgtactgaa
     3601 taccgccgtt attccagaga ttattttgcc cacgtttaat tttcagcggg ctttctgaac
     3661 ctacgttacg ctcgaaaatt tccgagtagt tacccacctg tttgatgatg ttataggccc
     3721 atttattatc caacttcaga tccttgccgt aatcgccttc tttacccagc agatgtgcca
     3781 tatcaggcgt tgctggatta gccgcttttt catcgacgtt ctgggaattg atgcccatct
     3841 cttcagcatt cagcatggcg aaaagcgtcc agcgtacaat cgagaaccat tcatcatcgc
     3901 cacgacgaac taccggccca agcggttctt tagagataac ctccggtaag acaatccatt
     3961 cagctgggtt gcttaatttg atgcgcaggg catacagttg cgactgatcc gaggccagcg
     4021 tatcgcagcg accagattcc agtgccttcg ctgattcgtc agagcgatcg aaagtcaccg
     4081 gtgtgtactt catattgttt gccttgaaat agtcggcaac gttgagttcg gtatcagtac
     4141 ccgcctgaat acagacggta gcgccatcca gttctttcgc gctttttagc cccgctttat
     4201 cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc agtaaatgcc attcccatcc
     4261 cggcgtcgcg agatgaagtc caggtcgtat tacgggagag caaatccact tcccctgact
     4321 gtaaaaccgt gaagcgttct tttgctgtga gtggggtata tttcactttc gtgtcgtcac
     4381 caaatacagc agcggcaaca ctacgacaaa catcaacatc aatacctgaa aacttaccgt
     4441 ctgcatcggc ataagagaac ccaggtaatc catcactgat cccgcattgc acaaaacctt
     4501 ttttctgaac ggcatcaagc gtcgcgccag catgcgcctg atttgcaacg gcaagcagca
     4561 cgctggcggc agccagtgtg gctatcatca tctttttcat aatgcttcct gtgaggcgaa
     4621 aattatcgtt ataggacgtt tgggaaacgt cttttacctg tctgtggcca tcctcttata
     4681 agcaaaagta gtgccagctt ttgcgcacga tttcgcgtcg caataagctg agtgtagaca
     4741 ggaaataata aaaacagcgc cccgcaatgg tgcgaaaatg aaacatgcgc cacattttgg
     4801 cgcaaaatat ttacgcgtgt tttactaagc agaataatga ttgttagcgt gagtatattt
     4861 ctggtgggaa gtgtgaaagt taaattgatg cgttatttcg aaagcagcga aataacgcga
     4921 tataacatgc taaatcagca gagaattagc cagcagaata tagcaatatt ttatcttatg
     4981 taaagatact gtatcgttaa acagagcgcg aaactccgcg ccctgttcag ttatttcgac
     5041 agagcgatga tccccattgt caggccagca acagccgctg ccccgccggc aatagcccca
     5101 gccgtttccc agttatcctg agtcgtgcct ttcatacagg tattggtgtg aaccagacga
     5161 ccctgatcgt cataaaccgg aacgcagggc gaatcgtgag cacagcccgc cagcaatgcg
     5221 gtgagcaatg ccacaggaat taatcttttc atggtgttac ctcagtaata aacgctaacg
     5281 cttaataagt taactctctt attaacgagc tgaatgccat tttatcaccg gaggttataa
     5341 atattgcgga atgaataatg acaaattatg tgaattgtaa aaatagtgaa gggagcggga
     5401 actatagaaa gtttttaaag agtgagtgga agaatatctt attaattttt atgggtttca
     5461 ctgaaaataa taaaggcacc cgaaagtgcc tttatgtttc tgatttatcc tttaaagcaa
     5521 cggcggatca ccacaaagaa caccggtacg aagaagattg ccagcaacgt tgcagagacc
     5581 attccgccca ttaccccaat acccactgcg ttctgcgcgc cactgcctgc accgttactg
     5641 atagctagcg gtaatacgcc gagaataaag gcgagagagg tcatcaggat aggacgcaga
     5701 cgcatacgta ctgccattag tgtcgcttca acaacacctt taccctcttt ctccatgaga
     5761 tctttagcga actcaacaat caaaatagcg tttttggccg acaagccaat tgtcgttagc
     5821 aagcccacca taaagtagac gtcatttttt tgattaaaga gtgttgccgc cagcagcacg
     5881 tcgacaatcc ctaacggcac gaccaacata accgagacag gaattgacca gctttcatag
     5941 agtgcagcaa ggcacaggaa aacaaccaca aaggaaattg ctaccagagc gggagcctgg
     6001 tttcccgata agcgttcctg atacgacata cccgtccagt cataaccaat gcccgcaggt
     6061 aattttgacg caaggttttc catcaacgcc atggcatcgc cggaactggt tcctggcgcg
     6121 gcttccccct gaatctccat tgacggcaga ccgttgtagc gttccagtcg cggagagcca
     6181 tacacccaat gtgaagtggt aaaggccgag aatggcacca tttcgccgtt ggcgctgcgg
     6241 acataaagtt tatcgacatc ttctggcagc atacggaatt tggcatccgc ctgaacatac
     6301 acctttttca cgcgaccacg gtcgatgaag tcgttaacat aagtcccacc caacgccgtt
     6361 gaaatggtct gattgatgtc agaaagtgaa acacttaatg cctgcgcctt ttcctggtca
     6421 acttccagtt taaactgcgc ggtgtcttcc aggccattag gacgcacgct gactaagctg
     6481 gcaggatgtt gcgccgccat accaagcaac tggttacggg cctgggttag ggcatcgtga
     6541 cccagcccag cctgatcaat taactcaaag tcgaaacccg ttgccgtgcc cagttcaaca
     6601 atggctggca tattgaatgg aatgacaaaa ccgtcgcgga tcttgcccaa ttccattttg
     6661 gcacgatgga ttaccgcttc cgcactgttt tcatcaccat tacgctcttc ccacggtttc
     6721 agactgacga aggccatacc ggcgttttat gcctggccgc tgaagctaaa gccgttaacc
     6781 gtaaagacac tttcaacgtt cgctttctcg ttcttcagat agtaatccgt cacttgatcc
     6841 aacacttttt gcgtccgctc ttgcgtcgcg ccagcgggta actgaatcat ggtcagaaag
     6901 acaccctgat cctcttcagg taagaaggaa gacggaagac gtaaaaacaa caccaccatt
     6961 cctgcaacaa tcagcgcata gatcagtaaa tatcgtcctg tggaaccgag gattttgccg
     7021 acgctgttgg tgtagtggtt aacgctatga tcgaaggtgg tattaaacca gccgaagaaa
     7081 ccgcccttat tttcgtgatg ctcagcagag acgggtttaa gcagcgttgc acataacgca
     7141 ggggtaagaa tcaatgtcac cagaacagaa agtgccattg ccgaaacgat ggtgatagag
     7201 aactggcgat aaattgcccc agtagaaccg ccgaagaatg ccatcggaat aaataccgct
     7261 gacagcacca tcgcgatacc caccagtgcg ccctgaattt gcgacatcga tttttccgtc
     7321 gcttcttttg gcgggagctt atcctccatc atcacgcgct cgacgttctc caccaccact
     7381 atcgcatcat cgacgagcag cccgatggca agcaccatcc cgaacatcgt tagtgtgttg
     7441 atggagtaac caaaagcggc gaggatggca aacgtcccta acaacaccac gggtaccgca
     7501 atggtgggga tcagcgttgc tcgcatattc tgcaagaaca gatacatcac caggaacacc
     7561 agcataatgg cttcgaacag cgtttttacc acttcgtgaa tagaaagctg gacgaatggc
     7621 gtggtgtcat aagggtagag aaccttcatt ccctgcggga agaatggctg taattccgcc
     7681 agttttgcct taatggcttt cgcggtatcg agagcattcg cgccggttgc cagcttaatc
     7741 cccaggcccg ccgccggttt tccgttgata cgagcgataa cgttatagtt ttcaccgcca
     7801 agttcaaccc gtgcgacatc tttcaggcgt accaccgagc cgtcactgtt tacgcgcagg
     7861 gtcactttgc cgaattcttc cggatttttt aaccgcgttt gagcaataat cgaggcattc
     7921 aactgttgcc ctggtaacgc tggcgttccg cccaactgtc cggcagcgat ctgatcgttc
     7981 tgtaccttca actggttaat cacatcaacc ggtgtcagtt tatatttgtt tagcagatcg
     8041 gcgtccagcc agatacgcat cgcatactgt gcgccgaaaa gctgtacgtc accgacgcca
     8101 ttcagacggc taagcgtatc tttaacgtta gaggccacat agtccgagat atcgtcctgt
     8161 gtggtgcctg ggttatcaga gacaaaaccc gccaccatca aatagctgct actggacttt
     8221 tcaacactga tcccctgctg ctgaacctcc tgcggcagca acggcgtggc gagctggagt
     8281 ttgttctgca cctgcacttg cgcgatatca ggatcggtcc cggactggaa ggtaagggta
     8341 attgtcacgc taccggcgga atcgctggtg gaggacatat acatcaggtt atcgatacca
     8401 ttcatattct gttcgataac ctgcgtcacc gtatcctgca cggtctgcgc atcagcacct
     8461 ggatagtttg ccgaaacaga aaccgcaggc ggtgcaatgg ttggatactg agcgacgggc
     8521 aattgtagga tcgccagtgc gcccgccatc atcagaataa tggccagcac ccatgcaaat
     8581 atcggtcgtc gaataaaaaa gtttgccatg tcagattacc ttacttcgat gcagtatctg
     8641 cgggggtatc ggtagtggct ttaacctgct ctcccggacg cgctttttgc aggccgctga
     8701 caatgacttg atcgccagat ttcagacctt cgctaatcaa ccatttatcg ccaatcgcct
     8761 gactggcaac gacagggcgc gcttccacct gacttttatc gttaacaatc agcacggttg
     8821 catcaccacg cggtgtgcgg ctaacgcctt gttgcgggat aagaatggcg tcaggttgga
     8881 cgccttcatc aatccgtgca cgcacataca tacccggcaa aagcgtatgt tgcgggttag
     8941 ggaagacagc acgtagggtt atggagccgg tgctttcatc aacggtcaca tcagagaatt
     9001 gcagcgtacc tttcagggga taggtttgac cgttttccat gaccaactct acgttgctgg
     9061 tggcgttttc cttatgcaaa tttccttgct ctacggattg cttcagcctc ataaaatcgt
     9121 tgctggattg ggtcacatca acgtagatag gatcgagctg ctggacagtc gccagttcag
     9181 tcgtttgccc attagtgaca agagcgcctt cggtcacggt cgatttgccg atacgtccgc
     9241 taattggcgc agtcactttg gtataagcga gattgatgcg agcgctttcg actgtggctt
     9301 ttgcggcaat cacggcggca tcggcctggc gagcatcagc aatggcctgg tcgtactcct
     9361 gctggctgat gtatttcgta cccacgagcg gaacgtaacg ttttaccgtc aaatgcgcga
     9421 tggcggcggc ggcttcactt ttcgccagtt cgcctttcgc gctgtcataa tttgcctgat
     9481 aggtcgcggg atcgatctgg tacagggact ggcctgcttg tacatcgctg ccttcagtga
     9541 aattacgatt cagtacgatc ccgctaacct gtgggcgaac ttcggctata cggtaagcat
     9601 tggtgcggcc tggtaattca gtcttaactt ctaacggggc cgttttcaca atatgaacgg
     9661 taacctgcgg ttcaccgacg taagctttct cttctccctt atcgttacaa ccggcgatta
     9721 aagccgcgga gatcagaata aaggagggca ggaggaaaaa cctggcatgt ttcgtcatta
     9781 ctattcctca aaaaaccaaa agcgcgttat ttacccagaa aggcaacgcg ttaactcgca
     9841 gaaagaaaaa tacagtttgc tatcctacaa attatcattc gtcgatgtaa ggaatagtta
     9901 tgaatactgg cgtctcaagg cacataaaca caaaaaaaga ttaatattct actgttttat
     9961 tttgacgcgg gttgaaagag gcagaattaa aacctcgtaa attgaaatat atattcatgt
    10021 ggcgaatgta ttttaggtaa ataatataca ttgtttattt gacgatttat agcaactcaa
    10081 tgttaccctc tcgtataaat acacattaga tgatagatta accttcgcta ttttctcacc
    10141 ctgtgtcgaa tatatttatt tcctgaataa ttaatcatgg caaaaagaac caaagccgaa
    10201 gctctgaaga cccggcaaga actgattgaa actgccatcg cccagtttgc gcagcatggc
    10261 gtaagcaaga cgacgctcaa cgacattgcc gacgccgcta acgttacgcg tggcgctatc
    10321 gactggcact tcgaaaacaa gactcaactg tttaatgaga tgtggttgca acagccttca
    10381 ttgcgggagt taatccagga acacttgacg gctggattag agcatgaccc gtttcaacaa
    10441 ttgcgtgaaa aattgattgt cggcttgcaa tatattgcca aaattccccg ccagcaggcg
    10501 ttgctgaaaa tcttatatca caaatgtgaa tttaatgatg agatgctggc cgagggagtg
    10561 atacgcgaaa agatgggctt taatccgcag actctccgcg aagtattgca ggcgtgtcag
    10621 caacaaggtt gtgtagcaaa taacctcgat ttagatgttg tgatgattat tattaatggt
    10681 gccttcagcg gaattgttca aaactggtta atgaatatgg cgggttatga tctttataaa
    10741 caagcacccg cgctggtcga taacgtatta agaatgttca tgccagatga aaacataacg
    10801 aaattaattc atcaaacgaa tgaattaagt gtcatgtaaa ttcacattct gtcctgaaaa
    10861 ttcagttcgt cacagttaac cagttttagt ggattaactg agttcatatt acggcatttg
    10921 tctgtttctg ttgtcagcag ttcaatccac tgcatcagta aagtatcaaa cagaaaaacg
    10981 aagacagcga aaacgacgag ccaccaatac ttgcgaatca ttacctgagc ctgtgcatgt
    11041 ttaaccaagt ctggcaggaa atatacacga aatgaataat ctgaaaacca ggcttacact
    11101 tcctttactt tgcaatgaga tcggggtcaa cttcgcttaa ccggcttcgt tttgacaggt
    11161 tgccggtctt tctttttttc actttcgcca gttcttccgc agagtaatgc gacgcgacat
    11221 ccagccgtcg aagccccatt ttgatgtact cgctgttgat ctcaatacca atgaattttc
    11281 gcccgctgga gacggctacg gcaccggtag taaagctacc ggcaaacggg tcgagaacga
    11341 tatcgcctgg gttggaagag gcgagaataa tgcgtttcag taaggcttcc ggtttttgcg
    11401 tcgggtggtt ctcatattca tccatcaaat aacgcacgcg cggaaaatcc cagacgttac
    11461 ccggtacttt ttgatgattg tatggctgtg gaggattttt gcgataatcg atcaacgcgc
    11521 gctgcgatcc ggttttggct tctaccagaa tagcatcacc gttgaatgtg tagttctttg
    11581 cgtctttcac catcatcagg atggggtcgt acatggagcc gtagtgtttt ttcgcctgta
    11641 caccagaact gtcatatgac cagacgatgc gacttttgat ggtaaaaagc ttgcggcact
    11701 ggagatcgat aaagggcatg ttttccgtgc tgttcatgat gtacatgctg ccctgctttt
    11761 tcagaacgcg gtggcactct gcaatcactt caaacagcca gtcgataaac agatcttctt
    11821 tccaggcgtc gatcagacca tcaaaatttt taccgatgtt atatggtggg tcggcaagga
    11881 tcagatcgac actttcggcg gggatctttt taagttcggc aagcgcatca ccgtgaataa
    11941 tggtcttagc ttcattacca aaccgggtcg gttcacatcc tgttctcatg gtcactccct
    12001 ttgtgacacc tataaaaaag gcgcttcccc atgccgagta gcgccttttt aatcaagcat
    12061 ttagctaacc tgaattagtt catgccgtat tttttcaatt ttttacgcag cgtaccacgg
    12121 ttgatgccca tcatcagcgc agcacgggtc tggttaccac gggtgtattg catcaccatg
    12181 tccaacaggg gctgttctac ttcagccagt accagctcat agaggtcatt cacatcctga
    12241 ccattcagtt gagcaaaata gttcttcagt gcctgtttaa ccgagtcacg caggggtttt
    12301 tgggttacct gatcctgaga gttaacggta gaaacggtca gtacgtcaga atttacgcgt
    12361 tgttcgaaca tagttctgtc agctctttat ttctgtttac gcaaaatttt cgaagtatgc
    12421 ctccaacgcc tccagctgtt cgctagcatc ctcaatggcg ttgaatgtgc gccgaaactg
    12481 gtcatttgga gcgtgttcct ggagatacca ggaaacgtgt ttacgtgcaa ttcggtaccc
    12541 ttttgccgga ccataaaagt catgcagttc ccgaacgtgc gcgcaaagca agcgcttaac
    12601 ctctgccaaa ggcagtgggg gcagcaactc cccagtgtcc agataatgct ggatttcccg
    12661 aaagatccag ggtcttccct gagctgcgcg gcctatcatc agggcatccg cccctgtata
    12721 gtcgagcaca gctctggctt taagcgggtc agtaatgtcg ccattcgcga taaccggaat
    12781 ggaaactttc tgcttaactg cccgaatact gtcgtactca gcttctccat tgaacaaaca
    12841 ggcgcgtgta cggccatgaa tggtcagagc ctgaatgcca cagtcttcag ccagttgggc
    12901 aatctcttcg cagttacggt gttccggtgc ccagccagtg cgaatcttca gggtaacagg
    12961 aacgtccact gcattgacga cctcggtaag gatcgattta acgacatccg ggtactgcaa
    13021 gagggctgaa cctgcgagct tgcgattcac ttttttagcc gggcaaccca tattgatatc
    13081 aataatctgg gcaccgcttt ccacgttaat acgtgctgca tctgccattt ctttcggatc
    13141 gctaccagca atttgcacgg tgcgaatacc gggttcatca atgtgcacca tccgtaaacg
    13201 agatttgtcg ctttcccaaa cctgtgggtt agaagacatc atctcggata ctgtcaatcc
    13261 ggctcccatc tcgtagcaca acgtccgaaa aggtctgtct gtaatgccag ccatgggcgc
    13321 tgcgatcagg cgatttctga gctgatattg tccgatgcgc atgagttaag aaatgaccat
    13381 actgtgactg caaggcggcg tatattacgc attttttgca cgagatgaaa ggccaaactt
    13441 tgaccaatcc tctgagatgg atcaaagaat tgcatttaaa atgagcgtgg tgcgataatt
    13501 acttataaaa atcatcatat tagaaaatag tgactaaaat ttacattcaa agaaatttga
    13561 gtaagttctc aatttttctt tatgaatgaa aattttggca cgcaaatttg cgtaaataac
    13621 cggcaaattt acgtgccttt tgtgagcttg ctcgcacttc gccccgcgtc accctacggc
    13681 gatgcgaagg ttaattctta cgaccggtaa tacggcacca ctcttctttt tccacgaccg
    13741 ggtccagtgc gaagctatcg gcataagctt cacaaacgct ctctgcctgg cttgccagaa
    13801 taccggaaag gcccagcaaa ccgcctgaaa ccggcaggac gctgattaac ggtgccagtt
    13861 cacgtaatgg gcctgcaagg atgttcgcga ccaccacgtc ggctttcatt tcttctggct
    13921 gatctttcgg taagtagagt tccagacggt cagaaacgcc attacgttcg gcgttgtcgc
    13981 ggctagcctg aatcgcctgc ggatcgatat caataccaat ggcttttgct gcacccagtt
    14041 tcagcgccgc gatagccaga atgccggaac cgcagccaaa gtcgatgact gttttaccag
    14101 ttaaatcgag gctgtcgagc cattgtaggc acagagaggt agttggatgg gtacccgtac
    14161 caaacgccag ccctgggtct aacatcacgt tgacggcgtt ttcgtccggc acatcacgcc
    14221 agcttgggca gatccacagt cgttcaccaa agcgcatcgg gtggaaatta tccatccatt
    14281 cgcgctccca gtctttatct tccagttgtt cgattttatg cgcgaagcct gcgccgagca
    14341 gcggatggtt ttccagaatc gccaccacgt cgttcatatc ggtttcagcg tcgaacagac
    14401 caatcacatc ggtgtcgccc cacaggcgcg tttcgcccgg cagcggctca aatactggcg
    14461 tatcgtgggt atcctgaaaa gtgatagaaa cggcacccgc ttccatcagc gcatcgctaa
    14521 gatcttcagc gttcgcgccg gtggtgttca gtttcagttg gatccaaggc atggcaaaac
    14581 tctttattta tcagtagtca aaacggtagc ttgcgggacg gatgtaccga aacggtttcc
    14641 gaccaggaaa gccagcaaac ttagtagtaa cgagggcacg atagggtgga agcccaggta
    14701 ctgaatattc agcgtcgcga gtacggcata cagcacgccg ccaacgatca tcgcacttag
    14761 cgcgcctttg gcgttggcgc gttcccagta aagacccagc accagcggcc acaggaaaac
    14821 ggcttccaac ccaccgaagg ccagcaaatt cagccagatg atcatttctg gcggcttcca
    14881 ggcggcaagc agcagcaacg cgccgagaac taacgtaatt accgccgaca tccgcttcag
    14941 acgcgtctcg ttttgcattt gatccggacg gatattcaga tagagatctt taatgatcgt
    15001 agcggaactt tgtagcaatt gggcgttaat tgtcgacatg atcgcagcca taggtgcagc
    15061 cagaaagatc ccggcagcaa acggtggcag cacttttacc attaacgtcg ggatcaccag
    15121 gtccggtacg gtgagatcgg ggatcactgc acgacctaac gctccggcca ggtgcatacc
    15181 gaacatcagg attgcgacta caatcgtacc gatgatgatc ccccggtgta ccgctttgct
    15241 gtctttataa gagatacagc gcaccgcagt atgcggcagg ccaatcacgc caaagcacac
    15301 cagtacccag aacgacgtca taaaggcagg cgacagaata tcgtcagcgc cttgtggcgt
    15361 aaccagttgc ggatcgatgg tttgcagcgt ctctaccgca ttacttaaac cacctgcggc
    15421 atgtaccacg ccaataagca gcacaacggt gccaatcagc atcacaagcc cttgcatggt
    15481 gtcgttcagc acgctggcgc gaaagccgcc aaaggcggta tacaacgcaa tgctgatacc
    15541 gaaaatcagc agcccggttt cataaggaat acccgccgcg gtttccagca ggcgcgcacc
    15601 gccgataaac tgcacggtca ttgccccaac gaacgcaacc agcaaactca aactcgccag
    15661 ccacaccaga agacgactct ggtagcgggc aaacagcatg tcgttcagcg tcactgcatt
    15721 gtagcggcgc gcaagaatcg cgaacttctt gccgagaata ccgagcgaaa gccagactgc
    15781 aggaagctga atcatcgcca gcaataccca acccaggccg tatttataag cagctccagg
    15841 cccgccgata aacgaacttg cactgatata ggtcgcggtg agcgtcatcg ccagcacaat
    15901 accgcccata gagcggctgc cgaggaaata ctcattaagg aaggtaccgg tgctccgttt
    15961 acgcatcgca taaacagaga taccgaacac caccaccaga taggcgacca gcggtagaat
    16021 cacttcaagc tgcatcgtca tcctccagtg ggatatcgcg atagataaat ttcaccatcg
    16081 cccagcacag tccaataaac agcagcggcg tcaggatgca ggccatctca aaccagcgcg
    16141 gaaagccggt aaaaccgggg gcaacgtcag gtaagtaagc ggctgctaac caaactgcca
    16201 gatacaaaag ggtcagcccc agcgcccagc gcgcctcttt atgggcctga acaaaacgag
    16261 tgtccacgga agggttcctc ctcatggggg gatacgagaa acgggcaatt ctacggcaac
    16321 tcttcaggca gggaaaataa tctgacagaa aattgccgtt cagtgctgct gcaaaaagtc
    16381 ttcaatgtca gccagtgagg atatcccttc catcggacct tgccgcatta cggcgagcgc
    16441 gccgctggca tttgcatatt gcagtgcctt atgtgccggg aaaccggata agaaaagagt
    16501 gataaacgtt gcgccaaagc agtcgcctgc acccgttgga tcgataattt cgatatcgtg
    16561 gcctgtaaca tgctgggcgt gtaatgtacc gtttttaagc ttgtaatagc tggccccacg
    16621 ctgggcgcgt tttatcgcca catgttttac gccgccgtgg agaagatcgc taacaatctg
    16681 ttcttctgac agatttttgt gacgcgcgaa gaaagggagt tcgctttcgc tggggatgaa
    16741 aatatccgta tattcaatca aataatcgag agcctgcgcc atttcaggaa tgctcagcat
    16801 ctctttgcga atattgggat cgaaagaaac ggtgccgcca gccgatttga tcgtcgttat
    16861 tgctttacgc atgacatcga tcatgcgaaa tgagaacaac gatgagccca caatatggag
    16921 atggttacac tgttttagca gatccttatc aatgtgctca gcagtaaaca agccgcaggc
    16981 gctgttaggg atattaaaga cgaaatcgcg ctgctgggga ttttgataac tcacgaacgc
    17041 cgtacctgtg acggcattgt tcataacatg gatcccatcg gtaatgacac cttcatgacg
    17101 gaggcgctct ataatgagtc ggccaaaatc atcattacca acacatccga agaagatggc
    17161 ccgatgggac agttttgcca cctgagcagc aaaaattgct ggcgcaccgc tgggaaatgg
    17221 cccgataaac tccccaggct gagtgaatgt ttgatgtggg ttgcgggaca aaaactcggc
    17281 cagcaattcg cccatagcgc agatagttat tgtcacaaaa cccctctcat tcctgaagcc
    17341 tttcgcgatt ggaccaggta aagcactcat tactggtata aaggataacg aaatcagccg
    17401 actgcaattg ttgctgatat tgttcgttaa tgctgcggct gacgatcaca ttttctgcct
    17461 gatgcaaatg cccgatagca gccgttgatg gcgcaggatc ggcggcaacg ataaccgtct
    17521 tattctgggc atgatcgaaa cagagctgcc aggcagcggc cagtctggga acgttgatat
    17581 ttaaagtacc atcagcatcc agatggttga tttccagaat gctatgtgta atggtgaaat
    17641 gctgaagtgc ctgttcaagc ggttttccgg ccagtgcggt gtcggggcgc agcatttcac
    17701 tgccagccag tattatttcg ccatcaataa actgatttgc cagcagcgca tgttcgggat
    17761 cgttggtgat cagacgcagt tttttcactt cgctgagcaa tggaatgatc ttacggcaga
    17821 ttgtcccatg tccaagaaat agcgtgtcac gatcgcgaat tagccgtgcg caatgtcgtg
    17881 ctatttccat ttgttgtgcc agagacaatg gcgcgatcgg ttgtatcgct tctggttcag
    17941 aggagagtgg tgtcgcaata gcaccgccaa atgatcgctt taaatatccc tgttgctcca
    18001 gataactgag atcgccgcgg atcgtcacgc cagagacacc aagttgtgca gataagtcat
    18061 caactttgat ttcaccttgg gcggcaacaa tttctgcaat ttttaatctg cgtttcagta
    18121 ggttagacga ttttctcggc acgttaacac tcttcattgg cctttaatgc gtaacactat
    18181 agccccttgt atttttcttt gaaagttatc cttcaataat atcgacaatt cgaaaagaaa
    18241 atcatgggtc aaaaatcgca acaaaaatag attaattaat tgaaaaataa atgaatttat
    18301 tcatatttat gcagagatgt tattggtttc acttgtaaag tgaaaaacaa attaagttat
    18361 ttttttgtaa gttattttga tgatattcga catgtatcac gctttttgcc gcttttgctg
    18421 tgctagtttg ctttcaaaat gaaagttaat attctctttc ttcattttgt aaggcgaacg
    18481 acaaataaca ggagctgcga tgtacatcat ttcaagcaaa aacatgctga aaaaagccca
    18541 gtcagaggga tatgccgtcc ccgctttcaa cattcataac cttgaaactt tacaggtagt
    18601 tgttgagagc gctgcacaac ttcgctctcc cgttatgctg gcgggaacac ccggtactta
    18661 tcgttatggt ggcgtgggaa gtttaatttc gttagtacaa tcgctggctc gcgagtacaa
    18721 cctgccgtta gtcctgcatc tcgaccatca tgaagagagt gatgacattt ttaacaaggt
    18781 tcgcgcaggg atccgttctg tcatgattga tggctcccat ttgccatttg aagacaacat
    18841 tgcgcgagtg caagaagtta ccaccttttg ccatcgcttt gatgttagcg ttgaggccga
    18901 gctagggcgt cttggcggcc aggaggacga tctgctggtg gatgaaaaag attccgctta
    18961 tacctcacca gccgcagctg cagagtttgt tcaacgtacc caaattgact ccctggccgt
    19021 agcaatcggc acagcacatg gtttatatgc gcatgaacca cgactggatt ttgtgcggct
    19081 ggaacagatt cgtcagcgag tggagattcc actggtgttg catggtgctt cgggcttatc
    19141 agaacaggac gttcagcact gtattcgccg cggtatttgt aaagtgaatg tcgcgacaga
    19201 attgaaaatt gcgtttgccg atgcgttaaa aggatatttc tacaacaatc ctgcggcaaa
    19261 tgatccacgc cactatatgc agtccgcaaa ggccgccatg aaagaagtcg taactcgtat
    19321 tattggtgtc tgtggtagtg aaggaaaaat ttaagacgtt gtaccctaca aaaacaatga
    19381 ggattgttta tgaaaaatat taaattactt ggttcagcta tgttggtgag cctggcattg
    19441 ttctcgcaaa attcgttggc aaaagagtat aaaattggtg cgtcattatt aacacagcaa
    19501 catcctttct atattgatct tgcgaatgcc atgaaagaag aggcaaaaaa gacaatgtca
    19561 atttaagtgt ct
//
LOCUS       DAJTNA010000067        19192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTNA010000067 DAJTNA010000000
VERSION     DAJTNA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19192
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.068"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            533..685
                     /locus_tag="OO161_002006"
     CDS             533..685
                     /locus_tag="OO161_002006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463076.1"
                     /translation="MVDNIDVMKNVEMLIGYRAIKSKYLSILQNFGAGNIHLDNANRV
                     WELRRI"
     gene            828..1049
                     /locus_tag="OO161_002007"
     CDS             828..1049
                     /locus_tag="OO161_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463077.1"
                     /translation="MRLALREDVCDWFMAKGETRSLLENVHAVNEKNKAPSISTEVIT
                     VLLMWKRLTFDSQKRGGDLRPFLEKWNHT"
     gene            1186..2079
                     /gene="galF"
                     /locus_tag="OO161_002008"
     CDS             1186..2079
                     /gene="galF"
                     /locus_tag="OO161_002008"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5463078.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEALGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            2390..3709
                     /locus_tag="OO161_002009"
     CDS             2390..3709
                     /locus_tag="OO161_002009"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HCR5463079.1"
                     /translation="MVIKNIFWQFAEKILRLIFAIFISALLARHLGVSGFGLYNYINA
                     IISVALVVTSLGFNRILVRESTCCKDKISHNELISTSFYSRLLFSILIFALLWGGYSY
                     IDNKNSIIYLISFLSVIFVSFDVIDYHLQGQCIFKTATLCRVVSFLIVSFIRIFLVFE
                     NATLIWFCITILFEYAISAILLYLSSRFIHRNIFTIKIKHVSLNKAKYLLKESWSEII
                     AGFGAILFMKMDQIMLQYMLNAESVGVYSAALRISESWYFLPAAIVSSVFPVIMNKYN
                     KENTNESALVELFGHIMTLLVWLSICAAITISLFGPAVIHWLFGDEYIRSATILIYHV
                     WAGVFLSMGILSGSWLVAKKKLKLNLYRNIGGLIVNFFLNIILIPVYSGVGAAIATIS
                     GFAFAFLFFDLFHKELRGMFFLKIKSLSPINLIQAIRFLSNIRLKYL"
     gene            3926..4184
                     /locus_tag="OO161_002010"
                     /pseudo
     CDS             3926..4184
                     /locus_tag="OO161_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            4458..6815
                     /locus_tag="OO161_002011"
     CDS             4458..6815
                     /locus_tag="OO161_002011"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:NF016145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HCR5463080.1"
                     /translation="MNMKNIIERIKPLFFDGSRHLVRPVVLQFPVIDICNSKCQMCRI
                     WENKKSVDISVEQLKKGIQNELFKDVMSIGFNGGEPTLREDLSEVIETCLYSLPKLKQ
                     ISLITNGFKYDQVIHQIEKIGKLIKSKDIFFDVMVSLDGFGVIHDKVRGRTGSFKHAQ
                     HVLTFLKNADYVSNIRIGCTVIKENVYYLHELLEFCIREKLYVKYRQGVPHKRLYTEN
                     LIEPYSLTFEEKYEFVEFLEGIIKYYEPSLLQKHFYRSLINQIIYEHPRTAGCDWRYR
                     GATITAKGELAYCAIESKTLMKDISDGNAYQIYFDNENHLNDIKENKCKNCHHDYVGL
                     PPPAEYRKIFLSSINEKWKIKDKIKKIPGGRGINKIINEYKYSTCINQYRNIHSTQVQ
                     DVSLRGNTRVLICGWYGTETLGDKAIIGGIINSVRSSLGYEVVFYIASLYPYITEMTR
                     RQMPELKNCQIVNIQQAILLAKDMTYVVFGGGPLMAIDDIAPMQVIFEKAKQAKVKTG
                     IMGCGIGPLGDKRYNNSIKTILELSDFRIYRDQKSKSEALRLGVNAENDIVAEDPAFS
                     WIKSYYEQSVVGGRHRKAYINDEKEILLLGLRDFPWREYAKHLSEKECLSIKDNFEEV
                     LVSALKEVVKKRDNLIIRPLPMCTNHFGSDDRWFYRRLFRDSGIPSENLDFSLCGREL
                     APTEYCKAFLDANALLAMRFHSLVFGLGLGTPSLALDYTLGKGKVKSLAERFGANVIS
                     LSKINKEELLLEIEDLLEHNSQESNVSILESIKFQQSVDTFMKRD"
     gene            6817..8025
                     /locus_tag="OO161_002012"
     CDS             6817..8025
                     /locus_tag="OO161_002012"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5463081.1"
                     /translation="MKILHICYSDCLGGAARAANRLHQAQRKLGINSFMLVVDKYTDD
                     DYVLELNKLTKFRIKILNMISGYILRHLIQKNPVKHSLNIFSAGVVNAINKLNPDIVN
                     IHWIGDNMLSIKEISKIKSPIIWTMHDMWAFSGCEHYDSFPGIRRYEDDYNKKDGIFD
                     LNRIIYRYKKKKWSHINMQFVSPSRWLAECACRTSLIEGKRVHVIQNTIDHVVFDKVD
                     KVCARKLLGLPQNRKLILFGAMSGTADHRKGFHLLNEALKYLSEKYATEYGLVIFGDK
                     KDGDDIHGFPTYRLGTFYDELSIKLIYSAADVYVAPSLQDNLPNTLVESLAVGTPCIA
                     FNIGGMPDLIINSKLGGLVTEVDSRALSIAILDVLNRNIDSNIIRNISLAERAEDNIA
                     HKYEDVYLNA"
     gene            8018..9211
                     /locus_tag="OO161_002013"
     CDS             8018..9211
                     /locus_tag="OO161_002013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463082.1"
                     /translation="MHKKLYYYFLIVSPLIYLTGGIEHVTPLSIAGYLDLPSLSLINY
                     IFFLWCVFFLHFYSFGKNILWLYLLDIVIIMIIFRESRVDSIFFSTLSQFLILSPLFL
                     LVGCKIHIGYERIKKISYIFVFYLICSILLHYIFIMYYKYIGFSLPNERAVGIFKNPN
                     HLAAFSILLLIFLYNLAFIKLVDVKKIFFAELIVAVTVYLSGSRSSQVFFIILLLIHS
                     FLYNKRFFSLYCCLAVLSVAYVGMSDIGVDKIEQLLTKRETKDIAEAGNMRMSILFDM
                     LENFNFREILIGKGSGQGTAVYITNQSNNNEKITWLDSNVNTLTYTYGVVFLIFSLAA
                     MLLKMLFLALMKRTGEFYIFFFVFYFMWFINIGEFFPLIFMLLIQVSTSNMGQQHDIN
                     NYYKL"
     gene            9183..9944
                     /locus_tag="OO161_002014"
     CDS             9183..9944
                     /locus_tag="OO161_002014"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5463083.1"
                     /translation="MISIITINYNNKNELYRTLSSIDSQKLKSHIEVIVIDGGSTDGS
                     QEIVSNSFKHLVTYFISEPDMGIYDAMNKGILLSNKEWLYFLNAGDIFFSDDVLNKLY
                     DFIINSNLTNLIYAPYISDGKIDNTQLFSLSYLSSHMINHQSMLLHKELFQKQLYDIN
                     YRFCADYALLLNLFDNIKPVKTDFIIVDYDSKGISSLDDNKVFIWKERILAVLKSNLS
                     FYKKLFLLKRAFGVYPYHLLKSKLKCMKNIMTHCR"
     gene            9911..10750
                     /locus_tag="OO161_002015"
     CDS             9911..10750
                     /locus_tag="OO161_002015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463084.1"
                     /translation="MHEEYNDTLSIVFCALYDDWLKNKELIHRSFEHFVKIWEERCFF
                     ILIIQDAKQRKIELAFKNMHIFYMQERGISLARNRGVEEACTLNSRWIIFHDASICWT
                     EEAVGFLFKNRFSKISPKVKLKFQLIEEKPSTDVQGEIRKINPVYDTYVGSVLFEVQS
                     IAKIRFNIFHGPGQNTIYKSGEDVLFFFDYFSMKKNFCVYESKTAYIYHPPREKDFSK
                     HKIYAKGQGRVFKILLKEYFSIRLLMDCILFFGNALVRCIFFRKNSFYILKERCVGFF
                     CED"
     gene            10752..11870
                     /gene="gmd"
                     /locus_tag="OO161_002016"
     CDS             10752..11870
                     /gene="gmd"
                     /locus_tag="OO161_002016"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5463085.1"
                     /translation="MKKALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTDRVD
                     HIYQDRHNDSPNFLLHYGDLTDSSNLIRLIKEIQPDEVYNLGAQSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQETPQRETTPFYPRSPYAVAK
                     MYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYLG
                     NMDSLRDWGHAKDYVRMQWMMLQQDHPEDFVIATGKQISVREFVRMSAREVGLELEFS
                     GEGVDEIATVVNKTSDCAIGVNIGDVIVRVDPRYFRPAEVETLLGDPSKAKKVLGWEP
                     EITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            11874..12839
                     /locus_tag="OO161_002017"
     CDS             11874..12839
                     /locus_tag="OO161_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5463086.1"
                     /translation="MTKKRIYVAGHRGMVGSAICRQLSLRDDIELVVKTHKELDLTVQ
                     KDVEVFFEQERIDQVYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAEQPMKESALLTGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGMNDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDDVIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGFQGKIVFDSTK
                     PDGTPRKLMDVTRLKNLGWQYRYNLHEGLSLTYKWFLANINSFRG"
     gene            12842..13303
                     /locus_tag="OO161_002018"
     CDS             12842..13303
                     /locus_tag="OO161_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5463087.1"
                     /translation="MKKRLEYELFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
                     NYWFVPGGRIFKDESFEAAFKRITFEELGVQTSLNEALFLGVYEHFYNDNFSETEFST
                     HYVVHGYEIQLNPQQLHLPTIQHNSYKWFDIETLLNSTDVHQYTKNYFISR"
     gene            13309..14715
                     /locus_tag="OO161_002019"
     CDS             13309..14715
                     /locus_tag="OO161_002019"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HCR5463088.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPTLVICNESHRFIVAEQLRKEGLKHSGILLEPVGRNTAPAVALAALQAKASG
                     EDPILLVLAADHEIQDEGRFIAAVLAAKNFAEQGKLVTFGIVPAAPETGYGYIKVGEE
                     LDGQGYKVAAFVEKPELDLAKQYLADGGYLWNSGMFMFRASIFLDELKKFRPDILASC
                     QQSLSSSTQDLDFIRLDNSSFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLIEDATDSYLYSQHRLIGAVGVKDLVVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGQRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPIGIPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
     gene            14715..15461
                     /locus_tag="OO161_002020"
     CDS             14715..15461
                     /locus_tag="OO161_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5463089.1"
                     /translation="MRISIITATYNSEKTLLDTLLSLEKQTHPDIEYIVVDGASKDNT
                     IKLIKSNCTRVSKIICEPDNGIYDALNKGIQAASGDVIGFLHSDDLLAYDDVIADIAK
                     AFERSGCDAVYGDLEYVAQNDTTKRIRLWKSGAFSRLKMKLGWMPPHPSFYMKRDCYG
                     QFGCFSLDYRISADYDSLLRYMLKQRISITYLPKVLVKMRVGGISNRSVSSMVNKSME
                     DIRVMKHNGIIWPIALAYKNLSKLPQFIKK"
     gene            15467..16891
                     /locus_tag="OO161_002021"
     CDS             15467..16891
                     /locus_tag="OO161_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCR5463090.1"
                     /translation="MLNAKKIITGSNIAFGTSGARGLVVDFTHDVCAAFTHAFLSVIQ
                     NNFNFRIVALAIDNRPSSYEIAQACAYAIKQHGFEVEYHGVIPTPALAHYSMQKNIPC
                     IMVTGSHIPFDRNGLKFYRPDGEITKEDEFAIVNSEYTFSPVGVLPHLETSSQGADCY
                     LERYVSLFNSDILKGKRIGVYEHSSAGRDLYAPLFNQLGAEVISLGRNDEFVPIDTEA
                     VSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKA
                     LAIPVSCNTAIEQSNKFASIQRTKIGSPYVIAAFAALGKQFDSVAGFEANGGFLLASD
                     LQINGKELNSLPTRDAVLPALMLLIASRNSTISQLVNNLPQRFTWSDRVKNFPSESSQ
                     QIIKNALSAPNKFLNRLGYESLSCSAIDETDGARFTLTNGDIIHLRPSGNAPELRCYS
                     EASNENQAKQYVTKVLGNITSLIS"
     gene            17085..18491
                     /gene="gndA"
                     /locus_tag="OO161_002022"
     CDS             17085..18491
                     /gene="gndA"
                     /locus_tag="OO161_002022"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5463091.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLEKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKDAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPKIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            18732..>19192
                     /locus_tag="OO161_002023"
     CDS             18732..>19192
                     /locus_tag="OO161_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR5463092.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     VVDKEIQNFLQSDEIRFNATLDKKEAYRNADYVIIATPTDYDPKTNYFNTSSVESVIQ
                     DVIEINPFAVMVIKSTVPVGFTAAMQTKYHTENIIFSPEFLREGKALYDNL"
BASE COUNT         5910 a         3129 c         3974 g         6179 t
ORIGIN      
        1 agtaatgata aatgtcaccc ttcgatcatg gataaatcag ggccaaaatg caatgttaca
       61 gcaccattta tccgacatta tcctggagct tatcggcaca atatccatca tttgttgtac
      121 gtgagaaaat tataaatctg gttaacaaat gtcgtgtggg gagatgttgt attaaaagtc
      181 accagcctaa agaatgtact aaaaagcgtc gtgaaatacg cttcatataa ttcctgaaaa
      241 tcgctaatgg ttttacctga taatccatta gttgtaacac tgcctcccgc attactggag
      301 agtcaatatt caaatacctt aatcaccatc cagcgtttct tgcggaatgg ataacgggat
      361 acattctggc aggataaaac gttgttgtga cgaatatcac aaatctggct gctgaccgct
      421 actccatgtt gaggatgact ggcattccac catttgcaat atcacgccag atcccagtag
      481 gaaaatgggg attgataata caaaggcttg tatttgcaat ggtgataatt tcgtggttga
      541 taatattgat gtgatgaaaa atgtggagat gttaatcggc tacagagcga ttaaaagcaa
      601 atacctctcc atactgcaaa actttggagc aggtaatatt catcttgata atgctaaccg
      661 tgtctgggaa ttacgcagaa tctaaatctt cgcaggaaat ccccccttta tcgtgctgac
      721 ccgcattgaa atgaagtgtg ctttgttgga acaatacgac agattttgac ttgtttttta
      781 ttaaatatca ataatatatt tgtattcgtc gctcgtcagt attgaccatg agattagccc
      841 tgcgcgaaga cgtctgtgac tggtttatgg caaagggcga aacacgctcg ttgctggaaa
      901 atgttcatgc tgtgaatgaa aagaacaaag ctccgtcgat atcgacagag gtaatcaccg
      961 tattattaat gtggaaaaga ttaactttcg acagccagaa gaggggagga gatttgcgac
     1021 cattcctgga aaaatggaac catacttaaa aatatcgcta ctgcaatcct gaacgaagcg
     1081 gcgtagcatc ggcagtaaat cagtattcag ttcaggataa ttgatgctgg cgaaaaaaat
     1141 cgaacgagct ataattcagc aaccatttca caggtggatg aaataatgac gaatttaaaa
     1201 gcagttattc ctgtagcggg acttgggatg catatgttgc ctgccactaa ggcgataccc
     1261 aaagagatgc taccgatcgt cgacaaaccc atgattcagt acattgttga cgaaattgtg
     1321 gctgcaggga ttaaagaaat cctcctggtg actcatgcgt ccaagaacgc agtcgaaaac
     1381 cacttcgaca cttcttatga actagaatct ctccttgaac tgcgcgtaaa acgccagttg
     1441 ctggctgagg ttcagtccat ctgcccaccg ggcgttacca ttatgaacgt gcgtcagggc
     1501 gaagctttag gtttgggcca ctccattttg tgcgccagac ctgccattgg cgacaaccca
     1561 tttgtcgtgg tactgcctga tgtcgttatt gatgacgcca gcgccgatcc gctgcgttac
     1621 aaccttgccg ccatgattgc gcgattcaac gaaacgggcc gtagccaggt gctggcaaaa
     1681 cgtatgccgg gtgacctctc tgaatactct gtcatccaga ctaaagagcc gctggatcgt
     1741 gaaggcaaag ttagtcgcat tgttgaattt atcgaaaaac ccgatcagcc gcagacgctg
     1801 gactcagaca ttatggcggt tggtcgctat gtgctttctg cggacatttg gcctgaactg
     1861 gagcggactc aacccggagc atgggggcgt attcagctaa ctgacgcaat tgctgaactg
     1921 gcgaaaaaac agtccgttga tgcaatgtta atgacgggtg atagctacga ttgcggtaag
     1981 aaaatgggct acatgcaggc atttgtgaag tacggactac gtaacctgaa agaaggagcg
     2041 aagttccgca aagggattga gaagctgtta agcgaataaa aatcttaccg gatgtaacgg
     2101 tcaataagaa aaaccataac ggcagtgaag atttgtggca aaagtaaatt gcaacaaatg
     2161 ttcctgtcat tgtttcgtta taaaacacta taataacaat gagttagcaa taagatttta
     2221 gtcaaagttt tcctggattt ttcttgtttc cagagcggat tggtaagata atcgacggtt
     2281 cgatttcttg ggggttagtg tgtggataac gcacgttaaa ttgtagactg catatacagt
     2341 gctctggtag ctgttaagcc aggggcggta gcgtatataa attaattata tggttattaa
     2401 gaatattttt tggcaatttg cagagaagat tttaaggtta atttttgcga tttttattag
     2461 tgcgttgttg gcaagacatt taggtgtcag tggctttggt ctgtataact atattaatgc
     2521 gattatttcc gttgcattag ttgtaacttc gctcgggttt aatcgaattt tagttagaga
     2581 atccacttgt tgcaaagata aaattagtca caatgagttg atatcaacat cgttttattc
     2641 aagactgctt ttttcaatcc ttatattcgc tctcttatgg ggggggtaca gctatataga
     2701 taataaaaac tcaataatat atttaatatc cttcctttca gtaatttttg tttcatttga
     2761 tgtaattgac tatcacttac aaggacagtg tatatttaaa acagcaactc tatgtagagt
     2821 tgtgagtttt ttaattgtgt cctttataag aattttttta gttttcgaaa atgcaacgtt
     2881 gatatggttc tgtattacca tattatttga atatgccata tctgccattt tattatattt
     2941 gagctctaga tttatacata gaaacatttt tactattaag ataaaacacg tatcactaaa
     3001 taaagcaaag tatttgctga aggaatcgtg gtcagaaatt atagcaggat ttggagccat
     3061 tctgtttatg aaaatggatc aaatcatgtt acaatatatg cttaatgctg agagtgttgg
     3121 tgtttattct gctgccttaa ggataagtga atcatggtat tttttgccgg cagcaattgt
     3181 ttcgtcagta tttcctgtaa ttatgaataa atataataag gaaaatacaa atgagtcggc
     3241 gttggttgaa ttatttggtc atattatgac ccttcttgtc tggttatcta tctgtgctgc
     3301 gattactatt tctttgtttg gcccagcggt aatacattgg ttatttggtg atgaatatat
     3361 tagaagtgcc acaatattga tatatcatgt atgggcagga gtttttttga gcatggggat
     3421 tctttctggt tcatggttag tagctaaaaa aaaattaaaa ttaaatttat accgaaatat
     3481 cggaggtttg atagtaaatt tttttctaaa tatcatatta ataccagtgt atagtggtgt
     3541 tggagctgct attgcaacaa tttcaggatt tgcattcgct ttcttgtttt ttgatttgtt
     3601 tcataaagag ttaaggggga tgtttttttt aaaaataaaa tcactttccc cgattaattt
     3661 aattcaagca atcaggtttt tatctaacat aagattaaaa tatctatagt ttataatcat
     3721 atgtcatgat tggggcatat cacggtgcgc cataggcaca aaggtcaggg aaagatcatc
     3781 agaggtactc tttttgtact aaaaaatttg catttcatac tcactaattg agatattcct
     3841 tattacctaa actgtttttc atagcttata tattatcaaa tactcctttt atgaataagg
     3901 agaacaaaat ggaacttaaa aattgatgga gcatatttct gctatcctcg attacagaca
     3961 agcttggaaa gtggaatata aattgttaga tattctactg ttaactattt gcaccaccat
     4021 ttctggtgca gaaggttggg aggatataga taatttgggt gaaacatatc tcgatttttt
     4081 gaagcaatat agttgatgtt gaaaatggta tttctgttca tgatatcatt gcgagagtta
     4141 tgtcctgtat tactcttgct aaatcttatg aatattttat taactgaata ttggtatatt
     4201 tatatgttga ctgtttaaac caagggcagg gtatttaata tattaaacat atctacggat
     4261 acaggttata actattcttg ttgttttttt tgaaattgat tttctgttat aactcctgat
     4321 taaagcaagt gttttgaaat gtgattttct agttataatt cttcgataaa acatagttct
     4381 atttccagta tgttatgagt caaaactctt taaacaaaga taaatagatt aacttaaatt
     4441 gcatataaag tgatattatg aatatgaaaa atataattga aagaattaaa ccattgttct
     4501 ttgatggctc cagacacttg gtgagacctg ttgttttaca gttcccggta attgatattt
     4561 gtaattcaaa atgccaaatg tgtagaattt gggaaaataa aaaaagtgta gacatttctg
     4621 tagagcaatt aaaaaaaggg atacagaatg agctattcaa agatgttatg tctattgggt
     4681 ttaatggtgg agagcccact cttcgtgagg atttatcgga ggttatagaa acctgcctat
     4741 attctttgcc aaagctaaaa caaatatcat tgattaccaa cggatttaaa tatgatcaag
     4801 tcattcatca gattgaaaaa atcggtaagt tgattaagtc taaagatata ttttttgatg
     4861 taatggtttc tttggatggt tttggtgtta tccatgataa agtacgagga agaactggaa
     4921 gttttaaaca tgctcaacat gtattaactt ttctaaaaaa cgctgattat gtaagcaata
     4981 tacgaattgg ttgtacagta ataaaagaaa atgtttatta cttgcatgag ttgttggagt
     5041 tttgcataag agaaaagctg tacgtaaaat atagacaagg cgtgccacac aaacgattat
     5101 acacagaaaa tttaattgaa ccatattctt taacctttga agaaaaatat gagttcgttg
     5161 agtttttgga gggaataata aaatattatg agccaagttt attacagaaa catttttatc
     5221 gctcattaat taatcaaatc atatatgagc atccaagaac agcaggatgt gattggagat
     5281 atcgtggtgc gacgataaca gcgaagggtg aattggcata ttgtgcaata gaaagcaaaa
     5341 cgctgatgaa ggatatatct gatggtaatg catatcaaat atattttgat aatgaaaatc
     5401 atttgaatga tattaaagaa aataagtgta aaaactgcca tcatgattat gttggtctgc
     5461 ctcctcctgc tgaatacaga aaaatatttc tatcaagcat aaatgaaaag tggaaaatta
     5521 aagataaaat aaaaaaaatt ccgggaggga ggggcattaa taagataata aatgaataca
     5581 agtatagtac atgtataaat caatatcgaa atattcattc tactcaagtt caagatgtat
     5641 cattacgtgg aaacacaagg gtgctaattt gtgggtggta cggaactgaa actttgggag
     5701 ataaagctat cattggaggc attatcaatt ctgtgcgcag tagtttaggt tatgaggttg
     5761 ttttttatat cgcatcatta tatccatata tcacagaaat gactcgccgg cagatgcctg
     5821 aattgaaaaa ctgccaaata gtaaatatac aacaagcaat cttgttagca aaagatatga
     5881 cttatgttgt ttttggtggg gggccattaa tggcgataga tgatattgct ccaatgcagg
     5941 ttatatttga aaaagcgaaa caagcaaaag taaaaactgg aataatggga tgcggaatag
     6001 ggcctttagg tgataagaga tataataata gcattaaaac tattcttgag ctttctgatt
     6061 ttcgtatata tcgagatcaa aaatcaaaaa gtgaagcatt aaggttagga gtaaatgctg
     6121 aaaatgatat tgttgccgag gatccagcat tttcatggat aaaatcttat tatgaacagt
     6181 cggtagtggg tggaagacat cgtaaagcgt atatcaatga tgaaaaagaa atattattat
     6241 taggtttaag agattttcca tggagagagt atgcaaaaca tctttcagaa aaagaatgct
     6301 taagcattaa agataatttt gaggaagtat tagtatcagc tctaaaagaa gtagtaaaaa
     6361 agagggataa tttaataatt agaccattac ctatgtgtac taatcacttt ggttcagatg
     6421 atcgttggtt ttatcgtcga ttatttagag attctggtat tccatcagaa aatttagatt
     6481 tttctttatg tggtagagaa ttagcgccga ctgaatattg taaggcattt ttggatgcca
     6541 atgctcttct tgctatgcgg ttccattcct tagtttttgg acttggatta ggtactccat
     6601 cgttagctct tgattatacg ttgggaaagg gaaaggtcaa atcattggcg gagcgttttg
     6661 gtgctaatgt tatatcgtta tctaaaataa ataaagaaga actactctta gaaatagaag
     6721 acttattgga acataactca caggaaagta atgtctctat tttagaaagt attaaatttc
     6781 agcaaagcgt agataccttt atgaagaggg attaaattga aaattctaca tatatgctac
     6841 agtgattgtt taggtggggc agccagagct gcaaaccgtt tacatcaggc acaaagaaaa
     6901 ctgggaataa atagctttat gcttgttgtt gataaatata cagatgatga ttatgtcttg
     6961 gagcttaaca agctaacaaa atttcgaata aaaatactta atatgatttc tggctacata
     7021 ttaaggcacc taatacaaaa gaatcctgtg aagcactcgt taaatatttt ttcagcaggg
     7081 gttgtgaatg caataaataa attaaacccc gatatagtta atattcattg gattggtgat
     7141 aatatgcttt caatcaaaga aatatcaaaa ataaaatcgc ccataatatg gacgatgcat
     7201 gatatgtggg ctttttctgg ttgtgaacat tatgatagct ttcctggaat aagacgttat
     7261 gaagatgact ataacaaaaa agatgggata tttgatctaa atagaatcat ttatcgatat
     7321 aaaaagaaaa aatggtccca tataaatatg cagtttgttt ctccaagtcg gtggttggcc
     7381 gagtgtgcct gtagaacgag tctcatagaa ggaaaaagag ttcatgttat tcagaataca
     7441 atagatcatg ttgtatttga taaagtggat aaagtatgtg caagaaaatt gttgggcctt
     7501 ccccaaaacc gaaaacttat cctatttgga gcgatgtctg gtactgcaga tcatcgaaaa
     7561 gggtttcatt tgctaaatga agcgttgaaa tatttatcgg aaaaatatgc tactgaatat
     7621 gggcttgtta tttttggtga taaaaaagat ggcgatgata tccatggatt cccaacttat
     7681 agattaggta cattttatga tgagctatct ataaagttga tatattcagc cgcggatgta
     7741 tatgttgcac cttctttaca agataatcta cctaatacat tagtcgagtc attggctgtg
     7801 gggactccat gtatcgcttt taatattgga ggtatgcctg atttaattat aaatagcaaa
     7861 ctagggggat tagtcacaga ggttgatagc agagcattat ctattgctat tttggatgta
     7921 ctaaatagaa atatagattc taatataatt agaaatattt cgttagcaga gagggccgag
     7981 gataatattg ctcataaata tgaggatgtg tatctcaatg cataaaaaac tatattatta
     8041 ctttttaatt gtttcacctt tgatatatct aacaggtggt attgaacatg ttaccccgtt
     8101 atcaatagcg gggtatttgg atttaccgtc attgtctctg attaattata tattctttct
     8161 atggtgtgtt ttttttctgc acttttattc atttgggaaa aatatattat ggttgtatct
     8221 tttagatata gtaataataa tgattatatt tagagaaagt agagttgata gcatcttttt
     8281 ttctactctg tctcaatttt taattctttc accattgttt cttcttgtcg gttgtaaaat
     8341 tcatattgga tatgaaagga tcaaaaaaat tagctatata tttgtttttt atttaatatg
     8401 tagtatactt cttcactata tatttatcat gtactataaa tatataggtt tttctttacc
     8461 aaatgagaga gcagtaggta tttttaaaaa cccaaatcat ttagctgctt tttctatact
     8521 cttactaatt tttctctata atttagcttt tattaaacta gttgacgtaa aaaaaatttt
     8581 ctttgctgag ctcattgttg cagttactgt ttatttatct ggtagtaggt cttcgcaggt
     8641 tttttttata atattattat tgatacattc gttcttgtat aacaaacgtt tcttttcttt
     8701 atattgctgt ttagccgttt tgtctgttgc ttatgtaggt atgtcagata tcggagtaga
     8761 taagatagaa caattgttaa caaagcgaga gacgaaagat attgctgagg ctggtaatat
     8821 gcggatgtcg attctttttg atatgcttga gaattttaat tttcgcgaaa tattaatagg
     8881 caagggtagt gggcaaggaa ctgctgtata tataactaac caaagtaata ataatgagaa
     8941 aattacgtgg ttggacagta atgttaatac actaacctat acgtatgggg ttgttttttt
     9001 aattttttca ttggctgcaa tgctgttaaa aatgcttttc ttagctctta tgaaaagaac
     9061 aggtgaattt tatatatttt tcttcgtttt ttattttatg tggtttatta acattgggga
     9121 attttttcct cttattttta tgttgttaat tcaagtaagt acatctaata tgggacagca
     9181 gcatgatatc aataattact ataaattata ataataaaaa cgaactttat cgtacattat
     9241 catcaattga tagtcagaaa ttaaagagtc atatagaggt tattgttata gatggtggtt
     9301 ctaccgatgg ttcgcaagaa atagtatcta actcatttaa acatttggtt acatatttca
     9361 tttcagaacc tgatatgggt atctatgatg cgatgaataa aggtatacta ttgtctaata
     9421 aagaatggct gtacttttta aatgcgggag atattttttt ctctgatgat gttttaaata
     9481 aattatatga ctttattata aatagtaatt tgactaattt gatatatgca ccttatatta
     9541 gtgatgggaa aatagataat actcaacttt tttctttgtc ctatctttct tcccatatga
     9601 ttaatcatca aagtatgctt ctacacaagg aactgtttca aaaacagtta tatgatatta
     9661 actataggtt ttgtgctgat tatgcattat tattaaattt gtttgataat atcaaaccgg
     9721 taaagacgga ctttattatt gttgattatg attcgaaagg gatcagttca ttagatgata
     9781 ataaagtatt tatatggaaa gaaagaattc ttgctgttct gaaatcaaac ttaagctttt
     9841 ataaaaagct atttttattg aaaagggctt ttggggtata cccatatcat ttattaaaaa
     9901 gtaaattaaa atgcatgaag aatataatga cacattgtcg atagtgtttt gtgctttata
     9961 tgatgactgg ttgaaaaata aagaacttat acaccgctca tttgagcatt tcgtaaaaat
    10021 atgggaggag cgatgttttt ttattttaat tatacaagat gcgaaacagc gaaagattga
    10081 gttggcattt aaaaacatgc atatttttta tatgcaagaa agaggtattt cgttagcaag
    10141 aaatcgaggt gttgaggaag catgcacatt aaacagtcgt tggattatat tccatgatgc
    10201 atctatttgc tggactgaag aagctgtggg ctttctattt aaaaatagat tctccaaaat
    10261 ttcgcctaaa gttaaactaa aattccaatt aattgaggaa aagccatcaa cagatgttca
    10321 aggtgaaata agaaaaatta atccagttta tgatacatat gttggttctg ttttatttga
    10381 agttcagtca atcgctaaaa taagatttaa catatttcat gggcctggtc agaatactat
    10441 ttataaaagc ggggaggatg ttctattctt ttttgattat ttttctatga agaaaaattt
    10501 ttgtgtgtat gagtctaaaa cagcgtatat ttatcatcca ccacgagaaa aagatttttc
    10561 taaacataaa atatatgcga aaggacaagg gcgggtcttt aaaattttat taaaagaata
    10621 tttttcaatt cgtttgctaa tggattgcat tctttttttt gggaatgcgc ttgtgagatg
    10681 tatttttttt aggaaaaact ctttttatat tcttaaagag agatgtgttg gttttttttg
    10741 tgaggattaa aatgaaaaaa gcattaatta caggtattac tggtcaagat ggttcttacc
    10801 ttgctgaatt tcttctcgaa aaaggatatg aggttcatgg tataaaacgt cgagcatctt
    10861 cttttaacac cgatcgcgta gaccatatat atcaagatcg ccacaatgat agtccaaatt
    10921 ttctcctaca ttatggtgat ttaacagatt catcaaactt gattcgtctc attaaagaaa
    10981 tccaaccaga cgaagtttat aaccttggcg ctcaatctca tgtagcggtt tcattcgaat
    11041 cacctgaata tactgctgac gtagatgcga tggggacttt acgtttgctg gaggcgattc
    11101 gtatttgtgg gctagagaaa aaaacacgtt tctatcaggc atcaacttct gaactcttcg
    11161 gcttagttca ggaaacccct cagcgagaaa caacaccatt ctaccctcgt tcgccttatg
    11221 ctgttgcgaa gatgtacgct tactggatta ctgtaaacta tcgtgaatcc tatggaatgt
    11281 acgcctgtaa cggtattctc ttcaaccatg aatccccacg tcgtggtgaa acattcgtaa
    11341 cacgtaaaat cactcgtgct attgcaaata tttcgcaggg aatcgaaaaa tgtctctacc
    11401 ttggcaatat ggattcactg cgtgactggg gacatgcgaa agattacgta cgtatgcagt
    11461 ggatgatgtt gcagcaagat caccctgaag attttgtaat tgctaccggt aaacaaattt
    11521 ctgtccgtga atttgtccga atgtcagcta gagaagttgg cctagagctt gagttctctg
    11581 gtgaaggtgt tgatgaaatc gcgaccgttg taaataaaac atctgactgc gcgattggcg
    11641 taaatatcgg cgatgttatt gttcgtgtcg atcctcgcta cttccgtcct gctgaagttg
    11701 aaactcttct tggtgatcca agtaaagcta aaaaagtatt gggttgggag ccagagatta
    11761 cggttgaaga aatgtgtgca gaaatggttg ctagcgattt ggcgaaagca aaacagcatg
    11821 cactactcaa aagtcatggt tatgatgttg cggtttctct ggagcgttga ggtatgacaa
    11881 agaaacgtat ctacgttgct ggtcatcgag gtatggttgg ctctgctatt tgccgtcaat
    11941 tatcactgcg tgatgatatc gaattagtgg tcaaaacaca caaagaactc gatctgaccg
    12001 tgcagaagga tgttgaggta ttttttgagc aagagagaat tgatcaagtt tatcttgctg
    12061 cggcgaaagt aggaggcatt tatgccaata atacatttcc ggcagaattc atctaccaga
    12121 atctcatgat tgagagtaat attattcatt cagcccacaa agctgggatt caaaaattac
    12181 tttttctagg ctccagctgt atttatccaa aatttgcaga gcagccgatg aaagagtctg
    12241 cgcttttaac tggaatactt gaaccaacta atgaaccata cgcaattgct aaaattgcgg
    12301 gcataaaatt atgtgaatct tataatcgtc aatatggacg tgattatcgc agtgtaatgc
    12361 ctactaatct ttatggtatg aatgataatt tccatcctga aaattctcat gtcattccgg
    12421 ccctcatgcg taggtttcat gaggcaaaag agagtggtgc accagaagtc attgtctggg
    12481 gaacgggaac accgatgcgt gagtttttat atgttgatga tatggctgcc gcgtcggttc
    12541 atgtaatgga actagacgac gtaatttatc aacaaaatac acagccaatg ttatcgcata
    12601 ttaatgttgg tactggtgtg gattgttcca tacgtgaaat ggctgaaaca atggcttctg
    12661 tagtaggttt ccagggaaaa attgtttttg actcaacgaa accagatggt acaccgcgta
    12721 aacttatgga cgttactcga ctcaaaaatt tgggttggca atatcgctat aatttacatg
    12781 aaggtttatc attaacgtat aaatggtttc ttgcgaatat taattctttt cggggatagc
    12841 tatgaaaaag agattagaat atgagttatt taaaacaata gttgagcata ctcctctaat
    12901 ctcgattgat ctcataatta gaaacgataa aggagaggcg ctgcttgggc agcgcctgaa
    12961 tcgcccggca caaaattatt ggtttgtgcc gggggggcga atttttaaag atgagtcatt
    13021 cgaggcggct tttaaacgga taacatttga agagttgggc gttcaaacca gtcttaatga
    13081 agccttattt cttggtgtgt atgaacattt ttacaatgat aacttttctg aaacagaatt
    13141 ttctacacac tacgtagtac atgggtatga aatccaacta aatccacagc aacttcacct
    13201 accaacgatc cagcataact cctacaagtg gtttgatata gaaacgttgc ttaatagcac
    13261 tgatgttcat caatacacca aaaattattt tatttcaagg taatagatat gcttcttccc
    13321 gttgttatgg ccggtggttc tgggaccaga ttatggcctc tttcacgtac gctttatccc
    13381 aaacagtttc tgtctttaaa tagccgtttg accatgttgc aagagacatt gcgccgactt
    13441 gacaaggtcg aacataaacc tactttggtc atttgtaacg aatcacatcg ctttatcgtt
    13501 gctgaacaat tgcgtaaaga gggtttaaag catagcggta ttttgcttga gcctgttggt
    13561 cgtaatactg cgccagctgt tgcgttggca gcactccagg caaaagcatc tggcgaagat
    13621 cctatactcc ttgtacttgc tgctgatcat gaaattcagg atgaaggtcg ctttattgct
    13681 gctgttcttg cggctaagaa ttttgctgaa cagggtaagc ttgttacatt tggtattgtt
    13741 ccagctgctc ctgagacggg ttatggttac atcaaggttg gtgaggagct agatgggcag
    13801 ggttacaaag tcgcggcctt tgttgaaaaa ccagagttgg atttagctaa acagtatctg
    13861 gccgatggtg gctatctttg gaatagcggg atgttcatgt tcagggcatc tatatttctt
    13921 gatgaactga aaaagtttcg tccagatatt ctggccagtt gccaacaatc cctgtcctct
    13981 tcgacacaag atttagattt tattcgcctg gataactctt cattttcttg ctgtcctgaa
    14041 gagtctattg actatgctgt tatggaaaaa acagccgaag ctgtcgtcgt tccattaaat
    14101 gcgcaatgga gtgatgttgg gtcatggtct gctctgtggg aaatcagttc aaaagaccaa
    14161 agcggcaacg ctattcgtgg tgacgttttg attgaagatg ctacagatag ctacctttat
    14221 tcgcagcata ggcttattgg tgctgttggt gtaaaagatt tggttgttgt tgaaacgaaa
    14281 gatgcagtat tagttgctca taaagacaaa gttcagcaag ttaaaaatat tgtcgctcaa
    14341 cttaaaaaga ataatcgaac agagtattta cagcatcggg agatttttcg tccttggggc
    14401 agccatgaca ctatagctga aggacaacgc tttcaggtaa aacatgtgat tgtattacct
    14461 ggtcacatca ctgctaagca aatccattat catcgtactg agcattggat tgttgtctcg
    14521 gggacagcta aagttcatct tgaagataag acttatcttg tttcagaaaa tgaatcaaca
    14581 tatataccta ttggtattcc gcatgccatt gaaaaccctg gcaagatccc acttgaaata
    14641 attgaagtta gatcaggagt ctacttggaa gaggatgatg tcataagagt ttcttcttct
    14701 ggagtcggat actaatgcga atttctatta tcacggcaac ctataatagt gaaaaaactc
    14761 tccttgatac attactctct ctggaaaaac aaacacatcc agatatcgaa tatatagttg
    14821 tagatggggc atcgaaagac aatacaatca agctaatcaa gagtaattgt acaagagttt
    14881 caaaaatcat ttgtgaacct gacaatggca tttatgatgc gctgaataaa ggtattcaag
    14941 ctgcttctgg tgatgttatt ggttttttgc attctgatga cttacttgct tatgatgatg
    15001 ttattgcaga tatagcaaaa gcatttgaac gttcagggtg tgatgctgtt tatggtgatt
    15061 tggagtatgt cgcccaaaat gatacgacta aacgtattag attatggaaa agtggagcat
    15121 ttagccgttt gaagatgaaa ctgggttgga tgccaccaca cccatcattt tatatgaaac
    15181 gtgattgtta tggtcagttt ggttgttttt cattagatta tcgaatatct gctgattatg
    15241 actcattgtt acgttatatg ttaaaacagc gtatttcaat aacgtattta ccaaaagtat
    15301 tagtgaagat gcgtgttggc ggaattagca atcgctcagt atcttccatg gtcaacaagt
    15361 caatggaaga tattcgtgtt atgaaacaca atggtattat ttggccaata gctttagcgt
    15421 ataaaaatct atccaaactt ccacaattca ttaaaaagta attctcatgt taaatgctaa
    15481 aaaaatcatt accggtagca atattgcttt cggaactagt ggcgcgcgtg gtctcgttgt
    15541 cgattttact catgatgttt gtgcggcgtt cacccacgct ttcctttccg ttatacagaa
    15601 taactttaac ttccgtattg ttgccttagc cattgataac cgcccaagta gttacgaaat
    15661 agctcaggca tgtgcatatg ctataaaaca acatggattt gaggttgaat atcatggtgt
    15721 cattccgact cctgcattag ctcattattc gatgcagaaa aatattccct gcataatggt
    15781 cactggaagt catatacctt ttgatcgtaa tggtttgaaa ttctaccgac cagatggtga
    15841 aatcacaaaa gaggatgagt tcgcaattgt aaatagtgaa tatacatttt ctcctgtagg
    15901 tgtattacct catcttgaaa caagctctca aggtgcggac tgctacctgg aacgttatgt
    15961 ttctcttttt aattctgata ttttaaaggg gaaaagaata ggggtatatg agcattctag
    16021 tgcaggacga gatctatatg cacctctttt taatcaattg ggggctgagg tcatatccct
    16081 tggtagaaat gatgagttcg ttcctattga tacggaagcc gtaagcgatg aagatcgtat
    16141 acttgcgcga gagtggtcta aaaaatataa tcttgatgct attttttcaa cagatggtga
    16201 tggtgatcgt cctttagttg ccgatgaaaa tggtgaatgg ctaagaggcg atattctggg
    16261 attacttact gctattgagc ttaatatcaa ggcgttggct attccagtga gttgtaatac
    16321 agcaattgaa cagtctaata aatttgcaag tatacaacga acgaaaatag gttctcctta
    16381 tgtaattgca gcgtttgcag ctcttggtaa acaatttgat tcagttgctg gttttgaagc
    16441 taatggtggt tttctgctcg cctctgattt acaaattaat ggtaaagaat taaattcatt
    16501 gcctacgcga gatgctgtat taccggcatt aatgcttcta atagcttctc gtaatagtac
    16561 tatctctcaa ctggttaata atcttccaca gcgattcact tggtcagata gagttaaaaa
    16621 cttcccttca gagtcaagtc agcaaattat aaaaaatgcc ttatcggcac ctaataaatt
    16681 cttaaataga ttagggtatg aatcattatc ctgttcagct attgatgaaa cggatggtgc
    16741 aagatttact ttaactaatg gtgatattat acatctccgt ccttctggga atgcccctga
    16801 actccgttgt tattctgaag ccagtaatga aaatcaggcc aagcaatatg ttacgaaagt
    16861 gcttggaaac attacctctt taatttcttg atgttatagg tttatctacg cttatatgtg
    16921 tgcgtaggtt tgattacaag tggatgccgg tatacaggat gaagaacggt attcgttgca
    16981 ttaatgaaat tcagcactac acacattcgt gcaacttgag ataacatctc aatcacattc
    17041 aagccgcgca accccgcggt gaccacccct gacaggagta aacaatgtca aagcaacaga
    17101 tcggcgtcgt cggtatggca gtgatggggc gcaaccttgc gctcaacatc gaaagccgtg
    17161 gttataccgt ctctattttc aaccgttccc gtgaaaagac ggaagaagtg attgccgaaa
    17221 atccaggcaa gaaactggtt ccttactata cggtgaaaga gtttgttgaa tctcttgaaa
    17281 cgcctcgtcg tatcttgtta atggtgaaag caggtgcagg cacggatgct gctattgatt
    17341 cccttaagcc ataccttgaa aaaggcgaca tcatcattga tggcggtaac accttcttcc
    17401 aggacaccat tcgtcgtaac cgtgagcttt ctgctgaagg ttttaacttc attggtacgg
    17461 gggtttccgg tggtgaagag ggcgcgctga aaggtccttc tattatgcct ggcggtcaga
    17521 aagatgccta tgaactggtt gcgccgatcc tgaccaaaat tgctgccgta gctgaagatg
    17581 gcgaaccgtg cgttacctat attggtgcag acggtgctgg tcactatgtg aagatggttc
    17641 acaacggtat tgaatacggt gatatgcagt tgattgctga agcctattca ctgctgaaag
    17701 gtggtctgaa tctctccaac gaagagctgg cgcagacctt taccgagtgg aataacggtg
    17761 aactgagtag ctatctgatc gacatcacca aagatatctt cactaaaaaa gatgaagatg
    17821 gtaactatct ggttgatgtg attctggatg aagcagctaa caaaggcacc ggtaaatgga
    17881 ccagccagag tgcgctggat ctcggtgagc cgctgtcgtt gattaccgag tctgtgtttg
    17941 ctcgttatat ctcttctctg aaagagcagc gtgttgccgc gtctaaagtt ctgtccggtc
    18001 ctcaggcaca gccagcaggc gacaaagcgg agttcatcga gaaagttcgc cgtgcgctgt
    18061 atcttggcaa aatcgtttct tatgctcagg gcttctctca actgcgtgcc gcgtctgaag
    18121 agtacaactg ggatctgaac tacggtgaaa tcgcgaagat tttccgtgct ggctgcatca
    18181 tccgtgcgca gttcctgcag aaaatcaccg atgcctatgc agaaaatcca aaaatcgcta
    18241 acctgctgtt ggctccgtac ttcaagcaaa tcgccgacga ctaccagcag gcactgcgtg
    18301 atgtcgtcgc ttatgcagtg caaaacggta ttccggttcc aaccttctct gcggcggttg
    18361 cttattacga cagctatcgt gccgctgttc tgccagcgaa cctgatccag gcacaacgtg
    18421 actatttcgg tgcgcatact tataagcgta ttgataaaga aggtgtgttc cataccgaat
    18481 ggctggatta atctgattta atcagcaata aattgaggcc cggcatatat tgccgggttt
    18541 ttttgctaat aatcctatag attaaatttc attgcttaat attaacttaa taaatatcag
    18601 ctactcttat aagtaaagtc tgattttgtt gttacgctga tgctgggatc agcgtagcgc
    18661 taaaaatttt aacttctatc aggatgctaa aacatcatga ttcacagtta agttaattct
    18721 gagagcatga aatgaaaatt accatttccg gtaccggcta tgtgggcctg tcgaatgggc
    18781 ttctaatcgc acaaaaccat gaggttgttg cattagatat tttgccttca cgtgttgcaa
    18841 tgctgaatga tcggatatct cctgtcgttg acaaagagat tcaaaatttt ttgcagtcag
    18901 atgaaattcg atttaacgcc acgttagata aaaaagaagc ctatcggaat gcagattacg
    18961 taatcatcgc aacgcctaca gactacgatc caaaaacaaa ttatttcaat acgtctagtg
    19021 tggaatctgt cattcaggat gttattgaaa taaacccatt tgcggttatg gttatcaagt
    19081 ctactgtccc ggttggtttt accgcggcaa tgcagacgaa atatcatact gaaaacatta
    19141 ttttttcccc agaattcctc cgcgaaggca aagcccttta cgataacctt ca
//
LOCUS       DAJTNA010000068        19136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTNA010000068 DAJTNA010000000
VERSION     DAJTNA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19136
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.069"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..189)
                     /locus_tag="OO161_002024"
     CDS             complement(<1..189)
                     /locus_tag="OO161_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5463093.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTV"
     gene            290..889
                     /locus_tag="OO161_002025"
     CDS             290..889
                     /locus_tag="OO161_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5463094.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            997..1764
                     /gene="tpiA"
                     /locus_tag="OO161_002026"
     CDS             997..1764
                     /gene="tpiA"
                     /locus_tag="OO161_002026"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5463095.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(1819..2574)
                     /gene="cdh"
                     /locus_tag="OO161_002027"
     CDS             complement(1819..2574)
                     /gene="cdh"
                     /locus_tag="OO161_002027"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5463096.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(2681..3670)
                     /gene="sbp"
                     /locus_tag="OO161_002028"
     CDS             complement(2681..3670)
                     /gene="sbp"
                     /locus_tag="OO161_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5463097.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(3990..4952)
                     /gene="pfkA"
                     /locus_tag="OO161_002029"
     CDS             complement(3990..4952)
                     /gene="pfkA"
                     /locus_tag="OO161_002029"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5463098.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5133..6035)
                     /gene="fieF"
                     /locus_tag="OO161_002030"
     CDS             complement(5133..6035)
                     /gene="fieF"
                     /locus_tag="OO161_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5463099.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHMISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6183..6707)
                     /gene="cpxP"
                     /locus_tag="OO161_002031"
     CDS             complement(6183..6707)
                     /gene="cpxP"
                     /locus_tag="OO161_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5463100.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSDDNWHPGEELTQRSTQRS
                     TQRSTQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENA
                     VRAQAEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKS
                     SSLKLLSSSNSRSQ"
     gene            6857..7555
                     /gene="cpxR"
                     /locus_tag="OO161_002032"
     CDS             6857..7555
                     /gene="cpxR"
                     /locus_tag="OO161_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5463101.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7552..8925
                     /gene="cpxA"
                     /locus_tag="OO161_002033"
     CDS             7552..8925
                     /gene="cpxA"
                     /locus_tag="OO161_002033"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5463102.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9031..9705)
                     /gene="yiiM"
                     /locus_tag="OO161_002034"
     CDS             complement(9031..9705)
                     /gene="yiiM"
                     /locus_tag="OO161_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5463103.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(9854..10836)
                     /gene="kdgT"
                     /locus_tag="OO161_002035"
                     /pseudo
     CDS             complement(9854..10836)
                     /gene="kdgT"
                     /locus_tag="OO161_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(11096..11716)
                     /gene="sodA"
                     /locus_tag="OO161_002036"
     CDS             complement(11096..11716)
                     /gene="sodA"
                     /locus_tag="OO161_002036"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5463104.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12000..13034
                     /gene="rhaT"
                     /locus_tag="OO161_002037"
     CDS             12000..13034
                     /gene="rhaT"
                     /locus_tag="OO161_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR5463105.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13031..13897)
                     /gene="rhaR"
                     /locus_tag="OO161_002038"
     CDS             complement(13031..13897)
                     /gene="rhaR"
                     /locus_tag="OO161_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5463106.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQAWQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(13953..14789)
                     /gene="rhaS"
                     /locus_tag="OO161_002039"
     CDS             complement(13953..14789)
                     /gene="rhaS"
                     /locus_tag="OO161_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5463107.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15077..16489
                     /gene="rhaB"
                     /locus_tag="OO161_002040"
     CDS             15077..16489
                     /gene="rhaB"
                     /locus_tag="OO161_002040"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5463108.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSMTLREIHRFNNDLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            16486..17745
                     /gene="rhaA"
                     /locus_tag="OO161_002041"
     CDS             16486..17745
                     /gene="rhaA"
                     /locus_tag="OO161_002041"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5463109.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            18012..18836
                     /gene="rhaD"
                     /locus_tag="OO161_002042"
     CDS             18012..18836
                     /gene="rhaD"
                     /locus_tag="OO161_002042"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR5463110.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAIPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            18846..>19136
                     /gene="rhaM"
                     /locus_tag="OO161_002043"
     CDS             18846..>19136
                     /gene="rhaM"
                     /locus_tag="OO161_002043"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5463111.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSEL"
BASE COUNT         4723 a         5039 c         4863 g         4511 t
ORIGIN      
        1 aacagttact gccgctgttg gccaaagcaa gcacacaagg caaaacgccg cggtctgaat
       61 atcgagtaaa tttttacccg ccagtgagaa aagcaccagc atgcctgcga accacaggcc
      121 tatccaaatc cagaaatcac ggcgtccaat acgcccttta aatgagaata accattgctg
      181 tatggtcatg taagttcctt gatggttgtc ttttccagga ttctaccctt ttgacaaggg
      241 cgacagatat cgttttaatc ggagccagtc ataacaaaag gtactgtcaa tgaagccagg
      301 gtgtacgctg ttttttctct tatgttctgc attaaccgtt acaacaacgg cgcatgcaca
      361 aacaccagat acggcaacga ccgcgcctta tctgctggct ggagccccta ctttcgatct
      421 ctccatcagc cagtttcgag aagactttaa cagccagaat cctagcctgc cactgaacga
      481 atttcgtgcc atcgacagca gtcccgacaa agccaatctc actcgtgctg caagtaaaat
      541 taacgagaac ttgtatgctt ctacagcgct ggagcgcggt accttaaaaa tcaaaagcat
      601 tcaaatgacc tggctaccca tccaggggcc agagcaaaaa gcagcgaaag cgaaagctca
      661 ggaatacatg gcagcagtga tccgcacact caccccatta atgaccaaaa cacaaagcca
      721 gaaaaaactg cagtcgctac taacggcggg gaaaaacaaa cgttattaca ccgagacaga
      781 aggtgcactg cgttatgttg tcgcggacaa cggcgaaaag gggctgacct tcgctgttga
      841 accgattaag ctggcgctat ctgaatcgct tgaaggtttg aataaatgac aaaaagcaaa
      901 gcctttgtgc cgatgaatct ctatactgtt tcacagacct gctgccctgc ggggcggcca
      961 tcttccttta ttcgcttata agcgtggaga attaaaatgc gacatccttt agtgatgggt
     1021 aactggaaac tgaacggcag ccgccacatg gttcacgagc tggtttctaa cctgcgtaaa
     1081 gagctggcag gtgttgctgg ctgtgcggtt gcaatcgcac caccggaaat gtacatcgat
     1141 atggcgaagc gcgaagctga aggcagccac atcatgctgg gtgcgcaaaa cgtggacctg
     1201 aacctgtccg gcgcattcac cggtgaaacc tctgctgcta tgctgaaaga catcggcgca
     1261 cagtacatca tcatcggcca ctctgaacgt cgtacttacc acaaagagtc tgacgaactg
     1321 atcgcgaaaa aattcgcggt gctgaaagag cagggtctga ctccggttct gtgcatcggt
     1381 gaaaccgaag ctgaaaacga agcgggcaaa actgaagaag tttgcgcacg tcagatcgac
     1441 gcggtactga aaactcaggg tgctgcggca ttcgaaggtg cggttatcgc ttacgaacct
     1501 gtatgggcaa tcggtactgg caaatctgca actccggctc aggcacaggc tgttcacaaa
     1561 ttcatccgtg accacatcgc taaagttgac gctaacatcg ctgaacaagt gatcattcag
     1621 tacggcggct ctgtaaacgc gtctaacgct gcagaactgt ttgctcagcc agacatcgac
     1681 ggcgcgctgg ttggcggtgc gtctctgaaa gctgacgctt tcgcagtaat cgttaaagct
     1741 gcagaagcgg ctaaacaggc ttaagtctga caggtgccgg atttcatatc cggcacttac
     1801 tttccttaac tcttcgcctt aacgcaaaat ctcacactga tgatcctgaa tttcctcggc
     1861 tgaagcacgg ttaagcgtca gtagatttcg ttgtgtcgcc agcaatacaa atgagttatc
     1921 actctgccgt accatcgcca gcccgtagct tcccatatgt tcccgcgcct caggtacttc
     1981 ttctgccagc atcataaatg ggctgcgttg taccagttcg ctttccgtta cccgacgcgc
     2041 caggtattca tgcccgcgca aaccacctgg cagtggcaac cagcggctgc tgatgttcgc
     2101 cagattgtta tccagttgtt cgcgcacatc aggacgaata caagagatat gaatatgaaa
     2161 atggttttgc gtacgcccgg tgcgggagtt gatcgccaaa gaaaccgcgc gatcgggaac
     2221 cggctggccg tactttttgc tcataaaatc acgcgcctgc caggccaacc aaaagaagtt
     2281 cggcgttgaa ggatcggtca acaaaggact ttcagtaccg ttaatacgat acgttggcat
     2341 caacagatat tgcagtgggc cattaagatc ttttaaaacc acgtatccgg cattgggttt
     2401 gacttccgca catggcgaag gattttgatt ttgctgctga ttgggcaaac attcctcaag
     2461 gacaatctta cgtaatgtat ccgactcttc accggttaat ttccagtaac caataccggc
     2521 agccacaacg gcgataacta tcatcaccaa aaaaagaaga cccgcttttt tcatcttttt
     2581 ttccctgtac ctcaaagaga tgcaagggta acgcaaaatc gtgacaaata aaaaacccgg
     2641 tcgcgagaaa ccaccgggtt tgataattat cctggggaaa tcagcgtttg ctgatctgat
     2701 cgaacgtacc gccgttagca aaatgctctt tttgcgcttt cgtccagccg ccgaactctt
     2761 catcaatggt gaataacttc agctttggaa acgcattttc gtactttttc gctacttcag
     2821 cgtcgcgcgg acggtagtag tttttcgcgg caatttcctg accttctggc gagtagagat
     2881 atttcaggta ggcttccgcc acctctttgg tgcctttttt ctcgaccact ttatcgacca
     2941 ccgacacggt tggctctgcg aggatagact cactcggcgt gacgatttcg aatttatctt
     3001 tccccagttc attcgctgcc agcagagctt cgttttccca ggcgatcagt acatcgccaa
     3061 tcccgcgctc gacaaaggtg ttagtggagc cgcgcgcgcc agaatccaga acttcgacgt
     3121 ttttatacag tgcccgaaca aaatcctgtg cttttgcctg atcgttgttg ttgtgatgca
     3181 gcgcgtagcc ccaggcagcc aggtagttcc agcgtgcgcc accagagctt ttcggattag
     3241 gcgtgatcac cgaaacaccc ggtttaatca gatcgttcca gtcatggatc tgcttcggat
     3301 tgcccttacg caccaggaaa acaatggtgg aagtgtacgg tgcggagtta tccggcagac
     3361 gtttgatcca ctctttatca atccgcccgc gttccgcaat tgcgtccacg tcataggcca
     3421 gagccagcgt gacaacatca gcttcaatac cgttgattac cgacgtcgct tgtttacctg
     3481 aaccaccgtg tgactgacgc atcaccacgt tatcaccagt ttgctgtttc cagtgggcgc
     3541 tgaatgcctt gttgtactgt tcgtacaatt cgcgcgttgg atcatatgaa acgttaagaa
     3601 gctgaatatc ctttgccata acgctggttg ccgccagcaa aaatgttaac cctacgcccc
     3661 acttgttcat cgcccgactc tcttatgttg tgttgtgatg agcaaagcgt gccagaaggt
     3721 taaccaaaca ttaaagaata aaaaaagatt ggctataact tgcgggtata tgttgaggga
     3781 ttaaaaaggc ggaagaaatc cgcctcatat tgctgacaaa gtgcgctttg tccatgccgg
     3841 atgcggcgcg aacgccttat ccggcctaca aaagtttgca aattcaataa attgcagaat
     3901 tcatgtaggc ctgataagcg aagcgcatca ggcatttttg cttctgtcat cggtttcagg
     3961 ctaaaggaat ctgccttttt ccgaaatcat taatacagtt ttttcgcgca gtccagccag
     4021 tcacctttga acggacgctt catgttttcg atagcgtcga tgatgtcgtg gtgaaccagc
     4081 tgttcgttct ggatacctac acaacgaccg ccgtaacctg ccagcagcag atcgatagcg
     4141 taagcgccca tacgggaagc cagaatacgg tcgtaaggca ccggagaacc accgcgctgg
     4201 atgtggccca gcacagttgc gcgggtttca cgaccggttt ctttctcgat gaaatgcgcc
     4261 agttcgtcaa catcacacat atgttcggta atcgccacga tcgcgtgttt tttacctttc
     4321 gcgatacccg ctttgatttc gtttaccagg tcttcacggc tgaattcaac ttccggaacc
     4381 acaacgaatt cacagccacc ggcaatggcc gcagccaacg tcagatcgcc acaataacgg
     4441 cccatcactt ccaccacgga aatacgctgg tgagaagaag aggtgtcacg cagacggtcg
     4501 atcgcttcta caacggtgct cagcgcagtg aagaaaccga tagtgtagtc agtgcctttg
     4561 atgtcgttgt cgatagtgcc cggcagaccg atgcacggga agcccatttc ggtcagacgc
     4621 attgcaccca tgtaggaacc gtcaccgccg ataaccacca gcgcgtcgat accacgtttt
     4681 ttcaggtttt cgatagccac ggcgcggatg ttctcgtcgc ggaattccgg gaaacgcgca
     4741 gaaccgagga acgtaccgcc acggttgatc atgtcagaca cgctgtaacg gtctagctgt
     4801 accatacggt cttcatacag acccagatag ccgtcataaa tacccattac ttccagacct
     4861 tctgtcagcg cagaacgaac aaccccgcga attgcggcgt tcatgcctgg cgcatcaccg
     4921 ccgcttgtca acacaccgat tttcttaatc atgactacct ctgaactttg gaatgcaaaa
     4981 tgaaatctgt tgccggaagt cttcttgcac atcgcagtga tccaacgaat gtgcaaatag
     5041 tataacaatc acttcctgct gaattgattc aggtcaggcc aaatggcggt attttataca
     5101 caaaatgcgg gtctggctct cttttatact gattatgaaa gcatagaccg tttaccctcc
     5161 ctgggtacga cggaacaggg gtcctgatgg ataattacat ccgatcccgg aaaacgccgt
     5221 aaaatagcct gctctacctg atccgccacc atatgtgcct gaaccaaagg cagagagtct
     5281 tccatttcca aatgaatctg aataaagcgg gtcggccctg actgccgcgt gcgaagatcg
     5341 tgagcgccgc taacacccgg ccaggaagtc acgatatcaa taatttcttg ccgttcctca
     5401 tcaggcaatg cgcgatccag taatgactgt accgcctcat atcccatgcg taacgcgcta
     5461 tataaaatat agatgccgat tcccaatgca aacagagcat cggcgcgatg ccagccgtac
     5521 caggacaacc ccagcgccag cagaattgcg ccgttcatca taacatcaga ctggtaatgt
     5581 agcatatcag cccgcaccgc ctggctttgc gtccggcgca ccacccaacg ctgaaacgag
     5641 acaaggataa tcgtacaaat tagcgccaca attgtcacga taaccccgac gcctggatct
     5701 atcatcggtg ttggagatat catatgttga atacccgtca aaaacaggaa tagtgccgaa
     5761 ccggagataa acatactttg cgccagcgcc gcgagggact ctgctttacc gtgaccaaac
     5821 gagtgattat cgtcggcagg ttgcagggaa tatcgcacca ccagtaaatt cgtcaacgac
     5881 gcgccgatat ccaccagcga atccaccagc gcggcgagaa tactcaccga cccggtatac
     5941 caccatgcaa aaattttaat cagcaatagc aacgaagcca tcgccgtcgc agcaatcgcc
     6001 gcccgactga ccagccgtcc ataagattga ttcataaata ctcccgctat caactgacgc
     6061 tagtataacg gaagcaaatc atctgcaatg cattaagcag caggcaaatt gaggataaaa
     6121 aaaaccccac atcatgtggg ggaagacagg gatggtgtct atggcaagga aaacagggtt
     6181 tactactggg aacgtgagtt gctactactc aatagcttca acgatgaact tttttgccat
     6241 tgcgtcacgt cacgcaactg ctccattcgt tgttgatgtt tctcgtttaa aaccgcttgc
     6301 tgctccggcg ttaacaggcg atacatttgg ttgcggactt ttgccatctc aacctgacga
     6361 gcaatttgct cattcgccat tttttctgcc tgtgcgcgca cagcgttttc atcaaaattt
     6421 tctgcggtga caaggcgatg cattgtctcc agttcgctaa cattaacagg aggctgttcg
     6481 tgccgggcct gttgcataag atctcgcatc tgctgacgct gatgttcggt taaacttatg
     6541 ccgtcgaaca tatggctctg cgtactgcgc tgcgtactgc gctgcgtact gcgctgcgta
     6601 agttcttcac ccggatgcca gttatcgtct gaaccgactt cagcagcgtg gcttaatgaa
     6661 ctgactgcca gcgttgaggc catgacggca gcggtaacta tgcgcatcat ttgctcccaa
     6721 aatctttctg tcgcgattca acgatagaga gtttacgatt caggctgcaa acatgcgtca
     6781 gggggtgtaa aacaacgtaa agtcatggat tagcgacgtc tgatgacgta atttctgcct
     6841 cggaggtatt taaacaatga ataaaatcct gttagttgat gatgaccgag agctgacttc
     6901 cctattaaag gagctgctcg agatggaagg cttcaacgtg attgttgccc acgatgggga
     6961 acaggcgctt gatcttctgg acgacagcat tgatttactt ttgcttgacg taatgatgcc
     7021 gaagaaaaat ggtatcgaca cattaaaagc acttcgccag acacaccaga cgcctgtcat
     7081 tatgttgacg gcgcgcggca gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga
     7141 tgactatctc ccgaaaccgt ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct
     7201 gcgccgttcg cactggagcg agcaacagca aaacaacgac aacggttcac cgacactgga
     7261 agttgatgcc ttagtgctga atccaggccg tcaggaagcc agcttcgacg ggcaaacgct
     7321 ggagttaacc ggtactgagt ttaccctgct ctatttgctg gcacagcatc tgggtcaggt
     7381 ggtttcccgt gaacatttaa gccaggaagt gttgggcaaa cgcctgacgc ctttcgaccg
     7441 cgctattgat atgcacattt ccaacctgcg tcgtaaactg ccggatcgta aagatggtca
     7501 cccgtggttt aaaaccttgc gtggtcgcgg ctatctgatg gtttctgctt catgataggc
     7561 agcttaaccg cgcgcatctt cgccatcttc tggctgacgc tggcgctggt gttgatgttg
     7621 gttttgatgt tacccaagct cgattcacgc cagatgaccg agcttctgga tagcgaacag
     7681 cgtcaggggc tgatgattga gcagcatgtc gaagcggagc tggcgaacga tccgcccaac
     7741 gatttaatgt ggtggcggcg tctgttccgg gcgattgata agtgggcacc gccaggacag
     7801 cgtttgttat tggtgaccac cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag
     7861 atcattcgta actttattgg tcaggccgat aacgccgatc atccgcagaa gaaaaagtat
     7921 ggccgcgtgg aactggtcgg tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt
     7981 tatctgattc gtccggccag cagttctcaa tccgatttca ttaacttact gtttgaccgc
     8041 ccgctattac tgctgattgt caccatgttg gtcagtacgc cgctgctgtt gtggttggcc
     8101 tggagtctgg caaaaccggc gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga
     8161 aacttacgcc agcacccgga actggaagcg gggccacagg aattccttgc cgcaggtgcc
     8221 agttttaacc agatggtcac cgcgctggag cgcatgatga cctctcagca gcgtctgctt
     8281 tctgatatct ctcacgagct gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta
     8341 ctgcgccgtc gtagcggtga aagcaaggaa ctggagcgta ttgaaaccga agcgcaacgt
     8401 ctggacagca tgatcaacga tctgttggtg atgtcacgta atcagcaaaa aaacgcgctg
     8461 gttagcgaaa ccatcaaagc caaccagttg tggagtgaag tgctggataa cgcggcgttc
     8521 gaagccgagc aaatgggcaa gtcgttgaca gttaacttcc cgcctgggcc gtggccgctg
     8581 tacggcaatc cgaacgccct ggaaagtgcg ctggaaaaca ttgttcgtaa tgctctgcgt
     8641 tattcccata cgaagattga agtgggcttt gcggtagata aagacggtat caccattacg
     8701 gtggacgacg atggtcctgg cgttagcccg gaagatcgcg aacagatttt ccgtccgttc
     8761 tatcggaccg atgaagcacg cgatcgtgaa tctggcggta caggattggg gctggcgatt
     8821 gttgaaaccg ccattcagca gcatcgtggc tgggtgaagg cagaagacag cccgctgggc
     8881 ggtttacggc tggtgatttg gttgccgctg tataagcgga gttaaactcc gcatttgtag
     8941 gcaggataag gcgtttacgc cgcatccggc atttgagcag gatgcctgat gcgacgctga
     9001 tagcgtctta tcaggcctac actccggggg ttattttccc cacaaccggc gggaaaaatc
     9061 ttcgatcttg ccgctcagtc ggcgcttttg catcgtccgc gtccagcttt tcgataaccc
     9121 ggcggcggag agtaaacggt gatactgatc gtcatcaaac ggcatatgcc atgcgatggc
     9181 ggcagcctcc tgcacggtca catcgctgac acgggaaacc aactccagcg gcgcgtccgc
     9241 agacactttc cctggcgcta tcacgctata caaccacccc accttacctg tgttttgcat
     9301 caactgcgct atatcgctga tatcaaaatg gtaattgagt ttatagcaag gcgagcgcgg
     9361 ctggctgacc tgaattaatg cctctcccca gcggaaaata tcgcccatat aaacattact
     9421 ttccgtcagg ccgtcggttg agaggttttc accaaacgca ggcgcaacaa acaactccgc
     9481 ctgttccgga aattcccgcg cccagtagag ataatgctca cgaggataat gacacagcgc
     9541 tctgtctggc ccaccgtgaa cctttttctc cgcctgctcg tcaccttcca gccccagctc
     9601 cgtcagcatc aactcaccat caacctggat tttggcaatt gcgctgggtt tgccttcggg
     9661 ataagcctga atcttgcctg tgtatacatc aaccggatat cgcatctgtt ccctctccac
     9721 gagaaatttc cccattagac catatttatt ttatgtcagg cgtgctgttt tcgcggtaag
     9781 tatccaggca ggaactcaat agcaaaccgg acgaacgtta gttcatccgg ctgtggggat
     9841 taaggaagca ggtttatttc accgtaccta aaatttcgat tttcgctgct ctggctttga
     9901 ctttacgtga ccagatagag gtaagaattg gcaccagaat cgaagtcaca atgaccgccg
     9961 tcgctaccag cgaagttgct gccggagcca tcggtttaaa cgcaggcacc atttctgcaa
    10021 tcagcacagg tgtcgctacc gcggcccctg cggaactgga agcggcaatt ccggcagtgc
    10081 catcgccacc gccaatcaat ttatcggcga taatcaacgg aataccggtc acgataatta
    10141 ctgccacacc caacaggatc cccagcaaac cagtctgggc aatcacagtc aaatcaatgg
    10201 tattgcccag cgcgaaggca aagaatggaa tcagcgtttg caccgctttg ctgaaaaatt
    10261 ctcgtaattc agggtcaagg ttccccaggg caaagcccac gaggaacggt aatactgcgc
    10321 cgacgaaaac atgcggttca aacgaggcaa tccctgcagt gcccagaata atcatcgtca
    10381 tgagcgcccc ggactccaac gacatcaaca caaatgcccc agcttcttct tttgtgccgt
    10441 actgctgcat gatggaagcg taaagtccgc cattggtcat atccatcgcc gccaccagcg
    10501 ccagcgttga aagtccggca aagaatccaa cttcaacgcc atgttccgga ataatgcgcg
    10561 aggcaattgc cgcaaccacc cacgcgacgg caatttttgt taccaccagc gtaccggatt
    10621 tacgcaatac cgttcccgtc gcgcttaatt ttattgacgc ccccatgcaa aaaaaccaca
    10681 ccgccagaat ggacaccgta ccggtaatca tcccgttggt gaatgatcca aaatatttcc
    10741 ccgcccccgg cgagaaggtg tggcacagtg cgccaaggaa tagcgggacg agcatcatcc
    10801 ccccgggatt ttctcaatcg agcgttttat ctgcatttcc atcacctgtc ggcatatttt
    10861 taagggctga tgcgtaattc ctcaatggag catcagtgtt gatggaaacg aagatagaca
    10921 ttcatttaat caataaaagt gatcaaaacc acattaattg aaacgctgtt ttatttttat
    10981 cggatcattg tttttatttt gaaattgaac acaaaaaaac caccgccgtt ggcgatggtt
    11041 cattttttaa gctgatatgc ggcgtaaacg cctcattgca gcaggcggca aatgattatt
    11101 ttttcgccgc gaaacgtgcc gctgcttcat cccagttcac cacgttccag aactctttga
    11161 tgtagtccgg acggcggttc tggaatttca ggtagtaagc atgttcccac acatccaggc
    11221 ccagaatcgg gaagccggaa gcgccagaaa tagcttcacc catcagcggg gaatcctggt
    11281 tagcagtaga aaccaccgcc agtttatcgc ctttcagcac cagccatgcc cagccggaac
    11341 caaagcggga agctgccgct ttctcaaatt ctgctttgaa gttatcaacg gagccgaagt
    11401 cacgttcgat agccgctttc aggtcaccct gtaaggtggt gccttttttc agacctttcc
    11461 agaacaggct gtggttagcg tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg
    11521 ctggcagctg gtccagtttg gtgatcagct cttcaaccgg caggttggca aattctggca
    11581 ggctttccag cgccgcgttg gcgttgttga cgtaggtctg atggtgtttg gtgtggtgga
    11641 tttccatggt ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg
    11701 gcagggtata gctcatattc atctccagta ttgtcgggcg gccgattgtt aatgcgcgta
    11761 agcagttggt tcattatagt taattaaatg atattgaaaa tgattatcaa tgctgtactt
    11821 ttcgtaaggg tatggttttg caggaaaatg cccgagatgt gaagcaaatc acccacttaa
    11881 tgccgtgatt gccagtaaat cgacaacggc ggcaacaggc gaaaggttaa tcgacagcac
    11941 gatttttaca ctcatctcgt cggagatgtg acgcgacgaa aaatgatgag gataagaaga
    12001 tgagtaacgc gattacgatg gggatatttt ggcatttgat cggcgcggcc agtgcagcct
    12061 gtttttacgc tccgttcaaa aaagtaaaaa aatggtcatg ggaaaccatg tggtcagttg
    12121 gtgggattgt ttcgtggatt attctgccgt gggccatcag cgccctgtta ctgccgaatt
    12181 tctgggcgta ttacagctcg tttagtctct ctacgctgct gcctgttttt ctgttcggcg
    12241 ctatgtgggg gatcggtaat atcaactacg gcctgaccat gcgttatctc ggcatgtcga
    12301 tgggaattgg catcgccatt ggcattacgt tgattgtcgg tacgctgatg acgccaatta
    12361 tcaacggcaa tttcgatgtt ctgcttaata ccgaaggcgg acgcatgacg ttgctcggcg
    12421 ttctggtggc gctgattggt gtagggattg tgactcgcgc cgggcagttg aaagagcgca
    12481 aaatgggcat taaagccgaa gagttcaacc tgaaaaaagg gctggtgctg gcggtgatgt
    12541 gcggcatttt ctctgccggt atgtcttttg cgatgaacgc cgcaaaaccg atgcatgaag
    12601 ccgctgccgc actgggcgtc gatccactgt atgtcgctct gccaagctat gtcatcatca
    12661 tgggcggcgg cgcgatcatc aacctcggtt tctgcttcat tcgtctggca aaagtgaagg
    12721 atttgtcgct aaaagccgac ttctcgctgg caaaaccgct gatcattcac aatgtgttac
    12781 tctcagcact gggcgggttg atgtggtatc tgcaattctt tttctatgcc tggggccacg
    12841 cccgcattcc ggcgcagtat gactacatca gttggatgct gcatatgagt ttctatgtat
    12901 tgtgcggcgg tatcgtcggg ctggtgctga aagagtggaa caatgcaggt cgccgtccgg
    12961 taacggtgtt gagcctcggt tgtgtggtga ttattgtcgc cgccaacatc gtcggcatcg
    13021 gcatggcgaa ttaatctttc tgcgaattga gatgacgcca ctggctgggc gtcatcccgg
    13081 tttcccgggt aaacaccacc gaaaaatagt tactatcttc aaagccacat tcggtcgaaa
    13141 tatcactgat taacaggcgg ctatgctgga gaagatattg cgcatgacac actctgacct
    13201 gccgcagata ttgattgatg gtcattccag tctgctggcg aaattgctga cgcaaaacgc
    13261 gctcactgca cgatgcctca tcacaaaatt tatccagcgc aaagggactt ttcaggctag
    13321 ccgccagccg ggtaatcagc ttatccagca acgtttcgct ggatgttggc ggcaacgaat
    13381 cactggtgta acgatggcga ttcagcaaca tcaccaactg cccgaacagc aactcagcca
    13441 tttcgttagc aaacgacaca tgctgactac tttcatgctc aagctgaccg ataacctgcc
    13501 acgcctgcgc catccccacg ctacctaagc gccagtgtgg ttgccctgcg ctggcgctaa
    13561 atcccggaat cgctccctgc cagtcaagat tcagcttcag ccgctccggg caataaataa
    13621 tattctgcaa aaccagatcg ttaacggaag cgtaggagtg tttatcgtca gcatgaatgt
    13681 aaaagagatc gccacgggta atgcgataag ggcgatcgtt gagtacatgc agaccattac
    13741 cgcgccagac aatcaccagc tcacaaaaat catgtgtatg ttcggcaaaa acatcttgcg
    13801 gataacggtc agccacagcg actgcctgct ggtcgctggc aaaaaaatca tctttgagaa
    13861 gttttaactg atgcgccacc gtggctacct cggccagaga acgaagttga ttattcgcaa
    13921 tatggcgtac aaatacgttg agaagattcg cgttattgca gaaagccatc ccgtccctgg
    13981 cgaatatcac gcggtgacca gttaaactct cggcgaaaaa gcgtcgaaaa gtggttactg
    14041 tcgctgaatc cgcagcgata ggcgatgtca gtaacgctgg cctcgctgtg acgtagcaga
    14101 tgtcgggcct tcatcagtcg caggcggttc aggtaacgct gaggcgtcag tcccgtttgc
    14161 tgcttaagct gccgatgtag cgtacgcagt gaaagagaaa attgatccgc cactgcatcc
    14221 caattcacct catcggcaaa atggtcctcc agccaggcca gaagcaagtt gagacgtgat
    14281 gcgctgtttt ccaggttctc ctgcaaactg cttttacgca gcaagagcag taattgcata
    14341 aacaagatct cgcgactggc ggtcgagggt aaatcatttt ccccttcctg ctgttccatc
    14401 tgtgcaacca gctgtcgcac ctgctgcaat acgctgtggt taacgcgcca gtgagacgga
    14461 tactgcccat ccagttcttg tggcagcaac tgattcagcc cggcgagaaa ctgaaatcga
    14521 tccggcgagc gatacagcac attggtcaga cacagattat cggtatgttc atacagatgc
    14581 cgatcatgat cgcgtacgaa acagaccgtg ccaccggtga tggtataggg ctgcccatta
    14641 aacacatgaa tacccgtgcc atgttcgaca atcacaattt catgaaaatc atgatgatgt
    14701 tcaggaaaat ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt accagacgga
    14761 aaaaaatcca cactatgtaa tacggtcata ctggcctcct gatgtcgtca acacggcgaa
    14821 atagtaatca cgaggtcagg ttcttacctt aaattttcga cggaaaacca cgtaaaaaac
    14881 gtctattttt caagatacag cgtgaatttt caggaaatgc ggtgagcatc acatcaccac
    14941 aatgaagcaa attgtgaaca tcatcacgtt catctttccc tggttgccaa tggcccattt
    15001 tcctgtcagt aacgagaagg tcacgaattc aggcgctttt tagactggtc gtaatgaaat
    15061 tcagcaggat cacattatga cctttcgcaa ttgtgtcgcc gtcgatctcg gcgcatccag
    15121 tgggcgcgtg atgctggcgc gttacgagcg tgaatgccgc agcatgacgc tgcgcgaaat
    15181 ccatcgtttt aacaatgacc tgcatagcca gaacggttat gtcacctgga atgtggatag
    15241 cctggaaagt gccattcgcc ttggattaaa caaggtgtgc gaggaaggga ttcgtatcga
    15301 tagcattggg attgatacct ggggcgtgga ctttgtgctg ctcgaccaac agggtcagcg
    15361 tgtgggcctg cccgttgctt atcgcgatag ccgctccaat ggcctaatgg cgcaggcaca
    15421 gcaacaactc ggcaaacgcg atatttatca acgtagcggc atccagtttc tgcccttcaa
    15481 tacgctttat caactgcgtg cgctgacgga gcaacaacct gaacttattc cacacattgc
    15541 tcacgctctg ctgatgccgg attacttcag ctatcgcctg accggcaaga tgaactggga
    15601 atataccaat gccaccacca cacaactggt caatatcaat agcgacgact gggacgagtc
    15661 gctactggcg tggagcgggg ccaacaaagc ctggtttggt cgcccgacgc atccgggtaa
    15721 tgtcattggg cactggattt gcccgcaggg taatgagatt ccggtggtcg ccgttgccag
    15781 ccatgatacc gccagcgcgg ttatcgcctc gccgttaaac ggttcacgcg ctgcttatct
    15841 ctcttctggc acctggtcat tgatgggctt cgaaagccag acgccattta ccaatgacac
    15901 ggcgctggca gccaacataa ccaatgaagg cggggcggaa ggtcgctatc gggtgctgaa
    15961 aaatattatg ggcttatggc tgcttcagcg agtgcttcag gagcggcaaa tcaacgatct
    16021 cccggcgctt atcgccgcga cacaggcact tccggcctgc cgcttcatca tcaatcccaa
    16081 tgacgatcgc tttattaatc ctgaggcgat gtgcagcgaa attcaggctg cgtgtcggga
    16141 aacggcgcaa ccgatcccgg aaagtgatgc tgaactggcg cagctacgcg gtgaagattt
    16201 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgtgc
    16261 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcgacgc tcggcaatat
    16321 cggcatccag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
    16381 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
    16441 tgtggcgcag cttcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
    16501 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
    16561 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
    16621 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
    16681 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
    16741 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
    16801 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
    16861 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
    16921 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
    16981 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
    17041 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
    17101 gcagcactgg atgaggtgat cagcaagaag ctaaaccctg cgcaccatat cgacgccgtt
    17161 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
    17221 atggggtatg ccaccagccg ccagactgcg ctgtgcctag acgccgggca cttccacccg
    17281 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtgccgca gttgctgctg
    17341 cacgtcagcc gtccggttcg ctgggactgc gatcacgtag tgctgctgga tgatgaaacc
    17401 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
    17461 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
    17521 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
    17581 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgtggcag
    17641 gcggtctggg aaatgtattg ccaacgtcac gatacgccag caggtagcga atggctggag
    17701 agcgtgcggg cttatgaaaa agaaattttg agccagcgtg ggtaaacact gccggatgcg
    17761 gcgcgagcgc cttatccggc ctacgggtcg gcaacagttg taggcctaat aagacgcgac
    17821 agcgtcgcat caggcattga ttgccggatg cggcacgagt gccttatccg gcctacaggt
    17881 cggcaatagt tgtaggcctg ataagacgcg acagcgtcgc atcaggcatt gattgccgga
    17941 tgcggcacga gcgccttatc cggcctacgg gtcgtgcatc tgacaacacc gaatttacag
    18001 gaacacagaa catgcaaaac attacccagt cctggtttgt ccagggaatg atcaaagcca
    18061 ccaccgatgc ctggctgaaa ggctgggacg aacgcaacgg cggcaacctg acgctgcgcc
    18121 tagatgacgc cgatatcgcg ccttataaag acaacgtcca tgcacaaccg cgctatatcc
    18181 cgctcagcca gcccatgcct ttactggcaa atacaccgtt tattgtcacc ggctccggca
    18241 aattcttccg taacgtccag ctcgatcctg cagctaactt aggcgtcgta aaagtcgaca
    18301 gcgatggcgc gggctaccat attctctggg ggttaaccaa cgaagccatc ccgacatccg
    18361 aactcccggc gcactttctc tcccactgcg agcgcattaa agccaccaac ggtaaagatc
    18421 gggtgatcat gcactgccac gccaccaacc tgatcgccct cacctatgta ctggaaaacg
    18481 acaccgcggt ctttactcgc caactgtggg aaggcagcac cgagtgtctg gtggtattcc
    18541 cggatggcgt tggcattctg ccgtggatgg tgcccggcac ggacgaaatc ggccaggcga
    18601 ccgcacaaga gatgcaaaaa cattcgctgg tgttgtggcc cttccacggc gtcttcggca
    18661 gcgggtcgac gttggatgaa acattcggtt taatcgacac cgcagaaaaa tcagcacaag
    18721 tattagtgaa ggtttattcg atgggcggca tgaaacagac catcagccgt gaagagttga
    18781 tagcgctcgg caagcgcttc ggcgttacac cactagccag tgcgctggcg ctgtaaggag
    18841 caaacatgat ccgcaaagcc tttgtcatgc aggttaaccc cgacgcccac gaagagtatc
    18901 agcgtcggca taaccccatc tggccggaac tggaagcagt gttgaaatct cacggtgcgc
    18961 ataactacgc catttacctc gacaaagcgc gtaatctgct gtttgccacg gtagagattg
    19021 aatctgaaga acggtggaat gcggtagcca gtactgatgt ttgccaacgt tggtggaaat
    19081 atatgaccga tgttatgccc gctaacccgg ataacagccc ggtgagtagc gagctg
//
LOCUS       DAJTNA010000069        19100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTNA010000069 DAJTNA010000000
VERSION     DAJTNA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19100
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.070"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            40..969
                     /gene="aaeR"
                     /locus_tag="OO161_002044"
     CDS             40..969
                     /gene="aaeR"
                     /locus_tag="OO161_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5463112.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1097..2542)
                     /gene="tldD"
                     /locus_tag="OO161_002045"
     CDS             complement(1097..2542)
                     /gene="tldD"
                     /locus_tag="OO161_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5463113.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADTWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2698..6498)
                     /gene="yhdP"
                     /locus_tag="OO161_002046"
     CDS             complement(2698..6498)
                     /gene="yhdP"
                     /locus_tag="OO161_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5463114.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRGPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGDVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRDWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6566..8035)
                     /gene="rng"
                     /locus_tag="OO161_002047"
     CDS             complement(6566..8035)
                     /gene="rng"
                     /locus_tag="OO161_002047"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5463115.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8025..8618)
                     /gene="yhdE"
                     /locus_tag="OO161_002048"
     CDS             complement(8025..8618)
                     /gene="yhdE"
                     /locus_tag="OO161_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5463116.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8627..9115)
                     /gene="mreD"
                     /locus_tag="OO161_002049"
     CDS             complement(8627..9115)
                     /gene="mreD"
                     /locus_tag="OO161_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5463117.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9115..10200)
                     /gene="mreC"
                     /locus_tag="OO161_002050"
     CDS             complement(9115..10200)
                     /gene="mreC"
                     /locus_tag="OO161_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5463118.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRTLRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPHSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10266..11309)
                     /gene="mreB"
                     /locus_tag="OO161_002051"
     CDS             complement(10266..11309)
                     /gene="mreB"
                     /locus_tag="OO161_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5463119.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11614..13554)
                     /gene="csrD"
                     /locus_tag="OO161_002052"
     CDS             complement(11614..13554)
                     /gene="csrD"
                     /locus_tag="OO161_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5463120.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAVYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSIQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13706..14680
                     /locus_tag="OO161_002053"
     CDS             13706..14680
                     /locus_tag="OO161_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5463121.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15658..16128
                     /gene="accB"
                     /locus_tag="OO161_002054"
                     /gene_synonym="fabE"
     CDS             15658..16128
                     /gene="accB"
                     /locus_tag="OO161_002054"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5463122.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16139..17488
                     /gene="accC"
                     /locus_tag="OO161_002055"
     CDS             16139..17488
                     /gene="accC"
                     /locus_tag="OO161_002055"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5463123.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17580..18627)
                     /locus_tag="OO161_002056"
                     /pseudo
     CDS             complement(17580..18627)
                     /locus_tag="OO161_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(18624..>19100)
                     /locus_tag="OO161_002057"
     CDS             complement(18624..>19100)
                     /locus_tag="OO161_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5463124.1"
                     /translation="SFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHS
                     TPERAIAGFLEEGVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAW
                     IACRQLGFDVHRALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIQEAAQ"
BASE COUNT         4596 a         5273 c         4725 g         4506 t
ORIGIN      
        1 tattatgtga tctaaatcac ttttaagtca gagtgaataa tggaacgact aaaacgcatg
       61 tcggtgtttg ccaaagtagt tgaatttggc tcttttactg ccgccgccag acagctacag
      121 atgagcgttt cgtccatcag tcagacggtg tcaaaactgg aagatgagtt gcaggtaaag
      181 ctgttaaacc gtagcacacg cagcattggc ctgaccgaag ccggtagaat ttactaccag
      241 ggctgccgtc gtatgcttca tgaagtgcag gatgttcatg agcaactgta tgccttcaat
      301 aacaccccca tcgggacgct acgcattggc tgttcttcaa ctatggcaca aaatgttctc
      361 gccgggctga cagccaaaat gctgaaagaa tacccaggtt tgagcgtcaa tctggttacc
      421 ggaattccag cccccgacct gattgccgac ggtctggatg tggtgatccg cgtcggcgcg
      481 ttgcaggatt ccagcctgtt ttcccgccgt cttggcgcga tgccaatggt ggtgtgcgcc
      541 gcgaaaagct atctcacaca atacggcata ccggaaaaac ccgccgattt aagtagccat
      601 tcatggcttg aatacagcgt gcggcccgac aatgaatttg aactgattgc accggaaggg
      661 atctcgactc gcctgatccc gcaagggaga tttgtgacta atgatccgat gacgctggtg
      721 cgctggctaa cggcgggtgc cgggatcgcc tacgtgccgc tgatgtgggt gatcaacgag
      781 atcaatcgtg gggagctgga gatcctgctg ccgcgttatc agtcagatcc acgcccggtt
      841 tatgcgttat ataccgaaaa agataagctg ccgctgaagg tacaggtcgt gatcaactcg
      901 ctgacggatt attttgttga ggtcggtaaa ttgtttcagg agatgcacgg gcgcgggaaa
      961 gagaagtaat tcatattgta ctgttacgtt ctacaaacct gtgccaacgg gttcccctca
     1021 ccctaacccc agaggagcga ggggaccgtt cgtgcacgta gaaagattaa ttatccttct
     1081 gaaaataatg aaattattac gcagtaccgc caacagtcag gttatcaact ttcagcgttg
     1141 gctggcccac gccaaccggc aaactttgcc cttctttacc gcagacaccc acgccgttat
     1201 ccagtttcag gtcgttgcca accatcgaaa tctgctgcat ggtttcgata ccggaaccaa
     1261 tcaacgttgc gcctctcacc ggcttcgtta ctttaccgtt ttcaatcaga tatgcttctg
     1321 aagtggagaa aacgaatttg ccggaggtga tatccacctg accgccacca aagttcggtg
     1381 catagatacc gtactcaacg gattcaataa tttcctgcgg ggtcgattta cccggcagca
     1441 tataggtgtt ggtcatacgc ggcatgggca gatgggcgta ggattcacgg cgaccgttgc
     1501 cagtcggcgt catccccatc aaacgcgcgt tgagtttatc ctgcatgtag cctttcagaa
     1561 tgccgttctc aatcagcacg ttgtactggc ctggcgtacc ttcgtcatca atcgccaccg
     1621 aaccacggcg atcgaccatc gtgccgtcat caaccacggt acacagttct gaagccacca
     1681 gctccccgac ctgcccacta aatactgaag tgccgcggcg gttgaagtcg ccttccagac
     1741 cgtgacctac cgcttcatgc aacagcacgc ccggccaacc tgcgccaagt actaccggca
     1801 tggtgcccgc tggtgcagca acggcagaaa gattgaccag cgccatacgc actgcttctt
     1861 ttgcccatgt atccgcacgg acttcgccgt caagatcggc aaggaagaac tcataaccaa
     1921 aacgaccgcc accgccactg gcaccgcgtt cgcgtttgcc atcttcttcg accagaacgc
     1981 tcacggaaag acgcaccagc ggacggacat ccgccgctag cgtgccgtcg gtggccgcaa
     2041 ccagaattaa ttcatagaca ccactgaggc tggcagtcac ttcctgtacg cgcttgtccg
     2101 cttcgcgagc aaccttatcg acgcgacgca ggatatccag cttctcttca cggctcatgc
     2161 tttgcagcgg atctaccgag gtatacaacg ggctatgccc taccgcgccc agcgtctgta
     2221 ctttgccatc accactatca cggacgatgg tgcgcgccgc ttgcgcactc tgttccagcg
     2281 ccagcaggct gatttggtca gcgtaagcaa atccggtttt ttcaccgctg attgcacgca
     2341 cgccaacgcc ctgatcgatg ttgtaagaac catctttaat aatgcggtct tctaaaaccc
     2401 aggattcgtg atagctcgac tgaaaataga gatcgccata atcaaggcga cgttcggcca
     2461 gttgaccgag gatcgcgaac aagtcctgat gtttcaggcc gttcgccgct agcaattgtt
     2521 cacttaccag gttaagactc atcgtttttg ctactcgtta gttactgcag tagaggattt
     2581 tttacggctg ccggatgcgg cgtgaacgcc ctatccgacc tacggttatg ttctggctcg
     2641 ttggcctttg gcaacgatta tcatatgaga ttggggcaat tacgcgccct cgtcaaatca
     2701 ttgcgctttt tctttacgcg gttggcgcaa cacttcgttg atttgcggat cgtccagcgg
     2761 acccgaaatg tgatagcgca aaatggagac tttgctccac agcggcccca gcactttact
     2821 ggctgcaaat actgccgcgc caacaatggg gttaaccgca aaagccgcag ccacgcccac
     2881 cgtcgcagaa atctctggtg cgacaaccgc ttccatattc aggtcgcgac gtaccagatt
     2941 taccgaccct ttcatggcga tatccgcctc caggccatcc accagcgtgt cgtcggtgtg
     3001 cataacgccg tctttaatcc acgcggtgct gcgaatggag tcaaaataga acccttcgcc
     3061 aaaagtgtct ctgaaatcaa aacgcagctt acgcatcagg gcatcaacgc tcaataagcg
     3121 cagcaattgc ccggcatgtc cggtattgat ttcggtaatt tcgcctttac ccagttgagt
     3181 atgaatgatg ccattcaacg tcgcctcatc tggttgccag ggtgctttgc gccagtgtaa
     3241 atcgtaatcc acattaaatg acgactggcg tatgggcgtc gtgacaccaa aaaattctgc
     3301 ggcggcatca attttctgcc cgcgcagttt tcctttcagc gaggtacgtt cattccccgg
     3361 attattaacc cattcaccat cggcagtaag ccgcgagaaa ccagtatcaa tcagtccatt
     3421 ggtcagcgtt aacgtatcgc cagaaatggt gagatcactg tcaatacgac cgaatttttg
     3481 cccccagaac cagcactctg tgcatcgtat ttgtgcgtcc ggccagtcac ggaagttaat
     3541 gcgctccgtt gtcgggaaag gtgatgacgg tgttgaatca ccacgagttt tcgccacgct
     3601 cgggttgtaa taaagatatt tgatattcgc cagccacggc gcgttattac gcatcgccag
     3661 cgtggcattg atttcacgcc cctgcgcctc aaccagggtg ccatttgccg tcggttgcga
     3721 aacaatactc aggttattcc attgctgatt tcccagtgac aacataggcg tacgtaacgt
     3781 tatgtgttgt gggaaactcg ctgcaccacc gacactctcc gcagcgcctt tctggaacag
     3841 ggccagccac tcggcaccat tcatcggcgg catattgagt tcaacgccac tttgttccgg
     3901 caacggcggg agcgttttac tgtctgccgc ccaaatagca cgatcgagcg tcagcttttg
     3961 accaagcaac cagcggctat tgaaatggtt atccgcacca gcctgtccgg ttaattcaaa
     4021 gctgctgaga ttgccatcaa ccttaacgtt taccggcagt ggttcacccg caggtttggc
     4081 taacggtgaa ggtaagtgac tgctcacatt attcagatcg ccgttcagct ctacgttata
     4141 ggtcgcgcca gcatgataag gcagatcaat gcccacttta ccatcccacg ccacgctgcc
     4201 actcaatgct tcgttcaccg ctgcaggcag aacgccggtt ttcgccggtt gccagttacc
     4261 gttgaggttt accgcaacct ggtaggcttt tgccccttct ttggtggaaa aatccacgtt
     4321 caacggctga ttaaaccagc ttgctgtcag tggttcactt tgcagatcgc cattgataaa
     4381 gctgaattta ccgctcaaat ttttcagggt gctgtcgagt ggtttgataa acagactgtt
     4441 attacgcagc gtcacttcac ctttcgcggt taccagttcg ccgttcagcg ggatatcaag
     4501 atgtaagcga gcattcacat cgccgtcgag ctggagttct tgcagggtcg cacccagaga
     4561 atctttcagc ggtgtctcat caaagtaagg gccaacggct ttacccggac ctttaatgtc
     4621 agcgtcaatc agcagttttt ctttcgagta gtcagggatc actgcggtga gattactcgc
     4681 gcgcacgtcg cccagattaa cgtcatcggt tttcatccat aaaccgtcgt taataaagtc
     4741 cagttcaata tcaaggttag ttaatgcagg ccagtccggc tggaaggcaa acttcgcgtt
     4801 acgcagcggc accagcactt caaactgacc ttcgttgtgt ttatagggga agagttgcgg
     4861 attgccacca taaaccagcg tcgcgttatc cgcttcaccg ccctgaatcg cgccacttaa
     4921 gtaatcaact aggtctttac ccatcaagtt ttccgggaaa tagcgccagg cttgtgaacc
     4981 atcatcggta ctgatgccag ccagaatacc cagccagggt tcatcgttag caggttgcag
     5041 gtaacgaaaa ccgccgcgcg catggacggc tttggcttta acgtcaatat tacgcccatc
     5101 cagctggaaa cctttgtcat tgttcagcca gcttatagtt gcctggccgt cggcgatttc
     5161 cagtggccca cggaataccg tttcgtaagg catctttgcc tgcttcatcg acgcggtaag
     5221 caaaccattt tcaacgctgc cggaaagcgt cccggagaag tgttccgcac ccggtaataa
     5281 tttccattgc ttccaggcca gatcgctcca cgatgcctga aaacgggtct tgtctgccgc
     5341 ctgaagcggg atatccagcg ccagagtgtt aatcttgccg ctcggttgtg tggagcgcca
     5401 aacatcaccc agtgcaggtg aaagtttcgc ggccagcggg cgtatgccct ccaggcctgc
     5461 cagctccaga ttactggcgc gaatccggag ttcgtcactg cgtttattgt ctttgccgcc
     5521 aacgtcctgt tccggtatcc aggccagcgt caatgctccg ctcggccagg gtttgccgtc
     5581 catcgtgatc cgtgtatcgg gaatagagaa ctgccagccc ggattttcac gcgtaatatg
     5641 cgcggtcaga ttatccaccg acagcgtatg cgtttgcttc tcgcccaacc agctggcacc
     5701 gccctgtttc agccagacgt caccgccggt tacatcgcct ttgtcgatcg tcatccagcc
     5761 ttcaagggag aactgtgccg tttccagagc aatattgtcc tgcatccatt taccgagcca
     5821 cggcttcagg tcgatgtcat ccgcctgtag ccagacgcga ccattgctta acaacccctc
     5881 atcatcgcgc aaatccatgc gcacctgcat cacgccgtgc tgtccggtaa ggctggagag
     5941 gcttaccagg ccttccgcac ggtgtcgacg tggatcgttc agccaggtga gttgtgggat
     6001 cgccagctcg gcgcgctgac cggatggcgt cagaaaactg acttcactgt cgcggagatc
     6061 gaaatggtca aattgacgaa gaaacagatc gctgatgtga ctggcttcca ggctatcatt
     6121 accaccaccg ctggtgatag gagtgttggt gcgaaagcgc agctgccaga aagtgaggtc
     6181 gcgaaactgc cagcgcatat gtaacaagct ctgccagaca tccagcgcca gagtaacgcg
     6241 tttaaccgaa aattcgccgc catcttttag ttctgcacgg atgtcgtgtg cttcaagcgt
     6301 cgggccaaaa ttctgccagc tggctgagag ctgactggct tctaccggca tgccagtcgc
     6361 ggattctatt ttgttgagga tttccggacg ccaggcgtca agatgcggta aagcaatacg
     6421 caggccgcta accagcaggg ctgcgatcac aacgagcgcg gctccagtaa gcagtaaaat
     6481 ccccggcaat cgcctcaccc gtcactcctt gtctgctaaa aatgtgactc aaaaaacctt
     6541 tgccggatgg cggcccagca tctgtttaca tcattacgac gtcaaactgc tcctggttat
     6601 agagcggttc aatttgtact ttaacctgtt tgccaacgaa aatttccact tccgccagcg
     6661 agtgtgactc ttcgcctttc aaggcttcag ctactgccgg agaagcatag accaggaaac
     6721 ggtcggagtc gtaagcatgg tggacacgaa caatctcgcg catgatttca tagcataccg
     6781 tttccacggt tttcaccgtt ccgcgaccgt ggcaggttgg gcattcgtta cacagtacgt
     6841 gctcaatgct ttcgcgggtg cgtttacgcg tcatctccac cagccccagc gccgaaaaac
     6901 cattaacgct ggttttcacc cggtctttgc tcaacgcctg ctccagcgag tgcagcacgc
     6961 ggcggcggtg atcttcatta ttcatatcga tgaaatcaat aatgataatc ccacccagat
     7021 tacgcaaccg taactggcga gcgatagcct gcgtcgcttc aatattggta ttgaaaatgg
     7081 tgtcgtccag attgcgatga ccgacaaacg ctccggtatt gatgtccacg gtggtcatcg
     7141 cttcggtctg gtcgataatc agataaccac cggatttcag ttctactttg cgttccagcg
     7201 ctcgctggat ttcgttttcg acatcaaaga gatcgaaaat cggctggcgt ccggtgtaat
     7261 gctccagctt gctggtcatc tcgggaatgt actccgaggt aaactccagc aatgcttcgt
     7321 aagtcaggcg tgagtcaacg cgaatgcggt ccagttcggc gtcggcgaaa tcacgcagaa
     7381 cacgctgcgc cagcgccagt tcgccgtaca gctgataacg ggtctgcggg cgttttttac
     7441 gctccataac tttggtccag acgcgtttca gataagcggc atcggaggcc agttcagcct
     7501 cgccaacccc ttccgctgcg gtacggatga taaacccgcc ctgctcgtcg caatactctg
     7561 cgaccacttt tttcaggcgt tcacgttctg attcgctttc aatacgttgg gaaaccccaa
     7621 cgtgagaagc ccctggcata aacaccagat agcgagaagg cagcgtgata tcggtggtca
     7681 ggcgcgcacc tttagtgcca agcggatctt tcaccacctg caccatcaga tcttgcccct
     7741 gacgaaccag ttccgagatg tcgcgcaccg tgaattgctt ttgttcttca cccgccacac
     7801 attcggtgtg cggcatgata tcggatgcat gaagaaacgc ggctttatcc agcccaatat
     7861 ctacaaaagc cgcctgcata cccggaagta cacgacttac acgacccttg tagatattgc
     7921 ctactattcc gcgtcgcgcc tcacgttcaa tatgaatttc ctgcagaata ccgccatcaa
     7981 tatacgccac tcgcgtttcc gaaggcgtta cgtttactaa caattcagcc gtcatgttta
     8041 tcccttttct cacgcagtgc gttaaaatta cttaataatt catacgtttc aaccagcggt
     8101 aagccgacta cggcgtgata gctgccattt atcttcctga caaaacagcc tcccagcccc
     8161 tgaataccgt atgcacctgc tttatctaac ggttcaccgc tggcgacata gcccgcgatg
     8221 tcttcgtctg ttaacgttct gaaagtcaca tcggtgacca ccaggcaatc gagaatgtgc
     8281 tggctgtcgg ccaacaccac tgccgtcatc acctgatggg tctgacccga taatttgcgc
     8341 aacatctgcg ccgcatgctc tgcgtcgcgc ggtttctcca gcacttctcc attcaggata
     8401 acgatagtat ccgcacccag caccgggaga tcctgcgccg tttgcgcgac acccgcctgt
     8461 gctttctcgc gtgccagacg cacaacatac tgctgcgcgc tctcctgcgg ctgacgctgc
     8521 tcctcaatgc ccgtaacaat acgttcaaag gtcacgccaa gttgcgcaag taactcctga
     8581 cgacgcggag aaccggaagc taaatacaga gaagtcatag aaacctttat tgcactgcaa
     8641 actgctgacg gactttacgc atcagcaaga aaatccacgg ccagagcacc ccattgacta
     8701 cactactcca gaacacttcc ggtctgaaag agacgttaat cactaaaaac tctgcccaga
     8761 aaacaataat atccaccacc agcgaaagca acatgacgac tagcgcctgc tgccataatg
     8821 cgaggttgcg gaaaagctgg tatttcagcg ccaccaggta agcaatgatg ctcatcgcca
     8881 atacgcgtac gccaagcgtc gagccgctga tcagatccag tatggcaccc atcacaaaac
     8941 ctgtgcccac atttacgcga tggggcaagg ccaggatcca atacaacaag ataagtaaca
     9001 cccagtttgg ccggaaaaca atcaggttat ccggccaggg catgatttgc agcaacagcg
     9061 caatgaggaa agagagccag attacccaac gtccctggct acgatagctc gccactattg
     9121 ccctcccggc gcacgcgcag ggggttgagc accactttgc ggcggcgtag ccctttgtgg
     9181 agagcgatta gcagcaggcg cagtagctgc atttcctgtc gtcggttgct gcggagtcgg
     9241 ctgagcgatc cccgttgccg gttcaggtaa ctttggcccc atcgcgtctg gcgaaggcaa
     9301 tacctgcggc atcatctgca tcagacgttc attagcaaca cgatgcacct cttccggcgt
     9361 catcgggtta gcgccgttac gatccgcgcc ccacagcagc agcagataac gcaaacgttg
     9421 cagtcctgca gtcggacgcg cctgaatcac agtataagcg cgctgggtat cgagttttac
     9481 ggaagagaca accgcgaccg gatagccttc cgggaaacga ccgcccagac cggaggtcac
     9541 cagcacatca ccaacacgaa tatccgtatt cgctggcaga tgctcaagct gcaaatcatc
     9601 cgtacaaccg ttaccggctg caattacgcg gatatcgttg cgcagcacct ggattggcag
     9661 cgcgtgggtc gcatcacaaa tcagcagcac gcgactggtc agtttagcga cggccaccac
     9721 ctgaccaaca acacctttgt cgctgatgac cggctggcct tcataaacgc cattaacgct
     9781 acctttatcg ataacaactt gatcgctatg aggatcgtta accgtggaga taacctgagt
     9841 caccattttc tgctcatcct gacgcagcgg ggaacccagc agctcgcgca gacgcgcgtt
     9901 ctcctgtttg tattgtccaa gcatcagcag ttcactgttt ttcagcaaca gttcctgacg
     9961 taacgtccgg ttttcaagtt ctagttggtc acgcgaggcc agcgtctgcg atacgccatc
    10021 cagcaattca cgaggagcat tggaaacaaa gtagaaagga ctgacggcgg tatccatata
    10081 agtacggatt tgactgaacg tccccaggcg actgtcggca ataataatgc cgagcgccac
    10141 cagcaccgcc agaataaggc gaatctgtag cgacgggcca cggctaaaaa ttggcttcat
    10201 aagttatgcg tattctcgta tcagaccagg cagggtaaac agacatttcc cctgcctgca
    10261 tccgattact cttcgctgaa caggtcgccg ccgtgcatgt cgatcatttc cagcgctttg
    10321 ccgccaccgc gcgccacaca ggtcagcggg tcttcagcaa caacgactgg aatgccggtt
    10381 tcttccatta acaaacggtc aaggttacgc agcagtgcgc caccaccggt gagcaccatg
    10441 ccgcgctcgg agatgtcgga agccagttcc ggcgggcact gttccagtgc aaccattacc
    10501 gcgctcacaa taccggtcag cggttcctgc agtgcttcga ggatttcgtt ggagttcagg
    10561 gtaaaaccgc gtggaacacc ttctgccagg ttacggccac gaacttcgat ttcacggact
    10621 tcatcgcccg ggtaagccga accgatttcg tgcttgatac gttctgcggt ggcttcaccg
    10681 atcagagaac cgtaattacg acgcacatag ttgatgatag cttcgtcgaa acgatcacca
    10741 ccaatgcgca cggaagagga gtaaaccacg ccgttcaagg agataacagc aacttcagtg
    10801 gtaccaccac cgatatcaac caccatagaa ccggtcgctt cagaaaccgg caggccagca
    10861 ccaattgcgg cagccatcgg ttcttcaatc aggaagactt cacgggcacc agcgccctgc
    10921 gcggattcac gaattgcgcg gcgttcaacc tgggtcgcgc caaccggtac acaaaccaga
    10981 acgcgcgggc ttggacgcat aaagctgttg ctgtgcactt gtttgatgaa gtgctggagc
    11041 attttttcag tcacgaagaa gtcggcgata acgccgtctt tcattgggcg aatggcagca
    11101 atattgcccg gcgtacggcc cagcatctgc ttcgcgtcat gacctactgc agctacgctt
    11161 ttcggtgaac cggcacgatc ctgacgaatg gccaccacgg aaggctcatt caatacgatg
    11221 ccttgtcctt ttacataaat gagggtattc gcagtaccca ggtcaatgga caagtcattg
    11281 gaaaacatgc cacgaaattt tttcaacata ctaagggata atcctgaaag ctggggcgga
    11341 aaagaaaatc cgcttacttt accaaccaca cgcagcagcg acaaggcgca aaaatcatct
    11401 gctacggtga aaattagtgc agttcgtttc ctttgttaca aatctctgcc tgagtccaga
    11461 aaggcttaat gcatcagcag cattcctcgc ctgtttgcaa ccgcgtaagg tcattcatct
    11521 gcatatgtgc tgcaacaatc tggcgagcag acaagcacac tcccatgaga cgcagcgcgc
    11581 gttattctac gtgaaaacgg attaaacggc aggttaaacc gagtatcttt gtgaatattt
    11641 tttcacgtta gtatcaagtg gctgtgagga cgcgaaaaaa tccccttgcc cgcctgtaac
    11701 accgcgctga atcagggtct gccactcgct tcgcgaacgc acaccggtgg cgtaaacctg
    11761 gatgctggtc ccggagcagg cttccaccag gctttgaacc agcagctggt tctccgttcg
    11821 cttctcaatg tttctgacca gccccggatg gagcttgagt aactcaacat taagttcttt
    11881 gatccaactg gtgcttacca gcgtcaaacc agcctggttg acggctaccc gtacccctaa
    11941 agcattcact aaacgaataa caggttgtaa acgactgata tgttgaccta catcggcctc
    12001 tgcaagttca ataattatgc gttttcgttg tgatttttca cattgcatta acgtatcgcg
    12061 cagccaacgc tgaaaacgcg ggcgaatcag cgactcaacg gtaacctgaa tcgccagatt
    12121 ttcctctggc cagtaacgca atagtggaat aagacggctg atttgcagac ggtcatactc
    12181 ttccgataag ccaaactgca agaccatcgg catatactcc gccgagctaa cctcttcatt
    12241 accatcgaag atgcggcaca tgagttcgcg atgatgaacc tgaccttcgc gagtaaccgc
    12301 cggtttttga taaaggcgcg ggccgccgcg actgagcatt tgctcgataa gcgtacgcca
    12361 gcgaacatta ccgcgtcctt tttcaggcaa cgagtcatcg taaacagccc agctattgcc
    12421 gccctgcaat cccgcattac gcgtggcaga ctctgcatgt tccattacct gctcggtatc
    12481 ctgaccacta cgccaggcgc agataccaat gtggatcata tcgtcgcgat cgagcatttt
    12541 attgttcggc aaggtatcga cggctttgat taactgaccg gcgatgctct ctgcctcttt
    12601 taacgtccgg tgcggtaaca gcgcagcaaa atcactgcgg tggtaacgcg ccagcagtgc
    12661 gccagggtag cgcatcataa atgtcgacag cagattcgtc agagtgaaga actgttcttc
    12721 aacctggctg tgcccccaag tatcgctcaa catattgaaa tccggcagac gaatcatcat
    12781 cacgatcccg tgggtaccta ctttctcctg atcttccagt aacgttgcta actgattatc
    12841 gaaaaagagt cggttattga ggccggtttt cacgtcctgg gcggcataag agcggatcag
    12901 cgtatcaaga cggctgtgtt gttcgcgtgc gttctgaatt tcacgaagca gcgtatccag
    12961 cgcactgctg gttctgggcg gccattcata gatggttccc aacacattag agccacgctc
    13021 accgtttaag atacgagtag cccgggtttc cagcaattct tgcccggcaa gttgccgttg
    13081 taaccagcgt accgccaaga agagcataac aatgataaag ctaatcgccc ccgtgagcgg
    13141 cgcggtggtc atcaacgaat ggaaatagtt gcccatcgga tcctgataaa ccagacgcag
    13201 cgacatcccc ggatgcttta tcaacggaac gctcagttca cgaaacagat cgctggagcc
    13261 aaccggacga taactaccat ttctggccag ggtataaacc tgtttatcac catggagcag
    13321 gtctacacga acgatatctg ccgacatcat taattcggta atttgtggcc ttaatgtgct
    13381 gaagtcattc gacacaaggt gggtatcaat cgccgtcgcc accgcctgaa cgcgatgact
    13441 aaacttatac tgaattgcgt tgtagaaact tagcgaacag cccagcaaag tcacaaaaat
    13501 tgttaacccg gtgagcagcg taacaaaggc cgaaaatttc gtcgttaatc tcatccttgt
    13561 gttaactccg atagtgagga agcgggcata ctagcaaatc agatttatct cgcaatttat
    13621 tgcgcttcat cggctttgct tttccattag cgagtataat cttcagaaat tattttccaa
    13681 tccatcatgc acatgaggac cacttatgca ggcgttactt ttagaacagc aggacggcaa
    13741 aactctcgca tcagtacaga ctctggacga aagtcgcctg ccggagggcg atgtcacggt
    13801 cgatgttcac tggtcgagcc tgaactataa agatgcgctg gcgattaccg gtaagggaaa
    13861 aatcatccgt aattttccga tgattcctgg gatcgatttt gccggaactg tacgcaccag
    13921 cgaagatccg cgttttcatg ccggtcagga ggtgttactc actggctggg gcgttggtga
    13981 aaaccactgg ggtgggctgg cggagcaggc gcgagtgaaa ggtgactggc tggttgccat
    14041 gccgcaaggg ctggacgcgc gtaaagcaat aattatcggt actgccggtt ttaccgccat
    14101 gctgtgtgtg atggcgctgg aagatgccgg tgttcgcccg caggacgggg agattgtcgt
    14161 gacgggtgcc agtggtggcg tcggcagtac cgccgtggcg ctgctgcata agttgggtta
    14221 tcaggtcgtt gccgtttccg gtcgcgaaag tacccatgaa tatctgaaaa gtttaggtgc
    14281 cagccgtatt ctccctcgtg atgagtttgc cgaatcccgt cctctggaaa aacaagtctg
    14341 ggctggggca attgacactg ttggcgacaa agtgctggca aaagtgctgg cgcaaatgaa
    14401 ttacggcggc tgcgtggcgg cctgtggtct ggcgggtggt tttactctgc caaccacggt
    14461 catgccattt attctgcgta atgtccgttt gcaaggggtg gattcagtaa tgacgccacc
    14521 agaacgccgc gcacaagcct ggcagcgact ggtcgccgat ttaccggaat cattctatac
    14581 ccaggcggca aaagagatat ctctgtcaga ggcaccgaac tttgccgagg ccatcattaa
    14641 taaccagatc cagggtcgca cgctggtgaa ggttaactaa ccatttagca aggaataata
    14701 agagagggaa ctcattttga catcattatt caatcggtta acgggaaaag cggttagctg
    14761 gacagctttc gtcgaacacc ttggtcagga agttgtacaa catcatccaa actggaaagt
    14821 catgatttcg actgaccaca aattgatgcg cattgatact cctctaaaca gccattattg
    14881 atacgcctcc gtcgcctgtt aggtttatgg tgctttgcct gggcgacact acgcttaacc
    14941 tgttactgat ttctggtacc atggggtgaa taatctgatt ttatttgact acaaattaac
    15001 cactcgaacc tatttaatgc tgagaattgt cagtcggttg attttgcgtg ctttagcatt
    15061 cacatctatc cagacgatgc agtgaaaatt gggtaatccc cagcaaccgc tgcgtaatgt
    15121 cgtctatctt gtcgcgatcc tggcatccct acattatttg tggtctgtga agattatctc
    15181 attgcagccc ctcatcttcg cagggctggc tttacagctt ttcaccttac gttataagaa
    15241 gttccgtcga tgatggcgct aatttcgtga attgtgcggc ttgttgcaaa ttacacggtg
    15301 ttgaaggtta tttacatgtt agctgttgat tatcttccct gataagacca gtatttagct
    15361 gccaattgct acgaaatcgt tataatgtgc gacctcgtcc tccctgacgc agtttttgcg
    15421 ctgcggaaaa ggtgacattg gcgcaacgaa ggtatatttt gttttttgcc ggaggatagc
    15481 agcagatcgc tgcacaatgt ccgtcaagtc taacattgat actctggggc aaaatagacc
    15541 ggcgtcccgg cctgctggaa tttatcgcta tgcatacagc tgtcggggca tacgctttac
    15601 agacggcggt gaaacgcctg tcacaatcac actaaacaaa gagtacggaa cccactcatg
    15661 gatattcgta agattaaaaa actgatcgag ctggttgaag aatcaggcat ctccgaactg
    15721 gaaatttctg aaggcgaaga gtcagtacgc attagccgtg cagctcctgc cgcaagtttc
    15781 cctgtgatgc aacaagctta cgctgcacca atgatgcagc agccagctca atctaacgca
    15841 gccgctccgg cgaccgttcc ttccatggaa gcgccagcag cagcggaaat cagtggtcac
    15901 atcgtacgct ccccgatggt tggtactttc taccgcaccc caagcccgga cgcaaaagcg
    15961 ttcatcgaag tgggtcagaa agtcaacgtg ggcgataccc tgtgcatcgt tgaagccatg
    16021 aaaatgatga accagatcga agcggacaaa tccggtaccg tgaaagcaat tctggtcgaa
    16081 agtggacaac cggtagaatt tgacgagccg ctggtcgtca tcgagtaacg aggcgaacat
    16141 gctggataaa attgttattg ccaaccgcgg cgagattgca ttgcgtattc ttcgtgcctg
    16201 taaagaactg ggcatcaaga ctgtcgctgt gcactccagc gcggatcgcg atctaaaaca
    16261 cgtattactg gcagatgaaa cggtctgtat tggccctgct ccgtcagtaa aaagttatct
    16321 gaacatcccg gcaatcatca gcgccgctga aatcaccggc gcagtagcaa tccatccggg
    16381 ttacggcttc ctctccgaga acgccaactt tgccgagcag gttgaacgct ccggctttat
    16441 cttcattggc ccgaaagcag aaaccattcg cctgatgggc gacaaagtat ccgcaatcgc
    16501 ggcgatgaaa aaagcgggcg tcccttgcgt accgggttct gacggcccgc tgggcgacga
    16561 tatggataaa aaccgtgcca ttgctaaacg cattggttat ccggtgatta tcaaagcctc
    16621 cggcggcggc ggtggtcgcg gtatgcgcgt agtgcgcggc gacgctgagc tggcacaatc
    16681 catctccatg acccgtgcgg aagcgaaagc tgctttcagc aacgatatgg tttacatgga
    16741 gaaatacctg gaaaatcctc gccacgtcga gattcaggta ctggctgacg gtcagggcaa
    16801 cgctatctat ctggcggaac gtgactgctc catgcagcgc cgccaccaga aagtggtcga
    16861 agaagcacca gcaccgggca ttaccccgga actacgtcgc tacatcggcg aacgttgcgc
    16921 taaagcgtgt gttgatatcg gctatcgcgg tgcaggtact ttcgagttcc tgttcgaaaa
    16981 cggcgagttc tatttcatcg aaatgaacac ccgtattcag gtagaacacc cggttacaga
    17041 aatgatcacc ggcgttgacc tgatcaaaga acagctgcgt atcgctgccg gtcaaccgct
    17101 gtcgatcaag caagaagaag ttcacgttcg cggccatgcg gtggaatgtc gtatcaacgc
    17161 cgaagatccg aacaccttcc tgccaagccc aggcaaaatc acccgtttcc acgcacctgg
    17221 cggttttgga gtgcgttggg agtctcatat ctacgcgggc tacaccgtac cgccgtacta
    17281 tgactcaatg atcggtaagc tgatttgcta cggtgaaaac cgtgacgtgg cgattgctcg
    17341 catgaagaat gcgctgcagg agctgatcat cgacggtatc aaaaccaacg ttgatctgca
    17401 gatccgcatc atgaatgacg agaacttcca gcatggtggc actaacatcc actatctgga
    17461 gaaaaaactc ggtcttcagg aaaaataatt tgtgacgcgc ggtgttttca tcgcgcttag
    17521 caaagaacgt aatcaacgcc gggcctgtta acgctgcccg gcgtttttat tgccgttagc
    17581 taacggtagc ctgcaatttc cgctttcaac gcgctcattt cagcatcgcc atcaacaact
    17641 cccagctcaa agcagtattt ctttgtttcc cctggctcaa taaagggcag acgcccctgc
    17701 gctcgttctt tatcacgacc gtcaggaaaa ctattactga cctccagtcc cataacatac
    17761 tcacccgcgc ctaaattttt ccactggata aagttgggta gctgcatggt gtcgaatttc
    17821 tcgtaaattc cccaccccag ttcggcgttc accagagcaa ctttactcat cccctgactg
    17881 tcgctattca acgtgagata gtaaacctgc tcgtcaaaac cgggctgtgg ggtacaaatc
    17941 tccagatgac tggttaaccc ttccgccgca tgcggagttc gtggcgttat gcttttagcc
    18001 gacagcaaga tttctggcct gtggcgataa taaaggccag ccgtaattaa agtgataaag
    18061 aatttgcagc ggctggcggg tgaaaccatg attagtcacc ttgtcttcaa tgcgcagcac
    18121 attttcgcca taacggcagc gaatggtcct ttccagcgtc agattttcgc cgaacagtcg
    18181 cgtttcccgt actttgccat tgatgacaaa ttcaatgccg tcgtcggtcc gttctgtgcg
    18241 ataccccacc tcttctgctg gagtcgcaga caaacgcccg tgtaacccca gcgtttctcc
    18301 ttcatcctca caaggcgtgc caatgtagct aagtccgcag gtagtcagta accccgcgta
    18361 aaagctacgt aaaaatccgt tgccctgaga ctcaaaaaaa gcgggggcga caatgccgtt
    18421 gggggtgata aaagaaagcg ccgtgtcctt gtagctggcc catgcaatat ccagcgcgcg
    18481 atcttttacc accgtaaatg ccagtcccgc accagtacgg acatcaaacg cttctgtacc
    18541 ctttgctctg ccactacgta actcatagtg tttaattccg gcaacctgcg agagatcgcc
    18601 aatccacgac caaatttcgt ttttcattgt gctgcttcct gaatggacat gtcatggcgc
    18661 tggatgaacg cttcgatttc cgcaaggcga gacgttccct ccattgggcc acgtctggtt
    18721 accgccagcg caccgcaggc attggcatat tgcagcgctc gatggacgtc aaaccctagc
    18781 tggcggcagg caatccacgc gccaccaaag cagtctcccg caccggtcgg gtcaacttct
    18841 tccaccggat agctctcaac atgaaactgc tcattcgcgg agtagtaact ggcaccttga
    18901 ttgccgcgct tcacaatcac ctctttaaca ccttcttcaa gaaaacctgc gattgcacgc
    18961 tccggcgtcg aatgtggcga caagagcaac acttcgccct cgctgggcat atagatatcc
    19021 gttaactcaa gaacaaagtg gagggcgtca cgcatttctg gaatatcgag catctcttta
    19081 cgaatattag ggtcgaaaga
//
LOCUS       DAJTNA010000070        18911 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTNA010000070 DAJTNA010000000
VERSION     DAJTNA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18911
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.071"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO161_002058"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO161_002058"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5463125.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO161_002059"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO161_002059"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5463126.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO161_002060"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO161_002060"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5463127.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO161_002061"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO161_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5463128.1"
                     /translation="MTQLTIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLIWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO161_002062"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO161_002062"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5463129.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNNGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO161_002063"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO161_002063"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5463130.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="OO161_002064"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="OO161_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5463131.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO161_002065"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO161_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5463132.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO161_002066"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO161_002066"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5463133.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHS
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO161_002067"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO161_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5463134.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO161_002068"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO161_002068"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5463135.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="OO161_002069"
     CDS             complement(15666..16703)
                     /locus_tag="OO161_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5463136.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLKILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO161_002070"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO161_002070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5463137.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNCGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17760..>18911
                     /locus_tag="OO161_002071"
     CDS             17760..>18911
                     /locus_tag="OO161_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HCR5463138.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPM"
BASE COUNT         4826 a         5009 c         4599 g         4477 t
ORIGIN      
        1 accctttggc cccgttctat ttattggtga acttacaatc tcaccggatc gatatgccag
       61 atatgatcgg cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agaacctgat agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcta gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg gcggcttaaa atgtcctgaa tggtcgagga aaccagaaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accttggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcaa cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgagaccac agtacaaaag cacgtcgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcaccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct ggccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagacccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc gcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgccggaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaagttg tgcacagtaa acaccgactt cgccggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaagg cctgcatgcc agtcgtgcgc atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgtcc ggcgaaggta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacttgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagctttcag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccggagt
     5041 tgatgcagtt agacagcgca aagtcgataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgtg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca gccgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccatcg ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gattaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtaccctg
     5701 ggagagcaac aacgttgtta acaaggcgtg aatactgtcg cgccgccgtt caaccaggtc
     5761 aagagaaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcag tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccaccagg ggcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtgacaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagtgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccggaatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcatcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgtgccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggtgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagt
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgc atggaatcgg tattgaggat ttcacgccat
     7381 ttgcctggct gattaatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccac cttccaacag atgccagtcg aggctggcgt catggttcca ctcacggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg ggaatgccca catccagcca
     7741 tagtaggcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagaa tcccgaaggt gagtttatcg tgatgatact gacgataaac cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcgcgataaa tcattgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc attattgtag
     8281 atcagcgtat tccagtcctg atgatagcct tcgcgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcggtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagacccgc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcacggtag ctcaaccaga aattgttatc ggtgtggcga
     8641 cgccaggagc ccaggtgaac ttcataaata gagattgacg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccaa
     8881 atgccgctct ctttacgcag acgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccaaacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcgggccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacac cgctaaagaa tccccgtgag tcaagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg ttgttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcacccacg gtaaaggctg acaggaactc cggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgacca ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagcagcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttatca cacagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaacc
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagaacccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc ggcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccggagtagt tcagttgttc aatgaccggc atcaccagcc ccaccgccgt taaacaggca
    11881 acggtggcag acccctgaat gatgcgcact gcagcggcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgt
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa atggatgacc aataaactcg
    12181 aaccattcgt aagcagttga tccttctggc acaaaacgag cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaggacgg cattttgcct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca actccacgta acggctgatg
    12361 aaattacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca cagaccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcatcgg cgctggtcca
    12481 ggcaccagaa atgccgccgc tgccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aactatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc aaccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tgtaagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcaa gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa cagacgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggcgtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg aatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaatcaaaa atgttacggg taactgttat cggtaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtagactt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgctaaccgc ggctccatca cctggccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga accttcaatc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccaggtc atcgttggta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatacg acaccagacc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg cgcgccgaga taggcaatgt ggcaatgccc gcgagcaata
    14101 atggcggtgg tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat gagttccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcagtc cgtcgatatt ccatgagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgagc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tggcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtgac attgcctttc accacctgga tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc agcggtacag
    15061 ttccatatcc tgatgcactt tcaatgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgt acggcatcga agcgacgctg ttcataacgc ggcgtaatac cgttttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctgcttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcaaaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg ccccctctgt tcgaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 attttcagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacgcgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca cgccctcgtc attgacatac tccggcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccataaaa cgcgccatcc
    16201 tgcggcagcc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcaacaatct ctcccaactt gccactttca atcgcttttt tcgctgtcag gaagcaggag
    16321 tcaaagcgac gattctgata cggcgtgacg gtcagccctt tgcttttcgc caacgcaaac
    16381 agctccttcg cctgggcaag cgtcggggtg aacggttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgtttcgc gtactcgaaa tggctgtccg cgtgagtgca gacaacaacc
    16501 agcttaacat cgggatcgtt tagtacttcg tcgagatcgc tggtgaaatg gatatgggaa
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac tgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttgcgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgccc cggcaattaa ggtctataaa aaatacggct ttgaaattga agggactggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcat attatatggc gcgggtgaag
    17521 taagatagtg ccctttttct gagatggaaa aagggtgtca ttcaaaatcg acataccttc
    17581 ctttaaggta tttatttatc caatacatat attgagattt aactcataat tacttcctaa
    17641 agtgtaatat tttatttttt aatatatacg cctacaattt cctggagtaa ataaataaca
    17701 attaacaagc ataatattgc cattgataaa atagcatgcc ataaaaggac ttttcaggga
    17761 tgagtaatat tgtttacctg acagtaacgg gagaacaaca aggaagcatc tccgcaggtt
    17821 gtgggacttc tgagtctaca ggtaatcgtt ggcagagcgg gcatgaggat gaaatattta
    17881 cattctcact cttaaataat attaataata cggggcttgg ttcacagttc catggtataa
    17941 cattttgtaa attaattgat aaaagcactc cattatttat taattccatt aacaataatg
    18001 aacaattatt tatgggattt gacttctatc gaataaatag atttggtaga ttggaaaagt
    18061 attattatat acaactaaga ggcgcttttt tatcggctat tcatcaccag atcattgaaa
    18121 accaactgga tacagaaaca ataactatta gttatgaatt tatcctctgt caacatctta
    18181 tcgcaaatac cgagttcagc tatttggcac tccctgaaaa ttataaccgt ttgtttttac
    18241 caaattcaaa aaaccaaaca aataatcgtt tcaaaacgtt aaacagcaaa gctattggca
    18301 ggctacttgc tgctggtggc gtatacaatg ggaacattga aggattcaga gatactgcgg
    18361 aaaaactggg tggagatgca ataaaaggct atgatcaaat actaaatgaa aaaacagcgg
    18421 gcatagcgat agcaacagca tctattcttt taacaaagcg ttctaatgtt gatacatata
    18481 cagaaataaa tagttactta ggcaaactta gaggtcaaca aaaacttctt gatggtatag
    18541 acataataga aataatatac attaagagac cttcaaaaga cttagctaac ttacgaaagg
    18601 agtttaataa aactgtaaga aaaaattttc ttatcaaact tgcaaaaacc tccgaagcat
    18661 ctggaagatt caacgccgaa gaccttttaa gaatgagaaa gggcaatgtt cctctaaatt
    18721 ataatgttca ccataaacta tctctagatg atggtggtac taatgatttc gaaaatttag
    18781 tattaatcga aaacgaacca tatcataaag tttttactaa catgcaatca cgaatagcta
    18841 agggaatatt agtaggtgaa agcaaaatca ctccctgggc cattccatct ggctcaattt
    18901 atcctcccat g
//
LOCUS       DAJTNA010000071        18744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTNA010000071 DAJTNA010000000
VERSION     DAJTNA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18744
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.072"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..733
                     /gene="rihB"
                     /locus_tag="OO161_002072"
     CDS             <1..733
                     /gene="rihB"
                     /locus_tag="OO161_002072"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5463139.1"
                     /translation="IMRKQIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMA
                     SDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPE
                     AARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFEN
                     YGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTK
                     VGITIDTDWFWGLVEECVRGYIKTH"
     gene            833..2083
                     /locus_tag="OO161_002073"
     CDS             833..2083
                     /locus_tag="OO161_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5463140.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPAGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDHLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGGDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2139..3227)
                     /locus_tag="OO161_002074"
     CDS             complement(2139..3227)
                     /locus_tag="OO161_002074"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5463141.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3230..4087)
                     /gene="nfo"
                     /locus_tag="OO161_002075"
     CDS             complement(3230..4087)
                     /gene="nfo"
                     /locus_tag="OO161_002075"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5463142.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4161..5210)
                     /locus_tag="OO161_002076"
     CDS             complement(4161..5210)
                     /locus_tag="OO161_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5463143.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5310..6191
                     /locus_tag="OO161_002077"
     CDS             5310..6191
                     /locus_tag="OO161_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5463144.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            6397..7866
                     /gene="lysP"
                     /locus_tag="OO161_002078"
     CDS             6397..7866
                     /gene="lysP"
                     /locus_tag="OO161_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5463145.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8159..10139
                     /gene="cirA"
                     /locus_tag="OO161_002079"
                     /pseudo
     CDS             8159..10139
                     /gene="cirA"
                     /locus_tag="OO161_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10171..11007)
                     /gene="yeiG"
                     /locus_tag="OO161_002080"
     CDS             complement(10171..11007)
                     /gene="yeiG"
                     /locus_tag="OO161_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR5463146.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYYLGQGAGF
                     YLNATQPPWATHYRMYDYLRNELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11265..11933
                     /gene="folE"
                     /locus_tag="OO161_002081"
     CDS             11265..11933
                     /gene="folE"
                     /locus_tag="OO161_002081"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5463147.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11950..13107
                     /locus_tag="OO161_002082"
     CDS             11950..13107
                     /locus_tag="OO161_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5463148.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13249..14289
                     /gene="galS"
                     /locus_tag="OO161_002083"
     CDS             13249..14289
                     /gene="galS"
                     /locus_tag="OO161_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5463149.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSTHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14569..15567
                     /gene="mglB"
                     /locus_tag="OO161_002084"
     CDS             14569..15567
                     /gene="mglB"
                     /locus_tag="OO161_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5463150.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15628..17148
                     /gene="mglA"
                     /locus_tag="OO161_002085"
     CDS             15628..17148
                     /gene="mglA"
                     /locus_tag="OO161_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5463151.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLMGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17164..18174
                     /gene="mglC"
                     /locus_tag="OO161_002086"
     CDS             17164..18174
                     /gene="mglC"
                     /locus_tag="OO161_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5463152.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18417..>18744)
                     /locus_tag="OO161_002087"
     CDS             complement(18417..>18744)
                     /locus_tag="OO161_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5463153.1"
                     /translation="LADQGFDSLQEMVGLANNNIVPAEDLDRSYIVYPRINLDKCVGC
                     GRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGCIDLGEVKFKKGEK
                     EHPVTL"
BASE COUNT         4477 a         4606 c         4979 g         4682 t
ORIGIN      
        1 cattatgcgt aaacaaatcg ttgccgataa tattcacggt gaaaccggac tggatggtcc
       61 ggtattcgag tcgctgaccc gccaggcaga aagtacccac gcggtgaaat acatcattga
      121 taccctgatg gcaagcgatg gcgatattac tctggtgccg gttggtccgc tttcaaatat
      181 cgcggtggca atgcgtatgc aacctgcgat cctgcccaaa atccgtgaaa ttgtgctaat
      241 gggcggcgct tacggtacag gcaacttcac gccatctgcc gagttcaaca tctttgctga
      301 tccggaagca gcccgcgtgg tgttcacctc cggcgttcca ttagtgatga tgggcctcga
      361 tctcaccaac cagactgttt gcaccccgga cgtgattgca cggatggaaa gggcaggcgg
      421 ccctgccgga gagctgttca gcgacatcat gaacttcact ctcaaaacgc agttcgaaaa
      481 ctacggcctg gctggcggcc cggtgcacga cgccacctgc atcggttatc tgattaaccc
      541 tgatggcatt aaaacccagg agatgtacgt cgaagtggac gtcaacagtg gcccttgcta
      601 tgggcgtacc gtctgcgacg agctgggcgt tcttggcaag cccgctaata ccaaagtcgg
      661 catcactatt gataccgact ggttctgggg cttagtcgaa gagtgcgtgc gcggctacat
      721 caaaacccat taagtgttga taacaagccg ggtcagtatc gcaagatcca agcccggcta
      781 tttctgatgg gcagaaagcc gtcgcccaat atgacatgaa gagataataa ctatggatgt
      841 catgagaagt gttctgggaa tggtggtatt gctgacgatt gcgtttttgc tgtcagtaaa
      901 caagaagaag atcagcctgc gtaccgttgg cgcggcgtta gtgttacagg tcgtgattgg
      961 cgggattatg ctttggttac cggcagggcg ttgggtcgct gaaaaagtcg cttttggcgt
     1021 gcataaagtg atggcgtaca gcgatgcggg tagcgcattt atcttcggtt cgctggtcgg
     1081 gccgaaaatg gacacgctgt ttgatggcgc aggatttatc tttggtttca gggtattacc
     1141 ggcaattatc ttcgtcactg cactggtgag tattctctac tacatcggtg tgatggggat
     1201 tttaattcgc attctcggcg gtatattcca gaaagcatta aatatcagca agattgagtc
     1261 attcgtcgcg gtcaccacca ttttcctcgg gcaaaacgaa ataccggcga tcgtcaaacc
     1321 ctttatcgat catctgaatc gcaatgaatt atttacagcg atttgtagtg gcatggcctc
     1381 gattgctggt tcgacaatga ttggttacgc cgccctgggc gtacctgtgg aatatttgct
     1441 ggcggcatcg ttaatggcga tcccaggcgg gatcttgttt gcccgcctgt taagcccggc
     1501 tacggaatct tcgcaggttt cctttaataa cctctctttc accgaaacac cgccaaaaag
     1561 cattattgaa gctgctgcga caggggcaat gaccgggctg aaaatcgccg ccggtgtagc
     1621 gacagttgtt atggcatttg tcgccatcat tgcgttaatt aatggtatta tcggcggcgt
     1681 tggcggctgg tttggttttg cacatgcctc gctggagtcc attttaggtt acctgttggc
     1741 cccattggcg tgggtgatgg ggggtgactg gagtgatgca aatcttgccg ggagtttgat
     1801 tgggcagaag ctggcgatca atgaatttgt cgcttatctc aatttctcgc catatctgca
     1861 aacgggtggc actctggatg ctaaaaccgt ggcgattatt tctttcgcgt tgtgcggttt
     1921 cgctaacttt ggttctatcg gggtggtggt gggggcgttt tctgcggttg cgccacaccg
     1981 tgcgccggaa atcgcccaac ttggtttacg cgcgctggcg gcggcgacac tttctaacct
     2041 gatgagtgct actattgcag gattctttat tggtttagcg tagtgcgcaa tttatgagcc
     2101 ggataagatg cggtcaagcg tcgaatccgg caacattgtt acaccattgg cactaatgaa
     2161 agcgcgttat cggcagacag ggtagggttg tttaagctgc cgctggcgcg cgaaattgcc
     2221 gcgcaggcta ccgcaaaacg ggcgctgtcg cggaaactgt atccttcaag aaagctatat
     2281 accaggcccg ccataaaacc atcgtcagca ccaaaactgt ctactgtcgt atgcgctggc
     2341 gcggtcagca aaaattgttc tccatccttt tcgctgcaat aaactgactc atcgggcaaa
     2401 taaacaaaca gttgctgaac accttgctga tgcaatgcat tcactgcggc attacggtca
     2461 gcatcgctgg tgatcgcctg tccccataaa atttccagct ccggtaaagt gggtttcagg
     2521 gtgtgaatat gcgccagcca gtgtttgatt ttgcccgctt tgaattctga aacggtatcg
     2581 acaaacaccg ggatttcatc agcgagggta aagacccatt ccagcgcctc ggctgtcagg
     2641 ttgcaatcag ccagtaccac gcccgcatga cgaagtaaat cgcgcgaccc gttcagtagt
     2701 tgcggtgtca actgttccag cagatgggta tcgttaatcg ccagcacggt ttgatcgtct
     2761 cgattggcaa ttgccagata cgtcgatgtg ctttgaccat gcaaacgaac gcagccggag
     2821 acattcacgc cggcacggcg cgtttcttcc aggagcattt cgccataaaa gtcatcgcca
     2881 atcactgaaa gcaaatggac gtcacggcct aacagcgcca gattgtgggc gatgttgcgt
     2941 cccacgccgc ccgccgagca atgaattgta ccgggatgag aagccgcttg cgggtaacgg
     3001 atatccgcca tcccgcgaat atccatattg attgtcccca ccactacgca gtattcctgc
     3061 tcggtgagaa tgtaaccttt gcctttaatc cgccctttgc gcattaaatc cataatatgc
     3121 gccgcaacgc gcgaacgact gatttgcagc atgtccgcaa tttcgttctg ctgaatcagc
     3181 gggttacgcc gtaaaattgc aaggatctcc ttttcccggt tattcatctt caggctaccg
     3241 ctttttcagt ttgttgcgct ttcagccagg cgatctcttc tgcccagata tccgggttga
     3301 tggtttcaag gatcagcgga atgccgtcga aacggtcgtc ctgcatgatc cagcggaacg
     3361 catcatgacc gatattgcct tcaccgaggc tatgatggcg gtcaacgcgg ctgccaaagg
     3421 tgcttttcgc atcgttaagg tgcatcccgc gcagatactt aaagccaaca atacgggcaa
     3481 aatccgcgaa cgttttctcg cattcggctg gagtacgcaa atcatacccg gcagcgaaag
     3541 catggcaggt atcaatgcag acgccgacac gggatttatc ttccacgccg tcgataatcg
     3601 ccgcgagatg ttcgaattta aaccctaagt tactgccctg accggcggtg ttttcaatca
     3661 ccgctgttac accttgagtt ttatccagcg caatgttgat ggattcggca atacgcgcaa
     3721 ggcaatcctc ttctgaaatc tgcatcagat ggctgccagg gtggaagttg agcaaagaaa
     3781 gccccagttg ttcgcaacgc tgcatttcat ctataaaggc atcgcgcgat ttttccagag
     3841 cttcagtgac cggatgtccg aggttaatca gatagctgtc gtggggaaga atttgcgccg
     3901 atgtgtagtg atatttttca caggccgctt tgaattcatc gatggtttgc gtcgtgagcg
     3961 gtgcggcacg ccactgacgt tggtttttgg tgaacaaggc aaacgcggtt gcgtcgattt
     4021 cggcggcgcg aattgcggca tttgccagac cgccagcagc actaacgtgc gctccaatgt
     4081 atttcatgcg aggactcctg ttaaacccgc tggaggaaaa cggtaatgat agcgggttaa
     4141 caggagcaag atgtagtggt ttatgcgatg acgctttgaa tcacatagtt aatcgcacca
     4201 ccaccaacaa tcagccaggc aaacagtacc agtgccatca gcagaggttt cgccccggct
     4261 tttttcagcg cgctgacgtg agtggtcaga cccagcgccg ccatcgccat tgccagcagg
     4321 aaggtatcca gcgttaccag catgttcacc acgctctgcg gtaacaggtg gaacgagtta
     4381 aagatggcaa cgacgatgaa caagatggca aaccacggaa tagtgatttt gcttttctcg
     4441 ccgctgttcg ccccagacag ctgtttaaca cgcgccgcca gcaggatgag gaacggagcc
     4501 agcatcatca cgcgcagcat tttggaaata actgctgcgt tttccgcatc cgggctgatg
     4561 gcatgacctg ccgccaccac ctgcgccact tcgtgcacag tagaaccaat gtagataccg
     4621 aaagtttccg gactaaacca ttgagacatc agcggatata tcgccgggta gaggaaaatc
     4681 gcgacggtcc cgaagataac aacggttgca acagccacgg ttactttact ggcttccgct
     4741 ttcactaccg gctcagtcgc cagtaccgcg gcagcaccac agatactgct accggcaccg
     4801 atcaaccagc tggtgtgctt atccagacca aacactttct gccccaggaa gcaagccagc
     4861 aggaaggtac tggacagcgt caacacgtca atgatgatcc cactgatacc gacatcggca
     4921 atttgcgaga acgtcagacg gaagccataa agaatgatac ccagacgtaa taaatattgc
     4981 ttggcaaaca gcacaccacc gtcacagctt ttccagatgt gcggatagat ggtgttgcct
     5041 aaaaccatcc ccaacaagat tgcgagggtg agggcactaa acccggcacc cgcaaccgcg
     5101 ggaatggaac caccccacag ggcgaccccg gtgataactg cactcagggc taaccccgga
     5161 ataaaatgcc acagtgtacg atgttgtttc tgtaaggtga tattcgtcat aaccctctcc
     5221 ttataaccgg gcataaggtt acgactaact ggtttaaaaa taaaattgat tatatattta
     5281 taattaatct ttataagtgg taagcgacta tgcacatcac cctccggcag ttggaagttt
     5341 ttgcagaagt attgaaaagt ggatcaacca cccaggcgtc ggtgatgctg gcgttgtcgc
     5401 aatcagcagt gagcgcagcc ttgaccgacc tggaagggca gcttggcgtg caactgtttg
     5461 atcgcgtggg gaaaagactg gttgttaatg aacacgggcg gctgctctat ccgcgtgcgt
     5521 tggcattgct tgaacaggcg gttgaaatcg aacaactgtt tcgcgaagac aacggcgcga
     5581 ttcgtatcta tgccagtagt accatcggta attacattct gcctgcagtt atcgcccgtt
     5641 atcgccatga ttatccgcag ttgccgattg aacttagcgt tgggaacagc caggacgtga
     5701 tgcaagcggt gctggatttc cgcgttgata ttggctttat tgaaggaccg tgccacagca
     5761 ctgaaatcat ttctgaaccg tggctggaag acgagctggt ggttttcgcc gcgccgactt
     5821 cgccgttggc tcgtggtccg gtcaccttag aacagctggc cgctgcgccg tggatcctgc
     5881 gtgaacgcgg ttccggcacg cgggagattg tcgattatct attgttgtca catttaccga
     5941 agtttgagat ggcgatggaa ttaggtaact ccgaggcaat caaacatgcg gtgcgtcatg
     6001 ggttgggaat tagttgcctg tcgcgacggg tgattgaaga tcaattgcag gcaggcacat
     6061 taagtgaagt tgcggtccct ctgccgcgcc tgatgcgtac gttgtggcgt atacatcatc
     6121 ggcaaaaaca cctttccaac gcgctacggc gctttctgga ctattgcgat cccgcgaatg
     6181 taccgcgtta agttgctgta caagaacatg ctggtgctgt gtcgatttcg tgacgcagcg
     6241 ccctcagcat gcattcgcca gaaaagagat tggctgcttt acttataatc cctgggcgat
     6301 catgaaggtg tcttataacc gtgtatttct gccggaagga ttgccaatcg tctgctacaa
     6361 tcgcgccatc atttttaaga tggatagcat ttttgtatgg tttccgaaac taaaaccaca
     6421 gaagcgccgg gcttacgccg tgaattaaag gcgcgtcacc tgacgatgat tgccattggc
     6481 ggttccatcg gtacaggtct ttttgttgcc tctggcgcaa cgatttctca ggcaggtccg
     6541 ggcggggcat tgctctcgta tatgctgatt ggcctgatgg tttacttcct gatgaccagt
     6601 ctcggtgaac tggctgcata tatgccggtt tccggttcgt ttgccactta cggtcagaac
     6661 tatgttgaag aaggctttgg cttcgcgctg ggctggaact actggtacaa ctgggcggtg
     6721 actatcgccg ttgacctggt tgcagctcag ctggtcatga gctggtggtt cccggataca
     6781 ccgggctgga tctggagtgc gttgttcctc ggcgttatct tcctgctgaa ctacatctca
     6841 gttcgtggct ttggtgaagc ggaatactgg ttctcactga tcaaagtcac gacagttatt
     6901 gtctttatca tcgttggcgt gctgatgatt atcggtatct tcaaaggcgc gcagcctgcg
     6961 ggctggagca actggacaat cggcgaagcg ccgtttgctg gtggttttgc ggcgatgatc
     7021 ggcgtagcta tgattgtcgg cttctctttc cagggaaccg agctgatcgg tattgctgca
     7081 ggcgagtccg aagatccggc gaaaaacatt ccacgcgcgg tacgtcaggt gttctggcga
     7141 atcctgttgt tctatgtgtt cgcgatcctg attatcagcc tgattattcc gtacaccgat
     7201 ccgagcctgc tgcgtaacga tgttaaagac atcagcgtta gtccgttcac cctggtgttc
     7261 cagcacgcgg gtctgctctc tgcggcggcg gtgatgaacg cagttattct gacggcggtg
     7321 ctgtcagccg gtaactccgg tatgtatgcg tctactcgta tgctgtacac cctagcgtgt
     7381 gacggtaaag cgccgcgcat tttcgctaaa ctgtcgcgtg gtggcgtgcc gcgtaatgcg
     7441 ctgtatgcga cgacggtgat tgccggtctg tgcttcctga cctccatgtt tggcaaccag
     7501 acggtatacc tgtggctgct gaacacctcc gggatgacgg gttttatcgc ctggctgggg
     7561 attgccatta gccactatcg cttccgtcgc ggttacgtat tgcagggaca cgacattaac
     7621 gatctgccgt accgttcagg tttcttccca ctggggccga tcttcgcatt cattctgtgt
     7681 ctgattatca ctttgggcca gaactacgaa gcgttcctga aagataccat tgactggggc
     7741 ggcgtagcgg caacgtatat tggtatcccg ctgttcctga ttatttggtt cggctacaag
     7801 ctgattaaag gaactcactt cgtacgctac agcgaaatga agttcccgca gaacgataag
     7861 aaataagttt cctcccttcc ttgctaagcc ctctcaaccg agagggcttt ttcaattcca
     7921 tttccctgac aaatcatgcg gatataaaat ttaacattta gattgataat tgttatcgtt
     7981 tgcattatcg ttacgccgca atcaaaaaag gctgacaaat cagaggctgt tccggctttc
     8041 tgggatgatc acctgcatac aaataagtcc accgcgatgc tgccgtacgc aaggggacgt
     8101 gaagaagatg tgagcgataa cccattttat tttcgtagtt acctcatgga gatatggaat
     8161 gtttaggttg aaccctttcg tacgggtcgg gctgtgtttg tccgctattt cttgtgcatg
     8221 gcctgtgtta gcggtcgatg atgatggcga aacgatggtt gtcactgcat cttccgttga
     8281 acaaaacctc aaagatgcac ccgccagtat cagcgtcatt acccaggaag acctgcagcg
     8341 aaaaccggta cagaatctga aggatgtcct caaagaagtg cttggcgtac aactgacgaa
     8401 cgaaggggat aaccgtaagg gcgttagtat tcgtggtctg gacagcagct acaccctgat
     8461 tctcgtcgac ggtaaacgcg ttaactcccg caatgccgtc ttccgccaca atgatttcga
     8521 tctgaactgg atcccggtcg attccatcga acgtattgaa gtggttcgtg gcccgatgtc
     8581 gtcgctatac ggctccgatg cgctcggcgg tgtagtgaat atcatcacca aaaaaatcgg
     8641 tcagaaatgg tcgggtaccg ttaccgtcga taccaccatt caggaacatc gcgatcgcgg
     8701 tgatacctat aacggtcagt tctttaccag tggaccatta attgatggtg tgctgggaat
     8761 gaaagcttac ggcagcctgg caaaacgtga aaaggatgac ccgcaaaact caacgaccac
     8821 cgataccgga gaaacgccgc gtattgaagg attctccagc cgcgacggca atgtcgaatt
     8881 tgcctggaca ccgaatcaaa atcacgattt tactgccgga tacggtttcg accgtcagga
     8941 actcgctgga caaaaaccgc ctggaacgcc agaactactc cgtcagccat aatgggcgtt
     9001 gggattacgg caccagcgaa ctgaaatact acggtgagaa agtcgagaac aaaaaccctg
     9061 gcaacagcag ccggataact tccgaaagca atacggtcga cggcaaatac acgttgccgc
     9121 tgacggcgat taatcagttt ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg
     9181 atgcggtgaa cctgaccggg ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt
     9241 ttgtggaaga tgaatggcgg atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg
     9301 acgatcacga aacctacggt gaacactgga gtccgcgtgc ctacctggtt tataacgcca
     9361 ccgacaccgt aacggtgaaa gggggctggg cgacggcatt taaagcacct tctctgttgc
     9421 aacttagccc tgactggacg agcaattcct gccgtggcgc atgtaagatt gtgggtagcc
     9481 cggatctgaa accagaaacc agcgaaagtt gggagctggg gctttactac atgggtgaag
     9541 aaggctggct ggaaggggtt gaatccagcg ttaccgtttt ccgtaacgat gtgaaagatc
     9601 gtatcagcat cagccgtacg tctgacgtca acgctgcacc gggctaccaa aaccttgttg
     9661 gttttgagac gggcgctaac ggacggcgca taccggtatt tagctactac aacgttaaca
     9721 aagctcgtat tcagggcgtg gaaaccgaac tgaaaattcc gttcaacgat gaatggaaac
     9781 tgtcgatcaa ctacacctac aacgatggtc gtgatgtcag caacggcgaa aacaaaccgc
     9841 tatccgatct gccgttccat actgctaacg gtacgctgga ctggaaaccg ctggcgctgg
     9901 aagactggtc attctatgtt tctggtcact atatcgggca gaaacgcgcc gacagcgcga
     9961 cggctaaaac accgggcggt tataccatct ggaataccgg cgcggcctgg caggtgacta
    10021 aagacgtcaa actgcgcgca ggcgtgctga accctggcga caaggatctc agtcgtgacg
    10081 actacagcta taacgaagac ggacgtcgtt actttatggc agtggattat cgcttctgat
    10141 gagaagatgc ccggcgaacc gggcggactt tcacttcagt aaatactgcg catggaagcg
    10201 caggtgatcc tctataaaag aggcgatgaa gtagtaactg tgatcatatc ccggctgaat
    10261 acgcagcgtc atcggccacg ctttctggcg cgcggcttct gccagtaccg caggttgcaa
    10321 ctggtcggca agaaactgat cattatcgcc ctgatcgata agcgtcggga tcgcatcctg
    10381 cgcgttactg gcatacatca gcgcgcaact gtcccattcc agccatgcat ttttgtcctc
    10441 acctaaatag ctgctaaacg ctttgattcc ccacgggacg ctgcacggat tcacaattgg
    10501 cgcaaaggcc gaaacgctgg tgtatttacc cggatttttc agcgccataa tcagcgcacc
    10561 gtgaccaccc attgagtgac cgctaatggc gcagcggtcg ctgacattaa attgcgactg
    10621 aaccagcgcc ggtaattcat tgcgcagata atcatacatc cggtaatgcg tcgcccacgg
    10681 cggttgcgtg gcattaagat aaaagcctgc gccctggcct aaatagtagc catcgtcgtt
    10741 ggcaaccttt tcgccgcgcg ggctggtgtc tggcatcacc agtacaatcc ccagttctgc
    10801 cgccacgcgc tgggcacccg ccttggtggt gaagttctcg tcattgcagg ttaatccgga
    10861 aagccagtac agcactggtg gcggggtgtg atcacgaggg ggtgggagaa agatactgaa
    10921 cgtcatcggg cagtttaagg tactggagtc gtgtcgccag cgttgctgcc agccttcaaa
    10981 acagcggtgc tcttcgagca tttccatgcg tggctccttg ttgtgttgtt tgcaatttct
    11041 tcatcatacc gtttattcaa tgtgctgtga gtaactttca cttccgtatt tgcataacga
    11101 tgttttaaca tctgctgatg aaaggcagcg gcaattacaa taattatcgc tgtgaatact
    11161 ggattatgtg cgccgcctca cgcacaataa tcaggctgta aatcagctta ataactttgc
    11221 ccccacgcag ggcggaggcg tcacacctgc aggagaaatc ataaatgcca tcactcagta
    11281 aagaagcggc cctggttcat gaagcgttag ttgcgcgagg actggaaaca ccgctgcgcc
    11341 cgcccgtgca tgaaatggat aacgaaacgc gcaaaagcct tattgctggt catatgaccg
    11401 aaatcatgca gctgctgaat ctcgacctgg ctgatgacag tttgatggaa acgccgcatc
    11461 gcatcgctaa aatgtatgtc gatgaaattt tctccggtct ggattacgcc aacttcccga
    11521 aaatcaccct cattgaaaac aaaatgaagg tcgatgaaat ggtcaccgtg cgcgatatca
    11581 ctctgaccag tacctgtgaa caccattttg ttaccatcga tggcaaagcg acggtggctt
    11641 atatcccgaa agattcggtg atcggtctgt caaaaattaa ccgtatcgtg cagttctttg
    11701 cccagcgtcc gcaggtgcag gaacgtctga cgcagcaaat tcttattgcg ctacaaacgc
    11761 tgctgggcac caataacgtg gctgtctcca ttgatgcggt gcattactgc gtgaaagcgc
    11821 gtggcatccg cgatgcaacc agtgccacaa caacgacctc tcttggtgga ttgttcaaat
    11881 ccagtcagaa tacgcgccac gagtttctgc gcgctgtgcg tcatcacaac tgattaaaag
    11941 gcaggaacca tggagcgcaa cgtaacgctc gattttgttc gcggcgtcgc cattctgggg
    12001 atcctgctat taaacatcag cgcctttggg ctaccaaagg cggcttatct caatcccgcc
    12061 tggtacggcg ctattacgcc gcaggatgca tggacctggg catttctcga tctcatcggc
    12121 caggtgaaat tcctcacgct ttttgcgctg ctgtttggtg cgggcctgca aatgttgctg
    12181 ccccgtggca gacgctggat ccagtcgcgg ttaacgctgt tagtcttgct gggttttatt
    12241 cacggtttat tgttctggga cggcgatatt ctgctggctt acgggctggt gggcttaatc
    12301 tgctggcggc tggtgcgcga tgcgccatcg gtaaaaagcc tgtttaatac aggcgtcatg
    12361 ctttatctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    12421 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    12481 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    12541 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    12601 agcggctggt tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    12661 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    12721 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    12781 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    12841 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    12901 atttgtacca tgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    12961 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    13021 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    13081 acggcaatat ctaaaacatc aagataacga tctggatcac attcgttaac aaaacggctg
    13141 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    13201 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    13261 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    13321 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    13381 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    13441 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctagtc
    13501 gctcagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    13561 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    13621 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    13681 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    13741 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    13801 actcacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    13861 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    13921 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctat atttgcttat
    13981 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    14041 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    14101 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    14161 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    14221 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    14281 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    14341 tgattgcacc gttttaacgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    14401 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    14461 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccctgcc
    14521 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    14581 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    14641 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    14701 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    14761 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    14821 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    14881 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    14941 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    15001 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    15061 cagtccgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    15121 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    15181 atgtgggata ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    15241 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    15301 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgctct gccagaagcg
    15361 ctggcgctgg tgaaatccgg tgcactggcg ggcaccgtac tgaacgatgc taacaaccag
    15421 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gtaaaggtgc ggctgatggc
    15481 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    15541 aacctggctg aattcagcaa gaaataagac tgattgggcg cagtctatta ctgcgccctt
    15601 attgtatgac caacaaggta aaattatatg gtcagctcaa cgactccgtc ctccggggaa
    15661 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    15721 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggcgcagga
    15781 aaatcaacat tattaaaatg cttgtttggt atttatcaaa aagactccgg caccatttta
    15841 ttccagggta aagagatcga tttccattcg gccaaagaag cactggaaaa tggtatttcg
    15901 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggacaa catgtggctt
    15961 gggcgatacc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    16021 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatct
    16081 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    16141 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    16201 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaggaa
    16261 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    16321 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaac
    16381 cagcgtttcc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaacctg
    16441 acgtcactgc gccagccgtc gatccgtgat gtctcgtttg atctgcataa aggggagatc
    16501 ctcggtattg ccgggctgat gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    16561 attcgcgaga aatcggccgg caccattacg ttgcacggca aaaagatcaa taaccataat
    16621 gccaatgaag ccataaacca cggatttgct ctggtaactg aggagcgccg ctcaacggga
    16681 atttatgcct atctggatat cggttttaac tcgttaattt ccaatattcg caactacaaa
    16741 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    16801 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    16861 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    16921 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    16981 ctggcgaaga aaggcaaagg gattattatt atctcctctg aaatgcctga gttgttaggg
    17041 ataacagacc gtattctggt catgagcaac ggtctcgttt ccggaattgt cgacacaaaa
    17101 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    17161 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    17221 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    17281 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    17341 gcagggttaa ttgtcaccca ggggaccgac ctttctgcgg ggcgtcaagt agggctggcg
    17401 gcagtggtgg cagcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    17461 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    17521 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    17581 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    17641 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    17701 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    17761 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    17821 gcaaaagtat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggt
    17881 gtgttctatg ccttcggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    17941 ctcggcttta tgtatgaact ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    18001 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    18061 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    18121 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgaaatgat
    18181 tgtggtccgc attcggtcat aaacgcacat aacatcatga tctgtgtggt tttgtaggcc
    18241 ggataaggcg ttcacgccgc atccggcgac aggagccaaa tgccggatgc aacgctggcg
    18301 cgttttatcc ggcctacaat gagctcaaca tactgatttg tatggttttg taggccggat
    18361 aaggcgttca agccgcatcc ggcgacagga accaaatgcc ggatgcgtct ctggttttac
    18421 aacgttaccg ggtgttcttt ctctcccttc ttaaacttca cttctccgag atcaatacaa
    18481 cccaccgggc agacgtggcc acaaagcaga caacccacac atttctcggt attacaatgc
    18541 ggtgtgcggg ttttctcgct ccattccatc gcctggtgac cgccgtcgta acaggaaata
    18601 taacagcgtc cacagccaac acatttatca agattgatac ggggatagac aatataactg
    18661 cggtctaaat cttccgccgg gacaatattg ttattcgcca gacctaccat ctcctgcaac
    18721 gaatcaaatc cctgatcggc gaga
//
LOCUS       DAJTNA010000072        18735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTNA010000072 DAJTNA010000000
VERSION     DAJTNA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18735
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.073"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..894
                     /locus_tag="OO161_002088"
     CDS             <1..894
                     /locus_tag="OO161_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5463154.1"
                     /translation="DWFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFI
                     TIGVLGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRT
                     MWGMMLGFSAINYTAWLYLAWLPGYLQTAYYLDLKSTGLMAAIPFLFGAAGMLVNGYV
                     TDWLVKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGT
                     SCWGLIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVT
                     AAGALAYIFLVRQPINDPRKD"
     gene            complement(943..2598)
                     /gene="tsr"
                     /locus_tag="OO161_002089"
     CDS             complement(943..2598)
                     /gene="tsr"
                     /locus_tag="OO161_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5463155.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2717..3163)
                     /gene="hpaR"
                     /locus_tag="OO161_002090"
     CDS             complement(2717..3163)
                     /gene="hpaR"
                     /locus_tag="OO161_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5463156.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3435..4713
                     /locus_tag="OO161_002091"
                     /pseudo
     CDS             3435..4713
                     /locus_tag="OO161_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
     gene            4710..6176
                     /gene="hpaE"
                     /locus_tag="OO161_002092"
     CDS             4710..6176
                     /gene="hpaE"
                     /locus_tag="OO161_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5463157.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGMKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6178..7029
                     /gene="hpaD"
                     /locus_tag="OO161_002093"
     CDS             6178..7029
                     /gene="hpaD"
                     /locus_tag="OO161_002093"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5463158.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7039..7419
                     /locus_tag="OO161_002094"
     CDS             7039..7419
                     /locus_tag="OO161_002094"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5463159.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7530..8333
                     /gene="hpaH"
                     /locus_tag="OO161_002095"
     CDS             7530..8333
                     /gene="hpaH"
                     /locus_tag="OO161_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5463160.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8344..9132
                     /gene="hpaI"
                     /locus_tag="OO161_002096"
     CDS             8344..9132
                     /gene="hpaI"
                     /locus_tag="OO161_002096"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5463161.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9307..10683
                     /gene="hpaX"
                     /locus_tag="OO161_002097"
     CDS             9307..10683
                     /gene="hpaX"
                     /locus_tag="OO161_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5463162.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFSQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10693..11579
                     /gene="hpaA"
                     /locus_tag="OO161_002098"
                     /pseudo
     CDS             10693..11579
                     /gene="hpaA"
                     /locus_tag="OO161_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11830..13392
                     /gene="hpaB"
                     /locus_tag="OO161_002099"
     CDS             11830..13392
                     /gene="hpaB"
                     /locus_tag="OO161_002099"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR5463163.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13410..13922
                     /locus_tag="OO161_002100"
     CDS             13410..13922
                     /locus_tag="OO161_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5463164.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14315..16465
                     /gene="btsT"
                     /locus_tag="OO161_002101"
     CDS             14315..16465
                     /gene="btsT"
                     /locus_tag="OO161_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5463165.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGMIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16596..16799
                     /locus_tag="OO161_002102"
     CDS             16596..16799
                     /locus_tag="OO161_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5463166.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16810..17766
                     /gene="yjiA"
                     /locus_tag="OO161_002103"
     CDS             16810..17766
                     /gene="yjiA"
                     /locus_tag="OO161_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5463167.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17844..>18735)
                     /locus_tag="OO161_002104"
     CDS             complement(17844..>18735)
                     /locus_tag="OO161_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR5463168.1"
                     /translation="DRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQA
                     ATSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAV
                     PMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQE
                     VVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNR
                     GTENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLW
                     LTKQDVEAHKESQEVLHVQR"
BASE COUNT         4282 a         5184 c         4988 g         4281 t
ORIGIN      
        1 gactggttca acatcaaaga gcgcggacgc ccgatgggct tcttcaacgc agcttctacc
       61 attggcgttg ccgtaagccc accgattctg gcggcgatga tgctggtgat gggctggcgc
      121 gggatgttta ttaccattgg tgtactgggg atttttctcg ccatcggctg gtatatgctc
      181 tatcgcaacc gcgagcacgt agaactgact gccgttgagc aagcttatct caatgcaggt
      241 agcgtcaatg cccgccgaga tccgctcagt tttgccgaat ggcgcagcct gttccgtaac
      301 cgtacaatgt ggggaatgat gctcggattc agtgccatca actacactgc gtggctgtat
      361 ctggcctggc ttcctggtta cctgcaaaca gcctattacc tggatttaaa aagcacaggg
      421 ttgatggcgg ctatcccttt cctgtttggg gctgccggga tgctggtcaa cggttacgtt
      481 accgactggc tggtcaaagg gggaatggct ccgattaaaa gccgtaagat ctgcattatt
      541 gccgggatgt tctgttctgc cgcctttacg ctgatagtac cacaagcgac aacatccatg
      601 acggcggttc tgctgattgg catggcactg ttctgtattc actttgccgg aacatcctgc
      661 tggggcttga tccacgtcgc agttgcttct cgcatgactg cgtcggtggg cagtatccag
      721 aactttgcca gcttcatctg cgcctctttt gcgccgatca ttactggttt tattgttgat
      781 accacccact cattccgtct ggcactaatc atctgcggtt gcgtcaccgc agcgggggca
      841 ctggcgtaca tcttcctggt tcgtcagccg atcaacgacc cacgtaaaga ttaatctcct
      901 tatgcccgat aacattttgc ttatcgggca ttttcatggc gattaaaatg tttcccagtt
      961 ctcctcgcta tctgccacgg ccattttacg cggcgcagct ggcgtcacgg tttttaccac
     1021 agccgatgtt tcacgctgct gttgctgaat ccggaacact gccactgctt cggtcaggcg
     1081 actggcctgc tcttccagcg cggcggcggc agcggcagac tcttccacca gcgctgcgtt
     1141 ctgttgagtt acccggtcca tctcagcaac cgctaagcca acctgatcga taccacggct
     1201 ctgctcatca gaagcagaag caatttcgcc cataatgtcc gtcacgcggg tcacggcgct
     1261 gacaatctcc gccattgttt ccccggcgct ttcgaccagc gtagagccaa catccacttt
     1321 ccccaccgag tcttcaatca ggcttttaat ttcacgagcc gcctgggcgc tgcgctgagc
     1381 cagattacgc acttctcccg cgaccaccgc aaaaccgcgc ccttgctcac ccgcacgcgc
     1441 agcctcaacc gccgcgttca aagccagaat attggtctgg aaggcaatgc cgtcaattac
     1501 gctgataata tcggcgattt tctgcgaact ggtggagata tcgcgcatag tctgcaccac
     1561 gttatctacc actttgccgc cgcgttgcgc cgtttcagaa gcacttaacg ccagatggct
     1621 ggcctggcgc gcattttcgg cgttctgctt caccgttgcg gtcagttgct ccatgctggc
     1681 tgccgtctct tccagcgaag cggcctgttg ctcggtgcgc gaagagagat cgttattgcc
     1741 ggtggcgatt tcgctggcac cgctatagat ggcattggcc ccgttgcgca catcaccgac
     1801 ggtacgcatc agctctccct gcatatggcg caaactctct gccagttgcc ccatctcatt
     1861 agagccatcc acctcaatcg gtttcaccag atcgccgcct gcaatatgac gaatgctgtc
     1921 aatcaggcga ttcattggcg ctaccagcga ggctttaata ccgaaccaga cggcgaagat
     1981 gaccgccagt acgacgatca tcacgcccac cagaatccac atcgcctggc tgtaggaggc
     2041 attgttatcg ctgacggcga tatcatagag ccgatcgttt tgctccatgt aagccacata
     2101 ctgcttctcg aaaccgtcct gatatccctg ggtcggctga tcaaagaact cgttgatttt
     2161 gcctgcacct aacagttgga tcagctccgc cagcgcattg tgataaatat cgtaattacg
     2221 tttgatctct gccgctgcgg cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc
     2281 cgcccagttt ttttccgcct gtttcagcga aatactggca ctctgcatca gctcagcaac
     2341 ggttgaaccg ctaccaatat tattctgatc catcatgtag cggatacccg cgcggttgag
     2401 ggtgttacgc gtctgcaaca acgcgaccca gctgccattc agcgtggatt gctgctggcg
     2461 aatggtttgt aaaacagtga aattttcttt gtcattcttt aaggcattaa agaacagacc
     2521 gcctgatgtc agttgtaaaa ggccaaaaac ggccaaaacc agcagtaagc tggtcacgat
     2581 cttgatacgt tttaacatgg tttctctttc ctgtggaccg atgcaagatt ttcggcctgg
     2641 aaagcaaaaa ctttatgaaa aggtgtaacc cgccatccat ggaaaaacca ggcgctggtt
     2701 atcagatact aaaaagttat tcattatcgc ccgggatggc ttcttgtcgg gaattgccca
     2761 gagcaataaa ttcctctaac agatgggtta actgttgcat tttctcggca gtaaactgag
     2821 cttcaatctg ccgataggct tcttcaatct gtgtctgagc gcggttatac agcgcctgcc
     2881 cctctttggt tagcgagata tagagcttcc gctgatcatt gatcggcttt aatcgcaaca
     2941 ctaaaccgtc gcgttccatc cgcgtgagaa tcccggtcag gcttgggcgc aaaatgcagg
     3001 cgcgatacgc cagatcgtga aaatccattg atgggctttc cgccagaata cgcacgatgc
     3061 gccactgttg ctcagtcaaa ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg
     3121 cttctcgcgc ctggagcaac gcaatggtta gtgagtcgtg cattatcttt cccctgaaaa
     3181 attcaaaatc attaatatag aaacagttaa tcgtcacgtt accgggactg cgctgcccct
     3241 gcaattcctg attttatcaa aattgatgat attttattaa cttactgata tttaatattt
     3301 tattatcttt aaatgtaaat ggtttgttaa ttagatcaca tttacatcac ttacttttgc
     3361 caaactacgc aatcgggaat aaaagtaatc attaacatat taatgattaa gatgatcgaa
     3421 tccgaggagt ggtaatgaaa ggcactatct tcgccgtagc gttgaaccat cgctgccaac
     3481 ttgatgcatg gcaggaagcg ttccagcaat ccccctacaa agccccgcct aaaactgcgg
     3541 tctggtttat taaaccgcgc aatacggtga ttggttgcgg tgaaccgatt ccctttccac
     3601 agggtgaaaa ggtactgagc ggtgcgactg ttgcgctgat tgtgggaaaa acggcgacga
     3661 aagtacgtga agaagatgcg gcagagtaca tcgccggata tgcgctggct aacgacgtca
     3721 gcctgccgga agagagcttt taccgcccgg caatcaaagc aaaatgtcgt gatggattct
     3781 gccccattgg cgaaaccgtg gctctcagca atgtcgataa tctgaccata tataccgaga
     3841 tcaacgggcg tcctgccgat cactggaata ccgccgattt acaacgtaac gccgcgcagt
     3901 tgctgagcgc cctgagcgaa tttgccacgc tgaatccagg cgacgccatt ctgctcggca
     3961 cgccacaggc gcgcgtggaa atacagccag gcgagcgcgt tcgtgttctc gcagaaggtt
     4021 tcccgccgct ggaaaatccg gtagtggacg aacgtgaagt gaccacgcgc aagagcttcc
     4081 caacgcagcc acacccgcac ggcacgctgt ttgccctcgg cctgaactac gccgaccacg
     4141 ccagcaaact ggaatttaag ccaccggaag aaccgctggt gttcctgaaa gcgccaaata
     4201 ccctcactgg cgataaccag acctccgtgc gtccaaacaa tattgaatac atgcactatg
     4261 aagcggagct ggtggtagtt attggcaaac gtcagcgaag ccgatgccat ggattatgtc
     4321 gcgggctaca ccgtgtgtaa cgactacgcc attcgtgact atctggaaaa ctactaccgc
     4381 cctaacctgc gggtaaaaag ccgcgacgga ctgacgccga tgctttcaac catcgtgccg
     4441 aaagaggcga tcccggaccc gcataatctg ccccttcgca ccttcgtcaa cggcgagtta
     4501 cgccagcaag gcaccaccgc cgatctgatc ttcagcgtgc ccttcctgat cgcctactta
     4561 agtgaattta tgaccctgaa tccgggcgac atgatcgcca ccggcacacc caaaggctta
     4621 tctgacgtgg tgcctggcga tgaagtagtg gtggaagtag aaggcgtggg ccgcctggtg
     4681 aaccgaattg tgagtgagga tacagcgaaa tgaaaaaagt aaatcattgg atcaacggca
     4741 aaaatgttgc aggtaacgac tacttccaga ccaccaatcc ggcaacgggt gaagtgctgg
     4801 cggatgtggc ctctggcggt gaagcggaga tcaatcaggc ggtagcggca gcgaaagagg
     4861 cgttcccgaa atgggccaat ctgccgatga aagagcgtgc gcgcctgatg cgccgtctgg
     4921 gcgatctgat cgaccagaac gtgccagaga tcgccgcgat ggaaaccgcg gacaccggcc
     4981 tgccgatcca tcagaccaaa aatgtgttga tcccacgcgc ttcccacaac tttgaatttt
     5041 tcgcggaagt ctgccagcag atgaacggca agacctatcc ggttgacgac aagatgctca
     5101 actacacgct ggtgcagccg gtgggcgttt gtgcgctggt atcgccgtgg aacgtaccgt
     5161 ttatgaccgc cacatggaag gtcgcgccgt gtctggcgct gggcaatacc gcggtactga
     5221 aaatgtcgga actctccccg ctgaccgctg accgcctggg tgagctggcg ctggaagccg
     5281 gtattccggc aggcgtgctg aacgtggtac agggctacgg cgcaaccgca ggggatgcgc
     5341 tggttcgtca tcatgacgta cgtgccgtgt cgttcaccgg cggtacggcc accgggcgca
     5401 acatcatgaa aaacgccggg atgaaaaaat actccatgga actgggcggt aaatcgccgg
     5461 tgctgatttt tgaagatgcc gatattgaac gcgcgctgga cgccgccctg ttcaccatct
     5521 tctcgatcaa cggcgaacgc tgcaccgccg gttcacgcat ctttattcag caaagcatct
     5581 acccggaatt cgttaaacgc tttgccgaac gcgccaaccg tctgcgcgtg ggcgatccga
     5641 acgatccgaa tacccaggtt ggcgcgctta tcagccagca gcactgggaa aaagtctccg
     5701 gctatatccg tctcggcatt gaagaaggcg ccaccctgct ggcgggcggc ccggataaac
     5761 cgtctgacct gcctgcacac ctgaaaggcg gcaacttcct gcgcccaacg gtgctggcag
     5821 acgttgataa ccgtatgcga gtcgcccaag aagagatttt cgggccggtc gcctgcctgc
     5881 tgccgtttaa agacgaagct gaaggcttac gcctggcaaa cgacgtggag tacggcctcg
     5941 cgtcgtacat ctggacacag gatgtcagca aagtgttacg cctggcgcgt ggcattgaag
     6001 ctggcatggt gttcgtcaac acccagaacg tgcgtgacct gcgccagcca tttggcggcg
     6061 taaaagcctc cggcaccggg cgtgaaggcg gtgagtacag cttcgaagtg ttcgcggaaa
     6121 tgaagaacgt ctgcatttcc atgggcgacc atccaattcc gaaatgggga gtctgatatg
     6181 ggtaagttag cgttagccgc caagatcacg cacgtaccgt cgatgtatct ctctgaactg
     6241 ccagggaaaa accacggttg ccgccaaggc gcgatcgacg ggcataaaga gatcagcaag
     6301 cgttgccggg aaatgggcgt cgataccatt atcgttttcg atacccactg gctggtcaac
     6361 agtgcttatc acatcaactg tgcagaccat tttgaaggcg tctacaccag caacgagctg
     6421 ccgcatttta tccgcgatat gacctacaac tacgagggca acccggagtt ggggcagctt
     6481 attgccgatg aagccttaaa gctcggcgtg cgggcaaaag cgcacaacat tcccagcctg
     6541 aaactggaat acggcacgct ggtgccgatg cgctacatga atgaagacaa gcacttcaaa
     6601 gtggtctcca tttcggcttt ctgcacggtt cacgattttg ccgacagccg caagctgggc
     6661 gaagcgattc tgaaagcgat cgaacagtac gacggcaccg tggcggtcct tgccagcggt
     6721 tcgttatcgc accgctttat tgacgatcag cgtgcagaag aagggatgaa cagctacacc
     6781 cgcgagttcg accgccagat ggacgagcgc gtggtgaagt tgtggcgcga aggccagttc
     6841 aaagagttct gcaatatgct gccggagtac gccgactact gctacggcga aggcaatatg
     6901 cacgacacgg tgatgctgct ggggatgctc ggctgggata aatacgacgg caaggtggag
     6961 tttatcaccg agctattccc aagctctggc accggtcagg ttaacgctgt tttcccgctt
     7021 cccgcgtaag gacgttttat gccgcacttt atcgttgaat gcagtgacaa catccgcgaa
     7081 gaagccgacc tgccggggtt gttcgccaaa gtgaatccga cgctggcagc cacgggtatt
     7141 tttccgctgg cgggtattcg cagccgcgtg cattgggtcg atacctggca gatggccgac
     7201 gggcagcgtg attatgcctt cgtgcatatg acgttgaaaa tcggcgcagg tcgcagcctg
     7261 gaaagccgtc agcaggcggg tgaaatgctg tttgaactga ttaaaacgca cttcgccgcc
     7321 ctgatggaga gccgcctgct ggcgttgtcg tttgagattg aagagctgca tccgacgctg
     7381 aattttaaac aaaacaacgt gcacgcattg tttaagtagc gcgcagattg cccggtggcg
     7441 ctgcgcttac cgggcctaca aaaccccaaa ccgtacaccg taggccggat aaggcgcagc
     7501 cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac accctgatcg
     7561 cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc tcgctggatt
     7621 acccagagat caccatcgaa gacgcttacg cggtgcagcg cgaatgggtt cgcctgaaaa
     7681 tcgccgaagg ccgtacgctg aaaggccaca aaatcggtct gacctcaaaa gcaatgcagg
     7741 ccagctcgca gatcagcgaa ccagattacg gtgccctgct ggatgacatg ttcttccacg
     7801 atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg gagctggctt
     7861 tcgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtc tacaacgcca
     7921 cggactacgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac atcgatccgg
     7981 aaacccagcg cccgcgcaaa gtgttcgaca ccatttctga taacgccgcc aacgccgggg
     8041 tgatcctcgg tggtcgtccg atcaaaccag acgaactgga tctgcgttgg atctccgccc
     8101 tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggcgtg ctgaatcatc
     8161 cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta caactggaag
     8221 ccgggcaaat cattctcggt ggttcgttca cccgcccggt tccggcgcgt aagggcgaca
     8281 ccttccacgt cgattacggc aacatgggtt ccattagctg ccgctttgtt taaggagaga
     8341 acgatggaaa acagctttaa agcggcgctg aaagcaggcc gtccgcagat cggattatgg
     8401 ctgggactga gtagcagcta cagcgcagag ttactggccg gagcaggatt cgactggttg
     8461 ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca gctacaggcg
     8521 attgcgccct accccagcca gccggtagta cgtccgtcgt ggaacgatcc ggtgcaaatc
     8581 aaacaactgc tggacgtcgg cacacaaacc ttgctggtgc cgatggtaca aaacgctgac
     8641 gaagcccgtg aagcggtacg cgccacccgt tatccccccg ccggtattcg cggtgtgggc
     8701 agtgcgctgg cccgcgcctc gcgctggaat cgtattcctg attacctgca aaaagccaac
     8761 gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa cttaccgcag
     8821 attctggacg tggaaggcgt cgacggcgtg tttatcggcc cggcggatct gagcgccgat
     8881 atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga gcaggcgatc
     8941 gtgcagatcc gtgaatcggg caaagcgccg gggatcctga tcgccaatga gcaactggca
     9001 aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac caccctgctc
     9061 gcccgcgccg ctgaagcgct ggcagcacgg tttggtgcgc aggccaccgc cgtgaagccc
     9121 ggcgtgtatt aatgccggat gcgctgcact tatccggcct acactcgcac caaaccgtag
     9181 gccggataag gcgtttacgc cgcatccggc aaaaagccgt accaaatcgc ggaccggata
     9241 aggcgttacg ccgcatccgc cataaacctt gtcgtaccct acaaaaatcc cattagagga
     9301 agaaaaatga gcgacacctc acctgccata ccggagagta tcgatccggc gaatcagcat
     9361 aaagcgctga ctgccggaca acaggcggtt attaagaagc tatttcgccg cctgatcgtc
     9421 tttctgttcg tgctgtttat cttctcgttc cttgatcgca tcaacatcgg ctttgccgga
     9481 ctcacgatgg gacgcgacct cggtctgagc gccaccatgt ttggcctcgc taccaccctg
     9541 ttctatgccg cttatgtcat cttcggcatt cccagcaaca ttatgctgag tattgtcggt
     9601 gcgcggcgct ggatcgccac catcatggtg ctctggggca tcgcctctac tgccaccatg
     9661 tttgccactg gccccaccag cttgtacgta ctgcgtatac tggttggcat taccgaagcc
     9721 ggctttctgc ctggtattct gctgtattta accttctggt ttccggccta tttccgcgcc
     9781 cgtgccaacg ccttgtttat ggtagcaatg ccggtaacga cagcgttggg ctcgatcgtt
     9841 tccggctaca ttttgtcgct ggatggcgtc atggcattaa aaggctggca gtggctgttt
     9901 ttgcttgaag gcttcccgtc ggtattactc ggcgtcatgg tgtggttctg gcttgatgac
     9961 tcaccggaca aagctaagtg gctgacgaaa gaagacaaaa aatgcctgca agagatgatg
    10021 gataacgatc gtctgacgct ggttcagcca gagggagcca tcagccacca cgccatgcaa
    10081 caacgcagta tgtggcggga gatcttcact ccggtggtga tgatgtatac cctggcgtat
    10141 ttctgcctga ccaacacact tagtgcgatc agcatctgga caccgcagat cctgcaaagt
    10201 tttagtcagg gcagcagtaa tatcaccatc ggcctgctgg ccgccgtacc gcagatttgt
    10261 accattctcg ggatggtcta ctggagccgt cactcagatc gccgccagga acgaaggcat
    10321 cacaccgccc ttccttattt gttcgctgcc gcaggttggt tactggcttc ggcaactgat
    10381 cacaacatga tccagatgct ggggatcatt atggcttcga ccggatcatt cagcgcaatg
    10441 gcgattttct ggacaacacc ggatcagtcc atcagcctgc gggcacgagc gatcggtatt
    10501 gcggtgatca acgccactgg caacattggt tcagcgttaa gtccgtttat gatcggctgg
    10561 ttgaaagatt tgaccggcag ctttaacagt ggattgtggt ttgttgccgc gctgctggtg
    10621 attggtgcgg ggattatctg ggcgattcca atgcagtcct cccgtccgcg agcgaccccg
    10681 taaggaacga cgatgtgtga ccgtcagatt gccaatattg atatcagcaa agagtacgat
    10741 gaaagcctag gcacggacga tgtgcattat cagtcgttcg cccgcatggc ggcatttttt
    10801 ggccgccata tgctgccaca tcgccacgaa cagtactttc agatgcattt cctcaatagc
    10861 ggacagattg agctacagct tgacgatcat cgctactcgg tggaagcgcc cctgtttgtc
    10921 ctgacgccgc cgtcagtacc tcatgcgttt attacggagt ctgacgccga cggtcatgtg
    10981 ttgacggtac gggaagatct gatctggccc ctgctggaag ttctttatcc gggcactcgg
    11041 gaaaccttcg gcctgccggg ggcctgtcac tggcagataa acccgacgaa ctggcggcgc
    11101 tggaacacta ttggcaactg atagagcggg aatcggtgga acaactgcct agacgggaac
    11161 acaccctgac gttactggca caggcagtgt tcaccctact gctgcgtaac gcaaaactcg
    11221 acgaccatgc cgccagcggg atgcgcggag aattaaaact gttccagcgt tttaatatgc
    11281 tgattgaaag ccattttcat cagcactgga cagtaccgga ttacgctaac gaactgcata
    11341 tcaccgaatc acgactcacg gacatctgcc gtcgctttgc caaccgtccg ccaaaacggt
    11401 tgattttcga caggcagctg cgagaagcca agcggctgct gctgttttct gataacgccg
    11461 tgaacaatat tgcctggcaa ctcggtttta aggatccggc ttattttgcg cgctttttta
    11521 atcgcttagt cggttgctcg cccagtgctt atcgcgccaa aaaagtacct gtgacgtgaa
    11581 aatcccttag ccttaacggg aaaccaggca ccacctgcta ttcccctttc tccgcctgga
    11641 gcaaggggat ttagtaacgt caaaaatcgc taaaagcgac ctcgatcaca aaaccgtacc
    11701 cgacctgaaa agtaccagcc attgtcccaa aagtctcttc ccgtaatcgc ccctgcaagc
    11761 ttcaattgaa taacaaaaac aacacacaaa attaacaatc gtattccgat taatactgta
    11821 gaggtcgaca tgaaaccaga agatttccgc gccagtaccc aacgtccgtt caccggggaa
    11881 gagtatctga aaagcctgca ggatggtcgc gagatctata tctatggcga gcgagtgaaa
    11941 gacgtcacta ctcatccggc atttcgtaat gcggcagcgt ctgttgccca actgtacgac
    12001 gcgctacaca aaccggagat gcaggactct ctgtgctgga acaccgacac cggcagcggc
    12061 ggctataccc ataaattctt ccgcgtggcg aaaagtgccg acgacctgcg ccagcaacgc
    12121 gatgccatcg ctgagtggtc acgcctgagc tatggctgga tgggccgtac cccagactac
    12181 aaagctgctt tcggttgcgc actgggcgcg aatccgggct tttacggtca gttcgagcag
    12241 aacgcccgta actggtacac ccgtattcag gaaactggcc tctactttaa ccacgcgatt
    12301 gttaacccac cgatcgatcg tcatttgccg accgataaag taaaagacgt ttacatcaag
    12361 ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt tgccaccaac
    12421 tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat gggcgaaaac
    12481 ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtaaa attaatctcc
    12541 cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta cccgctctcc
    12601 agccgcttcg atgagaacga tgcgattctg gtgatggata acgtgctgat cccatgggaa
    12661 aacgtactga tctaccgcga tttcgatcgc tgccgtcgct ggacaatgga aggcggtttt
    12721 gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaattaga cttcattaca
    12781 gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg tgtacaggcc
    12841 gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga ctcgatgtgt
    12901 tctgaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc actgcaaacc
    12961 tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattatcga acgcaacgtt
    13021 accagtggcc tgatctatct cccttccagt gcccgtgacc tgaacaatcc gcagatcgac
    13081 cagtatctgg cgaagtatgt gcgcggttcg aatggtatgg atcacgtcca gcacatcaag
    13141 atcctcaaac tgatgtggga cgccattggc agcgagtttg gtggtcgcca cgaactgtat
    13201 gaaatcaact actccggtag ccaggatgag attcgcctgc agtgtctgcg ccaggcacaa
    13261 agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc ggaatacgac
    13321 cagaacggct ggactgtgcc gcacttgcac aacaacgacg atatcaacat gctggataag
    13381 ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacagcgc ctgcgctttc
    13441 gtgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag ggcgacgccg
    13501 gacaatgcgg gattacggca acggccgtct gctcggtcac ggatacacca ccatcgctga
    13561 tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac ggtaagttgt
    13621 gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg ccacttcgcg ggcatgacag
    13681 gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg ctggcgcagc
    13741 cggtgctgaa aggttcgctg gccagtcttg aaggtgagat ccgcgatgtg caggcaattg
    13801 gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca gaaggtcacg
    13861 gacttatcta ctttaaacgc cgtttccatc cagtgatgct ggaaatggaa gctgcgattt
    13921 aagctccagc cccttcacgt attcgtcgaa ggggcatttt tattgtgatc tcaatcacat
    13981 atccacccga tgattaattc ttgcccaacc gctcaccgcc attcctgacc gcttacgtcc
    14041 ctaaaataac cactcagtta tttaccttac tttacgcgcg cgtaactctg gcaacatcac
    14101 tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac ggcttcggcc
    14161 tatgattaac aaattactct atgaattttg gtttgcagga aggcagcaag tgagtgaatc
    14221 cccgggagct tacaacagta agtgacagag gtgaacgaac gcagctgccg cacctgtaag
    14281 ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac tattcaagca
    14341 cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag ttgcattacg
    14401 tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg tgtatctggt
    14461 ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg accccacgcg
    14521 cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca accgttacgt
    14581 gttgtttggt caccacttcg ccgctatcgc cggtgccggt ccgctggttg gtccggtgct
    14641 cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg tagtactggc
    14701 cggtgcggtt caggacttta tggtgctgtt tatctcctct cgccgtaacg gcgcatctct
    14761 cggtgagatg atcaaagaag agatgggacc agtaccgggg actatcgcgc tgtttggctg
    14821 tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta aagccctggc
    14881 cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc tgtttatggg
    14941 tatctacatg cgctttatcc gtccggggcg tgtaggtgaa gtttctgtta ttggtatcgt
    15001 gctgctggtt gcctctatct atttcggtgg catgattgct cacgatccgt actggggtcc
    15061 ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg cgtttgtttc
    15121 cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa ccttcctgaa
    15181 aatcggcgtt atcgtcggcc tggcgctggg tatcgtggta ctgaacccgg aactgaaaat
    15241 gcctgcaatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg ctctgttccc
    15301 gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc tgatctcttc
    15361 cggtacgacg ccgaaattgc tggctaacga aaccgacgcg cgtttcattg gctacggcgc
    15421 aatgctgatg gaatccttcg tggcgattat ggcgctggtt gctgcgtcca tcatcgaacc
    15481 gggtctttac ttcgcgatga ataccccgcc tgctggcctt ggcatcacca tgcctaacct
    15541 gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag acgttaccgc
    15601 acacgcggcg gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc agatcctgca
    15661 aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggcg cgccgacgct
    15721 ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca tgggcttctg
    15781 gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg atgcgggtac
    15841 tcgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt tcctgaaaaa
    15901 aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc tgtggggcta
    15961 cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt ggccgctgtt
    16021 cggtatttcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg ttgtgctgat
    16081 taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat ggctgcttat
    16141 ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga tggaaggctt
    16201 cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc tgacggcgca
    16261 gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag gcctgagtat
    16321 tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct ggcttgcggt
    16381 gcgtaacagc gacaaacgta ctgacaaaga aactccgtac gttccaatcc cggaaggcgg
    16441 cgtgaagatc tcttcgcacc actaaccggt tgccggatgc gctgcgcgta tccggcctac
    16501 aaagtgtcta tcgtaggccg gataaggcgt ttacgccgca tccggcaaat ccctagcccg
    16561 gaccttccgg gctttacgca tatctggaag gcaaaatgtt tggtaactta ggacaggcaa
    16621 aaaaatatct cggccaggcg gcgaagatgt tgattggcat tccagactac gacaactacg
    16681 tcgagcatat gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc
    16741 gtgaacgcca gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat
    16801 ggagagagca tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa
    16861 accaccctgc tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa
    16921 aacgaattcg gcgaagtctc cgttgatgat caactgattg gcgaccgcgc cacgcagatc
    16981 aaaacgctga ccaacggctg catctgctgt tcgcgctcca acgagctgga agacgcacta
    17041 ctcgacctgc tggataatct cgacaagggc aatattcagt tcgaccgtct ggtcattgaa
    17101 tgcaccggca tggccgatcc cggcccgatt attcagacct tcttctccca tgagatttta
    17161 tgccagcgct acctgctgga cggggtgatt gcgctggtgg atgcggtaca tgccgatgag
    17221 cagatgaacc agttcaccat cgcccagtcc caggttggct acgccgaccg tattctgctg
    17281 acgaaaaccg acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac
    17341 gcccgcgcac cggtctacac cgttacccac ggcgatatcg acctgggcct tctgttcaac
    17401 accaacggtt ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt ccactttatc
    17461 gcggacaaac aaaacgatat ttcgtcgatt gtggtggaac tggattaccc ggtagatatc
    17521 agcgaagttt cccgggtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt
    17581 tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag
    17641 cgcctctaca gcgccgactg ggaccgcccg tggggcgatg aaaaaccgca tagcacgatg
    17701 gtgtttatcg ggattcagtt gccggaggat gagattcggg cggcgtttgc gggactcaaa
    17761 aagtaaccag ccctccaaca tcacaatacc tgagacttta tcaccctcat attcctccag
    17821 aatatgaggg cattcgggct taattatctc tgaacgtgca acacttcctg cgactcctta
    17881 tgcgcctcca catcctgctt cgttaaccag agcgacttac gaccaaaatt ttcgtacatt
    17941 ttcagctgat cttcatagtg ctgatcaact gttccatcgg gagcaataaa cccactctga
    18001 ccgggtgcga ccacatccca ggcaagcacc ggacgatcgc ttgtcgttgg tgagaaaaca
    18061 atcatatcgt tttctgttcc acggttttga tactccgcct gatgacgcgt ttcttccgct
    18121 gcggcctgcg gtacaccaaa gaaattattt gcccggaacg ttaaggccat tgcaggtgtt
    18181 ttccagttac tcacattatt gccatagcgt ttggaaagag tctcccaggt atcttccagc
    18241 gcagccaaca caacctcctg ctgtggtttc ccagcaaaca gatcaaccgc ctgtgggatt
    18301 ggtgatttgt ctccctgcac cgcctcatac aaaatttttg ctccaacact tatattcagc
    18361 gaaccagttg ggccgtcctg ggttgtttcg tagccactgg cgctgtacca cttatcaaat
    18421 ggcataggta cggcagccac tacggtacgc ttcaacatac tggtcagcca aacgttcagg
    18481 atggcagagc ctggctgctg ccaggtttta ccatcatcat taagcaaatt gatgccatcc
    18541 caacgtgtta atgtatccac caactgacgg cgcggatcgc tctgtgtcaa accagatgtc
    18601 gctgcttgca gagtaggtaa aaaaagcctc aggttaagat cctgacgact ggtttggcga
    18661 ataacatccc atgcctgatc agcagttaag cgtggctttt gctcaagcag tcggtcgatc
    18721 tccgtaacgc gatct
//
LOCUS       DAJTNA010000073        18547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTNA010000073 DAJTNA010000000
VERSION     DAJTNA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18547
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.074"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..893)
                     /locus_tag="OO161_002105"
     CDS             complement(<1..893)
                     /locus_tag="OO161_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5463169.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSEIYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYW"
     gene            complement(1021..2973)
                     /gene="topB"
                     /locus_tag="OO161_002106"
     CDS             complement(1021..2973)
                     /gene="topB"
                     /locus_tag="OO161_002106"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5463170.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSAD
                     KKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(2978..4021)
                     /gene="selD"
                     /locus_tag="OO161_002107"
     CDS             complement(2978..4021)
                     /gene="selD"
                     /locus_tag="OO161_002107"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5463171.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDTFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(4138..4689)
                     /locus_tag="OO161_002108"
     CDS             complement(4138..4689)
                     /locus_tag="OO161_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5463172.1"
                     /translation="MDALELLVNRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4850..6706
                     /gene="sppA"
                     /locus_tag="OO161_002109"
     CDS             4850..6706
                     /gene="sppA"
                     /locus_tag="OO161_002109"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5463173.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6873..7889
                     /gene="ansA"
                     /locus_tag="OO161_002110"
     CDS             6873..7889
                     /gene="ansA"
                     /locus_tag="OO161_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5463174.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFIILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7900..8541
                     /gene="pncA"
                     /locus_tag="OO161_002111"
     CDS             7900..8541
                     /gene="pncA"
                     /locus_tag="OO161_002111"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5463175.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(8702..8974)
                     /locus_tag="OO161_002112"
     CDS             complement(8702..8974)
                     /locus_tag="OO161_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5463176.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(9016..9429)
                     /gene="msrB"
                     /locus_tag="OO161_002113"
     CDS             complement(9016..9429)
                     /gene="msrB"
                     /locus_tag="OO161_002113"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5463177.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            9771..10766
                     /gene="gapA"
                     /locus_tag="OO161_002114"
     CDS             9771..10766
                     /gene="gapA"
                     /locus_tag="OO161_002114"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5463178.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVSVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            10850..11734
                     /locus_tag="OO161_002115"
     CDS             10850..11734
                     /locus_tag="OO161_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5463179.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(11785..12639)
                     /gene="yeaE"
                     /locus_tag="OO161_002116"
     CDS             complement(11785..12639)
                     /gene="yeaE"
                     /locus_tag="OO161_002116"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR5463180.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(12729..13475)
                     /gene="mipA"
                     /locus_tag="OO161_002117"
     CDS             complement(12729..13475)
                     /gene="mipA"
                     /locus_tag="OO161_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5463181.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGNHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            13911..15845
                     /gene="yeaG"
                     /locus_tag="OO161_002118"
                     /pseudo
     CDS             13911..15845
                     /gene="yeaG"
                     /locus_tag="OO161_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
     gene            15958..17241
                     /locus_tag="OO161_002119"
     CDS             15958..17241
                     /locus_tag="OO161_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR5463182.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            17388..>18547
                     /locus_tag="OO161_002120"
     CDS             17388..>18547
                     /locus_tag="OO161_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HCR5463183.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDLGQIYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATRGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVS"
BASE COUNT         4654 a         4698 c         4415 g         4780 t
ORIGIN      
        1 tgccagtaaa gaaatcccat tagactattt ttttgataat cttcttcgct ttcgaacaac
       61 tcgtgcgcct ttcgagaagc aagcattata taatgccagg ccagttcttc ttcaattgtc
      121 ccgttttgaa aagctgtgct tgatatcgag atcatccatg ataattccgc cgcccatatt
      181 agcttcgccg aggatttacc ggagcgatta ttagcgcaat cagagatata gtctgaggga
      241 aaaacagcaa atttattcaa caaggcgata acctgctctg gggcttcctc catgtttgct
      301 ttaaaggtat tggctccatg gtcgccagaa agaaaatgct ccattaaggc acaataaatt
      361 tcgctatctt cgatacccca ttgattctct aaagactcgc gtcttttact tatgatatcg
      421 atcgagtcaa aaggaagcac atgatattgg aaggtatctt tgccaggttc aggctttcgc
      481 ggccagaact ccagcgtttc agaccattgc ttatgataga atcgataagg tgcgatcaat
      541 tgtagcgcct gtaacttctc gatactgagc ggctcaatac ctttagcctg ataataatgc
      601 agttgttctt tttttgcttt aaaaccggcc cgaacaataa gccccatcat aattaataga
      661 taaagaaaag agcatcccgc ggtaatcagg cctctttcat tcaaaccgtt ggatgttatc
      721 gctgcgaaca caaacattac agcgacaaca catgttaaat aaaaacccca cttacaaagc
      781 agcatggcct tattttcttt aatcatccgt tcaaaattac tattaaatat ttcccagcca
      841 ttaaaagaat acttctcgct cccaggatgg ttttgtaata aaactttttt catcacattc
      901 cctgttatta catactttag aggcgataat tttatccatg caaaaaaaat atccaacgaa
      961 ttttcttgat tcgctggata tttcagaata tgactcgaat agcacgaaag attcactcgc
     1021 ttacgctatc gccccgcttc cgacttcatc tgctggcgga ctttttttcg cactacgttt
     1081 acgcggtgca gcctttttct tatcagcact gcccggagcc acaatgccgc gaaactgccg
     1141 caccggcgta cgtttggctt gatcaataag ctgatatagc gtccccacca gcggctgcat
     1201 aaagtcctga tagcgacact gcttttcgct gatttgcgtc agcaccgatt cccagtgcgc
     1261 ggtcatgtcc ggtcgcgtcg ccatttctgg cagcgaatgg aataacgctt ttccggcgtc
     1321 ggtagagtgg atatagcgcc cttttttggt cagaaaacca cgcttgaaca gcagttcaat
     1381 aatcccggca cgggttgctt ctgtccccag accatcggtc gcacgaagga tctttttcag
     1441 atctttgtcc tgcacaaagc gcgcgatccc ggtcatcgcc gaaagcagtg ttgcatcggt
     1501 aaaatggcgc ggcggctggg tttgccgctc taccacttca cctttttcac acagcaactc
     1561 atcgcctttc gccaccacag gcaatggcgt accgtcgttt tcttcatcgc gctctttgct
     1621 gcctaacagc gtgcgccagc ctgcttcagc aagaaaacgc gctttagcga caaatttgcc
     1681 tttggcaatg tccagttcga taacacactt gcggaacacc gcatccgggc agaactgcat
     1741 caagtactga cgggcaatca ggttatagac cttcgcttcg ttctccgtca ggttgatcgc
     1801 agaactccgt gcggtcggga tgatggcgtg gtgtgcatcg acctttttgt catcccaaca
     1861 gcggttgcgt atatctggat ctaccactgg ttgcggcaac agatctggtg catgaacgct
     1921 gatggcattc atcaccgcgt ggcgtccggc aaaatgttct tctggcaaat agcgacaatc
     1981 ggaacgcgga taagtgatta gcttgtgcgt ttcgtacagc ttctggcaga tatcaagcac
     2041 gttctgcgcg ctcagaccaa agcgttttgc cgcttcaatc tgcaacgccg aaagcgaaaa
     2101 aggcagcggc gcggattctg attcccgttt atcgttatag ctggtgacaa ttgctggttg
     2161 accactaatg cggttaacca catgctccgc cagtggacga tgtaacaagc gcccttcttc
     2221 atcctggtac ggttcacacg cttcgctcgg ttgccagata gcggtaaacc gctcatcggc
     2281 aggtgtcacg atatgtgctt tgacttcaaa gaagtctttc gccacgaagt tttcaatctc
     2341 ttcatcgcgg cgcaccacca gcccgagcac gggtgtctgc acgcgtccca cggaaagtac
     2401 gccctgataa ccggcattgc gaccgagaat ggtatacgca cgggtcatat tgatgccgta
     2461 cagccagtcg gcacgcgctc gcgccagcgc agaaacgcac aatggcacaa actcactgtt
     2521 ggaccgaaga cggtcgatcg cccgctcaac cgcctgcggg ttcaggtcgt ttatcaagca
     2581 acgctgcacc tgctggcgct tttccggtgc cagttgcaga tagtccagca cttcatccac
     2641 cagcagttgc ccttcacgat ccgggtcccc ggcgtgaacg atttcgctgg cttcatgcag
     2701 gaagcgtttg atgacgttaa gttgtttggt cacggagggt cggggctgta attgccactt
     2761 ttccgggaca atcggcaaat ccgcaagatt ccagcgcgca tagcggctgt cgtaggcgtc
     2821 tggctgcgcc tgctcaagca ggtgaccgat acaccaggtc accacctgac cattaccgca
     2881 ctcgataaag ccatcgcctt tccggtgcgg tttgggcagg acatcagcaa tggcgcgcgc
     2941 cagactcggt ttttcggcaa taaacaaccg cattgaatta acgaatctca accatggcac
     3001 gaccgccgcg cgctggcacc agttcgccaa ttgccgtcag ttcaatgcca aactcggcgg
     3061 ctgtagcttt gacctcattt tctgcttccg gcatgaccgc cagcagcaaa ccgccagaag
     3121 tttgcggatc gcacagcaaa tcgcgcactt cacgcggcat ttcacccatc agatgaccgt
     3181 agctggcaaa gttacgttcg gtgccgccag gcactgcgcc caacttaatg tactcttcaa
     3241 cccccggcaa ttttgggatc gcgtcatagt caacgcgcgc ctgcacacct gcgccctgac
     3301 acatttcgct caaatggccc agcagaccaa agcccgtaac gtcggtcatc gcttttacgc
     3361 cttcgatgtt ggcaaaggac gcgcctgcga tgttcatccg gcacatcact tccgtcgcta
     3421 gtccctgatg ttctggtttc agcagtgatt ttttctcagc cgtggtaaga acgccgatcc
     3481 ccagcggttt cgtcaggaac agtttgcatc cggcttgtgc ggtactgttt ttcttcaccc
     3541 gctcggtcgg tacgatcccc gttaccgcca gaccaaaaat cggctccggc gcatcgatgg
     3601 agtgaccgcc agccagcgca atacccgcct gacggcatgc atagcgtcca ccttcggtta
     3661 cttcgcgggc aatttccggg gaaagtttgt taatcggcca gccgaggatc gcaatcgcca
     3721 taatcggttt gccccccatc gcgaaggtat cgctgatggc gttagtcgcc gcaatgcggc
     3781 caaaatcgaa aggattatca acgatcggca taaagaagtc ggtggtactg ataacgctgg
     3841 tgccattgcc cagatcgtac accgccgcat cgtcgcgggt ctcattaccc acaagcaaat
     3901 tcggatcaac aaacttcgcc tgctcactgt gcaggatggt ttccaacact tttggggaaa
     3961 ttttacagcc gcaaccagct ccgtggctgt attgggtcaa acgaatcgag ttctcgctca
     4021 tggacatctc ctgtcaatgc aatccgggta tggtaaccct cattccgtga agtgataagt
     4081 gagagtgtct gaattcctgc gcctttgctc acaatccaga cagtttcgcg acaattatca
     4141 gaaataagtt acaaacggcg tcgggtccgg gacgttaatc gacgtagatg ctttcaactg
     4201 cggcgtgccg aggtagagaa aaccgacaat tttatcctgc tcacggcaac cgaatgcttc
     4261 acgcactacc ggactttcag ttaatgcgcc actgcgccag atgccgccaa atccctgggc
     4321 aacggctgcc atttgcatcg ccatgaccgc gcatccggca gacatttcct gttcccatcg
     4381 cgggacttta tgattttctt cgcatttcgc caccaccgtg atgatgagcg gtgcgcggaa
     4441 cggcgcatta cgggctttgt cgatagcttt gtcatcacta ccggcagcaa tcgccccctg
     4501 ttccagtacg gcgctgaaac gctcgcgccc ttccccttca atcacaaaaa aatgccacgg
     4561 ttgcatggac ttatggtccg gcgcacgcat acccgcacgc aggatgtttt gcagttgttc
     4621 acccgttggc gcgggttcag ccaggcggga ggcgctacgg cgattgacca atagttcgag
     4681 tgcatccatt tgattaactc ctgtcgtgat atttattcac aaaattaaca cgagagtgga
     4741 ttttgttaca gcacagtccg caattcctgc tgacaagtgc cggttgggtc attacgataa
     4801 ccacatctat tgcgcctgtg acaggtgtga ccttaagttg ggagaataca tgcgaaccct
     4861 ttggcgattt attgccggat tttttaaatg gacgtggcgt ctgctgaatt tcgtccgtga
     4921 aatggtactt aacctgttct ttattttcct cgtactggtt ggtgtgggga tctggatgca
     4981 ggtcagtggt ggtgattcga aagaaacggc cagtcgtggc gcactgctgc tggacatttc
     5041 cggtgtgatc gtcgataaac ccgacagttc tcagcggttt agtaaattaa gccgccagct
     5101 gcttggtgcc agttccgatc gtctgcagga aaactcactg tttgatatcg tcaacaccat
     5161 tcgccaggcg aaggacgacc gcaatatcac cggtatcgtg atggatctga aaaacttcgc
     5221 aggcggcgac cagccgtcta tgcagtacat cggcaaagcc ctgaaagagt ttcgtgacag
     5281 cgggaaaccg gtttatgccg ttggcgagaa ctacagccag gggcaatatt atctcgccag
     5341 tttcgccaat aaaatttggc tgtcaccgca aggcgtggtt gatctgcacg gttttgctac
     5401 caacggtctg tactacaaat cgttgctgga taagctgaaa gtttccaccc atgtgttccg
     5461 cgtgggtacg tataaatctg ccgttgaacc gtttattcgt gatgatatgt caccggcagc
     5521 ccgcgaagct gacagccgct ggattggtga gctgtggcaa aactatctga atactgttgc
     5581 cgctaaccgg cagatccctg ctcagcaggt attccctggc gcgcaagggt tgcttgaggg
     5641 tttaaccaaa accggtggcg ataccgcgaa atatgcactg gaaaacaagc tggtcgatgc
     5701 actggcatcg agtgccgaaa tcgaaaaagc tctgaccaaa gagttcggct ggagtaagac
     5761 tgataaaaat tatcgcgcca tcagttatta cgattacgca ttgaaaacgc cggcagatac
     5821 cggtgacagc atcggtgtcg tcttcgctaa tggcgcaatt atggatggcg aggaaactca
     5881 ggggaatgtt ggcggtgata ccactgcggc acaaatccgc gacgctcgcc ttgacccgaa
     5941 agtgaaagcg attgtcctgc gtgttaatag cccaggcggc agcgttaccg cgtctgaagt
     6001 gattcgcgct gaactggcag cagcccgggc agcgggtaag cctgtggttg tatcgatggg
     6061 cggcatggcg gcatctggtg gttactggat ttccacgcca gctaattaca ttgtggctaa
     6121 ccccagcacc ctgaccggtt ctatcggtat cttcggcgtg atcaccaccg tagaaaatag
     6181 tctggattcg attggtgttc atactgatgg tgtctcaact tcaccgctgg cggatgtttc
     6241 tatcaccagg gcactgccgc cggaagcgca gcagatgatg caattaagca ttgagaatgg
     6301 ctataaacgc tttatcacgc tggttgctga tgcgcgtcat tcgacgccgg agcaaattga
     6361 taaaatcgcc cagggccacg tctggaccgg tcaggatgca aaagctaacg ggctggtcga
     6421 tagtctcggg gatttcgatg atgcggttgc caaagcagca gagctcgcaa aagtgaaaca
     6481 gtggcatctg gaatactacg ttgatgaacc gaccttcttc gacaaagtga tggacaacat
     6541 gtctggttct gtccgggcaa tgttgccaga tgcgttccag gccatgttac ctgcaccgct
     6601 tgcctcggta gcctctaccg ttaaaagtga aagcgacaag ctggccgcgt ttaacgaccc
     6661 acaaaaccgt tatgcgtttt gcctgacctg cgccaacgtg cgttaagtct tgtactgagt
     6721 ggccgacaga tcgtcggcca cattattttt tacctcgacg aatcctcttc ccgctgtctc
     6781 gccccatatt tccttatccg catagtatca ggtgcgctcc tcctgcctca cgtatatact
     6841 tttgctcttt cgatatcatt catatcaata tcatgcaaaa gaaatcaatt tacgtcgcct
     6901 acacgggcgg gaccatcggg atgcagcgtt ccgagcaggg ttatattccg gtgtcaggtc
     6961 atctacaacg ccaactggcg ctgatgccgg aattccatcg cccggagatg ccagatttca
     7021 ccattcatga atatacgccg ttgatggatt cttctgatat gacgccggat gactggcagc
     7081 atattgctga agatattaaa gcgcactatg acgactatga tggttttatc attctacacg
     7141 gtaccgacac gatggcgtat accgcctctg cgctgtcgtt catgctcgag aatctcggta
     7201 aaccggtcat tgtgacaggg tcacaaatcc cgctggctga gttacgctct gacggacaaa
     7261 ttaatctgct gaatgcgttg tacgttgcgg cgaattatcc gatcaacgaa gtaacgctct
     7321 ttttcaataa ccgattgtat cgcggcaacc gcactaccaa agcccatgcc gatggttttg
     7381 atgcgtttgc ctctccaaac cttcctccgt tactggaagc tggtatccat attcgtcgtt
     7441 tgaatacgcc acccgccccg cacggtgaag gggaattaat cgttcatcca atcacgccac
     7501 aaccaattgg cgtagtgacg atttatccgg ggatttctgc tgatgtcgtg cgcaattttc
     7561 tgcgccaacc ggtgaaagca ttgattctgc gctcctatgg cgtgggtaat gcgccacaaa
     7621 acaaagcctt cctgcaggaa ttacaagaag ccagcgatcg cggtattgtg gtggtcaacc
     7681 tgacacaatg tatgtccggt aaagtgaata tgggtggtta tgccaccggt aacgccctcg
     7741 cccatgccgg cgttattggc ggtgcagata tgactgtaga agccacgcta accaaactgc
     7801 attacctgct gagccaggaa ctggatactg aaaccattcg caaggccatg agccaaaacc
     7861 tgcgcggcga actgacgccg gatgattaag gagactgcaa tgccccatcg cgccctgtta
     7921 ctggtcgatt tacaaaatga tttctgtgct ggtggcgcgc tcgccgtacc ggaaggtgac
     7981 agtacggtgg aggttgctaa ccgccttatt gactggtgcc agtcgcgcgg agaagcggtt
     8041 atcgccagcc aggactggca tccggcgaat cacggcagtt ttgccagtca gcacggtgta
     8101 gagccttata cgccaggcca actcgacggt ttgccacaaa ccttctggcc agatcactgt
     8161 gtgcagaaca gtgaaggcgc acaattacat ccgttactga accaaaaagc gatcgcagcg
     8221 gtgttccata aaggcgaaaa tcctttagtt gacagttaca gtgccttttt tgataacggc
     8281 cgtcggcaga aaacctctct cgatgactgg ttacgcgatc atgaaatcga tgaattgatc
     8341 gttatgggcc tggctactga ctattgcgtg aagtttaccg tgctggacgc gttacagtta
     8401 ggttataagg tgaacgtgat taccgatggt tgtcgtggcg tgaatatcca gccccaggac
     8461 agtgcacacg cgtttatgga gatgtcagca gctggggcaa cgctatatac gctggcagac
     8521 tgggaagaga cacaggggta atattagttg aattcatctc tggcctacat ttcctcaatg
     8581 acattgtagg ccagataata cttgtcagtg ttgcatttaa cctacttgca gctaaaacac
     8641 gacgtccgaa caagtatatt tgccctccct taataacagg cttaaatact agataactca
     8701 ttcacttaaa catagcaata ggctttgctg agataccaaa atcttctttt aactgctgtt
     8761 tgctcttcat gaccatctga ccatttgtgt caattgtcat atgctgtgct tcggtattat
     8821 ggcgggcttg ccacagcatc accagttgca ggcaattctc tttttgttcc tcggtcaacg
     8881 caacgccatc aggccatttc cccagttcaa cggcggtcga caaacgctgg tatacctcag
     8941 gcgtcatgct gttgataatg tcatcaagat tcataatttt tccgctcctg tggaataagt
     9001 tgctgaatcg ttttttcaac cgttgatttc ttcgccgttt tcgccatcag tgaagcgtaa
     9061 agaagcagag ttaacacaat aacgttcgcc tgttggttgc ggcccgtcgg gaaagacatg
     9121 ccccagatgg gcatcacagt taccgcaacg aatttctatg cgctgcattc catgtgacaa
     9181 gtctttgata taacgaatgg attcttcact caccggttcg tagaaactgg gccagccaca
     9241 gccggaatca tacttggttt cggaatgaaa cagcggggca tcgcagatca aacagtgata
     9301 tacgccgtca cgcttgttat gcagtaaacg ccccgtaaat ggcggctctg tcccatgatt
     9361 ctgcgtcacg taaaactgca tctcggacaa attttttttc agttcttctg ccgaaggttt
     9421 attagccatt tgctcacatc tcactttaat cgtgctcaca ttacgtgact gattctaaca
     9481 aaacattaac accaactggc aaaattttgt cctaaacttg atctcgacga aatggctgca
     9541 cctaaatcgt gatgaaaatc acatttttat cgtaattgcc ctttaaaatt cggggcgccg
     9601 accccatgtg gtttcaagcc caaaggaaga gtgaggcgag tcagtcgcgt aatgcttagg
     9661 cacaggattg atttgtcgca atgattgaca cgattccgct tgacgctgcg taaggttttt
     9721 gtaattttac aggcaacctt ttattcacta acaaatagct ggtggaatat atgactatca
     9781 aagtaggtat caacggtttt ggccgtatcg gtcgcattgt tttccgtgct gctcagaaac
     9841 gttctgacat cgagatcgtt gcaatcaacg acctgttaga cgctgattac atggcataca
     9901 tgctgaaata tgactccact cacggccgtt tcgacggtac cgttgaagtg aaagacggtc
     9961 atctgatcgt taacggtaaa aaaatccgtg ttaccgctga acgtgatccg gctaacctga
    10021 aatgggacga agttagtgtt gacgttgtcg ctgaagcaac tggtctgttc ctgactgacg
    10081 aaactgctcg taaacacatc accgctggtg cgaagaaagt ggttatgact ggtccgtcta
    10141 aagacaacac tccgatgttc gttaaaggcg ctaacttcga caaatatgct ggccaggaca
    10201 tcgtttccaa cgcttcctgc accaccaact gcctggctcc gctggctaaa gttatcaacg
    10261 ataacttcgg catcatcgaa ggtctgatga ccaccgttca cgctactacc gctactcaga
    10321 aaaccgttga tggcccgtct cacaaagact ggcgcggcgg ccgcggcgct tcccagaaca
    10381 tcatcccgtc ctctaccggt gctgctaaag ctgtaggtaa agtactgcca gaactgaatg
    10441 gcaaactgac tggtatggcg ttccgcgttc cgaccccgaa cgtatccgta gttgacctga
    10501 ccgttcgtct ggaaaaagct gcaacttacg agcagatcaa agctgccgtt aaagccgctg
    10561 ctgaaggcga aatgaaaggc gttctgggct acaccgaaga tgacgtagta tctaccgatt
    10621 tcaacggcga agtttgcact tccgtgttcg atgctaaagc tggtatcgct ctgaacgaca
    10681 acttcgtgaa actggtatcc tggtacgaca acgaaaccgg ttactccaac aaagttctgg
    10741 acctgatcgc tcacatctcc aaataagttg agatgaaact gtgatctaaa aagagcgact
    10801 tcggtcgctc ttttttttac ctgataaaat gaagttaaag gactgcgtca tgattaagaa
    10861 aatttttgcc cttccggtca tcgaacaaat ctcccctgtc ctctcccgtc gtaaactgga
    10921 tgaactggac ctcattgtgg tcgatcatcc ccaggtaaaa gcctcttttg cattacaggg
    10981 cgcacacctt ctctcgtgga aacctgcggg tgaagaagaa gttctgtggt tgagcaacaa
    11041 cacaccgttc aaaaatggcg tcgctattcg cggtggcgta ccggtttgct ggccgtggtt
    11101 tggtccggcg gcacaacaag gtctgcctgc gcacggtttt gcccgcaacc tgccgtggac
    11161 gctgaaatca catcatgaag atgctgatgg cgtagcgctg acttttgaat tgacgcaaag
    11221 cgaagagacg aaaaaattct gtccgcacga ctttacgctg ttagcgcatt tccgcgtggg
    11281 taaaacctgt gaaatcgatc ttgaatcaca tggcgaattt gaaaccacct ctgccctgca
    11341 tacctacttt aacgtgggtg atatcgctaa ggtaagcgtc agtgggctgg gcgatcgctt
    11401 cattgataaa gtgaatgacg cgaaagaaga tgtactgacc gacggtattc agaccttccc
    11461 tgaccgtacc gatcgcgtgt atctgaatcc gcaagattgc agcgtgatta atgatgaagc
    11521 gctgaatcgt attatcgccg taggccacca gcatcatctg aacgttgtag gctggaaccc
    11581 gggaccggcg ctttcaatta gcatgggcga tatgccggat gatggctaca aaacatttgt
    11641 ttgtgtagaa acggcttacg cttcagaaac gcaaaaagtc accaaagaga aacctgcaca
    11701 tctggcgcaa tccattcgcg ttgcgaaacg ttaatttgcg ttaatgttgt gtgccgggtg
    11761 cgatgcatcc ggcacgtatc aacatcacac catatccagc gcagtttttc cttttggtgc
    11821 cggatacgct ttatccagca tggttaattc cgctgacgaa agttcgacct caagcacagc
    11881 cgcattctgt tgaacatggg caatcgtggc cgcttttgga atcgccatca caccctgatg
    11941 gctgattacc ctcgccaaca atacttgtgc cgagctgata ttgtgagcat gtgcaatttc
    12001 gttgactacc gcgtttttta acagtccatt gcgcaaccgc ccggcctggg ctaatggact
    12061 gtaagccatc accggcatct gctgttgctg gcaccagggg agtagatcgt actcaatccc
    12121 tcgtgaaccg agatggtaaa gcacctgatt agtggcacac tgatttcccc ccggcagctg
    12181 ccagagttcc tgcatatcag cataatcaag gttagaaacg ccccagcggc ggatttttcc
    12241 ctgggcgatc aatttttcca tcgctgcaac agtctcttca aaagcgaaac tgccagacca
    12301 gtgtaataag tagagatcga gataatcagt attgagacgg cgtaaactgg cttcgcatgc
    12361 atttatcgct ttttgcctgc cagcattcca cggatagact ttagagacga gaaagacgtt
    12421 atcacgcaga ccggttaatg cttccccaac caccttttcg gcaccgccat cggcatacat
    12481 ttcggcggta tcaatgaggg ttaaaccgag ctcaatgccc gcgcgtagtg cagcaacttc
    12541 tgttttgcgc tgactggcat cttcgcccat ataccatgtt ccctgcccta tggctggcag
    12601 tgagacatcg ccactaaatt gaatcatttt ttgttgcatt gtttcctccc aggtattgca
    12661 ccaccgtaat gcaaaacagg gcgtgacgcc ctgtttttat gcacaaaatg ccctggaaag
    12721 atgcattatc agaatttgta ggtgatcccg gtagaaatca ggccagtcca ggatttatcc
    12781 accatcgggc tgtcagtaac ttcatcagac agacgggtat agcgcgcggt accgtaaaca
    12841 ctccagtcgc cgaggaagtt gtagctggcg ctcagctcca ggtaagggct ccagctgtcg
    12901 ttcgggttat agccacgcag accgctgcga gcggactctt tgcgcgatac gccataatag
    12961 tattcgttct ggttttcgct gttccactgc acaccaatac ccggagtcac ggtcaggcca
    13021 ccgttggtgt aacgatacaa ccaggccata tcccagacaa tgccgttgct gttatccagg
    13081 gtatcgccag ccagggtggt acgcaggtaa ccgtactggg taaagtgagc ataagacaga
    13141 ccagccatca tggtgctctt acggtcatcc aggtgacgca tttggtgatt gccactgtct
    13201 ttcgctttga agtaaagcgg cgaccagtaa gcggtaattg aaagtttatc cgttgcgtca
    13261 ttccacaggt agtaaccacc acctaagcca cggaaccaga agttatcgcc ttcatagttg
    13321 attaccggta ctgggtaaac atcggtatcg taatctttat atgggtgctc aacgacacct
    13381 acgcctgcgc ccaaggaaaa tttaccttca gcgtgcgcta cgcctgcaga cgttgcgata
    13441 agcactccaa gtgccagaag tttgagtttg gtcacaatta atcattcctt aaacaaatgt
    13501 ttagcgggcg acaaagttta cccgtcaata catcgaaacc caaccttttt acgttttcat
    13561 tttttaaagt aactgtttaa ttttcctgcc gcggatgaca ccgcgcttac agccaaatga
    13621 atttagcgtt actggcgagc ctggtcttta cattaattat gcaaaattta tggatgagtt
    13681 gttgatatgc cattgaaatt aagaaagccg tgcaggcaag ttttccattt gccatctacg
    13741 cttaattttg aaggtgtatc accgggcacg ttgttctcat cgtcgataaa atggcatgag
    13801 agttgctgtg ttttagcaag agacgtcgtt cagtttacct cttccgggag cctctactat
    13861 tcatatgaac ggctcttaac ctgtgctaaa aaacgaaagg acggcatacc atgaatatat
    13921 tcgatcacta tcgccagcga tatgaagctg ccaaggacga agagttcaca ctgcaggagt
    13981 ttcttaccac ttgtcggcaa gatcgcagtg cttatgccaa cgcggctgag cggctattga
    14041 tggctatcgg tgagcctgtc atggtcgata cagcccagga acccagactt tctcgactct
    14101 tttctaaccg ggtcattgca cgttatccgg cgtttgaaga gttttacggc atggaagacg
    14161 cgattgaaca gattgtctct tatctgaaac acgcggctca ggggctggaa gagaagaaac
    14221 aaatcctgta tctgctgggg cctgtgggtg ggggtaaatc atcgcttgct gagcgactga
    14281 aatcattaat gcagctcgta ccgatttatg tattgagcgc caacggtgag cgtagcccgg
    14341 tcaacgatca tccgttctgt cttttcaatc cgcaggaaga tgcgcagatt ctggaaaaag
    14401 agtatggcat tcctcgccgt tatctcggca ccatcatgtc gccgtgggcg gcaaaacgcc
    14461 tgcatgaatt tggtggcgat atcactaagt tccgggtagt gaaggtctgg ccgtcaattc
    14521 tgcaacaaat tgctatcgcc aaaacggaac ccggcgatga gaacaaccag gacatctccg
    14581 cgctggttgg gaaaatcgat attcgtaaac tcgaacacta cgcgcagaat gacccggacg
    14641 cctacgacta ttccggtgcg ctgtgccgcg ccaatcaggg gatcatggaa ttcgttgaga
    14701 tgtttaaagc accgataaaa gtgctgcatc ccttgttaac cgccactcag gaaggtaact
    14761 acaacgggac ggaaggtatc tccgccctgc cgttcaacgg gattattctc gcccactcga
    14821 acgagtccga atgggtcact ttccgtaata acaaaaacaa cgaagccttc ctcgaccgtg
    14881 tttacatcgt gaaggtgccg tattgcttgc gcatttccga agagatcaaa atctacgaga
    14941 aattgcttaa tcacagtgaa ttgactcacg ccccatgcgc ccctggcacg ctggaaacac
    15001 tgtcacgttt ttccattctt tcgcgcctga aagagccaga aaactccagc atttattcaa
    15061 agatgcgggt ttatgatggc gaaagtctga aagacaccga tcccaaagcc aagtcgtatc
    15121 aggaatatcg tgactacgcc ggtgtcgatg aagggatgaa cggtctgtcg acgcgttttg
    15181 cgtttaagat cctctcccgc gtgttcaact tcgatcatgt agaagtggcg gcaaacccgg
    15241 tccatctgtt ctacgtcctg gaacagcaga tcgagcgcga gcagttcccg caagagcagg
    15301 cagaacgcta tctggagttc ctgaaaggtt atctgatccc gaaatatgcc gagtttatcg
    15361 gcaaagagat ccagacggcc tacctttaat cctattccga atatgggcaa aacattttcg
    15421 accgttatgt tacctacgcg gatttctgga ttcaggatca ggagtatcgc gatccggata
    15481 ccgggcagct gtttgaccgc gagtctctta acgccgagct ggagaaaatc gagaaaccgg
    15541 cggggatcag taatccaaaa gatttccgca acgagattgt taacttcgta ctgcgcgcca
    15601 gagcgaataa cagcggacgc aatccgaact ggaccagcta tgaaaaactg cgcacggtta
    15661 tcgagaagaa aatgttctcc aataccgagg agctgttgcc ggttatctcg tttaacgcca
    15721 aaacgtcaac cgacgagcag aagaaacacg acgactttgt cgaccgtatg atggaaaaag
    15781 gctacacccg taaacaggtg cgtttactgt gcgaatggta tttgcgcgta cgtaaatcgt
    15841 cttaacaacc ctggcccggt acacatgcta ccgggcctac aacgacagcg aaccgtgggc
    15901 cagagaaatg gcaacacagg cgtagcatac agttggcaaa tgtagtacgg ggggcatatg
    15961 acctggttta ttgaccggcg tctgaacggc aaaaacaaaa gcatggtgaa tcgccagcgt
    16021 tttttacgcc gttataaagc gcaaattaaa cagtcgatct ccgaggccat gaataagcgt
    16081 tcggtgactg acgtcgacag cggcgaatcc gtatccattc ccacggaaga tattagcgaa
    16141 ccgatgtttc atcaggggcg tggcggcctg cgccaccgcg tacatccggg caatgaccat
    16201 ttcgtccaga acgaccgaat tgaacgtccc cagggtggcg gcggaggttc cggcagtggt
    16261 cagggccagg ccagccagga tggtgaaggt caggatgaat ttgtctttca gatttcgaaa
    16321 gatgagtatc ttgatctgct ctttgaagat ttggccttac cgaatctgaa acaaaaccaa
    16381 caacgccagc tgaccgaata taaaacgcat cgggcgggtt ataccgctaa cggcgttcca
    16441 gccaatatca gcgttgtgcg ttccttgcag aactcactgg cgcgacgcac agccatgacg
    16501 gcgggcaagc gacgggaact tcatgcgctg gaagagaatt tggccatcat cagcaacagt
    16561 gaacctgcgc aactgctgga agaggaacgt ctgcgcaaag aaattgcaga attacgtgcc
    16621 aaaattgaac gcgtcccttt tattgacacc ttcgatttac gttacaagaa ctacgagaag
    16681 cggcccgatc cctccagcca ggcagtgatg ttttgcctga tggacgtttc cggttcaatg
    16741 gatcaatcca ctaaagatat ggctaagcgt ttttatattc tgctgtatct gttcctcagc
    16801 agaacgtata agaacgtgga agtcgtatac atccgccatc atacccaagc gaaagaagtc
    16861 gatgaacatg agtttttcta ctcgcaggaa acaggcggca ccattgtttc cagcgccctg
    16921 aaactgatgg atgaggtagt gaaagagcgt tataacccgg cacagtggaa tatttacgct
    16981 gcacaagcat cggacggcga taactgggcc gatgactctc cgctttgcca tgaaatcctg
    17041 gcgaaaaaat tattacctgt tgttcgttat tacagctata tcgaaattac ccgtcgtgca
    17101 catcagacat tgtggcgaga atatgagcat ctgcaatcta ctttcgacaa ctttgcgatg
    17161 cagcacatcc gcgaccagga tgatatttat ccggtgttcc gtgaactgtt tcataaacaa
    17221 aatgcaacag ctaaagacta aaactatcag ccaggtcatt atcgcctggc tgatttttag
    17281 cttactgtaa tttatctcat ttattacata cattagctta caatcgcttt aaatatgaca
    17341 gcataacctt tacataattt agttccagaa aacaatcatt cggaaaaatg attcagtcaa
    17401 cacgtatttc catggggtta ttctttaaat attttttatc gttaacgaaa attgatcttg
    17461 gtcaaatcta tatatctctg ccatcaataa aatccagcac tcacattgct ctccttttta
    17521 tggtttctat gggtacacaa aaattaaaag ctcaaagctt ttttattttc agtttattgc
    17581 tgacattaat tttattttgc attactacct tatataacga aaacacaaat gtaaaactca
    17641 tcccacagat gaattacctg atggttgttg tggctttgtt tttccttaac gccgtcattt
    17701 ttcttttcat gttaatgaaa tatttcacta acaaacaaat tttaccaaca ctcattttaa
    17761 gccttgcatt cttaagtggc cttatctatt tagttgaaac cattgtaatt atccataaac
    17821 caattaacgg cagtacactg atccagacaa agtcgaatga tgtttctatt ttctatattt
    17881 tccgccaact cagttttatt tgtttaacct cgctggcact cttttgttat ggaaaagaca
    17941 acatccttga caacaataag aaaaaaacgg gaatcctgtt gctggcgttg attccttttt
    18001 tagtttttcc ccttctggca cacaatctga gcagttataa cgctgactat tctttgtatg
    18061 tcgtcgacta ctgtccggac aaccatactg cgaccagggg aatcaactat acaaaaatat
    18121 tggtttgtct gtgggcattt ttactgttct ttattatcat gcgtacacga ttagccagcg
    18181 aactatggcc gttaatagca ttattatgtc tggcatcgct atgctgcaac ttacttctac
    18241 tgactctgga tgagtataat tacaccatct ggtatatcag tcgcgggatt gaagtttcca
    18301 gtaaactgtt tgttgtgtct tttctgattt ataacatttt tcaggagctg caactctcca
    18361 gcaaactggc agttcatgat gtgctgacca atatttataa tcggcgctac tttttcaaca
    18421 gcgtagagtc attattgtcg cgacctgttg ttaaggactt ctgtgtcatg ctggttgata
    18481 ttaatcagtt caaacgcatc aatgcccaat ggggacatcg tgtgggtgat aaagtgctgg
    18541 tttcaat
//
LOCUS       DAJTNA010000074        18518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTNA010000074 DAJTNA010000000
VERSION     DAJTNA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18518
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.075"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..149
                     /locus_tag="OO161_002121"
     CDS             <1..149
                     /locus_tag="OO161_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463184.1"
                     /translation="FDIGVLLSNREFTEDAIKMRKYEPYLLNDNSILSRIALLELGII
                     GEQQ"
     gene            146..334
                     /locus_tag="OO161_002122"
     CDS             146..334
                     /locus_tag="OO161_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR5463185.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            430..2904
                     /locus_tag="OO161_002123"
     CDS             430..2904
                     /locus_tag="OO161_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5463186.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPM
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     NAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASA
                     RVNQQNTENSAGKINPVTAAICREYKQTWKTLDEELAYALWPGDIDTGNIDGSIHRWA
                     KNEVIDKDREDWKRISASMRKQPDAVRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDDEGTNQGTAGTLPSPVPETDAVETAMPDNEKTECEVEDEPSVEREGPFY
                     FLFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTIA
                     QPEPVKVTADEVNKIMQAANISQPDANNLLAVSRGEFVAGISDPNDPKWVKGIETRDS
                     VNQNQQETEQNGQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDC
                     GAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADH
                     SIKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTI
                     DLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVW
                     GNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHN
                     ALDDARYQAKYVSVIWQKLIPNQADF"
     gene            2969..3217
                     /locus_tag="OO161_002124"
     CDS             2969..3217
                     /locus_tag="OO161_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5463187.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            3195..4325
                     /locus_tag="OO161_002125"
     CDS             3195..4325
                     /locus_tag="OO161_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5463188.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMRSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDVITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(4371..5009)
                     /gene="ompW"
                     /locus_tag="OO161_002126"
     CDS             complement(4371..5009)
                     /gene="ompW"
                     /locus_tag="OO161_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5463189.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            5249..5338
                     /locus_tag="OO161_002127"
                     /pseudo
     CDS             5249..5338
                     /locus_tag="OO161_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5366..6109
                     /locus_tag="OO161_002128"
     CDS             5366..6109
                     /locus_tag="OO161_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5463190.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            6139..6678
                     /locus_tag="OO161_002129"
     CDS             6139..6678
                     /locus_tag="OO161_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5463191.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            6783..7181
                     /gene="yciA"
                     /locus_tag="OO161_002130"
     CDS             6783..7181
                     /gene="yciA"
                     /locus_tag="OO161_002130"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5463192.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(7221..7949)
                     /gene="tonB"
                     /locus_tag="OO161_002131"
     CDS             complement(7221..7949)
                     /gene="tonB"
                     /locus_tag="OO161_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5463193.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            8164..8460
                     /locus_tag="OO161_002132"
     CDS             8164..8460
                     /locus_tag="OO161_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5463194.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            8760..10013
                     /gene="kch"
                     /locus_tag="OO161_002133"
     CDS             8760..10013
                     /gene="kch"
                     /locus_tag="OO161_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5463195.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(10068..10241)
                     /locus_tag="OO161_002134"
     CDS             complement(10068..10241)
                     /locus_tag="OO161_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5463196.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            10384..11844
                     /gene="cls"
                     /locus_tag="OO161_002135"
     CDS             10384..11844
                     /gene="cls"
                     /locus_tag="OO161_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5463197.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            11879..12208
                     /locus_tag="OO161_002136"
     CDS             11879..12208
                     /locus_tag="OO161_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5463198.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(12261..13265)
                     /gene="oppF"
                     /locus_tag="OO161_002137"
     CDS             complement(12261..13265)
                     /gene="oppF"
                     /locus_tag="OO161_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5463199.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(13262..14275)
                     /gene="oppD"
                     /locus_tag="OO161_002138"
     CDS             complement(13262..14275)
                     /gene="oppD"
                     /locus_tag="OO161_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5463200.1"
                     /translation="MSVIETATVPLAQQQAGALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGHLCACFKPVE
                     ELL"
     gene            complement(14287..15195)
                     /gene="oppC"
                     /locus_tag="OO161_002139"
     CDS             complement(14287..15195)
                     /gene="oppC"
                     /locus_tag="OO161_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5463201.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSTPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVISHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(15210..16130)
                     /gene="oppB"
                     /locus_tag="OO161_002140"
     CDS             complement(15210..16130)
                     /gene="oppB"
                     /locus_tag="OO161_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5463202.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPIRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(16216..17847)
                     /gene="oppA"
                     /locus_tag="OO161_002141"
     CDS             complement(16216..17847)
                     /gene="oppA"
                     /locus_tag="OO161_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5463203.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            18131..18427
                     /locus_tag="OO161_002142"
     CDS             18131..18427
                     /locus_tag="OO161_002142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463204.1"
                     /translation="MRFLLANTFCKIVKLFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSFSIEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
BASE COUNT         4904 a         4470 c         4385 g         4759 t
ORIGIN      
        1 gttttgatat tggtgttctg ctcagtaacc gggagtttac ggaagatgcc attaagatga
       61 gaaaatatga accttatctt ctcaatgata attccatact ttccagaatt gcccttcttg
      121 aacttggtat tatcggagaa cagcagtgac ttcagcattt gcactggtga tgaccgtttt
      181 tcttataacg ggtgagccac aaaatgtgat taccggaatt tatgacagta agtcatcctg
      241 cattcaggta agggacgaac aaaaaatccc cggtgaatgc ctcccgttaa aaaaagtatc
      301 gctgaacctg aataacgaaa taccggctgg ataacccgcc agccatatta acgccatacc
      361 aacggattaa aaatgccagc aatggcaggg attcgttcac cctgaaatct gtaatgaggt
      421 taaaacaaaa tgagtaaagt ctttatttgc gccgccattc cggacgaaca ggcaataaag
      481 gaagaaggtg ccgtcgctgt agccactgcc attgaagccg gtgatgaacg tcgcgcccgc
      541 gcaaaatttc actggcaatt cctggaacat tatccggctg ctcaggactg cgcttataaa
      601 tttcttgtct gcgaggataa acccggtata ccccgccctg ccctcgattc ctgggatgct
      661 gaatatatgc aggaaaaccg ctgggatgag gaatccgctt cctttattcc ggtcgaacca
      721 gaatccgatc cgatgaacgt caattttgac aagctgtccc ttgaagtaca gaacgcggtc
      781 ctggttaagt tcggtacatg tgaaaacatc accgttgata tggcgattga cgcgcaggaa
      841 ttactgcagg aagacgttgc tacctttgac gggcatatcg ttgaagcact gatgaaaacg
      901 cctgaaatta acgctatgta tccggaacgc aaactgttcg ctatcggatg ggttaaacac
      961 aaatgtaatc cgggtgccaa atggcccgaa attcaggctg aattacgtaa ctggaaaaaa
     1021 cggcaggacg cagagcgcaa agagactgga aaatacacgt ctgttgttga tctcgccagc
     1081 gccagagtca atcaacagaa cactgaaaac tcagcaggaa aaatcaaccc agtcactgcc
     1141 gccatttgtc gcgaatacaa gcagacatgg aaaacgctgg atgaagaact ggcctacgct
     1201 ctctggcctg gcgatattga taccggaaac attgacggca gcatccatcg ctgggcaaaa
     1261 aatgaagtta tcgacaaaga tcgcgaagac tggaagcgca tttccgcatc aatgcgcaaa
     1321 caacccgatg ccgttcgcta cgaccgtcag actatttttg gccttgtccg tgagcgtccg
     1381 atcgacattc acaaagatcc cgtagcactg aacaaataca tcactgaata cctgactacc
     1441 aagggcgtgt ttgaagatga cgaaggaaca aatcagggca cagctggtac tctcccgtca
     1501 ccagtaccag aaactgatgc agtggaaacg gcaatgccgg acaacgaaaa aaccgaatgc
     1561 gaagtggaag acgaaccatc tgtagagcgt gagggaccgt tctacttcct tttcaccgat
     1621 aaggacggcg aaaaatacgg tcgcgcaaac aaactttctg gtctggaaaa agcactggcc
     1681 ctgggagcta cggaaatcac aaaagaggaa tacttcgcac gtaaaaacgg cacgtactca
     1741 ggttcacaac aaaatactgg tgcatctgac acgatcgcac aaccagagcc ggtaaaagtt
     1801 accgctgacg aagtaaacaa aattatgcag gcagccaata tcagccagcc tgacgccaat
     1861 aatttgcttg ctgtatcacg tggtgaattt gttgcaggga ttagcgaccc gaatgatccg
     1921 aaatgggtga aggggattga aacccgcgat tcagtgaatc agaaccagca agaaacggaa
     1981 cagaacggcc agaaagcgga acaaaacagc ccaaatgcgt tacaaaacga gccagaaacg
     2041 aaacagcctg aaccagtggc gcaacaggaa gtggaaaaag tctgcaccgc ctgcggtcag
     2101 accggcggcg gcaactgccc tgattgtggc gcggtgatgg gcgacgcaac ataccaggaa
     2161 acattcgatg aagagtatca ggttgaagtt caggaagatg atccggagga aatggaaggc
     2221 gctgaacatc cacacaagga gaacactgac ggcaatcagc atcacgatag cgataatgaa
     2281 actggcgaga cggcagatca ctcaattaag gtgaacggtc atcaagaaat cacatccacc
     2341 agcaggacgt gtgaccatct aatgatcgac cttgaaacca tgggaaaaaa tcctgatgcc
     2401 ccgatcatct caataggtgc aatatttttc gatccgcaaa ccggagatat gggaccggaa
     2461 tttagtaaga ctatcgatct ggaaactgct ggcggggtca ttgatcggga caccattaaa
     2521 tggtggctta agcaatcacg cgaagcgcaa tctgccatta tgaccgatga aatcccgtta
     2581 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
     2641 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
     2701 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
     2761 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
     2821 aatgcacttg atgacgcccg ttaccaggca aaatacgttt cagttatctg gcaaaaactg
     2881 atcccgaatc aggctgattt ttaatgttca accccggtcg ttgcccacca gctatagtgg
     2941 cggcgaccat gattagcgaa cgacgctcat ggcaagactt attctgctca ctgagtgggc
     3001 aaaagaggaa ttcagtgaac cggtcccaac tccgagtacg ttaagtaaat acgctaaagc
     3061 cggaatgata tttcctctcc ccaaaaaagt tggaagacgc tggcgagtgg atccgcaagc
     3121 tcgctttgtc ggaatggtaa acaagccgga ggtgatcgcc acagatcacc ctgctttgaa
     3181 gaggatactg gaagatggcg cgcccgcgaa aatataaaac cgatgttccg ggattatctc
     3241 cgtattttga taaaagaaat aacaaagttt actggcgtta caggcatccc ataacaggca
     3301 aaaatcacgg tctcggcagt attgaccaga aactggcaga aactattgca gcagaagcga
     3361 acagccgtct tgcccggcag caaatggaac aaatgcgcag tctgcaggag aaaattatta
     3421 gtgataccgg cggttcatca accgttacca tttttctgaa taattacaga aaaattcaac
     3481 aggaaagata tgaaaacggc gagatcaaac tcaacacgct gaaacagaaa gcggcccctc
     3541 tcagggtatt tgatgaacgt tttggcacca gaccgttaga tgtcataacc gtaaaagatg
     3601 tggtatcagt actggaagag tacaaggcca gaggacataa cagaatggga caaattttca
     3661 ggaaagtact gatcgatgtt ttccgggaag ctcagcaaac gggcgatgtc ccgccaggct
     3721 ttaaccctgc agaatcggca aaaaaaccgc aggtgcggat atcaagacag cgactgactt
     3781 ttgatgagtg gatgatgatt tataacgcag cggaaaagga tggttacttt ttacagcgcg
     3841 gtatgctgct ggcactgatg acaggccagc gcctttcaga tatttgcaaa atgcaatttt
     3901 cggatatccg ggatggttat cttcatgtcg aacagcaaaa aacaggaacc cggattgcca
     3961 tccctctggc tctgcgttgc gataaattaa atctcaccct ggatgatgtg gtgtcatcct
     4021 gccgcgattg cgttcttagt ccgtggctat tgcaccacca tcacgcgaaa gggacagcta
     4081 agcgcggcgg gatggttaag ccagcaacat taaccgttgc atttaaaaaa gcccgggatt
     4141 ctgtggatta caactggcgt gctaatggca cccctccctc tttccatgag cagagatctt
     4201 tatcagagcg attgttcaga gagcaggggg ttgataccaa aattttgcta ggccattcga
     4261 atcaaaaaat gaccgatatt tacaacgacg cacgcggtaa ggaatggaaa aaactggtca
     4321 tttgatgacc agttttgcag aggggttttg cagaggtttt gcagagaaaa ttaaaaacga
     4381 tatcctgcgg agaacataaa cacccacgga tccagtcgta ccgagtcttt tacggtagta
     4441 acaccagatt tgtacttagc cgtggtatcg atatccatgt accacactga catgttaacc
     4501 aaccagtcac ggttaatcag ataatcaacc cccacctgcc cggcagctcc ccaggaatct
     4561 ttcagactga gatcggaaag ccctgcctct ttgccatgat cgttaaatcc attatcaaag
     4621 aaggtggtgt agttaatacc tgccccaacg taaggacgga atttgctgct ggcatcacca
     4681 aaataccact gcgccatcag tgttggcggc agatggtgaa cggttgcaat atcgccggtc
     4741 gcccgggtgc cgattttatg gcggaacggc gtcgctgcca gtaattccac accaatgttg
     4801 tcggtcgcca tataagtaaa cgtcaagccc agttgagtgt tattggtcac gctgaatcca
     4861 cccagacttc ctaacgtacc accagcacct tctgttggac gtacggttgc agaacctgca
     4921 cgcataaaaa attcgcctgc ttcatgcgca aaggcactgc cagagagaag agttgttact
     4981 gccaaagccg ccactgttaa ctttttcata tccgctccgt cgttatggtt ataaaaacgt
     5041 gacgaatata cctacattgg agtaacaaat gatcctacat agatcacatt acatatgata
     5101 attcaccctt tattgatctg gattaattta gaagttgcat tgagaatcct ggttaacgtg
     5161 atttaaatca atttttaaat attttcgcta cgcaaatatt aactcgatcg ccacttgccg
     5221 cggtttgccc acgcctgaag gccagcaatt acctccgctt tacaaagata tgcttttcca
     5281 tacaagacct gatgctgcca cccgaggctt aaccagggta caattgcccg ctaattaaca
     5341 cctgcataaa actcaaggag agtgcatgtc tatcacggcg cagtccgtat accgtgacac
     5401 cggaaatttc ttccgtaatc aatttatgac cattctgttg gtatcgttgc tatgtgcgtt
     5461 tatcacagtg gtgttagggc atgttttctc acccagtgat gcacagcttg cgcagctcaa
     5521 tgacggcgtg cccgttagcg gcagtagtgg gttgttcgac ctggttcaga atatgtcacc
     5581 ggaacagcaa caaattttgc tgcaggcttc agcggcgtcc actttttcag gattaatcgg
     5641 taacgccatt ctcgccggag gcgtaatatt aattatccag ctggtgtctg cgggtcagag
     5701 agtcagtgcg ctgcgagcta ttggtgccag tgcgccgata ttgccaaagt tatttattct
     5761 gatttttctg actacccttt tagtacagat tggcatcatg ctagtggtcg ttccgggaat
     5821 tatcatggcc attttactgg ccctggcacc ggtgatgctg gtacaggaca aaatgggcat
     5881 ttttgcctcg atgcgtagca gtatgcggct gacttgggcg aatatgcgtc tggtggcacc
     5941 cgcagtactg agctggttgc tggcaaaaac actgttgctg ctttttgcct cttcttttgc
     6001 cgcattaacc ccggaaattg gtgccgtact ggcgaacacc ttgagcaacc tgatttcagc
     6061 cgtattgctc atctatctgt tccgtctgta tatgttgatt cgccaataac cttaagcatt
     6121 tgattacgga atcgtaaaat gaagcagttt cttgattttt taccgctggt tgtctttttc
     6181 gcgttttaca agatttatga catctatgcg gctactgcgg cgctgatcgt cgccacggcg
     6241 attgtgctta tctatagctg ggttcgcttt cgtaaggttg agaagatggc cctgatcact
     6301 tttgttctgg tggtcgtctt cggtggcttg acgctgttct tccacaatga tgagtttatt
     6361 aaatggaagg ttacagtcat ttatgctctg tttgcgggag ccctgttagt cagccaatgg
     6421 gtgatgaaaa agccgctaat tcagcggatg ctgggtaaag aactcacgct gccgcaaccg
     6481 gtatggtcga agctgaatct ggcctgggct gttttcttta tcctttgcgg tctggcaaac
     6541 atctacatcg cattctggct gccgcaaaat atttgggtca actttaaagt ctttggactg
     6601 accgccctta ccttaatctt cacattgtta agcggtatct atatctaccg ccacatgccg
     6661 caggaagata aatcctaacc agactgccag tccttcgggg ctggcgctgt atctcctctg
     6721 ttctcctcag gaaaatcata gtagcatcgc gcctgtgatt ttccttttaa gtcggtttta
     6781 ccatgtctac aacacataac gtccctcagg gcgatcttgt tttacgtact ttagccatgc
     6841 ccgccgatac caatgccaat ggtgacatct ttggtggttg gttaatgtca caaatggata
     6901 ttggcggcgc tattctggcg aaagaaattg cccacggtcg cgtagtgacc gtgcgggttg
     6961 aaggaatgac tttcttacgg ccggttgcgg tcggcgatgt ggtgtgctgc tatgcacgct
     7021 gtgtccagaa agggacgaca tcggtcagca ttaatattga agtgtgggtg aaaaaagtag
     7081 cgtctgaacc aatcgggcaa cgctataaag cgacagaagc attatttaag tatgtcgcgg
     7141 ttgatcctga aggaaaacct cgcgccttac ctgttgagta atagtcaaaa gcctccggtc
     7201 ggaggctttt gactttctgc ttactgaatt tcggtggtgc cgttaatttt aaacaggata
     7261 ttcaccacaa tcccactgcc tggcttaccc ggctcataac gccatctgcg catcgcattt
     7321 ttcacctcac gctcaaacat gttcgcaggc ttggctgaga ggatttgtac gttatccacg
     7381 cgaccatccg gcgtgacgtc aaatttaact ttaacctgcc cttcaatgcg caatgcctgt
     7441 gctcgtgccg gatactgcgg ctgattacgg cttaatgcgc gtggtcctga agccacactg
     7501 gtaaccggct tgctggttgc agccgttgct gtacttgatg tcaggcgtgc cggtgccgta
     7561 ttttcaaacg gtgatgccgg acgcgactct acgggtttga catcgcgttt tggctgctcc
     7621 tgtacctttt tcaccggctt cggttttggc ttaggtttcg gcttcggctt ttcaatgacc
     7681 accggtgctt cttttggcgg ttcggggatc ggctcaggtt ccggttctgg ctctaccacc
     7741 ggctccggtg gcggctgaac ggcttgtggc ggttcgagat cagcaggcgc aaccatcgtg
     7801 acagaaattg gctgcgcagg cgcaggtagt tcaataacct gatgtaccga ggtatagagc
     7861 agacccgcca caacagcacc atgaatgcag accgaaagta acgtcggcca ggggaagcgg
     7921 cgaggtaaat caagggtcat tgaggtcata atcatttcag tagaaaaacc aagcctcgat
     7981 tttaaatgca aatagcaatc atattcaata aggcaagacg atcaggatca gtgaaaatgt
     8041 catttttacc gtaattaacc ttacgttatg catggtctta acaaagacat catctttccg
     8101 ttgcagatct tcgccctttc aaataacgta ctttacaact ttcccgaaca aggagttgtg
     8161 cccgtgttgt atgttattta cgctcaagac aaagctgata gcctcgaaaa acgcctttcc
     8221 gttcgtccgg cacatttagc acgtttacag ttactgcatg atgaaggtcg tttgttgaca
     8281 gctggtccaa tgccagcggt agacagtaac gatccagggg ctgcgggatt tactggctca
     8341 accgttattg ctgaatttga atcgctggaa gcagctcagg cctgggccga tgctgacccg
     8401 tacgtagcgg caggagtcta tgaacacgtt tcagtgaaac catttaagaa agtgttctga
     8461 atagagattc aatatttaag ggcacggttt ttgcaaccgt gttcttgttt ggcctttcct
     8521 tatagccggt cagtgcattc accaccacaa gcagataata actttatctg tatttcttta
     8581 tatgatgtga gcatattcag gaaaaatgac ctttcctgag gtacagttgc tcgctcaaaa
     8641 cctttgatta ctgcggataa agtcatctac tattgtttaa cacattcacg cgctgcatgt
     8701 atgttcagaa aatgtgcacg ctaagataat taaacaaaat tgtggttaca gaggtatcag
     8761 tgagtcactg ggctacattc aaacaaacag caacaaattt atgggtgacc ctacggcacg
     8821 acattctcgc gctggccgtc tttttaaatg gattgcttat ttttaaaaca atctatggta
     8881 tgtcggtcaa tttgctagat attttccata tcaaagcatt ttcagagttg gatctctcct
     8941 tgctggcaaa cgccccacta tttatgctcg gcgtctttct tgtcctgaac tccattggct
     9001 tactgttccg ggcaaagctc gcatgggcaa tcagtatcat tttgttgttg atagcgctaa
     9061 tttataccct gcatttttat ccctggttga aatttagtat tggattttgc atttttacgc
     9121 tggtgttttt gctgatactg cgcaaagact tctcccacag tagcgccgca gccgggacaa
     9181 tttttgcatt tattagtttc acgacattac tgttttactc aacctacggt gcgctttatt
     9241 taagcgaagg ttttaatccg cgaatagaaa gtttgatgac cgcgttctat ttttcgatag
     9301 aaaccatgtc aaccgtcggc tatggcgata ttgtccctgt ttctgaatca gcacgattgt
     9361 tcactatttc ggtcattatt tccggtatta ccgtttttgc cacatctatg acctcaattt
     9421 ttggtccgct tatccgcggg ggattcaaca aacttgtaaa aggaaacaat catacaatgc
     9481 atcgtaaaga tcattttatt gtttgcggac attcgattct cgccatcaat acgattctgc
     9541 aactgaatca acgcggacaa aacgtaacgg ttatcagcaa cttgcctgaa gatgatatca
     9601 agcaacttga gcaacgctta ggcgataacg ctgatgttat ccccggtgac agtaatgaca
     9661 gttcagtatt aaagaaagcg ggaatcgatc gttgcctggc cattctggcg ctgagtgata
     9721 acgatgcaga taacgcgttt gttgtactct cggcaaaaga tatgagcagt gatgtcaaaa
     9781 cagttctcgc cgtcagtgat agcaaaaacc tgaataagat taagatggta catccggata
     9841 tcattctctc accgcaactg tttggcagcg aaattctggc gcgagtttta aatggtgaag
     9901 agattaataa tgatatgctc gtttcaatgt tgttgaactc cggtcatggc attttcagcg
     9961 ataacgatga acaagaaacg aaagctgaca gtaaagaatc ggcgcaaaaa tagagtttaa
    10021 agatggagga gtaaacttat tgggcaccgc aatggtgccc agtacattta gatattgtag
    10081 atcgcaaaca ataacgagtt acgctgtttg tttagaatgc acttcctgat gctgtggata
    10141 cgcatatttc ggcgcgattg cccctcaagc caacgcgatt tacgtcggct agcctgacgc
    10201 tgcatgcgcc agcgccccac ttccgttcta ctccgcttca tgttaactac tctatgcaat
    10261 aacagaatgg tcattatacc cccgcccccg acgctgacca gtgcttttcc cgcgttttta
    10321 tttgccagat gaatccatat gcgtaaactc ataacaatgc gctttcaaaa ggatttctaa
    10381 tctatgacaa ccgtttatac gttggtgagt tggttggcca ttctgggata ctggttgctc
    10441 attgcaggcg taactttacg cattctaatg aaacgacgcg cagttccctc cgcgatggcc
    10501 tggctgttga ttatttacat tctgccgtta gtcggaatta ttgcctatct tgccgttggc
    10561 gagctccatt taggcaaacg ccgcgctgag cgcgccagag cgatgtggcc ttccaccgca
    10621 aaatggctta acgaccttaa agcctgtaag catatcttcg ccgaagaaaa tagcagtgtc
    10681 gctgcgccat tattcaagct ttgcgagcgt cgtcagggga tcgctggggt caaagggaat
    10741 cagctacaac tgatgaccga gtcagatgat gtgatgcagg cgttaatccg cgacatccag
    10801 ctcgcgcgcc ataatattga gattgtgttt tatatctggc agcccggcgg catggcggac
    10861 caggtggctg aatcattaat ggccgctgcg cgacgcggca ttcattgccg attgatgctc
    10921 gactccgccg ggagtgtggc ttttttccgc agcccgtggc ccgagctaat gcgtaatgcc
    10981 ggtattgaag tggtcgaagc cttaaaggtc aatctgatgc gtgtgttttt acgccgtatg
    11041 gacctgcgcc aacatcgcaa gatgatcatg atcgataatt acatcgcgta caccggcagc
    11101 atgaatatgg tcgatcctcg ctacttcaaa caagatgcgg gcgtagggca atggattgat
    11161 ctgatggcgc gtatggaagg ccccatcgcc accgcgatgg ggattattta ttcctgcgac
    11221 tgggagattg aaaccggaaa acgtattctg ccgccaccac cagatgtcaa tattatgccg
    11281 tttgaacagg ccagcggtca caccattcac acaattgctt ctggccccgg ctttccggaa
    11341 gatctcattc accaggcatt attgactgcg gcttattcgg cgcgtgaata tttgatcatg
    11401 accacgccct actttgtgcc aagcgatgat ttacttcatg cgatttgcac ggcggcacag
    11461 cgcggggtgg atgtcagtat tatccttccg cgaaaaaatg actcgatgct ggtcggctgg
    11521 gccagtcgcg cattctttac ggaacttctt gctgctgggg ttaaaattta tcagtttgaa
    11581 ggcgggttac tgcataccaa gagcgtgctg gtcgatggcg aactaagtct ggttggcacc
    11641 gttaaccttg atatgcgtag tctgtggctg aatttcgaga ttaccctggc aatcgacgat
    11701 aaaggttttg gtgcagacct tgccgccgtt caggacgatt atatttcgcg ttcacgtctg
    11761 ctcgatgccc gtttatggct aaaacgtcca ttatggcaac gtgtcgccga gcgactgttt
    11821 tacttcttca gcccgttgct gtaaaacgtc gccaacagag gttaaacagg tagtgactat
    11881 ggatatggat ctaaacaatc gcctgactga agatgaaacg cttgagcagg cctacgatat
    11941 ttttctcgaa ctggctgccg acaatctcga cccagctgat gttctgctgt tcaatcttca
    12001 gtttgaagag cgcggcggcg cggagttatt cgatccggcg gaagactggc aggaacatgt
    12061 tgatttcgat ttgaacccgg acttttttgc cgaagtggtg attggtctgg cggacagtga
    12121 ggatggtgaa atcaacgatg ttttcgcccg cattctgtta tgccgcgaaa aagatcacaa
    12181 actttgccat atcatctggc gggaataaat aatcgctttc ggcgtgaata agggctgaca
    12241 actgtcagcc cttattgtgt ttaaagcgga tcgactttca ggcaagaaac ggcgtgtctg
    12301 aaactccctt ccagcacagg acgtgttttg gcgcactccg gaccggcaat cgggcaacgg
    12361 gtacggaaaa cacaaccgga aggcgggttg atcggcgacg gtaattcccc ttccagtaac
    12421 tggatggttt tgttcttctc cagatccgga tcaggtatgg ggactgccga catcaatgcc
    12481 ctggtgtaag gatgtagtgg attgtggtag acctcatcat aggtccccag ttctaccgca
    12541 tggccgagat acatcaccaa cacacgatcg gaaatgtgtt ttaccacggc caggtcatga
    12601 gcaataaaaa ttaatgacaa tcccatctca cgttgcagct gctggagcag gttgaccacc
    12661 tgcgcctgaa ttgacacgtc cagcgccgac accggctcat cacagataat cagcttcggt
    12721 tcaagaataa gagcacgagc aatcccgata cgctggcact gcccaccgga gaactcatgc
    12781 ggatagcggt taatcaggtt aggcaataac ccgactttca gcatcatcgc cttcacgcgc
    12841 tcgcgaactt cctgacgtga cattttcgga tgataagtac gcagtggttc agcgatgatc
    12901 tcgccgatgg tcatgcgcgg gtttagcgat gccaacggat cctggaaaat catctgaata
    12961 tcactgcgaa cggcacgcca ttcatcgggc ttaatgccca gcaactcttt acctaaccag
    13021 gcaacatgac cgtcggtcgc tttaaccagg ccaatgatgg cgcgagcaaa ggtggactta
    13081 ccgcatcccg attcccctac cacacctaat gtttcccctt catacaggcg aagcgtgaca
    13141 ccatcgacgg ctttgagcgt tttcggcggt tgccagaacc actgtttgcc atctttgatt
    13201 tcaaagtgca ctttcagatc ggcgatttca aggaggactt ttcttccttc ggttacagca
    13261 ttcataacag ttcctccacc ggtttaaagc aagcacacag atggccaggc gtaaactctt
    13321 ccagcggcgg tgcgctacta caaatttcca tcgcatgcgg acaacgtggc tggaacgggc
    13381 aacctttcgg taatcgcagc aggtttggcg gattacccgg gatggtcaac attgtttcac
    13441 cttccgcatc gagacgcggc accgcgttga gcaaaccgat agaataagga tgaacgggtt
    13501 gataaaagac atcgcgtgcg ttgccatatt ccatcgtgcg cccggcgtac attaccagca
    13561 ctttgtcaca gatccccgcc accacgccaa gatcgtgggt aatcataatg atggcggtat
    13621 taaattcccg tttcagttca tttaatagcg tcatgatctg cgcctgtacg gtgacgtcca
    13681 gcgctgtagt gggttcatcc gcaatcagca gcttaggtcg acatagcaat gccatcgcaa
    13741 tcataactcg ctgacgcatg ccaccagaaa actcgtgcgg gtacattttc atgcgtttac
    13801 gtgcttccgg catttttacc gcatcgagca tccgcaccga ctcttcaaac gcctcagctt
    13861 tgctcatgtt cttatgcagc atcagcactt ccatcaactg ctcaccgacg cgcatatagg
    13921 gattcaacga agtcattggg tcctgaaaaa tcattgagat ttgttcagcg cgcagtttat
    13981 tgagttcatg ctctggcaaa ttgaggattt cacgcccatt gaaggttgcc gatccgccaa
    14041 tacgtccgtt ggcagccaac aggcccatca acgcaaatgc agtttgcgat ttacccgaac
    14101 cagactcacc tacaataccc agcgtttctc cggcacgtag ggaaaaattc aaatcattga
    14161 ccgccgtgac gtcgccgtcc ggggtactaa aggtgacacg caaatctttc acgttcagca
    14221 gtgcgccagc ctgttgttgt gcgagcggca cagttgcagt ttcaattacg ctcatggctg
    14281 cactccttaa cgatctttcg ggtcgagggc atcacgcaag ccatcgccga taaagttgaa
    14341 acaaaacagc gtcaccacga ggaatcccgc tgggaacaac aataaccatg gagagacttc
    14401 catcgagttc gcgccatcac tcagcaatgc accccagctg cttaacggct cttgcgtacc
    14461 caaccccagg aagctaagga aagattcaaa gaggatcatg ctgggcacca gaagtgatgc
    14521 gtagaccacc accacaccga gtacattcgg cacaatgtgg ctaataacaa tgcccgatgt
    14581 cgatacaccg ccaacttgtg ccgcctcaat aaactctttg cgcttcagac tcagggtttg
    14641 cccacgcaca atacgagcca tatccagcca ggaaaccatg cctatcgcca cgaaaatcag
    14701 caggatgttt tgaccgaaaa aggtcaccag caaaatgacg aagaacatga atgggaagga
    14761 gttgaggatt tccagtagac gcatcattac cgaatccact ttaccgccca gataaccgga
    14821 aagcgaacca taaagtgtcc cgacgaccac agccaccagt gccgcagcaa caccgaccat
    14881 gagtgagata cgcccaccaa tcgcaacgcg cacaagcagg tcgcgaccgg atgagtcagt
    14941 accaaagtag tgaccggact ccatatccgg ggtgctggac atcatcgccc agtcagtatc
    15001 gtcataggca aactgggaaa gcatcggtgc caggattaca aataacgcga tcagcaccag
    15061 caccatcaga ctggcaaccg ccgcacggtt atgaataaaa cgtcgacgtg cgtcctgcca
    15121 caagctgcgc ccttcgacct ccagcttttc actgaaattt tccagcgtct cgctgttttt
    15181 cttacttaac atcatcgcga gctccagtat cagtaacgga ttttcgggtc gataaccgca
    15241 tatagcacat cgacaatggc attaaacaaa atggttaaag caccaaccag gatggtcagg
    15301 cttaacacta aggaatagtc acggttcaat gcgccattaa cgaacagttg tccaatcccc
    15361 ggcaaaccat aaatggtttc gataaccata gaaccggtaa taatgccgac aaatgcgggg
    15421 cccatataag agagcacggg taacagagca ggttttaatg cgtgacgtaa aatgatccgc
    15481 cgtataggta accctttcgc ccgggcagta cgaataaaat tggagtgtaa tacttcaatc
    15541 atagagccac gggtaatacg cgcaatactg gcgatataag cgagtgacaa cgccaccatc
    15601 ggcaatatca tgaatttaag cgccccacca ttccagccac cgcccggcag ccaatgcaaa
    15661 atgatcgcaa atatcatgac taataatggt gcaaccacaa aactggggat aacaaccccg
    15721 gtcattgcca gccccatcac ggtatagtcc catttggtgt tttgttttaa tgcggcaata
    15781 acgccagcac taacacccag tattaccgca aggaaaaatg ctgcggctcc cagtttggca
    15841 gaaacgggaa aactggatgc aaccaagtca ttgaccgaat aatctttata tttaaacgat
    15901 ggaccgaaat caccgtgcgc cagttgtttc aggtagctga aatactgtgt catgatcgga
    15961 tcattaagat gatatttcgc ttcgatattg gccatcactt ctggcggtaa agtacgttcg
    16021 ccggtaaaag ggcttcccgg cgcgaggcgc atcataaaga acgaaatagt aataagaata
    16081 aatagcgtcg gaatcgcttc cagacagcga cgtagaataa attttaacat tgccctacct
    16141 tctggcctgt gccttctgta atgcgataaa aatcagacac cgtggagcag gacactcctg
    16201 ccccacgtat tgccattagt gcttcacaat gtacatattc cgggtatagg tattatccag
    16261 cggatctttg ccggtatagc caccaaccca cggtttcacc agacgcgcat tcacgtagta
    16321 ataaacagga acaatggccg aatccttatc cagctgttgt tctgctttag tgtacagagc
    16381 tgtgcgctgc gcctcgtcag tcactttcag cgtttccgcc ataatgctgt caaaggccgg
    16441 gctcttataa tgcgcggtat tcatcgagct gttcgaaagc atggtgttca ggaaggaagt
    16501 tggttcgttg tagtcagcac accagcctgc acgggccaca tcaaaagtac cctggtgacg
    16561 ggtgtcgagg aacgttttcc actcctggtt aaccagtttg acgtttacac caatgttttt
    16621 cttccacaat gaagaggcag caatcgccag ctttttatgc agatcggagg tgttatacaa
    16681 caggttgatg gtcaacggtt tgtctgcggt ataacccgct tcagccagca gttttttcgc
    16741 ttcttcgtta cgtttttcct ggctccagcc aaaccattcc ggctgagtca attttgcgcc
    16801 atcagtatac ggtggagtgt aaccataggc gggcatgtcg ccctgcgctt tcactttatt
    16861 aacaatgata tcgcggtcca tacctagttt cagtgcggta cgcacacgca catcgttgaa
    16921 tggcggtttc tggttgttaa tttcgtaata gtaagtgcac aggtatggat caacgtgaac
    16981 ttcgtccggg atctcttttt tcagcttctg gaacaattcg atcggcatgt tgttataagt
    17041 catgtcgatt tcaccactac ggtagcggtt gacatcggta acttcagaag caataggcaa
    17101 ataggttacc tggttaataa cggttttcgc gttgttccag taggtcgggc tgcgttcaag
    17161 aacgattcgt tcgtttacga cccaatcttt taaggtatag gcaccgttgg tgacgatatt
    17221 accaggctgg gtccattttt cgccgaattt ctcgatagcg gcttttggca ccggtgaagt
    17281 tgatgggtga acaagtaatt tatagaagta cggaacgggt tcacttaagg tgacttctaa
    17341 tgtgtgatca tcaatagctt tcacgccgag atcggtaatc ggttttttcc cttcaagaat
    17401 ttcatcaata ccggcgatat gcccatattg cagataactg gcatacggag aagcagtgtt
    17461 cggatcaaca gaacgttgcc agctatacac aaagtcttgt gctgtgactg gcgtaccatc
    17521 agaccatttc gcatctttac gcaaatggaa ggtccagact ttcgcgtctt tattatccca
    17581 ggattcagcg acgccaggtg ccggatgacc gtcaagatcg ctgaccagta agccttcaaa
    17641 caggtctcgg ctgatattag actccggaac accttcaatt ttgtgcggat ctaatgactg
    17701 aacttctgaa ccattgttac gtaccagcgt ttgtttttcc gccagtgtga cgcctgcggg
    17761 tacatcagct gccagcgcga cattccctgc cattagcgca gccagaacgc cagctgctac
    17821 taaacttctc ttggtgatgt tggtcattgt tttttggact ccctcattat aattactggt
    17881 attaaccagc atgtgtaatc ccggattggg accctgcaca ggctcggaga ctttctgctg
    17941 tcaggttact ttatcgttac ggattgctat caccgacttt atttattctg gtgattcaga
    18001 cgatcacctt atgtcgattc gtactgtgtc tcccctgtcg agacacatta ttctttcgcg
    18061 acgctcttta attgttaatt attctcatta ccaataaaga atcgtctggc ggttgcccgg
    18121 aaagtaccaa atgcggttct tactcgccaa tacattttgc aaaattgtta agctattctc
    18181 ttttattgac cgtatcagaa tctatagctt gcatccatgc cttaacaaac atctttcaat
    18241 tcaatcaaat agagattggc taagcatgtc tatcttttct gattacagtt tcagtattga
    18301 aatgcacaat aatttaacaa ttgattatta tttagcactt tcctctacca aaggcagtgg
    18361 aattactaat atcatttcaa ttattcttca gcaagcccag gattatgatg tggcgaaaat
    18421 aacatgatgg tgaggagaag cagttaagaa aatccttata actaactgta atttgagaag
    18481 aatattttta cctattcctt ttgcttttga taactgcg
//
LOCUS       DAJTNA010000075        18127 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTNA010000075 DAJTNA010000000
VERSION     DAJTNA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18127
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.076"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(323..679)
                     /locus_tag="OO161_002143"
     CDS             complement(323..679)
                     /locus_tag="OO161_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5463205.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(746..1040)
                     /gene="yebG"
                     /locus_tag="OO161_002144"
                     /pseudo
     CDS             complement(746..1040)
                     /gene="yebG"
                     /locus_tag="OO161_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            1173..2351
                     /gene="purT"
                     /locus_tag="OO161_002145"
     CDS             1173..2351
                     /gene="purT"
                     /locus_tag="OO161_002145"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5463206.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(2407..3048)
                     /gene="kdgA"
                     /locus_tag="OO161_002146"
                     /gene_synonym="eda"
     CDS             complement(2407..3048)
                     /gene="kdgA"
                     /locus_tag="OO161_002146"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5463207.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGLKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(3085..4896)
                     /gene="edd"
                     /locus_tag="OO161_002147"
     CDS             complement(3085..4896)
                     /gene="edd"
                     /locus_tag="OO161_002147"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5463208.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(5131..6606)
                     /gene="zwf"
                     /locus_tag="OO161_002148"
     CDS             complement(5131..6606)
                     /gene="zwf"
                     /locus_tag="OO161_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5463209.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            6944..7813
                     /gene="hexR"
                     /locus_tag="OO161_002149"
                     /gene_synonym="yebK"
     CDS             6944..7813
                     /gene="hexR"
                     /locus_tag="OO161_002149"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5463210.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            7941..9383
                     /gene="pyk"
                     /locus_tag="OO161_002150"
     CDS             7941..9383
                     /gene="pyk"
                     /locus_tag="OO161_002150"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5463211.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(9443..10285)
                     /locus_tag="OO161_002151"
     CDS             complement(9443..10285)
                     /locus_tag="OO161_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR5463212.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHETTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIEP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
     gene            complement(10308..11133)
                     /locus_tag="OO161_002152"
                     /pseudo
     CDS             complement(10308..11133)
                     /locus_tag="OO161_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(11226..12023)
                     /locus_tag="OO161_002153"
     CDS             complement(11226..12023)
                     /locus_tag="OO161_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR5463213.1"
                     /translation="MIIDAHTHIGDFVKISMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQDDNNERMLRLVRQHAKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSEKVQDYIRLAQKYDLPVVSHTSNDYESSPQLFYEMALKY
                     PDVNFVMCHMGLATDNQEAIELIAKLPNLYGDTVWVRADMVYQAIKICGSDKILFGTD
                     NPINGLDTYNDDDFYNKYFTELKSKLTQSEFEQLMFTNAKKNFKIKI"
     gene            complement(12045..13844)
                     /locus_tag="OO161_002154"
                     /pseudo
     CDS             complement(12045..13844)
                     /locus_tag="OO161_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            complement(13862..14695)
                     /locus_tag="OO161_002155"
     CDS             complement(13862..14695)
                     /locus_tag="OO161_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5463214.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFHG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEADKIDGAGHLTIFIQIILPLAKPILVFIGLVSFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
     gene            complement(14698..15986)
                     /locus_tag="OO161_002156"
                     /pseudo
     CDS             complement(14698..15986)
                     /locus_tag="OO161_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(15989..17220)
                     /locus_tag="OO161_002157"
                     /pseudo
     CDS             complement(15989..17220)
                     /locus_tag="OO161_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
     gene            17492..>18127
                     /locus_tag="OO161_002158"
     CDS             17492..>18127
                     /locus_tag="OO161_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5463215.1"
                     /translation="MVLIKKNVLSASIATVILGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGA"
BASE COUNT         5108 a         4253 c         3877 g         4889 t
ORIGIN      
        1 gctcctcaat aaccattttt agtgcctgta gcatagcgga tgaggatgtc agaaaatgta
       61 tatcgagaca aaagatgcgc aaataagcgg ggcggtaggc cgttattcaa agaaattcgc
      121 gccaggtaag gtatttactg tatatcaggt gataagggta ttttaggtga aagtagtttg
      181 tcgttttgag tggcattgtt tgatgctgat tgcccgatgc gacgctgttg cgtcttctca
      241 ggcctacgat tgtggtgcgt tctggggcgg acaaggcact cgcgccgcat ccgccaacgg
      301 tcaatcgaat tatcagaagc cattaacgcc gctgatattc cgccattccc gctttgcagt
      361 ccacgctgac ctggtaatga atatcggcac ttttaccacg cacggttagc ggtactgacc
      421 atttatcatc tttaccctga atgtcctgca aactgaccca agccacggga tcggcctgac
      481 cgacaatttt ttgatcatct gcccaacgcg ccacgcgatt ttgttgataa tcacgtttta
      541 cgctcgcggc aattccggca gcatccagac cttcacactt tgggaaagtg accgacttgc
      601 tggtttcatt attggcagcg aaaactgatg cgcaggcaga aaccaacaac agccctaaaa
      661 acgcccctct ttttttcatg tttttctcca tagcacaatg attcaggaga aagcatggta
      721 caaattgtca ggagcgcaag ttgcttcagg ccgcgtgtga ggcatcttcc tcttcggatt
      781 cagcaccgac gacctgtggg tggggacggt aatttgccgg ttttcagaat ggtgctcagc
      841 acatcttttt gttctgccag ccatagcgaa agggcttcac gttgctcgtc ttccatttgc
      901 actggagaat tagtcagcca ggtgtcaagc agatccgccg tatcaagcat tttgtcataa
      961 gcatcggctt cctttttgct ggtaaacgac attttctctt cgccctcacg aatgactacg
     1021 tatttaactt caaccgccat ttgcagcctc tcataataac tgtgatttta tacagtatat
     1081 ttcttttcgg ttgagaatca acatcagcaa taaagacaca cgcaaacgtt ttcgtttata
     1141 ctgcgcgcgg aattaatcag gggatattcg ttatgacgtt attaggcact gcgctgcgtc
     1201 cggcagcaac tcgcgtgatg ttattaggct ccggtgaact gggtaaagaa gtggcaatcg
     1261 agtgtcagcg tctcggcgta gaggtgattg ccgtcgatcg ctatgccgac gcaccagcca
     1321 tgcatgtcgc gcatcgctcc catgtcatta atatgcttga tggtgatgca ttacgccgtg
     1381 tggttgaact ggaaaaacca cattatatcg tgccggagat cgaagctatt gccaccgata
     1441 tgctgatcca acttgaagag gaaggactga atgttgtccc ctgcgctcgc gcaacgaaat
     1501 taacgatgaa tcgcgagggt atccgtcgcc tggcggcaga agagctgcag ctgcccactt
     1561 ccacttatcg ttttgccgat agcgaaagcc ttttccgcga ggctgttgct gacattggct
     1621 atccctgcat tgtaaaaccg gtgatgagct cttccggcaa ggggcagacg tttattcgtt
     1681 ctgcagagca acttgctcag gcatggaagt acgctcagca aggcggtcgc gccggagcgg
     1741 gccgcgtaat tgttgaaggc gtcgttaagt ttgacttcga aattaccttg ctaaccgtca
     1801 gcgcggtgga tggcgtccat ttctgtgcac cggtaggtca tcgccaggaa gatggcgact
     1861 accgtgaatc ctggcaacca cagcaaatga gcccgcttgc ccttgaacgt gcgcaggaga
     1921 ttgcccgtaa agtggtgctg gcactgggcg gttatggtct gtttggtgtc gagctgtttg
     1981 tctgtagtga tgaggtgatt ttcagtgagg tctcccctcg tccacatgat accgggatgg
     2041 tgacgttaat ttctcaagat ctttcagagt ttgccctgca tgtacgtgcc ttcctcggac
     2101 ttccggttgg cgggatccgt cagtatggtc ctgcagcttc tgccgttatt ctgccacaac
     2161 tgaccagtca gaatgtcacg tttgataatg tgcagaatgc cgtaggcgca gatttgcaga
     2221 ttcgtttatt tggtaagccg gaaattgatg gcagccgtcg tctgggtgtt gcgctggcga
     2281 ccgctgagag tgttgttgac gccattgaac gcgcgaagca cgccgccgga caggtaaaag
     2341 tacagggtta aacccggcaa aaaaacgcta caaaaatgcc cgatcgtaga tcgggcgttt
     2401 tgacttttac agcttagcgc cttctacagc ttcacgcgcc agcttagtaa tgcggtcgta
     2461 atcgcccgct tccagcgcat ctgccggaac cagccaggaa ccaccgatgc acagcacgct
     2521 tttcagcgcc aggtagtcac ggtagttagc cggagaaata ccacccgtcg ggcagaaacg
     2581 gacctgggag aacggacccg cgatcgcctg cagggctttc aagccgccgt tagcttcagc
     2641 cgggaagaat ttgaactctt tcaaaccgta gtccataccc agcatcagtt cggaaacagt
     2701 gctgatcccc ggaattagag gaatagtccc ttcggtagca gctttcagca gcggctcggt
     2761 cagacccggg ctaattgcga actgtgcacc cgcttcagtg acttctgcca gctgctgtgg
     2821 attcagcacc gtaccggcac ccacaatcgc ttcaggcact tctttggcga tagcacggat
     2881 agcgtcaact gcacactcgg tacgcagagt cacttccaga acgcgcaccc caccagcaac
     2941 caacgctttt gccatcggca ctgcgtgttc cagttttttt accacgataa ccggtacaac
     3001 cgggccggtg gtcaggattg attctgcact tgttttccag tttttcatca gagttttctc
     3061 tcgcctgatt acaaatttgt cgtcttaaaa agtgatacag gttgcgccct gttcggcacc
     3121 ggacagtttt tcacgcaagg cgctgaataa ttcacgtcct gtccccacgc gtgacgcgct
     3181 caggtcagga atgtgcggtt cgcgagcagc cagttccgct tcgtctacca gcagcgtcag
     3241 ttcgcctgtc tgtccattca cacgaatgat gtccccgtcg cgcacttttg ccagcagccc
     3301 gccatcgtag gcttctggtg ttacgtggat agctgacggc actttacctg aagcgccgga
     3361 gagtcgtcca tcggtaacta acgcaatttt gaaacaccgg tccaataata caccaagtgg
     3421 cggcatgagt ttatgtaatt ctggcattcc gttcgctttt ggcccctgat gacggacaac
     3481 aacgacacaa tcgcggtcca gcaaacccgc ttcaaaggcc ggcataacgt catgctggct
     3541 ttcaaaaaca accgctggcg cttcaatcac ctggttctca accggcacgg cagaggtttt
     3601 cataaccgca cggcccaggt taccgcttaa cacttttgtc ccaccatgat gagagaaagg
     3661 ttgttcgaag gaagcgatca cattgctgtc gagtgatttt tccgcccctt cccgccagtc
     3721 cagttcacca ttattcagcc atggttcaag ggtataacga gacagaccaa agcctgccac
     3781 cgtattgaca tcttcatgca gcaggcctgc tttgagcagt tcacgcacca gaaccggtac
     3841 gccacctgcc gcctggaagt ggttaatatc ggccggaccg ttcgggtaga gacgtgccat
     3901 cagcggtaca acatcagaaa ggtcagagaa gtcatcccag ttaatctgaa taccggccgc
     3961 gcgcgccatc gccaccaggt gcatggtgtg gttagtggaa ccaccggtcg ccagcagtgc
     4021 aacgataccg ttcaccacca ctttctcatc gatcatctta ccgatcggca tccattcatt
     4081 accattaccg gtcatgcgtg taacctgacg cgcagcggca gcggtcaaag catcgcgcag
     4141 cggagaatcc ggatgaacaa aagaagagcc tggcgactgc atccccataa attccaccac
     4201 catctggttg gtgttggcag taccgtagaa agtacatgtt cccggcgcat ggtaagacgc
     4261 ggcttctgac tccagtaagg ccatgcggtc cactttacct tcggcataaa gctggcgaat
     4321 acgcactttt tctttatttg gcaaaccgct tgccatcggt ccagacggca caaacaccgc
     4381 aggcaaatga ccaaacgaca gggctgccat cgtcagaccc gggacaatct tgtcgcacac
     4441 accgaggaac agagcaccat caaacatgtt atgggacagc cccaccgccg cagacatcgc
     4501 tatcacttcg cggcttagca gcgacaattc cattccatcc tgcccctggg tgacaccatc
     4561 acacatcgcc ggaacaccgc ccgcaacctg accaaccgca ttcgcttcat gcagggcttt
     4621 acgaatgatt tctggatagt gttcataagg ctggtgcgcg gagagcatgt cgttatagga
     4681 ggtgatgatg gcgatattgt tacgcaacat gcttttcaaa gaggctttgt cttctggctg
     4741 gcaggcagcg aaaccgtgtg ccaggttacc gcatgccaac tgcgaacgat gaacggtcga
     4801 cgttttcgct tgttctatcc gggcgagata agcagagcga gtctcgcgcg aacgttcaat
     4861 gattcgattt gttacgcgta acaattgtgg attcataaag gctcctgaaa ttgagttgtc
     4921 agagcaggat gattcacaac gcgtttcatt cagaggattt atgagtgaaa cgcctgtaac
     4981 cggagctcat agggcaaaaa cgtttcagtc agtgtaataa aaaaagcctc gtgggtgaat
     5041 ccgcacgagg cctgaaagtg taaaaattgt tctacaatct gtgcaagatc atgttaccgg
     5101 taaaatgacc ataaaggata agcgcagata ttactcaaac tcattccagg aacgaccatc
     5161 acgggtaatc atcgccaccg aggcaacggg tccccaggtt ccggcctgat acggtttcgg
     5221 cgcatcattg tccatcgccc acgcctcagt aatggagtct acccatttcc aggcttcttc
     5281 cacttcatca cgacgtacaa acagtgcctg aataccacgc atggtttcca gcagcagacg
     5341 ttcataggca tccgccagat gcgtctgatt aaaggtttct gaatagctca gatccagctt
     5401 ggtgatttgc aggttatgtt tgtggtcaag gccaggaact ttattcagta cctggatatc
     5461 cacgccttca tcaggttgca gacggatagt cagtttattc tgcggcagat cctgccacga
     5521 ctctttaaac agattcagtt caggtgtttt gaaatagacc acgacttcag aacatttggt
     5581 cggcagacgt ttaccagtac gcaggtagaa tggcacaccg gcccagcgcc agttatcaat
     5641 gtcgacgcgg atcgccacga aggtttctgt attgctgctc ttgttcgcgc cctcttcttc
     5701 cagatatccc ggcacttttt tgccctgggc gaagcccgca gtatattgcc cacgtacggt
     5761 tttttcgcgt acgttggagc ggtcgatgcg gcgcagggac ttcagtactt tcactttttc
     5821 atcgcggatg ctgtctgcgc tcaggtcaga tggcggagac atcgcaatca tgcagagaat
     5881 ttgtagcagg tggttctgaa tcatgtcacg catctgaccg gctttatcaa aatagcccca
     5941 gcgcccttcg atccccactt cttctgccac ggtaatctca acatgatcaa tggtgcgatt
     6001 gtcccagtta ttcacaaaca gggagttagc aaaacgcagc gccaacaggt tcagcaccgt
     6061 ttctttacca agatagtggt cgatacggta aacctggcac tcctcgaagt attcgccaac
     6121 ctgatcattg atttcctgcg aggtcgccag cgacgtcccc agcggtttct ccatgactac
     6181 gcgtgccggt ttagcattca gttttgcctc gccaagccct ttgcaaattg cgccaaaagt
     6241 gctgggcggc atggcaaagt agttaatggt gatacgattt ttttgatcca gcatcgcgcc
     6301 gagacggttg aatgcagcag tgtcattgac atcgagatta caaaaatcca gacgtgcact
     6361 cagggtgtcc cataaacctt catcaatggt ttctttcatg aaagtttcga gcgcctcgcg
     6421 gacaactttg gtatatgccg ctttatccca gtcagcacgc cctacgccga taatccgggt
     6481 gtccgggttg agctgaccgg ctttttccag ttgatacagg gaaggcagca atttacgacg
     6541 cgcaaggtcg cctttcgcgc cgaaaatgac caagtcacag gcctgggctg tttgcgttac
     6601 cgccatgtca ttctccttaa gttaactaac ccggtactta agccagggta tacttgtaat
     6661 tttcttacgg tgcactgtac tgcttttacg agcttgcgaa aactgtaaac gcttatccac
     6721 ccgtgcgatt acgggaaaag cgcgcaaagt gcggcaaaac tgataaaaaa atcatcgttt
     6781 tccattcggt aaaaatctga cactgatcat gttatgaaaa aaaataacaa cttttttatc
     6841 tgcttttgtc attaacacgg cacacaggcg taatatctga caaaactgca tttcgatttc
     6901 tttcagtgcg gaaatcgtca ttacccgtga gtctctttac atcatgaata tgctggaaaa
     6961 aatccagtct cagctggaac atttgagcaa atcagagcgc aaagttgccg aggtcattct
     7021 ggcttcgccc gataacgcga tccattcgag tattgctgct atggcactgg aagccaatgt
     7081 tagcgaaccg acggtgaatc gtttctgtcg cagcatggac acgcgcggtt ttcctgattt
     7141 taaacttcat ctggcacaga gtctggcgaa tggcactccc tatgttaatc gcaatgtcaa
     7201 tgaagatgac agcgttgaat catacacagg gaaaatattt gagtccgcaa tggcaacgct
     7261 tgatcatgtc cgtcattcac tggataaatc tgccatcaac cgcgccgtcg acttgctcac
     7321 tcaggcaaaa aaaatcgcct ttttcggatt aggctcttca gccgccgttg cccacgatgc
     7381 gatgaataag ttctttcgtt ttaatgttcc ggtggtgtac tccgatgata tcgtgctgca
     7441 acgcatgagt tgtatgaatt gtagcgacgg agacgtggtg gtgctgattt ctcacactgg
     7501 aagaacaaaa aatctggtcg agctggcgca gctggctcgc gaaaacgacg caatggtgat
     7561 tgccctcacc tctgcgggta ccccgctcgc ccgggaagca acgctggcaa ttaccctcga
     7621 cgtaccggaa gatactgaca tttatatgcc catggtttct cgacttgcac agctgaccgt
     7681 gatagatgtg ctggcgacag gatttacttt gcgacgcggt gcaaaattca gagataactt
     7741 gaagcgggtc aaggaagcgc tgaaggaatc gcgttttgat aagcagttac ttaatttaag
     7801 tgacgatcgc taaaaacgac tgtcactgtc ctaatcttat acgacatccg aatgagatta
     7861 atttatcgcc atcgcggcgt tatttcattc ggatttcatg ttcaagcaac acctggttgt
     7921 ttcagtcaac ggagtattac atgtccagaa ggcttcgcag aacaaaaatc gttaccacgt
     7981 taggcccagc aacagatcgc gataataatc ttgaaaaagt tatcgcggcg ggtgccaacg
     8041 ttgtacgtat gaacttttct catggctcgc ctgaagatca caaaatgcgc gcggataaag
     8101 ttcgtgagat tgccgcaaaa ctggggcgtc atgtggctat tctgggtgac ctccagggac
     8161 ccaaaatccg tgtatccacc tttaaagaag gcaaagtttt cctcaatatt ggggataaat
     8221 tcctgctcga cgccaacctg ggtaaaggtg aaggcgacaa agaaaaagtc ggtatcgact
     8281 acaaaggcct gcctgctgac gtcgtgcctg gtgacatcct gctgctggac gatggtcgcg
     8341 tccagttaaa agtactggaa gttcagggca tgaaagtgtt caccgaagtc accgtcggtg
     8401 gtcccctctc caacaataaa ggtatcaaca aacttggcgg cggtttgtcg gctgaagcgc
     8461 tgaccgaaaa agacaaagca gacattaaga ctgcggcgtt gattggcgta gattacctgg
     8521 ctgtctcctt cccacgctgt ggcgaagatc tgaactatgc ccgtcgcctg gcacgcgatg
     8581 caggatgtga tgcgaaaatt gttgccaagg ttgaacgtgc ggaagccgtt tgcagccagg
     8641 atgcaatgga tgacatcatc ctcgcctctg acgtggtaat ggttgcacgt ggcgacctcg
     8701 gtgtggaaat tggcgacccg gaactggtcg gcattcagaa agcgttgatc cgtcgtgcgc
     8761 gtcagctaaa ccgagcggta atcacggcga cccagatgat ggagtcaatg attactaacc
     8821 cgatgccgac gcgtgcagaa gtcatggacg tagcaaacgc cgttctggat ggtactgacg
     8881 ctgtgatgct gtctgcagaa actgccgcag ggcagtatcc gtcagaaact gttgcagcca
     8941 tggcgcgcgt ttgcctgggt gcggaaaaaa tcccgagcat caacgtttct aaacaccgtc
     9001 tggacgttca gttcgacaat gtggaagaag ctattgccat gtcagcaatg tacgcagcta
     9061 accacctgaa aggcgttacg gcgattatta ccatgaccga atcgggtcgt accgcgctga
     9121 tgacctcccg tatcagctct ggtctaccaa ttttcgccat gtcgcgccat gaacgtacgc
     9181 tgaacctgac tgctctctat cgtggcgtta cgccggtgca ctttgatagc gctaatgacg
     9241 gcgtagcagc tgccagcgaa gcggttaatc tgctgcgcga taaaggttac ttgatgtctg
     9301 gtgacctggt gattgtcacc cagggcgacg tgatgagtac cgtgggttct actaatacca
     9361 cgcgtatttt aacggtagag taatctcacc tgccccggcc gctgatggcc ggggtattta
     9421 gcaacacata aattaggact acttattcaa tccaatcata atcacagccc cctgtaaaat
     9481 gaatacaggg atagcctgaa tggagatcta aatagcttgc aagacgtcgc atacccgctt
     9541 cctctgcaac actatgtcca ataactgcca tcggtaagcc actatctttg gagtattgtg
     9601 tctgcaccca tgttttaatt gaatcgttaa tcgtaatata ataatcagcc tgatattcca
     9661 tatattgtat tggatcacaa aaacagcctg ccccaatgcc aacagttctg actactctgg
     9721 ctttatctcc ataaaattga atgccaggaa gattatatat tttcaaattt ttggcaaaac
     9781 gtttggtaat gttaatggca ctgtcaggtt ctatagcata tacacagtac atctcatcag
     9841 aagcaacaat atttgtaaca tccagttcta actgctgagc caaagcctta gacattccga
     9901 acgtaggctc tcgatccatg acatcatggc aacgaatgat taccaaatta tttttatgca
     9961 aaaactcctt tttttgctca attattttta aataactgag ttcaccgcga gtataatcca
    10021 ttatttttct ggcccatgaa aactcaagat cgtcaccctc cagatcagca taagaataaa
    10081 atgtagtctc atgtgtaata atgatattga caccttcaaa aacagccttt cttaaaacat
    10141 ccattgaggc aatccagcag gtcccaattt tattaataag tgtcgcctca gcaccaaaaa
    10201 taattttatc aacagttggt tgcgaaacag caaccattga atttaaaaaa caattaagat
    10261 ccgcggctgt tttaatatga tgcattcaac atccttcaca ggtttcatca gcaataaaat
    10321 aatttcaaca tttctgggaa acgttttgcc catgcagact cagagtgggt atcacctccc
    10381 cataaatgat aatctatagt tacattgcgt tgtttacgga gactcacgca taatttttca
    10441 gcaccggaaa tatagacttc aggaaagtct tcgcgcatac cactggtttc ctgcgtaccg
    10501 acatcgagat atacagtaag atcttcaacg atgttgtttt cctgaataaa attcagcatt
    10561 gcgctgtcat tgaaccatag tgccggcgaa aaaacgccaa cttttgaaaa aacgtcctga
    10621 cgaagaatta cggcaaacaa actgatgtac gcacccatag aactacctgc tatcgctgta
    10681 ttattacgat ctgggagagt tctacaatta ctgtcaacat agttcttgag cgtattcaca
    10741 ataaacgcct tttcctccac tataatccac agcagggtcc cagccaggta agggaaaaga
    10801 tgtgtttcgc ggccatggag aatactcatc catacggctg aatttaccat gagacgatgc
    10861 gttatcaatc cccacaatga taacaccgtc gtattgtgaa tttgggaaaa aagcatctac
    10921 cgcctcaggt atatcccata catgtggacc aaaagataca gatttatcaa ataaattttg
    10981 cccatcgtgc atatatatta cagggtaact gatatcagat tcataataga acgcaggcag
    11041 taatattctt atttcgcgac tactcgccaa ttgtggcata ggaaaagatt gcaacacatg
    11101 atagcgattc ccatagttct caatatcgtt catgatttaa attaacctca atgcaaaatg
    11161 aaatataaca tctaagcact cccctaccac attctatatg gggagcactt agaaatgact
    11221 aataactata tttttatctt aaaatttttt ttcgcattgg taaacataag ttgttcaaac
    11281 tcactttgcg ttaatttact ttttaattca gtaaaatact tattataaaa atcatcatca
    11341 ttgtacgtat ccagcccatt aataggatta tcggtaccga ataaaatctt atcactacca
    11401 cagattttta tcgcctggta aaccatatcc gccctaaccc acacagtatc gccatagaga
    11461 ttaggaagtt ttgcaatcaa ttcgattgct tcctgattat ctgtcgcgag ccccatatga
    11521 cacataacaa aattcacgtc tggatactta agtgccatct cataaaataa ctgtgggctg
    11581 gattcataat cattagaagt atggctaact accgggagat catatttttg agcaaggcgt
    11641 atataatcct gaactttttc tgagttgaaa gccatttttg aatgataagg gtgaactttg
    11701 atgccataaa tgatatctcg attgctggca atcatatctt caaagtcttg atcacaactt
    11761 tccagacgag gcttaatcca cataaacgcg ccaattcgct ttgcatgttg acgaacaagt
    11821 ctgagcatac gttcattgtt atcatcctgt gtaacttgat cttcatccgg aatcgggttc
    11881 tggtcatggt caacttcaac agcagatcca caggaacata acgcaaaatc aatattatac
    11941 ttgtccagag atgccagaaa aacatcttct ggcatgctga ttttcacaaa atcaccaata
    12001 tgagtgtgtg catcaattat cataacaaac atcctaattg aaatttaaaa atcataaaga
    12061 tcagcagcag gtaaaccttc aatttcataa ctgaaaccgg agatctccat acttcttccg
    12121 tcataaataa agttggcagt atcttcacta ttatttaaga agaaagtgat gttatcgcgt
    12181 tgataagcga tcaccgtagg atgttcaaca tctttccatt gtattaatgc ctcacaccat
    12241 tgtggatgtt ttttacgtag acgtattaac caacgtataa attgccgtaa ttcaagatct
    12301 tgtttgtttt catcccatat catacatttt ctattattct caagcgtcat tgatttaaaa
    12361 ccatcaattc ctatttcgct accgtagtag atacaaggag agcccgcttg agttgacata
    12421 aataaatagg cgagtttaac ttttcgttta tcattcaaac aaacgcttaa aatacgcgcg
    12481 gtgtcatgac tatccagaag attaaacata acttcagtta tatttttagg ataacaaaga
    12541 taagaacgtg taacggatgt cataaattct tgttttgttg tatcctgtaa cgcaaaataa
    12601 tcaataatcc catatgttaa gggataattc attaatgaat caaactgatc accacgcaac
    12661 tagggggtgc cctcatgcca tatctcactc aaaatataac attcaggttt gatatcttta
    12721 atcacctttc tgaaatctcg ccagaaatga tgatccactt cattcgcaac atccagacgc
    12781 caaccatcaa tatcaaaatt ttgcgtccaa tagcgaacta tactaagtaa gtattcacgg
    12841 cactcttcat tctcagtatt tagcttaggc atttcaataa cattgccaaa ggtttcatag
    12901 ttaaagtttt taaaatccca ttcacttttt ggagtatcgg gataaacagg caatttatga
    12961 atccagaacc aatcggcgta acgtgaattg gcaccattgc gtacaacgtc aagccatatt
    13021 ggagagtcag cgcctaaatg attgaatact gcatccaaca tgatttttat tccacgctta
    13081 tgcgcttctt caatcaaagt atggaaagca atattcccac ctaaatgtgg atcaacacta
    13141 aaatgatcga tggtgtcata tttatggttg gatgctgagg taaaaatagg acaaaaataa
    13201 atcccattaa tacccagatc ttccagataa tcgagtttat caataacccc ccacagatcc
    13261 ccacccataa agttgaatga agtcggtgta ctgccccacg gttctacccc ttctggggat
    13321 atttcaggtc tgccgttaca gaaacggtct ggaaaaattt ggtaccatac cgtatttttt
    13381 acccaggagg gggtatttag aacgtcaatt ttattaagat aagggaagca aaaaaagtta
    13441 tttaagcggc ttaactctcg ttcttcgcac tccggtgaac tgatatcaac acaccttttc
    13501 tcaccaaaaa gaaacttctc tccctctttc ccaaaaagta taaaaccgta acggcaacgg
    13561 cgtttttgag gtttgaacac tgcaaaccag tgatcatgaa attcggtgac agcttcttgt
    13621 tccatctcga tctcgttgcc gccaatccag ccgaacgctt ctgtcccggc catgttaccg
    13681 ccgtcaagac caccttcagc ccaattatat ggatcgccta tccagagaat aactttatca
    13741 acttctcctc ttaatgtgcg taaccgtaca tgcaaagttt cgttatcata ggcatagcta
    13801 tcttcacttt ttgctgagtg ttttaacgaa ctaactgtaa tcataaatac taatccctaa
    13861 attattcctt aacggcaccg ctaacaagtc cagtagttat atgcttctgt gtgaatacaa
    13921 ataacaatgt gataggtact gcaactaaaa gcgccccagc agcaaaaagc gtataattct
    13981 ctgctgaatt agaagcaatc caactaaaaa tgccaatcgc cagggtcatt ttatcctcac
    14041 tacgaagcac caacgttggc aaaatgaagt ccatccatgg tccagtgaac gaaactaatc
    14101 caatgaaaac gagaatcggc ttagccaatg gcaatattat ctgaataaaa atagtcagat
    14161 gccctgctcc atctatttta tcggcctcat ccaaagaagt tggaatagca tcaaaataac
    14221 cttttaccaa ccacgtcatg aagggcaatg aaccggttac ataaaccaga agcaacccag
    14281 tatatgtgtc tatcaaattg gcctttgata gcaggatata aatggcagtc attgataaaa
    14341 atgcaggaaa catttgcaat acaagaatac tcattaacgt attacgcttt ccatgaaatc
    14401 gataccttga aaaaacgtag gcggtcatcg tcacaacgaa cagtgaaatt aacatgttag
    14461 cggtagcaag aatgaaagtg ttcaaatacc actgcatata tggcgtatcc gtgaacaacg
    14521 ttatgtagtg gtcaaatgta aactcgatgc cactaaatgt tgaactaaat agattattcc
    14581 cttttttaaa agatgccatt atcgtccata tcactggccc aagcacaaga atagagttgc
    14641 agattaaaaa aagataaact aagagcgttg atattttacg taaaaattta tccattgtca
    14701 catccctaca tcatctttaa atgatttcat ccgtctgaat tgccatattg ctataaccga
    14761 taagaagata aatattatga tggagattac agaagctata tgatattgct ggaaattcaa
    14821 tgtgagttta taaatccagg taataaaaat atcggtatgc ccagcgaatc tgtattccgg
    14881 attagtcgga ccaccttcag tcagaagata aattgcacca aaattattga aattatatgc
    14941 aaatgtcatt accagagttg acgcaacctg atgtaacacc attggcaatg taatttcctt
    15001 aaactgttgc catttagttg cgccatcgac ctcactagcc tcatacaaat ctcgggatat
    15061 gtttgttaat gcacccgcga tcaatagcat aaaatacggt gcccctaccc aaacacaaac
    15121 ggcaattact gttatttttg catttaatgg aaccgacaga aaatcaactg aattcattcc
    15181 gatactgttc agaaacgagt taaccggacc aattccattt aaaagaagac gcaaaataag
    15241 taacgttaca aatgtgggta tggcatatgg cagtataaat atcaatcgcc agacttttgc
    15301 gaaacgtata tttttttagc cagtgctaaa gccagaataa agccaaaact gaaggtaaaa
    15361 accgtcgcgc aaattgccca aataaatgtc cagagagcga cacctagaaa cgtatcagac
    15421 catattttaa atttaaacaa cattatgaaa tttttaatcc caacccaatc aaccaggttc
    15481 tttggtggga tgtgatttgg cgcagcatag tttgtaaacg atacaagaac tgtaattaca
    15541 ataggtaaaa caatgaatgc tatacttgct aaaaatgcgg gcgttaggac tataaatgca
    15601 aatttatcct catagagctt atataactgt tgtttaaaag tcagatggca ataagatgag
    15661 ttccgcgcat ctttaatatt aaggatgtaa atagtggcga ataagccaac aataatggtc
    15721 gctatcacgc cctctacaag cataaatata gaattgtcac cctgaataac agtcaaccct
    15781 tttcgaattt gcgcgacatc acctaatgtg aacaaaccta ttaatgtatg cacaatatcg
    15841 gaaagataaa gcaaaaagca tacttgtatt gcaaagaata tcccgccttt cacataataa
    15901 tgacgtgata actgagtccc gcccataatc aaacaggaaa taggtagcac aggtgcactc
    15961 ttcattttca gttgttgtgt attcacaatt actcctgaag cgaaagttgt tcttcaataa
    16021 ttgtttgtgc atgatttaaa acttcatccg gtgttttatt ctcaacccag gtagccaaaa
    16081 tagcattagt cattggagac catagataag acatttccgg tattgatggc attgcgtcag
    16141 aataattagc ttgagcaata attgctttta tagcttcact acctgtctgg ctaattttgt
    16201 tcataagtga atcgattggt gggatgagat tcgtcatttc ataacgcttc atcaacatct
    16261 tatccgttgt caggtagtca gcgaataatt cagcggcatg aggatatgcg gtaaatgagc
    16321 ttacaacggc cagccgtact gttgaaaatg tgcgaggctg atgaccatct aatgttggga
    16381 tgcgtgtcac gccaaaattg attccgctgt caatgtaacc ttgtatggac caaggtccgt
    16441 cgattacagc agcaacttta ccttcattaa acagacctct tagaacttgt ggattaccca
    16501 tatcaagagg attagaggga ttcgctttct ttaagcgttt catcgtctct agccctttct
    16561 tcgcttcaga tgaagcaatg cctagagctt taggatcgtt accggtttta ccgaattcgt
    16621 tggcaccata caaggtaata aacatacgtg aaacataata attttgtaca tcccatagca
    16681 gagcatattt atgctcggat gaattattaa acttttactg aaagtattga tttcttcgaa
    16741 tgtttttggt gcggtgttta acaaatcctt attatagaaa agcgccagcg ttgcgaaact
    16801 tacaggaaaa ccatagctga tgttattata tgtagctgcc gatactgcgc ctggaataaa
    16861 tgttttttta atccgctcag ctgagaccat gttttccata accccgccag caacgactaa
    16921 ccgccccagg gtatcatgtt caatttcagc tacatcagct acacgagtcg tacccccatc
    16981 ctgcataatt cttgatgctg catccattgg cgctatattg cgaaatgtaa actttacctt
    17041 gtagccaaaa tctttattga attcctttgc tgcatatttc atatattcca cgcttgtggc
    17101 atcagaccaa actaacaact cagcatcagt ttcaggaatt aatttttctg ccatcgcctg
    17161 gggtgttagc gtactggaat ttgcaagaat tataactgtg acgatatttg aaagcttcat
    17221 tatatttacc tcatgaataa catctttaag tagtatatga tgtacgacta cataagcatt
    17281 cacgccatta tcaacacaaa tcaaggccaa tcaactgtca aataagattt taatgatgaa
    17341 taactgcaaa tagtgatgta tatcccattt gcagtaattt gttgagaaaa aaatatttta
    17401 ttgccgcaga gtgtttagga gtacatgagg tgcgactata tggaataaca gaaatcatat
    17461 cttaaattga tattttggaa ggaactcatt tatggtttta ataaaaaaga atgtcttatc
    17521 ggcttcaatt gcaacagtca ttttaggctc aacaggagcc tttgcggcaa acgatcgcgg
    17581 ctttacctcc gacactgact ggtcagaaga acaagcgggc tttaatggac atgttggtac
    17641 tacatttgaa tatgagacca aaagcactga taactattta ggtaaaacag taaaggaata
    17701 tactaaaaca cacgagattt tccaggccta tttcagccaa cctaattggc aatttgcagc
    17761 aatctatggc cttaagtcta tcgatcgccg acaagaagaa aaaggctatc acgaaaatga
    17821 aacaagtctg caaaactata tatcactaaa caaaacgttc actattggta acggtttcga
    17881 cttcagtctg gtatacgacc tgatgtatac cgacggtaat atagatagcc cttatgtcac
    17941 aggcttgcat actgtaactg ttgacaacag ttttcgccct acgttgacgt actttaactc
    18001 tgactggaac gcaggttttt attcaaacgt tgaatacttg tacagtcgaa atgataagag
    18061 ctcctggggc gtacgcgaag aagaaggtca aagtatacta tttcagccct ataaacgttt
    18121 tggtgca
//
LOCUS       DAJTNA010000076        18048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTNA010000076 DAJTNA010000000
VERSION     DAJTNA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18048
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.077"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            36..353
                     /locus_tag="OO161_002159"
     CDS             36..353
                     /locus_tag="OO161_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HCR5463216.1"
                     /translation="MATDSQLTIETALNVGLALLGYFYIVFCSRRWLSLLFLKKWNKR
                     RKQDERQKAMNAFSEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTSRKNH
                     ISP"
     gene            307..621
                     /locus_tag="OO161_002160"
     CDS             307..621
                     /locus_tag="OO161_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463217.1"
                     /translation="MTITKQRVEKIIYRHEMGLNSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMDGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAASE
                     QD"
     gene            608..1153
                     /locus_tag="OO161_002161"
     CDS             608..1153
                     /locus_tag="OO161_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5463218.1"
                     /translation="MSKIDYQALREAAEKATKGKWAVEFDDEIYSTDGINHEQIAMVF
                     SENESRDAEFIAAANPATVLALLDERERNQQYIKRRDQENEDIALTVGKLRVELEAAK
                     SKLNEQREYYEGVISDGCKRIAELEAREVQLPTRYDLRYGHPINADERQVMIPKENGS
                     WLYLIDLEHALRVADIRIKGE"
     gene            1155..1418
                     /locus_tag="OO161_002162"
     CDS             1155..1418
                     /locus_tag="OO161_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463219.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPLPKKIGFYTIGDKTIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            1429..1941
                     /locus_tag="OO161_002163"
     CDS             1429..1941
                     /locus_tag="OO161_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5463220.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLA
                     AEPVAKIIAHYPLGVDVGKQKFVQAIRELPDFGGYLFAAPPAPIVPEEMYRQDAPVEG
                     SSKAAAYATGWNDCREAMLQSGNFRENKDSSTNNFRKIPEASTSSPVTPDGWISCSER
                     MPDDRQEVNQ"
     gene            2026..2268
                     /locus_tag="OO161_002164"
     CDS             2026..2268
                     /locus_tag="OO161_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5463221.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRITEHSVFFRRMDGRSGRVRIG
                     ELNCLFEHIDHQEYRKILADTEQEKHLKKLRAIKRK"
     gene            2425..2679
                     /gene="xisR"
                     /locus_tag="OO161_002165"
     CDS             2425..2679
                     /gene="xisR"
                     /locus_tag="OO161_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5463222.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            2713..3999
                     /locus_tag="OO161_002166"
     CDS             2713..3999
                     /locus_tag="OO161_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HCR5463223.1"
                     /translation="MSNASYPTGVEKHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            4020..4721
                     /gene="mlrA"
                     /locus_tag="OO161_002167"
     CDS             4020..4721
                     /gene="mlrA"
                     /locus_tag="OO161_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR5463224.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIRESK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGYDIFPLGI"
     gene            4781..4888
                     /locus_tag="OO161_002168"
     CDS             4781..4888
                     /locus_tag="OO161_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5463225.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(4869..5600)
                     /gene="yehW"
                     /locus_tag="OO161_002169"
     CDS             complement(4869..5600)
                     /gene="yehW"
                     /locus_tag="OO161_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5463226.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5605..6531)
                     /gene="yehX"
                     /locus_tag="OO161_002170"
     CDS             complement(5605..6531)
                     /gene="yehX"
                     /locus_tag="OO161_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5463227.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLEEA"
     gene            complement(6524..7681)
                     /gene="yehY"
                     /locus_tag="OO161_002171"
     CDS             complement(6524..7681)
                     /gene="yehY"
                     /locus_tag="OO161_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5463228.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDVIRRISTHPLLRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQIVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7688..8605)
                     /gene="osmF"
                     /locus_tag="OO161_002172"
     CDS             complement(7688..8605)
                     /gene="osmF"
                     /locus_tag="OO161_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5463229.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8816..11113)
                     /gene="bglX"
                     /locus_tag="OO161_002173"
     CDS             complement(8816..11113)
                     /gene="bglX"
                     /locus_tag="OO161_002173"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5463230.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNNYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVMNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            11309..13024
                     /gene="dld"
                     /locus_tag="OO161_002174"
     CDS             11309..13024
                     /gene="dld"
                     /locus_tag="OO161_002174"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5463231.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCHVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(13062..13994)
                     /gene="pbpG"
                     /locus_tag="OO161_002175"
     CDS             complement(13062..13994)
                     /gene="pbpG"
                     /locus_tag="OO161_002175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5463232.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTAPQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(14168..14755)
                     /locus_tag="OO161_002176"
     CDS             complement(14168..14755)
                     /locus_tag="OO161_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5463233.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14925..15503
                     /locus_tag="OO161_002177"
     CDS             14925..15503
                     /locus_tag="OO161_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5463234.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(15633..16394)
                     /locus_tag="OO161_002178"
     CDS             complement(15633..16394)
                     /locus_tag="OO161_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5463235.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNACAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(16447..17883)
                     /gene="mdtQ"
                     /locus_tag="OO161_002179"
                     /pseudo
     CDS             complement(16447..17883)
                     /gene="mdtQ"
                     /locus_tag="OO161_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
BASE COUNT         4541 a         4787 c         4567 g         4153 t
ORIGIN      
        1 tgttatgttc aaccagttac ttaaacaggt aagaaatggc aactgactca caactaacca
       61 tagaaacggc cctgaatgtc ggcctggcgc tccttggtta tttttacatc gtgttctgca
      121 gcagacggtg gctgtcgctg ttgttcctga aaaaatggaa taaacgccgt aagcaggatg
      181 aacgccagaa ggcaatgaat gcgttttccg aagccttcgg aattgacggc atggaaccag
      241 gggatccagc tcgcgcaatc agcagagggg gtgtagtaat ccttgtatat cggagtgaag
      301 agaaaaatga cgatcacaaa acaacgagta gaaaaaatca tatatcgcca tgaaatggga
      361 ctgaacagcg atgtcactgc cgaagaggtt tatgacctgg ctgtactggc gctgaattta
      421 tcaaatatcg caaacctgaa gcgatacgag cttgatatgg atggttgcga ctcgtgcggt
      481 caggattgtg gcgcagatat gactgaagat cctgatggtg attatgtcct gtttgatgac
      541 gtggttaagt tgtttgaatt tgatacaacc actcaaaagt tagaaatccc ggcaaaggag
      601 gctgccagtg agcaagattg actatcaggc actgcgtgag gcggcagaga aggctacgaa
      661 aggtaagtgg gctgttgaat tcgacgatga gatttactcc actgacggca taaaccatga
      721 gcaaatagct atggtgttca gtgaaaacga atctcgtgat gctgaattca tcgccgcagc
      781 caatccggct accgtcttgg cgcttctgga tgaacgggaa agaaaccagc aatacatcaa
      841 acgccgcgac caggagaacg aggatattgc gcttacggtt gggaagctgc gcgttgagct
      901 tgaagcagca aaatcaaaac tcaacgagca gcgcgagtat tacgagggag ttatctctga
      961 tgggtgcaag cgtattgctg aactggaagc gcgggaagtt caattaccga ctcgctacga
     1021 ccttcgatat ggacacccga taaatgcaga tgagcgacaa gtcatgatac ctaaagaaaa
     1081 tggcagttgg ctttacctga ttgacctaga acacgcatta cgcgtcgctg acattcgtat
     1141 caaaggagag tgatatggcc actttaacaa aaaaagaacg ggcatggttg aatgaattac
     1201 aggacgttct tgatcgctgt ccattaccaa aaaaaattgg tttttacacc attggcgata
     1261 aaaccattta cctgtatgac ctacgccgca tggatgaaat catggaggct cttgataatc
     1321 gttcgtcgat ggattggtgt gttgctgtcc atgatatgaa tgcagggttt gatgaaaaga
     1381 ttttgttccc ctcatcagtt gaaagcactg cgggttaagg agtaacacat gaccactata
     1441 accaaagagc gactgctgac aatcaagcag tggcgcgaaa catacggacc gggtagcaac
     1501 gttgtgcttc cagcagaaga agcggaagag ctggcacgga ttgcgctggc atcactggca
     1561 gcagagccgg tggcaaagat tatagctcat tacccattag gagttgacgt aggcaaacaa
     1621 aaattcgtac aggccattag agagcttcct gactttggcg gatatctatt tgccgcccct
     1681 ccagcgccga tagtgccgga agaaatgtat aggcaggatg cgccagttga aggcagcagc
     1741 aaagcggctg catacgctac aggctggaac gattgccgcg aagccatgct tcagtccgga
     1801 aactttcggg aaaataaaga ttcgtcaacc aataattttc ggaaaatccc ggaagcgtca
     1861 accagctctc cggtaactcc ggatggttgg ataagctgta gtgagcgaat gccggacgac
     1921 aggcaggagg tgaatcaatg agctggcctg atgcaatcgt aactctgggg gtggtattcg
     1981 cagcagcgtt tgttgtgttc tcgatttgtc gatggggata accacatgtt cgctttgatt
     2041 caacgcggtc agatatacac ggacagagcc ggataccccg tggtgattac tcgcatcact
     2101 gagcactcag tgttctttcg acggatggac ggacgatccg ggcgggtacg cattggtgag
     2161 ttaaactgcc tgttcgaaca tattgaccac caggagtacc gcaaaattct cgcggacact
     2221 gagcaggaaa agcacctgaa aaaattacga gccataaaaa ggaagtaaag aatgaataaa
     2281 gcatttgaac gatgggtcca ccagcgttac ggcaatcgct atgacctgac gcgagatgtt
     2341 gacggcttct actgtcgtga agttgtgaag cgaatgtttg acgtgtggtg ccactgccgt
     2401 ggatgaaaat tttatgaggt tggcatgcag acaatcatct atcagataac ccccagcaaa
     2461 tggtgtacgg agagagtcct cattgcatca acagggctaa agcctggcac cattgagcgg
     2521 gcaagaagaa agtcatggat gcagggaaaa gaataccgcc attacgctgt agaaggtgat
     2581 ccggggcact acagtgaatg cctgtacaac atcgaagaaa ttatgcgatg gatcgaaaac
     2641 cagaaacaac caggtgccaa aaatgcaagt tccggttaac ctgttaatgc tcctggacgt
     2701 ctgggaggtt taatgagtaa cgcatcatac ccgacaggcg ttgaaaaaca tggaggatca
     2761 ctccgtatat ggtttcacta taatggcaaa cgtgtcagag aaaacctcgg tgttcctgac
     2821 acagccaaaa accggaagat cgctggtgaa cttcgcactt ccgtttgttt tgcaatcaga
     2881 atggggagtt tcgactacgc cgcgcagttc cctaattccc ctaacctgaa acactttggt
     2941 ctgggaaaaa gagagataac cgttaaggca ctttcggaaa aatggttgga ccttaagaaa
     3001 attgagattt gtgcgaatgc acttaaccgt taccagtcag taattaaaaa catgttacca
     3061 atgttaggtg aaaaaaaact ggtttcatcc ataacaaaag aggatttact tttcgtaagg
     3121 agagatttgt tgaccggtta ccaaaagctt tctaatggaa agacttcttc cataaaaggg
     3181 cgctcagtgg tcacggtaaa ctactatatg acaaccatag ctggaatgtt tcaatttgca
     3241 acagataatg gttatacctc aggaaaccca tttaacggtc tggctccctt aaaaaagtcc
     3301 aaggtaaaac cagatcctct cacccgtgac gaatttattc gttttattga ggcttgccgt
     3361 catcaacaaa caaaaaacct gtggattctc gctgtataca cgggtattcg tcacggggag
     3421 ttggtatcgc tggcatggga agatatagac cttaaagcaa ggactataac catccgtaga
     3481 aattatacaa aacttggcga attcactcca ccaaaaaccg atgcaggcac cggaaggaca
     3541 attcatctgg ttcaaccagc tattgatgct cttaaaagcc aggcggaaat gaccatgctt
     3601 ggaaagcaac attctgtaga ggtgaagcag agggaatatg ggagaactgc tgtgcataaa
     3661 tgcacttttg tttttagtcc tcaggtaaca aaacagcagc agttgtccgg acctcactac
     3721 aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg tctgagacac
     3781 agaaaatcgt accaatccag gcatacttat gcatgctggt cacttgccgc aggagctaat
     3841 cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt caatgtttac
     3901 ggagcatgga tgaaagacaa caatcacgaa cagatagaac tccttaacaa aagactatct
     3961 gaaagtgtcc catgtatgcc ccataagaaa gctgggtaaa ataaaaactt gcaaaatcaa
     4021 ttagtttacc cttaatccct gtcacgttac gcgcgtggca gaggcgttac ggattgctga
     4081 aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt gaccggatcc
     4141 gcgagagcaa acgctggatc gacaacggcg tgcaggtcag caaagttaaa atgctgctca
     4201 gtaatgaaaa tgttgatgtg cagaacggct ggcgcgatca gcaagaaaca ttactgactt
     4261 acttgcaaag cggcaatctg catagcctgc gaacgtggat caaagagcgc ggtcaggatt
     4321 accccgccca gacgctcacc acgcatttgt ttattcctct gcgtcgacgg cttcagtgcc
     4381 aacaaccgac tctccaggcg ctgctggcga tcctcgacgg cgtactgatc aactacatcg
     4441 ccatctgtct ggcttcggca cgtaaaaaac agggtaaaga tgcgctggtg gttggctgga
     4501 atattcacga taccacccgt ctgtggctgg agggctggat tgccagtcaa caaggatggc
     4561 gcattgatgt cctcgcccac tcgctcaatc aactacgccc tgaactgttc gaaggccgta
     4621 cattgctggt gtggtgcggt gaaaatcgaa cctccgccca acaacagcaa ctcaccagtt
     4681 ggcaagaaca aggctatgat attttcccac tcggcattta atgattcgtt aacaaatgcg
     4741 ctttactgta caatcctttc gttaacataa ggagtgcatt atgcgcatag ctaaaattgg
     4801 ggtcatcgcc ctgttcctgt ttatggcgtt aggcggaatt ggtggcgtca tgctcgcagg
     4861 ttataccttt attttgcgtg ctggctaagc gcctgcacca gcctttcaaa caggcggtct
     4921 gcgatgatcg ccgccagtgc caccagtaac gccccctgga tcacatacgc ggtattaaat
     4981 ccgctaagcc cgatgatgat gggcgtaccc agcgtgctgg cccctaccgt tgaggcgatc
     5041 gtcgccgtac caatgttgat aatcaccgaa gttcgcacgc ccgccagaat caccggagct
     5101 gccagcggta gctcgacctt acgcagtcgc tgaccacgac tcattcccat acctttcgca
     5161 acttctgtca cgctggcatc aatcgctccc agcccggcaa gtgtcgcctg caggacgggc
     5221 agcacaccgt aaaggatcaa ggcgataatc gctggttgca gaccaaaccc gatcaccgga
     5281 acggcgatcg ccagcactgc gacgggcgga aaagtctgtc caacggcggc aatagtttcc
     5341 accagtgggc gaaattccgc gccccacggg cgagtgacag caattccggc accagtacca
     5401 atgatcaccg caaacaaact cgaaattccc accagccaga aatgagccag tgccagagcg
     5461 gcaaaacttt cttgctgata aacgggtcgt ggcagttgag ggaacaaggc agcaaacagc
     5521 ggctggctgt aaggcagcca gaaaatcagt gccacaaaca aagcaatgag ccagaacagc
     5581 ggatcgcgca acatcttcat acgcttacgc ctcctccagc agatcctgaa aatgcagcgt
     5641 gccgctaggc tggccctgcg tgttcaccac cggcagcacc tcgcatcccc gcgcgacaaa
     5701 cagggagagc gcatcgcgca gcgtcatctc ttctgccagt gcctcacctt ctgcccgttc
     5761 ttcgcgacgc acgtaatccg ccacactacg taacgaaagc aggcgcacac ccagttcact
     5821 acgtccaaaa aactggcgga caaaatcatt cgccggacga gtcagcatcg tcagcggatt
     5881 cccctgctgc accacttcac cgtgatccat caataccaga tgttctgcca gccgtagcgc
     5941 ctcatcaata tcatgagtga ccagcacaat ggtacgcccc agcaaacggt gaatgcgcgt
     6001 catctcttgt tgcaacgcgc cgcgcgttac cgggtccagt gcgccaaaag gttcatccat
     6061 cagtaagact tgcggatcgg cagccagtgc gcgcgctact cccacacgtt gctgctgacc
     6121 accggaaagc tgatgcggat aacgctcacg taaatttgac tccagcccca gtagcgccat
     6181 taattcgtcg atacgatcgt caatcctcgc ccgcgaccat ttttgtaatt gcggcacggt
     6241 ggcaatgttt tgtgccacgc tccagtgggg aaacaggcca atagattgaa tggcatagcc
     6301 catacggcgg cgcaactcca gtactggcag cgagcgaatt tcttctccgg caaagcggag
     6361 ctctccgctg tcatgttcca ccaggcggtt aatcattttc agggtggtgg atttgccgga
     6421 gccagatgtg ccaatcagca ccgaaaaact cccttcctga aaattgagat tgagatcgtt
     6481 aacggctttt tgtgcgccaa acagtttgct gacatggcta aattcaatca ttacgtttca
     6541 ccttcaacag tgcgataagt aaatcgaaca gcgcatcgat cagcaccgcc agaacaatta
     6601 ccgggatcac ccccagcaac actaaatcaa tggcgctgct tagcagcccc tggaaaacca
     6661 gcgcaccaaa accgcctgcg ccgattaacg ccgcaatcac cgccatgcct acaatttgca
     6721 ccatcaccac ccgcaggctg cgcagaaata ccggtaacgc cagcggtaac tgaacatgca
     6781 ggaatcgctg cgccccgctc atccccatcg ctctggcgct ctccagcaca tcgcgcggga
     6841 tctggttcaa gcctactacc acgccgcgca ccagcggcag caaggcatag agcaccagcg
     6901 caatcagtgc gggtgtcatt ccggttcctg ctatgccgag cttccccagc cacggaaagg
     6961 ccgtcaccag cgcggcaagc ggcgcaatca acaggccaaa gagcgccacc gaaggcacgg
     7021 tctgaatgac attgagcaga gaaaaaatcg ccccctgccg cgcggtggaa aagtagcacc
     7081 agatgcccaa tggcacacca atcactaacg caggcagcac cgcaccaaac agcaacgtca
     7141 gatgttgagc cagcgcgtcg tcaaacacat cctgacggtt ggcgtattct ttcattagcg
     7201 agagatcgtt aagcgtgccg gagtaaagca accacagcgg aataatggca atctgcatat
     7261 gcaacaacca gcgcaacagc ggatgcgtgg agattcggcg gatgacatcg ctacaggcca
     7321 gcaatgccag cgccgcagcc agccagaaac cactgccgag gctggtacgc gccagcgcac
     7381 tgccattttg cgccagttgg gtcgccgcct ttccagctcc ccacaccagc aatacgaaga
     7441 cgaattgcgc cagaatgagt gcaaaaatgc tgcctttttt agcgggaata aaacaggctg
     7501 tcagccaggc gcaaccaacg cccaccagca tccagatcgt ttgcggccac agttgccaga
     7561 gatgacgccc ttcacccgag actaaacgat taggcgcgta gctgataaac ggcagcgctg
     7621 ctgcgattgc cgtcagcaac agcagcagcg ccagaacagg attaatacgt agataagtca
     7681 cgggaaatta cttcgcccac cctttttgtt tcaggtagtc ggcagccact tttttggcat
     7741 ccagtccttc cactgcaatg ctggcattca gttgctgcaa tgtttttgca tcgaggctgg
     7801 cgaagactgg ctgtagccac tgtgccattt gcggatactc tttcagcacc gactcacgca
     7861 ccactggtgc aggcgcgtag ataggttgca cgccttgcgg atcgcttaag gtttgcagcc
     7921 ccagcgccgc gaccggaccg tcagtgccgt aagccattgc ggcattaacg ccagaagttt
     7981 gctgggcagc ggctttaatc gtcaccgccg tgtcgccacc agccagtgac agcaactgat
     8041 cctgaccgag cttaaagcca taggcttttt caaacgcggg taaggcatcg gcgcgttcga
     8101 taaactctgc cgaggctgcc agtttgaagg tgccgccctc tttcagataa cgactcaggt
     8161 cagcaagcga agtgagtttg tttttctctg ccacatcctg acgcacggcg atggtccagg
     8221 tgttatttgc aggtgcgggc gtcagccaga ttaacttgtt ttgctctgca tcgagttttt
     8281 tgactttctc gtaaccttgc tgcgcgtttt tccacgctgc atcgttttca tctttaaaga
     8341 aaaacgcgcc attgccggta tattccggat agatatccag ttcaccagaa gtaatcgccc
     8401 cgcgcaccac aggagtcgtt ccgagttgca ctttattgac cgtcggtacg ccgtggcttt
     8461 ccagtacctg caaaatgata ttgccgagca gcgcgccttc agtatcgatt tttgaaccga
     8521 ctttaacggg ggaagccgcg tgcagcggca ggctcacggc tgccaacaaa accagtgaac
     8581 ctgcccagac ctttgagagt ggcatgatgc tttcctcatt cttttactgt tgttttccgc
     8641 gaattaagag aaaagcatag ttgataatgg cgggattagc ctgagcggac ggattcagtt
     8701 gcagaatcag ataaatactt aagaggcata ttcggtccgg cgtttcctgc cggatgcggc
     8761 gcgagcgcct tatccggcct acaaagggcg caaatgtcac gcccttacta aagcattaca
     8821 gcaactcaaa ctcgcctttc ttaacgcgtg cagaatcagt gccgataaag acattgaact
     8881 tgccaggctc ggcgtcatat ttcatctgtt gattccagaa cttcagcgcc tcgatatcaa
     8941 tcgggaagct aacggtctga gtttcgcccg gtttcagggt gattttctca aagcctttca
     9001 gctgtttcac cggacgactc atggaagccg tcacatcctg caagtacatc tgcactaccg
     9061 tggccccttc gcgtttaccg gtgttcatca cctgcacgct ggccgtcact ttgccgtcac
     9121 gcttcatggt cggcgcagaa agtttcacat cagagacggt gaaagtggta tagctcagac
     9181 catagccgaa cggataaagc gccccgttag cttcatcaaa ataacgcgaa gtgtatttgt
     9241 tcggcttgtc ggcattatac gggcgaccgg tgttcagatg gctgtagtac accgggatct
     9301 gcccgacaga acgcgggaag gacatcggca gcttgccgga cgggttgtaa tcgccaaaca
     9361 acacatcggc aattgcatta ccgccttcag tccccgcaaa ccaggtttcc agaatcgcat
     9421 cagcctgctg atcttctttc accagcgcca gcggacgccc gttcatcagc accagcacca
     9481 gcggtttacc ggtggctttc agcgccgcaa tcaagtcacg ttggctttgc ggaatagtaa
     9541 tatcggtacg gctggaggcc tcgtgcgcca tcccctgtgc ttcaccgact acagccacca
     9601 ccacatcaga ttgtttcgcc gtctgcaccg cttcatcaat catctcttgc ggcgaacgcg
     9661 gatcgacttt gaccgcttct tcatactgat tcaggaaatc gataatgcct ttgtcactgg
     9721 taacgttcgc ccctttggca tacagcactt taccgttttc accgacggca tttttaatcc
     9781 cggtcagcac ggtcacggat tgatctgcaa cacctgccgc cgaccagctt cccatcacgt
     9841 cacgcttgct gtctgccagc gggccaacca ccgcaatggt tgcggatttt ttcagcggta
     9901 acgtttcgag acggtttttc agcaacacca ggctttcacg cgccacttca cgcgcttctt
     9961 tgcggtgcaa gcggctttcg gcattggtat ccaccgggtc agactctttc ggaccgagat
    10021 ggctgtatgg gtcgttaaac aaccccatat catatttaac gttcagtaca tggcgggcag
    10081 catcgtccag ctctgccatc gtcactttac cggatttaat caacccaggc agatacttcg
    10141 agtagtactc gtcgctcata ctcatgttga ttccggattt cagcgccacg cgcaccgcat
    10201 cttccgggtc tgccgccgtg ccatgtttaa tcagctcttt gattgcaccg tgatcggaaa
    10261 cggtgatgcc tttaaagccc cactggtcgc gcagaacatc tttcagcagc caggaatcgg
    10321 aggtggctgg cgtgccgttc agcgagttca gcgccaccat caccgcgccg ctgcctgcgt
    10381 ccagccccgc tttgtacggc ggcatataat tattaaacag gcgctgcgga ctcatatcga
    10441 cggtgttgta ctctttaccg ccttctaccg cgccgtatgc ggcaaagtgt ttgacgctgg
    10501 tcatcaccga gtagcgatct gccgggcttt taccctgcat cgcttccacc atggttttac
    10561 ccattgttga ggtgagatac gtatcttcgc caaaaccttc ggaagcacgt ccccagcgcg
    10621 gatcgcgcga gacatcgacc atcggtgccc aggtcatatt caggccatca tctgccgctt
    10681 cataagcaga gacacgtccg accgttttca ctgcatcgag gttaaaagac gaggccagac
    10741 cgaggctaat cgggaacacc gtgcgctgac cgtgcagcac gtcgtaagca aagaaaagag
    10801 gaattttcag gcggcttaat tccatcacct gatcctgcat ggcgcggata tcctgacggg
    10861 ttacggtgtt gaaaatcgcc ccaacctgac cgtctttgat catctcgcgg atcgcctctt
    10921 tcgggttatc cgggccgacg ctgattaagc gcagctgacc aattttctca tcaactgtca
    10981 ttttcttaag cagttcggtg acgaacgcat cccgcgcttc gggcgttaat ggatggttgc
    11041 cgaataaatc atccgccagt gctggctgca gggccagact caccgcgatt cctactgaac
    11101 atagccattt catatggatt tttcttcctc tgttgcccga cgaggcagcg aaaatggtgc
    11161 aaaaaccgta gtttgccata agcatgatgg agagagaaaa agaatgctca gtttattgtc
    11221 tgaattttca aaatattcac tcgctgaatt gtcatacaag gcgctattct agtttgtgat
    11281 attttttccc caccacaagg agtggaaaat gtcttccatg acaacaactg ataataaagc
    11341 ctttttgaat gaacttgccc gtctggtcgg tcattcacac ctgctcaccg atcccgcaaa
    11401 aacggcccgc tatcgcaagg gcttccgttc tggtcagggc gacgcgctgg ctgtcgtttt
    11461 ccctggctca ctactggaat tgtggcgggt gctgaaagcc tgcgtcaccg ccgacaaaat
    11521 tattctgatg caggctgcca atacaggcct gaccgaagga tcgacgccaa acggtaacga
    11581 ctatgatcgc gatatcgtga tcatcagcac cctgcgtctc gacaagctgc acgttctcgg
    11641 caagggcgaa caggtgctgg cctatccggg caccacgctc tattcgctgg aaaaagccct
    11701 caaaccgctg ggacgcgaac cgcactcagt gattggatca tcgtgtatag gcgcatcggt
    11761 catcggtggt atttgtaaca actccggcgg ctcgctggtg caacgtggcc cggcgtatac
    11821 cgaaatgtcg ttattcgcgc gtataaatga agacggcaaa ctgacgctgg tgaaccatct
    11881 ggggattgat ctgggcgaaa cgccggagca gatccttagc aagctggatg atgatcgcat
    11941 caaagatgac gatgtgcgtc acgatggtcg tcacgcccac gattatgact atgtccaccg
    12001 cgttcgtgat attgaagccg acacgcccgc acgttataac gccgatccgg atcggttatt
    12061 tgaatcttct ggttgcgccg ggaagctggc cgtctttgca gtacgtcttg ataccttcga
    12121 ggcggaaaaa aatcagcagg tgttttatat cggcaccaac cagccggaag tgctgaccga
    12181 aatccgccgt catattctgg ctaacttcga aaatctgccg gttgccgggg aatatatgca
    12241 ccgggatatc tacgatattg cggaaaaata cggcaaagac accttcctga tgattgataa
    12301 gttaggcacc gacaagatgc cgttcttctt taatctcaag ggacgcaccg atgcgatgct
    12361 ggagaaagtg aaattcttcc gtccgcattt cactgaccgt gcgatgcaaa aattcggtca
    12421 cctgttcccc agccatttac cgccgcgcat gaaaaactgg cgcgataaat acgagcatca
    12481 tctgctgtta aaaatggcgg gcgatggcgt cggtgaagcc aaatcgtggc tggtagatta
    12541 tttcaaacag gccgaaggcg atttctttgt ctgtacgccg gaggaaggca gcaaagcgtt
    12601 tttacaccgt ttcgccgctg cgggcgcagc aattcgttat caggcggtgc attccgatga
    12661 agtcgaagac attctggcgt tggatatcgc tctgcggcgt aacgacaccg agtggtatga
    12721 gcatttaccg ccggagatcg acagccagct ggtgcacaag ctctattacg gccattttat
    12781 gtgccatgtc ttccatcagg attacatagt gaaaaaaggc gtggatgtgc atgcgttaaa
    12841 agaacagatg ctggaactgc tacagcagcg cggcgcgcag taccctgccg agcataacgt
    12901 cggtcatttg tataaagcac cggagacgtt gcagaagttc tatcgcgaga acgatccgac
    12961 caacagcatg aatccgggga tcggtaaaac cagtaaacgg aaaaactggc aggaagtgga
    13021 gtaaaaatta cggatggcag agtatcgcca tccgaattca cttaatcgtt ctgtgccgtc
    13081 tgccccgccg ccgccatttg ggcggctttt tgttttttat agctcaacgc tgctgccggc
    13141 acaggcatca ctttaccggt ttcaatccag gtacgcaggc ggctggcatc ggcaaaatgg
    13201 gtatatttgc caaacgcgtc catcactacc agcgccaccg gtttattatt gataaccgta
    13261 cgcatcacca gacaatggcc cgccgcattg gtaaagccgg ttttggttaa ctgaatattc
    13321 cagttatcgc gatacaccag atgattagta ttgcggaacg gcagcgtata cgtcgggtta
    13381 gagaaggttg ccatatcttc ccgggtagta cttaactgcc cgatcaacgg atattgtttg
    13441 ctggcaatga gcagtttggt taagtcacgg gcagttgaaa cgttatgcac cgacaatccg
    13501 gtaggttcaa caaagcgcgt gttgttcatt ccgagcgatt tcgctttcgc attcattgcc
    13561 ttaataaagg ctttgtaacc accgggataa tggtgcgcaa ggcttgccgc cgcgcggttt
    13621 tctgaagaca tcagcgccag caacagcata tctttacggc tgatttcgct attcagtcgt
    13681 acgcgcgaat agaccccttt catctccggc gtctggctga tatccacttt tagtttttca
    13741 tccagcggca gtcgtgcatc cagcacaacc atcgcggtca ttaatttgct gatagacgca
    13801 atcggacgca ccagatccgg gtggttcgaa tagatcactt tgttggtatt cagatcaaca
    13861 atcatcgcgc taccggaggc aatttccggt tgtggagcgg tggtagcggc tgccgttttc
    13921 gcaacggcct gcggtgccaa aggcacagcc agcatcaggg ccaggctaaa taaagaaact
    13981 cgaaatttcg gcatgatgag cattcagata gtggttcacg cgcacgggtt gcgcaccgcc
    14041 ggagtaagga tttactgagg ctagcgacgc catcataacg agcaaaaagt gcgatcgtca
    14101 aaggagaatc gtgaggaaat gctgcattgc tgacatttac gccagcaatg caacgtcaaa
    14161 gaactttcta gaacaaacga taaccgtagc cccatagtat aacggttagg gcgagcagca
    14221 cttccagtac cagcacgcca atcgccagcg tcgaactaga gaagctaagg ccttcctctt
    14281 tattgatatt caggaagctc ggaataccaa ggtaaagcag gtagccggtg taaaacagcg
    14341 ccaccgtgcc gaccagcgca cacaaccaga ccagcggata aagcgccacc agaccgctta
    14401 aaaacagcgg agttgcaacg tagcccgcga agaccatgca gtgcgcaagt gacggacgct
    14461 gcggataatt acgcgccatc caccagatga cccgccccat taccgcaacc ccagccagca
    14521 taacgccata aaacaggaca gccagcgcca gtccggtaaa ccaggataac ttcaggatag
    14581 tgccatcgcc aaaattccag ccaatctgtg tagtgccaat gaaggcgcaa atcaccggaa
    14641 tcgccgccat cagcaaaacg tggtgggtgt aatggtgaga aatcgtttcg ttttcgcgat
    14701 taatcacctg catttcacga tcgggatggg aaaacagtcc ccagacatgg ctcataccgc
    14761 ccccttgttg tgagttcatg aacctgacag ttcaagtata agtcagcttg tgattatttt
    14821 ttgttcatcc atgtaatttg caccgtctga tgtatgtcct ttttcagcgt gaatgatttc
    14881 cagggtgtat gcttacaggc aaccaaaggg agacagactg gcctatggat ctcaatacac
    14941 tcatctcaca atatggttat gccgcgctgg tgatcggtag cctggcggaa ggtgaaaccg
    15001 tgactttgct gggaggcgtt gcggcacatc aggggctatt aaagttcccg ctggtggtac
    15061 tttctgtggc gcttggcggc atgattggcg accaggtgct ttatctgtgc gggcggcggt
    15121 ttggcggcaa gctgttacgc cgtttctcga aacatcagga taaaattgag cgggcgcaga
    15181 aacttatcca acgccatccg tatctgtttg tcattggtac gcgctttatg tatggctttc
    15241 gggtgattgg cccgacgctg attggtgcca gccagctgcc gccgaaaatc tttctgccgc
    15301 tgaatattct cggcgcattt gcctgggcgt tgatttttac cactattggt tacgctggtg
    15361 gtcaggtgat tgcgccgtgg ttgcacaatc tcgaccagca tttgaagcac tgggtctggt
    15421 tgattctggt cgtggttctg gtggtgggcg tgcgctggtg gctgaaacgg cgcgggaaga
    15481 aaaagccgga tcatcaggcg taaaaccatt gccctgggta aggcgttcgc gccgcatccg
    15541 acatccggtg caccatgcct gatgcgacgc ttgtgcgtct tatcatgcct accaaaattt
    15601 tacccattga aataaccacg cacctcactg cgctattctg ggttgaactg cggattcgcc
    15661 aacataaagc cgccatccac tatcaacgac tgcccggtgg tgtaatttgc gccctctgag
    15721 cacagccacg ctaccaggct ggcaatctca tgcgttgcgc caaaacgccg cagaggaatc
    15781 gaaggctccg cgtcgggctt cacgtcgctg tcatccatgc cattcattgg cgtggcgatc
    15841 gccccaggcg cgactgcgtt caccaaaatc ttatgcctga ccagctccag cgccatcgct
    15901 ttggttaacc caccgagcgc atgtttagcg gctgtgtagg cgctggcatc cggcagcggc
    15961 gtatgttcat gtaccgacgt aatgttgatg atgcgaccgc cctgcccttg tttcaccatc
    16021 tggcgggccg caatttgtga gcataaaaat gcaccatcga catcaacggt aaaaatcttg
    16081 cgccactcat cgaaagccat atcaagaaac ggcgctttgg tcattgcgca cgcattattc
    16141 accagcacat caatacgccc cagccgttga atgagtttct ccagcgcctg cgcaccttct
    16201 ggcagtttac cgagatccag ctgcacaatc tccgcacgta cgccgtggct aactacctca
    16261 cgcgcagtat cttttgcccc ttcttcatct gagtgccagg taataccaat atcaaacccc
    16321 tgctgcgcca gtaataacgc gcactctttg ccgatccccg aatcggaggc ggtaataatc
    16381 gcaacctgtg ccatcgagtt ctccacttaa cgctgaataa acgttaagta tagaaggcgc
    16441 atatcatcag cgtttgtaac ccccgcccaa cgcaccagtg agttgaatgg aagcatccag
    16501 ccactgccct tgcaataaca ggccattggc ccgctcacgc agcgcgggga ttctggcttc
    16561 gctgacgcgg gaaccagcaa tgatgcccgc gttaaagcgc gcctgcgcaa gacctaccac
    16621 acgcaaggca tcgcgctcaa tttgcgcctg atgctggttt ttctccgcaa gtgtctgaac
    16681 ctgactggct gcccgcgcca cgtcattcac cgcttcaacc accgctttgt tgtagctggt
    16741 gatagacaag ttgctttcgg cttttgcgat atcgagattg gcgttaagac gaccactatc
    16801 gaaaataggt agcgtcaggc ctgccgtaac gcccatttgc tgcgcggaat gacggaacag
    16861 atcgcttaag tgcaacgcat cctgttgcag gaaggccatc aggttgatgt caggataaaa
    16921 tgccgctttt gccgcatcaa tggtgcttag cgatgactca acgtaccagt gcgccgcctg
    16981 caaatctgcc cgccgggcca gtaaggagta ccccagttca tcaggaagct ggcttgccac
    17041 tttcggcaac gcgaccggtt taagcttcaa tgactttgtc tggtgatttg taagtgcgct
    17101 taaccgtgcc tcaataattt tcattttccc cgcgacatcg ttgagctgct gccgggtttt
    17161 gctggcatta atatcggttt ccacaccgtc aactgaagaa gtaatcccgt tctgatatag
    17221 ctggcgatcg gtcgcgataa tggtgttctg ctctttttct atttgctgca agaccgtgtt
    17281 taacgccgcc tgggtttgcc actcccagta caggcgggct acgctgccag ccagcaattg
    17341 gcgggtttgc tcgcgttccg ccgcccgtgc tttaaccgta cccaggcggg cagtaacctc
    17401 cgcccgattc tttccccaga tatcgagatg ccagcccgcc gttaagccaa aagttccgtt
    17461 ggtgtaccac ggaccggtcg tacctgcggc cggatcgttc agagcaaacg gccccattag
    17521 cccttctgcc gacatttttt gccgctccat atccgcctaa aagtcgatct gcggaccatc
    17581 ctgagtggca actgccttcg cctgggcttc agctagctga atgcgctgtt cagccacctg
    17641 catatccggt gcgttctgta gtgcattgtt aattaatgaa gtgagctgat tatcgtgata
    17701 ctccagccac cattggctgt ctggccaacc atttttcagc gccgtgggta atgcggtgtc
    17761 aacttgtgca gcgggcgttt gctggcttaa cgcctggcgg gtttcatgca taggcgcaca
    17821 cccggccagc atcagtaaca gcggaaaaca ggcgatggct ggataaaagg aatcacgatt
    17881 catgggggaa taatcaggta agaaacggtg cgcggtgatt accgtgtgtt gcgatatatt
    17941 ttttagtttc gcgtggcaat acatcagtag caataaaacg acatacccag aaaaatatag
    18001 actaagtgat tgatattctc cgatttatct taatcactta taaatgac
//
LOCUS       DAJTNA010000077        18008 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTNA010000077 DAJTNA010000000
VERSION     DAJTNA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18008
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.078"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            58..134
                     /locus_tag="OO161_002180"
     tRNA            58..134
                     /locus_tag="OO161_002180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92..94,aa:Arg,seq:acg)
     gene            415..981
                     /gene="yqaB"
                     /locus_tag="OO161_002181"
     CDS             415..981
                     /gene="yqaB"
                     /locus_tag="OO161_002181"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5463236.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQTIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            978..1391
                     /locus_tag="OO161_002182"
     CDS             978..1391
                     /locus_tag="OO161_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5463237.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFCALVKRYAMLQLQQLPFRA"
     gene            1478..3034
                     /gene="gshA"
                     /locus_tag="OO161_002183"
     CDS             1478..3034
                     /gene="gshA"
                     /locus_tag="OO161_002183"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5463238.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            3184..3699
                     /gene="luxS"
                     /locus_tag="OO161_002184"
     CDS             3184..3699
                     /gene="luxS"
                     /locus_tag="OO161_002184"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5463239.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3763..5301)
                     /gene="emrB"
                     /locus_tag="OO161_002185"
     CDS             complement(3763..5301)
                     /gene="emrB"
                     /locus_tag="OO161_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5463240.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPDAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5318..6490)
                     /gene="emrA"
                     /locus_tag="OO161_002186"
     CDS             complement(5318..6490)
                     /gene="emrA"
                     /locus_tag="OO161_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR5463241.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVAANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6617..7147)
                     /gene="emrR"
                     /locus_tag="OO161_002187"
     CDS             complement(6617..7147)
                     /gene="emrR"
                     /locus_tag="OO161_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5463242.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7238..7573)
                     /gene="ygaH"
                     /locus_tag="OO161_002188"
     CDS             complement(7238..7573)
                     /gene="ygaH"
                     /locus_tag="OO161_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5463243.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7563..8300)
                     /gene="ygaZ"
                     /locus_tag="OO161_002189"
     CDS             complement(7563..8300)
                     /gene="ygaZ"
                     /locus_tag="OO161_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5463244.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRYVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8424..9608)
                     /locus_tag="OO161_002190"
     CDS             complement(8424..9608)
                     /locus_tag="OO161_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5463245.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9900..10892)
                     /gene="proX"
                     /locus_tag="OO161_002191"
     CDS             complement(9900..10892)
                     /gene="proX"
                     /locus_tag="OO161_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5463246.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10950..12014)
                     /gene="proW"
                     /locus_tag="OO161_002192"
     CDS             complement(10950..12014)
                     /gene="proW"
                     /locus_tag="OO161_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5463247.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTTLLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(12007..13209)
                     /gene="proV"
                     /locus_tag="OO161_002193"
     CDS             complement(12007..13209)
                     /gene="proV"
                     /locus_tag="OO161_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5463248.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13564..14523)
                     /gene="nrdF"
                     /locus_tag="OO161_002194"
     CDS             complement(13564..14523)
                     /gene="nrdF"
                     /locus_tag="OO161_002194"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5463249.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KNFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14533..16677)
                     /gene="nrdE"
                     /locus_tag="OO161_002195"
     CDS             complement(14533..16677)
                     /gene="nrdE"
                     /locus_tag="OO161_002195"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5463250.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16650..17060)
                     /gene="nrdI"
                     /locus_tag="OO161_002196"
     CDS             complement(16650..17060)
                     /gene="nrdI"
                     /locus_tag="OO161_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5463251.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFSEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(17057..17302)
                     /gene="nrdH"
                     /locus_tag="OO161_002197"
     CDS             complement(17057..17302)
                     /gene="nrdH"
                     /locus_tag="OO161_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5463252.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17550..17879)
                     /locus_tag="OO161_002198"
     CDS             complement(17550..17879)
                     /locus_tag="OO161_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5463253.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALMKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
BASE COUNT         4482 a         4587 c         4688 g         4251 t
ORIGIN      
        1 cttgatacgg ctttagtagc ggtatcaaaa aatctgcagt aaagtaagtt tcccgatgca
       61 tccgtagctc agctggatag agtactcggc tacgaaccga gcggtcggag gttcgaatcc
      121 tcccggatgc accatattct ccgtaacctt cagcaatgaa ggtatcgaag agatagcggt
      181 attaaccgca ggcactaggg aggataacgt tgcgtcagca acggcccgaa gggcgaacgc
      241 cagtgagtca tcctcccgga tgcaccatct tctcccaaaa atttccttca aaaatatctc
      301 atttctaaat aactacttta tccatttcct gaattttctt ctccttcgcc atcagcgaca
      361 aaatcaagca attacgctaa agtagcgtca tatttttcga cttgcgagaa gaccatgtac
      421 gagcgttatg caggtttaat ttttgatatg gatggcacaa tcctggatac ggagcctacg
      481 caccgtaaag cgtggcgcga agtattaggg cactacggtc ttcagtacga tgttcaggca
      541 atgattgcgc ttaatggatc gcccacctgg cgtattgctc agacaattat tgagctgaat
      601 caggccgatc tcgacccgca tgcgttagcg cgtgaaaaaa cagaagcagt aagaagtatg
      661 ctgctggata gtgtcgagcc gcttcctctt gttgaagtgg tgaaaagttg gcatggtcgt
      721 cgcccaatgg ctgtaggaac ggggagtgaa agcgccatcg ctgaggcatt gctggcgcac
      781 ctgggattac gccgttattt tgacgccgtc gtcgctgctg atcacgtcaa acaccataaa
      841 cccgcgccag acacattttt gttgtgcgcg cagcgtatgg gcgtgcaacc gacgcagtgt
      901 gtggtctttg aagatgccga tttcggtatt caggcggccc gtgcagcagg catggacgcc
      961 gtggatgttc gcttgctgtg agtgaagcgt tatcgctttt ctcgttgttt gccagtagtt
     1021 ttctcagcgc tacactatta cccggcaact ccgaagtcgt gctggtggca atgttgcttt
     1081 ccgggatcag ttatccctgg gttttagttt taacagcaac aatgggtaat agccttggag
     1141 ggttaactaa cgttatcctt gggcgtttct ttccattgcg taaaacatcg cgctggcaag
     1201 agaaagctac tggctggctg aaacgctatg gtgcagtcac gctattatta agctggatgc
     1261 ccgtggttgg cgatttactg tgtctgttag cgggatggat gcgcatctcg tggggaccgg
     1321 taatcttttt ttgtgccttg gtaaagcgtt acgctatgtt gcagttgcag cagctaccgt
     1381 tcagggcatg atgtggtggc actaattgta ggcctgcaca tatggtcacc attacagtta
     1441 tgctaattaa aacgattttg acaggcggga ggtcaatttg atcccggacg tatcacaggc
     1501 gctggcctgg ctggaaaaac atcctcaggc gttaaagggg atacagcgtg ggctggagcg
     1561 cgaaactttg cgtgttaatg ctgatggcac actggcaaca acaggtcatc ctgaagcatt
     1621 aggttccgca ctgacgcaca aatggattac taccgatttt gcggaagcat tgctggaatt
     1681 cattacacca gtggatggtg atattgaaca tatgttgacc tttatgcgcg atctgcatcg
     1741 ttatacggcg cgcaatatgg gcgatgagcg gatgtggccg ttaagtatgc catgctacat
     1801 cgcagaaggt caggacatcg aactggcaca gtacggcact tctaacaccg gacgctttaa
     1861 aacgctgtat cgtgaagggc tgaaaaatcg ctacggcgcg ctgatgcaaa ccatttccgg
     1921 cgtgcactac aatttctctt tgccaatggc attctggcaa gcgaagtgcg gtgatatctc
     1981 gggcactgat gccaaagaga aaatttctgc gggctatttc cgcgttatcc gcaattacta
     2041 tcgtttcggt tgggtcattc cttatctgtt tggtgcatct ccggcgattt gttcttcttt
     2101 cctgcaagga aaaccaacgt cgctgccgtt tgagaaaacc gagtgcggta tgtattacct
     2161 gccgtatgcg acctctcttc gtttgagcga tctcggctat accaataaat cgcaaagcaa
     2221 tcttggtatt accttcaacg atctttacga gtacgtagcg ggccttaaac aggcaatcaa
     2281 aacgccatcg gaagagtacg cgaagattgg tattgagaaa gacggtaaga ggctgcaaat
     2341 caatagcaac gtgctgcaga ttgaaaacga actgtatgcg ccgattcgtc caaaacgcgt
     2401 tacccgcagc ggcgagtcac cttctgatgc gctgttacgt ggcggcattg aatatattga
     2461 agtgcgttcg ctggacatca acccgttctc gccgattggt gtagatgaac agcaggtgcg
     2521 attcctcgac ctgtttatgg tctggtgtgc gctggctgat gcaccggaaa tgagcagtag
     2581 cgaacttgcc tgtacacgcg ttaactggaa ccgggtgatc ctcgaaggtc gcaaaccggg
     2641 cctgacgctg ggtatcggct gcgaaaccgc gcagttcccg ttaccgcagg tgggtaaaga
     2701 tctgttccgc gatctgaaac gcgtcgcgca aacgttggat agcattaacg gcggcgaagc
     2761 gtatcagaaa gtgtgtgatg aactggtcgc ctgcttcgat aatcccgatc tgactttctc
     2821 tgcccgtatc ttaaggtcta tgattgatac tggtattggc ggaacaggca aagcgtttgc
     2881 agaagcctac cgtaatctgc tgcgtgaaga gccgttggaa attctgcgcg aagaggattt
     2941 tgtagccgag cgcgaggcat ctgaacgccg tcagcaggaa atggaaaccg ctgataccga
     3001 accgtttgcg gtgtggctgg aaaaacacgc ctgacagaaa agaaaaaggc cactcgtgag
     3061 tggccaaaat ttcatctctg aattcaggga tgatgataac aaatgcgcgt ctttcatata
     3121 ctcagactcg cctgggaaga aagagttcag aaaattttta aaaaaattac cggaggtggc
     3181 taaatgccgt tgttagatag cttcacagtc gatcataccc ggatggaagc gcctgcagtt
     3241 cgggtggcga aaacaatgaa caccccgcat ggcgacgcaa tcaccgtgtt cgatctgcgc
     3301 ttctgcgtgc cgaacaaaga agtgatgcca gaaagaggga gccatactct ggagcacctg
     3361 tttgctggtt ttatgcgtaa ccatcttaac ggtaatggtg tagagattat cgatatctcg
     3421 ccaatgggct gccgcaccgg tttttatatg agtctgattg gtacgccaga tgagcagcgt
     3481 gttgctgatg cctggaaagc ggcaatggaa gacgtgctga aagtgcagga tcagaatcag
     3541 atcccggagc tgaacgtcta ccagtgtggc acttaccaga tgcactcgtt gcaggaagcg
     3601 caggatattg cgcgtagcat tctggaacgt gacgtacgca tcaacagcaa cgaagaactg
     3661 gcactgccga aagagaagtt gcaggaactg cacatttagt cagtaaacta tcttcacaat
     3721 taattgaaaa aagccagttc aaatgaactg gcttagttgt acttagtgcg caccacctcc
     3781 gccgccacct gcgccaaagg gcggtttagc aaaccacacc agccccagca ggacgaggaa
     3841 tatcccggct gacatccaga agatctcatt ggcggaaata atcagcccct gattagtgat
     3901 ctgctgggca atccagcctg atgcctgctg ttgcgtcatc ccaagccctt ccagttgact
     3961 gtacatcgcc tgggcatccg ggttgaatgg gtttaccgac tcagtcaact gcgcatggtg
     4021 catcgactcg cggttggtcc acatggtcgt ggttatcgac gtgccgatag accccgccag
     4081 cgttcgcgta aagttagaga ggctcgatgc cgccgccagt cgttccggtg gcaaaccaga
     4141 cagcgtaatg gtggtcagcg gcataaagaa gcaggccacc gcaaacccct ggataaactg
     4201 cggccaggcc gacgcgccaa aatccatacc tggttcaaag gtataggcac gccagtagaa
     4261 gcagacggca tacataataa agctgaaggt taccagccgc cgcatatcca gtttatgcgc
     4321 gaagcggccg ataatcggcg acaggatcac cggaataatc cctaccggcg cagaggccaa
     4381 acctgcccag gtcgccgtgt aaccgtagac ctcctgcaac aactgcggca gcagaacaat
     4441 cgcgccgaag tagagcatat acgcgaggct gatacacaag cagccgatgg tgaagttgcg
     4501 cgacttaaac aacgacagat cgactatcgg gttatcgtcg gtcagctccc agacaatcag
     4561 gaagcagata gccaccaccg ccaccacggt aagaatgata atttcctgtg atgaaaacca
     4621 gtccagctct ttaccgcggt cgagcataat ctgcaggcta ccgataccaa taaccagcag
     4681 tgccagcccc acggcatcaa tccgccgccg ttcggtgcgg gtttcgcgtc cgcgcagggt
     4741 ttgcagtgtc atcaacacca ccgccacgcc aattggcacg ttgatgaaga atatccagcc
     4801 ccagtggtaa ttatcgctga tataaccgcc gaggattggg ccgcaaattg gcgcgacaat
     4861 caccgtcatc gaccacaacg ccagcgcgat tgagcgtttg gctggcgggt aattattcag
     4921 caaaagactt tgcgaaagtg ggatcaacgg cccagcgaca atcccctgaa tcacacggaa
     4981 gaagatcagc atattcaggc tgctggagac accacacgcc cacgacgcaa tagcaaaggc
     5041 gatggtggac caaaggaaca gtttcacttc cccgacgcgc tttgccagcc agccggtaag
     5101 cgggatcgag atggcattcg ccaccccgaa agaagtgatt acccacgtac cctggctgag
     5161 cgatgagccc agattcccgg cgatagtggg gatcgccacg ttagcaatgg tggagtccag
     5221 cacctgcatg aatgtcgcca gtgacagcgc aatcgtcata atgaccagtt gcgcgccttc
     5281 cagcggtttt tgctgttgca tcacacgcac ctctggatta gccagcgtta gcttttacga
     5341 tatcgtcgat cagtttattg acaggtgcca ggctgatttc acgcgcggta cttaccgcga
     5401 ccggtgttga acgtacttta tttgccagta cctgaccgtc acggttagtg gtattgacgc
     5461 tcaccagcgt ggacaaacca atgcgcagcg ggtattgctc cagctgtttc tggtccagtt
     5521 cgatacgcac aggtagacgc tgaacgactt tgatccagtt accggtcgca ttttgcgccg
     5581 gaagcagtga gaatgcgcta cctgtgccca tatccagacc aaccacttta ccggtgtatt
     5641 tcacatcatc gccgtaaata tccgtggtga tagtgaccgg ctgaccgata cgcatattgg
     5701 caatctgcgt ctctttaaag ttggcagcca cccacatatt ggtggctgga acgaccgcca
     5761 tcagcggcgt cgttgggcta atttgcgccc caggctgtac cgcgcggcgg gagacataac
     5821 cggtcatcgg actgacaata cgggtacgct ccagcgccag ccaggcgtta cgtacttcgg
     5881 tggcagcctg ttgcacggca ggctgatctt ccagtttagt ccccagaatc atcgcctgat
     5941 tggcattgta ttgttgaata gcgacgtcca gttgcgcctg ggcactggtg acggcgtcgc
     6001 gggcgtgttg cagctcttcg cgaccaatca ggttggcatt gcccagcggc acacggcggt
     6061 tgtagtcgct ttgtgctttc gcaagggcga ttttttgcac ctcaatattc gcctgtaact
     6121 gtttgctgtt aatcatcagc tggtgggttt ggcgaacgct ggaagccagt gcagttttgg
     6181 ctttttcaaa cgcctggcga gcatctgtcg ggtcgagagt gaccagaaca tcgccttctt
     6241 ttacaaaatc ggtgttatcg gcccagactt tcgttacgct gccagacacc tgagacataa
     6301 tttgaatctg attccctgcc acgtatgcgt catcggtttc ttcgaaatga cgcagtacca
     6361 aaaaccaata aatccctatc gctacggcaa taattataaa gagcaaggtg agaaggagga
     6421 gcagacgctt acgtttgccg ctcttcttta ccggttgctg cggggtttga gtctccgcat
     6481 ttgcgctcat attgttctcc acgatcttct taactcatcg gctgagccga cctgcttgcc
     6541 aaaaaggcca gcatgttgcg atgctggccg gtcatttttt cttttataaa tctggatttt
     6601 tgagcgagat gacgcgttag ctcatcgctt cgagaaccac accgtcttgt tccatctggt
     6661 cgagacggga gagcaatttg cgggtgattt gctcgagctg atctttttct gttgtgctga
     6721 gcgcggacca gagttgatgc aggcagttat gctgcggtgg taaaacctcg cgcaaaaact
     6781 cgtgaccttt ttccgttaat tgcagatgca ggcagcggcg atcgttatcg ctttcacgac
     6841 gttcgatcca accgcgtttt tccagttcat cggcaatacg cgtcgcgttg gtacgggatg
     6901 atccaagagc acaacttaat tcagaaggct gaatactgtg gttttcctga gactccagcg
     6961 taatcaacgc cataaacaac gtctcgttaa tcccctgagc cttcagcatt ttattgcggt
     7021 tctccagcag cttgctttgc atgtgcatgc aaagacgagt cagaaggatc tcctgataag
     7081 gaaaatcttc gtggcggctg gcgcgaaatt ttagcatttg ttcaatgggc gtaaacgaac
     7141 tatccatttg ggtatgacct cattaatttc agccgatata gtaacgacag tgacaaataa
     7201 agtaaatgta ttgttttaga aaaatgattc ttgtgggtta tataatcgcc atcactttcc
     7261 aggcgagccc ataggccagc gcactaagca gtgttgggat gataatgctg cgtgttttat
     7321 agaaactggc acccagtacc gcgaagccga ccagcgtggg cacgaaacgg cgtgtatcgt
     7381 gcatcacttc tggtgcggta gagacaacca gcagagcgca tatcgaggcg atgccaatgg
     7441 tgtcgagcaa aatacctacc gcgccacgtt tggttgggcg ggcattaccc acacgcaggc
     7501 gcagcggcaa atagcggaag caataattcg ccacgccaac cagtaaccca agcagcagaa
     7561 cctcatagct catcgggcgc tccttgccag aatgcctgga ttaacgcagt gaggcagcca
     7621 cagacaatgc ctgccagaat ggcgacggga atagaaaata gcgttacgcc tgcaagggca
     7681 ccaactaacg ctgcggtaac gcaaagagat tgtttgcgct ggaaagaggc gagcaggaaa
     7741 ctcataaaga gtgccggaag cataaaacct aatgcagctt caacggcggg ataaccttgc
     7801 agcaagccgc tgccggagaa tgcccctatt accgtaccaa atacccacga tgaccatgaa
     7861 ctgaaggcaa tgccgatcat ccagttctcg ctccagcggc gattattgcg taccagtttt
     7921 gcggttgcgg cggcaaaaac ctcatccgtc aggccaaatg cccacagggc ggttttcgat
     7981 ttttgcagac gctgaataat acggctacgc agtgacgggc catacaacac atagcgaaca
     8041 tccattgcca tgacggtcag tgcagcaacc cacaaactac tcccggctgc cagcatcgcg
     8101 gtaatgacga actggctcgc gcctgcataa atgatgcagg agaaaaaaac gctttcgaga
     8161 ggagagaatc ccagacgggt cgcattcaga ccgaacgcaa aggccaccgg aatataacta
     8221 ataacaatcg gtaaactgtc tttgcatcct tccatgaagg tcgccgaacc aagagcaggc
     8281 tgtggagtag ggctttccat agagtgtacg cttaacgatt gttaatatta taaatagact
     8341 gaatgaatat cttaacctta tcagactgat gggcttctta acacccttat aagtgtaaag
     8401 ccacgaaaac ggttgctgat tgcttaattt gcggcttcct gacgatgaaa acctcgccac
     8461 cagaccagta agttaaccag ggcaatcgtc gcgccagcca gacaaacgcc agcccaaccg
     8521 ccatgttgcc aggctgaggc tgaaattagc gaaccggcgg caccgccaat aaagtagctg
     8581 gtcatgtaac ctgcggtcag gcgattacgc gcatcagggt gtattcgata aattaccgtc
     8641 tggttagtga tatgcacgcc ctgtacggtg agatccagca ccaggatgcc gataatcagc
     8701 gccagtacgg aagtgtgacc aaaccagatc gccagccatg aaagtaatag cagcagcaga
     8761 ccgaaagttg tggtgtggtg cgatttaccc ttatcggcaa aaccgcccgc cggacgagcg
     8821 cccaacgctc cggcagctcc cgcaagtcca aacagaccaa tgacaccatc gctgtagtta
     8881 aaaggtggag cggcaagcaa aaaggccatt gaggtccaga gaatgctgaa gttggcaaag
     8941 gtcaggcagc ccagcaatgc gcgggtgcgc aggatcttat cgctgataaa catgctgaaa
     9001 acggaaccta acaactgtgg gtagttgagg tgggtttctg atttcatttg tggcagacca
     9061 cgccataatg ccagcgccat cagtgccatt aacaccgagg caacccaaaa gacggtgcgc
     9121 cagccgccga gattcgccag caatccggca acggtccgtg cgagtaagat ccccaacagc
     9181 agaccgctca taatagtgcc aaccactttg ccgcgtttgt ccggtgaagc cagcgtcgct
     9241 gccagcggaa ccagaatttg tgccacgaca gagaataaac cggttaatgc cgtaccgagg
     9301 atcatcatcg ccagcgactg actgctggca gtgattaaca tgccgcctgc cgccagtaag
     9361 gtcatcgaga caatcaggcg gcggcgttca aacatatcac cgaggggaac aagaaacagc
     9421 agacctgctg catagcccaa ttgcgcggcg gtaacaataa agcctgccga actggcggaa
     9481 agggaaaagt tacgcgcgat ggtgtcgagc aatggctggg cgtaatagtt actggctacc
     9541 gccagaccgg tggcgataga catcagcacg atcagcgccg ggctaagctc atgattaggt
     9601 ttagtcatta tttttgttca tcaggtgatg attaagctgg aaatcaataa tagcggatgt
     9661 tgatgaatat gggatgaaag atagcttgtt gaaatgtgcg atgtggtttg taggcatgat
     9721 aagacgcgcc agcgtcgcat caggcattgt gcacagaacg gcggatgcgg cgaaaacgcc
     9781 ttatccgcca tacggaatga gtgtattttg taggcatgat aagacgcgtc agcgtcgcat
     9841 caggcattgt gtacagagcg gcggatgagg caaaaacgcc ttatccgcct gaataaaaat
     9901 tacttctgcg ctgccagcgc ctcattcacc cagccatcga actgctgctg gtgggctttg
     9961 atccagccat caacgtggcc ctgaatatcg ccttctgagg ctttgccgtc atgcataatg
    10021 gcgttctggg cgttaatatc ggccactggc aactgcataa tggcaaacag tttggctgcc
    10081 gccggatttt tctcggtcca ggctttgttg gcaacgatat gcatggtgct gaccgggaag
    10141 ccgtaattcg cgccattcgg cagtttggta tcggcatttt tatcgcccgg cagtgcggag
    10201 aacggcacct gcaaccagac cacatctttc cctggcttca gctcattact cacccagtac
    10261 ggcgtccagg tgtaataaaa caccggtttg ccctctttgt agcgactgat ggtgtcggcc
    10321 atcatcgctg cgtagttccc ctgattatgc gtcacggtgt tggtcagttc atacgcggca
    10381 agctggtggt tgatcgcacc ttcgcagccc cagccagggt tacaaccggt taaatccgct
    10441 ttaccgtcgc cgttggtatc gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg
    10501 ttggtgattt tgtactggtc ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg
    10561 ttaacaaata ccccttcacg ataaaatttc ttatcgccac cggcagcttc gtacatgttg
    10621 tcatgcagtg gcgtccagtt cacggcggtg aaggttgcat cgccggaagc aagcgaggtg
    10681 tagccaacgt tgtaatctac ttcgctgggt ttgttgacgg tatagcctaa tttctccagc
    10741 gcacggctga ccagcagcgt ctggaaggtt tcttcggtga tggtgctttg aactggatta
    10801 acagtaatac ctttgcccgg cagatcggca gcaaaagttt gtgtagagat aagcgtggca
    10861 aacgctgtcg caaaaagtac gctatgtcgc attgttattc ctttttttgg cagggtgata
    10921 atgcccggcg tcaccgggca agtgcagagt tacttaatga atgggcgggt cagcagacca
    10981 acagggccag tggtgtacca gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc
    11041 tgcgtcaggc gatcgaggat aatggcgagg atcacaatcc cgacgccgcc aacggtggca
    11101 agccccatat ccagacgacc gataccgcga agtaccatct gacccagtcc gccgacggca
    11161 atcatcgagg caatgaccac catagaaagg gccagcatca gcgtctggtt aacgcccgcc
    11221 ataatggtcg gcatcgccag cggtaactga actttgaaca gcatctggcg cgggctggca
    11281 ccgaatgagc gcgaggcttc aatcagatcc gccggaacct ggttaatccc cagaatggtc
    11341 agacggataa tcggcggcag agcaaagatg atcgtcacca ccacgcccgg cacgttaccg
    11401 ataccaaata gcatgacgat tggcaccaga taaacaaacg ctggcgtggt ctgcatggca
    11461 tcaagcagtg gacgaataat tttcgccgct cgcggacttc tcgccagcca gatccccagc
    11521 ggcaaaccaa tgacgataca gaacagcagg gtggttaaca ccagcgccag ggtaaccatg
    11581 gcctgcgacc aggcaccgat tgcgccgatg gcaatcagcg aaaccagcgt cgccacgccc
    11641 attccgaccc cggaaatctg ccaggcgatg agagcgaaaa cgataatcgc caccggtgcg
    11701 ggcataccca gcagcaattg ctggaaaccg ttgaggatat aatcaaccgg aacgcgcacg
    11761 ccctggaaga cgggacggaa atgggtaaca acccagtcga tcccttcagt gacccaactg
    11821 tcgagcggga tcagcgtttt atgaaacgga tcgagaatat taaaatgctc gacgtttggc
    11881 gcaggcgtac tggtcagcca gtcagcacca ccgccgtcag tcggtgcagt cgccggtgta
    11941 ccccaggcgt ctgcggattg cacggcactg tccgccgctg gcgtggtatc ccacggatta
    12001 ttttgatcag ccattattta ccccctcacg atctaaagcg cgcagcagca ttcctttcga
    12061 aatgatgccg acatactgtt ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc
    12121 gacatgagag agcaactcgc taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat
    12181 cagcgccgca tcaagacctt gctgctgcgt taacgcggtt ttaagcgaat cgatggaaac
    12241 tgcgccgaca aacttattac cgcgttcgat aacgtagcca tattcgcgat cttcatcctg
    12301 caataatttc agtgccgaac gtgggccgaa gccaggggtt ttacgaatta agccattcgg
    12361 tgtccggcgg gcaatatctt tcgcactgaa tacctgacta atatcaacgc cacggaagaa
    12421 ggtacggaca taatcattcg ccggattatt gagaatttca tccggtgtgc cgacctgtac
    12481 cacttcacca ttttgcataa tggcaattcg gtcgccaata cgcatagctt catcgagatc
    12541 gtgggaaata aagacaatgg tgcgctgatg tttcgcctgt aattttacca gctcatcctg
    12601 catctcggtg cgaattaatg gatcgagcgc cgagaaggct tcgtccatta ataatatatc
    12661 cggattaatc gctaacgcgc gggctaatcc cacacgttga cgcatcccgc cagagagttc
    12721 atccgggtag ctgtgggcat aattttccag cccgacctga cgcagtgcat caagggcttt
    12781 ttcccggcgt tcttcggcat taattccggc caattccata ccgaacgcag tattgtccag
    12841 cacggtcata tgcggcatta aggcaaagga ctggaagacc atcgcaatct tttttctgcg
    12901 cacctcacgg agttcggcat cggatatttt ggcaatatcc acaccatcaa tcagcacttg
    12961 cccgcgggtg ggttcaatca ggcgattgag aaggcgtacc attgtggatt tacccgagcc
    13021 ggataatccc atgatgacaa atatctcgcc ttcttcaatg gccagactgg cgtcttttac
    13081 gccaagcgat agcccagttt tttccagaat ttgttctttc gaaagtcctt gttcgatata
    13141 tttgaacgct cgctgtggat gctcgccaaa tattttataa agatttttaa tttctaattt
    13201 aattgccatg caatagaaag attcctttat ttgtttatgt cgatatatta cccaatggaa
    13261 atagtcactt ttttctaccc taacatactg agaatctgag gcaacccctg agggcaaatg
    13321 tggcatgaat attgatgcga gcaaatcgcc ggaatttccc gtgatataag ggctgagagc
    13381 aaatcgagaa aagttgatat tttttgtcat agatagcgaa aaaacgcctg aatattgtag
    13441 tttcaggcga ataatgcaac agagtctcag tgtagatcac tacagaatat ttgcgtgata
    13501 aaaagctatt tggcgggaat tatttccctg ctgcgggtag tgatattttt gaaaataaca
    13561 ccctcagaaa ttccagtctt catcttctgt ttcaaccgct ttccccatca cataagagga
    13621 gcctgaaccg gaaaagaaat cgtgattttc atcggcattc ggcgaaagcg cggcgaggat
    13681 tgccggattc acttccgcca tttctgcggg aaataacggt tcgtagccca gattcatcaa
    13741 agccttattg gcgttgtaac agagaaacgc tttcacatcg tcagcccacg gggtttcggc
    13801 gtacagctca tcggtgtatt gcaactcgtt gtcgtagagt tccagcaaca aatcgaaggc
    13861 gaaattcttc aactcttcac gttgtcccag agatatcttt tccatgtttt tctgatattt
    13921 atagcctatg tagtaaccgt ggactgcttc atcgcggata atcagacgga tcagatctgc
    13981 ggtattggtc agctttccgc ggctggaaaa atacatcggc agccagaaac cggaatagaa
    14041 caaaaaagat tcaagaaaca cactggcgat tttctttttc agcggatcat caccgcgata
    14101 atgttgctga ataatctgag cttttcgctg caacggtgcg ttttcttcac tccaggcgta
    14161 ggcggcatcg acatctttgg tctgacacag cgtcgagaaa atcgaactgt aagagcgggc
    14221 atgaaccgct tccataaagc tgatattcga taataccgct tcttcatgag gcgtgagtgc
    14281 atcgggcatc agcgaaggcg cgccgataac attttgcagc gtgtcgagca gcgtcaggcc
    14341 agtaaaaacg cgcatcgtca gttgttgttc tacgacagtt aatgtctgcc aggcaggaat
    14401 atcgttcgac agcggcacct tttctggtag ccagaaattg ctggtcaggc gattccacac
    14461 ctccagatct ttatcgtcag atatcttgtt ccagttgatg gcgctgatac gtgagagttt
    14521 catagatatt ccttaaagtg cacaggagac gcagccttca atttcagtgc cttccagcgc
    14581 catctgacgc aggcgaatgt aatagagcgt tttgataccc ttgcgccagg cgtaaatctg
    14641 cgctttgttg atatcgcgag tggtggcggt atcggggaaa aacagcgtca gcgacagccc
    14701 ctgatcgaca tggcgagtcg cttcggcgta ggtatcgatg atcttttctg cgccgatttc
    14761 gtaagcatcc tgatacagcg ccagattctc gttagtcata aacggggcag ggtagtaaac
    14821 gcgtcctgtt ttgccctctt tgcgtatctc tactttcgcc acaatcggat gaatactcga
    14881 cgtagcatgg ttgatataag agatagaacc ggttggcggc accgcctgaa gattctggtt
    14941 ataaatgccg tagcgcatca cgtcgtcgcg cagttgcgcc cacatctcac gggtaggtaa
    15001 cgtaataccg ctgcgggcaa acagttcgcc aactttcgcc gttttcggct gccagttacc
    15061 ttgcagatat tggctaaaat attcaccact ggcataacgt gactgtttga acccggcgaa
    15121 ggtttcaccg cgttcgcgcg ccagcaacat cgaggtacgc agtgcgtgcc aggtgatggc
    15181 atagaaatag agattggtga aatccagtgc ttccggcgaa ccataagcga tgccttctcg
    15241 cgccagatag ccgtgtaaat tcatctgccc cagtccgatg gcgtgtgagg cggcatttcc
    15301 agcttcgatg gacggcacgc tgcggatatg actcatatct gacaccgccg ttaaaccgcg
    15361 cacggcagtc tctaccgtgc gggcaaaatc gggggaatcc atggtgtgcg caatattcaa
    15421 cgaacctaaa ttgcaggaga tatcatggcc tgtgcgggca tagtcgagat tctcgtcata
    15481 ctctgaggca cagttgacct gcaaaatctc tgagcagaga ttactcatat ttatgcgccc
    15541 ggcgacaggg ttagcacggt ttaccgtgtc ttcatacatg atgtagggat agccggactc
    15601 aaactggatt tctgccagcc gctggaagaa atcacgggcg ttgaggtatt ttttgcggat
    15661 gcgttcatcg gcaaccagtt cgtcatagtg ttcgctgatg gcgatatcgg caaacggctt
    15721 gccataaact cgctcaacgt catagggcga aaacagcgcc atctgcgcat tctcttttgc
    15781 cagatggaaa gtgatatccg ggatcaccac gcccagcgac agtgttttaa tgcggatttt
    15841 ttcgtcggca ttttcccgtt ttgtatcgag aaaacgcaga atatcgggat gatgagcatg
    15901 taaatagact gcacctgccc cctgacgcgc gccgagttgg ttggcatagg aaaatgcgtc
    15961 ttccagcatt ttcatcaccg gaattacgcc agaagattga ttttcaatac gtttaattgg
    16021 cgcgcccgct tctcgcagat tcgacagcaa aaatgccacg ccgccgccgc gtttcgacag
    16081 ttgcagtgcg gaatttaccg cccggccaat cgactccata ttgtcttcaa tacgcagcag
    16141 aaaacaggaa accagttcgc cgcgctgctg cttaccgcag ttgaggaatg tcggcgtggc
    16201 tggctgaaag cgtcctgaca gcatttcatc ggtcagttgc aacgccagcg tcgcatcgcc
    16261 ttgtgccagc gtcagcgcca ccatcgtgac tcgatcggca aaatcttcca gataacgttt
    16321 accgtcgaat gtcttcaacg tatagctggt gtaaaacttc catgccccga ggaatgtctg
    16381 gaaacgaaaa ccgctggtgt gtgcgtgggc aaacagcgta atgacaaagt cgcgagagta
    16441 gcgattaaga acgctttcat catagtaacc ttcgttgacc agccagttca ggcgctgctg
    16501 ctggctactg aaggtcacac tgttcggacg cacatgcgtc gccataaagg cgtcaacggc
    16561 ctggcggtct ttatcgaact gaatgcgacc tgcgctatcg tagaggttaa gcatcgcatt
    16621 cagcgcgtgg taatccatcg tttcctgcgt caggcattct gcggttgtcg ttgccaaaat
    16681 tcggttactc ctttacgaac gttttcgata tcgctttgcg tacccatgag ttcaaaacgg
    16741 tacagccacg gcacgccgca tttccgggca atcacatctc cggcgcggcc atacgcctca
    16801 ctaaagttgc gattaccaga agcaataacg ccgcgaagca acgcccggtt gtgctcgtcg
    16861 tttaaaaagc gaattacctg tcgtggcacc gcgccagccg taccgccgcc gccgtaagag
    16921 ggcacgatca ggatgtaagg ctcgtctacc tgaatccgtt cccgctcatt gagcgggatg
    16981 cgcaccgcgg gcagacctaa acgttcgata aaacgctgcg tgttttcgga gctgctggag
    17041 aagtagacga gctggctcat gcactggccg cgtgtggcgc tggatgcaga cggttaatca
    17101 tgtccggacg gaaaccagac cagctaagat cgccagcaat cactaccggc aactgacgaa
    17161 agccctgagc acgcaacgct tccgctgctt caggaacgcg atcgacatta atcatttcaa
    17221 aatcaaaccc ccggttttcc atcgcccgtt tggtggcgtg gcactgaacg caatcgttac
    17281 gagtgtaaat agttatgcgc atgattcgta tttccgttta aaatgaagat acggcgcgat
    17341 gatacgcgtc gggttgtctc tctgttgata cagagatact agatgtagtt gaaaaaagat
    17401 tcaactacac aatatatagc aaatagtgat cgaaattacc ctggatatga gcgtgacggg
    17461 ggagggggat ttttgtgatt caccaggcaa aaagaaaccc cgaagacagg cttcggggtc
    17521 aaagacgcgt atttattatc atttttgcac tacgatttgc gcatgcttaa cagtgcgccg
    17581 ataaaaatac ctaccgcagc tgctgtaccc acgctacacc agggtctttc acgaacaaaa
    17641 gaatccgcgc agccaacggc atcgcgcgcg gcttgctgga cgcgagtacg accatgcatt
    17701 cgcgccctgg tttctttcat caacgcctga gctttactgc gtgcggcttc agcttcccct
    17761 ttggcgtcgc tgccccagga cttcaataca gattcgaggc tgtcagctaa ttgattgaca
    17821 tcattctgga tatcctgcac gccatcatca acgtcgttgc ggttcggtcg gttaaacata
    17881 tgatcaccca ttagaattcg atgtaatttt cagtgtagac cataattttc ttaactgagg
    17941 ttcatcgcgg ctttatggac ttttctgaaa gcgttgtgaa tgcgcaatca ggtaaggtag
    18001 gcccgcgg
//
LOCUS       DAJTNA010000078        17546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTNA010000078 DAJTNA010000000
VERSION     DAJTNA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17546
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.079"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..342)
                     /locus_tag="OO161_002199"
     CDS             complement(<1..342)
                     /locus_tag="OO161_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5463254.1"
                     /translation="MITIETLYGECHEAHGLKIRNQVNNLFQKHPRITAIRVDLHYPP
                     VIDNGDTITCFPNLSPGEISRFRNSLNAKINADQHRKKQQGKRTYATSVCIIWAKEYT
                     QSFKCHYHVCLF"
     gene            complement(724..1224)
                     /locus_tag="OO161_002200"
     CDS             complement(724..1224)
                     /locus_tag="OO161_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5463255.1"
                     /translation="MIQRYFKEPLLQLTGELGRPDYTLADRKRLVRLLTQDMQRGDIL
                     ITKSLTCFGNTPEKIQEVLSLSIRQGINICFFTSSELPDGDDIPVVLTGMILPTDIRG
                     KIPDISIRKIKRKPGRKEGSIHSKNIRQLKLQGFSQSQTARKLSISLSTVKRHWDKGI
                     VDLTAE"
     gene            complement(1506..2252)
                     /locus_tag="OO161_002201"
     CDS             complement(1506..2252)
                     /locus_tag="OO161_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463256.1"
                     /translation="MMKRVSFSLAETYEADVIKKYQHLKKCSFSAAIKECLKLGAPVL
                     NRINENIAAITDIEDKLRQFFNEEPFVQRTRPEITKGEFFHSIYKSHIKYEYDVLDRK
                     IFPHESTRNAMGVAEKKGIKENATLMLEYYKVEKAICIYTNRKVSHTLNRAGGFYKTI
                     LIKTSVFGDYFFDFCNSVCLPIDELIEYGTKETVRRHQIRSTGFCTFHIPIFYINNKA
                     VIVPVLRTEEVSQSSRTGGDVIIINPFEDE"
     gene            complement(2315..2542)
                     /locus_tag="OO161_002202"
     CDS             complement(2315..2542)
                     /locus_tag="OO161_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR5463257.1"
                     /translation="MWKPLRDENRLSILKIYGYTCDRLVREKERQKITSISRSQAWKL
                     EREGKFPPRKTIGKKSCGWLLSDLLWWISQY"
     gene            3323..4927
                     /locus_tag="OO161_002203"
     CDS             3323..4927
                     /locus_tag="OO161_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463258.1"
                     /translation="MVTKKVKTNPPYYRVLSGTSMPFPHIHEAVSEQLLLLIFKARRL
                     YLDPEYRPWFDEQHRILSALRANTKLQSILEKNRENYSISETDNPLSKDLYAAFQVMC
                     HLYNGFDNRLVSLKTWGDMNREPTAKFVGQNSGGWKTPEMEEFVRNDLFNILTDCKAH
                     CSPLPALVRQGYCHQDLSVAHSCTKELLEQLNRYHQIDESAILARTLGLAINNCYHSE
                     TEIPEWLSPFENEYKSPWNCEKPDTNDVSKLRKQLLTIGRTEFVDKMFSLANKNATKI
                     IYDGYVKLYALEIYEWLNKLVQYFKGNDFIRYTWKFTTIISDESDTIENTTAWEKDYC
                     QITIQRDELLKLIYTNTLQDAAMNLADCLQQAYTEPLKMEINYPGINIDAKERKIRKS
                     IEFRSELNNFTNIMKQLEIFEKWYNANKILLVSNKKIRTEKIDVNIRLAGLKGYDLNE
                     GIPDGQGRRIKDGVNELVKADGSLQLPQDISDTSLNRYRQAVQHIIKDEIDTLLIAQR
                     EKNKINPYSEDRYSIRPLWGKCLVEQ"
     gene            complement(5010..5606)
                     /locus_tag="OO161_002204"
     CDS             complement(5010..5606)
                     /locus_tag="OO161_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463259.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDVRHKVVATLELQRRPRLEGVFVQKFWEEQDVAQIEGIVVDGAVRDVGL
                     ATFVYETIVTKAGVVLLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGE
                     RISYDGKSIPESEIWSEHPERNRHAVVLVAESVNGKAA"
     gene            complement(5736..7052)
                     /locus_tag="OO161_002205"
     CDS             complement(5736..7052)
                     /locus_tag="OO161_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5463260.1"
                     /translation="MAVNLTETAIRALKAKKTSFYVWSNSSQRGTGRLGVKVQPSGSK
                     IFYFRYYVEKGKKERFIQLGIWPEMKLATANELAKKYGAWLIEGKDPQAELERQQFAE
                     QQRIQLHQSQGSFEALIHGYVNKMKIDNKRTWQDVLKRLENECYTVIPRETKAKDVTS
                     GQIKHILAGIIQRGAVVHSNRIRSYLMAAFNYGLKADNDPMNTSVGITFGLEANPVSV
                     IPKQSSAEKVGDTWLTLEELRFLMEHFAEATNVGLLMQHLIRFCIYTGGQRPFEMIAS
                     QWCDVDFQQKTLLVTADVSKNKREHLIPLTESALQELSCVQELTKEKSSPYIFPLSTN
                     GKRPVRTDSLARAIMYFRAFNPDFKIFTARDLRRTCKTLMGEAGISKEIRDRIQNHAL
                     NDVSSKHYDRYDYLPEKRRALEIWEDRVNNYQQQTGNNVVNLFGRR"
     gene            complement(7349..8113)
                     /gene="nfeF"
                     /locus_tag="OO161_002206"
     CDS             complement(7349..8113)
                     /gene="nfeF"
                     /locus_tag="OO161_002206"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR5463261.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRSFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            8401..9024
                     /gene="nfeR"
                     /locus_tag="OO161_002207"
                     /gene_synonym="yqjI"
     CDS             8401..9024
                     /gene="nfeR"
                     /locus_tag="OO161_002207"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5463262.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(9178..10698)
                     /gene="aer"
                     /locus_tag="OO161_002208"
     CDS             complement(9178..10698)
                     /gene="aer"
                     /locus_tag="OO161_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR5463263.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQGLQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLEVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            11115..12494
                     /gene="ygjG"
                     /locus_tag="OO161_002209"
     CDS             11115..12494
                     /gene="ygjG"
                     /locus_tag="OO161_002209"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5463264.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVVAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(12536..12868)
                     /locus_tag="OO161_002210"
     CDS             complement(12536..12868)
                     /locus_tag="OO161_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR5463265.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            13087..14070
                     /gene="ebgR"
                     /locus_tag="OO161_002211"
     CDS             13087..14070
                     /gene="ebgR"
                     /locus_tag="OO161_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5463266.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            14254..17346
                     /gene="ebgA"
                     /locus_tag="OO161_002212"
     CDS             14254..17346
                     /gene="ebgA"
                     /locus_tag="OO161_002212"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5463267.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYQHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            17343..>17546
                     /locus_tag="OO161_002213"
     CDS             17343..>17546
                     /locus_tag="OO161_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5463268.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEV"
BASE COUNT         4800 a         4333 c         3925 g         4488 t
ORIGIN      
        1 aaaaaggcag acgtgataat gacatttaaa ggactgcgta tattctttcg cccagataat
       61 acaaaccgac gttgcgtagg tgcgtttgcc ttgttgtttt ttacggtgtt gatctgcatt
      121 aattttcgca ttcagcgagt tacggaaacg agatatctct ccgggactca ggttaggaaa
      181 acaggtaatg gtatccccgt tgtcaatcac gggaggataa tggagatcca cccgtattgc
      241 cgttattctg ggatgtttct ggaagaggtt attcacctgg tttcttattt ttaacccgtg
      301 agcctcatga cattcaccgt aaagagtttc aatcgtaatc atgttaatat tccttttgct
      361 ttcagagata agctaatagt taacctaccc aatacacaac agaagcagat attaatattc
      421 gtatcagtag ttcccagagg gatatataac cctctgggcg tcaattaaga aaaacgctac
      481 tgcctattgt tacattagag aacaggatga tgttacacag ttacattaga acgttctaaa
      541 acattaaaca gagttatgat ctacacacaa ctaattattt ggtatcagaa taccattagc
      601 tctattagta catcatatcc atcattacac atatactata catcacagat ataatatata
      661 ttacctatcc aacgcacatg tatttataac tccattgcca atagctacaa ggactataaa
      721 atgttactct gcagttagat caacaatacc tttatcccag tgtcttttta cagtggataa
      781 actgatgctt aattttctgg ctgtttgtga ctgactgaac ccctgcaatt tcaactgtct
      841 gatattttta ctatgaatac tcccttcttt ccttcctggt tttcttttaa ttttccggat
      901 actgatgtcg ggaatttttc ctctaatatc tgtaggtaaa atcatcccgg tgagtaccac
      961 aggaatatca tcaccatccg gcaactctga tgacgtaaaa aagcaaatat taataccttg
     1021 tctgatactt aaagataaaa cctcctgtat tttttccggc gtattaccaa aacaagttaa
     1081 cgatttagta atcagaatat cacccctttg catatcctgc gtcaggagtc gaactaaccg
     1141 ttttctgtct gccagagtat aatccgggcg acccaattcg cctgttaact gcaatagtgg
     1201 ttctttaaaa tatctctgaa tcatggattt taagcgatta agttttgacc atttttcaga
     1261 gtctatgtat gcaaaactcg ccataatgcc tcaaactggt gtcatgaatt aattattgaa
     1321 cctgtttttt cctgagtcgc attactcttt tctaagggag gctctccaga tacatcggaa
     1381 aagtactcga taaaagagtt ggccactccc gggtaatgat tgctctcttt aacctcagaa
     1441 agcggtttct gttcatcatt tacaccacga tttctgggtt atgaataaat ctacgcgggc
     1501 cataatcatt catcttcaaa tgggttaata ataataacat ctccacctgt tctggaactt
     1561 tgtgaaactt cctctgttct cagaacaggc actattacag ccttattatt gatataaaaa
     1621 atcggaatat gaaaagtaca aaatcctgta ctacgaatct gatgtcgcct cactgtttct
     1681 ttcgtaccat actcaatcaa ctcatctatc ggcaggcaaa ctgaattgca aaagtcaaaa
     1741 aaataatccc caaaaacact ggttttaatc agaatggttt tataaaaccc tcctgcacga
     1801 ttcagggtat gggaaacttt tctgttcgtg taaatacaaa ttgccttctc aactttataa
     1861 tattccagca ttaaggtcgc attctcttta attccctttt tctctgcaac tcccatagca
     1921 tttctggtgc tttcatgagg aaatatcttt ctatcgagaa catcatattc atatttgata
     1981 tgcgatttat aaatgctatg aaaaaactct ccttttgtta tttcaggcct tgtccgttgc
     2041 acaaatggtt cctcattaaa aaactgacgt agcttatcct ctatatcagt tatagcagca
     2101 atattttcat ttatcctgtt taaaacagga gcccctagct ttaaacactc tttaatcgct
     2161 gctgaaaatg agcatttttt taaatgctga tactttttaa ttacatcagc ttcatatgtt
     2221 tctgcaagag agaaagagac acgtttcatc atgaaactcc tttttaatta taaaattaaa
     2281 atatagaaat atatattatt ctgatactat aaggttaata ctgactaatc caccacaata
     2341 gatcacttaa taaccaccca caggatttct ttccaatagt tttacgtggc gggaattttc
     2401 cttcacgttc cagtttccag gcttgtgaac gagaaataga cgtaattttc tggcgttcct
     2461 tttctcgtac cagtctgtca caggtgtatc catagatctt caaaattgat aaacggttct
     2521 catctcttaa cggcttccac atattgctct ccttatatat tcatgatttt cagattgtat
     2581 atgcatgtga gctgctcaaa tcactacatc tatctgtagt ggtatgctgt tgaacagcac
     2641 cagcaactca cgagcaaaac aatacatttg cacaaccaag aaatcaaatt gcgagaaatg
     2701 atttaattaa ctgacttcta ataacacaat aacaacaaag aaagtcacaa tataaaaata
     2761 actcaaacaa tgctttttgg catacaaaat aaaaatcaaa atcacaatat tcgtaggtta
     2821 ttttatgcag acaaaaacaa aaaaaaatct tgttataatc tatgatatca agaggagggg
     2881 tagtgaccaa aaattctccc cagaaaagcc cccacaacat tagtttgaaa acgtgacgaa
     2941 gttcacaaaa ttatcttctg gcgataatag aacttgtaag agggagttga tttttgccaa
     3001 ctttctaaag ctaaagtgca gacaaaagat aataattgaa tgaaaacgat acatattttc
     3061 agatgaaagt gacgtgactg tagagggcta taaccgtaaa atcatttgcg tcctgagcaa
     3121 tatgcaacaa aagggaatgc tcgtaaaaaa atcaggaata aacgctaagg atatcgtatt
     3181 agattcagca tcgtataatg ccactgcata actgattccc atttcgtaga ggctgttaac
     3241 tctgtatcaa tgtcctgaca aaggactgta tcaagtttct ctactggaaa cagctatgca
     3301 atagacggaa gaatttaaca ttatggtaac taaaaaggta aaaacaaacc ccccgtacta
     3361 tcgtgtttta tccggcacct ccatgccgtt tccccacata catgaagctg tttccgaaca
     3421 gttgcttcta ctcattttca aagcacgcag attgtatctg gatccagaat atcgtccttg
     3481 gtttgatgaa caacatcgga ttctctcggc attacgcgct aacacaaaac tccaaagtat
     3541 tttagagaaa aacagagaga actactccat aagtgagacc gataatccac tatctaaaga
     3601 tctgtatgct gcttttcagg ttatgtgcca tttatacaat ggttttgata acagactcgt
     3661 cagcctgaaa acctggggag atatgaacag ggaaccaaca gcaaaatttg tcggccaaaa
     3721 tagtggtggc tggaaaacac ctgagatgga agaattcgtc agaaacgatt tgttcaatat
     3781 tctgacagat tgtaaagctc attgctcacc acttcccgct ctggttcgac aaggatattg
     3841 ccatcaggat ctttctgttg cgcactcatg tactaaagaa ttactggaac aactgaaccg
     3901 ataccatcaa attgatgaat cagcgatact tgccagaacc cttggtttgg ctattaacaa
     3961 ctgctatcac tcagaaacag agattcctga atggctttct ccttttgaaa atgaatacaa
     4021 aagtccctgg aattgtgaga aaccagatac taacgatgtg agtaaactta gaaaacaatt
     4081 gctaacaatc gggcgtacag aattcgtgga caaaatgttt tcgctagcca acaaaaatgc
     4141 aacaaagata atatatgatg gatacgttaa actttatgct ctggaaatat atgaatggtt
     4201 aaataaatta gtgcaatatt ttaaaggaaa tgattttatt cgttacacct ggaagttcac
     4261 aacaattatt tccgatgaaa gtgacacgat tgaaaacacc accgcatggg aaaaagacta
     4321 ttgtcaaatc actatacagc gtgatgaact tctgaaactg atttatacca ataccctgca
     4381 ggatgcagca atgaatcttg ctgattgcct gcaacaggca tacacagaac cgttaaaaat
     4441 ggaaataaat tatccaggaa taaacattga tgcaaaagag cgtaaaatta gaaaatctat
     4501 tgaattcagg agcgaactga ataacttcac aaacatcatg aaacaactgg aaatatttga
     4561 aaaatggtat aacgccaata aaattctgtt ggtaagtaac aaaaagatac gtacagaaaa
     4621 gattgacgtg aatattcgtc ttgcaggact gaaaggctat gatctgaatg aaggtatacc
     4681 tgatggtcag ggacgcagaa ttaaagatgg tgtgaatgaa cttgtcaagg cagatggcag
     4741 tctacaactt ccacaagata tcagtgacac ttcgcttaac cgttaccgtc aagcagttca
     4801 acacattatt aaggatgaga tcgacacgtt gctgatagca cagagggaaa aaaataaaat
     4861 aaatccatat agcgaagata gatactcgat acgaccttta tggggaaaat gtctggtcga
     4921 acagtaactc actgagaaag gggatagcag ttcgctatcc ccaaaggaga ctttccagca
     4981 agatctcaaa aaatgagaca ctcagcaaat tatgcggctt taccattaac agattcagcc
     5041 acaagaacga cagcgtgtct gtttcgctca ggatgttcac tccagatttc tgattccggg
     5101 atgcttttcc cgtcataact gatccgttcg ccatcaaacg ggtaataccg tgcggaatca
     5161 gtatctaaaa taaacacctt cagattcgtt gaacgacgcg caatatgctg ccagagagct
     5221 ttacctcctt catattgctc gttatcactt aatagtacaa cgcccgcttt tgtgactatc
     5281 gtttcataaa caaacgttgc aagaccaaca tcacgtacag cgccatcgac aacaatccct
     5341 tcaatctgag caacatcctg ttcttcccag aatttctgga caaacacgcc ttcaagccgg
     5401 ggacgacgct gaagctccag agtggcaaca accttatggc gaacatcacc atcaataaca
     5461 gaaggtgcag cttcgattac cgcagcaaac ggagtctttg gatcccagat gatctggtaa
     5521 agcacgccct gaatagttac tccatccagt atcgaaaaac gacgagctac agtagcaggg
     5581 gagtaagaca ttggaattac agccattttt gaatcccctc tgagtaatac cggcacgaaa
     5641 atttacctca ataattatag taaacaatac aagaaccaag tgcatcaaca atacccatca
     5701 attgtacaca attcgtacag caaccaggaa ctccctcacc ttctgccaaa cagattgaca
     5761 acattattgc ctgtttgctg ttgatagtta ttaacccggt cctcccagat ctcaagtgct
     5821 ctgcgttttt ctggcaaata atcgtatcga tcataatgtt ttgaactgac atcgttcaac
     5881 gcatgatttt gaatacgatc gcggatttcc ttactgatcc cagcttctcc cattaacgtt
     5941 ttacaggtcc gacgtaagtc acgtgctgta aaaattttaa agtcaggatt aaaagcccgg
     6001 aaatacatga tagcgcgagc cagactgtcc gtacgaaccg gacgcttacc attagtcgaa
     6061 agtggaaaaa tgtaagggct ggatttttct tttgttaact cctgcacaca ggataattcc
     6121 tgtaatgctg attcagtcaa cggtatcaaa tgctctcgtt tatttttaga gacatcagca
     6181 gtcaccagca gcgttttttg ttgaaaatca acatcacacc actgactggc gatcatttcg
     6241 aaaggacgtt gccctccggt atagatacag aatcggatca aatgttgcat caataatcca
     6301 acgttggttg cttcagcaaa atgttccatt agaaaacgca actcttcaag tgtcagccag
     6361 gtatcaccga ctttttcagc agatgactgt ttcggtatca ctgacaccgg atttgcctcc
     6421 agcccaaaag taatccctac acttgtgttc atcggatcgt tatctgcttt cagtccatag
     6481 ttaaatgcag ccatcaggta agaacggatc cggttagagt gaaccacagc accgcgctga
     6541 atgatcccgg ccaggatatg tttaatctgt ccggaagtca catctttagc tttagtttca
     6601 cgggggatca ctgtatagca ttcattttca aggcgtttca gaacatcctg ccatgtcctc
     6661 ttattatcaa ttttcatttt gttaacataa ccatgaatca atgcttcaaa tgagccctgt
     6721 gattgatgga gttgtatccg ctgctgttct gcaaactgtt gtcgttccag ttctgcctgg
     6781 ggatcctttc cttcaatgag ccaggcacca tacttttttg ccagttcatt cgctgtggcc
     6841 agtttcatct caggccatat acccaattga atgaatcttt cttttttccc tttttccacg
     6901 taatagcgga aatagaaaat cttgctgcca gaaggctgaa ctttgacacc aagtcgtcca
     6961 gtgccacgtt gagagctgtt gctccagaca tagaacgatg tcttttttgc tttcaatgct
     7021 cggatggctg tttcagtaag attgacggcc atagaacctc ttttctaaac cagtaacaat
     7081 cagaataact cgtgattttg ggtcaggtaa tgggtcaggt aacgatcgta caatgaagaa
     7141 aaaacggaac caagcggaga cagggaaaat cacataattt attgattaca aacaactaat
     7201 aaagcaatca cgaacaaaca aaaacaatat agaacgaact tgcctcatga ctcataatcg
     7261 cttggtcgct ggttcaagtc cagcaggggc cactagatat agcaaaggct gacgagaaat
     7321 cgtcagcctt tttcttttta tatatcagtt actttgcgtg ccagtaagcc gctgcacgta
     7381 cccgctgtgg gtcatactgt tccgcttcaa agcggcggct taaattctta acgactttac
     7441 cttcgccggt tatccagatg aagtaatcat cggcagggat ttgcatctgc gccagacgcg
     7501 catctaccgc ctgctcatca tgtgccagcc attcgatatt aaaaccatca aggtgcgcga
     7561 gataatcctg acaggcgtta tcccgcacgc taaccagcgc actaacttgc ggtttaacgg
     7621 caagtttgct caacgtttcc aggcggcggc gcagtgcggg cattccggat tcatcgcaga
     7681 catacagctg atacgcgtaa tcttccggca ccaccagcga accgcgcgga ccagccaccg
     7741 taagtttatc gcccggttgc gcctgcatcg cccagccgct ggcgacccca ccgtcgtgaa
     7801 taaagaaatc gatcgccagt tcatggcgta gttcgtcata cagcggcgta tagtcacgcg
     7861 acggtgggcg tggtccttcc ggccagacga tgccctcttc cgttaccgtt ggcggcacaa
     7921 agtgagcgtc aggttgagga aagaagagtt tgctgtgatc gtcaaagctg cgcgatgtaa
     7981 aaccgtccag cgcctcgccg ccgaggacaa tgcgctgaaa accggcgctg atgcgctcaa
     8041 cgcgtaacac agtcagttca cggaagcgca gatcattgcg aacgcgctgc gggtagcggg
     8101 gggagttatt cattgttatc gccttcgtga tggtaatcag atatatctaa ataattttcg
     8161 taaatgataa tgattgttaa tcatgataaa tgcaagcgat tagtagaact gatatgtcta
     8221 tagtctgata agacgaaccg cctcttctca ggcatcatta ctcaacgccg gatgcggcgt
     8281 gaacgcctta tccggcctac gtgtgagatg agtccacatt taaaaatcat ttttattctt
     8341 gcaagagtgc tcatatcaga tataaattag atatatctaa ttaagcaaaa ggaggctgat
     8401 atgagccatc atcacgaagg gtgttgtaaa catgaaggcc agccacgcca tgagggctgc
     8461 tgcaaaggtg agaagtcaga acacgagcac tgcggacacg gtcaccagca tgaacacggt
     8521 caatgctgcg gtggtcgcca cggtcgcggc ggcggtcgtc ggcaacgttt ctttggtcac
     8581 ggtgaattgc gtctggtgat tctggatatt ctctcgcgcg atgacagcca cggttacgaa
     8641 ttgattaaag cgattgagaa tctaacccag gggaattaca ccccaagccc gggcgtcatc
     8701 tacccaacgc tggattttct gcaggagcag tcgctgatta ccatccgcga agaggaaggt
     8761 ggtaagaagc agattgcgct gaccgaacaa ggcgcgcagt ggctggaaga aaaccgcgaa
     8821 caggtggaga tgattgaaga acgcatcaaa gcgcgttgcg ttggcgcggc gctgcgccag
     8881 aacccgcaaa tgaagcgggc gctggataat tttaaagcgg tgctggattt acgcgtcaac
     8941 cagagcgata tcagtgatgc acaaataaaa aagatcattg cggtgatcga ccgcgccgct
     9001 tttgatatta cgcaactgga ttaatcgccg catccgccag tggcgcggtg caattgccgg
     9061 atgcgacgct tgacgcgcct tatccggcct acacccgcta cacaccccgc aggcctgata
     9121 agatgcgcca gcatcgcatc aggcattgtg ctccaaccgc cggatccggc ataccgatta
     9181 atgcagcacc gtcaccgcgt cttccagtcg gctggcgcgg tgtttcacca tcgccgacac
     9241 ctgcgcactc tcttccacca gctcagcatt tttctgggtg atcaggttaa gctcatccac
     9301 tgcacgggtc aggctggaaa gcccatcggc ctgttccagc gttgaatggc taatctgggc
     9361 gatcaactgg gtgacgtttt tcacctgtgc cacaatatct tccatcgtcc gtccggcggc
     9421 gtgtacctgc tgcgaaccgg attgcacctt atcagcactg gcatcaatca gcttgcgaat
     9481 atcgttggca gcattagcgc tgcggctggc taaatgacgc acttcccctg ccaccactgc
     9541 aaaacctttg ccctgttcgc cggcacgcgc cgcttccacc gccgcattca gggccagaat
     9601 attggtctga aacgcaatat cgttaatcag cgaagtaatg gtgccaatgc gctgggtact
     9661 gtcggcgata tcgtccattg tcttgatcac cgtggtcatc gcctccccac cctgcactgc
     9721 cgcattactg gcggtgatag aaagtttatc ggcagccgac gccgtggcag agttctgttt
     9781 caccgacgcc gccatttggt tcatggtcgc caccgtttgc tgaacgttat caactgtctg
     9841 ctgggtatgt tcgttcagtt catcggtgcc tttcgccagc gtctcactgc catttctgac
     9901 actggacacc tggcttgaga catcgttaat tagccaacgg cacatcaggc caagctgccc
     9961 taccgcgcgt aatgtcagtc ccagctcatc gctgcgattc aggtgctcaa cactattacg
    10021 ctctccggtc gccaccttca gtgcctgacg ggcgacattt tctatcgggc gcacaatctg
    10081 ccattcaaaa caagcgcttg ccaacaatac cactaacaca cagaggaaat acgtcaccac
    10141 cggggcagca acaaaccaaa gcatggccgc cagcaagata aacatcaggg tcatcactcc
    10201 acgcgcccgc cagcgaagcg gtaatgaagg cagtttaccc agccagcctt tacgcacctc
    10261 caggccttta tgaatacgct tactggtacg tccggcgttc agcgctttgt acagcggctc
    10321 caccgccgca atctcttcat ccgtcgcccg ggtacgaatc gacatatagc cactgatttt
    10381 tccctcgcgc accatcggta ccgcattggc ccgtacccaa taatggtcgc catttttgcg
    10441 gcgatttttc acgatgccgc tccagggctc cccttttttc agggtgaacc acatatccgc
    10501 aaacgccgct tttggcatat ccgggtgacg caccatgttg tgcggctgcc cttgtaaccc
    10561 ttgcaaggta aagccgctca cctgcacaaa agtgtcatta gcatgagtga tatagctttg
    10621 cagatcggta gtggacatca gagtggtatc gtccgccagc ggggtatttt gctgggtgac
    10681 atacggatga gaagacatgt tcgcgtccta tgcaggttat atggttgtta acttcttgtc
    10741 agagtttatg tcggccccgc tgcggttatc tttaaccgat taatttgatt tagatcgcaa
    10801 tttgcgatta aagcacaaat ctaattcctt gatttaaaat actttcactc tgttactata
    10861 cgaaaacgtt aattatcttg cccaaaatca ggcaattatc gccctgaaaa cgtgcatttg
    10921 cacagcaatc atcaaatcca tacccgacaa aaaccgtgca aaataacaac gaatgttaac
    10981 aaatagcatt aaatactgcg caaatgataa ccacatttgt gtttatcccg attttcgcga
    11041 tcgcagccgg agtggcgcaa tccctgcaat acttaaatcg gtatcatgtg atacgcgagc
    11101 ctccggagca tattttgaac aggttacctt cgagcgcatc ggctttagcg tgcagcgccc
    11161 acgccctgaa tctcattgag aagcgaacgc tggatcatga ggagatgaaa gcacttaacc
    11221 gagaggtgat tgaatacttc aaagagcatg tcaatccggg gtttttagag tatcgcaaat
    11281 ctgttaccgc cggcggggat tacggagccg tagagtggca agcgggaagt ttaaatacgc
    11341 ttgtcgacac ccagggacag gagtttatcg actgcctggg aggttttgga attttcaacg
    11401 tggggcaccg taatccagtt gtggtttccg ccgtacagaa tcaacttgcg aaacaaccgc
    11461 tgcacagcca ggagctgctc gatccgttac gggcgatgtt ggcgaaaacc cttgctgcgc
    11521 taacgcccgg taaactgaaa tacagcttct tctgtaatag cggcaccgag tccgttgaag
    11581 cagcgctgaa gctggcgaaa gcttaccagt caccgcgcgg caagtttact tttattgcca
    11641 ccagcggcgc gttccacggt aaatcacttg gcgcgctgtc ggccacggcg aaatcgacct
    11701 tccgcaaacc gtttatgccg ttactgccgg gcttccgtca tgtgccgttt ggcaatatcg
    11761 aagccatgcg cacggctctt aacgagtgca aaaaaaccgg tgatgatgtg gttgcggtga
    11821 tcctcgaacc gattcagggt gaaggtggcg taattctgcc gccgccgggc tatctcaccg
    11881 ccgtacgtaa gctatgcgat gagttcggcg cactgatgat cctcgatgaa gtacaaacgg
    11941 gcatggggcg cacgggcaag atgttcgcct gcgagcatga gaacgtacag ccggatatcc
    12001 tctgccttgc caaagcgctc ggcggcggcg tgatgccgat tggcgcgacc atcgccactg
    12061 aagaggtgtt ttcagttctg ttcgacaacc cattcctgca taccaccacc tttggcggca
    12121 acccgctggc ctgtgcggcg gcgctggcga ccatcaatgt gttgctggag cagaacttac
    12181 cggctcaggc tgagcaaaaa ggcgatatgt tgctggacgg tttccgtcaa ctggcgcggg
    12241 aatatcccga tctggtacag gaagcgcgtg gtaaagggat gttgatggca attgagtttg
    12301 ttgataacga aatcggctat aactttgcca gcgagatgtt ccgccagcgc gtactggtgg
    12361 ccggaacgct caataacgcc aaaacgatcc gcattgaacc gccactgaca ctgaccattg
    12421 aacagtgtga actggtgatc aaagcggcgc gtaaggcgct ggcggccatg cgagtaagtg
    12481 tcgaagaagc gtaatatcaa atcggatggc gatacgacgt cgccatccga tctttttata
    12541 cgacgcgtac gcccgcaggc atcatccgct ccggcgttaa caacacgctt tcactgccgt
    12601 catcggtttc cgcacacaac agcatgcatt ccgacgtttc accacgcatt ttcgcttttt
    12661 gcagattgca taagaccacc accgttttcc ccatcagctc ttcttcgctg tagtacggca
    12721 ccaggctggt cacggtttgc agtgtttttt cccccacatc aacctgtacg atgtacagct
    12781 tgtcggcgtt ttcatggcgt ttcacttcca caatctttcc gacgcgcatt tccagacgtg
    12841 caaaatcagc gtaagccacg gtttccattg tttccttcct ccgggtaaaa ttttactaaa
    12901 ctatagcaaa agtttttctc aatcctgtag gctaaaaatg gagaatgcag gcgtgatcac
    12961 attcgtgagc cgctgtgtta ccgttacagc gtcaaagaaa cgcgctttat ttactgaaaa
    13021 caggtgaccc gataagtact tcctctacaa tgggggcgca catcagggaa agtaaaaaag
    13081 gtaaacatgg caacactaaa agacatcgca atcgaagctg gcgtatccct ggcgacagta
    13141 tccagggtct taaatgacga tccgacattg aatgtgaaag aagagacgaa acatcgcatt
    13201 ctcgagatcg ccgaaaagct ggagtacaag accagtagtg cccgtaaact ccagacaggt
    13261 gcagtcaacc aacaccatat tctggctatc tacagctacc aacaggagct ggagatcaac
    13321 gatccttact atctggcgat ccgccacggc attgaaactc agtgcgaaaa gctgggcatt
    13381 gagctcacca actgttatga acacagcgga ttaccagaca ttaaaaacgt caccggtatt
    13441 ttaattgtcg gcaaacccac gcccgccctg cgcgccgccg cctgtgcgtt gaccgacaat
    13501 atctgtttta tcgactttca cgaacccggc agcggttacg atgcggtgga tatcgatctg
    13561 gcacgcatca gtaaagaaat catcgacttc tatatcaatc agggcgttaa tcgtattggt
    13621 tttattggcg gtgaagatga gccaggcaaa gcggatattc gtgaggtcgc ctttgcggaa
    13681 tatggccgac tgaaacaagt ggtacgcgaa gaggatatct ggcgcggagg cttttccagt
    13741 tcgtcgggtt atgaactggc aaaacagatg ctggcgcagg aagactatcc gaaggcactg
    13801 tttgttgctt ccgattccat tgctatcggc gtactgcggg caattcatga acgaggcctg
    13861 aacatcccac aggatatttc gcttatcagc gttaacgata tccccaccgc gcgatttacc
    13921 tttccgccgc tctccaccgt gcgcatccat tccgagatga tgggaagtca gggcgttaac
    13981 ctagtgtatg aaaaagcccg cgatggtcgc gcgctgccgc tgttagtctt cgttcccagc
    14041 aaattaaaac tgcgcggcac gacccgttaa atccccttac acactgtccg gcaatcgttt
    14101 ttgccggaca gtgctgccgt ttattttcgt gatccagtta aagtaaatgc atttatctgc
    14161 tactttttag tgaaaatttt actaagctcc ccagcaatta cacaaactac catcaccatg
    14221 aatggttccg atttctctct accgggaggc cctatgaatc gctgggaaaa cattcagctc
    14281 acccacgaaa accgacttgc gccgcgtgcg tacttttttt catatgattc tgttgcgcaa
    14341 gcgcgtacct ttgcccgcga aaccagcagc ctgtttctgc ccttaagcgg tcagtggaat
    14401 ttccactttt ttgaccatcc gctgcaagta ccagaagcct tcacctctga gttaatggct
    14461 gactgggggc atattaccgt ccccgccatg tggcaaatgg aaggtcacgg caaactgcaa
    14521 tataccgacg aaggttttcc gttccccatc gatgtgccgt ttgtccccag cgataaccca
    14581 accggtgcct atcaacgtat tttcaccctc agcgacggct ggcagggtaa acagacgctg
    14641 attaaatttg acggcgtcga aacctatttt gaagtctatg ttaacggtca gtatgtgggt
    14701 ttcagcaagg gcagtcgcct gaccgcagag tttgacatca gcgcgatggt taaaaccggc
    14761 gacaacctgt tgtgtgtgcg cgtgatgcag tgggcggact ctacctacgt ggaagaccag
    14821 gatatgtggt ggtcagcggg gatcttccgc gatgtttatc tggtcggaaa acacctaacg
    14881 catattaacg atttcactgt gcgtaccgac tttgacgaag cctattgcga tgccacgctt
    14941 tcctgcgaag tggtgctgga aaatctcgcc gcctcccctg tcgtcacgac gctggaatat
    15001 accctgtttg atggcgaacg cgtggtgcac agcagcgcca ttgatcattt ggctattgaa
    15061 aaactgacca gcgccagctt tgcttttact gtcgaacagc cgcagcaatg gtcagcagaa
    15121 tccccttatc tttaccatct ggtcatgacg ctgaaagacg ccaacggcaa cgttctggaa
    15181 gtggtgccac aacgcgttgg cttccgtgat atcaaagtgc gcgacggtct gttctggatc
    15241 aataaccgtt atgtgatgct gcacggcgtc aaccgtcacg acaacgatca tcgcaaaggc
    15301 cgcgccgttg gaatggatcg cgtcgagaaa gatctccagt tgatgaagca gcacaatatc
    15361 aactccgtgc gtaccgctca ctacccgaac gatccgcgtt tttacgaact gtgtgatatc
    15421 tacggcctgt ttgtgatggc ggaaaccgac gtcgaatcgc acggctttgc taatgtcggc
    15481 gatatcagcc gtattaccga cgatccgcag tgggaaaaag tctacgtcga gcgcattgtt
    15541 cgccatatcc acgcgcagaa aaaccatccg tcgatcatca tctggtcgct gggcaatgaa
    15601 tccggctatg gctgtaacat ccgcgcgatg taccatgcgg cgaaagcgct ggatgacacg
    15661 cgactggtgc attacgaaga agatcgcgat gctgaagtgg tcgatattat ttccaccatg
    15721 tacacccgcg tgccgctgat gaatgagttt ggtgaatacc agcatccgaa gccgcgcatc
    15781 atctgtgaat atgctcatgc gatggggaac ggaccgggcg ggctgacgga gtaccagaac
    15841 gtcttctata agcacgattg cattcagggt cattatgtct gggagtggtg cgaccacggg
    15901 atccaggcac aggacgacca cggcaatgtc tggtataaat tcggcggcga ctacggcgac
    15961 tatcccaaca actataactt ctgtcttgat ggtttgatct attccgatca gacgccggga
    16021 ccgggcctga aagagtacaa acaggttatc gcgccggtaa aaatccacgc gcgggatctg
    16081 actcgcggcg agttgaaagt cgaaaataaa ctgtggttta ccacgcttga tgactacacc
    16141 ctgcacgcag aggtgcgcgc cgaaggtgaa acgctcgcga cgcagcagat taaactgcgc
    16201 gacgttgcgc cgaacagcga agcccccttg cagatcacgc tgccgcagct ggacgcccgc
    16261 gaagcgttcc tcaacattac ggtgaccaaa gattcccgca cccgctacag cgaagccggg
    16321 cattctatcg ccacttatca gttcccgctg aaggaaaaca ccgcgcagcc agtgcctttc
    16381 gcaccaaata atgcgcgtcc gctgacgctg gaagacgatc gtttgagctg caccgttcgc
    16441 ggctacaact tcgcgatcac cttctcaaaa atgagtggca aaccgacatc ctggcaggta
    16501 aatggcgagt cgctgctgac ccgcgagcca aagatcaact tcttcaagcc gatgatcgac
    16561 aaccacaagc aggagtacga agggttgtgg cagccgaatc atttgcagat catgcaggaa
    16621 catctgcgcg actttgccgt agaacagagc gatggtgaag tgttgatcat cagccgcaca
    16681 gttattgcac cgccggtgtt tgacttcggg atgcgctgta cctacatctg gcgcatcgct
    16741 gccgatggcc aggttaacgt ggcgctttcc ggcgagcgtt acggcgacta tccgcacatc
    16801 attccgtgca tcggtttcac catggggatt aacggcgaat acgatcaggt ggcgtattac
    16861 ggtcgtggac cgggcgaaaa ctacgccgac agccagcagg ctaacatcat cgatatctgg
    16921 cgcagcaccg tcgatgccat gttcgagaac tatcccttcc cgcagaacaa cggcaaccgt
    16981 cagcatgtcc gctggacggc actgactaac cgccacggca acggtctgct ggtggttccg
    17041 cagcgcccaa ttaacttcag cgcctggcac tatacccaag aaaacatcca cgctgcccag
    17101 cactgtaacg agctgcagcg cagtgatgac atcaccctga acctcgacca ccagctactt
    17161 ggcctcggct ccaactcctg gggcagcgag gtgctggact cctggcgcgt ctggttccgt
    17221 gacttcagct acggctttac gttgctgccg gtttctggcg gagaagctac cgcgcaaagt
    17281 ctggcgtcgt atgagttcgg cgcagggttc ttttccacga atttgcacag cgagaataag
    17341 caatgaggat catcgataac ttagaacagt tccgccagat ttacgcctct agcaagaagt
    17401 ggcaacgctg tgttgaagcg attgaaaata tcgacaacat tcagcctggc gtcgcccact
    17461 ccatcggtga ctcattgact taccgcgtgg agacagactc cgcgaccgat gcgctattta
    17521 ccgggcatcg acgctatttt gaagtg
//
LOCUS       DAJTNA010000079        17465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTNA010000079 DAJTNA010000000
VERSION     DAJTNA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17465
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.080"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(36..2633)
                     /gene="topA"
                     /locus_tag="OO161_002214"
     CDS             complement(36..2633)
                     /gene="topA"
                     /locus_tag="OO161_002214"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5463269.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3013..3264
                     /locus_tag="OO161_002215"
     CDS             3013..3264
                     /locus_tag="OO161_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5463270.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3300..4349)
                     /gene="sohB"
                     /locus_tag="OO161_002216"
     CDS             complement(3300..4349)
                     /gene="sohB"
                     /locus_tag="OO161_002216"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5463271.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4569..5327
                     /locus_tag="OO161_002217"
     CDS             4569..5327
                     /locus_tag="OO161_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5463272.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEEPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5324..5915
                     /gene="cobO"
                     /locus_tag="OO161_002218"
                     /pseudo
     CDS             5324..5915
                     /gene="cobO"
                     /locus_tag="OO161_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5955..6827)
                     /gene="rluB"
                     /locus_tag="OO161_002219"
     CDS             complement(5955..6827)
                     /gene="rluB"
                     /locus_tag="OO161_002219"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5463273.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(7038..8933)
                     /locus_tag="OO161_002220"
     CDS             complement(7038..8933)
                     /locus_tag="OO161_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCR5463274.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKYPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINIGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8961..9581)
                     /locus_tag="OO161_002221"
     CDS             complement(8961..9581)
                     /locus_tag="OO161_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5463275.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9578..10459)
                     /gene="yciV"
                     /locus_tag="OO161_002222"
     CDS             complement(9578..10459)
                     /gene="yciV"
                     /locus_tag="OO161_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5463276.1"
                     /translation="MSDTNYAVIYDLHSHTIASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10597..10641
                     /gene="trpL"
                     /locus_tag="OO161_002223"
     CDS             10597..10641
                     /gene="trpL"
                     /locus_tag="OO161_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5463277.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10618..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10733..12295
                     /gene="trpE"
                     /locus_tag="OO161_002224"
     CDS             10733..12295
                     /gene="trpE"
                     /locus_tag="OO161_002224"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5463278.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSMPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVANLTKVDR
                     YSYVMYQVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12295..13890
                     /gene="trpD"
                     /locus_tag="OO161_002225"
     CDS             12295..13890
                     /gene="trpD"
                     /locus_tag="OO161_002225"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5463279.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13891..15252
                     /gene="trpCF"
                     /locus_tag="OO161_002226"
     CDS             13891..15252
                     /gene="trpCF"
                     /locus_tag="OO161_002226"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5463280.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     GLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQCFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15264..16457
                     /gene="trpB"
                     /locus_tag="OO161_002227"
     CDS             15264..16457
                     /gene="trpB"
                     /locus_tag="OO161_002227"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5463281.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMTQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     SRGEI"
     gene            16457..17263
                     /gene="trpA"
                     /locus_tag="OO161_002228"
     CDS             16457..17263
                     /gene="trpA"
                     /locus_tag="OO161_002228"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5463282.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQSEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT         4216 a         4664 c         4343 g         4242 t
ORIGIN      
        1 accctttgtt tataaaaacc tgacagaatt aaaggttatt tttttccttc aacccatttg
       61 ccatcaacat aaaatgctga ccagccagtc gcctttccgt ctttttccga agagacatat
      121 tgctgtttgg ttttacggct aaagcgaacc atggtcttat taccttccgg atcctgctgt
      181 ggcgcatcgg ccagataacg cagtttttcc ggcagacggt cgcggaagcg ataaagctct
      241 tccaccagtg gcgcacgcgt ttcacgcgat ttcgggaaag tgttggcagc caggaaaaca
      301 cccgcagcgc cgtcacgcag cacgaaataa gcatctgatt tttcgcacgg cagctcaggt
      361 aatggcaccg gatcttcttt cggtggtgcc acttcgccgt tacgtaaaat cttacgtgtg
      421 tttttacact cttcgttggt gcaggccatg tatttaccga aacgccccat tttcaggtgc
      481 atttcagagc cacatttttc acactcaacg atcgggccgt cataaccttt aatgcggaat
      541 tcgccctctt cgatctcgta accgtcgcag gttgggttat taccacagac atgcaactta
      601 cgtttcggat cgatgagata gctgtccatc gccgtgccgc atttcgggca acgacgtttt
      661 gcgcgcagcg cgttggtttc agcgtcttcg ccttccagta cgttcagcac ttcgttttcc
      721 ggcaccaggt taatggtggt tttgcaacgc tctttcggcg gcagcgcata accagaacag
      781 ccaaggaata ccccggtgct cgctgtgcga atccccattt tgcgaccaca agtcgggcag
      841 tcaatgctgg tcagaaccat ctggttcggg cgcataccac cctcttccgg atctttttca
      901 gctttatcta actgctgggt gaaatccgag aagaagtgat ccagtacagc tttccactct
      961 gcttcgtgat ttgccacctg gtcgaggctg ttttccatct gtgcggtgaa gtcgtagttc
     1021 attaactcgc ggaaattttc ttcaaggcga tcggtgacga tttcgcccat tttttccgca
     1081 tagaaacgac gattttctac tcgcacgtag ccacgatcct gaatggtcga aatgatcgac
     1141 gcataggtag atggacgacc gataccgcgt ttttccagct ctttaaccag cgatgcttca
     1201 ctgaaacggg ctggcggctt ggtaaagtgc tgggctggtg taagttcaac gagcgtcaga
     1261 gcatcgcctt tattaactgc tggtaagatg cgatcttcat cgcctttacg caacgcaggc
     1321 atcacttttg tccagccatc aaaacgcaaa atacgaccgc gtgctttcag gcggaaatcg
     1381 cccgcaccaa cggtcagcgt cgtggagtca tatttcgctg gggtcatctg gcaggctacg
     1441 aactgacgcc agattaactg gtacagtttc tgcgcatctg cttccatatc cttcagcgat
     1501 tccgccatca cattgacgtc agaaggacga atcgcttcgt gcgcttcctg tgagttttct
     1561 ttgctggcgt actgattcgg actttccggc agatatttct taccaaaatt atcgctgata
     1621 taaccgcgaa ccatatttac cgcgtcctga ctcaggttag tggagtcggt acgcatgtaa
     1681 gtgatatagc ctgcttcata caaacgctgc gccatcatca tggttttttt cacgccaaat
     1741 ccaagacggg tgctggcagc ttgttgcagc gtagaggtaa taaaaggagc gccaggttta
     1801 ctggttgtcg gtttgtcttc acgttccagc acgctgtagc gcgctttttc cagcagactt
     1861 accgcagcct gagtttgttc tttgttgacc ggacggaacg gtttgtcgtt ctgatgagtc
     1921 acctgtaacg ccaacgcttc accagatggc gtggtcgtgc tggcatcgac ttcccagaac
     1981 tcttccggca cgaacgcttt aatttcacgc tcacgctcga ccaccaggcg aaccgccacc
     2041 gactgcacac gaccggcaga cagaccacga gcgatctttt tccatagcag cggcgaaacc
     2101 atatacccca ccacgcggtc cataaagcga cgcgcctgct gggcattaac acgatcaata
     2161 ttcagctcac ccggtttgtt aaatgcctgg cggatcgcgt ttttagtaat ttcgttaaac
     2221 accactcggc tatagcgcgc atcatcaccc ccaatcactt cccgcaggtg ccatgcaatg
     2281 gcttcccctt cgcggtcaag gtcggttgcg agatagatgt ggtcggcttt ttcagccagt
     2341 tgtttcagtt cagagacgac cttctcttta ccaggcaaca cttcatagtg cgcctcccaa
     2401 ttgtgccacg ggtcaacccc catacggttg acgagagcgc cacgttcatc ctttttaggc
     2461 tttttagccg tcttggtgga ggtagagtcg gcactctttt tggcagctga gccactggtc
     2521 ggcaaatcgc ggatgtgacc gacgctggat ttcaccacgt agtcactacc cagatacttg
     2581 ttgatcgttt tggcttttgc cggggactca acgatgacaa gagctttacc catattcacc
     2641 ttacctaatt tgattcttcc aggaatgcgt cgcacgttga taaaccttcc actggcaacg
     2701 agttaccgat atggactcgt tctctgcgga tatcaacccc ttgtcgtgac tctgttcacg
     2761 aaaattcagg tgactgagtt aacaggtctt ggcctacagg cgctatagca cgacggaatt
     2821 ttggtcgaat gtcaagcaaa tctattgcca gattgacgaa agcgtcacac tgtacctgat
     2881 aaaatccgat acgcaacttt attagcatgc aaaatccaaa gttgccagta aggcgctctt
     2941 gccaccagcg tggtatattt gtctgcaagc catgcctggc gtagactaat gtccttgttt
     3001 aaggagtaaa taatgaacaa agagacacaa cccatcgacc gcgaaacgct gttgaaagag
     3061 gccaacaaaa tcattcgtga gcatgaggat acgctcgcag ggattgaagc caccggcgtc
     3121 acgcagcgta atggcgtgct ggtatttacc ggcgactact ttttagatga acaaggattg
     3181 ccaacagcca aaagcaccgc ggtgtttaat atgttcaaac acctggccca tgtgctttca
     3241 gaaaaatatc atctggttga ttaaagcaaa acgcgaggtc ttagcctcgc gtttgtcttt
     3301 tacatcaatg gcttttgacc ccgctgccac cagcgtagca acaatcgatc ggcgctctct
     3361 gccgcgctgc cggtgaatcg gtcaatgagt cgtttacgct gcatatagcg tacattaacc
     3421 acttcacggc cttccatcag gctaagaata acttcatcac tggtgttgat ttcatcaacc
     3481 aggcctttct ctaccgcctg ttgtccgtac cagtgttcac ctgttgccac ctgttcaata
     3541 tccagagacg gacgcatacg cttcacaaaa tctttaaaca actgatgcgt ttcgttcaac
     3601 tcttcgcgga atttctcccg cccttcttcg gtattttcac ccagcaacgt cagagtacgc
     3661 ttatactgcc cggcagtgtg cagttcgata tcaatatatt tgcttttcag gaagcggtta
     3721 aagttgggca tttgcgccac caccccaatg gaacccacaa tagcaaacgg tgcggaaaca
     3781 attttgtccg ccacacaggc catcatgtaa ccgccgctgg cagcgacttt gtctaccgta
     3841 acagttaaag gaatgttttt atcacgcaga cgctgcagct gcgaagccgc caacccgtaa
     3901 ccatgcacca tgccaccagg gctttccaga cggagcacaa cctgatcctg cggtttgaat
     3961 gctgcgagta ccgccgttat ctcttcacgt agcgagttca cttcatgggc gtccatgctg
     4021 cctttaaaat ccagcaccca gacgcgaggt ttactgtcag ttgccacctc gcccagtttg
     4081 gctttcgctt ttgctgcttt cgcttcttgc ttgtgctttt tcttctgcgc tttgtgccac
     4141 tgtttttgct gatgtgagcc catcagcgcc gcggccagtt cttctttcat ctccttatac
     4201 tgttcgctga gattgttgac ccgtaactcg ccacgctggc gtttattacg ttgagcaaca
     4261 ttgacaataa tggcggcaat cgccgcaatc gctagcacaa cggtaacgat tttcgccaaa
     4321 aacaaaccat attcagacaa caattccacg agtccccacc ttggttaaac aacgctttgg
     4381 ctcacagttt acaacaggca tgttaagccg tctcgcagag atgttattgc agcgaaagta
     4441 tgacaaagta tccattttgc ggtgttatcg ccccaggctg attgaaatgc ccttctgttt
     4501 caggcataaa gccccaaagt cataaagtac actggcagcg cgacagacaa cgtcgttgag
     4561 gagtagccat gcattaccag ccaaaacaag atttactcaa tgatcgcatt atcctggtga
     4621 cgggagccag cgatgggatt ggtcgtgaag ccgcgatgac gtatgcacgc tatggtgcga
     4681 cagtgattct gttgggccgt aatgaagaaa aattacgtca ggtagccagc cacataaacg
     4741 aagaaactgg gcgtcagcca cagtggttta ttctcgattt gctgacctgc acgtccgaaa
     4801 attgccaaca actggcacag cgcattgtcg ttaattatcc gcgtctggat ggtgttttgc
     4861 ataatgccgg attgctcggc gatatttgcc caatgagcga acaaaatccg caggtctggc
     4921 aggacgtcat gcaggtcaac gttaatgcca cctttatgct cacccaggca ctgcttcctt
     4981 tattactcaa atcggacgcc ggttcactgg tctttacttc atcaagcgtt ggacgtcagg
     5041 gacgagctaa ctggggtgca tatgcagcgt cgaaatttgc caccgaaggg atgatgcagg
     5101 tactggccga tgaatatcag cagcgcctgc gcgtcaactg cattaaccca ggcggtactc
     5161 gcaccgcaat gcgcgcaagc gccttcccga ccgaagagcc acagaaactt aaaacacccg
     5221 ctgatatcat gccgctctac ctctggctga tgggcgatga cagccgccgt aaaaccggca
     5281 tgacctttga cgcccaaccg ggccgtaaac caggaatttc ccaatgagtg atgaacgcta
     5341 ccaacagcgt cagcagcgag tgaaagaaaa agtagatgct cgtgtggccc aggccccagg
     5401 atgaacgcgg tattatcatc gtctttaccg gcaatggaaa aggcaaaacc accgcggcat
     5461 ttggtacggc aacacgcgca gttggtcacg gaaaaaaagt gggcgtcgtg cagtttatta
     5521 aaggcacctg gcctaatggc gaacgcaatc tgctggaacc acatggcgtt gagtttcagg
     5581 tgatggcaac gggctttacc tgggatacac aaaaccgcga gtctgatacc gccgcctgcc
     5641 gcgaagtctg gcaacatgca aagcggatgc ttgctgattc ctcactggat atggttttgc
     5701 ttgatgaact gacgtatatg gtggcgtatg actatttgcc actggaagaa gtggtgcagg
     5761 cgttaaatga acgtccacat caacagacgg tgattatcac gggtcgaggc tgtcatcggg
     5821 atattcttga actggcggat acggtaagtg agctgcgccc catcaaacat gcgtttgatg
     5881 ccggtgttaa ggcgcaaata gggattgatt attaatctgt aaaaatgccg gatatgacgt
     5941 ccggcatact ttgattaacc gttattacgc ccgccagaac gacgaccgcc gctcacctga
     6001 ctgtgacgtt tcaccgcacg acgaatctga ttagccttca tgcgacgacg gtctttttct
     6061 acagcgactt tagagctggt ttctggcgga agctccacca gttcgcgcag atagttagtc
     6121 tgggcgaggt ccagctccgt ccagccaccg cgtggcagac cttttggcag tgggatatca
     6181 ccgtaacgaa cacggatcag gcggctcacc tgcacaccaa ccgcttccca cagacgacga
     6241 acttcacggt tacggccttc ggtcagagtc acgttgtacc actggttgat cccttcgccg
     6301 ccgctgaact tgatggtttt aaaagctgcc ggaccatctt ccagttgcac gccacgactc
     6361 aaatcacgca gtttcgcgtc gtcaacctga ccaaatacac gcacggcgta ttcacgttca
     6421 acttcacggc ttggatgcat taaacggttt gccagttcac catcggtggt gaacagcagc
     6481 agaccacagg tgttaacgtc cagacgacct acggcaatcc agcgtgcgcc acgcagcttt
     6541 ggcagacggt caaacaccgt cggacgccct tccggatcgt tacgggtaca caactcacct
     6601 tccggtttgt aataggccag aacgcgacaa atttgttcag cggactcacg taccgaaatc
     6661 agatgaccat cgatacgaat tttcagccca ggggtaactt caacgcgatc gccgagttta
     6721 gcaattttgc catcaacact cacacgaccg gcttcaataa tagattcgat ttcacggcgg
     6781 gagccgtggc cagcgcgggc cagcactttc tgtaattttt cgctcataga gcttcctttt
     6841 ggtgtcgcct tcccaggcgt ctgatatgct tttgtgcaac agtgagtcag aacttacatc
     6901 tgactcactg atagtaaagt ctgtttgaac tcttcaggcc ttaagtaaac gactcgacgt
     6961 ttatcggcct ggctttgagg cgcaacatac tacactatct ttatggttaa agcttgtttc
     7021 gattaacatc agcttaatta ccagccacca ccgcctccgc cacccgcgcc gccgccagca
     7081 ccaccaccgg aataacttcg ccccaacaaa ctggtattgg ccccgcaact taggtgcgtc
     7141 tgtaatgaga ataaaacgcc gttacgggca atctctggag cgcgaatcgc cgaagaaagc
     7201 gaaggggcaa tgcgtaacgc ccattccctc cccaaatttg ccgatatagc ccaacctagc
     7261 aacgcgtaat ccagtttccg tgtttcccct ttccgccgac gtccactgtg tgttgctgcc
     7321 tctttacgcg caagatagtt aacgatagct tcaccgcggg cataacgttg ctgcccagtt
     7381 tgggtaaact ttggcagaaa aatatacccc atgccagtga gataaccggt caggcaaaca
     7441 gcgctaaaat aacccgctgg catataccag taaaagaata catctccgat attgatatag
     7501 agagaataga aggccacgcc tgaacaaatc agtggtaaaa acaaagtgat gattgcacga
     7561 ttaacgcaag gcttgtcatc atcgcgggca aacaaaagct caagggaaca aaataaaaaa
     7621 ggaataatcc agcagatcat aataaacata tcgcctacca gactcattcc tgcccaacct
     7681 acgggactca ggttaccgta gagaatgatc gccaatattg ccagtccacc ccattgcaaa
     7741 aacgtatcag gccggtaaaa caaagacttt ttacgcggca gataaaattt ctgcatccgt
     7801 aaaaatgccc tgcgtaagac tctgttgcgt ctccccgtaa gaacgacctt atcgccttta
     7861 cgaaataacg cttccagtaa taactgctct gcgggcagta atacctcatt atttctggtc
     7921 gcgccttcat taacacaaat caggatttgc tggttcttat catactgatc ttccagatga
     7981 atttttccct tcacgatcag atttacgata tcggcacaga aacctttatc gtcgtaaacc
     8041 agcttcgcat cgagacgtaa catgccgggc gtgtaatctg gcggaatgac atcggtttca
     8101 atcacatcta ccggcgtaaa ttgcggcctg cgcttccaga gatataacca tccacaaacc
     8161 aggaatagac acggaaacca aattaacaac gatgacgtgg agggtaaaag aagatggctg
     8221 aaaatattcg tcgtttgtgg agccggggca ttgctaagta acgcgtgagg ccagcggtag
     8281 agtaccgtga aatcttcacg ataaaacgga tcgcgggatt caattcttcc gtcctcaagg
     8341 atgcggccat ttcgatatgt gtcgccctct tctccagtaa agagatcgat ttcgctaaat
     8401 ggattacccg cgatatcagg gtacttgagt gaaaatcggg tcttatagat ttcaaacggc
     8461 cagtggttac cagtcacgtt ccagattaac agatctgaat ctccctcacg caggaaagca
     8521 tttttaacct gataatgaat ttgataatga tagtcgcctt gaggcaagaa acgttgttta
     8581 tcgcccacga caatactcat taacccgata tgccagtcaa gatgccaggg ttcaggaata
     8641 ccatcacgag tgacgccaac aatatgatag ttcagcagtg cagcgtcccc atcaggctgc
     8701 atccagagtc gtggtaaatc acgataaaat ccgtgcttaa tctgctgatt tcgcgcctga
     8761 aaatgaatat tttctttgat gtcgagcacc ccatccggtt gcagagtggc ttcaacatca
     8821 aagagcgaga tcccttcttc ataagaaggg atctccgggt tttctgcata acttagagat
     8881 gatacaaaca gccctgctat caacagcaaa atgcagcgaa acttccctgc catagtcttc
     8941 catccgtgga acgcaatttg ttataagaaa ggcttcacat cacccacgcc ttcacgcacc
     9001 acgaccggcg tatcatcggt aagatcaatg accgttgtcg gtttctgtcc gagatagccg
     9061 ccgtggataa tcaaatccac ctgtttttcc agacggtctt taatttcttc tggatcggat
     9121 tcggtaaatt cgctgcctgg cagcattagc gaagtggaaa gcatcggttc gcccagtgct
     9181 tcaagcaacg cctgcgcaat cgggttagaa ggcacacgca taccgatggt tttgcgtttt
     9241 tcctgcaaca gacggcgtgg cacctccttc gtccccttca ggatgaaggt atagttgccc
     9301 ggcgtgttgt ttttcattaa acgaaacgca acgttatcca cgaatgaata ggtcgaaagt
     9361 tcagaaagat cgcgacacat cagggtaaag ttgtgaccgt ccggcagctg gcgaatacga
     9421 caaatacgct ccatcgcgtt tttatcttca attttacagc cgagcgcata gccggaatca
     9481 gttggataaa caatcacccc gcctttacgc acgatctcca ccgcctggtt gatcagacgt
     9541 tgctgcgggt tatcaggatg aatataaaaa aactggctca taattccctc tctgtggtgt
     9601 tctgcggctg ttcccatagc tgccagacgc cttcaacgcc tgcgggcagc cagagtttac
     9661 gacccagttc gatccacggg catggctgat gaaaatcaga tccttgtgat gcccataaat
     9721 gatgctgacg cgcaagtgtc gtcaactggg tgcgttcatt gggcgattgc tggcactgcg
     9781 cgacttccat cgcgtcgccg tggtgttcgg caaaatgcgc aaccaatctt ttcagccatt
     9841 tagcactaag attgtaccgc ccaggatgag ctagcaccgc cttaccgcca gaatgatgaa
     9901 tgacatcaat agcttgttct attgtacacc actgtggcgg aacgtatccg gttttcccgc
     9961 gcgccagata ctttttaaag acatctgcca ttgaactggc tttgccgcac tcaacaagga
    10021 aacgcgcaaa atgaccgcgc gtcactgcgc cgccctgcgc cagtcgttgt gcgccttcca
    10081 gcgccccagg tatttgcgct ttttcaagtc tttcggcaat ttgctgcgcc cgctgattgc
    10141 gccgttctgt ctgctgcgcg aggaactcac acattagcgg atgagtaata tcaatattca
    10201 gcccgacaat atgaatttca tgattttccc agaccgtgga aatttccacg ccgggaataa
    10261 gattcagcgc cagtcccgaa cgtgaaattt cctctcttgc tggcgcgatt gcagctgtgg
    10321 tgtcatggtc ggtgatcgcc agggtgccga cgcgcatctc gactgcacgg tgcaccaatg
    10381 cttctggcgt caggcagcca tcggaagcta tggtatggct gtgcaggtcg taaatcactg
    10441 cgtaattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc
    10501 ataacggttc tggcaaatat tctgaaatca gctgttgaca attaatcatc gaactagtta
    10561 actagtacgc tagttcacgt aaaaagggta tcgacaatga aagcaatttt cgtactgaaa
    10621 gcttggtggc gcacttcctg aaacgggcag tgtattcacc atgcgtaaag caatcagata
    10681 cccagcccgc ctaatgagcg ggcttttttt tgaacaaaat tagagaataa caatgcaaac
    10741 acaaaaaccg actctcgaac tgctaacctg cgaaggcgct tatcgcgaca acccgactgc
    10801 gctttttcac cagttgtgtg gggatcgtcc ggcaacgctg ctgctggaat ccgcagatat
    10861 cgacagcaaa gatgatttaa aaagcctgct actggtagac agtgcgctgc gcattacagc
    10921 attaggtgac actgtcacaa ttcaggcact ttccggcaac ggcgaagccc tgctgacact
    10981 actggataac gccctgcctg cgggtgtgga aaatgaacaa tcaccaaact gccgtgtgct
    11041 gcgcttcccc cctgtcagtc cactgctgga tgaagacgct cgcttatgct ccctttcggt
    11101 ttttgacgct ttccgtttat tgcagaatct gttgaatgta ccgaaggaag aacgagaagc
    11161 aatgttcttc ggcggcctgt tctcttatga ccttgtggcg ggatttgaag atttaccgca
    11221 actgtcagcg gaaaataact gccctgattt ctgtttttat ctcgctgaaa cgctgatggt
    11281 gattgaccat cagaaaaaaa acacccgcat tcaggccagc ctgtttgctc cgaatgaaga
    11341 agaaaaacaa cgtctcactg ctcgcctgaa cgatcttcgc cagcagctga ccgaagccgc
    11401 gccgccgctg ccggtggttt ccatgccgca tatgcgttgt gaatgtaacc agagcgatga
    11461 agagttcggt ggcgtggtgc gtttgttgca aaaagcgatt cgcaccggag aaattttcca
    11521 ggtggtgccg tctcgccgtt tctctctgcc ctgcccgtca ccgctggcgg cctattacgt
    11581 gctgaaaaag agtaatccca gcccgtacat gttttttatg caggataatg atttcaccct
    11641 gtttggcgcg tcgccggaaa gttcgctcaa gtatgacgcc accagccgcc agattgagat
    11701 ctacccgatt gccggaacac gcccgcgcgg tcgtcgcgcc gatggttcac tggacagaga
    11761 cctcgacagc cgcatcgaac tggaaatgcg taccgatcat aaagagcttt ctgaacatct
    11821 gatgctggtg gatctcgccc gtaatgatct ggcacgcatt tgcacccccg gcagccgcta
    11881 cgtcgccaat cttaccaaag ttgaccgtta ctcttacgtg atgtaccagg tctcccgcgt
    11941 ggtcggtgag ctgcgccacg atctcgacgc cctgcacgct taccgcgcct gtatgaatat
    12001 ggggacgtta agcggtgcgc cgaaagtacg cgccatgcag ttaattgccg aggcggaagg
    12061 tcgtcgccgc ggcagctacg gcggcgcggt aggttatttc accgcgcatg gcgatctcga
    12121 cacctgcatt gtgatccgct cggcgctggt ggaaaacggt atcgccaccg tgcaagccgg
    12181 tgctggcata gtccttgatt ctgttccgca gtcggaagcc gacgaaaccc gtaataaagc
    12241 ccgcgctgta ctgcgcgcta ttgccaccgc gcatcatgca caggagactt tctgatggct
    12301 gacattctgc tgctcgataa tatcgactct tttacgtaca acctggcaga tcagttgcgc
    12361 agcaatgggc ataatgtggt gatttaccgc aaccatattc cggcgcagac cttaattgaa
    12421 cgcctggcga cgatgagcaa tccggtgctg atgctttctc ctggccccgg tgtgccgagc
    12481 gaagccggtt gtatgccgga actcctcacc cgcttgcgtg gcaagctgcc cattattggc
    12541 atttgcctcg gacatcaggc gattgtcgaa gcttacgggg gctatgtcgg tcaggcgggc
    12601 gaaattctcc acggtaaagc ctccagcatt gaacacgacg gtcaggcgat gtttgccgga
    12661 ttaacaaacc cgctgccagt ggcgcgttat cactcgctgg ttggcagtaa cattccggcc
    12721 ggtttaacca tcaacgccca ttttaatggc atggtgatgg cagtacgtca cgatgcggat
    12781 cgcgtttgtg gattccagtt ccatccggaa tccattctta ctacccaggg cgctcgcctg
    12841 ctggaacaaa cgctggcctg ggcgcagcgg aaactagagc caaccaacac gctgcaaccg
    12901 attctggaaa aactgtatca ggcgcagacg cttagccagc aagaaagcca ccagctgttt
    12961 tcagcagtgg tacgtggcga actgaaaccg gaacaactgg cggcggcgct ggtgagcatg
    13021 aaaattcgcg gcgagcaccc gaacgagatc gccggagcag caaccgcgtt actggaaaac
    13081 gccgcgccgt tcccgcgccc ggattatctg tttgccgata tcgtcggcac cggtggtgac
    13141 ggcagtaaca gcatcaatat ttccaccgcc agtgcgtttg tcgccgcggc ctgtgggctg
    13201 aaagtggcga aacacggcaa ccgtagcgtc tccagtaaat ctggttcgtc cgatctgctg
    13261 gcggcgttcg gtattaatct tgatatgaat gccgataaat cgcgccaagc gctggatgag
    13321 ttaggcgtat gtttcctctt tgcgccgaaa tatcacaccg gatttcgcca tgcgatgccg
    13381 gttcgccagc aactgaaaac ccgcactctg ttcaacgtgc tgggaccatt gattaacccg
    13441 gcgcatccgc cgctggcgtt aattggtgtt tatagtccgg aactggtgct gccgattgcc
    13501 gaaaccttgc gcgtgctggg gtatcaacgc gcggcggtag tgcacagcgg cggtatggat
    13561 gaagtttcat tacacgcgcc gacaatcgtt gccgaactgc atgacggtga aattaagagc
    13621 tatcagctaa ctgcagaaga ttttggcctg acaccctacc accaggagca actggcaggc
    13681 ggaacaccgg aagaaaaccg tgacatttta acacgcttgt tacaaggtaa aggcgacgcc
    13741 gctcacgaag cagccgtcgc ggcgaatgtc gccatgttaa tgcgcctgca tggccatgaa
    13801 gatctgcaag ccaatgcgca aaccgttctt gaggtactgc gcagtggttc cgcttacgac
    13861 agagttaccg cactggcggc acgagggtaa atgatgcaaa ccgttttagc gaaaatcgtc
    13921 gcagacaagg cgatttgggt agaaacccgc aaacagcagc aaccgctggc cagttttcag
    13981 aatgaggttc agccgagcac gcgacatttt tatgatgcgc tacagggtgc acgcacggcg
    14041 tttattctgg agtgcaagaa agcgtcgccg tcaaaaggcg tgatccgtga tgatttcgat
    14101 ccggcacgca ttgccgccat ttataaacat tacgcttcgg caatttcggt gctgactgat
    14161 gagaaatatt ttcaggggag ctttgatttc ctccccatcg tcagccaaat cgccccgcag
    14221 ccgattttat gtaaagactt cattatcgac ccttaccaga tctatctggc gcgctattac
    14281 caggccgatg cctgcttatt aatgctttca gtactggatg acgaccaata tcgccagctt
    14341 gcagccgtcg ctcacagtct ggagatgggg gggctgaccg aagtcagtaa tgaagaggaa
    14401 ctggagcgcg ccattgcatt aggggcaaag gtcgttggca tcaacaaccg cgatctacgt
    14461 gatttgtcga ttgatctcaa ccgtacccgc gagcttgcgc cgaaactggg gcacaacgta
    14521 acggtaatca gcgaatccgg catcaatact tacgctcagg tgcgcgagtt aagccacttc
    14581 gctaacggtt ttctgattgg ttcggcgttg atggcccatg acgatttgca cgccgccgtg
    14641 cgccgggtgt tgctgggtga gaataaagta tgtggcctga cgcgtgggca agatgctaaa
    14701 gcagcttatg acgcgggcgc gatttacggt gggttgattt ttgtcgcgac atcaccgcgt
    14761 tgcgtcaacg ttgaacaggc gcaggaagtg atggctgcgg caccgttgca gtatgttggc
    14821 gtgttccgca atcacgatat tgccgatgtg gtggacaaag ctaaggtgtt atcgctggcg
    14881 gcagtgcaac tgcatggtaa tgaagatcag ctgtatatcg atacgctgcg tgaagctctg
    14941 ccagcacatg ttgccatctg gaaagcatta agcgtcggtg aaaccctgcc cgcccgcgag
    15001 tttcagcacg ttgataaata tgttttagac aacggccagg gtggaagcgg gcaatgtttt
    15061 gactggtcac tattaaatgg tcaatcgctt ggcaacgttc tgctggcggg gggcttaggc
    15121 gcagataact gcgtggaagc ggcacaaacc ggctgcgccg gacttgattt taattctgct
    15181 gtagagtcgc aaccgggcat caaagacgca cgtcttttgg cctcggtttt ccagacgctg
    15241 cgcgcatatt aaggaaagga acaatgacaa cattacttaa cccctatttt ggtgagtttg
    15301 gcggcatgta cgtgccacaa atcctgatgc ctgctctgcg ccagctggaa gaagcttttg
    15361 tcagcgcgca aaaagatcct gaatttcagg ctcagttcaa cgacctgctg aaaaactatg
    15421 ccgggcgtcc aaccgcgctg accaaatgcc agaacattac ggctgggacg aacaccacgc
    15481 tgtatctgaa gcgcgaagat ttgctgcacg gcggcgcgca taaaactaac caggtgctcg
    15541 gtcaggcttt actggcgaag cggatgggta aaaccgaaat catcgccgaa accggtgccg
    15601 gtcagcatgg cgtggcgtcg gcccttgcca gcgccctgct cggcctgaaa tgccgtattt
    15661 atatgggtgc caaagacgtt gaacgccagt cgcctaacgt ttttcgtatg cgattaatgg
    15721 gtgcggaagt gatcccggta catagcggtt ccgcgacgct gaaagatgcc tgtaacgagg
    15781 cgctgcgcga ctggtccggc agttatgaaa ccgcgcacta tatgctgggc accgcagctg
    15841 gcccacatcc ttatccgacc attgtgtgtg agtttcagcg gatgattggc gaagaaacga
    15901 aagcgcagat tctggaaaga gaaggtcgcc tgccggatgc cgttatcgcc tgtgttggcg
    15961 gcggttcgaa tgccatcggc atgtttgcag atttcatcaa cgaaaccaac gtcggcctga
    16021 ttggtgtgga gccgggtggt cacggtatcg aaactggcga gcacggcgca ccgctaaaac
    16081 atggtcgcgt gggcatttat ttcggtatga aagcgccgat gacgcaaacc gaagacgggc
    16141 agattgaaga atcttactcc atctccgccg gactggattt cccgtctgtc ggcccacaac
    16201 acgcgtatct taacagcact ggacgcgctg attacgtgtc tattaccgat gatgaagccc
    16261 ttgaagcctt caaaacgctg tgcctgcacg aagggattat cccggcgctg gagtcttccc
    16321 acgccctggc ccatgcgctg aaaatgatgc gcgaaacccc ggaaaaagag cagctactgg
    16381 tggttaacct ttccggtcgc ggcgataaag acatcttcac cgttcacgat attttgaaat
    16441 cacgagggga aatctgatgg aacgctacga atctctgttt gcccagttga aggagcgcaa
    16501 agaaggcgca ttcgttcctt tcgtcacgct cggggatccg ggcattgagc agtcattgaa
    16561 aattatcgat acgctaattg aagccggtgc tgacgcgctg gagttaggta tccccttctc
    16621 cgacccactg gcggatggcc cgaccattca aaacgccact ctgcgcgcct ttgcggcagg
    16681 tgtgactccg gcacaatgtt ttgaaatgct ggcactgatt cgccagaaac acccgaccat
    16741 tcccattggc ctgttgatgt atgccaatct ggtgtttaac aaaggcattg atgagtttta
    16801 tgcccagagc gagaaagtcg gcgtcgattc ggtgctggtt gccgatgtgc cagttgaaga
    16861 gtccgcgccc ttccgccagg ccgcgttgcg ccataatgtc gcacctatct tcatctgccc
    16921 gccaaatgcc gatgacgacc tgctgcgcca gatagcctct tacggtcgtg gttacaccta
    16981 tttgctgtca cgagcgggcg tgaccggcgc agaaaaccgc gccgcgttac ccctcaatca
    17041 tctggttgcg aagctgaaag agtacaacgc tgcacctcca ttgcaggggt ttggtatttc
    17101 cgccccggat caggtaaaag cagcgattga tgcaggagct gcgggcgcga tttctggttc
    17161 ggccattgtt aaaatcatcg agcaacatat taatgagcca gagaaaatgc tggcggcact
    17221 gaaagttttt gtacaaccga tgaaagcggc gacgcgcagt taatcccaca gccgccagtt
    17281 ccgttggcgg cattttaact ttctttaatg aagccggaaa aatcctaaat tcatttaata
    17341 tttatctttt taccgcttcg cttacccctg tcgaacgtca acttacgtca tttttccgcc
    17401 caacagtaat ataatcaaac aaattaatcc cgcaacataa caccagtaaa atcaataatt
    17461 ttctc
//
LOCUS       DAJTNA010000080        17355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTNA010000080 DAJTNA010000000
VERSION     DAJTNA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17355
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.081"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(72..131)
                     /gene="ibsA"
                     /locus_tag="OO161_002229"
     CDS             complement(72..131)
                     /gene="ibsA"
                     /locus_tag="OO161_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR5463283.1"
                     /translation="MRKHVIILVILLVISFQAY"
     gene            complement(403..459)
                     /locus_tag="OO161_002230"
     CDS             complement(403..459)
                     /locus_tag="OO161_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5463284.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(731..787)
                     /locus_tag="OO161_002231"
     CDS             complement(731..787)
                     /locus_tag="OO161_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5463285.1"
                     /translation="MMKILIIVVLLVISFPAY"
     gene            1066..2313
                     /gene="mdtA"
                     /locus_tag="OO161_002232"
     CDS             1066..2313
                     /gene="mdtA"
                     /locus_tag="OO161_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR5463286.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELEAQQTLVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPSAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            2313..5435
                     /gene="mdtB"
                     /locus_tag="OO161_002233"
     CDS             2313..5435
                     /gene="mdtB"
                     /locus_tag="OO161_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR5463287.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVNDTQFELMMAIALVVMIIYLFLRNIPATIIPGFAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            5436..8513
                     /gene="mdtC"
                     /locus_tag="OO161_002234"
     CDS             5436..8513
                     /gene="mdtC"
                     /locus_tag="OO161_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5463288.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQATINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            8514..9929
                     /locus_tag="OO161_002235"
     CDS             8514..9929
                     /locus_tag="OO161_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5463289.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASCHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFITPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            9926..11329
                     /gene="baeS"
                     /locus_tag="OO161_002236"
     CDS             9926..11329
                     /gene="baeS"
                     /locus_tag="OO161_002236"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5463290.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLSSMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVISSPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            11326..12048
                     /gene="baeR"
                     /locus_tag="OO161_002237"
     CDS             11326..12048
                     /gene="baeR"
                     /locus_tag="OO161_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5463291.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            12239..12571
                     /locus_tag="OO161_002238"
     CDS             12239..12571
                     /locus_tag="OO161_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5463292.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            12718..14079
                     /gene="yegQ"
                     /locus_tag="OO161_002239"
     CDS             12718..14079
                     /gene="yegQ"
                     /locus_tag="OO161_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5463293.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(14410..14727)
                     /locus_tag="OO161_002240"
     CDS             complement(14410..14727)
                     /locus_tag="OO161_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463294.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            15133..16032
                     /gene="yegS"
                     /locus_tag="OO161_002241"
     CDS             15133..16032
                     /gene="yegS"
                     /locus_tag="OO161_002241"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5463295.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEVLDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVISIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(16114..16887)
                     /locus_tag="OO161_002242"
     CDS             complement(16114..16887)
                     /locus_tag="OO161_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5463296.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(16993..>17355)
                     /locus_tag="OO161_002243"
     CDS             complement(16993..>17355)
                     /locus_tag="OO161_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5463297.1"
                     /translation="LRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTS
                     ATFGKILRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQ
                     AVRDIARNAMPGKVLLIP"
BASE COUNT         3941 a         4635 c         4592 g         4187 t
ORIGIN      
        1 gacgggactt tttatacccc gcaggggtca taacatgctt gacaacttaa taggggcacc
       61 ccctggtgtt cttagtaagc ctggaagcta atcactaaga gtatcaccag tatgatgacg
      121 tgcttcctca taaccctttc cttatttaag gccccttcct cgggaggggc tttcccgttt
      181 cagcatcccg ctgaaatcgt cggcttacct cctttcgcca tgcatgcagt ctatcgctga
      241 cttgcggata aaatagtttc cagtgtgatt attgcgtaac gtctcgcaaa gaatatttgc
      301 tggcaggatc gcagactgca aagcctgcag attgacaatc tgaccgtgaa ggcatacttt
      361 caggagtgag ggttgagcgg ggtttccccc gccctggtag tcttagtaag ctggccagct
      421 aatgactaag agcaccacga tgatgagtag cttcatcatg accctttcct tatttaaggc
      481 cccttcctcg ggaggggctt tcccgtttca gcatcccgct gaaatcgtcg gcttacctcc
      541 tttcgccctg aatacagtct atcgctaacg cgtgaacaaa atagtttcct ctgtgattat
      601 tgcgtaacga ctcgcaaaga atatttgctg ccaggatcgc agactgcaaa gcctgcggat
      661 tgacaatctt atcgtgaagg catactttca ggagtgaggg tagagcgggg tttcccccgc
      721 cctggtagtc ttagtaagcc gggaagctaa tgactaagag caccacgatg atgagtatct
      781 tcatcatgac cctttcctta tttaagaccc cttcctcggg aggggctttc ccgtttcagc
      841 atcccgctga aatcgtcggc ttacctcctt tcgccaagca tgcagtctat cgctaacgcg
      901 tagataaaat agtttcctgt gttattactg gatgcgtgct cgcaaatgtg cccgccattc
      961 agacgattcc cgatagtgtt tcataattcc tccatttttc tcccttattg gctggctaca
     1021 ctagtatcat tccgcgaaac gtttcaggaa gagaaactct taacgatgaa aggcagttat
     1081 aaatcccgtt gggtaatcgt aatcgtggtg gttatcgccg ccatcgccgc attctggttc
     1141 tggcaaggcc gcaatgactc ccggagtgca gccccagggg cgacgaaaca agcgcagcaa
     1201 tcgccagcgg gtggtcgccg tggtatgcgt tccggcccat tagccccggt tcaggcggcg
     1261 accgccgtag aacaggcagt tccgcgttac ctcaccgggc ttggcaccat taccgccgct
     1321 aatactgtta cggtgcgcag ccgcgtggac ggtcaactga tggcgttaca tttccaggaa
     1381 ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca gttcaaagtt
     1441 gcattagcac aagcccaggg ccaactggca aaagataaag ccacgcttgc taacgcccgc
     1501 cgtgacctgg cgcgttatca gcaactggca aaaaccaatc tcgtatcccg tcaggagctg
     1561 gaggcccaac agacgctggt cagtgaaacc gaaggcacca ttaaggctga tgaagcaagc
     1621 gtcgccagcg cgcagctgca actcgactgg agccgcatca ccgcaccagt cgatggtcgc
     1681 gttggtctca agcaggttga tgttggtaac caaatctcca gtggtgatac caccgggatc
     1741 gtggtgatca cccagacgca tcctatcgat ttgctcttta ccctgccgga aagcgatatc
     1801 gctaccgtcg tgcaggcgca gaaagccgga aaaccgctgg tggtagaagc ctgggatcgc
     1861 accaactcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca aatcgatgcc
     1921 acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc gctgtttccc
     1981 aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt agtgatccca
     2041 tcagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa tagtgaaaac
     2101 aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt ggtgatccgt
     2161 gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct gaccgaaggg
     2221 gcgaaagtgg aagtggtgga agcccagagc gccaccactc cagaagagaa agccaccagc
     2281 cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg agcagcacag
     2341 gcggcccgtc tcgcctgttt attatgcgtc ctgtcgccac cacgctgctg atggtggcga
     2401 tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg ccggaagtgg
     2461 actatccgac cattcaggta gtcacgcttt acccaggtgc aagcccggat gtcatgacct
     2521 ctgccgttac cgcgccgctc gagcgccagt tcgggcagat gtctggcctg aaacagatgt
     2581 cgtcgcaaag ttccggcggt gcgtcagtta tcaccttaca gttccagcta acattaccgc
     2641 tcgatgtcgc cgagcaggaa gtgcaggccg cgattaacgc cgccactaac ctgctaccga
     2701 gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg ccgatcatga
     2761 cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg gtggaaaccc
     2821 gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt tccggcggtc
     2881 agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc ggcctgacca
     2941 gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa ggtagcctcg
     3001 acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc gccgaagagt
     3061 atcgccagct aatcatcgcc taccagaacg gcgcgccgat tcgtcttggc gatgtcgcaa
     3121 ccgtagagca aggtgcagaa aacagctggc ttggcgcatg ggcgaacaaa gaacaggcca
     3181 ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc gacagcatcc
     3241 ggcagatgct gccacagctc accgagagtc tgccgaaatc ggtgaaggta acagtgcttt
     3301 ccgatcgcac caccaatatc cgcgcatcgg tcaacgatac ccagtttgaa ttgatgatgg
     3361 ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg gcgaccatca
     3421 ttcccggctt cgcagtgccg ctgtcgttaa tcggcacctt cgcggtcatg gtgtttctcg
     3481 atttttcaat caataacctg acgctgatgg cgttaactat cgccaccggg ttcgtggtcg
     3541 atgacgccat cgtggtgatc gaaaatattt cccgctatat cgaaaaaggc gaaaaaccgt
     3601 tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg ctgaccttct
     3661 cgctgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg cgactgttcc
     3721 gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg tcgctgaccc
     3781 tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa cagaaccgct
     3841 tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt cgtggactgg
     3901 cgaaagtact gaatcatccg tggctgacct taagcgtggc actcagcacg ctgctgctta
     3961 gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac aacggcatta
     4021 ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc cagcgacaac
     4081 gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc tcatttgttg
     4141 gcgttgatgg cactaacccg tcgctgaaca gtgcgcgttt gcagatcaac ctcaaaccgt
     4201 tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg gcggtagata
     4261 aagtgccggg cgtcgatctc ttcctgcaac caacgcagga tctgactatt gatactcagg
     4321 tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg ctcagtacct
     4381 gggtgccaca gttgatggaa aaactccagc agctgccaca gctatctgat gtctccagcg
     4441 actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc gccagccgtc
     4501 tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt cagcggctga
     4561 tttccactat ttatactcag gccaaccagt atcgcgtggt gctggagcac aacaccgaaa
     4621 ataccccagg gcttgcggcg ctagatacca ttcgcctgac cagcagcgac ggcggcgtgg
     4681 tgccgttaag ctcaattgcc aaagttgaac agcgttttgc gccgctttcc atcaaccatc
     4741 tggatcagtt cccggtaacg accatctctt ttaacgtgcc agataactat tcgctgggcg
     4801 atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg gatatcacca
     4861 cgcagttcca gggcagcacc ctcgccttcc agtcggcgct gggcagcact gtctggctga
     4921 ttgtcgcggc ggtggtggcg atgtatatag tgctcggcat tctgtacgag agctttattc
     4981 acccgatcac cattctctcg acgctaccca cggcaggtgt tggcgccctg ctggcgttaa
     5041 tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg ctgatcggta
     5101 tcgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag cgcgagcaag
     5161 gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt ccgatcctga
     5221 tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc ggggtcggcg
     5281 cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg cctgattgtc agccaggtgc
     5341 tgacgctgtt caccacgccg gtgatttatc tgctgtttga ccgcctggca ttgtggacca
     5401 aaagtcgctt tgcccgtcat gaagaggagg cgtaagtgaa gttttttgcc ctcttcattt
     5461 accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc atactgggct
     5521 tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt atggtcagcg
     5581 cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg ccgctggagc
     5641 gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg ctcggcagca
     5701 cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg cgtgatgtgc
     5761 aggcgacgat caacgccgca caaagtttgc tgcccagtgg gatgcccagc cgtccgacct
     5821 atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca tccgatactt
     5881 attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg atttcgcaaa
     5941 tcgacggtgt tggtgatgtc gatgtcggag gcagctcact gcccgccgta cgcgtcgggc
     6001 tgaatccgca ggcgctgttt aatcagggcg tctcgctgga cgacgtacgc actgccatca
     6061 gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac cgctggcaga
     6121 tccagaccaa tgatgagcta aaaaccgccg ccgaatatca gccgctgatt attcattaca
     6181 acaacggcgg cgcggttcgt ctgggcgatg tggcgacagt gaccgactct gtacaggatg
     6241 tacgtaacgc cgggatgacc aatgccaaac cggctatttt gctgatgatc cgcaagctgc
     6301 cggaagccaa tattatccag acggttgaca gcatccgggc aaaattaccg gagttgcagg
     6361 aaaccattcc ggcggcgatt gatctgcaaa ttgctcagga tcgctccccc accattcgcg
     6421 cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg attctggtgg
     6481 tgtttttatt cctgcgctcg ggtcgcgcaa ctattattcc cgccgttgcg gtgccggttt
     6541 cgctgattgg tacgtttgcg gcgatgtacc tgtgcggttt cagtctcaat aatctttcgt
     6601 taatggcgct caccatcgct actggtttcg tggtggatga cgccatcgtg gtgctggaaa
     6661 acattgcccg tcatctggaa gcgggaatga aaccgttgca agccgcactg caaggtactc
     6721 gcgaagtcgg ttttacggtg ctgtcgatga gtctgtcact ggtggcggtg ttcctgccgc
     6781 tgctgttgat gggcggattg ccgggccgac tgttacgcga atttgccgtg acgctttctg
     6841 tcgccattgg tatatcgttg ctggtttctc tgacattaac gccaatgatg tgtggctgga
     6901 tgctgaaagc cagcaagccg cgcgagcaaa agcgactgcg tggttttggt cgcatgttgg
     6961 tagccctgca acaaggctac ggcaagtcac tgaaatgggt gctcaatcat acccgtctgg
     7021 tgggcgtggt gctgcttggc accattgcgc tgaatatctg gctgtatatc tcgatcccga
     7081 aaaccttctt cccggagcag gacactggcg tgttgatggg cgggattcag gcggatcaga
     7141 gtatttcgtt tcaggcgatg cgcggtaagt tgcaggattt catgaaaatt atccgtgacg
     7201 atccggcagt ggataatgtc accggcttta caggcggttc gcgagtgaac agcgggatga
     7261 tgtttatcac cctcaagcca cgcgacgaac gcagcgaaac ggcgcagcaa attatcgacc
     7321 gtctgcgcgt aaaactggcg aaagaaccgg gggcgaatct gttcctgatg gcggtacagg
     7381 atattcgcgt tggtgggcgt cagtcgaacg ccagctacca gtacacgttg ttatccgacg
     7441 acctggcggc actgcgcgaa tgggagccga aaatccgcaa aaaactggcg acgttgccgg
     7501 aactggcgga cgtgaactcc gatcagcagg ataacggcgc ggagatgaat ctggtttacg
     7561 accgcgacac catggcacgg ctgggaatcg acgtacaagc cgccaacagt ctgttaaata
     7621 acgccttcgg tcagcggcaa atctcgacca tttaccagcc gatgaaccag tataaagtgg
     7681 tgatggaagt ggatccgcgc tatacccagg acatcagtgc gctggaaaaa atgttcgtta
     7741 tcaataacga aggcaaagcg atcccgctgt cgtatttcgc taaatggcaa ccggcgaatg
     7801 ccccactatc ggtgaatcat cagggattat cggcggcctc gaccatttcg tttaacctgc
     7861 cgaccggaaa atcgctctcg gacgccagtg cggcgatcga tcgcgcaatg acccagcttg
     7921 gtgtgccttc gacggtgcgc ggcagttttg ccggcacggc gcaggtgttc caggagacga
     7981 tgaactcgca ggtgatcctg attatcgccg ccatcgccac ggtgtatatc gtgctgggta
     8041 tcctttacga gagttacgta catccgctga cgattctctc caccctgccc tcggcgggcg
     8101 ttggagcgct gttggcgctg gagctgttca atgccccgtt cagcctaatc gccctgatag
     8161 ggatcatgct attaatcggc attgtgaaga aaaacgccat tatgatggtc gattttgcgc
     8221 ttgaagccca acggcacggt aacctgacgc cgcaggaagc tattttccag gcctgtctgc
     8281 tgcgttttcg cccgattatg atgactaccc tggcggcgct gtttggtgcg ctgccgctgg
     8341 tattgtcggg cggcgacgga tcggagttgc gccaacccct ggggatcacc attgtcggcg
     8401 gtctggtaat gagccagctc ctgacgctgt ataccacgcc ggtggtgtat ctctttttcg
     8461 accgtctgcg gctgcgtttt tcgcgtaaac ctaaacaaac ggtaaccgag taaatgacag
     8521 atcttcccga cagcacccgc tggcaattgt ggattgtggc tttcggcttc tttatgcagt
     8581 cgctggacac caccatcgta aacaccgccc ttccctcaat ggcgcaaagc ctcggggaaa
     8641 gtccgttgca tatgcacatg gtcattgtct cttatgtgct gaccgtggcg gtgatgctgc
     8701 ccgccagcgg ctggctggcg gacaaagtcg gcgtgcgcaa tattttcttt accgccatcg
     8761 tgctgtttac tctcggttca ctgttttgcg cgctttccgg cacgctgaac gaactgttgc
     8821 tggcacgcgc gttacagggc gttggcggcg cgatgatggt gccggtcggc agattgacgg
     8881 tgatgaaaat cgtaccgcgc gagcaatata tggcggcgat gacctttgtc acgttacccg
     8941 gtcaggtcgg tccgctgctc ggtccggcgc tcggcggtct gctggtggag tacgcgtcgt
     9001 gtcactggat ctttttgatc aacattccgg tggggattat cggtgcgatc gccacattgc
     9061 tgttaatgcc gaactacacc atgcagacgc ggcgctttga tctttccggc tttttattgc
     9121 tggcggttgg catggcggta ttaaccctgg cgctggacgg cagtaaaggt acaggtttat
     9181 cgccgctggc gattgctggc ctggtcgcag ttggtgtggt ggcactggtg ctttatctgc
     9241 tgcacgccag aaataacaac cgcgctcttt tcagtctgaa actgttccgc actcgtacct
     9301 tttcgctggg cctggcgggg agctttgccg gacgtattgg cagtggcatg ttgcccttta
     9361 ttacaccggt tttcctgcaa attggcctcg gtttctcgcc gtttcatgcc ggactgatga
     9421 tgatcccgat ggtgcttggc agcatgggaa tgaagcgaat tgttgtacag gtggtgaatc
     9481 gctttggtta tcgtcgggta ctggtagcga ccacgctggg tctgtcgctg gtcaccctgt
     9541 tgtttatgac taccgctctg ctgggctggt actacgtttt gccgttcgtc ctgtttttac
     9601 aagggatggt caactcgacg cgtttctcct ccatgaacac cctgacgctg aaagatctcc
     9661 cggacaatct ggcgagcagc ggaaacagcc tgctgtcgat gattatgcaa ttgtcgatga
     9721 gtatcggcgt caccatcgcc gggctgttgc tgggactttt tggttcgcag catgtcagcg
     9781 tcgacagcgg caccacacaa accgtcttta tgtacacctg gcttagcatg gcgtttatca
     9841 tcgcccttcc ggcgttcatc tttgccagag tgccgaacga tacgcatcaa aatgtagcta
     9901 tttcgcggcg aaaaaggagc gcgcaatgaa gttctggcga cctggtatta ccggcaaact
     9961 gtttctggcg attttcgcca cctgcattgt cttgctgagc agtatgcact gggcggtgcg
    10021 tatcagtttt gagcgtggct ttattgatta catcaagcat ggtaatgaac agcgattaca
    10081 actgttaagt gatgcgcttg gcgagcagta tgcgcagcat ggcaactggc gcttcctgcg
    10141 caacaatgat cgctttgtct ttcagatcct gcgttcattt gaacacgata attcggaaga
    10201 taaacccggc ccgggtatgc caccgcacgg ctggcgtacc cagttctggg tggttgatca
    10261 aaacaacaaa gtgctggttg gtccgcgagc gccgattcca cctgacggta cacggcgacc
    10321 cattctggtc aacggtgcgg aagtgggcgc ggtgatctcc tcccccgttg agcggttaac
    10381 gcgcaatact gatatcaatt tcgataaaca acagcggcaa accagctggt tgattgtcgc
    10441 tctggcaacg ttactcgcgg cgctcgccac ctttctgctg gcgcgtggtc tgttggcccc
    10501 ggtcaaacgg ctggtggacg gcacgcacaa actggcggcg ggcgatttca ctacccgcgt
    10561 aacgcccacc agtgaagatg aactaggcaa actggcgcaa gacttcaacc agctcgccag
    10621 cacgctggag aaaaaccagc aaatgcggcg cgattttatg gccgatattt ctcacgaact
    10681 gcgtacgcca ttagcggtgc tgcgcggtga actggaagcc attcaggatg gcgtgcgtaa
    10741 attcacgccg gagacggtgg cgtctttaca ggcggaggtc ggtacactga ccaaactggt
    10801 tgacgatctc catcagttgt cgatgtctga tgaaggcgct ctcgcctatc aaaaagcacc
    10861 ggtagatttg atcccactgc tggaagtggc gggcggcgca tttcgcgaac gattcgccag
    10921 ccgtggcctg aaactgcaat tttccctgcc agacagtatc accgtatttg gcgatcgcga
    10981 ccgtttaatg cagttattca ataacttact tgaaaacagc ctgcgctaca ctgacagcgg
    11041 cggcagcctg aaaatctctg ccgagcagca cgacaaaacg gtgcgcctga cctttgccga
    11101 cagcgcgccg ggcgtcagtg acgatcagct acaaaaattg tttgaacgtt tttatcgcac
    11161 cgaaggttcc cggaaccgcg ccagcggcgg ttccgggctg gggctggcga tttgcctgaa
    11221 cattgttgaa gcacataatg gccgcattat tgctgcccat tcgccttttg gcggggtaag
    11281 cattacagta gagttaccgc tggaacggga tttacagaga gaagtatgac cgagttacca
    11341 atcgacgaaa acacaccgcg tattttgatc gtggaagatg aaccgaagct ggggcagttg
    11401 ctcattgatt atctgcgtgc tgcgagctat gcgccgacgc ttatcagcca cggcgatcag
    11461 gtactgccgt atgtgcgcca gacaccaccg gatctgatcc tgttggatct gatgctccct
    11521 ggcaccgatg gcttgacgct gtgccgggaa attcgtcgtt tttctgacat tcctatcgtg
    11581 atggtgacag caaaaatcga agagatcgac cgcctgctgg ggctggagat tggcgcagat
    11641 gactacatct gcaagccgta cagcccacgg gaagtggtag cgcgcgtcaa aaccattttg
    11701 cgccgttgca aaccgcagcg cgaattgcag caacaggatg ctgaaagtcc gttgattatc
    11761 gacgaaggtc gttttcaggc ttcatggcgc ggtaaaatgc ttgacctgac gcctgcggaa
    11821 tttcgtctgc tgaaaacgct ctctcacgaa ccaggaaaag tgttctcccg cgagcaattg
    11881 ctcaatcatc tttatgacga ctaccgcgta gtaaccgacc gcaccatcga cagccacatt
    11941 aaaaacctgc gccgcaagct ggaatctctc gacgccgaac agtcatttat ccgcgccgtt
    12001 tatggcgtcg gttaccgctg ggaagccgac gcctgccgca tcgtttagtt ttagcgacat
    12061 tattttgtta gccggagatg gcgttcagac ccgatccggc aacattatcc cacgcatggt
    12121 cagcaaactg acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat
    12181 cggtaaacag cacctttaga tgctgttttc gatacactat ttcaatcaag gagtcattat
    12241 ggctggttgg tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc
    12301 gggcaatggt gagactatcc tcaccagcga gctgtatacc tcaaaagcct ctgcggaaaa
    12361 gggcatcgcg tcggtgcgta gcaacagccc gcaagaagaa cgctatgaga aaaaaacggc
    12421 aagtaacggc aaattctatt tcaatctgaa agccgctaat catcaaatta tcggctccag
    12481 ccagatgtac gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg
    12541 cacaagccag acggtgaaag acaatacgta attacgatgc cgggcgtggg ttgttggcgt
    12601 ccggcgcttt accagatccc cccacagcgc tacaatgccc gcccttaaag tgggggcact
    12661 cccctaaccg cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg
    12721 tttaaaccgg aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct
    12781 tatggcgcag atgctgttta tgcgggccag ccgcgttact ccctgcgtgt gcgcaacaac
    12841 gaattcaacc acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag
    12901 ttttatgtcg tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac
    12961 ctgaaaccgg tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt
    13021 atgctggtgc gtgagcactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc
    13081 gtaaactggg cgacggtgaa attctggcag caaatgggcc tgacccgcgt gatcctctct
    13141 cgcgagctgt cgctggaaga gattgaagag atccgcaatc aggtgccgga tatggagatc
    13201 gagatcttcg ttcacggtgc gctgtgcatg gcctactccg gtcgctgcct gctctctggc
    13261 tatatcaaca agcgcgatcc gaaccagggt acctgcacca acgcctgccg ctgggagtac
    13321 aacgtccagg aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatt
    13381 ccggtgcaaa atgttgagcc gacgctgggt atcggcgcgc caaccgacaa agtgtttatg
    13441 atcgaagaag cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact
    13501 tacatcatga actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg
    13561 ggcgtgcatt cgctgaaaat cgaaggccgt actaaatctt tctactattg cgcacgcacc
    13621 gcacaggttt accgtaaagc tatcgatgac gccgctgcgg gcaaaccgtt cgataccagc
    13681 ctgctggaaa ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt
    13741 catactcacg acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag
    13801 tttgttggtg agtttaccgg tgagcgcaag ggggacctcg cggcggtagc ggtgaaaaat
    13861 aaattctccg ttggcgacag ccttgagctg atgacgccgc aaggcaacat taactttacc
    13921 cttgagcaca tggaaaacgc caaaggtgaa gcgatgccgg tcgcaccagg cgatggttat
    13981 actgtgtggc tcccggtccc gcaggatctt gagctcaatt acgctctgct gatgcgtaat
    14041 ttctccgggg aaaccacgcg taacccacac ggtaagtgat taatttcgat tatttttccc
    14101 gtatggaaaa ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt
    14161 ttcgctgaaa aacataaccc ataaaatgct agctgtacca ggaaccacct ccttagcctg
    14221 tgtaatctcc cttacacggg cttatttttt tatgcataag ccctatctct ggtaaccgtc
    14281 ttccattgac cacatcgata gaatcctcct tcatagcacg atgcctttca cttatcggca
    14341 tcgtgctccc acaggttccg gctacgcaca gccagaacgc gcatatttga cgcttaccaa
    14401 aaaatattct cactctccac atttgaatgt cagacgagcg acaccatgta atcctacacc
    14461 ttctgtcttc agctcaacta tttgcatttt tttgccctga gtaacacaga aatgagttgc
    14521 atcatttttt actatatttt ctgcaccaga tattctaccc ctggctaaag aagcttcggc
    14581 ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg caagaccaaa
    14641 tttaccgata cttgtctcat cattatgcac agcacaacca gacataataa aaatactaat
    14701 taatgatata gcagctatct ttttcatctc accttccccc attaaatacc aacgacactc
    14761 tctagtgttt aaatataata atggcatgat tattataatt gaataggatt ataataaatg
    14821 ttctgtacaa catttcctac ataagtagga attacggaca ttgaggccct tcagggtaac
    14881 tccatggggg ctttaatata ttatattgaa gatgccactg tttagttgaa tattaggtat
    14941 atgctctttt ttgaaattta tcggtggcag ccgttagtat tcgctgtccc cattgcaagc
    15001 tcctggtggt aaccactgaa tcctccatac ttgaactgac tttttatcct ccgactttca
    15061 tcctgttctg actccacctt ttgttttctg ctctacacta tctacagacc aatcataaag
    15121 gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg caaaagtact
    15181 gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat gacgatccat
    15241 gtgcgggtca cctgggagaa aggcgatgcc gcacgatatg tagaggaggc ccggaagttg
    15301 ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt ttctacggcg
    15361 ttgattcagt gtgaggggga tgacataccc gcgctgggaa ttttgccatt aggaaccgcc
    15421 aatgattttg ccaccagtgt agggattcct gaggtactgg ataaggcgct gaaactggca
    15481 attgccggta acgccattgc gatagatatg gcgcaggtca acaaacaaac ctgttttatt
    15541 aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga aaaattaaaa
    15601 gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga tactctgcaa
    15661 ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc cctggtcatt
    15721 agtattggta acgggcgtca ggccggtggc ggtcagcaat tgtgtccgaa cgcgttaatt
    15781 aacgatggct tgctgcaact gcgcattttt accggcgatg aaattcttcc agctctcgta
    15841 tcaaccttaa aatctgacga agataacccg aatattatcg aaggcgcttc gtcgtggttt
    15901 gatatacaag ccccacatga aatcactttt aatcttgatg gcgaaccgtt gagcggacaa
    15961 aactttcata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc ggattgtcca
    16021 ttattgcgtt aatcaaacta ttctctcttg aaccagggag aatccgtaat gccggatgcg
    16081 gtgtaaatac cttatccggc atacagaaca atactacacc gtaattaatt tcacccgtgt
    16141 cttctgcaat gccagtttat ccacatcaga cacagcatca tcggtaataa taatgtcaat
    16201 tttttcgatt ggcagtacct gattaaaacc gcgacggtta aatttcgatg aatcgagaac
    16261 ggcaatcact ttattagcgg ctgtgaccat cgcaccgcta acggaataac cctcattaaa
    16321 ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta aatcggcatt
    16381 aatatcctgc agggaacgtt cagcaataga tccatgcata gatcgcgttt tatggcggac
    16441 cgtgccaccg cagacgacca gagtaatgtc tttattttcg gaaagggcaa acgcagccgg
    16501 gagactgttg gtaatcaccg tgatattttt ggcggtcatt aatccttcgg cgataagcat
    16561 tgtggtactt ccgctgtcga gaataacggt catcccttca tggatcattt tgactgccgc
    16621 ctgcgctatt ctgtttttcg gcgcgctggc gagctgaaat cgctctttaa acccgacttc
    16681 ctgggtctcg gtttcactat taccagacat tattttcgcc gcaccgccat gaaagcgcgt
    16741 aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt ccgaggcagc
    16801 aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta attggatgat
    16861 cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg tggctagtgt
    16921 aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc agcatcacgc
    16981 tggcccgcgg tttcatggaa tgagcaacac tttgcccggc atggcattac gagcgatgtc
    17041 acgcaccgcc tgggtgaagc tttcaaagct tccacggtga gcgattaatg gctccaggct
    17101 taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg gccaagggct
    17161 ggaatagttc atccaactgc cgataaccgt cagctcttta cgcaatattt tgccaaacgt
    17221 tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt gggcatgagg
    17281 accggcaatc tctaccgcca gttcgacagt ttgcggtacg ccagccgtct cgaggataag
    17341 ctgattaaaa cgcag
//
LOCUS       DAJTNA010000081        16613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTNA010000081 DAJTNA010000000
VERSION     DAJTNA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16613
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.082"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            271..2118
                     /gene="ldtD"
                     /locus_tag="OO161_002244"
     CDS             271..2118
                     /gene="ldtD"
                     /locus_tag="OO161_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5463298.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPTHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2299..2847
                     /locus_tag="OO161_002245"
     CDS             2299..2847
                     /locus_tag="OO161_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5463299.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2874..3521
                     /gene="gloC"
                     /locus_tag="OO161_002246"
     CDS             2874..3521
                     /gene="gloC"
                     /locus_tag="OO161_002246"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5463300.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3743..4933)
                     /gene="aspC"
                     /locus_tag="OO161_002247"
     CDS             complement(3743..4933)
                     /gene="aspC"
                     /locus_tag="OO161_002247"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5463301.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5118..6206)
                     /gene="ompF"
                     /locus_tag="OO161_002248"
     CDS             complement(5118..6206)
                     /gene="ompF"
                     /locus_tag="OO161_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5463302.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6809..8209)
                     /gene="asnS"
                     /locus_tag="OO161_002249"
     CDS             complement(6809..8209)
                     /gene="asnS"
                     /locus_tag="OO161_002249"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5463303.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8378..9580)
                     /gene="pncB"
                     /locus_tag="OO161_002250"
     CDS             complement(8378..9580)
                     /gene="pncB"
                     /locus_tag="OO161_002250"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5463304.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TTGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9846..12458
                     /gene="pepN"
                     /locus_tag="OO161_002251"
     CDS             9846..12458
                     /gene="pepN"
                     /locus_tag="OO161_002251"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5463305.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12501..13252)
                     /gene="ssuB"
                     /locus_tag="OO161_002252"
                     /pseudo
     CDS             complement(12501..13252)
                     /gene="ssuB"
                     /locus_tag="OO161_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13249..14040)
                     /gene="ssuC"
                     /locus_tag="OO161_002253"
                     /pseudo
     CDS             complement(13249..14040)
                     /gene="ssuC"
                     /locus_tag="OO161_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(14051..15196)
                     /gene="ssuD"
                     /locus_tag="OO161_002254"
                     /pseudo
     CDS             complement(14051..15196)
                     /gene="ssuD"
                     /locus_tag="OO161_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15193..16151)
                     /gene="ssuA"
                     /locus_tag="OO161_002255"
                     /pseudo
     CDS             complement(15193..16151)
                     /gene="ssuA"
                     /locus_tag="OO161_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
     gene            complement(16144..>16613)
                     /gene="ssuE"
                     /locus_tag="OO161_002256"
     CDS             complement(16144..>16613)
                     /gene="ssuE"
                     /locus_tag="OO161_002256"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5463306.1"
                     /translation="WNLQNFAPEDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAA
                     YSGALKTLLDLLPERALQGKVVLPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVF
                     ADDSQVIDYHHRPQFTPNLQTRLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4140 a         4315 c         4253 g         3905 t
ORIGIN      
        1 ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc aactgatcct
       61 taactgatcg gcattacaca taacggcgcg cttttcttta aacttcttta cattaggtta
      121 tgtaaaaacg tatcggcgtt tatatactga agataagcct gatgagtaac aggcttgctc
      181 gtcatacttt cgtgagtatt ggcgttgtac aggcaagtcg taaaataaca gcctggctat
      241 tcagagtatg ataaaaacag ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg
      301 ctgtcggcaa tcagtttgtg cctggccgta acattcgctc cactgttcaa tgcgcaggcc
      361 gatgagcctg aagtaatccc tggcgacagc ccggtggctg tcagtgaaca gggcgaggca
      421 ctgccgcagg cgcaagccac ggcaataatg gcggggatcc agccattgcc tgaaggtgcg
      481 gcagaaaaag cccgcacgca aatcgaatct caattacccg caggttacaa gccggtttat
      541 cttaaccagc ttcaactgtt gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt
      601 gatgctgtta aagccttcca gcaacagctg gcagaggtgg cgattgccgg tttccagccg
      661 cagtttaata aatgggtaga gttactgacc gatcctggtg ttaacgggat ggcacgcgac
      721 gtagtgctct ctgatgcgat gatgggctat ctccatttca ttgcaaatat tccggtcaaa
      781 ggcactcgct ggctatatag cagtaaacct tatgcacttg caacgccgcc gctctcggtg
      841 attaaccaat ggcagctggc gctggataaa ggtcaattgc ctacgtttgt tgcaggactg
      901 gcaccgcagc atccgcaata tgcggcgatg catgaatcgt tactggcctt actcagtgac
      961 accaaaccgt ggccccaact gaccggcaaa gcaacgttgc gcccagggca gtggagtaac
     1021 gacgtaccgg cgttgcgcga aatattgcaa cgcacaggca tgttggacgg ggggccgaaa
     1081 attactctac ctggcgatga cacgccaact gacgcggtag tcagcccatc cgctgttact
     1141 gttgaaacag cagaaactaa gccgatggac aagcaaacga cgtctcgtag taaacctgcg
     1201 cctgccgttc gcgccgccta cgataatgaa ctggtggaag ccgttaaacg ttttcaggca
     1261 tggcaaggat tgggggcaga tggtgctatt ggcccggcaa cacgtgactg gttaaacgta
     1321 acgcccgccc agcgtgctgg cgtgttggct ctcaacatcc agcgattgcg cttgctgcca
     1381 acagagcttt ctaccgggat catggttaac attccggcct attcgctggt ctactatcag
     1441 aacggcaatc aggtgctgga ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg
     1501 atgatgagca gtgcccttaa caacgtagtg gtaaacccgc cgtggaacgt acctccaact
     1561 ctggcacgca aagatattct gccaaaagtg cgcaacgatc cgggatatct cgaaagccat
     1621 ggctatacgg tgatgcgtgg ctggaacagc agagaagcga ttgacccatg gcaggttgac
     1681 tggtctacaa tcacggcctc gaatttaccg ttccgcttcc agcaggctcc aggcccacgg
     1741 aactcgctgg ggcgctataa attcaatatg ccgagttcag aggccattta tttgcatgac
     1801 acgccgaccc acaatctgtt caagcgtgat acacgcgcat tgagctcagg ctgtgtacga
     1861 gtgaataaag cttccgatct ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa
     1921 cgtatttctg atgcgctgaa gcagggtgat acacgttacg tcaatattcg gcagtcgatt
     1981 ccggtgaatc tctactacct gacggccttt gttggtgcag atggtcgtac ccagtatcgt
     2041 acagatattt acaattatga tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg
     2101 gaacaattaa tcaggtaaat gaagtagttc ggggaaatca gttgtcgtaa taattagcat
     2161 gattgggggc gattctctgc agcccccgtc actgctgggg ttgagtcatc ttgacgtctg
     2221 ctttacgggc ggttaaggtg cctcttgtgc gccagaagtg catataaacg ataacattga
     2281 cctgtagact tgattatcat ggacaaattc gacgctaatc gccgcaaatt gctggcgctt
     2341 ggtggcgttg cactcggtgc cgccatcctg ccgacccctg cgtttgcaac actctctacc
     2401 ccacgcccgc gcattttgac actcaataat cttcataccg gagagtcaat caaagcggag
     2461 tttttcgatg gcagaggcta tattcaggaa gaattggcaa aacttaacca ttttttccgc
     2521 gattaccgcg cgaacaaaat aaagtccatc gacccaggat tattcgacca gttgtatcgc
     2581 ctgcaagggt tgttaggcac gcgcaaaccg gtgcaactca tttccggtta tcgttccatt
     2641 gataccaaca atgaactacg cgcccgcagc cgtggagtag cgaagaaaag ctatcacact
     2701 aaaggccagg cgatggattt ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc
     2761 gcgttatcta tgcgcgcagg tggtgtagga tattacccac gtagtaactt tgtgcatatt
     2821 gataccgggc cagcacggca ctggtagcaa tcgcttaacg aaacaggggc agtatgaact
     2881 atcgtattat tccggtcacc gcattctctc agaactgttc attaatctgg tgtgaacaaa
     2941 cccgtctggc cgcactggtc gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg
     3001 atgacagcgg cctgacactg atgcagatcc tgctgacgca tggtcatctg gaccacgttg
     3061 gcgcagcggc ggaactggcg caacattacg gcgtgccggt ttttggcccg gaaaaagaag
     3121 atgagttctg gctgcaaggc ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc
     3181 agccgctgac gccagatcgt tggctgaacg aaggcgatac catcagcata ggaaatgtga
     3241 ctttacaggt gttacattgc cctgggcata cgccgggtca tgtcgtgttt tttgatgatc
     3301 gggcaaagct gctgatttct ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact
     3361 tcccgcgtgg cgatcataat caactgattt cttcaatcaa agataaattg ctgccactgg
     3421 gggatgacgt gacatttatt ccgggtcacg gaccattatc cacacttggt tatgaacgcc
     3481 tgcataatcc cttcctgcaa gacgaaatgc ccgtctggta aggcacataa aaaagcccgc
     3541 ttttaatgcg ggcctggatt tctggcaaag tgcgctttgt ttatgccgga tgcggcacga
     3601 gcgccttatc cggcctacaa aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc
     3661 tgataagcgt agcgcatcag gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag
     3721 cgggcttcat tgtttttaat gcttacagca ctgccacaat cgcttcgcac agcggagcca
     3781 tgttatctgg tgtcatcccg gccacattta cgcgaccaga agcaaccgca tatacgccaa
     3841 actcttcgcg cagacgcagc acttgttctt ttgtcaggcc actgaaggag aacatgccgt
     3901 tctgtttgat gataaagctg aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga
     3961 acaactgacg catacgctga atacgctggc gcatatcagt cagctcttgt tcccaaatcg
     4021 cacgtaacgc atcgttgctc aggatggtgg caacaacaga agcgccgtgt gctggtgggt
     4081 tagagtagtt agcgcgaatc gccgctttca tttggctgaa tgcgcgatca acggtttcac
     4141 tgtcggcagc aaccagagta caagcgccaa cacgctcgtt gtacaggcca aagtttttag
     4201 agtaggaact ggcaacaatc agctctttat gcatagccgc gaaagcgcgc agtccttcag
     4261 catcttcttc cagaccacgg gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc
     4321 ctttctcaac ggagagttgt gccagtgtct gccattgttc cagcgtaggg tcgataccgg
     4381 ttgggttatg gcagcagcca tggaacagca ctacgtcgcc agcctgagct tcattcaggc
     4441 tgttaatcag tgcatcgaag tcaagagtgt gattttccgc atcataataa gcgtattcac
     4501 gaacttccag acctgcagag ttaaagacgc tcttatggtt cggccagctt gggttgctca
     4561 cccacactcg cttaacgctg gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc
     4621 cagtgccccc cggagtctgt gccgtgcgag cacgtttgtc attgatcagg gcgctacctt
     4681 taccaaacag cagttcctga gtgcagcgac caaattcagg gatgccgtca atgccgaggt
     4741 aatttttggt ggtttcattt tcgagcagat actgttcagc ctttttcacg ctggtcagta
     4801 ccggggtttt gcccgtctca tctttataga caccaatccc gaggttaatt ttgccgggac
     4861 gttcatcggc acgaaacaga tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa
     4921 tgttctcaaa catgacgagg ttccattatg gttacagaag ggaagtccgc tatcagggta
     4981 acgggagatt tacaaaattc caactattac tgatgaaaac gcaggctgtt tttgcaagac
     5041 gtgagattgc tctggaaggt ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt
     5101 aacaaagagg tgtgctatta gaactggtaa acgataccca cagcaacggt gtcgtctgaa
     5161 cctacgccca gtttgttgtc agaatcgatc tggttgatga tgtagtcaac ataggtggac
     5221 atgtttttgt tgaagtagta ggttgcgccc acttcaaagt agttcaccag atcaacatca
     5281 ccgatacctt ctacgtcttt cgctttagat ttggtgtaag cgatggacgg acgcagaccg
     5341 aaatcgaact ggtattgcgc aactaacaga acgtcttgcg ttttgttggc gaagccgctg
     5401 gtgtttgtaa atttattagt gatcggcgta gcgttacggg tttcaccgta gttcgctgcc
     5461 aggtagatgt tgttcgcgtc gtatttcaga ccagtagccc actgttcagc ttttttaccg
     5521 ttgccaagag gttgagcttc ttgcaggttg gtacggtcag ctgcaccata agcaccaacg
     5581 ataccaaagc cttcgtattc gtagctgata gaaccgccaa caccgtcgcc gttagaacgg
     5641 cgtgcagtgt cacgctcgtt tttacccagg tactgaacag cgaagttcag gccatcaacc
     5701 agaccaaaga agttggagtt acgataggta gcaacgccgc caacacgacc aacgaagaag
     5761 tcatcgctgt atgcagtatc accaccaaat tctggcagca tatcggtgta acccagtgca
     5821 tcataaacca caccgtagtt acggccgtaa tcgaaagaac caacgtcagc gtatttaaga
     5881 cccgcgaatg ccagacgcgt tttgttacca gtttgagcgt cagcgccttc agagttgtta
     5941 ccttggaagt tatattccca ctgaccataa ccggtcagat cggaattgat ttgagtttcc
     6001 cctttaaaac caagacgggc ataggtcatg tcgccattgc caccgtaact gttttcaccg
     6061 ttacccttgg aaaaataatg cagaccaaca gctttaccgt acagatctac tttgttgcca
     6121 tctttgttat agatttctgc agcgtttgca gtacctgcta ctaacagagc agggacgatc
     6181 actgccagaa tattgcgctt catcattatt tattaccctc atggtttttt ttatgacacc
     6241 tgccactgcc gtcaataagt tctgtcaata aaaatttacg gaactattga tgagagtttg
     6301 gtgtctttat gtgtctgcag gcatctttcc attcaaacta acgtttcgct accgtgaaag
     6361 tgctgcaaag ataaagattt agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt
     6421 aaggaacttt gtgaacaccg tcatatttcc atagaaacgt gatgatattt acagcaattt
     6481 ttatctattt atatgattta cttatattta aattaactaa gcggaaattt tgtttctgat
     6541 ggaaacttta tcgacctggc acaaaatcct ctttcagaca tccagaatgc caaaaaatag
     6601 tatgaaaata gtgctatcag ctaagaaaaa actaatccgc aataagaatg cgggttattt
     6661 ttttgtgcgt aaatgttgca atgtaatatt ccgtaacagg atgatcgtta tggcggggtt
     6721 gcaaatagat tgcttgactt aacaaacaga taacttgaca gaaaggataa aaaagagcca
     6781 gcaggcgctg gctctttggg gaagaaaatc agaagctggc gttgcgcgga gtacgtggga
     6841 acggaatcac atcacgcacg ttttgcacgc cagttacgta agcaatcagg cgttcaaaac
     6901 caagaccgaa acctgaatgc ggaacagtac cgtagcgacg cagatcgcga taccaccagt
     6961 aatcttcttt attcaggccc atttccagca tacgctcgtc cagaacgtcc agacgttctt
     7021 cacgctggga gccaccaatg atctcaccga tgcccggagc cagaacgtcc atagccgcaa
     7081 cggttttacc gtcttcgttg aggcgcatat agaacgcttt aatatctttc ggatagtttt
     7141 taaccactac cggtgcttta aagtgttctt ccgccagata acgctcatgc tcagaagaga
     7201 gatcgacgcc ccagtaaacc gggttttcaa acttcctgcc gcagttttcg agaatggtca
     7261 ctgcgtcggt gtaatccacc tgcgcgaaat cggcctcaat gaagcgttcc agacgtgaaa
     7321 cggcatcttt atctacgcgt tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa
     7381 ccgctttgaa gacatatttc agcatggctt cagccagacc cgcgatatcg ttcaggttag
     7441 caaacgccac ttccggctcc agcatccaga attccgccag gtgacggctg gtgttggagt
     7501 tttcagcacg gaaagtcggg ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt
     7561 cgccgttcaa ctggccagat acggtcagga aagactcttt accaaagaag tctttgtcga
     7621 aatccacttt gccctgatcg ttacgcggca ggttttccag atccagcgta gaaacgcgga
     7681 acatttcgcc tgcaccttcg gtatcagatg cggtaatcag tggcgttgaa acccagaaga
     7741 atccctgctc gttaaagaag cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga
     7801 cggcaccaat caggtttgtg cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc
     7861 tgtggcgttt tgccgccatc gggtaagtgt ctggatcttc aacccaacca gcaacttcaa
     7921 ctttgctggc ctgaatttca aattgttgcc cctggcccgg cgacgccacg actttacccg
     7981 tcacaatgac cgagcagccg gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag
     8041 aattattgat gacagcctgt acaggatcaa agcaggaacc gtcataaacg gcgaggaagg
     8101 agatgccagc ttttgaatct cggcgggtac gtacccatcc gcgtacggtg acttcgctgt
     8161 caacggctac gcggccctgg agtacgtcgg ctacaggcac aacgctcata atattctctc
     8221 tgttaatagt cggaaaaaat aaacacttgt ccacccgtaa atggggggat acctatgtta
     8281 cctggcatct gcaatcagac aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa
     8341 aggtaaaaaa agggagccga ttagctccct gatgatatta actggctttt ttaatatgcg
     8401 gaaggtcgaa cgctttgcgc agcgcccgaa caaacgcttt atcatggcag atagttttgc
     8461 cagggctgtc agaaagtttc gccaccggtt taccgttaca ctctaccaac ttaatcacaa
     8521 tattcagggg ttttacctgg gggatatcgc aggtcaggcg agtcccaata ccaaaactta
     8581 attgcacgcg ggaagagaag tggcggtata gctcaaccgc tttgcgtaaa tccagattgt
     8641 cagagaaaac cagcgtttta ctctgtggat caattcccag ctttttataa tgtgcaatgg
     8701 ctttttcacc ccattcaacc gggtcgccag agtcatgacg caggccctga taccgactgg
     8761 cgaactcgac accgaaatca cgcaggaaag catccatagt gatgcagtcg gttaatgcaa
     8821 tgccaagttg gtcgggatat tcttccagcc aggcagcaag tgcagctcgc tggctgttgg
     8881 ctaggtccgg gctgatttgc tgatgtgcct ggaaccattc gtgcgcctgt gttcccatcg
     8941 gcgtgaggga aagccgacgc gccagatcgt agttgctggt gcccacgaac caggattcct
     9001 gttgcagacg cttaacgatg gtttcttgta cttcgcgaga aaaacggcga cgggtgccaa
     9061 aatccatcag atggaagcgc gacatatcaa gaccggtggt taacgccgag aagtcgacta
     9121 atttgctttc cagcgtgtcg agggcttgcg caacgtcggc ctgcggtgag cgatagcgat
     9181 gtaccatttc actgataacc gccagcaaag gaacttccca gaggatgact tcacgccacg
     9241 gaccgcttaa gcgaatatcc agcttgccat tatcgttgga cacggtgact tgttccgggt
     9301 taaagcggaa ttcgcgtaac cagttaagat agtcggcctt aaagaaaggc agggcagaga
     9361 gccactgata ttcatcatcc tgcaggcgca ggtgctgcat cgcctgaatc tgttcacgaa
     9421 tagcatcggc ataaataccc agcagatcgt cacctcggca acgaaactcc gccgcgacat
     9481 gcacatcgta atagtgatga aacacggctt gctgcatatg caacttataa gcatctgtat
     9541 ccagcaacga gtgcagaaca ggagaagcga attgtgtcat aggtgcgcag tagcatcctc
     9601 gtacaggagc gtttagtaca ataaacatct tcaggaactg ctggagtata ccttgtttcg
     9661 caatttattg aaccccgatc acaccatatg ccacctttct ggtcgatggc attacgcggt
     9721 gtatgttata aaaatgtagc aataaaggcg tttgtacctg aaaagatgaa gattctgcat
     9781 agcgcgattt acgcaacagg aatagactga acaccagact ctataaaaga tgctaaaggt
     9841 tatttatgac tcaacagcca caagccaaat accgtcacga ttatcgtgcg ccggattacc
     9901 agattactga tattgacttg acctttgacc tcgacgcgca aaagacggtc gttaccgcgg
     9961 tcagccaggc tgtccgtcat ggtgcatcag atgctccgct tcgtctcaac ggcgaagacc
    10021 tcaaactggt ttctgttcat attaatgatg agccgtggac cgcctggaaa gaagaagagg
    10081 gcgcactggt catcagtaat ttgccggagc attttacgct taagatcgtt aatgaaataa
    10141 gcccggcggc gaataccgcg ctggaagggc tttatcagtc aggcgatgcg ctttgcaccc
    10201 agtgtgaagc cgaaggtttc cgccatatta cgtattatct cgaccgcccg gacgtgctgg
    10261 cgcgttttac caccaaaatt attgccgata aaaccaaata tcccttcctg ctttccaacg
    10321 gtaaccgcgt tgcgcaaggt gaactggaaa acggacgcca ttgggtacag tggcaggacc
    10381 cgttcccgaa accgtgctac ctgtttgcgc tggtggcagg cgactttgat gtactgcgcg
    10441 acacctttac cacgcgttct ggtcgcgaag tggcgctgga gctgtacgtc gatcgcggca
    10501 accttgatcg cgcgccgtgg gcgatgacct cgctgaaaaa ctcaatgaaa tgggatgaag
    10561 aacgcttcgg cctggagtat gacctcgaca tctatatgat cgtcgcggtg gatttcttca
    10621 atatgggcgc aatggagaat aagggtttga atatctttaa ctccaaatat gtgctggccc
    10681 gcaccgacac cgccactgac aaagattacc tcgatattga acgcgttatc ggccatgaat
    10741 atttccataa ctggaccggt aaccgagtca cctgccgcga ctggttccag ctcagcctga
    10801 aagaaggttt aaccgtcttc cgcgatcagg aattcagctc tgaccttggt tcccgtgcag
    10861 ttaaccgcat caataatgtg cgcaccatgc gcggattgca gtttgcggaa gacgccagcc
    10921 cgatggcgca cccgatccgc ccggacatgg tcattgagat gaacaacttc tataccctga
    10981 ccgtttacga gaagggcgcg gaagtgatcc gtatgatcca caccctgctg ggcgaagaaa
    11041 acttccagaa agggatgcaa ctctatttcg agcgccatga tggcagcgcg gcaacctgcg
    11101 acgactttgt gcaggcgatg gaagatgcgt cgaatgtcga tctctcccat ttccgccgtt
    11161 ggtacagcca gtccggcacg ccgattgtga ccgtcaaaga cgactacaat ccggaaaccg
    11221 agcagtacac cctgaccatc agccagcgca cgccagccac gccggatcag gcagaaaaac
    11281 agccgctgca tattccgttt gccatcgaat tgtatgacaa cgaaggcaaa gtgatctcgt
    11341 tgcagaaagg cggtcatccg gtgaattccg tgctgaacgt cacccaggcg gaacagacct
    11401 ttgtttttga taatgtctac ttccagccgg tgcctgcgct gctgtgcgaa ttctctgcgc
    11461 cagtgaaact ggaatataag tggagcgatc agcaactgac cttcctgatg cgtcatgcgc
    11521 gtaatgattt ctcccgctgg gatgcggcac aaagcctgct ggcaacctac atcaagctga
    11581 acgttgcccg acatcagcaa gggcagccgc tgtctctgcc ggtacatgtg gctgacgctt
    11641 tccgcgcagt gctgctcgat gagaagattg atccggcgct ggcggcagaa atcctgacgc
    11701 tgccttctgt caatgaaatg gctgaactgt tcgatatcat cgacccgatt gctattgccg
    11761 aagtacgcga agcactcact cgtactctgg cgactgaact ggcggatgag ctgctggcta
    11821 tttacaacgc gaattaccag agcgagtacc gtgttgagca tgaagatatt gcgaaacgca
    11881 ctctgcgtaa tgcctgcctg cgcttccttg cttttggtga aacgcatctg gctgacgtgc
    11941 tggtgagcaa gcagttccac gaagcgaaca atatgaccga tgcgctggcg gcgctttctg
    12001 cggcggttgc cgcacagctg ccatgccgtg atgcgctgat gcaggaatac gacgataagt
    12061 ggcatcagga cggtctggtg atggataaat ggtttatcct gcaagccacc agcccggcgg
    12121 cgaatgtgct ggagacggtg cgcggtctgt tgcagcatcg ctcatttacc atgagcaacc
    12181 cgaaccgtat tcgttcgttg attggcgcgt ttgcgggcag caatccggca gcgttccatg
    12241 ccgaagatgg gagcggttac cagttcctgg tggaaatgct taccgacctc aacagccgta
    12301 acccgcaggt ggcttcacgt ctgattgaac cgctgattcg cctgaaacgt tatgatgcca
    12361 aacgtcagga gaaaatgcgc gcggcgctgg aacagttgaa agggctggaa aatctctctg
    12421 gcgatctgta cgagaagatc accaaagcac tggcttgata aataaccgaa tggcggcaat
    12481 agcgccgcca ttcggggaat ttacccctgt tttctcaggc gaatttcaga ttcaccacgc
    12541 tgcatcaccc tttgcaacac ttccgattcc agctccgcta atcgcaccga ccctaatcga
    12601 cgcggacggg ggatatccac tgtcaaatcc agaccaattt ttccctcttc aattaataac
    12661 acccggtcag ccattgctac cgcttcgctc acatcatgcg tcaccaacag tacggtaaag
    12721 ccgtgctcct gccaaagtga cacaatcaaa tcctgcatct cgagtcgcgt taaggcgtcc
    12781 agtgccccga gcggttcatc aagcaacaat aaacccggtc gatgaatcaa cgctcttgcc
    12841 agcgccactc gctgtttctg cccgcctgaa agtgcggcag gccattcccc ggcgcgattc
    12901 tccagtccta ccgcagccaa cgcccgacgc gcggcatcgc gccactggcc tttaaggcct
    12961 aacccaacgt tatcaatcac cgatttccac ggtagcagac gcgcatcctg aaacatcatt
    13021 cgcgtgtctt cctgaatttc agccagcggt gtggtgcctg ccaacacatc gcctgcggtt
    13081 ggcgtttcca gcccggccag caggcgcagt agggtacttt tgccaccacc gccacaaact
    13141 gacctgccgg aatatgtaaa tccagttggt tcaggacgat attttccgcg taatgtttgc
    13201 ttactgcatt gagcaacaat ggtgtgccct ggttcagacg agcagtattc ataccgtggc
    13261 ctccttcaaa tgataagccg ggttccagcg caaccagagg cgctctaaca gctgcgcgct
    13321 gacgtcagca agtttgccga gcagggcgta aagaataata gcgactacca ccacgtccgt
    13381 ttgcaaaaac tctcgcgcat tcatcgccag ataaccaatg ccggaattgg cagaaatggt
    13441 ttcggcaaca atcagcgtca gccacatcag gcccaacgca aaacgcacgc cgaccataat
    13501 tgagggcagg gcaccaggca ggatcacatg gataaacagc ggtatgccgg ataacccata
    13561 gctacgcgcc atctccacca gcccgcgatc gatattacgg atgccatgcc aggtgttgat
    13621 ataaatggga aacagcgtac cgagcgccac cagaaagatt tttgcggatt catcaatgcc
    13681 aaaccacaaa atcaccagcg ggatcagcgc cagatgcggc acgttgcgca acatctgaat
    13741 tgaggtatcc agcagccgtt ctccccagcg agatagcccg ctaatcagtc ccaaaatcaa
    13801 ccccagcgat ccgccaattg aaaagccaat cagcgcccgc taggaactga tcgccagatg
    13861 ctgccacagt tcgccgctgg cggagagcgt ccagaacgcc gtcaccaccc cttccggtga
    13921 gggcaaaata cgcgtcgaca gccagccaac tgaggaggcc agttgccaca ccgccacgat
    13981 gcccaccggt aaaaaccagg gggcaacgcg caataaccac ttcttcactg gcgttgccat
    14041 tatttcttcc ttagctttgc gcgactttac gggggataaa atcattcgcc accgcttcgc
    14101 cttgcggatt cagcggctgc ggctggggaa tttccggaat ggcgacatcc agatgtggga
    14161 acagcaactc gccgacccga tacgcttctt ccagatgcgg atagcccgaa agcacaaagc
    14221 tgtcgatgcc aagcgcggcg tattcgttga ttcgcgcagc gaccgtagga ccatcgccca
    14281 ccagcgccgt tccggcaccg ccgcgcacta agccaacgcc cgcccataaa ttggggctga
    14341 tctccagatt gtcgcgcttg ccgttatgta acgccgccat tcgctgttgt cctacggaat
    14401 ccgtccgggc gaatgcggcc tgtgctttgg cgatagtttc atcatcaaga tgcgagatta
    14461 accgctcggc ggcctgccac gcttcgtcgt tagtttcacg aacaatcaca tgcagacgaa
    14521 taccgaaacg aattttgcgt ccatgcgcgg cagctttcgc ccgcacttgt tcgatcttct
    14581 cttttactag ttctggcggt tcgccccagg tgaggtagag atcaacttgt tctgccgcca
    14641 gctcctgggc gacatctgac gatccgccaa agtaaagcgg aggataaggc tgttgaatcg
    14701 gcgggaagag cagttttgct ccgcgcacat gaatatgttt accgttgaaa ttgacggttt
    14761 cccccagcaa taagcgccgc cagacctagg taaattccgc cgaggcttcg tagcgctcgc
    14821 tatgatcaag gaacacgccg tcgcctgcca gctcttgtgg atcgctgcct gtgaccagat
    14881 taaacaacgc acgaccattt gagagacggt caagcgtggc ggcctggcga gcggcaacgg
    14941 taggtgaggt tacgctgggg cgcagggcga caagaaactt cagccgcggc gtcaccggga
    15001 tcatcgacgc cgccaccagc cacgcatctt cacacgatcg ccccgttggg atcagcacac
    15061 cggtatagcc cagacgatcc gccgcttgcg caatttgttg cagataaccg tgatcaaccg
    15121 ggcgtgaacc ttcttccgtt ccaagatagt gcccgtcacc gtgggtcggt aaaaaccaga
    15181 acatattcag actcataatt gttttccttc cagttgagtg ggctgccaga tgcgctggcg
    15241 aatatcgact tttttcggca ccagacgatt ttcataaaac agatctgccg tttgctgctg
    15301 taaggcggca acctcggcgt taaccggttt gatggtggta ggagggcgat gatctaagta
    15361 agaggcaatc accggtgccg gtaagcccat cgtttttgcc agtaaagcga tgctttgctc
    15421 gcgctggctg cgggttaacg catcggcctc actaaaggtt gccagtacgc cctgaataaa
    15481 agcgccgttt tttctgcata gggacgagct gccagataaa acgatccagt ttgattgaga
    15541 tcggtgccgt ctttcagcac ccgcacgccg ccctgtaata atgcagcaga gtagtaggga
    15601 tcccagatag cccaggcgtc aacgttacct tgctggaacg cggcgcgggc atcagcgggc
    15661 gtcaggtaag tgggttggat atcggtaaac ttaagtccgg cctgacgcag tgcacgcagt
    15721 aaaaggttgt gtgaactgga acctttctga aaggcaactt tgtgaccttt aagatcggct
    15781 acggttttga tcgggctgtt ttctgccacc agaatcactt cggctttggg cttcggtggc
    15841 tcgacgccca catataccaa atcagccccg gcagcctggg caaagattgg cggaatatcc
    15901 ccggtactgc cgagatcaat actgccaacg tttaaggctt ccaacatttg cggacccgcg
    15961 gggaactcca cccaggagat ttttgattgc ggatagcgtt tttccagtaa ctggtggctt
    16021 tttgccagta ccataccaat actgcctttc tgatagccta tacataacgc ttcaggcgag
    16081 gattctgcag caaccgcaat cgtagagacg ctaagcaatc ccaccagcgc cagtttaatg
    16141 atgttacgca tgggcattac ctcgcagaga cagaaggtca ggaacctgaa catcgcggcg
    16201 gtgcaatgcc tgccagaaag tttctagcgc ggtatcaaga cgggtttgca gatttggcgt
    16261 gaactggggt ctgtgatggt aatcaattac ttgtgagtca tcggcaaaca cgccgtgcag
    16321 gatctcctga gctttcagtg cgcttaaaac tggtttaagg gcataatcga ccgccagcag
    16381 atgggccacg gtaccgcccg tcgccagcgg tagcaccact ttgccttgca aagcgcgctc
    16441 tggcagcagg tcgagcaggg ttttcagcgc gccggaatag gcggctttat acacaggtgt
    16501 agcgactatc agtccatcag cctgttgcag ctgttcggtg aaggtcttga gtgccggact
    16561 atcgaaacga gcataaagca gatcttccgg ggcgaagttt tgcagattcc agt
//
LOCUS       DAJTNA010000082        16546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTNA010000082 DAJTNA010000000
VERSION     DAJTNA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16546
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.083"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            187..1464
                     /locus_tag="OO161_002257"
     CDS             187..1464
                     /locus_tag="OO161_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5463307.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPVMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATH"
     gene            1734..2528
                     /locus_tag="OO161_002258"
     CDS             1734..2528
                     /locus_tag="OO161_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5463308.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFEWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(2599..3048)
                     /gene="rplI"
                     /locus_tag="OO161_002259"
     CDS             complement(2599..3048)
                     /gene="rplI"
                     /locus_tag="OO161_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5463309.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(3090..3317)
                     /gene="rpsR"
                     /locus_tag="OO161_002260"
     CDS             complement(3090..3317)
                     /gene="rpsR"
                     /locus_tag="OO161_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5463310.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(3322..3636)
                     /gene="priB"
                     /locus_tag="OO161_002261"
     CDS             complement(3322..3636)
                     /gene="priB"
                     /locus_tag="OO161_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5463311.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(3643..4038)
                     /gene="rpsF"
                     /locus_tag="OO161_002262"
     CDS             complement(3643..4038)
                     /gene="rpsF"
                     /locus_tag="OO161_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5463312.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            4365..4640
                     /locus_tag="OO161_002263"
     CDS             4365..4640
                     /locus_tag="OO161_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5463313.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(4715..5266)
                     /locus_tag="OO161_002264"
     CDS             complement(4715..5266)
                     /locus_tag="OO161_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR5463314.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5363..6049)
                     /gene="ulaF"
                     /locus_tag="OO161_002265"
     CDS             complement(5363..6049)
                     /gene="ulaF"
                     /locus_tag="OO161_002265"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5463315.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYWQK"
     gene            complement(6049..6885)
                     /gene="ulaE"
                     /locus_tag="OO161_002266"
                     /pseudo
     CDS             complement(6049..6885)
                     /gene="ulaE"
                     /locus_tag="OO161_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
     gene            complement(6895..7545)
                     /gene="ulaD"
                     /locus_tag="OO161_002267"
     CDS             complement(6895..7545)
                     /gene="ulaD"
                     /locus_tag="OO161_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5463316.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(7559..8023)
                     /gene="ulaC"
                     /locus_tag="OO161_002268"
     CDS             complement(7559..8023)
                     /gene="ulaC"
                     /locus_tag="OO161_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5463317.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(8033..8338)
                     /gene="ulaB"
                     /locus_tag="OO161_002269"
     CDS             complement(8033..8338)
                     /gene="ulaB"
                     /locus_tag="OO161_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5463318.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(8354..9801)
                     /gene="ulaA"
                     /locus_tag="OO161_002270"
                     /pseudo
     CDS             complement(8354..9801)
                     /gene="ulaA"
                     /locus_tag="OO161_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            10106..11170
                     /gene="ulaG"
                     /locus_tag="OO161_002271"
     CDS             10106..11170
                     /gene="ulaG"
                     /locus_tag="OO161_002271"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5463319.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTIPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNLEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            11278..12033
                     /gene="ulaR"
                     /locus_tag="OO161_002272"
     CDS             11278..12033
                     /gene="ulaR"
                     /locus_tag="OO161_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5463320.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(12030..12779)
                     /gene="yjfP"
                     /locus_tag="OO161_002273"
     CDS             complement(12030..12779)
                     /gene="yjfP"
                     /locus_tag="OO161_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5463321.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDIVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            12961..13290
                     /gene="bsmA"
                     /locus_tag="OO161_002274"
     CDS             12961..13290
                     /gene="bsmA"
                     /locus_tag="OO161_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5463322.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQSTPQPALPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            13439..13714
                     /gene="yjfN"
                     /locus_tag="OO161_002275"
     CDS             13439..13714
                     /gene="yjfN"
                     /locus_tag="OO161_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5463323.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(13831..15456)
                     /locus_tag="OO161_002276"
     CDS             complement(13831..15456)
                     /locus_tag="OO161_002276"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5463324.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(15540..>16546)
                     /locus_tag="OO161_002277"
     CDS             complement(15540..>16546)
                     /locus_tag="OO161_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5463325.1"
                     /translation="NGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLE
                     VVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAI
                     QERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGV
                     GGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLL
                     WRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDY
                     ADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG
                     "
BASE COUNT         4234 a         4346 c         3970 g         3996 t
ORIGIN      
        1 tgacaaggtc atcgggcatt atctgaacat aaaacactat cagtaagttg gagtcattac
       61 cgacttttta aagggagaaa tttacacaca ataattttca atatttcctc tgtacctgat
      121 ttttattacc tattacacgg aaataccaac aaccagggtt tattaatata attaaaaggt
      181 ttaaacatga ataaaataaa gaattaccgg tggcatatga ttgccctcgt atgctttatc
      241 actgtaatca attatctgga cagaacggcg ttaggtattg cggctccaac gattatggag
      301 acaaccggaa taactaaaga gcaatattca tggattgtca gtgcattcca gttggcctat
      361 acattagggc aaccggtaat gggcttcttt attgataccg tgggtctgaa gttaagtttt
      421 gcgatatgtg ccgcaatttg gggcctggcg acaatgggcc atgcactaac cggaacgtgg
      481 tctggtctgg catttatgcg cgccctgatg ggtttcagcg aagcgtcggc aattccggcg
      541 ggtgtaaaaa ccgcatcaac atggttcccg gcaaaagagc gtggcgtggc gacaggtgtt
      601 ttcaatatgg gcacctcact cggcgcgatg cttgctccac cgttgattgc ctggtgcatt
      661 atgtttcata gctggcaatt tgcgtttatt gtttcaggta gtcttgcttt gctcgcggct
      721 ttattttggt tcttttgtta taaagatccg aaagatgcca aacgcctttc tgatgaagag
      781 cgccactata ttgaatcagg gcaagaacag catcttaaaa cagataagaa agaaaaaacg
      841 tcaatcaagc atatcctcag ccaacgtaat ttctggggga ttggcatcgc gcgttttctc
      901 gcagacccgg catggggaac cattaacttc tgggtgccga ttttcttcgt cgaaacgctg
      961 cattttagcc tgaaagaaat tgccatgttc gtctggctgc ctttcctgct gggcgatctc
     1021 ggctgtttag ccagtggttt tgtcgcgaag ttcttccacg atcgcggcgt gagtttaatt
     1081 aactcacgaa gaattacctt cactattgca gccgtcatta tgatgacgat tggcctggtg
     1141 agtattgtcg aaaatcccta cattgccgta ttactgatta gtattggcgc gttctcgcat
     1201 caatgtcttt ctactgtagc agcaactctg ggtggcgacc tgttcaaaaa agacgaagta
     1261 gctaccgcag tgggtatggc aggagcctgt gcgtggagcg gtcagttgat tttcaacctg
     1321 ttcatcgggg cattcgttca cattatcggc ttcgcgccgt tctttattgc cctggctttc
     1381 tttgacatta ttggtgctat tgcgttgtgg acgcttatca aagttaaaga tgaagaacca
     1441 caagtacagt tagctaccca ctaattattt taaataaaaa cgctataatt gaggttatgg
     1501 cgtttttaaa gaagcttgag ttatcgaacc tcgcttacca acgcgttgtc agtcatctcc
     1561 cccctcccta gtgcatcgtt atatccgtga tgagcgattt ttgatattaa tatctttcac
     1621 catccgctat tttcaccact tcagaaaaca aacttatttt tattaatatc aattcttttt
     1681 ctttgaacat tcaactgcga tatttgtaaa aacattaaac aggcaacaat aaaatgacat
     1741 taccaacaac tatttattca ttccccgcat atttacctcg tttttcgtcc accgacaaac
     1801 cagcaaaatt aaaatttcat caatacgcac gcgctacatt actgtcaaat cgtggaagag
     1861 atcataattg cgatggcaga cgtactgttg agatccacaa actagatctc tctgactggc
     1921 aagcattcaa taagttagca acgaagtgta atgcttacga tggagtaaca atgaatggtg
     1981 ataattcttt tgaatggaac catgaagcta cattagataa tattcacgcc cagaaataca
     2041 ataaagctta cgcgggtgcg aggttaaccg cagaactgaa atatttattg caagatgtcg
     2101 aatccttcga acccaacagt aaatacacaa tacacgaagt ggttttggga cctggttatg
     2161 gtactccaga ctataccggc caaacgatag gatacgttgt aacccttcct gcccaaatgc
     2221 ctaattgctg gtcatctgaa ctaccaacaa tcgatcttta tatcgaccaa cttcgcactg
     2281 ttacaggcgt ttctaatgcc ctcggattca tcattgctgc attgctaaac gcgtattctg
     2341 atttacccca tgatttgaaa attggtctgc gttccttatc aaattcagca gcaatctact
     2401 cgggtttagg tttcgagcga gtgccgcagg accgagacat aaggagtgat cgtatgcatc
     2461 taacgccagc aaaccatcct gacttatgga cacaagagaa tggtgaatgg atctacttgc
     2521 gaaactaata cctgacgaaa aatagaaagc aaaacgccga ccaatggtcg gcgtttttac
     2581 gtctcgttga ataacgaatt attcagctac tacgtttacg atcactttcg cgaatacttc
     2641 gctgtgaacc tggaagctca cttcgtgttc gccagtggta cgcagaacgc cgttcggcag
     2701 acgaacttcg ctcttagcca cttcaacgcc agctgcagtt acagcgtcag cgatgtcgcg
     2761 agtaccgatg gaaccgaaca gtttaccttc gtcgccagct ttagacgcga tggtaacagt
     2821 ttccagtgca ttgattttct cagcgcgagc attagcagct gccagaactt cagccagttt
     2881 agcttccagt tcagcgcgac gtgcttcgaa gaattcaatg tttttcttgg tagctggaac
     2941 agctttaccc tgcggtacca ggaagttacg agcatagccc gctttaacgt ttacctgatc
     3001 acctaggcta cccaggtttg ctactttatc aagcagaata acttgcatta ccttatcctc
     3061 tcaaagtcgt attaatggac cgtgaccgat tactgatggc gatcagtgta cggcagcagg
     3121 gacaggtagc gagcgcgttt gatagcgcga gccagctgac gctggtattt tgcacgggta
     3181 ccggtgatac ggcttgggac aatcttaccg ctttcggtga tgtagttttt cagcgtagcg
     3241 atatctttat agtcgatctc ttgaacgcct tccgcggtga aacggcagaa cttgcgacga
     3301 cggaaataac gtgccatatg gctagtctcc agaatctatc aattcaatct gctcggcatg
     3361 caaaaccatt ttgctcagtc cgttctttgc cttgtggcat gaaatgaacc cctgaacggt
     3421 tatgcgactg ccgaccgtta tactgtgagt aatggcctgg ttttcgtgtc cgctaacaat
     3481 aacgggcatt tggcaccacg cctgccggtg aaaaccggct tcctcctgca cagaacgatg
     3541 ctcaagcacg aactggcagt gaggaattcc tgatggactg acctttcgaa ggggagtcct
     3601 gcacacggtg ccggacaaca ccagacggtt ggtcatcaga aattactctt cagaatcccc
     3661 agcttcagca tcatcagcgg tttcgtttgc gaaatcatcg cgacgctcac ggcgctcgtc
     3721 tttcgcttta accatcggag atgcttcggt aacagcgtgc ttggtacgca taaccatgct
     3781 gcggataacg gcatcgttga agcggaaggt agtttccagc tcatcgatca cttcctgcgg
     3841 agcttcaacg ttcatcagaa cgtagtgtgc tttgtgcagt ttgttgatcg ggtaagccag
     3901 ctgacggcgg ccccagtctt ccagacggtg gatcttgcct tctgcaccag tgatggcagc
     3961 agtgtagcgc tcgatcatgc ccggaacctg ttcgctctgg tcaggatgga ccataaaaac
     4021 gatttcgtaa tgacgcatcg aattgctcct tacggattat tcagcctcct gtctgggtca
     4081 gccgaatccc ggggaggcaa ggaacgtgtt aaaggtcggc tgaaaaatga cgcgttatag
     4141 tacttgtatg ccccactaaa ctcaaggcca gccgacaaat aaatctcatc tttcgctcaa
     4201 aagaactaac tcgctgattt gaagttcatc acattccgtc acagtgtttt ttcgaacaac
     4261 tgcatcagaa atggtcacca tcaacttctt tatcattgat taaaattaaa tcagaagatc
     4321 atcacagagg agagcacaca aagtcagccg tcgaggaggt atctatgttc agtcgcgttt
     4381 tagcccttct ggctgtgctt ttgctaagtg caaatacatg ggcagccatt gaaattaata
     4441 accaccaggc cagaaatatg gacgacgtac agagcttagg cgtgatttat atcaatcata
     4501 atttcgccac tgaaagcgaa gcacgtcagg cattaaatga agagacagat gcgcagggcg
     4561 caacgtacta ccacgtaatt ctgatgcggg aaccggggag taacggcaat atgcacgcca
     4621 gcgcggatat ttatcgctag caccaggtat aaccaacgaa acattgccat agtttgcttt
     4681 gcccccttcg cagggggctt ttttatacgg ttattcaccg gattgcgact tccggatctg
     4741 gctcatgagt aatgcgatta cgtaagtcgc ggcgcataat ttctattatc cagaaccaga
     4801 aaatatgccc caggatttca gaggcatatt cgtcaaaagg caattgagtg agcggcggcg
     4861 ttaaattcag cgccgggcaa aatataccgt ggactgccac tgtcacaata agccctgcta
     4921 atatcccctg ccataatttc actttcggga aaatttccgc gacaatacaa taccctatcg
     4981 caaaaaccag tgagaagatg atatgcgtca ccatcaccgg gttgataata tgttccgaga
     5041 atgtatacac agtttgggtt gggtcgatac ctaaataatc ccgtagaaag atatacggcg
     5101 ggttaaattc atcgcgacca ccggaaaatg tgcgcggtgg caaggggact tccgctcccc
     5161 atttaacaaa tgctgaaata attcctgaaa gtacacccac aagtaacgca acgccatagc
     5221 ggcgacgttg cggatccgtt ctgacaaaca atccattgat agccataaaa acacctgttg
     5281 tttatacgat tttttaactg aagcggcccc ggaatcagac caattacatc ccgcaggagg
     5341 taataattcc ggggcgcact gcttacttct gccagtaata ggcgttagga ccgtgcttac
     5401 gcatgaagtg tttattcatg aggaagctgt cgatgtgatt cagttgtggg ttaatactgc
     5461 gcgcaatcca cgccattttc gccacttctt ccatcaccac cgcgttatgc accgcgtcgt
     5521 gggcatcttt cccccaggcg aacggcccgt gctgatacac cacaattccc ggcgtatgca
     5581 gcggctcggc gtcacccagc gtttcgataa tcactttgcc ggtgttcagt tcatactcgc
     5641 cctgcacctc ttcttcgctt aatccacgcg tacacgggat gtcgccaaag aagtagtcgg
     5701 cgtgcgtggt gcctaacgcc gggatcgcca gccccgcctg cgcccatgcg gtggcatgag
     5761 ttgaatgggt atggacaatg ccgccgagcg acgggtaacg acggtagagt tcaagatgcg
     5821 tcgcggtgtc agaagacggg cgatacttcc cttctaccac cttgccgctc atatcgacca
     5881 ccaccatatc gtctgctttc atggtttcgt aggcaacgcc gctgggcttg atcaccacca
     5941 gcccgcgttc gcggtcgata gcgctgacgt tgccccaggt aaaggtcacc agcccatagc
     6001 gcggcagatc catgttggct tcaaacacct gctgttttag cttttgcatt atgccgcctc
     6061 caccatgcct gctttggcca tgcgcgcttt caccacttct gccgccgggt cttccgccgt
     6121 ttcgctccac atctcaatca ggtacggccc gcaatagcca ctctgtttga gcgtttcgaa
     6181 gcaacgttcg aaatccacca caccttcgcc aaatggcacg tttttgaaga cgcccggttt
     6241 ggtgtctttc acatgcaccg cgacgatatg cccgattccg gcctgcaact ccatctgcac
     6301 atcgttgtcc cacgccgaca ggttgccgat atctgggtag agctggaacc acgggttgtt
     6361 gagatagtgc gcgtaaccca gcgccttgct gatggagttc atcaacggat aatccatgat
     6421 ctccatcgcc agcgtcacct gcgcgcggct cgccatctca acgctctctt tcaggccgtc
     6481 acggaaacga cgacgcgttt cgttattggc ttcctgatag taaacgtcat agcccgccag
     6541 ctggatcacg cgaatgccaa cgtcctaggc gaactggatg gctttacgca taatctccag
     6601 cccctgcgcc cgcaccgcgt catcttcgct gcccaacggg aaacgacgat gggcagaaag
     6661 gcacatggac ggcacacgca cgccggtttc aacaatcgca ttcaccagcg ccagacgctg
     6721 ctcgcggctc cagtcgaggc gcgacaggcg ctcgtcagtt tcatctaccg acatctcgac
     6781 aaaatcgaag cccaacgttt ttgccagtcg caggcgttcc agccagcact ccccggcggg
     6841 gagcgctttt tcatagatgc caagcgggat ttgtttggac aacatatccg ctccttagcc
     6901 ccacagttcg gcgatggaac gtttgaactg acgcgcggct tcaaccggag aagcggcatc
     6961 acggatgctg cgaccggcga taaacacatg aattggaatg cctttgaaca gcggcagatc
     7021 ttccagcgcc aggccaccgg tgacggtgac tttgaagccc atatcggaaa gacgtttgat
     7081 cgcagtgata tcggcttcac cccacgccac gccagcggcc tgcgcgtcac ggctgcggtg
     7141 ataaaccacc tgctgaatac ctgcgtcgcg ccactgttgc gcctgttccc aggtccagta
     7201 accggtcagt tcgatctgca cgtcgccgtt aaactctttt gctacgtcca gcgcaccttt
     7261 ggcggtgttg atatccgcac aacaaatcac ggtcacccag tcagcgttgg cttcgaagca
     7321 catacgagaa agaattttgc ccgcatcggc aattttggcg tctgccagca cgattttgtg
     7381 cgggtagagc gctttcagat cgcgaaccgc acgcacgcct tcgcccacgc acagaatggt
     7441 acccacttcg ataatgtcga cttcttcggc aatcaggcga gtggtttcgt aggcgctatc
     7501 catagtctgg ttgtccagcg cgacttgcaa catcggtaat gacatcttca attccttctt
     7561 aagctgccgc gttggtgcgg tcaatgagat ccagtacttc ctgctcggta cggcaggcac
     7621 gtaaacggtc gaaattttct tcatcttcaa acaggttgac gatctgcatg atgcccactt
     7681 cctggtgcgt gttggcatcg accgccgcca tggtgatgag gatatccacc gggtcgttat
     7741 cttcgtggtt gaactccagc ggctttttca gcgtcaccag cgcaaaaccg gttttcttaa
     7801 cgccctcttc cgggcgaccg tgcggcattg ccaggcctgg tgcaagcaca aagtacggac
     7861 cgtgctgttc aaccgcatcc agaatcgcct ggtagtaacg cggctcgaca acatccgccg
     7921 ccaccagcag gtcaacgccg atttttactg cgtcctgcca tgtctcagct tcagcctgca
     7981 ggcggatgga tttattctcc gccagcgaat cacgtaattt catggcgcgt ccttacttca
     8041 catcctgcgg gaaatgcgct ttgatcactt ccagcagttt cgggccaaag tcggcaggag
     8101 agagcatgtt gcgcacgcca accacgtatt tgttgccggt cacggtgatt tcgcccgcaa
     8161 tgtgcgtaga agcgatgatg atatccgcgc cgctcaactc gcttttgtat tcgccaaccg
     8221 cgcagctatt taccgtgtgg tcaatgtttg attgggttaa aaactggtcc actttcatct
     8281 tcatgatcat ggaactgcct tgtccgttgc cacacacagc cagaatacgt acggtcataa
     8341 tcaaaactcc ttattaagca gactgttctg ccagttgttt ttctgcatct tcttctgcgc
     8401 gcagtgcgcg gccagcgaag aacatataag cagtgcaatt acaatgatga cggccataaa
     8461 ggcgataccg atggagaaga agccctgcat catcggcggt gccagaatcg accagtccgc
     8521 catgcccatc caggcgctca taccggtgag tttcaccgcc cagacgcagc caaagatttc
     8581 aatcatcccc atcaccagac atatcttcag cgccgcacgc cagccgccga agtggttagc
     8641 gaacacgccg atggtggcgt tggagaagaa catcgggata aagccgggaa taatcaggat
     8701 cgaggaaccg caggcgacca ggatgccaac cgcaatcagc tgaccgatgg tgccccacat
     8761 aaagccccaa accacggcgt tcggcgcgaa gctatagata gccgcacagt caatcgccag
     8821 aaccgcacca gggatcaggc gctgggagat accgttaaat gcttcagaga gttccgccac
     8881 aaacatgcgc acgccctgag tgatgatgaa gatcgccacc gcgaaggaga aaccagtttg
     8941 caggatgtac accgtccagt tcactttgcc tgccatcgcc tgcacggtgt cgataccgaa
     9001 ggagagcaga atggcaccaa agaagatggt catcacaatc gccgtggaga cgatgttgtc
     9061 gtggaaaata ttcagccagc ctggcagttt gaggtcttca acgctttcct cttttttgcc
     9121 gaggaacggc gcaactttat aggcaatcca tgatgcaaac tgttgctggt gaccgatgga
     9181 gaaaccgcag ccgtccgtca cttcctgagt cggcttgtac atcatgttgg aggtaatgcc
     9241 ccagtagagc gaaaccagaa tcgccgtgca gataatggtt gtccacatgg agtagccgaa
     9301 aatgaacaac gtaacggcaa tcagccccgc ctgctggaac atgatatgac cagtcagcat
     9361 gatggtacga ataccggtaa tgcgacgcag cagcacgtaa cagatgttca gcgccagcgc
     9421 taacaacacc gcgtaaccta cccagctata ggcatcgccc atgcggtcga tggttgccat
     9481 catcgacgcg taagtatcgg aaattgcgcc gttaatgccg tagacttcgg acattttcgc
     9541 caccaccggt ttgaaggtgc tggtgaggat gccggaccct gcctgcaaca acatgaaacc
     9601 aattatggtt ttaatcgtgc ctttaataat aacgctgaca cttttgcgca gtaggatgta
     9661 gcccagacag gtcacaatac ccagcaacaa cggggcattg gtcatgacct ggttaaaaaa
     9721 cacggtaaag atgttgtaga gacgatctcc tgaagaagag gtaaccgggt attccgcaca
     9781 cccggaatgt tgtgcactca ctctaatttt caaaagtaat cacaacaaga ttattttatg
     9841 attaaatgtg acgcgacccg caaataattc catacggata gagtgggatt cacacaacga
     9901 aacaattatt tatttaaatt tatttaaatt catttagtta atttaactac acattccatt
     9961 aaatctttcc acactcaaaa tcaacaacaa cttccctggc aaaaagtcaa aaaatggtca
    10021 ttgccttcct gaaaaaatag tgtcaggatt aatcaaaacc aatcacatat tgattcgaat
    10081 tgaactatca tgaaggtaaa tggcgatgag taaagtgaaa agtatcaccc gagaatcctg
    10141 gatcctgagc actttcccgg agtggggtag ctggctgaat gaagaaattg aacaagagca
    10201 ggtcgcccct ggcacatttg cgatgtggtg gctaggctgc accgggatct ggttgaaatc
    10261 agaaggtggc accaacgttt gcgttgattt ctggtgcggc actggcaaac aaagtcacgg
    10321 taacccgtta atgaaacagg gtcaccagat gcagcgcatg gctggcgtga aaaaactgca
    10381 gccaaaccta cgcaccatcc cgtttgttct tgatccgttt gcgattcgcc agatcgacgc
    10441 ggtactggcg actcacgatc acaacgatca tatcgacgtt aacgtcgctg ctgccgtgat
    10501 gcagaactgt gctgatgacg taccattcat tggaccgaaa acctgcgttg atttgtggat
    10561 tggctggggc gtaccgaaag agcgttgcat cgtggtcaaa ccgggcgatg tggtgaaagt
    10621 gaaagacatt gaaattcatg cgcttgatgc tttcgaccgt actgcactga tcaccctgcc
    10681 tgccgatcaa aaagcggctg gcgtactgcc ggacggcatg gacgatcgcg cggtgaacta
    10741 tctgttcaaa acgcctggcg gctccctgta tcacagcggc gactcccact actctaacta
    10801 ttatgcgaag cacggtaacg aacatcagat cgacgtggct ttaggttcat acggcgaaaa
    10861 cccgcgcggt atcaccgaca aaatgaccag cgccgatatg ctgcgtatgg gtgaagccct
    10921 gaatgcgaaa gtggttatcc cgttccacca cgatatctgg tcaaacttcc aggccgctcc
    10981 gcaagagatc cgcgtgctgt gggagatgaa aaaagatcgt ctgaagtatg gcttcaagcc
    11041 gtttatctgg caggttggcg gcaaatttac ctggccactg gataaagaca acctcgagta
    11101 ccactatccg cgcggtttcg atgattgctt cactattgaa ccggatctgc cgttcaagtc
    11161 attcctgtaa tctgtcgttt ataccggata gcgtggagaa gttcgcgcta tccggcgtag
    11221 tatttcctgg ctatttcaaa tatcatctaa aaaaatcaaa ttttatcgga atagctcatg
    11281 actgaagcac aaagacatca aatcctcctg gaaatgctcg cacaattggg ctttgtgacc
    11341 gttgagaaag tcgttgagcg tctgggaatt tcgcctgcca ctgcgcgacg cgatatcaat
    11401 aaacttgacg aaagcggcaa actcaaaaag gtgcgcaatg gcgcagaagc tattacccaa
    11461 cagcgcccgc gctggacgcc gatgaatctg catcaggcgc agaatcacga tgaaaaagta
    11521 cgtattgcta aagcggcctc gcagctggtt aatccgggcg aaagcgtagt catcaactgc
    11581 ggctccaccg cgtttctgct tgggcgggaa atgtgtggta agccagtgca aatcatcact
    11641 aattatctac cgctggcaaa ttacctgatc gatcaagaac atgacagcgt gatcattatg
    11701 ggtggacagt acaacaaaag tcagtccatc actttaagcc cgcagggcag cgaaaacagt
    11761 ctctatgccg ggcactggat gtttaccagc ggaaaagggc tgaccgcaga agggttgtat
    11821 aaaaccgata tgctgacagc aatggcagag cagaagatgc tgagcgtggt agggaaactg
    11881 gtggtactgg ttgatagcag taagattggc gaacgcgcgg gaatgctttt tagccgtgcc
    11941 gatcaaatcg atatgcttat caccggcaaa aatgctaacc cggaaatcct gcaacaactg
    12001 gaagcgcaag gggtcagcat tctgcgtgtt taaagatgct ggcggaaaaa tgtcacggca
    12061 gcatctaacg cctcaggcgt aatgcggtga cgcacgcctg gctgccatga acaggttagc
    12121 agtttatccc gtcccgtctc gcttaaggcc tgctgcaaac gtagtgattc gtcggcaggc
    12181 acaatgtcat cgtccaggcc atgccacagc agtagaggtc tgtccccaag ttgttccagg
    12241 tggtttgtcg cttcccactc tgccagcggc gcgataatgt tattgaattc attctgctgt
    12301 gctgccgttt caggtatcag cggtggaaac agtgaacggg cgagcgatgt aaaatagccc
    12361 gatcccatca tgctggcggt acatttcacc gtggggtggc gagcggtaat tcccagtgcc
    12421 gtcatcgcgc ccatcgacgc gccaccgact gccagacggt catcaagcag ccagttttct
    12481 tcggctattg ccgcacgtaa agtagtgaat tcctgcatac tttgtagcaa gatttgccag
    12541 aattgattta accgccgcgc ttcgtcgcta ctaaaacggc taccgtgatc gggcgcatcc
    12601 ggcatgatca cccgcaaacc agcctgcgcc agcgcaacgg caaaatagct atacaccagg
    12661 ctggatgaag taaagccgtg ataaaaaatt acgcacggta acggggtatc tttttgcccg
    12721 acaggataag catgaagaac gggaatatct gccagctcgc gtgattctat ttcaatcatc
    12781 tgtctctcct ttttgtctgg tatgacatga agaaatcctt agcacaagtt tctgctggcg
    12841 gtattagttc ataatgttga gatgtgggtt acgctttcgt cacgttttca ttcgaaaatc
    12901 gcgtactagg taacaattcg ggaacattcc cccaaagtta aattaataag gcactacact
    12961 atagttagca ggaaacgtaa tagcgttatt taccggtttg ccagtttatt attggtgttg
    13021 atgttaagtg cctgtagcgc actgcaaagt acgccacagc cagcactgcc agtaacgaac
    13081 catccgcaag agattcgccg cgaccagacg caagggttac agcgaatagg tagcgtaagc
    13141 acgatggttc ggggttctcc ggatgacgca ttagcagaaa tcagagcaaa agctgtcgct
    13201 gcaaaagctg attattacgt tgtcgtcatg gtggacgaaa ccatcgtgac aggacagtgg
    13261 tattcacaag ccattttgta ccgtaaataa atcagacaac ttacatagtc agctttacat
    13321 tgctttgcgt ccctatgcgt ttacctgtgc acaataaatt accggctgga agcctgggcg
    13381 tttattaccg cgatggaatg ccctgcaaca tgtggggaaa cgaaaaatgg agctgacgat
    13441 gaaacaatta cttgcttcac cctcgctgca attagtgact tatcctgcga gcgccacggc
    13501 acagtctgca gaattcgcca gtgctgactg cgtgacgggc ctgaatgaaa taggtcagat
    13561 ctcagttagc aatatttcag gggatccgca ggacgtggaa cgtatcgtgg cgttaaaggc
    13621 cgatgaacaa ggtgcgtcat ggtaccgcat tattaccatg tacgaagacc agcagcctga
    13681 taactggcgc gtacaggcga tactgtatgc atgagtgtca tctacagcaa atgaacaatg
    13741 ccggattcgg cgcgagcgcc ttgtccggcc tacaattttc acatatttca ttgagttaat
    13801 cgaaaccagc gtcgcatcag tcgtatacgc ttacacacac actccccccg tcgcccgcag
    13861 caataaatca ttctggatct gctctgacaa ccgtacgccg ccgcgcgtat ctaacatcac
    13921 ctgacaccac gcctgcgcca ttggcggaga agcatatttc aacatttgcg caccgcagcc
    13981 cagcaggaat agctgatgag taatctctcg ccccagttct tcagctggct tacgcagctg
    14041 ctgctgtaaa cgacgaaccg cgcgatcaaa atagcgatcc tgccctttca cttccacaaa
    14101 tgcttccgac aataagtcgt atacgcccgc ttgcttattg agaacgcgca acacatccag
    14161 gcacataata ttgccggaac cttcccaaat actgtttacc ggcatctccc ggtaaagccg
    14221 cggcagctcg ctctcctcgc aataaccaat gccgcccagc acctccatcg cttcggccac
    14281 aaacggcata cctcttttgc agatcacaaa ttttgccgca ggcgtaaata aacgcgccca
    14341 cagggcttct ttggcatcgg cacgccggtc ccacgctcgc gcaagacgaa acagcaacgc
    14401 cgtttgccct tcaagctgaa gtgccatgcg acttaagaca tgacacataa ggggctgttg
    14461 gatcaatgga ttaccaaaaa catggcgttg atgtgcatga taaatcgcca gcgaaaatgc
    14521 acggcgcatc atggcatggc tacccagggc gcaatcaaaa cgcgtcatcc cacccatttt
    14581 caggatcaga cgaattcctt ccccttcctg ccccaacaac cagccaatgg catcctgaaa
    14641 ctccacttcg caactggcgt tagagcgatt acccagctta tctttcagcc gctcgaggcg
    14701 aatcgcgttg cgttgcccat caggcaaaaa gcgcggcaca aaaaagcagg acaaaccgcc
    14761 cgtagtctgc gccagcacca gatgcgcatc gctttgcggc accgagaaaa accatttatg
    14821 ccccaccagc cgataagagc catcttccag acgctctgca cgggtggtgt tgctcatgac
    14881 atcggaaccg ccctgctttt ccgtcattcc catgccaatc aacaaaccgc gtttttgccc
    14941 acctggcaat aagtgagaat cgtagcgatc gctcagcagc ggcgtggtcc agtcctgaaa
    15001 cggcgtgggt aacatctgta acaacaatgg cgtggcggca aaggtcatcg ttattggaca
    15061 taacgaccca gcctcaacct gcgcatgtaa cataaaacgc gccgcgcgcg ccacaaatgc
    15121 gccggagcga gcgtcttctt cccaggcaag attgtgcacc cgattggtac ataacgcctg
    15181 catcagcagg tgccaggcgg ggtgaaaacg cacatcgtcc agacggcgtc cttgcgcatc
    15241 gtagcgcaat aattcaggcg gattcacatt cgccagccgc cccagttcaa gagattcagc
    15301 cgttcctaac tgctgaccaa tactggctag aaaatcgcta tcccagccag caccttcacg
    15361 cgttaccgct tcacagagcg cgccatcaga caggtataag ttgctgttat ttaatggtat
    15421 aggttgatta aaaacggtgt gagtttgcca gtgcactgtg tctccctcca tcaatggcag
    15481 tcacccttag tatggtcact gccatggatt cctgacagaa agagattgct aaaacattct
    15541 tatccagcaa tgtaatgcgg aacgaaacgt gaggtgtctt tggtgatcag cgtgttatct
    15601 tcacggatcc ccattccgca cgcttcatca tcgacaatcc agctaccgat gagtgtgtag
    15661 ctatcgccaa atcgcggcag aggttgaaac gcctggtaga tcatcggttc atcggcgtaa
    15721 tcaccatcag cgtggtcaac gacgttattc tgaccgtcga aaatggtgac gtttccgcct
    15781 tcgcgtgagt agatcggttt acgcacatag ctttcgccag cggcaatctg cggtttttcg
    15841 ccctcaaacc aggatgcaag aaggttagga tgacctggaa agaagcgcca aagcagcggc
    15901 attagccctt tattactcaa gatacttttc cataacggct ccacccattg ctcgcgacgc
    15961 ttgcgcagca gcggaccgtt atcgtcacgc atcatccact ccagcggata cagcttaaat
    16021 gcacgctgga tgacattatc atcaagatcg gtcagtacgc cgccgacgcc caaaccgaga
    16081 tcttcaatgt agataaaccg cgactcctgc cctgcctgct gggcgcagtc ctgcaagtac
    16141 agcacggtac tcctgtcttc atcggtgtcc tgacagcagc aaaaataaaa cggttcccga
    16201 ctgtaaagct cactaaagcg cgaaatcagg cgttcctgaa tggcgttgta ctccagcagc
    16261 ttcaccggcg cattgccaca ccaggcaaaa tccatccggc catacagcga aggatcgcgt
    16321 gcgcgccagc tttcggcgat cacatcccag tacaacggcg ggatcgccag ttgcgtcagg
    16381 atctcttcgt ctttaaccgc gcgatccacc acctcaaggc acatctgatg cagttccgca
    16441 gtcggttttt cgatctgctc ttcaatctgg cgcagagtaa aacggtaagc ccgactctcg
    16501 tcccaataga tttcattgtc gatgatatga aagtcgaaac cgttgt
//
LOCUS       DAJTNA010000083        16499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTNA010000083 DAJTNA010000000
VERSION     DAJTNA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16499
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.084"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..749)
                     /gene="mak"
                     /locus_tag="OO161_002278"
     CDS             complement(<1..749)
                     /gene="mak"
                     /locus_tag="OO161_002278"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5463326.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVL"
     gene            874..1785
                     /gene="rdgC"
                     /locus_tag="OO161_002279"
     CDS             874..1785
                     /gene="rdgC"
                     /locus_tag="OO161_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5463327.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(1863..1946)
                     /locus_tag="OO161_002280"
     CDS             complement(1863..1946)
                     /locus_tag="OO161_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463328.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(1943..2284)
                     /locus_tag="OO161_002281"
                     /pseudo
     CDS             complement(1943..2284)
                     /locus_tag="OO161_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(2432..2716)
                     /gene="ppnP"
                     /locus_tag="OO161_002282"
     CDS             complement(2432..2716)
                     /gene="ppnP"
                     /locus_tag="OO161_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5463329.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(2788..3465)
                     /locus_tag="OO161_002283"
     CDS             complement(2788..3465)
                     /locus_tag="OO161_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5463330.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(3723..3914)
                     /gene="yaiA"
                     /locus_tag="OO161_002284"
     CDS             complement(3723..3914)
                     /gene="yaiA"
                     /locus_tag="OO161_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5463331.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(3964..4488)
                     /gene="aroL"
                     /locus_tag="OO161_002285"
     CDS             complement(3964..4488)
                     /gene="aroL"
                     /locus_tag="OO161_002285"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5463332.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTING"
     gene            complement(4671..5129)
                     /locus_tag="OO161_002286"
     CDS             complement(4671..5129)
                     /locus_tag="OO161_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5463333.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5249..6058
                     /gene="proC"
                     /locus_tag="OO161_002287"
     CDS             5249..6058
                     /gene="proC"
                     /locus_tag="OO161_002287"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5463334.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(6075..7190)
                     /gene="adrA"
                     /locus_tag="OO161_002288"
                     /gene_synonym="dgcC"
     CDS             complement(6075..7190)
                     /gene="adrA"
                     /locus_tag="OO161_002288"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5463335.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(7292..7612)
                     /gene="psiF"
                     /locus_tag="OO161_002289"
     CDS             complement(7292..7612)
                     /gene="psiF"
                     /locus_tag="OO161_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5463336.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSGCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(7731..9146)
                     /gene="phoA"
                     /locus_tag="OO161_002290"
     CDS             complement(7731..9146)
                     /gene="phoA"
                     /locus_tag="OO161_002290"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5463337.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQSVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(9247..9507)
                     /gene="iraP"
                     /locus_tag="OO161_002291"
     CDS             complement(9247..9507)
                     /gene="iraP"
                     /locus_tag="OO161_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5463338.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            complement(9734..9891)
                     /locus_tag="OO161_002292"
                     /pseudo
     CDS             complement(9734..9891)
                     /locus_tag="OO161_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9969..11063
                     /gene="ddlA"
                     /locus_tag="OO161_002293"
     CDS             9969..11063
                     /gene="ddlA"
                     /locus_tag="OO161_002293"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5463339.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(11087..11299)
                     /locus_tag="OO161_002294"
     CDS             complement(11087..11299)
                     /locus_tag="OO161_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5463340.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            11559..11867
                     /locus_tag="OO161_002295"
     CDS             11559..11867
                     /locus_tag="OO161_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5463341.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(11926..13020)
                     /gene="yaiW"
                     /locus_tag="OO161_002296"
     CDS             complement(11926..13020)
                     /gene="yaiW"
                     /locus_tag="OO161_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5463342.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPDLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(13033..14253)
                     /gene="sbmA"
                     /locus_tag="OO161_002297"
     CDS             complement(13033..14253)
                     /gene="sbmA"
                     /locus_tag="OO161_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5463343.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            14605..15762
                     /gene="ampH"
                     /locus_tag="OO161_002298"
     CDS             14605..15762
                     /gene="ampH"
                     /locus_tag="OO161_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5463344.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(15763..16386)
                     /gene="iprA"
                     /locus_tag="OO161_002299"
     CDS             complement(15763..16386)
                     /gene="iprA"
                     /locus_tag="OO161_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR5463345.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         4120 a         4287 c         3946 g         4146 t
ORIGIN      
        1 aggacaatca catccggatc gagaatattc acgacatgtg ccagcgattt tgccagccgc
       61 agctcgtagc gacgcaatgc cagttccgct accggatcgc tttcttcaac caggcggata
      121 atttcactgc ctttcagcgc atgtccgctc aaacgacgat aatccatcgc gaatcccgtg
      181 cccgaaataa aggtttcaat acaaccttgt ttaccgcaat aacaagggac ttcctcgcga
      241 taacgcagtt cgtcttcgtc catccacggt agcggattgt gtccccactc acctgccgtg
      301 ccattgccgc tgatatgcgc ccgcccattg aatgccacgc ccgcgccgca tcccgtgccg
      361 ataatcacgg caaataccgt ctgcgctccc gctgccgcgc catctactgc ttctgaaacc
      421 gccagacagt tagcgtcatt tgccagccgc acttcccgct gcaacctcgc gcttaagtct
      481 ttatcgaatg gctgaccgtt gagccaggtt gaattggcat tcttcaccac accggtgtaa
      541 ggcgaaattg agccaggaat gcccatacct accgttccgc gctgccccgt cgcctgctcc
      601 gccatatcaa ccaacgtggc gatcgtttca atagtctgcc ggtaatcatc acgcggcgtg
      661 ggcagacgat ggcggtacaa ctgctcccct gcatcgccca gtgcaatcac ttcagttttg
      721 gtgccgccta aatcgatacc tatacgcacg gtactctcct tatttttttc aatatcaata
      781 gcgtagagac ggacaaccgg attggcaatg caaggccgcc gacaattcgt tatcatgccc
      841 gctaaattta acaacaaggc cgtggaaatt atcatgctgt ggttcaaaaa tttaatggtt
      901 taccgtctta gccgcgagat ttcgctgcgt gcagaagaga tggaaaaaca gctagcctcg
      961 atggcattta ccccatgcgg cagccaggac atggcgaaga tgggctgggt tcctccgatg
     1021 ggatcgcaca gcgatgcgtt aacgcacgtt gccaatggtc aaattgttat ctgcgcgcgc
     1081 aaagaagaaa aaatcctccc gtctccggtg attaagcagg cgctggaagc gaaaatcgcc
     1141 aaactggaag cggaacaggc gcgtaagctg aagaaaaccg aaaaagattc gctgaaagac
     1201 gaagtgctgc actctctgtt gccgcgtgct ttcagccgtt ttagccagac aatgatgtgg
     1261 atcgacacgg ttaacggttt gattatggtg gactgcgcca gtgccaaaaa agcggaagat
     1321 acgctggcac tgctgcgtaa aagcctgggg tcgttacctg tggtaccatt gagcatggaa
     1381 aacccgattg aactgacgct gaccgaatgg gttcgctccg gtagcgcggc acagggcttc
     1441 cagctgcttg atgaagccga gctgaaatcg ttgctggaag atggcggcgt gatccgcgcg
     1501 aagaaacaag atctgaccag cgaagagatc accaatcaca tcgaagccgg aaaagtggtg
     1561 actaaactgg cgctcgactg gcagcagcgc attcagtttg tgatgtgcga cgatggttcg
     1621 ctcaagcgtc tgaagttttg cgacgagctg cgcgatcaaa acgaagatat cgaccgtgaa
     1681 gatttcgccc agcgttttga tgccgatttc atcctgatga ctggtgaact ggcagcgtta
     1741 attcaaaacc tgattgaagg attaggtggc gaagcacaac gttaattgct gattttcctt
     1801 taatgccgga tgcgacgcct gccgcgtctt atccggcgta cgaagccaca ccagggatat
     1861 aattattcgc tacggcggac aataattttt agcgcagcaa tattatgcgt tttatgctgt
     1921 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccagtgacgc gcttcactgc
     1981 gaattttccg cattgccccg gttttcacta aatcccgtaa cagcgaaaga tcgggttgtt
     2041 cgacaaataa gagtaatgat ggagcctctt ttaaccacgt cgttttcaca tcggcagcga
     2101 gctgtggatt attcatagct agcgaccgat gccgagactc ggtcaatgtc gttaacaacg
     2161 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
     2221 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaacaa
     2281 tatctggaga tcttcactga tatttatatc aaccttgatg aattatgaat atatcggaaa
     2341 ttgaaaaaat cgcagcaaag gaaatattat tatgggttaa agagaggata tttcagaaaa
     2401 atgccctccc gttcaagggg aaggcgagga attacagata gcggcacaga taagaggtgg
     2461 gttcggcaac ttgcagatga aactcactgt gaccgggaac attaaacacc gaaccggctt
     2521 catacacctg ccagtcggtc gcatcaggca gtaacacatt cagcgcgcca ctgattaccg
     2581 tcatctcttc cggctcagcg gtgctgaagg tgtattcgcc ttcaaccata acacccacgc
     2641 tggcgcgacc agtgctgctg ctggaaaagc cgattgattt cactttgccg gaaaagtact
     2701 cattactttg aagcataaac tggccctgtc tggtagttga acatctactt cactatgggg
     2761 gccagaggcg ctggctgtca cgcaaaatta caccagtaat tccgcagcca gccgtgcaat
     2821 caatacgtta gacagcaaga caggaacatc gagctgtttt tgcagtaaat cgcgatgacg
     2881 ttggtgaaat cctaaacaat ccagcatgat gacatcagcc ccttttgcca gtaattcttt
     2941 cccggcatca atgatttttt gttctgaatc atgaatgggg ttacccaatg aaaataccgg
     3001 cgatttctgc aaaatttgcc atttttgcgc ctgaacgggc agcatctcct caaccggaac
     3061 gataaccccc acctgatgat cttcaacaat agaggaaacc agtggaggca atattcgcga
     3121 cggctcaaga aagatcgtat tacgcgcagt catactacta atgtttgctg tactcattaa
     3181 taaaatgacg tcataaccct gattatcgag cacttcaacc acaccttgca ggtctcgctc
     3241 cactttacga cgcgaaacat gggccagctg gttgtcattt aataatgtga gaatggtgtc
     3301 ttcacctgct tctggcgcgt actccgccat cacttcttca cgacttaact tccccagcag
     3361 gctatgatgg gaaatattat cttcgtcaat gtattccgtc aggagcggca aaacctcctg
     3421 cataggtaca atgccgatgg tcaggatcgc caacgacgca ctcattgttc atccacctta
     3481 cttttctttc gtcgttaatt accgggcaag tgtgaagcac cactgctgac acttacttgt
     3541 gctacaaatg acaaaaagcg tagcagcaga cgtgcacggc ataatgtaaa gattccaaat
     3601 gattccagta atggattgtt attgtttaat attctaatta ttagaaaaac atgaattatg
     3661 aaaaatgtga cgcagatccc ttgtagtggc actcagaatc ccttccgagc agtctgatgc
     3721 ggtcactctt tgtcagggtc ctcatagcgt tttttcgcat ccatcgcttc ctgagcggtc
     3781 ggatgttcac tgatcaacga gtctggttta ggatgatcgg ctctgagttg gtaccaggtt
     3841 accgtctgtc ctggctttcc tttttcaatc gtcactatat atgcttcacg aggataaggc
     3901 ggtttcgttg gcatcgttct tccttatttc acggaatgaa cgttaagtat aggcgctcta
     3961 aaatcaacca ttgatcgtct gtgccagggc gctacgaatt tcagaaatca cctggctggg
     4021 ttcgtttgtt gcgtcgatga taatatgcgc aacttcgcga tatagcgcat cgcgttcttc
     4081 cagcacttcc tgaacttctt cgctcagcgg ttttcccgtt aaggttggcc gtaaatcttc
     4141 ttccggtgca gcttgcagtc ggttaaccag gactgatact ggcgcacaca aataaaccac
     4201 gatcccgtta ttttgcatga agtgacgatt aaattccgtc agaataatgc cgccgcctgt
     4261 agcgataacg gtggatggcg cagttaccgc ttccagcgcc gccgtttctc tggcgcgaaa
     4321 tcccgcccac tcttcccttt cgacgatctc cgcgaccgtc atattgagct gtgattgcaa
     4381 ccactgatcg gtatcgacaa accgacggtt aagcgaatcg gcaagggcca ttccgaccgt
     4441 tgttttacca cagccccgag gcccgatcag aaaaagaggt tgtgtcatcg tgggttttcc
     4501 ccaataggtc gcaatgcggc gaaagccggt gtcatgagaa tagcgatcat tccatcaaac
     4561 tagtacaatt tcgattgtaa agaaaaaatt ccacttaaag tgaaaatctc aatacatccc
     4621 ttactatacc aataaatatt caagaatgaa gtgtaaataa taaattacat ttagccacga
     4681 ctacgttgca cttccagcca ccacttctcc agctccgcgg caaaggcctg gcggtcacgt
     4741 tgtgaaaggc tatctggtcc gccggtctgg atcccactgg cacgtaaggt atccataaaa
     4801 tcgcgcatcg tcaggcgctc acgaatggtc gctggcgtgt aacgttctcc gcgcggatta
     4861 agcgccgctg cgcctttctc gatggcttca gcagccaaag gtatatctgc ggtgatcacc
     4921 aaatcgcccg cttcacactg ccggacaatt tcgttatcgg caacgtcgaa acctgccgcg
     4981 acgcgcagcg tacgaataaa tcgcgatggc ggcacgcgta aactctggtt tgctaccagt
     5041 accagcggca tctgcatacg ttccgccgcg cgatacaaaa tctctttaat tacattggga
     5101 cacgcgtcgg catccaccca aattgtcata aagtcatcct ttgttgggta atcctctatt
     5161 gtgtcgcgct tttgccttcc ggcatagttc tgtttatgct tctgccagcg attatcaaaa
     5221 caatgaattt cacggcagga gtgaggcaat ggaaaagaaa atcggtttta ttggctgcgg
     5281 caatatggga aaagccattc tcggcggtct gattgccagc ggtcaggtgc ttccagggca
     5341 aatctgggta tacaccccct ccccgaataa agtcgccgcc ctgcatgacc agttcggcat
     5401 caacgccgca gaatcggcgc aagaagtggc gcaaatcgcc gacatcattt ttgctgccgt
     5461 taaaccaggc atcatgatta aagtgcttag cgaaatcacc tccagcctga ataaagactc
     5521 tctggtcgtt tctattgctg caggtgtcac gctcgaccag cttgcccgcg cgctgggcca
     5581 tgaccggaaa attatccgcg ccatgccgaa cactcccgcg ctggttaatg ccgggatgac
     5641 ctccgtaacg ccaaacgcgc tggtaacccc agaagatacc gctgatgtgc tgaatatttt
     5701 ccgctgcttt ggcgaagcgg aagtaattgc tgagccgatg atccacccgg tagtcggtgt
     5761 gagcggttct tcgccagcct acgtatttat gtttatcgaa gcgatggccg acgccgccgt
     5821 gctgggcggg atgccacgcg cccaggcgta taaatttgcc gctcaggcgg taatgggttc
     5881 cgcaaaaatg gtgctggaaa cgggagaaca tccgggggca ctgaaagata tggtctgctc
     5941 accgggaggc accaccattg aagcggtacg cgtactggaa gagaaaggct tccgtgccgc
     6001 agtgatcgaa gcgatgacga agtgtatgga aaaatcagaa aaactcagca aatcctgatg
     6061 actttcgccg gacgtcaggc cgccacttcg gtgcggttac gtccggcttt ctttgctttg
     6121 taaagcgcca aatctgccga tttcaaccac tcacgatagt gactcatttg tgggttcagc
     6181 ggcgcaaccc ccacactaat ccgtaaagtt acctgtggcg tattcggcaa gcgtaacgtg
     6241 tttagccctt cacgcacccg taacatggcg gtaatggcgc tctcagctgg cgtaccggac
     6301 atgattactg caaactcatc gccgccaaac cgaccaatca catcgctacc gcgcagggta
     6361 atttgtaact gtcgggtaag cgccacaatc gcttcatcgc ccacatcatg accccaggta
     6421 tcgttgatgc tcttgaaatg gtcgatatcg ataatcagta acgttgcatc gcgattatgc
     6481 cgccgacagt tatcaaattc attgcgtaac atagtttccc aatgacgtcg gttatacacg
     6541 cccgtcatgc cgtcgcgggt actcatgacc tgcaacctgc gtttatgttc cgccagtttg
     6601 gttgccgtct ggtagctgac ccagccaaac agcagaggat aaacgacaat aatgggaagg
     6661 gagagccacc attccagcgg cgcactattg aacgacacgg taatgcccgt aagctcgagg
     6721 gtgacaaggc aggaaaccac catcaacacc agacccgcga caaacagacg ggggccgcct
     6781 gcccccatca aattcagaca cataatcatc aacatagcgg tggaaggcag cacgtttacg
     6841 cccattacgc ctacccacat tcccgctaat actgcatcgg tttttaagtt gtaaatttcc
     6901 cggctaagcg gatcgacggc cctgctcgct atctgccagg ctaaatgcgg ccagacgaac
     6961 gcccagccga ccaacaccag ccaccaccag cccggcggcg ggtgcgaaac cagcgttgaa
     7021 gcaatcggta agaacatgcc agccaggcca accgcacggg gtagtctgac gcgacgggcg
     7081 aagcgcagcc cggaccgctg atgttcgttt tgaggagtta aaggaggttc ctccccatgc
     7141 gccgccgctt ttttgaaaaa gttttcatca ttcattattt ttgggaacat tcagaaacaa
     7201 ttttcccaaa ttatagagac ggggctaaag gcagaagtat gaaatttcgg gtgagccact
     7261 ttcgtagctc acccgttagc actatcaggt atcaggcggc tttcttgagg caggcactca
     7321 taaacttatt acgatcatca cctttcagag attgttgcgt tgcttgatta ttacattcgc
     7381 gcatcttttg ctgctgtggc gtcaaacttt tttcaccagg cgcagacttg ctgttcttca
     7441 ggcaaccact catgtaggtc ttacgagcat cccctttcaa cgcctgcgcc gtcgcctgct
     7501 gattacagga ggtcatacgc tgttgttgtg gggttaacgt tctctcggca gcgccgactg
     7561 tggttaaaaa aaccagaccg aaaagcaagg taaccagtaa tgtaattttc atagcaccat
     7621 ccctcttcat gttttaacca tgagcgtatg cgcccgagat ttgccattaa gtctggttgc
     7681 taacagcaaa aaaaccaccc ggcagcgaaa attcactgcc gggcgcggtt ttatttcagc
     7741 cccagggcgg ctttcatggt gtagaagaga tcggtctggt cggtcagtcc aacgacattg
     7801 gcggcatgcg ggccatacgc cgcaatacgc aactgactgc cggtatgttc ttgtgaatcc
     7861 tcttcggagt tcccgtagct catcaccatc actgcgccat ctttggtatt tagcgcctgg
     7921 gtgaggcccg gagctttggt atccggcgcg acactctggc tggcgtgggc gtgatcagcg
     7981 gtgactatga ccagcgtgtt gccatccttt ttagcgaatt ccagcgcccg ttgtacggct
     8041 tcatcgagat cgaccgtctc gccaatttgc ccacaaggat tcgcagcgtg atcctgttta
     8101 tcgatcgacg caccttcaac ttgcaggaaa aatcctttct catttttact caacaattca
     8161 atggctttgt cggtcatctg cgccagggtt ggtacactgt cattacgttg cggattaggc
     8221 gtacaggtga ctgcgggctt atcgatattg ccgtggtacg ttgctttcgg tcctagccag
     8281 cgcactggca tattgccgtc agcaaacagt ccaagcagag gtttttgctg attcgcttcc
     8341 gtcaccgcat ttagcgaggc ggcatcgctg accaactgat aaccacgcgc ctgtgcctgt
     8401 tcacgcagcg tttttccctg ccattcaccg gcggttgccg tttcagcaaa ggtttttgcg
     8461 ccaccgccaa gcgtaacgtc ggcacgagca ttaagcagct gttcggtaat cgatcctttt
     8521 ccgccttttt ccagagcgtt acccggacat ttttcactgg tcgcgctcgg accgtagcat
     8581 ttgcgcgaag tcacatgtgc caccagcgca gcgggcgtgg catcctgcaa ctctgcggta
     8641 gaaacgttac cggtcgccag acctgcggct tttgccattt ccagaatcgt tgggtgatct
     8701 ttttcgtgaa tatcgacgcc cagcgcgccg ttataggttt tgacaccggt tgaccaggcg
     8761 gttgctgatg cagccgaatc ggtgacgtag tccggtttgc cggttttttt attcagcgca
     8821 tagtgagtgt attgcccggt aagcggtaag gcatcgatac ctttaaaaaa gccgcccgca
     8881 ccttcggcat aattacgtgc ggctgtaatt tccgaatccc ccatcccatc gccaatcagc
     8941 aaaataatat tttttgcagg tttatcgcta agagaatcac gcagagcggc agtctgatca
     9001 cccgttaaac ggcgagcacc gccgggtgca gtaatatcgc cctgagcagc ccggttttcc
     9061 agaacaggca tttctggtgt ccgggctttt gtcacagggg taaacagtaa cggtaagagt
     9121 gccagtgcaa tagtgctttg tttcacttta ttttctccat gtacaaatac attaaaaaat
     9181 aaaaacaaag cgactataag tctcggccgt gacaacttta tgacagctgt tgaaaagatt
     9241 aactttttac tgacgaggat gcttcaataa cttctttaca taattgcgca gcagctccgt
     9301 atcgtcgtca ggaatgctgg catcaggctt tacctcgtaa agagccccct ccacctgttc
     9361 aatcaagcgc tgttggtcat tttgtgccat attgcgaagc attgcagtga cgataatctc
     9421 caaagcttct acctgcgcgc acagttcttt cgactcttct tctttttggg caagctttaa
     9481 taacaactca gcaatgagat ttttcatgtc tgtatttcct tatccaacgt atggagaagt
     9541 taacattatg tttcacaact tcatagtgtc tggaagtatt atttttatga caaaggaaat
     9601 actttgcgca aatgttcttt gtttgattac cagcgaaacg gttctcatat ttaaataatt
     9661 aaccacacaa agaacaggcc tttaattatc aacctttatt ataaacaaaa gttatgaaat
     9721 atattctctg cgtttactta caactaagag aaaaaactca ccgcatggcc atttaagcca
     9781 ggaataaatt agcttcccgt tcatcatttt tgtgatctcg cgcacatctt tgtgataacg
     9841 cactttcttc accattacgt tgcacgcttt gtcagcagaa gattgcaaca ttctgtcaga
     9901 aggccgcgaa tcccccgttt tgtcgtggta atgtatgcac tttgtgttag acgggatagg
     9961 tttttaagat ggaaaaactg cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg
    10021 aagtgtctct gcaatcggca aaaaacattg tcgatgccat tgataaaagt cgcttcgacg
    10081 ttgtgctgct gggcattgat aaacaagggc aatggcacgt cagcgatgcc agcaattatc
    10141 tgctaaatgc agacgatcct gcccatattg cgttgcgccc ttcggcgact agccttgcgc
    10201 aggtgccaag taaacatgag catcagctta tcgacgcgca aaacggtcag ccgttaccga
    10261 cggtggatgt cattttcccg attgtccacg gtacgctggg cgaagatggt tccttgcagg
    10321 gaatgctgcg ggtcgccaat ttaccgtttg taggttctga tgttctggct tcagcagcct
    10381 gtatggataa agatgttacc aaacgtctgc tgcgcgatgc tgggctgaac attgcgccat
    10441 ttattaccct gacgcgcgcc aatcgtcaca acattagttt tgccgaagtg gagtctaaac
    10501 tggggttacc actgtttgta aaaccggcta atcagggctc ttctgttggt gtcagcaaag
    10561 taaccagtga agaacagtac gcaattgccg tcgatctggc gttcgaattc gatcataaag
    10621 tgatcgttga gcaagggatc aaaggtcgtg agattgaatg cgcagttctg ggcaacgaca
    10681 acccgcaagc cagcacctgt ggcgagatcg tactcaccag cgacttctat gcctacgaca
    10741 ccaagtacat tgacgaagat ggcgcgaaag tggtagttcc ggcagccatt gcgccagaaa
    10801 tcaacgataa gatccgggcg attgccgttc aggcttatca aacgttggga tgcgcaggca
    10861 tggcgcgtgt agacgtgttt ttaaccccag agaacgaagt ggtgatcaac gagatcaaca
    10921 cactgcctgg cttcaccaac atcagtatgt atccgaagct gtggcaagcc agcggtctgg
    10981 gttacaccga tctgatcaca cgtttgattg agctggcgct ggagcgtcac gctgcggata
    11041 acgcattgaa aaccacaatg taatatttta acgcccgata atattcttat tgggcgtttt
    11101 cagtttcttc atcgcgacga cgaataatca accccgccag ccagaaacta atcacccacg
    11161 tcaccagacc gacggcatag gtctgccaac cttttgcttc aaatcccagt aaccccacca
    11221 cgccattaag aatgaatata aggccaatgg cgaacgcata gtagtgccag tcacggcgga
    11281 tttttacagg cagattcatg gcagctccgt taacgaaaaa caacatcctt gcatattctg
    11341 caacaaccgt ctgtggtgta gacaacattt tcgtaattgt taataaattt aacttaaggt
    11401 tgccattttg tgactcacaa cgaatattca cgcttcggga atattcccaa catcaattta
    11461 ccatgattct gatattgaca atctaaatga cctgtcagac ttgccataag ccgctggtat
    11521 cacaccagaa gagaagcaga tgacttccgg aggtttttat ggctgatttc accctgtcaa
    11581 aatcgctgtt tagcggaaaa tatcgcaatg cctcttcaac gcctggcaac attgcctatg
    11641 cgttgtttgt gctgttttgc ttttgggctg gggcgcaatt gctgaacctg ttagtgcatg
    11701 cgcccggcgt ctatgagcgt ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa
    11761 ttggtttagg tgtcggcacc attttcgggc tgatcccgtt tttagtaggc tgcctcattt
    11821 ttgcagtggt ggcgctatgg ctgcactggc gacatcgccg ccagtaagat ttacgttgcc
    11881 agatacggcc taaaacgcct tatctggcat gacattcctg tttgatcact gtttcataca
    11941 acgcgcccgc cgttcgtcta cgcgcttcgc aaaccaggcc gtagtcaggt tgcgggtgat
    12001 tttcgggctt tccagttgaa tgccaggcaa catttcgcgg ggtagtgatt tacccgtttt
    12061 cgcttcggca agttgataaa ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc
    12121 acctttctct aactgacggc gaatttcgct gtcgctcatc cccagttttc ccgccagttt
    12181 gcgcgttgcc agttcggttt tcccgggttc tttactgtcg taacgaatta aatcaccgtc
    12241 cagtgccagc ttcacgccgc tggccttact gaccgcgttc tgaaacgcgg cattacggct
    12301 ggcgtaccag ccagcgttaa aatcagcaaa gcggtatatc ggtgcgctat agctggcggg
    12361 ataattcagt aaatggtaag taccaaacca caacccgccg cggcgactga agacttcctg
    12421 acgaactgta ccgtccattt tccacggata ccctttggta tgctgttcgg caaaagcaat
    12481 gctgacctgc atcggcccac cggtgcgcac cggattaagc gaaccaaaca gcgtctgccc
    12541 cattggtacc atgctgatca gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac
    12601 cgaatctaac cgttcgctat aacttttgcc gtttggcgat ttgattttta gcgcggtatg
    12661 aaccagaaaa gcaggaatgt gcatccgttc agcacgacgg tcaatctctt gccaggcgat
    12721 cttgcttaag tccggaacgg ccggatctgc ctgataattt gactcctgct gcgccaccgc
    12781 cagcaccgag cagacgtttt ccagcgtcgg tgccagcccc tgactttcaa aggtggtggc
    12841 gagatctttc gcccaggcat cgcggtcttt cacgctcgca ggcatcttct gccgcacgac
    12901 gctcgctaca tctatcgctt tttcgccctt tttcaacggt tgcggagcct gactactaca
    12961 ccccgctaaa accagaaccg ccaacaatga cagcgaagat agatgattga cgcgcgacat
    13021 tacttctcct ttttagctca aggtatgggt tacttcctga attttgtcac catccagctc
    13081 atgttcaaag ctgcgcagac gtttgtagat agacatcaac tcaaccagtg tggtccatga
    13141 gttaatcagg tactggaaag caccgcgaac ctgaccaaaa acgttggtaa tctgcgtcat
    13201 caggccgagc gtaatcgtac cggcaacaat tgacggaaac agcaagaaca aaccgaaaac
    13261 gttatcgacc tgcaaataga gaatgcgggc gatgttgaaa tacatatagt gaaaatagag
    13321 gcgaaaatag tttttccgta cggcgctaaa cagctcgcgt accgtaggcg gcgtcgcgcg
    13381 cgtggcatcg tcttcaccat aaaccagctc tttacggtag gcggcctcta ctcgctggtt
    13441 tttaaactcc agccccggca gtttgatccc taccactgcc agcaatccgg tccccatcag
    13501 cgaccagacg atagcggcaa tcaccagacc atacggaatg tgcccgataa tcggcagctc
    13561 cggcacatgc gcggagagcg ttaccagcac cggcaggaag gcgaccaacg tcatgatggc
    13621 gttgataaaa ctgacgccca tattctccag cgttgaagca aaacgcatgg tgtcttcctg
    13681 cacacgctgt gcggcacctt cgatatgacg cagttgttgc cagttcgcca tgtaatattc
    13741 gttcatcgcc gtacgccagc ggaacacata gtgactgaca aagaagttgt tcagtacact
    13801 gatcaccacc gcgatcagcg caatccccag aaagacgccc acttcgcggt aaaattgttc
    13861 gatagtgact ttatgcggcg aacttagcgc ggtttgaatc agatcataga acggtgcata
    13921 ccaggcgttg acggcgaccc cgacttccac caaaaaccag gtgacgaaga tgatcagtgc
    13981 agtaccgagt attgaccagt attgccaacg gtgcgggctg tagataaacc agaaaaatgc
    14041 aaaaagtcct acgcaaacaa tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa
    14101 acgcgcggcg ctaatcggga tctgcccgga agcgccggtg atacgcgcca cccagtctcc
    14161 cccaccggct tgccagaaga taacggcaat caatgcccaa acaaaggccg agagaaaaaa
    14221 cgctcccggc tttgggaaaa aagacttaaa catatgcact cctgctaact tcttatcgtt
    14281 ttaacaactg ctgtgtatta accgcatgac cactacaaag cgtcagccaa atgaaaccgc
    14341 ccattgtgac ccatagcaag gcgcttagtt cgccagcctg accataagaa ttgtttcgac
    14401 ggatttcaca aaccaaagat tgtttacata agcggattaa cacgcgcagg atcacaaatg
    14461 caggcattgg tgagcaggaa aaagctcgtg tttacgcgcc gttacgtttt taacactatt
    14521 attgcactaa ttcgcccttt gcaatccatc aatgagtagt ataaatacgc tcagttacct
    14581 tcattcaatc tatggacacc accgttgaaa cgtagtctgc ttttttctgc cgtgctgtgt
    14641 gcggcgtcat tgacctccgt ccatgcggcg cagccgatca ctgaaccgga gtttgcctct
    14701 gatattgtcg atcgttatgc cgatcatatt ttttacggca gcggtgccac ggggatggcg
    14761 ctggttgtta tcgacggtaa ccagcgcgtc tttcgcagtt atggcgaaac gcgacctggt
    14821 aataacgttc gcccgcagct cgattcggtc gtgcgtattg cttccctcac caagctaatg
    14881 accagtgaaa tgctggtgaa attgctcgac caggggaccg tgaagttaaa cgatccgcta
    14941 agtaaatatg ctccgccagg cgcacgcgtg ccaacctaca atgggacgcc cattacccta
    15001 gtgaatctgg caacccatac cagcgccctg ccccgtgaac agcccggtgg cgcggcacat
    15061 cgtccggtgt ttgtctggcc aacgcgcgag caacgctgga aatacctttc tacggcgaag
    15121 ctgaaagccg cgccaggcag tcaggcggcg tactctaacc ttgcgttcga cctgctggca
    15181 gatgcactgg cgaatgcgtc cggcaagcct tatacccagt tgtttgaaga gcagattacc
    15241 cgtccattgg ggatgaaaga caccacctac accccctcac cggatcagtg ccgccgttta
    15301 atggttgctg agcgcggtgc cagtccgtgc aataacacgc tggcggcaat tggcagcggc
    15361 ggcgtctatt ccacgcctgg cgatatgatg cgctggatgc agcagtattt atcatctgat
    15421 ttttatcagc gcagtaacca ggcagatcgc atgcagacgt tgatttatca gcgcgcgcag
    15481 tttaccaaag tgattggcat ggatgttccc ggcaaagcgg atgcgcttgg tttaggctgg
    15541 gtatatatgg ccccgaaaga gggtcgtccg gggattattc agaagacagg cggtggtggc
    15601 ggtttcatta cctatatggc gatgatcccg cagaagaata ttggcgcgtt tgtggtggtc
    15661 actcgttcgc cgttgacgcg ctttaaaaat atgagcgatg gcatcaatga tctggtgacc
    15721 gagctaagcg ggaataaacc gttggttatc cctgcatcct gattaatact ctgaaggcaa
    15781 acggttgata gcgaccagtt tgcctttatt catttcgata tagccgcctt tacgcaaagc
    15841 agcgagaact tctgcgacga cagaacgcga tatgcgtgta cgttgatgga tataattcat
    15901 cacaccaata cgtgatcgca attcttcatt ccagtcaatc attgataata atgttgcgcg
    15961 gatttgttcg taggaattat gcccaatgag ctgcacgtcg cgtaattcca gaattctatt
    16021 ttgccaggct aaccagtaaa aagcgtctcg ccagagttga ttttgttcaa taagcgtaat
    16081 ggtttgtttg gctggtagat gatatcccgt acaatttcct tctgatatta atttgtatgg
    16141 tatatcgttt ttcattaaac catcagccag ccccataata taagaagcct gggtaatacc
    16201 gataagtacg ttttcttctc tacgcagaga gataacgccc tctaaaataa cgaaagtatc
    16261 ttcgctattg acatcactgg aaaatataac ttgcttttca ttattaaact cgaagcgcgt
    16321 accgtatctg gacaaacatt tatcgagctt accaaattcc tgaagaggtt taactacaga
    16381 taacatttgc gcgtcctttg cagtaatgcc cgtcaaatcc ttgacgggca ttatttagat
    16441 taaattacca gaatttcttc ggagtgaaga atattaccaa gtatatttaa cacccacgt
//
LOCUS       DAJTNA010000084        16476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTNA010000084 DAJTNA010000000
VERSION     DAJTNA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16476
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.085"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..1561)
                     /locus_tag="OO161_002300"
     CDS             complement(<1..1561)
                     /locus_tag="OO161_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5463346.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFNMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVLYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVL"
     gene            complement(1579..3495)
                     /gene="mngA"
                     /locus_tag="OO161_002301"
     CDS             complement(1579..3495)
                     /gene="mngA"
                     /locus_tag="OO161_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5463347.1"
                     /translation="MNLTTLTHRDALRLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKQALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            3664..4386
                     /gene="mngR"
                     /locus_tag="OO161_002302"
     CDS             3664..4386
                     /gene="mngR"
                     /locus_tag="OO161_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5463348.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFLDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(4490..5359)
                     /gene="sucD"
                     /locus_tag="OO161_002303"
     CDS             complement(4490..5359)
                     /gene="sucD"
                     /locus_tag="OO161_002303"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5463349.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(5359..6525)
                     /gene="sucC"
                     /locus_tag="OO161_002304"
     CDS             complement(5359..6525)
                     /gene="sucC"
                     /locus_tag="OO161_002304"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5463350.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(6801..8018)
                     /gene="odhB"
                     /locus_tag="OO161_002305"
     CDS             complement(6801..8018)
                     /gene="odhB"
                     /locus_tag="OO161_002305"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5463351.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLEKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(8033..10834)
                     /gene="sucA"
                     /locus_tag="OO161_002306"
     CDS             complement(8033..10834)
                     /gene="sucA"
                     /locus_tag="OO161_002306"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5463352.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(11135..11851)
                     /gene="sdhB"
                     /locus_tag="OO161_002307"
     CDS             complement(11135..11851)
                     /gene="sdhB"
                     /locus_tag="OO161_002307"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5463353.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(11867..13633)
                     /gene="sdhA"
                     /locus_tag="OO161_002308"
     CDS             complement(11867..13633)
                     /gene="sdhA"
                     /locus_tag="OO161_002308"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5463354.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(13633..13980)
                     /gene="sdhD"
                     /locus_tag="OO161_002309"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(13633..13980)
                     /gene="sdhD"
                     /locus_tag="OO161_002309"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5463355.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(13974..14378)
                     /gene="sdhC"
                     /locus_tag="OO161_002310"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(13974..14378)
                     /gene="sdhC"
                     /locus_tag="OO161_002310"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5463356.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            14759..14866
                     /locus_tag="OO161_002311"
     CDS             14759..14866
                     /locus_tag="OO161_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463357.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            15072..16355
                     /gene="gltA"
                     /locus_tag="OO161_002312"
     CDS             15072..16355
                     /gene="gltA"
                     /locus_tag="OO161_002312"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5463358.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         3901 a         4485 c         4132 g         3958 t
ORIGIN      
        1 gcaatactcg gtcatagcgc acaccgctgt ctttatctac cagttgcaga gaaccatcct
       61 cattgagcgc aatttgccag aaagcatttt ccagtatccc ttcagcgtca ctttttgccg
      121 caatcacgtt gccaggctga ttcgcttcga tataaagcgt gcgatagccc atagaaggga
      181 caatctggtt gatctgtata tcaaactcca taaagggatc gtaattaccg taatgaacta
      241 tttgccgatc gattaggcct ggatcgatct cacgggcatg gcgaataaaa tacagtacag
      301 gctgaccgcg atcgtctcgt agattaaact ggctggcgcg cagccgcaca gtggtgttaa
      361 taacttcttc ccgcggccag ggcatcaggt taaacagcac gagtttgtcg gcatcactct
      421 gtggcatgtt atcggcaatt ttgcgcatgt tgaaacgcag cagattatcc gccatgtctt
      481 cagccagttc gaagcgggcg acaatttcgc gatgaacttt gtcactgcaa caacaaccga
      541 tactgtcgtg ggcatgattt tttaagatct ctttccacat tttctccagc aagccgtggt
      601 ggtattcaaa acccaacgtc caggccagcg ttgccagcgg ttccagcaga ttaacaatct
      661 tattttcaat acgtgcgtgg gcaattttga tatccatgcg cgtggaaccg atggtgcgat
      721 gtacgcgcat atatttgcca tcgataaatt cacctttcag ggtggccaga ctttcacgct
      781 gcgcttcgat cttctcaaat acctcttcaa agcggctcat cacaaactta cgttgagggt
      841 agatctcacg tagcttatcc atcacttcga agatattttg ctgcaatggc atctggtcat
      901 gaccattcgg cagcaaaatc tctttggtta cagacgcttt ttccagcacg tcaaaataac
      961 tgtcgaggcg tttacgtaat ccgttttcgt cggcaggtaa gtacttaccg atggcgtagc
     1021 ccagcggcag cacctgcgcc gtcacttcgc taccgtcact gctttgccac aaaaactcgg
     1081 ttttatcagt accgtggcgc tccgaacatc cgcgccagaa catggtgcgg gtaatgccaa
     1141 atccattgta gatatgcggc agttgcccgg acatgccaaa ggaatctggt aaataaccta
     1201 ttttcatcgg ctcgccaaac gcgaggcagt cacgcattcc gtacatcaga ttacggacga
     1261 tggattccgc agaaacaatc gtggtatcgg tctgggtata ccaggggccg ataatcaact
     1321 tgccggcttc tacctgtttc ttcacacggt ctttgttttc cggtttcacc gcgaaataat
     1381 cttcgaggat cgccgtttgc ccgtcgagta cgtaatattt gtattcgttg tcctgttcca
     1441 gtcggcacag gatctcttcc atattattga ccagcagaat acgtgactct tcggtggtga
     1501 aataccactc tcgatcccag tgcatatgcg gggtgatgtg aacgcgagat accgctttca
     1561 tcgtcgtttc ctttttgttt atggcattac gccatcagtc agatagttgc catgcttaac
     1621 cgcgtgacgc cgccagatca ggagaattgc agtcgagatt gcagccccca ccaatgccgc
     1681 accaaaccag ccaattgcag ccataacacc gcccgcgcca ttatcatgaa gtaaaaagag
     1741 cgagaaaatg ccggcaccgg gtgtcgaaag gccgatattc atcgcaccga caatagcgcc
     1801 cgttaccata gagcccagca caaacgaacc aataacccgc agcggatctt caatcgccat
     1861 cgggatcgcc ccttcggtaa tacctgccag ccctaacagc caggtggatt tcccggtctc
     1921 aatttcaaac tctttaaaca ggcgcggtgc gagcatcgtg gaagcggtta cggtaaatgc
     1981 cgaaaccatt ttgacggagg cgaaaatggc atacgggccg taaacgccgt tcgccattgc
     2041 gcccaggcag aatgcataag cggctttgtt cactggccct ccaaggtcaa aggaacacat
     2101 aaaaccgaaa atggcaccca gcaacagcgc gttacttcct gacagaccgt tcagccaggc
     2161 ggtaagcgag ttattgatcc atgcgacagg ttctcccacc acaaacagca tcagactgcc
     2221 cgctcccaac gtaccgagca ccgggtagag ataaaaagtc aggaatccat tgaatttact
     2281 gctaagacgc aagtgatttt tcacccagcg catcaagtaa ccggctatca atccgccaac
     2341 gaccgcgccg agaaacccgg agccgatcat gttggcggca agtccagccg caaagcctgg
     2401 cgctaacgcc tgtttatctg ccagagaata ggcggtatag gccgcgagca ccggtaccat
     2461 caaaattccg agcagcccgc cgcccagctt gcggtacatc cacagccagg agttttcttc
     2521 attaaacaga tcttgtagcc cgaagatttg cgacagtaat accgcgaccg ccagcaccgt
     2581 gccccccgcg acaatcaacg gtacggcaaa agagattccg ctcaacagtg cctgtttcag
     2641 ctccgttttg acacttttca ccggttgcgt atcttgctgt acggtacgcg tctcatcgct
     2701 acgcttgagg gtaagcgctt gttggatcaa cgcttctgca tggcgaatcg gctcggcaac
     2761 aggcactgaa agcgcgggaa tgccattaaa acgctcactc tccttgatgg cgacttcagc
     2821 cgcaaaaata caggcggttg cactattgag ttgatccgcc gttaaacgcc cttcaatgcc
     2881 gttagcgcct tgtttttcaa cgtaaacatt tacgccgagt ttgcgtccgg ctttttccag
     2941 atattccgca gccatatagg tgtgagcaat acccgccgga caggccgtta cgcagacgat
     3001 agttggcgcg ttggaaaaag aggcagaagg ttgcgtgcct cccttgtcat ccagcgccga
     3061 gagcaactca tcaggcgtcg tcgccgactg tatacgcgcc cgaatctcat catccgcaag
     3121 gcgcgtagtc agcgctgtca gcagttgcat atgcatagta cccgcttcat tggggggaat
     3181 cgccagcagc accactaaat caactgcttc cgggccatca acgccttccc actgaagcgg
     3241 ctcgctgagt gtggcgaccg caaacgccgc ttcttttacc gcagcagttt tgccatgcgg
     3301 cacagccaac ccttcaccta aggcggtcgg gccaaggctt tcacggcgat acacttcttc
     3361 cagaaattgc tcagtactgg aaattttccc cagagcagca agacgttgag tcaacgcgtg
     3421 gatggcctct tcacggctgg taaagcgcgc attcagacgc aacgcatcgc ggtgggtcag
     3481 agtcgtcaga ttcatcatga tcctcacttg atcgttttta tagtcgcgcc agtgtgcccg
     3541 atgaaacaat accattatat gatcaatttc acttgattga aatcatttgt attgtatttg
     3601 cattatttac aggatgaatt tatcgggcat aatggctaaa ttcgtattaa tgagatacta
     3661 aaaatgggac acaagccctt ataccggcag attgccgatc gcattcgtga acaaattgcg
     3721 cgtggcgagc ttaaacctgg cgatgcgtta cccactgaat cagcgctaca aacagagttt
     3781 ggcgtcagcc gggttacggt gcgtcaggct ttgcgccagc tagtcgagca gcagatcctc
     3841 gaaagcattc agggcagcgg gacttacgtc aaagaagagc gggtcaatta cgatattttt
     3901 cagttaacca gttttgatga aaaactgtcg gaccgtcacg tcgatacaca cagtgaagtc
     3961 ctgatatttg aagtgattcc ggctgacgat tttcttcagc aacagctaca aatcaccgcg
     4021 caggatcgtg tgtggcatgt gaagcgcgtg cgctatcgca agcaaaagcc aatggcgctg
     4081 gaagaaacct ggatgccgct tgcgttgttc ctggatctca cctggcaggt catggaaaat
     4141 tcgaaatatc actttattga agaagtgaag aagatggtta tcgatcgtag cgaacaggaa
     4201 atcattcctc tgatgccaac cgaagagatg agccgtctgc ttaatatcag ccagacaaag
     4261 ccgattcttg aaaaagtatc gcgtggatat ttagttgatg gtcgggtgtt tgaatatagc
     4321 cgcaacgctt ttaataccga tgactataaa tttaccctga tagcgcagcg aaaatcatcg
     4381 cgataagcac aaaaaaggcc atcatacgat ggccaaccat gtcgaaaccg acagcccttc
     4441 gccattcctg tagagggaag cggcgagggc tatttcttat tacagatatt tatttcagaa
     4501 cagttttcag tgcttcaccg atatccgcca ggctgcgaac ggttttcacg cctgcggctt
     4561 ccagagcagc gaatttctca tccgcagtcc ctttcccacc ggcaatgatc gcacccgcgt
     4621 ggcccatacg tttgcctttc ggcgcagtca caccagcgat gtaacctaca actggcttgg
     4681 taacgtgctc tttgatgtac gctgccgctt cttcttcagc gctaccgccg atctcaccga
     4741 tcatcacgat cgcttcggtc tgcggatctt tttcgaacat ttcgaggatg tcgataaagt
     4801 tagagcctgg gatcgggtca ccgccgatac caacacaggt cgactgaccg aaaccgtaat
     4861 ccgtggtctg tttaaccgct tcataggtca gtgtaccgga acgggaaacg atacccactt
     4921 tacccggttt gtgaatgtga cccggctgga taccgatttt gcattcaccc ggagtgataa
     4981 cgcccgggca gttcgggccg atcatacgaa cgccggcttc gtccagtttc actttcacag
     5041 tcagcatatc cagcgtcggg atgccttcag tgatggtgat aatcagtttg atgcctgcat
     5101 caatagcttc cagaatggag tctttgcaga acggtgctgg tacgtagata acagaagcgg
     5161 tagcgccagt agcagcaaca gcttcacgca cggtgttgaa caccggcagg ccgaggtggg
     5221 tagtaccgcc tttacctggg gttacgccgc caaccatttt agtgccgtat gcaatggcct
     5281 gttctgagtg gaaagtcccc tggctaccag taaagccctg gcagataacc ttggtgtttt
     5341 tatcaattaa aatggacatt atttcccctc cactgcggca acaacctgct gagctgcatc
     5401 cgtcagacct tttgctgcaa taatattcag gccgctgtca gccagtttct tcgcgccgag
     5461 ttcggcgttg ttaccttcca gacgtaccac gaccggtacg ttaacaccca cttctgctac
     5521 cgcgccgatg ataccgtcag cgatcaggtc gcaacgaacg ataccgccga agatgttaac
     5581 cagaacgact ttcactttgt cgtcagagag gatgattttg aacgcttcgg ttacacgttc
     5641 tttggttgcg ccgccgccaa cgtcaaggaa gttagccggt tcgccgccgt gcagtttaac
     5701 gatgtccatc gtacccatcg ccagacctgc gccgttaacc atacaaccga tgttaccgtc
     5761 cagcgcaacg tagttcagtt cccactgtgc agcctgtgct tcacgcggat cttcctgcga
     5821 ctggtcacgc atttcgcgca gatcaagctg gcggaacagt gcgttgccat cagcgcccag
     5881 tttgccgtcg aggcaaatca gatcaccctg tttggtgatg accagcgggt tgatttcgat
     5941 caacgccagg tcgcgctcca ggaaaatggt cgccagaccc atgaagattt tggtgaactg
     6001 ctgaaccagt ttaccttcca gacccagttt gaacgccagc tcgcgtccct gatacggcat
     6061 cgggcctgtc agcggatcaa gcgcaacttt atggatcagc tgcggagttt cttccgccac
     6121 tttttcgatt tccacgccgc cttcagtgga ggccataaag accacacgac gggaactacg
     6181 gtctacaacg gcaccgagat acagctcttt agcgatatcg gtcgctgctt caaccagaat
     6241 ctggttaacc ggttggccat tggcatctgt ttgatacgtt accagacgct tgccgagcca
     6301 attttctgca aaagcacgga tgtcttcttt gctgtttaca actttcacac cgcccgcttt
     6361 accgcggcca ccagcgtgaa cctgacattt cactacccac ggaccggcac cgatttttga
     6421 agcggcttct tctgcttcgc gcggagtagt acaggcataa cccaccggtg ctggtaagcc
     6481 atagcgggca aaaagttgtt ttgcctgata ttcatgtaag ttcatgtgtt ctgtccatcc
     6541 ttcagtaatc gttatctttt aaaccgtaga ccgaatattt cgtatcgctt ccgggcattg
     6601 tcggtcacct gtaggcctga taagacgcgt aagtgtcgca tcaggcatcg cttgtcggat
     6661 gcggcgcaag cgccttatcc gacctacatt ggtgcttccc gtaggcctga taagacgcgt
     6721 taagcgtcgc atcaggcttc aattgccgga gaaggcgata atgccttatc ctgtctacag
     6781 tgcaggtgaa acttaaacta ctacacgtcc agcagcagac gcgtcggatc ttccagcaac
     6841 tctttgatcg ttaccaggaa gcccacggat tcgcgaccat cgatcagacg gtgatcgtag
     6901 gacagcgcca ggtacatcat cggcaggatc tcaacctgac cattcaccgc catcggacga
     6961 tctttgatag cgtgcatacc cagaattgcg ctctgcggcg ggttgatgat cggcgtagac
     7021 atcagggaac cgaacacacc accgttggtg atggtgaagt taccaccggt cagatcttca
     7081 acggtcagct tgccatcacg gcctttgact gccagctctt tgattttctt ctcgatgtct
     7141 gccatgccga gggtatcgac atcacgcaga accggcgtca ccaggccgcg cggcgtagaa
     7201 accgccatgc tgacgtcgaa atagttgtgg taaaccacgt catcgccgtc gatagaagca
     7261 ttcacttccg ggtaacgttt cagggcttca accaccgctt tcacgtagaa ggacataaag
     7321 cccagacgga tgccgtggcg tttttcaaac gcttcaccgt actgcttacg cagatccata
     7381 atcggcttca tgttgacttc gttgaacgtg gtcagcatgg cggtggagtt tttcgcttcc
     7441 agcagacgct ctgccacacg cttacgcagg cgagtcatcg ggacacgttt ttcactacgt
     7501 gcagccagag ccggttgcgc cgccggagca gccgctgccg gtgcagactc tttcgccggg
     7561 gatttctcca gatgtttttc cacatcttca cgggtcagac gaccacccac gccggtgcct
     7621 ttaatagcgc tggcgtcgag gttatgttca gccagcaagc gacggatcgc cgggcttaac
     7681 gcatcgttgt tttgctcttc cagagacgcc tgctggcgtt gcgccggagt ggacgctttc
     7741 tcttcagatt tggcgctggt ttctttaccg gcgctgttgc cttcacgcag gcgaccaagg
     7801 atctgacgag acgttacggt tgtaccttca tcttccagaa ccgcatccag aatgccgtct
     7861 gctgatgccg gtacttccag taccactttg tcagtttcga tttctaccag cacttcatca
     7921 cgtacgactg cgtcgccggg ttttttatgc caggttgcga cggtggcatc ggctacggat
     7981 tcaggcaggt cagggaccag aatatctacg ctactcattg tgtatccttt atttattcga
     8041 cgttcagcgc gtcattaacc agatcttgtt gctgtttctg gtgaacggac atatacccta
     8101 ccgccggaga ggcggaggcc gggcggcctg cataacgcag agaagcccca aacggaatca
     8161 cttcacggaa atgatgctgg ctgcagtacc atgcgccctg gttgagcggc tcttcctggc
     8221 accagacaaa atccttgacg tgagcaaact gctgcaacac ttcctgcatc gctttatgcg
     8281 ggaacgggta gagttgctcg atacgcacaa tggcgacatc gtgttgattg ttcttacgac
     8341 gctgttccag caggtcgtaa taaaccttac cagaacacat cactacgcgc ttcacgccct
     8401 tcggatcaag ctcgtcgatt tcaccgatgg ctggcaggaa ggtgccgttc gccagttctt
     8461 cgaggctgga aaccgccagc ggatgacgca gcagggattt cggcgacatc acgaccagcg
     8521 gacgacgcat cccgcgcagc gcctgacgac gaagcatgtg gtaaacctgt gccggggtag
     8581 acggtacgca cacctgcatg ttttgctcag cacaaagttg cagataacgt tccagacgcg
     8641 cggaggagtg ctccggcccc tgcccttcat aaccgtgcgg cagcaacatc accagaccac
     8701 acatccggcc ccatttctgt tcgccagagg agatgaactg gtcgataacc acctgagcac
     8761 cgttagcgaa gtcaccgaac tgcgcttccc agatggtcag agtgcgtggt tctgcggtgg
     8821 cataaccgta ttcaaacgcc agcactgctt cttcagacag tacggagtcc cagacgcgga
     8881 acgcgccctg cccgttatgg atgtgttgca gcggcgtgta agtggaaccg ttagactggt
     8941 tgtggatcac cgcgtggcgg tggaagaagg taccgcgacc ggagtcttca cccgacaggc
     9001 gaaccggaat gccttcgtca accagcgtgg cgtaagcgag gttttccgca ccgccccagt
     9061 cgaacagttt ctcaccggca gccatcgcct ggcgatcgcc ataaatcttg gcaacgcgag
     9121 actgcatttc aactgcttcc ggcaccgtgc tgatgcgttt cgccagctcc tgcaggcgct
     9181 tcatctcaac tttgttcggg tactcttcgt cccattcgtg gttgaggtac ggcgaccagg
     9241 tgaaagagtg catgttcatc ggacgccact ctgccactac gcaatcgcca gcatccagcg
     9301 catcgcggta caggttaacc atctcggtgg catcttccag cgtcgccact ttttcctgct
     9361 ccagcttgtc agcgtagatt ttgcgcggtg tcggatgttt tttgattttc tgatacatca
     9421 gcggctgggt tgcgctcggc tcgtcggctt cgttgtggcc gtgacggcgg tagcacacca
     9481 ggtcgatgaa gacatcacgt ttaaaggtgt tacggaaatc gagcgccaga cgggtcacaa
     9541 aggcaacggc ttccggatcg tccgcgttaa cgtggaaaat cggggcctga accatcttac
     9601 cgatatcagt acagtacggc gtagaacggg catccagcgg attagaggtg gtgaaaccaa
     9661 cctggttgtt gataacgata cgtaccgtac cgccaacttc ataaccacgc gctttcgaca
     9721 tgttcagggt ttcctgaacc acgccctgcc cggtcactgc ggcgtcaccg tggatggtga
     9781 ttggcagcac tttgttgctg ctcggctcat caagtctgtc cagacgggca cgaacagaac
     9841 cgataactac cgggcttaca atctcaaggt gagacgggtt aaacgccagc gccaggtgca
     9901 ccaggccgcc atcggtctgg aagtcagacg agaagcccat gtggtatttc acgtcacccg
     9961 tgccgaggtg ttctttatgt ttaccggcga actcgtcgaa caagtcttgc ggttttttac
    10021 ccagcacgtt caccagcacg ttcagacgac cacggtgcgc catcccgaga accacttcgc
    10081 gggtgccgct gttgccagcg tggcggatca tctctttaag catcgggatt aacgcgtcac
    10141 cgccttccag cgagaagcgt tttgcgccag ggaattttgc gccgaggtaa cgttcaaggc
    10201 cttcagcggc ggtcagttcg cttaagaagc gttttttctc ttcgctattg aaagtcgcgc
    10261 gaccagactc gatacgctgt tggatccagc gtttttcttc ggtgctggtg atgtgcatat
    10321 actcggcacc aatcgggccg cagtaggttt gcttgagggc ttccagcagc tcgccgagtt
    10381 tcatggtttc tttgccgctg gcaaatgaac cgacgttgaa ggtttcctgg aagtctgctt
    10441 cggtcagatc gtggaaagac ggatccagat cggccacttt atcttgctgc cacagtccca
    10501 gcggatcgag attcgcatgc tggtgaccac ggaagcggta tgcgttaatg agctgcagga
    10561 ctttaacctg cttcacattg gtgtcagggt cggagatcgt tgaagagtaa cgtgaagcgt
    10621 ctttcgccag gcggcggaaa tattcacgcg tttgagagtg gaattgatcc ggtttgactc
    10681 ccgtaccagg taactgctgg aacgtcgaac gccagttagc gtcaaccgag tcaggatcgg
    10741 ttaagaagtc ttcatagagc tgttctatcc agctctggtt tgcgccagag aggtaagaag
    10801 agtccaacca ggctttcaaa gcgctgttct gcatcgtgat cccttaagca tcttttttat
    10861 gcttacttcg ccgtggatac taccacgcac agtgatgtgc gtgccggggt tcacttgcga
    10921 gctcttgtct acgtagtggc tcgcgaagga acctttaaaa actgtcttag tgtcaggggc
    10981 agtttttaaa gatttcctgc atttctttgt tacgcctgat gcgctgcgct tatcaggcct
    11041 acgggtaatg acttgtaggc cggataaggc gttcacgccg catccggcac tggttgcctg
    11101 atgcgacgct tgcgcgtctt atcaggccta cggtttacgc attacgttgc aacaacatcg
    11161 acttgatatg gccgatggcg cgcgtcgggt tcagcccctt cggacataca ctgacgcagt
    11221 tcatgatgct gtgacagcgg aatacgctga atgcatcgct caaaccgtcg aggcggctgt
    11281 cagtctcggt atcacggcta tcgatcagga aacgatatgc cgctaacaag cctgccgggc
    11341 cgataaactt atcgggattc caccagaaag acggacaaga ggttgaacaa catgcgcaga
    11401 gaatacattc atacagcccg tcgagttttt cgcgctgctc tggcatctgt aaatgctcgc
    11461 gagctggcgg attttgtcca ttattcaaca ggtaaggctt aattttctca tattgcgcat
    11521 agaattgtcc catgtctacc accaaatcgc ggatcaccgg taaacctggc agcgggcgaa
    11581 tcacaatctt cttgcccggc tggttgagtg ccgaaatcgg ggtaatacag gccagaccat
    11641 tcttgccgtt catgttcaga ccgtcggaac cgcacacacc ttcacgacag gagcggcgga
    11701 acgacaggct gggatctttc tctttcagct ggataagcgc atccagcagc atcatgtcgc
    11761 gaccttcttc cgcttccagg gtgtaatcct gcatacgcgg agcatcatca acatccgggt
    11821 tatagcgata aattgaaaac tcgagtctca ttttcctgtc tccgcattag taagtacgaa
    11881 tcttcggcgg gaatgccggg cgcagtttcg gttccatgtt gacgcttcgg cgcgtcatgg
    11941 attccgactc tggcagatac agggagtggc acagccagtt ttcatcatca cgatccggga
    12001 agtcgaagcg gctatgcgcg ccacggcttt cggtacggaa gttggcagaa acagccgttg
    12061 catacgccgt ttccatcagg ttatccagtt ccaggcactc aacgcgctgg gtgttgaact
    12121 cgctggaagt gtcatccaga cgggcatttt tcagacgctc gcggataact ttcaactgct
    12181 caagcccttt cgccatcgca tcaccttcac ggaagaccga gaagttatgc tgcatacatt
    12241 cttgcagcgc tttacgaatc gccaccggat cttcaccgtt acgattattg ttccagcggt
    12301 tcagacgatc cagagatgct tcaacatcag actcgctggc atcgcgcaat gcgccctgct
    12361 cggcgataga ctcttgcaga tgcagacctg ccgcacgacc aaagaccacc aggtccagca
    12421 gcgagttgcc gcccagacgg ttagcgccgt gtaccgatac acaagcgatt tcaccaacgg
    12481 caaacagtcc cggaacaacc acatcttcgc ctttctcatt cacagtcagc gcctgaccgg
    12541 tcactttggt cggaataccg cccatcatgt aatgacaggt tgggataacc gggatcggct
    12601 ctttcaccgg atcgacgtga gcgaaggtac gggagagttc caggatgccc ggcagacggg
    12661 attcgagaac ttctttaccc aggtggtcga gtttcagttt cgcgtgtggc ccccacggac
    12721 catcacagcc gcgaccttca cggatttcga tcatgatgga acgcgccacc acatcacggc
    12781 ccgccaggtc tttggcgttc ggcgcataac gctccataaa gcgttcgcca tgtttgttca
    12841 gcagataacc gccttcacca cggcaacctt cggtgaccag tacgcccgca ccggcaatac
    12901 cggtcggatg gaactgccac atttccatat cctgcaccgg tacgcctgcg cggatagcca
    12961 tgccgacgcc gtcgccagtg ttaatgtggg cgttagtggt ggactgataa atacgccctg
    13021 ccccgccagt cgccagcacg gtagcgcggg ctttgaaata aaccacttca ccagtttcga
    13081 tgcacagtgc agtacaaccc accaccgcgc catcctggtt cttcaccaga tccagcgcat
    13141 accactcgga gaaaatggtg gtgtggtttt tcaggttctg ctgataaagc gtgtgcaaca
    13201 gtgcgtgacc ggtacggtca gccgccgccg cagtgcgtgc cgcctgctcg ccgccgaagt
    13261 ttttcgactg accgccaaac ggacgttgat agatacgacc atcatcaaga cgcgagaacg
    13321 gcaggcccat gtgttcgagt tccagaatcg cttccggccc ggttttacac atatattcaa
    13381 tcgcatcctg gtcaccgata tagtccgacc cttttacggt gtcgtacata tgccattccc
    13441 agttatcttc atgggtatta cccagcgcaa cggtaatgcc gccttgcgca gaaacggtat
    13501 gggaacgggt cgggaagact ttagagagca gcgcacaggt ctggccgctc tgggaaattt
    13561 gcagcgccgc gcgcatacct gcgccaccgg caccaatcac aactgcatca aattctctga
    13621 ctggcaattt catcacacac cccacaccac aacgaatcca taaatcacgt aaaccaccag
    13681 tgcaacgaca atcaccagtt gcagcatcag gcgcaatgcc agcggtttaa cgtagtcggt
    13741 caacacctgc cacatgccga tccaggcatg aatcaagata gaaaacagcg ccagcagggt
    13801 gaacactttg gtgaacgcag aggcgaagaa accgatccag acttcatatg tcagttcgcc
    13861 actggtggca aaaaaaccga ccatataaat gatgtagagc gtcaggacga tagcggtagc
    13921 gcgaacgagg atgaaatcat gtacgccatt gcgtcctaat gcggaggcgt tgcttaccat
    13981 acgaggactc ctgcgagaag tgaaagcacg acagtaataa caaaggagat tttggcggag
    14041 cgtttacccg cttcgaatgt ttcttccaga tagccaaaat ccatcatcat gtggcgaata
    14101 cctacgacga cgtgatacgc cagagcggta aggatgcccc acatgataaa tttgacgaag
    14161 aagctgccca taatcgcgga agcttgctcg aaaccttcag gggaagagag gctggtaccc
    14221 agaagccaca gcaggatgcc cactgcaaca aaggtgatca caccggaaac gcgatggaga
    14281 atggacgcta tcgccgtgat ggggaaccgg atggtctgta ggtccagatt aacaggtctt
    14341 tgttttttca catttcttat catgaataac gcccacatgc tgttcttatt attccctggg
    14401 gactacgggc acagaggtta actttctgtt acctggagac gtcgggattt ccttcctccg
    14461 gtctgcttgc gggtcagaca gcgtcctttc tataactgcg cgtcatgcaa aacactgctt
    14521 ccagatgcga aaacgacacg ttacaacgct gggtggctcg ggattgcagg gtgttccgga
    14581 gacctggcgg cagtataggc tgttcacaaa atcattacaa ttaacctaca tatagtttgt
    14641 cgggttttat cctgaacagt gatccaggtc acgataacaa catttattta atttttaatc
    14701 atctaatttg acaatcactc aacaaagttg ttacaaacat taccaggaaa agcatataat
    14761 gcgtaaaagt tatgaagtcg gtatttcacc taagattaac ttatgtaaca gtgtggaagt
    14821 attgaccaat tcattcggga cagttattag tggtagacaa gtttaataat tcggattgct
    14881 aagtacttga ttcgccattt attcgtcatc aatggatcct ttacctgcaa gcgcccagag
    14941 ctctgtaccc aggttttccc ctctttcaca gagcggcgag ccaaataaca aacgggtaaa
    15001 gccaggttga tgtgcgaagg caaatttaag ttccggcagt cttacgcaat aaggcgctaa
    15061 ggagacctta aatggctgat acaaaagcaa aactcaccct caacggggac acagctgttg
    15121 aactggatgt gctgaaaggc acgctgggtc aagatgttat tgatatccgt actctcggtt
    15181 caaaaggtgt gttcaccttt gacccaggct tcacttcaac cgcatcctgt gaatctaaaa
    15241 ttacttttat tgatggtgat gaaggtattt tgctgcaccg cggtttcccg atcgatcagc
    15301 tggcgaccga ttctaactac ctggaagttt gttacatcct gctgaatggt gaaaaaccga
    15361 ctcaggaaca gtatgacgaa tttaaaacta cggtgacccg tcataccatg atccacgagc
    15421 agattacccg tctgttccac gctttccgtc gcgactcaca tccaatggca gtcatgtgtg
    15481 gtattaccgg cgcgctggcg gcgttctatc acgactcgct ggatgttaac aatcctcgtc
    15541 atcgtgaaat tgccgcgttc cgcctgctgt cgaaaatgcc gaccatggcc gcgatgtgtt
    15601 acaagtattc cattggtcag ccatttgttt atccgcgcaa cgatctctcc tatgccggta
    15661 acttcctgaa tatgatgttc tccacgccgt gcgaaccgta tgaagttaat ccgattctgg
    15721 aacgtgctat ggaccgtatt ctgatcctgc acgctgacca tgaacagaac gcctctacct
    15781 ccaccgtgcg taccgctggc tcttcgggtg cgaacccgtt tgcctgtatc gcagcaggta
    15841 ttgcttcact gtggggacct gcgcacggtg gtgctaacga agcggcgctg aaaatgctgg
    15901 aagaaattag ctccgttaaa cacattccgg aatttgttcg tcgtgcgaaa gataaaaatg
    15961 attctttccg cctgatgggc ttcggtcacc gcgtgtacaa aaattacgac ccgcgcgcca
    16021 ccgtaatgcg tgaaacctgc catgaagttc tgaaagagct gggcaccaaa gatgacctgc
    16081 tggaagtggc tatggagctg gaaaacatcg cgctgaacga cccgtacttt atcgagaaga
    16141 aactgtaccc gaacgtcgat ttctactctg gtatcatcct gaaagcgatg ggtattccgt
    16201 cttccatgtt caccgtcatt ttcgcaatgg cacgtaccgt tggctggatc gcccactgga
    16261 gcgaaatgca cagtgacggt atgaagattg cccgtccgcg tcagctgtat acaggatatg
    16321 aaaaacgcga ctttaaaagc gatatcaagc gttaatggtt gattgctaag ttgtaaatat
    16381 tttaacccgc cgttcaaatg gcgggttaat ttttatatgc ctaaacacaa aaaattgtaa
    16441 aaataaaatc cactatcgga cattcatata ttctaa
//
LOCUS       DAJTNA010000085        15906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTNA010000085 DAJTNA010000000
VERSION     DAJTNA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15906
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.086"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..173)
                     /locus_tag="OO161_002313"
     CDS             complement(<1..173)
                     /locus_tag="OO161_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HCR5463359.1"
                     /translation="MFDYQVSKHPYFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWL"
     gene            complement(206..427)
                     /locus_tag="OO161_002314"
     CDS             complement(206..427)
                     /locus_tag="OO161_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5463360.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            553..1122
                     /locus_tag="OO161_002315"
     CDS             553..1122
                     /locus_tag="OO161_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HCR5463361.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEVTIRDITRLPGGRIFV
                     EGGNRAFDCKIEDIEIIGKIISLTVKYVR"
     gene            1138..1341
                     /locus_tag="OO161_002316"
     CDS             1138..1341
                     /locus_tag="OO161_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463362.1"
                     /translation="MLGKVFFVVLSCSLSCSLLLSPLTTYAKNYPCSGKKGGVSHCTS
                     DGKFVCNDGTISKSKKICTKNSR"
     gene            1602..2582
                     /locus_tag="OO161_002317"
                     /pseudo
     CDS             1602..2582
                     /locus_tag="OO161_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2687..3223)
                     /gene="rcdA"
                     /locus_tag="OO161_002318"
     CDS             complement(2687..3223)
                     /gene="rcdA"
                     /locus_tag="OO161_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5463363.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            3307..4515
                     /locus_tag="OO161_002319"
     CDS             3307..4515
                     /locus_tag="OO161_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5463364.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPVIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGAWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            4515..5330
                     /gene="ybjI"
                     /locus_tag="OO161_002320"
     CDS             4515..5330
                     /gene="ybjI"
                     /locus_tag="OO161_002320"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5463365.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            5416..5700
                     /locus_tag="OO161_002321"
     CDS             5416..5700
                     /locus_tag="OO161_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463366.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPTPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(5741..6970)
                     /gene="mdfA"
                     /locus_tag="OO161_002322"
                     /pseudo
     CDS             complement(5741..6970)
                     /gene="mdfA"
                     /locus_tag="OO161_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            7259..7855
                     /gene="ybjG"
                     /locus_tag="OO161_002323"
     CDS             7259..7855
                     /gene="ybjG"
                     /locus_tag="OO161_002323"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5463367.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7913..8671
                     /gene="deoR"
                     /locus_tag="OO161_002324"
     CDS             7913..8671
                     /gene="deoR"
                     /locus_tag="OO161_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5463368.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(8718..9920)
                     /gene="dacC"
                     /locus_tag="OO161_002325"
                     /pseudo
     CDS             complement(8718..9920)
                     /gene="dacC"
                     /locus_tag="OO161_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            10167..10792
                     /gene="gstB"
                     /locus_tag="OO161_002326"
                     /pseudo
     CDS             10167..10792
                     /gene="gstB"
                     /locus_tag="OO161_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(10789..11904)
                     /gene="yliI"
                     /locus_tag="OO161_002327"
     CDS             complement(10789..11904)
                     /gene="yliI"
                     /locus_tag="OO161_002327"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR5463369.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKISEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(12015..12398)
                     /gene="bssR"
                     /locus_tag="OO161_002328"
     CDS             complement(12015..12398)
                     /gene="bssR"
                     /locus_tag="OO161_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5463370.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            12611..13936
                     /gene="rimO"
                     /locus_tag="OO161_002329"
     CDS             12611..13936
                     /gene="rimO"
                     /locus_tag="OO161_002329"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5463371.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVNPGDILRVKVEHADEYDLWGSRV"
     gene            complement(13983..15311)
                     /locus_tag="OO161_002330"
     CDS             complement(13983..15311)
                     /locus_tag="OO161_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5463372.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIHVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAVINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(15319..>15906)
                     /locus_tag="OO161_002331"
     CDS             complement(15319..>15906)
                     /locus_tag="OO161_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5463373.1"
                     /translation="GFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNE
                     PHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGT
                     GLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDV
                     ARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         4101 a         3932 c         3767 g         4106 t
ORIGIN      
        1 agccagattt ctggtgcggt taataaatga ggctgagctg ggttcagctt gttccgcaga
       61 atctgcacat tcatgcctgc acgttctgcc agttgcacca gattgtggcg cagtgcaaat
      121 gcacgacagg cttcatcaaa atatggatgt ttggaaactt ggtaatcaaa catggtcaat
      181 gcctctgatg tatttcagaa tcgaactaat taaggtttag attgcattct gaaagcgcat
      241 caacggtcat tgctgctatg ttgatcatca ctttttcgcg ttttttatct ttgcgcagac
      301 ggtgacggat aaggcgtcca tcagccaaca tgtcattgat ggtatcgatg gatagccctg
      361 tcagctcgct atagcgttca atagtcacat gaggcgtggt aagagtgatt gaaatgttag
      421 gtctcatgat gcaacattcc tcgtttaatg atgattaatc aggacgaata cggatcgttt
      481 gtattttgtg aacaccataa gcatacgatc gcacagtgaa atcgccaaga taaaagttca
      541 cttggagtga ccatgaattt ggagaaaggc ggacgaggcg ccatagagcg catggtagaa
      601 gcttatggat tcaagactcg acaggcgttg tgcgatcatt taggaatctc taaaagtaca
      661 ctcgccacac gctacatgcg tgactcattc ccagcagaat gggtaatcca gtgcgccctt
      721 gaaacgggca cctcgcttaa ttggctcaca accggacatg gttcaaagca aacttcaggt
      781 aatacaaata ctatggaagt tgctaaatat gtattatctg atggtgcctt gcgtgaagac
      841 ggtttttata tttttgataa gggatttcta ccctctacgt ttaaaaaacc ttttgtcatc
      901 acagataaca attctgaatt tatttgtgat aaagaatttg atgacatacg tgatggtaaa
      961 tgggtaataa gtattgatgg cgaagtaacg atccgtgaca ttactcgttt acccggtgga
     1021 agaatcttcg tcgagggtgg aaacagagcc ttcgactgta agatagaaga cattgaaata
     1081 attggtaaaa ttataagttt aacagttaaa tatgttagat aataccggga ggaaactatg
     1141 cttggtaaag tattttttgt ggttttatca tgctctttat catgctcttt gttattaagc
     1201 ccactaacta cctatgctaa aaattatcct tgttctggga aaaagggagg tgtctctcac
     1261 tgtacctccg atggaaaatt tgtttgcaat gatggaacta ttagtaaatc caaaaaaatc
     1321 tgtactaaaa actcacgata acttttgctt ttatatctgc gtctaaaata aaaatgagcc
     1381 gcaggttaac cgcaaaagtt acatgctcac atagcaaaaa gaatagccaa cttcattatg
     1441 gcttcagtga gatgtatggt cgtaggattt catacattga cactggttat acatacagta
     1501 aaaatactct ctactggagg gcatttttta tggcagtacg aaaactcacc actgggaaat
     1561 ggctttgcga atgttaccct gccggacgaa gtgggcgtcg tgtgcgtaaa caattcgcca
     1621 ccaaaggcga agcactggct tttgagcgcc acacgatgga agaaaccgaa gcaaagccct
     1681 ggctgggtga atcagtggat cgtcgaacac tgaaagacgt ggttgagcta tggttcaaac
     1741 tacatggtaa atctctgaca gctgggcagc atgtctatga caaattgctg ttgatggttg
     1801 acgctctggg caatcctctt gcaaccgatc tcacctctaa aatgtttgcc cactatcgag
     1861 ataaacgcct gacaggcgag atctacttca gcgagaaatg gaagaaagga gcaagcccgg
     1921 tcaccattaa cctggagcaa agctatctaa gtagtgtttt tagcgaacta tcccgtctgg
     1981 gcgaatggtc gtatccgaac ccactggaga acatgcgaaa attcaccatc gcagaaaaag
     2041 agatggcatg gcttacccat gagcagattg ttgaattgct ggctgattgc aaacgtcagg
     2101 acccaattct ggcactggta gttaagatat gcttaagcac aggcgcacgc tagcgtgaag
     2161 ccgtaaatct tacccgctca caggtgacca aataccgaat tacctttgtc agaacgaagg
     2221 ggaagaaaaa cagaagcatc cctatcagta aagagcttta cgaagagatc atggcgcttg
     2281 atgggttcaa tttcttcaca gactgctatt ttcaattttt atccgtgatg gaaaaaacgt
     2341 ctatcgtgct ccctcgcggt caactcacac acgttctgcg ccatacgttt gcggcgcact
     2401 tcatgatgtc gggtggaaac attctggcct tacaaaaaat tctcggacac cacgatataa
     2461 aaatgactat gcgttacgca catctggcac tggatcatct ggaaacggcg ctccgtttca
     2521 accctctggc aacgctgcca agtggcgaca aagtggcggc agcggttggc attaccccgt
     2581 aataaccacc actgaccacc aacctaactt attgttttta atctaactta ttgttttcat
     2641 taacccgttt acataaatgg tttttttgtt atctaaaatt tatctactac cctgcaaccc
     2701 tctcaaccat cctcaaaatc tcctcgcgcg atagcggctt acggtcggtg acaaaatgca
     2761 gcgtcatccc ctcaataaac gcatcaagcg cgcgggcggt tccgggttca aaccattgtt
     2821 cgagcgtttg ctgactgcgc tgcatccagt tttgcattac cgtttttaat agcggttttc
     2881 ggctagccag cgcgtagagc tggtacatca gctccatatt atccggcgtt gcaacctgtg
     2941 agctgtagat catatcggtg atagcctggc atgcgccttg agcatcacta acatcgctaa
     3001 aaaatgcctg atattgacgg gacataatct cagtaaaact gctgaacgcc tccagcaaca
     3061 actcatcaat tcctgaaaaa tagtaggtca tcgatcccaa cggtaccccg gcaagggtag
     3121 caattttgcg gtgcgtaaca gcatgtattc cgtaaagttt caccgcctcc agcgtggcct
     3181 ggataatttt ttctcgccgt tgcggatcgt tagcgcgacg catgtcattt cctctgtctg
     3241 caattgtgta caaatgtaca caaccttgct aatgttgtct tccctcttct tattttgacg
     3301 tgatctatga ccgtaaattc ttcacgtaat gcattgaaac gccgaacctg ggcgctgttt
     3361 atgttcttct ttttgcctgg cctgttaatg gcgtcctggg caacccgtac gcctgttatc
     3421 cgcgatattc tctctgtctc gatcgctgaa atgggcggtg ttctctttgg tctgtcgatc
     3481 ggttcgatga gcggtattct ctgctcggcg tggttagtga aacgctttgg gacacgtaat
     3541 gtcatcctgg tcacgatgtc ctgcgcattg atcgggatga tgatattaag tctggcactc
     3601 tggctgacat cgcccctgct ctttgccgtt ggtctcggcg tctttggggc aagttttggt
     3661 tctgcggaag tggcgataaa cgttgaaggt gccgccgttg agcgagaaat gaataaaacg
     3721 gttttgccga tgatgcacgg tttttatagc ctgggcacgc tggcaggcgc tggtgtcggg
     3781 atggcactga cggcctttgg cgttccggca acggtgcaca ttttattggc ggcgctggta
     3841 ggtatcgcgc ctatttatat cgccattcag gcaatccctg acggtacggg caaaaatgct
     3901 gccgatggca cccagcatgg cgaaaaaggc attccttttt atcgcgatat ccagttgctg
     3961 ttgattggtg ttgtagtgct ggcgatggcc tttgccgaag gttctgccaa cgactggtta
     4021 cccttattaa tggttgacgg tcacggtttt agccctactt ccggctcgct gatttatgcc
     4081 ggttttaccc tggggatgac cgttggacgc ttcactggcg cttggttcat cgaccgttac
     4141 agtcgcgttg ccgtggttcg ggccagtgca ctaatggggg cgttgggtat tgggctgatt
     4201 atttttgtcg atagcgcctg ggtcgctggg gtgtctgttg tactctgggg actgggtgcc
     4261 tcgctgggct tcccgctgac catttctgcc gccagcgata ccggccccga tgcaccaacc
     4321 cgcgtcagtg tggtagcaac gaccggttat ctggctttcc tcgtcgggcc gccgctgctg
     4381 ggctatctcg gcgaacatta tggattacgt agtgcaatgc tggttgtact ggcgctggtt
     4441 attctcgcgg ctattgtcgc gaaagccgtc gccaaacccg ataccaaaac gcagacggcg
     4501 atggagaata gttgatgagc attaaattaa ttgcggtaga catggatggt actttcttaa
     4561 gcgatcaaaa aacctataac cgtgagcggt ttatggctca gtatcagcaa atgaaagcac
     4621 aaggcattcg ctttgtggtc gccagcggga atcaatatta tcagttgatc tctttcttcc
     4681 ctgaaattgc taatgaaatt gcctttgtgg ctgaaaacgg cggctgggta gtgagtgaag
     4741 gcaaagatgt ttttaatggc gagctgtcga aggatgcctt tactactgtc gtggaacatt
     4801 tgctgacgcg cccggaagtg gaaattattg cctgcggaaa aaatagcgcc tatacgctca
     4861 aaaaatatga cgatgccatg aaaacggtgg cggaaatgta ttatcaccgt ctggaatacg
     4921 tcgataactt tgacaactta gaagatattt tctttaagtt tggcctgaat ctttccgatg
     4981 aactgattcc acaagtacaa aaagcattac atgaggccat cggcgatatt atggtgccag
     5041 tccacaccgg caacggcagc atcgatctga ttatccccgg cgtacataaa gccaatggcc
     5101 ttcgccaact gcagaaatta tggggaatag acgacagcga agtggtggtc tttggcgatg
     5161 gcggtaacga tattgagatg ctgcgtcagg caggctttag ttttgcaatg gaaaatgccg
     5221 gcagcgcggt cgtcgcagcg gcaaaatacc gggcaggctc caataaccgt gaaggcgtac
     5281 tggatgtgat cgataaagtg cttaaacacg aagcgccatt tgaccaataa aaacggcagc
     5341 acgcccggca acacaatacc tgaatgaagg ctgcataatt cgcggaaatt aacccgctgc
     5401 attatttcag gcgaaatgat tatgaaaaat tgtctactgt tgggcgcgct tttaatgggc
     5461 tttactggtg tggcgatggc gcaaagtgtc accgtcgatg tgccgagtgg atacaaagtg
     5521 gttgtagttc ctgattcagt cagcgttcca caagcggtca gcgtcgcgac tgtgccgcaa
     5581 accgtctatg tggctcctac gccagctccg gcttaccgcc cgcatcctta tgctcgccat
     5641 cttgccagcg taggtgaagg gatggttatc gaacaccaga ttgacgacca tcaccattaa
     5701 ttcgagaatg cctaatcgca caaagcagtc aggcattttt ttacccttcg tgagaatttc
     5761 ccatctgttt atcttttaaa aagataacca tcagcgacaa ccacaaaatt ccgttgacaa
     5821 ggttgaagag attaaactgt ccgttgcccc cgttcagcca ggcatgtttg ctgatttcaa
     5881 taccaacggt aaagatcagc atttgcagca ttcccatcgc cgcagaaacc gtacctttac
     5941 tcatatcgct ggcaaacagg gttaatcgca ccagtcccgc attcgccaga ccaataccga
     6001 aagcataaat acttaacccg gcagtcatcc ataaatacgc gtgcgatgag ataaccgttg
     6061 ccgcagcagc gaccaacaga ccaatcataa tcggccagcc gcccataatt atcagcgaac
     6121 gtacggtgcg gcgcgaggtc agacgcgcta acagcaagtt acccgcaatt aacgccccga
     6181 aaataggcac ttgcagcaag ccatattcat agctgctcaa ctgctcgccg gtaatgatga
     6241 taatcggcga ctgggcgatc cacgccagca atggcaggct aacgaatccc agcgccagcg
     6301 cccccgccac aaagcggccg ttcttcagca ccagcttata gtcacgaccg agttctttca
     6361 gtgacagttt ctcgcctata cgcgtggcgg tttcaggcat agctcgttgc agaccgaaaa
     6421 aggagatcgc tgccaatgcg gcaaacaaaa caaacatccc ctcccagggc agcacatgga
     6481 tccacgccgc gcccaccagc ggaccaagta gcggagcaat cagcgccacg ttcgccatca
     6541 gcgcggtgat cttgatacaa accgcctctt cgaaagattc ctgaattgcg gcgtatccca
     6601 cagcgccaat gaaacagagg cttatgccct gcaagaagcg caacagagtg aattgttcaa
     6661 tgttttgcgc cagtaatatt gccagacagg tgatgataaa ccacaccccc ccgccagcat
     6721 caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta aaaacatccc
     6781 gcccgccaga tatgcggtca ttgaagtagg aacccaatca atgcccgcct gatattgttc
     6841 caccacggcc aacataccgg gttgaatcat atctgccgat ataggttgaa aattcgtaaa
     6901 gcaccagaca gagagggaaa agtaacgcct gacgtccaag cctggcaccg gaagctaatt
     6961 tattttgcat gcaatttctt cgccaataat aatcgcgcag agtttaataa aagcgcagct
     7021 aacgagaaag cgaattttgt agctgaaacc acggttaagc acattcttac attattgcga
     7081 gtatagctac gctttcttta agttttattt aacctatgcc cgttacaatc acccaccgta
     7141 aacaggccgc ttgagggaaa taagacgatg ccgctttacc cacccagttt aacctgcact
     7201 ttattctcaa cgacttgcct gtattggctc ccttttaatc actttgcgtc gggaagttat
     7261 gctggaaaat ttgaatctct ctctattctc tcttattaac gcgacgccag actcggctcc
     7321 gtggatgatc tcgttggcga tttttattgc taaagatttg attaccgtgg tgccgctgct
     7381 ggccgcggtg ctttggttgt gggggcttac agcacaacgg caactggtga taaaaatcgc
     7441 tatcgcgctg gcggtcagcc tgtttgtgtc ctggacgatg ggacatcttt ttccgcacga
     7501 ccgacccttt gtcgaaaata tcggctataa cttcctgcat catgcggcgg atgactcatt
     7561 cccaagcgac cacggtacgg tgattttcac ctttgcactg gcatttttat gctggcatcg
     7621 cctgtggtcc ggctcacttt taatggtgct ggccgtcgtc attgcctggt cgcgcgttta
     7681 tcttggcgtc cactggccgc tggatatgct cggtggattg ctggcaggta tgattggctg
     7741 ccttagtgcc cagattatct ggcaagcgat ggggcataaa ctctatcaac gtctgcaatc
     7801 gtggtatcgc ttctgttttg cattaccgat ccgcaaaggc tgggtgcgtg actgatctct
     7861 gctaaaaagt gtagtattga gcggctcgct tcaataacta ttcagaggga ttatggaaac
     7921 acgtcgcgaa gagcgtatcg ggcagctgct gcaagaatta aaacgcagcg ataagttaca
     7981 tcttaaagac gccgccgccc tgcttggggt ttcggagatg acgattcgtc gcgatctgaa
     8041 caaccacagt gcgcccgtcg ttttgctcgg cggctatatt gttctggaac cgcatagtgc
     8101 cagccattac ctgttaagcg atcaaaaatc ccgcctggtg gaagaaaaac gccgggcggc
     8161 aaaactggct gcgacactgg tagaacccga tcagaccctc ttttttgact gtggcaccac
     8221 cacgccgtgg attattgaag cgattgataa tgaaatccct tttaccgccg tttgttattc
     8281 gctaaatacc tttctggcgc tgaaagagaa accccattgc cgcgcatttc tgtgcggtgg
     8341 tgaatttcac gccagcaacg ccattttcaa acccatcgat tttcagcaaa cgttgaataa
     8401 tttttgcccg gatatcgctt tttattccgc ggcgggcgtg catgtcagta aaggcgctac
     8461 ctgttttaat cttgaagagt tgccggtaaa acactgggcc atgtcgatgg cgcaaaagca
     8521 tgtgctggtt gtcgaccaca gtaaatttgg caaggtccgt ccggcgcgca tgggtgacct
     8581 gaaacgcttt gatattgtgg tgagtgattg ttgcccggaa gatgagtatg tgaagtacgc
     8641 gcagacgcac cgtattaagt tgatgtatta atgacgtata accggatgac gtttcgcgcc
     8701 atccggttat cagaagatta agagaaccag ctgccgaacc actgatggaa tttcatcatc
     8761 acgaaatccc acatccgacc aaagaatccg ccctcttcca cattttccat cacgatcagc
     8821 ggacgctgct caatggattt accgttaagc tggaaatcaa tggtcccgac aacctgacct
     8881 tttttcagcg gtgcggtaag ctgcggttcc gttaacgtgt agctcgcctt caggtttttc
     8941 agctgcccac gcggtatggt cactgagccc gcttcgcctg ccccgagatt cacttcgctc
     9001 ttatcaccaa accagacgcg ctgagtcaca aaggtggtat caggtttaat tggcgtcacg
     9061 gtttcaaaga agcggaaacc ctaggtcaat aatttctcag actcattaaa acggatacgg
     9121 tcagttttcg cccccaacac tacggagatt aaacgcatat cgccctgggt agccgaagca
     9181 accagattat atcccgcgcc tgccgtggtt cctgtcttca tgccatcaac attcagattg
     9241 ctgctccaca gcagacggtt acggttaggc tgacgaattt tgttgaaggt gaactctttc
     9301 tctttatgaa tggcgtactc ttccggcaca tcgtggatca atgctttacc cagcaatgcc
     9361 atatcgcgcg cggtactgaa ctgccccggc gcatccaggc cgtgcaccgt ctggaaggta
     9421 gtgttggtca gacccagttt tttggcataa ccattcatca gaccaataaa tgactcctgg
     9481 ctcccggcaa cgtaatcggc cagcgcaata caggcgtcat taccggactg gataatcaca
     9541 cctttgttca agtctgccac cgaaacctga tcgcccggtt tgaggaacat taccgatgaa
     9601 ccacgcagtg ccggatttcc cgtcgcccag gcatctttac cgaccgtcac catatcggtg
     9661 agtttaatct tatcggcctt aagcgcctgc ccaaccacat agctggtcat gattttagtc
     9721 aggctcgcgg gatccagttt ctcatccgca ttgccttctg ccagcacttt accgctggcg
     9781 taatccatta aaatccatgc acgcgcatcc acgctcggcg cttcaacggt ttgttcggcc
     9841 gcgaatgccg ttggggcaaa aaggaataaa aatgcagaac ccgctgcaag accacgaagg
     9901 agagaggagt attgcgtcat aaatgccacc cgagtatcca ttcaaaaaaa atacgttata
     9961 tcgccgtatg ataagttcgg tgattaataa cgcacacaca aacttaaaga aaccgtaagt
    10021 tggtaaagtt tttaaagttt atgcaatatc ctgccgcttc gctgcttctg gtgtcatttt
    10081 tttgccagct gttgcttaaa agttaacttt acttcaccat cggagtcctg ttcagcctgt
    10141 ggctgcctgt cggaaaaggg gtgagtatga ttacgctgtg gggtcggaat aattcaacca
    10201 acgtaaaaaa gtattgctga cgctcgaaga actggaacta ccttatgagc aaattctcgc
    10261 gggccgtgag tttgggataa atcacgatgc tgattttctg gcgatgaacc ctaacgggct
    10321 ggtgccgttg ttgcgtaacg acgaaagtga tctcattctt tgggaatcaa acgccattgt
    10381 ccgctatctg gcggcacagt acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg
    10441 tgcggaagcg gaaaaatgga tggactgggc aaaccagacg ctcagtaatg cccatcgagg
    10501 gatcctgatg ggattagtca gaacaccacc ggaagagcgc gatcaggccg ccattgatgc
    10561 cagttgcaaa gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg caaaagtaaa
    10621 atggttctct ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa
    10681 tttgtttaac gttggcctga cctggacacc gcgtccaaat ctgcaacgct ggtatcagca
    10741 actcactgaa cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg
    10801 gctaacttta agtaattccc cactggactc gtcggtgaga acgtataaat aaccgtcggg
    10861 tccagtgcga acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc catcttctgt
    10921 cactttgtcg ccgttgacgc tcatcacaat gacatcttta tctttcagcg cgccaataaa
    10981 taatttttgc tgccactggg ggaatttatc gctgttatag aaggccatgc cgctcacagc
    11041 gggcgaatct ttccagtaaa aaacaggttg ctcggtcccg gcgacgatct cccctttcgc
    11101 ttccgatatc ttaaagcctg aatagttgat tccccaggtt gccagcggcc agccgtagtt
    11161 tttgcctttt tgcgggatat taatttcatc accaccgcgc gggccatgtt cattcagcca
    11221 cagtgcatta ctccacggat tcatcgccat tccttgcgga ttacgaatgc cataagacca
    11281 gatctcggcg cgcgcaccgg attcctttat aaaaggatta tcatccggga tttcgccctg
    11341 atcggtcaga cgcaccagtt tgccctgtaa tttatccaga tcctgcgccg tcgggcgctg
    11401 attgttttcg cccagagcaa taaaaagata acctttaccg tcgaatacca gccgcccgcc
    11461 aaaatggttg ccggtagaca gttttggcat ctggcggaag acggtgcgga agtcggtcac
    11521 ttttgagaga tcatcactta agcggccata acccacagca gttccggctt tgccatcatc
    11581 gccaacttcg gaataactta accagatgcg gcgagactga gcaaaatcag gcgctaaaac
    11641 cacgtccagc aggccgccct gcccgtgcgc ccaaacgtcc ggaactccgg aaagcggcgc
    11701 agataatcct tttcctgctt gccagtgacg caactcgccg ccgcgcagag tgattaacat
    11761 accgtgatta tcgggtaaaa aggccagtgc ccagggatgg tcgagtttgt cttgcagtac
    11821 ttcgacattt accgttgcag gagccgccca gagagcggaa gaaagacaaa taaggggcac
    11881 aaggaaaaag gattgtcgat gcataacgcc tccggattga ctctggctta aagcgtagtc
    11941 agtggaggag ataacaaatt catttttaca aaaacttaaa catgaagggg gagacgcttt
    12001 ctccccctta gttttcaggc cttctcaagc atggcgtgct tctgcaggct ctggatactc
    12061 agcgttaagc tcatcagaca attttcaagc ttatcggcgt tgacggtaat aacagtcggg
    12121 caatcatggt gcccactcat caaacatact gcggctgtcg ctaatgcttc ttcagcatga
    12181 tgaagagcac tccactcttc ctgatccaga tgaagattca accgcagcga tttatcgtgc
    12241 agttcgcgat tcagtttaaa aaagttatct cgtagatgat tgctttcgct gacggacatg
    12301 tatccttttg ccttcctcag ttgcacgtat gcagcgaggt caacgcgtgc gtcgatcaac
    12361 cggttcagca gatcgattcg ctgtctgtca acaaacatac ttcgatcctc ctctttccag
    12421 ccttgttgca attaaatagt atggctattt tttgagcaaa atgctgtcca ggatcaattc
    12481 ttcacgccga attaacaaaa tttacatttc cttcgcctcc gccagcgaat gagattgcga
    12541 tacaaatttt atccagaact ctgtagaatc cctgccctga cctttcctgt aactgaatat
    12601 tttttaagct atgagcaaag taactcccca gccgaaaatc ggctttgttt cccttggctg
    12661 tccgaaaaac cttgtcgatt cagagcgtat tctcaccgaa ctccgcactg aaggttatga
    12721 cgtggtaccg agctatgacg atgcggacat ggtgatcgtc aacacctgcg gctttattga
    12781 cagcgcggta caagaatcac tggaagccat tggtgaagcg ttgaatgaaa acggcaaggt
    12841 aattgtgacc ggttgtctgg gggcaaaaga agatcagatc cgcgaagtcc acccgaaagt
    12901 gctggaaatc accgggcctc atagctatga gcaggttctg gagcacgttc atcactacgt
    12961 gccaaaaccg aaacacaacc cattcctgag cctggtgcca gaacaaggtg tgaaactgac
    13021 gccgcgtcat tatgcctatc tgaaaatttc ggaaggctgt aatcaccgct gcaccttctg
    13081 cattattccg tctatgcgcg gcgacctggt gagccgtccg attggcgaag tgttaagtga
    13141 agcgaaacgt ctggtagatg cgggcgttaa agagattctg gtgatctcgc aggatacttc
    13201 cgcctatggc gttgatgtta aacatcgtac tggcttccac aacggcgagc cggtaaaaac
    13261 cagcatggtc agcctgtgcg aacagttatc gaaactgggg atctggacac gtctgcacta
    13321 cgtttaccct tatccgcatg tggacgacgt catcccactg atggcagaag gcaaaatcct
    13381 gccgtatctg gacattccgt tgcagcacgc cagcccgcgc attctcaaac tgatgaagcg
    13441 tccgggttct gtagatcgcc aactggcgcg catcaaacag tggcgcgaaa tctgcccgga
    13501 actgacccta cgctcaacct ttattgtcgg cttccctggc gagacagaag aagatttcca
    13561 gatgctactc gacttcctga aagaagcgcg tctggatcgc gttggctgct ttaaatacag
    13621 cccggttgaa ggtgcagacg ccaatgccct gcctgaccag gttccggaag aagttaaaga
    13681 agaacgctgg aaccgtttca tgcagctgca gcagcagatt tccgccgagc gcctgcaaga
    13741 gaaagtgggc cgtgaaattc tggtaattat cgacgaagtg gacgaagaag gcgcgattgg
    13801 tcgcagcatg gcagatgcac cggaaatcga cggcgcggtt tatctcaatg gtgaaaccaa
    13861 cgttaatccg ggtgatatcc tgcgtgtgaa agtcgagcac gccgatgagt acgatttgtg
    13921 gggtagccgg gtttaattaa ttcaggccgg gcaaaatcat ttgtccggcc tgcgaataat
    13981 gattactgcc agctaatctg agccctgccg tttttcttac tcttatacag ccgctcatcc
    14041 gccagcattt tcaagcgttc gacggtggta cattcatttg aatgcgctac accgatactg
    14101 gtacttaaat taatgcttcc cacctggaaa tgaacgattt cttttagaat cgatagcaac
    14161 tcttccagcg ttccacccgt ataaatcacc gcaaattcgt caccgccgaa gcggtagatt
    14221 ttacctttat ctaccgggat ctgtgattta aggattttca ccactttcat caacacttca
    14281 tcgcccagca aatgaccgaa ggtgtcgtta atgtttttga atttatcgac gtcaaacagg
    14341 cacaaataac cgcctttttc cggcagcgct tttaaatctt cgtcaaagga ttttctgccc
    14401 attgctttgg tcaggtcgtc caggaacgcc gagttataca tactgtagag ttttttgaca
    14461 tcggcagccg aaaagaaaag cccgccatcc agtacgcgtt caaaatggtt attaatgacg
    14521 gcttctttct ccacaatacc acgattaatc actctgcgta ccagatagag gcaactggca
    14581 cccgatatca cagccaccaa tgcggcaaag aaaataatca atgtacagac ccgcaaataa
    14641 acatcttttt tatacgcaaa tacgtacaat ccggtcggca aataggcgaa gtgctccatc
    14701 gcttccgaaa cggtgaagat ggcgttttta gcattatatt catcaccggt caccggttta
    14761 ccgttcttaa atagagagaa catcaggttc tcgctgtcca tcgccagcga ttttgacagt
    14821 aaagaaaaac gctccagacc aagcaaaaat atcagactac gattgtgagt taccggatag
    14881 aggtaataga cgaaggtttt accgtcaaaa gagataaacc ctgtattttt gacatgtcga
    14941 tacttcacaa cctgcataat atctgcacaa taatttttct cgaactcctt tacatgaata
    15001 cattcgtcac tggaaatcag ataaggaccg acatcgtaca aataagaggg ggaaaccgag
    15061 tcggcgtaag ctgtgcgcaa accaaccaac agctgttcct ccgtgggatt tagcgtgttg
    15121 ataagatcaa ttgccacgct acgccgatat tcagcaatat ttgccaccgc aaaagcagta
    15181 tcaataacgg actgtttcag gctatacacc cgttccttta ccatttgagt gtagatcccg
    15241 caggcaactg ctgaaatcat gataaaaatc agcagactga ctgtaagtac gaacttattg
    15301 attctggaca tacgtaaact actcttttac taattttcca cttttatccc aggcggagaa
    15361 tggcataggt ttctggtaca gatacccctg agcctgaaca catcctaatt taattaattc
    15421 gcgcgcaacg tcttcgctct ctacgccttc cgcaatcacc gggatccggt tatagcgtgc
    15481 cagatcgaaa ataatgcgca caatttttcc cgcagtaccg aaaatatcgc cggtaaattg
    15541 cgagtccacc tttatgctgt ctggattaat ttcataaaaa cgtttcaggt ttgacaaacc
    15601 ggtgccaaag tcatcaatcg atatccgtac cccttgttta cgcagttgtt caatattgag
    15661 ataaaaatgt tcattaattc gctcgatcac ggtttcagtg atctcaacac agaggctgtt
    15721 cgccttcagg ccattttctt tcatgatggt ggtaaaacgc tcatgaaagt gcggttcgtt
    15781 taactgccag ggcgaaacat taatatgcag taagaaatca tccttcagcg acgcacgctg
    15841 actaaaatgt ctgaattcac ccattgccgt tttaataatc tgataaccca gctcattgat
    15901 aaaccc
//
LOCUS       DAJTNA010000086        15360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTNA010000086 DAJTNA010000000
VERSION     DAJTNA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15360
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.087"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            160..603
                     /locus_tag="OO161_002332"
     CDS             160..603
                     /locus_tag="OO161_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5463374.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRITLPT
                     TISRLNLTLTDVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            839..1657
                     /gene="ybhA"
                     /locus_tag="OO161_002333"
     CDS             839..1657
                     /gene="ybhA"
                     /locus_tag="OO161_002333"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5463375.1"
                     /translation="MTTRVIALDFDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1658..2716)
                     /gene="modC"
                     /locus_tag="OO161_002334"
     CDS             complement(1658..2716)
                     /gene="modC"
                     /locus_tag="OO161_002334"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5463376.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSIMKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQEEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2719..3408)
                     /gene="modB"
                     /locus_tag="OO161_002335"
     CDS             complement(2719..3408)
                     /gene="modB"
                     /locus_tag="OO161_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5463377.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3408..4181)
                     /gene="modA"
                     /locus_tag="OO161_002336"
     CDS             complement(3408..4181)
                     /gene="modA"
                     /locus_tag="OO161_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5463378.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGHPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4347..4496)
                     /gene="acrZ"
                     /locus_tag="OO161_002337"
     CDS             complement(4347..4496)
                     /gene="acrZ"
                     /locus_tag="OO161_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5463379.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4625..5413
                     /gene="modE"
                     /locus_tag="OO161_002338"
     CDS             4625..5413
                     /gene="modE"
                     /locus_tag="OO161_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5463380.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5481..6953
                     /gene="modF"
                     /locus_tag="OO161_002339"
     CDS             5481..6953
                     /gene="modF"
                     /locus_tag="OO161_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5463381.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLVSLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7214..8230
                     /gene="galE"
                     /locus_tag="OO161_002340"
     CDS             7214..8230
                     /gene="galE"
                     /locus_tag="OO161_002340"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5463382.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8240..9286
                     /gene="galT"
                     /locus_tag="OO161_002341"
     CDS             8240..9286
                     /gene="galT"
                     /locus_tag="OO161_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5463383.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDQDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9290..10438
                     /gene="galK"
                     /locus_tag="OO161_002342"
     CDS             9290..10438
                     /gene="galK"
                     /locus_tag="OO161_002342"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5463384.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10432..11472
                     /gene="galM"
                     /locus_tag="OO161_002343"
     CDS             10432..11472
                     /gene="galM"
                     /locus_tag="OO161_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5463385.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11674..12426
                     /gene="gpmA"
                     /locus_tag="OO161_002344"
     CDS             11674..12426
                     /gene="gpmA"
                     /locus_tag="OO161_002344"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5463386.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12584..13636)
                     /gene="aroG"
                     /locus_tag="OO161_002345"
     CDS             complement(12584..13636)
                     /gene="aroG"
                     /locus_tag="OO161_002345"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5463387.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13952..14332
                     /locus_tag="OO161_002346"
     CDS             13952..14332
                     /locus_tag="OO161_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5463388.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            complement(14458..>15360)
                     /locus_tag="OO161_002347"
     CDS             complement(14458..>15360)
                     /locus_tag="OO161_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HCR5463389.1"
                     /translation="RSLLSGWVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLP
                     GNKKTKTGRLWTYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYA
                     GFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTA
                     EQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWA
                     EADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVL
                     DVIADWPINRVGELLPWRVALPTE"
BASE COUNT         3849 a         4044 c         3900 g         3567 t
ORIGIN      
        1 atttttaaaa cagctatagc ttctggtctt gccgaagaag atccagcttc tgcaaccagg
       61 ccattcagaa ctgaagtgaa gcgcagtcga ctgttaattg acgagtatct gttaatcagg
      121 aaaatagcag acgtccagaa cgaatggttc ggcctttgca tggatcttgc actggtaaca
      181 ggacagcgcg aaggagatct ggcggctatg agatgggagg atataagaga tggcaggcta
      241 tacgtggaac aacaaaaaac aggggcaaaa attcgcatta ctctccccac cacgatttcc
      301 agattgaatt taacacttac cgatgtactg gataacttga aaaaaataaa cggtaaaaac
      361 gaaaagttac tcggagggaa gacagccagg acaatcgcag cacagttcag gattgcccga
      421 gacacatccg gattgaaatg ggaaggtgac ccaccgccat ttcatgaaat ccgaagttta
      481 tccggtcgcc tgcactcagc agaaaaaggc agtgatttca ctcaggcgct ccttggacat
      541 cgttcatcca gcatgacgga taaataccgt gacggacgag ggagggaatg gaaagacatt
      601 tagaattaat ttcgaatgat ttcgaaacac tacttgtaat taattgatta taaaggatta
      661 attcctaaga ggcatttgct tcatgaatgc tcctttgcat ttagcaactg actgtaacag
      721 ctaaaattag tcgcttttgg cggtgaatgc tgaattaaaa cgcagaattt tgtccgatag
      781 tggtatcggt gtaccattcg cgcagacgaa aattcttaac cttaatctgg aatacgccat
      841 gaccacacgc gtgattgctc tcgactttga cggcacctta ttgaccccga aaaagaccct
      901 gcttccttca tcgatagaag ccctggcccg cgctcgcgaa gcaggctatc aattaatcat
      961 cgtcacaggt cgccatcacg tcgctattca tcctttttat caggcgctgg cgctggatac
     1021 acctgctatt tgctgtaatg gcacctattt gtatgattat catgcaaaaa ccgtgctgga
     1081 agcggaccca atgcccgtta ataaagccct gcaactcatt gagatgctga atgaacacca
     1141 cattcacggt ctgatgtatg tggatgatgc aatggtctat gagcacccga ccgggcatgt
     1201 cattcgcacg tctaactggg cgcaaaccct gccgccggaa cagcgtccga ctttcacaca
     1261 agtcgcttct ctggctgaaa cggcgcaaca agttaacgcc gtatggaagt tcgccctgac
     1321 gcacgatgac atgccgcaat tgcagcattt tggtaagcat gtcgaacatg aactgggact
     1381 ggagtgtgaa tggtcctggc acgatcaggt tgatattgca cgcggcggca acagcaaagg
     1441 taaacgtttg acgaaatggg ttgaggcgca aggctggtcg atggaaaacg tcgtggcgtt
     1501 cggcgataac tttaatgata tcagtatgct ggaggccgct ggtacaggcg tggcgatggg
     1561 caacgccgat gacgcggtaa aagcgcgcgc caacattgtg attggtgata acaccaccga
     1621 cagcattgcc cagttcattt atagccacct gatttaatca ggcggttatc gacacacttt
     1681 taatttgcgc gtacagccac aggcctggtt tgatcgccag ttcatccctg gcccacgggc
     1741 tgatacgcgc ccacagcgtt ttaccgccga cttccagctc cacttcctcc tgaccgttgt
     1801 cgtcataact attaacaact tttgcccgca atacgttacg aatgctggtt tgctgcggcg
     1861 gttgtaaaac cagagaaaca tcggaagcct gaatgcggat acgtagcgca gcttgcagcg
     1921 gttcgtccag cttattgacc cacaaatgct gatcgcccag cgccagcgcg gtcatcgcgt
     1981 aatgcggatg atgctcaagc accgtcactt tcataatgct actttgttgc tctttcggca
     2041 gccacggatt catcacgcta ctaccccaga cttcttccag tgcgccaaag gctttcacct
     2101 gaccgttttc cagtaccatc actctgtctg ccagatggag gatctcatcc agcgaatggc
     2161 tgacatacaa catcgggatg ttgatttccc gcgtcagacg ttgcagataa ggcaacagtt
     2221 cgcgtttacg cggaatatcc agtgacgcca gcggttcatc cagcaacagc aattctggtg
     2281 ccgtcagcaa agcccgccca atcgctacgc gctgtttttc gcctccggac aggctgcctg
     2341 gtaaacggtc aagcaacggt tcaatgccta aaagcgccac cagcttatcg aactgatcga
     2401 ccatgctttt cgccatgccg tagcgcagat tgccacgcac tttgtaatgc ggaaacagcc
     2461 gcgcatcctg aaaaacatag ccaacgcgac gcttttctgg cgtcaggcag ataccttttt
     2521 cggcatcatt tagtacccga ccattgagga caatcctccc tttttgcggg cgcgtcagtc
     2581 cactgatggc gttaatcagc gaagtttttc cggcaccgga gacgccaaag atagcagtga
     2641 tgccattggc gggcagcgtt tcattaatag tcaggcaatg gttgcccaac gtctgggaaa
     2701 aattcagttc cagcatgatt agcgccccgc ccgttcacgg ctgattctgg ccagccattc
     2761 tgaaatcaac agggagatca tcgccagcgc aatagaaata atgcacagtc tcgccgcgcc
     2821 actttccccg ccgggggtct ggatcagggt atacatggca gagggaatgg ttcgcgtttc
     2881 gccaggaatg ttcgacacaa aggtgatggt tgcaccaaac tcaccgagag aacgagcaaa
     2941 agccagtacc gtaccaacaa taattcccgg taaggtcagc ggtaacgtga tagtaaagaa
     3001 aacgcgccag cgcccggcac ctagtgttct tgcggcctgt tcgagtttga catcaacccc
     3061 ttccagcgcc agacgaattg cccgcaccat cagcggaaac gacatgacgg cggcagcgag
     3121 aaccgcgccg cgccagctaa aggcgaaggt aataccaaac cagtcataca gacgttcacc
     3181 gataaatccg cgccgtccca tcgaaactaa taataagtaa ccgacgacca cgggcggtaa
     3241 caccagcggt agatgcagta cgctgtcgag cagagctttg cccggaaacg tgcaacgcac
     3301 cagtaaccag gcaaaaaaga tcccaaacgg caggctaaac agcacagcca gggaagaaac
     3361 tttcaggctt aataaaactg cctgccattc tggatcggtc agtatcatta cttggttgta
     3421 aatccgtaac gtttaaagat ttccgctgcc tgcggtccct tcagataatc ataaaatgct
     3481 ttcactgtcg cattgttatg cccttccaca acagcaaccg gatattccac ttttttatgt
     3541 gaatcttccg ggaaggtggc aaccactttt acccctttgc tgacaactgc gtcagaaccg
     3601 taaacaatgc ccaacggcgc ttcattgcgt tcgaccaacg ccagcgcgcc acgaacatct
     3661 tccgccggag ccaatttcgg ggagagcgta tcccatgcgc ccagtttttg cagtgcttct
     3721 tttgcataaa tgccagcagg gacatgttcc ggatggccaa ccgcgaggcg accgccattc
     3781 agcagcgaag tccagttagt tgtgctgtcg atgatgaaat ctttctgctc gctggctttc
     3841 ggtgctacaa cgaccaggct attgccgagc agtgtctggc gcgaagccgt atcgatcgct
     3901 tttttatcaa ccgcataatc catccatttc tgatcggcag agataaacag atccgcaggc
     3961 gctcccgctt caatctgacg ggccagagta gatgacgaag cgaaagaaga aaccacatcc
     4021 acgccttttt cttttttata ctgcgtagca atgtcctgca ttgcgttagt cagtgatgcg
     4081 gcggcgaaca ccgtgatttt cccttcatct gccagtgtat tgccagcaac agcgaaagag
     4141 agtgccgccc cggcaaacaa gttcaaccat ttacgagcca ttggtaaccc cttaatgtaa
     4201 cgttatatag gtgacaatat aacggctagc tcagaatttt cccagcagtt attgctaacc
     4261 tttgtgaggt agataagaaa aaatatcggc tggaaaagca ggaagttgag aaaaagaaat
     4321 gcccgactaa gtgggcattc agggaatcaa tgattttgtc cgggctggtc ttttttacca
     4381 acaccagaaa agatgttgaa tacttcacca agaccgtaaa tcagacccag gatgatggcc
     4441 atcacgacag gtaccatgat tacggcgaat accagacttt ttaataactc taacatggtc
     4501 aactccagat atagtcatga gactattcta accgctaagc acagaaaagc actccccttt
     4561 tgtgcggtca gctttgcgtg gcgttcgttt tccgtcacaa taagactttt gccaggacat
     4621 tgttatgcag gccgaaatcc ttctcaccct taagctccaa caaaaattat tcgccgaccc
     4681 gcgccggatt tcgctactaa aacacattgc gctttccggt tccattagcc agggagcgaa
     4741 agatgccggt atcagctata aaagcgcctg ggatgccatt aacgagatga atcagttaag
     4801 cgagcatatt ctggttgagc gcgcaacagg cggtaaaggt ggcggcggcg cagtactgac
     4861 ccgctatggt cagcgactga ttcagctcta tgacttactg gcgcaaatcc agcaaaaagc
     4921 ctttgatgtg ttaagtgacg atgacgccct gccgctgaac agcctgctgg ccgcgatctc
     4981 acgtttttca ctgcaaacca gcgcccgtaa ccagtggttc ggtaccatca ccgcccgcga
     5041 tcatgatgac gttcaacagc atgttgatgt cttactggct gacggaaaaa cacgcctgaa
     5101 agtcgcaatt accgcacaaa gcggcgcacg tctggggctg gatgaaggca aagaagtgtt
     5161 gatattgcta aaagcgccgt gggtaggcat tactcaggac gaagcggtcg cacaaaacgc
     5221 caacaaccaa ttaccgggta ttattagtca tattgagcgc ggcgcagagc agtgcgaagt
     5281 attaatggcg ctacccgacg ggcaaacact gtgcgccaca gtgccggtaa atgaagcgac
     5341 ttctcttcag caagggcaga atgtcacggc ctactttaat gctgacagcg tgattatcgc
     5401 cacgctgtgc taagcgtgtt gacaatttgt tatgaaacac gtatccctgt cagtaatcgc
     5461 tgcacaaaat gggatataaa atgtcatcgt tgcaaatttt gcaaggcacg tttcgtctta
     5521 gcgacacaaa aacgctgcaa ttgcctcagc taacgttaaa cgcgggtgat agttgggcgt
     5581 ttgtcggttc gaatggaagc gggaaatcgg ccctggcccg cgcgctggcg ggggaacttc
     5641 cgcttttgaa aggtgaacgg caaagccagt tttcccacat cactcgtctc tccttcgagc
     5701 aattgcaaaa actcgtcagc gacgaatggc agcggaataa caccgatatg ctcggccctg
     5761 gcgaagatga caccggacgc actacggctg agatcattca ggatgaagta aaggatgcac
     5821 cgcgttgcat gcaactggcg cagcagttcg gtattaccgc cctcctcgac cgacgcttta
     5881 aatacctttc cactggcgag acgcgaaaaa ccctgctgtg tcaggcgctg atgtcggagc
     5941 ctgacttgtt gattcttgat gagccgttcg atggcctgga tgttgcctca cgtcagcagc
     6001 tggctgagcg actcgtctcg ttacatcagt ccggtattac tctggtactg gtgctcaatc
     6061 gcttcgatga gatcccggag tttgtccagt ttgctggcgt gctggcggat tgcacgttag
     6121 cggaaactgg cgctaaagag gaactgctcc aacaagcact cgtcgcgcaa ctggcacata
     6181 gtgaacagct tgaaggtgtg caactgccgg agccggatga accttcagca cgtcacgcct
     6241 tacccgccaa cgaaccgcgc attgtgctga acaatggcgt ggtttcttat aacgatcgcc
     6301 ccattcttaa taaccttagc tggcaggtga atccaggtga acactggcaa attgtcgggc
     6361 caaatggtgc aggaaaatcg acgttattaa gcctggttac tggcgatcat ccgcaaggtt
     6421 acagcaacga tttgacgctt ttcggacgac gtcgcggcag cggcgaaacc atctgggata
     6481 tcaaaaagca tatcggttac gtcagcagta gtttgcatct ggattaccgg gtcagcacta
     6541 ccgtgcgtaa tgtgattctt tctggctatt ttgattcgat tggcatttat caggccgttt
     6601 cggatcgcca gcaaaaactg gtgcagcagt ggctggatat tctcggcatt gataaacgta
     6661 cggctgacgc tccgttccat agtctttcct ggggacagca gcatctggcg ctgatcgtcc
     6721 gcgcactggt gaaacatccg acgttgctta ttctcgatga accactacag gggcttgatc
     6781 cgctcaatcg ccagcttatc cgccgttttg ttgatgtgct gattagcgaa ggtgaaacgc
     6841 aattgttgtt tgtttcgcac cacgctgaag atgcgcctgc ctgtattacc catcgccttg
     6901 agttcgtgcc ggacggtgga ctctatcgct atgtgctgac aaaaatatat tgagtcggta
     6961 gtgctgacct tgccggaggc ggcctaagcg ccctctccgg ccaacggttc gacgcatgca
     7021 ggcatgaaac cgcttctttt ttcagataaa aagcgcaatc attcataaac cctctgtttt
     7081 ataatcactt aatcgcgcat aaaaaacggc taaattcttg tgtaaacgat tccactaatt
     7141 tattccatgt cacacttttc gcatctttgt tatgctatgg ttatttcata ccataagcct
     7201 aatggagcga attatgagag ttctggttac cggtggtagc ggttacattg gaagtcatac
     7261 ctgcgtgcaa ttactgcaaa acggtcatga tgtcatcatt cttgataacc tctgtaacag
     7321 taagcgcagc gtactgcctg ttatcgagcg tttaggcggc aaacatccaa cgtttgttga
     7381 aggcgatatt cgtaacgaag cgttgatgac cgagatcctg cacgatcacg ctatcgacac
     7441 cgtgatccac ttcgccgggc tgaaagccgt tggcgaatcg gtacaaaaac cgctggaata
     7501 ttacgacaac aatgtcaacg gtactctgcg cctgattagc gccatgcgcg ccgctaacgt
     7561 caaaaacttt atttttagct cctccgccac cgtttatggc gatcagccca aaattccata
     7621 cgttgaaagc ttcccgaccg gcacaccgca aagcccttac ggcaaaagca agctgatggt
     7681 ggaacagatc ctcaccgatc tgcaaaaagc ccagccggac tggagcattg ccctgctgcg
     7741 ctacttcaac ccggttggcg cgcatccgtc gggcgatatg ggcgaagatc cgcaaggcat
     7801 tccgaataac ctgatgccat acatcgccca ggttgctgta ggccgtcgcg actcgctggc
     7861 gatttttggt aacgattatc cgaccgaaga tggtactggc gtacgcgatt acatccacgt
     7921 aatggatctg gcggacggtc acgtcgtggc gatggaaaaa ctggcgaaca agccaggcgt
     7981 acacatctac aacctcggcg ctggcatagg cagcagcgtg ctggacgtgg ttaatgcctt
     8041 cagcaaagcc tgcggcaaac cggttaacta tcattttgca ccgcgtcgcg agggcgacct
     8101 tccggcctac tgggcggacg ccagcaaagc cgaccgtgaa ctgaactggc gcgtaacgcg
     8161 cacactcgat gaaatggcgc aggacacctg gcactggcag tcacgccatc cacagggata
     8221 tcccgattaa ggaacgacca tgacgcaatt taatcccgtt gatcatccac atcgccgcta
     8281 caacccgctc accgggcaat ggattctggt ttcaccgcac cgcgctaagc gcccctggca
     8341 gggggcgcag gaaacgccag ccaaacaggt gttacctgcg cacgatcaag attgcttcct
     8401 ctgcgcaggt aatgtgcggg tgacaggcga taaaaaccac gattacaccg ggacttacgt
     8461 tttcactaat gactttgcgg ccttgatgtc tgacacgcca gatgcgccag aaagcaacga
     8521 tcctttgatg cgttgccaga gcgcgcgcgg taccagccgg gtgatctgct tttcaccgga
     8581 tcacagtaaa acgttgccag aactgagcgt tgcggcattg acggaaatcg tcaaaacctg
     8641 gcaggagcaa accgcagagc tgggaaaaac gtacccgtgg gtgcaggtct ttgaaaacaa
     8701 aggtgcggcg atgggctgct ctaacccgca tccgcacgga cagatttggg caaatagctt
     8761 cctgcctaac gaagctgagc gcgaagaccg cctgcaaaaa gaatattttg ccgaacagaa
     8821 atcaccaatg ctggtggatt atgttcagcg cgagctggca gacggtagcc gtaccgttgt
     8881 cgaaaccgaa cactggttag ccgttgtgcc ttactgggct gcctggccgt tcgaaacgct
     8941 actgctgccc aaagcccacg ttttgcggat caccgatttg accgacgccc agcgcagcga
     9001 tttggcgctg gcgttgaaaa agctgaccag tcgttatgac aacctcttcc agtgctcctt
     9061 cccctactct atgggctggc acggcgcacc atttaatggc gaagagaatc aacactggca
     9121 gctgcacgcg cacttttatc cgcctctgct gcgctccgcc accgtacgta aatttatggt
     9181 tggttatgaa atgctggcag agacccagcg agacctgacc gcagaacagg cagcagagcg
     9241 tttgcgcgca gtcagcgata tccattttcg cgaatccgga gtgtaagaaa tgagtctgaa
     9301 agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc acaccattca
     9361 ggcgcctggc cgcgtgaatt tgattggtga acacacggac tacaacgacg gtttcgttct
     9421 gccctgcgcg attgattatc aaaccgtgat cagctgtgca ccacgcgatg accgtaaagt
     9481 tcgcgtgatg gcagccgatt atgaaaatca gctcgacgag ttttccctca atgcgcccat
     9541 tgtcgcgcat gaaaactatc aatgggcgaa ctacgttcgt ggcgtggtga aacatctgca
     9601 actgcgtaac aacagcttcg gcggtgtgga catggtgatc agcggcaatg tgccgcaggg
     9661 tgccgggtta agttcttccg cttcactgga agtcgcggtc ggaaccgtat tgcagcagct
     9721 ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac ggtcaggaag cagaaaacca
     9781 gtttgttggc tgtaactgcg ggatcatgga tcagctaatt tccgcactcg gcaagaaaga
     9841 tcatgccttg ctgattgact gtcgttcact ggggaccaaa gcagtttcca tgccgaaagg
     9901 tgtggctgtc gtcatcatca acagtaactt caaacgtacc ctggttggca gcgaatacaa
     9961 cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag ccctgcgcga
    10021 tgttaccatt gaagagttca acgctgttgc acatgagctg gacccaatcg tggcgaaacg
    10081 cgtgcggcat atcctgactg aaaacgcccg caccgttgaa gctgccagcg cgctggagca
    10141 gggcgacctg aaacgtatgg gcgagttgat ggcggagtct catgcctcta tgcgcgatga
    10201 tttcgaaatc accgtgccgc aaattgacac tctggtagaa atcgtcaaag ctgtgattgg
    10261 cgacaaaggt ggcgtacgca tgaccggcgg cggatttggc ggctgtatcg tcgcgttgat
    10321 cccggaagag ctggtgcctg ccgtacagca agctgtcgct gaacaatatg aagcaaaaac
    10381 aggtattaaa gagacttttt acgtttgtaa accatcacaa ggagcaggac agtgctgaac
    10441 gaaactcccg cactggcacc cgatggtcag ccgtaccgac tgttaacttt gcgtaacaac
    10501 gcagggatgg tagtcacgct gatggactgg ggtgcgactt tactttcagc ccgtattccg
    10561 ctttccgatg gcagcgtccg cgaggcgctg ctcggctgtg ccagcccgga atgctatcag
    10621 gatcaggccg cgtttctggg ggcctctatt ggccgttatg ccaaccgcat cgccaatagc
    10681 cgttatacct ttgacggtga aaccgtgacc ctttcgccaa gtcagggcgt taaccagctg
    10741 cacggcggcc cggaagggtt cgacaaacgt cgctggcaga ttgtgaacca gaacgatcgt
    10801 caggtgctgt ttgccctgag ttcagatgat ggcgatcagg gcttcccggg taatctcggc
    10861 gcaacggtgc aatatcgtct gaccgacgat aaccgtatct ccattactta tcgcgccaca
    10921 gttgataaac cttgcccggt gaatatgact aatcacgtct atttcaatct tgacggcgag
    10981 cagtctgacg tgcgcaatca caagttgcag attctggcgg acgaatatct gccggttgat
    11041 gaaggcggca ttccgcacga cggcctgaaa tctgtcgccg gaacgtcttt tgatttccgc
    11101 agcgccaaaa tcatcgccag tgagtttctt gccgacgacg atcagcgcaa agtgaaaggt
    11161 tacgatcacg cattcttgtt acaggccaaa ggcgatggca agaaagtggc ggcgcatgtc
    11221 tggtcagcag atgaaaaatt gcagctgaag gtctacacca ccgctccggc tctgcaattc
    11281 tactccggca acttcctcgg cggcacaccg tcgcggggaa cagaacctta cgccgactgg
    11341 caagggctgg cgttggaaag cgagtttctg ccggacagcc cgaaccaccc tgaatggccg
    11401 caaccggact gcttcctgcg tcctggcgaa gagtattcca gcctgacgga atatcagttt
    11461 attgctgagt aatgttaagc cctcgtcatg agggctttat ctcatattgt tcaaatcacc
    11521 agcaaacacc gacatatttg caactcaata ttcacaacaa ccttacactg cgccactatt
    11581 ttcgctatgg ttatgcgtaa gcattgctgt tgcttcgtcg cggcaatata atgagaatta
    11641 ttatcattaa aagatgattt gaggagtaag tatatggctg taactaagct ggttctggtt
    11701 cgtcatggcg aaagtcagtg gaacaaagaa aaccgtttca ccggttggta cgacgtggat
    11761 ctgtctgaga aaggcgtaag cgaagcaaaa gcagcaggta agctgctgaa agaggaaggt
    11821 tacagctttg actttgctta cacttctgtg ctgaaacgcg ctatccatac cctgtggaat
    11881 gtgctggacg aactggatca ggcatggctg cccgttgaga aatcctggaa actgaacgaa
    11941 cgtcactacg gtgcgttgca gggtctgaac aaagcggaaa ctgctgaaaa gtatggcgac
    12001 gagcaggtga aacagtggcg tcgtggtttt gcagtgactc cgccggaact gactaaagat
    12061 gatgagcgtt atccgggtca cgatccgcgt tacgcgaaac tgagcgagaa agaactgccg
    12121 ctgacggaaa gcctggcgct gaccattgac cgcgtgatcc cttactggaa tgaaactatt
    12181 ctaccgcgta tgaagagcgg tgagcgcgta atcatcgccg cacacggtaa ctccctgcgt
    12241 gcgctggtga aatatcttga taacatgagc gaagaagaga ttcttgagct gaatatcccg
    12301 actggcgtgc cgctggtgta tgagttcgac gagaatttca aaccgctgaa acgctattat
    12361 ctgggtaatg ctgacgagat cgcagcgaaa gcagcggcgg ttgcaaacca gggtaaagcg
    12421 aagtaaacgt cattcgttta aaatgagaaa gccgactgca agtgagtcgg cttttttgtt
    12481 gctaacaatg gagcacattg cctgatgcga cgctgcgcga cttatcaggc ctgtggtgat
    12541 tcatcggata cgccactctg acggcgcatc cgacaattaa accttacccg cgacgcgctt
    12601 ttactgcatt cgccagttga cgtaacagag catcggtatc ttcccagccg atgcaggcat
    12661 cggtgatgct cttaccgtag gccagcggct ccccgctctc gaggctctga ttgccttcca
    12721 ccagatggct ttccaccatc acgccaataa tggccttttc gccaccggca atctgctggc
    12781 aaacgtcagc acaaacatcc atctgctttt tgaattgttt ggacgagtta gcatggctga
    12841 aatcgatcat cacctgtgct ggcaggcctg ctttgttcag cccttctttc acttcagcaa
    12901 cgtgcttcgc gctgtagtta ggctctttac cgccgcgcag aatgatatgg caatcgccgt
    12961 taccgctggt attcacaatc gccgaatgcc cccatttcgt tacggacagg aagcagtgcg
    13021 gcgcaccggc ggcattaatg gcatcgatag ccactttaat cgtaccgtcg gtgccatttt
    13081 tgaagccgac tggacaagaa agccctgatg ccagttcgcg gtgcacctgc gattcggtgg
    13141 tacgtgcgcc aattgcgccc cagctcatca ggtcagcgag atattgtggg gtgatcatat
    13201 cgagaaactc acccgccgct ggcagaccgc tgtcgttaat atcaagcagc aatttacggg
    13261 ctatacgaag accgtcgttg atctggaagc tgttatccat atgcggatcg ttaatcagcc
    13321 ctttccagcc caccgtggta cgcggctttt caaaatagac gcgcattacg atttccagct
    13381 catctttcag ctcttcacgc agcgccagca agcgagtggc atactcttta gccgcgacag
    13441 gatcatgaat tgagcacggg ccaatcacca ccaacaggcg atcatcatta cctttcagga
    13501 tcttatggat cgcttttcgg gcatgggcaa ccgtattcgc ggcattttca gtagcgggga
    13561 atttttccag caatgcgaca ggaggaagta actctttgat ttctttgatg cgtaaatcgt
    13621 cgttctgata attcatgtct gttccagtgt tgccatactt atcttagtga atgcaatact
    13681 tccaatctat atcttccgtc agaatgtgta aacggggttt tacattatga acggattaat
    13741 cctggaatta gcaagaaaaa cgccagattg tcgcgaaaaa cgagatctct cctacaattt
    13801 ctaactgtaa ctcctttgtt tgttaattat ttcaagattc tctgctgcgt ttcataaact
    13861 ggctgaaatc ttaaaccaat gccttatatt cacctgcaaa tgcactgttg gaagaggtta
    13921 tccgacataa cgaccataac aggagcatcc tatgaaaatg acaaaactgg ccacattatt
    13981 tctgactgcc actctaagcc ttgccagcgg tgccgcactg gctgccgata gcggagcgca
    14041 aactaataac ggccaggcaa acgccgcaac tgatgcgggc caggtagccc ctgacgcccg
    14101 tgaaaatgtc gcgccaaata acgtcgacaa taacggggta aataccggtt ctggcggcac
    14161 aatgctgcat tcggatggtt cttcaatgaa caatgacgga atgaccaaag atgaagagca
    14221 caagaacacc atgtgcaaag atggtcgatg tccggatatc aataaaaagg tacaaaccgg
    14281 tgatggcatc aataatgatg tcgataccaa aaccgacggt accacgcagt aaccctgcaa
    14341 aatagccaat aatgggagag cggagtctct cccttttaat ttttcacttc taaaagcagt
    14401 atgatgtcat aaagttaagt gtaagcgtgc agcaagaacc gtattgacgg ggatgtgtta
    14461 ttcagtcggc agtgctacgc gccaggggag cagttcgccg acccggttta tcggccagtc
    14521 ggctatgacg tcaagtacat agcggaggta gctttctggc tccactccgt tcagtttgca
    14581 cgtcccgatc aggctgtaca gcagcgctcc ccgctctcct ccatgatccg aaccgaagaa
    14641 caggtagttt ttgcggccca gactgaccat ccgcaacgca ttttcagcga tgttattgtc
    14701 cgcctcagcc cagccatcat ctgcatagta cgtcagcgcc ggccactggt tcagggcgta
    14761 tgcgaacgct ttcgccagtt ctgagtgtcg cgacagggtt ttcatctttt cacgcagcca
    14821 gctttccagg gatttcagca gcggtttcgt tttcaactga cgttcggcaa ggcgctgctc
    14881 cgccgtcatt ccccttatct ctgcctcgat ggcgtacagt tcgccgatcc gtttcagcgc
    14941 ttcctccgtc agggctgacg gggtgcgaac gtgcacatcg tggattttac ggcgggcgtg
    15001 agcccaacag gcggcttccg ttatccggcc atcccggtac agctcgttga acccggcgta
    15061 tgcatccgcc tgcagtacac cactgaaccc cgcaagatgg gtctgcggat ggatgccttt
    15121 tctgtccggg ctgtaagcga accacaccgc cggcgccagc gttgacccgg cgttacggtc
    15181 gtcacgaacg taggtccata accgcccggt cttcgttttc ttattgcctg gcaacagcac
    15241 cgggacaggc gtgtcatcag catggagctt accgtcagtc agcacatagt cctgaagcgc
    15301 ttcttccagc ggtgacagta gccggcagca tgcatccacc cagcccgaca gcagtgaacg
//
LOCUS       DAJTNA010000087        15344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTNA010000087 DAJTNA010000000
VERSION     DAJTNA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.088"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(250..1362)
                     /gene="ydiK"
                     /locus_tag="OO161_002348"
     CDS             complement(250..1362)
                     /gene="ydiK"
                     /locus_tag="OO161_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5463390.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1751..4807
                     /locus_tag="OO161_002349"
     CDS             1751..4807
                     /locus_tag="OO161_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5463391.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4804..5214
                     /gene="menI"
                     /locus_tag="OO161_002350"
     CDS             4804..5214
                     /gene="menI"
                     /locus_tag="OO161_002350"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5463392.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5314..5502
                     /locus_tag="OO161_002351"
     CDS             5314..5502
                     /locus_tag="OO161_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5463393.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFTDLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6049..6417
                     /gene="sufA"
                     /locus_tag="OO161_002352"
     CDS             6049..6417
                     /gene="sufA"
                     /locus_tag="OO161_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5463394.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6426..7913
                     /gene="sufB"
                     /locus_tag="OO161_002353"
     CDS             6426..7913
                     /gene="sufB"
                     /locus_tag="OO161_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5463395.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRVNAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7923..8669
                     /gene="sufC"
                     /locus_tag="OO161_002354"
     CDS             7923..8669
                     /gene="sufC"
                     /locus_tag="OO161_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5463396.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8644..9915
                     /gene="sufD"
                     /locus_tag="OO161_002355"
     CDS             8644..9915
                     /gene="sufD"
                     /locus_tag="OO161_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5463397.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDAFALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRNNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALCDEGLKQQVLARIGQRLPGGAR"
     gene            9912..11132
                     /gene="sufS"
                     /locus_tag="OO161_002356"
     CDS             9912..11132
                     /gene="sufS"
                     /locus_tag="OO161_002356"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5463398.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAVYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11145..11561
                     /gene="sufE"
                     /locus_tag="OO161_002357"
     CDS             11145..11561
                     /gene="sufE"
                     /locus_tag="OO161_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5463399.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11710..12714
                     /gene="ldtE"
                     /locus_tag="OO161_002358"
     CDS             11710..12714
                     /gene="ldtE"
                     /locus_tag="OO161_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5463400.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDCVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRARQ
                     "
     gene            complement(12778..13014)
                     /gene="lpp"
                     /locus_tag="OO161_002359"
     CDS             complement(12778..13014)
                     /gene="lpp"
                     /locus_tag="OO161_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5463401.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13325..14737)
                     /gene="pykF"
                     /locus_tag="OO161_002360"
     CDS             complement(13325..14737)
                     /gene="pykF"
                     /locus_tag="OO161_002360"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5463402.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15295..>15344
                     /locus_tag="OO161_002361"
     CDS             15295..>15344
                     /locus_tag="OO161_002361"
                     /inference="COORDINATES: protein motif:HMM:NF022418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2767 family protein"
                     /protein_id="HCR5463403.1"
                     /translation="MGNRTKEDELYREMCRV"
BASE COUNT         3864 a         3713 c         4060 g         3707 t
ORIGIN      
        1 gcaattcgtt gtttcactca ggggatttcc atgcttataa atcaatatgt tgatttataa
       61 ccgtgagcta atagtaggca tacggacttt ttgcgtcgat cagattcgtc tcattagttt
      121 aaaaactgta aagaatttgc gacaggtact accagctgaa tggcggtaag caggaaaata
      181 tggggaagag caagcaaata tcaattagcg ggggttaaca atcaggcgac agagccgcct
      241 gatgagaaat tacttattcg gtttctcgat ttcgccaagt tcttcgagaa tttcttccgg
      301 ttggtccgtc ggcggcggga cttcttccac ccacgcggca aacagacgcc aggaaacggc
      361 taacagaacc ggaccaataa acagaccgat catcccgaaa gcaatcaaac caccaataac
      421 accagagagg atcaggatca gcggtaaatc ggcacccatg cgaattaaca ttgggcggat
      481 gacgttatcc agcgtgccaa ccacaccgct ccacactaac aataccgttc cccaggtggt
      541 atcgccagtc cagtagagcc agataatcgc cggaatcagt accggcaacg ggccaagctg
      601 gacaaggcag gagaggatca ttaacaccgt tagcaacgtt gcataaggta cgccggatac
      661 ggcgaggccg ataccgccaa gcactgcctg tactaacgcc gtcaccacca cacccagcgc
      721 caccgcgcgg atagcctgtg ccgccagcag tacggcggca tctccgcgaa cgcctgccag
      781 acgggttgca aaatggcgaa tgccttgtgc cacctgttca ccgcgccagt acagcagggc
      841 actgaagaga agcatcagcg cacaatgcac cataaagcgc ccgatatgcg ccgcctgccc
      901 aacgaaccag gtggtggtgg tgccaatata agggcggact ttcgccatga tcgccgtccc
      961 ccccatatcc agcaagttgt gccagcctgc atacagcttc gcgccaatca ccggaatggt
     1021 attaagccac gccagatcgg gtaacgtcat gtcgccgctg gaaatggctt taattagcgg
     1081 gccgctgccg tcgacgatac tgttaaccag caaagcaata gggatgataa acaccatcac
     1141 taataacagc gtcatcacca gaacggcgag agagcggcgg ccaaacatga tcttttgcaa
     1201 acgtaacaat accggccagg tggcgataac caccgtaccg gcccatgcaa agccgagaat
     1261 aaagggttga acaatccaca gacatgccac aatcatgatg gctaaaaaca gcaccgaaag
     1321 cagaatttgt gcgacatccc tgggctgacg aacatttacc ataactactt ttcacctttt
     1381 ttgtgcgtca aatcgtcgac gcgacgtgaa cacgcgaaac tcacctgcat aatgatgagc
     1441 aatttcagcg gtttttaaca ggccggttct gcccttaatt ctgatgggcg tgttagaaaa
     1501 aaatgtggta caacaactca ggcaacacgc aaacgattta ctcgtcgtat ttcaagttgc
     1561 atgtgctgcg gctacgttcg ttcaccccag tcacttacta ttgtaagctc ctggagttca
     1621 ctcgcttgcc gccttcctgc aactcgaaat ccatagagta aataaacatc acctttcagt
     1681 gcgctattgc agccgcctga aagaatagtg tatcgaccta catcacagac cgcaggaaag
     1741 gggcaatata atgattccac agatttccca ggcacccggc gtcgttcaac tggtgcttaa
     1801 ttttttgcaa gagctggagc aacaaggttt taccggcgat acggcgacaa gttatgccga
     1861 tcgtctgaca atgtcgaccg acaacagtat ttaccaactt ctccccgatg cggtggtatt
     1921 tccgcgttca accgcagatg tggcgctgat cgcccgtctt gccgcgcagg aacgctattc
     1981 atcgctgatc tttacccccc gcggcggcgg caccggcact aacggtcagg cgctcaacca
     2041 ggggattatt gttgatatgt cccgccatat gaaccgcatc atcgaaatta accctgaaga
     2101 gggctgggtg cgcgttgagg ccggggttat aaaagatcaa ctcaatcagt acctgaaacc
     2161 gttcggctac ttttttgcgc cggaactttc gaccagcaac cgggcaacgc tcggtgggat
     2221 gatcaatacc gatgcatccg gtcagggatc gctggtctat ggcaaaacgt cagatcacgt
     2281 acttggcgta cgcgcggtgt tgttgggggg cgatattctc gatacgcaac ctttacccgt
     2341 cgaattggcg gaaacgctgg gtaaatccaa taccacaatc gggcgaattt ataacacggt
     2401 ttatcaacgt tgccgtcagc aacgccagtt aattatcgac aacttcccca aacttaaccg
     2461 ctttcttacc ggttacgatc tgcgtcatgt ctttaacgat gagatgaccg agttcgacct
     2521 gacgcgcatt ctgacgggtt cagaagggac gctggccttt attaccgaag cgcggctgga
     2581 tattacgcgc ttgcctaaag tgcgccgtct ggtgaacgtc aaatatgact cttttgactc
     2641 tgcgctgcgt aacgcgccgt ttatggttga ggcgcgggcg ctttcggtag agacggtgga
     2701 ctcaaaagtg ctgaatctgg cgcgggaaga tattgtctgg cattccgtca gcgagttgat
     2761 taccgatgtg cctgaccaag agatgctcgg gctgaacatt gtggaatttg ctggtgatga
     2821 tgaggcgctg attgatgagc gggtaaatgc actctgtgcg cggttggatg agctgatcgc
     2881 cagtcatcaa gcaggtgtca tcggctggca ggtgtgccgt gagctggcgg gggttgaacg
     2941 tatctatgca atgcgcaaaa aagccgttgg tctgcttggc aatgccaaag gtgccgctaa
     3001 gccaattccg tttgctgagg atacctgcgt accgccggaa cacctggcgg attatattgc
     3061 tgaatttcgc gcgctgctcg acagccacgg cttaagctac ggtatgttcg gtcacgtcga
     3121 cgcaggtgtc ttgcacgtcc gtccagcgct ggatatgtgc gatcctcaac aagagatttt
     3181 gatgaagcaa atctctgatg acgtggtggc gctgactgcg aaatacggtg gtttgttgtg
     3241 gggcgagcac ggcaaaggtt ttcgcgctga atacagcccg gcgtttttcg gtgaggaact
     3301 ttttgcagaa ctgcgcaaag tgaaagcggc atttgacccg cataaccgac tcaacccagg
     3361 gaagatttgc ccgccagaag gtctcgatgc gccgatgatg aaagtggacg cggtgaagcg
     3421 cggtacattc gatcggcaga tccccattgc ggtacgccag cagtggcgcg gtgcgatgga
     3481 gtgtaacggc aacggtttat gcttcaactt tgatgcccgt agtccgatgt gtccgtcgat
     3541 gaagatcacc cagaaccgga ttcattcacc gaaagggcgc gcaacgctgg tgcgtgaatg
     3601 gctgcgtttg ttggcggatc gcggcgttga tccactcaaa ctggaacaag aactgcctga
     3661 atccggcgtc agtttgcgga cgttaattgc ccgcacgcgc aatagctggc atgcgaataa
     3721 aggcgaatat gacttctcac acgaagtcaa agaggcgatg tcgggctgtc tggcctgtaa
     3781 agcgtgttcg accctgtgcc ccatcaaaat agatgtgccg gagtttcgct ctcgttttct
     3841 gcagctctat cacacccgtt atttacgccc gctgcgcgac cacctcgtcg ctacggtcga
     3901 gagctacgcg ccgctgatgg cacgcgcgcc gaaaaccttt aacttcttca ttaaccagcc
     3961 gctggtgcgc aaactctcgg aaaaacatat cggcatggtt gatttgccgc tgctgtcggt
     4021 cccctcgcta caacaacaaa tggtggggca tcgctcggca aacatgacgc tggaacagct
     4081 tgaatctctc aatgcagagc agaaagcgcg cacagtgttg gtggtgcagg acccctttac
     4141 cagctattac gatgcgcaag tggtggcgga ttttgtccgt ctggtcgaaa aattaggttt
     4201 ccagcctgtg ttactgccat tttcgccaaa tggcaaagcc cagcatatta aaggttttct
     4261 taatcgtttt gcgaagacgg cgaaaaagac ggcggatttc ctcaaccgta tggcgaagct
     4321 gggtatgcca atggtgggcg tcgatccggc gctggtactt tgttatcgcg atgaatataa
     4381 actggcgctg ggcgaggagc gtggcgagtt taacgtctta ctggcgaatg aatggctggc
     4441 aagcgcactt gagtcacagc cagtggctac agtcagcggt gaatcatggt atttctttgg
     4501 tcactgtacc gaagttaccg ccttgccggg tgcgccagca caatgggccg cgatatttgc
     4561 ccgttttggc gcgaaactgg aaaatgtcag cgtgggttgc tgcggcatgg cagggactta
     4621 cggacatgaa gcgaaaaacc atgaaaattc gctcgggatc tatgagttat cctggcatca
     4681 ggctatgcag cgactgccgc gtaaccgctg tctggcgacc ggatattcct gccgtagcca
     4741 ggtaaaacgg gttgaaggca cgggggtacg ccatcctgtg caggctttac tggagattat
     4801 taaatgatat ggaaacggaa aatcaccctg gaagcactga atgctatggg tgaaggaaac
     4861 atggtggggt tcctggatat tcgctttgaa catattggtg atgacaccct tgaagcgaca
     4921 atgccagtag actcgcggac aaagcagcct ttcgggttgc tgcatggagg agcatccgtg
     4981 gtactggccg aaagtatcgg ttccgttgcc ggttatttat gtaccgaagg tgagcaaaaa
     5041 gtggttggtc tggaaatcaa tgctaatcac gtccgctcgg cacgagaagg gcgggtgcgc
     5101 ggcatatgta aaccgttgca tctcggttcg cgtcaccagg tctggcagat tgaaatcttc
     5161 gatgagaaag ggcgtttgtg ctgttcgtca cgattgacga ccgccatttt gtgacgttgt
     5221 caccagaaaa gtgtgacgca tattgctttt ggtcattatc tgcacagtaa acggtggtat
     5281 actgtacacg tcttgtctaa aagcgaggat gctatgtcta ctcaacttga tcccacccag
     5341 ctggccattg aatttttacg ccgtgaccag agcaatcttt cacctgccca atatctcaaa
     5401 cgtctgaaac aactagaact tgaatttact gacctgctta ctctctcttc tgctgaatta
     5461 aaagaagaaa tctactttgc ctggcgtctg ggcgtgcatt gatcctgact taatgccgca
     5521 agttcgccaa atcgacctgg attaacctcc gtccaggttc gatgaccttc ttaatactgc
     5581 ttatctaccg cctgatattg ttcaaaaata atactaatcg cagtttgtgt taaaacggcg
     5641 ggttagcttt atgagattat tcttatcgcc ccttcaagag ctaagccact gagagtgccg
     5701 gagataagcg ccggatgggg tagaaaccct taagcctgtg tcgcacagac ttaagggttt
     5761 tcttatttct gaacgctgca cttttttacc tatctcttaa atagtcattt taatacaact
     5821 ttttcttttt catcacagcc ttaatgctcc ttagataata accattatct aacaatgaga
     5881 tacctaattc ttagcgtacc tgttaaccca cacatcaggg tctatgctta ttaaaagcag
     5941 gcaaaaagca tgttttacgg taaagcccct gcgtttgctg ggttgaactg ataatcatta
     6001 tcactaacat gctgttatac gctgaaagcg atgaagtgag gtaaatcgat ggacatgcat
     6061 tcaggaacct ttaacccaca agatttcgcc tggcaaggct taacgctgac acccgcagcg
     6121 gcgatacaca tccgtgagct ggtggcaaag cagccgggta tggtcggcgt gcgcttaggc
     6181 gtgaagcaaa cgggctgcgc gggctttggc tatgtgctcg acagtgttag cgagccggac
     6241 aaagacgatc tgctgtttga acacgacggc gcgaagctgt ttgtcccgct gcaagcgatg
     6301 ccgtttattg atggcacgga agtcgatttc gttcgtgaag gacttaatca gatattcaaa
     6361 tttcacaacc ctaaagccca gaatgaatgt ggctgtggcg aaagctttgg ggtataggcg
     6421 gtactatgtc tcgtaatact gaagcaactg acgatgtcaa aacctggacc ggcggcccgc
     6481 tgaattataa agaaggattc ttcacccagt tagccaccga tgagctggca aaggggataa
     6541 acgaagaggt ggtgcgcgca atttcggcga agcgtaatga gccggagtgg atgctggagt
     6601 ttcgtctaaa cgcctatcgc gcatggctgg agatggaaga accgcactgg ttgaaagcgc
     6661 actacgacaa gctgaattat caggattaca gctactactc agcaccatcg tgcggtaatt
     6721 gtgacgacac ttgcgcgtct gaacctggcg cggtgcagca aactggcgcg aacgcctttt
     6781 taagtaaaga ggtggaggcg gcgtttgagc agttgggcgt tcccgtgcgg gaaggcaaag
     6841 aggtggcggt ggatgccatt ttcgactctg tttcggttgc cactacttat cgcgaaaaac
     6901 tggcggagca gggaattatt ttctgttcct ttggtgaggc gatccacgat cacccggaac
     6961 tggtgcgtaa atatctcggc accgtggtgc cggggaatga caacttcttt gccgcgctta
     7021 atgcggcggt agcctctgac ggtacgttta tttatgtgcc taaaggcgtg cgctgcccga
     7081 tggaactttc cacctatttt cgcgttaacg cggaaaaaac cgggcagttt gagcgcacca
     7141 ttctggtggc cgacgaagac agctacgtca gctacattga aggctgttcc gctccggtgc
     7201 gtgacagcta tcagttacac gcggcagtgg tggaagtcat cattcataaa aacgccgagg
     7261 tgaaatattc cacggtacaa aactggttcc ctggcgataa caacaccggc ggtattctca
     7321 acttcgtcac caagcgtgct ttgtgcgaag gcgaaaacag caaaatgtca tggacgcaat
     7381 cagaaaccgg gtccgcgatt acgtggaaat atcccagctg cattttgcgc ggcgataact
     7441 ccattggtga gttttactca gtggcgctga ccagcggtca tcagcaagcg gataccggca
     7501 ccaagatgat ccacatcggt aaaaacacca aatcgaccat tatctcgaaa gggatctctg
     7561 ccggacatag tcagaacagt tatcgcggct tagtgaaaat catgccgacg gcaaccaatg
     7621 cgcgcaattt cactcagtgc gactcaatgc tgattggcgc taattgtggg gcgcatacct
     7681 tcccgtatgt cgaatgtcgc aataacagcg cacaactgga gcacgaggca acgacatcac
     7741 gtattggtga agatcaactg ttttactgcc tgcaacgcgg gatcagcgaa gaagacgcca
     7801 tctcgatgat tgttaacggt ttctgcaaag acgtgttctc agaactgccg ctggaatttg
     7861 ccgttgaagc acaaaaactc ctcgccatca gtcttgaaca cagcgtcgga taaggaataa
     7921 acatgttaag tattaaagat ttacacgtca gcgtggaaga taaagctatc ctgcgtggat
     7981 taagcctcgc cgttcgtccc ggcgaagttc acgccattat ggggccaaac ggttcgggca
     8041 aaagtacctt atcggcaacg cttgccgggc gcgaagatta tgaagtgacg ggcggcacgg
     8101 ttgagttcaa aggcaaagat ttgcttgcac tgtcgccgga agatcgtgca ggcgaaggca
     8161 tctttatggc cttccagtat ccggtggaga ttccaggcgt cagtaaccag tttttcctgc
     8221 aaacggcact taatgcagtg cgcagctatc gcggccagga aacgctcgac cgctttgatt
     8281 ttcaggattt gatggaagag aaaatcgctc tcctgaagat gccggaagat ttattaaccc
     8341 gttcggtaaa cgttggtttt tccggcggcg agaaaaagcg caacgatatt ttgcaaatgg
     8401 cggtgctgga accggagtta tgcattcttg atgagtcgga ctccgggctg gatattgacg
     8461 cattaaaagt ggtcgccgat ggcgtgaact ccctgcgtga tggcaagcgc tcattcatca
     8521 ttgttacgca ctaccaacgc attctcgact acatcaagcc tgattacgtc catgtgctat
     8581 atcagggacg aattgtgaaa tccggcgatt tcacgttggt caaacaactg gaggagcagg
     8641 gttatggctg gcttaccgaa cagcagtaac gcgctgcaac agtggcatca cttgtttgaa
     8701 gctgaaggga caaaacgctc cccgcaagca cagcagcatt tacaacaatt gctgcgtacc
     8761 ggactgccga cacgtaaaca tgaaaactgg aaatatacgc cgctggaagg gctgaccaat
     8821 agccagtttg tcagcattgc gggggagata tccccacagc agcgtgatgc cttcgcgtta
     8881 acgttagacg ccgtgcggct ggtgtttgtc gatggacgtt acgtgcccgc gctgagcgat
     8941 gcaattgaag gcagcggata tgaagtgagc attaacgatg accgtcaggg gttacccgac
     9001 gctattcagg cggaagtgtt tctgcatttg acggaaagcc tggcacaaag cgtgacgcat
     9061 atcgccgtga agcgcggtca acggccggca aagccattgc tgttaatgta tatcacccag
     9121 ggcgtggcag gtgaagaggt gaacactgcc cattaccggc atcatctgga tctggcggaa
     9181 ggtgccgaag caacggtgat cgaacatttt gtcagcctta atgatgctcg tcattttacc
     9241 ggggcacggt tcactatcaa tgtcgcagcg aacgcccact tgcagcatat caagctggcg
     9301 tttgaaaacc cgctcagtca ccactttgct cataacgatt tgttgctggc tgaggatgcc
     9361 accgcattta gccatagttt cctgctgggt ggcgcagtgt tacgaaacaa caccagtacg
     9421 caactcaatg gcgaaaacag cacgctgcgg atcaatagcc tggcgatgcc ggtgaaaaac
     9481 gaggtgtgtg atacccgtac ctggctggaa cacaataaag gtttttgtaa cagccgacag
     9541 ttgcacaaaa ctatcgtcag cgacaaaggc cgtgcggtat ttaacggttt gatcaacgtc
     9601 gcgcagcacg ccatcaaaac ggatggtcag atgaccaaca ataatctgct gatgggcaaa
     9661 ctggcggaag tggatacgaa accgcagctg gaaatctatg cagatgatgt gaaatgcagc
     9721 cacggcgcga cggtggggcg tattgatgat gaacagatgt tctatctgcg ctcgcgcggg
     9781 atcaatcagc aggatgccca gcagatgatc atttacgcct ttgctgccga actgacggaa
     9841 gcactgtgtg atgaggggct taaacagcag gtgctggccc gaatcggtca acggctgcca
     9901 ggaggtgcaa gatgactttt tccgtcgaca aagtgcgggc cgactttccg gtgctttcgc
     9961 gtgaggtaaa cggtttgccg ctggcttatc tcgacagcgc cgccagtgca cagaaaccga
    10021 gccaggtgat tgacgccgag gccgagtttt atcgtcatgg ctacgcggcg gtgcatcgcg
    10081 gtattcatac cttaagcgcc caggcgaccg agaaaatgga gaacgtgcgc aagcgggcat
    10141 cgctgtttat taatgcccgt tcggcggaag agctggtgtt cgtccgcggc acgacggaag
    10201 ggatcaatct ggtcgccaat agctggggca acagcaatgt gcgggcgggc gataacatca
    10261 tcatcagtca gatggagcac cacgctaaca ttgtcccctg gcagatgctt tgcgcacgcg
    10321 ttggcgcaga gctgcgtgtg atccctctca accccgacgg tacgctgcaa ctggagacgc
    10381 tgcatacgct gtttgatgag aaaactcgcc tgctggcaat tactcatgtc tccaacgtgc
    10441 ttggcacaga aaatccactg gcgggaatga tcacgcttgc gcaccagcat ggagcaaaag
    10501 tgctggtgga tggcgctcag gcggtgatgc atcatccggt ggatgttcag gcgctggatt
    10561 gcgattttta cgtgttttcc gggcataaac tgtatggccc caccgggatt ggcattcttt
    10621 atgtcaaaga agccttgttg caggagatgc cgccgtggga agggggcggt tctatgatcg
    10681 ccaccgtcag cctgagtgaa ggcactacct ggaccaaagc accatggcgg tttgaagccg
    10741 gtacacccaa taccgggggc atcattggtc ttggcgcggc gctggaatat gtttcggtgc
    10801 tggggcttaa taacatagcc gtgtatgaac tgaatctgat gcattacgcg ctatcacagc
    10861 tggaatctgt accgaatctc actctctatg gcccacaaaa caggcttggc gttattgctt
    10921 ttaatctcgg taaacaccac gcctatgatg ttggcagttt tctcgataat tacggcattg
    10981 ctgtgcgtac cggacatcac tgcgctatgc cattaatggc ctattacaac gtccctgcga
    11041 tgtgtcgggc gtcgctggcc atgtataaca ctcatgaaga agtggatcgt ctggtgaccg
    11101 gcctgcaacg tattcaccgt ctgctgggat aacagggagg cactatggct ttattgccgg
    11161 ataaagaaaa attgctgcgt aattttttac gctgcgccaa ctgggaagag aaatatctct
    11221 acattattga gctgggccag cgtctgccag aattacgcga cgaagaccga agcccacaaa
    11281 atagcattca gggctgccag aatcaggtgt ggattgtcat gcgccagaat gcgcagggaa
    11341 ttattgaatt gcagggcgac agcgatgcgg cgattgtgaa agggcttatt gcggtcgtat
    11401 ttattctcta cgatcagatg acgccgcagg atattgtcaa tttcgatgtg cgtccgtggt
    11461 ttgaaaaaat ggcgctcacc caacatctca ccccatctcg ttcacaaggt ctggaagcga
    11521 tgattcgcgc aattcgcgcc aaagccgctg cacttagcta aactaaaggg acagctttca
    11581 tccggttggc tcacattgcc ggatggtcaa tgtttacact gacctgcaca ggagtgtttt
    11641 ggtttcaggt gaacataagc gccatccgac gtttcagcgt gagtctccgg caaatacagg
    11701 aggtttacaa tgaaacgcgc gtctttgctt acactcacgc ttatcggcgc ttttagcgct
    11761 atccaggctg cctgggcggt tgattatccg ctaccaccaa ccggaagccg actggttggg
    11821 caaaatcaaa cgtatacggt gcaagaaggg gataaaaacc ttcaggctat tgcccgacgt
    11881 tttgatactg cggcaatgtt gatccttgaa gccaataaca ctatcgcccc ggtgccaaaa
    11941 cctggtacga cgataactat tccttcacaa ctgttattac ctgatgcacc gcgtcagggg
    12001 attatcgtta accttgcaga gctgcgcctt tattattatc cgccgggaga aaatatcgtg
    12061 caggtttatc cgataggtat tggcttgcag gggctggaaa cgccggtgat ggaaacgcgt
    12121 gtggggcaga aaatccctaa tccaacctgg acgcctacgg caggcattcg tcaacgttcg
    12181 ctggagcgtg gcattaaatt actgccagtc gttcctgccg gaccaaataa cccgctagga
    12241 cgttacgcac tgcgcttcgc gcatggtaat ggcgaatacc tcattcatgg taccagtgcg
    12301 ccggactgcg tcggtttgcg cgtcagttcg ggctgtattc gcatgaatgc accggatatt
    12361 aaagccttat tctccagcgt gcggacggga acgccggtga aagtgatcaa cgaaccggtg
    12421 aaatattccg tggagcctaa cgggatgcgt tatgttgaag tacatcgacc actatcggca
    12481 gaagaacagc agaacgttca gacaatgcca tacacactgc cagcaggctt tacgcaattt
    12541 aaagacaata aggctgtaga tcagaagtta gtcgataaag cgttgtatcg tcgggcaggg
    12601 tatccggttt cggtgagcag tggagcaact cccgcagcca gcaatgcgcc ttcagtagag
    12661 tcagcgcaga atggtgaacc agagcaaggg aatatgttac gcgcgaggca gtagtggtaa
    12721 atagcagaca aaaaaaatgg cgcacaatgt gcgccatttt tcacttcaca ggtactatta
    12781 cttgcggtat ttagtagcca tgttgtccag acgctggtta gcacgagctg cgtcatcttt
    12841 agcagcctga acgtcggaac gcattgcgtt cacgtcgttg ctcagctggt caactttagc
    12901 gttcagagtc tgaacgtcag aagacagctg atcgatttta gcgttgctgg agcaacctgc
    12961 cagcagagta gaacccagga ttaccgcgcc cagtaccagt ttagtagctt tcattattaa
    13021 taccctctag attgagttaa tctccatgta gcgttacaag tattacacaa agttttttat
    13081 gttgagaata tttttttgat gggaaggcac ttatttttga tcgttcgctc aaagaagcat
    13141 cgaacgctgg gttttagata aaaaaattga gagaaaacag gatgcttcca tcggattcat
    13201 cttagataaa gtgagattat atagtgaaat ccgatttgat tttttattgc ttctggttat
    13261 cgattaaata aaaaaagcgc ccatcagggc gcttcgatat acaaattaat tcacaaaagc
    13321 aatattacag gacgtgaaca gatgcggtgt tagtagtgcc gctcggtacc agtgcaccag
    13381 aaaccataac tacaacgtca cctttgtgtg ccagaccgct ctgcagagcc agttctttac
    13441 ccagacggta gaaatcatca gtagaagtga tctctttaac cagctgcggc acaacgcctt
    13501 tgctcagtac caactgatga gccgtttttt cgttggtggt cagtgccagg atggtggcat
    13561 ccgggaagta tttacgtact gcgcgagcag atttaccgcc ctgagtagca accacgatca
    13621 gcggagcatc cagtttttca gcagtttcaa cggcaccacg gcataccgct tcggtaatgc
    13681 gcagtttacg gttgtcattg ttgaactcga gacggctgtt catcacgcgg tcggtacgtt
    13741 cgcagatggt cgccatgata gaaaccgctt ccagcgggta tttacctttt gcggattcac
    13801 cagacagcat cactgcatca gtaccgtcga ggatggcgtt tgcaacgtca ccggcttctg
    13861 cgcgagtcgg gcgtgggttt ttgatcatgg aatccagcat ctgggtcgca gtgataacga
    13921 ctttacgtgc acggatacat ttttcgatca tcatcttctg ggcgaagata acttcttcta
    13981 ccgggatttc tacacccagg tcgccacgcg caaccatgat gccgtcagag gcttcgagga
    14041 tttcgtcgaa gttgttgagg ccttcctggt tttcgatttt ggagatgatg tggatgtttt
    14101 cgccgccgtg cgctttcagg tgctcacgga tttcgataac gtcagaacgc ttacgaataa
    14161 aggaagcagc aacaaagtct acgccttgtt cgcaaccaaa gatcaggtcc tgtttgtctt
    14221 tttcagccag tgctggcaga gcaatggaaa cgccaggcag gttcacacct ttgttttcgc
    14281 ccaggtcacc gttgttcagc actttacaga taactttgtt accttcaatg gcggtaactt
    14341 ccataccgat cagaccatcg tcaaccagta cggtgttgcc aactgacagg tcagtagtga
    14401 aaccttcata cgttaccgca accatttcgc tgttgccgat aacagattta tcagtggtga
    14461 aagtaaaggt ctgaccagct ttcagagaaa cgtcgttacc gccttccagt ttcatggtgc
    14521 ggatttccgg acctttggta tcaagcagga tagcggcggt tttaccagtt ttgctcatca
    14581 cgttgcgcag attctgaatg cgctgaccgt gttctgcata gtcaccatga gagaagttca
    14641 gacgcataac gttcatgcca gcgtccagca ttttagctaa catctcttca gattcggttt
    14701 tcggtccgat ggtgcaaaca attttggtct ttttcatgac agtcttagtc tttaagttga
    14761 gaaggatggg agaaacttgc tttctggacg cactgccgga aagcgaatcc ggtattacga
    14821 aagtggtggt gcctcgctct aaggataggt gacatcgaaa aagcgtgcag aggaatgtgt
    14881 gcttgtgtct cagcccaacg tgagtgcctg tttttgagtc gttgagttct acagtccaaa
    14941 gtgctgaaac cattcaagag tcaattggcg cgcattatac gctgaaatta atccaaaagg
    15001 aaaacaaaaa cagcgtttca aaaaatatac aacttgttag acaataaatt gttatcaaag
    15061 atttaacgtt ccagggaaaa ggttacgcaa aaacttcgct gtttgcgcaa cgtctggtgc
    15121 caaagttgtc agtgtgagga taagtgaaag ttcatccggt gtataaatat atcatcgctc
    15181 aattgctaca gttaaaataa tttatcgcgt tatttgtctg attttgtttg attttttgtt
    15241 taattggtta gattgatata tgctgatagt tggcatgata aataggtata aagaatggga
    15301 aatcggacaa aagaggatga actgtaccga gaaatgtgca gagt
//
LOCUS       DAJTNA010000088        15343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTNA010000088 DAJTNA010000000
VERSION     DAJTNA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15343
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.089"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..789)
                     /locus_tag="OO161_002362"
     CDS             complement(<1..789)
                     /locus_tag="OO161_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5463404.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGCLPRYLAQRFLDSGALIEKKVVAQ"
     gene            complement(835..1659)
                     /locus_tag="OO161_002363"
     CDS             complement(835..1659)
                     /locus_tag="OO161_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5463405.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(1742..1996)
                     /gene="yoeB"
                     /locus_tag="OO161_002364"
     CDS             complement(1742..1996)
                     /gene="yoeB"
                     /locus_tag="OO161_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5463406.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(1993..2244)
                     /gene="yefM"
                     /locus_tag="OO161_002365"
     CDS             complement(1993..2244)
                     /gene="yefM"
                     /locus_tag="OO161_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5463407.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            2527..2577
                     /gene="hisL"
                     /locus_tag="OO161_002366"
     CDS             2527..2577
                     /gene="hisL"
                     /locus_tag="OO161_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5463408.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    2553..2676
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            2723..3622
                     /gene="hisG"
                     /locus_tag="OO161_002367"
     CDS             2723..3622
                     /gene="hisG"
                     /locus_tag="OO161_002367"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5463409.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTHRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRVQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            3628..4932
                     /gene="hisD"
                     /locus_tag="OO161_002368"
     CDS             3628..4932
                     /gene="hisD"
                     /locus_tag="OO161_002368"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5463410.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASGRLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGMADFQKRMTV
                     QELSKEGFSALASTIETLASAERLTAHKNAVTLRVNALKEQA"
     gene            4929..5999
                     /gene="hisC"
                     /locus_tag="OO161_002369"
     CDS             4929..5999
                     /gene="hisC"
                     /locus_tag="OO161_002369"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5463411.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGSVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGIREESQRVIDALRAEQV"
     gene            5999..7066
                     /gene="hisB"
                     /locus_tag="OO161_002370"
     CDS             5999..7066
                     /gene="hisB"
                     /locus_tag="OO161_002370"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5463412.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMLQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            7066..7656
                     /gene="hisH"
                     /locus_tag="OO161_002371"
     CDS             7066..7656
                     /gene="hisH"
                     /locus_tag="OO161_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5463413.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            7656..8393
                     /gene="hisA"
                     /locus_tag="OO161_002372"
     CDS             7656..8393
                     /gene="hisA"
                     /locus_tag="OO161_002372"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5463414.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEDDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            8375..9151
                     /gene="hisF"
                     /locus_tag="OO161_002373"
     CDS             8375..9151
                     /gene="hisF"
                     /locus_tag="OO161_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5463415.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASCDGRVVDKSWVSRVAEVIDIPFCVAGGIMSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDAAVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            9145..9756
                     /locus_tag="OO161_002374"
     CDS             9145..9756
                     /locus_tag="OO161_002374"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5463416.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMSPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKTADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(9853..10830)
                     /gene="wzzB"
                     /locus_tag="OO161_002375"
     CDS             complement(9853..10830)
                     /gene="wzzB"
                     /locus_tag="OO161_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5463417.1"
                     /translation="MRVENNNVSGQNHDPEQINLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRDYNAK"
     gene            complement(10977..12143)
                     /gene="ugd"
                     /locus_tag="OO161_002376"
     CDS             complement(10977..12143)
                     /gene="ugd"
                     /locus_tag="OO161_002376"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR5463418.1"
                     /translation="MKITISGTGDVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKINFNATLDKNEAYRDAGYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIIKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(12392..13798)
                     /gene="gndA"
                     /locus_tag="OO161_002377"
     CDS             complement(12392..13798)
                     /gene="gndA"
                     /locus_tag="OO161_002377"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5463419.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLTGPKAQPAGEKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(13880..14794)
                     /locus_tag="OO161_002378"
     CDS             complement(13880..14794)
                     /locus_tag="OO161_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5463420.1"
                     /translation="MNKEVIAVIVTYNRKALLLKVIDAVINQSYPLKKILIIDNNSTD
                     GTEMFISNRLSDVVKYKNTGDNLGGAGGFYRGFIEAEKYGYDYLWLMDDDFMPTVNCL
                     EILISNNPDGIVQPVRYNLDETCAELSPLTYDLSNPFKLNPKGTPLKNYLNTIGNKSA
                     KVDIEAIPFEGPLISRKVVEKIGYPEPKFFIFCDDIEYAIKAKRKGIPIQCNLKAKAY
                     RLLVNNQGNDLLSWKGYFMLRNLFYLHKTYGTNFLVRQKPIVLGLGYALSCVLKCNFS
                     QLPVIWRAFWDSSTLRNTEKFRPEMRSK"
     gene            complement(14787..>15343)
                     /locus_tag="OO161_002379"
     CDS             complement(14787..>15343)
                     /locus_tag="OO161_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HCR5463421.1"
                     /translation="QYQGIPVGGYNKLIDALLNGIEVQTCTDYFEEKKYWDSIAKKII
                     YTGPIDKFYCYKFGALDYRSLKFEHELLNVDNFQGNAVVNYIDKKFPFTRIIEHKFFE
                     FGEQHNTLITREYPKEFEEGDEYYYPVNDEKNMQIYKMYYNLSKQESNVIFGGRLAEY
                     KYYDMHQVIKSALNAFRKEVRLGE"
BASE COUNT         3910 a         3831 c         3735 g         3867 t
ORIGIN      
        1 ttgggcgacc actttcttct cgattaacgc accactatcg agaaaacgtt gcgccagata
       61 acggggtaaa cagccgcagc cgaggccgct aatttgcagc tccagcttgg ttttaaaatc
      121 gaaaacggta atagcttctt gttcgtcgag aagttgtgag gccgtagaag cgccggcctg
      181 tgcagtgtcc cccaccacaa tcgcccgata tcgtttaatg atacgacggt ttaacggttc
      241 ttcttccagg gctaacggat gatgaggcgc gaccgcaaag acttgttcca gatcgcccaa
      301 ccgagagaaa ccaaactcac tggaagatgg tggctcatgc atcgcgccaa caataatatc
      361 cgctcgcccc tgagtcaggg catcccatga accaccgagt acaccattga taaacttcag
      421 gcgcgtcacg ctatgatgtt gataaaaagc ctcaatgagt ggcgcaagca gagaaaaagg
      481 gaaggtgtcg tccacgccaa tcaccagctc gttttcccag ccttcatgca gtttgatcgc
      541 ctgcttttcc agttctcgca cggtatgcag aacttcccgc cctttctcta ataacatttt
      601 tccggtgcgg gtgaatttag cgcggtgtcc gctgcgatca agcaattgaa tattaaggtc
      661 gctttccagc ttgtgaacgg tgtaactcag agcggacggc gtcttgtaga gtttggccga
      721 cgccgccgca aaactgcctt ctttttctaa ggcatcgagg atcatcaaca catccagcag
      781 tggtttcata cacgccccct tgcagtgtat gcggcgtcct gctggcgtga tcgtttactc
      841 cagcggcatt accagcggat cgggatactg gtattcaaat cccagttcat tacaaatccg
      901 actgccatca ataatcttac ctttgccgct gtccagacta tttctgaact gcggaggttc
      961 cagccccaat aaacgggcca tctgcggata gaaaacatta cgcgcagggt gagctggcgc
     1021 acatatatta tagatgtgtc cgcctttagg tgcctgtaac aacagagtga tagcgccaat
     1081 aacatcttct aaatggacta aattaacacc atgttcacca tcaggggcgg tttttccggc
     1141 aaagaagcgt ccgggatgac gtcccggtcc gaccaggccc gcaagacgca gaatatcgac
     1201 cgaagtaccg ggtaaattgt gcagccagtc ttcgagttct tctaatactc gtccactgtt
     1261 ggtcaccgga ttacgcgggg tggtttcttt caccgtgcct tgcgcgtcgc catagacaga
     1321 tgtcgagctg gtaaaaataa tgcggggaat acgatgggcc agcgcgctat ccactaactc
     1381 ttgtaccgct tgtaaataga actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat
     1441 gaccagcgca tcggcatcca tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg
     1501 ctccatgcga agcagatagc tatcaatgcc actcattcgg gccgcttcga caccatcttg
     1561 tgtggtttta ctcccggtga cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat
     1621 gcctaaccac cctaacccga caattgcgac ctttttcatc cgttatctcc tgatacacct
     1681 tagatctata aggctacgct agcgtatcaa aactgacaat tcattctatg aatgaatctg
     1741 ttcaataatg ataacgacat gctgcaatga gcagtgaatc atcagtaacc gcgtatacca
     1801 gacggtgctc ctctgtaatg cgtcgggacc agaaacctga caaattatgt ttcaggggtt
     1861 ctggtttccc cttaccttca aatggtgttc tgcgggtatc tttgataagt tcattgatct
     1921 ttttaactat tcgcttatct gtttcctgcc agtacagata atcatcccat gattcctcag
     1981 accagattag tttcactcaa taatgtcctt ttccgttcct ttgcctgatt tcaggctatc
     2041 gattgagtcc atcaatctcc gggcgttagc gggggaacgc agtagataag ccgtctcttc
     2101 cagcgagttg tattcttcga gtgacatcag aacacaagcc tctccattct gacgagtaat
     2161 gaggatcggg gcatgatctt caacggcttt catcattgtt gccgacaaat tctgacgcgc
     2221 ttcgctgtag ctaattgtac gcatgtaaat ctcctctttt gtacagttca ttgtacaatg
     2281 atgggtgtta attcactatt tattaattag tttgtagatc aagacattgc cagcgagacg
     2341 aaaaatcaga ctttgctctt tttggtgctg tcaagttaga ggacagtcct cttagccccc
     2401 tcctttcccc gctcattcat taaacaaatc catcgccata aaatatataa aaaagccctt
     2461 gctttctaac gtgaaagtgg tttaggttaa aagacatcag ttgaataaac attcacagag
     2521 acttttatga cacgcgttca atttaaacac caccatcatc accatcatcc tgactagtct
     2581 ttcaggcgat gtgtgctgga agacattcag atcttccagt ggtgcataaa cgcatgagaa
     2641 agcccccgga agatcacctt ccgggggctt ttttattgcg cggttgttaa cggttcagac
     2701 aggtttaaag aggaataaga aaatgacaga caacactcgt ttacgcatag ctatgcagaa
     2761 atccggccgt ttaagtgatg actcacgcga attactggcg cgctgtggca ttaaaatcaa
     2821 tcttcacacc catcgcctga tcgcgatggc agaaaacatg ccgattgata ttctgcgcgt
     2881 gcgtgacgat gacattcccg gtctggtaat ggacggcgtg gtagaccttg ggattatcgg
     2941 cgaaaacgtg ctggaagaag agctgcttaa ccgccgcgtc cagggtgaag atccgcgcta
     3001 ctttaccttg cgtcgtctgg atttcggcgg ctgccgcctt tcgctggcaa cgccggttga
     3061 tgaagcctgg gacggtccgc tctccttaaa tggtaaacgt atcgccacct cttatcctca
     3121 cctgctcaag cgttatctcg accagaaagg tatctctttt aaatcctgct tactgaacgg
     3181 ctctgtcgaa gttgcgccgc gtgccggact ggcggatgcg atttgcgatc tggtttccac
     3241 cggtgccacg ctggaagcta acggcctgcg cgaagtcgaa gttatctacc gctcgaaagc
     3301 ctgcctgatc cagcgcgatg gcgaaatgga agaatccaaa cagcaactga tcgacaaact
     3361 gctgacccgt attcagggtg tgatccaggc gcgcgaatca aaatacatca tgatgcacgc
     3421 accgaccgaa cgtctggatg aagtcatcgc cctgctgcca ggtgccgaac gcccaaccat
     3481 tctaccgctg gcgggtgacc aacaacgcgt agcgatgcac atggtcagta gcgaaaccct
     3541 gttctgggaa acgatggaaa aactgaaagc gctgggtgcc agttcaatcc tggtcctgcc
     3601 gattgagaag atgatggagt gatcgccatg agctttaaca caatcattga ctggaatagc
     3661 tgtactgcgg agcaacaacg ccagctgtta atgcgcccgg cgatctccgc ttctgaaagc
     3721 attacccgca ctgttaacga tattctcgat aacgtgaaag cacgcggcga tgaggccctg
     3781 cgggaataca gcgcgaagtt tgataaaacc acggttaccg cactgaaggt gtctgctgag
     3841 gaaattgccg ccgccagcgg acgcctgagc gacgagctaa aacaggcgat ggcggtggca
     3901 gtaaagaata ttgaaacctt ccacactgcg caaaaactgc cgccggtaga tgtagaaacg
     3961 cagccaggcg tgcgttgcca gcaggtcacg cgcccggtcg cttcagttgg gttgtatatt
     4021 cctggcggct ccgccccgct cttctcaacg gtattaatgc tggcgactcc ggcgcgtatt
     4081 gcgggctgta aaaaagtggt gctgtgctca ccgccgccga ttgccgatga gatcctttat
     4141 gcggcgcagc tgtgcggtgt gcaggacgtg tttaacgtcg gcggcgcaca ggccattgcc
     4201 gcgctggcgt ttggtactga atctgtgccg aaagtggaca aaatcttcgg gccgggtaac
     4261 gcctttgtca ccgaagcaaa acgccaggta agccagcgtc tggacggtgc ggcgatcgat
     4321 atgcccgcag gcccgtcgga agtgctggtg attgctgaca gcggcgctac gccggatttc
     4381 gtggcttctg atttgctttc tcaggctgaa cacggcccgg actcacaggt aattttactg
     4441 acgcccgatg ctgatatggc gcatcaagtt gccgaggccg tcgaacgcca actggcagaa
     4501 ctgccgcgtg ccgaaaccgc ccgccaggca ctgagcgcca gccgcctgat cgtgactaaa
     4561 gatttagcgc agtgcgtaga gatttccaac cagtacggcc cggagcacct gatcattcag
     4621 acccgcaacg cccgcgaact ggtcgatggc atcaccagcg ccggttcggt atttcttggt
     4681 gactggtcac cggaatcggc aggcgactat gcctccggca ctaaccatgt tctaccgact
     4741 tacggttaca ccgccacctg ttccagcctc gggatggcag atttccagaa gcgcatgacc
     4801 gtgcaggaac tctcgaaaga aggcttttcc gcgctggctt cgaccattga aacactggcc
     4861 tccgccgagc gcctgaccgc ccataaaaat gccgttactt tgcgtgttaa cgcccttaag
     4921 gagcaagcat gagcaccgtg actattaccg atttagcgcg tgaaaacgtc cgcaacctga
     4981 cgccgtatca gtcagcgcgt cgtctgggcg gtaacggcga tgtctggctg aacgccaacg
     5041 aataccccac tgccgtggag tttcagctta ctcagcaaac gctcaaccgc tacccggaat
     5101 gccagccgaa agcggtgatt gaaaattacg cgcaatatgc aggcgtaaaa ccggagcagg
     5161 tgctggtcag ccgtggcgcg gacgaaggta ttgaactgct gattcgcgct ttttgcgaac
     5221 cgggtaaaga cgccatcctc tactgcccgc caacgtacgg catgtacagc gtcagcgccg
     5281 aaacgattgg cgtcgagtgc cgcacagtgc cgacgctgga caactggcaa ctggacttac
     5341 agggcatttc cgacaagctg gacggcgtaa aagtggtcta tgtttgcagc cccaacaacc
     5401 cgaccgggca actgatcaac ccgcaggatt ttcgcaccct gctggagtta acgcgcggta
     5461 aggcgattgt ggttgccgat gaagcctata tagagttttg tccacaggca tcgctggctg
     5521 gctggctggc ggaatatccg cacctggcta ttttgcgcac actgtcgaaa gcttttgctc
     5581 tggcgggcct tcgttgcgga tttacgctgg caaacgaaga agtcatcaac ctgctgatga
     5641 aagtgatcgc cccctacccg ctctcgacgc cggtcgccga cattgcggcc caggcgttaa
     5701 gcccgcaggg gagcgtcgcc atgcgcgaac gggtggcgca aattattgca gaacgcgaat
     5761 atctgattgc cgcattgaaa gaaatctcct gcgtggagca ggtttttgac tctgaaacca
     5821 actacattct ggcgcgcttt aaagcctcca gtgcagtgtt taaatctttg tgggatcagg
     5881 gcattatctt acgtgatcag aataaacaac cctctttaag cggctgcctg cgaattaccg
     5941 tcggaatccg tgaagaaagc cagcgcgtca ttgacgcctt acgtgcggag caagtttgat
     6001 gagtcagaag tatcttttta tcgatcgcga tggaaccctg attagcgaac cgccgagtga
     6061 ttttcaggtg gaccgttttg acaaactcgc ctttgaaccg ggcgtgatcc ctcagctgct
     6121 gaagctgcaa aaagcgggct acaaactggt gatgatcact aatcaggatg gtctgggaac
     6181 acaaagtttc ccgcaggcgg attttgatgg cccgcacaac ctgatgctgc agatcttcac
     6241 ctcgcaaggc gtgcagtttg atgaagtgct gatttgtccg cacctgcccg ccgatgagtg
     6301 cgactgccgt aagccgaaag taaaactggt ggagcgttat ctggctgagc aagcgatgga
     6361 tcgcgctaac agttatgtga ttggcgatcg cgcgaccgac attcaactgg cggaaaacat
     6421 gggcattaat ggtttacgct acgaccgcga agccctgaac tggccgatga ttggtgagca
     6481 actcactaaa cgagaccgtt acgcccatgt agtgcgcaac accaaagaga cgcagattga
     6541 cgtccaggtg tggctggatc gcgaaggtgg cagcaagatt aacaccggcg ttggcttctt
     6601 tgatcacatg ctggatcaga tcgccaccca cggcggtttc cggatggaaa tcaacgtcaa
     6661 aggcgacctc tatatcgacg atcaccacac cgtcgaagat accggcctgg cgctgggcga
     6721 agcattaaaa attgctcttg gcgacaaacg cggtatttgc cgctttggtt ttgtactgcc
     6781 gatggacgaa tgccttgccc gctgcgcgct ggatatctct ggtcgcccgc acctggaata
     6841 taaagccgag tttacctacc agcgcgtggg cgatctcagc accgaaatga tcgagcactt
     6901 cttccgttcg ctctcataca ccatgggcgt gaccctgcac ctgaaaacca aaggtaaaaa
     6961 cgatcaccac cgtgtagaga gcctgttcaa agcttttggt cgcaccctgc gccaggccat
     7021 ccgcgtggaa ggcgataccc tgccctcgtc gaaaggagtg ctgtaatgaa cgtggtgatc
     7081 cttgatactg gctgcgccaa cctgaactcg gtgaagtctg ccattgcgcg ccacggttat
     7141 gaacccaaag tcagccgtga cccggacgtc gtgttgctgg ccgataaact atttttaccc
     7201 ggcgttggca ctgcacaagc agcgatggat caggtgcgtg agcgcgaact gtttgatctt
     7261 atcaaagcct gtacccaacc ggtgctgggc atctgcttag ggatgcaact gctggggcga
     7321 cgcagcgaag agagcaacgg cgtcgatttg ctgggcatca tcgacgaaga cgtgccgaaa
     7381 atgaccgact ttggtctgcc gctgccgcat atgggctgga atcgcgttta cccgcaggca
     7441 ggcaaccgcc tgtttcaggg gattgaagac ggcgcgtact tttactttgt tcacagctac
     7501 gcaatgccag tcaatccgtg gaccatcgcc cagtgtaatt acggcgaacc gttcaccgcg
     7561 gcggtacaaa aagataactt ctacggcgtg cagttccacc cggagcgttc tggtgccgct
     7621 ggcgctaagt tgctgaaaaa cttcctggag atgtgatgat tattccggca ttagatttaa
     7681 tcgacggcac cgtggtgcgt ctccatcagg gcgattacgg caaacagcgc gattacggta
     7741 acgacccgct gccgcgctta caggattacg ccgcgcaggg tgccgaagtg ttgcatctgg
     7801 tggatctgac cggggcaaaa gatccggcaa aacgtcaaat cccgctgatt aaaaccctgg
     7861 tggcgggcgt taacgtaccg gtgcaggttg gcggcggcgt gcgtagcgaa gatgacgtgg
     7921 cggcgttact ggaagcgggc gttgcgcgcg tggtggtcgg ctccaccgcg gtgaaatcac
     7981 cagaaatggt gaaaggctgg tttgaacgct tcggtgccga tgccttagtg ctggcgctgg
     8041 atgtccgtat tgacgagcaa ggcaacaagc aggtggcggt cagcggctgg caagagaact
     8101 cgggcgtttc actggaacaa ctggtggaaa cctatctgcc cgtcggcctg aaacatgtgc
     8161 tgtgtaccga tatctcgcgc gacggcacgc tggcaggttc taacgtctct ttatatgaag
     8221 aagtgtgcgc cagatatccg caggtggcgt ttcagtcctc cggcggcatt ggcgacatta
     8281 acgatgttgc tgctctacgt ggtactggcg tgcgcggcgt aatagttggt cgtgcattac
     8341 tggaaggtaa attcaccgtg aaggaggcca tcgcatgctg gcaaaacgca taattccgtg
     8401 tctcgacgtt cgtgatggtc aggtggtgaa aggcgtacag tttcgcaacc atgaaatcat
     8461 tggcgatatc gtgccgctgg caaaacgcta cgccgaggaa ggtgcagacg aactggtgtt
     8521 ctacgatatc accgcttcct gcgatggccg tgtggtagat aaaagctggg tatctcgcgt
     8581 ggcggaagtg atcgacattc cgttctgcgt ggcgggtggg attatgtctc tggaagatgc
     8641 cgcgaaaatt ctttcctttg gcgcggataa aatttccatc aactcccctg cgctggcgga
     8701 cccgacgtta attactcgcc tggcggatcg ctttggcgtg cagtgtattg tggtcggtat
     8761 tgatacctgg tacgacgctg aaaccggtaa atatcatgtg aatcaatata ccggcgatga
     8821 aagccgcacc cgcgtcactc agtgggaaac gctcgactgg gtagaggaag tgcaaaaacg
     8881 cggtgccgga gaaatcgtcc tcaacatgat gaatcaggac ggcgtgcgta acggttacga
     8941 cctcgaacag ctgaaaaaag tgcgtgaagt ttgccacgtc ccgctgattg cctccggtgg
     9001 cgcgggcacc atggaacact tcctcgaagc cttccgcgat gccgccgttg acggcgcgct
     9061 ggcagcttcc gtattccaca aacaaataat caatattggt gaattaaaag cgtacctggc
     9121 aacacagggc gtggagatca ggatatgtta acagaacaac aacgtcgcga actggactgg
     9181 gaaaaaaccg acggactgat gccggtgatt gtgcaacacg cggtatctgg cgaagtgcta
     9241 atgctgggct atatgagccc ggaagcctta gacaaaacca tcgaaagcgg caaagtcacc
     9301 tttttctccc gcactaaaca gcgactgtgg accaaaggcg aaacgtcggg caatttcctc
     9361 aatgtggtga gtattgcgcc ggactgcgac aacgacacgt tactggtgct ggcgaatccc
     9421 atcgggccaa cctgccacaa aggcaccagc agctgcttcg gcaacaccgc tcaccagtgg
     9481 ctgttcctgt atcaactgga acaactactc gccgagcgca aaactgccga cccggaaacc
     9541 tcctataccg ccaaactgta tgccagcggc accaaacgca tcgcgcagaa agtgggcgaa
     9601 gaaggcgtgg aaactgcgct ggcagcaacg gtacatgacc gctttgagct gaccaacgag
     9661 gcatctgact tgatgtatca cctgctggtg ttgttgcagg atcaggatct ggatttaacg
     9721 acggtgattg agaacctgcg taaacggcat cagtgagttg cggggtaagc ggatgcgatg
     9781 ttgttgtcgc atccggcaaa aaaaacgggc aagatgtcac caccctgccc gttttcttta
     9841 atgctgcaaa gattatttcg cgttgtaatc gcgtagagca ttacgcccca acacaatccc
     9901 cgcgccaacc atgccgccca gcagcaccgc cagaatcaag gtgattgcct ttttcggact
     9961 atcgcgacga ataggtaacg tcggtttcat cacataacgg taagcatgaa tatcaagatc
    10021 atcaactttt aagctttcga tatccagcag gttttgacga gtctgatagt agtttggtga
    10081 gaacaccaac ggacgggtcg cctcatgctt aatcatcgac tccagcgctt cgctccccaa
    10141 aaggaacaac gtatcttgcg tcacatcttc agtctgctga acctgtggct ttgtcacctg
    10201 cgcctgattc gcatactgca acgcttcctg aatctgacgg atacgcagat ctttctgctc
    10261 ctgcgcgacc acttcctggg ttttcagaga gtcctgcaag tttttcatac gcaacgcgat
    10321 gttgtctttc aggtcttttt ccagttcttc atttacctgg tcgtcaactt gctggatata
    10381 cttcgccagc tgcttctgcg caccttcagg tgtttcacca acataagaaa ctgctaaagg
    10441 taatgactga tttttgacgg taggctcaat ggtcagcttt tctgcttctt cctggttatc
    10501 cagcgtttcc gctaatgctg agaatgcagt gctgtaacgt ccgataagtg aagcctgaat
    10561 gtcggagact ttcggtacag ccgatccgta gatcacattc atcgcgttgg tataaccagc
    10621 aatttgcccc acatcaggtt gagtaacgat agctgtggaa gtccactttt ctttggcaac
    10681 ggccagatac ccgatagcca gggcaatagc aacaatgaca gagatgatga ttgtcatctt
    10741 gccacgccac aactgcacca gtaaatcaat caaattaatc tgttccgggt catggttttg
    10801 cccagaaaca ttattatttt ctactctcat cattacccta acggacgaaa taattttaag
    10861 tcgccatagt ttagcgataa tcgccatgaa tgctatagga tatatgataa aaataatgaa
    10921 ttattacaaa gggaacataa gagaatcagg gcgaaaatcg ccctgataac aggatgttag
    10981 tcgctgccaa agagatcgcg ggtgtatacc ttatctgcca catccctaag ctcttctgcc
    11041 atacggttgg aaataatgac gtcggcttgt tgtttgaagg tggcgagatc tcgttccagg
    11101 cgagagttga agaatgagtc ttctttcatc actggctcgt atatgatcac ttcaacgcct
    11161 ttagccttga tacgcttcat aatcccctga atggaagacg cgcggaagtt atctgaaccg
    11221 ctctttataa tcagacgata aatacccacc acttgcggct tgcgtgacaa aatggcatcg
    11281 gcaataaaat ctttacgcgt gcggtttgca tcgacaattg ccgaaatcag gttattcggc
    11341 acagactggt agttcgccag taactgcttg gtatctttcg gcagacaata accaccataa
    11401 ccaaacgacg gattgttgta atggttgcca attcgcggat cgagacaaac gccttcgata
    11461 atctggcgag tattcagacc taaactttct gcatagctat ccagttcatt aaagtatgct
    11521 acgcgcatcg ccagataggt attagcgaaa agtttaatcg cttctgcttc ggttgaatcg
    11581 gtgaacagag tcgggatatt ttgcttaatc gcaccttcct gtaacaacgc agcaaaacgt
    11641 tctgcgcgtt ctgaacgctc accgatgaca atacgtgaag gatggagatt atcgtaaagg
    11701 gctttacctt cgcggaggaa ttccggagag aaaataatat tttcagtacg atatttctta
    11761 tgcatcgctg cggtaaaacc aacgggaacc gttgatttga tgaccataac cgcataagga
    11821 tttatttcaa ctacgtcttt aattactgat tctacactgg atgtattgaa ataattagtt
    11881 ttaggatcat agtcggttgg agtagcgatg atgacataac cagcatcccg gtaggcttca
    11941 tttttatcta atgtggcatt aaagtttatt ttatctgatt gcaaaaactg ctgaatttcc
    12001 ttatcaacaa taggagatat ccgatcattc agcatagcaa cgcgtgacgg taaaatatct
    12061 aatgccacaa cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc tacatcgcca
    12121 gtaccggaaa tggtgatttt catttcatgc tctcagaatt aacttaactg tgaatcatga
    12181 tgtttttagc atcctgataa gagctaaaag ttttaacgct acgttgttcg atggtcaacg
    12241 caacgaaaaa caattcagat tttctttata agaatagctg atatttatta agttaatatt
    12301 aagcagtgaa atttagtcta taagataatt ggcaaaaaaa agcccggcac aatatacgcc
    12361 gggcctcaat tttattgtta gttagatcag attaatccaa ccattcggta tggaacacac
    12421 cttctttatc aatgcgctta taagtatgtg caccgaaata gtcacgctgt gcctggatca
    12481 ggttcgcagg cagaacagca gaacggtagc tgtcataata ggcaaccgca gcggcgaagg
    12541 tcggaaccgg gataccgttc tgtactgcgt aagcgacgac atcgcgcagc gcctgctggt
    12601 agtcatcggc aatttgcttg aagtacggag ccagcagcag gttagcgatc tgcggatttt
    12661 cggcataggc atcggtgatt ttctgcagga actgcgcacg gatgatgcaa ccagcacgga
    12721 aaatcttcgc gatttcgccg tagttcagat cccagttgta ctcttcagac gcggcgcgta
    12781 gctgagagaa gccctgagcg taagaaacga ttttgcccag atacagcgca cgacgaactt
    12841 tttcgatgaa ctcagccttt tcgccagctg gctgtgcttt cgggccagtg agaactttag
    12901 acgcggcaac gcgctgatct ttcagagaag agatataacg tgcaaacaca gactcggtaa
    12961 tcagcgacag cggttcgccg agatccagcg cgctctggct agtccattta ccggtacctt
    13021 tgtttgctgc ttcatccaga atcacatcaa ccaggtagtt accgtcttca tcttttttag
    13081 tgaagatgtc tttagtgatg tcgatcaggt agctgctcag ttcaccgtta ttccactcgg
    13141 taaaggtctg cgccagttct tcgttggtga ggttcaggcc acctttaagc agagaatagg
    13201 cttcagcaat cagttgcata tcaccgtatt caataccgtt gtgaaccatc ttcacatagt
    13261 gacctgcacc atcggcacca atataggtaa cgcatggctc cccgtcttca gctactgcgg
    13321 cgattttggt caggatcggt gcaaccagtt cataggcttc tttctggcca ccaggcatga
    13381 tggacggccc tttcagcgcc ccctcttcac cgccggaaac accggtacca atgaagttaa
    13441 agccttctgc agaaagctca cggttacgac gaatggtgtc ctggaagaag gtgttaccac
    13501 catcaatgat gatgtcacct ttatcgaggt atggcttgag agaatcaata gcagcatccg
    13561 tgcctgcacc tgctttcacc attaacagga tgcgacgagg cgtttccaga gattcaacaa
    13621 actctttcac cgtatagtaa ggaaccagtt ttttgcctgg attttcggca atcacttctt
    13681 ccgttttttc acgggaacgg ttgaaaatag agacggtata accacggctt tcgatgttga
    13741 gcgcaaggtt gcgccccatc actgccatac cgacgacgcc gatctgttgc tttgacattg
    13801 tttactcctg tcagggataa atatcgtgtt aagaatttta gtaaaaatcg acattaatta
    13861 ataaaaacat aataataaat tatttacttc tcatttcggg cctgaatttt tcggtatttc
    13921 ttaacgtaga tgaatcccaa aaagcacgcc atataacggg aagttgagaa aaattgcatt
    13981 tcaatacaca cgacaatgca tatcctaatc ccaatacgat aggtttttgt ctaacaagga
    14041 aattggtccc atacgtttta tgcaggtaga aaagatttcg aagcataaaa tatcctttcc
    14101 aggaaagaag atcattacct tgattgttga ccaaaaggcg gtatgccttg gctttaagat
    14161 tgcactggat gggaatcccc tttcttttag ctttaatagc gtattcaatg tcatcacaaa
    14221 aaataaaaaa cttaggctct ggatagccta ttttttcaac aacctttcgt gaaatcaatg
    14281 gaccttcaaa tggaatagct tcaatatcta ccttcgcaga tttattacca attgtattta
    14341 agtaattttt taatggtgta cctttagggt ttaacttaaa tgggttagat aaatcataag
    14401 ttaatggaga aagctctgca caagtttcat ccaagttata tctaacaggt tggacaatac
    14461 catcagggtt atttgatatt aatatttcaa gacaattcac tgtaggcata aaatcatcgt
    14521 ccattaacca aagataatca tagccatatt tttccgcctc tatgaatcct ctataaaacc
    14581 cgccagcccc ccctaaatta tcccccgtat ttttatactt caccacgtca gagagcctat
    14641 ttgaaataaa catctccgta ccatcagtac tattattatc tattatcaat atttttttta
    14701 gtggataact ttggttaata acagcatcta taactttcag caataatgct tttctattat
    14761 aggttacaat aactgcgatt acttctttat tcacctaaac gtacctcttt tcggaatgca
    14821 tttaaagctg atttgataac ttgatgcatg tcataatatt tatattccgc aagacgtcca
    14881 ccaaaaatca cattactttc ctgtttagaa agattataat acattttata gatttgcata
    14941 tttttttcat cattgacagg atagtaatac tcatcgcctt cttcgaattc ttttgggtac
    15001 tctcgagtaa tgagagtatt atgctgttca ccaaattcaa aaaatttatg ttctattata
    15061 cgagtgaatg ggaatttttt gtctatataa tttacaacag cattcccttg aaaattatcg
    15121 acattcagta attcatgctc aaattttaat gatcgataat ccaaagctcc aaatttatag
    15181 caataaaact tatcaatagg ccctgtataa ataattttct ttgcaataga atcccaatat
    15241 ttcttctctt caaaatagtc agtgcatgtt tgaacttcaa taccatttaa taatgcatcg
    15301 attaatttat tgtaaccacc gactggtatt ccttgatact ggt
//
LOCUS       DAJTNA010000089        15074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTNA010000089 DAJTNA010000000
VERSION     DAJTNA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15074
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.090"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            125..382
                     /locus_tag="OO161_002380"
     CDS             125..382
                     /locus_tag="OO161_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCR5463422.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(432..1382)
                     /gene="uspE"
                     /locus_tag="OO161_002381"
     CDS             complement(432..1382)
                     /gene="uspE"
                     /locus_tag="OO161_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5463423.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1534..2286)
                     /gene="fnr"
                     /locus_tag="OO161_002382"
     CDS             complement(1534..2286)
                     /gene="fnr"
                     /locus_tag="OO161_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5463424.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2481..2996)
                     /gene="ogt"
                     /locus_tag="OO161_002383"
     CDS             complement(2481..2996)
                     /gene="ogt"
                     /locus_tag="OO161_002383"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5463425.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIDRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3007..4532)
                     /gene="abgT"
                     /locus_tag="OO161_002384"
                     /pseudo
     CDS             complement(3007..4532)
                     /gene="abgT"
                     /locus_tag="OO161_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            complement(4569..6014)
                     /gene="abgB"
                     /locus_tag="OO161_002385"
     CDS             complement(4569..6014)
                     /gene="abgB"
                     /locus_tag="OO161_002385"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR5463426.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGRISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQTQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSSLK"
     gene            complement(6014..7323)
                     /gene="abgA"
                     /locus_tag="OO161_002386"
                     /pseudo
     CDS             complement(6014..7323)
                     /gene="abgA"
                     /locus_tag="OO161_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
     gene            7499..8407
                     /locus_tag="OO161_002387"
     CDS             7499..8407
                     /locus_tag="OO161_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5463427.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPAQSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            8737..9300
                     /gene="smrA"
                     /locus_tag="OO161_002388"
     CDS             8737..9300
                     /gene="smrA"
                     /locus_tag="OO161_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR5463428.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGVQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(9321..10553)
                     /gene="dgcM"
                     /locus_tag="OO161_002389"
     CDS             complement(9321..10553)
                     /gene="dgcM"
                     /locus_tag="OO161_002389"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR5463429.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            10808..11791
                     /gene="zntB"
                     /locus_tag="OO161_002390"
     CDS             10808..11791
                     /gene="zntB"
                     /locus_tag="OO161_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5463430.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            12268..13641
                     /gene="dbpA"
                     /locus_tag="OO161_002391"
     CDS             12268..13641
                     /gene="dbpA"
                     /locus_tag="OO161_002391"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5463431.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(13770..14705)
                     /gene="ttcA"
                     /locus_tag="OO161_002392"
     CDS             complement(13770..14705)
                     /gene="ttcA"
                     /locus_tag="OO161_002392"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5463432.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILLNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(14757..>15074)
                     /locus_tag="OO161_002393"
     CDS             complement(14757..>15074)
                     /locus_tag="OO161_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5463433.1"
                     /translation="GRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIR
                     RRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWINDMNDEQIAMLNA
                     RLS"
BASE COUNT         3752 a         3700 c         3792 g         3830 t
ORIGIN      
        1 aattaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
       61 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      121 catcatgatt atggcgaaac tgaagtcagc gaaagggaag aaatttctct ttggtttgtt
      181 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      241 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      301 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      361 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      421 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      481 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      541 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      601 aagtgctccg ccaaatcagg aataacctct tcgggcagac ctttttctac gtgcgtcatg
      661 ttttcattaa tgccgaattt ctgccgcagg gctttcattg ccagcaaatg ttgcccacga
      721 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
      781 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
      841 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
      901 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
      961 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1021 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1081 atgatggctt cgaaaggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1141 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1201 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1261 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1321 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1381 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1441 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1501 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1561 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1621 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1681 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     1741 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     1801 cgacaacagc aggatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     1861 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     1921 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     1981 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2041 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2101 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2161 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2221 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2281 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2341 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2401 ctatctattt gaaaaccatt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2461 tggcacaagt tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2521 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggtcaat aacccgatgg
     2581 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2641 ccagggcggc ccaattgttc agccagttgg ccgtaatgca taacctgccc gcaggggata
     2701 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     2761 gtatcaataa tgctaagatt accggcaaaa taatcactaa gcttgtcgct taaaccgcct
     2821 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     2881 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     2941 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3001 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3061 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3121 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3181 aacaaaagga gataccggcg ctaaggcaat acggatgagt cggcaatacg aaagaggatt
     3241 tgcgcaaatg ccgggtgaaa gccaagtagc ataaacattg gtacgaaaat gggggccaga
     3301 atcgaccaga ttgcggaacc actggcaata aacatgcata agaaagagga aagcaacgcc
     3361 agaccgacaa acgccgggat gccgctaagc cctgaacttt ccagtatatc ggtcagcccc
     3421 acggcgatga atttccccat gttgctccag ttaaacatgg cgacaaactg ggcgagggga
     3481 aaaaccatca cgataaatcc cgccatctct ttcatcggtt caatcattaa atgcggtaaa
     3541 tccgcctgat gtcgaattgt gcgggtagcg atgccataag ccagcgaaac cacaaataaa
     3601 aaaagaatga ttagtggcac gataccttta ataaagggtg atggcatcac ggtgtgatta
     3661 atcggatcgc gcaatatccc gttttccggg atcaccatca gcgcgatcgc agcaataaaa
     3721 agtagcgata cgacacctgc tatgcgtaaa ccaaaacgct gactttcggt caatgtctgc
     3781 agtttctcat cgctgtttcc ctgccattga cctaaccgtg gctcgatgat tttgtcggtt
     3841 atcaggccgc caacaatcgt cagtacgact acggagctgg ccataaaata ccagttatca
     3901 attacactga cgtgcatttg cggattgaac gcagccgccg cttccgtgcg gatccccgac
     3961 agcaacacgt cggttgtgac aatcagtaaa ttagccgtaa agccgcaacc tacgcctgca
     4021 atggcagcca gtaaacctgc aactggatgc ctgcccaccg ccagaaaaat cagcgcaccc
     4081 atcggtggca tgatcactaa cgccgcatcg gaagaaatgt ggctgaaaaa agcaataaac
     4141 agcaccatat aactggcgta gcggacatta acatgcgatg ccattttaac cattagcgct
     4201 ggcagtaagc cgacgcgctc cgccagaccg gcacctaaaa ccagcgccag gatcgcacca
     4261 agtggagcaa aaccgctaaa gtttttaata acattgggta aaaaccagtg taatccttcc
     4321 acactgagca ggtttttcac caccaccggc gttccatcgg tcgggttttt cgcactgacg
     4381 ccaaaggccg acaaaattgc tgtcgtcacc atgagtacga taatcaaata gataaagagc
     4441 aaaaaaggat ggggaacctt gttaccaatt ctttcgaccc agccatagag cttcccggat
     4501 tgggaggacg acggtatgga tgacatactc atgggcattc ctcggttgtt gtgtttgcgt
     4561 tgttgttgtt attttaaaga tgacggtgtc acgttttttg ggatagggca gtgatacggt
     4621 tgcgtgtccg taacttgctg atgctcttgt tggcattctt gcaatagccc tgaatcaatg
     4681 aagagattga gtgtggttgc tgccatagtt ttcgccgcca gcagcattcc tttatgagca
     4741 atagatgttc gcccctggct aaccagttgc cacgtatgta acggtgtacc gacggcaaaa
     4801 caggggctga aacactgggc aacaggcagt ttccaactga cgtcgccgac atcagtcgat
     4861 gccgcaagca cgttatcggt ggcggcatat ggagcgacct cattcgccag taccgtttca
     4921 cgatgacgta gtgcaaaagc cttgccgttt tcgccaccgg ttgcggcgat attattcaga
     4981 ctgttttgcc gatcgttggg ggggagcgta gcctgagttt gtttcgtaaa agccagttct
     5041 tcggagttcc attccggggt accaaaatgg gatagggcat ggtacatggc attttctaag
     5101 gtgcgattcg ggagataact ggaacaggct ttgtcgaagc ggcattcaac cgtggtttcg
     5161 gtcatcaatg ccgcaccttc ggcgattttg gcgacccgat cataaatatg ctgcacgtcg
     5221 gtcatttcgg gggcgcggat aagataaagc acttctgcct gcgcctggac cacgttgggc
     5281 gagatcctgc cgctatttgt gatggcatag tgtacgcgcg ctttttcaat aatatgttcg
     5341 ttgaggaagt tggtgccagt ggtcatcaac gttacggcat caagggcgct gcgtcccaaa
     5401 tgaggggaat tcgcggcatg tgctgcgatc cctttaaagc gccatgatgc ctgaatgttt
     5461 gccagcgtgc gggtattgaa cataccggca aaggcttccg ggtgccaggt gagtgccgca
     5521 tccacatcat caaatacccc ctcgcgaacc atgaacgttt tacccgagcc gccttcttcg
     5581 ccaggacaac cataaaagcg caccgtgccg ccttgcccat attgttccag ccatttcttg
     5641 acggctattg cagcggcaaa ggcggcggtt cccagcaaat tgtgtccgca accgtgacca
     5701 ttttcaccgg gcgtcacgga tgtaggttgc gcgcaacctg cttgctgact taaacctgcc
     5761 agggcgtcat attctcccag cagggcgata accggtttgc cttgaccaaa cgaagcaata
     5821 aaggcatttg ggatatagcc tacgttgcgg gtaacggtga agcctgcaga ttccagcgcc
     5881 gaagccagat gctccgctga ccagaactct tcaaaacgtg tttctggatg atcccagatt
     5941 tgatcggcaa tatcggtata acgttggcga tcggcttcaa tcgcatcgtc gataaaacga
     6001 tagatttcct gcatcagata cctcgcgtcc agggaaaatt gagcgcggtg cgcgccagcg
     6061 tttcgacggc aatagcgaga acctgctcgt caaaatcgaa tttttcgttg tgatgacctg
     6121 ccgccagctg tgtgccaaac accacgtagg aggcttgccc ttgatgttgc tgcacgcggg
     6181 ccatcattaa tgtggcatct tcggaacccg caggcgcttc aacacgttca atggcctgat
     6241 tgacccccgc gacctgagcc gcctgacttt gcaaccatgc gacccattgc ggtgaaggag
     6301 aactggcggt agctgcaccc atcagacgag tttcaacgcc gacaccatac atggttgctg
     6361 cgccctgaat cgcttgttgt gcacggtcaa aaacatattg attaatgacg tcgctggccc
     6421 cgcgtgtttc cactttcagc aacgccgagg caggaacaac gttacgaccg cttcctgcct
     6481 gcataacgcc cacgtttact ctggaagctc cttcgctgtg cggggcgatt gcatgcagtg
     6541 caagagtggc ttgtgctgcc gccaacaagg cattgtgacc gtcttctggt tttgcgcctg
     6601 tgtgagcggc ggtaccggtg aagtgcgcgt caaatttggt ggttgccata aaattatcac
     6661 tgccgcacac cacggtgccc gcaggtacgc cagtgccaat gtgcacggca gtaaataatc
     6721 aacatcatct acgacacctg catcgaccat cgcccgcgcg ccacgcgtac cttcctctgc
     6781 aggctgaaaa atcagtttga tgacgcaatg tagtccggac tcgaattgtt taagggtatg
     6841 cgccagccca agcccaatgg cggtatgtcc atcatgacca caggcatgca tcattccggc
     6901 gttacatgac gcaaaaccgt cgcggtaggg gcgatggctg acatcctgct cttcactgag
     6961 atccagcgcg tccatatcga cacggaaagc catcaccgga ccggggcgac cggtatccag
     7021 ggtggcgacg ataccagtga aaccaccttc aaaaaccgca atccattgtg ctagcgcacc
     7081 ctgttgacga gcgcgctcga attcgcgttg tagagtgagt tcatcaggta gtcccatccg
     7141 gctactttca ttaactactt cgcggcccag cgccagtgaa tagccgagct gctgcaattc
     7201 ttccgcaaca agggtggcag tgcggaattc cacccagcca gactctgcat agtgatgaaa
     7261 atcacgtcgc cagtgcgata attttggggc aagcgaatta acaaattgat tcaaagactc
     7321 cataaccttt cccgtcatca gtaaaaagtg tgacccggtt cacgtagcga tagtttttac
     7381 ttatcactaa ctgatttttc acagttttaa ccgttcataa attaccctga cacaatcatc
     7441 tgcattaaag tagatgccag tttctttggt ctgataaata acggttatcg gtggcgtcat
     7501 ggcttttcag gtaaaaattc atcaaattcg tgcttttgtt gaagtggctc gtcagggcag
     7561 cattcgcgga gcgagccgaa tgttgaatat gtcgcaaccg gcacagagta aatctattca
     7621 ggagttggaa gaaggattag cggcgcaact cttttttcgc cgtagtaaag gcgtgacgtt
     7681 aactgatgcc ggtgaaagtt tttatcagca cgccagtctt attcttgaag agctgcgcgc
     7741 agcccaagag gatattcgcc aacgacaagg gcaactggca gggcagatta atatcggcat
     7801 gggggccagt atttcccgca gtctgatgcc agctgtcata tctcgttttc atcagcagca
     7861 tccgcaggta aaagtacgca ttatggaagg gcaactggtg tcgatgatta atgaattgcg
     7921 tcagggagaa ttggatttca ccatcaatac ctattatcag ggaccgtacg accacgaatt
     7981 tacttttgag aaattactgg aaaagcaatt cgcgatcttt tgccgcccgg gacaccccgc
     8041 cattggtgcc cgttcgatca aacagttact ggattacagc tggacaatgc cgacgccaca
     8101 cggcagttac tacaaacagt tgagtgaatt gcttgacgat caggcgcaaa cgccacaggt
     8161 cggtgtagtc tgcgagacgt tctcagcctg tatcagtctg gtggcaaaaa gcgattttct
     8221 cagcatactg cctgaagaaa tgggctgcga tcccttgcac ggacaggggc tggtgatgtt
     8281 gccggttagc gaaattttac cgaaagcggc ctattatttg attcagcggc gtgatagtcg
     8341 ccagacacca ctgaccccgt cattaatcac gcaattccgg cgagaatgcg gctatctgca
     8401 aagttaaaac tgcataaaaa aatagagtct gtcgacatcc gccagactct acagtacaca
     8461 cagcagtgca tccgcgtctt aagtccggca ttgtctcctc tgcgccggtg actgtgttat
     8521 aacggtttta acctttagtt gccaattttc ttcagtgaca gatttcacga aaatataagt
     8581 tacattatta atatcgtgaa tgaataatca tgcataagca ttttgcttaa aatatcggca
     8641 atatttggaa cttattactg gaattttggg taatacgttg ttggaccgac ccggtctggt
     8701 tatcatatcg cgctcttaat tgcgggagga tgtaacatga accttgacga caaatcgctg
     8761 tttcttgacg ccatggaaga tgtccagccg ctgaaacgtg ctacagatgt ccactggcat
     8821 ccaacgcgta accaacgtgc gccgcagcgt atcgacacgc tgcagcttga taatttcctc
     8881 accaccggat ttctcgacat catcccgcta agtcagccgc tggagtttcg gcgggaaggg
     8941 gtgcaacatg gggtgctgga taagctgcgc agtggtaaat atccgcaaca ggcgagcctg
     9001 aatcttttgc gccagccggt ggaagagtgc cgcaaaatgg tgttcagttt cattcaccaa
     9061 gccctggcgg atggtttgcg taatgtgctg attattcatg gtaaagggcg ggaagataaa
     9121 tcgcatgcca atattgtccg cagctatgtg gcgcgctggc tgaccgaatt tgatgatgtt
     9181 caggcatatt gcaccgcgct accgcatcat ggcggcagcg gggcgtgtta cgtcgcacta
     9241 cgtaaaacgg cgcaggcgaa gcaagaaaac tgggagcgcc acgctaagcg cagccgttga
     9301 tctcgagacg catccgcggc ttatgccgcc agcacgcggt tgcgtccatc atttttcgcc
     9361 cgatacaaag catcatcaac gcgtttaaac agttcatcga tgctttcatt tccttcgtga
     9421 tgcgccacac caatgctgac ggtaaaacgt ggtaagcccg aaatactcac tttggccacg
     9481 cttacacgga tagtttcagc cagcgaaagc gcggtatcca gtggggttct tggtagcaat
     9541 aagacaaact cttcgcctcc ccaacgaaac accaaatcgc ctttgcgagc gcaactttcg
     9601 agggtgcggg cgagggcgca taacacctca tcacctttag aatgcccata gagatcgtta
     9661 atgtgtttaa aacgatcggt gtcgatgagc aacaagctgt aatcctgggc gatggcgagg
     9721 tgctgcattt ggcctggttc cgtaatgtga taaaactgtc gccgattcag taatccggtc
     9781 atcgcgtcat ggtgagcagc atgttccagc tgctcctcca gccgtttttg ctcagtaata
     9841 tcatgcacaa tacataacat gagcttgtcg ccataaattt caatcggccc ggcataggtc
     9901 tgcacatgac gagtagatcc atccgccagt ttatgaacaa aattcaaagg tttatgaccg
     9961 ccgggtaaat gcgaaatttc atgcatgata ggcatgacgc gacgcccgag catattaatt
    10021 tcccaggtat gtttctggca catcgtttca tggttataac cgtagaaatt gagcgcggcg
    10081 aggttagcat cgacgatttg gccatctcgt gaggggtcaa tcaacaacat tggtgcagag
    10141 ttagtcagaa aaaagcgcgc ataaaaacct tgttttttgc gctgataatt tgccgagcga
    10201 ctggctttta aacccagcgt tgccggcgct tcgatacctt cgaaaataat caccggttct
    10261 gtttctgtca gctctcgcaa aacaagccga cagcttaatg ctgtttcctc ttctttacgc
    10321 tgaacagtga ggatttcgat aatatcgtgt tggttttgca gatcggagag gtatttcggc
    10381 agttcttttt gtgaggagac ggaatagggt ccggttcgta gctgactaaa cgtgaggtct
    10441 tgcatcaaca gtcgcgccgc gctattggca taaattaact gttcctcaaa gggcgaaaca
    10501 atccagacag gactggtgag taagtccagg gtattgaagt tgtgcgtaat cattgagatc
    10561 ccgttatttt tatcaatttt tgttgctatc cgatcgcaaa aaagccacgt catatgatca
    10621 gataattctg ataatgatag acgctattta acacttcaca cggtttgtat acggaaaagc
    10681 attttgcttt ttgtattcaa tttagacaga attttattaa tcatttcagg gtaatggggt
    10741 gatgagatgt tgcgtaacag ggccagaagg ctagactaca aaataatgcg ttgatgatgg
    10801 aggcactgtg gaagcgatta agggatcgga cgttaatgtc ccggatgcag tatttgcctg
    10861 gatgctggat ggtagaggcg gcgttaaacc gctggaaaat acagatgtga ttgatgaagc
    10921 gcatccctgc tggctccacc ttaattatgt acaccatgat agcgcccaat ggctggcgac
    10981 aacaccgctg cttcccaata acgtacgtga tgcgctggca ggcgagagca cacggccccg
    11041 agtcagccgt ctcggtgaag gcacgctgat tacattgcgc tgtataaacg gcagcaccga
    11101 tgaacgcccc gatcaactgg tcgccatgcg tgtatatatg gacgggcggt taattgtttc
    11161 gacccgacaa cgcaaagtgc tggcgctgga cgatgtggtg agcgatctgg aagagggcac
    11221 gggtccgacc gattgcggag ggtggctggt ggatgtgtgc gatgcgttga ccgatcattc
    11281 cagcgaattt atcgagcagc tgcacgataa aattatcgac cttgaagata atctccttga
    11341 tcagcaaatt ccaccgcgtg gattcctggc tctgctgcgc aaacaattaa tcgtgatgcg
    11401 tcgctatatg gcaccgcaac gtgatgttta tgctcgtctt gccagtgaac gtttgccgtg
    11461 gatgagcgat gaccaacgcc gtcggatgca ggatattgcc gatcgccttg ggcgcggtct
    11521 tgacgaaatc gacgcctgta tagcacggac tggcgtgatg gcggatgaaa tcgctcaggt
    11581 gatgcaggaa aatttagctc gtcgtaccta tacaatgtca ttgatggcaa tggtcttttt
    11641 acccagtacc tttctgacag ggttatttgg cgtcaacctt ggtgggatcc ctggcggcgg
    11701 gtggcaattc ggattttcaa ttttttgtat tctgttagtt gttcttattg gtggtgttgc
    11761 tttatggttg catcgtagta aatggttgta acaaaagcaa tttttccggc tgtctgtata
    11821 caaaaacgcc gcaaagtttg agcgaagtca ataaactctc tacccattca gggcaatatc
    11881 tcccttgcag gtgaatgcaa cgtcaagcga tgggcgttgc gctccatatt gtcttacttc
    11941 cttttttgaa ttactgcata gcacaattga ttcgtacgac gccgactttg atgagtcggc
    12001 tttttttgcc tgttatttat cagcgtctac cctttaagag tccacccaat gaccggaggg
    12061 aaatatgacg acacttattt atttgcaaat tcctgtccct gaaccgattc ctggcgatcc
    12121 tgttccagtg cccgatccga tccctcgccc gcaacccatg cctgacccac cacccgatga
    12181 agaaccgatt aaattgtcgc atcgtgagcg tagatctgcg aggatacgcg cctgctaact
    12241 ttgcgtcgat gaccacgaga atagattgtg accgcttttt ctaccctgaa tgttttgcct
    12301 cccgcccaac tcacgaacct taatgagttg ggttatttaa ctatgacgcc ggtgcaggcc
    12361 gccgcgcttc cggcgatcct tgccggaaaa gatgttcgcg tgcaggcgaa aaccggcagc
    12421 ggcaaaacgg cggcttttgg cctcggcttg ttacagcaaa ttgatgcgtc gctatttcaa
    12481 acccaggctt tagtgctgtg tcctacgcgt gaactggcgg atcaggtggc aggtgaattg
    12541 cgtcggctgg cgcgttttct gccaaatacc aaaattttga cgttgtgcgg tggtcaaccg
    12601 ttcggtatgc agcgtgattc gttgcaacat gcgccgcata ttatcgtggc aacgccgggt
    12661 cgtttgctgg atcacttgca aaaaggcacg gtatcactgg atgcgctgaa tactttggtg
    12721 atggatgagg ccgaccgcat gctggatatg ggatttagcg atgccattga tgatgtcatc
    12781 cgttttgcgc ctgcatctcg acagacgctt ctgttttcgg caacctggcc ggaagccatc
    12841 gccgtaatca gcggacgagt gcaacgcgat cctttggcga ttgaaattga ctcaacagat
    12901 gctttgccac ccattgaaca acaattttat gagacatcca gcaaaggcaa aattcctctg
    12961 ttgcaacggt tattaagctt gcatcagcca tcctcttgcg tggtgttttg caataccaaa
    13021 aaagattgcc aggctgtctg cgacgcgctg aatgaagtag ggcaaagtgc attgtcatta
    13081 cacggcgatt tggagcaacg cgatcgcgat cagaccctgg tacgttttgc taacggtagc
    13141 gcccgtgtac tggttgcgac tgatgttgct gcgcgtggtc tggatattaa atcgcttgag
    13201 ctggtggtga actttgagct ggcgtgggac cctgaagttc atgtacatcg catcggccgt
    13261 acagctcgtg caggaaatag cggtctggcg atcagtttct gtgctccgga agaagcacag
    13321 cgggccaata tcatttctga catgttgcag ataaaactta actggcaaac gccgccagct
    13381 aatagttcca ttgtgccgct ggaagcagaa atggcaacgt tgtgtatcga tggcgggaaa
    13441 aaagccaaaa tgcgcccggg tgatgtatta ggtgcactga caggagatat cgggcttgat
    13501 ggcgcagata ttggcaaaat cgccgtgcat ccggcgcatg tctatgtcgc ggtccgtcag
    13561 gctgttgctc ataaagcatg gaaacagtta cagggcggga agattaaagg aaaaacgtgc
    13621 cgggtgcggt tattaaaata atgaaatgtt gaattgccgg gtgcaagagt aaacatcttc
    13681 ttcggcattg ccggatgcga cgttggccgc gtcttatccg gcctccataa gagtagcccg
    13741 atacgcttgc gcatcgggcg ctatcctggt tatttcactt caaccacatt cagccgtaac
    13801 tcatccaact gattttcatc ttcttctggc tgccagcccg ccggttgtag tgggatctct
    13861 tcgcgatcaa acgccagatc acccccgtta accacttcag aaccgtgggt gatgcctttg
    13921 aaatcgaaca ggttggtatc gcacagatgc gacggcacca cattctgcat cgcgctgaac
    13981 atcgtctcga tacgccctgg ataacgttta tcccagtcac gcaacatgtc agcaatcacc
    14041 tgacgttgca ggttaggctg tgaaccgcac aggttgcacg gaataatcgg gaacgctttt
    14101 gcatcggcaa atcgctgaat atctttctcg cggcagtagg ccagcggacg aataacgata
    14161 tgtttgccat catcgctcat cagtttcgga ggcatacctt tcatcttgcc gccgtagaac
    14221 atatttaaga acaacgtctg caggatatcg tcacgatggt gacccagcgc gatcttcgtc
    14281 gcccccagtt ccgttgcggt acgataaagg ataccgcgac gaaggcgaga acacagtgag
    14341 caagtggttt tgccctctgg aatcttctct ttcacgatac cgtaagtgtt ctcttcgaca
    14401 atcttgtact caacgcccag cttttcaaga tacacgggca gaacgtgttc tgggaagccc
    14461 ggttgctttt gatcgaggtt aacagccacc agcgaaaaat tgattggcgc gctttgctgc
    14521 aaattgagca gaatctccag catggtatag ctgtctttac cccctgagag gcaaaccatg
    14581 atgcgatcgc cttcttcaat catattgaag tcagcaatgg cttcgcccac gttacgacgt
    14641 agacgttttt gtaatttgtt caggttgtat tgttctttct ttgtaatttg ttgattttct
    14701 tgcattattt cagttctctg gtactaaatg gggcaaattg ggggcaaact ttgcaactac
    14761 gataaccgcg cattcaacat ggctatctgt tcgtcgttca tgtcattaat ccacataccg
    14821 taaatttcat acaccatttg cgcagtttca tgccccatct ggctggctat aaatgccggg
    14881 ttcgctcctg ccgtcaacag ccagcaggca aaagtatgtc gcgtatggta cggattacgg
    14941 cggcgaatac cagcacgttt tactgctgca ttccatcttg cacccaaact gcttaccgag
    15001 taataaggtt tctgtttttc gttacacacc ctgggcatga aaacaaaatg cagtttttgc
    15061 ttttcagttc tgcc
//
LOCUS       DAJTNA010000090        14769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTNA010000090 DAJTNA010000000
VERSION     DAJTNA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14769
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.091"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO161_002394"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO161_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5463434.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO161_002395"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO161_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5463435.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO161_002396"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO161_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5463436.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO161_002397"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO161_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5463437.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO161_002398"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO161_002398"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5463438.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO161_002399"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO161_002399"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5463439.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO161_002400"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO161_002400"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5463440.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO161_002401"
     CDS             complement(4598..5320)
                     /locus_tag="OO161_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5463441.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO161_002402"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO161_002402"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5463442.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO161_002403"
     CDS             6520..6738
                     /locus_tag="OO161_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5463443.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO161_002404"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO161_002404"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5463444.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO161_002405"
     CDS             complement(7472..7672)
                     /locus_tag="OO161_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5463445.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="OO161_002406"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="OO161_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR5463446.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="OO161_002407"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="OO161_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5463447.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPCEQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="OO161_002408"
     CDS             10169..12082
                     /locus_tag="OO161_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5463448.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYADRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSNVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="OO161_002409"
     CDS             12082..13104
                     /locus_tag="OO161_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5463449.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="OO161_002410"
     CDS             13098..13316
                     /locus_tag="OO161_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5463450.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIIPRSQFRE"
     gene            13370..14239
                     /locus_tag="OO161_002411"
     CDS             13370..14239
                     /locus_tag="OO161_002411"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5463451.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="OO161_002412"
     CDS             complement(14294..14698)
                     /locus_tag="OO161_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5463452.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
BASE COUNT         3652 a         4039 c         3618 g         3460 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctag tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aataatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa taacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggttcgcc agctcctttg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca caggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgcta gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttatcgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcaaggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caagcccaga gccgcattaa gatgcttgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatgccgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcaa cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggctcg gcataaaccg
    12301 cgtttagtga tatttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta ttccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accccccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctgatgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgta gactggcccc ctgaatctcc
    14761 agacaacca
//
LOCUS       DAJTNA010000091        14759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTNA010000091 DAJTNA010000000
VERSION     DAJTNA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14759
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.092"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..430
                     /locus_tag="OO161_002413"
     CDS             <1..430
                     /locus_tag="OO161_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5463453.1"
                     /translation="RGNHEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALE
                     KCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQVLWSRSRLGERQKGQGIT
                     GADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ"
     gene            complement(431..622)
                     /locus_tag="OO161_002414"
     CDS             complement(431..622)
                     /locus_tag="OO161_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5463454.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(727..963)
                     /locus_tag="OO161_002415"
     CDS             complement(727..963)
                     /locus_tag="OO161_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5463455.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1081..2520)
                     /gene="rsmF"
                     /locus_tag="OO161_002416"
     CDS             complement(1081..2520)
                     /gene="rsmF"
                     /locus_tag="OO161_002416"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR5463456.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     ALKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(2600..5233)
                     /gene="yebT"
                     /locus_tag="OO161_002417"
     CDS             complement(2600..5233)
                     /gene="yebT"
                     /locus_tag="OO161_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR5463457.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPRNKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGGKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5202..6485)
                     /gene="yebS"
                     /locus_tag="OO161_002418"
     CDS             complement(5202..6485)
                     /gene="yebS"
                     /locus_tag="OO161_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5463458.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVELLDSRLLWDAHESGNARFDD"
     gene            6615..7112
                     /gene="msrC"
                     /locus_tag="OO161_002419"
     CDS             6615..7112
                     /gene="msrC"
                     /locus_tag="OO161_002419"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5463459.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIKDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7209..7907
                     /gene="proQ"
                     /locus_tag="OO161_002420"
     CDS             7209..7907
                     /gene="proQ"
                     /locus_tag="OO161_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5463460.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7927..9975
                     /gene="prc"
                     /locus_tag="OO161_002421"
     CDS             7927..9975
                     /gene="prc"
                     /locus_tag="OO161_002421"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5463461.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDAFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPVEQPAPVK
                     "
     gene            10167..11048
                     /gene="htpX"
                     /locus_tag="OO161_002422"
     CDS             10167..11048
                     /gene="htpX"
                     /locus_tag="OO161_002422"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5463462.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11094..12467)
                     /locus_tag="OO161_002423"
     CDS             complement(11094..12467)
                     /locus_tag="OO161_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5463463.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            12644..13435
                     /gene="kdgR"
                     /locus_tag="OO161_002424"
     CDS             12644..13435
                     /gene="kdgR"
                     /locus_tag="OO161_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5463464.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13578..13817)
                     /locus_tag="OO161_002425"
     CDS             complement(13578..13817)
                     /locus_tag="OO161_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463465.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13976..14119
                     /gene="mgrB"
                     /locus_tag="OO161_002426"
     CDS             13976..14119
                     /gene="mgrB"
                     /locus_tag="OO161_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5463466.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14194..14481
                     /locus_tag="OO161_002427"
     CDS             14194..14481
                     /locus_tag="OO161_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5463467.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVILIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3879 a         3590 c         3780 g         3510 t
ORIGIN      
        1 aagaggcaat catgaacaga tggcgatgga tgcgctggca tcccagcaga tgtctttgtg
       61 gttgatgaat ggcggcgact ggtttattgc gctggcagat aatcaacaga aacaagcgaa
      121 aacggcgctg gaaaaatgtc agcatttgcc ctttattctt gaagtacaca gtcgcactgg
      181 caagcatgtt attgctcatg cggattatcc agatgatgtt tatgaatggc aaaaggacgt
      241 tgatttgcat caggtcttgt ggagccgctc gcgattaggt gaacgccaaa aagggcaggg
      301 aattacaggt gctgatcatt tctggtttgg tcatacaccg ttgcgacatc gcgtggatat
      361 tggcaacctg cattatattg ataccggtgc tgtctttggg ggcgaactga ctcttgtgca
      421 attgcaatga ttaaaaatca ccgtactcct gtgcaggtcg ccagaaacca tctataaaat
      481 cctcaatcgg aaaacaaccg ccctggcgga tccgttgatc gctcatagaa taaagacact
      541 gctgttccgt gttgtagaca tccacaacaa tatcttcaca accgccatcc aggtagcaaa
      601 caaaaagtac cagcgcgaac atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag
      661 gagagatttt acaacggggg aataaccagg acaaataacc cgccatgccg acgggttctt
      721 tttggatcag gcaagacgca taatccaggc atcagattgt tgatcgtaac gggtacggta
      781 aaggacagag tgttcgccat ttagcagggc agggatactg gtttcagcat cccaggcatc
      841 gagttgcata cataaatcgg ggtcagattt acaaggaatg gttaacgttc gatcacccgg
      901 cgattcaccg tgtaattcgc cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt
      961 catcttcgtg ctctccattt agctgccggt tgtggtacga ccagtccgat cgcaccattt
     1021 ttcagcctag ttgaggcagc cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg
     1081 tcaggcgtta ccggtaaaaa gtttcccatc gcgcaccagt tcacgcggat agctgttttt
     1141 caatcgcgaa ccaatccgtt tggctaaacc aatcggttga tgctggaaag taaccaacac
     1201 gtcatccgcc actggcgcgg cttgcgggta aacatcgcgc ccgcgatacc actcctccgc
     1261 ttcctgcggt gtcagttcaa aagcgttcac attgtcgggg gtggcaaggg caataactgc
     1321 ttcatgctgc cagcgataac ctttgttgtg cgtttcggca agtttaatcc ccaagcgaga
     1381 aaatcggact ttaccgatca gggcttcaat gcccaccggg aacaaccaca gttctttgtc
     1441 acgctgccag aggcgcaggt tttcatccca gtttaagcca acacctgcag ccgcctggcg
     1501 aatttgtcca gcttcgcgat ctttcaccgg gctgaacgga aaattaccga ctttgtattt
     1561 gggggcgggt aaggcgggaa tcgcctgagt tttacgcaga cgagcaacga aaaagccttc
     1621 gcagtcgtaa atttgtggga aaacatgcaa aaagccttct tcggtcagcg ctttgtttgc
     1681 accagggaag agatcgccaa gtggtaaaaa ctctactgcg tcggggtaag tctctttcag
     1741 ccacaggcaa acggcttcgt tttcttcctg gtttaaggta caggtcgagt aaaccagcgt
     1801 accaccagga cgtaatgcat gaaaggcgct gtcgataagc tcccgttgtg tagctgcgat
     1861 ttcctgattg ctttctggtg accagttttt tagcgcatcg ggatctttac gcaccacgcc
     1921 ttcgccggag cagggagcgt ccagcaaaat ggcatcaaac atttctggca ctgccgcacc
     1981 aaacacgcgg ccatcaaaat gtgtgagcgc aacattactg atgccacagc ggctgatatt
     2041 ggcatgtaac acttttaccc gactggcgga aaactcattg gcaaggattg ccccttcgtt
     2101 attcatccgc gcggcaattt gcgtcgtttt tgagcctggc gcagcagcga catccatcac
     2161 ccgctgtggt gcattaccgt cagcaaacaa ggcggcgacg ggcaacattg aactggcttc
     2221 ctgaatataa aacaggccac ttaaatgctc ggcggtacta cccaatggca atgcatcttc
     2281 attgtcgcgt tcaatccaga aaccttcttc acaccacgga attggcgtaa gcgtccagcc
     2341 ataaggagcg gttaattgca ggaaatcagc aacggagatt ttcagcgcat taacgcgaat
     2401 gctgcggcgc aacgggcgct gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg
     2461 catagcttcg cgcatttgtg tcagaaacgc gtccgggaaa taaacggtgt gttgggccac
     2521 gagcatgtac cactggcaaa aaaacaggcg cgcagtttag cataaacgct ccggcgcagg
     2581 cacgccggag cagtgggcat tatttgggaa gcgcagttcc ccattcacgc cactctttcg
     2641 gttcactttc ctgcaacagg aagtgtttgc cttcctgggc tttcggtgcc agtggcgtac
     2701 ccggaggcgt ggcgaaggcg atgccgccac ggataaactg gttaaaggtg ccggttttca
     2761 ctacgccgcc cgtcagacca aagtccagac tgtaacccga tgccaaccag aagacggaat
     2821 tgttacgcac caggtgttga tagcgtttac tgatgcgcat cgcaatcatc acgcgatctg
     2881 acaatgtccc cagcgtcatt ccggtaaccg tacctacttc cagaccacgg aacagcacag
     2941 gcgtaccaat acctaacgaa ccggcttccg gtgcttcgac aataatgctt aagccatcca
     3001 ggtaacgcga atcagtaata gtagcttctt gcaactcaaa gtcacggcga ggattacccc
     3061 gacctggttc gacattgata tacggctgga ggatagtatc tagatgctca acgcccgcgg
     3121 ctgaaatttg cggtgtgacc accgagaagc gcgtaccgcc gcgggcgaag gtctggacat
     3181 actccggata gagcaccgcc tttgcctgta cttcattgcg cgcggtaatc agatccagcg
     3241 tctggatttg cccgatatca ataccgagat agcgaattgg catcccgacc gccagttttc
     3301 cggcatcgaa agcgtgaagc gtaatctgcc caccaaccgc acgggccgct gtttcggaag
     3361 catacagaat tcgtttgtcg cctttacgct gactggcgct ggcaccgctg aggttatcga
     3421 agctaatggc tccctttaat gctctggaga gcggggatgc ctgtacggtc aggccactac
     3481 cattcagctg aactttcccc ccgccttctg cccagaacac gctattgctg gtcagaaggt
     3541 tgcgatactc cggcttaata tgcagatcga tatcaaacgc gttagctcgc ggacgcacgg
     3601 taatcacttc accaacttca aatttacggt acagcactac cgatcctgcc tgcacatccg
     3661 gcagcgtctc tgcactcaaa ctcacggttg tggtgggtaa atcgctaagg ctgttctcca
     3721 gcgctttttc cagattggca tacagtggat agctggcttt catctcacct ttattgcgtg
     3781 gcagaatacg tatcccgccg ttaatccatt ctgaggcgct ggcaccaaga aactcaacgc
     3841 catccagccc caccttcacg tcgacacggc tgttgacgac aaatttgcta tcgcctttta
     3901 ccagttcgcg atgctgaggc tcgatggcga cggtaaaggt aacgcctttg ctggtgagtt
     3961 tacgatcaat aacctggcct acctgcacgc cgtgaagaat gagcggctga cccgtatcaa
     4021 taccgtaact ttccggcgcg gtcagggtca gcgtcagaac atcaggttca tgcagcagtg
     4081 ctttttcgcc tggcacaaca acgaactctt tgcgtggctc gccatcgccg ggtaccaact
     4141 cgaaggtttt gccagtcagc agggcgctga gattggcatc gctaagggat aatttcggat
     4201 tacgtaattc gatgcgggta ttttcacgaa gtagggtaac gacgctggga tcaactgtca
     4261 tttccccagt gactttacca ccaggattta aatccagttt agttagctgt ccgacttcca
     4321 gtccctgata cattaatggc gtcgagtcgg cggttaatcc ggccccactc ggcagttcca
     4381 gtttgattat tacgccacgc tggctatggg cgaggtcttc atacagacca aaggtatctt
     4441 ccgcctcggc aggtttcgac tcttctggtg aatcgaaggc aatcgcaccg ttaaccagtg
     4501 ccgccaggct ttccagtttc accttcgcgc cactgatact gacattggca tcaacgccgg
     4561 aaacgttcca gaaacggcta ccttttttca ccagatcggt aaaacgccgc tcgatcagga
     4621 catcaatcac cacgccttgt ttgttgggat tgatggcata gtcgtagact tttcccaccg
     4681 ggatcttgcg gaaatagacc aatgaaccgc tgttcagcga accgagatcg ggggcttgca
     4741 ggtggatcat cagatcgcca ttgtccagcc gatatttcgg ttgggtatcg agtgcgacaa
     4801 agtgatcctg ctctttacct gtacccggca tcatgccgat atagttccca ccgacgaggg
     4861 cgtccagccc ggagacacct gccaacgatg cttttggcgt caccagccag aattgagtct
     4921 cttcgcgcag cgcatctttc atatcggact tgatgctgac cttgacttca atcttacgaa
     4981 gatcgtcgct gaggctgata tcctgcactg ttccgacttc aacgccctga taacgaacag
     5041 gcgtacggcc cggtacaata ccatccgccg acataaagtc gatggtgacg gtattacttc
     5101 ggtcctgata actgtcccaa atcagccaac cggcaatcat tagcgcgatg aaaggcagca
     5161 gccagaaagg tgagatacgg cgtttatttt taatctgcgc ttcagtcgtc gaagcgggcg
     5221 tttcctgact catgtgcatc ccaaagtaag cggctgtcca gcaattccac agcaagaata
     5281 gtcaaaatta cggctgcgcc aaaataaaac gcagccggtc ccatagtaaa agcgaggatc
     5341 tgatcgcgat taatcagcga catggttaaa gatatgacaa acaggtcgag catcgaccag
     5401 cgtccaatcc aggtcaccat ccgcagtaac agaatgcgtg tgcgtaaacc ttgctggcat
     5461 ttaaaatgaa tgctcaacag taaagtgaac atgacgatca ctttagtaaa cggtaccaga
     5521 atactggcga taaacacgat tccggcgacc gcaatgttgc tacttgccag cgacataatc
     5581 ccggaaagaa tcgtatcttc ctgccggcca ccattcagat aaatgataga aataggcaat
     5641 aggttggctg gtaacaacaa aacgattgac gctaacagcg ccgcccagca tttttgcaaa
     5701 ctatgacgac gacgcaggcg tagcgggata tggcaacgcg ggcagcgacc acgctgatca
     5761 ggatagccgg taaaatggca cccaagacag acacgaagtt tctcgtccct acgcgtagcg
     5821 gggcgctgcg gataatatcg ctcccacagc tcctcaacat taagatgtga caacgtcacc
     5881 gtcgttaaaa tcaccaacgc cacaaaagag aacaagccga cacccgcctg gatatgggca
     5941 taatcctgta cctttataga agcaacgcca atgccgacca ggtagatatc cagcattacc
     6001 cactctttaa gacgctcaag catcagcagt accggacgta aattcattcc cagtcggtta
     6061 ccaaaccata aatatgctat ggaggacacc agaatgaggg gggcaccaat aacgcagaaa
     6121 aagaccatcg cccccgttat cgtatcgccc tgtttggtca tttgccagat gccttgcatg
     6181 acgtttgcgt cgatacgaat acctaacagc cagatatgca acagcggttc gccccaggca
     6241 aacggcatca gcaacagcat ggtgaaagcc attgccgcca ggcgcgttag cgaccagtcg
     6301 cgcccgtcgc gaatttttgc ctgacagcgc ggacaatagg cactctgatg agaatttatc
     6361 tcgggcaggc taaacagcat gtcacattgc gggcaacgtt ggtaatcacc acgcggcagt
     6421 tcctcgccga ttgacctcac tgttatcttt tttgtcggcg taatttgtgg tgtgttaaga
     6481 gccatgacgt gccaaacgta ataagaatta acattaatct taacccatga tgggaatgat
     6541 cttgagcatt aacgcattta atgcttattt taataggctg gcagttccag gtaagacaaa
     6601 caagtgatta tataatgaac aaaacagaat tttacgcgga tttaaatcgc gactttaacg
     6661 cgctgatggc gggagaaacc agttttctgg caacgcttgc gaacaccagt gcgttgttat
     6721 acgagcgtct cactgacgta aactgggcag gtttttattt gcttgaggac gatacactgg
     6781 tactcggacc atttcagggc aaaattgcct gtgtccggat acccgtcggg cgcggggtgt
     6841 gcggcactgc ggttgcccgc aatcaagtgc agcgtatcaa ggatgttcac gcgtttgacg
     6901 ggcatattgc ctgtgatgcg gcgagtaatt ctgaaattgt tctgccgctg gtggtgaaaa
     6961 atcagattat tggtgttctt gacatcgata gtaccgtctt cggtcgcttt acagacgagg
     7021 acgagcaagg cttacgtcag cttgtggcac agcttgaaaa agtgcttgca acgacggatt
     7081 acaaaaaatt ctttgcgagc gtcgcaggat aatcaacgga taacgtagca tttactggta
     7141 gcgtcattat aatgacgcct gttcatgcct gcgcttgttg gctacgtccg ttgtaatcag
     7201 gaaatttcat ggaaaatcaa cctaagttga atagcagtaa agaagtaatc gcgtttctgg
     7261 ccgaacgttt tccccactgt ttcagtgcgg aaggtgaagc gcgtccgctg aaaatcggta
     7321 tttttcagga tttggtcgat cgtgttgccg gggaaatgaa cctgagcaaa acgcaattac
     7381 gatccgcttt acgtctctac acttcgagct ggcgttatct ttacggtgtt aaacccggcg
     7441 caacgcgtgt cgatcttgac ggcaacccat gcggtgagct ggacgagcaa catgtagagc
     7501 atgctcgcaa gcagcttgaa gaagcgaaag cgcgtgttca ggcacagcgt gctgaacagc
     7561 aagcgaaaaa acgcgaagct gccgcagctg ctggtgagaa agaagacgca ccgcgccgcg
     7621 aacgcaagcc acgtccgacc acgccacgcc gcaaagaagg cgctgaacgt aaacctcgtg
     7681 cgcaaaagcc ggtagagaaa gcgccaaaaa cagtaaaagc acctcgcgaa gaacagcaca
     7741 ccccggtttc tgacatttca gctctgactg tcggacaagc tctgaaggtg aaagcgggtc
     7801 aaaacgcgat ggatgccacc gtattagaaa tcaccaaaga cggcgtccgc gtccagctga
     7861 attcgggtat gtctttgatt gtgcgcgcag aacacctggt gttctgaaac ggaggccggg
     7921 ccaggcatga acatgttttt taggcttacc gcgttagctg gcctgcttgc aatagcaggc
     7981 cagaccttcg ctgtagaaga tatcacgcgt gctgatcaaa ttccggtatt aaaggaagag
     8041 acgcagcatg cgacggtaag tgagcgcgta acgtcgcgct tcacccgttc tcattatcgc
     8101 cagttcgacc tcgatcaggc attttcggcc aaaatctttg accgctacct gaatctgctc
     8161 gattacagcc acaacgtgct gctggcaagc gatgttgaac agttcgcgaa aaagaaaacc
     8221 gagttaggcg atgaactgcg ttcaggcaaa ctcgacgctt tctacgatct ctacaatctg
     8281 gcgcaaaagc gccgttttga gcgttaccag tacgctttgt cggtactgga aaagccgatg
     8341 gatttcaccg gcaacgacac ttataacctt gaccgcagca aagcgccctg gccgaaaaac
     8401 gaggctgagt tgaacgcgct gtgggacagt aaagtcaaat tcgacgagtt aagcctgaag
     8461 ctggcaggaa aaacggataa agaaattcgt gaaaccctga cacgccgcta caaatttgcc
     8521 attcgtcgtc tggcgcaaac caacagcgaa gatgttttct cgctggcaat gacggcgttt
     8581 gcgcgtgaaa tcgacccgca taccaactat ctttccccgc gtaataccga acagttcaac
     8641 actgaaatga gtttgtcgct ggaaggtatt ggcgcagtgc tgcaaatgga tgatgactac
     8701 accgttatca attcgatggt ggcaggtggt ccggcagcga agagtaaagc tatcagcgtt
     8761 ggtgacaaaa ttgtcggtgt tggtcaaaca ggcaagccga tggttgacgt gattggctgg
     8821 cgtcttgatg atgtggttgc cttaattaaa gggccgaagg gcagtaaagt tcgtctggaa
     8881 attttacctg ctggtaaagg gaccaagacc cgcactgtaa cattgacccg tgaacgtatt
     8941 cgtctcgaag accgcgcggt taaaatgtcg gtgaagaccg tcggtaaaga gaaagtcggc
     9001 gtgctggata ttccgggctt ctatgtgggt ttgacagacg atgtcaaagt tcaactgcag
     9061 aaactggaaa aacagaatgt cagcagtgtg atcatcgacc tgcgtagcaa tggcggtggg
     9121 gcgttgaccg aagcggtatc gctctcaggt ctgtttattc ctgcgggtcc cattgttcag
     9181 gtccgcgata acaacggcaa ggttcgtgaa gacagcgata ccgacgggca ggtgttctat
     9241 aaaggccctc tggtggtact ggttgaccgt ttcagtgctt cggcttcaga aatctttgcc
     9301 gcggcaatgc aggattacgg tcgtgcgctg gttgtgggtg aaccgacgtt tggtaaaggc
     9361 accgttcagc aataccgttc attgaaccgt atttacgatc agatgttacg tcctgaatgg
     9421 ccagcgctgg gttctgtgca gtacacgatc cagaaattct atcgcgttaa cggcggcagt
     9481 acgcaacgta aaggcgtaac gccagacatc atcatgccga cgggtaatga agaaacggaa
     9541 acgggtgaga aattcgaaga taacgcgctg ccgtgggata gcattgatgc cgcgacttat
     9601 gtgaaatcag gcgatttaac ggcctttggg ccggagctgc tgaaggaaca taatgcgcgt
     9661 atcgcgaaag atcctgagtt ccagaacatc atgaaggata tcgcgcgctt caacgctatg
     9721 aaggacaagc gcaatatcgt ttctctgaac tacgctgtgc gtgagaaaga gaataatgaa
     9781 gatgatgcga cgcgtctggc gcgtttgaac gaacgcttta aacgcgaagg taaaccggag
     9841 ttgaagaagc tggatgatct accgaaagat taccaagagc cggatcctta tctggatgag
     9901 acggtgaata tcgcactcga tctggcgaag cttgaaaaag ccagacccgt ggaacaaccc
     9961 gctcccgtca agtaatatca atcaggcaca agaaattgtg cctgattttt taacagcggc
    10021 aagatgccgt aaatcagatg ctacaaaatg taaagttgtg tctttctggt gacttacgca
    10081 ctatccagac ttgaaaatag tcgcgtaacc catacgatgt gggtatcgca tattgcgttt
    10141 tgttaaactg aggtaaaaag aaaattatga tgcgaatcgc gctcttcctg ctaacgaacc
    10201 tggccgtaat ggtcgttttc gggctggtac tgagcctgac agggatacag tcgagcagcg
    10261 ttcaggggct gatgatcatg gccttgctgt tcggttttgg tggttccttc gtttcgcttc
    10321 tgatgtccaa atggatggca ttacgatctg ttggcgggga agtgatcgag caaccgcgta
    10381 acgaaaggga acgttggctg gtcaatactg tagcaaccca ggctcgtcag gcagggatcg
    10441 ctatgccgca agtggctatc taccatgcgc cggacatcaa cgcttttgca accggtgcgc
    10501 gccgtgatgc ctctctggtt gctgtcagca ccggtttgct gcagaacatg agcccggatg
    10561 aagccgaggc ggtaattgct cacgaaatca gccacatcgc caatggtgat atggtcacca
    10621 tgacgctgat tcagggcgtg gtgaacacct tcgttatctt tatttcccgt attctggcgc
    10681 agcttgccgc gggttttatg ggcggaaatc gtgatgaagg tgaagagagc aacggcaacc
    10741 cgctgatcta ctttgcggtt gcaacggttc tggaactggt gtttggtatt ctggcgagca
    10801 ttatcaccat gtggttctcg cgtcatcgtg aattccatgc tgatgccggt tcggcaaaac
    10861 tggttggtcg cgagaaaatg attgccgcgc tgcagcgcct gaaaaccagc tatgaaccgc
    10921 aagaagcaac cagcatgatg gctttctgca ttaacggtaa gtcgaaatcg ctcagtgagt
    10981 tgttcatgac tcacccgccg ctggataaac gaattgaagc tctgcgtacg ggtgaatacc
    11041 tgaagtaata tgaagtaaat actaaaaagt gcgtcctgat cgacgcgctt tttttatgcc
    11101 ctggatcgtg gctgagtgat acgtaaacca ctgacacagg cagcaatcac tgccagaata
    11161 gccgcagcca tcagcgaaac gtgcgtacca ttatcaccga actgatttag catcagcgcc
    11221 accagtgccg cgccgctact ctgacccagt agacgagcag ttcctaacat gccactggct
    11281 ccaccgctac gttcgcgagg agcggaggta ataatggtgt ggttattggg tgactggaac
    11341 aagccaaatc cagcgccaca taagatcatc ggccagataa tattgatatc cgcaggtgac
    11401 gcgggcagca gaaccaggga aaaaagcccc gcagccatga tgaacaaccc tagagcaccc
    11461 agcaatcctg catgtacgcg ttcaatcaaa tagcctgcca acggagccat caccatcgtt
    11521 gctaacggcc acggtgtcag aagtaaacct gtttcgactt cactacgccc gagcacggtt
    11581 tgcaggtaaa agggcaggga aaccattgcc agcatttgtg cgcagaaaga gcaaacagat
    11641 gtgcaaatag aaagtgaaaa cagcgggata cgcagtaaat ccaccggtag cagcggtacg
    11701 ggaagagaaa gctggcggcg aataaagaaa ataccaacaa caaccattac caccagttcc
    11761 gcagcaatta acgtcagcga ttgcccctga gcgaaaccac tcaacgcagt gataagcagg
    11821 ccgaaggtta acgcgttcat cacggcgctg ggcaggtcga aacggggttt actggcgcga
    11881 gaaccattgg gtggcagaaa acgcatcgcc agaagcaggg cgataatacc taacggtacg
    11941 ttgattaaaa ataaccattt ccaggatgcg atggagagga ttgctgcagc aattgtcggc
    12001 ccggcagcag aagagacggc aacaataaac gagtttatgc ccatccctct ccccagaaaa
    12061 cgttgtggat agatcaggcg gataagtgcg gtattaacgc tcatcaacgc tgcaccgccg
    12121 aaaccttgta tgacacgcgc aagggtgagc atttgcagcg aatcagaaag ggcgcagaac
    12181 agtgaagaca acagaaaaac gaccagaccg catttataaa tacgtcgata gccaaacata
    12241 tcgcccagaa acgaaaacga gagcagggag atgacaatgg cgatttgata ggcgttcact
    12301 acccagatgg aactggctgg cgtggcatga aggtccgtgg cgattgttgg cagggcgacg
    12361 ttggcgattg cgccgtcaag gacagccatc gaaataccaa tcacaatggt taatatcgca
    12421 ccgtatcgct ggggcaatgg caggccgtcg gcctgaactt ttggcatagg aattttatat
    12481 ctttggtgaa taatcaacag gggtatgatt ctagcactga tattcacggt ggatgtcgca
    12541 gaattgtaac taaccggcaa aagatggatt gcgacccccc tggtgcgaaa ttataataaa
    12601 aaacagttct gatttttata aaacactcgc aatgaggtga taaatggcta acgcagatct
    12661 ggataaacag cctgattctg tatcttccgt gctaaaagtt tttggcattt tgcaggcgct
    12721 gggtgaagag cgcgaaatag ggataaccga gctgtcgcag cgcgtcatga tgtcaaaaag
    12781 caccgtttat cgctttttac agaccatgaa aaccttaggt tatgtggcgc aggaagggga
    12841 gtcggagaaa tattccctga ccctgaaatt gtttgaactg ggcgctcgcg cgttacaaaa
    12901 cgtcgattta attcgtagcg cagatatcca gatgcgtgag atctcccgcc tgaccaaaga
    12961 aactatccac ctcggcgcac tggacgaaga cagtattgtt tacattcaca aaattgactc
    13021 tatgtacaat ttgcgcatgt attcacggat tgggcgtcgt aatccgctgt acagcaccgc
    13081 gattggtaag gtactgctgg catggcgcga tcgcgatgaa gtgaagcaaa ttcttgaggg
    13141 cgtggagtat aaacgcagta ccgagcggac catcaccagt acagaagcgt tattacccgt
    13201 tctggaccag gtgcgcgagc aggggtatgg cgaagataac gaagagcagg aagaagggct
    13261 gcgatgcatt gcggtaccgg tatttgatcg ctttggcgtg gtcattgccg gtttgagcat
    13321 ctccttcccg acgttgcgtt tctctgaaga gcgtttacag gaatatgtcg caatgttgca
    13381 taccgcagcg cgcaaaattt ctgcccaaat gggttatcac gactatccgt tctgatgaga
    13441 gtaagaacct gtcggaatat caaacagaca ggttctttat ttagcatgag aaaaataaag
    13501 ttgaaggtgc gttatattaa acgcgcttgc tataagaata ttttactcat gagtgagaat
    13561 ctggttattt attaccctta accattatcg accacgatat tgcttttacg taacagtggg
    13621 caatctgtta tccccaagaa accactttta gtgtgcaagt attgtaccgt gctggtgcct
    13681 ctggcagtca gataggtaca ttgcaaacct aatcctgcgg cattctcttt gcttccaatc
    13741 aaaacgccat atccgctgag taataatcct atccatacca gtgctatcag cataactgtg
    13801 cgaatgatga atcgcattac aacctcttct ctttttatgt tcgcttaatc gtagcggcaa
    13861 tatgcgctga agcaagctac tcattccgaa aaagcacgaa tatcgacata gttaggcgct
    13921 gtttaactaa cgcatgctag tttaatgaca taaggtaggt gaagcggaga ttggagtgaa
    13981 aaagtttcga tgggtcgttc tggttgtcgt ggtgttggct tgcttgctgc tttgggcgca
    14041 ggtattcaac atgatgtgcg atcaggatgt acaatttttc agcggaattt gtgccattaa
    14101 ccagtttatc ccgtggtgat atcatttttt cagcactaga ttttcttcct ctccagcagt
    14161 ggtagaatag cgcctctact cagaggtggt gaaatgaacg aagttgtaaa ttcaggcgtg
    14221 atgaacattg cgtctttggt tgtatcggtg gtggttattc ttatcgggct catcttgtgg
    14281 ttttttatca atcgtgccag ttcacggact aacgaacaga ttgaactgct tgaggcgttg
    14341 ctggatcagc aaaaacgtca aaatgcactg ttacgtcgtt tgtgcgaagc aaacgaacca
    14401 gagaaagcag ataaaaagac cattgagagt caaaaatcgg ttgatgacga agatattatt
    14461 cgcctggtcg ccgaacgata atccttcctc aatcttgagt aaccccgtcg ttgaagggtt
    14521 actcaattca tcctgattat acgcactgtc atggtgcaac tgcttcaaat taaaactctg
    14581 gtctgacgtc aaataattat catattattt atttgcgtaa cgggatatca gaaaaagata
    14641 ttttcctcat taatttatta ggatgtttac atcggatttg tgattaagcg tggtattatt
    14701 tattagcgaa acgtttctct cttgattttt ttgctcattc atcaattttt cttatttta
//
LOCUS       DAJTNA010000092        14693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTNA010000092 DAJTNA010000000
VERSION     DAJTNA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14693
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.093"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..1268
                     /gene="cmtA"
                     /locus_tag="OO161_002428"
     CDS             <1..1268
                     /gene="cmtA"
                     /locus_tag="OO161_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR5463468.1"
                     /translation="GWLPNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIG
                     TIGVIVGAEIPMFLGSMIMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLL
                     CLLGFEVIGPAVLIANTFVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYP
                     LGMQQASVNGKSIFFMVASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHEL
                     YFPYVLMKPLTIIAMIAGGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATI
                     AGVTVGTLVSFAITSLILKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAF
                     VCDAGMGSSAMGATTFRKRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVT
                     DKPLILINNYIGDPKLDTLFNQLTAEHKH"
     gene            1283..2560
                     /locus_tag="OO161_002429"
     CDS             1283..2560
                     /locus_tag="OO161_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5463469.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            2557..3522
                     /gene="yggF"
                     /locus_tag="OO161_002430"
     CDS             2557..3522
                     /gene="yggF"
                     /locus_tag="OO161_002430"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5463470.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            3544..4053
                     /gene="fumE"
                     /locus_tag="OO161_002431"
     CDS             3544..4053
                     /gene="fumE"
                     /locus_tag="OO161_002431"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5463471.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            4050..4763
                     /locus_tag="OO161_002432"
     CDS             4050..4763
                     /locus_tag="OO161_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5463472.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALREHLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(4735..5412)
                     /locus_tag="OO161_002433"
     CDS             complement(4735..5412)
                     /locus_tag="OO161_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5463473.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(5406..6083)
                     /locus_tag="OO161_002434"
     CDS             complement(5406..6083)
                     /locus_tag="OO161_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5463474.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHCTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6071..6778)
                     /locus_tag="OO161_002435"
     CDS             complement(6071..6778)
                     /locus_tag="OO161_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5463475.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(6779..7357)
                     /locus_tag="OO161_002436"
     CDS             complement(6779..7357)
                     /locus_tag="OO161_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5463476.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7380..7811)
                     /locus_tag="OO161_002437"
     CDS             complement(7380..7811)
                     /locus_tag="OO161_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5463477.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGKFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8183..9202
                     /gene="epd"
                     /locus_tag="OO161_002438"
     CDS             8183..9202
                     /gene="epd"
                     /locus_tag="OO161_002438"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5463478.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9252..10415
                     /gene="pgk"
                     /locus_tag="OO161_002439"
     CDS             9252..10415
                     /gene="pgk"
                     /locus_tag="OO161_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5463479.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            10630..11709
                     /gene="fbaA"
                     /locus_tag="OO161_002440"
     CDS             10630..11709
                     /gene="fbaA"
                     /locus_tag="OO161_002440"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5463480.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12067..12927
                     /gene="mscS"
                     /locus_tag="OO161_002441"
     CDS             12067..12927
                     /gene="mscS"
                     /locus_tag="OO161_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5463481.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13066..13701
                     /gene="argO"
                     /locus_tag="OO161_002442"
     CDS             13066..13701
                     /gene="argO"
                     /locus_tag="OO161_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5463482.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            13794..14534
                     /locus_tag="OO161_002443"
     CDS             13794..14534
                     /locus_tag="OO161_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5463483.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
BASE COUNT         3561 a         3571 c         4029 g         3532 t
ORIGIN      
        1 ccggttggct gcctaacgaa catttcgcca aaattgtcgg cccgatgatt acctatttat
       61 tgcccgtgat gattggttct acaggtggtc atctggtcgg cggtaaacgc ggggcggtca
      121 tgggcggaat aggtactatt ggtgtgatcg ttggcgcaga gatcccgatg ttccttggct
      181 caatgatcat ggggccgctc ggtgggttgg tcataaaata tgtcgataag gcactggaaa
      241 aacgcatacc tgccggtttt gagatggtta tcaataactt ctcattaggt atcgcgggga
      301 tgctcctttg tctgctgggt tttgaagtga tcggcccggc ggtgttaatt gccaatactt
      361 tcgtcaaaga gtgtatcgag gcgctggtac atgcgggtta tctgcctctg ctgtcagtca
      421 tcaatgaacc ggcgaaagtg cttttcctta ataatgcgat cgatcagggc gtctattatc
      481 cgctgggaat gcaacaggct tcggttaacg gtaaatccat cttctttatg gtggcctcta
      541 acccaggtcc gggcctgggg ctgctgctgg cgtttacctt gtttggtaaa gggatgagta
      601 aacgttctgc gcccggggcg atgattattc acttcctcgg tgggatccac gaactgtatt
      661 tcccatatgt gctgatgaag ccgctgacta ttattgccat gattgcgggc ggtatgtctg
      721 gtacctggat gtttaactta ctggacggtg gtctggtggc tggcccaagt ccggggtcta
      781 tctttgctta cctggcactg acgccgaaag gctcgttcct ggcgacaatt gccggtgtta
      841 cggtaggtac cctggtgtcc tttgctatta cttcgctgat actgaagatg gaaaaaacgg
      901 tggaaacgga gagcgaagat gaatttgctc agtcagccaa tgcggttaag gcgatgaaac
      961 aagagggtgc attctcgtta agcagggtta agcgtatcgc ctttgtttgc gatgcgggga
     1021 tgggctccag tgcgatgggc gcgaccacct tccgtaaacg cctggaaaaa gcggggctgg
     1081 caatagaagt aaaacattac gcgatagaaa acgtgcctgc ggatgccgat atcgtcgtca
     1141 ctcacgccag tctggaaggg cgcgtgaaac gtgtgacgga taaaccgctg atattgatta
     1201 ataactatat tggcgatcca aaactcgaca ctttatttaa tcaattaacc gccgaacata
     1261 aacactgatt acagaggtaa aaatgaaaac caaagttgct gctatttatg gcaagcggga
     1321 tgtccgtctg cgcgaatttg aactgccaga aattaccgat aatgaactgt tagtgagtgt
     1381 aatttctgac agcgtctgtt tatcgacctg gaaagcggcg ttactcggta gtgaacataa
     1441 acgcgtaccc gacgatttag aaaatcatcc ggtcattacc gggcatgaat gtgccggggt
     1501 tattgtcgaa gtgggtaaaa atctcactgg caaatataaa aaaggccagc gttttgtatt
     1561 gcaaccggcg atggggttac caagcggata ttcagcaggc tacagctacg aatattttgg
     1621 cggtaacgcc acctatatga ttattcccga aatcgccatt aatttgggct gcgtattacc
     1681 gtatcacggc tcttattttg ctgcggcgtc gctggtagag cctatgtgct gcattattgg
     1741 tgcttatcat gccaattatc acaccacgca atatgtttat gagcatcgca tgggcgtcaa
     1801 acctggcggc aatattgcac tgctggcgtg tgcgggtccg atgggcattg gcgctatcga
     1861 ttacgccatt aacggtggca tacaaccgtc acgggtggtg gtggtcgata tcgacgacaa
     1921 acgcctggcg caggtgcaga agctgctgcc ggtggatctg gcggccagta aaggcattga
     1981 gctggtgtat gtgaatacca aagggatgag cgatccggtc cagacgctgc gggcgctgac
     2041 aggagatgtc gggttcgatg acatttttgt ttatgcggcg gtgcctgctg tcgttgagat
     2101 ggctgacgaa ttactggcgg aagatggctg tctgaacttc tttgccgggc cgacggataa
     2161 aaacttcaaa gtgccgttta atttctacaa cgtccattac aacagcacgc acgtagtcgg
     2221 tacatccggc ggttcaacgg acgacatgaa agaggcgatt gcgttaagcg ccacggggca
     2281 gttgcagccg tcctttatgg tcacgcatat cggtgggctg gatgcggtgc cagataccgt
     2341 gctcaatctg ccggatatcc ctggcggtaa aaaactcatt tataacggcg tgaccatgcc
     2401 gctcactgcc attgccgatt ttgccgaaaa aggcaaaacc gatccgctat ttaaagagtt
     2461 ggcgcggctg gttgaggaaa cgcacggcat ctggaatgaa caggccgaga aatatctgct
     2521 ggcacaattt ggcattgata tcggggaggc cgcgcaatga tgtccctggc gtggccatta
     2581 tttcgcgtta cggaacaggc agcgttggct gcctggccgc aaaccggatg tggcgacaaa
     2641 aataaaattg atggcctggc ggtcaccgcg atgcgccagg cattaaacga cgtcgctttt
     2701 cgtggacgag tggttatcgg cgaaggagaa attgaccatg cgccgatgct gtggattggc
     2761 gaagaggtgg gcaaaggcga tgggccagaa gtcgatatcg cggttgatcc cattgaaggc
     2821 acgcggatgg tggcgatggg gcagagcaat gcgctggcgg tcatggcttt cgcccctcgg
     2881 gatagcctgt tacatgcgcc cgatatgtat atgaaaaagc tggttgttaa tcgactggct
     2941 gccggggtga ttgatctgtc tttgcctctg gcggacaact tgcgtaacgt ggcgcgggcg
     3001 ttaggtaaac cgctggacaa gctgcgcatg gttacgctgg ataaaccgcg cctgagtgcc
     3061 gcgattgagg aagcaacaca gctgggcgtg aaggtttttg ccctgccgga tggcgatgtc
     3121 gccgccagcg tgctgacctg ctggcaggat aatccttacg atgtgatgta caccatcggc
     3181 ggtgcgccag aaggcgtgat ttctgcctgt gccgttaaag cattaggcgg ggatatgcag
     3241 gccgagctga ttgacttttg tcaggctaaa ggggattaca cggaaaatcg gcagattgca
     3301 gagcaggaga gcaagcgttg caaggcaatg ggcgttgacg ttaaccgtgt gtactcgctc
     3361 gatgaactgg tgagggggaa cgatatcctc tttagcgcta cgggcgtgac gggcggcgag
     3421 ttggtgaacg gtatccaaca gacggcgaat ggggtgcgga cgcagacatt actgatcggc
     3481 ggcgcggacc aaacgtgtaa tataatagac tccctgcatt gatggtgatc tatcggaaga
     3541 ccaatggcga agctgacaga agatgatgtg cttgagcaac tggatgcaca ggacaattta
     3601 ttttcattta tgaaaactgc gcattctatt ttgctccagg ggatacgcca gtttctgccg
     3661 tcgctgtttg tcgataacga tgaagagatc gtggaatatg cagtgaagcc gttactcgcc
     3721 caaagcggcc cgcttgacga tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa
     3781 atggataaat ggctgtacgc cgatatcacg catttttccc agttctggca ttacctgaac
     3841 gaacaggatg aaacgcccgg atttgccgat gacatgacct gggattttat cagcaacgtc
     3901 aatagcataa cccgcaatgc gatgctctat gacgcgttga aagcgatgaa gttcgccgat
     3961 ttcgccgtct ggtcagaggc gcgttttagc gggatggtca aaacggcgct gacgctggca
     4021 gtaacgacaa tcttaaagga attaacgccg tgaaaattga attaacggtg aatgggctga
     4081 agatacaggc acagtaccag aacgaagaga ttgaaaatgt acataaaccg ttgctgcgta
     4141 tgctggcggc cttacagacg gtaaatccgc agcggcgtac ggtggttttt ctttgcgctc
     4201 cgccggggac gggtaaatct accctaacca ccttctggga atatctcgcg caacaagacc
     4261 cggagctgcc cgctattcag acgctcccga tggatggttt tcaccattac aatagctggc
     4321 tggacgcgca tcaattgcgc cccttcaaag gcgcaccaga gacatttgac gttgcgaaac
     4381 tggcggaaaa tctgcgccag gtggtggaag gggattgtac gtggccgcag tacgatcgac
     4441 aaaagcatga tcctgttgaa gatgcgttgc acgttaccgc accactcgtc atcgtcgaag
     4501 gaaactggtt gttgctggac gacgagaagt ggtgccagct tgcgcaattt tgtgatttct
     4561 cgatctttat caacgcgcca gctactgcgt tgcgggagca tctggtgggg cgcaagctgg
     4621 cgggaggggt atcgctggca gatgcagaag ccttttatga ccgtaccgac gggccgaatg
     4681 ttcgccgggt gctggaagag agtctgcctg caaatttgac cttaatgatg accgctacgg
     4741 gtgaatatcg tctggtggat taacgttcct gattacacca tcttccagtc gcaccacacg
     4801 agagaaatga cgccgggtaa atgcggcgtc atgactgatt gctaccacgc tcgtgcctcg
     4861 ctggcggcac ttctccagcc agctttcaaa cacgctcaac caattttcgt caaaatcccg
     4921 actcggctcg tccagcagta atagtggcgg agagagtgct tccaggcagg cgacggcgac
     4981 catccgtcgc tgcgcactat gcagatccaa cgggtgcgag ttggcagtgt ctgctaactg
     5041 acaacattgc agggcagcat gggtacgttg agtgatttca tccgccgggc atttttgcag
     5101 tttcaaacca aatgcgatct cgtcagcgac ggtgctgtga aaaagttggt tttccgcttc
     5161 ctgaaacagt acgccaacct ttgccgcgcg ctggcggttt ttcaggtttt tcatcgcctg
     5221 ttgttgcagc ataaccgtac cggctgtggg cgtaagaaga cctgccatta ctcgtaacag
     5281 cgtcgatttc cctgcgccat tgtcaccggt cagcgccaac cattcacctt gtttgagttg
     5341 cagagaaatg tcgcaaagac aatccgttgc cgcgccaggc cagcgataag atatctgatt
     5401 taacgttaac atagtggctg gagcgctccg ttttgcaatt gccacgcttg ctggcagtag
     5461 ttcaggaaag gtgtgcaatg acgttcaaaa agaataatca gcgaaccacg ttcaaacgcc
     5521 cagtgttgta agcgctgcaa cagcatttcg ctggcctgcg gcgtcaggcg gctaaaggct
     5581 tcatcaagaa ttaagagctt tggctgcatc gcgatggcgc aggcgatgac cacgcgctgt
     5641 gtctctccac cggagagtgt tgccgggtga cgatggcgca acggctggca ctcggtcaga
     5701 gtcagggcgg catcaatccg cgccataatc tctgcttcag caagacacag attttctggc
     5761 ccaaaggcaa cttcttcttc cacgctaaag gtacagccgg agagctgcaa atagggcgat
     5821 tgctggacga gctggatggt ggcggattgt tcattaagcg ggagatgccc aatgggcgta
     5881 ccgaggagcg tccccgtgcc tgttatctct cctggcagaa agtccggata ccagcccgcc
     5941 atcagctgtg ccagcgtact tttgccactg ccgttgtcac caaagatggc gaccatcccc
     6001 ggtttgacat agtgaaagtc ataacagaac ggaggatgcg ttgagtgtgt cgggcaataa
     6061 cggaactgtt ctaacgtaac catatccacg ctccgaattc tgtcagcatc agcaagatca
     6121 tggtgtaacg cgctacgcgt tgtagggtgc tatcggctgg tgcccacaac gttgttcgtc
     6181 ggttgtggat ccgaaatgcc cgcatatcca gcgccgcacc acggacagcc agatcgttaa
     6241 gcgcattgtg tgttagcgga ataattaacg cgggcatcgc acgtaaacgc tgataccagc
     6301 cctcatccag cggcacgccg cgggcacgct gcgcttcatg aataatcgct aattgccgtt
     6361 ttaactgctc aacgaccaat aacggcccgg caaacagata agcgacgcct ggcggcaggc
     6421 gagaagcaaa cagagcgcga ataaaacatt gcacggggac gtactgcatc cacaactgtg
     6481 aggttgaaac aatcgccaga atccgcaacc agagcgtaat agcatgcgac caccgttctg
     6541 gcgagcgtgg cgtgccgctt agccattctg gtagccagcc accgtgtacc agccacagcc
     6601 cggctcccag cgagaacatc agccagacga catattttgc ccgccgccgc gtggccttta
     6661 gcgcaataag gcagaaaaag gtcgccgcgc tatagatcgg caaaatggtc tgcgcgggca
     6721 ggataagcgt ggtgcaggcc gccaatgccc acagtgttaa tgaggtaaat ggatgcattt
     6781 agcgcacggc agccattgcc ggaaaatggc gcgtggtgcg aatcggcagc tggcgcagta
     6841 aaagccagac aatcaccgct gtaagaattt tatcgaccag gttcgcgcct atcaccgtaa
     6901 tcgccaccga ctccaccagg ttttgcccca tcgagtgcat ccaggcaacg aacaaatcgg
     6961 ctccgctacc ggtgacaccg ccgaacagcg cagtacgcag cggtacagcg acgaccgtta
     7021 ccgcaagcgt aataatcacg ccgctgacaa cgacttttgg cagggtgcga aaccaacccg
     7081 ctcgcgccag ccaaccggcg accaggccga tgaccatcgc gacgggtgca aatgcggcgg
     7141 cgatcgggtc ggtcagcaat ccccagagta aattggtcag taagccggtc agcattccga
     7201 tcaccggacc aagcagtacc gcgctaataa gtgtgccgat ggagtcgaga aatataggta
     7261 atttgaccat gctggccagt tgcccgccga tcatattgat ggcgatagat ataacgatca
     7321 acaccagtgc ctgactggaa aagtggctac gcgccataaa aaaacctctt tgaaagtagt
     7381 tatttaacgg gggaaatatg caactcgtcg taaccagaaa gcgcgcaagg ttggcggcta
     7441 aacgccagtt cctcaaggct accgatcacc agttcaagcg tggtccgcac ggtacagccg
     7501 ggcatcatat gaccgccaag catcgtgccg tgtggatcgg aaacgcagag atgaagatgc
     7561 tcaccactct gttccagcgt gccgtttagt gagatcactt cgaatttacc gttcaggagc
     7621 gtggtgcctt cttgcctggc atagcgcaag gcgacgtcag tcaggctacc ggtacagcct
     7681 gcaatccagg cggcatgaag ttgatgctgc tgtgcaaaag cgtgtagctg ggaaaaaact
     7741 tcctgcccgg ggaggaggcg cagtgcgtaa aagcgtgcgg aggaagaata tgggtagggc
     7801 aatgtcatca ttggagtact ccgaaactcg gacgtattgc ggataatatc gacgctgcat
     7861 cagtcatatt ctgatataag tcagggtttg tgacggcaca acgaaaagat tttgctacgc
     7921 ttattcgtga ttctgcaaag tggaataaat tccacagggg cggggctgaa aacgtgacgt
     7981 aagtcagata attgctttct ttgactggac aatcattcct tttattccac gtttcgctta
     8041 tcctagctga agcgtttcag tcgattaaat gttcgacaat taaccaatca gtcgcagttt
     8101 gcgacaggta aggtttcccc ggacgatttg ctggattact ctgtctggca aatttgctca
     8161 gggaaaacct tgcaggagat ctatgaccgt acgcgtagcg ataaatggct tcggtcgcat
     8221 cgggcgtaat gtggttcgtg ctttgtatga atccggacgc cgggcggaaa ttaccgtggt
     8281 ggcaatcaac gaactggcgg atgctgcggg catggcgcat ttgttgaaat atgacaccag
     8341 ccatggccgt tttgcatggg aagtacgaca ggaacgcgat caactttttg ttggtgatga
     8401 cgccatccgc gtattgcatg aacgttcact gcaatcgctc ccctggcgtg aacttggcgt
     8461 tgatgtagtc ctcgactgca ccggcgtata tggctcccgc gagcatggcg aagcgcatat
     8521 tgccgccggg gccaaaaaag tgctcttttc acatcctggc agtaacgatc tcgacgcgac
     8581 cgttgtttac ggcgtcaatc aggatcaact tcgtgcggaa caccgcatcg tttctaacgc
     8641 ttcctgtacc acgaattgca taattcccgt catcaaattg ttagatgatg cgtacggtat
     8701 tgagtccggc actgtgacca caattcactc cgccatgcac gatcaacagg ttattgatgc
     8761 ataccatcct gacctgcgtc gcacccgggc agccagccag tcgatcattc cggtcgatac
     8821 taaactggcc gccggtatca cacgattttt tccgcaattt aacgatcgct ttgaagcgat
     8881 tgcggtacgt gtgccaacca taaatgtgac ggcaatcgat ttaagcgtga cggtgaagaa
     8941 acctgtaaaa gccaatgaag tcaacctgtt gctgcaaaaa gcagcacaag gtgcatttca
     9001 tggtatagtt gactatacgg aattgccgtt ggtctctgta gattttaacc acgatccgca
     9061 cagtgccatt gtcgatggca cccaaacccg ggtcagtggc gcacacctga tcaaaacgtt
     9121 ggtctggtgc gataacgaat ggggctttgc taaccgaatg ctcgacacga cgttagctat
     9181 ggctactgtt gctttcaggt aagacgcaag cagcgtctgc aaaactttta gaatcaacga
     9241 gaggattcac catgtctgta attaagatga ccgatctgga tcttgctggg aaacgtgtat
     9301 ttatccgtgc ggatctgaac gtaccagtaa aagacgggaa agtaaccagc gacgcgcgta
     9361 tccgtgcttc tctgccgacc atcgaactgg ccctgaaaca aggcgcaaaa gtgatggtaa
     9421 cttcccacct gggtcgtcct accgaaggcg agtacaacga agaattctct ctgctgccgg
     9481 ttgttaacta cctgaaagac aaactgtcta acccggttcg tctggttaaa gattacctcg
     9541 acggcgttga cgttgctgaa ggtgaactgg ttgttctgga aaacgttcgc ttcaacaaag
     9601 gcgagaagaa agacgacgaa accctgtcca aaaaatacgc tgcactgtgt gacgtgttcg
     9661 taatggacgc attcggtact gctcaccgcg cgcaggcttc tactcacggt atcggtaaat
     9721 tcgctgacgt tgcgtgcgca ggcccgctgc tggcagctga actggacgcg ctgggtaaag
     9781 cactgaaaga acctgctcgc ccgatggtgg ctatcgttgg tggttctaaa gtatctacca
     9841 aactgaccgt tttggactcc ctgtctaaaa tcgctgacca gctgattgtt ggtggtggta
     9901 tcgctaacac ctttatcgcg gcacaaggcc acgatgtggg taaatccctg tacgaagctg
     9961 acctggttga cgaagctaaa cgtctgctga ccacctgcaa catcccggtt ccgtctgatg
    10021 ttcgcgtagc aaccgagttc tctgaaactg caccggctac cctgaaatct gttaacgatg
    10081 tgaaagctga cgagcagatc ctggatatcg gtgatgcttc cgctcaggaa ctggctgaaa
    10141 tcctgaagaa tgcgaaaacc attctgtgga acggtccggt tggcgtgttc gaattcccga
    10201 acttccgcaa aggtactgaa atcgtggcta acgctatcgc agacagcgaa gcgttctcca
    10261 tcgctggcgg cggcgacact ctggcagcaa tcgacctgtt cggcattgct gacaaaatct
    10321 cctacatctc cactggcggc ggcgcattcc tcgaattcgt ggaaggtaaa gtactgcctg
    10381 cagtagcgat gctcgaagag cgcgctaaga agtaaaaaat cacagggcag ggaaacctgc
    10441 ccttgtttca gcgcgctttt agagcacgca ttttttcata ctctaaataa ttcgagttgc
    10501 aggaaggcga caagagagtg aatccccagg agcttacata agtaagtgac tggggtgagc
    10561 gaacgcagac gcagcacatg caacttgaag tatgatgagt ataaggcccg acgatacagg
    10621 acaagagaca tgtctaagat ttttgatttc gtaaaacctg gcgtaatcac tggtgatgac
    10681 gtacagaaag ttttccaggt agcaaaagaa aacaacttcg cactgccagc agtaaactgc
    10741 gtcggtactg actccatcaa cgccgtactg gaaactgctg ctaaagttaa agcgccggtt
    10801 atcgttcagt tctccaacgg tggtgcttcc tttatcgctg gtaaaggcgt gaaatctgac
    10861 gttccgcagg gtgctgctat cctgggcgcg atctctggtg cgcatcacgt tcaccagatg
    10921 gctgaacatt atggtgttcc ggttatcctg cacactgacc actgcgcgaa gaaactgctg
    10981 ccgtggatcg acggtctgct ggacgcgggt gaaaaacact tcgcagctac cggtaagccg
    11041 ctgttctctt ctcacatgat cgacctgtct gaagaatctc tgcaagagaa catcgaaatc
    11101 tgctctaaat acctggagcg tatgtccaaa atcggcatga ctctggaaat cgaactgggt
    11161 tgcaccggtg gtgaagaaga cggcgtggac aacagccaca tggacgcttc tgcactgtac
    11221 acccagccgg aagacgttga ttacgcatac accgagctga gcaaaatcag cccgcgtttc
    11281 accatcgctg cgtccttcgg taacgtacac ggtgtttaca agccgggtaa cgtggttctg
    11341 actccgacca tcctgcgtga ttctcaggaa tatgtttcta agaaacacaa cctgccgcac
    11401 aacagcctga gcttcgtatt ccacggtggt tccggttcta ctgctcagga aatcaaagac
    11461 tccgtaagct acggcgtagt gaaaatgaac atcgataccg atacccaatg ggcaacctgg
    11521 gaaggcgttc tgaactacta caaagcgaac gaagcttatc tgcagggtca gctgggtaac
    11581 ccgaaaggcg aagatcagcc gaacaagaaa tactacgatc cgcgcgtatg gctgcgtgcc
    11641 ggtcagactt cgatgatcgc tcgtctggaa aaagcattcc aggaactgaa cgcgatcgac
    11701 gttctgtaag atattccttt ctgcttatct caaggcccgc tctgcgggtc tttttttcgc
    11761 caaaagcaaa attgcctgat gcgctacgct tatcaggcct acacgtatcc tgcaatatat
    11821 tgaatttgca ggtttttgta ggccggataa ggcgttcacg ccgcatccgg catgacaaag
    11881 cgcacattga tatctgtctg agccccgctc tgcggggatt ttttcgccaa aagcaaggtg
    11941 attcagatga gaaatctgtg atctatttgg caaaattatg ctttattgtt tacccttgtc
    12001 agactgcccg tcataaggcg gcggagtgta tttctccatt ttgagtcagt tgaaaaggaa
    12061 tattgaatgg aagatttgaa tgttgtcgat agcataaacg gcgcgggaag ctggctggta
    12121 gctaaccagg cgctgctgct aagttatgca gtaaacatcg tggcggcact cgcgatcatc
    12181 atcgttggtt tgattatcgc gcggatgatt tccaacgcgg tgaatcgcct gatgatctcc
    12241 cgtaaaatcg atgccactgt tgctgatttt ctttctgcat tagtccgtta cggtattatc
    12301 gcctttacgc taatcgctgc cctgggacgc gtgggtgtac aaacagcgtc agtcattgct
    12361 gtactcggtg ccgcaggctt agctgttggt ctggctttgc aggggtcact ttctaacctg
    12421 gccgctggcg tgttacttgt catgttccga ccgttccgtg ccggagaata tgttgacctc
    12481 ggcggcgtag ccggtactgt gctgagtgtg cagattttct ccaccaccat gcgtactgca
    12541 gacggtaaaa ttatcgttat tccgaacggt aaaattattg ccggaaatat tattaacttc
    12601 tcccgcgagc cagttcgccg taacgaattt attattggcg tggcgtatga ttccgatatc
    12661 gatcaggtta agcagatcct gaccaatatt atccagtctg aagatcgcat tttgaaagat
    12721 cgcgaaatga ctgtgcgcct gaacgaactt ggtgcatcgt cgattaattt cgtggtccgc
    12781 gtctggagca acagcggcga tctgcaaaac gtgtactggg atgtgctgga gcgtattaaa
    12841 cgtgaatttg atgccgccgg tatcagcttc ccgtacccgc aaatggatgt gaactttaag
    12901 cgggtgaaag aagacaaagc tgcgtaatca acgctgaggc cagataatac tctctggcct
    12961 ctctcttatt agttattctg attgccaatt aatattatca atttccgcta ataacaatcc
    13021 cgcgatatag tctctgcatc agatacttaa ttcggaatat ccaacgtgtt ttcttattac
    13081 tttcaaggtc ttgctcttgg ggcagctatg attctgccgc tcggcccaca aaatgctttt
    13141 gtgatgaatc agggcattcg tcgtcagtac cacattatga ttgccttact ttgcgctatc
    13201 agcgatttgg tcctgatttg cgcggggatt tttggtggta gcgcgttatt gatgcagtcg
    13261 ccgtggttgc tggcgctggt cacctggggc ggcgtagtct tcttgctctg gtatggtttt
    13321 ggcgctttta aaacagcgat gagtagtaat attgagttag ctagtgccga agtcctgaag
    13381 caaggcagat ggaaaattat cgccaccatg ttggcagtga cctggctgaa tccgcatgtt
    13441 tacctggata cttttgttgt actgggcagc cttggcgggc agcttgacgt cgaaccaaaa
    13501 cgttggtttg cgctcgggac aattagcgcc tctttcctgt ggttctttgg tctggctatt
    13561 ctcgcagcct ggctggcacc gcgcctgcgc acggcaaaat cacagcgcat tatcaatctg
    13621 gtagtgggat gtgtcatgtg gtttatagcc ttgcagctgg cgagagacgg tattgctcat
    13681 gcacaagcct tgttcagtta ggcgctatct gatggaaaaa taaaacagag gcgctaagct
    13741 tgcctccaga ggtcctgaat tttgggcaag tcaattaaca cggagagact aacgtgaagt
    13801 tcaaagttat cgccctagcg gcattaatgg gtattagcgg gatggcagcg caggctaacg
    13861 aattgccgga tggaccgcat attgtcacct ccggtacggc aagtgtggat gcggtgccag
    13921 acattgccac tcttgcgatt gaagttaacg tggccgcgaa ggatgccgct acagccaaga
    13981 aacaggcaga tgagcgcgtc gcacaataca tttccttcct tgaactcaat cagatcgcga
    14041 aaaaagatat cagctcagcg aatttacgca cccagccaga ttatgattat caggatggta
    14101 aaagtatcct gaaaggctac cgcgctgtga gaacggtgga agtcacgctc cgtcagttag
    14161 acaaactgaa ttccttgctg gatggcgcgc tgaaggcggg tcttaacgaa attcgttcag
    14221 tgtcgctggg cgtggcgcag ccggatgcct ataaagacaa agcgcgtaag gcagcgattg
    14281 ataacgcgat tcatcaggcg caggaactgg cgaacggctt tcatcgtaaa ctggggccgg
    14341 tatatagcgt gcgctaccat gtttccaact atcagcccag cccaatggtg cggatgatga
    14401 aagccgatgc cgcgccggtg tctgcccagg aaacttacga gcaggccgct attcagtttg
    14461 atgatcaggt cgatgtggtc ttccagttag aacctgtgga tcaacaaccc gctaaaacac
    14521 ctgcagcaca ataatacttg tagcccgacg tattcgtcgg gctaatgcct ttacccgata
    14581 ttgcctcctt tttctgtaat tttctgtaaa accatgacgt aatcacctat ctttgctcgc
    14641 ggtttcatca aggataggct gactgaaacg gttaagcgga caaggatgcg ggg
//
LOCUS       DAJTNA010000093        14569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTNA010000093 DAJTNA010000000
VERSION     DAJTNA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14569
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.094"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..1436
                     /locus_tag="OO161_002444"
     CDS             <1..1436
                     /locus_tag="OO161_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5463484.1"
                     /translation="EGSLLLWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAAL
                     LLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASL
                     LRGEFDAASARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPW
                     LSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVR
                     AVPLFSLFALISLASLALYGWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVG
                     TLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAG
                     VLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALF
                     DHQQRIGELMPERRFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSG
                     VRWIWGGGLLMIAGALLSGWRGRKRDE"
     gene            1429..1812
                     /gene="nrfF"
                     /locus_tag="OO161_002445"
     CDS             1429..1812
                     /gene="nrfF"
                     /locus_tag="OO161_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5463485.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            1809..2405
                     /gene="nrfG"
                     /locus_tag="OO161_002446"
     CDS             1809..2405
                     /gene="nrfG"
                     /locus_tag="OO161_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5463486.1"
                     /translation="MKEPKIPVKMLTALTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYTALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            2747..4060
                     /gene="gltP"
                     /locus_tag="OO161_002447"
     CDS             2747..4060
                     /gene="gltP"
                     /locus_tag="OO161_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5463487.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLMHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(4251..4939)
                     /locus_tag="OO161_002448"
                     /pseudo
     CDS             complement(4251..4939)
                     /locus_tag="OO161_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(5033..7180)
                     /gene="fdhF"
                     /locus_tag="OO161_002449"
     CDS             complement(5033..7180)
                     /gene="fdhF"
                     /locus_tag="OO161_002449"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6761..6763),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5463488.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWIAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(7378..8844)
                     /gene="mdtP"
                     /locus_tag="OO161_002450"
     CDS             complement(7378..8844)
                     /gene="mdtP"
                     /locus_tag="OO161_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5463489.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLQEEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(8841..10892)
                     /gene="mdtO"
                     /locus_tag="OO161_002451"
     CDS             complement(8841..10892)
                     /gene="mdtO"
                     /locus_tag="OO161_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5463490.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSNWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(10892..11922)
                     /gene="mdtN"
                     /locus_tag="OO161_002452"
                     /pseudo
     CDS             complement(10892..11922)
                     /gene="mdtN"
                     /locus_tag="OO161_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
     gene            complement(11941..12216)
                     /locus_tag="OO161_002453"
     CDS             complement(11941..12216)
                     /locus_tag="OO161_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463491.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(12425..14410)
                     /gene="yjcS"
                     /locus_tag="OO161_002454"
     CDS             complement(12425..14410)
                     /gene="yjcS"
                     /locus_tag="OO161_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR5463492.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVRGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVISLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3247 a         3763 c         3939 g         3620 t
ORIGIN      
        1 atgaaggttc gctgctgctt tgggtgctgc tgctttccgc ctggagcgcg ctgtttgcct
       61 ggcattatcg gcagcaaacc gatccgctat ttccgctgac gctagccgtt ttatctctca
      121 tgctcgccgc actgctactg tttgtggtgc tgtggtccga tcccttcgtg cggatatttc
      181 caccagcaat cgaaggccgc gatctcaatc cgatgctgca acatcccggt cttatctttc
      241 atccaccgct gctttacctt ggctatggcg gtttgatggt agcggcgagc gtggcgctgg
      301 cgagtctact gcgcggcgag tttgatgcgg ccagcgcccg aatttgctgg cgctgggcgt
      361 tacctggctg gagtgcatta acggcgggga tcatcctcgg ttcctggtgg gcctactgcg
      421 aactcggctg gggcggctgg tggttctggg atccggtgga aaatgcctct ttattaccct
      481 ggctttctgc cactgcgctg ctgcacagtt tatccctgac acgccagcgg gggatttttc
      541 gccactggtc gctgttactg gcgatagtta ctctgatgct gtcgctgctg ggcaccttaa
      601 ttgtccgttc tggcattctg gtttcggttc atgcgttcgc gctggataac gtccgcgccg
      661 tgccgttgtt cagcctgttt gcactgatta gccttgcgtc tctggctctg tatggctggc
      721 gagcgcggga cggtggcccg gcggtgcgtt tttcggggtt atcgcgggaa atgttaatcc
      781 tcgctacgct gttgctgttt tgcgcagtgc tactgatcgt gctggtggga acgctttatc
      841 cgatgattta cggtctgctg ggctggggac gcctctccgt tggcgcgccg tattttaacc
      901 gcgcgacgtt accgtttggt ctgttgatgc tggtggtgat tgtgctggcg acgtttgtct
      961 ctggcaaacg cgtgcagctt ccggcgctgg tagctcatgc gggcgtgctg ttatttgccg
     1021 ctgggatcgt ggtttccagc gtcagccgtc aggagatcag cctgaattta cagccgggtc
     1081 agcaggtgac gctggcagga tacaccttcc gttttgagcg cctcgatctg caagccaaag
     1141 gcaattacac cagcgaaaaa gcgatagtgg cactgtttga ccatcagcaa cgcattggtg
     1201 aactgatgcc ggagcggcgt ttttacgaag cacgtcgtca gcaaatgatg gaaccgtcaa
     1261 ttcgctggaa cggcatccat gactggtatg cggtcatggg tgaaaaaacc ggagcggatc
     1321 gttacgcttt tcgcttgtat gtacaaagcg gtgtgcgctg gatctggggg ggaggattgt
     1381 tgatgattgc gggcgcattg ttaagcggat ggcgggggag gaagcgcgat gaataaagga
     1441 cttctcacgc tactgctgtt atttacctgt tttgcgcgcg ctcaggtcgt agacacctgg
     1501 caattcgcca atccgcaaca acagcaacag gcgttaaata ttgccagcca gttacgttgt
     1561 ccgcagtgcc agaatcaaaa tttactggaa tccaacgcgc cggtggcagt cagtatgcgc
     1621 catcaggttt acagcatggt ggcggagggg aagaacgaag tcgaaatcat tggctggatg
     1681 accgaacgct acggagattt tgttcgctat aacccaccgt taacgggtca gacgctggtg
     1741 ttgtgggcgc tgccagtggt gttgttactg ctgatggcac tgatcctctg gcgagtgagg
     1801 gcgaagcgat gaaagagccc aaaataccgg tgaaaatgct gacagcactc acgattttga
     1861 tggtatttct ctgtgtcggc agttatctgt taagtccaaa atggcaggcg gtacatgcgg
     1921 aatatcagcg tcagcgcgat ccgctacatc agtttgccag ccagcaaacc ccggaagcgc
     1981 agcttcaggc attgcaggat aaaatccgcg ctaatccaca aaacagcgaa caatgggcgt
     2041 tactgggcga gtactatctg tggcaaaacg attacagcaa ttcgctgctg gcgtaccgtc
     2101 aggcgttgca actacgtggt gagaacgctg aactgtatac ggcgctggcg acggtgcttt
     2161 attaccaggc cagccagcat atgaccgccc agactcgcgc aatgatcgac aaagccctcg
     2221 cgctggactc taacgaaatc accgctctga tgctgctggc ttccgatgcg tttatgcagg
     2281 cgaattacgc gcaagccatc gaactatggc aaaaagtgat ggatctcaac tcaccccgga
     2341 tcaaccggac acagctggtc gagtcgatta atatggcgaa attgttgcag cggagatcgg
     2401 attaatcatc tggtgatgaa gattatgtta ttgagctgca taaaaaataa tcgaatgaac
     2461 atatgccaaa aataatcact aatcagtatt attgcagatt aaacaaataa aaatctttcc
     2521 ataacaaatg gttattcatt aatcctgaca taaactcgcg ttattatgca ttaatgcagc
     2581 gaaaagctct gttgttaaag ggttgcgcaa cataccgcgc aaatgatact gatcataagc
     2641 gttaaaaaaa tctacaaacc aacgcaacac aattcatgcc ctggcagtat gtcacgttct
     2701 cgcgtttctg aacggggaac ggcgctccat tgaggaagtt attctgatga aaaatataaa
     2761 attcagcctg gcctggcaga ttctgtttgc tatggtgctg ggcattctcc tgggaagcta
     2821 cctgcactac catagcgaca gccgcgactg gctggtcgtc aatttgctct ctccggcggg
     2881 tgatatcttc atccatctga ttaaaatgat tgttgtgccg attgtgatct ccacgctggt
     2941 ggtgggtatc gcgggtgtgg gtgatgccaa acagctcggg cgtattggcg cgaaaaccat
     3001 tatttacttc gaggtgatca ccaccgtcgc catcattttg gggatcactc tggcgaacgt
     3061 cttccagccc ggtgccgggg tggatatgtc gcagctggcg accgtcgata tctcgaaata
     3121 tcagagcact acggaagcgg tacaaagcag ttcccacggc attatgggca cgattttgtc
     3181 gctggtgccg acgaacattg tggcgtcgat ggcgaaaggc gaaatgctgc cgatcatctt
     3241 cttctcggtg ctgtttggtc tggggctttc ctccctgccc gcgacgcatc gtgaaccgct
     3301 ggtgaccgtg ttccgctcca tctctgaaac catgtttaaa gtgactcaca tggtgatgcg
     3361 ttatgcaccg gtgggtgtgt ttgcgctgat tgcggtgacg gtggctaact ttggtttctc
     3421 ttctctgtgg ccactggcga aactggtgct gctgatgcat ttcgccattc tgttcttcgc
     3481 gctggtagtg ctgggaattg tggcgcgcct gtgcgggtta agcgtctgga tcctgattcg
     3541 tattctgaaa gatgagctga ttctggcgta ctccactgcc agctctgaaa gcgtactgcc
     3601 gcgaattatt gagaagatgg aagcctacgg agcaccggcg tcgatcacca gcttcgtggt
     3661 gccgaccggt tactctttta accttgatgg ttcgacgctg tatcaaagta ttgccgctat
     3721 tttcatcgct cagctgtatg gcattgacct gtccatctgg caggaaatca ttctggtgct
     3781 gacgctgatg gtgacctcga aagggattgc tggcgtgccg ggcgtgtcgt ttgtggtgtt
     3841 gctggcaacg ctgggtagcg taggtattcc gctggaaggt ctggcgttta ttgctggtgt
     3901 tgaccgtatc ctcgacatgg cgcgtactgc gctgaacgtg gtgggtaatg cgctggcggt
     3961 gctggtgatt gccaagtggg aacacaaatt tgaccgcaaa aaggcgctgg cttatgagcg
     4021 tgaagtgctg ggcaaatttg ataaaactgc ggatcaataa ttgaagattg ccggggttat
     4081 ccacccggca atgtgtggat gcctgatgcg acgctggtgc gtcttatcag gcctacgcca
     4141 gacagcgcaa tagcctgatt tagcgtgatt ttgtaggtcg gataaggcgt ttacgccgca
     4201 tccgacatca acgcctgatg cgacgcttaa cgcgtcttat caggcctacc ttaaccgttg
     4261 gttaattttt caaactcttc acacccggta tcaaaccctt ccatacagct caggttcaac
     4321 cagtgcagcg ccttttgttt gttcttctcg ataaaacctt cttcaccgtt taagaacatc
     4381 attcccgccc agtactcgga ataaccggtt cgggaaattg cagagctgcg tttgaaatac
     4441 caggttgcct tgtcatcgtc ggccttaatg cccacgccgt tggcgtaaat caaaccaagc
     4501 agcatttggg catcgacggc agagtcgttc tccagatctt ccgaggcttt tctaacagcg
     4561 aaatcgcctt tggataatcc ggtttacccg cttgagtatt taccagaata tgcgccagcg
     4621 ttacttcacc ttctttactg cccgcttgcg ccgctttctc tgcaagaact tttgcttgtg
     4681 gatagtccag actgaccgga ttggtgattt tgatctgcgc caacagcgcg caggcatcag
     4741 catcaccgct gtcggtggct ttctgtgccc aatattcggc tttgctcaaa tcgccggagc
     4801 taaaccagct gtcggcaaga aaatattgtg cgcgtcggtc cccggcctct gctgccttta
     4861 aatactggct gccaggctcg gagtcgttgg cgtgggcaaa gaatgtcaaa aacaacatca
     4921 atgcgataat ttttttcatt ttgataatac gattgggtag ggagtaacca gtataaagag
     4981 ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta gaaaggactg tattacgcca
     5041 gtgccgcttc gcgcaggcga gttttcaact tgttgtactc gtcaatcacg tactgctcgg
     5101 cggcgcgctg atcggcgatc ggctcgacgc gcacggcgca gtatttgtat tccggcgttt
     5161 tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca ggcaccaatc caccactggt
     5221 aggtcatgta aatcgcccct ttgttcggac gatcgctgac ctgcgcacgg gtgataattt
     5281 tgcctttacg cgagtgcacc caaaccaatg cctcatcttc aatacccaga cgtttggcgt
     5341 cttcggtatt gatttgtgcg tagccaggtt catcagccag cgccgccagt gccgcacagt
     5401 taccggtcat cgaacggcaa gagtagtgac caacttcacg caccgttgac agtaccatcg
     5461 ggtactcgtc ggtgagtttg tcgattggcg ctatccagtc gcaggtgaag aactgcgcca
     5521 gaccgttcgg ggtatcaaac ttctctttaa acagataaga agtcccctga tcggcatctg
     5581 aagtatcgcg gcaaggccac tgaatgaagc cctgttcgcc cattttctcg taagtcgcac
     5641 cgtagaaatc cgggcacaga tgacgcaact catcccagat ctcctgggtg ttgttgtagt
     5701 gcatcggata acccatacgg gtggcgattt cactgatgat ttgccagtcc gttttcagat
     5761 cccatttcgg ttcaactgct ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca
     5821 cgccttcatg ctcgccccac gacgttgacg gcaaaatgac atccgccgcc gacgcggttt
     5881 tggtcataaa gatgtcctga acgatgacca gttccagatc ttcaaaggct ttacgtactg
     5941 ccgacagctc cgcatcagtt tgcagcggat cttcgcccat aatgtacgcg gcacgcactt
     6001 cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata gccggtatga gcaggcaggc
     6061 tttccacgcc ccaggctttg gcgaatttct cgcggttagc cggatctttc acgtactggt
     6121 atcccggata cgtatccggc agcgcgccca tatcgcaggc accctgcacg ttgttctgac
     6181 cacgaaccgg gtttacaccc gcatgcggct taccgaggtt accggtcagc atcgcgaggc
     6241 tggtcagaga acgcacggtt tccacgccct ggtagaactg ggttacaccc atgccccaca
     6301 ggatggcggc gcttttcgcc tgggcataca tccgtgccgc ctgacgaatc tcactggcgc
     6361 tgacgccggt gatatcttca accgactccg gcgtgtagcc ttcaacgatt ttacgatact
     6421 cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc gtacagattt tcttcaataa
     6481 tgacatggcc catcgcattc aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca
     6541 tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca gacgataatt ttcgccccgt
     6601 tacgtttagc gttaattacg tgattcgcca cgattgggtg ggaatccgcc gggttgtacc
     6661 cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat agcattgctc attgcgccat
     6721 taccgaccga ttggtgcaga cctgcaaccg atgggccgtg tcagacacga gcgcagcagt
     6781 caacgttatt ggtaccaata acggcgcgcg caaatttttg cattacatag ttggtttcgt
     6841 tacccgtacc acgcgaggag ccggtcgtct ggatggcatc cggaccgtac ttctctttga
     6901 tggcgctcag gcgctcggca acgtaattca gtgcctcatc ccaggaaaca ggttcgagtt
     6961 tgccgccacg ctggcgacgg atcatggggg ttttcaggcg cggggtcagg atctgggtat
     7021 cgttaatgaa gtcccagcca taataaccct tcagacacag ggtaccctgg ttggttttcc
     7081 cctgcgctgc ctccgcccgg acgattttgc cgttatcgac gaccaggttg attttgcaac
     7141 ctgatgcgca ataggggcaa accgtgacga cttttttcat cggtctcgct ccagttaatc
     7201 aaatcacgca tacgcgctct cgactacagt acgcatcttt tatgccacat tttatttggg
     7261 gtcattccct gatattacgg gcactattta ttcaaaactc tgacgaaaaa caggctgtcg
     7321 tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc atcacgcggc agacatttta
     7381 ttttttctcg acgacgggag ccgcctgata cccgccgccc agcgatttca tcaactgaat
     7441 gctgtggatc acccggcggc tgtccagcat cagtaatgac atctcttcgg caagcactgg
     7501 caaccgggct tcggtggcct gtaagcggct ggttaagccg cgctgatagg cggcctcggc
     7561 agcgcgctgg gtgaagcgcg ttgcttccac gcgttcagcc tgcatttctc gttcgtcgtt
     7621 gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc accgcgttca gtactgactg
     7681 gttgtaacgt tcaatcatca tgttgctggc ggcgcgagtg ccttcgagat tggcattcaa
     7741 ccgtccaccg tcaaacagcg gcaatttcag acccgggatg aagttgaact ggcgactggt
     7801 ttttttgaat aaggtatcca ggtggatgga gtccagaccg aaaaacgctt tgatatcaaa
     7861 gctcgggtag aacagtgccc gcgcggaatc cacctgatct aatgacgcct gaacatacca
     7921 gcgcatggct tgcagatccg ggcgtctggc gagcaactca taagagagtg tcgccggaat
     7981 gccggtctgg actcgtggta atgccaccgg tttgatctcc ggcatatcgc tggcccccgc
     8041 gccaatcaat gcacgcagag attctcgcgt ttcggtgatt tgccctttga cggcagcaat
     8101 ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg aaaggcactt gtgcttccag
     8161 accgtgcgcc actttactct ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg
     8221 ttctaacaga tcgagcatct gatagctggc ctgcatactg taataaagct gcgctacgcc
     8281 cgtggtcagc gatagctcta ctgctgcggt ttctgccagc gcggcattat gcgcgccaat
     8341 ggcggcggca accgctgagc gatgcacacc ccacaaatca agatccagtc cggcaaacaa
     8401 acctactgtg gcttccgtat agtacggccc gtccataccc agtgagggcg catccatcgc
     8461 ataagggctt aaaaagccgt tcgccgagac gcgttggcgg ttgagcatcc ctaacgctgc
     8521 gacctgtaat tgtgaaccgg catctaacaa atcggcctgg gactgcgctt tttcttcctg
     8581 cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc cgttggatca gcgcatccag
     8641 ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc cagccggagc tggcaagatg
     8701 aatatcgtcg gccagtttga tttgttccgg tttgagctgt tgatgaggtg cagaatcctt
     8761 acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg agaatgctgc acaacagcag
     8821 acgtgaaagt tgacgattga tcattgcgtg gctccttgtg cctgttccgt ggcgggcgtt
     8881 aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt
     8941 tttaataagg tgggcaggat ggcctgcgag gcgggtgtct cttcaagcgt tatttgagga
     9001 gaacagctga gtgcggttgc cagaccggca gcgtattttt ccagggcgtc ggcaaactga
     9061 cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg ccgagttcca ggtggcatcc
     9121 caggcgtgaa gaagatcgcg gccctgacga ataaccgttt gcgaacgttc aatcagtaag
     9181 gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc gttggcacat ctcttcacag
     9241 gcattaaacg ccgcatgcag acctatacga atttgcagat aagtgcgcag agccgtgact
     9301 tcctgctggc gtgggatccg cattacttta cttagcatac ccagcgcacc ggctagtttt
     9361 tgcggcagag tgcgcgcttc actttcaggc cagacaaagg tgtaaatcac cgcggacacc
     9421 acggtaccaa tgatgatacc cagggcgcga tcgcgaattt ccaccaggtc gtacactggg
     9481 ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacga ccatctgtgt gccgatataa
     9541 gaagagcgtt cagagctggt ggcaatccat gcgcccaaca ggaaaatcgg tgccagcaca
     9601 aacagcaatt cgacaatatt gtccagccag ggcatgacca gtagcgtgaa taacagcgcc
     9661 agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca tcttctggta cgacgaaccg
     9721 acatttggat tagcgacgat cacgcaggtc agcatacagg tgtgaatgcc ttcccagtcc
     9781 acgccgctgt aaaaggtgta acagatcaaa caggcgagca atgtttttac cgcgtagcgt
     9841 atatagtctg gattggtaaa agcatcggcg accattgatg gcggtttggc ggcgggcgtt
     9901 ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg tctggcagat attctccagg
     9961 ttacattccc gtgccgccac cgcttcactt tcactgatcc gccagttgct ttgccagcac
    10021 tgaccttcag caacggcatg ctgcagcttg ttgatttctg aggctaattt ttgccgaaat
    10081 tcaataattg cctgagaatc agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa
    10141 atgtaggtta ccgttgccac gcagctttgc caccatgcgc tttgagttcg ccagttggca
    10201 tcgtccgcga gacaaaagac attcagtttt tgcagcgcca gcgcctctct ttcaatccgc
    10261 gtttcgggta gcggtgcgag gctgtctgtc aggtgactaa tggcatcatc aagccgatca
    10321 ttaagcgcct gatgcatttg cgaaatggca cgactgggaa accacagcac gccgattaac
    10381 gtcatcagca aggttggata gaggccaaca acgatacacc acagcgttaa gcgcacgacc
    10441 acttccggat agtcgagcat ggcggggaag gtttgcccgt aaatggcgac aatggcgaca
    10501 gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa acatgcagcc catcaggatc
    10561 ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg accatttgta gatcaaaaac
    10621 aggctgccga tctccagcac cgtggcaacc acaaacaaga tcgccacaaa tttggtgtaa
    10681 aacgcgttcg actgaatacc gtaaaacagc actgccagcg ataacgccac aaaagggatc
    10741 tcaaaagtca tcgagatcag aatcaccagc aggcagccta cccagagttg cacgatctgc
    10801 ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa agaacgccag cagcctgacc
    10861 accggtaatg gcagggagtt gagcgcgctc attattgcgg ctcaagatta gcgactgccg
    10921 aagcgccgat gcggaacatt tccgggtcag gtttatcgac catgattttg accgaaaaac
    10981 gctgggcaac gcggacccag ttaatagagc gagaaacttt cggcaggccg cccagcacca
    11041 ggccgccgtc attcggtagc acgccgtagc caatcgaatc cactttaccc tcgaaggttt
    11101 tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc tgagcgaata tttttcagat
    11161 cggtttcgcg gaagttggcg atcacgtacc agtgacgagt gtcgattagg gtaaaaatag
    11221 ggcgcatggc agaagcaaat tgcccgacgg aggttttgag ggaaatgacc cggccatcaa
    11281 acggcgcgcg aacggtcgcc atttccagat gcagtttggt cagggcaata tccgcttcga
    11341 ccgccgcacg ctgggcaact aatacatcca cgccgctgac ggcgctggcg gctgactgcg
    11401 cctgtaacaa tacggcatta agatccgctt ctgcggcgcg ctgcgccgtt cttgcacggt
    11461 caacatcttc cgctgagaca aaaccttctt tcagtaatgg ctcggtgcgg cgtaatgtat
    11521 ctgtggcctg tttcgcggcg gcacgtgctt tttctaccgt ggcattaacc gagtcggcac
    11581 caaactgttg ggcgtcaacg ctacgctggg tgagcataat ttgcttatcc agcgccgcga
    11641 gggaggcttc agctttcgcc agattggctt cgtacgggcg cgggtcaatg cggaacagca
    11701 aatcgccctg tttgactgcc tggttgtcgg tgaccccagt tctacaatgc ggccgctgac
    11761 ttccggcacc acatcaatgg tatctgctga cgcgtaagcg tcattagttg atggcgcact
    11821 gtctacgcgc cagataacga aaacaagggc aaccagcgcc aacgcaacca ctaacagagc
    11881 agggaattta ctgcgaggag cttttttcgg cgtactttcc atgagcgcgt ctcaaataga
    11941 ttagaaaaat gccagccaga acagcatggc gtagacagca aaaagggcgg tataaataat
    12001 tccgacaaat gccagattga tatttgcccg ctgaataatt cgcctcgtga ttagcgtcaa
    12061 aataaggctg gcaatggcgc agaaaaacca gctgggataa taagcgccga tcaccgggat
    12121 tgccggagat aatgagcatc ccgaaaccat gaagacggga attatccagg ctgttttttt
    12181 gagttgcatc gccatgcgag agagaactgt tggcatatta aatacatcct gtttgctgcg
    12241 aacaatatct gtagtgaata tcaatatcca ttgaaggcgt cagtatggct attttcttga
    12301 tgaataaaaa tagctttatt cagccaccgc attcataact tatatatttt atgtattaat
    12361 tgattgtatg tatttttata ataaatgctt ttgggggctt aagcgccccc aaaagtgagt
    12421 ggcattaatt tggggttacg atattcaccc acaaatcgaa attatccaga caggcaataa
    12481 tttcttccag tttcgcgcca ttgccaataa ttttggcttt cttcgctttt accagatccg
    12541 ccattttggc ttgtcctgtc agcacggcgt gcagatcttc acggctgatg tagaaagagg
    12601 cgtcggcttg cggttgcagt gttttacggt agttaagcac gctatcgttc agcgtcaggt
    12661 tgaggttatc gccgttgctc atattgaagt tcaggctgat atttttaccc gcagctttcg
    12721 cgctatcgag gcgaacggac ataaagtcga acagcatttc gaccgacatc ccgcgaatgg
    12781 tgtccgggga accggtggtg ccgtggctga acttatgcac cccttcgcgc agctctttcg
    12841 cgccggtcag gtagaaaccg cgccaggtgg cggactcggc ctgatagccc agctgttcaa
    12901 agttattcgc ttgcagattc ttcgcgacct ggtcacccgg gttggcggca atcacctgtt
    12961 tcagcagttc tgccgaccag cggtaatcac cttgcttgtt cgcttcttgc gccaggctga
    13021 tgacacgggc agaaccgccc agcgcctgca cgtaacgttt acccatctcc acctgaccat
    13081 acggatgcag gttagccggg ttaccgtcgt aatagccaag atagaagtta tacaccgcgc
    13141 gggcgttgtg gctgacagaa ccgtaatagc cacggctggc ccagttattg gcaagtgcag
    13201 gcggcagttt aatcatgtcg ccgatttcat tcatggtgta gccctggttc gccaggtgca
    13261 gggtctggtc gtgaatgtac ttgatagtat cgcgatattt accaatataa tcattgatat
    13321 gcttattgcc ccggaccggc caggtgtgcg gcataaacag gacttccgcg tcgttacccc
    13381 acatatccag cgtttcgttc agatactcgg tccacttgct ggtgtcgcgg gttttcgcgc
    13441 cgcgcagagt gtagaagttg tgcagggtat gcgtggcgtt ctcggcggta cacagcgctt
    13501 tcagggccgg aatatagaag tgcatttcgg ctggtgcttc gctacctggg gtcatcagga
    13561 agtcaaactc caggccgtcg ataatcatct tctcaccggt tttggtgatg gttttgttcg
    13621 gtgcgatgat gctcgggtag cccgatgcca gcgttacgcc aagaccattg ccgatgttac
    13681 cctgagggtt atgcgccagc agcaggccgt aggagtaaag tgcacggcgg ctcataatat
    13741 tacccgccag tacgttttcg ctgatggctt cgtccataaa gccagcaggg gcgataacct
    13801 gcactttacc ggatttcaca tcagcttcag agataatgcc tttcacgccg ccatagtggt
    13861 cggtatggct gtgggtataa ataaccgcaa caatcggttt ttgcgggcga ttttggaagt
    13921 aaaggtcaag ggcggctttt gcgctcggtg gcgtcaccag cgggtcgatg acaataatcc
    13981 cggtatcacc ttcgataaag gtgatgttgg agatatcctg accgcgaacc tggtacattc
    14041 tgtcggtgac tttaaacagg ccggaaatac cgtttaactg cgactgacgc cacaggctag
    14101 ggttaacggt ttcgggcgcg ggcgcattga tatcaaattt ataatcttcc ccgcgataat
    14161 atggtttgcc attagcatcg cgcagaatac cttcatccag cagcggagcg ataaatccac
    14221 gctgagcgtc gtcaaaatcc tgacgatcgc taaacggtaa tgatttggcg taattctgat
    14281 taacctgttg ggtatattga gtggcggctt tcgcttcctc tttcgcgtta gcggtattta
    14341 ccatcatgcc gctggcggca gttaacgcaa taacaattct gctcaaacgg aataaccgag
    14401 agttattcat ataaatctcc atttatcatt tatgtcgtaa atatgtaatc catacaaaat
    14461 acgtcattta aagctggggt tatcataatt gggaatttgc gactttaata agtggaagtg
    14521 tgagctgaac gatccatttt attaggcatt tgtgctgaag agagaaaat
//
LOCUS       DAJTNA010000094        14394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTNA010000094 DAJTNA010000000
VERSION     DAJTNA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14394
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.095"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..126)
                     /locus_tag="OO161_002455"
     CDS             complement(<1..126)
                     /locus_tag="OO161_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR5463493.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRD"
     gene            complement(139..1428)
                     /gene="arsB"
                     /locus_tag="OO161_002456"
     CDS             complement(139..1428)
                     /gene="arsB"
                     /locus_tag="OO161_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5463494.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1483..1836)
                     /gene="arsR"
                     /locus_tag="OO161_002457"
     CDS             complement(1483..1836)
                     /gene="arsR"
                     /locus_tag="OO161_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5463495.1"
                     /translation="MSFLLPIQLFKILDDETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            2575..2658
                     /locus_tag="OO161_002458"
     CDS             2575..2658
                     /locus_tag="OO161_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5463496.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(2712..4064)
                     /gene="gorA"
                     /locus_tag="OO161_002459"
     CDS             complement(2712..4064)
                     /gene="gorA"
                     /locus_tag="OO161_002459"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5463497.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4136..4978)
                     /gene="rlmJ"
                     /locus_tag="OO161_002460"
     CDS             complement(4136..4978)
                     /gene="rlmJ"
                     /locus_tag="OO161_002460"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5463498.1"
                     /translation="MLSYRHSFHTGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMLHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5181..7223
                     /gene="prlC"
                     /locus_tag="OO161_002461"
     CDS             5181..7223
                     /gene="prlC"
                     /locus_tag="OO161_002461"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5463499.1"
                     /translation="MMNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7231..7983
                     /gene="rsmJ"
                     /locus_tag="OO161_002462"
     CDS             7231..7983
                     /gene="rsmJ"
                     /locus_tag="OO161_002462"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5463500.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTTLTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(8032..9501)
                     /gene="dtpB"
                     /locus_tag="OO161_002463"
     CDS             complement(8032..9501)
                     /gene="dtpB"
                     /locus_tag="OO161_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR5463501.1"
                     /translation="MNTTTPMGMLQQPRPFFMTFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(9819..10253)
                     /gene="uspA"
                     /locus_tag="OO161_002464"
     CDS             complement(9819..10253)
                     /gene="uspA"
                     /locus_tag="OO161_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5463502.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            10644..10979
                     /gene="uspB"
                     /locus_tag="OO161_002465"
     CDS             10644..10979
                     /gene="uspB"
                     /locus_tag="OO161_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5463503.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11223..12722)
                     /gene="pitA"
                     /locus_tag="OO161_002466"
     CDS             complement(11223..12722)
                     /gene="pitA"
                     /locus_tag="OO161_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5463504.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            12954..14156
                     /locus_tag="OO161_002467"
     CDS             12954..14156
                     /locus_tag="OO161_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5463505.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
BASE COUNT         3608 a         3688 c         3811 g         3287 t
ORIGIN      
        1 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
       61 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      121 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
      181 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
      241 agtcatattc ttctgcgaaa gtacgtgcag ccagagtagt gtagccaggc taccaattgg
      301 ggtaattttc ggtcccaaat cgcagccaat cacattggca taaaccatcg cttctttgat
      361 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
      421 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
      481 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
      541 caggccataa accaccagat acatgccgag cgagaagatg acaatttgcc agggggcacc
      601 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
      661 cgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga cgaaaaatcc
      721 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
      781 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
      841 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
      901 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
      961 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     1021 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     1081 aagcgctgcg ccatcattgg caaacagggc ggcaacggca gcaccgagca ggacaatcca
     1141 ggtaaacagc aagcgaccac gaccattacc ccagcgtgag acgtgcagcg ccgcccattc
     1201 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcgataa acgcagccgt
     1261 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatggacca cgcccgtaac
     1321 taacgccagt actgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     1381 gataaccaat acaatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcctgg
     1441 ataaaacaca tgcgaaaata catatgtgtt tagctaaatt ttttaactgc aaatgttctt
     1501 actgtctgcg gaacagtttt gtcgagccag gttgcggaca attgcctgaa tcttttcctg
     1561 ttcacatcgc caggcttcat caattatttt cgccgcccat gctggaatat gcggcgataa
     1621 gcggtaatga acccatttac cttgcttgcg gtccagcaat agcccgcttt cacgcagcaa
     1681 tgccaggtgg cgggagatct tgggctgcga ctggtcgaga gcagtgcaga gatcgcagac
     1741 gcataactct cccagttcgc tgagcagtaa aacgatgccc agacgggttt catcatcaag
     1801 aattttgaac aattggatgg gtaacagaaa tgacatattg cgctcctgat tgttgcaggt
     1861 agtgtctctc ttcgaagcgg ataagtcaaa acatatatga tttaacgaat atgtaagtgc
     1921 agaggaaggt aataggtgtg aattttgagt tggctattca tttgaaagga ggtctgaatc
     1981 aggacgtaaa aaaaccgact ttgcgtcggt tttttacttt ccagccctga gttggtggct
     2041 ctggctggag tgagagagtt cttatctaac agctcaatac tattagccag tggctaacat
     2101 tgcaagaatt agcgatttct tcagctggcg gagggagcac ttcggtatat atcgcagtga
     2161 cgatttatat actttcactg ggtcatcgtc atattaagcc ttgtccgaat ccggatgtaa
     2221 aaaaaccgac tttgcgtcgg ttttttactt tccagccctg agttggtggc tctgactgga
     2281 gtgagatagc caccatctaa cgcatcaatg ctaataccat actgaaacta ttgcaaggac
     2341 gtgctggttt tataacctgc atgtactgta tgattatcca gttagctctg aggcattttc
     2401 actctggcaa tgcgcataaa cgctttcaaa gtcctggtca gaagtacggg tggtgccgtt
     2461 aactgatgct ctggccggag tgagagagtt cttatctaac aatgagacat gcgccgtgac
     2521 aggcagtgga tgagtaagcg gatgcattct cactccatcg catggagaaa acgggtgatt
     2581 gataaagcaa tcatcgttct aggggcgtta attgcgctgc tggaactgat ccgctttctg
     2641 cttcagcttc tgaactgata gcggaaacgt aattaagggc taagagcaca ctactcttag
     2701 ccctttaaca tttaacgcat tgtcacgaac tcttctgccg ccgttgggtg aatggcgacg
     2761 gtattgtcga agtctttttt ggttgccccc atcttcagcg ccaccgcgaa gccctgcaac
     2821 atttcgtcca taccaaagcc aatgccgtga ataccgacaa tcttctcttc cgatccaacg
     2881 cacaccagct tcatgcggca cggctggcgg tgagtggtga cggcggtata catcgcggtg
     2941 aaagaggatt tatacacttt cacctgatcg tcgccatact gctcgcgcgc ctgcggttcc
     3001 gttaaaccaa cagtaccaat cggcggatgg ctgaagacca cggtcggaat gttgctgtaa
     3061 tccagatgct catccggctt gttattaaac aggcgttcag agagacggcg acccgctgca
     3121 actgccaccg gtgtcagctc cactgcaccc gtgttatcgc ccaccgcgta aataccttca
     3181 atgttggtgt tttgatattt atcgacgacg atatagcctt tttcgttggt tttaacgcca
     3241 gcggcttcga ggttgatgtt gtcattggca ggctcgcgac caatcgccca aatcaggcaa
     3301 tccaccgttt cactgcgacc atcttccagc tccagcgtca ggctaccatc ggcatttttc
     3361 actaccgctt tcgggatggc gttggtgtgc agctgcgggc cttcggcgtt catcacttcg
     3421 accagcgttt cggaaatcat cgggtcgaag ctgcgcagcg gcgcatgttt acgcacaaac
     3481 agatgcgttt tcgcgccgag gccgttaatc acgcccgcca gctcaacggc gatgtaaccc
     3541 gcgccaacaa ccgccacgcg ctctggcaaa gcaggaaggg cgaagaagcc atcagaatca
     3601 ataccgtatt ccacgcccgg aatatccggg tggctcggac gaccgcctgt ggcgatcaga
     3661 atatgatcgg ccgtgatggt ttcgccgttt acctccagca ttttggcatc aacgaagcgg
     3721 gcaaagcctt tgattacatc aacgttattt ttaccgagca cgttttcata ggaagtatga
     3781 atacggtcga tataggcggt acggctggcg atcaacgttt cccagttgaa cttgttgatg
     3841 gtggtatcga aaccataatc agggccgtac atatggatcg cttcacggat ttgcgccgcg
     3901 tgccacatca cttttttcgg cacgcagccg acgttcacgc aggtgccgcc cagctctttg
     3961 gcttcaatca gtgcgcattt ctggccgtac atagccgcgc ggttgatgga ggctataccg
     4021 ccgctgccgc cgccgatggc gatgtaatca taatgtttag tcatgacaga gtgtccttat
     4081 cgttgattac cgcgattgta gcgcgagacg taataggtgc cagcaatggc tgcaattact
     4141 ccggcacgat ccagcttacg gtggcgtgcc cggtgcctgc cggaaccagt ttgctgtgca
     4201 gccacggcag cacgttattc atctgctgtt ccagtttcca cggcgggtta atcacaatca
     4261 tgccggaagc ggtcatgcca cggcgatcgc tgtctggcag taccgccagt tcaatttgca
     4321 gaattttgcg aataccggtc gcttccagat cgtggagcat gcgcttaatt tgctgacgca
     4381 gcaccaccgg ataccacagt gcgtaagtac cagtggcgaa acgtttgtaa ccttctgcta
     4441 tcccgctgac caccgcctga tagtcggttt tcatttcata cggcgggtcg ataaggatta
     4501 aaccacggcg ggaaaccggc ggcagtttgg ccttaagctg ctggaaacca tcggcttttt
     4561 cgacgcgcgc acggctgtct ttctgaaatt cagaacgcag caacgggtaa tcgctcgggt
     4621 gcagttcggt cagttgcagg ctgtcttgtt cgcgcagtag ctggcgagca atcaatggcg
     4681 aaccggggta gtaacgcaac tgaccgctac ggttgaagtg ttttaccaca ttgatgtacg
     4741 cctccagttc tgcgggcaaa tcgtcctgct gccagatacg ggcgatgcct tcgagatatt
     4801 cgccggtacg ctcggcatgt tcgctgccta actgataacg cccggcccct gcgtgggtgt
     4861 cgagatagag aaacggttta tctttctctt tcagcgactc gatgatcagg ctctgaacgg
     4921 tatgtttaag gacgtcggcg tggttgccag tgtgaaagct gtggcgataa ctgagcatgg
     4981 gtaaaggtgt tccggtaagt aaaaatcggc ccgtattcgg ggcgcacaaa agcgtatcag
     5041 gacagtatac cgaaaagagg ccgccgccgc gaaagcgtaa cgtttctcat tgaaattcac
     5101 tacacttaac cccatgctac acacattatg taaagcgcct gttgagcgct tccttaacct
     5161 ctttaaccag gactgcgcga atgatgaatc cgttactgac tccctttgaa ttgcctccgt
     5221 tttctaaaat tctcccggaa catgtcgttc cagccgtgac tcaggcgctg aacgattgcc
     5281 gcgaaaatgt ggagcgcgta gtagcgcaag gggcaccgta cacctgggaa aatctctgcc
     5341 agccgttggc ggaagtggac gatgtgttgg ggcgtatctt ctccccggtc agccacctga
     5401 actcggtgaa aaatagcccg gaactgcgtg aagcctacga gcaaaccctg ccgctgctgt
     5461 cggaatacag cacctgggta gggcaacatg aagggctgta taaagcgtac cgcgacctgc
     5521 gcgatggcga tcattacgcc acgctgaaca cggcgcagaa gaaagcggtt gataacgccc
     5581 tgcgtgattt tgaactctct ggcataggtc tgccgaaaga gaaacagcag cgttacggcg
     5641 aaattgcgac ccgtctttct gaactgggca accagtacag caacaacgtc ctcgatgcga
     5701 caatgggctg gaccaaactc gttaccgacg aagcggagct ggcggggatg cctgaaagcg
     5761 cgctggctgc ggcaaaagcc caggccgaag cgaaagagct ggaaggctac ctgctgacgc
     5821 tggatatccc aagctacctg ccggtaatga cctactgcga caaccaggcc ttgcgtgaag
     5881 agatgtatcg cgcttacagc acccgcgcct ccgatcaagg cccgaacgcc ggtaaatggg
     5941 ataacagcaa ggtgatggaa gagatcctcg ctctgcgtca cgaactggcg caactgctgg
     6001 gctttgaaaa ctacgccttt aaatcccttg ccactaaaat ggcagaaaac ccgcagcagg
     6061 tgctggattt cttaaccgat ctggcaaaac gcgcgcgtcc gcaaggcgaa aaagagctgg
     6121 cacaactgcg cgccttcgcc aaagcggaat ttggcgtcga tgagttgcag ccgtgggata
     6181 tcgcgtacta cagcgaaaaa caaaaacagc acctctacag catcagtgac gaacagctgc
     6241 gtccgtactt cccggaaaac aaagcggtta acggcctgtt tgaagtggtg aaacgtattt
     6301 acggcatcac cgctaaagag cgtaaagatg ttgatgtctg gcatccggat gtacgtttct
     6361 tcgaactgta tgacgagaac aacgaactgc gcggcagctt ctacctcgat ctgtatgccc
     6421 gtgaaaacaa gcgcggcggg gcgtggatgg atgactgcgt aggccagatg cgtaaagctg
     6481 acggttctct gcaaaaaccg gtcgcgtatt tgacttgtaa cttcaaccgc ccggtaaatg
     6541 gtaaaccggc actgttcact cacgacgaag tgatcaccct gttccacgag ttcggtcacg
     6601 gcctgcacca tatgctgacc cgcatcgaaa ccgctggtgt ttccggtatc agcggtgtgc
     6661 cgtgggatgc ggtcgaactg ccgagtcagt ttatggaaaa ctggtgctgg gagccggagg
     6721 cgctggcgtt tatctctggt cactatgaaa ccggcgaacc gctgccgaaa gagttgctgg
     6781 ataaaatgct ggcggcgaag aactaccagg cggcgctgtt tattctgcgc cagctggagt
     6841 tcggcctgtt cgatttccgc ctccatgccg agttccgccc ggatcagggt gcgaaaatcc
     6901 tcgaaactct ggcagaaatc aagaaactgg ttgccgtagt accgtctcca tcctggggcc
     6961 gtttcccgca cgctttcagc catattttcg ccggtggtta tgccgcaggt tactacagct
     7021 atctgtgggc tgacgtactg gcggcagatg ccttctcgcg ctttgaggaa gagggcattt
     7081 tcaaccgtga aaccgggcag tcgttcctcg acaacattct gagccgtggc ggttcagaag
     7141 agccgatgga tctgttcaaa cgcttccgtg gtcgtgaacc gcagctggat gcgatgctgg
     7201 agcattacgg cattaagggc tgatcattca gtgaaaatct gcttaattga tgaaacaggc
     7261 gccggagacg gcgccttatc tgttctggcg gcccgctggg ggctggagca cgatgaagac
     7321 aacctgatgg cgctggtgtt aacgccggaa catctggaat tgcgcaagcg tgatgagcca
     7381 aaacttggcg gcatctttgt tgattttgtt ggcggagcga tggcgcaccg acgcaaattc
     7441 ggcggtggtc gcggtgaggc ggtggcgaaa gcggtgggca ttaaaggcga ttatttgccg
     7501 gatgtggtgg acgccaccgc agggctgggg cgcgatgcct ttgtactggc ttcagtcggc
     7561 tgccgcgtgc ggatgctgga gcgtaatcca gtggttgccg cgctactcga cgacggcctg
     7621 gcgcgtggtt atgcggatgc ggaaatcggc ggctggttgc aggagcggtt gcagttaatt
     7681 cacgcctcca gcctgacgac gctaaccgat attaccccac gcccgcaggt ggtctatctc
     7741 gatccgatgt tcccgcataa gcagaaaagc gcgctagtga aaaaagagat gcgtgtcttt
     7801 cagtcgctgg tgggaccgga tcttgatgcc gatggattac tggagcctgc acgcttgctg
     7861 gcgaccaaac gcgtggtggt aaagcgcccg gactacgcgc caccgctagc gaatgttacc
     7921 acgccaaacg cggttgtgac taaagggcat cgctttgata tctatgcagg tacgcccgtg
     7981 taaaagtaaa gcagggtagc gataacgcta ccctgctttc tgcaagaata attaatggct
     8041 ttccggcgtc gcaatcatgc gtttcagcca cggcaccatc aacagcatca ctactgcaac
     8101 gcccagcgtg accagaccaa tcttaccaaa cacgttggta tagacgggca acgtctcaag
     8161 cggatcggta atgttgtccg gcaccgcggt aaatgttgcc acatagccgc ccagcaagaa
     8221 cgcggcagcc tgcgtcagga accacatccc gagaataaag cccatcaaat gctgcggcac
     8281 cagggcagca atcatcgcca ggccaagggc gctaataaac agttcaccta agctctggaa
     8341 taagtacacc agcacgataa accatggcga tgtcagccct tgtgcatccg caaaccacat
     8401 tcccgcagct gccgccgtca aaaagcccag tgagcacata aacatgccga gagtaaattt
     8461 catcggcatc gagaggtctt tgcctttgtt acccagatgc gtgtaaatgc ctgccagtat
     8521 tgggctggcg agtaccaccc agaacgggtt aagcgcctgg aagctgaccg ggttgatgga
     8581 aaaaccgaga atttcatgat gcacgttgtt gatggcaaag aagttcagcg atgttggcat
     8641 ctgggcgtag agaatgtaaa acaccaccgc ttcgagcatc aggacaaagg cgacaaacat
     8701 tttattgcgc ccggttttat ccagcttgaa tgcctgacga aagaagatga tggtgacgac
     8761 gatggagaga acaatcagca ccagattggc gacttctacg ttgtgcatca gccatgcaca
     8821 tacgaagatc atcaccacgc tgccaagtaa tacgtacaac agtttgctga agctcatcgg
     8881 cttgaagtcg ggttcagaac caatgtcttt caccattcca cgacaggcga tgtaaaccag
     8941 taatgcgata attaaccccg ccccgcacag gttgtaggtg actgaataac cgaatctatc
     9001 agcgatcaca ggggccagcg ataacgctat caacgagccg atattgatcg acatatagaa
     9061 cagggtgaat gcgccatcaa gccgcggatc tttcggcgga tagcacttcg aaagcaagct
     9121 ggctgggtta gctttaaaca ggccgttacc gacagcgata gtccccaggg cgatgaaaat
     9181 caggtcaggc ttaagtagcg acatgccggt catgaagtag ccaatcgcca gcacaagtgc
     9241 tccgagaaca atggtgcgtt tggtccccag caggtggtcg ccgacatagc cgccaatgga
     9301 aatgaggcca tagaccagcg cagcaaaagc accaaaagtg acaaaagcct gctcttgcga
     9361 gaatccaagc tgtttaacga agaaaaccgc cagtacgccc tgcacgccgt agtagccgaa
     9421 tcgctcccat aactcgacaa aaaaggtcat gaaaaatggg cgaggttgct gcagcatccc
     9481 catgggtgtt gttgtattca tatttattaa ccttccaata ccatcctgaa aagtatgaac
     9541 gcgtaaggga taacgcgcga aaaagtttac atctgtcaga gattcttaat attgatgcgg
     9601 tgaatagatt aatacactat tacaacagaa aataaccagc taatcacact aatgatggaa
     9661 aatatgatgg tgttaaatgt tgtattggca ggagattttg attgcgtacg cagatcgtag
     9721 gccagataag gggtttacgc tgcatcaggt aacaaggcgc gccaaaaagc aaaacgcccg
     9781 catatgcggg cgttcaggaa cacgtcgtgg agggaagatt attcttcttc gtcgcgcagt
     9841 ggaacaatca gcatatcaac gtgaacggtg ttgatcagct gacgtgcgga agacatcagt
     9901 ttgctccaga agtcctggtg gtgaccacaa accaccagat ccatatcgta tttcttgatt
     9961 gcatcgacca gaacctggcc caggtcgccg ctgccgctca gggtttcagt gattgggtag
    10021 cctgcattag tggaaagctc ggtcagcgca tgatgtgtct cttcagagat gcgtttctgc
    10081 atatcaccca gattcacatc aataagcccg gtgtataggt cagagtagtt tacatctacg
    10141 tggatcagag aaactttcgc attgtagggg cgagccatag agactgcttt ctctaccaga
    10201 actttgcttt ccggggagag atcgaccgcg atgagaatgt gtttataagc catagtgtta
    10261 ctccttccat aaagttgtcg atgactggcc agctagcgtt tcttgtgccg ttacgtcacc
    10321 cgcgtcctgc gaactacgtg cgcggggcta acccagccgt aaagattcag tgttaagacc
    10381 atccttacct tatagcgacc cggatgatcc gtcaatccgc cttgcttacc cgttagataa
    10441 acaaattttc gacatgaata tattgatagt ggttaacctt ctggaaaaaa aacaacctga
    10501 tctcctacac tatctataga gccgctcgga tgttggtcac cacaaaagcg gtttctccag
    10561 gacctggtgt taactgattg gcagcgtatc gggagcggta gccccggggt ggaactccgc
    10621 gggcaggtcg ccggggagga ggtatgataa gcaccgtcgc attattttgg gctttatgtg
    10681 tcgtttgcat tgttaacatg gcgcgctatt tctcatcact acgcgcgttg ttagtggtac
    10741 tgcgtaactg cgatccattg ctctatcaat atgttgatgg agggggcttt tttacctcac
    10801 atggccaacc caacaaacag gtgcgtctcg tttggtatat ctatgcccaa cgttatcgcg
    10861 atcatcacga tgatgagttt attcgccgct gtgagcgggt gcgtcggcag tttattctga
    10921 ctagcgcatt gtgtggtctg gtggtggtca gcctgattgc attgatgatt tggcattaag
    10981 cacttcagca ataataacgc ataaaaaaag cgggccagta aactgacccg ctcgagactg
    11041 attacaaacg caaaattgcc tgatgcgcta cgcttatcag gcctgcataa tctctgcaat
    11101 ctattgaatt tgcatgtttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
    11161 caaagcgcac tatgtcacaa tctgaagcgg gccagctgac ccgctcgttt tggtgcgtac
    11221 gattacagga actgcaagga gagccagtac agcccgccgg aaagcagtac cgcagccgga
    11281 agggtaaaca cccaggccat cagaatgctg gtcacggttt tacgctgtaa gccgccacca
    11341 tctaccacca tcgtccccgc gacagaagag gagagtacgt gagtagtgga aaccggcatc
    11401 ccggtataac tcgccaggcc gatagacact gccgccgtca tctgggcaga catcccctga
    11461 gcgtaggtca tgcctttctt accgattttc tcaccgatag tcgttgccac acgacgccag
    11521 ccaatcatcg taccgatacc taacgccagc gcgaccgcca tgatgatcca caccggtgca
    11581 tactcgatgg tgctaagcat gtcggacttc agtttcttca acaggcgctg atcgtcagca
    11641 ctcacgccag gtatcttcac tactttgtcg atagtgtcag aaacgcacag cataatgcgg
    11701 cgcatctggc tacgttgatc aagcgacagc ttgtcgtagc tttccacatc ggtagttaac
    11761 atacctttca ggcggttgag cgcgttaatg gtattcgacg gatggcaatg gaactccgcg
    11821 ggttgcgttg cgccagcttc cggagccggt actaactgat cagcaccggt agcctgtttc
    11881 agcagcgcag gatgctgctc aaagtaagct tcgacgttgt tgatggcatc acgggtacgg
    11941 gtgatttcgt agccagtggc attcatattc accacgaagc ctgctggtgc gacaccaatc
    12001 aataccaaca taaccagacc aatgcctttc tgaccatcgt tcgcgccgtg cgaaaacgcc
    12061 acgccgatag cggaaaggat cagcgcaata cgcgtccaga acggcggctt tttcttgccg
    12121 tctttctttt cacgctccgc tggggtcagg tggatacggg cgcgtttctt ggtgccgctc
    12181 cagtagcgac gcagcaagaa aatcagaccg ccagcaaaca ccaggccgac aataggggaa
    12241 acgatcagag aaccgaaaat acttaatact ttcgggatat tgagtgcatc caccactgac
    12301 gtcccggtca tcaacgcatt ggttaaacca atcccgatga tcgcgccaat cagtgtatga
    12361 gagctggatg caggtaaacc aaagtaccag gtacccaggt tccagataat cgccgccagc
    12421 aacatagaga acaccatggc aaggccatga gacgatccca tattaagcag cagatccgtc
    12481 ggcagcatat gcacaatggc ataggcaaca ctcagaccac ccagcaaaac acccaaaaag
    12541 ttgaacaccg ccgccataac cacggcgagc tgagaacgca tcgcgcgggt atagataacg
    12601 gttgccacgg cgttggctgt gtcatggaaa ccattgatgg cttcgtagaa cagcacaaaa
    12661 gccagtgcaa gcaataataa cagcccggta tgcaaatcca ggccagcaaa caaatgtagc
    12721 ataggacgtt acgccatttt gaggacatga acgcggcgca ttatcagtga ctttcgcggc
    12781 gcgggcaaag tgaaatatag actttttttg atttgcctcc tgtatggatt tcactcaaaa
    12841 aataattatc ttatataatt caggcaaata cttcctttta gtaatattga tgctggtgtg
    12901 accactgagg aatctttaca attcacgccc gttttttcta agaggagcgc aacgtggaaa
    12961 ggtttgatgc cattattata ggcgctggtg cggcgggtat gttctgttct gcgctggcag
    13021 gtcaggcagg acgccgggtt ctgctgatcg ataatggtaa aaaaccaggg cgcaaaatcc
    13081 ttatgtctgg cggtgggcgc tgcaacttta ccaaccttta tgtcgaacca ggcgcttatc
    13141 tgagccagaa tccgcatttt tgtaagtctg cactcgcgcg ttttacccag tgggatttca
    13201 ttgatctggt caataaacac ggcatcgcct ggcacgagaa aacgttagga caactcttct
    13261 gcgatgactc cgcgcagcag attgtcgaca tgctggtgga tgagtgcgag aagggcaatg
    13321 taaccttcag attgcgtagc gaagtgctga gtgtggcgaa ggatgaaaca ggcttcacgc
    13381 ttgaactgaa cggcatgact gtcggttgcg aaaagctggt catcgcgacc ggtgggctgt
    13441 caatgccggg gctgggcgca tcgccgtttg gttataagat tgccgaacaa tttggcctca
    13501 acgtgctgcc gacccgtgcg ggtctggtgc cattcactct gcataaaccg ttgctcgaag
    13561 agttacaggt gctggcgggc gtggcggtgc cttccgtgat taccgctgaa aacggcaccg
    13621 ttttccgtga gaacttactc ttcacccacc gcggcttatc tggaccggcg gtgttgcaga
    13681 tttccagcta ctggcaaccg ggtgaatttg tcagtatcaa tctgctaccg gatgtggacc
    13741 tcgaaacctt cctgaatgag cagcgtaacg cacatccgaa tcagagcctg aaaaacacac
    13801 tggcggttca tctaccgaag cggttggttg aacgcttaca gcaactcggg caaatcccgg
    13861 atgtttcgct aaaacagctc aacgtgcgtg accaacaggc actgattagc acattgaccg
    13921 actggcgcgt acaacccaac ggcactgaag gctatcgcac tgccgaagtg acgctcggcg
    13981 gcgtggacac caacgaactc tcttcacgga cgatggaagc gcgcaaagtg cctgggctgt
    14041 acttcatcgg cgaagtgatg gacgttaccg gctggctggg gggctataac ttccagtggg
    14101 cgtggtcgag tgcgtgggct tgtgcgcagg atttgattgc agcgaagtcg tcctgacctg
    14161 ttacctaaag atgaaaatta ttagagccgc attaaaaatg agctaatttt gatagtggct
    14221 atcttgtgat tattttctaa tgagcccgtg aactgaaacc ctccaagctt aatataaggt
    14281 ggaaggaaag atgattgaaa actcacccag tggccagatc tttataaaat atgaacaggc
    14341 tgaaaaagac agtaactttg ttgttttttt atccttaacc actatgcatt aatg
//
LOCUS       DAJTNA010000095        14325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTNA010000095 DAJTNA010000000
VERSION     DAJTNA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14325
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.096"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..161
                     /locus_tag="OO161_002468"
     CDS             <1..161
                     /locus_tag="OO161_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5463506.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="OO161_002469"
     CDS             complement(199..1260)
                     /locus_tag="OO161_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5463507.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMSVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="OO161_002470"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="OO161_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5463508.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="OO161_002471"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="OO161_002471"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5463509.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4200)
                     /gene="hda"
                     /locus_tag="OO161_002472"
     CDS             complement(3454..4200)
                     /gene="hda"
                     /locus_tag="OO161_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5463510.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="OO161_002473"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="OO161_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5463511.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="OO161_002474"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="OO161_002474"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5463512.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="OO161_002475"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="OO161_002475"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5463513.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTTSAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="OO161_002476"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="OO161_002476"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5463514.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="OO161_002477"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="OO161_002477"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5463515.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLSKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIREHDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="OO161_002478"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="OO161_002478"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5463516.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12123..14315)
                     /gene="pdeF"
                     /locus_tag="OO161_002479"
     CDS             complement(12123..14315)
                     /gene="pdeF"
                     /locus_tag="OO161_002479"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5463517.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPFLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQVLLVGNLIGVPLCYFIIRVVRNPFDLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPFEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKP"
BASE COUNT         3774 a         3673 c         3579 g         3299 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggtc agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc gatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatggc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actagcaggt tgccgtccag cgcctgaata atcagataca ccgcgaagca
      421 gctccagaat tccgtgcctg cgccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag aaccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccag gtggcgatcc ccaccacgct
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggcaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta acgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gattaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgaca aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaaaaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacga tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctag
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacaacg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctaatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acctcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgaccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctca atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagcctgca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aaccgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaaa ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgcg gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc ctcttctgcg tccagcacca cttcttcagg gcgcagcaca ctgatcagtt
     4321 tgaaaatcaa acttaagccg ataccgacga tggtcgccag cgccatacct tttaactccg
     4381 ccgcgccgat gtttaccttc gcgccactga cgccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt tcgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccgc
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtggagcca aagaagccgg agatcacggt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtagcccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac gaaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctga
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag cgaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcataccaa gacccgcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacgatttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga tcattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgca aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccac ttcagcggtg atcagcggca tcgcggaagg
     6961 ttgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgaa gattacgatg tcgcgggttt ctgcgtgggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgttctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccacg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg caggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta ttgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctcaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggtaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccgt acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggcgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacgtcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgcccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcat ctgtaaatcg caagctccag cagttttttt ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gagcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctattgctg gagatggcgc gcaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggcttc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaggatgatt
     8881 acacctatct ggcggtggaa attatccgtg gagacaccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcgccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gctgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc cacatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacacgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgcgtggc gaaagattca cgcatcatcg actcgatgat ccacgccgca cataacggta
     9601 agaaagtcac tgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc agtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gcggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcacctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gcacgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttacgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgagtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgtggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga gcgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgc aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctacggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 aggtggcaaa tcgatgccac tatctgataa tccagactat ttgacacaat attgcggata
    12301 aaactgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaaaagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggctga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catgccatcc
    13021 caaaagaaac gaaattgctt gagatggcta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggggcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 acatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagagaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa atcaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aagacctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaaaaa ggcagaaaca gcggaagccc ccaccattta
    14281 tcacgtattt ttatataagt tgcattcagt ttcatagatg ctcag
//
LOCUS       DAJTNA010000096        14273 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTNA010000096 DAJTNA010000000
VERSION     DAJTNA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14273
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.097"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(64..1257)
                     /gene="lplT"
                     /locus_tag="OO161_002480"
     CDS             complement(64..1257)
                     /gene="lplT"
                     /locus_tag="OO161_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5463518.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLIALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(1250..3409)
                     /gene="aas"
                     /locus_tag="OO161_002481"
     CDS             complement(1250..3409)
                     /gene="aas"
                     /locus_tag="OO161_002481"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5463519.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            3798..4829
                     /gene="galR"
                     /locus_tag="OO161_002482"
     CDS             3798..4829
                     /gene="galR"
                     /locus_tag="OO161_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5463520.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(4836..6098)
                     /gene="lysA"
                     /locus_tag="OO161_002483"
     CDS             complement(4836..6098)
                     /gene="lysA"
                     /locus_tag="OO161_002483"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5463521.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGPVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            6220..7155
                     /gene="lysR"
                     /locus_tag="OO161_002484"
     CDS             6220..7155
                     /gene="lysR"
                     /locus_tag="OO161_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5463522.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(7142..7834)
                     /gene="ygeA"
                     /locus_tag="OO161_002485"
     CDS             complement(7142..7834)
                     /gene="ygeA"
                     /locus_tag="OO161_002485"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5463523.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(7963..9381)
                     /gene="araE"
                     /locus_tag="OO161_002486"
     CDS             complement(7963..9381)
                     /gene="araE"
                     /locus_tag="OO161_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5463524.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSHLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMITTLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGI"
     gene            complement(9696..10457)
                     /gene="kduD"
                     /locus_tag="OO161_002487"
     CDS             complement(9696..10457)
                     /gene="kduD"
                     /locus_tag="OO161_002487"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5463525.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(10487..11323)
                     /gene="kduI"
                     /locus_tag="OO161_002488"
     CDS             complement(10487..11323)
                     /gene="kduI"
                     /locus_tag="OO161_002488"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5463526.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVRYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(11610..12791)
                     /locus_tag="OO161_002489"
     CDS             complement(11610..12791)
                     /locus_tag="OO161_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5463527.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            13046..>14273
                     /locus_tag="OO161_002490"
     CDS             13046..>14273
                     /locus_tag="OO161_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5463528.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNPLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         3693 a         3847 c         3345 g         3388 t
ORIGIN      
        1 tgcatcaggt ttatacttat ccggcaccat aagattaacg ccggttaaaa ccggcgttaa
       61 atattaatga cggcgctgcc agatccacag cgccgttatt gccagcgcaa acagcgcacc
      121 gaagccaatg ccaatgggca cgaccgggat gcctaccatt accgccagcg agtaaatgcc
      181 cagcatcaac aacatggcgc tgttttcgcc aaggttttgt actgcaatcg cattccccgc
      241 cccgacgctt tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc
      301 ccccatcacg ccaatcagca tcaacaaggc ataggctggc agcagctcgt gttgcaggga
      361 aaaaatcagt accaccacgc caatcaaaat cccggctggc atacagcgtg acacggtttc
      421 cagcgtaact aacttcgctg ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc
      481 gttgagatag gtgggcgtag cgttatcggt aatgcccagc gccaccggta cccacagcac
      541 caacaggaaa cgcagcgtga cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa
      601 acgcgtttca ccattgcgcc atagcgaggt gcaggcattc aggaaactgc gggtcatgtt
      661 gatgagattc caggactgcc ccggacgcgc cgccgccagt ttgggaatgt agatattggc
      721 aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg gcgatgagga catgccagtc
      781 agccagcaca ccaccggcta cggaaccgag caaaatcgcc gctatggtag aagcttccat
      841 taaaccgtta gctttcacta acttactacc cgtggttaat tcgccgagaa taccgtattt
      901 cgccggtgaa taggctgcag caccaacccc caccagcgta tagccgagaa acggattgat
      961 accaaagcag atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg
     1021 gcctttggcg aagctatccg ccacctgccc gacaaacggc gcaaaaagaa tgtaagcacc
     1081 tacaaacacc atttgcagga tgggctggct ccactccgga tagaactgcg ctttcagtaa
     1141 cgccagagtg gcaaacagta gggcattatc gccaaacgca gagagaaact gcgccacgat
     1201 aaccgctttc atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt
     1261 gttgttccgc ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa
     1321 gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt
     1381 gctcgcgggc atactgttgc aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca
     1441 ccagtgcctc gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg
     1501 aaacaccaag tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg
     1561 caaagcgttt tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat
     1621 cgccagtgtc ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt
     1681 ccagtacacc tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt
     1741 gcaggcgtcc gccctcttcg ataccaggaa ccgacaacag gcgcgcatcc attcctggta
     1801 gaatacgccc taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg
     1861 cgcactcggt tacaccatag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct
     1921 gtttggtact ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt
     1981 catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg aacaacacgg
     2041 tgcaactgcg gtcatacacc aactccggca caatgcggta atgcagcggg cttggataaa
     2101 ggaacacttc tgcacctgta agcagtggcg taaacaggcc taccgtcagc ccaaaggagt
     2161 gaaacagcgg taatgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa
     2221 tctgctcgac gttcgccaga atgcttttat ggctatggac gacgcctttt ggatggcctt
     2281 cagaaccgga ggtaaagagg atcagcgcct cttcttccgg ctgctgtttt acctgcgcca
     2341 ggcgcggcat cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt
     2401 ttaaatcttc cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt
     2461 tgcctttatc gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaatag
     2521 cactggtcag cccttttacc ccagcagtgt agttcatcat tgcgggaatg cggcgacgcg
     2581 cgatggcccc aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc
     2641 gttcgccttc aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca
     2701 atttgcgata ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga
     2761 agcggtacat tgcactcagt aaagattcgt acagagtttc acgcgggcgc accgccatac
     2821 gcgcttccat cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac
     2881 gcgacgcatc cggcatcgcc acctgcgttg gaggcaaaat atgcagagta atttgcggga
     2941 acaagcgacg tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa
     3001 tacgcacagg aataaccgtt gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga
     3061 ttttcatcag cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc actggtcgac
     3121 cctgttccac cagacgtacc agatgtttaa tagccatagg ttgcgtcggg tcgagaggaa
     3181 caaagtcgat aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa
     3241 cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga
     3301 cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc
     3361 gaacgcgata caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct
     3421 ttgctctgat tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga
     3481 cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg
     3541 tacgaagagc gtaccgaata atctcaccaa tcaatacctc tgggattacc actttagcaa
     3601 cctgaagcca aacgccacca gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag
     3661 gtttgcaatc tctttttttg tcttacttat ttgcgtttgg ctcacattcc cacgatgaaa
     3721 acacgccccc ccttgaacca acgggcgttt tccgtaacac tgaaagaatg taagcgttta
     3781 cccactaagg tattttcatg gcgaccataa aggatgtagc ccgactggca ggcgtttcag
     3841 tcgccaccgt ttcccgcgtc attaataatt cacccaaagc cagcgaagct tcccggctgg
     3901 ctgtgcatag tgcaatggag tctcttagct atcacccgaa cgccaacgcc cgtgcgctgg
     3961 cgcagcagac cactgaaacg gtcggtctga tcgttggtga tgtttccgat ccgtttttcg
     4021 gtgcaatggt gaaagcggtc gaacaggtgg cttatcacac cggtaatttc ttattgattg
     4081 gcaacggtta ccacaacgaa caaaaagagc gtcaggctat tgagcaactg atccgccatc
     4141 gctgtgctgc gttggtcgtc catgccaaaa tgatcccgga tgccgattta gcctcattaa
     4201 tgaaacaaat gcccggtatg gtgctgatca accgtatcct gcctggcttt gaaaaccgtt
     4261 gtattgctct tgacgatcgt tacggtgcct ggctggcaac gcgtcattta attcagcaag
     4321 gtcatacccg cattggttat ctgtgctcta accactctat ttctgacgcc gaagatcgtc
     4381 tgcaagggta ttacgatgcc cttgctgaaa gtggtattgc ggccaatgac cggctggtga
     4441 catttggcga accagacgaa agcggcggcg aacaggcaat gaccgagctt ttgggacgag
     4501 gaagaaattt cactgcggta gcctgttata acgattcaat ggcggcgggt gcgatgggcg
     4561 ttctcaatga taatggtatt gatgtaccgg gtgagatttc gttaattggc tttgatgatg
     4621 tgctggtgtc acgctatgtg cgtccgcgcc tgaccaccgt gcgttaccca atcgtgacga
     4681 tggcgaccca ggctgccgaa ctggctttgg cgctggcgga taatcgccct ctcccggaaa
     4741 tcactaatgt ctttagtccg acgctggtac gtcgtcattc agtgtcaact ccgtcgctgg
     4801 aggcaagtca tcatgcaacc agcgactaac cgcagttaaa gcaattccag cgccagtaat
     4861 tcttcgatgg tctggcgacg gcgaatcaac cgcgcctgac cattatcaaa cagaacttct
     4921 ggtaacagcg aacggctatt gtagttggat gacattgatg cgccatatgc ccctgtatca
     4981 tgcagtacca gataatcacc tgccttcact tccggcaagg cgcgggtttc aacatttccc
     5041 ccttcctgct gggtaaagac atcgcccgat tcacataacg gtccggcgac gacggtttcc
     5101 accgttggcg cgtgttccag agaacgacca tcagctgcca gggcactgat atggtggtaa
     5161 ctaccgtaca ttgccgggcg catcagatcg ttgaacccgg catcaaccag cacaaagtgg
     5221 cggctcccca tttgtttgac gctccgcacc tgagtaatta atacgccaga ctgcgctacc
     5281 aggaagcgac ccggttcaat ttccagtttc acagggtggc ccaaatggcg ggcgatttgc
     5341 tcacgcgcgg cattccacag accataataa tgttcggtat caaccgcctc ttcaccctgt
     5401 tgataaggaa cagaaagccc accgcccgca gaaatagcct gtaaatcctg accgaattcg
     5461 atgacctgac gcaccatagc accacacacc tgttccagat gggcataatc aacgccagaa
     5521 ccaatgtgca tgtgaatgcc gaccagctgc agatgatgac gttgtatcac gtccagtgcg
     5581 gcgggcagat cggtgtacca gataccgtgc ttgctgtttt cgccaccggt attggttttt
     5641 tggctatgtc cgtgaccaaa ccccggatta acgcgcagcc atacccgatg ccctggcgaa
     5701 accgggccca gttggtcgag catatcaaca gaacccgcat tcaccggaat ttgcaattca
     5761 ctgacgcgtt caagcgtcgc ctgatcgata acatctgccg taaaaacaat atcatcgggg
     5821 tgcgtttgcg gattgtaacc cgccgccaac gcacgctcta tttcgcctaa cgagacggaa
     5881 tccactttca cgccctgctc acgcattaag cgcaaaatat gaatattgga acaggctttc
     5941 tgtgcaaagc gcaccacatc aaactgtttc agcgctgcaa tctgccgacg aataatttgc
     6001 gcatcgtaga cccacaccgg gcagccaaat tcagcgggca aacgcagcag attttcggcg
     6061 gtgagatcgg tatcggtgct gaacagtgaa tgtggcataa caaactccag ataagtgctt
     6121 ttttatgatt acgccacatc ataaaaagaa taaaaaatat cgatttatgt cgagtctatg
     6181 caaaaatgat atggattacc ggattgcgag agagcgctaa tggccgccgt taacttacgt
     6241 catattgaaa tttttcatgc ggtaatgacc gccggaagcc tgactgaggc ggcacacctg
     6301 ctacacacct cacagccaac cgtcagccgc gaacttgcgc gctttgagaa ggtgatcggg
     6361 ctgaaattgt ttgagcgcgt acgtgggcga ttacatccta ccgtgcaagg actgcgtctg
     6421 tttgaagaag tgcaacgatc ctggtacgga ctggatcgaa ttgtcagcgc tgcagaaagt
     6481 ctgcgcgagt ttcgccaggg agaactgtct attgcctgcc tgccggtctt ttcgcaatct
     6541 tttttaccgc agctcctgca accctttctg gcacgttatc ccgatgtcag cttaaatatc
     6601 gtgccccagg aatcaccgct acttgaagag tggctctcgg cccagcgtca tgatttagga
     6661 ctcactgaga cactccatac gcctgcggga acagaacgta ccgaattact ctctttagat
     6721 gaagtgtgtg tgttacctcc gggtcatccg ctggcggtaa aaaaggtatt aacgccggat
     6781 gattttcagg gtgagaacta catcagcctt tcccgtactg acagctatcg ccagttgctg
     6841 gatcagctat ttactgaaca tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc
     6901 gcgtcaatct gcgcaatggt acgggcgggg gtaggtgttt cggtggttaa cccgctcacc
     6961 gcactggatt atgcggcaag cggtttagtg gtgcggcggt tcagtattgc ggttccgttc
     7021 accgtcagcc tgatccgccc cctgcaccgc ccgtcatcag cgctggtgca ggcgtttagt
     7081 gagcatttac aagcggggtt accgaaactg gtcacttctc ttgacgctat tttgtcgtca
     7141 gctacgacag cataaaagcg acagcatcct cggcatggat cgccgcagta tcaaacacag
     7201 gcaaaacact gcgctcttct ggcaccagta aaccaatttc tgtgcagcca aaaatgacgc
     7261 cctgtgcgcc ctgttctgca aggcgagcaa tcacttgcgc ataataagcg cgtgacgctt
     7321 cggtaaattg ccccagacac agttcttcaa aaataatctg attaattttc gcccgttcat
     7381 ccgcttcagg aataagacaa ttgatggaaa attgttccgt cagccgcccg cgataaaaat
     7441 cctgttccat ggtgtaacgc gtacccagca gcgccacacg agtcattccg gccccggtaa
     7501 ttgcacgtcc ggtggcatcc gcaatgtgta agaaaggcag agtgcaacgt gactcaatgg
     7561 catccgccac tttatgcatc gtattggtac ataacacaat accttctgcg cccgcccgct
     7621 gtaagccaag cgccgcctca gccagaatgt ccccggtttt atcccattcc ccgcgacgct
     7681 ggcactcttc tatttcatga aaatcgacgc tatgtagcag cacttgcgca gagtgaagcc
     7741 caccaagccg ctgtttaatg ccttcattta tcaaacgata gtaaggaatg gtggattccc
     7801 agctcattcc tcccagcaaa ccaattgttt tcattcctct ctcctgatgt gtgttaccgc
     7861 aacaaggctg aataaggaac gagacaaacg cctcaacggc caagtgccca atctctatta
     7921 acgaaaaaag ggccggatgt acagcacatc cggcccgtga aatcagatgc cgatatttct
     7981 caacttctcg cctgccatca gtttgcgttc gatatgttcc agcgtgacat ttttggtttc
     8041 cggaatgagc cagaaagtga tgcccacaaa cgcaatgttc agcgcagtgt agagccagaa
     8101 cgtaccggca gcgccaatgc tgtcgagcag cgtcaggaag gtcgcgccga taatcatatt
     8161 cgacacccag ttcgtggtgg tcgaacaggt aataccgaaa tcgcggcatt tcagcggctg
     8221 aatttcagag cacaggatcc acaccactgg cgcggcgctc atcgcataac cggcaataca
     8281 catcatcgtc atgccaacag agagccagga caagccactg gaagccgtac cgttatcaaa
     8341 ctgcatcagg caatagccca gcaccagagt gcctaacgcc atcacgctga aaccaatttt
     8401 cagagccggt ttacgccctg ctttatctac cgtaaacacc gcaataaagg tggcgaacat
     8461 aaaggtcagc cctacgacca gagtcgtaat catctgttgt tctgtggtcg taaagcccgc
     8521 cattttgaag atacgcggcg cgtagtacat gatgatgttc ataccggtaa actgctgcat
     8581 cgcctgcaac aacataccga gaaacacagc acgacggacg ttacggttga tcttaaacag
     8641 tgcccaaccg ccctgtttta acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc
     8701 tttttccgac gtatcgcgca gcatacgcaa tacttcttcc gcctcaatat gacgcccctt
     8761 ttccgccaac cagcgcgggc tatttggcag gaagaccacc agaataatca gcagaactgc
     8821 tggtaaagca agaaccccca acattgcgcg ccagttaccg ctataactga acgctgtatc
     8881 ggataaaaac gccagcacga tgccgagtgt gaccatcaac tggtacatac tgatcatctt
     8941 accgcgaacg ttttcacttg ccatttcaga aagatacaga ggagcggtgt aagacgcgat
     9001 cccgacagca atgcccagca ccacacgagc ggcgattaac atctctacac tggtcgcaaa
     9061 agcggaccct atagaaccga gtacaaacag gatggccccc gccatcaggc tgtatttacg
     9121 ccccaggcgg aacgacagcc aaccattaaa cagcgcacca attgctgcgc cgagcatcat
     9181 gctactaacc acccattcct gcaaatgact ggtcagcaca aagtgatcgg taatgaacgg
     9241 caacgctccg gcgattacgc cgatatcaag accaaataac aatcctgcga ccgcagcagc
     9301 taccgaaaca aacatattca tacgccgcgt atcccgcaaa gaacgtggcg ttaaagcaga
     9361 ttccgtattg atagtaacca ttttttcctg ccagcagaga gtaagacata gtgaaaaaat
     9421 acgtgaacaa ctcacgcagg tgtcaggtcg gaaacagcat aaatatggat taaattgctg
     9481 cgacatgtcg ttatgtgatg gatattccaa ttttcaaatt aagttgaatt attgagatta
     9541 ttattaacca cctaatttta cagcagataa aattcataaa gttcattaat tgataattaa
     9601 tatggattat ttcataacca tgatatggat tatgatgatc tacaggtata aaaaaccctg
     9661 ccatgcggca gggtcataaa agtaagaaga atgaattaac gcgccagcca accgccatcc
     9721 acggcaatgg tataaccatt cacataatct gaagcgctgg aggcaaggaa cactatcggc
     9781 cccatcaggt cactcggcag tccccaacga ccagctggaa tgcggtcgag aatttccgcg
     9841 ctacgttgtt catctgcccg tagttgttga gtattgttgg tcgccatgta acccggagct
     9901 atcgcattaa cattaatgtt gtgtttagcc cattcgttcg ccatcaatcg cgtcacaccc
     9961 atcacgccgc tttttgatgc ggtataagaa ggcacacgga tcccgccctg gaaggagagc
    10021 attgacgcga tattgataat cttgccgcca ttgccttgcg cgataaagtg tttcgccgct
    10081 gcctgagaca tgaagaatac gctcttgata ttcaggttca tgacatcgtc ccagtctttt
    10141 tcgctgaact cgagagcgtc ttcgcggcga atcaatccgg cgttattcac caggatatca
    10201 atatgaccaa attcagctac cgcgcgatcc agcagtccag gaatgccatc aatctttcgc
    10261 agatcggcgg tcaggcttaa aaaacgacgc cccagcgccg tgacctgctc gatggtttca
    10321 gtcggttcaa cgatgttaat gccaacaatg tcacagcccg cttgcgccag ccccaacgcc
    10381 atcccctggc ccagcccagt atcacaacca gtgacgaccg caactttacc ttcgagagaa
    10441 aatgcactta aaatcataac aataccttat tcgtttatgc ccacaactag cgcaaatctt
    10501 taacggccac atggtccata tcatcaaaga cctggttttc accaaccatg ccccagataa
    10561 aggtataagc tttggttccg acgccggaat ggatcgacca gctcggggag atcaccgcct
    10621 gctcgttatg catcacaatg tgacgcgttt cttgcggttg ccccatcatg tggaaaacgc
    10681 aggcgtcatc atccatattg aaatagaaat aaacttccat ccggcgctcg tgggtgtgac
    10741 acggcatggt gttccacaag ttacctggag ccagctccgt cagccccata ctcaattggc
    10801 aggtttccag cacatccggg acaaaatatt tgttaatagt gcgacggtta ctggtgaggt
    10861 tatcgcctaa cgtgactgga gatacttcgt ccggggtgac ttttttggtg ggatacgtcg
    10921 tatgcgcggg tgcgcaattg taataaaact ttgccggagt ggcggtatca atactggcaa
    10981 agacaacttc ttttgcacct ttaccaacat acagggcatc gcggtgaccg atttcatagc
    11041 attggccatc gacagtaatc gtaccggcac cgccaatatt gataacacct aactcgcgac
    11101 gttcaaggaa atagcgtacg ccgagttgtt taccaacttc cccgccaacg gaaaccgttt
    11161 tagttatcgg cataatgccg ccaacaataa ttcggtcaat gtggctgtaa accatggtgt
    11221 actcatcggc gacaaatacc ttttcaacca aaaattcatt gtgcagccct tgggtatcca
    11281 gcgtttttgc gtgcgcactg tggatgctct gtcttacgtc cacatcaacc tccgataatc
    11341 agtgaagcgt gaaaatagaa aacgatagtg aaacactgtt tcgttttctc tataaaggga
    11401 tgatatgccc atgggctagg gttttcaatt aaaaataaaa caatgtttta tttttaagtt
    11461 aaggattaaa aaaagtgcag tgtcgatcac gaatatgtca tgagaatgga aaaatgaggg
    11521 agcccagaaa atgtatgtcg acttgccagc catcattatt caacagatta atgacggcag
    11581 atatttatcg gtaaggagga aatgcagagc tactcgtcac gttcaatggt caatgccacg
    11641 ccctggcccc cgccgataca aagcgttgcc agccctttgc gggcattacg tttcaccatt
    11701 tcatgaacca gagaaaccag gattcggcaa ccggaagcgc ctatcgggtg accgagtgcg
    11761 atcgcgccac cattgacatt gacccgacgc tcatcccact caagcatctt gccaaccgaa
    11821 agcgcctgtg cagcaaacgc ttcattagcc tcgataagat cgacatcagc caactgccag
    11881 cctacacgct ccaggcaacg gcgggtcgca tacaccggcg caattcccat caatgccgga
    11941 tctacaccaa cgctggcaaa tgcgcggatg cgggccagca cgggtaaatt caacgctcgt
    12001 gctttggctt cgctcatcat cattaccgca gctgcgccat cgtttatgga tgatgcattc
    12061 cccgcggtca cagaaccgag actatcaaat gaaggattta aacgggctaa accttctgca
    12121 ctggcgtcag tgcatggctg ttcatcggta tcaacaacca acgtctgacc gttactttgg
    12181 atcattaccg ggacgatctc atctttaaat cgtccggcgt caatcgccgc tcgcgctttt
    12241 tgttgcgagc taagtgcgta agcatcctgc aactgacggc tgatgccata ttcgcgagcc
    12301 agattttcgg cggtgacacc aatatgataa tcattgaagg catcccacaa cccatcatgc
    12361 accagactgt caaccaactg gctattgcca agctgtgcac cggtgcggct atcagtcaga
    12421 acatgtggtg cgcggctcat gttttcctgg ccaccggcga tgacaatatc agcctcgcca
    12481 cactgtatcg cctgagtagc cagatgcagt gctttaagcc cagaaccgca aacgtcatta
    12541 atagtgattg cagaaacgct attaggaaga ccacctttaa tagccgattg ccttgccgga
    12601 ttctgccctg cccctgcagt caacacctga ccaagaatta cttcatccac cgcatatgca
    12661 ggaacgccgg tacgttctat taacgctttc acgaccaaac taccaagttc cacggcggaa
    12721 tgacccgcta acgcaccacg aaagcagccg ataggtgtcc gtaacgcccc gacaatcaca
    12781 acgtctttca tcacaacctc gcaccaatta acagtgcaat agtaaatgat tgttaattac
    12841 agttatctta attgtttaaa aaaagtgatt tttatcacaa aggaaatatg cctgagcagc
    12901 agtcagagac ataactggca cgtaaggttt gcaaccacta acccaccaat agaggggtag
    12961 atggggcgtt aatctcccat acttaacctg gtttatggta aattgccctc cattttgttt
    13021 aatttgtaga tgatacgttc agataatgtc taatatttgg tctaaagaag aaactctgtg
    13081 gagtttcgcg ctctacggca cagccgttgg tgcaggcacg ctcttccttc ctattcagtt
    13141 aggttcggca ggggctgtgg tcctgtttat tactgctctg gtcgcctggc ctttaacata
    13201 ttggccacat aaagctttat gccagttcat cctctcatcg aaaacatcag caggtgaagg
    13261 gataacgggc gcggtaacac actactatgg caagaagatt ggtaatctga ttaccacgct
    13321 gtacttcatc gccttttttg tcgtcgtgtt gatatatgca gtggcaatta ccaactcact
    13381 tacagaacag ctggcaaagc atatggttat tgatcttcgc atccgtatgt tggtgagtct
    13441 gggtgttgta ttaatcctga atctcatttt tctgatggga cgccatgcaa ccattcgggt
    13501 aatgggtttt ttggtattcc cattgattgc ctatttctta tttctttcca tttaccttgt
    13561 cggtagttgg caacctgatc tattaacgac ccaggtagag ttcaatcaga atccccttca
    13621 ccagatatgg atatcgattc ccgtgatggt tttcgccttt agccatacgc ccattatttc
    13681 tacgtttgcc atagacagac gtgaaaaata tggcgaacac gctatggata aatgcaaaaa
    13741 aattatgaaa gtcgcttatc tcatcatctg cataagtgta ctgttctttg tctttagctg
    13801 cctgctttct attccacctt cgtatattga agcggctaaa gaagaagggg tcaccatttt
    13861 atcggcgctt tctatgctgc cgaacgcccc agcatggttg tcaatttccg ggattattgt
    13921 cgcagtagtt gcgatgtcga aatcattcct gggtacgtac tttggcgtta ttgaaggtgc
    13981 cacggaggtc gtcaaaacaa cactacagca ggttggtgta aagaaaagtc gtgcatttaa
    14041 ccgcgcacta tcaattatgt tggtatcgct gattaccttc attgtttgtt gcattaaccc
    14101 gaacgcgatt tcgatgattt atgcgatcag cggcccgctc attgccatga tacttttcat
    14161 catgcctacg ttgtcaacgt atctgatccc ggcgcttaaa ccctggcgct ccatcggaaa
    14221 tctgattacg ctgatcgtgg gtatcctgtg cgtatcggta atgttcttta gct
//
LOCUS       DAJTNA010000097        14083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTNA010000097 DAJTNA010000000
VERSION     DAJTNA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14083
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.098"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(177..389)
                     /gene="rpmE"
                     /locus_tag="OO161_002491"
     CDS             complement(177..389)
                     /gene="rpmE"
                     /locus_tag="OO161_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5463529.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            592..2790
                     /gene="priA"
                     /locus_tag="OO161_002492"
     CDS             592..2790
                     /gene="priA"
                     /locus_tag="OO161_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5463530.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSLKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2946..3971
                     /gene="cytR"
                     /locus_tag="OO161_002493"
     CDS             2946..3971
                     /gene="cytR"
                     /locus_tag="OO161_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5463531.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4063..5022
                     /gene="ftsN"
                     /locus_tag="OO161_002494"
     CDS             4063..5022
                     /gene="ftsN"
                     /locus_tag="OO161_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5463532.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAL
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5115..5645
                     /gene="hslV"
                     /locus_tag="OO161_002495"
     CDS             5115..5645
                     /gene="hslV"
                     /locus_tag="OO161_002495"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5463533.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5655..6986
                     /gene="hslU"
                     /locus_tag="OO161_002496"
     CDS             5655..6986
                     /gene="hslU"
                     /locus_tag="OO161_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5463534.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7053..7979
                     /gene="menA"
                     /locus_tag="OO161_002497"
     CDS             7053..7979
                     /gene="menA"
                     /locus_tag="OO161_002497"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5463535.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8072..8557
                     /gene="rraA"
                     /locus_tag="OO161_002498"
     CDS             8072..8557
                     /gene="rraA"
                     /locus_tag="OO161_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5463536.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8642..8887)
                     /gene="zapB"
                     /locus_tag="OO161_002499"
     CDS             complement(8642..8887)
                     /gene="zapB"
                     /locus_tag="OO161_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5463537.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9313..10158
                     /gene="glpF"
                     /locus_tag="OO161_002500"
     CDS             9313..10158
                     /gene="glpF"
                     /locus_tag="OO161_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR5463538.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10181..11689
                     /gene="glpK"
                     /locus_tag="OO161_002501"
     CDS             10181..11689
                     /gene="glpK"
                     /locus_tag="OO161_002501"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5463539.1"
                     /translation="MAEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11824..12834
                     /gene="glpX"
                     /locus_tag="OO161_002502"
     CDS             11824..12834
                     /gene="glpX"
                     /locus_tag="OO161_002502"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5463540.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGKQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12931..13677
                     /gene="fpr"
                     /locus_tag="OO161_002503"
     CDS             12931..13677
                     /gene="fpr"
                     /locus_tag="OO161_002503"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5463541.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     HDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13682..>14083)
                     /gene="uspD"
                     /locus_tag="OO161_002504"
     CDS             complement(13682..>14083)
                     /gene="uspD"
                     /locus_tag="OO161_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5463542.1"
                     /translation="PSGNEEDALLVNKALELARHNDAHLTLIHIDDGLSELYPGIYFP
                     ATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKEQCDLLVCGH
                     HHSFINRLMPAYRGMINKMSADLLIVPFIDK"
BASE COUNT         3383 a         3620 c         3812 g         3268 t
ORIGIN      
        1 gccggttttc cgctcatcgt aaaatccttt tgaaataaat aagaaccaga agcctagcgc
       61 cagtcaacaa caggaaatga taatgagtta acaccccgga atttttcaga atttataaaa
      121 ggcaaaattc aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat
      181 ttgctgcccg ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca
      241 tcacgctgtt tgccagtaaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca
      301 tgaccaacag tggagcggat tttcattacg ttaccgcaag agcagctagc agtaatttct
      361 tcgtatttcg ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc
      421 ttccagccct aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt
      481 cgtacgaagg cgcgaaatca tacagaaatt aaccagcgta tgcaaactga tccgcactct
      541 tctacggcaa tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt
      601 gcccacgttg ccttgcccgt tccgcttcct cgtacctttg actatctgct gccagaaggc
      661 atgacggtta aagccgggtg tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc
      721 ggggttgtgg tgtcagttag cgatgtcagt gagctgccgc ttaacgagct gaaagcggta
      781 gtggaagtgc tggacgttga gccggtattt actcactccg tctggcggtt actgctctgg
      841 gcggcagatt actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta
      901 ctgcgccagg ggcggcctgc ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa
      961 ggccaggcgg tggatctgaa cagcctgaaa cgctccccca agcaacaaca ggcgctggcg
     1021 gcgttacggc aaggcaaaat ctggcgcgac caggtcgcca cgctcgaatt taatgatgcc
     1081 gcgttgcagg cgctacgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt
     1141 agcgactggc gaacgaacta tgccgtttct ggtgagcggt tgcgattgaa taccgaacag
     1201 gccaccgccg ttggcgcaat tcatagcgcg gcagatactt tttctgcctg gctgctggcg
     1261 ggcgttaccg gttccggtaa aacggaggtt tatctcagtg tactggaaaa cgtgctcgct
     1321 cagggcaaac aggcgctggt gatggtgccg gaaatcggcc tgacaccgca aactatcgcc
     1381 cgttttcgtg aacgttttaa tgcccccgtg gaagttctgc attccggcct gaacgacagc
     1441 gagcggcttt cggcgtggtt gaaagcgaaa aatggcgagg cggcgattgt gatcggcacc
     1501 cgctccgcgc tgtttacgcc gtttaaaaat ctcggcgtga ttgtcattga tgaagagcac
     1561 gacagctcct acaagcagca ggaaggctgg cgctatcatg cccgcgacct ggcggtgtat
     1621 cgtgcgcaca gcgagcaaat cccgattatt cttggctccg caacgcccgc gctggaaacg
     1681 ttatgcaacg tccagcagaa aaaataccgc ctgctgcgcc tgacccgtcg ggcagggaat
     1741 gcgcgtccgg caattcaaca tgtgctggat ttaaaaggtc agaaggtgca agcaggtctg
     1801 gctccggcgt taatcacccg tatgcgccag catttacagg ccaacaatca ggttatcctc
     1861 tttcttaatc gtcggggttt tgcgcctgcg ctgctgtgcc acgactgcgg ctggatagcc
     1921 gaatgcccac gttgcgatca ctactacacg ctgcatcagg cgcaacagca tttgcgctgt
     1981 caccactgtg acagtcagcg tccggtgccg cgccagtgcc cttcctgcgg ttccacgcac
     2041 ctggtccccg tggggctggg caccgaacag cttgaacaga cgcttgcgcc gatgttcccc
     2101 ggcgtgccca tttcacgtat cgaccgcgat accaccagcc tcaaaggggc gctggaacag
     2161 caactggcgg aagtgcatcg cggcggcgcg aggattttga tcggcacaca aatgctggcg
     2221 aaaggtcacc actttccgga tgtgacgctg gtggcgttac tggatgtgga cggcgcgctg
     2281 ttttctgccg attttcgctc ggcagaacgt ttcgcccagc tttacaccca ggtcgccggt
     2341 cgtgccgggc gtgcgggtaa acagggcgaa gtggtgctgc aaacgcacca tccggaacat
     2401 cctctgttgc aaacgttgct ctataaaggc tacgacgcct ttgccgaaca ggcgctggct
     2461 gagcgacgca tgatgcagct gccgccgtgg accagccatg tgattgtgcg tgcggaagat
     2521 cataacaatc agcacgcgcc gctgttcctg caacaactgc gtaatctgat cctttccagc
     2581 ccgctggcag acgacaaact gtggattctc ggcccggttc cggctctggc acctaagcgt
     2641 ggtggccgct ggcgctggca gatcttgttg cagcaccctt cccgcgtgcg cttgcaacac
     2701 atcattagtg gtacgctggc gcttattaat acaataccgg attcccgcaa ggtgaaatgg
     2761 gtgttggatg tcgatcccat cgaaggctaa tgcgcaaacg ttgcgaggcg gatcgaaaaa
     2821 ttcaatattc atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta
     2881 aaaatgttgc cgatgtgaag taaacatgga tgtagtacgc ctgacgtacc aggcgaggag
     2941 tgagtgtgaa agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg
     3001 caaaagtctc tacagcgact gtctcccgag cattaatgaa tcccgataaa gtctcccagg
     3061 ccacccgtaa tcgggttgaa aaagcggccc gggaagtggg ttatttaccg cagcctatgg
     3121 ggcgcaacgt caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg
     3181 atcccttctt tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc
     3241 tggtgctgat tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga
     3301 tcatcaccaa gcaaattgat ggcatgttgc tgctgggttc aaggctgccg tttgatgcca
     3361 gcattgagga acagcgtaat ctgccgccga tggtgatggc gaacgaattt gcaccggaac
     3421 tggagctgcc taccgttcat atcgacaatc tgaccgccgc atttgatgca gtaaattatt
     3481 tatatgagca agggcataaa cggattggct gtatagccgg tcccgaagag atgccactgt
     3541 gtcactaccg cctgcaaggc tatgttcagg cgctgcgtcg ctgcggaatt atggttgatc
     3601 cacaatacat cgcccgtggc gacttcacct tcgaagccgg aagcaaagca atgcaacagc
     3661 tgcttgatct tccacaaccg cctactgcag tcttctgcca tagcgatgtg atggcgctcg
     3721 gcgcactttc tcaggcaaaa cgccaggggc tgaaagtccc ggaagacctt tccataatcg
     3781 gttttgataa catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc
     3841 cgcgttacga aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcaac
     3901 acgttggcag tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg
     3961 cgttacctta aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt
     4021 aacatggcgg gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat
     4081 gtacgccgca gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa
     4141 cgaaatctgc ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc
     4201 tttatcggtg gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa
     4261 agccagaaag tgaccggaaa cggactacca ccaaaaccag aagaacgctg gcgctacatt
     4321 aaagagctgg aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgccggtggt
     4381 gaagtgaaaa cgccggagca actgacacca gaacaacgtc agcttcttga acaaatgcag
     4441 gctgatatgc gccagcagcc aacgcagctg gttgaggttc cgtggaatga acagacgccg
     4501 gaacagcgtc agcaaacgct acaacgccag cgtcaggcgc tgcaattagc ggagcagcaa
     4561 cgcctggctc agcagtcgcg tacgactgaa caaagctggc agcagcagac gcgtacgtcg
     4621 caagccgcgc cggtacaggc gcaaccgcgc cagtctaaac cagcttctac tcagcagccg
     4681 taccaggatc tgctgcaaac ccctgcgcac acgaccgcgc agtcgaagcc gcagcaagct
     4741 gcgccggttg ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc
     4801 cgctggatgg tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct
     4861 cagctggcgt tcgaaggatt tgactcgaaa atcaccacca acaatggctg gaatcgtgtg
     4921 gtcattggcc cggtgaaagg caaagagaac gcagacagca ccctcaatcg gttgaagatg
     4981 gcgggtcata caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc
     5041 catctataat tgcattatgc cccgtacttt tgtacggggt ttgtactctg tattcgtaac
     5101 caaggggtca gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg
     5161 ctggtgatgg tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc
     5221 gccgtctgta caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta
     5281 cgctgttcga actgtttgaa cgtaaactgg aaatgcatca gggccatctg gttaaagccg
     5341 ccgttgagct ggcaaaagac tggcgtaccg atcgcatgct gcgcaaactt gaagcactgc
     5401 tggcagtcgc ggatgaaact gcatcgctta tcatcaccgg taacggtgac gtggtgcagc
     5461 cagaaaacga tcttattgct atcggctccg gcggccctta cgcccaggct gcggcgcgcg
     5521 cgctgttaga aaacactgaa cttagcgccc gtgaaattgc tgaaaaggcg ttggatattg
     5581 caggcgacat ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag
     5641 cgtaaggatc tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc
     5701 acatcatcgg ccaggacaac gccaagcgtt ctgtggcgat tgctctgcgt aaccgctggc
     5761 gtcgcatgca gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga
     5821 tcggcccgac cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg
     5881 cgccgttcat caaagttgaa gcgaccaaat tcaccgaagt gggctatgtc ggtaaggaag
     5941 tggattctat tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta
     6001 tcgagaaaaa ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtgctga
     6061 tcccacctgc taaaaacaac tggggacaga ccgaacagca gcaggaaccg tccgctgctc
     6121 gtcaggcatt ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg
     6181 atcttgccgc agcaccgatg ggcgttgaaa ttatggctcc tccaggcatg gaagagatga
     6241 ccagccagct gcagtccatg ttccagaacc tgggcggcca gaagcaaaaa gcgcgtaagc
     6301 tgaaaatcaa agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc
     6361 cggaagagct gaagcaagac gctatcgacg ctgttgagca gcacgggatc gtgtttatcg
     6421 acgaaatcga caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag
     6481 gcgttcagcg tgacctgctg ccgctggtag aaggttgcac cgtttccacc aaacacggga
     6541 tggtcaaaac tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt
     6601 ctgacctaat cccggaactg caaggtcgtc tgccaatccg cgttgaactg caggcactga
     6661 ccaccagcga cttcgagcgt attctgaccg agccgaatgc ctctatcacc gtgcagtaca
     6721 aagcactgat ggcgactgaa ggcgtaaata tcgagtttac cgactccggt attaaacgca
     6781 tcgcggaagc ggcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac
     6841 acactgttct ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc
     6901 aaaatatcac tattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg
     6961 aagatctgag ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg
     7021 gctgaaaggc cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc
     7081 aggcgtggct ggaaagttta cgacctaaaa ccctccccct cgcctttgct gcaattatcg
     7141 tcgggacggc gctggcatgg tggcaaggtc attttgatcc gctggtcgcc ctgctggcac
     7201 tgattaccgc cgggctatta cagatccttt ctaacctcgc caatgattac ggcgatgcgg
     7261 taaaaggcag cgataaacct gaccgcattg ggccgctacg cggcatgcaa aaaggggtca
     7321 ttacccagca agagatgaaa cgggcgctca ttattaccgt cgtgctcatc tgtctctccg
     7381 ggctggcact ggttgcagtg gcgtgccata cgctggctga ttttgtcggt ttcctgattc
     7441 ttggcgggtt gtcgatcatt gccgctatca cctacaccgt gggcaatcgt ccttatggtt
     7501 atatcggtct gggggatatt tccgtactgg ttttctttgg ctggttaagc gtcatgggga
     7561 gctggtattt acaggctcat acattgattc cggcactgat ccttccggcg accgcatgcg
     7621 gcctgctggc aacggcagta ctgaacatta ataacctgcg tgatatcaat agcgaccgcg
     7681 aaaacggcaa aaacacgctg gtggtgcgct taggtgaagt gaacgcgcgt cgttatcatg
     7741 cctgcctgct gatgggctct ctggtatgtc tggcgctgtt taatctcttt tcgctgcata
     7801 gcctgtgggg ctggctgttc ctgctggcgg caccgttgct ggtgaagcaa gcccgttatg
     7861 tgatgcggga aatggacccg gtggcgatgc gaccgatgct ggaacgcact gtcaaggggg
     7921 cgttactgac taacctgctg tttattttag ggatattcct aagccagtgg gcagcataac
     7981 tgacaaatat caattaacaa ttgatgattt tgccaacagc ccacatagcg cgatatactg
     8041 aaaattctcg cagcaactga atgttaagcc tatgaaatac gatacttccg agctttgtga
     8101 catctatcaa gaagatgtta acgtcgtgga accgctgttc tccaactttg gcggacgggc
     8161 gtcgtttggc ggacaaataa tcacggtaaa atgtttcgag gacaacgggt tgctgtacga
     8221 tctgctcgaa cagaatggcc gtggtcgtgt tcttgtcgtc gatggcggtg gttctgttcg
     8281 tcgcgcactg gtcgatgctg aactggcgcg tctggcagta caaaatgaat gggaaggtct
     8341 ggttatttac ggcgcggtgc gtcaggtaga tgacctggaa gagttggata tcggcatcca
     8401 ggcgatggcg gcaattccgg ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg
     8461 cgtcaatttt ggcggtgtaa ccttcttctc cggcgaccat ctttatgccg acaataccgg
     8521 gattattctt tcagaagatc cgctggatat tgaatgataa gaaaggcacc gcaaggtgcc
     8581 ttttttctgc gttacctgtt ggcctacaca gtaaagaaat tacgcgaaag atgaagcgta
     8641 atcagacctc ttccatgcga cccagcaggg cctgcagacg ttcctgccag ccgttctgct
     8701 gttctttcag atggttgttc tcacgctcca gctcttcgcg ctgatgctgg gcattttgaa
     8761 cttcctgcga cagtgagttg tttttttctt tcagctcttc gatttccatc tgcaacagag
     8821 tgatggtatc aatcgcctgc tgtacttttg cttccagttt ctcaaacact tctaatgaca
     8881 ttgtcatacc tctcctgaat tgcaaggcgt tgatggataa aaaatcctcg tcccgattac
     8941 cggtgacgcc ttaataaata cgagcgcact ttagttagct ccgattgtat gaagccgcgc
     9001 catcgctgtc cagcggcacg ccttgcagat tacggtttgc cacacttttc atccttctcc
     9061 tagtgacata atccacatca atcgaaaatg ttaataaatt tgttgcgcga atgatctaac
     9121 aaacatgcat catgtacaat cagatggaat aaatggcgcg ataacgctca ttttatgacg
     9181 aagcacacac attttaagtt cgatatttct cgtttttgct cgttaacgat aagtttacag
     9241 catgcctaca agcatcgtgg aggtccgtga ctttcacgca tacaacaaac attaactctt
     9301 caggatccga ttatgagtca aacatcaacc ttgaaaggcc agtgcattgc tgaattcctc
     9361 ggtaccgggt tgttgatttt tttcggtgtg ggttgcgttg cagcactaaa agtcgctggt
     9421 gcgtcttttg gtcagtggga aatcagtgtc atttggggac tgggggtggc aatggctatc
     9481 tacctgaccg caggggtttc cggcgcgcat cttaatcccg ctgttaccat tgcattgtgg
     9541 ctgtttgcct gtttcgacaa gcgcaaagtt attcctttta tcgtttcaca agttgccggc
     9601 gctttctgtg ctgcggcttt agtttacggg ctttactaca atttattttt cgacttcgag
     9661 cagactcatc acattgttcg cggcagcgtt gaaagtgttg atctggcagg cactttctct
     9721 acttacccta atcctcatat caattttgtg caggctttcg cagttgagat ggtgattacc
     9781 gctattctga tggggctgat cctggcgtta acggacgatg gcaacggtgt accacgcggc
     9841 cctttggctc ccttgctgat tggtctactg attgcggtca ttggcgcatc tatgggccca
     9901 ttgacgggtt ttgccatgaa cccagcgcgt gacttcggtc cgaaagtctt tgcctggctg
     9961 gcgggctggg gcaatgtcgc ctttaccggc ggcagagaca ttccttactt cctggtgccg
    10021 ctttttggcc ctatcgttgg cgcgattgta ggtgcatttg cctaccgcaa actgattggt
    10081 cgccatttgc cttgcgatat ctgtgttgtg gaagaaaagg aaaccacaac tccttcagaa
    10141 caaaaagctt cgctgtaata tgactacggg acaattaaac atggctgaaa aaaaatatat
    10201 cgttgcgctc gaccagggca ccaccagctc ccgcgcggtc gtaatggatc acgatgccaa
    10261 tatcattagc gtgtcgcagc gcgaatttga gcaaatctac ccaaaaccag gttgggtaga
    10321 acacgaccca atggaaatct gggccaccca aagctccacg ctggtagaag tgctggcgaa
    10381 agccgatatc agttccgatc aaattgcagc tatcggtatt acgaaccagc gtgaaaccac
    10441 tattgtctgg gaaaaagaaa ccggcaagcc tatctataac gccattgtct ggcagtgccg
    10501 tcgtaccgca gaaatctgcg agcatttaaa acgtgacggt ttagaagatt atatccgcag
    10561 caataccggt ctggtgattg acccgtactt ttctggcacc aaagtgaagt ggatcctcga
    10621 ccatgtggaa ggctctcgcg agcgtgcacg tcgtggtgaa ttgctgtttg gtacggttga
    10681 tacgtggctt atctggaaaa tgactcaggg ccgtgtccat gtgaccgatt acaccaacgc
    10741 ctctcgtacc atgttgttca acatccatac cctggactgg gacgacaaaa tgctggaagt
    10801 gctggatatt ccgcgcgaga tgctgccaga agtgcgtcgt tcttccgaag tatacggtca
    10861 gactaacatt ggcggcaaag gcggcacgcg tattccaatc tccgggatcg ccggtgacca
    10921 gcaggccgcg ctgtttggtc agttgtgcgt gaaagaaggg atggcgaaga acacctatgg
    10981 cactggctgc tttatgctga tgaacactgg cgagaaagcg gtgaaatcag aaaacggcct
    11041 gctgaccacc atcgcctgcg gcccgactgg cgaagtgaac tatgcgttgg aaggtgcggt
    11101 gtttatggca ggcgcatcca ttcagtggct gcgcgatgag atgaagttga ttaacgacgc
    11161 ctacgattcc gaatatttcg ccaccaaagt gcaaaacacc aatggtgtgt atgtggttcc
    11221 ggcatttacc gggctgggtg cgccgtactg ggacccgtat gcgcgcgggg cgattttcgg
    11281 tctgactcgt ggggtgaacg ctaaccacat tatacgcgcg acgctggagt ctattgctta
    11341 tcagacgcgt gacgtgctgg aagcgatgca ggccgactct ggtatccgtc tgcacgccct
    11401 gcgcgtggat ggtggcgcag tagcaaacaa tttcctgatg cagttccagt ccgatattct
    11461 cggcacccgc gttgagcgcc cggaagtgcg cgaagtcacc gcattgggtg cggcctatct
    11521 cgcaggcctg gcggttggct tctggcagaa cctcgacgag ctgcaagaga aagcggtgat
    11581 tgagcgcgag ttccgtccag gcatcgaaac cactgagcgt aattaccgtt acgcaggctg
    11641 gaaaaaagcg gtcaaacgcg cgatggcgtg ggaagaacac gacgagtaat gtaaatgccg
    11701 gatgaagcgt ttatgccgca tccggtagtc ctgaaacgtg cgggggcaac cccgcacaca
    11761 tcaataatcc cacccttccc ctgtgctaca cttcgcgcca ttccttactg cttagagttt
    11821 gctatgagac gagaacttgc catcgaattt tcccgcgtca ccgaatctgc ggcgctggct
    11881 ggctacaaat ggttaggacg cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc
    11941 atgcgtatta tgctcaacca ggtcaacatt gacggcacca tcgtcattgg tgaaggtgaa
    12001 atcgacgaag caccgatgct ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg
    12061 gtagatattg ctgttgatcc gattgaaggc acgcgcatga cggcgatggg ccaggctaac
    12121 gcgctggcgg tgctggcagt aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat
    12181 atggagaagc tgattgtcgg gccgggagcc aaaggcacca ttgatctgaa cctgccgctg
    12241 gcggataacc tgcgcaatgt agcggcggcg ctcggcaaac cgttgagcga actgacggta
    12301 acgattctag ctaaaccacg ccacgatgcc gttatcgctg aaatgcagca actcggcgta
    12361 cgcgtatttg ctattccgga tggcgacgtt gcggcctcaa ttctcacctg tatgccagac
    12421 agcgaagttg acgtgctgta cggtattggt ggcgcgccgg aaggcgtagt ttctgcggcg
    12481 gtgatccgcg cattagatgg cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa
    12541 ggcgacaacg aagagaatcg tcgcattggc aagcaggagc tggcacgctg caaagcgatg
    12601 ggcatcgaag ccggtaaagt attgcgcctg ggcgatatgg cgcgcagcga taacgtcatc
    12661 ttctctgcca ccggtattac caaaggcgat ctgctggaag gcattagccg caaaggcaat
    12721 atcgcgacta ccgaaacgct gctgatccgc ggcaagtcac gcaccattcg ccgcattcag
    12781 tccatccact atctggatcg caaagacccg gaaatgcagg tgcacatcct ctgattgatt
    12841 tgatcgattg agccttccag tccttcggga ctggaatttt tttgttcgga gaacgaagat
    12901 aaggcaagtc aatcaaaaca ggagaaaaac atggctgatt gggtaacagg caaagtcact
    12961 aaagtgcaga actggaccga cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg
    13021 tttaccgccg ggcaattcac caagcttggc cttgaaatcg acggcgaacg cgtccagcgc
    13081 gcctactcct atgttaactc gcccgataat cccgatctgg agttttacct ggtcaccgtc
    13141 cccgatggca aattaagccc acgattggcg gcactgaaac caggcgatga agtgcaggtg
    13201 gttagcgaag cagctggctt ctttgttctg gatgaagtac cagattgcga aacgctatgg
    13261 atgctggcaa ccggtacagc gattggccct tatttatcga ttctgcaact aggcaaagat
    13321 ttagatcgct tcaaaaatct ggtcctggtt cacgccgcac gttatgccgc cgacttaagc
    13381 tatttgccac tgatgcaaga actggaaaaa cgctacgaag gcaaactgcg cattcagacg
    13441 gtggtcagtc gggaaacggc agcggggtcg ctcaccggac ggataccggc attaattgaa
    13501 agtggggaac tggaaagcgc gattggcctg ccgatgaata aagaaaccag ccatgtgatg
    13561 ctgtgcggca atccacagat ggtgcacgat acacaacagt tgctgaaaga gacccggcag
    13621 atgacgaaac atttacgtcg ccgaccgggc catatgacag cggagcatta ctggtaagcg
    13681 gttacttatc gataaacggc acgatgagca aatccgcact catcttattg atcatcccgc
    13741 gatatgccgg catcaaacgg ttgataaatg agtgatgatg accacagaca aggaggtcgc
    13801 actgctcttt ttgcataatt tccagcagtg tttccggcat ttctccgcgt tcaatacgca
    13861 gttttgtctt cggccattga atatttttcg tcagtttata cagcttgtta tccgacttat
    13921 tcttcaacaa ttgaagaata tcttctgttg cagggaagta gatacccgga tacaactcgc
    13981 ttaagccatc atcaatatga attaacgtca ggtgagcgtc attatgtctg gcgagctcca
    14041 gagctttatt caccagtaag gcatcttctt cattcccgga agg
//
LOCUS       DAJTNA010000098        14067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTNA010000098 DAJTNA010000000
VERSION     DAJTNA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14067
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.099"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..401
                     /locus_tag="OO161_002505"
                     /pseudo
     CDS             1..401
                     /locus_tag="OO161_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="OO161_002506"
     CDS             484..741
                     /locus_tag="OO161_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463543.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO161_002507"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO161_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5463544.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO161_002508"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO161_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5463545.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO161_002509"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO161_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5463546.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO161_002510"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO161_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5463547.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO161_002511"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO161_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5463548.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO161_002512"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO161_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5463549.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO161_002513"
     CDS             complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO161_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5463550.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9142..9324
                     /locus_tag="OO161_002514"
                     /pseudo
     CDS             9142..9324
                     /locus_tag="OO161_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9318..9422
                     /locus_tag="OO161_002515"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="OO161_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="OO161_002516"
     CDS             9762..10019
                     /locus_tag="OO161_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5463551.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(10339..10470)
                     /locus_tag="OO161_002517"
                     /pseudo
     CDS             complement(10339..10470)
                     /locus_tag="OO161_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10752..12110
                     /locus_tag="OO161_002518"
     CDS             10752..12110
                     /locus_tag="OO161_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5463552.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(12349..13734)
                     /locus_tag="OO161_002519"
     CDS             complement(12349..13734)
                     /locus_tag="OO161_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5463553.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(13784..>14067)
                     /gene="tnpB"
                     /locus_tag="OO161_002520"
     CDS             complement(13784..>14067)
                     /gene="tnpB"
                     /locus_tag="OO161_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5463554.1"
                     /translation="FNGLAAKVQTALKDDPMSGHIFIFRGRSGSQVKLLWSTGDGLCL
                     LTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDWRQPKRLLTSLTML"
BASE COUNT         3271 a         3764 c         3862 g         3170 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaacgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agcaattgaa ccgccccggg tttcctggag
    10321 agtgttttat ctgtgaactc aggctgccag atcatcgttt ccgatggaag cataataagc
    10381 tttttctgct tctgccggtg ggatatggcc ctgcctttcc agcaatcgtc gattgttata
    10441 ccagtccacc cacgtgagtg tggtcagctc atcatactga cacgtgcatt acccctaccg
    10501 cgataatgca accgtacaat atagggcgtt tcctaaacgc agccctcatc actccaatcc
    10561 aacaagacac agcatttttg atcacgaaga aagacaaata atttccgtaa atgtttccgt
    10621 tgttacaaat aacaacagtg ttttacattt ctttggcctc aggcccgcgc atgttgccat
    10681 tcgatgtcat tttcctgact cgtttcttct ttatggtgta gtgtgatctt cactttatag
    10741 atgctaacaa tatgaaaaga aaaattattc ccgtgctaat cggttgtacg ctctctttct
    10801 ctgccctggc ggcacaacct acggctgagc gctatgtcgt cagttttcca gaaggcacgc
    10861 acgtgaacta cgccggcgca tttgccagtg cattcccgaa cggccttccg gttgggatag
    10921 gttctggcct gctgttcacc ggtaaacagg gcgatgccct gacattcgca accattaccg
    10981 atcgcggtcc gaacgcggat tcaccgaaag aggggaaaaa cgaaactaaa attttcgtta
    11041 cgccagattt cgcgccgctg ctgatgacga ttcgtgtaca aaacggcaaa gcggaggcca
    11101 tcgatccccg tcctctgcat gacgataaag gcgcgatcaa cggtctgccg ctggcaagcg
    11161 acgtgattgg ctccaccaat gaagtggcct tcagcgacac gttacataga ctgaaggggg
    11221 ataatcgcgg gctggatacg gaagggatca ctccggatgg taaaggaggt tactggctgt
    11281 gcgatgagta tggcccgttc ctgattaaca tcgacagtaa agggaaaatc ctggcaatcc
    11341 acggtccgca ggcggcggaa ggggaaaaag cgattgcggg cggtttgcca aatatcctca
    11401 aatggcgtca ggcgaaccgc ggctttgaag gcctcacccg tatgccggac gggcgtatta
    11461 ttgtcgccgt gcaaagtacg ctggatatcg acgctaagag caagaaaaag gcgctgttta
    11521 cgcgtctggt gagcttcgat ccggcaagcg gaaaaaccgc gatgtacggt tatcctatcg
    11581 acagcgcggc ctacagcaaa aacagcgacg ctaaaatcgg agacatcgtg gcgctcgata
    11641 accagcacat cctgctgatc gaacagggaa gagataaaaa caacagaatg cgtaacctga
    11701 tttacgaggt cgatctcaat aaggcgagcg acctgtcagg cttcgacaag ccgggtgagt
    11761 atccggagtt tgatgatgag aaaaccctgt cacagcgcgg cattaccctt gcgcagaaaa
    11821 cgcaggttgt tgatctgcgc tccttgggct ggcagcagga gaaggctgaa gggttggcgc
    11881 tgatcgacag caaaacgctg gcggtggcga acgataacga ctttggcgtg aaggtggcga
    11941 tgcaacatcc ggtcgagggc aagacattca aggattatcg ggtgaacgcg gaaggaaagc
    12001 tgacgcttga cgacaagcag gttgaaacga ccctcagagt gaagccactg gagaaaccgg
    12061 agtctgacag tgaactgtgg attgtgacgc tgccggaagc gttgaaataa gtttataagg
    12121 tacggacagc agtatcgctt agtttttcaa attcatgtat gtgaacaata ttggggttcc
    12181 ggcgtagacc cccaatgaaa gcgtcaccac gtgaaaacat gcctgcacgg tttacacgtc
    12241 atcccatggt ggcagccggt taactttcct gcgccaccag tggaaaaagg ccataagcag
    12301 tccgaacaaa agtaagcgta cagcgagggc cgtattgacg gggatgtgtt attcagctgg
    12361 cagtgctatg cgccacggaa gcagttcgct gacccggttg accggccagt ctgctatgac
    12421 gccaagcaca tggcgaaggt agctttctgg atccacgtca ttcagtttgc acgtcccgat
    12481 caggctgtac agtagcgctc cccgctcacc accatggcca gagccgaaga acagggtctt
    12541 catcttttca cgcaaccagc tttccaggga tttcaacagc ggtttcgttt ttcgctgacg
    12601 ttcagcaagc cgctgctctg ccggcattcc ccttatatcc gcctctatgg cgtacaactg
    12661 accgatctgc tccagggctt cttccgtcag tgctgacggg atgcggacgt gcacatcgtg
    12721 gatctttcgg cgggcatgag cccagcaggc agcttccgtt atcccaccat tgcgatacag
    12781 ctcgttgaac ccggcgtacg catccgcttg cagcacaccg ctgaagcaag caagatgagt
    12841 ctgcggatgg atgctttttc tgtccgggct gtaagcgaac cacactgcag gtgccaacgc
    12901 tgacccggca ttgcggtcat cacgaacata cgcccacaac cgcccggtct tcgtcttctt
    12961 attacccggc agcagtacct ggaccggggt atcatcggca tggagtttgc cgtcagtcat
    13021 gacatagcca tgaagcgcct cttccagcgg agacagcagc cggcagcatg catccaccca
    13081 gcccgacagc agtgaacggc tcagctccac accttgccgg ccgtatattt ctgactggca
    13141 atacagcggg gtgtgctctg catacttcga ggtcagcacg cgggccagca gccccggtcc
    13201 ggcgataccc cgctcgatgg gccgcgaagg tgcaggtgcc tgcacgatgg tatcgcactg
    13261 agtacaggca tgtttttccc gtaccgtccg gataacccgg aaggcgctac gcatcaactc
    13321 cagctgttcg gcggtatcct cgcccagata gctcagtgaa ccgccgcagt tcgggcagca
    13381 cggcgccgca ggcaacagtc gcttttcgtc acggggtagt gattcaggga acggcttacg
    13441 ggtgcgggtc tgacgcaacg gacgctgtac tgccgggtca tacaccctac cagtcagcgt
    13501 atcgctctct ttctgaagcc ggttcagatc ggcttccatt tgtgcgatac ggcgggagac
    13561 tttttcggaa cgactgccga agttcatccg gcggagttta tccagctgcg cctgcagatg
    13621 gtctatttcg cgctcccggt tgctcagctt ttcctgcagg gcgtggatca gcgcttcctg
    13681 ttcggccagg cgctgtttca gcaggaagat gtcgtcagaa gagatgtcgt tcataagccc
    13741 gtattttacc gggcttattc tgtgacaacc aggataaaga gatttacagc atggtcaggg
    13801 aggtcagcag ccgcttgggc tgtcgccagt cgataccttc cagcagcatc gccagctgcg
    13861 cctgcgtaag gaacactttg ccatcacggg ctgacggcca ggcgaagcgc ccacgctcca
    13921 gccgtttggt caggaggcac agtccgtcgc cggtggacca cagcagttta acctgactgc
    13981 cgctgcggcc ccggaaaatg aaaatatggc cggacatggg atcgtctttc agcgccgttt
    14041 gtactttcgc agccaggccg ttgaagc
//
LOCUS       DAJTNA010000099        14007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTNA010000099 DAJTNA010000000
VERSION     DAJTNA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14007
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.100"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..422
                     /locus_tag="OO161_002521"
     CDS             <1..422
                     /locus_tag="OO161_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5463555.1"
                     /translation="LMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRL
                     AEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFHLHNGHQTTMTL
                     SIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            438..920
                     /locus_tag="OO161_002522"
     CDS             438..920
                     /locus_tag="OO161_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5463556.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGITHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENLRVAVVITTP
                     "
     gene            complement(997..1344)
                     /gene="rplS"
                     /locus_tag="OO161_002523"
     CDS             complement(997..1344)
                     /gene="rplS"
                     /locus_tag="OO161_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5463557.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVYSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1386..2153)
                     /gene="trmD"
                     /locus_tag="OO161_002524"
     CDS             complement(1386..2153)
                     /gene="trmD"
                     /locus_tag="OO161_002524"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5463558.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2184..2732)
                     /gene="rimM"
                     /locus_tag="OO161_002525"
     CDS             complement(2184..2732)
                     /gene="rimM"
                     /locus_tag="OO161_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5463559.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2751..2999)
                     /gene="rpsP"
                     /locus_tag="OO161_002526"
     CDS             complement(2751..2999)
                     /gene="rpsP"
                     /locus_tag="OO161_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5463560.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3136..4497)
                     /gene="ffh"
                     /locus_tag="OO161_002527"
     CDS             complement(3136..4497)
                     /gene="ffh"
                     /locus_tag="OO161_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5463561.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4664..5455
                     /locus_tag="OO161_002528"
     CDS             4664..5455
                     /locus_tag="OO161_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5463562.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            5476..6762
                     /locus_tag="OO161_002529"
     CDS             5476..6762
                     /locus_tag="OO161_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5463563.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6817..7410)
                     /gene="grpE"
                     /locus_tag="OO161_002530"
     CDS             complement(6817..7410)
                     /gene="grpE"
                     /locus_tag="OO161_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5463564.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7533..8411
                     /gene="nadK"
                     /locus_tag="OO161_002531"
     CDS             7533..8411
                     /gene="nadK"
                     /locus_tag="OO161_002531"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5463565.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8497..10158
                     /gene="recN"
                     /locus_tag="OO161_002532"
     CDS             8497..10158
                     /gene="recN"
                     /locus_tag="OO161_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5463566.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10307..10648
                     /gene="bamE"
                     /locus_tag="OO161_002533"
     CDS             10307..10648
                     /gene="bamE"
                     /locus_tag="OO161_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5463567.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10710..11000)
                     /locus_tag="OO161_002534"
     CDS             complement(10710..11000)
                     /locus_tag="OO161_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5463568.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10990..11427)
                     /gene="ratA"
                     /locus_tag="OO161_002535"
     CDS             complement(10990..11427)
                     /gene="ratA"
                     /locus_tag="OO161_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5463569.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11598..12080
                     /gene="smpB"
                     /locus_tag="OO161_002536"
     CDS             11598..12080
                     /gene="smpB"
                     /locus_tag="OO161_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5463570.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIGEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12295..12657
                     /gene="ssrA"
                     /locus_tag="OO161_002537"
     tmRNA           12295..12657
                     /gene="ssrA"
                     /locus_tag="OO161_002537"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12818..14007)
                     /locus_tag="OO161_002538"
                     /pseudo
     CDS             complement(12818..14007)
                     /locus_tag="OO161_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         3459 a         3780 c         3319 g         3449 t
ORIGIN      
        1 cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt actatttctt gatggcgata
       61 atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagggtc ttgattgaaa
      121 tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc ctgggcggcg
      181 atgaattcgc tatggtgctc tatgatgtac agtcggaatc tgaagtgcaa cagatatgct
      241 cagcactgac acaaatcttt aatctcccgt ttcatcttca taatggccat cagaccacca
      301 tgacattaag cattggttac gcgatgacca ttgagcacgc ctctgcggaa aaattacaag
      361 agcttgccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag ctggtgagat
      421 aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc acctcactaa
      481 tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc gccgctatgc
      541 aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca gatgccattc
      601 tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc caaaccatgg
      661 cagccaaatt ggcctcgaca gggataacac atgcccgtat ggatggacac accgataact
      721 atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc gtggccgatg
      781 catgggctat tggtggacaa attccacgca gcaatctcac cacacagggt ttaggaaaaa
      841 aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaacctc cgcgtcgcag
      901 tggtgattac aaccccttaa aacacgagca aacagacaaa aaaagggcca gccaattggc
      961 cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct ctttgatacg
     1021 agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa cagcaccacg
     1081 acgtttgaca gaaatgctgt aaactaccgg agagtgagtc tggaagacac gctcaacgcc
     1141 ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt tacgaatagc
     1201 gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa cccatacttt
     1261 cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct gctcttgttc
     1321 aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac tgatatctcg
     1381 ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt tttgaactcc
     1441 gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc aggtcttcta
     1501 agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc atggttgccc
     1561 gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt atagtgcggg
     1621 caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg tcccagtacc
     1681 cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc accaccactg
     1741 agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac gcgctcatct
     1801 ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccaa ttcgctgacg
     1861 cccgcttgat caagcttgcg tccctgtggt gacagataaa tcacctttgc gccttcaccc
     1921 gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat taacatcccc
     1981 ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt gaagtcgcga
     2041 ggactccagc tctggatgct cagcaggcca tttttaactg cccggccagt taccccgtaa
     2101 tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca catagcgccg
     2161 tcttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc gattgaacga
     2221 gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag acgttccttg
     2281 ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattaga tccggtttcc
     2341 atcatatcga cgactttacc gagatcgtag ccttcagtgg ttactacctg gcagcccatc
     2401 aggtctttcc agtagtagtc accctcttca agctgaggca actgcgatga atccacgaca
     2461 atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt cagcttgatg
     2521 atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca ctgacccgcc
     2581 ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt ggaagaaaac
     2641 actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac gatgggatca
     2701 acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga ttaagctgct
     2761 ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc ctggccaacc
     2821 cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct agcgattggg
     2881 ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct gtctgcgaca
     2941 acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat agttaccata
     3001 acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc cggtgtcata
     3061 ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt taataactct
     3121 gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcatcccct tcatgcttct
     3181 catcatcttc gccattccgc ccttcttcat tttcttcatc atgcgctgca tgtcgtcgaa
     3241 ctgtttcaga agacggttaa cgtcctgcac ctgcataccg caaccggcag caatacggcg
     3301 tttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca tcgagttgat
     3361 gatggcttcc atacgcacca gcactttatc gtccatctgt gacttgacgt tatccgggat
     3421 ctgccccatg cccggcagct tgcccatcag gctggccatg ccgcccatat ttttcatctg
     3481 gcgcagctgc tcaagaaagt cgttgagatc gaagccgtca ccttttttca gcttgctggc
     3541 taatttctct gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca gcgacagtac
     3601 gtcgcccatg ccgagaatac gcgacgcaat gcggtccgga tggaacggct ccagcgcctc
     3661 agttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac gaatagagag
     3721 cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacgccgg taagcggtaa
     3781 cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg cgtcaaccac
     3841 aaacagggtt tcaaccgggt taatcgacgc atggacttgt ttgatctcgt ccatcatcgc
     3901 ttcgtcaacg tgcagacgac cagcggtatc caccagcagc acgtcgtaga atttcagttt
     3961 ggcttctttc agcgccgcgt taacgatatc taccggcttc tgaccaacat cagaagggaa
     4021 gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg ccgggcgata
     4081 aacgtcggca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga acttaccgag
     4141 tttaccaacg ctggttgttt taccggcacc ttgcaggccc gccatcagca cgaccgcagg
     4201 cggttgcgcc gccaggttca gggtctggtt ctcttcgccc atcgccgcaa ccagttcgtt
     4261 acggacgatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt catgaccaac
     4321 cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga catccgcctc
     4381 cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag ttaggcgtcc
     4441 acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat caaacattgt
     4501 ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt attttgccgc
     4561 agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt cacctgacgc
     4621 tatactgctt ctctttctta ttgctcaaac tgtcgacatc actatgcccg tttttgctct
     4681 gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc tgctgcaaaa
     4741 aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg tctgccacgc
     4801 aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc tcagcctgct
     4861 gaacgttggt tcattggtca gtttgatgat ctgtacggta atgaccattg tggcttctcg
     4921 caatcgtggc tggttgctgc tacccattgt ctatgccttt gcgcttatca acctggcgct
     4981 ggcgaccttc atgcccaatg aatacatcac ccatctggaa gcaacgcctg ggatgctggt
     5041 gcacattggc ttatcgctct tttcctatgc cacgctaatt atcgccgccc tgtacgcgct
     5101 gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcattta accaggaaat
     5161 gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg gcgtggtgct
     5221 gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacctattta gcatggaaaa
     5281 tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc tgctgtgggg
     5341 acattaccat gaaggatggc gtggacgccg cgtcgtctgg tttaacgttg cgggcgcggt
     5401 aattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca gctaaaccca
     5461 gaaaaggagt ttcccctgga acacatttct actactacgt tgatcattat tctgatcatc
     5521 atggtggtca tttcagccta tttttccggg tccgaaaccg gaatgatgac cctcaaccgc
     5581 tatcgtctgc gacatatggc gaaacagggt aatcgctcgg ccaaacgcgt cgaaaaattg
     5641 ctgcgtaagc cagaccgcct gataagcctg gtgttaatcg gcaataacct ggtcaatatt
     5701 cttgcctccg cgctcggcac tattgttggg atgcgtttgt acggcgatgc gggcgtggca
     5761 attgcgactg gtgtgctgac ttttgtcgta ctggtatttg ctgaggtatt gccgaaaacc
     5821 attgccgcgc tgtacccgga aaaagtcgct tatccgagta gttttctgct ggctccgctg
     5881 caaattttga tgatgccgct ggtctggttg ctgaatgcta tcacccgtat gctgatgcgc
     5941 atgatgggta tcaaaaccga tatcgtggtt agcggctctt tgagcaaaga agagttgcgc
     6001 actatcgtgc acgaatcacg ctcacaaatt tcccgtcgca atcaggatat gctgctgtcg
     6061 gtgctcgatc tggaaaaaat gaccgttgat gacatcatgg tgccgcgcag tgaaattatc
     6121 ggtattgata tcaacgatga ctggaaatcg attctgcgcc aactctccca ctcacctcac
     6181 gggcgcatcg tgctctaccg tgattcgctg gacgacgcca tcagtatgct gcgagtacgt
     6241 gaagcctggc ggttgatgtc ggagaaaaaa gagttcacca aagaaaccat gctgcgcgcc
     6301 gcggacgaga tctattttgt gccggaaggt acgccgctca gcacgcagtt ggtaaagttt
     6361 cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt atggagacat tcaggggctg
     6421 gtgacggttg aagatattct ggaagagatt gtcggcgatt tcactacgtc gatgtcgcca
     6481 acacttgccg aagaggtcac gccgcaaaac gacggttcgg taattatcga tggcaccgcc
     6541 aacgtgcggg aaatcaacaa agcctttaac tggcatctac cggaagatga tgcccgcacg
     6601 gttaatggcg tcattcttga ggcactggaa gagatccctg tcgcaggcac ccgcgtgcgt
     6661 attggcgagt acgatataga tattctcgac gtacaggaca atatgattaa gcaggtaaaa
     6721 gtttttcctg tgaaaccgct gcgcgagagt gtggcggagt aacgaaaacg gccctgcatt
     6781 cgcatgcagg gccgtgaatt attacgaaaa cagaaattac gcttttgctt tcgctacagt
     6841 taccatcgcc gcacgaatcg tacgaccatt cagcgtataa cccttctgca taatgcccag
     6901 tacgttacct ggcgcaacgt catcagattc caccattgcg atagcctgat gcacattcgg
     6961 gtccagcggc acgttagttt cggcgatcac ttcaacacca aacttacgca caacatccag
     7021 catcgacttc agcgtcagct caatgccttc aaccatcgca gacatatccg ggttagcttt
     7081 atcagccact tccagcgcac gatccaggct atcaatcacc ggcagcaatt cgttgatgaa
     7141 tttctccagc gcgaatttgt gggctttttc aatatccagt tcagtacgtc gacgcaggtt
     7201 ttccatttcg gcttttacac gcaaaatgcc gtcacgttca cgggtctggg cttcagccag
     7261 ctgagcttcg agattcgcaa ttttttcatc gcgcggatcc acctgctcag cagaagcttc
     7321 tggctcaact gcctcaatct cttcgtgctg atccatgata atttcttccg gggcttgccc
     7381 ctcaggcgtt ttctgttctt tactactcat gaatttctcc gcgttttttt cgcattcatc
     7441 tcgctaactt cgcttattat ggggatcagt ttcagggttt caagggaagc actcacattg
     7501 tcatcaatct tcgcaacaag gacctcggaa aaatgaataa tcatttcaag tgtattggca
     7561 ttgtgggaca cccacggcac cccactgcac tgacaacaca tgaaatgctc taccgctggc
     7621 tgtgcacaaa aggttacgag gtcatcgttg agcaacaaat cgctcacgaa ctgcaactga
     7681 agaatgtgaa aactggcacg ctcgcggaga ttgggcaact agctgatctc gcggtagtcg
     7741 ttggtggcga cggtaatatg ctgggcgcgg cacgcacact cgcccgttac gatattaaag
     7801 ttattggaat caaccgtggc aacctgggtt tcctgactga ccttgacccc gataacgccc
     7861 agcaacagtt agccgatgtg ctggaaggcc actacatcag cgagaaacgt tttttgctgg
     7921 aagcgcaagt ctgtcagcaa gattgccaga aacgcatcag caccgcgata aatgaagtgg
     7981 tgcttcatcc aggcaaagtg gcacatatga ttgagttcga agtgtatatc gacgagatct
     8041 ttgcgttttc tcagcgatct gatggactaa ttatttcgac gccaacaggc tccaccgcct
     8101 attccctctc tgcaggcggt cctattctga ccccctctct ggatgcgatt accctggtgc
     8161 ccatgttccc gcatacgttg tcagcacgac cactggtcat aaacagcagc agcacgatcc
     8221 gtctgcgttt ttcgcatcgc cgtaacgacc tggaaatcag ttgcgacagc cagatagcac
     8281 tgccgattca ggaaggtgaa gatgtcctga ttcgtcgctg tgattaccat ctgaatctga
     8341 ttcatccgaa agattacagt tatttcaaca cattaagcac caagctcggc tggtcaaaaa
     8401 aattattcta attttacgcc agcctcttta ctgtatataa aaccagttta tactgtacac
     8461 aataacagta atggtttttc atacaggaaa acgactatgt tggcacaact gaccatcagc
     8521 aactttgcta tcgttcgtga gcttgagatt gattttcata gcggcatgac cgtaataact
     8581 ggcgagaccg gcgcgggtaa atctattgca atagatgccc tcggtctttg tctcggtggt
     8641 cgcgctgaag ccgacatggt gcgtaccggc gctgctcgcg ctgacctgtg cgcccgtttt
     8701 tctctgaaag atacgccagc ggctctgcgc tggctggaag aaaatcagct tgaagacggg
     8761 catgaatgtt tgcttcgtcg cgtgatcagc agcgatggtc gctcccgtgg tttcatcaac
     8821 ggtacagctg ttcctctgtc acaactgcgc gaactgggtc agttgctgat tcagatccat
     8881 ggtcagcacg ctcatcaatt actcaccaaa cctgagcacc aaaaattcct gcttgatggc
     8941 tatgccaatg aaacctctct actgcaggaa atgaccgcac gttatcagtt gtggcatcaa
     9001 agctgccgtg acctcgcgca tcatcaacag ttaagtcagg aacgcgccgc ccgtgcggaa
     9061 ctgctgcaat accaattaaa agaacttaac gaatttaatc cgcagcccgg agagtttgaa
     9121 caaatcgacg aagagtacaa acgtctggcg aacagcggtc aattgctgac caccagccag
     9181 aatgcattgg cattaatggc ctacggtgaa gacgcaaacc tgcaaagtca gctttacacg
     9241 gctaaacaac tggtgagcga attgattggc atggacagca aactgtccgg cgtacttgat
     9301 atgctggaag aagctaccat ccagattgct gaagccagcg atgaactgcg ccactactgc
     9361 gatcgtctgg atctcgatcc caaccgacta tttgaacttg aacagcgcat ctcaaaacag
     9421 atttcgctgg cacgtaaaca tcacgtcagc cctgaggcat tgccacagta ttaccagtcg
     9481 ctactggaag aacagcagca actggacgat caggccgact cacaagaaac gcttgcgctg
     9541 gcggtaacga aacatcatca gcaggcactg gaaatcgcgc gcgcattaca ccaacaacgc
     9601 cagcaatatg cagaagaact tgcacagctg atcaccgaca gtatgcatgc gctctcaatg
     9661 ccgcatgggc agtttacgat cgatgttaaa tttgacgagc atcacctggg cgctgacggt
     9721 gccgatcgta ttgagtttcg ggtaaccacc aacccaggtc agccaatgca gcctattgcc
     9781 aaagtcgcat ccggtggtga attgtcccgc atcgcactgg caacccaggt catcacggcg
     9841 cgtaaaatgg aaaccccggc actgattttt gatgaagtgg atgtagggat tagcggtcca
     9901 acagcggcag ttgtcggcaa actgctgcgt caactcggcg aatcaactca ggtgatgtgt
     9961 gttacccacc tgccacaagt cgcgggatgt ggtcatcaac actattttgt cagcaaagaa
    10021 accgatggtg cgatgacaga aacgcatatg caatccctga ataaaaaagc gcggttacaa
    10081 gagctggcgc gcctgcttgg tggcagtgaa gtcacacgta atacactggc gaatgcgaaa
    10141 gaactgcttg cagcgtaaac ttttttcctg cttcacggtc agagtaaaca gcaaaacgcc
    10201 gtaagaccgg aaagcaaaag gttttaaagt gatgaaaggt ctattatcat cggcatatta
    10261 cagatgagcc acgtactgct cgggcccgaa aaggaatcaa atcactatgc gctgtaaaac
    10321 gctgactgct gcagcagcag tactattgat gttgaccgca ggctgttcca ctctggagcg
    10381 agtggtttac cgtcctgaca tcaaccaggg gaactatctg accgctaacg acgtatccaa
    10441 aatacgtgtt ggcatgacgc aacaacaagt tgcgtacgca ttgggtacac cgctgatgtc
    10501 cgatccattt ggtacgaata cctggttcta tgtcttccgc cagcaaccag gtcatgaagg
    10561 tgtaacgcag caaacgctga cgctgacctt taacagtagc ggtgtgttga ccaatattga
    10621 taacaaacct gcgctgagtg gtaactaata ataaagttgc tctcaaagac gttaaaaaag
    10681 gtgctcaatg agcacctttt ttctgtctgt tatttattcg ctgatttttc tgctcgttgc
    10741 ctgcgaagct ctttcggatc ggcaatgaga gggcgataaa tctccacccg atcgccatca
    10801 tgcacactat cgcttagttt tgccggacgg ctgtaaatgc cgactttgtt tttagttaaa
    10861 tcgatatcgg tacgcaattc cagcaagcca ctggcgcgaa tagcttcttc aaccgtcgcg
    10921 ccctcctgca gcgtcacgcg ctgcaggtac tgcttctcag gtagcgcata agccacctca
    10981 acggcaattt tacctggcac tgtaaacctc tttcgcacga accgtaaaag cctggaccat
    11041 attagacgcc agctctttaa acacgcgacc aaaggcgagt tcaatcaact tattggtaaa
    11101 ctcaaagtcg agatgaaact cgatacgaca cgcctcctgg ctcagcggcg taaacttcca
    11161 tccaccaatc aatttcttga acggcccatc caccagattc ataagaatac tttggttact
    11221 ggtcaactgg ttgcgggtag taaacgtttt gctgatccca gccttagaga catctaccgc
    11281 cgcagtcatc tgcccaggag tggactccag aatccgactt ccggtacaac ccggcaaaaa
    11341 ctgaggataa gactgaacgt cattcactaa ctgatacatt tgctccgcgc tgtagggtac
    11401 cagtgcggtc cggctaatct gaggcataac tatttccatc aacaaaaatc caacaaataa
    11461 tatcatttat cctgctgtaa aaaaaacgct atcccggcgc tgggtaacat cgggttcatg
    11521 ctaagataga gccttgtccc ccgcaggatt gatatggggt gttttcgatt tcagattacc
    11581 gatgattcac gacgcttatg acgaagaaaa aagcacataa acctggttca gcgaccatcg
    11641 cgcttaacaa gcgcgcccgt cacgaatact ttatcggaga agagttcgaa gcgggacttg
    11701 ccctgcaagg ctgggaagtt aaatccctgc gcgcaggaaa agccaatatc agcgacagct
    11761 acgtccttct gcgtgacgga gaggcatttc tgtttggcgc taacatcacg ccaatggccg
    11821 tggcctccac gcatgtggtg tgcgatccta cccgtacccg caagttactt ctcaaccagc
    11881 gcgaactgga ctcattgtac ggtcgcgtca atcgagaagg ctataccgta gtggcgctct
    11941 ccctgtactg gaaaaatgcc tggtgcaaag tgaaaatcgg cgtcgccaaa ggtaaaaaac
    12001 agcacgataa acgttcagat atcaaagaac gcgaatggca ggtggataaa gcacgtatca
    12061 tgaaaaacgc ccaccgttaa acctgcactc caattattga ccagttcctc accgcgcctc
    12121 cctctccggc ggcgcgaatg aacatcttat tggctatcac atccgacaca aatgttgcca
    12181 tcccattgct taatcgaata aaaatcaggc tacatgggtg ctaaatcttt aacgataacg
    12241 ccattgaggc tggtcatggc gctcataaat ctggtatact tgccgttaca cattggggct
    12301 gattctggat tcgacgggat ttgcgaaacc caaggtgcat gccgaggggc ggttggcctc
    12361 gtaaaaagcc gcaaaaaata gtcgcaaacg acgaaaacta cgctttagca gcttaataac
    12421 ctgcttagag ccctctctcc ctagcctccg ctcttaggac ggggatcaag agaggtcaaa
    12481 cccaaaagag atcgcgtgga agccctgcct ggggttgaag cgttaaaact taatcaggct
    12541 agtttgttag tggcgtgtcc gtccgcagct ggcaagcgaa tgtaaagact gactaagcat
    12601 gtagtaccga ggacgtagga atttcggacg cgggttcaac tcccgccagc tccaccaatc
    12661 atgattggac ggtataagga caacaccaat aaaaacagga agttagcagt ctcagcagga
    12721 caccgaccag acggtgaaga gacataaaag gatacgcaaa ggagccgcgg ctcctggtga
    12781 catgaaagcc cacagatgtg ggctttttcg ttgatggtca gaacgaccag ttcacaccag
    12841 ctaccccgtt ccacggggat tccacaccgg caccatggct ataccccacc ccaagatgcc
    12901 cgcttaacct actgctgaat gaggctttaa tacctgcctg gtatattcca cgtctgcccg
    12961 acaaatcatt gacgaaatta ccgtcactat tcactttcac ccggttatca tcgacaaatt
    13021 ctttgcgcac agccgccttc agccacggct caacttccat accgttcccc agacgcatgt
    13081 tgtaactcag cgttgcaccc agttcacgat ataaactgcg ggtatcgact gatttcgatt
    13141 ccatgccatt ggataaatga tattcagggt tatcagcggt gaacccccgt taacgatgca
    13201 tacggcgtca ggttccagtt accatcggta aatcgcatcc cggtttcaat gtgaccgccc
    13261 agcccgttgc tgcgataact gccgttggcg gctccaccgc tgccaaccgt ggagtcgcga
    13321 gcttcggtac ctgctaatac caataaatta ccgccatttt ccagcaacat attggtcgct
    13381 aaattgccgg aaatggaaaa agtgccgtac tggtgagtac cgctgatttc aataccgtta
    13441 gccgtgctcg tctggagagc ggcaccgctg ttctggacgg tatctgtcgc tttgccatta
    13501 tcgttaactg tcagcgtacc gccttcattg atctttgttt ttattgcctc tccgttagct
    13561 gaaactgttt gtattccgcc gtcgttaatc gttgtctcat tcgccacccc ctcgacgatt
    13621 tgttcgccgc cggtgagcgt cgtgcctgtc gcagtggctt ttgttttgac gatctcccgt
    13681 ccgcccgtat tgacctgtgt tttgtcagaa gaggtgtctg actccacggt taacacgccg
    13741 ccatttgcca gcaggatatt gttcgccgca ccctgctcga tgctgaacgc gacgccatcc
    13801 gcgcgtgttc ctgtgacccg cgtcgccctg gtagttgcaa ccaaagcgcc ctggctactc
    13861 tgctgtatcc ccgttgcgct gcctttctcc cgcacatcga gtgtgccgcc gtcattaagc
    13921 accgagtttt cagccagaca gccctcatta actacctgtg ccccccccat taataatgga
    13981 accttccgct gtcccgtttg ccataat
//
LOCUS       DAJTNA010000100        13963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTNA010000100 DAJTNA010000000
VERSION     DAJTNA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.101"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            141..185
                     /locus_tag="OO161_002539"
     CDS             141..185
                     /locus_tag="OO161_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463571.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            438..758
                     /gene="iraM"
                     /locus_tag="OO161_002540"
     CDS             438..758
                     /gene="iraM"
                     /locus_tag="OO161_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5463572.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKLGIILQEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     SICY"
     gene            complement(826..2076)
                     /gene="icd"
                     /locus_tag="OO161_002541"
     CDS             complement(826..2076)
                     /gene="icd"
                     /locus_tag="OO161_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5463573.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVTIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            2248..2901
                     /gene="rluE"
                     /locus_tag="OO161_002542"
     CDS             2248..2901
                     /gene="rluE"
                     /locus_tag="OO161_002542"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5463574.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2911..3372
                     /gene="nudJ"
                     /locus_tag="OO161_002543"
     CDS             2911..3372
                     /gene="nudJ"
                     /locus_tag="OO161_002543"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5463575.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3426..4532
                     /gene="mnmA"
                     /locus_tag="OO161_002544"
     CDS             3426..4532
                     /gene="mnmA"
                     /locus_tag="OO161_002544"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5463576.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4568..5209
                     /gene="hflD"
                     /locus_tag="OO161_002545"
     CDS             4568..5209
                     /gene="hflD"
                     /locus_tag="OO161_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5463577.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            5213..6583
                     /gene="purB"
                     /locus_tag="OO161_002546"
     CDS             5213..6583
                     /gene="purB"
                     /locus_tag="OO161_002546"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5463578.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVPNFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6751..7422
                     /gene="phoP"
                     /locus_tag="OO161_002547"
     CDS             6751..7422
                     /gene="phoP"
                     /locus_tag="OO161_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5463579.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7422..8882
                     /gene="phoQ"
                     /locus_tag="OO161_002548"
     CDS             7422..8882
                     /gene="phoQ"
                     /locus_tag="OO161_002548"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5463580.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8958..10079
                     /gene="roxA"
                     /locus_tag="OO161_002549"
                     /gene_synonym="ycfD"
     CDS             8958..10079
                     /gene="roxA"
                     /locus_tag="OO161_002549"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5463581.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGERLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(10128..11354)
                     /gene="pepT"
                     /locus_tag="OO161_002550"
     CDS             complement(10128..11354)
                     /gene="pepT"
                     /locus_tag="OO161_002550"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5463582.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLAGMKGTVERADMH
                     YIIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11604..12740
                     /gene="potA"
                     /locus_tag="OO161_002551"
     CDS             11604..12740
                     /gene="potA"
                     /locus_tag="OO161_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5463583.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12724..13587
                     /gene="potB"
                     /locus_tag="OO161_002552"
     CDS             12724..13587
                     /gene="potB"
                     /locus_tag="OO161_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5463584.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(13819..13890)
                     /locus_tag="OO161_002553"
                     /pseudo
     CDS             complement(13819..13890)
                     /locus_tag="OO161_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         3489 a         3484 c         3440 g         3550 t
ORIGIN      
        1 gttttggttt agtcatcatc cagttatatg tattttagcc aggaacaggt taaacctttc
       61 ctatataact caaaaattga aaccttattc tcatgtcatg tttatattca ttattatcgt
      121 tatataaaaa ggcaaccata atgtttagca aattggcacc tagtagcata aaggctatgt
      181 tttaattaca ggatgttcag tcatttgaat gtataacatt atagctaaac aaatctaaaa
      241 cgaagtgaat aatttattgt tttcactaaa tctcattttg tttaacatcc attgagattc
      301 cttgctttaa attttgtttt atataagcaa tcgttttaat taatttattt tttgaagggg
      361 taatatactc atatgcaaaa taaagaaatg aacatccaaa tgaactatat taaataccgt
      421 gggataagac ataacaaatg aagtgggtag taattgacac ggtaattcaa cctacatgcg
      481 gcatatcttt ttcagccata tggggtgata tgaaaatgat catctggtat caatctacta
      541 tatttcttcc tcctggaagt atatttacac cggttaagct tggtattatc cttcaggaaa
      601 aagaatatcc tattactatt tataacattg caccattcaa caagaattta tggagtttac
      661 tcaaaagcag tcaagagtgt cctccaggag aaagcaaaat aacaaataaa tgtttacata
      721 atagttgcat tataaaaata tgctcgatat gctattaata atatccacat aaataaaaca
      781 acgggcgtgt tatacgcccg tttcaatatt taactcatgg atagattaca tgttttcgat
      841 gatcgcttca ccaaactctg aacatttcag cagcttagcg ccttccatca gacgttcaaa
      901 gtcataagtt acggttttcg cgttgatagc accttccata cctttaacaa ttaaatcagc
      961 cgcttcagtc caccccatat ggcgcagcat catctcagcg gagaggatga tagagcctgg
     1021 atttactttg tcctgaccgg cgtatttcgg cgcagtaccg tgggtggctt caaacagggc
     1081 gcattcgtca ccgatgtttg ctccaggggc gataccgata ccgccaacct gcgctgccag
     1141 ggcgtcagaa atgtagtcac cgttcaggtt catacaggcg ataacatcat attcagccgg
     1201 acgcagcagg atctgttgca ggaatgcatc agcaatcacg tctttaatga cgatctcttt
     1261 gccggtgttc gggtttttaa ctttcagcca cgggccgccg tcgatcagtt caccgccaaa
     1321 ctcttcacgc gccagctggt agccccagtc tttaaacgcg ccttcggtga acttcatgat
     1381 gttgcctttg tgcaccagag tcacagagtc acgatcgtta gcaattgcgt attcgatcgc
     1441 tgcacgaacc agacgtttgg tgccttcttc cgaacacggc ttaataccga taccacaatg
     1501 ttccgggaag cgaattttct tcacccccat ctcttcacgc aggaatttaa tcactttctc
     1561 ggcgtcggca gagtctgctt tccattcgat acccgcataa atgtcttccg agttttcacg
     1621 gaagataacc atatcggtca gttcagggtg tttaaccggg cttggagtgc cctgatagta
     1681 acgtaccgga cgcaggcaga tgtagagatc cagttcctgg cgcagggcaa cgttcagaga
     1741 gcgaataccg ccaccaacag gagtggtcag cggaccttta atggtaacgc gatattcacg
     1801 aatcagatca agggtttcag caggcagcca gacatcctga ccataaacct gtgtggattt
     1861 ttcaccggtg taaatttcca tccaggagat tttacgctcg cctttatagg ctttctcgac
     1921 tgcagcgtcg accactttca gcatggctgg ggttacatct acaccgattc catcaccttc
     1981 aatgtaaggg ataatcggat tttcaggaac gttgagtttg ccgttttgca gggtgatctt
     2041 cttgccttgt gccggaacaa ctactttact ttccattcac ctctccttcg agcgctactg
     2101 gtttgctcgt ctgccaccac gttgcatatg cgtttgcgtc ctgcgatacg gatgctttag
     2161 agcaattttt tgttaatgat ttgtaattgg cttgtcaata ctaccctatt gtttgccgtc
     2221 atgaaagatg cgcgttatta ggctataatg cggcaattca taatctctga aaataccatg
     2281 caaaaaactt cttttagaaa tcacctggtt aagcgattca gctcgcaacg ttctaccagg
     2341 cgtaaacctg aaaaccagcc cacgcgtgtg atcctgttca ataaacccta cgatgttctt
     2401 ccgcagttca ccgatgaagc cggacgcaaa acattaaaag aattcatccc ggttcagggc
     2461 gtttatgcag caggtcgtct tgaccgcgat agcgaagggt tactggtgct gaccaataac
     2521 ggcgcgttgc aggcgcgttt aacccagccg ggcaaacgca ccgggaaaat ctattatgtg
     2581 caggtggaag gtattcccac acaagacgca cttgaagcct tgcgcaatgg cgtaacctta
     2641 aatgatggcc ctaccctgcc cgccggcgcg gagctggttg acgaaccagc gtggttatgg
     2701 ccgcggaatc caccaattcg tgaacgcaaa agtattccca ccagctggct gaagatcacc
     2761 ttatatgaag gacgtaatcg ccaggtgcgc cgcatgaccg cccatgttgg tttccccacg
     2821 ctgcgactga ttcgttatgc gatgggtgat tacactttgg ataaccttgc caatggcgaa
     2881 tggcgagaag caacagaata aggatgcaca atgtttaaac cgcacgttac cgttgcttgc
     2941 gtagtgcacg cagagggtaa atttttagtc gttgaagaga cgattaatgg taaagcgtta
     3001 tggaaccaac ctgccgggca tctggaagcc gatgaaacct tagtggaagc tgccgcccgt
     3061 gagctatggg aagaaaccgg catcagcgcg caaccgcaac actttattcg tatgcatcag
     3121 tggattgcgc cagataaaac gccgtttttg cgtttcctct ttgccattga gcttgagcaa
     3181 atatgcccga cccagcctca tgacagcgat atcgactgct gtcgttgggt cagcgccgaa
     3241 gaaattttaa aggcgtcaaa tcttcgttcg ccgctggtgg cggaaagtat ccgttgttat
     3301 caaagcgggc aacgctatcc gctggagatg attggcgatt ttaactggcc ttttacaaag
     3361 ggtgtcatct aaaaggctgc gtgatagaat acgccgcctt gaagttcaat gtcgtgagtg
     3421 atccaatgtc tgaaaccgca aaaaaagtaa tcgtcggcat gtccggcggt gtcgattcct
     3481 ccgtttctgc ctggctgttg caacaacagg gatatcaggt tgaaggcctg tttatgaaga
     3541 actgggaaga agacgacggt gaggaatatt gcacagcggc agcggatctg gctgatgccc
     3601 aggctgtctg cgacaagctc ggcattgaac tgcacaccgt taactttgct gccgagtact
     3661 gggacaacgt cttcgaactg ttccttgccg aatataaagc cggtcgcacg ccgaatccgg
     3721 atattctgtg caacaaagag atcaaattta aagctttcct cgaatttgcc gccgaagatt
     3781 taggtgccga ttatatcgct accggtcatt acgtacgtcg ggccgatgtc gatggcaaga
     3841 gccgcctgct gcgtggtctg gacagcaata aagaccagag ctacttcctt tatacgctca
     3901 gccatgagca gattgcgcaa agcctgttcc cggtcggcga actggaaaaa ccgcaggtgc
     3961 gtaagattgc tgaagatctt ggtctggtca ccgcgaagaa aaaagactct accggcattt
     4021 gcttcattgg cgaacgtaaa ttccgcgagt tcctgggccg ttatctcccg gcgcaaccgg
     4081 gcaaaatcat taccgtcgat ggcgatgaaa ttggtgagca ccaggggctg atgtatcaca
     4141 ctctcggtca gcgtaaaggt ctgggtatcg gtggcaccaa agaaggcacc gaagaaccgt
     4201 ggtatgtggt ggacaaagac gtcgaaaaca acattctggt tgttgcgcag ggccatgaac
     4261 acccgcggct gatgtctgtc gggttgattg cccagcagtt gcactgggtc gatcgcgaac
     4321 cattcaccgg tactatgcgt tgcacggtaa aaacccgcta tcgccagacc gatatccctt
     4381 gcaccgtcaa ggcgctggac gatgatcgca ttgaagtgat tttcgatgaa ccggttgccg
     4441 ccgtgacgcc gggccagtct gccgtcttct ataacggtga agtgtgcctc ggtggcggta
     4501 ttattgagca gcgtctgccg ctgccggtct gattattatc tttacttaaa acaaggaagc
     4561 agtgaacgtg gcaaagaatt actatgacat caccctcgcc ctggccggta tttgtcagtc
     4621 ggcacgcctg gtgcaacaac tcgctcacca ggggcattgt gatgccgatg cgctacacgt
     4681 ctcactcaac agtattattg atatgaaccc cagctcgacg ctggcggttt ttggcggtag
     4741 cgaagccaac ctgcgcgtcg ggctggaaac cctgctcggc gtgctcaatg ccagcagtcg
     4801 ccagggctta aacgccgaat taacccgcta cacactcagc ttgatggtgc ttgagcgcaa
     4861 actctcctca gcgaaaggcg cgctcgacac tctgggcaac cggatcaacg gcctgcaacg
     4921 ccagctcgaa cacttcgatt tacagtccga aacgctgatg agcgcgatgg ctgctatcta
     4981 tgttgatgtg atcagcccgc ttggcccgcg cattcaggtc accggttccc ctgctgtact
     5041 gcaaagccca caagtgccgg cgaaagttcg cgcaaccctg ctggcaggca ttcgcgccgc
     5101 cgtgctctgg caccaggtcg gcggcggacg tctgcaactg atgttttctc gtaatcgcct
     5161 gaccactcag gcaaaacaaa ttcttgctca tttaaccccg gagttgtgat ctatggaatt
     5221 atcctcactg accgccgttt cccctgtcga tggacgctac ggcgataaag tcagcgcgct
     5281 gcgcgggatt ttcagcgaat atggtttgct gaaattccgt gtacaagttg aagtacgttg
     5341 gctgcaaaaa ctggccgcgc acgcagcgat caaggaagtt cctgcttttg ctgccgacgc
     5401 aatcggttac cttgatgcaa ttgtccccaa tttcagcgaa gaagatgccg cacgcatcaa
     5461 aaccatcgag cgtaccacta accacgacgt taaagcggtt gagtatttcc tgaaagaaaa
     5521 agtggcggag atcccggaac tgcacgcggt ttctgaattc atccactttg cctgtacttc
     5581 ggaagatatc aataacctct cccacgcatt aatgctgaaa accgcgcgtg atgaagtgat
     5641 cctgccgtac tggcgtcaac tgattgatgg cattaaagat ctcgccgttc agtaccgcga
     5701 tatcccgctg ctgtctcgta cccacggtca gccagccacg ccgtcaacca tcggtaaaga
     5761 gatggcaaac gtcgcctacc gtatggagcg ccagtaccgc cagcttaacc aggtggagat
     5821 cctcggcaaa atcaacggcg cggtcggtaa ctataacgcc cacatcgccg cttacccgga
     5881 agttgactgg catcagttca gcgaagagtt cgtcacctcg ctgggtattc agtggaaccc
     5941 gtacactacc cagattgaac cgcacgacta cattgccgaa ctgtttgatt gcgttgcgcg
     6001 cttcaacacc attctgatcg actttgaccg tgacgtctgg ggttatatcg cccttaacca
     6061 cttcaaacag aaaaccattg ctggtgagat tggttcttcc accatgccgc ataaagttaa
     6121 cccgatcgac ttcgagaact ccgaagggaa tctgggcctt tccaacgcgg tattacagca
     6181 tctggcaagc aaactgccgg tttcccgctg gcagcgtgac ctgaccgact ccaccgtgct
     6241 gcgtaacctc ggcgtgggta tcggttatgc gctgattgcg tatcaatcca ccctgaaagg
     6301 cgtgagcaaa ctggaagtga accgtgacca tctgctggat gagctggatc acaactggga
     6361 agtgctggcg gagccaatcc agacagttat gcgtcgctat ggcatcgaaa aaccgtacga
     6421 gaagctgaaa gagctgactc gtggtaagcg cgttgacgcc gaaggcatga agcagtttat
     6481 cgacggtctg gcgttgccag aagaagagaa agcccgcctg aaagcgatga cgccggcaaa
     6541 ctacattggt cgcgccatca ccatggttga tgagctgaaa taaactcgta tcagtgccgg
     6601 atggcgatgc tgtccggcct gcttattaag attatccgct ttttattttt tcactttacc
     6661 tcccctcccc gctggtttat ttaatgttta cccccataac cacataatcg cgttacacta
     6721 ttttaataat taagacaggg agaaataaaa atgcgcgtac tggttgttga agacaatgcg
     6781 ttgttacgtc accaccttaa agttcagatt caggatgctg gtcatcaggt cgatgatgca
     6841 gaagatgcca aagaagccga ttattatctc aatgaacatt taccggatat tgcgattgtc
     6901 gatctcggat tgccagacga ggacggtctg tcactgattc gccgctggcg tagcaatgat
     6961 gtttcactgc cgattctggt attaaccgcc cgtgaaagct ggcaggacaa agtcgaagta
     7021 ttaagtgctg gtgctgatga ttacgtgact aaaccatttc atattgaaga ggtgatggcg
     7081 cgaatgcagg cattaatgcg gcgtaatagc ggtctggctt cacaggtcat ttcgctacca
     7141 ccgtttcagg ttgatctctc tcgccgtgaa ttatctatta atgacgaagt gatcaaactg
     7201 accgcgttcg aatacaccat tatggaaacg ttgatacgca ataatggcaa agtggtgagc
     7261 aaagattcgt taatgctcca actctatccg gatgcggagc tgcgggaaag ccataccatt
     7321 gatgtactga tgggacgtct gcgcaaaaaa attcaggcac aatatccgca agaagtgatt
     7381 actaccgttc gcggccaggg ctatttgttc gaattgcgct gatgaaaaaa ttactgcgtc
     7441 tttttttccc gctctcgctg cgggtacgtt ttctgttggc aacggctgcg gtagtactgg
     7501 tgctttcgct tgcctacgga atggtcgcgc tgatcggtta tagcgtcagt ttcgataaaa
     7561 ctacgtttcg gctgttacgt ggcgagagca atctgttcta tacccttgcg aagtgggaaa
     7621 acaataagtt gcatgtcgag ttacccgaaa atatcgacaa gcaaagcccc accatgacgc
     7681 taatttatga tgagaacggg cagcttttat gggcgcaacg tgacgtgccc tggctgatga
     7741 agatgatcca gcctgactgg ctgaaatcga atggttttca tgaaattgaa gcggatgtta
     7801 acgataccag cctcttgctg agtggagatc attcgataca gcaacagttg caggaagtgc
     7861 gggaagatga tgacgacgcg gagatgaccc actcggtggc agtaaacgtc tacccggcaa
     7921 catcgcggat gccaaaatta accattgtgg tggtggatac cattccggtg gagctaaaaa
     7981 gttcctatat ggtctggagc tggtttatct atgtgctctc agccaatctg ctgttagtga
     8041 tcccgctgct gtgggtcgcc gcctggtgga gtttacgccc catcgaagcc ctggcaaaag
     8101 aagtccgcga actggaagaa cataaccgcg aattgctcaa tccagccaca acgcgagaac
     8161 tgaccagtct ggtacgaaac ctgaaccgat tgttaaaaag tgaacgcgaa cgttacgaca
     8221 aataccgtac gacgctcacc gacctgaccc atagtctgaa aacgccactg gcggtgctgc
     8281 aaagtacgct gcgttctctg cgtagtgaaa agatgagcgt cagtgatgct gaaccggtaa
     8341 tgctggagca aatcagccgc atttcacagc aaattggcta ctacctgcat cgtgccagta
     8401 tgcgcggcgg gacattgctc agccgcgagc tgcatccggt cgccccactg ctggacaatc
     8461 tcacctcagc gctgaacaaa gtgtatcaac gcaaaggggt caatatctct ctcgatattt
     8521 cgccagagat cagctttgtc ggtgagcaga acgattttgt cgaggtgatg ggcaacgtgc
     8581 tggataatgc ctgtaaatat tgcctcgagt ttgtcgaaat ttctgcaagg caaaccgacg
     8641 agcatctcta tattgtggtc gaggatgatg gccccggtat tccattaagc aagcgagagg
     8701 tcattttcga ccgtggtcaa cgggttgata ctttacgccc tgggcaaggt gtagggctgg
     8761 cggtagcccg cgaaatcacc gagcaatatg agggtaaaat cgtcgccgga gagagcatgc
     8821 tgggcggtgc gcggatggag gtgatttttg gtcgccagca ttctgcgccg aaagatgaat
     8881 aaatatgtcc atacttcacg cattacgtta agcatccgtt ataatcggtt gcagatacca
     8941 gcctgtggat gcttaacatg gaataccaac tcactcttaa ctggcccgat tttcttgaac
     9001 gtcactggca gaaacgcccg gtggtgttaa aacgcggctt taataatttt attgacccga
     9061 tctctccaga cgagttggcg ggtctggcga tggaaagcga agttgacagt cgactggtca
     9121 gtcaccagga tggcaaatgg caggtcagcc acggcccgtt cgaaagctac gatcatctcg
     9181 gtgaaaccaa ctggtcatta ctggtacagg cagtgaacca ctggcatgag ccgaccgccg
     9241 cgctgatgcg accgttccgt gaactaccgg actggcgtat tgatgatctg atgatttctt
     9301 tttctgtacc tggcggcggc gtcggcccgc atctcgatca gtacgacgtg tttatcattc
     9361 agggtaccgg acgtcgtcgc tggcgagtgg gcgaaaggct gcaaatgaaa cagcactgcc
     9421 cacatccgga tctgttacag gtcgatccgt tcgaagccat catcgatgaa gagctggagc
     9481 ctggtgatat tctttatatt ccgccaggat tcccgcatga aggctacgcg ctggaaaatg
     9541 cgatgaacta ttccgtgggc tttcgcgcgc caaatacgcg ggaactgatt agtggatttg
     9601 ccgattatgt gctgcaacgt gaactgggcg gcaactacta cagcgatcct gatgttccac
     9661 ctcgcgctca tcctgcggat gttctgcctc aagagatgga taaactgcgt gagatgatgc
     9721 tcgaattgat caaccagccg gaacacttta agcaatggtt tggcgagttt atatcccagt
     9781 cacgtcatga actggatatc gcgccgccgg aaccgcctta tcagccagat gaaatctacg
     9841 atgcgctgaa acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta ttgcgcattg
     9901 gcgacgacgt gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg gcactggatg
     9961 cactcgccag caacattgcg ctgactgcgg agaattttgg cgatgcgctg gaagatccgt
    10021 cattcctcgc gatgctcgcg gcgctggtca atagcgggta ttggttcttc gaagggtaag
    10081 tttggattta agccggatgc ggcatcacat gccgcatccc ttttcgctta cttccgttgc
    10141 gccgttaact cggcaatacg gacgatcacc tgcaccgctt tttccatacc ttccagagtc
    10201 acaaactcat gcttaccatg atagttgtaa ccgccagtga acaagttcgg gcacggtaat
    10261 cccataaacg acaactgcgc gccgtcggta ccgccgcgga tcggtttcag ttccggttca
    10321 atatcgcagt cacgcatcgc ctgctgggcg atatcgagaa tatgcggatg ctcaaccact
    10381 ttctcgcgca tattgtagta actgtcttca atcaccagtt caatgtagca atcaggatgt
    10441 aaccctttgc ccactttttt ggcgatctcc atcattttac atttacgcgc ttcaaactgt
    10501 ttacggtcga aatcacggat gatgtagtgc atatcggccc gttcaacggt gcctttcatg
    10561 cccgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc atccgccgga
    10621 acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc tttcgccgtg
    10681 cccggatgaa cattgttacc gacaattttg atattgaccg atgcggcgtt gaagttttca
    10741 aactccagtt cgcctacgcc accaccgtca acagtgtaag cccagcgggc atcgaaggca
    10801 tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa ggcgacgcga
    10861 atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat gatttctgca
    10921 atacctgctt tgtcatcggc acctaacaag gttttaccat cggtggtaat caacgtctga
    10981 cccagtagct gatgcagcac cgggaacata accggtgata aaacttcatc gccgataccc
    11041 agcgcaatat cgccaccgcg atagttttca acaatttgcg gattaacatt tttgccgctg
    11101 caatccggtg aggtatccac atgagaaata aagccaatcg ccgggatatc gccagggacg
    11161 ttagccggta acgtcgccat caaagtgccc ttctcactta aggtcacatt gataagcccc
    11221 atttcttcga gctgctcttt cagcagatgc aataacttcc attggccttc cgtgctggga
    11281 acctgtctca cccctgcttt tgattgggta tccagagaca cgtagttcaa aaatcgctca
    11341 agtagtttat ccatgtagtc accctcactt tttgtgacaa cattattaag aagcaagaaa
    11401 agacaaatat tgcgtcaggt cacttttacc cctgcaagcg ggaatattta tcagcattac
    11461 cttaatgaat ataaagctaa gccagtaatt cacaacaaag attggcgatt caagcggttt
    11521 gccgtagaat tacagccctc attaagagtc accaaggtgg ttaaccacaa accccgcatc
    11581 ggtaagccat ccgttgcgtt tacatgggac agagtaaaaa attgaataaa caaccgagtt
    11641 cgctttcacc gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt aaagaggtca
    11701 ttccccagct ggatctgact atcaacaatg gcgagttcct cacgctgctt ggcccttctg
    11761 gctgcggtaa aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt gattccggac
    11821 gcatcatgct ggataacgag gacatcaccc acgttccggc ggaaaaccgc tatgtgaaca
    11881 ctgttttcca aagctacgca cttttccccc acatgaccgt gttcgaaaat gtggcctttg
    11941 ggttgcgcat gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg gaagccctgc
    12001 ggatggtgca actggaaacc ttcgctcaac gtaaaccgca tcagctctct ggtggtcaac
    12061 agcaacgcgt cgccattgct cgcgcggtgg ttaacaagcc tcgtctgttg ttgctggatg
    12121 agtcgctctc agcgctggat tacaaactgc gtaagcaaat gcagaacgag ctgaaagcgt
    12181 tacagcgtaa gcttggcatt acattcgtct tcgtgactca cgaccaggaa gaagcactca
    12241 ccatgtcaga caggattgtg gtgatgcgcg agggtcgcat tgagcaagac ggcacgccgc
    12301 gtgaaatcta cgaagagccg aaaaacctgt ttgttgccgg cttcattggc gaaattaata
    12361 tgtttaacgc cacggtgatc gagcgactcg acgagcagcg cgtacgcgcc aacgttgaag
    12421 gccgcgaatg taatatctac gttaacttcg ccgttgaacc ggggcaaaaa ctgcatgttc
    12481 tgctgcgccc ggaagactta cgtgttgaag agattaacga cgacaaccac gctgaagggc
    12541 tgattggtta cgttcgtgag cgtaactaca aaggcatgac gctggagtcg gttgttgaac
    12601 tggaaaatgg caagatggtg atggtcagcg aattcttcaa tgaagacgat cctgactttg
    12661 accactctct cgaccaaaaa atggccatta attgggtaga aagctgggag gtcgtactgg
    12721 ctgatgaaga acacaagtaa gttccagaat gtagtgattg tcactattgt cggttggctt
    12781 gtgttgtttg tctttctgcc caacctgatg atcattggca ccagcttttt gacccgcgac
    12841 gacgccagtt tcgtcaaaat ggtctttacg ctggataact acacgcgtct gctcgatccg
    12901 ctctattttg aagtgctatt gcactcgctg aatatggcgc tgatcgccac cctcgcctgc
    12961 ctggtgctgg gctatccatt tgcctggttt ctggcgaagc tgccacacaa ggtgcgtccg
    13021 ctgctgctgt ttctgctgat tgttccgttc tggaccaact cattgattcg tatctacggg
    13081 ctgaaaattt tcctcagcac caaaggctat ctcaacgagt ttttgctctg gctgggcgtt
    13141 atcgacacac caatccgtat catgttcacg cccagtgcgg tgattatcgg tctggtttac
    13201 attctgctgc cgtttatggt gatgccgctg tactccagta tcgaaaaact ggataagccg
    13261 ttgcttgagg cggcgcgcga tctcggtgcc agcaagttac agacttttat ccgcatcatt
    13321 attccactga cgatgccggg gattattgcc ggatgtctgc tggtgatgct gccagcgatg
    13381 ggcctgttct atgtgtccga cctgatgggc ggtgcgaaaa acctgctgat cggtaacgtc
    13441 atcaaggtac agttcctcaa tattcgtgac tggccgtttg gtgcagccac cagcattacg
    13501 ctgactatcg taatgggcct gatgttgctg gtttactggc gcgcttctcg tttgctgaat
    13561 aagaaggtga gcgaattaga tgattaatca tttatattca atgcattaag tttgtttttg
    13621 agtggttttg ccgtttaagg taacggcgtt atagatccta tcaaaatcaa tcaattccat
    13681 tctggttttg gagaaagatt tttcgagagc ttcggtcttt aactaaaact gattgcgtat
    13741 aaccccaata cccctccccg actatctgaa tggtcgggga ggcacttcac gcacaaaata
    13801 tcatccctgc gccgttgatt actctgcagg tgaagtcggc cactcaatat caggtgcagt
    13861 tgatgtatca acacggttca gcaacacctg aagtggcaca ctgaatttgg ccacctgaac
    13921 agaggtgata tgctcacctc agaacaacac aggtgctcca atg
//
LOCUS       DAJTNA010000101        13955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTNA010000101 DAJTNA010000000
VERSION     DAJTNA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13955
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.102"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            529..957
                     /locus_tag="OO161_002554"
     CDS             529..957
                     /locus_tag="OO161_002554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463585.1"
                     /translation="MAFDFYRINRFGRWEKYYNIQLRGALLSAINHLFTENNLDTEAI
                     TVSYEYILSRHLIANTEFSYLAFPDNYNRLFIPQPKTKAGNGSKHLIVKRLADCLLLA
                     AYTMGISKDSGKQQTSWGEMHLLVTINLWITKGYSSLVLQ"
     gene            882..1424
                     /locus_tag="OO161_002555"
     CDS             882..1424
                     /locus_tag="OO161_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HCR5463586.1"
                     /translation="MGGDAPAGYDQFMDNKGLLIAGASIVAGAVLGRMRLPDLESLEH
                     FGARGAVSGRPFNPDLAGGPIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMIYR
                     LKDIEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPDGDAGYALWNHTHTATLED
                     YKLKGELNDLYHQDALDYDN"
     gene            1414..1659
                     /locus_tag="OO161_002556"
     CDS             1414..1659
                     /locus_tag="OO161_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5463587.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(1944..4205)
                     /gene="metE"
                     /locus_tag="OO161_002557"
     CDS             complement(1944..4205)
                     /gene="metE"
                     /locus_tag="OO161_002557"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5463588.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREKLLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4441..5394
                     /gene="metR"
                     /locus_tag="OO161_002558"
     CDS             4441..5394
                     /gene="metR"
                     /locus_tag="OO161_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5463589.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5282..6181)
                     /gene="bioP"
                     /locus_tag="OO161_002559"
     CDS             complement(5282..6181)
                     /gene="bioP"
                     /locus_tag="OO161_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5463590.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAYLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(6257..7057)
                     /gene="yigL"
                     /locus_tag="OO161_002560"
     CDS             complement(6257..7057)
                     /gene="yigL"
                     /locus_tag="OO161_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5463591.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            complement(7065..8087)
                     /gene="pldB"
                     /locus_tag="OO161_002561"
     CDS             complement(7065..8087)
                     /gene="pldB"
                     /locus_tag="OO161_002561"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5463592.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEVHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8198..8818
                     /gene="rhtB"
                     /locus_tag="OO161_002562"
                     /pseudo
     CDS             8198..8818
                     /gene="rhtB"
                     /locus_tag="OO161_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
     gene            complement(8880..9500)
                     /gene="rhtC"
                     /locus_tag="OO161_002563"
     CDS             complement(8880..9500)
                     /gene="rhtC"
                     /locus_tag="OO161_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5463593.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9564..11393)
                     /gene="recQ"
                     /locus_tag="OO161_002564"
     CDS             complement(9564..11393)
                     /gene="recQ"
                     /locus_tag="OO161_002564"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5463594.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11526..12395)
                     /gene="pldA"
                     /locus_tag="OO161_002565"
     CDS             complement(11526..12395)
                     /gene="pldA"
                     /locus_tag="OO161_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5463595.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12560..13027
                     /locus_tag="OO161_002566"
     CDS             12560..13027
                     /locus_tag="OO161_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5463596.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTTTSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            13079..13955
                     /gene="rarD"
                     /locus_tag="OO161_002567"
                     /pseudo
     CDS             13079..13955
                     /gene="rarD"
                     /locus_tag="OO161_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
BASE COUNT         3494 a         3640 c         3464 g         3357 t
ORIGIN      
        1 attacgactc cgctatttga ccttaagaaa gtcggcaagt catagtcata aatgagtctc
       61 ttcaatggaa gacaccttag agatttacca tctactccca ttcacaaaca aacattaatt
      121 attatcaaca cattcacatt aattataaat catctcagac cttcctgata aaaagatatt
      181 aaagtgaaac aatttgtttt tcacatttta gttcatcact tttaggcgta aataaatgta
      241 aaaaaaacaa caatactatt tctctttcac aaaagaatta acaaaaggtt ttttaaggat
      301 gagtgatatt atttacttga ccataaccgg agaacaacaa ggatgtattt cctctctttg
      361 tggaacgagc gcatcaatag gtaatcgctg gcagattggc catgaagatg aaatcttcgc
      421 gttctcgctc tctaatagta ttacgaatgc aggcaaaggg tcacaattgc atggccttag
      481 tttctgtaaa ttcttattaa cgctattaac aataacgagc agttgttcat ggcgtttgac
      541 ttctatcgga tcaatagatt tggtcgatgg gagaaatatt acaacattca actccgcggc
      601 gcgctcttat cagcaatcaa tcacctgttt actgagaaca atctggatac agaagccatc
      661 actgtcagtt atgaatatat actctccagg catctgattg ctaatactga atttagttat
      721 ttggcatttc ccgataacta caaccgtctg tttataccac aaccaaaaac taaagctggt
      781 aatggttcaa aacacttaat agtaaagcgg ttggcagact gcttgctgct ggcggcatat
      841 acaatgggaa tatcgaagga ttcagggaaa cagcagacaa gttgggggga gatgcacctg
      901 ctggttacga tcaatttatg gataacaaag ggttactcat cgctggtgct tcaatagttg
      961 ctggagcagt tctggggaga atgagactcc ctgatttgga atcacttgag catttcggag
     1021 ccagaggagc agtatcagga agaccattta atcccgacct tgccggtggt ccaatagaaa
     1081 atctgacgac agatggcgta acaatcaaca gggaaggtat cgctattgtt gaaaaacaca
     1141 tagccagatt tggtcatgac cctgtaaatg aagtgatgat ctatagactg aaagacattg
     1201 agaaaggaaa aatcccccca gaacaagtag atcttaactt ctatactcat gagtgtcggg
     1261 aatatcagcg ttactgtaat cttggttggg aaacaggaca acctgatggt gacgcaggct
     1321 atgcactctg gaatcatacg catactgcta ctctagaaga ttataaactt aagggagaac
     1381 ttaatgactt ataccaccag gatgcacttg actatgataa ctgaaactga aattaacgta
     1441 ctgaaagtta tggctgagaa agatataaac tggaactgga tgaatctcga tcgcacacta
     1501 tccataaggc aaattccagg attcggtaat gttgttaaca ttgttaacaa attagctaac
     1561 gagggtttgg ttattattga ggatagcgga cataagtcta tgcctcacta ccacgttact
     1621 gaaaagggat acaatttcgt gaaaagtgag aataaataac gtagcgttga ttactgaaca
     1681 aatcgctcag gattatattt tgcgtaaaaa tgattcggtt atttctgtat ctgaacgggc
     1741 gcagatagca aaatggcttc cggtgatgga agccatcaga ttattggcaa agtgcgcgtt
     1801 gttcatgccg gatgcggcgt gaacgccttg tccggccaac aaaactcaaa aattcaataa
     1861 attgcataaa ttatgtaggc ctggtatcag gcagttttgc ctctttcatc gtactgcaat
     1921 ttacagtgac tcgttaatat tcatcatccc cgacgcaaat tctgcgccgc ctgcaccatg
     1981 ttcgccagtg ccgcgcgggt ttctggccag ccgcgcgttt tcaggccaca gtccgggttg
     2041 acccacaggc gctctgccgg aatgcgtttt gccgctttct tcagcaaggc ttcaatccat
     2101 tctacgctcg gtacgtttgg cgagtgaatg tcatagacgc ctggaccgat ttcattcgga
     2161 taatcaaact cttcaaacga ctccagcaac tccatgtcgg aacgcgaggt ttcgatggtg
     2221 atgacgtctg catccagtgc cgcaatggaa tccatgatgt cgttgaactc gcaataacac
     2281 atgtgagtgt ggatttgtgt gtcatctttc gccacggcgg cgttgatacg gaaggcctcc
     2341 acgccccact ggagatatgc atcccagtcg ctacgacgca gcggtaaacc ttcgcgcagc
     2401 gccggttcat caatctggat gatgccaatt cctgcggctt ccaggtccgc cacttcatca
     2461 cgcagcgcca gcgcaatttg tttggcgatg gtttcacggc tgacatcttc acgcgggaac
     2521 gaccagcaga gaatggtcac cgggccagtc agcatccctt tcaccggttt atcagtcaga
     2581 gattgcgcat acttcgccca ctccacggta atcggtgccg ggcgactaac gtcaccaata
     2641 acaatcggtg gcttcacgca gcgggaaccg tagctctgta cccaaccgtt ttgcgtaaag
     2701 acaaatccgt cgaggtgctc gccaaagtat tccaccatgt cattacgctc ggcctcgcca
     2761 tgtaccagca catccagtcc caaacgttcc tgctcaacaa tggcctgctt gatatgttcc
     2821 gcaatgcccg tgcggtagtt gttggcgtcg agattgccct ttttgaaatc cagacgcagg
     2881 gtacgaattt ccgtggtttg cgggaaggaa ccaatcgtgg tggtcggcca cgctggcagt
     2941 ttaaaacgcg cacgctgggc ttcagcacgc acttcataga cattcgcacg ctggctgtcc
     3001 tgggcggtga tcgccgccag acgcttttct accgccggat tatgtacacg agtagagtga
     3061 cgacgcgcct gaattggggc gctccactct gccagagctg ccgtgtcacc actgttcagc
     3121 gcatcgcgca gcagtgccag ttcatggcat ttttgtaagg cgaaggcaaa ccagcttttc
     3181 acttctgcat caagacgcgt ttccacgctc aggtcgatgg ggctgtgcag caacgagcag
     3241 gaagatgcca cccacaaatc acgtttgccg acaatgtcct taatttgcgc atatttctca
     3301 gtaagatcgg cgcgccagac gttacgacca ttgatcagtc ccgcagacag cagccagtca
     3361 gaaggcaggc gcttgtgcag ttcagcaacg ccatctttac catgtacaag gtccacatgc
     3421 agtccctgaa caggcagcgc agtaatcgtg tcgagatttg gcgtaacgcc ttcaaaatag
     3481 gtagtcagca gcagtttcac ctgtccctgg agcgcgtcgt aagcgggttt gtatgcgtcc
     3541 agccacgcct gtggtagttc cagtaccagc gcgggttcat caatctgtac ccactcgatg
     3601 ccgcgtttct ccagttctgc cagcacttgc tgataaaccg gcagaatgtc gttcagcagg
     3661 ctcaggcggt caaactgttc acctttcact ttccccagcc acagccaggt aaccggcccc
     3721 agcagcacag gcttaacgtt gtggcccagc gccagcgcct cgtccacttc ctccagcagc
     3781 tgcgtccagg tcagtttgaa ctgttggcct ttaacgaatt ccggcaccat gtagtgatag
     3841 ttggtgttaa accatttggt catttccgct gccgccgcag gttcgccagt cggcgcacgt
     3901 ccacgaccaa tacggaacag ggtgtcaata tctaccgaac catctttgtt ctgatgacgc
     3961 gccggaacgt tacccagcag cagactggtg gtcagtacat gatcgtacca ggcaaaatcg
     4021 cccaccggca gcaggtcgat acccgcttgc ttttgttgat cccagtgacg agcacgcaat
     4081 tcacgcccta ccgccagcag tttttcacgc gtggagttcc ctgcccaata actctcttgc
     4141 gcttttttca gctcgcgacg caggccaacg cgagggaaac cgagggtgtg attcaatatt
     4201 gtcatttttc ttcctctaat tatatgtaaa tcctatggat tttgaattta gggaaggcgg
     4261 caagtttatt catccccggg agcttactga agtaagtgac cggggtggag aaacgcagcc
     4321 aacgcaccat aaatttaaaa gacgaaggat tttagccgtc cagatgttta cacatccata
     4381 attggcggtt actgtatatt cctcaagcgc aaattattca tgccgaagtg aaggactttc
     4441 atgatcgaag taaaacatct gaaaacgcta caagcgttgc ggaactgcgg ctcactcgca
     4501 gccgctgcgg cgacgttgca tcagacgcaa tccgccctgt ctcaccagtt tagcgatctg
     4561 gaacaacgcc ttggcttccg gctatttgtg cgtaaaagcc agccgcttcg ctttacaccg
     4621 cagggagaaa tcctgttgca actggcaaac caggtgctgc cacaaattag ccaggcactg
     4681 caagcctgca atgaaccgca gcagacgcgt ctgcgcattg ccattgagtg ccatagctgt
     4741 atacagtggc tgacgcccgc gttagaaaat ttccataaga actggccgca ggtcgagatg
     4801 gactttaaat cgggcgtgac atttgacccg cagcccgcct tgcaacaggg agagctggat
     4861 ctggtaatga cgtccgatat tctgccgcgc agtggtctgc attattcgcc gatgttcgac
     4921 tatgaagtgc gtctggtgtt agcacctgac catccactgg cggcaaaaac gcgtattaca
     4981 ccggaagatc tcgccagcga gacgctatta atttatccgg tgcagcgtag tcgactggat
     5041 gtctggcggc attttcttca gccggcaggc gtcagtccgt cactgaaaag cgtcgataac
     5101 accttattgt tgattcagat ggttgccgcg cggatgggta ttgccgcgct accgcattgg
     5161 gtagtagaga gttttgagcg ccagggtctg gtggtgacca aaaccctggg cgaaggcttg
     5221 tggagccgac tgtacgccgc cgtgcgcgat ggcgagcagc gtcagccagt tacggaagcg
     5281 tttattcgct cagcgcgcaa tcacgcctgc gatcatctgc cgtttgtgaa gagcgcggag
     5341 cgacccactt acgatgcacc cacagtgagg ccaggatcac cagcgcgcct gtaataaacg
     5401 ttggccagtg cggctgttgg tgccagatag ccaggtttac cagcagccct gccggaacgt
     5461 gcatattatt cataattccc agcgttccgg cgtccacctg cgtcgcgccg tagttccaca
     5521 taaagtagcc aatcccggaa gccaccacgc caagaaacac cagaatgccc cattgcaacg
     5581 tggtttgcgg cattttctgc gcatttccca gcaagaacca tgcaattacc gccaccaggt
     5641 acgcgccaag atagaaccac gcaaaggcgt tatgctgcgg catcgggcga gtttccatca
     5701 ggcgtttgta acccaccatg ccgatggcaa aagtgatatt ggagagttgc accagcaaca
     5761 agccagtcca gaaatggtcg gtgacctgat catagcgaat aatcccggca ccaatcaccg
     5821 ccaacaaggc actaaaagca tatccccagc gcagacggcg cttattcatg atgtcataaa
     5881 tcagcgtgat gtagagcggc gtcagtacgg tgaacagcag cagttcggaa accgtcagat
     5941 agagataagc gcggaaactc agcatataca tcacgccgag ctgcatcgcg cccaccagca
     6001 tatacaggcc gactgttttc aggctattgc cacgggtacg cagaaacggc agaaaaacga
     6061 gtgccgccag gccaacgcgc accagcaccg caaaatagct atcgacgtgc cccgcaaggt
     6121 actcgccata aaagctaaag gagaaggccc acagaatcgt ggtgatgata agtagcgcca
     6181 caatggaaaa ctctttgatt aacgagtatc cattgtagcg aagtatcagg ttgacaactg
     6241 accaaataaa gaacgattac gataaataga gtttacgcag ataatgcggc accgcgtcgt
     6301 cggcattagt accaatcact tccagctcgg gatgaaggtc tttcagacgc tggtgcgcac
     6361 tgcccataat gcagcctttc cccgccatcg acagcatttc ggcgtcgttc atcccgtcac
     6421 caaacgcaat acaatccttc aggctgtagc ccagtttctt cgccaccgct tccagcgcat
     6481 ggccttttga aacgccgccc gccatcactt ccagacaggt taaggtagag aaactgacgt
     6541 tgacgcgatc gccccaacga gcgttaatcg cctgctccag cggcagcagt tgttcatggg
     6601 aatcgcaggt gaagaacact ttgctgacgc cttccggctc cagtaatcca ggctcataca
     6661 gcgcatattt gaacaccgct tctttaaaaa agcgcatctc ttccgggcga tggcgattca
     6721 taaaccattc gtcgtcgcga taaacgttag taatgatgtc cggattgtcg ttgactacgc
     6781 caaacagatc gctggcaatg tcgcgatcca ggttatgagc aaaaatcaga ttaccatcca
     6841 gatcgtgaac gcgcgcacca ttggaggtaa tcatgtaaga cttaatctcc agattatcgc
     6901 gaatttgccc cacatcaacg tggtgacgac cggtcgcaaa cacaaagttg atgccgcgcg
     6961 cggtgagcag cttcagagtt tctttggcgt aaggggataa cgtatggtcg ggagaaagta
     7021 acgtgccatc taaatcagac gcaacaacct ggtacataag aaatttaaac ctctgtagag
     7081 cggtttccgc tgggtgagtt atgcctgttg aaaaaatcaa cgatggcgtg gagcgcgact
     7141 gagcgcattg cgtccttttc aaaaaggatc tcatggtaag cacctttaat taccaacggc
     7201 cgtcctcctt cgacaggatg gcccgcggcg gtgcggagtt cacaaaaacg gtcatgcatg
     7261 cggttatcca ccacgcgttc ctcttcagcc tgcaagagaa gcgttggcgt ggcgtcatca
     7321 cccgcaccgg ctaacacctg ttcgccagcc agaatacttt cgcgtaccca atggtaggtc
     7381 ggcccaccga cgcgaatcgt tgggtcatca gcatagaagc gtaagttacg tcgatatcgc
     7441 tgtctgctgt gggtcagtac gttgatagca aacggcaacg cgcgccagcg cccggtgcct
     7501 attgcatagc catcacggaa acgtggatgc acttcggccc agttgaggat ctgccgtgcc
     7561 ataaatgacg gcatacgaat cacgatccca aacattggcg cagttagcgc aatggcgtca
     7621 catacacctg gatggcgttg cagaaataat gtggagatcg caccgcccat cgaatgtgcc
     7681 agtatatagc gtttacgcca cggaccgggc tggacctcct gctgccagaa tgccgccaga
     7741 tcatcaacat aatcattaaa gcgattaaca tgcccgagat gcggatcggc taacaggcga
     7801 ccggaacgtc cctgcccgcg atggtcgatg attaagacat caaaccccaa atggaacagg
     7861 tcataggcca gttccgcata ttttacgtag ctctcaatac gccccgggca gatgactacc
     7921 acccggtcat ggtgctgtgc gcgaaaacgg acaaagcgca ccggaatgtc atccacacca
     7981 gtaaactctg cttcatcacg ctgacgccag aaatcagtca gcggtcccat ggtaaaagca
     8041 gcaaacgcgt tttctcttgt ttcccagtct ttttgctgct gaaacatcgg gtaatctgcc
     8101 tcttaaacca cgtaaaatcg ttttttttag cgtgcctgac acaacgctgc gacagtagcg
     8161 tattgtggca caaaaataga cacaccggga gttcatcatg accttagaat ggtggtttgc
     8221 ctacctgctg acatcgatca ttttaagcct gtcgccaggc tctggtgcaa tcaacactat
     8281 gaccacctcg ctcaaccacg gttatcgcgg cgcggtggcg tctattgctg ggcttcagac
     8341 cggactggcg attcatattg tgctggttgg cgtggggttg gggacgctat tttcccgctc
     8401 agtgattgcg tttgaagtgt tgaagtgggc aggcgcggct tacttgattt ggctgggaat
     8461 ccagcagtag cgcgccgctg gtgcaattga ccttaaatcg ctggcctcta ctcaatcgcg
     8521 tcgacatttg ttccagcgcg cagtttttgt gaatctcacc aatcccaaaa gtattgtgtt
     8581 tctggcggcg ctatttccgc aattcatcat gccacagcag ccgcaactga tgcagtatat
     8641 cgtgctcggc gtcaccacta ttgtagtcga tattattgtg atgatcggtt acgccaccct
     8701 tgctcaacgg attgctctat ggattaaagg accaaagcag atgaaggcgc tgaataagat
     8761 tttcggctcg ttgtttatgc tggtgggagc gctgttagca tcggcgaggc atgcgtgaaa
     8821 aataatgtcg gatgcggcgt aaacgcctta tccgacttac tctgaagacg cgtctggcat
     8881 caccgcgaaa taatcaaatg aatgccaaat ccggcaaata acgccccggc aaaaccatca
     8941 atccacttcg ccagacgttg ataaccacgg cgcatttgcg gcagggcaaa caggctggca
     9001 acgacggtaa accacgccag tgtttcgaca atgatcagcg caaaaatgcc ccagcgcgcg
     9061 gtagtgccaa cgttatcacc gacaaacaat gagaacaccg agccaaagta gataatcgct
     9121 ttcggattag cgagattggt cagtaaacct ttcaggaaac tgcgcccgct tttcgccagc
     9181 tcaatctgtg gtgcaggcgc agatacagcc tcttttttca gtgcaccgcg tagcatctgg
     9241 taacccatcc agcagagata caggccaccg ccaaccataa tcagcgtatg cagccaggcc
     9301 attttttcga taatcaaatg caggccaagc aacgcaatcc ctgcccaaac cataacgccg
     9361 caggtaatac ccagcacgcc catcatcgct tctttacggg aacgactgac agcggtctga
     9421 gagacaaaaa agaaatcggg accggggctc ataagcgcca caatgtgcac catggcgacg
     9481 gtgagaaata acatcaacat acatttgact cgcgggggaa acgtttacgg agtcttcatc
     9541 ctggcacttt tttatgctgc tgactactct tcgtcgtcgc catcgacatg cgcacgaatc
     9601 agcgccataa acggtttacc aaagcgttcc agcttgcgca tcccaacgcc gttaacgctg
     9661 agcatttcgc tggcggtgat cggcatctgt tcagccatct caatcaaggt tgcgtcgtta
     9721 aacaccacgt acggcgggac attactttca tcggcaatcg atttacgcag tttgcgtaat
     9781 ttggcgaaca gtttgcgatc atagttgccg ccgaacgatt tctgcatcgc tttcggtttg
     9841 agcgccacga tacgcggcac ggcaagttgc aaagaggatt cgccgcgcag caccgggcgc
     9901 gcggcctctg tcagttgtag ggcagaatgc tgggcaatat tttgcgtcac caggccgagg
     9961 tgaatcagct ggcggatcac gctcacccaa tgttcatggc ttttatcacg gcccatgcca
    10021 tagactttca gtttgtcatg accatagtcg cggatacgct ggttattagc accacgaatc
    10081 acttccacca cataacccat cccaaaccgc tgattcacac gaccaatggt ggaaagggca
    10141 atctgagcat cggttgaacc gtcgtactgt ttcggcggat cgaggcagat atcgcagttc
    10201 ccgcacggct cttgccgccc ttcgccaaaa tagttcagca ataccagacg acggcaagtt
    10261 tgcgcttcgg caaaagcgcc catcgcattg agtttatgac gttcgatatc ctgtaactgc
    10321 ccctgcggct tctcttccag gcaacggcgc agccacgcca tatcggccgg atcgtaaaac
    10381 agcatcgctt ccgcgggcag gccatcacgc ccggcgcgac cggtttcctg ataataggat
    10441 tcgatattgc gcggaatatc aaagtggacc acgaagcgaa cgtttggttt attgatgccc
    10501 atgccgaacg ccaccgtcgc caccacaatt tgcaggtcat cgcgctgaaa tttttcctgc
    10561 acgtcggcgc gaacattatt ttccagccca gcatgatagg ccgccgcgct aattcccttg
    10621 ctttgcaggc gcgcagcggt gtcttctact ttcgcgcggc tgttgcagta gataatgcct
    10681 gacttaccgc gctgttcctg cacgtagcgc atcaactgat cgagcggttt gaacttctcc
    10741 atcagcatgt agcgaatatt cggacggtca aaactgctga tttgaatcag cggatcgttc
    10801 agccccagca agcgcacgat atcctggcgc gtggtgtcgt cggctgtggc agtcagcgcc
    10861 ataaacggca gggtcgggaa acgctggcgt aactgaccaa gcgcggcata ttccgggcgg
    10921 aaatcgtggc cccattggga gatacagtgc gcttcatcaa cggctaataa caccggattc
    10981 cagtgcgcca gatgctcaag aaagttatcc agcatcaggc gttccggggc gatataaagc
    11041 agacgaattt gcccggtgcg gcagcctgtc atcacttcaa gttgctgctc gcgggtttgc
    11101 gtcgagttaa ggcacgccgc cgccacgccg ttggcttgca gttgatccac ctgatctttc
    11161 atcaacgaaa tcagcggtga aacaaccacg gtaaggccgt ttagcaataa ggcaggaatt
    11221 tgatagcaaa gggattttcc gccaccggtg ggcatgacga ccaggcaatc gcgaccggaa
    11281 agcacagtgt cgataatttc ttcctggccg gggcgaaact gttggtagcc aaaggtttct
    11341 tgtaaaacct gtttagctcc ggactccaga ttcaacactt ccgcctgcgc cacattcacc
    11401 ccgattgcct gaaataaaaa caggcgctat tttcagcgcc tgcgcgagaa actgcaatgc
    11461 ttttagtctc tgagagggta atcctggatg ctgggttcca gatttgccaa ccactcaacc
    11521 gttcatcaaa acaagtcgtt tagcataact cctacaccga cacgggtctg gttgaagtta
    11581 tagtcgatga gcgattcgcc atagccgctg taaacctgag tataaaggcg cacatgtttg
    11641 gtgatcgggt aacttaagcc taactccgcg ccgccgtaac cggtattcca gttgtactgt
    11701 cctttcgcac tcaacaccgc atcaccgagg tgatagccga ttttaagctg gtagtaaccc
    11761 atatatttgg tgatatccgg gttatcgtca gtattaccca ccacatacca cggcttcact
    11821 tctaccagcc agttaccgtt ttctgccatc aggcgagtat aaaggcggtt ccagctgcgg
    11881 gaggtcgggt cggaacgccc gttagagtcg tggttatacc ccatctccac atcgcgcagc
    11941 gtccaacctg caaaacggta atcggtggca aaaccgagga acaattgcgg ttcgtagttg
    12001 gtttcacgaa acggtgaaga ctcttcgcta ttggacagtt gccaccagga tttttgcgta
    12061 taagacgcac ccaacaccga gttcgggcct aaaatcccac gccacagtgg aaatgccagg
    12121 ctcaactgaa actttacttc atccttacgc gcattttccg cccagtcgta actggcaatc
    12181 gcttctttgt tcagatcgct ggtttgggtg tagatgaggt agttggtgtc ataaggatag
    12241 agcgtgaacg gattgtcatg ctcctgcagc atattggcga taatactgcc acgcaccgct
    12301 ggcgcgtcat gcacctcttt caccgttgcc tcttgtgcat atactgccat aggcaacata
    12361 aacaccggca acaaccagcc ctgcagagtc cgcatcggtc gtgttctccg taacgaatta
    12421 ttgataaggt gaatgttttt ctgcgggcta ttttacatac ttaactccat tgccgttagt
    12481 tatcccttcc taaagtagaa agcaacaaca aagaagcata aaatcaacat ttcattaacc
    12541 aatgaagtgt aaggatgata tgtctgccgt actgaccgct gaacaagccc tgaaattagt
    12601 gggtgagatg tttgtttatc acatgccatt taaccgcgcg ttagggatgg agctggagcg
    12661 ttacgaaaaa gagttcgcac agctggcctt taaaaatcag ccgatgatgg tgggtaactg
    12721 ggcgcaaagc attttgcacg gcggagtcat tgcgtcggcg ctggatgtcg ccgccggtct
    12781 ggtgtgcgtg ggaagtacct taacccgcca cgaaaccatc agtgaagatg aactacgcca
    12841 gcggctatcg cggatgggga ccattgatct tcgcgttgat tatctgcgcc caggcagggg
    12901 cgagcgtttt actactacca gtagcctgtt gcgtgcaggc aataaagtcg ccgtcgcccg
    12961 cgttgaatta cacaatgaag aacagcttta tattgccagt gccaccgcca cctatatggt
    13021 aggttgaaga cataaaagca ggtaaactgc tgttaccaat taaaaatgag tattcccgat
    13081 ggatgcaaaa caaacgcggc agggcgtatt actcgctctt gccgcttatt ttatttgggg
    13141 tattgcgcca gcatacttca agctgattta ctacgtgccc gccgatgaga tcctgacgca
    13201 tcgcgtgatc tggtcgtttt tctttatggt ggtgctgatg agcatttgcc gccagtggtc
    13261 ctatttaaaa acgctgattc agacgccaca gaaaattttt atgctggcag tctctgccgt
    13321 gctgattggt ggcaactggc tactgtttat ctgggcggtg aacaatcacc atatgctgga
    13381 agcgagcctt ggttacttta ttaacccgct ggtgaacatt gtgctgggga tgattttcct
    13441 cggcgagcga ttccgccgga tgcaatggct ggcggtgatt ctggcgatat gtggcgtgtt
    13501 ggttcagttg tggacttttg gttcgctgcc aatcatcgcg ctggggctgg cgtttagttt
    13561 tgccttctac ggactggtac gcaagaaaat tgccgttgaa gcgcaaaccg gcatgttaat
    13621 cgaaaccatg tggctgctgc ccgtggcggc gatttatctg tttgctattg ccgacagctc
    13681 aaccagccat atggggcaaa acccgatgtt gctgaatttg ctgctgatcg ccgccggtat
    13741 tgtcaccacc gtaccgctgt tgtgttttac cgcagctgct acacgcctgc gtctctccac
    13801 gttaggcttc ttccagtata ttggcccgac gctgatgttc ctgttggcag tgacgtttta
    13861 tggtgaaaaa ccgggtgccg attagatggt gaccttcgcc tttatttggg tggcgctggc
    13921 aatttttgtg atggatgcga tttatacgca gcgta
//
LOCUS       DAJTNA010000102        13795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTNA010000102 DAJTNA010000000
VERSION     DAJTNA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13795
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.103"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            65..172
                     /locus_tag="OO161_002568"
     CDS             65..172
                     /locus_tag="OO161_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5463597.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(321..1175)
                     /gene="kdsA"
                     /locus_tag="OO161_002569"
     CDS             complement(321..1175)
                     /gene="kdsA"
                     /locus_tag="OO161_002569"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5463598.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(1211..2020)
                     /locus_tag="OO161_002570"
     CDS             complement(1211..2020)
                     /locus_tag="OO161_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5463599.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMILAQINNIAHKHIVLH"
     gene            complement(2024..2416)
                     /locus_tag="OO161_002571"
     CDS             complement(2024..2416)
                     /locus_tag="OO161_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5463600.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(2413..3246)
                     /gene="prmC"
                     /locus_tag="OO161_002572"
     CDS             complement(2413..3246)
                     /gene="prmC"
                     /locus_tag="OO161_002572"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5463601.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(3246..4328)
                     /gene="prfA"
                     /locus_tag="OO161_002573"
     CDS             complement(3246..4328)
                     /gene="prfA"
                     /locus_tag="OO161_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5463602.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(4370..5626)
                     /gene="hemA"
                     /locus_tag="OO161_002574"
     CDS             complement(4370..5626)
                     /gene="hemA"
                     /locus_tag="OO161_002574"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5463603.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            5840..6463
                     /gene="lolB"
                     /locus_tag="OO161_002575"
     CDS             5840..6463
                     /gene="lolB"
                     /locus_tag="OO161_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5463604.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            6463..7314
                     /gene="ispE"
                     /locus_tag="OO161_002576"
     CDS             6463..7314
                     /gene="ispE"
                     /locus_tag="OO161_002576"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5463605.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            7465..8412
                     /gene="prs"
                     /locus_tag="OO161_002577"
     CDS             7465..8412
                     /gene="prs"
                     /locus_tag="OO161_002577"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5463606.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPMVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            8537..10216
                     /gene="dauA"
                     /locus_tag="OO161_002578"
     CDS             8537..10216
                     /gene="dauA"
                     /locus_tag="OO161_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5463607.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(10271..10549)
                     /gene="ychH"
                     /locus_tag="OO161_002579"
     CDS             complement(10271..10549)
                     /gene="ychH"
                     /locus_tag="OO161_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5463608.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            10827..11411
                     /gene="pth"
                     /locus_tag="OO161_002580"
     CDS             10827..11411
                     /gene="pth"
                     /locus_tag="OO161_002580"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5463609.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            11528..12619
                     /gene="ychF"
                     /locus_tag="OO161_002581"
     CDS             11528..12619
                     /gene="ychF"
                     /locus_tag="OO161_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5463610.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            13388..>13795
                     /locus_tag="OO161_002582"
     CDS             13388..>13795
                     /locus_tag="OO161_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463611.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIG"
BASE COUNT         3382 a         3520 c         3423 g         3470 t
ORIGIN      
        1 aatcttgaac catcaacaat tcaagaatag ccgcaaaatg ttgtcattac aacaaggcgg
       61 ctatatgacg ctcgcgaagt ttgccatgat tttctggcac gacctggcag caccgatcct
      121 ggcggggatt attaccgcag cgattgtcgg ctggtggcgt aaccggaagt aaggtgaaaa
      181 cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac cggccgcagg ggtaacagcc
      241 tgcggccttt tttgtatctg gcacccatga aaaaagtctt aacgcagaac gctaagactt
      301 tatttttcaa gcaaaaaaaa ttacttgctg gtatccagtt cttcgaaacc tttcaccaga
      361 tcatcaatcg ctttcatctg cttgaggaac ggttccagtt tagccagcgg cagcgcggat
      421 ggaccatcac atttcgcatg ttccggatcc ggatgcgctt caataaacag ccccgccaga
      481 cctaccgcca taccggctcg tgccagctca gccacctgag cacgacgacc accggaagct
      541 gcgccaaacg gatcgcggca ttgcagtgcg tgggtcacgt cgaaaatcac cggcgagtta
      601 ccagacactt tcttcataat gctgaagccc agcatatcga caaccaggtt gtcatagccg
      661 aagttagcac cacgatcgca aagaatcact ttttcgttgc cgccttcttt gaatttatca
      721 acgatattac ccatctgtcc cgggctgaca aactgtggtt tcttgacgtt aattaccgca
      781 ccggttttcg ccatggcttc aaccaggtca gtctggcgag caagaaacgc cggcaactga
      841 atcacatcca cgacatcagc aacgggctgt gcctgacttg gttcgtgaac gtcggtgata
      901 attttcacgc caaaagtctg cttcaactcc tggaagattt tcatcccttc ttccaggccc
      961 ggtccacgat aagagtggat ggaggagcgg ttggctttgt caaaagaggc tttgaacacg
     1021 taagggatac ccagtttctg ggtcacagtt acgtagtgct cgcaaatgcg catcgccaga
     1081 tcgcgagatt ccagcacgtt cataccgcca aacagtacga acggcaggtc atttgctacg
     1141 ttgatgtcgc caatgctaac cactttttgt ttcataggat cgccttaatc ttgagtaaaa
     1201 tgtcgattaa ttaatgcagc acaatatgtt tatgcgcgat gttatttatt tgcgcaagga
     1261 tcatttcgct gatcgggtct tccggacact gttcaacgaa ataacttaaa tcgttcaacg
     1321 caacgtgttc gcaatccagt tgcgcataaa tcaacccgcg atcgcgaatt tcatagggat
     1381 cttcagggtt gaattgtaat aaagcttcgc tggtgcgtaa cgccagctcc atctgatttt
     1441 cttccatcaa cgaggctttg agtgtatcca gcaatttgcg gattacctca atgttatcag
     1501 cttcatcaag gtcttcataa aacagttccg ccgacgggct gatatttccc tttaaccata
     1561 cgtccagcat atgttcgctt aacgattcac cgttaaaagg attaatcagc caaatttcgc
     1621 catccggaca ttcaatgcgc aatatcagct gcgtagggaa aatcaccggc agcagcggca
     1681 aatcgagacg attcgcgacc cataataaaa ccgcacctaa tgataccgca ctgccctgtc
     1741 gattctttaa cacctggtcc agccataatg catcggaaag acgataaacg ccgcgtgagg
     1801 ctttaaatcc ccagtcgccg taaaacagcg cgatcagttt ttccagctgc tcttctaaag
     1861 gcagaagctg gctgatttct tccttcgcca gactaacgag acgctccagt tcgtcgtaaa
     1921 catcttgcga gggaaaatcg cggcggattg cttcgcaagc caggatcatg ccttcgcaca
     1981 atggcgcttt attaaattcg aaatcagcta acgatctcat gacttacccc agtaacggta
     2041 cttttgtggt ggcgagttta atgatgatgt acagcaccac caacgccagc gggaaggcaa
     2101 taatgcgcgc ctgctgactg tgcatacgac gataatcgag tgcaataaaa cccaaaacga
     2161 tataaatgat aactccaaac agcttttcag tcagccactg tgcctgcccg gaaaatggca
     2221 ggatgtgcgc tttagccatc aacgcaatgc cgcttaacaa taacaccgta tcgacaaccg
     2281 gcggcacaat gcgcgtccag cgagcaaatg cctgtggatg cttctgataa cgtagccaga
     2341 aacgtaaggt taatagccca acagaaagcg cgatactaat aagatgaaca ctaagcagtg
     2401 tagaaaaact tgtcattgat aatagcggcc gagcgttacg cgctcgttat caccatagtc
     2461 acggcaggtt tcgacgtcat gataccccgc gaggataaat gcttgtcgca ccgcttcgcc
     2521 ctgctgccag ccatgttcca gaagcagaaa gccgccggat accagcgcgt tacgcgactg
     2581 ttcgatgata tgcacgatgt ctgccattcc actgtctgcc gcaaccagcg cagtgagcgg
     2641 ctcaaagcgg acatcgcctt gttgaagatg tgggtcctgc tcgtcaatat acggcggatt
     2701 gctgacaatc atcgcaaact gctgcccggc tagcgcgcta aaccagtcgc tttgcagaat
     2761 gtggatattt ttgatcgcca gatgctgggc attacgttgt gccagggaga cagcatcagg
     2821 catacgatct acagcgataa tttcgcagtc cgggcgctcg ctagccagcg caagcgcaat
     2881 cgccccggta cccgtcccga gatcaagaat acggcaaggt tgttcaggca accgcgccag
     2941 tgcctgctcc accagacact ccgtatccgg gcgcggaatt aaggtcgctg gcgaaacaaa
     3001 taacggcaac gaccagaatt ctcgcacccc ggttaaatga gcaatgggtt caccatcgcg
     3061 acgacgtgtc agtagcgcat caagttgctg acattgttcg tcagtcagct gcgtttcacc
     3121 aaaggcgaga ataaaagtac gccctctgcc ggtaacatgc tccagcagga tttcagcatc
     3181 acgccgcggg ctttcgctcg cctgaagttg gcttattgct tcacgtaacc agtgttgata
     3241 ttccattatt cctgctcgga caacgccgcc agttggtcgg cctgatgttc ctggataatc
     3301 ggttcaatca gcatatccag cttaccttcc atcacttcat ccaggcggta gagcgtcagg
     3361 ttgatgcggt gatcggtaac gcgcccctgc gggaaattgt aagtacggtt acggtcgctg
     3421 cgatcgccac tccccagcag gttacgacgg gtagacgctt cggcctgttg gcgttttgcc
     3481 atttcagcag cgtggatgcg agcaccgagc actgaaagcg ccttcgcttt gtttttatgt
     3541 tgtgaacgtt catcctgaca ttcaacaaca atcccggtcg gcaagtgagt aatacgaatt
     3601 gccgaatcgg tggtgttaac gtgctgacca cccgcccctg acgagcggaa agtatcaatg
     3661 cgtaaatctg ctgggttgat gtccggcagt tctgcgtcag gcagttctgg cattaccgca
     3721 acggtacaag cagaagtatg aatacgaccc tgcgattccg tagcaggaac acgctgcacg
     3781 cgatgaccgc cagattcaaa tttcagacga ccatacacac catcaccgct aattttggcg
     3841 atgatctctt tataaccacc atgttcaccc tcgctggcgc tcatgatttc tacccgccag
     3901 cggcgggctt cggcgtaacg gctgtacata cggaacagat cgcctgcgaa cagcgccgct
     3961 tcgtcgccgc cggttccggc tcggacttcg aggaaggcgt tacgttcgtc atcaggatct
     4021 tttggcagta acagaacctg taattgctgt tccagttgct cgcttttttc tttagctttg
     4081 cgcagttcat cctgcgccat ctcacgcatt tcaggatcat cgagcatcat ctgtgcggtt
     4141 tcgatatctt cctgaacctg ttgccagtcg gtaaaacagc gcgaaacatc acttaactgc
     4201 gcatattcgc gtgataatgc gcgaaaacgt tcctggtcgg cgatagtttg cgcgtcaccc
     4261 agcaacgcct gaacttcttc atggcgttca tgcagggctt ccagtttggc aacgatagaa
     4321 ggcttcatag gcgtaaatgc accctgtaaa aaaagaaaat gatgtactgc tactccagcc
     4381 cgaggctgtc gcgcagaata ttcaggcgtt cgttatcccc gtcacgggcg gcctgttgaa
     4441 gtgatttcgt tggcgcatgg atcaagcggt tagtcagttt ccatgccaga tcctgcataa
     4501 tggcttgcgc gtcgccgccc tgctcaaggg ccgctaacgc tttggcggtt aactcatcgc
     4561 gaacgtgctc tgcctggctg cgatactcgc gaatggtttc gctggcgctt tgtgctcgca
     4621 gccacgccat aaattcgctg gtttcctgag cgacaatagt ttccgcctca accgctgcgg
     4681 ctttacgctg cgccaggttg tgcgaaatga tgctttgcag atcgtccaca ctataaagat
     4741 aagcattcgc cagtttgcca acttccggct caacatcgcg cggaacggca atatccacca
     4801 acagcattgg ttggttacga cgacttttta atgcgcgctc caccatgcct ttcccgataa
     4861 tcggtaacgg gctggcggtg gaactgatga tgatatcggc ttcgcgcaga cgttcgtcga
     4921 tctcactcag ggcaatcact tccgcgccaa cttcatctgc cagaatttgg gcacgttcgc
     4981 gagtgcggtt ggcgataatc atcttctgta ctttatgttc gcgcagatga cgcgctacca
     5041 gctcgatggt ttcgcccgcg cctaccagca acactgtgac cgtagagagc gattcaaaga
     5101 tctgccgcgc cagcgtacaa gccgcaaaag cgacagacac agcactggca ccgatatctg
     5161 tttcagtgcg aacgcgtttc gctacagaga aagatttctg gaacatgcgt tccagttcgc
     5221 tggccttcat atgacctttt tgcgaatcgg caaacgcttt tttaacctga ccgaggatct
     5281 gcggctcccc aagaaccagt gaatccaggc cgctggcaac acgcattaaa tggctaaccg
     5341 cgtcgttatc ctgatgccag tagaggcttt tacgcagatc ttcttcatta agattgtgat
     5401 aatcgcaaag ccagcggatt aacgcctctt gcaggttatc ctgctcttca acgctaagat
     5461 aaagttccgt gcggttgcac gtcgacagca ccacgccgcc ctgcaccatc ggctgcgcaa
     5521 gcaggctgtc aagcgcctga tcgagcttat ccggcgaaaa cgatacacgt tctcgcagcg
     5581 atacaggtgc cgttttatgg ttgataccga gtgctaaaag ggtcatgtct gcgggaaata
     5641 ataccaacgt tgatagggtt agtctgcttg catcatacag gatgcgtaag atcaataaaa
     5701 gagagcgccc ccttttggag taattgccga aagccgttag attctggcaa ttaagacaac
     5761 ttgaacatag acgatagcgg acggtaacgc tagcattaag ggttataact gcaacgtatc
     5821 tcaaggactt gtcatcacta tgcccctgcc cgattttcgt cttatccgcc tgctaccgct
     5881 ggctgctctt gtgctcactg cctgttccgt taccacgccc aaaggtcctg gcaaaagccc
     5941 ggattcgcca caatggcgtc agcatcagca agacgtgcgc aatcttaatc agtatcagac
     6001 tcgcggcgcg ttcgcttata tttctgacca acaaaaagtg tacgcccgct ttttctggca
     6061 gcaaaccggc caggatcgct accgtctgct gctcactaac ccattgggca gcacggaact
     6121 ggagctgaat gctcaaccgg gtaacgtgca gttagtcgac aataaaggtc agcgttatac
     6181 cgccgatgac gccgaagaga tgattggcaa attgaccgga atgccaattc cgctcaacag
     6241 cttgcgccag tggattttag gtttaccggg tgatgcaacc gactacaaac tggacgacca
     6301 gtaccgcctg agcgaaatta cctacagcca gaatggcaaa aactggaagg ttgtttatgg
     6361 tggttatgac accaaaacgc atcctgcgat gccagccaat atggaactca ccgacggtgg
     6421 tcaacgcatc aagttaaaaa tggataactg gatagtgaaa taatgcggac acagtggccc
     6481 tctccggcaa aacttaatct gtttttatac attaccggtc agcgtgcgga tggttaccac
     6541 acgctgcaaa cgctgtttca gtttcttgat tacggcgaca ccatcagcat tgagcttcgt
     6601 gacgatgggg atattcgtct gttaacgccc gttgaaggcg tggaacatga agataacctg
     6661 atcgttcgcg cagcgcgatt gttgatgaaa actgcggcag acagcgggcg tcttccgacg
     6721 ggaagcggtg cgaatatcag cattgacaag cgtttgccga tgggcggcgg tctcggcggt
     6781 ggttcatcca atgccgcgac ggtcctggtg gcattaaatc atctctggca atgcgggcta
     6841 agcatggatg agctggcgga aatggggctg acgctgggcg cagatgttcc tgtctttgtt
     6901 cgggggcatg ccgcgtttgc cgaaggcgtt ggtgaaatac taacgccggt ggatccgcca
     6961 gagaagtggt atctggtggc gcaccctggt gtaagtattc cgactccggt gatttttaaa
     7021 gatcctgaac tcccgcgcaa tacgccaaaa aggtcaatag aaacgttgct aaaatgtgaa
     7081 ttcagcaatg attgcgaggt tatcgcaaga aaacgttttc gcgaggttga tgcggtgctt
     7141 tcctggctgt tagaatacgc cccgtcgcgc ctgactggga caggggcctg tgtctttgct
     7201 gaatttgata cagagtctga agcccgccag gtgctagagc aagccccgga atggctcaat
     7261 ggctttgtgg cgaaaggcgt taatctttcc ccattgcaca gagccatgct ttaagccggg
     7321 caagctgagt ttcggtgaca acgtcacctt gttccagacg ttgcatcgcg ctctttaata
     7381 caccgcctgg aaaggatcat gcctggcccg cacagttttc ggcagattct ttccaccaat
     7441 ggacgcatgc ctgaggttct tctcgtgcct gatatgaagc tttttgctgg taacgccacc
     7501 ccggaactag cacaacgtat tgccaaccgc ctgtacactt cactcggcga cgccgctgta
     7561 ggtcgcttta gcgatggcga agtcagcgta caaattaatg aaaatgtacg cggtggtgat
     7621 attttcatca tccagtccac ttgtgcccct actaacgaca acctgatgga attagtcgtt
     7681 atggttgatg ccctgcgtcg tgcttccgca ggtcgtatca ccgctgttat cccctacttt
     7741 ggctatgcgc gccaggaccg tcgcgtccgt tccgctcgtg taccaatcac tgcgaaagtg
     7801 gttgcagact tcctctccag cgtcggtgtt gaccgtgtgc tgacagtgga tctgcacgct
     7861 gaacagattc agggtttctt cgacgttccg gttgataacg tatttggtag cccgatcctg
     7921 ctggaagaca tgctgcagct gaatctggat aacccaatgg tggtttctcc ggacatcggc
     7981 ggcgttgtgc gtgcccgcgc tatcgctaag ctgctgaacg ataccgatat ggcaatcatc
     8041 gacaaacgtc gtccgcgtgc gaacgtttcc caggtgatgc atatcatcgg tgacgttgca
     8101 ggtcgtgact gcgtactggt cgatgatatg atcgacactg gcggtacgct gtgtaaagct
     8161 gctgaagctc tgaaagaacg tggtgctaaa cgtgtatttg cgtacgcgac tcacccgatc
     8221 ttctctggca acgcggcgaa caacctgcgt aactctgtaa ttgatgaagt cgttgtctgc
     8281 gataccattc cgctgagcga tgaaatcaaa tcactgccga acgtgcgtac tctgaccctg
     8341 tcaggtatgc tggccgaagc gattcgtcgt atcagcaacg aagaatcgat ctctgccatg
     8401 ttcgaacact aatcgaaccc ggctcaaaga cccgctgcgg cgggtttttt tgtctgtaat
     8461 atccatttgt atgacctatg cctccttcac ctgccattta gttgacagat gatgcgctca
     8521 cggatgaaac attattgtga acaaaatatt ttcctcacat gtgatgcctt tccgcgctct
     8581 gatcgacgct tgctggaaag aaaaatatac tgccgcacgg tttacccgtg acctgattgc
     8641 cgggataacc gtcgggatta ttgctatccc gctggcgatg gcgttggcta ttggtagtgg
     8701 tgtggcaccc cagtacggtt tatataccgc agctgttgcg gggattgtca ttgctctgac
     8761 gggtgggtca cgctttagcg tttccggtcc gactgcggca tttgtggtaa ttctctatcc
     8821 cgtgtcgcaa cagtttggac tggcaggact gctggttgcg gccttgctgt cggggatctt
     8881 tttgattctg atgggtctgg cacgctttgg tcgcctgatt gagtatattc cggtttccgt
     8941 caccttaggt ttcacctcgg gtatcgggat caccatcggt accatgcaga ttaaagattt
     9001 tctcggtctg caaatggccc atgtcccgga acattatcta caaaaagtcg gcgcattatt
     9061 tatggcgctg ccgaccatta atgtgggtga tgctgccatt ggcattgtga cgctaggtat
     9121 tcttgttttt tggccgcgtc tgggcattcg tttacccggt caccttccgg ccttgctggc
     9181 tggttgcgcg gtgatgggga ttgttaacct gctcggcgga catgttgcta ccatcggttc
     9241 gcaattccac tacgtcctgg ccgatggttc tcagggtaac ggtattccgc aactgctgcc
     9301 gcaactggtg ctgccgtggg atctgcctaa ttcagaattc acgctaacct gggattctat
     9361 tcgcacactg ctgcctgcgg cattctcaat ggcaatgctc ggcgcaatcg aatctctgct
     9421 ctgcgccgtg gtactggatg gtatgaccgg gacgaaacac aaggcgaaca gcgaactggt
     9481 tggacaggga ctggggaata ttatcgctcc attctttggt ggtattaccg ctacagctgc
     9541 catcgcgcgt tctgccgcta acgtccgtgc cggggcaact tcccctatct cagcggtgat
     9601 ccactctatt ctggttattc ttgccctgct ggtactggca ccgctgctct cctggctgcc
     9661 gctttccgct atggcagccc tgctgttgat ggtggcgtgg aacatgagtg aagcgcataa
     9721 agtggtcgac ttgctgcgtc atgcaccgaa agatgacatc attgtcatgc tgctgtgcat
     9781 gtcgctgacc gtgctgtttg atatggttat tgccatcagc gtggggatcg tgctggcatc
     9841 gctgctgttt atgcgtcgta tcgcacgtat gactcgcctg gcaccggtag tcgtagatgt
     9901 tccagacgat gttctggtac tgcgcgttat tggcccgctg ttttttgctg ctgctgaagg
     9961 cttgttcacg gacctggagt cacgtcttga aggcaaacgg attgtgattc tgaagtggga
    10021 tgccgttccg gtacttgatg ctggtggtct tgatgcgttc cagcgttttg tgaagcgtct
    10081 gcccgaagga tgtgaactgc gcgtgtgcaa cgtggaattc cagccactgc gcactatggc
    10141 tcgcgcaggc attcaaccga tcccgggacg cctcgcgttc ttcccgaatc gtcgcgcggc
    10201 gatggcggat ttataagtac gagattgacc agtcagcgca aactgactgg tcagcaaact
    10261 gcattatttg ttagctatga cggcgattat cgctacggcg gcaacgtttg tcatagtggc
    10321 gaacccacca gtaacggtcg cacacctgtt catgcccgcc aacacgggca cccgccagcc
    10381 agagaatggc accgacgaaa atacttagca ctgcaccatg tgcgaaatac tggggcatat
    10441 taaactgtgg taactggttg aggattgaat accccacgcc gaccaccatt accaccagac
    10501 ccaaccccat gagcacgtta ccgagtaacg aagcgttttt gcgtttcata tgtcacctcc
    10561 ggaactttct gggttgtaac agggaatacc cctcttcctt atgtgtaaag tatagacaac
    10621 accctgtggg ttatgtgcgg gcgtgatcac aattacaacc cttatttcaa caaaacttta
    10681 caaataaacg cctgaacacc ttgactttct aatagtccac tcaggcaatt attacgttat
    10741 ctgcatttga tacgcagttt tttgtcaagc ggggccgcaa ccagtaaact acgcgccagt
    10801 tatgtacaca ctcaggacaa aaaaacgtga cgattaaatt gattgtcggc ctggcgaacc
    10861 ccggtgctga atacgccgca acgcgacata atgctggtgc ctggttcgtt gacttactgg
    10921 cagagcgttt gcgcgctccg ctgcgcgaag aggctaaatt ctttggttat acttcgcgag
    10981 tcactcttgg aggcgaagat gtccgcctgt tagtcccgac tacatttatg aatctcagcg
    11041 gcaaagccgt tgcggcgatg gccagttttt tccgcattaa tccggacgaa attctggtgg
    11101 cccacgacga actggatctg cctcctggcg tcgccaaatt taaattgggc ggtggccatg
    11161 gtggtcacaa tggactgaaa gacatcatca gtaaattggg taataaccct aactttcacc
    11221 gtttacgcat cggaatcggt catccgggcg ataaaaataa agttgtcggt tttgtgttag
    11281 gcaaaccgcc tgttagtgaa cagaagttaa ttgatgaagc cattgacgaa gcggcgcgtt
    11341 gtactgaaat gtggtttaca gatggcttga ccaaagcaac gaaccgattg cacgccttta
    11401 aagcgcaata agtcgttgtc tgcggcattt ttgccgagtg ccgtgtataa taggcaaagt
    11461 tatttccatt tctgcaatct gttagcaata acaggttgat tattaagata ttaaggtgat
    11521 ttaaatcatg ggattcaaat gcggtatcgt cggtttgccc aacgtcggga aatctaccct
    11581 gttcaacgcg ctgaccaaag ccggtattga agcggccaac tttccattct gcaccattga
    11641 gccgaacaca ggcgtcgtac caatgcctga tcctcgcctg gatcaactgg ctgaaatcgt
    11701 aaaaccgcag cgtacgcttc ccacgaccat ggaatttgtc gatatcgccg gtctggtaaa
    11761 aggcgcatcg aaaggcgaag gtctgggtaa ccagttcctg accaacatcc gtgaaaccga
    11821 agcgatcggt cacgttgttc gctgctttga aaatgacaac atcattcacg tttccggcaa
    11881 agttaacccg gctgacgata ttgaagttat caacaccgaa ctggcgctgg cagacctcga
    11941 cacctgcgaa cgtgcgattc atcgcgtaca gaagaaagcc aaaggtggcg ataaagacgc
    12001 gaaagctgag ctggcggtcc tggaaaaatg cctgccccag ttggaaaatg caggtatgct
    12061 gcgcgcgctg gatttaagcg ctgaagagaa agcggctatt cgttacctga gcttcctgac
    12121 gctgaaacca acaatgtaca tcgccaacgt caacgaagac ggttttgaaa acaacccata
    12181 tcttgaccag gtgcgtgaaa tcgcggcgaa agaaggttct gttgtggttc cggtttgtgc
    12241 tgctgttgaa gcagacattg ccgaactgga cgacgaagaa cgtgacgagt ttatgcagga
    12301 gcttgggctg gaagagccgg gcctgaaccg tgtgatccgt gccggttata agctgctgaa
    12361 cctgcaaact tacttcaccg ctggggtgaa agaagtgcgt gcatggacca ttccggttgg
    12421 agcaaccgcg ccgcaggcag cgggcaaaat ccatactgat tttgaaaaag gctttatccg
    12481 tgcacaaacc atctcgtttg aagatttcat cacttacaaa ggtgaacaag gcgcgaaaga
    12541 agcaggcaaa atgcgtgcag aaggtaaaga ttacatcgtg aaagatggcg atgtgatgaa
    12601 cttccttttc aacgtctaat taatttattg tctcatgagg tttcgctaaa tctcatgaag
    12661 agcgcaaaag tatataaaat ccacgcaatt gcgtggattt ttcatttcac cgagtcctaa
    12721 tcattcttgt aacatcctac acttttgtgc ttattgccta tagtgaacca cgagaataat
    12781 taaagacata agtgttcaga tgacggatat ctcattagca gtgatataac gtaagttttt
    12841 gtatcactac acatcagcca ccttcacagc tgatcaaaat tgatcacgct ccaatatgct
    12901 ggcaagatga aaatgataac cagtagcaca ataacaacga taggccagaa aacccattga
    12961 ccaaaaatga atatcgatgt tttttgcttt ttcttattag taatgcaaaa atggcgcata
    13021 ttgtttttgt tatgcagata catcaagcga aattcccata actaattagg attattacaa
    13081 aaaatcttca ccaaattttt taaccgctgt ttgttgtaat atttttgcca tatttatagt
    13141 aacgaattgg aataagtaac gacaacttag ggaataactc gatataaact cacctttgta
    13201 agggggatgt taaatatata ttaattcctg tgttcctgat tttgcccggt aatagagaat
    13261 tattctctga gaagctcatc atgattgcac catacaaata tcatgatgat aagaggatat
    13321 tattcattga tttaaacaaa atgacaaaat ccatagaccc atagtctggg tactatatag
    13381 gagtggtatg ggcatcaaac aacacaatgg gaataccaaa gcagatcgtc tcgctgaatt
    13441 aaaaatccgt tcgccctcaa ttcaactgat aaaatttggc gctattggtt tgaatgcaat
    13501 tatcttttcc cccctgctga tagctgctga tacaggaagt caatatggca ccaatattac
    13561 tattaatgat ggtgacagaa ttacaggaga taccgccgat ccatcaggaa acctctatgg
    13621 tgtaatgacc ccagcaggaa acacgcctgg caatatcaac ctgggtaatg atgtcaccgt
    13681 caatgtcaac gacgcctctg gatatgcaaa aggaatcatt attcagggca aaaacagctc
    13741 cctgacagct aaccgactca cagtagatgt tgttggtcaa acctctgcca tcggc
//
LOCUS       DAJTNA010000103        13678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTNA010000103 DAJTNA010000000
VERSION     DAJTNA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13678
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.104"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..1868)
                     /gene="speC"
                     /locus_tag="OO161_002583"
                     /pseudo
     CDS             complement(1..1868)
                     /gene="speC"
                     /locus_tag="OO161_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2266..2973
                     /locus_tag="OO161_002584"
     CDS             2266..2973
                     /locus_tag="OO161_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5463612.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3079..3154
                     /locus_tag="OO161_002585"
     tRNA            3079..3154
                     /locus_tag="OO161_002585"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3112..3114,aa:Phe,seq:gaa)
     gene            complement(3303..3839)
                     /gene="yghD"
                     /locus_tag="OO161_002586"
     CDS             complement(3303..3839)
                     /gene="yghD"
                     /locus_tag="OO161_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCR5463613.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(3841..5019)
                     /gene="gspL"
                     /locus_tag="OO161_002587"
     CDS             complement(3841..5019)
                     /gene="gspL"
                     /locus_tag="OO161_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCR5463614.1"
                     /translation="MSSILEIFFPLCATDPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5016..6026)
                     /gene="gspK"
                     /locus_tag="OO161_002588"
     CDS             complement(5016..6026)
                     /gene="gspK"
                     /locus_tag="OO161_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCR5463615.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQ
                     GQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWVF
                     IDEDRSVQTRLGREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCA
                     LPMIRQQININTLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLL
                     ADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGE
                     SE"
     gene            complement(6029..6628)
                     /gene="gspJ"
                     /locus_tag="OO161_002589"
     CDS             complement(6029..6628)
                     /gene="gspJ"
                     /locus_tag="OO161_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCR5463616.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6625..6996)
                     /gene="gspI"
                     /locus_tag="OO161_002590"
     CDS             complement(6625..6996)
                     /gene="gspI"
                     /locus_tag="OO161_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCR5463617.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(6993..7556)
                     /gene="gspH"
                     /locus_tag="OO161_002591"
     CDS             complement(6993..7556)
                     /gene="gspH"
                     /locus_tag="OO161_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCR5463618.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(7560..8015)
                     /gene="gspG"
                     /locus_tag="OO161_002592"
     CDS             complement(7560..8015)
                     /gene="gspG"
                     /locus_tag="OO161_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCR5463619.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(8032..9255)
                     /gene="gspF"
                     /locus_tag="OO161_002593"
     CDS             complement(8032..9255)
                     /gene="gspF"
                     /locus_tag="OO161_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCR5463620.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9255..10747)
                     /gene="gspE"
                     /locus_tag="OO161_002594"
                     /pseudo
     CDS             complement(9255..10747)
                     /gene="gspE"
                     /locus_tag="OO161_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10747..12807)
                     /gene="gspD"
                     /locus_tag="OO161_002595"
                     /pseudo
     CDS             complement(10747..12807)
                     /gene="gspD"
                     /locus_tag="OO161_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12837..13667)
                     /gene="gspC"
                     /locus_tag="OO161_002596"
     CDS             complement(12837..13667)
                     /gene="gspC"
                     /locus_tag="OO161_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HCR5463621.1"
                     /translation="MFWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKT
                     FDKNDVQLISQQNWFGKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEG
                     GKQQVYLQGETLGSHNAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAATNKKAVSD
                     EAKQAVAEPAVSAPVEIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRS
                     LFDASGFKEGDIAIALNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR
                     "
BASE COUNT         3062 a         3950 c         3532 g         3134 t
ORIGIN      
        1 cagcgctatg cgcatcctgg gggttcatca ccactgacgg gaaactctgc tggcggaaca
       61 tcgctttttg taggtctttg acgtcgaaac tgacatacag atcgtgcatc tcctgacaca
      121 actggcgcaa agtgtagtcg cgatagcgca ccggatattt gttataaacg ctcggcaaca
      181 cctcagccag cggcgagtca tcctcaatat gctgttcaaa ctgcgccaac atctctacca
      241 gttgtgccag cttctcgtgg ctttccgccg gagttaataa gaacagaatg gagttgagat
      301 cgcacttctc cggcacaatg ccgttctcac gcagatagtg cgccagaatc gtcgccggaa
      361 cgccaaagtc gctatattcg ccggtttcgg cattgatacc tggtgtagtg agtaacagct
      421 tgcacggatc aacaaaatac tgatccgcgg catatccttc aaagccgtgc cacttcgccc
      481 ccggctcaaa actgaaaaaa cggcggtcgc tggctaacac tgatgtcgga taatcctgcc
      541 acaatttgcc atcaacaacg ggcgggataa acgggcggaa cagcttacag cgcgcaagaa
      601 tagccttgcg cgactcaata cccaactcaa cgcactcagc ccacagccga cgcccactct
      661 ccccttcatg aattttggcg ttaacatcca gtgcagcaaa cagcggatag aaagggctgg
      721 tagaagcatg gagcataaag gcgttattca accgcttatg cgggcaaaaa cgcgcctgtc
      781 cgcggatatg gttatctttt ttatggatct gcgacgtctg tgagaatccc gcctgctgtt
      841 tgtgcaccga ctgagtcaca aagatccccg gatcgttttc gttaagttct aatagcagcg
      901 gcgagctatc cgccatcatt gggataaact gttcgtaacc gacccacgcg gaatcaaaca
      961 gaatgtaatc acacagatgc ccaacggtat cgatcacctg acgggcgtta tagacagttc
     1021 cgtcataggt tcccagctga ataatcgcca ggcgaaacgg gcgcggcagc tcggcttttt
     1081 ctggcgcaac gtcgcgaatt tgctggcgca gatactcttc attaaaacag tgcgcatcaa
     1141 taccgccaat gaaaccaaac gggttgcgtg aagcttccag atagaccggc gtcgcccccg
     1201 cctgaatcag cgcgccgtga tgattcgact tatggttgtt acggtcgaag agcaccagat
     1261 cgccacgcgt taacagcgca ttcgtcacca ctttattcgc tgccgatgtg ccgttcagca
     1321 caaaataggt tttatcggca tgaaagactt tggctgcgaa tttctgcgca tctttcgcca
     1381 ataagcagat cgcccaattt tacgtcagcg ttacacatat cggcgcgaaa gacgttctca
     1441 ccaaagaaat cgtaaaaatg gcgtccggca ggatgctttt taaaaaacgc accatgttga
     1501 tgtccagggc aagcaaaggt gctgttgccc atctcaacgt actgcgtcag cgtgtcataa
     1561 aacggtggca gcaaattctc ttcatactga caggctgcgg attccagctc cagccactgc
     1621 tgctcgttgc cgttgattac cgccgtaacg cccgcaggta attcaacagc atgttcggaa
     1681 tacaaaaaca ccggtagatg aaaaccggtg cgcttaagca acgcaagaat gccactgcga
     1741 ctatccgcag cggtaatgac gactgccgcg acgtccgtaa aatcagtatc tcccaacgcc
     1801 accacgcgac gatgagaaga aagtcgggat accagttcac tactggcggc aatattcatt
     1861 gatttcataa gcgcaaaccc gtttcgggga agtaagaata ccggacaagg tggaaaaccc
     1921 tgccccatga gatatggggc aaactggtca ccagctccga ccgccagaca tcagtaaaag
     1981 cagaaacgct ctgattttac tgttgtcctg cagtgagcgt gcgttaactt caccgcatga
     2041 gcagtaacat agaaagggaa acgtttcgcg cgaaacggcg ataagcgaga gaatgtaagg
     2101 aggtggcgtg catcgggcaa actccgtaca gagaggagaa aattcgcgca atcatggcac
     2161 ctttcgctaa agcgtgcaag ccaggacttt gcgaccagac aagccatcaa caaggcaaaa
     2221 agttcataat aagaaaatca aacaatacag ttaacaggaa ctcttgtggt catcggccct
     2281 tttatcaacg ctagtgcagt cttactgggt ggcgttctcg gcgcactgct cagccaacgc
     2341 ttaccggaac gtatccgcat ctccatgaca tctatttttg gtctggcatc gctggggatt
     2401 ggtattttac tggttgtgaa atgcgccaac cttccagcga tggttttagc tacgctactt
     2461 ggcgctctaa ttggcgaaat ttgtttgctg gagaaaggtg tcaatacagc agtcgccaaa
     2521 gcacaaaatc tgtttcgtca ctcacgtaag aagccaacgc atgaatcttt tattcagaat
     2581 tatgtcgcga ttattgtcct gttttgcgcc agcggcaccg ggatcttcgg ggcgatgaac
     2641 gaagggatga ccggcgatcc gagtatttta atcgccaagt catttcttga tttctttacg
     2701 gcgatgatct tcgcctgctc gctgggtatt gcggtgtcgg taattagtat cccattactg
     2761 atcatccagt taacgctggc gtgggctgcc gcgctgatat taccgctgac cacaccgtcg
     2821 atgatggcag acttcagcgc cgtaggcggt ttattgctgc tggcaaccgg attacgcatc
     2881 tgtggcatta aaatgttccc ggtggtcaac atgcttccag cactccttct ggcaatgccg
     2941 ctttccgccg cctggaccgc ttggtttgcc tgacaatgcg tgcaatagcg gcaaagtgat
     3001 gatagattgt gcagtctgca gtaaattgaa gaaatttgat tgacgagacg aggcgaatca
     3061 ggtttaatgc gccccgttgc ccggatagct cagtcggtag agcaggggat tgaaaatccc
     3121 cgtgtccttg gttcgattcc gagtccgggc accactaatt cttaagaacc cgcccacaag
     3181 gcgggttttt gcttttggat ctgacaataa ccttcacgaa aaaagtttgc ttataaagtc
     3241 tgggggaatt actctcgcca cgttaacgag agtaatttta ttgatactaa tctcctgata
     3301 ctttaccccc gtccaaactc cagccgctgc acattcacca tcccagtctt ctcagcagca
     3361 ctggcatcaa tttgtgtcac ccgcagcgca tacttttcat ccagcgcgtt taaccagttc
     3421 agcaggtcat taaacaccac cggatctatc cagacctgaa tattctcccc acgctcggct
     3481 atccgcttga tgactaccga gtgcgcggaa gcactgtcac tgataacccg cgatacctgc
     3541 gcaggcgttg ttgtggcgga ttttcgcgcc gcaataatat ccggcgcggc gctcctcagt
     3601 ctggtgttca tcgccaccag ttgctgcaac attgtctcct gttgtactat ccgttcgctc
     3661 aacggctgcc agatgaggac gtaatatccg acgctaaaca ggaacaccac cgctgccagt
     3721 aacatgccct tttcacgcgg agaacgcccc gctaggtgtt gtgccagcca gtgttcgcca
     3781 cggcttaact ggcgttcacg ccattgctga aaatagtgaa taaatttatc gcgtaacatg
     3841 ttatttcctc cgcaacgtta cgccgccgga aaccgcatca ccctctttct gtaacgcgtc
     3901 ctgttgcaca acataatcgg ttgccagtgc actacgcagt ttgtcgaaac tggcaaagtt
     3961 cgcggcccgt agctggaggt gaagtatctg gcgtttttga tcaaaggtaa aaccacgcat
     4021 ttcgatgtcg gaaagtgacg cagatttcag ggtgctggct atcgctgaca actcggcgag
     4081 cagccgggta tcgtcggcct gtgggcgata ttttttcagc gccatcgtca cctgagagcg
     4141 taaattcaca atccgcttct gctccgggaa caacgttaag aactgttcct ccgcctgggt
     4201 gcggctttgc gccacctgtt cgctgacgct ccataacgtc acgccccgct ccactgccag
     4261 cgcaactaga atcagcaata tcggcagaat catcacccgc cagcgcgccc actgttttcg
     4321 gtagctgacg cgaggctgcc acggccctgt cagcaggttc ccttccggtt cgccataagt
     4381 ggtaacggcg ggcagagctg taacggtcag gcgttcagcg tctgcgccag cccatgctga
     4441 tagcttttcc ggtgcaatgc cgactacggt tagcgaaagc ggtaaatcct gctcattgag
     4501 ctgggcgcgg aacatgaccg gagccagcgc ccgcccggcg ctccatcccc ggcattcatc
     4561 aatgcggcag ataacccgtt gcgcatcgcc agccataacc ccacaaggaa tggacatcca
     4621 gtctggcgcg acggtagcgc gggtgatgcc gttttcctgc aaccactgcg caatgttgcg
     4681 catatgctgt tggtgaatca ctgctacggt tgccagttgc tggtcgatat tcaacggggc
     4741 gaaatgcagt tcatcgatat cctggttcag ctcttcttcc agtaaggcgg gcagaatggt
     4801 cggtatctgc ttacgggcga catcaggcag ttcaacctgc cagacgctga tccattcgcc
     4861 gggaatgtag agtcgaatcg catcggtttg cagccattgc tggagctgtt cgttagcaac
     4921 gtcagaccag ataccgtgct ccacatcggg tgtacggcgc tgccaatgga tgggatcagt
     4981 ggcgcaaagc gggaaaaaaa tctcaaggat ggaactcact cactttctcc tgtctgatgc
     5041 cagagaaccg aaaagtgttg tgggcccatg cggacaatta acgagttcat cgtcagttca
     5101 atctcattca cggtgatatc tgaacgcagc cagaagtaat tgctgtccac gctcaggacg
     5161 gtttttagct gttttttagt acgctcatcg acgtcagcaa gtagcggctg tgcaagaaac
     5221 tgatcgacat cttcccagcc cttcgccgga cgttgttgta ataacgcccg cgcctgaacg
     5281 gggcttaacc acgggtcaaa cagcgcctca agaatcacac tttgcgtgac gtctaaagta
     5341 ttgatgttga tttgctggcg gatcatcggc agcgcacaga ccagcggttt cagtttttga
     5401 taaagcccgg cgtccattcc ctgtaccacg cgcatctcac tgatatcagc cagcggttga
     5461 ttggcggcgt agaacggcac cgaacgggcg agatactcgc tgtcttcacg gcccagacgt
     5521 gtctgcacgc tgcggtcctc gtcaataaac acccacaggc tttcggctat cagttcggcc
     5581 cgataagcag gcacatctag gcgcgagatc agggcaatca actgttgtac cgcaagcgga
     5641 cgcgacgccg ttgtcggctg agcaagggca ttcaggttaa agcaagcctg tgcgtcacgc
     5701 agggtgaccg caatttgccc ctgcggcaga gggaagaaac gagggccgga tgcccacggc
     5761 tgtgccagat gcacgcgctt ttcatttttc aggctcagac tgagcgcgct cagcgccagg
     5821 ctttccgcac tggcgctgta ccacagcgcc aggctttccg cactggcgct gtaccacagc
     5881 gcctgctggt actcctgctg gctgcgcgtt cgcccaagtt gttgctgcat ccgcccggaa
     5941 agcgtgatgg ttaccagcat cataaccgcc agcaatacca gcaccacgac cagtgccatt
     6001 ccgcgttttg gtggtgaggt gatcatgatt attgcggccc gcgtaacaac cagatgcgtt
     6061 caatctcgcc ccattgcggc gaatgcaggg tcatgcgtac cgccacaggg atcgcctgta
     6121 ctgacgacca gctctcctgc cagcgcgtgc cgtcgtagaa ctgcaaacgg agcgaatcgg
     6181 ccggaatcaa cttttgcatt gtcggcttca cgctgcctgc ggcatcggtc agcggccagg
     6241 ctaaccgttc gagataaccg tcatgaatgc ggtaaccgac ggtgagcaga ttgctgcgcg
     6301 gcagacgcat caacgggtta accacaccgc cgcgcacaaa acgcattcct tcactctcag
     6361 acgccagcac gccagcgcca gccagtaaag ctggttcacg ctggccctga tcgcctctta
     6421 ccggacgcgg catcatttgg gtcagatcgt gggtcagaaa actcatcgtt tgctgcatga
     6481 ggtttagttt ttgatcgtga tccgcgacgg cgctattcac gcgcgtgacg ccgttagtca
     6541 cctgctgcgc catcagtgcc agcgaggcaa aaatggcaat tgccaccaac atttccagta
     6601 acgtgaaacc agcgcgagtc cttctcactg ttggcctccc acggcgctaa accaggcgcg
     6661 tcgtgactga atcaccgacg aaaagtcttc gtgacggctg acttcaatat ccaccgcctg
     6721 gagcagcgcg ttgcctgtat tcaatgggat tgttcgccag taccagcggc ttcctgccat
     6781 atcgctctcg ccctgatgcc tgaccgcccg ctgctcacgg gtcatcaggt agagcagcgc
     6841 agtctggttt tcagctaccc agcccgctac cgttttttcc tcaagaacgt gctgattact
     6901 cagcgcaccg ctggcaatct gtaacaccgc cgtggcagcc agcgcaaaaa tcgccagcac
     6961 gagcatcact tccagcaagg taaatccgcg cttcatggca tcctctcatc acattgattg
     7021 agggataacg cgccatcgtg tgccagcttt accgcccaac atgcgttttg cgccgccgag
     7081 tagaagcgca gcgtaaacgg cgtggcgggt tcaaaaggcg aaaaacggat ctgcggcgtc
     7141 ttctttttcg cctctttttg caactccagt tcaatatcgt gcagcgtcag gcgacgacgt
     7201 tgcagctcca gcgcatattc cttctgccag atatcacttc cgggttgtaa cagcatctgc
     7261 acctgtttcg gcaccgtaac ctgtgccgat aaccgggtcg cagaaacggg caaccactgc
     7321 ccctgacgac gctgcataaa ctgatagcca ggcgggtcga ttagcacacc gagtgtctgt
     7381 ccttcaatca ctgccctgtc cttaaactgc gcaaagcgag tcagaaaatc ctgtgccgct
     7441 tttttcgcgg gcggttcaga atcggtcgca aacgtctgta ccacgcccgc actggcaagg
     7501 ccgataagga aaatcaccag catgatttcc agaagtgtga atccgcgttc aggcactgct
     7561 tactgaaact cctgcaaatt ccagttaccg atatctgcgc cagcgccttc cccgttttcc
     7621 tgaccatccg cccccagggt ataaacatca aacagtcctt tttcacccgg gctgagatac
     7681 tgataatcat tgccccacgg atcctttggc agtcgtttga tgtatccacc agtacggtag
     7741 ttgcgggagt ccgccatatt ggctggctgc tggatcagcg cttccagccc ctgttctgtg
     7801 gtcggataac gcccgttatc cagtcggtac atatctagtg cgttttccag cgccacgata
     7861 tcgctgatgg ctttttgccg atcggctttc tctttgttgc ccaacaggtt aggcaccacc
     7921 agacttgcca ggacgccaag aataacaatc accaccatca cttccagcag ggtaaaacct
     7981 gcccgtggtt tgtgtgtgcg ggataacgaa ttcatgtgat aactccgtaa attacattcc
     8041 aaccatatta ttcagttgca gcatcggctc gaggatggcg atgacaataa acagcaccac
     8101 gcccgccatc atcaccacca gcgcaggctc aaacagcccc aacgccagcc ccacctgggt
     8161 atcaaattcc cgttcctggt taacagcggc ctgctcaagc atggtttcca gttcgccgct
     8221 ctgttcgccg gaagcgatca tgtacagcat catcggcggg aacaggcgca actccgccag
     8281 cgcggcgcgc agactgcttc cttcgcggac gcgatctgcc gccagcagca gttgttgttc
     8341 gacatagcga tttgccgaca cggcggcagc ggtctgaatg ccttccagta gcggaacact
     8401 gctggcggtg aggatgctta atgtgcggga aaaacgcgcc gtattcagcc cgcgcgcaac
     8461 acggcccgtc acgggcagac gcagcaaggt tttatcccag cgcaggcgca tagtaggatt
     8521 tttaagtagc cattgcccca gcaccagaag cgccagcaat cccgccagcc agtaaacgcc
     8581 gctggcctgt aacgcatcac tcatggcgat aagcgcacgg gtggaggagg gtagcgcgtg
     8641 tccgagatga tcaaactgtt cgatgatttt cggcaccact gccgtcagca aaatagtgac
     8701 cacgcccgtt gccaccacca gcataaccag cggatagagc atggcctgca atagacgtga
     8761 tttcaggcgc tgtcgctgtt cggtgtaatc tgccaggcga ttgagcacca cgtcgagatg
     8821 cccggatttt tctccggcag cgaccatcga acaaaacagg gaatcaaaga cgcggggatg
     8881 ttcgcgcagg ctgtccgaca gggtataacc ttcctgaatc cggctgcgca gcgccattcc
     8941 gaggctcttc acatgcagtt tttcactttg ctcactgacc gcctgcaagc aggtttccaa
     9001 cggcattgct gcctgcacca gcgttgccag ttggcgcgtg aacagcgcca gatctgccgc
     9061 cgccacgcga cgatgtgcgt gccgccgacg ctgcaacatt ccccctgccg atgcattcat
     9121 cctggcttca atgtgcacgg ggataagctc tttaccgcgc aacaactggc gggcatgacg
     9181 cgcggaatcc gcctcaatca tgcctttggt tttgcgacca ttacgctcca gcgcctgata
     9241 gtaaaacagt gccattacgc ctccatggtt acccgcagaa cttcatcgag agaggtttct
     9301 ccggcgagca ctttctcaat gccgttgctg cggatacccg cagagtgttg ccggacataa
     9361 cgttccagct ccagctcccc ggcctggcgg tggatcaaat cacgcaatgt ggcatccacc
     9421 acgtcagctc atggatggca gtccgtccgc ggaaaccttt gtgattacag gcggggcagc
     9481 cctgcggatg gtacagagtg acggttcgcg catcggtaat gcccagcagg cgtttttctt
     9541 cgtcagtggc aggcgcggcc tgacggcagt cgggacacag cgtgcggacc agtcgctgcg
     9601 ccatcacgcc cgtcagactg gaagagagca ggaaaggctc cacgcccata tcctgcaaac
     9661 gcgtgatcgc ccccaccgcc gtgttggtat gcagcgtgga aaggaccagg tgtccggtaa
     9721 gcgatgcctg aacagcgatt tctgcggttt cggtatcgcg gatttcaccg accatcacca
     9781 catccgggtc ctgacgcaaa atcgcgcgca ggccacgggc gaaggtcatg ccgacgcggg
     9841 tgttaacctg tgtctgaccg atcccttcaa tcatgtattc gatggggtct tcaaccgtga
     9901 gaatgttacg cgaatggttg ttcagctcct gcaatccggc gtacagcgtg gtgcttttgc
     9961 cggaacccgt cggccccgtc accagaaaga tgccgtgcgg tttgtgtaac agctggcgca
    10021 actgcacggt cagttgctgg ctaagcccca gacgctccag cgtcaggcgg gcctggtttt
    10081 tgtccagcag tcgcagcacc acgcgctcgc cccaggcaga aggcatggtg gagacacgca
    10141 cgtcaatcgc ccggccgccc agcagcagcg caatacgtcc atcctgcggt acgcgctttt
    10201 cggcgatatc cagccgtgcc atcaccttga tacgcgatac cagcagcgag gccagtttgt
    10261 gcccagggcg cagcatttca tgtaatgtgc cgtcaacgcg aaaacggatc accagactct
    10321 tttcaaacgt ctcgatgtgg atatccgaag cgccttcttt aatcgcctct gccagcatgg
    10381 cgttgatcag tttgatgatc ggcgcatcgt cgtcactttc cagcagatct tccgtttctg
    10441 gcagttcttc cgcgagagta aaaaagtcct cagcagaacc gagatcttcc atcagctggc
    10501 gagcctcgga agagtcccgc tgccagaccg cattcagccg ctgttcaaat tcggcctcgt
    10561 cgatttgccg cagcgtaaag ggcgcgttca gcccccgctg cagctcctgc aaaacagaga
    10621 gcgatagcgg atgaacgtgg aggatctcca gcgacgcttc gcaccatgcc accaggctaa
    10681 accgacggct gaaactgtag ggcagacgca cggtgttggc ggtggtttcc tgtgctacag
    10741 gcaccattaa cgcgttctcc cggcattgag gaacgcgcga acttccggcg gtaaggcctg
    10801 gttttgcgct ggcagtaccg gctgtgcggt gtgcggcatc aggcttaaac cttgctcatc
    10861 acgatagatc tgttcggcgc gcatatagtt atatttgcgc tgcgacacgc cgtctgccgc
    10921 cataccgtca cgcagaatgg tcgggcggat aaacaccatc aggttacgtt tttctttttt
    10981 atccgccgtc gatttaaaca gtttaccaat caatgggata tcgcccagca gaggcacttt
    11041 cgccacgctt tctcccgcct gatcgtccat cagaccacca agcacaatca gctcaccatc
    11101 gttagccagc acggtggttt tcagtttgcg ctcaccaaac accacgtcga ggctggtctg
    11161 tccttccacc ttcgagactt cctgctcaat caccatctgt accgcgttac cttcgttaat
    11221 ctgcggcgtg actttcagca tgatgccgac ttttttcctt tctactgtat tgaaaggatt
    11281 gctgttattg gagccaacgg tagagccggt taataccgga acgtcctagc ccaccatgaa
    11341 gaaggcttcc tggttgtcca gcgtagtgat gctcggcgtg gagagaacgt tcgagctgga
    11401 gtcgttttta accgcctgca ccagtgccat ccagtcgcct ttcaccacgc caaccgccgt
    11461 accgctaaat ccagaaagaa gctgggcgag tgtggagaga tcgccgttgg tatccggatt
    11521 aatggtggta gcgccgtttt cactgatcac cgtcgagcct ttctgcggtt tcgcctgaga
    11581 aatggctgcc cccagcgtac cgatagggat ctgcgtacca ttagcaaact gcattaatcc
    11641 ggcatctttc gacgcccact gcacgccgaa gttgatattg ctaccttcgg caacttccac
    11701 gatcaacgcc tcgacgtgca cctgagcacg gcgaatatcc agttgttcaa ttacgctttg
    11761 cagcgactgc atgatgtcct gcggcgcggt tacaatcagg gcattactgt gtttgctggc
    11821 ggcgatggat acaacctcac gcccgctacc gaccgtgcct tccgcctctt ctttagccgc
    11881 cgtgagcgtg ccgctgacct gtttcagcac atcgaccaga tcttcggctt tgctgtattt
    11941 gagatagaaa acctggctgt tgccgctgcg ttccatttct gagtccagcc gacggatcag
    12001 gcggcgcatt ttgtcccgcg tggccgggtc accactgaca atcacactgt tggtgcgttc
    12061 gtcggcgaca atttgagatt tcagcgtcgc aggctggttc tcgccgctgt ttttcgtcag
    12121 gctttccagc acgcgggcga tttccgaagc cgaggcgtta tccagcggga tcacctcttc
    12181 ggtgcgatta cccgcgtgat ccacgcgctg gatcacttcc gtcaggcgct ccacaacgga
    12241 ggcgcgtccg gtgagcataa tcacgttgga gggatcgtaa ttaacaacgt tgcctgagcc
    12301 tgcgctgtcg atcatctggc gcagaatcgg tgccagctcg cgtaccgaca cattacgtac
    12361 cggcactact ttggtgacca tttcatcgcc cgcgtagttg tcgctgcctt caccgaccag
    12421 cggcagcggc tcgactttcg cagcgctgga tttcaccacc ttcagcacgt cgttttccat
    12481 cggtacgacg gcatacccct gtgcttccag caggttaagg aatagctggt aatactggcg
    12541 ttcattgagc ggggtcatgg tgcgaatact cactttcccc tgtacgcccg gccccataat
    12601 gatggtttta ttaaggttag cgccgacggt ttcgatgaac gatttcaggt cggtatcttt
    12661 aaaattagcg gtgaaagtgg cttcttccgc ccagaccggt gaactgcata atgccgctgc
    12721 cagcaccagc ggcagtaaac gcttttttgt tttgaggcca gttgtcttct tacgccagac
    12781 cgacaacgta atatcacgcc aaaacacgat gaatgattct cctggattaa atgcggttag
    12841 cgcagcgcga tggaaatgtc gtggcgcgca cccttgcgta aaaccgtaag ttgaatggaa
    12901 tccattgaag gtaactgccg catcagagca atcattgctc gtggatcagt gaaatcctgc
    12961 tgatttagcg caatggcgat atcgccttcc ttgaaaccgc tggcatcgaa cagagaacga
    13021 tctgcccccg gcttcactgc ataaccgaca attccctcct tacgcacagg cgtaagctgg
    13081 atatagttaa aaattttctg cggatctttc gccagtgcct gacgcacggc agtcggtatc
    13141 tcaactggcg cactgacagc aggttcagca acagcttgct ttgcttcgtc actgacagct
    13201 tttttgttgg tcgcggcaac ggtggaacgc tcctcttcag ccaggctcag gcgctctatt
    13261 tttccctgat agcgcagcat cacatggtcg cgattgattt cctcaatcac cgcgttgtgc
    13321 gagccaagcg tttcaccctg caaatagacc tgctgtttac cgccttcttc aataaccgcg
    13381 ccgggtctgg caccaaaggc gatcccgcgc agcaccacat taagacgcgt ttctgccaca
    13441 ggcacaggtt cgggttgttt taccggcgtg gcgacgggct gatatttgcc aaaccagttt
    13501 tgctggctga ttaattgcac gtcattttta tcgaacgttt ttgcatccgc acggggcggt
    13561 ttattcgccg atggggaaac agccgtatat tccgcagaaa aggagaaata gcgccagagt
    13621 gaatgcgcca cttttgcaga aataataagc agcatcagcc agaacatccc gcgtacaa
//
LOCUS       DAJTNA010000104        13620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTNA010000104 DAJTNA010000000
VERSION     DAJTNA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13620
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.105"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..1013
                     /locus_tag="OO161_002597"
                     /pseudo
     CDS             1..1013
                     /locus_tag="OO161_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            1196..1543
                     /locus_tag="OO161_002598"
     CDS             1196..1543
                     /locus_tag="OO161_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5463622.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            1533..1895
                     /locus_tag="OO161_002599"
     CDS             1533..1895
                     /locus_tag="OO161_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5463623.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            1892..2389
                     /locus_tag="OO161_002600"
     CDS             1892..2389
                     /locus_tag="OO161_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5463624.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTILR"
     gene            complement(2397..3581)
                     /gene="emrD"
                     /locus_tag="OO161_002601"
     CDS             complement(2397..3581)
                     /gene="emrD"
                     /locus_tag="OO161_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5463625.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLCHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(4000..4089)
                     /gene="tisB"
                     /locus_tag="OO161_002602"
     CDS             complement(4000..4089)
                     /gene="tisB"
                     /locus_tag="OO161_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5463626.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            4654..4752
                     /gene="ivbL"
                     /locus_tag="OO161_002603"
     CDS             4654..4752
                     /gene="ivbL"
                     /locus_tag="OO161_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5463627.1"
                     /translation="MTTSMFNAKLLPTALSVAVVVVRVVVVVGNAP"
     gene            4858..6546
                     /gene="ilvB"
                     /locus_tag="OO161_002604"
     CDS             4858..6546
                     /gene="ilvB"
                     /locus_tag="OO161_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5463628.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            6550..6840
                     /gene="ilvN"
                     /locus_tag="OO161_002605"
     CDS             6550..6840
                     /gene="ilvN"
                     /locus_tag="OO161_002605"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5463629.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            6913..7503
                     /gene="uhpA"
                     /locus_tag="OO161_002606"
     CDS             6913..7503
                     /gene="uhpA"
                     /locus_tag="OO161_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5463630.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            7503..9005
                     /gene="uhpB"
                     /locus_tag="OO161_002607"
     CDS             7503..9005
                     /gene="uhpB"
                     /locus_tag="OO161_002607"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5463631.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGHG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            9015..10334
                     /gene="uhpC"
                     /locus_tag="OO161_002608"
     CDS             9015..10334
                     /gene="uhpC"
                     /locus_tag="OO161_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5463632.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            10472..11863
                     /gene="uhpT"
                     /locus_tag="OO161_002609"
     CDS             10472..11863
                     /gene="uhpT"
                     /locus_tag="OO161_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5463633.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLCFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(11908..>13620)
                     /gene="adeD"
                     /locus_tag="OO161_002610"
     CDS             complement(11908..>13620)
                     /gene="adeD"
                     /locus_tag="OO161_002610"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5463634.1"
                     /translation="ELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIAGV
                     GAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPH
                     EIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHP
                     QVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIENCHE
                     SYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHE
                     GHIDALIRRLIEQYNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKV
                     TVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRYRV
                     IDVIHNELITHSHSSVYSENGFDRDDVYFIAVLERYGQRLAPACGLLGGFGLNEGALA
                     ATVSHDSHNIVVIGRSAEEMALAVYQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDT
                     AQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFTTL
                     EVTE"
BASE COUNT         3068 a         3568 c         3806 g         3178 t
ORIGIN      
        1 cctggctggt tgagtggaaa aatgccactg aaacgcaatg gcttaaagat gccccgtcac
       61 agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc ttccagaatc
      121 gggctgcttt tccccgattc aaaaagcggg gacagaatga tgtattccgc tacccgcagg
      181 gcgttaagct cgatcaggaa aacagccgta tttttctgcc gaaactgggc tggatgcgct
      241 accggaacag ccgttaggtc acgggtgttg tgaaaaatgt cactgtcagc cagtcctgcg
      301 gtaagtggta catcagtatt cagacagaaa gtgaagtatc aactccggtt cacccttcag
      361 catcaatggt cgggctggat gctggcgtgg ctaaactcgc cacgctgtca gatggcacag
      421 tctttgagcc tgtaaacagt tttcagaaaa accagaagaa gctggcgaga cttcagcgac
      481 agttaagccg caaggtcaaa tttagcaaca actggcagaa gcagaaacgc aaaatacgcg
      541 actgcattcc tgtatcgcaa atatccgcag ggactacctt cacaaagtca caacgaccgt
      601 cagcaaaaaa cacgcaatga tagtcattga ggatttgaag gtcagcaaca tgtcaaagtc
      661 agcagcgggt acggtcagcc tgccggggcg caatgtccgg gcaaaatcag gtttaaaccg
      721 ttcgatactg gatcagggct ggtatgaaat gcgccgccag cttgagtaca agcagctctg
      781 gagtggcggt caggtgcttg ctgttccgcc agcgttgcgc gtgctgtggt catacagcga
      841 aagaaaatcg cctgtcacaa agtaaattca gatgccaggt atgtggatat acagcgaacg
      901 ccgatgtaaa tggcgctcgt aacattttag cggcggggca cgccgttctt gcctgtggag
      961 agatggtgca gtcaggccgc ccgttgaagc aggaacccac cgaaatgatt taggcgacag
     1021 cctgaacgta gcagggatcc tcgtccttca gggcgtggag gatgtcaatt tgcgctatga
     1081 tacccatctg acctccttgt agcggatggc aaaagcatat caggaaacgg gcggacgaaa
     1141 ttcttaaaaa aaaccgctaa agatagagcg ttagtgaaat tgcaaaggag caacaatgaa
     1201 aatttcccgc cttggagaag cgcccgatta ccgcttctcg ctggctaatg agcgtacttt
     1261 tctggcgtgg atccgtaccg cactaggatt tctggcggcg ggggttgggc ttgatcaact
     1321 tgcgccagat ttcgccacgc ccgtcattcg tgaactgctg gcgttgttgt tgtgtctgtt
     1381 ttccggtggg ttagcaatgt atggctacct gcgctggtta cgcaatgaaa aagcgatgcg
     1441 tctgaaagag gacctgccct acaccaacag cttattaatc attagcttaa tattgatggt
     1501 cgtggcggtg atcgttatgg gactggtgtt gtatgccgga tagccgcaaa gccagacgca
     1561 ttgccgaccc aggactgcag ccggagcgca catcactggc gtggtttcgt accatgctgg
     1621 gctacggcgc gttgatggcg ttggctatca aacacaactg gcaccaggcg ggcatgttat
     1681 tctggatttc gattggcatt ctcgccatcg tggcgctgat cctctggcac tacacccgta
     1741 atcgcaattt aatggatgtc acgaatagcg atttttccca atttcacgta gtccgtgaca
     1801 aatttttgat ctccctcgcg gtgttatctc tcgcaatact gtttgctgta acgcatatac
     1861 atcaacttat cgtatttatt gagagagtcg catgacagga agtcaggtca ttgatgctga
     1921 agaggacagg cataaactgg ttgtagagta caaggatgct ctgcaacctg ccgattttta
     1981 tcacaatttc aaacagcggg gcatccgctc cgtacagctc attccctatc ttgaatttga
     2041 tgaccggggc gatctgacgg ccgcttcggt gacagcagag ctgtggggga aatttttaat
     2101 tgccctgttc gagtgttggg tgcgggcaga tatcagtcgt atctcgattg agctttttga
     2161 cgccacctta caaaaatggt gtggcggcga aaacccgcaa ccgcgccgtg actgccaggc
     2221 gtgcgactgg catcgcctgt gcccgcacgc tcggcaggag acgccagaca gcgtgctttg
     2281 tgcgggctat caggcgtttt attcttactc cgcaccgcat atgcgggtga tgcgtgatct
     2341 gataaagcag catcgttctc ccatggagct gatgacgata ctgcggtgac gtgcgcttaa
     2401 acgggctgcc cctgatgcga catccgcgtc gccagcggca gccagcacag cacgatcaac
     2461 aatcccatta aggtcatcaa taaccccagg ctaccctgac cggtttgcgg caacatcgca
     2521 gagagcgacg ccagcacgcc ggaaccaatg ttttgcagac cgccgaccag cgcgccagcc
     2581 gtgcccgcca ggaaggggaa cggctccatc gcgccgctgg tcgccagcgg aaacagcatc
     2641 ccggcaccga aaaagaacag cgcggcggga acgagcagcg tccagacatt catcacgcca
     2701 aaccagtcgg ggatccacat cagcaagcca gccagcaggc agcagataac cgactgccac
     2761 attaacgtgg agaagcgttt attgggacgt ccggcaaacc atgcgccaaa aaacgctgcc
     2821 ggaatcggca gaataaacaa aatactgacc gtcatactgc tcagccctaa caccgcgccc
     2881 atcagcacgc cggagcaggc ttcaaaggcg gcaatcccgg ccagaccgcc aatcagcatc
     2941 agcaaataac agttaaaacc gctgttaccg aaaagcgttt tataactggt aagcaggcgc
     3001 gtgcgcggtg catcgaccgg acgcgtttcc ggcatccagc gggccatact gaaggtcaca
     3061 ccaacacaaa gaaccaacaa aaagagataa caggcgcgcc agttccacat cgtatccagc
     3121 agaccgccga ttagcggtgc gagcaacgga ctgacgagaa tccccatgtt taacaggctg
     3181 ttagcatggc acaactgtgt ccgttcatat aaatctcgcg gcaaagtacg cgccattacg
     3241 ccgccaacgc cggtgcccat cccctgcatc gcgctggcgg caatcaatac cgtcaaactg
     3301 gaggtcgtga ccgcgaccag cgttgccagc ataaaaatgg acattccgac gaggatcacc
     3361 ggtcggcgac ccacacggtc ggaaatcggg ccataaaaca gctgtgagac accgtaagtc
     3421 agcagataag cgcccattac gctctgcacc gccccttcac ggacgttgag atcgcgcgcc
     3481 atatcggcaa tagctggaat ataaatggtt tgcgccatct gaccgacggc cacgagtaat
     3541 accaacatca ataacaaatt gacgtttctt tgccttttca ttatcacgga tgcttttata
     3601 aaaataatta gaataagtca ctacgcattc ccataaatgc gggagaggaa tctatcacat
     3661 tgatgaatgt tagccagata tacgccagga atggggaatt gtttagcggc agaatatgta
     3721 aacaaaagcg gcaataaatg ttgccgggag ggattacgtg cgaactgtag ccctctcccc
     3781 aaaggggcga gggggaaacc accgttccct ttcagaaaat gagctaaaca ttgcacggga
     3841 cggtcccctc gcccctttgg ggagagggct agggtgaggg gcacaggcag cgcaggtgca
     3901 aacattctgc tggtagaaga agcccccaac tccaacccgc acgctaaata taaaagggga
     3961 gcggttaccc gctccccttt ggtgcgactt gaatctgaat tacttcaggt atttcagaac
     4021 agcatcaagc agttgcagtg ctgcaacaat gagtttgagg ataagaatgg cgatatccac
     4081 caggctcata cgcgtctcct gtggttcagg agcactgcca gctgacgtac ctttccgctg
     4141 cctgttgcca gctgttgtgc acgcggtatc tcaaggagaa acgttgtgct tgttgacata
     4201 acacagtgtg ctcacgcggg gttaaggcgc tgacggcacc acccgtttca gccaggactt
     4261 agtgcgccgg gtaacgatgc ggcccccgca taatacattg cgtacagtga tattatagaa
     4321 gtttactgta taaataaaca gtaatatttg gacaaaacgc aaactgtgat cgatactcaa
     4381 ctatgcccta aatacgtcaa aattcgtgcc gaaattgcgc gttctgcgcg gaacacgtat
     4441 actttcagtg ttgacataat acagtgtgct ttgcggttac cagctgcagg cggctgatga
     4501 aacctcgctc cggcggggtt ttttgttacc tgcaattcag tacaaaacgt gatcagcccc
     4561 tcaattttcc cttgctgaaa aattttctat tgtctcccct gtaaagctgt gcttgtataa
     4621 atattgttaa acacaaaacc aacaaggtcc ccaatgacta cttccatgtt caacgcaaaa
     4681 ctactaccaa ctgcgctatc cgtcgcagtg gtcgtcgtgc gtgtggtggt ggtcgtcggc
     4741 aatgcgccgt agggactgga acaacacacg attccaaaac cccgccggcg caaaccgggc
     4801 ggggtttttc gtttaagcac ctcccggaaa gtcggcccag aagaaaagga ctggagcatg
     4861 gcaagttcgg gcacaacatc gacgcgtaag cgctttaccg gcgcagaatt tatcgttcat
     4921 ttcctggaac agcagggcat taagattgtg acgggcattc cgggcggttc tatcctgcct
     4981 gtttacgatg ccttaagcca aagcacgcaa atccgccata ttctggcccg tcatgaacag
     5041 ggcgcgggat ttatcgctca ggggatggcg cgcaccgacg gtaaaccggc ggtctgtatg
     5101 gcctgtagcg gaccgggtgc gactaacctg gtgaccgcca ttgccgatgc gcggctggac
     5161 tccatcccgc tgatttgcat caccggtcag gttcccgcct cgatgatcgg caccgacgcc
     5221 ttccaggaag tggacaccta cggcatctct atccccatca ccaaacacaa ctatctggtc
     5281 agacatatcg aagaactccc gcaggtcatg agcgatgcct tccgcattgc gcaatcaggc
     5341 cgcccaggcc cggtgtggat agacattcct aaggatgtgc aaacggcagt ttttgagatt
     5401 gaaacacagc ccgctatggc agaaaaagcc gccgcccccg cctttagcga agaaagcatt
     5461 cgtgacgcag cggcgatgat taacgctgcc aaacgcccgg tgctttatct gggcggcggt
     5521 gtgatcaatg cgcccgcacg ggtgcgtgaa ctggcggaga aagcgcaact gcctaccacc
     5581 atgactttaa tggcgctggg catgttgcca aaagcgcatc cgttgtcgct gggtatgctg
     5641 gggatgcacg gcgtgcgcag caccaactat attttgcagg aggcggattt gttgatagtg
     5701 ctcggtgcgc gttttgatga ccgggcgatt ggcaaaaccg agcagttctg tccgaatgcc
     5761 aaaattattc atgtcgatat cgatcgcgca gagctgggta aaatcaagca gccgcatgtg
     5821 gcgattcagg cggatgttga tgacgtgctg gcgcaattga tcccgcaggt ggaagcgcaa
     5881 ccgcgtgcag agtggcacca gttggtagcg gatttgcagc gcgaattccc gtgtccaatc
     5941 ccgaaagcgt gcgatccatt aagccattac ggcctgatca acgccgttgc cgcctgtgtc
     6001 gatgacaatg cgattatcac caccgatgtg ggtcagcatc agatgtggac cgcgcaagct
     6061 tatccgctca atcgcccacg ccagtggctg acctccggtg ggctgggcac gatgggtttt
     6121 ggcctgcctg cggcgattgg cgcggcgctg gcgaacccgg atcgcaaagt gttgtgtttc
     6181 tccggcgacg gcagcctgat gatgaatatt caggagatgg cgaccgccag tgaaaatcag
     6241 ctggatgtca aaatcattct gatgaacaac gaagcgctgg ggctggtgca tcagcaacag
     6301 agtctgttct acgagcaagg cgtttttgcc gccacctatc cgggcaaaat caactttatg
     6361 cagattgccg ccggattcgg cctcgaaacc tgtgatttga ataacgaagc cgatccgcag
     6421 gctgcattgc aggaaatcat caatcgccct ggcccggcgc tgatccatgt gcgcattgat
     6481 gccgaagaaa aagtttaccc gatggtgccg ccaggtgcgg cgaatactga aatggtgggg
     6541 gaataagcca tgcaaaacac aactcataac aacgtaattc tggagctcac cgttcgcaac
     6601 catccgggcg taatgaccca cgtttgtggc ctttttgccc gccgcgcttt taacgttgaa
     6661 ggcattcttt gtctgccgat tcaggacagc gacaaaagcc atatctggct actggtcaat
     6721 gacgaccagc gtctggagca gatgataagc caaatcgata agctggaaga tgtcgtgaaa
     6781 gtacagcgta atcagtccga tccgacgatg tttaacaaga tcgcggtgtt ttttcagtaa
     6841 ccgctcaagg cttgaacaac atcgcgctta tcgttaaggt aagcgcgttt ttttacccgc
     6901 caggacaaga ccatgatcac cgttgccctt atagacgatc acctcatcgt ccgctccggc
     6961 tttgcgcagc tgctggggct ggaacctgat ttgcaggtag ttgccgagtt tggttcgggg
     7021 cgcgaggcgc tggcggggct gccggggcgc ggtgtgcagg tgtgtatttg cgatatctcc
     7081 atgcccgata tctccggtct ggagctgcta agccagctgc cgaaaggtat ggcgacgatt
     7141 atgctctccg ttcacgacag tcctgcgctg gttgagcagg cgcttaacgc gggggcacgc
     7201 ggctttcttt ccaaacgctg tagcccggat gaactcattg ctgcggtgca tacggttgcc
     7261 acgggcggct gttatctgac gccggatata gccattaaac tggcatccgg tcgtcaggac
     7321 ccgctaacca aacgtgaacg ccaggtggcg gaaaaactgg cgcaaggaat ggcggtgaaa
     7381 gagattgccg ccgaactggg cttgtcaccg aaaacggtac acgtccatcg cgccaatctg
     7441 atggaaaaac tgggcgtcag taacgacgta gagctggcgc gccgcatgtt tgatggctgg
     7501 tgatgaagac gttgttctcc cgcttaatta ccgttattgc ctgctttttt atcttctctg
     7561 ccgcatggtt ttgcctgtgg agtatcagcc tgcatctggt tgagcgccct gatatggcgg
     7621 tgctgttatt tccgtttggt ctgcgtctgg ggctaatgct gcaatgcccg cgcggatact
     7681 ggccggtgct gctgggcgcg gagtggctgc tgatttactg gctaacgcag gcggtcggtt
     7741 taacccattt tccgttattg atgatcggta gtttactgac gttactgccc gtagcgctga
     7801 tctcgcgcta tcgccatcag cgtgactggc gcaccttgct gttacagggg gcggcgttaa
     7861 cggcggcggc gttgttgcag tcgctgccct ggctttggca cggcaaagag tcgtggaatg
     7921 cgctgttgct gactttaact ggcggcctga cgctggcccc gatatgtctg gtgttctggc
     7981 actatctcgc caataacacc tggctgccgc tcggcccgtc actggtttct cagccaatca
     8041 actggcgcgg gcgacatctg gtctggtact tgctgctgtt tgttatcagt ctctggctcc
     8101 agttgggatt gccggacgaa ctgtcgcgct ttacgccatt ctgtctggcg ctgccgatta
     8161 tcgcgctggc ctggcactat ggttggcaag gggcgctgat tgcgacgttg atgaacgcca
     8221 tcgcgctgat cgccagtcaa acctggcgcg atcatccggt ggatttattg ctctcgctgc
     8281 tggtgcaaag tctgacaggg ttgttgcttg gcgctggcat ccagcggttg cgtgaactta
     8341 accagtcgct gcaaaaggaa ctggcgcgca atcagcatct ggctgaacgg ttgctggaaa
     8401 ccgaagagag cgtgcgccgt gatgtggcgc gtgagctgca tgatgatatc ggtcagacca
     8461 tcactgctat tcgtactcag gcgggcattg ttcagcggct ggcggcagat aacgccagcg
     8521 tgaagcagag cgggcagctc atcgaacaac tatcgctggg cgtttacgac gcggtgcgcc
     8581 gtttgttggg gcggttacgt ccgcgccagt tggatgatct caccctggag caggccatcc
     8641 gctcactgat gcgggaaatg gagctggaag ggcacggtat tgtcagccat ctcgaatggc
     8701 gaatcgatga atcagcgtta agcgaaaacc agcgcgtgac gctgtttcgt gtctgccagg
     8761 aagggctgaa caacattgtg aaacatgctg atgccagcgc ggtcaccctg caaggctggc
     8821 agcaggatga acggttgatg ctggttattg aagacgatgg cagcggtttg ccgccaggtt
     8881 ccgggcaaca aggttttggc ctcaccggaa tgcgcgagcg cgtaacggcg ctgggtggca
     8941 cattacacat ttcctgtctg cacggcacgc gtgtcagcgt ttctctacct caacgctatg
     9001 tctaaggttt gatgatgttg ccgtttctga aagcgcctgc cgatgcgcca ttaatgactg
     9061 ataaatatga aattgatgcc cgctatcgct actggcgtcg gcatattctg ctgaccatct
     9121 ggctgggtta cgcgctgttt tacttcacgc ggaaaagttt taacgccgcc gtaccagaaa
     9181 tccttgctaa cggcgtgctc agccgtagcg atatcggcct gttagcgacc ctgttttaca
     9241 ttacctatgg cgtgtcgaag tttgtctccg gcattgtcag cgatcgctca aatgcccgtt
     9301 attttatggg gatagggctt atcgccacgg gcattatcaa cattctgttt ggcttctcga
     9361 cgtcgctatg ggcgtttgcc gtactctggg tgctgaacgc ctttttccag ggctggggtt
     9421 caccggtgtg tgcgcgtctg ttaacggcct ggtattcacg taccgagcgc ggtggttggt
     9481 gggcattatg gaacacggcg cataacgtcg gcggcgcact cattcccatt gtgatggcag
     9541 cggctgcact acattacggc tggcgtgccg ggatgatgat tgctggttgt atggcgatcg
     9601 tcgtgggaat ttttctctgc tggcggctgc gcgatcgccc gcaggcgtta ggtttaccgg
     9661 cggtcggtga atggcgacac gacgcgctgg agatagctca acaacaagaa ggggcagggc
     9721 tgacgcgtaa agagatcctc accaaatatg tgttgctgaa tccgtatatc tggctgcttt
     9781 cgttttgcta tgtgctggtc tatgtggtcc gggcggcgat caacgactgg ggcaatttgt
     9841 atatgtccga gacgctgggc gtcgatctgg tcacggcgaa tacggcagtg acgatgtttg
     9901 aactgggtgg atttatcggt gcgctggtag cgggttgggg ctcggacaaa ttgtttaacg
     9961 gcaaccgagg gccgatgaat ttgattttcg ccgccggaat tttgctttca gtcggctccc
    10021 tgtggctgat gccatttgcc agctacgtga tgcaggcaac ctgcttcttc accattggtt
    10081 tttttgtctt tggcccacag atgttaatcg gtatggcggc ggcagagtgt tcccacaaag
    10141 aggcggcagg ggcggcgacg gggtttgtcg gcttgtttgc ttatctgggg gcgtcgcttg
    10201 ctggttggcc gctggcgaaa gtactcgata cctggcactg gagcggattt tttgtggtta
    10261 tctctatcgc cgccgggatt tccgcactgc tgttactgcc ctttttgaac gcccagacac
    10321 cgcgcgaagc gtgatgcatc tcaccttttc acttcatatc cggcaaaact aagaaatttt
    10381 ccaggttttg cctggacgct atctcaggcc tgatttgctg ctgattttta caatgcatgc
    10441 ctcacgcagg tattcatttc aggagtaacc catgctggct ttcctaaacc aggttcgcaa
    10501 gccgaccctg gaccttccgc tcgaagtgcg gcgcaaaatg tggttcaaac cgttcatgca
    10561 atcctacctg gtggtcttta tcggctacct gacgatgtac ctgattcgca agaactttaa
    10621 catcgcgcag aacgatatga tttcgaccta cgggttgagc atgacgcagc tggggatgat
    10681 cggcctgggt ttctccatca cttatggcgt gggtaaaacg ttggtttcat actacgccga
    10741 cggcaaaaac accaaacaat tcctgccgtt catgctgatc ctgtctgcca tttgtatgct
    10801 gtgcttcagt gccagtatgg gcagcggctc ggttagcctg ttcctgatga ttgccttcta
    10861 cgccttaagc ggctttttcc agagtaccgg cggttcgtgc agttactcca ccatcaccaa
    10921 atggacgccg cgtcgtaaac gcgggacatt cctcggtttc tggaatattt ctcacaacct
    10981 tggcggtgca ggcgcagcag gtgtggcgct gttcggggca aattacctgt tcgatggcca
    11041 tgtcatcggc atgtttatct tcccgtcgat tatcgcgctg attgtcggtt ttatcggcct
    11101 gcgttacggc agcgactccc cagaatctta tggcctcggc aaagctgaag aactgttcgg
    11161 cgaggagatc agcgaagagg acaaagagac agaatctacc gatatgacca agtggcagat
    11221 ctttgttgag tatgtgctga aaaacaaagt gatctggctg ctgtgcttcg ccaacatttt
    11281 cctctatgtg gtacgtattg gtatcgacca gtggtcaacc gtatacgcgt tccaggaact
    11341 gaaactctct aaagcggtgg cgattcaggg ctttacgctg tttgaagctg gtgcgctggt
    11401 cggtacgctg ctgtggggct ggctctctga cctggcgaac ggtcgccgtg gcctggtggc
    11461 ctgcatcgcg ctggcgctga ttatcgccac gctcggtgtg tatcaacatg ccagcaacga
    11521 atatatctat ctggcttctc tctttgcgtt gggtttcctg gtctttggcc cgcaattgtt
    11581 gattggtgtg gctgctgttg gctttgtacc taaaaaagcg attggcgctg ccgatggtat
    11641 taaaggcacc tttgcttacc tgattggtga cagctttgcc aagttaggtc tgggaatgat
    11701 tgccgatggg acgccggtat tcggccttac cggctgggca ggcaccttcg ccgcacttga
    11761 tatcgctgcg attggttgta tctgcctgat ggcgatagtg gcggtgatgg aagaacgcaa
    11821 aatccgccgc gagaaaaaaa ttcagcagtt gacagtggca taaacgtaac tggtgacttt
    11881 tgcccggcat gacgccgggc ttttttatta ttccgtgact tccagcgtag tgaaggcaaa
    11941 cttctcgcca tcaaatagcc cctgactggt tagttttagc gcggggatca ctggcagaga
    12001 aagaaacgcc atctgaataa acggctcatc gggtaacgga ccgcattcac gggcggcggc
    12061 tttcaaggcg tcaatttgtt ccgccagcga ctgcgccgtg tcggtgctca tcaggccggc
    12121 aatgggcaac ggcagatgac tttggacctg gccgttacgt accacgcaca gcccgccgcc
    12181 atcctgaatc acctgataga ccgccagcgc catctcttcg gcactgcgac cgatcaccac
    12241 aatattatgg ctgtcatggc tgaccgtcgc agccagcgca ccttcattca gtccaaagcc
    12301 gccaagcaaa ccacaagccg gagccagccg ttgcccgtaa cgctcaagta cggcaataaa
    12361 gtacacatca tcgcgatcaa aaccattttc gctgtagacg ctggagtggg agtgcgtaat
    12421 caattcgtta tggatgacgt caatgacccg atagcgtttt ccgggcgtaa attgcagggc
    12481 aaagtcgctg gcggaaactg gctggcgggc aatggtgttg ccatatggcg gagcggattg
    12541 tgccagtctc gccgactctt ccgcctgtaa ggtttgcgca tcaatcggct cgcctttcac
    12601 cagtacctgc tgcaccgtga ccttacgcgc atcgctcaac aggacgatat cggcctgctt
    12661 gccgggtgcc agtaagccga ggtgattcag accaaagtgg cgcgccgtcg accagctggc
    12721 gacgcgatat gccacatgca gcggcacatt gtattgttcg atcaggcggc gaattaaggc
    12781 atcgatgtgt ccttcatggg cgatctccca cgggttacgg tcatcggtac agagcatgca
    12841 ttgcgggctg ttaaattcgt tgatcaacgg tgccagcgcg ttgagattgc gggcagcgga
    12901 cccttcgcgg atcatcaacg acatgccgag ttgtaatttc cggcgtcctt cttccagctg
    12961 ataactttcg tggcagtttt caatacccgc agtaatatag gcgttaagtt ctttaccacc
    13021 caaacccggg cagtgaccgt ccagcgtcag gtggcgaaat gcatccagtt tatcgagcag
    13081 cgcattctgc ccgctaatta cgccagggta gtccatcatt tctgcaaggc cggtaacctg
    13141 cggatggtcc cgccaggcga gcatctgttc aagggtaaaa ctggcaccgt taacatcgca
    13201 gccttccagg gcgggtacgc aagagctgac ctgtaagtac tggttttgcc ttgcctgttc
    13261 ggcacagcgg gcaaaccagg cgaatccggc ttctcccatc acgttgacga tttcatgagg
    13321 gtcgcaaata acggtcgtca ggccgcgcgg cagggtagcg gtttcaaagg tgaccggcgt
    13381 catcatgctg gattcaatat gcaggtgagc atcaataaac cctggcaccg ccgttgcgcc
    13441 gcgagcatca atccgctgca aagccggagc atcagcgtat tctgcgccaa caccggcaat
    13501 gtaacgtcct ttaatcacaa ttgggccgga aatttctccg ccattgatca ggtcgagaat
    13561 agagacatta tcaataatat aatcggcaac agcgtcgcca cgggaaacgg ctaacaattc
//
LOCUS       DAJTNA010000105        13485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTNA010000105 DAJTNA010000000
VERSION     DAJTNA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13485
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.106"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(104..3325)
                     /gene="carB"
                     /locus_tag="OO161_002611"
     CDS             complement(104..3325)
                     /gene="carB"
                     /locus_tag="OO161_002611"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5463635.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3343..4491)
                     /gene="carA"
                     /locus_tag="OO161_002612"
     CDS             complement(3343..4491)
                     /gene="carA"
                     /locus_tag="OO161_002612"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5463636.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSALGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KHHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4947..5768)
                     /gene="dapB"
                     /locus_tag="OO161_002613"
     CDS             complement(4947..5768)
                     /gene="dapB"
                     /locus_tag="OO161_002613"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5463637.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(5935..6849)
                     /gene="rihC"
                     /locus_tag="OO161_002614"
     CDS             complement(5935..6849)
                     /gene="rihC"
                     /locus_tag="OO161_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5463638.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6915..7865)
                     /gene="ispH"
                     /locus_tag="OO161_002615"
                     /gene_synonym="lytB"
     CDS             complement(6915..7865)
                     /gene="ispH"
                     /locus_tag="OO161_002615"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5463639.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7867..8316)
                     /gene="fkpB"
                     /locus_tag="OO161_002616"
     CDS             complement(7867..8316)
                     /gene="fkpB"
                     /locus_tag="OO161_002616"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5463640.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8385..8879)
                     /gene="lspA"
                     /locus_tag="OO161_002617"
     CDS             complement(8385..8879)
                     /gene="lspA"
                     /locus_tag="OO161_002617"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5463641.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8879..11695)
                     /gene="ileS"
                     /locus_tag="OO161_002618"
     CDS             complement(8879..11695)
                     /gene="ileS"
                     /locus_tag="OO161_002618"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5463642.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11738..12679)
                     /gene="ribF"
                     /locus_tag="OO161_002619"
     CDS             complement(11738..12679)
                     /gene="ribF"
                     /locus_tag="OO161_002619"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5463643.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12687..12905)
                     /locus_tag="OO161_002620"
     CDS             complement(12687..12905)
                     /locus_tag="OO161_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5463644.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            13008..13271
                     /gene="rpsT"
                     /locus_tag="OO161_002621"
     CDS             13008..13271
                     /gene="rpsT"
                     /locus_tag="OO161_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5463645.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3148 a         3808 c         3399 g         3130 t
ORIGIN      
        1 ccaaaagatt tacttaatgg gcaaaaaaaa cggtcctcat cagagaaccg tattacttat
       61 tcgatcaaat tagcggatga aaaatatccg ccatgacacg ctattatttg atctgcgcgt
      121 gcatttcctg caccgaaatt actttttcgg tcgcgtcggc attcagcgcc atcgcggtag
      181 cgaaaccacc gttcagggtg gtgtcataat gcactttata ttgcagcgca ctgcgacgga
      241 tcacgcggga gtcttcaatc gcgcgacggc ctgaagtggt gttgatgatg taggtatatt
      301 cgccattctt gatgcggtcc tgaatgtgcg gacggccttc atgcaccttg ttcaccagac
      361 gcggattaat gcccgcttcg cccagcacaa tcgccgtgcc gtgggtcgca tccagctcga
      421 agccctgttt cagcagtttt gccgccaggt ccaccacgcg ttctttatcg ccttcgcgca
      481 cggaaagcaa cgcacgaccg tgtttcttca tggtggagtt gctgcccagc tgcgctttgg
      541 caaacgcttc agcgaaggtg cggcccacgc ccatcacttc cccggtggag cgcatttctg
      601 gccctaacag cgggtcaaca cccgggaatt tattgaacgg cagcaccact tctttcaccg
      661 agtagtacgg cgggataact tctttggtta cgccctgctc agccagcgat ttgccagcca
      721 tcacacgcgc cgccactttt gccagcggta cgccggtggc tttggagacg aacggaacgg
      781 tacgcgccgc acgcgggtta acttcaatca ggtagacttc gttgtttttc accgcaaact
      841 gcacgttcat cagaccgcgc acctgcaatt cgaaggccag tttctgcacc tgctggcgca
      901 tcacatcctg aatttcctga cttaaggtgt aggctggcag agaacatgcg gagtcaccgg
      961 agtgcacgcc cgcctgctcg atgtgctcca tgatgccgcc aatcagcacc atttcgccgt
     1021 cgcagatggc atccacgtca acttctaccg cgtcatcaag gaagtgatcc agcaacactg
     1081 gcgcatcgtt agacacgctg accgccgtct ggaagtagcg acgcaggtca gcttcgtcat
     1141 agacgatttc catcgcccga ccgccgagaa cgtaagacgg acgtaccacc agcgggtagc
     1201 caatctcttt cgccttctct accgccattt caatagcggt aacggtggcg ttcgccggtt
     1261 gtttcagttt cagacgctca accgcatgct ggaagcgttc acggtcttct gcacggtcga
     1321 tagcatccgg gctggtgccg ataaccggta cgccagcagc ttccagcgcg cgcgccagtt
     1381 tcagcggggt ctgaccgccg tactgaacga taacgccttt cggcttctca atacgcacga
     1441 tttccagcac atcttccaga gttaccggct cgaagtagag gcgatcggaa gtgtcgtagt
     1501 cggtggagac ggtttccggg ttacagttaa ccataatggt ttcgtaaccg tcttcgcgca
     1561 gcgccagcga ggcgtgtaca cagcagtagt cgaattcgat accctgaccg atacggttcg
     1621 ggccgccgcc gagaaccatg attttttcac ggtcggtaga cggattcgct tcgcactctt
     1681 cttcataagt ggagtacatg taagcggtgt cggtggcgaa ctctgccgca caggtatcca
     1741 cgcgcttata aaccgggtgc aggtcatact ggtcgcgcag cttgcggatt tccgcttcgc
     1801 gcacgcccgc cagttttgcc agacgcgcat cggcaaaacc tttgcgtttc agctggcgca
     1861 ggaattcagc gttcaggcca gtgatgccca cttccgctac tttctcttcc agacgcacca
     1921 gttcttcaat ctgtaccagg aaccagcggt caatattggt caggttgaac acgccgtcca
     1981 cagacaggcc cgcacggaag gcatcggcga tgtaccagat acgctctgcg ccagcatctt
     2041 tcagttcgcg acggattttg gttaacgctt ccgggtcatc gaggctcact ttcgggtcga
     2101 atccagtcgc accgacttcc aggccgcgca gcgctttttg cagggattcc tgctgcgtgc
     2161 gaccaatcgc catcacttcg ccaaccgatt tcatctgagt ggtcagacgg tcgttagcac
     2221 cagcgaattt ttcgaagttg aagcgaggaa ttttagtaac cacatagtcg atggacggct
     2281 cgaaggaggc cggagtacgt ccgccagtga tgtcgttcat cagttcgtcg agggtgtaac
     2341 ccaccgccag tttcgccgcc actttagcaa tcgggaaacc ggtcgctttc gacgccagcg
     2401 ccgaagaacg ggacacgcgt gggttcattt cgataacaat caggcgaccg tttttcgggt
     2461 tcaccgcaaa ctggacattg gaaccaccgg tttcaacgcc gatttcacgc agcaccgcca
     2521 tcgaggcgtt acgcatgatt tgatattctt tgtcggtcag cgtttgggct ggcgcgacag
     2581 tgatggagtc accggtatgg atgcccatcg catcaaagtt ttcgatagag cagacgatga
     2641 tgcagttgtc gtttttatca cgcaccactt ccatctcgta ctctttccag ccgatcagcg
     2701 actcatcaat cagcagctct ttggttgggg agagatccag accgcgggcg caaatttctt
     2761 caaactcttc gcggttataa gcgataccgc cgccgctacc gcccatggta aaggatgggc
     2821 gaataatgca cgggaagccc acgtcagcgg caaccgccag cgcttcttcc atcgtgtgtg
     2881 cgataccgga acgcgcggtt tccagaccaa ttttcttcat cgctacgtcg aaacgacggc
     2941 ggtcttctgc tttatcaatc gcatcggcgg tcgcaccaat catagtcacg ccgaactctt
     3001 ccaacacgcc ctgacgctcc agctccagcg cgcagttcag cgccgtctga ccgcccatcg
     3061 ttggcagcac cgcgtccggg cgctcttttt caataatctt gcgtactact tcccagtgaa
     3121 tcggctcgat gtaggtcgca tcggccattt ccgggtcggt catgatggtc gccgggttgg
     3181 agttcaccag aatgacgcgg taaccctctt cgcgcagggc tttacatgct tgcgcgccag
     3241 agtagtcaaa ctcacacgcc tgaccgataa caatcgggcc cgcacccaga atcaggatac
     3301 tttttatatc tgtacgtttt ggcatggctc ttttactcct gattacttag cggttttacg
     3361 gtactgctca attaactcga taaagtggtc gaacaacggc gctgcgtcgt gtggcccagg
     3421 gctggcttcc gggtgaccct ggaagctgaa cgccggttta tcagtgcgat gaatgccctg
     3481 taacgtaccg tcgaacaggg atttatgcgt gacacgcagg tttgcaggta atgttgcttc
     3541 atccaccgca aaaccgtggt tctgggcggt gatcatcacc acgtttttct ccacatcttt
     3601 aaccggatgg ttgccgccgt ggtgaccaaa tttcattttg acagtcttcg caccgctcgc
     3661 cagcgccagc agctgatgac cgaggcagat gccgaatacc ggaatatcgg tttcgaggaa
     3721 tttctggatg gcggtaatgg cgtaatcgca cggggccggg tcgccaggac cgttggagag
     3781 gaagatgccg tctggattca ttttcagcac atcttccgca gaagtttgcg ccggaacgat
     3841 ggtcaggcga cagcctctat ccaccagcat ccgcaggatg ttgcgcttgg caccaaaatc
     3901 ataagccacg acgtggaacg gcagctcgtc ttcttttttc gcttctggca ggccgccggt
     3961 caacgtccag ctcccttgtg tccagctata ggcttctgcg gtggtcactt cttttgccag
     4021 atccatgcca ttcagacctg ggaacgcgcg ggctttttct aacgccaacg ccgcatccgg
     4081 gttatcgcct gcgataatgc agccattctg tgcgcctttc tcgcgcagta aacgcgtcag
     4141 cttacgggta tcgatatcgg caatcgccac gatgttatgg tgcttcaggt aagaagagag
     4201 gtcttcggta ttacggaagt tgctggcaat cagcggcagg tcgcgaatca ccagaccttg
     4261 tgcatgtacc tgagaagatt cttcatcggc gtcattagtg ccgacattgc caatatgggg
     4321 ataagtaaga gtaacgattt gacgagaata ggaaggatca gtgaggattt cttgataacc
     4381 ggtcattgaa gtattgaaaa cgacttcccc aagcgccgaa cctgttgccc ctatggcccg
     4441 accgtgaaac tgggttccgt cttccagaac caatagcgct gacttaatca aaacaccctc
     4501 catagaatat tcactcactt tatttgcata ttaattcaca atgatgacgt aaatcaatgc
     4561 aaatctgctt accgacgaat ttctggcaaa cggcggcatt ctggagatat gggcgctaaa
     4621 agtcaactta atggcgggat tttttatatt attttgtggc tcatgcatgt tttacaggca
     4681 ttaagcgaca aaaagatcat ataacctgtt ctggaaaacg cttgcgcagc aagaagattg
     4741 catgaaataa aagcgagcag ggaaaagtgg accaaatggt caaattttac attaaaacaa
     4801 caaaaacaga agacaataca tgtaattttt ggcacttata tacaatttaa aaacataaca
     4861 ctaaaaataa aagggccaat aattaaagcc ctttataatc aataaattaa atgtattatt
     4921 tttgcaccat aacaaatatt ttgtggttac aaactattga gatcaagcac atctcgcata
     4981 tcaaaaagac cactttcctt accactcaac cacaaagccg atcttaccgc gccgttagca
     5041 aatgtcatgc ggctggacgc cttatgggtg atctccagac gctcgccaat atcggcaaac
     5101 atcgcggtat gttcgccaac gatgtcacct gcacgcacgg tggcaaaacc aatggtgcca
     5161 ggcacacgtt caccggtgtg gctttcacga ctgtagaccg cgcaatcttt cagatcttta
     5221 tcaagggcgt gggcgatcgc ctctcccatt gccagtgcgg tgcctgacgg cgcatcaact
     5281 ttatgtctat gatgtgcttc aataatttcg atatcggtgt agtcacccat cactttggct
     5341 gctttctcca gcagcttaag catgacgtta acgccaacgc taaaattggc ggcaaagaca
     5401 atcgcaatat cggcggcggc gtcacgaatc gcctgtttac cggcttcgtc aaaccccgta
     5461 gtgccgatca ccatcccttt gccatgctgg cgacaaaaag cgagatggtt cagcgtacct
     5521 tccggacggg taaaatcgat aaacacatca aaatcatctt ttaccgcatc gaggctgctt
     5581 tgcacggtaa cgcctgtttt ccctgcacca gccagctcac cggcgtcgct gcccagtaaa
     5641 gaagatcctt cgcgctccag cgccgcgccc agctgcacgc cttctaatgc cagcgccgcc
     5701 tgaatcaact ggcggcccat acgccccccg gctcccgcga tggcaacgcg gatgtttgca
     5761 tcatgcatag ctattctctt ttgttaattt gcatagaccg ttttcagagt aaccagcgca
     5821 tcgctacgcc gccagccaaa aacgctgata attagaaatt aatgataaac agggagggat
     5881 atcagtgaaa ggcatgagtc gatgaatgac tgcatgccga taacatgtga caggttacga
     5941 cgccagagcc agcacctcag ccacccactg ctggaagcct ttcacatcca gatccaatgc
     6001 cacctttaca ttggctggtt tgcccaggca accgtcgata tcaaccaccg tcgtgcctga
     6061 ggtaaattcg ccctgagttt ccactgccac aaaacacggt ttgagagtga acaattccgg
     6121 gcgcaccagc caggcaatag cgcaaaggtc gtgcatacgc agtccacttt gcatactgcc
     6181 gctacggtag tggctaaaca gggcgtggag catttttccg gtgcgattca actccggcaa
     6241 agtagcgaga tagtcagggg ttaatattgc ctgattggtg acatccagac cgcacatgac
     6301 gatttcgatg ccgctacgga agacacaggc agccgcttct ggatcggcag cgatattaaa
     6361 ctcggcgttt ggtgtgcagt tgccgcgacc agcagaaccg cccatgatca ccagacgacg
     6421 aatatacggt ttacattccg ggcattgtga aagtaacagc gcaatattgg ttaacgggcc
     6481 gatggccacc agggtaacgg gctctggtgc acgcatcagg gcatcccgaa tcgccagaaa
     6541 cgctggtatc ccgagcggct ttcggttgtg ctcaacaaag tcgtagccag ccattcccga
     6601 ttcgccgtgc acagatgccg catcacgcgg tgcgcgtacc agcggcacag cggctccctg
     6661 ggcgagcgga atctccgcat tccagaaatg cagcagttgc agggcgttgc gggtggtttt
     6721 ctcaaccgag acattacccg cgacggtggt catcagttgc aggtcgagtt cgggtgcaaa
     6781 aatcgcggcg gcaatggcga cggcatcgtc aatgccgggg tcagtatcga ggaagatagg
     6841 taaacgcatg ttttctccat aaaaaatgcc ggtaacaaga ccggcatttt cgcataactt
     6901 aggctgctaa tgacttaatc gacttcacga atatcgacac gcagctcttt cggcacttcg
     6961 aaaacaatgt tttcttcacg gccttccagc ggaatggctt caccaccgcc cagctgctgc
     7021 aaacgtgcca ccacattctg caccagaata tccggagccg atgcgcccgc agtcacgccg
     7081 acgcatttaa cctctttcac ccactcttcc tggatgtctt tcgcatcgtc aatcaaaaac
     7141 gcgcgtttgc ccatacgctg ggccagctcc gccagacggt tggagttgga ggagtttttc
     7201 gaaccgacca ccaacacaac ttccgcctgt tctgccaggg cgcgtaccgc ttcctgacgg
     7261 ttagtcgtgg cgtagcagat gtcatctttg cgcggaccga caattttcgg gaagcgttta
     7321 cgcagcgcgt cgatcacatc agacgtgtca tccaccgaca gcgtggtctg ggtcataaag
     7381 gagagcttct cttcgttttt gaccgtcagt ttccacacat cgtccggcga ttcgaccaga
     7441 tacattcccc cttccgggtt actgtactgg cccattgtcc cttccacttc cgggtgcccg
     7501 gcgtgaccga tgagaataga ttcttcgcca cggcgactgg cgcgggcgac ttccatatgc
     7561 actttggtca ccagcggaca ggtcgcgtca aataccgtca aatcgcgact ttttgcttcg
     7621 ttacgtaccg cctgagaaac accgtgtgcg gagaaaatca ggatcgcgcc gtccggtact
     7681 tcgctaatct gctcaataaa gatagcccca cgctcacgca agctatcgac cacatagcgg
     7741 ttatgtacca cttcgtgacg gacatatatc ggtgcgccgt aaatggccag cgcgttttca
     7801 acaatgctga tagcgcggtc taccccggca caaaaaccac gcgggttggc caacaggatc
     7861 tgcatgttac gcctccaatg ccggatcgat ttccagcact tcaatatcaa aatgaacggt
     7921 ctgcccggcc agcggatggt tgaaatcaac ggtaatggag tcgccgttaa tttcgcggat
     7981 cacgccaggc atctcactgc catccattgc ggtaaaaagc atgattgcgc caatttctgg
     8041 ctcgcctgca tccataaatt cacggcggga gaagtactga atcaggtccg gtgacggcac
     8101 gccaaacgcc gcatctggct ccaacgagaa ggtggtttta tcgcccactt tcagccccaa
     8161 caggtgttgc tccagccctt cagaaagaga agcatcaccc aggcggaaca gcgccggttt
     8221 accgttgttg cgggtagact cggcggtggt gccatcgtcg agttttagcg tgaagtgcac
     8281 caggacggcg ctattgctct gtacagattc agacatgcag gtttactctt atatttgagg
     8341 atttttgccg gatgcggcgt aaacgcctta tccggcaggg tttattattg ttttttcgct
     8401 ctagaaggca aaaaaccttc cagcacaatc agtgccgcac cgacacagat ggcagtatcg
     8461 gcaaggttga aggtggcgaa gtgccagtcg ccgacgtaga agtcgatcat atcgacaaca
     8521 aagccgtgcc acaggcggtc gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa
     8581 gcgatattgt ttagcttctg cgtggccttc gagcgataca tcatcactgc caggatcacg
     8641 ctaataccaa tcgcaatacc ggcaaagaac caacgctgcc agccgccgct atcggcaagg
     8701 aaactaaacg ccgcgccata gttacgcgca taatgcagat taagcgacgg gaacagcggg
     8761 accgtatccc ccagagcaaa gttctggagg atcaggtatt tgctgcccag atcgataatc
     8821 agcacgacta ccaccagcca cagccagcgt agccctgttg aacagatcga ttgactcatc
     8881 aggcaaactt acgtttttca ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt
     8941 ctgcgtgttc cgccaccttg ccgacatcct gggtgtagtg ccagcagcgt gggcacttct
     9001 caccttcggc tttactcaac gcgactttca gccctttgag tacttcgctc tgctgagcat
     9061 cagcaggtgc gtcgtgatag tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta
     9121 attcatcgcc cagcgcggtc agtttcgccg ccagttccgg ttctgcatac aaggttactg
     9181 ccgcttccag cgagccaccc actttcttgt cggcacgcgc ttgctcaatg actttgttca
     9241 cttcgccacg cactttcaac agctcgtccc agaacgcatc gttcatcgct tcgctgtctg
     9301 ccaggccaaa caggccttcg taccactcac cggtgaagac gtatttttca cgttcgcccg
     9361 gcaggtagcc ccacacttca tcagcggtga aggagaggat tggtgccatc cagcgcacca
     9421 gcgcttctgc gatgtgatac agcgcagtct ggcagctacg acgcgccaca ctgtccgctt
     9481 tggcggtgta ctgacggtct ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc
     9541 agaagcgcat cagacgctgt accacttcgt ggaaatcgta tgcttcgtac gccttgagga
     9601 tgtcttcctg tgccgctttc gcacaaccta cggcccagcg atccagtacc accatctctt
     9661 ccggtttcac catatctttt gctggatcaa aaccgttcag gtttgccagc aggaagcgcg
     9721 cggtgttacg gatacgacga tagctatcgg cagcacgttt caggatctcg tcagaaacgg
     9781 ccatttcacc ggtgtagtcg gttgatgcca cccacagacg cagaatatcc gcgcccagtt
     9841 tgttcatcac atcctgcggc gaaacggtat tgccgatgga tttagacatc ttgcggccct
     9901 gaccatccac ggtaaagccg tgggtcagta cctgacgata cggcgctttg cctttcatcg
     9961 cggtggagat cattagggaa gacatgaacc aaccacggtg ctggtcagaa ccttccagat
    10021 acatgtccgc tgcgtgaccg gcaaattccg gacgcacgtc aacaacagaa gagtgggtag
    10081 atccggagtc aaaccataca tccaatgtgt ccggcacttt cacgtactga tcagcttcat
    10141 cgccgaggat ctctttcgca tcaagatccc accacgcctg gatgccgtca acttcaacgc
    10201 gttttgccac ttcttccatt agttcgagag tacgcggatg cagctcttcc gtgtctttgt
    10261 gcacgaacag tgacatcggt acgccccagg tgcgctgacg ggagatacac cagtcaggac
    10321 ggttagcaac catcgactcg atacgcgcct ggccccagtc cgggatccac tgcacgcctt
    10381 tgatctcttt cagtgactgc gcacgcagac ctttctgatc catgctgacg aaccactgcg
    10441 gcgtcgcgcg gaagatgatc ggcgttttgt gacgccagca gcacggatag ctgtgctgca
    10501 ttttctcaac gtgcagcagc gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg
    10561 ctttgaagac gttcacgcca tccagcgtcg gataagtgcc cggcagataa gtgccgtccg
    10621 ggccaaccgg gttagcggtt tccaggccgt atttctgacc gatcacatag tcgtccgggc
    10681 cgtggccagg cgcggtgtga acggcaccgg taccggcgtc cagggtaacg tgatcgccga
    10741 ggattgccgg aacgtcgaag cccataaacg gatgggtaaa gcgcagcaac tcaagctccg
    10801 caccttttac cgtgccgaga atggtgtaat cggtcacgcc gatacgctgc attacgcttt
    10861 caaccagatc tttcgccaga atcacggcct gaccgtcgat ctgcaccagc gcatagtcga
    10921 aatcaggtgc aatagagatt gcgcggttcg caggcagagt ccacggcgta gtggtccaga
    10981 tcaccagcga gattgggccg ttaacgttgc ttacggcaaa ttttgctttc agtgcatcct
    11041 gatcgaccgc ctgaaaagca acgtcgatgg acggagaagt tttgtcgtaa tactcaactt
    11101 ccgcttccgc cagcgcagaa cggcagtcaa cgcaccagtg taccggcttc gcgcctttgt
    11161 gcaggtgacc gttgccgatg attttaccca gcgcgcggat gatgttggct tcagttttga
    11221 agtccatggt caggtacggg tgcgaccagt cgcccagcac gcccagacgg ataaagtctt
    11281 tgcgttgacc gtcaacctgg gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg
    11341 cggtgaattt ctcacccggc ttaccgtatt cttgctctac tttcagctcg atcggcagac
    11401 cgtggcagtc ccagccaggc acatacggcg agtcataacc ggaaagccct ttggacttca
    11461 cgataatgtc tttcagaatc ttgttaaccg agtgaccaat atgaatgctg ccattcgcat
    11521 aaggagggcc atcatgcaga atgaaggttt ttttgccttt tttagccgca cggatgatgc
    11581 cgtacagatc atcatcagtc caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc
    11641 cacgcatcgg gaaccctgtt tccggcaaat tcagggttga tttatagtca ctcatcagat
    11701 tctcggttcc gtgtttcggt ttgattacat aacaggctta agccggtttt gttagcccaa
    11761 aaaattcgcg ggcggttaat tcatcacgcg caatctgcgc tttcagttcg tccagcgacg
    11821 caaatcgctg ctcattgcgt atttttttac gcagcactac ttgtatatgg cgaccgtaaa
    11881 ggtccattgc aacatctaac aaatgcactt ccagctgctg gcgaataccg gcaaccgttg
    11941 ggcgtgttcc gatgtttgcc acgccgggta acggcttttc accgaggccc agcacttcta
    12001 ccgcataaac ccctttcacc ggggaaacct gacggcgtag cggtacattc gccgtcggga
    12061 aacctatagt gcgccctaat tcatcaccgt ggactacacg cccggagatg gcaaacgggt
    12121 gccccagtaa actttctgcc agagccagat tgtcatccgc cagcgcctga cgcacagcgg
    12181 tgctgctaat acgcacgcca ccttcgcaaa aagtttgcgt actagtgata tcgaagccgt
    12241 attccatgcc agctttctgt aataacaaga aatcgccttc acgaccagcg ccaaagcgga
    12301 aatcatcccc tacggcaaga aattttacgc gcaaatgctt caccagaaga tcgctgatga
    12361 aattttgcgc ggttaacgcc gcgaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa
    12421 cgccacactc tgcaaggtaa cgcagttttt cccgcagccg ggtcagtctt gccggggctt
    12481 tatcggtagc aaacagttcc agtggttgag gttcaaaaag catcaccatc accggtaagt
    12541 tgcgcttgcg cccttcttcc tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc
    12601 cgtcgaaatt accaatagtc agcacacacc cttcttgcgg ggcctggctg agattatgta
    12661 tgccgcgtat cagcttcatg tctggctcaa aacagtgaaa atcgtccgag tataccttgt
    12721 acagcggtca aggttaaccg gcgattgagt accgaaatca gaaagaagtg gcttttcatt
    12781 gccatggcgc aaataacggg aagaaagtga ccgcctgctg caatttttat cgcggaaaag
    12841 ctgtattcac accccgcaag ctggtagaat cctgcgccat cactacgtaa cgagtgccgg
    12901 cacattaacg gcgcttattt gcacaaatcc attgacaaaa gaaggctaaa agggcatatt
    12961 cctcggcctt tgaattgtcc atatagaaca catttgggag ttggaccttg gctaatatca
    13021 aatcagctaa gaagcgcgcc attcagtctg aaaaggctcg taagcacaac gcaagccgtc
    13081 gctctatgat gcgtactttc atcaagaaag tatacgcagc tatcgaagct ggcgacaaag
    13141 ctgctgcaca gaaagcattt aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag
    13201 gtctgatcca caaaaacaaa gctgcacgtc ataaggctaa cctgactgca cagatcaaca
    13261 aactggctta atcgccaatt tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt
    13321 ttatgcctgc tgcttttatg atgcgtgaga gaacacacta ccggttgcca gatagtcaaa
    13381 agatgctggc ttttgcagac atgccgttac actttctgaa ctcttcgtgg cttaagaatc
    13441 gtagaaccat cggctgatac attctgaaca agaatatcta ctcaa
//
LOCUS       DAJTNA010000106        13391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTNA010000106 DAJTNA010000000
VERSION     DAJTNA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13391
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.107"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            265..897
                     /gene="crp"
                     /locus_tag="OO161_002622"
     CDS             265..897
                     /gene="crp"
                     /locus_tag="OO161_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5463646.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEDQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            948..3038
                     /locus_tag="OO161_002623"
     CDS             948..3038
                     /locus_tag="OO161_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5463647.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(3105..4325)
                     /gene="argD/dapC"
                     /locus_tag="OO161_002624"
     CDS             complement(3105..4325)
                     /gene="argD/dapC"
                     /locus_tag="OO161_002624"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5463648.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(4411..4974)
                     /gene="pabA"
                     /locus_tag="OO161_002625"
     CDS             complement(4411..4974)
                     /gene="pabA"
                     /locus_tag="OO161_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5463649.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(5006..5608)
                     /locus_tag="OO161_002626"
     CDS             complement(5006..5608)
                     /locus_tag="OO161_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5463650.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(5598..5765)
                     /locus_tag="OO161_002627"
     CDS             complement(5598..5765)
                     /locus_tag="OO161_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5463651.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            complement(5870..6442)
                     /gene="ppiA"
                     /locus_tag="OO161_002628"
                     /gene_synonym="rotA"
     CDS             complement(5870..6442)
                     /gene="ppiA"
                     /locus_tag="OO161_002628"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5463652.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            6713..7894
                     /gene="tsgA"
                     /locus_tag="OO161_002629"
     CDS             6713..7894
                     /gene="tsgA"
                     /locus_tag="OO161_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5463653.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            8156..10699
                     /gene="nirB"
                     /locus_tag="OO161_002630"
     CDS             8156..10699
                     /gene="nirB"
                     /locus_tag="OO161_002630"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5463654.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            10696..11022
                     /gene="nirD"
                     /locus_tag="OO161_002631"
     CDS             10696..11022
                     /gene="nirD"
                     /locus_tag="OO161_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5463655.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            11148..11954
                     /gene="nirC"
                     /locus_tag="OO161_002632"
     CDS             11148..11954
                     /gene="nirC"
                     /locus_tag="OO161_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5463656.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            11973..13346
                     /gene="cobA"
                     /locus_tag="OO161_002633"
     CDS             11973..13346
                     /gene="cobA"
                     /locus_tag="OO161_002633"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5463657.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         3171 a         3522 c         3476 g         3222 t
ORIGIN      
        1 acgcgtacta accaaatcgc gcaacggaag gcgacctggg tcatgctgaa gcgagacacc
       61 aggagacaca aagcgaaagc tatgctaaaa cagtcaggat gctacagtaa tacattgatg
      121 tactgcatgt atgcaaagga cgtcacatta ccgtgcagta cagttgatag ccccttccca
      181 ggtagcggga agcatatttc ggcaatccag agacagcggc gttatctggc tctggagaaa
      241 gcttataaca gaggataacc gcgcatggtg cttggcaaac cgcaaacaga cccgactctc
      301 gaatggttct tgtctcattg ccacattcat aagtacccat ccaagagcac gcttattcac
      361 cagggtgaaa aagcggaaac gctgtactac atcgttaaag gctctgtggc agtgctgatc
      421 aaagacgaag agggtaaaga aatgatcctc tcctatctga atcagggtga ttttattggc
      481 gaactgggcc tgtttgaaga ggaccaggaa cgtagcgcat gggtacgtgc gaaaaccgcc
      541 tgtgaagtgg ctgaaatttc gtacaaaaaa tttcgccaat tgattcaggt aaacccggac
      601 attctgatgc gtctgtctgc acagatggcg cgtcgtctgc aagtcacttc agagaaagtg
      661 ggcaacctgg cgttcctcga cgtgacgggc cgcattgcac agactctgct gaatctggca
      721 aaacaaccag acgctatgac tcacccggac ggtatgcaaa tcaaaattac ccgtcaggaa
      781 atcggtcaga ttgtcggctg ttctcgtgaa accgtgggac gcattctgaa gatgcttgaa
      841 gatcagaacc tgatctccgc acacggtaaa accatcgtcg tttacggcac tcgttaatcc
      901 cgtcggagtg gcgcgttacc tggtagcgcg ccattttgtt tcccccgatg tggcgcagac
      961 tgatttatca ccccgatatc aactatgcac ttcgacaaac gctggtgcta tgtttgcccg
     1021 tggccgttgg gttaatgctt ggcgaattac gattcggtct gctcttctct ctcgttcctg
     1081 cctgttgcaa tattgcgggc cttgatacgc ctcataaacg ttttttcaaa cgcttaatca
     1141 ttggtgcgtc gctgtttgcc acctgtagct tactgacaca gctgctactg gcaaaagatg
     1201 ttcccctgcc ctttttgctg accggattaa cgctggtact tggcgtcact gctgagttgg
     1261 ggccattgca cgcaaaattg cttcccgcat cgctgctggc agccattttt accctcagtc
     1321 tggcgggata catgccggtc tgggaaccgt tgctcatcta tgcgttgggc actctctggt
     1381 acggattgtt taactggttt tggttctgga tctggcgcga acaaccgctg cgcgagtcac
     1441 taagtctgct gtaccgtgaa ctggcagatt attgtgaagc caaatacagc ctgcttaccc
     1501 agcacaccga ccctgaaaaa gcgctgccgc cgctgctggt gcgccagcaa aaagcggtcg
     1561 atctaattac ccagtgctat cagcaaatgc atatgctttc cgcgcaaaat aatactgact
     1621 acaagcggat gctgcgtatt ttccaggagg cgctggattt acaggaacat atttcggtca
     1681 gtttgcatca gccggaagag gtgcaaaagc tggtcgagcg tagccatgcg gaagaagtta
     1741 tccgctggaa tgcgcaaacc gtcgccgctc gcctgcgcgt gctggctgat gacattcttt
     1801 accatcgcct gccaacgcgt tttacgatgg aaaagcaaat tggcgcactg gaaaaaatcg
     1861 cccgccagca tccggataat ccggttgggc aattctgcta ctggcatttc agccgcatcg
     1921 cccgcgtgct gcgcacccaa aaaccgctct atgcccgtga cttactggcc gataaacagc
     1981 ggcgaatgcc attacttccg gcgctgaaaa gttatctgtc actaaagtct ccggcgctac
     2041 gcaatgccgg acgactcagt gtgatgttaa gcgttgccag cctgatgggc accgcgctgc
     2101 atctgccgaa gtcgtactgg atcctgatga cggtattgct ggtgacacaa aatggctatg
     2161 gcgcaacccg tctgaggatt gtgaatcgct ccgtgggaac cgtggtcggg ttaatcattg
     2221 cgggcgtggc gctgcacttt aaaattcccg aaggttacac cctgacgttg atgctgatta
     2281 ccaccctcgc cagctacctg atattgcgca aaaactacgg ctgggcgacg gtcggtttta
     2341 ctattaccgc agtgtatacc ctgcaactat tgtggttgaa cggcgagcaa tacatccttc
     2401 cgcgtcttat cgataccatt attggttgtt taattgcttt cggcggtact gtctggctgt
     2461 ggccgcagtg gcagagcggg ttattgcgta aaaacgccca tgacgcttta gaagcctatc
     2521 aggaagcgat tcgcttgatt cttagcgagg atccgcaacc tacgccactg gcctggcagc
     2581 gaatgcgggt aaatcaggca cataacactc tgtataactc attgaatcag gcgatgcagg
     2641 aaccggcgtt taacagccat tatctggcag atatgaaact gtgggtaacg cacagccagt
     2701 ttattgttga gcatattaat gccatgacca cgctggcgcg ggaacaccgg gcattgccac
     2761 ctgaactggc acaagagtat ttacagtctt gtgaaatcgc cattcagcgt tgtcagcagc
     2821 gactggagta tgacgaaccg ggtagttctg gcgatgccaa tatcatggat gcgccggaga
     2881 tgcagccgca cgaaggcgcg gcaggtacgc tggagcagca tttacagcgg gttattggtc
     2941 atctgaacac catgcacacc atttcgtcga tggcatggcg tcagcgaccg catcacggga
     3001 tttggctgag tcgcaagttg cgggattcga aggcgtaata caggctaaac catcggataa
     3061 ggcattcacg ccgcatccga catattttgc ctgatgcgtc ttatttacgc cccaaccacc
     3121 ttcgccaccg cgtgggcgaa acgttgcatc ccttcatcga tatccgcatc ttccaccacc
     3181 agcgacggtg ctaaacgcat cacatccggt ccggcattca gcaccattac gccaacctct
     3241 gcacccgcat acaggaaatc acgcgcccga cctttgtagt gtggtttcag ctccgcgcca
     3301 attaacagcc ccataccgcg aatatcgcta aacacatcgt actgctgatc gatcttctgc
     3361 aaatggtcaa caaaacgctg gcgtttcgcc tgaatgcctt ccagcacttc aggggtattg
     3421 atgatatcaa acgccgcgcc cgctactgca caggccagag gattaccgcc gtaggtggaa
     3481 ccgtgagaac caggatgaaa cgcagaagca atttccgccg tggtcagcat ggcgctaatc
     3541 gggaagccgc cgcctaacgc tttcgcagaa gtcagaatat ccggcgtcac accgtagtac
     3601 atgtaagcaa acaaatcgcc agtccgcccc atcccgcact gcacttcatc aaacaccaat
     3661 aatgcctgat gttgatcgca cagctcgcgc aagccctgca aaaactctgg cgtcgatgcc
     3721 gtcacaccgc cctcgccctg gatcggctca accaccaccg cacaggtgtg atcatccatc
     3781 accgctttca ctgcatggag atcgttaaag ggaacgtgga tgatgtctgc cggtttcggc
     3841 ccaaagccgt cggaatattt tggctgccca cccaccgaaa cggtaaacag cgagcgacca
     3901 tgaaaagcgt tatggaaggc aataattttg gttttgaacg ggctatgacg cacacaggcg
     3961 taatggcgtg ccagtttaaa ggcggtttcg ttagcttccg tgccggagtt cataaacacc
     4021 acgcgttcgg caaacgtcgc ctcaatcagt ttgcgcccaa gacgcagcgc cggttcattg
     4081 gtgaaaacgt tactgatatg ccacagagtt tcgccctggg tttttaacgc gttcaccagc
     4141 gcaggatggc aatggcccaa cgccgtaact gcaatgccac ccgcgaaatc gacatactcc
     4201 ttgccttgct gatcccagat tcggctgccc tgacctttta ccggaataaa ctctgccgga
     4261 gcataaatcg gcaggatcac ttcatcgaaa gtcgcgcgtg taattgctgt ttgttcaatt
     4321 gccatctcat gatcaccctg ttacgcataa ataaatgtga aattacaatc acaaaatatg
     4381 cataaaaaat cactgaatgg caatcggaaa tcagagatgc aggaaattag ccagcagttg
     4441 atgtccttgt tcgctaagaa tactttctgg atggaactgc acaccttcca gatcccactg
     4501 gcgatggcga atccccataa tctcgcgggt ttcgctccag gccgtcacgt caaagcacgc
     4561 tggtaatgag ccaggttcca ccaccagcga atggtagcgt gtcacggtaa gtggatttgc
     4621 cagcccccga aatacgccct caccgttatg tgtaatcggc gaggttttgc cgtgcatgac
     4681 ctttgcggcg cgcacaactt taccgccaaa tgcctgcgcc attgcctgat gaccgaggca
     4741 gacgccaaga atcggcaagc gcccggcata gtggcgaata acgtcaaggg agatcccggc
     4801 ttcatctggc gtacaggggc cgggtgagat gacaattttt tgtggtttaa gggcgtcgat
     4861 atccgccaac gtcaacgcat cgttgcgctt aaccagcaca tccgccccca gttcacaaaa
     4921 gtactggtag aggttccagg taaaagaatc gtagttatca ataagcagga tcatggcggc
     4981 tccggtacga aagaaccgcg ctattttact cagattcccc ggcttcgctt accactttgc
     5041 taaatatcat ctgcagtgcg gtgagatccc ccattgcccc actctgattt gcctgattcc
     5101 aggcctcttt ttcgataccc tgccagctca gttgatatcc ggcatgaatc gccagttgct
     5161 cgaagaagat ccgctgtgcc agaccacttc ccacccggaa gggatgcagc acgttgattt
     5221 cacaatagta atgcgccagc cgctcgacga acttcgtttt ctccaggcca accagataac
     5281 cttcttcctc cagatcctgc atcagggcat tgccctcttt ttctatataa gcaaagtggc
     5341 agaacggcgt atcgccctga taaatatcaa cttcacgcag ttgccctgcc cagtcgaaaa
     5401 tatcctgata cagctggcga tggatagttc gcaaatgcgg taaaccgcgc accagcggac
     5461 caagctcaat ggtcgcagca cgcagcgccg tcatttcgta agcggcctgt tccagccgtt
     5521 gttgctggcg gatgttcagc cggttacgca tgatatcaag gcctggataa agatacggat
     5581 cgcgcccttc gccgaattta tcgctcatag tgcctcctca gctctacaag gcgcgctaaa
     5641 gcctctgcgg cagtaagagt gactaaaggc atctcgacgc cttcaagacg gcgactggcc
     5701 tggaaattac gattacgctg aagctcccag agacgggact tttgcttatc ggtgagtttc
     5761 ttcacttgat gcctccctga acgtgtccat ttgccacaag tataagcggc aaatccgggt
     5821 tacgccggga gagcagaagc gcgggcaaga ttgcccgcgc gagaaatcat tacggcagga
     5881 ctttagcgga aaggataact accggttttg acggcacatt ctggtacgga ccaacgtcat
     5941 gagtcggcac ctgggaaatc ttatcggcca cgtccatgcc tttcaccact ttaccaaata
     6001 ccgcgtaacc gaaatcacgc tgaccatggt caaggaaggc gttatcggca acgttgataa
     6061 agaactggct ggtggcgctg tctttgtcag cggtacgcgc catcgcgatg gtgccacgcg
     6121 tgttgcgcag gccgttatcg gcttcatttt tgattggcgg gtttggtttt ttctgctgca
     6181 tctgctcggt gaaaccgccg ccctgaatca taaagccagg aatgacgcgg tgaaaggtag
     6241 tgttgttata aaaaccgctg ttcacataat cgacaaagtt ttgcacagac actggcgctt
     6301 tttgtttatc cagctccagt tcgatgttac cagctgaggt tgtcaacaat acgtgcgggt
     6361 cccctttcgc tgccattgct gcgggagaaa gagcagaaag agcgaaaaca gcagccatcg
     6421 ccgccagggt cgatttgaac atgagatttc cttaacaaac agagcagaaa aaaagcaagt
     6481 gaagagattc taaatagcct tcaagaacaa ggccatccct ttacctaatt ttacgtatct
     6541 gaaatatatg taacctgacg atcacttttt attccgtgat caagatcacc tcttaaaatg
     6601 caatttagca accgattgca ataaaacatt taaacagatc acaaaatcac ctaaaatcac
     6661 ccgctcgcgg catatcccgc tatgccattt tatttttgaa cgcaggccaa tcatgactaa
     6721 cagcaatcgc atcaagctca catggattag ctttctctcc tacgcactga ctggtgcgtt
     6781 ggttattgtc accgggatgg tgatgggaaa tatcgccgat tatttcaatc tgcctgtttc
     6841 cagtatgagt aacaccttca ccttcctcaa cgccggcatt ttaatctcta tcttcctcaa
     6901 cgcctggctg atggaaatcg tcccgttgaa aacgcagtta cgttttggct ttctcctgat
     6961 ggtgctggcg gttgccggtt tgatgttcag ccacagcctg gcgctgttct cgacggcgat
     7021 gttcattctc ggggtggtca gcggcattac tatgtcgatt ggtacattcc tgataacaca
     7081 aatgtatgaa gggcgtcagc gcggttcccg cctgttattt actgactcct tcttcagtat
     7141 ggctgggatg attttcccaa tgatcgccgc gtttctactg gcgcgcagca ttgagtggta
     7201 ctgggtttat gcctgcatcg ggctggtgta tgtcgctatt tttattctga ccttcggctg
     7261 tgagttccca gcactgggca aacatgcacc aaaaacggat gctccggtag agaaagaaaa
     7321 gtgggggatc ggcgtactgt ttctctctgt tgcggctctg tgctacatcc tcggtcagtt
     7381 aggttttatc tcctgggtgc ctgagtatgc caaaggcctg ggcatgagcc tgaacgacgc
     7441 gggcacgctg gtgagtaact tctggatgtc atacatggtc ggcatgtggg cgttcagctt
     7501 tattcttcgc ttctttgatt tgcaacgcat tctgaccgta ctggctggtc tggctgcgat
     7561 tctgatgtac gtctttaaca ccggaacacc agcacatatg gcgtggtcaa ttctcgctct
     7621 gggcttcttc tccagcgcga tctataccac catcatcacc ctgggttcac agcagaccaa
     7681 agtaccgtcg ccaaaactgg ttaactttgt cctgacctgc ggaaccatcg gtactatgtt
     7741 gacctttgtg gttaccggcc cgattgttga acatagcggt ccgcaggcgg cactgcttac
     7801 agcaaacggt ctgtacgctg tcgtctttgt gatgtgcttc ctgttaggtt tcgtcagccg
     7861 tcaccgtcag cataacaccc tgacctctca ttaattgcta atgccggacg gcactatcgt
     7921 cgtccggcct tttcctctct aaccccgcta cgtgcatcta tttctataaa cccgttcatt
     7981 ttgtctattt tttgcacaaa catgaaatgt cagacaattc cgtgacttaa gaaaatttat
     8041 acaaatcagc aatatacccc ttaaggggta tataaaggtg aatttgattt acatcaataa
     8101 gcggggttgc tgaatcgtta aggtaggcgg taatagaaaa gaaatcgagg caaaaatgag
     8161 caaagtcaga ctcgcaatta tcggtaacgg tatggtcggc catcgcttta tcgaagatct
     8221 tcttgataaa tctgatgcgg ccaactttga tattaccgtt ttctgtgaag aaccgcgcat
     8281 cgcttatgac cgcgtacacc tctcgtctta cttctctcac cacaccgccg aagagctgtc
     8341 gctggtgcgc gaaggcttct acgagaaaca cggcatcaaa gttctggtcg gcgaacgcgc
     8401 tatcaccatc aaccgtcagg agaaggtgat tcactccagc gccggacgta ccgtttttta
     8461 tgacaagctg atcatggcaa ccggttccta cccgtggatc ccaccaatca aaggttctga
     8521 tactcaggac tgctttgtct atcgcaccat tgaagacctc aacgccattg aatcctgcgc
     8581 ccgtcgcagc aaacgcggtg ccgttgttgg tggtggcctg ttaggtctgg aagccgctgg
     8641 cgcgctgaaa aacttaggta ttgaaaccca cgttatcgaa tttgccccta tgctgatggc
     8701 ggaacagctt gatcagatgg gtggcgagca gctgcgtcgc aaaatcgaaa gtatgggcgt
     8761 gcgcgttcac accagcaaaa acacccttga gattgtgcag gaaggtgtcg aagcacgtaa
     8821 aaccatgcgt tttgccgacg gcagcgaact ggaagtcgac tttatcgtct tctctaccgg
     8881 tatccgtccg cgcgataagc tggcaaccca gtgtggtctg gacgttgctc cgcgtggggg
     8941 tatcgtcatt aatgattcct gccaaacttc cgatccggat atctacgcta tcggtgaatg
     9001 cgcaagctgg aacaaccgtg tatttggtct ggtagcacct ggctacaaaa tggcgcaggt
     9061 cgccgttgac catattctcg gtagcgaaaa cgcctttgaa ggtgctgacc ttagcgccaa
     9121 gctgaaactg ctgggcgtgg acgtaggcgg tattggtgat gctcatggtc gcacgcctgg
     9181 cgcacgtagc tacgtttacc tcgacgaaag caaagagatc tacaaacgcc tgatcgtcag
     9241 cgaagacaac aaaaccctgc tcggtgcggt actggtgggc gataccagcg actacggtaa
     9301 cttgctgcaa ctggtgctga atgctatcga gctgccggaa aacccggatt ctctgattct
     9361 gccggcgcac tcgggtagcg gtaagccgtc tatcggtgtt gataaactgc cggacagcgc
     9421 gcaaatctgc tcctgcttcg acgtcactaa aggtgatctg attgctgcca tcaacaaagg
     9481 ctgccacaca gttgcggcgc tgaaagctga aaccaaagcg ggtactggct gcggtggctg
     9541 tatcccgctg gtcactcagg tactgaacgc ggaactggcg aaacagggca tcgaagttaa
     9601 caacaacctg tgcgaacact ttgcttattc gcgtcaggaa ctgttccatt tgatccgcgt
     9661 tgaaggcatt aaaaccttcg aagaactgct ggcgaaacac ggcaaaggct acggttgtga
     9721 agtttgtaaa ccgaccgtcg gttcgctgct ggcctcctgc tggaacgaat acattctgaa
     9781 gccagaacat actccgctgc aagattctaa cgacaacttc ctcgctaaca tccagaaaga
     9841 cgggacctac tcggtgatcc cgcgttctcc gggcggtgaa atcaccccgg aagggctgat
     9901 ggcagtgggt cgtatcgcgc gtgaatttaa tctctacacc aaaatcaccg gttcccagcg
     9961 cctggcgatg tttggcgcac agaaagacga tctgccagag atctggcgtc agctgattga
    10021 agccggcttc gaaaccggtc atgcctatgc gaaagcactg cgtatggcga aaacctgcgt
    10081 gggtagcacc tggtgccgct acggcgttgg cgacagcgtc ggcctcggcg tggaactgga
    10141 aaaccgctac aaaggcatcc gtacgccgca caaaatgaag ttcggtgtct ccggctgtac
    10201 ccgtgaatgt tcagaagctc agggtaaaga cgtgggtatt atcgccactg aaaaaggctg
    10261 gaacctgtat gtttgcggca acggcggcat gaaaccgcgt catgcggatc tgctggcagc
    10321 ggatatcgat cgcgaaacgc tgatcaaata tctcgaccgc ttcatgatgt tctacatccg
    10381 taccgccgac aaactgacgc gtaccgcgcc gtggttagaa aacctcgaag gcggcatcga
    10441 ttacctgaaa gcggtgatca ttgacgacaa actggggctg aacgctcatc tagaagaaga
    10501 gatggcgcgc ctgcgtgaag cggtagtgtg tgagtggact gaaacggtca atacaccgtc
    10561 cgcgcagact cgcttcaaac acttcatcaa cagcgacaag cgtgacccga acgtgcagat
    10621 ggtgccagag cgcgaacagc accgtccggc aacgccgtat gaacgtatcc cggtaactct
    10681 ggtggaggac aacgcatgag ccagtggaaa gacatctgca aaatcgatga catcctgcct
    10741 gaaaccggcg tctgcgcgct gttaggcgac gagcaggtcg cgattttccg cccgtatcac
    10801 agcgaccagg tgtttgcgat cagcaacatc gatccgttct tcgagtccag cgtgctgtca
    10861 cgcgggctga ttgcggaaca ccagggcgag ctgtgggtcg ccagcccgct gaaaaaacag
    10921 cgttttcgct taagtgatgg cttgtgcatg gaagacgaac agttttccgt caaacattac
    10981 gaagcacgag tgaaagacgg cgtggtgcag ctgcgcggtt aatgttttaa cgggaggcgc
    11041 aatgcctccc ctttttgcat ggtcctgtaa tgaccttcgg tatattgcag gacatttttt
    11101 aaactttttg ttttattttt tgtttttatt ttttaaagga taatcaaatg tttacagaca
    11161 ctattaataa gtgtgcggct aacgctgcgc gcattgcacg cctgtcggca aataacccgc
    11221 tcggcttttg ggtcagctcc gccatggcgg gcgcgtatgt gggtcttggg atcatcttga
    11281 ttttcacgct cggtaatttg ctcgacccgt ccgtacgtcc gctggtgatg ggcgcgacct
    11341 ttggtatcgc cttaacgctg gtgattatcg ccggttctga actgttcacc ggacacacca
    11401 tgttcctcac ctttggggta aaagcgggca gcatcagcca cgggcaaatg tgggcaatcc
    11461 tgccgcaaac ctggctgggt aacctggtcg gttccgtctt cgttgccatg ctctatagct
    11521 ggggcggcgg tagcctgctg ccggtagata ccagcatcgt tcactccgtc gcgctggcta
    11581 aaaccactgc accggcaatg gtactcttct tcaaaggtgc attgtgtaac tggctggttt
    11641 gcctggcaat ctggatggcg ctgcgcactg aaggggcggc gaaatttatc gctatctggt
    11701 ggtgtctgct ggcatttatc gcgtccggct acgagcactc tatcgctaac atgacgctgt
    11761 tcgcgctctc ctggttcggc aaccacagcg aagcctacac gctggcgggt attggtcata
    11821 acctgctgtg ggtgacgctg ggtaatactt tatcaggtgc cgtattcatg ggattgggtt
    11881 attggtatgc tacgccgaaa tcgaatcgtc cggttgcgga caaatttaat caaactgaaa
    11941 cggctgccgg ttaattacta aggggttttt acgtggatca tttgcctata ttttgccaat
    12001 tacgcgatcg cgactgtctg attgtcggcg gtggtgatgt cgcggaacgc aaagcaaggt
    12061 tgctgttaga cgcaggcgct cgcttaacgg tgaatgcatt agcgtttatt ccacagttca
    12121 ccgcatgggc agatgcaggc atgttaaccc tcgtcgaagg gccatttgat gaaagccttc
    12181 tcgacacctg ctggttggcg attgcagcga cggatgatga cacgcttaac cagcgcgtca
    12241 gcgaagccgc tgaagctcgt cgcatcttct gtaacgtagt cgatgcgccg aaagccgcca
    12301 gctttattat gccgtcgatt attgaccgct caccgctaat ggtagcggtc tcctctggcg
    12361 gcacctctcc ggttctggcg cgcctgttgc gcgaaaaact cgaatcactg ctgccgttgc
    12421 atctgggcca ggtggcgaaa tatgccgggc aattacgcgg gcgagtgaaa caacagttca
    12481 ccacgatgag tgagcgtcgc cgtttctggg agaaattgtt cgttaacgac cgcctggcgc
    12541 agtcgctggc aaacaacgat cagaaagcca ttactgaaac gaccgaacag ttaatcaacg
    12601 aaccgctcga ccatcgcggt gaagtggtgc tggttggtgc aggtccgggc gatgccgggc
    12661 tgctgacgct gaaaggactg caacaaattc agcaggcaga tgtggtggtc tacgaccgtc
    12721 tggtttctga cgatattatg aatctggtac gccgcgatgc tgatcgcgtt ttcgtcggca
    12781 aacgcgcggg ataccactgt gtaccgcagg aagagattaa ccagatcctg ctgcgggaag
    12841 cgcaaaaagg caaacgcgtg gtgcggctga aaggtggcga tccgtttatt tttggccgtg
    12901 gtggcgaaga gctggaaaca ctgtgcaacg cgggtattcc gttctcggtg gttccgggta
    12961 ttaccgcagc ttctggttgc tctgcctatt cgggtattcc actcacgcat cgcgattatg
    13021 cccagagcgt acgcttaatt accggacact taaaaaccgg tggcgagctg gactgggaaa
    13081 acctggcggc agaaaaacag acgctggtgt tctatatggg gttgaatcag gccgcgacta
    13141 ttcagcaaaa gctgattgaa cacggtatgc ctggcgaaat gccggtggca attgtcgaaa
    13201 acggaacggc agtcacgcag cgcgtgattg acggtacgct cacgcaactc ggtgaacttg
    13261 ctcagcaaat gaacagtcca tcgctaatta ttattggtcg ggttgttggc ctgcgcgata
    13321 aactgaactg gttctccaac cattaattta acccgggcca gagaattctg gccttcttaa
    13381 tgactctttt t
//
LOCUS       DAJTNA010000107        13215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTNA010000107 DAJTNA010000000
VERSION     DAJTNA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13215
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.108"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            7..82
                     /locus_tag="OO161_002634"
     tRNA            7..82
                     /locus_tag="OO161_002634"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Trp,seq:cca)
     gene            complement(178..1017)
                     /gene="hdfR"
                     /locus_tag="OO161_002635"
     CDS             complement(178..1017)
                     /gene="hdfR"
                     /locus_tag="OO161_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5463658.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDNTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            1136..1474
                     /gene="maoP"
                     /locus_tag="OO161_002636"
     CDS             1136..1474
                     /gene="maoP"
                     /locus_tag="OO161_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5463659.1"
                     /translation="MAESFTTTNRYFDNKNYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            complement(1498..1765)
                     /locus_tag="OO161_002637"
                     /pseudo
     CDS             complement(1498..1765)
                     /locus_tag="OO161_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein"
     gene            complement(1762..2922)
                     /locus_tag="OO161_002638"
     CDS             complement(1762..2922)
                     /locus_tag="OO161_002638"
                     /inference="COORDINATES: protein motif:HMM:NF007365.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR5463660.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTKVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYGLVGELALTGALRGVPGAISSTTEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVIGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFHSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCVISTDSLVPFSTASISHWRSHYRLPVF"
     gene            3273..3371
                     /gene="ilvL"
                     /locus_tag="OO161_002639"
     CDS             3273..3371
                     /gene="ilvL"
                     /locus_tag="OO161_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5463661.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            3458..3508
                     /locus_tag="OO161_002640"
     CDS             3458..3508
                     /locus_tag="OO161_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463662.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            3511..5157
                     /locus_tag="OO161_002641"
     CDS             3511..5157
                     /locus_tag="OO161_002641"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5463663.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            5154..5417
                     /gene="ilvM"
                     /locus_tag="OO161_002642"
     CDS             5154..5417
                     /gene="ilvM"
                     /locus_tag="OO161_002642"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5463664.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            5437..6366
                     /gene="ilvE"
                     /locus_tag="OO161_002643"
     CDS             5437..6366
                     /gene="ilvE"
                     /locus_tag="OO161_002643"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5463665.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            6431..8281
                     /gene="ilvD"
                     /locus_tag="OO161_002644"
     CDS             6431..8281
                     /gene="ilvD"
                     /locus_tag="OO161_002644"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5463666.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            8284..9828
                     /gene="ilvA"
                     /locus_tag="OO161_002645"
     CDS             8284..9828
                     /gene="ilvA"
                     /locus_tag="OO161_002645"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5463667.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(9825..10715)
                     /gene="ilvY"
                     /locus_tag="OO161_002646"
     CDS             complement(9825..10715)
                     /gene="ilvY"
                     /locus_tag="OO161_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5463668.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            10865..12340
                     /gene="ilvC"
                     /locus_tag="OO161_002647"
     CDS             10865..12340
                     /gene="ilvC"
                     /locus_tag="OO161_002647"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5463669.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            complement(12427..12708)
                     /gene="ppiC"
                     /locus_tag="OO161_002648"
     CDS             complement(12427..12708)
                     /gene="ppiC"
                     /locus_tag="OO161_002648"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5463670.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(12907..>13215)
                     /locus_tag="OO161_002649"
     CDS             complement(12907..>13215)
                     /locus_tag="OO161_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5463671.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINTISLIN
                     "
BASE COUNT         3158 a         3421 c         3576 g         3060 t
ORIGIN      
        1 ctaattaggg gcgtagttca attggtagag caccggtctc caaaaccggg tgttgggagt
       61 tcgagtctct ccgcccctgc cagaaatcat ccttagcgaa agctaaggat tttttttatc
      121 tgaaataacc ctctccgaag taaatccttc taccggcatc cttgccagcc attcatatta
      181 atacacttca tccagcacgt taattttcaa aagatcgcga atcaacgcat ttttatcgct
      241 attttgcagc catatggcat aaagcggccg tgaaagtgtt gtgttatcga caacggtatg
      301 caggccgcct tttttacgcg cccagctgac gggtagccag gtgcaaccat taagcatcgc
      361 aatctgttgc tgtgccagtt cagcagaact ggttgtcaga atgggcactt cgtcagcacc
      421 gatcaaacct gcctcatgct gttgaaaatc tggcccccac tcaagtcgca gataattaag
      481 atctcccttt agttttgaag gggcactggt ataaagcgct aaagtgaaat atcccagcaa
      541 ctgactacta aattcgtcca ttttgggcgc ttcagtggta ataagaagat caagctggcg
      601 ttcatgcagc tgttttacca gagactgccg ttgggcaatt cgcgcttcga actgtaagcc
      661 tgtatgggca tcctgatttt gatacaaacg tcccagccac tgattaagca tacattccca
      721 caatgaggcg ctggcaccga tagaaaactc gttatgtcgt gaggtatgcg ccacctcctt
      781 gcgggcggcc tgccaggtgc tcatgagcgt ttctgcataa ggcagtagtt tttcaccggc
      841 agcggttaaa cggatattgt ttctgtggcg ggtgaaaagg ttcacaccca gttgattttc
      901 cagttgtctg attcgaaagc tcactgctga ctgggtcaga tagagcgatt cagccgctcg
      961 accaaagtga cgcgttcggc taacttccag gaaagttttt aacaattccg tatccacagt
     1021 gctctccgca aaattatttg ttgttatgat ttaaatgttt tgttttacac tctgtcaagc
     1081 gtaactaata ctccgcgcca taactagctc ggtcaaagaa ttaggagcgt gcaggatggc
     1141 ggaaagcttt acgacgacta atcgatattt cgacaataaa aattatccac gtggattctc
     1201 tcgtcatggt gatttcacca tcaaagaggc acaactgctt gagcgtcatg gttatgcctt
     1261 caatgagttg gatcttggca aacgcgagcc ggttaccgag gaagagaaac tcttcgtagc
     1321 agtatgccgt ggcgaacgtg agccagtgac agaagcagaa cgcgtgtggt ccaagtatat
     1381 gacgcgtatt aagcgtccaa aacgttttca caccctttcc ggcggtaaac cgcaggttga
     1441 aggtgctgaa gactacaccg attctgacga ttaataaaaa gggcgaaatg ccctttttta
     1501 tgtcagtagt ttctgcagat ggatgagcaa acggtcaatc gctcgatagc taactgcctc
     1561 ctgcaaatgt tgacgtgtga taatgtcaga ctgatcaata tcagcaatgg ttcgtgcaac
     1621 tttcaataac cgctgccagg cacgaatcga taaccccaga tggatcagcg tttcttccag
     1681 ccacatcgca tcttcgctct caggcggcca ttacgcgttg tttaacggtg gcgctgcttt
     1741 ctcccggcac taccgtttta ctcaaaatac cgggaggcgg taatgggatc tccagtgaga
     1801 gatcgaagcg gtcgagaaag ggaccagaga gtcggttgag ataacgcagc ggttatggtt
     1861 tccctgataa tgtccggtag ggctgggatt catcgccgcg acaagctgga aacgggctgg
     1921 ataggttatt tttgctcgcg tgcgtgaaag atggatctgc ccggattcaa tcggctctcg
     1981 caaggcatcc agtgtacgcc tttcaaattc aggtaactca tcaagaaaaa gcacgccgtt
     2041 atgcgccagc gaaatttcac cgggccctgg aattgcgcca ccgcctacca tcgcagttaa
     2101 cgatgcactg tgatgaggtg agtggaacgg gcgctgccgc cattgttttt gtactgattc
     2161 agcatttacc agacttaata tcgcagcact ctccagtgcc tcttcattgc ttaaatctgg
     2221 caacaggcca ttaatacggc tggcgagcat tgttttacct gttcccggcg gcccaatcag
     2281 taaaaggttg tgcccgccag cggcggtaat ttccagtcct cgctttcctt gttcctgacc
     2341 gataacatca ctgagatcat gttgtagcgc ccgggatact gcatcagttg gtttcgggcg
     2401 ttcgagagcg tgcttacctt ccagaaacgc acagacagct tgcaggtgat cggctatcag
     2461 gcatccttca ccgttaatta gccccacttc atcttcgtta tctttcgcga cgataatttt
     2521 tctgcccgac ttaatagctt cagttgtact ggagattgcg ccgggaacgc cacgcagagc
     2581 gcctgtaagc gccagttctc cgactaatcc atattcatct aacttattgg ctgtaagctg
     2641 ttctgaggcc gccagcaacg caatggcgat aggtaaatca tatcgtcccc cttcttttgg
     2701 cagatcagct ggagccaggt tgatggtgat ttttttcgcc ggatattcat atccgctatt
     2761 gataatggcg ctgcgcacgc gatcgcgagc ttcttttacc gttgtttctg gtaagcccac
     2821 cttcgttaag ccgggtagac ctttactgat atgtacctca acagtgatcg ggggcgcatt
     2881 tactcccagg gctgcgcggg tatgaacaat tgacagtgac ataagccctc cttgagtcac
     2941 cattatgtgc ataagatatc gctgctgtag cccgctaatt cgtgaatttt agtggctgat
     3001 tcctgtttat ttgtgcaagt gaagttgagt tgttctggcg gtggaatgat gctcgcaaaa
     3061 atgcagcgga caaaggatga actacgagga agggaacaac attcatactg aaattgaatt
     3121 tttttactca ctattttatt tttaaaaaaa acaacttata ttgaaattat taaacgcatc
     3181 ataaaaatcg gccaaaaaat atcttgtact atttacaaaa cctatggtaa ttctttaggc
     3241 attccttcga acaagatgca agaaaagaca aaatgacagc ccttctacga gtgattagcc
     3301 tggtcgtgat tagcgtggtg gtgattatta tcccaccgtg cggggctgca cttggacgag
     3361 gaaaggctta gaaatcaagc cttaacgaac taagaccccc gcaccgaaag gtccgggggt
     3421 tttttttgac cttaaaaaca taaccgagga gcagacaatg aataacagca caaaattctg
     3481 tttctcaaca ttcagcacgg ggaactaact atgaatggcg cacagtgggt ggtacatgcg
     3541 ttgcgggcac agggtgtgaa caccgttttc ggttatccgg gtggcgcaat tatgccggtt
     3601 tacgatgcat tgtatgacgg cggcgtggag cacttgctgt gccgacatga gcagggtgcg
     3661 gcaatggcgg ctatcggtta tgcccgtgct accggcaaaa ctggcgtatg tatcgccacg
     3721 tctggtccgg gcgcaaccaa cctgataacc gggcttgcgg acgcactgtt agattctatc
     3781 cctgttgttg ccatcaccgg tcaagtgtcc gcaccgttta tcggcactga tgcatttcag
     3841 gaagtggatg tcctgggatt gtcgttagcc tgtaccaagc acagctttct ggtgcagtcg
     3901 ctggaagagt tgccgcgcat tatggctgaa gcattcgacg ttgccagctc aggtcgtcct
     3961 ggtccggttc tggtcgatat cccaaaagat atccagctag ccagcggtga cctggaaccg
     4021 tggttcacca ccgttgaaaa cgaagtgact ttcccacatg ccgaagttga gcaagcgcgc
     4081 cagatgctgg caaaagcgca aaaaccgatg ctgtacgttg gtggtggcgt gggtatggcg
     4141 caggcagttc ctgctttacg agaatttctc gctaccacaa aaatgcctgc cacctgcacg
     4201 ctgaaagggc tgggcgcagt tgaagcagat tatccgtact atctgggcat gctgggaatg
     4261 catggcacca aagcggcgaa cttcgcggtg caggagtgcg acttgctgat cgccgtgggt
     4321 gcacgttttg atgaccgggt gaccggcaaa ctgaacacct tcgcaccaca cgccagcgtt
     4381 atccatatgg atatcgaccc ggcagaaatg aacaagctgc gtcaggcaca tgtggcatta
     4441 caaggtgatt taaatgctct gttaccagaa ttacagcagc cgttaaatat caatgactgg
     4501 cagcaatact gcgcgcagct gcgtgatgaa catgcctggc gttacgacca tcccggcgac
     4561 gctatctacg cgccgttgtt gttaaaacaa ctgtcggatc gtaaacctgc ggattgcgtc
     4621 gtgaccacag atgtggggca gcaccagatg tgggctgcgc aacacatcgc ccacactcgc
     4681 ccggaaaatt tcatcacctc cagcggctta ggcactatgg gttttggttt accagcggcg
     4741 gttggcgcac aagtcgcgcg accgaacgat accgtcgtct gtatctccgg tgacggctct
     4801 ttcatgatga atgtgcaaga gctgggcacc gtaaaacgca agcagttacc gttgaaaatc
     4861 gtcttactcg ataaccaacg gttagggatg gttcgacaat ggcagcaact gttttttcag
     4921 gaacgataca gcgaaaccac ccttactgat aaccccgatt tcctcatgtt agccagcgcc
     4981 ttcggcatcc ctggccaaca catcacccgt aaagaccagg ttgaagcggc actcgacact
     5041 atgctgaaca gtgatgggcc atacctgctt catgtctcaa tcgacgaact tgagaacgtc
     5101 tggccgctgg tgccgcctgg cgccagtaat tcagaaatgt tggagaaatt atcatgatgc
     5161 aacatcaggt caatgtatcg gctcgcttca atccggaaac cttagaacgt gttttacgcg
     5221 tggtgcgtca tcgtggtttc cacgtctgct caatgaatat ggccgccgcc agcgatgcac
     5281 aaaatataaa tatcgaattg accgttgcca gcccacggtc ggtcgactta ctgtttagtc
     5341 agttaaataa actggtggac gtcgcacacg ttgccatctg ccagagcaca accacatcac
     5401 aacaaatccg cgcctgagcg caaaaggaat ataaaaatga ccacgaagaa agctgattac
     5461 atttggttca atggggagat ggttcgctgg gaagacgcga aggtgcatgt gatgtcgcac
     5521 gcgctgcact atggcacttc ggtttttgaa ggcatccgtt gctacgactc gcacaaagga
     5581 ccggttgtat tccgccatcg tgagcatatg cagcgtctgc atgactccgc caaaatctat
     5641 cgcttcccgg tttcgcagag cattgatgag ctgatggaag cttgtcgtga cgtgatccgc
     5701 aaaaataatc tcaccagcgc ctatatccgt ccgctgatct tcgtcggtga tgttggcatg
     5761 ggagtaaacc cgccagcggg atactcaacc gacgtgatta tcgctgcttt cccgtgggga
     5821 gcgtatctgg gcgcagaagc gctggagcag gggatcgatg cgatggtttc ctcctggaac
     5881 cgcgcagcac caaacaccat cccgacggcg gcaaaagccg gtggtaacta cctctcttcc
     5941 ctgctggtgg gtagcgaagc gcgccgccac ggttatcagg aaggtatcgc gctggatgtg
     6001 aacggttaca tctctgaagg cgcaggtgaa aacctgtttg aagtgaaaga cggcgtgctg
     6061 ttcaccccac cgtttacctc atccgcgctg ccgggtatta cccgtgatgc catcatcaaa
     6121 ctggcgaaag agctgggaat tgaagtgcgt gagcaggtgc tgtcgcgcga atccctgtac
     6181 ctggcggatg aagtgtttat gtccggtact gcggcagaaa tcacgccagt gcgcagcgta
     6241 gacggtattc aggttggcga aggccgttgt ggcccggtta ccaaacgcat tcagcaagcc
     6301 ttcttcggcc tcttcactgg cgaaaccgaa gataaatggg gctggttaga tcaagttaat
     6361 caataaatac aaaaaatggg acggcacgca ccgtcccatt tacgagacag acactgggag
     6421 taaataaagt atgcctaagt accgttccgc caccaccact catggtcgta atatggcggg
     6481 tgctcgtgcg ctgtggcgcg ccaccggaat gaccgacgcc gattttggca agccgattat
     6541 cgcggttgtg aactcgttca cccaatttgt accgggtcac gtccatctgc gcgatctcgg
     6601 taaactggtc gccgaacaaa ttgaagcggc tggcggcgtt gccaaagagt tcaacaccat
     6661 tgcggtggat gatgggattg ccatgggcca cggggggatg ctttattcac ttccatctcg
     6721 cgaactgatc gctgattccg ttgagtatat ggtcaacgcc cactgcgccg acgccatggt
     6781 ctgcatctct aactgcgaca aaatcacccc ggggatgctg atggcttccc tgcgcctgaa
     6841 tattccggtg atctttgttt ccggcggccc gatggaggcc gggaaaacca aactgtccga
     6901 tcagataatc aaactcgatc tggttgatgc gatgatccag ggcgcagacc cgaaagtttc
     6961 tgactcccag agcgatcagg ttgaacgttc cgcctgccca acctgcggtt cctgctccgg
     7021 gatgtttacc gctaactcaa tgaactgcct gaccgaagcg ctgggtttgt cgcagccagg
     7081 caacggctcg ctgctggcaa cccacgcgga ccgtaagcag ctgttcctta atgctggtaa
     7141 acgcattgtt gaattgacca aacgttatta cgagcaaaac gacgaaagtg cactgccgcg
     7201 taatatcgcc agtaaggcgg cgtttgaaaa cgccatgacg ctggatatcg cgatgggtgg
     7261 atcgactaac accgtacttc acctgctggc ggcggcgcag gaagcggaaa tcgacttcac
     7321 catgagtgat atcgataagc tttcccgcaa ggttccacag ctgtgtaaag ttgcgccgag
     7381 cacccagaaa taccatatgg aagatgttca ccgtgctggt ggtgttatcg gtattctcgg
     7441 cgaactggat cgcgcggggt tactgaaccg tgatgtgaaa aacgtgctcg gcctgacgtt
     7501 gccgcaaacg ctggagcaat acgacgttat gctgacccag gatgacgcgg taaaaaatat
     7561 gttccgcgcc ggccctgcgg gtatccgtac cacccaggca ttctcgcaag attgccgttg
     7621 ggatacgctg gacgacgatc gcgcaaacgg ctgtatccgc tcgctggaac acgcctacag
     7681 caaagacggc ggcctggcgg tgctctacgg taattttgct gaaaacggct gcatcgtgaa
     7741 aaccgcgggc gtcgatgaca gcatcctcaa attcaccggc ccggcaaaag tgtacgaaag
     7801 ccaggacgac gcggtagaag cgatcctcgg tggcaaagtt gtcgcgggcg atgtggtggt
     7861 gatccgctat gaaggcccga aaggcggccc tggcatgcag gaaatgctct acccaaccag
     7921 cttcctgaaa tcaatgggtc tcggtaaagc ctgtgcgctg atcaccgacg gtcgtttctc
     7981 tggcggcacc tctggtcttt ccatcggcca cgtctcaccg gaagcggcaa gcggcggcag
     8041 cattggtctg attgaagatg gtgacctgat cgctatcgac atcccgaacc gtggcattca
     8101 gttacaggta agcgatgccg aactggcggc gcgtcgtgaa gcgcaggaag ctcggggtga
     8161 caaagcttgg acgccgaaaa accgtgaacg tcaggtttcc tttgccctgc gcgcctacgc
     8221 cagcctggca accagcgccg acaaaggtgc ggtgcgcgat aaatcgaaac tggggggtta
     8281 ataatggctg actcgcaacc cctgtccggt gctccggaag gtgctgaata tttaagagca
     8341 gtgctgcgcg cgccggttta cgaggcagcg caggttacgc cgctacaaaa aatggaaaaa
     8401 ctgtcgtcgc gtcttgataa cgtcattctg gtgaagcgcg aagatcgcca gccagtgcac
     8461 agctttaagc tgcgcggcgc atacgccatg atggcgggcc tgacggaaga acaaaaagcg
     8521 cacggcgtga tcactgcttc tgcgggtaac cacgcgcagg gcgtcgcgtt ttcttctgcg
     8581 cggttaggcg tgaaggccct gatcgttatg ccaaccgcca ccgccgacat caaagtcgat
     8641 gcggtacgtg gattcggcgg cgaagtgctg cttcatggcg cgaactttga cgaagcgaaa
     8701 gctaaagcga tcgaactgtc acagcagcag gggttcacct gggtgccgcc gttcgaccat
     8761 ccgatggtga ttgccgggca aggcacgctg gcgctggaac tgctccagca ggacgcccat
     8821 ctcgaccgcg tatttgtgcc agtcggcggc ggcggtctgg cggcgggcgt tgcggtgctg
     8881 atcaaacaac tgatgccaca aatcaaagtg atcgccgtag aagcggaaga ctccgcctgc
     8941 ctgaaagcag cgctggatgc gggtcatccg gttgatctgc cgcgcgtagg gctatttgct
     9001 gaaggcgtag cggtaaaacg catcggtgac gaaaccttcc gtttatgcca ggagtatctc
     9061 gacgacatca tcaccgtcga tagtgatgcg atctgtgcgg cgatgaagga tttattcgaa
     9121 gatgtgcgcg cggtggcgga accctctggc gcgctggcgc tggcgggaat gaaaaaatat
     9181 atcgcccagc acaacattcg cggcgaacgg ctggcgcata ttctttccgg tgccaacgtg
     9241 aacttccacg gcctgcgcta cgtctcggaa cgctgcgaac tgggcgaaca gcgtgaagcg
     9301 ttgttggcgg tgaccattcc ggaagaaaaa ggcagcttcc tcaaattctg ccaactgctt
     9361 ggcgggcgag cggtcaccga gttcaactac cgttttgccg atgccaaaaa cgcctgcatc
     9421 tttgtcggcg tgcgcctgag ccgcggcctc gaagagcgca aagaaatttt gcagatgctc
     9481 aacgacggcg gctacagcgt ggtggatctc tccgacgacg aaatggcgaa gttgcatgtg
     9541 cgctatatgg tcggcgggcg tccgtcacat ccgttgcagg aacgcctcta cagcttcgaa
     9601 ttcccggaat caccgggcgc gctgctgcgc ttcctcaaca cgctgggtac gcactggaac
     9661 atttctttgt tccactatcg cagccatggc accgactacg ggcgcgtact ggcggcgttc
     9721 gaactcggcg accatgaacc ggatttcgaa acccggctaa atgagctggg ctacgattgc
     9781 cacgacgaaa ccaataatcc ggcgttcagg ttctttttgg cgggttaatg gttcggcaaa
     9841 atcttccaga acgcctcaat cagcggctca tgcagccgct ttttctgcgc acaaacgccc
     9901 agttcaaacg gcgttttttc atcgctgcgc tctaaaatca tcacgcggtt acgcaccggt
     9961 tcggggctgt tttccagcac tacttccggc aacagcgcca cgccacagcc gagcgccacc
    10021 atcgatacca tcgcttcatg cccgccaacc gtggcgtaaa tcatcgggtt gctgatttta
    10081 ttgcgtcgaa accacagttc aatgcggcgg cgtaccggtc cctgatcggc catgataaac
    10141 ggcactgtag accagtccgg cttctctgac gaaacctggt tacgcaccgg gcagggcagc
    10201 gcgggcgcaa tcagcactac tgccagattc tccagcatcg aaaacgccac tgcgccgggc
    10261 agagtctccg gtttcccggc aatcgccaga tccgcttcgc cagtgactac cttttccatc
    10321 gcatctgccg catcaccagt ggttagtttg atctccaccg acgggtgttc cgcgcggaag
    10381 cggtcgagaa tcggtggcag atggctgtag gcagcggtca ccgagcagaa gatatgtaat
    10441 tcgccagaga gcgacggccc ttgctgatcg atggtgtggc gcaactgctg atactgcaac
    10501 agcgtttgct gggcgaaaac gcgcagctct tcgcccgctt cagtcagcgt caccgtgcgg
    10561 ttatcgcgca caaatagcgg ctgaccaaga tcttcttcca ggcgctgaat ctgccgtgag
    10621 agcgtggatg ggctaacgtg catcgcccgc gcgctgcggc caaaatggcg gctttccgcc
    10681 agatgcagga aggttttcag atcgcgtaaa tccaatggag cagccctcaa tgttgacgtt
    10741 gcagaaattg caatgtgacg ttgtgaatat atcaatttcc gcaataaatt tcctgtcata
    10801 tagtgaattc aatctcgcaa acgcgaaccg aacaataaga agcacaacat cacgaggaat
    10861 caccatggct aactacttca atacactgaa cctgcgccag cagctggcac agctgggcaa
    10921 atgtcgcttt atggggcgcg acgaattcgc cgacggcgcg agctaccttc agggtaaaaa
    10981 agtggttatc gtcggctgtg gcgcacaggg gctgaaccag ggcctgaaca tgcgtgattc
    11041 tggtctcgat atctcctacg ctctgcgtaa agaagcgatt gccgagaagc gcgcatcctg
    11101 gcgtaaagcg accgaaaacg gttttaaagt gggcacttac gaagaactga tcccgcaggc
    11161 ggatctggtg gttaacctga cgccagacaa gcagcactcc gacgtagtgc gcaccgtaca
    11221 gccactgatg aaagacggcg cggcgctggg ttactcgcac ggtttcaaca tcgtcgaagt
    11281 gggcgagcag atccgtaaag acatcaccgt agtcatggtt gcgccgaaat gccctggcac
    11341 cgaagtgcgt gaagagtaca aacgtggatt cggcgtaccg acgctgattg ccgttcaccc
    11401 ggaaaacgat ccgaaaggcg aaggcatggc gatcgctaaa gcatgggcgg cagcaaccgg
    11461 tggtcaccgt gcgggcgtgc tggaatcgtc cttcgttgcg gaagtgaaat ctgacctgat
    11521 gggtgagcaa accatcctgt gcggtatgtt gcaggctggc tcgctgctgt gcttcgacaa
    11581 gctggtggaa gagggcaccg atccggcata cgcagaaaaa ctaattcagt tcggttggga
    11641 aaccatcacc gaagcgctga agcagggcgg catcaccctg atgatggacc gtctctctaa
    11701 cccggcgaaa ctgcgtgctt acgcgctgtc tgaacagctg aaagagatca tggcacccct
    11761 gttccagaaa catatggacg acatcatctc cggcgaattc tcctccggta tgatggcgga
    11821 ctgggccaac gatgataaga aactgctgac ctggcgtgaa gagaccggca aaaccgcctt
    11881 cgaaaccgcg ccgcagtatg aaggcaaaat cggcgagcag gagtacttcg ataaaggcgt
    11941 actgatgatc gcgatggtga aagcgggcgt tgagctggcg tttgaaacca tggtcgattc
    12001 cggcatcatt gaagagtctg catattatga atcactgcac gagctgccgc tgattgccaa
    12061 caccatcgcc cgtaagcgtc tgtacgaaat gaacgtggtt atctctgata ccgctgagta
    12121 cggtaactat ctgttctctt acgcttgtgt gccgttgctg aaaccgttta tggcagagct
    12181 gcaaccgggc gatctgggta aagctattcc ggaaggtgcg gtagataacg cgcaactgcg
    12241 tgatgtgaac gaagcgattc gctgccatgc gattgagcag gtaggtaaga aactgcgcgg
    12301 ctatatgacg gatatgaaac gtattgcagt tgcgggttaa gtgcgcgctg atgccctcac
    12361 cccgaccctc tcccacaggg agagggagaa aacactcaag gccttctcct ggagaaggcc
    12421 ttgctattag ttacggtaca gcaccttaat gatgtggtaa ccgaactggg tgtgcagcgg
    12481 gccggtcggc tccagtaccg gacaagagaa cacaacttta tcgaacgccg gaaccatctg
    12541 accctggcgg aattcaccta aatcaccgcc gcgtttgcct gatgggcaaa tggagtgttt
    12601 cttcgccagc ttgccgaaat cggcaccgtt cttaatctgc tcgagaagat ccagagccag
    12661 tttctcttct tttacaagga tatgcagtgc tgctgctgtt tttgccatga tcgtgccttg
    12721 agtgaataat tgtcaggcgg cgattttagc atgatccgca gatgctaacg taattgtcag
    12781 tcagggaggg gctggtacat atatggcaaa agcccggaaa atccgggctt cgcaggtccc
    12841 tcaaggggag aaaacttagg gcctctatgg gctacaaagt cattatcggg gatggttatt
    12901 ttattgtcaa tttatgagcg atatggtatt gattgacttg atgaccgccc tctgtatgtc
    12961 tgcaaccaac tcatgtggca aacattgatt aagataccct ctgtaagtcc ttgctctatt
    13021 taatcggttt cgactaactt gttgtaccag gtcggctttg gcccaacgaa tctgctgatc
    13081 aaagaattgc aggccattga taacattact ggctatttca acatgcattc cacgatttaa
    13141 cttgtcatga tcgcgagtgg ttgataatgg aacgacgata catgcgttgc cattaatttt
    13201 tccgttgagc acgac
//
LOCUS       DAJTNA010000108        13100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTNA010000108 DAJTNA010000000
VERSION     DAJTNA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13100
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.109"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            8..1636
                     /locus_tag="OO161_002650"
     CDS             8..1636
                     /locus_tag="OO161_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR5463672.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTFEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1875..1934
                     /locus_tag="OO161_002651"
     CDS             1875..1934
                     /locus_tag="OO161_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5463673.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2251..2310
                     /gene="ibsE"
                     /locus_tag="OO161_002652"
     CDS             2251..2310
                     /gene="ibsE"
                     /locus_tag="OO161_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR5463674.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2430..3863)
                     /gene="hldE"
                     /locus_tag="OO161_002653"
     CDS             complement(2430..3863)
                     /gene="hldE"
                     /locus_tag="OO161_002653"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5463675.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LELRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3911..6751)
                     /gene="glnE"
                     /locus_tag="OO161_002654"
     CDS             complement(3911..6751)
                     /gene="glnE"
                     /locus_tag="OO161_002654"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5463676.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQVDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVHREIMTLPREGKTLQTKVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6774..8075)
                     /gene="ygiF"
                     /locus_tag="OO161_002655"
     CDS             complement(6774..8075)
                     /gene="ygiF"
                     /locus_tag="OO161_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5463677.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8318..8938
                     /locus_tag="OO161_002656"
     CDS             8318..8938
                     /locus_tag="OO161_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5463678.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            9002..10240
                     /gene="cca"
                     /locus_tag="OO161_002657"
     CDS             9002..10240
                     /gene="cca"
                     /locus_tag="OO161_002657"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5463679.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREVL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10397..11218)
                     /gene="bacA"
                     /locus_tag="OO161_002658"
     CDS             complement(10397..11218)
                     /gene="bacA"
                     /locus_tag="OO161_002658"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5463680.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11309..11677)
                     /gene="folB"
                     /locus_tag="OO161_002659"
     CDS             complement(11309..11677)
                     /gene="folB"
                     /locus_tag="OO161_002659"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5463681.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11782..12399
                     /gene="plsY"
                     /locus_tag="OO161_002660"
     CDS             11782..12399
                     /gene="plsY"
                     /locus_tag="OO161_002660"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5463682.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12412..>13100)
                     /gene="ttdR"
                     /locus_tag="OO161_002661"
                     /gene_synonym="dan"
     CDS             complement(12412..>13100)
                     /gene="ttdR"
                     /locus_tag="OO161_002661"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCR5463683.1"
                     /translation="LVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQ
                     VHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQS
                     LQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIM
                     LRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRL
                     GKPDEGYQVM"
BASE COUNT         3162 a         3682 c         3283 g         2973 t
ORIGIN      
        1 ctcctggatg tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt
       61 cgccaggctc tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg
      121 gcaaaaagtg gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc
      181 catcaatatg aatactctga agctggaagt cagccgctca accattgata gcctgattac
      241 gaaagatcgt atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt
      301 agaaggcatc gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt
      361 acgtatgttg gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac
      421 catgcatgag ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc
      481 agaagacctg tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca
      541 gacctcgaaa gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact
      601 gattcaggag acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt
      661 tgcggtgcgt gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga
      721 agcgtttatg acgtttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc
      781 acgtattgcg gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc
      841 tgaacgacaa attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt
      901 tgaggctgaa cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc
      961 caaccagacg aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc
     1021 gcgtgctaac cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg
     1081 ccagaccgca gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc
     1141 agaaaccaaa gcggttgaac tgaccgtgcg agcgaaagca gaaaaagaag ccgcagagat
     1201 gcaggcggcg gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc
     1261 agaagcacaa cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct
     1321 taaattcaaa ctggccttgt tgcaggcgct acctgcggta atagaaaaat ccgttgagcc
     1381 gatgaaatct attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc
     1441 tgcgggtgat gcaaacacgg gtaatgtggg gggcggaaac ctggcggaac aagcattatc
     1501 agccgctctc tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg
     1561 cgtttcaggc ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc
     1621 cgaaaacgta gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt
     1681 ttatatctgc gttccgctaa aaactgcaaa tgatcaggtc gttgcaacgt tttgcgtgac
     1741 cgctcgggga aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa
     1801 caatcgggga gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac
     1861 agaaggaaag ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt
     1921 tcgcagctta ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt
     1981 actttaggtt gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca
     2041 aaaagccggg gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg
     2101 cagcgttttg cgtgactgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg
     2161 gtccggcgct catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc
     2221 gaggaagggg ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg
     2281 ttgttactgt taagtttccc gacttactaa caactgatca gaggggggag aaatcctccc
     2341 ttacccttgt tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta
     2401 cgaagcgaga tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg
     2461 atgatgttag tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg
     2521 gcccagactt ctttactccc ggcgatctct tctggtttat agtcgccacc cttcaccagt
     2581 aaatctggca ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc
     2641 cagtcgaccg cttccagtgc gcccagcaca atcatacgca gttcgagtgg gttaacgggg
     2701 cgggaatccc ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg
     2761 tcacccagtt tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag
     2821 acaccgttgg tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc
     2881 agttcctctt cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc
     2941 agctcgatcg gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca
     3001 ttggcaaaga agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg
     3061 ccaatcaccg tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata
     3121 tgcagcggcg ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac
     3181 agagccgaga gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg
     3241 gtcttacatt taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg
     3301 ccgcggtagc gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta
     3361 cgcgccagtt ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc
     3421 accagcgcgc caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca
     3481 acgccttcga aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc
     3541 cgtaatttgg tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg
     3601 tcggccagag atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg
     3661 cgtgcattag caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt
     3721 tcttcgatgg tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggtg
     3781 ggaccgtacc agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca
     3841 aactctgaca gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata
     3901 ataccatact tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct
     3961 ctgcggtgaa gcaatcctcc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa
     4021 gttcatcgcg cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa
     4081 tgtcgttttg cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg
     4141 gtttttcatg agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc
     4201 cgccttcatc ggctttgata tcaaagcgat tacgatgttt attgccgaga tgagcgcgca
     4261 ttttctcgcg catttcccgc actttcgttt gcagagtttt accttcacgc ggcagcgtca
     4321 taatctcgcg atgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactacac
     4381 gcgcacgcac cagcgcctga tgctcccacg tccaggcctc gtttttctga taatcagcaa
     4441 atgcttctgc ggatgtcacc agcattcccg ccgccccgga cggacgcagt cgtgcatcca
     4501 cttcatacaa aatgccggaa gaggttcgcg tattgaacag atgcataatg cgttgcgcca
     4561 gacgcagata aaactgtcgc ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg
     4621 ggcaatcatg gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca
     4681 gcttgccgta gccgaccacc gcaaaaccac ggccttcgcg atcgttgagg tgatttggct
     4741 taccgtagcg ggccaccatt tgaacccacg cctgctgaac gacggcatct atcatagcct
     4801 ctgccagcca ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg
     4861 ctgcggcgat gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct
     4921 cttcgtcatc ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg
     4981 tcgccgtcgg ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac
     5041 gcgccagctg gctggcaatc atcggcgatg cggcacacag agaaatcaaa tgtttaagcg
     5101 ccgcagggaa ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca
     5161 aggcggtaat gcgcgacagc gtaacggcag cgtcttcacg cgcacagaca tcacttagca
     5221 gatgtggcat cagatggtcg agcacctgac gtcctcgcgg cccgatagta cgcttatcca
     5281 gctctttgcg gaaatcagca atcagcgtta ggacctgttt gcgatcatcc tcgctaagat
     5341 gcgccagcac tggcgtagtg tcatctacct gcaacgcatc ctgccacagc tcacgccact
     5401 gttccgacag cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca
     5461 cccggcgcac attggtcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa
     5521 agtccatcgc ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtttgggttt
     5581 gttcgtcgtt aatgctttgc agcaggtttt ccagacgccg caggaacaga tatgccactc
     5641 gcaattgttc agcatcgttt tcagaaagca gatgcagcgc ggcaatgacg ctgagcgttg
     5701 gcagtaaaga gcgcgactgc agcgacggtt cgcgcccacc gcgaatgagc tggaacacct
     5761 gaacgataaa ttcaatttcg cgaatgccgc ctgcgccgag tttgatattg tcggtcaaac
     5821 cacgtcgacg cacttcacgg gcaatcatcc ctttcatgtt gcgcagcgac tgaatcacgc
     5881 tgaaatcgat gtaacgacgg aaaacaaacg ggcgcagcat cgcacgcaac tcgttagcat
     5941 agacgccttc gctatcgccc ataatccgcg ccttgaccat cgcgtaacgc tcccagtcgc
     6001 gcccctgctc ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt
     6061 cgccaaacgg acgcagccgc atatccacgc gatagacgaa gccatcctgc gttggttgat
     6121 ccaacacttt aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc
     6181 ggcgtccgcc ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatctgagg
     6241 agaaattcag ttccccaccg cccagcttac ccatgcctaa aatcagcagc ggttgcgctt
     6301 cgccctgcgc attgcacggc gttccccact cgcggcagca agcgtcatac agccagtcac
     6361 gcgcggcgac aatcagcgtc tccgccagat ggctgagctg ctgcaatatg ctctcttcag
     6421 taaccaaagc cagcgtttgc gcccaggcga tgcgcaccat aatgcgccgc cggaatagcc
     6481 gcagctcgcg cattaacccg gcttcgtcac tcacattgct gagcgcctcc tgcaaccatg
     6541 ccgcgtaatg ctgccattca tcagcctgcg gcgattggct ttccagttcc gtcagccact
     6601 ctggatgcgc aatcacgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct
     6661 gtgcgctaag tgatttctcg gctaaaggct ctggcagccg ctcaacaacc gtctgccagt
     6721 actgctgtaa cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt
     6781 tttccgctgt gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgttcaatt
     6841 tcaatgcgtt gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggct
     6901 tgcgcgacga caggatcata gtaacccgcc agcaacagta ttgagtcaat atcacgcgtc
     6961 agacgtggca actggtcacg gtagcgatcg cccaacggct ggcagaaaac gcttttcagt
     7021 tcagcggcat ggcgggaaag atggatatcg gcaaagcgtt tgaaggaatc gctcatttta
     7081 ctctgcgctt tggcatctaa aaacggctgc caggctttgc ttaccagcca ttcggtcaac
     7141 gccagcttcg ccattgccgt tttggtagag tagaccgccg tcacggcaga aaccgcagaa
     7201 gcgatggtcg cttcgcattg agtcagcaaa tcacgtaagt gagtgctcgc tttgcgcggc
     7261 acaataccac cgaacagcat cagtgtatga cggaccagac caatggctgc cagcacctgt
     7321 tctttcgccg catcgttgcc gcgtacccac agttcttcat gatactgcca ttgcgctaac
     7381 gccagctcga gcgccgcttc cagcccctgt tccacatcgg cttttgccgc aacatgcaaa
     7441 atggtggtcg gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc
     7501 gctttgctca ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc
     7561 agcaccgcgc gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca
     7621 aattcccccg ctttcacctc cccctgatcg agagcgattt caattcggct gtcatcgacc
     7681 gccaccagcc atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg
     7741 agatcggcgg gcaattcgcc gttcggccag acttccgtcg gtaactgcgc caggtcgagc
     7801 gtcggttcgc tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt
     7861 cctgcaactt tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata
     7921 tcgtgcccac gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg
     7981 tcatggtgct cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga
     8041 ttaacaataa actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac
     8101 ataagtgacg atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc
     8161 gccaaattgc catgcaacag ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     8221 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     8281 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     8341 attaacttta ctcgcactta gcgccactgc cgtctcacac gctgaagaaa cgcgctatgt
     8401 ttctgatgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     8461 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     8521 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     8581 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     8641 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     8701 gcagagcgac agcgtgatca acggattaaa agaagaaaat cagaaattga aaaacgagct
     8761 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     8821 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     8881 tctggtactg ccacacctga tcccaacgcg caaacgcaaa gatcgctgga tgaactaaat
     8941 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     9001 cgtgaagatt tatctggttg gtggtgctgt tcgggatgca ttgttagggc taccggtcaa
     9061 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     9121 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     9181 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct atgccgcacc
     9241 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     9301 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     9361 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgttctgc gcgtggcgcg
     9421 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     9481 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     9541 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     9601 cggtgcactt cgcgttttat tcccggaaat tgacgccctg tttggcgttc cggcccctgc
     9661 caggtggcat ccggaaatcg acaccggtat tcatacctta atgacgctct caatggcggc
     9721 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     9781 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     9841 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     9901 ggtggctgag tttcacgatc tcatccacac cttcccaatg ctgaacccaa aaaccatcgt
     9961 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
    10021 gaccagcgag gctgacgtac gcggcagaac cggttttgaa tcagcggact acccgcaagg
    10081 ccgttggttg cgcgaagcct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
    10141 agcgggattt aaaggtgtgg agattcgcga ggtgctgacc cgacggcgga ttgcggcggt
    10201 agccagttgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttaac
    10261 acgaaccatc ccctcgcccc ttcggggaga gggtgagggg aacagaccag caccggtgcg
    10321 aacaatcacc cccaccccgg ccctctccct acagagcgag ggagaaaacc aggcctgctc
    10381 ccgaaccttt tctcaatcaa aagaacacga cgtacaccgc agccgccaca ataaagcgat
    10441 aaatggcgaa cgggataaac gaaatgcgct taatcaattg caggaaggtt ttaatcgcta
    10501 tcagcgccac cacaaaagcg gtgataaacc caacggcaaa catcgggata tcgccggttg
    10561 tcaggaagcc ccagcttttg tagagatcga gcgccgttgc gcccatcatc atcggtaccg
    10621 ccagcaggaa cgagaactcg gaagcagcgt aacggctcac gcccatcagc atcccacctg
    10681 aaatggtggc cccggaacgg gagaaacccg gccacagcgc cagacactgg aaacagccaa
    10741 tcataaacgc ctgacgatag gtcatatcat caagacccgg cgcacgcggc tctttcggct
    10801 tcaggcattc ggcggcaatc agcaacaaac cgccaacgac cagtgcatac atcacattta
    10861 tcgggttaaa caatgactta atcgtgtcgt ggaacaacag ccccagtacc accgccggaa
    10921 tcatccccag caaaatgtgg atcagcgtta aacgaccttt gctttctcct tcgtgctgca
    10981 acgggcggcc aaagtggatg ccaatcaggc caaacagacg ccgccagaac atcactacta
    11041 ccgccagaat tgatcctaac tggatcacaa cttcaaaggt tttcgccgtg tcgccctcga
    11101 accccaacaa gtgaccgaca ataatcatat ggcccgtgct ggataccggc agaaattctg
    11161 tcaatccttc gaccacaccc aatattgccg ctatcagcag cgagtgcata tcgctcatca
    11221 ataaacccct aaattattaa aatgtaccgc ttgtccggac tactgcagta agaccaggtt
    11281 ataaccgttt ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct
    11341 caatgattac accaacattc gccgcccgcg ccactgcgcc tggcttgctg agtttgatgc
    11401 gcacccacgg cgagttgaag cgtgctaaca gcagctccgc cacctcttca gccacgcgtt
    11461 ccaccagcgc aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca atgtcagcgt
    11521 aactgaggca atccgccaca tcatcacttt tcgccgcttt acggttatcc catgccattt
    11581 cgatatcgaa cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg
    11641 tgattaccga aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct
    11701 aaccggatac cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga
    11761 attttcaaaa cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac
    11821 ctctgcggct ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg
    11881 cgaaccagcg gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga
    11941 gcagccgtag cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg
    12001 tatgaattag gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga
    12061 cacatctggc ccgttttctt cggatttaaa ggaggaaaag gcgttgctac cgcttttggt
    12121 gccatcgcac ccattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc
    12181 gtgctattga gcggatactc gtcgctggga gcgattgtca gtgcactgat tgctccgttt
    12241 tatgtctggt ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc
    12301 ctgctgcgtc atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg
    12361 acgaaattca aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga tctacatgac
    12421 ctgatagcct tcatcgggct tgcccagccg ttgctggcac catgccgcca gaaattccac
    12481 gcagacacgt aatttcatgc tgcgatagag cggctcccgg taaacagccc agatattggc
    12541 gctttgtgca tactccggca atacttgcac cagtttgcca ctctccagaa acggcaacac
    12601 atcccactcg gaacgcagca taatcccttt gccctccagc gcccattgca acactatctc
    12661 gccgctattg gaggaaagat gcccgctaac cttcaccgat tttttctcct gaccgttccc
    12721 caactcccat attccgtggg tcatatcgcg ctctttggtc accagacagt catgacgact
    12781 taattcctgt aatgattgtg gctgcggata tttttgcaga tactcaggtg ctgcgcataa
    12841 tattctttta tttttcgtta acagatgcgc aatataataa tcaggaattt cgtcattaat
    12901 acgaatatcc agatcaatat tatcctgcac taaatcaatt tgccgatcga acaattcaaa
    12961 atgcacctgt aactcaggat aattgcgcat aagttcggta atggctggcg caatatggct
    13021 gcgcccaaaa ccaaaactgc aaccaatacg aatcatcccc tccgggcgcg ttttgatttg
    13081 cgtgacgtca tcgaccagtc
//
LOCUS       DAJTNA010000109        13099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTNA010000109 DAJTNA010000000
VERSION     DAJTNA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13099
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.110"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..724)
                     /locus_tag="OO161_002662"
                     /pseudo
     CDS             complement(1..724)
                     /locus_tag="OO161_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150766.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            1191..2423
                     /gene="mdtM"
                     /locus_tag="OO161_002663"
     CDS             1191..2423
                     /gene="mdtM"
                     /locus_tag="OO161_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCR5463684.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGATTISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2464..3744
                     /locus_tag="OO161_002664"
     CDS             2464..3744
                     /locus_tag="OO161_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCR5463685.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            3860..5013
                     /locus_tag="OO161_002665"
                     /pseudo
     CDS             3860..5013
                     /locus_tag="OO161_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            5023..5790
                     /locus_tag="OO161_002666"
     CDS             5023..5790
                     /locus_tag="OO161_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463686.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            5787..6044
                     /locus_tag="OO161_002667"
     CDS             5787..6044
                     /locus_tag="OO161_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCR5463687.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            5998..6969
                     /locus_tag="OO161_002668"
     CDS             5998..6969
                     /locus_tag="OO161_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCR5463688.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNLIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            7037..8215
                     /locus_tag="OO161_002669"
     CDS             7037..8215
                     /locus_tag="OO161_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5463689.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGTFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(8228..8782)
                     /gene="kptA"
                     /locus_tag="OO161_002670"
     CDS             complement(8228..8782)
                     /gene="kptA"
                     /locus_tag="OO161_002670"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5463690.1"
                     /translation="MAKHNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            9032..9715
                     /locus_tag="OO161_002671"
     CDS             9032..9715
                     /locus_tag="OO161_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463691.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9712..10173
                     /locus_tag="OO161_002672"
     CDS             9712..10173
                     /locus_tag="OO161_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5463692.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10186..11358
                     /gene="iadA"
                     /locus_tag="OO161_002673"
     CDS             10186..11358
                     /gene="iadA"
                     /locus_tag="OO161_002673"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5463693.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHIPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11423..12334
                     /gene="hypT"
                     /locus_tag="OO161_002674"
                     /gene_synonym="qseD"
     CDS             11423..12334
                     /gene="hypT"
                     /locus_tag="OO161_002674"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5463694.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12327..12719)
                     /gene="iraD"
                     /locus_tag="OO161_002675"
     CDS             complement(12327..12719)
                     /gene="iraD"
                     /locus_tag="OO161_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5463695.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12716..12799)
                     /gene="idlP"
                     /locus_tag="OO161_002676"
     CDS             complement(12716..12799)
                     /gene="idlP"
                     /locus_tag="OO161_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5463696.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         2952 a         3322 c         3361 g         3464 t
ORIGIN      
        1 tctaatgcct gcatttgctc tgccgaaagt ccggtatatc gttgtatcca ttcaggatcg
       61 gccccattct gcaacaacaa tcggaggagg ctccgttccc cttctacttt cccttctttc
      121 ctccccctct cccttcccaa cagccatcca tcattatgaa ttcgctctgc aattgtcatt
      181 attcgctccc tgtggtgtgg cattcgcctg gtaagttcgc tgataaacgc cttaaaacgc
      241 gcttcatcgc cagtcagtaa aatgtaattt aacagcgcag ttatctggct gtcattagca
      301 cactctgtaa ccagtaatac taccagttgg tcaataagcc ccatcaaatc gcgctggcga
      361 atatgctttt ggatcaactc caacagggcg actctgcgat gctgcacaat ctcgtcgtct
      421 ggcacgacag tgacatccac cagcgggaac gctgcgctat aaagtttccg tgcggtagtc
      481 ggggcggcaa attcgtccag ccagcacagg gaccaggggt aaggactacg gctaccgtga
      541 taaaacagca tcgggataac cagcggtagc ggccggcgtt tatcatgctc gatatggcgc
      601 tgcatcaccg ccatggaata tcgcatcagg cgaaatgcca tatgaatatc cgcgcggtgt
      661 cagggtgagt gagaaaggat ttaaataatg cgtcatgtgg cgtgctggtc gtgaagtttg
      721 tcatggcgat ccgtcacctg tagataagat ggcccgactt tagcgactaa caaaaccatt
      781 ctcacccggc aatttctcgt tccccgaggt gctttccagg cgtctgcaaa cgatacattt
      841 ttccggaaac aggctcgcgt tttacatttc ctgtacccgg cagatattcc cctttccacc
      901 gcgctctttt gattaaccgc aaattttaat tacacttaag gtgtatattt tctatgcaac
      961 ccatcaatta aagaggtgta atgtgctgat gactatttgt aatcgttata cattctgacc
     1021 tggagtcaga aggtatttct ctgtctgtgt gttcacaggc agtgtggttg attacatgaa
     1081 attcagtaca tttgcagtct cgttgccctt ctcgcctgcc tttcgtcatt accgacggta
     1141 ttgaatttcg ttttccccgt tggggttctc cgaacaagga gttgtttgtt atgccacgtt
     1201 tttttgcccg ccatgccgcc acgctgtttt tcccgatggc gttgattttg tatgactttg
     1261 ccgcgtacct gtcgacggat ctgatccagc cggggatcat taatgtggtc cgtgatttta
     1321 atgccgatgt cagtctggcc ccggcagccg tcagtctcta tctcgcgggc ggtatggcgt
     1381 tacagtggct gctggggccg ctttccgaca ggattggccg caggccggtg ctgattaccg
     1441 gggcgctaat atttaccctt gcctgcgctg cgacaatgtt cacaacgtct atgacacagt
     1501 ttcttatcgc gcgtgcaatt cagggcacca gtatctgttt tattgccacc gttggttatg
     1561 tcacggtgca ggaggcgttc ggccagacaa aagggatcaa gttgatggcg attatcacct
     1621 ccatcgtact gattgcgccg attattggcc cgctttccgg cgcagctctg atgcactttg
     1681 tgcactggaa agtccttttt gccatcattg cagttatggg gtttatctca tttgttggct
     1741 tactgctggc gatgccagag acggtgaagc gcggcgcggt tccatttagc gccaaaagcg
     1801 tctttcgcga ttttcgtaat gtcttttgca atcggctgtt cctctttggc gcaacaacca
     1861 tctctttaag ctatatccca atgatgagtt gggtggctgt ctcgccggtg atcctgatcg
     1921 acgcgggcag cttaacaact tcgcagttcg cctggacaca ggttccggtg ttcggcgcgg
     1981 tgattgtcgc gaatgccatc gtggcgcgtt tcgttaaaga tccgaccgaa ccgcgcttta
     2041 tctggcgtgc tgtacccatt caactggtcg gcctcgcgct gttgattatc ggcaatctgc
     2101 tgtcaccgca cgtctggctg tggtcggtgc tgggcaccag tttgtatgct ttcgggattg
     2161 gtttgatctt cccgacctta ttccgcttta cgcttttttc caataactta ccgaaaggga
     2221 ccgtctccgc atcgctgaat atggtgatcc tgatggtgat gtctgtctcg gtcgaaatcg
     2281 gccgctggct gtggtttaac ggcggtcgct tgccgtttca tctgttagcc gtcgtggcgg
     2341 gcgttatcgt cgttttcacc ctggcaggat tgcttaatcg cgtgcgccaa catcaggctg
     2401 ccgagctggc ggaggagcgg tgatttttgc gcgatcctgc cgtcaggctc tattcttaac
     2461 gttatgaata aactcattga actcagacgc gccaaaatgt tggcgctctc tttactgctt
     2521 atcgccgctg ctacctttgt cgttacgctg tttttgccgc ccaatttttg ggtgagcggc
     2581 gtgaaggcga ttgctgaagc ggcgatggtc ggcgcgctgg cggactggtt tgcggtggtg
     2641 gcgctgtttc gccgcgtgcc gattccgatc atttctcgtc atacggcgat tatcccgcgt
     2701 aataaagacc ggattggcga aaatctcggt cagttcgtgc aggaaaaatt tctcgatacc
     2761 cagtcgctgg tggcattgat tcgacgtcac gaaccggcgt tgttgattgg caactggttt
     2821 agtcagccgg agaacgcccg ccgcgttggt cagcatctgt tgcagatcat gagcggtttt
     2881 cttgaactga ccgatgatgc gcgtattcag cgcctgctta agcgcgcggt ccatcgggcg
     2941 attgataagg tcgatctttc cggaaccagt gcgttgatgc tggagagtat gaccaaaaac
     3001 gatcgtcatc aggtgctgct cgatacgctg atcgcacagt tgatcgccct tctccagcgc
     3061 gataaatcgc gcaagtttat tgcccagcaa attgttcgct ggctggagag cgagcatccg
     3121 ctgaaagcca aaattttgcc tactgaatgg ctgggcgaac atagcgcgga gttggtttct
     3181 gacgcggtga attctttgct tgatgatatc agccgcgatc gtgcgcatca gatccgtcat
     3241 gcgtttgatc gcgccacttt tgccctgatc gacaagctga aaaacgatcc ggaaatggca
     3301 gcgcgagccg atgccgtaaa aagttatctg aaagaagatg aagcttttaa ccgctatctc
     3361 agtgaattgt ggggggattt acgggaatgg ctgaaagcgg atatcaacag tgaagattct
     3421 cgtgtgaaag aacgtatcgc gcgggcgggt caatggtttg gcgaaacgtt aattgccgat
     3481 gatgccttgc gggcatcgtt aaatggtcac ctggaacaag ccgcgcaccg cgtcgcgcct
     3541 gagttttccg cattcctgac gcgccacatc agcgatacag taaaaagctg ggatgctcga
     3601 gatatgtcgc ggcaaatcga gttaaatatc ggcaaagatc tgcagtttat ccgtgtcaac
     3661 ggtacgctgg ttggcggttg tattgggcta attttatatt tgttgtcgca gctcccggcc
     3721 ttgttccccc tcagcaattt ttagaaatcc atgaaaaact aagtggctaa ccgcgagaga
     3781 gatcaaataa accatactaa aagaatggta ataatgtggc ctcttttcat tatctcccgt
     3841 ggtacgggga aggaaaatca tgtcacttat caccgatcta cccgccattt tcgatcagtt
     3901 ctctgaagct cgccagaaag gctttctcac cgtcatggat ctcaaggagc gcggcattcc
     3961 gctggttggc acttactgca cctttatgcc gcaagagatc ccgatggcag ccggtgcggt
     4021 tgtggtttcg ctctgttcta cctctgataa aaccattgaa gaagcggaga aagatctgcc
     4081 gcgcaacctc tgcccgctga ttaaaagcag ctacggcttc ggcaaaaccg ataaatgccc
     4141 ctacttctac ttttcggatc tggtggtcgg tgaaaccacc tgcgacggca aaaagaaaat
     4201 gtatgaatac atggcggagt ttaagcccgt tcatgtgatg cagttgccga acagcgttaa
     4261 ggacgatgcc tcgcgtgcgt tatggaaagc cgagatgctg cgcttgcaaa aaacggtaga
     4321 agaacgtttt gggcacgaga ttagcgaaga tgctctgcgc gatgccattg cgctgaaaaa
     4381 ccgcgaacgt cgcgcactgg ccaattttta tcatcttggg cagttaaatc ctccggcgct
     4441 tagcggcagc gacattctga aagtggtcta cggcgcaacc ttccggttcg ataaagaggc
     4501 gttgatcaat gaactggatg caatgaccgc ccgcgttcgt cagcagtggg aagaaggcca
     4561 gcgactggac ccgcgtccac gcattttaat caccggctgc ccgattggcg gcgcagcaga
     4621 aaaagtggtg cgggcgattg aagagaatgg cggctgggtt gtcggttatg aaaactgcac
     4681 tggggcgaaa gcgaccgagc taatgcgtgg cggaaacggg cgatgtctac gacgcgctgg
     4741 cggataaata tctggcgatt ggctgctcct gtgtttcgcc gaacgatcag cgcctgcaaa
     4801 tgctcagcca gatggtggaa gaatatcagg tcgatggcgt agttgatgtg attttgcagg
     4861 cgtgccatac ctacgcggtg gaatcgctgg cgattaaacg tcatgtgcgt cagcagcaca
     4921 acattcctta tatcgctatt gaaacagact actccacctc agatgtcggg gcagctcagt
     4981 acccgtgtcg cggcctttat tgagatgctg taaggagtgg cagtgacata ttcgattggc
     5041 attgattctg gttctaccgc caccaaaggg atcttactag cagacggcgt gattacgcgc
     5101 cgtttcctcg ttccaacacc ctttcgtccg gcaacagcaa ttactgaagc ctgggaaact
     5161 ctgcgcgaag ggttagagac aacgccgttt ctgacgctca ccggctatgg acggcaactg
     5221 gtggattttg ccgataaaca agtgacggaa atctcctgtc acgggctggg cgcacggttt
     5281 cttgcgccag caacgcgcgc ggtaatcgac atcggtggtc aggacagcaa agtgattcag
     5341 cttgatgatg acggtaacct gtgcgatttc ctgatgaatg acaaatgcgc ggcgggcacc
     5401 gggcgtttcc tggaagtgat ctcgcgcacg cttggcacca gcgtcgagca actcgacagc
     5461 attaccgaaa atgtcacgcc gcatgccatc acgagtatgt gcacagtgtt tgccgaatca
     5521 gaagtgatca gcctgcgctc agcgggcgtc gcgccagaag cgattctcgc cggagtgatt
     5581 aacgcgatgg cgcggaggag tgccaatttc attgctcgtc tctcctgtga agcgccgatt
     5641 ctgtttactg gtggcgttag tcattgccag acgtttgccc ggatgctgga atctcacctg
     5701 ggaatgccgg taaataccca tcctgatgcg caatttgctg gcgcaattgg cgcggcggta
     5761 attggtcaac gagtgaggac acgccgatga aagagttttt atttttattt cactccacag
     5821 tcggcgtcat acaaacccgc aaagcgttgc aggcagcggg catgactttt cgcgtcagcg
     5881 atattccccg tgatttacgc ggcggctgcg ggttatgcat ttggctgacc tgtccccccg
     5941 gcgaggaaat acaatgggtg atccctgggc tgactgagtc aatttattgc cagcaggatg
     6001 gtgtctggcg ctgcattgca cattaccggg tttctccgcg ttaaggattc attaaggtcg
     6061 ctacgtatac ttctcgatat cttattgatt tgccttaaag ggattaccgt gacaaaaagt
     6121 atttcgttaa gtaaacgcat ctccgttatt gtgattcttt ttgccattgt ggcagtgtgt
     6181 actttcttcg tgcaatcttg cgcccgtaaa agcaatcatg cagcatcatt tcagaattac
     6241 cacgccacca ttgacggcaa agaaattgca ggcataacga ataatatctc ttcgctgacc
     6301 tggtcggcac aaagcaatac tctttttagc actatcaaca aacctgccgc cattgttgaa
     6361 atgaccacca atggtgattt tatccgcaca attcctctgg attttgttaa ggatctcgag
     6421 acgatcgaat acattggcga taatcagttt gtcattagcg atgagcgcga ttacgccatt
     6481 tatgtgattt cactgacccc aaactcggaa gtaaaaatcc tcaaaaaaat aaaaatcccg
     6541 ctgcaagact ccccaaccaa ttgcggtttt gaagggttgg cttattctcg ccaggatcac
     6601 actttctggt tttttaaaga gaaaaatctg attgaggtct acaaagttaa cgggctgtta
     6661 agtagcaatg aattacacat cagcaaagac gaggcactac aacggcaatt tactttagat
     6721 gatgtgtccg gcgcagaatt taaccagcaa aaaaatacgc ttctggtgct gtctcatgaa
     6781 tctcgcgcac tccaggaagt caccctggtt ggcgaggtta tcggtgaaat atcactaacc
     6841 aaaggtagtc gaggtttgtc gcataacatc aaacaggccg aagggattgc gatggatgcc
     6901 tctggtaata tttatatcgt tagcgaacca aatcgttttt atcgctttac ccctaaatca
     6961 tcgcattaac aggctggcat atttcacctt tttgttatta acttgttaac aacaaaaatg
     7021 acatacaagg ttaaacatgc cttcgtcctc gcatcccgta gaacgctttt ctttcagcac
     7081 cgcgcttttc gggatgctgg ttctgaccct aggtatggga ttaggtcgct tcctttatac
     7141 gcctatgttg cccgtcatta tggcggaagg ggcgttttca tttagccagc tctcgtggat
     7201 tgccagcggc aactatgcgg ggtatctggc tggcagtctg ctattttcgt ttggcacatt
     7261 tcaccagccg tcgcgcctac gcccatttct gttggtttct gccctggcga gcgggttgtt
     7321 gatcctcgca atggcatggt tgccgccatt tattctggtg ttattgattc gcttcctggc
     7381 gggtgtcgcc agcgccggta tgctgatttt tggttcgacg ctgattatgc agcacacgcg
     7441 ccatcctttt gtgctggcag ctttgttttc tggcgttggc attggcattg cactgggcaa
     7501 cgaatatgtt ctggcaggcc tgcattttgc cttctcctcg caaacgttat ggcaaggcgc
     7561 cggagcgctt tctggcatga tgctgattgc acttacgctt ttaatgccct cgaaaaaaca
     7621 cgctatcgca ccaatgccat tggcaaaaac ggagcaacag ataatgagct ggtggttact
     7681 ggctattctg tacggccttg cgggatttgg ttatatcatc gtcgcgactt acctgccgct
     7741 catggcgaaa gatgcaggct caccattgtt aaccgcccat ctctggacgt tagtcggctt
     7801 atcaattgtg ccaggctgct ttggctggct atgggcagca aaacgttggg gggctttgcc
     7861 ttgcctgacg gcgaatttgc tggtgcaggc tatcagtgtg ctgcttactc tcgccagcga
     7921 ctctcctctc ttgcttatca ttagtagtat cggttttggc ggtaccttta tgggcacgac
     7981 atctctggtg atgactatcg cccgccaact gagcgttccg ggaaatctca atcttttagg
     8041 ctttgtgacg ctcatttacg gcattggaca aatcctcggc ccggcgctga ccagtatgct
     8101 cggcaatgga acatcggcgc ttgccggcgc cactctctgc ggcgcggcgg cgttgtttat
     8161 tgctgcgctt atcagcacag tacaattatt taagctgcaa gtggtcactt catgaggagt
     8221 gtttccctta ccactcaaga aattcgaccg caaccgttga tgtcagccag actccgtttt
     8281 ccgcttgcca gaaaggaatg cctcgtttcg ccatttcctg tgctttgacg gttaagacaa
     8341 ccggcgatcc gtgtcttgct cccactttgc gtgccgttgc ttcatcagca gagagatgca
     8401 cgtaatgacg atcccctgca atcagcccct gtttttttat ctcgtcaagg aaacggcttg
     8461 ccgtaccgtg gtagagaaac tgtggcggtg ttttttcggc aaaggaaata gcgacctgcg
     8521 aagtcgaatg cccctgaacc gcgcggatgc acctaccatc actggaataa ctaaaacgct
     8581 ttttatcgct ggttgcgacc acggtatcca gcaatgcacg ggttaatcgc ttgcccgctt
     8641 tttgggcaca aagtatgagc ttatcgatat ccgcccatcc ttcacggtcc agtacgatac
     8701 caatcgcctc cggtttgtgt cggaggacaa aacttaaaaa cttgctcgta tccgccagct
     8761 ctttttcgtt gtgctttgcc atctcgtttt cgtccctgga ttttctgttt ttatttacca
     8821 atgtcattgt cactgggttt atgaattttc tgcaactcaa tcctgcgcag aaccttttcc
     8881 acacagcgta atgtttcccc tatgcaaaaa acgcatagcc agaaacatac ttgcattttc
     8941 cccataccca catggctgat aaggtttttc agttccagga attttcctgg ctcaagcaac
     9001 acacaaacac aaaaaaacaa tggggattgt tatgacacaa caaggggatg ctgtagccgg
     9061 ggagctggcg acagaaaaag tgggcattaa agggtatctg gctttttttc tcaccataat
     9121 ctttttttcc ggtgtttttt ccggaacaga cagctggtgg cgcgtttttg atttttccgt
     9181 tctgaacgga tcgtttgggc aactgcctgg tgcgaacgga gccaccacct catttcgcgg
     9241 cgcgggtggt gcgggggcta aagatggctt tctctttgcg ctggagctgg caccgtcagt
     9301 cattctctct ctggggatta tttcaattac cgatggtctt ggtggattac gcgccgcgca
     9361 gcagttaatg acacctgtgc taaaaccgtt gttgggcatt ccagggatct gctcgctggc
     9421 gctgatcgct aacttgcaaa acactgatgc cgccgccggt atgaccaaag agctggcgca
     9481 ggaaggtgaa attaccgaac gcgataaagt gattttcgcc gcatatcaaa ccagcggtag
     9541 cgccatcatc accaactact tctcttccgg cgtcgcggtg ttcgcctttc tgggcacgtc
     9601 cgtaatcgta cctttagccg tcatcctggt gtttaaattt gtcggagcca acattttgcg
     9661 cgtctggctt aacttcgaag aacgtcgcaa cccaacgcaa ggagcgcaag catgacaact
     9721 caggtccgca aaaacgtcat ggatatgttt atcgatggcg cgcgccgtgg ctttactatc
     9781 gccacgacca acctgctgcc aaacgtggtg atggcgtttg tcatcattca ggcgctgaaa
     9841 attaccggcc tgctcgactg ggtggggcat atctgtgaac ccgtaatggc gctgtgggga
     9901 ttacccggtg aagcggcaac cgtcctttta gccgcattaa tgagtatggg tggcgcagtc
     9961 ggtgttgccg ccagtctggc aaccgctggc gcattaaccg gtcacgatgt caccgtttta
    10021 ctgcccgcga tgtatttaat gggcaacccg gtgcaaaatg tcggtcgctg cctggggaca
    10081 gccgaagtga atgccaaata ttacccgcat atcattacgg tctgcgtgat taacgcattg
    10141 ctgtcgatct gggttatgca gcttattgtt taacaaggag ttaccatgat tgattatacc
    10201 gcagccggtt ttaccctgct gcagggagcg catttgtatg cgccggaaga tcggggaatt
    10261 tgcgatgtcc tcgtcgctaa cggcaaaatt atcgccgttg ccagcaatat cccttctgac
    10321 attgtaccga actgcacggt tgtcgatctc agtgggcaga tcctctgccc aggttttatt
    10381 gatcaacacg tccatttgat tggcggtggc ggcgaagcag gtcccacgac gcgcacgccg
    10441 gaagtggcgc taagtcgcct gacggaagcg ggcgtcacgt cagtggttgg tctgctgggc
    10501 accgactcta tctctcgcca cccggaatcc ctgctcgcca agacccgtgc gctcaatgaa
    10561 gaaggcatca gcgcctggat gctgaccggc gcttatcata tcccttcccg cactattacg
    10621 ggttccgtgg aaaaagacgt ggcgattatc gatcgtgtga ttggcgtgaa atgcgccatc
    10681 tctgatcacc gttctgccgc accggacgtt tatcacctgg ctaatatggc ggcagaatcc
    10741 cgcgttggcg gtttgctcgg cagtaaacct ggcgtcaccg tgttccacat gggcgacagt
    10801 aaaaaggcgt tacagcctat ttatgacctg ctggaaaact gcgatgtgcc gatcagcaag
    10861 ctgctgccga cccacgttaa ccgcaacgta ccgttgtttg agcaggcgct ggagttcgcg
    10921 cgcaaaggcg gcaccatcga tatcaccagc agcattgacg aaccggtcgc ccctgccgaa
    10981 ggtattgccc gcgccgttca ggcgggtatt ccgctggcac gcgtcaccct cagctccgac
    11041 ggcaacggta gccagccgtt cttcgatgac gaagggaatt taacccatat cggtgttgca
    11101 ggttttgaaa cgttgctgga aaccgtgcag gtgctggtca aagactatga tttcagtatc
    11161 agcgatgccc tgcgcccgct caccagtagc gtagccggtt tccttaacct gagcgggaaa
    11221 ggcgaaattc tgcctggcaa tgacgcagac ttactagtca tgacgccaga attgcgcatt
    11281 gagcaggtat atgctcgcgg caaactgatg gtcaaagacg gcaaagcctg cgtgaaagga
    11341 acgtttgaaa cggcttaaac ggttgtcagc cgccctaatg gacgtagaat gcccgcaagg
    11401 gcggctgaca gagtaaaacg taatggatga ctgtggtgcg gttttgcata atattgaaac
    11461 caaatggctt tatgattttc tgaccctgga aaaatgccgc aatttttccc aggcggcagt
    11521 cagtcgtaac gtctcgcaac cggcattcag ccgccgcatc cgcgcgctgg aacaggcgat
    11581 tggcgttgaa ttgtttaacc gccaggtgac gccgctgcaa ctctcagaac aagggaaaat
    11641 cttccattcg cagatccgcc atctgttgca acagttagaa agtaaccttg cagagctgcg
    11701 tggcggcagc gattacgcgc aacgtaaaat caagatagcc gctgcacact ctctttccct
    11761 cggtctgtta ccgtccatta tcagccagat gccgccgctc tttacctggg cgattgaagc
    11821 tattgatgtc gatgaagcag tcgataaact gcgtgaaggg caaagtgact gtattttttc
    11881 ctttcacgac gaagatttgc tggaagcacc gtttgaccac attcgcttat ttgaatctca
    11941 attgttcccc gtttgcgcca gtgacgaata cggagaagca atttttaacc tcgcgcagcc
    12001 acactttccg ttactgaatt acagccgcaa ctcctacatg gggcgattga ttaatcgcac
    12061 cctgacgcgc cacagcgagt taagtttcag cacctttttt gtctcttcga tgagcgagct
    12121 attaaagcag gttgccctcg acggctgtgg gattgcctgg ctgccggagt atgccatcca
    12181 acaagaaatt cgcagcgggc aactcgttgt gctgaatcgg gacgaactgg tgatcccgat
    12241 tcaggcctac gcataccgta tgaatacccg catgaatcca gttgccgaac gcttctggcg
    12301 tgaactgcgc gagctggaga ttgtgcttag ctgacattct caagcgtcgc actgcgccaa
    12361 ccgccattat cccagaccag atcaaacgtt agcggttcat cgtcgtggta cagcattatc
    12421 tcaaggctga tcgccagagt gtggcagtac gcttctttca aattaacctg caacgccgca
    12481 atccgtggct catgaaataa cagtgcagaa cgcagctgac ggcaatacca gctctggaga
    12541 tgtttgtcgc tcgggttatg cacttgccac tcgggcatgc ctgaaacaag taatgacggt
    12601 aatgccgaat gaggagaatt aaaaagagtg aggagatcgc tgcataccga tttacgcagc
    12661 aatgacgttt tattgcctga aatttcaggt aaaacagcct gaagtgattg gcgcatcatt
    12721 ttgcgcactc ctgacgttta gcaacatatg cggcttgccc tgaacaccgg gcaccactgt
    12781 attgatgctc attttccata gccattccct tttgttcatt acatccatgt cagtctgaga
    12841 caggcctgaa tcctgtcagc aaagtataaa accttacttg cctatgaata tctactgact
    12901 tttcaggaaa aaagtcatga tttgataaaa atcatcaaac tcaaaaaaaa accgctagta
    12961 tattctgaat attccgaata gttacgtaga tgaaaatact tgtcaccgcc gctacttaat
    13021 tccggtggga aattatttat ttccgcataa tcagccatgt attatttgaa acaattagtt
    13081 tgtaaataat ataaattga
//
LOCUS       DAJTNA010000110        13069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTNA010000110 DAJTNA010000000
VERSION     DAJTNA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13069
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.111"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..893
                     /locus_tag="OO161_002677"
     CDS             <1..893
                     /locus_tag="OO161_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCR5463697.1"
                     /translation="GRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPGRQ
                     AYRELRLLAGQAARQQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVPRK
                     YHLIRTAYEINEEPTAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAAD
                     QGACAPWTRGNNCPLAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKERRE
                     LAARLRLVKPKRRKDYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAG
                     LRIFYRNQRLQEDDQWRNLY"
     gene            1047..1235
                     /locus_tag="OO161_002678"
     CDS             1047..1235
                     /locus_tag="OO161_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463698.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            1246..1479
                     /locus_tag="OO161_002679"
     CDS             1246..1479
                     /locus_tag="OO161_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCR5463699.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            1772..3421
                     /locus_tag="OO161_002680"
     CDS             1772..3421
                     /locus_tag="OO161_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5463700.1"
                     /translation="MLVKKNKNYSLYFLITNNQQISDSGNYIKIVPSRSDWNDFGYLS
                     RVEISIFNKKECKDIISLNGYIGFLNSEEERNGKAELIRRVKKKEESNLKDEGFNDYF
                     VMLQDMNNYRRLVRFFGVNESKYILNFINDVVSKKSDASAENLRKKAISSDIFNKSFI
                     RESESYFTFKNAGTVLSGIQYEELGNLSQHIKISYSNKVNEEDVSYVFNFDHEHDLPK
                     RISIIIGENGVGKSQTLREIALAAIKGKDNLVAVVDSNGVSIEERIQISRLIAFSPTN
                     ESKCSFPTDKRIKSLIWYKRLTLNRESKSKLNLNDLIVQLARSNDAIGYNQRWNIFVD
                     ALSVLNSNNDLVIKCGGENSPFVKLNELNSRGEESRLSLYSEIILSGEPLRLIENKCY
                     PLSSGEISFIKFCVQVCLYIENGTLLLIDEPETHLHPSFINKFMALLDNLLEKTGSSA
                     IIATHSVYLVREVFKEQVTILRRRDDGVIVSEKPRLSTFGANIGNISYFVFGESEPTQ
                     ILNKVKNNIIAKNMSWDDVESKYSEDLSLNVLTALRNEMGN"
     gene            3405..4286
                     /locus_tag="OO161_002681"
     CDS             3405..4286
                     /locus_tag="OO161_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463701.1"
                     /translation="MKWAIEMNKLKRPVFKDYSAFKKLSANKRLKHHSVLNGQDTFIL
                     NAYRSYIKNKGYLNVNSCGVLSNNIAEALRYYYNNPAKCLSIIDDIRAANSNSLCPMC
                     GSMHSGTLDHVLPKENYPEFSLFTKNLVPACKCNTFKSTTIANSAGHRMLHPYFDNIL
                     KQRLICANFSKLGRTPTIDVKIIIDPMSPEYNNVKYHLDNVVIKNNIKGYLSEQWESF
                     YLYPELVIRGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIRPDVIRW
                     ILCELHLGNRSPDGRLI"
     gene            complement(4394..5425)
                     /locus_tag="OO161_002682"
                     /pseudo
     CDS             complement(4394..5425)
                     /locus_tag="OO161_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520363.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(5425..7191)
                     /locus_tag="OO161_002683"
     CDS             complement(5425..7191)
                     /locus_tag="OO161_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HCR5463702.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVYSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKDIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDDQIEKLEKNFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQAYAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            7334..8167
                     /locus_tag="OO161_002684"
     CDS             7334..8167
                     /locus_tag="OO161_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCR5463703.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            8184..9242
                     /locus_tag="OO161_002685"
     CDS             8184..9242
                     /locus_tag="OO161_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HCR5463704.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQISDFSAAAAETGA"
     gene            9246..9896
                     /locus_tag="OO161_002686"
     CDS             9246..9896
                     /locus_tag="OO161_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HCR5463705.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IWRHALRHGWVMPLGNRNVQTVLTEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            9992..10438
                     /locus_tag="OO161_002687"
                     /pseudo
     CDS             9992..10438
                     /locus_tag="OO161_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
     gene            10438..10641
                     /locus_tag="OO161_002688"
     CDS             10438..10641
                     /locus_tag="OO161_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCR5463706.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFVP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            10645..10860
                     /locus_tag="OO161_002689"
     CDS             10645..10860
                     /locus_tag="OO161_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463707.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            10841..11353
                     /locus_tag="OO161_002690"
     CDS             10841..11353
                     /locus_tag="OO161_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5463708.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            11355..11732
                     /locus_tag="OO161_002691"
     CDS             11355..11732
                     /locus_tag="OO161_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463709.1"
                     /translation="MKKKLISGLFLMLWMALLIAAIVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALASLIKNGPLWQELRASLLKTITRKENVFISWVIRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            11729..12157
                     /locus_tag="OO161_002692"
     CDS             11729..12157
                     /locus_tag="OO161_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HCR5463710.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSVLLRQRQRRIEELKRENEDLRRWADT
                     PLPADIIRLRERPALTGGAAYRQWLSASDAVSAGAGSAAH"
     gene            12253..12684
                     /locus_tag="OO161_002693"
     CDS             12253..12684
                     /locus_tag="OO161_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5463711.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVASGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSNVEAVAEPDEPEEMWTVKRG"
     gene            12677..>13069
                     /locus_tag="OO161_002694"
     CDS             12677..>13069
                     /locus_tag="OO161_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR5463712.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFTKLRTTKYLKTAASADSASVQFEGRVQRIA
                     RIHHYGLSDRVSRKGIYTIYAKRQLLGFN"
BASE COUNT         3356 a         2985 c         3542 g         3186 t
ORIGIN      
        1 acgggcgtgg tctggctggc gagatcagca aggaaacggg taaatccctg cgtgataacg
       61 ctgaatacgt taatccctgg gcgtctctgc atcgtgttca gcaattccgc ttctttggta
      121 ttccagggcg tcaggcttac cgtgaactgc ggttgttggc tggtcaggcg gcaaggcagc
      181 agggtgacaa aaaagcaggt gcgccggtac tggataaccc gcgccttgat gccatcctgg
      241 ctgctgctga tgctggctgt tttgccacct acattatgag gcagggcggc gtgctggttc
      301 ctcgcaaata tcacttgatc agaaccgctt atgaaatcaa tgaagagccg accgcctatg
      361 gcgatcacgg tattcgtatt tatggcatct ggtcacccat tgcagaaggc aagatctgca
      421 ctcatgcagt gaagtggaaa atggttcgta aagccgttga cgttcaggag gcggcagccg
      481 accagggcgc ttgcgcccct tggactcgtg gcaataactg tccccttgct gaaaatttga
      541 accaacagga gaaagataaa tcagctgatg ggaaccccag aacggacatt accagcatgg
      601 atgacaagga gttgcacgat tacctgcaca gtatgagcaa aaaagagcgc cgggaactgg
      661 cagcaaggtt acgcctggtg aaaccgaaac ggcgtaaaga ctacaaacag cgaattacag
      721 accatcagcg actgcagctc gtgtatgagc tgaagtccag aggatttgat ggcagcgaga
      781 aagaggtcga tttactcctt cgcggaggca gtattccgtc aggagcaggc ctgcgtatct
      841 tctatcggaa ccagcgtttg caggaagatg atcagtggcg gaacctgtat taattacgcg
      901 ggttaacaat tcgtgctctt aataatacca ggcatatcag gctgatgaac gtaaaaaaac
      961 gttttacatc agtaagatta ttatatactg taaatataaa cagtggttat gcatgcagta
     1021 ttgcgtgtgg tgtcatagga ggaaagatgc aggactattt tttggagtct ttgaagctcc
     1081 agcgcattga tttttttctt aagcttgtag cggctagtga gtgtagtgat gaagagaagg
     1141 ggctggctct gcagtgggtt tctgaattga ctgatgaact catggcaaaa atcagaaccc
     1201 acgaatacaa ccgctcaatg gatgtcatca gctgaggtga cttttatgcg cattgaaata
     1261 atgatcgata aagagcagaa gattagccag tctaccctgg acgctcttga atccgagctt
     1321 taccgcaatc tgcgccctct gtatcccaaa acggtaattc gtatccgcaa aggtagctct
     1381 aacggtgtgg aacttaccgg attgcaactg gacgaagaaa gaaaacaagt gatgaaaatt
     1441 atgcagaagg tgtgggaaga cgacagctgg ctgcattaag aaacgttgct ggcgtctgaa
     1501 ctagcttctg gcgtcagcaa ggttgaacaa cgagcccttg cgaggcgtta gctctgtagt
     1561 gcatgtctat gccgcatgag atcgcatgat cgtttgagga tcgtttttgc taaggcccgc
     1621 cagaactggc gggcttttgc gtagatcatg caggtgcatg aaaaccacta cataaagcgg
     1681 gcaggcgtgg cggggatacg attgcgcgct aggtataagt gtgtaaaata attccaaaat
     1741 aaatcatcac taatgaagtg agagggtaat aatgttagtc aagaaaaata aaaattatag
     1801 tttatatttt ttaattacaa ataatcaaca gatatctgat tctggtaatt atataaaaat
     1861 agtcccatca agaagcgatt ggaatgattt tggatatttg agtagagtcg aaatatctat
     1921 ttttaataaa aaagagtgta aggatataat ttcactgaat ggttatattg gttttttgaa
     1981 ttcagaggag gaacgaaatg gaaaagcaga actgataagg cgagtgaaaa aaaaggaaga
     2041 atctaattta aaggatgagg ggtttaatga ttattttgtc atgcttcagg atatgaataa
     2101 ttatcgaaga ctagtaagat tttttggggt gaatgaatcc aaatatatat taaatttcat
     2161 taatgatgtt gtttctaaga aaagtgacgc tagtgcagag aatttgagga aaaaagcaat
     2221 ctcttctgat atctttaata aatctttcat tagagagtct gagtcgtatt ttactttcaa
     2281 aaatgcagga acagttcttt ccggtattca atatgaggag ttgggtaatt tatcacagca
     2341 catcaagatt agctattcga ataaagttaa tgaagaagat gtatcttatg tttttaattt
     2401 tgatcacgag catgatttac caaaaagaat ttccataatt ataggcgaga atggtgtagg
     2461 aaaaagccaa acgcttagag aaatcgcatt ggctgcgatt aaaggaaagg ataatttggt
     2521 ggctgtagtt gattctaacg gtgtgagcat tgaagaacga attcaaatta gtaggctcat
     2581 tgctttttct ccaacaaatg aaagtaagtg ttcatttcca accgataaaa gaataaaatc
     2641 attgatatgg tacaaaagat tgactttaaa tcgtgaatca aaatccaaat taaacttgaa
     2701 tgatttaata gtgcaactag cgaggagtaa cgacgctatc ggctataatc aaaggtggaa
     2761 tatatttgtt gatgcactga gtgttctaaa tagtaacaac gatttagtaa tcaaatgtgg
     2821 cggtgaaaat tcaccatttg ttaaattaaa tgagcttaat tctcgaggcg aagaatctag
     2881 actatcatta tattctgaaa ttattctctc tggcgagcct ttgaggctta ttgaaaataa
     2941 atgttaccct ttgagtagtg gggaaatatc gtttattaag ttttgtgtac aagtttgttt
     3001 gtatattgag aatgggactc ttctattaat agatgagcca gaaacacatc tccatccttc
     3061 atttattaat aaatttatgg cattgcttga taatttatta gaaaagacag gctcttcagc
     3121 tatcatagca acgcattcgg tttatcttgt tcgggaggtt tttaaggagc aagtgacaat
     3181 actcagaaga cgagatgatg gggtaattgt tagcgaaaaa ccgagattga gtacttttgg
     3241 ggcgaatata ggaaatatct catattttgt tttcggtgag agtgaaccaa ctcaaatttt
     3301 aaataaagta aaaaacaata taattgcaaa gaatatgtct tgggatgatg ttgaatctaa
     3361 atatagcgag gacttatcac ttaatgtatt aactgcgttg agaaatgaaa tgggcaattg
     3421 aaatgaataa attaaaaaga cctgttttta aagattattc tgcatttaaa aaactatctg
     3481 caaataagag attaaaacat cattctgttc tgaatggaca ggatacattt atacttaatg
     3541 catatagatc gtacataaag aataaagggt atcttaatgt caattcttgt ggcgtattga
     3601 gtaacaatat tgctgaagct ttgagatatt attataacaa tcccgcaaag tgtttatcta
     3661 taattgatga tataagagca gctaattcta actctctatg tccaatgtgt ggttcaatgc
     3721 atagcgggac tttggatcat gttttaccca aggaaaacta tccagaattt tctttattca
     3781 ctaagaattt ggttcctgct tgcaaatgta atactttcaa aagtactact atagctaatt
     3841 ctgcaggcca tcggatgctg catccatatt ttgataatat attaaaacaa agattaatat
     3901 gtgctaattt ttctaaatta gggcgaacac ctacaattga tgtaaaaatc ataattgacc
     3961 caatgtcacc agaatataac aatgttaagt atcatcttga taatgttgtt atcaagaata
     4021 atattaaggg gtatttgtct gaacaatggg agtcgtttta cttatatcct gagcttgtta
     4081 ttagaggact gaataagagt ctttctactt atagcgatgt atatgagttg ttgactcgcg
     4141 agttgttgct gttggatgaa aaacataaag gtaaaaataa ttggaattcg gtttttgtag
     4201 ctgggttaat tcgacctgat gtgattaggt ggatactctg tgaattacat ttaggtaaca
     4261 gaagtccaga cggtcgtctc atatgatttt ttgcatcttc agcgtgtatc tgcacgctga
     4321 agaaattcat aaaaactgct gcctattaat aaattttttt agctgagtta aagaacctca
     4381 tttagtgatt acgttaagta gctagtttat aactatcaaa tttaacaatg ttctcactta
     4441 ccaatttatt tatttccatc attctttttt gtaacggtat taactcgtta cgtacaaaca
     4501 ccaggctcgc cttctccaca tccccaaacc ccccgacatt attaggcata atccccatca
     4561 tttgcggtgg cacacgatgc gccgccatca tgtcatcccg gctcacgttc ttgatgttca
     4621 gaaactcatc cttcgccgcg acttctgaca acgggatgat ctgaagcccg tcctttttgc
     4681 cgttaggcga gtacataaac agattgcgga agttacctgg tcctttggcg cttttcatcg
     4741 cattgcggag gttgttcaca tcctcctggt tttgcgcggc atcggtcatg tacatgatga
     4801 agcctgcatg actgccgtta atgtaatact atcggcggaa cagcgtggcg gactcgttga
     4861 gcagggctga cggaatggca gaaaggtaac ctggcaggcc gtagatctct tggttgatgt
     4921 ccggttccat cagatgaaag atgcttcctt tcgtgaactg atacggctgc gtggtcatgc
     4981 cgtattgcac aaaccagtag gtatccaggt ctaatccgcg tcgggtgtat tttgccagag
     5041 caggctcaag agcgatgacc tctccgaacc ggtttgtgcg tttctccagg taagcgttac
     5101 caaataccag ataatcctgc acaaaacgtg aaaaagcctg ctggctgagc agcgggtgag
     5161 ggatgtaggt actgatcaga atgttgcatt tcaccgcaat tggtgaactg tgatgcacgg
     5221 cggcgcggaa ggtgcgcgcc agtccgtcaa aactcactgg cggctcatac cagcgatctg
     5281 tctgtacgca ttccacatag tccagtagtt cacggcggtc cagaactgga acgggatcgc
     5341 cgaagctgaa tgcttcggct gaagtctggt ttttatgctg ggtctgtttc gtcgccgcag
     5401 cgcggttctt cttactcttt cccatcaaaa aatctccaca atattgctgg tattggcgga
     5461 ctcgccctgc agcggttcgt taaacagtgc gtgcattgtt gcccaggcca gatcggcatg
     5521 gctggcttct tcgctgcggc tggcttcata ggtcgggcgg ttgccgctgg cggtggtggc
     5581 gcgacggatt gccatgaatg actgcgcaat gtcggtgtgc ccggcgtcaa actcaagacg
     5641 gcggtggctg ataatgtcgt aggccttgag taccagggcg tttttaacgt tggggttgta
     5701 gacaaactcc cggacggcag gaaaaaacgc tttcacgttc tcgtaaaccc cgtgaccaac
     5761 gccggttgag tcgatgccga tataggtcac gttgtactgt tcagtcagtt ttttgatggc
     5821 gtaagcctgg gcgcgaaagt ccattccgcg ccactggtga cgctcaagaa tgcggaactt
     5881 accgcctggc acggctggcg gtgccaccac cacgcatccg gcgctgtcgc cgttctgcgt
     5941 accttttgcc gggtcataac cgatccacac ttcgcgccag ccaaacgggc gcagggccag
     6001 tgcatgaaag tcggtccaga cttcccagct gtccaccatg cacgcctgca attcgctgag
     6061 cgggaacacg gacgcgagat cgtccacgaa ctcgcacatc agcaggttct ggtattcgtc
     6121 agggctgtac tccatgcgca actggtcaag gtcgaacagg ttacaggcgc cgcgcaccgc
     6181 atcttccacg gtgactatct ggcggtattg cccgtctgcg cacagcaggc cgggggccag
     6241 attgctgtgg gacaggtcga tgtccacctt gtcagctttg ttgcgcccac ggttgaacag
     6301 cgcaccggac cagaacggat aagcactgtg ggtcagactg gatggtgtgg aaaaataggt
     6361 ctgccgccat ttcttgtgaa tagccatacc agaagccact ttgcgtagct cctgaaattt
     6421 cggtatccag aaatattcat ccagatacag gttaccgtga taactctggg cagtgcgggc
     6481 attggtgccg aggaaataca gtgtggcccc gttgggaagc accatcggat cgcctttcag
     6541 ctccacctcc acttctttgg caaagtcgat gatgtactgt ttgaagacgt gggcctgtgc
     6601 cttactggca gaaaggaaaa tctggttacg tccggtaagc agggcgtcaa tcagggcttc
     6661 acgggcaaaa tagaaggtcg cgccgatctg gcgtgacttc agcaggttgc ggatgcggtt
     6721 ggtttttccg gcttcccacc agtggcgctg gtagttgaac atggaggaat ggaaattttt
     6781 ttccagcttc tcaatctggt catcggtgaa aacattcttt tcaggctgac ggcgtgggcc
     6841 tttgttgcgg ttggcgacgt tagggtttaa gtcggcttcg ttgccgccat tgttaaactt
     6901 gccgatccgc gcgtggcgct cagactggcg cgccagcagg tcaatatctt tgaaatcttt
     6961 cccttctttg tgctccttca tgatgagctg gcagtagcgt gcggcggtgg tgagctgcat
     7021 ctgatccaga ggcccatagt caccccactt gtcgcgtttt ttccagctgt aaacggttgc
     7081 aactttctcg cccagcattt cagcaatgcg ggctacgcgg tatccctgaa agtacagcag
     7141 catggcctgc cgacggggat cgagatctgc gggtgtcagt gtggtgttca tggcacaaac
     7201 ctacagcctt gaatgaaggc tttccccgcc tgcggtttgt gtggttgtcg gtacaaatac
     7261 cgcgcattgt ttcactgccc ctatcaccgc aaccataagg ctccagtaag ttttttctaa
     7321 cggagcacgg ctcatgacag tgaaagcaaa gcgttttcgc atcggggtgg aaggtgccac
     7381 caccgacgga cgcgaaatcc agcgtgaatg gctggaacag atggcagcca gctataaccc
     7441 ggcggtgtat accgcgctga ttaaccttga gcacatcaag tcttatctgc cggacagcac
     7501 ctttaaccgc tacggcaagg tgacggcgct gtttgctgaa gaaatcacgg aaggtccgct
     7561 ggcaggcaag atggcgctgt atgctgacgt tgagccaacg gagtccctgg tggaactggt
     7621 gaaaaaaggc cagaaattat tcacctctat ggaagtcagc ccgaagttcg ctgatacggg
     7681 caaagcctac ctggtcggcc tggctgccac tgatgaccct gccagtctgg gcactgaaat
     7741 gctgacattc agcgccagtg cagcccataa cccgctggca aaccgcaagc agaatcccgc
     7801 caatctcttt accgccgcag aggaaacggt gatcgaactg gaagaagtcc aggacgacaa
     7861 accgtccctg tttgcccgcg tcacggcgct gtttaccaaa aaagagcagt ccgatgacgc
     7921 ccggttctct gatgtgcata aggccgtgga gctggtcgcc actgagcagc agaacctgag
     7981 cgcacgcacc gaaaaatccc tgtctgagca ggaagaacgc ctgtctgagc tggagactgc
     8041 cctgcaggca cagcaaaccg cctttaacga actggtggac aagctgagtc atgaagacag
     8101 ccgccaggac taccgccagc gtgcaacagg cggtaacgcc cccgctgaca ctctgaccaa
     8161 ttgctgatgg agcacaaaac ctgatgaaga agaatacccg ctttgctttt aacgcttacc
     8221 tgcagcaact ggcgcgtctg aacggtgtgg cagttgaaga actgtccagc aagttcaccg
     8281 tagagccgtc cgtgcagcag acgctggaag accagatcca gcagtccgcc gctttcctga
     8341 ctctgattaa cgtcacgcca gtgactgagc agtccggtca gctgctgggg ttgggagttg
     8401 gcagcaccat tgccggaacc actgatacca ctgcgaaaga gcgtgaacct gtcgatccga
     8461 cgctgatggt cgatgtggaa tataaatgcg agcagaccaa ctttgacacg gtgctgacct
     8521 acgcgaagct ggacctgtgg gcgaagtttc aggattttca ggtgcgtatc cgtgacgcca
     8581 tcgtgaaacg tcaggcactg gaccgcatca tgatcggctt taatggcgtg aagcgtgcga
     8641 aaacctccaa ccgtagcgaa aacccgctgc tgcaggatgt gaataaaggc tggctgcaga
     8701 aaatccgtga ggatgcaccg gatcacgtca tgggcagcac caccacgggc ggtgaaacca
     8761 caccgggcgc agtgaaagtc gggaaaggtg gcgaatatgc caacctggac gccgtggtga
     8821 tggatgccgt caatgagctt atcgacgtgg tctaccagga cgatgacgat ctggtggtga
     8881 tttgcggtcg tgaactgctg tctgacaagt atttcccgct ggtcaacaaa gagcaggaaa
     8941 acagtgaaaa actggctgcc gatatgatca tcagtcagaa acgcatgggt ggcctgcagg
     9001 ccgtgcgtgc gccgttcttc ccgccgaatg cgctgctgat cacccgtctg gataacctgt
     9061 ccatctactg gcaggaagac acccgccgcc gttcagttat cgacaacccg aaacgtgacc
     9121 ggattgaaaa ctttgaatcc gttaacgaag cctatgtggt tgaggactac cgctgcgccg
     9181 cactggtgga aaacatccag attagtgatt tcagcgccgc cgcagccgaa accggagcgt
     9241 aatccatgag cctgagtccc gcacggcagc atcgcctgcg cgttcaggct gaacaggccg
     9301 cccgtgaggg tggcagtgtt cgccacgcat cgggctatga cctgatgctg ctgcaactgg
     9361 cggaagaccg ccgccgtctc aagggcgttc agtccacggt caaaaaagcg gaaatcaaag
     9421 tggagctgct gccgaagtac gccgcctggg cggagggtgt cctggctgcc ggaggcgctc
     9481 aacaggatga cgtgctgatg tacgtgatgt tgtggcgcat tgatgccgga gattatgccg
     9541 gggcgctgga gatctggcgt catgccctgc gtcatggatg ggtgatgccg ctgggcaacc
     9601 gcaacgtgca gaccgtgctg acagaggaaa tggcagatgc agcgcagagc gcaatgcttg
     9661 ccgccaccgg ctttgatgcc gatctgttgc tgcagacgct ggagctgaca gacggtctgg
     9721 atatgccgga ccagtcacgg gcacgtctgc ataaagcgat tggcgcggtc ctgagtgaaa
     9781 gcaatccggc ttccgccctt aatcatctca accatgcgtt acagctcgat ccccgctgtg
     9841 gcgtgaaaaa agacaaacag cagctggagc gcagactgcg caatgacagc cgctgacaga
     9901 acgtgccccc gcgcacgggc ggcacggggt ggcgaaaggc acagccacat caaaaccccg
     9961 tccaccgccc tttatttcag gagaaagcag catgaagttt gttgcgccag aacaggcacc
    10021 ggaacaggcg gaaatcatta gaaatacgcc gttctggcct gatgtggacc tgtcggagtt
    10081 tcgcagtgtc atgcgcactg acggcacggt gacgcagccg cgtttaaagt aggttgcact
    10141 gtcggcaatt tcggaggtta acgcagagct gtatgaattt cgcagacgcc agcagatgct
    10201 ggggtatgcg tcggaacagc tggacgggaa aagtgagcgc attcagcact atttcaacgc
    10261 ggtttactgc tgggctcgcg ccatgctcaa cgaacgtttc caggactatg acgccacggc
    10321 atccggtgcg aagcggggcg aagaactggc ggaagcaagc ggtgatttgt ggcgtgacgc
    10381 ccgctgggcc atcagccggg tgcaggacgc gccgcactgc acagtggagc ttatctgatg
    10441 aaagtgcgtg cgcatcagta tgacacggtg gacgcgcttt gctggcgtca ttacgggcgc
    10501 acgcagggtg tcacggagca ggtactgaag gcaaatccgg ggcttgccga atacggcccc
    10561 tttgtacctc acgggctgca ggtggagctg ccggacattc cgaccaccac caccgtgcag
    10621 accgtccagc tatgggactg aattatgacg cttgagcgaa tcagcgcctt tatcacgtat
    10681 tgcatcgccg tcgtgctggc ctggctgggc gatttgtcca tcaaggatgc ctcaacgctg
    10741 ggcggcctga tgattggtgt gctgatgctg gctatcaact ggtactacaa acacaaagcc
    10801 taccagcttc tgcgcgacgg gcagatctcg cgggaggact atgaatccat caatcgttaa
    10861 acgctgcctt gtcggggccg tgctggctat tgctgccacg ctgccgggtt ttcagcagct
    10921 tcacacctcc gtggagggac tgaaactgat tgccgattac gaaggttgtc gtctgcagcc
    10981 gtatcagtgc agcgcgggtg tctggaccga cggtattggt aatacgtcgg gcgtcattcc
    11041 cggcaaaacc attacggagc gacaggcagc agaagggctg atctccaacg tgctgcgtgt
    11101 ggagcgggcg cttgaaaggt gtgtgaagca acagccgccg cagaaggtgt atgacgctgc
    11161 ggtgtcgttt gccttcaacg tgggtacggg caatgcctgc agttccacgc tggtgaaatt
    11221 gctcaatcag cggcgctggg cggatgcgtg ccgacagttg ccgcgctggg tttatgtgaa
    11281 aggtgttttt aatcaggggc tggataaccg ccgtgcgcgg gagatggcct ggtgtttaca
    11341 gggagcaaac tgaaatgaaa aagaaattaa tcagcgggct gtttctgatg ttatggatgg
    11401 cgctgttaat cgcagcaata gtgtatccgc aggggatttt tccggtactg gcagcgtccg
    11461 gcgtttgggt agcctgttta ctgacatggg cggtaattcc ggtagcactg gcttcgttaa
    11521 ttaagaatgg cccgctctgg caggagttga gggcatcttt actgaagaca attacccgaa
    11581 aagaaaacgt atttatcagc tgggtgatac gattgctgat tgtcgtcagt ctcgcctgga
    11641 cggggtgggc cattaccctg gtcttttatc tgctgaccgt tattgccttc tggatcaccc
    11701 gtaatcagat ggcgcaacag gtagccgcat gaaccggttg ctgctggttg tgctggcgtt
    11761 attattggcg gcgctgggct ggcagacgtg gcggctggct gatgccagcc agaccatcag
    11821 cacgcaggca gacgagctgc agagcaaaag ccaggcactg gcaaagagca acagccagct
    11881 tatcagcctg tccattctga ctgaaaccaa taaccgggag caggcgcggc tctatgccga
    11941 agcagaacag accagtgtac tgctgagaca acgacaacgc cggattgagg aactgaaacg
    12001 tgagaacgag gatttacgtc gctgggctga tactcctttg cctgctgaca ttatccggct
    12061 gcgggaacgt ccggcactca ccggaggtgc agcttaccgt cagtggttgt ccgcgagtga
    12121 cgccgtgtcg gctggagcag gcagcgccgc gcactaacgg tgatctgaat gcgttgctgg
    12181 atgaaacgga ggccgcctgg gcggtctgtg cagacaaagt ggacatgatt attgcgtgtc
    12241 aggagcgaaa tagtgaacaa accacaatcc ctgcgccacg ccctcaataa agcagtgcct
    12301 tatgtccgta ataacccgga caaactgcat ctgtttgtgg ataacggttc gctggttgcc
    12361 tccggggcta gctccatgtc atgggagtac cgctacaccc tgaacgtggt gattgaagat
    12421 ttcagcggcg accagaatct gctgatggcc ccggttttgc tgtggctgcg ggataaccag
    12481 cccgatgcga tcaataaccc ggcgttacgg gaaaagctat tcacctttga ggtggatatt
    12541 ctgcgcaacg atgtctgtga tatcagcctt aacctgcaac tgacggagcg tgtgctggtc
    12601 agcactgacg gcagtgtgtc gaacgttgaa gcggtagcgg aacctgatga acctgaagaa
    12661 atgtggacgg tgaaacgtgg ctgaattgca gaaggtggac gactggctga gtgcattatt
    12721 ggcgaatctg gagccagccg caagaagccg catgatgcgc cagctggcgc aggaactgcg
    12781 ccggacacag cagcagaata tcaggatgca gcgcaatcca gatggcagca gttatgagcc
    12841 gcgcagggta acagcacgca gcaaaaaggg gcgcatcaaa cgtcagatgt ttacaaagct
    12901 gcgcaccaca aaatacctga aaactgcagc cagcgccgat tctgccagcg tacagtttga
    12961 aggtagggtg cagcgcattg cccgtattca ccattacggc ttgagtgatc gcgtcagtcg
    13021 aaaaggtatt tataccatct atgcgaaaag gcagttatta ggttttaat
//
LOCUS       DAJTNA010000111        12873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTNA010000111 DAJTNA010000000
VERSION     DAJTNA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.112"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..383)
                     /locus_tag="OO161_002695"
     CDS             complement(<1..383)
                     /locus_tag="OO161_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463713.1"
                     /translation="MNFKKKLEEHFKQFEASPVLFVGSGVSRRYLGVPCWQDLLKHFA
                     EAIGENHIKLKTKSNGDLPEYAQLLVSAYAEKWWDTEEGQLALSEKEQEKTFINEQSP
                     LKLSISKYIENAHKNIIDNDELKHE"
     gene            complement(774..2042)
                     /locus_tag="OO161_002696"
     CDS             complement(774..2042)
                     /locus_tag="OO161_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5463714.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQRRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     AKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANREKGITPFDYAKINRGNGE"
     gene            complement(2243..2327)
                     /locus_tag="OO161_002697"
     tRNA            complement(2243..2327)
                     /locus_tag="OO161_002697"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2291..2293),aa:Leu,seq:caa)
     gene            2523..3542
                     /gene="ahr"
                     /locus_tag="OO161_002698"
     CDS             2523..3542
                     /gene="ahr"
                     /locus_tag="OO161_002698"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5463715.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDHSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            3672..5174
                     /locus_tag="OO161_002699"
     CDS             3672..5174
                     /locus_tag="OO161_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5463716.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5335..6417)
                     /gene="lptG"
                     /locus_tag="OO161_002700"
     CDS             complement(5335..6417)
                     /gene="lptG"
                     /locus_tag="OO161_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5463717.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQMKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6417..7517)
                     /gene="lptF"
                     /locus_tag="OO161_002701"
     CDS             complement(6417..7517)
                     /gene="lptF"
                     /locus_tag="OO161_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5463718.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7784..9295
                     /gene="pepA"
                     /locus_tag="OO161_002702"
     CDS             7784..9295
                     /gene="pepA"
                     /locus_tag="OO161_002702"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5463719.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9455..9898
                     /gene="holC"
                     /locus_tag="OO161_002703"
     CDS             9455..9898
                     /gene="holC"
                     /locus_tag="OO161_002703"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5463720.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9898..12753
                     /gene="valS"
                     /locus_tag="OO161_002704"
     CDS             9898..12753
                     /gene="valS"
                     /locus_tag="OO161_002704"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5463721.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAIIAKEREKLEGYAEAKAKLIEQQAVIAAL"
BASE COUNT         3152 a         3317 c         3424 g         2980 t
ORIGIN      
        1 atttcatgtt ttaactcatc attatctata atatttttat gtgcattctc aatatattta
       61 gatattgaca acttcaaagg agactgttcg ttaatgaaag ttttctcctg ttctttttca
      121 cttaatgcta attgtccttc ttcagtatcc caccatttct cagcatatgc agacaccaat
      181 aattgggcat attcgggtaa gtcaccatta gattttgttt ttaattttat atgattttcc
      241 ccaattgcct cagcaaaatg cttcaaaagg tcttgccaac atggcacacc aagataacga
      301 cgagacacac cggagccgac aaataacact ggggatgctt cgaactgctt aaaatgctct
      361 tcgagttttt tcttaaaatt cacacggata ctctctttaa aagaaatcaa gcccctttat
      421 actgattaca ttaagatatt gcggcttaca ccaccagtaa attggtcaat tttacgtaag
      481 aaatttaatt atttagattc aattaattat tagattaact aattgactat cactcctgat
      541 gtagccgaat tgctgtacct ttgttacgat gtagggctca atcaagggtc cactcccctt
      601 ttataagtaa tcatgacttt aaatttaggc ttaggtggag cattactgcc gattaggttc
      661 aataaaaagg cttaccttag tgaaaactcg gctatttggc actgctgacc aattcctcgt
      721 ttgaataagt cagcagcttt caatttagag gcgaatgtgc gcttttagat aactcactcg
      781 ccattacccc gattgatctt cgcatagtca aacggcgtaa tccccttctc ccggttcgca
      841 tccagaaaat cagcccacca ctgcaacatc agcttgcgct catccagatg ttccgcttta
      901 tggatatacg ccgccctcac cgaattgcgt tccatatggc tcatctgcct ttccaccgca
      961 tccttcgacc ataaccctga ctcaatcaat gagctacagg ccatagtgcg gaagccgtgg
     1021 ccgcaaacct caaccttggt gtcatagccc atcacccgca atgcactgtt caccgtgttt
     1081 tcactcatgg gcttactcgg atcgtgatcg ccaataaaaa tcagctcatg ttcgccacag
     1141 aactgtttta tctgcttcag gatcgctagc gcctgctttg aaagcggcac cagatgaggt
     1201 gtacgcatct ttgctcctcg ctgagaatgt ttcacaccgg gaataggttc tcgctcaggc
     1261 gggatggtcc acattgacgt ttcaaaatcg atttctgacc agcgagcaaa acgcagctca
     1321 ctggatcgaa taaaaataag cagggtaagt tcggttgccc agcgggttag tggcctgccg
     1381 gtgtattcat cgattttctg gagtaattcc gggatacgct ttaactccag cgccggacga
     1441 cgctgtcgat tactggaagc taccgccccc gccatctctt gcgccggatt gtaatcaatt
     1501 aacccgcttt gcactgcata acgcatgatg gccgtggtgc gctgttgaag gcgggaagcc
     1561 acttcaagac gtccggattt ctccacggct ttaataggaa ttaacagatc gcgggtgccc
     1621 agttcagcaa tattgtggga accaattgcc gggaagatat tgtcctccag gctctttttt
     1681 acacgattag catgctcttc cgaccatttt tggttggtta cgagccactc tctggcaacc
     1741 ttctcgaaag taataatctc tttcgcctgc tcttctttca cagcacgctt atgttcacga
     1801 ggatcgatac cagcagctac cagctttctg gcctcttccc ttttcttccg cgcatccgcc
     1861 agcgaaacat ccgggtaaac gccgaacgcc atcaaatgtt gtttaccgcc aaaacggaaa
     1921 cgaaatcgcc aatactttga accgccaggt tttacaagta aggacatgcc gtcgccgtca
     1981 acaagtgaat actctttctc ctcaggtttc gcagagcgaa ctttagtatc cgttagggcc
     2041 ataatggtct tccttccatt ggtatctcgt gagtatacaa gcaatatcga atcggcaaat
     2101 atactcatta gtatactcac aagcctgttg atgtgggttg agtcaggttg acttcagttg
     2161 aggggaaaag ctggaaaagc cttgtgtagc gcggatttca ggcataaaaa aagacctcag
     2221 ttgaggtcta tttacatact tttggtgccg aaggccggac tcgaaccggc acgtatttct
     2281 acggttgatt ttgaatcaac tgcgtctacc gatttcgcca cttcggcact gaagaggtat
     2341 gcggaaaacg ttgtggatta tacctgtcgc acgccaccat gcaagcgcca gcttgctcgc
     2401 acctcgctaa gtgctgaaat tttcagcatt gcacacagcg atgtgtaacc tttgtcacac
     2461 tccaggcacc ccgccctgcc atgctctaca cttcccaaac aacaccagag aaggaccaaa
     2521 aaatgtcgat gataaaaagc tatgccgcaa aagaagcggg cggcgaactg gaagtttatg
     2581 agtacgatcc cggtgagctg aggccacaag atgttgaagt gcaggtggat tattgcggga
     2641 tctgccattc cgatctatcg atgatcgaca atgaatgggg attttcacaa tatccgctgg
     2701 ttgccgggca tgaggtgatt gggcgcgtgg tggcactcgg gagcgccgcg caggataaag
     2761 gtttgcaggt cggtcagcgt gtcgggattg gctggacggc gcgtagctgt ggtcactgcg
     2821 acgcctgtat tagcggtaat cagatcaact gcgagcaagg tgcggtgccg acgattatga
     2881 atcgcggtgg ctttgccgag aagttgcgtg cggactggca atgggtgatt ccactgccag
     2941 aaaatattga tatcgagtcc gccgggccgc tgttgtgcgg cggtatcacg gtctttaaac
     3001 cactgttgat gcaccatatc actgctacca gccgcgttgg ggtaattggt attggcgggc
     3061 tggggcatat cgctataaaa cttctgcacg caatgggatg cgaggtgacg gcctttagtt
     3121 ctaatccggc gaaagagcag gaagtactgg cgatgggtgc cgataaagtg gtgaatagcc
     3181 gcgatccgca ggcactgaaa gccctgtcgg ggcagtttga tctcattatc aatactgtga
     3241 acgtcagcct cgactggcag ccttattttg aggcgctgac ctatggcggt aatttccata
     3301 cggtcggtgc ggttctcacg ccgctgtctg ttccggcctt tacgttaatt gcgggcgacc
     3361 acagcatctc tggttctgct accggcacgc cttatgagct gcgaaagctg atgcgctttg
     3421 ccgcccgtag caaggttgcg ccgaccaccg aactgttccc gatgtcgaaa attaacgatg
     3481 ccatcaagca tgtgcgcgac ggtaaggcgc gttaccgcgt ggttctgaaa gccgacttct
     3541 gatccctttg ccgggtgcct gtcgcccggc acttaaatct ctttggaagg cgtctcgcaa
     3601 tctgctgaat ttagcggtga agagtgcgca gccgttgcct atactccagg caggaaactg
     3661 gaggaaatct catgagcgaa cccctattaa ttgcccgcac gccggacaca gaactttttt
     3721 tactgccggg aatggctaac cgtcacgggc tgattactgg cgcaacgggg acgggtaaaa
     3781 ccgttacgct gcaaaaactg gcagagtcat tgtcggaaat cggcgtgccg gtgtttatgg
     3841 ctgatgtgaa aggcgatctg accggtatcg cgcaggcagg aacggcgtcg gaaaaactgc
     3901 tcacaaggct taaaaatatc ggcgtcaatg actggcaacc gcatgctaat ccggtggtgg
     3961 tgtgggatat ctttggcgag aaaggccatc cggtgcgggc gacggtttca gacctggggc
     4021 cgctgttgct ggcgcggctg ttgaatctca acgatgtgca atctggcgtg ctgaatatca
     4081 tcttccgtat tgctgacgat cagggattgt tgctgctcga ctttaaagat ctgcgggcga
     4141 ttacccagta catcggcgat aacgccaaat ctttccagaa tcagtacggt aatatcagta
     4201 gcgcatcggt tggtgccatc cagcgcggtt tactgtcgct ggaaaagcaa ggtgcggcgc
     4261 atttctttgg cgagccgatg ctggatatca aagactggat gcgcaccgat accaacggta
     4321 aaggcgttat caatatcctc agcgccgaga agctttatca gatgccgaaa ctgtacgccg
     4381 ccagcctgct gtggatgctt tcagagttgt atgaacaatt gccggaagcg ggcgatctgg
     4441 agaaaccaaa actggtgttt ttcttcgacg aggcacatct gctgtttaac gatgcaccgc
     4501 aggtactgct ggataagatt gagcaggtaa taaggcttat tcgctcaaaa ggcgtgggcg
     4561 tctggttcgt ttcgcaaaac ccgtctgata ttccggataa tgtgctcggg cagctcggta
     4621 atcgcgttca acacgctttg cgggctttta cgcccaaaga tcagaaagcg gtaaaagctg
     4681 cggcgcaaac catgcgggcc aatccggcgt ttgataccga aaaggcaatc caggaactgg
     4741 ggaccggcga ggcgttaatc tcgtttctcg atgcaaaagg aagtccttct gtggtggaac
     4801 gggcgatggt gattgcgcct tgttcgcgga tgggaccggt gacggaagat gagcgcaatg
     4861 gcttgattaa ccactccccg gtgtatggca agtacgagga tgacgtggac cgggaatccg
     4921 cctatgagat gttgcaaaaa ggctttcagg ccagtaccaa gcagcaaaat aatcctcccg
     4981 cgaaagggaa agcggtggcg gtggatgacg gtattcttgg tggattgaag gatattttgt
     5041 ttggcactac cggaccacgc ggcgggaaga aagatggtgt ggtgcaaaca atggcgaaaa
     5101 gcgccgcccg ccaggtgacg aatcagattg tacgtgggat gttggggagt ttgctggggg
     5161 gaagaagaag gtaagtcagg cgcgatttgt ctttgcctga tgcgacgctg gcgcgtctta
     5221 tcatgcctac accactgcaa tatattggat tttcatgatt tttcaggcat gaacgaagca
     5281 cactttgcta acaataataa agggagcttt cgctcccttt attcgttcat tcggttacga
     5341 ttttctcatt aacagccaca ggctgattaa gaagaagctg gcgcttggca acagtgcgcc
     5401 gatgatcggc gggatgccat aaaccaacgt cagcgggccg aagatctggt ccagtacgta
     5461 gaagacaaaa ccgaagctga taccggtgac cacacgcacg cccatcggta cgctacgcag
     5521 tgggccaaag atgaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa
     5581 gattttgctc cacatattga gctgataacg tccggcatcc tgaccgctcg acttcagata
     5641 cttcacgtag ttgtgcaaac cgctgattga gagtgcatcc gggtccagcg ccaccacgcc
     5701 tagtttgtcc ggcgtgaggt tggttttcca ggtgccgctc accgtctgcg aaccggtgat
     5761 ctgtttcgga ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc
     5821 cgggtcaaac ttcgcagtag cggcatagcg tacggattgc agacgacgat tctcgttaaa
     5881 ggcataaata ctgatgccac ctaactcttc gtcaccttta acccgctcaa tgtaaacgaa
     5941 gttgttgcca tctttcgccc ataagccttg ctgggtagag agcaacgagc cgccgtacat
     6001 cgcctgcgca cggtagttac gcgccatctg ctcgccctgc ggcgcgaccc attcaccaat
     6061 cgccatcgtc agcaagacca gcggaatggc ggttttcatc accgacagcg caacctgcat
     6121 acgggtgaaa ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag
     6181 accaagcaac gccccaagca gagccgccat cgggaagaat gtttgcacat ctttcggcac
     6241 gctcagcaag gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt
     6301 catctgatcg acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat
     6361 gatggtggtg aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac
     6421 accgctcctt tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg
     6481 ttgagaacaa tcgccaaagc cagataaatc aggttaacgg tccacatcca cagcgtcggg
     6541 tccagcttac ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga
     6601 tacagcagca tggctggcag catcgatagt acgcgtccct gacgcgggtt aaccacgctc
     6661 agcggtacga ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc
     6721 agttctgcgc gggcacgatc ggtgtcagtg ttccacaatg tacgcatgtc catctggtcg
     6781 gtatcgttcg ggtcgagcgc taccgcctgg tgaccaatga tcgcctgata atcctggaag
     6841 tccgtaatgc ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga
     6901 gtgacgacct gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca
     6961 gaaggacgtg cattaccttt tggtcgaatt tgcgcgagga acacatcttt gaaatcgctg
     7021 ccgtcaacgc tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct
     7081 tgcgccagcg ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga
     7141 cgcgatgacc acggtcccgc ccacatcacg ttaaccgccg cgacgattgc agtgaatacc
     7201 gcaaggatca ttgccgcttt caccagaacc gctttgctca ggccgcaggc atgcattacc
     7261 gtaatttcac tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt
     7321 aatggcagga taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga
     7381 ttcgccggaa tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag
     7441 aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga
     7501 tatcttatga ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc
     7561 ttgtttcatg gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa
     7621 ttatactcaa cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt
     7681 taagattaac acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat
     7741 ctgtagccac cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa
     7801 aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg
     7861 tcgcctttct ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct
     7921 gctacgtcgg ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc
     7981 gaatgtactt tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga
     8041 gcgtcagtac aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcaat
     8101 ggaagcggtc tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt
     8161 gcgtcaggct gtcgagacgg caaaagagac gctctacagt ttcgatcagc tgaaaacgaa
     8221 caagagcgaa ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga
     8281 actgaccagc ggtgagcgcg cgatcctgca cggtctggcg attgccgccg ggattaaagc
     8341 agcaaaagat ctcggcaata tgccgccgaa tatctgtaac gccgcttacc tcgcttcaca
     8401 agcgcgccag ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca
     8461 gcagatgaaa gagctgggga tgcattccta tctggcggtc ggtcagggtt cgcaaaacga
     8521 atcgctgatg tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt
     8581 gctggtgggt aaaggtttaa ccttcgactc cggcggtatc tcgatcaagc cttcagaagg
     8641 catggatgag atgaagtacg atatgtgcgg tgcggcagcg gtttacggcg tgatgcggat
     8701 ggtcgcggag ctacaactgc cgattaacgt tatcggcgtg ttggcaggct gcgaaaacat
     8761 gcctggcgga cgagcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaactgt
     8821 tgaagtgctg aataccgacg ctgaaggccg cctggtactg tgcgacgtgt taacttacgt
     8881 tgagcgtttt gagccggaag cggtgattga cgtggcgacg ctgaccggtg cctgcgtgat
     8941 cgcgctgggt catcacatta ccggtctgat ggcgaaccat aatccgctgg cccatgaact
     9001 gattgccgcg tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta
     9061 tcaggaacag ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg
     9121 ggcgattacc gcaggttgct tcctgtcgcg ctttacccgt aagtacaact gggcgcacct
     9181 ggatatcgcc ggaaccgcct ggcgttctgg taaagcaaaa ggcgccaccg gtcgtccggt
     9241 ggcgttgctg gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaattgcg
     9301 tcaggcaagg ctgttattgc cggatgcggc gtgaacgcct tatccgacct acacagcact
     9361 gaactcgtag gcctgataag acacaacagc gtcgcatcag gcgctgcggt gtatacctga
     9421 tgcgtattta aatccaccac aagaagcccc atatatgaaa aacgcgacgt tctaccttct
     9481 ggacaatgac accaccgtcg atggcttaag cgccgttgag caactggtgt gtgaaattgc
     9541 cgcagaacgt tggcgcagcg gtaagcgcgt gctcatcgcc tgtgaagatg aaaagcaggc
     9601 ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa agctttgttc cgcataattt
     9661 agcgggagaa ggaccgcgcg gcggtgcgcc ggtggagatc gcctggccgc aaaaacgcag
     9721 cagcagcccg cgcgatatat tgattagtct gcgaacaagc tttgcagatt ttgccaccgc
     9781 tttcacagaa gtggtagact tcgttcctta tgaagattct ctgaaacaac tggcgcgcga
     9841 acgctataaa gcctaccgcg tggctggttt caacctgaat acggcaacct ggaaataatg
     9901 gaaaagacat ataacccaca agatatcgaa cagccgcttt acgagcactg ggaaaagcag
     9961 ggctacttta agcctaatgg cgatgaaagc caggaaagtt tctgcatcat gatcccgccg
    10021 ccgaacgtca ccggcagttt gcatatgggt cacgccttcc agcaaaccat catggatacc
    10081 atgatccgct atcagcgcat gcagggcaaa aacaccctgt ggcaggtcgg tactgaccac
    10141 gccgggatcg ctacccagat ggtcgttgag cgcaagattg ccgcagaaga aggtaaaacc
    10201 cgtcacgact acggccgcga agctttcatc gacaaaattt gggaatggaa agcggaatcc
    10261 ggcggcacga ttacccgtca gatgcgccgt ctcggcaact ccgtcgactg ggagcgtgaa
    10321 cgcttcacga tggacgaagg cctgtccaat gcggtgaaag aagttttcgt tcgtctgtat
    10381 aaagaagacc tgatttaccg tggcaaacgc ctggtaaact gggatccgaa actgcgcacc
    10441 gctatctctg acctggaagt ggaaaaccgc gaatcgaaag gttcgatgtg gcacatccgc
    10501 tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag attatctggt ggttgcgact
    10561 acccgtccgg aaaccctgtt gggcgatacc ggcgtggccg ttaacccgga agatccgcgt
    10621 tacaaagacc tgattggcaa atatgtcatt ctaccgctgg ttaaccgtcg tattccgatc
    10681 gttggcgacg aacacgccga catggaaaaa ggcaccggct gcgtgaaaat cactccggcg
    10741 cacgacttta acgactatga agtgggtaaa cgtcacgcgc tgccgatgat caacatcctg
    10801 acctttgacg gcgatatccg tgaaagcgcc caggtgttcg ataccaaagg taacgaatct
    10861 gacgtttatt ccagcgaaat cccggcagag ttccagaaac tggagcgttt tgctgcacgt
    10921 aaagcagtcg ttgctgcggt tgacgcgctc ggcctgctgg aagaaattaa accgcacgac
    10981 ctgaccgtgc cttacggcga ccgtggcggc gtagttatcg aaccgatgct caccgaccag
    11041 tggtacgtgc gtgccgatgt actggcgaaa ccggcggttg aggcggttga gaacggcgac
    11101 attcagttcg taccgaagca gtacgaaaac atgtacttct cctggatgcg cgatattcag
    11161 gactggtgta tctctcgtca gctgtggtgg ggtcaccgta tccctgcatg gtacgacgaa
    11221 gcaggtaacg tttatgttgg ccgcaacgaa gaagaagtgc gcaaggaaaa taacctcggt
    11281 gccgacgttg ccctgcgtca ggacgaagac gttctcgaca cctggttctc ttctgcgctg
    11341 tggaccttct ctaccctagg ctggccggaa aacaccgacg cactgcgcca gttccaccca
    11401 accagcgtga tggtatctgg tttcgacatc attttcttct ggattgcccg catgatcatg
    11461 atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc aggtgccgtt ccacaccgtt
    11521 tacatgaccg gcctgattcg tgacgacgaa ggccagaaga tgtccaaatc caagggtaac
    11581 gttattgacc cgctggatat ggttgacggt atttcgctgc cggaactgct ggagaaacgt
    11641 accggcaata tgatgcagcc gcagctggcg gacaaaatcc gtaagcgcac cgagaaacag
    11701 ttcccgaacg gcattgagcc gcacggcacc gatgccctgc gcttcaccct ggcggcgctg
    11761 gcttctaccg gtcgtgacat caactgggat atgaaacgcc tggaaggcta ccgtaacttc
    11821 tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga acacggaagg tcaggattgc
    11881 ggcttcaacg gtggtgaaat gacgctgtcg ctggcggacc gctggattct ggcagagttc
    11941 aatcagacca tcaaagcgta ccgcgaagcg ctggacagct tccgcttcga tatcgctgca
    12001 ggcattctgt atgagttcac ctggaaccag ttctgcgact ggtatctgga gctgactaag
    12061 ccggtaatga acggtggaac cgaagccgaa ctgcgcggta ctcgccatac gctggtgact
    12121 gtactggaag gtctgctgcg cctcgcgcat ccgatcattc cgttcatcac cgaaaccatc
    12181 tggcagcgtg tgaaagtact ttgcggtatc actgccgaca ccatcatgct gcagccgttc
    12241 ccgcagtacg atgcatctca ggttgatgaa gccgcactgg ctgacaccga gtggctgaaa
    12301 caggcgatcg ttgcggtacg taacatccgt gcagaaatga acatcgcgcc gggcaaaccg
    12361 ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac gtcgcgtaaa tgaaaaccgt
    12421 ggcttcctgc aaaccctggc gcgtctggaa agcatcaccg tgctgcctgc cgatgacaaa
    12481 ggtccggttt ccgttacgaa gatcatcgac ggcgcagagc tgctgatccc gatggctggc
    12541 ctcatcaaca aagaagatga gctggcgcgt ctggcgaaag aagtggcgaa gatcgaaggc
    12601 gaaatcagcc gtatcgagaa caagctggcg aatgaaggct ttgtcgcccg cgcaccggaa
    12661 gcgatcatcg cgaaagagcg tgagaagctg gaaggctatg cagaagcgaa agcgaagctg
    12721 attgaacagc aggctgttat cgccgcgctg taatcaaaac acagtgatga aaacgaaggc
    12781 cggagcatgc tccggccttt tttatctctt acagaaatgg taaagaaggc ataattcccc
    12841 gtgaaatact ggctaagaag cctttatctg gtt
//
LOCUS       DAJTNA010000112        12865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTNA010000112 DAJTNA010000000
VERSION     DAJTNA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.113"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            8..877
                     /locus_tag="OO161_002705"
     CDS             8..877
                     /locus_tag="OO161_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5463722.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1080..1433)
                     /locus_tag="OO161_002706"
     CDS             complement(1080..1433)
                     /locus_tag="OO161_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5463723.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1571..3217)
                     /gene="groL"
                     /locus_tag="OO161_002707"
     CDS             complement(1571..3217)
                     /gene="groL"
                     /locus_tag="OO161_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5463724.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3261..3554)
                     /locus_tag="OO161_002708"
     CDS             complement(3261..3554)
                     /locus_tag="OO161_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5463725.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3830..5086
                     /gene="yjeH"
                     /locus_tag="OO161_002709"
     CDS             3830..5086
                     /gene="yjeH"
                     /locus_tag="OO161_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5463726.1"
                     /translation="MSGLKQELRLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPALGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCSVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGITT"
     gene            complement(5102..5578)
                     /gene="fxsA"
                     /locus_tag="OO161_002710"
     CDS             complement(5102..5578)
                     /gene="fxsA"
                     /locus_tag="OO161_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5463727.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5915..7351
                     /gene="aspA"
                     /locus_tag="OO161_002711"
     CDS             5915..7351
                     /gene="aspA"
                     /locus_tag="OO161_002711"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5463728.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7469..8770
                     /gene="dcuA"
                     /locus_tag="OO161_002712"
     CDS             7469..8770
                     /gene="dcuA"
                     /locus_tag="OO161_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5463729.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8886..9224
                     /gene="cutA"
                     /locus_tag="OO161_002713"
     CDS             8886..9224
                     /gene="cutA"
                     /locus_tag="OO161_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5463730.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKYHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9200..10897
                     /gene="dsbD"
                     /locus_tag="OO161_002714"
     CDS             9200..10897
                     /gene="dsbD"
                     /locus_tag="OO161_002714"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5463731.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLTIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10934..11509
                     /locus_tag="OO161_002715"
     CDS             10934..11509
                     /locus_tag="OO161_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5463732.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11616..11691
                     /locus_tag="OO161_002716"
     tRNA            11616..11691
                     /locus_tag="OO161_002716"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11649..11651,aa:Phe,seq:gaa)
     gene            11889..>12865
                     /locus_tag="OO161_002717"
     CDS             11889..>12865
                     /locus_tag="OO161_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5463733.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRATEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENVMNAS"
BASE COUNT         3115 a         3346 c         3149 g         3255 t
ORIGIN      
        1 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa
       61 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      121 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      181 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      241 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      301 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      361 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctagc
      421 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      481 ctcactgtta gatttcctgc cgttatatga cgttgactgc caggaaaatg gcaatctgga
      541 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      601 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      661 gcgtgaagca acgccgggta aagaagtaga ggcgatcact cgccgaatgc ttgatttcag
      721 cccacgactg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      781 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      841 tcaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
      901 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
      961 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1021 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccaggctc tggatcacat
     1081 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1141 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1201 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1261 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1321 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1381 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1441 cccataatca atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa
     1501 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgagg
     1561 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1621 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcactcggtg gtgatcatca
     1681 ggccagccac agaagccgcg tactgcagag cagaacgggt tactttggtt ggatccagga
     1741 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1801 cgccgccttt aacagtgtta gcaacaacag acggttcttc gccgcagttc aatacgatct
     1861 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     1921 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     1981 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2041 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2101 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2161 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcaa
     2221 tgatggtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2281 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2341 cgatatcctg cagcatagct ttacggcgat cgccgaagcc cggagcttta accgcagcaa
     2401 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc cagcgcttcg ccttcaacat
     2461 cttcagcgat gatcagcagc ggtttacctg ctttagcaac ggcttccaga accggcagca
     2521 tttcgcggat gttggagatt ttcttgtccg ccagcaggat gaacgggctt tccagttcta
     2581 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2641 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2701 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2761 tagcggagat ggtaccaacc tgagcaatcg ctttagagtc agagcatggt acggacagcg
     2821 ctttcagttc ttcaactgca gcggtaacag ctttgtcgat accacgtttc aggtccatcg
     2881 ggttcatgcc cgcagcaact gctttcagac cctcagtgat gatagcctga gccagtacgg
     2941 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt agaggcaact tctttcacca
     3001 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3061 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggcctttcg
     3121 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3181 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3241 tgttcagtgt cgtgcgagga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3301 atcaacactt cttcattgtc gatcttctca gatttcacac cgtagccatc gttgaaaata
     3361 acgatgtcgc caactttcac atccagcggc ttcacttcgc cattttcaag gatacggcca
     3421 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3481 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3541 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3601 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggat tcgccccttc
     3661 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3721 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3781 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3841 caaacaagaa ctgaggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     3901 cggcgtgttt gccgttcctg cgttagctgc acaggtagca ggcaataaca gcctgtgggc
     3961 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4021 ccactatccc agcgcaggcg gtgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4081 tgagcgagtc accggctggt tgtttttatc ggtcattccc gtgggtttgc cagccgcgct
     4141 acaaattgcc gccgggttcg gccaggcaat gtttggctgg catagctggc aactgttgtt
     4201 ggcagaactc ggtacgctgg cgctggtgtg gtatatcggt actcgcggtg ccagttccag
     4261 tgctaatcta caaaccgtta ttgccggact tatcgtcgcg ctgattgtcg ctatctggtg
     4321 ggcgggcgat atcaaacctg cgaatatccc cttcccggca cttggtaata tcgaacttac
     4381 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4441 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgcgcat tgatgattgg
     4501 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4561 ctatggtgaa aaaatggcgg cggcagcatc gcttccaaaa attgtagtgc aactgttcgg
     4621 tgtaggagcg ttatggattg cctgcgtgat tggctatctg gcctgctttg ccagtctcaa
     4681 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4741 ctacctggca cgtctctctt ctcgccatat cccgaataat gccctcaatg cggtgctcgg
     4801 ctgcagtgtg gtgagcactt tggtgattca tgctttagag atcaatctgg acgctcttat
     4861 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     4921 attattgcaa ggacgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     4981 actggcaatg gtcggctgga aaagtctcta tgcgctgatc gtgctggcgg ggttatggct
     5041 gttgctgcca aaacgaaaaa cgccggaaaa tggcataacc acataatccg gcgtttcgac
     5101 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg ctcatcatcc tttcgctggt
     5161 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatac
     5221 gagaaaagcg caaatgcggc atcaacttca ctgtcagatg cttttgcacc ggcggtaata
     5281 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5341 tgatcagcga aacactttta atcatctccg ccgctgggtt ttcacccgcc gccatttttt
     5401 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacatc ccgataactg
     5461 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5521 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5581 gtttctcctg taatagcagc cggtaaaacc cggctacctg aatgggttgc gaatcgcgtt
     5641 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5701 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa acgacactta
     5761 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5821 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtataaa
     5881 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     5941 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6001 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6061 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6121 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6181 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6241 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6301 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6361 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6421 actgcgtgaa ggctttgaac gtaaagctgt cgaattccag gacatcctga aaatgggtcg
     6481 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6541 cctgctgaaa gaagaagtga aaaatatcca acgtaccgct gaactgctgc tggaagttaa
     6601 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6661 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6721 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6781 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggcccac gtgccggcct
     6841 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     6901 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     6961 tgttaccatg gcagcagaag caggtcagct gcagttgaac gtaatggagc cggtcattgg
     7021 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7081 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7141 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7201 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7261 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7321 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaagaaggc
     7381 acgtcagatg acgtgccttt tttcttgtga gcagtaactt aaaaacaaca atctaatatc
     7441 aacttgttaa aaaacaagga aggctaatat gctagttgtt gaactcatca tagttttgct
     7501 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7561 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7621 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7681 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7741 acctatcgtg acctatttcc tgacaatctt tgctggtact ggcaacatct ctctggcgac
     7801 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7861 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     7921 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     7981 catcccgtcc accctgctgg cggttctggt gatgtccttt ctggtcacta tgctgttcaa
     8041 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggcc tggttgaact
     8101 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8161 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8221 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8281 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8341 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8401 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8461 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8521 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8581 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8641 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8701 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8761 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8821 tccttccccc gtacgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     8881 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtacggcac
     8941 cagatgaagc gacagcccag gatttagctg ccaaagtgct ggcggaaaaa ctggcggcct
     9001 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9061 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9121 gcctgaagta tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9181 gagacacaga ttacctctca tggctcaacg catctttacg ctgatcctgc tactttgcag
     9241 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9301 tcaagccttt acttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9361 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9421 tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9481 gatttaccgc gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac
     9541 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9601 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9661 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9721 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9781 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9841 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
     9901 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcaccatcg tctttacctt
     9961 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10021 tctcacgttg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
    10081 ggcgattgcc ggacttatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10141 gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt
    10201 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10261 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10321 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10381 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10441 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10501 ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa
    10561 aacggtagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10621 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10681 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacatca cggccaacga
    10741 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10801 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10861 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag cgggaaagac
    10921 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    10981 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11041 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11101 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11161 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11221 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctatccatca
    11281 actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacact
    11341 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11401 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11461 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11521 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11581 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    11641 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
    11701 caacggacct ctacggaggt ccgtttttcg tttcagaacg ccacgattta agcgttctgc
    11761 ctacaaatca attctaccga actcaaccag attcccccca catcaacccc attgtgtggg
    11821 tataattgcg ggcatacccc agttcgacag aatttgtacc ctctttaacg cattcagaag
    11881 gctgaactat ggcactgact gacgcaaaaa tccgggctgc aaagcccact gacaaggctt
    11941 ataaactcac tgacggggct ggcatgttcc tgctggtaca tcctaatggc tcccgttact
    12001 ggcgtctccg ttatcgtatt ctgggtaagg agaagactct ggcacttggt gtgtatccag
    12061 aagtttctct ctccgaagct cgtacaaaac gggatgaggc ccgaaaactg atttcggagg
    12121 gggttgaccc ttgcgaacag aaaagagcta aaaaagtagt ccctgattta cagctctctt
    12181 ttgaacatat tgcgcgacgc tggcatgcca gtaataaaca atgggcacaa tcacacagcg
    12241 ataaagtact caaaagcctc gagactcacg ttttcccctt tatcggcaac cgggatatca
    12301 caacactcaa taccccggat ctgcttatcc ctgttcgtgc tacagaagct aaacaaattt
    12361 atgaaatcgc cagtcgtctg cagcaaagaa tatctgccgt aatgcgttat gccgtacagt
    12421 ctggcatcat cagatataat cctgctctgg atatggctgg cgcattgact acggtaaaac
    12481 gccagcatcg ccccgctctt gatctttctc gcctgcctga acttttgtcg cgtattggca
    12541 gttacaaagg gcaacctgtc acccagcttg ccgttatgct gaatttactg gtttttattc
    12601 gttccagtga actcagatac gcccgctggt ctgaaattga tattgacaat gccatgtgga
    12661 ctattccagc cgaacgcgaa cccctgccag gcgtaaaatt ctcacaccgg ggctccaaga
    12721 tgcgaacacc acatcttgtg ccactcagca aacaggctgt agccatactg acagaactac
    12781 agacatgggc tggtgaaaat ggtctgatct ttacgggagc acatgacccg cgtaaaccaa
    12841 tcagtgaaaa tgtaatgaac gcatc
//
LOCUS       DAJTNA010000113        12791 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTNA010000113 DAJTNA010000000
VERSION     DAJTNA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12791
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.114"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..194
                     /locus_tag="OO161_002718"
     CDS             <1..194
                     /locus_tag="OO161_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5463734.1"
                     /translation="RGAFFISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGT
                     NTLLACCCLLRPEEVNSEY"
     gene            184..357
                     /locus_tag="OO161_002719"
     CDS             184..357
                     /locus_tag="OO161_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5463735.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(406..1479)
                     /locus_tag="OO161_002720"
     CDS             complement(406..1479)
                     /locus_tag="OO161_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5463736.1"
                     /translation="MAEKKRTRWQRRPGMTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIGCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1598..3352)
                     /gene="torS"
                     /locus_tag="OO161_002721"
                     /pseudo
     CDS             complement(1598..3352)
                     /gene="torS"
                     /locus_tag="OO161_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            3435..4424
                     /gene="torT"
                     /locus_tag="OO161_002722"
     CDS             3435..4424
                     /gene="torT"
                     /locus_tag="OO161_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5463737.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQDMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMVWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(4397..5089)
                     /gene="torR"
                     /locus_tag="OO161_002723"
     CDS             complement(4397..5089)
                     /gene="torR"
                     /locus_tag="OO161_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5463738.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            5209..6381
                     /gene="torC"
                     /locus_tag="OO161_002724"
     CDS             5209..6381
                     /gene="torC"
                     /locus_tag="OO161_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5463739.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDSHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCYG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            6381..8961
                     /gene="torA"
                     /locus_tag="OO161_002725"
                     /pseudo
     CDS             6381..8961
                     /gene="torA"
                     /locus_tag="OO161_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            8958..9557
                     /gene="torD"
                     /locus_tag="OO161_002726"
     CDS             8958..9557
                     /gene="torD"
                     /locus_tag="OO161_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5463740.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(9650..9955)
                     /gene="cbpM"
                     /locus_tag="OO161_002727"
     CDS             complement(9650..9955)
                     /gene="cbpM"
                     /locus_tag="OO161_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5463741.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(9955..10875)
                     /gene="cbpA"
                     /locus_tag="OO161_002728"
     CDS             complement(9955..10875)
                     /gene="cbpA"
                     /locus_tag="OO161_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5463742.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11136..12391
                     /locus_tag="OO161_002729"
                     /pseudo
     CDS             11136..12391
                     /locus_tag="OO161_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
BASE COUNT         3229 a         3102 c         3289 g         3171 t
ORIGIN      
        1 ctcgtggagc cttttttata tctgccttat ttttcttcaa cgctgtatgt atagtaagcg
       61 ataacctgtt gattattgaa tctttcgggg agatggctta taacatttct tacctgacca
      121 gggtaccggg aaccaacacc ttactggcgt gttgctgtct tttaagacca gaagaggtta
      181 acagtgaata ttgaagagtt aaaaaaacaa gccgaaacgg aaatcgccga ctttatcgcg
      241 caaaaaattg ccgagctgaa caagaataca gggaaagaag tctctgaaat tcgcttcacc
      301 gcacgagaaa aaatgaccgg gcttgaaagt tatgatgtca aaatcaaaat aatgtgattt
      361 tgtgaacatc accccgtgcg aggtgatgtt ccgcttgttg ctaatttagt gaccaatcat
      421 tggcgcttgt ggaattaagc gtcggtacaa ttcctccggc accgggcttt gccatactcc
      481 cgcatacatt gcgtaaccaa tcaccgcgaa cataatcccc agaaccagta gcgtcattaa
      541 ccagccagac aacgcaaaag cttttttatt tgccgcaggt ttttgcagtg aaaaggtcaa
      601 tgttgaggct accggacatg actctacgca agtcatacag ccggtacatt ccactgttcg
      661 tacctgaatt aatttatcta ccgggatccg tgatgggcaa ttttttgcgc atttgccaca
      721 gccgatacaa ctttcggcat tgcgacgaat cttaaacggc gataatagcg aaaccacgcc
      781 catcagcgcg ccatatgggc aaagataacg acaccaggca tggcgaataa acaggctggc
      841 aatcagcaaa acggtcacgc tgattaatgt cgcggtcccc atatgacgaa agaaatcgag
      901 catttttacg tccatcacca cgctgtaggg cgacaacata aaatagtgaa tcgcctgagc
      961 gggcatcaat aacgcgatat agagaaaaaa actcaacagc aaatacttca cgccgcgcag
     1021 aggaatatcc agccagcggg gaaggacaca ttgccgacca aacagtttgt taccgagatc
     1081 gccgattaat tcagaaagcg taccaaccgg gcataaccat gagcaaaagg cctttttgag
     1141 taatagactg atgacgataa aagcgaccaa taacagcatc gcggcggcgt ggacggacgg
     1201 taactgacct gttgtaaggc tatatttcag attcatcaga ccggcaatcg gtagccagcc
     1261 ttcgatacct cccggtctgg cgacaaatgt cgtgctactt gccgtttcgt aatagcgcac
     1321 ccaataccag aacgtgatgg caatataaat attcattgcc aacagtaata attgcgtcgc
     1381 tttacgccag gtcgtggcat tgcgccagtc attccacggt aatttgccgc ccgtcatgcc
     1441 tggccgccgc tgccagcggg ttcttttttt ctctgccatg attttgccag tccgttaagt
     1501 tgcataccaa atgccactat tctagttgtt cttaactggc tgatattgat tcaaatcgcg
     1561 ttcaggtctt tcttatgcaa ccatgcttcc agagcggcaa cactgcgtgt tgcccttcgt
     1621 cagtaaaacg cagggcgttg ctcaacaggt tggttataac ctgacgaata cgtcgcggat
     1681 cgcccattaa cgcggtcggt acatcgtcgg caattgctgt tgccaggcga atcgggcgac
     1741 ctttcacccg tccgctcatt aattgcaggg tactttccag cagcgggcgc ggttcaaagg
     1801 gctcatcgct gaccgaaaca ttcttgccac ctgcttcgat agccgaataa tcgagaatat
     1861 cgttgaggat ggtcagcaac gattcaccag agtcggtaat cgcccgcaaa tcatcacgct
     1921 gggcgttaag tgcggggtta tctgccagca gttgagcagt gccgagaata ccgtacagcg
     1981 gtgtgcggat ctcatggctc atcgccgcca gaaaggccga ttttgcctgg ctggcttttt
     2041 ctgcttccgc ccgtgcctgt cggtgttcta tcaccagttc ctgcaattca gctgtacgcg
     2101 ctttgacctg cgccgccagc tgttcacggt ggcgattcag tgcatgaaca gtgctgcgaa
     2161 acgcatccat cagccgcccg atggtatcca gctcccgtac gccagcggtt tccgggaaag
     2221 gggagtcaat atcaccgtcc agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac
     2281 gcgtgactga gcgataaacc acgcgccaaa ggatcagaat cagtgcgcaa agtgaaacca
     2341 tccccagcaa taacaggctg tattgcccgc gtgcactggc tttttccaga tgcgccagtc
     2401 cgtgctgatt acgcagctca atggtgtcga ccagctgact gacttcgcta ctaaactgcg
     2461 cgaacagggc gatgttattt tgtgcgagag tttgtaggtg attgctgatt tcactgtcct
     2521 gctgatacag cgccagcaaa tcgctatatt ggctaacggt agttaacgtt gttgcgacct
     2581 gcgcacgaac acccggatct tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat
     2641 tattgagctg cttttccagc gttggtgcat ttttctggat ctgctccagc cccagattca
     2701 tcaccatttg ctgcacccgc agagcgctaa ggcgcagttc attcatctgg ttaacatact
     2761 caagatcgat atcaatcagc cgatcgagtg cactttcagc agcctgacgc tgatcttgtt
     2821 cgatcaaatc gtaaatcccg gccagggtcg ctccagcgga agttgtcgca ttattcgcct
     2881 gaccttgcgc caggcgtgcg atctcatcgg cggcagcgac tatctgctga ctgagttgct
     2941 gttgttgctg gcgtagttgc agacgccgcc ccaccagttc cccttgctga cgtaacgaac
     3001 gggagatctc ctgctcctgt tgttcaatag cggtggtatc aaaaccttgt tcctgtaacg
     3061 cttgcagcaa cgcattaatc ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc
     3121 acatcttttc gttatcggca ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg
     3181 cttcgctcaa ctggcgcgcc atattcatgg taggaatcaa tgcctgagtg ttgtcttttt
     3241 ccacctggct gataaagcgc aggttgtacc atcccaccag ggtactggtc agggttaaca
     3301 gcgccatcag ggcaaagccc atccagagtc ttcgggtcag ggttaaattc acggtcggtg
     3361 cactttaggt gaaaaaggtt gagtcgcaaa gcagaatgca tctagcataa agccttatta
     3421 ttgatgaggc tatcatgcgc gtactgctat ttttacttct ttcccttttc atgttatcgg
     3481 cattttcggc tgataacctg ttgcgctggc atgatgcgca gcattacacg gtgcaagcct
     3541 ctacgccgct taaagccaaa cgcgcatgga aactgtgcgc gctttatccc agcctgaaag
     3601 attcatactg gttatcgttg aactatggta tgcaggaggc aggcggctac agccagttgg
     3661 ctacccagca agcacaaatc gaccagtgta aacagtgggg cgcagaggcc attttgctcg
     3721 gtagtagcac tacgtcattt cccgacctgc aaaagcaggt agcaaatctg ccggtgatcg
     3781 aactggtaaa tgctcttgat gatcccctgg tgaaaagccg cgttggtgta ccctggtttc
     3841 agatgggcta tcaacctggg cgatatttgg tgcaatgggc gcacggtaaa ccactgaacg
     3901 tgctgttgat gcccggaccc gataatgccg ggggcagtaa agagatggtc gagggttttc
     3961 gcgcagccat tgccggaagc ccggtgcgta ttgttgatat tgcgcttggt gataacgata
     4021 ttgaaatcca gcgtaacctg ttgcaggata tgctggaacg ccacccagaa atcgacgtcg
     4081 ttgccggaac ggccattgcg gcagaggcgg caatgggtga agggcgtaac ctgaaaacgc
     4141 cgcttaccgt ggtgtcgttt tatctttcac atcaggtgta tcgcgggctg aagcggggaa
     4201 gagtgattat ggctgccagc gatcaaatgg tctggcaggg ggaactggcg gttgagcagg
     4261 ccatcaggca attacagggg caatcggtgt ctgataatgt cagcccaccg attttagttc
     4321 tgacgccgaa aaatgccgac cgtgaacata ttcgccgctc gctgtcacca gggggatttc
     4381 gtccggtcta tttttatcag cacacatcag cggctaagaa ataaccttca ccatgttgcg
     4441 tcaccagtaa atccgcgctg agtttatgac gtaaacgacg aattaacaca tcgacggtgc
     4501 gcaggtcagg gttttccacc cgacgcgcag aaagcatacg tagcagacgt tcacggctga
     4561 gaatttcgcc cggattcgtc acaaatgcca ccaacatttc atactctgcg cgggtcagtt
     4621 taatcggctc gccatcccgc tccagcgtat ggcgcgacac attcaggcaa taaccggcaa
     4681 agcgatagca gttgtcctga gtgtgcggtt gagcttgtcg cgcgaggtcg attcgccaga
     4741 gcagattttt cacccgtact accagttcgc gcagttccag cggtttggtg acgtaatcgt
     4801 ctgcgcccat ttccagccca acaatacggt caatccgatc gctgcgtccg gtaaccagaa
     4861 taatccccac cgttgagcgt tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg
     4921 gtaagttgat atccagcaga attaaatcta ccggctgatt ctgcataatt tcccgcagcc
     4981 cggcaccgct cgctgtaacg gaaacggtat acccctcctg agtgaagtaa gattgtaatc
     5041 gcgcctgggt aaccggctca tcttcaacaa taacaatgtg atgtggcatc agagggtttt
     5101 actcattctg ttcatattct gccgtaagcc gttcatcctg accagtgccg ctgttcatat
     5161 ttgctcatta agatcgcttc actaaaccat aattttacag gggctattat gcggaaactc
     5221 tggaacgcgc tacgccgacc cagtgctcgt tggtcggtac tggcgctggt cgcaattggg
     5281 attgtgattg gcattgcgct gattgtattg ccacacgttg ggatcaaagt caccagcaca
     5341 accgaatttt gtgtcagttg ccacagtatg caaccggtgt atgaagaata taaacagtcg
     5401 gtgcatttcc agaacgcctc cggcgtgcga gctgaatgcc atgactgtca tatcccgccg
     5461 gatattccag gcatggtgaa gcgcaaactg gaagcgagca atgatatcta ccagaccttt
     5521 attgctcact ccattgatac acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag
     5581 cgtgaatggg cgcgaatgaa agaaaacaac tcggcaacct gccgctcctg ccataactac
     5641 gatgcgatgg atcatgcgaa gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg
     5701 aaagataatc aatcctgcat cgacagtcat aaaggtattg cccaccagtt accggatatg
     5761 agtagcggct tccgtaagca gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac
     5821 acgctgtact ctattgatat taagccaatt tatgcggcga aaggcgataa agaagcctct
     5881 ggttctctgc tgcctgcttc ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa
     5941 attgaaatta ccggctggac ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca
     6001 ggtaaacgca tctttgttgc ctcgattcgt ggtgatgtgc agcagcaggt aaaaacgctg
     6061 gagaaaacca ccgttgccga caccaatacc gagtggagca agttgcaggc cactgcgtgg
     6121 atgaagaaag gtgatatggt gaacgatatc aaaccgatct gggcttatgc ggattcgttg
     6181 tacaacggca cctgtaacca gtgctacggc gcaccggaaa tctctcactt tgatgctaac
     6241 ggctggattg gcacgctcaa cggcatgatt ggctttacca gtctcgataa acgtgaagaa
     6301 cgcaccttgt tgaaatatct gcaaatgaat gcgtctgaca tcgcaggtaa ggctcacggc
     6361 gataagaagg aagaaaaata atgaacaata acgatctctt tcaggcatca cgtcggcgtt
     6421 ttctggcaca actcggcggc ttaacagtcg ccgggatgct ggggccgtca ttgttaacgc
     6481 cgcgcagtgc gactgcggcg caagcggcgg ctgtcatctc gaaagagggc attcttaccg
     6541 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     6601 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat tgccggattg
     6661 ccggattgcc ggattgccgg attgccggat cacgtacaca acgcggcgcg tattcgttat
     6721 ccgatggtac gcgtggactg gctgcgtaag cgccatctca gcgatacctc ccagcgcggt
     6781 gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg acatgttcta tgaagaactg
     6841 gaacgcgtgc agaaaactca cgggccgagt gccttgctga ccgccagtgg ttggcaatcg
     6901 acggggatgt tccataacgc ttcggggatg ctggcgaaag ctattgcctt gcatggtaat
     6961 agcgttggta cgggcggaga ttactctacc ggtgctgcgc aggtgatcct gccgcgcgta
     7021 gtcggttcga tggaagtgta tgaacagcaa acctcctggc cgctggtatt gcagaacagc
     7081 aaaaccatta tgctgtgggg ctccgatttg ctgaaaaacc agcaagcgaa ctggtggtgc
     7141 ccggatcacg atgtttatga atattacgag caactaaaag cgaaagtcgc cgccggtgaa
     7201 attgaggtca tcagcatcga tccggttgtc acatccaccc atgagtatct ggggcgcgag
     7261 catgtgaagc acattgcggt taacccgcaa actgacgtgc cgctgcaact ggcgctggcg
     7321 tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc tcgctaacta ctgtgtaggt
     7381 tttgagcagt tcctgccgta tctgctgggt gagaaagacg gtcagccgaa agatgccgca
     7441 tgggctgaaa aactgaccgg cattgatgcc gaaaccattc gtgggctggc gcggcagatg
     7501 gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc agcgtatgca gcacggtgaa
     7561 cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg ggcaaattgg cctgccaggt
     7621 ggtggttttg gttttggctg gcactacaac ggcgcaggca cgccggggcg taaaggcgtt
     7681 attctgagtg gtttctccgg ctctacgtcg attccgcctg ttcacgacaa cagtgactac
     7741 aaaggctaca gcagcaccat tccgattgcc cgttttatcg atgcgatcct cgaaccgggg
     7801 aaagtgatca actggaacgg taaatcggta aaactgccgc cactgaaaat gtgtattttt
     7861 gccggaacta acccattcca tcgccatcag cagatcaacc gcattattga aggctggcgc
     7921 aagctggaaa cggttatcgc catagataac cagtggacct caacctaccg ctttgccgat
     7981 atcgtactgc ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta cggcaaccac
     8041 tccaaccgtg gcattatcgc catgaaacag gtggtgccgc cgcagttcga ggcgcgcaac
     8101 gactttgata ttttccgcga gctgtgccgt cgctttaatc gcgaagaagc ctttaccgaa
     8161 gggctggacg aaatgggctg gctgaaacgc atctggcagg aaggtgtaca acaaggcaaa
     8221 ggacgcggcg ttcatctgcc agcgtttgat gacttctgga ataacaaaga gtacgtcgag
     8281 tttgaccatc cgcagatgtt tgttcgccac caggcattcc gcgaagatcc ggatctcgaa
     8341 ccgctgggca cgccgagtgg cctgattgag atctactcga aaaccatcgc cgatatgaac
     8401 tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc ccacggtggg
     8461 cccggctcgc agaagtatcc gttgcatctg caatctgtgc atccggattt ccgacttcac
     8521 tcgcagttat gtgagtcgga aactctgcgt cagcaatata cggtagcggg taaagagcca
     8581 gtattcatta acccgcagga tgccagcgcg cgcggtattc gtaacggtga tgtggtacgc
     8641 gtctttaacg ctcgcggtca ggtgttggca ggggcagtag tttctgaccg ctatgcaccc
     8701 ggcgtggcac gaattcacga aggggcatgg tacgatccag ataaaggcgg cgagcctggt
     8761 gcgctgtgca aatacggtaa ccccaacgtg ctgaccatcg acatcggtac ttcgcagctg
     8821 gcgcaggcga ccagtgcgca cactacgctg gtggaaattg agaagtacaa tggagcagtg
     8881 gagcaggtga cggcgtttaa cggccctgtg gagatggtgg cgcagtgcga atatgttccc
     8941 gcgtcgcagg tgaaatcatg accacgctga cagcacaaca gattgcctgt gtttacgcct
     9001 ggctggcgca gttgttctcc cgtgaactgg acgatgaaca actgacgcaa atcgccagtg
     9061 cgcagatggc tgaatggttt tcgttgctga aaagcgaacc gccgctggct gcggcggtga
     9121 atgagctgga aaactgtatt gccacgctga cagtacgtga cgatgcccgt ctggaactgg
     9181 ccgctgactt ttgcggcctg tttttgatga ctgataagca ggccgcgctg ccgtatgcat
     9241 cggcctacaa acaggacgag caagagatta aacgcttgtt agttgaggca gggatggaaa
     9301 ccagcggcaa tttcaacgaa ccggcagacc atctggcgat ctatctcgag ttgttgagtc
     9361 atctgcattt ttcgctggga gaggggaccg ttcctgcgcg aagaatcgac agtttgcggc
     9421 aaaaaacact gacggcgctg tggcaatggt taccagagtt tgctgcgcgt tgccatcagt
     9481 atgacagctt tggtttttac gcggcactaa gccagttatt gctggtgtta gtggagtgcg
     9541 accaccaaaa caaataacgt cgtttatgcg cctgaaaaga cgcgtttagc gtcgcatcag
     9601 gcattatggc gcagttgccg gatgcgtcat cacatccggc aaacacacat cacggatgag
     9661 ctacaaaccg ggaaagccgc tggcgtagca ggcggttttc ctgcttcagg tgtgcaatat
     9721 catccattaa cgtcagcgcc accgcgatcc ccggccagtc cagagccagt tcatgacgca
     9781 ggcgtaccgc gcgttgcacc acaatggcgg catggtcgtc aaatacccag gttgtttcct
     9841 ggatctcacg cggttcaacc acccccaaac cgacaatttc attcaactcc tcttcagaga
     9901 tgccggtatg caggcaaaat tcggtaatag taaaagtcac cgtaacatta gccatcatgc
     9961 tttcccccaa tctttacgtg gatcaaaaga cgactgggcg tctgccagtt gctgccacag
    10021 cgcggcagtg ttttcatccg gtttcggcgg catcacgatt ttcagtaccg catacagatc
    10081 gccggtctgt tttttgctca ccagaccttt gcctttaacg cgcaatcgtt gcccggcctg
    10141 gctgcctggc gggatagtca gcaaaatgct ttctttcagt gttggaacgg tgactttagc
    10201 acccagcgcc gcttcccacg ggctaaccgg caccacaatt tccagatcct ggccgacaat
    10261 atcgaacagc ggatgtggcg caatatgaat caccagccac aaatcgccat ttggaccacc
    10321 gttttcgccc ggcgtcccct ggcctttcag acggatgcgt tgaccattgc cgacgcccgc
    10381 cgggatcttc acattcagcg ttttcgggat ttcctgttcg atcatgccaa aggcgttata
    10441 aaccggcagg ttatagctga tggtacgctt atgctcagtc agcgtttctt cgaggaatac
    10501 cgccacttcg atttcaatat cgtggccgcg tgcggcgggg cgttgacggc tctggcgggc
    10561 atgctgaccg aaaattgacg agaagatatc gtcaaaatct tcggcattaa aactctgacc
    10621 gtcgccatga tggaactgac ggttaaattg cggatcgttg cgatgttgcc acatctgatc
    10681 atactcagcg cgacgttgtt catcacttaa cacttcccag gcttcagcga cctctttgaa
    10741 gcgggcttcg gcatccggtt ctttgctgac atcaggatgg tatttacggg ccagtcgacg
    10801 ataggcggtc ttgattgtct tgagatcgtc cgtcggtttc acgcccatga tggcgtaata
    10861 atcctttaat tccatagcgt tatctcgcgt aaatcaacac aaattgaagg aacccctgta
    10921 aggtaactcc tataagtgta gggtaatcct caaaatttca tatgccaaca cagaatatgt
    10981 tattgaaatc atcgtggaga ggaggtcgcc atcaatatgg gttgctgaac atattttaaa
    11041 caggtgaaaa agggtgagcg atttttgata gttgaactag gcactttaag tttaactagg
    11101 gcgtcattat ttattaaatt ttatagacgc tatatatggg tagtaatata catggaatta
    11161 gttgcactgc aaataattat ttgaaacagg cctggaacga tataaaaaat gagtacgaaa
    11221 aaaatcaaac atattcaatc acgctttttg aaaacacact ggtgtgtttt atgcggttat
    11281 acaatgaact cagacgtaaa gtaaatgaag aggatactcc atgtctggaa tgtgaatcac
    11341 tagaaaaaga atttgaggaa atgcagaatg ataatgatct atcattattt atgagaacat
    11401 tgcgtactaa tgatacacaa atttattcag ggtttcagga ggtattacat atactataca
    11461 atatgttcaa aatgttgata ttgttagagt gtccttgccg ggcagaggtt cagagtctat
    11521 cacagatttt aaaggttatt attggtatgg ctttatggaa tatattgaaa acattactgc
    11581 gtgtgatgat gttttttctg agtattgttt agatgatgaa aatatgagta tccagccaga
    11641 acagataaat acgccgggaa tatctgattt agatactggt attgatttgt ctggcatatc
    11701 ttttattcag agtgaaatta ataaaacata tggattaaaa tacgctccag tagatggcga
    11761 tggatattgt ctgttaagat ccatactggt tttaaaagaa catgagtatt catgggcgct
    11821 gggcggccat aagacgcaaa agcaagtata cgaagagttt attaaaattg ttgataaaca
    11881 aacgatcgag gctcttgttg atacggcatt ctataatctc agggaagatg taaagacgtt
    11941 atttggcgtt aatctacaat ctgacaacaa aattcagggg cagggtggtt ttttatcatg
    12001 gagttttttg tcttttaaaa aagaatttat tgatagttgc ttgaatgaca aaaaatgcat
    12061 actgcattta cccgaattta tatttaatga taacaaggta cgccttgttt tagacaccga
    12121 cccagagcag agggttaatg aggtgaaaaa tttcctcact gctctttcag atagcatttg
    12181 ctcattattt atttttaata gtaatatggc atcaatctcc ttggggaatg aatccgtttc
    12241 aacagatgac gaccttgagt atggttattt aataaacact ggcaatcatt atgatgttta
    12301 cctccctcct gaactttttg ctcaggctta taagttaaaa aataaggaaa tgaatgcgca
    12361 actcgactat ttaaatcgtt atgcaattta atggcaaagg catatgctaa aaaccattgt
    12421 tattagtctc acactttttt attggtaaat attgtctctg tattggtaac gccgcagata
    12481 ttctgtttag ccacaggtgc aattatcagc ggcgtacgcg aggcaggggc taatcaggca
    12541 tagtttgcgt caaaccttgc ctgtttttga agatgtatat agaaaaacag gcgttcaaca
    12601 agccattttg cgaacctgtt cccggaaaaa agtcatattt ctgtcacact ctttagtgat
    12661 tgataacaaa agaggtgcca ggaatgaaca aaacgctaat cgccgcagct gtggcaggga
    12721 tagttttact cgcttcaaac gctcaggcac aaaccgtacc ggaaggctat cagctacagc
    12781 aagtgctcat g
//
LOCUS       DAJTNA010000114        12760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTNA010000114 DAJTNA010000000
VERSION     DAJTNA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12760
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.115"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(395..1183)
                     /gene="phoH"
                     /locus_tag="OO161_002730"
     CDS             complement(395..1183)
                     /gene="phoH"
                     /locus_tag="OO161_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5463743.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILVRNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1804..3075)
                     /gene="efeB"
                     /locus_tag="OO161_002731"
     CDS             complement(1804..3075)
                     /gene="efeB"
                     /locus_tag="OO161_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5463744.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(3081..4208)
                     /gene="efeO"
                     /locus_tag="OO161_002732"
                     /pseudo
     CDS             complement(3081..4208)
                     /gene="efeO"
                     /locus_tag="OO161_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
     gene            complement(4266..5096)
                     /locus_tag="OO161_002733"
     CDS             complement(4266..5096)
                     /locus_tag="OO161_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5463745.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            5132..5434
                     /locus_tag="OO161_002734"
     CDS             5132..5434
                     /locus_tag="OO161_002734"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463746.1"
                     /translation="MQRKVNRSDSDYHYGKEKYKRNVYKMMLECKQREIMPGDINDYS
                     RVERWDCLQRTMCSCRMPRKRHIQPTKLSRLNASELPCKTDKRVYQAISSIRDYQI"
     gene            complement(5765..7273)
                     /gene="putP"
                     /locus_tag="OO161_002735"
     CDS             complement(5765..7273)
                     /gene="putP"
                     /locus_tag="OO161_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR5463747.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(7432..7641)
                     /locus_tag="OO161_002736"
     CDS             complement(7432..7641)
                     /locus_tag="OO161_002736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463748.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            7696..11658
                     /gene="putA"
                     /locus_tag="OO161_002737"
     CDS             7696..11658
                     /gene="putA"
                     /locus_tag="OO161_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5463749.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLTEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTID
                     "
     gene            complement(11698..12336)
                     /gene="rutR"
                     /locus_tag="OO161_002738"
     CDS             complement(11698..12336)
                     /gene="rutR"
                     /locus_tag="OO161_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5463750.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            12624..>12760
                     /locus_tag="OO161_002739"
     CDS             12624..>12760
                     /locus_tag="OO161_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5463751.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     AL"
BASE COUNT         3121 a         3510 c         3195 g         2934 t
ORIGIN      
        1 gaattaatta agattaacct tacatttata atattatccc taaagaataa atcagaggaa
       61 tatattcggt gttaaacagt cagtcaaagt atagcccatt gttgattaaa cctcataagc
      121 aaaattggca ccagatctcc tgtacaggtt gaatggccga aaaaagatag gcagatgctc
      181 atttttttgg cttctggaag aaaaactctc cgcgctggca gtattacact ggtctgatgc
      241 tatgacgaaa aatgcctggt aagagtatct aaaccagact ccagttcagc ttatcaacac
      301 gctcaaagtc gcccccctga acgctaacct gaagtgaaaa tctgttgaaa taaaacaaag
      361 cccggttcgc ccgggctctg caccgataac acacttaact gtatgcatgc agcgtacgtt
      421 ggcaaagtgc cgaacgtacg caatcctctt ttccgaaccg gacgatccca accatttcat
      481 cttcttcgaa acgttccagc gcgtcactta atccggagca cacgccgcga ggcaaatcgc
      541 attgcgtgat atcaccgttg acgataaccg tcacgttctc cccgaggcgg gttaaaaaca
      601 ttttcatttg cgcggcagtc acattctgcg cctcgtcaag aatgacgact gcattttcaa
      661 aggtacgtcc acgcatatag gcgaacggcg cgatttccac cttccctatt tccggtcgca
      721 ggcagtactg cataaaggaa gcccctaagc gccggaccag cacgtcgtag accgggcgaa
      781 aatagggagc aaacttttct gcgatatcac caggtaagaa gccaagatct tcatcggctt
      841 gcagaactgg acgggtgacg ataatcctgt cgacatcctt atgtatcagg gcctctgccg
      901 cttttgctgc gctgatccag gtttttccgc acccggcttc gcccgtggcg aatatcagct
      961 gcttactctc aatagccttc agatagtgca attgcgcttc atttcgcacg aggatgggcg
     1021 aggtatcgcg actgtcgcgg gccataccaa tggcttctac gccgctcatc tgcacaagcg
     1081 aggtgaccga ttcttcttca cgctgcttat ggctgcgcga atcccgtctc agcacacgtt
     1141 ttgcctcgcg acgagctttg atcactgctt tttgtcttcc catggagagc accttgagtt
     1201 gtttgtattc atcacacgcg ccgttggcag cgtgattctg cgcacgaaca tcagagggtt
     1261 ggcttccttg taagccatag tttgcttttg gataaaatgc cgaaaaacgg ctacgcgcac
     1321 cgtttacggc gtcggtaaca catgaaaaga aaggatgagg ttgaaaatgc aaagtgacga
     1381 gatgactacc ggaggagaaa actccgcgag tggtggcgcg ttgattatct aaaacatgtc
     1441 cagtacagga cgttaccatc cgcgatctcc atagtgactg actatcactg ccgggaactt
     1501 ccgctgctac ttaataagta caacagatct cgcatttgtt gcaacaatat atttacttat
     1561 atttaactat aaaacactat ttcagtgaca ttagtttcta ctggaaagat gacagagtga
     1621 tgacagtgat gaaaaaagct gtgtgctttc agcaggattt gcatcaggca tcagtgcacg
     1681 atcgccggat gcggcgtgaa cgccttatcc agcctaccaa acccgcactg attgtaggca
     1741 tgataagacg cgcaagcgtc gcatcaggca tctgcgcaca aatgccttat ccgcctaaaa
     1801 acattaaacc cgcaataacg cgcttccgag ataatcgttc gcgtccttca ctcccggcag
     1861 cgaaaaaaaa taaccgccgc cgataggttt aacgtattcc tccagcgctt cgccattgag
     1921 ccttttttgt actgtcagga agcctttttc cagatcgtgt tggtagcaga caaacagcaa
     1981 ccccatatcc agttgtccgg agttggtgac ccccaacgaa taactgtagc cgcgacgcag
     2041 catcaggctg gactcactct ccgccgtgcg gggattcgcc agccggatat ggctgtccag
     2101 cgcgatcacc ttcccttccg ggtcgctggc gtaatcaggc acatcatgct catgctgcat
     2161 tcccagcggc gcaccggttt gcttatcacg gccaaaaatc gtctgctgtt ctttcagcgg
     2221 cgttctgtcc caaaattcca ctcgaaactg aatcaagcgt actgcctgat agctgccacc
     2281 gattgtccac gcaggctcct gctgatctgc cgttacccac accacttttt gcatcaactt
     2341 atcattctgg ctatcgggat tggcagtgcc gtctttaaag cccagcaaat taatcggcgt
     2401 ctctttgcct ttactacgcg ctgcgtgatc ggaaataaac ccttcccgct tccagcgcac
     2461 gctgagcaaa tccggcgtgt gtttgatgat atcgcgcagc gcatggatca ccgtgtcctg
     2521 ggtgttggcg caaatttgta gcaacacatc accatggcat aacgccgcat ccagcgagtc
     2581 gttggggaaa cgcgtcatct tctgcaactt tttcggcatc tgtggcgcaa ggccaaaccg
     2641 ctcatcaaac aatgagtgac ccaccgataa cgtgatggtg agattatcgg gcgcaatgta
     2701 gccgccaaga atgccggaat cgagtggtgg caggcgcgga tttggcgttt ctggtgccgc
     2761 tccgccctga gtcagaaaag caaaacgctg agtcaacaag cgaaacaacc gttcaagatc
     2821 ggctttatcg ctggcaagca catcaaacgc caccagcatc attgcggcct gttgtggcgt
     2881 caggatccct gcctgatgct caccataaaa cggctgtttc tcattgcgtg catccggtga
     2941 aagcgtaccc ggcgcacttt gcgttttttg tgcatgagcg accggacaac ttcccgccag
     3001 cgccagcgca cctatcactt tcagtaaacg tcggcgtgac ggttcattca cgccgttttc
     3061 atctttatac tgcataacgc ttaatccact cccagcacac cgcgaagttg cgccagatct
     3121 tccgccagcg cagtaatcgg tcctttcagt gcattccggt cggcatcagt caatttgtcg
     3181 taggtttcaa aaccgtcttt agtacggtat ttcgccagaa tggtatcgac ctttttaaag
     3241 ttggcatcga cttttgccag cagttccggg ttggcttttt gcagttgtgg acgcagcaaa
     3301 tcgacaattt tctgcgagcc ttcaacgtta gcctggaaat cccacagatc ggtgtggctg
     3361 tagcgatctt cttcaccgct aattttgctg gctgccactt cctcaatcag tccggctgcg
     3421 ccgccgacca cttttgaagg tgggaaagcc agttcactga tgcgtttttg caaatcgacc
     3481 acatctgtat aaagctgctc agcgtactga tccatccctt tggtggtgtt gtcgccaaac
     3541 aatgcttttt ccagacggtg gaaaccagtg aattttgggt cggcggcttt ttgctcgtaa
     3601 tcatcttcac gggcgtcaat gctgccatcc agatcggaga acagttcagc aatcggttca
     3661 atgcgctcat agtgctggcg cgtcggcgca tacagtgctt tcgctttttc gatatcgcca
     3721 gctttaatcg cgtcggtaaa ggctttggta tcggtcacca gctgcgtggt ttccgccatg
     3781 acatacgctt tatatgcagt aattgcacca ccaagactta acagcgcatc actttgcgcc
     3841 gcatccgccg ttgcctcacc tttgacgatc aacttccctt tcgggttagt cagcagaccg
     3901 caggtcatat cgtattcgcc aggctgtaaa ttcgccgtca ttttctggct aaaaccaggg
     3961 gcgatatttt cccgctcttc caccaccatc acgcctttga ggatctccca ctccagcgcc
     4021 ttctggctat ggttctgaat aatgaactgt gttttcccgg cgttaaccgt aatggtcatc
     4081 ggttagcact gcttatccgt cacggtcact ttgacctgcg gcacatcagc ggcgttagcc
     4141 ataaaagcag aagaaaacag cgcagccacg ctcaactgca atgcgttacg gcggaagtta
     4201 atggttatga cgagactatc cctttaaagt atgttgtaac taagcaagag tttgcgtcgt
     4261 atttgttacg cggagcgaga cgctgtcgcc cctgcgcgtg gtggcagagc aaatgccacc
     4321 agcgccggga tgagataaat aaaccagacg gcgacttcgc tgacgctcgg cgcttcctga
     4381 tagccaaaaa tcccttccat cagcgtgcca aacagcgagt gagttgagag caccgcactc
     4441 atatcgaagg cgatttcctg aaagtggttc cacaatccgg cttcatgaaa tgcgcgaatg
     4501 gcaccagctg ccagccctgc ggcgacgaag agaataaaca ggctggtcca tttaaaaaat
     4561 gcaccaagat tgaggcgaat accgccccag tagagcagaa agccgagcac cacggctgta
     4621 gcaagaccga gcattgcacc cagcggcggc cagatcccga catcttgttg aaatgccgcc
     4681 agcaggaaaa agaccgactc cagcccttcc cttgcaacgg caaaaaagac catcatcacc
     4741 agcgcccagc catgatgatt tccacgctgc aatgcgctat cgactgcctg ttccagttgc
     4801 actttgacgt tgcgcgacac tttgcgcatc cagaaaacca tccaggtaag gatcaccacg
     4861 gcgatcaccg ccacgatacc ttcaaacagt tcctgttctt tttgcggaaa ttcgccggtg
     4921 gtttcgttaa tgaagatccc caggcccagg cacaacgcag cggcaagcaa cacgccaatc
     4981 cacatcacac caatccatcg gcctcgctgg gtacgcttaa gatagctggc aatcaaactg
     5041 acaatcagcg cggcttcaag tccttcgcgc aacataatga gaaacggaac aaacatgcca
     5101 cccaccctta aacgtttttc tgagtcaatc tatgcaaaga aaagtaaatc gcagtgatag
     5161 cgattatcat tacggtaaag aaaaatacaa gcggaatgtt tataaaatga tgttggaatg
     5221 taaacaaaga gagataatgc caggggatat aaacgattat tccagggtag aaagatggga
     5281 ttgtttgcaa agaaccatgt gttcttgccg gatgccgcgt aaacgccata tccagccgac
     5341 aaaactgtcc agactcaatg catcagagct tccatgtaaa actgataagc gggtgtatca
     5401 agcaatttcg tccatccgcg attatcaaat ctaacttttt gcccggaaca taataacagc
     5461 tcgggtgttg gtggcgtcaa aatcgctgaa gcgttccccg aaggccggga cagcccacat
     5521 ggatgtgggc tgagggcgcg tcttacagga cgttacctcg cgcccgaccc ggtagccgaa
     5581 ggggataagc tgaaggtacc gcgcagcggc gattttgttc gccagagccc gggggtgcac
     5641 agggggcggc ggcgattggc cgccccctgc gcgctccctg caccattcaa tttatatggc
     5701 gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc gcaggctaag
     5761 tcccttagct ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca tcggcctcgg
     5821 caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac actacaatcc
     5881 cgatactgcc gaagatgaag cccggaataa tttcgtacag tcccagccag ccgaactgct
     5941 tccagacgat aaccgtcagc gcaccgatga tcatccccgc cagtgcaccg ttacgcgtca
     6001 tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca aagcctgccc
     6061 acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc gcaatcgcca
     6121 ccagcgccac caccagcacc atcacacgcc ctacccacac cagctctttc tggctggcat
     6181 gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac accagcagtt
     6241 ggcaacttaa ggttgacatt accgccgcca gaattgccga cagcagaatc ccggcaatcc
     6301 acgggttaaa cagaatttgc gccagttcga taaacacgcg ctcggcgttc tggtttaccg
     6361 cgcctgctac cgccgggtgc tcattaaagt aagcaatccc aaagaagccg acagccaccg
     6421 cccctgccag gcagaggatc atccaggtca tactgatacg acgcgcatgg acaatgctgt
     6481 ggtgagaatc cgccgccata aaacgcgcca gaatgtgcgg ctgcccgaag taacccagcc
     6541 cccaacccat cagtgagata atggcaacaa agttcagacc tttgagcata tcaacgtttt
     6601 cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca ctgataatga
     6661 cgataaccgg tgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca gtgtcagtcc
     6721 agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgccgca gctcccgccc
     6781 acagagccgt ttcgtagctc atgccaaagg tactttcaaa cagacgcgcg cctgccacaa
     6841 tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag ataatgcgca
     6901 aaatgcggct tttatcttca aaacgcccgg tgaaataatc cggcaatgtt aaggcgttat
     6961 tattgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg atccacgcgc
     7021 ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa accgcgccag
     7081 gcaaccccat taacagccag ccactcatat ccgacgcacc cgccgataat gccgtcacaa
     7141 atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt gatcgccagg
     7201 cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc aacatcggtg
     7261 tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa acttgccgtt
     7321 atatctgcaa ccggaacggg gtaacagagt ttatttttta ccagggcgac cgtatcctgc
     7381 cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat tttagtgctc
     7441 agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg cacctttcaa
     7501 aaatgttaac tgccgcagag aaaaagtctg agttattttt ttaatccctg tcatatcaat
     7561 ttcttttatt aacatttcat tcatttttaa gcttgctacg caggtcacat ttaacatggt
     7621 tgcacaaagt tgcaacatca tggatatttc acgataacgt taagttgcac ctttcagaac
     7681 aacaggagta atggcatggg aaccaccacc atgggggtta agctggacga cgcgacgcgt
     7741 gagcgtatta agtctgccgc gacacgtatc gatcgcacgc cacactggtt aattaagcag
     7801 gcgatttttt cttatctcga acaactggaa aacagcgata ctctgccaga gctacctgcg
     7861 ctgctttctg gtgctgccaa tgagagcgat gaagcaccga ctccggcaga ggaaccacac
     7921 cagccattcc tcgactttgc cgagcaaatt ttgccccagt cggtttcccg cgccgcgatc
     7981 accgcggcct atcgtcgccc ggaaaccgaa gcggtttcta tgctgctgga acaagcccgc
     8041 ctgccgcagc cagttgctga acaggcgcat aaactggcat atcaactggc cgataaactg
     8101 cgtaatcaaa aaaatgccag tggtcgcgca ggtatggtcc aggggttatt gcaggagttt
     8161 tcgctgtcat cgcaggaagg cgtggcgctg atgtgtctga cggaagcgtt gttgcgtatt
     8221 cccgacaaag ccacccgcga cgcgttaatt cgcgacaaaa tcagcaacgg taactggcag
     8281 tcacacattg gtcgtagccc gtcactgttt gttaatgccg ctacctgggg gctgttgttt
     8341 actggcaaac tggtttccac ccataacgaa gccagcctct cccgctcgct gaaccgcatt
     8401 atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg atatggcgat gcgcctgatg
     8461 ggcgagcagt tcgtcacggg tgaaaccatc gcggaagcgt tagccaatgc ccgcaagctg
     8521 gaagagaaag gtttccgtta ctcttacgat atgctgggcg aagctgcact gactgccgca
     8581 gacgcacagg cgtatatggt ttcctatcag caggcgattc acgccattgg taaagcgtct
     8641 aacggtcgtg gcatctatga aggtccgggc atttcaatca agctgtcggc gctgcatccg
     8701 cgctacagcc gcgcccagta tgaccgggta atggaagagc tttacccgcg tctgaaatca
     8761 ctcaccctgc tggcgcgtca gtatgatatt ggtattaaca tcgatgccga agaggccgat
     8821 cgtctggaga tctccctcga tctgctggaa aatctctgtt tcgagccgga actggcaggc
     8881 tggaacggca tcggttttgt tattcaggct tatcaaaaac gctgcccgtt ggtgatcgat
     8941 tacctggttg atctcgccac ccgcagccgt cgccgtctga tgattcgcct ggtgaaaggc
     9001 gcgtactggg atagtgaaat caagcgtgcg cagatggacg gccttgaagg ctatcctgtt
     9061 tatacccgca aggtgtatac cgacgtttct tatctcgcct gtgcgaaaaa gctgctggcg
     9121 gtgccgaatc taatctaccc gcagttcgcg acgcacaacg cccatacgct ggcagcgatt
     9181 tatcaactgg caggtcagaa ctactatccg ggtcagtacg agttccagtg cctgcatggt
     9241 atgggcgagc cactgtacga gcaggtcaca gggaaagttg ccgacggtaa acttaaccga
     9301 ccgtgtcgta tttatgcccc ggttggcaca catgaaacgc tgctggcgta tctggtgcgt
     9361 cgcctgctgg aaaacggtgc taacacctcg tttgttaacc gtattgccga cacctctttg
     9421 ccactggatg aactggtcgc cgatccggtc actgctgtag aaaaactggc gcaacaggaa
     9481 gggcaaactg gattaccgca tccgaaaatt cccctgccgc gcgatcttta cggtcacggg
     9541 cgcgacaact cggcagggct ggatctcgct aacgaacacc gtctggcctc gctctcctct
     9601 gccctgctca atagtgcact gcaaaaatgg caggccttgc caatgctgga acaaccggta
     9661 gcggcaggtg agatgtcgcc cgttattaac cctgcggaac cgaaagatat tgtgggcttt
     9721 gtgcgtgaag ccacgccgcg tgaagtagaa caggcgctgg aaagtgcggt taataacgcg
     9781 ccaatctggt ttgccacgcc tccggctgaa cgcgcggcga ttttgcatcg cgctgccgtg
     9841 ctaatggaaa gccagatgca gcaactgatt ggtattctgg tgcgtgaagc cggaaaaacc
     9901 ttcagtaacg ctattgccga agtgcgcgaa gcggtcgatt ttctccacta ctacgccgga
     9961 caggtgcggg atgatttcgc taacgaaacc caccgtccat tagggcctgt ggtgtgtatc
    10021 agtccgtgga acttcccgct ggctattttc accgggcaga tcgctgccgc actggcggca
    10081 ggtaacagcg tgctggcaaa accggcagaa caaacgccgc tgattgccgc gcaagggatc
    10141 gccattttgc tggaagcggg tgtaccccct ggcgtggtgc aattgctgcc aggtcagggg
    10201 gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtgc gcggggtgat gtttaccggt
    10261 tcaaccgaag tcgctacgtt actgcagtgc aatatcgcca gccgcctgga cgctcagggt
    10321 cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg cgatgattgt cgattcttca
    10381 gcactgaccg aacaagtcgt cgtggatgtg ctggcctcgg cgtttgacag tgcgggtcag
    10441 cgttgttcgg cactgcgcgt gctgtgcctg caagatgaga ttgccgacca cacgttgaaa
    10501 atgctgcgcg gtgcaatggc cgaatgccgg atgggtaatc cgggtcgcct gaccaccgat
    10561 atcggtccgg tgattgatag cgaagcgaaa gccaatattg agcgccatat tcagaccatg
    10621 cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa acagcgaaga tgcccgtgaa
    10681 tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac tggatgactt tgccgaattg
    10741 cacaaagagg tctttggtcc ggtgctgcat gtggtgcgtt acaaccgtaa ccagctacca
    10801 gagctgatcg agcagattaa cgcttccggc tatggtctga cgcttggtgt ccatacgcgt
    10861 attgatgaaa ccatcgccca ggtcactggc tcggcccatg ttggtaacct gtatgttaac
    10921 cgtaatatgg tgggcgcagt ggttggtgtg cagccgttcg gcggcgaagg gttgtccggt
    10981 accgggccga aagcaggcgg tccgctctat ctctaccgtc tgctggcgaa tcgcccggaa
    11041 agtgcgctgg cagtgacgct cgcgcgtcag gatgcagagt atccggtcga tgcacagttg
    11101 aaagccgcat tgactcagcc gctaaatgca ctgcgggaat gggcagcaaa tcgtccagaa
    11161 ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg caggaataca acgattgctg
    11221 ccggggccga cgggtgaacg caacacctgg acgctgctgc cgcgtgagcg cgtgttgtgt
    11281 attgccgatg atgagcagga tgcgctgact cagctcgccg ccgtgctggc ggtgggcagc
    11341 caggtactgt ggccggatga cgcgctgcat cgtcagctag taaaggcatt gccatcggca
    11401 gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa ccgctcaacc ctttgatgcg
    11461 gtgatcttcc acggtgattc ggatcagctt cgcgcattgt gtgaagccgt tgctgcgcgg
    11521 gatggcgcaa ttgtttcggt gcagggtttt gcccgtggcg aaagcaatat ccttctggaa
    11581 cggctgtata tcgaacgttc gctgagtgtg aataccgctg ccgctggcgg taacgccagt
    11641 ttaatgacga tcgattaaac aggtagccgg aggatgttac accctcctcc ggcatcttta
    11701 acgtggtcga atcccctcaa taataatccg ctgcacgttt tcaaccgttt gattgaaaaa
    11761 tacctcatcg cgcaacgttg cgcctgtcac cgcctccacc tgaggggcga aatcggcgta
    11821 atgttgagtg gaagcccaaa tcataaaaat caaatgctgc ggatcaatcg gcgcgagttt
    11881 gccgcttttg acccaaccgg caatcagcgc cgatttctca tcaattaatg ccttcaaatc
    11941 gcccgtcagt tcatccatta acagcggcgc gcctgccagc atctccatac agaacaggcg
    12001 cgaagcctgc ggataatcgc gtgagacttc cagcttcaga cggatgtact ctttgatcgc
    12061 cgccagcggg gcgaaatctt cacgaaacgc ttttaacggt gccagccaga tatcgagaat
    12121 ctgccgcagc acggcaatat acagcgcctc ttttgacgga aagtaataca gcagattggt
    12181 ttttgaaaca cccgccaact ctgcgatctg ctccagcctt gtgccgtgaa aaccgaattg
    12241 tgaaaaagtg tccagtgctg cgctaagaat cgcttttttc ttcgcgctta ctgcgcgcga
    12301 acgtttaccc gttgttttca ctgcgccttg cgtcatgcgc tctcccctct tgtagattgg
    12361 caacaggata gcaaaacccc cgcccggcct cgaccttctg cactctcatc gcgcacggat
    12421 gcatgtttta tgtgcaactg ttttgaccgt ttagtccact ttttaccaga tattttagat
    12481 aacacaatgc taacacatta ataacattca ctttttaaaa actggcatcc gctttgcaaa
    12541 caagccaata cgcggctgaa aaggatatgc aggatgcagc gccccgtctg actttcactt
    12601 tacgagacga agagaggttg atgatgaaaa ttggcgtatt cgtacctatt ggcaacaacg
    12661 gctggctcat ttcgacccac gcgccgcagt acatgccgac ctttgaactg aataaagcca
    12721 ttgtgcaaaa agcggagcat taccatttcg atttcgccct
//
LOCUS       DAJTNA010000115        12663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTNA010000115 DAJTNA010000000
VERSION     DAJTNA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12663
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.116"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(17..93)
                     /locus_tag="OO161_002740"
     tRNA            complement(17..93)
                     /locus_tag="OO161_002740"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57..59),aa:Met,seq:cat)
     gene            302..1399
                     /gene="mltA"
                     /locus_tag="OO161_002741"
     CDS             302..1399
                     /gene="mltA"
                     /locus_tag="OO161_002741"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5463752.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1476..2282
                     /gene="tcdA"
                     /locus_tag="OO161_002742"
     CDS             1476..2282
                     /gene="tcdA"
                     /locus_tag="OO161_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5463753.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2333..2776)
                     /gene="csdE"
                     /locus_tag="OO161_002743"
     CDS             complement(2333..2776)
                     /gene="csdE"
                     /locus_tag="OO161_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5463754.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2776..3981)
                     /gene="csdA"
                     /locus_tag="OO161_002744"
     CDS             complement(2776..3981)
                     /gene="csdA"
                     /locus_tag="OO161_002744"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5463755.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMLAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKIIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4173..4400
                     /locus_tag="OO161_002745"
     CDS             4173..4400
                     /locus_tag="OO161_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5463756.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4751..5668
                     /gene="gcvA"
                     /locus_tag="OO161_002746"
     CDS             4751..5668
                     /gene="gcvA"
                     /locus_tag="OO161_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5463757.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5687..6082
                     /locus_tag="OO161_002747"
     CDS             5687..6082
                     /locus_tag="OO161_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5463758.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKLKGVSHE"
     gene            6075..7175
                     /gene="rlmM"
                     /locus_tag="OO161_002748"
     CDS             6075..7175
                     /gene="rlmM"
                     /locus_tag="OO161_002748"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5463759.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7219..7950)
                     /gene="fucR"
                     /locus_tag="OO161_002749"
     CDS             complement(7219..7950)
                     /gene="fucR"
                     /locus_tag="OO161_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5463760.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8008..8430)
                     /gene="fucU"
                     /locus_tag="OO161_002750"
                     /pseudo
     CDS             complement(8008..8430)
                     /gene="fucU"
                     /locus_tag="OO161_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
     gene            complement(8432..9850)
                     /gene="fucK"
                     /locus_tag="OO161_002751"
     CDS             complement(8432..9850)
                     /gene="fucK"
                     /locus_tag="OO161_002751"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR5463761.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9847..11592)
                     /gene="fucI"
                     /locus_tag="OO161_002752"
     CDS             complement(9847..11592)
                     /gene="fucI"
                     /locus_tag="OO161_002752"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5463762.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(11625..>12663)
                     /gene="fucP"
                     /locus_tag="OO161_002753"
     CDS             complement(11625..>12663)
                     /gene="fucP"
                     /locus_tag="OO161_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5463763.1"
                     /translation="IITGLFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETA
                     ANPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPE
                     QLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSR
                     LARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIG
                     RFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIF
                     SLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFTVIFI
                     FARFRSQTATN"
BASE COUNT         3027 a         3166 c         3356 g         3114 t
ORIGIN      
        1 ttcatcaaat tttaattggt tgcgggggcc ggatttgaac cgacgacctt cgggttatga
       61 gcccgacgag ctaccaggct gctccacccc gcgtccgtgg atgcgcacta tactccgctc
      121 gcgttttcat gcaacctttt ttttaccccg atcacggatt tagcatgatt ttgaacaaaa
      181 acaaaccact aaagcctatt ttttgtgcaa aatttgcgtc agggagaggc tgctcatttc
      241 attaaacgcg gcggtttgtt atcttcgttg cgccttattt tttaacctga agaagagaac
      301 aatgaaagga cgttgggtaa agtaccttct tatgggcacg gttgtggcaa tgcttgccgc
      361 ctgctcttcc aaatcaaccg atcgcggaca gcaatataaa gacgggaaat ttacccagcc
      421 tttctctctg gtgaaccagc cagatgccgt tggcgcgccg attaacgccg gtgattttgc
      481 cgagcaaatt aaccatatcc gtaattcgtc accgcgtctg tatggcaacc agagtaatgt
      541 ttataacgcg gtgcaagagt ggctgcgcgc aggcggtgat acccgcaata tgcgccagtt
      601 cggcattgat gcctggcaga tggaaggtgc cgacaactat ggtaacgtgc agtttacagg
      661 ctattacacg ccggtaattc aggcgcgcca tacccgccag ggcgagttcc agtatcctat
      721 ttaccgtatg ccgccaaaac gtggtcgtct gccgtctcgt gcggagatct acgcgggggc
      781 attgggcgat aaatacatcc tcgcttacag caactctctg atggataact tcattatgga
      841 tgtgcagggt agtgggtata tcgactttgg tgatggcagt ccacttaact ttttcagcta
      901 tgcagggaaa aacggtcatg cctatcgcag cattggtaag gtgctgatcg accgtggcga
      961 agtgaaaaaa gaagatatgt cgatgcaggc gattcgtcat tggggcgaaa cacacagtga
     1021 agccgaggtt cgcgagctgc tggaacagaa cccgtctttc gtcttcttta aaccgcaatc
     1081 ttttgcaccg gtgaaagggg caagtgcggt gccgctggtt ggccgtgctt ctgttgcctc
     1141 cgatcgttca attattccgc caggtactac cttgctggca gaagtgccgc tgctggataa
     1201 taacggcaaa tttaacggtc agtacgaatt gcgtctgatg gtggcgctgg atgtcggtgg
     1261 cgcaatcaaa ggccaacact tcgatatcta tcaagggatc gggccggaag ccggacatcg
     1321 cgcaggttgg tacaaccact atggacgtgt ctgggtgctg aaaaccgccc cgggcgcagg
     1381 taacgtcttt agcggctgat gtgttattct gtataccgtt tttacggata cgacagggtg
     1441 agggataacc tcaccctttt taattctgag gtgctatgtc tgtggtaatt agtgatgcat
     1501 ggcgtcagcg ttttggtggc acagcgcgtc tgtatggtga aaaagcgttg caactgtttg
     1561 ctgacgcgca tatttgtgtg gttggtatcg gtggtgtcgg ttcctgggcg gcggaagcgc
     1621 tggcgcgcac ggggattggc gcaatcacgc ttatcgatat ggatgatgtg tgcgtcacca
     1681 ataccaatcg gcaaattcac gctctgcgcg ataacgtcgg gctggcaaaa gcggaagtca
     1741 tggcggagcg cattcgccag attaacccgg agtgccgtgt aacggtggtg gatgatttcg
     1801 tgacgccgga taacgtagcg caatatatga gtgccggtta ttcgtacgtg attgatgcca
     1861 ttgatagtgt acgacccaaa gcggcgctga ttgcttattg tcggcgcaat aaaatcccgc
     1921 tggtcacgac cggtggcgcg ggtgggcaga ttgacccgac gcagattcag gttaccgatc
     1981 tggcgaaaac gattcaggac ccgctggcgg cgaagttgcg cgagcgcctg aaaagcgatt
     2041 ttggcgtagt gaaaaacagt ataggtaagc tcggcgtgga ttgcgtgttt tctactgaag
     2101 cgctggtgta cccgcagtca gacggtacgg tgtgtgcgat gaaagccacg gcagaagggc
     2161 cgaagcggat ggattgtgca tctggatttg gcgcggcaac gatggtgacc gccacctttg
     2221 ggtttgttgc ggtttctcat gcgctgaaga agatgatggc gaaagcagcg cgtcagggtt
     2281 aaacaatcag gtcggatgcg acgcgaacgt cttatccgac ctggttctgt gcttaaacct
     2341 gcttcgccgc agcgataatt gcctcgctta acgcattcaa cccctggctg cgtgaggcgc
     2401 taagctgccc acgcaatccc agctcatcaa acaatgccag cggcgactgt gcctgtaatt
     2461 cggcggcggt tttcccctca acggcagtca acaacaccgc caacaggccg cgcacaatgc
     2521 gcccttcgct gtcgccaaag aagtgcattt taccgttttc agccacggta tatcccagcc
     2581 agacgcggtt ttcgcatccg gcaatctctt tagcctgcgc ttttaactcg tctggcaaag
     2641 ccggaagctg tttccccagc atgatcaact ggcgatattt atcttcccat tgcgtcaacg
     2701 gtgcgaaggt attgcgtaac gtttctgcgg ttacggttgt gccgaacgga tgtccggcga
     2761 attgcgggtt tgtcattaat ccaccaataa ttccagcgcg cggtcaacgg cattcaccag
     2821 cgcatccaca tcactctttg tattatatgg cgcaaaagag gcgcgcagtg tgccggttac
     2881 gcctaattct gccagtagcg gctgagcgca atgttgcccg gcccgcaagg caataccgta
     2941 ctccgccagt agcgtcacca tatcgctgtg atgaacgcca gcaaaatcaa aggccagcag
     3001 gctggaatcc tggcagcgga atgaacgaaa gcctggacgt ttcgccagcg catcttccgc
     3061 cagcgttgct aagctatggc tccagttttc ggcctggttg atatcgtaat ctgccagcca
     3121 ttccagcgcc gcgcttaatc ctatgacacc agccacattt ggcgttcctg cttccagttt
     3181 ccacggcgca gattgagtcg tgaagccgtc aaaactcact tcgtgaatta ttttgccgcc
     3241 acccagccag ggcgacatcg cttccagcag ttctgatttg ccatacagca cgccgatacc
     3301 cgtcgggcca tacagtttgt gaccagagaa agcatagaaa tcgatatcca gttgctgaac
     3361 atccgcgggg aaatgcaccg ccccctgagc accatcaacc atcaccacca tccctgctga
     3421 atgagcaaag gtaatcgctc gcgccagatc cgggcaaccg ccagtaacgt tcgacatctg
     3481 actcaacgcc agaatccgac tacggggagt aatcagttct ggcaacaaat cgacatccgg
     3541 cagtcgctgc gcattaagcg gcaatttcac cactttggct ccagtttgtt gggcgagcat
     3601 cagccagggg acgaggttgg cgtggtgttc tgccacgctg acaataatct catcgcccgg
     3661 ttgcagacgc ggacgcgcat agcattgtgc caccatgttg atggattcag tggtgccgcg
     3721 cgtccagacg atagttttat catccggtgc attcagtaat tgcgccactt tctctcgtgc
     3781 agcttcataa cgcgcggtca ggcgttgggc ttcggcaaac tggctgcgat ggacgtttcc
     3841 ggcgctcagg ctgtaaaact gtcgagtggc ttcaaccacg gcttcaggtt taagcgcggt
     3901 cgcggcgctg tcgagataga cgcccgcatc ctgtagtgcg ggaaactggg cgcgaaactg
     3961 cgcgggatta aaaacgttca tggtactcct cggcttgatg caccgatcgt gacgcaaaat
     4021 ttactttgat acaaggagag tgctaaccat cagaattatc tggatatcct ggcgaagccg
     4081 cgtaagtaac atatgctgaa tagtgttagg cgttaatttt agcctgttaa tcaatactat
     4141 taagatttat taatcgcttt aaggagaaga tgatgaaaaa gactgccgca attatttctg
     4201 cctgtatgct gacttttgcc ctgagcgcct gttccggttc gaactatgtg atgcacacca
     4261 atgacggacg taccatcgtc tctgacggca aaccacagac tgataacgat accggtatga
     4321 tttcgtataa agacgctaat ggcaacaaac agcagatcaa ccgtactgac gtgaaagaga
     4381 tggtcgaact ggatcagtaa ttcgcgatcg caaggtaaaa aaaagcaccg caattaggcg
     4441 gtgctacatt aatcactatg gacagacagg gtaaatgtac aggaagtgaa aaaaggtagc
     4501 tttgctacca tggtctgaat cgcagaccaa ttgcaaacac aacaacacaa catcacaacc
     4561 gtaagccaaa agttcaccag aacacgcatt ccgataaaac ttttcgttcc ggctcaggaa
     4621 gtgccgccac tataggtatt tgctggtaga agctcaacgg ataatttata atggctcaga
     4681 ttaaaaaaac taataggtca catagtgtga tctaattgtt aaattcattt aacatcaaag
     4741 tttaatagcc atgtctaaac gattaccacc gctaaatgcc ttacgagttt ttgatgccgc
     4801 agcacgccat ttaagtttca ctcgcgcagc agaagagctt tttgtgaccc aggccgcagt
     4861 aagtcatcaa atcaagtctc ttgaggattt tttggggcta aaactgttcc gccgccgtaa
     4921 tcgttcactc ctgctgaccg aggaagggca aagctatttc ctcgatatca aagagatatt
     4981 ttcgcaatta accgaagcga cgcgtaaact ccaggcccgt agcgccaagg gggcgttgac
     5041 ggtcagttta ctccccagtt tcgccattca ttggttggtt ccgcgacttt ccagctttaa
     5101 ttcagcttat ccgggaattg acgttcgaat ccaggcggtt gatcgtcagg aagataagct
     5161 ggcggatgat gttgatgtgg cgatatttta tggtcggggc aactggccgg ggctacgggt
     5221 ggaaaaactg tacgccgaat atttattgcc ggtgtgttcg ccgctactgc tgactggcga
     5281 aaaacccttg aagacaccgg aagatctggc taaacatacg ttattacatg atgcttcgcg
     5341 ccgtgactgg cagacatata cccgacagtt ggggttaaat catatcaacg ttcagcaagg
     5401 gccaattttt agccatagcg ccatggtgct gcaagcggct atccacgggc agggagtggc
     5461 actggcaaat aacgtgatgg cgcaatctga aatcgaggcc ggacgtcttg tttgcccgtt
     5521 taatgatgtt ctggtcagta aaaacgcttt ttatctggtt tgtcatgaca gccaggcaga
     5581 actgggtaaa atagccgcct ttcgccaatg gatcctggcg aaagccgctg ctgaacaaga
     5641 aaaattccgc tttcgttatg aacaataatt tacgtagggt acgaccatga ccagccgttt
     5701 tatgctgatt ttcgccgcca ttagcggctt catttttgtg gctctgggcg cttttggcgc
     5761 gcatgtgtta agtaaaacca tgggggccgt tgagatgggc tggatccaga ccggcctcga
     5821 ataccaggcg tttcatacgc tggcgatctt aggtctggcg gtggcaatgc agcgtcgcat
     5881 cagtatctgg ttttactgga gtagcgtttt cctcgcgtta ggcacggtgt tgttcagcgg
     5941 cagcctttat tgcctggcgc tgtcccatct gcgtttgtgg gcgtttgtca ctccggttgg
     6001 cggcgtgagc ttcctcgcgg gctgggcgtt aatgttagtt ggtgctatcc gtttaaagct
     6061 caagggcgta agtcatgaat aaggttgtat tgctgtgccg tccgggcttt gaaaaagagt
     6121 gcgccgcaga aattaccgat aaagccggcc agcgggaaat tttcggtttt gcccgcgtga
     6181 aagagaatgc gggttatgtc atttatgaat gttatcaacc tgatgatggc gataagttaa
     6241 tccgtgagct gccgttcagt tcattaattt ttgcccgcca gtggtttgtg gtgggggaac
     6301 tcctgcagca tttgccgcca gaagatcgta ttacccccat tgtcggcatg ttacagggcg
     6361 tagtagagaa gggcggtgaa ctgcgtgttg aagttgccga taccaacgaa agcaaagagt
     6421 tgctgaaatt ctgccgtaaa tttactgttc cgctacgcgc tgccttgcgc gatgccgggg
     6481 tgctggcgaa ctatgaaacg ccgaagcgtc cggttgtgca tgtattcttt attgcaccag
     6541 gctgctgcta taccggttac tcatacagca acaataattc gccgttctat atgggcattc
     6601 cgcgcctgaa atttccggca gaggcgccga gtcgttccac gctcaaactg gaagaggcat
     6661 ttcatgtgtt tattcctgcg gatgagtggg atgaacgcct ggcgaacggg atgtgggcgg
     6721 tggatttagg cgcttgccct ggcggctgga cctaccaact ggtgaagcgc aacatgtggg
     6781 tttattccgt cgacaacggc ccgatggcgc aaagtctgat ggataccggg caggtgacgt
     6841 ggctgcggga agacggtttc aaattccgtc cgacacgcag caatatctcc tggatggtat
     6901 gcgatatggt tgaaaaaccg gctaaagttg cggcattgat ggcgcagtgg ctggttaatg
     6961 gctggtgccg tgaaaccatt ttcaatctca aactgccgat gaaaaaacgc tacgaagaag
     7021 tatcacacaa tctggcgtat attcaggcac agcttgatga acatggcata aatgctcaga
     7081 ttcaggcgcg gcagttgtat cacgatcgcg aagaagtgac ggtgcacgtc cgccgcatct
     7141 gggctgcggt gggtggtcgt cgcgacgagc gataacaggc aaggccgggg cgtcgtcttc
     7201 ggcctgctac cctttccgtc aggctgttac caaagaagtt gcaacctggc gctcatcaga
     7261 aataatgtgc gttacctcat ccagatgccc gatgcgggct tcccctgaac gattaaattt
     7321 acttttatca atcaataaca acgattgcgc ggcacgtttt aatagcatcg atttgtaatc
     7381 agcgttgatc gcattggagt cccacagtgc gccgctgcta tcgatccctt cacaagaaaa
     7441 aataaacaga tcgatttcca gcgatttaag ttgggaaatc agcgagggat tgacgtaaca
     7501 gccatatttg cgctcaagtg tgccgccgga actgatcagt tgaatgcgtt cgcgtttacc
     7561 gagttcatgg caaatcggat ggctattggt gaagacctga atgttgatgt caggcaactg
     7621 gcgtgccaga taccagcaag ttgaactggc gtctaaggct atcaccatcc cttcttcaat
     7681 ccacgcgagc gcctcgcgcg cgatatctgc tttatgcgca taatggcttt tcagcctgat
     7741 gtgaaaggga tcgccactgt cttgattttg acggtggata tatttagcgc gtccatgatt
     7801 gcgcagaatt ttaccctgcg tctgtaattc attgagatcg cgacgaatgg tttctttact
     7861 gacctttagc tgttcagaga gagcttccgt ggtcaggctg gtatggttca gcagcaggtc
     7921 gactatcgct tgctggcgtg ccgctttcat atttttcacc ccatcattcg ataaccgccc
     7981 gcgtcatgcg ggcgtaccgg tatgagatta cggtgttacc ccttttttta aaagaatatt
     8041 cccgtacttc gctcgttcgc ctgtgataac gatcgcaaag gctttttgcg cccgttcata
     8101 aaacgcaaaa cgattgatgc ggatgatgtc aggacacggg gcttgtagtg aaagcgcatt
     8161 acggtaacgt cgttctactt caggatcgag agtgtcacct tctaccgccg ccatcatcac
     8221 cagcggcggt gcataactgt ccagttcaaa taacgggata atcgcctgga gcaagtcgct
     8281 caccaacagg ccatcagcgc ggatcacctg cggtcccatc gaatgggcgg gaaagtgagc
     8341 atcggaaaaa ataatttaat ctccatgtcc catctctgcc agcactttca atagttcggg
     8401 agaaattaac ggcgaaattg ttttcagcat ttcacacttc ctctataaat tcaggttcag
     8461 tttgcgggta gaaataacgg tactgataat gaatctgtgc gcgggcttct tcagggctgt
     8521 taaattcccc tacgccatac caaccgaaca gcgcagctcc tgcgacggtc gtttcggcgt
     8581 cgtcgagaac ttttaccgga atatcaagca tattggcttt aatctgattc cacaatgtgt
     8641 tgcgacttcc tccaccgact aacaataatt cagaggcctt aaagtgcccg attttttcca
     8701 gcatctgtag attgcgctgt aattgcgcag ttaacccttc cagcgccgcg cgatagaaat
     8761 gcccccgcgt ggtattaagc gtcactcctt gccagccagc gttctgacac gacaataaat
     8821 cacactgcat ttttacgcca tccgcgctag gcgcgatcag acgagcttct tcaatcaaca
     8881 tttgccaggg tgtttcagcc gtccagaaca gttttctcac ccattccagc acgccggatg
     8941 ccagccattg catacctggg ttatacaacc ctgcctggct atccagttcg caggtggaac
     9001 cggcgtactg acttaacagc gaagtatcaa cctgggcgct acggaccatt aaaatttccc
     9061 atgtaccgga agagagcacg ggttcatttt ggtcggcacc agcgccaaaa agggcgaact
     9121 gggtatcgtg acctgcggaa atcaccggta tgccaacggg taagccgagc attgctgcgg
     9181 cgctgttctg tagcgtacca atctgttcac ccgcttccac cagacgaggg aagagtcggc
     9241 gtggaatacc agtggcttgt aaaatttgcg gactgaaatc gcgttgctgg atatccagca
     9301 tctggctggt tccggccatc gtgatatcag tagtgaattc gccggttaaa cggtggttaa
     9361 tcagcgacga aataaagaac caggcgtgcg cgcgttccag cagttgtgga tgattttctt
     9421 tcaaccacac caacttatat aacgtattga aactaaaggc tccgacgcca gaaatagcct
     9481 gcaaccgctg tgcggagatc aactgttcaa tattgtccat aaccgctgct gttcgcggac
     9541 atttccagct aatgatcgga tagagcagat tgccttgctt atctaccaga gcgccatcca
     9601 caccaaaggt ggtgacggcg ataccgcgga tgtggcattc agtcagttca ctattgattt
     9661 gccgacagca atcagcaaag cgttgcaaaa tggcgtctaa agaccactgg tgccaggtgt
     9721 tgttttccat cgcgatatcg ctggcattag gcgttgaggc gcgggcaaca atttttccct
     9781 gccgattaac cgcgatggcc ctgacattgg tcgcgccaca gtcgagtacc aggataactt
     9841 cttgtttcat aatcgctcct gcaatacttc aatatccatg ccgtgcgcag cccaggcaga
     9901 aggacgatac actttggtct cttcaacgtt gtgcatacat accgggatac gcagcatgga
     9961 ggcgagagtg ataaagtcag cgccaacgtg gccgatggtc agaaccccat ggttagcgcc
    10021 ccagttcgcc attaccgagt acacatccgt aaacggccct ttaccggtga ggcgcggtgc
    10081 aaaccaggtg gttggccagg ttgagttggt gcgtttgttg aggatgtcat gcacatcctt
    10141 cggcaattcc acgctccagc cttccgcgat ttgcagtacc ggtcccaggc ctttgatgat
    10201 gttgacacga gtcatggtga acgggacgcc gccttcggta aggaagcggg aagagtaacc
    10261 gccgccgcgg aagtattcgt ggatcgccgg gcaccattcg gtagcggcga ggcaagcgtc
    10321 agcctcttgc tgagagattt cccagtgcgg cttcatcgtc gggttacctt cgctgtcgcg
    10381 ttgtttacag gaaccgtcca gcgcagcaga acctgagttg atcaaatgga tgatgccgtg
    10441 ttctgccagt ccatcgagtt tatgccccgt tacacgctca attgcttctg gtgaccagta
    10501 ggtacgcaca tcggcaaata cctgagcggt gccggtgagc tggtgaccca ttagcattgc
    10561 cacgccgtta agactgtcgt tttcggtcgc cacgacaaag ggttcgcgca cgccattcca
    10621 gtcaaatgaa ctgttgagga tcgcttcggc ggtgtcacca ttgggatatt gatcggtcca
    10681 gtgacgttgc ccctggaagc ccgcagcgat ggcgttgtag ccaagtgatt cttccacgcg
    10741 accaatatcg gccagtttgc tgttgccttg catcatgtcg cggatacaca tcgccatcag
    10801 taaactttcg cgcagaactg cgcggctttg ctcggcattg cgttgatact gtttgttatt
    10861 ttcatcttcg ccatagcgga agtttttatc agcccaggcc agtgccattt ccagttcggc
    10921 ttcgtcgtaa atcttctgat cgatacggcg acgcagttca gtcatatcca ccgcctggac
    10981 tttcattccc agccaggatt cgaagaagtt gtgatcaacg atggaaccgg cgatacccat
    11041 cgaaacgccg cccagcgaca gatagctttt acctttcatg ctggcgacgg ccaaaccggc
    11101 gcgggcaaag cgcagcagtt tttcttcaac atcggcagga atcgatgtgt catcggcatc
    11161 ctgaacgtca tgaccgtaaa tggagaatgc cgggatgcct ttctggctgt gagctgccag
    11221 agccgctgcc aggtaaacag cgccgggacg ttcagtgccg ttaaagcccc aaatggcttt
    11281 cgggcgggtt ggatccatgt cgatggtttc actgccatag caccagcaag gcgttaccgt
    11341 aatggtgagg cctacattct ggctgctgaa tttttcttcg caagcagcgg cttcagccat
    11401 acccgcgata caggtatcgg aaatgacaca ctcgacggca gctccgcagg catggcgcag
    11461 tttctcggtc agcagtgcgg ccgtagcttt cgccatattc attgtttgtt cttcaagcga
    11521 ctcacgaaca cccatgcgac gaccgtcaat aaccgggcgg ataccaattt tcggtaagct
    11581 gatttttttc attacagatt cctcacttta ttcggaaaat atgttcagtt agttgccgtt
    11641 tgagagcgga aacgggcaaa gataaagatg accgtgaagc agagtgcggg gatcagttca
    11701 gcagtgggga tgttgcccgc cgcgtcactg acaaaaccca tgaccggagt gacaataccg
    11761 ccgccaataa tggtcataac gatgaaggac gaaccgtatt tggtgtcctg gccgagattc
    11821 ttaatgccca gcgagaagat tgttgggtac tgaatcgaca taaaggcgct gcataaagtc
    11881 agggctatta agcccacatg accgccagcg aaggctgaga tcaggcacag tgccatagcg
    11941 attaatgcgt aggcggccag gactttgtgt ggtgcgaagc gactgatgag ccaggtaccg
    12001 gtgaaacgac caataaagaa gcacaccatg gttccggtta aatagttagc ggcaaagcct
    12061 gcagtcatac ctggaatttc ttctacagcg tagcgaatca aatagctcca gcaggccgtt
    12121 tgtgcgccaa cgtagcagaa ttgcgctaat actgcccagc gccagtggcg aatacgcgcc
    12181 aggcgagaaa gcgatgcgga gaacgatcct tgtttggcgt cactgtgatt atcactctgc
    12241 aatgccggga atttcgtcag catgatcagc agggcgacca gtaacacgat agccacgatg
    12301 atcatataag gtgtctgtac cgataatacc aggctgtgtt tatacgcact caattgctct
    12361 ggagacattt tatcgagaac gtcttgcgat tgatgtggca cgttagacaa aataagactt
    12421 tgcccaaaga caaccgcgat aattgcgcca aacgagttaa atgtttgcgc aagatttaag
    12481 cggaagtgac cactgctttc cggccctaat accgtaacaa aagggtttgc ggcagtttcc
    12541 agacaaccta atccggctgc aataataaat aggccaacta aaaataaggt gtagttcatt
    12601 atttctgcag cgggccagaa taatgcagcg cccagggcat ataaaaataa cccagtaata
    12661 att
//
LOCUS       DAJTNA010000116        12646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTNA010000116 DAJTNA010000000
VERSION     DAJTNA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12646
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.117"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(6..326)
                     /gene="chbB"
                     /locus_tag="OO161_002754"
     CDS             complement(6..326)
                     /gene="chbB"
                     /locus_tag="OO161_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5463764.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(624..962)
                     /gene="osmE"
                     /locus_tag="OO161_002755"
     CDS             complement(624..962)
                     /gene="osmE"
                     /locus_tag="OO161_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5463765.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1164..1991
                     /gene="nadE"
                     /locus_tag="OO161_002756"
     CDS             1164..1991
                     /gene="nadE"
                     /locus_tag="OO161_002756"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5463766.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2221..3108
                     /gene="cho"
                     /locus_tag="OO161_002757"
     CDS             2221..3108
                     /gene="cho"
                     /locus_tag="OO161_002757"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5463767.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3068..3643)
                     /gene="ves"
                     /locus_tag="OO161_002758"
     CDS             complement(3068..3643)
                     /gene="ves"
                     /locus_tag="OO161_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5463768.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3846..4316)
                     /gene="spy"
                     /locus_tag="OO161_002759"
     CDS             complement(3846..4316)
                     /gene="spy"
                     /locus_tag="OO161_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5463769.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4646..5614)
                     /gene="astE"
                     /locus_tag="OO161_002760"
     CDS             complement(4646..5614)
                     /gene="astE"
                     /locus_tag="OO161_002760"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5463770.1"
                     /translation="MDNFLALTLTGKKTVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5607..6950)
                     /gene="astB"
                     /locus_tag="OO161_002761"
     CDS             complement(5607..6950)
                     /gene="astB"
                     /locus_tag="OO161_002761"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR5463771.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVTRLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6947..8409)
                     /gene="astD"
                     /locus_tag="OO161_002762"
                     /pseudo
     CDS             complement(6947..8409)
                     /gene="astD"
                     /locus_tag="OO161_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(8406..9440)
                     /gene="astA"
                     /locus_tag="OO161_002763"
     CDS             complement(8406..9440)
                     /gene="astA"
                     /locus_tag="OO161_002763"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5463772.1"
                     /translation="MMVIRPVERSDVSTLMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(9437..10657)
                     /gene="astC"
                     /locus_tag="OO161_002764"
     CDS             complement(9437..10657)
                     /gene="astC"
                     /locus_tag="OO161_002764"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR5463773.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIMAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTVKALGGDFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11103..11909
                     /gene="xthA"
                     /locus_tag="OO161_002765"
     CDS             11103..11909
                     /gene="xthA"
                     /locus_tag="OO161_002765"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5463774.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12076..>12646
                     /locus_tag="OO161_002766"
     CDS             12076..>12646
                     /locus_tag="OO161_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5463775.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITL"
BASE COUNT         3073 a         3419 c         3097 g         3057 t
ORIGIN      
        1 ataaattaat ttgctgcggc ttttttaatc gctgcaaccg cagccttaag cacgcctaaa
       61 ccatcgactt tgccataaag cagcgagtca attacttcaa ccggtttgtt gggtaacaaa
      121 cgctggattt cgggcaacat ataagcaatc tgcggcccta ataacacgac atcggcattc
      181 tgaccttttt caccagccag tgtttccgga aatgcttcaa taatgaccgg aacttcatat
      241 ttttctgcct gtgcgcgcat ttttgatacc agtaaagagg tagacatgcc cgcagaacaa
      301 aacagataaa tgtgtttctt ttccataaaa ctgccctcat cgacgattat ctgtcagcca
      361 gacactccgc aagccttaac ctgcttccat gctctgggta acttgcgaaa ccaacatgac
      421 tttttttgtg gctgaaagga gtatacggta tagacctgag caatagtatt tctttgacct
      481 tataaattgt ctctccttga cctgcggtta tgaccctctt cacatttcgg gcaaataatt
      541 cgcgaagata attaagaact caggccaaaa aaaccggcgc aatggccggt ttccgttgtt
      601 actcaagctt tcaaacgaag tgattacttc gcagcctgtg gatcagtgtc gtattcagca
      661 caggtctgat aaccggagtt gatgacatgt ccagtatcat ctaacgcgac aaagtaggtt
      721 tctgctttac catcacgttg acccaggatg taggtctggc aagtaccacg agcatggatc
      781 atgctcactt cagacgaagg tttacccgca atttgtgcaa cctgcgcccg gctcatgcct
      841 tttttgacgt ctttcaccac aggctgtaca aactggtctt tggtacgatc ataagccgta
      901 caacccgcca gcatggttaa taccgccgct gcactcagaa ttcctgccat attcttgttc
      961 atattccgtc ctcttgttta tcagcgtgtt agataagcct ggaatacatt gggcgctttt
     1021 tcaagcccgt gaacgaaacg gctccgcttt cagaggattc ctgtatgacg ttttatccac
     1081 cattcaaccc gctgtcgctt gtcgtttcag tagcgacggg ttagctttaa ggaagttttg
     1141 tcttttctgt ctggaggggt tcaatgacat tgcaacaaca aataataaag gcgctgggcg
     1201 caaaaccgca gattaatgct gaagaggaaa ttcgtcgtag tgtcgatttt ctgaaaagct
     1261 acctgcaaac ttatccgttc attaaatcac tggtgctcgg gatcagcggc ggtcaggact
     1321 ccacgcttgc cggtaagctg tgccagatgg cgattaatga gctgcgccag gagaccggca
     1381 acgaatcact gcaatttatt gccgtacgcc tgccctatgg tgttcaggcc gacgaacaag
     1441 attgccagga tgccattgcc tttattcaac cggatcgcgt attaaccgtt aatatcaagg
     1501 gcgcggtatt ggccagcgaa caggcgctgc gcgaagcggg cattgaactg agcgattttg
     1561 tccgtggcaa tgaaaaagcg cgtgagcgga tgaaagcaca atatagcatt gcgggtatga
     1621 ccagcggtgt cgtggtgggc accgatcatg cagcagaagc tattaccgga ttcttcacta
     1681 aatatggtga cggcggtacg gacattaatc cgctgtatcg tctcaacaaa cgtcagggta
     1741 aacagttact ggcggcatta gcttgcccgg aacaccttta taagaaagcg ccaacggccg
     1801 atctggaaga tgatcgccct tctctgccag atgaagtggc actcggcgtg acctatgaca
     1861 atatcgacga ctatctggaa gggaaaaacg tacctcaaca ggtcgccaga acaatagaga
     1921 actggtatct gaaaaccgaa cataaacgcc gtccgccaat taccgttttc gatgatttct
     1981 ggaaaaagta ataatttgca catattggat tgtgcgaaaa agagtaattt gttcacgccg
     2041 gatgcggcgt gaacgcctta ttcgacctat aaaactatgc aaattcaata cattgcagga
     2101 gtcgaatagg cctgacaggc gtagcacgtc agacggtgta acctttgtca tcgacccgcc
     2161 tcttttttaa tcgcttcccg cctgttacac tggatagata accagcattc ggagtcaaca
     2221 gtggtacggc gtttaacttc tccgcggctc gaatttgaag ctgcggcaat ttatgaatat
     2281 cccgaacatt tacgttcatt ccttaatgac ttacccaccc gaccaggggt gtatctgttt
     2341 catggtgaaa gtgacaccat gccgctctat atcggcaaaa gcgttaacat ccgcagccgc
     2401 gtcctttctc atttacgtac cccggatgaa gccgccatgc tacggcaatc ccgacggatc
     2461 agctggatat gtaccgccgg tgaaatcggc gctctgctcc ttgaagcgcg attaatcaaa
     2521 gaacaacagc cgctgtttaa taaacggttg cgccgcaatc gccagctctg tgccctgcaa
     2581 ttaaatgaaa agcgcgtcga tgtggtgtat gccaaagagg tggatttttc acgagccccc
     2641 aacctgtttg gcctgtttgc caataggcgc gcagctttgc aagcattgca gaccatcgct
     2701 gatgaacaaa aactttgtta tggcctgctg ggactggaac cgttaagtcg cggtcgtgca
     2761 tgttttcgtt cagcgctaaa acgttgcgcc ggagcatgct gcggtaaaga gagccatgag
     2821 gaacatgcgc tacgcttgcg ccagtctctg gagcgtttgc gggtggtgtg ttggccttgg
     2881 caaggggcgg tggcgctgaa agaacagcac ccggaaatga ctcaatatca tattattcaa
     2941 aactggctgt ggctgggggc ggttaattcg ctggaagacg cgacaacgtt aattcggaca
     3001 cccgccgggt ttgatcacga cggttataaa attctttgta agccgctgct ttccggtaac
     3061 tatgaaatta ctgaacttga tccggcgaat gaccagcgag ccagttgatt tcactgaaca
     3121 acagtttacc ttgcggttgt aaaagccgca gggtgtgcct gccatcgaac cagcacgcgc
     3181 cctggtcggt agtgagcaat ttatcgccca gttgccacgc cccgttaatc acaaacacca
     3241 ccccgccgcg tgagccaaag gtagtaaacg tacgttcggc aattctgact ttcgccttac
     3301 agacatccag gcgtgtcatg atgttgaaat ccatcgacat ctgcccttcc gtcagtttcg
     3361 cttttaccac ctggtccgct gcaaaggcaa aaggctgtaa cggttttaag gtatggttaa
     3421 agcggtctgc gctttcaagg agcatctcgc cgccttccag caacgtcact atcctttcca
     3481 tgccgggaaa caaagaaaac tctccattcg ccgcgatgga tgcaatgctg gcacgccagt
     3541 aaaaatcacg ttttgccggt gggaaagtgc aaatttcgcg cgtttcaccg gcagcatttc
     3601 gccacagatt caccgacatt ttacgcatat caaagtattc catactcgct cccggcccgt
     3661 tcttttcatt gttatcgttc aggtaaccgt ggatggttat cggcaactcg ggtgatttac
     3721 gttagtggtg atcaggcttt ttttacaact gtttgagaag agaaaagaaa accgccgatc
     3781 ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt tttttggctg
     3841 aaagattatt cagcagttgc aggcatttta ccttttgccg ctggacgttc tgtcagacgc
     3901 ttctcaaaat tagcattaaa ttgctttttc tgttccggcg tcaggatgtt gtaaattttg
     3961 ttctgggttt ccatgtgcgc cagcatgtta gctttgcgct gttcttccat ttttgcgatc
     4021 tgcgcttcag cttttgcttt atcgaaggta tcgctgacaa tgatgtcatg cattgcgcgg
     4081 cgttcttcca gcggcggacg tttcatctgg tcacgctggc ctttcatgat ttcgcggatc
     4141 tgctgtttct gcgcgtcggt caggttcagg tctttgaaca tcatgtcctg atgcggaccg
     4201 aacttgcctt tgtggtgcat catcggcttc gcgtcagccg gtgctgcggc atgggccagg
     4261 ttagccgcgc caagagccag ggtagaggca acaaacagtg cagttaattt acgcatattc
     4321 tatatccttc ctttcagtta tttattacgg ctttcttaag tagcgtgccg tgttgacgag
     4381 attaacttta ctgactttag cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtgta
     4441 aaaaacactt ttgcgccaat tatggagaaa aaaagaaaat ttgatggaga gtgatgagag
     4501 aatattacaa cacaatgatt ttgcagagat tatgaagaac tataccggat gactggtgat
     4561 aaataaagca aataaccagg attaatctgt attaatttat aagaaagcaa cttaataccc
     4621 gcagaatgat ttctgcgggt aagtattagc ttattttttc gagcattaat cccgcgcgta
     4681 atcccaacgc taccaacgga ttagggaata acacatactc tacatcatgg gttacggtaa
     4741 aacgttcctc tccgtcctgc gccagcaatg ttcctttctc aaacggcata aaattcagcg
     4801 tgtcacttgc catatgcatt tcgaaggacg gcgagtggcg agtaatttgc gaaaccaccc
     4861 gataacggag cggcggtgtt ctcacgatac cgacactctc accagatagc agcgcagcaa
     4921 ttgcgctggc agttactgca aactggcgaa gatcgttttg cccaaagggc aacgctttgc
     4981 caagttccag cgtacaggcc agcgcgccaa aatgtctggc gctgaaatgg gtaaacgtac
     5041 caccaggttc ctgatggaac accagcgcct ccagccccgc cgcacccagc cacgtcagaa
     5101 atttctcgtc ccagggaatg tcgcgttgcg gtaatacacc gaactgcgga tgcaaggagc
     5161 cacgaattgc ggtatgtaga tcaaggtgcc agcgcacaga ttctttgccc tggtcataaa
     5221 aatcttccag gcactgttcc agttcgcgcg cccgacaggt ttctccgctt tcagcaaata
     5281 gctgccaacg accgccaaac attcgattca tatcgctatg gcaataacgt ttcccttgct
     5341 tcagcgcagg aggattcccg aggatcacca gcaaccgcca acgtaacggg atctcgccgt
     5401 gagatatcgc gccaagcaac gcgtccagca tttccacagg tgccgtctca ttaccgtgga
     5461 tccccgcaga aatcaccagc gcgccttgcg gtggcgttaa tggcgtcagt tccagcacac
     5521 catcgcccag ccagcgccag cgaacgccgt tgatttctcg ctcggtgata accgtttttt
     5581 tacccgttaa ggtcagagca agaaaattat ccattgcccc ctccctcgcg ctggaacgga
     5641 taaaccgaac cgagattcag taattggctc aatacatcca gtgcttcccg cccttcgcgc
     5701 agcaattgcg ggtcggccag atcggcagca gtaagacgat cgcggtagta acgatccacc
     5761 cagtcattga gcgcattaaa caaggtatcg ttcatcatca ccgccggatt caccgcccgg
     5821 cgttcttctt ctgtcaatac cacccgcaac cgcaggcacg ccgggccgcc gccattcgcc
     5881 atgctttcac gtaagtcaaa gacttttagt tcgctaatcg ggttgtcagc ggcaaggagt
     5941 tcattgagat aaccccatac tccggcgtgt tcccgacact cctgaggcag caccaacatc
     6001 atggaaccat catcgcggct caacagttgg ctgttaaaca gataggtaga caccgcatcc
     6061 gacacggaaa cctgagttgc cggaacttct atcgccataa aaccgttcac ccgcgcccgc
     6121 aggtttgcca gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg
     6181 cggttactta cggcaatcac gtcattatga aaaacaccct ggtcgataac gtccgggttt
     6241 tgctgggcga aaatcacctg ttggggattc acctgattaa gccttgtcac cgcctcgctg
     6301 gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg tatcattgcc ttcttctcgc
     6361 ccgtagacaa aaagttgcat acccggctca ccgtaatgac cgccgagacg attgtggttt
     6421 gccgccccct catcaccgag caacgctacc tgcggcaacg ccgaatggac gctaaatttc
     6481 tcttcgtcgt taaaaatagc ttttaacagc gattcagtga cgggcgcttc cagcgaacgg
     6541 tgaaatttat tgttcaggtt ggcaacggtg agatgcactt tgccatccag cgtatcggca
     6601 gatggcgcga tcgttgccgc attggctacc cacattggcg aagcggagct gacgctggaa
     6661 agccagtgcg gtgcctggcg tgcaactttt tccagtacct gctcatcgct accactgaat
     6721 cccaactgac gcagcaccgg aataaacgga cgctcgtgcg gcgggatcac ggcctggggg
     6781 aatcccgcat cggcaagggt tttcattttc agtaagccct gtttcgccgc cagtcgcggg
     6841 ttagacacct gaaaacggtg acgggtagag gcttcattac caaacgacag gccagcgtaa
     6901 tgatgcgtca gccctaccag cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct
     6961 catcggaaaa atccagcccg gggttaagcg ttgcgggcaa agttaacgag tccgactcca
     7021 ggctcgccat aggccatgtg cagtaatctg cggcatacca ggcgctgggg cgatggttac
     7081 cggatgcacc aatgccgccg aatggcgcgg tactggcagc accagtaagc ggtttgttcc
     7141 agttaacaat ccccgcccgc gcctccagca acagttgatc gaatttttcc cgctcggggg
     7201 aaaccagacc gcaagagagt ccgaagcgag tgttattcgc cattagaatc gcttcctcga
     7261 aagaatcata acgccagacg cgcaataacg gtccgaacac ctcttcatct ggtacgccag
     7321 caacgcctgt catttcaatg atccccggcg tcagcaacga tgtctctgat tgtaataagc
     7381 gcggcgcaag cagggttcgt ccgcccatcg cttccagttg ctgccaggca gtaaccacct
     7441 gctgtgcggc ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt
     7501 tgcccggcgt taatcgctgg ctgacggcaa ccagacgagc aagaaacgca tcgccctgcg
     7561 ctccgctttt gagcaataaa cggcgggcgc aggtgcagcg ttgcccggct gtgacaaacg
     7621 ccgactgaat ggtcagatgg acagccgcgt cgatatccgc cacctcatcg ataattagcg
     7681 gattattacc gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat
     7741 gcagctggta gccggtattg gcgcgaccgg taaacagcaa accgtcgaga tcctccagcg
     7801 cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag gttcagcacg cccggcggca
     7861 agccagcctg ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg
     7921 gtttaaagat aatggtgtta cctgccagca atgccggaac gatatgtccg ttcggcaaat
     7981 gaccagggaa attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc
     8041 tcgccgcacc gtccggcatt tcactacgct gctcgccggt acgaacgtga tacgccttaa
     8101 ttgatatcgc gattttattg atgttgccgc ttcccagcgc ggcttacccg tttctctggc
     8161 aataatcgcg gttaattcgg ctttattgct ttccagcaat ccggcaaagc gttcgacaac
     8221 gacctgacgt tcagccaatg agagccgcgc ccagcgcgga aacgccgcac gggctgcccg
     8281 acaagcctgt tcgacctgag cggcatcggc atcattgcct tgccataaca cctcgcccga
     8341 taccggatta cgcttcacac gcgatgcgcc ctggcccgtt atccagtcac cgttaatcca
     8401 taaagtcatg ctgttttctc cactgcgcac aggcgcacca gacgaacgcg atcccctgcg
     8461 tggcatttga gggcatccag ttgtgcggcg gttaaaatca aacgctcggt tgccggatcg
     8521 gtacgcgcca gcaccacgcg gaaatggtga taattttcat tggcgaccag gcaggccgga
     8581 aaatcgccct gcgcaggctg cccttctgcc acttccacca gccgactttt acggatggcg
     8641 cgcacgcggt cgatgtcaca ctcaagcgtt ggcccgccgt cgaagatgtc gatatagttg
     8701 cggtaacgaa aaccttcttt ctccagcacc gcgcgggcag gcgcggtttg cggatgtacc
     8761 tgaccgatga cgtcctgggc ttcctgggat aaaaagtggg tatagatcgg atgtttcggc
     8821 atcagttctg caataaatgc cttttgcccg gtgccgcaga gaaaatcggc gcggctaaaa
     8881 tccatcgaaa agaagcgttt accgaggctt tgccagaacg gtgaatagcc gtgttcgtca
     8941 atcaccccgc gcatttcagc aaccactttg tcattaaact tgtcgcgaaa agccgccata
     9001 aacataaagc gcgatttcga cagcaaatag ccgttgccct ctttgcgcca gtccgggtcg
     9061 agaaacagcg tgcacagctc gctgctgccg gtgtgatcgt tactaagaaa cagcgtcggc
     9121 aatgcgttat agacattcag ctcttttgag gcgtgaacca acgtgccgac ccgatagttg
     9181 tgccagggat cgttcagccc aaccgccacc tcaatggcgc aaatccccgc cacggtgcct
     9241 gtctcgctat cttccagcac gaacacatag ccctgctcgc ttttgggcag ttcgccttgc
     9301 caggttttga ttgccctttc gatacgcgcg gaaagcgtgg cttcattagc gggaagcgac
     9361 gtcaggccgc cgcccgtttt gctggcaagc tgcatcagcg tcgagacatc tgagcgctca
     9421 acgggacgga tgaccatcat gatgaacctc ggctaacaaa gtgttcgcaa gcagctgcaa
     9481 agcgatccag tccggtcgtc acctcttctt cgctgacatt gagcgcaggc gcaaaacgca
     9541 ccacgttgcc acccgcaatc agtaccataa cgcctgcttt cgccgcctcc tgagagatct
     9601 gtttcgcttg tccggcgtaa tcggcattca gtacacagcc aatcaacaaa cctaagccgc
     9661 gaacttcact gaacaaaccg tagcggtgat taatggtatt aagacgctca acaaaccagt
     9721 cgtgacgctg tttaacgcca ttaagcatct ctggtgtgtt gatgagctcc agcactttgc
     9781 ccgccaccgc cgaggccagc ggattgccgc cataggtggt gccatgagtg ccaacggtca
     9841 tcacgctggc gcactcttcg gttgttaaca acgcaccgac cgggaaatcg ccgcccagcg
     9901 ctttgacggt agttaacaga tcaggtgtca cgccgtagtg catataggca tacagttccc
     9961 cggtgcgccc gacgccggtt tgtacttcat caaaaatcaa cagcgcattg tggcggtcac
    10021 acaattcacg cagaccttgt aaaaacgcgt tgctggctgg caccacaccg ccttccccct
    10081 gaatgggttc gacaatcacc gcacaggtgg cgtcgtcaat cagcgcgctg gcagagttaa
    10141 tatcgttata tgcagcatga cggatatctg gtggcagtgg cgcaaaatcc tgtgaatagg
    10201 ctggctgccc acccgcactg acagtaaaca gcgtgcgacc atgaaacgca tttttaaacg
    10261 ccatgatacc gctcttatgg ctgccgtagc ggtcgtgagc gaatttacgc gccagtttta
    10321 gcgccgcttc gttggcttcc gcaccggagt tacaaaagaa gacgcgatcg gcaaacgtgg
    10381 cgtcgatcaa ttttttcgcc agtcgcagta ccggctcgtt ggtgtaaccg ttgccggtat
    10441 gccagaactt actcgcctgt tcgttcagcg cttcacgcag ttccggatgc gcatggccta
    10501 gcgcgttcac cgcaatgcca cccgcgaagt cgatatactc tttcccctgc tgatcccaca
    10561 agcgcgaacc ttcgccacgt accggtataa agggtgccgg agcgtaaaca ggtatcatcc
    10621 attcatcaaa gttttcacgc gtaattggct gagacatagc gacctctaca gtaaataatt
    10681 cgttatttat atgttgacaa taagtaatgt ttgcgctgta aatgtagatt gcagggttcg
    10741 tgccagccag tgataaaagt gcataaacgg cggaggctaa ctggaaatca aggagttata
    10801 accaaaccat atgcatttaa agtgcatata aagtgaatac gtttgcgatg tgggtgaata
    10861 aaaagaataa aaaacgcaat gttatgcaga agtaaaatat aattctggaa ttgtgatcat
    10921 tgacgaaatt tactggaaat tactgcgcca ttctgacgca gcgcgcacca aaagcgggca
    10981 ttttttgcgc catcgttgac atcattaaca accatcgatc aaatcactta acaacaggcg
    11041 gtaagcaacg cgaaattctg ctaccatcca cgcactcttt atctgaataa atggcagcga
    11101 ctatgaaatt tgtctctttt aatatcaacg gcctgcgcgc cagacctcac cagcttgaag
    11161 ccatcgtcga aaaacaccaa ccggatgtga ttggcctgca ggagacaaaa gttcatgacg
    11221 atatgtttcc gctcgaagag gtggcgaagc tcggctacaa cgtgttttat cacgggcaga
    11281 aaggccatta tggcgtggcg ctgctgacca aagagacgcc gattgccgtg cgtcgcggct
    11341 ttcccggtga cgacgaagag gcgcagcggc ggattattat ggcggaaatc ccctcactgc
    11401 tgggtaatgt caccgtgatc aacggttact tcccgcaggg tgaaagccgc gaccatccga
    11461 taaaattccc ggcaaaagcg cagttttatc agaatctgca aaactacctg gaaaccgaac
    11521 tcaaacgtga taatccggta ctgattatgg gcgatatgaa tatcagccct acagatctgg
    11581 atatcggcat tggcgaagaa aaccgtaagc gctggctgcg taccggtaaa tgctctttcc
    11641 tgccggaaga gcgcgaatgg atggacaggc tgatgagctg ggggttggtc gataccttcc
    11701 gccatgctaa tccgcaaaca gcagatcgtt tctcatggtt tgattaccgc tcaaaaggtt
    11761 ttgacgataa ccgtggtctg cgcatcgacc tgctgctcgc cagccaaccg ctggcagaat
    11821 gttgcgtaga aaccggcatc gactatgaaa ttcgcagcat ggaaaaaccg tccgatcacg
    11881 cccccgtctg ggcgaccttc cgccgctaat ttagcagctc tcctggctca aactgggtca
    11941 ggagaattaa ccttgagaaa aatcaacaaa ctgtcagtaa tgatttgttg cctgccgtcc
    12001 tttgttatac cgtcactgcg tttttagttg tctgaccact tctctattat caagtttgat
    12061 ataggaaact ccacgatgaa tgctgagcgt aaatttcttt tcgcctgtct tatttttgcg
    12121 ctggtcattt acgctatcca cgctttcggt ttattcgatc tgctcaccga tttaccccac
    12181 ttacagacac tcattcgcca gagcggattg ttcggttata gcctctatat tctgttattc
    12241 atcattgcca ccctctttct gttaccagga agcatactgg tgatcgctgg aggaatagtt
    12301 tttggcccgc tcttagggac actactctca ttaattgccg ccacgctggc ctcctcgtgc
    12361 tcattcctgc tggcgcggtg gctgggacgt gatttactgc tgaaatacgt gggccatagc
    12421 cataccttcc aggccattga aaaaggcatt gcgcgtaacg gtattgattt tcttattctg
    12481 acccgcttaa tcccgttgtt tccttacaat attcaaaatt acgcttacgg attaaccacc
    12541 atcgccttct ggccttatac ccttatttcg gcactcacga ccctgcccgg tattgttatt
    12601 tataccgtga tggcaagcga tctcgccaat gaaggcatta cgctgc
//
LOCUS       DAJTNA010000117        12532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTNA010000117 DAJTNA010000000
VERSION     DAJTNA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12532
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.118"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..1328
                     /gene="bcsG"
                     /locus_tag="OO161_002767"
     CDS             <1..1328
                     /gene="bcsG"
                     /locus_tag="OO161_002767"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5463776.1"
                     /translation="IGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFSLW
                     PAGQPTTTVTTTGGNAAATVATTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEAKR
                     KSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATSYS
                     GPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLKEV
                     RENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNTLP
                     LHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGDRM
                     QVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGKIF
                     TEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1415..1522)
                     /locus_tag="OO161_002768"
     CDS             complement(1415..1522)
                     /locus_tag="OO161_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5463777.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            1998..3269
                     /locus_tag="OO161_002769"
     CDS             1998..3269
                     /locus_tag="OO161_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5463778.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKAFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLHGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(3299..4303)
                     /gene="dppF"
                     /locus_tag="OO161_002770"
     CDS             complement(3299..4303)
                     /gene="dppF"
                     /locus_tag="OO161_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5463779.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(4300..5283)
                     /gene="dppD"
                     /locus_tag="OO161_002771"
     CDS             complement(4300..5283)
                     /gene="dppD"
                     /locus_tag="OO161_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5463780.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(5294..6196)
                     /gene="dppC"
                     /locus_tag="OO161_002772"
     CDS             complement(5294..6196)
                     /gene="dppC"
                     /locus_tag="OO161_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5463781.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(6206..7225)
                     /gene="dppB"
                     /locus_tag="OO161_002773"
                     /pseudo
     CDS             complement(6206..7225)
                     /gene="dppB"
                     /locus_tag="OO161_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
     gene            complement(7460..9067)
                     /gene="dppA"
                     /locus_tag="OO161_002774"
                     /pseudo
     CDS             complement(7460..9067)
                     /gene="dppA"
                     /locus_tag="OO161_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
     gene            complement(9812..9888)
                     /locus_tag="OO161_002775"
     tRNA            complement(9812..9888)
                     /locus_tag="OO161_002775"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9852..9854),aa:Pro,seq:cgg)
     gene            complement(9980..11671)
                     /gene="eptB"
                     /locus_tag="OO161_002776"
     CDS             complement(9980..11671)
                     /gene="eptB"
                     /locus_tag="OO161_002776"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5463782.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHILQAKEAAAN"
     gene            complement(11924..12448)
                     /gene="lpfE"
                     /locus_tag="OO161_002777"
     CDS             complement(11924..12448)
                     /gene="lpfE"
                     /locus_tag="OO161_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HCR5463783.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENITPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
BASE COUNT         2998 a         3346 c         3152 g         3036 t
ORIGIN      
        1 tgattggggc catttttgtt ttattagtgg cctggttatt cctgtcacaa tggattcgca
       61 ttaccgtttt tgtggttgcc atactgctat ggctgaacgt acttaccctg gcgggaccaa
      121 gtttctcctt gtggccagcc ggacaaccga cgaccactgt aacaacgacg ggtggtaacg
      181 cagcggcaac cgttgcgacg acgggtggcg caccggtagt gggtgatatg cccgcacaaa
      241 ctacaccgcc aacaacggcg aaccttaacg cctggctgaa taatttctat aacgcggagg
      301 cgaaacgtaa atcgaccttc ccgtcttcgc tgcccgctga tgctcagcca tttgaactac
      361 tggtgattaa catctgttcg ctttcctggt cggatataga agccgccggg ttgatgtcgc
      421 atccactgtg gtcgcatttc gatattgagt tcaagaactt taactccgcc acctcctaca
      481 gtggcccggc ggcgatccgt ttactgcgcg ccagctgcgg gcagacttcg cacactaatc
      541 tgtatcaacc ggcaaataac gactgctatc tgtttgataa cctttcgaaa ctgggcttta
      601 cccagcacct gatgatggga cataacggcc agttcggcgg ttttttgaaa gaagttcgcg
      661 aaaatggcgg catgcagact gaattgatgg atcaaacaaa tctgccggtt attttgctgg
      721 gctttgatgg ttcgccggtt tatgacgata ccgccgtgct taaccgctgg ctggacgtta
      781 ccgaaaaaga taaaaacagc cgtagtgcca cgttctacaa cacgcttcca ctgcatgacg
      841 gcaaccatta tccgggggtc agcaaaacag cggattacaa agcgcgggcg cagaaattct
      901 ttgatgaact ggacgccttc tttactgaac tggagaaatc gggtcgtaaa gtgatggtgg
      961 tcgtggtgcc ggaacacggc ggcgcgctga agggcgacag aatgcaggta tctggcctac
     1021 gtgatatccc tagcccgtct atcacagacg tccccgttgg ggtgaaattc ttcggcatga
     1081 aggcaccaca tcagggggca ccgattgtca tcgaccaacc gagcagcttc ctggctatct
     1141 ccgatctggt ggttcgcgtt cttgatggca agattttcac cgaagacaat gttgactgga
     1201 aaaaactcac cagtgggttg ccacaaacag caccggtctc cgagaactca aatgcagtag
     1261 ttattcaata ccaggataaa ccgtacgttc gcctgaacgg cggcgactgg gtgccttacc
     1321 cgcagtaagc acgaaaaagg ccgcagagtt taacctctgc ggcctgatcc gggcgcaaat
     1381 tgccattacg gcagcctgac gcccgcatga cacgttactt ccgcttattc agccagttca
     1441 cgatcatact ggcaagaatg ccagcaatga ccggagccgc taaatcatgc cagaaggcca
     1501 tgcccagttc tgcgagcgtc atatagccgc ctgtgttgta atgccaacgt ttcgcggcta
     1561 ttcttgagtt gtttgggttc aagattagcc cccgtgatgt tgtcaggtgc atacctgcaa
     1621 cgtgcggggg ttttctctct ccagcaacca atgccaccag ggataaagcc cccgcaacat
     1681 tgcgcctcac cggaaccctt tcggcttgcc gctgatttta cgattgcttt ttactgtcag
     1741 cacccggcaa tcttcctcat tgcgcgatgc cttccagctt aatttcttta tttgcaatag
     1801 ttgcagttca tatccgggct gactccagac tggtagtgaa atgcgcaatt ttgtagaatt
     1861 gcgcctcctc gcgacctccc cagtgaatct gttgcctcat tttttacata ctgcgtattc
     1921 gacttctcca cctgttgcgc aagagaaact gggtttattc atttttgcga ggccgacttc
     1981 tttctggaca ggactttatg cagcacaaca cactatcgaa acacaatcag aaattgccgt
     2041 ttacacgcta cgacttcggc tgggttttat tatgcatagg catggcgatt ggtgccggaa
     2101 ccgtgctgat gccagtacaa attggcttga agggaatttg ggtatttatt accgcagcga
     2161 tcattgctta tcctgccacc tgggtagtgc aggacattta tttaaaaacc ctttctgaaa
     2221 gcgattcctg taatgactac accgatatta tcagtcatta cctggggaag aactggggaa
     2281 ttttcctcgg ggttatctac tttttgatga ttatccacgg gatttttatc tactctctct
     2341 ccgtggtttt cgacagcgcc tcgtacctga aagccttcgg tttaaccgat gccgatcttt
     2401 cacaatctct actttataaa gtcgctattt tcgccgtact ggtagcgatt gcgtctggtg
     2461 gtgaacgatt actgtttaag atttccgggc caatggtggt ggtcaaagta gggattattg
     2521 tcgtgttcgg ttttgcgatg atcccgcact ggaatttcgc caatataacc gccttcccgc
     2581 aagcctccgt ctttttccgc gatgtcctgc ttacaattcc attttgcttc ttttctgcgg
     2641 tatttattca ggtacttaac ccaatgaata ttgcctatcg taaacgggaa gcagataaag
     2701 tactggcaac ccggctcgcg ctgcgtaccc accgaattag ttatatcacg ctcatcgcgg
     2761 tgatcctgtt ttttgccttt tcgtttacct tctcaattag ccacgaagaa gccgtttctg
     2821 cctttgaaca aaatatctcg gcactggcac tggccgcgca ggtgatccct ggacatatca
     2881 ttcatatcac ctctacggtg ctgaatatct ttgccgtact gaccgcattc tttggcattt
     2941 atctcggttt ccacgaggct attaaaggca ttattctcaa tctgttaagc cgaattattg
     3001 ataccaagaa aattaactca cgcatgctga ctctggcgat ctgcgctttt atcgtcatta
     3061 cgttgacgat ttgggtttcg tttcgtgtat cggtgctggt gttctttcag ttgggaagcc
     3121 cgttatatgg cattgtgtcg tgcctcattc cgtttttcct gatctataaa gtcgcacaac
     3181 tggaaaaact tcacggattt aaagcctggc taattctgct ttacggcatt ttgctatgct
     3241 tgtcgccact gttgaagctg attgagtaaa ccggagcgca tggccccggt tttgtgagtt
     3301 aacgctgcgg attttcatcc tgatcaacag caaaacaagc taccagttga ccgccgtagt
     3361 cttttagctg cggctgcaac tgggtgcagg ggccgaagcg ccgacgacag cgggcgttga
     3421 aggcgcaacc cggcggtgga ttcagtgggc ttggtagttc accgctgagc ttgatgcgct
     3481 cgcggcgatc gtccgggttc aggcgcggcg tcgcggaaag tagcgcctga gtgtacggat
     3541 ggcgcgggtt attgaagatt tggtctttcg ttcccttctc cacgcagcgg cccaggtaca
     3601 tcaccatcac ttcatcagca atgtgctcca ccaccgacag gtcgtgggag ataaagacat
     3661 aagacagccc caactcctgc tgcaaatcca tcatcagatt cagcacctgc gcgcgcactg
     3721 aaacatccag cgcggaaacc ggttcatcgg caatcaccac atccgggtcg agcatcagac
     3781 cacgggcgat ggcgatacgc tgacgctgac cgccggagaa catatgcgga tagcggtcat
     3841 agtgctcggt tttcaggccg actttcgcca tcatcgacag ggctttttcc cgacgctgtt
     3901 ctttgcttaa gctggtgttg atcagcagcg gctcttcaag aatttgcccg acttttttac
     3961 gcggattcag cgaaccgtaa gggttctgga agacgatctg gattttctgc cgacgcagct
     4021 tctgcgcctg cggatcgtgc ttaagcagat cctgcccctg gtaatacagc tcgccaccgg
     4081 tgggcgtttc aatcatcgtc agcaaccgac cgagggtcga tttaccgcag ccagattcgc
     4141 ccactactgc cagcgttttg ccacgttcaa ggttaaacga aacgccatcc agcgctttaa
     4201 ccagacgttc cggcgcgaac atgcctttct tcaccggata atgttttttc aggtcgatag
     4261 cctgcaacag cggttgttgc agggtggcct cttgcgtact catagtgtcg gcctcccggc
     4321 atcatcaagt gggtaatggc atttggactg acgcccgtca gcgagcatat tcagcgccgg
     4381 ttcttcagcg cgacatctgt cagtggcata ggggcagcgc gggttaagca ggcagccgtt
     4441 cgggcggtcg tacttgccgg gaacgacacc tggcaacgac gccagacgtt ctttgtcctg
     4501 agcaaattct ggcagcgcac gcagcaatgc ctgagtatac gggtgacgcg gcgcatggaa
     4561 gatggcgtgc gcatcgcccg tttccaccac ctggcctgca tacatcacaa tgattttatg
     4621 cgcagcttcc gccaccagcg ccaggtcatg ggtaatcaac accagcgcca tgttctcttt
     4681 ctgctgtagc tccagcagta gttcgatgat ttgcgcctga atggtcacgt ccagcgcggt
     4741 ggtcggctca tcggcaatca gcagttttgg tcgacaggca atcgccatgg cgatcatcac
     4801 gcgctggctc atgccgccgg aaagctgatg cgggtaaaca tccagacgcg aagccggatc
     4861 gggaataccg acctgattca gcaggtcaat cgcccgctga cggcgggtac ttttgttgcc
     4921 gccctgatgc accttaatcg cttccatgat ctggaaaccc acggtgtagc acgggttaag
     4981 gctggtcatc gggtcctgga agatcatcgc cacttcggca cccaccaggt tgcggcgctc
     5041 tttttctgat atacgctgca aatcctgacc gttaaacacc agtttttccg ccattacgcg
     5101 gcccggataa tcaatcagcc ccataatcgc cagggaactg accgacttac cggagccgga
     5161 ctcacccaca atcccaacca cttcgccctg ttttacgctg tagctgatgc ggtctacggc
     5221 gcggaacggt gcgctttcgt cgccgaaatg caccgataat ttatctacat ttaataacgc
     5281 catctcgaac ctcttactgc ttcagtttgg gatcgagcgc gtcacgcaga ccgtcaccca
     5341 tcaggttaaa tgccagcacc gtcagcagga tcgccagacc cgggaaggtc acgacccacc
     5401 aggcgctttg cgcgaactgc aacacgtcgg agagcatggt gccccactcc ggcgttggcg
     5461 gctgtgcccc catgccgagg aaaccaagag cagccatatc gagaatggcg ttagagaaac
     5521 cgagcgacgc ctgaacaatc agcggcgcaa ggcagttcgg gaagatgtta ataaacatct
     5581 gacgcatcgc cccggcaccc gccacgcgag acgcggtgac gtaatcgcgg ttaacttcca
     5641 ccagcacggc ggcgcgggtt aagcgcacat agtgcggcaa ggcaacgaag gtcagtgcca
     5701 gcgcggcgtt accaatcgac gggccgaaaa ttgccaccag caccagcgcc agcagcagac
     5761 ttggcagcgc cagcatgata tcgaccacgc gcataatgat gttatcgacc aggccgccaa
     5821 agtaaccggc gatcaggccg agaataacgc ccataatcag cgataacaca actaccagac
     5881 agccaaccag cagcgacagg cgcgcaccgt acatcaggcg cgacagcata tcacggccta
     5941 cgtcatcggt gcccagcaag tgcgccatgc tgccgccttc ctgccaggct ggcggggcga
     6001 gcagtgcatc gcggaactgt tccgccgggt tatagggtgc aatccagttg gcaaagatcg
     6061 cgatgaacag cacgatgacg acgtaaacca gcccgacgac cgcgcctttg ttgcgtttaa
     6121 aatagtgcca gaactcctgt aacggggtca tcggcaccgg tgcgctaatc actttatttt
     6181 cagtaacctg tgacatgatg tccccttact tcttatgacg aatacgcggg ttcaccacgc
     6241 cgtacagcag atcgaccagc aagttgacga ggataatcat cgtcgccacc agcaatacgc
     6301 cgccctgcac taccggatag tcgcggcgtt gcagtgcgtc aatcaaccag cgtcccagac
     6361 cgggccacga gaagatggtt tcggtcagaa tcgcccccgc cagcaatgtt cccacctgca
     6421 ggccgataac ggtcaccacc ggcagcatcg cgttacgcag cgcatggacg ataatcaccc
     6481 gcatgcgggt tagccctttg gcgcgcgcgg tgcggatgta atcctcgccc agcacttcca
     6541 gcatcgagga gcgtgtcata cgcacaatga ccgccagcgg aatagtaccc agcacaatgg
     6601 caggcaagat catatgggcg acggcatcga taaagttgcc gtcttcaccc cagatggcgg
     6661 tgtcgattag cataaaaccg gttaacggat tggagtcatc gaggaacacc atatcgctca
     6721 cgcgaccgga gacgggcgtc aggttccagt gcaccgaaac cagcatgatc agcatcatgc
     6781 cccaccagaa gatcggcatc gagtagccgg tcagcgccag gccaactgca gtgtgatcga
     6841 aaatggaacc gcgtttaacc gcagccagca caccaaccgg aataccgact gccgtagcaa
     6901 aaatcatcgc gcagacgcca agttccagcg tggcctgaaa gcgcggcacg aactcttccc
     6961 atactggaat tcggcttttc attgaaatgc ctagatcgcc gtgcattacg ccctaaatgt
     7021 aatggagata ctgctgccac atcggtttat ctaagccgag ttcagccagc agctgcgcgt
     7081 gacgctcagg ggagatccca cgttcgcctg ccatgatcat caccggatcg cccgggatca
     7141 tgtggacaaa ggcaaatgtg agaagggtaa taccgataaa cgtggggatg acgagtccca
     7201 aacgtcggag aataaactgc aacataaccc ggattctcta tagtgacccg cagcctggaa
     7261 ctgcgtgtct gtattgctca caaatcaatt cccgcccttg tcggacagag aatgaagtat
     7321 tgctgccgga tgcggcgtta acgccttatc cggcctacaa aatcgtgcaa attcaataca
     7381 ttgcagaatt ttcgtaggcc tgataagcgg agcacatcag gcagttttgc ctttaccatc
     7441 agtcttgtat ggcttttaat tattcgatag agacattttc gaagtgatgt ttgcctaatg
     7501 gatcaaccac atagcctttg acttctttgc gtaccggttc aaacacggtg gagtgagcga
     7561 tgatcagtgc cggagcctga tcgtgcatca ccacctgcgc ttgcttgtac agttcaacgc
     7621 gtttgttatg gtcgtcggta gcacgcgccg gttgaatcag atcttcaaac ggtttgtagc
     7681 accattttga gtagttagaa ccttgttcag aggcggcgca gctgaacagg gtggcgaaga
     7741 agttatccgg gtccccgtta tcgccagtcc agcccatcat taccgtctga tgttcgccat
     7801 ctttcgcacg cttgaggtac tcaccccatt cgtaggtgac gattttggcc tgcacgccca
     7861 ctttcgccca gtctgcctga atcatctccg ccatgcggcg agcgttcggg ttatacggac
     7921 gttgtaccgg catcgcccac aggtcgatgg agaaaccttt ttccagaccc gcttctttca
     7981 gcaaggcttt cgctttttca ggatcgtagg tgtaatcctg aacgtcgtcg ttatagcccc
     8041 acatggttgg cgggatcagg tttttcgctg atacacccgc gccctgataa accgctttga
     8101 tgatcgcgtc tttgttcacc gcgtaggtca gtgcctggcg aactttcacg tcatccagcg
     8161 gttttttctg cacgttatac gagaggtagc cgacgttcag ccccgacatt tccatcaggt
     8221 tgatggattt atcctgcttc atgcgagcga tatctgccgg gttcgggtac ggcatcacct
     8281 ggcattcatt cttctgcaat ttcgcgtaac gcacggaagc atcaggggta atagagaaga
     8341 ccagcgtatc gatctgcggt ttggtgcccc agtagccatc aaacgctttg tagcggatac
     8401 gggaatcttt ttggtactgc tgtaactgga acggaccggt tccgattggg ttgaggtcca
     8461 gtttttccgg tgtaccggct ttcatcatcg catcagcata ttcttttgac agaatagagg
     8521 cgaagtccat tgccagatca gcgaggaacg gcgcttccgg gcgagtcagt acgaactgaa
     8581 ctgtgttgtc gtccaccttt ttcacttcac tgatcagctc cggcaagccc atgccttcga
     8641 agtattcgta gctgccgcca gagactttat ggtacgggtt ttgcgcgttt ttctgacggt
     8701 cgaacgagaa caccacgtca tcggcgttca gttcacgcgt cggtttgaat tctttattgt
     8761 cgtgccactt cacaccttta cgcagatgga aggtatagat tttaccgtct tagctgactt
     8821 cccacttttc agcgaggccc ggtatcactt cagtggtgcc gattttaaat tcaaccaggc
     8881 ggttataaag cggtacggaa gaggcgtcat aggtggtgcc ggaggtaaac agctgcgggt
     8941 taaacccttc cggagatcct tctgagcaat aaaccagagt tttagcctga acacttgctg
     9001 cgacggtcat agccaccagg ctgagaccaa gcttcagcat ccctgacttt ttcaaggaaa
     9061 tacgcattat tctgctccaa ttgtgatgtt tgttgtttta accctttgca gtgggttgtc
     9121 gctgcctgac cttttttgtt ttttgcccgg tcgggtcaac gttatgaggt ggggatgccg
     9181 tactaattaa catcagtgta acagtgcgga tcctccataa aatgcccctc gtgacctaca
     9241 atctgtcaac agaatgtgaa aacgtcaata cagccaaccg ggatttacac caacggtgag
     9301 aatccacaaa caaagattaa aaaaacttca aacagctatt tgcagcagag aaatttgtgc
     9361 tactccaaca taaccagaac aatcagctta atatttagca acatttggtg attaagtttt
     9421 atgcatttaa tgaaaaaaat ctgaggaaaa ggtggatatc tgagcgatta ataccatgaa
     9481 cgctaaaacg cacagcggaa aatgccagcg ccagccataa gtaacgcaaa aaaagattta
     9541 agcaaatata aaaaaagaca atggtttctg tcacaaatcc gttaatggat agtgagatat
     9601 ggggcgcaaa gttggcagga aaatgtgtgg tgtgttggtc gaataaggcg gtcgcgccgc
     9661 atccgacaat aagtgcggtg ccgttgcctg atgcgacgct ggcgcgtctt atcaggccta
     9721 cgtcgagtcg aacctcccag cccccggagg ttctcatcct catgggcttc aaatgcaaaa
     9781 aagcctgctc gctgagcagg ctttttgaat ttggtcggtg atagaggatt cgaacctccg
     9841 accccttcgt cccgaacgaa gtgcgctacc aggctgcgcc aatcaccgaa tgcggggcgc
     9901 atcttactgc gcagatacgc cctcgtcaat cccttaatag caaaatgcct tttgatcggc
     9961 gagaaagtca gcaggccgct tagttagccg ctgcctcttt tgcctgcagg atgtgacacc
    10021 agttgttgtt ttcgttaatt ccaccatccg gtgaagtata gccaagacaa cccatgatgg
    10081 tatcgtacag ctctacgtga cggcgtggca ctttcatgtc ggcttctttt ttcagctgcg
    10141 caaatgcctg cgcattggcc ggattttcca gatatttatc tgacatccag accatcatcg
    10201 gtacgcggaa ctgctccggc ggtgccaatt cacgcggcgt gccgtgcagg tgctcgcgtt
    10261 cattaattga ctcaccgtgg tcagctgcgt agaacacaat cgctttctta tcgcgaacct
    10321 gatcaatcac gctggagata aagtgatcca cataggtcac cgagttgtca taggagttga
    10381 tcatctgcgc tttggtacag ccgctatcaa caccaataca ttccggcttc cactgcgcga
    10441 agctacgcgg ataacgctgg gtgtagttaa aatgcgaacc tttggtatgc agaatgatca
    10501 gatgcttacc atccgggttg cgccctagcg attgctgcat ttcgtctacc agcaacatat
    10561 catctaccgg cttgccacga ttacgtggct ccgcaccaat ctgctcacga taagcaatgt
    10621 tgtccgccat cgtgttgctg tagaaccaca tctcgctctg catagcgtag aggtcagaac
    10681 tgaatcctaa ctgcttcaga accgcgaaaa tgttctgttc ttttaatgtg cgctgcggat
    10741 tatcttccgc gcccccctga cgtacaaaca tgcaacgcag tgagagtttg gttgcggtat
    10801 cacaggagta accacggaac gccgccagat ttttctcctg ggccagtttc ggcgtggtat
    10861 tacgctcata gccgaaaata cccatatggt cccaacgcgt ggtttcaccg atgataaaca
    10921 cgacataagt atcatcaacg ttttgtggag cctggtatgt aaacttcttc gccggattaa
    10981 gcaatgaatt attatcggaa gattcatcga cccgcgccca ggcatacagc cccaacgcag
    11041 aaagccagtt tgatggcaga taagagttcg ctactacacc gccataactc ggcaaatcaa
    11101 cgccggtcgc cctctccact ttcttctgct ggatatccag caaacgaatc ggtgcccaaa
    11161 ccataatacc cgccagtacg acgaccgcca ggctgcgaat acgctgccct ggggttcgca
    11221 gttgtcggag caaggtgtag cgacagcggt tattccagat aaggataaga ggcaatgcac
    11281 taacggcgat taaccagaga ataaagttca gaccaacaac ttcttttgac aggtcgatat
    11341 cggtggtcat gacggaagcg atgatgccat aaccaatgac cacattaagg aaggtcatgt
    11401 aatagctggc acctgcggaa aagagcacca ccagcgacgc cagaatacgc cagctgcggc
    11461 ggccaaacag cgatagaaga cgtagtaaaa agaaggtcac cagtacggtg gcggccagtt
    11521 caacaacagc agaaacgcct ttccagacgg taaaatcgtg cgcatagctg ccgaagcggc
    11581 gataaaaaac cgcgccattc ataaaaagac caatatagat tgcaagcaaa aagctcagct
    11641 tctgctgtgt aatcgatttg atgtatctca tgcaaacaaa ccctggacaa acaggtgata
    11701 acaccctgct tagcggcttt tcaggcccgg caggggaaaa gtgcggaggt agtcagataa
    11761 gtaaaatcta cacgcgcgaa taagtagacc acagcaaagc gaaaaagtgt ggggaaagag
    11821 tgtgcatgaa gcacaaattt acaaaaatgc aagtttgaaa agagacttta gcgttacaaa
    11881 aagcagtaaa aagggccagt taactggccc ttaagatgtg aaattattcg tactgtacag
    11941 taaagtcgac cgttgcatct gcgcgaccaa tagttgcatc ggcgcgaata ggcacgtagc
    12001 tggcgatata gttcagttga tgtgcgccat caccgttaat tgcaaactca accggtgtcg
    12061 gcgttatatt ttccgggaac tgttgtactc cagcagcggt tttgattttc agcccaacgc
    12121 ctgtagcgcc acccgcatcc agcgcaaaaa gatttgagtc gtctgcattg ggtgtaccgt
    12181 ccatagtcac gtaggctctg accacttcgc tacagttttt caggccgatg gaaaaaggag
    12241 tgggattaat ttccgttccg attcttaccg gctttttgag aaccgcggtg cccatgtcaa
    12301 cctcaacgct gtctttcacc agttcgcaag caccctggct gattttaagg gtgaatttca
    12361 atgttccttt cggccctaaa tccgtggttg ccgcgaatga cgaaaaggaa agaggcagca
    12421 ggcataacgc aagtattgtt cgcttcatga ctgttactcc agttcgacat gtatggtagc
    12481 aataccgcta taatccccgg cagcgggtaa ttttccggta gtgctaacgg ga
//
LOCUS       DAJTNA010000118        12455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTNA010000118 DAJTNA010000000
VERSION     DAJTNA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12455
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.119"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            470..1294
                     /gene="gadX"
                     /locus_tag="OO161_002778"
     CDS             470..1294
                     /gene="gadX"
                     /locus_tag="OO161_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5463784.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKFSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFLIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1663..2391
                     /gene="gadW"
                     /locus_tag="OO161_002779"
     CDS             1663..2391
                     /gene="gadW"
                     /locus_tag="OO161_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5463785.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2754..5867)
                     /locus_tag="OO161_002780"
     CDS             complement(2754..5867)
                     /locus_tag="OO161_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5463786.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5892..7049)
                     /gene="mdtE"
                     /locus_tag="OO161_002781"
     CDS             complement(5892..7049)
                     /gene="mdtE"
                     /locus_tag="OO161_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR5463787.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7109..7387
                     /locus_tag="OO161_002782"
     CDS             7109..7387
                     /locus_tag="OO161_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463788.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7388..7915)
                     /gene="gadE"
                     /locus_tag="OO161_002783"
     CDS             complement(7388..7915)
                     /gene="gadE"
                     /locus_tag="OO161_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5463789.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHVPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8714..9286)
                     /gene="hdeD"
                     /locus_tag="OO161_002784"
     CDS             complement(8714..9286)
                     /gene="hdeD"
                     /locus_tag="OO161_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5463790.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9541..9873
                     /gene="hdeA"
                     /locus_tag="OO161_002785"
     CDS             9541..9873
                     /gene="hdeA"
                     /locus_tag="OO161_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5463791.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQVCTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9989..10315
                     /gene="hdeB"
                     /locus_tag="OO161_002786"
     CDS             9989..10315
                     /gene="hdeB"
                     /locus_tag="OO161_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5463792.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10379..11026
                     /locus_tag="OO161_002787"
     CDS             10379..11026
                     /locus_tag="OO161_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR5463793.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     MTAAIGMVIGSGMYELGMYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11068..11598)
                     /locus_tag="OO161_002788"
     CDS             complement(11068..11598)
                     /locus_tag="OO161_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5463794.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11754..12320)
                     /gene="slp"
                     /locus_tag="OO161_002789"
     CDS             complement(11754..12320)
                     /gene="slp"
                     /locus_tag="OO161_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5463795.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3441 a         2768 c         2824 g         3422 t
ORIGIN      
        1 tgaactgttg ctggaagact acaaagcctc cctgaaatat ctcagcgatc acccgaaact
       61 gcagggtatt gcccagcaga acagctttaa acacacctga taacataacg ttgtaaaaac
      121 cgaatgccca gcctttaaaa aaacagctgg gcattcggtt gcttattaat cgcaataata
      181 tattggctgt ctattcatcg tgttgataag atatatgtta tatgaatgtt tattaaatgt
      241 agcgattgcc tttaatgtca cgaaagggga ttattttctt gctattaatt tcactgcgtt
      301 cttaaacacg tttttgtatt ataaatccag tcatcctgcc cgtagaacat cacacattat
      361 catcctgttc tcccgcttcg tttaaattta tttatcaatc aatttgactt aagagggcgg
      421 cgtgctacat taattaacag taatatgttt atgtaatatt aagtcaacta tgcaatcatt
      481 acatgggaat tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg
      541 tgaatatcgc tattttaatg gtggtgacct ggtttttgcg gatgcaagcc aaattcgagt
      601 agataagtgt gttgaaaatt ttgtattagt atcaagggat acgctttcat tatttctgcc
      661 gatgctcaag gaggaggcat taaatcttca tgcacataaa aaattttctt cattactcgt
      721 tcatcactgt agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa
      781 gaatcttcgc tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt
      841 ttttcttgag gatgagcact ttataccgct gcttttgaac gttttgcaac cgaacatgcg
      901 aacaagagtt tgtacggtta tcaataataa tatcgcccat gagtggacac tagcccgaat
      961 cgccagcgag ctgttgatga gtccaagcct gttaaagaaa aaattgcgcg aagaagagac
     1021 atcatattca cagttgctta ctgagtgtag aatgcaacgt gctttgcaac ttattgttat
     1081 acatggcttt ttaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt
     1141 catttacgtc tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc
     1201 gcagggattg ccgaaccgtg actcggcggc aagtattgtt gcgcaaggaa atttttacgg
     1261 cactgaccgt tctgcggaag gaataagatt atagaatttt actcagacat aaaaaaaaac
     1321 ccggcatagg ggaccgggaa gaggatagtc tgccgtctcc agactaataa accgttataa
     1381 cactccctgt tggcacggga aactttgtgc tctcagtaag ttaaatataa cttttactgg
     1441 aaataagatc agccattttt ttataaacat aagctatacg ctgtgcgaaa atatattcgt
     1501 gctgcattta cttattatca attaactgtt atgcaaaact actttgtgga taaattttgg
     1561 tcctaccaaa tctggcagtt tttgcgctaa gaaacagtct ggcaacattt cattagtata
     1621 ctgaaattga aataatcgca gtatgaaata taagggataa tcatgactca tgtctgctcg
     1681 gtgatcctca ttcgtcgttc attcgatatt tatcatgaac agcataaaat atcgctgcat
     1741 aacgagagta tcgtgctgct ggagaaaaat ttggcagacg attttgcgtt ttgttcaccg
     1801 gatacgcgac gactggatat cgatgagctg acagtttgcc attacttaca aaatattcgt
     1861 cagctaccac gcaatttagg gttacatagc aaagaccgtc tattaattaa ccagtcaccc
     1921 cccatgccgc tggtgacggc gatttttgat agcttcaatg aatccggggt aaattcaccg
     1981 atactgagca atatgctcta cctttcctgt ttatcgatgt tttctcataa gaaagaactg
     2041 atccccttac ttttcaatag catcagcact gtttcaggaa aagttgaacg ccttattagc
     2101 tttgatatcg ccaaacgttg gtatctgcgc gatatcgcgg aaagaatgta taccagcgag
     2161 agtctcatca aaaaaaagtt gcaggatgaa aatacctgtt tcagtaaaat attactcgct
     2221 tccaggatgt cgatggccag acgattactc gagttacgtc aaattcctct gcatactatt
     2281 gcagaaaaat gtggctatag cagtacgtcg tactttataa acacatttcg acaatattat
     2341 ggtgtaacgc cacatcagtt tgcgcaacat tcgccaggta ccttttcctg acatattttg
     2401 catttgaata ttggtcagga tctcacacct gcttcatgtg aaactcttcc ctgatgattt
     2461 ctgccgggct accggctagt tctctttcgc agtttgccag gcggtatagg tattttcagg
     2521 aggaaaagac tcggcatgtt tgggattatt aagctgacaa ttcatacgat tacgggaatg
     2581 tgggtcagta ttgtgctgtt caaattgatg acgaatggct ggagtggttt ctactttcag
     2641 tgctgtgtac taagcctggt attcctgacg gttagctggt tgttgagtgg tgaatggtta
     2701 gcaggaaaga gtaaggctga accttcacgt tcaaccttac tctcatttac acgttacgct
     2761 tttttaaagc gggcaaagag atgttccact acaacgaaaa agaccggaac gaagtaaatt
     2821 gccagcactg ttgcggcaaa catcccgccc atcacgccgg tacctaccgc gttttgcgcg
     2881 ccagaaccgg caccatgact gataaccagc ggcagcacgc cgagaataaa ggccagagag
     2941 gtcatcagga ttgggcgtaa acgcatccgc gccgcttcga tgattgcccc tatcggcgtt
     3001 ttcccttctt tctgcatcat ctcaacggca aattcgacaa tcaggatggc gttcttggcg
     3061 gaaagcccga tggtggtcag caaaccaacc tggaagtaga cgtcattact taagccgcgc
     3121 agatcggtgg ccagtaatgc gccaacgacg cctaacggaa caaccaacat caccgagaac
     3181 ggaattgacc agctctcata gagtgcggcg agggcgagga acaccacgac cagtgaaatg
     3241 gcatacagtg caggagcctg gtttgaggat aacgcttcct gatacgacag tccggtccat
     3301 gagtagccga cgcctgccgg aagtttagcg accaggtctg ccataaattt catagcgtca
     3361 ccggtacttt tcccggccgc cgcttcacct aaaatctcca ttgacgggat gccgttgtag
     3421 cgttccaatc gtggtgaacc ataggtccat tcggtagacg agtaggcaga aagcggtgcc
     3481 atcgtgccag aggcgttgcg tacataccat tggttgatgt tatccggcaa catacggaac
     3541 ggcgtgcctg cctggacata cacttttttc acccgcccct ggttgaggaa gtcgttcacg
     3601 tagctgctgc cgaaggcggt ggaaattgtc tggttgatat cagacagcgc cacacccatt
     3661 gcctcagctt tcgcggcgtt gacgttcact ttgaacatcg gcgtatcttc caggccgttc
     3721 gggcgtaccc cggtgacctg gtcaggcgat tgcgctgcca gtgataacag ctcgtttcgt
     3781 gcctgggtta gtttttcgtg ccccaggtta ccgttgtcca gcagttccat atcaaaacct
     3841 gacgcggtac ccagttcagc caccgcgggt aagttgaacg ggaagacgac ggctttattg
     3901 atactgctta acgcaatcat tgcccgctga atgatcgcgg taaccgagtt ttcctcaccg
     3961 acacgttcag accacggctt gagactgata aacgccaggc cgttgttttg cccctggccg
     4021 ctgaagccaa agccgccaac ggtaaacacc gactgaacat tatctttctc ttttgtaaga
     4081 taataatccg tcacctgttg cagcactttc gtggtgttaa ccatggtggc accggaaggt
     4141 aactgcgcgg tggtcataaa taccccctga tcctcttctg gtaagaaaga ggtcggtgta
     4201 cgcaggaaca gcaccgccat cccggcgcaa atcagcaggt agaccaccat gtagcgaccg
     4261 gtacaacgca acagcgagcg ggtgctatcg gtatagtgct gagttgattt ttcaaacagc
     4321 gtgttgaagc gtgcgaacag ggcgttaggt ttgtgaccgc cttccggcgc ggctttcaga
     4381 atggtggcgc acagggcagg ggtcaggctc attgccacaa atactgaaag cagcatggag
     4441 gagatcagcg tgatggagaa ctggcggtag atctccccgg ttgcgccgct cataaaggcc
     4501 atcggcataa acactgcgga aagaacaaca gcaataccga ccagcgcacg ttggatctgc
     4561 cccatcgatt tatgcgtcgc ttccttcggt ggtagcttat cttccgcaat gacacgctcg
     4621 acgttctcca ccaccacgat ggcgtcatcc accagtaacc ctatcgccag caccatcccg
     4681 aacatagtta acgtgttgat ggtgaaaccg accgccgaca agatcgcaaa cgtcccgaga
     4741 ataaccaccg gtacggcaat cgtcgggatg attgtggcac ggaaattctg caaaaacaga
     4801 tacatgacca ggaagacgag gatgatagcc tcaaccagtg ttttaaaaac ttcctgaata
     4861 gaaatttcga taaacggcgt ggtgtcgtaa ggataaaccg tcttcagact tgcggggaaa
     4921 taggctgata agcggttcag ctcctctttg actgcccgcg aggtatccag ggcgtttgct
     4981 ccggtagcca gtttgatggc gatcccggca gcaggtttgc cgttatagcg cgccacggtg
     5041 gaataatctt ccgctccaag ttcgacgcgg gcgacatcac gcagcagcac ttgcgaacca
     5101 tcttgctgaa ctttcaacag gattttgcca aattcttccg gcgtttgcag acgcgtctgc
     5161 acaatgatcg aggcgtttag ttgctggtct gccgcctgtg gcatgccacc cagttgacca
     5221 ccggaaatct ggttgttttg caccttaatc tgggaaataa catcggaagg taccagattg
     5281 tatttattga gtttttgcgg gtccagccag atacgcatgg cgtactcgga accaaagagc
     5341 tgtacgctac caacgcccgc ggtacggctt agcgggtctt tgatattaga cgctacatag
     5401 tccgcgatat cgtactggtt gaggctgccg ttatcagaaa taaacgccgc taccatcaga
     5461 atattactgc tcgacttatc aacgctaatc ccctgctgct gcaccgcttc aggtaatgaa
     5521 ggcatagcga gttgcagttt attttgcacc tgaacctgtg cgatatcagg agatgtccca
     5581 gtctcgaagg tcagagtgat agaggcatta cccgccgcat cactggttga agacatgtac
     5641 atcaggccat caagcccatt catattttgc tcaatcacct gagtgaccga gtcttctacc
     5701 gtttgcgcat cggcacctgg ataggtagcg ctgacggtaa tggtcggtgg cgcaatctgc
     5761 ggatactgcg caaccggtaa gttcatgatc gccagaccac ctgcaagcat cataataatg
     5821 gcaagtaccc aggcaaaaac cgggcgatca ataaaatagt tagccatgaa agtcccctta
     5881 agcctgcaac gttattgttt cgattcggtg ctggcgtttt cctggctgga ggaaattgct
     5941 cgtgctttga tacccggacg aatgcgttgc aaaccggaaa cgatcacccg atcgccagcc
     6001 tgcaagccag aggtgacaac ccactggtcg ccgatggctt tgctggcttc aatttcgcgt
     6061 agctgcacaa catcgtcttt atccagaatg agcgccgttg ctttaccctg ggcgttgtgg
     6121 gtgacgcctt cctgcggcac cagtaataca ttctggcggc taccttcatc cactaatgcc
     6181 gtgacgtaca tgccaggcag caagtcacca tttgggttgg ggaaaatcgc ccgtaacgtc
     6241 acggagcccg tggtttcatc caccgtcggg tcggagaatt tcagtgtgcc ggtctggctg
     6301 tagcgtttac cattttccag attgagctgt actggcgtac tgccctgaac ctgtttgatt
     6361 tgcccactgg cgacctcttc tttcatgcgc aagaaatcct gcaccgactg cgtgagatcg
     6421 acataaatcg ggtccagacg ttgtacggta accagcgaat ctgcctgatt agcggtaacg
     6481 agtgcgccga cggtcaccga cgatttcccg ctgacgcccg taatcggcga ggtgacattc
     6541 gcgtattgca gattgatcgt cgcctgttca acagccgctt tggcgacggt gacattggct
     6601 tctgcttcat tcagctgggt gcgcgcggtg tcgtaatcct gacggctaac gtagttggtc
     6661 ttcagcaacg atgcctggcg gttaaaggtg atgcgggcat tgctggcggt agagagcgct
     6721 ttcgccagcg agcctttggc ggagtttagc tcggcctgta aaggtgcagg atcaatctga
     6781 tacagcgaat cgccctggtt cactttatcg ccttcgataa agttgcgttt aatgataata
     6841 ccgcccacct ggggacgtat ctcggcaact tcataaggaa cggttctacc gggcaattcg
     6901 ctcaacacat tgaccgaacc gggggagagt gtgacgacac cgacctcagg cgtcatggcg
     6961 gcggcgtttt ccgccgattt gtcatcgcag gcggtgagca tcgcgccgca gaataacaac
     7021 ggtattaaca gctttcttct tctgttcatt ttagtccctg aaaattcttg agaaataacg
     7081 gttctttaaa ttgcgcgcga ggagagatat gagggattta cagaccagcg gcatagtggg
     7141 gttgtcagcc agtaaggtgg gatacaggca cagtgatcga catggtgagg tcaacgacaa
     7201 aaaaaatgtt gccgttctgc caacagttcc tgccagcatt cgggcaacga agggttctac
     7261 tggtggatac acataccagg ggaataaagt cgttttagcc tctcctttag taatcaccgg
     7321 gggtaatgaa atcagcatct gcatccctcg tcatgccagc catcaatttc agttgcttat
     7381 gtcctgacta aaaataagat gtgataccca gggtgacgat gtcgctcata cgtcttagtt
     7441 tcaacttgta cataatgttg aatttctgaa tctttaatgt ttgttgcgaa atattgagcg
     7501 tcaacgtgat attgctttca tttttatttt tcagaatcag ttgcataatt ttgaactgat
     7561 tatgtgaaaa acataattgt gaattcttat ggggcacgtg tttaccataa gtaatctcaa
     7621 gaagattttt gatactttct ttgcggctaa caaaataaat aggcgcttta gcttttagtt
     7681 tactgatgtg ataaggtgcc aaaacaataa ttctctcggc atctaatttc tccagaaatt
     7741 taatcgcttc ttcatcaagg atatgattgt gataggtatc aatgataacc ttgaagggtt
     7801 tattgcctac ttttttgatc tctgacaggg aattgatttt tatcatttcg tgattatctt
     7861 tcaactgcca aaagccctgt aaaagaaaag aatctttcgt catgagaaaa atcataactt
     7921 gctccttagc cgttatcgtt tcgaacatgt catccttttc attatttaca tccttgtccg
     7981 aatcgttgtt caatatagta aacgcctata agaactttac ccttcgtgaa ctaatacgtc
     8041 tattaacact aatcaatact tcagatttat ccatcacgca ctccatatat tgatcctaag
     8101 taagattcct ggttgttatc agcttgtaaa tagtaaacaa aaagttaacg tttgcggatt
     8161 actgcccaag aataagttat caataaccag tttgtgatct ctgaagaata ttactaaagt
     8221 taaaatgtaa tccgatttaa atatcgagtc tccttgtttc gacttaagct ggcaattgga
     8281 ttgccagctt tcttttaacg cattgaaatt acctgagtaa ataacgtatt catgtattca
     8341 ggtttttata tagcgtctgg agacacggat atttatgcaa tgtaatcaac gccagttgca
     8401 aaaaaatgaa cccggattac ttatttaacc gcccatagca tcaacgctta cggaactctt
     8461 tgttcacttt cccacagcac ttttaaaaca ttaaacctga ctacggaaaa tatcagccat
     8521 gataatgttt gaaatggcta atttgccatg gagtgaaaaa aattagatga aattcagtag
     8581 gttgaaataa tcactagcag gtaattattt caatgatagt gcgcaattga tctacaacac
     8641 tgcgtagcgg agagagtatt aatcggatca tagtcacatc aagtgactat gatccgggtg
     8701 acaaccgggg taattattgc tgcttaacga acaaactggc gaagctgaac aggctggcgg
     8761 cgctaaatat cagttcaatt cccaccagtg tggaaaccag cgttacagac accatcggcg
     8821 ttgcaccaag gaatatccag gcaatgacga tatccagcac accaataacg agctgtagcc
     8881 agctgccttt cattgaacgc tgacgatacc aactcatcag gcgaataacc cctgcaacac
     8941 agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc cagctccggt gcgcggatga
     9001 agaaatagcc gatcaataaa taggcgactg cgacgaggaa accggataat accggccaga
     9061 aattatgact gcggttgctg aataacccga caataagcgc aatacccgag cagattaata
     9121 atgcacccac tactgtgctt aaaatatcgc cagagacgaa cgggaaactg atacacagca
     9181 acccgacgat aaacagcagc acggcaataa actggattgc tctgcgatgt tttttaagca
     9241 tctccagatc aaacttcaaa attgttgcct tatctatata taacatagaa ccaccctata
     9301 aaattaagaa gaaaatcccc tgctatcaat ctatgccaaa aacgcgtcta agaatgcagt
     9361 cgatttaata aaaatttcct aattgcagta tctgatgcat ctgtaactca ttgtattgaa
     9421 ataaaaatat ctgattttga tattttccat caacatgaca tatacagaaa accaggttat
     9481 aacctcagtg tcgaaattga ttcgtgacgg ctctttcact ttatagttga ggatattacg
     9541 atgaaaaaag tattaggcgt tattcttggt ggtctgcttc ttctgccagt tgtgagcaat
     9601 gcagcggatg cgcaaaaagc agctgataac aaaaaaccgg tcaactcctg gacctgtgaa
     9661 gatttcctgg ctgtggacga atccttccag ccaactgcag ttggttttgc tgaagcgctg
     9721 aacaacaaag ataaaccaga agatgcggtt ttagatgttc agggtattgc aaccgtaacc
     9781 ccagctatcg ttcaggtttg tactcaggat aaacaagcca actttaaaga taaagttaaa
     9841 ggcgaatggg acaaaattaa gaaagatatg taattccggg aatgcgttac atcgtacttc
     9901 cttgcatatt gaacaggccg gaatatcttc tttaaaagca gctattcctc ctgttcatat
     9961 ataatctata tattgaatgg gttacaaaat gaatatttca tctctccgta aagcgtttat
    10021 ttttatgggc gctgtagcgg ctttgtcact ggtgaacgca caatctgcgc tggcagccaa
    10081 tgaatccgct aaagatatga cctgccagga atttattgat ctgaatccaa aagcaatgac
    10141 cccggttgca tggtggatgc tgcatgaaga aacagtatat aaaggtggcg ataccgttac
    10201 tttaaatgaa accgatctca ctcaaattcc taaagtgatc gaatactgta agaaaaaccc
    10261 gcagaaaaat ttgtatacct tcaaaaatca agcatctaat gacttgccga attaatgagg
    10321 tacaagtaaa aaggagtagc aagttgagcc atcttgctgc tcctttttgc atttttatat
    10381 gacagcagaa tttattatac gtcttatact cgcggcaatt gcctgtggcg ctattggcat
    10441 ggaaaggcaa atgcgaggca aaggagcagg gttacgcaca catgtattaa ttggcatggg
    10501 aagcgccctg tttatgattg tttcgaaata tggttttgct gacgtgctgt ctttagatca
    10561 cgtcggactc gaccccagcc gtattgctgc tcaggtggtg acgggcgtcg ggtttatcgg
    10621 tgcaggtaac attctcgttc gtaaccaaaa tattgtaggt ttgacgacgg cagcggatat
    10681 ctggatgacc gccgctatag gtatggttat tggcagtggt atgtacgaat tgggtatgta
    10741 tggttcagtg atgaccttgc tggtgctgga agtcttccat caattaacct tccgcctgat
    10801 gaacaaaaat taccatttac agctgacatt agtgaacggt aatacggtat caatgctcga
    10861 ctggtttaag cagcaaaaaa taaaaacgga tttagtttcg ttgcaggaaa atgaagatca
    10921 tgaagtggtc gccattgata tccagcttca tgccacaacg tcgatagaag atctgttgcg
    10981 attattaaaa ggtatggcag gggtgaaagg cgtttcaatt agctaatctg tgtggcagcg
    11041 tagtcagact caccgtaagc ctgaaattca caccagataa tcaatatgct gatggcgtac
    11101 cagctcattg atccgcttaa gattgaggat catcatgata ttgtgtttat ggcagtagaa
    11161 cgtttttagc gttattttca gtttctccag aatatcctcc gttgtcattt gattcgcaat
    11221 gcagctcaga acctgatgtt gcttgcgcga aagtgacaca gccgcttcag gcacagagca
    11281 gtaactgcca ttgatgagtg cgacaaaatc gatgatagtg atagtccgcg gaacaaaggt
    11341 caccggcact ttccccaggc agcgtttaat attaaagggg gagaaaataa tcacatggac
    11401 aggttttaat cgttcaattt gagtgagaaa attagaatga aatacattat tcattaatgt
    11461 atcaataatg acaaaggcat tctgatgcaa cattccgtcg atctcttctt cgttctgtat
    11521 atgaacgaca ttacctttgc tcaaaatgtt tttcatcgcg gtgaagaaca tcgtatccct
    11581 ggtaattata agaaacataa ccgactcctg gtgcggacat taattttata atcagtttgc
    11641 caaaggtaat taacaggaca gggcttataa taataatagt tagcttgctg tgtattaatt
    11701 ttatgtaatg cacgatgcag atgctgagaa agcatctgca tctttcggtg gtgttatttg
    11761 accagctcag gtgttacctg actcaccgca ttggtgtagt aaggcgcacc ccagcccggt
    11821 tccggccaga atgcaccata gccgtaatcc cataggttat aggtggtatt aaccacttct
    11881 ctcaaatgcc acacctggat gccctgcata ttcacttcca ggaagttata cgggacttta
    11941 ttgataaagc caggttgttc accctgaatg gtaccgagga tggtaacaaa gtgattacga
    12001 tagttcactg ggtcaaggaa gccgctttgt ctggcgagca gtcggccctg atagttggct
    12061 tcaatatcag gtttcgcata gctatccaac ggtaatacag cgatttctaa caacgtatcc
    12121 gttttgccat tgataacgtt gataaccttc ccaccaaagc gcgcttgttg accaacatat
    12181 aaccccggct ggttatgaac agcaacaaaa cttttttgaa tatcaggttg gttattgcct
    12241 ttgatatttt gcggaattga actacatgcg gcaagcaaaa atgaaaggct gaggatgagt
    12301 gcaccttttg tcatgttcat gttactatcc ttatcaacaa atattaaaac cataatagat
    12361 atttctttat agttttcatc tgattctgag gcgatatgcc tgatagtaac gtaaacctgt
    12421 ctgatgttta tatccctttt gtttagttca attcc
//
LOCUS       DAJTNA010000119        12410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTNA010000119 DAJTNA010000000
VERSION     DAJTNA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12410
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.120"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..336)
                     /locus_tag="OO161_002790"
     CDS             complement(<1..336)
                     /locus_tag="OO161_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HCR5463796.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(555..1036)
                     /locus_tag="OO161_002791"
                     /pseudo
     CDS             complement(555..1036)
                     /locus_tag="OO161_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1157..1429
                     /gene="rcnR"
                     /locus_tag="OO161_002792"
     CDS             1157..1429
                     /gene="rcnR"
                     /locus_tag="OO161_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5463797.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      1521..1617
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1652..2440
                     /gene="thiM"
                     /locus_tag="OO161_002793"
     CDS             1652..2440
                     /gene="thiM"
                     /locus_tag="OO161_002793"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5463798.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            2437..3237
                     /gene="thiD"
                     /locus_tag="OO161_002794"
     CDS             2437..3237
                     /gene="thiD"
                     /locus_tag="OO161_002794"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5463799.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            3302..4120
                     /locus_tag="OO161_002795"
     CDS             3302..4120
                     /locus_tag="OO161_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5463800.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            4172..4918
                     /locus_tag="OO161_002796"
     CDS             4172..4918
                     /locus_tag="OO161_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5463801.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(4892..5760)
                     /locus_tag="OO161_002797"
                     /pseudo
     CDS             complement(4892..5760)
                     /locus_tag="OO161_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(5757..6761)
                     /locus_tag="OO161_002798"
     CDS             complement(5757..6761)
                     /locus_tag="OO161_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR5463802.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVNSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPPLKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(6758..8035)
                     /locus_tag="OO161_002799"
     CDS             complement(6758..8035)
                     /locus_tag="OO161_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HCR5463803.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            8292..9344
                     /gene="fbaB"
                     /locus_tag="OO161_002800"
     CDS             8292..9344
                     /gene="fbaB"
                     /locus_tag="OO161_002800"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5463804.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAYRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9652..10506
                     /gene="gatY"
                     /locus_tag="OO161_002801"
     CDS             9652..10506
                     /gene="gatY"
                     /locus_tag="OO161_002801"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5463805.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQINEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            10535..11797
                     /gene="gatZ"
                     /locus_tag="OO161_002802"
                     /pseudo
     CDS             10535..11797
                     /gene="gatZ"
                     /locus_tag="OO161_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            11807..12259
                     /gene="gatA"
                     /locus_tag="OO161_002803"
     CDS             11807..12259
                     /gene="gatA"
                     /locus_tag="OO161_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5463806.1"
                     /translation="MTNLFVRSGIYFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            12290..>12410
                     /locus_tag="OO161_002804"
     CDS             12290..>12410
                     /locus_tag="OO161_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5463807.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCR"
BASE COUNT         3180 a         3099 c         3203 g         2928 t
ORIGIN      
        1 gcgatgataa aaaatctcac cgtcgtaggc tttaatgatt ttaccgtcgg tgtcgctgat
       61 caacacgtac gcgccaccca tataggtcca gtgcgtcccg gcatcaggcg cgggcaggtt
      121 acgtaactgc cactgcttaa tgttgtactc atcggtgcgg tacatctctg ggacggtatc
      181 gcctggtttg aaatgcttaa agtcagcaat gaaactgctt aactcatact tagaaattcc
      241 cgaggtattg gtcgaacccg cggtggctgg tgcggcccag gcggcactgg taaccagcaa
      301 aagcgcaccc agcaacatct tatttttaat ggtcataaac tctccacttt ggggatctgt
      361 aatatcagtc gcatatcttt cctgccatac aatataactg acaagcgata ttttgttagt
      421 gatcccatga tttcgtaaaa agtgtaaatg aaaggatgcc ctaaaagcat ccttagcgag
      481 cagagagtca gcggaaatag ctgatttaag aatcatcctg ggctgaagat aacatgcaag
      541 cctaaaggat tgagttatcg cattatgccc atgaagccat gtacgcccat atacacaccg
      601 actaagccaa tcaacagact ggaaaaatag ggggcgcgtt tagcgagagt gttaaatccg
      661 ctccagcgtt ttgcgacctg ctgaacgctg attgctgcgc caacgcctac ggtgacaagc
      721 gttaacgcca ggccaatgct gaaactgacg accagtgttg cgcccagtgt cagggctttc
      781 aactgaatgc atatcagcgc ttactgatca ccatcccgcc aaaggcaatc aaccagacca
      841 ccgcggtatg cgaaatggtt gctgccagtc cgagcatcac cgcctgttta atggtgcctt
      901 tgatggcgat gataaacgcc gccatcatcg tttttgagtg ccctggttcc aggccatgaa
      961 gcgcaccaag taagatggcg ctggggatga agaaccaggc gtttccttgc tgaagaagag
     1021 ttgtaaattc ggtcatgata ataattctta gtattaattc ggcaatctga ttctactccc
     1081 ccccagtacc tgatactacc ccccagtaga ttaatagtgc tatgattttt catattcttg
     1141 ttaaccaggt gttgccatgt ctcatacaat ccgtgataaa cagaaactga aagcgcgtgc
     1201 cagtaagatt cagggccagg tcgtggcgct caagaaaatg ctcgacgagc cgcacgaatg
     1261 cgctgcagtt ttacaacaga ttgctgctat ccgtggcgcg gtaaacggtc tgatgcggga
     1321 agtgattaaa ggtcatctga cggaacacat cgttcaccag ggggatgagc taaaacgtga
     1381 agaagatctg gatgtcgttc tgaaggtgct ggattcatat atcaaataat ttattaacgc
     1441 gattgtaaaa ctgccgtttt tcctcgttta caacgcgtgc gctggacatt accatcctcc
     1501 tctgcgattt atcatcgcaa ccaaacgact cggggtgccc ttctgcgtga aggctgagaa
     1561 atacccgtat tacctgatct ggataatgcc agcgtaggga agtcacggac caccaggtca
     1621 ttgcttcttc acgttatggc aggagcaaac tatgcaagtc gacctgctga gttccgcgca
     1681 atctgcgcac acgttacacc tttttcacca acattcccct cttgtgcact gcatgaccaa
     1741 cgatgtggtg caaaccttta ctgccaatac cttgctggcg ctcggtgcat cgccagcgat
     1801 ggttatcgaa accgaagagg ccagtcagtt tgcggctatt gctagtgcgt tgctgattaa
     1861 cgttggcacg ctgacgcagc cacgcgcaca ggcgatgcgt gccgccgttg agcaagcaaa
     1921 aagctctcaa accccctgga cgcttgatcc agtagcggtg ggtgcactcg attatcgccg
     1981 ccgtttttgt ctggaacttc tgtctcataa gccaaccgcc atacgtggta atgcttcgga
     2041 aatcatggca ttagctggcg ttgctaatgg tggacgggga gtggatacca ctgacgccgc
     2101 agctaacgca atacccgctg cacaaacact ggcacgggaa actggcgcaa tcgtcgtggt
     2161 cacaggcgag gtggattatg ttaccgatgg gcatcgtatc gttggcattc acggtggcga
     2221 tccgctaatg accaaagtgg taggaactgg ctgtgcatta tcggcggttg ttgctgcctg
     2281 ttgtgcgtta ccaggcgata tgctggaaaa tgtcgcatct gcctgtcact ggatgaaaca
     2341 agccggagag cgcgcagtcg ccagaagcga ggggccaggc agttttgttc cacatttcct
     2401 tgatgcgctc tggcaattga cgcaggaggt gcaggcatga aacgaattaa cgctctgacg
     2461 attgccggta ctgatcccag tggtggtgcg gggattcagg ccgatcttaa aaccttctcg
     2521 gcacttggcg cttatggttg ctcagttatt actgcactgg tggcgcaaaa tacccgtggc
     2581 gtacagtcgg tgtatcgcat tgagcctgat tttgtcgccg cccagctcga ttcggtgttc
     2641 agcgatgtgc gaatcgacac cactaaaatc ggtatgttgg cggaaaccga tattgttgaa
     2701 gcggtggcag aacggttgca acgttatcag atccaaaacg tggtactcga caccgttatg
     2761 ctggcaaaaa gcggcgaccc gctgctttca ccttcggcgg ttgctacgct gcgcagtcga
     2821 ttattgccac aggtttcatt aataacgcca aacttgcccg aagctgccgc cttgctcgac
     2881 gcgccacacg cgcgcaccga acaggaaatg ctggaacaag ggcgatcgct gttggcgatg
     2941 ggctgtggcg cagtgctaat gaaaggtggt catctggatg atgagcaaag cccggactgg
     3001 ctgtttaccc gcgagggtga acaacggttt accgcaccgc gcattatgac caaaaacacc
     3061 cacggtactg gttgtacact ctctgcggcg ttggctgcac tacgcccgcg ccatacaaac
     3121 tgggctgaca ccgtacagga ggcaaaaagc tggctttcat cggcgttagc ccaggccgac
     3181 acgctggaag ttggtcacgg tattggtccg gttcaccact tccacgcctg gtggtgagca
     3241 ccgtactata gctgctttgg ttattgtcag cctttggcac tgacattcag ggatgaaacc
     3301 aatgcagcta agaattacca gtcgaaaaaa actcaccgct ctattatgcg ctcttgggct
     3361 aatttccatc gttgcaattt atccgcgtca gacggtgaat tttttctatt cgacagcagt
     3421 tcagattaca gactacatcc acttctacgg ttatcgcccg gttaaatctt tcgctattcg
     3481 tattcctgcc agttacacca ttcacggaat agatgtttcg cgctggcagg agcggatcga
     3541 ctggcagcgt gtggcaaaaa tgcgcgataa cggtattcgc ttacaatttg cctttattaa
     3601 ggcgacggaa ggcgaaaagc tggtggaccc ctatttttcg cgtaactggc aactaagccg
     3661 tgaaaatggc ctgctgcgcg gggcatatca ttatttctcc ccgtcggtag cagcaccggt
     3721 tcaggtgcga ttatttctgc aaacggtaga tttctcacaa ggcgatttcc ctgcggtact
     3781 ggacgtagaa gagcgaggaa aattatcggc aaaagaatta cgcaagaggg taagtcagtg
     3841 gctaaaaatg gtcgaaaaaa gtacggggaa aaagccgatt atttactcag gagccgtttt
     3901 ttatcacacg aatctggcgg gctatttcaa tgaatatccg tggtgggtgg ctcactacta
     3961 tcaacgtcgt ccggacaatg acggcatggc ctggcgcttc tggcagcatt ccgaccgtgg
     4021 acaggtagat ggcatcaatg gtccggtgga ttttaatgtg tttaatggca cggtggagga
     4081 gttgcaggga ttcgttgatg ggattaaaga aacgccttaa ataagaagca gtgatatact
     4141 cccaccagac tccctggcag gaaaaagaca aatggaacaa gcgcataccc agctgattgc
     4201 ccaactgaac gaacgtattt tagcggcgga taacacgccg ctttatatta agtttgccga
     4261 aacggtaaaa aatgccgtgc gcagcggggt gctggagcat ggcaatattt tgcccggtga
     4321 gcgtgattta agtcagttaa ccggcgtttc gcgcattacg gtgcgcaagg cgatgcaggc
     4381 gctggaagaa gagggtgtgg tgacgcgttc gcgcggttac ggcacgcaga tcaacaacat
     4441 cttcgaatac tcgttgaaag aagcgcgagg tttttctcag caggtggtat tgcgcgggaa
     4501 aaagcccgat acgctatggg ttaacaagcg tgtcgtgaaa tgtcccgaag aagtcgcgca
     4561 gcagctggcg gtcgaagcag gaagtgatgt ctttttgctt aagcgtattc gctatgtcga
     4621 tgaagaagcg gtatcgattg aggaatcgtg ggttccggcg catttaattc atgatgttga
     4681 tgccatcggg atttcacttt atgactattt ccgcagccaa cacatttacc cacaacgtac
     4741 gcgttcccgc gttagcgccc ggatgccgga tgccgagttt cagtcacata ttcagttaga
     4801 tagcaaaata ccggtgctgg tgatcaagca agtggcgctt gaccaacagc aacggcctat
     4861 tgagtacagc attagccact gtcgcagcga tctatacgtt tttgtgtgcg aggagtagtt
     4921 cctcgcgcgt tggcgcacaa tcaccgcccc gatgcccgac aacccacgac gccactgcat
     4981 tgcccagtaa tacggcatcc gccagtggca gaccagaggc cagcccggca agtacgccac
     5041 cggcatgact gtcgcccgcc ccaatggtgt cgacaacttg cgttggaaat gccggaatgc
     5101 agccagaagc gtcgttgctg aaataccatg cgccttcttt atcgagacga acgatcaacg
     5161 gcgcggcaaa tttctcctgc cattgcttgc caagtgttgt tatctctgcg gataaagcaa
     5221 aacgttcggc ggcaatctca gcctcttgac gattgagtga cactaaaggt cgacaggcca
     5281 tggtccgcgc cagtaatgcg tccgggatat cgccaatacg tgggccaaaa tcgataaacg
     5341 gcgtcacgtc ttgcagctct tccagccatt ccactgattt tccacaccgc tgaatgacat
     5401 aaaagtacgt tcgccatccg gctcaaccag cgccagacac cagccgttat cgccttcggc
     5461 gttatcgatc aggctgatta acccctcttt cgccatccgg ttgcgaataa tctccgccca
     5521 taccccctga ccgagcggca aggcattacc cgcttcgatg ccgaggcgct ttaacgccac
     5581 ggcaatattc agtgcgcagc cgccaacgtt aacgctctgc tgtttcagtt cgatatcgca
     5641 cccacgccag gggaggacat aagcgtcggc gatcacgtca atgaccgccg cgccgacgac
     5701 catcaccgac tgacgcgtgg ttaattcagg cagcagcgtg tgtaatcgag cgccgctcat
     5761 accgcctccc gttgttgacg atatttcgcc agcgctgtgg catagcggtt gaaatcaagc
     5821 tgatttaccg catccagctc cgcctttaac ggaggatcga tagcgttaat gccatgcaac
     5881 gcgccgcaga ttgccgtcgc catagcaccg atggtgtctg tatcgccgcc aaggttagcg
     5941 cagaggacgg cgcaacggtt cgggtcggtt tgtgccagtt caaccagcgc aatggcgcac
     6001 ggaacggact cgatagtgct ggtacctgcg ccaacgacct gatacagctg ttcgctggcg
     6061 gattcagtgc cgtcggcatt gcgcacaatt ttcagcgcaa tctccagacg tgctgccagt
     6121 gaggcgctga aggtggtgat gcgtttttgc tgggcatgtc gcgcaattga aggcagtgaa
     6181 tctacaatcg ctgaccagct ttctccgtca atggcacgag aaatcgccca tgcgatgact
     6241 accgcgcccg caaccgccag atcggattta tgggtcgggc tggacgccag cgccacatca
     6301 tcaataaagg aattaacatc acgcgccgga agcaaacaac ctaatggcga gacgcgcatt
     6361 gccgcgccgt tggtcacgcc attgttttcc agttcagcaa cgggtttacc gtcgcgaatg
     6421 gcgttgagag caatcttcga ggtcggacct agtacgtttt tgttaaaggc gtcgaaacgc
     6481 agcgcccagt cgagaatatt acgcccaata agattcggat cgatcttgcc ttcacgttcc
     6541 agtaacgcat ccgccagaca cagcgccatc gaggtatcgt cggtgaattc ggcgcggtta
     6601 aaataacagg ccgcgttatt ctcctttggt ccaggaagaa aacggtcaat ccagccaaag
     6661 tgtgctttaa cgcggctgcg tggccaaagc tcggagggca tccccatcgc atcccctaac
     6721 gcctgcccat aaagagcacc gagaatacgt tctgttttca tttaacttcc ccttgtgtca
     6781 acgcaatatc gcgatcatcg accttgatag ccgtaatttc gttgtcggat tcgcggaaaa
     6841 aaatcatgaa cagcacggcg ataatggcaa tcatcaccgc gccgaaagtc cacatcccgg
     6901 accagttgaa agtcagtccg tttaccggtt cctgataagc gaacatcttt tccatcatca
     6961 caccgccaag acgatagccg agcaaactgc cgaagccctg gcagcagagc gtgatcagcc
     7021 cctgcgcagc ggtacgcata tgcacggggg cttttttatc gacatagatg taagcggtaa
     7081 cgtagtaaaa atcgtaactt acgccgtgaa gcaaaatacc gaggaacagt aacgcgtagg
     7141 tgaaatattc atccgcacta ccgtaaataa agaagccata gcggatcgca gcggtgacca
     7201 gaccaagcaa taataccttt ttgataccaa agcgtttagt gaaaaacggc aatgccagca
     7261 taaagaagat ttcagagaac tggccgagcg tcatccagcc ggtggcgttt ttcatgccaa
     7321 cttcggtcag ataaccgttg gcaaagatgt aatagaacgc cagtggcatc gcaaacagga
     7381 atgaacagaa gaaaaagacg aggaagtttt tatcgcgcag caggatcagc gcatccaggc
     7441 cgagcatgac tttaatatcc attttgccgg tgctttttgg tggcgtgtcg ggcaggaaaa
     7501 acgcaaacac accgagcaga gcagaacttc cggcggtaat cagcagcggg atgttagtcg
     7561 gtgagatatc ggcatacccc agtatttgcg gcaagaaacc acatgccaga ccggaggcaa
     7621 tccagccgat agtgcccatc acacgaatgc gcgggaaatc acgctcaaca tccggcacgt
     7681 tggcaaaagc gatgctgtta gtcagcgcaa tggtcggcat ataggttagc gagtaggcca
     7741 gcagtaacgg gaagaacccg gcaaaagtgg tctgttgcgc agcgaaatac atcagcagcg
     7801 cgcctgcgaa catcaatacc gccagcactt tctgcgccga gaaaaagcgg tcagtgatgg
     7861 agccaaccag aattggcgac aggatcgccg caatggcggt acaggcatac gaccagccaa
     7921 tttctccggc actaaaaccg cttttactta accacaacca caatggcaca aaccacgcgc
     7981 cccagataaa ccattcaaca aacatcatga acgacagctt tgctgttgtt ttcatctttt
     8041 aattccttca tgacaggtaa tgtaaggtac gcgatgacaa taccacttaa taataccttt
     8101 taaatacctt tgcagtgaaa acttgaccgc gctaacattt tttctgatga atcgagccaa
     8161 cagaaaacgc tgaaaaaaca tccaaaagat ggaaaaactc gcccgtaacg accaggatta
     8221 tgattgcccg caaaaggcgg gccaggaaaa tccatagccg atatccaatc ggaatttacg
     8281 ggagcatagt aatgacagat attgcgcagt tgcttggcaa agacgccgac aaccttttac
     8341 agcaccgttg tatgactatt ccttctgacc agctttatct tcccggacat gactacgtag
     8401 accgcgtgat gattgacaat aatcgcccgc ctgcggtgtt acgtaatatg cagacgttgt
     8461 acaacaccgg gcgtctggct ggcacaggat atctttctat tctgccggtt gaccagggcg
     8521 ttgagcactc tgccggagct tcatttgctg ctaacccgct ctactttgac ccgaaaaaca
     8581 ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc gtcaacttac ggcgtgctgg
     8641 cgtcggtatc gcggcgttat gcgtatcgca ttccattcct cgtcaaactt aatcacaacg
     8701 agacgctaag ttacccgaat acctacgatc aaacgctgta tgccagcgtg gagcaggcgt
     8761 tcaacatggg cgcggttgcg gttggtgcga ctatctattt tggctcggaa gagtcacgtc
     8821 gccagattga agaaatttct gcggcttttg aacgtgcgca cgagctgggc atggtgacag
     8881 tgctgtgggc ctatttgcgt aactccgcct ttaagaaaga tggcgttgat taccatgttt
     8941 ccgccgacct gactggtcag gcgaaccatc tggcggcgac cattggcgcg gacatcgtca
     9001 aacagaaaat ggcggaaaat aacggcggtt ataaagcgat taattacggt tataccgacg
     9061 atcgcgtgta cagcaagtta accagcgaaa acccgattga tctggtgcgt tatcagttag
     9121 ctaactgcta tatgggccgg gcagggttga taaactccgg cggtgctgcg ggcggtgaaa
     9181 ctgacctcag tgatgcagtg cgtactgcgg ttatcaacaa acgcgctggc ggaatggggc
     9241 tgattcttgg acgtaaggcg ttcaagaaat cgatggctga cggcgtgaaa ctgattaacg
     9301 ccgtgcagga cgtttatctc gatagcaaaa ttactatcgc ctgatacgcc ttatacgaac
     9361 aacaccaccc cgtaggacgg ataaggcgtt tacgctgcat ccggcaacct atgcctgatg
     9421 cgacgctgaa gcgtcttatc atgcctacat agcactgcca cgtatgttta caccgcatcc
     9481 ggcataaaaa cacgcgcact ttgctacggc ttccctatcg ggaggccgtt tttttgcctt
     9541 tcactcctcg aacaattttc atattgtcgt ttttgtgatc gttatctcga tatttaaaaa
     9601 caaataattt cattatattt tgaaatcgaa aacaaacagc aggatatgaa aatgtacgtg
     9661 gtatcgacaa agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc
     9721 aatattcaca atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg
     9781 ccggtcatca tcgcaggaac gcctggcaca tttactcatg ctggtacaga aaatctgttg
     9841 gcgctggtca gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat
     9901 cacacgaaat ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt
     9961 gacgcctcgc atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt
    10021 tgccatcgct ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat
    10081 gatgtgcaaa tcaatgaagc cgatgctttt tataccaacc ccgctcaggc gcgtgaattt
    10141 gccgaggcaa ccggaattga ttccctggcg gtcgccatcg gcacggctca tggtatgtat
    10201 gccagcgcac cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta
    10261 ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg atattcagca aaccatcaaa
    10321 ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta
    10381 aaaaattacc tgaccgagca ccccgaagcg accgatcccc gggattattt gcagtcggct
    10441 aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg
    10501 gcataacgca cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa
    10561 agctggtgaa catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc
    10621 ggcgctggca tttcatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca
    10681 ggtcaatcaa tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt
    10741 tacgattgcc gataaagttg ggtttgcacg cgaacgcatt attctcggcg gcgatcatct
    10801 ggggccaaac tgctgacagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct
    10861 ggtaaaagca tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg
    10921 cgcgggggat cccataccgt tagcaccaga aacggttgcg gaacgagctg ctgtgctttg
    10981 ctttgctgcg gaaagtgtgg cgacagattg ccagcgtgag caactgagct atgtcattgg
    11041 caccgaagtt ccggttccgg gcggtgaggc cagcgccatt cagtcagtac acatcaccca
    11101 tgttgaagat gccgccaata ctttacgtac gcatcaaaag gcctttattg cccgtgggct
    11161 gacagaggcg ttaacacgtg tgattgccat cgtggtgcag ccgggtgtgg aatttgatca
    11221 cagcaatatt atccattatc agccgcagga agcgcaggcg ctggcgcaat ggatagaaaa
    11281 cacccgaatg gtttatgaag cacattctac cgattaccag acccggacgg cttattggga
    11341 attagtccgc gatcactttg caatattgaa agtcggtccc gcattaacct ttgctttacg
    11401 cgaggcgata tttgcactgg cacaaattga gcaggaactt atcgcccccg aaaatcgcag
    11461 cggttgcctg gcggtaattg aagaagtgat gctcgacgaa ccgcaatact ggaaaaaata
    11521 ttatcgtacg ggttttaacg attcattact ggatattcgt tacagcctgt cggatcgtat
    11581 tcgttattac tggccgcata gtcggattaa aaatagcgtc gaaacgatga tggtgaatct
    11641 tgaaggcgtg gacatcccac tgggcatgat tagtcagtat cttcccaaac aatttgaacg
    11701 cattcagtcc ggggaattat cagcaatacc gcatcagctg attatggata aaatttatga
    11761 tgttttgcgc gcctatcgct acggctgtgc ggaataagga cggtatatga ctaacctgtt
    11821 tgttcgtagc ggaatttatt ttgtcgatcg tagcgaagtt ttaacccata tcggtaatga
    11881 gatgctcgcc aaaggtgtgg ttcatgatac ctggccacag gcattaattg ccagagaagc
    11941 agaattccct accgggataa tgcttgagca gcacgccatt gcaataccgc attgtgaggc
    12001 gattcatgct aagtcgtcag ccatttatct gttaaggcca acaaataaag ttcattttca
    12061 gcaagcggat gatgataacg acgtggaggt atcgttggtt attgcgttga ttgtggaaaa
    12121 tccgcagcag caattgaaac ttttacgctg tttatttggc aagttacaac agcccgatat
    12181 cgtcgagaca ctaatcactc ttcctgaaac ccagttaaag gaatacttca caaagtatgt
    12241 tttagattca gacgaataaa tccctctgta acaataaaaa ggactattta tgaaacgcaa
    12301 gattattgtc gcttgcggag gcgcggttgc gacctctacg atggcggcgg aagaaattaa
    12361 agagttgtgt cagaatcata atattcctgt tgaattaatc cagtgtcggg
//
LOCUS       DAJTNA010000120        12322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTNA010000120 DAJTNA010000000
VERSION     DAJTNA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.121"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..611
                     /locus_tag="OO161_002805"
     CDS             <1..611
                     /locus_tag="OO161_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5463808.1"
                     /translation="ILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQMIAL
                     RIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDYSWI
                     VQPVANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTELGSRIICRDKQLIFE
                     EAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKSSGGKKG"
     gene            608..1222
                     /locus_tag="OO161_002806"
     CDS             608..1222
                     /locus_tag="OO161_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR5463809.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OO161_002807"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OO161_002807"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5463810.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEISAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="OO161_002808"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="OO161_002808"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5463811.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="OO161_002809"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="OO161_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5463812.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="OO161_002810"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="OO161_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5463813.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPALEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="OO161_002811"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="OO161_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5463814.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTGNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="OO161_002812"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="OO161_002812"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5463815.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPGG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="OO161_002813"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="OO161_002813"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5463816.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNVSTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="OO161_002814"
     tRNA            9115..9190
                     /locus_tag="OO161_002814"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9356..10570
                     /locus_tag="OO161_002815"
     CDS             9356..10570
                     /locus_tag="OO161_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5463817.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPNGGKYWRLKYRV
                     AGKEKLLALGVYPEVTLADARAKREDAKRGIAGGIDPMEAKREEKIARETQLNNTFKD
                     IALEWHSSKLKKWSAGYASDILEAFNKDVFPYIGKKPIAEIKPLELLNVLRRIEGRGA
                     TEKAKKVRQRCGEVFRYAIVTGRAEYNPAPDLTSAMQGHESNHYPFLTAKELPDFFKA
                     LSSYSGSALVVMAARLLIIIGLRTGELRGALWDEIDFNKAIWEIPASRMKMRRPHIVP
                     LSDQALSLIGKIREITGNYPLMFPGRNDPRKTMSEASINQVFKRIGYAGRVTGHGFRH
                     TMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDALEKE
                     ENVVHGSFGKRA"
     gene            10713..11594
                     /locus_tag="OO161_002816"
     CDS             10713..11594
                     /locus_tag="OO161_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463818.1"
                     /translation="MVRAKKQDLAWFDISNYEFLNDLTLPDLIQELEWRDFLLRHAKD
                     DTAIFKEEYDIKYERIFSGDPNLTILNEEEEEVEEFIRKVNDEAPSLRNEYDDLPSLS
                     SAMGVSPVTFSELAMYSFSSIDQGFFKRDEEDCYLKANAMLASVTGNLSNCSPNIILV
                     SIDLDDATDDEIITSLTHLLPLWRKELKVPEREHVAQKRIGLKTLQKLISNRVIPIVD
                     LLIWGEKSGKEVSNPMISALVFSDDPKDTQAIKESIKPFALESISERYTRLLQLYVNK
                     DREMSLIKISDLMSRDL"
     gene            11792..11989
                     /locus_tag="OO161_002817"
     CDS             11792..11989
                     /locus_tag="OO161_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015462155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5463819.1"
                     /translation="MSQSFIRLSEVQRRTGYSKVWIYRLIKEQRFPQSVKIGSRSIAF
                     IESEIEEWINQRIAESRGEVA"
     gene            11989..>12322
                     /locus_tag="OO161_002818"
     CDS             11989..>12322
                     /locus_tag="OO161_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463820.1"
                     /translation="MNKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYATELERQLGITLERIDEKNPDGIGTHLRYRFSCR
                     GDVLKVITH"
BASE COUNT         3166 a         2901 c         3166 g         3089 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgttct cgcatcatgg tttgcctcaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgtgt attaatcgcc
      121 agatgatcgc tttgcgcata atgcaacaag ttaaggccac gggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcacgt ggtgattact
      301 cctggattgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggcag
      361 ggcgtaaatg ggggcgcacc gagtgtaaat ggcgtctggc agcgaaatac acggtgacgc
      421 aactctcgcg gactgaactt ggcagtcgga taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggccg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaagc agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggaca ggctaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgt ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggagc aatccgatgc gatccggtat gagacgctgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccga aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgtcttgttg aacaggcgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagtg aacggatcag gcagtgaatt tctacgttat tgtcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc aaccacacct ttggctacat cggagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca gtgcgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcaggtc cagttcttct gcatgacccg ccaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tcaccgccgg agtgaccgcc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccaaa
     2281 agcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg gaccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagtgc agaggccata ccaatgccgt tatccgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccggt ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgagagctta aagtgctgaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat agttattgat gacctggtgg ataccggtgg tactgctgtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat attccgcaag atacctggat cgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcaggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgttt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggct
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggtattg acccacgtga aatcctcgac gtccaggcac gtattgtgat gagcgatgct
     3781 gaacgtaccg acgatgattt atacgatact gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccacccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgacactg gctgcatgcg gctacgttgt acaacattgc cgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa
     4021 gaggccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgtgat gctgactatt gatatgccgt cggtgggctt ttcttcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg tcttaaaggc gctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgct gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctgggac gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaattttgcc aatttggtaa aacagttgca tcacaacagg agatagcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac tggaagtgcg tgaattctgg ggctggtgga tggagctgga agcgcaggaa
     5041 tcccgtttta cctacagcta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagatactga agtggttgaa cgactggagc acaccctgcg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgacgagc cggtgaagtt aacggcgtga gtgaaatgtg ccggatgcat cacatccggc
     5281 aatatctatt aaaactgata cgtcatccca atcgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaatgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattaacca gatcttcacc accgatgccc
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gagtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgccggtctg cgccgaggag
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttttgct gtgcagtatc actctctgct ttattaccgg caaactctgc ttcccaacga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcgcctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact gtcgttatca ctcatataat gcatggcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttgtta tatatttctg cggcctgtac ggatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa aaaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atatcgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcactt gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggggggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg tcgcgatatc gcccacggtg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgcc gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacctgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgtc tctacgcgtt tcaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgagcgtaa
     9001 ataaaaccgg gtgatgtaaa agtagccatt tgattcacaa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttcaaacatc tccccaagtc tactaaagtc tttcaaaacc ccttataatc
     9241 cgcgtattaa agccccgttc gtcttttgac gcctactaaa gtccctcaaa atctacggtc
     9301 agatgggggt acttatgggg gtatttgctg ttcggtttag tggaggtacc cccaagtgaa
     9361 actcaatgcc cgtcaaatag acacagccaa gccaaaagag aaggcttaca agctggccga
     9421 tggtggcggt ttgtatctct tggtaaaacc taatggaggt aaatactggc gacttaaata
     9481 tcgtgtagct ggtaaagaga agctattggc actaggtgtg tatcctgagg ttactctagc
     9541 cgatgctcgg gcaaaacgtg aagatgcgaa aagaggtatc gctggtggta tcgatccgat
     9601 ggaagcgaaa cgagaggaaa agattgcccg ggaaacgcag ttaaacaaca ccttcaaaga
     9661 tattgccctt gagtggcaca gcagcaaatt aaaaaaatgg tctgctggtt atgcttcaga
     9721 catcctcgaa gccttcaaca aagatgtgtt cccttacatt ggcaaaaaac caatcgccga
     9781 aatcaaacca cttgaacttc tgaatgtgct gcggcgcatc gaggggcgcg gtgctacaga
     9841 aaaagccaaa aaagtgaggc agcggtgcgg ggaagttttc cgctatgcaa tagtcactgg
     9901 tcgcgcagag tataaccctg caccagacct tactagcgcg atgcaaggcc atgaatctaa
     9961 tcattaccct ttccttacag ctaaagaatt acctgatttt ttcaaggcat tgtccagtta
    10021 ctcaggaagc gcattggttg ttatggcggc tcgtctactg attatcatcg gcttgcggac
    10081 tggcgaacta cgtggcgcat tatgggatga aattgatttc aacaaggcta tctgggagat
    10141 acccgcttca cgtatgaaaa tgcggcggcc tcatattgtg ccattgtctg atcaggctct
    10201 ttcgcttatt gggaagatta gagaaataac aggcaattac cctcttatgt ttcccgggcg
    10261 caatgatcca aggaaaacaa tgagtgaggc cagcataaac caagtgttta agcgcattgg
    10321 ctacgctgga cgtgtaactg gtcatgggtt ccggcacact atgagcacga ttttgcatga
    10381 gcagggctat aacaccgcgt ggatagaaac gcagctcgct cacgttgata agaactcaat
    10441 tcgtggcaca tacaaccatg cgcaatatct ggatggaagg cgggagatgc ttcaatggta
    10501 tgccgactat atggatgcgc tggagaagga ggagaacgtt gttcatggtt cgtttgggaa
    10561 acgagcttaa ctgtatgaat agaccgtgtt aagcaatggt agtagactta tgtagacgaa
    10621 caaagaatag gctatgtcta ggctgatccc cgaaaacccg tacaccccta cgggctggca
    10681 tagccgtcag aattaggggc gcggggtggc gtatggtgag agccaaaaaa caagatttag
    10741 catggtttga tattagtaat tatgaatttt taaatgattt aaccttgccg gacttgattc
    10801 aagaattaga gtggcgcgat tttttattgc gtcatgcaaa agatgacact gctatattca
    10861 aagaagagta cgatataaaa tatgaaagga ttttttcggg cgatcctaac ctaaccattc
    10921 tcaatgaaga agaggaagag gtcgaagaat ttattcgcaa agtaaatgat gaagccccat
    10981 cattaagaaa tgagtacgat gatctaccct cattatcttc agctatgggg gttagtccag
    11041 taacattttc agagctggcc atgtactctt tctcatcgat agatcaaggc ttttttaaaa
    11101 gagacgaaga ggattgctac ctcaaggcaa acgctatgct tgcgagtgtt actggaaacc
    11161 taagtaattg ttcccctaac atcatactcg tttctataga tttagatgac gccactgatg
    11221 acgaaattat taccagcctg actcaccttc tacctctatg gcgaaaagag ttaaaagtac
    11281 cagagagaga gcatgtcgct cagaaaagga ttggattaaa gacccttcag aagttgatat
    11341 ctaatagagt gatacctatt gttgatctgc tgatttgggg tgagaagtca ggtaaggaag
    11401 tgagcaatcc gatgatttct gccttggttt ttagtgatga tccgaaagat acgcaggcta
    11461 ttaaggaatc aataaagcct tttgcacttg aatctataag cgagagatac acccgcttgc
    11521 ttcagcttta tgtaaacaaa gacagagaaa tgagcttgat aaaaatatct gatttaatga
    11581 gtagagattt atagcaagca aaatcctacg taaacgtttt aagtaaattt cgcgatttcc
    11641 gctttgaata cgctactgtg tatttcaaag cggataatct tctcaaaaaa atattttatt
    11701 tttatccgct atcacccatt caccggtttg tttaatctcc tttctgtcta ctgatgtcca
    11761 ttaaagaaga tgacagaata taggagtaaa catgtctcaa tcatttattc gtctttctga
    11821 ggttcaacgc cgtactggtt acagcaaagt gtggatttat cggctcatca aagaacagcg
    11881 attccctcaa tctgtaaaga ttggaagccg atcaattgcg tttattgaaa gtgaaattga
    11941 agaatggatt aatcagcgca tcgctgagtc gcgtggcgag gtagcgtaat gaataagaaa
    12001 aaccgcccat tacaggcggc taattcagat attcgcgtat ctgatgttac gccccttaca
    12061 aaatcccttc aagcaccaaa gcgcacaccg aaaaagcatc gtgccagagt ctatatgctg
    12121 cgtactggta tagagggatg gacagaaaat gacattcttc gctactgccg tctgtcttct
    12181 ggtcgtaact atgcaacaga gttagaacgc cagcttggaa tcactctgga acgtatcgac
    12241 gaaaagaatc ctgatggtat cggaacacac cttcgctacc gtttctcctg ccgtggtgat
    12301 gttctgaaag tgatcactca ta
//
LOCUS       DAJTNA010000121        12111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTNA010000121 DAJTNA010000000
VERSION     DAJTNA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12111
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.122"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..267)
                     /locus_tag="OO161_002819"
                     /pseudo
     CDS             complement(1..267)
                     /locus_tag="OO161_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; frameshifted;
                     incomplete; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            456..986
                     /gene="cybB"
                     /locus_tag="OO161_002820"
     CDS             456..986
                     /gene="cybB"
                     /locus_tag="OO161_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5463821.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1231..1404
                     /locus_tag="OO161_002821"
     CDS             1231..1404
                     /locus_tag="OO161_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463822.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(1476..1625)
                     /gene="hokB"
                     /locus_tag="OO161_002822"
     CDS             complement(1476..1625)
                     /gene="hokB"
                     /locus_tag="OO161_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5463823.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2024..3664
                     /gene="trg"
                     /locus_tag="OO161_002823"
     CDS             2024..3664
                     /gene="trg"
                     /locus_tag="OO161_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5463824.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDESAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVSSVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(3702..4631)
                     /locus_tag="OO161_002824"
     CDS             complement(3702..4631)
                     /locus_tag="OO161_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5463825.1"
                     /translation="MAYGKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPAL
                     SKTLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNN
                     DVVRIGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRM
                     SDPELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSD
                     ALVQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGH
                     GAGEPIGILTRVDAMFSSGCQLMINAIRKSMPF"
     gene            4841..6184
                     /locus_tag="OO161_002825"
     CDS             4841..6184
                     /locus_tag="OO161_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5463826.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILQRQTSFRALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            6409..8064
                     /gene="opgD"
                     /locus_tag="OO161_002826"
                     /gene_synonym="mdoD"
     CDS             6409..8064
                     /gene="opgD"
                     /locus_tag="OO161_002826"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5463827.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8204..8428
                     /locus_tag="OO161_002827"
     CDS             8204..8428
                     /locus_tag="OO161_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5463828.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            8491..9027
                     /gene="rimL"
                     /locus_tag="OO161_002828"
     CDS             8491..9027
                     /gene="rimL"
                     /locus_tag="OO161_002828"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5463829.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQIALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9022..10001)
                     /locus_tag="OO161_002829"
                     /pseudo
     CDS             complement(9022..10001)
                     /locus_tag="OO161_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10125..11117
                     /gene="tehA"
                     /locus_tag="OO161_002830"
     CDS             10125..11117
                     /gene="tehA"
                     /locus_tag="OO161_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5463830.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11114..11707
                     /gene="tehB"
                     /locus_tag="OO161_002831"
     CDS             11114..11707
                     /gene="tehB"
                     /locus_tag="OO161_002831"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5463831.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            12010..>12111
                     /locus_tag="OO161_002832"
     CDS             12010..>12111
                     /locus_tag="OO161_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5463832.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGF"
BASE COUNT         3112 a         2804 c         3028 g         3167 t
ORIGIN      
        1 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
       61 ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaatgg tccgtagttt
      121 gaatcatgtt tcagcaggta ggcgagaatt tttggggaag tgagatcatt aatagcgaca
      181 acgtctatgt ttgcttttga cttcaagtaa tcgacgcaac accagtcgac cgatacgacc
      241 aaaaccgtta ataccaacct tactcatggt tttctcctgt caggaacgtt cggatgaaaa
      301 ttgatccttt ccaggcttag cccaggattg tgggatgggc aatctccatt cttacagtga
      361 aacgtaacgt actgaaaacg ggtgaacaat atttaatgaa attttgagaa aagcccgtta
      421 tgttaacgga acattatgtt aaagcaggaa atgttatgga aaataaatac tcaaggttac
      481 aaatcagcat tcactggctg gtctttttgc tggttatcgc agcgtattgc gcaatggagt
      541 ttcggggttt cttcccacgt agcgatcggc cactcatcaa catgattcat gtttcctgcg
      601 gcatctcaat tctcgtgctg atggtcgttc gtcttttgtt aaggctgaaa tacccaaccc
      661 cgccgattat acctaagcca aaaccgatga tgacgggact ggcgcatttg ggacatttgg
      721 tgatttatct cctgtttatt gcgctgccag tgattggttt ggtgatgatg tataaccggg
      781 gcaacccgtg gtttgcgttt ggtttgacga tgccttacgc ttcagaggcc aatttcgaac
      841 gggttgatag cttaaagtcg tggcatgaaa cgctggcgaa tctgggatat tttgtcatcg
      901 ggttgcacgc tgcggctgca ctggcacacc actatttctg gaaggacaac acacttctac
      961 gcatgatgcc gcgtaaacgt tcctgaagga tatttaaaga aaacgcctgt actaaaaccg
     1021 acccgtggta caggcgaaga atacgggtct acatcggaag cgcctattat atttatttgt
     1081 atgacaaata aaaccataat ccttgcccat acgtccatct ggcttatttt taatcaattc
     1141 acccgatctt tgatctcatc aacggtatca aaataaacca gcgtataacg ttcaaatcga
     1201 acataagatt cgataaacca tggaggttat atgaaaaaac tggcacttat tttgtttatg
     1261 ggaacgcttg tttcctttta tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg
     1321 gggatctcac actgtacggc aggcggcaaa tttgtctgta atgatggttc tattagtgcc
     1381 tcgaaaaaaa catgcactaa ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct
     1441 agtcagatgc gcggggaaaa tccccgcgct tgcccttacc tggacgtgca ggccatgagc
     1501 gcagcaacct ccttatcacc gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg
     1561 agtgtgaatg tcagaatcgt aatgcagata atgagcagac acaccaccag agggttgtgc
     1621 ttcatagcct ttctccttgc cggatggcgg gtaagaggct aagatctgaa ttgctaggtt
     1681 cattcgttgg cctcggttga tagaaatatc ggtcggggcc ttcgtctttc tgattcccgg
     1741 ttagcctgaa aacagaaagt ctcaggcacc cgcaggcatc ctatgaggtt tccttaggga
     1801 cgaaaataat cacttcacga aattgcgtgc tgttttccag aatttttcgt cattcggatt
     1861 agccagttta gccattcgtt actctcttca ttccaatagc attaattttc tctgcaataa
     1921 ttgttgtaaa aatgtgacgc aaagaggttt ttggtcataa gtaattaccg tcaagtgccg
     1981 atgactttct atcaggagta aacctggacg agagacaacg gtaatgaata caactccctc
     2041 acagcgatta ggttttttgc atcacatcag gctggttccg ttatttgcct gcattcttgg
     2101 cggtatctta gttctattcg cattaagttc tgctctggct ggctatttcc tctggcaggc
     2161 cgatcgcgat cagcgtgatg ttactgcgga gattgagatt cggaccgggt tagcgaacag
     2221 ttcagatttt ttgcgttcag cccggatcaa tatgattcag gccggggctg cgagtcgtat
     2281 tgcggaaatg gaagcaatga agcgaaatat tgcgcaagcc gaatcggaga ttaaacagtc
     2341 gcagcaaggt tatcgtgctt atcagaatcg accggtgaaa acacctgctg atgaagccct
     2401 cgacactgaa ttaaatcaac gctttcaggc ttatatcacg ggtatgcaac ctatgttgaa
     2461 atatgccaaa aatggcatgt ttgaagcgat tatcaatcat gaaagtgagc agatccgacc
     2521 gctggataat gcttataccg atattttgaa caaagccgtt aagatacgta gcaccagagc
     2581 caaccaactg gcggaactgg cccatcagcg cacccgcctg ggtgggatgt tcatgattgg
     2641 cgcgtttgtg cttgccctgg tcatgacgct gataacattt atggtgctac gtcggatcgt
     2701 cattcgtcca ctgcaacatg ccgcacaacg gattgaaaaa atcgccagtg gcgatctgac
     2761 gatgaatgat gaatcggcgg gtcgtaatga aatcggtcgc ttaagtcgtc atttacagca
     2821 aatgcagcat tcactgggga tgacagtagg gactgttcga cagggcgcgg aagagattta
     2881 tcgtggcacc agcgaaattt cagctggcaa tgcggacctg tcatctcgca ccgaagaaca
     2941 agcggcggct atcgaacaaa ctgccgccag catggagcaa ctcactgcga cggtgaaaca
     3001 gaatgcggat aacgcgcatc atgccagcaa actggcgcaa gaggcttcta ttaaagccag
     3061 cgatggcggg cagacggttt ccggtgtagt aaaaacgatg ggcgctatct ccacgagttc
     3121 gaagaaaatt tctgagatca ccgccgtcat caacagtatt gctttccaga cgaatattct
     3181 ggcactgaat gctgccgttg aagccgcgcg agcgggtgag caagggcgtg gatttgccgt
     3241 tgtcgccagc gaagtacgga cactcgcaag tcgcagcgct caggcggcga aagagattga
     3301 aggcttgatc agtgaatcag tcaggttaat tgacctgggg tcggatgagg tggcaacggc
     3361 cgggaaaacc atgagcacta ttgttgatgc cgtctcgagt gtcacacata tcatgcagga
     3421 aatcgccgcc gcctcggatg aacaaagtag aggcataacg caggttagcc aggcgatttc
     3481 tgaaatggat aaggtgacgc aacagaatgc ttctctggta gaagaggcct cagcggcggc
     3541 ggtgtccctt gaagaacagg cggcacgatt aactgaggcg gtggatgtat tccgtctgaa
     3601 caaacattct gtgtcggcag aacctcgcgg agcgggtgaa ccagttagtt tcgctacggt
     3661 gtgaaaatgt tcaaggaggg atcgacagat cccttcacct ttcagaacgg cattgatttt
     3721 cgaatagcgt taatcatcaa ctggcaacca gaagagaaca tcgcatctac gcgggtcagt
     3781 attccaatcg gttcgcctgc accatgtccc ggaacaggca gggccaccag cgtggcatga
     3841 cgcaggtcgt cttttaccgc gccagaaggg acaaaccaca cgtagtcgta ttcaaccgta
     3901 agttgacgag atagcgaagc agacagcgtt tcgatacaac ccgaaggaat tttacatccc
     3961 tggctctgta ctaatgcatc tgaatgctgg cgtggcgcag tgccttctgg tgatacaacg
     4021 accggccatt ccagcactcg gcttagcgtt acgttctcct gaagtagcgg gtgattaggg
     4081 cggacaacca gcttcagcga ttcaagaaac agcagttcgt aattaagccc ggtcatcagt
     4141 tcaggatctg acatccgacc aatgccgata tcgatttccc cggttttcaa acccgccaga
     4201 atcatagggt tactcattgt cgcaacttgc aaggtcgtct ctttttgttg ctgatgaaac
     4261 tgacctataa ccgaaggtaa tatccccagt gccgcagtag gtagtgcacc aatcctgacg
     4321 acatcattat taagaccttc tttacgatga agcgactgtc cggcagtgtt gatggcgtca
     4381 agaactctga ctgcatgcgt taaaaattgt tcgccgggta aggtaagttg cgccccctga
     4441 cgaccacgct caaacaagcg agcgcccgtc agctgctcca gttcattcaa tgtcttagag
     4501 agcgcaggtt gactcaaatt aagggtttca gccgcgcgcc ccaaagttcc ttgttgtgcg
     4561 acagctacga atgtatgaag gtggcgcaaa cggatgcgct gactaaacag actatttttt
     4621 ccataagcga tgttaaaaac gaagcggtgt cgctgacaag tgaagttgtt tgattatgat
     4681 aacttgattg caaaatataa ttaacaatta aagcaattgt gttacagcaa aatggataat
     4741 attgatgttt tcgcggcgag atcacagttt gtaaattctt cccgccagag tgaatgcggt
     4801 tacctacact ccagattact gaccactgga ggcagacact atggcgaaca gcatcacggc
     4861 ggatgagatt cgggaacagt tttcgcaggc aatgtcagcc atgtaccagc aagaagttcc
     4921 gcagtacggc acgctgctgg aactggtagc tgatgtgaat ctggctgtgc tggaaaacaa
     4981 tcctcaactg cacgaaaaaa tggtaaatgc agacgagctg gcgcgactga atgttgaacg
     5041 tcatggggcg attcgcgttg ggactgcaca agagcttgct actcttcggc ggatgtttgc
     5101 cattatgggg atgtacccgg tgagctatta cgatctctcg caggcagggg tgccggtaca
     5161 ttcgacagca tttcggccca ttgatgatgc ttctctggcg cgtaatccct tccgcgtttt
     5221 tacctcctta ctccgccttg agcttatcga gaacgaaatt ttgcgccaga aagcggcgga
     5281 gattctgcgt cagcgcgata tcttcacccc acgttgtcga caactgttag aggaatatga
     5341 gcagcagggc ggttttaacg aaacacaggc acaggaattt gtgcaagagg ccctggaaac
     5401 gtttcgctgg caccagtcag caacggtaga tgaagaaacc tatcgcgcct tgcacaacga
     5461 acatcggttg attgctgatg tggtctgttt tcctggatgc catatcaacc acctgacgcc
     5521 acgtacgctg gatattgacc gggtgcagtc gatgatgcct gaatgcggaa ttgaacccaa
     5581 aattctcatc gaggggccgc cgcgccgcga ggtaccgatt ttacaacgcc agaccagctt
     5641 tagagcactg gaagagacgg tgttgtttgc ggggcagaaa cagggcacgc ataccgcgcg
     5701 ctttggtgaa attgagcagc gtggcgtggc attaacgccg aaagggcgac aactgtatga
     5761 tgatcttctg cgtaacgctg gaaccgggca ggataatctc acacaccaaa tgcatttaca
     5821 ggaaaccttc cgcacttttc ctgacagtga gtttttaatg cgtcagcaag ggttggcatg
     5881 gttccggtac cgtctgacgc cttcgggtga ggcgcatcgt caggcgattc atcttggaga
     5941 cgatccacag cctttaattg aacgtggttg ggtagtggcg caacccatca cctatgaaga
     6001 tttcttgccc gttagcgcgg cggggatctt ccagtcaaat ctgggtaatg aaacgcagac
     6061 acgcagtcac ggtaatgcca gtcgcgaagc atttgagcag gcgttgggtt gtccggtttt
     6121 ggatgagttc cagctttacc aggaagcgga agaacgcagt aaacgtcgct gtggtttgct
     6181 ttaaattctg accatccgcc tttgcaaaaa tttgcctgat tttacaaacg aatcaggctc
     6241 atcccatcga cataaaaaaa atgccgattt atgcatattc tctcagttca acaattggat
     6301 tattaataaa tattgtctag agtgagcggt cataaataag cactttcttg ccgctgaaaa
     6361 cgaccagcgc gggaccattc acaacaccag aaggactcac tttcaggtat ggatcgtaga
     6421 cgatttatta aaggttcaat ggctatggcc gccgtgtgcg gtaccagcgg cattgcttct
     6481 cttttttctc aggcggcatt cgcggcagat tctgatattg ccgacgggca aacccagcgt
     6541 tttgacttct ccattctaca gtcaatggcg cacgacttag cgcaaacagc gtggcgtggt
     6601 gcgcctcgtc cgttacctga cacgctggcg acaatgacgc cgcaggctta taacagtatt
     6661 caatacgacg ccgaaaaatc gctctggcat aacgttgaga accgtcaact ggacgctcag
     6721 ttcttccata tgggaatggg attccgtcgc cgcgttcgta tgttttctgt agatccagca
     6781 acacatctgg cgcgtgaaat tcactttcgc ccggagttgt tcaaatacaa cgatgcgggt
     6841 gttgatacaa aacaattaga agggcaaagc gatctcggct ttgccggttt tcgcgtgttt
     6901 aaagcccccg aactggcgcg ccgtgatgta gtatcatttc tcggcgcgag ttatttccgc
     6961 gccgttgatg atacatatca atacggtttg tcggcccgcg gcctggcgat cgacacttac
     7021 accgacagta aagaagagtt ccccgacttt accgccttct ggtttgatac ggtaaaaccg
     7081 ggggcaacta cctttaccgt ttatgcgttg ctcgatagcg ccagcattac tggtgcctat
     7141 aagttcacta tccattgtga gaaaagtcag gtgattatgg atgtggaaaa tcacctgtat
     7201 gcgcgcaaag acattaaaca gctgggcatt gcgccgatga ccagtatgtt cagctgcggt
     7261 actaatgaac gtcggatgtg cgatacaatt catccgcaaa ttcatgactc tgatcgtctg
     7321 tccatgtggc ggggcaacgg cgagtggatt tgccgtccgc tgaataatcc gcaaaaattg
     7381 cagttcaatg cttacaccga caacaacccg aaagggtttg gtttattgca actggatcgt
     7441 gacttctccc attatcagga cattatgggc tggtataaca aacgcccaag tctgtgggtg
     7501 gaaccgcgta acaagtgggg taagggcacc atcggcctga tggaaatccc aacaacgggc
     7561 gaaacgctgg ataacattgt ctgtttctgg cagccagaaa aagctgtaaa agcaggtgat
     7621 gagtttgcat tccagtatcg tctgtactgg agtgcgcaac cgcctgttca ttgcccatta
     7681 gcgcgcgtta tggcgacgcg taccggcatg ggcggtttcc cggaaggttg ggcgccaggt
     7741 gaacactatc ccgaaaaatg ggcgcgtcgt tttgccgtcg atttcgttgg tggtgatctg
     7801 aaagctgccg cgccaaaagg cattgagccg gtgattacgc tttccagtgg ggaagcgaag
     7861 caaatcgaaa ttctctatat tgaacccatc gatggttatc gtattcagtt tgactggtat
     7921 ccgacttcgg actccactga tccggtcgat atgcggatgt atctacgttg tcagggggac
     7981 gctatcagtg aaacatggct gtatcagtat ttcccgccag cgccggataa acgtcagtat
     8041 gttgacgacc gcgtgatgag ttaatcgttt tttcttcggc accttcttcg ggaggtgccg
     8101 tctggttaaa cacgatcccg ctcgcatttt tccctaagtt aaatgagtaa tctgatggtg
     8161 tgtatttcag atacatcttg tcagccacta acagggagtg cgtatgtttc cagaataccg
     8221 agatttaata tcccgtctga aaaacgaaaa tcctcgcttt atgtccttgt tcgataaaca
     8281 caataaactt gatcatgaaa ttgccagaaa ggaaggtttc gacggtcgag ggtacaatgc
     8341 ggaagtggtc cgcatgaaaa aacaaaagct acagttaaaa gatgagatgc tcaaaatcct
     8401 gcagcaggag agcgtcaaag gggtgtaaac tttcctaagc cgccagccag gcggcttttt
     8461 taacaacagc atggattgac tggagataag atgactgaaa cgataaaagt aagcgaatca
     8521 cttgaattac atgctgttgc ggaaagtcac gtcacacctc tttatcagtt aatctgtaaa
     8581 aataaaacct ggttacagca gtcgctaaac tggccgcagt tcgttcagag tgaagaggac
     8641 acgcgaaaaa cggtgcaggg taatgtgatg ttgcatcaac gcggctatgc caaaatgttc
     8701 atgattttca aagaagatga acttatcggc gttatctcgt ttaatcgtat tgaaccactg
     8761 aataaaactg ctgaaatcgg ctactggctg gacgaatctc atcaggggca ggggatcatt
     8821 tctcaggcgc tgcaggcatt gattcatcat tacgctcagt ctggtgaact tagacgcttc
     8881 gtgatcaaat gtcgggtgga caatccgcaa agcaaccaga tcgctttgcg caatggtttt
     8941 atccttgaag gttgcctgaa acaggctgag ttcctgaatg atgcctatga tgatgtgaac
     9001 ttatacgcgc gtattatcga ttcataataa ccctgccagc ggcgttcgcg taatgcgctc
     9061 ctcgccgtta atgacttttg gaccacggac atgaaccgtg tcgccatcaa aggcttcaac
     9121 gacggcgtgg tcggttaact ccacatggtt ttcaattatc accgcacctg aaatacggct
     9181 ctcaccttgt attacaacgt gctcgtcgag cagaatcggt tcaccacgta ccacggcatt
     9241 tccgccaatc aagacatgat gttttaacac acaattacct tccacaatgg catattccgc
     9301 cacctgcgaa ctgtaatgaa tcgtcggaat ggcatcttct tctatgccag ctttcacctg
     9361 cgcgtgaccg tagactttag cgcaatcgca tagccagatg ttgttctctt cattaccttc
     9421 aacactggca aaatcaaaaa cttcggcgcg atgttcaata aaagcatacc ggacgacggc
     9481 atcgccataa atttgtgcct ggtggacaat acgcgaggcg ctaacccttg cgcgatcata
     9541 aatttggagc aggagttgat ggtcgggcgt taagccttgt gcggcgacga tcatagaatg
     9601 ttgatcaatt aaggcgtgac caaatattct acattgcccg taaactaatg agtcatgaat
     9661 ggtgacactg tcacttatat atgcaccctg actgatttca ctattatcga tccagacgtt
     9721 atccgttgcg taaacctctc cccacagcac gctggtgccg gtgatacgcg tattaccaga
     9781 aatcaccgtt ccgccgaacg caatagcatt ctggtcgtaa atccagcaat ttccttccta
     9841 tgctaacacc gtttcgcgat cgatccagcc gccagcggtt cctgctatca catcgttaaa
     9901 atcgctaatg gcgataatct gccgtaataa cacacttttt tagtgccatc ttcctgataa
     9961 ctaaaggctc gctgttcttc acttagacga tatttgcgca tagcgttttc tcacaggtga
    10021 cttactataa ccgtagcaaa ttctgcggct ctggctatgc tcgagaaaat tccataaaat
    10081 gcatttcaaa tatactttat aaattaaaca aaatgagtaa gaagatgcag agcgataaag
    10141 tgctcaattt gccggcaggc tactttggta ttgtgttggg gacgataggg atgggatttg
    10201 cctggcgcta tgccagccag gtttggcagg tcagccactg gttaggggat gggctggtga
    10261 ttctggcgat gatcatctgg ggattattaa ctagcgcatt tattacccga ctcatacgct
    10321 ttccgcatag cgtgctggcg gaagttcgcc atccagtgct gagcagtttt gtgagtttgt
    10381 ttccggcaac gacgatgctg gtggcgattg gttttgttcc gtggtttcgc ccactggcgg
    10441 tgtgcctgtt cagttttggt gtcgtggttc agttggctta tgccgcctgg caaactgcgg
    10501 gattatggcg cggatctcac cctgaagaag ctaccatgcc tggactgtat ctgccgacag
    10561 ttgccaacaa ctttatcagc gcaatggcct gtggtgcgtt gggctacacc gacgccggtc
    10621 tggtgttttt aggcgcaggc gttttctcat ggctaagcct tgaaccggtg atcttgcagc
    10681 gtctgcgcag ttcgggagaa ttacccacgg cactgcggac atcactcggc attcagctcg
    10741 ctcctgcgct ggtggcctgt agtgcttggt tgagcgtcaa cggcggcgag ggtgatacgc
    10801 tggcgaaaat gctttttggt tatggactgc tgcaactgct gtttatgcta cgtctgatgc
    10861 catggtatct ctcccagcca tttaatgctt cattctggag tttctcgttc ggcgtatctg
    10921 cactggcaac caccggtttg catctgggga gtggcagcga aaatggattt ttccatacgc
    10981 tggcggtacc gctgtttatc tttaccaatt ttattattgc aatactgctc atccgtactt
    11041 ttgcgcttct gatgcaggga aaattgttag tcagaaccga gcgcgctgtt ttaatgaaag
    11101 cagaggacaa agaatgatca ttcgtgacga aaactatttt actgataaat atgaattaac
    11161 ccgcacacac tctgaagtac tggaagcggt gaaagtggtt aaaccgggta aaacgctgga
    11221 tctgggctgt ggcaatggtc gtaacagtct ttacctggca gccaatggtt atgatgttga
    11281 cgcatgggat aaaaatgcca tgagtatcgc caatgtagag cgcattaaat ccattgaaaa
    11341 tctggataat ttacacaccc gagtcgttga tctgaataac ctcacatttg atagacagta
    11401 cgattttatt ctttcgactg tggtactgat gttccttgag gctaaaacca tcccagggct
    11461 gattgccaat atgcaacgtt gcactaaact tggtggttac aacctgattg tggcggcgat
    11521 ggataccgct gattatccat gtaccgtcgg cttcccgttt gccttcaaag agggagaatt
    11581 acgtcgatat tacgaaggct gggagagggt gaaatacaat gaagacgtcg gcgagctgca
    11641 ccgcaccgac gccaacggta atcgtattaa actgcgtttc gccacgatgc tggcacgtaa
    11701 aaaatgaccc gctaagaata aggcgcgtga aaattcccca cgctgagatg atttactgtt
    11761 cttcttttcg gtaagcatat tttttatcga agggatgtga aattaatcac agtagtcgaa
    11821 gttttttagc agcttaactt actgaaattt aactactgat gattgactta gccccttttt
    11881 cggcattgac tatgtcgtct gaaaaggggc tgaaaaattt attttcacca acactttttt
    11941 tgccacaaca cgaagcggcg ctttttgcta taacttaaaa agtaatataa tcatctcagg
    12001 aaactattca tgcgtaccac atcatttgcg aaagttgcag ctttatgcgg cttgttggct
    12061 ctgtctggtt gtgcctctaa aatcacccag ccagataaat attctggttt t
//
LOCUS       DAJTNA010000122        12040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTNA010000122 DAJTNA010000000
VERSION     DAJTNA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12040
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.123"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..413)
                     /locus_tag="OO161_002833"
     CDS             complement(<1..413)
                     /locus_tag="OO161_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5463833.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALTTQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVTKAGYIALAPDGLSSVG"
     gene            569..1609
                     /gene="gpr"
                     /locus_tag="OO161_002834"
     CDS             569..1609
                     /gene="gpr"
                     /locus_tag="OO161_002834"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5463834.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1649..2143)
                     /locus_tag="OO161_002835"
     CDS             complement(1649..2143)
                     /locus_tag="OO161_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5463835.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2334..3218
                     /gene="yghA"
                     /locus_tag="OO161_002836"
     CDS             2334..3218
                     /gene="yghA"
                     /locus_tag="OO161_002836"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5463836.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVTGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3490..3915)
                     /gene="exbD"
                     /locus_tag="OO161_002837"
     CDS             complement(3490..3915)
                     /gene="exbD"
                     /locus_tag="OO161_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5463837.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3922..4656)
                     /gene="exbB"
                     /locus_tag="OO161_002838"
     CDS             complement(3922..4656)
                     /gene="exbB"
                     /locus_tag="OO161_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5463838.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4908..6095
                     /gene="metC"
                     /locus_tag="OO161_002839"
     CDS             4908..6095
                     /gene="metC"
                     /locus_tag="OO161_002839"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5463839.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6235..6894
                     /locus_tag="OO161_002840"
     CDS             6235..6894
                     /locus_tag="OO161_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5463840.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTITILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6934..7833)
                     /gene="yqhC"
                     /locus_tag="OO161_002841"
     CDS             complement(6934..7833)
                     /gene="yqhC"
                     /locus_tag="OO161_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5463841.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8027..9190
                     /gene="yqhD"
                     /locus_tag="OO161_002842"
     CDS             8027..9190
                     /gene="yqhD"
                     /locus_tag="OO161_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5463842.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPNALKKPENYDVRANVMWAATQALNCLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9295..10122
                     /gene="dkgA"
                     /locus_tag="OO161_002843"
                     /pseudo
     CDS             9295..10122
                     /gene="dkgA"
                     /locus_tag="OO161_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
     gene            10322..11248
                     /locus_tag="OO161_002844"
     CDS             10322..11248
                     /locus_tag="OO161_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5463843.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11299..11556
                     /gene="yqhH"
                     /locus_tag="OO161_002845"
     CDS             11299..11556
                     /gene="yqhH"
                     /locus_tag="OO161_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5463844.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            11635..>12040
                     /locus_tag="OO161_002846"
     CDS             11635..>12040
                     /locus_tag="OO161_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463845.1"
                     /translation="MRRWHTCFFILLFCGFSGNVLCKESINEAIERLYQPLTSNDDIP
                     ITDEFSLDLRSNMSATPGKMTPWLNNNLLCRCVDDAFVSAEIIQSTHLNGGRTHVLLQ
                     LVIKQNRTDIHRLLGLMLVRENDNHRWLIEDID"
BASE COUNT         3115 a         3061 c         2999 g         2865 t
ORIGIN      
        1 ccaacggaac ttaagccgtc aggtgccagg gcgatatacc ccgccttcgt cactcgccgt
       61 gccacatctt cgatatacgg attcagtcca cgattctcat gcaccaccac cacggctggc
      121 gttttgccgc tcatttttgc gggtttcacc agataacccc gtacctcgcc gtgaccattt
      181 ggcgaaggat acgtgatgta ctcagcaaca atctccgggt cggtaaactc tacctgagtc
      241 gtcagcgcat aatttggctt gagcaaatca aacaacgcta atgccgtcat cccgcccacc
      301 gcatacttcg ccgcaaggtt gaggaactca cgtttcgaaa ttttcccgtg agcgtaatag
      361 tcgtagtaat ccaacaactc ttgtgggaaa tctttggcgg ttaaacgcgg catcgttgca
      421 ttcctcaact ggtgtttttt aagcaaagca taagcgcgtg tttttgccca gtttttcgtc
      481 actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt
      541 cgaaattgaa cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg
      601 gcagatgcag taccgctatt gcggaaaaag tggtttacgc ctgcccgcgt tatcgctcgg
      661 tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa
      721 agcgtttgat ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg
      781 aagcgcagaa gagaactttg gtcgcctgct gcgggaggat tttgccgctt atcgcgatga
      841 actgattatc tctaccaagg ctggctacga tatgtggccc ggcccttacg gctctggcgg
      901 ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta
      961 tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc
     1021 tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc
     1081 gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgcttttaat
     1141 tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac
     1201 cctgcaaaat aacggcgtgg gctgtattgc ctttactcct ctggctcagg gattgctgac
     1261 cggaaaatat ctcaacggca ttccgcaaga ttcacggatg catcgtgaag ggaataaagt
     1321 tcgtggtctg acgccgaaaa tgcttaccga agccaacctc aacagcctgc gcttattgaa
     1381 tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa
     1441 agatgatcgc gtgacgtcgg tattgattgg tgtcagccgc gcggagcaac tagaggagaa
     1501 cgtgcaggcg ctgaataatc tgacatttag caccgaggag ctggcgcaga ttgatcagca
     1561 tatcgccgat ggcgagctga atctgtggca ggcgtcttcc gataaatgac ctgttaataa
     1621 cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat
     1681 agcccatcac aaatgcagag agcacaaacg tcagatggat aatgacgtac cacatcagtt
     1741 tgttatccgg gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg
     1801 ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccagt
     1861 tcagcttctc tttgttctcg gagatatcca gctgcgagac gaaattctca taaccggaaa
     1921 acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga
     1981 ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga
     2041 agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca
     2101 gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag
     2161 ttaaaacggg caacagtata tcgcaattga gtaaacagca cgcaacagca aatcaacata
     2221 tacgttaaac ctttgtcgca taattctgag gtttagggat cttcctggtt cccttatcaa
     2281 cgcatcgaga gcgtctaccc ttaaaagacg ttgaacctga agggagaaaa acaatgtctc
     2341 atttaaaaga cccgactacg cagtattaca ctggtgaata tcccaaacag aaacaaccga
     2401 cgccaggcat ccaagcgaag atgacaccgg taccggattg cggcgagaaa acctatgttg
     2461 gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc
     2521 gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg
     2581 tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg
     2641 ttctgctgcc aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc
     2701 acaaggcgtt aggcgggctg gatattatgg cgctggtcac cgggaaacag gttgccattc
     2761 cggatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg
     2821 cgctgttctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtatcatca
     2881 ccacttcgtc aatccaggca taccagccaa gtccgcattt actggactat gcggctacga
     2941 aggcggcgat tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc
     3001 gggtgaatat tgtcgcgcca ggccctatct ggacggcact gcaaatttcc ggcggacaaa
     3061 cgcaggataa gatcccgcag tttggtcagc aaacgccgat gaagcgtgcg gggcaaccgg
     3121 cggaactggc ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag
     3181 aagtgcacgg tgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag
     3241 caaccgggca tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct
     3301 acaaacgcat gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc
     3361 atcaggcatt gagcacctaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaacg
     3421 cacgaagatt caacatgttg caatagattt tgtaggcctg atgagcgtag cgcatcaggc
     3481 aattctactt tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc
     3541 cgcctgatgc agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttatccgc
     3601 tcggaagaag atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat
     3661 tgtttcatcg gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag
     3721 ataaaccggt ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt
     3781 cacatctacc gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac
     3841 gtcgataaac ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt
     3901 aagatgcatt gccatcggat attatcctgc gcgtaatttt tgtgcgacac gaaccggatg
     3961 cgcagcggcg ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc
     4021 aacatcaccc agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac
     4081 gaccgcagga atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc
     4141 cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat
     4201 gatgccccag accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag
     4261 atagccgtta ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa
     4321 gctggtacgt tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt
     4381 ctgcgcttca ttgaacaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc
     4441 gttggcctgg tttaaggaac gcgcttcagc cagcagttgc tgctcgcgct ttaggcgacg
     4501 cttctgattg aagaactcta cgctcttact gaagaagatt gcccaggtga ctacggaggc
     4561 caaaataagc ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca
     4621 gacggaaagg tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca
     4681 caaaatctgc gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat
     4741 ttgcatactg ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca
     4801 gcacgccatt tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata
     4861 ttcatgctag tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa
     4921 agcttgatac tcaactggtg aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa
     4981 atagcgtgat tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg
     5041 cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt
     5101 tttccttaca acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct
     5161 gcggggcggc agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt
     5221 tgatgaccaa cactgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac
     5281 tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc
     5341 agccaaacac taaaatcgtg tttctggaat cgccaggctc catcaccatg gaagtccacg
     5401 acgttccggc gattgttgcc gccgtacgca gtgtggtgcc ggatgccatc attatgatcg
     5461 acaacacctg ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta
     5521 ttcaagccgc caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt
     5581 gcaatgcccg ttgctgggag cagctacggg aaaacgccta tctgatgggc cagatggtcg
     5641 atgccgatac cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc
     5701 aacatcatga aagcagtctg aaagtggctg aatggctggc agaacatccg caagtagcgc
     5761 gagttaacca ccctgctctg cctggcagta aaggacacga attctggaaa cgagacttta
     5821 caggtagcag cgggctgttt tcctttgtgc ttaagaaaaa actcagtaat gaagagctgg
     5881 cgaactatct ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat
     5941 cgttgatcct ggcgaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg
     6001 attttagcgg gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg
     6061 ccgatctgga cgccggtttt gcgcgaattg tataacattg ccacttttgg acaattttgc
     6121 agacatttaa ttgtgaaaag tcttaaattg ttgcgtcccg gatcaaggcg tcccggacga
     6181 atcaggagta caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct
     6241 gttattcaag atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct
     6301 catattgtta gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg
     6361 ctgcccgcct catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc
     6421 cagggggtta tggattttct gcctacgatt acgattctga ccgccgcagc aagtctgggc
     6481 tgctggctaa gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg
     6541 ctggcacagc ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt
     6601 ctgctggcgc tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg
     6661 gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg
     6721 ctgtgggtca gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa
     6781 cgccatgaag atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct
     6841 ggcttgttag gcatgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt
     6901 tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct
     6961 tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga
     7021 tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat
     7081 catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt
     7141 gacagactta aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg
     7201 ctcgacgctc aggttttcgg tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag
     7261 actgaagtga gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag
     7321 cacgtagtac agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg
     7381 ctccatcacg tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga
     7441 gttaatcccg ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa
     7501 cagttcctgt aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga
     7561 cgtggcatag gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc
     7621 atcataacga aacacgcgtt cattgatata accgatttta tgcccggaaa agagaattat
     7681 gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg
     7741 cacatcgggc aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc
     7801 cgccagcaag cggcaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg
     7861 gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga
     7921 cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg
     7981 cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa
     8041 tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca
     8101 aattcctcac gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg
     8161 cgttctcgat caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat
     8221 tgagccaaac ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa
     8281 agtgactttc ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc
     8341 cgcagcggct aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa
     8401 agagattaaa agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga
     8461 atccaacgca ggcgcggtga tctcccgtaa aaccactggc gacaagcagg cgttccattc
     8521 tgcccatgtt cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc
     8581 gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac
     8641 caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctgat
     8701 cgaagatggt ccgaacgccc tgaaaaagcc agaaaactac gatgtgcgcg ccaacgtcat
     8761 gtgggcggca acgcaggcgc tgaactgttt gattggcgct ggcgtaccgc aggactgggc
     8821 aacgcatatg ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact
     8881 ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgat accaagcgcg ctaagctgct
     8941 gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc
     9001 cgcgattgcc gcaacccgca atttctttga gcaattaggc gtgccgaccc acctctccga
     9061 ctacggtctg gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat
     9121 gacccaactg ggcgaaaatc atgacattac gttggatgtc agccgccgta tatacgaagc
     9181 cgcccgctaa gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag
     9241 ttcacaacac ctcaccggag cctgctccgg tgaggtcata taaaggagga acgtatggct
     9301 aatccaaccg ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc
     9361 tggcaagcaa gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat
     9421 cgctcgattg ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa
     9481 aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac
     9541 aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac
     9601 ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc
     9661 atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc
     9721 catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa
     9781 cttcatccgc tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag
     9841 accgaatcct agagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt
     9901 cgcgatctgg cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg
     9961 gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt
    10021 gatgtctggg atttccgtct cgacaaagac gaactcggcg aaattgcaaa actcgatcag
    10081 ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct aacatgcaaa ttctcccggt
    10141 ggcggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc
    10201 gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga
    10261 agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca
    10321 gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcgcagcc
    10381 tccttcacag accgtagaac aaacagtccg gcagatttat cagaactata aatcagatgc
    10441 cagtacgcct tatttcggtg aaaccggaga gcgggcgata acttcagcgc gtattcaaca
    10501 ggcgcttacc ctgaacgaca atcttacgct gccgggcaat attggctggc tggattatga
    10561 tccggtttgt gattgtcagg attttggcga tctggtgcta gaaagcgttg cgataaccca
    10621 aactgacgcc gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga
    10681 aaagaccacg cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgacgatat
    10741 tgtcagcaat catggcagcg tcttacaagc agttaatagc gagaatgaaa aaacgctggc
    10801 cgctttagct tcgttgcaaa aagaacagcc ggaagccttt gttgccgaac tctttgaaca
    10861 tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt
    10921 taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat
    10981 agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg
    11041 cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt
    11101 tacgctgacc aatggcaaca acgaagagca agaactggtt ttacagcggc gcgaaggcaa
    11161 gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc
    11221 aaaaactgcc gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata
    11281 aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc
    11341 tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc agctggcgag caatgtgcaa
    11401 acattaaatg ccaaaatcgc ccggcttgag caggatatga aagcactacg cccacaaatc
    11461 tatgctgcca aatccgaagc taacagagcc aatacgcgtc ttgatgcaca ggactatttt
    11521 gattgcctgc gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gcagcatgtc
    11581 agttgccggg ttataggctg tttgctaaaa aaagccaaac ataaggacat gattatgcga
    11641 cgctggcaca cctgtttttt tattctgctg ttctgtggct tcagcggaaa cgtgttgtgc
    11701 aaagaatcga ttaatgaagc gatagaacgt ttgtaccaac cattgaccag taatgacgat
    11761 ataccaataa ccgatgaatt cagtctcgat ttgcgaagta atatgagcgc tactcctggc
    11821 aaaatgacac cctggctgaa taataatctt ttgtgtcgct gtgtcgatga cgctttcgtt
    11881 tctgcagaaa taatccaaag cacacaccta aatggcggcc gaacgcatgt actgttgcag
    11941 ctggttatca aacaaaatcg cacagatatc caccggttac tggggctaat gctggttcgt
    12001 gaaaatgata atcatcgttg gctaatagaa gacatcgatg
//
LOCUS       DAJTNA010000123        11875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTNA010000123 DAJTNA010000000
VERSION     DAJTNA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11875
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.124"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            216..380
                     /locus_tag="OO161_002847"
     CDS             216..380
                     /locus_tag="OO161_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5463846.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            383..550
                     /locus_tag="OO161_002848"
     CDS             383..550
                     /locus_tag="OO161_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463847.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            664..759
                     /locus_tag="OO161_002849"
     CDS             664..759
                     /locus_tag="OO161_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463848.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            923..3598
                     /gene="acnA"
                     /locus_tag="OO161_002850"
     CDS             923..3598
                     /gene="acnA"
                     /locus_tag="OO161_002850"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5463849.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGSGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWTAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGKEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3662..4252)
                     /gene="ribA"
                     /locus_tag="OO161_002851"
     CDS             complement(3662..4252)
                     /gene="ribA"
                     /locus_tag="OO161_002851"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5463850.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4422..5186
                     /gene="pgpB"
                     /locus_tag="OO161_002852"
     CDS             4422..5186
                     /gene="pgpB"
                     /locus_tag="OO161_002852"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5463851.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5335..5643
                     /gene="lapA"
                     /locus_tag="OO161_002853"
     CDS             5335..5643
                     /gene="lapA"
                     /locus_tag="OO161_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5463852.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5650..6819
                     /gene="lapB"
                     /locus_tag="OO161_002854"
     CDS             5650..6819
                     /gene="lapB"
                     /locus_tag="OO161_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5463853.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            7012..7749
                     /gene="pyrF"
                     /locus_tag="OO161_002855"
     CDS             7012..7749
                     /gene="pyrF"
                     /locus_tag="OO161_002855"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5463854.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7749..8075
                     /gene="yciH"
                     /locus_tag="OO161_002856"
     CDS             7749..8075
                     /gene="yciH"
                     /locus_tag="OO161_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5463855.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8201..8419)
                     /gene="osmB"
                     /locus_tag="OO161_002857"
     CDS             complement(8201..8419)
                     /gene="osmB"
                     /locus_tag="OO161_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5463856.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8688..9437)
                     /locus_tag="OO161_002858"
     CDS             complement(8688..9437)
                     /locus_tag="OO161_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5463857.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(9527..9700)
                     /locus_tag="OO161_002859"
     CDS             complement(9527..9700)
                     /locus_tag="OO161_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463858.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(9847..11796)
                     /gene="pdeR"
                     /locus_tag="OO161_002860"
                     /pseudo
     CDS             complement(9847..11796)
                     /gene="pdeR"
                     /locus_tag="OO161_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016150331.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
BASE COUNT         3017 a         2800 c         3017 g         3041 t
ORIGIN      
        1 tgcacaatat ttttatccct catatttata gggtaaatta cctgtaaagt agtgtctaat
       61 tacccgcctc gcaaattcca gttttcaaat atctatttca ggtcaaaaga ttgaattatt
      121 cactggagac gattcgtaaa ttcgctggat ttttgactaa agtttcttta ggatttgtct
      181 catcgtatga ttaattacac caatcgttca acgtcttgct gataagtgat ggcgattata
      241 tgaggttagc aatgccttct ggaaatcagg aaccccgccg cgatcctgaa ttaaaacgta
      301 aagcctggct ggcggttttt cttggttctg cacttttctg ggtggttgtc gcactgctga
      361 tttggaaagt gtggggataa ctatggtcgg tcaggagcaa ctggagtcgt caccattatg
      421 ccagcatagt gacaatgaaa cagaaacgaa acgggaatgt tccgtcgtta ttccagacga
      481 ctggcaacta acatcgcagc agcaagcctt tatagaactg tttgctgaag atgatcagcc
      541 gaaacaataa ttatcatcat tcttattacc catttttaat gaattaaagg gcttttaata
      601 caccgcagca ataacagctt gagttatctc aacacaaaat aataaccgtt aagggtgtag
      661 gctatgatca acacaaatat gaaatattgg tcctggatgg gcgcgttttc tctgtcgatg
      721 ctcttctggg ccgaactcct ctggatcatt actcactgat ccttgacccc gctgcggcgg
      781 ggttgtcatt tgctttgcca caaggtttct cctcttttat caatttgggt tgttatcaaa
      841 tcgttacgcg atgtttgtgt tatctttaat attcaccctg aagagaatca gggcttcgca
      901 accctgtcat taaggaggag ctatgtcgtc aaccctacga gaagccagta aggacacgtt
      961 gcaggccaaa gataaaactt accactacta cagcctgccg cttgctgcta aatcactggg
     1021 cgatatcacc cgtctaccca agtcactcaa agttttgctc gaaaacctgc tgcgctggca
     1081 ggatggtaac tcggttaccg aagaggatat ccacgcgctg gcaggatggc tgaaaaatgc
     1141 ccatgctgac cgtgaaattg cctaccgccc ggcaagggtg ctgatgcagg actttaccgg
     1201 cgtacctgcc gttgttgatc tggcggcaat gcgcgaagcg gttaaacgcc tcggcggcga
     1261 tactgcaaag gttaacccgc tctcaccggt cgacctggtc attgaccact cggtgaccgt
     1321 cgatcgtttt ggtgatgatg aggcatttga agaaaacgta cgcctggaaa tggagcgcaa
     1381 ccacgaacgt tatgtgttcc tgaaatgggg aaagcaagcg ttcagtcggt ttagcgtcgt
     1441 gccgccaggc acaggcattt gccatcaggt taacctcgaa tatctcggca aagcagtgtg
     1501 gagtgaattg caggacggtg aatggattgc ttatccggat acactcgttg gtactgactc
     1561 gcacaccact atgatcaacg gccttggcgt gctggggtcg ggcgtgggtg ggatcgaagc
     1621 agaagccgca atgttaggcc agccggtttc catgcttatc ccggacgtag tgggctttaa
     1681 acttaccggt aaattacgtg aaggtattac cgccacagac ctggttctca ctgttaccca
     1741 aatgctgcgc aaacatggcg tggtggggaa attcgtcgaa ttttatggtg atggtctgga
     1801 ttcactaccg ctggcagatc gcgccaccat tgccaatatg tcgccagaat atggtgccac
     1861 ctgtggcttc ttcccaatcg atgctgtaac cctcgattac atgcgtttaa gcgggcgcag
     1921 cgaagatcag gttgagttgg tcgaaaaata tgccaaagcg cagggcatgt ggcgtaaccc
     1981 gggcgatgaa ccgattttta ccagtacgtt agaactggat atgaatgacg ttgaagcgag
     2041 cctggcaggg ccaaaacgcc cacaggatcg cgttgcactg cccgatgtac caaaagcatt
     2101 tgccgccagt aacgaactgg aagtgaatgc cacgcataaa gatcgccagc cggtcgatta
     2161 tgttatgaac ggacatcagt atcagttacc tgatggcgct gtggtcattg ctgcgataac
     2221 ctcgtgcacc aacacctcta acccaagtgt gctgatggcc gcaggcttgc tggcgaaaaa
     2281 agccgtaact ctgggcctca agcggcaacc gtgggtcaaa gcgtcgctgg caccgggttc
     2341 gaaagtcgtt tctgattatc tggcaaaagc gaaactgaca ccgtatctcg acgaactggg
     2401 gtttaacctt gtgggatacg gttgtaccac ctgtattggt aactctgggc cgctgcccga
     2461 tcctatcgaa acggcaatca aaaaaggcga tttaaccgtc ggtgcggtgc tgtccggcaa
     2521 ccgtaacttt gaaggccgta tccatccgct ggttaaaact aactggctgg cctcgccgcc
     2581 gctggtggtt gcctatgcgc tggcgggaaa tatgaatatc aacctggctg ctgagccgat
     2641 tggtcatgat cgtaaaggcg acccggttta tctgaaagat atctggccat cggcacaaga
     2701 aattgcccgt gcggtagaac aagtctccac agaaatgttc cgtaaagagt acgcagaagt
     2761 ttttgaaggc acagcagagt ggaaggaaat taacgtcaca cgatccgata cctacggttg
     2821 gcaggaggat tcaacctata ttcgcttatc gcctttcttt gatgaaatgc aggcaacacc
     2881 agcacctgtg gaagatattc acggtgcgcg gatcctcgca atgctggggg attcagtcac
     2941 cactgaccat atctctccgg cgggcagtat taagcccgac agcccggcgg gtcgatatct
     3001 acaaggtcgg ggtgttgagc gaaaagactt taactcctac ggttcgcggc gtggtaacca
     3061 tgaagtgatg atgcgcggca ccttcgccaa tattcgcatc cgtaatgaaa tggtgcctgg
     3121 cgttgaaggg gggatgacgc ggcatttacc tgacagcgat gttgtctcta tctatgatgc
     3181 cgcgatgcgc tataagcagg agcaaacgcc gctggcggtg attgccggga aagagtatgg
     3241 atcaggctcc agccgtgact ggacggcaaa aggtccgcgt ctgcttggta ttcgtgtggt
     3301 gattgccgaa tcgtttgaac gaattcaccg ttcgaattta attggcatgg gcatcttgcc
     3361 gctggaattt ccgcaaggcg tgacgcgtaa aacgttaggg ctaaccggga aagagaagat
     3421 tgatattggc gacctgcaaa atctacaacc cggcgcgacg gttccggtga cgcttacgcg
     3481 cgcggatggt agccaggaag tcgtaccctg ccgttgtcgt atcgacaccg cgacggagtt
     3541 gacctactac cagaacgacg gcattttgca ttatgtcatt cgtaatatgc tgaagtaaca
     3601 agtatttgct tgccggttat tttgcttccg gcaagcaaat taattccaca atgcgagagg
     3661 gttatttgtt cagcaaatga cccattttct cggctttggt atcgagataa tgttcgttat
     3721 tggggttacg acctacaatc aatggtacgc gttcaacaat attaatccct gcttcggtca
     3781 gaatttcgac ttttttcggg ttattggtta acaagcggac ttcattgacg ccaaggagtt
     3841 tgaacatatc agcgcaaaga gtgaagtcgc gctcatcagc ggcgaagcct aactggtggt
     3901 tagcctctac ggtatcgtaa ccttgatcct gcagtgcgta agcgcggatt ttattcagca
     3961 gaccaatgtt acgaccttcc tgacggtgat acagcaaaat accacggcct tcctcggcaa
     4021 tttgcgtcaa tgccgcttcg agctggaagc cacaatcgca gcgcaagctg aacagggcgt
     4081 caccggtcag acattcggaa tggacgcgcg caagtaccgg ggtatgcccg gaaatatcgc
     4141 catagactag cgcgacatga tcgtgtccgg ttgccagttc ttcaaatccc accatcagga
     4201 aatcgcccca tggggttggc agtttggctt ctgccacacg tttaagctgc atgaaattct
     4261 ccagataatg ctggttctgt attggcttat tttgccataa cgagaagggg ttcacctaat
     4321 cacgacgcgt cgatcgttca cggaatggca caaatctgtc aatttttcct ggaactggcg
     4381 ttttcagtta tgattgtggg acttatcaaa aaggagaggc catgcgttcg attgccagac
     4441 gtaccgcagt gggagctgca ctattgcttg tcatgccagt agccgtatgg atttctggct
     4501 ggcgttggca acctggagaa caaagttggc tactaaaagc ggctttttgg gttactgaaa
     4561 ctgtcaccca gccctggggc gtcattacac atttgatttt attcggctgg tttctctggt
     4621 gtctgcgttt tcgcattaag gctgcctttg tattatttgc cattctggcg gccgcaatcc
     4681 ttgtgggaca aggcgttaaa tcctggatca aagacaaagt ccaggaacca cgaccttttg
     4741 ttatctggct ggaaaaaaca catcatattc cggttgatga gttctacact ttaaagcgag
     4801 cagaacgcgg aaatctagtg aaagaacagt tggctgaaga gaaaaatatc ccacaatatt
     4861 tgcgttcaca ctggcagaaa gagacggggt ttgcctttcc ttccggtcac acgatgtttg
     4921 ctgccagttg ggcactgctg gccgttggtt tgctgtggcc gcgtcggcga acgttaacca
     4981 ttgctatctt gctggtctgg gcaacgggag tcatgggaag ccgcctgctg ctcgggatgc
     5041 attggccacg cgatctggta gtagctacgt tgatttcgtg ggcgctggtg gcggtggcaa
     5101 cgtggcttgc gcaacgaatt tgtgggccat taacaccacc tgcggaagaa aatcgcgaaa
     5161 tagcgcaacg agaacaagaa agttaacgct ggttgatttt ccgaatttag cccttaaatc
     5221 atcaacaatg cgcgtggatg ccatttcgca gacggcgcga aaatggtact ttaaagggct
     5281 attgcggtaa gttgaccata atttattcgc tctaaccaca taacgggaag taatgtgaaa
     5341 tatttactca ttttcttact ggtgttagcg atcttcgtga tttcggtcac gttgggtgcg
     5401 cagaacgatc aacaggtgac gtttaattat ctgttagcgc aaggggagta ccgtatttcc
     5461 acattgctgg cggtattgtt tgctgcgggg tttgctatcg gttggttgat ttgtggcctg
     5521 ttctggctgc gagttcgtgt ttccctggcg cgcgctgaac gtaaaataaa gcgactggaa
     5581 aaccagcttt cacccgcgac tgacgtggct gtagtgccgc actcgtcagc ggcgaaggaa
     5641 taactttcta tgctggagtt gttgtttctg cttttgcctg tagccgctgc ctatggctgg
     5701 tatatgggcc gcagaagtgc gcaacaaaac aagcaagatg aagccaaccg cttgtcgcgt
     5761 gattacgtag cgggggttaa cttcctgctt agtaatcaac aggataaagc ggtagacctg
     5821 tttctcgata tgcttaaaga ggatacaggc accgttgaag cccaccttac gctcggaaac
     5881 ctgttccgtt cgcgtggcga agttgatcgc gctattcgca tccatcagac cctaatggaa
     5941 agcgcctcgc tgacctatga acagcgtctg ttggcgattc aacaactagg gcgtgattac
     6001 atggccgccg ggttatacga ccgcgcggaa gacatgttca atcagctgac cgatgaaact
     6061 gacttccgca ttggcgctct gcaacagttg ctacaaatct accaggctac cagtgagtgg
     6121 cagaaagcaa ttgatgttgc cgaacgcctg gtgaagctgg gtaaagataa acagcgcgtc
     6181 gaaattgccc atttctactg tgagttagcc ctgcagcata tggccagcga cgatctcgat
     6241 cgtgccatga ccttgctaaa aaaaggggcg gcggcagata aaaacagcgc ccgcgtatcc
     6301 atcatgatgg gacgcgtgtt tatggcgaaa ggagaatacg ccaaagccgt cgaaagtttg
     6361 caacgcgtga tatcccagga cagagaactg gtcagcgaaa cgctggaaat gttgcaaacc
     6421 tgctatcagc agttgggtaa aactgccgaa tgggcagaat tcctgcaacg cgcggtggaa
     6481 gagaacaccg gtgccgatgc tgaattgatg cttgctgata tcatcgaagc gcgcgacggt
     6541 agtgaggccg cacaggtcta tattacgcgc cagcttcagc gtcatccgac catgcgtgtg
     6601 ttccataagt taatggatta ccacttaaat gaagcggaag aagggcgtgc caaagagagc
     6661 ctgatggtgc tgcgtgacat ggttggcgaa aaggtacgta gtaagcctcg ttatcgctgc
     6721 cagaaatgtg gttttaccgc atacaccctc tactggcatt gtccgtcttg tcgggcctgg
     6781 tcaaccatta aaccgattcg cggtcttgat ggcctgtaat ttttaaaaaa atccgacttt
     6841 agttacaaca tactagttat tggtgttcca ttgtgctccg gcaacgacgg agcagaaaaa
     6901 gcctgccagg ggaaaaatcg caactgttaa ttttttattt ccacgggtag aatgctcgcc
     6961 gtttacctgt ttcgcgccac ttccggtgcc catcatcaag aaggtctggt catgacgtta
     7021 actgcttcat cttcttcccg cgctgttacg aattctcctg tggttgttgc ccttgattat
     7081 cataatcgtg atgacgcgct gtcctttgtc gacaagatcg acccacgcga ttgtcgtctg
     7141 aaggtcggca aagagatgtt tacattgttt gggccacagt ttgtgcgcga acttcaacag
     7201 cgtggttttg atatctttct tgacctgaaa ttccacgata ttcctaacac tgcagcgcac
     7261 gctgtcgctg ctgcagctga cttaggcgtg tggatggtga atgttcatgc ctctggtggg
     7321 gcgcgtatga tgaccgcagc gcgtgaggca ctggttccgt ttggcaaaga tgcaccgctt
     7381 ttgattgctg taacagtgtt gaccagcatg gaagctagcg acctggccga tcttggcgtg
     7441 acactgtcac ctgcagatta tgcagaacgt ctggcggcac tgacgcaaaa atgtggcctt
     7501 gatggtgtgg tgtgttctgc tcaggaagct gtgcgcttta aacaggtatt cggtcaggag
     7561 ttcaaactgg ttacaccggg cattcgtccg caggggagtg atgctggtga ccagcgccgc
     7621 attatgacgc cagaacaggc gttggcggct ggtgttgatt atatggtgat tggtcgcccg
     7681 gtaacgcaat cggtagatcc agcgcaaaca ctgaaagcga tcaacgcctc tttacagcgg
     7741 agtgcatgat gagtgattcc aacagccgtc tggtctactc aacggaaacc ggacgtattg
     7801 atgagccaaa agcggcccca gtacgcccta aaggcgatgg tgtggtgcgt attcagcgtc
     7861 agaccagtgg acgtaaaggt aagggcgttt gcctgattac cggtatcgat ctcgatgatg
     7921 ccgaactgac aaaacttgca gcggaactga agaaaaaatg cggctgcggc ggagcagtta
     7981 aagatggagt tattgaaatc cagggcgata agcgtgattt attaaaatca ctacttgaag
     8041 cgaaagggat gaaggtaaaa ctcgcaggcg gttaacataa aaagccacgg atatatccgt
     8101 ggctttcgaa tattttactg tgtgtattat tcagttattt cgaactgaac aaaatcaagc
     8161 gtattataat tgcgagtcga taatcgacat ttatttacga ttatttaccg acctggtgac
     8221 caataacacc accgacagct gcaccaccta atgtacccaa cgtactgcca tcggtcagta
     8281 ctgcaccgcc taatgcccct gcacccgcgc cgattgcggt gttgcggtcc cgtttagacc
     8341 agttagaaca ggcactcaga gacattgcca aagtaattgc cagaacagcc gcggtcattt
     8401 ttttgctcgt tacaaacata atactctctc ctgaatttat gattcacgga agtaagctct
     8461 ctataactat aatagctaag aataagcctg gtgaaattat tccgtgaaat ctgctcgcgc
     8521 gcagggttat atcacgcagg tgataatgac ttcctgttat atcgctgata ataattttat
     8581 atcttaagcg tgttaataac aggtaaatag tcttaattat caaccaggaa tcatcttaga
     8641 gcggatgatt tgccaaactg caaatcatcc gtagagaagg gaaatggtta aatgtcaacg
     8701 acgtgaacgg tgagtttact gtgctcaaga tgcatcagat cgctggcgcg tattttcgaa
     8761 tcggtaatca cgcgattgaa tcgctcaacg ggaccgatgg agtatgaatg tacagcacca
     8821 aatttcgagc tgtcagtcag gactatcact tcgcactctt tttccagcac ggcattgacc
     8881 acatcggtac gcatcatatc gcgaccggtg aatccagttt caggttgcca gccatcaata
     8941 ccaataaatg ctttgctgaa atgcacctgc tggatgcact ggcgtgtcaa cgggccaacc
     9001 atactttcgc tttttttctg gtacacgcca ccgagcaaaa tgacttcaca aggcgcgtct
     9061 ttcagcaaat gcgcgatgta gctgctgacc gtgatgatag tgacattttt cttctgctcc
     9121 cccagagtcc gggcgagcag ggcattgctg ctgccatttt cgataaagat ggtttcgccc
     9181 ggttgaacca gtgacgcggc aaactcggcg agttcacgct tcagcgtata gttgctcatc
     9241 atacgggttt cgacgtcatc actatcaagc gaaactgcaa agccatgtgc acggcggagg
     9301 taactcagtt tttcgagggt gttgagatcc tggcgaatgg taacttcaga aactccagtg
     9361 gcttttgcca gatcggttac gctaacctga ccctggtcaa tgaccatctg tagaatagtt
     9421 tgttgtcggg aattcatcgc tgtaattctt ataacgttat aatactaaat aaaaaaatac
     9481 tgacgcgccg ggcatcctca aataggaaaa cctgaaggca cgattgttaa atctcccacg
     9541 gtgccttcgg gatgtctggc gtagattcag caacctggcg caagagttct gctttaagta
     9601 tctcgagatt atggatcgca gagtggtaat cgcctgccac aaggatatct aacacaatat
     9661 caatacgttc ggcaacgcgc tgggcatcaa attcagacat caaggatatc cttatagcaa
     9721 aagtggaaaa tgaataatgc aaaaattgcc gctaaaagag aagtgtttaa cagcaacggc
     9781 taattatcat ccaggaatac gatatataat gacgggatat agcgctaagt atatatattc
     9841 atctacttat gcgcgcttca gatagcgttt ataccagcgt tcgaaggcga cggcgggcat
     9901 cggtttggca aacaaaaatc cttgccgctc attgatcccg ttcttggtta aaaaagcatc
     9961 ttcctttgca ctctctacac cttcggcgat cacctgaaga ttcaatgcct gggccacagc
    10021 gacgatcgcc cggaccagtg actgcgagac aggttgtttg tgaatatctc gaacaaaaac
    10081 ctggtcaagt ttgatggcat cgatcggaaa gcgcgccagt tgcgaaagtg aagagtagcc
    10141 ggtaccaaaa tcatccagat gcacttgcgc acctagttgg ctaaattgtt gaataacaga
    10201 cagtgccagt tcatcattct caatcagaca actctctgtc agttcaacat ctatagggca
    10261 gtattcaaaa ttgagttcct ggagaacctg tttcagggcg gtgaaaatgg tttgatcggc
    10321 gagctgacgt gcagaaatat ttaccgccac tcgcaggttt atgcctttat cccgccactt
    10381 tgccacctgg cgtacgacat cgagaatcac ccaacggcct aaaggcacaa ttagccctga
    10441 ctcttcggcg taggaaatga agtccaacgg tggaatcaac ccacgttcag gtgactgcca
    10501 acgtactagt gcttccagac tgcgcacttc gccacgctag gtgattttcg gttgatagtg
    10561 aataaccaac tgatcgtttt ccagtgcttt acgcaagttg gtatccagcc agagatattc
    10621 aaatacccgt tgattcattt ctggggtaaa aacgcaaaat tgtcctcgtc cgccttcctt
    10681 cgctgtgtac attgctgtgt cggcgtgacg aataatagcc gtgctgtctg aaccatgttc
    10741 gggagagagt gcaataccta ctgaacagct ggtataaact tcaattaaac caatgcgaaa
    10801 ggggagccgt aagcgggtca aaattcgtga tgccattgct tccagcgcgc tttgtgaggt
    10861 gttggatgcc agtaccagaa actcatcccc acctggacgc gccaacacct ggtcatgttc
    10921 gagacagctt aaaatagcca atgacacgtc gcgtaataac tggtcaccaa acaaatgccc
    10981 ataggcgtcg ttgacctttt tgaaattatc caaatcaaga tacacaaccc caactttata
    11041 gttatctgca tgattaatag cgtgatcgat taaatcctgc attgcgttac gattcggcag
    11101 tccggtgata ctgtcggtat ttgccagaat acgcagtcgc tcctgagcgc ggcgctcttc
    11161 ggtaatgtcg gtgccggaac agattaaaaa aatctcgttt ttgccactgc cgctgtggac
    11221 aaatttattg cgaaacagaa acagccgctg gcctttacat gttggtatcc acagttcgac
    11281 ttcatatgca ttgccgcttc gaaaaaatac acggttattg cgcctggatg tcgcagcttc
    11341 acgacggctc ataaacagtt taaacacgct ttggccaatg acgtcgtgtt ctttcaaccc
    11401 agtgtaatct tcacataacc gattgaagcg ttggatattc ccccggctgt cgagaatcac
    11461 tattgcggaa tgtgcttcag aaactacctg ctcagcaaag gaaagcccgt gtgataaatc
    11521 acgagcaact gccggtgtgt catgccatgc agacgcgttg ccagcccatt cccgtttatt
    11581 gatttttcgt cctaccagat gcacagaaag atcgctctca tcgacggtca gactcatcat
    11641 caggctggag gtgatgaccg tcatttccct gatgcgagca gcctgttcgg cagataactg
    11701 caaagtacga tcaggttctg tggtttcggt ggtagaaaag cgcaaaacat cgctgctttc
    11761 cgtcaaccgc cagtatggat tgtgcgatcc gagaatgtgg actccctaac ggttttcatg
    11821 atgcatatcc tccggttaac agcggagtcc gcggttaaat aaaaggaaca acatg
//
LOCUS       DAJTNA010000124        11837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTNA010000124 DAJTNA010000000
VERSION     DAJTNA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.125"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..309)
                     /locus_tag="OO161_002861"
     CDS             complement(<1..309)
                     /locus_tag="OO161_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCR5463859.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     V"
     gene            complement(424..822)
                     /locus_tag="OO161_002862"
     CDS             complement(424..822)
                     /locus_tag="OO161_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5463860.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1062..3476)
                     /gene="gyrB"
                     /locus_tag="OO161_002863"
     CDS             complement(1062..3476)
                     /gene="gyrB"
                     /locus_tag="OO161_002863"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5463861.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3505..4578)
                     /gene="recF"
                     /locus_tag="OO161_002864"
     CDS             complement(3505..4578)
                     /gene="recF"
                     /locus_tag="OO161_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5463862.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4578..5678)
                     /gene="dnaN"
                     /locus_tag="OO161_002865"
     CDS             complement(4578..5678)
                     /gene="dnaN"
                     /locus_tag="OO161_002865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5463863.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5683..7086)
                     /gene="dnaA"
                     /locus_tag="OO161_002866"
     CDS             complement(5683..7086)
                     /gene="dnaA"
                     /locus_tag="OO161_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5463864.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7383..7463
                     /locus_tag="OO161_002867"
     CDS             7383..7463
                     /locus_tag="OO161_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463865.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7693..7833
                     /gene="rpmH"
                     /locus_tag="OO161_002868"
     CDS             7693..7833
                     /gene="rpmH"
                     /locus_tag="OO161_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5463866.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            7850..8209
                     /gene="rnpA"
                     /locus_tag="OO161_002869"
     CDS             7850..8209
                     /gene="rnpA"
                     /locus_tag="OO161_002869"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5463867.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8173..8430
                     /gene="yidD"
                     /locus_tag="OO161_002870"
     CDS             8173..8430
                     /gene="yidD"
                     /locus_tag="OO161_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5463868.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8433..10079
                     /gene="yidC"
                     /locus_tag="OO161_002871"
     CDS             8433..10079
                     /gene="yidC"
                     /locus_tag="OO161_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5463869.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10184..11548
                     /gene="mnmE"
                     /locus_tag="OO161_002872"
     CDS             10184..11548
                     /gene="mnmE"
                     /locus_tag="OO161_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5463870.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHVLIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            11790..>11837
                     /gene="tnaC"
                     /locus_tag="OO161_002873"
     CDS             11790..>11837
                     /gene="tnaC"
                     /locus_tag="OO161_002873"
                     /inference="COORDINATES: protein motif:HMM:NF019665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5463871.1"
                     /translation="MNILHICVTSKWFNID"
BASE COUNT         2704 a         3242 c         3061 g         2830 t
ORIGIN      
        1 gacttcgcga gagagttttt ccaggaaacg atagtcgtca taactgagag cagtttgcgc
       61 cacggtgcta ccatcagcgg ccttctgtac cagcgcgccg ttataagtaa tgcagtagtc
      121 gcccggctgt tccatatgca gctctttcag gtagttgtga acgcctgcat aagggcgacc
      181 cgtcgttagc acgacattca cgccacgggc gcgagctgcg gcaatcgcat ttttaacggc
      241 gggtgaaatg gtgtgatcgg gcagcagaag ggtgccatcc atatcgatag caatgagttt
      301 gatagccatg agttccccag gtagattagt tcctgaccca tgctaacgcg attccgctca
      361 aaaatcagta caacacccga gggaaaaggg ggatgcaacg cgggttgcat gctccccatt
      421 tgcttagcgg aagagtttcc ctttcagcag ttccatacct gcggaaagca gatcgttatt
      481 ggcttgtgaa gacacttcac cttgcggtga gagcgcatca ataatcttcg gcaattgttc
      541 tgccagtaaa ctggaagccg tactggtatc cacgccaagt ttttgcccga gatcggacac
      601 cgcatttgtg ccgagcgccg attccagttg ctcgccgcta actgattgat tgctctgctg
      661 attactcaac caggttgaga gaatggcccc taagccgcca ctttgcagtt tttccagcag
      721 cacctgaatg ccgccctgct cctcaaccca acttaaaata gcctgatatt tccccgcatc
      781 gcctttcaga aaggcaccga caacttcatc aaaaagcccc atgataatca cctgtaaagc
      841 gttacgtgtt gacccaaaaa gtatagatct gcgggatgat aattgcggat tgcagaaata
      901 aaaagggcgg agatgatctc cgcccttttt ttatagcttc ttgccggatg cggcgttaac
      961 gtcttacctg gcctacaaaa tcattaaaat tcaatacatt gcaagatttt cgtaggcctg
     1021 ataagcgtag cgcatcaggc atgctcgcat ggttaacgcc attaaatatc aatattcgcc
     1081 gctttcaggg cgttctcttc aataaacgca cgacgcggtt ctacggcgtc gcccatcagc
     1141 gtggtgaaca acaggtcagc ggcaatcgca tccttaacgg taacgcgcag catacgacga
     1201 ctttccgggt ccatggtggt ttcccacagc tgttccgggt tcatctcgcc cagaccttta
     1261 taacgctgga tggagagacc gcgacgagac tctttcacca gccagtccag cgcctgctcg
     1321 aagctggcta ccggctgacg acgctcgcca cgttcgataa atgcatcttc ttccagcaag
     1381 ccacgcagtt tctcacccag cgtgcagata cgacgatatt cgccaccagt gataaattcg
     1441 tgatccagcg gatagtcagt atccacaccg tgggtacgca cgcgaacaat cggctcgaac
     1501 aggttttgtt cggcattggt gtggacatca aacttccact ggctgccgtg ctgttctttg
     1561 tcgttcagtt cgctgaccag cgcgttcacc cagcgggtaa cggtctgctc atcagagagg
     1621 tcggcttccg tcagcgtcgg ctgatagata agctctttca gcattgcttt cggataacga
     1681 cgctccatgc ggttgatcat tttctgcgtc gcgttgtact cagacaccag tttctctaac
     1741 gcttcgccag ccaatgccgg tgcgctggcg ttggtgtgca gcgttgcgcc gtccagcgcg
     1801 atagagatct ggtactgatc catcgcttcg tcgtctttaa tgtactgttc ctgcttgcct
     1861 ttcttcactt tgtacagcgg cggctgagcg atgtagacgt gaccgcgttc aacgatttcc
     1921 ggcatctgac gatagaagaa ggtcaacagc agcgtacgaa tgtgcgagcc gtcgacgtcc
     1981 gcatcggtca tgatgatgat gctgtgataa cgcagtttgt ccgggttgta ctcgtcacga
     2041 ccgataccac agccaagcgc ggtgataagc gtcgccactt cctgagaaga gagcatctta
     2101 tcgaagcgcg ctttctcaac gttgaggatt ttacccttca gcggcagaat cgcctggttc
     2161 ttgcggttac gcccctgctt cgcagagccg cccgcggagt ccccttccac caggtacagt
     2221 tcggaaagcg ccggatcgcg ttcctggcag tctgccagtt tgcccggcag gcccgctaag
     2281 tcgagcgcac ctttacggcg ggtcatttca cgcgcgcgac gcgccgcttc acgggcacgg
     2341 gcagcatcga taattttgcc aaccacgatt ttcgcgtcgg ttgggttttc cagcaggtat
     2401 tctgccagca gttcgttcat ctgctgttca accgccgatt tcacctcaga agaaaccagt
     2461 ttgtctttgg tctgggagga gaatttcggg tccggcactt ttacggaaac gaccgcaatc
     2521 aggccttcac gcgcatcgtc accggtggcg ctgactttgg cttttttgct gtagccttct
     2581 ttgtccatgt aggcgttcag ggtacgggtc atcgccgcac ggaagcctgc caggtgggta
     2641 ccgccgtcac gctgcggaat gttgttggta aagcagtaga tgttttcctg gaagccatcg
     2701 ttccactgca acgccacttc gacgccaata ccgtcttttt cggtggagaa gtagaagata
     2761 ttcggatgga tcggcgtttt gttcttgttc agatattcaa cgaacgcctt gatgccgcct
     2821 tcatagtgga agtggtcttc tttgccgtca cgcttgtcgc gcagacgaat ggaaacgccg
     2881 gagttgagga acgacaactc acgcagacgt ttcgccagaa tttcatattc gaactcggtc
     2941 acattggtga aggtttcgag gctgggccag aaacgcacca tggtgccggt tttttcagtc
     3001 tcgccggtaa ccgccagcgg agcctgcggt acaccgtgtt cgtagatctg acggtgaatt
     3061 ttaccctcgc gctggataac cagctccagt ttttgcgaca gggcgtttac taccgaaaca
     3121 ccaacgccgt gcagaccgcc ggacacttta taggagttat cgtcaaattt accgcctgcg
     3181 tgcagaacgg tcatgatcac ttccgccgcc gatacgccct cttccgggtg aataccggtc
     3241 ggaatgccgc gcccgtcatc ctgtacagag acagagttat cggcgtgaat ggtgacgata
     3301 atttctttac agtgacccgc gagcgcttcg tcgatagcgt tatctaccac ctcgaatacc
     3361 atgtggtgca gaccggtgcc gtcatccgtg tcgccgatat acatacccgg gcgcttacgc
     3421 accgcatcca gccctttcag gactttgata ctggaggagt cataagaatt cgacatcaac
     3481 gtttctcgct catttatact tgggttaatc cgttatttta cccttttcca cggtaaacat
     3541 cttcgaattt tcgtccgaca tgtctataac gtgttcagca ctgatcgcgc tgacaaagac
     3601 ctgtgattgc gtcgctttta agcggctggc aagcagcccg cgacgctcat catcaagctc
     3661 agaggcaaaa tcatctatca ggtagagaca ccgccgcccg ctttcacggg tgaggaactc
     3721 tccttgcgcc agacgtaagg cgcacatcaa cagcttaagc tgcccacgcg ataaggtatc
     3781 ttccaccggc gcaccgtcgg cgcgaatgcg taagtccgct ttatgcgggc catgcgcggt
     3841 gtaggttagc tggcgatcgc gttcaaaatt acgttccagc acctcagcat attctgtctc
     3901 tttctcccag ccgcgctgga aagagaaagt cagagaaaac tcagggagaa attgcttaca
     3961 agtatcggcc atatcagccg cgataccggc gctatactcc gcgcgccagg tgctgatttg
     4021 ctccgccagc gggatcagct ctttatccca cgggcgtagc tgttcgtaac gtgtcacctg
     4081 gcgcagcgcc gcattgcgct gcttgagcaa tcgcttgaga ttgctccagg cggtgaaaaa
     4141 tccgggttcg ttgtgaaagc atgcccagtc gagaaatgct cttctgtatt tggggccgcc
     4201 gttgagtaaa gtaaaccctt ctggcgttat cagctgcatc ggcatcaggt gcgccagctc
     4261 cgcgacttta tgcccgtcag taccgtcgat gcggactttg ctgtcgcctt gtttgtcttt
     4321 ggttaagcca atcgccgtct cgcgctcttc gccctgtaat cgcccatgga gaacaaatgc
     4381 ctcctgctca tggcgaatga cgcgaccaat ctgcaaactg cgaaacgccc gaccatggcc
     4441 gagcgtatag atggcttcca gcacgctggt tttgccactg ccgttggcac ctaccagaaa
     4501 gttaaagccg ggagataagg cgagatccgc ggtttcgata ttgcggaaat cgcggatcaa
     4561 caagcgggtg agggacatta cagtctcatt ggcatgacaa cataagccgc gctctggctg
     4621 gccgcatctt caatctgcac gctggaaacc gaatcggtca gcatcatgcg gacgttttcg
     4681 catttcagcg cgttcagaac atccagcaca tagctgacgt tgaagccgat ttccatctcc
     4741 gcaccgctat aggtaacgtc gaggatctct tccgcttctt cctgttccgg gttgttggca
     4801 gtgattttca gctggttttc gctgacataa agacgtacgc cgcggaattt ctcgttagag
     4861 agaattgccg cacgggcaaa cgcctgcttg agcagatcgc agccagcttc cagatgtttg
     4921 tccgggttct tcggtagaac gcggcggtaa tccgggaagc gaccatccac cagtttggag
     4981 gtgaagataa agtcgccaac gtgggcacga atgttattgc tgccaatctg cacgcgcagc
     5041 ggattgtcgc cgccgtcgag catacgcatc agttcaatca cgcctttacg cggcacgatc
     5101 accgaatggc ttggcaaaga ttgaccaatt ggcattgaac agactgccag acggtgacca
     5161 tcggtcgcta cggtgcgcag ttcttcgcct tcggtttcaa acagcatacc gtttaagtaa
     5221 tagcgaacat cctgatgcgc catcgaaaac tgggtcgctt caatcagacg cttcatcgtc
     5281 gcctgcggca gggtaaattc gacttcgctc tgccagtcat cgaggttcgg gaaatccgcc
     5341 gctggcaggg tagacagcga aaaacggcta cgcccggagc gcaccagcat ccgttcacct
     5401 tccagctgca cggcaatttc cgcgccttca ggcagaccac ggcagatatc aaagaatttg
     5461 cgcgccggaa cggtcgttgc tcctggctcg tgtggctgaa ccagcgcaac acgtgccacc
     5521 atttccatct cgagatcagt accggtcagc gacaacgtac cgtcagcaac ctgtaacagc
     5581 agattaccga gaatcggtag cgtaggacga ccacctaacg gaccgctcac ctgttgtagc
     5641 ggttttaata aatgctcacg ttctacggta aatttcatag gtttacgatg acaatgttct
     5701 gattaaattt gaaaaatctt ctttgatatc gtggctctct tcacgcaact gctcgatctt
     5761 acggcaggca tgaagcaccg tcgtgtggtc acgaccacca aacgcatcgc caatctccgg
     5821 cagactgtgg ttagtcagct ctttcgccag cgccatcgcc atctggcgcg gacgcgccac
     5881 cgagcgggat cgacgcttgg aaaggagatc cgcgactttg atcttgtagt actccgccac
     5941 cgtcttctga atattgtcga tggtgaccag tttttcctgc aatgccagca agtcgcgcag
     6001 cgcctcacgc acgaagtcga tggtgatcgc ccgtccggta aagttggcat tggcaatgac
     6061 gcggttcagc gccccttcca gctcacgtac gttagatcgt agacgcttgg cgataaagaa
     6121 cgccacttcg cccggcaaac gaatgtcgtt ttcgtcggcc tttttcatca ggatcgccac
     6181 acgggtttcc agctctggcg gttcgatcgc cacagtcagt ccccaaccga agcgggattt
     6241 caaacgatcc tcaacgccgt tgatctcttt cggatagcga tccgaggtga gaatgatctg
     6301 ttgattacct tccagcaggg cgttgaaggt atggaaaaac tcttcctgag atcgttcttt
     6361 attagcaaaa aactgaatat cgtcgatcag cagtgcatct acggaacggt agtagcgttt
     6421 aaactcttcg atcgcgttgt tttgcagggc tttaaccatg tcctgaacaa agcgctcgga
     6481 gtgcatataa accactttgg cattcggctt gcgcgccata atgccgttac ccaccgcatg
     6541 cagcaggtga gttttaccca gacccgtgcc gccataaagg aacaacgggt tataggcacc
     6601 gccagggtta tccgccacct ggcgagccgc cgcgcgcgcc agttggttag atttaccttc
     6661 aacgaagtta tcaaacgtgt gtttgacgtt tacgttagaa cgataggtcg gttctgccgg
     6721 ggccgggacg ttatcccaac ctgagcgcgt agaaggcgca gcacgttgcg gctgcgtttg
     6781 cgccacctgt gcaggggccg cgacgttgct cgtcactgcc gcttgtggcg tttgcgtcac
     6841 cggtttggtg ccgacttcaa aacgcagctg tggggcatcc gctccgcaga aactggttag
     6901 cagtccattg atattattaa ggtacttgtc ccgtacccaa tcgaggacaa aacggtttgg
     6961 cgcgtacagg gccagcgtgt tatcgctcag ttccgcctgc aatgggcgta tccacatact
     7021 gaattctgtg gctggtaact catcctgcaa tcgggcaaga cactgctgcc aaagcgaaag
     7081 tgacacggcg gactccactc gaacaaaagt cgataatgac taaggctgaa acattcatga
     7141 ttgttgacgt acgtcgaaaa gaccctgctt gagggtgacg cacgaaccgc tgtctgcggt
     7201 tatatgcccg atcaagatcc tgcaaaacga tcgggaccgc ggatcatagc ctaaactgcg
     7261 caagagatct tctgtttctc acagattttc ccgatttatc cacaggactt tccagaactc
     7321 gctaagtgta aacgatcctg ccgcgaggcg ggcacgattt acgccgcata ttgaaaaatt
     7381 taatgaccat agacaaaaat tggcttaatc gatctaataa agatccagga cgatccttgc
     7441 gctttaccca tcagcccgta taatcctcca cccggcgcgc catgctggtt tccactggtg
     7501 tgaggtcgta cattttccct gcgaaaaggt gcggaaaagc gcggtaaata aggaaagaga
     7561 attgactccg gagtgtacaa ttattacaat ccggcctctt taatcaccca tggcttcggt
     7621 gtccatcgtt tcatttttcg gcggatatcc aataaagcca ttgaatttat tcaagtttag
     7681 gtagaaatcg ccatgaaacg cacttttcaa ccgtctgtac tgaagcgcaa ccgttctcac
     7741 ggcttccgtg ctcgtatggc tactaaaaat ggtcgtcagg ttctggcacg tcgtcgtgct
     7801 aaaggccgcg ctcgtctgac cgtttctaag taataaagct aacccctgag tggttaagct
     7861 cgcatttccc agggagttac gcttgttaac tcccagtcaa ttcacattcg tcttccagca
     7921 gccacaacgg gctggcacgc cgcaaattac cattctcggc cgcctgaatt cgctggggca
     7981 tccccgtatc ggtcttacag tcgccaagaa aaacgttcga cgcgcccatg aacgcaatcg
     8041 gattaaacgt ctgacgcgtg aaagcttccg tctgcgccaa catgaactcc cggctatgga
     8101 tttcgtggtg gtggcgaaaa aaggggttgc cgacctcgat aaccgtgctc tctcggaagc
     8161 gttggaaaaa ttatggcgcc gccactgtcg cctggctcgc gggtcctgat agccctcatt
     8221 cgggtctatc aacgcctgat tagtccgcta ctcgggccgc attgtcgttt cactccaacc
     8281 tgttcaagct acggaattga ggcattgcgc aggtttggag tgataaaagg cagttggttg
     8341 acggtgaaac gcgtattaaa atgccaccct ttacaccctg gtggtgacga tcccgtcccg
     8401 cccggaccat ttgataccag agaacactaa cgatggattc gcaacgcaat cttttagtca
     8461 tcgctttgct gttcgtgtct ttcatgatct ggcaagcctg ggagcaggat aaaaacccgc
     8521 aacctcaggc ccaacagacc acgcagacaa cgaccaccgc agcgggtagc gccgccgacc
     8581 agggcgtacc ggccagtggc caggggaaac tgatctcggt taagaccgac gtgcttgatc
     8641 tgaccatcaa cacccgtggt ggtgatgttg agcaagctct gctgcctgct tacccgaaag
     8701 agctgaactc tacccagccg ttccagctgc tggaaacttc accgcagttt atttatcagg
     8761 cacagagcgg tctgaccggt cgtgatggcc cggataaccc ggctaacggc ccgcgtccgc
     8821 tgtataacgt tgaaaaagac gcttatgtgc tggctgaagg tcaaaacgaa ctgcaggtgc
     8881 cgatgacgta taccgacgcg gcaggcaaca cgtttaccaa aacgtttgtc ctgaaacgtg
     8941 gtgattacgc tgtcaacgtc aactacaacg tgcagaacgc tggcgagaaa ccgctggaaa
     9001 tctccacctt tggtcagttg aagcaatcca tcactctgcc accgcatctc gataccggaa
     9061 gcagcaactt cgcactgcac accttccgcg gcgcggcgta ctccacgcct gacgagaagt
     9121 acgagaaata caagttcgat accattgccg ataacgaaaa cctgaacatc tcttcgaaag
     9181 gtggttgggt ggcaatgctg caacagtatt tcgcgaccgc gtggatcccg cataacgacg
     9241 gtaccaacaa cttctatacc gctaatctgg gtaacggcat cgccgctatc ggctataaat
     9301 ctcagccggt actggttcag cctggtcaga ctggcgcgat gaacagcacc ctgtgggttg
     9361 gcccggaaat ccaggacaaa atggcagctg ttgctccgca cctggatctg accgttgatt
     9421 acggttggtt gtggttcatc tctcagccgc tgttcaaact gctgaaatgg atccatagct
     9481 ttgtgggtaa ctggggcttc tccattatca tcatcacctt tatcgttcgt ggcatcatgt
     9541 acccgctgac caaaacgcag tacacctcca tggcgaagat gcgtatgctg cagccgaaga
     9601 ttcaggcaat gcgtgagcgt ctgggcgatg acaaacagcg tatcagccag gaaatgatgg
     9661 cgctgtacaa agctgagaag gttaacccgc tgggcggctg cttcccgctg ctgatccaga
     9721 tgccaatctt cctggcgttg tactacatgc tgatgggttc cgttgaactg cgtcaggcac
     9781 cgtttgcact gtggatccac gacctgtcgg cacaggaccc gtactacatc ctgccgatcc
     9841 tgatgggcgt aacgatgttc ttcattcaga agatgtcgcc gaccacagtg accgacccga
     9901 tgcagcagaa gatcatgacc tttatgccgg tcatcttcac cgtgttcttc ctgtggttcc
     9961 cgtcaggtct ggtgctgtac tatatcgtca gcaacctggt aaccattatt cagcagcagc
    10021 tgatttaccg tggtctggaa aaacgtggcc tgcatagccg cgagaagaaa aaatcctgat
    10081 tcggtgagtt ttcgctaaaa taagggcggt cagttgaccg cttttttctt ttcgtagggc
    10141 ggataagcac cgcgcatccg ccacacaaag caacaggaac atcatgagcg ataatgacac
    10201 tatcgtagcc caggccacgc ctccgggacg tggcggcgtt ggcatcctgc gcatctccgg
    10261 cttcaaagcc cgtgaagttg ccgaaaccgt gctgggtaaa ctgcctaagc cgcgctacgc
    10321 cgattatctt ccgtttaaag acgccgacgg cagcgtgctc gatcagggga ttgcgctatg
    10381 gttccctggc ccgaactcgt tcaccggcga agatgtgctg gaactgcaag gtcatggcgg
    10441 tccggtgatc ctcgacctgc tgttaaaacg cattctgacc attcccggcc tgcggattgc
    10501 tcgccctggt gagttttccg aacgcgcgtt tcttaacgat aaacttgact tagcccaggc
    10561 cgaggcgatt gccgatctta tcgacgccag ttcggaacag gcggcccgtt cggcacttaa
    10621 ctcgctgcaa ggcgcattct ccgcacgggt taatcatctg gtagaagccc tcacccactt
    10681 gcgcatttac gtcgaagcgg caattgattt ccccgatgaa gagatcgatt tcctctccga
    10741 cggaaaaatt gaagcccagc tcaatgacgt tattgccgat cttgatgcag tgcgtgctga
    10801 agcacgtcag ggtagtttgt tgcgcgaagg gatgaaagtg gtgattgccg gacgtcctaa
    10861 cgccggtaaa tcgagcctgt taaacgcgct ggcggggcgt gaagcggcaa tcgtaaccga
    10921 tatcgccgga actacgcgtg acgtgctgcg tgagcatatc cacattgacg gaatgccgct
    10981 gcatatcatc gataccgccg ggctacgtga agccagtgac gaagtagaac gtattggtat
    11041 cgagcgcgcg tggcaggaaa ttgaacaggc cgaccgcgtg ctgtttatgg tcgatggcac
    11101 cacaacagac gccgtggatc cggcagagat ctggccggaa tttattgccc gtctgccagc
    11161 gaaactgccg atcaccgtgg tgcgcaataa agccgatatc accggcgaaa cgctgggaat
    11221 gagtgaagtg aacggtcacg tgttaattcg tctctcggca aggactggtg aaggcgtgga
    11281 cgtgctgcgt aaccatctca aacagagcat gggctttgac accaacatgg aaggcggctt
    11341 cctggcgcgt cgtcgccacc tacaggcgct ggaacaggca gcggaacatc tacaacaggg
    11401 caaagcgcaa ctgttgggag cctgggcagg tgaactgctg gcggaagagt tgcgtctggc
    11461 acagcagaac ttaagcgaaa tcaccgggga atttacttca gacgacctgc tggggcggat
    11521 tttctccagt ttctgtattg gtaagtaacc gcgcttacga agccgtattc tgactgtcag
    11581 atgcggcttc gcttcattgt tagcactcct gttattcctc aacccttttt ttaaacatta
    11641 aaattcttac gtaatttata atcttaaaaa aagcatttaa tattgctccc cgaacgattg
    11701 tgattcgatt cacatttaaa caattttaga atagacaaaa ttcttgagta ttatactgta
    11761 gcctcgtgtc tttcgaggat aagtgcatta tgaatatctt acatatatgt gtgacctcaa
    11821 aatggttcaa tatcgac
//
LOCUS       DAJTNA010000125        11836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTNA010000125 DAJTNA010000000
VERSION     DAJTNA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11836
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.126"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            132..197
                     /gene="yobI"
                     /locus_tag="OO161_002874"
     CDS             132..197
                     /gene="yobI"
                     /locus_tag="OO161_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5463872.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            336..947
                     /gene="ruvA"
                     /locus_tag="OO161_002875"
     CDS             336..947
                     /gene="ruvA"
                     /locus_tag="OO161_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5463873.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            956..1966
                     /gene="ruvB"
                     /locus_tag="OO161_002876"
     CDS             956..1966
                     /gene="ruvB"
                     /locus_tag="OO161_002876"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5463874.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2016..2801)
                     /gene="znuB"
                     /locus_tag="OO161_002877"
     CDS             complement(2016..2801)
                     /gene="znuB"
                     /locus_tag="OO161_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5463875.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLTGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2798..3553)
                     /gene="znuC"
                     /locus_tag="OO161_002878"
     CDS             complement(2798..3553)
                     /gene="znuC"
                     /locus_tag="OO161_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5463876.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPSTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3632..4564
                     /gene="znuA"
                     /locus_tag="OO161_002879"
     CDS             3632..4564
                     /gene="znuA"
                     /locus_tag="OO161_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5463877.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPGMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4580..5902
                     /gene="mepM"
                     /locus_tag="OO161_002880"
     CDS             4580..5902
                     /gene="mepM"
                     /locus_tag="OO161_002880"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5463878.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6022..6993
                     /gene="lpxM"
                     /locus_tag="OO161_002881"
                     /gene_synonym="msbB"
     CDS             6022..6993
                     /gene="lpxM"
                     /locus_tag="OO161_002881"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5463879.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRCNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7082..10158)
                     /locus_tag="OO161_002882"
                     /pseudo
     CDS             complement(7082..10158)
                     /locus_tag="OO161_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(10196..>11836)
                     /locus_tag="OO161_002883"
     CDS             complement(10196..>11836)
                     /locus_tag="OO161_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCR5463880.1"
                     /translation="SETQSNWKDGYCRSAYLLDNKEWQEKASDLCLELIDTQDDDGYI
                     GIYDSALKFNCKAENGEFWAQATALRFVLGCYESTRNEGLLTSLTQAAQCIMAGYPKE
                     TSHPFVVEHGFAGHSHGLTITDVFYRLYEITNNIEYLSYCLWLYEDYSAGCVSEQDLQ
                     YKNVINPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALDIFLSQLKYYLTPSGAPIG
                     DEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGLADDAEWLFYNASFGMQHP
                     IHHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEAAVCCVPNTARTIPAFVEN
                     MFQENNNGFTALFYGPCEFYGTYNNVAINITQLTEYPHDLSVKCLIEPESSVRFALSF
                     RYPDWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDIKFIADIQFNTDLCGDTYI
                     SRGPVLYAIELESDILIKKTLLNWKYFDSGYIPCSRADESIQFSYTDMESFSYSTSPE
                     GKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
BASE COUNT         3323 a         2628 c         2700 g         3185 t
ORIGIN      
        1 cacctataca tgtaaagacg atatctccaa tttcatattc agcagggtaa ttaatattca
       61 cgttgtcatt ccattgaaat agatacgaat atacataata gcataaaaaa attatttttt
      121 caggaggcgc aatgtatatt tttattacgc atttcttcac tgaatacgta atatttaaat
      181 atttgcttcc aatataacct gtagaataaa tcatactgcg ccatttttca gttcatcgag
      241 acacctcgca agttttcttc atccttcgct ggatatctat ccagcatttt tttatcatac
      301 agcattatct ttgattcatt acgcaggagc gtcatgtgat aggcagactc agaggcatca
      361 tcattgaaaa acaacccccg ctggtgttaa ttgaagtggg cggcgtaggc tatgaagtgc
      421 atatgccgat gacctgtttt tatgaactcc ctgaagcggg tcaggaagcg atcgttttca
      481 cccactttgt ggtgcgtgaa gacgcgcaac tgctgtacgg ttttaacaat aaacaagagc
      541 gcacattgtt caaagagttg atcaaaacca acggcgtcgg cccgaagttg gcgctggcga
      601 tcctctccgg aatgtcagcg cagcagttcg ttaatgccgt tgaacgtgaa gaagtggggg
      661 cactggtgaa actgccgggt attggcaaaa aaaccgccga acgcttgatt gttgaaatga
      721 aagaccgatt taaaggtttg catggcgatc tgtttacccc ggccgccgac ctggtactca
      781 catcacctgc cagcccggcg accgacgatg ctgaacagga agcggttgcc gcgctggtgg
      841 cgctgggcta taaaccacaa gaagcaagcc gcatggtgag caaaatcgct cgccctgacg
      901 ccagcagtga aacattaatt cgcgaagccc tacgcgccgc gttatgaggt aaaggatgat
      961 tgaagcagac cgtctgattt ctgccggtac cactttgccg gaagatgtag cagatcgcgc
     1021 cattcgccct aaattactgg aagagtatgt tggtcagccg caggttcgtt cacagatgga
     1081 gattttcatc aaagcagcga aactgcgcgg cgatgccctc gatcatttgt tgatttttgg
     1141 tcctccgggg ttgggtaaaa ctacgcttgc caacattgtc gccaatgaaa tgggcgttaa
     1201 tttacgcacg acttctggtc cggtgctgga aaaggcgggc gatctggcag cgatgctcac
     1261 taaccttgaa ccgcatgacg tgctgtttat tgatgagatc caccgtttat cgccagttgt
     1321 tgaagaagtg ctgtacccgg caatggaaga ctaccaactg gatattatga ttggtgaagg
     1381 tccggcggca cgctccatta aaattgattt gccgccgttt accctgattg gcgcaaccac
     1441 gcgcgcaggt tcgctgacat caccgttgcg cgaccgtttt ggtattgtgc aacgtctgga
     1501 gttttatcag gtgccggatc tgcaatatat cgttagtcgc agtgcacgct ttatggggct
     1561 tgagatgagt gatgacggcg cgctggaagt tgctcgtcgc gctcgcggta cgccacgtat
     1621 tgccaaccgt ctgctgcgtc gagtgcgtga tttcgccgaa gtgaagcacg atggcaccat
     1681 ctcggcagat atcgctgctc aggcgctgga catgttgaat gtcgatgctg aaggtttcga
     1741 ttatatggac cgcaaattgt tgctggcggt aatcgataag ttctttggtg ggccggtagg
     1801 tctggataac ctggcggcgg ctattggcga agaacgtgaa accattgagg atgtgctgga
     1861 accttatttg attcagcaag gctttttgca gcgtacaccg cgtgggcgta tggcgacgac
     1921 gcgggcgtgg aatcactttg gcataacgcc gccagaaatg ccgtaagtcg gattggtcgc
     1981 tcgcgccgca tccgacaaat gtgttcagcg atagattagc tggcctgctt tttcatcata
     2041 ctgataataa ataacagtgc tgcacatagc accaccgacg gacccgccgg cgtatcgtaa
     2101 aatgcggaaa aggttaaacc gcccgttacg gcaaccatcc ccaccaacac agcgacacct
     2161 gccatctgtt ccggcgtgcg ggcaaagcga cgtgcagtag cagcaggaat aatcagcagt
     2221 gaagtaataa tcaacgcgcc aacaaatttc atcgctacac caatcgtcaa tgccgtcacc
     2281 agcatcaaca acaatttcac gcgctgtaat ttcacaccat caacaaacgc cagatccggg
     2341 ctaatcgtca tcgacagcaa attgcgccat tgccagaaca aaatagccac cacgatgacc
     2401 acgccaatcg caatagcgat gagatcttct ggcgtcactg ccagcaaatc accgaacagg
     2461 taagccatca aatcaacacg aatgttagac atcagactaa cgaccaccag gcccagcgac
     2521 agggcactgt gcgccataat ccctaataac gtgtcaatcg ccagctgtgg acgcttctcc
     2581 agccatacca gaccgcccgt caggagcagc gtaacggcaa ttaccgcata gaatggattc
     2641 acgtccagca acaaaccaaa cgcgacgcca agtaatgagg catgagccag cgtatcaccg
     2701 aaataagaca tacgacgcca gactacaaac gaacccagcg gacccgcggc acaggcgagc
     2761 atgatcccgg ctaaccaacc gggaaataat aattcaatca tgagcgatca tttccccgac
     2821 gcaaaacaat tcgtccctgt aaatcgtgac gatgattatg atgatggcga tagataccca
     2881 gttgttcagc accacgagga ccaaacatag aaataaactc cggatgtagg gaaacaactt
     2941 ccggtgtgcc ggaacaacaa atgtggtgat tcagacacag cacttcatcg gtttttgcca
     3001 ttaccagatg cagatcgtga gagaccatta aaacgccaca atccagttcg cgacgcaact
     3061 ggtcaataag gtcatataac gccacctggc catttacatc cacgccttga gtgggttcat
     3121 ccagcactaa taattgcggt cgatttaaca atgctcgcgc taacagtaca cgctgtgttt
     3181 cgccaccaga gagcttttgc atcggtgcgt taatcagatg cccggcctgg acacgtttca
     3241 gtgcaggcaa aatatcttct ttatgtgtgc tagggcgtaa gcgtaaaaaa cggtttacgg
     3301 tcagtggcaa cgtggtgtcg agatacagct tctgcggtac atagccgatg cgtagttttc
     3361 cgttgcgctt gataacccct tcatcaggtg ttaccagccc gagcactacc cgtaccagtg
     3421 tcgacttacc tgcgccattt ggcccaagta aagtcaaaat ttttccaggt ttaagttcca
     3481 gcgacacatc agagaggacg cggcgttggc caaaagaaac cgagacattt tccagggaaa
     3541 ccagacttgt catgttaatt ttagtcttgc agtagtcatg aaatgttata atatcacact
     3601 tctcatattc attacgatga ttggtcgcat tatgttacat aaaaaaacgc ttcttttcgc
     3661 agcattatcc gccgctctct ggggaggtgc aacacaggcc gcagatgccg ccgttgtcgc
     3721 ttcgcttaaa cccgttgggt tcatcgcttc tgccattgct gatggtgtaa cagaaacgga
     3781 ggttttactg cctgacggcg cttcagaaca tgattattca ctgcgtccat cggatgtaaa
     3841 acgcttacag aacgcggact tagtcgtttg ggttggcccg gggatggaag cgtttatgca
     3901 aaaaccggta agcaaattac taggagcgaa gcaggtaacg attgcgcagc ttgaagatgt
     3961 gaaactgcta ctgatgaaaa gtattcacgg cgatgatgat gatcacgacc acgcggaaaa
     4021 aagtgaagaa gatcaccatc acggcgattt caacatgcat ctttggcttt ccccagagat
     4081 agcgcgggct acagcggttg caatccatgg aaaattagtg gaacttatgc cgcaaagtcg
     4141 agccaaactt gacgccaacc tgaaggattt tgaggcacaa ttagcctcaa ccgaaacgca
     4201 ggttggtaac gagctcgcgc cgctcaaggg gaaaggttat ttcgtttttc acgatgctta
     4261 cggctatttc gaaaaacagt tcggactgac accgcttggt cattttaccg ttaaccctga
     4321 gattcaacct ggcgcgcagc gtttacatga aataagaaca cagttggttg agcaaaaagc
     4381 aacctgcgtt tttgctgagc cacagttcag gccagcggtc gttgagagcg tcgcacgagg
     4441 gacatccgtt cgtatgggaa cgttggatcc cttggggacg aatatcaaac tgggtaaaac
     4501 aagctattca gaattcctga gtcaattagc caaccagtat gcgagctgcc tgaaaggaga
     4561 ttaatgagga agtgattacg tgcaacagat agcccgctct gtcgccctgg cgtttaataa
     4621 tttaccgcga ccacaccgcg ttatgttggg gtcgctcacc gttcttactc tggccgtcgc
     4681 tgtctggcgg ccctatgttt atcaccgcga tgccacgcca attgtcaaaa ccattgagct
     4741 ggaacagaac gaaattcgtt cgctcttacc tgaagccagt gagccgattg atcaagctgc
     4801 acaagaagat gaagccattc cccaggacga actggatgac aaaatcgccg gtgaagcggg
     4861 cgtgcatgaa tatgttgttt ccactggcga tacgctaagc agcattctca atcagtatgg
     4921 tattgatatg ggtgatatca cccaactggc tgcggccgac aaagaattgc gtaacctgaa
     4981 aatcggtcaa caactctcct ggacattaac cgcggacggc gaactgcagc gcctcacctg
     5041 ggaagtgtct cgtcgtgaaa cccgaaccta tgaccgtact gccgctaacg gttttaaaat
     5101 gaccagcgaa atgcagcaag gagagtgggt taacaatctg ctgaaaggta ccgtcggagg
     5161 gagctttgtt gccagcgcca gaaacgccgg tttaaccagc gccgaagtga gcgcagtgat
     5221 taaagccatg cagtggcaaa tggatttccg caaactgaaa aaaggcgatg aatttgcggt
     5281 gttaatgtct cgagagatgc ttgatggtaa acgtgagcaa agccagctgc tgggcgtacg
     5341 tttgcgttca gaaggtaaag attattacgc aatccgcgct gaggatggca aattctacga
     5401 ccgtaacggt actggtctgg cgaaaggatt cttgcgattc ccgacggcga aacagttccg
     5461 tatctcgtct aactttaacc cgcgtcgtac taatccggtg accggtcgcg ttgcaccaca
     5521 cagaggtgtt gatttcgcca tgccgcaggg tacgccagtg ctttcagtgg gtgacggtga
     5581 agtggtggtt gccaaacgca gtggcgcagc aggttattat gtggctattc gtcatggtcg
     5641 cagctacacc acgcgttata tgcacttgcg caagattctg gtgaaaccgg gacagaaggt
     5701 gaaacgtggc gaccgtatcg cgctttccgg taataccgga cgttcaaccg ggccgcatct
     5761 gcactatgaa gtatggataa accagcaggc cgtaaacccg ctgacggcaa aactgccgcg
     5821 taccgaaggg ctgaccggct ccgatcgtcg cgaattcctg gcgcaggcca aagagattgt
     5881 gccgcagcta cggtttgatt aattaacatc cattcgcagc cggtacgcag tcagtaccgg
     5941 ctttttttat ttggtgcggg gcaagttgcg ccgctacact atcaccagat tgatttttgc
     6001 cttatccgaa actggaaaag catggaaacg aaaaaaaata atagcgaata cattcctgag
     6061 tttgataaat cctttcgcca cccgcgctac tggggagcat ggctgggcgt agcagcgatg
     6121 gtgggtatcg ctttaacgcc gccaaagttc cgtgatccca ttctggcacg gctgggacgt
     6181 tttgccggac gactgggaaa aagctcacgc cgtcgtgcgt taatcaatct gtcgctctgc
     6241 tttccagaac gtagtgaagc tgaacgcgaa gcgattgttg atgagatgtt tgccaccgcg
     6301 ccgcaagcga tggcaatgat ggctgagttg gcaatacgcg gaccggagaa aattcagccg
     6361 cgcgttgact ggcaagggct gcagatcatc gaagagatga ggtgtaataa cgagaaagtt
     6421 atctttctgg tgccgcacgg ttgggccgtc gatattcctg ccatgctgat ggcctcgcaa
     6481 gggcagaaaa tggcagcgat gttccataat cagggcaacc cggtttttga ttatgtctgg
     6541 aacacggtgc gtcgtcgctt tggcggtcgt ctgcatgcga gaaatgacgg tattaaacca
     6601 ttcatccagt cggtacgtca ggggtactgg ggatattatt tacccgatca ggatcatggc
     6661 ccagagcaca gcgaatttgt ggatttcttt gccacctata aagcgacgtt gcccgcgatt
     6721 ggtcgtttga tgaaagtgtg ccgtgcgcgc gttgtaccgc tgtttccgat ttatgatggc
     6781 aagacgcatc gtctgacgat tcaggtgcgc ccaccgatgg atgatctgtt agaggcggat
     6841 gatcatacga ttgcgcggcg gatgaatgaa gaagtcgaga tttttgttgg tccgcgacca
     6901 gaacaataca cctggatact aaaattgctg aaaactcgca aaccgggcga aatccaaccg
     6961 tataagcgta aagatcttta tcctatcaaa taaaaaaaag cctctcaaat tattgagagg
     7021 ctgtactcga taaaaaatct caatgagtgt tcccactatt ttgagttttt aggttagtac
     7081 gctattgagt taatcgtagc gtgataattt caaaaggttt gaactcaaga ttgcaatatt
     7141 gattgcaata aaaactgtca tctaacttat tttcttgaag atcgacatat tgaacgtagt
     7201 gataattaca tacccacgaa agcatggctg aaatttctaa tccatgactc tcatataatc
     7261 ttacgattat accgtcgccg tcttcagcta attttattgt ttcgatgata acgctgttag
     7321 tatcgatagc aaaaaatgat agtgaagggg gtaattcacc cgggtgcggt ttgacaggga
     7381 cgacctgtaa gggatgattc attatatagg cttgattaat aacaccaccc acacggtaat
     7441 ctccatgatg aggaaataag gagaatatga atttttgttt accaatatct gcaaaattat
     7501 taatcaggaa attatcataa ttgtctgtct taacaacttc cccaggttta tgctgcgaac
     7561 gcagcaaagt taattcaagc gtctggtctt tgacacgata gccatatttg ttttttgcta
     7621 ttaaagctat gccagtttct cgatcagaaa ggtcgaccca gtgatgtgct ggtatttcat
     7681 ctttggcaaa agcgaacgaa tcatcgctat gcgttggtct gtcaattaca ccgaactgaa
     7741 tttgacattg tgcaagagat gtttgtactg acaccgggga tttgactttg agagaaacat
     7801 taggttcgtg ccaatcaata aaagtttgga catcaacgcg tggactgtcc tgtgtcaggg
     7861 taaatgcgaa gcgaagataa gaggaattgt agtgataaat cagcgtcatc gtagttttag
     7921 gcccgctgat tacagttgtc attttttcag cgagtaaaac atatgattcc ccatcacgat
     7981 aatttgaagc aaaatcccag gccgcgaaaa aacctgcatc ttcgtggtat gcacggatct
     8041 cactgtgcat attttcagta atgtattctt taccatatct tttatcatat agcgaaatta
     8101 aatcaccgtt ttctgaaaaa agaagtttaa tgtaattatt ttcaatactt cgagattctg
     8161 ccttcagacc atttacgatc ttattctttg gatcaatcac agcataacca taagagttta
     8221 ctcgtgcttt gagccagttg ttgtttatat tgatccattc gttacgagca aaacaggtgg
     8281 tattaaataa aatgtgcgga tcttcataag aagaagtatc aattttactt aataaagtag
     8341 actgcgtatc agaaataatt ttctcggttt ttgcttctag tttaatatat tccttttcag
     8401 tttcaagata aactcttgaa atacatgatc cgggaaggat gtcatggaat tgcaaggtca
     8461 gcatctcttt ccatatttca tcaaactcac tgcgaagaat tgatgtcata ttattagtaa
     8521 tagttgtaaa aaattcagca tcatgtaatt tattttccat atttcgatta tgagctttag
     8581 ttgctgactc tgatgtaaag cacccttgat gagcctcgaa ataaagttca cctgaaataa
     8641 tgggcaagct ttcgcgaaag gcgtcggcgt aagtgaagaa cttatcaaca cggctgaaat
     8701 caacatgagg taagccatca atattgcgta tacgtgttaa tcgttctata tgttcctcac
     8761 cggggccagc gccgccatca ccgacaccat acaccataag cgcataggga cagatctctt
     8821 tttcattata gttttgttca atatattcta aagagcgagc acgtgctcgg ctatcatacg
     8881 tgtcttcagg aaagttatgc atcagaattt ctgagccatc caggccctgc cagcggaaaa
     8941 ggtaattatt aaatttatta atcttgtttt gtgagagctt ttgcgatgca atatagttaa
     9001 tgcctgattt ttttgagaat ttgtggaagc gttgcgggga atccaaagac atccggttgc
     9061 cagacataat ttatcttttt tgaaaaattc tgcagtgtga atcgtgtccc atgcatgatt
     9121 tgccttatca gcgattcacc acttaccaga tttaaatcgc attcgaccca gaagcaacct
     9181 tgtaactcaa agcggcctgc ggcgatttgt ttttttaact tttcgaagaa atagggataa
     9241 tcttttttta tccagtgaaa aagttggtac tgactagcgc caaaaatgta atcaggatat
     9301 ttttcaatgt ttgcaagggc ggtggcaaat gttcttattg cttttctgcg tccttctctt
     9361 aaaggctaca tccacgctat atctaaatga gcatgaccag tagccataat cctgaactct
     9421 gaaggattat tgtttttatt gattatggat tggattgcaa tttgacattt aattatttct
     9481 tccattgcta aaggtaatta acgtattatc aatatattcg agagtactta tgatttcgtc
     9541 agtacgctca ctttttttgg caataaattg ttctggggtg atacctttag gatgtatgga
     9601 ttcaaacttc tgaccatcat taatccagtc gaaaagcacc tcaatatcat aataaaggga
     9661 tttcagtagt tgattacatg tcgcgatatg aacatcagta ataatccctc cattagtaac
     9721 atagccaaat aaatcgttgc aggcagcatc aatccaaaac tcaacctcgt cattttcaca
     9781 aacgagagaa agatcaacca cccatttacc cggtttatct ggcggtacgc cataagatga
     9841 tgttttattc gtgatgccgc agatgggtga accattctta tccacaacaa gaccttcgcc
     9901 accgatatca acgagagcga cgaatttgtg tctaaattta tcggctggac gtttaccata
     9961 aactcgcatc caggcacagt caaacagttg accccagtct gagccaacgg taagtttctg
    10021 gtatgcgccg ctacggcgat aaacaaaaga ctgcgattct tttgttatcc atgcttcaat
    10081 cgacatttca gagacaacag tataaactct ctgacctatt atattcgaca gtttaataat
    10141 attatcatta aggtccatag aaatcccttt aattaaatat aatactcgaa aaatgttaac
    10201 tctctatatt ggagaaagtt actttgcgaa gaatagtttg accaaatggt ataagttttc
    10261 tgactatttt agtcttgttt aaataaaaac acccttcaat gtattgcttg ccctctggtg
    10321 aggttgaata tgaaaaagat tccatatcgg tataagagaa ctggatagat tcatcagctc
    10381 tactacaggg tatgtatcct gagtcaaaat atttccagtt cagcagagtt tttttgatta
    10441 aaatatcgct ctcaagctca attgcatata aaacgggacc tctggaaata taagtgtcac
    10501 cacataagtc agtattaaac tgtatatcag ctataaattt aatgtcaatc acatcatgat
    10561 gttgccatgt tctatctaaa ataataagtg agttttgagt atcattttta gtaaaagtaa
    10621 caccattaat aatcatcatt tttgcccagt cggggtaacg gaaactgaga gcaaaacgaa
    10681 cagatgactc aggctcaatg agacatttta cgcttaaatc atgagggtat tcagttaatt
    10741 gtgtaatatt aattgcaaca ttattatatg tgccataaaa ctcacacgga ccataaaata
    10801 atgcagtaaa accattgtta ttttcttgaa acatattttc gacaaaagcc ggtatggttc
    10861 ttgctgtatt cggtacgcag cagacagcag cctcctcatg agtaggggag tatttatatc
    10921 tggtattaaa ctgattactg tccggatgtt ttctttcatt tgcttcataa caattatcag
    10981 tcttacagta cattatcgcg tgatgtatgg gatgctgcat gccaaaactg gcattataga
    11041 atagccactc agcatcatcg gccagaccaa tattagtggt cagttttatt aacataacat
    11101 aagtgctaaa tagttcatga acagaacaat attcatagcc tgtataagtg gcattagctg
    11161 tgcgtcctgt aatccactca tcaccaatgg gagcgccaga tggtgtaaga taatatttca
    11221 actgggataa aaaaatatct aatgcagttt gatactgcgg ccgttggctc gctgcgatgg
    11281 ctaatgccct aatatgctca tatgtatgta cgccatgacc tttaagaagg taaccagggt
    11341 ttatgacatt tttatattgc agatcttgct ccgacacgca tcctgcggaa taatcttcat
    11401 acagccacaa gcaatatgag agatattcaa tgttattggt tatttcataa aggcggtaga
    11461 aaacatctgt tattgttaat ccgtggctat gtccagcaaa accatgttcc acaacgaaag
    11521 gatgtgacgt ttctttaggg tagccagcca taatacattg tgcggcttgg gtaagagaag
    11581 ttaataaacc ctcattacgg gtggactcat aacatccaag cacaaaacgc aaggcagtag
    11641 cttgtgccca gaactctccg ttttctgctt tacagttaaa ttttaatgct gaatcataaa
    11701 ttccaatgta gccatcatca tcttgcgtat cgataagttc caaacataaa tcgctcgctt
    11761 tttcctgcca ttctttatta tctaacaagt aggcactgcg gcaatatcca tccttccaat
    11821 tcgattgagt ttcact
//
LOCUS       DAJTNA010000126        11519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTNA010000126 DAJTNA010000000
VERSION     DAJTNA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.127"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..307
                     /locus_tag="OO161_002884"
     CDS             <1..307
                     /locus_tag="OO161_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5463881.1"
                     /translation="VNAIERDKALAWVEKNIKVPLTEPQKSGIASFCPYNIGPGKCFS
                     STFYRKLNAGDRKGACAEIRRWIYDGGRDCRNRSNNCYGQVSRRDQESALACWGIDR"
     gene            528..641
                     /locus_tag="OO161_002885"
     CDS             528..641
                     /locus_tag="OO161_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463882.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            643..1110
                     /locus_tag="OO161_002886"
     CDS             643..1110
                     /locus_tag="OO161_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5463883.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRVHAIIYKEQRDKKASEL
                     ELANATITDMQIRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQDYIRTQCLN"
     gene            1449..1775
                     /locus_tag="OO161_002887"
     CDS             1449..1775
                     /locus_tag="OO161_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCR5463884.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(1907..2107)
                     /locus_tag="OO161_002888"
     CDS             complement(1907..2107)
                     /locus_tag="OO161_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR5463885.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            2149..2514
                     /locus_tag="OO161_002889"
     CDS             2149..2514
                     /locus_tag="OO161_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5463886.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCRLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAASDMPD
                     PTLPEVSGGSGREDDIIGL"
     gene            2805..3365
                     /locus_tag="OO161_002890"
     CDS             2805..3365
                     /locus_tag="OO161_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5463887.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPPAVMS
                     HGIEYMEDGLPDPVKAMGQILVENLSIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPRLVVNNDNEGNG"
     gene            3362..5023
                     /locus_tag="OO161_002891"
     CDS             3362..5023
                     /locus_tag="OO161_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5463888.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VIPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCINLDDILRRVDVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFHDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASRRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            5087..7023
                     /locus_tag="OO161_002892"
                     /pseudo
     CDS             5087..7023
                     /locus_tag="OO161_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            7068..7289
                     /locus_tag="OO161_002893"
     CDS             7068..7289
                     /locus_tag="OO161_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463889.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYSEPEKNRNQDVT"
     gene            7235..9657
                     /locus_tag="OO161_002894"
                     /pseudo
     CDS             7235..9657
                     /locus_tag="OO161_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            9654..9980
                     /locus_tag="OO161_002895"
     CDS             9654..9980
                     /locus_tag="OO161_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5463890.1"
                     /translation="MTALLTLEEVKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            9990..10340
                     /locus_tag="OO161_002896"
     CDS             9990..10340
                     /locus_tag="OO161_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5463891.1"
                     /translation="MAISVGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQFGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            10337..10783
                     /locus_tag="OO161_002897"
     CDS             10337..10783
                     /locus_tag="OO161_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCR5463892.1"
                     /translation="MIEILLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVLRTPVRRGKLRRNVVILSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            10780..11124
                     /locus_tag="OO161_002898"
     CDS             10780..11124
                     /locus_tag="OO161_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCR5463893.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVAPLLDGRPLVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            11189..>11519
                     /locus_tag="OO161_002899"
     CDS             11189..>11519
                     /locus_tag="OO161_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463894.1"
                     /translation="MSALYERSQLTQVMISSALATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFK"
BASE COUNT         2594 a         2793 c         3629 g         2503 t
ORIGIN      
        1 ggttaacgcc attgaacgtg ataaggcgct ggcatgggtg gagaaaaaca tcaaagtgcc
       61 gctgaccgaa ccccagaaat cggggatcgc gtcattctgt ccgtacaaca ttggccccgg
      121 taagtgtttc agctcaacgt tttaccggaa actgaacgcc ggagaccgga aaggtgcatg
      181 tgctgaaatt cgtcgctgga tatatgacgg cggcagggac tgtcggaacc gctcaaataa
      241 ctgttacggg caggtatcgc ggcgtgacca ggagagcgcg ctggcgtgct ggggtatcga
      301 caggtagcag aatattttgc tgaaaaatga cgcttgctca cgcgggcgga taacacgaag
      361 tcctgtgaac tggcaaaacc taagtgaata aaagtaaaaa ccccgtttgt tggcagcaag
      421 cggggttttg tgttttctga ccttgagtaa ggcaagggag aaattatggt gagggaggtg
      481 ctttccctgt gaggaagtat aaaagattct ttctgaggtt gtccattatg aaaggcattg
      541 aagtggagac gccagccagt ctggatttaa caagagcggc agcttttgcc attcgtattg
      601 tggcaattgc tgttctggtc tgggcaatcc gttggtggtg atatgaaccg tgttctgtgc
      661 gtggtcatca ttgccctgct ggtggcctgt ggtgcgctta gtctggggct gaatcattac
      721 cgtgttcacg ccatcatcta caaagagcag cgcgataaaa aagccagtga gctggagctg
      781 gcgaacgcga caattactga tatgcagata cgccagcgtg atgtcgctgc acttgatgcc
      841 agatactcga gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt
      901 gccgctggtc gtaagcgcct gcggatcaac gccacctgct ccggtaccgt gcgtgaagcc
      961 accggcacct ccggcgtgga taatgcaacc ggcccccgac tggcagacac cgctgaacgg
     1021 gattatttca tcctcagaga acggttgatg acaatgcaga agcagctgga aggggcacag
     1081 gactatatcc gcactcagtg cctgaactaa gttttgctga tgcgccgtat cgtcgctgta
     1141 ttccctcatt aacagagacc gcagcccgac agggagactc ctctgcgcga gtgtgcgggg
     1201 ataatcaaaa acgatacaca ccggggttta ccgcgttaac ggagcgcggc gttgtcccct
     1261 catagtcgcc agtccggtgc gatggtggaa gaaacaggac gatgtgttac ctcgcaagcc
     1321 ctgttatgtc atgtgtctga tttgtgattt aagtcggata attgtcgttg ccattaagca
     1381 gaggattgat gaccgacagg gcggcattgt tagaataaga cttattttta tctgtgcggg
     1441 gaatgaaaat gaaaagaaat cttccgttaa ttattttgtt gtcttctctg gttatgggct
     1501 gtacgcaaca taaaacagat atgccccgac agttggttaa ggcattacca caatatccgg
     1561 cctatgcagc ggcaaattat ataaagggac gggttgatgt gaggtttgat attggtgctg
     1621 atggtactgt cacccgaatt gagtttattc gttcagagcc gcaccatctg tttgatgagc
     1681 aggttgtaaa ggcgatggca aaatggcgat ttgagaagga caggccgtgt aaaggcgtga
     1741 agaaaacgtt tatctttagt ccttccgcac cctgattatt tcatcagaaa ttaattatca
     1801 ctctgttgtt attctgtaca tcccggcagg gtaagtcttg ttccgtcgga tatgaagatg
     1861 aaatattgtt ggaggacagt gggtacctgc tcctgtaacc gaacgttcat ttctcgttat
     1921 ttgtcatgct ggccgggcgc agatgcgttg catctgttgc cagccttctc ctgcaggctt
     1981 caataaccca cgctgaaaag ttaccggacc ctttatgctc aagggcgatg ttgatctgtt
     2041 caatcatgtg attggggaaa cggatattgc gggttgtggt tctgcgggta cggtttttcg
     2101 atgacatatt tatttccttt actgattgcc atatgacggg gattttacat ggctgagctt
     2161 cgtacactcc agagcagaat caaaacactg aatacccgac gggtgaatat tctgaagggt
     2221 gaacagcgtc gtgtcagtgg cagtgcacgt gtttccctca agcgtcatat ctggctcagg
     2281 gacgccgggc agtgccgtct ctgtggtcgt gtggttgacc tctgtgacag tgaactcgat
     2341 caccgaattg cacttcagtt cggtggtggt aatgaggaga cgaatctctg gacgctctgt
     2401 accgaatgcc atcgacaaaa gtctgctcgt gaagcggcga gtgatatgcc ggacccgacg
     2461 ctgccggagg tgtccggagg tagtggcaga gaggacgaca tcatcggact gtaacccgac
     2521 cccggggggt atcatccggc gtaaaaaacg atcgctttgg acaccgcgcc ccctctcacg
     2581 cagagaaaaa attcccgttt caggccagtt aacatgttaa ctggctgtcc gggcattttt
     2641 gcggttttta tctttattat tcagtttgtt gtgcgaaaaa aaatgttaac tggctttttc
     2701 agaaaatgtt aaccaggcag cagttaacat ttgcggcatg agacgccggg aaaaatgggc
     2761 tgaaccatac ccggctgagt gcgttctgga cccgggagga ggctgtgctg acaacgcaaa
     2821 aacgaaaatt tgcgctggcg ctcatgtccg ggaaaaacaa aacagcgtca gccattgccg
     2881 ccggttattc ggcgaagacc gccagggtta aaggctcgca gctggcaaaa gatccggagg
     2941 tgctcgcgtt tatagcccgt aaacagtgcg aaacggtgga ggtggatgag gttcctgttt
     3001 accggcagaa aaaatcagag caggaggata aaccccgtcg ccgtgaggcg gctgcaatac
     3061 cacagccgga cgaaaacaat ccggagatgc caccgcccgc ggtgatgtct catggtattg
     3121 aatatatgga ggatggtctt cccgatccgg tgaaagctat ggggcagatc ctggtggaaa
     3181 acctcagcat tgatccgaaa ctggcactgg atgcggcctg gcgactggcg cagttcacac
     3241 accataaaaa aggcgatgcc ggtaaaaaat cggcaaaagg tgatgccgcg aaaaaagcgg
     3301 ctaaccgttt tgcggtacca ccaccacgac tggtggtgaa taacgataat gagggcaacg
     3361 gatgatacct gtatggagca cggcctgccc ggactgggca gagcgcctga aaaaggggct
     3421 gtcgattatt ccggctccga tttatccgga ccaggccgca catgccctgg cgatttttaa
     3481 acaactgcgg attgtggatg caccgggtag cccgacgttc ggggagtcct gtgcacagtg
     3541 ggtgtttgac ctggtggcgg ctctgtttgg ctcctacgat gcgcagaccg gtgtccgcca
     3601 tatcaaggaa gtgttcattc tgatacccaa aaaaaacagc aagtccacac tggccgccgg
     3661 gatcatgatg acggcgctgt tactgaactg gcggcaggcg gcgggttaca cgattctggc
     3721 cccgactgtg gaggtggcag ccaacgcctt caatcctgcc agggatatgg tacgacggga
     3781 cgatgatctg gatgacctct gtcaggtgca gacacatatc cggaccatca cccaccgggt
     3841 gacggacacc accctgaagg tggtggcagc cgatccgaat acggtgtccg gtatcaagtc
     3901 cgtggggacg ctgattgatg agttgtggct gtttggcaag cagtacaagg cggaggacat
     3961 gttacgtgaa gccatcggcg ggcttgcctc ccgcccggaa gggtttgtgg tgtatacgac
     4021 cacccagtcg aatgaaccgc ccgccggggt gttcagacaa aaactgcagt acgcccggga
     4081 tgtgcgcgac ggcaaaatta atgatccgca ctttctgccg gtgatatttg aacaccctcc
     4141 tgaaatggtg gaaagcgggg ctcacctgct gatggaaaac ctcgccatgg tcattccgaa
     4201 tctcggctat tcagtggatg aggcctttct gtaccgggag taccgtaaag cccgggaagc
     4261 cggtgaagag acattccggg ggttcatgtc aaaacacgcc aatgtggaaa ttggtcttgc
     4321 cctgcgctct gaccgctggg cgggggctga tttctgggaa gagcagggcc gttgtatcaa
     4381 cctggacgat atcctgcgtc gtgttgatgt ggtgacggtg gggattgacg gcggagggct
     4441 ggatgatctg ctggggatgt atgtgattgg gcgtgaccgg gagacccgcg aatggctggg
     4501 ctggggccat gcctgggcgc atgaaaccgc ggtggtccga cggaagagcg aggcgtcccg
     4561 gtttcatgat cttgttgcct gtggagatat gaccattgtc cggcgtgtcg gggatgacac
     4621 ggcggaagtg gcggaatatg tgcgtcgcat tcatgaggct gagttactgg accatatcgg
     4681 tattgacccg tcaggggtgg ggcagattct ggattcactg gcggaagccg ggatccccga
     4741 cggaattgtg gtggggataa gccagggctg gaaactgggc ggggccatta aaaccaccga
     4801 gcgcaaactg gctgaagggg tgctggtgca tggtgaccag cccctgatgg cctggtgtgt
     4861 cggcaatgcc cgggtggagc ctaaaggtaa cgccattctt atcaccaaac aggccagtag
     4921 acggggaaaa attgacccgc tgatggcgct gttcaatgcg gtctccctga tgtcccttaa
     4981 cccggaaccg aaaaagaaag aatatgcggt ttttttcata taaccctgtt cacactgtaa
     5041 ccatcacgaa ccgctccggc ggttttttta ttttcaggag gctgatgtga ctcttaaacg
     5101 ggcctgttcc ctgctgacgg tgaaatcctt cagtgaggat gaacgggtga tcaccgggat
     5161 tgcgtcaacg ccttctccgg atcgggatgg tgacatcctg gagccggagg gcgcggagtt
     5221 tggcagtgcg atcccgtttc tctggcagca tgaccattcc cgcccggtgg ggcagtgtac
     5281 ggtgcgccgg gtcagcgaag ggctggaaat cacggcaaca ctggtgaagc ccgtaccgga
     5341 tatgccgtcg caactggctg cccggctgga tgaggtctgg gcggccatta agaccgggct
     5401 ggtcaggggc tgtccgtggg cttccgtccc catgaataca cctttctgga cggaggcgga
     5461 ctgcattttc tgcgctggga actgatggag gtgtctgccg tcaccgtgcc cgcgaatgcg
     5521 gaatgcacca tccggaccat taaatcttac gacagcccgt tttctgccgc gtccggcaac
     5581 cggaaaccgg tggtgaaaat cgcatcttct gccggcgctg cggcacagtc aacaaccgtt
     5641 tttcataagg aaaagaccat aatgaatatt ggcgaacaga ttaaaagttt tgaaaacaag
     5701 cgtgtagcgc tggcagcctc ccttgaggag gtcatgacca aagccgcaga ggaagggcgc
     5761 acgctggatg tggaggagga agagcattac gacaacaccg cagcggaaat ccgtcaggtg
     5821 gatgcgcacc tgaagcgcct gcgtgaactg gaagccggta aggccgccac ggcgcagccg
     5881 gtgaaacagg ccggtaacgg gaatgtggcc gcggtggctt ctgcgccggt gatccgtgtg
     5941 gagcagaaac tggataaggg gattggcttc gcccgctttg ccaaatcgct ggctgcggct
     6001 aaaggcgtcc gatctgaagc cctggaagtg gcccgtcgtc agtatccgga tgacagtcgt
     6061 ctgcatcatg tcctgaaatc ggcagtgggc gcggggacca ccacggatcc gcagtgggca
     6121 ggcagcctgt ctgaatatca ggaatacgca caggacttta ttgattacct gcgtccgcag
     6181 accattatcg ggcgatttgg tcagggcggg atccctgcac ttcgtcaggt gccgttcaat
     6241 atccgtgtgc acgcccaggt gtccggcggt gctgccggct gggtgggtga gggtaaggca
     6301 aaacccctga cgaagtttga ttttgaatcc atcaccttca gtcatgcgaa ggtgtcggcc
     6361 attgcggtac tgacggaaga gctgatccgt ttttccagtc cggcagctga tgcgctggtc
     6421 cgtaatgcgc tggcagaagc ggtggtggcg cgtctggaca cagactttgt ggacccgaaa
     6481 aaagccgcag tggcagatgt ctccccggcg tccatcaccc atgatgtgaa gggcaaggca
     6541 tcaaccggta acccggatgc ggatgcagag gcggcgtttg gacagtttgt ggcagcaaac
     6601 ctgcagccca ccggtgcggt ctggctgatg tccagcacca atgccctggc gctgtccatg
     6661 cgtaaaaata cgctgggtca gaaggaatac ccggacatga ccctgctggg ggggaccttc
     6721 caggggctgc cggtgattgt ctcccagtac gtgggtgacc agctggtgct ggtgaatgcc
     6781 ccggatattt atctggcgga tgacggcggc gtggcagtgg atatgtcccg cgaggcatca
     6841 ctggaaatgc agtctgagcc gaccggcgac agtaccacgc cgtcgccggt ggagctggtt
     6901 tccatgttcc agacaggcag tgtggccatc cgtgcggagc gctggatcaa ctggcgtcgt
     6961 cgccgtaccg cggcggtggc ggtgatcacc ggtgtgaact acggcagtgc gtccggcggc
     7021 tgagtctgat aaggaggacg ggaggcgagc gcctcccgta acaggttatg gcaaagatcc
     7081 gatatctgca gggcacgcat gatgcccggg ccggggatat ccgtgatgtg gcacagccgt
     7141 gcgcggaggt gctggttcgc ctgggaaagg cggagtacat cacggtgcga cgtccggcag
     7201 gtcagaaaaa gaaacgtgat gcggagcatg gcgaatgtgg aaccttttac agcgaacccg
     7261 aaaaaaacag aaatcaggac gtgacgtaag agaggcgggc tggaccagcc tgtttcaggc
     7321 ggtggctgag cccttttccg gcgcctggca gcagggcgtg aaagccgatc cggaagccgt
     7381 cctctccttt catgcggtgt tttcgtgcat ttcgctgata tcccaggata tcgccaaaat
     7441 gcggctgcgt cttatgcaga cggatgcgca ggggatacgc agggaaactc gacgggggga
     7501 tattgcccgc ctctgtcgtc gtcccaacgc ccagcagaac cgcatccagt tttttgaact
     7561 gtggctgaac gccaaactgc gtcacggcaa tacggtggtg ctgaaaatcc gtaactcccg
     7621 ggggcagatc aaagaactgc gcattctgga ctggagccgg gttgaaccac tggtggcgga
     7681 tgacggcgaa gtgttctacc gcatcacgcc ggaccggaac tgcgggatca cttaggcggt
     7741 gacggtgcct gcccgggaag tgatccacga ccggtttaac tgtttttttc atccgcttat
     7801 agggttgccg ccggtgtatg ccgccgggct ggccgccacg caggggcatc atattcagga
     7861 aaattcgacg tattttttca gaaatggcgg catgccgtcc ggggtgattg agatccccgg
     7921 cagtattacg gaagaaaatg cgaaaaaact gaagagcaac tgggacagcg ggtataccgg
     7981 cgaaaatgcg gggaaaacgg ccattctgag caacggggca aaatacaacc ccacgacgtt
     8041 ttcaccggtg gatgcgcaga cggtggaaca actgaagatg accgctgaaa ttgtctgttc
     8101 ggtgttccgt gtcccggcct acaagattgg cgtgggacaa ccgccttcca gtgacaacgt
     8161 ggaggcgctg gagcagcagt attattccca gtgcctgcag acgctgattg agtccattga
     8221 actgttactg gatgaggcgc tggaaacggg ggaaaacgag agtacagaat ttgatgtcac
     8281 cacgctgctg agaatggaca gtgagcggcg catgaaaacg ctgggggatg cggtgaaaaa
     8341 tacgcttctc acgcccaatg aggcccgtaa acgggagaac ctgccgcccc tggccggcgg
     8401 tgatgcactg tatcttcagc agcagaacta cagtctggag gcactgtccc gtcgtgatgc
     8461 ccgtgaggat ccgttcgcgt cgaccgggaa aacggcctta gcgcaaccgc ctgacggcgc
     8521 atctgacggt aataaggcaa tcagtgaaac agagcatgat gcggtgaaag cgatgttcag
     8581 ggggattctg aaaaaatgaa tgaacgtgaa ctgtccatta ttcgtgccct gggagaagaa
     8641 ttcgccacgg tgctggcgga tttacagcgc acatttgagg agaagatgag cgcgcaggca
     8701 caagcgtttg aagagaaact ggcttccctg tccgctctgt tacagaagca tgtgacggtg
     8761 gatgaggtta gccctgttct gcaggcgatg gtggatgacg cagtgggggc cattccgcta
     8821 ccgcgtgatg gtcacgatta tgatccggaa gtactgaagc aggcggtgaa tgatgcggtc
     8881 gcgaaaatac cggtgccggc agacggcaaa agtatcaccc ccgatgatgt gcgtccgatg
     8941 cttgagcaga tggtgaaaga ggctgtaagc catattcctg ttccgcgtga tggtcgtgac
     9001 tacgatccgg aagtactgaa gcaggcggtg aatgatgcgg tcgcaaatat tccgcagccg
     9061 gcggacggta aaagtctcac cccggatgat gtgcgtccga tgcttgaaca gatggtgacg
     9121 gaggctgtaa ggcatatccc tgttccgcgt gatggtcgtg actacgatcc ggatgttctg
     9181 cagaaggcgg ttctggatgc ggtgagtgcc ctgccggctc cgcaggacgg gcgtgatgcc
     9241 acggctctgg aaatactccc cgccattgac gttcaaaaat cctttccccg gggcacgtat
     9301 gccacacacc agggcggact ctggcgggcg tatgaaaaaa cgcacgggat gcggggatgg
     9361 gaatgcctgg ttgacggggt ggcggatatt gacgtcagca tgacgggtga gcggttgttc
     9421 tctgtggtgg tccggcagag cagtggccag cgtacggaaa aaacattttc cctgccggtg
     9481 atgctctacc gcggtgtgtt cagagccggt gaaacctacc accccggcga tacggtgacg
     9541 tgggggggct cgctgtggca ctgcaacagt atgaccgaag ataaacccgg agaagctcat
     9601 tcatcagcct ggaccctggc tgcaaaacgt gggcgggatg caggaggcgg aaaatgacgg
     9661 cattactgac actggaagag gtcaaggcac atctgcgtgt cgaccatgac gcggatgatg
     9721 acatgctgat ggacaaggtt cgtcaggcta ccgccgtgct gctggcctac attcagggca
     9781 gccgggataa agtgatccgt gaggacggtg aactgatccc gggcgaggca ttaacccgga
     9841 tgaagggggc tgccatgcga ctgaccggga tgctgtaccg gaatccggat cttgcggagc
     9901 gggaagaact gcttcagggg gagctgccgt tttctgtttc cgtgctgatt tacgatttgc
     9961 gttgtccgac ggtgttatga ggagggggaa tggcaatatc tgtaggtcgt ctgacacaga
    10021 tgataagtgt tctgaacccg gtgttaaccc gtaacgctgc cggagaaatg acggaagaat
    10081 gggtgtcatg cgggaaaatt catgcggata tccgaggcag gagcagccgg gagcggatgc
    10141 agttcggtgc ggaaatggcg caggcggaaa tccgcatctg ggtgcgcggt cagtccggtc
    10201 gggaaatcac ggcagcgtca cgacttcatg tgctgagtgg tccatggcgt gaccggatcc
    10261 tgaacgttgt cgggctgccc gtgccggatg cgaccggcgg gcgtctggaa attctctgtc
    10321 ggctgggagg ggaaaaatga tcgaaatcct gctggatttt tcggggctgg aggacatcag
    10381 ccgcgatttg cagcttctga gtggtgcgga aaataaccgg gtgctgcgtg aggcaacccg
    10441 tgcgggtgcg aatgtgctga aagaagaagt ggtgttacgg acaccggtac gcaggggaaa
    10501 actgcgccgc aatgtggtga tcctttcccg gcgctcccgc gatggcggga tggaatccgg
    10561 tgtccatatc cgtggtgtta atccggacac cggtaacagc gataacacca tgaaggcgga
    10621 taacccgcgc aatgctttct actggcggtt tgtggaaatg gggaccgtga atatgccacc
    10681 gcacccgttt gtgcgcccgg cgtttgatgt gcgcagtgaa caggcagctc aggtggcgat
    10741 tgcgcggatg aaccgggcca ttgatgaggt actgagacga tgacggaggc ggatttgtat
    10801 cctcatctgg cgcatcttgc cggcgggcag gtgtacccgt atgtggcccc cctgctggat
    10861 ggcaggccgt tggtggcgct tccgtgggtg gttttcagcc tgatttcatc ggtgtcagcg
    10921 gacgtgatgg gcgggcaggc ggagtcctca gtgtcggtgc agatagacgt ttatgccggg
    10981 actgtgacgc aggcgcgtca gatacgtcag gacgcccgtg aagccataat gctgctggcc
    11041 ccgggatccg tcagtgaaat gcaggactat attccggaaa accgctgtta ccgtgcaacc
    11101 ctggagtttc aggtcacggt gtgacttttt cttttttcta caaaaccata ccccgccgcg
    11161 tgcgggtttt ttattatcag gaggcagaat gtctgctttg tatgaacgct cacagctgac
    11221 gcaggtgatg atttcatctg ccctggcgac tgctgaaact atggataagg cggaatatct
    11281 gcgcctggac tgcaccatca aggaagtcca gttcaccgcc ggtcagaaac aggatattga
    11341 tgtgaccacg ctctgctcca cagagcagga gaacatcaac ggtctggggg cgtcgtctga
    11401 gatttccatg tcgggtaatt tttatctgaa tcaggcccag aacgccctgc gtgatgccta
    11461 tgacaatgac gcgttgtatg cgtttaaggt gctgtttccg tccggtaagg gctttaaat
//
LOCUS       DAJTNA010000127        11459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTNA010000127 DAJTNA010000000
VERSION     DAJTNA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11459
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.128"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            106..882
                     /gene="exuR"
                     /locus_tag="OO161_002900"
     CDS             106..882
                     /gene="exuR"
                     /locus_tag="OO161_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5463895.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGQQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1227..1889
                     /locus_tag="OO161_002901"
     CDS             1227..1889
                     /locus_tag="OO161_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5463896.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1893..2276
                     /gene="mzrA"
                     /locus_tag="OO161_002902"
     CDS             1893..2276
                     /gene="mzrA"
                     /locus_tag="OO161_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5463897.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2423..2791
                     /locus_tag="OO161_002903"
     CDS             2423..2791
                     /locus_tag="OO161_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR5463898.1"
                     /translation="MKDRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2829..3134
                     /locus_tag="OO161_002904"
     CDS             2829..3134
                     /locus_tag="OO161_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5463899.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRAAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3137..3541
                     /locus_tag="OO161_002905"
     CDS             3137..3541
                     /locus_tag="OO161_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5463900.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3531..3830
                     /locus_tag="OO161_002906"
     CDS             3531..3830
                     /locus_tag="OO161_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5463901.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4016..4408
                     /locus_tag="OO161_002907"
     CDS             4016..4408
                     /locus_tag="OO161_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5463902.1"
                     /translation="MKKLEDVGVLVARILMPILFMTAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSKFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4478..5422
                     /gene="yqjG"
                     /locus_tag="OO161_002908"
     CDS             4478..5422
                     /gene="yqjG"
                     /locus_tag="OO161_002908"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5463903.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGITETVNFDHIRNHYFRSHKTINPTGIISIGPW"
     gene            5757..6122
                     /locus_tag="OO161_002909"
     CDS             5757..6122
                     /locus_tag="OO161_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5463904.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6364..6720
                     /locus_tag="OO161_002910"
     CDS             6364..6720
                     /locus_tag="OO161_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5463905.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(6771..7667)
                     /gene="yhaJ"
                     /locus_tag="OO161_002911"
     CDS             complement(6771..7667)
                     /gene="yhaJ"
                     /locus_tag="OO161_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5463906.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     SDSMGEAKSWCLREIPKLFSGK"
     gene            7772..8473
                     /locus_tag="OO161_002912"
     CDS             7772..8473
                     /locus_tag="OO161_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5463907.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESTNFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8496..8660
                     /locus_tag="OO161_002913"
     CDS             8496..8660
                     /locus_tag="OO161_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463908.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8794..10104)
                     /locus_tag="OO161_002914"
     CDS             complement(8794..10104)
                     /locus_tag="OO161_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5463909.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALVAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10133..11454)
                     /locus_tag="OO161_002915"
                     /pseudo
     CDS             complement(10133..11454)
                     /locus_tag="OO161_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         2906 a         2985 c         2782 g         2786 t
ORIGIN      
        1 aattcaatac attaattcaa tcaccaaatt tatatatgac caatggcata cccatgaata
       61 aagtggtatg ataacttcgt ccgcattcca ctccgggaga cgcatatgga aatcactgaa
      121 ccacgccgtt tgtatcaaca acttgccgct gacctgaaag agcgcattga acagggcgtc
      181 tatctggtgg gtgataaact gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc
      241 cgaacggtag ttcgtgaagc cataatcatg ctggaagttg aaggctatgt ggaggtgcgt
      301 aaaggttcag gtattcatgt ggtttccaac cagccgcgcc atcagcaggc ggctgacaat
      361 aatatggaat tcgccaatta cggtccgttt gagttgcttc aggctcgcca gctcatcgaa
      421 agtaatattg ccgagttcgc ggcaactcag gtaacgaaac aagacatcat gaaactgatg
      481 gccattcagg aacaggcgcg cggccaacaa tgcttccgtg attccgagtg ggatttacag
      541 ttccacattc aggtagccct ggcgacgcag aactccgccc tggcggccat cgttgaaaaa
      601 atgtggaccc agcgtagcca taacccgtac tggaaaaaac tacacgaaca cattgattcc
      661 cgtaccgttg ataactggtg tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa
      721 gatccccatg ctgctaagct ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta
      781 tttaacgaaa ccagcgacga tttcgagttc aatgccgacc gctatctatt cgccgaaaac
      841 ccggtagtgc atctcgatac ggccaccagt ggcagtaaat gaaactcctg tccgacaggc
      901 gctgcgacgc gcctgttcgc ttcgtcggta agcgaaaagt ataaagtgtc agcctgtgta
      961 aatcctctcg ccaccctccc ctgcattcag caaaatcaga ctccacggac atgtaatttt
     1021 gataacgaac aacgttgacc tttgttacaa ttagattcaa tttgaattta tgtttttgaa
     1081 tgctttctta tctcacgatt taacagggaa tagttcaggc tgtgttgatg tatcaaaccc
     1141 gcagaacata ccaaaacagt aataacattg cggtagtgca tcttttaaaa ccagcgtggc
     1201 gttaaccgat tcaccaggaa taatgaatgg aacttttgac ccaattgctg caagccctgt
     1261 gggcgcagga ttttgaaacc ctggccaatc catcgatgat tggcatgttg tattttgtct
     1321 tgtttgtaat tttgttcctt gaaaacggct tgcttccagc ggccttttta ccgggcgaca
     1381 gtttactggt attggtcggc gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga
     1441 ttctgctgct gaccgttgcc gccagcctcg gctgctgggt cagctatatt caggggcgat
     1501 ggctgggcaa tacccgcacc gtacaaaact ggctatctca tttacccgcg cattatcatc
     1561 aacgcgcaca ccatcttttt cataaacacg gtttatcggc gctgttaatt ggtcgcttta
     1621 ttgcgtttgt cagaacattg ctgccgacga ttgccgggtt atcagggctg aataacgcgc
     1681 gctttcagtt tttcaactgg atgagcggtc tgctgtgggt attgatcctg acaactctgg
     1741 gttacatgct cggcaaaacg ccggtatttt taaagtacga ggaccagctg atgtcatgcc
     1801 tgatgctgct cccggtggtg ctgctggtgt ttggcctggc aggttctctg gtcgtgttat
     1861 ggaaaaagaa atatggaaat cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc
     1921 agctagcctg gtccggcact gttttacttc tggtcggcac gctgttgctg gcctggtccg
     1981 cggttcgcca gcaagagtct acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc
     2041 cagacggttt ttcgatctgg catcaccttg acgctcatgg cattcctttc aaaagtatca
     2101 cccccaaaaa cgacactctg ttaattacat ttgactccag cgaccagagc gccgccgcaa
     2161 aagcggtcct cgacagaaca ttgccccatg gctacatcat tgcgcagcag gacaataaca
     2221 gtcaggctat gcagtggctg acccggttac gggataactc tcatcgcttc ggataacttc
     2281 ctgttatccg aaacatatca ctcactttgg tgatttcacc gtaactgtct atgattaatg
     2341 aggcggtggt taacactacc gcttcgttgt ctggacaatc gttcctttgt aataggtccg
     2401 aatcacaatg gaaggttcaa gaatgaaaga ccgcatcgct ttagctgttt ctctctttgc
     2461 tcttagtgcc ggtagttatg ccactaccct gtgtcaggaa aaggagcaaa atatccttaa
     2521 ggagatcagc tatgccgaaa aacaccaaaa ccagaatcgt attgacggtc tgaataaagc
     2581 cctgagtgaa gtccgggcca actgttcaga tagccagctg cgtgccgatc atcacaagaa
     2641 aatcgcaaag cagaaagatg aggtggcgga acgccagcaa gatttagccg aggcgaagca
     2701 aaaaggcgat gccgataaga ttgccaaacg cgaacggaaa ctggcagaag cgcaggaaga
     2761 gctgaaaaag ctggaagcgc gcgactacta actcacaata gtcactactt actcacctgg
     2821 agaaaactat gtcgaaagaa cacactacgg aacatctgcg tgctgagttg aaatcccttt
     2881 ccgatacgct ggaagaggtg cttagctcat ctggcgagaa gtcgaaagaa gagttgagta
     2941 agattcgtag caaagcggag caggcactga aacagagccg ttatcgcctg ggtgaaaccg
     3001 gtgatgccat cgccaaacaa acccgtgccg cggcggcgcg tgctgatgag tatgtgcgtg
     3061 aaaatccgtg gaccggcgtg ggaattggcg ctgcaatcgg tgtagtgctc ggcgttctgc
     3121 tgtcgcgtcg ttaattatgg cggacactca tcacgcgcaa gggcccggta aaagcgttct
     3181 gggcatcggg cagcgaattg tttccatcat ggttgaaatg gtggaaacac gtctgcggct
     3241 ggcggtggtg gagctggaag aggaaaaagc taatctcttt caacttttac tgatgctggg
     3301 cctgacgatg cttttcgctg catttggtct tatgagcctg atggtgctaa ttatttgggc
     3361 ggttgatccg caatatcgcc tgaatgcgat gattgccacc accgtggtgt tgctgctact
     3421 ggcactgatt ggcggtatct ggacgttacg taaatcgcgt aaatccacct tgctgcgcca
     3481 tacacgccat gagctggcaa acgatcggca actgctcgag gaggagtccc gtgagcagta
     3541 aagtcgaacg tgaacgacgt aaggcgcaac tgcttagcca gatccagcaa caacggctgg
     3601 atctttccgc cagtcgtcgt gaatggctgg aggcaacagg cgcttacgat cgtcgctgga
     3661 atatgctgct aagtctgcgc tcctgggcgc tggttggcag tagcgtgatg gcgatctgga
     3721 cgattcgcca tcctaatatg ctggtccgct gggccagacg cggttttggc gtatggagcg
     3781 cctggcgtct ggttaaaacg accctcaagc agcaacagct tcgcggttaa cttccctctg
     3841 gccggagcca ttccggcctt atccctcaaa ttttttgaag atttttgaca gttttccttg
     3901 ctaacaatca tcatttacca cgtttatgat tctctccatc gacagcaacg acgctaatac
     3961 cgcgcccttg cacaaaaaaa caatcagcag cctgagtggc gcagtggaga gtatgatgaa
     4021 aaaattagaa gatgttggtg tactggtagc gcgcatttta atgccgattc tgtttatgac
     4081 cgcaggctgg ggaaaaatca ccgcttactc tggtacccaa caatatatgg aagcaatggg
     4141 tgtcccgggt tttatgctgc cgctggtgat tctgcttgag ttcggtggtg gtctggcaat
     4201 cctgttcggt ttcctgactc gcaccacagc cctgtttact gcgggcttta cgctgctgac
     4261 ggcattttta tttcacagca agtttgctga aggcgtcaac tcgctgatgt tcatgaaaaa
     4321 cctgacaatt tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat
     4381 cgaccgcctg ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg
     4441 aggaagctcc tcgtttttgc tattggagga gagaaaaatg gggcaactga ttgacggcgt
     4501 ctggcatgac acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc
     4561 cgcatttcgt aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt
     4621 tatcgcagag aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg
     4681 cacgctgatc atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa
     4741 cccgctgatg ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga
     4801 tacgctctat caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag
     4861 cggacgagtt actgttcccg tgctgtggga caaaaagaac cacaccattg tcagcaacga
     4921 atcagcagaa atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg
     4981 tgattactac ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga
     5041 caccgttaac aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga
     5101 ggcggtggcg aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg
     5161 ttacctgacc ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cactggtgcg
     5221 ttttgatcca gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct
     5281 gaatctgtat ggcttcctgc gcgatatcta ccagatgccg gggatcaccg aaactgtcaa
     5341 tttcgatcac atccgtaatc attacttccg cagccataag accatcaacc ctacggggat
     5401 tatttcaatt ggtccgtggt aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg
     5461 ttaaaaaaag gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt
     5521 cggatgcggc gtgaacgcct tatcaaacct acaaaacgtt tatattcaat atgttaaaat
     5581 gaccgcgtag gccagataag cgtagcgcat ctggcgattt tgcgcttgtc atcagtctta
     5641 gcgcaccttc acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac
     5701 gtctattctt aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg
     5761 actggtatct gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag
     5821 agtactggat gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata
     5881 aaatgttagg ctggcaacgc gccggtggcg aaggcatcct gacgacaatc tacggtattc
     5941 tggtgttttt accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg
     6001 cgtggtgggc actgttgttc ttaatcccgt ttatcggctg gcttattatc atcgtcttta
     6061 actgccaggc gggtacgcca ggcgaaaacc gctttggacc agatccgaaa ctggaacctt
     6121 aaaacaaatc tgctcgccgg atgtctgact catccggctt ttatcatcat gttgatgaat
     6181 gaataattta ctaatgttta tttaaaatat ttcaatgtcg ttatataaat tacctataaa
     6241 aaacaaccat gtatttatct taaatatcaa aacctttata acatccgaaa aagaaagata
     6301 cgaatagcat ttacacaata cccggaatag actttcgtca cttttaatta aagggatgtt
     6361 tttatgcagt ggtatttagc cgtattaaaa aattatgttg gtttctctgg tcgcgcacgt
     6421 cgtaaagagt actggatgtt tactctgatt aacgccatcg tcggcgctat tatcaatgtc
     6481 attcaattga ttttaggttt agagtttcca ttcctttctc tcatttacct ggcagcaaca
     6541 atcattccgg tcattgctct gtgtgttcgt cgtctgcacg atactgaccg ttcaggtgcg
     6601 tgggcgcttc tctatctggt accgatcatt ggctggttag ttctgtttgt atttgcctgt
     6661 ttagaaggca attctggcag caatcgttac ggaaacgatc ctaagtttgg ttcaaattaa
     6721 tcttagaatt ggggcgatat ttcgcccctt tttattaaca ataataaata ttattttccg
     6781 ctaaaaagtt tgggaatttc ccgcagacac caggatttcg cttcccccat actgtcacta
     6841 cgccaggcca taataatatc gatctcgctg gtcgattccg ggctgacgac acgcaaccgc
     6901 ccttccgcaa tatctttttc gaccatcgga tacggcatcg tcgccacgcc aagccccgcc
     6961 agtaatgcct gacgtttatc ttcaatcgtg ctcaccgtta agcgcggctg tttgtccagc
     7021 agctgtacgg tcaacaccgg gcgctcacga gcggtatccg ctaccgcaat tccacgatat
     7081 ttcacgcgcg tcacttcaga taacggttcc ggctcctgat gaatcgggtg atcaggcgcg
     7141 gcaacgtaga cgttcattaa ggtatagagc ttgcgcgagt tgatctccga cgaggaacga
     7201 aaatgcatat ccggcgcgat aacgatatcc gcccgcccct gctccagtcg ttcccacgcc
     7261 cccgccagca cttcggtgat gattgccagt tgggtattgg cttttgccgc cagtttgtcg
     7321 attaacggga aaaaggcagg tgtcggtacc agcgcctcgg tcacaatggt gagatgcgtt
     7381 tcccaaccgc gcgcgagggc ttccgcatcg gtagtcagtt tatctgctgc ttccagcaaa
     7441 acgcgccctc gctccagcaa catcctcccg acattggtga atttggtgcg atggcccgag
     7501 cggtcaaaca gcaccacatc cagctcttct tccagttttt gcatggtgta gctaagtgcg
     7561 gaaggcacgc gtcccagctc atccgccgcc gccgcaaaac tgccccggcg atcgatcgca
     7621 tccataaccc gtagtgcttc cagcgttaat gccctttctt tggccatttc gttctcattc
     7681 aggaaatttg aacataccgg gcagaatatc tggctaacaa tgtagcgtcc agccccttac
     7741 cataaaagga agtaaagaga ggtcaagaat tatgattact acccgaactg ccaggcagtg
     7801 tggacaagca gactacggat ggttgcaggc ccggtatact ttttcctttg gacactactt
     7861 cgacccgaaa ttgttaggct atgcctccct gcgtgtgctt aaccaggaag tgctggcccc
     7921 aggtgccgcc tttcagccgc gaacctatcc caaagtcgat attttaaatg tgattctgga
     7981 tggagaagca gagtatcgcg acagcgaagg caatcatgtt caggccagcg ccggtgaggc
     8041 gttgctgctc tctacccagc cgggtgtcag ctacagcgaa cacaatctca gcaaagacaa
     8101 accgttaaca cgaatgcagc tttggctgga cgcctgcccg cagcgagaga atccgctgat
     8161 tcaaaaactg gcgcttaata tgggcaagca gcaattaatc gcctcgccag agggtacgat
     8221 gggaagcctg caattacgcc agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga
     8281 aagtacgaat ttccagttgc atggaccacg cgcgtatttg caatcgattc acgggaaatt
     8341 tcatgcgctt acgcatcatg aagagaaagc ggcgctgacc tgcggtgatg gggcgtttat
     8401 tcgtgacgag gctaacatta cgctggttgc cgattcccca ctgcgcgctt tgctgataga
     8461 tttgcctgtc tagttgtttt tacagggaga tgatgatgag taaaaaatcg gccaaaaagc
     8521 gccagccagt gaagcccgtg gtggcgaaag aacctgctcg caccgccaaa aattttggct
     8581 atgaagagat gttgagcgag ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg
     8641 aggatgaagc taccgcgtaa agacaggttg ccggatgcgg cgaaaacgcc ttatccggcc
     8701 tacaaaatcc aacaacatta acgaattaaa caacccgtag gccggataag gtgcgccagc
     8761 atcgcatccg gcacaatact caaaacctgg cgtttatctg gccttgctcg ccataatctc
     8821 gataatctgc cgatccgttt gctgcatcga atggcttgct aacgcacaca ggttggcaat
     8881 cgactgctca acatcatgcg ccacgatccc ttcattgccg gtcacggcgg tatcatccag
     8941 cgccattaac accgctttcc acgcagccga agcactggtc gaaaccttca tcgcgcagct
     9001 gttcgacgca ccatcgcaaa tcatgccgct gacatcgccg atcatactgc tgatcgccat
     9061 cgagatagtt tcataacgcc catccaccag ccatgccatc ccggcggcgg cccccattgc
     9121 tgcggtcgtt gcggcacaaa gtgcagacaa acgcggtaac tggttatgga tgtaaattgc
     9181 gctcaaatga gaaagcatca gcgcacgcgc cagtcgttca tcatccgctc cgaagtgttc
     9241 tgctacaacc accacaggca ttgttgcggt gatcccctgg ttacccgagc cggagttact
     9301 catagccgga agcgtagcgc cgcccatacg cgcatcggat gccgcgctgg tacgaatcac
     9361 aatggatgaa gagagatctt tcgccagcaa accgcgctcg cactgttttt ccagcgtcgc
     9421 gccaatatgc agcccccact taccgctcaa accttcctgc gataacgcac aatttagctt
     9481 cgcggaatcg agaataaagc ggatcgccgc aaacgggact tcattgacga acttcaggat
     9541 ctcagccagc gtcgttctgg aaagcaccgt cagcggagac tcttgctcgc cctctgccac
     9601 acacgcctgc tgggtaaaca ccacactatt gtgcgtctca atatgcacaa tgttggtatg
     9661 cccgccgacg atggtgacac acgcccactt ctcaccgttc cagactttgg cgcgtgagaa
     9721 gaggatttca ttgcaaggtt cctggatctt aacggagact ttccccgccg ccagcagtgc
     9781 tttggcatcg gcaattgcct gcgcagttgc gtctttcagc acttccagcc cggcgttggc
     9841 atttccacct aacgccccca gcgccgccgc aatcggcagc cccaccattc ccgtgccggg
     9901 aacggtgacg cccagaccgt tcttcatcag atttggcgaa acccaggctt ctacacgttc
     9961 aaccggacct tccagttctg ccgcagcaac cgccgccacc agcgccagtg aaatcggttc
    10021 agtacatccc agcgccggtt ttacttcctc ctgaacggcg aggatgtaac gctgccataa
    10081 cggatttaaa gtcgaatcaa acatattaaa aaaccttaaa atttcaggta aatcaagaaa
    10141 atgccaggaa cggagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca
    10201 tccctttgta tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc
    10261 ctaccatgcc gaaaatcggg ctacagatag aggtgaagct caacaccggt gcgttcagta
    10321 cgatggcgct ccaggccagc aaaatggcga aaatcatgat gccgcgctga acaagatttt
    10381 cgttaatctt ctcggcaggc atcttgcgac gcaggatgtt cattacgatc ccttgcgttg
    10441 cttcgcgaaa gcctaaatag acgccaaaga atgcagtcat gacagcaaag atattgagaa
    10501 tgacgctgac cactttcacc cacgctgcgc cgtcaccgct aataaactgt gcggcaatcg
    10561 ccagcgcgga aatattctgc tcgtaggctt ttaccgcttc gtcatgtccc atcgccagcg
    10621 tgaacgacac ggcgtagaaa aagacggtga caaataaaat gccaaacgcg atattcattg
    10681 cccgcaatgc tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca
    10741 ccatcgggct taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa
    10801 tggcgttttt caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca
    10861 ttcccaccat cgacacgccc agcgcagcga ctaccagcag cttggtcagc accatgccgg
    10921 tcgaaatttt gaataacaat ttctcgccgc gtgaagagat cgccaccaga atgcaaatca
    10981 gtaccagacc ataaaaggga ctgtctgaca gcaacccttc cgtcacaccg aaggtatgca
    11041 gataggaagc actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca
    11101 ttacgaaata gagcgcacct aacaggatgc cccagttttt acctaaataa ccgctaatga
    11161 cactcgggta atctttacat tctggtgatt ctgccagcgt attaataaac aaccgctgaa
    11221 acagatacat tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca
    11281 aaccgacctg caccgggaga aaaacacaat cgccatcccg atactcataa tcacccagcc
    11341 ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca ttccagcacg
    11401 acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt ccataattt
//
LOCUS       DAJTNA010000128        11388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTNA010000128 DAJTNA010000000
VERSION     DAJTNA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11388
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.129"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..963)
                     /gene="aldB"
                     /locus_tag="OO161_002916"
     CDS             complement(<1..963)
                     /gene="aldB"
                     /locus_tag="OO161_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5463910.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYE"
     gene            complement(1128..2279)
                     /gene="yiaY"
                     /locus_tag="OO161_002917"
     CDS             complement(1128..2279)
                     /gene="yiaY"
                     /locus_tag="OO161_002917"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5463911.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(2469..4313)
                     /gene="selB"
                     /locus_tag="OO161_002918"
     CDS             complement(2469..4313)
                     /gene="selB"
                     /locus_tag="OO161_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5463912.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(4310..5701)
                     /gene="selA"
                     /locus_tag="OO161_002919"
     CDS             complement(4310..5701)
                     /gene="selA"
                     /locus_tag="OO161_002919"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5463913.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(5799..6407)
                     /locus_tag="OO161_002920"
     CDS             complement(5799..6407)
                     /locus_tag="OO161_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5463914.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            6635..10599
                     /locus_tag="OO161_002921"
                     /pseudo
     CDS             6635..10599
                     /locus_tag="OO161_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
     gene            10596..11100
                     /locus_tag="OO161_002922"
                     /pseudo
     CDS             10596..11100
                     /locus_tag="OO161_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11165..>11388
                     /locus_tag="OO161_002923"
     CDS             11165..>11388
                     /locus_tag="OO161_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5463915.1"
                     /translation="MTLAYRSLRIIHLYHCDYRGLPLTLISPDGATEWCAEYDEWGNL
                     LNEENPQHLQQLIRLPGQQYDEESGLYYNR"
BASE COUNT         2757 a         3105 c         3025 g         2501 t
ORIGIN      
        1 ttcgtagata gattcctgca ctaaagcacg actcggacag gtgcaaactt cgccctggtt
       61 aaaggcaaac agtgcaaagc cttccagcgc tttatcgaaa aaggcatctt cttcatccat
      121 cacatcagca aagaagatat ttggcgactt accgcccaac tccagcgtca ccggaataat
      181 gttttgcgtt gcgtattgca taatttgttg gcccacttcc gttgagccgg taaacgccac
      241 tttggcgatg cgtttcgagg tcgccagata ttcgccaatt tccccacctg cgccattgac
      301 cacgttcacc acgcccggcg gcagtaaatc accgacaatt tccattagca gcagtacaga
      361 aagcggggta agacgtgcgg gtttcagcac cacacagttg cccgccgcca gcgcgggagc
      421 cattttccag ctcgccatca gcagcgggaa gttccacggg ataatctgcc ccaccacgcc
      481 taacggttca tggaaatgat aggccacggt ttcgctatca acttcactga tcccaccttc
      541 ctgcgcccga atacacgagg cgaaatagcg aaaatgatca atcgccagcg gcacgtcggc
      601 agcactggtt tcgcgaatgg gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc
      661 gaggttttgt tccattcgat cggcaatctt aaacagaatc gccgcgcgat cctgcaccga
      721 ggtgtgcgcc catttatctt tcactttgtg cgcagcatct agcgccagat cgatgtctcg
      781 tttgcccgaa gacgccactt cgcacagcag ctgcccagtc accggcgtca ggttctggta
      841 atactcacca tcggcagggg ctacccattc gccgccaata aagttgtcat agcgggcttt
      901 taacttgagg gggaaaccat actcgccggg cttaatctgt gctgaagggg gattattggt
      961 catgatcgtc tccttgcggt gtgaggtata acaagggtag acgtgactgg cgaaatcttc
     1021 gccagtcggt aacagcttta cgacggctat cacgaattta cgggcaaggg ttacagagat
     1081 agttgaaaaa gcggctgaca atttgccagc cgttgtggaa atgatgatta catcgctgcg
     1141 cgataaatcg ccacaatttc ttcgtgagtt gcctggatcg ggttagtaaa accacaggca
     1201 tctttcaggg cattagtcgc cagaaccgcg aaatcttctt ctttcacgtt caggtcgcgt
     1261 aggcctgccg ggatatccac tttcttcgcc agttcacgga tggcgttaat gcaggcttca
     1321 gcaccttccg cgtcgttttt acctgtcacg ttcacgccca ttgcagcggc acagtcacgc
     1381 agacgtgcag cggcgacttt gctgttgaat acctgaacat ggggcagcag aacggcgtta
     1441 catacaccgt gtggcaggtt gtagaaaccg cccagctggt acgccatcgc atgaacataa
     1501 cccagagaag cattattgaa cgccataccg gcaaggaact gggcataagc cattgcttca
     1561 cgcgctttcg cattactgcc atcttcaacg gctaacggca ggttttcggc aatcatggtc
     1621 acggctttca gtgcacaagc gtcagtgatc ggcgtggcgg caatagaaac atatgcttcg
     1681 atagcgtgcg ttaaggcatc catacccgtt gcggcggtca gtgacttcgg cataccaatc
     1741 atcagagagg agtcattgac agaaagcagc ggagtgacat gtttatcaac aatcgccatt
     1801 ttgatatgac gcgcttcgtc agtgatgatg cagaaacggg tcatttcaga ggccgtaccc
     1861 gcagtggtgt tgatggcgat catcggcagc tgcggttttg cagagcggtc aacgccttcg
     1921 taatcgcgaa tatcgccgcc attggctgcc accagcgcaa taccttttgc gcagtcgtgt
     1981 ggagaaccac cgcctaagga gatcacgcta tcgcaattat tctctttaag taatttcaaa
     2041 cctgcggcga cgttttccgt ggtggggtta ggttgggtgc catcataaat aacgctaaaa
     2101 atattgcgtt cttccagtgc tttttgcaca tcgcccgcca tacctaattt cgttaacata
     2161 ctgtcagtga caattaaggt acgggtaaat ccataatctg ccatcatatt cattgcatca
     2221 gtcaatgaat cagcgccgat gacattcaca gaaggaataa agaaagttga agatgccata
     2281 acacactcct gaaagtgtaa aaaagggtgg gaagcttact gaggattttc attcgaaaat
     2341 atgatcatta ttattaatta tcaaaattaa tctaataaaa agcacattgt attcattaaa
     2401 caatgtgctt tttttaaaat cataagaaat ttgtattaat ttattttaaa aattaattaa
     2461 tcatttcctt atttttccgg aaataataat gcgtcgcgca ataaatgatc atttccacga
     2521 cgacgcgtaa agccaatgcg gtcaaaatat tccagaatct gaattgccag ctttcggcct
     2581 acgcctaagc gatcgcggaa atccgccgcg caggttgaac cacactcctg atcgagatcg
     2641 cggatcatat tggcaaattc gacaatccga tcgttacggt aataacgatc tttgacgatc
     2701 gcggtaatta ttccttgctg cgccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg
     2761 gtacccgtct cttttgccag atcacgcacc caccacggct cgtcaccaaa cagcggctct
     2821 gctttttgcc agatggcctg ctgctcttcg ctgaagcccg ctttgtgatc cggcagatgc
     2881 agccaaccgt gatgactgag gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt
     2941 actagcgctt catcttccat tggcaacgcc atacgtcgca gacgttcgcg cccaggccca
     3001 ggttcatcgc gatgttgctc atgataagtc gctaatgtgt cgagaatttt ccgttgccag
     3061 cgagcggcaa ccggcgcatt caacaagcta taaccagcct gaatatagcc aggctgttgc
     3121 agcagttcgc gcatcccttc gccgttgagc tggcgcgccc aggcgaaatc aggaagatta
     3181 accgcgccgc gttgcaggtg aacggctaac gcttcgctat cattctgtgc cctggcgagg
     3241 gaagccagcc attgcagata ctctggttta cgtttaccgc gacgcggcgg gttaagcatc
     3301 acgacgcgcg ctccggccag cgtgttgcgg gcagagatat cgcgcaatac caggcggtcg
     3361 ttatctgcca gccataacgg ggtgtcgaag accagttcag caaggttatc ttccagcagt
     3421 gaaacgcgtc ccgtgacgtg gctggcggcg tggtgaatat gcagcggctg ccactgggtc
     3481 agcggtgtat gggtttgaag ctcgacaatc acccgtgtaa acggctctgg cggcgcatcg
     3541 gcaagcagcc agtcgccacg gttaatctgc tctttttccg catcacccgc gatgttaagc
     3601 gcgatacgct gcccggcatg ggcggtttct gttggctggt tttgtgcatg cagcgcacgt
     3661 acacgcatcg gtttatttac accagtcagc cagagtgaat cgcctacctt cacttccccg
     3721 cttaacgccg taccggtgac gaccagcccg gcacctttta cggtaaatgc gcggtcgatc
     3781 gcgaggcgga aactatgttg gctggcgtgt tcacgtcctg gtaactgaag cagatgctcg
     3841 cgcagggcat caattccccg accttcggtt gctgcggtga taaacagttt tgcctcagca
     3901 aaaccgtatt cccgcagcac ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg
     3961 tccacgcgat cggccttggt cagcgccact gtcagcatcg ggttaccggt cagctgcaaa
     4021 atcgccagat gctcacgggt ctgcgccatc acgccgtcat cgcacgccac caccaacagc
     4081 gcgtgatcga taccaccaac gcccgccagc atgttggaaa gaaacttttc atgaccgggt
     4141 acgtcgataa aaccaggcac gcgaccatcc ggctgcggcc agtaggcata gccgagatcg
     4201 atggtcatgc cgcgcttttt ttcttccggc agacggtcag catttacgcc agtaatcgcc
     4261 tgcaataagg ttgttttgcc gtggtcaacg tgtccggcag tcgcaataat catttcaaca
     4321 acatctccaa aaaccgttgc tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt
     4381 cataaatacg accaatcacc ggcactggca attcacgcca gcgggcggct aatgactcaa
     4441 ggtggctacc gcgtccatca tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa
     4501 ccggcagcga accactgcca atctgcgaaa gacatggcat aacctgtacc gcaaactccg
     4561 cgccgtaatg tgcggcaagg ggagcctgta aacgttgtgc ctggatttga atgacctctg
     4621 cgctgcgggt aagcaggcgc agggtcggta atttttcact cagagcttca gggtgtaaat
     4681 aaagacgcaa cgtggcttcc agcgccgcga gggtcatttt atccgcgcgt aatgcacgct
     4741 tcagcgggtg gctttgcagg cgggcgatca tctctttttt accaacgata atccctgcct
     4801 gcggcccgcc taacaacttg tcgccggaga agctcaccag actgacgccc gccgcaatca
     4861 actcctgcgg cattggctct ttcggcaaac cgtactggct aagatcgacc agcgagccac
     4921 tgcctaaatc agtcactacg ggaacatcca gctctttgcc gagcgccacc agttccgctt
     4981 catctatcgc tttggtgaac ccctgaatgc tgtagttact ggtatgtact ttcatcaaca
     5041 gtgcggtatt ttcattcacc gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc
     5101 ctacttcgtg tagggtgcag cctgcctgac gcataacatc gggaatacga aacgcgccgc
     5161 caatctccac caattcgccg cgagatacta ccacctcttt cccgctggca gtggccgcca
     5221 acatcaataa caccgccgcc gcattgttat tgacgataca ggcatcttcc gcccccgtaa
     5281 tacggcacag cagttgcgcc agcgcccgat cgcgatgtcc gcgtccagcg tcgtccagat
     5341 cgtactcgag ggtcactggc gaacgcatag cctgcgcaac ggcttccacc gcggcttccg
     5401 cctgtaaagc tcgtccaagg ttggtatgca gcacggttcc cgtcaggttg atcaccggac
     5461 gcagcgcgct ctgcgcctct ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac
     5521 accacgcagg aagcgtctgg ctgtcacgaa tcacttctcg cgcttcgtcg agcatctgac
     5581 gcaacaattc caccacgcgg gtgtgaccat aagtatcacg caaagaaagg aaggagctat
     5641 cgcgcaataa gcgatcaata gccggaagtt gactatagag ggaacgcgtt tcggttgtca
     5701 taggaaacct ggctaatcaa ggccctctca cacggagaag ggcgtttaac ataaccacgg
     5761 attgtaacgt gagatgggtc aggaggacat atcgcgcatc aagcctttgg cggttcggtg
     5821 cgagcaaaac tttcgcggct aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg
     5881 acacaccaac ctggcgcaac gcggcggaaa ttgagatatc cgacagcaca ggcaatggcg
     5941 atagtcgcca gattgaccgt atcggttttg agtgtgccat cgaccagata tccttccagc
     6001 acatccagac tgcggttgat tttctcccgc tggcgtaaca attcatcttc agactgctgc
     6061 gccgccggac gcgcctgttc acgcaccgat accagcccgg catccataat gccatccgcc
     6121 agcgcctcaa ttttgcgcac ccgcaacgac tccagcggat cgcgcggcaa catcgccgga
     6181 gcgacattca ttaactcaat atattcagcg atgatcggcg aatcaaacca gcattcgccc
     6241 tcttcggtca ccagcaccgg cacttttcct aacgggttaa attgcgccac gccgttgtcc
     6301 gcgttatagg gcagttcatt aataaattcg aaagttatgc ccttttctaa caacagaata
     6361 gaaagtttgc gtacaaacgg gctggtgtag ctaccgacga gtttcatgcc gagtcctttg
     6421 tgcgaggaaa aatatcagta tggcatggtg atgggaaaaa gggcagaaaa tgttgatggt
     6481 ttgttacttc caaataaatc acatatttat catggtgata taaatatttt cctaattatt
     6541 tcactctgat ggatatctca cttcaggctt tcttataaat ctgtagggtt ttgcctgtca
     6601 gcagacaaat aacccgataa aacaaggatg agaaatgagc ggaaaaccgg cagcgcgtca
     6661 gggcgacatg acgcagtatg gcggtagcat tgttcagggt tcagccgggg tgcgcattgg
     6721 tgcccccacc ggcgtggcct gttcggtgtg ccccggcgga gtgacgtccg gccatccggt
     6781 caatcccctg ctcggtgcaa aggtccttcc cggtgaaacc gacatcgccc tgcccggccc
     6841 gctgccgttc atcctctccc gcacctacag cagttaccgg acaaaaacgc ccgcgccggt
     6901 ggggagcctc ggccccggct ggaaaatgcc tgcggatatc cgcttacagc tgcgcgataa
     6961 cacactgata ctcagtgata acggcggcag aagcctgtat tttgagcacc tgtatcccgg
     7021 tgaggacggt tacagccgca gcgagtcact gtggctggtg cgcggcggcg tggcgaaact
     7081 ggatgaaggt caccggctgg ccgcactctg gcaggcgctg ccggaagaac tccgcttaag
     7141 tccgcatcgt tatctggcga caaacagtcc gcaggggccg tggtggctgc tcggctggtg
     7201 tgagcgggtg ccggaagcgg atgaggtgct gcctgcgccg ctgccgccgt accgggtact
     7261 gaccgggctg gtggaccgct tcgggcgcac acagacgttc caccgcgaag ccgccggtga
     7321 attcagcggc gaaatcaccg gcgtgacgga tggtgccggg cgtcacttcc aactggtact
     7381 gaccacgcag gcgcagcggg cagaagaagc ccggcagcag gccatttccg gcgggacgga
     7441 accgtccgct tttcctgata ccctgccggg ttacaccgaa tatggccggg acaacggcat
     7501 ccgtctgtct gccgtgtggc tgacgcacga cccgaaatac ccggagaatt tacctgccgc
     7561 gccgctggtg cgctatggct ggacgccgcg cggcgaactg gcggcagtgt atgaccgcag
     7621 caatacacag gtgcgtagct ttacttacga tgataaatac cggggccgga tggtggcgca
     7681 ccgtcacacg ggccggccgg aaatccgtta ccgttacgac agcgacgggc gggtgacaga
     7741 acagcttaac ccggcaggct taagctacac gtatcagtat gagaaggacc gcatcaccat
     7801 caccgacagc ctgaaccgcc gtgaagtgct gcacacgcag ggtgaagccg ggctgaagcg
     7861 ggtggtgaaa aaggaacacg cggacggcag cgtcacgcag agtcagtttg acgccgtggg
     7921 caggctcagg acacagacgg atgccgcagg ccggacaaca gaatacagcc cggatgtggt
     7981 gacgggcctc atcacgcgca tcaccacgcc ggatggcagg gcatcggcgt tttactatac
     8041 agccagttaa cgtcagccac cgggcctgac gggctggaaa tacgccggga atatgatgaa
     8101 tggggccgtc tgattcagga aactgcccct gacggcgata tcatccgcta ccgttatgat
     8161 aatccacaca gtgacttacc ctgcgcaacg gaagatgcca ccggtagccg gaaaaccatg
     8221 acgtggagcc gttacggtca gttgctgagc ttcaccgact gttccggtta tgtaacccgt
     8281 tatgaccatg accgcttcgg gcagatgacg gcggtgcacc gcgaggaagg gctgagtcag
     8341 taccgcgcat acgacagccg tggacagtta attgccgtga aagacacgca gggccatgaa
     8401 acgcggtatg aatacaacgc cgccggtgac ctgaccgccg tcattgcccc ggacggcagc
     8461 agaaacggga cacagtacga tgcgtgggga aaggccatcc gtaccacgca gggcgagctg
     8521 acgcgcagta tggaatacga tgctgcagga cgggtcatcc gcctgaccag tgaaaacggc
     8581 agccacacca ccttccgtta cgatgtactc gaccggctga tacaggaaac cggctttgac
     8641 ggccgcacac agcgttatca ccacgacctg accggcaaac ttatccgcag cgaggatgag
     8701 gggctggtca cccactggca ctatgacgaa gcagaccgcc tcacgcaccg caccgtgaat
     8761 ggcgaaaccg cagagcagtg gcagtatgac aaacgtggct ggctgacaga catcagccat
     8821 ctcagcgaag ggcaccgggt gacggtgcat tacaggtatg atgagaaagg ccggctgacc
     8881 ggtgagcgcc agacggtgca tcacccgcag ccggaagcac tgctctggca gcatgagacc
     8941 agacacgctt acaacgcgca gggactggcg aaccgctgta taccggacag cctgcccgcc
     9001 gtggaatggc tggcctacgg cagcggttac ctggcaggca tgaagctcgg cgacacaccg
     9061 ctggtggatt tcacccgcga ccgcctgcac cgggaaacgc tgcgccgctt cggccgctat
     9121 gaactcacca ccgcttatac ccctgccggg cagttacaga gccagcacct gaacagcctg
     9181 cagtatgacc gcgattacac ctggaacgac aacggcgaac tcatccgcat cagcagcccg
     9241 cgccagaccc ggagttacag ctacagcacc accggcaggc tgacggagaa aaccgacctc
     9301 atcccggaag ggggtatccg tacggatgat gagcgcaccc accggtacca ttacgacagt
     9361 cagcaccggc tggtgcacta cacgcggaca caatatgcag agccgctggt cgaaagtcgc
     9421 tatctttacg acccgctggg ccgcagggtg gcaaaacggg tgtggcggcg tgaacgtgac
     9481 ctgacgggct ggatgtcgct gtcacggaaa ccggaagtga cctggtacgg ctgggacggc
     9541 gaccggctga ccacaataca gaacgacagg agccgcatcc agacgattta tcagccgggg
     9601 agcttcacgc cactcatcag agttgaaacc gccaccggtg agctggcgaa aacgcagcgc
     9661 cgcagcctgg cggataccct tcagcagtcc ggcggcgaag acggtggcag tgtggtgttc
     9721 ccgccggtgc tggtgcagat gctcgaccgg ctggaaagtg aaatcctggc tgaccgggtg
     9781 agtgaggaaa gccgccgctg gctggcatcg tgcggcctga cggtggcgca gatgcaaagc
     9841 cagatggacc cggtgtacac gccggcgcga aaaatccacc tgtaccactg cgaccatcgc
     9901 ggcctgccgc tggcgctcat cagcacggaa ggggcaacag cgtggtgcgc agaatatgat
     9961 gaatggggca acctgctgag tgatgagaac ccgcatcatc tgcagcagct cattcgcctg
    10021 ccgggtcagc agtatgatga ggagtccggc ctgtattaca accgccaccg ctattatgac
    10081 ccgctgcagg ggcgatatat cactcaggac ccaattgggc tgaagggggg atggaatttt
    10141 tatcagtatc cgttgaatcc gatctcagag atagacccac aagggttaaa tccgttaatt
    10201 cttggtgttg tagcactgat gagttgcggt ttgttttcat caggtggtgt tatccaaagt
    10261 gggcaacaac gcagagatca atatggacgt aaagttaatc cagaagccga caaattaaca
    10321 gatgaacata agtcaggtgt aaatcctgga ggtaactgta gtccccagaa cctgagtgac
    10381 ttacaaaatg aaaaaaacag attatgtaac cagtctagag cttgcgctcc tcagatgctg
    10441 agaaaagaaa tattgagacg gtacgagata aatttggcgt gcgcttcggt cagaaagcag
    10501 attaataatc agtgctttgg tggtggagat aaggcccata tggaagaaga gaataaagca
    10561 tataaaacag cagctgattg cagtggctta ataaaatgaa aaaaataatt ttctctaacg
    10621 agagtgcagt ctatgatgaa cttatgattc atttcccatg ccagccatta cctcatattt
    10681 caaatgatat tatagggctg gaggaactgg atattgtata taattttttt ccaaaagaaa
    10741 caatggaatg aaataatcaa taatttaaaa ataaaggatg actcttatgc tttagagtta
    10801 ggaattatat tcctccctga gaaagtattc tgctattaca tccctctgta tatttatgtc
    10861 tcgttgttta ataagaatga tttttgggtt tttgaatccg attttattca acaatattta
    10921 tgtcctgagt atcgggatta cgatgatttt ttgatttttg tttttaattt ttcagatata
    10981 caactatcta ttattgcaca gtttatgtct tatgaatcag acgctggatt cttttatgca
    11041 agtaaagctt gtatggattt ttgggaagat tattcacctc tattacataa gaaaatttaa
    11101 tcgtaaaagg tataaatggt ttaataaaaa acttgtctac tcgttggagt agatgaaaaa
    11161 taagatgacg ttggcatata gatcactgcg aataatccac ctctaccact gcgactatcg
    11221 cggcctgccg ctgacgctta tcagccctga cggggcaaca gaatggtgcg cagaatatga
    11281 tgaatggggc aacctgctga atgaagagaa cccgcagcac ctgcagcagc ttatccgtct
    11341 gccggggcag cagtatgatg aggagtccgg cctgtattac aaccgcca
//
LOCUS       DAJTNA010000129        11119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTNA010000129 DAJTNA010000000
VERSION     DAJTNA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.130"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            121..987
                     /gene="folD"
                     /locus_tag="OO161_002924"
     CDS             121..987
                     /gene="folD"
                     /locus_tag="OO161_002924"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5463916.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            989..1201
                     /gene="ybcJ"
                     /locus_tag="OO161_002925"
     CDS             989..1201
                     /gene="ybcJ"
                     /locus_tag="OO161_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5463917.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1309..1830
                     /locus_tag="OO161_002926"
     CDS             1309..1830
                     /locus_tag="OO161_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5463918.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO161_002927"
     CDS             complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO161_002927"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5463919.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3425..3919
                     /gene="ppiB"
                     /locus_tag="OO161_002928"
     CDS             3425..3919
                     /gene="ppiB"
                     /locus_tag="OO161_002928"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5463920.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3922..4644
                     /gene="lpxH"
                     /locus_tag="OO161_002929"
     CDS             3922..4644
                     /gene="lpxH"
                     /locus_tag="OO161_002929"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5463921.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4762..5271
                     /gene="purE"
                     /locus_tag="OO161_002930"
     CDS             4762..5271
                     /gene="purE"
                     /locus_tag="OO161_002930"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5463922.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5268..6335
                     /gene="purK"
                     /locus_tag="OO161_002931"
     CDS             5268..6335
                     /gene="purK"
                     /locus_tag="OO161_002931"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5463923.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6472..7365)
                     /locus_tag="OO161_002932"
     CDS             complement(6472..7365)
                     /locus_tag="OO161_002932"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5463924.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQIPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLATALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7362..8177)
                     /locus_tag="OO161_002933"
     CDS             complement(7362..8177)
                     /locus_tag="OO161_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR5463925.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8188..9447)
                     /locus_tag="OO161_002934"
     CDS             complement(8188..9447)
                     /locus_tag="OO161_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR5463926.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSACMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMVRGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLDERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKKAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9457..11119)
                     /locus_tag="OO161_002935"
                     /pseudo
     CDS             complement(9457..11119)
                     /locus_tag="OO161_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2446 a         3179 c         2960 g         2534 t
ORIGIN      
        1 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
       61 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      121 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      181 caaaaagttc aggcgcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      241 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      301 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      361 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      421 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      481 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      541 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctc
      601 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      661 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      721 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      781 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
      841 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
      901 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgcttca ggcgtgcgtt
      961 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     1021 cgttgagctg tgcgatttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1081 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1141 caaaatcgtt gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1201 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1261 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1321 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1381 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1441 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1501 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1561 gggctgattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1621 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1681 ttcgctccct ggcaggtgat taaagtcgct ccgtttgcat tgtctcgtta caccacgccg
     1741 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1801 atgggaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     1861 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     1921 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     1981 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2041 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2101 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2161 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2221 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2281 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgtct gcttcaggtt ctcttcgctg
     2341 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2401 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2461 cggtcaacca tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca
     2521 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2581 tggatatcaa agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag
     2641 ccaggacgac ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2701 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     2761 tgcagctggt cgagatcctg acgcgacagc acgccataag tcggatcggt cgggacgtcg
     2821 aacatcacgt cgccgttgtc agccacataa gcgtgacctt tggcgatcag ttgttcagtg
     2881 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     2941 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3001 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3061 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3121 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     3181 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3241 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3301 catattttcc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3361 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     3421 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgacgataa
     3481 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3541 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3601 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac
     3661 ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt
     3721 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3781 ctactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     3841 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     3901 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     3961 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc tcgcaaggcc
     4021 gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga cgatcccaat
     4081 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4141 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagtggc
     4201 atgacgttat tgccggaaga aaaggtgctc gaactttatg gccgccgggt gttgattatg
     4261 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4321 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     4381 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     4441 gttaaccaaa acgcagtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4501 gggcataccc atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc
     4561 gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4621 gagctgattc attttccgtt ttaaaaaaac cacaactttg ctgatttcac agccacgcaa
     4681 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4741 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4801 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     4861 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     4921 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     4981 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5041 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5101 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5161 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5221 gcccagaccg acgaagtgct ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt
     5281 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5341 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5401 tgattaccgc tgagatcgaa cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc
     5461 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5521 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5581 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5641 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5701 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5761 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     5821 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     5881 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatgtgggc gtgatggcga
     5941 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6001 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6061 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6121 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6181 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6241 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6301 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6361 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6421 cgtcaagcgt cgcatcaggc atggtgcacc gattgccgga aactctcatc actacagcga
     6481 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6541 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6601 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6661 ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6721 ggcattaatc tgctcggcga gcaacgcagt ggcgagatct ttatcaatca ccgcttcact
     6781 ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     6841 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     6901 caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag
     6961 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7021 cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gtatctgcgg
     7081 ctgtgcgctc aagctctgcg ccagcatata gccaatcatc ccctggcttt ccgccaccag
     7141 cacatccagc ggatacggtt ctacctcttt ccacgccaga ttctgtaacg ccagcagccc
     7201 cacctgcggc ccgttgccgt gaacaatcgc cagccgataa gaacgggcca ggcgtgccag
     7261 cgcgggtaca gcactggcga tattgcgata ttgattttcc gccgtcagcg cctcaccgcg
     7321 ctggagtaag gcgttgcccc caagagctac aaccagtgtt ttcatggttt tccttgtaat
     7381 aattgttggc caagccagaa ccccagcagc gtatctgccc ctgacgtatg ccccagcgcc
     7441 agcagcgaat caatcgcaac agcggtacgt ttcgggcaac tcagagcatg gacaaagtgc
     7501 aggagtggcg aggcgaaata tccttgcgcg gcataacgta aataactgac gctcaccgct
     7561 gtggtaacga gttgaagatt gtcggaacag gcaaaaaacg ggcgaccggc gcgcgcatct
     7621 aaagcgccat aataccaagc cgccagcagc attccgctca acgtgtcatc atgacttggc
     7681 gttaatccgg ggcctttacc cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg
     7741 gcctgaaaac agtgacgaaa ctggcgtaat tcagcggtca gcggatcgct tgccgccatc
     7801 gccagtggtc cgaaaagtcc ggtttcctcc gcgcgttgca tccatgcagc tgcaagcggt
     7861 tgaggatgcg aaggcggcgt aatacgcagc aaacaataac gctgtggctg tttaaccgtg
     7921 aaacgcccga ggcgaatccc ttgagccaca acctgtggtc gttcattgcc gcataatcca
     7981 ccgcataacg catcgaattg cgcgcggcga agcacccatc ctccggggcc gaaaccacta
     8041 ccctgacgat gcagcgttaa cagttcgccg ctttccgtca tcaggttaat cgcccgccgc
     8101 cacactcccg ctaaccgcca ggactggcga taattcggtg cgctactgct ggcaagcaga
     8161 ggatggatga tcgtcattgc gcgttcctca accaatcccc atgctttcag ccagtgcttc
     8221 cagcgcctgt tcaaagcacg ccagcggtgc ccgcacggtg cctgcgccaa tctgcccgat
     8281 ccccgccttt ttatgggcga taccggtatt gatgagtggc gtaatgccgg tttctaccac
     8341 gcgacgaata tccagcccca ggcacgcgcc ctgaaaatcc cagcctggga tctgcaactg
     8401 catattgcgt tcaaggtaaa tttccgccat ctcttcagat accgccctgg ccgcttccat
     8461 gccacccgca ccgacaaagc gcgttacgcc aggcgctgcg atcattgccg cacctccgat
     8521 accaaaggtt tcggtaatcg cgctatcgcc catatccggg ttcgcctgct cctgcgagaa
     8581 gccggtgaaa aacagacctt gcggagtgtt tacaggcgca gtaaaccagc gttcatccag
     8641 cccgcttacc cgaatcccga acatattgcc gttgcgggtc atagccgtaa cgatgctgcc
     8701 tgcgcggatc atcgcgccag catccatcgc cgccttgcag taggccatcg cgaggttgag
     8761 gaagaactga tcggtcacgc tgaggaaatc gatcacttcc gcaatgtgct gtttatcatg
     8821 atcgaggcga gcaatttgtg gggccagcgt acgcattaac agtgctgagg aagcaatatt
     8881 gcgttgatgg aactcatcgc ccatcgtaat gccctgcgcc atcatcgccg tgagatcgat
     8941 accgcgcacc atgcgcccca gcgccgcgct taatactggc attaacacat cgcgcatcca
     9001 gcgatggcga gtcaggacat cttcgccgta agcgccaaaa cgcatcactt tgccgatacc
     9061 ttcgttgagg ttgcagtacg cccggttgcc gtcggttacg ttctcgacca ccagcatcgg
     9121 catacaggca gaagtaatgc cgcccattgg cccgacggca ttcacatggt gacaaggaat
     9181 gaagttcact tccccctgct ccagcatcgc cagtgcctgc gcttcatctt tcgcccagcc
     9241 ttcgaacaga catgcgccca cgcacgcccc tttcatgggt ccggtcatct cctgccagcg
     9301 cattggcggc ccggcgtgaa gcagtgtttt gccctcgttt agttcgctga taagtgaaaa
     9361 agccggttgc acatccagcc agtgtggacg agcacgacga atttgttcga taaccgcagc
     9421 attggcttgc gccactgatg taaacatggg aaccccttat tgcaaacgtt ctaataaacg
     9481 agccagtttt ttattgccgc cggcgactgg cgaccattgg taatgcacaa ccggtttgct
     9541 ggcgctttgt agctccagcg caaagctgcg taatccgatg ttaatcacgg cgacgttttc
     9601 cagtaatgac ggtgtatgtt gctgtgcggc aggcgagagc ggataaatta acgctgccgc
     9661 cagcaaggtg gcttccggca gcgaactcac taccgcgatc cccgcatctt ccagcgtggc
     9721 gatttgctgc gagcggcatt gcgggtcacg ttcagtgcct gtcaccgtgg caatggcata
     9781 cagtggttga ttatctgaac gcgcggcaca ggctttttgc taggcgctta ccagcgaggc
     9841 ggcaggatcg gcagtcgcac cgaagccaat cacgacatca agtagcaata cgcgcacttg
     9901 tggtttagcg ccgagatcgg caattaactg gttgcgtaag gctgggtcga tcatcggatg
     9961 gggacgcccg acggtgtaga aatcatcgcc gaggtcaata atctggtgac catcggcgtc
    10021 gagcatcatg ccgtgatgat gggtatcgtc ggcttccacg ccaaggtgtc cagcaagtaa
    10081 tcccgccgct tcggcagcca gcgtaccgcc ggtatacaaa ccgcaaataa atccgctgct
    10141 gacaggcgct attgcgttac gtcgcgccgt gacgcgtgaa agcaggcaag ccaggcgtgc
    10201 ggcctcatcc agcgaggagg caaaccagac attctcgtcg cgggccaccg ccggggtata
    10261 acctaaaaac agcgccaccg tcggtttgcc ggttgctttc atggcattaa caattttcag
    10321 acgcacagct tcggcaggtg gctttgaaac aaatgccagc acttcgcttt tctcgtctgc
    10381 actgagcatt tccagcgccg tcagcgcact gatgccgcct gcttcacggc tgaggtcgcg
    10441 ctcgccaaga ccaatcgcgt gagtaattcc ctcccctgcc tgcgcaatct gcgaacacag
    10501 ttcctgaatc ccggtaccgg aagcgccaat gacgccaatg ttgccttccg gcatcacgtt
    10561 agcaaaagcc agcggcgtgc cggcaatcat cgacgtaccg cagtccggcc ccatcaccag
    10621 caagcctttt tctcgcgcgc gggttttaag ttggatttca tcttccagcg tgacgttatc
    10681 ggagaacatc atcacgttga ggttgcgatc cagcgcctga ttcgccagct ccgccgcata
    10741 ctcgccagcc actgaaatca gcgccagatt ggcatcggat aatttctgac aggcactgtc
    10801 ccagcgacgc acctgcgtca aggactggct gctgcctgcc ccctgcgcca gttgttttag
    10861 cgcctcttca agctgctgca taatcgcctg cgcgatcccc gcatccgccg cttcgctacg
    10921 aattgccacg caaatatcgt tcggcgtggc gttattaaaa tcgtcatgcc agaaacctgt
    10981 ggtatctaat aacgctttat tggcgggcgt acccatcatt acggaaacat catcaacatt
    11041 ttctgattcg ctgagttttc gtgaaataat cattaaactg accgaatcct gaaaacaccc
    11101 ttttttaata aaggcgtgg
//
LOCUS       DAJTNA010000130        11108 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTNA010000130 DAJTNA010000000
VERSION     DAJTNA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.131"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..548
                     /gene="hdhA"
                     /locus_tag="OO161_002936"
     CDS             <1..548
                     /gene="hdhA"
                     /locus_tag="OO161_002936"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5463927.1"
                     /translation="LSALADFAISKLGKVDILVNNGGGGGPKPFDMPMADFRRAYELN
                     VFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHLVRNMAFD
                     LGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANAALFLCSP
                     AASWVSGQILTVSGGGVQELN"
     gene            777..1367
                     /gene="uidR"
                     /locus_tag="OO161_002937"
     CDS             777..1367
                     /gene="uidR"
                     /locus_tag="OO161_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5463928.1"
                     /translation="MMDNMQTEAQPTRPRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1755..3566
                     /gene="uidA"
                     /locus_tag="OO161_002938"
     CDS             1755..3566
                     /gene="uidA"
                     /locus_tag="OO161_002938"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5463929.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWVGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNAWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLEWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3563..4936
                     /gene="uidB"
                     /locus_tag="OO161_002939"
     CDS             3563..4936
                     /gene="uidB"
                     /locus_tag="OO161_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR5463930.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNIFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            4975..6240
                     /gene="uidC"
                     /locus_tag="OO161_002940"
     CDS             4975..6240
                     /gene="uidC"
                     /locus_tag="OO161_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5463931.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRIGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSSGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(6466..7974)
                     /locus_tag="OO161_002941"
     CDS             complement(6466..7974)
                     /locus_tag="OO161_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5463932.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLNGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8075..9250)
                     /gene="manA"
                     /locus_tag="OO161_002942"
     CDS             complement(8075..9250)
                     /gene="manA"
                     /locus_tag="OO161_002942"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5463933.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTHFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9449..11095
                     /gene="fumB"
                     /locus_tag="OO161_002943"
     CDS             9449..11095
                     /gene="fumB"
                     /locus_tag="OO161_002943"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5463934.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2769 a         2602 c         2867 g         2870 t
ORIGIN      
        1 aactctctgc actggcagac tttgctatca gtaagctggg taaagttgat attctggtta
       61 acaacggcgg tggcggtgga cctaaaccgt ttgatatgcc aatggcggat tttcgccgtg
      121 cttatgaact gaatgtgttt tcttttttcc atctgtcaca acttgttgcg ccagaaatgg
      181 aaaaaaatgg cggtggcgtt attctgacca tcacttctat ggcggcagaa aataaaaata
      241 taaacatgac ttcctatgca tcatctaaag ctgcggccag tcatctggtc agaaatatgg
      301 catttgacct gggtgaaaaa aatattcggg tgaatggcat tgcgccgggg gcaatattaa
      361 ccgatgccct gaaatccgtt attacaccag aaattgaaca aaaaatgtta cagcacacgc
      421 cgatcagacg tctgggtcaa ccgcaagata ttgctaacgc ggcgctgttc ctttgctcgc
      481 ctgcagccag ctgggtaagc ggacaaattc tcaccgtctc cggtggtggg gtacaggagc
      541 tcaattaata cactaacgaa ccggtaaaca gccgtatgtg ttgtttaccg ggatagcgtg
      601 tgcaattcat caacgtctct gctaaacaac tggcagccaa atcacggcta ttggttaacc
      661 aatagctgga tgaaaagcat acgaatagag tgtgccttcg cactattcaa cagcaatgat
      721 aggcgctcac ctgacaacgc ggtaaactag ttattcacgc taactataat ggtttaatga
      781 tggataacat gcagactgaa gcacaaccga cacggccccg gatcctcaat gctgccagag
      841 agattttttc agaaaatgga tttcacagtg cctcgatgaa agccatctgt aaatcctgcg
      901 ccattagtcc cgggacgctt tatcaccatt tcatctccaa agaagccttg attcaggcga
      961 ttatcttaca ggaccaggag agggcgctgg cccgtttccg ggaaccgatt gaagggattc
     1021 attttgttga ctatatggtc gagtctattg tctctctcac ccatgaagcc tttgggcaac
     1081 gggcgctggt ggtcgaaatt atggcggaag ggatgcgtaa cccacaggtc gccgccatgc
     1141 ttaaaaataa gcatatgacg atcactgaat ttgttgccca gcggatgcgt gatgcccagc
     1201 aaaaaggcga gataagccca gacatcaaca cgtcaatgac ttcacgttta ctgctggatc
     1261 tgacctacgg tgtactggcc gatatcgaag cggaagatct ggcgcgcgaa gcgtcgtttg
     1321 ctcagggatt acgcgcgatg attggcggta tcttaaccgc atcctgatcc tctcatttcg
     1381 cgggctggtg ataactgtgc ccgcgtttca tactttaatt tccctgtaca aaatacaatc
     1441 tagccgggat cggagaattc cccccaaaaa tattcaccgt agccatatgt catgagagtt
     1501 tatcgttctc aagatgcccg aacgaacgtt cgattgctta ttttatggct tctgtcaacg
     1561 ctgttttaaa gattaatgcg atctatatca cgctgtgcgt attgcagatt ttggtttttt
     1621 gatcgcggtg tcagttcttt ttatttccat ttctcttcca tgggtttctc acagataact
     1681 gtgtgcaaca cagaattggt taaccagtca gattaaaggt tgaccagtat ttttatttga
     1741 atgaggagtc ccttatgtta cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg
     1801 acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag cgttggtggg
     1861 aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac gatcagttcg
     1921 ccgatgcaga tattcgtaat tatgtgggca acgtctggta tcagcgcgaa gtctttatac
     1981 cgaaaggttg ggtaggccag cgtatcgtgc tgcgtttcga tgcggtcact cattacggca
     2041 aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg ccatttgaag
     2101 ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca
     2161 acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgatgaa aacggcaaga
     2221 aaaagcagtc ttacttccat gatttcttta actatgccgg gatccatcgc agcgtaatgg
     2281 tctacaccac gccgaacgcc tgggtggacg atatcaccgt ggtgacgcat gttgcgcaag
     2341 actgtaacca cgcgtctgtt gactggcagg tggtagcaaa tggtgatgtc agcgttgaac
     2401 tgcgtgatgc ggatcagcag gtggttgcaa ctggacaagg taccagcggg actttgcaag
     2461 tggtgaatcc ccacctctgg caaccgggtg aaggttatct ctatgaactg tgcgtcacag
     2521 ccaaaagcca gacagagtgt gatatctacc cgctgcgcgt cggcatccgg tcagtggcag
     2581 tgaaggacga acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc
     2641 atgaagatgc ggatttgcga ggcaaaggat tcgataacgt gctgatggtg cacgatcacg
     2701 cattaatgga ctggattggg gccaactcct accgaacctc gcattaccct tacgctgaag
     2761 agatgctcga gtgggcagat gaacatggca tcgtggtgat tgatgaaact gcagctgtcg
     2821 gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca
     2881 gcgaagaggc tgtcaacggg gaaactcagc aggcgcactt acaggcgatt aaagagctga
     2941 tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata
     3001 cccgtccgca aggtgcacga gaatatttcg cgccactggc ggaagcaacg cgtaaactcg
     3061 acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca
     3121 tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat gtccaaagcg
     3181 gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg caggagaaac
     3241 tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg ctgcactcaa
     3301 tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg
     3361 tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga
     3421 cctcgcaagg catattgcgc gttggcggta acaagaaggg gatcttcacc cgcgaccgca
     3481 aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa
     3541 aaccgcagca gggaggcaaa caatgaatca acaactctcc tggcgcacca tcgtcggcta
     3601 cagcctcggt gacgtcgcca ataacttcgc cttcgcaatg ggggcgctct tcctgttgag
     3661 ttactacacc gacgtcgctg gcgtcggtgc cgctgcggcg ggcaccatgc tgttactggt
     3721 gcgggtattc gatgccttcg ccgacgtctt tgccggacga gtggtggaca gtgtgaatac
     3781 ccgctgggga aaattccgcc cgtttttact cttcggtact gcgccgttaa tgatcttcag
     3841 cgtgctggta ttctgggtac cgaccgactg gagccatagc agcaaagtag tgtatgcata
     3901 tttgacctac atgggcctcg ggctttgcta tagcctggtg aatattcctt atggttcact
     3961 tgctaccgcg atgacccaac aaccacaatc ccgcgcccgt ctgggcgcgg ctcgtgggat
     4021 tgccgcttca ttgacctttg tctgcctggc atttctgata gggccgagca ttaagaactc
     4081 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg cgattgccgg
     4141 aatggtgctt tacttcatct gcttcaaatc gacgcgtgag aatgtggtac gtatcgttgc
     4201 gcagccgtca ttgaagatca gtctgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     4261 gtgcatcggt gcgctgtgtg tgctgatttc gacctttgcg gtcagcgcct cgtcgttgtt
     4321 ctacgagcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     4381 cttggttggt actgtggcat cggcaccgct ggtgccgggg atggtcgcga ggagcggtaa
     4441 aaagaatatc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     4501 ggtttccgtc tggtcactac cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     4561 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     4621 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     4681 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     4741 caatcaggtg caaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     4801 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4861 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4921 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4981 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttat
     5041 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     5101 agggcggata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     5161 ttgctcgatt tcaatagtgg ttattattcc aatattattg gcgttgaagg cggggcatat
     5221 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     5281 aaaagttttg gctttgccct gggggcagta aaaataaaac cctgtgaaaa tagcctgctt
     5341 aaattaggtc gcatcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     5401 atggctggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     5461 gctttaacac caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     5521 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     5581 aaacatcgcg cacgttcttt tttagccgcc agttggcatg atgataccag tcgctattct
     5641 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     5701 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     5761 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     5821 acctggaatg cgccgtgggg aagcgtattt ggcagtagtg gttatttgcg ccatgcaatg
     5881 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5941 cgtgaaggaa tgcagtcctg gcaattgggc gtcaactatc gtttaacgcc gcaatttaca
     6001 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     6061 gaaggcacgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     6121 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     6181 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     6241 gaaataacgc cggagagaaa aacctcaggc gtttcagatt gttgacaaag tgcgcgtttt
     6301 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     6361 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     6421 gatgaaaacg ccgaagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     6481 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     6541 cgacattttc tgcccattta acgttatctg accgttagta tattgcaggc tggtggtgat
     6601 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     6661 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     6721 agtcataaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     6781 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6841 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6901 gacagtaatc accggatcgc cattcaacat cagcggcagg gcgctaaaga aggcttccgt
     6961 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     7021 agtttgcgcg ttatattgct ggctgaactg atgccaggct tcaccatcaa tctggccgac
     7081 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     7141 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     7201 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     7261 tgacagtttt tggtttccta cgcgctcacc aaaacttgcc agcgtgctgg aaccgtcggt
     7321 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     7381 accactttgc gcctccccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     7441 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggcttgag
     7501 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     7561 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     7621 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     7681 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     7741 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     7801 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7861 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7921 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7981 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     8041 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     8101 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     8161 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     8221 acaaaatggc ggcactctgc tggctaatgg tggtttcttt atcactaagg tcatgcagcg
     8281 agaaggcaaa atcatccact ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     8341 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     8401 tatcaatgta cttaggcgtc agacccgcac gcagcacgtt atcggagttc gccatcactt
     8461 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     8521 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     8581 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     8641 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggctggcga
     8701 acagttcgct taaacgttcg gcattaggct gttgtaaaaa gtgagcaatt gccggatgtg
     8761 cacctgcgac tggctggagt agggagacaa tctcggaaaa ctcacgaaac gcgttcatcg
     8821 caaggaaatg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8881 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8941 tgcgtttgtt tggatgaacc tgaatggaga gcggctgtgc tgcgcataat actttgaaca
     9001 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     9061 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     9121 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     9181 cataaagttc agtcaacgcc gttttgctgc cccaggcata gttttgcact gagttaatga
     9241 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccactatt aaagcaagaa
     9301 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     9361 agtgtgtagg atattgttac tcgcttttta cagggcaacg gaacacccgc ccagagcata
     9421 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcaggctcc
     9481 ttttccactc aaaaaagatg atactgagta ttacctgcta accagcgaac acgttagcgt
     9541 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     9601 gcgtcaggcg tttcatgatg cgtcatttat gctgcgtccg gctcaccaac aacaggtggc
     9661 cgacattctg cgtgacccgg aagccagcga aaatgataaa tatgtggcgc tgcaattcct
     9721 gcgcaactcc gacatcgcgg cgaaaggtgt tctgccaacc tgtcaggata ccggcactgc
     9781 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggctgtgatg aagcggcgct
     9841 ggcgcgcggt gtctataaca cttatatcga agataatttg cgctactcgc aaaacgcgcc
     9901 gctggatatg tataaagagg tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9961 tgccgttgat ggcgacgagt acaaattcct ctgtatcgcc aaaggtggtg gttcggcaaa
    10021 caagacgtat ctctatcagg aaaccaaagc gttactaacg ccggggaaac tgaaaaatta
    10081 cctggttgag aagatgcgca cgctgggtac ggcggcctgt cctccgtatc atattgcgtt
    10141 cgtaattggt ggaacttctg cagaaactaa ccttaaaaca gtgaaactgg cttccgctaa
    10201 atactatgac gaactgccaa cggaagggaa tgagcacggt caggcattcc gcgatgtgga
    10261 actggaaaaa gaattgctga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
    10321 taaatacttc gctcacgaca tacgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
    10381 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
    10441 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
    10501 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
    10561 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
    10621 cggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
    10681 gcagtacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
    10741 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
    10801 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10861 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10921 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10981 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    11041 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    11101 gccgccct
//
LOCUS       DAJTNA010000131        10950 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTNA010000131 DAJTNA010000000
VERSION     DAJTNA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10950
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.132"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(164..496)
                     /gene="emrE"
                     /locus_tag="OO161_002944"
     CDS             complement(164..496)
                     /gene="emrE"
                     /locus_tag="OO161_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5463935.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYYASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(830..1144)
                     /gene="fliE"
                     /locus_tag="OO161_002945"
     CDS             complement(830..1144)
                     /gene="fliE"
                     /locus_tag="OO161_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5463936.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1359..2528
                     /gene="fliF"
                     /locus_tag="OO161_002946"
     CDS             1359..2528
                     /gene="fliF"
                     /locus_tag="OO161_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR5463937.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEAILSPIVGNGNIHAQVTA
                     QLDFASKEQTEEQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNA
                     PISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAV
                     VVNYKTLPDGKPLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGEL
                     PFWQQQAFIDQLLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVE
                     DAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2521..3516
                     /gene="fliG"
                     /locus_tag="OO161_002947"
     CDS             2521..3516
                     /gene="fliG"
                     /locus_tag="OO161_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5463938.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAIANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3509..4195
                     /gene="fliH"
                     /locus_tag="OO161_002948"
     CDS             3509..4195
                     /gene="fliH"
                     /locus_tag="OO161_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5463939.1"
                     /translation="MSDNLPWKTWTPDDLAPPEAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATFSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4195..5482
                     /gene="fliI"
                     /locus_tag="OO161_002949"
                     /pseudo
     CDS             4195..5482
                     /gene="fliI"
                     /locus_tag="OO161_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     gene            5418..6572
                     /gene="fliK"
                     /locus_tag="OO161_002950"
     CDS             5418..6572
                     /gene="fliK"
                     /locus_tag="OO161_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5463940.1"
                     /translation="MPKAAIRCSTIRLAPLITADVDTTTLPGGKASDAAQDFLALLSE
                     ALAGETTTDKAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQS
                     TSTPLTTAQTMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVP
                     STVLPAEKPTLFTKLTSAQLTTAQPDDSPGTPAQPLTPLVAEAQSKAEVISTPSPVTA
                     AASPLITPHQTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQD
                     LGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESF
                     SGQQQAASQQQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            6677..7141
                     /gene="fliL"
                     /locus_tag="OO161_002951"
     CDS             6677..7141
                     /gene="fliL"
                     /locus_tag="OO161_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5463941.1"
                     /translation="MTDYSISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7146..8150
                     /gene="fliM"
                     /locus_tag="OO161_002952"
     CDS             7146..8150
                     /gene="fliM"
                     /locus_tag="OO161_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5463942.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKSLRGAGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8147..8560
                     /gene="fliN"
                     /locus_tag="OO161_002953"
     CDS             8147..8560
                     /gene="fliN"
                     /locus_tag="OO161_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5463943.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            8563..8928
                     /gene="fliO"
                     /locus_tag="OO161_002954"
     CDS             8563..8928
                     /gene="fliO"
                     /locus_tag="OO161_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5463944.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            8928..9590
                     /locus_tag="OO161_002955"
     CDS             8928..9590
                     /locus_tag="OO161_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5463945.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDNIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLAQSF
                     YS"
     gene            9600..9869
                     /gene="fliQ"
                     /locus_tag="OO161_002956"
     CDS             9600..9869
                     /gene="fliQ"
                     /locus_tag="OO161_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5463946.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            9878..10659
                     /gene="fliR"
                     /locus_tag="OO161_002957"
                     /pseudo
     CDS             9878..10659
                     /gene="fliR"
                     /locus_tag="OO161_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
BASE COUNT         2631 a         2888 c         2928 g         2503 t
ORIGIN      
        1 cctctttgtt gtgacctatc gttattttat ttgatgtgca ttgatctcat tatgcaaaaa
       61 ggcgcaatga tcgcgcaaat gcaatcataa ttataaagtt gccccgaaca ttagccataa
      121 tatgcacaaa tcagattcct gttatctcaa ggatgattat tttttaatgc ggtgcgcttc
      181 gtgacaacaa gttaatgact aacacaccgg cacaaatcaa catcatgcct atgatggctg
      241 gcaagtccag tcgttggcca aaaaatcccc aggaaagcaa actaatcaga acgataccga
      301 ctcctgacca gatagcataa gcaatccctg taggtatata agccagtgtc tgagctaata
      361 accaaaatga tgcataatag caaataattg tgccaacaga gggccataac cgtgtaaacc
      421 cctctgaaaa cttcattaag gttgtaccaa tgacctctgc aagtattgcg ccaccaagat
      481 aaatataagg gttcatagca tattctttcc tgttcatact ggagagaatt gtactacagt
      541 ttgaacacaa ctcaccgttt tcatcctggt gtatgtgtat ccagggggct ctggcgtggc
      601 aagagataaa ggcaggaaca ataatgatca gaaggaaccc caggctaaca aaagagttca
      661 ctcttcctat gagcaaagcg aagacaaaac atgttatcgc gtttacggat cgtggatggc
      721 agaagacttt caggatcagg tatccatttt aaaccagaaa tagggtgagt ttgtcccatc
      781 catgccccac gcgggcagat gggatgtaat taccaacagg ttacaaaacc tacacctgca
      841 tggacatcac ttcctgatac gccgccacca gcttattacg cacctgaatc cccatttgca
      901 tagaaactga ggctttttgc atatcggtca tcacatcgtt taacgccacg ccgggttcac
      961 cgagggtgaa tttttctgcc tgcgtgcggg cggctgtttg tgtatcgctt atgcgatcga
     1021 gcgcggcgtg cagctgcccg gcaaaactaa tggtcggttg cggcagtgaa tcctgcgcac
     1081 gcgcactcat cgccgtagcc tgtaactggc tgataacccc ttcaatcccc tgtatcgctg
     1141 acattttcgt ctcctggata atttctggta gcaaagccta ccagtaagtc aataagacaa
     1201 aggcgctaaa tagcaacaaa aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca
     1261 aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt gttccacttt
     1321 gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact gcagcccaga
     1381 caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg
     1441 ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact
     1501 accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga
     1561 cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgaa gcgatcctgt
     1621 cgccaattgt tggtaacggt aatattcacg cccaggtcac ggcgcaactg gacttcgcca
     1681 gtaaagaaca aacggaagaa cagtatcgcc ctaacggtga tgaatctcat gcggcgcttc
     1741 gttcacgcca gcttaatgag agcgagcaaa gcggttccgg ttatccgggc ggcgtaccgg
     1801 gggcgttgtc gaatcaaccg gcacctgcga ataacgcgcc aatcagcacg cctccggcaa
     1861 atcaaaataa ccgccagcag caggcgagca ccaccagcaa tagcgggccg cgtagcacac
     1921 agcggaatga aaccagtaac tacgaagtcg atcgcaccat tcgtcatacc aaaatgaacg
     1981 tgggcgatgt gcaacgtctg tcagtcgcgg tagtggtgaa ttacaaaacc ttgccagacg
     2041 gtaaaccatt gcctctcagc aacgaacaga tgaagcaaat tgaagatctg acccgcgagg
     2101 cgatgggctt ttctgaaaaa cgcggtgact cgctcaatgt cgttaactcg ccgttcaata
     2161 gcagtgacga aagcggcgga gaactgccgt tctggcaaca gcaagcgttt atcgatcagt
     2221 tgctggctgc cggtcgctgg ttgctggtac tgctggtggc gtggctgctg tggcggaaag
     2281 cggtacgccc gcagttaaca agtcgcgccg aggcgatgaa agctgtacag caacaggcgc
     2341 aggcccgtga ggaagtggaa gatgcggtgg aagtccgcct gagcaaagac gaacaactcc
     2401 aacaacgacg cgctaaccaa cgtctggggg cagaagtcat gagccagcgt atccgtgaaa
     2461 tgtctgataa cgatccgcgc gtggtggcgc tggtcattcg ccagtggata aataacgatc
     2521 atgagtaacc tgacaggcac cgataaaagc gtcatcctgc tgatgaccat tggcgaagac
     2581 cgggcggcag aggtgttcaa gcacctctcc cagcgcgaag tgcaaaccct gagcgctgca
     2641 attgcgaacg tcacgcagat ctccaacaag cagctaaccg atgtgctggc ggagtttgag
     2701 caagaagctg aacagtttgc cgcactgaat atcaacgcca acgattatct gcgttcggta
     2761 ttggtcaaag ctctgggtga ggaacgtgcc gccagcctgc tggaagatat tctcgaaact
     2821 cgcgataccg ccagcggtat tgaaacgctc aactttatgg agccgcagag tgccgccgat
     2881 ctgattcgcg atgagcatcc gcaaattatc gccaccattc tggtgcatct gaagcgcgcc
     2941 caggccgccg atattctggc gttgttcgat gaacgtctgc gccacgacgt gatgttgcgt
     3001 atcgccacct ttggcggcgt gcagccagcc gcgctggcgg aactgaccga agtactgaat
     3061 ggcttgctcg acggtcagaa tctcaagcgc agcaaaatgg gcggcgtgag aacggcagcc
     3121 gaaattatca acctgatgaa aactcagcag gaagaagccg ttattaccgc cgtgcgcgaa
     3181 ttcgacggcg agctggcgca aaaaattatc gacgagatgt tcctgttcga aaatctggtg
     3241 gatgtcgacg atcgcagcat tcagcgtctg ttgcaggaag tggattccga atcgctgttg
     3301 atcgcgctga aaggggccga gcagccactg cgcgagaaat tcctgcgcaa tatgtcgcag
     3361 cgtgccgccg atattctgcg cgacgatctc gccaaccgtg gtccggtgcg tctgtcgcag
     3421 gtggaaaacg aacagaaagc gattctgctg attgtgcgcc gccttgccga aactggcgag
     3481 atggtaattg gcagcggcga ggatacctat gtctgataat ctgccgtgga aaacctggac
     3541 gccggacgat ctcgcgccac cagaggcaga gtttgtgccc atggtcgagc cggaagaaac
     3601 catcattgaa gaggccgaac ccagccttga gcagcaactg gcgcaactgc aaatgcaggc
     3661 ccatgagcaa ggttatcagg cggggattgc cgaaggtcgc cagcaaggtc atgagcaggg
     3721 ctatcaggaa ggactggccc aggggctgga gcaaggtctg gcagaggcga agtctcaaca
     3781 agcgccaatt catgcccgga tgcagcaact ggtcagcgaa tttcaaacta cccttgatgc
     3841 gcttgatagt gtgattgctt cgcgcctgat gcagatggcg ctggaggcag cacgtcaggt
     3901 catcggtcag acgccaacag tggataactc ggcactgatc aaacagatcc aacagttgtt
     3961 gcagcaagaa ccgttattca gcggtaaacc acagctgcgc gtgcacccgg atgatctgca
     4021 acgtgtggat gatatgctcg gcgctacctt cagtttgcat ggctggcgct tgcggggcga
     4081 tcccaccctc catcctggcg gctgtaaagt ctccgccgat gaaggcgatc tcgacgccag
     4141 tgtcgccact cgctggcaag aactctgccg tctggcagca ccaggagtgg tgtaataacc
     4201 acgcgcctga ctcgctggct aaccacgctg gataactttg aagccaaaat ggcgcagttg
     4261 cctgcggtac gtcgctacgg gcgattaacc cgcgctaccg ggctggtgct ggaagccacc
     4321 ggattacaat tgccgctcgg cgcaacctgt gtgattgagc gccagaacgg cactgaaacg
     4381 cacgaagtag aaagtgaagt cgttggcttt aacggtcaac ggctgttttt aatgccgctg
     4441 gaggaagtcg aaggtgtcct gcccggcgcg cgtgtttatg ccaaaaacat ttcggcagaa
     4501 gggctgcaaa gcggcaaaca gttaccgctc ggtccggcgt tattaggtcg tgttctggac
     4561 ggcagcggta aaccgctcga tggcctcccc cgatacgacg gaaaccggtg cgctgattac
     4621 cccgccattt aacccgttgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg
     4681 cccaatcaat gccctgctta ccgttgggcg tgggcagcgt atggggctgt ttgccgggtc
     4741 cggcgttggt aaaagtgtgc tgctggggat gatggcacgt tacacccgcg ccgatgtcat
     4801 tgtcgtgggt ttgattggtg aacgcggacg cgaagtaaaa gattttattg agaatatcct
     4861 cggtgccgaa gggcgtgcac gctccgtggt gattgccgct ccggcggatg tttctcctct
     4921 cctgcgaatg cagggtgccg cctatgccac gcgcattgcc gaagattttc gcgatcgtgg
     4981 tcagcatgtg ttgctgatta tggactccct cacccgctac gcgatggccc agcgtgaaat
     5041 tgcgctggcg attggcgaac cacccgccac taaaggttat cccccgtcgg tgtttgccaa
     5101 attaccggca ctggtcgagc gtgccggaaa tggcattagc ggcggcggat cgattaccgc
     5161 gttttatacc gtgctcactg aaggcgatga ccagcaggac cccattgccg actctgcgcg
     5221 ggcaatcctc gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc
     5281 ggctatcgat attgaagcgt cgatcagccg cgcaatgacg gcgttgatca gtgagcaaca
     5341 ttacgcgcga gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta accgcgatct
     5401 ggttagcgtc ggcgcgtatg ccaaaggcag cgatccgatg ctcgacaatt cgcttagcgc
     5461 ccttaattac cgccgacgtt gacaccacca cattgcctgg cggcaaagcc agcgatgctg
     5521 cacaagattt tctcgcgttg ttgagcgaag cattagcagg cgagacaact accgacaaag
     5581 cggcccccca gttgctggtg gcaacagata agcccacgac aaaaggcgag ccgctgatca
     5641 gcgatattct tgccgacgcg caacaagcgg atttactgat ccccgtggat gaaacactgc
     5701 ctgtcatcaa cgacgaacaa tccacatcaa caccgttaac caccgctcag acgatgacgt
     5761 tggctgcggt ggctgaccaa aatacgacaa aagacgaaaa agcggatgat ctgaatgaag
     5821 acgtcaccgc aagcctgagc gccctttttg cgatgttgcc gggttttgac aatacgccca
     5881 aagtgactga tgtgccgtca accgtgttac cggcagagaa accaacgctc ttcacaaaac
     5941 tgacttctgc gcaactcaca acagcacagc ctgatgattc ccccggcacg ccagctcagc
     6001 cattaacacc gctggtagca gaagcccaga gtaaagcgga agtcatcagc acaccgtcac
     6061 cggtgaccgc tgccgccagc ccgctaatta ctccacacca gacacagcca ctgcccaccg
     6121 tcgccgcgcc tgttttgagt gcaccgctgg gttctcacga atggcaacaa tcattaagcc
     6181 agcatatttc gctgttcacc cgccaggggc aacaaagtgc agagttgcgt ctgcacccgc
     6241 aggatttagg tgaagtgcaa atctccctca aagtggatga taaccaggcg caaatccaga
     6301 tggtttcacc gcatcagcat gtacgcgccg cgctggaagc agcgcttccg gtactgcgta
     6361 cgcagctggc cgaaagtggc attcagttag ggcaaagcaa catcagtggc gaaagcttta
     6421 gtggtcagca gcaggccgct tcccaacaac agcaaagcca acgcacagta aaccatgaac
     6481 ctctggcggg ggaagacgac gatacgcttc cggttcccgt ctctttacaa gggcgtgtaa
     6541 caggcaacag cggcgttgat attttcgcct aacgtcagag gtagcaccgt aatccgcgtc
     6601 ttttccccgc tttgttgcgc tcaagacgca ggataattag ccgataagca gtagcgacac
     6661 aggaagaccg caacacatga ctgattactc gataagcaag aaaagcaagc gatcgctttg
     6721 gatcccgatt ctggtattca ttaccctcgc ggcctgtgcc agcgcaggtt acagctactg
     6781 gcattcgcat caggttgccg ctgacgacaa agcgcagcaa cgcgtcgtac cctccccggt
     6841 cttctacgcg ctggatacct tcacggtcaa tttgggcgat gcggatcgcg tgctttatat
     6901 cggcataacc ctgcgcctga aagatgaagc tacccgctcg cggctgagtg agtatttgcc
     6961 ggaagtccgt agccgtttgc tgttactgtt ttcgcgtcag gatgctgccg tactggcgac
     7021 agaagaaggc aagaaaaacc tgattgccga gattaaaacc acgctttcca ccccgcttgt
     7081 tgccgggcaa ccgaaacagg atgtcaccga cgtgctgtat accgctttta ttctgcgata
     7141 acgacatggg cgatagtatt ctttctcaag ctgaaattga tgcgctgttg aatggtgaca
     7201 gcgaagtcaa agacgaaccg acagccagtg ttagcggcga aagtgacatt cgtccgtacg
     7261 atccgaatac ccaacgacgg gttgtgcgcg aacgtttgca ggcgctggaa atcattaatg
     7321 agcgctttgc ccgccatttt cgtatggggc tgttcaacct gctgcgtcgt agcccggata
     7381 taaccgtcgg ggccatccgc attcagccgt accatgaatt tgcccgcaac ctgccggtgc
     7441 cgaccaacct gaaccttatc catctgaaat cgctgcgcgg cgcagggctg gtggtgtttt
     7501 caccgagtct ggtgtttatc gccgtggata acctgtttgg cggcgatgga cgcttcccga
     7561 ccaaagtgga aggtcgcgag tttacccata ccgaacagcg cgtcatcaac cgcatgttaa
     7621 agctggcgct tgaaggctat agcgacgcct ggaaggcgat taatccgctg gaagttgagt
     7681 acgtgcgttc ggaaatgcag gtgaaattta ccaatatcac cacctcgccg aacgacattg
     7741 tggttaacac gccgttccat gtggagattg gcaacctgac cggcgaattt aatatctgtc
     7801 tgccattcag catgatcgag ccgctacggg aattgttggt taacccgccg ctggaaaact
     7861 cgcgtaatga agatcagaac tggcgcgata acctggtgcg ccaggtgcag cattcacagc
     7921 tggagctggt cgccaacttt gccgatatct cgctacgcct gtcgcagatt ttaaaactga
     7981 aacccggcga cgtcctgccg atagaaaaac ctgatcgcat catcgcccat gttgacggcg
     8041 tcccggtgct gaccagtcag tacggcactc tcaacggtca gtatgcgtta cggatagaac
     8101 atttgattaa cccgatttta aattctctga acgaggaaca gcccaaatga gtgacatgaa
     8161 taatccggcc gatgacaaca acggcgcaat ggacgatctg tgggctgaag cgttgagcga
     8221 acaaaaatca accagcagca aaagcgctgc cgacgcggtg ttccagcaat ttggcggtgg
     8281 tgatgtcagc ggaacgttgc aggatatcga cctgataatg gatattccgg tcaagctgac
     8341 cgtcgagctg ggccgtacgc ggatgaccat caaagagctg ttgcgtctga cgcaagggtc
     8401 cgtcgtggcg ctggacggtc tggcgggcga accactggat attctgatca acggttattt
     8461 aatcgcccag ggcgaagtgg tggtcgttgc cgataaatat ggcgtgcgga tcaccgatat
     8521 cattactccg tctgagcgaa tgcgtcgcct gagccgttag tgatgaataa ccacgctact
     8581 gtgcaatctt ccacgccggt ttctgctgcg ccactgctgc aggtcagcgg cgcactcatc
     8641 gccattattg ccctgatcct cgctgctgcc tggcaggtaa aacggttggg atttgccccg
     8701 aaacgcactg gcgttaacgg tctgaaaatt agcgccagtg cttcgctggg cgcgcgtgaa
     8761 cgggttgtgg tggtcgatgt ggaagatgca cggctggtgc tcggcgttac cgcagggcaa
     8821 atcaatctgc tgcataaact tcccccttca gcaccaacag aagagatacc gcagaccgat
     8881 tttcagtcgg tcatgaaaaa tttgcttaag cgtagtggga gatcctgatg cgtcgtttgt
     8941 tgtctgtcgc acctgtcctt ctctggctgg ttacgcccct cgccttcgcg caactgccgg
     9001 gtatcaccag ccagccgctg cctggcggtg gacaaagctg gtcgctcccg gtgcagacgc
     9061 tggtgttcat cacctcgttg acgtttattc cggcaatttt actgatgatg accagtttca
     9121 cccgcatcat cattgttttt ggtttattgc gtaacgcgct gggaacgccc tccgcgccac
     9181 ctaaccaggt attgctgggg ctggcactat ttttgacctt ttttattatg tcaccggtga
     9241 tcgacaacat ttatgtcgat gcgtaccagc cattcagcga agagaaaata tcaatgcagg
     9301 aggcgctgga aaaaggggcg cagccgctac gtgagtttat gctgcgtcag acccgtgagg
     9361 cagatttagg gttgtttgcc agactagcga ataccggccc gttgcaggga cctgaagccg
     9421 tgccgatgcg cattttgctc ccggcctacg tgaccagcga gttgatggca ttggggatga
     9481 tgatggtccc ccccgccacc attgctctgc cctttaaact gatgctgttt gtactggtgg
     9541 atggctggca attgctggtc ggttcgctgg cgcagagctt ttacagctag agaggcaaaa
     9601 tgacacctga atcggtcatg atgatgggga ctgaagcgat gaaagtcgcg ctggcactgg
     9661 ctgccccgct gttgctggtg gcgctggtca cgggccttat catcagtatt ttgcaggccg
     9721 ccacgcagat caacgaaatg acgctgtcgt ttattccaaa aatcatcgcc gtatttattg
     9781 ccattattat tgccggaccg tggatgctca atctgttgct ggattacgtc cgcaccttgt
     9841 tcactaacct gccgtatatc atcgggtagg ccgtacaatg atgcaggtga caagcgatca
     9901 atggctttcc tggttaagcc tgtacttctg gccgttactg cgcgtgctgg cgctcatttc
     9961 taccgcacca attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg ggctggcaat
    10021 tatgatcacg ttcgtcattg ccccatcatt acctgccaac gatgttcctg ttttttcgtt
    10081 ctttgctctg tggctggccg tgcagcagat cctgatcggc attgcgcttg gttttaccat
    10141 gcaatttgcc tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct
    10201 gtcatttgcg acgtttgtcg atccggccag ccatctcaat atgcccgttt tagcgcgtat
    10261 catggatatg ctggcgttac tgctgttcct gacatttaac ggtcatttat ggttgatttc
    10321 actgctggtc gatacctttc acaccctgcc aattggtggc gaaccgttga acagcaatgc
    10381 gtttctggca ctcaccaaag cggggagttt gattttcctt aacgggctga tgctggcgtt
    10441 accgctcatt actctgctgc tgaccctgaa tctggcatta ggtttactga atcgtatggc
    10501 cccgcaatta tccatttttg ttattggatt tccattaact ctgactgtcg gtatctcttt
    10561 aatggcggca ttaatgccgt taattgcacc tttttgcgaa catttattca gtgagatgtt
    10621 tagctggctg atattattag tgaattgcca ttaatataat tccgtaacgt ttatcatgtt
    10681 atcctaagga ttatccgaaa aataatacct acgaacatct tccaggatac tcctgcggcg
    10741 aaatatttgt tttaagctta ctcacatatc gcaacattta ctttaagaca attccaggca
    10801 aattatacaa cactttacgg gatagtaagt ccgcctgaaa aatcgcgaga gtggcgcatt
    10861 aggtgaccca tgttgttccg ttagtcatga tgaaatattc aggtaagggg aattatcgtt
    10921 acgcattgag tgagggtatg ccatgtcaac
//
LOCUS       DAJTNA010000132        10905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTNA010000132 DAJTNA010000000
VERSION     DAJTNA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.133"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..1074
                     /locus_tag="OO161_002958"
     CDS             <1..1074
                     /locus_tag="OO161_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5463947.1"
                     /translation="QIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAV
                     IRYDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVP
                     TPFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMA
                     VFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLL
                     SCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKM
                     PPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEE
                     NQGSNLSGPFLPGQSRTFSYRLA"
     gene            1099..1326
                     /locus_tag="OO161_002959"
     CDS             1099..1326
                     /locus_tag="OO161_002959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5463948.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            1922..3490
                     /gene="cydA"
                     /locus_tag="OO161_002960"
     CDS             1922..3490
                     /gene="cydA"
                     /locus_tag="OO161_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5463949.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            3506..4645
                     /gene="cydB"
                     /locus_tag="OO161_002961"
     CDS             3506..4645
                     /gene="cydB"
                     /locus_tag="OO161_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5463950.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            4660..4773
                     /gene="cydX"
                     /locus_tag="OO161_002962"
     CDS             4660..4773
                     /gene="cydX"
                     /locus_tag="OO161_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5463951.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            4773..5066
                     /gene="ybgE"
                     /locus_tag="OO161_002963"
     CDS             4773..5066
                     /gene="ybgE"
                     /locus_tag="OO161_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5463952.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            5216..5620
                     /gene="ybgC"
                     /locus_tag="OO161_002964"
     CDS             5216..5620
                     /gene="ybgC"
                     /locus_tag="OO161_002964"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5463953.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERIAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            5617..6309
                     /gene="tolQ"
                     /locus_tag="OO161_002965"
     CDS             5617..6309
                     /gene="tolQ"
                     /locus_tag="OO161_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5463954.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            6313..6741
                     /gene="tolR"
                     /locus_tag="OO161_002966"
     CDS             6313..6741
                     /gene="tolR"
                     /locus_tag="OO161_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5463955.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            6806..7960
                     /gene="tolA"
                     /locus_tag="OO161_002967"
     CDS             6806..7960
                     /gene="tolA"
                     /locus_tag="OO161_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5463956.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAADK
                     KAAADKKAAEKAAAEKAAADKKAAAEKAAAAAEKAAAEADDIFGELSSGKNAPKTGGG
                     AKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAP
                     DGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            8093..9385
                     /gene="tolB"
                     /locus_tag="OO161_002968"
     CDS             8093..9385
                     /gene="tolB"
                     /locus_tag="OO161_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5463957.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQVRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            9420..9941
                     /gene="pal"
                     /locus_tag="OO161_002969"
     CDS             9420..9941
                     /gene="pal"
                     /locus_tag="OO161_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5463958.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            9951..10742
                     /gene="cpoB"
                     /locus_tag="OO161_002970"
     CDS             9951..10742
                     /gene="cpoB"
                     /locus_tag="OO161_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5463959.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
BASE COUNT         2703 a         2554 c         2910 g         2738 t
ORIGIN      
        1 caaattgaag aaagctctga tgatggtgat gaatatgact attcacccgc aaaagaagag
       61 tgggtaatta ccgcagcgaa cgcgaaaccg caatgcgata ttattcatga agcctggcag
      121 agcagggctg ttatccgcta tgacatggca gtgccgctca atttgtcaga acgcagcgcc
      181 cggcaatcca ctggcagagt aggggtggtg ttggttgtca ctcttagtca taacagcagg
      241 cgtattgatg tggatatcaa ccttgataac caggctgacg atcatcgcct tcgtgtcctg
      301 gtccctacac cttttaacac cgacagtgtt ctggcagata cgcagtttgg ttcgctaacg
      361 cgccccgtga acgacagtgc aatgaacaac tggcagcaag aaggctggaa agaagcgccg
      421 gttccggtat ggaatatgct caactatgtt gccttacagg aagggcgtaa cggcatggct
      481 gtctttagcg aagggttacg tgaatttgaa gtcatcggtg aagagaagaa aacctttgcc
      541 attacgttgc tgcgtggcgt gggcttactg ggcaaagaag atctgctttt aaggcctggg
      601 cggccttcgg gaattaaaat gccagtcccg gactcacaac tacgtggtct gctttcttgt
      661 cgcctaagtt tattgagtta taccggtacg ccaaccgccg ctggtgtagc tcagcaggcg
      721 cgagcatggc tgactccagt acagtgttac aacaaaatcc catgggatgc gatgaagctc
      781 aacaaagccg gattcaacgt gccggaaagt tatagtttgt tgaaaatgcc cccagtggga
      841 tgcctgataa gcgcacttaa gaaagctgaa gaccgacaag aagtgatttt acggctgttt
      901 aatccggctg aatcagcaac ctgtgatgcg actgttgctt tcagtcgcga ggtgatttct
      961 tgctcagaaa cgatgatgga tgaacacatt accaccgagg aaaatcaagg ttcaaatcta
     1021 tcggggcctt ttttacccgg ccagtcacgg acgttcagtt accggcttgc ctgaatagca
     1081 atcaaaccga agccacatat gcgcggccag attgttgaca aagggcgctt tgttcatgcc
     1141 ggatacggca tgaatgcttt attcggtcta caaaagcagg caaattcaat atattgcaga
     1201 gatgatgtag acactggcaa gcgtagcgca tcaggtaatt ttgcgtttat cttcactctc
     1261 aagccacgta tatgtggctt tatttttaac aaaataataa ccctgggtga gttaattata
     1321 atataattat aagttaatta aatgttaata tcggcggggg tggatttatg cctttattag
     1381 taatcctgaa actctgcgtc gtattagcca gtgaccaaaa aaagaattaa ggtcaaccgt
     1441 gctgtttttg cttcgtctct ttttatcttt aattgccaac cgaaactaat ttcagcctta
     1501 taactcacac attttaaaca taaatgtcac taaagttacc ttattgaaac atgattaaca
     1561 taatttgtag gaattgatat ttatcaatgt ataagtcttg gaaatgggca tcaaaaagag
     1621 ataaattgtt ctcgatcaaa ttggctgaaa ggcggtaatt tagctataaa ttgatcaccg
     1681 tcgaaaaatg caaatttgct tcaacaaaaa cctgtttatt gtaaggattt tgcggcgtaa
     1741 tatatacgtg ggatcaattt gagtttttat taacatgttt gcaacctttc tttacgccgt
     1801 ttttgtgtgc attcacatgg tatgatgaaa gtgttcaaac aaatttctat tggggcatgc
     1861 gtgtgaccct ttctaacggg gttcactctc ggagtcttca tgcgatgagc aaggagtcat
     1921 gatgttagat atagtcgaac tgtcgcgctt acagtttgcc ttgaccgcga tgtaccactt
     1981 cctttttgtg ccactgacgc tcggtatggc gttcctgctg gccattatgg aaacggtcta
     2041 cgtcctctcc ggcaaacaga tttataaaga tatgaccaag ttctggggca agttgtttgg
     2101 tatcaacttc gctctgggtg tggctaccgg tctgaccatg gagttccagt tcgggactaa
     2161 ctggtcttac tattcccact atgtagggga tatcttcggt gcgccgctgg caatcgaagg
     2221 tctgatggcc ttcttcctcg aatccacctt tgtaggtctg ttcttcttcg gttgggatcg
     2281 tctgggtaaa gttcagcata tgtgtgtcac ctggctggtg gcgctcggtt caaacctgtc
     2341 cgcactgtgg attctggttg cgaacggctg gatgcaaaac ccaatcgcgt ccgatttcaa
     2401 ctttgaaact atgcgtatgg agatggtgag cttctccgag ctggtgctta acccggttgc
     2461 tcaggtgaaa ttcgttcaca ctgtagcgtc tggttatgtg actggcgcga tgttcatcct
     2521 cggtatcagc gcatggtata tgctgaaagg tcgtgacttc gccttcgcta aacgctcctt
     2581 tgctatcgct gccagcttcg gtatggctgc tgttctgtct gttattgttc tgggtgatga
     2641 atccggctac gaaatgggcg acgtgcagaa aaccaaactg gctgctattg aagccgagtg
     2701 ggaaacgcaa cctgcgcctg ctgcctttac tctgttcggc attcctgatc aggaagagga
     2761 gacgaacaaa tttgcgattc agatccctta cgcactgggc atcattgcaa cgcgttccgt
     2821 ggataccccg gttatcggcc tgaaagagct aatggtgcag catgaagaac gcattcgtaa
     2881 cgggatgaag gcgtactctc tgctcgaaca actgcgttct ggttctaccg accaggcggt
     2941 tcgtgaccag ttcaatagca tgaagaaaga cctcggttac ggtctgctgc tgaaacgcta
     3001 tacgccaaac gtggctgatg cgactgaagc gcagattcaa caggcaacca aagactccat
     3061 tccgcgtgta gcgccgctgt acttcgcgtt ccgtattatg gtggcgtgtg gcttcctgct
     3121 gctggcaatc atcgcgctct ctttctggag tgtcatccgc aaccgcattg gcgagaaaaa
     3181 atggctgctg cgcgccgcgc tgtacggtat tccgctgccg tggattgctg tagaagcggg
     3241 ctggttcgtg gctgaatatg gccgccaacc gtgggctatc ggtgaagtgc tgccgacagc
     3301 tgtggcgaat tcgtcactga ccgcaggcga tctcatcttc tcaatggtgc tgatttgcgg
     3361 cctgtatacc ctgttcctgg tggcagaatt gttcttaatg ttcaaatttg cacgcctcgg
     3421 cccaagcagc ctgaaaaccg gtcgctatca ctttgagcag tcttccacga ctactcagcc
     3481 ggcacgctaa gacaggagtc gtcaaatgat cgattatgaa gtattgcgtt ttatctggtg
     3541 gctgctggtt ggcgttctgc tgattggttt tgcagtcact gacggtttcg acatgggggt
     3601 gggcatgctc acccgtttcc tcggtcgtaa cgacaccgag cgtcgaatta tgattaactc
     3661 cattgcacca cactgggacg gtaaccaggt ttggctgatc accgcgggcg gcgcactctt
     3721 tgctgcctgg ccgatggtct atgccgctgc gttctccggc ttctatgtgg cgatgatcct
     3781 cgtgctggcg tctttgttct tccgtccggt cggttttgac taccgctcca agattgaaga
     3841 aacccgctgg cgtaacatgt gggactgggg catcttcatt ggtagcttcg ttccgccgct
     3901 ggtaattggt gttgcgttcg gtaacctgtt gcagggcgta ccgttcaacg ttgatgaata
     3961 tctgcgtctg tactacaccg gtaacttctt ccagttgctt aacccgttcg gcctgctggc
     4021 aggcgtggtg agcgtaggga tgatcattac tcagggcgca acctatctgc aaatgcgtac
     4081 cgtgggcgaa ctgcacctgc gtacccgtgc aacggctcag gtggctgcgc tggtgacact
     4141 ggtctgtttc gcactggctg gcgtatgggt gatgtacggt atcgatggtt atgtcgtgaa
     4201 atcgacaatg gaccattacg cagcctctaa cccactgaat aaagaagtgg ttcgtgaagc
     4261 tggcgcatgg ctggttaact tcaacaacac gccaattctg tgggctattc cggcactggg
     4321 tgtggttctg ccgctgctga ccatcctgac tgcacgtatg gataaagccg cgtgggcgtt
     4381 tgtgttctcc tccctgacgc tggcctgcat catcctgaca gccggtatcg caatgttccc
     4441 gtttgtgatg ccgtccagca ccatgatgaa cgcaagtctg acaatgtggg atgcaacttc
     4501 cagccagttg acgcttaacg tcatgacctg ggttgcggtg gttctggtac cgatcattct
     4561 gctctacacc gcctggtgtt actggaaaat gttcggtcgt atcaccaaag aagatattga
     4621 acgtaacacc cactctctgt actaagtaag gagctaaaaa tgtggtattt cgcatggatt
     4681 ctgggaacgc ttcttgcctg ttcgtttggg gtaatcaccg cgctggcgct tgaacacgtc
     4741 gaatcaggca aagccggtca agaagacatc tgatgagtaa gattatcgcg accttgtatg
     4801 cggtaatgga caagcgcccc ctgcgggcgc tttccttcgt gatggcgctt ctgttagcag
     4861 gatgtatgtt ttgggaccca tctcgttttg ccgcgaagac cagtgaactg gaaatctggc
     4921 atggtttatt gctgatgtgg gccgtctgtg ctggtgtgat tcacggcgtg ggctttcgtc
     4981 cgcagaaggt tctttggcaa gggatttttt gcccattgct tgccgatatt gttctcatta
     5041 tcgggctgat tttcttcttc ttttaaatca gaattctctt taaaaaatta tgggccgctc
     5101 caggcccata aatttttacg ctcccttaac ttgccctcat tcccaaacct caatcgcgcg
     5161 cgtatagtag cagcgtttaa aagctctaac ttttgttgca ttaccgggat gtaaagtgaa
     5221 tacaacgctg tttcgatggc cggttcgcgt ctactatgaa gataccgatg ccggtggtgt
     5281 ggtgtaccac gccagttacg tcgcttttta tgaaagagca cgcacagaga tgctgcgtca
     5341 tcatcacttc agtcagcagg cgctgatggc tgaacgcatt gcctttgtgg tacgtaaaat
     5401 gacggtggaa tattacgcac ctgcgcggct cgacgatatg ctcgaaatac agactgaaat
     5461 aacatcaatg cgtggcacct ctttggtttt cacgcaacgt attgtcaacg ccgagaatac
     5521 tttgctgaat gaagcagagg ttctggttgt ttgcgttgac ccactcaaaa tgaagcctcg
     5581 tgcgcttccc aagtctattg tcgcggagtt taagcagtga ctgacatgaa tatccttgat
     5641 ttgttcctga aggctagcct tctggttaaa cttatcatgt tgattttgat tggtttttca
     5701 atcgcatcct gggccattat tatccagcgg acccgtattc ttaacgcagc ggcgcgcgaa
     5761 gccgaagcgt ttgaagataa attctggtct ggaatcgaac tctctcgcct ctatcaagag
     5821 agtcagggga aacgggataa tctgactggt tcggaacaaa tattttacag cgggttcaaa
     5881 gagtttgtgc gcctgcatcg tgccaatagc catgcgccgg aagccgtagt ggaaggggcg
     5941 tcgcgtgcta tgcgtatctc catgaaccgt gaacttgaaa atctggaaac gcacattccg
     6001 ttcctcggta cggttggctc catcagcccg tatattggtc tgtttggtac ggtctggggg
     6061 atcatgcacg cctttatcgc cctcggggcg gtaaaacaag caacactgca aatggttgcg
     6121 cccggtatcg cagaagcgtt gattgcgact gcaattggtc tgtttgccgc tatcccggca
     6181 gttatggcct acaaccgcct caaccagcgc gtaaacaaac tggaactgaa ttacgacaac
     6241 tttatggaag agtttaccgc gattctgcac cgccaggcgt ttaccgttag cgagagcaat
     6301 aaggggtaag tcatggccag agcgcgtgga cgaggtcgtc gcgaacttaa gtccgaaatc
     6361 aacattgtac cgttgctgga cgtactgctg gtgctgttgc tgatcttcat ggcgacagcg
     6421 cccatcatca cccagagcgt ggaggtcgat ctgccagacg ctactgaatc acaggcggtg
     6481 agcagtaacg ataatccgcc agtgattgtt gaagtgtctg gtattggtca gtacaccgtg
     6541 gtggttgaga aagatcgcct ggagcgttta ccaccagagc aggtggtggc ggaagtgtcc
     6601 agccgtttca aggccaaccc gaaaacggtc tttctgatcg gtggcgcaaa agatgtgcct
     6661 tacgatgaaa taattaaagc actgaacttg ttacatagtg cgggtgtgaa atcggttggt
     6721 ttaatgacgc agcctatcta aacatctgcg tttcccttgc ttgaaagaga gcgggtaaca
     6781 ggcgaacagt ttttggaaac cgagagtgtc aaaggcaacc gaacaaaacg acaagctcaa
     6841 gcgggcgata attatttcag cagtgctgca tgtcatctta tttgcggcgc tgatctggag
     6901 ttcgttcgat gagaatatag aagcttcagc cggaggcggc ggtggttcgt ccatcgacgc
     6961 tgtcatggtt gattcaggtg cggtagttga gcagtacaaa cgcatgcaaa gccaggaatc
     7021 aagcgcgaag cgttctgatg aacagcgcaa gatgaaggaa cagcaggctg ctgaagaact
     7081 gcgtgagaaa caagcggctg aacaggaacg cctgaagcaa cttgagaaag agcggttagc
     7141 ggctcaggag cagaaaaagc aggctgaaga agccgcaaaa caggccgagt taaagcagaa
     7201 gcaagctgaa gaggcggcag cgaaagcggc ggcagatgct aaagcgaagg ccgaagcaga
     7261 tgctaaagct gcggaagaag cagcgaagaa agcggctgca gacgcaaaga aaaaagcaga
     7321 agcagaagcc gccaaagccg cagcgcagaa aaaagccgag gcagccgctg cggcactgaa
     7381 gaagaaagcg gaagcggcag aagcagctgc agctgaagca agaaagaaag cggctgctga
     7441 aaaggctgca gctgataaga aagcggcagc cgacaaaaaa gcagcagaaa aagcggctgc
     7501 tgaaaaggca gcagctgata agaaagcagc ggcagaaaaa gccgccgcag cggcagaaaa
     7561 agccgccgca gaggcagatg atattttcgg tgagctaagc tctggtaaga atgcaccgaa
     7621 aacgggggga ggggcgaaag ggaacaatgc ttcgcctgcc gggagtggta atactaaaaa
     7681 caatggcgca tcaggggccg atatcaataa ctatgccggg cagattaaat ctgctatcga
     7741 aagtaagttc tatgacgcat cgtcctatgc aggcaaaacc tgtacgctgc gcataaaact
     7801 ggcgcccgat ggtatgttac tggatatcaa acctgaaggt ggagatcccg cactttgtca
     7861 ggctgcgttg gcagcagcta aacttgcgaa gatcccgaaa ccaccaagcc aggcagtata
     7921 tgaagtgttc aaaaacgcgc cattggactt caaaccgtaa tcgcgatgtt gactgttcgg
     7981 acggtcaaca tcaggcaccg gttgccacgg ggttctggta gttttgtgta ttttagtttg
     8041 ttaacattct gctaaattat cgtgggccat cggtccagat aagggagata tgatgaagca
     8101 ggcattacga gtagcatttg gttttctcat actgtgggca tcagttctgc atgctgaagt
     8161 ccgcattgtg atcgacagcg gtgtagattc cggtcgtcct attggtgttg ttcctttcca
     8221 gtgggcgggg cctggtgcgg cacctgaaga tattggcggc atcgttgctg ctgacttgcg
     8281 taacagcggt aaatttaatc cgttagatcg cgctcgtctg ccacagcagc cgggtagtgc
     8341 gcaggaagta caaccatctg catggtccgc actgggcatt gacgctgtag ttgtcggtca
     8401 ggtcactccg aatccggatg gttcttacaa tgttgcttat caacttgttg acactggcgg
     8461 cgcaccgggt actgtacttg ctcagaactc gtacaaagtg aacaagcagt ggctgcgtta
     8521 tgctggtcat accgccagtg atgaagtgtt tgaaaaactg accggcatta aaggtgcgtt
     8581 ccgtacccgt attgcctacg ttgttcagac caacggcggt cagttcccgt atgaactgcg
     8641 cgtatctgac tatgacggtt acaaccagtt tgtcgttcac cgttcaccgc agccgctgat
     8701 gtctccggcg tggtcaccag acggttctaa actggcttat gtgaccttcg aaagcggtcg
     8761 ttccgcgctg gttattcaga cgctggcaaa tggcgctgta cgtcaggtgg cttcattccc
     8821 gcgtcacaac ggtgcacctg cattctcgcc agacggcagc aaactggcat tcgccttgtc
     8881 gaaaaccggt agtctgaacc tgtacgtaat ggatttggct tctggtcagg tccgccaggt
     8941 gactgatggt cgcagtaaca ataccgaacc gacctggttc ccggatagcc agaacctggc
     9001 atttacttct gaccaggccg gtcgtccgca ggtttataaa gtgaatatca acggcggtgc
     9061 gccacaacgt attacctggg aaggttcgca gaaccaggat gcggatgtca gcagcgacgg
     9121 taaatttatg gtaatggtca gctccaatgg tgggcagcag cacattgcca aacaagatct
     9181 ggcaacggga ggcgtacaag ttctgtcgtc cacgttcctg gatgaaacgc caagtctggc
     9241 acctaatggc actatggtaa tctacagctc ttctcagggg atgggatccg tgctgaattt
     9301 ggtttctaca gatgggcgtt tcaaagcgcg tcttccggca actgatggac aggtcaaatt
     9361 ccctgcctgg tcgccgtatc tgtgataata attaattgaa tagtaaagga atcattgaaa
     9421 tgcaactgaa caaagtgctg aaagggctga tgattgctct gcctgttatg gcaattgcgg
     9481 catgttcttc caacaagaac gccagcaatg acggcagcga aggcatgctg ggtgccggca
     9541 ctggtatgga tgcgaacggc ggcaacggca acatgtcttc cgaagagcag gctcgtctgc
     9601 aaatgcaaca gctgcagcag aacaacatcg tttacttcga tctggacaag tacgatatcc
     9661 gttctgactt cgctcaaatg ctggatgcac atgcaaactt cctgcgtagc aacccgtctt
     9721 acaaagtcac cgtagaaggt cacgcggacg aacgtggtac tccggaatac aacatctccc
     9781 tgggtgaacg tcgtgcgaac gccgttaaga tgtacctgca gggtaaaggc gtttctgcag
     9841 accagatctc catcgtttct tacggtaaag aaaaacctgc agtactgggt catgacgaag
     9901 cggcatactc caaaaaccgt cgtgcggtac tggtttacta agagaattgc atgagcagta
     9961 acttcagaca tcaactattg agtctgtcgt tactggttgg tatagcggcc ccctgggccg
    10021 cttttgctca ggcaccaatc agtagtgtcg gctcaggctc ggtcgaagac cgcgtcactc
    10081 aacttgagcg tatttctaat gctcacagcc agcttttaac ccaactccag caacaacttt
    10141 ctgataatca atccgatatt gattccctgc gtggtcagat tcaggaaaat cagtatcaac
    10201 tgaatcaggt cgtggagcgg caaaagcaga tcctgttgca gatcgacagc ctcagcagcg
    10261 gtggtgcagc ggcgcaatca accagcagcg atcaaagcgg tgcggcggca tcaacgacgc
    10321 cgacagctga tgctggtact gcgaatgctg gcgcgccggt gaaaagcggc gatgcaaaca
    10381 cggattacaa tgcagctatt gcgctggtgc aggataaatc ccgccaggat gacgcaatgg
    10441 tggcatttca gaatttcatc aaaaattacc ctgattcaac ttacctgcca aacgccaatt
    10501 attggctggg tcagttaaac tacaacaagg gtaaaaaaga tgatgcggcg tactattttg
    10561 cttcggtagt gaaaaactat ccgaagtcac caaaggctgc tgatgcgatg tttaaagtcg
    10621 gcgtcatcat gcaggacaaa ggtgacaccg caaaagcgaa agccgtatac cagcaggtta
    10681 tcagtaaata ccctggtact gatggcgcta aacaggcaca aaaacgtctg aacgcgatgt
    10741 aatgcataac acacgaccag aagttgcatt atttctggtc gtgtcgtgcg aatcataagc
    10801 agttgagtga tctgcatcga aatttttgtt gcgctcaagt ctgaaatcag taatatatgc
    10861 cgccgttgcc acgggatatc aaacaaaccg aaagcaacga aaaag
//
LOCUS       DAJTNA010000133        10899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTNA010000133 DAJTNA010000000
VERSION     DAJTNA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.134"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(228..2087)
                     /gene="gss"
                     /locus_tag="OO161_002971"
     CDS             complement(228..2087)
                     /gene="gss"
                     /locus_tag="OO161_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5463960.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLN
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSCARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2292..3158
                     /gene="yghU"
                     /locus_tag="OO161_002972"
     CDS             2292..3158
                     /gene="yghU"
                     /locus_tag="OO161_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5463961.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFFHYAPVKTEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="OO161_002973"
     CDS             complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="OO161_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5463962.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="OO161_002974"
     CDS             complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="OO161_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5463963.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="OO161_002975"
     CDS             complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="OO161_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5463964.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4357..4851)
                     /locus_tag="OO161_002976"
     CDS             complement(4357..4851)
                     /locus_tag="OO161_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5463965.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="OO161_002977"
     CDS             complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="OO161_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5463966.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="OO161_002978"
     CDS             complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="OO161_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5463967.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGDLIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLCVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLITIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="OO161_002979"
     CDS             complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="OO161_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5463968.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVYYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="OO161_002980"
     CDS             complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="OO161_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5463969.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10015..10302)
                     /locus_tag="OO161_002981"
     CDS             complement(10015..10302)
                     /locus_tag="OO161_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5463970.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10421..>10899)
                     /locus_tag="OO161_002982"
     CDS             complement(10421..>10899)
                     /locus_tag="OO161_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5463971.1"
                     /translation="GGYPGNDDKGRELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVG
                     ITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADVAKIEAPLLLHFAELDTRINEG
                     WPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
BASE COUNT         2660 a         2992 c         2689 g         2558 t
ORIGIN      
        1 caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt atcagacaat
       61 aagcattcat tcaaaacctt tagaaataat caaaacacag gtattccact ggttaatata
      121 ttcacatatg aaatgaataa atattagaac atataaatat tgaatatttt gattaatccc
      181 cgtactgatt attcttcata atcagtacgg ggttgctaca acatgtatta ctttttcacc
      241 acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc accgcgcaaa
      301 cacgtcccac cgtagttgcc gcccacggtg aaggtacata cctgaatgta tttaccgtcc
      361 actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa tttaccgctg
      421 gttttgtcca gcacctcttc atgatggctg accaggtcga tattgctacc acagcgaccg
      481 gcaatcggtt tcactgcgta acctgttttc accaattcat cattaacagt gaaatcggta
      541 tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat cgctttgttg
      601 ccggggatca ccgtccacag cggctcaaag actagcactt ccgggcgcag caatacgtcg
      661 ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc aaactcacgg
      721 tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt ccacacgcag
      781 ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc
      841 aatccacgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc
      901 gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacaa
      961 ctgtgtttcc aggcaccagc caattcgtta atcagccctt ccgccggatt gaagccgttg
     1021 cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag
     1081 gcggagtcgg cgttgtactc gtaaaccttc aggccacgct catccatgca gaaatccata
     1141 cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg tggccagagg
     1201 attttcggga tgtcgaacag cgccagcagg ttgtcatctt tcagtacctt gtcggttgcg
     1261 ttaagataca tcaggtgcag ctcgttggtg gctttaatta gctcctgctc ggcactctcg
     1321 gtaatggtgt agtaatgata aggatcctga ttgatcacct gaccgttggc ctgcacatag
     1381 gcgttttgca gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc
     1441 aggcgcgctc cgctgatttt cagcagctcg cctgcaattt ccggctgcgg taagctgtat
     1501 tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa agtgtctttc
     1561 agggtatagc agccgttttc gaccaccatc tccagctcac gcgtccactg ttgcccttgc
     1621 ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg
     1681 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
     1741 gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg
     1801 tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca
     1861 gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac gcattgccac
     1921 ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
     1981 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
     2041 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
     2101 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
     2161 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
     2221 aaaattcaca ttctgtacag caagtgacct gccacactgc ttcaacacta ccactcagaa
     2281 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
     2341 ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc tggtccgacg catgaaaaaa
     2401 cgctgcccgt tggcaaacac ccattgcaac tttattcgct gggaacgccg aacggtcaga
     2461 aagtaacgat tatgcttgag gagctgctgg cgctgggcgt tactggtgca gagtacgacg
     2521 cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc
     2581 caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc cgtgtgtttg
     2641 aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatt
     2701 tggcaaagcg tactgaaacg atgaactggc tgttctggtt acagggcgcg gcaccgttcc
     2761 tcggcggtgg ttttggtcac tttttccatt acgcaccggt aaagactgag tacgccatca
     2821 accgctttac catggaagcc aaacgtctgc tcgacgtgct ggataagcaa ctggcgcagc
     2881 ataagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg
     2941 gcaacgtggt gttaggtggt gtgtatgatg ccgctgagtt tcttgatgcg ggcagttata
     3001 agcatgtaca acgctgggcg aaagaagtag gcgaacgtcc ggcggtgaaa cgtgggcgta
     3061 ttgttaaccg caccaacgga ccgctgaatg agcagttgca tgagcgccat gacgccagtg
     3121 atttcgagac gaatacggaa gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa
     3181 gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca atgcctgatg
     3241 cgacgctgcc gcgtcttatc aggcctacga aagctaatca tcacgcgctg gtaatgtcgt
     3301 aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca
     3361 tggcaaatcc gacgtgtacc agcacccact ggcccagtag atcggcaggg ttaccttcac
     3421 aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagctggt
     3481 gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt attaactccg
     3541 gttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct
     3601 cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat
     3661 cccagcacca ggcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg
     3721 ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg
     3781 cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt
     3841 ggataatttc aacggcgctc tggcaaagcg acaactcatg cattttcgcc actcctgcga
     3901 ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa
     3961 aggatcattc gtgcgcagtc atccgtcagg cgttggccct cttcaatcga catactgtgc
     4021 gaaagcggtg acatcagcga acaggagagg tattgcgaaa caccgtccag ttcaccaacg
     4081 gtaaaggtca tggtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc
     4141 ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca
     4201 caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga
     4261 tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact
     4321 tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg
     4381 gatggcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg caggttcaat
     4441 cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg ggatcacgcc
     4501 gaccagggtc agctttttcg gaaactcacc ggtgaagcgc agggccgaca atacgtcggc
     4561 caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg
     4621 caggatcatc atcgttcccg gcgtgttctt tttcgacaca atggcatccg caataatcag
     4681 atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat
     4741 ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat
     4801 ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc
     4861 ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac
     4921 gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc agcaaccggt
     4981 gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cggaccagag
     5041 ttccaggttg atggaacaac tgcctggtag ttactgataa taccgtcttt aataaccatc
     5101 cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt
     5161 gccggaatgt tcggtttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt
     5221 gcactgtatt gattttgcag gatatcctgc aattcgcagc agtgaacggt acgaccaata
     5281 atacggccca gcgtggagtg cagctgcgcc acttccagcg tgttgccagt cagtttctga
     5341 taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc cgccagtttc
     5401 accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc
     5461 acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc
     5521 cacggtgcct gcggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct
     5581 ttgatcaggt attcatctga atgagaagtg atcggacgat acgaggacag atccgcattc
     5641 tcaatgtagc cgcccgggaa caggaagctg ccgtttttac tgtcggtcgg gaattccggc
     5701 acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcggca
     5761 ataactgcgg tatcaacctt ataaacctgt tcaacaaagt cgctcagctt gtcgatgaaa
     5821 gacttgatgt acatcaggcg ctcaaggttc agcacgccca aaccgtcgag gttgatcggg
     5881 ttcgcgacac cacctaccgc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc
     5941 acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc
     6001 aggttcactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt ggcgaaaata
     6061 cccaactgac cgctggcaac cagatctttg atcttgttct gaactttagt gaactcttcc
     6121 ggactgttca gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggttgggtca
     6181 gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg
     6241 atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc
     6301 ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta
     6361 gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc
     6421 acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca
     6481 ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta
     6541 attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag
     6601 gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac
     6661 caatagtaat caacagttct tcccaggtcg ggaagtaggc ataaccgccg cccgggttga
     6721 atgccaccag cgaataggtc agacgccagg ttgcacaacc taacagtgcg ctcagtgctg
     6781 acaggaacag catgcgggaa tcattacgca gcttcgccac acacagaacg accagcggga
     6841 agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta
     6901 acgacagctt gtcgcgatag ataagatcgc caaagcgcag cacgatgaaa atcgccagca
     6961 acacactgat ggtgttggtc agcttgacga acagactctt ttcatccgga ccgttgccac
     7021 gcagacccgc ctgcaccagc gaaccttcaa agatgacaat cgagaagccc atgatgaacg
     7081 ccgtcagcag cgagaacagc ggcaacattt catagctctg ccacaacgga tgcaccttgt
     7141 agcccgccga gatcatcagt gaccccattg aagactggtg catggtcggc aacagcgcgc
     7201 caagtgcaat gatgaagaac atcaccttgt tcagacgctt gagagaaacc ttccagccca
     7261 gacgttcaaa cagtgccgga gcaaactcca gtgccatcac gccgatatag atggtcatac
     7321 agaccgccgt ctcgaacagt accgagttca cgttgaagtg ccccggaatg tagaagtacg
     7381 gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gagtaaccaa
     7441 acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt
     7501 ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtaccaatc agcaggtcaa
     7561 acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga
     7621 acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga
     7681 ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct
     7741 ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc cgccagcaca
     7801 gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg
     7861 gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc
     7921 agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc
     7981 gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca
     8041 ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accgaaaatc
     8101 accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc
     8161 acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg
     8221 tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt gcacacgtct
     8281 ttgtcgtaat agacaatgcc ggttttcgga tcttttttca gtgcagagac cgggcacaca
     8341 gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc
     8401 tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta
     8461 tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg
     8521 aagttgatat cctgacactt ggtgacgcaa gcttggcagc ctacgcacaa ggtcgagtcg
     8581 tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca
     8641 gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg
     8701 ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac
     8761 gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag
     8821 cgcctgcaga gacgttgccg ccctctttag cgttaacatc cggtttctgt gaacgcggag
     8881 tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata ccttcttcgt
     8941 tacagccata gcatgggtga ccaatcgcta ccggccacac gccgccaaca tcgcagaatt
     9001 gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc
     9061 agccttcacg gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg
     9121 ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cgatttttgt
     9181 catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt
     9241 gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca
     9301 ggctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac gcagagcagg
     9361 aaccgatagc gataatcgct gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg
     9421 gctcaccagc aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacaa
     9481 ccaacacata ctgccctttg tacttctcaa gtgcgttatg tttgttctct tcgacctgat
     9541 gaccgaaggc ggcggaaagc acttcgtgat actccagaga gatagtctcc agcacgaggt
     9601 tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg
     9661 cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag
     9721 cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc ataaaatcac
     9781 gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg
     9841 ttgcgaagac ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc
     9901 catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac
     9961 gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt
    10021 ttcgaggcga taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa
    10081 gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca
    10141 ggcgctaagc tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa
    10201 accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc
    10261 ttttaatccc gccattaagc ctttgccaaa atggttattc atcgttcacc tcattcgcta
    10321 tatattatca tatatagcga tattttaggc aaagaacgac ttttgcgcac actatttttt
    10381 catgcttgtt taagacgtaa tcccgtaaga gataaaccca tcaggagaga tatttatcga
    10441 accatttcag cgtcctttgc caggcaagat cggcggcaga tttgtcataa cgaggcgtgg
    10501 aatcattatg gaatccgtga ttaacccccg gatagatata cgcctcatag accttattat
    10561 tggctttcaa cgccgcctca taagcgggcc agccctcgtt gattcgggtg tccagttccg
    10621 cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac
    10681 cataaaacgg caccgcgcag gccagttccg gatacgcgac agccgccgcg ttcgatacgc
    10741 caccgccata gcaaaatccg gtaataccca ctttgcctgt cgcttgcgga tagcgttgca
    10801 taaactcaat tgcggcaaag aaatcattca tcagtttggt tggatcaacc tgctgttgca
    10861 gctcacgacc tttatcatca tttcccggat aacctccaa
//
LOCUS       DAJTNA010000134        10655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTNA010000134 DAJTNA010000000
VERSION     DAJTNA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.135"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..576)
                     /locus_tag="OO161_002983"
     CDS             complement(<1..576)
                     /locus_tag="OO161_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5463972.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRGVTKQNGDGYA"
     gene            1143..1508
                     /locus_tag="OO161_002984"
     CDS             1143..1508
                     /locus_tag="OO161_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5463973.1"
                     /translation="MKKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1548..2243
                     /locus_tag="OO161_002985"
     CDS             1548..2243
                     /locus_tag="OO161_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR5463974.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQRNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            2310..3728
                     /gene="dcm"
                     /locus_tag="OO161_002986"
     CDS             2310..3728
                     /gene="dcm"
                     /locus_tag="OO161_002986"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5463975.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQIVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3709..4179
                     /gene="vsr"
                     /locus_tag="OO161_002987"
     CDS             3709..4179
                     /gene="vsr"
                     /locus_tag="OO161_002987"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5463976.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4168..5088)
                     /gene="yedA"
                     /locus_tag="OO161_002988"
     CDS             complement(4168..5088)
                     /gene="yedA"
                     /locus_tag="OO161_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5463977.1"
                     /translation="MRFRQLLPLFGTLFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFSIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            5261..6178
                     /locus_tag="OO161_002989"
     CDS             5261..6178
                     /locus_tag="OO161_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5463978.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            6257..6439
                     /locus_tag="OO161_002990"
     CDS             6257..6439
                     /locus_tag="OO161_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5463979.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6610..8304
                     /gene="yedQ"
                     /locus_tag="OO161_002991"
     CDS             6610..8304
                     /gene="yedQ"
                     /locus_tag="OO161_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR5463980.1"
                     /translation="MQHETKMENQSWLKKIARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQCNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFSHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8301..9116)
                     /locus_tag="OO161_002992"
     CDS             complement(8301..9116)
                     /locus_tag="OO161_002992"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCR5463981.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGVPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9414..9641)
                     /gene="yodD"
                     /locus_tag="OO161_002993"
     CDS             complement(9414..9641)
                     /gene="yodD"
                     /locus_tag="OO161_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5463982.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9804..9992
                     /gene="dsrB"
                     /locus_tag="OO161_002994"
     CDS             9804..9992
                     /gene="dsrB"
                     /locus_tag="OO161_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5463983.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10036..>10655)
                     /gene="rcsA"
                     /locus_tag="OO161_002995"
     CDS             complement(10036..>10655)
                     /gene="rcsA"
                     /locus_tag="OO161_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5463984.1"
                     /translation="TIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACDSQ
                     RPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKILLIS
                     SKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISDQM
                     NIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
BASE COUNT         2724 a         2514 c         2749 g         2668 t
ORIGIN      
        1 cgcatagcca tcaccgtttt gtttagtaac gccacgattg ctgttcttac cttgatattg
       61 cacagcaaaa ttcagaccat caaccagacc aaagaaatca gagttacggt aggttgcaac
      121 accgttggta cgaccgttca tgaagttgtc agcggaagcc caggaatcac caccccattc
      181 aactaacata tcggtataag ctgcggcatc ataagcaaca ccgtagttac gaccatagtc
      241 gaaagatcct aactcgttat atttcaaacc tgcgaaggtt aaacgggttt tttcctgatt
      301 agagccttcg ggcttgctgg cgtcgaggtt gtattcaaac tgaccaaaac cagtcagttc
      361 gctggtgatt tgggtttcac ctttaacacc gaaacgagca taggaggtat cttcgttttc
      421 gctgttatta tcatcagtat ttgaccagat gcgttcgcca accatcttgc cgtagaaatc
      481 aactttattg ccatctttat tgtaaatttc agccgcattt gctgcgccag caactaataa
      541 cgccgggacc agcattgcca gaacttttct tttcattatg tattcccttg tgattataat
      601 cttcatgaat atatcaataa gtgccgttat ccaaaaaaaa gcacatttgg atactattct
      661 atgaagttca ttttatttta agcattacat gcaacaaatg tatttaaaag atttcaaaat
      721 gtttctaatc tatttaatat aattcagaat tatgaattct aaatataaat attcatgaaa
      781 attataaatg tatatcaata tgttatgagt tgagcggaaa catagggggt atcaatgatt
      841 gttgcgccaa acggctggtg tgatcaggcg cacattaatg aaggcataaa aaaaagccgg
      901 catcatgccg gcccaaaaaa tataaacaag acgataaaag gctaataacg gaagcatcat
      961 gacacagaat aataaaaagg tataattgta atcagtgcta atatattgat taaatgaatt
     1021 aataaagccg atatcaaaga taaataaaat cgctcaaaac gatttttcct gtattcactg
     1081 ccgttgcgca aaatttatct atttgttcaa aaaatgattg agtcttgact ggctcatcca
     1141 atgtgaaaaa atgtgacttt tatcacataa tagtactaag tctgaatttt ccgggttatc
     1201 tcaaaatgga atacggttcg acaaagatgg aagagagact ctctcgcagc cctggcggca
     1261 aactggcttt gtgggctttc tatacatggt gtggctattt cgtctgggca atggcgcgtt
     1321 acatctgggt gatgagccgg atccccgatg cccccgtgtc gggatttgaa agcgatttag
     1381 gctctaccgc gggaaagtgg ttaggggcac tggtcggatt tttatttatg gctctggtcg
     1441 gggcattgtt aggcagcatt gcctggtaca cccgacctcg tcccgcacgt agcagacgct
     1501 atgaataaaa actgctaagg tggcgactct ttggcaacgg agcgcgtatg gacttacaac
     1561 actggcaggc acagttcgaa aactggttga aaaaccatca tcagcaccag gacgcggccc
     1621 atgacgtgtg tcattttcgc cgcgtctggg ctacggcaca gaagctggcg gcagatgacg
     1681 atgtcgatat gctggtgatt ttaaccgcct gttattttca cgatattgtg agcctggcga
     1741 aaaatcatcc gcaaaggcag cgttcttcaa tcctggcggc agaagagacg cgtcgcctgc
     1801 tccgtgaaga gttcgagcaa tttccggcgg agaaaatcga ggccgtttgt catgtcattg
     1861 ccgctcacag tttcagcgcg caaatcgccc ccttaacaac ggaagctaaa atcgtccagg
     1921 atgcggatcg gctggaagcc ctgggggcga ttggcctggc gcgcgtgttt gccgtttcag
     1981 gggcattggg cgtggcgctg tttgatggtg aagatccgtt cgcacagcat cgcccgcttg
     2041 atgataaacg ctacgcgctg gatcatttcc agactaagtt gctgaagcta ccgcaaacca
     2101 tgcaaaccgc aagaggcaag cagctggcgc agcgcaacgc gcatttttta gtcgagttta
     2161 tggcgaagct cagtgccgaa ctggcggggg agaatgaagg tgtcgatcac aaggtgatag
     2221 atgcgttttc acccgcggga taagcgcgtg gccctaaatg gctgtaatta tgttaacctg
     2281 tcggccatct cagatggccg gttaaatcta tgcaggaaaa tatatcagta accgattcat
     2341 acagcaccgg gaatgccgca caggcaatgc tggagaaact gctgcaaatt tatgatgtta
     2401 aaacgctggt ggcgcagctt aatggtgtgg gtgagaatca ctggagcgcg gcaattttaa
     2461 aacgtgcgct ggcgaatgac tcggcatggc accgtttaag tgagaaagag ttcgcccatc
     2521 tgcaaacgtt gttacccaaa ccaccggcac atcatccgca ttatgcgttt cgctttatcg
     2581 atctatttgc cggaattggc ggcatccgtc gcggttttga atcgattggc ggacaatgcg
     2641 tgtttaccag cgaatggaac aaacatgcgg tacgcactta taaagccaac cattattgcg
     2701 atccggcgac gcatcatttt aatgaagata tccgcgatat caccctcagc cataaagaag
     2761 gcgtgagtga tgaggcggcg gcggaacata ttcgtcaaca cattcctgaa cacgatgttt
     2821 tactggccgg tttcccttgt cagccatttt cgctggctgg cgtatcgaaa aagaactcgc
     2881 tcgggcgggc gcacggtttt gcctgcgata cccagggcac gctgtttttt gatgtggtac
     2941 gcattatcga cgcgcgtcgt ccggcgatgt ttgtgctcga aaacgtcaaa aacctgaaaa
     3001 gtcacgacca gggtaaaacg ttccgcatca tcatgcagac gctggacgaa ctgggctatg
     3061 acgtggctga tgcagaagat aacgggccgg acgatccgaa aatcatcgat ggtaaacatt
     3121 ttctgccgca gcaccgtgaa cgcatcgtgc tggtgggttt tcgtcgcgat cttaatctga
     3181 aagccgattt tactctgcgt gatatcagcg aatgtttccc tgcacagcga gtgacgctgg
     3241 cgcagctgtt ggacccgatg gtcgaggcga aatatatcct gacgccggtg ctgtggaagt
     3301 acctctatcg atatgcgaaa aaacatcagg cgcgcggtaa cggcttcggt tatggaatgg
     3361 tttatccgaa caatccgcaa atcgtcacgc gtacgctgtc tgcgcgttat tacaaagatg
     3421 gcgcggaaat tttaatcgat cgcggctggg atatggccaa aggtgagaaa gactttgacg
     3481 atccgctgaa tcagcaacat cgtccacgtc ggttaacgcc tcgggaatgc gcgcgcttaa
     3541 tgggttttga agcgccggga gaagcgaaat tccgcattcc ggtttcggac actcaggcct
     3601 atcgccagtt cggtaactcg gtggtcgtgc cggtctttgc cgcggtggca aaactgcttg
     3661 agccaaaaat caaacaggcg gtggcgttgc gtcagcaaga ggcacaacat ggccgacgtt
     3721 cacgataagg ccactcgcag caaaaatatg cgcgcgattg ccacgcgtga tacggcgatt
     3781 gagaagcgcc tcgccagtct gttaaccggg cagggcctgg cgtttcgcgt tcaggacgcc
     3841 agtctgcccg gaagcccgga ttttgtcgtt gatgaatatc gctgcgtgat atttacccat
     3901 ggctgcttct ggcatcatca tcactgctat ctgtttaaag tgcctgcgac tcgaaccgag
     3961 ttctggctgg agaagatagg taaaaatgtt gaacgcgatc gccgcgatat cagtcgtttg
     4021 caggaactcg gctggcgcgt actgattgtc tgggagtgtg cattacgtgg gcgcgagaag
     4081 ctgacggatg aagcgcttac cgagcgtctg gaagagtgga tctgcggcga aggtgccagc
     4141 gcgcagatcg acacgcaggg gattcattta ctcgcttgat gcgtcctgaa taactggcgc
     4201 aactacgggt tttgccggga agagatattt tcccaacgtg accagtacca ccgcgaagac
     4261 aattacgccg agcgccagcc attcaatctt cgacagcgtc tctccgccca gtcccgtacc
     4321 cagcaagacc gcgaccaccg ggttaacgta ggcgtagctg gtggcgagag ccggactgac
     4381 attacggatt aaatacatat aagcgttgat ggcgataatc gaaccaaaca gcgccagata
     4441 gccgaccgca aggaagcctg aaagggaagg gagcgccgtc agtttttcac ctgcaatcat
     4501 cgacgcgatc attaacacca cgcctgccgc cagcatctca atcgcacccg ccatcatccc
     4561 tacaggtaag gtaatgcgcg agccataaac cgagccaaac gcccagctaa tcgagccgat
     4621 taaaatcaga atcgcgcccc acggattgcc gcttaaattt ccaccgctat tgagcatgat
     4681 gattccggca agcccaatgg cgatacccac ccattccaat ttgcgcgttt taatgctaaa
     4741 caggcggctg aagcacaggg taaagagggg cacggttgca accacaacgg cggcgatgcc
     4801 ggaaggaaca ttttgatgct cggcaaccgt caccatgcca ttaccgacag ccagcaataa
     4861 caggccaatc agcgcggcat tgagcagcgg acgtagcggg gggagcttgt gtccgcgcag
     4921 tagcaaaaat gccatcaata aaataccggc tgccaggaat cgaacgcctg ccatcattaa
     4981 cggaggccag ctttccacgc caatccgaat gacaaaatag gttgagcccc aaatgatata
     5041 caacgcaaac agcgtgccaa aaagcggtaa caactggcgg aaacgcataa tccctcacgg
     5101 tagaaataaa aaggtggttc atagtaaacg tgaaaatcat tctgctggcg agagatataa
     5161 ttgcacttga ttgttaaaaa aatgttgacc tgtgaagcaa gtatcagagc tgccgttttt
     5221 gcttcatact tacacccttc aacaataaaa atgagaggga atgcttttgg ccgggagtag
     5281 tttactgacg ttgctcgatg atatcgccac actactggac gatatctccg tgatgggcaa
     5341 actggcggcg aagaaaaccg ccggtgtatt aggggatgac ttatcgctca atgcgcaaca
     5401 agtttcaggc gtgcgggcca accgggaact tcccgtggtc tggggcgtgg cgaaaggatc
     5461 gctgattaat aaagtgattc tggtgccgct ggcgctgatc atcagtgcgt ttatcccgtg
     5521 ggcgattacg cctctgttga tgattggtgg cgcgtttctc tgctttgaag gagtagagaa
     5581 agtgctgcat atgctggagg cgcgtaaaca taaagaagat ccggcgcaga gccagcagcg
     5641 tctggagaag ctggcggcgc aggatccgct gaagtttgaa aaggacaaaa taaaaggggc
     5701 gattcgtacc gattttatat tgtctgctga aatcgtcgcc atcacgctgg ggattgtggc
     5761 cgaagcgccg ttgcttaatc aggtgctggt gctttcaggc atcgcgctgg tagtgaccgt
     5821 gggcgtctac ggtctggtag gggttatcgt taagattgat gacctggggt attggctggc
     5881 ggaaaaatcc agcgcgctga tgcaggcatt aggtaaaggg ttattgatca ttgcgccctg
     5941 gctgatgaaa gcgttatcga ttgtcggcac gctggcgatg ttcctcgtcg gcggcgggat
     6001 tgtggtacat ggtattgcgc cgctgcatca cgccattgaa catttcgccg ggcagcaaag
     6061 tgcagtggtg gcgatgatac taccgactgt tttaaatctg attcttggat ttatcatcgg
     6121 cggcatcgtg gtgctgggag tgaaagccgt agcgaaaata cgcggtcagg cacattaata
     6181 ctcgcacagg ctattatgca aaattcgcca tcttcaacta aggtgaacaa gtttcactcg
     6241 aaaaaaggag gcgagtatga gctttatggt tagtgaggaa gttacggtta aagagggcgg
     6301 cccgcggatg attgtcaccg gatactccag cggtatggtt gagtgtcgtt ggtatgacgg
     6361 ttacggggtc aagcgggaag cttttcatga aaccgagctt gttccggggg aggggagtcg
     6421 ttctgcggaa gaagtttgaa agggtatcat taacaccagg ccggatcact tccggcctgg
     6481 tgtgccaatg cctgcgatga atgatagtcg cgtcttatca tgccaccatt ataaaattat
     6541 cgctgttttg cggtacgcta tcgccaggtt atgccagaat cataaaaaag caggttggga
     6601 gtcgtcaggg tgcagcacga gacaaaaatg gaaaaccaga gctggttgaa aaaaatcgca
     6661 cgccgcctgg ggcctggtca tgtcgttaat ctctgcttta tcgtggtatt gcttttttcc
     6721 accttgctga cctggcgtga agtggtagtg ctggaagatg cctatatctc cagccagcgt
     6781 aatcatctgg aaaacgtagc caacgcgctc gataagcatt tgcagtgtaa cgtcgacaaa
     6841 ctgatctttt tgcgtaatgg catgcgcgaa gctctcgtag cgccactgga tttcacttca
     6901 ctgcgtaatg ctgtaaccga gttcgaacag catcgcgacg agcacgcctg gcaaattgaa
     6961 ctcaaccgac gacgcaccct gtcagtcaat ggcgtatcgg atgcattagt cagcgagggg
     7021 aatctcctgt ctcgcgaaaa tgaaagcctc gacaatgaaa ttaccgctgc actggaagtt
     7081 ggttacttgc tgcgactggc gcacaacacc tcgtcgatgg ttgaacaggc gatgtatgtc
     7141 tcgcgtgccg gattttacgt ttcgacgcag ccgaccttgt ttacgcgcaa tgtaccaacg
     7201 cgttattacg gctatgtcac ccaaccctgg tttatcggcc attcgcaacg agaaaatcgt
     7261 caccgcgcgg tacgctggtt tacttcgcaa ccggaacacg ccagcaatac tgaaccgcag
     7321 gttaccgtca gtgttccggt agacagtaat aactactggt atggcgtgct ggggatgagt
     7381 attcccgtgc gtaccatgca gcaattttta agaaacgcca tcgataaaaa cctcgatggt
     7441 gagtatcagc tctatgacag taagctgaga tttttgacct cttccaatcc tgaccatcca
     7501 acagggaata tttttgatcc tcgtgaactg gccttgctgg cgcaggcgat ggaacatgac
     7561 acgcggggcg gcattcgtat ggacagtcgc tatgttagct gggaacgtct ggaccatttc
     7621 gacggtgtgc tggtgcgcgt ccatacgcta agcgaaggcg tgcgcggcga tttcggcagt
     7681 atcagcattg cattaaccct gctgtgggcg ctctttacca ccatgttact catctcctgg
     7741 tatgtgattc gccggatggt tagcaacatg tatgttctgc aaagctcgtt gcagtggcag
     7801 gcgtggcacg acaccttaac ccgtttatat aatcgtggcg cactgtttga aaaagcccgt
     7861 ccgctcgcga aattgtgtca gacgcaccaa catccttttt ctgtcattca ggtcgacctt
     7921 gaccatttta aagcgattaa tgaccgcttt agtcatcagg cgggcgaccg tgttctttct
     7981 catgctgccg gattaattag cagttccttg cgtgcgcagg acgttgccgg gcgggtcggt
     8041 ggtgaggagt tttgtgtgat tctgccaggc gcgagtctga cggaggctgc ggaagtcgca
     8101 gaacgtattc gcctgaagtt aaatgaaaaa gagatgttga ttgctaagag tacgacgata
     8161 cgcatcagtg cctcgttggg ggtaagtagc agcgaggaaa ccggtgatta tgattttgaa
     8221 caactccagt cactggctga ccgtcggctt tatctcgcta aacaggccgg gcgtaatcgg
     8281 gtatgcgcga gcgataacgc ttaacgagcg gagaaaaaat ggtccagccc ttcacgccat
     8341 ccttccggtc cttcacgctg cgttcgccag acgcgggccg gatcctcatc atgcagatgc
     8401 accccttcac ggtttagccc tttcacaatc accgcgtaat ccattacctc cagtaagggc
     8461 gcatcgtttg gcccatcgcc caggccaagt gtggttgggc gtttgcctga taattgttga
     8521 taggtcgcga taatccagtt ggcggcctga tcttttccgg cagaggcatc caggacgtgc
     8581 cagaagcgcg caccctgcat aaactgtaag cccagttcgt tcagacgagc ggtaaattgt
     8641 gccatacgct cgtcactgtc gcgccagatt agcgttaccg acgcctcatg cagctgcgtc
     8701 agcgccgcct ggctacggct taatcccgtc cattcggcga tggttgcatc gtcgacatca
     8761 tcgaaagtcg tgaatttaaa atgttctttc tcgcgtagcg tatttaaaac ctggctgatt
     8821 tcgccatggc taatacctga gatgatgcgt ggaaaaccgt ctatatcctg ccattgttca
     8881 gcaagctgga tcactgcgcc attttcggca atcagtggta ccccttgtaa ccccaacgtt
     8941 ttttgcaagt acagcatttc cgctgaagtt ttactgctac agagaatgac gggaacattc
     9001 gcttcgcgta aacggctgag ccagggggca gccggttgcc agtcataact atgactgtcc
     9061 agcagggtgc catcaagatc gctaaaaacc agtagtggtt gttgaattga aaacatggtc
     9121 gcgcagttct ccccttacca ggatgattct caaaacagca caaataatca aagatgattt
     9181 tttcgggagc gatcccgttg acgccagagc caccaagggg tgacgcacgt cacatttcta
     9241 ttcataagta gcgttaatca ttcatatcgc gaatattttc ttgtcagcga aaaaaattgc
     9301 ggataaggtg atgaacacat cagatttcct ggtgtaacga attttttaag tgcttcttgc
     9361 ttaagcaagt ttcatcccga ccccctcagg gtcgggattt ttttattgtg cattcaacga
     9421 ttcacttcag agacgctgaa gtcatgaata tccagttcga aggcatccgc caattcacgc
     9481 catgtatgat attcacgtcc atcgacgcta acgtgttcag aatcgttctg gctgcgatga
     9541 acttcgcttt cgctaatttc attgatcgcc gccagcaggg catcgacatc gacgctgacc
     9601 tcacgttttg cggtatcgct gtactctttt gcggttttca tcatctttcc tcgcaaccgt
     9661 tactctgttg ttgccgccag tcgttaagtc cgcctggcat ctcttaagta tagaccgtcg
     9721 agaaaatcag cactcgcgcg cgcctggcgc tgccagaaaa acgaaattgt tctacactgg
     9781 cacaaagcca caggaggaaa acgatgaagg tgaatgatcg ggtaacagtc aaaacggatg
     9841 gcggtccgcg tcgtcctggc gtggtactag cagttgagga gtttagtgaa ggcacaatgt
     9901 acctggtttc gctggaagac tacccgctcg gcatctggtt ctttaatgaa gcagggcatc
     9961 aggacggtat ctttgtggag aaagcagagt aatcttattc agcctgactg gtgggaaacc
    10021 accagtcaga atgtgttagc gcatgttgac aaaaatacca ttagtcacat tatccgtcag
    10081 tcggacgaca tggtagataa cctgtttatt atgcgttttg atcttacgtt taatattacc
    10141 tttatgcgat gaaacggtct tggctttgat attcatttgg tcagagattt gaatggttcc
    10201 ctgacctgcc atccacattc gcaacatact cgattcggtt cggctcaatg ataacgtcgg
    10261 catatttaaa aaagaggtta tcgttgtctc ttttttcaga atatcgccaa ggatatcgtc
    10321 gagagattcc ggtttaatcg atttagaact tatcaataaa atttttctga ccaatagata
    10381 ttcatcaaaa tgaacattgg caattgccat aaaaacgata aataacgtat tgggatgttg
    10441 attaatgatg tgcttgatgt gctgactgtt agaagcatca tggatgaaac agtcctcatt
    10501 aataaacacc actgaagggc gctgtgaatc acaagctatg gcaaggtcat caacggtttc
    10561 aatgtcgttg atttctcttt ttttaacccc tctactcaac agatacccgg ttaaacctag
    10621 tcgggtgtaa ctacataaat ccataataat cgttg
//
LOCUS       DAJTNA010000135        10575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTNA010000135 DAJTNA010000000
VERSION     DAJTNA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.136"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(39..494)
                     /locus_tag="OO161_002996"
     CDS             complement(39..494)
                     /locus_tag="OO161_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5463985.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            832..2034
                     /gene="ackA"
                     /locus_tag="OO161_002997"
     CDS             832..2034
                     /gene="ackA"
                     /locus_tag="OO161_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5463986.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            2109..4253
                     /gene="pta"
                     /locus_tag="OO161_002998"
     CDS             2109..4253
                     /gene="pta"
                     /locus_tag="OO161_002998"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5463987.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYAEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPNPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            4443..5963
                     /gene="yfcC"
                     /locus_tag="OO161_002999"
     CDS             4443..5963
                     /gene="yfcC"
                     /locus_tag="OO161_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5463988.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRADFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(5996..6538)
                     /gene="yfcD"
                     /locus_tag="OO161_003000"
     CDS             complement(5996..6538)
                     /gene="yfcD"
                     /locus_tag="OO161_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5463989.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(6596..7147)
                     /gene="yfcE"
                     /locus_tag="OO161_003001"
     CDS             complement(6596..7147)
                     /gene="yfcE"
                     /locus_tag="OO161_003001"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5463990.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQKDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(7203..7847)
                     /gene="yfcF"
                     /locus_tag="OO161_003002"
     CDS             complement(7203..7847)
                     /gene="yfcF"
                     /locus_tag="OO161_003002"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5463991.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSVIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            7983..8630
                     /gene="yfcG"
                     /locus_tag="OO161_003003"
     CDS             7983..8630
                     /gene="yfcG"
                     /locus_tag="OO161_003003"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5463992.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDQGKGDQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQARLKAQLGDERSDS"
     gene            8687..9049
                     /gene="folX"
                     /locus_tag="OO161_003004"
     CDS             8687..9049
                     /gene="folX"
                     /locus_tag="OO161_003004"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5463993.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            9070..9963
                     /locus_tag="OO161_003005"
     CDS             9070..9963
                     /locus_tag="OO161_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5463994.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(10011..>10575)
                     /gene="rpnB"
                     /locus_tag="OO161_003006"
     CDS             complement(10011..>10575)
                     /gene="rpnB"
                     /locus_tag="OO161_003006"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5463995.1"
                     /translation="GYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSA
                     FPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKA
                     LFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQE
                     MLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         2515 a         2787 c         2655 g         2618 t
ORIGIN      
        1 tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt acaaatcatc aaggaaagtt
       61 ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta atcaggcttg
      121 ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt taccgcgaac ttcataaaac
      181 cagttgagga tagcaggggg taatggcgtt acagaacgct tgcccagcca ccataatccc
      241 tgcatgggta aacttaaggc gaacagcgca gtggcaactg ccggcccaag ctgaccgccc
      301 agggcaatct gccagcagag agtaaatacg gcgatcggcg gcataaaacg gatcgcataa
      361 cgcgtcatct tgataacgcg attttcgaca aagaccgggg caaggcgttt ttccagcggc
      421 cacgtctttg agtaatgctg tccccggcga aacaagctaa aaaaattaac agaacgatta
      481 tccggcgttg acatgcttca cctcaacttc acatataaag attcaaaaat ttgtgcaaat
      541 tcacaactca gcgggacaac attcaaaaca ttttgtcttc catacccact atcaggtatc
      601 ctttagcagc ctgaaggcct aagtagtaca tattcattga gtcgtcaaat tcatatacat
      661 tatgccattg gctgaaaatt acgcaaaatg gcatagactc aagatatttc ttccatcatg
      721 caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca tgcgctacgc
      781 tctatggctc cctgacgttt ttttagccac gtatcaatta taggtacttc catgtcgagt
      841 aagttagtac tggttctgaa ctgcggtagt tcttcactga aatttgccat catcgatgca
      901 gtaaatggtg aagagtacct ttctggttta gccgaatgtt tccacctgcc cgaagcacgt
      961 atcaaatgga aaatggacgg caataaacag gaagcggctt taggtgcagg cgccgctcac
     1021 agcgaagcgc tcaactttat cgttaatact attctggcac aaaaaccaga actgtctgcg
     1081 cagctgactg ctatcggtca ccgtatcgta cacggcggcg aaaagtatac cagctccgta
     1141 gtgatcgatg agtctgttat tcagggtatc aaagatgcag cttcttttgc accgctacac
     1201 aacccggctc acctgatcgg tatcgaagaa gctctgaaat ctttcccaca gctgaaagac
     1261 aaaaacgttg ctgtatttga caccgcgttc caccagacta tgccggaaga gtcttacctc
     1321 tacgccctgc cttacaacct gtacaaagag cacggcatcc gtcgttacgg cgcgcacggc
     1381 accagccact tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc ggtagaagaa
     1441 ctgaacatca tcacctgcca cctgggcaac ggtggttccg tttctgctat ccgcaacggt
     1501 aaatgcgttg acacctctat gggcctgacc ccgctggaag gtctggtcat gggtacccgt
     1561 tctggtgata tcgatccggc gatcatcttc cacctgcacg acaccctggg catgagcgtt
     1621 gacgcaatca acaaactgct gaccaaagag tctggcctgc tgggtctgac cgaagtgacc
     1681 agcgactgcc gctatgttga agacaactac gcgacgaaag aagacgcgaa gcgcgcaatg
     1741 gacgtttact gccaccgcct ggcgaaatac atcggtgcct acactgcgct gatggatggt
     1801 cgtctggacg ctgttgtatt cactggtggt atcggtgaaa atgccgcgat ggttcgtgaa
     1861 ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg atcatgaacg caacctggct
     1921 gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc ggtggttatc
     1981 ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc ctgatttcac
     2041 accgccagct cagctggcgg tgctgttttg taacccgcca aatcggcggt aacgaaagag
     2101 gataaaccgt gtcccgtatt attatgctga tccctaccgg aaccagcgtc ggtctgacca
     2161 gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg agcgttttca
     2221 aacctatcgc tcagccgcgt accggtggcg atgcgcccga tcagactacg actatcgtgc
     2281 gtgcgaactc ttccaccacg acggccgctg aaccgctgaa aatgagctac gctgaaggtc
     2341 tgctttccag caatcagaaa gatgtgctga tggaagagat cgtcgcaaac taccacgcta
     2401 acaccaaaga cgctgaagtc gttctggttg aaggtctggt cccgacacgt aagcaccagt
     2461 ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc gtcttcgtta
     2521 tgtctcaggg cactgacacc ccggaacagc tgaaagagcg tatcgaactg acccgcaaca
     2581 gcttcggcgg tgccaaaaac accaacatca ccggcgttat cgttaacaaa ctgaacgcac
     2641 cggttgatga acagggtcgt actcgcccgg atctgtccga gattttcgac gactcttcca
     2701 aagctaaagt aaacaatgtt gatccggcga agctgcaaga atccagcccg ctgccggttc
     2761 tcggcgctgt gccgtggagc tttgacctga tcgcgactcg tgcgatcgat atggctcgcc
     2821 acctgaatgc gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt aaatccgtca
     2881 ctttctgcgc acgcagcatt ccgcacatgc tggagcactt ccgtgccggt tctctgctgg
     2941 tgacttccgc agaccgtcct gacgtgctgg tcgccgcttg cctggcagcc atgaacggcg
     3001 tagaaatcgg tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc atttctaaac
     3061 tgtgcgaacg tgctttcgct accgggctgc cggtatttat ggtgaacacc aacacctggc
     3121 agacctctct gagcctgcag agcttcaacc tggaagttcc ggttgacgat cacgaacgta
     3181 tcgagaaagt tcaggaatac gttgctaact acatcaacgc tgactggatc gaatctctga
     3241 ctgccacttc tgagcgcagc cgtcgtctgt ctccgcctgc gttccgttat cagctgactg
     3301 aacttgcgcg caaagcgggc aaacgtatcg tactgccgga aggtgacgaa ccgcgtaccg
     3361 ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg ctgggtaatc
     3421 cggcagagat caaccgtgtt gcagcgtctc agggtgtaga actgggtgca gggattgaaa
     3481 tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg tctggtcgaa ctgcgtaaga
     3541 acaaaggcat gaccgaaacc gttgcccgcg aacagctgga agacaacgtg gtgctcggta
     3601 cgctgatgct ggaacaggat gaagttgatg gtctggtttc cggtgctgtt cacactaccg
     3661 caaacaccat ccgtccgccg ctgcagctga tcaaaactgc accgggcagc tccctggtat
     3721 cttccgtgtt cttcatgctg ctgccggaac aggtttacgt ttacggtgac tgtgcgatca
     3781 acccgaatcc gaccgctgaa cagctggcag aaatcgcgat tcagtccgct gattccgctg
     3841 cggccttcgg tatcgaaccg cgcgttgcta tgctctccta ctccaccggt acttctggtg
     3901 caggtagcga cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa aaacgtcctg
     3961 acctgatgat cgacggtccg ctgcagtacg acgctgcggt aatggctgac gttgcgaaat
     4021 ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt gtttatcttc ccggatctga
     4081 acaccggtaa caccacctac aaagcggtac agcgttctgc cgacctgatc tccatcgggc
     4141 cgatgctgca gggtatgcgc aagccggtta acgacctgtc ccgtggcgca ctggttgacg
     4201 atatcgtcta caccatcgcg ctgactgcga ttcagtctgc acagcagcag taatctcgtc
     4261 atcatccgca gctttgcgct gcggatatct gaaccggaaa taatcactat ttccggtttt
     4321 ttattctctt aatctgcatt aatcctttct gattatcttg cttaactgcg ctgcatcaat
     4381 gaattgcgcc atttcacttt gcatacttac cactttgttt tgtgcaaggg aatatttgcg
     4441 ctatgtccgc aatcactgaa tccaaaccaa caagaagatg ggcaatgccc gatacgttgg
     4501 tgattatctt ttttgttgct attttaacca gccttgccac ctgggtagtt ccggtgggga
     4561 tgtttgacag tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca cgcaaagtcg
     4621 tagatccaca ctcatttcgc attctgacta acgaagcagg cgaacctgag tatcaccgcg
     4681 tacagctgtt cacgacgggc gatgaacgcc cgggcctgat gaacttcccg tttgaaggat
     4741 taacctcagg atcgaaatac gggacagctg ttggcatcat catgtttatg ctggtgattg
     4801 gcggcgcgtt tggcattgtg atgcgtacag gaaccattga taacggtatc ctggcgctta
     4861 ttcgccatac ccgcgggaat gaaattctct ttattcctgc gctgtttatt ctgttttcac
     4921 ttggcggcgc ggtatttggt atgggagaag aggccgtcgc ctttgccatt atcatcgcac
     4981 cgctaatggt ccggctgggc tatgacagta ttaccaccgt cctggtgacc tatattgcca
     5041 cgcaaatcgg ttttgccagt tcgtggatga acccgttttg tgtggtcgtt gctcagggga
     5101 ttgccggcgt tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg gttatcgcca
     5161 ctatgattgg cctgatcttt accatggtgt acgcctcacg agtgaaaaag aatcctcttc
     5221 tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa gcaggcggat gttgaacaac
     5281 gtccgtttac ctttggtgac tggctggtat tgattgtcct gaccgccgta atggtctggg
     5341 tgatttgggg cgtgatcgtt aatgcctggt ttattccaga aattgccagc cagttcttca
     5401 ccatgggtct ggtgattggc atcatcggcg ttgttttccg ccttaacggc atgacggtta
     5461 ataccatggc ttcatccttt accgaagggg cgcgaatgat gatcgcccct gccctgctgg
     5521 tgggtttcgc caaagggatt ttgctgctgg tcggtaatgg tgaagcgggt gatgccagcg
     5581 tgttaaatac catcctcaac agcattgcca atgccattag cggtctggac aacgcggtcg
     5641 cggcctggtt tatgttgctc ttccaggcgg tatttaattt cttcgtgacg tccggttctg
     5701 gtcaggcggc gttaaccatg ccgttactgg caccgcttgg cgatctggtc ggtgttaacc
     5761 gtcaggttac cgtgctggca ttccagtttg gtgatggctt cagtcacatc atttacccaa
     5821 cctcagcttc gttaatggca actctcggtg tgtgcagggc ggacttccgt aactggctga
     5881 aggtgggcgc gaccctactt ggactgctgt ttattatgtc cagcgtcgta gtgatcggcg
     5941 ctcagttgat gggctaccac taaaaatgtt aagagccgca attgcggctc ttttttcatt
     6001 ctgccgtttc agtctctacg gcttcatttt tggcattgcg cttcatccac aacgccagcg
     6061 ctttcagcga gtctggagtg aactcatcgc agcgtgcggt gatttcttcc ggcgtcagcc
     6121 agcaaacttc actgacttca tcttcctgta gggcgaaggg accgtgagag acgcagctga
     6181 acaacgcgcc ccagacacgg caatttttat cttcgaaata gaactgcccg tgctcggcaa
     6241 aggggacacc ggcaatgccc aactcttctt ccgcttcgcg acgcgcggat tccagcagtt
     6301 gctcatcggc ctgaaccaca ccgcctgcgg tcgcatctaa catgccgggt aaaaagtctt
     6361 ttgtctcggt acgacgctgg accagaattt tgcccatgcc atcatgcacg acgatgtaag
     6421 ttgcacgatg acgcagacac tgtgcccgca tttgttcccg gctggcttgt gcaatgactt
     6481 cgttctcttc attgacaata tccacccatt cagtacttgc caaacgacgc tgttccatta
     6541 tcaggaaacc ttctttttct ggcgctctta cggcgcattt tgagttgtgg gtaaattacg
     6601 gattaatcgc gacctgcgca atgatacttt gatcattgag tgcgattaca ctaagtacgt
     6661 cattatccag catgccataa ctcgccggat taccgccttt cggaatactc accgagccgg
     6721 ggttgaagtg gaaaatctcc ccccgctgtt ctgccactgg cagatgggtg tgaccgtaca
     6781 ccagcacatc cttctggttt aaagcaggta gattttccgg gccaaaaaga tgaccatgcg
     6841 tcaaaaacag acgttgtttt tccagtaata cctgttgcca cggcgcggtt atcgggaaat
     6901 gcagcagcat ttgatccact tcgctgtcgc agttgccgcg cacagcgata accttatgtg
     6961 ccacttcatt aagccgttca gcgactttgg ctggcgcgta accctccggt aaagcattac
     7021 gcgggccgtg attcaacacg tcgccaagga tcaccagcca ctgggcaccg ctttgggcaa
     7081 acaactccag aacacgttcc gtcgccggta acgacccatg aatgtccgat gcaaacatca
     7141 gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg atactggatt ctgtcgttgc
     7201 tatcagccag attgcttcgc cgaaagtgca ataaaacgct ggacagacgc tcgctgccac
     7261 tggaatgtcg cataatccac caggcgttct ggcacctcat cgccatgtag taccaggcgg
     7321 ttaatcatta gcgccagatc agtatcagca atgcaccatt caccaaataa attcggctga
     7381 cccagtgcta acaaatgttc tgccatcgcg aacagtttct ctgcactggc ttttccctcg
     7441 gccgttagtg gcgctttttt cgcccccgca aagacaacat ccgtcggacg ctcttcgcgg
     7501 atgggcatca gatcgctgcg cagccaggcc tgaatctgtc gtgcacgcgc acgattttct
     7561 aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc cagatactcc
     7621 gcaatgacag aagattcact caactcaaaa tcatcgattt gtaataacgg cacacgacgt
     7681 gtctgaccgt aaccttgcca cgtcggctgc aaatgttcac cgctgtcgag gtcgatggtc
     7741 ttgatatgaa acgacaggcc tttttcctgc aacgccaccc aagcggataa cacataaggg
     7801 gagaaaaagt gggcatctga ccaaagcgtg atagcgggtt tactcattat gtcctcggca
     7861 ataaaggggg ctggctatca aactatagag cctttttgtc actgtcacgt gacgaatact
     7921 cacgtaagtc gccgcgcttt ctatacttgt ggtgttcaga tgattaaaca accggagctg
     7981 caatgatcga tctctatttc gccccgacac ccaatggtca caaaattacg ctgtttctcg
     8041 aagaagcaga gcttgattat cgcttgatta aggtagacca ggggaaaggc gatcagtttc
     8101 gtccggaatt tttgcgcatt tcgcccaaca acaaaattcc ggcaattgtt gatcattctc
     8161 ctgccgatgg cggcgaaccg ctaagcctct ttgaatctgg tgccattttg ttgtatctgg
     8221 ctgagaaaac ggggctcttt ttgagtcatg aaacgcgtga acgcgccgcc acattacagt
     8281 ggttattctg gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat cattttaatc
     8341 acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa actcagcgtc
     8401 tttaccatgt actaaacaag cggctggaaa actcgccctg gctgggaggc gagaactaca
     8461 gcattgcgga tattgcctgc tggccgtggg ttaatgcctg gactcgccag cgaattgacc
     8521 tcgcaatgta tccggcagtc aagaactggc atgagcggat ccgttcgcgc cctgccaccg
     8581 ggcaggcacg gctaaaagca caactcggtg atgagcgttc ggatagttaa cagaaacagg
     8641 ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg cacaacctgc
     8701 cgctattatt cgtataaaga accttcgttt acgtacgttt atcggaatta aggaagaaga
     8761 aattaacaac cgtcaggata ttgttatcaa tgtgacgatc cactaccccg ccgataaagc
     8821 gcgcactagc gaagatatca acgatgcgct gaattatcgc accgtaacga aaaacattat
     8881 tcagcatgta gagaataacc gtttctcttt gttggaaaaa ttaactcagg atgtgctcga
     8941 tatcgcacgt gaacatcact gggtgacgta tgctgaagtg gagatcgata aactgcacgc
     9001 gctgcgctac gccgattcgg tatccatgac cttaagctgg cagcgttaac cgccatatcg
     9061 ggaggctgca tgaacatagt gatcaccgga gggacgggat taattggtcg ccatttgatt
     9121 ccgcgtttgc tggagctggg ccatcaaatc acggtagtga cgcgtaaccc acagaaagcc
     9181 agttccgttc tcggccctcg ggtgacgcta tggcaagggc tagccgatca aagcaacctc
     9241 aacggcgttg atgcggtaat caacctggcc ggagaaccga ttgctgataa acgctggact
     9301 cacgagcaaa aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa actggtcgat
     9361 ttgattaatg ccagcgacac gccgccgtcg gtactcattt ccggctcggc aacgggctat
     9421 tatggcgact taggtgaagt ggtggttacc gaagaggaac cgccgcataa cgaatttact
     9481 cataaactct gcgcccgctg ggaagagatt gcctgccggg cgcaaagtga caaaacgcga
     9541 gtgtgcctgc tgcgaaccgg cgtggtactg gcaccggacg gcggtattct cggtaaaatg
     9601 ctgccaccgt ttcgtcttgg cctgggcggg ccgattgact ctggtcggca gtatctggcc
     9661 tggattcata ttgatgatat ggtcaacggt attctctggc tgctggataa cgagctgcgc
     9721 gggccattta atatggtttc gccctacccg gtacgcaatg aacaatttgc ccatgcgctc
     9781 ggtcacgcgc tgcatcgccc ggccattttg cgcgtccccg cgaccgccat tcgactgtta
     9841 atgggcgaat cttcagtact ggtattaggt ggacaacgcg cgctgcctaa acggctggaa
     9901 gaagcgggtt ttgcgtttcg ctggtacgat ttagaagagg cgctggcgga tgtcgttcgc
     9961 tgatgtggtt tacagcaaac atccgccagt taactcccgg tgttacagga ttagtggctt
    10021 tgcgcgataa gatcgtctgg tgaaagtcgg gtcaccatca taactaactc tctgtctaaa
    10081 cctctatcca gcatctcctg agcaatacgc agggcttctt cgtgtttgcc ctgcattgcg
    10141 ccttcttcac gtaatctgtc agcaatggtc atcagtttct ccttttcttg tggtgcgcgt
    10201 tccgctatct caccaataaa tgcacgaaaa cgctgggcat cccctgtttg taatacgtaa
    10261 ttaaacaggg cttttagctg tctgtcatta gtgttccctg taactagcag cgaaacaatt
    10321 tggtcgacta atcccaacag atcgcgctga cgaatatgtt tctgaattaa ctccaacagc
    10381 gccattttgc ggtgttgcat aatctcgtca tccggcacca cggtaatatc caccaacgga
    10441 aacgctgatg aatatatttt gcgggctata gcaggctcgg caaattcatc aagccagcag
    10501 agtgaataag gataaggact tctgcaacca tgataaaaca gcatcgggat caccaatgga
    10561 agctctttat agccc
//
LOCUS       DAJTNA010000136        10544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTNA010000136 DAJTNA010000000
VERSION     DAJTNA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.137"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..450
                     /locus_tag="OO161_003007"
     CDS             <1..450
                     /locus_tag="OO161_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5463996.1"
                     /translation="ALNKFTGGISQLDDAAEQKCILESIQQLKVKTSSPDLAIGRLSG
                     GNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQLVQQGIAVIVISSELP
                     EVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHVEKQSV"
     gene            428..1609
                     /gene="xylH"
                     /locus_tag="OO161_003008"
     CDS             428..1609
                     /gene="xylH"
                     /locus_tag="OO161_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5463997.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            1687..2865
                     /gene="xylR"
                     /locus_tag="OO161_003009"
     CDS             1687..2865
                     /gene="xylR"
                     /locus_tag="OO161_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCR5463998.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(2973..3797)
                     /locus_tag="OO161_003010"
     CDS             complement(2973..3797)
                     /locus_tag="OO161_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5463999.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            4117..6147
                     /gene="malS"
                     /locus_tag="OO161_003011"
     CDS             4117..6147
                     /gene="malS"
                     /locus_tag="OO161_003011"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5464000.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSTPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLIDSAHQRGIRILFDVVMNHTGYATLVDMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPEKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVVHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            6325..7578
                     /gene="avtA"
                     /locus_tag="OO161_003012"
     CDS             6325..7578
                     /gene="avtA"
                     /locus_tag="OO161_003012"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5464001.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(7729..8202)
                     /locus_tag="OO161_003013"
     CDS             complement(7729..8202)
                     /locus_tag="OO161_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5464002.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPPCVEACPTKALQFMDVEKVQRHRLRQQPV"
     gene            complement(8304..9152)
                     /gene="yiaJ"
                     /locus_tag="OO161_003014"
     CDS             complement(8304..9152)
                     /gene="yiaJ"
                     /locus_tag="OO161_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5464003.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIMH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            9353..10345
                     /gene="yiaK"
                     /locus_tag="OO161_003015"
     CDS             9353..10345
                     /gene="yiaK"
                     /locus_tag="OO161_003015"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5464004.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHCIGLVALRNANHWMRGGSYGWQTAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            10357..>10544
                     /locus_tag="OO161_003016"
     CDS             10357..>10544
                     /locus_tag="OO161_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5464005.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQIIDLTTREAVENRP"
BASE COUNT         2605 a         2700 c         2647 g         2592 t
ORIGIN      
        1 gcactcaata aatttaccgg tggcattagc cagcttgatg acgcggcaga gcaaaaatgt
       61 attctggaat caatccagca actcaaagtt aaaacgtcgt cccccgatct tgctattgga
      121 cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc gctgtctgtt actcaacccg
      181 cgcattctca ttcttgatga acctaccagg ggtattgata ttggcgcgaa atatgagatc
      241 tacaaattaa ttaaccaact cgtccagcag ggtattgccg ttattgtcat ctcttccgaa
      301 ttacctgaag tgctcggcct tagcgatcgt gtactggtga tgcatgaagg gaaactaaaa
      361 gccaacctga taaatcataa cctgactcag gagcaggtga tggaagccgc attgaggagc
      421 gaacatcatg tcgaaaagca atccgtctga agtgaaattg gccgtaccga catccggtgg
      481 cttttccggg ctgaaatcac tgaatttgca ggtcttcgtg atgattgcag ctatcatcgc
      541 aatcatgctg ttctttacct ggaccaccga tggtgcctac ttaagcgccc gtaacgtctc
      601 caacctgtta cgccagaccg cgattaccgg catcctcgcg gtaggaatgg tgttcgtcat
      661 aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg gggctattag gtggcgtcgc
      721 ggcgatttgt gacgtctggt taggctggcc tttgccactt accatcattg tgacgttggt
      781 tctgggactg cttctcggtg cgtggaacgg atggtgggtc gcgtaccgta aagtcccttc
      841 atttattgtc accctcgcgg gcatgttggc atttcgcggc atactcattg gcatcaccaa
      901 cggcacgact gtatccccca ccagcgccgc gatgtcacaa attgggcaaa gctatctccc
      961 cgccagtacc ggcttcatca ttggcgcgct tggcttaatg gcttttgttg gttggcaatg
     1021 gcgcggaaga atgcgccgtc aggctttggg tttacagtct ccggcctcta ccgcagtagt
     1081 cggtcgccag gctttaaccg ctatcatcgt attaggcgca atctggctgt tgaatgatta
     1141 ccgtggcgtt cccactcctg ttctgctgct gacgttgctg ttactcggcg gaatgtttat
     1201 ggcaacgcgg acggcatttg gacgacgcat ttatgccatc ggcggcaatc tggaagcagc
     1261 acgtctctcc gggattaacg ttgaacgcac caaacttgcc gtgttcgcga ttaacggatt
     1321 aatggtagcc atcgccggat taatccttag ttctcgactt ggcgctggtt caccttctgc
     1381 gggaaatatc gccgaactgg acgctattgc agcatgcgtg attggcggca ccagcctggc
     1441 tggcggtgtg gggagcgttg ccggagcagt aatgggggca tttatcatgg cttcactgga
     1501 taacggcatg agtatgatgg atgtaccgac cttctggcag tatatcgtta aaggtgcgat
     1561 tctgttgctg gcagtatgga tggactccgc aaccaaacgc cgttcttgat tttgataaaa
     1621 attttctcaa agccggttac gtattaccgg ttttgagttt ttgcatgatt cagcaggaaa
     1681 agaaccatgt ttactaaacg tcaccgcatc acattactgt tcaatgccaa taaagcctat
     1741 gaccggcagg tagtagaagg cgtaggggaa tatttacagg cgtcacaatc agaatgggat
     1801 attttcattg aagaagattt ccgcgcccgc attgataaaa tcaaggactg gttaggagat
     1861 ggcgtcattg ccgacttcga cgacaaacaa atcgagcaag cgctggctga tgtcgacgtc
     1921 cccattgttg gggttggcgg ctcgtatcac cttgcagaaa gttacccacc cgttcattac
     1981 attgccaccg ataactatgc gctggttgaa agcgcatttt tgcatttaaa agagaaaggc
     2041 gttaaccgct ttgcttttta tggtcttccg gaatcaagcg gcaaacgttg ggccactgaa
     2101 cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt atcgcggagt ggtttatcag
     2161 gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa atcggctggc agactggcta
     2221 caaacgctgc caccgcaaac cgggattatt gccgttactg acgcccgggc gcggcatatt
     2281 ctgcaagtat gtgaacatct acacattccc gtaccggaaa aattatgcgt gattggcatc
     2341 gacaacgaag aactgacccg ctatctgtcg cgtgtcgccc tttcttcggt cgctcagggc
     2401 gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc gattattaga taaagaagaa
     2461 atgccgctac agcgaatttt ggtcccacca gttcgcgtca ttgaacggcg ctcaacagat
     2521 tatcgctcgc tgaccgatcc cgccgttatt caggccatgc attacattcg taatcacgcc
     2581 tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg ggatctcgcg ctccaatctt
     2641 gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg ccatgattca tgccgagaag
     2701 ctggagaaag cgcgcagtct gctgatttca accaccttgt cgatcaatga gatatcgcaa
     2761 atgtgcggtt atccatcgct gcaatatttc tactctgttt ttaaaaaagc atatgacaca
     2821 acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt tgtagcgatg atgagaattg
     2881 tcggcgtcac atcagggaac tcagcacagc cacctgatgc gagcaaaagc cttatccgac
     2941 cgacaaatca gataaggctc aaggaaatgc aattacatat gcgccgcgat taaccgttgg
     3001 ttatcctggt acattgcgaa caggtagtta ttataactct tccctttggt cgagtagccc
     3061 ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat ccgctttacg cagctgcgca
     3121 cgcgatttac ggaacgaaga gtaagccggg tgcgtattca ggttagtgac ataggcgctc
     3181 accgattctt tgacagaact aaactgtgag taccctttca ctttacccgg cgcattggta
     3241 caacgtcctt tcatgcattt catgccgaac agattgttat tgttgcgcgc cagcttcgac
     3301 gttccccaac cgctttctgc tgcagccatc gtcgccacca tactggtagg gataatgtct
     3361 acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat taccagacca cttcaccttg
     3421 tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag gtgaccattg gccctgatac
     3481 tgttttgaaa tcagccagtt acgttctgca gtaatggctg cattttggct ggtaatgtaa
     3541 ggcattacgg tccggagaaa cgcctttttc cttggtgttc cggaagggta ttttcgcaaa
     3601 tcaggaagtg aactactctt tgcactattg cgagaatact cttgtttact gcttacctgt
     3661 ttattactag ctttagttaa gtgggacttt tgactcgctg ttgttgtgtg cgtcttcgct
     3721 aacacctcac tcgaaaacac cagagtgagt aacataagaa tcatcgcccc atatcgtcgt
     3781 atgggagtca aaatcatcag gtctcctggt cggatttaat cattccaaca ccttatattt
     3841 ttcacaaatt tgagagttga atctcaaatc atatcaaaaa tagctgtcaa gagcacccca
     3901 aggaatagtc caaatctgaa actatgtcac gagttaacga ttcagattgg cgctaaatcg
     3961 cagaaaatgt ggggtttatc gcaaaattca gccgtttttt gcgcgcgatc gctcaccctt
     4021 gcttctcatc ctgtggactt accgctcagg gatgagtttt gtctggctta tcgctggcaa
     4081 actgtctgaa atcgcagcaa taaggactca tccaccatga aactcgcctc ctgttttctg
     4141 acactccttc ctggcttcgc cgttgccgcc agctggactt ctccggggtt ccctgccttt
     4201 agcgaacagg gaacgggaac atttgtcagc cacgcgcagt tgcccaaagg tacgcgtcca
     4261 ctcacgctaa attttgacca acagtgctgg cagcctgcag atgcgataaa actcaatcag
     4321 atgctttccc tgcaaccttg tagcaacacg ccgcctcaat ggcgattgtt cagggacggc
     4381 aaatatacgc tgcaaataga cacccgctcc ggtacgccaa cattgatgat ttccatccag
     4441 aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc cgaaatggga tggattaccg
     4501 ctcacgctgg atgtcagcgc cactttcccg gaaggagccg ccgtacggga ttattacagc
     4561 cagcaaattg cgatagtgaa gaacggtcaa ataacgttac aacccgctgc taccagcaac
     4621 ggtttactcc tgctggaacg ggcagaaact gacacatcca cccctttcga ctggcataac
     4681 gccacggttt actttgtgct gacagatcgt ttcgaaaacg gcgatcccag taatgaccag
     4741 agttacggac gtcataaaga cggtatggcg gaaattggca cttttcacgg cggcgattta
     4801 cgcggcctga tcaacaaact ggattacctc cagcagttgg gcgttaatgc tttatggata
     4861 agcgccccat ttgagcaaat tcacggctgg gtcggcggcg gtacaaaagg cgatttcccg
     4921 cattatgcct accacggtta ttacacacag gactggacga atcttgatgc caatatgggc
     4981 aacgaagccg atctacggac gctgattgat agcgcacatc agcgcggtat tcgtattctc
     5041 tttgatgtag tgatgaacca caccggctat gccacgctgg tggatatgca ggagtatcag
     5101 tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa cgctgggtga acgctggagc
     5161 gactggaaac ctgccgccgg gcaaacctgg catagcttta acgattacat taatttcagc
     5221 gacaaaacag gctgggataa atggtgggga aaaaactgga tcagaacgga tatcggcgat
     5281 tacgacaatc ctggattcga cgatctcact atgtcgctgg cctttttgcc ggatatcaaa
     5341 accgaatcaa ctaccgcttc tggtctgccg gtgttctata aaaacaaaac ggatactcac
     5401 gctaaagtca tcgaaggctt tacacctcgc gattacttaa cccactggtt aagtcagtgg
     5461 gtccgcgact atgggattga tggttttcgg gtcgataccg ccaaacatgt tgagttgccc
     5521 gcctggcagc aactgaaaac cgaagccagc tccgcgcttc gcgaatggaa aaaaactaac
     5581 cccgagaaag cattagatga caaacctttc tggatgaccg gtgaagcctg gggccacggc
     5641 gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga tgatcaattt cgattatcag
     5701 gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg atacgacctg gcagcaaatg
     5761 gcggagaaat tgcagggttt caacgtgttg agctacctct cgtcgcatga tacccgtctg
     5821 ttccgtgaag ggggcgacaa agcagcagag ttattactat tagcgccagg cgcggtacaa
     5881 atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta caggttctga tccgctgcaa
     5941 ggtacacgtt cggatatgaa ctggcaggat gttagcggta aatctgccgc caacgtcgtg
     6001 cactggcaga aaatcagcca gttccgcgcc cgccatcccg caattggcgc gggcaaacaa
     6061 acgacacttt cgctgaagca gggctacggc tttgttcgtg agcatggcga cgataaagtg
     6121 ctggtcatct gggctgggca acagtgactt ttccggcttc ccgttcatca gtacctcggg
     6181 aagccgccaa ccaggataaa atgtcagccc taatcagcgt tgcaggataa agcaccgctc
     6241 actcttcaac agaccgattt gcaccccagc aaatgtagcg ttattgttac cttccttgct
     6301 acagagttcg acagatatcc cgctatgaca ttctcccttt ttggtgacaa atttacccgc
     6361 cactccggca ttacgctgct gatggaagat ctgaacgacg gtttacgcac gcctggcgcg
     6421 attatgctcg gcggcggtaa tccggcgcag atcccggaaa tgcaggacta cttccagacg
     6481 ctactgaccg acatgctgga aagtggcaaa gcgactgatg cactgtgtaa ctacgacggt
     6541 ccacagggga aaacggagct actcacactg cttgccggaa tgctgcgcga gaagttgggt
     6601 tgggatatcg aaccacagaa tattgcacta acaaacggca gccagagcgc gtttttctac
     6661 ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg tcaaaaaagt gctgttcccg
     6721 ctggcaccgg aatacattgg ctacgctgac gccggactgg aagaagatct gtttgtctct
     6781 gcgcgtccga atattgaact gctgccggaa ggccagttta aataccacgt cgattttgag
     6841 catctgcata ttggcgaaga aaccgggatg atttgcgtct cccggccgac gaatccaaca
     6901 ggcaatgtga ttactgacga agagttgctg aagcttgacg cgctggcgaa tcaacacggc
     6961 attccgctgg taattgataa cgcttatggc gtcccgttcc cgggtatcat cttcagtgaa
     7021 gcgcgcccgc tatggaatcc gaatatcgtg ctgtgcatga gtctttccaa gctggggctg
     7081 cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa tcatcactgc catcaccaat
     7141 atgaacggca ttatcagcct ggcacctggc ggtattggtc cggcgatgat gtgtgaaatg
     7201 attaagcgta acgatctgct gcgcctgtct gaaacagtca tcaaaccgtt ttactaccag
     7261 cgtgttcagg aaactatcgc catcattcgc cgctatttac cggaaaatcg ctgcctgatt
     7321 cataaaccgg aaggagccat tttcctctgg ctatggttta aggatttgcc cattacgacc
     7381 gagcagctct atcagcgcct gaaagcacgc ggcgtgctga tggtgccggg gcacaacttc
     7441 ttcccgggac tggataaacc gtggccgcat acgcatcaat gtatgcgcat gaactacgta
     7501 ccagagccgg agaaaattga ggcgggggtg aagattctgg cggaagagat agaaagagcc
     7561 tgggctgaaa gtcactaaaa attgcctgat gcgctacgct tatcaggcct acatgatttc
     7621 tgcaatatat tgaatttgga agaatttgta ggccggataa ggcgtttacg ccgcatctgg
     7681 cataaacaaa gcgcactatt ccggcgttga gaaacgccgg aaaacgtttc aaacaggctg
     7741 ctgccgtagc cgatgccgct gcactttctc gacatccatg aactgcaacg cctttgtcgg
     7801 gcaggcttca acacagggcg gccccgtctc ccgatgccag cacagatcgc acttaatcgc
     7861 cctcgccttt ttgcgcgaag aaacgacctc catcgcacca aacgggcaag ccagcataca
     7921 gcttttacag ccaatgcaac gtgtttgttc aacgaaaata tgcccatgtt cgcggcttat
     7981 cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca cactgatgac aggctactgc
     8041 cgtggtccag ctgtggtctt taatgacacg aatacgggaa ataaactcgt ctggtgacaa
     8101 cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa gccacttcac aggtacggca
     8161 accgatacat ttcgtcgcat ccgcaataat aaaccggttc atgatcactc ctttgcacta
     8221 aaacagcaga gtgccagaga gcgcgtccct actgctttga tccaacgaag gaaatgttaa
     8281 agttttggca cttgtccaaa gcgttatgtg atcgcgccca ggtcatccct gacagtaaat
     8341 cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg gtttcaggag atttttctct
     8401 cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca cggcgtacgg cacccgccca
     8461 tgaatatcaa acaccggaac agcaatacag gagacgccga gttcgttttc ttctctgtcc
     8521 atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga acatcgcggg cagctcggta
     8581 atggtattgc gggttaacgg ctggatctca tgctgatggt tttcccagta tgacttcacg
     8641 tagtccgggt gaccaaacgc catgtagatc ttgcccattg cggaacagta gagcggcata
     8701 tgctggccaa tataggcacg ggttcgcagc atcccggttg tgggttccag cttataaatc
     8761 aaaatagcgt gatcgtcttc gcggctggag aagttaatgg tttcaccagt ggcgatgttc
     8821 agtgcctcaa gatgcggagc ggcgatatgc atgatattca gcgaagacag cgccttctgc
     8881 ccgacggcaa taaatttggt agtcaggcgg taactccctg cggcaggcgc ggtggtcaca
     8941 tagccgcagg actgtaatcc ctgcaataag cgatggacgg tactcttatt taaaccagcc
     9001 agctccgaaa gatgcgccaa cggacaaccg tttggatagt tgctcaaaat ctcaatcagc
     9061 atcaacccgc gaaacagact ctggcttccg gctggacgct ctttttcctg cgccatctcg
     9121 ttctcttttt ttcccatcac ttctttcccc attttgtcgc gtcctgatgg tagcgcaaag
     9181 tgtgccgtag ttcacgatct cgacagataa tttataacca cttgattttt atgtcttttg
     9241 aaattcatca atcagatttc tttgttaaaa agtgatcgat atatttgaaa tcaagtttcg
     9301 cttattgaaa ttttaagcca aaaaagcgat caaaaaaaca aggaagcctg ggatgaaagt
     9361 gacatttgag cagttaaaag cagcctttaa tcgggtctta atttcacgcg gcgttgacag
     9421 cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc accgaatccg gcgtttattc
     9481 tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg gaaaacggcg atatcattcc
     9541 tgatgcccaa cccaaacgta taaccagcct cggcgcaatt gaacagtggg atgcccagcg
     9601 ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc gccattgaac tggctgccga
     9661 tcactgtatt ggtctggtgg cacttcgtaa tgccaaccac tggatgcgcg gcggcagcta
     9721 cggctggcag acggcggaaa aaggctatat tggcatttgc tggaccaact ccatcgccgt
     9781 aatgccgccg tggggcgcaa aagagtgtcg cattggcact aacccgctga tcgtcgccat
     9841 tccttccacg ccgatcacca tggtcgatat gtcgatgttc tcttacggca tgttagaagt
     9901 taaccgcctg gcgggccgtc agctcccggt cgatggtggc tttgatgatg agggcaattt
     9961 gaccaaagaa cctggcgtta tcgagaagaa tcgccgcatt ttgccgatgg gctactggaa
    10021 aggttctggc atgtcgattg tgctggatat gatcgctact ctcctttccg acggcgcatc
    10081 ggttgccgaa gtcacccagg acaacagcga cgaatacggc atttcacaaa tttttattgc
    10141 cattgaagtg gacaagctta tcgacggtcc cacccgcgat gccaagctgc aacgcatcat
    10201 ggattacgtt actaccgctg aacgcgctga cgaaaaccag gccatccgct tacccggcca
    10261 cgaatttact accctgctgg ccgaaaaccg ccgtaacggc atcactgttg atgacagcgt
    10321 gtgggccaaa atccaggcgt tatgaggaga taagtcatga tttttggaca tatcgcgcag
    10381 cccaatccgt gccgtttgcc cgccgccatt gaaaaggcgc tcgattttct gcgcgctacc
    10441 aacttcaacg tcctggagcc gggcgttgtc gaaatcgacg gcaagaatat ttatgcgcaa
    10501 attatcgatt taaccacacg cgaagcggtg gaaaaccgtc cgga
//
LOCUS       DAJTNA010000137        10352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTNA010000137 DAJTNA010000000
VERSION     DAJTNA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.138"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..351
                     /locus_tag="OO161_003017"
     CDS             <1..351
                     /locus_tag="OO161_003017"
                     /inference="COORDINATES: protein motif:HMM:NF026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464006.1"
                     /translation="ILNSLSRSPEESIPVSCRTWSETKAAYCCFSNDKVSANKIMAPH
                     KENITERAQQFKRVLVLQDTTELNYSGQKEKQGVGHKNIKMKETYFFILNSSSVSQVF
                     ASVFMMTISGSGMN"
     gene            complement(801..1994)
                     /locus_tag="OO161_003018"
     CDS             complement(801..1994)
                     /locus_tag="OO161_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5464007.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2130..3854
                     /gene="iucA"
                     /locus_tag="OO161_003019"
     CDS             2130..3854
                     /gene="iucA"
                     /locus_tag="OO161_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5464008.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3855..4802
                     /gene="iucB"
                     /locus_tag="OO161_003020"
     CDS             3855..4802
                     /gene="iucB"
                     /locus_tag="OO161_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5464009.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEHEIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4802..6544
                     /gene="iucC"
                     /locus_tag="OO161_003021"
     CDS             4802..6544
                     /gene="iucC"
                     /locus_tag="OO161_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5464010.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTNEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6541..7878
                     /gene="iucD"
                     /locus_tag="OO161_003022"
     CDS             6541..7878
                     /gene="iucD"
                     /locus_tag="OO161_003022"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5464011.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7884..10079
                     /gene="iutA"
                     /locus_tag="OO161_003023"
     CDS             7884..10079
                     /gene="iutA"
                     /locus_tag="OO161_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5464012.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2539 a         2639 c         2712 g         2462 t
ORIGIN      
        1 atcctcaata gcctcagtcg ttcaccagaa gaaagtattc ctgtttcctg ccgtacctgg
       61 agtgaaacaa aggcagctta ttgttgtttt tcaaacgata aagtctctgc taataaaatt
      121 atggctcctc acaaagaaaa tatcactgaa agagctcagc agtttaaaag agttttagtt
      181 ttacaggata caacagaact taattattct ggtcagaaag aaaaacaagg agttggtcac
      241 aaaaacataa agatgaaaga aacttatttc ttcatcctca actcgtcatc agtgagtcag
      301 gtgtttgcct cggtgtttat gatgactatc agtggttcag ggatgaatta aaaaccagta
      361 aaaatacccg acaagaaatc tgcaatgatt tacttcataa aaaacatgtc agtgaaaaag
      421 aaacctggcg ttgggttgaa ggatataata aagcaacaga actggctcgc caatgccctg
      481 atacccatgt attgtcaatt tctgatcgtg aaggcgattt ttatgacctg tttgaacgcg
      541 cagagcagac tcccggaata aaagctgact ggcttgtcag aatgaaattt aataacagag
      601 caacgctgaa tattaatggt aaacgggatc ataggttgtt gcacgaaaga atcatggaga
      661 ttacaccgca gcaactggtt gaattcacta tacctgatgg gcgggggcag caagccagaa
      721 cactgcctga catgctttca tcagacgcag gtcctctctc atatcctctg atttactcca
      781 gtaaggtgat gatcatatct ttatttgata gtacggtaat gccagataaa tacagacaca
      841 atggtaaata atatagttgc aaataatgct gccataatca gcgcgattgc gacacctgtt
      901 ttcgatacca gcggcaccag caaagcgcac agtccatagc caagtgtgtg actggtagcg
      961 agccagcccg cgcctgcgcc atcagccagc ttatcattca gtagaagttg ataagcgggt
     1021 gttgccagag ctgctccaaa tgacagcact gcacagcccg ggtaaaacaa ccatagctgt
     1081 tcggagagca tgatagccag accaccactc atcaacactc cggcactcag gagcagggcc
     1141 accggagtca ggcgctgcgg acggactacc ccgaactgcg cgataagcgc agctactgcg
     1201 gacagaccca acagccaggc cacctgttgg ctaatggcgg tggtatccgt gacgaactgg
     1261 cgagtaaggg caggcgaaag tccaagctgc atcatgctca ccgccgcagc cagtaatatt
     1321 gcgcaaagca gataaggcag acaatcccgc ttcagactga cgctcttgca ctccggtgtg
     1381 ggctgtggtg gtgtgccggg caaccgcagc agcatcagca gcgccagcac tggtgctgcc
     1441 atcagtagcc ccagtggcgc cagaggatga atggccagca ttgctgccgc gcacagcggc
     1501 ccgaatagcc tgccgcaact caggccggag ctgatggttg ccagagcggc catgcgattc
     1561 ccttctccag ctctctgcaa tgcccagacc tgacaggctg gcaccattgc cgacacggtc
     1621 agcccgtagg cgatccgggc gatgattaat attcccagtc ccaccgctgt tgttatggcg
     1681 ctggtagcca tcagcacgct tcccagccct agcagggtaa agcttatgcc atatcctccc
     1741 agagcccaca acaccacagg tttacttcca gtacgggaaa tttgctttcc ccaccacggg
     1801 gacgatggca gaaacagcat tgagccaagc atcagcaggg cagcccatac agacagactc
     1861 agatttgtct gtatgaccag aacagggagc acaaccagca gaccgttctg tccgataccg
     1921 agaagcccgg cactgaacgc aagtggccag caggacagtg ttttttgggg cgtatcttcg
     1981 atccctggtg acattataat gtttcaactc catgtattaa ttgtgtttat ttgtaaaatt
     2041 aatttatctg acaataacat ttattattga taatgagaat cattatcgac ataattgtta
     2101 ttattttact gtgtgggagc tgtttgacta tgaccctgcc ctctgaaaaa ccagccacag
     2161 atgtggctgc gcagtgcttc ctgaatgcac tgattcgtga aaccacagac tggaaactga
     2221 cagaataccc gccagacgaa ttgctcatcc cgctggatga gcagaaatcg ctccatttca
     2281 gagtggctta tttctcccca acccagcatc accgctttgc atttcctgca cgtctggtca
     2341 cggcatcagg cagttatcct gtcgacttta ccactctctc ccggcttatt attgataagc
     2401 tacgccatca actgtttctg cccgttcccc tctgcgaaac tttccaccag cgcgtgctgg
     2461 aaagccatgt ccatacgcaa caggcaattg atgcccgtca tgactgggcc gctctgcgtg
     2521 aaaaagcgtt gaattttggc gaggctgagc aggcgctgct gacaggacac gctttccacc
     2581 ctgcgcctaa gtctcatgaa ccgtttaacc ggcgggaggc tgaacgctac ctgcctgata
     2641 tggcacccca cttcccactg cggtggtttt cggtggataa aacgcaaatc gccggtgaaa
     2701 gtttgcatct caaccttcaa cagcggctga cgcgatttgc cgcagagaat gcgcctcagt
     2761 tactcaacga attaagtgac aaccaatggc tgttcccgtt gcacccgtgg cagggggaat
     2821 atcttttgca gcaggggtgg tgccaggcac ttgttgctaa agggctgatt aaagacttag
     2881 gtgaggccgg cacgtcgtgg ctgccgacca cctcttcccg ttccctctac tgtgccacca
     2941 gccgcgatat gatcaagttc tccctgagcg tacggctgac caactccatc cgtactctgt
     3001 ccgtgaaaga agtgaagcgt gggatgcgcc tggcacgcct ggctcaaacc gacggctggc
     3061 agatgctaca ggtccgcttc ccgactttcc gggtaatgca ggaggatggc tgggccgggc
     3121 tgctcgatct taacggcaac atcatgcagg aaagtctgtt tgccctgcgt gaaaatctgc
     3181 tggtggatca gccgaaaagc cagactaacg tactggtctc cctgactcag gccgcacctg
     3241 atggcggtga ttcgctgctg gtttcggcgg taaaacgcct gagcgatcgc ctcggtatca
     3301 ctgtgcaaca ggccgcccat gcatgggtcg atgcatactg tcagcaagtg ctaaagccgc
     3361 tgtttacggc tgaagcggat tacggcctgg tgctgctggc gcatcagcaa aatattcttg
     3421 tccagatgct tggggatctg ccggtcggat ttatttaccg tgactgtcag ggcagcgctt
     3481 ttatgcctca cgcgacagac tggctcgatt ctattggcga ggcgcaggcg gaaaatattt
     3541 tcacccatga gcagttgctg cgctatttcc cttattacct gctggttaac tccacttttg
     3601 ctgtgaccgc tgcgctgggg gctgccgggc tggacagcga atcgaatctg atggctcgtg
     3661 tacgagcatc gctggctgaa gtgcgtgatc aggtgactca taaaacctgt ctcaactacg
     3721 tgctggaaag tccgtactgg aacgtaaaag gtaacttttt ctgttatctg aacgatcata
     3781 acgagaacac cattgttgac ccttcggtga tctatttcga tttcgctaac ccgctgcagg
     3841 ctcaggaggt ctgaatgtct gaggcaaaca ttattcacag cagatatgga ctgcgctgtg
     3901 aaaaactcga caagcccctg aatcttggct ggggactgga caatagcgcg gtgttgcact
     3961 gtcccgggga gctgccgaca gggtggctgt gcgatgcgct ggatcagata tttatcgccg
     4021 caccacaact gtcagcagtg gctctcccct gggccgaatg gcgtgaggag ccacaggcgc
     4081 tgacgctttt tgggcaggta aaaagcgaca ttatccatcg tactgccttc tggcagttac
     4141 cgttatggtt gagttcaccg gcaaaccggg cctccggtga aatggttttt gatgcagagc
     4201 atgagattta tttcccgcag cgcccacccc gtccacaggg tgaggtttac cgccgttacg
     4261 atccacgtat tcgcaggatg ctgagtttcc gcattgccga tcccgtttct gatgcagaac
     4321 gtttcacccg ctggatgaat gatccgcgcg ttgagtattt ctgggagcaa agtggctcac
     4381 tggaggtaca gaccgcctat ctggagcgcc agctcaccgg taaacatgca ttcccgctga
     4441 ttgggtgctt cgacgatcgg ccattcagct atttcgaaat ctactgggcg gcggaagacc
     4501 gcattggtcg tcactattcg tggcagccct ttgaccgtgg cctgcatctg ctggttggtg
     4561 aacagcaatg gcgcggggcc cactatgtgc aaagctggct gcgcgggctg acacattacc
     4621 tgctgctgga tgagccccgt acgcagcgca cagtactgga gccacgcacc gataaccagc
     4681 gcctgttccg ccatcttgag cctgcgggat accggacaat taaagagttc gacttcccac
     4741 ataagcgctc gcgcatggtg atggcggatc gtcatcactt cttcacggag gtcggtctgt
     4801 aatgaatcac aaggactggg attttgttaa ccgtcggctg gtggcaaaaa tgttgtctga
     4861 gatggagtat gagcaggttt tccacgcgga atctcagggc gatgaccact actgcattaa
     4921 cctgccggga gcacaatggc gcttcatcgc tgaacgtggt atctggggct ggctgtggat
     4981 tgatgcccaa acgttgcgct gcacgaacga accagtactg gcgcagacgc tgctgatgca
     5041 gctaaagccg gtgctgtcaa tgagtgatgc cactgttgca gagcatatgc aggatttgta
     5101 tgccacgcta ctgggcgacc tgcaactgct gaaggcccgt cgcggactga gtgccagtga
     5161 cctgattaat cttgatgccg atcgtctgca atgcctgctg agcggtcatc ctaaattcgt
     5221 ttttaataaa ggtcgccgtg gctggggcaa agaggcgctg gaacgatatg cgccagagta
     5281 taccaacacc ttcagactgc actggctggc ggtaaaacgt gaacatatga tctggcgctg
     5341 cgataacgat ctggatattc agcagttgtt gactgccgca atggatccgc aggagtttac
     5401 acgcttcagt caggtctggc aggaaaacgg gctggatcac aactggctac cgctgccggt
     5461 acatccgtgg cagtggcagc aaaaaatcgc cacagacttc atcgctgatt ttgtcgaagg
     5521 caggatggtg tctctcggcg agtttggcga ccagtggctc gcccagcagt cgctgcgtac
     5581 cctgaccaac gccagccgcc ggggagggct ggatatcaag ctgccgctga ccatctacaa
     5641 cacctcatgc taccggggga tccctggcag atatatcgct gccggaccac tggcttcacg
     5701 ctggctgcaa caggtttttg cgaccgacgc caccctggtg caaagcggcg cagtgatcct
     5761 tggtgaaccg gcggcaggct atgtgtccca tgaaggctat gccgcgcttg cccgggctcc
     5821 ctatcgctac caggaaatgc ttggtgttat ctggcgggag aatccgtgcc gctggctgaa
     5881 accggatgaa agtcctgttc tgatggcaac actgatggag tgcgacgaaa acaatcagcc
     5941 gctggcaggc gcatatatcg accgctccgg actggacgct gaaacctggc ttacgcaatt
     6001 gttccgggtg gttgtggttc cgctgtatca cctgctttgc cgctacggtg tcgcgcttat
     6061 tgcacacgga caaaatataa cgctcgccat gaaagagggg gtaccacagc gtgttctgct
     6121 gaaagacttc cagggcgata tgcggctggt gaaagaagcg tttcccgaaa tggactcttt
     6181 gcctcaggag gtgcgtgatg ccacatcccg tctgagtgcg gactacttaa tccatgattt
     6241 gcagacgggt cacttcgtga cagtactgcg ttttatttcg ccactgatgg ttcgtcttgg
     6301 cgtacctgaa aggcgatttt atcaactgct ggcagcagtg ttgagtgatt acatgaacaa
     6361 acatccacaa atggcagagc gttttgcgct tttctcactc ttcaggccac aaatcattcg
     6421 cgtggtgctg aacccggtaa aactgacctg gccggacctg gatggcggca gtcgcatgct
     6481 gccgaattac cttgagaatc tgcaaaatcc gctgtggctg gtaactcagg aatatgaatc
     6541 atgaaaaaaa gtgtcgattt tattggtgtc gggacagggc catttaatct cagcattgct
     6601 gcattgtcac atcagatcga agaactgaac tgtctcttct ttgacgaaca tcctcatttt
     6661 tcctggcatc cgggtatgct ggtaccggat tgtcatatgc agaccgtctt tctgaaagat
     6721 ctggtcagtg cagttgcacc tacaaatccc tacagctttg ttaactatct ggtgaagcac
     6781 aaaaagttct atcgcttcct tacaagcagg ctacgtacag tatcccgtga agagttttct
     6841 gactatctcc gctgggctgc tgaagatatg aataacctgt atttcagtca taccgttgaa
     6901 aatattgatt tcgacaaaaa aagtcgattg tttctggttc agaccagtcg gggagaatat
     6961 tttgcccgca atatctgcct tggtacagga aaacaacctt atttaccacc ctgtgtgaag
     7021 catgtgacac aatcctgttt ccatgccagt gaaatgaatc ttcgtcggcc ggacctgagt
     7081 ggaaaacgga taaccgtggt tggtggagga cagagtggtg cagacctgtt ccttaatgca
     7141 ttacgcgggg aatggggaga agcggcggaa ataaactggg tctcacggcg taataatttt
     7201 aacgcactgg atgaggctgc ttttgctgat gagtatttta cacctgaata tatttcaggc
     7261 ttctccggac tgaaggaaga tattcgccat cagttactgg atgagcagaa aatgacatcg
     7321 gatggcatca ctgccgattc tttactgacc atttatcgtg agttgtacca ccgttttgaa
     7381 gttctgagaa aaccaagaaa tatccgtctg ctacccagtc gctcggtaac aactctggaa
     7441 agtagtggtc cgggctggag gttattgatg gaacatcatc tggatcaggg cagagagagc
     7501 ctggaaagtg atgtggtgat tttcgccaca ggttaccgtt ctgcattgcc acaaatactt
     7561 ccctcactga tgcccttgat caccatgcac gataagaaca cctttaaagt gcgtgatgac
     7621 ttcactctgg aatggagtgg cccgaaagaa aataacatct ttgcggtcaa cgccagcatg
     7681 caaactcatg gcatcgccga accccagctc agcctgatgg cctggcgatc tgcacgtatt
     7741 cttaatcgcg tactggggcg tgatttattc gatctcagta tgccgcccgc actgattcag
     7801 tggcgcagcg gcagccggaa aaaaccgcag ccggaggctg cttctttaac tcactataca
     7861 gcaaatattc aggaataacg atgatgcgca aaaagtatat gccccgggct cttggtccgc
     7921 tgcttcttgt cgtgctgtca ccagctgtcg cccagcaaaa cgatgataat gagatcatag
     7981 tgtctgccag ccgcagcaat cgaactgtag cggagatggc gcaaaccacc tgggttatcg
     8041 aaaatgccga actggagcag cagattcagg gcggtaaaga gctgaaagac gcactggctc
     8101 agttaatccc cggccttgat gtcagcagcc agagtcgaac caactacggt atgaacatgc
     8161 gtggccgccc gctggttgtc ctgattgacg gtgtgcgcct caactcttca cgttccgaca
     8221 gccgacaact ggactctgtc gatcctttta atatcgacca tattgaagtg atctccggcg
     8281 cgacggccct gtacggtggc gggagtaccg gagggttgat caacatcgtg accaaaaaag
     8341 gtcagccgga aaccatgatg gagtttgagg ctggcacaaa aagtggcttt aacagcagta
     8401 aagatcacga tgagcgcatt gccggtgctg tctccggcgg aaatgaccat atctccggac
     8461 gtctttccgt ggcatatcag aaatttggcg gctggtttga cggtaacggc gatgccaccc
     8521 tgcttgataa cacccagacc ggcctgcagc actccaatcg gctggacatc atgggaaccg
     8581 gtacgctgaa catcgatgaa tcccggcagc ttcaactgat aacgcagtac tataaaagtc
     8641 agggagacga caattacggg cttaatctcg ggaaaggctt ttccgccatc agcgggagca
     8701 gcacaccata cgtcagtaag gggctgaatt ctgaccgcat tcccggcact gagcggcatt
     8761 tgatcagcct gcagtactct gacagtgatt tcctgggaca ggaactggtc ggtcaggttt
     8821 actaccgcga tgagtcgttg cggtactacc cgttcccgac ggtaaatgcg aataaacagg
     8881 cgacggcttt ctcctcgtca cagcaggata ccgaccagta cggcatgaaa ctgactctga
     8941 acagccaact gatggacggc tggcaaatca cctgggggct ggatgctgag catgagcgct
     9001 ttacctccaa ccagatgttc ttcgatctgg ctcaggcaag tgcttccgga gggctaaaca
     9061 accataagat ttacaccacc gggcgctatc cgtcatatga catcaccaat ctggcggcct
     9121 tcctgcaatc cagctatgac attaatgata tttttaccgt tagcggtggc gtacgctatc
     9181 agtatactga gaacagggta gatgatttca tcgactacac gcagcaacag aagattgctg
     9241 ccgggaaggc gatatctgcc gacgccattc ctggtggttc ggtagattac gataactttc
     9301 tgttcaatgc tggtctgctg atgcacatca ccgaacgtca gcaggcatgg ttcaattttt
     9361 cccaaggggt ggcattgccg gatccgggga aatattatgg tcgcggcatc tatggtgcag
     9421 cagtgaacgg ccatctttcc ctgacaaaga gcgtgaacgt cagcgacagt aagctggaag
     9481 gcgtgaaagt cgattcttat gaactgggct ggcgctttac cggtgacaac ctgcggactc
     9541 aaatcgcggc atattactcg ctttccaata agagcgtgga aaggaataaa gatctgacca
     9601 tcagtgtgaa ggacgacagg cgccgtattt acggcgtgga aggtgcggtg gactacctga
     9661 tcccggatac tgactggagt accggtgtga acttcaatgt gctgaaaacc gagtcgaaag
     9721 tgaacggtca atggcaaaaa tatgacgtga aggaatcaag tccatcgaaa gcgacagctt
     9781 acattaactg ggcgccggaa ccgtggagtc tgcgtgtaca gagcaccact tctttcgacg
     9841 taagcgatgc agagggtaac gatattaatg gttacactac cgtcgatttt atcagtagtt
     9901 ggcagcttcc ggtgggaaca ctcagcttca gcgttgagaa cctcttcgac cgtgactata
     9961 ccactgtctg gggacagcgt gcacctctgt actacagccc gggttacggc cctgcttcac
    10021 tgtacgacta caaaggccgg ggccgaacct ttggtctgaa ctactcagtg ctgttctgac
    10081 ggggatgctt ttgcaacaag ggtatgctgt aaacaacatg gtccttggtg gccatgttgt
    10141 ttacagccag tataatatac agccttaaca agatattccg tctccggaat ggactgttca
    10201 ccactgaata acattatttc tgttttatgg atctttgact gaaatttatc gcatatcctc
    10261 atttcattgt ccgtctgaaa actaacctta caagtaagcg tcgtctcagc accgtctggc
    10321 agatcctgaa attcctgaga ggatgatctg cg
//
LOCUS       DAJTNA010000138        10306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTNA010000138 DAJTNA010000000
VERSION     DAJTNA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.139"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..77)
                     /gene="hokE"
                     /locus_tag="OO161_003024"
     CDS             complement(<1..77)
                     /gene="hokE"
                     /locus_tag="OO161_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5464013.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVG"
     gene            531..1649
                     /locus_tag="OO161_003025"
     CDS             531..1649
                     /locus_tag="OO161_003025"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5464014.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYYLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITGPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLEKKHCEIWAGD"
     gene            1715..1963
                     /locus_tag="OO161_003026"
     CDS             1715..1963
                     /locus_tag="OO161_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5464015.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2028..2396
                     /locus_tag="OO161_003027"
     CDS             2028..2396
                     /locus_tag="OO161_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5464016.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSEPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRALIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2490..3143
                     /gene="nfsB"
                     /locus_tag="OO161_003028"
     CDS             2490..3143
                     /gene="nfsB"
                     /locus_tag="OO161_003028"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5464017.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADSRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3251..4498
                     /gene="ybdG"
                     /locus_tag="OO161_003029"
     CDS             3251..4498
                     /gene="ybdG"
                     /locus_tag="OO161_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5464018.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(4566..5942)
                     /gene="pheP"
                     /locus_tag="OO161_003030"
     CDS             complement(4566..5942)
                     /gene="pheP"
                     /locus_tag="OO161_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5464019.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6044..9187)
                     /gene="cusA"
                     /locus_tag="OO161_003031"
     CDS             complement(6044..9187)
                     /gene="cusA"
                     /locus_tag="OO161_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5464020.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIVEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGGAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9199..>10306)
                     /gene="cusB"
                     /locus_tag="OO161_003032"
     CDS             complement(9199..>10306)
                     /gene="cusB"
                     /locus_tag="OO161_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR5464021.1"
                     /translation="RKILFWYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVR
                     IDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVG
                     DKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLI
                     ATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWL
                     VKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWL
                     QLNTASEPMLLIPSQGLIDTGSEQRVITIDADGRFVPKRVAVFQASQGVTALRSGLAE
                     GEKVVSSGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         2498 a         2862 c         2517 g         2429 t
ORIGIN      
        1 tccccgacca gaagcgtaaa tcccagcacc gttaaacaca gcactatgac tgccgcaagg
       61 gcatatttcg tcagcatgtt tatattgcct ccatgcgaag aagggactac catccaaatt
      121 gttcaggttt gggtaggtag ccccagggtt gatagaaata tcgtcctggg gcttcatctt
      181 tccagacctc agagtaaacc tgaaaccgga aagcttcagg cacccgcagt tatcttaccc
      241 ttgcttagca aaatagctgt ttatttatac agtaagtcct gtttcggact tctcactccc
      301 catttgatca actgaagctg aacccgtttt tttctgcatg cctcaatcgt caaaattctt
      361 cgccaccagt cagcactgcc ataacttgtc acattgcaaa agtagccaaa ttgcttctgt
      421 cataatcagt tcaccatcac caaaaaaaag aaacattcca gaacttccgc atctcgccca
      481 gccgctccca cttctatact gaatagaaaa cgccaacata agagaaacct atgccattac
      541 ccgattttca tgtttctgaa ccttttacac tcggtattga actggaaatg caggtggtta
      601 atccgccggg ctattactta agccaggact cttcaatact gattgacgcg gttaaaaata
      661 agatcacggc cggagaggta aagcacgata tcaccgaaag tatgctggag ctggcgacgg
      721 atgtttgccg tgatatcaac caggctgccg ggcaattttc agcgatgcag aaagtcgtat
      781 tgcaggcagc cgcagaccat catctggaaa tttgcggcgg tggcacacac ccgtttcaga
      841 aatggcagcg acaggaggta tgcgataacg agcgctatca acgaacgctg gaaaactttg
      901 gttatctcat tcaacaggcg accgtttttg gtcagcatgt tcatgttggc tgcgccagtg
      961 gcgatgacgc catttatttg ctgcacggcc tgtcacggtt tgtgccgcac ttcatcgccc
     1021 tttccgccgc gtcgccatat atgcaaggaa cggatacgcg ttttgcctcc tcacgaccga
     1081 atattttttc cgcctttcct gataatggcc cgatgccgtg ggtcagtaac tggcaacaat
     1141 ttgaagccct gtttcgctgt ctgagttaca ccacgatgat cgacagcatt aaagatctgc
     1201 actgggatat tcgccccagt cctcattttg gcacggtgga ggttcgggtg atggataccc
     1261 cgttaaccct tagccacgcg gtaaatatgg cgggattaat tcaggctacc gcccactggt
     1321 tactgacaga acgcccgttc aaacatcagg agaaagatta cctgctgtat aaattcaacc
     1381 gtttccaggc ctgtcgctat gggctggaag gcgtcattac cggtccgcac actggcgatc
     1441 gtcgaccgct aacggaagac accttgcgat tgctggaaaa aatcgcccct tccgcacata
     1501 aaattggtgc atcgagcgcg attgaggccc tgcatcgcca ggtcgtcagc ggtctgaatg
     1561 aagcgcagct gatgcgcgat ttcgtcgccg atggtggctc gctgattggg ctggagaaaa
     1621 agcattgtga gatctgggcc ggtgactaag tagggaattg ccttacagaa gaatatccct
     1681 gacaatggtg ttttaactta catttaacaa tgttatgaaa caccctttag aaaccttgac
     1741 taccgcagca ggcattttgc tgatggcttt cctctcttgc ctgctgctgc ccgcccccgc
     1801 actggggctg acgctggcac aaaaactggt gaccatgttc catctgatgg atcttagcca
     1861 gctttacact ttattgtttt gtctgtggtt tttagtgctg ggcgctattg agtattttgt
     1921 tctgcgcttt atctggcgac gctggttctc gctggcggat taaacgtcga ttatcgccgt
     1981 acttcgcggc atactttgct tattctcctt cgcctaaagg aacgtttatg gataagcaat
     2041 cactgcacga aacggcgaaa cgcctggccc ttgagttacc ctttgtcgag ctttgctggc
     2101 cttttggccc ggagttcgat gtttttaaaa ttggcggcaa aatttttatg ctgtcgtcgg
     2161 agctgcgcgg cgtccccttt atcaatctga agtccgaacc acaaaaatcc ctgttaaatc
     2221 agcagatata cccgagcatt aagccagggt atcacatgaa taaaaagcac tggatttcgg
     2281 tgtatcccgg cgaggaaatc tccgaagcgt tacttcgcgc tctgatcaac gattcgtgga
     2341 atctggtggt tgatggtctg gctaaacgcg atcaaaaaag agtgcgtcca ggctaaagcg
     2401 gaaatctata gcgcattttt ctcgcttacc atttctcgtt gaaccttgta atctgctggc
     2461 acgcaaaatt actttcacat ggagtcttta tggatatcat ttctgtcgcc ttaaagcgtc
     2521 attccactaa ggcatttgat gccagtaaaa aacttacccc ggaacaggcc gagcagatca
     2581 aaactctcct gcaatacagc ccatccagca ccaactccca gccgtggcat tttattgttg
     2641 ccagcacgga agaaggtaaa gcgcgtgttg ccaaatccgc tgccggtaat tatgtgttca
     2701 acgaacgtaa aatacttgat gcctcgcacg tcgtggtgtt ctgtgcaaaa accgcgatgg
     2761 acgatgcctg gctgaagctg gttgttgacc aggaagatgc tgatagccgc tttgccacgc
     2821 cggaagcgaa agccgcgaac gataaaggtc gcaagttctt cgctgatatg caccgtaaag
     2881 atctgcatga tgatgcagag tggatggcaa aacaggttta tctcaacgtc ggtaacttcc
     2941 tgctcggcgt ggcggctctg ggtctggacg cagtacccat cgaaggtttt gacgccgcca
     3001 tcctcgatgc agaatttggt ctgaaagaga aaggctacac cagtctggtg gttgttccgg
     3061 taggtcatca cagcgttgaa gattttaacg ctacgctgcc gaaatctcgt ctgccgcaaa
     3121 acatcacctt aaccgaagtg taattctctc ttgccgggca tctgcccggc tatttcccct
     3181 cagattctcc tgatttgcat aaccctgttt cagccgtcat cataggctgc tgttgtataa
     3241 aggagacgtt atgcaggatt taatatccca ggttggggat ttagcgggta ttgagatcga
     3301 tcacaccacc tcgatggtga tgattttcgg tattattttt ctgaccgccg tcgtggtgca
     3361 tattattttg cattgggtgg tactgcggac cttcgaaaaa cgtgccatcg ccagttcacg
     3421 gctttggttg caaatcatta cccagaataa actcttccac cgtttagctt ttaccctgca
     3481 ggggattatc gtcaatattc aggcggtatt ctggctgcaa aaaggcaccg aagcggcaga
     3541 tattctgact acctgcgcgc agttgtggat catgatgtat gcgctgcttt cagtcttctc
     3601 gttgctggat gttattttga atctggcgca gaaattcccg gcggcatctc agttaccgct
     3661 gaaagggata tttcagggga ttaaactgat cggcgcaatc ctggtcggca ttttgatgat
     3721 ctcgctgctg attggtcagt cgccagcgat tctgatcagc ggtcttggtg caatggctgc
     3781 cgtgctgatg ttggtattta aagatccgat tcttggtctg gtggcaggta ttcagctttc
     3841 cgcgaacgat atgctgaaac tgggcgactg gctggagatg ccgaaatacg gcgcggatgg
     3901 cgcggtgatc gatattgggt taaccaccgt caaagtgcgt aactgggaca ataccattac
     3961 cactattccc acctggtctc tggtttctga ctcctttaaa aactggagcg ggatgtcagc
     4021 atctggcggg cgacgtatta agcgcagtat cagtattgat gtcaccagta ttcgttttct
     4081 tgatgaagac gaaatgcaac gtctgaataa agcgcacttg ttaaaacctt atttaaccag
     4141 ccgccatcag gaaattaatg agtggaatcg ccagcaaggt tctacggagt cgatattaaa
     4201 cctgcgccga atgaccaata ttggaacctt tcgtgcctat ctgaacgaat atctgcgtaa
     4261 ccatccgcgg attcgtaaag atatgacctt aatggtacgc caactggctc caggtgataa
     4321 cggtttaccg ctcgagatct acgcgtttac caacaccgtg gtgtggctgg aatatgaaag
     4381 cattcaggct gatatattcg atcacatatt tgcgattgtc gaagagtttg gtctgcgact
     4441 tcatcagtcg ccagccggca atgatattcg ctctctggcg ggtgcattta agcagtaatt
     4501 aaaaaaaccg ctctcatcga atggatgaga gcggacatcg ttttgtcgga tgtagcgtga
     4561 atgccttatt tccgacgcag cgttttaaat gccacaaaca ggaatacaat ccacaccggc
     4621 agcaggatcg ctgacaagcg catatcatcc atcgtgcaca tcagcagcaa aatcatgccg
     4681 aggaaggcaa tgcaaagata gttgccgaac ggataaagca gcgccttaaa ctgtgtttca
     4741 cgtccctgac gtcgcatcgc cgcgcgaaaa cgcagatgcg ccaggcagat cataatccag
     4801 ttcaacagca gcgttgctac caccagcgcc atcagcagac caaacgcttt ttgcggcagc
     4861 agatagttga ttaacaccac cagcgaagtg atcgctccgg aaagcatcag cgagttaatc
     4921 ggcacgccgc gatggctgac gcgagtcaaa aacttcggcg cattaccctg cacagaaagg
     4981 ccaaacagca tgcggctgtt agagtaaacc ccgctgttat acactgacag cgatgctacc
     5041 agaatgacga agttcagcgc agaagctacc tcgttgctgt cgagattatg gaaaatcatc
     5101 acaaacgggc tactgttgga tttcacttcc acccacggat agagcgccag taaaaccacc
     5161 agtgaaccga tgtaaaacag caggatgcga tacaccacct gatttaccgc ttttggaatg
     5221 cttttttccg gatcgcgcgc ttcagcggca gtaatcccaa tcagctccag accgccgaag
     5281 gagaacataa ttaccgccag cgacaaaatc agcccattcc agccggtggc gaagaaacca
     5341 ccgtagcgcc agaggttgtc gatactggct ttctcgccgc cgtgaccaga aaacagcagc
     5401 cacaggccaa agccgatcat accgatgatt gccagcactt taatcaacgc aaaccagaac
     5461 tcggtttcgc catataagcg cacgttcacc aggttaacgg cgttgatgat aataaagaag
     5521 gcggcagccc aaatccacgt tggaacatcc gggaaccagt actgcatata gatgcccgca
     5581 gcggtcagct ctgccattcc caccagcacg aacattaccc agtagttcca gccagagagg
     5641 aagcccgcaa acggtcccca gtatttatag gcaaagtggg caaatgaacc ggataccggc
     5701 tcttcgacca ccatctcgcc gagctggcgc ataatcagga aagcgatgat cccggcgacg
     5761 ccgtagccca gcaatacagc cggacccgcc atctgaatcg ccgggccaat gccaagaaac
     5821 agaccagtac caattgcgcc acccagcgca atcagttgaa tatgacggtt atgtaatccg
     5881 cgatgaagcg tcggctcttg attcgacgca gtatcttccg atacggttga cgcgtttttc
     5941 acgcctttcc cctgtgtgtc ttttttgttg aggggcacct tttaacattt agtgcccatc
     6001 gtcgcaagac acaatccaca cggttaaacg gggtatcctg cttttatttc cgtacccgat
     6061 gtcggtgcag ccacatcagc ttatacgccg ccgggataat aaacagcgac agcaaaggcg
     6121 cggtgatcat gccgccaatc atcggtgcgg caatccggct catcacctct gaaccagccc
     6181 ccgttcccca cagaatcggc agcagaccgg cgataatcac cgccaccgtc atcgctttcg
     6241 ggcgcacgcg cagtaccgcg ccgtgataca acgcctcatc cagcttctgc tcactgaatg
     6301 tttgcggatt attcaacgac ggctcggcct ctatggcgtg acgtaaatac atcagcatca
     6361 ccacgccaaa ttcggcggcg cccccggcaa gggcaataaa accggtgccc gttgccacag
     6421 aaagatgaaa gcccatccac cacagcaacc agatgccgcc caccagcgca aacggtacgc
     6481 tgctgataat cagcaacgct tcgcccaccc gacggaatgc cagatacaac agcacgaaga
     6541 tgatcatcag tgtcatcggc accatgagtt tcagcttatg gttggcgcgc tccagtagct
     6601 cgaactgccc ggagaatgcc acgctggtgc ccggttttaa ctgcactttt tcaactatcg
     6661 ctttttgcaa atcgtgaacc accgacacca tgtcacgatc gcgggcatcg atataaatcc
     6721 agctcgtcgg gcgcgcattc tcggttttca gcatcgacgg tccggttgag actttaacgt
     6781 cggccacgtc tgccagggtg atttgctgct tcatcggcgt caggatcggt agctggcgca
     6841 gtgcctgcgg gctatcgcgc cagctttgcg gataacgcag attaatggga taacgggcaa
     6901 tcccttccac cgtttcgcca accatcgccc cgcccaccgc agaagtcaca aacaactgca
     6961 catccgccac cgtcataccg taacgcgcgg ctttttcacg gttaatctca acgttgatat
     7021 agcgcccacc ttccagacgc tcggcaagcg cagaagccac gcctggcacc gttcgcgcta
     7081 cttcttcaat ttgctcagcc atcgcgtcga tatccgccag cacagtgccg gaaactttaa
     7141 tgccgatggg gcttttaatg ccggttgaga gcatatcgat acggttacga attggcggca
     7201 cccacagatt cgccagcccc ggcagccgca cggtgttatc cagttcctca atgattttgt
     7261 ccatcgtcat gccaggccgc cactgatcct gcggcttaag ctggatggtc gtttcaacca
     7321 tctcgagcgg agcagaatcg gtggcggttt ccgctttccc ggttttgcca aatacccgcg
     7381 ccacttcagg tacgctcata attagcttgt cggttttttg cagcatactc gccgcctctg
     7441 ctgcggaaat ccccggcagc gtcgatggca tatacaacaa gtcgccttca ttgatctgcg
     7501 gtaaaaattc cccgccaact ttattgagcg gccagagaac cgtcagcacc gaaagcgctg
     7561 ccaccaacag cgtggttttc ggccagtgca gtactttcag caatagagga tgataaacac
     7621 gaatcaaaaa gcgattgagc gggttactgc tttccggcgg aattttgcca cggatccagt
     7681 aacccatcag gatcgggatc actacgatcg ccagcagcgc cgcacccgcc atcgcatacg
     7741 ttttggtgaa cgccagcggg ccaaacagac gcccttcttg cccttccagg gtgaagattg
     7801 ggataaacga cagcgtgata atcagcagac tgataaacag cgccggcccc acttcaacag
     7861 atgcatcggt aatcacctgc cagcgcgttt tattatccag cgtggcgtca ggatgctggt
     7921 gctgccactc ttccagccgt ttatgcgcat tctcgatcat gacgatagca gcatcgacca
     7981 tcgccccgac ggcaatcgca atgccaccca acgacataat attggcattc agtccctgga
     8041 agtgcatgac aataaaagca atacacaacc ccagcggcaa cgaaataatc gccaccagcg
     8101 ccgagcgcac atgccagaga aacagcgcac agactaccgc caccacaata aactcttcca
     8161 gcaacttgcc gctgaggttg tcgatggcgc ggtcaatgag ctggctgcga tcgtatgttg
     8221 taactatctc cacgccttcc ggcagactac ttttcagcgt ttccagtttg tccttcacgg
     8281 cggcgatcac ttctcgggcg tttttgccgg agcgcaggat caccaccccg cccgccactt
     8341 cgccttcgcc gttcagttcg gcaatgcccc ggcgcatctc cgggccaacc tggaccttcg
     8401 caacatcgcg cagataaacg ggcacgccat tttcactggc ttttaaaacg atgtgattaa
     8461 agtcgtcgag cgtttgcaga tagccgctgg cgcgcaccat atattccgct tccgccagtt
     8521 cgatcgacga accgcccgct tcctggtttg aagcatccag cgcgcttttt acttcggcga
     8581 gactgatgcc atactgcgcc aggcgctggg gatcgataac cacctgatac tctttcacca
     8641 caccgcccac cgacgccact tccgcaacgt cagggatggt tttcagctca tatttgagaa
     8701 accagtcctg taatgagcgt aaatcggcca gatcgtgctt accgctgcga tccaccagtg
     8761 catattcata gatccagcca acacccgtgg catctggccc cagctcggcg ctgactcccg
     8821 caggcagctt accctgtacc tggttgaggt actccagcac ccgcgaacgc gcccagtacg
     8881 gatcggtacc atcttcgaaa atgacataca cataagagtc gccaaactgc gagaaaccac
     8941 gcacagtctt cgcgccaggc accgacaaca tggtggtggt tagcggataa gtcacctgat
     9001 tttcaacgat ttgcggtgcc tgcccgggat agctggtttt aataatcacc tgcacatcgg
     9061 agagatccgg cagcgcatcc actggcgtat taatgatggt ccaggtgccc cagatgctaa
     9121 gaaacaacgc gcccatcagc accaaaaaac ggttcgccac cgagcgacga ataatccatt
     9181 caatcattgg ttattccctc aatgcgcatg ggtagcactt tcagagcgca tccgctccag
     9241 tgcgccagaa atattggctt cggaatcaat caggaaaagg ccgctggaaa ccaccttttc
     9301 accttccgcc agaccagagc gtaatgcggt gacaccttgc gatgcctgga aaacggcaac
     9361 gcgtttcggt acaaagcgcc cgtcggcatc aatggtaatc acccgctgtt cgctaccggt
     9421 atcaatcagc ccttgtgacg gaatgagcag catcggttcg ctggcggtat tgagttgcaa
     9481 ccaggcgttc atgcccggtt ttagcgcctc gtcggcgttg tcgacttcca gacgcagctg
     9541 cagcgtgcgg gtcgcggcat ccacgccggg tagcagcgtc catttgcgga tggtgagtgt
     9601 tttatccggt cgtgccggaa cggtgagcgt aaactgcgag gcatctttca ccagccaggc
     9661 gatagactcc gggatcgcag cagtgaccca caccgggtcc ataccctgaa ttttcgctac
     9721 cacgttatct ttggcgatat tcattcccgc gcgcagatca aacgcggtga tcacgccatc
     9781 aatgggcgct ttgagcgtaa agcgagtctg gattttttgc gtggcgatca ggcggcgaat
     9841 atccgcctcc ggcattcccg ccagtcgcag ccgctcaaga atgccttcag tctgggtcgc
     9901 cgtaccgccg gtttcgcgca gcagtaaata ctcactctgc gcttccaccc agtcaggaat
     9961 ggtcaggtcg agaagcggtg tgcccttttg tactttatcg cccacggtaa gcggatacac
    10021 cttgtcgata aacccggcag cgcgagcctg cacaatggca tactgatact cgttgtaact
    10081 gacattcgcc gggaaactct gggcaaaagt cagcggtccg cgcgtgacgg tagccgtttt
    10141 cacccccaga ttctgcgtct gagtcgggtc aatgcgcaca ccagacgcag aactctcttc
    10201 atcggcatat ttcggcacca gatccatatc cataaacggc gatttacctg gtttatcgaa
    10261 ccgcgtattg gggtacatcg ggtcgtacca gaataagatt ttacgt
//
LOCUS       DAJTNA010000139        10075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTNA010000139 DAJTNA010000000
VERSION     DAJTNA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.140"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            318..1364
                     /locus_tag="OO161_003033"
                     /pseudo
     CDS             318..1364
                     /locus_tag="OO161_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
     gene            complement(1361..2152)
                     /gene="nei"
                     /locus_tag="OO161_003034"
     CDS             complement(1361..2152)
                     /gene="nei"
                     /locus_tag="OO161_003034"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5464022.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2188..2922)
                     /gene="pxpA"
                     /locus_tag="OO161_003035"
     CDS             complement(2188..2922)
                     /gene="pxpA"
                     /locus_tag="OO161_003035"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5464023.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEQALAFARRLRSTFAEKEIVVAA"
     gene            complement(2912..3844)
                     /gene="pxpC"
                     /locus_tag="OO161_003036"
     CDS             complement(2912..3844)
                     /gene="pxpC"
                     /locus_tag="OO161_003036"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR5464024.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3838..4494)
                     /gene="pxpB"
                     /locus_tag="OO161_003037"
     CDS             complement(3838..4494)
                     /gene="pxpB"
                     /locus_tag="OO161_003037"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5464025.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGNLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4517..5260)
                     /gene="ybgI"
                     /locus_tag="OO161_003038"
     CDS             complement(4517..5260)
                     /gene="ybgI"
                     /locus_tag="OO161_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5464026.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5531..6922
                     /gene="dtpD"
                     /locus_tag="OO161_003039"
     CDS             5531..6922
                     /gene="dtpD"
                     /locus_tag="OO161_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5464027.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAVGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQIT"
     gene            complement(7054..8472)
                     /gene="phrB"
                     /locus_tag="OO161_003040"
     CDS             complement(7054..8472)
                     /gene="phrB"
                     /locus_tag="OO161_003040"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5464028.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8469..8978)
                     /locus_tag="OO161_003041"
     CDS             complement(8469..8978)
                     /locus_tag="OO161_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5464029.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9262..9636)
                     /locus_tag="OO161_003042"
     CDS             complement(9262..9636)
                     /locus_tag="OO161_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464030.1"
                     /translation="MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKY
                     ENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLILMADFGESRYFKPAKI
                     AMNKNNLIIRTSDVEIAFKITK"
     gene            complement(9621..>10075)
                     /locus_tag="OO161_003043"
     CDS             complement(9621..>10075)
                     /locus_tag="OO161_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5464031.1"
                     /translation="LGLIDKPAPVFNRDLNSDPYYLAVNNCYSYALNRYGNPGSRIFG
                     GGGLQPGELSGKEFSKLTCGSIFEASKNDGAKDLDNGSCPSGYHKAQLFIRPHNFIGM
                     GGDYHWYRQDANGEWSDKQGVGAIRFRGKDPFPPIDYPEKCGTIFLPN"
BASE COUNT         2339 a         2744 c         2420 g         2572 t
ORIGIN      
        1 cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt
       61 ggtctacgaa aaaacctata cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc
      121 tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc
      181 ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcctct tttccttgat
      241 tactgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa
      301 taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc
      361 tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga
      421 tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc
      481 tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca
      541 ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca
      601 gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct
      661 agggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag
      721 atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg
      781 ttctggtcac ctgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct
      841 ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta
      901 cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg
      961 cagtgctcca gtctggtcag ctcatcacca tcgaactacc ggaatggctg ctggcgatgg
     1021 cgtatatggc aattggctgg cggattggtc ttggtttcgg taaacaaatc ttactgcggg
     1081 cactacgccc actaccgcaa atcctgctgt cgatttttgc tctgctggct atttgtgcag
     1141 gtatggcgtg gggactgacc cggtttatgc atattgattt tatgactgcc tacctcgcca
     1201 ccagtcccga cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg
     1261 cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggccagcca
     1321 ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc ctagtgctgg cagcctggac
     1381 accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca cagcgttcgc
     1441 acagttcgcc atcacgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct
     1501 catccacctg cccccgcgta gcgtaagaaa accgaggaat ctccagtaac gcgtgtgcga
     1561 gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct
     1621 gccagaggat ctccacccgc aaataattgc caagcccagc cagaaacgcc tgatcgagca
     1681 gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc tttaccacct
     1741 ccggcgtcag attcggatcc agcacatcgg ggccaacgcg ttgtaaaaac ggatgcgtgg
     1801 tcagttgttc cggcctcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt
     1861 cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa
     1921 ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaagtcg ttggaaaaat
     1981 gagttaacaa cgccttacca cgcgttccca catgggtaac gtgttgaccg ataagttgtg
     2041 attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga
     2101 tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat
     2161 ccttttttca ttctttatta atcggggtta tgctgcgaca acaatctcct tttcggcaaa
     2221 tgtagagcgt agtcggcggg cgaacgccag tgcttgctcg ccgtcgccat gcaggcagac
     2281 tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac
     2341 catctctagc gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc ctggctgact
     2401 tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt
     2461 tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaac
     2521 caatgctgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt
     2581 cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc
     2641 acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg
     2701 cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc
     2761 aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gggcatcgcc
     2821 tgcatgaaat ccacaagcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct
     2881 ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgtagc cgccacgcta
     2941 attgttcgaa ataacgttgt tgatcttgcc gcgctttcag tgcctcttcc agtgaacact
     3001 ggacaaaatg aatcggctga ccgagcggaa tttgcgccag atggtacata tcagcctcaa
     3061 tgatacaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg
     3121 gctgcccgtt atgcggcacc tgcaccacac ccggtaacaa accgtgagat aacagttcgc
     3181 gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg
     3241 ggctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat
     3301 attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg
     3361 cttccataaa atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc ttcagtaaac
     3421 ggccttccag cccgccaatc cccacgttga gatcggtgct gcatgacccc attaccggcg
     3481 gaacatcaat accacccgcg accgccagat aactgcgcat gccgtgctgc gggcgtttaa
     3541 gcgttaaacg ctggcctgct ttcatcggca agcgccagcc ggtccagacg gcgttatcat
     3601 ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa
     3661 ccgttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagca
     3721 ggttagcaat gcgtaacgca ggcatatcca gtgcgccgca gtggctgata cccgactggc
     3781 gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca
     3841 gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg
     3901 ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc
     3961 cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg
     4021 aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aattcccgag
     4081 atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa
     4141 ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc
     4201 gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc
     4261 gctctcctcc caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt
     4321 acgcaaaatc accgtgatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc
     4381 caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc
     4441 cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga
     4501 tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg
     4561 tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac
     4621 ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt
     4681 cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc
     4741 cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg
     4801 gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg
     4861 tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg
     4921 ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat
     4981 acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga
     5041 ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac
     5101 gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg
     5161 tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca
     5221 gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct
     5281 gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac
     5341 atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc
     5401 taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt
     5461 tttaacttta aggaaacaca agcatgccgc gcaactatta cgtgacgttg ccgtcaattg
     5521 tggaattatt atgaataaac acgcatcaca gccgcgcgct atttactatg tcgttgcgct
     5581 gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac
     5641 caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct
     5701 ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat
     5761 ggcggtgatg ctgggggcgt tattgatggc gatcggtcat gtggtgttgg gtgccagtga
     5821 gatccatccg tcattcctct atctgtccct ggcgataatc gtttgtggct atggtctgtt
     5881 taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga
     5941 tggcggtttc tcgctgatgt atgcagtggg taacgtgggg tctattatcg cacctattgc
     6001 ctgtggctac gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg
     6061 tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg
     6121 cgttaataaa aaagtactgc gtgcgacaaa ctttctcctg cctaactggg gatggctgct
     6181 ggttctgctg gtggcaacgc ctgcgctgat taccgtattg ttctggaaag agtggtcggt
     6241 atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa
     6301 agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag
     6361 tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg
     6421 cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa
     6481 tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc
     6541 aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggtctcggcc tgatgagcgc
     6601 cgggttctgc attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct
     6661 gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt
     6721 tgccatgtcg caaattacgc gcattgaaat ccccggtgtg accggcgtat taaccggcat
     6781 ttatatgctg ctttccggcg cgattgcgaa ctatctcgcg ggcgtgattg ccgatcagac
     6841 atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga
     6901 agtatttgat caaattacct agggcgcact ggcgtgtgta ggactggtac tgatgatttg
     6961 gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat aatagaccag
     7021 caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta
     7081 cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag
     7141 catcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc
     7201 gcgcagctcc ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt tctcgccctg
     7261 ggttgtcggg ttgaaaatac gaaaatacgg cgctgcatcg gttccggttg aagcggccca
     7321 ctgccagcca ccgttattgg ctgccaaatc accatcaatc agctgcgaca tgaaatatcg
     7381 ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg
     7441 tagccggtta tgcatccagc cagtgctgtt aagctgacgc atggcagcat cgacaatcgg
     7501 gtatcccgtt ttgccttcct gccaggcctg taaatgtgcg ggattgctct gccactgcac
     7561 acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag
     7621 atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc
     7681 cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc
     7741 cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg
     7801 ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc
     7861 ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg
     7921 cgagggctct atcgatccgc tactacgaac ttttggcgca gcgacgcact ccggcatccc
     7981 ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg
     8041 attaccggtc atcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac
     8101 cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg
     8161 atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac
     8221 actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc
     8281 tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg
     8341 cgtcgcccac tggcgtggtg tagcgatata caacgccagc acgcgtgcag acgaattgcg
     8401 gcaggcggca gccagtgcga gattatcgtg caaacgtaaa tcctggcgaa accagaccag
     8461 atgggtagtc ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa
     8521 taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc
     8581 agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc agccagctgc
     8641 tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacattg
     8701 gtgtgatcgc gcgggttggc ggggtgagaa agcaaaacaa ctacgcgttg gcggtactgg
     8761 ttgtaatagt catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg
     8821 cggtactccg gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga
     8881 agcgccccgc gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcaaagata
     8941 cggattaggg tgctgtcatc aaatcgttgt agattcatta ctgctcctga acggtataaa
     9001 ggagtaagtg tagacgatgg taaatgaatt ttatggggca gagtttttgc ggggaagtat
     9061 tttgttggta aaaatattat caacttcatc tgagcgagaa ttattctact atttcccgag
     9121 ctgcattcat tttttgtatt ttttattttt agaaaataac agttctcaaa gcttaattac
     9181 ctcactatta attgtatttc aaactaacaa tcgcactcca ttttctcttt ttaaaaaaat
     9241 aaacactgtt aaaataaata tttatttcgt tatcttaaat gctatttcta catcactcgt
     9301 ccttataatc aaattattct tattcatagc tatttttgca ggtttaaaat agcggctttc
     9361 accaaagtca gccatgagaa ttagactaca agttctccca gcctttgcac atgagaaaaa
     9421 ccacccatca actctattta ttcctgaacc tttaacacca taataaatca gaatttctgg
     9481 gtattgttca tccttcacgt tttcatattt ttggtattta attgaaaacc aagagtctat
     9541 actgccaaca tcttcaaaaa atattggagc tgatgtttcc gcatgaacat atgcagaaaa
     9601 tgctaacaat atggcaatac ttagtttggt aaaaatatcg ttccacactt ttcaggataa
     9661 tctattgggg ggaaggggtc ttttcctcta aatctaattg ctcccactcc ttgtttgtca
     9721 gaccattcgc catttgcatc ctgacgatac cagtgataat cccctcccat gcctataaaa
     9781 ttgtgaggac gaataaacaa ttgagcttta tggtaaccag atggacaact cccattatct
     9841 aaatccttgg caccatcatt ttttgacgct tcaaaaatac taccgcaagt gagtttagaa
     9901 aactcttttc cagataattc tccaggttgt aatcctccgc cgccaaaaat cctactccca
     9961 gggtttccat acctatttag agcatatgaa taacaattat ttacggcaag ataatacgga
    10021 tctgaattta ggtctcgatt gaatactgga gcaggtttat caattaagcc cagcg
//
LOCUS       DAJTNA010000140        10063 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTNA010000140 DAJTNA010000000
VERSION     DAJTNA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.141"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..212
                     /locus_tag="OO161_003044"
     CDS             <1..212
                     /locus_tag="OO161_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464032.1"
                     /translation="HASTLKSPSYTKSVSWQHYRHICCYRRKLTDAELEACYRYDKQF
                     WEHYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(329..1672)
                     /gene="gdhA"
                     /locus_tag="OO161_003045"
     CDS             complement(329..1672)
                     /gene="gdhA"
                     /locus_tag="OO161_003045"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5464033.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1908..2180
                     /locus_tag="OO161_003046"
     CDS             1908..2180
                     /locus_tag="OO161_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5464034.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2146..2553)
                     /gene="nudG"
                     /locus_tag="OO161_003047"
     CDS             complement(2146..2553)
                     /gene="nudG"
                     /locus_tag="OO161_003047"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5464035.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            2640..3250
                     /locus_tag="OO161_003048"
                     /pseudo
     CDS             2640..3250
                     /locus_tag="OO161_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
     gene            complement(3259..4566)
                     /gene="ynjE"
                     /locus_tag="OO161_003049"
                     /pseudo
     CDS             complement(3259..4566)
                     /gene="ynjE"
                     /locus_tag="OO161_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(4633..5286)
                     /locus_tag="OO161_003050"
     CDS             complement(4633..5286)
                     /locus_tag="OO161_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5464036.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5286..6822)
                     /locus_tag="OO161_003051"
                     /pseudo
     CDS             complement(5286..6822)
                     /locus_tag="OO161_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(6795..7961)
                     /locus_tag="OO161_003052"
                     /pseudo
     CDS             complement(6795..7961)
                     /locus_tag="OO161_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(7971..8519)
                     /locus_tag="OO161_003053"
     CDS             complement(7971..8519)
                     /locus_tag="OO161_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5464037.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8519..9226)
                     /locus_tag="OO161_003054"
     CDS             complement(8519..9226)
                     /locus_tag="OO161_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5464038.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9241..9918)
                     /gene="ydjY"
                     /locus_tag="OO161_003055"
                     /pseudo
     CDS             complement(9241..9918)
                     /gene="ydjY"
                     /locus_tag="OO161_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
     gene            complement(9923..>10063)
                     /locus_tag="OO161_003056"
     CDS             complement(9923..>10063)
                     /locus_tag="OO161_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5464039.1"
                     /translation="RFILQLCLAGLALFILVQLAKLYARHKHVDLSASRRSPLTHPKN
                     EG"
BASE COUNT         2403 a         2739 c         2597 g         2324 t
ORIGIN      
        1 gccacgcaag caccttaaaa tcaccatcat acactaaatc agtaagttgg cagcattacc
       61 ggcatatctg ctgctatcgc agaaagttaa cggatgctga acttgaagcg tgttatcgtt
      121 acgacaagca gttttgggag cactacagta agaaatgccg ttggcccata agaaatgttc
      181 cctggggagc atcatccgtt aaatactcat aaacgcctga aattttgccg ggggcgctgc
      241 gcttgcccgg cctacattag agcgtaactg caatgaattg tgcccatttg taggcctgat
      301 aagcgtagcg ccatcaggca tttacaactt aaatcacacc ctgcgccagc atcgcatcgg
      361 caaccttcac aaaaccggca atgttcgcgc cctgcacgta gttggtttgc tcaccttcac
      421 caccatgctc aacacaggca tggtggatat ccagcatgat gtgatgcaaa cgtgcgtcaa
      481 ctttctcggc tttccagccc aggcgcgcag cgttttgcgc catttcaagg cctgacgttg
      541 ctacaccgcc agcattagcc gctttacccg gtgcaaacag tacgcctgcc tgctggaaca
      601 gttcagtcgc ttcgatggtg gtcggcatat ttgccccttc ggcgacggct ttaacgccat
      661 tggcgataag ctgatgcgcg gcgtcaacat ccagttcgtt ctgggtggcg caaggcaggg
      721 cgatatcaac cggtacagac cacggctgtt ggccttcgag atagaccaga ccaaattctt
      781 tggcgtaatc tgccactcga ccatcgcggc tggctttgat ttcgataaga cgtgccagtt
      841 tctctttcgt gaatccgctt tcatcaacta cagtgccgct ggagtctgac gcagtgatca
      901 cacgagcacc aaattccatc gctttttcga tagcgtactg ggcgacgttg ccggagccag
      961 aaacggaaac gcgcatccct tcaaaaccca taccgtggcg ttttaacatt gcttctgtga
     1021 aataaaccag accgtagccg gtagcttccg ggcgaataag actaccgcca aatgaaaggc
     1081 ccttaccggt gaagacgcag gcggtattgt tggagagctt tttcatcatc cccgccataa
     1141 agccgacttc acgaccacca acgccgatat cacctgccgg aacgtcggta tccgcgccca
     1201 gatgacgata aagctcggtc atcagcgcct ggcagaaacg catcacttcg ctttcgcttt
     1261 tacctttcgg gtcaaaatcg ctaccgcctt taccaccgcc catcggcagg gtagtcaggg
     1321 catttttgaa ggtttgttcg aagccgagga atttgagaat ggagaggtta actgacggat
     1381 ggaagcgcat accgcctttg tacgggccga tggctgagct gaactgcaca cgccatgcac
     1441 ggttgacctg tacctggttg cgatcatcaa cccataccac gcgaaactgg atcacgcgct
     1501 ccggttcaac cagacgctcc agtaatgaca tctggcgata ttttggattt tgttcaagaa
     1561 aaggccagag tgtggtcatt acttcacgaa cggcttgcgc gaactcggtt tgattcgggt
     1621 cgcgcttttg gacatggttg aggaatgact ccagagaata tgtctgatcc atagatataa
     1681 aacccttata tattaatacg attgtgctta tgttgtttat gtcatgtgct tttgcagttt
     1741 tcgactatac catcaagaat gtgacggtaa gcaagaaaaa aatgacccag gaacgcaaat
     1801 taataacgag agtaatctca taaccaaatg cgataactca gacgctaaag ttttgccacc
     1861 cggctaccgt tatcagttgc agagacaata acgttaagga gttccctgtg agtcgagcat
     1921 tgttcgccgt agtattagca tttccattaa ttgcactggc taacccgcat tatcgtccgg
     1981 acgtggaggt aaacgtaccg ccagaagtgt tcagttcagg cgggcagtca gcgcagccct
     2041 gtactcagtg ctgcgtttat caggatcaaa attattctga aggggcggta attaaagccg
     2101 aaggaatact gctgcaatgc cagcgggatg acaaaaccct tagcactaat ccgctgctct
     2161 ggcggcgcgt aaagccataa acgcctctaa tagtggaatg tcagtagggg ccagcggata
     2221 ttgcagcgcc tcttcaggtg agcaccagac cagcgcctga tgttcatgtg cctgtaacgt
     2281 cccgtggaag tcgggtacgt gccaggcatg aagatggata atccggcccg aaacttctcg
     2341 ctgatggctg gcaacatact cacccacagt tgcttcgatg cccaattctt cgtttaactc
     2401 acgcaccagt gcctgctgct ggctttcatc cagctcgact ttaccaccgg caaactccca
     2461 taatcccgcc tgatcgctct gggcggggcg ttgcgcgagt aaaattttgc catcacgttc
     2521 aatgatggcg gcaacaactt caatcatttt cataaattac acacgggcag ataagcgaaa
     2581 aatgccataa tatccgcact gttgctcaat cagaaaggct ttatatttaa ggagtgcctg
     2641 tgctagaccg ccatcttcat ccccggatta aaccgttgtt gcatcagtgc gtgcgggttc
     2701 ttgataaacc gggcattacg ccagatggtt taacattagt gggatttgcc atcggcgtgc
     2761 tggctctgcc gtttctggcg ctgggctggt atctggcggc gctagtcgtc attttgttga
     2821 acaggctgct tgatggtctg gacggtgcgc tggcgcggcg cagagagctt accgacgcgg
     2881 gcggctttct tgatatttct ctcgattttc tcttttacgc gctggtgccg tttggcttta
     2941 ttcttgctgc accggagcaa aatgcgctgg caggcggctg gttgttgttt gcgtttatca
     3001 gcacggcagc agttttctcg cctttgctgc actggcagca aaacatcaga ttgataaccc
     3061 cggttatgca cataagtcgt tttactatct ggggggatta accgaaggca cagaaacgat
     3121 cttactgttt gtgctgggat gtttattccc tgcatggttt gcgtggatct tcggtgcgtt
     3181 gtgctggatg acgaccttta cgcgggtgtg gagtgggtat ctgacgctga agtcgctcca
     3241 gcgtcagtaa ttgcaatgtt atttgctact gtccgggccg cgttcaccgg ttgctaccgg
     3301 attttttgga tcgctgctcc attcgtacca gccgccgtca tacacggaaa cattcttcca
     3361 acccatggcg cgtgcgtaca taaaggtttc ggacgcgcgc cagccggtgc cgcagtagaa
     3421 tgaaacttgc tgctctggtt tgatattcca cgctttccac atagcggtaa tatcatcggc
     3481 gctgcgcatg gtgccatccg ggttatgaaa atcttccata tgcgtcgagt cgctaccagc
     3541 gtgtccccaa cgtgctccgg ctatttcacc ttttggttta atatagctgt aaccgctggt
     3601 cgtaccgata aattctggcc acgaacgaat gctcaccagc gatgcatcct ggcgatgcag
     3661 cagtccacgg gcctgttcca tatcgagcat caactgcggt tgtgccggga tcttcacgcc
     3721 gaaatccggt tccgctttca ctttcggtgg cgttcctcgc tcaacaggca gtcccgcgtc
     3781 ggaccaggtt tgccagccgc catccagcag acgcacatct ttcacgcctg cgtagagcat
     3841 aatttgcgcc acacgcgctc cagcgtatac gtcacgcccg tacagaatga ccgtggtgtc
     3901 atggcgaatg ccgtgttttg ccagcatcgc ttttagttgt tcatcagaaa ctttgttcca
     3961 cagcggttcg ctttccactt cgttggtatc gatgtagtca gcaccgggaa tatggctgat
     4021 aaggtaaagc ttaggagcgc cctaggccgc ttcaatgact ttccagtcac cggcaggttt
     4081 cgccgtaacc tctttacctt gttgcaggtc gtgcagccat tgcggataaa ccagctgctc
     4141 aaagtgcggc agtttttgta gacgggaagg ttcgcttagc gcgtcactca gaatggagat
     4201 atgcgtaaaa cctgcttttt gcagtcgcgt tttgacggcg tcgacatctt tgtcattacc
     4261 gtacagcgcc accggggcat cggctttcag gttatgttgc ttgatccacg cgttgagctg
     4321 ttcggtgctc attttgtcga gccagctggc agagaggttt aaggcggcag gttcatgacc
     4381 agaagggcca tttaaggttt gtggccagcc gttataaaac gcgctggggc gagtatctat
     4441 cgctttgcca ttttgttgtt gaagctggtc aagtgtaaga ggcttcgcca gttcagcggc
     4501 ccacgaagaa gcgcaagcca gccctaaagc cattgccagc gcggtcattt gagaaacacg
     4561 tttcatcgaa aatcccgaca ttgtagtttt gagtgcgcca ttctgaactg acgcattgag
     4621 aaaaactttg cgttatcgca gtttgttgta attttctgac cactgcgcca tatccagaac
     4681 agaactatca gcaggaacat cctggagatc gtgcgttacc tgaacgacgg ggatcgccag
     4741 ttcgcgaact tcgctgaaca cccactggcg aaaattatcg cgcagagcca catcaagacg
     4801 gctgaatggc tcatccagga gtaacgcttt tggctgagcg agaagggcgc gcagcagggc
     4861 cacccgcgct cgctgaccgc ctgataatgt cgcagggtcc tggtgataag tttcagccag
     4921 tcccgctcga tcaagcgcgt ctttcacggc attgcgcctg gcagtcccct tgagcgtcga
     4981 cggtagcgcc agcagtaaat tttgcccgac gctgaactgg tcaaataaca gtgcatcctg
     5041 gaaaagaatg ccaatctgac gctgtgcggt gggtagcatg tcaatccgtt gctcattgag
     5101 ccatagctca cctgtacaag aaaactgtcc agccagtgca ccaatcatcc atgaaaacag
     5161 agtggatttt ccacagccag acggccccat taacgtgacg atgtcacctt tatcaaccgt
     5221 aaagttaacg tttgtcagca agcggctttc tggtaaacgt agcgaaacat ttttcacgca
     5281 gagcattagc ggagtccttg tctgacataa cctacccatt ttgcgactaa cgcggtcagg
     5341 gcaaaaataa taagcggtaa caacagttgc cataaagcct gggcggcgag aataccgttg
     5401 ctgccgccgc tgcttaatgc caccgcctca gtggtgagcg tcggaaaacg ccccgcgccc
     5461 agccacagcg tcggcatata ctgcgcaata ctgactgaaa atcccaccgc gaaggcaatc
     5521 agcacagggc gcaacatgag tgggcatttc acgtaaaaga agattttggc ccgcgaccag
     5581 cccagtgttt gcgcaatcaa aattaaccgt gaatcaatgc gctgccaggc gggttgcagg
     5641 ataaacagca tccacggcat tacccacagc agatgccccc agaccaccgc cgtccagctt
     5701 ccatccaggt tcagccatag cgccagcgtg tactggcccg ccaccagtgg cagagcgggc
     5761 aatagaatgg gcaaccatag ccacaactgg cgatgctgtg gcccccattc cagccacagc
     5821 agtaacagga gcaaagcgat gaatgttgct accagcccca ttgtcagact gttgatgagt
     5881 gcttcactat tgatcgtcga ctgatccgcg aggatcgcca gcagaaccac acacagcaca
     5941 ccggttaagg gtaaaaaact cgccagcgta gtgcctggta ataaaggtgt ggcaggcatt
     6001 gcgaatgcca tctacgcggg gaatagtgcg ccgccagctg cgccatagca gatagctcag
     6061 caaaacgtag gcggcgagta acagcatcag cagcagactg gcgagtgcgc ctttcgtttg
     6121 ttgatccgcg tcgccctgag ttaaccactg ccagctaatt actgccagcg tcggtggatt
     6181 acctggccca agaataatcg ccacatccac gaccgaaagc gactaggcaa caatcgccag
     6241 catcgccatt gccagcgaag gcgcgacgga gggcaacagc agccagttca ggcattgcca
     6301 gcggctgtag cccagcgaat ccagcacaat gacctgctgc aacagccgtt tttcgctcaa
     6361 tactgccgct aagatccaca gcagaaatgc gctttctttc actgcaaggg tgaggcccag
     6421 accgatgcca aatcggtcca ttggcggagt gaaatacggg aaatagtcat atagcagccc
     6481 tccgtcagca aagagcagca gggcgctggt ggcaaaagcc acatggggaa tggcgagcag
     6541 ccacggcaga cgggcgcaca tacgctgcca tttcggccca ggccatagcg ccacaatcac
     6601 cagcagggca atcaacaatg ccccgaccgc cgcgatggtt gtcgacacca gcgttgccag
     6661 taatgcatgc ggtaactgtg gatcggcaaa taacgcctgc cagtgtgtca acgacaacgc
     6721 aggcgatatc agcgtgagag ctgccgggat cagcggtgca taaatcaccg ccaccatcgc
     6781 ccacagcaaa aagattaatg cgtaccgtaa cggcgtaacc attcttgttc cagtgcattc
     6841 acccatcctg cgtgcggttc agccagtacc ggcggcagat cctgcggcat tcttgattgc
     6901 aatatttcgc gctgcccgtc aggcagtttt tgcggatcga gaacagaagg atcgccccag
     6961 acaacgggat ctgctttacg cagttgcgca tcgggtgaga gcagaaaatt ggcaactacc
     7021 ttcgccgcag cactggcatt cgcgttggct ggaatggtga cgaaatgcac gttgccaatc
     7081 atcccctcgc gaaagccaaa actgtaactg cttgccggca aatcgccgct ggcgattttt
     7141 tgctgcgcat gcgcggggtt aaaggtcagc gacaggcgca atgtgccggc tttcagcaga
     7201 gcatccatcc gcgcgggcga aggcgggaaa tcctttcctt cgcgccacaa atacggatgc
     7261 agtgcatcaa gatattgcca caagggcgca gtgacacggg cgaaagtcgc atcgtccggc
     7321 gcttctttta atgcggcggg atctggcgtc agcataatca gcaactgttc aagaaacgcc
     7381 gtgccggtaa agtccggtgg gcgcggataa gtaaccgtgt cgggattggc tttagcaaac
     7441 tccagtaagg cttgcggcgt ttgtggtggc tgtggcgtaa catcgcggcg ggcgataaac
     7501 gtcagttgtg cgccgcccta gggcgattcc gcaccttgtg tcggcactga aaaatcttcc
     7561 cgcaccggca gctgtgtgtc gacatagcgc cagttgggca gagtctccgc ccagcccgtt
     7621 tgtaataaat tggcctcttt taaggtgcgg aagttttcgc cgttcaccca gagcagatcc
     7681 accgagccgc ccgttttacg accggcagca gcttcggtct gaatgcgctt caccgcgtct
     7741 gcggcatccg cgagacgaac aatcttcagg tttatagcgt aatgggtttt catctcgccg
     7801 ctaacccagt cgaggtagcg gttaattgcg gtatcgccgc cccaggcatt aaactagacg
     7861 gtttgccctt tggcctcatt tttaatttcc tgccagtcac tggcatgtgt tgccaaagaa
     7921 aacagcgata acaatcccag caaccaccca caatggcgca tcttctctcc ttaagaacgt
     7981 ttttccggaa tacggcacag cccctgagcg ggaatgtcca tggcagaatt aaaacgagcc
     8041 gacaggtttt gcaggccgat caatgccgtc agttcggtga gcgcctgagc gtcaaaatgc
     8101 gcagccagtc gggtacgcag ggcatcatcg actgttggcg gtgtgacgct tgcggcttcg
     8161 gcgtactcca gcgccagccg ttcttcatcg ctaaagagcg ggctttgccg ccagtcagcc
     8221 actgccagca gtttatcggt gctgccggta cgctcggcga ctttcatact ggtgatatcc
     8281 acacaaaact cacacaggca catttgcgca atgcgcgcgc tgacaagcga tcgcactacc
     8341 ggatcgagcg gtgagcgttt gcgctccagc cacccaacaa acattgacac caaataaaag
     8401 ataaacggga tccgccccca ccagcgaatc gggcttagca cctcgccgta atggcgacga
     8461 tgcagccacg cctgcggacg taaaatgaaa ggaattttgc taagcggcgg taaacccatc
     8521 agtcattcct cttctggcgc tcaagccata ttttcttcgc cataaaaatc accacggtca
     8581 gggcaaacag aataaacagt ccggtgacaa accagaaagt accgcctgtt aacatgctgc
     8641 ccgcccagga ataaacaata gtcgccggta attgaccaag tccggtggcg ataaaaaacg
     8701 agcgaaaacg tattgaagtc agaccggcag catagctgat tggatcgaaa gggacaaaag
     8761 gcaataaccg acagaccagg atggtgtgtt tgccgtagcg agtgaaaaag ccgtccatac
     8821 tgtcaagcac ggttttgccg gttaattttt ccaccacttc gcggcccatc actctggcga
     8881 taaaaaagca cagcgccgcg ccggccatcg aactggtcca cgacagcaag ccgccccaga
     8941 acgcgccaaa cagcgacgca ttggcaaagg tgatcaaaaa cgcaggtagc ggtgcagcaa
     9001 tggcctgtaa aatcatcaac aagaacgaga caactgccgc cagtgcgccg taagactgaa
     9061 taaagcgttc tataccctgt tggtccacgg cggcaaacgc cgcaacgctg cgattgataa
     9121 actcatggac gccgggaagc agcgcccatg cgagcagcat agcgaacaac aggatgatga
     9181 gggttatacg gtagtaccag attttacgcg attgcatctt catcatcctt tatcctggct
     9241 ttattcggtg attttaaagg taaccgttgc cagcgtgtta tccgccggga gaactgaggc
     9301 attgcctttg aatttcactt cgccgcgttt ttccaccgca ccataagtgt atgttgcatt
     9361 gctgacgatg ccgaccgggc agctatccag acacaccagt caacccgttt tcttctcttc
     9421 tgctgccgtt aaattaccgc caaagcgcat gtccagtttc ttgccattac tgtcaacaat
     9481 cacttcatcg aaggaatacg cttttgccgc cccttgccag ttgaccgaaa tatccagttt
     9541 gctgcctgtg acatgagtcg tttctttatt atccatcgtc atgttttcgc ccggcgtacc
     9601 acctgcctct ttcagggctt cataaaatgc tttcggggtc gcataagcca taaacagcga
     9661 tttatgtccg ttggagccat ctttaaacac aataccgtga cgagtgtcgt cggtgaggaa
     9721 acgaccatta atttgcacca gcatggttac cgtatggtta ttggcatcga cgcgcagcgg
     9781 ttggctgttc gaaagtccgt cgtattccac ttttgccgcc gcattctctt tttgatcgca
     9841 gccgctaagc ccggcaacag ccagtaagca gagtgcagcc agtccttttt tccatgacac
     9901 tgaataatgt tgcgacatcg ttctatcctt cattttttgg gtgagtaagc gggctgcggc
     9961 gcgaagcaga cagatccaca tgtttgtgtc gggcgtagag ttttgcgagc tggacgagaa
    10021 taaacagcgc caggcccgcc agacagagtt gtaaaataaa gcg
//
LOCUS       DAJTNA010000141        10004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTNA010000141 DAJTNA010000000
VERSION     DAJTNA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10004
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.142"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(272..1636)
                     /gene="tdcG"
                     /locus_tag="OO161_003057"
     CDS             complement(272..1636)
                     /gene="tdcG"
                     /locus_tag="OO161_003057"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5464040.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPSPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLVYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAIYAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1708..2097)
                     /locus_tag="OO161_003058"
     CDS             complement(1708..2097)
                     /locus_tag="OO161_003058"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5464041.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(2111..4405)
                     /gene="tdcE"
                     /locus_tag="OO161_003059"
     CDS             complement(2111..4405)
                     /gene="tdcE"
                     /locus_tag="OO161_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5464042.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNVQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4439..5647)
                     /gene="tdcD"
                     /locus_tag="OO161_003060"
     CDS             complement(4439..5647)
                     /gene="tdcD"
                     /locus_tag="OO161_003060"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5464043.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEILMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDRVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5673..7004)
                     /gene="tdcC"
                     /locus_tag="OO161_003061"
     CDS             complement(5673..7004)
                     /gene="tdcC"
                     /locus_tag="OO161_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5464044.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(7026..8015)
                     /gene="tdcB"
                     /locus_tag="OO161_003062"
     CDS             complement(7026..8015)
                     /gene="tdcB"
                     /locus_tag="OO161_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5464045.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(8114..9052)
                     /gene="tdcA"
                     /locus_tag="OO161_003063"
     CDS             complement(8114..9052)
                     /gene="tdcA"
                     /locus_tag="OO161_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5464046.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            9241..9558
                     /gene="tdcR"
                     /locus_tag="OO161_003064"
     CDS             9241..9558
                     /gene="tdcR"
                     /locus_tag="OO161_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5464047.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQEKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRT"
BASE COUNT         2692 a         2514 c         2278 g         2520 t
ORIGIN      
        1 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
       61 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
      121 aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt
      181 aatcgcaata gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt
      241 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
      301 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
      361 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg
      421 cgtttactgc tttcacggca taaatggcat tacgttcaat gcacgggatt tgtacctgtc
      481 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
      541 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg
      601 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
      661 acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg
      721 gacgacggaa cttatcgtaa taaaccagca ctgccggaat aatgccgcac gcaccgttag
      781 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
      841 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct
      901 gacgacgcag cgctacggca cgacgcggca cattgagcgg actaggcagc acgccttcag
      961 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt
     1021 caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
     1081 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
     1141 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
     1201 cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc
     1261 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
     1321 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
     1381 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa
     1441 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccatac agatcgacca
     1501 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
     1561 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
     1621 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
     1681 ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
     1741 cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc
     1801 ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
     1861 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
     1921 cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc
     1981 ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
     2041 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
     2101 tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac
     2161 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
     2221 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattatgttg acgttgaggt
     2281 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
     2341 tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
     2401 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
     2461 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
     2521 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
     2581 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
     2641 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
     2701 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
     2761 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
     2821 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
     2881 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
     2941 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
     3001 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
     3061 agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
     3121 ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca
     3181 gatcgtcatt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
     3241 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
     3301 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
     3361 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
     3421 caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca
     3481 gttcctgtgc ctgctgctca ttgagtacgc cagctttaaa gtcgcgctca atgtagatat
     3541 cgaggaacga tgccgtgcgg cccagcgaca tcgcgccgcc attttgcgat ttcactgccg
     3601 ccagataagc gaagtagagc cactgcaccg cttcttgcac attctgcgcc gggcgagaga
     3661 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
     3721 ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact
     3781 ggagatcggc aaattgcagt tcgcgttcac gtaccagata actgatgcca tacagcgcta
     3841 cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca
     3901 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
     3961 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cggccatagg
     4021 cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg
     4081 gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat
     4141 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
     4201 cgatacggat accttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg
     4261 cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
     4321 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
     4381 cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt
     4441 atgcaaattc tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt
     4501 tttcttcgtt agtcggaata acagcgcaaa tgacacgcgc attttcactg gaaacaattc
     4561 gctcaccaca ggagttagag cgattattca tttctgtatc aatctctacg cctaatacag
     4621 ccaaatgttc catgaccaga cgacgaatta agcttgaatt ctctcctatt ccgccggtga
     4681 atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc
     4741 ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt
     4801 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
     4861 ctacgcgttc caggtcaccc aagctctggt tggtttggct ggcgacccag gacatcgcac
     4921 caaaatcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca
     4981 ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca ttgccaagat
     5041 gcgccacaac caggccggag tcatcttccg ctagattcag caaagaatgt gcgcgctggg
     5101 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
     5161 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
     5221 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
     5281 aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgacct
     5341 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
     5401 ttaaggccac tctgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
     5461 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
     5521 cattttccga gttaataccg tcggcaatac ctgacattaa tatttcacag tcgctggcat
     5581 cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
     5641 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
     5701 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
     5761 agagacggcg ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc
     5821 ggtgcgccca tggcttcaat aaggtcgagg atgttcgggt tggcgtaggc aacaacccag
     5881 gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt
     5941 ttgtcgcctt tataaccgaa cttcagaatc agaccattca agccttccag cgtccccaga
     6001 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
     6061 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
     6121 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaac
     6181 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
     6241 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
     6301 ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa
     6361 atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
     6421 tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc
     6481 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
     6541 aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc
     6601 ggtgcaaatc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
     6661 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
     6721 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
     6781 agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
     6841 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
     6901 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
     6961 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
     7021 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
     7081 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
     7141 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
     7201 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
     7261 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
     7321 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
     7381 ttttctgact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
     7441 ataccagcaa ttaaaccacc accaccaatt ggcacaatca cgttatcgac atcatagaga
     7501 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
     7561 ggtgggataa aaatacgacc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
     7621 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
     7681 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
     7741 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
     7801 gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
     7861 tgcatatttt caaacttcag aaatatttca cctttgcaac gttcactaaa atagttggag
     7921 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca
     7981 tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
     8041 atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
     8101 taaaaacaaa taactaacca acttctatta atagccttcg tctacaccca ttataagatg
     8161 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
     8221 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
     8281 gatttgaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
     8341 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
     8401 catttctttg taacgtagta agaagctcgc tgtagtaccc catattggtt tgtggcaaca
     8461 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
     8521 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
     8581 tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
     8641 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
     8701 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
     8761 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
     8821 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
     8881 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
     8941 cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga
     9001 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
     9061 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
     9121 gaccactcac aaattaactt tcattacttt ccagggtatc gttattacaa ttaaatatct
     9181 ttaaatatca acaagttaaa gcataaaaat cagcataaac cctgattttt atcaaaaaaa
     9241 atgacgggga taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa
     9301 aaggggctga gaccatattt caagcaatta ccggataatt accaggaaaa atttgagtta
     9361 aatttaatga gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact
     9421 gcggcacgtc acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat
     9481 tttaaaattt cgaatacagg tattgatcat ctcgaacaat tgattaacgt caactttttc
     9541 tcttctgaca ggacgtgatt ttgtgaatgc aatcgttttc cataaattct tctcccctca
     9601 taggcgacga atagcatttt gtgttgagga tcacaaaacg aataattgct ggtcgccgcg
     9661 ataaggtcag acgaagtcaa caaggggaat tttcactgtg ctttacatca gcgtaggaca
     9721 cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag cgttttttga tagtgcgtga
     9781 gtgtcgtgcg aggaaaataa gcatctatta ttgtttgtca tgacgacaag gcaggcatca
     9841 atgaaaggct tcccaattgc acatattttt cacccttcaa tcccgccaat gcacgctgtg
     9901 gttaacaatc acaatagaaa tattgattat tggacggtaa aaagaaagtt tgcggaaatt
     9961 gtctccacca atgacgttaa aaaaatttac agtataagta atga
//
LOCUS       DAJTNA010000142         9914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTNA010000142 DAJTNA010000000
VERSION     DAJTNA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.143"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..565)
                     /locus_tag="OO161_003065"
     CDS             complement(<1..565)
                     /locus_tag="OO161_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5464048.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     YAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGH"
     gene            complement(784..1761)
                     /gene="epmA"
                     /locus_tag="OO161_003066"
     CDS             complement(784..1761)
                     /gene="epmA"
                     /locus_tag="OO161_003066"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5464049.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGSETLAEVIAFSVDRA"
     gene            2086..3894
                     /gene="frdA"
                     /locus_tag="OO161_003067"
     CDS             2086..3894
                     /gene="frdA"
                     /locus_tag="OO161_003067"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5464050.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVPIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3887..4621
                     /gene="frdB"
                     /locus_tag="OO161_003068"
     CDS             3887..4621
                     /gene="frdB"
                     /locus_tag="OO161_003068"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5464051.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIEHD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4632..5027
                     /gene="frdC"
                     /locus_tag="OO161_003069"
     CDS             4632..5027
                     /gene="frdC"
                     /locus_tag="OO161_003069"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5464052.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5038..5397
                     /gene="frdD"
                     /locus_tag="OO161_003070"
     CDS             5038..5397
                     /gene="frdD"
                     /locus_tag="OO161_003070"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5464053.1"
                     /translation="MINPNPKRSGEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5460..6590
                     /locus_tag="OO161_003071"
     CDS             5460..6590
                     /locus_tag="OO161_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5464054.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAACQILNALQ"
     gene            6679..7212
                     /gene="blc"
                     /locus_tag="OO161_003072"
     CDS             6679..7212
                     /gene="blc"
                     /locus_tag="OO161_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5464055.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7209..7526)
                     /gene="sugE"
                     /locus_tag="OO161_003073"
     CDS             complement(7209..7526)
                     /gene="sugE"
                     /locus_tag="OO161_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5464056.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="OO161_003074"
     CDS             complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="OO161_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5464057.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="OO161_003075"
     CDS             complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="OO161_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5464058.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8142..8708)
                     /gene="efp"
                     /locus_tag="OO161_003076"
     CDS             complement(8142..8708)
                     /gene="efp"
                     /locus_tag="OO161_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5464059.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8750..9799
                     /gene="epmB"
                     /locus_tag="OO161_003077"
     CDS             8750..9799
                     /gene="epmB"
                     /locus_tag="OO161_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5464060.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
BASE COUNT         2543 a         2555 c         2518 g         2298 t
ORIGIN      
        1 aatgcccacc atttaataac aaaatagtaa tgcttactaa cagcaatact aaattcagac
       61 acattactgc aataccgccc accgcagtaa tgcgggcaat tttattaatc ccctttgaag
      121 caacgacggt gaccagaatc atccatgcta ctgccagcag accaaccacc tgcgtaggct
      181 ccagtccggc aatacgccag tgctgggtca tgtcgctacc atagaggaat gttgagaacg
      241 gtacccaaac tttcgcgtag gtactcacca tccagatgat ataagaggaa aaccacataa
      301 acgtaccaat gaaggcaaaa cgtggtccga cactattatt catccaggaa tagataccac
      361 cttcttcttt gcgataagca gctcccattt cagccatcat taaggcgaat ggaataaaga
      421 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      481 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      541 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      601 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      661 atactcggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      721 catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      781 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg agcccagcgc
      841 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      901 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      961 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
     1021 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
     1081 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
     1141 ttctttgcca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
     1201 gtcttcttcg gtatcagcaa cgttactcaa atccagtttc gctgcgactt cccgcagttg
     1261 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
     1321 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1381 caaccggtac atatcatagt gcggtcgata ccactcaagc atagtgaact cagggttgtg
     1441 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1501 ggcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1561 ctgcgaatgc ccggggccta cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1621 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcag caaagaaacg
     1681 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1741 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1801 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1861 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1921 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1981 gttgtaccta taaaggagca gttgaatagc gttcgcagac cgtaactttc aggcacttac
     2041 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     2101 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     2161 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     2221 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     2281 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     2341 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtgggggtg cccatggagc
     2401 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2461 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2521 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2581 ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt gccgatccgt
     2641 gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg tttatcgtta caacaccaac
     2701 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2761 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2821 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2881 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2941 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     3001 tccacgccgc gtggcgatgt ggtttatctc gacttgcgtc acctcggcga gaaaaaactg
     3061 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     3121 aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat cgaaaccgat
     3181 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     3241 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     3301 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagaggca
     3361 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc
     3421 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctag caatggaaga aggttgcggt
     3481 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3541 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3601 accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3661 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3721 gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3781 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3841 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
     3901 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3961 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     4021 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     4081 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     4141 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacacg atctggtggt
     4201 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     4261 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     4321 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4381 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4441 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
     4501 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4561 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4621 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4681 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4741 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggaagcct
     4801 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4861 tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4921 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4981 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     5041 attaatccaa atccaaagcg ttctggcgaa ccggtattct ggggcctctt cggggccggt
     5101 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     5161 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     5221 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     5281 cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc
     5341 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtatcgttac aatctaacgt
     5401 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5461 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc
     5521 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5581 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5641 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5701 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5761 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5821 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcagatg ccggatgaag
     5881 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5941 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     6001 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     6061 accatacgtg gattaatgta ccgcccgcag aagaaaagaa ttacgcctgg ggatatcgcg
     6121 aaggcaaggc agttcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     6181 cgaccattga agatatggcc cgctgggtgc aaagcaattt aacccccctt gatatcaatg
     6241 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     6301 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     6361 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     6421 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     6481 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     6541 ccaatccagc gagagtcgcc gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg
     6601 ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa
     6661 accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt
     6721 ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg tgacagtagt gaataatttc
     6781 gacgctaaac gctatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa
     6841 cgtggactgg aaaaagtcac cgcaacatac agtctgcgtg atggcggcgg cctgaatgtc
     6901 atcaataaag gctataaccc tgacagagaa atgtggcagc agagtgaagg gaaagcgtac
     6961 tttaccggcg caccaacacg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc
     7021 ggttataacg tcattgcact tgatcgggaa taccgccatg cgctggtttg cggcccggac
     7081 cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag
     7141 atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag
     7201 cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga
     7261 ctcgccaggc gcatcgggtt agcggactca ccgagcagca caatgccggt gatagccgcg
     7321 ccgactgcgc caatacccgt ccacacggca taagccgtcc ctactggtaa cgatttcatc
     7381 gcccaggcaa gtagcgccat actgacaatc atcgccgtca cggtaataac actcggcgtc
     7441 aaacgactaa agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca
     7501 gcaataacta agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg
     7561 aaaagacgcg tttgcaggtc gtcctgcaac gctgatggtt gaacggatat tttgccaggt
     7621 ggcaggggaa tttcaactat taccttagtg actatcgcag aaaaaacaaa cggcacgaca
     7681 cagctgtcgt accgttattg cttattgcta ttgctgcgct ttcgttgctg cgccagaaat
     7741 cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa
     7801 tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc
     7861 ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc
     7921 caaaaatgac ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga
     7981 gagatggagt tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt gttacagccc
     8041 gtgagcagcg tactggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg
     8101 aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg
     8161 tattcaccag agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaacc
     8221 ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct
     8281 ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag
     8341 atcggctgac cattccacag agttacgata cactctgcct gatccagcag ccatttagcg
     8401 ttatcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag
     8461 tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct
     8521 tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc
     8581 agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc
     8641 gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac
     8701 gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat
     8761 tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac
     8821 cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg
     8881 acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga
     8941 gaaaggcaat ccggacgatc ctcttttgcg tcaggtactt acctcgcaag atgagtttgt
     9001 cgtcgcgtcc ggattctcca ccgacccact ggaagaacag cacagcgtag tgcctggttt
     9061 gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg
     9121 ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg
     9181 gcagactgca cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg
     9241 cggcgatccg ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc
     9301 catcccgcat atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg
     9361 catcacagat gcgctggttg aacgcttttc acattctacg ctgcaaatcc tgctggtgaa
     9421 tcacatcaac catgccaatg agatagatga aacattccgt caggcgatgg ctaagttgcg
     9481 ccgggtaggc gttactttgc tgaaccagag cgttctgtta cgtgatgtga acgataacgc
     9541 acaaacgctg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtgatgc cgtattacct
     9601 gcatgtgctc gataaagtac agggcgcggc gcattttatg gtgagtgatg acgaagcacg
     9661 gcagattatg cgtgagttgc tgacattggt gtcgggatat ctggtgccga aactggcgcg
     9721 agaaattggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc agcagttact
     9781 gattaaacag ggaagttaaa cattaaaatt gcctgatgcg ctacgcttat caggcctaca
     9841 tcgtgcattg aaattgaatg gatttgaaat gaactgtagg ccggataagg cgtttacgcc
     9901 gcatccggca agag
//
LOCUS       DAJTNA010000143         9379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTNA010000143 DAJTNA010000000
VERSION     DAJTNA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.145"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..467
                     /gene="wcaE"
                     /locus_tag="OO161_003078"
     CDS             <1..467
                     /gene="wcaE"
                     /locus_tag="OO161_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5464061.1"
                     /translation="FHQDAANFVRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGCY
                     IYHSLPASHQAIFFPVSGLKKWRYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFS
                     MGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            483..1031
                     /gene="wcaF"
                     /locus_tag="OO161_003079"
     CDS             483..1031
                     /gene="wcaF"
                     /locus_tag="OO161_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5464062.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            1058..2178
                     /gene="gmd"
                     /locus_tag="OO161_003080"
                     /pseudo
     CDS             1058..2178
                     /gene="gmd"
                     /locus_tag="OO161_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            2181..3146
                     /gene="fcl"
                     /locus_tag="OO161_003081"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             2181..3146
                     /gene="fcl"
                     /locus_tag="OO161_003081"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5464063.1"
                     /translation="MSRQRIFIAGHRGMVGSAITRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            3149..3628
                     /gene="gmm"
                     /locus_tag="OO161_003082"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             3149..3628
                     /gene="gmm"
                     /locus_tag="OO161_003082"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5464064.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3625..4848
                     /gene="wcaI"
                     /locus_tag="OO161_003083"
     CDS             3625..4848
                     /gene="wcaI"
                     /locus_tag="OO161_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5464065.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAYTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4851..6278
                     /gene="cpsB"
                     /locus_tag="OO161_003084"
     CDS             4851..6278
                     /gene="cpsB"
                     /locus_tag="OO161_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5464066.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAAKRH
                     SPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYIRR
                     GEVSAGEQDTVAFEVAQFVEKPNLKTAQAYVASGEYYWNSGMFLFRAGRYLEELKKYR
                     PDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWSD
                     VGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAV
                     LIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPG
                     EGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKIPLD
                     LIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6471..7841
                     /gene="cpsG"
                     /locus_tag="OO161_003085"
                     /gene_synonym="manB"
     CDS             6471..7841
                     /gene="cpsG"
                     /locus_tag="OO161_003085"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5464067.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLVLAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEGRFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKDKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7977..9371
                     /gene="wcaJ"
                     /locus_tag="OO161_003086"
     CDS             7977..9371
                     /gene="wcaJ"
                     /locus_tag="OO161_003086"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5464068.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGRAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
BASE COUNT         2241 a         2385 c         2672 g         2081 t
ORIGIN      
        1 tttttcatca ggatgccgca aattttgtcc gtaagttaaa aatgcaaaaa gataacatga
       61 tgatcaccgg cgatgcgctg ctggattttg gcgacgggca taaaattaaa cgtagcgcca
      121 aaccgggctg ttatatttat cacagcctgc ccgccagtca tcaggcgata tttttccctg
      181 tatccggctt gaaaaaatgg cgttatgacc tggaatataa agtttcttcc gactacgcgc
      241 tggcagccaa aatgtataaa gccggttatg catttaaaaa actcaatggc ctggtgtctg
      301 aattttccat gggtggggta tcaaccacca ataatatgga attgtgtgct gacgcgaaaa
      361 aagtccaacg acaaatatta catgtacctg gcttttgggc tgaattatcc tggcatttac
      421 gccaacgtac tacctcaaag acgaaagcct tatataacaa agtctgaata taaggagaac
      481 caatgcaaga tttaagcggt ttctcggtgc cgaaagggtt ccggggcggc aacgctatta
      541 aagtgcaatt atggtgggca gtacaggcaa caatatttgc ctggtcgcca caagtattgt
      601 atcgctggcg ggctttttta ttacgtttat tcggggcaaa aataggaaaa aacgtagtta
      661 ttcgtccgtc agtaaaaatt acctatccgt ggaaattaac cttaggtgat tacgcgtggg
      721 tcggagatga ggtcaattta tataccctcg gtgaaataac cattggcgca cattcggtga
      781 tatcgcaaaa aagttattta tgcaccggta gccacgacca tgcaagtcaa catttcacca
      841 ttaacgccac gcctattgtg attggcgaga aatgctggct ggcaaccgat gtttttgttg
      901 cccctggcgt cacgatcggc gacggcaccg tcgtgggggc gcgaagcagt gtttttaaat
      961 cgcttccggc aaatgtggtt tgccggggga atcccgcagt ggtgatacgc gaacgcgttg
     1021 aaactgaata aattcaaaaa atacagagga ataagacatg tcaaaagtcg ctctcatcac
     1081 cggtgtaacc ggacaagacg gttcttacct ggcagagttt ctgctggaaa aaggttacga
     1141 ggtgcatggt attaagcgtc gtgcatcgtc attcaacacc gagcgcgtgg atcacattta
     1201 tcaggatccg cacacctgca acccgaaatt ccatctgcat tatggcgacc tgagtgatac
     1261 ctccaacctg acgcgcattt tgcgtgaagt gcagccggat gaagtgtaca acctgggcgc
     1321 aatgagccac gttgcgcttt cttttgagtc accggaatat accgctgacg tcaacgcgat
     1381 gggtacgctg cgcctgctgg aggcgatccg cttcctcggt ctggaaaaga aaacccgttt
     1441 ctatcaggct tccacctctg aactgtacgg tctggtgcag gaaattcagc agaaagaaac
     1501 cacgccgttc tacccgcgat ctccgtatgc ggtcgccaaa ctgtatgcct actggatcac
     1561 cgttaactac cgcgaatcct acggcatgta cgcctgtaac ggtattctct tcaaccatga
     1621 atccccgcgc cgcggcgaaa ccttcgttac ccgcaaaatc acccgcgcaa tcgccaacat
     1681 cgcccagggg ctggagtcgt gcctgtacct cggcaatatg gattccctgc gtgactgggg
     1741 ccacgccaaa gactacgtaa aaatgcagtg gatgatgctg caacaggaac agccagaaga
     1801 tttcgttatc gcgaccggcg ttcagtactc cgtacgtcag ttcgtggaaa tggcggcagc
     1861 acagctgggc atcaaactgc gctttgaagg cacgggcgtt gaagagaagg gcattgtggt
     1921 ttccgtcacc gggcatgacg cgccggcgtt aaaccgggcg atgtgattat cgccgttgac
     1981 ccgcgttact tccgtccggc ggaagttgaa acgctgctcg gcgacccgac caaagcgcac
     2041 gaaaaactgg gctggaaacc ggaaatcacc ctcagagaga tggtgtctga aatggtggct
     2101 aatgacctcg aagcggcgaa aaaacactct ctgctgaaat ctcacggcta cgacgtggcg
     2161 atcgcgctgg agtcataagc atgagtagac aacgcatttt tatcgctggc catcgcggaa
     2221 tggtcggttc cgccatcacg cggcagctcg aacagcgcgg tgatgtggaa ctggtattac
     2281 gcacccgcga cgagctgaac ctgctggaca gccgcgcggt gcatgatttc tttgccagcg
     2341 aacgcattga ccaggtctat ctggcggcgg cgaaagtggg cggcattgtt gccaacaaca
     2401 cctatccggc ggatttcatc taccagaaca tgatgattga gagcaacatc attcacgccg
     2461 cgcatcagaa cgacgtgaac aaactgctgt ttcttggatc gtcctgtatc tacccgaaac
     2521 tggcaaaaca gccgatggca gaaagcgagt tattgcaggg cacgctggag ccgaccaacg
     2581 agccttacgc cattgccaaa atcgccggga tcaaactgtg cgaatcatac aaccgccagt
     2641 acggacgcga ttaccgctca gtcatgccga ccaacctata tggcccgcat gacaacttcc
     2701 acccgagtaa ttcgcatgtg atccctgcat tgctgcgtcg cttccacgag gcgacggcac
     2761 agaatgcacc ggacgtggtg gtatggggca gcggtacacc gatgcgtgaa ttcctgcacg
     2821 tcgatgatat ggcggcggcg agcattcatg tcatggagct ggcgcacgaa gtctggctgg
     2881 agaacaccca gccgatgctg tcgcacatta acgtcggcac gggcgttgac tgcactatcc
     2941 gcgagctggc gcaaaccatc gccaaagtgg tgggttacaa aggtcgggtg gtttttgatg
     3001 ccagcaaacc ggatggtacg ccgcgcaaac tgcttgatgt gacgcgcctg catcagcttg
     3061 gctggtatca cgaaatctca ctggaagcgg ggcttgccag cacttaccag tggttccttg
     3121 agaatcaaga ccgctttcgg gggtaatgat gtttttacgt caggaagact ttgccacggt
     3181 agtgcgctcc accccgcttg tctctctcga ctttattgtc gagaacagtc gcggcgagtt
     3241 tctgcttggc aaaagaacca accgcccggc gcagggttac tggtttgtgc cgggagggcg
     3301 cgtgcagaaa gacgaaacgc tggaagccgc atttgagcgg ctgacgatgg cggaactggg
     3361 gctgcgtctg ccgataacag caggccagtt ttacggtgtc tggcagcact tttatgacga
     3421 taacttctcc ggcacggatt tcaccactca ctatgtggtg ctcggttttc gcttcagagt
     3481 agcggaagaa gagctgttac tgccggatga gcagcatgac gattaccgct ggctgacgcc
     3541 ggacgcgctg ctcgccagta acgatgttca cgctaacagc cgcgcctatt ttctagctga
     3601 gaagcgtgcc ggagtacccg gattatgaaa atactggtct acggcattaa ctactcgccg
     3661 gagttaaccg gcatcggcaa atacaccggc gagatggtgg aatggctggc ggcacaaggt
     3721 catgaggtgc gggtcattac cgcaccgcct tactacccgc aatggcaggt gggtgagaac
     3781 tattccgcct ggcgctacaa acgagaagag ggggccgcca cggtgtggcg ctgcccgctg
     3841 tacgtgccaa aacagccgag caccctgaaa cgcttgttgc atctcggcag ttttgccgtt
     3901 agcagtttct ttccactgat ggcgcaacgt cgctggaagc cggatcgcat tatcggcgta
     3961 gtgccaacgc tgttttgcac gccgggaatg cgcctgctgg cgaagctctc tggtgcgcgt
     4021 accgtgctgc atattcagga ttacgaagtg gacgccatgc tggggctggg ccttgccgga
     4081 aaaggcaaag gcggcaaagt ggcacagctg gcaacggcgt tcgaacgtag cggactgcat
     4141 aacgtcgata acgtctccac gatttcgcgt tcgatgatga ataaagccat cgaaaaaggc
     4201 gtggcggcgg aaaacgtcat cttcttcccc aactggtcgg aaattgcccg ttttcagcat
     4261 gttgcagatg ccgatgttga tgcccttcgt aaccagcttg acctgccgga taacaaaaaa
     4321 atcattcttt actccggcaa tattggtgaa aagcaggggc tggaaaacgt tattgaagct
     4381 gccgatcgtc tgcgcgatga accgctgatt tttgccattg tcgggcaggg cggcggcaaa
     4441 gcgcggctgg aaaaaatggc gcagcagcgt ggactgcgca acatgcaatt tttcccgctg
     4501 caatcgtatg acgctttacc cgcactgctg aagatgggcg attgccatct ggtggtgcaa
     4561 aaacgcggcg cggcagatgc cgtattgccg tcgaaactga ccaatattct ggcagtaggc
     4621 ggtaacgcgg tgattactgc tgaagcctac acagaactgg ggcagctttg cgaaaccttt
     4681 ccgggcattg cggtttgcgt tgaaccggaa tcggtcgagg cgctggtggc ggggattcgt
     4741 caggcgctcc tgctgcccaa acacaacacg gtggcacgtg aatatgccga acgcacgctc
     4801 gataaagaga acgtgttacg tcaatttata aatgatattc ggggataatt atggcgcagt
     4861 cgaaactcta tccagttgtg atggcaggtg gctccggtag ccgcttatgg ccgctttccc
     4921 gcgtacttta tcccaagcag tttttatgcc tgaaaggcga tctcaccatg ctgcaaacca
     4981 ccatctgccg cctgaacggc gtggagtgcg aaagcccggt ggtgatttgc aatgagcagc
     5041 accgctttat tgtcgcggaa cagctgcgtc aactgaacaa acttaccgag aacattattc
     5101 tcgaaccggc agggcgaaac acggcacctg ccattgcgct ggcggcaaaa cgtcatagcc
     5161 cggagagcga cccgttaatg ctggtattgg cggcggatca tgtgattgcc gatgaagacg
     5221 cgttccgtgc cgccgtgcgt aatgccatgc cgtatgccga agcgggcaag ctggtgacct
     5281 tcggcattgt gccggatcta ccagaaaccg gttatggcta tattcgtcgc ggtgaagtgt
     5341 ctgcgggtga gcaggatacg gtggcctttg aagtggcgca gtttgtcgaa aaaccgaatc
     5401 tgaaaaccgc ccaggcctat gtggcaagcg gcgaatatta ctggaacagc ggcatgttcc
     5461 tgttccgcgc cggacgctat ctcgaagaac tgaaaaaata tcgtccggat attctcgatg
     5521 cctgtgaaaa agcgatgagc gccgtcgatc cggatctcga ttttattcgt gtggatgaag
     5581 aagcgtttct cgcctgcccg gaagagtcgg tggattacgc ggtcatggaa cgtacggcag
     5641 atgccgttgt ggtgccgatg gatgcgggct ggagcgatgt cggctcctgg tcctcattat
     5701 gggaaattag cgcccacacc gccgagggca acgtttgcca cggcgatgtg attaatcaca
     5761 aaactgaaaa cagctatgtg tacgccgaat ctggcctcgt aaccaccgtc ggggtgaaag
     5821 atctggtagt ggtgcagacc aaagatgcgg tgctgattgc cgaccgtaac gcggtgcagg
     5881 atgtgaaaaa agtggtcgag cagatcaaag ccgatggtcg ccatgagcat cgggtgcatc
     5941 gcgaagtgta tcgcccgtgg ggcaaatatg actctatcga cgcgggcgac cgctaccagg
     6001 tgaaacgcat caccgtgaaa ccgggcgagg gcttgtcggt acagatgcac catcaccgcg
     6061 cggaacactg ggtggttgtc gcgggaacgg caaaagtcac cattgatggt gatatcaaac
     6121 tgcttggtga aaacgagtcc atttatattc cgctgggggc gacgcattgc ctggaaaacc
     6181 cggggaaaat tccgctcgat ttaattgaag tgcgctccgg ctcttatctc gaagaggatg
     6241 atgtggtgcg cttcgcggat cgctacggac gggtgtaaac gtcgcatcag gagctgtttg
     6301 tcggatgcgg cgtgaacgcc ttatccgacc tacggttcgg gtttgtagtc ctgataagac
     6361 gcggcagcgt cgcatcaggc aatgaatgcg aaaccgcggt gtaaataacg acaaaaacaa
     6421 aattggccgt ttcggtcagg gccaactatt gcctgaaaaa gggtaacgac atgaaaaaat
     6481 taacctgctt taaagcctat gatattcgcg ggaaattagg cgaagaactg aatgaagata
     6541 tcgcctggcg cattggtcgc gcctatggcg aatttctcaa accgaaaacc attgtgttag
     6601 gcggtgatgt ccgtctcacc agcgaaacct taaaactggt gctggcaaaa ggtttacagg
     6661 atgcgggcgt tgacgtgctg gatatcggta tgtccggcac cgaagagatc tatttcgcca
     6721 cgttccatct cggcgtggat ggcggcattg aagttaccgc cagccataat ccgatggatt
     6781 ataacggcat gaagctggtt cgcgaggggg ctcgcccgat cagcggagat accggactgc
     6841 gcgacgtcca gcgtctggct gaagccaacg actttcctcc cgtcgatgaa accaaacgcg
     6901 gtcgctatca gcaaatcaac ctgcgtgacg cttacgttga tcacctgttc ggttatatca
     6961 atgtcaaaaa cctcacgccg ctcaagctgg tgatcaactc cgggaacggc gcagcgggtc
     7021 cggtggtgga cgccattgaa ggccgcttta aagccctcgg cgcacccgtg gaattaatca
     7081 aagtgcacaa cacgccggac ggcaatttcc ccaacggtat tcctaaccca ctactgccgg
     7141 aatgccgcga cgacacccgc aatgcggtca tcaaacacgg cgcggatatg ggcattgcct
     7201 ttgatggcga ttttgaccgc tgcttcctgt ttgacgaaaa agggcagttt atcgaaggct
     7261 actacattgt cggcctgctg gcagaagcgt tcctcgaaaa aaatcccggc gcgaagatca
     7321 tccacgatcc acgtctctcc tggaataccg ttgatgtggt gactgccgct ggcggcacgc
     7381 cggtgatgtc gaaaaccgga cacgccttta ttaaagaacg tatgcgcaag gaagacgcca
     7441 tctatggtgg cgaaatgagc gcccaccatt acttccgtga tttcgcttac tgcgacagcg
     7501 gcatgatccc gtggctgctg gtcgccgaac tggtgtgcct gaaagataaa acgctgggcg
     7561 aactggtacg cgaccggatg gcggcgtttc cggcaagcgg tgagatcaac agcaaactgg
     7621 cgcaacccgt tgaggcgatt aaccgcgtgg aacagcattt tagccgtgag gcgctggcgg
     7681 tggatcgcac cgatggcatc agcatgacct ttgccgactg gcgctttaac ctgcgcacct
     7741 ccaataccga accggtggtg cgcctgaatg tggaatcgcg cggtgatgtg ccgctgatgg
     7801 aagcgcgaac gcgaactctg ctgacgttgc tgaacgagta atgtcattac tttccccctc
     7861 accctgacgc tctactgcct gcggagagaa gtgagcacgg aactttcttc tccccctcgc
     7921 ccctttgggg agagggccgg ggtgaggggc gaggcgacaa tagaacagga acaacgatga
     7981 caaatctaaa aaagcgcgag cgagcgaaaa ccaatgcatc gttaatctct atggtgcaac
     8041 gcttttcaga tatcaccatc atgtttgccg gactatggct ggtttgcgaa gtgagcggac
     8101 tgtcattcct ctacatgcac ctgttggtgg cgttgattac gctggtggtg ttccagatgc
     8161 tgggcggcat caccgatttt tatcgctcat ggcgcggtgt tcgggcagcg acagaatttg
     8221 ccctgttgct acaaaactgg accttaagcg tgattttcag cgccggactg gtggcgttca
     8281 ataatgattt cgacacgcaa ctgaaaatct ggcttgcgtg gtatgggctg accagtatcg
     8341 gactggtggt ttgccgttca tgtattcgta ttggtgcggg ctggctgcgt aatcatggct
     8401 ataacaagcg catggtcgcg gtggcggggg atttagccgc cggacaaatg ttgatggaga
     8461 gcttccgtaa tcagccgtgg ttagggtttg aagtggtggg cgtataccac gatccaaaac
     8521 cgggcggcgt ttctaacgac tgggcgggca atctgcaaca gctggtcgag gatgcgaaag
     8581 cgggcaagat tcataacgtc tatatcgcga tgcaaatgtg cgacggcgcg cgagtgaaaa
     8641 aactggtcca tcaattggcg gataccacct gttcggtgct gctgatcccc gatgtcttta
     8701 ccttcaatat tctccattca cgtctcgaag agatgaacgg cgttccggtg gtgccgctgt
     8761 atgacacgcc gctttccggg gttaaccgcc tgataaaacg tgcggaagac attgtgctgg
     8821 cgacgcttat tctgctgctg atctccccgg tgctgtgctg tattgcgctg gcggtaaaac
     8881 tcagttcacc agggccggtt attttccgcc agactcgcta cggcatggat ggcaagccga
     8941 tcaaagtgtg gaagttccgt tccatgaaag tgatggagaa cgacaaagtg gtgacccagg
     9001 cgacgcagaa cgatccgcgc gtcaccaaag tggggaactt tctgcgccgc acctcgctgg
     9061 atgaattgcc gcagtttatc aatgtgctga ccggggggat gtcgattgtc ggtccacgtc
     9121 cacacgcggt ggcgcataac gaacagtatc gccagctcat tgaaggctac atgctgcgcc
     9181 ataaggtgaa accgggcatt accggcaggg cgcagattaa cggctggcgc ggcgaaacgg
     9241 acacactgga gaaaatggaa aaacgcgtcg agttcgacct tgagtacatc cgcgaatgga
     9301 gcgtctggtt cgatatcaaa atcgttttcc tgacggtatt caaaggcttc gttaacaaag
     9361 cggcatattg atatgagct
//
LOCUS       DAJTNA010000144         9345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTNA010000144 DAJTNA010000000
VERSION     DAJTNA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.146"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..290
                     /locus_tag="OO161_003087"
     CDS             <1..290
                     /locus_tag="OO161_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoglutarate transporter"
                     /protein_id="HCR5464069.1"
                     /translation="AAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVA
                     NAIFGGSAEYVALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(287..610)
                     /locus_tag="OO161_003088"
     CDS             complement(287..610)
                     /locus_tag="OO161_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5464070.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(656..2011)
                     /gene="pssA"
                     /locus_tag="OO161_003089"
     CDS             complement(656..2011)
                     /gene="pssA"
                     /locus_tag="OO161_003089"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5464071.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(2125..4785)
                     /gene="pat"
                     /locus_tag="OO161_003090"
     CDS             complement(2125..4785)
                     /gene="pat"
                     /locus_tag="OO161_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5464072.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(4817..5515)
                     /gene="tapT"
                     /locus_tag="OO161_003091"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(4817..5515)
                     /gene="tapT"
                     /locus_tag="OO161_003091"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5464073.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(5584..6003)
                     /gene="trxC"
                     /locus_tag="OO161_003092"
     CDS             complement(5584..6003)
                     /gene="trxC"
                     /locus_tag="OO161_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5464074.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            6210..7247
                     /locus_tag="OO161_003093"
     CDS             6210..7247
                     /locus_tag="OO161_003093"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5464075.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(7295..7984)
                     /gene="ung"
                     /locus_tag="OO161_003094"
     CDS             complement(7295..7984)
                     /gene="ung"
                     /locus_tag="OO161_003094"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5464076.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPRQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            8289..8672
                     /gene="grcA"
                     /locus_tag="OO161_003095"
     CDS             8289..8672
                     /gene="grcA"
                     /locus_tag="OO161_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5464077.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(8728..9315)
                     /gene="eamB"
                     /locus_tag="OO161_003096"
     CDS             complement(8728..9315)
                     /gene="eamB"
                     /locus_tag="OO161_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5464078.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2335 a         2399 c         2431 g         2180 t
ORIGIN      
        1 atgccgcttt tggtctggtg atgtgtgccc tgctgatagt gagtttttat acatcaatca
       61 gtggaatact gaaggctgag atgttcccgg cacaggttcg cgcattaggc gttggtctgt
      121 catatgcggt cgctaatgct atatttggtg gttcggcgga gtacgtagcg ttgtcgctga
      181 aatcaatagg aatggaaaca gccttcttct ggtatgtgac cttgatggcc gtggtggcgt
      241 ttctggtttc tttgatgcta catcgcaaag ggaaggggat gcgtctttag tgacgggtca
      301 gttgccagac ggtatagccg gtgcttgcac cggcgacatc ccaggccaaa tccttccagc
      361 tccagccgct cccttcgggg cggctatccc aaagctcttt tgacgccccc aaactgacag
      421 agaacatcaa tccaaacatg gcactgcgat cccggctcat cccctgatgc tgtgaatatt
      481 catttccggc ggcggaaagc atcgccgagg cgataaagtg ttgagcttta tcctgcccgc
      541 tccagctatc gttatccata tggctgcatc cagaaagtaa caatagcgaa cagacaaaaa
      601 gaatacgcaa aacgtggcct ccaaaaaaca aaccccgtac aggacggggt tgtgattaca
      661 ggatgcggct aattaatcgg tcgatgcgga tacggcgcaa acggcggatg agtttacgaa
      721 ccttcaccgg ataatcggca atactttgca gatcgcgata gtgcttaacg atggtggtat
      781 gctcgcggat cagctccagt tctttctctc gctgcggcgc aagctccagt tgtgggtcgt
      841 ggatcaaaat ggcgttttcc agatccagac gccaggcgcg cgggttcagg ttattaccgg
      901 tgatcaacat ccacttatca tcaacccaca tccctttcag gtgataggtg ttgtcgtcat
      961 ctttccataa ccgaaccact agctggtcag tattgacgta atactgcaaa cggctcagga
     1021 aaagacgcag attgatctca tagagataag gcaatgcgcc aattatcttg aaaggttcat
     1081 cttccggaat gtagaagtcg ttcgcggttt tatcaccaac aataatttcg acctttttcc
     1141 cttcgcgcag caactggata atattgcgca caaggattgc tggcaggttg aagtatggcg
     1201 tacagatggt tagtttctgc tcggcacaag gcataagatg gaaaatggtc ttgttcaaca
     1261 gactcgattt ccccagcccc actagcggcg ttacggaaag ctgatcgttg tcggcatcgc
     1321 cctggaaatg ataagcggca tcacgcagct cctggcggaa cagacgaata tcgttcttga
     1381 tttccgggct ttttggccga ttaacatcat ccagacgatt aacgccgcgg ccattcataa
     1441 tattctgtgt aacccattca aacataatgt ctgacatctt acggttacgg ataagatgat
     1501 aacggtcgta gcgatattta tcgtgctgat gcaggtaaac atcgttcagg ctggcaccgc
     1561 tataaagtac gctatcgtcg atgataaagc ctttaaagtg cagaacacca agggcttcac
     1621 gggtattgat tggaacgcca taaaccggaa catctacgcc cgaattttcc tgcgccatgc
     1681 ggcagtacca gtcagcgtta gtgttagatg ccgcagcgcc aatgcgtcca cgttgtgcac
     1741 gatgccagtc gaccagcacc cgcacatcca gttccggacg ctgcctttta gcttcataca
     1801 acgcgttcag aatgcctttg ccaccgtcat cctgttcgag atacagggcg acaatgcaaa
     1861 tgcgctgctt cgcgctggct attttttcca gcagcgtctc ccggaagtcg gcgggagcgt
     1921 aaaagaaatc gacatcatca actgattgag aaatcttggg tagttgggca aggtgttgtt
     1981 gatgtttatt acgcttaaat tttgacaaca tcacagtgca tttcttctct gttcattgaa
     2041 gggtcctctg tgcaatgcag acgacataag cggccaataa taacaccagt cccgattaag
     2101 tggtcaacat ttccagtacc ttactcatga ttcctcgcgt tgggcaagat ttagcgtaag
     2161 cccaacgatc ccctcttcga gctggatatc aacgttaaac ccgagcttgc gggctagcgc
     2221 caccatgcca cgattgtttg gcatcgtaat accattcaga cgttgtagtc cgtgatctcg
     2281 cgtataggta atcaactttt ccattaagcg tcgacctaag cctaaccctt tgagatccga
     2341 gcgaaccagc acagcaaatt cggcatcgat gttatcagga tcggaaatcg cacgcgtgac
     2401 gccgaggatc tcttccgttt gatcaatacg tcgtaccgct acaaacgcca tttcccgatc
     2461 gtagtcgatc tgtgtcatgt tggctaaatc ttcatgggta aattcgttga tctcgctaaa
     2521 gtagcggtaa taaagatctt ctttggtgac tcgcgaaatg aattgctgaa gttgtggctc
     2581 atcttctggc aaaatcgggc ggaacaagca gcgttcaccg tttttcaatt ctacccattc
     2641 ttccagctga tgcggataag ggcgcactgc cagccgactc tcgttatcgc cttcaaacgg
     2701 cgagatatcc agcgtgacat ccagcgcggt aaattcactg ccagaagcca gcaaaggatg
     2761 aatatccaga cgctgaattt ccgggcaatc gacaatcaag ttggaaacct gcaccagaag
     2821 ctggctcaag cctgcaacat ccaatgggcg tagcgcactg cgcgcacgaa tctttttact
     2881 tttgatcccc tgaataacca gatagcgggc caggttcatg ttcagcggcg gcagtgcgac
     2941 gacggcttga tcttcaggac gccactccac accgccttca cccagcatga tcaacggccc
     3001 gaaaaccgga tcgtgcccaa ccacaacccg caactcctga gcgccagcac ggttagccat
     3061 actttgcacc aacaggccgt ggatccgcgc ctgcggccag gccattttta cgcgatcgaa
     3121 aatggcgttt gccgcttgct ggacttcatt ggctgtacgc aagtacagca tgacgccctg
     3181 aacttccgat ttatgtggaa tatccggcga acgcaatttc agcgccaccg gataaccaat
     3241 ctgttcggca atatgcaccg cttcggtgct atcgctggca atccaggtag ggagcgtgtt
     3301 catgccatac gcttgcagga tgggctgaac ttcatgggta tcgagcgacg tagccccttc
     3361 ggcaatcgcc tgttgcaaca gaagatgcgc ttctgcggta ttggaagtca gattgctggg
     3421 caacgccggc gtttcgcgta gttgcttctg attacgccgg tactccacca tatgcataaa
     3481 agcagtgatg gttccttccg gggtacggta ggtcggcagc ccggcttcgc tgaataaacg
     3541 tcgtgcctct tgcgaggagt gctcgccgca ccagttcgtc agcaaagaga catatttgct
     3601 gcggggatga tgctttaccg cttcaattaa tacttgcgcg ctttctgttg cgggagcagc
     3661 ggcgctgggc gaatgaataa ccatcagcgc gtcaaaatcc tggctgtgga gcagaatatc
     3721 cagcgtttta atatagtgct cactgctggc gtcatcgcgt agatcgagcg ggttagatat
     3781 tgccacatgt tctggcagtg catcgcgcag tttctggcag gtttcttcgc ttagcgttgc
     3841 cagcttgcca ttgcgtgacc ataaggcatc cagcgccagc gcggcaggcg cagcaccgtt
     3901 gctgataatc atcagccggt cgccacgtag cgggcgcata tggctaaggg tttccaccgc
     3961 cgaaaacagc tcgtgggtgt cctgtacccg caacaaaccg gcacgctgaa tagccgcatc
     4021 ccatgccggg tccattcctg ccgtggtgtt gagcagtcgc tgtgccgccg ggctacgtcc
     4081 gcttttaatc accagaatcg gtttattacg cgaggcacta cgggccgccg aaacaaagcg
     4141 tcgcgcgtcg cttaactgtt cgagatagag caggatggcg ctggttttac tgtcgcgtgc
     4201 cagatagtca agcaattcat caacgtcgat atccaggctg tcgccgagcg caataaagta
     4261 ggaaaagccc atcttacgct gttgcgccca gtcgaggatg gtgttggaga cggcagccga
     4321 ttgcgaaata aacgccagct tgccgcgttt aatcggcaca ggcgaaaagc tggcattcag
     4381 accttgccag ggagccagta aacccagact gtttggtcca agcaggcgca tgttatggcg
     4441 cagggcgcag gcgcggagat cttcgtgttg cgatgccggg gcggaaagaa taatgcaggt
     4501 tttacagcct ttctcgccga gctcttccag aagagcaaga ttacggctgg cattggtaca
     4561 taaaaccgca aggtcgggtg taaagggcaa gctggcaata tccggccagg ccaacacacc
     4621 caacaccgct ttccaggctg gcgtcaccgg gagtaccggt ccgttaaagc ctcccgccag
     4681 caggttacgc atcatcaggt aacctgcgcg attgggtttc atcgacgcgc caattaccgc
     4741 tatcgatttt ggtcgcagta gtgcttccag tcctcgctga ctcataccgg tctcccgcag
     4801 aagtgaccga atgattttaa acgctttcta actgttctgc tgtgatgcta cccagatgtt
     4861 gcgtttttcc tgccagatag cgtgttttaa agcgggtaaa atgctcgcct aaccctgctg
     4921 ccgccccggt atcgccggcc atatctaaca gtgcgatggc tacctcggca gtacaatatt
     4981 ggccttcagc ctgggcttca cgcaggcgat aggctgaaag ccgggaaaga tcgacggaaa
     5041 tgacgggaag attatccaga tacggacttt tacgaaacat cttgcgagct tccggccagg
     5101 taccatcgag catgataaac agcggtggct taccggcagg tggtgtgaag atcacttccc
     5161 gttgctcatc agcatacgag gcgggaaaga ccaccattgg ctgataatcc gggttttgta
     5221 ccagatccag caaatcctgc gagggttcgg tacgcgacca ttgaaacgca acggtatcag
     5281 gcaaaatatc agcaatcaga cgcccggtat tactgggctt cattggctcg gtgtcgaaca
     5341 tcagcaaaca gaagcgactt tttgcttgtg ctggggtaat tgtcgaacag agacataatt
     5401 tctctggcaa aagacagcgt tggcagcgac gaacgcgatt accgcgggca agaaaaggac
     5461 gtgttgcgcg cgcaatacgc tcggcgcgta actggagaac agcgttttcg gtcataagag
     5521 agcgtcgaaa aaacgccatt gtcgcagagg agaaaacggg gcacaagatg cgccccggta
     5581 agattaaaga gattcgttca gccagctatc gaacggagct ttcggtactg cgccattaag
     5641 catgtcgaca acctgaccgt ttttgaaaat catgatcgtc ggtatactac gaattccaaa
     5701 gcgactgctc aattcacgtt cagcttcggt attcactttc acaaagcgca ctttaccgct
     5761 acgctcttgc gcgacatctt caaaaattgg tgcgaaatta cggcaggggc cgcaccacgg
     5821 tgcccagaag tcgatcacca caggtagatc atccttcagc aatttgtcga gtgtttcacc
     5881 ggtcgcatta atcacctctc cgtcaaacaa gtcgtgaccg cagcgtccgc attttgccgc
     5941 atcttcgatc cgatcgtcgg gaatgcgatt gatggcctga caatgggtac aaacggtatt
     6001 cataactaac ctcgggatga gtaaggagac ttatcggcaa cgacgccggt atgtttctaa
     6061 tatgttacat attatcgggg acattgttcc agacaacaat ctgttttatt caataggtat
     6121 gatagtcgca ggctttggta cgaaatcatg gcgaaccgcg cggacttcgg gtaatctgcg
     6181 cgcttcgcgc agcgctggtg gagaaaagca tgaacgatga aatgaaaggt aaaagcggca
     6241 aggtcaaagt gatgtatgtc cgcagtgatg atgattctga taaacgtacc cacaacccgc
     6301 gtaccgggaa agggggcggg cgtccaggaa aatctcgtgc tgacggtggc cgtcgccccg
     6361 cccgcgatga caaacagagt cagccccgtg accgcaagtg ggaagattcg ccgtggcgca
     6421 cggtttcccg cgcgccgggt gatgagacgc cggaaaaggc cgatcacggt ggcatcagtg
     6481 gtaaaagttt tattgatccg gaagtgttgc gtcgtcagcg tgcggaagaa acccgcgtct
     6541 acggcgaaaa cgcctgtcag gcactgttcc agagccgtcc ggaagcaatt gttcgcgcct
     6601 ggtttatcca gagcgtaacg ccacgtttta aagaagcctt gcgctggatg gcggcaaacc
     6661 gcaaagcgta ccatgtggtg gatgaagctg aattgacaaa agcgtcaggc acggaacatc
     6721 acggcggcgt ttgcttcctg atcaaaaagc gtaacggtac aaccgtgcag cagtgggtaa
     6781 gccaggcagg cgcgcaggat tgcgttctgg cactggaaaa cgaatctaac ccgcataacc
     6841 tgggtggcat gatgcgcagc tgcgcgcact tcggcgtgaa aggtgtcgtg gtgcaggatg
     6901 cggcactgct ggagtcgggg gcggctatcc gtaccgcaga aggcggcgca gagcacgttc
     6961 agccgattac tggcgacaac attgttaacg tgctggatga tttccgtcag gcaggttaca
     7021 ccgtagtgac cacttccagc gagcagggta aaccgctgtt caaaactagt ctgccagcga
     7081 aaatggtact ggtgctgggt caggaatatg aagggttacc ggatgccgca cgcgatccga
     7141 acgatctgcg cgtgaagatt gacggtacgg gcaacgttgc cgggctgaat atctctgtcg
     7201 caaccggcgt tttgcttggt gaatggtggc gtcagaataa agcctgataa atcagccggg
     7261 tggcaactct gccatccggc atttccccgc aaatttactc actctctgcc ggtaatactg
     7321 gcatccagtc aatcggcgtc tcgccacgtt gttccagcca ctgatttgcc agcacaaaat
     7381 ggttgcagcc aaagaatcca cgatgcgccg aaagcggcga cggatgcggt gctttcagta
     7441 catgatggcg ttgcttatct ataatcgccc ctttcttttg cgcatgtgat ccccacaaca
     7501 aaaacaccac gccttcgcgg tgctggttaa tcaggctgat gactttatca gtaaacgtct
     7561 cccaaccgag gctggcgtgg gaatgcgcct gacctgcgcg aaccgtcaac acagtattga
     7621 gcagcagaac gccctgacgc gcccagcttt cgagatagcc atgattaggg cgggtgaagc
     7681 ccggaatagt attttccagc tctttataca tattcaataa tgacggagga atggcaatgc
     7741 cgggacgaac ggaaaatgcc agaccatgcg cctgtctcgg tccgtgataa ggatcctggc
     7801 cgagaatcac cactttaacg tcacccaact cggtaaagcg gaacgcgtta aagacatctt
     7861 tttgtggtgg atagatagtg acgccggact gccgctcgct ggcgacggtc tgaagggtat
     7921 taagaaaata aggttgttgc ttctcttcag ccagcacgtc atgccaggtt aattcgttag
     7981 ccatctcgct ctcctgcgaa tcttcaatcc gcctagctta actgcttctt tctacagaac
     8041 aaaatcatgc tcatttttta gacttaaacg agtgcaaaaa aatgtaaatt ggtaaaattt
     8101 cacacccgca taagttgatg taaaacaata aaatccattt acgaccacaa tcgatgtgtg
     8161 gtttttattg atttaaatca aagaatcaag ggtggttgag gagtatatat acactcaagc
     8221 aacaatggtt ttaccaattg gccgcgacag gctgaacaaa tcaaataatt ttgccgggga
     8281 ggcatcacat gattacaggt atccagatta ctaaagccgc taacgacgat ctgctgaact
     8341 ctttctggct gctggacagc gaaaaaggcg aagcacgttg catcgttgca aaagcaggtt
     8401 atgcagaaga tgaagtggtt gcagtaagca aactgggtga cattgaatac cgtgaagttc
     8461 cagtagaagt gaaaccagaa gttcgcgttg aaggtggtca acacctgaac gttaacgttc
     8521 tgcgtcgtga aactctggaa gatgcagtta agcatccgga aaaatatccg cagctgacca
     8581 tccgtgtatc cggttatgca gttcgcttta actctctgac tccggaacag cagcgcgacg
     8641 ttatcgctcg tacctttact gaaagcctgt aaggcttaag tagcaagtta ctaagcggaa
     8701 gagggcgacc tcttccgctt tttttcgtta atagaaaatg cgtaccgcgc aatagaccag
     8761 caacagggca agcacgatat ttaactggcg accatactgg cgaaacaatc gctgaaacag
     8821 atgccccgcc agcgcccagc acacattgcc aaacgtccca atcatcgcca gcaaaacgct
     8881 gacgccaact acccagctta gcgcctgtgt ttgcggcaga acaaacgtcg acagtgccgt
     8941 aacaccgtac aaaatgattt tgacgttcac aaactgcaaa gcaaagctgg cccaaaagct
     9001 gattggtttt gcctgaagtc cgtcttcctt tgttgggctg gtggcgattt tccacgccag
     9061 ccagacaata tatgccgccc ccgcccaact caaaaggtgt accgctgccg ggtcaatcac
     9121 tgccagtgaa aatgaaatgc ccgcacacag taacatcaca atcaaaaatc ccagactcat
     9181 ccctgccagc acgcgggtac tttgacgaaa tccatgcgac gtagcagagc taagggcgag
     9241 aatattgttc ggtcctggcg tcatagcggt aatcagggtg taagtccaaa aagcacttaa
     9301 aagggtcggt gtcacttgtt atctcctctg ttttggtaga cagta
//
LOCUS       DAJTNA010000145         9301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTNA010000145 DAJTNA010000000
VERSION     DAJTNA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.147"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..290
                     /gene="istB"
                     /locus_tag="OO161_003097"
     CDS             <1..290
                     /gene="istB"
                     /locus_tag="OO161_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5464079.1"
                     /translation="PRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFG
                     QWDQTFAGDAALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            564..746
                     /locus_tag="OO161_003098"
     CDS             564..746
                     /locus_tag="OO161_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5464080.1"
                     /translation="MNHPVIGLVTKADLASMEHISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFSLLHTEDVCR"
     gene            complement(847..1176)
                     /locus_tag="OO161_003099"
     CDS             complement(847..1176)
                     /locus_tag="OO161_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5464081.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1348..2406)
                     /locus_tag="OO161_003100"
     CDS             complement(1348..2406)
                     /locus_tag="OO161_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5464082.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVIFGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(2604..3077)
                     /gene="sbmC"
                     /locus_tag="OO161_003101"
     CDS             complement(2604..3077)
                     /gene="sbmC"
                     /locus_tag="OO161_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCR5464083.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(3195..4361)
                     /gene="dacD"
                     /locus_tag="OO161_003102"
                     /pseudo
     CDS             complement(3195..4361)
                     /gene="dacD"
                     /locus_tag="OO161_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
     gene            4571..5995
                     /gene="sbcB"
                     /locus_tag="OO161_003103"
     CDS             4571..5995
                     /gene="sbcB"
                     /locus_tag="OO161_003103"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5464084.1"
                     /translation="MNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMNPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(6038..6265)
                     /locus_tag="OO161_003104"
     CDS             complement(6038..6265)
                     /locus_tag="OO161_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5464085.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(6279..7337)
                     /gene="yeeE"
                     /locus_tag="OO161_003105"
     CDS             complement(6279..7337)
                     /gene="yeeE"
                     /locus_tag="OO161_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5464086.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWPGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITPPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(7516..8874)
                     /gene="plaP"
                     /locus_tag="OO161_003106"
     CDS             complement(7516..8874)
                     /gene="plaP"
                     /locus_tag="OO161_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5464087.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(8864..8926)
                     /gene="yoeI"
                     /locus_tag="OO161_003107"
     CDS             complement(8864..8926)
                     /gene="yoeI"
                     /locus_tag="OO161_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5464088.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(9141..>9301)
                     /locus_tag="OO161_003108"
     CDS             complement(9141..>9301)
                     /locus_tag="OO161_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5464089.1"
                     /translation="GSITAQTLFEPVWIGWNEQTAGLASGWWRDEILANSAIAGVYAK
                     SDDGKSAI"
BASE COUNT         2556 a         2378 c         2148 g         2219 t
ORIGIN      
        1 ccccccgcct gctcatcatt gatgaaatag gctatctgcc gttcagtcag gaagaagcaa
       61 aactgttctt ccaggtcatc gctaaacgtt acgaaaagag cgcaatgatc ctgacatcca
      121 atctgccgtt cgggcagtgg gatcaaacgt tcgccggtga tgcagcactg acctcagcga
      181 tgctggaccg tatcttacac cactcacatg tcgttcaaat caaaggagaa agctatcgac
      241 tcagacagaa acgaaaggcc ggggttatag ctgaagctaa tcctgagtaa aacggtggat
      301 caatattggg ccgttggtgg agatataagt ggatcacttt tcatccgtcg ttgacacttg
      361 gcaaacaggg gcaaccatgt atgtcagata ttacaatctc acttccgaaa gaagtcaacg
      421 ggtacgttat ctgttcaacc agaatccgac acattctggc tatacgaaag agcatactta
      481 tgcaaatcta tatgcttttc cggcaacaag cagtaatctc agcgctgagg caggaattag
      541 aggtaaaaac gccctaccgg gccatgaatc acccggtcat tggattagta accaaagcag
      601 atttagccag catggaacac atatctttgg tgaaatgttg gttacgggaa gcaggagcgc
      661 acaacgtgtt agtaaccagt gcggttaata acaatggagt tacagaactt ttttccttgc
      721 tgcatacaga agatgtctgt cgctaatttc gataggttct cagatgagaa tgatgacggt
      781 gatacacaga taagaatcaa tgagcaatga gggttgccgg gcaaccctca ttgaataaaa
      841 caggaattac ttcgcttcgc cgtttttcat ttcgcccata gctttcagct ttttggagat
      901 atcgcggcgt tctttggaga gctcggcatt tttgatgatg taatcgtcaa cacgatcttc
      961 atagtcacct ttcatgctgg cgatgatgcc ctggattgct tcaacgctca tccccggctt
     1021 gatgtaatcg ctcaggttgt ccagcagcag gacgcgcttc tggttgtcac ggatcttttt
     1081 ctcaacgtcc tgaatttcac gttgcagttt gttcttacga cggaacagac gaacaaattc
     1141 cagtacgtcc tggaatgaag gcttggtagt ttccatttgt atacccctga taatgtgaga
     1201 gtcggattcg ttttagcaac cgctattcgt tagggccaac attactcata gctgccgcag
     1261 atgacaatgc ttttatccct tttcactatc atacccttta tccttgctga atcgaagcag
     1321 cagcaagatg attctgaagt tcaggaatta tttgcgcaag gcccggcaaa tcagattcga
     1381 tagcaactca agctgatgtg ccgtttccat gttcagccac acataaccat agattggtgt
     1441 ttccactacc ttcaggtcat ggtggttatt gagcaactga cgcagctctt ccacagcatc
     1501 gttcaatttt tccgtattgg caaaaaccgg ctgcggatta ccttcgtaca gcgcatgaac
     1561 aaggctcagc agtatttgct gcatcatgtg ctgggtatca cgaagttttt gcgcgttcaa
     1621 taacacgaaa tggctggggc gcgtggccca gtacgcattg atttgcaact ccagcataca
     1681 aaccagattg cggttaatgg tctggatacc ttcatatatc gattttggaa tacgggtttc
     1741 tttgctggcg ggcgcaatca gcccacgcat tttcacggca tcggtcagga gtttttgtag
     1801 atggctttcc agacgtgggc gttcgagtaa gttcggtgag aatgcagatt gataaacccg
     1861 attatactcg gtcagacttt tcgccagttg aatgcgccag tggatgaacg cccgttgtgg
     1921 ccagataccg gtaaacaaca ttgccagtaa agagccgaag atcacatcgc cgcttcgcca
     1981 taacgccgta tcaatttcac ctgtcgggga acccacaaca attgccagcg tcaccccaat
     2041 caataatcct tgatacggtt tcttgcccag cgccagccaa ccgcaaagaa acatagccgc
     2101 cgcgcaccag actaacatca gcggtaacga aattaactcc agttgcagag cgataagacc
     2161 taaaatcgaa cccaacaccg taccgccaat acgttcgaag gcgcgaggga caacgttacc
     2221 ccagaacgag attggcccca taatcaccac catggtgacc agcggccagg tgctttccgg
     2281 gatagtaaac aggcggataa tgagaaaagt gagcaggaat gccagcgcga cccgagtacc
     2341 atgcacaatg cggtaatggc ggtatacccg gatttcaaac gggcttaatg acttatcggc
     2401 acgcacgcag aaactccgta atgaagatta aaagcgctca taactcatct gtcggccaga
     2461 aggcccgcct ctgaattatc cgacggacaa accgggaaca gtgtaatttc ccggcaggag
     2521 cgaaaaaatt gaaaggcgca tcttactctt ttcgctttca aaaaaagagt ggtcatcgcg
     2581 ttaacacacc gccctgagat gaattagtga tgttttggct gcaccgcaac atacatttcg
     2641 atatcccagt acccatcttc cgcgccattg ttcaaataaa cctcgaagca gggctttggt
     2701 aacatttcat aagcactgtc ctgcaagagg ctattaaaga actgatacca gggtttagca
     2761 aaatcatcac cgactacacg agccaccgca accgcatact gaccacctga aatttctgtc
     2821 agaatgacgc cctcactgtt ttcggggagc gtaaagttat tcggcaccgt cacgacggtg
     2881 tcgcagcgta atttttcggc gggtgtttca tctggattgt cgtaaaagac agcaacccac
     2941 tccttcggca caatattttt gctatctacc cacatcatca actgctcaaa gcctttcttt
     3001 accgtctgtt cccacgggcc aacgagatgg aaacctgcaa cggtacgttt atcttcctgc
     3061 ttaatctcgt agttcatgac gctccatcaa cactgttttt atatacagta taattgcgat
     3121 attaaaacca caaggaatga gtgttgatta tgcgagcaga ctcgcactcc tgccagtctg
     3181 ctgcaaaaga aaggtcaggc cttatggtgg aaataatcac tcaggcgaga aaacatgctg
     3241 ccttccccga cagattccag ggtaaccagc ggccagtgcg ccacctgttt atcacggtcg
     3301 taaagttcaa tttcccctac ccgctgatgg gcgctaattg gcgcggtgag ctctttacca
     3361 tcaagggtat atttggcttt gatatgtgga atttcggctt tcggtagcac catccagaac
     3421 tcttgttccg ttcccaggtc gatattttct ttatcgccat accagatgcg ttccgtaccg
     3481 acctttttcc cacggtgcaa aatttgcacc gtagtaaagt tttgttgccc ccaacgcagt
     3541 aattttcttg cctcttcctc acgacctttt gcactgtcag cccccattac cactgcaatg
     3601 agacgacgct gcccatcgac agccgaagca atgagattaa acccggcacc agaagtatga
     3661 cccgttttca ggccgtcaac attcatggtt ttatcccaca ataacccgtt acggttttgc
     3721 tgggtgatac cgttccaggt gagacttttc tcactgtaca tatgataaaa ctcgggctcg
     3781 ccgtggatga tagcgcgaga aagcacagct aaatcataag ccgagctatg ctgacctggt
     3841 gcatccagac catgcactgt ttcaaaatgc gtatccttga gatgcagctt ctcggcatag
     3901 ttgttcatca tttcaacaaa ctgccgttgc ccaccggcaa tatagtcagc cagtgcaaca
     3961 caagcgtcat ttccggaatc cacaattaaa ccacggctta aatcacgtac cgatacgcga
     4021 tcgccctctt tcaaaaacat cagtgaagaa ccgacaaaca ctggattatc tttcgcccac
     4081 gcatcgcgcc ccacggtgac aatatcgtct ggcgtaatgc gatgactatc gatagtgcga
     4141 tccacgacat agcccgtcat cagctttgtc aggctggcgg gattgcgctg ttgatgctca
     4201 ttacccgcgg tgaggatctg accggtggtg taatccatca gtacctagga gccggcatga
     4261 atctctggag gctgaggtga aaaaggaatg ttttccgccg caaaaccaga cgataagtta
     4321 aaaatgaaca aagaagcagc aataataaga cggcgtttca acagcaaacc ctcaggagtt
     4381 tcaaatagct gttcttttta cggaaatact tatgaactgg ctggaataaa gtgcaagaaa
     4441 atgtgactac cctctcattt ttatctgaca tgatctgttg tcactcgctg tcaaattgtc
     4501 gcgctaaagc tgattagcac ggtgatattt gatactctgg cagacagcag aaataacgga
     4561 tttaacctaa atgaatgacg gtaagcaaca atctaccttt ttgtttcacg attacgaaac
     4621 ctttggcacg caccccgcgt tagatcgccc tgcacagttc gcagccattc gcaccgatga
     4681 cgaattcaat gtcatcggcg aacccgaagt cttttactgc aagcccgcgg atgactattt
     4741 accccagcct ggagccgtat taattaccgg tattaccccg caggaagcac gggcgaaagg
     4801 agaaaacgaa gccgcgtttg ccgcccgtat tcactcgctt tttaccgtac cgaagacctg
     4861 tattctgggc tacaacaatg tgcgtttcga cgacgaagtc acacgcaacg ttttttatcg
     4921 taatttctac gatccttacg cctggagctg gcagcatgat aactcgcgct gggatttact
     4981 ggatgttatg cgtgcctgtt atgccctgcg cccggaagga ataaattggc ctgaaaatga
     5041 tgacggtcta ccaagctttc gccttgagca tttaaccaaa gcgaatggta ttgaacatag
     5101 caacgcccac gatgcgatgg ctgatgtgta cgccactatt gcgatggcaa aactggtaaa
     5161 aacgcgtcag ccacgcctgt ttgattatct ctttacccat cgtaataaac acaaactaat
     5221 ggcgttgatt gatgttcctc agatgaaccc cctggttcac gtttccggaa tgtttggggc
     5281 atggcgcggc aatacaagct gggtggcacc gctggcgtgg catcctgaaa atcgcaatgc
     5341 cgtaattatg gtggatttgg caggagacat ttcgccatta ctggagctgg atagcgacac
     5401 attgcgcgag cgtttatata ccgcaaaagc cgatcttggc gataacgccg ccgttccggt
     5461 taagctggta catatcaata aatgtccggt actggcccag gcgaatacgc tacgcccgga
     5521 agatgccgac cgactgggaa ttaatcgtca gcattgcctc gataacctaa aaattctgcg
     5581 tgaaaatccg caagtgcgtg aaaaagtggt agcgatattc gtggaagccg aaccgtttac
     5641 accttcagat aatgtggatg cacagcttta taacggcttt ttcagtgacg ccgatcgtgc
     5701 agcaatgaaa attgtgctgg aaaccgagcc gcgtaattta ccggcactgg atatcacttt
     5761 tgttgataaa cgaattgaaa agctgttgtt caattatcgg gcgcgcaact tcccggggac
     5821 gctggattat gccgagcagc aacgctggct ggagcaccgc cgccaggtct tcacgccaga
     5881 gtttttacaa ggctatgcag aagagattca gatgctggcg cagcagtatg ccgacgataa
     5941 agaaaaagtg gcgctgttaa aagcgctttg gcagtacgcg gaagagattg tctaatgtga
     6001 agcctccgcc gctggtacgg cggaggacaa ttttgagtca ggctttttga acggtgatgc
     6061 tccacgctgc atcgccaatc tgctgataat cggtgatggc atgtccttct tctgccgccc
     6121 actgtgggat tgcttcagtt gcctgggtgc aatcaaactc aattaccaac tcatcgccgc
     6181 tggccatttc tgccagtgct gcttttgctt ctatgagtgg aaacggacag acctgcgtca
     6241 ccacatccag ctttttaatc gccatcttta caccttactt aatttgccgc agcagttgcc
     6301 agtcgtgcct tacgctgcgg tcgaacatac acaagccagg acgcagtcca gactccgaga
     6361 atcataaata caaggccaat ccagccctgc caggtcatca ttgcagtcat gaccagtcca
     6421 ttaccgatag agcaaccacc tgcaatgctg gcaccgaagc ccatcagtac accacctaac
     6481 ccgctacgta atgttgtttg tgcatcagca gcgcgaacgc ggaactcacg gctcgctttg
     6541 gctgcaataa atgaccccac gaagatccct aacaccagga aaacgcccca gttaatgtat
     6601 ttcacgtcac ccgcgaccag aaattgcaga atattggccg ttgggggagt gattcctaac
     6661 ccaaacatgc gtccggttgc ttcactcagg ggccaggcta aaagcgcaat caaaccgatg
     6721 agtacagcag tgacaaaggg atgccagcgt ttttcaaaca gaatatgagc gatcccggtt
     6781 cggcgcggcg gtaaggtcgc gactttgagt tttggcttct tcaactcttt catcaccacc
     6841 cagagcgtta tcaccagcaa aacggcaacc aacggccaca cagacaaatt aaaagtatca
     6901 gcaatagagt tatgttcagt agtgtagtgc tgcaaggttt gatttaaacc actggcgtgt
     6961 ggagaacgca tcaccgcact catcaccata taagtgaaaa gcgcgatcca actgccgatc
     7021 aatccctcac ccgcgcgata ccaggtcccg gtggcacatc cacccgccag aacaattccc
     7081 agcccgaaaa gatacccacc tataacagta ccaggccacg ggaacgcccc ggcttcgtaa
     7141 gtcagtaggc ccgcctgaat taacgcaaaa acccccacgc tttgtaccga aattgcaatc
     7201 agcaaggcgt aaaacatgcg attatttttc gcgatataca tatcgcgaaa accacctgtc
     7261 aggcaaaaac gcccacgctg catgacaaat cccagcagag caccacaaat tagcccgctt
     7321 aatatcattg aaaacataaa ttaattaacc agatgaatgt taatggggaa attattcatg
     7381 actggtggaa tgcagaccaa taaccaaatt ctctaaatta gaacaaatgg ttattaatga
     7441 ggatttcgat aaaaaagaat aaaaaaaccg gagccaaatg ttcattcgac tccggtttat
     7501 tattagaaaa gatgattatg ctacgtcttc gtactgcgga accggattac ggaaactctt
     7561 ggttacgcaa gccaggtaaa tcagaccgat agcagcccag atcagaccca gaaccattga
     7621 gctttcttcc aggttaaccc acagcgcacc aacggtcagc gcaccacaca tcggcaggaa
     7681 cagatactgg aagtgatctt tcagcgtctt gttacgcttc tcacggatcc agaactgcga
     7741 aataaccgac aggttaacga aggtgaacgc caccagcgca ccaaagttaa tcagcgccgt
     7801 agccattacc aggtcgaagt tgattgccag caaggcaatc gcgccaacca ggatgatgtt
     7861 cattgccgga gtacgccatt tcgggtgtac ataaccgaag aagcttttag ggaacacacc
     7921 gtcacgcccc attacgtaca tcagacgcgc tacgcctgca tgcgccgcca taccggacgc
     7981 cagtacggta atggtggaga agatcagcgc gccaacctgg aacgctttac ccgcaacgta
     8041 cagcatgatt tcaggctgtg acgcatccgg atctttaaag cgagagatat ccgggaagta
     8101 cagctgcagg aagtaagttg caaagatgaa gatcatgccg ccaatcagcg cggtcaggaa
     8161 aatcgcacgc gggatcacgc gctctgcatc tttggtttct tccgacaggt tgctgatgcc
     8221 gtcaaagccg gtaaaggaga agcacaggat tgtcgccccg gtaatcatcg ggataacatg
     8281 cgcatcgcca gaccagaacg gacgagtgct cgccagcgta cccgcgcctt caccttcaaa
     8341 tacgccataa acaaccatgc ccagaatcac cgcgatcagc actacctgca acacgacgat
     8401 tacagtgttg aagttcgcta cggatttcag actacgcagg ttaaaggcgg tcataaaggc
     8461 caccagcgcc accacaaaca tccacgatgg gatggaaggc accagagctt caaaatagat
     8521 tttcgccagc agaatgttga tcatcggcgc gaacagatag tcgagcagag aagaccaacc
     8581 caccataaag ccgacagtcg ggctaatgga tttctgggcg taagtgtatg cagagccagc
     8641 agaaggatag cggcgaacca gcttcccgta gctcagagcc gtaaacagga tcgcaattaa
     8701 tgcgaacgca taggctgtcg gcacatgacc atccgtgagg cctgaaacga taccaaatgt
     8761 atcaaacagc gtcatcggct gcatataggc aagacccatc attacaaccg gaactaacgt
     8821 aagcgtttta cgcaattcca cgcgagaggt gtttggagta acgttatgcg acatggtcat
     8881 tctcctttac ggtgataacc gtcgcgtaag caaaaaattg ccccattttt ttggattcct
     8941 cagcgacaac aactgtcgat ttttagtaaa tatctatccg gtacgaagcc cggcctcttg
     9001 gtatgaatga ttggtttgaa gcaaaaaata accgacgcta attaaacgtc ggtttttagt
     9061 cattttttga cagcggcgca ttgtgcctaa atggggggga aatgacaaga gaatgagagg
     9121 cttgtcataa taatttttct ttaaatggct gattttccgt catcagattt tgcataaaca
     9181 ccggcgatcg cactatttgc taaaatttcg tcccgccacc aaccactggc aagccccgcg
     9241 gtctgttcat tccagccaat ccagacgggt tcaaagaggg tttgggcggt aatgctgcca
     9301 a
//
LOCUS       DAJTNA010000146         9215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTNA010000146 DAJTNA010000000
VERSION     DAJTNA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.148"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..303
                     /locus_tag="OO161_003109"
     CDS             <1..303
                     /locus_tag="OO161_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5464090.1"
                     /translation="FVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLY
                     KLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            355..1511
                     /locus_tag="OO161_003110"
                     /pseudo
     CDS             355..1511
                     /locus_tag="OO161_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(1477..1599)
                     /locus_tag="OO161_003111"
     CDS             complement(1477..1599)
                     /locus_tag="OO161_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464091.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            1802..3487
                     /locus_tag="OO161_003112"
     CDS             1802..3487
                     /locus_tag="OO161_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR5464092.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            3525..5897
                     /locus_tag="OO161_003113"
     CDS             3525..5897
                     /locus_tag="OO161_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5464093.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVINTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKIR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            5954..8737
                     /locus_tag="OO161_003114"
     CDS             5954..8737
                     /locus_tag="OO161_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5464094.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMLDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQERLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         2720 a         2003 c         2006 g         2486 t
ORIGIN      
        1 tttgttcgcc atcagtcaga cgattacccg cataacccca acactgagga cttaagccaa
       61 ttctcttata cggtgagaaa taacgattat tcgcttgtct atacagtaga aaacaatcag
      121 ttaggtctct acaaactgac ggatctacag caaaaagata accttgccgc cgccaatccg
      181 caggtcgtta aagagatgca aggcgtggta agagagttta tcgacagcag ccagccaccg
      241 cttagcgagg taaatcagga gaagtttaac aatatcaaga aagcactaag cgaagcgaaa
      301 taactaaacc ttcatgcggc ggaatttttc gccgccttat tgagcgagat agcgatgcac
      361 gttacagcca agccctccag ttttcaatgt aatctcaaat gtgattactg tttttacctt
      421 gaaaaagagt cgcagtttac tcatgaaaaa tggatggatg acagcactct gaaagagttc
      481 atcaaacaat atatcgcagc gtctggcaat caggtctatt ttacctggca aggcggtgaa
      541 tccactctgg ctggcctgga ttttttccgt aaagttattc actatcaaca acgctatgca
      601 ggccaaaaac gtatttttaa tgcattacaa acgaatggca ttttattgaa taatgaatgg
      661 tgttcctttc tcaaagaaca tgaatttctg gttggtatct cgatcgatgg cccccaggag
      721 ttacatgacc gttacagacg cagtaattca ggtaacggta cttttgcaaa agtgatagca
      781 gccatcgagc gtctgaaatc atatcaagtt gagtttaata cgttaccgtc attaataacg
      841 ttaatgtcca ttaccctctt gaggtttatc attttttaaa atctatcggc agtaaacata
      901 tgcaatttat cgaattgctc gaaaccggga cgccgaatat tgatttcagt ggtcatagtg
      961 agaacacatt ccgtatcatt gatttttctg tgcctcccac ggcttatggc aagtttatgt
     1021 caaccatttt tatgcaatgg gttaaaaacg atgtgggtga aattttcatt cgtcagtttg
     1081 aaagctatgt cagccgtttt ttggggaatg ggcatacaag ttgtattttc caggagtcct
     1141 gcaaggataa tctggttgtt gaaagtaatg gagacattta cgaatgcgac cattttgtct
     1201 atccacagta caaaattgga aacattaata aatctgaact caaaacgatg aacagtgtac
     1261 aactgacagc gcaaaaaaaa cggatttcag cgaaatgtca gcaatgtgca tataaaccta
     1321 tctgcaatgg cggttgtcct aagcatcgta ttactaaagt aaacaatgag actgtttctt
     1381 atttttgcga aggttataaa atcctttttt caaccatggt accttatatg aacgccatgg
     1441 ttgagttagc taagaacaga gtaccgcttt accacattat ggatgttgca aaacaaatgg
     1501 agaataatta ataacgctct cttttaactc cattcttagc ctccttctta tgaccttaac
     1561 aaggcacccg acataaattc ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa
     1621 tcctggctta cgtggtatgc gtcagggcat tagcaatacc cttgattatc aatagcataa
     1681 ccataaccac tgaaaattaa aaatttctat atatttcata ttgttaaatg atacttaaat
     1741 aaatttcatc agtaaatcat tgccatagat aatcattgga tggcacaatc aattttcgct
     1801 aatgataacc attcccatta cgttttgtat gttgatagca aagtatctct gtctgctgaa
     1861 gcccttttgg ctgcgtaaga ataataaaac ttcagtgttg ttaatcatta ttatcctcgc
     1921 aatgatcctt ggcgtggtta aaattcaggt ctggttaaac gactggaata atgatttttt
     1981 caatgcgcta agccagaaag aaaccgataa gctttggcag cttgttcttt ggttccctgc
     2041 cctgctgggg atctttgtat taatatccgt aaacaaaaca tggttaatta aactactgac
     2101 tattcgctgg cgcgaatggt taacagatta ctaccttaat cgctggtttg cagataaaaa
     2161 ctactacttc acgcagatct acggcgagca taaaaataca gataaccctg accaacgtat
     2221 cgctgaagac attctcttac tgatcagcaa aacgctcagt ctttcatttg gcttcatcca
     2281 gtcacttagc atgctgatca cctttaccgt tattctttgg caaagcgcgg gtacgctctc
     2341 attcactgtt ggtggaacag aatggaatat ccaggggtat atggtctata ccgtcgtgct
     2401 cattgtgatc ggtggaactt tatttaccca taaggttggt aagcgtattc gtccgcttaa
     2461 tgtggaaaaa caacgtagcg aagcgacatt tcgaactaat cttgtgcagc ataataagca
     2521 ggcagaactg attgcgctaa gcaatgctga gtctctacaa cgccaggagt tgcgcgacaa
     2581 ttttcatacc attaaagaga actggcatcg tttaatgaat cgtcagcggt ggcttgatta
     2641 ctggcaaaat atttattcgc gctcgcttag cgttcttccc tactttctgt tattgccgca
     2701 gtttattagc gggcagatca atctgggcgg actgatgaaa tcgcgccagg catttatgct
     2761 agtatcgaac aatttaagct ggtttattta taaatatgac gaacttgctg aactggctgc
     2821 ggttatcgat cgcttgtatg agttccatca actcactgaa cagcgcccta cgaataagcc
     2881 taaaaattgc caacatgcgg tacaagtggc taatgcgagt attcgtacgc ctgataataa
     2941 gatcatatta gagaacttga actttcatgt ttcgccaggc aaatggctat tactgaaagg
     3001 ctactctggt gcgggaaaaa ccacactgct taaaacatta tcccactgct ggccgtggtt
     3061 taaaggtgat attttttctc ctgctgacag ttggtatgtg tcacaaacac cgttaatcaa
     3121 aaccggctta ctgaaagaga ttatttgtaa agcacttccc ctgcccgtag acgataaatc
     3181 gttgagcgaa gtactgcatc aggttggtct tgggaaattg gctgcgcgta ttcatgacca
     3241 cgatcgctgg ggagatattc tttccagcgg cgaaaaacaa cgtatcgccc tggcacgatt
     3301 aattttacga cgtccgaaat ggatatttct tgacgaaact acctctcatc ttgaggaaca
     3361 agaggctatc cgcttactgc gtttagtgcg tgaaaaacta cccacaagcg gcgtcattat
     3421 ggttacacat caacccggtg tctggaacct ggccgatgat atttgtgaca ttagcgcggt
     3481 tttataaaaa caataaaagc cctgctgtac ggagatataa ataaatgaag cgagttctta
     3541 ttcctggcgt cattttatgt ggcgctgatg tggcgcaggc cgtcgatgac aaaaacatgt
     3601 acatgcattt ttttgaagag atgacggtct atgctcctgt ccctgtaccc gtaaacggca
     3661 acacgcatta caccagtgaa agcatcgagc gtttaccgac cgggaatggc aatatcagcg
     3721 atctgctgag aaccaaccct gcggtacgca tggattcaac gcaaagtacc tcgttgaacc
     3781 agggagatat tcgccctgag aaaatctcta ttcacggtgc gtcgccctac cagaatgcct
     3841 atttgattga cggtattagt gcaactaata acctgaaccc agcgaatgag tccgatgcca
     3901 gtagtgcaac caatattagc gggatgtcac aggggtatta tcttgatgtc agcttactgg
     3961 acaatgtgac gctttatgac agttttgtgc cggttgaatt tggtcgcttc aatggcgggg
     4021 taattgatgc aaagatcaaa cgcttcaacg ctgatgatag caaggtgaaa ttgggttatc
     4081 gcactacgcg ttcggactgg ttaacatcgc atatcgatga gaataacaag agcgcattta
     4141 atcaaggttc ttcaggaagt acttatttct ctccagattt taaaaagaac ttttatacct
     4201 tgtcgtttaa tcaggagctg gctgataact ttggcgttac cgccggttta tcgcgccgcc
     4261 agtctgatat cacccgcgcg gattatgttt cgaatgacgg cattgtcgcc ggtcgggcac
     4321 agtataaaaa cgttatcaat actgcattga gcaaatttac ctggtttgcc agcgaccgct
     4381 ttacccacga tttaacctta aaatataccg gctccagccg tgattataat accagtacct
     4441 tcccgcagtc tgatcgcgaa atgggtaata aatcctatgg tcttgcatgg gatatggata
     4501 cgcaactcgc atgggccaaa atacgtacca ccgttggttg ggatcatatt agtgattata
     4561 cccgtcacga tcatgacatc tggtacaccg aactttcatg tacatatggt gatattacag
     4621 ggcgttgtac ccgtggcgga ttaggacaca tttcccaggc tgtagataat tacaccttca
     4681 aaacacgcct ggactggcaa aaattcgccg tgggtgatgt ttcgcatcaa ccctactccg
     4741 gcgcggaata catctattcc gatgcatgga ctgaacgcca taaccagtct gaatcctatg
     4801 tgattaatgc tgcaggaaag aaaactaacc ataccattta ccataaaggt aaaggcagcc
     4861 taggaattga caactacacg ctgtatatgg cggatcacat tagctggcgg aatgtgtcgt
     4921 taatgcccgg tgtgcgttat gactatgaca actatctgtc aaaccacaat atctccccgc
     4981 gctttatgac ggaatgggat atttttgctg atcaaacctc aatgattacc gccggttata
     5041 accgttacta tggcgggaat attcttgata tgggattacg tgatatccgc aatagctgga
     5101 cggaatcggt atcaggtaat aaaaccctga cgcgctatca gaatttgaaa acgccttata
     5161 acgatgaact ggcaatggga ttgcagcaaa aaatcggtaa gaacgttatt gcacgcgcaa
     5221 actatgttta ccgtgaagcg catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga
     5281 ctaaaaccac cattactgaa tataacaacg atggcaaaac caaaacgcat tcgtttaacc
     5341 tcagttttga actggccgaa cccctgcata tccgccaggt agatattaac ccgcaaattg
     5401 tctttagcta tatcaagagc aagggcaact tgtcgttaaa caatggttat gaggagagca
     5461 ataccggtga taaccaggtg gtttataacg gtaatctggt ctcttacgat agcgttccag
     5521 tggcagattt taataaccca ttaaagatct ccttaaacat ggatttcacg catcaaccga
     5581 gcgggttagt gtgggcgaat acgctggcct ggcaagaagc gcgtaaagct cgcattatcc
     5641 ttggtaagac aaatgcgcaa tacatcagcg aatattcaga ttacaagcag tatgttgacg
     5701 aaaaactgga tagcagcctg acctgggaca cccgcttgtc ctggacgcca caatttctga
     5761 aacaacaaaa cctgacgatc agtgccgata ttctcaatgt actggatagc aaaaccgctg
     5821 ttgatacaac gaataccggt gtggcgacct acgccagtgg ccgtactttc tggcttgatg
     5881 tcagcatgaa attttaatag ttcggatttt acttaaccgg gtaaattcgc ccggttttcg
     5941 catggagata attatgagaa acctctgttt cttactgacg ttagtggcaa ctctgttgct
     6001 ccccgggcgg ctgattgccg ccgccttacc gcaggatgaa aagttaatta ccgggcaact
     6061 ggacaatggc ttgcgatata tgatttatcc gcatgctcaa ccaaaggatc aggtaaattt
     6121 atggctgcaa attcataccg gttcattgca ggaagaagac aatgagcgcg gcgtggctca
     6181 ttttgtagaa catatgatgt ttaacggcac aaaaacatgg ccgggtaata aagtcatcga
     6241 aacatttgag tcaatgggcc tgcgttttgg tcgcgatgtt aatgcctata ccagctatga
     6301 cgaaacggtg tatcaggtga gtttgccgac tacgcagaaa caaaatctgc aacaagtgat
     6361 ggcaatcttc agtgaatgga gtaatgccgc aacctttgaa aaactcgaag tagacgctga
     6421 acgtggcgta attactgagg aatggcgtgc ccatcaggat gcgaaatggc gcacctctca
     6481 ggcgcgccgc cctttcctgc tggctaatac ccgtaattta gaccgtgaac ctatcggcct
     6541 gatggatact gtcgccacgg tcacaccggc acaattgcgc caattttatc aacgctggta
     6601 tcaaccaaat aatatgacct ttatcgtggt cggcgatatc gacagtaaag aagcgctggc
     6661 gctgataaag gataatttaa gtaagcttcc ggctaacaaa gcagctgaaa atcgcgtctg
     6721 gccgacaaaa gccgaaaacc acctgcgctt taatatcatt aatgataaag aaaaccgggt
     6781 gaacggcatc gcactctatt atcgcctgcc aatggtacaa gtgaacgatg agcaaagctt
     6841 tatcgaacaa gctgaatgga gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg
     6901 catacagtcg ggcgagttga agactatttc tggcggcact gcgcgcagcg ttaaaattgc
     6961 acccgattat cagtcgctgt ttttccgtgt aaatgcacga gacgataata tgctggatgc
     7021 tgcgaatgca ttaatggcag agttggcaac cattgatcag catggttttt ctgctgaaga
     7081 actcgatgat gtcaaatcta cccgcctcac ctggctgaaa aatgcggttg atcagcaagc
     7141 tgaacgtgat ttacgtatgc tgaccagtcg cctggcatcc agctcattaa ataatacgcc
     7201 gttcttgtcg ccggaagaga catatcaact ttcgaaacgt ctgtggcagc aaattaccgt
     7261 gcaaagtctg gcggaaaaat ggcagcagtt aagaaagaac caggacgcat tttgggagca
     7321 aatggtaaac aatgagcttg ccgccaaaaa agcattgtct cctgcggcta tcctggcgct
     7381 ggaaaaagag tacgccaaca aaaagctggc ggcttacatc ttcccaggca gaaatttatc
     7441 gttaacagta gacgctgacc cacaggcgga aattagcagc aaagaaacgc tggctgagaa
     7501 tctgacatca ttaacacttt ccaatggtgc cagggttatt ctggcaaaat ccgcgggtga
     7561 agagcaaaag ctacaaatta ctgccgtatc gaataaaggc gatttaagtt tccctgcgca
     7621 gcaaaaatca cttatcgcgc tggcaaataa agcagtaagc ggaagcggcg ttggtgagct
     7681 ctcctcttcc agcctgaaac gctggagtgc ggaaaattcg gtaaccatga gcagtaaagt
     7741 cagtggcatg aatacgttgc tctccgttag cgcgcggact aataaccctg aacctggttt
     7801 tcagttgatt aaccagcgaa tcacccacag cacgattaac gacaatattt gggcatcgct
     7861 acaaaatgct caaattcagg cattgaaaac gctcgaccag cgtccagcgg agaaattcgc
     7921 ccagcagatg tatgagacgc gctatgctga tgaccgcacg aaattactgc aagaaaatca
     7981 gattgtacag tttactgccg cagatgcgct ggctgccgat cgccagttgt tttcatctcc
     8041 agcggatatc acgtttgtca ttgtcggtaa tgtctcagaa gacaaactcg tggcgttaat
     8101 tacgcgttac ttaggatcaa tcaaacactc tgattcgcca ttagccgcag gtaaaccatt
     8161 aactcgcgcg acggacaacg catcggttac tgtaaaagaa caaaatgaac ctgtggcaca
     8221 ggtttcacag tggaagcgtt atgattcccg gacacctgtt aatctggcga cgcgtatggc
     8281 gctcgatgct tttaacgtcg cactggcaaa agatctacgt gttaatattc gtgaacaggc
     8341 atctggagca tacagcgttt cttctcgcct ctcggttgat cctcaggcca aagatatcag
     8401 tcatttgctg gcttttactt gtcaaccaga acgacatgat gaactgttaa cgttagcgaa
     8461 tgaagtgatg gttaagcgtc tggctaaagg gatcagtgag caagaactga atgaatatca
     8521 gcaaaacgtt cagcgcagcc tcgatatcca acagcgtagc gttcaacaat tagcgaacac
     8581 tattgtaaat agtcttattc aatatgacga tcctgcagca tggactgagc aggagcgatt
     8641 gttgaaacaa atgacggtag agaatgttaa cactgccgtt aaacaatatc tttctcatcc
     8701 ggtaaatact tataccggag tattattgcc aaaataataa cagccccgtc aacacatcgt
     8761 tggcggggat tttagcaata ttcgctattt ttatgtaata attttataaa tgcgttcaaa
     8821 ataataatca agtactaata gtgatatttt taagtctgat ttttacgtga taattcagga
     8881 gacacagaat gcgcataaaa ataacagcat aaaacacctt accaccaccc aagaatttca
     8941 tattgtattg tttttcaatg aaaaaaacat tattcgcgta atatctcacg ataaataaca
     9001 ttaggatttt gttatttaaa cacgagtcct ttgcacttgc ttactttatc gataaatcct
     9061 acttttttaa tgcgatccaa tcattttaag gagtttaaaa tggataagaa gcaagtaacg
     9121 gatttaaggt cggaactact cgattcacgt tttggtgcga agtctatttc caccatcgca
     9181 gaatcaaaac gttttccgct gcacgaaatg cgcga
//
LOCUS       DAJTNA010000147         9131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTNA010000147 DAJTNA010000000
VERSION     DAJTNA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.149"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            141..2010
                     /gene="kup"
                     /locus_tag="OO161_003115"
                     /pseudo
     CDS             141..2010
                     /gene="kup"
                     /locus_tag="OO161_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="OO161_003116"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="OO161_003116"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5464095.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..2972
                     /locus_tag="OO161_003117"
     CDS             2604..2972
                     /locus_tag="OO161_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein"
                     /protein_id="HCR5464096.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGNT
                     RGVDVGAKKEIYQLINQFNADGLSIILVSSEMPEVLGMSDRIIVMHEGHLSGEFTREQ
                     ATQEVLMAAAVGKLNRVNQE"
     gene            2977..3942
                     /gene="rbsC"
                     /locus_tag="OO161_003118"
     CDS             2977..3942
                     /gene="rbsC"
                     /locus_tag="OO161_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5464097.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQNSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            3967..4853
                     /gene="rbsB"
                     /locus_tag="OO161_003119"
                     /pseudo
     CDS             3967..4853
                     /gene="rbsB"
                     /locus_tag="OO161_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            4979..5908
                     /gene="rbsK"
                     /locus_tag="OO161_003120"
     CDS             4979..5908
                     /gene="rbsK"
                     /locus_tag="OO161_003120"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5464098.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            5912..6904
                     /gene="rbsR"
                     /locus_tag="OO161_003121"
     CDS             5912..6904
                     /gene="rbsR"
                     /locus_tag="OO161_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5464099.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(6870..8297)
                     /gene="mdtD"
                     /locus_tag="OO161_003122"
     CDS             complement(6870..8297)
                     /gene="mdtD"
                     /locus_tag="OO161_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5464100.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSMGIVGNIATRL
                     GTGSVQFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(8320..9012)
                     /locus_tag="OO161_003123"
     CDS             complement(8320..9012)
                     /locus_tag="OO161_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5464101.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2218 a         2203 c         2413 g         2297 t
ORIGIN      
        1 aaggggggta tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggctat tggagttgtc tacggcgata ttggtaccag cccgttatat acacattcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaaaca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc aatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg ggggcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggt tggttgccat tgagcctcgg tacggtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgtaccgaa
     1681 gacgcgccat atgtccataa tgtccgtcgg gtacagattg aacaactgtc gctcactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gcgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggagtcgtt acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta atacccgtgg cgtagatgtc ggcgcgaaaa
     2761 aagagatcta tcaactgatt aaccagttca acgccgatgg cttgagcatc attctggtgt
     2821 catcggagat gccagaagta ttaggcatga gcgatcgcat catcgtcatg catgaagggc
     2881 atctcagcgg ggaatttact cgtgagcagg ccacccagga agtgttaatg gctgccgctg
     2941 tgggcaagct taatcgcgtg aatcaggagt aaaaaaatga caacccagac tgtctctggt
     3001 cgccgttatt tcacgaaagc gtggctgatg gagcagaaat cgcttatcgc tctgctggtg
     3061 ctgatcgcga ttgtctcgac gttaagcccg aactttttca ccatcaataa cttattcaat
     3121 attctccagc aaaactcggt gaacgccatt atggcggtcg ggatgacgct ggtgatcctg
     3181 acgtcgggca tcgacttgtc tgtaggttct ctgttggcgc taaccggcgc agttgctgca
     3241 tctatcgtcg gcattgaagt caatgcgctg gtggctgtcg ctgctgctct cgcgttaggt
     3301 gccgcaattg gtgcggtaac cggggtgatt gtagcgaaag gtcgcgttca ggcgtttatc
     3361 gctacgctgg ttatgatgct tttactgcgc ggcgtgacca tggtttatac caacggtagc
     3421 ccagtgaata ccggctttac tgagaacgcc gatctgtttg gctggtttgg tattggtcgt
     3481 ccgctgggcg taccgacgcc agtctggatc atggggattg tcttcctcgc ggcctggtac
     3541 atgctgcatc acacgcgtct ggggcgttac atctacgcgc tgggcggcaa cgaagcggca
     3601 acgcgtcttt ctggtatcaa cgtcaataaa atcaaaatca tcgtctattc tctttgtggt
     3661 ctgctggcat cgctggccgg gaccattgaa gtggcgcgtc tctcctccgc acaacccacg
     3721 gcggggactg gctatgagct ggatgctatt gctgcggtgg ttctgggcgg tacgagtctg
     3781 gcgggcggaa aaggtcgcat tgttgggacg ttgatcggcg cattaattct tggcttcctt
     3841 aataatggat tgaatttgtt aggtgtttcc tcctattacc agatgatcgt caaagcggtg
     3901 gtgattttgc tggcggtgct ggtagacaac aaaaagcagt aataacgact acaggacatc
     3961 ttgaatatga acatgaaaaa actggctacc ctggtttccg ctgttgcgct aagcgccacc
     4021 gtcagtgcga atgcgatggc aaaagacacc atcgcgctgg tggtctccac gcttaacaac
     4081 ccgttctttg tatcgctgaa agatggcgcg cagaaagagg cggataagct tggctataac
     4141 ctggtggtgc tggactccca gaacaacccg gcgaaagagc tggcgaacgt gcaggactta
     4201 accgttcgcg gcaccaaaat tctgctgatt aacccgaccg actccgacgc agtaggtaat
     4261 gctgtgaaga tggctaacca ggcgaacatc ccggttatca ctcttgaccg ccaggcaacg
     4321 aaaggtgaag tggtgagcca cattgcttct gataacgtac tgggtggcaa aatcgccggt
     4381 gattacatcg cgaagaaagc gggtgaaggt gccaaagtta tcgagctgca aggcattgct
     4441 ggtacatccg cagcccgtga acgtggcgaa ggcttccagc aggccgttgc tgctcacaag
     4501 tttaatgttc ttgccagcca gcagattttg atcgcactaa aggtttgaac gtaatgcaga
     4561 acctgttgac cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc
     4621 tgggcgcgct gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg
     4681 acggtacacc ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc
     4741 agctacccga tcagattggc gcgaaaggcg tcgaaaccgc agataaagtg ctgaaaggcg
     4801 agaaagttca ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat
     4861 caggttgtat gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa
     4921 agaaaagcag ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat
     4981 gcaaaacgca ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct
     5041 tcaatctttt cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg
     5101 cggcaaaggc gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat
     5161 tgcctgtacg ggtgatgaca gcattggtga gagcgttcgc cagcagctcg ccactgataa
     5221 cattgatatt actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt
     5281 tgttaatggc gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgccctttc
     5341 cccggcgctg gtggaagcgc aacgtgagcg tattgccaac gcgtcagcat tattaatgca
     5401 gctggaatca ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac
     5461 tatcgttgcg cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt
     5521 ggacattatt acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa
     5581 tgatgaagat gcagcgaagg cggcgcaggt actgcatgaa aaaggtatcc gtactgtact
     5641 gattacttta ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc
     5701 tggattccgg gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt
     5761 aatcacggcg ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc
     5821 agctgcgatt gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat
     5881 cgacgcattt ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgcct
     5941 ggcgggcgtt tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga
     6001 agcgattacc gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagccct
     6061 ggcgcgtagc ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac
     6121 caatcctttc tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta
     6181 tagtctcgtc ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac
     6241 gctgatgcaa aaacgcgttg atggcttgct gttactgtgc accgaaacgc atcaaccttc
     6301 gcgtgaaatc atgcaacgtt atccgacagt gcctactgtg atgatggact gggctccgtt
     6361 cgatggcgac agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca
     6421 atatctgatc gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac
     6481 tccggcgcgc ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat
     6541 tcctgatggc tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg
     6601 ccaactgtta tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc
     6661 tgttggcgtt taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt
     6721 gattggctat gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca
     6781 ccaaccgaaa gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca
     6841 gccgaccctt cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc
     6901 ttagatttac gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac
     6961 attgctgctg aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg
     7021 acagtcgttg tgccttccat cccttcataa acgcgaagga cggccgcact tacagcaacg
     7081 cctaaactaa tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg
     7141 tcatcggtca gatcggcaag tgtgatggta ttcatcgcgg taaattgcgt cgacatagcc
     7201 atccctaata taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa
     7261 gagaactgag cgatcattag cccaataatc accgtgatcc ccactaacgt atggcgatag
     7321 cccagacgac gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc
     7381 ggtgccatca tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat
     7441 ggcataagga actgtacact accggtcccc agacgggttg caatattgcc tacgataccc
     7501 atcgagaaag tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtgtgtcgc
     7561 gcatggagaa tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc
     7621 cagctggcga caatcttttc cccgaatagc tctattccgc ttgagaagag aacaaggctg
     7681 aggccaaaca gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc
     7741 atatgtttgc gcgcgtaaag aaggcccgca atacctatgg ggatattgat taaaaatatc
     7801 cagtgccagg ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc
     7861 accagacccg gcatggcgac aaaattcaat acaggaagaa gttcattacg aggataagca
     7921 cgcagtaagg ccagccgagc aacaggcatc atcattgcgc cgcctatccc ctgaataacc
     7981 cggaagacaa ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg
     8041 aacagactca cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg
     8101 cttaccggaa taaacatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg
     8161 agaggagaac gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca
     8221 tcaagtgcct gcatgaagaa ggccatcgcc gcgatccacg gcaaacccgc catactacgc
     8281 ttctttttat cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag
     8341 cagcgcctga caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat
     8401 cgcctggtgc agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg
     8461 ataaaccgaa tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata
     8521 gatatgctca tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttcttttt
     8581 taatgccgcc atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac
     8641 cgttgctgcc agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc
     8701 gatgacctga tgaaagttct cttctgtcat ccaccaggta agcaattcct gatcaagaaa
     8761 attccagttt gattgtggca tgacccgggt accaattcgc ggtcgcggta aaaccatccc
     8821 ttttgccgtt aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc
     8881 gcccagctca atctcaccag gcaaaatggt gccgggttca tattcacctt ttaagatgcg
     8941 ttgcgccagc ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc
     9001 gcttaaaggc attactaatc ttcctttttc tttttattcc tccttagtat gccaccagga
     9061 agtgtgatta cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggctt
     9121 ttgctgaaaa a
//
LOCUS       DAJTNA010000148         8977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTNA010000148 DAJTNA010000000
VERSION     DAJTNA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.150"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            68..430
                     /locus_tag="OO161_003124"
     CDS             68..430
                     /locus_tag="OO161_003124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464102.1"
                     /translation="MKFHLDELKRMATLDNFARFLNESSTNEKCLSCGDTDMYMYLTN
                     IVEVGPEPKTAEECDLGTFVMLDYIGPFTGYPGYEGHDRENIHNYEFRLTCNRCGFVH
                     RYSARAFMNWVGKQGDEK"
     gene            538..867
                     /locus_tag="OO161_003125"
     CDS             538..867
                     /locus_tag="OO161_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464103.1"
                     /translation="MNDDLERRVSCLESDVTEIKNNLITLTTRSESFATKSDVLEIRE
                     GLRLEMAESRQSLKSEMADLRQSLKVEMAEHRTELQKSFANQTWLLTGIVLSAMAVLV
                     AVVTVIK"
     gene            1076..2055
                     /gene="btuC"
                     /locus_tag="OO161_003126"
                     /pseudo
     CDS             1076..2055
                     /gene="btuC"
                     /locus_tag="OO161_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
     gene            2117..2668
                     /gene="btuE"
                     /locus_tag="OO161_003127"
     CDS             2117..2668
                     /gene="btuE"
                     /locus_tag="OO161_003127"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5464104.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCATTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            2668..3417
                     /gene="btuD"
                     /locus_tag="OO161_003128"
     CDS             2668..3417
                     /gene="btuD"
                     /locus_tag="OO161_003128"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5464105.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQHVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSRDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            3495..3959
                     /locus_tag="OO161_003129"
     CDS             3495..3959
                     /locus_tag="OO161_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5464106.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            4206..4919
                     /gene="ydiV"
                     /locus_tag="OO161_003130"
     CDS             4206..4919
                     /gene="ydiV"
                     /locus_tag="OO161_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5464107.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            4982..6418
                     /locus_tag="OO161_003131"
     CDS             4982..6418
                     /locus_tag="OO161_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5464108.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAELGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6422..6613)
                     /gene="hemP"
                     /locus_tag="OO161_003132"
     CDS             complement(6422..6613)
                     /gene="hemP"
                     /locus_tag="OO161_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5464109.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(6745..7791)
                     /gene="aroH"
                     /locus_tag="OO161_003133"
     CDS             complement(6745..7791)
                     /gene="aroH"
                     /locus_tag="OO161_003133"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5464110.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(7948..8781)
                     /gene="ppsR"
                     /locus_tag="OO161_003134"
     CDS             complement(7948..8781)
                     /gene="ppsR"
                     /locus_tag="OO161_003134"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5464111.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRTANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         2186 a         2042 c         2382 g         2367 t
ORIGIN      
        1 atgcccatct tgcgccggat tacctgcagg atgcggcgag gtttaatcca ctaggaggtg
       61 cttaagagtg aagtttcatt tagatgaact taaaagaatg gcaacgctcg ataattttgc
      121 gcgttttctc aatgagtcgt ctacgaatga aaaatgtttg tcgtgtggcg atacggatat
      181 gtacatgtat ttgacgaata ttgttgaggt ggggcctgag ccgaaaactg ctgaggagtg
      241 tgatttaggc acttttgtta tgcttgatta catcggtcca tttactgggt atccgggata
      301 tgagggacat gatcgggaaa atatccataa ctatgaattc cgactgacct gtaacagatg
      361 tgggtttgtt catcgttact cggcccgggc ctttatgaac tgggtaggca agcagggtga
      421 tgagaagtga tggcagagaa tgttgcacat atttcgcggt tcagaaaaaa gaaagagaat
      481 gcctataatg tatccaacaa ccaagtaaca ggaagtggta atgatggagg tgatggcatg
      541 aatgacgacc ttgaacggcg ggtcagttgc ctggaaagtg atgtgactga aatcaaaaac
      601 aacctgatta ctctcaccac aagaagcgaa tcctttgcta ccaaatctga tgtactggag
      661 attcgcgaag ggttgaggct tgagatggca gaatcgcgcc aatccctgaa gtctgagatg
      721 gctgatttac gccagtctct gaaagttgag atggctgagc atcgtactga acttcagaaa
      781 tcatttgcaa atcagacttg gttactcaca ggtattgtcc tgtctgcgat ggccgtgctt
      841 gtggctgtgg ttactgttat taagtaaatt tatggcggcg atatgatcca caaagtgacc
      901 acatccctgt tatttgttgt ggttggctgt gtttttgtgt gtctgtaagt ctttgataat
      961 tatctaactt gttgattttt acttgtgttt atggccgctc tgcggccttt tttcttttca
     1021 ctgtcgaaga gtcaccgtaa aatcaacgcc atgacacttc agcagaacgg ataccatgct
     1081 gacacttgcc cgccaacaac agcgacaaaa tattcgctgg ttattatgcc tgtcagtttt
     1141 gatgctgctg gcgcttctct taagcctttg cgccggtgaa caatggatct cgctaggtga
     1201 ctggtttact cctcgtggcg aactgttcgt ctggcaaatt cgcctgccac gtacgctggc
     1261 tgtattgctg gttggtgcgg cgctggctat atccggcgct gtaatgcagg cgttgtttga
     1321 aaatcctctg gcagaacctg gactacttgg cgtctctaac ggcgcaggcg tggggcttat
     1381 cgccgcggta ttgcttgggc aagggcaact ccccaactgg gcgctagggc tgtgtgcgat
     1441 tgctggcgcg cttatcatca ctttaatact cttacgtttc gcccgtcgtc atctttcgac
     1501 cagtcggtta ttgctggctg gcgttgcatt agggattatc tgtagcgcac taatgacgtg
     1561 ggctatctac ttttccacct ctgttgattt acgtcagctg atgtactgga tgatgggcgg
     1621 ttttggcggc gtagactggc ggcaaagctg gctgatgctg gcattgatcc ccgtgttgtt
     1681 gtggatctgt tgtcagtcca ggccgatgaa tatgttagca cttggcgaga tctcggcgcg
     1741 gcaactgggt ttacccctgt ggttctggcg caatgtgctg gtggccgcga ccggctggat
     1801 ggttggcgtc agtgtggcgc tggcgggtgc tatcggcttt attggtctgg tgatcccaca
     1861 tattcttcgg ttgtgtggtt taaccgatca tcgcgtatta cttcccggct gcgcgctggc
     1921 aggggcgagc gcattgctgc tggccgatat tgtagcgcgc ctggcattag ctgcccagag
     1981 ctgcctattg gcgtggtcac cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta
     2041 aaagcaagac gttagccgca aaaagacggt ctatgattaa aagctaagat ttttctgacc
     2101 acacccagga gaaacgatgc aagattccat tctgacgacc gtagtgaaag atatcgacgg
     2161 tgaagtgacc acgctggaga agtacgccgg taatgtgctg ttgattgtca atgtcgcctc
     2221 aaagtgtggc ttaacgccgc aatatgagca gttggagaat attcagaaag cctgggccga
     2281 tcgaggtttt gtggtgctgg gattcccgtg caaccagttt ctggaacaag aaccgggcag
     2341 cgatgaagag attaaaactt actgtgccac cacatggggg gtgacgttcc cgatgttcag
     2401 taagattgaa gttaacggcg aaggacgcca tccgctgtat caaaaattga ttgccgcagc
     2461 gccgaccgca gtcgcgccgg aagagagcgg attctatgcc cgtatggtca gcaaaggtcg
     2521 tgcaccgctg taccctgatg atattttatg gaattttgag aaatttttag ttggcaggga
     2581 cggattggtc atccagcgtt tttccccgga tatgacgccg gaagacccca ttgtgatgga
     2641 aagcattaaa ctggcgttgg caaaataatg tctattgtga tgcagttaca ggatgttgcg
     2701 gaatctaccc gcctggggcc actttctggc gaggttcggg ctggggagat cctgcacctg
     2761 gtggggccga atggcgcggg taagagtacc ttgcttgcac gaatggccgg aatgaccagc
     2821 ggtaagggaa gcattcagtt cgcggggcaa cccctggaag catggtccgc aacaaaactc
     2881 gcgctgcatc gcgcctatct ttcacaacag cagacgccgc cgtttgcaat gccggtctgg
     2941 cactacctga cactgcatca gcacgataaa acgcgtaccg aactactgaa tgatgtcgca
     3001 ggggcgctgg ctcttgatga caaactcggt cgtagcacca atcaactttc cggcggtgaa
     3061 tggcaacacg tacgtcttgc tgcggtggtg ttgcaaatca caccacaagc caatcccgca
     3121 ggccaattgc tgcttcttga tgagccgatg aacagtcgtg atgttgcgca acaaagtgcg
     3181 ttagacaaaa ttctgagcgc gctgtgtcag caaggactgg cgattgtgat gagcagtcac
     3241 gatctcaacc acacgttgcg tcatgcgcat cgggcgtggt tgctaaaagg tggaaaaatg
     3301 ctggccagtg gacgcagaga agaggtgctc acgccgccaa atctggcgca ggcctatggg
     3361 atgaattttc gccgtctgga tatcgaaggt cacagaatgc tgattttgac catctgattt
     3421 cattagtatc ttgaaaatcg ggtaattacg acgctaaatt aatgcaagaa taaaaaacag
     3481 aggaaccgta cggaatgcgt ttctgcctta ttttgatcac agcactgttt ctggccgggt
     3541 gtagccacca taaagcaccg ccgccaaatg ccagactttc tgattcgatt accgttattg
     3601 ccggtttgaa cgaccagcta caaagctggc atggcacgcc gtatcgttat ggtggcatga
     3661 cgcggcgcgg tgtggactgt tcgggatttg tggttgtgac gatgcgcgat cgtttcgatt
     3721 tgcagctgcc ccgtgaaacc aaacaacaag cctctattgg cacgcaaatt gataaagaca
     3781 agttgctgcc aggtgacctg gtctttttca aaacggggtc cggacaaaat ggtttgcatg
     3841 taggtattta cgataccaac aaccaattta tccacgcctc taccagcaag ggagtgatgc
     3901 gttcctcact tgataatgtc tattggcaga aaaatttctg gcaggcgaga cgaatctagt
     3961 atgtcgaaaa atggacggcg atgatacatc gccgtccatt tactcctgaa tacagtagta
     4021 ttttgcagac aaaaattccg taaatggatt attcgtttat aatcaggata atctaaaaaa
     4081 aggacccctg aaaaagtggt taacatggaa caaatgaaag ttactccttc tgtaattgtt
     4141 atgtcttatg gcctcatccc ttttagccgg atactgaaaa acatccttcg agagggacgg
     4201 ttaccatgaa gatttttttg gagaatcttt atcattctga ttgctatttc ctgccgataa
     4261 gagataacca gcaagtcctt gtcggcgttg agttaattac ccatttctcc agtgaagatg
     4321 gcacggtgcg tatcccgacg agtcgggtta tagcgcaact taccgaggag caacattggc
     4381 aattattttc agagcagttg gaattactaa aatcatgcca acattttttt attcaacata
     4441 aactctttgc ctggcttaat ttaacgccac aagtggctac attattatta gagagggata
     4501 attacgcagg cgaactatta aaatatccgt ttattgaact gttgattaat gagaattacc
     4561 cgcatctcaa tgaaggaaaa gataatcgag gtcttttatc cttatcgcaa gtgtatcccc
     4621 ttgtgttagg taatttggga gcaggcaata gcacaatgaa agcggttttt gatggtcttt
     4681 tcacccgcgt catgctggat aaaagtttta ttcagcaaca aatcacgcac cgctccttcg
     4741 aaccgtttat tcgtgcgatc caggcgcaaa tttccccttg ttgcaattgc atcattgcgg
     4801 gaggcattga tacggcagaa atattggcac agattacccc cttcgatttc catgctctcc
     4861 agggatgcct gtggcctgcc gttcccataa atcagataac gacgttggtt cagcgataac
     4921 ccttctgttt gctggtgttt aagacgagag taaccgtcta cactatcaaa caggaggatc
     4981 tatgaccctg tcttttgtta cccgctggcg cgatgaattg ccagaaacct atacagcact
     5041 ttcccctacg cctttaaata atgcccggct gatttggcat aataccgaac tggctaacac
     5101 gctgagtatt ccatcgtcgc tgtttaaaaa tggcgcaggt gtctggggcg gcgaagccct
     5161 actgcctggt atgtcaccac tggcgcaggt ttacagtggt catcagttcg gcgtctgggc
     5221 gggccaactg ggtgatgggc gcggcatttt actcggcgaa caactgcttg ctgatggcac
     5281 tacaatggac tggcatctga aaggtgctgg cctgacgcct tattcgcgaa tgggtgatgg
     5341 acgggcggtt ttacgttcga cgatacgaga aagtctcgcc agtgaggcga tgcattatct
     5401 gggcattccg acgacccgcg cgttaagtat cgtcaccagc gattcgccag tgtatcggga
     5461 aacggcggag ctaggcgcga tgctgatgcg tgtggcacca agtcatctgc gctttggtca
     5521 tttcgaacat ttttactacc gccgcgagtc ggaaaaggtt cgtcagttag ctgactttgc
     5581 tattcgtcat tactggtcac atcttgaaga tgatgaggac aaataccgtc tctggtttag
     5641 cgatgttgtc gcacgtaccg cgtcgttaat tgcccaatgg cagacggtcg gctttgcaca
     5701 tggggtgatg aataccgaca acatgtcgct gctgggactg acgcttgatt acgggccgtt
     5761 tggttttctt gatgattacg aacccggttt tatttgtaat cactcggatc atcaagggcg
     5821 ttatagcttt gataatcaac ctgctgtcgc gttgtggaat ttacagcgtc tggcgcagac
     5881 attgtcacca tttgttgccg tagatgccct gaatgaggcc ctggacagct atcagcaggt
     5941 tttgttgacg cattatggac aacggatgcg gcagaaactg ggcttcatga cggagcaaaa
     6001 agaggataac gcgctactga atgaattatt cagtctgatg gcgcgagagc gcagcgatta
     6061 tacccgcaca ttccgcatgc tgagtctgac cgagcagcac agcgcggcgt caccgctacg
     6121 tgatgagttt attgatcgtg cggcatttga tgactggttt gcccgttatc gggggcgttt
     6181 gcaacaagac gaggttagcg atagtgagcg tcagcaactg atgcaaagcg ttaacccagc
     6241 tctggtattg cgcaactggt tggcgcaacg ggcgattgaa gcggcagaaa agggtgatat
     6301 gatggaattg caccgcctgc atgaggcgct gcgaaatcct ttcagcgaca gagatgatga
     6361 ctatgtcagt cgtccacctg actggggtaa acggctggaa gtcagctgct cgagttaaag
     6421 gctacttagt taacagcagc ttgccagcct gagttttacg gagcagatat tcttgcccgt
     6481 catgatcgat aatcagtttg ccatccggac ctaacagtgt ctggctggta attcgccgga
     6541 tgggctgcgg ggaagcggtc ttgtgattgg catccgtttc aggcgtgagt tttctgctat
     6601 ccgtataacg cataaaaatt aataataatt gatataacaa tgattcttat tatcaatatt
     6661 atggcaggcc agagcaagca gtaatgtgtt gagaagacaa caagttgcgc agaaatgccc
     6721 atcccatcag gaatgggcac gcattcagaa gcgggtatct accgcagagg cgagtttttc
     6781 gaccaggcgt tcggtatctt cccagcccag acacgggtcg gtaatggatt gaccgtaagt
     6841 gagcggctga ccgccgacga ttttttgcgt tccttcgcgc aggaaacttt ccgccataat
     6901 tccagcaatc gccgtagagc cattgcggat ttgctgacaa atatcctcac aaacttctaa
     6961 ctggcgacgg tgctgcttct ggcagttacc gtggctgaaa tccaccacca gatgttcagg
     7021 taaatcaaac tcgtgcagcg tatcgcaggc tgcggcgata tcatcggcat gataattcgg
     7081 ttttttgccg ccacgcataa taatgtggcc atacgggttg ccgctggtct gatagatggt
     7141 catctgacca tttttgtctg gcgagaggaa catatggctg gcgcgggctg cgcggatagc
     7201 atccacggca atccgcgtgt tgccatcggt accattttta aaacctaccg gacaggagag
     7261 tgctgaagcc atttcgcggt ggatctgact ttcggtagta cgagcgccaa tcgcgcccca
     7321 actgattaaa tcagcaataa actgaccggt caccatatcg agaaactcag tcgcggtagg
     7381 gacacccagc tcatttacct gtaaaagtaa tttgcgcgcc agctccagac cgtgatttac
     7441 ccgatagctg ccgtttaaat ccggatcgga gattagccct ttccagccga caacagttcg
     7501 tggtttttca aaataggtgc gcattacgat ttccagccgt gactggtact ggttgcgcag
     7561 cgactgcaga cgggtggcgt actccattgc agcggtgaga tcgtggatcg agcaggggcc
     7621 aataatgacc aacagtcgct tatcttcacc attcagtatt ttttcaattc tgcggcggga
     7681 gtcggtgaca tgggtggcga cgccaggcgt tacgggatac cgtagcgcga gttcggcggg
     7741 cgttaccagg ctctcaatac gcgcagtacg gagttcgtca gttctgttca ttacaagtct
     7801 cagaaatttg ttgcttctac ggcaggacta ccgggaagtg atgcgcatca cgataaacca
     7861 atcgcagctg atttcaagtg cgaggtgtgt cacacctcgc cgggtggtta gcatggtaac
     7921 aaaaaaacaa accaacgcac tagttctcta gtacattcgg cgactaaggc ccatgatatc
     7981 gaggatcttg gtggcaatct cttctaccga ataattggta ctgttaatcc acgggatctg
     8041 atttttacgg tacaaggctt ccacttccgc gacttccatt ctgcactgac gaagcgaggc
     8101 atagcgactg ttctcccgac gttcctcgcg aatcgccgcc agacgttccg ggtcgatagt
     8161 caggccgaac aatttatgct gaagtggttt gagcgacgcg ggtagcacca gattatccat
     8221 atcgtcggca ataaaggggt agtttgccgt gcggatacca aattgcattg ccagatacag
     8281 actggtgggg gttttaccac agcgagaaac accgagcagg atcacctgag cctgatccag
     8341 attgcgcaac gaaatgccgt catcgtgggc aagggtgtaa tcaatcgccg caatgcgcgc
     8401 atcatattta ttgagattat tagggttaag gccatgggta cgatgagcaa tcggcgttgg
     8461 atccagtttc atctcttgtt gtagcggggc aaccagcgcc tgaacgatat cctggcaaaa
     8521 gccttcactt tgcaagatga tggcgcgaat ctccggcaac acgatggagt agaagaccag
     8581 cgggcgcaca cctgtctggt gataaattgc gtcaatctga tccttcactg cccgtgcacg
     8641 gctctcattt tcgacaaacg gcagcgtaat gctgctgata gtgacgggaa attgtgacat
     8701 tactgcgtgt cctaatacct ccgcagttat tgccgtacca tcagaaatat aaaaaacgtg
     8761 gcgatcaaca gcattatcca ttttgttctt cccgtgatgc agacataatt ctatgaaagc
     8821 ataaattaaa aacgcacaga agcgtaggag gtaatgtctg gtttataaaa tgaaccttca
     8881 attttatttt ttatgaaaac agcatttcat ttttatggtt tcgtttatac cgatggttta
     8941 tgtggaaatt gtcgaataga gcaggtttgc gcaacgc
//
LOCUS       DAJTNA010000149         8872 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTNA010000149 DAJTNA010000000
VERSION     DAJTNA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.151"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..927
                     /locus_tag="OO161_003135"
                     /pseudo
     CDS             1..927
                     /locus_tag="OO161_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
     gene            complement(1018..2061)
                     /locus_tag="OO161_003136"
     CDS             complement(1018..2061)
                     /locus_tag="OO161_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5464112.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(2187..2762)
                     /gene="nudK"
                     /locus_tag="OO161_003137"
     CDS             complement(2187..2762)
                     /gene="nudK"
                     /locus_tag="OO161_003137"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5464113.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLID"
     gene            complement(2830..4809)
                     /gene="aegA"
                     /locus_tag="OO161_003138"
     CDS             complement(2830..4809)
                     /gene="aegA"
                     /locus_tag="OO161_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5464114.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            5015..6715
                     /gene="narQ"
                     /locus_tag="OO161_003139"
     CDS             5015..6715
                     /gene="narQ"
                     /locus_tag="OO161_003139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR5464115.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            6879..8872
                     /locus_tag="OO161_003140"
                     /pseudo
     CDS             6879..8872
                     /locus_tag="OO161_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT         2047 a         2376 c         2323 g         2126 t
ORIGIN      
        1 ctcacggaaa gtcatactgc cttacgggcc gatgctgcct gagttgctcg gcggttcggc
       61 ggatctggct ccagcaacct gaccatctgg aaaggttctg tttcgctgaa ggaagatccg
      121 gcgggcaact acattcacta cggggtgcgt gaatttggca tgaccgctat agccaacggc
      181 atcgcgcacc acggcggctt tgtgccgtat accgcgacgt tcctgatgtt tgttgaatac
      241 gcccgtaacg cctcgcggat ggcggcactg atgaaagcgc ggcagattat ggtttatacc
      301 cacgactcaa ttggcctggg cgaagatggt ccgacgcacc aggctgttga gcaactggcc
      361 agcctgcgct taacgccaaa tttcagcacc tggcgaccgt gcgatcaggt ggaagcggcg
      421 gtgggctgga agctggcggt tgagcgccac aacggaccga cggcactgat cctctcaagg
      481 cagaatctgg cccaggtgga acgtacgccg gatcaggtaa aagagattgc ccgtggcggt
      541 tatgtgctga aagacagcgg cggtaagcca gatattattt tgattgccac cggttcagag
      601 atggaaatcc ccctgcaagc ggcagagaaa ttagcgggag aaggtcgcaa tgtacgcgta
      661 gtttccctgc cctcgactga tattttcgac gcccaggatg aggaatatcg ggagtcggtg
      721 ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag gtattgccga ttactggtac
      781 aagtatgttg gtttgaaagg ggcgattgtt gggatgacag gttatgggga atctgctccg
      841 gcggataagc tgttcccgtt ctttggcttt accgccgaga atattgtggc aaaagcgcat
      901 aaagtgctgg gagtgaaagg tgcctgatgg tgatcgccgg atgctgattg ccggacgctg
      961 attgccggat gcgactctga cgcgtcttat ccggcctaca tgtccccgcc attttgttta
     1021 acgggtgatc cacaacgtgg gccaggcgtc tggcccatgc cagttatcgc aggtgggttc
     1081 tgcggcgtaa cgcaccaggc gaaaacgctg accgtcaaag cgccagcgcg cctgaatgcc
     1141 acaatcgctt aatccgcgcc ctttcgctaa ggtcaccagt tcacgcgatt tctcatcaaa
     1201 tgttgcgttc atcagttcca gttcattcgt ctcctgcccg ttgttgaacg gcaaacgcaa
     1261 ccgaaccggg cgcgaggcca gcggattttt acgcgacaca atccatgcca aatcaatggt
     1321 gttataggcc cctgcctcac agctaatcat catcagcgct ttatcatcag tcagcgcagt
     1381 gacattcacc tcacgacgca atggatcaag cgagcagcgc agaccattca tccgccagtt
     1441 tccataatcc agcaaatcgt tgcgttcttc gagtgagagt ggcgtcggcg ttgggttaac
     1501 caccgcgacc tctttcagcg caggcgcggg cggtacgctg agcggcggtt cgtccccttt
     1561 cttgatccac gcggtttcac tgccaacgcg cttttgctga gcatcaataa acaacaacgc
     1621 cgcttttaag ccactcagcg aaatggtctg attgccgtcg cgtaaggtga tcgccttccc
     1681 ttcctgaatc atctgcaaaa acgcggtgat ggttgccgta tcatcggtta ctaataacca
     1741 tggtgaaatc cgccacttgt caccacttaa cgccaacggc tcgccatcta acagcagccg
     1801 tggcgctatc tccccttctg acgcctccgg cgacttcaat ccgccgcgct caatacgtaa
     1861 aacggcatcg gtatgcgccc cggcgctgcg gctcagggtc atcaccagtc cattatgatc
     1921 gcccgtatta cgcgccacgc agaagttttg gttattacag gtgacctgcc agtcggaaaa
     1981 cgcccgttgc gctggtgccg cccacaccaa agacgtcggc aacaaagcga aaaaaaagag
     2041 aaggaaaatg cgatagcgca tggacggtac gaccccagaa gttcaaacaa acagcacaag
     2101 tcgtatcttc gttgcgcagg tagcgcggct caatcggatt tgtcggattg agccaataat
     2161 aaacaacgaa atattattgt tatttttcag tctattaaat gtgacgtttg caaatagtta
     2221 agcaataaca ccgtcttacc gtcacgtatc tcgccggttt tgatcatctc cagcgcctgg
     2281 ctgaacggca gctcgagcac ttcaatatct tcatcttcga caccgccacc ggcgttagcg
     2341 cgctgattgt cactgtattc ggcgataaaa aagtggatta gctcagtcac accgcctggc
     2401 gacatataca gttcaaataa tttgcgcact tcgcccactt catagcccgt ctcttcaatc
     2461 gcttctttac gaatgcacac ttccggttcg tcgttatcca gcagcccggc gcaggtttca
     2521 atcagctgcc cgctttcatt gccattaacc caggtagcga cacggaactg acgaatcaga
     2581 accacggtct ttttcttcgc gttgtacagg aggatcgtcg caccattacc gcgatcgtaa
     2641 acttcacgtt tatggcggat aacttcgcca tctttgcgcg tgagatcgta ggtaatgttg
     2701 tgcagggtga aatagttatc ggagagaatt ttgtctttaa tgagggtgat ttgttgcgtc
     2761 ataccgactc cacagcgcga aatgaacaat tatcttacgc tgtgaagtcg ggtttgtctg
     2821 cgcaggctat cagtgagatt tgactgattt tacccccagc cagtcaataa tcccctgtgc
     2881 cgcatgacgt ccttctgcca ttgcggtaac caccagatcc gcaccacgca cggcgtcgcc
     2941 accggcgaag attttcggat tggtggtctg gtaacggtac tggctttcca catccgcgat
     3001 gatgcggccc catttgtcta ccgttacacc gtgcgactcc agccacggca tcccgtgcgg
     3061 attgaagcca aacgccataa tcaccgcgtc ggctggcatg acaaattcac tgccttccac
     3121 cggtactgga cgccgacgcc cctgggcatc cggctcgcca agacgcgtgc gcaggaaacg
     3181 aatcccgcaa acgtgacctt gttcattcag ctcaagcgcc accggctgga cgttaaattc
     3241 gaagttggcc ccctcttcgc gggcgttctt cacctctttc ttcgagcctg gcatgttagc
     3301 ttcatcacga cgataagcgc aggtgacgtt actcgcgccg tggcgcagcg cagtacgcac
     3361 acagtccatc gcggtgtcgc cgccgcccag taccacgacg ttaagtccgg cggtattgat
     3421 aaacggctct tccggtagct cttcgagccc catcacctgt ttagtgttgg cgatgaggaa
     3481 cggcagcgcg tcataaacgc ccggcgcatc ttcattgggt aaacccgctt tcatggaacg
     3541 gtaagtgcct acgccaacga agaccgcgtc gtattgttcc aaaagcgaat ccaaagagac
     3601 atctttaccc acttcacaat tgagttcgaa gtgaatcccc atcgcgctga agatttcccg
     3661 acggcgtgcc agcagggatt tatccagttt gaaagaagga atgccgaaag tgagcaagcc
     3721 accgatttct ggatggcgat cgtacaccgt caccgccacg ccattgcggg tcagaacatc
     3781 cgcacaggcc agccctgccg gacctgcccc gataatcgcc acccgcttgt ccactttggt
     3841 gacatgactt aagtcaggac gccaaccttt cgccaacgcc tgatctgaaa tatagcgttc
     3901 aatgttgccg atagttaccg cgccgtgctc atcgcgaata gtacaggcac cttcacacaa
     3961 acggtcttgc ggacaaacgc gtccggtaat ttccggcagg gtgttggtct ggtgagaaag
     4021 ctcgacggcg gcgtcgatgt ttccggcttt caccagttca atccactgcg gaatatggtt
     4081 atgcagcggg caggtccatt cacaaacgct gtgctcgccg cacttaaggc agcgcgaggc
     4141 ttcccgttgt gcctggtcgg cgcgaaatgg cagataaatt tcatcaaaac cggttttgcg
     4201 cgcttcaatc gccagtttat ccggctcgcc acgcgcgggc gttgcctgca tttgttcgac
     4261 tttactcatt accggcattt cttgtgcagc ggtactggca tgccacggtt gatgctcctg
     4321 acgtgcggtg cgcaagcggc gggatttcgc catgccggag agtgcgacgt cagtgaccag
     4381 ttgcagcgcg tccgccgggc aattctcgac acaggcagga ccgttttcgc gccctgcaca
     4441 aaggtcgcat ttatgcgccg tggcttttac ttttcctgcc gcgacgggtg tcaggacgat
     4501 ttgcatcgta ccaaacggac aggccaccac gcaggattta cagccaatac acttttgctg
     4561 attgacctga atgctgtcat caacgtggct gattgcgcca ttagggcagc tacgggcgca
     4621 gggcgcatct tcacaatggt gacaggtcac tgcactacgt tgctgctgat gtttgataac
     4681 cgtaattcgg ggatgaaaat ggtgttggct caggacatgt tgctcatcat tgtgagccat
     4741 gacacaggcg atttcacaag catgacaacc cagacattgc tgactgttgg ccataataaa
     4801 acgattcata acgaccttct tttttggttg taaaaacctt attctttata tgagtgttgt
     4861 tattacccga cttacagggg aatcggcaat gttcatgtgc ccagaataag taactatttc
     4921 gctataaact gtagcagctc aaataatccc atcactgact aaattgcgtt tcagcgaact
     4981 ggaacattaa tgattttttg tggagaagac gcgtgtgatt gttaaacgac ccgtctcggc
     5041 cagtctggcc cgggcctttt tttacattgt gctgctgtcg attctttcca cgggtatcgc
     5101 tctgctaact ctggcgagca gtttgcgcga cgctgaggct atcaatattg ccggatcgct
     5161 gcgtatgcag agttaccgcc tgggctacga cttgcaaagt ggcagtccac aactcaatgc
     5221 acatcgccag ttgtttcagc aggcactgca ttcaccggta ttaaccaatc tcaacgtctg
     5281 gtatgtgcca gaagcagtaa aaactcgcta tgcgcatctg aatgccaact ggctggagat
     5341 gaataatcgg ctcagcaagg gcgatttgcc gtggtatcag gccaatatta ataattatgt
     5401 taatcagata gacctgttcg tgctggcttt acagcactac gctgaacgca aaatgctgct
     5461 ggtagtggcg atttccctgg ctggcggtat cggtattttc acgctggtct tttttacttt
     5521 gcgccgtata cgccatcagg tggttgcccc gctgaatcag ctggttaccg ccagtcaacg
     5581 tattgaacac gggcagttcg actcgccgcc gctggatacc agtctgccga atgagcttgg
     5641 tctgcttgca aaaaccttta accagatgtc gagcgagctg cataaattgt acctttcgct
     5701 ggaggcgtca gtagaagaaa agacccgcga tctccacgag gccaagcgtc gtctggaggt
     5761 gttgtatcaa tgttcgcagg cgctgaacac aagtcagatt gatgtgcatt gtttccgcca
     5821 tattttgcag attgttcgcg acaatgaagc ggctgaatat ctggagttaa atgtcggtga
     5881 aaactggcgg attagcgaag ggcaaccaaa cccggaattg ccgatgcaga ttttaccggt
     5941 gacaatgcaa gagacggttt acggcgaact gcactggcaa aatagtcacg tttcatcatc
     6001 agaaccgctg cttaacagcg tttcgtcgat gctgggacgc ggtttgtact ttaatcaggc
     6061 gcagaagcat tttcagcagt tattgttgat ggaagaacgt gcaaccatcg cccgcgaatt
     6121 gcacgactcg ctggctcagg tactttctta cttacgtatc cagttgacgt tactgaagcg
     6181 ttcgataccg gaagataacg ccaccgcaca aagtatcatg gccgattttt cccaggcgtt
     6241 gaatgatgct tatcggcagt tacgcgagct gttgactact ttccgcctga cgctgcagca
     6301 ggcggatctc ccctccgcgt tgagggaaat gctggatacg ttacaaaatc aaaccagcgc
     6361 caaactgacc ctcgactgcc gtctgccaac ccttgcgctg gatgcgcaaa tgcaggtgca
     6421 tttgttgcaa attattcgcg aagcggtgct gaatgcgatg aagcacgcca acgccagcga
     6481 aatcgccgtc agttgcgtca ccgcgccgga cggcaatcac acggtttata tccgtgataa
     6541 cgggattggt atcggtgaac cgaaagaacc cgaaggtcat tatggtctga atatcatgcg
     6601 cgaacgcgcg gaacggctgg gtgggacgct gactttttca caaccttccg gcggcggcac
     6661 gttagtgagt attagctttc gctctgcaga gggtgaggaa agtcagttga tgtaatgcct
     6721 cctactgacc aaagaatact tgcacttaag gttcagtata aaagggcatg ataatttaca
     6781 ttaactcctt tttttctcca cgattggctc gtaccttgcc gctacagtga agcaagtcaa
     6841 gcctacaacg atacgcagaa acacgaggtc ctcttttaat ggcgaatttc tttattgatc
     6901 gccccatttt tgcctgggtg ctggcaatcc tgttgtgtct gacagggacc ctggcgattt
     6961 tttcattgcc cgttgaacaa taccccgatc tcgcgccacc gaatgtgcga gtgaccgcta
     7021 actatcccgg cgcatcggcc cagacgctgg aaaacaccgt gccccaggtt atcgagcaaa
     7081 atatgaccgg cctcgataat ctcatgtata tgtcatctca gagcagtggc accggtcagg
     7141 catctgtcac tttaagtttt aaagcaggca ccgatccgga cgaagccgtg cagcaagtac
     7201 aaaaccagct gcaatcagcc atgcgaaagt taccgcaggc ggtgcaaaat cagggcgtga
     7261 cggtgcgtaa aaccggcgat accaatattc tgaccattgc tttcgtctct accgatggtt
     7321 cgatggataa acaggatatt gccgattatg ttgccagtaa tattcaggac ccgttaagcc
     7381 gcgtgaatgg cgtcggggat atcgatgcct atggttcgca atattccatg cgtatctggc
     7441 tggccccggc gaaactcaac agtttccaga tgacggctaa agatgtcact gatgccattg
     7501 agtcacagaa cgcgcagatt gcggttgggc aacttggtgg tacaccttcc gtcgataagc
     7561 aggcgctcaa cgccaccatt aacgcccagt cactgctgca aacaccagaa cagttccgcg
     7621 atatcacctt gcgggtgaat caagacggct cagaggtaag gctgggcgat gtcgccaccg
     7681 tcgaaatggg ggcggagaaa tacgattatc ttagccgctt caatggtaag ccagcctccg
     7741 ggctgggggt aaaactggcc tccggcgcaa acgaaatggc gacagggagc tggtgctcaa
     7801 tcgtctcgac gagctggcgc agtacttccc gcacggactg gaatacaagg tggcgtatga
     7861 aaccacctcg tttgttaaag cctccattga agacgtggtg aaaacgctgc tggaagctat
     7921 cgccctggtt ttcctcgtta tgtatctgtt cctgcaaaac ttccgcgcca cgctgatacc
     7981 cactatcgcc gtgccggtgg tgttgatggg aaccttctcc gtactttacg ccttcggtta
     8041 cagcgtcaac accttaacca tgttcgcgat ggtactggcg atcggtctgc tggtggatga
     8101 cgcaatcgtg gtggtggaaa acgtcgaacg tattatgagt gaggaaggac tcactcctcg
     8161 cgaagccaca cgtaaatcga tggggcagat ccagggggca ctggtcggga ttgcgatggt
     8221 gctttcggcg gtatttgtac caatggcctt cttcggcggc accaccggtg ccatctatcg
     8281 ccagttctct attaccattg ttgcggcgat ggtgctgtca gtactggtag cgatgatcct
     8341 cactccggct ctgtgtgcca cactacttaa gccactgaaa aaaggtgagc atcacgggca
     8401 aaaaggcttt tttgcctggt ttaaccagat gtttaaccgc aacgccgaac gctacgaaaa
     8461 aggggtggcg aaaattctcc accgcagcct gcgctggatt gtgatttatg tcctgctgct
     8521 tggcggcatg gtgttcctgt tcctgcgttt gccgacgtcg ttcttaccgc tggaagaccg
     8581 tggcatgttt actacctcgg tacaattgcc cagcggttca acccaacaac agaccctgaa
     8641 agtcgttgag caaatcgaga aatattactt cacccatgaa aaagacaaca tcatgtcggt
     8701 gtttgccacc gttggttctg gccctggggg taacgggcaa aacgtggcgc gaatgtttat
     8761 ccgcctgaaa gactggagcg aacgcgacag taagaccggc acctcgtttg ccattatcga
     8821 gcgtgcaaca aaggcgttta acaagattaa agaagctcgc gttatcgcca gc
//
LOCUS       DAJTNA010000150         8855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTNA010000150 DAJTNA010000000
VERSION     DAJTNA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.152"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..322
                     /gene="tnpA"
                     /locus_tag="OO161_003141"
                     /pseudo
     CDS             1..322
                     /gene="tnpA"
                     /locus_tag="OO161_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(453..1776)
                     /locus_tag="OO161_003142"
                     /pseudo
     CDS             complement(453..1776)
                     /locus_tag="OO161_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1776..3392)
                     /locus_tag="OO161_003143"
     CDS             complement(1776..3392)
                     /locus_tag="OO161_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5464116.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3688..4404
                     /locus_tag="OO161_003144"
     CDS             3688..4404
                     /locus_tag="OO161_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5464117.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEFQVEYHLRRLHPEKS"
     gene            complement(4401..6062)
                     /locus_tag="OO161_003145"
     CDS             complement(4401..6062)
                     /locus_tag="OO161_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5464118.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALSLGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6258..6686)
                     /gene="ibpB"
                     /locus_tag="OO161_003146"
     CDS             complement(6258..6686)
                     /gene="ibpB"
                     /locus_tag="OO161_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5464119.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6798..7211)
                     /gene="ibpA"
                     /locus_tag="OO161_003147"
     CDS             complement(6798..7211)
                     /gene="ibpA"
                     /locus_tag="OO161_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5464120.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7205..7277)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7517..7849
                     /locus_tag="OO161_003148"
     CDS             7517..7849
                     /locus_tag="OO161_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5464121.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7851..>8855)
                     /locus_tag="OO161_003149"
     CDS             complement(7851..>8855)
                     /locus_tag="OO161_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5464122.1"
                     /translation="PKSEKYVFIHGPENPDETWYYDFHHRRGVIAEGGKVSNLDAMDI
                     TAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNVGVAAPFGPVNVQ
                     KQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAFIGDTLSPKGEKV
                     PELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTHHRVYPGLVNVPR
                     HWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQSAFNWHPSGEWL
                     GFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWMEGGQLWITETDR
                     "
BASE COUNT         2253 a         2337 c         2219 g         2046 t
ORIGIN      
        1 tgactatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg ctgattttga
       61 agctgaactg gtttaaatgg atggcgaacc agatcacgtc catttgttaa ttaactatcc
      121 tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg gtaggtgact
      181 gcgacgagat ctaccagata ttgcagtcag gtattactac aaaggcgttt tgtggagtcc
      241 tggctatttt gccaatagct gcggaggtgc gccaatatcc gtcatccgcc aatacattga
      301 acaacagcaa acacctggtt aggtggaaaa ccgcgcctta tatccccgcc ctgaaggacg
      361 gggttttacg gcgcaccgga taaatttggt tatgcgccgt cagccgtgct aaagaatacg
      421 gaccagcacc caacggatgc cagtccacac aattaacgta actctggcca gtacgctttg
      481 ttggcctcca gcaactcatc cagaatagct ttggcgaccg aggcgctcgg taccgttttc
      541 gataacgtaa tcgcctgcca caggtgctga tatgaacgct gttcccaggc gtccacgacc
      601 agtttttcca ccgccacttg ctgactcatt aaccctttct gaaactgcgg gatatcgccg
      661 accaccagtg gttctggtcc gttgtgcccg accagacacg ggatctcgac catcgcttca
      721 tcatcaaagt tatggattgc cccgttgtta ggcacaatca gcaacatccg ctcctgagtg
      781 ttgaaggcaa ttgccgccgc cagatcgacg atgtatgacg catgttcgtc aatctccagc
      841 ttggccgccg ctgaatttcc tgccgctgta atggcgcggc aagcatcgaa aacctgtttc
      901 tcgcgatgtt ccattcacct cattcgcgcg ggtatgttca gggttggaat gctgaacaac
      961 ataatccggg aagagataat atttcagata ggtgttcggc aatgtatccg ggtccagtgc
     1021 ctggacatcc cgcgcttttg cataggtgtc gttccagctc gcttcagtat gtgtatcttg
     1081 ctgcggtgga acataaccgt gcttagagac atattgccgc agctggggca tcaggtcgtt
     1141 gccctgcaaa tcttcaattg atgtccacca gccaaagtgg ttcaggccgt agtagcgcac
     1201 gcgcatctgt ttgcgatctt gcagcccaat aatttgcgcc atccggcttt caataccgat
     1261 tggcatgtca cagatgttga ggattttcgc attcgggcgc agacgacgcg tggcttctgc
     1321 gacaattgct gccgggttgg agtagttgag catccaggca tttggtgaat atttttccat
     1381 ataatccacc agttccagga cgccgccaat ggaacgcatg ccgtacgcta ttccgccggg
     1441 tccgcaagtt tcctgaccaa caacgccgtg gcgcagcggg attttttcat ccagttcgcg
     1501 catcgggtat ttgccgacgc ggatgtgcgc catgacaaaa tcaacgtcgc tgaatgccac
     1561 ttcaggatcg gtggtgtaac taaacgcaat gtccggcgct ttctctttaa ggatgacttt
     1621 acaggcttcg gcaatcactt cctgccgcgc accatcgtta tcataaaatt tcagtgcacg
     1681 aagcgggaaa cggtcctgat tcgccaggag catcaacacg atgcctggcg taaaggtgct
     1741 tccaccgcct gcgacaacca ctgagaattt ggtcattata ctgcctccgt aatggcaaca
     1801 ttttctgctg attgatgaga attaattaag ctatcgagct gttcacgcag ctgggataca
     1861 tgcagaccaa tgatcacctg aatggcatcg ccactacgga agacgccgtg cgctcccagc
     1921 tttttaaaga cttcgtcatc cagcgtttgt gacatgtcat gcagtgcaat acgtaaacgc
     1981 gtcgcgcaat tgttaatgct ggagatattg ccgaccccgc ccagggcttg caggataccg
     2041 gcagcctgat ccagctcttt ttttggctct gcagcggtgg tttggcctcg cgaggctttg
     2101 tattcggctt ttgagtagag tttcacttcc gcatcttcac gtcccggcgt gcacatgttg
     2161 aattgcagaa tcagtgtgcg gaaaaccacg aagtacagca gggtaaagca caacccaatg
     2221 gcgatttggg tcagcatcat atccgcgtgg ttgctgaaca tcgggatcca gttttgcggt
     2281 aaaacctggt caatcagacc tccgcccatg ttgcccacca caccaaagag atacatcacg
     2341 gtcgacattg aggccgccag cacggcgtgt accgcaaaca gcaacggtga aatgaacagg
     2401 aaggtaaatt ccagcggttc ggtaattccc accagcatgg cggttaaggt tgcgggaatc
     2461 agcaagcccg ccacttttac ccgattttcc ggtgcggcag tgaagtacat cgctaaagaa
     2521 atgcccacgg caccaaagat ttttgagtta ccgtgcaggg caaaaccgcc ttccgggaac
     2581 aacgatttca gcggctcggc gctcagactg aactcttgca gatgctgcgc ccagtacatc
     2641 tgaatgccgc cttcaacagc tgccggacca aagataaact gtccgtagat gaagtggtgt
     2701 aaaccggttg ggatcagaat acgttcgagg aaggtgtaaa cccacacccc aagcgcaccc
     2761 gccgaacgca ggaacgcttg cagagattca atccccattt gtacttttgg ccagccgagc
     2821 aacgtcagcc aggcacaggg gatcatcacc aggaaggcga taatcaccac ataagacgtc
     2881 ccctggaaaa tgccgagaaa aaccggcagt tttttatcga acagacggtt atgcagcgcc
     2941 gtcacaatgc cggaaatgat aattgcgccg ataatgctgg tatcgagggt tttaatcccg
     3001 gccatcattg tcagaccgct acctgccacc gcgtcctgag tgaaatcgac gccgaagtag
     3061 cttccccagg tcattcccat cgcgttgatg aaatagttcc aagtcaggaa actcaccatc
     3121 accgccagac aagcacgccc ctgcgcttgc ttagcaaggc caatgggtaa accgacagca
     3181 aaaatcagcg gcatattacg gaataccgtc caaccgccct cttcaataat gtgtacgatt
     3241 tgcgcgaata aactgttcgg atcggtcagt gattccccga caaacatcgg gttttgcagc
     3301 aagatggcaa gacccaccac aatcccggcg aagggaaaca gcagcactgg cgtgaacatc
     3361 gcgccgccaa agcgttgaat ttgactgagc atttccacat cctcttttct caattctgta
     3421 agtaggaagc ctgattggtt atgcggcagg cctggagcga ggataggaag atgcgatcgg
     3481 cagaacatga cgacccaatg ggattcgtga tcgctttcat gcttttcgtt gggtggtaga
     3541 gatctacgtt ttcgcgaaaa agtagacatc tgtgagcact ggtacacagc atcgaacaca
     3601 tctttaaaaa aaagatgttt tttcaatcga ttaagcagaa cttgtgggcg cattacccgg
     3661 gcttgcaggc aaaaaagaga tctagagatg atctacaaaa gcattgcgga gcggttaaga
     3721 attcgactta attccgcgga tttcacgcta aacagccttc ttcccggtga aaaaaagttg
     3781 gcggaagaat ttgcagtatc ccggatgacc atccgtaaag cgattgacct gctggtagcg
     3841 tgggggctgg tggtccgccg ccacggcagc ggcacttacc tggtgcgcaa agatgtgctg
     3901 catcaaaccg ccagcctgac cggactggtg gaggtgttaa aacggcaggg aaaaacggtc
     3961 accagccagg tgctgatttt tgaaatcatg cctgcgcctc cggccattgc cagccagtta
     4021 cggattcaaa tcaacgagca gatctacttc tcccgtcgcg ttcgttttgt ggaagggaaa
     4081 ccgctgatgc tggaagacag ctatatgccg gttaaactgt tccgtaatct ttcgctgcaa
     4141 catctggaag ggtcgaagtt tgaatatatt gaacaagagt gtgggatttt gattggcggt
     4201 aattatgaaa gcctgacgcc ggtgctcgcc gatagactgc tggcgcggca aatgaaggta
     4261 gcggaacaca cgccactgct gcggatcacc tcgttgtcat atagcgagag cggggagttt
     4321 ttgaattatt cagtgatgtt cagaaatgcc agcgaattcc aggtggagta ccatttacgg
     4381 cgactccacc cggaaaagag ttaaccgata ctccagaaga gcaccgccag taattggggg
     4441 gtgataattc gcagaaacat cactaacgga tagacagtgg cgtaagagag cgccgccgca
     4501 ccactggttg gatgaagatt attagcaaac gccagcgccg gaggatcggt catggaacct
     4561 gccagcatcc cgcacatggt caggtaattc attttggcta acatccgcgc cagaatgcca
     4621 acagtaatca gcggaacggc ggtgatcagg gcaccataac caatccagct tagcccttcg
     4681 ccattgacca gggtattcac aaaatcccca ccagatttca gaccaacgac cgagaggaac
     4741 agcacgatcc ccagctcccg cagcgcgagg ttggcacttg gcggcataaa ccagtacagc
     4801 ttgccgatac tgccgatacg cccgaggctc aacgccataa tcagcggacc gcccgccagc
     4861 cccagtttca acgcggccgg gaacccaggc acaaagacgg gaatagaacc taacaatacg
     4921 ccaagcccga tgccaataaa cactggcagc atctgaacct gttgcagttt ttgctgcgca
     4981 ttccccagca cattggcaac ggcatcaatt gcggacggac gccccaccag attgaggata
     5041 tcgccgaact gcaggctgat atcgccactg gcgaccagtt cgaccccggc acggttcagg
     5101 cgcgagataa caacgtcata gcgttcttta aagtgcaggt cgcgaatacg ttttccgagc
     5161 acgttttcat tggtgaccac cacacgctcg acgcgcaaat cagtgccttt cgtggacagc
     5221 gaagtatcga cctcctgacc aatcaccagt tgcgcattat gtaaatccgc tggctgcccc
     5281 accagatgca gcaaatcgcc cagttggata atggtatctg gcgaaggaac ttttagggtt
     5341 tcttcgcgtt tcagacgcga gcagataatt ttgtcgccgt tgagaatcgg tacatcttta
     5401 atggctaaat catgcaggtt agggttctca acgcgaatat tgatagtctt gatcagcgcg
     5461 ccgccattgg tgcgtgaaga ctcgtgctgc tgagcttctg tctcgacatt gacgcggaaa
     5521 ataacccgca acatccacat ggtgaacaaa atcccgcaaa tgccgaatgg atacgccatc
     5581 gcgtaactca tccccatctg atcgaccatt tccattggtg tacccaggtc gcgcaagatc
     5641 tgctgccctg cccccagcgc tggcgtattg gtaaccgcac cggagaaaat ccccagcact
     5701 accggcagtg gaatatcaaa cagtttatgc aggatggcgg taaccagacc accgatgatg
     5761 acgatcagaa cagcaaacag gttgaggcgt aatccggaga cgcgcaatga ggcaaagaag
     5821 cccggcccta cctgaatccc gatggtataa acgaacagga tcaggccaaa ttcctgaata
     5881 acatgcagca tatcgctact taatgtcatc cccgcctgag aaacaaaatg gccgacgatg
     5941 atcccaccaa acagcacgcc gccaatacct aatcctatgc cgcgaaattt gacgttgccg
     6001 ataaacaaac cgacgactgc caccaaagcc agaatactga ccgttaatgc tatatcactc
     6061 atcgtccatt tctcttgaga taacatttta attgctacgg attatggctg aatcggtgaa
     6121 gcagatttgc cttatagcgc acattatcac gggaaagaac gtgccgaaat atcttaaaca
     6181 gtcgaaattt aacgcattgt tgaagaagaa agacaaaaaa agccccacca gaatggcggg
     6241 gcaaagagaa tagctagtta gctatttaac gcgggacgtt cgctgatagc gatacgctgc
     6301 gctgcgatgg gttcaggctc attacgaatt aaatcaatat gcagtaaacc gtttacgaag
     6361 gttgcgccag agacttccat attttcagcc agcgtaaagc tcaggctaaa tggctgattc
     6421 ataagccctt gatgcagcca ttttttctct tcttttggct gctccggcgt gccttttacg
     6481 ctcaggcgcg taccttccag ttgaatctct aaatcttcct gacggaaacc tgccagcgca
     6541 agggtaatgc ggtagtggtt atcgtcgctt ttctcaatgt tgtacggcgg gaagctctgg
     6601 ctttcaccgg cgttttgcag tgcgttggcc agtttgtcaa aaccgatcca ttgacgcatc
     6661 agtggggata aatcgaagtt acgcatagtc atttctcctt ctaagaagcg agtaagtacc
     6721 tgcaaatccg aagattcgca tatgctccct gacggcgagc atggagatgt caggccgcgt
     6781 caggcggcct tagggtatta gttgatttcg atacggcgcg gttttttcgc ttccggaatc
     6841 acgcgttcga gatcgatata cagcaaacca tttaccaggt tagccccacg aacatgaatg
     6901 ttctcagcta actggaattt gcgttcaaag ttgcgttcag cgatgccctg gtacagatag
     6961 gtgcgctctt tttgttcgtc ggcgtgagca cctttcacca ccagcagatt atcctgggcg
     7021 gtaatttcca gttcgctctc agcaaaacca gccacagcga tagcaatgcg gtaatggttt
     7081 tcgtctacca gttcaacgtt atacggaggg tagccgccat tactctggct ctggttgttt
     7141 tctaagtggt taaacaaacg gtcaaatcca atagcagaac ggtaaagcgg ggataaatca
     7201 aagttacgca taatcaatag ctcctgaaat cagcgagaat gtaatacctt ccacaatgga
     7261 caggtcaggt agccagaaca cccatcaggc gcgtcctcat cggctacgat gtaaaaatgg
     7321 gtctggaaat gaacttttca agccttatca gcggactttt ttttgcagtt tatggtctat
     7381 tgcatagact gagggggcag cacgaaacgt taaagtgcga tagccgccac agtgcgacgt
     7441 aatgatgatg ttattttcag caagggagcg ctataactca tcaggccaac acccacgatc
     7501 aacagatgaa tttattatga taagaaatgt gttgttagcg ttcatgatat gcagcggaat
     7561 gacattactc ggaggatgct ccagcgtgat gtcccatacc ggagggaaag aaggaacgta
     7621 tcccggcacc cgcgccagcg caaccatgat tggcgacgat gaaaccaact ggggcactaa
     7681 gtcgctggcg attctcgata tgccgtttac cgctgtgatg gacacgcttt tactgccgtg
     7741 ggatgtgttc cgcaaagaca gctcggtgcg ctcacgcgtt gaaaaaagcg aagccaacgc
     7801 gcaagccacc aatgcggtta ttccaccggc cagaatgcct gataactgat ttagcgatca
     7861 gtttcggtga tccacagctg gccgccttcc atccacgcca gccattgacc atccggcgag
     7921 aagaccacgg cgtccgcaga aggtggattg gcgtggtttt cggttaaata ctcaacctcg
     7981 ccactttgcg catgggcaca agcaattcga ttatccagca caaagcccaa ccattctcct
     8041 gacggatgcc agttaaatgc agactgaata tccgttttgt tatgggttaa ctggcgcggc
     8101 tcgccgccct gtggcgagat aagccacagt tgcacaatgc cgttatcatc acgcattaaa
     8161 aacgcgattt gcgtaccctg cggattacag cgcacccagt ggcgcgggac gttgactaac
     8221 cccggataaa cccgatggtg ggtaaaggtt aaacgtcgct gcacgacgcc gcgcggtggc
     8281 gcgggcaggg tggtttccgt tccacttaac ggcgcatcac ctgccgcttt ccagccagct
     8341 tcatcttgcg gtaactcaac gataaacagc tccggcactt tctcgccctt tggcgaaagt
     8401 gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata agcacgattg
     8461 atttcatcgc tgccaggctg cggcgtgggc gtggttttac tcaccagcac gcaccagtgg
     8521 ctaccgctgt attcacgcgg atgctgcttt tgtacgttga ccgggccaaa cggcgcagca
     8581 acaccgacgt ttcgcaaatc cagcgccgga tcgagttcat gcattacatg gtcgttatag
     8641 gtaaagctca ccctttcacc gttcgggcta aagacatgca catggctgcc gccgcgcagc
     8701 gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac cttgccgcct
     8761 tcagcaatga ctccgcgtcg atgatggaaa tcgtaatacc atgtttcatc aggattttcc
     8821 gggccatgga tgaaaacata tttctctgac ttcgg
//
LOCUS       DAJTNA010000151         8766 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTNA010000151 DAJTNA010000000
VERSION     DAJTNA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.153"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(77..868)
                     /gene="aadA1"
                     /locus_tag="OO161_003150"
     CDS             complement(77..868)
                     /gene="aadA1"
                     /locus_tag="OO161_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5464123.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(981..1811)
                     /locus_tag="OO161_003151"
     CDS             complement(981..1811)
                     /locus_tag="OO161_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5464124.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2021..3034
                     /gene="intI1"
                     /locus_tag="OO161_003152"
     CDS             2021..3034
                     /gene="intI1"
                     /locus_tag="OO161_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5464125.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3237..3587
                     /locus_tag="OO161_003153"
     CDS             3237..3587
                     /locus_tag="OO161_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5464126.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3713..4273
                     /locus_tag="OO161_003154"
     CDS             3713..4273
                     /locus_tag="OO161_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5464127.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4276..7242
                     /locus_tag="OO161_003155"
     CDS             4276..7242
                     /locus_tag="OO161_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5464128.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7309..7686
                     /locus_tag="OO161_003156"
     CDS             7309..7686
                     /locus_tag="OO161_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5464129.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7887..8546)
                     /locus_tag="OO161_003157"
     CDS             complement(7887..8546)
                     /locus_tag="OO161_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5464130.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1948 a         2478 c         2348 g         1992 t
ORIGIN      
        1 ttatgtgctt agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg
       61 aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgaa
      121 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag
      181 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt
      241 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca
      301 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta
      361 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg
      421 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat
      481 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc
      541 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa
      601 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa
      661 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca
      721 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg
      781 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg
      841 atacttcggc gatcaccact tccctcatga tgtttaacgt ttgaaataag gggcgccgag
      901 cgccagcgag gggagccaaa agcttgcttt tggccgtccc gacttgattg aagggttggg
      961 cgattttgcc attagatttt ttataaattt agtgtgttta gaatggtgat cgcatttttc
     1021 ttggctttta tgcttgatgt taaattcgac cccaagtttc ctgtaagtgc ggacacaaaa
     1081 acatatttat gtcctgattt gcttataata aacccttcaa accatccgtt ttgtaaggtt
     1141 ctatttgctg tgaatcctgc accagttttc ccatacagtt ttgtactatt atccagatct
     1201 tgtagataca tgttctctat ggtgttttct atggctgagt ttttaactgg gagattgtga
     1261 ttaataattt tacgcaggaa ttgaatttgt tcttctggtg aaatttttaa gctactttcg
     1321 agccatgctt ctgttaatcc gttgtttctt tctttatctc cagagaagtc ttgatttcca
     1381 taatcaaaat ctttgagata attcttgatt ttatttaatc caattttttg ggttatttct
     1441 tgcgaaaccc aaacaacaga aaattgcatc cacgtctttg gtgtatgatt gctgttccag
     1501 atctccattc ctttgggggt tttatcccat ttgaatatgg ttttctgatc tattatttcc
     1561 gcatcaaatg ccataagtga taatgcgatc ttgaaagttg aatctggtgc catttgcgtt
     1621 gcacactttg ctttattgaa ttgagcaatt tcagcgtttg tggatgcatc gtaaagtaaa
     1681 aaacaacctt cagttccttc aaataatgga gatgcaacag tagagatatc tgttgatgca
     1741 ctggcgctgc tgtagataat atttgcaatt attaaaaaaa tagcgaagtt gatatgtatt
     1801 gtgtttttca taataagtat tggtttggta aagggcttaa ttttaacggc taacaattaa
     1861 tgaggctccg ggttcgccca actttgtttt agggcgactg ccctgctgcg taacatcgtt
     1921 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1981 aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     2041 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     2101 gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgcct tcatccgttt
     2161 ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg
     2221 gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt caggcattgg cggccttgct
     2281 gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga tcggaagacc
     2341 tcggccgtcg cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg
     2401 ttttctggaa ggcgagcatc gtttgttcgc ccagcttctg tatggaacgg gcatgcggat
     2461 cagtgagggt ttgcaactgc gggtcaagga tctggatttc gatcacggca cgatcatcgt
     2521 gcgggagggc aagggctcca aggatcgggc cttgatgtta cccgagagct tggcacccag
     2581 cctgcgcgag cagctgtcgc gtgcacgggc atggtggctg aaggaccagg ccgagggccg
     2641 cagcggcgtt gcgcttcccg acgcccttga gcggaagtat ccgcgcgccg ggcattcctg
     2701 gccgtggttc tgggtttttg cgcagcacac gcattcgacc gatccacgga gcggtgtcgt
     2761 gcgtcgccat cacatgtatg accagacctt tcagcgcgcc ttcaaacgtg ccgtagaaca
     2821 agcaggcatc acgaagcccg ccacaccgca caccctccgc cactcgttcg cgacggcctt
     2881 gctccgcagc ggttacgaca ttcgaaccgt gcaggatctg ctcggccatt ccgacgtctc
     2941 tacgacgatg atttacacgc atgtgctgaa agttggcggt gccggagtgc gctcaccgct
     3001 tgatgcgctg ccgcccctca ctagtgagag gtagggcagc gcaagtcaat cctggcggat
     3061 tcactacccc tgcgcgaagg ccatcggtgc cgcatcgaac ggccggttgc ggaaagtcct
     3121 ccctgcgtcc gctgatggcc ggcagcagcc cgtcgttgcc tgatggatcc aacccctccg
     3181 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacaatgg
     3241 aggtggtagc cgagggtgtg gaaacacccg actgccttgc gtggttgcgg caggcgggtt
     3301 gcgacacggt gcagggtttc ctgttcgcca ggccgatgcc ggcggcggcc ttcgtcggct
     3361 tcgtcaacca atggaggaac accaccatga acgccaatga accgagcacc agttgctgcg
     3421 tgtgctgcaa ggaaatcccg ctcgatgccg ccttcacgcc ggaaggggcc gagtacgtgg
     3481 agcatttctg cgggctggag tgctatcagc gcttccaggc gcgggccagc actgcgaccg
     3541 aaaccagcgt caaaccggac gcttgtgatt cgccgccgtc aggttgaggc ataccctaac
     3601 ctgatgtcag atgccatgtg taaattgcgt caggatagga ttgaattttg aatttattga
     3661 catatctcgt tgaaggtcat agagtcttcc ctgacatttt gcagggaatt ccatgactgg
     3721 acagcgcatt gggtatatca gggtcagcac cttcgaccag aacccggaac ggcaactgga
     3781 aggcgtcaag gttgatcgcg cttttagcga caaggcatcc ggcaaggatg tcaagcgtcc
     3841 gcaactggaa gcgctgataa gcttcgcccg caccggcgac accgtggtgg tgcatagcat
     3901 ggatcgcctg gcgcgcaatc tcgatgattt gcgccggatc gtgcaaacgc tgacacaacg
     3961 cggcgtgcat atcgaattcg tcaaggaaca cctcagtttt actggcgaag actctccgat
     4021 ggcgaacctg atgctctcgg tgatgggcgc gttcgccgag ttcgagcgcg ccctgatccg
     4081 cgagcgtcag cgcgagggta ttgcgctcgc caagcaacgc ggggcttacc gtggcaggaa
     4141 gaaatccctg tcgtctgagc gtattgccga actgcgccaa cgtgtcgagg ctggcgagca
     4201 aaagaccaag cttgctcgtg aattcggaat cagtcgcgaa accctgtatc aatacttgag
     4261 aacggatcag taaatatgcc acgtcgttcc atcctgtccg ccgccgagcg ggaaagcctg
     4321 ctggcgttgc cggactccaa ggacgacctg atccgacatt acacattcaa cgataccgac
     4381 ctctcgatca tccgacagcg gcgcgggcca gccaatcggc tgggcttcgc ggtgcagctc
     4441 tgttacctgc gctttcccgg cgtcatcctg ggcgtcgatg aactaccgtt cccgcccttg
     4501 ttgaagctgg tcgccgacca gctcaaggtc ggcgtcgaaa gctggaacga gtacggccag
     4561 cgggagcaga cccggcgcga gcacctgagc gagctgcaaa ccgtgttcgg tttccggccc
     4621 ttcaccatga gccattaccg gcaggccgtc cagatgctga ccgagctggc gatgcaaacc
     4681 gacaaaggca tcgtgctggc cagcgccttg atcgggcacc tgcggcggca gtcggtcatt
     4741 ctgcccgccc tcaacgccgt cgagcgggcg agtgccgagg cgatcacccg tgctaaccgg
     4801 cgcatctacg acgccttggc cgaaccactg gcggacgcgc atcgccgccg cctcgacgat
     4861 ctgctcaagc gccgggacaa cggcaagacg acctggttgg cttggttgcg ccagtctccg
     4921 gccaagccaa attcgcggca tatgctggaa cacatcgaac gcctcaaggc atggcaggca
     4981 ctcgatctgc ctaccggcat cgagcggctg gttcaccaga accgcctgct caagattgcc
     5041 cgcgagggcg gccagatgac acccgccgac ctggccaaat tcgagccgca acggcgctac
     5101 gccactctcg tggcgctggc caccgagggc atggccaccg tcaccgacga aatcatcgac
     5161 ctgcacgacc gcatcctggg taagctgttt aacgctgcca agaataagca tcagcagcag
     5221 ttccaggcgt caggcaaggc catcaacgcc aaggtacgtc tgtacgggcg catcggtcag
     5281 gcgctgatcg acgccaagca atcaggccgc gatgcgtttg ccgccatcga ggccgtcatg
     5341 tcctgggatt cctttgccga gagcgtcacc gaggcgcaga agctcgcgca acccgatgac
     5401 ttcgatttcc tgcatcgcat cggcgagagc tacgccaccc tgcgccgcta tgcaccggaa
     5461 ttccttgccg tgctcaagct gcgggccgcg cccgccgcca aaaacgtgct tgatgccatt
     5521 gaggtgctgc gcggcatgaa caccgacaac gcccgcaagc tgccagccga tgcaccgacc
     5581 ggcttcatca agccgcgctg gcagaaactg gtgatgaccg acgccggcat cgaccggcgc
     5641 tactacgaac tgtgcgcgct gtccgagttg aagaactccc tgcgctcggg cgacatctgg
     5701 gtgcagggtt cacgccagtt caaggacttc gaggactacc tggtaccgcc cgagaagttc
     5761 accagcctca agcagtccag cgaattgccg ctggccgtgg ccaccgactg cgaacaatat
     5821 ctgcatgagc ggctgacgct gctggaagca caacttgcca ccgtcaaccg catggcggca
     5881 gccaacgacc tgccggatgc catcatcacc gagtcgggct tgaagatcac gccgctggat
     5941 gcggcggtgc ccgacaccgc gcaggcgctg atagaccaga cagccatggt cctgccgcac
     6001 gtcaagatca ccgaactgct gctcgaagtc gatgagtgga cgggcttcac ccggcacttc
     6061 acgcacttga aatcgggcga tctggccaag gacaagaacc tgttgttgac cacgatcctg
     6121 gccgacgcga tcaacctggg cctgaccaag atggccgagt cctgccccgg cacgacctac
     6181 gcgaagctcg cttggctgca agcctggcat acccgcgacg aaacgtactc gacagcgttg
     6241 gctgaactgg tcaacgctca gtttcggcat ccctttgccg ggcactgggg cgatggcacc
     6301 acatcatcat cggacggaca gaatttccga accgctagca aggcaaagag cacggggcac
     6361 atcaacccaa aatatggcag cagcccagga cggactttct acacccacat ctccgaccaa
     6421 tacgcgccat tccacaccaa ggtggtcaat gtcggcctgc gcgactcaac ctacgtgctc
     6481 gacggcctgc tgtaccacga atccgacctg cggatcgagg agcactacac cgacacggcg
     6541 ggcttcaccg atcacgtctt cgccctgatg cacctcttgg gcttccgctt cgcgccgcgc
     6601 atccgcgacc tgggcgacac caagctctac atcccgaagg gcgatgccgc ctatgacgcg
     6661 ctcaagccga tgatcggcgg cacgctcaac atcaagcacg tccgcgccca ttgggacgaa
     6721 atcctgcggc tggccacctc gatcaagcag ggcacggtga cggcctcgct gatgctcagg
     6781 aaactcggca gctacccgcg ccagaacggc ttggccgtcg cgctgcgcga gttgggccgc
     6841 atcgagcgca cgctgttcat cctcgactgg ctgcaaagcg tcgagctacg ccgccgcgtg
     6901 catgccgggc tgaacaaggg cgaggcgcgc aatgcgctgg cccgtgccgt gttcttcaac
     6961 cgccttggtg aaatccgtga ccgcagtttc gagcagcagc gctaccgggc cagcggcctc
     7021 aacctggtga cggcggccat cgtgctgtgg aacacggtct acctggagcg tgcggcgcat
     7081 gcgttgcgcg gcaatggtca tgccgtcgat gactcgctat tgcagtacct gtcgccactc
     7141 ggctgggagc acatcaacct gaccggtgat tacctatggc gcagcagcgc caagatcggc
     7201 gcggggaagt tcaggccgct acggcctctg caaccggctt agcgtgcttt attttccgtt
     7261 ttctgagacg acccctatta tggcgaaggt atcattcagg aagcgttgat gaaggattat
     7321 ctctgtaaaa agttattcaa ccggctttcc ggtaccctgg tgatcagagc gcgctgcggc
     7381 aataacatta ctggcctggc atgttgcaat attctttatc cctcgccccg atacagcggt
     7441 cagctgcata ttaaagagct gtatgtttct cagtgcgatc gaaataaggg cacgggtaaa
     7501 gcgataatgc gctttatagc ccggcttgcg cttgaacagg aatgccttag ccttagctgg
     7561 aacgctgaaa aatccaaccc cggcgctaac cgtttttatc aggctctggg aggcagaata
     7621 aatgatcata tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc
     7681 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     7741 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc
     7801 gaatttctgc cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa
     7861 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt
     7921 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     7981 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     8041 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     8101 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     8161 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     8221 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     8281 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga
     8341 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     8401 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     8461 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     8521 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     8581 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     8641 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     8701 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     8761 agtgcg
//
LOCUS       DAJTNA010000152         8734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTNA010000152 DAJTNA010000000
VERSION     DAJTNA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.154"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            244..1155
                     /gene="ttdA"
                     /locus_tag="OO161_003158"
     CDS             244..1155
                     /gene="ttdA"
                     /locus_tag="OO161_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR5464131.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETATVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1152..1757
                     /gene="ttdB"
                     /locus_tag="OO161_003159"
     CDS             1152..1757
                     /gene="ttdB"
                     /locus_tag="OO161_003159"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5464132.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1806..3269
                     /gene="ttdT"
                     /locus_tag="OO161_003160"
     CDS             1806..3269
                     /gene="ttdT"
                     /locus_tag="OO161_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5464133.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3312..4325)
                     /gene="tsaD"
                     /locus_tag="OO161_003161"
     CDS             complement(3312..4325)
                     /gene="tsaD"
                     /locus_tag="OO161_003161"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5464134.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4562..4777
                     /gene="rpsU"
                     /locus_tag="OO161_003162"
     CDS             4562..4777
                     /gene="rpsU"
                     /locus_tag="OO161_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5464135.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4888..6633
                     /gene="dnaG"
                     /locus_tag="OO161_003163"
     CDS             4888..6633
                     /gene="dnaG"
                     /locus_tag="OO161_003163"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5464136.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMTYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6828..8669
                     /gene="rpoD"
                     /locus_tag="OO161_003164"
     CDS             6828..8669
                     /gene="rpoD"
                     /locus_tag="OO161_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5464137.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2108 a         2331 c         2333 g         1962 t
ORIGIN      
        1 cctgcaagtc tttggctaaa ggccagctat tcagcatcgt taattatccg cagttgtgat
       61 aagcgcagtg tatttcgcaa aacattgcca ttccctgctc tggcgcaata ttcattctta
      121 ttttcgcgtt gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc
      181 acatttttag tggtagtttg tggcggtgaa tttttccaga caaatacaaa aactggagtt
      241 gccatgatga gcgaaagtaa taagcaacag gcagtgaata agttgacaga gattgtcgct
      301 aactttaccg ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag
      361 ctaaaggatg ccgaaacgtc gtcgatgggg aaaattatct accatacgat gttcgacaac
      421 atgcaaaaag cgattgacct gaatcgtcct gcctgtcagg acaccgggga gattatgttc
      481 ttcgttaaag tcggttcccg cttcccactg cttggcgagc tgcaaagcat actcaaacaa
      541 gccgtggaag aggcaaccgt caaagcgcca ctacgtcaca atgcggtaga aatttttgac
      601 gaagtaaaca ccggcaaaaa taccggtagc ggcgtaccgt gggtcacctg ggacatcatc
      661 cccgacaatg acgatgcgga aatcgaagtt tacatggcag gtggcggctg cacgctacct
      721 ggccgctcga aagtgttaat gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc
      781 gaaaatatct ccaccctcgc cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc
      841 gccacctcgg tggaaaccgc caccgtactc tcgcgtaaag ccattttgcg cccgattggc
      901 agccgccatc ccaatccaaa agcggcagaa ctggagctac gcctggaaga aggactcaac
      961 cgtctgggga ttggtccaca agggctgact ggcaacagtt cagtgatggg cgtacatatc
     1021 gaatctgccg cccgccatcc gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg
     1081 catcgtcgcg gcacactgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc
     1141 cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata
     1201 ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgt gacgtttgtc
     1261 accgccgctt gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt
     1321 tccacgctgg ccccatcgtg cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc
     1381 cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tatcgagcag accggcgtga
     1441 aactggtggt tggcaaaggc ggaatggggc cgctgaccga agaaggctgc cagaaattca
     1501 aggcgctaca tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtggaag
     1561 agattgaaga agtgcactgg acagagctcg gaatgccgga gtcgctgtgg gtctgccggg
     1621 tcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaat ctgatagccg
     1681 aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg
     1741 tccattacat caaataaccc tcccggagag gctcgccccc tctccttttt cgcaggcata
     1801 acacgatgaa accttccact gaatggtggc gataccttgc gccgctggcg gtcatcgcca
     1861 ttattgctct aattccggtt cccgcagggc tggagagtca tacctggctc tactttgccg
     1921 tttttactgg cgtgatcgtt ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga
     1981 tggtgggtat ctccatcatc gccatactct ctccctggct gctgttcagc cccgaacagc
     2041 tcgcccagcc aggctttaag ttcaccgcca aatccctctc ctgggcagtt tctggttttt
     2101 ctaattcggt tatctggctc attttcgccg cctttatgtt tggcacaggc tatgaaaaaa
     2161 ccgggcttgg acgccgcatt gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt
     2221 ttctcggcta tgcggtgatg ttctccgagc tgatcctcgc acctgtaaca ccgtccaact
     2281 cggcgcgtgg tgcggggatt atctatccca tcatccgtaa cctgccaccg ctctatcaat
     2341 cacaaccaaa cgacagcagt tcgcgcagca ttggctcgta catcatgtgg atggggattg
     2401 ttgccgactg cgtgaccagc gccattttct tgacggcgat ggcaccaaac ttgctgttaa
     2461 ttggactgat gaaaagcgca tctcacgcca cgctgagttg gggcgactgg ttcctcggga
     2521 tgttgccgct cagtattttg ctggtgctgc tggttccctg gctggcttat gtgctgtacc
     2581 cgccggtact gaagtctggc gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa
     2641 tgggcccgct ctgttcgcgt gaaaaacgga tgctggggct gatggtaggc gcgctggtgc
     2701 tgtggatttt cggcggtgat tatatcgatg ccgcgatggt cggttacagc gtggtggcac
     2761 tgatgctgct tctgcgcatt atcagttggg acgacattgt cagtaataaa gcggcgtgga
     2821 acgttttctt ctggctggcc tcgcttatca ccctcgctac cggactcaac aacactggtt
     2881 ttattagctg gtttggcaaa ctgttagcag gcagcttaag cggttattcg ccaacgatgg
     2941 tgatggtggc gttgattgtg gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg
     3001 cgtatacctc cgccctcgca ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc
     3061 cgctgccggt gttctgcctg atggttggcg cggcaattgg tctggggagc attcttacac
     3121 catacgccac cggacccagt ccgatttact acggtagtgg ttatctgcca acggcggatt
     3181 actggcgact gggggcgatt tttgggctga tattcctcgt gttgctggtg attaccggct
     3241 tactgtggat gcccgtggtg ttgctttaag tgaggacggt gccggaaatt gctctccggc
     3301 acccaatagt tttacgcagc cggtaactcc gccagcggcc agcgcggacg cacgctaacg
     3361 ccgagatccg ccgtcgcgcc tgctttaaac cgcaccattc cggcataggc gatcatcgcg
     3421 ccgttatcag tacaaaactc cggacgcgcg tagaacactt cgccgcggcg ttttttcatc
     3481 atttccgcca gcttcgcccg taacgtgcgg ttagcactca cgccgcccgc catgaccagt
     3541 cgcttaaagc ccgtctgatc caacgctcgc ttacacttaa tcatcaacgt atcgaccacc
     3601 gcatcttcaa aggcccgggc gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga
     3661 atcgtgttcg ccgcaaaggt tttcagacca gaaaagctga aatccagccc cggacggtcg
     3721 gtcatcggac gcgggaaaac aaagcgcccg gcagtaccct gagccgccat tttcgacagt
     3781 aacggtccgc caggataatc cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg
     3841 gcggcatcat cgatagactc gccgagcagc tcgtactgac caatgccagt cacgctgatt
     3901 aactgcgtat gaccgccgga caccagcagc gcgacaaacg gaaattcagg cgggttatct
     3961 tccagcatcg gcgctaacag atgcccttcc atatggtgta ccgggattgc cggaacgttc
     4021 caggcaaacg ccagagaacg ccccacggtt gcgccaacca gcagtgcgcc gactaatcca
     4081 gggcctgcgg tataggccac agcatcaatg tcttttgccg ttaagccaga ctcctttagc
     4141 gccgcctgga tcaacggtac ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg
     4201 acgccgccgt agtcagcgtg caatttcacc tgactataca attggttggc taacaaacct
     4261 ttttcatcgt cgtaaatggc gatgccggtt tcatcgcagg aagtttcaat acccagtaca
     4321 cgcatgactt gttttacctc gctttattac cgcgcagtgt aggaccaatg cgggttgatg
     4381 taaaactttg ttcgcccctg gagaaagcct cgtgtatact cctcaccctt ataaaaatcc
     4441 ctttcaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca
     4501 ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca
     4561 catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa
     4621 gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa
     4681 accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact
     4741 ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg
     4801 atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg
     4861 tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat
     4921 gatctgctgg cacgcactga cattgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag
     4981 cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc
     5041 gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc
     5101 gacttcctga tgacctacga caagcttgag ttcgtcgaaa cggtcgaaga gctggcagca
     5161 atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat
     5221 cagaggcaaa cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta
     5281 caacaacctg ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag
     5341 gttatcgctc gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg
     5401 tttggcggca atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac
     5461 gatcagggac gcagttacga tcgtttccgc gagcgggtga tgttccccat tcgcgataaa
     5521 cgcggtcggg tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg
     5581 aactcgccgg aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg
     5641 cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg
     5701 gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc
     5761 gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac
     5821 cgtgcaggcc gtgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac
     5881 ggccgtcagc tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga
     5941 aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt
     6001 ctgtttaaca gcctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg
     6061 agtacgctgg cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt
     6121 cgtcaggaat taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg
     6181 ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg
     6241 cgtatactta tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt
     6301 gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact
     6361 tgtctctccc agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat
     6421 aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct
     6481 gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag
     6541 gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc
     6601 tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga
     6661 tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt
     6721 aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg
     6781 tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc
     6841 cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg
     6901 ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca
     6961 tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc
     7021 tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt
     7081 ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa
     7141 tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag
     7201 acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc
     7261 tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct
     7321 ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc
     7381 tttcccagga agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca
     7441 gcgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac
     7501 gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg
     7561 ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc
     7621 agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac
     7681 gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc
     7741 tgtttaccgg caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc
     7801 cgtggtcgga aaaactgcac gatgtctctg aagaagtgca tcgcgccctg cagaaactgc
     7861 agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta
     7921 tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac
     7981 gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga
     8041 ttcaggaagg caacatcggt ctgatgaaag cggttgataa attcgaatac cgccgtggtt
     8101 acaagttctc cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg
     8161 atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc
     8221 gtatttctcg ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg
     8281 aacgtatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa
     8341 tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg
     8401 ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc
     8461 acgacgtgct ggctggcctg accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta
     8521 tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg
     8581 aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg
     8641 aagtgttgcg tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac
     8701 ccggcactag tccctctgca caaacgccac cttt
//
LOCUS       DAJTNA010000153         8661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTNA010000153 DAJTNA010000000
VERSION     DAJTNA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.155"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            63..839
                     /gene="eutC"
                     /locus_tag="OO161_003165"
     CDS             63..839
                     /gene="eutC"
                     /locus_tag="OO161_003165"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HCR5464138.1"
                     /translation="MTSESCALDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTG
                     RAGPRPRTQALLRFLADHSRSKDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDM
                     GRRLCAEAVEALKAQCVANPDVQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVG
                     TPFFVRYGRVKIEDQIGEILGAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEAD
                     RTCISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINMTR"
     gene            849..1508
                     /gene="eutL"
                     /locus_tag="OO161_003166"
     CDS             849..1508
                     /gene="eutL"
                     /locus_tag="OO161_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5464139.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1521..2021
                     /gene="eutK"
                     /locus_tag="OO161_003167"
     CDS             1521..2021
                     /gene="eutK"
                     /locus_tag="OO161_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5464140.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKESGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            2067..3119
                     /gene="eutR"
                     /locus_tag="OO161_003168"
     CDS             2067..3119
                     /gene="eutR"
                     /locus_tag="OO161_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR5464141.1"
                     /translation="MKKTRTANLHHLYHEPFPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLGLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(3125..4024)
                     /gene="hemF"
                     /locus_tag="OO161_003169"
     CDS             complement(3125..4024)
                     /gene="hemF"
                     /locus_tag="OO161_003169"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5464142.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(4028..4897)
                     /gene="amiA"
                     /locus_tag="OO161_003170"
     CDS             complement(4028..4897)
                     /gene="amiA"
                     /locus_tag="OO161_003170"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5464143.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            5111..5536
                     /locus_tag="OO161_003171"
     CDS             5111..5536
                     /locus_tag="OO161_003171"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5464144.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5523..5972
                     /locus_tag="OO161_003172"
     CDS             5523..5972
                     /locus_tag="OO161_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5464145.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6179..6754
                     /locus_tag="OO161_003173"
     CDS             6179..6754
                     /locus_tag="OO161_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5464146.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6850..7749
                     /gene="yfeX"
                     /locus_tag="OO161_003174"
     CDS             6850..7749
                     /gene="yfeX"
                     /locus_tag="OO161_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5464147.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSPDKLMAL"
     gene            complement(7807..8661)
                     /gene="pbp4b"
                     /locus_tag="OO161_003175"
                     /pseudo
     CDS             complement(7807..8661)
                     /gene="pbp4b"
                     /locus_tag="OO161_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
BASE COUNT         1981 a         2192 c         2440 g         2048 t
ORIGIN      
        1 ccggacaaac ggcccccgcg ctgtcagaag caaagtgcac caccaccacc tgtgcggcac
       61 cggtgacctc ggaaagctgc gcgctggatt taggttctgc ggaagcaaaa gcgtggattg
      121 gtgttgaaaa cccgcatcgc gcagacgtat taacagaact gcgtcgcagc accgtggccc
      181 gcgtttgtac cggtcgtgcc ggtccgcgtc cgcgtaccca ggcgttgctg cgtttcctgg
      241 ccgatcactc tcgttcgaaa gataccgtac tgaaagaagt gccggaagag tgggtgaaag
      301 cgcagggctt gctggaagta cgctccgaaa ttagcgacaa aaatctctac ctgactcgcc
      361 cggatatggg ccgccgcctg tgtgcagaag ctgttgaagc gctgaaagca cagtgtgttg
      421 ctaatccgga cgtacaggtc gttatttctg atggcctgtc aactgatgcc atcaccgtga
      481 actacgaaga gatcctgccg ccgctgatgg cgggcctgaa acaggccggg ctgaaagtcg
      541 gcacgccatt ctttgttcgt tatggtcgcg tgaagattga agatcagatc ggtgagatcc
      601 tcggcgcgaa agtggtgatc ctgctggtgg gggaacgtcc ggggttgggt cagtcagaaa
      661 gcctctcctg ttacgccgtt tactcgccgc gtatggcgac caccgttgag gccgatcgca
      721 cctgcatctc taacattcac cagggcggca cgccgccggt tgaagccgca gccgtcattg
      781 tggatttggc gaaacgtatg ctggagcaga aagcatccgg catcaacatg acccgataag
      841 gaggcatcat gccagcttta gatttgattc gaccgtcggt aaccgccatg cgggtgattg
      901 cctccgttaa cgctgaattt gcgcgtgaac taaaattgcc gccgcatatt cgtagcctcg
      961 ggctgatttc tgctgattcc gatgacgtca cctacattgc ggctgacgaa gcgaccaagc
     1021 aggcgatggt cgaagtggta tatggccgct cgctgtatgc gggagcagca cacggaccgt
     1081 caccgactgc cggtgaagtg ctgattatgc ttggtgggcc aaacccggcg gaagtgcgtg
     1141 ctggtctgga tgcgatggtt gcgcatattg aaaatggcgc tgctttccag tgggctaatg
     1201 acgcagaaaa caccgcattc ctggcacatg tagtttcgcg taccggttct tatctctcat
     1261 caaccgccgg gatcacgctt ggcgatccga tggcgtatct ggtggcaccg ccgctggaag
     1321 cgacctacgg cattgatgca gcgttgaaat ctgccgacgt acagctggtg acctatgtcc
     1381 cgccaccgtc tgaaaccaac tactcggcag catttttaac cgggagccag gccgcgtgta
     1441 aagcagcctg taacgccttt accgatgcag tgctggaaat cgcgcgtaat ccaatccagc
     1501 gtgcgtaacg gaggttgccg atgatcaatg cactgggatt gctggaagtg gacggcatgg
     1561 tcgccgcgat agatgcagcg gatgccatgc tcaaagcagc taacgttcgt ctgctcagtc
     1621 acgaagtgct tgaccctggt cgcttaacgc tggtggtgga aggcgatctg gcggcgtgtc
     1681 gtgcggcgct ggacgcaggt tgtgctgccg cgatgcgtac cgggcgtgtc atcagccgca
     1741 aggagagcgg tcggccagac gatgacaccc agtggctggt tactggcttt aaccgccagc
     1801 cgaagcaacc cgtaagggaa cccgacgcgc cagttatcgt cgcggaatct gctgacgagt
     1861 tgttggcgct gttaacatca gtacgtcagg gaatgacggc aggagaagtg gctgcccact
     1921 ttggctggcc gctggaaaaa gccagaaatg cgctcgaaca gctcttttct gccgggacgt
     1981 tacgtaaacg cagtagtcgt tatcgtctca agccccatta acctgtcgga ggtgccgggt
     2041 gtcctacaac acccggcatt aacatcatga aaaagacccg tacagccaat ttgcaccatc
     2101 tttatcatga acccttcccc gaaaacctga agctcacgcc gaaggtcgaa gtggataatg
     2161 ttcatcaacg acagacaacg gatgtctatg aacatgcttt aacgattacc gcctggcagc
     2221 agatttacga tcagctgcat ccgggcaagt ttcatggtga gtttacggaa attctactcg
     2281 atgatattca ggtttttcgt gaatacaccg gtctggcgct gcgtcagtcg tgcctggtct
     2341 ggccgaactc gttctggttt ggcattccgg cgacgcgcgg tgagcaggga tttatcggtt
     2401 cgcaatgtct gggaagcgcg gaaatcgcca cccgccctgg tggcactgaa tttgaactga
     2461 gcacgccgga tgattacacg atcctgggcg tggtgctttc tgaagatgtc atcacccggc
     2521 aggctaactt tttgcataac ccggatcggg tattacatat gctgcgtagc cagtcggcgc
     2581 tggaagtgaa agagcagcat aaagccgcgc tgtggggctt tgtccaacag gcgctggcga
     2641 cgttttgcga gaacccggaa aatctccatc agcctgcagt gcgaaaagtg ctgggggata
     2701 atttgctaat ggcgatgggg gcgatgctgg aagacgcgca accaatggtg acggcggaaa
     2761 gcatcagtca tcagagttac cgtagattac tttcccgcgc ccgtgaatat gtgctggaaa
     2821 atatgtctga gccggtgacg gtgctgggct tgtgtaatca actgcatgtc agtcgccgca
     2881 cgctacaaaa cgcgtttcac gctattttag gcattggccc aaacgcgtgg ctgaaacgca
     2941 ttcgcctgaa cgccgtacgc cgcgaactga taagcccgtg gtcgcaaagc acaacggtaa
     3001 aagacgccgc catgcagtgg ggattctggc atctggggca atttgtcacg gattaccagc
     3061 agctgtttgc cgagaagccg tcgttgacgt tgcatcagcg gatgcgggaa tgggggtgag
     3121 ggagttacac ccaatccctg accttaataa actcacttaa cgccgcttct gggctgccat
     3181 cttttggctg ataatcatac tcccagcgta ccagtggcgg cattgacatc aggatcgact
     3241 cggtgcgccc gccagtttgc aggccaaaca gagtgccgcg atcccagacc agattgaact
     3301 cgacataacg accgcgacgg tagagctgaa aattgcgctc gcgctcgccg taggccatcg
     3361 ctttacgtcg ctctacaatt ggtaaataag cgtcggtgta gcctttgcct accgcctgca
     3421 taaaggcaaa acagtggtcg aaatctggcg tgttcagatc atcaaagaac agcccgccaa
     3481 taccgcgctg ttcgttgcga tgtttgaggt agaagtaatc gtcgcaccac tttttgtaac
     3541 ggggataaac gtcttcacca aatggcaggc acaggtcacg ggcggtgcgg tgccagtgaa
     3601 tggcgtcttc ttcaaaacca tagaaagggg ttaaatcgaa gccgccgcca aaccaccaga
     3661 cgggctctgc acccggtttt tcggcaataa aaaaacgcac attcgcgtgg ctggtgggaa
     3721 catacgggtt atgcggatgc actaccagtg aaacgcccat cgcctcgaaa ctgcgcccgg
     3781 caagttccgg gcgatgagcg gtggcggaag caggcatcgc ctcgccgtgg acgtgagaaa
     3841 agttgacgcc tgcctgttcg aaaacaccac cattacgcaa cacccgacta cgcccgccgc
     3901 cgccagcttc gcgctgccaa ctatcttcga caaattctgc gccatcgacg gcggtcagct
     3961 gctgacaaat cgtatcctga aggttgagca gaaactgttt aacctggtgt gcgtcgggtt
     4021 tcataactta tcgctttttc gaatgtgctt tctggttgtc gaaccagtgg aaataactga
     4081 tcacgccttc agcaatcgct gtggcgattt tctgacgaaa cgccgccgtg cctaacagcc
     4141 gctcttcttc cgggttggtg ataaacgagg tttccaccag caccgaagga accgacggcg
     4201 atttcaacac cacaaacgcg gcttgttcgg tgttgcggct gtgcagttta tgcaccggct
     4261 taatcttttt cagaatatgc gagccaagcg tcaggctgtt tttaatagta tcggtttgca
     4321 ccagatcaaa cagcacttgc tgcaataggt gatccttgtc agtcgccttt ttaccggcaa
     4381 cttcatcggc gcggttttcg cgttcagaca ggtatttcgc cattgcgcta cttgccccac
     4441 ggttagagag ggcaaatacc gaagcaccgg cagctttcgg gttggtaaag ccatcggcat
     4501 gaattgacat aaacagatct gcgccatgtt tatgggcgat ttcaacgcga tcgtaaagtg
     4561 ggataaacgt atcgccagaa cgcgttaaac gcgcatcaat cccatgatta cgcaaaatgg
     4621 aacggacgtt tttagcaatc gccagcacca catgtttttc tttcgaaccg ttgcgtccga
     4681 tcgctccggt atcaattccg ccgtggcctg gatcgagaac aacgacacgt ttgccgccag
     4741 attttttggc tttcggcttg ctgtgtccgt tgctggtttt taaaggttcg tctttggcga
     4801 tggcttgcga cattcctgac aacgtcaggg cagccaaacc ggctttcagc acctggcggc
     4861 gcgaagtgag tgtttttagt ggtttaaaag tgctcatacg gcctgagttg taataataaa
     4921 gttccagatg ttatatcgta tcgcgtaatc cgttacgacg gtttgatgtt gagaattgtt
     4981 ttacttttca tttcaatagt tgacaactcc ccattatgcc actttttcaa ccgattttcg
     5041 tcagatattg gctaattcta ccgttcacgc cataatcagc cttttaaccc gcaaaaaggt
     5101 aacgaatacc atggagatac gcgtatttcg ccaggaagat ttcgaagagg tcatcaccct
     5161 ttgggagcgt tgcgacttgc tgcgtccgtg gaacgatccg gaaatggaca tcgagcgtaa
     5221 gatgaaccat gacgtcagtt tgtttttggt cgctgaggtg aacggtgaag tggtcggaac
     5281 ggtgatgggc ggttatgacg ggcatcgcgg gtctgcttat tatcttggcg tgcatccaga
     5341 gtttcgtggg cgtgggattg ccaatgcgtt gcttaatcgg ctggagaaaa agctgattgc
     5401 tcgtggctgc ccgaaaattc agatcaatgt gccggaagat aacgacatgg tgctcggaat
     5461 gtatgaacgc ctgggatatg aacacgccga catgctgagt ctgggtaagc gtttgattga
     5521 agatgaagag tactgagttc catcctgtcc attatgatgc gcacggacgc ctgcgtttac
     5581 ccttgctctt ctggcttgtg ctattgcttc aggcgcgaac ctgggtgctg tttatcattg
     5641 ccggtgcgtc gcgcgagcag ggtactgcgc tgctaaatct gttttatccc gatcatgata
     5701 atttctggct ggggttgatt cccggtattc cggcggtgct ggcgtttttg ttgagcggtc
     5761 ggcgggcttc gtttcctcgc acctggcatg tgctctattt tctgttgctg ttggcgcagg
     5821 tggttttact ttgctggcaa ccgtggctgt ggctgaacgg tgaatccgtt agcggtatcg
     5881 gtctggcgct ggtagtggcg gatattgtgg cgttaatctg gctgctgacc aatcgacgtt
     5941 tacgcgcttg ttttaatgaa gaaaaagaat aacgcagagg tgcgctttgt ttatgccgga
     6001 tgcggcgtga acgccttatc cggcctacaa aacatcgaaa attcaatata ttgcaggaga
     6061 aagtaggcct gataagcgta gcgcatcagg caattttgtg tttgtcatca acctttaacg
     6121 gcactttttg gtgaatttgc actccaagca acgttattga ataaccaaag gaagtgacat
     6181 gaaatcgctg cgtttaatgt tatgcgctat gccgttgatg ctgaccggct gttccacgat
     6241 gtcgtcagtt aactggtcgg ccgctaaccc gtggaactgg tttggatcgt ccaccaaagt
     6301 gagcgagcag ggcgtgggtg aattaacggc gtccacacca ctgcaagaac aagccattgc
     6361 tgatgcgctt gatggcgatt atcgcctgcg cagcggaatg aaaaccgcga acggcaacgt
     6421 ggtgcgcttt tttgaagtaa tgaaaggcga caacgtggcg atggtgatta acggcgatca
     6481 aggcacgatc agtcgcattg acgtgctgga tagcgatatt cctgctgaca ccggtgttaa
     6541 aatcggtaca ccgtttagcg acctttacag caaagcattt ggcaattgcc aaaaagctga
     6601 tggtgatgat aatcgtgccg tcgaatgtaa agccgaaggc agtcaacata ttagctacca
     6661 gttcagtggg gaatggcgtg gtcctgaagg gttaatgcct tcggacgata ccctgaaaaa
     6721 ctggaaagtc agtaaaatta tctggcggcg ttaatttgcg tctgaacaaa ccgccgccgc
     6781 gaaaaaacac gtaaaatagc gctcaacaat gccacggatt gcgtggcatt cttattttca
     6841 ggaggaacaa tgtctcaggt tcagagtggc attttgccag aacattgccg cgcggcgatt
     6901 tggatcgaag ccaacgtgaa aggggaagtt gacgccctgc gtgcggccag taaaacattt
     6961 gccgacaaac tggcaacttt tgaagcgaaa ttcccggacg cgcatcttgg tgcggtggtt
     7021 gcctttggta acaacacctg gcgcgctctg agcggcggcg ttggggcaga agagctgaaa
     7081 gattttccgg gctacggtaa aggccttgcg ccgacgaccc agttcgatgt gttgatccac
     7141 attctttctc tgcgtcacga cgtaaacttc tctgtcgctc aggcggcgat ggaagccttt
     7201 ggtgactgca ttgaagtgaa agaagagatc cacggcttcc gttgggtgga agagcgtgac
     7261 ctgagcggct ttgttgacgg tacagaaaac ccggcgggtg aagagacgcg ccgcgaagtg
     7321 gcggttatca aagacggcgt ggatgccggc ggcagctatg tgtttgtgca gcgttgggag
     7381 cacaacctga agcagctcaa ccggatgagc gttcacgatc aggagatgat gatcgggcgc
     7441 accaaagagg ccaacgaaga gattgacggc gacgaacgtc cggaaacctc tcacctcacc
     7501 cgcgttgatc tgaaagaaga tggcaaaggg ctgaagattg ttcgccagag cctgccgtac
     7561 ggcaccgcca gtggcactca cggtctgtac ttctgcgcct actgcgcgcg tctgcataac
     7621 attgagcagc aactgctgag catgtttggc gataccgatg gtaagcgtga tgcgatgttg
     7681 cgtttcacca aaccggtaac cggcggctat tacttcgcgc cgtcgccgga caagctgatg
     7741 gcgctgtaag cgtttctgat tctatgatta aaggccagcc agtaacaaaa ctgactggcc
     7801 tttttattac ttttgcttca acgccgcata cacctgatca actacccaac cataagttgc
     7861 aatcggtaac tgaccgcttt cgaacatatt gggatttttt tgtggatcgg caactggcga
     7921 atggggcttg ttgcttaaca tcacaatcgc catatgattg accggatcga taacggttac
     7981 tgtcccggtc cagccagtgt gaccgtaggt ttgcgggctt gccagcgtgc caaacgtcgg
     8041 cgtcatggtg gcattaccat tcacgcgcca gccgaggcca aaagtggcat cttccttaga
     8101 gctggtggtg aacatcttca ccgtttccgc actgaacagc tgcacatcgc catagccccc
     8161 gccgttcagc atcgtttgca ttaacaccgc aatatcgccg gtattggaaa acaaacctgc
     8221 gtgcccggaa acgccgccca tcgaataaaa ggctttttca tcgtgcacct gaccccagag
     8281 agtggaggtg cggatattcg ggaaatggat cacgccatcg cgggtattgc cgttcaattc
     8341 cgtggcggca atttgttgcg gtttaaagcc tttcagcagc gggttaaata ccgtatgtgt
     8401 caggccgagc gggcgataaa tcgactcttc aacatagcgg tcaagcggct gaccggtaac
     8461 cgactcgacg ataaatccaa gcagcatata atcgacatcg ctgtagatat gtttgctgcc
     8521 gggctgatat tccagcggcg tgcgcttgat catctccagc gtctggcctt tatcctagga
     8581 atataacgcg cccgcgaccg ctttattcgg gtattgcgga tctgccggga aaccgccgct
     8641 gtgatgcagc aggtcagaaa t
//
LOCUS       DAJTNA010000154         8631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTNA010000154 DAJTNA010000000
VERSION     DAJTNA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.156"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            615..2543
                     /gene="thrS"
                     /locus_tag="OO161_003176"
     CDS             615..2543
                     /gene="thrS"
                     /locus_tag="OO161_003176"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5464148.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2547..3089
                     /gene="infC"
                     /locus_tag="OO161_003177"
     CDS             2547..3089
                     /gene="infC"
                     /locus_tag="OO161_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5464149.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3186..3383
                     /gene="rpmI"
                     /locus_tag="OO161_003178"
     CDS             3186..3383
                     /gene="rpmI"
                     /locus_tag="OO161_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5464150.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3436..3792
                     /gene="rplT"
                     /locus_tag="OO161_003179"
     CDS             3436..3792
                     /gene="rplT"
                     /locus_tag="OO161_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5464151.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3909..4033
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3915..3959
                     /gene="pheM"
                     /locus_tag="OO161_003180"
     CDS             3915..3959
                     /gene="pheM"
                     /locus_tag="OO161_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5464152.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4242..5225
                     /gene="pheS"
                     /locus_tag="OO161_003181"
     CDS             4242..5225
                     /gene="pheS"
                     /locus_tag="OO161_003181"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5464153.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDSFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5240..7627
                     /gene="pheT"
                     /locus_tag="OO161_003182"
     CDS             5240..7627
                     /gene="pheT"
                     /locus_tag="OO161_003182"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5464154.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKALLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWSGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7632..7931
                     /gene="ihfA"
                     /locus_tag="OO161_003183"
     CDS             7632..7931
                     /gene="ihfA"
                     /locus_tag="OO161_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5464155.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(8235..8375)
                     /locus_tag="OO161_003184"
     CDS             complement(8235..8375)
                     /locus_tag="OO161_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR5464156.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2210 a         2039 c         2276 g         2106 t
ORIGIN      
        1 aagctctgat gaaggcgttg gtatgtcgct atcgatgtgc aatctatgag gagacttata
       61 atgagttctg ataatgatat cgttttgact cataatctat tcaccttata tatactggag
      121 gttttgagga tgatgattaa ttattatcgc cagaacgaaa tgtgcacgaa aaattgctat
      181 aagaattgtg tcacttttat cattgtgata tgaccacttc gtcattaatc tcatgatcgt
      241 ttaaatacta ccgccaaatc acgcaacgaa ctttgctgga ttcagtttat gctgctgtaa
      301 atccgctcgc agtaaacctt tcagacgcac cgtgatgtta tcagtggttc tttaagcgtt
      361 ttgctggtgt actcactaca aacgaattgc gaatcgaatc aatgtgaaac ggaaaggtac
      421 aatctccctt tttttagtgt taacatcgtt caaccgggtg gaaggtgaga ggttaatgtc
      481 tttcatttgt gtacctgatt gcgaaccaat ttagcatttg ttggctagat ggtttcgcaa
      541 tgaactgtta ataaacaaat ttttctttgt atgtgatctt tcgtgtgggt caccactgca
      601 aataaggata taaaatgcct gttataactc ttcctgatgg cagccaacgc cattacgacc
      661 acgctgtaag ccccatggat gttgcgctgg acattggtcc aggtctggcg aaagcctgta
      721 tcgcagggcg cgttaatggc gaactggttg atgcttgcga tctgattgaa aacgacgcac
      781 aactgtcgat cattaccgcc aaagacgaag aaggtctgga gatcattcgt cactcctgtg
      841 cgcacctgtt agggcatgcg attaaacaac tttggccgca taccaaaatg gcaatcggcc
      901 cggttattga caacggtttt tattacgacg ttgatcttga ccgcacgtta acccaggaag
      961 atgtcgaagc actcgagaag cggatgcatg agcttgctga gaaaaactac gacgtcatta
     1021 agaagaaagt cagctggcac gcagcgcgtg aaactttcgc caaccgtggg gagagctaca
     1081 aagtctccat tcttgacgaa aacatcgccc atgatgacaa gccaggtctg tacttccatg
     1141 aagaatatgt cgatatgtgc cgcggtccgc acgtaccgaa catgcgtttc tgccatcatt
     1201 tcaaactaat gaaaacggca ggggcttact ggcgtggcga cagcaacaac aaaatgttgc
     1261 aacgtattta cggtacggcg tgggcagaca aaaaagcact taacgcttac ctgcagcgcc
     1321 tggaagaagc cgcgaaacgc gaccaccgta aaatcggtaa acagctcgac ctgtaccata
     1381 tgcaggaaga agcgccgggt atggtattct ggcacaacga cggctggacc atcttccgtg
     1441 aactggaagt gtttgttcgt tctaaactga aagagtacca gtatcaggaa gttaaaggtc
     1501 cgttcatgat ggaccgtgtc ctgtgggaaa aaaccggtca ctgggacaac tacaaagatg
     1561 caatgttcac cacatcttct gagaaccgtg aatactgcat taagccgatg aactgcccgg
     1621 gtcacgtaca aattttcaac caggggctga agtcttatcg cgatctgccg ctgcgtatgg
     1681 ccgagtttgg tagctgccac cgtaacgagc cgtcaggttc gctgcatggc ctgatgcgcg
     1741 tgcgtggatt tacccaagat gacgcgcata tcttctgtac tgaagaacaa attcgcgatg
     1801 aagttaacgg atgtatccgt ttagtctatg atatgtacag cacttttggc ttcgagaaga
     1861 tcgtcgtcaa actctccact cgtcctgaaa aacgtattgg cagcgacgaa atgtgggatc
     1921 gtgctgaggc ggacctggcg gttgcgctgg aagaaaacaa catcccgttt gaatatcaac
     1981 tgggtgaagg cgctttctac ggtccgaaaa ttgaatttac cctgtatgac tgcctcgatc
     2041 gtgcatggca gtgcggtaca gtacagctgg acttctcctt gccgtctcgt ctgagcgcct
     2101 cctatgtggg cgaagacaac gagcgtaagg taccggtaat gattcaccgc gcaattctgg
     2161 ggtcgatgga acgtttcatc ggtatcctga ccgaagaatt cgctggtttc ttcccgacct
     2221 ggcttgcgcc agttcaggtt gttatcatga atattaccga ttcacagtct gaatacgtta
     2281 acgaattgac gcaaaaacta tcaaatgcgg gcattcgtgt taaagcagac ttgagaaatg
     2341 aaaagattgg ctttaaaatc cgcgagcaca ctttgcgtcg cgtcccatat atgctggtct
     2401 gtggtgataa agaggtggaa tcaggcaaag ttgccgttcg cacccgccgt ggtaaagacc
     2461 tgggaagcat ggacgtaaat gaagtgatcg agaagttgca acaagagatt cgcagccgca
     2521 gtcttaaaca attggaggaa taaggtatta aaggcggaaa acgagttcaa acggcgcgcc
     2581 ctaaccgtat caatggcgaa attcgcgccc aggaagttcg cttaacaggt ctggaaggcg
     2641 agcagcttgg tattgtgagt ctgagagaag ctctggagaa agcagaagaa gccggagtag
     2701 acttagtcga gatcagccct aacgccgagc cgccggtttg tcgtataatg gattacggca
     2761 aattcctcta tgaaaagagc aagtcttcta aggaacagaa gaaaaagcaa aaagttatcc
     2821 aggttaagga aattaaattc cgtcctagta cagatgaagg cgactatcag gtaaaactcc
     2881 gcagcctgat tcgctttctc gaagagggtg ataaagccaa aatcacgctg cgtttccgcg
     2941 gtcgtgagat ggcgcaccag caaatcggta tggaagtgct taatcgcgtg aaagacgatt
     3001 tgcaagaact ggcagtggtc gaatccttcc caacgaagat cgaaggccgc cagatgatca
     3061 tggtgctcgc tcctaagaag aaacagtaag gccttcaagt agcactgtct gtggagcctt
     3121 cgggcttcac aggctttgtt cgcctttgtt tcgtttatta acaatgcgaa gtggaagtta
     3181 ttaaaatgcc aaaaattaag accgtacgcg gtgctgctaa gcgcttcaaa aaaaccggta
     3241 aaggtggttt taagcacaag cacgctaacc tgcgtcacat tctgaccaaa aaagcgacca
     3301 aacgtaaacg tcacctgcgt ccgaaagcca tggtttccaa aggcgatctg ggcctggtaa
     3361 tcgcgtgcct gccgtacgca taagccgtta acgtttttaa ctttttaatt agaatataga
     3421 tacaggagag cacatatggc tcgcgtaaaa cgtggtgtta ttgcacgcgc acgtcacaag
     3481 aaaattttga aacaagctaa aggctactac ggtgcgcgtt ctcgcgtata ccgcgttgcc
     3541 ttccaggctg ttatcaaagc tggtcagtat gcttaccgtg accgtcgtca acgtaagcgt
     3601 cagttccgtc aactgtggat tgcacgtatc aacgcagcag cacgtcagaa cggtatttct
     3661 tacagcaaat tcatcaatgg cctgaaaaaa gcctctgttg aaatcgaccg taagatcctg
     3721 gctgatatcg cagtattcga caaagtagcg ttcaccgctc tggttgaaaa agcgaaagca
     3781 gctctggcat aagccagttg aaagagggag ctagtctccc tcttttcgtt tcaacgccat
     3841 caaaacattg acttttatcg ccgtagcctt ttcaataaag gtcttttgaa gagtaccaaa
     3901 aggtaacgca agcaatgaat gctgctattt tccgcttctt tttttacttt agcacctgaa
     3961 tccaggaggc tagcgcgtga gaagagaaac ggaaaacagc gcctgaaagc ctcccagtgg
     4021 aggctttttt tgtatgcgcg ttttgaaaaa taaaaagcca gtctatcagg cgtaattgtc
     4081 gcagccagtc tggatacggc cagcgcggag aaaccggagc gtatttaagt acgtgagaat
     4141 ttcgagcact gcccgggacc aaaatggcaa gtaaaatagc ctgatgggat aggctctaag
     4201 tccaacgaac cagtgtcacc actgacacaa tgaggaaaac catgtcacat ctcgcagaac
     4261 tggttgccag tgcgaaggcg gccattagcc aggcgtcaga tgttgccgcg ttagataatg
     4321 tgcgcgtcga atatttgggt aaaaaagggc acttaaccct tcagatgacg accctgcgtg
     4381 agctgccgcc agaagagcgt ccggcagctg gtgcggttat caacgaagcg aaagagcagg
     4441 ttcagcaggc gctgaatgcg cgtaaagcgg aactggaaag cgcagcgctg aatgcgcgtc
     4501 tggcggcgga aacgattgat gtctccctgc caggtcgtcg cattgaaaac ggcggtctgc
     4561 atccggttac ccgtaccatc gaccgtatcg aaagtttctt cggtgagctt ggctttaccg
     4621 tggcaaccgg gccggaaatc gaagacgatt atcataactt cgatgctctg aacattcctg
     4681 gtcaccaccc ggcgcgcgct gaccacgact ctttctggtt tgacactact cgcctgctgc
     4741 gtacccagac ctctggcgta cagatccgca ccatgaaagc ccagcagcca ccgattcgta
     4801 tcatcgcgcc tggccgcgtt tatcgtaacg actacgacca gactcacacg ccgatgttcc
     4861 atcagatgga aggtctgatt gttgatacca acatcagctt taccaacctg aaaggcactc
     4921 tgcacgattt cctgcgtaac ttctttgagg aagatttgca gattcgcttc cgtccttcct
     4981 acttcccgtt caccgagcct tctgcagaag ttgacgtcat gggtaaaaac ggtaaatggc
     5041 tggaagtgct gggttgcggg atggtgcatc cgaacgtgct gcgtaacgtt ggcatcgacc
     5101 cggaagttta ctctggtttc gccttcggga tggggatgga gcgtctgact atgttgcgtt
     5161 acggcgtcac cgacctgcgt tcattcttcg aaaacgatct gcgtttcctc aaacagttta
     5221 aataaggcag gaatagatta tgaaattcag tgaactgtgg ttacgcgaat gggtgaaccc
     5281 ggcgattgat agcgatgcgc tggcgaatca aatcactatg gcgggcctgg aagttgacgg
     5341 tgtagaaccg gttgccggta gcttccacgg cgtggtcgtt ggtgaagtgg ttgagtgtgc
     5401 gcagcatccg aacgctgaca aactgcgtgt gacaaaagtg aatgtcggcg gcgatcgcct
     5461 gctggacatc gtctgtggtg cgccaaactg ccgtcagggc ctgcgtgtgg cggtagctac
     5521 cattggtgct gttctgccgg gtgatttcaa aattaaagcg gcgaaactgc gtggcgaacc
     5581 gtctgaaggg atgctgtgct ccttctctga actgggaatt tctgacgatc acagcggcat
     5641 tatcgaactg cctgcagatg cgccgattgg cactgacatc cgcgaatacc tgaaactcga
     5701 tgacaacacc atcgaaatca gcgtaacgcc aaaccgtgcc gactgcttag gtatcattgg
     5761 tgttgcgcgt gacgttgccg tgctgaatca gctgccgctg gttgaaccgg aaatcgttcc
     5821 ggttggtgcg accatcgacg acacgttgcc gattacagtc gaagcgcctg aagcctgccc
     5881 gcgctatctt ggccgtgtgg taaaaggcat taacgttaaa gcgccaactc cgctgtggat
     5941 gaaagaaaaa ctgcgtcgtt gcgggatccg ttctatcgat gcagttgttg acgtcaccaa
     6001 ctatgtactg ctcgaactgg gccagccgat gcacgctttc gataaagatc gcattgaagg
     6061 cggcattgtg gtgcggatgg caaaagaggg cgaaacgctg gtgctgcttg atggcactga
     6121 agcgaagctg aatgctgaca ctctggtcat cgccgaccac aacaaggcgc tggcgatggg
     6181 cggcatcttt ggtggcgaac actctggcgt gaatgacgaa acgcaaaacg tcttgctgga
     6241 atgcgctttc ttcagcccgc tgtctatcac cggtcgtgct cgtcgtcatg gcctgcatac
     6301 tgatgcgtct caccgttatg agcgtggcgt tgatccggca ctgcagcaca aagcgatgga
     6361 acgtgcgacc cgtctgctga tcgacatctg cggtggtgag gctggtccgg taattgatat
     6421 caccaacgaa gcaacgctgc cgaagcgtgc aaccatcact ttacgtcgta gcaaactgga
     6481 ttgcctgatc ggccatcata ttgctgatga acaggtaact gacattctgc gtcgtctcgg
     6541 ctgcgaagtg accgaaggca aagacgagtg gcaggcagtt gcgccgagct ggcgtttcga
     6601 tatggagatt gaagaagatc tggtcgaaga agtcgcgcgt gtttacggct acaacaacat
     6661 cccggatgag ccggtacagg caagcctgat tatgggtact caccgtgaag ctgacctgtc
     6721 gctcaagcgc gtgaaagcgc tgctcaacga caaaggctat caggaagtga tcacctacag
     6781 cttcgttgat ccgaaagtgc agcagatgat ccatccaggc gttgaagcct tactgctgcc
     6841 aagcccgatc tctgttgaaa tgtcagcaat gcgtctttct ctgtggagcg gcctgctggc
     6901 aaccgtggtg tacaaccaga accgtcagca gaaccgtgtg cgcattttcg aaagcggtct
     6961 gcgtttcgta ccagatactc aggcaccgtt gggcattcgt caggatctga tgttagccgg
     7021 tgtgatttgc ggtaaccgtt acgaagagca ctggaacctg gcaaaagaga ccgttgattt
     7081 ctatgatttg aaaggcgatc ttgaatccgt tctcgacctg accggtaaac tgaatgaggt
     7141 tgagttccgt gcagaagcga atccggcact gcatccgggg caatccgcag cgatttatct
     7201 gaaaggggaa cgtattggtt ttgttggagt tgttcatcct gaactggaac gtaaactgga
     7261 tcttaacggt cgcactctgg tgttcgaact ggagtggaac aagctcgcag accgcgtggt
     7321 gcctcaggcg cgcgagattt ctcgcttccc ggcgaaccgt cgtgacatcg cggtggtggt
     7381 cgcagaaaac gttcccgcag cggatatttt atccgaatgt aagaaagttg gcgtaaatca
     7441 ggtagttggc gtaaacttat ttgacgtgta ccgcggtaag ggtgttgcgg aggggtataa
     7501 gagcctcgcc ataagcctga tcctgcaaga taccagccgt acactcgaag aagaggagat
     7561 tgccgctacc gtcgccaaat gtgtagaggc attaaaagag cgattccagg catcattgag
     7621 ggattgaacc tatggcgctt acaaaagctg aaatgtcaga atatctgttt gataagcttg
     7681 ggcttagcaa gcgggatgcc aaagaactgg ttgaactgtt tttcgaagag atccgtcgcg
     7741 ctctggaaaa cggcgaacag gtgaaactct ctggttttgg taacttcgat ctgcgtgata
     7801 agaatcaacg cccgggacgt aacccgaaaa cgggcgagga tattcccatt acagcacggc
     7861 gcgtggtgac cttcagaccc gggcagaagt taaaaagccg ggtcgaaaac gcttcgccca
     7921 aagacgagta atctgatcta actaaaaagg ccgctctgcg gccttttttc tttcaataac
     7981 taattgattt ataatgtttt atatctcttc gttccacgat gcgtccacat ttagtggggg
     8041 gaggggagta agcccgctac attgtgggct ttgtcttata gtctgatcac tgtttcaagt
     8101 cggtgaggac accaaaaaga aaccccgcag tttttacgct gcgggtttgt tgttcatgtc
     8161 tgtgagatag ggtgccttat cgaccttacc ctggcaaccg attgacgggg gattgctccc
     8221 ccgtcgcggt ttccttactg cttacactgt aagaacgccg caaactccgc tccccagaag
     8281 ctcatccgta tttcgcacag cgaaccgtgc agcatccaga tgatgaggat tgccgtcacg
     8341 cagaacgtga tggccgtaag cgatttttgc gacatagcgc ttgctccttt ttcggggagg
     8401 cgctaaccta tcacttgctt agggtagacg gttagggcct cggttaaaca aaaatgtttt
     8461 ccggggcctt tccacatctg gccttcaggt attccctccg accatcagcc gaaaggcacc
     8521 cgcgcgtaat ctatcgcttt tttgttactc cggcaattct gctcgttaat tctgaggtaa
     8581 gggcaaactc atctgattgt ttcttctgtg tgaagttggc agttcatgcc a
//
LOCUS       DAJTNA010000155         8626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTNA010000155 DAJTNA010000000
VERSION     DAJTNA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.157"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            76..1719
                     /gene="eptA"
                     /locus_tag="OO161_003185"
     CDS             76..1719
                     /gene="eptA"
                     /locus_tag="OO161_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5464157.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            1716..2384
                     /gene="basR"
                     /locus_tag="OO161_003186"
     CDS             1716..2384
                     /gene="basR"
                     /locus_tag="OO161_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5464158.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            2385..3485
                     /gene="pmrB"
                     /locus_tag="OO161_003187"
     CDS             2385..3485
                     /gene="pmrB"
                     /locus_tag="OO161_003187"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5464159.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRARVRLKKDQNVANQI"
     gene            complement(3461..3550)
                     /gene="pmrR"
                     /locus_tag="OO161_003188"
     CDS             complement(3461..3550)
                     /gene="pmrR"
                     /locus_tag="OO161_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5464160.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(3662..5164)
                     /gene="proP"
                     /locus_tag="OO161_003189"
     CDS             complement(3662..5164)
                     /gene="proP"
                     /locus_tag="OO161_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5464161.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(5429..6307)
                     /locus_tag="OO161_003190"
     CDS             complement(5429..6307)
                     /locus_tag="OO161_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5464162.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(6304..8532)
                     /gene="crfC"
                     /locus_tag="OO161_003191"
     CDS             complement(6304..8532)
                     /gene="crfC"
                     /locus_tag="OO161_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5464163.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAAQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSQPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTFCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTKLQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2203 a         2289 c         2126 g         2008 t
ORIGIN      
        1 caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc
       61 gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc
      121 tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag
      181 gtgttgcagg cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg
      241 gtcgtcgctt tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat
      301 cgaccactgg cctgcctgtt tattctggtt ggcgcggctg cacaatattt cataatgact
      361 tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccggcagaa
      421 agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct
      481 gcgctgattg cctgctggat aaaaatcaaa cctgccacct cgcgtctacg cagtgttctt
      541 ttccgtggag ccaatattct ggtttctgta ctactgattt tgctggtcgc cgcactgttt
      601 tataaagact acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc
      661 tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctaccg
      721 ctggtgcgaa ttggtgaaga cgcccaccgc aacccgttaa tgcagaacga aaaacgtaaa
      781 aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc
      841 tacccgcgtg aaactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc
      901 gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt
      961 gagcattaca aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga
     1021 gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc
     1081 gtgcctcacc agaacgtcac cgcgctgaat ctgcctggtc agtgcatcaa cggcgaatgc
     1141 tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc
     1201 gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg
     1261 cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag
     1321 caactggtga acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg
     1381 attaatctgc tgaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac
     1441 cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc
     1501 cctgatagcc aaaaacaggt gccgatgctg ctgtggctgt cggaggatta tcaaaaacgg
     1561 tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac
     1621 aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggctgcg
     1681 gatgatattc tgcaaacttg caggagagtg agtaaatgaa aattctgatt gttgaagacg
     1741 atacgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg
     1801 gcgtgacaac cgcgcggatg gcggaacaaa gccttgaagc cggtcattac agcctggtgg
     1861 tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga
     1921 aaaaatatac cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg
     1981 ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac
     2041 acgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg
     2101 ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga
     2161 ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg
     2221 tacatcggga aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca
     2281 ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg
     2341 tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt
     2401 ttctgcgccg accaatatcg ctgcgccaac ggctgatatt gactatcggg gccattttgt
     2461 tggtgtttga gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc
     2521 tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc
     2581 gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta
     2641 tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg
     2701 aagcgcgcac tgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg
     2761 aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg
     2821 aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt
     2881 tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc
     2941 ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat
     3001 tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg
     3061 acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg
     3121 ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag
     3181 aaaacgccca tcgttacagc ccgcaaggca gcaacattat gattaaactg caagaagatg
     3241 gcggagcggt catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg
     3301 agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa
     3361 gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag
     3421 agacttccgg cacgcgggcc agggtacggc tgaagaaaga tcagaacgtg gcaaaccaga
     3481 tataaagaaa gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac
     3541 ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag
     3601 tgaaccttaa gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag
     3661 cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg
     3721 caatctcgtg gtcaatatta tcgattttct gctcgatatt atcgtaatgc tcgacgagaa
     3781 tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg
     3841 ccgtctcttt catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat
     3901 aggcaggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca
     3961 gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg
     4021 tcgggaacat cgctggcaag gtagaagcca taacgcccgt aaagcagtta aggatcaccg
     4081 ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg
     4141 ccgggatcgc caacacaaac agggcaacac taccaagtag cacaaacgga cgacggccaa
     4201 aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga
     4261 taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca
     4321 tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca
     4381 acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca
     4441 aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt
     4501 ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa
     4561 tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca
     4621 cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc
     4681 cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccgc
     4741 cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa
     4801 tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag
     4861 tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc
     4921 caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc
     4981 tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac
     5041 caaaatcgaa ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt
     5101 tactgtcatt aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca
     5161 gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg
     5221 gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg
     5281 cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt
     5341 aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt
     5401 ttaggacatc attgatgtaa ctgattattt acgccaaatt tacagagaag atttcctcga
     5461 ccattgcccc caccaaacgc tgcgctgaac agagtcgtgg catccccagc gcgacagttt
     5521 gccaacgttg ggtcactgac cagcaaacgg gttgtttatc tgccgtggtc cactttccca
     5581 gccaacttaa aaaatcagca tgatccccgc ccgtcggaac ggtcgaagag gcaaaccact
     5641 gatggtaata gtgacgcgtg gtcggggccg cattgatact ctctgccaga tgttgtgtcg
     5701 ctttcacgcg caaatcaccc agcagcaacg ccaccgcttg cgggcttccc agacgacagg
     5761 catcgccaaa tgccccccaa cgcaactctt cgagaacgat aaaacagcgc ccaggcaacg
     5821 accaggcgtt atacgcccca gcacgccagc gggtaaaaat ttgctcgctc tgatcacctg
     5881 cctgcaccag taggcctcgt tgcgttaacg ccttttcctt ccagcgacaa cgggctgaaa
     5941 accactcata caaggcggca atttgctggg ggagctccgg gaaagcagag tgctggcggt
     6001 gatccatttt ttgcagcagc gccagatgct gcgcgatctg cgtcagcgcc agatgaccgg
     6061 gatccagcat cgacgccagt ttttccacca ttgtcagtcg caggctaagc ggcgcattta
     6121 acatgccgcc gttcgtccat gtctgaagct gcacacgcga aagaagttcc tgcatcagtc
     6181 gttcacgaaa ctgctgctga tgggcttgtg gaaggcgggc atcatcgccc tgcgccagat
     6241 ccactaaaaa gcggggataa accgactcca gcacgcgacc ggggccgtcc agtaacgtct
     6301 tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca tcgcgtaaca gctgggcgtc
     6361 ggtgtaaatc cagccgtgag tcatcacagt ctgctgcaat tgctgctgca tcagcctgac
     6421 caccgattca ttttgttgac gcagagccag gctttcgcgt aaacgcgtct gtaatttcgt
     6481 caaacatgaa gcgaactcag cgaaaaaagt attcatgcct gccgtaacag attcatcgac
     6541 ctgctctgcc agaactttag ccatttgttg gcaataaaga tcgacttctg cgcttaatgc
     6601 tcgttgcaac acactgtaat caaccgtttc tgtcggggat ttctcatttc cccgtcccca
     6661 gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca acgcctaaac gcgtgctctg
     6721 ctcgtccgtt gcctcctggc gcgaaatgac ttcactgaac agctggcgag tgttgacgtg
     6781 gggaactact ccgtgaaaaa caggaaaatg aaacccagga cgaaaccctg actcgctcaa
     6841 ttccattttg acttgttgct caatgggccg aataacatcg gttaacaccc ggcaaaaggt
     6901 ggattcaagc tcggcaaaac gcagcgtaaa gtcgcgactg atggtgttct gcgccgtctg
     6961 taacagtgtc tcacagcagg tacgaatctc gcttaacggc tgcgaatcat cctgaaacaa
     7021 ggcggctaac tgcgcattca gcgcatcttg ctgttgacgc agaaagtggt tggcggaggt
     7081 cagggccagc tcgatttcat gtttaatctc gccgctcacc tgcgcctgat tgagttgcaa
     7141 tagctgcaaa ctttcttcga cctgatggat attttgccgc aattgttcac aagcgacgtt
     7201 taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc tgcgcgtaat tcaacagttt
     7261 atgcgcagca gatcgcagag catacaatga ggcgttagcg taagcggcat gaagcaacgc
     7321 ctgaattggc tgggcgaaca gcgaatcttc ccacaactga tcggcagcat gacgaatatg
     7381 ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg agcgcggcat gggcaaaatc
     7441 ttccacccag cgttgttgct ctggcgctgg taacttaccg ttgttggcta actcatagcg
     7501 cgcccgattc gccaggtagc cccacatcga cgacaccgga aatatctgct gtggcgtaat
     7561 acagcctttc atcagcgtcc cggaaatcag tgcccgcacc tggtcggcgt cgtcactgtt
     7621 acgatcctgt tgatcgaact tattgaccag cacatacagc ggcaccgatt gccccaccgc
     7681 caaaatcgcc tcacggacct cttcatcaga gatcgatttc agttgcgtat aatccagcac
     7741 cgccagtacc gccgaggcgc gtgccagctg ctggttaagc attttttgca gatgcggttg
     7801 cccggcttca tttggcccgg gggtatccag taacgtcaac tgaccgggat aactttccag
     7861 ccccgccaga tggacaaact ccacttcaat cacgggaata tgctcaatgg cggcgtaagc
     7921 agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc actaaatcat tcaaactttt
     7981 cagacaatga aaaataggct gggcacccag ataatatttt tcgaaagcga cgccattttc
     8041 gatccgctgc ataagcgcac gcatatcttt atctatttcc agcacatcgg tcagatgctt
     8101 aatatcgcaa tcacgcaggc gctgttgtaa ttgttgaatt aaacaatcga ttggcgcgac
     8161 atgtgaaaaa tgcagtaccg gttccttttg cccgggcgta tggcgaataa gcgtcggcag
     8221 cgcagtcatt gggcgattac gattaggcag aacctccgta ccaacaatgg cattaatggt
     8281 ggttgatttc cctgctttca tagtaccgac aatcgccagc accatttcca gtcgggaaat
     8341 tttacgcaac tcattattca gcatcgcgta acgttcggcg atattaggct gactccaggg
     8401 taaagccagt tgtgccgcat cgtctccggg tacagagaga ggcatttttt ccagtaactg
     8461 caactgttgg cgagaaagct gtaacaggcg ttcagcctcc tgacttaact catacagggt
     8521 ctgtgtgtac atagaaaatt cttccttaaa gcaaattttg ttacttttat ttagccagat
     8581 tgtttttgag ttctgttttc ggcttttata attactgcaa gaaata
//
LOCUS       DAJTNA010000156         8601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTNA010000156 DAJTNA010000000
VERSION     DAJTNA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8601
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.158"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..183
                     /locus_tag="OO161_003192"
     CDS             <1..183
                     /locus_tag="OO161_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5464164.1"
                     /translation="EAIKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQ
                     KGEVVGVDDQCKEHKE"
     gene            402..644
                     /locus_tag="OO161_003193"
     CDS             402..644
                     /locus_tag="OO161_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464165.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="OO161_003194"
     CDS             complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="OO161_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5464166.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="OO161_003195"
     CDS             complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="OO161_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5464167.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2532..3434)
                     /gene="fdoH"
                     /locus_tag="OO161_003196"
     CDS             complement(2532..3434)
                     /gene="fdoH"
                     /locus_tag="OO161_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5464168.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="OO161_003197"
     CDS             complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="OO161_003197"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5910..5912),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5464169.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6691..7524
                     /gene="fdhD"
                     /locus_tag="OO161_003198"
     CDS             6691..7524
                     /gene="fdhD"
                     /locus_tag="OO161_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5464170.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7678..8601
                     /locus_tag="OO161_003199"
                     /pseudo
     CDS             7678..8601
                     /locus_tag="OO161_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT         2048 a         2181 c         2272 g         2100 t
ORIGIN      
        1 gaggcaatta aacggctgaa caagcttcgg ggctggcgta aggtttcacg atctgcaatt
       61 ttacgcgaag cggttgagca atatctggag cggcagcaat ttccggtgcg taaagcaaag
      121 ggaggcaggc aaaaggggga ggtagttggt gttgatgatc agtgtaagga gcataaggaa
      181 tgaaaaaaat ttttcattct tgaatataaa aaaacagatg cccttattct ggtattaata
      241 caaggctgtt ttacttgaac ttataataac tgcaactgtt acatcgtatc tggaaaacgc
      301 ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact
      361 tttctctcca tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta
      421 tagatatggg cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc
      481 tcgaggtgca gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc
      541 tgataaatca atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg
      601 aagatggtgt cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt
      661 caatcagatt gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc
      721 cggcctacaa agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc
      781 agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt
      841 tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa
      901 tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg
      961 aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg gaactgcggg
     1021 catagccttc ttgctccatt cgcgcgtcca gtaccagaga agcgaggtca tctgccacgg
     1081 cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc
     1141 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
     1201 tctgttcaca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc
     1261 agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
     1321 aaccacaaac cgggcaatat tgacgttgct cgccgtattc agcgcgggct ttgccgggga
     1381 tcagattggc catctgcgcc cagtagagcg acagtgcagc ccagataaac ggcgctttat
     1441 cgctgctgac ggatgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
     1501 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
     1561 tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
     1621 gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca
     1681 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
     1741 gcaggtaatc atccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt
     1801 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     1861 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     1921 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac
     1981 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     2041 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     2101 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     2161 cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     2221 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     2281 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     2341 aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat
     2401 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat
     2461 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt
     2521 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     2581 tccgcacggt tcggaccgac acccacgtag tggaagatac tggctgcgaa ggtagccgca
     2641 aagccaacag ctgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     2701 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac
     2761 ataacgtgtg taccaccgac gcctgccgga tcgtacaggc ccgcattgtc gtaaccgcgg
     2821 gttttcagct ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     2881 tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg
     2941 tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg
     3001 aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg
     3061 ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag acagcctgga
     3121 tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc
     3181 acttccgaga agcgcatcac cgtccacgat ttggcactta aatcattggg gttgtcgtac
     3241 accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga
     3301 caggctttac agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag
     3361 tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttga
     3421 gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta
     3481 aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaaagtatt ggcaataaag
     3541 ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct
     3601 ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag
     3661 ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc
     3721 gactccccga tttccacaaa ctgctctggc tgcaaaatcg cgttcaacag cgcgtgtttg
     3781 gtccagtagt ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg
     3841 gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagccgggtt cgagataacg
     3901 tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac
     3961 ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata
     4021 aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc
     4081 cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga
     4141 tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc
     4201 agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc
     4261 ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat
     4321 tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg
     4381 gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga cttcggctca
     4441 tgcggaatgg cgtagttcca ggtcatgttc agcacctgat ccgggttcgc gccaccctgt
     4501 tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcacc gtcagtcagc
     4561 gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg
     4621 attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc
     4681 ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg
     4741 ttcagcgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt
     4801 ttgttcggga atgaggcaac agggttaaaa ccctggcaga tatagccatt gaccttgccc
     4861 tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag
     4921 tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag
     4981 acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc
     5041 gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa
     5101 cctggcaggc tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg
     5161 cgcagggcgt taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc
     5221 gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg
     5281 aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac
     5341 gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc
     5401 tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag
     5461 ccgttttcgt ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca
     5521 tagccggtga acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg
     5581 gtataggctt cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg
     5641 cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtc agctaccgcc
     5701 gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg aattttggct
     5761 tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg
     5821 acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt
     5881 ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc
     5941 atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa
     6001 gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca
     6061 atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc
     6121 caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg
     6181 ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg
     6241 accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg
     6301 tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg
     6361 gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca
     6421 aaacctagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc
     6481 cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc
     6541 gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc
     6601 cagaattggc tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg
     6661 gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa
     6721 aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa
     6781 cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt
     6841 tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg
     6901 ctttccgagg ggattatcga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc
     6961 tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag
     7021 cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat
     7081 gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg
     7141 gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact
     7201 cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt
     7261 cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag
     7321 caaggtgcgg tactggtttc cagtcgcgcc agttatgaaa tggtgcaaaa gtcggcgatg
     7381 tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct
     7441 gagcgctgta atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc
     7501 catccgcagc gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg
     7561 acttattcat ttcgtgaatt ttattatttt tatttataaa taaaacgcgt gtagtggcgt
     7621 tttctttttt catttattat gttcactctc ttatctatat atttataagg caattaaatg
     7681 aaaaggaatt tattatcttc cgctattata gtcgccatca tggccctcgg tctgacgggt
     7741 tgtgatgata aaaaagccga aacagaaacg cccccgcctg ccaatagtca acctgccgca
     7801 ccagctcctg aagctcccgt tgcaaaagca gaagctaaac ctgaaacacc tgcgcaaccg
     7861 gtggtcgatg aacaagcggt tttcgacgaa aaaatggatg tctatatcaa gtgctataac
     7921 aagttacaga tccctgtaca gcgcagtctg gcgcgttatg ttgactggct gaaagatttt
     7981 aaacaggggc ctaccggtga agagcgtact gtttatggca tctacggcat tagtgaatcc
     8041 aacctcgctg agtgtgaaaa aggcgtaaaa agtgctgtgg cgttaacgcc tgcgctgcaa
     8101 ccaattgatg gcgtggcggt gagttatatc gatgctgccg tggcgttggg taataccatt
     8161 aacgaaatgg ataaatatta cacccaggaa aattataaag acgatgcgtt tgcgaaaggg
     8221 aaaacgctgc accagacatt cttaaaaaat ctgtaagcct ttgaacctgt agcagaatct
     8281 tatcatgcgg cgattcagga aattaacgat aagcgccagc ttgcggaact gaaaaatatt
     8341 gaagaaagag aagggaaaat attccactac tattctctgg cagtcatgat ttcagcgaaa
     8401 caaattaata acctgatatc gcaagagaag tttgatgttg atgcggcaat gaagaaagtg
     8461 tctgaactgg aaacgctggt ggcgcaggcc aaagaagcgg ataaaggcgg catgaatttc
     8521 tcgtttatta attcggcagg ccagtatcaa ctcgaggcta aaaaatacgt tcgccgcatc
     8581 agagataaag tcccgtactc t
//
LOCUS       DAJTNA010000157         8549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTNA010000157 DAJTNA010000000
VERSION     DAJTNA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.159"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..291
                     /gene="csgC"
                     /locus_tag="OO161_003200"
     CDS             <1..291
                     /gene="csgC"
                     /locus_tag="OO161_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5464171.1"
                     /translation="GVSVKLRLPQYTIIPEVTLTQSCLCRVQILSLREGGSGQSQTKQ
                     EKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQWPPSSEKS"
     gene            412..723
                     /locus_tag="OO161_003201"
     CDS             412..723
                     /locus_tag="OO161_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5464172.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEY
                     N"
     gene            819..1352
                     /gene="ymdB"
                     /locus_tag="OO161_003202"
     CDS             819..1352
                     /gene="ymdB"
                     /locus_tag="OO161_003202"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5464173.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1294..2775
                     /gene="clsC"
                     /locus_tag="OO161_003203"
     CDS             1294..2775
                     /gene="clsC"
                     /locus_tag="OO161_003203"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5464174.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPCSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2783..3940)
                     /gene="mdoC"
                     /locus_tag="OO161_003204"
     CDS             complement(2783..3940)
                     /gene="mdoC"
                     /locus_tag="OO161_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5464175.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4334..5869
                     /gene="mdoG"
                     /locus_tag="OO161_003205"
     CDS             4334..5869
                     /gene="mdoG"
                     /locus_tag="OO161_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5464176.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5862..8405
                     /gene="mdoH"
                     /locus_tag="OO161_003206"
     CDS             5862..8405
                     /gene="mdoH"
                     /locus_tag="OO161_003206"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5464177.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2157 a         2026 c         2227 g         2139 t
ORIGIN      
        1 ggagtgtccg ttaaactcag gctacctcag tataccatta ttcctgaagt cactcttact
       61 caatcttgtc tgtgcagagt acaaatattg tccctgcgcg aaggcggttc agggcaaagt
      121 cagacgaagc aagaaaagac cctctcattg cctgctaatc aacctattgc tttgacgaag
      181 ttgagtttaa atatttcccc ggaagatcgg gtgaaaatag ttgttactgt ttctgatgga
      241 caggcacttc atttatcaca acaatggccg ccctcttcag aaaagtctta atttgttgaa
      301 atgtcgagca taagatgaat ctggagagaa tggtctgctg cgaatcagcc aacctgaaag
      361 tatggataac acaaccctca aggatgacta atcattgagg aaatagaata aatgttcaga
      421 ccttttttaa actctcttat gctcggcagt ttgttttttc cttttattgc catcgcagga
      481 agcaccgcgc aagggggcgt gatccatttt tatggccaaa ttgtggaacc ggcatgtgac
      541 gtcagcaccc agtcatcacc cgtagaaatg aactgcccac agaatggttc tgttccggga
      601 aaaacctatt ccagcaaagc gttaatgagc ggcaatgtca aaaacgcgca agtagcctca
      661 gtaaaagtgc agtatattga taaacagaaa aagctggctg taatgaacat cgaatataac
      721 taagtttctg aacaacgcac gttgctgagt agaaaaatgc gatttaacca aaaagcctgc
      781 tgtacactta agaaacaaga aggtgttaag gaggcgttat gaaaacgcgt attcatgttg
      841 tgcagggtga tattaccaaa ctggccgttg atgtgattgt gaatgcggct aatccgtcat
      901 taatgggagg cggcggcgtc gatggggcca ttcatcgcgc agcgggtccg gccctgctgg
      961 atgcctgttt aaaagtcagg caacagcagg gcgattgccc tacggggcat gccgttatta
     1021 cgcttgcagg cgatcttccc gctaaagccg tagtacacac cgtcgggcca gtctggcgtg
     1081 gtggtgaaca aaacgaagac cagcttttgc aggatgccta tctcaatagt ctgcgactgg
     1141 tggcggcaaa cagctatacg tcagtggcat ttcctgcaat cagtactggg gtttatggtt
     1201 accctcgtgc ggcagcggct gaaatcgcag taaaaaccgt ttcagaattt attacccgtc
     1261 acgctttacc cgaacaggta tactttgtct gttatgatga agaaaacgcc cacctctacg
     1321 aaagactcct tacccaacaa ggagatgaat gatttgcccc ggctggcgag cgcggtgctg
     1381 ccactgtgtt cgcaacatcc cggtcagtgt ggtcttttcc ctctggagaa aagtctggat
     1441 gcgtttgccg cccggtatcg tctggccgaa atggcagagc atacgctgga tgttcagtat
     1501 tacatctggc aggacgatat gtcgggtcgg ttactgtttt ccgccctgtt agccgcagca
     1561 aagcgtggcg ttcgcgtccg tttgttgctg gacgacaaca atacgcccgg acttgacgat
     1621 attttacgct tgcttgacag ccatccccgc attgaagtcc ggctttttaa tcctttctcg
     1681 tttcgcttgc tgcgtccgct tggttatatc accgactttt cccgtctcaa tcgccgtatg
     1741 cacaataaaa gtttcactgt cgatggcgtg gttaccttgg tgggaggacg aaatattggt
     1801 gatgcctatt ttggggcagg ggaagagcca cttttttcgg atttagatgt tatggcaata
     1861 ggacccgtgg tagaggacgt tgccgatgat ttcgcccgct actggtattg caaatcggtt
     1921 tcacccttac agcaggtgct ggatgtcccg gaaggtgaaa tggcggatcg catcgagtta
     1981 cccgcctcct ggcataacga tgccatgacg catcgttact tacgccaaat ggaatccagt
     2041 ccgtttataa atcatctggt tgatggaacg ttgccgctta tctgggcgaa aacacgttta
     2101 ttaagtgatg atccggcgaa aggggagggc aaggcaaaac ggcattcact gttaccgcag
     2161 cgcctgttcg atatcatggg ctcacccagt gaacgcatcg atattatctc ttcctatttt
     2221 gtaccgacac gcgcaggtgt ggcgcaactc ttacggatgg tgagaaaagg ggtaaagatt
     2281 gcgatcctaa ccaattctct tgccgctaac gatgttgctg tcgtccatgc cggatacgcg
     2341 cgctggcgca aaaaattgct ccgctatggc gtggaattat atgaactcaa gccgacgcgt
     2401 gaacaaagta gtacgttaca cgatcgcggc ataaccggta attccggtgc cagcctgcat
     2461 gctaaaacct ttagcatcga tggtaaaacg gtgtttatcg gctctttcaa tttcgatcca
     2521 tgttcgacat tgctcaatac tgaaatgggc ttcgtgatag agagcgaaac gctggcacag
     2581 ttaattgata aacgctttat acagagccag tatgatgcgg cctggcagct ccgtctggac
     2641 aggtggggac ggatcaactg ggttgatcgt catgcaaaga aagagattat tctcaaaaaa
     2701 gaacccgcca ccagtttctg gaagcgggtt atggtcagac tggcgtcgat attgcccgtg
     2761 gaatggttat tgtaaatgtg gcttaacggg ctggtgcttt atcgttctca cgcttaacaa
     2821 ccggtttacc agaaaacaaa aacttcagta acgggatgcg taaatgaatt tcatacagaa
     2881 ttatcgcaat ccctactacg aatatcaggc cacagagaaa accaagccag ttggaggtga
     2941 tgtgcggtgt aatgtatgcg ccgaaaaaca gcgttaacgg gtggtgaacc agatagataa
     3001 atagcgatgc gttaacaaag taagtcaccc gcgctgactg gaagttaagc aaacggtggc
     3061 caaaggagaa gaccacattc accatccaca gaccgaggac catggtgatc accgactcgg
     3121 tttcgtacat ccaggcatcg ccactgccat agcgttggtt gagtaaataa gcgacaaatg
     3181 ccaatgctgc tgcaagggta cagccacgag acggcgtggt aaacaaggct ttaagatgag
     3241 ggaaaatgaa agccagtgca ccgaggataa agaacggcaa ataaaatagc gtttgcatga
     3301 caataaaatt gaacatgcca ttactcagaa tgggcggata cacaataaaa atcgttcttc
     3361 ttattaccgc atagccgatg ccgaggcata aaaaaatcac cgatagtttt accatcgaga
     3421 attttttatt cgttttatca gaattttcta aattatttct gatgcgttta aatatccata
     3481 cgcacagcgt cgtcatgact actaacacca gtaaaaacca caggtgtgat attaattccc
     3541 aggccaacgt attatatttg tcatacaatg acagcccagg ccaactttct gctttccctt
     3601 tgacgtattg cagcataata aattgcggca atgtcagtaa ggggatggct gttaacatcg
     3661 ggatacctac acgttcgaca cgtactttcc accatttttt caagggatag cgtaaaaaaa
     3721 gcatgtagga aaagtagccg gatataacga aaaataccag catgcggaac gagtggatga
     3781 agtcattaaa aagggtcagc cacaatgacg gttcggcgct attcacatgc catgtatggc
     3841 tcgaatagat taaagaaata tgaaaaggga tccctaacaa catcagccag gcgcggatgg
     3901 agtcgaggaa atattcacgt tgcgcgggta ctggattcat atatggttaa ctaatctcgg
     3961 atttttcgtc ttatccctgt cgggttatgc ctttaggctt gttgccatag cgacaccgac
     4021 ctgaccgcgc caggcgcagg cttcaaggtt tttatgcata gcatcatcgc taccactaac
     4081 cagaatggaa gcgtctgtaa gacggttgat aaataaaatt gctggcaaac cctacacgaa
     4141 gtcgatgctt ctgtctttag gagaagcacg gaaagtgaaa acggttgcaa tcagttgctt
     4201 aatccatgag ccagcgtgct gaacgatacc gggattctgt tgtcggaatg gctggttatc
     4261 cattaaaata gatcggatcg atataagcac acaaaggggg aagtgcttac taattatgaa
     4321 acataaacta caaatgatga aaatgcgttg gttgagtgct gcagtaatgt taaccctgta
     4381 tacatcttca agctgggctt tcagtattga tgatgtcgca aagcaagctc aatccttagc
     4441 cgggaaaggc tatgaggcgc ccaaaagcaa cttgccctcc gttttccgcg atatgaaata
     4501 cgcggactat cagcagatcc agtttaatca tgacaaagcg tactggaaca atctgaagac
     4561 cccattcaaa ctcgagttct accatcaggg tatgtacttc gataccccgg tcaaaataaa
     4621 tgaagtgact gccaccgcag tcaaacgaat caaatacagc ccggattatt tcactttcgg
     4681 cgatgttcag catgacaaag acacggtaaa agaccttggt tttgccggtt ttaaagtgct
     4741 ttacccgatc aacagcaaag ataaaaacga tgaaatcgtc agcatgctcg gggccagcta
     4801 tttccgcgtg attggtgcag gtcaggttta tggcctttct gcccgcggcc tggcaattga
     4861 taccgccttg ccatcgggtg aagaatttcc acgcttcaaa gagttctgga tcgagcgtcc
     4921 aaaaccgact gataaacgtt taaccattta tgcattgctt gactcgccgc gcgcgacagg
     4981 tgcttacaaa ttcgtggtta tgccagggcg tgacacggtt gtggatgtgc agtcgaaaat
     5041 ctatctgcgc gataaagtcg gcaaactggg ggttgcaccg ttaaccagta tgttcctgtt
     5101 tgggccgaac caaccgtcgc ctgcaaataa ctatcgtccg gagttgcacg actctaacgg
     5161 tctctctatc catgccggta atggcgaatg gatctggcgt ccgttgaata acccgaaaca
     5221 tttagcggtt agcagcttct cgatggagaa cccgcaaggc ttcggtctgt tgcagcgcgg
     5281 tcgtgatttc tcccgctttg aagatctcga tgatcgttac gatcttcgtc caagcgcatg
     5341 ggtgactccg aaaggggagt ggggtaaagg cagcgttgag ctggtggaaa ttccaaccaa
     5401 cgatgaaacc aacgataaca tcgtcgctta ctggacgccg gatcagctgc cggagccggg
     5461 taaagagatg aactttaaat acaccatcac cttcagccgt gatgaagaca aactgcatgc
     5521 gccagataac gcatgggtgc aacaaacgcg tcgttcaacg ggggatgtga agcagtcgaa
     5581 cctgattcgc cagcctgacg gtactatcgc ctttgtggtc gattttaccg gcgcagagat
     5641 gaaaaaactg ccagaggata ccccggtcac agcgcaaacc agcattggtg ataatggtga
     5701 gatagttgaa agcacggtgc gctataaccc ggttaccaaa ggctggcgtc tggtgatgcg
     5761 tgtgaaagtg aaagatgcca agaaaaccac tgaaatgcgt gctgcgctgg tgaatgccga
     5821 tcagacgttg agtgaaacct ggagctacca gttacctgcc aatgaataag acaactgagt
     5881 acattgacgc aatgcccatc gccgcaagcg agaaagcggc attgccgaag actgatatcc
     5941 gcgccgtaca tcaggcgctg gatgccgaac accgcacctg ggcgcgggag gatgattccc
     6001 cgcaaggctc ggtaaaggcg cgtctggaac aagcctggcc agattcactt gctgatggac
     6061 agttaattaa agacgacgaa gggcgcgatc agctgaaggc gatgccagaa gcaaaacgct
     6121 cctcgatgtt tcccgacccg tggcgtacca acccggtagg ccgtttctgg gatcgcctgc
     6181 gtggacgcga tgtcacgccg cgctatctgg ctcgtttgac caaagaagag caggagagcg
     6241 agcaaaagtg gcgtaccgtc ggtaccatcc gccgttacat tctgttgatc ctgacgctcg
     6301 cgcaaactgt cgtcgcgacc tggtatatga agaccattct tccttatcag ggttgggcgc
     6361 tgattaatcc tatggatatg gttggtcagg atttgtgggt ttcctttatg cagcttctgc
     6421 cttatatgct gcaaaccggt atcctgatcc tctttgcggt actgttctgt tgggtgtccg
     6481 ccggattctg gacggcgtta atgggcttcc tgcaactgct tattggtcgc gataaataca
     6541 gtatatctgc gtcaacagtt ggcgatgaac cattaaaccc ggagcatcgc acggcgttga
     6601 tcatgcctat ctgtaacgaa gacgtgaacc gtgtttttgc tggcctgcgt gcaacgtggg
     6661 aatcagtaaa agccaccggg aatgccaaac actttgatgt ctacattctt agtgacagtt
     6721 ataacccgga tatctgcgtc gcagagcaaa aagcctggat ggagcttatc gctgaagtcg
     6781 gtggcgaagg tcagattttc tatcgccgcc gccgtcgccg cgtgaagcgt aaaagcggta
     6841 atatcgatga cttctgccgt cgctggggca gccagtacag ctacatggtg gtgctggatg
     6901 ctgactcggt aatgaccggt gattgtttgt gcgggctggt gcgcctgatg gaagccaacc
     6961 cgaacgccgg gatcattcag tcgtcgccga aagcgtccgg tatggatacg ctgtatgcgc
     7021 gctgtcagca gttcgcgacc cgcgtgtatg ggccactgtt tacagccggt ttgcacttct
     7081 ggcaacttgg cgagtcgcac tactggggac ataacgcgat tatccgcgtg aaaccgttta
     7141 tcgagcactg cgcactggct ccgctgccgg gcgaaggttc ctttgccggt tcaatcctgt
     7201 cacatgactt tgtagaagcg gcgttgatgc gccgtgcagg ttggggggtc tggattgctt
     7261 acgatctccc gggttcttat gaagaattac cgcctaactt gcttgatgag ctaaaacgtg
     7321 accgccgctg gtgccacggt aacctgatga acttccgtct gttcctggtg aagggtatgc
     7381 acccggttca ccgtgcggtg ttcctgacgg gcgtgatgtc ttatctctcc gctccgctgt
     7441 ggtttatgtt cctcgcgctc tctactgcat tgcaggtagt acatgcgttg accgaaccgc
     7501 aatacttcct gcaaccacgg cagttgttcc cggtgtggcc gcagtggcgt cctgagctgg
     7561 cgattgcact ttttgcttcg actatggtgc tgttgttcct gccgaagtta ttgagcattt
     7621 tgcttatctg gtgcaaagga acgaaagaat acggcggctt ctggcgcgtt acattatcgt
     7681 tgctactgga agtgcttttt tccgtgctgc tggctccggt acgcatgctg ttccatacgg
     7741 tcttcgttgt cagcgcgttc cttggctggg aagtggtgtg gaattcaccg cagcgtgatg
     7801 atgactccac ttcctggggt gaagcgttca aacgccacgg ctcacagctg ctgttagggt
     7861 tagtgtgggc tgttgggatg gcgtggctgg atctgcgttt cctgttctgg ctggcaccga
     7921 ttgtcttctc gttgatcctg tcaccgtttg tttcggtgat ttccagccgt gccaccgttg
     7981 gtctgcgaac caaacgctgg aaactgttcc tgatcccgga agagtattca ccgccgcagg
     8041 tgctggttga taccgatcgc ttccttgaga tgaatcgtca acgctccctt gatgatggtt
     8101 ttatgcacgc ggtgtttaac ccgtcattta acgctctggc aaccgcaatg gcgaccgcgc
     8161 gtcaccgcgc cagtaaggtg ctggaaatcg cccgtgaccg ccacgttgaa caggcgctta
     8221 acgagacgcc agagaagctg aatcgcgatc gtcgcctggt gctgctaagc gatccggtga
     8281 cgatggcccg tctgcatttc cgtgtctgga attccccgga gagatattct tcatgggtga
     8341 gttattacga agggataaag ctcaatccac tggcattgcg taaaccggat gcggcttcgc
     8401 aataaaaacg tagttgcctg atgcgctacg cttatcaggc ctacatcgtt cctgcaattt
     8461 attgattttg caagattttg taggttgggt aaggcgttca cgccgcatcc ggcaaaaaca
     8521 acgagccaat aaaaataccg gcgttatgc
//
LOCUS       DAJTNA010000158         8536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTNA010000158 DAJTNA010000000
VERSION     DAJTNA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.160"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..288)
                     /locus_tag="OO161_003207"
     CDS             complement(<1..288)
                     /locus_tag="OO161_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="with NrfF and NrfG catalyzes the insertion of heme
                     into cytochrome c552; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HCR5464178.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHE"
     gene            complement(308..1264)
                     /gene="nrfD"
                     /locus_tag="OO161_003208"
     CDS             complement(308..1264)
                     /gene="nrfD"
                     /locus_tag="OO161_003208"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5464179.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMLVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPAHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(1261..1932)
                     /gene="nrfC"
                     /locus_tag="OO161_003209"
     CDS             complement(1261..1932)
                     /gene="nrfC"
                     /locus_tag="OO161_003209"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5464180.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(1929..2495)
                     /gene="nrfB"
                     /locus_tag="OO161_003210"
     CDS             complement(1929..2495)
                     /gene="nrfB"
                     /locus_tag="OO161_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5464181.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(2540..3976)
                     /gene="nrfA"
                     /locus_tag="OO161_003211"
     CDS             complement(2540..3976)
                     /gene="nrfA"
                     /locus_tag="OO161_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5464182.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWVGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            4369..6327
                     /gene="acs"
                     /locus_tag="OO161_003212"
                     /pseudo
     CDS             4369..6327
                     /gene="acs"
                     /locus_tag="OO161_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            6527..6841
                     /locus_tag="OO161_003213"
     CDS             6527..6841
                     /locus_tag="OO161_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5464183.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            6838..8487
                     /gene="actP"
                     /locus_tag="OO161_003214"
     CDS             6838..8487
                     /gene="actP"
                     /locus_tag="OO161_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5464184.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVITIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGTMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         1908 a         2358 c         2302 g         1968 t
ORIGIN      
        1 ttcatgaccg ccccacaccg ccgccagttt gagttcccac gacagcaggc tgtagctatg
       61 ttgggcgaca taaatgaccg agaaatcgct gatgagaaaa caatacgtca gtacgccaaa
      121 ggcgagcagc aggagcgcga actgcgccag aatgccgatg caagtgagtc gcatcatggc
      181 aggccagcgc aaccgcactc ccgcaaaggc cgtcaacggg gtcaacacgt tgaccccgag
      241 acttaacaac aacgccagaa aaccgacttc aggaaggaat acgtccagaa accacctctt
      301 ttctggctta cgccaccgtc aactgtccgg cgtagagaat gaaaaagcgc agcatcaaca
      361 cgccgaccag actggcccca cacgccgcca gcacggcagg aatgccggaa ctgcgattga
      421 cccacgattt gagcaacatt ggcacaatca gccccagccc ggcgacacca agccagaacc
      481 accacgtcca gaaaccgcca cctaatgccg ccaccagcgc acgaacttta ccgtcatcgc
      541 ccagcgccag accgacaaaa aacgccacca gcaggaagat ttcaccccat accacgggga
      601 tttccatccg gtgaacaaag tgcgcttccg tggaatgcgg gttactgcgt tggcgtatcg
      661 ccatggcgat cagcgccacc gccgcaccgg acgagatgcc ggagaagagg aacagcaccg
      721 gcaggatcgg gttattgagg aacggatacg atttcagcgc cgacagcaga aagccggtat
      781 aagcccccag cagcaccgcc aacaccagca tcagcgtttc cagtccgcga tgagcgggcg
      841 tcagcaggct cagaaccttt tgcacgatcc ccagcttcgg caaccagcgt tgttgcaggg
      901 caagcaaatc atgttcaaag attttcgcca gccacagcac cagcaccagc atgtaaagct
      961 gaaacagcat cacccccatc gacatcaccg aggtaaaact gtagtggaac atcagcttcc
     1021 agaaggtcca cggtcttgtc aggtggaaga cgaggatcaa cagaccgagg atcaccgcgc
     1081 ccggcccgac aatcagcgtg gtgcgcagca acgtactgtc tgcaccgccc gcctgcgggt
     1141 agaagcgacg taacagcacg gccagcgtca ccagaccggc agaaatacca atcaaaaaca
     1201 ggtagatggc aatcggccag tcccacacca gcgattcaaa atgaaatgcg gaagtctgcg
     1261 tcattggctc acctccccgt atttaaacgg tacgcggtac agcttcggtt tggttcccag
     1321 cgccagcttg tagcggtaag taggcttctg gcgcagcagt tgcgaaatct cactgttagg
     1381 atcgtccaga ttgccaaacg tcagcgcctt cgtcgggcaa gcctcaacgc acgcgggcag
     1441 cttaccggcc tgcaaattgg ttttacggca gaaatcgcat ttgtccgccg tcttcgtgac
     1501 cggatggata aagcgcacgc ggtacggaca ggcggcgatg cagtactgac aaccgacgca
     1561 gagatcagga ttaacatcga caatcccgct agcggcatca cgaaaagacg caccggtcgg
     1621 gcagacgtca acgcacggcg catgatcgca gtgctggcaa gacttacgga agaagcgata
     1681 tttcacatca ggaaattcgc cctgcggctc gctacgaata atcgtcaggc gcgagacgcc
     1741 ttccggcacc ttgttcactt ctcgacaagc atccatacag gcggtacagc cgatgcataa
     1801 cgactcgtca tgcaccatgc cgtaacgcac cccattgata ttcaacgttt tcgccaccac
     1861 gcgccctgcg gtgccgctga cggctgccag cacgccgacg ccggtgagaa actggcgacg
     1921 agaccaggtc atggctgctc cttaagcaac ggaacagacg ccgggttaaa gttcggattg
     1981 gtgcgctgat cgctgtggca atcgacgcaa atcttaatcc gtcctttgtc acttaacgtc
     2041 tgcatcgtat cttgctgtgg atgcagggaa tggcagctgg cgcacgccac tttggttacg
     2101 tggacatcgt gcggccagaa cgctttttgc aactgttccg gcagatgaca ggacatacag
     2161 acgctgttct gctctccgac cttgtacatc ggctcgttaa agcgcatcac atctttcacc
     2221 ccttcgcggt gttgcggtga tggctggccg tgacagttgg tgcaggtgac cggcaatttg
     2281 ttattcgggt tgataacgga agcatgtttg ccatgcatac cttcggtatc tggtttatga
     2341 cagtccagac aggcggcatc cgggttacgc tgctgggtaa cttcgtagcg atcaccagat
     2401 gcctgcgccg caggggtagc ggtaattccg ttcaggctcc acaacaggcc cgacgccagc
     2461 accccagcag ttaataacga acgtaatacg ctcatattca ctccattgtg ttttccgccc
     2521 cttgcgaggc ggaacggggt tattggctta acagaccgtt tttacgtgcc tgttcttccc
     2581 actgcgggat caccgttttg atgaagtcct gcttctcggc cttgatttgt tccatgttca
     2641 ggccaatggc ctgctgggct ttctctttgg ttgagatatc cgggatctgg atttcatggg
     2701 tgatgccttt ggtcgccagc aggcgcgcca gtttggtgcg tgcatccgcc gctttatcca
     2761 tcgccgtacc gagcatccgt aaaccttctt ccggtgcgtg catatgaatg ccgtgggaag
     2821 cgatcgccag atcccagcgc cactgggcat gacggatatc gtcctgaatt ggcttcattt
     2881 cagcttccgt cgcgcctgca tccagcgctg ctttcgcttc gaagtgagcg tgaaccagtt
     2941 gatcttcaac cttgattttc aggtcgttaa tcgactgctt acgttccgcg accacttttt
     3001 gcaaggcagc tttgtcctgg gtatggcagt tcgcacaagt ctgggcgaag ttatcaaacg
     3061 gattaccaat tttatggtcg gtgtagagtt tgccttcggc gttctgcact tttggcatat
     3121 ggcagtcgat acaggtcacg ttgtttttac cgtgaatgcc cgctgtccag gtttcatatt
     3181 ccgggtgctg cgctttcagc attggcgttt tcgacaggga gttagtccag tcagagaagg
     3241 caattttgtc gtaatactgc tccatatttt cgactttcat gccgtcatcc cacgggaatt
     3301 taaccgcttt gtttttgccg tcgaagtaat actccacatg gcactgaccg caaaccatcg
     3361 attgctggtc gaaacgtccg gctttctcaa aaggtttacc aatggcttcc atcgcgcgag
     3421 ccgcatacgg gcgggaaagg gttaactccg gtttgccttt ggcgaactct ggtgaggcgg
     3481 tgttatggca gtcggcacaa cctaagttgt tgacgatttc cgggccgccg cgcgcccact
     3541 taccgtggaa gtagccatct tcgccgtctt tctggatcag acgcgccaca tccgggcttt
     3601 tacaactcca gcacgccatc ggtagcggac catcttcagc gtttttcggc gcaccggtac
     3661 gcagggtttc acgcacatcg gtcacagcaa aggcatgtcc acgcggcttg ttgtaatcgc
     3721 gcgagaaggg ataccccacc cacaggatca ccagccgtgg atcttccgcc agggcgtcaa
     3781 cacgctctga ctgttccgag gtggctttcc aggagagata ttgatcggga tgctgcgggg
     3841 caaaggtttc attcttcgct tccacagtta caggttttgc gggagctgcc gtttgttcag
     3901 cgtgaacaga agtgaaaaag aaaaaaggaa tcaataagct gaagatacgg cgtgcgttta
     3961 tttttatcct tgtcataggg gcttcatccg aattgcgcca ttgttgcaat ggcggttttt
     4021 attgtttttc acgacagtaa ccgcacctac actgtcatga cattgctcgc ccctatgtgt
     4081 aacaaataac cacactgtga atgttgtctt taatcaattg taagtgcatg taaaatacca
     4141 ctttagagtt agtcagtatc ttcctctttt tcaacagcat gcataactgc atgttcctca
     4201 aagaattaat caacttttgt tgctgacctt caaaaattac cctgccgttt atttgcacaa
     4261 ttctactttt gcgtgatctg tcgcccaaat actaaacaaa actgccaata cccctacatt
     4321 taacgcttat gccacatatt attaacattc tacaaggaga acaaaagcat gagccaaatt
     4381 cacaaacaca ccattcctgc caacatcgca gaccgttgcc tgataaaccc tcagcagtac
     4441 gaggcaatgt atcaacaatc tattaacgta cctgatacct tctggggcga acagggaaaa
     4501 attcttgact ggatcaaacc ttaccagaag gtgaaaaaca cctcctttgc ccccggtaat
     4561 gtgtccatta aatggtacga ggacggcacg ctgaatctgg cggcaaactg ccttgaccgc
     4621 catctgcaag aaaacggcga tcgtaccgcc atcatctggg aaggcgacga cgccagccag
     4681 agcaaacata tcagctataa atagctgcac cgcgacgtct gccgcttcgc caataccctg
     4741 ctcgagctgg gcattaaaaa aggtgatgtg gtggcgattt atatgccgat ggtgccggaa
     4801 gccgcggttg cgatgctggc ctgcgcccgc attggcgcgg tgcattcggt gattttcggc
     4861 ggcttctcgc cggaagccgt tgccgggcgc attattgatt ccaactcacg actggtgatc
     4921 acttccgacg aaggtgtgcg tgccgggcgc agtattccgc tgaagaaaaa cgttgatgac
     4981 gcgctgaaaa acccgaacgt caccagcgta gagcatgtgg tggtactgaa gcgtactggc
     5041 gggaaaattg actggcagga agggcgcgac ctgtggtggc acgacctggt tgagcaagcg
     5101 agcgatcagc accaggcgga agagatgaac gccgaagatc cgctgtttat tctctacacc
     5161 tccggttcta ccggtaagcc aaaaggtgtg ctgcatacta ccggcggtta tctggtgtac
     5221 gcggcgctga cctttaaata tgtctttgat tatcatccgg gtgatatcta ctggtgcacc
     5281 gccgatgtgg gctgggtgac cggacacagt tacttgctgt acggcccgct ggcctgcggt
     5341 gcgaccacgc tgatgtttga aggcgtaccc aactggccga cgcctgcccg tatggcgcag
     5401 gtggtggaca agcatcaggt caatattctc tatactgcac ccacggcgat ccgcgcgctg
     5461 atggcggaag gcgataaagc gatcgagggt accgaccgtt cgtcgctgcg cattctcggt
     5521 tccgtgggcg agccaattaa cccggaagcg tgggagtggt actggaaaaa aatcggcaac
     5581 gagaaatgtc cggtggtcga tacctggtgg cagaccgaaa ctggcggttt catgatcacg
     5641 ccactgcctg gcgctaccga gctgaaagcc ggttcggcaa cacgtccgtt cttcggcgtg
     5701 caaccggcgc tggtcgataa cgaaggtaac ccgctggaag gcgctaccga aggcagcctg
     5761 gtgatcaccg actcctggcc gggtcaggcg cgtacgctgt ttggcgatct cgaacgtttt
     5821 gaacagacct acttctccac cttcaaaaat atgtatttca gcggcgacgg cgcgcgtcgc
     5881 gatgaagatg gctattactg gatcaccggg cgtgtggacg acgtgctgaa cgtctccggt
     5941 caccgtctgg ggacggcaga gattgagtcg gcgctggtgg cgcatccgaa gattgccgaa
     6001 gccgccgtag taggtattcc gcacaatatt aaaggtcagg cgatctacgc ctacgtcacg
     6061 cttaatcacg gggaggaacc gtcaccagaa ctgtacgcag aagtccgcaa ctaggtgcgt
     6121 aaagagattg gcccgctggc gacgccagac gtgctgcact ggaccgactc cctgcctaaa
     6181 acccgctccg gcaaaattat gcgccgtatt ctgcgcaaaa ttgcggcggg cgataccagc
     6241 aacctgggcg atacctcgac gcttgccgat cctggcgtag tcgagaagct gcttgaagtg
     6301 aagcaggcta tcgcgatgcc atcgtaaccc acaattgccg gatgcgagtc ggtaacggtt
     6361 tgtaggcctg ataagacgcg acagcgtcgc atcaggcatt gattgccgga tgcggcgtaa
     6421 acgccttatc cggcctacat tcggcaaggg ttacccgagc gttaaccttc tcccataagg
     6481 gagcgggaat taaaacaatc cctacattac ctctggagaa tctgtgatga atggcactat
     6541 ttatcagcgg atagaagaca atgcgcattt cagggagtta gtcgaaaaac ggcaacggtt
     6601 tgccaccatc ctgtcgatta ttatgctggc agtttatatc ggctttattt tactgatcgc
     6661 cttcgcgccc ggctggctgg gcacgccgct gaatccgaac accagcgtca cacgcggtat
     6721 tccaattggt gttggagtga ttgtgatctc ctttgttctc accggtatct acatctggcg
     6781 ggcgaacggc gaattcgacc gtcttaataa tgaagtcctg catgaggtac aagcatcatg
     6841 aaaagagttc tgacggcgct tgccgccaca ctccctttcg cagctaacgc cgcggatgct
     6901 attagcgggg ccgtagagcg ccagccaacg aactggcagg cgattattat gttcctgatt
     6961 ttcgtcgtgt ttacgctcgg cattacctac tgggcatcaa aacgcgtacg ttctcgtagc
     7021 gactactaca cggcaggcgg caatatcaca ggcttccaga acgggctggc gattgccggt
     7081 gactatatgt ccgccgcctc atttttgggg atctccgcgc tggtgtttac ctccggctat
     7141 gacggcttaa tttactcgct gggcttcctg gtgggctggc cgatcatttt gttcctgatt
     7201 gccgaacgtc tgcgtaacct gggacgctac acctttgccg atgtagcctc ttaccgcctg
     7261 aaacaagggc cgattcgtat tctttcggcc tgtggttctc tggtggtggt ggcgctttac
     7321 cttatcgccc agatggtagg cgcaggtaaa ctgatcgagc tgctgtttgg ccttaactat
     7381 cacattgcgg tggtgctggt cggcgtgctg atgatgatgt acgtcctgtt cggcggcatg
     7441 ctggcgacca cctgggtgca aattattaaa gccgtgctgt tgctgttcgg tgccagcttt
     7501 atggccttta tggtgatgaa acacgtcggc tttagcttca ataatctgtt cagcgaagcg
     7561 atggcggtac acccgaaagg tgtcgacatc atgaagccgg gcgggctggt gaaagatccg
     7621 atctccgcgc tctctctggg tctgggactg atgtttggta cggcgggctt gccgcacatt
     7681 ctgatgcgct tctttacagt cagcgatgcc cgcgaagcac gtaagagcgt gttctacgcc
     7741 accgggttta tgggctactt ctatattctg acctttatta tcggcttcgg cgcgatcatg
     7801 ctggttggtg cgaatccgga atataaagac gcggcgggcc atctgattgg tggtaacaac
     7861 atggcggccg ttcacctggc gaatgcagtg ggcggcaacc tgttcctcgg ttttatttca
     7921 gcggttgctt tcgccactat cctcgcggtg gttgcgggtc tgacgctggc gggcgcatcc
     7981 gcggtttcgc atgacttgta cgctaacgtc ttcaaaaaag gtgcgaccga acgtgaagaa
     8041 ctgcgggtat cgaaaatcac cgtcctgatt ttgggtgtga ttacgattat cctcggcgtg
     8101 ctgtttgaga atcagaacat cgcctttatg gtaggccttg cgtttgccat cgcggcgagc
     8161 tgtaacttcc cgatcattct gctttccatg tactggtcga aactgactac ccgtggcacg
     8221 atgctgggcg gctggctggg gctgattacc gcagtggtgc tgatgatcct cggcccgact
     8281 atttgggtac agatcctcgg tcacgaaaaa gccatcttcc cgtatgaata cccggcgctg
     8341 ttctctatca gcgtggcatt cctcggcatc tggttcttct cggcaaccga taactcagcg
     8401 gaaggtgcgc gcgagcgtga actgttccgt gcgcagttta tccgctccca gaccggcttt
     8461 ggcgttgagc aaggccgcgc acattaatct ctccccttcc ccggtcgcct gaccggggaa
     8521 tactcttcct ctccag
//
LOCUS       DAJTNA010000159         8520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTNA010000159 DAJTNA010000000
VERSION     DAJTNA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.161"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..207
                     /locus_tag="OO161_003215"
     CDS             <1..207
                     /locus_tag="OO161_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5464185.1"
                     /translation="QTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRD
                     GESSPAEYSVSLTRADMIEFTMEH"
     gene            208..660
                     /gene="phnG"
                     /locus_tag="OO161_003216"
     CDS             208..660
                     /gene="phnG"
                     /locus_tag="OO161_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR5464186.1"
                     /translation="MHADIATRQHWMSVLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            657..1241
                     /gene="phnH"
                     /locus_tag="OO161_003217"
     CDS             657..1241
                     /gene="phnH"
                     /locus_tag="OO161_003217"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR5464187.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            1241..2305
                     /gene="phnI"
                     /locus_tag="OO161_003218"
     CDS             1241..2305
                     /gene="phnI"
                     /locus_tag="OO161_003218"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR5464188.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            2298..3143
                     /gene="phnJ"
                     /locus_tag="OO161_003219"
     CDS             2298..3143
                     /gene="phnJ"
                     /locus_tag="OO161_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR5464189.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            3140..3898
                     /gene="phnK"
                     /locus_tag="OO161_003220"
     CDS             3140..3898
                     /gene="phnK"
                     /locus_tag="OO161_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR5464190.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVKIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSFLQN"
     gene            4009..4689
                     /gene="phnL"
                     /locus_tag="OO161_003221"
     CDS             4009..4689
                     /gene="phnL"
                     /locus_tag="OO161_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR5464191.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            4686..5822
                     /gene="phnM"
                     /locus_tag="OO161_003222"
     CDS             4686..5822
                     /gene="phnM"
                     /locus_tag="OO161_003222"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR5464192.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQVALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            5822..6379
                     /gene="phnN"
                     /locus_tag="OO161_003223"
     CDS             5822..6379
                     /gene="phnN"
                     /locus_tag="OO161_003223"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR5464193.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            6366..6800
                     /gene="phnO"
                     /locus_tag="OO161_003224"
     CDS             6366..6800
                     /gene="phnO"
                     /locus_tag="OO161_003224"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR5464194.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            6802..7560
                     /gene="phnP"
                     /locus_tag="OO161_003225"
     CDS             6802..7560
                     /gene="phnP"
                     /locus_tag="OO161_003225"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR5464195.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(7707..8036)
                     /gene="yjdP"
                     /locus_tag="OO161_003226"
     CDS             complement(7707..8036)
                     /gene="yjdP"
                     /locus_tag="OO161_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR5464196.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(8105..8422)
                     /locus_tag="OO161_003227"
     CDS             complement(8105..8422)
                     /locus_tag="OO161_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR5464197.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         1808 a         2593 c         2473 g         1646 t
ORIGIN      
        1 caaaccggga ttgcgctgcg ccgcagccag acgcggatca gcgcccgccg cgcccaggcc
       61 aaagagtgcc agcgtcttga aatcccgaat atgtcgccgc tgctgtgcgt gcgcaccctt
      121 aaccaccgtg acggtgaaag cagcccggcg gagtactccg tcagcctgac gcgcgccgac
      181 atgattgaat tcactatgga gcactgaatg cacgcagata tcgcgacccg ccagcactgg
      241 atgtccgtgc tggcgcacag ccaaccggct gaactggcag cccgcctgaa cgcgctgaac
      301 atcaccgcca actatgaagt gatccgcacc gccgaaaccg gcctggtaca gattcaggcg
      361 cgaatgggcg gtaccggcga acgctttttt gccggcgacg ccacgctgac ccgcgccgcc
      421 gtacgcctga ctgacggcac cctcggctac agctgggtgc tggggcgcga caaacagcac
      481 gccgaacgct gcgcgctgat tgacgcgttg atgcagcaat ctcgccattt tcaaaactta
      541 tcagaaaccc ttattgcccc actggacgct gaccgtatgg cacgcattgc cgcacgccag
      601 gccgaagtga acgccagccg ggtcgacttc tttacgatgg ttcgcggaga caacgcatga
      661 ccctggaaac cgcttttatg cttcctgtgc aggatgccca gcacagtttt cgtcgcctgt
      721 taaaggccat gagcgagccg ggcgtgattg tcgccctgca tcagctcaaa cgcggctggc
      781 agccgctgaa tatcgccacc accagcgtgc tgctgacgct ggctgataac gacacgccgg
      841 tgtggctttc cgcgccatta agtaacgata tcgtcagcca gagcctgcgt tttcatacca
      901 acgcgccgct ggtcaatcag ccggaactgg cgaccttcgc ggtaacggat gaggcgattt
      961 ccagcgaaca gctcaacgct ctttccagcg gcaccgctgt tgcgccggaa gcgggcgcga
     1021 cgctgatttt acaggtcgtc agcctgagcg gcgggcgcat gctgcgcctt actggcgcgg
     1081 gtattgccga agaacgaatg atcgctccgc agctgccgga gtgcattctg cacgaactca
     1141 ccgagcgccc gcacccgttc ccgctcggca tcgacctgat cctgacctgc ggcgagcggc
     1201 tgctggctat cccgcgaacc actcatgtgg aggtgtgctg atgtacgttg ccgtgaaagg
     1261 gggcgagaag gagatcgacg ccgctcacgc cctgcaagag agccgacgcc ggggcaatac
     1321 cgatttgcct gaactgagcg tcgcccagat tgaacagcag cttaatcttg cagtagatcg
     1381 cgtgatgacc gaaggcggca ttgccgaccg cgaactggcg gcgctggcgc tgaaacaggc
     1441 cagcggcgat aacgtcgaag cgattttcct gctacgcgcc taccgcacca cgctggcgaa
     1501 gctggcggta agcgagccgc tcgacaccac ggagatgcgt ctcgaacggc gtatttcagc
     1561 ggtttataaa gatattcccg gcggtcagtt gcttggccca acctacgact acacccatcg
     1621 cctgctcgat ttcaccctgc tggcaaacgg cgaagcgccg acgctgacca ccgcagacag
     1681 cgaacaatcc ccgtcgccgc acgttttcag cctgctggcg cgtcaggggc tggcgaagtt
     1741 tgaagaggat agcggcgcac agccggatga catcacccgc acgccgccag tttacccctg
     1801 ttcacgttct tcccgtttgc agcagttgat gcgcggcgac gaaggctatt tgctggcgct
     1861 ggcctactct acccagcgcg gttacggacg caatcacccg ttcgcgggcg aaatccgcag
     1921 tggttacatc gacgtgtcga ttatgccgga agagctggga tttgcggtga acgtcggcga
     1981 actgctgatg accgagtgtg aaatggtcaa cggttttatt gacccgccgg atgagccgcc
     2041 gcacttcacg cgcggctacg ggctggtgtt cggcatgagc gagcgcaaag cgatggcgat
     2101 ggcgctggtt gaccgtgcgt tgcaggctcc ggagtacggc gagcacgcga caggcccggc
     2161 gcaggatgaa gagttcgtgc tggcacatgc cgacaacgtc gaagccgcag gctttgtctc
     2221 gcacctcaaa ctcccccact acgtcgattt ccaggccgaa ctggagctac tcaaacgtct
     2281 gcaacaggag cagaaccatg gctaatctga gcggctacaa ctttgcctac ctcgacgagc
     2341 agaccaaacg catgatccgc cgcgccatct taaaagcggt ggcgatcccc ggttatcagg
     2401 tgccgtttgg cgggcgcgag atgccgatgc cctacggctg gggaaccggc ggcattcagc
     2461 tcaccgccag cgtgattggc gaaagcgacg tgctgaaggt gattgaccag ggcgcagacg
     2521 acaccaccaa cgccgtgtcg attcgcaact tctttaagcg cgtgaccggg gtaaacacca
     2581 ctgaacgtac ggacgatgcg acgcttatcc agacgcgcca tcgcatcccc gaaacgccgc
     2641 tgaccgaaga tcagatcatt atcttccaag tgccgatccc cgaaccgctg cgctttatcg
     2701 agccgcgcga aacggaaacc cgcaccatgc acgcgctgga agagtacggc gtgatgcagg
     2761 tgaaactgta tgaagatatc gcccgcttcg gtcatatcgc cactacctac gcctatccgg
     2821 tgaaggtgaa cgggcgctac gtgatggacc cgtcgccgat cccgaaattc gataacccaa
     2881 aaatggacat gatgcccgcc ctgcaactgt tcggcgcggg gcgcgagaag cgcatctatg
     2941 cggtgccgcc gtttacccgc gtggaaagtc tcgatttcga cgatcacccg ttcaccgttc
     3001 agcagtggga tgagccatgc gccatctgcg gatcgaccca cagctatctt gatgaagtgg
     3061 tgctggatga cgccggaaac cgcatgtttg tctgctccga taccgattat tgccgccaac
     3121 agagcgaggc aaaaaaccaa tgaatcaacc gttactttcg gtcaataacc tgacccacct
     3181 ttacgcgccg ggcaaaggct ttagcgatgt ctcttttgat ttatggccgg gggaagtgct
     3241 gggcattgtc ggggagtccg gctccggaaa gaccacgctg ctgaaatcaa tctccgcgcg
     3301 cctgacgccg cagcaggggg agattcacta tgagagccgt tcgctgtacg caatgagcga
     3361 ggccgaccgc cgtcgcctgc tgcgcaccga atggggcgta gtgcatcagc atccgctcga
     3421 cggcctgcgc cgccaggtgt cggcaggcgg caatatcggc gagcggctga tggcgaccgg
     3481 ggcacgtcat tacggcgata ttcgtgccac cgcgcagaag tggctggaag aggtgaaaat
     3541 tcccgccaac cggatcgacg acctgccgac caccttttcc ggcggcatgc agcaacgttt
     3601 gcagatcgcc cgcaacctgg tgacgcatcc gaagctggtg tttatggacg agccgaccgg
     3661 cggactggat gtgtcggtac aggcacgcct gctcgacctg ctgcgcggcc tggtggtgga
     3721 gctgaacctc gcggtagtga ttgtcaccca cgatttaggc gtcgcccgcc tgctggcgga
     3781 ccgtttgctg gtgatgaagc aggggcaagt ggtggagagt gggttaaccg accgcgtgct
     3841 cgacgacccg caccatccgt atacacaact gctggtgtca tcgtttttgc agaattgagc
     3901 cggtgccgga tgcggcgtaa acgccttatc cggcctacaa atgcgctccc cgtaggtcgg
     3961 ataagacgcg tcagcgtcgc atccgacacc cgaaccacga ggcgaaaaat gattaacgta
     4021 caaaacgtca gtaaaacctt catcctgcat cagcaaaacg gcgtacgcct gcccgtctta
     4081 caacgtgcct cgctcaccgt caacgcaggc gaatgcgtgg tgctccacgg tcactccggc
     4141 agcggcaaat caaccctgct gcgctcgctg tacgccaact atctacccga cgaaggtcaa
     4201 atccagatca aacacggtga cgagtgggta gacctggtca ccgcgccagc gcgcaaagtg
     4261 gtggaaatcc gcaaaaccac agtcggctgg gtgagccagt ttctgcgcgt catcccgcgt
     4321 atctcagcac tggaagtggt gatgcagccg ctgctcgata ccggcgttcc gcgtgaagcc
     4381 tgcgccgcta aagccgcgcg tcttctcacc cgcctgaacg tgccggaacg cctgtggcac
     4441 ctggcaccat cgaccttctc cggcggcgaa cagcagcgcg tcaacatcgc ccgcggcttt
     4501 atcgtcgact accccattct gctgctcgac gaacctaccg cctcgctgga cgccaaaaac
     4561 agcgccgcgg tggtggaact gattcacgaa gccaaagccc gtggcgcagc catcgtcggc
     4621 atcttccatg acgaagctgt acgtaatgac gtcgccgacc gcctgcaccc aatgggagcc
     4681 tcttcatgat tatcaataac gttaagctgg tgctggaaaa cgaggtggtg cacggttcgc
     4741 tggaggtgca ggatggcgaa atccgcgcct ttgccgaaag ccagagccgc ctgccagaag
     4801 cgatggacgg cgaaggtggc tggctactac ccggcctgat tgagctgcat accgacaacc
     4861 tcgataaatt cttcaccccg cgcccgaaag tcgactggcc cgcccattcg gcgatgagca
     4921 gccacgacgc gctgatggtg gcaagcggca tcaccaccgt gctggacgcg gtggcgattg
     4981 gcgacgtgcg cgacggcggc gatcggctgg agaatctgga gaagatgatc aacaccatcg
     5041 aagagacgca gaaacgcggc gtcaaccgcg ccgagcaccg tctgcacctg cgctgcgagc
     5101 tgccgcatca caccacactg ccgctgtttg aaaaactggt tcagcgcgaa ccggtgacgc
     5161 tggtgtcgct gatggaccac tcaccgggcc agcgccagtt cgctaaccgc gagaagtatc
     5221 gcgaatatta tcagggcaaa tactccctca ccgatgcgca gatgcagcag tacgaagaag
     5281 agcaattggc gctcgccgca cgctggtcgc agccgaatcg cgaatccatc gccgccctgt
     5341 gccgcgcgcg acaagttgcg ctcgccagcc acgatgacgc cacgcacgcc cacgttgccg
     5401 aatcccacca acttggcagc gtgatcgccg aatttaccac cacgttcgaa gcggcggaag
     5461 cctcgcgtaa gcatggcatg aacgtgctga tgggtgcgcc aaatatcgtg cgtggcggct
     5521 cgcactccgg caatgtggcg gccagtgaac tggcgcagct tggtctgctg gatatcctct
     5581 cttccgacta ctaccccgcc agcctgctgg atgcggcgtt ccgcgtcgcc gatgacgaga
     5641 gcaaccgctt tacgctaccg caggcggtga ggctggtgac caaaaatccg gcgcaggcgc
     5701 tgaatcttca ggatcgcggg gtgattggcg agggtaaacg cgctgacctg gtgctggcgc
     5761 atcgccaggg caatcacatt catatcgacc acgtctggcg tcagggtaaa agggtgttct
     5821 gatgatggga aaactgattt ggttaatggg gccgtccggc tccgggaaag acagcctgct
     5881 ggcggaactc cgcctgcggg aacaaacgca gttactggtg gcgcatcgct acatcacgcg
     5941 cgacgccagc gccggaagtg aaaaccatat cgccctgagt gagcgggagt tttttacccg
     6001 cgcggggcaa aacctgctgg ccttaagctg gcacgctaac ggtctgtatt atggcgtcgg
     6061 catcgagatt gacctctggc tgcacgctgg attcgacgta gtcgtcaacg gttcacgcgc
     6121 gcatttgccg caggcacggg cgcgctatca atcggcgctg ctgcccgtct gtttacaggt
     6181 ttcgccggag atcctgcgcc agcgcctgaa aaaccgtggc cgtgaaaaca ccagtgaaat
     6241 taacgcccgg ctggcgcgcg ccgcccgcta tactccacag gattgccata cgctcaacaa
     6301 tgacggcagc ctgcgccagt cggtcgacac actgctgacg ctgattcatc agaaggagaa
     6361 acaccatgcc tgcttgtgag cttcgcccgg ccacgcagta cgataccgac gcggtttacg
     6421 cgctgatttg tgagctaaaa caggcggagt ttgaccacca cgcgtttcgc gtgggcttta
     6481 acgccaacct gcgcgacccg agcatgcgct accatctggc gctgctggat ggcgaagttg
     6541 tcggcatgat cggcctgcat ttgcagtttc atctgcatca tgtcaactgg atcggcgaaa
     6601 ttcaggaact ggtggtgatg ccgcaggcgt gcggtctgaa cgtcggcagt aagttactgg
     6661 cgtgggcaga agaagaagcc cgccaggccg gggccgaaat gaccgaactt tccaccaacg
     6721 tgaagcgcca cgacgcgcac cgtttttacc tgcgtgaagg ctacgagcag agccacttcc
     6781 gcttcaccaa ggcgctgtaa catgagcctg accctcacgc tcaccggcac cggcggcgca
     6841 cagggcgttc cggcatgggg ctgcgagtgc gtagcctgcg ccagagcgcg gcgctcaccg
     6901 cagtatcgcc gccagccatg cagcggcgta gtgaagttta acgacgcaat caccctgatc
     6961 gacgccgggc tgcacgatct cgccgatcgc tggtcgcccg gatcgttcca gcagtttttg
     7021 ctgacgcact accatatgga tcacgtgcag gggctattcc cgctgcgctg gggcgtgggc
     7081 gatccgatcc cggtttacgg cccgccggat gaacagggct gcgacgatct gtttaaacat
     7141 ccgggcctgc ttgatttcag ccacacggtg gaaccgtttg tggtgtttga tttgcagggg
     7201 ttacaggtca cgcccctgcc gctcaaccac tcaaaactga ccttcggtta tctgctggaa
     7261 acggcacaca gccgggtggc gtggctgtct gacaccgcag gcttgccgga aaaaacgctg
     7321 aaatttttac gcaataatca gccgcaggta atggtgattg attgcagcca cccgccgcgc
     7381 gaggatgcgc cacgtagtca ctgtgattta aacaccgtgc tggcgctgaa tcaggttatc
     7441 cgctcgccac gggtgattct gacccatatc agccaccagt ttgatgcgtg gctgatggaa
     7501 aacgcactgc cgtcggggtt tgaggcgggg tttgatggga tggagattgg ggtggcgtga
     7561 tgagagggaa tgtgcgcgct ggccccctca ccctaaccct ctccccagag gggcgagggg
     7621 accgattgta ctcgatattg aatattgcgc tggttttctc cctctcccca ttggggtgag
     7681 gggcgatgcc tgctccatac ccaacctcat cgcccatact catcttccat tctccgctct
     7741 tcatcttcta gctgccgacg ctcctgatca agctggcgct ggcgatcgtc cagctgcctg
     7801 cggcgatctt caaactggcg gcggcggtcg tcatattgtc tgcgccgatc gtcgctcact
     7861 tcacgctgcc agccgtcgtc gcgcgaatct tcatagtctc gcccacggtc agggttataa
     7921 gcgtcgttaa tcgcctgctg aatattgcca atggtgtcgt cgataatatc ggcctgggcc
     7981 ggaacggtgg acagcgtgag cagggtgaat aaaagaaata gcggaaagcg tttcattagc
     8041 caacctcaaa aagaaactct atccacatta atcattactc atccatgcaa gtagtggatg
     8101 aatctcaatt tctccgctgc tctattgccg taatcgcctc cacgcgttgt tgatgacgac
     8161 cgccttcgta ctgtgcgccc agccacgcat ccacaatcat ttttgccagt tcgaggccaa
     8221 ccactcgtga accaaaagcc agtgcaactt cactggcata atgcggataa tcagtgcgct
     8281 ctgacgacct ggtgtcttta tcaatcacct cactcattga ccatgaataa aaggctggca
     8341 cagcaacgca gaatcctgcg gtgccagcga catgaccgcc cattgctccg gtacgttttt
     8401 taaaaagaaa caccggttgc attgataaat tgtgctcagt atttgccgaa gtacaatata
     8461 tataataaat atataaaaca cctcatatta tttacagaga taataaattc gcgtaatttt
//
LOCUS       DAJTNA010000160         8483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTNA010000160 DAJTNA010000000
VERSION     DAJTNA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.162"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..104)
                     /locus_tag="OO161_003228"
     CDS             complement(<1..104)
                     /locus_tag="OO161_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR5464198.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRD"
     gene            complement(124..4994)
                     /gene="traI"
                     /locus_tag="OO161_003229"
                     /pseudo
     CDS             complement(124..4994)
                     /gene="traI"
                     /locus_tag="OO161_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            complement(4981..5400)
                     /locus_tag="OO161_003230"
     CDS             complement(4981..5400)
                     /locus_tag="OO161_003230"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5464199.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            5482..5709
                     /gene="vapB"
                     /locus_tag="OO161_003231"
     CDS             5482..5709
                     /gene="vapB"
                     /locus_tag="OO161_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5464200.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            5709..6107
                     /gene="vapC"
                     /locus_tag="OO161_003232"
     CDS             5709..6107
                     /gene="vapC"
                     /locus_tag="OO161_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5464201.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YDAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(6116..8296)
                     /gene="traD"
                     /locus_tag="OO161_003233"
     CDS             complement(6116..8296)
                     /gene="traD"
                     /locus_tag="OO161_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HCR5464202.1"
                     /translation="MSFNAKDITQGGQITSMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGRDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLYKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQERP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVV
                     DMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         1739 a         2632 c         2160 g         1952 t
ORIGIN      
        1 atatcccgct gtccgggtga ccagaccaga aatgactgca accaggtatc ggtccgtgca
       61 gccagtgaat cgttacgtgt cgtgttcgtg ttatccgttg tcatcagtgt ttgtcttcct
      121 gtatcagtct ccgcccgggg ttttctcttt ctgcaggtca cgaaccatat cccgctccat
      181 ctgctgcaga cgctcctgca gcaaatggag cgggcaactt cccggaccgc ttcccgcacc
      241 cgttgtggtt cacgcagtac actctccggc agcgcggctt cccgttcagt tatggcagcc
      301 cggtcacgct cctgcccggc aatttccctg acagcctgct ctgttttgcc gtccggcagg
      361 tcaggtttgt tctctgccat tttacggaca atttcatctg cgcgacgttc cgtttcacgg
      421 cggatggcct cttcagcctg ccgctgtgcc agtacctctg ccgtgaccgg ttcgccattt
      481 ccggctcccg gctggacgct gttgtccggg atatcccccc acacgcggcc accggtgatg
      541 gcaccgggat tccacggacg gccttcaccg gcgattctta ccaccacacc actgtcagga
      601 ttatcacggg caatccggac gccatcctgc atattatcag ccagcaggct ttctccgtta
      661 cggctgccct gcagggccac gaactgcgca tccccgctgc cttttacccg accttcaccg
      721 ctgaatcccc gcagcccgtt tccgtcatcc gtggtcagcg ggttcagcca gataccggcg
      781 gacttgccgt tacggtcaaa cgccggcagt gccacatacg gttgcggata tttacgtccc
      841 ggagcaataa accgtgcagg actgtctccc ccggccagcc ccgcctgacg gagaacggca
      901 cggcctgccg ccacgtcccg cagctcccgc gccgtactga acagccgctc tgcattcatg
      961 acctcccggt ccggtttcgg ctcaaacaca tcgtgggcag ttcctttctg tacggcattg
     1021 ttgatggcat ccgtccagcc ctgacggtta tcggtgtaca cctgcacatg ctgcttcata
     1081 cgcgacaggg ccacgtaggc tgactcaaag ccggccatca gtttccggtt accttccgtg
     1141 ccttcaagcg cgatggcaaa ggtttcactc gccccctgtg caccgtgggc ggtgatggca
     1201 taggccaggt caatatgttg ctctgcccgc tcctgaccgg ggcgaatcac acgggtctgc
     1261 tgaccgtcag aaagtgtgac actgtcaccg gaaaccgctg tcaccgtcca gacgctgttg
     1321 gccacataac cgcgctcccg gtcactcttc gtgaagcgta tcctgtcacc ggtccccacc
     1381 cggatggtgt ccggggtgta cagcgtgacg ccttcagcca ccgcctcccg gggcgaaatc
     1441 agccgcgtgt taccttccgc atcctgcagg gttatcagcc cgtcatcctt gctgataccg
     1501 gcaatccggt gatacacatt atccaccagg gcaagggcat ccgggttatt ctcccaggtg
     1561 gagagacgac gcagctcccc gtcgcgtata ttcgctgtgt tcaggacagg caccatgacc
     1621 tgcacctgcc ccagttctcc ggccttttct cgtgcatcat gaatcatgct gttcagtacg
     1681 cgccggtcct cattcaggtg cgtgacaatc agcgtctgct cccgtgcttc cggtgtcctg
     1741 ccggtatagt cgcggacaat ggcttcatac agtgtcatgg ggacatccgg aaaagcctcg
     1801 cctttcagca tcgccttctg ctgcgcttct gccagtttcg cttcctggct gtgactgaac
     1861 tccgtcacgg agtgctccgg tgcccatgcg ccctccagac gtggcacctg agacggtttc
     1921 acacgctcaa gcccggacag cgccctttcc acatcccggt taatcaggct gtataccgcc
     1981 tcccgcagtt ccggcgtctg acgcacaatc tccttcatga tgaccacatc ggcagcactg
     2041 cgcgtctgct ggagacggaa aggctgaccg ggcgcgatgg cctgcagctg gtccgtgtca
     2101 ccgctggcca cagcccgacc gccaccggcc gcaatcaggg cgtatgcccg tgccatgtcg
     2161 gtattgccca ccatagagct ctcatcgaga aggaacagcg tgttgctgaa atccggcgtt
     2221 tctccgctgc gctgctgcag ctgcatgtca tgcagaaagg acgccagtgt ctgtgcatcc
     2281 acgccggcgc tgcgcatctc cccgaccgca cggtgtgtgg gccccagccc cacgacacgg
     2341 ggacgtccac tctccggcag catgttcacg gctgacatca ccgcccggaa ctgtgtggtc
     2401 ttacccacac cggcatagcc ctgcaccacc gtgaaacggt cggacgtttc cagtatcata
     2461 cgggtggcgg cacgctgtcc tgatgtcagt ttctccatga gttcgccagg tactctctcc
     2521 atcagcggcg tgaccgcctc cttaccttcg agaatatggc gaagaatgct cttttctgcc
     2581 tcatacgacg cacgggaaac cagcaggcct gtgccatagc cttttgccac atccacatac
     2641 agtaaatcac cccgttttat ctgcgcattg atttcccctc ccaggtcagc aaaaccggtt
     2701 ccttctgcag caaacgactt tgcctctgtc agcaggtcca ccatgctgaa ggccaggttt
     2761 ttactttcca gcacaggaag ggccagatga atggcctgct gtgccggcgt gtgaagcccg
     2821 gctttctgca gactgatagc ggtttccagc aatgtctcac cggcacgcgc ctttatctgc
     2881 tcagaaacca ccgtaaagga gggatggcgg gcaagttttt ccgcagtacg ggtttcatcc
     2941 agtgaggaat acagccggac atcacgacca ctgcgggcca gaccgttcag ggtggcattg
     3001 tccattgcca tctgtgtgac ggaggcaaaa accgtcgcac tgtcgctgac ggaatgcccg
     3061 ggcgtttcca cccagccgtt ctccagcttc agtgccgtga acggtgaatc gctcacaggc
     3121 agggtggccg gttcagcccg tcccggcaca acaaccgtca tcgcatcttc actgacggat
     3181 gccacctgca ggcggtcacc gccggagacg cggagtccgg gaattttccc tgtcaccctc
     3241 agacgctcac cgtctgccac cggcattttt tccggccgga acagcgacca gctgctgtcc
     3301 agggaagaaa tacgcaccac ctgcgtttcg ccctgcgcat tccgcagggt caggctgtga
     3361 ctctgcgccg tcacacgttc tgtcacatag cggtcatgac tgcgtgtctc cgggttccac
     3421 tgctccatca ccattcccgg gcggtacata tcccgcagat aacggctccg gctgtccagc
     3481 cagaccggtg acagggcggt catggttacc tccgggtgtc cgagcacgcc ctgtgttttc
     3541 agctcactgc gaatggcctg tgtcagtatg gcctgttccc gtactccgct gacctgtgcc
     3601 acgctctctt ctccggcttt cacgctggcc gcaaaatctc cggccagccg ggcatagcgg
     3661 acataacggt ccggttcact gatgatggtg gccggtcgct gttctccccc ctgccagcga
     3721 tatgtgttca ccccggcatc cttcatggcc atcagtgcac tgcctgtacc ggttcgctgc
     3781 ccgctgtcag ttatcaggac ctgtacgtta tgacgtgcgg caccatccag cagggttaac
     3841 gtctctttca gggagagttt ttcgccctgg tcgacgataa cggtactgcc cggcgtgaag
     3901 gccatgcctt ccagcaactg acgacgtccg gttatcagtt caccggacag ccgttcatcc
     3961 tgcttcaggt tcatctgcga gcgacggtca gcagcgataa tctgcacctc ccgcccctgc
     4021 tcccgggcca tcatgaccag ttcagccacc cgctcacgct gcccggctgc accgccctgc
     4081 ccggacacaa tggccagcga cggacggtcc tgtgccagaa cactgacggc atcgctgtaa
     4141 ccggccgtcc gggggacact tttctccgga tgtacggtca cccggttctg tttcatgatg
     4201 tcacgactga gtgcccggac tgacagctca tcgagcatat gaatcccgga cgtgaacagc
     4261 cccttctcac gatcgagggg gataagctgc tcacggctga tggcctcatc gataccggcg
     4321 cgtgcccgtt caatcacacc attttccggc ggcagtatgc cgaccgtcct ggccagcagg
     4381 tccgtataca taaactgcac tttgcgttca cttaatccgg caatggcctg tgtcaccgcc
     4441 tgctgcacat ccggcccgtc ctgtgaagcg ggtccgggtg tctgcgtgcg ggtatatgcc
     4501 cgttgttccg ctgcgtcacg atatgcccgg atgtcgaacc cggtttcctt cagcgtctgc
     4561 atccactcgg ccattctgac ttcaggatcg acgtgctgtt tggatttacg cgtatccagc
     4621 gccgccacat cccgggattt cagcgaggcg tcttccccga cggcctcccg gatagtctgt
     4681 gagcgtccgg aaaaggcctc caccggtaca cccggcattt cccacatacc gtgcttaccg
     4741 accacttcag tttcatagcc cagcgcctca acctgctctt tcagtttttc ccggtagagc
     4801 ctgccaaagg caatctgatt agcgtacaca ttctcaatga accccgtttt ccccactttg
     4861 tcactgctca gtgtcttcca ttcgccgtta tgctgcgtaa cattagtcac caccgcatgc
     4921 gtgtgtaact gtggttcctg atcgcgactg gtgtcgtggt taaacagtgc catcaccaga
     4981 ttaccggtca gcaccgtttc tgactgtccg tccgtcatca cccgtgtgga ggccagtgcc
     5041 tccacacggg tgatgacgga cagcaaaatc cacggcctgg ttatgtgctt caataagacg
     5101 tttgtccccc ccgagcatgg ccatcatgga gacacttttg ggagcggaga aggtcaggtc
     5161 gtagccggga cgatgcctgt tactgccatc ctgcatgcgg cttaaatccg ccccgtccgg
     5221 cagcctgccc tccagaaggc gggtaaagac atccttatcg acactgccct gcagccccag
     5281 ttgttcagcc ccctggccgg cccagcgttc tcccatgctg cccagcacat agtaattatc
     5341 cttgtcggta taataattac cggcacttcc ggccgatctg acctgcgcga tactcatcat
     5401 aaacgtatat ccctttgaca tatcccggta tcaatcccac aatggatata cacaagacat
     5461 atccacataa ggaggcaaat aatggaaacc accgtatttc tcagcaaccg cagccaggcg
     5521 gtcagactgc caaaagcggt tgcattgccg gaaaacgtaa aacgcgttga agtgattgct
     5581 gtcggacgaa ccagaatcat tacgccagcc ggagagacat gggacgaatg gttcgacgga
     5641 cacagcgtca gcgccgattt tatggataac agggaacagc ccggcatgca ggaaagggag
     5701 tcattctgat gctgaagttt atgctcgata ccaacatctg catttttacg ataaagaaca
     5761 aacccgccag cgtcagggag cgttttaacc tgaaccaggg gagaatgtgt atcagttcgg
     5821 tcaccctgat ggagctgata tatgatgcag aaaaaagcca gatgcctgaa cgtaatctcg
     5881 ctgtgatcga gggatttgtt tcccgcattg atgttctgga ttacgacgct gctgcagcca
     5941 cacacaccgg ccagataaga gcagaacttg cccgtcaggg acgccctgtc gggccatttg
     6001 atcaaatgat cgcaggtcat gcccgcagtc gggggctgat tattgtgact aataacaccc
     6061 gggaatttga acgtgtgggc ggcctgagaa ctgaagactg gagctgacct gttcatcaga
     6121 aatcatctcc cggctcaaca tcctccccgc gttcccggtg cacattgatg ttcacctctt
     6181 cacgacgctg catctgctgc caggtgtccg gatgattttc ctgctgccat gcctcataag
     6241 cggccatatc caccacttca ccggattcac tgatcccggg tggcagttgc tgttccatct
     6301 cttcttccgg tttcattttc agctcctgct cgatcccccc tgccggaaca ttcacacctg
     6361 aatctgactt cttatcgttg atgacaggag acaccggctg ttgcggctgt tgcggctgtt
     6421 gcggctgttg cggctgttca gcctgagtca cgtcttctcc ggaaacaacc tccgggacat
     6481 ccggttcgaa gaggctggcc atctgacgac cttctgcttc ccttgcggca agtacggcac
     6541 tcagacggtt ctccatttcc gggttgatgt cacgcggaat aaactccgga gcgaccttcg
     6601 gtcgttcctg atatttcagt gagagtttga ctgccggata cggtccgggc agggtgacat
     6661 agcaggtcag atccggcagg gactgaatgt cggaataact gaccagcgtc tggcgctcca
     6721 tatccttacc ggtcgatacc ccgtcacgta ccggatcagc accgtaggaa tactgttcgc
     6781 tggctttcag gtgctctttc tcaccaattt caccggcagc gaactccgca atcttatggc
     6841 tgggagaacg gaaaaaggca cgggtgttca tgacgtcaaa cagcgtggca gccgctttct
     6901 caccgtagat atcttccagc tgggcatagg actggatacc aaacacataa cagccaccga
     6961 acttacgggc ttccggcagg atctccacca ggtccggcag tttgtataat gtgggtaact
     7021 catcacagaa gaaccacaca cgacgattac ggttttctcc cattgccagc agaccacgaa
     7081 tggcaatgga cagccacatg gagatcaccg gcttcaggga ggcatgagtg tcggcattcg
     7141 acgagataaa cagccagccg tttttctgat cctcccggac accacgcatc cagtcacgga
     7201 tggtgaaggg ctcaccgtta tgctcaatcc cctgcaggta acggatggct ttcacatagt
     7261 tggtcagcac agcacggatg gatatggccg ttttctcgat tttctcttcc accaggttgg
     7321 ccgccggtga attacgcagg taggtacgca gtttttcaat tttgatggaa agcagtgtgt
     7381 ccaccagttt gctgtagctg cggttggggt cattacgcat cagatacgcc gcttccgcaa
     7441 aaatggtacg tcctgaaccc tgccagaacg ggtcctcttt tgtgcccatc gggatcagag
     7501 tatttgcggt attatcaaaa tccggctgtg tcaggcactc cttccacaaa tcccaggcgg
     7561 cacagcgtgc atccagcgga ttcaggatct tatcgatgga gggatcatag taacttttaa
     7621 caaattcccc tgaacggtca taaatcacca ccatatcacc acgctgacgg gcgtagttgg
     7681 ccagacgacg gataacctcc gacttaccgg ccccgaccgt gccgtgcaga cagaagttct
     7741 ggatttcgga atcccggata atcggcagat cgccaatccg gatatcggag tccctgccgt
     7801 cttttttcag catccgggca acgtctttcg gattgtctgt cagttgacga ccacctgtga
     7861 cttcattttc gctctgttgt ttaccctgac gccccagaat ccaggagaca acaaagaagg
     7921 taatcaggca aataaccagt gccacaactg tggccagaac gaatgcagac cataactgct
     7981 caccgcacca gatcatatat ttatcatgca gtacctgagc agcgttcatc cggaaggttt
     8041 tgccgtaata ctgaatctca tataccggct gagacttgat taaatcccgc atgccttcca
     8101 gcgtggtaca ccaccagtaa atacagccat tcacaaacgt ctgccagctt atttttaccc
     8161 ataaaaccag accaacgagt atccagaaaa aaataaacag gcagtaaagc ataatattgg
     8221 cgatctggct gaacatgcgg atacgcatgg acgtaatctg accgccctgg gttatatcct
     8281 ttgcgttaaa actcatgatg atattccgaa gaaagaaagt cagtcggact gattattaat
     8341 gatgaaaaga ttaataaccg ttaaattccg gcttcgccgg caacacttca cggtcgcaca
     8401 aagaatttgc acaaccgctc agtgttttca tatttatttc ggaaacgccg gggagaaata
     8461 aaatttattt tatttctttc cgg
//
LOCUS       DAJTNA010000161         8481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTNA010000161 DAJTNA010000000
VERSION     DAJTNA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.163"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            196..906
                     /locus_tag="OO161_003234"
     CDS             196..906
                     /locus_tag="OO161_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5464203.1"
                     /translation="MATGKSFSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(956..1930)
                     /gene="ghrB"
                     /locus_tag="OO161_003235"
     CDS             complement(956..1930)
                     /gene="ghrB"
                     /locus_tag="OO161_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5464204.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIVPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(2034..2621)
                     /locus_tag="OO161_003236"
     CDS             complement(2034..2621)
                     /locus_tag="OO161_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5464205.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDNIILNMPNNVTFDSSSATLKPA
                     GANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMRLSQQRADSVASALITQGVDAS
                     RIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            2774..5107
                     /gene="bisC"
                     /locus_tag="OO161_003237"
                     /pseudo
     CDS             2774..5107
                     /gene="bisC"
                     /locus_tag="OO161_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
     gene            complement(5076..5516)
                     /locus_tag="OO161_003238"
     CDS             complement(5076..5516)
                     /locus_tag="OO161_003238"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5464206.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(5513..6076)
                     /gene="tag"
                     /locus_tag="OO161_003239"
     CDS             complement(5513..6076)
                     /gene="tag"
                     /locus_tag="OO161_003239"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5464207.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFYQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            6234..6932
                     /locus_tag="OO161_003240"
     CDS             6234..6932
                     /locus_tag="OO161_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5464208.1"
                     /translation="MIIKKSGGRWQLCLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRIGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            7160..8362
                     /locus_tag="OO161_003241"
     CDS             7160..8362
                     /locus_tag="OO161_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5464209.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         1979 a         2077 c         2235 g         2190 t
ORIGIN      
        1 tcgggctaca aaaaagcaac ggcaatatca ttttttaaca atgaaacaac aaattaacct
       61 tgtttcattt tctccatgat tttaccagga agattacgta gaaagggtat cctgcaagcc
      121 tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct ttttttgggt
      181 gaaaggagta agaaaatggc gacaggaaag tccttctctc gctggtttgc gcctcttgcg
      241 gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga tcagcgtaaa
      301 gcgtttatcg acttcctgca gaatacagtg atgcgtagcg gtgaacgtct accaaccctg
      361 actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat tctgtatggt
      421 tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggtcgt agacagcgtt
      481 aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg tgaaatgaac
      541 ggttcgctgg gtgtgctggc gcaacaactg caaaatgcga agttgcaggc cgatgctgcg
      601 cactctgcgc taaaacagac cgatgatttg aaaccggtct ttgatcaggc attcaccaaa
      661 gtggtaacga cgccagctga tgcattgcaa ccgttgattc cggttgcaca aactttcacg
      721 caacaactgg tgatggttgg agactacatt gctcaacaag gtactcaggt gagctttgta
      781 gcaaatggta tccagttccc gacttcgcag caggcaagtg aatataacaa actgattgcg
      841 ccgttaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt tactgcaacc
      901 caataaagag taaaagcccg agcgattatc gggctttact tcacgcagtc gcggcttagt
      961 ccgcgacgtg cggattcaca cagttcttct caacctttcc ttgtaacgca tcaatcaaat
     1021 tatccacggc acaggcggcc atgccataac gcgtctcatg ggtggcagat ccaatatgcg
     1081 gtactgcgac gacgttggcc attgagagca acggcgaatc tacggacagt ggctcttgtt
     1141 cgaagacatc cagcccggcg gcgtgaattt cgcctttctg caatgctgcg atcagtgcat
     1201 tttcgtcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat ttcattttgg
     1261 cgaattgttc tgcgccaaac agatgatgcg tctcatcagt taacggcagg atcaggcaaa
     1321 cgaaatctga ctcttgtaac agagtatcca aatcgcagta gcgggcgttg aagcgttctt
     1381 ctgcttcttt atggtggcgg cgcgcgttat agaggatggg catgttgaag ccaaagtgcg
     1441 cacgttgtgc cagcgccatg ccgatccgtc ccatcccgac aatgcccagt gttttatggt
     1501 gaacgtcagt gccgtaccag tccgggacta tgctcgcggt ccattcgcct gcttttaccc
     1561 gttctgctac ctccacaacc cgacgagcgg tagacaacac cagcgccatc agcgtatcgg
     1621 cgacggtttc tgttaatacg gttggcgtgt gcatcagcag aatttttcgg gcggtaagcg
     1681 catcgacatc aaaattgtca tagccgacgg agatcgttga tgtggcacgc agtttcggca
     1741 ttttttccag caatgcggca tctacattct cgtttgaacc cagtaaacct tcagcttcgg
     1801 caaaaattgc tgcattttgt tcgacggttt gtgggctgag gtttgccacc tggtgaacgg
     1861 tgaaatgctc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag aggataacgg
     1921 acggcttcat gcttctctcc attcactgat aaggcgctca ccttagcctg aaatatgatg
     1981 aaggaaaact gaagaatgcc aggtgatttc tgcatcacct ggcatgaaag ggattacagc
     2041 gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcggt gctgttgctg
     2101 gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc cacgccctgg
     2161 gtgatcaacg cgctggcaac ggaatccgca cgctgctggg agagacgcat gttcaggtcg
     2221 tgaccccccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg atactctttc
     2281 agcaccattg ccacgccggt cagggtgtta gcgcctgccg gtttcagggt cgcgctgctg
     2341 ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct gcgggttacg
     2401 ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc catgtaataa
     2461 cccacgccgc cgcccagagc tgcgcccacg agagagtcca gacctgcgcc gatagcagat
     2521 ttgcctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac cgccagagcg
     2581 ccactcacta cggcggcaat aagataaaca cgtttcttca ttgttaatcc ttaataacct
     2641 ttttattctt tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca tgaagattat
     2701 tcctgggaat actcggaaat ttgtaagtaa tatttaactg ctcaatacat ctaacctttc
     2761 aggagtcttc ggtttggcca actcatcctc acgatattcc gttctgactg ccgcccactg
     2821 ggggcccatg ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg gcgcgttagc
     2881 cacaggaatg gaaaactcct tgcagagcgc ggttcgcgac caggttcaca gcaatacgcg
     2941 ggtacgattt ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc cgcaaggcat
     3001 tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc ttattcacca
     3061 acaacataaa cgcattcgtg aggcttatgg tccggcatcg atttttgctg gttcctacgg
     3121 ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct atatggcgct
     3181 ggcaggcggt tataccgggc atctggggga ttattcgacc ggtgcggcac aggcgatcat
     3241 gcagtatgtc gtgggtggca gcgaagttta tcaacagcaa accagttggc cgctggtgct
     3301 ggaacatagc gatgtcgtgg tgttgtggag tgctaaccca ctcaatacgc tgaaaattgc
     3361 gtggaatgca tctgatgagc aggggctttc ttacttttcc gcactgcgtg acagcgggaa
     3421 aaagctgatc tgtattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat
     3481 ggagtgggtg gcaccgcaca tgggcaccga tgttgcgctg atgctgggga tcgcccatac
     3541 gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca caggttatgc
     3601 cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gccaaaaccg ccgaatgggc
     3661 ggcagggatt tgtggggttc acgcagcgaa aattcgcgag ttggcggctc ttttccacca
     3721 aaacacgacc atgctgatgg ctggctgggg aatgcagcgc caacagtttg gtgagcaaaa
     3781 acactggatg atcgtcacgc tggcggcaat gttggggcaa atcggcacac ccggcggcgg
     3841 ttttggtctt tcttatcatt ttgccaatgg tggtaatccc acgcggcgtt ctgcggtgct
     3901 ctcttccatg cagggtagct tgccgggtgg cagcgatgcg gtagataaaa tccctgttgc
     3961 ccgtattgtt gaagcactgg aaaaccctgg ctgcgcatac caacacaatg gcatgaatcg
     4021 cctcttcccg gatattcgct ttatctggtg ggcgggtggc gccaacttca cccatcatca
     4081 ggacactaat cgcctgatac gagcttggca aaaaccggag ctggtagtta tctccgaatg
     4141 cttctggacg gcggcggcaa aacacgcgga tatcgttctg cctgcgacta cctcttttga
     4201 gcgtaatgat ctcaccatga ccggcgatta cagtaatcag catctggtgc cgatgaagca
     4261 agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttaagtga
     4321 acgctgggag aagggcggtt atgcacgttt tacggaagga aaaagtgagt tgcaatggct
     4381 ggaaacgttt tataacgttg cccgacagcg cggggcaagc cagcaggttg aattgccgcc
     4441 atttgctgag ttctggcaag ccaatcagtt aattgagatg ccggaaaacc cggacagcga
     4501 gcggtttatt cgcttcgctg atttttgccg cgatccgcag gcgcatcaat taaaaaccgc
     4561 cagcggtaag attgaaatct tctcgcagcg tattgccgat tacgcttatc cggattgccc
     4621 ggggcatcca atgtggctgg agccggacga atagcagggc aatgccgaac cggaacagtt
     4681 acaggtactt tctgctcatc cggcgcatcg tctgcacagc cagctgaatt acagttctct
     4741 gcgcgaattg tatgcggtgg caaatcgtga gcctgtcacc attcatcctg acgatgccct
     4801 ggcgcgcggc attcaagatg gcgatactgt tcggctgtgg aacgcacgcg ggcaaattct
     4861 tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgtattc acgaaggggc
     4921 atggccggat ttggatttaa ccgctgacgg tatttgtaaa aacggcgcag tcaacgtgct
     4981 gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata cggcgctggc
     5041 atggctggaa aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag
     5101 ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa
     5161 ccctgcgcct ggtaaaaatt tatcgccggt tgattttttt gataaacctc cagcatcagg
     5221 tggggatggc gctgctgcac atattgcatc agcgccttac caataccgcg cctgacggcc
     5281 ttcggtgcga caaacatcgc tgccagaaat cggccttcca taatgctgac aaaaccgaga
     5341 agcttactgt cttcttccca gacccagttt tgcgcattgg caagatacgc atcccgcacc
     5401 agcggaatgc aatcacgcca gtaattcgct tttataaagg gatgtcccca ggttgtactt
     5461 tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt
     5521 tatttcccgg atagcaacag cagccaacca catgatcatt caccagccca catgcctgca
     5581 taaaggagta acagattgtg gtaccgacaa acttaaaacc acgttttttc agtgctttag
     5641 atagggcgtc ggaggcgggc gtagatgtgg ggatttcgct caacgttgtg gcttgtgtca
     5701 cctgtggctg atgatttaca aacgaccaga caaagtcggc aaacggttcg ccgttctgtt
     5761 ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttcccg cgatggcgga
     5821 taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgct gcgactttca
     5881 ccggatcgaa ctgataaaag caggcgcgat agttttcgcg ttttttgagg acggtgatcc
     5941 acgataaccc agcctgctgc ccttcaaggc agatcatttc gaacagtttt ttactgtcag
     6001 tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggtcc tgactcaccc
     6061 agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt
     6121 ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct
     6181 tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca
     6241 taaaaaaaag cggtggtcgc tggcagctat gcctgctggc gagcgtggta atcagtgcct
     6301 tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg caacccggac
     6361 attccacaga acgttacacc tgggatagtg atcatcaacc tgattacaac gatattttgt
     6421 cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa
     6481 ctcctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg
     6541 ttacaaccgg cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg
     6601 agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtagct
     6661 taggctggcg tgttgactcc cggattggcg atctccgacc ctgggcgcaa atcagctata
     6721 accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga
     6781 caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa
     6841 atattgccgc ctatgccgcg ctaactcagg cagaaaatac cactaataat agcgactatc
     6901 tgtatacgat gggggttagc gccagatttt aacgtaacag tcacaattga aaccattgaa
     6961 taacaatagt tgtgctgata gtgggtgcta acttacaaat aataaatttg gtgaataatt
     7021 gtcgcgtcat tcattcctga actaaggcat ttcattccgt tctgatggca tttcatgccg
     7081 tttttcccca ggcataaaat gcacttcgtt atggttgtcg gcagagattt ttccttttta
     7141 ttactgcagg aatactgcca tgacaccttc aaattatcag cgtacccgct ggctgacact
     7201 catcggtact atcattaccc agtttgcgct ggggtcggtt tatacctgga gcctgtttaa
     7261 tggcgcgctt tctgccaagc tggatgcgcc ggtaagccag gtcgctttct ctttcggctt
     7321 gttaagtctg gggctggcaa tttcatcttc tgttgcgggg aaattacagg aacgttttgg
     7381 cgttaaacgc gtcactatgg cttccggcat tttgctagga ttaggcttct tcctgacagc
     7441 gcattctgac aacctgatga tgctgtggtt aagcgccggt gtgctggtcg gtctggcgga
     7501 tggcgcgggt tacctgctga cgctctctaa ctgcgtgaag tggttcccgg agcgtaaagg
     7561 tctgatctcc gcgttcgcta tcggttctta tggtcttggt agcctgggtt tcaaatttat
     7621 cgacacgcag ctgctggaaa cggtgggtct ggaaaaaaca tttgtgattt ggggagcgat
     7681 tgcgctggtg atgattgttt tcggcgcaac gttaatgaaa gacgcaccaa aacaggaagt
     7741 gaaaaccagc aatggtgtgg tggagaaaga ttacacgctg gcagagtcga tgcgtaaacc
     7801 gcagtactgg atgttagcgg taatgttcct gactgcctgc atgagcggcc tgtacgtgat
     7861 tggggtagcg aaagatatcg cccaaagtct ggcacacctt gatgtggttt ccgcagccaa
     7921 tgcagtaacg gttatttcca tcgctaacct ttcaggtcgt ctggtgctgg gcattctgtc
     7981 tgacaaaatt gcccgtatcc gtgttatcac cattggtcag gtgatttcgc tggtgggtat
     8041 ggcggccctg ctgtttgcac cattaaatgc agtgacgttc tttgcagcga ttgcctgcgt
     8101 ggcatttaac tttggcggca ctattaccgt ctttccatca ctggtcagtg agttcttcgg
     8161 cctcaataac ctggcgaaaa actacggtgt gatttacctc ggtttcggta tcggtagcat
     8221 ttttggttcg attatcgcct cactgtttgg cggtttctat gtgaccttct acgtgatttt
     8281 cgccctgctg attctgtcat tggcgctttc tacgacgatt cgtcagccag agcagaaaat
     8341 gttgcgtgag gcgcatattt agaaatatgt tgctactgaa ataaatacga ggagggagac
     8401 ttcaaagtct ccctttttta tggacgcata ttaaaagaac aataatattc ataccacaaa
     8461 tatattttat ttatatatta t
//
LOCUS       DAJTNA010000162         8349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTNA010000162 DAJTNA010000000
VERSION     DAJTNA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.164"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..255
                     /locus_tag="OO161_003242"
     CDS             <1..255
                     /locus_tag="OO161_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5464210.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO161_003243"
     CDS             complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO161_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5464211.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO161_003244"
     CDS             complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO161_003244"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5464212.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2907..3182)
                     /locus_tag="OO161_003245"
     CDS             complement(2907..3182)
                     /locus_tag="OO161_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5464213.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO161_003246"
     CDS             complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO161_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5464214.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAQLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4423..5142
                     /gene="trmB"
                     /locus_tag="OO161_003247"
     CDS             4423..5142
                     /gene="trmB"
                     /locus_tag="OO161_003247"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5464215.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5142..5468
                     /locus_tag="OO161_003248"
     CDS             5142..5468
                     /locus_tag="OO161_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5464216.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5652..6371
                     /locus_tag="OO161_003249"
     CDS             5652..6371
                     /locus_tag="OO161_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5464217.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6547..7592
                     /gene="ansB"
                     /locus_tag="OO161_003250"
                     /pseudo
     CDS             6547..7592
                     /gene="ansB"
                     /locus_tag="OO161_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
     gene            7708..>8349
                     /locus_tag="OO161_003251"
     CDS             7708..>8349
                     /locus_tag="OO161_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5464218.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKAFDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRD"
BASE COUNT         2165 a         2022 c         2206 g         1956 t
ORIGIN      
        1 attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa
       61 ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg
      121 gcggttcaac gttatttcct tgcactggaa gaaggggtaa atttgttgcc gggattttcg
      181 ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt
      241 tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc
      301 gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca
      361 tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac catacggtct
      421 gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc
      481 cgaggataca gccgaaaccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa
      541 ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg
      601 cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc
      661 cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct
      721 taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct
      781 gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca aacatcggat
      841 ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc
      901 ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac agcgcgaatg
      961 catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat
     1021 gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct
     1081 gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga
     1141 tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca
     1201 ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa
     1261 tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg aacagcgtga
     1321 tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca
     1381 caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta taaactgcgc
     1441 caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc
     1501 aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca
     1561 tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgagcg
     1621 agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg
     1681 caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca
     1741 tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt
     1801 tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat
     1861 cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc
     1921 gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc cgcgatgttg
     1981 ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg gtgccggtat
     2041 caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg
     2101 agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc agcgcatcgg
     2161 aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc agaatcagcg
     2221 actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac
     2281 gtttatcaag gtggttcggt accatgttaa tggtgacgct gtagatgata cgcagcccgt
     2341 tactgcggct ttgcagacgg tttttcagca gataatccgc gaagttgctt gcgcgacctt
     2401 cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg
     2461 aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg tcacccatca
     2521 gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg gcgatagttt
     2581 cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt
     2641 atttcacgta gtccttagga ccggcgatca ccacctcttt gaatccccag atgttctcaa
     2701 tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg acccaggctt
     2761 cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag
     2821 ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt
     2881 gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata gccctcgata
     2941 tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag cttgcggtgc
     3001 tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt gtgctgccac
     3061 tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc gccggggtac
     3121 agctgaaaat cctgaccttc tgcttcacgt tgcaggaaag tacaaaaaat cgttctgctc
     3181 ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg taactgctgt
     3241 aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa gttataccag
     3301 agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca cataggcaca
     3361 atatctaagt ggaaatggct gaaggtatgc cgaaacgcgg tcagttgcgt caggttatcg
     3421 gcagcaatct gccgttgcgc cagccactgc cgcaaacttt cttcgtcggc aaactgcggg
     3481 aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa tacttcatct
     3541 tcgtgctgta acagcaaaaa gtagccggtg cgctccggca gcgtctgttt cggttttttg
     3601 cccggataaa gcgaccagct attattggcg gcggcaatac atccgttttg tagcggacag
     3661 agcgaacatt tcggcttcga gcgcgtacaa atcatcgcgc ccaaatccat catcgcctga
     3721 ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca taatttattc
     3781 tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag cacgcgtttg
     3841 acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg
     3901 gtggaacgcc cgacgcccgg taacgccgcg acttcttcaa aggtttccgg gaatttaccg
     3961 ccgtgtaagg tcgccacttg ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag
     4021 ccaagcccgg tccacaagtg gagaacttcg tcgagctgcg cattggcgag atcggtcacc
     4081 gtcgggaagc gcgccataaa gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt
     4141 tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg ccaaggcagc
     4201 gtttttcgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc
     4261 atggtcaccg aattcactgt tgttgggggc aagattgcag cacagcgacg gcagggtgta
     4321 aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact aactttggat
     4381 aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt
     4441 tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc
     4501 caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc ggtgatgggc
     4561 gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga agcgccggtg
     4621 acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa agatcgccct
     4681 gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct
     4741 gcgcatgaag aaggtttaag caacctgcga gtgatgtgtc acgatgcggt tgaagtgctg
     4801 cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc tgacccgtgg
     4861 cacaaagcgc gccataataa acgccgtatc gtacaggtgc cgtttgccga actggtaaaa
     4921 agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc ttatgcggaa
     4981 catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga gagcaatgat
     5041 tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt
     5101 ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag aaccgtagcc
     5161 gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt tcggtggcat
     5221 ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat gattttatta
     5281 acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg gcctgggaag
     5341 gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg attgtgcgta
     5401 agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc gacgtttggt
     5461 gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat
     5521 caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc gcatccggca
     5581 tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg attttttctt
     5641 gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg acggcaatga
     5701 ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc
     5761 aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac ggcaacgtga
     5821 tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg aaggattatc
     5881 aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaaa
     5941 aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgaaagc agcaaaatgc
     6001 gcagccgtct gaccaaactt gatgcgcagc tgaaagagca gatgaaccgc attatcgaaa
     6061 cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc gccgaaggcc
     6121 agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat gaaatgggcg
     6181 cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga agcctggggg
     6241 ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc cagcagtttg
     6301 gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc ctggtcggga
     6361 atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt ttaattcttc
     6421 gttttgttac ctgcctctaa ctttgtagat ctccaaaata tattcacgtt gtaaattgtt
     6481 taacgtcaaa tttcccatac agagctaagg gataatgcgt agcgttcacg taactggagg
     6541 aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg ttttagtggt
     6601 gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat tgccggtggt
     6661 ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga aaatctggtt
     6721 aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt agtgaatatc
     6781 ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat taacaccgac
     6841 tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga agaaaccgct
     6901 tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgc
     6961 ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt agtgaccgag
     7021 ctgataaagc atccgctaat cgtggcgtgc tggtggtgat gaacgacacc gtactggacg
     7081 gtcgcgatgt aaccaaaacc aacaccaccg acgtagcgac cttcaagtct gttaactacg
     7141 gtcctctggg atacattcac aacggtaaga ttgactacca acgtaccccg gcacgtaagc
     7201 acaccagcga tacgccattc gatgtctcta agctgaatga gctgccgaaa gtcggcatcg
     7261 tttataacta cgctaacgca tccgatcttc cggctaaagc actggtagat gcgggctatg
     7321 atggcatcgt tagcgctggt gtgggtaatg gtaacctgta taaatccgtg ttcgacaccc
     7381 tggcaaccgc cgcgaaaaac ggcactgcag tagtgcgttc ttcccgcgta ccgacgggtg
     7441 ctaccactca ggatgctgaa gtggatgatg cgaaatacgg cttcgtcgcc tctggcacgc
     7501 tgaacccgca aaaagcgcgc gtcctgctgc agctggctct gacgcaaacc aaagatccgc
     7561 agcagatcca gcagatcttc aatcagtact aatcacctcg ccccggtatc gtgccggggc
     7621 tttttcactt cagacttacg tccattgcca attttcatta ccctagtgat aatcacgaaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggct atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattatcgc
     8161 tttagtcagc ttgaatctgc gttgaatacc cagaagaact cgattcctgc gctggaaaag
     8221 gaagtgaaag cgtttgataa gcaaatggtg gcggcgcaga aagcagcgga tgcatattgg
     8281 ggaaaagatg caaacggtaa gcaaatgacc cgcgaagaag cctttaaaaa aattcaccaa
     8341 cagcgtgat
//
LOCUS       DAJTNA010000163         8349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTNA010000163 DAJTNA010000000
VERSION     DAJTNA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.165"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..971)
                     /locus_tag="OO161_003252"
     CDS             complement(<1..971)
                     /locus_tag="OO161_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5464219.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSAEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            1063..1149
                     /locus_tag="OO161_003253"
                     /pseudo
     CDS             1063..1149
                     /locus_tag="OO161_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1450..1539
                     /locus_tag="OO161_003254"
                     /pseudo
     CDS             1450..1539
                     /locus_tag="OO161_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            1563..6077
                     /locus_tag="OO161_003255"
                     /pseudo
     CDS             1563..6077
                     /locus_tag="OO161_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            6078..7727
                     /locus_tag="OO161_003256"
     CDS             6078..7727
                     /locus_tag="OO161_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5464220.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            7732..>8349
                     /locus_tag="OO161_003257"
     CDS             7732..>8349
                     /locus_tag="OO161_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464221.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQV"
BASE COUNT         2032 a         2002 c         2346 g         1969 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatccgtgg gccgtggtag tccggcgaag aggttaatct ttgcagggct
      181 tgcagagtgc gagtcgacaa tggattgtta taaatattaa catctgcata aatgtgcaga
      241 ttcatgatac tgtctggcaa tgtgttcagc tgattgttag ggatatatag tgattgcaga
      301 cgttgtggaa gttcgggcag tctgtgtagt tggttttccc caacgtttaa aagcctcaga
      361 ttttgaggaa ggggcggtag ctcttgtaat ctgttatcaa aagcacttag ttcctgtagt
      421 tcacatggta attctggtaa ggcagtaagc tggttgttgc caacagaaag tttttgtaga
      481 gccgggggaa gatcaggtag cgtctccagg ctattgagaa gggctgagag tgactgcaag
      541 gaagatggaa gagaaggtaa acatgttaaa actctgttaa gtgaaacatc aagagcgacc
      601 aggtgaggcg gaagagccgg gagcctgctc agtctgttat cgctggcttt aagtacagtt
      661 aaggacggag ggagttctgg cagagagcgt agctcattgc tagagatgtt aagttcttgt
      721 atgtgcgggg gcaggtatgg gagagagcgt aatctgagta aacttaaatt gagggctggc
      781 tcttgaaaag ccagacatat tttcagtaat cgaactgcct gtgttcggtc ttctgttgca
      841 gcaccttcct tggcccagtt atcccagatg cggtgataat ccgtgatatt ttgctcttct
      901 gcggataaac gggatataca tcctaaacca caaaaggagc ggaaaaaaga aggattgtgg
      961 gcaggtttca tttattattg atcctcattg tttatatatt aatatgttcg tgcaaatgga
     1021 gtgaggattc tttaaaaaaa cgctcctgta aatagttaag aacaggaaga actgttgggg
     1081 atgtggctgg ccgaaaatga aggggtgaag ttctggctca atgtgctgac tgaactgaaa
     1141 aaacgcggtt ggtcgcccta ccttttcatc aagccagtag tctgcccacc actgcatcat
     1201 ttctctgcgc ttatcgagat actgagcatg gttgtaaatt caactaaccc agtgcgttga
     1261 caaaaaatta gcgcaagaag acaaaaaatc accttgcgct aatgctctgt atcaggtcac
     1321 taataccagc taagtagttg attcatagtg actgcatatg ttgtgctttg tcgcattttg
     1381 tagtctatta tttaatctgc aacaacgata atacaatgaa ttttaattgg tttttgtgat
     1441 tttgtccttc ttctgacatg gaaggacacc cgatggatac ccaacgattc caatcccaat
     1501 ttcattggca tttatcgttt aaattttctg gcgcgatagc agcgtgcttg tctctgtctc
     1561 ttgtgggaac agggcttgct aatgctgatg attcgcttcc ttccagtaac tatgcgccgc
     1621 ccgccggggg aacattcttt ttgcttgctg acagcagttt tagcagcagt gaagaggcga
     1681 aagtgcgact ggaagcgccg gggcgtgatt atcggcgcta tcagatggaa gagtacggcg
     1741 gcgtggacgt tcgcctgtat cgtattcctg acccgatggc atttttgcgc cagcagaaaa
     1801 acctgcatcg cattgtggtg caaccgcaat atctgggcga cgggctgaac aatacgctga
     1861 cctggctgtg ggataactgg tacggcaaat ctcgccgcgt aatgcagcgt actttctctt
     1921 ctcagtcacg gcagaatgtg actcaggcat tacccgaatt acatctcggc aatgccatta
     1981 ttaaaccttc ccgttatgta cagaacaacc agttttcccc gctgaaaaaa tatcccttgg
     2041 tggaacagtt ccgttatcca ctatggcagg ctaaaccggt cgagccgcag caaggggtaa
     2101 aactggaagg cgcatccagc aatttcatct cgccgcagcc gggtaacatt tatattcctc
     2161 tcggccaaca agagccggga ctgtacctcg tcgaggcgat ggttggtggg tatcgggcga
     2221 cgacggtggt gtttgtttcc gataccgtgg ctcttagcaa agtgtcaggc aacgagcttc
     2281 tggtgtggac cgcgggtaaa aaacagggtg aagcgaagcc cggctcagag atcttgtgga
     2341 ctgacggtct tggcgtgatg acccgcggtg tgaccgatga cagcggtacc ttgcagttac
     2401 aacatatatc gccagaacgt tcatacattc tgggtaagga tgctgaaggc ggcgtttttg
     2461 tctccgagaa cttcttctac gaaagcgaaa tctacaacac ccgcttgtat atttttaccg
     2521 atcgcccgct atatcgcgca ggcgatcgtg tcgatgttaa agtgatgggc cgcgagttcc
     2581 acgatccgtt gcattcatcc cccatcgtca gcgccccggc gaagctttcg gtgctggacg
     2641 ctaacggcag tctgttgcaa accgtcgatg tcacgctgga tgcgcgcaat ggcgggcagg
     2701 gaagtttccg cctgccagaa aatgccgttg ccggaggtta tgagttacgt cttgcttacc
     2761 gcaatcaggt ctatagcagc agttttcgcg tggcaaacta catcaagcca catttcgaga
     2821 ttggtttagc tctcgacaaa aaagagttca aaactggcga agcggtcagc ggcaaactgc
     2881 aactgctcta cccggatggc gagccggtaa aaaatgcccg cgtgcagtta agtttgcgcg
     2941 ctcagcaatt atcaatggtc ggtaacgatt tgcgttatgc cggacgtttc cccgtgtcgc
     3001 tggaaggcag cgaaacggtg tccgacgcca gcggtcatgt ggcgttaaat ctccccgccg
     3061 ccgataaacc gagccgttat ttgttaaccg tctccgccag tgacggcgcg gcgtatcgtg
     3121 tcaccaccac caaagagatc ctcattgaac gcggtctggc gcattactca ttaagtactg
     3181 ccgcacaata cagtaatagc ggcgagtcgg ttgtgttccg ttatgccgcg ctggaatctt
     3241 caaaacaggt tcctgttacg tatgaatggt tgcgtctcga agaccgcacg agccatagcg
     3301 gagagctacc gtcaggcggc aaatccttta ccgtcaattt cgctaaacct ggcaactaca
     3361 atctgacatt acgcgataaa gacggcttaa ttctcgctgg gttaagttat gccgtcagcg
     3421 gtaagggcag cacggcgcat acgggtacgg tagatatcgt ggcggataaa acgctgtacc
     3481 agccaggcga aaccgcgaag atgctgatta cctttccgga gccaattgat gaagcattat
     3541 tgacgctgga acgcgatcgc gtggaacagc agtcgctgct ttcgcatccg gcaaactggc
     3601 taacgctaca acgtttaaac gatacccagt atgaagcccg ggttccagtg agcaattcct
     3661 ttgcgcctaa catcactttt tcggtgctgt atacccgtaa tggtcagtac agttttcaga
     3721 acgccgggat caaagttgcc gttcctcagc ttgatatccg ggtgaaaacg gacaaaaccc
     3781 attaccagcc tggtgaactg gtcaatgtcg aattaacctc gtcgctgaaa ggtaaacctg
     3841 tttctgcaca gctaacggta ggcgtggtcg atgaaatgat ctacgcgctg caaccagaaa
     3901 tcgcgccgaa tatcggcaaa tttttctatc cgctggggcg taacaatgtg cgtaccagct
     3961 ccagtctgtc gtttatcagc tacgaccagg cgctctccag cgagccggtt gcgcctggcg
     4021 caactaaccg cagcgagcgg cgagtaaaaa tgcttgaacg tccacggcgt gaagaggtgg
     4081 ataccgcggc atggatgccg tcactcacaa ccgataaaca aggcaaagcg tatttcacgt
     4141 tcctgatgcc tgattcgtta acccgctggc gtatcaccgc gcgtgggatg aacggcgacg
     4201 ggctggtcgg gcaggggcgt gcttatctgc gttcggaaaa aaatctctac atgaagtgga
     4261 gtatgccaat ggtgtatcgc gtgggcgaca aaccggcggc aggactgttt atcttcagtc
     4321 agcaggataa cgaaccggtg gcgctggtga ctaaatttgc aggcgctgag atgcgccaga
     4381 cgctgacgct gcacaaaggg gcgaattata tttcgctggc gcagaatatt cagcaatctg
     4441 gcttgttaag tgcagaactg caacaaaatg ggcaagtgca ggacagcatt agcacaaaac
     4501 tgtcttttgt ggacaacagc aggcccgttg aacagcagaa aaatgtcatg ctcggcggtg
     4561 gcgagaacgc gctgatgttg cccgagcagg cgagtaatat ccggctacaa agtagtgaaa
     4621 cgccgcagga gattttccgc aacaatcttg atgcgttagt cgatgaaccg tggggtgggg
     4681 tgatcaacac cggtagccgt ctgatcccgc tcagtctcgc ctggcgttcg cttgccgctc
     4741 atcaaagtgc cgccgctaac gacattcgtc agatgattca ggataaccgt ctgcggctga
     4801 tgcaactggc ggggcccgga gcgcgcttta cctggtgggg tgaagatggc aatggtgacg
     4861 ccttccttac ggcatgggca tggtacgccg actggcaggc cagctaggcg ctcggcgtaa
     4921 cgcaacaacc ggaatactgg cagcatatgc tcgacagtta tgccgagcag gcggataaca
     4981 tgccgttatt gcatcgggcg ctggtgctgg cgtgggcaca ggagatgaat ctgccgtgca
     5041 aaacgttgtt gaaagggttg gatgaagcta tcgcccggcg cggaactaaa gatgaagatt
     5101 tctctgagga agacatccgc gatatcaatg acagcctgat cctcgataca ccggaatctc
     5161 cactggcaga tgcggtggca aacgtcttaa ccatgacgtt gctgaaaaaa gcgcagttga
     5221 agtccacggt gatgccacag gttcagcaat atgcgtggga taaagcggca aacagcaatc
     5281 agccgctggc gcacacggtt gtgctgctca atagcggtgg tgacgctacc cagacggccg
     5341 ctattttaag tggtttgacc gctgagcaat ccactattga gcgcgcgctg gccatgaact
     5401 ggctggcgaa atatatggcg acaatgcctc cagttgtttt gcctgcgcct gcgggcgcat
     5461 gggctaaaca taagttaact ggagggggcg aatactggcg ttgggttggt cagggcgtgc
     5521 cggacaatct ctcttttggt gacgaattat cgccgcaaaa tgtgcaggtc cgctggcgtg
     5581 agccggcaaa aatggctcaa caaagtaaca ttccggtgac cgttgaacgc cagttgtatc
     5641 ggcttatccc tggtgaagaa gagatgagct ttattctgca accggtgacc agcaatgaga
     5701 ttgacagcga tgcgctgtat ctcgatgaaa tcacgcttac cagcgagcag gatgcagttc
     5761 tgcgctacgg tcaggtggaa gtaccgctgc caccgggagc cgacgttgag cgcacaacat
     5821 ggggcatttc ggtcaataaa cccaacgccg cgaaacagca ggggcaattg ctggaaaaag
     5881 cgcgaaatga aatgggcgaa ctggcctata tggtgccggt gaaagaactg acgggaacgg
     5941 tcactttccg ccatttgctg cgcttctcgc aaaaagggca attcgttctg cctcctgctc
     6001 gttatgtgcg ttcctatgca cctgcacagc aaagtgttgc ggcagggagt gaatggaccg
     6061 ggatgcaggt gaaataagtg aactggcgca gaatcgtctg gctattggcg ttggttactc
     6121 agccaacgct tgcggaggag acgccgttac aactggcact gcgtggtgcg cagcacgatc
     6181 aactgtatca gctgtcgtca tcaggcgtga ccacagtctc agcattacct gactcgctca
     6241 ccacgccgtt agggagtttg tggaaactgt atgtttacgc ctggctggag gatacccatc
     6301 aaccggagca gccctatcag tgtcgcggaa actccccgga agaggtttat tgttgtcagg
     6361 ctggggagag cataacgcgc gataccgcgc tggtacgttc ctgtgggctc tattttgcgc
     6421 cgcagcggtt gcatattggc gcagatgtgt gggggcaata ctggcaacag cgtcaggcgc
     6481 ctgcgtggtt agcgtctctt actacgctga agccagaaac ctcggtaacg gttaaatcgc
     6541 tgcttgattc attagcgacg ttacctgcgc aaaacaaagc tcaggaagtc ttactggatg
     6601 tggtgctgga cgaggcgaaa atcggtgtcg catccatgct cggtagtcgg gtacgggtga
     6661 aaacctggag ctggtttgcc gatgataaac aggaaatccg tcagggtggt tttgccggct
     6721 ggttaaccga tggtacaccg ctgtgggtta ctggcagtgg caccagcaaa acggtactaa
     6781 cccgttatgc gacggtgctt aatcgggttt tgccagtgcc gacgcaggtt gccagtgggc
     6841 aatgtgtaga ggtggagctg tttgcccgct atccgctgaa gaaaatcaca gcggaaaaga
     6901 gtacgacggc agtaaaaccc ggcgtactta atgggcgcta tcgtgtgacg tttactaatg
     6961 gtaaccacat tacgtttgtc agccacggtg agacgacctt actgagcgaa aagggcaaac
     7021 tgaaattgca gtcgcatctc gatcgtgaag agtacgttgc cagagttctg gatcgcgagg
     7081 cgaaaagtac gccccctgag gcagcgaaag ccatgaccgt ggcgattcgc acttttttac
     7141 agcaaaacgc caatcgggaa ggcgattgcc tgaccatccc cgacagcagc gccacgcagc
     7201 gtgtttctgc ttcgccagcg accacggggg cgcgaacgat gaccgcctgg acgcaggatc
     7261 tcatctatgc aggcgatccg gttcattatc atggcagtcg agctaccgaa gggacacttt
     7321 cctggcggca agccacggcc caggcggggc agggagagcg ttacgaccag atactcgcgt
     7381 ttgcttatcc cgacaacagc cttagccgct ggggagcgcc acgctcaacc tgccagttat
     7441 tacccaaagc gaaagcctgg ctggcgaaaa aaatgccgca gtggcggcgt atattacaag
     7501 gcgagacggg gtacaacgaa ccagacgtgt ttgcggtctg tcgtttagtc tcaggtttcc
     7561 cctataccga tcggcagcag aaacggctgt ttattcgtaa cttctttacg cttcaggatc
     7621 ggctggattt aacccatgag tatctgcacc tggcctttga tggttatccc accgggcttg
     7681 atgagaacta tatcgaaacg ctgacccgac aattattaat ggactaatgc tatgcgaaag
     7741 atttttcttc cgttgttact ggtggcactt tcgcctgtcg ctcacagtga aggtgtgcag
     7801 gaagtcgaga ttgacgcacc gctctctggc tggcatccgg cagagggcga agatgccagt
     7861 ttttcacaat cgatcaatta cccggcgtcg tccgtcaaca tggcagatga tcaaaatatc
     7921 tcagcgcaga tccgcggcaa aataaagaat tatgctgcgg cgggtaaaat tcagcagggc
     7981 cggctggtgg tcaacggtgc cagtatgccg cagagagttg aatccgatgg ttcatttgca
     8041 cgcccttata ttttcactga aggcagcaac agcgtgcagg tcatcagccc ggatgggcaa
     8101 agccgacaaa aaatgcagtt ttactcaacg ccgggtacag gaacgattcg tgcacgttta
     8161 cggctggttc tctcgtggga tacggacaat accgatctcg atctccatgt tgtcacgccc
     8221 gatggcgaac acgcctggta cggtaacact gtgctgaaaa acagtggtgc actggatatg
     8281 gatgtcacga cggggtacgg acccgagatt ttcgccatgc cagcgccagt tcacggacgc
     8341 tatcaggtg
//
LOCUS       DAJTNA010000164         8281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTNA010000164 DAJTNA010000000
VERSION     DAJTNA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.166"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..421
                     /locus_tag="OO161_003258"
     CDS             <1..421
                     /locus_tag="OO161_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HCR5464222.1"
                     /translation="AEPTHSPCMLLGVHTGLHDQISVQDIGIDNLTAADGLAVGRASG
                     FVGRAMVRLLDGFYTLSDQAMYDMLGWLAQEEGIRLEPSALAGMAGPQRVCASVSYQQ
                     MHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(529..2067)
                     /gene="emrY"
                     /locus_tag="OO161_003259"
     CDS             complement(529..2067)
                     /gene="emrY"
                     /locus_tag="OO161_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5464223.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2067..3230)
                     /gene="emrK"
                     /locus_tag="OO161_003260"
     CDS             complement(2067..3230)
                     /gene="emrK"
                     /locus_tag="OO161_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5464224.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            3646..4260
                     /gene="evgA"
                     /locus_tag="OO161_003261"
     CDS             3646..4260
                     /gene="evgA"
                     /locus_tag="OO161_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5464225.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            4265..7858
                     /gene="evgS"
                     /locus_tag="OO161_003262"
     CDS             4265..7858
                     /gene="evgS"
                     /locus_tag="OO161_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5464226.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVDSPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEASITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKISMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPISDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(7914..>8281)
                     /locus_tag="OO161_003263"
     CDS             complement(7914..>8281)
                     /locus_tag="OO161_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HCR5464227.1"
                     /translation="ALCQALELTELVNDPRFSSNILRVQNQAILKQYIERTLKTQAAE
                     VWLARIHEVGVPVAPLLSVAEAIKLPQTQVRNMLIEAGGIMMPGNPIKISGCADPHVM
                     PGAATLDQHGEQIRQEFSS"
BASE COUNT         2502 a         1802 c         1674 g         2303 t
ORIGIN      
        1 tgccgaacca acgcactccc cttgtatgtt gttaggcgtc catacaggat tacacgatca
       61 gatttctgtt caggatattg gtatcgacaa ccttaccgca gcggatggcc ttgcagttgg
      121 tcgcgcatca ggctttgtcg ggcgggcaat ggtgcgtctg ctggatggct tctataccct
      181 tagcgatcaa gccatgtatg acatgcttgg ctggctagcg caggaagaag gtattcgtct
      241 tgaaccttcg gcactggcgg gtatggccgg acctcagcgc gtgtgtgcat cagtaagtta
      301 ccaacagatg cacggtttca gcgcagaaca actgcgtaat accactcatc tggtgtgggc
      361 gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa tatctggcaa aaggccgtta
      421 ataacgtttc aacgcagcat cgcaatcctt tccctgggtg agcgatgctg ccgatggcgc
      481 agacttaaga tccccggtct tacccgctat aaccccctcc ttttatcatc acccaacgcc
      541 tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc gtaagtaaga taaaagcaat
      601 agccgccata cggaaaatct catttgcaga aatagaaagt gactgctggg ttatttcatt
      661 attaatttca ttaagaactc ctgaaagcga accataatat ttatccataa tttgtgacga
      721 tgaattaaac acggggttaa attgatcgat ggttgctgtc aactgactat ggtgtaacga
      781 ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca actgatcctg acaaggtacg
      841 aaaaaaatta ctcatactcg aggcattggc aaatttatta tctggcaagc ctgaaaacga
      901 aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat ccctgaaaaa actgcggcaa
      961 aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca gaacgccagt aatagcaaac
     1021 cgcatacatc aaaaaactaa atgtcactaa caaccgcatg tctattttgt tgccataacg
     1081 tcctatcaaa ggtgaaatta atagtggcat gatgccgatg ggcgcataag caagtccggc
     1141 ccatatcgca ttatacccca tcgtttcctg gagtaactgc ggcataagga cgatcgctcc
     1201 agagtaaaat aaatacgcgc atgtgatact cacaatacca atggtgaagt tacgggactt
     1261 aaacaaactg agatcaagaa tcgggttctc tgaggtcgac tcccaaatga ctaaagagat
     1321 cagagaaata actgatacta ctgttaatat tattattgta ctcgagttga accaatccag
     1381 atcgcgccct ttatcaagca taatttgcaa gccaccaaca ccgagcacta acagggtcag
     1441 tcctggtaga ttcattttga ccggtgaagt ctcagtttct cttcctttaa gtaaggttaa
     1501 gcataatgtc aggacgataa tccccatagg gacattgatt aaaaatatcc aaccccagct
     1561 aaagttatca caaatataac cgcccaatat cggcccacat atcggagcga taatcacggt
     1621 cattgaccat aatgccagag caaatgttct tttttctggc ggataattcc ttaataataa
     1681 actctgtgac agtggaatta acggccccgc cattaacccc tgaacgactc taaaaaatat
     1741 cagcacatca agattggtcg ataggctaca cattaatgaa gacagcgaaa aaaaggttac
     1801 tgaaagtaaa aataatctta attcgcctat tctttgtgcc aacctgccag taacagggat
     1861 cgcaatggca tttgctacac caaacgaggt gataacccag gtgccttcgt ctgttgatgc
     1921 tcccagaaag ccagatattg tcggtattgc gacgttagaa atagtggaat ccaacatttg
     1981 cataaatgtc gctaatgaca atgcaatagt gacgcaccat aacgtcccac cggttaatgg
     2041 tgccggagtt gatttagtga ttgccattaa agttgtccat tatgcgaaat aatgttgcta
     2101 atttcttttt ctatcggact ggtatcgata actaaagcct tactggtata agccggcatg
     2161 gaggtcacgg ttgaagccag ctcaggcatc tcggcaatgt cttcgttctt cgtatcaata
     2221 gttgctgtca tcgataaacc aatacgcaag ggatgttcca tgagttcttt tggatcaaga
     2281 gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt tccctgtcgc attttgtgca
     2341 ggtaataagg agaacgcatt gccggttccc atattgatcc ctgtcacccg accatgaaac
     2401 acaacatttt caccataaag atcgctgata atattgaccg attgaccaat ccgcacatcc
     2461 gtgagttgtg tttctttaaa gttggcatta acccacattt gacgtgccgg tacgacagcc
     2521 attaacgatt gtccggggct cactgtttcg ccgacctgaa cacttctctg ggcaatatag
     2581 ccggtaaccg gactcttaat atccgtacgt ttaagcgcca accaggcttc tttagttgca
     2641 tccgccgctt caacgacttg tggctgacgg tttaatggtg tgttcattac taaagcttta
     2701 ttcgctttat aagcctggat agcggcattc aatgccgctt tgctacttat taacgtatct
     2761 ttggtatgct ccagcgtttc ttttgaaata accccctgct tcgctaacgg cactcgacgg
     2821 ttataatctt ctaaagattg ttgatactga atacgtgctg aagcgacttc ggcactgtat
     2881 tgtttatcct gtaagtatag tttattcgtt tgccgaacaa tatttgccag attattttta
     2941 gctttattga gtgcgatagt ggcatcagtt ttatccagtg aaactaaaat gtcaccttgt
     3001 cgaacgtagt tcgtatcttt atgattaacg acagtgacac tacctgagac ttgtgcagaa
     3061 attggatctg cattccccgt gacataggcg tcatctgtac taatcatgtc ttctaattcc
     3121 attgaccaat aggcataggc acctgaaaac gcaataaata aaactaccgc caataaagaa
     3181 aagtattttc ttctgttaga atgtttttta tttgaattaa tctgttccac tattatctct
     3241 catttctcat agatgaaatt tatgaattga atgaaaagag cattcattct tcatatcaaa
     3301 cacagtgcat gtgttgcaca ttatcccagg aacgttggag gcctaattta aatgaggtgc
     3361 aaaaaaaaca acagaggact aaaccgtggc ttttgcaata caattcttac gcctgtagga
     3421 ttagtaagaa gacttatagt gctaacttga aactataaat catcggtaca acccctgatt
     3481 ttattgttga catttcattt atgccgacta tttatatggt atacttgtcg aattatctta
     3541 aaggaagctc agattttctt atttttattg agaaaatgag atgacgcctt atgtctgtat
     3601 tactacaggg agaagggaga tgcttcattg caaagggaat aatctatgaa cgcaataatt
     3661 attgatgacc atcctcttgc tatcgcagca attcgtaatt tattgatcaa aaacgatatt
     3721 gaaatcttag cggagttgac tgaaggggga agcgccgttc agcgggtgga aacacttaag
     3781 cctgatatcg tcatcattga tgtcgatatc cccggagtta acggtatcca ggtgttagaa
     3841 acgctgagga agcgccaata tagcggaatt attattattg tctccgctaa aaatgaccat
     3901 ttttacggga aacattgtgc tgatgctggc gctaatggat tcgtgagtaa aaaagaaggc
     3961 atgaacaata tcattgcggc tattgaagct gcaaaaaatg gctactgcta tttccccttc
     4021 tctctcaacc ggtttgttgg aagtttaacg tccgaccagc aaaaactcga ctccttatcg
     4081 aaacaagaaa ttagtgtcat gcggtatatt cttgatggca aggataataa tgacattgct
     4141 gaaaaaatgt tcatcagcaa caaaactgtc agcacttata aaagtcgcct gatggaaaaa
     4201 ttagaatgta aatcactgat ggatctttac acattcgcgc aacgtaacaa aatcggctaa
     4261 tcacatgaag tttttaccct atttttttct tctctgttgt ggtctttggt cgaccataag
     4321 tttcgcagac gaagattaca tcgaatatcg tggcatcagt agtaacaacc gtgtcacact
     4381 tgatccagta cgtctgagca acaaggaatt acgttggtta gcgagcaaaa aaaatcttgt
     4441 gattgcagta cacaagtccc aaacggctac cttgttgcat accgattcgc agcaacgggt
     4501 tcgtggtatt aatgctgatt atttaaatct tttaaaaaga gcgttaaata tcaaattaac
     4561 actccgggaa tacgccgatc atcaaaaagc aatggacgcg ctggaagaag gtgaagtcga
     4621 tatagtgtta tcacatttag ttgattcgcc acctcttaat gatgacattg ctgcaaccaa
     4681 accactgata attacctttc cggcgctcgt caccaccctt cacgattcaa tgcgaccgct
     4741 tacctcatca aaaccagtaa atattgctcg agtagcaaat tatccccctg acgaggtaat
     4801 tcatcaatca tttccaaaag caacaattat ctcttttaca aatttatatc aggcattagc
     4861 atccgtctca gccggacaga atgattactt tattggtagt aacatcatta ccagcagtat
     4921 gatttcccgc tatttcactc actccttaaa tgtagtgaaa tattataact caccgcgtca
     4981 atataatttt ttcttgacca gaaaagaatc tgtcgttctt aatgaagtac tcaatcgatt
     5041 tgttgatgct ttaacaaatg aagttcgcta tgaagtttca caaaattggc ttgatacagg
     5101 aaacctggcc tttctgaaca aaccattaga actcactgaa catgaaaaac agtggattaa
     5161 gcaacatccc gatttaaagg tgctggaaaa tccttactct cctccctatt ctatgacgga
     5221 tgaaaatggc tcggttcggg gcgttatggg ggatattctt aatattatta ccttgcaaac
     5281 aggtttaaat ttttctccga tcaccgtttc acacaatatc catgccggaa cacagcttaa
     5341 ccccggtgga tgggatataa tacctggcgc tatttatagt gaagatcgag aaaataatgt
     5401 ttcatttgca gaagcttcca taacaacgcc atacgttttt gtcatgcaaa aagcacctga
     5461 cagtgaacaa acattaaaaa aaggaatgaa agttgccatt ccatattatt atgagctgca
     5521 ttcgcaatta aaagagatgt atccggaggt tgagtggata aaagtcgata acgccagcgc
     5581 tgcatttcac aaggtcaagg aaggtgaact tgatgctctg gtcgcgacac agctaaattc
     5641 gcgttacatg atcgatcatt actatcctaa tgaactgtat cattttctta ttcccggcgt
     5701 tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa ccggaactta aggatattat
     5761 taataaagca ctgaatgcaa ttcccccaag cgaagttctg cgcctgacgg aaaaatggat
     5821 taaaatgccc aatgtgacca ttgacacatg ggacctctat agcgagcaat tttatattgt
     5881 tacgacatta tccgttttat tagttggcag tagcctttta tggggattct acctgttacg
     5941 ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa aaccaaatat cattccgaaa
     6001 agcactctcg gattccttac caaatccaac ttatgttgta aactggcaag gtaatgtcat
     6061 tagtcataat agtgcttttg aacattattt cactgcggat tactacaaaa atgcaatgtt
     6121 accattagaa aacagtgact caccctttaa agatgttttt tctaatgcgc atgaagtcac
     6181 agcagaaacg aaagaaaatc gaacaatata cacacaggta tttgaaattg ataatggcat
     6241 cgagaaaaga tgcattaatc actggcatac attatgcaat ctgccagcca gcgatcatgc
     6301 tgtttatatt tgtggttggc aagatattac cgagacgcgt gatttaattc atgcactcga
     6361 agtagagaga aataaagcga tcaatgcaac tgtcgcaaaa agtcagtttc tggcaacaat
     6421 gagtcacgaa ataagaacac caataagctc cattatgggc ttcctggaac ttctgtcggg
     6481 ttctggtctt agcaaggagc aacgggtgga ggcgatttca cttgcctacg ccaccggaca
     6541 atcactcctc ggcttaattg gtgaaatcct tgatgtcgac aaaattgaat cgggtaacta
     6601 tcaacttcaa ccacaatggg tcgatatccc tactttagtc cagaacactt gtcactcttt
     6661 cggtgcgatt gctgcaagca aatcgatcgc attaagttgc agcagtacat ttcctgatca
     6721 ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc ttatcaaatt tactgagcaa
     6781 tgctctcaaa tttaccaccg agggggcagt aaaaattacg acctccctgg ttcacattga
     6841 tgacaaccac gctgttatca aaatgacgat tatggattct ggaagtggat tatcgcagga
     6901 agaacaacaa caactcttta aacgctacag ccaaacaagt gcaggtcgcc agcaaacagg
     6961 atctggctta ggcttaatga tctgcaaaga attaattaaa aatatgcagg gcgatttgtc
     7021 attagaaagt catccaggca taggaacaac atttacgatc acaatcccgg tagaaattag
     7081 ccagcaagtg gcgactgtcg aggcaaaagc agaacacccc atcacactac ctgaaaagtt
     7141 gagcatatta atcgcggatg atcatccgac caacaggcta ttactcaaac gccagctaaa
     7201 tctattagga tatgatgttg atgaagccac tgatggtgtg caagcgctac acaaaatcag
     7261 tatgcaacat tatgatctgc ttattactga cgttaatatg ccgaatatgg atggttttga
     7321 gttgactcgc aaactccgtg agcaaaattc ttccttaccc atctgggggc ttacagccaa
     7381 cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc atgaacttat gtttgttcaa
     7441 accgttgacc ctggatgtac tgaaaacaca tttaagtcag ttacaccagg ttgcgcatat
     7501 tgcacctcag tatcgccacc ttgatatcga agccctgaaa aataatacgg cgaacgatct
     7561 acaactgatg caggagattc tcatcacttt ccagcatgaa acgcataaag atctacccgc
     7621 tgcgtttcaa gcactagaag ctggcgataa cagaactttt catcagtgta ttcatcgcat
     7681 ccacggtgcg gctaacatcc tgaatttgca aaagttgatt aatattagcc atcagttaga
     7741 aataacacct atttcagatg acagtaagcc tgaaattctt cagttgctga actctgtaaa
     7801 agaacacatt gcagagctgg accaggagat tgctgttttc tgtcagaaaa atgactaaat
     7861 agcggctccc acaatgttca aatgtgggag ctatttacca gcacatctta catttatgat
     7921 gagaactcct ggcgaatttg ttccccatgc tggtcgagcg ttgccgctcc cggcataaca
     7981 tgcgggtccg cgcagccgct gatttttatc ggattacccg gcatcattat tcccccggct
     8041 tcaatcaaca tatttctcac ctgagtttgt ggcaatttaa tggcctcagc cacacttaat
     8101 aacggcgcga cgggtacacc aacttcatgt attctggcta accaaacttc agctgcctgc
     8161 gtttttaacg tccgctcaat atattgttta agaatagcct ggttttgtac gcgtaaaata
     8221 ttgctgctaa atcggggatc attaaccagt tccgtaagct ccagtgcctg gcataacgca
     8281 g
//
LOCUS       DAJTNA010000165         8270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTNA010000165 DAJTNA010000000
VERSION     DAJTNA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.167"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..1204
                     /gene="dcuC"
                     /locus_tag="OO161_003264"
                     /pseudo
     CDS             1..1204
                     /gene="dcuC"
                     /locus_tag="OO161_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(1247..1744)
                     /gene="sspB"
                     /locus_tag="OO161_003265"
     CDS             complement(1247..1744)
                     /gene="sspB"
                     /locus_tag="OO161_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5464228.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1750..2388)
                     /gene="sspA"
                     /locus_tag="OO161_003266"
     CDS             complement(1750..2388)
                     /gene="sspA"
                     /locus_tag="OO161_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5464229.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2783..3175)
                     /gene="rpsI"
                     /locus_tag="OO161_003267"
     CDS             complement(2783..3175)
                     /gene="rpsI"
                     /locus_tag="OO161_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5464230.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3191..3619)
                     /gene="rplM"
                     /locus_tag="OO161_003268"
     CDS             complement(3191..3619)
                     /gene="rplM"
                     /locus_tag="OO161_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5464231.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(3838..4965)
                     /gene="zapE"
                     /locus_tag="OO161_003269"
     CDS             complement(3838..4965)
                     /gene="zapE"
                     /locus_tag="OO161_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5464232.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFMPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5159..5557
                     /locus_tag="OO161_003270"
     CDS             5159..5557
                     /locus_tag="OO161_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5464233.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5711..7078
                     /gene="degQ"
                     /locus_tag="OO161_003271"
     CDS             5711..7078
                     /gene="degQ"
                     /locus_tag="OO161_003271"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR5464234.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILASGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSAIAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7168..8235
                     /gene="degS"
                     /locus_tag="OO161_003272"
     CDS             7168..8235
                     /gene="degS"
                     /locus_tag="OO161_003272"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5464235.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT         1992 a         2093 c         2136 g         2049 t
ORIGIN      
        1 gctacttgat cgtcgatatt tataatgaaa tcctgcgcat gctgtccaac cgcattgccg
       61 gattggggct atcgattatg gcggtgggcg gtatgcccgc tacatggagc gcataggggc
      121 cagtcgcgcg atggtgagct tgttaagccg cccgttaaaa ctcattcgct caccgtatat
      181 tattctgtcg gcaacttacg tcatcggtca aatcatggcg cagtttatta ccagcgcctc
      241 cggtctgggt atgttgctga tggtcacctt atttccgaca ctggtgagtc tgggagtaag
      301 tcgtctctct gcggtggcgg ttatcgcaac cacaatgtcc attgagtggg gaattctgga
      361 aacgaactcc atttttgcag cccaggtcgc gggaatgaaa attgccactt acttcttcca
      421 ctaccagctt ccggtcgcct cttgcgtcat tatctcggtg gcgatctccc actttttcgt
      481 gcaacgcgct tttgacaaaa aagataaaaa tatcaatcac gaacaagcag agcaaaaagc
      541 tctcgataat gtcccgccgc tctattacgc cattttacct gtgatgccgt taatcctgat
      601 gctcggctcg ctgttcctcg cccacgtcgg gctgatgcag tcagaactgc atctggtggt
      661 ggtgatgtta ctgagtttga cggtgacgat gtttgttgag ttcttccgca agcataactt
      721 gcgcgaaaca atggacgatg tgcaggcgtt ttttgacggc atgggtacgc agtttgccaa
      781 cgtggtaacg ctggtggtcg cgggtgaaat atttgcgaaa ggcttaacga cgattggcac
      841 tgtcgatgcg gttatcaggg gtgcggagca ttctggtctg ggcggtattg gcgtgatgat
      901 tattatggcg ctggtcattg ccatttgtgc cattgtgatg ggctctggca atgcgccgtt
      961 tatgtcattt gccagtctta ttccgaatat cgcagccgga ctacatgtac cagcggttgt
     1021 aatgattatg ccgatgcatt ttgccacgac gctagcgcgc gcggtttcgc cgattactgc
     1081 ggtggtggtc gttacgtcag gaattgcagg cgtttcgcct tttgcggtgg tgaagcgaac
     1141 agcgatcccc atggcagtcg gtttcgtggt gaatatgatt gccacaatca cgctatttta
     1201 ttaagccatt aaaagacaaa acaggccacc agggcctgtt tttgtattac ttcacaacgc
     1261 gtaatgccgg tcgaccaccg cgtggtggct gcggaggttc atcgtcagga tgagtgtcat
     1321 catcgtgatc tggcttgtcg ccatcaataa ccgacataac ggtttcgttg tctgccgatg
     1381 cctcttcatc attcatgatg ctggtatctt catcgtaggc agcttcaggc tcaaacattg
     1441 tgcctgcgcc attttcacgg gcgtagatag ccagcacggc agccagcggc acagaaacct
     1501 gacgcggaat gccgccaaag cgcgcgttaa agcgcacctc atcattcgcc agttccagat
     1561 tgccgacagc acgcggcgca atgttgagta cgatttgccc gtcacgcgca tattccatag
     1621 gaacctgcac gccagggagc gtcacatcca ccaccaggtg cggcgtgagc tggttatcca
     1681 gcaaccactc atagaatgca cgcagcagat agggacgacg tggtgttagc tgtgacaaat
     1741 ccatacagat taactccggc ccagacgcat ttcacgttct gcttcagtta aagaagcaag
     1801 gaaagagtca cgctcaaaga cgcgggtcat atagcctttc agctctttcg cacccgggcc
     1861 gctgaactcg atgcccagtt gcggcagacg ccacagcagc ggagcaagat agcaatcgac
     1921 caggctgaac tcatcgctca ggaagtacgg cttctgaccg aagaccggcg caatcgccag
     1981 cagttcttcg cgcagttgct tacgtgcggc atctgcttca gaagctgaac cgttgatgat
     2041 ggtgttcatc agcgtgtacc agtctttttc gatgcgatgc atgtacagac ggctttcacc
     2101 gcgagctacc gggtaaacag gcatcagtgg cggatgcggg aaacgctcat ccagatattc
     2161 cataatgatg cgagattccc acagggtcag ctcacgatcc accagggtcg gaacgctctg
     2221 attcgggttg aggtcaatca gatcctgagg cggattgtcc ttttccacgt gttcgatctc
     2281 gaaacttaca cctttctcag ccagcacaat gcggacctga tggctataga tgtcagtagg
     2341 accggaaaac agcgtcatta ccgaacgttt gttggcagcg acagccatga aaacctccag
     2401 gtatagtcag aatttttact gctaccagcc accaggtggc cagtcagaag ttgtgttacc
     2461 cagtaaggaa cgactctctt tgttcgaaaa tcaaacaaaa aatgagcaat acccgacatt
     2521 tgggcagaaa attggatgat agtttaccag attttgcgac ctttgtggtg agtcgatgcc
     2581 ggaaatgggg aaaaagagat gcgctttagt ctgaaatagt tgacttagtc ccttattggc
     2641 gatgtggttt ttgttttacc tgtctgtcag gtggcagcaa aaagcaactt tccagttttt
     2701 acgctgattc agattttagc tataaaaaaa cccgccgaag cgggtttttt cgaaaattgt
     2761 tttctgccgg agcagaagcc aattaacgtt tggagaactg cggacgacga cgtgctttac
     2821 gcagaccgac tttcttacgt tcaacctgac gagcgtcacg agtaacgaag ccagctttac
     2881 gcagttcaga acgcagggac tcgtcgtatt ccatcagagc gcgggtgata ccgtgacgga
     2941 tcgcaccagc ctgaccagag ataccaccac ctttaacggt gatgtacagg tccagtttct
     3001 caaccatgtc gaccagttcc agcggctgac gaactaccat gcgggcagtt tcacgaccga
     3061 agtactgttc cagagaacgt tggttgatta cgattttacc gttgcccggt ttgatgaaaa
     3121 cgcgagctgc ggaacttttg cggcgaccag tgccgtagta ttgattttca gccattgcct
     3181 ataatcccga ttagatgtca agaacttgcg gttgctgtgc cgcgtggttg tgctcgttac
     3241 ccgcgtaaac tttcagttta cggaacatag cacgacccag cgggcctttt ggcaacatgc
     3301 ctttaaccgc gatttcaatc acacgctcag gacggcgagc aatcatctct tcaaaggtcg
     3361 cttgtttgat accaccgatg tggccggtgt ggtgatagta cactttgtca gtacacttgt
     3421 tgccggttac agcaactttg tcagcgttca gaacgatgat gtaatcaccg gtatctacgt
     3481 gcggagtgta ttccgctttg tgcttaccgc gcaggcgacg agccagttca gtagccagac
     3541 ggcccagagt tttaccggtc gcgtcaacaa cataccagtc gcgttttacg gtttctggtt
     3601 tagctgtaaa agttttcatt aaaagcttac ccaataaata gttacacgtt ggtgaacacc
     3661 caaacgtctt caattgttga ggttcacacg acaaagtccg gcaaacctac cccttcgaat
     3721 agcctatgcc agcacacaaa aagttttggg aaaaaaactt tcttgtaacg tggggtcgca
     3781 ggattataga gaagtcgggg tcaaagatcg accccttttt gtgatttgtg acaggtttta
     3841 acccgccaaa tgctcgcgct tcagatactc ttcgctttgc atctcttgca gacgtgacag
     3901 gcaacgctgg aactcaaact tcagccgatc gccctgataa atttcataca gcggcacttc
     3961 tgcactcacc actaatttga catggcgctc gtaaaactca tccaccagcg caataaagcg
     4021 ccgcgcttcg ctctccatca accgcgtcat aactggtaca tcaaacaaca tgaccgtatg
     4081 aaagaggcgt gagagcgcaa tatagtcatg ctggctgcgg gcatcgacac acaaggtggt
     4141 aaaagagaca gcaagcgtct gattctcagc gcccattgtc gccagcggtc gatggttgat
     4201 ttctaacgtc ggtgaatttt cgcgtttcgc tcccgccagc gccaaccata gtttatccat
     4261 ttgtgcccgg gtttcatcgt ggagtggcga aagccacaga tgcgcctgag tgagtgtacg
     4321 cagtcgataa tcaacaccag cgtccacgtt cattacatca caatgctgtt taatggcatc
     4381 gattgcaggc ataaaacgcg cacgttgcaa gccatttcga taaagttcgt ccggcggaat
     4441 atttgacgtc gctaccaggg taataccgcg ggcgaacagg gctttcatca gaccgccaag
     4501 tagcatggcg tcggtaatat cagaaacaaa aaattcgtca aaacagagca cgtcagtttc
     4561 ggctttaaag cgatcggcaa taatttccag cggatcggtc tgcccctgta aggcagttag
     4621 ctcttcgtgc acccgcagca taaaacggtg aaagtgcagg cgctgtttcc gctctcccgg
     4681 caggctttga tagaaaaggt ccatcagcca ggttttcccg cgtcctacac cgccccacat
     4741 atataagcca cgcactggcg tatgctttgt gtcttcgcgt ttaccccaca gcttaccgac
     4801 ccgcgccatt agcccactcg tcctgggggc tggtggcctg ctattgatga gttcttgata
     4861 aataatttcc aggcggctga cggcctcttt ttgaacgtcg tcgggttgat ggctgccttc
     4921 attgagcgcc ttcaggtatt gcgatgttgg ggtaacgctt tgcatgatct tattattatt
     4981 ccttgaataa tcggtgcgcc gttgttcacg gttgacgaaa aaaaggccgt tctacactac
     5041 gcgatatgca gtcgggattc cacttctgtg gaattaacgg ttatagtggc ataatcagcc
     5101 gcaggcatgg agcctgaagc caacacccta cggaaacaaa agacaacggg agatgttcat
     5161 gacctgggaa tatgcgctaa ttgggttagt cgtcggcatc attattggtg ctgtggccat
     5221 gcgttttggt aatcgtaaac tacgccagca acaggcgttg cagtacgaac tggaaaagaa
     5281 taaagctgaa ctggacgagt atcgcgaaga gctggttagc cactttgccc gcagcgcgga
     5341 attactggat accatggcgc acgactatcg ccagctgtat cagcacatgg cgaaaagctc
     5401 cagcagcctg ctgccggaac tgtctgctga agcaaacccg ttccgtaatc gtctggcaga
     5461 gtctgaagcc agcaacgatc aggcaccggt gcagatgcct cgcgactatt ctgaaggtgc
     5521 atccggcctg ctgcgtactg gcgcgaagcg cgactaattt atttttcggg cgcagccatt
     5581 gcgccctcct cttctctccc tccccgacta tcatttaatc tggtgtctca ttgttagccg
     5641 tctgaaaatt caataacatc aaactgtttt gaatctcttt tcttatcatt caggtacgag
     5701 agcaggaata atgaaaaaac aaacccagct gttgagtgca ttagcgttaa gtgtcgggtt
     5761 aactctctcg gcgtcatttc aggccgtcgc gtcgattcca ggccaggttg ccgatcaggc
     5821 ccctctcccc agtctggctc caatgctgga aaaagtgctt ccggcagtgg tgagcgtacg
     5881 ggtggaagga acggccagtc agggacagaa aatcccggaa gaattcaaaa agttttttgg
     5941 tgatgattta ccggatcaac ctgcacaacc cttcgaaggt ttaggttccg gtgtcatcat
     6001 caacgccagt aaaggctatg tgctgaccaa caaccatgtg attaatcagg cacagaaaat
     6061 cagtattcag ctcaatgatg ggcgcgagtt tgatgcaaaa ctgattggta gcgatgacca
     6121 gagcgatatc gccctgttac aaattcaaaa cccgagcaaa ttaacgcaaa tcgctattgc
     6181 cgactccgat aaattgcgcg tcggtgattt tgccgtagcg gtcggtaacc catttggcct
     6241 tgggcaaacc gccacctctg gcattgtttc cgcattaggc cgcagcgggt tgaatcttga
     6301 aggtctggaa aactttatcc agacagatgc ttccattaac cgcggtaact ccggcggtgc
     6361 actattaaac cttaacggtg agttaattgg catcaacact gcaatccttg cgtctggcgg
     6421 cgggagcgtc gggattggat ttgccatccc cagtaatatg gcgcgaacac tggcgcagca
     6481 gcttatcgac tttggtgaaa tcaaacgcgg tttgttagga atcaaaggca ccgagatgag
     6541 tgccgatatc gccaaagcct tcaaccttga cgtgcagcgt ggcgcgtttg tcagcgaagt
     6601 gttgccaggt tctggctcgg caaaagcggg cgtcaaagcg ggcgatatta ttaccagcct
     6661 caacggcaaa ccgctgaata gctttgctga gttgcgctct cgtatcgcga ctacagagcc
     6721 gggcacgaaa gtgaagcttg gcctgctgcg taacggcaaa ccactggaag tagaagtgac
     6781 gcttgatacc agcacctctt cgtcggccat cgctgaaatg atcacgccag cgctggaagg
     6841 tgcaacgttg agcgatggtc agctaaaaga tggcggcaaa ggtattaaga tcgatgaagt
     6901 tgtcaaagga agcccagctg ctcaggcagg cttgcaaaaa gacgatgtga tcattggcgt
     6961 caaccgcgat cgggtgaact cgattgctga aatgcgtaaa gtgctggcgg caaaaccggc
     7021 catcatcgcc ctgcaaattg tacgcggcaa tgaaagcatc tatctgctga tgcgttaatg
     7081 tcgtaaaccg ggcatcaggc ttacgtgtga tgtccggtta actcgtggta tgctgctgcc
     7141 gttccctttt ttaatgacgc ctccatcatg tttgtgaagc tcttacgttc cgttgcgatt
     7201 ggattaattg tcggcgctat tctgctggtt gccatgcctt cgctgcgcag ccttaacccg
     7261 ctttccactc cgcaatttga cagtaccgat gagacgcctg ccagctataa tctggcggtt
     7321 cgccgcgccg cgccagcggt ggttaacgtt tacaaccgtg gtgtgaacac caactctcac
     7381 aaccagcttg agatccgcac cctgggatcc ggtgtaatca tggatcaacg cggttatatc
     7441 atcaccaata aacacgtcat caacgacgcc gatcagatca tcgtcgcctt acaggatgga
     7501 cgtgtatttg aagcattgct ggtgggatct gactctctaa ccgatctggc ggtacttaaa
     7561 attaatgcca ctggcggttt acctaccatt ccaattaatg cacgtcgcgt accgcacatt
     7621 ggcgacgtag tactggcgat cggtaacccg tacaacctcg ggcagaccat tacccaaggg
     7681 attattagtg ccacgggtcg aatcggtctg aaccctaccg ggcggcaaaa cttcctacaa
     7741 accgatgctt ccattaacca cggtaactct ggcggcgcgc tggtgaactc gctgggagaa
     7801 ctgatgggca ttaacacgct gtcgtttgat aagagtaacg atggcgaaac gccggaaggt
     7861 atcggctttg cgattccttt ccagttagca accaaaatta tggataagct gatccgcgat
     7921 gggcgcgtga tccgcggcta cattggcatt ggtggacgtg agatcgcacc actgcacgcg
     7981 cagagcggtg gtatagatca actgcaaggg atcgtggtta atgaagtgtc acctgacggc
     8041 ccggcggcga atgcgggtat tcaggtcaac gatctgatta tttcagtgga taacaaaccg
     8101 gccatctctg ctctggagac gatggatcag gtggcagaaa ttcgccctgg ttcggtgatc
     8161 ccggttgtag tgatgcgtga tgataagcag ttaacgctgc aggtcaccat tcaggaatat
     8221 ccggcaacca attaagtcga gtgctcaaaa caaaaaaccg gagtctgtgc
//
LOCUS       DAJTNA010000166         8235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTNA010000166 DAJTNA010000000
VERSION     DAJTNA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.168"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(8..400)
                     /locus_tag="OO161_003273"
     CDS             complement(8..400)
                     /locus_tag="OO161_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5464236.1"
                     /translation="MRDIQMVLERWGAWAANNHENVSWSPIAAGFKGLIPSKVKYRPQ
                     CCDDDGLIISSVMAVLKKKEPYQYQLLEMYYVYGITLRALGAKMGISLNQVVIRLQKA
                     EGFVEGCLAMLEVSLEMDCYMNHENDSR"
     gene            complement(418..1407)
                     /locus_tag="OO161_003274"
     CDS             complement(418..1407)
                     /locus_tag="OO161_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5464237.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDGLHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(1459..1716)
                     /locus_tag="OO161_003275"
     CDS             complement(1459..1716)
                     /locus_tag="OO161_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464238.1"
                     /translation="MNNRTITVSPEQLRRQAQEMLRCAEQMEKTSVKKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(1713..3113)
                     /locus_tag="OO161_003276"
     CDS             complement(1713..3113)
                     /locus_tag="OO161_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5464239.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFF
                     VRQYRDIYAGICRQARISGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAYTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVGRVECRNQGLEKSRTLMTGINELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSTAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRYHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPDTQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            complement(3110..3988)
                     /locus_tag="OO161_003277"
     CDS             complement(3110..3988)
                     /locus_tag="OO161_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5464240.1"
                     /translation="MMTIDQREKQTRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGKYRTHILVWIDRNGEDREKISCCPDCLIAEASDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIVDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYLSRIGGVAA"
     gene            complement(3999..4823)
                     /locus_tag="OO161_003278"
     CDS             complement(3999..4823)
                     /locus_tag="OO161_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5464241.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNYITALCESGLVKKVSRKGEKGNSSNIYLLRLDGAKDSPGGSANNSL
                     PSAANSPGGVGVAPGGSAGDSPRTSHSFEPVKEPVNESTIGASADASAPARSARQEYS
                     PEFEQAWQEYPKRAGGNSKSAAFKAWKARIREGIKPETMLDGVKRYAAWVRATGNTGT
                     QFVKQAATFFGPDRHFEDYWQQPAAHGGGRQRQVDVLAGLGAMSDKFGKSSNKLTF"
     gene            complement(4820..5044)
                     /locus_tag="OO161_003279"
     CDS             complement(4820..5044)
                     /locus_tag="OO161_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464242.1"
                     /translation="MIRHIVNSLYHRYNRCPRVGQWFATSNGHVLRVCLVNAESQKVV
                     CELQGRSYTISYPLAVFLSGKMFKRLGGVL"
     gene            complement(5041..5235)
                     /locus_tag="OO161_003280"
     CDS             complement(5041..5235)
                     /locus_tag="OO161_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464243.1"
                     /translation="MISYEIIISTTEYRNDVSVRTDVSVWHRRYKSRKTAELKAAEMC
                     ETISMKGSPVKYVTTAEVRP"
     gene            complement(5232..6083)
                     /locus_tag="OO161_003281"
     CDS             complement(5232..6083)
                     /locus_tag="OO161_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5464244.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKVGAGGRSPELSTAIYDAPASF
                     LSSALTHARIMVGWAGEPKGSPVSVDAGTANPVQSATSEFRSSGGGSFPQSTEAAIMA
                     TIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTLECSASFTARNFSVSDY
                     TDCTGRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMEKNLSGADAVDMSA
                     VARNARGVYLHLREIHQIWTSQLYPMLKAIESPLASKLYDRVGDAVFGAALVDSRLNG
                     SDKEVRP"
     gene            complement(6192..6899)
                     /locus_tag="OO161_003282"
     CDS             complement(6192..6899)
                     /locus_tag="OO161_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5464245.1"
                     /translation="MNTAIFNGKASMTSVEIAELVGKRHDNVKRTIETLAKGGVVRSP
                     QIEVSERINNLGFKVQYEHYLFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVSHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            7235..7891
                     /locus_tag="OO161_003283"
     CDS             7235..7891
                     /locus_tag="OO161_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5464246.1"
                     /translation="MKSLGERLINARQKAGLTQDALAKKAGITRVAISKAEQGLTKSF
                     NGDTLFKVAAALRCSPQWLQNGDEKDKHWENNVKSCPQRDTAHSYPVINWVQAGLFAT
                     SGDDYNMYDQDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKEVFS
                     GCYVIARKKSTNEATFKKYISDMGKAFLKPLNPQYPIIEMDNDCEIVGVVVDARWDIF
                     "
BASE COUNT         2056 a         2211 c         1886 g         2082 t
ORIGIN      
        1 tgttcattta tcttgaatca ttttcgtgat tcatatagca atccatttct aaagatacct
       61 cgagcattgc cagacaccct tcaacaaacc cttcagcttt ctgcaatcta ataacaactt
      121 gattaagcga tatccccatt ttcgccccta acgctcgtaa tgtgatccca tatacataat
      181 acatttccag taattggtat tgatatggtt cctttttctt gagaactgcc ataactgaac
      241 taatgataag accatcatca tcgcaacatt gcggtcgata ttttactttt gaggggatga
      301 ggcctttaaa ccccgcagca attggcgacc aactaacatt ctcatgatta tttgccgccc
      361 atgctcccca gcgttcgaga accatctgaa tatcacgcat caactttctc cacaaaatca
      421 ggacagcaca cctatcgcca gcgcgcgatc gataaaacga aatatcagct ccagttggga
      481 accatacttc tcttcaaatg ccacggtatc cgcatgcagt ccgtcatggt gttttctgca
      541 caaaggcaac acaaaaaggt catgcgcttt tgtacccatt ccaccctgac catgaccaat
      601 caggtgatga ggatcgtcgg ctggcttacc acaacatgca cacggctgcg tcttaaccca
      661 gcgcgtgtac ttttcattaa cccagcgacg acgtttgggg cgtaacataa aagactccgg
      721 cgactccgga tccactttca gcgccagcac ctttttcgcc ttatcccgga tgatgctggt
      781 ggcaggaacc gaaggcacaa ggtcactttc ccgggtgaca gacggcacaa caggcttcgg
      841 taatctcagt gccttacggg ctgcactttc cggtaaggca tccgccaggt cattacgaac
      901 cagccaccag cacagttccg gcattgtcac aacgtggctg tcatcaaaac cgagatcacg
      961 acggactacg gacaacaccc agcgggcaca gttatccgtt gccattgatt ccagacgttc
     1021 cgtgaactga tcgcgaagca ggttatcgca gtgccagcac agacggattg cgcccggcgc
     1081 gtgtcgcatt gtggtcatgt tctcgctgtg ccatccggaa tgaggccact ggcagccttt
     1141 ttcacgaagt aaccagctct caaggcattc cacgccacca gcacgacgga tcaccgcctc
     1201 atggcggaac acggcccgaa cggcaggatc atccgccagc ggttgtgatg ctgccggaac
     1261 ggcaccactg gcaaaagatg aataacgttc cggctcaggc tccagcagga cacgcccctg
     1321 cataaacagg ggcatcagct ctgaaccggg tctgaacaag acgatcccca tacgcggggc
     1381 aatttcaggg gtcagcagtg ctctcacggt cacctcagca aacgatattg aatgcataca
     1441 gagaaaaaaa ctcagtcatc acgcagtaaa ctccttcacc agcgtttcaa actggcttac
     1501 ctgtccttcc agttccgcca cgcaatccac cagctcatcc accgcctttt gtgtgcggtg
     1561 ttttgcctgc agcagatcac gaagcgccgg agtaagctgc ttgcggagcg tatctttttt
     1621 cacgctcgtt ttttccatct gttcagcaca acgaagcatc tcctgcgcct gccgacgaag
     1681 ttgttccggt gaaacagtga ttgttctgtt gttcaaaata aacgctccgt tttactgccc
     1741 gacatgcggt tattgctgta tctgcgcgga ttgcccggcg tcatgggtgt ggaaagaacc
     1801 cgggcactct cctggtccac aggcagaaaa tgtccgttat gaaaacgccg gtaaatggtc
     1861 ccgagtgtgc cattacgctg tttcgtgatg ttgatttctg ctatgcctct ggcctgtgta
     1921 tccgggttat acacctcatc cctgtaaagc atcagaatga tgtcggcatc agcctctatt
     1981 tccccggagt ttttcaggtc tgagttcatg ggacgtttat tgggtctgga ttccacgcct
     2041 cgggagagct ggctcagagc aatcagcggg aaaccgccgg attttgccag gcttttaagt
     2101 ccctttgaga tttcccccac agcaaggtcg tgacgccccg tgctgcgggt tttaatcaga
     2161 ccgaggtaat cgaccaccac cagcgccgtt tccggatgct tcatccggtg gtacctcgtg
     2221 gttgcacata tctcatcaat tgttagattt gcctggtcca ccatccagat attacgcccc
     2281 gtcattcgtc ccacgccctg cgagaaacga gcccagtctt catcttcaaa acgggcaaca
     2341 gacttaagac gggataccgg cattccaccg gcagtagaca ccatacgttc accaatctgg
     2401 atgttcgcca tctccatggt gaacagaagc acgccatgcc cctgctcagt caccttgtcg
     2461 atgatgtcca gcgccagttc ggttttcccc atcgaaggac gagccgcaat gaataccagg
     2521 tcgcctggct ccatcccccc tgtttttgcg tccagttcat taataccagt catcagtgtt
     2581 ctggatttct ccagtccctg attccggcat tcaacacggc ccaccacttc cggaaggaca
     2641 tcatcaatgt gaaccggctg aatgacgccc tttccggtcg acagtgaggc catcatgttc
     2701 tgcgcatcct tcagggcatc ctcggctgct tcacaggtat acgcatcacg taaattctgt
     2761 aatgcttcag tcagtgtttt ttctgcatca cgcagtgcgg cattgcgccg caacgctgcg
     2821 acatagtgct ccagtgaaga cttcacccag gttttgcgtc cggtgtcggt aatcaccggg
     2881 gcaagttccg gcatctcatt gcacagcagt acggggtcaa tgacgccgga tatgcgagcc
     2941 tgtctgcaaa tccccgcgta aatatcccgg tactgacgca caaaaaatac atccgccgga
     3001 agtgtggcca gaatatccat cacttccgga tcggccccac gcagaaaaaa cgcaccgatg
     3061 acagcgcctt ccaggtcatc gttacgccat gccggggtgt tctggctggt catgcggcaa
     3121 cacctccgat acgagaaagg tagctgggcc agttaaacga caaccagttg cgcccgccat
     3181 cggtgatcct gtcggcaatc cggggactga tgaacgccca caattcttcc ggtgaaaggt
     3241 tgctgatcag gatagttggc aaaataccct cataccgggc attgataatt tcctgcaaaa
     3301 tggccatttc agccgcactg ccaaactgaa cgccgacttc gtcgacaatc agcaaatcca
     3361 gtgacgcata atgctcaatg acgtcatccg ctgttttttc actgtcattc cgccagcagt
     3421 ttttcacagc ccgggtaagg cgcatcacgt cggtgatctc cacactggcc agatagttac
     3481 ggatgatgtg ttttgccatt gataccgcca gatgattttt cccggtaccg caactgccgg
     3541 tcataacaag actggtaccg ttctccagca tatctggcca gttctccgca tagcggcgac
     3601 aggccgcaag atttctggct gcgtcaggat taacctccag ataattatca aactcgcagt
     3661 cccgaaaacg cagagcaatt ccggcgttat cagtcagttc ttccgcctta agggacgaca
     3721 gttccatggt caaatcactg gcctcagcga tcaagcagtc agggcagcat gaaatttttt
     3781 ctctgtcctc gccattacga tcgatccaca ccagtatatg cgtacgatat ttaccgtgtt
     3841 tttcgcaata tccgcgacct tcacgcatca ggcaggaacg ataaggccat ggcttttcgc
     3901 ccttctgagc aaatgcaatc tctgcccgta actcatccat tcgcgcctgt agtcttgttt
     3961 gtttctcacg ttggtcaatc gtcatcatcg ctgtcacctc agaatgtcaa tttgttactg
     4021 gatttaccga atttgtcaga catggctccc aggccagcca ggacatcgac ctgtcgctgt
     4081 cgcccacctc cgtgagcggc tggctgttgc cagtaatctt cgaagtgacg atcgggtcca
     4141 aagaacgtcg cagcctgctt cacgaactgt gtgccggtat ttcctgtagc acgtacccag
     4201 gcggcatacc gcttcacgcc atcaagcatg gtctccggtt ttattccctc cctgatacgg
     4261 gctttccagg ctttgaaggc tgctgacttg gaattgccac cagcacgttt gggatattcc
     4321 tgccaggcct gttcaaattc cggtgaatat tcctgtctgg cagaacgcgc tggtgcagac
     4381 gcgtcagcgg atgcgccaat agttgattca ttgactggtt ctttgactgg ttcaaaagag
     4441 tgactggttc tgggtgaatc tcctgcacta ccccctggtg caactcctac accacctggt
     4501 gaatttgctg cactaggtag tgaattattt gcactaccac ctggtgaatc ttttgcacca
     4561 tcaagacgaa gaagataaat attgctcgag ttcccttttt cacctttccg ggaaactttt
     4621 tttaccagtc cagattcaca aagagccgta atataattca tcacagaacg tttgctaatc
     4681 tcgcactgat cagcaatatg ctgatagctg ggccagcact cgccctgatc gctggcatta
     4741 tcagccagct taatcagaac cagttttcgc aacggatttc ccacacgaat tttcatcgct
     4801 ttcaccatca gctccatgct cataacacac ctcccaggcg tttaaacatt tttccagaca
     4861 gaaataccgc cagagggtaa ctgatggtgt agctacgccc ctgtagttcg cacacgactt
     4921 tctggctttc agcgttgact aggcaaaccc gcagaacgtg accgttgctg gtggcgaacc
     4981 actgccccac acgggggcaa cggttgtatc ggtgatacag ggaattaacg atgtggcgga
     5041 tcatggacgc acctccgccg tagttacgta tttaaccggg ctacctttca ttgagatggt
     5101 ttcacacatc tctgccgctt tcagttccgc tgtttttctg gatttatagc gacggtgcca
     5161 gacagataca tccgtgcgaa ctgatacatc gtttctgtat tccgtagtgg agatgatgat
     5221 ttcgtaacta atcatgggcg aacctccttg tcagaaccat tcagcctgga atcaacaagt
     5281 gcagcgccaa aaacagcatc accaacacgg tcgtacagtt tgctagccag cggagattca
     5341 atggccttaa gcattggata aagctggctt gtccagattt gatggatttc acgcaaatgc
     5401 aggtatacgc ctctggcgtt tcgtgcgaca gctgacatat caaccgcgtc agcaccagat
     5461 aaattcttct ccatctggtt aaaggcgttg atgtatgcct ctttgaaccg ggctgcacgt
     5521 ttgccagtaa agcccatagc aaggaacgcg aagccatcac gggtgatttg atagcaaggt
     5581 agtttgcggc ctgtgcaatc ggtgtaatca ctcaccgaaa aattgcgggc agtgaatgat
     5641 gcggagcatt caagcgtgcg gatctttttc agtacatcgt catgacgctt ggagaagaag
     5701 ttggcaacag ccagggatga agtaacagcc tgaccatcaa cgatggcaat ttcaggttga
     5761 gtgagggttg ggatcgtagc catgatggca gcctccgttg actgtggaaa acttccacca
     5821 ccggagctgc gaaactcact ggtggcagac tgaacagggt tcgcagtacc ggcgtcaacg
     5881 gagaccggcg agcctttcgg ctcccctgcc cagcccacca taattctggc gtgcgtgagc
     5941 gcggacgata aaaaagacgc tggcgcgtca tatatcgccg ttgacaattc cgggctgcga
     6001 cccccggcac ccactttata aggtgcagag acagtgtaac gtcccgaaat tgcagaatca
     6061 atatttggtc ttgaaatgat catatagctg ctgatatctt tagaactgtt cttggatgtt
     6121 tcggagccgt tttatgcgaa acagctcccc gttattgatg ttgagtgagc cgggttactc
     6181 ccggcttttt ttcaccgctg ccaaccaata acctgaaata accccatttt cgggtgatac
     6241 cagcgagtcc ctcgcggttc tgcttcctcc ataacccgat aaaaagcagc cataaacggt
     6301 tccacagcaa caattgcgcg acgtgacaac aatccgtccg gcgtcatgaa ctcatgggtg
     6361 tctgtaggaa tctgataggc gttcaccaga ttgcggcatt tatcatctga caaaccggtt
     6421 tttgctttca gttggcgata tccggcatag ccctcacgaa tagtgccctt tttaatttgc
     6481 tcgactgttt cagcaacgtg gctgactttt tcttccacct gagtgatccg tttctgctga
     6541 cgaactgctt caagagccat cgcggcaacc atttcgattt cgctcattgg cttacggatc
     6601 tgttcttcca gttcgcgcca gcgatctacc aggcgagcag tgaattcagg acagagctgt
     6661 gcgacgacaa tgatgctgtc gcgcttacct tgttctcctt caaacaggta atgctcatat
     6721 tgaactttaa aacctaagtt attgattctt tcggaaacct caatttgagg agaccggaca
     6781 acgccacctt tggctaatgt ttcaatagtg cgtttcacat tgtcatgacg tttacccacc
     6841 agctctgcga tctcaacgct ggtcatggat gctttgccgt taaaaattgc ggtgttcatt
     6901 gttggtctcc tgtgggcttg tcatcttctg tattcgctag acttgggtgt gtatatggaa
     6961 tgctcggatc cagatgacaa agaatggcaa catcctccgg aacacctcgc gttttccact
     7021 ttccaacacc ttgactgcca cgaggccttc ctttctttgg gaacctgcga ccaatagcgg
     7081 cattggtttt aaattgaatt tttaatattt cataaagggt cattctttag tctcacacca
     7141 gatactttgt tatccaacga tgttaaccgc aagaatccaa agtatcaaga tattctgtta
     7201 ctttagtatc aacagccatg agaggagaag aaaaatgaag tctttaggtg aacgtctcat
     7261 caacgcacgg caaaaagctg ggttaacaca agatgcgttg gctaaaaaag ctgggatcac
     7321 cagagttgca atcagtaaag ccgagcaagg ccttacaaaa agtttcaacg gtgacaccct
     7381 ttttaaagtt gcagctgcac tgcggtgttc accgcagtgg cttcagaacg gagatgaaaa
     7441 agataagcat tgggaaaata atgttaagag ctgcccacag agagacacag cacactctta
     7501 ccctgtaatt aactgggttc aggcaggatt attcgcaact tctggtgatg actacaacat
     7561 gtatgatcag gataattgga ggcattctgt aaaatacgct ggtgagaggg ggttctggct
     7621 ggaagtgcac ggagactcaa tgacttcgcc cgtaggaata acatttcctg aaggaatgtc
     7681 gatccttgtc aacccagata aagaagtttt ttcagggtgt tacgtcatcg ccagaaaaaa
     7741 atccaccaat gaagcaacat tcaaaaaata tatttctgac atgggaaagg cgtttctaaa
     7801 gccccttaat ccacaatatc caatcataga aatggacaat gattgcgaaa tagtaggtgt
     7861 tgtggttgat gccaggtggg atattttctg accagacaca aagcacaaaa agaaaacaaa
     7921 gtatcaaaaa tcacttgtca caccttgata ccttagttac cataaaacaa agttcgtaac
     7981 tgaggtatca tctcatgttc aataaagcta caactcttga ctgtctcgaa gaactgaaaa
     8041 acctcggcag cctcattaca ctaatagcaa aagcaacacc tgatgctacg ctctctagcg
     8101 atatagagtc atgcgcagga ctggcatggg atatgacaaa tagcatatcc agaaagctat
     8161 cgtcagcaat gcttttacag aacaaaaatt ctgcaatcaa caaccgtctt cgcacccaac
     8221 gcgaagcctg cggct
//
LOCUS       DAJTNA010000167         8228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTNA010000167 DAJTNA010000000
VERSION     DAJTNA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.169"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            103..831
                     /gene="mpaA"
                     /locus_tag="OO161_003284"
     CDS             103..831
                     /gene="mpaA"
                     /locus_tag="OO161_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5464247.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(806..1771)
                     /gene="ycjG"
                     /locus_tag="OO161_003285"
     CDS             complement(806..1771)
                     /gene="ycjG"
                     /locus_tag="OO161_003285"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5464248.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1890..2396
                     /gene="tpx"
                     /locus_tag="OO161_003286"
     CDS             1890..2396
                     /gene="tpx"
                     /locus_tag="OO161_003286"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5464249.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNSEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2440..3981)
                     /gene="tyrR"
                     /locus_tag="OO161_003287"
     CDS             complement(2440..3981)
                     /gene="tyrR"
                     /locus_tag="OO161_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5464250.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDAIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVSRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4129..5190)
                     /locus_tag="OO161_003288"
     CDS             complement(4129..5190)
                     /locus_tag="OO161_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5464251.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5187..6584)
                     /locus_tag="OO161_003289"
     CDS             complement(5187..6584)
                     /locus_tag="OO161_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5464252.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGVPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            6739..7737
                     /locus_tag="OO161_003290"
     CDS             6739..7737
                     /locus_tag="OO161_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5464253.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(7848..>8228)
                     /gene="ompG"
                     /locus_tag="OO161_003291"
     CDS             complement(7848..>8228)
                     /gene="ompG"
                     /locus_tag="OO161_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR5464254.1"
                     /translation="TGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYLP
                     LTLGNHSVTPYTRIGLDRWSNWDWQDDIECEGHDFNRVGLFYGYDFQNGLSVSLEYAF
                     EWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1997 a         2273 c         2003 g         1955 t
ORIGIN      
        1 aaagtgatac aagatcgatg acatagcgtt gaagaaaagg atttgataat ccgcaaaatt
       61 tgcacaacac tgcctgtaat acttcctgaa ccagagacga tcatgaccgt aacccgccca
      121 cgcgccgaac gcggcgcatt tccgcccgga acagaacatt acggacgttc attattgggc
      181 gcgccgttaa tctggtttcc tgcccctgca gccagccgcg aaagtggttt gattctggct
      241 ggcactcacg gtgatgaaaa ctcttcggtc gtcacgctct cctgtgctct gcggacgttg
      301 acaccttctt tgcgccgcca tcatgtggtg ctgtgtgtga atcctgacgg ctgccagctg
      361 gggttacggg ccaatgctaa tggtgtggat ttaaacagaa actttccggc ggcgaactgg
      421 aaggaaggtg aaacggttta tcgctggaac agcgccgctg aagaacgtga tgtcgtatta
      481 ctgacaggtg acaaacctgg ctcagaacct gaaacccagg cgctgtgcca gcttatacac
      541 cgcattcaac ctgcctgggt ggtctcattc cacgatccac tggcctgtat tgaagatccc
      601 agacacagcg aattaggcga atggctggcc caggcgtttg aattgcctct ggtaaccagc
      661 gtcggttatg aaacgcctgg ctctttcggc agttggtgtg cagatctcaa cttgcattgc
      721 atcaccgcag agtttccgcc catctcctcc gacgaagcca gcgaaaaata tctctttgcg
      781 atggcaaact tgctgcgctg gcatcctaaa gatgcaattc gcccgtcgtg aactgaagcg
      841 ccggttccac atctaccgcc agccaggtcg gtccgtcaag atctgcgaaa ctgacctgcg
      901 gcaccagcgg taaagcggcg ctaatggcac gagaggtaca caacatgcag cccagcatca
      961 gactgaaacc ttgtgcacgc gcttcagtcg ccagcgccag cgcttccgtc agaccaccgg
     1021 ttttatcgag cttaatgtta accatctcat agcgcccttt cagcgccttc agattgctac
     1081 gtgtatgaca actctcatca gcacaaatcg gcaacggatg aataaaattc tccagcgccg
     1141 catcgtcctg cgcaggaagg ggttgttcaa gcatcgcaac gcctaaatcc gccaatagct
     1201 ggcaacgcgc cgccaaccct tctgcacgcc aggattcatt tgcatcaacg atcagcgtcg
     1261 catcgggcac agctgtgcga attgccacca tccgctcact aataagatgg ttatccagct
     1321 tcactttcag taatttcgcg cctgcctgcc agagtgttga tgcactattg gccatctgat
     1381 caggcgtacc gatgactacc gtctgtgcag tgataactgt ctcgggaagc gttatgccga
     1441 tcaaatcagc cagcgattgc tgctgttttc gcgcggccag atcccacaat gcacaatcca
     1501 gcgcattacg tgctgcgccg gcagggagaa ttttttgcaa ctcctcccgt gtcagccctt
     1561 tctctaattg cgacacgacg ctcataattt gcgccattac cgaggcatca ctttctccat
     1621 aacgcggata cggcgtgcat tcgccggtgc ctttaatacc ctcttcttcc agttcaacca
     1681 ccaccacgcg cgcttcactg cgacttcccc gggcaatcac aaacggggta tgtaagggcc
     1741 aggcttcctc aaatacctta acggttctca tctttactcc ttgttgtcgc ggtggtctgc
     1801 ggattaagcg caaatagcgt ttgctgtgtt attgacagtt agcataaact aggtgtgacg
     1861 ttaactatat gtaaacagga aagataatca tgtcacaaac cgttcatttc cagggcaacc
     1921 cggttacagt cgccaattcc atcccgcagg cgggtagcaa agcgcagact tttactctcg
     1981 tggcaaaaga tctgtctgac gtcaccctcg gtcagtttgc gggtaaacgc aaagtgctga
     2041 acattttccc gagtattgat accggtgttt gcgccgcatc agtacgtaag tttaaccaac
     2101 tggcaaccga gatcgacaac accgttgtgc tgtgtatttc tgccgatctg ccgttcgccc
     2161 agtctcgttt ctgcggcgca gaaggtctga acaacgttat caccctctcc actttccgta
     2221 actctgaatt tctgcaagcc ttcggtgtgg caattgctga tggcccactg aaaggtctgg
     2281 cagcgcgtgc cgttgtggtt attgacgaaa atgacaatgt gattttcagc cagctggtgg
     2341 atgaaatcac caccgagccg gattacgaag cagctctggc tgtactgaaa gcataagtta
     2401 atatgcctga tggtgttgca ccatcaggca tattcgcgct tactcttcgt tcttcttctg
     2461 gcttaagcca tattcccgca acttattggc aatcgcggta tgtgaaacgc cgagacgttt
     2521 tgccagtttg cgcgtgctgg gataattgcg ataaagctgg gttaataccg agcgttcaaa
     2581 acggctggtg atttcgtcca gcgaaccttc catcgcatct tcgcccacgg ctaccgttgc
     2641 ggcgtcataa tccggcaaca aaatatcctg tggacgcagc tcataaccgt ccagttgtgt
     2701 cagtgcgcga tagatagcgt tctttaactg ccgcacattt cccggccacg cataacgcgt
     2761 aagtacagta ttcaggtcag cggccagttt cggacgcgac acgccctgct cgtcggcaaa
     2821 gcgggcgacg aacagctcag ttaacggcat gatgtcctgc ggacagtcac gtagcggcgg
     2881 cagattgagc gtcaacacgt tcagacgata atagagatct tcacggaaca tgcctttttg
     2941 caccagttcg accagattct tctgcgtagc gcaaatcacc cgcacatcga catgcacctc
     3001 atggtcttcg ccaacccgac ggaaagtgcc atcattaaga aaacgcagta atttcgcctg
     3061 catccgtggt gacatttccc ctattgcatc caacagcacc gaaccaccgt tcgcctgctc
     3121 aaagaatcct ttcttccctt ccggagcatg accaaacagt tcactctcga ccgcatcttc
     3181 cggtatagac gcacagttca gcgccaggta aggtttgccc gctctggggc ttgcctgatg
     3241 gcaggcgtag gcaaagagat ctttacctgt acctgtgtca cccgtaatca gcagcggcgc
     3301 gcttagcatc gccagtttct gcgcctgttc gacaacatgc ttcattttcg ggctgacggc
     3361 gacaatttga ctgaaggcgc tgacgtcctg ggcggcgaca ttttgcaact ggcggcccat
     3421 acgaatcgtt gatcgcaaca tcaccaccgc accggtcagg acgtgttgat cattttcatc
     3481 ctgaagataa acaggcgtaa tctccatcag gaaattctgc ccattaataa cgacatgctc
     3541 gttatgcgaa tcttgcggtt cgctttccag ccaacgtaaa aaattaaagc cgttaatcaa
     3601 ttgtgcggcg gtatggttgc gcaggcgatc caatttttgc ccaaaaagct gacagctcgc
     3661 cgggttcgcc atatccactt tgcttttcat atcgacagag agcacaggtt cgggcaacgc
     3721 ctccagtaac gcgctcaacg ccagatgctc acgttcggaa ggcatccacg ggacagtacg
     3781 cacatcggta acacctgcaa tacggcgtat ttcggccatc agactgctga aactctcaaa
     3841 ctccagttca gcaaaattga ggtagattcg cccaatggga tcaatctcaa taccgcgtaa
     3901 atcaatgcct cttagcacga gtagatcgag taattcgcgg gtcagaccga gtcggtcttc
     3961 acaaaagact tccagacgca tgggaacctt cacctgaaaa aagaacaatt aatatgatga
     4021 tatgacacta tttggatagc aggaaggttt ctgtcaacaa tcattgacac aaagacggaa
     4081 aaacgggcac ttcggcgtaa agatatccgg tcagcacccg atattgcatt atttttcgct
     4141 gggcgttttg cctttttgca gcgtttcttt cacctgaccg ataagctgac gacggaaatc
     4201 cccgaggcga ggtttgtcat cgtcaatcca cggcagcggg cggcaaagct ccatagcttt
     4261 aatcccgagt cgtgccgtca gaagtccagc accaattccc tgagctgcgc gggtagacaa
     4321 acgagcagcg agatcttgcg acatccagtc catccccact tcgcgcacca gttcgctggc
     4381 tccggcaaaa gcgatattca gcaataccag cttaaacaga cgcaaacggc tgtaataccc
     4441 cagttcaatg ccatacagcg tggcgatgcg attaattaaa cgcagattgc gccaggcgat
     4501 aaacgccata tcgaccaacg ccagcgggct gaccgcaatc atcaacgttg attccgccgc
     4561 cgaacggctg atttcgcgcc gcgcctgggc atctaaaact ggctggacca aatgcgcata
     4621 caaactgacc acttcacggt cgttttgcgt ttcatggatt gaggcatacc agcgttgcag
     4681 cgctggatga gactgatcaa tacccgcctg ctgcgccagt ttttcgcaaa atgcgcggcc
     4741 tttgcccgtg ccgtggctgt gcaacaaatc gcgcgcttcg tcgcgttcat gggcgcgctg
     4801 tcgcaagcgc cataagcgcc gccactctgt taccacagaa cctacgccag cgccgataat
     4861 caatgcccca gcggcacatc cacccagcgc cacccagtcc tgagtttgcc aggcattcat
     4921 tgtccactgt acaccctgcc cgacaacgct tgcgccaaac agagccagcc cgcccatcac
     4981 cattttgcgc cacaggctgc gtttcggacg taacgctgca tccattaccg cttcaacttg
     5041 cccctcttct tcaggcgctt catcgagcgt ggccggggca aaattttgcg cctgattttc
     5101 gtcaaaggtc tgctgcgccc tgaattttgg attctgatcg acctccagcg gaccgtcgaa
     5161 atcaatacgt ggttttaacg gttcggtcat cgcaatttat ctcctattaa aaattccagc
     5221 gcggcatcaa gacgaatatg cggcagcggt ttgtcgacat ccatcacctg cgggcgaaac
     5281 gcttcaaact ggaacccttg tttgtcccag aacgcctgac cgggcaaacg cgcaggaact
     5341 tcaccgggat aaacggtgag tggcacacca tcgctgagcc ggttaccgcg caatgccgga
     5401 attttctcgc cgttgacgtc aataatgccg ctggtggtcg cctgcaccga ggccagcccc
     5461 aggcaatcca tactaatccc ttcaaacgcc gcattttgcc aggcatcctg aatcagttgt
     5521 tgcagtaatg aaaccatatt ggcgtgctga tcgatggtca catggtccgc tttagtggca
     5581 gcaaacaata gcttatcgat aaccggcgaa aacaaacgcc ggaacaaggt acgctgcccg
     5641 tagtggaaac tttgcatcag ctgcgtcagt gccagacgca tatcattaaa tgcctgtggc
     5701 ccactgttga gaggttgcag gcaatcaacc agcacaatct ggcggtcaaa gcgcagaaaa
     5761 tgattcttat agaacccctt caccaccttc tcgcagtaat aattaaaccg ctcgcgcagc
     5821 attccggcat tggtgtgctt atcggcctgc gccagtttgg actcgcccca ggtatcgaca
     5881 tccggccacg ggaagaattg cagcgcgggc gcacctgcca tatctcctgg caagacaaag
     5941 cgccctggct gaataaagtg cagcccctgc tgtttacagt ggtggagata atcggtccac
     6001 gcggcggcaa tgtctgccag ccggttttcg tcggcaggtg ctagcgggtc cagcccttcg
     6061 cacatcattc gccatttgac cgaccattcg ccgcgctgac cattgagtaa gcccgtcatc
     6121 tggcgtgacc agcttaaata gtcctgcgcc agcatcggca ggtcgagcaa ccattcgccg
     6181 ggataatcca caatctccag atacagcgtg gaggtgtcct tgaagtggcg tagcagcgaa
     6241 tcgttcgatt taaaacgtag cgccaggcgg atttcactga cgccgcgcgt tggcgtcggc
     6301 caggcgggag gatcgccata taactgcgcc agtccttcat catatgtgaa gcgcggaatg
     6361 ccaaagtcac gctgaggaat gcgttttacg cccagcaggc gctcttcacg caccgcgctt
     6421 aacagcggca agcgtgctcc ggcgtgaata ttgagcaact gattgaccat cgcagtgata
     6481 aacgctgttt tgccgctgcg gcttagtccg gttacggcga ggcgcagatg tctgtcgaca
     6541 ccccgattca ccagcgcatt aagttcattt ttaagtcgct tcattgctgt cctgtgctgc
     6601 tctgaagacc agaaaaaaat cattcgtaag gatatgaatg tgggggccag gccattgatt
     6661 atcaactgcc cgtgttgtca tcgacgggcg aactccataa gatgtgcagc attcacgttc
     6721 ggacggagtt cctgacctat gtcccctact atttatgata ttgccagggt tgcaggcgta
     6781 tcaaaatcca ccgtatcacg cgtgctgaat aagcaaacta atatctcccc ggaagcgcgc
     6841 gaaaaagtgt tacgggccat tgaagaatta cagtatcagc caaacaagct ggcccgcgcg
     6901 ctgacctctt cgggttttga tgccattatg gtgatttcta cccgttcgac caaaactacg
     6961 gcgggtaatc cgtttttctc tgaagttttg catgccatca ccgccaaagc tgaagaggaa
     7021 ggtttcgacg tgatattgca gacgtcgcac aatccggcag aagacttaca aaaatgtgaa
     7081 agcaaaatta agcagaaaat gattaaaggc attattatgc taagttctcc agcggatgag
     7141 tcattttttg cccaacttga taaatacgat attcctgtcg tggtgattgg caaagttgaa
     7201 ggtcaatatg cccatgttta ttctgtcgat accgataatt ttggcgacag cattgcgttg
     7261 accgatgcat taattgaaag tgggcatcaa aatattgcct gcctgcatgc tccgcttgat
     7321 gtccatgttt cagtggatcg ggtaaatggt tataagcaga gcctggctgc gcataatatt
     7381 gcagtgcgtg atgaatggat tgttgacggt ggttataccc atgaaacagc actgcaagcc
     7441 gcgagagaat tattaagcca gtcaccgttg cctgaggcgg tgtttgccac tgacagtctg
     7501 aaattaatga gtatttatcg tgcggcggca gagaaaaata ttgctattcc gcagcagtta
     7561 gcggtggtgg gttatagtaa tgaaacgctg tcgtttattt taacacctgc accgggcggc
     7621 atcgatgttc cgacgcagga gttaggtcaa caaagctgcg agttattatt ccgcttaatt
     7681 tctggaaaac cgtcaccaca gaatattacc gttgccacgc atatgacgct gaaataaata
     7741 ccagctacct tgcctgaatg ccggatgcga tgctagcgca tcttatccgg cctacattta
     7801 tagatcggat aaagtactcg cgccgcatcc ggcaatttag cccattatca gaacgagtaa
     7861 tttacgccga cacctgcata atggaattta tcactgtcgc cttcgtcgtg atcctgccac
     7921 tcaaacgcgt attccagcga aacggaaagt ccgttctgga aatcataacc gtaaaataaa
     7981 cctacacggt taaaatcatg gccttcacat tcaatatcat cctgccagtc ccagttactc
     8041 cagcgatcca gcccaatgcg cgtatacggc gtcaccgagt ggttgccgag cgtcagcggc
     8101 aaataggcgc gaatttcttg cgtggaaaac tcaccgttat tgcggctgtc gtccatattg
     8161 aagccgcgct cgagataata gttcactcgc aaggcaaccg tttcgttgaa ggtatattgc
     8221 agacctgt
//
LOCUS       DAJTNA010000168         8100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTNA010000168 DAJTNA010000000
VERSION     DAJTNA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.170"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(574..948)
                     /locus_tag="OO161_003292"
     CDS             complement(574..948)
                     /locus_tag="OO161_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464255.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLDLMDIIDKGFGLLMKKKGIRIEPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(960..1112)
                     /locus_tag="OO161_003293"
     CDS             complement(960..1112)
                     /locus_tag="OO161_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5464256.1"
                     /translation="MDTIDLGNSESLVCGVLPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNSD"
     gene            complement(1302..1709)
                     /locus_tag="OO161_003294"
     CDS             complement(1302..1709)
                     /locus_tag="OO161_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5464257.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNDLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPDPPVALNSALDLSEDELEMLRLYRALPKS
                     EQQAQISELRARVENFNRLFTELLEARKRNKHQ"
     gene            1787..2014
                     /locus_tag="OO161_003295"
     CDS             1787..2014
                     /locus_tag="OO161_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5464258.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            1998..2549
                     /locus_tag="OO161_003296"
     CDS             1998..2549
                     /locus_tag="OO161_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464259.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRKKFTALIPAIMEAIRISDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGLYEYRCTTMG"
     gene            2521..3561
                     /locus_tag="OO161_003297"
     CDS             2521..3561
                     /locus_tag="OO161_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5464260.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNGTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPLVFLDGLSEPIGKYPMTDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            3473..4015
                     /locus_tag="OO161_003298"
     CDS             3473..4015
                     /locus_tag="OO161_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR5464261.1"
                     /translation="MGEVYSNRWTQKNGAAPSKLWIAQIGAMTEQQIRQVCRQCMDRC
                     RAGETWPPDLAEFVALISESGANPFGLTVDAVMEEYRRWRDESWRYDGSDKYPWPQPV
                     LYHICLEMRTRGIERQMTQGELKRLAERQLTKWAKHVGNGMSVPPVRRQLEGAKHPQG
                     PTPIERLKQEYERRKAAGFI"
     gene            4049..4819
                     /locus_tag="OO161_003299"
     CDS             4049..4819
                     /locus_tag="OO161_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR5464262.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            4835..5227
                     /locus_tag="OO161_003300"
     CDS             4835..5227
                     /locus_tag="OO161_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5464263.1"
                     /translation="MAKVFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            5224..5520
                     /locus_tag="OO161_003301"
     CDS             5224..5520
                     /locus_tag="OO161_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCR5464264.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            5517..5978
                     /locus_tag="OO161_003302"
     CDS             5517..5978
                     /locus_tag="OO161_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5464265.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQAIPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLKEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            5956..6312
                     /locus_tag="OO161_003303"
     CDS             5956..6312
                     /locus_tag="OO161_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464266.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARICRATKSRTE"
     gene            6408..6590
                     /locus_tag="OO161_003304"
     CDS             6408..6590
                     /locus_tag="OO161_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464267.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIQDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            6583..6759
                     /locus_tag="OO161_003305"
     CDS             6583..6759
                     /locus_tag="OO161_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464268.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            6756..7115
                     /locus_tag="OO161_003306"
     CDS             6756..7115
                     /locus_tag="OO161_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464269.1"
                     /translation="MSEINYQEGHEKAGQAKPVAWRYRYVKKGVTDFQGKQWVGDWKY
                     VPTKEDCNDRPNYEIQALFTAPPASVTSEGLVKAVRFYEQVRREDPPVETGAWKDAID
                     WVLKEACLVVNTGIKGG"
     gene            7116..7331
                     /locus_tag="OO161_003307"
     CDS             7116..7331
                     /locus_tag="OO161_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464270.1"
                     /translation="MGIAASYTMHLYCDCRQCTDGKYQSPDFGEYMGTSWAGCAKEAR
                     KDGWRISADKTRAFAPGHKILRSNKGE"
     gene            7333..7551
                     /locus_tag="OO161_003308"
     CDS             7333..7551
                     /locus_tag="OO161_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5464271.1"
                     /translation="MPTLFRKEYPRKSRATEFLFLILFIVLMIPISPLILVWGIGKII
                     EPVIELYNDVVWAPFNTLHNKINPYKEN"
     gene            7553..7816
                     /locus_tag="OO161_003309"
     CDS             7553..7816
                     /locus_tag="OO161_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464272.1"
                     /translation="MATLTKKEQAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            7827..7994
                     /locus_tag="OO161_003310"
     CDS             7827..7994
                     /locus_tag="OO161_003310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464273.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
BASE COUNT         2274 a         1689 c         2204 g         1933 t
ORIGIN      
        1 caaccttcag acgtattaac gtttggtaat aacgtttcca ttatcgcgtc ctcaacaatg
       61 aattttgtga tgcggtgcct ggtgcctcca ggtgacgtta accagttaac aattaacgcc
      121 ggatacagag aatccaccca taacactgtt tttggtttta actgttctgc gtgcgctcag
      181 ccgcattcac cacatcacaa aattcacttt aaaaagggcg gcagagcagt cacggagtaa
      241 aactgatacc gccaaacgtc accagaaaat tgataacaga gggcgttgca gcggggttgt
      301 cacttaagcg tatggtcaac ctgacaaccc ggtgtcctca gcgggggaag gaataacccc
      361 gccatactta ccgccgcgcc atttcgcgga ttgccacaac cggaagcgca cgatcgaatt
      421 aaatttaacg acgacctata cagagagact aacttctccg ggcgctttcg tgttatgccc
      481 tgacttttca gggatatatc cttttcagta aactgtcagt gccggattct tatccgtgtc
      541 cggcgcacgc actctacctc acctgtgaat aaattaatga ttaattgata ttttgttgtt
      601 tgattcaact ttcccatcgg atgtgtgatg ctttaaatca caggaattaa tactgcttgc
      661 tgtaaaatga ttttcaaggg gttctattcg aatccctttc tttttcatta acaagccaaa
      721 ccccttatca atgatgtcca ttagatccag gaagtatttt tcatgtaaat cctggttatc
      781 agagagctgc ttctcttcgt acagcccgat aaaggctcgg cgtacgttac cggatatatt
      841 gtcgatggtt tctttttcta cggtactcag gtcaagagtc gccagttgag agcgaaccac
      901 attcgatgcc atttcctgga atggtactgg taaatcttta aattccatcg tcaacctcat
      961 cagtcggagt ttcttgctaa ccagcgacgc gcgccagctt cggttttaaa cgttttgctt
     1021 ttggtatacg tcatggcggt gaatgtgccg tcctggttgg ggagcacacc acataccaga
     1081 gattcgctgt tgccaagatc gatagtatcc atgctgacct cattacccct taacgccggg
     1141 tagcggaact gtttgctgag aacaccgtgc ggtgtcttga tgggtggtaa tttagttttc
     1201 tcatgaatgt tggtcaagca tttttaatga gaaaactcaa catttaatgc aaaataaagc
     1261 caatacattg aaatgtaagg ctttaaaatt tgtgaagggg gttactgatg tttgttacgt
     1321 ttgcgagctt ctagtagctc ggtgaatagg cgattaaaat tctcaacgcg ggcacggagt
     1381 tcgctgattt gtgcttgctg ctctgatttt ggaagtgcgc gatacaatcg caacatctcc
     1441 aactcatctt ccgataagtc taaggcgctg ttgagtgcta ctggtggatc tggtgtttta
     1501 tcctcgtcac caaacagtat ccaagttggt gaacattgca atacctcagc caggcgatgc
     1561 aaattttgcc cgcgcggggc tgtatggtcg ctttcccata gtgaaattga tgagccagat
     1621 acgccagcgg ctttgcttaa atcgttttga cttaaaccaa cctgtttgcg tctttctcta
     1681 attcgttgac ctaaagtttt ctcgttcata tttagatatc ttaataaccc ttgactcgag
     1741 attccttgaa tgattgctat tgagaaaact caactttgga ggggtgatgt ttaaatcaga
     1801 cgtaattaat ttttatggga cgaaagccaa agtagcgaaa gctgctggtg ttgatccatc
     1861 tgctgtttct caatgggggg aactggttcc tgaaggtcgc gcgatgcgcc tgcaagaggc
     1921 atccggcggg gaacttcagt acgaccccaa agtttatgac gaatatcgta aggcaaagcg
     1981 ggcggggcgg ttgaacaatg aaaatcaccc ctgaacaggt ttgtgaggct ctggatgcct
     2041 gggtatgtcg accaggaatg acacaggagc aagcgacgat attaatcacg gaagcattct
     2101 gggctctgaa agaacgcccg aacatcgatg ttcaacgcgt cacgtttaat gatggcgagg
     2161 ttgatcaacg ggcgctgggc gttaaccggg tgaagatatt cgaacgctgg aaagctatcg
     2221 acaccaggga taagcggaaa aaattcacgg cgctgattcc ggcaattatg gaggctatcc
     2281 gaattagtga tttcaggttg tatcgtgaga tcagtgatgg aaaaagcatt acgtacatga
     2341 tcgccggatt aaacaaagaa tatggcgatg tggtggagtc cgggctgctt tttgcggatc
     2401 cagctgttgt ggaacgtgag actgacgagc ttatagaaaa agctattgct ttcaagcatg
     2461 cgtatcgtca gcaatatcaa tattactttg cagataaaca aatgtctgcc aggggtttgt
     2521 atgagtatcg atgcactacg atgggctaaa aaggtgaaaa ccggcagttc atccagtaag
     2581 tctgtattga cctggcttgc tgatatgtgc ggtgccgatt tgtgtgcata cccgtctgta
     2641 tctgcactgg cagaagtaac ggaactaaac aaaaagactg tgcaggacag cttacgacac
     2701 ctgatggaga ttgggttaat tgttgatacc ggtgagagaa aaggcagaac aaagcaaatt
     2761 gtggtgtacc gacttatcgg tgtagaagaa agtgttgccg agcctgaata cacccaaaaa
     2821 cgggagtctt taaaggtggg taaaattggt gctgttaata aaaacagtac cgaaaatggt
     2881 tatgtttcag cacaaaacag acccaaaaac ggaactctta gctgcatgga aaataaccaa
     2941 agacacccaa attttccatc aaagacaccc aaaaacggat cacggaaccc aaaggaaccc
     3001 aaagatctaa accccacaca taacgcacgc gagagtgctc cgaccagtga gcaggaagtt
     3061 ttgtcgttac aggcagcacc ccttgtattc ctggatggcc tgagcgaacc catcggaaaa
     3121 tatccgatga ccgatagctg gtatccgtca cgggattttc gacgacgggc tgcgttgtgg
     3181 gggatggctt tgccggagac agaatttaca cctgctgaac ttgccgcctt ccgggactac
     3241 tgggcagcgg aggggaaagt gtttacgcag attcagtggg agcagaaatt cgcccgtcac
     3301 gtaaatcacg tcagggcgca ggttaaacca gtcagcaagg gggtaaacca tgcagcagca
     3361 ccaggtggca ccgcatcacg ggcagttcag gaaattcggg cagcacgtga gcagtgggaa
     3421 cgtgaaaacg gatttatcag cgatggaaac ggtctggaag ctgtgggaac tcatggggga
     3481 ggtttattcg aaccgctgga cccagaagaa cggggccgca ccttcgaagc tctggattgc
     3541 acagattggc gcgatgactg agcagcaaat ccgacaggtc tgccgccagt gcatggaccg
     3601 ctgccgggcg ggtgaaacat ggcctccgga cctggctgag tttgtggcgc tgatttcaga
     3661 aagcggggcc aatccatttg gcctgacggt ggatgctgtg atggaggagt accgccgctg
     3721 gcgtgatgag tcctggcgat atgacggaag cgacaaatat ccgtggcctc agcctgtgct
     3781 gtaccacatc tgcctcgaaa tgcgtaccag agggattgag cgccagatga cgcagggtga
     3841 gttaaaacga cttgcggaac ggcaactgac gaaatgggca aagcatgttg gtaacgggat
     3901 gagtgttccg ccagtgcgac gacaactgga aggggcgaaa cacccgcaag ggccaacgcc
     3961 aattgaacgg ctgaaacagg aatacgaacg ccggaaggca gctggtttta tttgaatctg
     4021 agaaacgatt ttgtcggagg aaattttaat ggaaaccgta tttgacgcac tgaaagcaat
     4081 gggaaaagcc acatcggtag aactggccgc gcgacttgat atcagtcgtg aagaggttct
     4141 caacgagctg tgggaactca aaagaaatgg cgtcgttgat aaaactggtc acacctggtt
     4201 tctggctggc gaaggtgaat cccgggtaac cgaagagcgg ccagtaaaat ctgaagcaca
     4261 ggatatgctg accggggagg tcgaacaaaa agttaccgca gacatgatga ttgagtttat
     4321 cggtcaggat ggtgctaaaa cgtgtgagga actggcgggt aagttcggcg tcagtactcg
     4381 caaggttgcc tccacgctgg cggtggtaac cgcaacgggg cggctggcac gcgttaatca
     4441 gaacggtaaa tttcgttact gcatgccggg cgataattta ccagcagagc cgaaagccgc
     4501 gctggtaacg gaaagtgatg gtaaggcctt tcctcagcca gcaggtgctg cgttaccagt
     4561 ccgggaagcc gcaacacagg aagaaattaa aacagaaact gtggcggaca ttgtgcagcc
     4621 gttgccatcg tttaccgaaa cgcaagcaga tgagctgatt tttccgtccc ttcgcagggc
     4681 aaacctggcg ctgcgcaggg cgaaaagtga tgttcagaag tgggagcgag tctgcgccgc
     4741 gctgcgggag ctgaacaagc accgggatat tgttcgacag attactgatt cttcccgccg
     4801 tgttgtatcg gaaaagtgat tgccggaggc gcttatggca aaagtattta caccagaaga
     4861 gcgggaagaa gtgaaggcgc gcattgtgga attcgtgcgc ctgagcggac gagaaacttt
     4921 tcgacaactg gcagataaaa cgggtgtcag taagaccgct attcgtcgtt tatctggtgc
     4981 gcttgcggcc agtggtgatg tctggctctc tggttgcggg gtatttccat cagagcaggc
     5041 gtatcgcgta tggcgtaaga caccggagaa ggctgctgac ccgacactga ttcgaaagtt
     5101 acctgacgga gaaatacgtc gttacaacag acggcagaac ataatttgtc gtgagtgccg
     5161 ccagagcgaa gttatgcagc gtgtgctggc gttctatcgg ggaaactttc aggaggtgat
     5221 ggagtgaggg tcagagttta tattgccggt ccaatgacgg gatatgaaaa tttcaaccgt
     5281 gaggcgtttc acaaggcgga agaggaactg aaacgggaag ggcataccgt cttaaacccg
     5341 gcagtacttc cggacgggct gacacagccg cactacatgg atatttgcat ggcaatgatt
     5401 cgttgtgtgg atgcgattta catgctgaat ggctggcagc ggtcagcggg cgctaaggca
     5461 gagctggcac tggcggagaa actggggcat gcagtgattt atcaggaggt ggctcaatga
     5521 gagaggttaa ctatgaggcg cttcgtgagg cagcacaaaa ctatcagtcg acgctggcgt
     5581 ggtatcaggc tatcccggac agcccaaatg ctgaacggga ttgtgatgcg gctcttgctg
     5641 cgtttaagcg tcacatccgt catcgggaag cggatattat cgctgatttg ctggatggac
     5701 tggaagaagc aaaatcacaa ctcaaagagc agcgtgagta ttacgaaggc gttatctctg
     5761 atgggagcaa gcgtattgct gaactggaag cgcgggaagt tcaattaccg actcgctacg
     5821 accttcgata tggacacccg ataaatgcag atgagcgaca tgtcatgata cctaaagaaa
     5881 atggcagttg gctttacctg attgacctag aacacgcatt acgcgtcgct ggcattcgca
     5941 tcaaaggaga ggagcatgga aataaaacca gaggatgagt taagcaatat cgttttattt
     6001 ccggtaaaag aggatgaccc tcgtaatcag gttaattttc tttatgagcc atcggaaaga
     6061 ccatattgtc atcacgcctc tgttcgggtt gacgaaaaag agcgtcaggt ccgctgtaaa
     6121 atctgcggtg cagttgtgga gccatttgac tggatgctct ctgtggcgaa aagagaaacc
     6181 agactggcag atgatgtaag gctattgcgc caggaggaac aggaaaggcg gaaaaatata
     6241 gaaaagttaa ttcagattga gcgtaacgcg aaagcgcgga tatgcagggc gacaaaatcc
     6301 agaactgaat aattaaattt agcactgtaa ataaaatcaa atccttaacc ggagggattt
     6361 ctgcaccctc agaacatcag gaggccgtcc gaaagggcgg tagtgaaatg cgaaaattca
     6421 aaataattat tgaaacggga atagccggtg gagatttcga ggatgaattc gaagtggatg
     6481 atgatgcaac accagatgaa atacaggatg aagctaaaga tattttcttt aactactgca
     6541 attactcata ccacgaaata aaagacgaag aggaagaaca aaatggctga ttttggttca
     6601 actaaatata acgccagttt tgaagaatgg catgaactgt taatggatta tgcagagtta
     6661 cgcggtggaa gtgccgctga tgctgaagca tggcgtgatg attatgaagc agggaaaaca
     6721 ccggtcgaag catattgtga tgagtggggc gatgaatgag cgagattaat tatcaggaag
     6781 ggcatgaaaa ggcagggcag gcaaaaccag tggcatggcg atatcgctac gtgaaaaaag
     6841 gcgttacaga ctttcagggg aagcagtggg ttggtgactg gaaatatgtc ccgacaaaag
     6901 aggattgtaa cgacagacca aactatgaaa ttcaggcgtt attcacggcc ccgccagctt
     6961 cggtgacatc agaaggactg gttaaagctg tgcgctttta tgaacaggta cggcgtgagg
     7021 atccgccagt tgaaaccgga gcatggaaag acgctattga ctgggtactc aaggaggcct
     7081 gcctggttgt aaacacgggc atcaaaggag gctgaatggg tattgccgca agttatacca
     7141 tgcatctcta ttgtgattgc cgccagtgta cggatggcaa atatcagtcg ccagattttg
     7201 gtgagtatat gggtacgtca tgggctggct gtgcaaaaga ggcacgtaaa gatgggtggc
     7261 gaataagtgc cgacaaaacg cgtgcttttg cgccagggca taaaattttg aggagcaaca
     7321 aaggagagtg atgtgcctac attattcaga aaagaatatc cgcgaaaaag tagagcgaca
     7381 gaatttttgt ttctcattct gtttatcgtg ttgatgatac cgatatcccc gttaattctg
     7441 gtatggggaa tcggaaaaat aattgagcca gttattgaat tgtataacga cgtggtatgg
     7501 gctccgttca acacactgca caataaaatt aatccgtata aggaaaactg atatggcaac
     7561 tttgacaaaa aaagaacagg catggttgaa cgaattacag gacgttcttg atcgctgtcc
     7621 atcaccgaaa aaaattggtt tttacaccat tggcgataaa agcatttacc tgtatgacct
     7681 gcgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcaatgg attggtgtgt
     7741 tgctgtccat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
     7801 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
     7861 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
     7921 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
     7981 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
     8041 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
//
LOCUS       DAJTNA010000169         7992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTNA010000169 DAJTNA010000000
VERSION     DAJTNA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.171"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..190
                     /locus_tag="OO161_003311"
     CDS             <1..190
                     /locus_tag="OO161_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464274.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="OO161_003312"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="OO161_003312"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5464275.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="OO161_003313"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="OO161_003313"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5464276.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO161_003314"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO161_003314"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5464277.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /gene="dgkA"
                     /locus_tag="OO161_003315"
     CDS             4544..4912
                     /gene="dgkA"
                     /locus_tag="OO161_003315"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5464278.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="OO161_003316"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="OO161_003316"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5464279.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5703..7028
                     /gene="dinF"
                     /locus_tag="OO161_003317"
     CDS             5703..7028
                     /gene="dinF"
                     /locus_tag="OO161_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5464280.1"
                     /translation="MAFLTSSDKALWHLALPMIFFNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7144..7353
                     /locus_tag="OO161_003318"
     CDS             7144..7353
                     /locus_tag="OO161_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5464281.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO161_003319"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO161_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5464282.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKSR"
BASE COUNT         1808 a         1965 c         2195 g         2024 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattcc acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tccccagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttactagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcaa
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttgttcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tcttcaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcgct ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gactgttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cgatttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gt
//
LOCUS       DAJTNA010000170         7952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTNA010000170 DAJTNA010000000
VERSION     DAJTNA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7952
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.172"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(159..890)
                     /gene="dnaQ"
                     /locus_tag="OO161_003320"
     CDS             complement(159..890)
                     /gene="dnaQ"
                     /locus_tag="OO161_003320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5464283.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            955..1422
                     /gene="rnhA"
                     /locus_tag="OO161_003321"
     CDS             955..1422
                     /gene="rnhA"
                     /locus_tag="OO161_003321"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5464284.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1419..2141)
                     /locus_tag="OO161_003322"
     CDS             complement(1419..2141)
                     /locus_tag="OO161_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5464285.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2175..2930
                     /gene="gloB"
                     /locus_tag="OO161_003323"
     CDS             2175..2930
                     /gene="gloB"
                     /locus_tag="OO161_003323"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5464286.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3002..4360
                     /gene="mltD"
                     /locus_tag="OO161_003324"
     CDS             3002..4360
                     /gene="mltD"
                     /locus_tag="OO161_003324"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5464287.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4409..5005)
                     /locus_tag="OO161_003325"
                     /pseudo
     CDS             complement(4409..5005)
                     /locus_tag="OO161_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(5036..5836)
                     /locus_tag="OO161_003326"
     CDS             complement(5036..5836)
                     /locus_tag="OO161_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5464288.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6077..6991
                     /locus_tag="OO161_003327"
     CDS             6077..6991
                     /locus_tag="OO161_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5464289.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSGHVKTAPGGAVREA"
     gene            complement(6988..7791)
                     /gene="dkgB"
                     /locus_tag="OO161_003328"
                     /pseudo
     CDS             complement(6988..7791)
                     /gene="dkgB"
                     /locus_tag="OO161_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
BASE COUNT         2119 a         1913 c         1931 g         1989 t
ORIGIN      
        1 attctaaccg actgaactac cgctccgcgt tgtgttccgt tgggcacgag gcgaatatta
       61 cggattgcct cacccttcgt caacggtttt tctcatcttt tgaatcgttt gctgcaaaaa
      121 tcgcccaagc cgctattttt agcgcctttt acaggtattt atgcccgcca gaggcagctt
      181 ccgcccttct tctccaccag atcaagacgg gcttcatgag ctgcaagctc ttcatctgtc
      241 gcaaaaacaa cgcgtaactt acttgcctga cgtacaatgc gctgaattgt tgcttcacct
      301 tgttgctgct gtgtctctcc ttccatcgca aaagccatcg acgtttgacc accggtcatc
      361 gccagataaa cttccgcaag gatctgtgca tcgagtaatg ccccgtgtag cgttcgttta
      421 ctgttatcta tttcgtagcg ggcacataac gcatcgaggc tgttgcgctt accaggaaac
      481 attttcctcg ccaccgcaag gctatcggtg accttacaga aggtattggt cttcggaata
      541 tcgcgcttaa gcaacgaaaa ctcgtagtcc ataaagccga tatcgaacgc tgcgttatgg
      601 atcaccaact ccgcgccgcg gatatagtcc atgaactcat cggctacttc ggcaaacgtg
      661 ggcttatcga gcaaaaattc atcggcaata ccatgtacgc caaaggcttc cggatccacc
      721 agccgatcgg gtttgagata aacatggaag ttattgcccg tcaggcgacg gttcaccact
      781 tcaacggcac caatctcaat gatcttgtgg ccttcatagt gcgcaccaat ctggttcata
      841 ccggtggttt cggtatcgag aacgatctgg cgtgtaattg cagtgctcat agcggtcatt
      901 tatgtcagac ttatcgtttt acagttcgat tcaattacag gaagtctacc agagatgctt
      961 aaacaggtag aaattttcac cgatggttcg tgtctgggca atccaggacc tgggggttac
     1021 ggcgctattt tacgctatcg cggacgcgag aaaaccttta gcgcaggcta cacccgcacc
     1081 accaacaacc gtatggagtt gatggccgct attgtcgcgc tggaggcgtt aaaagaacat
     1141 tgcgaagtca ttttgagtac cgacagccag tatgtccgcc agggtatcac ccagtggatc
     1201 cataactgga aaaaacgtgg ctggaaaacc gcagacaaaa aaccagtaaa aaatgtcgat
     1261 ctctggcaac gtcttgatgc tgccctaggg caacatcaaa tcaaatggga atgggttaaa
     1321 ggccatgccg gacacccgga aaacgaacgc tgtgatgaac tggctcgtgc tgcggcaatg
     1381 aatcccacac tggaagatac gggctaccaa gtcgaagttt aagcttgtgg tttacgacat
     1441 tgccgggtgg ctccaaccgc ttggcgaatt cgtggcttgt ttttactctg tttcatcggg
     1501 tttagcgtta aaggaatagt ccgtttccgg gcaacaataa gttgtaagca accaagcgca
     1561 ggaatatgcg cattcaatag tttacctccg tgtttgttcc acgggagaac gtggaaacgg
     1621 ctggcgtgca gcacttcaaa attcaacaaa gagagccagt ccagttgccg catcagagta
     1681 aacatccggc tgttataggg cgaggttttt cgcaataccg gcacaagttt gcgtaatccc
     1741 attaaactaa tgggattgaa gccactaatc accagccagc catcatcaat caatacccga
     1801 tcggtttcac gcaataaacg atgcggatcg gtgcaccacg gcaatgtatg tgccagtaga
     1861 caaacatcaa cggatttatc ggcaaaagga agatgaagtg gatccgcctg cacctggacg
     1921 ggcattcctt gcgcagaaac attcacttga tgagaaaccg cgcacgcttc gcaattgatt
     1981 tctgcgctta aattgccaat cttaagcaga tgaaaaccat acattttagt gaaccacggg
     2041 ttgagctggc gctccagcgc cttgcgataa agctctcccc agggcaaatc gccccagcga
     2101 tcaggagcca cgacagtttg agggactctt gccggtttca tcacaacctt ccgtttcaca
     2161 ctaagaggta atctatgaat cttaacagta ttcccgcctt tgatgacaat tacatctggg
     2221 ttttgaatga tgaagcaggt cgctgcctga ttgtcgatcc cggagacgca gagccagtat
     2281 taaacgccat tgccgccaat aactggcaac cggaggccat atttctcacc caccatcatc
     2341 acgatcacgt tggcggcgta aaagaactgg tggaaaagtt tccgcaaatt gtggtgtatg
     2401 gtccacaaga gacacaagat aagggaacaa cacaggtagt caaagatggc gaaactgcct
     2461 tcgttttggg gcatgaattt agtgtaattg ccacaccggg tcacacttta ggacatatct
     2521 gttacttcag taaaccttat ctattttgcg gcgacacgct gttttctggt gggtgtggta
     2581 ggttgtttga agggacagca tcacaaatgt atcaatcact taataagtta agtgcgttac
     2641 ctgacgatac attggtatgt tgtgctcatg aatatacgtt atcaaatatg aagtttgctt
     2701 tgagtattct tccgcacgat ttgtccataa atgattatta tcgtaaagtt aaggagttac
     2761 gggcaaaaaa tcaaataaca ctacccgtaa ttctgaaaaa tgagcggcaa attaatgttt
     2821 ttttaagaac ggaagatatt gatttaatta atgtaattaa tgaagaaaca ttattgcaac
     2881 aacctgaaga gcgttttgca tggttaaggt caaagaaaga taggttctga taaaactttc
     2941 ttgtcatcgg ctccgttcgc cgttatgatc ggtcgtcttt taagcaacta ttgacacaca
     3001 catgaaggca aaagcgatat tactcgcctc tgtcctgctc gtgggttgcc agagtaccgg
     3061 caacgttcaa cagcacgcac agagcctttc tgcagctggt caaggggaag cagcaaagtt
     3121 tacaagtcag gcacgatgga tggacgatgg gacgtctatc gcgccagatg gtgacttgtg
     3181 ggctttcatt ggcgacgagc taaagatggg aattccggaa aatgaccgga ttcgcgaaca
     3241 gaaacagaaa tatttacgca ataagagcta tctccacgat gtaactttac gggcagagcc
     3301 gtatatgtac tggatagcag ggcaagttaa aaaacgtaac atgcctatgg aactggtact
     3361 actacccata gtggagagcg cttttgatcc tcacgcaacg tctggcgcca atgccgcagg
     3421 catctggcag atcattccga gcacggggcg caattatggt ttgaaacaga cccgcaatta
     3481 tgacgcgcgt cgcgatgttg ttgcttcaac aactgccgcg ctgaacatga tgcagcgtct
     3541 gaacaagatg tttgacggcg actggcttct gaccgtagcg gcttataaca gcggcgaagg
     3601 tcgggtcatg aaggcaatta aaacgaacaa agcgcgtggg aaatccacgg acttctggtc
     3661 gttaccgttg ccgcaggaaa cgaagcagta cgtgcctaaa atgctggcat tgagtgatat
     3721 tctcaaaaac agcaagcgtt atggcgtacg tctgccaacg accgatgaaa gccgtgctct
     3781 ggcgcgtgtg cacctgagca gcccggttga aatggcgaag gttgcagata tggcggggat
     3841 ttccgtcagc aagctgaaga cattcaacgc tggcgtaaaa ggctccacgc tgggcgcaag
     3901 tggcccgcag tacgtgatgg tgccaaagaa gcatgcagat caactgcgtg attctctcgc
     3961 ttcaggcgaa attgctgctg tacagtcgac gctggttgcc gacaatacgc cgcttaacag
     4021 ccgtgtttac accgtacgct ctggcgacac gctttcaagc atcgcttcac gtctcggcgt
     4081 aagcaccaaa gatttgcagc agtggaacaa actgcgcgga tctaagctga agccaggcca
     4141 aagtttgacg attggtgcag gcagtagcgc acagcggttg gcaaacaaca gcgatagcat
     4201 tacgtatcgt gtgcgcaaag gcgattcgct ttcaagcatt gctaaacgcc acggcgtgaa
     4261 catcaaagat gtgatgcgct ggaacagcga cactgcgaat ctgcaaccag gcgataagct
     4321 gacgttgttt gtgaaaaaca acaacatgcc agattcctga caaaccagat aataaaaaag
     4381 gcaccgattc ccccggtgcc ttttttattt atgccgcttt atgtgcttct accatgatga
     4441 tatcactggt gaaagagcca tcagtctgca aggaaaaata cgttctcacc tctgtcgatg
     4501 cgctctgttg gtaaatgcga atagcgtcta ctaacgcttc tggcgtacgc attctcacga
     4561 cccatgaaga aaattccagc ggtaacttat ctgtaattaa attatcaact atcagattgg
     4621 cttcattgat taacgtcaac cacgcaccgc tggcgtagtt tcgtacgtga gaggtatcgc
     4681 gtaatgcttc taccgcctgt aaccagatgt cgcgcactgg gtgacccgga gacattacat
     4741 ccatcacaat cagcctaccg ccaggtttca atatcctatt cacttctcgc agtgctgcac
     4801 caacatcatg ccagtgatgg gcagaataac ggctgataac aatatcaaat gcgttatcgg
     4861 caaatggcag acttgcggca tatccctggc gggtggtgat atttttcagt tgccgggctt
     4921 cggcagcttg tgccacgaca tccagcatgt gggccgataa gtcatacgcc accaccgcgc
     4981 tcacgttttg cgcagcgcca aacgttggtc tgccctgtgg cagacctgac ataccttatt
     5041 tatcaggctt gccgggactg aattcaacga gtagcggatt gtgatcggaa gcgcgcgtaa
     5101 ccagtacaga agcttcactg acgttcagac cacggtagaa aacaaaatcg agcgggcgac
     5161 caaacgcccg acggcgctga tcatcggtaa aacgcacctg gcgcagcgac atttcccgcg
     5221 caaagcgata taacgcgttc atccttctac ggctccaggc attgaaatct cccgccataa
     5281 tgaccgggcc gctgtggtga gctatctgat caccaatagg aagtaactgc ttactataga
     5341 catccacgcc cagactgaag ttgacggcgt gtatattaac caccatcaac aggcgggtgt
     5401 caggtaatgg atagaccgtc accagtgccg acttcgccag acgcaaaatg ggttctcgtt
     5461 cacgtaacgg gcagcaatac actggatgtg ccgccgaaag ggtcattacg ccagaaggat
     5521 gttgtggcag cacgaaagcg ggtacctgat cggcggcaag atagttagcg gtcgcaaact
     5581 gtactaactc tggcgttgtc tgcgcttcct gcaataacac cagatgtgca tctttgccgt
     5641 agttctttaa taccgacaac cattcagcgc gttgctgttt gtatatgttc cacaccagga
     5701 tccgaatacg ctcttcggta cttaacggtt ccccaggtgg taatgcctgg ccgatgctcg
     5761 caaaagaccc cggcggtaag atcctttccg caggttgtcc ggcaacatag cgcatggcat
     5821 aggtgttttt tcgcactttt gactttgaaa cctctattac gtcaaccctt ccgcactcgg
     5881 gagggctcta ctattatagg gattttagct cactctttca acgagcaatt gtatattgtt
     5941 atgtaagcaa gtgcttacac cggtcagaat tttgctcatt atggagaaca ttaaatcgca
     6001 tgacatatca cgcatcctga tggtttaatg acaaataaat tcaaaatatc tcttctattt
     6061 tgcaacagga gcaaaaatga aagccacgtc ggaagaactc gccatttttg tttcggtcgt
     6121 cgaaagcggc agctttagcc gggcagcgga acaattaggg caagcaaact cagcggtaag
     6181 ccgggcggtg aaaaagctgg agatgaaact tggcgttagc ctgcttaatc ggaccacacg
     6241 acaacttagc ctgacggaag aaggcgagcg ttattttcgt cgcgtacagt caattttgca
     6301 ggagatggca gcggcagaat cagaaattat ggagacgcgt aatacaccgc gtggactgtt
     6361 acggatcgat gccgcaactc cagtggtgct gcactttctg atgccgttaa ttaagccttt
     6421 ccgtgaacgc tatccggaag tcactttgtc gctagtctcc tccgaaacga ttattaattt
     6481 gatcgaaaga aaagtggatg tcgcgatacg cgctggtacg ttaacggatt ccagcttacg
     6541 tgccaggccg ttatttaaca gttatcgaaa aattatcgcc tcccccgatt atatttcccg
     6601 ctacgggaag ccagaaacga tcgacgattt aaagcaacat gtttgcctgg gattcactga
     6661 acccgcttcc ctcaatacct ggccgatagc ctgtagtgat ggacaattac atgaggtgaa
     6721 atacggtttg tcatccaata gtggggaaac actgaaacag ctttgcctga gtggcaacgg
     6781 gattgcgtgt ttgtccgact acatgatcga cagagaaatc gctcgcggag aattggtgga
     6841 gttaatggca gataaagtgt tgccagtgga aatgccattc agtgccgtct attacagcga
     6901 ccgtgcggta agtacgcgca tccgggcttt tatcgatttc cttagcgggc atgtaaaaac
     6961 agctcccgga ggagctgtca gagaggctta atcccattca ggagccagac cttccgggct
     7021 aaccaggcgg tcgttgcaat ccagtgcggc gatcgctttt ttatcttcgg catcaagctg
     7081 taaattttgt gccttaagat tactttccag gtttttacgt ttagtagaag aaggaattac
     7141 tgagtaacct tcccccatag cccacgccag aatcacttgt gccggagtcg cattgtgttt
     7201 agctgcgata cgagcaataa cctcatcttt cagggcctta ccatacgcca gcgtcatata
     7261 ggaagtaata tggatgccgt gctgtttagc ccaggcaacc actttacggt tttgcagata
     7321 aggagagagt tcaatctggt tagtagcgat gttttcagcg ccaacagcag caatcgcctt
     7381 ttccatcaat gggatcgtga agttggaaat accgatctca cgcgtcagcc cttatttttt
     7441 ggcttccagc agcgcctgca taaactcttc aacagagact tcatcgtttg gtgacggcca
     7501 gtggattagc gtcagatcaa cataatcggt acgcaatttt tgcaggctct ctttcagact
     7561 tgggatcaat ttgtctttgc tgagattttc aatccagatt ttagtggtga tgtagagttc
     7621 atgacgtggc acgccacttt ctgcaatcgc ctgacctact gcggcttcgt tatcatagat
     7681 ttgtgcggta tcaattgcgc gataaccaag ttcaagcgcc gttttcacag atgaaataac
     7741 aacgtcgtct ttcagacgga aagtacctaa accaaatgca gggatagcca taatatgcct
     7801 cttttaatgc tattttcgtt aactgaggtg attatgggct tcagactgtt gaggaaaaaa
     7861 aagcgtaaaa tgcagaggat ttttgcgatt ctggcaataa tagatataca gacgaaaaaa
     7921 aacctcgagc gttaactcga ggcttcttaa ta
//
LOCUS       DAJTNA010000171         7833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTNA010000171 DAJTNA010000000
VERSION     DAJTNA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.173"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            608..1993
                     /gene="dcuC"
                     /locus_tag="OO161_003329"
     CDS             608..1993
                     /gene="dcuC"
                     /locus_tag="OO161_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5464290.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTILAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(2034..2714)
                     /gene="dpiA"
                     /locus_tag="OO161_003330"
                     /gene_synonym="citB"
     CDS             complement(2034..2714)
                     /gene="dpiA"
                     /locus_tag="OO161_003330"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR5464291.1"
                     /translation="MTAPLTLLIVEDKTPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2683..4341)
                     /gene="dpiB"
                     /locus_tag="OO161_003331"
     CDS             complement(2683..4341)
                     /gene="dpiB"
                     /locus_tag="OO161_003331"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCR5464292.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRMATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPLGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4744..5802
                     /gene="citC"
                     /locus_tag="OO161_003332"
     CDS             4744..5802
                     /gene="citC"
                     /locus_tag="OO161_003332"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5464293.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSMHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5839..6570
                     /gene="rna"
                     /locus_tag="OO161_003333"
                     /pseudo
     CDS             5839..6570
                     /gene="rna"
                     /locus_tag="OO161_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
     gene            6800..7210
                     /gene="rnk"
                     /locus_tag="OO161_003334"
     CDS             6800..7210
                     /gene="rnk"
                     /locus_tag="OO161_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5464294.1"
                     /translation="MSRPTIIINDLDAERIDILLEHPAYAGLPIADSLNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(7441..>7833)
                     /locus_tag="OO161_003335"
     CDS             complement(7441..>7833)
                     /locus_tag="OO161_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464295.1"
                     /translation="LEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKG
                     LTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKV
                     ILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2015 a         1997 c         1747 g         2074 t
ORIGIN      
        1 catatttact cacgttcatt tgtgaccata aaacatttat caaaaatcta ctactagcat
       61 agcaaagcta caattaacat aaccttaata gacccaacat aaagaataat ctgaatagct
      121 gctctattta tttccgcttg ttcaagttaa atatttccgc gcatcgatta aagatgatgc
      181 gtcgttttct gcgatgggaa tagtcaaaaa agaaaaacca agtctttttt gatgacaaaa
      241 tgcaatcaag gaaaaattat tttatttttt aacgagatac aacaatcatc ttaacgaagt
      301 atataatata tatcgtcaac ctatgctcat gttagattat tttttgagcg cacagccgca
      361 gctaacacca ttgcaattaa caaatttgca tcaatccacc atcaatttgc acacattatt
      421 atgtgataat tgccaaccgc taaatatgcg ttttgttatc tatgtataaa aacagcaact
      481 tcaatgtctt aatggcagtt tttcttgatt ttaatcagca ttcatcgcca atttattggg
      541 catatttttt ccttaatctt taggaatttt ttattattta ctttggggcc tggagacagg
      601 aaaaattatg ctgacattca ttgaactcct tattggggtt gtggttattg tgggtgtagc
      661 tcgctacatc attaaagggt attccgccac tggtgtgtta tttgtcggtg gcctgttatt
      721 gctgattatc agtgccatta tggggcacaa agtgttaccg tccagccagg cttcaacagg
      781 ctacagcgcc acggatatcg ttgaatacgt taaaatatta ctaatgagcc gcggcggcga
      841 cctcggcatg atgattatga tgctgtgtgg atttgccgct tacatgaccc atatcggcgc
      901 gaatgatatg gtggtcaagc tggcgtcaaa accattgcag tatattaact ccccttacct
      961 gctgatgatt gccgcctatt ttgtcgcctg tctgatgtct ctggccgtct cttccgcaac
     1021 cggtctgggt gttttgctga tggcaaccct atttccggtg atggtaaacg ttggtatcag
     1081 tcgtggcgca gctgctgcca tttgtgcctc cccggcggcg attattctcg caccgacttc
     1141 aggggatgtg gtgctggcgg cgcaagcttc cgaaatgtcg ctgattgact tcgccttcaa
     1201 aacgacgctg cctatctcaa ttgctgcaat tatcggcatg gcgatcgccc acttcttctg
     1261 gcaacgttat ctggataaaa aagagcacat ctctcatgaa atgttagatg tcagtgaaat
     1321 caccaccact gctcctgcgt tttatgccat tttgccgttc acgccgatca tcggtgtact
     1381 gatttttgac ggtaaatggg gtccgcaatt acacatcatc actattctgg tgatttgtat
     1441 gctgattgcc tccattctgg agttcctccg cagctttaat acccagaaag ttttctctgg
     1501 tctggaagtg gcttatcgcg ggatggcaga tgcgtttgct aacgtggtga tgctgctggt
     1561 tgccgctggg gtattcgctc aggggcttag caccatcggc tttattcaaa gtctgatttc
     1621 tatcgctacc tcgtttggtt cggcgagtat catcctgatg ctggtattgg tgattctgac
     1681 aatactggcg gcagtcacga ccggttcagg caatgcgccg ttttatgcgt ttgttgagat
     1741 gatcccgaaa ctggcgcact cttccggcat taacccggcg tatttgacta tcccgatgct
     1801 gcaggcgtca aaccttggcc gtaccctttc gcccgtttct ggcgtagtcg ttgcggttgc
     1861 cgggatggcg aagatctcgc cgtttgaagt cgtaaaacgc acctcggtac cggtgcttgt
     1921 tggtttggtg attgttatcg ttgctacaga gctgatggtg ccaggaacgg cagcagcggt
     1981 cacaggcaag taaatagtaa tgccggaggc aagtttcctc cggctttatc atgtcaccca
     2041 ctgtggtata tgcgttgtgg tctgccaact ttgccgtgaa caatttcagc aataatcaga
     2101 tggcggctgg cgcaatattc aagataacgt ctggcagtgg tgcggctgat ggtcagtgcc
     2161 tgcgccaccg tttctgccgt atgttgcaca ccaggctctt taaacagttt tcgcaccgcg
     2221 tttagcgtca agggatcaat gccggtcggc agttcgtctt taggttcacc gcgagcatag
     2281 gcattaaaca tctcatcaat ttgcttctgg ctggcgctat caatactttc cagcatatgt
     2341 ttacgctggc ggaaacgggt tagcgtttgc cccagccgtt cataggcaat gggcttaatg
     2401 agataatcaa atacaccaca acgtacggct tcagacaccg tttccatatc gctggctgca
     2461 gtggtaaaca ccacgtcgcc gggataatgc gcctgcacca gttcatgcaa taaattaatc
     2521 cctctgccgt caggaagata gttatcgagc aagattagcc ccggcttaaa acgctcgatc
     2581 atcattcggg cctgcgccag gtttcccgcc agtaatatct gactgaatcc gggaatgtga
     2641 cgaatatatt ccgcatgcat ctctgccagc ggcgttttgt cctcaacgat caatagggtt
     2701 aatggagctg tcattaggtt tcactttcgg aatatagatt gaaaataagg taccgcaggg
     2761 atcattatct tcgagagtga taacaccacc gcagcgcgtt acgtagctgg caatcaagta
     2821 caacccaatg ccatgttcac cgggctcgtc agcacgcgta ctgaccccct gctcaaatat
     2881 tttgtctcgt agagactctg gaacgccgca gccctgatcg gcgacttcaa tcaccacatc
     2941 atcgccttca tcgctgagga ataattcaac gatcttgttt ccttcatcgc tacgcaggct
     3001 ggcttcgaag gcgttatcaa gtaaattgcc cacaatggct gcaaactcgg tgctatccag
     3061 tcctagcggc agttgcgaaa gctggctacc ggggacaatg atcattttta gccccagttc
     3121 ccgggcgcgc tgcactttac caaaaagcag ccccgccacc tggcgatcgg caaacgcctc
     3181 gcgcaggctg tcaataagct gttgctgggc ctgagactcc ccctgcacca tcgccagcac
     3241 gcgatcatac tctttcatct gcaacagacc attgagcgtc gacatccaat tgagatgctc
     3301 gtgtcgcaat gtacgaaggc tctcaacgta ttgttttatt tgcgtcagtt gcgcattgag
     3361 ggtggaaatt tcgtctttac tgcgaaagct gataatggcc cccagcaaat catcacctga
     3421 acgaatagct tcccgattgg caataacgct cagaccgtta aagttagcca ccacatcctg
     3481 acgtttttca tcaatctgtt cggtaaagaa atcggcgggc ctgaccactt cagcaatggg
     3541 tttacccaac cattgccgtc cgggggaact cagccccagc atctttcttg cgttacgatt
     3601 gatggcggta atgtaaccgt gcggatccac cgcaatcagc ccttcataaa ccgaactaaa
     3661 cagcgcctct tgctgacgga ccacgcgtgc gatttgcttt ggctccatgc ccatcatctg
     3721 ccgacggata tgcgcggcca ggaaccacga cagcaacatc agaatcccta acagcacgac
     3781 aaacacacct gccatcggta ataaaaactc agcccgccag ctatcgattt tactcaccag
     3841 gtagccaatc gacaccacgc cgatgacttt tccatcgtca tcaaagattg gcgttttggc
     3901 gcgcatcgcc atccccattg accctttccc ggtaatgaag tagctctccc ctttctccag
     3961 cgcgccctgt ttggtgaact gcataggata accaattttc tccggattag gatggtaaag
     4021 gcggatcgag tgccggtcgc caatcaccac ataatcaaaa tcggtatctc tttgtaattt
     4081 gttagcgatg gtcgccatcc gtttgtagtc acgcgttttc accgcagaga tgacactgtc
     4141 attggaggca ataattttcg cctgattcat tgccatgtcg cgtacatgaa gcgttaaata
     4201 gtcctcaaaa ctggccgtaa aatattgcgc caaggctgca atgacaaata ttgagaacac
     4261 cagaatcagc aaaaagatac gcagcggaaa tgccagtctt tggaaaaatg caaactgttt
     4321 attctcgtta agctgcaaca ttgttttcct tgcgggttgt cccgagcctc tttgacagat
     4381 gtaattaaaa tgttgaatca aaatacatag atattaatta tgatcgtggc aaattttatt
     4441 cattaaatca attaaatcac ttaaacctgc aggtcatttc gtaattacat taattaaaaa
     4501 acttaaacca ttaaataaat aaataaataa ataaataaat aaataaaaaa ccactaactc
     4561 tatgtgaaat aaatcaaaat ttcacgccga aatactcctt aggatgtata gcgaaaggag
     4621 aaaaagatat acctcgatca cccccctttc tcccaagtga aaataaaagg ttatcagttt
     4681 gcaacattga acaacattcg ttgcaaatcg ataacaacat gcaccttcag gatactattt
     4741 attatgttcg gcaatgatat tttcacccgc gtaaaacgtt cagaaaataa aaaaatggcg
     4801 gaaatcgccc aattcctgca tgaaaatgat ttgagcgttg acaccacagt cgaagtattt
     4861 attaccgtaa cccgcgatga aaagcttatc gcgtgcggtg gaattgccgg aaatattatt
     4921 aaatgcgttg ctatcagtga atccgttcgc ggtgaaggac tggcgctgac attagccact
     4981 gaactgataa acctcgccta tgagcggcac agcatgcatc tgtttattta taccaaaacc
     5041 gaatacgagg cgctgttccg ccagtgcggt ttttccacgc tgaccagcgt acccggcgtg
     5101 atggtgctga tggaaaacag cgccacacga ctgaaacgct atgccgaatc gctgaaaaaa
     5161 tttcgtcatc cagggaacaa gattggctgc attgtgatga acgccaatcc ctttacgaat
     5221 ggtcaccgtt atctgattca acaagctgcg gcacagtgcg actggttgca tctgttttta
     5281 gtcaaagaag attcttcacg cttcccctat gaagaccggc tggatctggt gttaaaaggc
     5341 accgccgata ttccacgcct gactgtgcat cgcggctccg aatacatcat ctcccgcgct
     5401 acgttccctt gctacttcat taaagaacag agcgtcatta accattgtta caccgaaatt
     5461 gatctgaaga ttttccgtca gtacctcgct cccgcactgg gtgtaactca ccgctttgtc
     5521 ggtactgaac ccttttgtcg cgttaccgcc cagtacaacc aggatatgcg ctactggctg
     5581 aaaacgccga ctatctccgc accgcccatc gaactggttg aaattgagcg gctgcgttac
     5641 caggagatgc cgatatccgc ttcccgggta cgtcaactgc tggcgaaaaa cgatctcacg
     5701 gctatcgcgc cgctggtccc tgcagtcacg ctgcattatt tgcagaacct gcttgagcac
     5761 tcccgccagg acgcggcagc tcgtcaaaag acccccgcat gagaaacagg tgaaaaatga
     5821 aaataaacca gcccgccgtt gcaggcaaaa cagtatggcg attttgatcg ctatgtcctg
     5881 gccctctcct ggcaaaccgg attttgccag agtcaacacg atcgaaatcg taacgaacga
     5941 gatgaatgtc gcctgcaaac cgaaacgacc aacaaagctg attttctgac cgtacatggt
     6001 ctgtggccag gattgcctaa atcggttgct gcccgtggtg ttgatgaacg ccgctggatg
     6061 cgcttcggtt gcgctactcg cccaatcccg aatctaccag aagcgcacgc cagccggatg
     6121 tgttcatcgc cggaaaccgg gttatcactg gaaacggccg ctaaactaag tgaagtcatg
     6181 ccaggagctg gcggacgttc ctgcctagaa cgctacgaat atgccaaaca cggtgcctgc
     6241 tttggttttg atccggacgc atacttcggt acgatggtac gcctgaatca agaaattaaa
     6301 gagagcgaag ccggaaaatt ccttgcggat aattacggta aaacagtgag ccgccgtgac
     6361 tttgacgccg cctttgccaa aagctgggga aaagagaacg tgaaagcagt taagctaacg
     6421 tgccagggta accctgcgta tttgactgaa attcagatct cgatcaaagc tgacgccatc
     6481 aacgctccgc tttctgcaaa ctcattcttg ccacaaccac acccaggcaa ctgtggcaaa
     6541 acctttgtga ttgataaagt gggttattaa tcatctgatt tacaaaacac gctaattcgt
     6601 cttccataaa ccgtgacgat taagaattat acataatgcg ctgcattgag gaattccctg
     6661 aatgtgacgc aaatcacttc aaagagtgag caacagtcag tatcatcatg tgagttaaac
     6721 cctcgccgcc tgacggtgag ggttttcttt tgggtatgtt tatctgcgac actcgcagta
     6781 ccgacgacat ggagtaaaaa tgtccagacc aactatcatc attaacgacc tggatgccga
     6841 acgcatcgat attctgctgg agcaccccgc ctatgctggt ttgccaatcg ccgactcgtt
     6901 aaacgcagag ttggatcgcg cccaaatgtg ttcgccagaa gagatgccac acgacgtggt
     6961 gacaatgaac agccgggtta aattccgcaa tcttagcgat ggcgaagtgc gtgtgcgcac
     7021 gctggtgtat ccggcaaaaa tgaccgatag caatactcag ctttccgtta tggctccggt
     7081 aggtgccgca ctgctggggc tgcgcgttgg cgattccatt cactgggaac ttccgggcgg
     7141 cgttgcaacc caccttgaag tgctggaact cgaataccag ccagaagctg ctggcgacta
     7201 cctgctttaa tcccatctgc acaagcgata actcgctttg cccagaggat gcactcgcat
     7261 cctctttacg caaaatcagc aatttttcct ggttctcact gttcactttt gctgcaaaat
     7321 tattccgttt cgtttgtggg aataaccatg gcagaagcat tttacatcct gataggattt
     7381 ctgattatgg cggcgattat cgtcatggct gtcctttacc tcgaaaacca ctcctgacga
     7441 tcatattgtt ccccccggca tcgcattcac cagacctgaa actgccttct gcgccgcctc
     7501 tgcgctttgt gcaccgggta ccagaatcac cttacggcac tcctcttcac gtttttcgaa
     7561 aatctcatat ccccgggcgg cctcttcaaa cggcatatag tgggtaacaa tttcttctgg
     7621 tttcagtaat cctttctcaa ttaacggtaa taattctccc agccatgcgt gaacgtgggt
     7681 ctgtcccatt ttaaacgtca accctttatc aaaggcgtca ccaaacagga aaccgtgaat
     7741 aaatccagcg tagacgcccg gtacgctaac aatgccgcca cgcctgaccg ccgcaataca
     7801 ctggcgcaac gctttaccgc tgctgccctc cag
//
LOCUS       DAJTNA010000172         7824 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTNA010000172 DAJTNA010000000
VERSION     DAJTNA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.174"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            270..413
                     /locus_tag="OO161_003336"
     CDS             270..413
                     /locus_tag="OO161_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5464296.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            426..635
                     /gene="cspE"
                     /locus_tag="OO161_003337"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             426..635
                     /gene="cspE"
                     /locus_tag="OO161_003337"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5464297.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            801..1610
                     /gene="rlmA"
                     /locus_tag="OO161_003338"
     CDS             801..1610
                     /gene="rlmA"
                     /locus_tag="OO161_003338"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5464298.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTCGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="OO161_003339"
     CDS             complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="OO161_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5464299.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2601..3059)
                     /locus_tag="OO161_003340"
     CDS             complement(2601..3059)
                     /locus_tag="OO161_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5464300.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLEKIYKLIVSTQ"
     gene            complement(3114..3965)
                     /gene="manZ"
                     /locus_tag="OO161_003341"
     CDS             complement(3114..3965)
                     /gene="manZ"
                     /locus_tag="OO161_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5464301.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3978..4778)
                     /gene="manY"
                     /locus_tag="OO161_003342"
     CDS             complement(3978..4778)
                     /gene="manY"
                     /locus_tag="OO161_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5464302.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4841..5812)
                     /gene="manX"
                     /locus_tag="OO161_003343"
     CDS             complement(4841..5812)
                     /gene="manX"
                     /locus_tag="OO161_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5464303.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6275..>7824
                     /gene="yoaE"
                     /locus_tag="OO161_003344"
     CDS             6275..>7824
                     /gene="yoaE"
                     /locus_tag="OO161_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5464304.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDE"
BASE COUNT         1989 a         1915 c         1945 g         1975 t
ORIGIN      
        1 acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt gaactatgtt
       61 agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca atgatatatc
      121 ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg
      181 tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt gataacagat
      241 ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa gaagtcctga
      301 gtaaacacgt tgatgttgaa taccgcttct ctgccgagcc ttatattggt gcctcatgca
      361 gtaatgtgtc agttctatct atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt
      421 ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa ggttttggct
      481 tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct atccagggta
      541 atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag gacggccaga
      601 aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc tgaagtgtga
      661 atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact atgctttatc
      721 ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact tgcggccttt
      781 agtatcctgc cggagttgta atgtcttttt cctgtccact ttgccatcag cctctttcgc
      841 gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg aaagaagggt
      901 atgtcaatct gctgcccgtt cagcataaac ggtctcgtga tccgggcgac agcgcggaaa
      961 tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg cgtgatgcaa
     1021 ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg gatattggct
     1081 gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc accacgtgtg
     1141 gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg caggtcactt
     1201 tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac gccataatac
     1261 gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc ggcggctggg
     1321 tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg atttacaatg
     1381 aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta cagcagagtg
     1441 cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta ttgcagatga
     1501 cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac actggcagca aaagaagtgt
     1561 tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa ccgtggaagt
     1621 gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga atttccgctt
     1681 ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt gacataatca
     1741 aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg aaagcaagac
     1801 caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc agtagccaga
     1861 aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa ccctcaataa
     1921 tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc cattcaagga
     1981 caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg gtttcgacgg
     2041 caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg agggtggcac
     2101 ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga agaacagtag
     2161 cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca tcgcccccag
     2221 caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca ggcatagcga
     2281 ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc cacgttttgc
     2341 aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc ccaaatgagg
     2401 gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt atttcattgc
     2461 tttattgata acgaattcat ttagatttat atatcaatga aacgttaaaa taaataacaa
     2521 ataagggaag aggtaatata gcctaagcta tgtctgatta ttaaaaatac actaaacata
     2581 gctttggcta aattcataac ttattgagtt gaaacgataa gcttataaat tttttccaga
     2641 ttgtcgatat ttcgaacgcg gattaacagc cgacgctgtt ctaattgcat caccaacacg
     2701 ccatcttccg acaagttcat cgctttgatt cggctatatt caatccagac attggcgaag
     2761 aaaaaacctt tttgtttaaa gatgatcttc ggaacgcgga tccagaatat ataaaaaccc
     2821 atcagagcca gtgcgcttaa taaccatgtc gttattaacg caccatgatt cgtgacgttg
     2881 ttatagataa gaatgacaat caatccgacg aagataacgc tatcgatgcg accacgccgg
     2941 agcaaaggaa ttgccagcag ggtggggccg ttacggcggg gcatgatgaa ctgatcgtag
     3001 atcgcgaagg ccaggagtgc ggcgatgaaa agaatcagta ccaggtccgt gattgtcatt
     3061 aatcctccag ataaaaaaac ggggccaaaa ggccccggta gtgtacaaca gtcttacagt
     3121 cccagcaggc cgcaagcgta accagcgata ccgatgacga agaagccaac gatgatccac
     3181 agcgggttaa cttttttgcg cagtagccac atacaagcaa aggtcagcag cagtggtacc
     3241 aggcctggca ttaactggtc cagaatagtc tggacagtag taacgtgttc tttgcccgtc
     3301 tggtcagtaa tgcgagagac aaccagcggg atgttgacat gtgtccactt gttaaccaat
     3361 gcccccatga caaacaggcc gaggatagac gccccttccg tcagtttttg caggaagcca
     3421 ccacccatat ctttaacgat atcgatacct ttggagtaac catacgctac gccgtagtaa
     3481 cgggttgcca gacgcaccag gttaaacagg atgaagaaca gcagcggacc taacaggctg
     3541 ccgctcatcg cgataccggc acccagtgct gcaaataccg gacgtacggt tccccagaag
     3601 atcgggtcgc ctacaccagc cagcggcccc atcaaaccga ctttgatacc gttgatagca
     3661 ccgtcgtcga tctctgcgcc attagcacgc tgttcttcca gcgccagggt tacgccgaga
     3721 atcggcgcag ccacgaacgg ctgggtgtta aagaactcca ggtgacggcg aatagcttgt
     3781 ttacgagctt cgttgttctc agggtagagg cgacgaattg ccggtaccat agagaagcag
     3841 aaacccagtg cctgcatacg ttcgaagttc catgaaccct ggaagaggtt agaacgcagg
     3901 aagacgccac gaatatcact ttgagtgagt tttttctcgg tggtagtttg agttgtatca
     3961 accatttcgc tcacctgtta gtccagttcg ttatcgagat cgttgttacc agcagcctga
     4021 gcaggcgcac cggctacgcg gttgtatttc gggctaagtt ggatgtagag cactgccata
     4081 acagtaccga tcacacccag agcaaccagg ttaaagttgg tgaatgctgc ggttacgaag
     4141 ccgaggtaga agaacggcat caggtagcca gcacgcatca tgttgataac catcgcataa
     4201 ccaaccacca cgatcatgcc accggcgata ttcagaccat tggtcaccac ttccggaatc
     4261 gcattcagca tgttctgtac ttcgctggta ccaacagaca gcgcaacgat gacggccgga
     4321 atagccacac gcattgcttg caggaacaga gaagaaacgt ggatccagga aatcgctgtc
     4381 aggttgccgt tatcagcagc cttatccgca gcgtgctgga aagcaacggt aatagtacga
     4441 acgatgatgg tcagtacctg gcccgcagcg gccagaggga ttgccagtgc gatacctgca
     4501 ccaatgctct gatgacctgc gataaccaga atggtagaaa tgatagaagc cagagcggcg
     4561 tcaggcgcaa ctgcagcacc gatgttcatc cagcccagcg cgatcatttc cagcgtacca
     4621 ccgataataa taccggtttt catatcccca agaacgatac ccaccagggt acacgcgatt
     4681 agcggacggt gaaactgaaa ttcatcgagg attgatccca tacctgcgat acaggctacg
     4741 ataaatacca gcacaatttg aagagtggta atctccattg tacttctcct gtttacgtaa
     4801 gacttaagtg tgaaaactga gtgataatca acacaatacg ttacttatcg attttgctga
     4861 tcagatccat cattttcagt ttcggatcgg tggaaacctt acggacttcc agctcaatac
     4921 cgcgcgcatt cagtttcttg aacgcctcga tatctttttc atcaaccgaa accgcgttat
     4981 tcacctgggt tttaccttga cggaatgcca taccaccgac gttaacagag gtgattttca
     5041 cgccgccttc aacgagacgc tctacatctg ttgggttggt aaataacagc attacgcgtt
     5101 cgccagcata tttcgggttg ttgtagacgc gaatcatttt ggcaacatca actacgtgtg
     5161 ctgttacgcc cggaggtgca acctgggtga gcagtgtctt acgaacggta tccgcagcca
     5221 cttcatcact aacaacaata atacgggaga cattggtttc tttggtccag cgggtggcga
     5281 cctgaccgtg aatcagacgg tcgtcgatac gcgcaaggcc aataaccatg tagtcgtttg
     5341 gccccattgg ttttgccgga gttggagccg cttttggtgc tgcggcagcg ggtgctggcg
     5401 cggctttttc aaccggtttg gctttcagtg ctttcacgcc ttcacggcct gtttctactg
     5461 ccagcgcaac cagttcatca aagcttgggt catcatcacg agccattaac gtttccacga
     5521 gcattggaat gttaacgcct gcaatgactt cataatgctc tttgtcgacg acaatgcggc
     5581 tggcagcatt gaacgggctg cctccccatg tatcaacgag aaacagcacg cctttagtgg
     5641 tgtcgagttt tgccaactga gcgttgtact tttcaatcag cgtttcggca ttttcacctg
     5701 gaacgaaatc gatccagccg acgttttcct gctcgcctaa cagcatttct gccgttttaa
     5761 gcaactgctc tgcagcccaa ccatgtgtgc ctataacaat agcaatggtc acttgctacc
     5821 tcctttatta tcgttaacac ctcaacgtgc cagatgtatt tttgaatcgt cgtccacaat
     5881 cgaatcgatt cagataaggg ctacaaccaa aaacccctgc gtttcgcgat ttattttaga
     5941 tatcgaaaaa attattttat gtgatgaaga tccgtaattt aactttcgat tagcagaaat
     6001 ttcgaaaggt aaaatatcct tgtcacattc gtttgcaaag gaaggtaaat ctttgccaaa
     6061 tcagaggcgt ctctgatatg tttaactccc gtttaacaac catggagtat agggcagtag
     6121 cccgcagtat ggatcgtcac cgacgtcatt tcagcatcag gccttttaac gcctgccttt
     6181 ctggcactct atgccgcacc tttcgtttgc attttgtcgt tacgcctgca ttatttctgg
     6241 cgtcgaatag ctattcctta agcaggagct tgtcatggaa ttcttaatgg acccctcaat
     6301 ttgggcgggg ctactcacgc ttgttgttct cgaaattgtg ctgggtatcg ataacctggt
     6361 cttcatcgcc attcttgctg acaaactgcc gccaaaacaa cgcgataaag cgcgtttgct
     6421 ggggttatca ctggcgctga ttatgcgtct ggggctgctg tcgctgattt catggatggt
     6481 cacgctgacc aaaccgctat ttaccgtcat ggatttctcc ttctccggac gcgacctgat
     6541 tatgttgttc ggggggatat tcttgctgtt caaagcaaca accgaactgc atgaacggct
     6601 ggaaaaccgc gatcatgatt ccggccacgg taaaggctac gccagtttct gggtggtcgt
     6661 cacacagatt gtcatccttg acgccgtctt ctcgttggat gcggtaatta ctgcagtagg
     6721 gatggttaac catctgccgg tgatgatggc ggcggtagtg attgcgatgg cggttatgtt
     6781 gctggcatcc aaaccgctga cgcgattcgt taaccagcac cccacggtgg tggtgctctg
     6841 tctgagcttc ctgttaatga ttggtctgag tctggtggca gaaggtttcg gtttccacat
     6901 tccgaaaggt tacctgtatg ccgcgattgg cttctcgatc atcatcgaag tgtttaacca
     6961 gattgcgcgt cgcaacttta ttcgccacca gtcgactttg ccgctgcgag cgcgtactgc
     7021 cgatgcaatc ctgcgtttga tgggcgggaa acgtcaggcc aatgttcagc acgatgccga
     7081 taacccgatg ccgatgccga tcccggaagg tgcatttgcc gaagaagaac gttacatgat
     7141 taacggcgta ctgacgctgg cgtcgcgttc tctgcgcggg atcatgacgc cgcgcggtga
     7201 aataagctgg gttgacgcta atctcggggt cgatgaaatc cgcgagcaac tgctctcttc
     7261 accgcacagt ctgttcccgg tatgtcgcgg tgaactggat gaaatcatcg gtattgtacg
     7321 tgctaaagaa ctgctggtgg cgctggaaga gggcgttgat gtggcggcga ttgcttcggc
     7381 gtctccggcg attatcgtcc cggaaaccct cgatccgatc aacctgttgg gcgtgctgcg
     7441 tcgtgctcgc gggagctttg ttatcgtgac caacgagttt ggtgtggtac aaggtctggt
     7501 cacgccgctg gatgtgctgg aagccattgc gggtgaattc ccggacgctg acgaaacgcc
     7561 ggaaatcatt actgatggtg acggctggct ggtaaaaggc ggtacagatt tgcatgcctt
     7621 gcagcaggcg cttgatgttg agcaccttgc cgatgacgat gatatcgcga cggtcgcggg
     7681 cctcgtgatc tcggcaaatg gtcacattcc ccgtgtgggc gatgtgattg atgtagggcc
     7741 actgcatatc accatcattg aagccaatga ttatcgtgtt gatctggttc gcattgttaa
     7801 agagcaaccg gcgcacgatg aaga
//
LOCUS       DAJTNA010000173         7821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTNA010000173 DAJTNA010000000
VERSION     DAJTNA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.175"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(105..4007)
                     /gene="hrpA"
                     /locus_tag="OO161_003345"
     CDS             complement(105..4007)
                     /gene="hrpA"
                     /locus_tag="OO161_003345"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5464305.1"
                     /translation="MTEQQKWTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAFDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4208..4813
                     /gene="azoR"
                     /locus_tag="OO161_003346"
     CDS             4208..4813
                     /gene="azoR"
                     /locus_tag="OO161_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5464306.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIACGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4864..6180)
                     /locus_tag="OO161_003347"
     CDS             complement(4864..6180)
                     /locus_tag="OO161_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR5464307.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHLRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(6170..7821)
                     /locus_tag="OO161_003348"
                     /pseudo
     CDS             complement(6170..7821)
                     /locus_tag="OO161_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT         1919 a         2171 c         1869 g         1862 t
ORIGIN      
        1 cattacctga attcctgaat ttttaccctt accgttttag aattaataaa aaacccggcg
       61 ctaaatgctt accgggtttt agatttatta ggcaaatagc agggttaacc gctaatctgc
      121 tccatcgcct gcaaaatacg cttatctgaa atcggataag gcgtaccaag ttgttgagcg
      181 aagtaactaa cgcgcagctc ttctatcatc caacggatct ctttcacgtc ttcatcctca
      241 cgacgtgcag gcggcagttt gttgatccat tgctgccacg cctgctggac gttttcgact
      301 ttcagcatct gtgcacgatc gcgatgtgga tcaaacgcca gtttttccag tcgtttttca
      361 atcgcctgca aatatcgcag cgtgtcgccc agccgtttga agccgttacc agtgacaaaa
      421 ccgcgatata ccaacccgcc catctgcgct ttaatgtcag aaagccccag tgccatggtc
      481 atatccaccc gccctttcag acgtttgttg atattgaaca ctgccgtaag aatttgctcg
      541 acctgcttcg caatatctac caccgtgtcg ttcagttcgg cacgcacttt ctcatgcagc
      601 gcagcaaagc cttcttccgt ccagaccggg ccaccattgg cgtcgatcag ttgatccaca
      661 ccgcaggaga tacagtcgtc gatcagctcc agcactttgc catacgggtt gaagtacagt
      721 cccagcttgg ctttgttcgg taacttttca tgtaaatact tgatcggcga gggaatattt
      781 agcagcagta gacggcgaag accattccac attgcctgtt tttgctccag cgggttatca
      841 aacagtttga tcgccacgct gtcgcgttca tccaccagcg ccggccacgc cttcacttta
      901 tagttgccac gcttctgttc gtaactttcc ggcagctgac caaaactcca gatatgtaag
      961 ccgctctgct cgataccgtc atccgccacc gcggacagcg tttcctgcac tttgcctttc
     1021 agcgcatctt tcagatcttg tagcgagcgc ccttctttta gcttcttgtt tttgtcatcc
     1081 accacgcgga aggtgatttt caggtgatcg ggcacctgat cccagtgcca gtcttcgcgg
     1141 tcaacggtaa cgccggtcat ccgccgtaac tcgcgctcaa ggctgtcgag caacggtaac
     1201 tccagcggtg tgacgcggcc taaaaacgct tcggcgtagt ttggtgcagg cacaaaatta
     1261 cggcgtaccg gtttcggcaa cgatttaatc agagcaatca ccaattcgcg gcgcagaccg
     1321 gggatctgcc attcaaaccc gctttcctca acctggttaa gtaacggcag cggaatatgt
     1381 acggtcacac cgtccgcgtc tgcgcctggc tcaaactgat agctcaaacg cagcttgaga
     1441 ttgccctgat gccagaagtt cgggtaatcc agcttgctga ttttttccgc cccttctttg
     1501 atcaacatgc tcttttcaaa gttgagcaaa tcaggcgttt cgcggctgac ttttttccac
     1561 cagctatcga agtgacgagc ggagattaca tcgtggctga tgcgctggtc gtagaactca
     1621 aacagcgttt cgtcatcaac cagaatatcg cggcgacgtg atttgtgttc cagctcttcc
     1681 acttcggccc gcagtttcag gttttcacgg aagaatgcgt gacgcgtctg ccagtcacct
     1741 tccaccagcg catggcgaat aaagagttca cgacataacg ccggatcgat ctggctgtag
     1801 ttgaccttgc gcgcggcaac aatcggcaaa ccataaacag tgactttttc cgttgccatc
     1861 accgcgccct gcgcccgttc ccagtgcggt tcgctgtagg tacgtttaat caaatgctga
     1921 gcgacaggct ccacccattc cgggtcgata cgcgcagcaa tgcgccccca caggcggctg
     1981 gtttctacca gttccgccac cattacccat ttcggcggtt ttttgaataa accagaaccg
     2041 gggaagatgg agaaacgcgc gttacgtgcg ccggtatatt cttgtttatc ggcatctttc
     2101 atgccgatat gggaaagtaa acccgtcagc aacgcaatgt gaatttcgcg atactccgcc
     2161 ggttcgctgt taaccggaat gccaagttct ttcaccacct gacgcaactg ggtgtagata
     2221 tcctgccatt cgcgcacgcg cagatagttg agataatcgg tacgacacag gcgacggaag
     2281 gcgtttgaag aaagcgcctt ttgctgctcg ccaagataat tccacagatt tacaaacgcg
     2341 aggaagtcgg attctttgtc gtggaagcga cgatgttttt cgtccgatgc ctgctgtttg
     2401 tccatcggcc gctcacgcgg gtcctgaatg gagagcgcgg acgtgataat catcgcctca
     2461 cgcacgcagc catgtttctg cgcttccagc accatacgcg ccagacgagg atcgaccggc
     2521 agttgcgaga gctggcgacc gagcggcgtc agtttatagg cgctggcctg ttcatccgtg
     2581 gtgatcgcgc ccagttcttc gagcagacgc acgccatcct ggatattgcg tttatccggt
     2641 gcttcgacaa acgggaacgc agcgatatcg cccagcccca gcgcggtcat ctgcaaaata
     2701 accgaggcca ggttggtacg cagaatctcc ggatcggtaa actccgggcg cgagaggaaa
     2761 tcgtcttcgg aatagagacg aatacagatc ccttcggaca cacgaccaca acggccttta
     2821 cgctgattgg cagacgcctg tgaaatgggt tcaatcggca aacgctgcac tttggtgcga
     2881 tagctgtagc ggctgatacg cgccgtaccg gggtcgataa cgtatttaat ccccggtaca
     2941 gtcagcgagg tttccgcgac gttggtcgcc agcacaatgc gtcgcccgct gtgcgactgg
     3001 aacacgcggt tctgttcgct attcgaaagc cgcgcataaa gcggcaagat ttcggtatgg
     3061 cgtaagttca gcttgttcag cgcatcggcg gtatcgcgaa tttcccgctc gccgctcata
     3121 aagatcagaa tgtcgccagg gctttcctgg ctcagttcgt ctacggcgtc aaaaatcgcc
     3181 tgcaactgat cgcgctcggt gtcatcggct tcttcaacaa tcgggcgata gcgcacttcc
     3241 accggatagg tccgaccgga gacttcgata atcggcgcat tattaaagtg gcgagaaaag
     3301 cgttccgggt cgatagtcgc ggaagtgata atgattttta ggtcaggacg ccgcggcagc
     3361 aactctttca aatagccgag cagaaaatcg atattcaggc tgcgttcgtg tgcttcgtca
     3421 ataatgatgg tgtcgtactg catcagcagg cggtcttgct ggatctccgc cagcaggata
     3481 ccgtcggtca tcagcttgac catcgtgtta tcacttacgt gatcgctaaa acgcactttg
     3541 taaccgatgc aaccgcctgg ctccgttttc agctcttccg caatacggtt cgccaccgtt
     3601 cttgccgcca gacgacgcgg ctgggtatgg ccgatcagtc ctttaatccc gcgccccagc
     3661 tccatacaga ttttcggtag ctgggtcgtt ttaccggaac cggtttcccc ggcgacgatc
     3721 accacctggt gatcacgaat cgcttcgaga atatcctgtt ttttctgact aactggcaaa
     3781 ttgtccggat aagtgatttc cggtcgtgcc gcttcacgca gcaggacttt ccctgccgcc
     3841 tggtcaatct ctttcgccat ctcctggaaa atggcctgtt gtgcatcagg atttttaacc
     3901 ttcttaacac catgcagacg gcgagaaaaa cgcagtctgt cgcgcagcat caacgaatcc
     3961 agccgctgct gcaaagcagt aaaggtccat ttttgttgtt ctgtcataac gttagtgggc
     4021 agtagtctgc ctgattaatt ttcttttaaa tgatagatat agagtaccac atcgcggctt
     4081 tctatgcgtt gttcaataaa tccgaacata ggcttcgatt tattgcgcta tctctgtggc
     4141 aggattgtga ataaagtgtc agcaagcaac ggggcatcgc caattcaaac atctataagg
     4201 aaacaccatg agcaaggtat tagttcttaa atccagcatc ctggcagggt actctcagtc
     4261 taatcagttg tccgattatt ttgttgaaca atggcgcgaa aagcactccg ctgatgaaat
     4321 caccgttcgc gacctggctg caaatccgat tccggtactg gatggcgaac tggttggcgc
     4381 tctgcgtccg agcgatgcgc cgctgactcc gcgtcagcag gaagctctgg cgctttccga
     4441 tgagctgatt gccgagctga aagcccacga cgttatcgtt attgcggcac cgatgtataa
     4501 cttcaacatc tcaactcagt tgaaaaacta ttttgacctg gttgcacgcg caggcgttac
     4561 tttccgctat accgagaacg gtccggaagg tctggtaacg ggtaaaaaag ccatcgttat
     4621 taccagccgc ggtgggatcc acaaagatgg accaacggac ctggtgacgc cgtatctgtc
     4681 cacgttcctc ggctttatcg gcattaccga tgtgaaattt gtcttcgccg aagggatcgc
     4741 atgcggtccg gaaatggcag cgaaagcgca gtctgacgcg aaagcggcca tcgacagcat
     4801 tgttgctgca taaccggaca cgatcttgat gtggactgta gttcagtcca catctcaatc
     4861 cacttacctg ttttcccata atttcagcat cgctttgtga tcttccttaa gcacaatatg
     4921 cgaacgtctg gctcgaatat aactaatcgc ttcatcaacg gttttacagt gtccgtaaca
     4981 taacaaccat gccgccacta ccagcgcact gcgcgataat cccaacgcgc aatggaccag
     5041 aacgctgcct tgctcttcgc gtaatgtttc cagcatcgcc acggcctgtc ggagctcccc
     5101 ctcttccgga accaccagat ccagcatcgg tacacagaaa tagagtcgat cttttgtcgc
     5161 tcgtccccga gggaattcaa aggtgacgtc cagaaccgca ttctgtgccg gaatatgtcg
     5221 tggaaacgcc cctaaataaa caccagcagt aattttgctc accggctcca ggcgacgaca
     5281 aaaccaacgc atagacagcc acatcccgat gcgccagggc aatgtcagcc agtaaacggc
     5341 gggcgggagt ttcccctgac tatctttccc tgttgttatc gcgccaagcc caccgtaccc
     5401 acggccaata atgagtagcg ataatactgg ccaacataac cagactgacc accataactg
     5461 aatcatcacc attagctcca tcaacacaat gcacgagccc gcgcctacga catatggcag
     5521 tgctattttg attcgacgtt gatcaggttt ctgataattc caacgacgat cgacgggcac
     5581 catccagtca atcaacatac ctaccgccag ccctgtgatg acatcaataa aatgatgctg
     5641 ccaggtcgtc agagtagaaa tagcgatgag taaaaaccat ccgccacaga ctttacgcca
     5701 cctctcagcc agatgctgac gcaagtgacg ccaaagtagc cagcagagaa taatatgcag
     5761 cgaaggagac tggttataag gcagatcaaa cagttcaagt tgcgaaaata gccatcccgt
     5821 caccccactc acttcaggac ggataaaact aaacttcagc ggatagagca aaaaaccgca
     5881 gcaggccatt accgttgcta gaataagccg gtggacaagt cggcgctgtt cgaatgtcgt
     5941 gctacaaacg aacagcgaaa atccatataa aagatccaga ctccagtaag gaacaatagt
     6001 ccagggaaga aaagggatcg ccgtttccca accgaatacc tgactgggga tatcatggct
     6061 gttaaggtcc tgaaccgcgg tgaactgatt aagagatcca taggtgaaga aaaaaaacgg
     6121 ggccagcaac aataaccagc cagcgccttg tagcaatacg ttccgacgtt cagttatcac
     6181 gacgcaccgc catcgaaacc gtaaaaatac cccactcatc aatccgttgt gtgcatttat
     6241 caaatccggc atcacgcacg agtgaatcca tctccccttg cgaacgcacg cgcattaccc
     6301 acggtttacc atctttatga ctggttaaca ccccggcaat catctccagt tgagggtgcc
     6361 acggctgccc ggtgtagatg agaatgccga ccggttcgat ggcattggca agacctgcga
     6421 gtgagttttt tacctgctca ttttcgggaa aaagctcata cagtccagag acaattgcca
     6481 gcgtaggccg cggtgttaac gcgctgagtt cctccgggtt aaacgcatcg ccctgttcaa
     6541 aacgcacccg cccagacatt ccccgttggg caatcatctc ttgcccctgt gcaacattta
     6601 actcgctgta atcacgtaac aaaatatcgc ttacggcagg ctcgtttgcc agcgcatcca
     6661 gtacatagcg cccatgccct gcggcaatgt caaccacgcg gacggctaaa cctttggcgt
     6721 ggagatcggc aacggcttgt ttaatcagta tttgtaaatg ggttttgcgc tggcgaatac
     6781 cgcgccagcc aacactgttc aggtagattt tgtcgattaa gcgcccgaat gcgttactac
     6841 cttgcggttg attttgatag acataatcca gcgaactgcc tgaatcaaag ccggtgctca
     6901 ttccgagatg caggcccgca gagtgcgtcc cgaacaattt catcgccttt cgcataaagc
     6961 gatacgctaa atcaaccggc gataatggca cgggtccacc agaaagtagc cgccattgat
     7021 ccgctgatgg tcctgtgcag tcttcatgct gataatcaaa tttttgcgat ttgttagcat
     7081 ataaacgact aataaagctt tgcatttttt caaatgccag cgccctgttc tcttcaccct
     7141 acgtgtcgtg ataaaagcct ggcagcagat gcagctcttt cagagggcta cgtaatctct
     7201 gataaaaatc aatctgcggt tggcgatgca ccacatagtc atcgcctgat atcagaagtt
     7261 gcgtggggag cgtaatcgcc gccgcatctc taataatacg ttcagacgtt ttatagagat
     7321 cgagcaagat attgacggca atcgcccgtg tgatcagcgg atcattattg aaactcgcca
     7381 cccgttgccg atcgtgggtc aaatagcgtc ctttcacata ggaattaata aaaaacagac
     7441 cacgcagacg atgccataac gccagcgcag gacgtgccag cggcacatac aatttaacct
     7501 taaaggccgg agaagccagc accagcccgc gaattgcagg cgcataatca tgaacctatg
     7561 tggcaaccag cactgcgccg acgctttgcg cgatcacaac cacctcttcc agtccaacct
     7621 agctgtcgct ggcagcaaaa cggacaaatt catcgacatc ctgcactgaa cgcgcaagat
     7681 atgggctata accacgcggc cccgaagttt ttccatgccc ccgggcatcc catgcataaa
     7741 aagcagtatc tggcatcgcc agttcatcaa cgatatgttg tagacgacca gaatgttcat
     7801 ggccacgatg aaataagaca a
//
LOCUS       DAJTNA010000174         7799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTNA010000174 DAJTNA010000000
VERSION     DAJTNA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.176"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            567..3980
                     /locus_tag="OO161_003349"
     CDS             567..3980
                     /locus_tag="OO161_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5464308.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRD
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQITRLQKIVEELGVVTDAATV"
     gene            4044..5678
                     /locus_tag="OO161_003350"
     CDS             4044..5678
                     /locus_tag="OO161_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5464309.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLEAVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5687..6757
                     /locus_tag="OO161_003351"
     CDS             5687..6757
                     /locus_tag="OO161_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5464310.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLMGF"
     gene            complement(6882..7097)
                     /locus_tag="OO161_003352"
     CDS             complement(6882..7097)
                     /locus_tag="OO161_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464311.1"
                     /translation="MGVNELYLAYDFTFFLRMMPYMEPGLIKRDMGELLKILKKHIDE
                     AMNTQVLSSNFCKLVCIYDYIKNSHRY"
     gene            complement(7364..7711)
                     /locus_tag="OO161_003353"
     CDS             complement(7364..7711)
                     /locus_tag="OO161_003353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464312.1"
                     /translation="MFVEWKPELEEADISDSVIHKKIPVKYVQAPSYKEIFEVLSETK
                     KRIPAHLFRMIKDELYELVLTDDPKGKLYVRDSEKIEEGVSTTEFVVGYGAISMVKNR
                     NRWQLKDWLDWSV"
BASE COUNT         2273 a         1770 c         1815 g         1941 t
ORIGIN      
        1 cagtcctgtt ctcccaaata tcatggtatt gatacgggag attatgccgt acaggtacga
       61 cacgttatgt cgtgccaaag gggagtggat agaaaaaatg gcgaagatgc gaaaagttga
      121 ttaatgcatt gaaaaaaatg aattaataat gaacttgggt aaaagcgcca tcaaaatacg
      181 acacgacctg tcgtattttg tgttagccgg gaaaagatat ctgctactta ctaaaatgat
      241 ttagtgctga aaatatttat aagccaataa atagacaggt attacggagt aacgcccagg
      301 ctattttaac gagcctgctg ttttattggc tcaaaaaaca tatcccatag atgatcagcc
      361 atacccgcgt tagtgagcaa ggtatccctt gagtagccag ggctgaagac gcccattgat
      421 gggcgaaaac caaggttctg gccgttaaag cggctatgtc gttaaacaca tcaccaccat
      481 atgccatccg gctcacttcg tcggccaaat tagtgtgata ttctttccag aatttctgtt
      541 gtatccacaa cataaggcga tgattgatgg agcagtctct caattttgaa atgttgcgca
      601 gccagtggcc ggaactggca gaactcgcat gtatggcaga gcgctatgtt cactccgatc
      661 cggaaagctg cctggttaag ctgcgcaact acaccgaatt gatggtgcgc tggttgtatc
      721 gtcaggagcg gttgccggaa ggtattaagg ctaatcttta cgatttaatg aacgctgatg
      781 tctttaccag catgatgccg gaagccatca tcatgaaaat ggatgcgctg cgtatccatg
      841 gcaaccgtgc cgcgcacggc ggacgtatca aagctaaaga tacttactgg ctgctcaaag
      901 aagcgtattt gttgggaatt tggctgtatg ttcgctacgc ccacggtaat gttgatgact
      961 gccccaaatt tacactccct ccattaacac aatcttcagg tcgtgcagat gaaaaacgtc
     1021 tggaagatgc aatcaaggct caggatgaaa gccgtgagcg cgaactggcg ctacaacgcg
     1081 cactacagca agaacaggaa aaggccgaac acctcactca acgcctgaat gaagccagag
     1141 cgcgtaacca gcatgttgcc gatattctct ctattgatga agcggaaacc cgccgtcgcc
     1201 tgattgactc tcgcttactt gcagccgact ggaatgtagg ggaagaactt aaaaacacgg
     1261 atcaagtcac acaagaacat ccagtcaaag agcaacccac cacctctggt gacggttatg
     1321 cagactatgt cttgtgggat gaggcacaca aaccgttggc ggtggtggaa gcaaaaaaaa
     1381 ccagcgtcaa tgccgagcaa ggacgaattc aggcccggct gtatgcagac tggctggaaa
     1441 aagaatacgg tcagcgtccg gtcatcttct acaccaacgg ctatgatatc tggctgtggg
     1501 atgaccataa aactcatggt tatcccccgc gtcgggtgtt tggcttctac agcaaggaaa
     1561 gcctgcaata tcttattcag cagcgtgaaa cccgtctgcc gctgaacaga gtgccacacg
     1621 taaaagataa cgaaggtaag gccgttgccg gacgtttgta ccagctcgaa accattgccc
     1681 gcgtcagtga acggtttacc aacaagtacc gacaatcgtt aattgttcag gctacgggta
     1741 caggcaaaac ccgcgtggca attgcactaa gcaaactgat gattgatgcc cgctgggtaa
     1801 aacgcgtcct gtttctttgc gaccgtaaag aactgcgtaa acaggcggca aatgccttta
     1861 atcaattcac caatgagccg ctgtatgtgg tcgggaaatc gaaaaaagca gacaggcaga
     1921 atgccagaat ctacatcgct acctatcccg gtatgatgaa aatcatggac catttcgatg
     1981 tcggttattt cgatttgatc atcgccgatg aatcccaccg ctctatctac aacgtctacg
     2041 gcgatctgtt taagtatttt gatgccctcc agattggcct gacggccacg ccaattgaca
     2101 tggtgagtaa aaccactttc ggcctgtttg gttgtgaagg acgtattcct accgccaact
     2161 acagcctgga agatgccatc gccgataaca atctggttcc ctatgaggtt gtcacacaca
     2221 caacagaatt cctgcgcgaa ggtatcaaac gggagaaatt aagcgacgca caaatccgcg
     2281 agctggaaga acaaggcata gatcccaata cgctggagtt tgatggcaag gcgctggatg
     2341 aggcgatcta caacaaagac accaaccgct atatcctgcg taacctgatg gaaaatggcc
     2401 tgaaagatcg ggacggccag ttgcctggta aaaccatcat tttcgcccgt aaccacaaac
     2461 acgcgctgct gttgaatgaa ttgttcgacg atatgtaccc acagtttgcc gggcgcttct
     2521 gccaggtcat cgacaactac gatccccgcg ccgagcaact gattgatgat tttaaagggc
     2581 tggatgaaag caccaacaaa gagctgacca tcgctatctc cgttgacatg ctcgataccg
     2641 gtattgatgt cccggaaatt gtgaatctgg tctttgccaa accggtcaaa tcaaaagtga
     2701 agttctggca gatgattggc cgtggtacgc gcctctgtcc ggggttgtac ggttacgacg
     2761 ataacggcaa accgctggat aaacagaaat tccgcatttt cgatcactgg ggcaattttg
     2821 agtatcacga gctgcatacc gaagaggcgg aagtcacagc gacaaaatcg cttgcgcaaa
     2881 aacgcttcga agcgtgggtt atgctggggg ccgctgcgca gcgtaaattc gacaaacagg
     2941 cggtggattt agttgctcac cagcttcgcg agcaaatcaa cgcactggat gaaaagtcga
     3001 ttgccgtaca ggaaaaatgg cagcagaaag cgcagtacag tgatgaaaag gtgctgcgcc
     3061 agctttctcc gaaaacacag caggatctgc tatctgttct ggccccgttg atgcagtggc
     3121 tggacgtgcg cgggcaaagc gatgccatac gctttgatat ggatattctg gcggcgcaaa
     3181 ctgcccgtta taccaacccg gaagagctgg atgttctctg gccgatcatc gtcgaaaaag
     3241 tggagcgcct gccgccgcat ttggcgcagg tgcagcaaca gggacaacga atcaatcagc
     3301 ttcgtgattt aagctggtgg aagcaggcca gcctggaaga gctggaggat attcgcattc
     3361 atttgcgcgg tatcatgcac ctgatggaaa aagacgcgac gcctaaattt ggttcgatac
     3421 aggtggatat taccgaagat gcgaacctga tccagacgga gacccgcaaa actaatatcc
     3481 gctcgattga tttcaaactc tatcgccagc aggttcaggg ggcgctggag ccgctgttcc
     3541 agcaaaaccc ggttctgaag aaaatccgca acggcgagcc agtcacgcaa aacgagctgg
     3601 atgagctggc gaagctggtg ctgatccaga accccaacgt tgatattcgt gcgttgaaag
     3661 agttttatcc gcaggcaacc gccagcctgg ataaactttt gcgtaccatc atcgggatgg
     3721 acagcgacgc ggtggaagtg cgttttgccc agttcgccgc tgataacagc ctgaccagcc
     3781 aacaactgcg cttcctgtca ttgctgaaaa accacattcg cgattacggc accattgaaa
     3841 tgcggcagct ctttgaacag ccttttacgc atatccacaa cgaaggcgtt accggtgtgt
     3901 tccctgatat agcgcaaatc acccgcctgc aaaagatagt cgaagagctg ggtgttgtga
     3961 ccgacgcagc gacggtataa tacacgcatg acaagccccg gcagcggggc ttttgtattt
     4021 aaaacaggat ataaacgcta acaatgatta ctggcgactt aaaaagtaaa atcgacggac
     4081 tatgggaaga tttctgggtg ggtggtatca ccaacccgct gaccgtaatc gaacagatca
     4141 cctatctgat gtattcccgg atgctggata cccaggaaca acgcgacgaa aagcgtaaac
     4201 aaatcgcggg tattgatttt aaaccgcgtt ttgcgccaga acagcaggag ttccgtttca
     4261 gtcactatag caaccttggc tcggatgaga tgatggaagt ggtgcgcgat ggcgtattcc
     4321 agcatttccg tcagctcggc caggccgatg cttcgaaggt gacgctgctg ggcaacttta
     4381 tgaaagatgc tcgtctggag attgttaagc cgtcattgct gaccaaagcg gttgaggtaa
     4441 tcaaaaacct gccattggat cgcggcgaca ccaaaggcga cctttacgaa tacttgttaa
     4501 gcaagctgac aactgccgga atcaacggac agttccgcac accgcgccac attatccgca
     4561 cgatggttga aatgatggag ccgaacccgg cccgaggcga gacgatttgc gatcccgcct
     4621 gtggcaccgg tggttttctg gcaaccagct atgaatatct gctggagaag tacagctcgc
     4681 tggaatccat tcatactgag attggcacca acgaacgtgg cgagctggaa gagcaaaaaa
     4741 tctttaccgg cgatctgctg acaccgtggc gtaaccatgt ggataacaac atgttccacg
     4801 gttacgactt tgacaccaca atgctacgta tcgccgccat gaacctgatt atgcacggcg
     4861 tggatgcgcc tgatatccac tatcaggaca caatgagcca gagtttcagc acaaacttcc
     4921 cgcaggccag taaaaacgcc ttcaacctga ttctggcgaa cccgccgttt accggttcac
     4981 tggacgaaga agatatcgac tccacgctgt cggcaatggt gaaaaccaaa aaaaccgaac
     5041 tactgttcct ggcgcgtatt ctgcaaatgc tgaaagtggg cgggcgcagt gccactatcg
     5101 tgccgcaggg cgtgctgttt ggctctagca aggcgcacca gtcacttcgc aaaacgctgg
     5161 tggaagataa ccaactggaa gcggtgatca atctgccttc tggtgtattt aaaccttacg
     5221 ctggcgtggc gacggcgatc ctgatcttta ccaaaggcgg tcaaacggat gaggtctggt
     5281 tctacgatct acaaaatgac ggctacagcc tggatgataa gcgcaacccg ataaaagaca
     5341 acgatctgcc gcatctgctg gcaagctgga agcattaccg tactttacgc gggctaccgg
     5401 ttgataactt tatgggtaag aagttagcct cgttgcttaa acagcagtac ccggaaggga
     5461 ttaatgctgg cgttgatttt aaagatcgca cgcaggcggc gtttgttgta ccgaaagcgg
     5521 atattgctgc gcagaaatac gatctatcca tcaaccgtta taaagaagtc gtgtatcagg
     5581 cggaggaata tgaagatccg aaggtgatat tgaagcggtt aaaggatctg gaaaaagaga
     5641 ttctggcgga tttggatgag ctggagggga tgctgtgagt tcggcgatgg tgaagttagg
     5701 ggatgttatt aatgttcact acggaaaagc attaaaagct gatcagcgtg tatcgaatgg
     5761 aagtgtccat gtttttggta gttcaggaat tgtcggcaac cacgataaaa cgctttgcag
     5821 ctatcccacg ataattattg gccgcaaagg cagcgttggt gctattacct gggctccaag
     5881 cggaggctgg atcattgata ctgcttatta cgttgagatc aaagataata ataagttaga
     5941 tctccgatac ttgttttata ttctgagcgg aattgatctc actaagaaaa cgattaccac
     6001 aagtattcct ggattaaata gagacgatct ctatgataca tttattaaat taccaccttt
     6061 tgaagagcag aaacgaattg tggatcttct cgataaagct gagggtatac gccaaaaacg
     6121 tgagcaatcc atcaaactgg ctgatgattt tttgcgtgct acgtttgcta caatgtatgg
     6181 gaatcctatt acaaacccca aaaaatggcc tgtccatctt atgggagaaa ttattgaatt
     6241 taaaggtgga aatcaaccac ctaaaagtga ttttattttt gaacctaaac agggttatat
     6301 tagattagta caaattagag attttaaatc cgataaatat gcgacgtaca ttccgcaaga
     6361 aaaagcaaaa cgtattttcg aagttgatga tgttatgatt gctcgttatg ggccacctgt
     6421 gtttcaaata cttagaggtt tatccggcag ctacaatgta gctttaatga aagcttcacc
     6481 caaagaaaat attaggaagg ggtttatatt ctatcttcta caacttccgg aatatcatga
     6541 tgttgttgta aaaaactctg aaagaacagc aggacaaacc ggtgtaaatc ttgaactatt
     6601 aaacaaattc aatgttcccc tcccacctat atactaccaa gatgagatat tagatagatt
     6661 agctagaatt gaaaaattta aagaaaaaat agaaatatca ttaaatcatt tagaaataca
     6721 atttttatca ctacagaaga gattaatggg tttttaaatc cttagtatcg aaataaacat
     6781 taatttatta tattaaatta agatatttta ggaaattaag gcgtcactct taggtaacgc
     6841 ctttttttac tatctcgccc cctaagaagg ttatatctta cttaatacct gtgactattt
     6901 ttgatataat cataaataca cactaatttg caaaaattac ttgacaatac ctgagtattc
     6961 atagcttcat ctatatgttt tttcaatatt ttgagaagct ctcccatatc gcgcttgatt
     7021 aatccaggct ccatataagg catcattctt aaaaagaaag tgaaatcata agcaagatat
     7081 aactcattga cccccacctt gacctcagga acggtttcag agcgtcgttt atcccatcct
     7141 tgagactgaa aactaatagg agtgatattt aatcgtgata aaacgccacc tgagagttct
     7201 gtttcattaa ttatactccc atcaggatta attaatttag cgtgatttaa gaagtgaaaa
     7261 ataggaatgc gcgcattgcc tttgcatata tttggcaaaa catcattaac aacacatccc
     7321 caatcataat atccattttc aaatacaacc cctcggatga gatctacacg ctccagtcca
     7381 accaatcctt tagttgccat cgattccgat tttttaccat cgaaatagca ccatatccca
     7441 caacaaactc agttgtactg accccttctt caattttctc tgaatccctc acatataatt
     7501 ttcctttcgg atcatcagtt aagaccaact cataaagttc atcttttatc attcggaata
     7561 aatgtgctgg aatccttttt ttagtttcac tcagcacctc aaatatttct ttataggatg
     7621 gtgcctgaac atattttacc gggatttttt tatgaatcac agaatcacta atatcagcct
     7681 cttcaagttc aggtttccat tcaacaaaca ataatttatt ctgtaaaaaa tccaggtaat
     7741 gactccaact tactgatagt gttttatgtt cagataatgc ccgatgacct tgtcatgca
//
LOCUS       DAJTNA010000175         7749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTNA010000175 DAJTNA010000000
VERSION     DAJTNA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.177"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..795)
                     /locus_tag="OO161_003354"
     CDS             complement(<1..795)
                     /locus_tag="OO161_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN/FAD transporter"
                     /protein_id="HCR5464313.1"
                     /translation="MLRHILTAKNLLSNPIFNFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPFAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFCEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIISGILIYG"
     gene            800..875
                     /locus_tag="OO161_003355"
     tRNA            800..875
                     /locus_tag="OO161_003355"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:833..835,aa:Asn,seq:gtt)
     gene            complement(913..1863)
                     /gene="cbl"
                     /locus_tag="OO161_003356"
     CDS             complement(913..1863)
                     /gene="cbl"
                     /locus_tag="OO161_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5464314.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGISRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKSFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1965..2882)
                     /gene="nac"
                     /locus_tag="OO161_003357"
     CDS             complement(1965..2882)
                     /gene="nac"
                     /locus_tag="OO161_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5464315.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3209..3284
                     /locus_tag="OO161_003358"
     tRNA            3209..3284
                     /locus_tag="OO161_003358"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3242..3244,aa:Asn,seq:gtt)
     gene            complement(3341..4276)
                     /gene="ldtA"
                     /locus_tag="OO161_003359"
     CDS             complement(3341..4276)
                     /gene="ldtA"
                     /locus_tag="OO161_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5464316.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVKRKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4338..5417)
                     /gene="cobT"
                     /locus_tag="OO161_003360"
     CDS             complement(4338..5417)
                     /gene="cobT"
                     /locus_tag="OO161_003360"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5464317.1"
                     /translation="MQILADLLNTIPAIDSAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AEQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     QELAKNGVTLFGVGELGMVNTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGIDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSVEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5429..6172)
                     /gene="cobS"
                     /locus_tag="OO161_003361"
     CDS             complement(5429..6172)
                     /gene="cobS"
                     /locus_tag="OO161_003361"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5464318.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGESILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6169..6714)
                     /gene="cobU"
                     /locus_tag="OO161_003362"
     CDS             complement(6169..6714)
                     /gene="cobU"
                     /locus_tag="OO161_003362"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5464319.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQILYIATSQILDDEMAARI
                     EHHQQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2130 a         2042 c         1664 g         1913 t
ORIGIN      
        1 gccgtaaatc aatatgccgc taataatatt aagaatattc aggctaccgt taatcagtag
       61 cggtattttc gtattccctg caccacgaag tgccccgcta ccaattagag tgatggcagc
      121 tgctggataa ctgagtaccg tcagctccag ataagtcaac gccagtgctt taacttctgt
      181 cgtggcatca cccgcgacga aatcaataat ttgctcgcaa aaatgatgaa taagcgttgc
      241 caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg
      301 tcgtcgatcc cgcttaccga gactaaatgc cacaacgaca gtagtaccaa gatcgatagc
      361 agcaaaaaaa gccataatga ccatattgaa gctgtccgcc aatcccacgc cggccatcgc
      421 atcttttccc agccagctga ccagaaaagt gctcagaacc cccatcaaca ggacacaggc
      481 attctccatg aagataggaa cagcaaacgg ggttatctcg cgccagaaca acactttgta
      541 gctcttgcgt ttagcgtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat
      601 attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaataatg agggagaatc
      661 agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag
      721 aaacggcaaa cagttgggaa aattaaaaat cgggtttgac aaaagatttt tcgccgttaa
      781 gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
      841 gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata
      901 gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctgccg
      961 cttgatatcc tcaactgaaa gtcctgcatt acaaagttcc agaaagcgcc agacatagtt
     1021 acgctgaagt tgtcctcgct tcagtcccaa ccagacagta ttagcatcaa aaagatgccg
     1081 cgtatccagg cggattaaat tcttttcctc ttgttcaccg ctggattgtt cggcaactat
     1141 tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct gcgcacttaa
     1201 tacaatatct gccagcaaac ctttgcgggc aaatgcgtca tcaatacgtg agcgccccgt
     1261 aatcccctgt cggtaagtga ttaacggcca cttcgctatt gattccagcg tcaatggtgt
     1321 aatttgcgtc aagggatgat cgagtggaac aagcaaacta tggtgccaac gaaaccacgg
     1381 gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc
     1441 accattatgc aacaacgtcg caatttcctg tggcgtcccc tggattagct cgagccgaac
     1501 ctccgggaaa agttcgcgaa aagatttaat gacctctggc aaactataac gtgcctgagt
     1561 atgcgtcgtt gcaatagtga gaacgccaga cgtatcgttg gtaaacaggt ctgcaagccg
     1621 acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg
     1681 ttcagtcatg cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caagttcatc
     1741 ctccagttcc cgaatatgac ggctgatgcc ggactgtgag gtaaaaagca tattcgcaac
     1801 ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt gttggaaatt
     1861 cacggtaaac tccgggcagt tcagattttc cgttattgtt aaagtctaat gcccggcata
     1921 acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct caccaattgc
     1981 cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc
     2041 tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta aggacaaaga gagactcatt
     2101 gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga acgcgcagcc
     2161 gattcaggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata
     2221 gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaaagc ctcatcaaca
     2281 cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgcgc aatagcatta
     2341 acatcaacgc tttgccccgg gcaatcttga gttcctacca ggaaaagatc ttctttcagc
     2401 aaagcctgac tggatacccc agccactggg gaatgctcat aaatcaccgc catatcgagt
     2461 tggtgattta tcagtttttc gttaagcact gcaccactat tttcatgaag atagataacg
     2521 acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg
     2581 gttcctggcg caaagccaat cgagacttgc cccgataatg actgaccaac gttatgcacc
     2641 gccagttggg cctgctcaca ctgacgtaaa atggcccgtg catgggtata gagaattttt
     2701 ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg atttaactca
     2761 ccttccagtg tggcaacctg ctggctgagc gcaggttgtg cgatatgcaa tacttcagca
     2821 gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc
     2881 atgttgcctc cggtttttaa gaatcggccc aagtgccgcc attacttaca accagaaagc
     2941 aagatgcttg ccagttctat tttggcgttg atgcgcaagc taaccaactg tcaaataaaa
     3001 gattatgata gattcgtcat tggccctttt tatcagcagt cgcattcacc tgccctataa
     3061 aaggagggta tgcaccacga tggttcatta cacaataaga ttgaaagctc accactttgt
     3121 tgaaattggc agcaaacaaa caaaaaaata catttcaccc tttgacatca ccatgcactg
     3181 ccattaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
     3241 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaaagcct
     3301 gctttcgagc aggcttttta ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     3361 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     3421 catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     3481 ttcggtcaga ctcatattct gcacgattgc gcgacagcgg ctcatgaact tccagccact
     3541 ttgaaccatc cggttccgtt gtgtatttca ctggctgatc aataatttgt acacgagtgc
     3601 ctacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     3661 gacttacccg gagcccaata ccaaaattgg cattggtacc gtggatggca tacaacctgc
     3721 caatataaat cgcgtacagc cccatggggt tatcgggccc cgcaggaaca aatgcgggca
     3781 aactctcccc tcgtttcgca tattcgcgcc gggtgttcgg cgttggcgtc caggttggtg
     3841 cttcttgttt acgtttaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     3901 cgataccaat aggaaagact tccactgtgt tactgtctgg cggatagtaa taaagacgca
     3961 tttcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     4021 gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc agcgcccgga ttcgcttcca
     4081 gcatattact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgagtattgt
     4141 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     4201 gataagttac cgccaggcta ttttggctgg caaaaagcag agcaaatgag caaagaatat
     4261 ttacacgacg catcatgtcc ctttcctaag tcgcgaatac tatccgataa gtatagcttt
     4321 tatcagactt ttcgttttta gctgttcaaa tcagaagtcg tattccccgg tagaacaata
     4381 ttactggcag caagttcgcc catgttgttg tatatcgcac aagcagcttc gatgatgggc
     4441 atcgccagag ctgcaccact cccctcacct aaacgcatat ccatattgag ataaggctcc
     4501 agccccaaat gcgagagcgc tatacgcgcg cctttttcta ccgacaagtg agaaggaatg
     4561 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcagcagc ataagaaaga
     4621 aatccatcca gcaacacggg taaaccgcag gaagcagcac ctaacatcac tccagctatt
     4681 ccgaccaaat caaatccacc cacttttgcc aggacatcaa taccatcctg aggatttggt
     4741 tgattcaacg taatcgcccg acgcacaaca tcaattttat tagccagttt atctgtcggc
     4801 aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt gctgactatt
     4861 gctgctgccg gtgtcgtgtt taccatcccc agttcaccta caccaaacag cgtgacaccg
     4921 ttttttgcca gctcctgcgt ataacatatg acgtccaaaa gcaacttttc agcctgacgg
     4981 cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg catgttgata
     5041 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtggac gttagcgccc
     5101 gcttgttctg ccagcacaca cacgccggtt gttccacggg tcatattttc agcctgtatg
     5161 gctgtcactt cttttggaga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     5221 ataaccagta ccgctttttt gcccacatgc ggtatgccat tcaaccccgg cattcctgcc
     5281 agttgtatgg caagcgcctc cagctttccc aggctaccaa caggtttgag taacccgtca
     5341 atatgccgtt gtgcacgcga catagcggca gaatcgatgg ctgggatcgt attcagtaaa
     5401 tcggcaagta tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     5461 caagctctat ggctgctccc agcgtatcgc ccgtttgccc gcccagcgta cgtttgagaa
     5521 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     5581 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     5641 catcaatttt gccaatgaac acattgccaa gcccttcttc ccgcgcataa cgatgacgat
     5701 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     5761 tcgattcgcc acgcagagcc aactcgctta acaccagaat ctttgccagt accacaaaaa
     5821 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     5881 cgcggctacg tgcagaaaat acgccatcac aggtatcagc gaggccgtcc agatggaatc
     5941 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     6001 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     6061 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     6121 gtacaggcag acgcgtaata aatgagagca tcgcccaaaa taatttactc atttgatttt
     6181 tactccaata cccgaaacca ccagccatac ttcatttgcc gcagcggcca gctgctgatt
     6241 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     6301 tcccacttcg ttagtcacta atacaacctt tgcggggcaa cgttggcagg cagcaatcaa
     6361 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     6421 tttatcgccg ccataatcaa acaacagatt agtcaccatt gtggtaacgc attccagcaa
     6481 cacaacctca ttcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     6541 cactgtgcgc cagtgctccg ggcggccttg ctgatgatgt tctatccgtg cagccatctc
     6601 atcatcaagg atttgcgagg tagcgatata cagaatctgt gaagagtccc caataagcgc
     6661 ctctgcgtgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     6721 tcctgattca aatgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     6781 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     6841 caatccatta cgaaataaga tggcattacg gaatagccag actttctcgt gttaacgtcc
     6901 gacaaaatgc gtcattgaca gcggtttctg tggattgaca catttcattc agataatgaa
     6961 ttaatgctac tgccggacca accagcaaac gggcattgtg ttctgaaatc caggctattg
     7021 attcaacgtc agcgacagat ctgcgctgta aaagaacttg taaaacaacg caatagaatt
     7081 atccggaatg gtggcgacta tgcactagaa caccacaatt tcgctctctc ggtaaggcac
     7141 tgtcacagca ttcaacagaa tgtgacttgc cagatttatt agcgccacca gatgtttaac
     7201 cgggtaacca cgacccagtc ggggatatat tccatcttct cgctgacatc atcaagaata
     7261 aaaaggttta tcacactcag agcaacgttc ttcagcaggc gggataattc ttcaacattt
     7321 agaatatgtg ttattaaagg catacaaact ggacgtattg tattttcttt tggtggttga
     7381 cctaaggtag cagtttatcc tgatgcgctg agatttctgt aatatctacg tcaacattct
     7441 cctcgaatag aaaatgcagc cccggcaagt gattcacatt ttttaacaaa acgttgttag
     7501 cgtgccattt tcagaagctc tccaagaaag cgaattcttt tctcaaattc tgcattctca
     7561 gacacagctt tctactccat ggcaatggcc caaatacgaa gttgctcagg atcattagtg
     7621 gtgctgagaa gttaaataac catgcggagt accactcgtc atcacgtatt tatatatcat
     7681 gaagctattg ttgtgttttg tacatcagta gatgtattct gatatactcc ttttgctaga
     7741 cataacctt
//
LOCUS       DAJTNA010000176         7728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTNA010000176 DAJTNA010000000
VERSION     DAJTNA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.178"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..731)
                     /gene="pdeC"
                     /locus_tag="OO161_003363"
     CDS             complement(<1..731)
                     /gene="pdeC"
                     /locus_tag="OO161_003363"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5464320.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHL"
     gene            1161..1442
                     /locus_tag="OO161_003364"
     CDS             1161..1442
                     /locus_tag="OO161_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5464321.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(1541..2077)
                     /gene="ssb1"
                     /locus_tag="OO161_003365"
     CDS             complement(1541..2077)
                     /gene="ssb1"
                     /locus_tag="OO161_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5464322.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            2331..5153
                     /gene="uvrA"
                     /locus_tag="OO161_003366"
     CDS             2331..5153
                     /gene="uvrA"
                     /locus_tag="OO161_003366"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5464323.1"
                     /translation="MDKIEVRGARTHNLKNINLIIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHAKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(5188..5544)
                     /locus_tag="OO161_003367"
     CDS             complement(5188..5544)
                     /locus_tag="OO161_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5464324.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPGRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(5548..5964)
                     /locus_tag="OO161_003368"
     CDS             complement(5548..5964)
                     /locus_tag="OO161_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5464325.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(6075..6788)
                     /gene="aphA"
                     /locus_tag="OO161_003369"
     CDS             complement(6075..6788)
                     /gene="aphA"
                     /locus_tag="OO161_003369"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5464326.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKSGRNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            7190..7394
                     /locus_tag="OO161_003370"
                     /pseudo
     CDS             7190..7394
                     /locus_tag="OO161_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2006 a         1871 c         1890 g         1961 t
ORIGIN      
        1 ataagatgat tataaaaatg ggttataaga cgttgatatg aagtcgcaac aatggctgca
       61 attgggcgtt ttgtcgaata aactgtcgca tataaatagc catttctttg tacggttaaa
      121 tccttcaaat gaatcagcgg agaaaacgtt gctgccgagg cctctttgct taacgagaaa
      181 aaggttttcg tcaccgtatc atacacaccc cattgcaatg tcggatcatc agacatcact
      241 tcactccaga agagagggtt gataaccgcc acataatttc cccgctgcat ataggtcatt
      301 ttatagccag agaaaaaagg cgtatcgcgg taataataga tagaaacgtt aggttcacgc
      361 ttataatcgg ccggtgcaat cgtatagccg tttacaggcg ctatcagcga tgagcataaa
      421 aaatggttat cacgggcata gatcaattca ttaatataaa gatagccacg aataatattc
      481 aacattcgct tttgatgggc tggagtgcaa acttgcccct gatagcgttc agctgcatcg
      541 ctcactaaat ctgcctggcg aatgaccagc tcggatttat ccagtgccag ttgagcaaag
      601 gttcggagct gattattcac ttctgatttt gcccaaagga aggcaatcca tagcgaaaga
      661 atgatgggaa agagaactaa aaagataatg cccggcaacg ccagtaattg gtgtcgtgca
      721 cgatgactca ttgctatcaa tttcctgcct gtttataccc ggccattgta aggtgaaaac
      781 cctgggtacc gacgtcttta gataaatctt acggtataaa tagcactttt gcctgatttg
      841 aaattgatac ctttctaatc gtcacctgga tgaaaataat tcattgatat tcaaaattaa
      901 tttcctacag gaatgcagag gcggcgggaa gatatgccct taatgccact gaaaaaccac
      961 ataatctgta cagcaataaa agtcacggcc taatatggct aacaaacaat caattgtgat
     1021 atatttcaca aaattaatga aacaaacaga gtgtttcatt tttgtgttct gcaagccccg
     1081 cgatagccag agtgtaccac gcctcccgtg aacaacgccg cgctgtccag ggcatcggct
     1141 cttttcagag gaattgtttg atggctaccc tcaccactgg cgtggttctt cttcgctggc
     1201 aacttcttag tgccgtaatg atgtttctgg ccagcacact caacatccgt tttcgtcggt
     1261 ctgattatgt cgggcttgcg gtgatcagca gcggtctggg cgttgtttcc gcctgttggt
     1321 tcgcaatggg gttgcttggc atcacaatgg cggacatcac cgctatctgg cacaacatcg
     1381 agtcggtgat gatagaagag atgaatcaga caccgccaca atggccaatg attttgacct
     1441 gatacgtaga agcctccata acggaggctt cttttttatg gctggcagat gctttaatca
     1501 tccaccttaa aacaatataa cctattgttt taatgacaaa tcagaacgga atgtcatcat
     1561 caaagtccat cggcggctcg ttagacggcg ctgccggagc ggactgctgc gggcgagact
     1621 gcgcgccgcc gctgaactga ttgccaccct gcggctgctg aggctgaccc caaccgccct
     1681 gcggctgacc accaccgata ttaccacctg ccggagcgcc accaccctga cgaccaccca
     1741 gcatctgcat ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg
     1801 attgatcggt ccatttacgg gtacgcagct gaccttcgat ataaacctga gaacctttac
     1861 gcagatattc gctggccact tctgccagtt tgccgaacag cacaacgcgg tgccattcag
     1921 tctgttcttt catctcgccg gtcgctttat cacgccagga ttcggaagta gccagcgtaa
     1981 tgttggcaac tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac
     2041 caacgagaat aaccttgttt acgcctctgc tggccatgtt cgtgtctcct gaaaaaaatc
     2101 gttctgaata agtgtaaatg cgcgattgta ccattaccaa tagcgctttt actatgttgt
     2161 gacctcggtt ccgggaaaca aacctggcca gacattgtta cacaacactc cggataatgc
     2221 attccaatac tgtatattca ttcaggttaa tttgtgtcat aattaaccgt ttgtgatcgc
     2281 cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga
     2341 tcgaagttcg gggcgcccgc acccataatc tcaaaaacat caacctcatt atcccccgcg
     2401 acaagctcat tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca
     2461 ccttatatgc cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt
     2521 ttctgtcact gatggaaaag ccggacgtcg atcatattga ggggctttct cctgccatct
     2581 caattgagca gaaatcgacg tctcataacc cgcgttctac ggtggggaca atcaccgaaa
     2641 tccacgacta tttgcgtttg ttgttcgccc gcgtcggcga accgcgctgt ccggaccacg
     2701 acgtcccgct ggcggcgcaa accgtcagcc agatggtgga taacgtgctg tcgcagccgg
     2761 aaggcaagcg tctgatgcta ctcgcgccaa tcattaaaga gcgcaaaggc gaacacgcca
     2821 aaacgctgga gaacctggca agccagggtt acatccgtgc tcgtattgat ggcgaagtct
     2881 gcgatctttc cgatccgccg aaactggaac tgcaaaagaa acacactatt gaagtggtgg
     2941 ttgatcgctt caaggtgcgt gacgatctta cccaacgtct tgcggagtcg tttgaaaccg
     3001 cgctggagct ttccggtggt acagcggtag tggcggatat ggacgacccg aaagcggaag
     3061 agctgctgtt ctccgccaac ttcgcctgcc caatttgcgg ctacagtatg cgtgaactgg
     3121 agccacgact gttttcgttt aacaacccgg caggtgcctg cccaacctgt gacggccttg
     3181 gcgtacagca atatttcgat cctgaccgcg tgatccaaaa ccccgagctg tcactggctg
     3241 gcggtgcgat ccgtggctgg gatcgccgca acttctatta cttccagatg ctgaaatcgc
     3301 tggcagatca ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc
     3361 ataaagtggt gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc
     3421 gtggcgatac ctccatccgt cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc
     3481 gttataaaga gacagaatcc agtgcggtac gtgaagaatt agccaagttt atcagcaatc
     3541 gcccatgcgc cagctgcgaa gggacgcgtc tgcgtcggga agcgcgccac gtttatgtcg
     3601 agaatacgcc gctgcctgct atctccgaca tgagcatcgg tcatgcgatg gaattcttca
     3661 acaatctcaa actagctggt cagcgggcga agattgcaga aaaaatcctt aaagagatcg
     3721 gcgatcgtct gaaattcctc gttaacgtcg gcctgaatta cctgacactt tcccgctcgg
     3781 cagaaacact ttccggcggt gaagcccagc gtatccgtct ggcgagccag attggtgcgg
     3841 gcctggttgg cgttatgtat gtactggacg agccgtctat cggcctgcac cagcgcgata
     3901 acgagcgcct gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg
     3961 tggagcatga cgaagacgca attcgcgccg ctgaccatgt gatcgatatc ggtcctggtg
     4021 cgggtgttca cggcggtgaa gtggtcgcag aaggtccgct ggaagcgatc atggcggtgc
     4081 ctgaatcgtt gaccgggcag tacatgagcg gtaaacgcaa gattgaagtg ccgaagaaac
     4141 gcgttccggc gaatccagaa aaagtgctga agctgacagg tgcacgcggc aacaacctga
     4201 aggacgtgac gctcacgctg ccagtcggtc tgtttacctg catcacaggg gtttcaggtt
     4261 ccggtaaatc gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg
     4321 gtgcgaccat cgccgaaccg gcaccgtatc gcgatattca ggggctggag catttcgata
     4381 aagtgatcga tatcgaccaa agcccaattg gtcgtactcc acgttctaac ccggcgacct
     4441 ataccggcgt gtttacacct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc
     4501 gtggttatac gccaggacgt ttcagcttta acgtccgtgg cgggcgctgc gaagcctgtc
     4561 agggcgacgg tgtgatcaaa gtggagatgc acttcctgcc ggatatctac gtgccgtgcg
     4621 atcagtgcaa aggtaaacgc tataaccgtg aaacgctgga aattaagtac aaaggcaaaa
     4681 ccatccacga agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgcggtgc
     4741 cagctctggc gcgtaagctg caaacgttga tggacgttgg tctgacgtac attcgcctgg
     4801 ggcagtccgc aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc
     4861 tgtcaaaacg cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc
     4921 acttcgccga tattcagcaa ctcctcgacg tgctgcataa actgcgcgat cagggcaata
     4981 ccattgtggt gattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc
     5041 tgggaccaga aggcggcagt ggcggcggcg aaatcctcgt ctccggtacg ccagaaaccg
     5101 tcgcggagtg cgaagcttcg catacggcac gcttcctcaa gccgatgctg taatcgttaa
     5161 ggccgctttc tgagcggcct tttcctttca gagttgtacc agcaatttac gcttttcttc
     5221 cggtagtaaa ttcactgcct gctgataaga cgcatccacc agataataga tttgcgaatc
     5281 cggcagcgaa ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggccagg
     5341 acgcacatcg ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga
     5401 aacagctggg cgattttcta cttctttcac catcgcaaac agtacatctt ccactttgat
     5461 ctgcgtcgct ttccagtcgt tatgcacgct ctgttctgcg cctggttttg ccatgcaata
     5521 ttgtagcaac tccgaaatgg tcatttttta ctccccttgt agtgtcgcga tgatgcgacg
     5581 cgatccgccg tggatgcgat gttcccccag ccagatgcct tgccaggtgc cggtctgaat
     5641 acgcccttta tgtaacggca atacaagcga tgttccctgc attgaggatt tgatatgaga
     5701 aggcatatcg tctgctccct catagtcatg ctcataattt ccgttgtcgg gaacggtgcg
     5761 gaggaaaaaa cgctccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag
     5821 agaggcggag gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc
     5881 agccagctga ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag
     5941 cgtgagcgtc ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg
     6001 cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagcatcac
     6061 gctctcccgc tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct
     6121 tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgccg
     6181 acatcgcgtg cggcggtaat atcattatca gaatcgccat aaaaaattcg gatattttta
     6241 tcctgcagcc attgcgattt tgtatttcgc cctgatttat cgcccgcaaa gatcaccgga
     6301 ttcatgttgg tggcaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt
     6361 ttcgtcggac tacgaccagt cacaaagaag atcgcgtcac cgcggcgtac atgcatatca
     6421 atcagctggc gagcgacctc ttttggaatg ctgaattcat cccagccatt gttcattttt
     6481 tcccagaaca caggattttt cagataatct tcgctttctg gcgagaaggt ttttttgccg
     6541 cgccagaagc ccgggctgga aaaaagtacc gtgtcatcga tatcaaaccc caccgccatt
     6601 ggcggacgcc ctgcgaggct attttcaatt tgtgcgaccg aaacccaatg aattggtgcc
     6661 tgttcagcaa gcctggcaac gttagtacca gggttaagcg gtgaaggaga tgaggccagg
     6721 gcaacagcgg aactgtttag agcgaacaat aagcaaacgg cactgattgc ctgtgtgatc
     6781 ttgcgcatat ttttccctaa atagtcagta tgttgaaact ttttattgtg agatttgttg
     6841 caaaaaccat ctgaccataa cgccagctgc aggggaaagg aaggattatc tgcggttttt
     6901 gtgatgaaaa gcaggatgat tatatttaac ccagtaaata cgttcctatc tttttttgta
     6961 taaaaaatgt ttattactcg atgtaaggat acattaattt tacgtttatt atgatgaatc
     7021 ctctgcaaat gagcgaaatt tgatagaaca tttatctgct gtacgattta atgaacttaa
     7081 tgatacagca ttacaaaaac aatcgaagtt tataaagatg attcctgatt gaccagaacc
     7141 ctatcctaca atggtgatta tttttattac tcgcgatgga gatgatgata tgaatatatc
     7201 atatatgaat agcaacaaaa cgacatcttt acctgttgag ctagatgcac taaataataa
     7261 agatttttct tatgtcaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
     7321 agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     7381 atttatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg cacgctttgc
     7441 tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt atttgatatt
     7501 ggtaatcaat ataaggttaa acttacacaa agcggaaaaa aagagttctc tttaacatta
     7561 gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc aaagcatatt
     7621 cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta aatttaataa
     7681 tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaag
//
LOCUS       DAJTNA010000177         7724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTNA010000177 DAJTNA010000000
VERSION     DAJTNA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.179"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..2301)
                     /gene="nifJ"
                     /locus_tag="OO161_003371"
     CDS             complement(<1..2301)
                     /gene="nifJ"
                     /locus_tag="OO161_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5464327.1"
                     /translation="MITIDSNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRIIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSPDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKA"
     gene            2575..2841
                     /locus_tag="OO161_003372"
     CDS             2575..2841
                     /locus_tag="OO161_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5464328.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(2838..3260)
                     /gene="hslJ"
                     /locus_tag="OO161_003373"
     CDS             complement(2838..3260)
                     /gene="hslJ"
                     /locus_tag="OO161_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5464329.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(3371..4360)
                     /gene="ldhA"
                     /locus_tag="OO161_003374"
     CDS             complement(3371..4360)
                     /gene="ldhA"
                     /locus_tag="OO161_003374"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5464330.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4568..7207
                     /locus_tag="OO161_003375"
     CDS             4568..7207
                     /locus_tag="OO161_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5464331.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTMAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            7204..7389
                     /locus_tag="OO161_003376"
     CDS             7204..7389
                     /locus_tag="OO161_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5464332.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKDPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            7394..>7724
                     /locus_tag="OO161_003377"
     CDS             7394..>7724
                     /locus_tag="OO161_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5464333.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSIDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKLV"
BASE COUNT         1884 a         1878 c         2057 g         1905 t
ORIGIN      
        1 ggctttaatc tctggattct gacggtcttt cgccgggcaa acttcgacgc acaggttaca
       61 accggtgcaa tcttccggag ccacctgcaa gacatatttc tgcccgcgca tatcacgcga
      121 tttcacatcc ggcgaatgca ggctggcagg ggcgttttcc atcgcttcgg gtggtaccac
      181 ttttgcgcga atagctgagt gtgggcaagc ggcgacgcag tggttacatt gggtacagag
      241 ttcctctttc cagatgggga tctcttcggc gatattgcgt ttttcccagc gagtggtccc
      301 catcggccag gtgccgtctg gcggcagcgc cgaaacgggg agggcatcac caagccctgc
      361 gagcatcgct gcggttacgg ttttcacgaa atcaggggcg gcatcggaaa ccactggcgg
      421 tcgattggcg ctgtgcggat ttaccggttg caacggaact tcttctacgg attcacgcgc
      481 cagcgccaga gcctgccagt tgccttccac cagatcctgg cctttgctac tgtaactttt
      541 ggcaatcgca ccctgcaatt ctgcgagggc gctatcgcca ggcagaattt gcgtcagatg
      601 gaaaaaagcc atctgcatga cggtattaat acgggccgcc aggccacatt cgcgggcgat
      661 tttcgccgcg ttaatcacat agaagcgcgc ttttttctgg tttaacacgg cctgaacttc
      721 ttgcggcaag cgcgaccaca cttcatctgc gctgtacggc gtgttgagca ggaaaatacc
      781 gccaggtttt aaacgctcag ccatctgata tttatcgata aactgcaact ggtggcagcc
      841 aacaaaatca gcctgggaaa tgagataagc ggaacgaatc ggctgttcgc tcacgcgaag
      901 gtgagaaacc gtcaggccgc ccgctttttt ggagtcgtaa acaaaatagc cctgtgcgta
      961 ccacggcgtg gaattaccga taatcttgat attgtttttg gtcgcggaaa cgctgccatc
     1021 actaccaagg ccataaaaca aggcttcaag tttcgccgag tttggctggg tgttttccgg
     1081 caacggcagg gacagattgg ttacatcatc gtaaatacca accgtaaagc gcgctttcgg
     1141 tttagccgcg ttgagctcgg caaataccgc cagtacacag tccgggccaa attctttgga
     1201 tgaaagacca tagcgcccac caatgatacg gggcagagtt tcgcgcttgc cattattaaa
     1261 ggcttctgcc agtgcggtca tcacatccag atagagcggt tctgcctggg caccgggttc
     1321 tttggttctg tccagtaccg ccacgctgcg tacggatccc ggtagagttt gtagcaagtg
     1381 tttggctgag aaggggcggt acaggcgaac tttcagcacg ccgacttttt cgccacgggt
     1441 tagcaattca tcaaccactt cttcacaggt gccaatggct gagcccatca ggataatcac
     1501 ccgttcagct tgcggatgcc cgtaatattc aaacggctga tactgacgac ctgtcgcggc
     1561 agagaaatca ttcatcgcct gttcaacatg gtcatagact gcgttgtacc aggggttggt
     1621 ggcttcgcga gactggaaat aagtgtcagg attggcggac gtaccacgga tcaccggatg
     1681 ttccgggttg agcgcccggg cgcgatgagc atcaatttcg gcctgcggca tgagatcaag
     1741 aatcgtgtca tcggccagcg ggactatttt attgatttcg tgggacgtgc ggaaaccatc
     1801 aaagaaatga ataaatggca cgcggctttt cagcgtcgcg atgtgcgaaa tgagagcaaa
     1861 gtcttgcgct tcctggacgt ttgccgcaca caacatcgcg caacccgtct ggcgcaccgc
     1921 catgacgtcg gaatgatcgc caaaaataga gagtgcatgt gtggcaacgg tacgtgccgc
     1981 tacatgcagg acaaacggtg tgagttcgcc tgccagtttg tacagtgtcg ggatcatcag
     2041 cagcaaaccc tgcgatgacg taaacgatgt tgaaagggca cccgtctgca aagcgccatg
     2101 cacggtagcg atagcacccg cttccgactg catttcaacc acgcgtggtg tgtctcccca
     2161 aacgttcttc aagccgtttc cggcccaggc atcagcctgt tctgccatcg tggaactggg
     2221 ggtaataggg tagatggcga taacttcact ggtgcgaaac gccaccgaag caaccgcgcc
     2281 attactgtca atagtaatca tatgacaccc ttacattgcg caaatgaggg gcgcacgaaa
     2341 ttgctgcgcg cccagtagta atctttcaat tttagcaaat ggctttcttc tgcattttcg
     2401 cttttgtgtc ccccacatca gcgtaatgaa tgttttgatc aaacagaggg caaaaaaata
     2461 gccataaaaa agtaaaaatg cggggcaacg cgcaggacag ctctcgatgt cgccctctgt
     2521 gctgcctatt atttatggcg gtgtcgtttg gcttgaagat cagaggaaga aaagatgcga
     2581 gcagcgtttt gggtagggtg tgccgcttta ttgttgtcgg cgtgtagtag tgaacctgtt
     2641 cagcaggcga ctgcggcgca cgtagcgcca ggtttaaaag cgtcgatgtc cagtagtgga
     2701 gaagcaaatt gtgcaatgat cggcggttcg ctttctgttg cccgtcaact ggatggcacg
     2761 gcgattggga tgtgtgcatt acccaacggc aaacgctgta gcgaacagtc acttgccgcc
     2821 gggagctgtg gcagctatta attcattaaa tccgccagct tataagttaa tgtctgtttc
     2881 gcggtcgcca gcgttaactg gttcgcggtc agatccactt gtgcaccttc tttcagcatt
     2941 tcgctaatgg tgttatcgag ttcattaagc tgcgggttag cgcacatcat acgggtcatt
     3001 gccagccctt tggctgtcag ttcaccatta gacagtttgc cttcaccgct aaagcggtta
     3061 cacatgctgc cggaaatcat cattttttca ccaaagctga tttctggcgg atttttatcg
     3121 ctggtcacgg gcttaccgtt tacgctttcc agcacaaagc gatgatgctg tagctgttct
     3181 ggcgtaacag caattttgtc attacttaca catcccgcca tcagcaggct tagcgcaaca
     3241 aacgcggcta ctttcttcat tgtggttctc aattacagtt tctgactcag gactatttta
     3301 agaatagaga atgaaaggtc attggggatt atctgaatca gctcccctgg attgcagggg
     3361 agctgcaaca ttaaaccagt tcgttcgggc aggtttcgcc tttttccaga ttgcttaagt
     3421 tttgcagcgt agtctgagaa atactggtca gagcttctgc tgtcaggaat gcctggtgcc
     3481 cggtaaacag cacgttgtgg caggcagaca ggcgacggaa tacgtcatcc tggatcacgt
     3541 cgttggattt atcttcaaag aacagatcgc gttcgttctc atacacgtcc atacccaacg
     3601 aaccaatttt ctgatttttc agcgcttcaa ttgctgcctg agaatcaatc aatgcaccgc
     3661 gactggtatt gacgatcatc acgccatttt tcatctgatc gaaggcggct tcgttcaaca
     3721 gatggtagtt ttccggtgtc agcgggcagt gcagagagat aacgtctgat tcagagaaca
     3781 gggttggcag atcgacatac tccacaccga gttccagcgc cgctgcactt ggatacggat
     3841 cgaacgccag cagacgcata ccaaaacctt tcagaatgcg cagcatcgcc acaccgattt
     3901 taccggtacc gataacgcct gccgttttgc catacatagt aaagccggtc agaccttcca
     3961 gagagaagtt agcgtcacgg gtacgctgat acgcgcggtg aatacggcgg ttcagcgtca
     4021 tcatcatacc gatggcgtgt tcagcaacgg cctctggatc ataggctgga acacggacta
     4081 ctttcagccc cagttctttt gccgcatcaa ggtcgacgtt attgaaaccg gcacagcgca
     4141 gggcgatata tttaacgccg tgctttttca gctcttccag caccgggcgg ctgccgtcat
     4201 cgtttacgaa aatacatacc gcttcgcagc cattggcagt tttagcggtt ttttccgtca
     4261 gcagaaagtc aaaaaattcc agctcaaagc caaaggactc gttcacctgt tgcaggtact
     4321 tcttgtcgta ctgttttgtg ctataaacgg cgagtttcat aagactttct ccagtgatgt
     4381 tgaatcacat ttaagccact aaaaatattt tacaaaattt caaatataat tgaaaaccat
     4441 ggcgatattg aaaaattcat caacaactat gcttagtgta ggcgcaacct tcaactgaac
     4501 ggttaaacat gccacaatac ccgtattgaa tgcttaattt ttcgctaaat caggatatta
     4561 actacccatg ctgggtaaat ataaagccgt tctcgcgctg ttattactga ttattcttgt
     4621 gccgttgacg ctgctgatga cgctcgggct gtgggttccc acgatggcgg gcatctggct
     4681 accgctcggg acacgtattg cattagatga aagcccacgc attacgcgta aaggtttaat
     4741 cattcccgat ctccgttatc tggtgggaga ttgtcagctt gcgcatatca ccaacgccag
     4801 cctttcacat cccagccgct ggttattgaa cgtcggcacg gtagaacttg attctgcttg
     4861 tctggcgaaa ttgccgcaga cggagcaatc gccagctgct ccaaaaaccc tcgcgcagtg
     4921 gcagtccatg ctgcctaaca cctggatcaa tatcgataaa ctgatttttt ctccctggca
     4981 ggaatggcag ggaaaactct ctctcgcatt aacctctgat atccagcaac tgcgttatca
     5041 gggcgaaaaa gttaaatttc aaggccagct gaaagggcaa caacttacag tcagcgaact
     5101 ggatgtcatc gcgtttgaat atcagccgcc ggttaaactg gtgggggaat ttactatgcc
     5161 gctcgtgccg gatggacttc ctgtaagtgg gcatgctact gcgacgttaa acttgccgca
     5221 ggaaccgtca ctggtggatg cagagctgga ctggcaggaa aatagcgggc aattgattgt
     5281 gctggcacgt gataacggcg atccgttgct cgatttgccg tggcaaatta ctcgtcaaca
     5341 attgaccgta agcgatggtc gctggagttg gccgtatgca ggttttcctt tgagtggccg
     5401 actgggtgtc aaagtcgaca actggcaggc agggcttgag aacgctctga tcagcggacg
     5461 actgagtgtg ctgacccagg ggcaagcggg taaaggcaac gcggtgctaa attttggccc
     5521 aggaaaatta agcatggata acagccagct gcctctgcag ctgaccggtg aagcgaaaca
     5581 ggcggacctc attttatatg cccgcttacc tgcgcagcta agtggaagtc tgactgaccc
     5641 aacgctggcc tttgagccag gcgcgttact tcgttcaaag ggaagagtca tcgattcgct
     5701 ggacatcgat gaaatccgct ggcctttagc gggtgtaaaa gtcacccaac gtggtgttga
     5761 cggacgtttg caggccattt tgcaggcgca tgaaaatgaa ctgggcgatt tcgtgctgca
     5821 tatggatggg ctggcgaatg attttctccc tgacgcgggc cgctggcagt ggcgctactg
     5881 gggaaaaggc agttttacac cgatgaatgc cacctgggat gtcgcaggaa aaggtgaatg
     5941 gcatgacagc acgattacgc tgaccgacct ctccaccggt ttcgaccagt tacaatacgg
     6001 tacgatgacg gtagaaaagc cgcgattaat tctggacaag cccgtcgtct gggtacgtga
     6061 cgcacagcat ccctccttca gcggcgcgct gtcactggac gccgggcaaa cgctgttcac
     6121 tggcggcagt gtgttaccgc catcaacctt aaaatttagc gtcgatgggc gcgatccgac
     6181 gtatttcctc tttaaaggcg atttacatgc tggtgagatt ggcccggttc gggtaaatgg
     6241 tcgctgggac ggtattcgtc tgcgcggtaa cgcctggtgg ccaaaacaat cactgactgt
     6301 attccagccg ctggtgccac ccgactggaa gatgaactta cgcgatggtg aactgtatgc
     6361 tcaggttgca ttttctgctg cgcctgaaca aggattccgc gcgggagggc acggcgtgtt
     6421 gaaaggcggt agtgcctgga tgccagataa tcaggttaac ggtgtcgatt ttgtcctgcc
     6481 tttccgtttt gccgatggag cctggcatct ggggactcgc ggccccgtta cgttgcgaat
     6541 tgccgaagtg attaatctgg tgacagcgaa aaatattacg gctgatttgc aagggcgtta
     6601 tccgtggact gaagaagaac ccttgctgtt gactgatgtt agcgtcgatg tgttaggcgg
     6661 taacgtactg atgaaacaat tacgtatgcc gcaacatgac ccggcgctgt tgcggctgaa
     6721 taatctctca tccagcgaac tggttagcgc cgtcaatccg aaacaattcg ccatgtccgg
     6781 ggcatttagt ggtgcattgc cgttatggct aaacaacgaa aaatggatag tgaaagatgg
     6841 ttggctggca aatagcgggc caatgacctt gcggctggat aaagacaccg cagatgcggt
     6901 ggtgaaagac aatatgactg cgggttcagc aattaactgg ttgcgctata tggaaattag
     6961 ccgttcatcg acaaatatta atttagataa tctcggttta ttaaccatgc aggccaacat
     7021 tacaggtacc agtcgcgttg atggtaaaag cggtacggta aaccttaatt accatcatga
     7081 agagaatatt tttacgctgt ggcgcagttt acgctttggc gataatctcc aggcatggtt
     7141 ggagcagaac gcacgtctgc cgggaaatga ctgtccgcaa ggaaaagagt gtgaggaaaa
     7201 acaatgaaaa ttttactggc tgcgttgacg tcatctttta tgctggctgc ctgtacgccg
     7261 cgtattgaag tcgctgcgcc caaggatccg atcactatca atatgaacgt taaaattgag
     7321 catgagatca tcatcaaggc agacaaagat gtcgaagagc tgcttgaaac tcgtagcgat
     7381 ctttactgag gcgatgatga agaaaacatt acttctttgt gcgtttcttg ttgggctggt
     7441 aagcagcaat gtaatggcat tgactctgga tgaagccaga acgcaggggc gggtcgggga
     7501 aacattttat ggttatctgg ttgcgctgaa aacggatgct gaaacagaga aattagtaac
     7561 cgacattaat gccgaacgta aagcgagtta ccaacaactg gcaaagcaaa ataatgtgtc
     7621 tatagatgat atcgcgaaac tcgccgggca aaagctcgta gctcgggcca aaccggggga
     7681 atatgtacaa gggataaacg gtaaatgggt acgaaaattg gtaa
//
LOCUS       DAJTNA010000178         7707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTNA010000178 DAJTNA010000000
VERSION     DAJTNA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.180"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(262..696)
                     /locus_tag="OO161_003378"
     CDS             complement(262..696)
                     /locus_tag="OO161_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5464334.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(684..1085)
                     /locus_tag="OO161_003379"
     CDS             complement(684..1085)
                     /locus_tag="OO161_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464335.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(1345..1914)
                     /locus_tag="OO161_003380"
     CDS             complement(1345..1914)
                     /locus_tag="OO161_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5464336.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(2659..2802)
                     /locus_tag="OO161_003381"
                     /pseudo
     CDS             complement(2659..2802)
                     /locus_tag="OO161_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(3136..3402)
                     /locus_tag="OO161_003382"
     CDS             complement(3136..3402)
                     /locus_tag="OO161_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464337.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            3543..3749
                     /locus_tag="OO161_003383"
     CDS             3543..3749
                     /locus_tag="OO161_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5464338.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            3844..4446
                     /locus_tag="OO161_003384"
     CDS             3844..4446
                     /locus_tag="OO161_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464339.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            5646..6563
                     /locus_tag="OO161_003385"
     CDS             5646..6563
                     /locus_tag="OO161_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464340.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            6648..7520
                     /locus_tag="OO161_003386"
     CDS             6648..7520
                     /locus_tag="OO161_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5464341.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMCK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT         1871 a         2021 c         1863 g         1952 t
ORIGIN      
        1 actattttat ccctgttgaa tttggttaag ccggtgcaga cgcgccggga aacgcacacc
       61 tgaaccggca caataaaata caatacaacc ccctgttact ggagatacga atggaatgac
      121 gaggcagcct gtcagtcaca gggggctgtt ggcataaaat caacggaaag gccaccaaca
      181 ccggaattaa gctccacagt ccccgcactg gcaatccatt cccgggaaag gcatcaccgc
      241 aaactgctcc ggaggcaacc attacagcct ctgtaacgga ttatcagccc tggcatcttc
      301 cgacggcacc tcctgccgcc cccacgccct gctgtatacc ccggtaccgc agcgtgggca
      361 gcacttttcc ccctcataat cttcgtcaca catcacacaa aaccactttc tcaccttcac
      421 cgtataatcc ggaaccgtca tgccctgcag atgcaaacga cgggcctctt tctctgccgg
      481 gtcaagccag gtacatcgca gcaggatttt ttcagcgaca gtcatctggt catgttcaaa
      541 ccacggtggt tgtccgtcat tgccccgctg aagtgttaac gggcatccac aggacacaca
      601 gcacaaaacg tcctgtcggt tgtgcttcgc agcttctttc gccgtaacaa aatggccttt
      661 gctgtcattc gccatataac agttcagaat tcgcatattt cctcctgagt tgtccgggga
      721 atactgaatc ctccggtctg gcagtgtgtg cagtactgct ccccataata atcgtggtga
      781 cattgtctgc agtgccagct ggctttacgc accacgggta aggcatccgg tacgaattgc
      841 tgcagacgtt taatcaactg tatttctctg cgctccggcc tgacataagg gcactgttga
      901 ccgtgctctg tcagcctgtc gtcagtgtgt tcaaaccagg gaagttcagt ttcgtattgc
      961 ggatggtatc tgagtgcact gccacacaag tggcaggtgt agttagcata aggtgcggcc
     1021 tgtgcagtac gggcgcccgt cagacgtccg ttgccatcaa gagcgataaa ggattttgcg
     1081 tacatagtca tatacctcag ccgccagacg acacgcaggc aacagccgtc ccggtacggg
     1141 cagcgtggtc agggtgtgaa tggcggttca gttaaaatct ggactggagt ttgggtaaag
     1201 caggctggtt gctgttacag cactgtggtt gtcacacaat ggaacactga cgtaaaaaac
     1261 agtcagtggg cgagtggata catcgttgcc gccagacaac aacccatact tttggtttgc
     1321 cggatattgg cgcgccagtc gtgctcaact ccggctacag ccaaaattgc gctcaccggt
     1381 gcagtgagct ttggtatgct ccttcgccag atagtcagca cgttccagca cttgctgaaa
     1441 gccggtgtca tcattacgcg ccagccacac cgccggccag gccggaaaat gcaccagcgt
     1501 ggcatggcac ccgacatcca ctcccagggc actgcaccac gcctgtttaa tcatcccggc
     1561 cagagactcc ggagcgcggt aatcaccggc acgacaccag gtatcccggt tgaccagcag
     1621 catcaggtga tagtgttttt tacctttgca ctccccaaac tcccgggccc agacgtaatg
     1681 cagggttgtg ggatgcacgc gtttaccttc ccgatgctta cgtttctggt aagcgtcgat
     1741 tcgggctttc agggcattga tgaagcggga tatcacagct gcgtccgttg ctgccggtac
     1801 atccgggaga cgcaggtcaa cccgcaatgc cgtcaggcgg ggatgaacat tcagtgcgtg
     1861 ccgcaccgtc tcacgaatac gttgctgcca gaaggggttg tatttgtagg tcatggttaa
     1921 atctccgtat ggttcatacg gaatagccac gtcgtaaaaa atacgcagag tccctgaagt
     1981 gacctccggc aggacactgt ctcaggcact ctgtgataac ccgaacggtc gtctccggac
     2041 tgacggttga attttcagcg tcgtttttta atgtaaaaaa ccggttacgg gtaaggctgt
     2101 gaggagggag tgatggtgat acgcaaaaag aagtgcaggg actgtggaaa cgcgattact
     2161 cataatacag tgtgttgccc gtattgtggt tctgtcgacc ccttcggcta ctaccggaat
     2221 acagaccgta tcgttaccat actgctggcg ctgattattg tggtcctgct gactactgtc
     2281 agcgtcagtg tgtgtatact gtgtagctgg tgagataacg gttcatgtat cccctgagct
     2341 gacagtgtac cgtgtatatt aagcgtaacc gacaggttct ccttcatacc atattaaatt
     2401 ttccagggat tgccgctgcc ttatttcgtc ccatacagga aaactcatct gggtttctga
     2461 caaatacgcg aaactcacag cccctcaata tcaaccacac gttgtaagcc ggtaatacct
     2521 gaaaatcagc ctattaccgc ccacgcctcc ggcaccattc ccttttgatt tcgaattctt
     2581 tgttgtcgct ataccgctta atgttcccgg tcctccggtg cagataacac cggagtcatc
     2641 gtagcgctgc ttcaatattc aggtgacgta tttccagacg gaagccggcg cgcgatcgta
     2701 aagtttgttc attgtcagtt ctgccagccg gttgtcaacg gcagagttaa aggcttccag
     2761 ctccgcaagg gtaagtttat aacgcgtgtg ggaattcact tttcccgcgt ctcctgacgt
     2821 attacgggct gccacttacc tggtgtcccg caatacagcc ttagtgttcc gttatcgggg
     2881 atccggacgc accgcaacaa ccggggaagt atttctggtt tagagcagtg tcaagaggga
     2941 tttctcagcc cctcactcac cggctcagtc tcagggcagg agcattaata cgctcagttc
     3001 atgtcttata caggaatggg cacaggggga gctggcgtgt cactgctgat tttacgcatc
     3061 agcgttcatg tatctgcact ctgatacatg aacgattcac agcgctctgt taccggagag
     3121 atttactctc ttagcttact ctggcaaatc ctttacgtta ctctctgatg acttcttata
     3181 tttataatac caatcagaca gccttatccc atcggtgata tcagtctcct cctcgccagg
     3241 aacgggctga ctcactgtaa ctacctccga aaacaactct gcaattatca gaacgcctgc
     3301 ttctctccct gtcctcacga aaactatccc ctctttatcg cgcgtgcgtg cggaagcatc
     3361 ttttcgcaac aaccacccgg tataccggta cggctcagcc accgcaacct ccccgtttat
     3421 ctcctggcgg tcacattccc gattattttt tacgtttctc cccggttgtt atgccggtga
     3481 aggtggcgcg ctgttttcat gaccacaccg gctggtttaa caacatccgg aggaacattc
     3541 tcatggctac cccagtttca ctgatggatg accagatggt cgacatggca tttatcactc
     3601 aactgaccgg cctgacagat aagtggtttt acaggctcat cagggatggg gcctttccgg
     3661 ctcccatcaa gctgggccgc agctcccgct ggctgaaaag tgaagtggaa gcctggctac
     3721 aggcgcgtat tgcacagccc cgcccgtaat ttctgaccgt tatccgttca cccgcagcag
     3781 acgtaatccc cggcctgccg gcggcattct gctgcctgtt atatccccgt gaggaatatg
     3841 aaaatgaaac aacagtatca gacccgctac gaatggctcc acgaaagcta ccagaaatgg
     3901 ctgaccggct tcacccggca cgccgtatcc tggggcgtgt gtcatccgaa tatctactac
     3961 ttccataatc tgacgcccgg ttgggtgtca ttcaatggtg aaaaaccgga gattgccatc
     4021 gtaccccaga gcctgcaccg gctgatttat ggccctgaca aacgggccac gccgcccctg
     4081 gatgatgatc tgatagtgaa tttatgcacc agtgaacatc tgctggttca tcatccgatg
     4141 ctggaaggca ttctcctgtc tgaatgtgaa cgtctgaggc agcgttctct ggcaaataaa
     4201 ctgatcagcc tcttccgtca gtttggtggc acggagttgc gcctcaaact ggtctggctt
     4261 tgctggcttg atttaatgac cggaaacagc cttgaagact ggaaagagaa cctgaaacgg
     4321 aaatcagaaa aagagctgga ggaatggatc atcaaccgtc agaggcagag cgcagcactg
     4381 acggatctga tggatcagta cgtgctcctg gcataccgca caacggttga cgatagccgc
     4441 aactgatgcc ggttgcttcc ggaaggtgtg acaaaatacg gaaatgatga tgctcccggc
     4501 ccccatgctc acctgcagag cgtgttcgtc gtcatcatac tgagccatga cgggcgtctt
     4561 aagggcatcc tgtcattgct gagcaggcaa caccaccaga ggagccacgg acagggatgt
     4621 tctttacagc ccgatacacg ccaggttctc agcagactga atgtgcaggt gcggttattc
     4681 accagaaatg gcatgctgcg gtacataccc atcctggtga caatatgtac gtggcgacag
     4741 cgatcccggc actgtggaag agactcactc agcctgcagg atattctttc cggcatccgg
     4801 gaatgtccgc ctgaatgcaa acaggatgat atttgaatcc gtaatactgt tctttcatca
     4861 taaataattt atgcagatac agttaaccgt tacagtagcc ggtttgtttt accgggaaat
     4921 aatatccgtc agactgatac cggaacatat ctgaaacacg ttatgatgtt ttgttatcag
     4981 aaatgttgta tattcttatc cgtggcgaca gtttaatgcc ctctggtatc acctgataat
     5041 gtatatcctg cgatgattcg ttatatttac gggacacgcc tcttttcatt gtgctccttt
     5101 tcagacttca ttttccgtaa gtgttccccg gcataaagga ggtacctttc tggcgtttat
     5161 aactctcttt atcggtgacg ccggtaatac agggaatacc acaccgacaa acgaggtatt
     5221 aacagacgat atttcagtgt ccataatttt tctgtccggt taaataacgt cagtctgctg
     5281 ccctctgact gtaatcatca aagccggaat gcataccggg ttacaacgta aaaaaagtga
     5341 tgctggcccc cggtgcagct ggcgggcgaa acatgtttac ctttcaatac cccctgtctg
     5401 cagtctccac tcttccatcc tcttaagcta cgtcggccac atgagtgcac ttccatgata
     5461 acaggtattc ctgtcagcat aacgaagccg gttaccttcc cttcctcctg catttcctgt
     5521 cagaatgact atacagcgcg cagattgcct ttttattgac catcttaata taccgttata
     5581 aacttctttt tatcatatta ttatcacctt attatgttta atttttaagc agtcggggaa
     5641 agaggatggt tcagaaaatt ttgtcggata aggttatgaa tgaaagaaca aatgcgtatt
     5701 actcttatta tcttggagag cgtaatatct ccgtactgcc ccttaatgtt tatgatcccc
     5761 cggaacgttt tatcgcttac ataaaaaaaa acagagaaaa tctgaacatc acactttcag
     5821 attttgaact tgaacaaata atttctggta tgcgtctgaa ggccctggcc ttcctggtcc
     5881 ccctggaaaa aatatcctgg atcgcgggca gtgaacgcgc atgcttattt tcctggtatc
     5941 tgttgatgca gtttatccag aataacaggg caaaaataag cgcagacttg cttcaaaaga
     6001 ataaactgta tcttaaagaa gagtatctgg aagggaatgc cttcccgtcg gactcatcga
     6061 cacagttcag acaaatactc agagttctcg atattctttc tgataagaat ttgcgggatg
     6121 agtggataat ccagaccaaa gacagatgga tgcgggcatt taaatcaaag agtcctttca
     6181 gttacctcct cccggagaac gaacatgaat gcatatggac atggaattat ttgaagggaa
     6241 aaaatattgc actggaaaaa ctggccagtt ttcctggttc tgctgatatt taccacgcaa
     6301 ttcacctttc atttgacata tgggttacct gtccactcac ctcacctgac gatataaaaa
     6361 atttcagaaa cagtttcaat aaggccaagg cgcagcgcaa gtacaaaaaa atgcaggaag
     6421 ataaagtcaa tgtacagttt tttcttgacg cagaaaccag ggcgcagctc aaagaattat
     6481 cgagggtcag aagactcagt accggcgaaa tgctgcatga cctgattgtt gaagagtata
     6541 aaaggtaccg acattccaga taatttcaga catatattat ctccgtgagt cccccgccac
     6601 ctttccggtg tgcgggggtt ttgtcttttt tcaccgggaa tacatgtatg aatccgtctg
     6661 atgccattga ggcaattgaa aaaccgctct cctccctgcc ttactcactt tcccgtcaca
     6721 tcctggaaca tctgcgcaaa ctcaccagtc acgaacccgt gattggcatt atgtgtaaaa
     6781 gcggggccgg taaatcctca ctctgtaatg cactgtttca gggggaggtc accccggtca
     6841 gtgatgttca cgccggcacc cgggaagtgc agcgtttccg tctgagtggc catggtcaca
     6901 gcatggttat cactgacctg cccggggtgg gcgagagccg ggacagagat gcagagtatg
     6961 aagccctgta ccgtgacatt ctgcctgaac ttgacctggt actgtggctg attaaagccg
     7021 atgaccgtgc cctgtctgtg gatgagtatt tctggcgaca catcctgcac cggggacatc
     7081 agcaggtgct gtttgtggtg acgcaggccg acaaaacgga gccctgccat gaatgggata
     7141 tggccggtat tcagccttct cccgcacagg cacagaatat tcgcgaaaaa acggaggcgg
     7201 tattccgtct gttccggccc gtacatccgg ttgttgccgt atcggcccgc accggctggg
     7261 aactggatac gctggtcagt gcgctaatga cagcacttcc cgaccatgcc gccagtcccc
     7321 tgatgacccg cctgcaggac gagctgcgca cggaatctgt ccggggtcag gcccgtgaac
     7381 agtttaccgg tgcggtggac cggatatttg acacggcgga gagcgtctgt gttgcctctg
     7441 ttgcccgtac ggttctgcgt gccgtccgtg acacggtggt ctctgttgcc cgcgctgtat
     7501 ggaactggat tttcttctga acagtctgcc agggaaatgc ctgataacat tacgttaacc
     7561 aactgaaaac atgacacaaa cccctcatac tcctcctgtt tctcttgcgt gaccgccttg
     7621 tcgttaatag aataaaacta tcgataaaac agatatcgat cgttgctatc gatcgtttat
     7681 agtggttaat ggtctgcgaa aaggcag
//
LOCUS       DAJTNA010000179         7667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTNA010000179 DAJTNA010000000
VERSION     DAJTNA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.181"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..360)
                     /locus_tag="OO161_003387"
                     /pseudo
     CDS             complement(1..360)
                     /locus_tag="OO161_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(700..1254)
                     /locus_tag="OO161_003388"
                     /pseudo
     CDS             complement(700..1254)
                     /locus_tag="OO161_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1397..1519
                     /locus_tag="OO161_003389"
                     /pseudo
     CDS             1397..1519
                     /locus_tag="OO161_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="OO161_003390"
     CDS             complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="OO161_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5464342.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANIISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2629..2943)
                     /locus_tag="OO161_003391"
     CDS             complement(2629..2943)
                     /locus_tag="OO161_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464343.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3021..3131)
                     /locus_tag="OO161_003392"
                     /pseudo
     CDS             complement(3021..3131)
                     /locus_tag="OO161_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="OO161_003393"
     CDS             complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="OO161_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5464344.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4503..4640)
                     /locus_tag="OO161_003394"
                     /pseudo
     CDS             complement(4503..4640)
                     /locus_tag="OO161_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4722..5020)
                     /locus_tag="OO161_003395"
                     /pseudo
     CDS             complement(4722..5020)
                     /locus_tag="OO161_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5609..5768)
                     /locus_tag="OO161_003396"
                     /pseudo
     CDS             complement(5609..5768)
                     /locus_tag="OO161_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5772..6926)
                     /locus_tag="OO161_003397"
                     /pseudo
     CDS             complement(5772..6926)
                     /locus_tag="OO161_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6946..7293)
                     /gene="tnpB"
                     /locus_tag="OO161_003398"
     CDS             complement(6946..7293)
                     /gene="tnpB"
                     /locus_tag="OO161_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5464345.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7293..7667)
                     /locus_tag="OO161_003399"
                     /pseudo
     CDS             complement(7293..7667)
                     /locus_tag="OO161_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1978 a         1782 c         1676 g         2231 t
ORIGIN      
        1 tgttgttgcg ccttgctgcc aacgaagctc cactgcccat caagttcaca gataagggca
       61 acatcagcgt gagcgactgg cgcagatgtt atccgccggg gcgggagctt tttaatgtcc
      121 gtatgacggt attgatgccg attttcagcg tacgggcagt atcgcgaact ccggcaccgt
      181 tgaagccatt tcagtgattt ggtttttaac tcccggttta cgggcctcgt aacagtaagt
      241 gagctgaaac acccggtgac agtcacggca acggaatcga tcatgtcctt ttgggttctg
      301 cccgtgacgg taaacctgaa cggactggca acgggggcaa tgaaagttaa ctgtgaccat
      361 gaaagaacct taaaagcggg tattataacc cctaattcaa ctaattggag gcattgtcat
      421 ttttaatcta tggatacact taaaaatcct ctcaccttac gttaaaaacc ttctcatgtt
      481 tagagttttt tattatgttt atcataatga tatgacgatc tgagttttta aaatcaagaa
      541 gaaaagataa gttttttaaa tgcacagtgc ctgtggataa ctttgcgtaa atccggagga
      601 cagatcactc tgaagcaaga caacagcatc tgaaaacaat atgctcacgt ttcaaccaca
      661 gggcgtaaat gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc
      721 aaattgttcc ggactgaggc cgccacacca actgtgccgt cgccagcgat tgtaatcaca
      781 ttcgatataa ttaaacactg ttgcccgcat tatttcccgg ctgataaagt gttctccgtg
      841 gagacattcc actttcagcg aatgaaagaa gctttccacg caggcattat cgtagcagca
      901 gccctttgcg ctcatactgc cacgcagatt atgccacttc agcagcgcct gataatctgc
      961 tgaacagtac tgacttcctc ggtccgagtg aacgataacg ttccggggtc tcttacgccg
     1021 ccacagcgcc atctgcaggg catcgcaggc cagttgcgcc gtcaggcgtg gtgacatgga
     1081 ccagccaata acggcacgtg accacaggtc aatgaccaca gccagataca gccagccttc
     1141 atctgtacgt aagtacgtga tgtctcccgc ccacttctgg ttcggaccac tggcgtaaaa
     1201 atcctgctcc agcagatttt ctgacacagg caggccatgt gcgcggtaac tgaccgtatt
     1261 gctgggtaag atcaatttgc tcactggtat ttactacgtg catcgaatga gtgatcaaga
     1321 gccgtatgac gcgagagtgt cacgtacggt tccgtgagca gccggagagt gaaactctct
     1381 ccggctgact cgactcgcag gttccgacag cggtgacgaa catgcagcgg tgttgttctc
     1441 gctgatcggc acatgccgtc agaacaatgt ggagccagaa aaatggctgc gttacgttat
     1501 tgaacatatc caggactgac cggcaaaccg ggtacgcgat ctgttgcctt ggaaagttga
     1561 tctgacttct cagtaaatat caatatgatt ctgacgagcc gcttaccttt gagcagagaa
     1621 aggggagagg gatcccacat ccatgagcta ggaaaataca aaaactggct atacaacaat
     1681 tcaatatatt aggacttact tgcgaggaga aatatagcac cgctcaggca attcctgggt
     1741 atttactttt cttagtttct ttactcggga tgttctgtca tcagaaaggt gattctaaat
     1801 ttgtaatata gtcactcata ttcccacgta atttctttag atcaatatat tgcacatttt
     1861 tctcaaaaaa ctgatcggaa ataatatttg caacattgct tgttgtatca tttttaacgc
     1921 ttctattcat cccttcacaa agttcgtcca ttttcttttt gaaataactg cttcctgata
     1981 caagatgatt tatggaactt tgtgcagcat tgatgtctag agaaatgtga taatctttct
     2041 caacctgttt aaatatctgt ttacggagat tatcgccgat ttttcggctg tgttctgcac
     2101 aaaacttatc aatacattgg tttaccaccc gggatacgta gacttttttt tcatcatttt
     2161 tccaatctgt aatattttca ccgatttgtt ctgaaacaga gctcaaaata ctagtattta
     2221 ttttggttga tgatattttt actatccttt tccccattgt acagcggata atctcatcaa
     2281 cctttcctga aaaatatctt ttgtgagata aactgattcc aaaattatta aaagatgttc
     2341 caagcattat aatcactttg tcatgtaaaa tagtaacatt gtcattattg tagctgattc
     2401 aaacaattct gtaaatgctt tgagttcttt actcccttgg ctcaatgatg gaccgtctgg
     2461 aatcccagag actaaactct ctgagaaaga gagtatgaaa aaacttgggg cagtccctaa
     2521 atgaagggac caacagcggt aaaataaccg gcatcattca taatgatgcc ctgttagttg
     2581 tagttatgaa gatttacgtc attaaaattg ctgttcgtag tgtcagtcct atggtctggc
     2641 atcagctgag gatcgtagcc gatacatcac tggcttcacg acattcgtgt tgaggctatt
     2701 catgaaaact ccacgctgaa aacgccgttt tgtataaacg gtcacggtat gcccggagcc
     2761 actgttgctg atgaattcga taaaaccttg gcgtttcttg aagctatcgt taatgcggat
     2821 aatgaaacaa cgattggtga gattcgctct tttgccgaca ccctggacgc tgtccggttc
     2881 aatcgcaaca aaatcaaccg gcagctgagc aaacccaacc ttgcctctct ggcgctggag
     2941 catgaagtga tttagctggg acgtagccac tgacagcttc tataaaagta tgatcctacc
     3001 tacgtaatat gaacacaggc ttaagtgagg ttctggtttt caaattgttc aggactgaga
     3061 cctccacacc aactgtgccg tcgtcagcga ttgtaatcgc acttgatgta attaaacact
     3121 gtatatttca tttgtggagt ctccagtctg agggggcttt atgcattccg tattgggcac
     3181 tatgatgcca gaaatctgca aggctccaca ccttcctgta tttcccttcc cctatcacag
     3241 tgcactttac ttaatagggg ggaatgtgtt cattacattt attgtgtttt ttatgattgt
     3301 ttttgcgtcc agtgtcgttc gtcatgggaa actggttcta tgggtagtat cgctcagtct
     3361 agttaactgt ttgtttcttt ttagaaatta agcagatcac acaattcggg gagagatcct
     3421 tctaagtgca attctttgca gctatttagc acacttaaaa caactcttgt attttgtcag
     3481 aaataatatt aaacatgtct tgttcattat tgttaagtct gtctgctatt tctgtaagtt
     3541 ttatcccact atgatggcat atatttatat atgccatcat agcctcgata ttccctttcc
     3601 ctgcggccag aaataatcct ggtaacccct cattatctct tacatccaat agtttaatcg
     3661 tttcttttgg tgataaatca aaagttttaa taaaatctcc ataagcttga atcgcagcgg
     3721 aatgaccttc ttgaagagct atgaacaaag cagattcccc agcattattt cgtgctgcaa
     3781 gaacttctag tttttcctct ttgggtattt cggctgccaa cagcgattca ccgtatttac
     3841 gcgctgcatc ggcatttcca tgccaaatca atgaaaacaa attatggcgg ttatactcct
     3901 tattattctt tgtcggaatg gatatgctct gtattttggc caattttctg aaagaaacac
     3961 cactatatcc cccccctgat ttttcttcat tgatggcgtg ggatatagcg gtatttgtca
     4021 cgttaatgat attacgtttc attcctttat tttcattagc agtattgctg cctattatta
     4081 ggtgcatatt tttgttgttt gctggatgta atccatagtt atttattgac atttttgttc
     4141 atcctttttg ctaaaaaaaa caataatcta tttttgtaat gtaatagata ttagtctatt
     4201 gctaatctaa aaaaaccatt gccacattga atggctatat tcaccaataa attctttcga
     4261 tttcgtatat tgacatatcg tagatatatc ttatccctaa gattcaataa tcgagtccgt
     4321 ttaccattct gtgcaagcgc cttcactcag aagtgaccgt caaggcgatc accaaacttg
     4381 ataataaagc agctcattgc catacgccag tccttaaggg gcgttgtcca tttctgtgat
     4441 gctgcctaga tttaactgcc agctccggct tatctacatc taaatatgtg agcctaatac
     4501 cgctacactt tggccagact atgtttttct ccgggaagtg ggctaaccgt ctccataaag
     4561 tgacgaactt ttttcaacag tggccatatt gaacggcact gatgattacg ggttattgtc
     4621 tcatgaagag ccggccacat cactgacagc agtgacttca ggccttcagc accagacagc
     4681 ctgaaccagt taatccatcg cccgacagat ttagtaatct agggctggtt tttcttcagc
     4741 tgttgcggtt cgaaagtgcc attacgatca cgcagagtac ggatttccag aggttcatct
     4801 ccagtagtga taatcttcgt taagcaacca ttgcgggaat tgctgccaga tctaagctga
     4861 tttttctcgc acccaagatg atgagtcata ttagcattga gtacggtttc attttcagta
     4921 tccaatcaaa ctgactgaga tcttcaggat ttttgagact tttggctagt tcattagcta
     4981 aagcccgcaa cttattagat gtcaactgtt tttcattcat aaattcacct tcatttgata
     5041 tcaaattaaa tagttcaaca tcagagaatt atacaaactt atgtacaggc tcctatgatc
     5101 ttcgaatcct gaagattata gtgaaaataa gttttcacgt tgaaatccca catagttagt
     5161 atatgataaa taatcatgga tgacctgatt aacctcttgg ggtagtgctt ttatagaaga
     5221 ataaatattc tactaattta atacatcacc taaaatagta ttgtttttta tggttggttg
     5281 aaggtcgtgg tgatgatgtt agtccttttg agaatattcc agtgccagat cttacttata
     5341 ataaaggggc ttggcagttt ggataaacta ttgttcttat tccactaata caaacttttt
     5401 gtaaagtata ttaaatatac ttcatttcat actgaaatcc ccggaagtat tcaatcaaac
     5461 aattacacca taaaaggaag agggggccct aattaagttg tcaagcatgt ttatgatacc
     5521 tgcagggtat aaattttctg gctgcaagca gacacatata aaacgtcttt attgatccta
     5581 cccacataat atggacacag tcgtaagtga ggctctggtt ttcaaattgt tccggactga
     5641 gaccaccaca ccaacggtgc cgtcgccagc gattgtaatc gcactcgata taactaaaca
     5701 ctgttgcccg cactatttcc cgatggataa tagtgttctc cgtggagaca ttccattttc
     5761 agcgaatgaa ttttcatctg ttgctgtatc caatcataca gtgactgcat cagtggcgcg
     5821 gctctggctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5881 atggcataca gttcaccgat acgcagcagg gcttccgtta ttctgccgga ttcgtacaac
     5941 gcccggtaac caccgtaagc atcggcctga agcactccgc tgtaaccggc cagatgattc
     6001 tgtggatgga tacctttccg gtccggtgag tacgcgaacc agaccgccgg aggcatttcc
     6061 gaaccggcgt tacggtcatc acgaacgtag acccacagcc gggcagtcct tgttttaccg
     6121 ctgcccggct cctggaccgg aacggggata tcatcggcat ggactttacc gggcatcagc
     6181 acatactggc gcaggatgtc atacagcggt tccagcagtt cagcaacagc acctgcccag
     6241 cgccccagtg tggcacggct cagctccacg ccctgacgac ggtatatttc tgactggcgg
     6301 tataacggca gatagtctgc atattttcca gcgacaacat gggccagaag ccccgctccg
     6361 gcataactgc gtgcaatggg gtttgaaggc accggtgcct gcacgatatg gtcacaccgg
     6421 caacaggcca gtttcggacg ttgtgtttcg ataaccttaa aggcgctgct gataagctcc
     6481 agctgctctg acacatcaca tcccagagaa ctgagttcgc caccacaggc cgggcagcat
     6541 tcctcttccg gacggatgac ccgggtttca cggggaagtg aggcaggtaa cggtttacgg
     6601 gctgaagact ggcgcagggg agatggcagt accgggtcat attgctcacc cagcgtttcc
     6661 gccatttctt cctgaagtgc gctgattcgc tcctgtgctt ccagtatctg ccgttcggtt
     6721 tttgcgcgaa gtttttctga gcttttaccg aactgcatgc gctgcagttt cgcaaccagc
     6781 gccttcagcc ggttgatttc ggaagcataa gccgccaccc gctgtgagag caggcggttg
     6841 tatttcgcca tctggcggat ggtgtcctgt tgcgtctgca acagtgcccg caggcgggca
     6901 ttctcatgag caagtgaggt gtccatatcc tcactttaca acgggttata tgcggatgcc
     6961 agcgcgttcc gttcgtttcg ggtgtttcca gtcgatgccc tcaagaagca tggataactg
     7021 ggccggagta aggtgcacct tgccgtcacg ggtgaccggc cagacgaagc ggccccgctc
     7081 caggcgtctg gtaaagaggc acagtccgtc actgtcagcc cacaacactt ttatctggtc
     7141 accccggcgt ccgcggaaga tgaacagatg cccggagaac gggtcatcct tcaggacgtt
     7201 ctgaactttt gaggccaggc cgttaaagcc atttcgcata tcggtgatac ctgcaaccag
     7261 ccagatacgc gaacctgcag ggaaagatat catcagtggc tgctcccttt tatttcgcgg
     7321 ataagtatct gtaataacgc cggcgtcagt ttacctttaa gcctgagagt tccggccggc
     7381 agaaccagct cacagcacag actgtcggac ggtgtattta tctgctctgg ttcctgtgcc
     7441 ggagccggga ttttattatc cggctccggc gttaacgtca cgggaagcag tgcaggcata
     7501 tttttccgga aggcagcagg ccacctttcc ggtattgatg gcgccagttg aagagcaggt
     7561 tatcgttgat tccgttttca cgggcgatct gcgccacaca ggctccgggc tgcagtgact
     7621 gctccactaa ggcgatttta aattcataag gaaaattagg ccgccgg
//
LOCUS       DAJTNA010000180         7597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTNA010000180 DAJTNA010000000
VERSION     DAJTNA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.182"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..464)
                     /gene="bglG"
                     /locus_tag="OO161_003400"
     CDS             complement(<1..464)
                     /gene="bglG"
                     /locus_tag="OO161_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5464346.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVG"
     gene            complement(750..1475)
                     /gene="phoU"
                     /locus_tag="OO161_003401"
     CDS             complement(750..1475)
                     /gene="phoU"
                     /locus_tag="OO161_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5464347.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(1490..2263)
                     /gene="pstB"
                     /locus_tag="OO161_003402"
     CDS             complement(1490..2263)
                     /gene="pstB"
                     /locus_tag="OO161_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5464348.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2354..3244)
                     /gene="pstA"
                     /locus_tag="OO161_003403"
     CDS             complement(2354..3244)
                     /gene="pstA"
                     /locus_tag="OO161_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5464349.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(3244..4203)
                     /gene="pstC"
                     /locus_tag="OO161_003404"
     CDS             complement(3244..4203)
                     /gene="pstC"
                     /locus_tag="OO161_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5464350.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4290..5330)
                     /gene="pstS"
                     /locus_tag="OO161_003405"
     CDS             complement(4290..5330)
                     /gene="pstS"
                     /locus_tag="OO161_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5464351.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKYNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5578..6651)
                     /locus_tag="OO161_003406"
     CDS             complement(5578..6651)
                     /locus_tag="OO161_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5464352.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANEIKVGTTFNTTITNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYNLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(6662..>7597)
                     /locus_tag="OO161_003407"
     CDS             complement(6662..>7597)
                     /locus_tag="OO161_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5464353.1"
                     /translation="WKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLSG
                     WWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLTY
                     DGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLEI
                     DNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVKA
                     EFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQV
                     TLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
BASE COUNT         1963 a         1947 c         1735 g         1952 t
ORIGIN      
        1 cccacttcat ctttcggtaa ctgcacgccc aaccgtttat caataatggt taacgcttct
       61 tcccctagct ggaactcttt cgggtaaagc cgctggatat cccacagcaa cgggttgggc
      121 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
      181 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataat acgatcacag
      241 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
      301 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
      361 ttttgaaagc caattcctcg ccccatgacg actttttccc gctgttgatc atcaataacc
      421 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
      481 ataaacaaaa aaaaccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa
      541 tgcagtaaca atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat
      601 taatttaatt gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc
      661 gcagttatta actttgtgta attttaggaa tttataaagt tatatataac aaatcccaat
      721 aattaagtta ttgggatttg tctggtgaat tatttgtcgc tatcttttcc cgccagcagt
      781 ttatccagct catcaccgcc tacatgacgg aaatcctgcc ccttcacgta gtagaagata
      841 aactcacaaa tattctggca gcggtcgcca atacgttcga tagaacgcgc gcagaacagc
      901 gcagtaagta cgctcggaat ggtacgcgaa tcttccatca tgtaggtcat cagttgacga
      961 acaatacctt cgtattcctg atcgactttt ttatcttcac gataaatacg taccgcttcg
     1021 tcaatgtcca tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat ggtatgacgg
     1081 cccagcgact ccagacttac cagcaacggc tgatgctgct gggagaattt ctccagcgca
     1141 gtacggcaga ttttgtccgc cacgtcgcca atacgctcca gctcggcaat ggttttactg
     1201 atcaccataa ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat aatgcgcacg
     1261 caggcttcat cgatcgccac ttccatcatg ttgacgttct tgtcgccttc gatcacgcgc
     1321 ttcgccagat cgctgtcctg gttatgcatc gcggtgattg catcagaaag ctgctgctcc
     1381 accatgccgc ccatggtcat cacctgcgtg cggatacttt ccagttcggc gttgaactgg
     1441 ccggaaatat gtttattaag attgagactg tccataacgc actcctgaat caaccgtaac
     1501 gaccggtgat gtagtcttct gtttgtttct tcgctggctt ggtgaacaga tcgtccgtgt
     1561 tgctgaactc aatcaattcg cccaggtaca taaacgccgt gtggtcggaa caacgcgcag
     1621 cctgctgcat gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt tcggtgatca
     1681 gctcttcaat acgcccggta gagatagggt cgagcgccga acacggttcg tcgagcagca
     1741 gcacttccgg gcgaatggcg ataccacgcg caatacacag acgctgttgc tgaccaccag
     1801 agagagagta accgctctgg tgcaatttat ctttggtttc gttccacaat gcggctttgg
     1861 tcaatgccca ctgcacgcgc tcgtccatgt cggcacggga gagcttctca aacagacgaa
     1921 cgccaaaagc gatgttgtcg tagatggaca tcggaaacgg cgtcggtttc tggaacacca
     1981 tgcccacttt cgcacgcagc agtgcgatat cctgagagtt ggtcaggatg ttgtcgccat
     2041 caagcagaat ttcaccttcc gcacgctgct ccgggtacag ttcaaacatt ttgttgaagg
     2101 tacgcagcag cgtcgattta ccgcagccgg acggcccgat aaacgccgta acctggtttt
     2161 tagcgatatc caggttgatg tttttcaggg catggaattt gccgtagtag aagttcaaat
     2221 tacgaacctg aattttactc ggggcagttt caaccatact cattgcaatc ttttcctcat
     2281 cgtgcctgat gcacttcgct aatcaggctt accgtaggcc ggataaggcg ttcacgccgc
     2341 atccgacaaa gtgttaaccg tgtttattct tcgcaaaaac aacgcgcgct agaatgttca
     2401 gcagcagtac gcacagggta ataatcaata ccccggccca ggccagttgt tgccattccg
     2461 caaacgggct catcgcaaac ttaaagatcg tcaccggcag gttggcgatc ggctgcatca
     2521 tgtccgtgct ccagaactgg ttggagagcg cggtaaacag cagcggcgcg gtttcaccag
     2581 caatacgggc aatcgccagc aggataccgg tcatgatccc ggacaccgac gctttcagcg
     2641 taatcgcaga gatcatcttc cacttcggtg tacccagcgc ataagccgct tcacgcaggc
     2701 tgtccggcac cagtttcagc atgttctcgg tggtgcggat aacaatcggc acctgcaaca
     2761 gcgccagggc aatcacgccc gcccagccag agaagtgctc catctgcgcc accacgatgg
     2821 tgtaaacaaa cagaccaacc acaatcgacg gcgcggagag cagaatgtcg ttaatgaagc
     2881 gaatcacttc tgccagccag gatttacgac catattccgc cagataaatc cccgccataa
     2941 tgcccagcgg cgtaccgaat accgtggccc acaaaattaa cagcccgcta cccgccaggg
     3001 cgttcgctag accaccacct tccgtattgg gcggcggcgt catttcagtg aacagcgcca
     3061 gcgacatacc gtcgataccg cgagtgatgg tggacattaa aatccagatc agccagaaca
     3121 ggccgaaggc catcgtcgcc atcgagagcg tcagcgcaat acggtttttg aggcggcgac
     3181 gcgcctgcat tttgcggcga gattcagcca gcgccgcagt ggtttggatt tcaaccatag
     3241 ccattagcgt gccccctcat tcttagccag gcgcataatc ataaacttcg atgcggcgag
     3301 gacgatgaag gtaatcacaa acaggatcag gcccagttcc atcagtgcgg caacgtgcag
     3361 accggattcc gcttccgcaa attcgttcgc caacgcagag gtgatactat tgcccggcat
     3421 gtacagcgag gcgctgtcga gctggtaggt gttaccgatg ataaaggtca ccgccatggt
     3481 ttcaccgagc gcgcggccca gccccagcat gatgccgccg ataacaccat ttttggtgaa
     3541 cggaagaacg atacgccaga taacttccca ggtggtgcag ccaataccgt aggccgactc
     3601 tttcatcatc accggggttt gttcgaacac atcacgcatt accgccgcaa tgtacggaat
     3661 aatcatgatg gcgaggatca cgcctgccgc gaggataccg ataccaaatg cggggccaga
     3721 gaacagcgcg ccaacaatcg ggatattcga catgatattg ccgaccggct cctgaaagta
     3781 aacggcgaac agcggcgcaa agataaacag gccccacatg ccgtaaacga tacttggaat
     3841 ggctgccagc agctcaatgg cgatacccag cgggcgtttc agccagccag gcgcaagctc
     3901 agtcaggaac agggcgatac cgaaactcac cgggacggcg atcagcagcg cgataaacga
     3961 agtcaccaac gtaccgtaga tcggcaccag cgccccgtag atatcgttcg gtgcatccca
     4021 ctctttggtc cataggaaag ccagaccaaa tttctgaatg ctcggccagg aggagatgat
     4081 cagagagaca ataatgccac ccaacatcaa tagcacaatc agcgccgcca gttttaccag
     4141 cacgctgaaa attatgtcgc cctttttacc cggtgggtta aaagcaggct tggttgcagc
     4201 cataagttac tcttcagtta aacgcgttta caaagtcatt ttgtaatgcc ggatgcggcg
     4261 taaaacaccg tacccggcct ggagttttat tagtacagcg gcttaccgct actgtcttta
     4321 atattggtct tccacgcagc gcgaacctgt tcaactacac tatccggcag gctggcgtaa
     4381 tccaggtcgt tcgcctgttt agccccggtt ttgtacgccc agtcgaagaa tttcagcact
     4441 tctgtgcctt gttctggttt cttctgatct ttgtggatca gaatgaacgt ggtagaggta
     4501 ataggccatg catcttcgcc tttctggttg gtcagatcct gagcgaaggt tttgctccag
     4561 tctgcacctt ttgctgcatt agcgaagttt tcttcggtcg gactaaccgg tttaccatca
     4621 gcggagatca gtttggtgta cgccaggttg ttctgcttcg cgtaagcata ttcaacataa
     4681 ccaattgcac ccggcagacg ctgaacgaac gcggcgatac cgtcgttacc tttaccgccc
     4741 agaccgatcg gccattttac ggtagagcca gtaccaacgt tgtatttcca ctcttcgttc
     4801 actttcgcca ggtagctggt gaagacgaag gaagtcccgg agccatctgc gcggcgtact
     4861 acagcaatgt tttgtgaagg cagtttcaga cccggattca gtttggcgat ggcttcatca
     4921 tcccacttct tgattttgcc caggtagatg tcgccgaggg ttttaccatc cagcaccagt
     4981 tcgccagact tcagccctgg aatgttaacc gccagcacca cgccgccaat cacggtcggg
     5041 aactggaaca gaccttcctg agccagtttt tcgtcagaca gcggcgcgtc agaggcacca
     5101 aaatcaacgg tattagcgat aatctgtttt acgccaccgg aagaaccgat accctggtag
     5161 ttaactttat taccggtttc tttctggtaa gtgtcagccc atttggcata caccggcgca
     5221 gggaaggttg cacctgcacc tgtcaggctt gcttctgcaa acacagagaa agcgctcatc
     5281 gataaggtcg cggcgacaac agttgcgacg gtggtacgca taactttcat aatgtctcct
     5341 gggaggattc ataaagcatt gtttgttggc tacgagaagc aaaataggac aaacaggtga
     5401 cagttatatg taaggaatat gacattttta tgacagaaag gaattaccaa ataacttact
     5461 gattttaaat gtgaagatga aaatcccaca tgtcatcttt ttgtcatgat gcatttaaaa
     5521 gtgataaaga aatgggtgag atgacaacga tgtgatgaac aatcgttgtc agacctatta
     5581 gcgcacaatc tcaacagtta tcgtcgctgt tgcagtaaat tgccccgggg ctgggcgagc
     5641 tccagtaagg ctcgcaggag cagctgaaaa agttacacta cctgattttt caccatacac
     5701 tgttgattta cccatgtctg ttggcaactc accaccgttc actttgattt ccttattatt
     5761 tttgtcgtaa atttttatcc ccacgttaga gtttgaagtt acgaccattg aattgtccgt
     5821 attgggttcg gtagcataaa aagacgtact gatatcatcg gtattcagta tccccaggtt
     5881 ttcacactca atagtgaccg ttttagtaat tttatgtgtt gttaccgcac ttccaacaac
     5941 agatgaaaaa tctgcagctg gtatttcttt aaaatcaaaa taaactggct gtaaattatt
     6001 tatttcgcaa ctttgcggag ctgtaatatc gccccgaata taaacatcag ccatcggctg
     6061 tgagtaatcc acaggggtgt cacggctaat tgttccatac aatgtcacta tctttgtttc
     6121 aggaataatc aattttcctg caaatgtctt cttgatataa aaatatacta tcgctttagc
     6181 cccactagat atacttaaag gttcaatgcg gttgcaattg tactttgtat taccagacgg
     6241 gttgttagca tgatattcaa aaggtgcatt cacataccca accccaagaa tatctatagc
     6301 aatcccaaca tcaaggttcg gattaagatt ataataatta atattagagt gaaccccagc
     6361 ataagttaat gcctggttta tttttgacgt ataatagata taggggaatt ctcctacgtt
     6421 accagtatca caatgacagc gtccaggata tgaggatcca ttagttatag tggtgttaaa
     6481 tgtcgttcca accttaattt cgttcgcggt aataagatat cccgagaaat caagcgtact
     6541 gtgaaatacg ccattatcag gggtacagat acctttagga gctgcaagtg ctacactact
     6601 taacgagagc ataaacacag caaagcacca ctgtttgata tacttgttca taaaaatcct
     6661 tttaatgaca tgtcacaccc tgttcaatta atccgctctc actttctggt gtcgaaagtg
     6721 aataattgaa gatgcatttt gttgaggcgt tttctcccca ggataacgta acctgtcctt
     6781 tttctggtag accagaaaga taaagttcgc cacggtcacc gacaattcct gaatctgcca
     6841 aacttgcatc ttgtatcgca gcaatggctc caaaaggtac cggtttacca tttgcattta
     6901 acactctgaa gagaacgcga taacccacat gagtcacaaa ctccgcttta accactgcac
     6961 cacgcgtggg aaccatcgta acgacggtat tatcaagttc tatattcgac ttcgagaaat
     7021 agttatcgct gattgctacc cgattttcat cataagggtt tagctgtgtc ttgaccgtat
     7081 agccgcgcca gtccgtcgca gcaccgcgca tattatctat ttctaacccg gcggccccag
     7141 gtgctttaac aagagcaata gtttcaccta actcctgact taacgttacc ccttcgctgt
     7201 gtgccagaat tccccctctg ataccataat tcagcctctg ggaattttgg ctgtagttat
     7261 agctgccgtt caccgtgcca taagagccat cataggtcag ggacatatta ccgccaacct
     7321 catcatcatg actgttatag gactgcatta aattccatga aaggttattt ctttccagcg
     7381 ccgtaccgct gagtccgagt tgatgtgagc taccggaatt atcgttttgc gaaacagaat
     7441 aggtggcgta actattaccc caccagcccg ataaaggaac agataaagag agcatgtaga
     7501 tctgctcact ggtgttatca ctggcatggt tgttccccgt cccctgtctg gcagtattgg
     7561 aataactcca ggagacgttc cacgagagac gcttcca
//
LOCUS       DAJTNA010000181         7590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTNA010000181 DAJTNA010000000
VERSION     DAJTNA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.183"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..1226
                     /locus_tag="OO161_003408"
     CDS             <1..1226
                     /locus_tag="OO161_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5464354.1"
                     /translation="INMMSRLEHVEEEKINYDFFLNLPEINRSKLERIDIRTSQLITP
                     LFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLPSTPYTTDANGRGP
                     AWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLTALKSDATPEVRRE
                     QVAALRQQLNDVTEAHELLRDADALVEKSIWLIGGDGWAYDIGFGGLDHVLSLTENVN
                     ILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMMMYGHVYVAQISLG
                     AQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQLTATGFWPLYRFDP
                     RRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQLWKDAAADLQKRYD
                     FLAQMAGKAEKSNTD"
     gene            1592..2725
                     /gene="ompN"
                     /locus_tag="OO161_003409"
     CDS             1592..2725
                     /gene="ompN"
                     /locus_tag="OO161_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR5464355.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            2866..3300
                     /gene="uspF"
                     /locus_tag="OO161_003410"
     CDS             2866..3300
                     /gene="uspF"
                     /locus_tag="OO161_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5464356.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKQIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            3857..4771
                     /gene="sitA"
                     /locus_tag="OO161_003411"
     CDS             3857..4771
                     /gene="sitA"
                     /locus_tag="OO161_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5464357.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            4771..5598
                     /gene="sitB"
                     /locus_tag="OO161_003412"
     CDS             4771..5598
                     /gene="sitB"
                     /locus_tag="OO161_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5464358.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            5595..6452
                     /gene="sitC"
                     /locus_tag="OO161_003413"
     CDS             5595..6452
                     /gene="sitC"
                     /locus_tag="OO161_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5464359.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRL"
     gene            6449..7306
                     /gene="sitD"
                     /locus_tag="OO161_003414"
     CDS             6449..7306
                     /gene="sitD"
                     /locus_tag="OO161_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5464360.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         1949 a         1793 c         1820 g         2028 t
ORIGIN      
        1 ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga gaaaatcaat tacgatttct
       61 tcctcaacct gccagaaatc aaccgtagca aactggaacg tattgatatt cgtacatcgc
      121 agctgattac accgctgttt gaatattccg gtgcttgctc cggttgtggc gagacgccgt
      181 atattaaatt gctgactcag ctctatggcg accggatgtt gatcgctaac gccaccggct
      241 gttcttcaat ttatggcggt aacctgccct ctacaccgta taccaccgat gccaacggtc
      301 gtgggccggc atgggcgaac tctctatttg aagataatgc cgaatttggc cttggtttcc
      361 gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct gctggatcaa tttgccgata
      421 aaatcccggc ggaattactg acggcgttga aatcagacgc tacgccagag gttcgtcgag
      481 aacaggtcgc agctttacgc cagcaactca acgatgttac cgaagcacat gaactgctac
      541 gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat tggtggtgat ggctgggctt
      601 acgatatcgg ctttggcggt ctggatcatg tattgagttt gacggaaaac gtcaacattc
      661 tggtgctgga tacgcaatgc tattccaaca ccggtggtca ggcgtcgaaa gcgacaccgc
      721 tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa agcgcgtaaa gatcttggcg
      781 tcagtatgat gatgtacggt catgtttatg tggcgcagat ttctctcggc gcgcagctga
      841 accagacggt gaaagcgatt caggaagcgg aagcgtatcc gggaccatcg ctgatcattg
      901 cttatagccc gtgtgaagag catggttacg atctggcact cagccatgac cagatgcgcc
      961 aactcacagc taccggcttc tggccgctat atcgcttcga tcctcgtcgt gccgatgaag
     1021 gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga agcactggaa gaaacgttac
     1081 ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc agaagtggca gaacagttat
     1141 ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt cctggcacaa atggccggaa
     1201 aagcggaaaa aagcaacacc gattaaatgc tctggataag gattatccaa ttctaaaaaa
     1261 aagcccggac gactgttcgg gcttgtcttt ttatatgttg gaaaatcagt ggcaatgcaa
     1321 tggcccaaca gaaaataaaa tctgtgacaa taaaggcata taacccgcgc agaataacgt
     1381 atagaaaatt aaaagtataa tttttatttt gtatgtatta tcttcgatac attcaccaga
     1441 cttattatca tttcagaaga attatttgat taaggtttta cttaaggcgt aacaaatgat
     1501 atttatcggc taactgaact tctcctttag gatgttttca ctccccggaa tggggataaa
     1561 aggcaaataa aataactaaa ggatttattc aatgaaaagc aaagtactgg cacttttaat
     1621 tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa gtttataata aagacggcaa
     1681 caaattagat ctgtatggca aagttgatgg cctgcattat ttttctgata attcagcgaa
     1741 agatggcgat cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca
     1801 actcactggc tacggtcaat gggaatacaa tattcaggca aacaacactg aatcttcaaa
     1861 aaaccagtca tggacccgtc tggcatttgc cgggctgaaa tttgcagatt acggttcttt
     1921 cgattacgga cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc
     1981 tgaatttggc ggtgactctt ataccaatgc agacaacttt atgactggtc gagccaatgg
     2041 cgtcgcgact tatcgtaata ctgatttctt cggtctggta aatggtctga acttcgcggt
     2101 gcagtatcaa ggtaacaacg aaggagccag taatggtcag gaaggcacca acaacggacg
     2161 tgatgttcgc catgaaaacg gtgacggctg gggtttttcc acaacatatg atttaggcat
     2221 gggctttagc gctggtgcgg catacacctc ttctgaccgc accaatgacc aggttaacca
     2281 tactgcggcg ggtggtgata aagcagacgc gtggactgct gggctaaaat acgatgctaa
     2341 caatatttac ctggcaacca tgtattcaga aacgcgtaat atgaccccgt ttggcgacag
     2401 cgattatgct gtggcaaaca aaacccagaa ttttgaagtc actgcccagt accagtttga
     2461 ttttggtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc tgcacgctgc
     2521 cggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat acgccgatgt
     2581 tggcgcgact tactatttca ataaaaacat gtccacctac gttgactata aaatcaacct
     2641 gttggatgaa gatgacagct tctacgctgc caatggcatc tctaccgatg atattgtcgc
     2701 tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg gctttttctg
     2761 cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccatcattac acaaaaagtg
     2821 tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa cagaacgatt
     2881 cttgtcccta tcgatatttc cgattcagaa ttaactcaac gcgtgattag ccacgttgag
     2941 gcagaggcaa agattgacga tgcagaggtt catttcctga cggtaatacc ttcactgccc
     3001 tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga tgacctgaaa
     3061 gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc aaccgacaga
     3121 gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt ggcgaagcag
     3181 atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac ttatctgctc
     3241 ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga
     3301 cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt
     3361 tcccctctaa tccgtaccat acacgccact gtttttatat cagatttctt atgctgggca
     3421 ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct tttaaatgtc
     3481 gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaagtaag ggcaaaaatc
     3541 acaactatct gactcataag tattttattt atttttcaat atgttaaaca ttatcagcga
     3601 caacaataag ctacgataac ccactctttt tttgccccat ttttgccatc aacaattttc
     3661 ctcataaagt catttcaaag cctaatagct gctctacttt tttcctcaat atcatgtcgc
     3721 cagcaggaaa caacctaata cctgttttag gcctcaaagc tgttagtgat aattattttc
     3781 attttgtata ggttatgcta taaaacatag ccgttgctat gtttgttcta attttgctca
     3841 ctataggtac taaattatgc actcaataaa aaaagtaacc atgctcttgg ggtgcctcgc
     3901 cctcacctgc tcgatcgcat ttcaggcaag tgcaactgaa aaattcaagg tcattacaac
     3961 atttaccatc atcgcggata tggccaaaaa cgtggctgga gatgctgcag aagtctcatc
     4021 cataaccaag cctggtgcag aaattcatga gtatcagcct acccctggcg atattaaacg
     4081 tgcacagggg gcacaactga ttctcgccaa tggtatgaat ctggaattgt ggttccaacg
     4141 cttttaccag catctcaatg gggttccaga agtaattgtc tcttcgggtg tgacgccagt
     4201 agggatcacc gaaggaccct atgagggcaa acctaatccc catgcatgga tgtcgccaga
     4261 taatgctctg atttacgtcg ataatattcg tgatgcgttg ataaaatacg atccggcaaa
     4321 tgcacaaacc taccaacgta atgccgatac ttataaagcc aagattaccc aaacccttgc
     4381 ccccctgcgt aagcagatta cggaactccc tgagaatcag cgatggatgg tcaccagtga
     4441 aggggctttt tcttatctcg cacgcgattt ggggctaaaa gagctttatc tgtggccgat
     4501 taatgccgat caacaaggaa caccgcagca ggtacgtaag gttgttgata tagttaagaa
     4561 aaatcatatc ccggcagtct ttagcgagag tacgatttcc gataaaccag cgcgtcaggt
     4621 tgcgcgtgaa accggcgcac actacggcgg tgtgctctat gtcgattccc tgagcacaga
     4681 aaacggcccg gtacctacgt atatcgacct tcttaaggtt actaccagta cgctggtaca
     4741 gggaattaaa gccgggaaaa gggagaaata atgatgcaat ctgcaggcat tgtcgttaat
     4801 gatgttactg tcacctggcg taacgggcac acagcactgc gcgatgcatc cttcacagta
     4861 ccaagtggat ctattgccgc tctggttggg gtaaacggtt ccggaaaatc gacgttgttt
     4921 aaagcgatta tgggatttgt gcgtctgacc agcggaaaga tatctgttct gggtattccc
     4981 acgcgacagg cgctacagaa aaacctggtt gcctacgtcc ctcagtcaga agaggttgac
     5041 tggtcatttc ctgtactggt ggaagatgtg gtgatgatgg gccgctatgg gtatatgggt
     5101 atgttgcgta tagccaaaaa gagggatcgt cagattgtca ccgatgcgct ggaacgtgtc
     5161 gatatggtgg attttcgcca tcgtcaaatc ggcgagcttt ccggtggaca aaagaaacgc
     5221 gtctttctgg cgagagcgat tgcacagcag ggtgatgtga tcctgctgga tgaacctttt
     5281 accggcgttg atgtgaaaac cgaagcgaaa atcatcagcc tgttgaggga gttacgcgcg
     5341 gaaggcaaaa cgatgcttgt ctcaacgcac aatctcggct cagtcacgac attttgcgat
     5401 tatacggtca tggtcaaagg caccgtcctg gcaagcgggc cgacagacac cacttttaca
     5461 gccgagaacc tggagctggc ttttagcggc gtactgcgcc atgtcacact taacggctcg
     5521 gaagaaagca ttattactga cgatgaacgc ccttttgtgg cacatcgacc gtcagcggtg
     5581 cagagggaag caagatgaac gtgctactgg aacccttcag ctatgaatat atgctcaatg
     5641 cgatgtgggt ctcggcaatg gtcggcggtc tctgcgcttt cctgtcatgc tatctgatgc
     5701 taaaaggctg gtcgctgatt ggcgatgcgc tgtcgcactc tattgtcccg ggtgttgcgg
     5761 gggcatatat gctggggctt ccgttttctc tgggagcctt cttttccggc ggactggcag
     5821 cgggcagcat gctgtttctt aaccaacgca cccgcttaaa agaagatgcc atcatcggcc
     5881 tgatcttctc gtcctttttt ggcctggggc tgtttatggt gtcattgaac ccgacatccg
     5941 tgaacattca gaccatcgtc ctgggcaata ttctggcaat cgatccggcg gatatcctgc
     6001 aactcactat cattggcatt ctctcgatca tcgtgctttt tttcaaatgg aaagatctta
     6061 tggtgacctt tttcgacgag aatcacgccc gtgctattgg attacatccg ggcagattaa
     6121 aaatcctctt cttcaccctg ctgtcggttt cgaccgttgc ggcgctgcaa acggttggtg
     6181 cctttctggt gatctgtctg gtggtgactc ccggtgcaac agcatggttg ttaaccgatc
     6241 gttttccccg cctgctcata attgctgtaa ctattggcag tgtaaccagt ttcctcggcg
     6301 cgtgggtcag ttactttctg gacggcgcga caggtggaat catcgtggtg gcgcagacac
     6361 tgctgtttct tttagcattt gtatttgcgc ccacccatgg acttttggca aatcgtcggc
     6421 gtgcgcataa agcgctggag gatcgcttat gatggcatta ttactggagc cactgcagtt
     6481 cacctttatg agtcacgcac tgctgatttc actggtagtt tccataccct gtgcgctgct
     6541 gtcggtcttt ctggtcctga aaggttgggc actgatgggt gacgcgatga gtcatgcggt
     6601 ttttcctggc atcgtgctta cctggattct ggggttacct ctggctacag gggcattcgt
     6661 cgccggagta ttctgcgcgg tggcgacggg atacctgaaa gacaacagcc gaatcaagca
     6721 agatacagtt atggggattg ttttttctgg catgtttgcc gcaggtctga tcttgtatat
     6781 tgcaatcaaa ccagacgtac atcttgacca tattcttttc ggcgacatgc tcgggataac
     6841 catcggcgat ataatccaga cggtgattat tgccgggctg gttacacttg ttattagcgt
     6901 aaaatggcgg gattttttgc tgttcagctt tgattatcaa caggcgcagg taagcggttt
     6961 gcatacgaga tggctgcatt atgggctgct gtgcatggtt tccctgacca ttgtggcgac
     7021 gctgaaagca gtaggtatca ttctctctat ttctctgctc attgcgcctg gcgccattgc
     7081 agtactcctg acacaacgct ttcatattgc gctcctactg gcgactggca tatcggtaat
     7141 agtgtcaatg actggtgtct ggctgtcctt ttttatcgac agcgcaccag ctccgacgat
     7201 tgtcgtctta ttcgcggtcg tgttcatcat gacgtttacc gttaccagca tcaacgcacg
     7261 cacaaaggga aacgcccata cacaggatct gttatcaccc aattagcaca atatccgagg
     7321 acagcatcgt ccgccagcct acagaagtcc ggaaagccat ggaaggtcgt tgggaggagg
     7381 tacgaattct atcatgcaaa aaatacgtaa aatcgataac gcctagaaat cattcaatac
     7441 tcgcactatc gaaagtttac cagctaaccg cggcacgttc ctgcatacga cgtgccgcag
     7501 cttctgttat tccacgataa aattcctaac cgaattcaaa tccttcagac tattcagctc
     7561 ttccttcagc tcccgctgac ggcgataaat
//
LOCUS       DAJTNA010000182         7573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTNA010000182 DAJTNA010000000
VERSION     DAJTNA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.184"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            4..420
                     /locus_tag="OO161_003415"
     CDS             4..420
                     /locus_tag="OO161_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5464361.1"
                     /translation="MPPFFTEPERNMAKKDENLKRLKELSELLGREPDMSGSAADIAQ
                     RVAELEEELGGVDGTDNQASVLTGQENNGEAVPPERMIQNTDELVRVVARTTLHIDAL
                     HATQDKPVKLVLPETVFRVPAGMATSLVKDDLVKMQ"
     gene            432..800
                     /locus_tag="OO161_003416"
     CDS             432..800
                     /locus_tag="OO161_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5464362.1"
                     /translation="MADFDNLFDAALDLADRAIIRNMGITAVITSGQLKGHRISGVFD
                     DPESISLVAGGVRIEDSLPSLFVKTADILQLCRNDSLMIGRESFSVDRITPDDGGCSY
                     IRLRREGAGNVRAGGYYEGA"
     gene            787..1374
                     /locus_tag="OO161_003417"
     CDS             787..1374
                     /locus_tag="OO161_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5464363.1"
                     /translation="MRVLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            1371..1595
                     /locus_tag="OO161_003418"
     CDS             1371..1595
                     /locus_tag="OO161_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464364.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVRLRSYCATTAC"
     gene            1774..2460
                     /locus_tag="OO161_003419"
     CDS             1774..2460
                     /locus_tag="OO161_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5464365.1"
                     /translation="MYTGKGDAFANPLSDTDWLRLAMVKDLQPGEMTADAEDDTYLDD
                     EDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDSGEVCAFRIKYPNGTVDVF
                     RGWMSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVGVTGLTVAPASVSVKAGAT
                     TTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKGVKQGSVSIVGMTSDGEFV
                     AVAAVTVSAP"
     gene            2519..2905
                     /locus_tag="OO161_003420"
     CDS             2519..2905
                     /locus_tag="OO161_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5464366.1"
                     /translation="MFLKQDTFNYEKQSVVLSELYGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLLSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2914..3242
                     /locus_tag="OO161_003421"
                     /pseudo
     CDS             2914..3242
                     /locus_tag="OO161_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            3214..6255
                     /locus_tag="OO161_003422"
     CDS             3214..6255
                     /locus_tag="OO161_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5464367.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAGEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     VWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAVLNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            6255..6584
                     /locus_tag="OO161_003423"
     CDS             6255..6584
                     /locus_tag="OO161_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5464368.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6584..7282
                     /locus_tag="OO161_003424"
     CDS             6584..7282
                     /locus_tag="OO161_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5464369.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVAGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     VVFPGHIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            7287..>7573
                     /locus_tag="OO161_003425"
     CDS             7287..>7573
                     /locus_tag="OO161_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCR5464370.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLV"
BASE COUNT         1810 a         1732 c         2460 g         1571 t
ORIGIN      
        1 agtatgcccc cgttttttac tgaaccggaa agaaatatgg caaagaaaga cgagaatctg
       61 aaacgtctga aagagctgtc agaattgctg gggcgtgagc ctgatatgtc gggaagcgcg
      121 gcagatatcg cacagcgtgt ggcagagctg gaagaggagc ttggtggtgt ggatggcact
      181 gacaaccagg caagtgtgct taccgggcag gaaaataacg gggaggcagt tccgccggag
      241 aggatgattc agaatacgga tgagctggtc agggtggtgg cacggacgac gctgcatatt
      301 gacgcgcttc acgccacaca ggataagcca gtgaaactgg ttctgccgga aacggtgttt
      361 cgcgtgcctg ccggcatggc caccagtctg gtaaaagatg acctggtaaa aatgcagtag
      421 caggggggat tgtggctgat ttcgataatc tgtttgatgc cgcgcttgat ctcgcagaca
      481 gggccattat tcgcaatatg ggcattactg cagtcattac atcaggccag ttaaaaggac
      541 accggatttc cggcgttttt gacgatcctg aaagtatttc tctggtagcc ggcggtgtgc
      601 gtattgaaga ctctttacca tccctgtttg tgaaaacggc agatatttta cagctgtgtc
      661 gcaatgattc gctgatgatt ggccgggagt ctttcagcgt ggatcgtatt actcctgatg
      721 acggcggatg tagttatatc cggttgcggc gtgaaggagc aggaaacgta agggcagggg
      781 gatattatga gggtgcttga gaatgccatc cgcaatctga acagtcttga tacccgtatg
      841 gtgccgcagg ccagcgcatg ggcagtaaac cgggttgctg caaaagttgt ctcggttgcc
      901 acccggcagg tcgccaggaa taccgttgcg ggagataacc aggtgaaggg gggccccctg
      961 aaactggtgc gtcagcgcgt ccgggtattt aaagccagtc cggcaggaaa aatgtatgcc
     1021 cgtatccgtg ttaaccgggg caatctgccc gccattaagc ttggcacaac atgccggcgt
     1081 gccggtgacg gactgaaggt ggggaaatac ttttttcgtg gtgcgtttat ccagcagctg
     1141 gcgaacggtc gctggcatgt tctcaggcgt cttcctgaag cgtgttttga aacaggaaag
     1201 gacagtaagg ggcggcccag aaaaaatcgt ctgcccgtgg aggttgtgaa aattccgctg
     1261 gccggaccgc tgacacaggc atttgaggat gcccgcgacc gcatcattgc cgcggaaatg
     1321 ccgaaacagc tggggtatgc cctgaaacag caactgagga tacatttggg atgaacaggc
     1381 acacacaaat ccgccaggca gtactggcaa gcctgaaggg cgaatgcggg gggaatgtcg
     1441 tgctttttga cgggcttccg gcatttattg atgcgcagga actgcccgcc gtggcggtgt
     1501 ggctgagtga tgcgcagtac accggaaaaa tgacggatga ggacgactgg caggctgtac
     1561 gcttacgttc atattgcgcc accactgcat gctgatgtac cacagcccgt cacgaatgag
     1621 ccagtcacgc tcttccttcc cggtggagta cggatgtgct gtcagccgtc acaacttacc
     1681 gatgtcttca gggtaagcgt caatacggtg ctccgttgac gcttaccacc aaatccactg
     1741 gcaaagacga aaggtgcggg aacgacgttc tggatgtaca ccggcaaggg cgatgcgttt
     1801 gcgaaccctt tgtcggacac tgactggctg cgtcttgcga tggtgaagga tctgcaacct
     1861 ggcgaaatga ccgctgatgc agaagatgac acttatctcg atgatgaaga tgcagactgg
     1921 aaaacgacaa cccaggggca gaaatccgtc ggtgatactt tggcgacgct ggcctggcgt
     1981 ccgggtgaca gcgggcagaa aaaactggtt cagttgttcg attccggtga agtctgcgcg
     2041 tttcgtatca aataccccaa cggtacggtt gatgttttcc gtggctggat gagttcactg
     2101 ggtaaaacca ttacctcaaa agaggtgatg acccgtactg taaaaatcag cggtgtgggg
     2161 cgtccgtatc tggcagagga gggcgctgaa accgtgggtg ttaccggact gacggtggca
     2221 ccggcatctg tcagtgtcaa agcgggagca accaccacac tgacctttac agtaaaacct
     2281 gatggggcca gtgacaaagc gatcagtgtg cattcgtcag atcctcagac tgcttcggtg
     2341 accctgagcg ggcttgtggc cacggtgaaa ggcgtgaagc agggcagtgt cagcatcgtg
     2401 ggcatgacct ctgacggaga gtttgtggca gtggctgcgg tgaccgtcag cgcaccataa
     2461 caggacgata ctcatcattg ccccggttat ccggggcttt ttgcatccgg agaacatgat
     2521 gtttctgaaa caggatacgt ttaattatga aaaacagtcc gtggtgctca gtgagctgta
     2581 cgggctgcag agaattgaat atctgacgtt tgttcagcag cgaacggcaa agtttgatgc
     2641 acaggaggga gaactgccgg aggctgaacg acagattgct tttctgcgta tgggaatgga
     2701 tatcaatgcc tggctgcttt cccgctcact gtggaatgct gagcagtctc aggatgttga
     2761 gacgctttgc gcatccatta tgacaacatg gtcgtatgat gcgctgggcg cgggggcgga
     2821 gagggttctg tcgctgagcg gtatggggac cattgagaat gccggggatg atgatcatga
     2881 ggcgctgacg ccggaaaagt cctgacgcgg gaaatgcagt ttgtcatgcg gcttgcccgg
     2941 gagttccggc gggcagactg gcggcggatg ctgtcggaaa tgtcggccac tgagcttggt
     3001 gagggggcga ttatttccgg atgcagagct tcagtgatgt gtggatggat gcgcagtttg
     3061 cctcgctgaa ggcattgatc gtgagaatgg tgtccggcag cagtgatgct gcggtggctg
     3121 atttcagcct tttaccggaa gagaacggga taccggagcg aacggacgaa gaactgatgc
     3181 atcttgggga aggtatttcc ggaggtgtgc gttatggacc agatagccaa cctggtcatt
     3241 gatttgggga ttgatgcggc agagtttaaa aatgaaattc cccgtatcaa aaaccttctg
     3301 aatggtgcag ccggtgatgc agaacggtct tctgcccgta tgcagcgttt tatggagcgt
     3361 cagactcagg cggcccggca gacaatgcat gcggcttctt cggctgcaac agcagcatca
     3421 gcccatgcgc agacggtgga gaagaatgca cgggctcatg aacgcatggc ccgtgaggtg
     3481 gaacaaaccc gtctgcgcgt ggatgccctg aatcagaaaa tgcgcgagga acaggcgcag
     3541 gccagggcac tggcggaggc gcaggataaa gcggctgccg ccttttatcg ccagattgac
     3601 agtgtgaaac aggccggtgc ggggcttcag gaattacagc gtattcagca gcagatccga
     3661 caggccagaa acagtggcgg ggttggtcag caggattatc tggcgctgat ttcggagatc
     3721 acggcgaaaa cccgtgccct gacgcaggca gaggaacagg ccacccggca gaaagcggcg
     3781 tttatccgcc agcttaaaga gcaggcaacc cgccagaatc tgtcgtcttc tgagttgctt
     3841 cgcgccaggg cggctcagct gggggtaagc agtgctggag aagtgtatat ccgcaaaatg
     3901 gagcgggcag gaaaagccac acattcgctg gggctgaaaa gtgcggcagc ccggcgggag
     3961 ctgggggtgt taatcagtca gatggcgcgc ggcaattttg gtgcgctgag gggatccggg
     4021 ataacgctgg ctaaccgtgc cgtatggaca ggcgcactga tgtcgccgaa aggcatgatg
     4081 actggcggcg ttattggcgg acttgtcgcg gcggtcctgg gtctgggtaa agcctggcat
     4141 gacggccgga aggagggcga ggaatttaac cgtcagctgg cgctgacggg acattatgcc
     4201 ggtgtcactg tcgggcagtt gtggaaactc agccgggcca tatccgggaa tggtatcacg
     4261 caacatgcgg cagccggtgc gctggcacag gtggtgggca gtggggcatt tcatggaaac
     4321 gatattggta tggtggcgaa agctgccgca cagatggagc gatcggttgg ccagtcggtc
     4381 agcgatacca taaatcagtt taagcggctg aaggatgatc ctgtaaatgc cgcgaaggcg
     4441 ctggacaatg cgctgcattt tttgactgcc actcagcttg agcagatacg cgtccttggg
     4501 gagcaggggc ggtccagtga tgcggcccgg atcgccatgt ctgcgctggc agaggaaacc
     4561 ggtaaacgca catctgatat tgataataat ctcaatgcgc tgggtagtac gctgcaaacc
     4621 ttgtctgact ggtggaagca gttctgggat gcggccatga atattggtcg tgaagactcg
     4681 ctggatgcgc agattgatgc gttacaggaa aaaattcagc gcgcgaaaaa atatccgtgg
     4741 acaaacgcct ccacacaggt ggagtacgat cagcagcgtc ttaacgatct tcaggagaaa
     4801 aaacgccgga aggatttgca ggatgcaaaa gcgcaggcag aacggaatta ccaggagcaa
     4861 cagaaacgcc ggaatgctga aaatgccgtg ctgaaccgga tgaatgaaac ggaagctgca
     4921 cgacatcagc gggaaattgc gcgtattaat gccatgcagt acgccgacca ggctgtcagg
     4981 gatgcggcga tacagcgtga aaatgaacgt tacgaaaaag ccattaagaa aaatacacgg
     5041 gcaacccgta atgatgaggc cacccggtta ttgctgcagt acagtcagca gcaggcacag
     5101 gtggaagggc agattgctgc tgccagacag tcagcaggca ttgccactga aaggatgact
     5161 gaagcgcata aacagcttct ggccctgcag cagcgcatca gcgacctgga cgggaaaaag
     5221 ctgacggcag atgaaaagag tgtgctggcc cgtaagaatg agctgattca ggcgctgacg
     5281 ctgctggatg tgaaacagca ggagctgcag aaacagacag cgcttaacga cctgagaaaa
     5341 aaaacggttc agctgaccag ccagctggca gacaaagaac gtgcactgcg tgagcagcac
     5401 aatctggata ttgccactgc aggtatgggg gataagcagc ggcagcgcta ccaggcacag
     5461 ttgcgcatcc ggcaggaata ccggcaacag ttgcaacagc ttgagaatga cagtcgccag
     5521 aaaggcactt acgggacgga ggactaccgg agggctgagg aggtgctgaa ggggagcctg
     5581 aagcgacaac tgaatgaaaa caaacgctac tggcaggaac tggaagtggc gcagggcgac
     5641 tggaaaaacg gtgccatgcg ggcgtttcag aattttacgg cggatgcgga taatgcggcg
     5701 ggaacggcag aacagatgtt tacagtggca ttcagcagtg ccggtaatgc actggcgaca
     5761 ttctgtacca ccggtaagct gaattttaaa tccttcacct cttccctgtt gtcagatatg
     5821 gccagaatta tggcacagat ggccatgatg caggcggtaa agggcgtcgg ttctttattc
     5881 ggcttcacga ctaatgctga tggcggtgtt taccagtctg ctgatttgag tcgctacagt
     5941 ggcacggtgg ttaaccgtcc gacgtttttt gcttttgcaa aaggagcggg tgtgatgggg
     6001 gaagcgggac ctgaagccat tctgccactg cgtcgcggtg ctgatggtaa gctgggggtt
     6061 gtggcggata ctggtggttc aggcatggtg atgtttgccc cgcagtacaa catcgggatc
     6121 aataacgacg gcacgaacgg gcagataggt ccggcagcac tgaaggtggt ttatgacctc
     6181 gggaaaaaag cggccgcgga ctttatgcaa cagcaggccc gtgatggtgg ccggttaagt
     6241 ggagcatacc ggtaatggag acgtttcgct ggaaggtgcg cccggatatg aatgtggtat
     6301 cagagccaaa agtggtggca gtgaagctgg gtgatggtta tgaacagcgt cgcccggcgg
     6361 gactgaataa tctgctgtcg acttacagcg tgacgatacg tgttcgtaaa ggtgaacacc
     6421 cacctttaaa agcctttctg gaacggcacg gtggcgtccg cgcatttcag tggacgccac
     6481 cttatgactg gaaaccgatc agggtggttt gtcgtaaatg gtcggcaagc gtgggggcgt
     6541 tatgggtgac tgtaacggcc gattttgaac aggtggtgaa ctgatgcagg atatccggca
     6601 ggaaacactg aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat
     6661 cgacctgaca gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg
     6721 tgagccggtc acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt
     6781 tgaactgaat ggcaaaggca ccagtacgcg ccccacgctg acggtttcta acctgtacgg
     6841 tatggtcgcc gggatggcgg aagatctgca gagtctggtc ggcggaacgg tggtccggcg
     6901 taaggtttac gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga
     6961 tccggagcag gaggtgatca gccgctggcg catcgagcag tgcagcgaac tgagcgcggt
     7021 cagtgcctct tttgtgctgg ccacaccaac ggagacggat ggcgtggttt tcccggggca
     7081 tatcatgctg gcgaatacct gtatgtggac ctaccgttct gatgagtgtg gttacacggg
     7141 cagggcagtg gctgacgagt tcgacaaacc aacgacggat atccggaagg acaaatgcag
     7201 caagtgtatg cgcgggtgtg agttgcgcaa caataccggt aatttcggcg gtttcctttc
     7261 catcaataaa ctttctcagt aaatccatga cacagacaga atcagcgatt ctggcgcacg
     7321 cccggcgatg tgcgccagcg gagtcgtgtg gcttcgtggt gagaacgccg gagggagaca
     7381 ggtatcttcc cagcgagaat atctccggtg agccggagga acggttccgg atggctccgg
     7441 aggactggct gcgggcacaa atgcagggtg agattgtggc actggtccac agtcatcccg
     7501 gtggtctgcc ctggctgagt gaggctgacc ggcggctgca ggtgcagagt gatttgccgt
     7561 ggtggctggt ctg
//
LOCUS       DAJTNA010000183         7553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTNA010000183 DAJTNA010000000
VERSION     DAJTNA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.185"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..61)
                     /locus_tag="OO161_003426"
     CDS             complement(<1..61)
                     /locus_tag="OO161_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5464371.1"
                     /translation="MTTTFTGTVSSANSGNYYTI"
     gene            complement(68..328)
                     /locus_tag="OO161_003427"
     CDS             complement(68..328)
                     /locus_tag="OO161_003427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5464372.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            complement(1003..1488)
                     /gene="sixA"
                     /locus_tag="OO161_003428"
     CDS             complement(1003..1488)
                     /gene="sixA"
                     /locus_tag="OO161_003428"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5464373.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1691..3835)
                     /gene="fadJ"
                     /locus_tag="OO161_003429"
     CDS             complement(1691..3835)
                     /gene="fadJ"
                     /locus_tag="OO161_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5464374.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCASHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3835..5145)
                     /gene="fadI"
                     /locus_tag="OO161_003430"
     CDS             complement(3835..5145)
                     /gene="fadI"
                     /locus_tag="OO161_003430"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5464375.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMSVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(5324..5608)
                     /locus_tag="OO161_003431"
     CDS             complement(5324..5608)
                     /locus_tag="OO161_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5464376.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5980..7320
                     /gene="fadL"
                     /locus_tag="OO161_003432"
     CDS             5980..7320
                     /gene="fadL"
                     /locus_tag="OO161_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5464377.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPARQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1965 a         1962 c         1743 g         1883 t
ORIGIN      
        1 aaatggtgta ataattgccg ctatttgcag aagaaacggt accagtaaag gtagtggtca
       61 ttgtttccta ggtatcaaat acgcaatctt gcagacgaat ctggaaactt ttcggcgtgg
      121 tggtgccgtt atttttcagt ttttcggcac cgatatcacc catatcaact tccagtttgt
      181 gatcgtccgg aacgatagaa cacactgagt cttcaatggt gccgtaaaaa cgtgcagtac
      241 cgttgttacc cgcagcgatt gttgccgtag aggtgaacac gcccatcacc attgccgcag
      301 caatagctgt tttaacaaac ttactcatta tttgtcctta aactataaat aattaaaaat
      361 ataaacattg caatacattg attcagtcaa tagccaatgt ttatacccag ccattgaata
      421 cggcatgcga tccaaaaaga acaggtcgta gaaaattgta ttcgttggtg tggggccgca
      481 tagtgatgtt tccacacccc tgatgcaatt taattttact aaagagtaaa tgtagattta
      541 attaatatat tgacagaaga aagattatct taaggacatc cttaaaaaat acggaataag
      601 tatttagaaa aaatagaaag tatatcaata atgaatatca acagcttaaa aaacaaacaa
      661 tgaacatata atgcgattgg catttatcta cttttgacaa tataatatat aaacactata
      721 gccagaattt aatcaagttt caattacatc ctgcctgatt attcaactct attatctgta
      781 ttaataaatg acaatgagtg tcaaacagac attattcatt tcaacatgta actttgggca
      841 aaatcaagat acgcgcagaa gaataatttc cgaatgaatg tagaaagtgt tttatttata
      901 gataaggaga gtcttcgata gtagtgaggt tgacagatat ccgctgcctg atgagcaatt
      961 atgcccaatg aatctacctc attgggcata atttggaaca catcagatag cttttgccat
     1021 cttcagatta cacgggctca tctgccagtt gaatgtacca tttccgcttt catcaagcgt
     1081 tacgctggca atggccgagg tggtaaacat aggcggcgtt tcgcccggac ataactcggc
     1141 aaccagatac ccgactaacg gtaagtgaga gatcaccagt accgaagcaa caccttcatt
     1201 ggtcaacgcc tgcaaatagg cactgaccag accaacatcg ccgcagggcg ttaactccgg
     1261 cagaacttct gcactggaag gcaggttcag acaatcccct acttcttcca gtgtttgctc
     1321 ggctcgcagg aacgggctca ccagaacgcg ttcgatttcc actttttgac ctttcagcca
     1381 gttcgccatc aggcgagatt cgtcacaacc attagtggtc agaggacgaa ccgaatcact
     1441 ggcggcatcg agggctgcgt cgccgtgacg catgataaaa acttgcatat tgcaccgctt
     1501 ttgttaacca gattcgccca ttttctttca tctgaaacca gagaaagaaa acggtggccg
     1561 gcattgtgcc ttatccattc accgaatgaa acgctgtttt ttacctcaat ggcgtaagta
     1621 tagtcaatat gcgtttacat tttgaccaat actccgccat tcagcgcgga ttcatatagc
     1681 tttgaccttc ttattgcagg tcagttgcag ttgttttcca aaaactttcc ccacgcgcgc
     1741 ccatctcgac caaacgctcg caaggggtaa aacgggaacc atactgcgtg gccagtcgtt
     1801 gcattattgc aaccacttcg cccgcgccga gagaatcgat atagcggaac ggtccaccga
     1861 gaaatggcgg aaaaccaatg ccaaataccg caccaatatc cccgtcacgc acgctacgga
     1921 taacctgctc atcaacacaa cgtactgctt cattcagcat caacatcaca caccgttcag
     1981 caacctgcgg tgcggagagt cgcccctgcc cttgtgcgcc aatgagcggg taaatggcgg
     2041 gatcgacctg ttttttgctt ttacgccctt tctgaccata aagatagaaa ccccgaccat
     2101 tttttctgcc tttgcgatcg tcgttcaaaa ttgaagaaac aacatttgca ggcgcgctaa
     2161 aacgttctcc ataagcggct tccagtacag gaataatttt agtcccggtg tcgattccta
     2221 cctcatccaa aagttggatt ggacctaccg gaaaaccaaa tttcactagc gcggcatcaa
     2281 tgtgctctac ccgttcacct ttggtcagca tgcggatagc ttcattaatg taaggcgcta
     2341 agatgcgatt gacgtaaaac ccggctttgt cacgcacgac aatgggcgtt ttgccctgtt
     2401 ttttcgccag ttttaccgta gtggcgatgg tttgcgctga tgtacccgca tgaggaataa
     2461 tctccaccag cggcattttt tccaccggac tgaagaaatg caggccgata acttgctcag
     2521 gtcgcgtggc gtgagcggcg atatcaccaa tcggtaaaga tgacgtattc gaagcaaaga
     2581 tggtatgaga ggcgcaattt tgctcaactt ccgccaccat ctgttgtttc aattcgagat
     2641 tttcaaacac cgcttcaata atcagatcgc gatgggcaaa gccgcgatag tccgtcgttc
     2701 cggagattaa tgccagctgt ttgtcacgtt cgctggcttt gagatgacga cggcgaactt
     2761 tgccctccag ctgatcccaa ctgtacttca gcgcatgatt tatgccctgc gggttgatat
     2821 ctttaattct gaccggaagc cccgctttac aagcagtgac ataagcaata ccgccgccca
     2881 tcaagccacc acctaaaatt cccacgctgt ttaatggcgc aggcggtgca tcactaccgg
     2941 gatctttctt cacgtccgta ctggcaaaaa agatactacg cagcgcctgc gattgtggcg
     3001 tcatcgccag ttcgccaaac gcccgagctt cggcgtcata accgctgctg gtgccctgcg
     3061 ctaatcccgt ttcaacaacc tccaggatgc gttctgtcgc cggataattg ccttgagttt
     3121 tgtgttctgt tttcttgccg accattttga acagcagcgc acgacctaac ggccccgcca
     3181 gaatacgctc gcgtacaggt agagggcggg aagatgggcg atccttcttc gccagctcaa
     3241 cagcgacttc cagcaaaatg gagtgcggaa caacgtcatc caccagcccc agctttaatg
     3301 cctgtttcgc ccgaagttgt tttccggtga ggatcatctc taatgctgtg ctgacgccta
     3361 tcagacgcgg taaacgctgg gtgccgcctg aaccgggtaa caatccaagt tgtacttcag
     3421 gcaaaccgag caccgtttta ggatcgtcag tacaaacgcg accgtggcac gccagcgcca
     3481 actccagccc accacccagg caagcgccat gaatagccgc gataaccgga atgggcaaag
     3541 catgaatctc cgccatcaac tgttggccct gccgcgccag agcttccgct tcttgcgccg
     3601 ttttgcagtt gccgatcatg ttgatgtctg ctccggcgat aaaattgtcc ggtttcgcgg
     3661 agataaacac cacgccgcgc aactctttgt tttcacggag ttgcttaata atggcgcgca
     3721 cctgcgaggc aaattccgcc ttcagggtat tcattttctc atccggtacg tcgatggtga
     3781 tgatagcaat gttgtccaga cgaacattaa gagtaaacgc tgatgccatt tccattattc
     3841 cgcctccaga accattgccg cgccaagccc accggcagca caggcggtaa ctaaaccaaa
     3901 tccaccgccg cgacggcgaa gttcatgcaa tgtctgggta atcatccgcg cgccggtcgc
     3961 cgcgaaggga tgcccgtaag caatcgaacc gccaagcacg ttaaatttgc tatcgtccac
     4021 ttcgccagtg gcatgtgcac gccccagtac ttcacgagca aaacgttcac tacccagcaa
     4081 ctgaatattc gccagtgtct gagctgcaaa ggcttcgtgc atatcgatca atgtcagatc
     4141 gctcattgtc aaaccggcac gttccagcgc cagcggtgtt gaccaggccg gaccgaacaa
     4201 catgtcctgc cagacatcaa tcgcagtaaa tgcgtagctg cgcagatacc ccagcggcac
     4261 cagccctaat tctttcgccc gggattcagt catcaggatc accgctgccg cgccatcggt
     4321 cagcggcgta ctgtttgccg ccgttaccgt tccgtgtttg cgatcaaacg ccgggcgcag
     4381 ctttgcgtaa tcggcaagcg aggaattacc gcgaatattg ttgtcttcga caagcggttg
     4441 tttataagga gggataaagg cagtcatcac ctcttctttg agttttccgt ctgaccatgc
     4501 ctgagcggca cgctgatgcg aacggtgcgc taatgcatct tgctgttctc gggtgatgcc
     4561 gtaggttttc gccatttgct ctgcggtgtc acccatccgc aagccggtag aatattctgc
     4621 tactgcaggc ggtacggaca ttaaatcgcg caaacgcagg cgagagaaga gtttcaggcg
     4681 ctggctcatg gttcgagctt tgttgacatc aaccagcacg cgcgccagtt ttttactgac
     4741 gccaattggc aatacggaag aggaatctgc cccaccggca atccccgctc ggatagttcc
     4801 cgccatcagg ctttctgcga cgtttgcaac cgcctggaaa ctggtagcgc aagcgcggct
     4861 gacgctgtaa gcatcggtat gcacattcat tccggtaccg agaacaattt cacgcgcaat
     4921 gttgggggct tcaggcattt gtacgacctg accaaagacc agctgttcaa tcacttcagc
     4981 ggggatctcg ctgcgtgcca gcagttcgcc taccaccatc ttccctaaat caaccgcggg
     5041 aatgccatga aaagccgtcg cctggcgggc aaaaggcgta cgtaaaccgc taacaatggc
     5101 gatacgatcg ccctggcggg taaccagcgg taaaacctga cccataacac tcccctgtaa
     5161 aaaaataaat aaagtggtct gacctgatca tagtcttaac cattttttac atttagccaa
     5221 gcggagaaaa aggaaagtgg gagctatgac acagtgaaga caggagaaag aaaagaaaac
     5281 gcccctgcca gcaactgaca ggggcgaacg tacccgagag gaattaacgc agacccagct
     5341 ggaagatcag catttcagct tcgcaggcaa aagtgaaatc gatatccagg cgcacaccgt
     5401 cagactcttc agtgaaagtc ggggagattt tgcacggttc ggattccacg ctacgggctt
     5461 tttcagtcaa cgccgccagc gtttgttctg cttctgcgcg gtttgcaaac acgcggctgt
     5521 aagacgcggt gcagtcggag ttgtccataa tggtgccaac atccatacag cagcaaaccg
     5581 gggtttcatc agcactacat ttactcatcg ttgatttcct ctgtatgtgc acccaaggtg
     5641 ccagataaac gttgtggata ttttacgctt ccggaaagtg ctgctccagt tgttaattct
     5701 gcaaaatcgg ataagtgacc gaaatcacac ttaaaaatga tctaaaacaa aattcacccg
     5761 aatccgcgag tgcgcaacct ccaaattttg ccagctggat cgcgtttctt agatcatatt
     5821 tgaaaaaaga tggaaacata cttgcaacat tccagctggt ccgacctata ctctcgccac
     5881 tggtctgatt tctaagatgt acctcagacc ctacacttcg cgctcctgtt acagcacgta
     5941 acatagtttg tataaaaata aatcattgag gttatggtca tgagccagaa aaccctgttt
     6001 acaaagtctg ctctcgcagt cgcagtggca cttatctcca cccaggcctg gtcggcaggc
     6061 tttcagttaa acgaattttc ttcctctggc ctgggccggg cttattcagg ggaaggcgca
     6121 attgccgatg atgcaggtaa cgttagccgt aaccccgcat tgattaccat gtttgaccgc
     6181 ccgacatttt ctgcgggtgc ggtttatatt gacccggatg tgaatatcag cggaacgtct
     6241 ccgtctggtc gtagcctgaa agccgataac atcgcgccta cggcatgggt tccgaacatg
     6301 cactttgttg caccgattaa cgaccaattt ggttggggcg cttctattac ctctaactat
     6361 ggtctggcaa cagagtttaa tgatacttat gcaggcggct ctgtcggggg tacaactgac
     6421 cttgaaacca tgaacctgaa cttaagcggt gcgtatcgct taaataatgc atggagcttt
     6481 ggccttggtt tcaacgccgt ctacgctcgc gcgaaaattg aacgtttcgc aggcgatctg
     6541 gggcagttgg ttgctggtca gattatgcaa tctcctgcca ggcaaactcc tcaagggcaa
     6601 gcattggcag ctaccgccaa cggtatcgac agtaatacca aaatcgctca tctgaacggt
     6661 aaccagtggg gctttggctg gaacgccgga atcctgtatg aactggataa aaataaccgc
     6721 tatgcactga cctaccgttc tgaagtgaaa attgacttca aaggtaacta cagcagcgat
     6781 cttaatcgtg cgtttaataa ctacggtttg ccaattccta ccgcgacagg tggcgcaacg
     6841 caatcgggtt atctgacgct gaacctgcct gaaatgtggg aagtgtcagg ttataaccgt
     6901 gttgatccac agtgggcgat tcactatagc ctggcttaca ccagctggag tcagttccag
     6961 cagctgaaag cgacctcaac cagtggcaac acgctgttcc agaaacatga aggctttaaa
     7021 gatgcttacc gcatcgcgtt gggtaccact tattactacg atgataactg gaccttccgt
     7081 accggtatcg cctttgatga cagcccagtt ccggctcaga atcgttctat ctccattccg
     7141 gaccaggacc gtttctggct gagtgcaggt acgacttacg catttaataa agatgcttca
     7201 gtcgacgttg gtgtttctta tatgcacggt cagagcgtga aaattaacga aggcccatac
     7261 cagttcgagt ctgaaggtaa agcctggctg ttcggtacta actttaacta cgcgttctga
     7321 taacgcgttc gcctggataa agtcacctgc atagcaggtg actttaactc ccccacttca
     7381 ccgaaagtag tgtcctcatt gcttacctcc tgagttttgc aaacagcctg ttggcagctt
     7441 gccctttcaa atcaataagc ggtatccaca gacaaacctg aagggaaagg catattttca
     7501 ggcgttctgc tcgtccttct caaagagttt acttttctgc atttccagga tac
//
LOCUS       DAJTNA010000184         7445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTNA010000184 DAJTNA010000000
VERSION     DAJTNA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.186"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..265
                     /locus_tag="OO161_003433"
     CDS             <1..265
                     /locus_tag="OO161_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HCR5464378.1"
                     /translation="RDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSK
                     MRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            628..1665
                     /gene="dusA"
                     /locus_tag="OO161_003434"
     CDS             628..1665
                     /gene="dusA"
                     /locus_tag="OO161_003434"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5464379.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1799..2041
                     /gene="pspG"
                     /locus_tag="OO161_003435"
     CDS             1799..2041
                     /gene="pspG"
                     /locus_tag="OO161_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5464380.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2206..3189)
                     /locus_tag="OO161_003436"
     CDS             complement(2206..3189)
                     /locus_tag="OO161_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5464381.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3272..4687
                     /gene="dnaB"
                     /locus_tag="OO161_003437"
     CDS             3272..4687
                     /gene="dnaB"
                     /locus_tag="OO161_003437"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5464382.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4740..5819
                     /gene="alr"
                     /locus_tag="OO161_003438"
     CDS             4740..5819
                     /gene="alr"
                     /locus_tag="OO161_003438"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5464383.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6072..7265
                     /gene="tyrB"
                     /locus_tag="OO161_003439"
     CDS             6072..7265
                     /gene="tyrB"
                     /locus_tag="OO161_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5464384.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1776 a         1803 c         1973 g         1893 t
ORIGIN      
        1 acgtgatatg aagaagtacc tgggagagat gaagcgtctg gctgatgggg gagaaatgaa
       61 ttttgatggc tatctggata agatgtcaca tctggtgaat gaagggacgc tcgatcctga
      121 tattttaagc aaaatgcgag atgctgcacc acaattaatt agcttcgcga agtcgtttga
      181 cccaacctca aaggaagaga ttaaaatact tacagacact tctaaattaa tttatgattt
      241 gttcggggtt aaatcggaga aataatatgt gaagttcttc gatagtatgg aaggcattat
      301 ataaaaggac ccaatattta ttgggttctt ttttctctat caatgctatt agcagggaga
      361 tatatcacca gagtttaatg tgtgattttt tatttatcgt cgaacctgga ttgtttatca
      421 ttggccttaa caaagttaac ggctaataag attatttcca tcacttcgtg agagcttcat
      481 gccttgagag gatctcaatt ttcttttgca atgagacagg cgcttcctgt tgttatggta
      541 tagtaccccg ctattgagcc tcctgaatag tgatgctgaa taacataacc ccatgatata
      601 tcgataaaat aatctctaca tttgaaaatg cacggtaatt ctgaaatgca aaaaatcaac
      661 caaaccagcg caatgcctga aaaaactgac gttcactgga gtggtcggtt tagcgttgca
      721 ccaatgctcg actggacgga cagacattgc cgctatttct tgcgtctgct ttcccgcaat
      781 acgttgctgt ataccgaaat ggtgaccaca ggggcgatta ttcacggtaa aggtgattac
      841 ctggcgtaca gtgaagaaga acatccggta gcgttgcaac tcggcggtag cgatccggcg
      901 gcgctggcac agtgtgcgaa gctggcagaa gcgcgtggat atgatgagat caacctgaat
      961 gtcggctgcc cgtctgaccg ggtgcagaac ggcatgtttg gtgcgtgtct gatgggtaat
     1021 gcgcagctgg ttgccgattg cgtgaaagcg atgcgcgatg tggtgtcgat tccggtgacg
     1081 gtgaaaacgc gtattggcat cgatgaccag gacagctatg aatttctctg cgatttcatc
     1141 aacaccgttt ccggcaaagg cgagtgtggg atgttcatca tccatgcacg taaagcctgg
     1201 ctttcggggt taagcccgaa agaaaaccgt gaaatcccgc cgctcgatta tccgcgtgtg
     1261 tatcaactga agcgtgactt tccgcatctg acaatgtcga ttaacggtgg tatcaagtcg
     1321 ctggaagagg ccaaagcaca cctgcaacat atggatggcg tgatggtcgg gcgcgaggcg
     1381 tatcagaatc cgggtattct ggcggcggta gaccgggaga tctttggttc ctcggatacc
     1441 gatgcctatc cggtggcggt agtgcgcgcc atgtatccgt acattgagcg tgaactcagc
     1501 caggggacgt atctcggcca tattacccgg catatgttgg gcttgttcca gggtattcct
     1561 ggcgcgcggc agtggcggcg ttatttaagt gaaaatgccc ataaagcggg tgcagacatt
     1621 aatgtgctgg aacacgcgct caaactggtg gcggataagc gttaactttt caccaaaaag
     1681 tagtcaaatt caccacgccc tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga
     1741 ttatttttgg catgattctt gtaatgccag caagagattt catatttggg agagcatcat
     1801 gctggaacta ctttttgtga ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt
     1861 gctgggcatt atcgccgcgc tggttgtggc gacggccatt atgttcctcg gcggtatgct
     1921 ggcattgatg attaagttgc tgccgtggtt actactggcg attgcggtgg tgtgggttat
     1981 taaggcgatt aaagcaccaa aagtgcctaa atatcagcgt tatgaccgct ggcgttacta
     2041 agggattgtg cggatgatca caacctaagg ttttatcctt agaacaaaat aggaattgat
     2101 aatcaaatct gtcactattg cgcctctaac agattcatcg tgctgtaccc tacatacagc
     2161 cgactataaa aagaaagggc ttcccaggtg gaagccctat ttcttttatg gaatcagcag
     2221 gctggaacct tgcgtcgccc ggctttccag aatctcatgc gcacgctgcg catccttcag
     2281 cggatatttc tgctgctcgg cgacatcgac cttaatcaca ccgctggcaa tcaaagagaa
     2341 cagttcatta ctggcctcgg ttaattcctc ccgcgtggtg atatagcctt gcagggaagg
     2401 gcgtgtcaca tacaacgagc ctttttgatt gagaatgcct aagttcacac cggtaaccgc
     2461 acctgatgag ttgccaaaac tgaccattaa gccgcggcgt tgcaggcaat ccagcgaccg
     2521 ttcccaggtg tctctgccca cggaatcgta cactacgcgc actttcttac cgccggtgat
     2581 ctctttcaat cgctcgacca gattctcttc acgatagtta ataacctgcc acgcgccggc
     2641 ttttagcgcg ctctgcgctt tttgcgcggt tcctacggtg ccgataagtt tcgcgcccag
     2701 agcttttgcc cactggcagg caatcaagcc aacgccgcca gccgctgcgt ggaacaggaa
     2761 ctgttcatcg ggtttaattt cataggtttt gcgcagcaga taataaaccg ttaagccttt
     2821 caggaaagat gctgcagctt gctcaaaaga aatagccgca ggcagaatcg ccgctttatc
     2881 cgcattaatg ttatgcacag agctgtaagc gcctaacgcc gactgcgcat agactacacg
     2941 atcgcctgcc ttaatatgct ttacaccact gccgacttta ctcacgatgc ctgccgcttc
     3001 ggtacctgat ccgctgggta gtgatggcgg cgggtaaagg ccgctgcgga tatacgtgtc
     3061 gataaaattg atgccgatgg ctttattttc gacctggatt tcattctctg ccggatcggc
     3121 aggagtgaac tctacggctt gaagtacttc cgggccaccg tgcttgtgaa attcaattcg
     3181 tgttgccatg tgtcctccag aacgtatcgt cagggtctgc ttcatatggt aaagtttcga
     3241 cccattcttt atctcggtaa ctccattcac tatggcagga aataaaccct tcaacaaaca
     3301 gcaggctgaa ccccgcgaac gcgatccaca agttgccggg ctgaaagtgc ctccgcactc
     3361 gatcgaagcg gagcagtcgg tgttgggcgg tttaatgctg gataacgaac gctgggacga
     3421 tgtagccgag cgtgtggtgg cagacgattt ttacacccgc ccacaccgcc atatctttac
     3481 tgaaatggcg cgtttgcagg aaagcggtag tcctatcgat ctgattaccc ttgcggaatc
     3541 gctggaacgc caggggcaac tcgatagcgt cggtggtttt gcttatctgg cagagctgtc
     3601 aaaaaatacg ccaagtgcgg cgaacatcag tgcttatgct gacatcgtgc gtgaacgtgc
     3661 cgttgtccgt gagatgatct cggttgcgaa tgagattgct gaagccggtt ttgatccgca
     3721 ggggcgtacc agcgaagatc tgctggacct tgctgaatcc cgcgtcttta aaattgccga
     3781 aagtcgtgca aacaaagacg aagggccgaa gaacatcgcc gatgtgctcg acgcaaccgt
     3841 ggcgcgtatt gagcagttgt ttcagcagcc acacgatggc gttaccggag taaacaccgg
     3901 ttatgacgat ctcaacaaaa aaaccgctgg cttgcagccg tcggatttga tcatcgtcgc
     3961 cgcgcgtccg tcgatgggta aaacaacatt tgcgatgaac ctcgtcgaaa acgcggcgat
     4021 gttgcaggat aaaccagtac ttatcttctc gctggagatg ccttcagaac agattatgat
     4081 gcgttctctg gcgtcgctgt cgcgcgttga ccagactaaa atccgtaccg ggcagctcga
     4141 tgatgaagac tgggcgcgca tttccggcac catgggtatt ttgctcgaaa aacgcaatat
     4201 ctatatcgat gactcctccg gcctgacgcc aacggaagtg cgttcccgcg cacgccgtat
     4261 tgcccgtgaa cacggcggca tcgggcttat catgatcgac tacctgcaac tgatgcgcgt
     4321 accggcgctt tccgataacc gtacgctgga aattgcagaa atctctcgct cgctgaaagc
     4381 actggcgaaa gaactgaacg tgccggtggt ggcgctgtcc cagttgaacc gttctctgga
     4441 acaacgtgcc gacaaacgcc cggtcaactc cgacctgcgt gaatctggct ctatcgagca
     4501 ggatgcggac ttgatcatgt ttatctatcg tgatgaggtg tatcacgaaa acagtgattt
     4561 aaaaggcatc gcggaaatta ttatcggtaa acaacgtaac ggcccaatcg ggacggtacg
     4621 cctgaccttt aacggtcaat ggtcgcgctt cgacaactat gcggggccgc agtacgacga
     4681 cgaataataa ttattttatg aattaggtaa ttaaagcaaa cacttatcaa ggaacacaaa
     4741 tgcaagcggc aactgttgtg attaaccgcc gcgctctgcg acacaacctg caacgtcttc
     4801 gtgaactggc ccctgccagt aaaatggttg cggtggtgaa agcgaacgct tatggtcacg
     4861 gtcttcttga gaccgcgcga acgctccccg atgctgacgc ctttggcgta gcccgtctcg
     4921 aagaagctct gcgactgcgt gcggggggaa tcaccaaacc tgtactgtta ctcgaaggct
     4981 tttttgatgc cagagatctg ccgacgattt ctgcgcaaca ttttcatacc gccgtgcata
     5041 acgaagaaca gctggctgcg ctggaagagg ctagcctgga cgagccggtt accgtctgga
     5101 tgaaactcga taccggtatg caccgtctgg gcgtaaggcc ggaacaggct ggggcgtttt
     5161 atcatcgcct gacacagtgc aaaaacgttc gtcagccggt gaatatcgtc agccattttg
     5221 cgcgcgcgga tgaaccaaaa tgtggcgcaa ccgagaaaca actcgctatc tttaatacct
     5281 tttgcgaagg caaacctggt caacgttcca ttgccgcgtc gggtggcatt ctgctgtggc
     5341 cacaatcgca ttttgactgg gtgcgcccgg gcatcattct ttatggcgtc tcgccgctgg
     5401 aagatcgctc caccggtgcc gattttggct gtcagccagt gatgtcacta acctccagcc
     5461 tgattgccgt gcgtgagcat aaagccggag agcctgttgg ttatggtgga acctgggtaa
     5521 gcgaacgtga tacccgtctt ggcgtagtcg cgatgggcta tggcgatggt tatccgcgcg
     5581 ccgctccatc cggtacgcca gtgctggtga acggtcgcga agtaccgatt gtcgggcgcg
     5641 tggcgatgga tatgatctgc gtagacttag gtccacaggc gcaggacaaa gccggggatc
     5701 cggtcatttt atggggcgaa ggtttgcccg tagaacgtat cgctgaaatg acaaaagtga
     5761 gcgcttacga acttattacg cgcctgactt caagggtcgc gatgaaatac gtggattaat
     5821 cgttctgtaa tatttgattg tctgtgccgg atgcggcgtg aatgccttat ccggccaata
     5881 aaatcctaaa aattcaataa gttgatgttc tttcatgctc ttataaaggt cgtgcctctg
     5941 gcggatgttc gtttgtcatg agtctcactc tgttgctaat tgccgttcgc tcctgaacat
     6001 ccactcgatc ttcgccttct tccggtttat tgtgttttaa ccacctgccc gtaaacctgg
     6061 agaaccatcg cgtgtttcaa aaagttgacg cctacgctgg cgacccgatt cttacgctta
     6121 tggagcgttt taaagaagac cctcgcagcg acaaagtgaa tttaagtatc ggtctgtact
     6181 acaacgaaga cggaattatt ccacaactga aagccgtggc ggaggcggaa gcgcgcctga
     6241 atgcgcagcc ccatggcgct tcgctttatt taccgatgga agggcttaac agctatcgcc
     6301 atgccattgc gccgctgctg tttggtgcgg accatccggt actgcaacaa cagcgcgtag
     6361 caaccattca aacccttggc ggctcagggg cattgaaagt gggcgcggat ttcctgaaac
     6421 gctacttccc ggaatcaggc gtctgggtca gcgatcctac ctgggaaaac cacgtagcaa
     6481 tattcgccgg ggctggattc gaagtgagta cttacccctg gtatgacgaa gcgactaaca
     6541 gcgtgcgctt taatgacctg ttggcgacgc tgaaaacatt acctgcccgc agtattgtgt
     6601 tgctgcatcc atgttgccac aacccaacag gtgccgatct cactaatgat cagtgggatg
     6661 cggtgattga aattctcaaa gcccgcgagc ttatcccatt cctcgatatt gcctatcaag
     6721 gatttagtgc cggtatggaa gaggatgcct acgctattcg cgccattgcc agcgctggat
     6781 tacccgctct ggtgagcaat tcgttctcga aaattttctc cctttacggc gagcgcgtcg
     6841 gcggactttc tgttctgtgt gaagatgccg aagccgctgg ccgcgtactg gggcaattga
     6901 aagcaacagt tcgccgcaac tactccagcc cgccgaattt gggtgcgcag gtggtggctg
     6961 cagtgctgaa tgacgaggca ttgaaagcca gctggctggc ggaagtagaa gagatgcgta
     7021 ctcgcattct ggcaatgcgt caggaattgg tgaaggtatt aagcacagag atgccagaac
     7081 gcaatttcga ttatctgctt aatcagcgcg gcatgttcag ttataccggt ttaagtgccg
     7141 ctcaggttga ccgactacgt gaagaatttg gtgtctatct catcgccagc ggtcgcatgt
     7201 gtgtcgccgg gttaaatacg gcaaatgtac aacgtgtggc aaaggcgttt gctgcggtga
     7261 tgtaatgcag gaaagcaggc tggagttacc cagcctgcag tgaaattaaa ctgtcgtcgc
     7321 tttcactatt tctttgtaga tgattttttt gatgccgttg ttctacgtga gagataataa
     7381 aacgttgtca gttcttttat tgttaagttt atcccaatta actggaattc ctttccggta
     7441 atgct
//
LOCUS       DAJTNA010000185         7390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTNA010000185 DAJTNA010000000
VERSION     DAJTNA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.187"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            191..512
                     /locus_tag="OO161_003440"
                     /pseudo
     CDS             191..512
                     /locus_tag="OO161_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(546..752)
                     /locus_tag="OO161_003441"
     CDS             complement(546..752)
                     /locus_tag="OO161_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464385.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1250..1323)
                     /locus_tag="OO161_003442"
     tRNA            complement(1250..1323)
                     /locus_tag="OO161_003442"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1289..1291),aa:Gly,seq:ccc)
     gene            complement(1402..2157)
                     /locus_tag="OO161_003443"
     CDS             complement(1402..2157)
                     /locus_tag="OO161_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5464386.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKTGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            2572..4869
                     /gene="xdhA"
                     /locus_tag="OO161_003444"
     CDS             2572..4869
                     /gene="xdhA"
                     /locus_tag="OO161_003444"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5464387.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIVHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASHQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4880..5758
                     /gene="xdhB"
                     /locus_tag="OO161_003445"
     CDS             4880..5758
                     /gene="xdhB"
                     /locus_tag="OO161_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5464388.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5755..6234
                     /gene="xdhC"
                     /locus_tag="OO161_003446"
     CDS             5755..6234
                     /gene="xdhC"
                     /locus_tag="OO161_003446"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5464389.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(6274..>7390)
                     /locus_tag="OO161_003447"
     CDS             complement(6274..>7390)
                     /locus_tag="OO161_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR5464390.1"
                     /translation="QHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPD
                     EPHIEQLVGECRVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKP
                     FIAINCAAIPEQLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLML
                     QAKLLRAIEAREILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPI
                     TLPPLRERQEDIELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLM
                     EYLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQM
                     IREALSRHNSKKQVADELGIGIATLYRKIKKYELLNT"
BASE COUNT         1883 a         1859 c         1765 g         1883 t
ORIGIN      
        1 agtattaaga ctatcactta tttaagtgat actggttgtc tggagattca gggggccagt
       61 ctaataattt cgaaagcaac tcatttttac tctgcaaaca atcagaaaag acttaatttt
      121 gctaagttaa tgcattaatc taaaaacgct ttaaatcaat acataaccat tatctcttgg
      181 gattatttac atgcgcaaaa gtttaatgct gttacttgct tcaacattga ttatttctgg
      241 atgtgcaaca aaaaaacaac tcgttccaac tggtggaagc aaatcagacg gcacagttag
      301 gatgagttat tcttacggta tgtttgaaag tcctgttgta gatccacaac aaggcatgaa
      361 tgcagccaaa gcgcgttgtg ccgcttaggg gatattcagg agccgagcct tttggtggat
      421 tcacttctca atgttcacaa ccttcttcat ctggttgtat gcaaacactt gtgactatcg
      481 aatatcaatg cactggcgat attaagaagt aataattagg ggcttccagc cccttctata
      541 accattcatg gaacagggac aaccgaaatc actttaaacc ctgaaacagc cgttactcca
      601 ttagatggag aattaaatct cacagtatct tttcttactg catcatgaat ttcatctttt
      661 gttggttcat gctctgaatg gaataaaacc tcttccattc taatttgatc accgtctcta
      721 tgctcataag taatcttgta gctaacagtc atatcaacct catgcagggg aattgtgaga
      781 ttattataga caatagaaac cataaagccc cgttggtgat acagcaacaa gcgggagcgt
      841 agcgacttca tcagattgtc agaaagatca caccttatca ggataaccgt gtacccgttg
      901 ggagcgtggc tgaaacgctg atagagcttg catatggttt gcaaggacca atatcaccat
      961 aaacctgttt acgcagccac aaggccgcac atcgggctta aatgcgtgtg ttcagtctgt
     1021 cgatgacatt acaatgccag gatgggcttt atcttcacgt tgtgctatcg cggtgtgaga
     1081 ggtgcaactg ccgccaacgt taggttatac tgtcatctca ccgtgtaact taacgtgaca
     1141 gttactaagg gcggatcaca atggtagaac tcctaagcaa attaaatagg taactgttta
     1201 agttgctgac agagaagact tttttagtct tttccacctg gggagatttt ggagcgggcg
     1261 aagggaatcg aaccctcgta tagagcttgg gaagctctcg ttctaccatt gaactacgcc
     1321 cgcttcgaga tgtgtaaggc attataaacc ttacgctctc cttagcaagt gccgcgctgc
     1381 tgactgctga ttaattcgcc atcagcattt tggcttgctg ccctgcggcg gcaagtaacg
     1441 tagcggatca attgccgttg cacggtaacg aatctggaaa tgcaggcgaa cagatgccgc
     1501 atccgtgctc cccatagtgg cgattttttg cccagtcttc acgctttgcc cattatttac
     1561 cagcatcgtg tcattatggg cgtaagccgt aatgtaatct tcactgtgtt taatcatgat
     1621 gagattaccg tagccacgca gctggttgcc cacatacacc acctttcctg cacccgcggc
     1681 gtaaataggt gtaccccgtg gagctgagat atcaatccct ttattgccgc catctgctgt
     1741 cgaatacggc atgataactt tccctgtcgt tggccataac caacaacgtt gccctactgg
     1801 cggccaggaa gatttcggta ccgctgatga cggtgtaacc gatgcggttt tggtcgttga
     1861 tttggcggtt gatttacgtg tactactgct acttttcgcc ccacccagtt ttagtttctg
     1921 accaacttca atggtgtaag ggggggaaat gccgttcagt cgcgccagtt cttttacgct
     1981 ggttcccgtg gtgcgcgaaa tacgatatag cgtatccccc cgtttcacgg tgtaaacgga
     2041 gccggaatac gttcctgtat cggatgattt gctacccgaa cagcccgcca aaagcagtcc
     2101 aaccgataac aacatcacga tacccagaga ttttttattc aggcgtcccg cactcaaaat
     2161 ctttcctcgc ttaaaaaaca agacgcctta tcatagcagc cagcctcttg atacccaatc
     2221 tcttgcttct ggaaaaacgt ggtaatagag aaagaggtta atttcatttg gcaaagggaa
     2281 gtatcatcag caaaatattc gctgtagagg tatcgaactt cctgactttt taccctattt
     2341 cccatcctga tgcgtaataa tcccaattta tcacattgat tttatcgcca ctcatattga
     2401 tacgtatcac tgcccctatt cagactcctg tttcgtatca tatctgtgtt tttttaacgt
     2461 catcacactt cctacctccc ctaacgctta tcgtcgtttc tggcgtaaat cttgcctgct
     2521 tagactaaat ctttgccata agaaccaaat gttcaaatcg aggggatttc tatggaagcg
     2581 cgggaagcaa ccgctacggg tgaatcatgc atgcgcgtcg atgccattgc taaggtcacc
     2641 gggcgggcac gatatactga cgattatgtt atggcgggca tgtgttatgc gaaatatgta
     2701 cgtagcccta tcgtacatgg ttatgccgta agtattaatg atgaacaagc cagaagtttg
     2761 ccgggcgtac tggcgatttt tacctgggaa gatgtgcctg atattccatt cgctacagct
     2821 gggcatgcct ggacacttga cgaaaacaag cgcgataccg ccgatcgcgc actgctaact
     2881 cgccatgttc gtcatcatgg tgacgccgtt gccatcgtcg tggcccgcga tgaactcacg
     2941 gcagaaaaag cggcgcaatt ggtcagcatt gagtggcaag aattacccgt tatcaccacg
     3001 ccagaagcgg cgctggcaga agacgctgca ccaatccata acggtggcaa tttactgaaa
     3061 caaagcacga tgtcgacggg taatgtccaa caaacaatcg atgccgccga ctaccaggta
     3121 caggggcact atcagacccc cgttattcaa cattgtcaca tggaaagcgt aacatcgctg
     3181 gcgtggatgg aggatgactc gcgaattacc atcgtttcca gcacccagat cccgcacatt
     3241 gttcgccgcg tggttggtca ggcgctggat attccctggt catgcgtacg agtcatcaaa
     3301 ccatttgtcg gtggcggttt tggtaataaa caggatgtac tggaagagcc aatggcggca
     3361 ttcctgacca gcaagcttgg cggcattccg gtgaaagttt cccttagccg tgaagagtgt
     3421 ttcctcgcaa cccgtacccg ccacgctttt accattgacg ggcaaatggg cgtgaaccgc
     3481 gacggaacat tgaaaggtta tagtctggat gttctgtcta acaccggcgc ttatgcatct
     3541 cacgggcact ctatcgcttc tgcggggggg aataaagtcg cttaccttta tcctcgttgt
     3601 gcctacgctt acagttcaaa gacctgctat accaacctcc cctcggctgg tgcgatgcgt
     3661 ggttatggcg cgccacaagt cgtatttgcc gttgagtcta tgcttgatga cgccgcgaca
     3721 gcgttaggta ttgatcctgt tgaaattcgt ttacgcaacg ccgcccgcga aggagatgct
     3781 aatccgctca cgggcaaacg tatttacagc gcagggttgc cggagtgtct tgaaaaaggc
     3841 cggaaaatct ttgaatggga aaaacgccgt gcagaatgcc agaaccagca aggcaatttg
     3901 cgccgcggcg ttggcgtcgc ctgttttagc tacacctcta acacctggcc tgtcggcgta
     3961 gaaatagcag gcgcgcgcct tctgatgaat caggatggaa ccatcaacgt gcaaagcggc
     4021 gcgacggaaa tcggtcaggg tgccgacacc gtcttctcgc aaatggtggc agaaaccgtg
     4081 ggggttccgg tcagcgacgt tcgcgttatt tcaactcaag ataccgacgt tacgccgttc
     4141 gatcccggca catttgcctc acaccagagc tatgttgccg cgcctgcgct gcgcagtgcg
     4201 gcactattat taaaagagaa aatcatcgct cacgccgcag tcatgctaca tcagtcagcg
     4261 atgaatctga ccctgataaa aggccatatc gtgctggttg aacgaccgga agagccgtta
     4321 atgtcgttaa aagatttggc gatggacgct ttctaccacc ctgaacgcgg cgggcagctc
     4381 tctgctgaaa gctccatcaa aaccaccact aacccaccgg cgtttggctg tacctttgtt
     4441 gatctgacgg tcgatattgc gctgtgcaaa gtcaccatca accgcatcct caacgttcat
     4501 gactcagggc atattcttaa tccactgctg gcagaaggtc aggtacacgg cggaatggga
     4561 atgggcattg gctgggcgct atttgaagag atgatcatcg atgctaaaag cggcgtggtc
     4621 cgtaacccca atctgctgga ttacaaaatg ccgaccatgc cggatctgcc acaactggaa
     4681 agcgcattcg tcgaaatcaa tgagccgcaa tccgcatacg gacataagtc actgggtgag
     4741 ccaccaataa ttcctgttgc cgctgctatt cgtaacgcgg tgaagatggc taccggtgtt
     4801 gcaatcaata cactgccgct gacgccaaaa cggttatatg aagagttcca tctggcagga
     4861 ttgatttgag gataacatca tgtttgattt tgcttcttac catcgcgcag caacccttgc
     4921 cgatgccatc aacctgctgg ctgacaaccc gcaggccaaa ctgctcgccg gtggcactga
     4981 cgtactgatt cagctccacc atcacaatga ccgttatcgc catattgttg atattcataa
     5041 tctggcggag ctgcggggaa ttacgctggc ggaagatggc tcgctacgta tcggctctgc
     5101 aacgacattt acccagctaa tagaagatcc tataactcaa cgtcatctcc cggcgttatg
     5161 tgctgcggcc acgtccattg ctggactgca gatccgtaac gtcgctacct acggtggaaa
     5221 tatttgcaac ggtgccacca gcgcagattc tgccacgcca acgctaattt atgacgcgaa
     5281 actggagatc cactccccgc gcggtgttcg tttcgtcccg attaatggct ttcacaccgg
     5341 gccgggcaaa gtgtctcttg agcatgacga aatcctcgtc gcctttcatt ttccgccaca
     5401 gccgaaagaa cacgcgggca gcgcgcattt taaatatgcc atgcgcgacg caatggatat
     5461 ttcaacgatt ggctgcgccg cacattgccg actggataac ggcaatttca gcgaattacg
     5521 cctggcattt ggtgttgccg cgccaacgcc gattcgctgc caacatgccg aacagactgc
     5581 acaaaatgcg ccattaaacc tgcaaacgct ggaagctatc agcgaatctg tcctgcaaga
     5641 tgtcgccccg cgttcttcat ggcgggccag taaagagttt cgtctgcatc tcatccagac
     5701 gatgaccaaa aaagtgatta gcgaagccgt cgccgcggcg gggggaaaat tgcaatgaat
     5761 cacagcgaaa caattaccat cgaatgcacc attaacggga tgccttttca gcttcacgcc
     5821 gcaccaggca cgccgctctc ggaattactc cgcgaacaag gactgctaag tgtcaaacaa
     5881 gggtgctgcg tgggtgaatg tggtgcctgt acggtgttgg tcgacggcac agcaatagac
     5941 agttgcttat accttgccgc ctgggctgaa ggaaaagaga tccgcacgct ggaaggtgaa
     6001 gcgaaaggcg gaaaactttc tcatgttcag caggcttatg cgaaatccgg cgcagtgcag
     6061 tgcgggtttt gtacgcctgg cctgattatg gctaccacgg caatgctggc gaaaccacgc
     6121 gagaagccat taaccattac ggaaattcgt cgcggactgg cgggaaatct ttgtcgctgc
     6181 acggggtatc agatgattgt aaatacagtt ctggattgcg agaaaacgaa gtaaaaggat
     6241 atccggcctg aattcaggcc ggattcactg aggttatgtg tttaacaact catatttctt
     6301 aatcttgcga tagagcgtag caatgccgat gcccagttca tcagcaactt gcttcttgct
     6361 gttatgacgt gaaagcgcct cgcggatcat ttgcttttcc atctcctcca gcgccgtgcc
     6421 gcccgcatca tcgagtgata ggtgcgcctc actgacctct gttacatcac tttgctccgt
     6481 tgtgccatta ttcagcagat ttggcggcaa tagcgtgctg tcgataactt cacctgaagg
     6541 aactacgtta accagatatt ccatcaaatt gcttaactcg cgcaggtttc cgggccaacg
     6601 atgcttacgc aatatttcga cgacatcggg agcaatgcca ggataaaccg atcccagacg
     6661 acgggtatgc agatgtaaaa agtaatgcac caatagttca atatcttcct gacgttcacg
     6721 cagcggtggc agagttatcg ggataacatt aagtcggtag aagagatctt cgcggaattt
     6781 accttcggca atgaactggg ccaaattctg attagttgca gaaatgatgc gaatgtcgac
     6841 ttgtattggg ctactggcac caatcggcag aatttcacgc gcctcaatag cgcgcagtaa
     6901 tttagcctgc aacattaatg gcatatcacc tatttcatcg agaaacagcg tgcccgtatt
     6961 cgccgcctga atcaaccctg ttttaccgtt ggcagaagcg ccagtaaatg cgcctttaac
     7021 ataaccgaac agttcgcttt ccagaagctg ctccggaatc gcggcacagt tgatagcaat
     7081 aaagggttta ttccgtcttc cgctcaactt atggattgct cgggcgacga cttctttacc
     7141 cgtgccgctt tcaccaacca ccataacgct ggatgggctg ggtgcaatac ggctaatgag
     7201 tcgttttaat tgccgcataa cacggcactc gccaaccaat tgttcaatat gtggttcatc
     7261 aggtgcattt gctacagaaa aactggtatg cgattggtga aatgccatta aaaataattg
     7321 tcggccctga atgttatgca attgaccaat gattaattca cttttatcgt cccacgaaac
     7381 aatatgctgc
//
LOCUS       DAJTNA010000186         7257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTNA010000186 DAJTNA010000000
VERSION     DAJTNA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.188"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..207)
                     /locus_tag="OO161_003448"
     CDS             complement(<1..207)
                     /locus_tag="OO161_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5464391.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRRLTVK"
     gene            complement(209..1446)
                     /locus_tag="OO161_003449"
                     /pseudo
     CDS             complement(209..1446)
                     /locus_tag="OO161_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            complement(1443..1799)
                     /locus_tag="OO161_003450"
     CDS             complement(1443..1799)
                     /locus_tag="OO161_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464392.1"
                     /translation="MQSRSLLLDTGTWDILLDDTGNLAITDNPHAVAQDVACACSTFL
                     GECWYDSTAGIPYWPRILGHWPGTQLVNATLQQEALKLPTVSAAVCQVTADQTRTVTG
                     VLRITDTNNDIFTVLL"
     gene            complement(1812..2489)
                     /locus_tag="OO161_003451"
     CDS             complement(1812..2489)
                     /locus_tag="OO161_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5464393.1"
                     /translation="MAQPLSNPTDVNSEINAQDFMLRQFLGRHAFITLGRVVAVEEGF
                     IEARPMVMGVAADGSPVEHEVIYNIPVWRLQGGGNAVIIPPHVGDIGFLAICDRDISA
                     VKATRQAAMPGSKRTHNYADAIWLGGVLNGDPVQFVVFDDNQIRIVSPWKVEISAPEG
                     VINAPKSFTVNSPQIALNGDTAVSKGLDVTGQSRLSGGSNIGGIDFGNHVHGGVESGG
                     STTQGSR"
     gene            complement(2470..3339)
                     /locus_tag="OO161_003452"
     CDS             complement(2470..3339)
                     /locus_tag="OO161_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464394.1"
                     /translation="MSYRQRKITVEFTLSDGRTFGNGQGNMLTITDASCFASIAVYGG
                     VAGTQITLYIWGMSPAHMTNLSWRGVWRQEQSTANKMRLWADGRLIFEGDITDAYADY
                     NQAPDIPLILTGQIHFNLRNQPAADFSAKGDVAVADIIRALASTVGLGFENQGVSRSL
                     SDPHFSGNVVQQMLDVASAADINIDLGNVEKVTIWPKGQNRNIPPVLISPDHGLTGYP
                     VYTMTGLSATTIFCPDLFTGRPAHLESSLPDMTGDYTITGVIHTITSRTVGGPWSSNC
                     TMMRAEENGTTTQ"
     gene            complement(3336..3638)
                     /locus_tag="OO161_003453"
     CDS             complement(3336..3638)
                     /locus_tag="OO161_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464395.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEELI"
     gene            complement(3638..4348)
                     /locus_tag="OO161_003454"
     CDS             complement(3638..4348)
                     /locus_tag="OO161_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464396.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGCVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FTVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     ETQPSDADRRSATNRGIT"
     gene            complement(4345..6366)
                     /locus_tag="OO161_003455"
     CDS             complement(4345..6366)
                     /locus_tag="OO161_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5464397.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRLANAKAAFR
                     AGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMRKLAVIFPRLTKSEQTAYGNALGF
                     SYAGQQFLGSGHALKDVDEFTSRSQVTPGRTALARKLRKELVELDQTWSGIGMTISTA
                     LMPYALKLSHWLETLGDWMQQHPEEVNRFITTFLNDVESVASLANKAAGELGGWQNVI
                     ITLIGLKVASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDWLDSHGFF
                     WASDGTFFFNKKEMETYQTKLDAGEKPGNITRAQSPTVWQQGMLDTQASQATGREAAF
                     GESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPPGLLRSVA
                     ITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKLGGLIRFF
                     HGDLAKALAAYNCGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIRSLHPAPV
                     SQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(6350..6532)
                     /locus_tag="OO161_003456"
     CDS             complement(6350..6532)
                     /locus_tag="OO161_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5464398.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
     gene            complement(6574..6978)
                     /locus_tag="OO161_003457"
     CDS             complement(6574..6978)
                     /locus_tag="OO161_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464399.1"
                     /translation="MARKEKFITIEGQGRDKGKVFHLTEMSASQAEWWAMRAIMAMGR
                     GGVELPDDVRSLGMAALALEGLKALSKIPPDEARPLMDEMMDCVQFVPDPKKRSIRRP
                     LVEEDIEEIITRLNLRAEVFKLHVDFFATVVS"
BASE COUNT         1716 a         2096 c         1742 g         1703 t
ORIGIN      
        1 ctttaccgtc agccggcgac tgacacccac aatctttccc cagacatcaa gaccatactt
       61 ttctgcggta tcgatgttcc agataagacc ataaaaatca ctgataaaac tgtcggggga
      121 aagcgctgcg ttaaagctgt taatgagggc attgagtcgg gggctggcag catactgcgc
      181 aagcacagtg gcagccacat tctgcacgtt atgcctcctg gagtttcaca ctgatatccg
      241 acacatccag aaccggaatc tcatctatcc cgaaagtgac agccgttgtc catgacgaac
      301 cgtcacggct cacagtaagg cccagaatat caatatgttc cggatcggtt ttgtaaatgc
      361 cggcatagta gcgcccggca gagacaacag aggcaaccct tgcccgcaga ccaccatctg
      421 taccattaaa tgcgtccagc acggattgct gtacctgtcg ggtaatatct gaaggcaggt
      481 agtcactttt tttcagcgtc acactgacat gcagactgac gggtttgagt gtctgccagc
      541 tgattacgta ttcaggatac ggcggatcgt actccttatc tgcaacagtg aacgttgtgt
      601 tgccattcat atcaataccc ggcggagcct tacgccagat ggccgccgcg atatctgccg
      661 ggctgccgcc gtacacgcca acataaagcg aaccgggtgt taatggatac tgactgactc
      721 cggctttttg ttctgttttt ttcggattat gggggacgta aacatccacc acgttttcta
      781 ccgtggagag tatttcaccc cggatggctt ccagaatatt ccgggcatta cgggcaactg
      841 aattacgtcg gcggttttca aagtccgcac gggtttcctc atcgctgccc ggcacaccgg
      901 cacgggcgtt agtgacacct gaccagccag gtattgcctt ataaatttta ttcagcgtcc
      961 ccacaggaca gccgacgggg ccagtggaca aattcagaaa taccgcatca acctgccctg
     1021 atgcgccgat agtgacgtct gactgacata cttataaccg gcctcatcct gccccatact
     1081 gcccgccggg atcagcgtgc caaccagccc ggtacaggtc gctgttactg tcgtacctgt
     1141 ggccccacgg cgctccagga aataaatcct gcctattgca tcctgaaagc gaccactgga
     1201 aaagtcaggg ttgatctggt taacgatata cagcaactga tcgtttttat ccgcaataat
     1261 ggcgctttcg ctggatgcaa gttgtccctg cggactgctc agactggtac tcatggcccc
     1321 gcccagcgcc gccgaaaaat cagcaagcct gccgctcaga atatccgctt catccggcac
     1381 attcagcccg ctatccgaaa tctgtacagc gggcactgcg gtagaaaaag atttattttc
     1441 actcatagca gtaccgtaaa aatgtcatta ttggtatctg taatacgcag cacacctgtt
     1501 actgtccggg tttgatcagc tgtgacctgg cagacagcgg cgctcacggt cggcagttta
     1561 agtgcttcct gttgcagagt ggcattcacc agttgtgtgc cgggccagtg tccgaggatg
     1621 cgtggccagt aaggtatgcc agccgttgag tcgtaccagc actcccccag aaaagtactg
     1681 cacgcacacg ccacatcctg agctaccgca tggggattat cagtaatggc aagatttccg
     1741 gtatcatcaa gcaggatatc ccatgtcccg gtgtcaagaa gaagtgatcg tgactgcatg
     1801 ttttctcctg tttaccggga tccctgcgtg gttgagccgc cggactcaac gccgccgtga
     1861 acgtggtttc caaaatcaat acccccgatg ttcgaaccgc cggaaagcct ggactgcccg
     1921 gtaacgtcaa gccccttact gacggcggta tcgccgttca gcgcaatttg tggtgagtta
     1981 acggtgaagc ttttcggcgc attaatcacc ccctcaggcg cggatatctc cactttccag
     2041 ggagatacaa tccgtatctg gttgtcatca aataccacaa actggacggg atcaccgttg
     2101 agcacgccac ccagccagat ggcatcagca taattatggg tacgctttga tcccggcatc
     2161 gcagcctgac gcgtggcttt taccgcactg atatcccgat cgcagatggc aagaaaaccg
     2221 atatccccca catgtggcgg gataatcacc gcattaccgc ccccctgtag tcgccatacg
     2281 ggaatgttat aaatcacctc atgttcaacc ggggaaccgt ctgctgcaac gcccattacc
     2341 atcggtcggg cctcaataaa tccctcttcc acggctacca cccgtcccag agtgataaac
     2401 gcatgacgtc cgagaaactg ccgcagcata aagtcctgtg cattgatttc gctgttcacg
     2461 tctgtcgggt tactgagtgg ttgtgccatt ttcttcagcc ctcatcatgg tacagttgga
     2521 actccacgga ccgcccacgg ttcgcgaagt aatggtgtgt atcacccctg tgatcgtata
     2581 atcgcccgtc atatcaggca gtgacgattc cagatgcgct ggcctgccag taaagagatc
     2641 ggggcagaat atcgtggtgg cgctgagtcc ggtcatggtg taaaccggat agccagtcag
     2701 tccgtgatcc ggcgaaatca gtaccggcgg aatattccgg ttctgccctt tcggccagat
     2761 ggtgactttc tccacattcc ccagatcaat gttaatatcg gcggctgaag cgacatccag
     2821 catttgttgt acaacattac cggaaaaatg cgggtcagac agactgcggc tgaccccctg
     2881 gttttcaaat ccaagcccga cagttgacgc cagcgcacgg ataatatctg caaccgcaac
     2941 atcgcccttt gcgctgaaat ctgccgccgg ctgattacgc agattaaaat gaatctgtcc
     3001 tgtcagaatg agcggaatat ccggcgcctg gttgtagtcg gcgtatgcat cagtaatatc
     3061 accttcaaaa ataagccgcc cgtcggccca cagccgcatt ttatttgcgg tactctgctc
     3121 ctgtcgccac acaccgcgcc agcttaaatt cgtcatatgc gccggtgaca ttccccagat
     3181 atacagcgtg atttgtgtac cagccacccc tccatatacg gcaatactgg caaaacagct
     3241 tgcgtcggta atggtcagca tattgccctg gccattacca aacgttcgtc cgtctgaaag
     3301 agtgaactcc accgttattt tacgttgtct gtagctcata tcagttcctc aggtaacagc
     3361 cagtagagcc ggtaccggga tccgagcccc cgccagtcag ggtcactatt cccctccgtg
     3421 tcggtaaaaa acagatcccc ctgaaacggc aggtatccgt accggacaat ccggttatta
     3481 ttcagacaca gcacgccata caggcacggt tcaccattaa caatgatatc gatatacatc
     3541 cccgtagtac gctgattcag gcgaatggtg cagacctgag cgcccagtgt caccgtaaac
     3601 tgctgggctt taacgggaga taaaacaatt tccagcatca ggtgatcccc ctgttcgtgg
     3661 cgcttcgtct gtcagcgtct gacggttgtg tctccgacgc cgtaactggc tgcgttttaa
     3721 ccgatgcggc cccttttgct ttatcgttgt ccgtgggaga cgacttatcc gtacttccca
     3781 ctgacacctc tcctgtattc attacggtct gaaataccgc agtaaccgtc agtaaggtcg
     3841 gaccattatt acttcgcatt cggtagtcat atttcacaag gtcgcaggat gtccatgtct
     3901 tgtccggcgt ctcaatatcg tagagtcctg ccgtggtacg catcctttca agcgtttcca
     3961 gcacattcgt ccgcgaggtg gtggaaaaat ttgtcaggtt cgggacggat ccggaaaagg
     4021 ccgtccaccc ctctacagtg aaagtcacat gcagttccgg cgatctctgg attttattaa
     4081 aggtggtata agccccctgc tccacgggga cggtggaaac agaagcctcc gcgcccacct
     4141 caacgacaac aaaagaatcc ggggagaaag gtttgccacc cttctgatgg gcacctgccg
     4201 gatcattccg tgcgtaataa ataccgaatg acggtgccag tacactatta atgagtccca
     4261 ggacacagcc gccacgaact gcactcagta cgttactttc actgagcgaa aagttattca
     4321 gggaaatatt atcgaaagaa aaactcatcc tgttaccccg ctggaataaa ccgacacaag
     4381 cgccgaattc gtgatacgcc gacgtgcgtc atcggtaata cccttcacat tgtccgacgt
     4441 tgtggtgaca ttcagtgtcc cgatatgcgt ggtttccgtt acggtggact gcgatacagg
     4501 cgcaggatgg agcgaccgta tggccattgc cgcacccgga taaggcaggt tcgccagcac
     4561 ccgcggaata tagttgcggg tctcctccgg agcagcagcc agtcccttac gctgaacatt
     4621 tccctctcca cagttatatg ccgccagagc cttagccaga tcgccatgaa aaaaacgtat
     4681 caggccacca agttttctcg cggcggcatc agcggctttt tcgggatcaa aggcatcgtt
     4741 ccccctcaga ccaaattcac tggccgtctt cggcatgaac tgaaacagtc ccatcgcacc
     4801 agcaggtgag acggcaaact gattaccacg ggattcggtg atcgcaacgc tgcgcagcag
     4861 tccgggcggc aggttatatt tttcctccag ttgagacagt ttcggttgca gccagcctaa
     4921 cagggcttcc ccggtcttcg tcggtcgctg gcggttttgc atggcattac cgagtttttc
     4981 ctgcgtcgca cgcataccct gtagccagga ttcaccgaag gctgcttccc tcccggttgc
     5041 ctgagaagcc tgagtatcca gcatgccctg ctgccatact gtgggtgatt gtgcccgggt
     5101 gatgttgcca ggcttttctc cggcatccag ttttgtctga tacgtctcca tctctttctt
     5161 attgaaaaag aaagtcccgt ctgaagccca gaaaaaacca tgtgaatcca gccagtcctt
     5221 attttccctg ccaacaatgg atgtcagtaa tccgtcaaca accgggtaaa gcgccgttat
     5281 cgcaaaaaga aggcctccgg ggccactgag ggcttttgtc agcccccgca cccatgacgc
     5341 cactttcagc ccgatcagcg taataatgac attctgccag ccccccagtt ctccggcagc
     5401 cttattcgct agggaggcca ctgactcaac gtcattcaga aatgtggtga taaacctgtt
     5461 cacttcctcc ggatgttgct gcatccagtc accgagtgtc tccagccagt ggctgagctt
     5521 cagcgcgtac ggcatcaacg cggtgctaat cgtcatcccg atgccggacc aggtctgatc
     5581 gagctcaaca agctcttttc gtagcttacg ggccagcgct gtacgccccg gcgtaacctg
     5641 cgaacgggaa gtaaattcgt ccacatcttt cagcgcatga ccggagccaa gaaactgctg
     5701 tccggcataa ctgaagccca gcgcgttgcc gtaggcagtt tgttcggact ttgtcagtcg
     5761 cggaaaaata accgccagct tgcgcatgat ggcttcagtg ctgtcagtat ttaaatcaac
     5821 actaatgccc gccctggctg caacctgaaa caaatcctgt aacacaggat caaaggacct
     5881 tccggctctg aaggccgctt ttgcatttgc aagccgggaa aatgccccag acatttcccc
     5941 ttcatcaaca ttatttgccc gcgcagcccg taaccatccg tccagatttc tggccttcat
     6001 gccaaaagca tcggattcca cggacagacg gttcagatca cgggcaaaac ctgttaccag
     6061 gttcttcagc ccccccagcg tcagactgac acccgcaaga gccagaatct cgctacggat
     6121 tcctgaaaaa aaacgggacg cccgtttccc ggcatcttcc attcgacggg ccgttttatc
     6181 agcatctttt ccggtttttt ccagtgattc actggttttc ttctgtccct gactgaatgt
     6241 gtcagcgaca ccttccatca ccgtcgtgag ccggttaagg gcagcatgta ctgcctgttc
     6301 cccggcagta aagtccctgt catcaatccc gagggacaga acaagctcat caagtacctg
     6361 cgccatttct gttctcctgt atcacctgtt cgttatgggc atccacatga attatttcca
     6421 gcagatccca taagtcctgc acaccaagga cagaatccag ttcagctttt gaagccttac
     6481 cggaggagat aacggtcgca atggtacggg gaacattaac gtaatccacc accccgaacg
     6541 gtctgtcggg gtcgagataa cgcgggggga tatctagctg acgacggtag cgaaaaaatc
     6601 cacatgcagc ttaaagacct ccgcacgcag attaagacgg gtaatgattt cttcaatatc
     6661 ctcttcaaca agcggtctcc ggatgctgcg ttttttcgga tcgggaacaa actgcacaca
     6721 gtccatcatt tcatccatca acggacgcgc ctcatcaggc ggaattttcg ataatgcttt
     6781 cagcccttca agggcaagcg cagccatccc cagactacga acatcatccg gtaactccac
     6841 gccaccacgc cccattgcca taatggcacg catcgcccac cattccgcct gtgaggcaga
     6901 catttcggta agatgaaata ccttgccctt atcccgtccc tgaccttcaa tagtgataaa
     6961 tttctcttta cgggccatca gttaaaaacc tccggagtaa tggtttccca ctcaataacc
     7021 gcctgccctg gctgcaacgt acgagctgca tcaggcagcg ctttccattg tttgagtacg
     7081 ccattcacgc aggtatattt acggccaatc gccggaagca ggacgacagc gttacaacgg
     7141 aatacagccc gactggcccg gatgtggttg accaggtatc aaaaatatcc cggctggggg
     7201 aatccggcat gatatgaaac gtctggataa tgttgctgta cacaaatccc gcggaca
//
LOCUS       DAJTNA010000187         7228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTNA010000187 DAJTNA010000000
VERSION     DAJTNA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.189"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(36..1004)
                     /locus_tag="OO161_003458"
     CDS             complement(36..1004)
                     /locus_tag="OO161_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR5464400.1"
                     /translation="MKDQITHLPDNADRTMAKQKFKLTNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVP
                     LRCPDYTSVSKRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRVASADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDSQVAEAMALVRALNKMTKAGMPESVRIA"
     gene            1043..1687
                     /gene="traX"
                     /locus_tag="OO161_003459"
     CDS             1043..1687
                     /gene="traX"
                     /locus_tag="OO161_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5464401.1"
                     /translation="MIKTVALVLMVLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLN
                     LSRHAHIRQPAINRLWGWGIIAQFAYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGW
                     RTAAAILLMALWGPLSGTSYGIAGLLMLAVSNRLYRAEDRAERLALVACLLAVIPALN
                     LATSDAAAVAGLVMTVLTVGLVLCAGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            1742..2302
                     /locus_tag="OO161_003460"
     CDS             1742..2302
                     /locus_tag="OO161_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5464402.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWSGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            2433..2645
                     /locus_tag="OO161_003461"
     CDS             2433..2645
                     /locus_tag="OO161_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5464403.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            2889..3350
                     /locus_tag="OO161_003462"
     CDS             2889..3350
                     /locus_tag="OO161_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5464404.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            3396..3605
                     /gene="hha"
                     /locus_tag="OO161_003463"
     CDS             3396..3605
                     /gene="hha"
                     /locus_tag="OO161_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5464405.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            3644..4234
                     /locus_tag="OO161_003464"
     CDS             3644..4234
                     /locus_tag="OO161_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5464406.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            4474..4734
                     /gene="repA"
                     /locus_tag="OO161_003465"
     CDS             4474..4734
                     /gene="repA"
                     /locus_tag="OO161_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5464407.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            4958..5032
                     /gene="tap"
                     /locus_tag="OO161_003466"
     CDS             4958..5032
                     /gene="tap"
                     /locus_tag="OO161_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5464408.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            5025..5882
                     /gene="repA"
                     /locus_tag="OO161_003467"
     CDS             5025..5882
                     /gene="repA"
                     /locus_tag="OO161_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5464409.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(6866..>7228)
                     /locus_tag="OO161_003468"
     CDS             complement(6866..>7228)
                     /locus_tag="OO161_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000487120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5464410.1"
                     /translation="GQGYRLVFQFPIREQAAAFLGVVPVEKYSGTSVRGCPKLSKIGP
                     PEIRAKLYLASLCGLRFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVLKTQK
                     PFDVGYLSSYARSACKAA"
BASE COUNT         1828 a         1777 c         1924 g         1699 t
ORIGIN      
        1 tttcgggtaa gtctcccccg tagcgggttg tgttttcagg caatacgcac gctttcaggc
       61 atacctgctt tcgtcatttt gttcagcgct cgtaccaggg ccatagcctc cgcaacctga
      121 ctatcgtagt cacgcagcgt cagtgaaccc ccgaacagct gttttaccct gtacatcgcc
      181 gtttccgcta tcgagcgacg gttgtaatct gttgtccatt tccaccgcgc attactcccg
      241 gtcattcgct gattagccac tgcacggtta cggtctgcat attcaccggg ccagtaaccc
      301 gcaccttttc ggggagggat aagcgcgctg attttcttac gccgcagttc atcgtgacag
      361 agccgggtgt cgtaagcgcc gtctgccgat gctaccctga tttttctgtg agtctgccgg
      421 ataagacccg ggaaggcttc tgagtccgtc acattgttca gcgacaggtc agcgcagatg
      481 atttcatgtg ttttactgtc aacggcgaga tgcagcttac gccagatacg gcggcgttcc
      541 tggccatgct ttttgacttt ccactcgcct tcaccgaaga ccttcagccc ggtggaatca
      601 attaccaggt gtgcgatttc accccgggtg ggcgttttga aactgacatt aaccgacttt
      661 gcgcgcttgc tgacactggt gtaatccggg cagcgcaacg gaacattcat cagtgtaaaa
      721 atggaatcaa taaaaccctg tgcagcccgc agggtcaacc tgaacacgcg tttaatgacg
      781 agaacggtgg tgatggcgag atcagaatag cgctgaggtc ttccccgtga tgaaggcgtt
      841 gccgactcat accaggcctg aatagcttca tcatccagcc agaaagttat ggagccacgg
      901 ttgatgaggg ctttattgta ggtgggccag ttggtgagtt tgaacttttg ctttgccatg
      961 gtacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc aaaagttcga
     1021 tttattcaac aaatcccggg atatcatcaa aacggtggca ctggtgctga tggtactgga
     1081 ccacatcaat ctgatattcc agctgaagca ggaatggatg tttctggccg ggcgaggagc
     1141 ctttccgctt tttgccctgg tgtgggggct gaatctgtcc cgtcatgcgc atatccggca
     1201 accggccatt aaccggctgt ggggatgggg aattattgcc cagttcgcat attatctggc
     1261 aggctttccc tggtatgagg ggaatatcct gtttgccttt gcagtggcgg cacaggtgct
     1321 gacgtggtgt gaaacgcgca gtgggtggcg tacagcagcg gccatactcc tgatggccct
     1381 gtgggggcct ttgtccggca ccagttacgg tattgccggg ttgctgatgc tggcagtcag
     1441 caaccggctg taccgggcgg aagacagagc ggaacgtctg gcgctggtcg cctgcctgct
     1501 ggctgttatt ccggcgctta accttgccac gagtgatgca gcggcggtgg ccggtcttgt
     1561 gatgacggtg ctgaccgttg gtctggtgtt gtgtgccgga aaatcattac cccgtttctg
     1621 gcccggagac tttttcccga cgttctatgc ctgtcatctg gctgtgctgg gcgttctggc
     1681 gctgtgacgg gtgtggtatc tttggccgca agaggatgat tcgtcagagg cagaacacag
     1741 catgacagag cagaagcgac cggtactgac actgaagcgg aagacagagg gaacagcgac
     1801 tgtccgcagc cggaaaacca tcatcaatgt caccacgcca ccaaagtgga aggtgaaaaa
     1861 gcagaagctg gccgagaaag cggcccgtga agcagagctg gcggcaaaaa aagcgcaggc
     1921 cagacaggcg ctgtccattt atctgaacct gcccacgctg gatgaggctg tgaataccct
     1981 gaagccctgg tggtcgggat tatttgacgg tgacacgccc cggcttctgg cctgcggtat
     2041 ccgggacgtg ttactggaag acgtggcgca gcggaatatc ccgctctcgc ataaaaaact
     2101 gcgcagggcg ctgaaggcca tcacccgttc agaaagctat ctgtgtgcca tgaaagccgg
     2161 tgcctgccgg tatgacacgg aagggtatgt gacggagcat atttctcagg aggaggaagc
     2221 gtatgcggga gcgcgtctgg cgaaaattcg tcgccagaac cggataaagg cagaacttca
     2281 ggccgtgctt gatgagaaat aaaaaaagcc tcccccagag agaggaggca gggaaataag
     2341 gcttaaaagg aaataacttc cgcttccgaa aggaagccag tccactgaat tatttatcac
     2401 aataatactg acaactaaag ggggacaaga aaatgaatgg attcagaaac agttcacgga
     2461 acggccaggt ctggcgttac cagcgtgccg gcgggcgggc cgttattctg gaagtcagtg
     2521 gacgctggat ggaagcggca gaagcatggc gacgggctgc ctgtgttgcg cctaggacag
     2581 actggcagca gtttgcccgg aaaagggctg aacattgcca ccggcgctgc cggggaaggg
     2641 ggtaacgaaa aaagcagcct tcattactcc aggagtcaga aggctgaaag gagtttatga
     2701 gatttgcttc ttaatatccg gtcgggcagt atgaccggac agttctgagt agcacagagt
     2761 cttttccgca ttaagtcgat tttttatatt ttgtgattaa tttcacaaaa taaggtgtta
     2821 ttcaatgtgt gctgcactat tcaggggggc tgaaatacag gtgccattta tctgatatgg
     2881 agaataacat gaggaaatat attccactgg tattatttat cttttcatgg ccggtattgt
     2941 ctgcagatat tcatggacgg gttgttcgtg tactggacgg tgataccatc gaggtcatgg
     3001 actcactgaa ggcggtgcgg atcaggctgg taaatattga tgcaccggaa aagaaacagg
     3061 attatggccg gtggtcaaca gacatgatga aatcactggt cgcaggaaaa acggttaccg
     3121 tcacatattt tcagcgagat cgttatggtc gtattctcgg gcaggtttat gcaccagacg
     3181 gaatgaatat taatcagttt atggtccggg ccggggctgc ctgggtatat gagcagtaca
     3241 acacagatcc cgtgctacct gtccttcaga acgaagccag acaacaaaaa cgtgggctct
     3301 ggtcagatgc tgatccggtt cccccctgga tatggaggca ccgtaaataa agcgatggca
     3361 atcctatcat tcatctgttc tcagcgggaa taaaaatggc aaaaacaaaa caggaatggc
     3421 tgtatcagtt acggcgatgt tcatcagtca atacgctgga aaaaattatt cataaaaata
     3481 gagacagttt atcaaacagt gaacgtgaat catttaattc agcagcagac caccggctag
     3541 cggaattaat caccggaaag ttatatgaca ggataccaaa agagatatgg aaatatgtaa
     3601 ggtaataatt cagcaataat taattgccag gagcagataa ataatgaagt tgattatttt
     3661 tattctcatt gttctgatta ttgcagcgct acttatccgg attatcctca gaagtgtaaa
     3721 tcagcattct cctttgctga tgcaattgca tgcggcaggt atacggaccg gtgatgcaga
     3781 gcgaatactg tccagcggtg aatactggca acgtcagaag acgctgctga cagaaaggga
     3841 agtcagtttt atgaaaggac tgttcagaat tgtggatatg aagcggtggt atctgtgtcc
     3901 gcaggtacgg gtcgcggata tcgtccagct gaacgggaat atccggccac gatcacgcca
     3961 gtggtggcag ttattcagga tggtgtctca gtggcatgtt gatgtggtta tcgttgagct
     4021 gcgttcgttc agtattgttg cagcagtaga gctggatgat gccagccatt tacgaccgga
     4081 acgcagacgc cgggatattc ttctggaaga ggttctgagg caggctggta ttccgttgct
     4141 cagaagccac gatgccagaa aactgctgca gatgaccgga gaatggctga atacaacagg
     4201 ggctgatcag cagtcaccgg aacatcgtag ctgacgcctt cgcgttgctc agttgtccaa
     4261 ccccggaaac gggaaaaagc aagttttcct tgctcccggc gtttccataa ctgaaaacca
     4321 tactatttca cagtttaaat cacattaaac gacagtaatc cccgttgatt tgtgcgccaa
     4381 cacagatctt cgccacaatt ctcaagtcgc tgatttcaaa aaactgtagt atcctctgcg
     4441 aaacgatccc tgtttgagta ttgaggaggc gagatgtcgc agacagaaaa tgcagtgact
     4501 tcctcattga gtcaaaagcg gtttgtgcgc agaggtaagc ctatgactga ctctgagaaa
     4561 caaatggccg ctgttgcaag aaaacgtctt acacacaaag agataaaagt ttttgtcaaa
     4621 aatcctctga aagatctcat ggttgagtac tgcgagagag aggggataac acaggctcag
     4681 ttcgttgaga aaatcatcaa agatgaactg cagagactgg atatactaaa gtaaagactt
     4741 tactttgtgg cgtagcatgc tagattactg atcgtttaag gaattttatg gctggccacg
     4801 ccataaggtg gcagggaact ggttctgatg tagatgtaca ggagccagaa aagcaaaaac
     4861 cccgataatc ttctttaact ttggcgagtc agaaagatta ccggggccca cttaaaccgt
     4921 atagccaaca atcaagctat gcggggagta tagttatatg cccggaaaag ttcaagactt
     4981 ctttctgtgc tcgctccttc tgcgcattgt aagtgcagga tggtgtgact gatcttcaac
     5041 aaacttatta ccgccaggta aagaacccga atccggtgtt cacaccccgt gaaggtgccc
     5101 ggacgcttcc gttctgcggg aaactgatgg aaaaggcggt gggcttcacc tcccgttttg
     5161 atttcgccat tcatgtggca catgcccgtt cgcttggtct gcgtcggcgc atgccaccgg
     5221 tactacgtcg ccgggccatt gatgcgctgc tgcaggggct gtgttttcac tatgacccac
     5281 tggccaaccg cgtccagtgt tccatcacca cgctggccat tgagtgcgga ctggcgacgg
     5341 agtctgctgc cggaaaactc tccatcaccc gggccacccg ggccctgacg ttccttgcag
     5401 agctgggact gattacctac cagacggaat atgacccgct tatcgggtgc tacattccga
     5461 ccgatatcac gttcacatct gcactgtttg ctgccctcga tgtatcagag gaggcagtgg
     5521 ccgccgcgcg ccgcagtcgt gtggaatggg aaaacagaca gcgcaaaaag caggggctgg
     5581 ataccctggg tatggatgaa ctgatggcga aagcctggcg ttttgttcgt gagcgtttcc
     5641 gcagttacca gacagagctt aagtcccgtg gaatgaagcg tgcccgtgcg cgtcgtgatg
     5701 cggacaggca gcgtcaggat attgtcaccc tggtgaaacg gcaactgacg cgtgaaatct
     5761 cggaagggcg cttcactgcc agtcgtgagg cggtaaaacg tgaagtggag cgtcgtgtga
     5821 aggagcgcat gattctgtca cgtaaccgta attacagccg gctggccaca gcttccccct
     5881 gaaagtgacc tcctcagaat aatccggcac gcgccggagg cattcgcacg cctgaagccc
     5941 gccagtgcac aaaaaatcag cgctacatac aaaaaacaac ctcatcatcc accttctggt
     6001 acatccggtc ccccctgttt ttgatacaaa acacgcctca cagacgggga attttgctta
     6061 tccacattaa actgcaaggg acttcaccat aaggttacaa ccgttcacgt cataaagcgc
     6121 cagccgtcag ccctacaggg tgcaatgtat cttttaaaca cctgtttgta tctcctttaa
     6181 actacttaat tacattcatt taaaaagaaa acctgttcac tgcctgtcct gtggacagac
     6241 agatatgcac ctcccaccgc aagcggcggg ccccgaccga agctggttta gttacaacat
     6301 acaaacataa ccccctaaaa aacccaagtc caacgcagaa ctgaaaccac aaagcccctc
     6361 cctcataact gaaaagcggc cccgccccgg cccgaagggc cggaacagag tcgcttttaa
     6421 ttatgaatgt tgtaactata ttatcatcgc tgtcagtcct ctgactggac gtatcgagta
     6481 cacgcccgta agcggccctc acggcccgct aacacggaga tacgccccga ctgcgggtaa
     6541 acctttgttg ggctccgacc gcgcacagaa gctatgtcat ggctgaaaac gggtatggct
     6601 tggcagttat cgtgttctgt cgagaaaaat cgcttgaact ccaacgtaca gccccctaag
     6661 tactgccagc aagctgtcag cactaagtcc ccgatacagc gcggcatagc cgcgctaacg
     6721 gggttaaaag ggcttaccta tacttcatca tcgctgaaag cccgcatggc ctcgcagggc
     6781 aggaatggga taggtgaaac ctatcaatac gtaccggctt acgccgggct tcggcggtgt
     6841 tactcctgta ccatatgtaa ctacatcatg ccgccttaca tgcgctacgc gcatatgaag
     6901 acaaatagcc cacatcaaac ggtttttggg tcttcagtac gccatagcac cagtgcacga
     6961 gttttctcat cagcgcacca atggcacaca ttttggcctt acctctcagg cataatcgct
     7021 catacatcgc tttcatcaat gggttaaatc tcagaccaca caacgaagcc agatacagtt
     7081 tcgcccggat ttccggcgga ccaatttttg acagtttcgg acaccctctg accgaggtgc
     7141 cggaatattt ttcaacggga acgacaccta aaaatgcagc cgcctgttca cgtataggaa
     7201 attgaaaaac taagcgatac ccctgtcc
//
LOCUS       DAJTNA010000188         7182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTNA010000188 DAJTNA010000000
VERSION     DAJTNA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.190"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..1240
                     /locus_tag="OO161_003469"
     CDS             <1..1240
                     /locus_tag="OO161_003469"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HCR5464411.1"
                     /translation="AEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGY
                     GDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLV
                     LNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAE
                     LNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLS
                     GAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTS
                     YNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSY
                     LGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDI
                     EMRADGEVAVDAGVDTQWGH"
     gene            1240..2037
                     /locus_tag="OO161_003470"
                     /pseudo
     CDS             1240..2037
                     /locus_tag="OO161_003470"
                     /inference="COORDINATES: protein motif:HMM:NF015188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2197..3030
                     /locus_tag="OO161_003471"
                     /pseudo
     CDS             2197..3030
                     /locus_tag="OO161_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3158..3880)
                     /gene="ubiG"
                     /locus_tag="OO161_003472"
     CDS             complement(3158..3880)
                     /gene="ubiG"
                     /locus_tag="OO161_003472"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HCR5464412.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHMQNK"
     gene            4027..6654
                     /gene="gyrA"
                     /locus_tag="OO161_003473"
     CDS             4027..6654
                     /gene="gyrA"
                     /locus_tag="OO161_003473"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5464413.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKEIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLGEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSVA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6803..>7182
                     /locus_tag="OO161_003474"
     CDS             6803..>7182
                     /locus_tag="OO161_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HCR5464414.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLD"
BASE COUNT         1725 a         1767 c         2052 g         1638 t
ORIGIN      
        1 tgccgaaggt tatggcgggg cgatatatac caatagcgcg acggctccct atcttattga
       61 tatttctgtt gatgacagct acagccagaa cggcggcgtg ttagtggatg agaacaacag
      121 cgcagcaggc tatggagatg gtccttccac tgcggcgggt ggctttatgt atctcggctt
      181 aagtgaagtt acctttgata ttgccgacgg aaaaacgctg gttatcggca atacagagaa
      241 tgacggagct gttgactcta ttgctggtac cgggttgatc accaaaacag gttccggcga
      301 tctggtactt aatgcagata acaatgactt tactggcgag atgcagattg aaaacggtga
      361 agttaccctg ggccgcagca actccctgat gaatgtcggc gatacgcatt gccaggacga
      421 tccgcaagac tgctacggtc tgacgatagg gagtattgat aagtaccaga atcaggcaga
      481 gctaaacgtt ggctccaccc aacaaacctt tgtgcactca ttgacgggct ttcagaatgg
      541 cactttaaat atcgatgctg gtggcaatgt tactgttaat caaggtagtt ttgctggcac
      601 catcgaaggt gctggtcagc tcaccattgc gcaaaacggc agctacgtgc tgtcgggggc
      661 gcagtcgatg gcgctaaccg gcgatatagt cgttgatgat ggtgcggtgc tttcgctgga
      721 aggcgacgcg gcagatctcg ccgctctcca ggacgatccg cagtcgatcg tgttaaacgg
      781 cggtgtgctc gatctctctg atttctccac ctggcagagc ggcacatcat acaatgatgg
      841 ccttgaagtc agtggcagca gcggaacggt tatcggcagt caggatgtgg tagatcttgc
      901 aggtggcgac aatttgcata tcggcggcga cgggaaagat ggcgtctacg tggtggtcga
      961 tgcgagcgac gggcaggtaa gtctggcaaa caataatagt tatttgggca caacacaaat
     1021 cgcctccggt acgctgatgg tgagcgacaa ctcgcagctt ggagataccc actataaccg
     1081 ccaggttatc tttaccgata agcaacaaga aagcgtgatg gagattactg ccaatgtcga
     1141 tactcgctct acgacgactg agcatgggcg tgatattgaa atgcgcgccg acggtgaagt
     1201 ggcagttgat gcgggggtag acacgcagtg gggccactga tggctgacag cagcgggcag
     1261 catcaggatg agggtagcac atttactaaa acgggggcgg gtacgctgga gctgaccgcc
     1321 agcggtacaa cgcagtcggc agtacgtgtc gaagaaggca ccctgaaagg cgatgttgcg
     1381 gatatctttc cttatgcttc gtcactgtgg gtcggtgatg gggcgacgtt cgttactggc
     1441 gcggatcagg atattcagtc tatcgatgct acttccagcg gcactatcga tatcagcgat
     1501 ggtacggttt tgcgtctgac cgggcaggat acttccgtcg cccttaatgc ctcattgttt
     1561 aacggcgatg ggacgctggt gaatgctacc gatggcgtga cgttgacagg tgagcttaat
     1621 accaaccttg aaactgacag cctgacctat cttgccgacg tgacggttaa tggtgatctg
     1681 accaatacgt ccggtgcggt tagcctgcaa aatggcgtcg ctggcgatac gctaacggta
     1741 aacggtgatt ataccggcgg cggtacgcta ctgttcgaca gcgaattaaa cggcgatgac
     1801 tcggtaagcg atcaattggt gatgaacggt aatactgctg gcaacacaac tgtggtggtt
     1861 aactccatta cagggattgg tgagccgaca tcgacaggca ttaaagtggt tgatttcgca
     1921 gctgatccca cgcagtttca aaacaatgca cagttcagtc tggctggcag tggctacgtc
     1981 aacatgggag catatgacta cacgctagtg gaagataaca acgactggta tctgcgatcg
     2041 caagaagtaa cgccgccatc gccatctgat ccagacccgg acccaacacc cgatcctgac
     2101 ccaacacccg atccaatccc cgcttaccag ccggtgctga atgccaaagt tggcggttat
     2161 ctcaataacc tgcgggcggc aaatcaggcg tttatgatgg agcgacgcga tcacgctggt
     2221 ggcgatggtc agacgctgaa tttacgtgtt atcggcggac gttatcatta cacggcggca
     2281 gggcaactgg ctcagcagga agacacttct acggtgcagc ttagcggcgg cttgtttagc
     2341 gggcgctggg gcacggatgg cgagtggatg cttggggcgg ttggtggcta cagtgataat
     2401 cagggcgaca gccgctcgaa tatgaccgga actcgcgccg ataaccagaa ccacggttat
     2461 gccgttgggc tgacctcaag ctggtatcag cacggtaatt agaagcaggg ggcctggctg
     2521 gatagctggc tgcaatacgc gtggtttaac aatgatgttt ccgaacatga agatggcgtg
     2581 gatcgttacc attcgtcggg gattatcgcc tcgctggaag ccgggtatca gtggttaccg
     2641 gggcgtggtg tggtgattga accgcaggcg caggtgattt atcaggacgt gcagcaggat
     2701 gattttaccg ccgctaacca tgcgcgcgtg tcacaatcgc agggggatga tattcagacg
     2761 cggctgggtt tacacagcga atggcgtacc gcggttggtg tcacaccaac attagatctg
     2821 aattattatc acgatcccca tgcgacggaa attgaagaag atggcagcac tatcagtgac
     2881 gatgcggtga agcgacgggg tgaaataaaa gtgggagtca cgggcaatac cagtcagcga
     2941 gtgtccctgc gcggcagcgt ggcgtggcag aaagggagtg atgattttgc ccagacggca
     3001 gggtttttgt cgatgacggt gaaatggtaa tcactgtcta gtcggcacgg ttgccggatg
     3061 cggcgtaaac gccttatccg gcctacgggg agcatttgta ggcctgataa gacgcggcaa
     3121 gcgtcgcatc aggcattggc tcggccaaac aacaacctca cttattctgc atgtgcagca
     3181 tatagttcac atccacgccg gggccgagtt taaaagtatt agtgagcggg ttgtaatgta
     3241 gcccggttat atgtcgctct ttaaggcttg tctgatctac ccagccaagc agttctgccg
     3301 gtttaataaa cttcttcaca tcatgcgtgc ctttgggcac catgcgcaaa atatattccg
     3361 cgccaaccac cgccatcagc catgacttgc cgttgcggtt aagcgtcgag aaaaagacat
     3421 cgccgcctgg tttcaccagt tgcgcacagg ctctgaccac tgactgcgga tcggggacgt
     3481 gctccagcat ctccatgcag gtcaccacat catactgccc ggcatgtttt gccgcgtgct
     3541 cttccacggt ttcctgcaca taatccacct gaatgccgct ttccagcgcg tgcagttttg
     3601 ccacctgcaa tggctcaaag cccatatcca gaccggtcac cgtcgcgcct tcgcgcgcca
     3661 tactctcggc cagaatgccg ccgccacaac cgacatcgag cacctttttg ccaaataaac
     3721 cgccagcacg ctcggcaata tagcccagac gcagcgggtt aatgcggtgc aacggtttga
     3781 actcaccttc aagatcccac cagcgggagg cgacggcttc aaatttagcg atctcttcgt
     3841 ggtctacgtt atggttttcc ggcgattttt cggcattcat tggcacttct actccgtaat
     3901 tggcaagaca aacgagtata tcaggcattg gatgtgaata aagcgtatag gtttacctca
     3961 aactgcgcgg ctgtgttata atttgcgacc tttgaatccg ggatacagta gagggatagc
     4021 ggttagatga gcgaccttgc gagagaaatt acaccggtca acattgagga agagctgaag
     4081 agctcctatc tggattatgc gatgtcggtc attgttggcc gtgcgctgcc agatgtccga
     4141 gatggcctga agccggtaca ccgtcgcgta ctttacgcca tgaacgtact aggcaatgac
     4201 tggaacaaag cctataaaaa atctgcccgt gtcgttggtg acgtaatcgg taaataccat
     4261 ccccatggtg acttggcggt ttatgacacg atcgtccgta tggcgcagcc attctcgctg
     4321 cgttacatgc tggtagacgg tcagggtaac ttcggttcca tcgacggcga ctctgcggcg
     4381 gcaatgcgtt atacggaaat ccgtctggcg aaaattgccc atgaactgat ggccgatctc
     4441 gaaaaagaga cggtcgattt cgttgataac tatgacggca cggaaaaaat tcccgacgtc
     4501 atgccaacca aaattcctaa cctgctggtg aacggttctt ccggtatcgc cgtaggtatg
     4561 gcaaccaaca tcccgccgca caacctgacg gaagtcatca acggttgtct ggcgtatatc
     4621 gatgatgaag acatcagcat tgaagggctg atggaacaca tcccggggcc ggacttcccg
     4681 acggcggcaa tcattaacgg tcgtcgcggt attgaagaag cttaccgtac cggtcgcggc
     4741 aaggtgtata tccgcgctcg cgcagaagtg gaagttgacg ccaaaaccgg tcgtgaaacc
     4801 attatcgtcc acgaaattcc gtatcaggta aacaaagcgc gcctgatcga gaagattgcg
     4861 gaactggtaa aagaaaaacg cgtggaaggc atcagcgcgc tgcgtgacga gtctgacaaa
     4921 gacggtatgc gcatcgtgat tgaagtgaaa cgcgatgcgg tcggtgaagt tgtgctcaac
     4981 aacctctact cccagaccca gttgcaggtt tctttcggta tcaacatggt ggcattgcac
     5041 catggtcagc cgaagatcat gaacctgaaa gaaatcatcg cggcgtttgt tcgtcaccgc
     5101 cgtgaagtgg tgacccgtcg tactattttc gaactgcgta aagctcgcga tcgtgctcat
     5161 atccttgaag cattagccgt ggcgctggcg aacatcgacc cgatcatcga actgatccgt
     5221 catgcgccga cgcctgcaga agcgaaaact gcgctggttg ctaatccgtg gcagctgggc
     5281 aacgttgccg cgatgctcga acgtgctggc gacgatgctg cgcgtccgga atggctggag
     5341 ccagagttcg gcgtgcgtga tggtctgtac tacctgaccg aacagcaagc tcaggcgatt
     5401 ctggatctgc gtttgcagaa actgaccggc cttgagcacg aaaaactgct tggcgaatac
     5461 aaagagctgc tggatcagat cgcggaactg ttgcgtattc ttggtagcgc cgatcgtttg
     5521 atggaagtga tccgtgaaga gctggagctg gttcgtgaac agttcggtga caaacgtcgt
     5581 actgaaatca ccgccaacag cgcagacatc aacctggaag atctgatcac tcaggaagat
     5641 gtggtagtga cgctctctca ccagggctac gttaagtatc agccgctttc tgaatacgaa
     5701 gcgcagcgtc gtggcgggaa aggtaaatct gccgcacgta ttaaagaaga agactttatc
     5761 gaccgactgc tggtggcgaa cactcacgac catattctgt gcttctccag ccgtggtcgc
     5821 gtctattcga tgaaagttta tcagttgccg gaagccactc gtggcgcgcg cggtcgtccg
     5881 atcgtcaacc tgctgccgct ggagcaggac gaacgtatca ctgcgatcct gccagtgacc
     5941 gagtttgaag aaggcgtgaa agtcttcatg gcgaccgcta acggtaccgt gaagaaaact
     6001 gtcctcaccg agttcaaccg tctgcgtacc gccggtaaag tggcgatcaa actggttgac
     6061 ggcgatgagc tgatcggcgt tgacctgacc agcggcgaag acgaagtaat gctgttctcc
     6121 gctgaaggta aagtggtgcg ctttaaagag tcttctgtcc gtgcgatggg ctgcaacacc
     6181 accggtgttc gcggtattcg cttaggtgaa ggcgataaag tcgtctctct gatcgtgcct
     6241 cgtggcgatg gcgcaatcct caccgcaacg caaaacggtt acggtaaacg taccgcagtg
     6301 gcggaatacc caaccaagtc gcgtgcgacg aaaggggtta tctccatcaa ggttaccgaa
     6361 cgtaacggtt tagttgttgg cgcggtacag gtagatgact gcgaccagat catgatgatc
     6421 accgatgccg gcacgctggt acgtactcgc gtttcagaaa tcagcatcgt gggccgtaac
     6481 acccagggcg tgatcctcat ccgtactgcg gaagatgaaa acgtagtggg tctgcaacgt
     6541 gttgctgaac cggttgacga ggaagatctc gataccatcg acggcagtgt cgcggaaggg
     6601 gacgatgaaa tcgctccgga agtggacgtt gacgacgagc cagaagaaga ataattttac
     6661 ttcttcatgc caaaagggag ctatctccct tgtttgaatt gaaaagtcca ggctgcaaag
     6721 tctgggcttt tgtcgtatta gggcacggta aagtttggct gtgcccgtaa aaaatggctg
     6781 gctatacaca aggaatgtgg caatgagtgg tgaaaaaaag gcgaaaggct ggcggttcta
     6841 tggtcttgta ggttttggtg caatagtact gctttccgct ggcgtctggg cgttgcaata
     6901 tgctggcagt gggccagaaa aaacgttgtc gccgctggtg gtgcacaaca atctgcaaat
     6961 cgatctcaat gagccggacc tctttctcga cagcgactct ctgagccagc ttcccaaaga
     7021 tctcctcacc attccgtttc tccacgatgt tctgagtgaa gatttcgttt tctattatca
     7081 gaatcatgcc gatcgtctgg gcattgaagg cagcattcgt cgcattgtct atgaacacga
     7141 tctcacgctg aaagataaac tcttttcgtc actcttagat ca
//
LOCUS       DAJTNA010000189         7170 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTNA010000189 DAJTNA010000000
VERSION     DAJTNA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.191"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            384..1358
                     /gene="hemB"
                     /locus_tag="OO161_003475"
     CDS             384..1358
                     /gene="hemB"
                     /locus_tag="OO161_003475"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5464415.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1464..2305)
                     /gene="tauD"
                     /locus_tag="OO161_003476"
                     /pseudo
     CDS             complement(1464..2305)
                     /gene="tauD"
                     /locus_tag="OO161_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2302..3129)
                     /gene="tauC"
                     /locus_tag="OO161_003477"
     CDS             complement(2302..3129)
                     /gene="tauC"
                     /locus_tag="OO161_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5464416.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3126..3893)
                     /gene="tauB"
                     /locus_tag="OO161_003478"
     CDS             complement(3126..3893)
                     /gene="tauB"
                     /locus_tag="OO161_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5464417.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHDSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3906..4868)
                     /gene="tauA"
                     /locus_tag="OO161_003479"
     CDS             complement(3906..4868)
                     /gene="tauA"
                     /locus_tag="OO161_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5464418.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQAGNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKVIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            5484..6155
                     /locus_tag="OO161_003480"
     CDS             5484..6155
                     /locus_tag="OO161_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCR5464419.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     IPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            6165..7170
                     /locus_tag="OO161_003481"
                     /pseudo
     CDS             6165..7170
                     /locus_tag="OO161_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         1793 a         1895 c         1715 g         1767 t
ORIGIN      
        1 tatataattt atggagaaaa acacgcatca aattgataaa aggacgatat ttcaacatgt
       61 tgcagataat attatttttg atacatacgc gttataaata tcaaaaacac aaaccagata
      121 aatatcttat caatatacaa caaagtttga attattcaat ttatagcagc acaaccaaac
      181 gccaaaaaaa ataataacca agagctataa ataaagatta aaagtatttt cagtgaatat
      241 gaacttcttg ctatttctat tacaaagatg tcctggtctc tctttcattc tcttcttgtc
      301 aacatcacga caactttcgt aaaacactcc tgccccgctt caggtatact gtgcacctta
      361 attccacaaa catcaggcag accatgacag acttaatcca acgccctcgt cgcctgcgca
      421 aatctcctgc gctgcgcgct atgtttgaag agacaacact tagccttaac gacctggtgt
      481 tgccgatctt tgttgaagaa gaaattgacg actacaaagc cgttgaagcc atgccaggcg
      541 tgatgcgcat tccagagaaa catctggcac gcgaaattga acgcatcgcc aacgccggta
      601 ttcgttccgt gatgactttc ggcatctctc accataccga tgaaaccggc agcgacgcct
      661 ggcgggaaga tggactggtg gcgcgtatgt cgcgcatctg caagcagact gtgccagaaa
      721 tgatcgtcat gtcagacact tgtttctgtg aatacacttc tcacggtcac tgcggtgtgc
      781 tgtgcgagca tggcgtcgac aacgacgcga ctctggaaaa tttaggcaag caagccgtgg
      841 ttgcagctgc tgcaggcgca gacttcatcg ccccttccgc cgcgatggac ggccaggtac
      901 aggcgattcg tcaggcgctg gacgctgcag gctttaaaga tacggcgatt atgtcgtatt
      961 cgaccaagtt cgcctcttcc ttttatggcc cgttccgtga agctgccgga agcgcattaa
     1021 aaggcgaccg caaaagctat cagatgaacc caatgaaccg tcgcgaggcg attcgtgagt
     1081 cactgctgga tgaagcccag ggcgcagact gcctgatggt taaacctgct ggagcgtacc
     1141 tcgacatcgt gcgtgagctg cgtgaacgta ctgaattgcc gattggcgcg tatcaggtga
     1201 gcggtgagta cgcgatgatt aagttcgccg cgctggcggg tgctatagat gaagagaaag
     1261 tcgtgctcga aagcttaggt tcaattaagc gtgcgggtgc ggatctgatt ttcagctact
     1321 ttgcgctgga tttggctgag aagaagattc tgcgttaatt tttttcctct cagaaagggg
     1381 gagttaacct gttgaaagta cggtgaacgc cccctcaccc taaccctctc cccaaggggg
     1441 cgagggaacc gtccgcactc gtatcacccc gcccgataaa acggtttatc cccaagaatc
     1501 gtcacccgat gcataatccg ccgctgtggc aggtaatcgg cattggcata gtgctgagtc
     1561 acgcggttat cccaaatcgc aatatcattc ggttgccagc gccaacgcac ctgaaactcc
     1621 ggtttagtga tatgggcgaa taaaaagcct aacaaggctt cgctctcttt ctcgctgaca
     1681 tcgacaattc gcgtagtaaa gccttcattc acaaacagcg cctgtttacc gctcaccgga
     1741 tgcgttcgca ccaccggatg tagcaacgga ggatttttcg cgaccgcctc gcgccagcgt
     1801 tgatgttcct cctcggtttt gcggtatttg tattccggga acgatttacg gaaatcatgc
     1861 tccgcacgca gcccgctcag cagctggcgg aagggaacag agagcgcctc ataggccgca
     1921 ataccgctgg tccagagcgt atcaccgccg gtcgaaggta attctttagc tgccagaatc
     1981 gcccctgcgg gtggcgtttc aataaatgtc acatcggtat gccagttgtc gttatctggc
     2041 ggattatcgt tatgggtatc cagcacgatg atctcgtcaa ccccttcggc atgagggtaa
     2101 acagggtgaa tatgcagttc gccaaaacgc tgggccagcg cgcgttgctg ctgcggcgta
     2161 atggcttgat cgcgcagaaa caccacctga tggcgcagca ccgcatggta aagcgattat
     2221 cgcttaacgg gcgcgtcagg tcggcacccg aaatttgtgc gccgatatac ggccccagcg
     2281 gggtaatgct cagacgttca ctcattgtac ttctccatgc cagggcgtca gacggcgctg
     2341 taacgcgcgc aaacccagtt ctaaaagaaa ggcgataatc gcaatcaccg cgattcccgc
     2401 cagcaccacg tcagtcgcga gaaattcacc cgctgactga accataaatc ctaaaccgcg
     2461 cgtcgcggca atcagctccg ccgctaccag cgtagaccag cccacaccca gaccaatgcg
     2521 taatccggtg aggatttccg gcagcgcgcc gggcaaaatg acaaaccaca gcacctgcgc
     2581 acggctggca cccagcgact gggcggcacg aatgcgaacc tgctgcgcgc ttttcacccc
     2641 cgccagcgcc gacatcgcca ccggtgcaaa aatcgctaaa tagatcagta agatcttcga
     2701 ggtttcacca ataccaaacc agatcaccat cagcggcaaa taagccagcg gcgacaccgg
     2761 acgataaagt tcgattaccg gatccagaat gccgcgcacc gtcgggctta gccccatggc
     2821 gatcccgacc ggaataccga tcaccaccgc cgcgagtagc gccagcacaa tgcgcgtcag
     2881 gctggctgcc agatgctgcc acagcgtggc gtccataaat ccctgcggtc cggcaatggt
     2941 gagcagtttt gccagtactt gttgcggcgg cggcaaaaac agcgggctaa tcagttgcag
     3001 cgccgccacc gtccaccata cagtgagtaa aaccgctaac gtgccaatgc ttaaggtcac
     3061 ctgacgcgag agcggccagc gccatttcag ccgccgcgaa tgcagttttt cattaatgag
     3121 cacactcatg agaacgcctc ccgttgctca aatacgcggc ttaaaacata ttcgcgcatg
     3181 gcgatgaatt gtggatcgga cttgatgctg cggctcgact ctcccgcaac aaagcggcga
     3241 gcaaagttga gcggcagccg ctccagcaca cggccagggc cggatgaaag cagaaccagt
     3301 tcagtcgcca taaacaccgc ttcttctata tcgtgggtaa tcaacagcac ctgcttgccc
     3361 gtctcctgcc agagtttcag cagcagagtt tgcatctggt cgcgggtgaa ggcgtccagc
     3421 gcaccaaacg gttcgtcgag taataacagc tggggattcg ccgccagcgc gcgggcaatc
     3481 cccacccgct gacgttgacc accggaaagc tgccagatat agcgtttttc tgcgccttcc
     3541 agccccactt ttttcagcac ctggtgcgcg atttccagtc gctgcatttt ctctatacct
     3601 gccaattgca ggccgaacgc cacgttgtcc tgtacattgc gccacggtag cagcccttca
     3661 ttctgaaaaa ccaccccgcg ctctgctccc ggtccctcaa tacgcttacc cgccagttga
     3721 atgctgtcat gctgataagg cacaaaaccg gcaatcagat tcagcaacgt ggttttaccg
     3781 cagccggacg gccccagcac caccagtagc tcgccgcttt ccagcgtcag gttgatatct
     3841 tccagtgccg gtttgccgcc ataatcggca tagagatgag agatttgcag catctgcgcc
     3901 tccttttatt gcacgaagcg cgaggtaacg tactggctgt aatcattcgc tacagccggg
     3961 accttgccct gctctttcaa aaactgcgcg gtgtcgatga ttactttgtt caccggtccg
     4021 gtcagttctg ccgtttgttg ctgcggcgtc agataggtat tccccttaac cagccccgga
     4081 acgtcacctt ccggcacgcc gcttaaacgc gccagtttgc tgatgttttc cggctgtttc
     4141 agccacgcgt ctgggttagc aatgtacggt tgctgagcat cgatggcgct tttagcgaac
     4201 gctttcacga cctcaggatg tttctcggca aaatctttgc gcaccaccca gacgtccagc
     4261 gttggcgccc cccactgccc gacctgttca gaatcggtca acaccttgcc gtctttttcc
     4321 agggcgttaa ccgccggtgc ccagacataa gcaccatcaa tatcaccccg ctgccatgcg
     4381 gcgataatcg cgggcggctg caggttcaca atctccactt gccctggttt aatcccccag
     4441 tgtttcagcg ccgccagcag gctgtagtgg gtggtggaga taaacggtac ggcaatgcgt
     4501 ttgccaatca aatcttcggg tttgctgata gttttcttta ccaccagcgc ttcggagtta
     4561 cccagttttg acgccagcaa gaagacttca atcggcacct gttggctggc tgcaaccgct
     4621 aatgggctgg aaccgaggtt gccgatttgc acgtcgccag aagccagcgc ccgcacgatg
     4681 ctggctccgc tgtcaaactt acgccagtcc acgctcgccc cgctctcttt agcaaaggtg
     4741 ttgccggcct gagccacttt cgccggttcg gctgaggttt gatacgccac ggtgacgttc
     4801 accgcctgtg cctgaaaagc gatgaatgcc agtgcggcaa gaagtgtgtt acgcgatgaa
     4861 attgccatga ttgtctgctc cccagtcttg ttatgggagc agtattcagg aataaaaaca
     4921 ttcattaaaa gaattagtcg ttatcgcaca gatgatttta ttcttagcaa aaaaacggca
     4981 aaggaaagat taaatatgca attactccgt tctaacaatt gggaaaatta ctacacttca
     5041 ttttagtgat gcataaacaa ccacattacg agatttatta cttcattgtt tctatcaata
     5101 tcaatacata taataaaact attatattcg cattttatat atcactgtat cattctcaat
     5161 cactcgcata ttacattcac ataatgtata taaacaatac ttgttcacag aacatatttt
     5221 ttaaatgcaa ttaattgaca aactaaatca catctaaata caatgtataa aacaaaataa
     5281 ctatacatac caaacataca aacacttaat atatgaattg cataaaaaca ttacccagtc
     5341 gaacctccgg gtctgaaagc ccttgctttt cagttattag caacatttaa catttataaa
     5401 ttaacatctg gctgcaccag agattacgtc tctgagtgca tcgttgtgct tgtagtataa
     5461 taaataaaat ggagtgtcat taagtggata aggttttaga ttcagccctc ctttcttcgg
     5521 caaataaaag aaagggtatc cttgctattg gcgcacatcc tgacgatata gaattaggct
     5581 gtggcgcatc gcttgctcgt cttgcgcaaa aaggaattta tatcgcagca gtggtgatga
     5641 ctaccggcaa ctctggtaca gatggaataa tcgatcgcca tgaagaatcg cgcaacgccc
     5701 taaagatatt agggtgccac caaactattc atcttaattt tgctgacacc cgcgctcatt
     5761 tacagctcaa tgatatgatt tccgccctgg aagacatcat taaaaatcaa attccttctg
     5821 atgttgaaat catgcgggta tataccatgc atgatgccga ccgccatcag gatcatctcg
     5881 ctgtttatca agcttcaatg gttgcctgcc gcactattcc acaaattctc ggctacgaaa
     5941 ccccaagtac ctggctttca tttattcctc aggtttttga atccgttaaa gaagaatatt
     6001 tcacagtcaa acttgctgca ttaaaaaaac ataaaagcca ggaacgacgt gattatatgc
     6061 gtcatgatcg cctgcgtgca gttgcacaat ttcgcgggca acaggtcaat agcgatctgg
     6121 gtgaaggctt tgtaattcat aaaatgattc tttgagcaaa ttgcatgaaa acctggatat
     6181 ttatctgtat gtccatagca atgttgctat ggtttttaag tacgctaaga cgtaaaccca
     6241 gtcaaaagaa aggctgtatt gacgccatta tacctgcgta taacgaaggc ccgtgtctgg
     6301 cgcagtcact ggataatcta ctgcgtaacc cttatttttg ccgggtaatt tgcgttaacg
     6361 acggctccac ggacaatacc gaagcggtca tggcggaagt caaacgcaaa tggggcgacc
     6421 gctttgttgc cgtcacgcaa aaaaaatacc ggtaaaggtg gtgcgctgat gaatggcctc
     6481 aattatgcca cctgcgacca ggttttttta agtgatgccg acacctatgt tccgcccgat
     6541 caagacggaa tgggctatat gctggcagaa attgagcgcg gggctgttac cgcacatccg
     6601 cgcgaccgta aagttgccga tgattgttat gaagcgcacg ctacagcagc tcctgggcgg
     6661 cgcaccgttt attatcagcg gtgcctgcgg aatgttccgt actgatgtat tgcgtaagtt
     6721 cggtttctcg gatcgtacta aagtcgaaga ccttgatctc acctggacat tagtggcaaa
     6781 cggctaccgt attcggcagg cgaatcgctg catcgtatac ccacaggaat gcaacagtcc
     6841 gcgtgaggag tggcgtcgct ggcggcgttg gattgtgggc tacgcggtct gtatgcgcct
     6901 gcataaaagg cttttattta gccgcttcgg tatcttcagt atatttccta tgctgttggt
     6961 tgtgatttat ggcgttggga tttatctcac tacctggttt aatgaattca tcaccaccgg
     7021 gccgcatggt gtggtgttgg caatgtttcc gcttatctgg gtcggcgtag tttgtgttat
     7081 tggtgctttt agcgcctggt ttcatcgttg ctggttgttg gtgcctttag cgccgctttc
     7141 cgttgtgtat gtattattag cttatgccat
//
LOCUS       DAJTNA010000190         7164 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTNA010000190 DAJTNA010000000
VERSION     DAJTNA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.192"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..129
                     /locus_tag="OO161_003482"
     CDS             <1..129
                     /locus_tag="OO161_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCR5464420.1"
                     /translation="TAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(183..1298)
                     /gene="rnd"
                     /locus_tag="OO161_003483"
     CDS             complement(183..1298)
                     /gene="rnd"
                     /locus_tag="OO161_003483"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5464421.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPAITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(1380..3065)
                     /gene="fadD"
                     /locus_tag="OO161_003484"
     CDS             complement(1380..3065)
                     /gene="fadD"
                     /locus_tag="OO161_003484"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5464422.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDGNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(3270..3851)
                     /gene="yeaY"
                     /locus_tag="OO161_003485"
     CDS             complement(3270..3851)
                     /gene="yeaY"
                     /locus_tag="OO161_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5464423.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPAQPI
                     EPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(3891..4586)
                     /gene="tsaB"
                     /locus_tag="OO161_003486"
     CDS             complement(3891..4586)
                     /gene="tsaB"
                     /locus_tag="OO161_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5464424.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(4644..6554)
                     /locus_tag="OO161_003487"
     CDS             complement(4644..6554)
                     /locus_tag="OO161_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5464425.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGELGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            6686..7030
                     /locus_tag="OO161_003488"
     CDS             6686..7030
                     /locus_tag="OO161_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5464426.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLV
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         1702 a         1978 c         1780 g         1704 t
ORIGIN      
        1 acagcaatac tggaaggtga agctgaccat cgggatcagt attttagcga tgaagagcgt
       61 gccagccagc aaagtatgtt gatctgttgt tcgcgtgcga agggtaaacg cctggtgttg
      121 gatttgtagt ttgccaaagc cggatgtggc gctgagcgcg cccagtccgg cttcggaaga
      181 ttttactgcg gatattcctg caataaattg tgtaatgctt ccgccatcag ctcaccacgc
      241 cagccggaaa tcagctccgg caaattgttc tgcggtttca gtttccagtg ccagttcagc
      301 agttggttga tttgccgacg cgatgccagc aattcggcgc tgatcttatg cgtttcgctc
      361 acgtcagtaa tcagcgactt aatcgcttta aacgctttac gataaccagg catgtccatc
      421 aggttaagca tcggctgcgg taaggcctct tccggcaatg tctgcgcttt ttccaccagc
      481 gtcagcagcg ttttaccgtg aaagcggatt tcgctaccgg ataaacccag gctgtccagt
      541 tcgcctaaac ttcccggcat ataacgcgcg accgaccaca aatgctcttc acgcacgaca
      601 aagttcaccg ccagatcacg ctctcgcgcc ttgcgcagtc gccagtcggc taacagttgc
      661 agacaggcca gttggcgtgt gcgtaactgc caggcattgg tgatatcacg ccaggcatct
      721 tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc ggcattcatc cagcgccgca
      781 ggtagccagc cggaggcctc cgtttctacc ataagcttgg cggtgatcgg taacagatac
      841 cagacatccg ccgctgcgta ttcacactga cgttcggtca gcggtctggc cagccagtcg
      901 gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact cttccaccat ggaagcgaaa
      961 ccccatgaca tcgggcgtcc gcagaaggca gaaaggattt gcgtgtcaat caagggttgt
     1021 ggtaattcgc caaagacatt gaggaacact tccagatctt cactgcctgc atggagaaat
     1081 tttgtgatgg ccggatcgcg caggatcgct ttcagcggtg accagtcggt gatcccgaga
     1141 ggatcaatta gcgccagatg ctcgccatcg aaaagttgaa tcaaccccag ctgcgggtaa
     1201 taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg ccggaaaggc acggacggct
     1261 tcacacaaag aagccagcgc atcgtccgtg gtaatcattt ggtaactcaa attgttttct
     1321 ctttagtggg cgtcaaaaaa aacgccggat taaccggcgt ctgacgactg acttaacgct
     1381 caggctttat tgtccacttt gccgcgcgct tcgtcacgta attctcgtcg caaaattttt
     1441 ccgacgttag atttcggtaa ctcatcacga aactccacca gcttaggtac tttgtatccc
     1501 gtgagctgac ggcggcaaaa agtcaccagt gactcttcgg taagcgatgg atcttttttc
     1561 actacgaaga ttttcaccgc ttcaccactg gagccggaag gtacgccaac agccgcgact
     1621 tcctgtacgc caggatgctg catgacgaca tcttcaatct cgttgggata gacgttaaaa
     1681 ccggaaacca gaatcatgtc ttttttacga tcgacaatgc gcaggaatcc ttcttcatcc
     1741 attaccgcga tgtcgccggt gtgtaaccag ccatttttga tgatttcatc ggtagcatcg
     1801 ggacgctgcc agtaacccag catcacctgc ggtcctttga cacaaagctc acccggttga
     1861 cctggtggta cttcattacc atcatcatcc accagtttgg cttccgtcga cggcaccggc
     1921 aaaccgatgc taccactatg ataatcaata tcatatgggt taacgctgac cagcggcgca
     1981 cactcggtaa ggccatagcc ttccagcaga tactgtccgg tcagtttcac ccaacgctct
     2041 gccaccactt gctgcactgg catcccaccg cctgcggaaa gatgcagact ggagaaatcc
     2101 agctgctgga actctttatt gttcagcaac gcattgaaca aggtgttaac gccagtgata
     2161 gcggtaaacg gatatttcgc taactctttt accaaccctg gaatatcacg cgggttagtg
     2221 ataagcaggt tctgcccacc cagttcgata aacagcaggc agttaatggt cagggcaaaa
     2281 atgtgataca gcggcagtgc cgttactacc agttctttac ctggatgcag caacggccca
     2341 taggtggcgt tgacctgttc caggttcgcc agcatattgc ggtgagtcag catcgcgcct
     2401 ttcgccacac ctgtggtgcc gccggtgtat tgcaggaagg ctaaatcttc cggcaccagt
     2461 tcgggtttga cgtactgcat ccggtagccg ttatgcagtg cgctacgaaa tgaaatggca
     2521 tctggcagat ggtatttcgg caccaaacgc ttgatgtatt taacaacgaa attgactacc
     2581 gtgccttttg ccgtagatag ctgatcgccc atacgggtca gaattacgtg ctgaacggcg
     2641 gttttatcaa ccactttttc cagtgtgtga gcaaagttag acacgataac aatcgccgat
     2701 gcgccgctat cgttaagctg atgctcaagc tcacgcgggg tatacaacgg gttaacgttt
     2761 acgacgatca tcccggcacg caaaatgcca aacagcgcca ccggatattg caataaatta
     2821 ggcatcatca acgcaacgcg atcgcctttc ttcagcccca acccttgttg caaataagcg
     2881 gcaaacgcgc gactgcgttc ttccagcttg cggaaggtca tcacctcccc catattcaca
     2941 aacgcaggct gatcggcgta gcgcgcgacc gactgctcaa acatatctac cagagattga
     3001 taacggtcag ggttgatctc cgtcggaacg tccgcgggat aacggttaag ccaaaccttc
     3061 ttcaattctt cacctctaaa atgcgtgttc gtcgtcatcg caaccccaaa tgatatacat
     3121 gccattaaca taatattaac tcatcatacc agcttgataa ttacccaacg aaaaggttgc
     3181 gaagcgcgtc actatttatt tttatcttta ccgtaagaat gcagaaacag cggaccagcc
     3241 gctgtttctt tttctttaaa aacaagcaat tattcagtta caactgtttg tactctcgcg
     3301 ggaccgggat tataccagcc ccatccgccg tagccatagg gccagccacg accgccataa
     3361 aaccatggct caatcggctg agccggcata atcacctgct gggttaaatg ccaacgtttg
     3421 taacccgtta cttgcatcac cataaattta tagggcgtat tgccgatttt gccgtcaacc
     3481 gcaccagtga ttggcccgac taccgtaacc agttgtccac ggaaatccac cgggtccaga
     3541 aaaccgttca catcggcata aatgcgaccg cgagaaggtt ctcccagcgt cggtctggct
     3601 ccgctgtcca gcggtacggt agcaatttcc aggcgggttt tcccttgctg gttttgtacc
     3661 gcaaccactt tgccaccaaa gcgtgcctcc tgaccaacgt acagctgcgg cgcactcatc
     3721 acccgaacta aatcttgttg cggcgtggga ctgctgcctt taatggcgtc cggcacagtg
     3781 acacaaccgc tcagcattag cgcaaacgta cctgccagta tgcctttgat aacattcttt
     3841 tgaaccgcca tgatgcgact cctttttttc tcagggcata ctcttaagat tcattctttg
     3901 cccggaagtt tcttccatgc gacgttgtta cgtaaataaa ccggttcggc atgttccacc
     3961 gccaccgttt taccctcggc aaatatctga cacgcaatcg gcagcatatc ttccgtcgct
     4021 ggcagtaaaa cttctccgtc gcgcaaaacc agcccgctct ctttaccgag atccggccag
     4081 gcttgccagc ccgtccctac cgttacccat tcgccggaaa gctgttgcat tcgttcatga
     4141 acgagttcgg gtttgagtac ggcttcggtt tcttcaccgc gccagatacc gttttcatca
     4201 cgctgatatt cggcccagta aacttcgccc atccgcgcat caatggcagc cagcacgcgg
     4261 gttgcgccgt ttttgcgcca cgccccttgc gccattgtca ttagcgtgga gacgccaatc
     4321 atcggcagct ctgcgccaag tgccagccct tgcgcaatgc caataccaat gcgcacgccg
     4381 gtaaagctac cggggccgcg cccgtaagcc agagcgttaa tatcagtcag ggaagttccg
     4441 ctggtggtca ggatatcctg caccatcggt aagattcgtt gagtatgttc acgagggcaa
     4501 agctcaaaat gagcgttgac agtaccgtcg ttccacaggg cgacagagca ggcctctgtc
     4561 gcggtatcga tagccagaat tcgcatgggt cttcgtgctt agatcaataa aaaggcgcgc
     4621 atcataccat actccgtaac aaattacctg gaggatggta tcgcaaggaa acgaaccgca
     4681 cgggcaatgt cacgggtgcg cggcgcgggc ggcagactgg cgagaaacgt cgcgccgtaa
     4741 gggcgcatca ccagccgatt gtcacaaatc accaaaaccc cgcgatcgtc ggcgtcgcga
     4801 atcagtcgcc ctaccccctg cttgagagta atcaccgcat ccggcagttg tacttcatcg
     4861 aacgggtcgc caccgcgcaa acggcaatct tccatgcgcg cttttaacag cgggtcatcc
     4921 ggtgaggtaa acggcaactt gtcgataatc accaatgaca atgtatcgcc acgcacgtcc
     4981 accccttccc agaaactact ggttgccaca agaagcgcgt taccggcgct gacaaattgc
     5041 tgcaacagtt gccctttgct ggtttccccc tgcaacaaaa cgggaagcgt catggtagcg
     5101 cggaactgct cggccagatc acgcatcatg gcgtgcgagg tacaaagcat aaaacaacga
     5161 ccgttgttag cttcgatgat cggtcgcagc attgccgcta actggcgagc agaacctggc
     5221 tggttggttt gcggcagatt gcgcggcaca cagagtaacg cctggcggct gtaatcaaac
     5281 gggcttggca atagcaacga ctccgcctgt tctatcccaa gccgcgaggt gaaatgatgc
     5341 agatcgtcgt tcaccgacag cgttgctgag gtgaagatcc agctaccagg cttttgcgcc
     5401 attaactctt tgaatttatc cgccacgctg agcggcgtga gagccagagt aaaatggcgc
     5461 gaagtgcatt cgtaccagta gctgtagccc ggctgattga tctcttttag ccgcttcagc
     5521 cgtgtgcgat acaacgtagc gcgctcaaat gccgcatcca gcaaggcgga acgccccagt
     5581 gacagtttcg ccacgtcata acaaagttcc agggtgtcat cgagcagtaa aaatgcccgc
     5641 tgaatttgcg gattagctaa cagctcacgc aggttaccgc gataacctag ctcaccgagt
     5701 tgcagacgaa aatcctgcgc actctgggca agacgatcag cgcacttttg taactgctgg
     5761 gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt ttgccaggtc gagcagttgt
     5821 cgactggaga gtgactgacc aaaatactgg ctggcaatgt ccggtagctg gtgggcttcg
     5881 tcgaagatca tgacgtccgc ttccgggatc agttcgccaa atccactctc tttaaccacc
     5941 atatccgcca gaaagagatg atggtttacc accaccacat cggcgtccat cgctttttta
     6001 cgtgctttga ccacaaagca atctttatac atcgggcagt cgctgccaag acagttgtcg
     6061 ttggtgctgg taaccagcgg ccacgcctgt gaatcttccg ccacgctgac gcaggtgctg
     6121 atatcaccat cgactgtttg attagaccag gagcgcagca ggatcacatc gcttaagatt
     6181 tgtaccggca gatcgccccc cgccagcgcc tgctgttcga gacgttcgag gcagaggtag
     6241 tttgagcgcc ctttcagcag cgccacgttg cccgtatatt tcaatgcctt tgagactgtt
     6301 ggcaaatcgc ggctgtagag ctgatcctgc aacgcttttg agccggtcga gataatgact
     6361 ttctttttcg cccgcagcgc aggagccagg taagcgtagg ttttgcccgt accggttcct
     6421 gcttccacca ccagcggctg gcctttttct atcgcctggg tgacggctac cgccatctgt
     6481 cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg ccagctgacc gtctggtgca
     6541 aaatcgtccg tcacactacc ccctgttgat ttgaacaggg attatgtcag gatgagggcg
     6601 ctttcgccag ttgaagtggt gacggcaacc tcacattgtg gcagtctttg cagcacgaaa
     6661 atggaagtat aatgaggaca aaatgatgac tatcgttcgt atcgatgctg aagcccgctg
     6721 gtctgatgta gtaatccaca acaacacgct ctactacact ggtgtaccgg aaaacctcga
     6781 cgccgatgct tttgagcaaa ccgccaacac gctggtgcag attgacgccg tgctggaaaa
     6841 acagggcagc aataaatcga gcattctgga tgccaccatt ttcctggctg ataaaaacga
     6901 cttcgcggcg atgaataaag cgtgggatgc ctgggttgtg gcgggtcatg cgccggtgcg
     6961 ctgcacggtg caagcggggc tgatgaaccc gaagtataaa gttgaaatta agattgtggc
     7021 tgcggtgtaa gctttatcga agcaaaataa gtcagacgat aatttatcga taatactggt
     7081 cggttttaca taaatcgacc agagattaag atattactgt tgatgctcct gctaattcat
     7141 tatttatttt tataatttca attt
//
LOCUS       DAJTNA010000191         7055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTNA010000191 DAJTNA010000000
VERSION     DAJTNA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.193"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..102)
                     /locus_tag="OO161_003489"
     CDS             complement(<1..102)
                     /locus_tag="OO161_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5464427.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEF"
     gene            complement(115..1896)
                     /gene="gcl"
                     /locus_tag="OO161_003490"
     CDS             complement(115..1896)
                     /gene="gcl"
                     /locus_tag="OO161_003490"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5464428.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTTGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGGIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAVDAPTETCFMHYE"
     gene            complement(1986..2801)
                     /gene="allR"
                     /locus_tag="OO161_003491"
     CDS             complement(1986..2801)
                     /gene="allR"
                     /locus_tag="OO161_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5464429.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(2879..3361)
                     /gene="allA"
                     /locus_tag="OO161_003492"
     CDS             complement(2879..3361)
                     /gene="allA"
                     /locus_tag="OO161_003492"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5464430.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3591..4493
                     /gene="allS"
                     /locus_tag="OO161_003493"
     CDS             3591..4493
                     /gene="allS"
                     /locus_tag="OO161_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5464431.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNIVINNLLYNPQAVAQ
                     LLAWLNERYPFTQFHISRQIYMGVWDSILYEGFSLAIGVTGTEALANTFSLDPLGSVQ
                     WRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDME
                     TKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVED
                     IVTLFTQRRPEISGFLEIFGNPRS"
     gene            4562..5656
                     /gene="mnmH"
                     /locus_tag="OO161_003494"
     CDS             4562..5656
                     /gene="mnmH"
                     /locus_tag="OO161_003494"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5464432.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            5772..6137
                     /locus_tag="OO161_003495"
                     /pseudo
     CDS             5772..6137
                     /locus_tag="OO161_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(6699..>7055)
                     /locus_tag="OO161_003496"
     CDS             complement(6699..>7055)
                     /locus_tag="OO161_003496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464433.1"
                     /translation="LYKDESLVSKHYINYMAIPENDGVFTWLPDFFSHVAVDISIYTN
                     VEDDYFFSYFSLTNDEEGRFKKTLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPGQGD
                     GVNLFLLVKLMLRIIL"
BASE COUNT         1806 a         1816 c         1688 g         1745 t
ORIGIN      
        1 aaattcaacg ccgcgaaaac cacactgcgc agctttctca aaacgggcga gaaaatcata
       61 ttcaccaaat aacatcgata aattagcaga gaaacgtaac attatttatc tcccttattc
      121 atagtgcatg aagcaggttt cagtcggtgc gtccactgcg ttatcggcga tatcttcaaa
      181 ttccatgacg ttatccagtt cgctgcccat cgaaatattg gtcacacgct cgagaataac
      241 ttccacgact accggtaccc gatattgcgc cattaaggct ttcgcctgtt caaaggctgg
      301 cgcaatatct tccggtttga agacgcgaat cgctttacaa cctaaacctt ccgctacttt
      361 tacgtggtcg acgccgtaac cgttcacttc gctggagttg atattctcga aagcgagttg
      421 cacgcagtag tccatgtcaa aggcgcgttg tgactgacga atcaggccga gataggcgtt
      481 attcaccagc acatggatgt acggaatgtt gaactgcgcg ccaacggcta actcttcaat
      541 caggaactgg aagtcaaagt cgccagaaat cgccaccaca ttgcgtttcg gatcagcggc
      601 acaaacccct agcgcagccg gaatcgtcca gcctaacgga ccagcctgac cacagttgat
      661 ccagtggcgg tctttaaaga catgcagcat ttgtgccgca gcgatttgtg acagaccgat
      721 ggtggtgaca taacaaacat cgcgaccaaa ggctttgttc atctcttcat acacgcgctg
      781 cggtttcacc ggcacgttgt cgaagtgggt tttgcgcagc aaagtgcgtt tgcgctgctg
      841 gcagtcggcg acccattctt tacgacacgg cagacgaccc gctttttgca tctcctgcgc
      901 cacttcaacc agcagtgtca gcgccgcttt agcatcagag acaatgccga gatccggaca
      961 cagcacgcga ccaatttgcg tcggctcaat atcgatatga acgattttgc gcccttcggt
     1021 gtatttctct accgaaccgg tatgacggtt agcaaaacgg ttaccgatac caaacaccat
     1081 gtcggacgcc agcaacgttg cgttaccgta acgatgcgcg gtttgcagac cgaccatccc
     1141 ggccatcagt tcatgatcgt ccgggatacc gccccagccc atcagcgttg ggatcaccgg
     1201 aacgctggtc agttcagcaa actgttgtaa cagtgcagct gcgtcagcat tgattacccc
     1261 gcccccggca acaatcaccg gacgttcggc ctggattaac atttctacag ctttttcgat
     1321 ctgcatacgg ctggcagcag gtttgtagac cggcagcggt tcgtacatgt caggatcaaa
     1381 ctcgatttcc gcaacctgaa cgtcgaacgg taaatccacc agtaccggac ccggacgacc
     1441 agaacgcatc aggtgaaatg cctgttgcag cacgcgaggc accagcgccg cttcacgaac
     1501 tgtaaccgcc attttgctga ccggtttagc aattgcttca atatctacgg cctgaaaatc
     1561 ttctttatgc agacgggcgc gcggtgcctg gccggtaatg cacagaatag gaatggaatc
     1621 agcagaagcg gaatagagcg cggtgatcat gtccgtgccc gcaggaccgg tagtccccag
     1681 acatacgccg atattccctg ccgttgcgcg ggtataacct tccgccatgt gcgaagcacc
     1741 ttccacatga cgcgccagaa tgtgacgaat accgccgtgc ttacgcatcg ctgagtagaa
     1801 cggattgatt gcagctcccg gaacaccgaa ggcggtggtg ataccttctt tctccagcac
     1861 atacattgcc gcgtcaacgg ctctcatttt tgccatttta ttcctatctc tattcattgg
     1921 aaaatttttc caactttcaa attttgtcaa aaaaacccgc tccgcagagc gggatgcgac
     1981 agacattatg gatgtgcttt cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg
     2041 accagctcac cctgactgac aaaacgatct tctgtcagtc ttgatgaagg cccggagatg
     2101 gagatagcgg caacaacgct accgacatca tcgtaaattg ctgaagctat gcaattcaga
     2161 cctacaacat gctcttcttt atctacggta tagcccagtt cacgcgcttg ttccaggtcc
     2221 ttcagcaagg tgggcatatc cacaagcgta gttggcgtaa actgctgcaa accggtttgc
     2281 agaatgatgc tcatcaactc ctcttccgcc agcggataaa gcagcgcttt gcccgcaccg
     2341 gaagcatgca gcggcagacg actgcccagt ggcgcacaca tcctgaccat tgatttacac
     2401 tctaactgac caattaatac tgcttcattg ccgttacgga tcgcgacatt gaccgtttcg
     2461 ccggaaagta acatcaggcg gcgcataaac ggcccggcga cggagaggac atcgcggtta
     2521 tggatgtacg ccgcaccgac gttaaagaca cctaatccta tatgccacca gcctaattga
     2581 ctgtcctgat agacaaaatc cgctgcttgt aaaaccttca gcaagcgaaa ggtcgtggag
     2641 agcggcaaat ccagattgag agaaatatcg ctaaccgacg aacttccccc acttttttcc
     2701 agatattgca gaatcgcaat tccccgctct aacgcctgtg cgcccttctg tgccacaggc
     2761 tccgcctgtc ctggcctgcc gcgccgtcta acttccgtca tacctacgtt aactccgacc
     2821 tacaagtgag cataaacaat gaacaattag tttatcttat gcaagtcggt tgcaggcgtc
     2881 acgcaaaaca gagttcctgt tcaggaatac tttcaacatc acagttgtca ctgccgccgc
     2941 gatcgatggt cagaaaatcg gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt
     3001 tacgatggta attcactccc tgttcgccgt tggtgataaa cgcccgcagc gttgacaggt
     3061 ctggtttgtc gtcgcctaac gccacgacca ccacaaacac ttcacctttc atcgggataa
     3121 aggcctgagt gcccagcgga tgacgttcga gttcgtgaat ggtcagcggc agattcgccg
     3181 gttgcgcgcg gttaatgcta ataagcgtac ggtcttgctc aagaatctca accagcgcca
     3241 aatcgtggta acgctccacc aggccattgt taatatggaa aaaatcccgt tgctgagttt
     3301 cgattacgtc tccataagca ctaaaggctt cctgacttaa cggtaatacc tgaagtttca
     3361 tctttttgtc cttgtaacat aattccgttc aaccaccgcc gaccggtcag cttggtttca
     3421 ataacaggca atctaaaacc ttaagagaac aacatcaata caaaagttga aatatctttc
     3481 caaaactaga tttagtccac aaattgaaca gggttatcat gtaaaaatac tgataaatca
     3541 gaggataaga aaatcacgca acttgcggat ttaaaagggg atacctgaag atgttcgatc
     3601 cagaaacctt gcggactttc attgcggttg ctgaaacagg aagtttttca aaagcggcag
     3661 aacgattatg taaaaccacg gcgacgatca gttatcgcat taaacttctg gaagagaata
     3721 ccggagtagc gctgtttttc cgtacgactc gtagcgtaac gttgacagcg gctggcgagc
     3781 atctactttc ccaggccaga gactggctga gctggctgga aagtatgccc agcgagctgc
     3841 aacaggtgaa tattgtcatc aacaacctgc tctataaccc ccaggccgtc gcccagttgc
     3901 tggcgtggct gaatgaacgt taccccttta cccagtttca catctcccga caaatctata
     3961 tgggcgtctg ggactcgata ttgtacgaag gtttttcgct ggctatcggc gtcacgggaa
     4021 ctgaggcgtt ggcaaatacc tttagtcttg atcccttagg atcggtgcaa tggcgatttg
     4081 tcatggcggc ggatcatccg ctggcgaacg ttgaagagcc gctaacagaa gcgcagttgc
     4141 gtcgctttcc ggcggtcaat attgaagaca gcgcccgcac cttaaccaaa cgcgtcgcct
     4201 ggcgattgcc agggcaaaaa gagattattg ttcctgatat ggaaacgaaa atcgccgccc
     4261 atctggcggg cgttggcatt ggttttttgc caaaatcgct ttgccagtca atgatcgata
     4321 atcaacaact ggtcagccgg gtaatcccaa cgatgcgccc tccttcgcca ttgagtctgg
     4381 catggcgcaa atttggcagc ggcaaagcgg tagaagatat tgtgaccttg tttacccagc
     4441 gcaggccgga aatcagcgga tttttagaaa ttttcggcaa cccacgcagt taaaaatcaa
     4501 gtttccttgc gcgttggcgc gtaaaatagc gcgcaagctg atttgaggtt cacttttaac
     4561 tatgcaagag agacacacgg aacaggacta tcgtgccctg ctgattgctg atacgcccat
     4621 tattgatgtt cgcgccccta tcgagtttga gcaaggcgca atgcccgccg ctatcaatct
     4681 gccgttaatg aataacgatg aacgcgccgc cgttggcacc tgctataaac agcaagggtc
     4741 agacgcggcg ctggcgctgg ggcataaact ggtggcgggt gaaattcgtc agcagcgcat
     4801 agacgcctgg cgggcagcgt gcctgcaaaa tccgcaaggt attctctgct gtgcccgtgg
     4861 cggtcaacgc tcacatattg tgcaaagctg gttgcatgca gcggggattg attatccgct
     4921 ggtggaaggc ggttataagg cactgcgcca gaccgcgatt caggctacta ttgaactggc
     4981 acaaaaaccg atagtgctga ttggcggttg taccggcagc ggtaaaacgc tgttagtgca
     5041 gcaacagccg aacggtgtcg atctggaagg gctggcgcgt catcgcggtt cggcgtttgg
     5101 tcgcacgtta caaccacaac ttagccaggc gagttttgaa aatctgctgg ctgccgaaat
     5161 gctaaaaacc gacgcccgtc aggatttgcg cctgtgggtg ctggaagatg aaagccggat
     5221 gatcggttcg aatcacctgc cggaatgtct gcgtgagcga atgactcagg cggcgattgc
     5281 ggtggtagaa gatccgtttg agatccgtct tgaacgcctg aacgaagagt atttcttgcg
     5341 tatgcatcat gattttaccc acgcgtacgg cgacgaacag ggctggcagg aatattgcga
     5401 atacctgcat cacggacttt cggctattaa gcgtcggctg gggttacagc gctataacga
     5461 actcgctgcc cagctggata cggcgctgac aacgcaactc accaccggca gcaccgacgg
     5521 tcatctggcc tggctggtgc cgttacttaa agaatattac gacccaatgt atcgctatca
     5581 gcttgagaaa aaagcggaaa aagttgtctt tcgcggtgag tgggcggaag tggcggaatg
     5641 ggttaaggcg cggtaatgag ggacgtattt gtattagtcg cgacttgatc tgacacatgg
     5701 ccttgaaagg ttgagagtta ccggttttga tatgagtatc gaatccttat ccaaaacaag
     5761 aggtaactct catgcttcat actgccaatc ccgtcatcaa acacaaagcc ggtttgctca
     5821 atctggctga agaactcagc aacgtatcaa aagcctgtaa aatcatgggc gtttcgcgtg
     5881 atacgtttta tcgttaccgc gaactggtcg ctgaaggcgg tgtggatgcg cagattaatc
     5941 gtagtcgccg cgctcctaac cttaagaacc gtaccgatga ggcaactgaa caggctgttg
     6001 ttgattacgc cgtcgctttc ccgacacatg gtcagcaccg ggccagcaac gagctgcgta
     6061 aacagggggg ttttatctcc gacagtggtg tccgttctgt ctggttactc cataaccttg
     6121 agaatctcaa aagacgatac tgaacaaatt ttatcaggtg acgttccgta aaaagttgta
     6181 tggagttctc gatgcagtcg gcgcgactat cagtaccccc gctgaaccct ggactatcgg
     6241 gccgccgtac tggggcatgt caccctggcg tgccgctggt tttccgctaa tggtttattt
     6301 cctctttctt tcccggcaag aatttatttt aatccggaag gattatttat tttcagatag
     6361 ggactcacat taaacacgta acattttaat taatgcatgg ctgaatatgt attacacaat
     6421 gtcagatatc gtaaaagacc agtgcaattc tatgtaaact ctctgactgt tcattttatt
     6481 tgttgtttca gggtcggaac tcaccctcaa taaacaactg aatacgagta tatattcatc
     6541 gtctgagcta tatggcttta cacaatagcc gacatgtctt ttctcagtca ggaattttac
     6601 ttttcctcac tacgaagttg acgacttcga tatgggatag actcttaatt caagcaatta
     6661 ctctgcacaa gcatcaggca gtccagctcc aatatttgtt ataaaataat gcgtaacatt
     6721 aatttcacca acaaaaaaag gtttacacca tccccttgcc ctggtatctt accatattta
     6781 caccacactt tttttgtatc tggatcattc tttgatacga ttttcgccac ttgttccctg
     6841 gccctcactg tcaatgtttt cttaaaccta ccctcctcat catttgttag ggaaaaataa
     6901 gaaaaaaaat aatcatcttc tacatttgtg tatattgata tatccaccgc tacgtgcgaa
     6961 aaaaaatctg ggagccatgt aaaaactcca tcattttctg gtattgccat atagtttatg
     7021 taatgtttac ttacaagaga ttcatctttg tataa
//
LOCUS       DAJTNA010000192         6997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTNA010000192 DAJTNA010000000
VERSION     DAJTNA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.194"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..230
                     /locus_tag="OO161_003497"
     CDS             <1..230
                     /locus_tag="OO161_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464434.1"
                     /translation="FTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLNKMA
                     ESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(199..1935)
                     /gene="aceK"
                     /locus_tag="OO161_003498"
     CDS             complement(199..1935)
                     /gene="aceK"
                     /locus_tag="OO161_003498"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR5464435.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLMTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCSEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2216..3520)
                     /gene="aceA"
                     /locus_tag="OO161_003499"
                     /pseudo
     CDS             complement(2216..3520)
                     /gene="aceA"
                     /locus_tag="OO161_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(3550..5151)
                     /gene="aceB"
                     /locus_tag="OO161_003500"
     CDS             complement(3550..5151)
                     /gene="aceB"
                     /locus_tag="OO161_003500"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR5464436.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAVWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            complement(5420..6349)
                     /gene="metA"
                     /locus_tag="OO161_003501"
     CDS             complement(5420..6349)
                     /gene="metA"
                     /locus_tag="OO161_003501"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5464437.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6506..6954
                     /locus_tag="OO161_003502"
                     /pseudo
     CDS             6506..6954
                     /locus_tag="OO161_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1685 a         1797 c         1816 g         1699 t
ORIGIN      
        1 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
       61 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
      121 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
      181 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
      241 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
      301 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
      361 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
      421 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
      481 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
      541 gacaccgaag tttttaaaca gcatgtcgcc aggaaaaatg ttagcagcgg caagctggcg
      601 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
      661 ccagatattg agcggcacca tccgccgctc gatatagaga tggcgaatta caatctgctc
      721 accaagatcg gtgatttttt ccgctgcttc ctggagcagt aattccatta atgccgggga
      781 aatatgccgc ttctccagca caaagttttc aaactcctgg gtgtccgcca ttcggcccac
      841 acggtcgtgc tctttcacca gttgatagca ggcacgaacg tgagcggcag acatctcttt
      901 ctgcggcgcg aacttgtctt tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa
      961 caccaacatc accattccac gaatacccgg cgcttcaatg aactgctcac tacagccctg
     1021 tagataaacg agatattcgc ggtagctttc ggttttggcg tgcttctggc agccgatagc
     1081 catatacaat tcagcagtgg ttttacctgg cagaatttcc cgtagccact cgaccagcgc
     1141 tgcgggcagc ggtgcataaa ccataaaata agaacgcgca aagccaaaaa caatgctcgc
     1201 ttcggcggtc gtcatcaggc aggtatcaat aaataactcg ccgtcgtccg tctggtggat
     1261 cggcagtaag aagggcaacg tgccggaagg cgtgatcagt ttgcccacca gccaggcggc
     1321 tttattgcgg taaaacagtt cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt
     1381 ccccagcgtt tccgtcagat ggcgaatgat gtaatgaatg tcacggcttt tattctgcca
     1441 gcgcaggcgc agcggcaggt cgctgataac gcgcatcagt agagattccc agccgtgatc
     1501 ggggtgaaag tctttcgcca gcgggcgggg aatggtacga aagcggcgct ctggctgaga
     1561 gctaaagata aaaagccgct cgggagtaag cgagcggtgg tcaaataacc gacagtacac
     1621 ggagttaaaa aagctctccg caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta
     1681 atgctcttta acgcgcagta aaaatgccgc gtccgtactt tggccgttag taatgcagcg
     1741 cagttgctcc acgaccagac caacgtgatg atcgtaaaga tggatacggt ttttcatcgc
     1801 cagctggaca gcatgccagt cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc
     1861 gaggaatcga ccatactgag cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc
     1921 caggccacgc ggcatttaac gccctcatca ggagcagaga attgccggat gcggcgtaaa
     1981 cgccttatcc agcctacgtt cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc
     2041 atcaggcatc gtgcaccgat tgccggatgc ggcgtaaatg ccttatccgg cctacgttcg
     2101 gcaacggctg taggcatgat aagacgcgca agcgtcgcat caggcatcgt gcaccgattg
     2161 ccggatgcgg cgtgaacgcc ttatccggcc tacagtcagc aacggttgtt gttgcttaga
     2221 actgcgattc ttcagtggag ccggtcagcg cggtgactga agacgtgccg ccctgaataa
     2281 tagtcgtcac tttatcgaag taacctgtac ccacttcctg ctggtgagat acgaaggtat
     2341 agccatcttt cgcggcggca aattccggct gctgcacttt ctcaacgtag tgcttcatac
     2401 cctcgcccta ggcataggcg tttgccaggt caaacatgtt gaaccacatg ctgtggatac
     2461 ctgccagggt gatgaactgg aacttgtagc ccatatccga cagctgctgc tggaagctgt
     2521 caatagtttt gtcgtcgagg tttttctgcc agttgaacga cggcgagcag ttataagcca
     2581 gcagtttgcc cggatatttc gcgtggatag cttgtgcaaa gcgacgcgcc agttccagat
     2641 ccggcgtgga ggtttcacac cagaccaggt cagcatatgg cgcatacgcc aggctacggc
     2701 tgatcgcttg ctcaatgccc gcatgagtac ggaagaagcc ttcactggta cgctcgccgg
     2761 taataaattc gctgtcatac gggtcgcaat cggaggtgat cagatccgct gcatcggcat
     2821 cggtacgagc aaccagtagg gttggaacgc ccgtcacgtc agctgccaga cgcgccgcga
     2881 ccagtttctg aatagcttct tgagttggca ccaaaacttt gccgcccatg tgaccgcatt
     2941 tcttcactga cgccagctga tcttcgaagt gaactgccgc tgcaccggct tcaatcatcg
     3001 ctttcatcag ttcaaaggca ttcaggacac cgccaaaacc ggcttccgca tcggcaacga
     3061 tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc aatgcccgcg gaccattgga
     3121 tctgatccgc acggcggaaa gtattgttga tccgctccac cacagctggc accgagtttg
     3181 ccggatagag cgactgatcc ggatacatgc tggccgccag gttagcgtcc gccgctacct
     3241 gccatcccga cagatagact gcttcaatac ccgctttcgc ctgttgcagc gcctgaccgc
     3301 cagtcagtgc accgaggctg ttgatgtagc cttttttcga ctcgccatgc agcagacgcc
     3361 acattctcgc cgcgcccagt tgcgccaggg tgcattcagg attgactgaa ccgcgtaatt
     3421 tcaccacatc ttccgcgctg tatgggcgag taatgccttc ccaacgcggt tgagtccact
     3481 ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc tccatagtta
     3541 tgtggtggtt tacgctaaca ggcagtagcc tggcagggtc aggaaatcaa ttaactcatc
     3601 ggaagtggtg atctgttcca tcaagcgtgc ggcatcgtca aaacgcccct gggagaaacg
     3661 ttcttcgccc agttcgctgg caatgacttt catctcttcg cccagcatct ggcggaacaa
     3721 ggctttagtt accggtttgc cattgctcaa cattttttga tgatggatcc actgccagat
     3781 tgaggtacgg gaaatttcag ccgtcgccgc atcttccatc aggccataaa tcggcacaca
     3841 gccgttgcca gagatccacg cttcgatgta ctgcacggct acgcgaatat ttgcgcgcat
     3901 accttcttcg gtgcgttcac cgtcacaagg tgccagtagc tgatcggcag taatcggcgc
     3961 gtcttgttcg cgcatcactt caagctgatt tttacgggag ccgagaatgt cgttgaatac
     4021 cgccattgcc gtatccgcaa ggcctgggtg agcgatccat gtgccatcgt gaccgttatt
     4081 ggcttccagc gatttatccg cttttacttt gttgagcacc tggttattgt gctcttcatc
     4141 tttgctcgga ataaacgccg ccatgccgcc catcgcaaaa gcaccgcgtt tatggcaggt
     4201 tttaatcaac aggcgtgagt aagcattcag gaatggttta tccatcgtca ctgcctgtct
     4261 gtctggcagg acgcgatcgg gatagttttt caacgttttg atatagctga agatgtaatc
     4321 ccaacgaccg cagttcagac caacaatatg gtcacgcagc gcgtgaagga tttcatccat
     4381 ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc cgcgcggcag
     4441 attaaagcga tcttctgcat agctgaagac ttcgctccac cagaccgctt cctgccagga
     4501 ctgggttttc ggcagataga aatagggacc actgcccttt gccaacagag cctgatagtt
     4561 gtggaagaaa tagagcgcaa aatcaaacag gctgccgggg attgcctcac cacgccaggt
     4621 gacatgtttt tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg ctggattggg
     4681 cttgagctgg taaattttgc ctgcttcatt ggtatagctg atggtaccgt taactgcatc
     4741 acgcaggtta atttgcccgt cgatcacttt gttccagtcc ggggccagtg aatcttcgaa
     4801 atcggccata aagactttca cattggcgtt gagcgcgttg atcaccatct tgcgctctac
     4861 cgggccagtt atctctacgc ggcggtcttc taagtccgca ggaatcccgc gaattttcca
     4921 atcagcatcg caaatggaag ctgtttccga aataaaatca ggcaacgttc cgttatcaat
     4981 atcttgctgc tgctgaatgc gcgctgccag aagtttattt cgttgtggcg taaaatgcgt
     5041 caccagctca gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct cctgctcgcc
     5101 atacggcctt gtgaaagcca gttcatcggt tgttgttgcc tgttcagtca tcgtgcagct
     5161 cctcgtcatg gatccgaaaa cttccccact gtgaacgaag gatcgttgtg cgcttttcgt
     5221 tcagcacaac taagactact catttaaaat gcaaaatcaa aaacaatttc cattttaatt
     5281 aaaaatactt gataacttat tgataacaat gtgaataaaa tttattcacc tcctgaggtg
     5341 cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt aaggtgctga atcgcttaac
     5401 gatcgactat cacagaagat taatccagcg ttggattcat gtgccgtaga tcgtatggcg
     5461 tgatctgata gacgtaatag ttgagccagt tggtaaacag caaattaccg tgactacgcc
     5521 agctcgctcg cggtgtattt tgcggatcat tgtgcgggaa atagttatac ggtacttccg
     5581 ggtctagtcc ggcttccaca tcgcggaaat attcctgcgc cagcgtttgc gcatcatatt
     5641 cgggatggcc cgtcacaaag gcaatgcgct tatctttgct ggcaaacaga tatgcatccc
     5701 cttcttccgt ctctgccaga atttccagat cggtgtaatc acgaatcaac gctgccggaa
     5761 agtcagcata gcgcgaatgt ggtgccagga atgaatcatc aaagccacgc gtcagaagcg
     5821 catgaggatg gagaatatga tgctcgtaaa cgccagagag tttgtcggtg cgagtttgct
     5881 taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc ccagcagaca aacagcgtcg
     5941 aggtgacgtg atctttcgac cactccagca cctgtttgat ctgcggccag taagcgacat
     6001 cattaaactc caccaggccc agcggcgcac cggttacaat cagaccgtca aagttctgtt
     6061 cctgaatatc ttcaaagtta cagtagaagt tgttcagatg ctctgcgggc gtgttgcgcg
     6121 attcacggga atcgatgcgc aacagctgaa tatcgacctg caaaggtgag tttgaaagca
     6181 ggcgcagaaa ctgattttca gtttcaatct tcttcggcat caggttaagg atcagaacct
     6241 taagtggacg aatttcctga ccagacgcac gagaagttgt catcacaaag acgttttctt
     6301 cacgcaagaa attgacggcg ggtagctcgt ccggcacacg aatcggcata acctgattac
     6361 ctcactacat acacttatac gtttggacat ccagatagct gaagataacc agaaaatttg
     6421 ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa ggaaaagact acgttagaat
     6481 ataagaatga ctaaaaggag agaaaatggt tattagtatt cgccgctcac ggcatgagga
     6541 aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat gccacacacg attttttatc
     6601 agcagagtat cgggccgagc tggaggtgct agctagttcg ttccttactg ccggaagcgc
     6661 cgttgtgggt cgcggttaat gaacgggatc agccggttgg gtttatgttg ttaagtgggc
     6721 agcatatgga tgcgctgttt atcgatcctg atgtgtgcgg ctgcggcgtg ggtcggatgc
     6781 tggtggagca tgcgctctcg atggcaccgg aactgacaac caacgttaat gagcaaaatg
     6841 agcaggcggt tgggttctat aagaaggtgg gttttaaggt tacgggacgc tctgaggtgg
     6901 acgatttggg gaaacccgta tccgttgctg aatctggcgt atgtggggga ataagccgcc
     6961 cctgccgtgc ctgcccaggc tggagaagac gcgccag
//
LOCUS       DAJTNA010000193         6890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTNA010000193 DAJTNA010000000
VERSION     DAJTNA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.195"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..906)
                     /gene="preA"
                     /locus_tag="OO161_003503"
     CDS             complement(<1..906)
                     /gene="preA"
                     /locus_tag="OO161_003503"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR5464438.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSH"
     gene            complement(900..2138)
                     /gene="preT"
                     /locus_tag="OO161_003504"
     CDS             complement(900..2138)
                     /gene="preT"
                     /locus_tag="OO161_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5464439.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSAQELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(2332..2571)
                     /locus_tag="OO161_003505"
     CDS             complement(2332..2571)
                     /locus_tag="OO161_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5464440.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNSGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(2574..3293)
                     /gene="sanA"
                     /locus_tag="OO161_003506"
     CDS             complement(2574..3293)
                     /gene="sanA"
                     /locus_tag="OO161_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5464441.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(3443..4327)
                     /gene="cdd"
                     /locus_tag="OO161_003507"
     CDS             complement(3443..4327)
                     /gene="cdd"
                     /locus_tag="OO161_003507"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5464442.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEITTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(4457..5152)
                     /locus_tag="OO161_003508"
     CDS             complement(4457..5152)
                     /locus_tag="OO161_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5464443.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(5149..5547)
                     /locus_tag="OO161_003509"
     CDS             complement(5149..5547)
                     /locus_tag="OO161_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5464444.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            5786..6736
                     /gene="dusC"
                     /locus_tag="OO161_003510"
     CDS             5786..6736
                     /gene="dusC"
                     /locus_tag="OO161_003510"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5464445.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSHDIARAIQAIDIDKLR"
BASE COUNT         1776 a         1791 c         1721 g         1602 t
ORIGIN      
        1 atgcgacaac ccgctcgcca tatcttctac gatgcgatag ccgtactgca taatgccggt
       61 ggtgacctgt aacgttgctg cgccgagcaa taaaaactcc gccgcatcct cccaggtttc
      121 aatgccgccg ataccgctga ttgggaaatc gcgcagttct ggatgggtgc gcatttgctg
      181 aatgaagcgc agggcgatcg gttttaccgc tttaccggaa tatccggaaa tactcgattt
      241 tccgttaacg atcggcatac cgattttctg attaagatcg atattggtga tggatttaac
      301 ggtgttaatc gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg ccacttcgca
      361 catatcaccg atattcggcg tcatcttcgc cagcattggc agcgtggaac cccgtttcac
      421 tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac ccatcgcatg
      481 agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag cttcttgcac
      541 caggcgcgcc agctcctccc attgctgctc attttccccc atgatcgaag cgatcaatac
      601 tttgtccggg taatcttcct tcagccgacg cagggcggcc agattctctt ccaacggatg
      661 ttcagcaatc tgctccatat ttttgaagcc gataaaaccg gtatcttctt tcaccagatg
      721 atcaaaacgc ggcgagactt cgttggcgat aaaaaagccg atcgttttaa acaccacacc
      781 gccccaacct gtgtcgtagg ctttggcaca catctcatag cagttgccta ccggcgaaga
      841 agagaggcag aacgggttgg gaaacttcac gccgcaaaaa gtaatcgaaa gatctttcgt
      901 taacatgagc aagctccctc taaatagtga tgaatcgcct cggcggcttc tttcccggtt
      961 ttcacggcat agaccacggt tttgtcaccc tcaacaatat cgcccgcagc aaagacttgc
     1021 ggatcgcggg tctggtaatt ttgtgtattg atagtgttac gctgcggctc taactccgca
     1081 aaggcatcca gtctggcatg ctgaccgacg gcgagaataa ttttatctgc cgccattgtc
     1141 agttcgcccg gtaaccgtac atgcttaaac gtgactttat tgccttccac ggctactggc
     1201 gtgaatccat cgatgatcga aacgcccagc tcttgggcgc tggtaaactc tttttcgctt
     1261 gccggaaact catctaactc ttcacgcgct acgcaagtta ccgcctgaca gccgagaact
     1321 ttcagcgtgc tggctacgtc catcgcgaca tcaccgccac cgataattaa tgcgctttgt
     1381 ggaatgctga tatcgccttg cgcctgtcgt acacgttgca ggaagtcgac ggcaatctca
     1441 acgtcgctat gctcaaacag cggtagcccg gaaccgcttg ataaccccac ggtgaccagt
     1501 accgcgcggt tttccgcttt cagttgctcc agggtgagtg ttttaccgat ttcgttattg
     1561 cacttaatgg tcacgcccat tttttcaata cgggcgatct ctgcatccag cactgattgc
     1621 ggtaaccgga attgcggaat accgttacgc aaccagccac cggggtgcgc ttctttctca
     1681 taaatcgtga cgtcataacc ctggtttgtc agtgtcacac tggcctgcaa tccggcagga
     1741 cctgcgccaa taatcgcgac tttgccgagc gttttagtac cgggctgata aatttccatc
     1801 ccggtttgtt gttcaaaatc agtaacaaaa cgctgtaagc ggccgatatc aatgggagca
     1861 tcaacaccgg cacgggtaca accgctttga cataatttct ccgtcgggca cactctggca
     1921 caaacggcac cgagggcatt attttcgcga attgtctcgg cagcgccttt aaaattacga
     1981 aagtagattg agcgaataaa tttccccgga tcggtctgtg ctgggcaagc ctgactacag
     2041 ggagcgtcat gacataataa acagcgagag gcttctttaa tcgccagtaa aggcgtaaaa
     2101 gccggagtga gttcatccag ataattttgt tgcggcatgt gatcttcctt aatgctatga
     2161 gtaattaagg cgaacgctaa ataaatattg cgtgctgata ttaaggctat gcgcgtgggg
     2221 aaatttaaac tgtgaaagga ttcacaaata gctgcggttc agaatgttat atccgcagct
     2281 tattgagtga agtaataatt attctcaata attaacagaa aagagaaaat attaacgata
     2341 gaaaataaga taaacaatca tgccgataga taaaattccg aagccgatat atgccaccat
     2401 ataagcgaaa aagagtccgg aattttttgc tcgccagaca tacaccggtt ccggtgcatt
     2461 tttaacccgt ttatcaatcg cgcctgcgcg ccagcctttc catgcttcgc ggaaacaaaa
     2521 tatcgggata aggaaaaaaa cggcaacgac aaaaaactgc tgtacatcca tagctacttt
     2581 ccttgtttct tttgtaattc aagtaactgt tcgggtgtga cggcgggata cccctgcgca
     2641 tcttccggca cctggtgcat agccggaata gggaccagcg gccctaaaaa acgcggttca
     2701 cgtttaaaaa tataaaggtc agccagcgca ccgaaacggg cggcaaattc gcgaatacgt
     2761 actgacagca tatctttcgg tgacggtacg gcataacact gagcctgaat ccccatatgc
     2821 agcgcaataa ataatgcccg ctcacagtgg aaacgttggg tgataataat gaaatcatta
     2881 gtatcgaaaa ctttgcgtgt acgcactatg gaatccagcg tacgaaagcc tgcgtaatcg
     2941 agaacaatat ctgatgggtc gacaccagca gcgattaaat ctttgcgcat ggtcatcggc
     3001 tcattataac tttgcaatgc gttatcgccg ctcagtaata gataatttac cttaccgctg
     3061 ttataggcat taatcgctcc ttgaatgcgg tagcgataat actgattaat tacgccagta
     3121 cgataatatt ttgctgttcc gagcaccaca ccgacctggc ggtaggggag atcctgcaat
     3181 tcgtcgtaga tataaggcgc ggttttccag ctcatccagc gatcgaggcc gagcacagtc
     3241 aacagcagca agccgataag gactaacagg ctgaggaaca cgcgctttaa catgcaggag
     3301 actcgatcta agggaaattt ccatcaggct actttacccg ctggttttgc gcaagaaacg
     3361 gcaaactgtt tgccgggatt ttcgacacca ggagagggct ggcaagccgg acaaggcgtt
     3421 cacgccgcat ccggcaccag gcttaagcga gaagcacgcg gtcgatactg tgacagccga
     3481 gcgctttcag cgttgcggag gtggcatccc actgaatcaa cggcgcatcg gctttttctg
     3541 ccagaaccgc gcgctggata tccgggtaat cataaccctt gagattcaac agaattaacg
     3601 ctccctgcaa cggtggcaga gtcgggttga atgcggcgtt ttcagcgtag ctgccgctga
     3661 aaatacgacc gtctttacat tccagcgcga caccgcttgg cgacttactg taaggcatgt
     3721 gcgaacggtt tgccgccgca atcgccgcct gagaaagcgc atcaccagtc agcgcatagc
     3781 cgtgatcctg ctcgtccatc agcagcgttg taatctccag atctttcggc ccaaaggcat
     3841 ccggcagata gtcacgtagc gcgtgtgcct cgcggcccgg cagatgaata cgcagatcca
     3901 gaccgctgtt cagttcattc ataaactgac ggcagtgacc acaaggcgtg tagttaacgg
     3961 tgatggctgc aagcgctttt tcaccactca accaggcgtg gctgatcgcg ctttgttcgg
     4021 catgaacggt ttgctgcatt gtcgcaccaa taaattccat attggcacca aaataccagg
     4081 ttccgctcac accgcgcgca attgcgccaa cattaaaatt cgacaatggc gtacgcgcac
     4141 aggccgccgc cagcggaagt agtgcgaatg ccagcgcgtc ttcgtccagc cccgttgcgc
     4201 tcttcagcga tgagacttgc tccccggtca acaaagcggg gaagtacttg tctgccagaa
     4261 taggttccag tgcagattgc aagttatccg caagttgggc aaaagcggtt tgaaaacgtg
     4321 gatgcatggc gttgcctcat aatcatgtaa tgggataaca gtttacgaac ccgttatggc
     4381 tttatatgtg atctgaatct cattaattat gcaacgcgtg aaacttacat caaaaatgcg
     4441 cgacgcatcg caaattttag cccattactg ccagaataat cgggaaaagg aacggtgcga
     4501 tcagcgaagt aattatcccg cataacacca gcgctagcga actaaatgca ccttcctgat
     4561 aatccagctc ggcgcagcgc gctgtaccga gggcgtgcga ggcagtcccc attgccagac
     4621 cgcgcgcagc tttggtacga atacgcatcg cattaagcaa tgtatggcca aataccgcgc
     4681 cgagaatgcc gacgaaaatc acgcaaactg cgctgattgc cggaataccg ccaatactgc
     4741 cgccaaccgc cattgcaata ggcgtggtga ctgatttcgg caggattgac gcggcgattt
     4801 ccggtgaagc gcccatcaac aatgccacgg aagtccccgt caccatcgca accacgctgc
     4861 cgataaaaca gatggtgatg atcgatttcc agcgcgcgcg gatctggtgt agctgctcat
     4921 ataaaggata ggccagcgcg acgaccgccg gttgcagcag atcgtttagc acttcgctac
     4981 ctttaaagta actgtcgtaa gagatgccgg tcagcattaa aaaaggaatg atcaccacca
     5041 ttgctaccag caacgggtta agcaggggaa acttataccg tgccgccagt ttgcgggcgg
     5101 caaaaaagac aatcaacgtc agcggtaatg accaccagat attcgccatc attcttctga
     5161 tcctttctga cctacgactt tacgttcgcc gtgtaccagt tgtgaactcc agctcaccac
     5221 cagaaaaacc accagcgtac tgacagcaca ggaaaccact accgggccaa actgcgcgcg
     5281 gagcaaatca aaatattgca tgacgcctac gccaatcggc acaaacaata gcgccatata
     5341 gcgaatcagt acgtagcacc ccggattgac ccattttgcc ggaagaattt gcaaggccag
     5401 caggacaaac aggatcagca tcccgatgat gctgcccgga atggttaccg gtagcaggga
     5461 agcaatgaaa atgcctgcat acaggcaggc ataaatcagg acgaaagcgc gtaaatattg
     5521 ccagataatg ttcagtgtct tactcataat caacacccca gatgagatgg attcatcata
     5581 caattaatcc gtaaaacgtg ctaccgatca cattaagaga tcagcgctta ttttttcgct
     5641 ccccggcatt acgcaatgcg caacgaactc aggcatacat caagcataga gagggattac
     5701 agtttgcagc aatccctctt tgcagacctt taatccgttg accataggcg ctaacatggc
     5761 gcctcatttt tttgcgggtg aagatatgcg tgtgttactg gctccgatgg agggtgtgct
     5821 tgactctctg gtgcgtgaat tgctgaccga agttaacgac tacgatctgt gcatcaccga
     5881 gtttgtccgc gtggtggatc aactgctgcc ggtaaaagtt tttcatcgca tttgccctga
     5941 gctacaaaac gccagccgga caccatccgg tacgctggtg cgcgtgcagt tgttgggtca
     6001 gttcccacaa tggctggcag agaacgccgc ccgtgcggtg gaattaggtt cctggggcgt
     6061 ggatctcaat tgcggctgcc cgtcgaaaac ggtcaacggt agcggtggtg gggcgacgtt
     6121 actcaaagat cctgaactca tctaccaggg tgcaaaagcg atgcgtgaag ctgtaccggc
     6181 gcatttgccc gtcagcgtga aagtgcgtct gggctgggac agcggtgaga agaagtttga
     6241 aatcgccgat gcggttcaac aggctggtgc tacggagctg gtggtgcatg gacggacaaa
     6301 agagcagggt taccgcgcgg agcatattga ctggcaggcg attggcgaga ttcgccagcg
     6361 gctgaatatt ccggtgattg ccaacggcga aatctgggac tggcagagtg cgcaacaatg
     6421 catggcgatc agcggctgcg acgcagtgat gattggtcgc ggggcgctca atattcccaa
     6481 cctgagccgg gtggtaaaat ataacgaacc gcgaatgccg tggccggagg tggttgcttt
     6541 gctgcaaaaa tatacccgtc tggaaaagca gggcgatacc gggttatatc acgtagcgcg
     6601 gattaaacag tggttgagtt atttgcgtaa agaatacgat gaagcaacag aattatttca
     6661 gcatgttcgg gtgttgaata attcccatga tattgcaagg gctattcagg caattgatat
     6721 cgataagctg cgataatagt ggaatcgcag cttatcagag acaatgaatc gcttattcag
     6781 cttcatcatc ttgttgcgct cctttcatga actaagccac atattgccac tggcgcaagg
     6841 tgcgcgcaga gggaggacaa tcgcctcctc cccctgcacc cccgggctct
//
LOCUS       DAJTNA010000194         6836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTNA010000194 DAJTNA010000000
VERSION     DAJTNA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.196"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..767)
                     /gene="uxuR"
                     /locus_tag="OO161_003511"
     CDS             complement(<1..767)
                     /gene="uxuR"
                     /locus_tag="OO161_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5464446.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKV"
     gene            complement(982..2442)
                     /locus_tag="OO161_003512"
     CDS             complement(982..2442)
                     /locus_tag="OO161_003512"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5464447.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLKAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2523..3707)
                     /gene="uxuA"
                     /locus_tag="OO161_003513"
     CDS             complement(2523..3707)
                     /gene="uxuA"
                     /locus_tag="OO161_003513"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5464448.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4047..4251
                     /locus_tag="OO161_003514"
                     /pseudo
     CDS             4047..4251
                     /locus_tag="OO161_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4494..5396)
                     /gene="fimH"
                     /locus_tag="OO161_003515"
                     /pseudo
     CDS             complement(4494..5396)
                     /gene="fimH"
                     /locus_tag="OO161_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
     gene            complement(5416..5919)
                     /gene="fimG"
                     /locus_tag="OO161_003516"
     CDS             complement(5416..5919)
                     /gene="fimG"
                     /locus_tag="OO161_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5464449.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(5932..6462)
                     /gene="fimF"
                     /locus_tag="OO161_003517"
     CDS             complement(5932..6462)
                     /gene="fimF"
                     /locus_tag="OO161_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5464450.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(6472..>6836)
                     /locus_tag="OO161_003518"
     CDS             complement(6472..>6836)
                     /locus_tag="OO161_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5464451.1"
                     /translation="ALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTL
                     THNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCIANYQ
                     LPPESQQQLLTQLSAECR"
BASE COUNT         1592 a         1852 c         1731 g         1661 t
ORIGIN      
        1 tcgactttat ccagcggcca tgaatcaaac agatagccgt caaaataaat atcgtcaacg
       61 ttcgagaatt ccagcagacg ttgcttaacg ttttccagat gctgccacat tgccagcttc
      121 gccgctcggg catctttttt gattaacgcg gcaaggatct gtttgtgatc gcccaaccac
      181 tctttgcgat acaggctgtc atccagatgg ctgtgcaact ggatccacat tggattgttt
      241 tcccgccact gccaggactg acggaataac tccaccagca tgctgttatg cgttgcttca
      301 gcaatggcga gatggaactg catgtcaccg ctttcgctgc tacccggcgc gctggaagcc
      361 agttcacgct cttccagttg caatgcctga cgcattttga cgatatcttc gcgggtagcc
      421 tgcagggcgg caaattccgc gatgttgctc tcaagaagct gtcgcgcctg caacaattca
      481 aaggggccgg catcattgca gacgttggca tccggactgt ctgtgttgtg gctgcctgag
      541 ctatcaagaa catagatacc ggcaccccgg cgcacttcca ccagcccttt gatctccagc
      601 atgatcagcg cttcacgtac caccgtccgc gtgacatcaa gcatttctgc aatttcacgc
      661 tccggcggca gccgttcgcc aggattgtac ggcgtcttta tgatcagatc gcggatcatc
      721 gccccgactt cctggtaagg tctttgcgca gaggtggcag atttcatggt gttgtgtccg
      781 ttaggtgcgg ttaatcaaat ttttgccgga tgcggcgtga acgccttatc cggcctacaa
      841 cgggcaattt tacggccccc gttaatctca aactatacca ttagcactgg aagttggcac
      901 gtacaggtgg gcaaaaaaac gaaaactggt caaccaaata tcagtttgat tgaccagtaa
      961 gtctgtaggg taatcagtta gttacagcgc agccacacac tcgcgcgcac cgcgttcgca
     1021 cagctgctga tatgccgccg tcaccgcgcc aacaaagtcg gcattctgcg gcagatcatc
     1081 ggcaaaaata ccgctcaggc ccagcagcgc tttcacgcgg tctgcgccct gatactgcgc
     1141 gttaatcttc tggaactcgg ccagcatcgg gtcgaccacg tcaatggcat taccctgctc
     1201 atccacgccc tgggtgtaac gcatccagcc agccacgccc agcgccaggt gacgccagct
     1261 gccgccgttt tgcaggtgca gacgcaccgg gtccagcaga cgctgcggta acttctggct
     1321 gccgtccatc gcaatctgcc aggtacggtg acgcagagac gggttgctga aacgctcgat
     1381 cagcagcgtc gcataggcct tcaggtccgt accctccggc atagacagcg ttggcgcttg
     1441 ttcctgcatc atcagggcaa aggccgcttt gcgataatcc gggttagtca tggtgtcggc
     1501 aatagtttca tagccgccga ggtaaccgag gtatgccagg aaagagtggc tgccgttcag
     1561 catacgcagc ttcatcattt cgaacggcac aacgtctgca acgaactgtg cgcccacttt
     1621 atcccagtcc gggcgaccat taacgaagtt atcttcaatc acccactgac ggaacggttc
     1681 gcaggcaatg gcgcacgggt cgtaaacacc cagctggtca gcaatttcct gtaaggtttc
     1741 tggcgtcgcc gccggaacga tgcggtcaac catggtgcac gggaaggtga cgttctcttc
     1801 aatccatgcc gccagctgcg ggtcacgtgc ctgagccagc cccagtaccg cgaccttcgc
     1861 cacatgaccg ttttcacgca cgttatcgca ggacatcacc gtaaatgctt tcagcccttt
     1921 ttcacgacgc agacgcaagg cttcgacgat gtaaccaatc gcggacttcg gcgcagtcgg
     1981 gttttctaga tcgtgcttga tcagcgggtt attgagatcc agttgaccgc ttgccgcatc
     2041 agcgcagtag cctttttccg tgaccgttag agagacaatc gccgtttgcg gacgcgccat
     2101 cgcgttgaga ataccttcgc agccgtcgat ttccggatgc agcgcttctt tcatcgaacc
     2161 gataattttc agctcggtgc tttctgcgcc tttttccgct acggtgtaca gcagttgctg
     2221 ttttttcagg ttttcgatca gcacgcggtc gttgcctggc atcaggttaa cttcgcagat
     2281 gccccagtcg ctgtcggtgc tttccagcag atgatgggta tacagcgcct gatgtgcgcg
     2341 gtgaaacgcc ccgcaaccga gatgcacaat gcgtgattcc agacgagaat gatcccatga
     2401 cgggcgggca accggcagat tgctgtcaac aatagtagtc atcgtaaact ccagaatccg
     2461 tcttcagaga gtgacggcaa ggaagaagga accggcacgg cgcgcagcca tgccggtgga
     2521 tattaacggc taaagaaagc gcgctggatc gccagttcga caccgcgaac ttcggccagg
     2581 cctttcagac gaccaattgc ggagtaacct gggttggttt tcttcttcag gtcgtccagc
     2641 atctgatgac cgtggtccgg acgcatcggg atcaggtctt ctttgccttc cgctttacga
     2701 cggtgttctt cttcaacaat cgctttcacc acttcgtaca tatcaacgtc accgttcagg
     2761 tgcgccgctt catggaaggt tttcgggtta tcttcacgca tggtggagcg cagatgggtg
     2821 aagtaaatac gcggaccgaa ctgcttgatc atatcaacca gatcgttgtc agcacgcacg
     2881 ccgtaggaac cggtgcacat ggtaaaaccg ttcgccatgc tgtttacggt atcaaccatc
     2941 cactgcatat cttcaatggt ggaaacaatg cgcggcaggc cgaggatcgg gcgcggcgga
     3001 tcgtccgggt gaaccgccat acgcacgcca acttcttcag caactggaat aatcgctttc
     3061 aggaagacgg caaagttttc gcgcagcttc gctttatcga tatctttgta cagctccagg
     3121 tgcttacgga actggtcgag ggtataccct tcttccgcgc ccggcagacc ggcaatgatg
     3181 ttacgcgtca gacgcgcttt gtcttcatcg ctcatagtgg cgaagcgttc agctgcctga
     3241 gcaatttctt cttcggtgta atccgcttcc gcgcctggac gtttcaggat atgcatttcg
     3301 aatgcagcga attcgatctg gtcgaagcgc agagctttgg agccgtctgg cagcacgtat
     3361 tcgaggtcag tacgggtcca gtcgagcacc ggcatgaagt tgtagcacac ggtgcgaatg
     3421 ccgcactgcg ccaggttgcg cagagtctgc tgatagttag cgatccactg ctcatagttg
     3481 ccagtgtggg ttttgatatc ttcgtgaatt ggtacgcttt ctacgacaga ccacaccagg
     3541 cctgcgtctt caacgatcgc cttgcgtttg aggatctctt ctacggacca tacttcgccg
     3601 ttcgggatat ggtgcagcgc ggtaaccacg ccagttgcgc ccgcctgacg gacatcagct
     3661 aaagaaacca gatcgtttgg gccgtaccag cgccaggtct gttccatgat ttttcctctt
     3721 tcacatagtg ggttccacag caaagagacg cggaaccctg gagttgttta ggtatccgga
     3781 tgaacaacca gaccgcagcc gtggcgttgc atcggatgag atggcgtata agttctacct
     3841 tttggtaaga tatgtcaacc agaattctga aattggttaa ccaaatcaca agaatttcac
     3901 tctcatggtt gttgctgcaa attgcagcaa aaggtaatcg atggttattg atcagtggat
     3961 gtgacattca tcgcaacaat ggttgaccaa tttacataac atgtcgccca aaataacaac
     4021 ggttcaacca ggtaaggtaa aacgttatgc atgtgcttaa cattctctgg gtagtattcg
     4081 gcattggtct gatgctggta ctgacaacga ccgcaggttc agacacattc actgcattag
     4141 caatgcctct tagattattt tgacctgtca gtaaaagaca cgttgaaaac ctggggtctg
     4201 ctggagctgg tcaactccgt ggttgggctg attattagca aggtagcgta aaagaacacc
     4261 agagcctgcc aatggcaggc tctgactgat gacaaagtct aaaaatgtgc ccggacagtc
     4321 ccctcgcccc tccggggaga gggttagggt gaggggaaca gaccagcaaa gtgcgctttg
     4381 tttatgccag atgcgacgct gacgcgtctt atctggccta caaagggcta acgtgcaggt
     4441 ttttagcttc aggtaatatt gcgtaactgc attagcaatg ccctgtgatt tctttattga
     4501 taaacaaaag tcacgccaat aatcgattgc acattccctg cagtcacctg ccctccggta
     4561 cgtgcgtaat ttgccgttaa tcccagactt accgccgaag ttcctactgc tcctaacgat
     4621 accgtgttat tcgctggaat aatcgtaccg ttgcgcgtca actgtacgcc gacgccctgc
     4681 gctggtgaaa acgacgcggt attagtgaaa atcgagttgc ccgcatctgc agttgtgccg
     4741 gagaggtaat accccaggtt ttggcttttc gcacaataaa cggtaagagg aattggcact
     4801 gaaccagggt agtccggcag agtaacggtg acatcacgag cagaaacatc gcagccgcca
     4861 gtaggcacca ccacatcatt attggcgtaa atattccaca caaactggaa atcatcgctg
     4921 ttatagttgt tggtctgtcg caaaataagc acggcaatta atgagccagc tttaatcgcc
     4981 accccgcccg cactgctcac aggcgtcaaa taaagcgcca ccggccacgg cttatccgtt
     5041 ctcgaattat aaacaacccg cggcgtttcg ctggtggtcg gaaatggata gctactgcca
     5101 ctatatttta cggtcccgga aaaattagat aacacgccgc cataagccga gcctcgttgc
     5161 agtgtgacat agtctgtaat ggtttccgga taatcgttat ggcaaaagat ttgcgtcgaa
     5221 agatctacga ccaggttttg ccccacattc acgacaggcg caaggtttac ataaacatta
     5281 gcgctgccac cgccaatagg gatagcagta ccattggcgt ttttacaggc gaatgactag
     5341 gcatttaccg accagcccat cagcagtaca gcaaacaggg taataactcg tttcattaca
     5401 attatctctt cgggttcagc tgtaggtata ggtgatgcta atcactgcct gaatggttcc
     5461 ctgagtggct ccgccattta ccgtcaatgc tctgacctgt aacgggaagt gcgctgattg
     5521 tgaggaatca tccacctgaa ctgttttggt tgcgccagta ttcaatgtgt tgccactgtc
     5581 atcctgtagc tctaactgga tgttttgcgc ggtcccctgg tttttataat atccggtact
     5641 gtcggctgcc ccgctgaagc tggcagtgac cctcgacgtt cccaccggac aattagtcaa
     5701 ctcaagcgca acatcatgcc aggccgatgc cgccccggca gacatcagac tgaaagaata
     5761 aagatcgccg agatcaaccg tggcattggt ggtggaaact gtgcacggtt tggcgacgac
     5821 cttaccgttc accgtgatgg tgacatcggc tgcctgtatt gtcgcacttg cgagcgccaa
     5881 tattgccgcc aataaatacc cacgtttgcg ccatttcatg agcatctcca gttactgata
     5941 ttcaagagtg aaggtagccg tggcattgat atgccccgca gtgacaggca cctgtgtcgc
     6001 cattagccgg gcgtaaaaat tcaacgtatt tggtttaccc ggcgtcaggg tcgtccacga
     6061 aatcgcggac gatggagcat taaggggtat ctgattttgc tgctcattca gaagctgtat
     6121 tcccagtccc gaagccgctg acaccgtatt ttcaagtgca agcaggttgg cattgtggct
     6181 atctgcaacg ccggtaaacc caacctttac ggcagaaacg gcgttaccac agggtgacag
     6241 caaaatacga aatggaacga caggagtcgt tgcgccaatg ttgttaaatt gcttcgccgc
     6301 gttttccatc agatcaacag taaaattggt tgattcagcg gccacactac agccgttatc
     6361 tctgacatag ccgcggatag taatcgtgct atccgcagcc aaaacgcgac ttaccgccag
     6421 ccacaaaaaa gcgcacagaa gataaaaagg tttgtttctc atcacgcccc cttaacgaca
     6481 ttcagctgat agctgggtta ataactgctg ctgactctct ggtggcagtt gataattggc
     6541 gatacagtga gcattttcct cttctcccca tttcacctga acttttcccg ctaaaggcat
     6601 tccgctgagg taaacctgac cattatccgc aacaatgccg ctactctggc tactctctga
     6661 tgtcaccatc gccccaaacg gcagcggctt attattgtgg gttagcgtca tgagcagttt
     6721 tatcccaacg cgcgctttaa actctgctcg cacgatcgcc ccacgagtgg gaacaacgtt
     6781 agcgaccgcg ttatctaaat cgacgttatc agccagggta ttggtatcca gcgcca
//
LOCUS       DAJTNA010000195         6701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTNA010000195 DAJTNA010000000
VERSION     DAJTNA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.197"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..166)
                     /locus_tag="OO161_003519"
     CDS             complement(<1..166)
                     /locus_tag="OO161_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="YhcL; uncharacterized member of the DcuC family of
                     anaerobic dicarboxylate transporters; probable role in
                     transport of carboxylates across the inner membrane from
                     the periplasm; its encoding gene is not essential for
                     growth and is probably cryptic; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter"
                     /protein_id="HCR5464452.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSG"
     gene            547..1338
                     /gene="nanR"
                     /locus_tag="OO161_003520"
     CDS             547..1338
                     /gene="nanR"
                     /locus_tag="OO161_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5464453.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            1460..2353
                     /gene="nanA"
                     /locus_tag="OO161_003521"
     CDS             1460..2353
                     /gene="nanA"
                     /locus_tag="OO161_003521"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5464454.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            2462..3952
                     /gene="nanT"
                     /locus_tag="OO161_003522"
     CDS             2462..3952
                     /gene="nanT"
                     /locus_tag="OO161_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5464455.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVYILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            4000..4689
                     /locus_tag="OO161_003523"
     CDS             4000..4689
                     /locus_tag="OO161_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5464456.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            4686..5561
                     /gene="nanK"
                     /locus_tag="OO161_003524"
     CDS             4686..5561
                     /gene="nanK"
                     /locus_tag="OO161_003524"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5464457.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            5558..6022
                     /locus_tag="OO161_003525"
     CDS             5558..6022
                     /locus_tag="OO161_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5464458.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPSEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(6098..6403)
                     /locus_tag="OO161_003526"
     CDS             complement(6098..6403)
                     /locus_tag="OO161_003526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5464459.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(6420..6701)
                     /locus_tag="OO161_003527"
                     /pseudo
     CDS             complement(6420..6701)
                     /locus_tag="OO161_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1558 a         1654 c         1787 g         1702 t
ORIGIN      
        1 agccgctgct tttggcggga tcgagtacac caccgaaccc taaccagaca ccgcacatca
       61 tcaggaagat acctgcggca gccagcacca cctgaggttt gtagtttttc aggatcaaat
      121 agcccatcgt aattaatacg atgacagata taattatgcc gaacattgtt gaactccgtg
      181 tcaaaagaaa actgtcaatc ccataaacgg cagattgaaa acaatgatgt tatatttttt
      241 gcaagtctat ttatggtgcg gatgtcgtgt ttttaattgt aggtgaggta atttttcatt
      301 aaaaaaacat gcgcttatga ttattttgca agaacgcatt cataatattc ataatgctcg
      361 tgaatagtct tataaataat tcaaacggga tgtttttatc tgcgttacat taatttttcg
      421 caatagttaa ttattccgtt aattatggta atgatgaggc acaaagagaa aaacctgcca
      481 ttttccccta ctttcaatcc tgtgatagga tgtcactgat gatgttaatc acactgacct
      541 tacagaatgg gccttatgaa cgcatttgat tcgcaaaccg aagattcttc acctgcaatt
      601 ggtcgcaact tgcgtagccg cccgctggcg cgtaaaaaac tctccgaaat ggtggaagaa
      661 gagctggaac agatgatccg ccgtcgtgaa tttggcgaag gtgaacaatt accgtctgaa
      721 cgcgaactga tggcgttctt taacgtcggg cgtccttcgg tgcgtgaagc gctggcagcg
      781 ttaaaacgca aaggtctggt gcaaataaac aacggcgaac gcgctcgcgt ctcgcgtcct
      841 tctgcggaca ctatcatcgg tgagctttcc ggcatggcga aagatttcct ttctcatccc
      901 ggtgggattg cccatttcga acaattacgt ctgttctttg aatccagtct ggtgcgctat
      961 gcggctgaac atgccaccga tgagcaaatc gatttgctgg caaaagcact ggaaatcaac
     1021 agtcagtcgc tggataacaa cgcggcattc attcgttcag acgttgattt ccaccgcgtg
     1081 ctggcggaga tccccggtaa cccaatcttc atggcgatcc acgttgccct gctcgactgg
     1141 cttattgccg cacgcccaac ggttaccgat caggcactgc acgaacataa caacgttagt
     1201 tatcaacagc atattgcgat cgttgatgcg atccgccgtc atgatcctga cgaagccgat
     1261 cgtgcgttgc aatcgcatct caacagcgtc tctgctacct ggcacgcttt cggtcagacc
     1321 accaacaaaa agaaataatg ccactttagt gaagcagatc gcattataag ctttctgtat
     1381 ggggtgttgc ttaattgatc tggtataaca ggtataaagg tatatcgttt atcagacaag
     1441 catcacttca gaggtattta tggcaacgaa tttacgtggc gtaatggctg cactcctgac
     1501 tccttttgac caacaacaag cactggataa agcgagtctg cgtcgcctgg ttcagttcaa
     1561 tattcagcag ggcatcgacg gtttatacgt gggtggttcg accggcgagg cctttgtaca
     1621 aagcctttcc gagcgtgaac aggtactgga aatcgtcgcc gaagaggcga aaggtaagat
     1681 taaactcatc gcccacgtcg gttgcgtcag caccgccgaa agccaacaac ttgcggcatc
     1741 ggctaaacgt tatggcttcg atgccgtctc cgccgtcacg ccgttctact atcctttcag
     1801 ctttgaagaa cactgcgatc actatcgggc aattattgat tcggcggatg gtttgccgat
     1861 ggtggtgtac aacattccag ccctgagtgg ggtaaaactg accctggatc agatcaacac
     1921 acttgttaca ttgcctggcg taggtgcgct gaaacagacc tctggcgatc tctatcagat
     1981 ggagcagatc cgtcgtgaac atcctgatct tgtgctctat aacggttacg acgaaatctt
     2041 cgcctctggt ctgctggcgg gcgctgatgg tggtatcggt agtacctaca acatcatggg
     2101 ctggcgctat caggggatcg ttaaggcgct gaaagaaggc gatatccaga ccgcgcagaa
     2161 actgcaaact gaatgcaata aagtcattga tttactgatc aaaacgggcg tattccgcgg
     2221 cctgaaaact gtcctccatt atatggatgt cgtttctgtg ccgctgtgcc gcaaaccgtt
     2281 tggaccggta gatgaaaaat atctgccaga actgaaggcg ctggcccagc agttgatgca
     2341 agagcgcggg tgagttgttt cccctcgctc gcccctaccg ggtgagggga aataaacgca
     2401 tctgtaccct acaattttta taccaaagcg tgtgggcatc gcccaccgcg ggagactcac
     2461 aatgagtact acaacccaga atatcccgtg gtatcgccat ctcaaccgtg cacaatggcg
     2521 cgcattttcc gctgcctggt tgggatatct gcttgacggt tttgatttcg ttttaatcgc
     2581 cctggtactc accgaagtac agggtgaatt cgggctgacg acggtgcagg cggcaagtct
     2641 gatctccgca gcctttatct ctcgctggtt cggcggcctg atgctcggcg ctatgggtga
     2701 ccgctacggg cgtcgtctgg caatggtcac cagcatcgtt ctcttctcgg ccgggacgct
     2761 ggcctgcggc tttgcgccag gctacatcac catgtttatc gcgcgtctgg tcatcggcat
     2821 ggggatggcg ggtgaatacg gttccagcgc cacctatgtc attgaaagct ggccaaaaca
     2881 tctgcgtaac aaagccagtg gttttctgat ttcaggcttc tctgtggggg ccgtcgttgc
     2941 cgctcaggtc tatagcctgg tggttccggt ctggggctgg cgtgcgctgt tctttatcgg
     3001 cattttgcct atcatctttg ctctctggct gcgtaaaaac atcccggaag cggaagactg
     3061 gaaagagaaa catgcaggta aagcaccagt acgcacgatg gtatatattc tctaccgtgg
     3121 tgaacatcgc attgccaata tcgtaatgac actggcggcg gctactgcgc tgtggttttg
     3181 cttcgccggt aacctgcaaa atgccgcgat cgtcgctgtt cttgggctgt tatgcgccgc
     3241 aatctttatc agctttatgg tgcaaagtac gggtaaacgc tggccaacgg gcgtaatgct
     3301 gatggtggtc gtgttgtttg ctttcctcta ctcatggccg attcaggcgc tgctgccaac
     3361 gtatctgaaa accgatctgg cttataaccc gcatactgtc gccaatgtgc tgttctttag
     3421 cggctttggg gctgcggtgg ggtgctgtgt cggcggcttc ctcggtgact ggctgggaac
     3481 ccgcaaagcc tacgtttgta gcctgttggc ctcgcagctg ttgattattc cggtatttgc
     3541 gattggcggc gcaaacgtct gggtgctcgg tctgttactg ttcttccagc aaatgcttgg
     3601 acaagggatc gccgggatct taccgaaact gattggcggt tatttcgata ccgaccagcg
     3661 tgcagcgggc ctgggcttta cctacaacgt tggcgcattg ggcggcgcac tggccccaat
     3721 catcggcgcg ttgatcgctc aacgtctgga tctgggtact gcgctggcat cgctatcgtt
     3781 cagtctgaca tttgtggtga tcctgctgat tggtctggat atgccttccc gcgttcagcg
     3841 ttggctgcgc ccggaagcat tgcgtactca tgacgctatc gacggcaaac catttagcgg
     3901 tgccgtgcca tttggcagcg ccaaaaacga tttagtcaaa accaaaagtt aatcctgttg
     3961 cccggtctct gtaccgggcc tttcgctaag ggaagatgta tgtcgttact tgcacaactg
     4021 gatcaaaaaa tcgctgctaa cggtggcctg attgtctcct gccagccggt tccggacagc
     4081 ccgctcgata aacccgaaat cgtcgctgcc atggcattag cggcagaaca ggcgggcgcg
     4141 gttgccattc gcattgaagg tgtggcaaat ctgcaagcca cgcgtgcggt ggtgagcgtg
     4201 ccgattattg gaattgtgaa acgcgatctg gaggattctc cggtacgcat cacggcctat
     4261 attgaagatg ttgatgcgct ggcgcaggcg ggcgcggaca ttatcgccat tgacggcacc
     4321 gaccgcccgc gtccggtgcc tgttgaaacg ctgctggcac gtattcacca tcacggttta
     4381 ctggcgatga ccgactgctc aacgccggaa gacggcctgg catgccaaaa gctgggagcc
     4441 gaaattattg gcactacgct ttctggctat accacgcctg aaacgccaga agagccggat
     4501 ctggcgctgg tgaaaacgtt gagcgacgcc ggatgtcggg tgattgccga agggcgttac
     4561 aacacgcctg ctcaggcggc agatgcaatg cgccacggcg cgtgggcggt gacggtcggt
     4621 tctgccatta cgcgtcttga acacatttgt cagtggtaca acacagcgat gaaaaaggcg
     4681 gtgctatgac cacgttggcg attgatatcg gcggtactaa acttgccgcc gcgctgattg
     4741 gcgctgacgg gcagatccgc gatcgccgag aacttccgac accagccagc cagactccgc
     4801 aggcgttgcg tgatgcctta gccgcgttag tcgccccgtt gcaagctcat gcgcagcagg
     4861 ttgccatcgc ttcaaccgga attattcgcg acggcagctt gctggcgctg aatccgcata
     4921 atcttggtgg attgctacac tttccgttag tcaaaacgct ggaacaactt tccaatttgc
     4981 cgaccattgc cattaacgac gcgcaggccg cagcatgggc agagtatcag gcgctggaag
     5041 gcgatataac cgatatggtg tttatcaccg tttccactgg cgtcggcggc ggtgtagtga
     5101 gcggcggtaa actgcttacc ggccctggcg gtctggcggg gcacatcggg catacgcttg
     5161 ccgatccaca cggcccagtc tgcggctgtg gacgcacagg ttgcgtggaa gcgattgctt
     5221 ctggtcgcgg tattgcagcg gcagcgcaag gtgaactcat gggcgcggat gcgagaacta
     5281 ttttcacacg cgccgggcag ggcgacgagc aggcgcagca actgattcac cgctccgcac
     5341 atgtgctggc aaggctgatc gctgatatta aagccacaac tgattgccag tgcgtggtgg
     5401 tcggtggcag cgttggtctg gcagaagggt atctggcgtt ggtggaaacg tatctggcgc
     5461 aggagccagc ggcatttcat gttgatttac tggcggcgca ctaccgccac gatgcaggtt
     5521 tacttggggc tgcgctgttg gcccagggag aaaaattatg atgatgggtg aagtacagtc
     5581 attaccgtct gctgggttac atcctgcgtt acaggacgcg ttaacgctgg cattagctgc
     5641 cagaccgcaa gaaaaagcgc cgggtcgtta cgaattacag ggcgacaata tctttatgaa
     5701 tgtcatgacg tttaacactc aatcgcccgt cgagaaaaaa gcggaattgc acgagcaata
     5761 cattgatatc cagctgttat taaacggtga ggaacggatt ctgtttggca tggcaggcac
     5821 tgcgcgccag tgtgaagagt tccaccatga ggatgattat cagctttgca gcaccattga
     5881 taacgagcaa gccatcatct taaaaccggg aatgttcgcc gtgtttatgc caagtgaacc
     5941 gcataaacca ggatgcgttg tcggcgagcc gggtgagatt aaaaaggtgg tggtgaaggt
     6001 taaggctgat ttaatggctt aaatttatta gatcaggtgt gacctgaccc atctttggca
     6061 cagagccaaa cctgaactga cggaccgctc tgtgccatta cctgacgttg tattctttta
     6121 tgttaacacc cttgatactg tatatacctg tccgcttaat tcgcttgccc tttttattcg
     6181 taatagttgc tgtaagtgta ctgcagcgaa tggaatgacg ttcatcaatc accagccgaa
     6241 ttgcttctga agcatcttcc acctctagaa ggtcgtgagt cttatttaag ccgaatggaa
     6301 caagatggaa ggcagttttt cctgatgatt ttctggttat ttttactgga tataacacat
     6361 caccactttt atggattaat tcaagctgtt caatgtaaat catggcaact ttccttcgtt
     6421 tatttgcatg aatatacaaa ccgaaaatag gagatgcaat acaaaagctg tggaaatatc
     6481 aaacagcctc tcaatttggc agacagcgtc tgccaaatct ttgccgatgg gaattctgca
     6541 gatggtgtct gcggaattcc caatggtgtt aattagccag acactgtctg gcgaatttca
     6601 acatgttgaa aaaagaggta aaaaagcctc atttggcgta agttttttgc agagaaccca
     6661 cgcttatacc ttagacttaa atcctttgat agtcgcttga t
//
LOCUS       DAJTNA010000196         6696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTNA010000196 DAJTNA010000000
VERSION     DAJTNA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.198"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..3246
                     /locus_tag="OO161_003528"
     CDS             <1..3246
                     /locus_tag="OO161_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5464460.1"
                     /translation="PPEGFESSGSETGLGVEVTKAKPVTRTITSANIDRLRVTFGVQS
                     LVQTTSKGDRNPSSVRILIQLQRNGRWVTEKDVTINGKTTSQFLASVILDNLPPRPFN
                     IRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNY
                     HIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAA
                     DVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRKAWDVLSDFCSAMRCMPVW
                     NGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGW
                     QTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLWVIKTGLLETQTVDFTLGS
                     QGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLDREVTLPETGTSAVNLING
                     SGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTF
                     AITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSTADGQYLAQAKWDT
                     PRVVKGGRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQG
                     EPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSEAKIADTSQVE
                     TSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLF
                     REKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEITQTVSKTLEDQSATIQQI
                     QRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMIN
                     PEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNADISG
                     NVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTVTV
                     RIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIY
                     SGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGISIS"
     gene            3314..3913
                     /locus_tag="OO161_003529"
     CDS             3314..3913
                     /locus_tag="OO161_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5464461.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            3978..5537
                     /locus_tag="OO161_003530"
     CDS             3978..5537
                     /locus_tag="OO161_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5464462.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVVRNAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGDASESAR
                     QAAESAASAKQSEEASSSSASAAAQKASESLQSATDAELSKKTAESAAGNAVRDATTA
                     TEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGD
                     TGPAGATGERGPAGDAGPAGPQGPKGDRGETGLTGNAGPQGAKGDTGAAGPAGAQGPK
                     GDKGDPGVAGPAGPAGAPGPKGDKGDPGVAGPAGPAGPQGPKGDIGAPGQGTELLTTA
                     NTWTQAQTFNGGINGNLTVTGNGSFNDIQIRSDKRNKRNLVKLDNALDRLEALTGYLY
                     EIQYSADGWQTSVGLIAQDAQKALPELVTEDADVISGEKRLRLNYNGIIALLVEGFKT
                     LRHEIKELREK"
     gene            5623..6135
                     /locus_tag="OO161_003531"
     CDS             5623..6135
                     /locus_tag="OO161_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464463.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            6211..6423
                     /locus_tag="OO161_003532"
     CDS             6211..6423
                     /locus_tag="OO161_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5464464.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
BASE COUNT         1659 a         1609 c         2047 g         1381 t
ORIGIN      
        1 ccaccggaag gctttgagtc ctccggctct gaaaccggac tgggcgtgga agtgacgaag
       61 gcaaaaccgg tgacgcgcac cattacgtcc gcgaacattg accgcctgcg ggttaccttc
      121 ggggtgcagt cactggtgca gaccacgtca aagggcgacc gtaatccttc ctctgtccgg
      181 attctgattc agttacagcg taatggccgc tgggtgacgg aaaaggacgt caccattaac
      241 ggcaagacca cctcacagtt cctggcctcg gtgattctgg ataatctgcc tccccggccc
      301 tttaacatcc ggatggtcag ggagacggcg gacagcacca cggaccagct gcagaataag
      361 acgctgtggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcc
      421 attgtggggc tgcaggtgga tgcggagcag ttcggcggcc agcagatgac ggtgaactac
      481 catatccgcg gtcgcatcat ccaggtgccg tcaaactatg acccggaaaa acgcacgtac
      541 agtggtatct gggacggcag tctgaaaccg gcatacagca acaacccggc ctggtgcctg
      601 tgggacatgc tgactcaccc gcgctacggc atgggaaaac gtctgggggc ggcggatgtg
      661 gacaagtggg cgctgtatgc catcgggcag tactgcgacc agacggtccc ggatggtttc
      721 ggggggaccg agccgcggat gacctttaat gcgtacctgg cacaacagcg taaggcgtgg
      781 gacgttctca gtgatttctg ctctgcgatg cgctgtatgc cggtatggaa cggtcagacg
      841 ctgacgttcg ttcaggaccg cccgtcggat gtggtgtggc cgtacaccaa cagcgatgtg
      901 gtggtggatg ataacggcgt gggattccgc tacagcttca gtgccctgaa ggaccggcac
      961 acggcggtgg aggtgaatta caccgacccg cagaacggct ggcagacctc cacggaactg
     1021 gtggaagacc cggaagccat actgcgctac ggacgcaacc tgctgaagat ggatgcgttt
     1081 ggctgtacct gccgcggtca ggctcaccgt gcaggtctgt gggtgataaa gaccggactg
     1141 ctggaaacgc agacggtgga cttcacgctc gggtcacagg ggctgcgtca cacaccgggt
     1201 gacattattg aaatctgtga taacgactat gccgggacta tgaccggcgg acgtgtcctg
     1261 tccattgatg ctgccacccg caccctgacg ctggaccgtg aggttaccct gccggagaca
     1321 ggtacatcgg cggtgaacct gattaacggc agcggtaagc cggtgagtgt ggacatcacc
     1381 gcacaccccg cgccggaccg gatacaggtc agtaccctgc ctgatggtgt ggagacatac
     1441 ggggtgtggg gactctccct gccgtcactg cgccgtcgcc tgttccgctg tgtctccgtc
     1501 cgggaaaaca cggacggcac ctttgccatc acggcggtgc agcacgtacc ggaaaaagaa
     1561 gccatcgtgg ataacggtgc ccgctttgag ccgcagtcag gctccctgaa cagcgtcatc
     1621 ccaccggcag tgcagcacct gacggtggag gtgagcacag ctgacggcca gtatctggca
     1681 caggcgaaat gggacacgcc gcgggtggtg aagggggggc gcttcagtct gcgactgacc
     1741 agcggaagcg gagaagacag ccgtctggtg accaccgcta tcactgcgga tacagagcat
     1801 cgtttcagtg gtctgccgct cggggaatac accctgacag tcagggcaat taacagttat
     1861 ggccagcagg gcgaaccggc caccaccacc ttccggatta acgcgccagc aaaacccgcc
     1921 accattgaac tgacgccggg gtattttcag ataacagcgg taccgcgtct tgcggtgtat
     1981 gacccgacgg tacagtttga gttctggttt tcggaggcaa aaatcgcaga cacatctcag
     2041 gtggaaacct ctgcccgtta tctggggacc ggcagtcagt ggagtgtatc cggcccgcac
     2101 attaagcccg ggaaggattt ctggttttac gtgcgcagcg tcaacctggt ggggaaatct
     2161 gcttttgtgg aggccagtgg acgggccagc aatgatgcag aagggtatct ggggctgttt
     2221 cgggaaaaaa taggaaaact gcatctggct caggggctgt gggagctgat agacaacagc
     2281 cagcttgcgg atgagatggc ggagatgaag accaccatca ccgaaacccg caatgaaatc
     2341 acacagacgg tcagtaaaac gctggaggac cagagcgcca ccatacagca gatacagcgc
     2401 gtgcagaagg acacaaatga tgaccttgct gcactttaca tgctgaaggt acagaaaaca
     2461 aaaaatggca taccctatgt tgccggtatt ggagcgggga ttgaggatac tgatggccag
     2521 cccctgagca acatactgct gctggctgac cgtattgcga tgattaaccc ggaggacggc
     2581 aacaccacgc cgttatttgt ggcgcagggg aatcagttgt tcatgaacga tgtgttcctg
     2641 aagcggctgt ttgcggtgag tatcacctcg tccggcaatc ccccgacgtt ttccctgacg
     2701 ccggagggca ggctgaccgc aagaaatgct gatatcagcg gtaacgtgaa tgcgaactcc
     2761 gggacgctca acaacgtcac gattaacgag aactgccggg ttctgggaaa actgtccgcg
     2821 aaccagattg aaggcgatct cgttaaaaca gtgggtaagg cttttccgcg ggactcccgg
     2881 gcaccggagc ggtggccatc agggaccgtt accgtcagga tttatgacga tcagcctttt
     2941 gaccggcaaa ttgttattcc ggcggtggca ttcagcggcg ctaagcatga gagagagcat
     3001 actgatattt actcctcatg ccgtctgata gtgcggaaaa acggtgctga aatttataac
     3061 cgtaccgcgc tggataatac gctgatttac agtggcgtta ttgatatgcc tgctggtcac
     3121 ggtcacatga cgctggagtt ttcggtatca gcatggctgg tgaataactg gtatcccaca
     3181 gcaagtatca gcgatttgct ggttgtggtg atgaagaaag ccaccgcagg catcagtatc
     3241 agctgaattt tataacccat atacgggcgc cagaaatggc gcctttttta ttgcagaaaa
     3301 gcgagaggta attatgcgta aagtttgtgc agccattttg tccgcagcca tctgtctgtc
     3361 cgtatccggt gcgcctgcat gggcgtctga acatcagtcc acactgagcg cgggatatct
     3421 tcatgcccgt acgaacgctc ccggcagcga taatctgaac gggattaacg tgaaataccg
     3481 ttatgagttt acggacgcgc tggggctgat tacgtccttc agttatgcca atgctgaaga
     3541 tgagaaaaaa acgcactaca gcgatacccg ctggcatgaa gattccgttc gtaaccgctg
     3601 gttcagcgta atggcggggc cgtctgtgcg cgtgaatgaa tggttcagcg cgtatgcgat
     3661 ggcgggtgtg gcttacagcc gtgtgtcgac tttctccggg gattatctcc gcgtaactga
     3721 caacaagggg aaaacgcacg acgtgctgac cggaagtgat gacggtcgcc acagcaacac
     3781 gtctctggcg tggggggctg gcgtgcagtt taacccgacc gaatccgtgg ccattgatat
     3841 tgcttatgaa ggttccggca gtggcgactg gcgcactgac ggtttcatcg tgggtgtcgg
     3901 ctataaattc tgattagcca ggtaacacag tgtcatgaca gcccgccggt tcaggcgggc
     3961 ttttttgtgg ggtgaatatg acagtaaaga tttctggtgt actgaaagac ggcacaggaa
     4021 aaccggtaca gaactgcaca atccagctga aagcaaaacg taacagtacc acggtggtgg
     4081 tgaacacgct ggcctcagaa aatccggatg aagccgggcg ttacagtatg gacgttgagt
     4141 atggtcagta cagcgttatt ctgttggtgg agggattccc gccgtcacat gccgggacta
     4201 tcaccgtgta tgaagattct caacccggta cgctgaatga ttttctcggt gccatgactg
     4261 aggatgatgc ccgtccggag gcactgcgtc gctttgaact gatggtggaa gaggtggtgc
     4321 gtaacgcaga ggaggcgaag aagaatgccg gagaggcgga gacgtcagcg aggaatgccg
     4381 gcatatcagc cagtcaggca gaagagagcg ctgcaaatgc tgacacttca gcaggggatg
     4441 catcggagtc agcccggcag gcggcagaaa gtgcagcctc agcaaagcag tcagaggagg
     4501 cgtcctcgtc ctcggcctct gcggccgctc aaaaagccag tgagtcatta caaagtgcaa
     4561 cagatgctga gttgtcaaaa aagacggcag aaagtgcagc cggtaatgca gtcagggatg
     4621 caacgaccgc aacagaaaaa gcccgggagt cagcagaaag cgcacagtca gcggaacaaa
     4681 gcaggatagc ggcggaagag gccgtaaacc gaatccccac cgtggtggga cctcccgggc
     4741 caaaggggga accggggccc gcgggtcctc aggggccgaa gggagataaa ggagagcgtg
     4801 gcgacaccgg cccggcaggg gcaaccggtg aacgggggcc ggcaggtgat gctggtccgg
     4861 caggcccgca ggggccgaaa ggcgacaggg gagagaccgg tctgacggga aatgcaggtc
     4921 cacagggggc aaagggagat acgggagctg caggcccggc aggcgcacag gggccaaaag
     4981 gtgacaaagg cgatccggga gtggctggac cagcaggtcc ggcaggtgcg ccggggccga
     5041 aaggcgataa aggtgatccg ggagtagcag gtccagcagg tccggcgggg ccacagggac
     5101 cgaagggaga cataggagcc cccgggcaag gaacagaact gcttactact gccaatacat
     5161 ggactcaggc acaaactttt aatggtggta ttaatggcaa tttgacggtg accggaaacg
     5221 gctcatttaa cgatattcag atccgttcgg ataaacgcaa caagcgaaat ctggtaaaac
     5281 tggataatgc gttagatcgt ctggaggcac ttactggtta tctttacgag atacagtact
     5341 ctgccgacgg ttggcaaacg tcggttggtt taattgctca ggatgcacaa aaagcattgc
     5401 ctgaactggt aactgaagac gcagacgtta tatctggtga aaaacgtctg cgtcttaact
     5461 acaacggcat aattgcattg ttagtcgagg gctttaaaac acttcgtcat gagattaaag
     5521 aactccggga gaagtaaacg acagctgttg tagtttctgg tttctactga atttaaattg
     5581 tggggtgaca ctcaccccac gcattcagaa gggggagatg agatgggggt aacatcggga
     5641 tgggtaggct cttcggctaa gagcgaaaca ggtgagcagt ggatgggagc cgccggaacc
     5701 aaactgggac tgagtaaacc ttttatgatg agccaaatgg ttgggcgaac tatgggctgt
     5761 aaaattgcaa ctgagtacta taaatggaaa tcctctgaca aggttgataa ctggggcgca
     5821 gttggcgctg actggccttt agaagaaaaa agtaaaggta caattacaaa cgccgcaagc
     5881 tgcggatcag ggaggctggt aggggctgtc gttacacttt ctcacttttt gacgaactct
     5941 acaccgacag ctgctgttta tttagccggt ggtaaagcag gtaacatcac cgtaaacgta
     6001 ggtggtgcta cacaaaccat gatttatcag ggcgttgtta gtgggttcca gtattactgg
     6061 tcaggttctg ttagttccgc tttcgtggag gcaatgaaaa agacgggagt accccaggat
     6121 ctaaaaatta gttaaatggt aaataaataa ttttaaaaac ttcacgttct acataccgga
     6181 gacgccggat atataggata ttgttttaag ttgccagaga aatttttccg gacggatgct
     6241 gataataatg atgtgccaat gacggcagca tcatggatgg cactgagtga agcgacagag
     6301 caggcgatgt ttgcgaaggg agtggagatt aatacacggc agttgcagat gaaagccgag
     6361 gttgaggcgc tgacagacct gaaggcgatc cgcagttatg ttgtcggatg gcctgctggc
     6421 tgaataaaaa aacgggacca cgaccagtcc cggaaccatg agttttaggg tattagtttg
     6481 ttatcatagt tagcgtgcta agtatgccat gtcaggttga ttagtgaagt gatgttgttc
     6541 gcatttttgc acggcggaga atattcagat ttttggaaat ccatattttt cccgtgcgcg
     6601 attacatgct tcatttacga tgctattttc gccggacatt gcgaattccc tgcatgtgga
     6661 cggtttttgt aaacagagca atatgcgttt tctccg
//
LOCUS       DAJTNA010000197         6685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTNA010000197 DAJTNA010000000
VERSION     DAJTNA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.199"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            199..939
                     /locus_tag="OO161_003533"
     CDS             199..939
                     /locus_tag="OO161_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5464465.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(910..1677)
                     /locus_tag="OO161_003534"
     CDS             complement(910..1677)
                     /locus_tag="OO161_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5464466.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="OO161_003535"
     CDS             complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="OO161_003535"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5464467.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGHEGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2701..5145
                     /gene="fadE"
                     /locus_tag="OO161_003536"
     CDS             2701..5145
                     /gene="fadE"
                     /locus_tag="OO161_003536"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5464468.1"
                     /translation="MMILSILATVVLLGALFYLRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5188..5634)
                     /gene="ivy"
                     /locus_tag="OO161_003537"
     CDS             complement(5188..5634)
                     /gene="ivy"
                     /locus_tag="OO161_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5464469.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            5815..6585
                     /gene="yafV"
                     /locus_tag="OO161_003538"
     CDS             5815..6585
                     /gene="yafV"
                     /locus_tag="OO161_003538"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5464470.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT         1542 a         1704 c         1821 g         1618 t
ORIGIN      
        1 atcttaggaa ataaatgata catgcgcttt tcggactatt gaaaactgcc cctgtgctaa
       61 atttgtaaac cctgcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt
      121 ccaaatccag actattccga tttgagatcc attttgccgt cctggcgtgt gtaaccgttt
      181 tatcaaggaa taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg
      241 tgtgtttcgt ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag
      301 tataagcagc agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg
      361 ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta taaaatctgt
      421 aaatattccg gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg
      481 ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat
      541 attggtttcc ccaatgccta tgaccgtgcg catggttatg aagggaaata cctgatgatc
      601 cacggcgatt gtgtttccat cggctgctac gcaatgacca atcagggtat tgatgagatc
      661 ttccagttcg ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt gagtatttac
      721 ccgttccgca tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc
      781 tgggagcaac tgaagccggg ttacgactac tttgagcaaa cccgtaagcc accgaccgtt
      841 tctgtggtca atggtcgtta cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca
      901 caactggcat caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat
      961 cttttgccag gtttcattgc ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt
     1021 cggtgtggtc tgcgagctga agtcgatttc tacatcctga tccagcagcg ttgccacgcc
     1081 aaacggtgcg cggcgggtga tccagtgcag attcgtcgag caatacgcca ttacatagcg
     1141 cccatccgaa agcagcatgt tgaaaacgcc cttctgccgc agttcgtccg ccagtgaagc
     1201 gatatattta aacactgctg ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt
     1261 atgcagcagc cagcagaagg ctttttcgct gtcggtttcg cctaccgggc ggaagttgcc
     1321 ggtttccagt gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc
     1381 ccataactcg cgggtaaatg ggtgagtatt ttccaacgcc acctcgcccc gattagcctg
     1441 gcgaatatga gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat
     1501 gggggaatta aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa
     1561 ggtaatgccc cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt
     1621 gaaactaaag cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg
     1681 taacctccac aacagcggga gcgcatggcc cccggcgaag attgatttaa gaattttcag
     1741 gtcggatgcg gcgtaaacga attattcggc ctacggttcg cgtacagatt tgtaggcatg
     1801 ataagacgcg tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg
     1861 ccttatccgg cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga
     1921 tatggatcac tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc
     1981 gaatttcgat atccgccgtg ccagccattt tgccgccgtc tttaccggtc agggtgatca
     2041 ctttcatccc cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg
     2101 aggtggagat ccccagcagt acatcgcctt cgtgacctac cgcttcaacg tagcgggaga
     2161 aaatatcatt gaaaccgaaa tcattaccga cgcaggaaat atgactaacg tcagaaatag
     2221 caatcgccgg gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt
     2281 gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa
     2341 agctgtctgc taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta
     2401 aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcctggtaca
     2461 tgaggatatc cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa
     2521 agcgagaagt acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa
     2581 cagcaacaat gtgagctttg ttgtaattat attgtaaaca cattgctaaa tgtatttaca
     2641 tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt
     2701 atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatctccgc
     2761 gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct
     2821 ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt
     2881 gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gaggtttccg taaggtgatg
     2941 ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc
     3001 gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg
     3061 accgccgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat
     3121 gatttccaga ttactcatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa
     3181 gagcatcgct tcttcgcgat gattatcaaa aaagagtacg gcggactgga gttctcagct
     3241 tatgcccagt ctcgcgtgct gcaaaaactc tccggcgtaa gcgggatcct ggcgattacc
     3301 gtcggcgtgc cgaactcatt aggcccgggc gagctgctgc aacattacgg cactgacgag
     3361 cagaaagatc actatctgcc gcgtctggcg cgtggtcagg agatcccttg ctttgcactg
     3421 acaagcccgg aagcgggttc cgatgcgggc gcgattccgg acaccgggat tgtctgcatg
     3481 ggcgaatggc agggccagca ggtgctgggg atgcgtctga cctggaacaa acgctatatt
     3541 acgctggcac cgattgcgac tgtgcttggg ctggcgttta aactctccga cccggaaaag
     3601 ctgctgggtg gcgcagaaga tttaggtatt acctgtgcgc tgatcccgac caccacgccg
     3661 ggcgtggaaa ttggtcgtcg ccacttcccg ctgaacgtac cgttccagaa cggaccgacg
     3721 cgcggtaaag atgtcttcgt accgatcgat tacatcatcg gtgggccgaa aatggccggg
     3781 caaggctggc ggatgctggt ggagtgcctc tcggtaggcc gcggcatcac cctgccttcc
     3841 aactcaaccg gcggcgtgaa atcggtagcg ctggcaacgg gcgcgtatgc tcacattcgc
     3901 cgtcagttca aaatctctat cggtaagatg gaagggattg aagagccgct ggcgcgtatt
     3961 gccggtaatg cctacgtgat ggatgctgcg gcatcgctga ttacctacgg cattatgctc
     4021 ggcgaaaaac ctgccgtgct gtcggctatc gttaagtatc actgtaccca ccgcgggcag
     4081 cagtcgatta ttgatgcgat ggatatcact ggtggcaaag gcattatgct cgggcaaagc
     4141 aacttcctgg cgcgtgctta ccagggcgca ccgattgcca tcaccgttga aggggctaac
     4201 attctgaccc gcagcatgat gatcttcgga caaggagcga ttcgttgcca tccgtacgtg
     4261 ctggaagaga tggaagcggc gaagaacaat gacgtcaacg cgttcgataa actgttgttc
     4321 aaacatatcg gtcacgtcgg tagcaacaaa gttcgcagct tctggctggg cctgacgcgc
     4381 ggtttaacca gcagcacgcc aaccggcgat gccactaaac gctactatca gcacctgaac
     4441 cgcctgagcg ccaacctcgc cctgctttct gatgtctcga tggcagtgct gggcggcagc
     4501 ctgaaacgtc gcgagcgcat ctcggcccgt ctgggggata ttttaagcca gctctacctc
     4561 gcctctgccg tgctgaagcg ttatgacgac gaaggccgta atgaagccga cctgccgctg
     4621 gtgcactggg gcgtacaaga tgcgctgtat caggctgaac aggcgatgga tgatttactg
     4681 caaaacttcc cgaaccgcgt ggttgccggg ctgctgaatg tggtgatctt cccgaccgga
     4741 cgtcattatc tggcaccttc tgacaagctg gatcataaag tggcgaagat tttacaagtg
     4801 ccgaacgcca cccgttcccg cattggtcgc ggtcagtacc tgacgccgag cgagcataat
     4861 ccggttggct tgctggaaga ggcgctggtg gatgtgattg ccgccgaccc aattcatcag
     4921 cggatctgta aagagctggg taaaaacctg ccgtttaccc gtctggatga actggcgcac
     4981 aacgcgctgg cgaaggggct gattgataaa gatgaagccg ctattctggt gaaagctgaa
     5041 gaaagccgtc tgcgcagtat taacgttgat gactttgatc cggaagagct ggcgacgaag
     5101 ccggtaaagt tgccggagaa agtgcggaaa gttgaagccg cgtaattgaa taacggagcc
     5161 gaaaggctcc gtttctttat ccgctaatta tttaaaatta aagccatccg gatggttttc
     5221 caggctgccg gtcaacgccg cgaacagcac cgttttacca tcaatcgaaa gcgcatcgtt
     5281 cacattcagc caggtgagtt tctcttgcga cgttttctca tcaatagtcg agaacagccc
     5341 cgtcatctga ttagatttct cggaccacat cacagcgata cgttgcgagc cacagtcatg
     5401 cggtttgcac gcgctcatca cctgatacgt ctcatctccc aacgttacgg tttgtgcggg
     5461 agtataagta ccgcctttca tcacccaggc aggcagctta tgcccttgta ccatctgatt
     5521 aaatgcagct ttggtggttt cgccctttgc aaggctgcta atggttaaat catcctgcgc
     5581 cattgcactg gtggcgatga ccagagcggc gactgtcgtt attgccttaa acatcattcc
     5641 tcccgagctt atcctgccca tgttattaac cttctaaagc ctggcgataa cacgataatt
     5701 tgcaaatcag atatttccaa tatcagcatt tgttatcaat ttattctgcg ctaaggcaac
     5761 tgcctgataa ggtgttaaag ttataaaacc cttcgcataa aggagttgta caccgtgcct
     5821 ggtttgaaga ttacgctttt gcagcaacca ctggtgtgga tggatggccc tgccaacctg
     5881 cgtcattttg atcgtcaact ggaaggtatt accgggcgcg atgtgatcgt tctaccggag
     5941 atgtttacca gcggctttgc catggaagcg gcagcttcgt cgctagcaca agatgacgta
     6001 gtgaactgga tgacagctaa ggcgcagcag tgcaatgcgc tgattgcagg cagtgttgca
     6061 ttacaaacgg agtctggttc ggttaaccgc tttttgctgg ttgagccggg cggcacggta
     6121 catttttatg ataagcgtca tctgttccgc atggcagatg agcatctaca ttataaagcg
     6181 ggcaatgcgc gagtgattgt ggaatggcgc ggctggcgta ttttgccgtt ggtgtgctac
     6241 gacttacgtt ttccggtgtg gtcgcgcaat ctcaacgatt atgacctcgc catctacgtt
     6301 gccaactggc ctgctccgcg ctctctgcac tggcaggcat tgctgacggc ccgcgcgatt
     6361 gagaatcagg cgtatgtggc gggatgcaat cgcgtcggca gcgatggcaa cggctgccat
     6421 tatcgcggtg acagccgggt gattaatcca caaggcgaga ttatcgctac tgccgacgcg
     6481 catcaggcaa cgcgcattga tgcggagctg tcgatgatgg cattgcggga atatagagaa
     6541 aagtttccgg catggcagga tgcggatgag tttaggttgc ggtgagtggg ggttgtgttt
     6601 aaggctgggg gagagtcggg gtaagactac gaaagcccgc tctccgcaag gactgacgcc
     6661 agatagtttc tgtccatagc tgctt
//
LOCUS       DAJTNA010000198         6581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTNA010000198 DAJTNA010000000
VERSION     DAJTNA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.201"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..175
                     /gene="tnaA"
                     /locus_tag="OO161_003539"
     CDS             <1..175
                     /gene="tnaA"
                     /locus_tag="OO161_003539"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="tryptophan indole-lyase; catalyzes the formation of
                     indole and pyruvate from tryptophan; also has xysteine
                     desulfhydrase activity; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5464471.1"
                     /translation="AELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEP
                     KVLRHFTAKLKEV"
     gene            266..1512
                     /gene="tnaB"
                     /locus_tag="OO161_003540"
                     /pseudo
     CDS             266..1512
                     /gene="tnaB"
                     /locus_tag="OO161_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
     gene            1644..2819
                     /gene="mdtL"
                     /locus_tag="OO161_003541"
     CDS             1644..2819
                     /gene="mdtL"
                     /locus_tag="OO161_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5464472.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSITVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            2794..3753
                     /gene="yidZ"
                     /locus_tag="OO161_003542"
     CDS             2794..3753
                     /gene="yidZ"
                     /locus_tag="OO161_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5464473.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            3910..4659
                     /locus_tag="OO161_003543"
     CDS             3910..4659
                     /locus_tag="OO161_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5464474.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            4681..5247
                     /gene="yieF"
                     /locus_tag="OO161_003544"
                     /gene_synonym="chrR"
     CDS             4681..5247
                     /gene="yieF"
                     /locus_tag="OO161_003544"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5464475.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5301..6581)
                     /gene="adeP"
                     /locus_tag="OO161_003545"
     CDS             complement(5301..6581)
                     /gene="adeP"
                     /locus_tag="OO161_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5464476.1"
                     /translation="MLERVFKLREHGTTARTEVIAGFTTFLTMVYIVFVNPQILGVAG
                     MDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVVVQAMGLPWQIGMG
                     AIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMGLKNAGVIVANSET
                     LVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLGWMLGDVHYNGIVS
                     APPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVTDKAGLADEKGKFP
                     RMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAVVVGLLFLLVIFLS
                     PLAGMVPGYAAAGALMTSSLARVNWQDLTESVPAFITAVMMPFSFSITEGIALGFISY
                     CVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1582 a         1688 c         1632 g         1679 t
ORIGIN      
        1 ggctgaactg ctgcgtttaa ccattccgcg cgcaacatat actcaaacac atatggactt
       61 cattattgaa gcctttaaac atgtgaaaga gaacgcggcg aatattaaag gattaacctt
      121 tacctacgaa ccaaaagtat tgcgtcactt caccgcaaaa ctgaaagaag tttaattaat
      181 actacagagt ggctataagg atgttagcca ctctcttacc ctacatcctc aataacaaaa
      241 atagccttcc tctaaaggtg gcatcatgac tgatcaagct gaaaaaagca ctctgcattt
      301 tggggtgtta tggttatagc aggtacagta attggtggag gtatgtttgc tttacctgtt
      361 gatcttgccg gtgcctggtt tttctggggt gcctttatcc ttatcattgc ctggttttca
      421 atgcttcatt ccgggttatt gttattagaa gcaaatttaa attatcctgt cggctccagt
      481 tttaacacca tcaccaaaga tttaatcggt aacacctgga acattatcag cggtattacc
      541 gttgccttcg ttctctatat cctcacttat gcctatatct ctgctaatgg tgcgattatt
      601 agtgaaacga tatcaatgaa tttgggctat cacgctaatc cacgtattgt cgggatctgc
      661 acagccattt tcgttgccag cgtattgtgg ataagttcgt tagccgccag tcgcattacc
      721 tcattgttcc tcgggctgaa gattatctcc tttgtgatcg tgtttggttc tttcttcttc
      781 caggtcgatt actccatcct gcgcgatgcc accagcacca ctgcgggaac gtcttacttc
      841 ccgtatatct ttatggcttt gccggtgtgt ctggcgtcat ttggtttcca cggcaatatt
      901 cccagcctga ttatttgcta tggaaaacgc aaagataagt taatcaaaag cgtggtattt
      961 ggttcgctgc tggcgctggt gatttatctc ttctggctct attgcacgat ggggaatatt
     1021 ccgcgcgaaa gctttaaggc gataatctcc tcaggcggca acgttgattc gctggtgaaa
     1081 tcgttcctcg gcaccaaaca gcacggcatt atcgagtttt gcctgctggt gttctctaac
     1141 ttagctgttg ccagttcgtt ctttggtgtc acgctggggt tgttcgatta tctggcggac
     1201 ctgtttaaga ttgataactc ccacggcggg cgtttcaaaa ccgtgctgtt aaccttcctg
     1261 ccacctgcgt tgttgtatct gatcttcccg aacggcttta tttacgggat cggcggtgcc
     1321 gggctgtgcg ccaccatctg ggcggtcatt attcccgcag tgcttgcaat caaagctcgc
     1381 aagaagtttc ccaatcagat gttcacggtc tggggcggca atcttattcc ggcgattgtc
     1441 attctctttg gtataaccgt gattttgtgc tggttcggca acgtctttaa cgtgttacct
     1501 aaatttggct aaatccttca agaagccagc cattcgctgg cttcttgcct ctcaggaaat
     1561 cacttatgtc caaatggcaa ctcgcctgat cctccttcac cacgtatgct ttgcgtcacc
     1621 ttactatcag gacgctttag cccatgtccc gctttttgat ttgtagtttt gccctggttt
     1681 tactttatcc cgccgggatt gatatgtacc tcgttggttt accgcgcatc gccgccgatc
     1741 tcaatgccag cgaagcgcag ttgcatattg cgttctccgt atatctggcg gggatggcag
     1801 ctgcgatgtt atttgccggt aaagtggccg atcgttcagg gagaaagccg gtcgccatac
     1861 ccggcgcggc gctatttatt attgcctcgg tgttctgttc actggctgaa accagcacgt
     1921 tatttcttgc aggccgattt ctacaggggt tgggcgcagg ctgttgttac gtagtggcgt
     1981 tcgccatttt gcgcgacacg ctggatgatc gacgtcgggc taaagtgctg tcattactca
     2041 acggtattac ctgcatcatt ccggtgttag cgccagtgct cggacatctg attatgctta
     2101 aattcccgtg gcagagtctg ttctgggcga tggcaatgat gggcatcgcg gtactgatgt
     2161 tgtctttgtt tattttaaaa gaaacgcgcc cagcgtcccc cgctgcttcg gacaaaccac
     2221 gagaaaatag cgagtcgctg cttaatcggt ttttcctcag ccgtgttgtt atcaccaccc
     2281 tcagcgtttc ggtgatcctc actttcgtca atacatcgcc ggtattgctg atggaaatca
     2341 tgggttttga gcgcggagaa tacgccacca ttatggcgtt gaccgctggc gtcagcataa
     2401 ccgtttcatt ctccacgcca tttgcgctgg gaatttttaa gccacgtacg ttgatgatca
     2461 cctcacaggt gttattcctt gcagcgggga tcacccttgc cgtttcacct tcccatgcgg
     2521 tttctctgtt tggtatcaca ctgatttgcg ccggtttctc ggtaggtttt ggcgtagcga
     2581 tgagtcaggc gttaggacca ttttcattac gcgcgggcgt agccagctcg accttaggta
     2641 ttgcgcaggt ttgcggttcg tcactgtgga tttggctggc agcggtggtt ggtatcggcg
     2701 catggaatat gctgatcggg attctgattg cctgtagcat agtgagcctg ttgctgatta
     2761 tgttcgtcgc gcctggacgc cccgttgccg ctcatgaaga aatccatcac cacgcttgat
     2821 ctcaatctgc tgctctgtct gcaactgctg atgcaggagc gcagcgtgac caaagcggcg
     2881 aagcggatga acgtgacccc ttcagcggtg agtaagtcgc tggcaaagtt aagagcgtgg
     2941 tttgacgacc cgctctttgt gaactcaccg ctggggctgt cgcccacacc gctaatggtc
     3001 agcatggagc aaaatctggc ggaatggatg caaatgagca atctgctgct ggataaaccg
     3061 caccaccaga caccgcgcgg cctgaagttt gagttggcgg cggaatcgcc gctgatgatg
     3121 atcatgctta atgcgctgtc gaaacggatc taccaacgtt acccgcaggc gaccatcaaa
     3181 ctacgtaact gggattacga ttccttagat gccattactc gtggtgaagt ggatattggt
     3241 ttttccggtc gcgaaagcca tcctcgctcg cgggagctgt taagctcgct accgttagcc
     3301 attgattatg aagtgctgtt tagtgatgta ccctgcgtct ggttacgcaa agatcatccg
     3361 gcactgcatg aaacgtggaa tctggacacc tttttacgtt atccgcatat cagcatttgc
     3421 tgggaacaga gcgacacctg ggcgctggac aatgtgttac aggagctcgg gcgcgaacgc
     3481 acgattgcta tgagcctgcc ggaatttgag cagtcactgt ttatggcagc gcaacccgac
     3541 aatctgctac tggcgaccgc gccgcgctac tgtcagtact acaatcaact ccatcaactg
     3601 ccgttggttg ctcttcctct cccgtttgac gaaagccagc aaaaaaagct ggaagttcct
     3661 tttaccctgc tgtggcataa acggaacagc cataatccga agatcgtctg gttacgggaa
     3721 accattaaaa acctttacgc gtcgatggca taaccgaatc gtatgaaacg cgacccaatt
     3781 tcacaataaa atgtaaaaaa gttgtaataa gcttgtctga atcgaacttt tagccgcttt
     3841 agtctgtcca tcattccagt aaatgattac tcttatattc ataatggacc attaagcatg
     3901 gagcgaaaaa tggcgactca ctttgcccga gggattttaa cggaaggaca tctgatttct
     3961 gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca ttccccctca tcgtcaaagc
     4021 cgttttctgg cgtccagagg tttactcgca gaactgatgt tcatgctgta tggcattggc
     4081 gaattgccgg aaatcgtcac cctgccgaaa ggtaaaccgg ttttcagtga taaaaatttg
     4141 ccttcgtttt ctatttccta tgccgggaat atggttggcg tggcgttaac aaccgaaggt
     4201 gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg ggtttcatag cccacacgcg
     4261 cccgataacc acaccttttc cagcaatgaa tcgctatgga tcagtaaaca aaacgatcct
     4321 aacgaagcgc gggcgcagct catcacgctg cgccgaagcg tgctaaaact aaccggtgat
     4381 gttttgaatg acgatccgcg cgatctgcag ctgctgccca ttgccggacg cctgaaatgt
     4441 gctcatgtaa atcatgtaga agcgttatgc gacgcggagg acgtgctggt gtggtccgtg
     4501 gcggtcacgc ccgcgattga aaagctcagt gtctgggagt tagatggcaa acacagctgg
     4561 aaaagcctgc cggatattca cagccgcgcc aacaatccta ccagccggat gatgcgtttt
     4621 gcccagctct ctaccgtgaa gtctttttcg ccaaattgat agacaaccac aggagtcatt
     4681 atgtctgaaa aattgcaggt ggttacgtta ctggggagcc tgcgcaaagg ctcatttaat
     4741 ggcatggttg cacgtaccct gccgaaaatt gctccggcga gcatggaagt caatgcgtta
     4801 ccttccattg ccgacattcc cctgtatgac gctgacgtac agcaggaaga aggttttcca
     4861 gcaacggttg aagctctggc ggaacagatc cgtcaggctg acggtgtggt gatcgtcacg
     4921 ccggaatata actactcggt accgggtggg ctgaaaaatg ccatcgactg gctttcccgc
     4981 ctgccggatc aaccgctggc cggtaaaccg gtgttgattc agaccagctc aatgggcgtg
     5041 attggcggcg cgcgctgtca gtatcacctg cgccagattc tggttttcct cgatgcaatg
     5101 gtgatgaaca agccggaatt tatgggcggc gtgattcaga acaaagttga tcggcaaacc
     5161 ggagaagtga ttgatcaggg tacgctggac cacctgaccg ggcaattgac cgcatttggt
     5221 gagtttattc agcgagttaa gatctaaata aaaaacccgc cagtaatcat gcatggcggg
     5281 tttttaatgc gctatcgatt ttaatgagcg tcgataaata caatcttcag gataaacagc
     5341 agcgcaacga tgattacgca cgggctaagg tcacgcagac gcccggtacc aatcttcatc
     5401 acgcagtagg agataaagcc cagcgcaata ccttcggtaa tcgaaaagct gaacggcatc
     5461 atcacggcgg taataaacgc cggaacagat tcagtaagat cctgccagtt cacgcgagca
     5521 agacttgagg tcatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     5581 gacagaaaga taaccagcag gaacagcaga ccaacaacca ctgccgtcag accggtacga
     5641 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     5701 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     5761 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     5821 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     5881 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     5941 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     6001 accgctgcgt gaatgttgcg cgaggccaga atagcaatga tgaagaagcc gaggataccc
     6061 agaagtacgc tgtgagaagt cagattaccg atgctcacca gcgtttccga gttagcgaca
     6121 atcacacctg cgtttttcag ccccatcatg ccgataaaca ggccgatacc gctggtaata
     6181 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     6241 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     6301 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     6361 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     6421 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     6481 aaaacgatgt aaaccatcgt caggaaggtg gtaaaaccgg cgatcacttc ggtccgtgcc
     6541 gtcgtgccat gttcgcgcag tttaaacacg cgttccagca t
//
LOCUS       DAJTNA010000199         6552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTNA010000199 DAJTNA010000000
VERSION     DAJTNA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.202"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..214)
                     /locus_tag="OO161_003546"
                     /pseudo
     CDS             complement(1..214)
                     /locus_tag="OO161_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            491..1366
                     /locus_tag="OO161_003547"
     CDS             491..1366
                     /locus_tag="OO161_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR5464477.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1374..2150
                     /locus_tag="OO161_003548"
     CDS             1374..2150
                     /locus_tag="OO161_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR5464478.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTFS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2319..4580
                     /locus_tag="OO161_003549"
     CDS             2319..4580
                     /locus_tag="OO161_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5464479.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4649..5824
                     /gene="senB"
                     /locus_tag="OO161_003550"
     CDS             4649..5824
                     /gene="senB"
                     /locus_tag="OO161_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR5464480.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6197..6385)
                     /locus_tag="OO161_003551"
                     /pseudo
     CDS             complement(6197..6385)
                     /locus_tag="OO161_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT         1817 a         1549 c         1528 g         1658 t
ORIGIN      
        1 ccgggatgtg ctgtttcagc ctcgcgacca gctcacgctg tgtcagcgtt tccgtcgccg
       61 tttctcccgt tttgtggtcc aggtaacgga agttcaggct cgcccctccg ttgtaatgtg
      121 ccagccggga ggccgctatc ggcggcttct tcagataacg acccaggtag cgcgccgtat
      181 tcctcccgcc ggccgttttc ttcgacatgt acatcaggcg cggaactcat cggggcgcgg
      241 gaatgaaatc ccattcagac tccggataga ttcaagcaag cccacttatc gtcaaaatcg
      301 gtgttgcaaa aacgggagtg accatagatc cgttttccaa gcggacccca taatatcggc
      361 tggtaaacat cactcgctag tgcattcttt ttgttggtaa tgataattat ttacatttat
      421 tctttgatgt tttatcataa ttaatgtttt atcaataaat atatggaaat atattgtttc
      481 agatgggaat atgagaaagt tcatccttat ttctatgata acgttagcta gtgttagtct
      541 gggagcctgc agacagaatg tcaccataaa gcaggatgcg ccagggcaga aagctttcct
      601 gattgacggg cagatttacg atttgcacag tggcaaaatt atttctagca gcgagctttt
      661 ggcggatctt gctaccgctc gacatcttat tattggtgaa aaacacgata atgccgagca
      721 tcatcagatt gagctttggc ttattcaaaa tctgctcatc cagcgaccac aggggagtgt
      781 gttgcttgag atgttgacgt cagaccaaca gccccgagtt aaccaagtta aatgttggct
      841 gaaagataat cctgtggttc gagatagccg tgttcaggaa ctacttaact ggcaaaaagg
      901 gtggtcctgg gagatgtatg gcgacatcgt catgcagcta ttacgtgggc cttacccgct
      961 gctcaatgcc aacattggcc gggagcagat gttggcactg tacaaaaaaa acgaatttcc
     1021 taaaggaaaa aaatccactg cacctgtggt acaagaagct ctgcgtgaaa ccatcatagc
     1081 tatgcatgaa ggcaaccttg aaagccagca actgacgtca atgctctctg ttcagcagca
     1141 gcgggatcgc tacatggccc gccagcttct gagtgccccg gtaccgtcgt tgttgattgc
     1201 aggtggttat cacgcgtcaa aaagcatggg cgtaccgtta cacatggaag atcttgccac
     1261 cggcacccat ccagtggtgt tgatgcttgc agaaaaagga atgaacatca cagtagatca
     1321 tgctgactac gtgtggtttg tagcgccaga tacaacaaaa aggtaaacca tggatgatga
     1381 atattctcca cttcccacag tcggtaaaat ggagcagctg gtttatctgt tctctactgc
     1441 ttcatggact gattttcctt acgtttatct ggcgtttttc cgaagttcag cccgctatgt
     1501 ctcctgcacc ggcgatcatg ctgcaatggg cagaaattat agaggctcct tcttctccac
     1561 tttcgcttcc ggtgggaatc gctcaacagg agtccgcagt gacagaagaa aagcaacaaa
     1621 ctgaggacag acaacaacgt cctgtcactg aagacagtga tgcaaccatt gaaataacta
     1681 ggaagaaaaa gtcatccgat ggagaaaaga aaaagacacg tccacctcgt aagataaagg
     1741 ctcaaaccag cgacagcaac cccacagccg tttccagtaa tgctgcacct caggctctgg
     1801 tcgaatcgtc ccgtattgct gcgcctttta acagtgactc aacaaagcga gataattctg
     1861 aggcatcttg ggaaagcaga gtaaaaggcc accttaaccg ttataaacgt taccctggcg
     1921 atgccagaaa acgtgcacga accggcacag ccgttgtgac ttttaccgta aacacggaag
     1981 gcactattgt cagtagtttc ttggaaatct cttcaggaac cttttctctt gaccgcgaag
     2041 ctattgctgt tttagaacgc gctcaaccac ttcccaaacc accgcctgaa atattggagg
     2101 gaggattatt taaagtaaaa atgccgatta cctttaagtt gaaggagtaa agggataatt
     2161 ggccgttcaa gttcatttaa ctgaaattta attttcattt ttatgctgaa cattttatca
     2221 aacttttcca ctgtcgaaaa aataaaaaag acagtagatc ataattaata aacaaatagt
     2281 tatacttatc tttattaata ttatatccaa ggaaatttat gaacgttata aaactggcta
     2341 tcggctcagg catattattg ctcagctgcg gtgcttactc acaatccatc agtgaaaaaa
     2401 ctaattccga caaaaaagga gcggcagaat tcagtccgct cagcgtttct gtcgggaaga
     2461 cgaccagtga gcaggaagct ctcgagaaaa caggcgcgac cagttcccgg acaacggaca
     2521 aaaacctgca atcacttgac gcaacagtgc gtagtatgcc tggtacttat actcaaatag
     2581 atcctggtca gggagcaatc agtgtgaata ttcgaggcat gagcggattt ggtcgtgtaa
     2641 acactatggt cgatggtatt acccagagtt tttacggaac ctctacctcc ggaacaacgg
     2701 cgcatggttc aactaacaat atggctggcg tacttataga tcctaactta ctggtagcag
     2761 ttgatgttac acgcggtgac agcagtggct ctgaagggat caacgccctt gccggtagtg
     2821 caaatatgcg tactattggc gttgacgatg taatatttaa cggtaataca tatggccttc
     2881 gttcacgttt ctctgtcggt agtaatgggc tgggacgcag cggaatgatc gcccttggtg
     2941 gaaaaagcga cgcttttacg gatacgggaa gcattggcgt tatggctgct gtgagcggca
     3001 gttctgtgta ctctaatttc tcaaatggtt ctggaattaa cagcaaagag tttggttatg
     3061 ataaatatat gaagcagaac cccaaatccc aactgtataa aatggatatc agaccagacg
     3121 aatttaacag cttcgaactt tccgctcgaa cctatgaaaa taaatttaca cgtcgtgata
     3181 taaccagtga cgactattac attaaatatc attacacccc tttttctgaa ttaattgact
     3241 ttaacgtaac ggccagtacc agtcgcggta atcaaaagta tcgtgatggc tcgctgtata
     3301 ctttctacaa aacctcagcg caaaatcgtt ctgacgcgct ggatatcaac aataccagcc
     3361 ggttcactgt cgcggacaat gaactggagt ttatgctggg cagcaaactg atgcgtaccc
     3421 gctatgaccg gaccattcac tcagcggcgg gcgacccgaa agcgaatcag gaatcgatcg
     3481 agaacaatcc gttcgcaccc tccggccagc aggatatttc agcgctgtat accgggctga
     3541 aggttacgcg cggcatctgg gaggcagatt tcaatctcaa ctacacacgt aacaggatca
     3601 cagggtacaa gcccgcctgc gattcacgcg ttatctgcgt gccacagggt agctacgata
     3661 ttgacgataa agagggtggc ttcaaccctt cagttcagct ttctgctcag gtaacaccat
     3721 ggcttcagcc gttcattggc tacagcaaat ccatgcgcgc cccgaacatc caggagatgt
     3781 tcttctctaa ttcaggaggc gcatccatga acccattcct gaagcctgaa cgtgcagaaa
     3841 cctggcaggc gggttttaac attgatacca gagatttact ggtcgaacag gatgccctgc
     3901 gctttaaggc tctggcgtac cgcagcagga tccagaacta catctacagc gagtcttatc
     3961 tggtttgttc tggaggtcgt aaatgcagta tggctgaggt gattggcaat gactgggagg
     4021 gcattagcga tgaatacagc gacaatatgt acatctacgt taactcggca agcgacgtta
     4081 ttgcaaaggg cttcgaactg gagatggatt atgatgcagg ttttgctttt ggccgactct
     4141 ctttcagcca gcagcaaaca gaccagccaa cctccatcgc cagcacctac tttggcgcag
     4201 gggatatgac cgaactgccc agaaaataca tgacgctgga tactggtgtt cgcttcttcg
     4261 ataacgcgtt gaccctgggc actatcataa aatacacagg caaggctcgt cgcctgtcgc
     4321 ctgattttga gcaggacgaa cataccggcg caataatcaa acaggatttg ccgcagatcc
     4381 caacgattat cgatctctat ggtacttacg agtacaaccg caacctgaca ctgaaacttt
     4441 cggtacaaaa cctgatgaac agagattatt cggaggcgct gaataagctc aacatgatgc
     4501 caggtcttgg tgacgagagc cacccagcca attccgcgcg tggcagaaca tggatatttg
     4561 gcggggacat tcgtttctga tgacaatttc cgccaggttc agacaatacg ttttctctct
     4621 tatgtcaatt ttattgcagg aacgaaaaat gaatattttc actttatcca aagcaccgct
     4681 atacttgtta atttcactat ttttacccac gatggccatg gctatcgatc cacctgaacg
     4741 cgaactttcg cgatttgccc tgaaaacgaa ttaccttcag tcccctgatg aaggcgtcta
     4801 tgaactggcg tttgataatg ccagtaaaaa ggtgtttgca gcagtcaccg atcgtgtaaa
     4861 tcgtgaagcc aataaaggct atctgtattc gtttaattca gattcgctga aagtcgaaaa
     4921 taaatacacg atgccatacc gggcattttc gctggcgata aatcaggata aacatcagct
     4981 ctatatcgga cacacccagt cagcgtccct gcgtatcagt atgtttgaca ccccaaccgg
     5041 taaactggta agaaccagcg acaggttaag ttttaaagcg gcaaacgctg cagattcgcg
     5101 ttttgagcat ttgcgccata tggtttacag ccaggattcc gataccctgt ttgtgagtta
     5161 tagcaatatg ctgaaaacgg ccgagggcat gaagcctctg cataagctgt taatgctcga
     5221 cgggacgacg cttgccttaa aaggcgaggt taaggatgct tacaaaggta cagcgtatgg
     5281 tctgacgatg gatgaaaaaa cacagaaaat ctacgttggc ggaagagatt acatcaacga
     5341 aattgatgcg aaaaatcaga cgctgctgcg taccatcccg ttgaaagatc cgagaccaca
     5401 aatcacaagt gtgcagaatc tggcggtgga ctccgcttct gaccgtgcct ttgtggtggt
     5461 attcgaccat gacgatcgtt ccggtacaaa agatggcctc tatatttttg acttacgcga
     5521 cggtaaacag cttggctatg tgcacacagg agccggagct aacgcggtga aatacaatcc
     5581 gaaatataac gaactgtatg tcaccaactt tactagcggc accatcagcg tagtggatgc
     5641 caccaaatac agcatcaccc gtgaatttaa catgccggtc tacccaaacc agatggtgtt
     5701 gtcggacgat atggataccc tttacattgg catcaaagaa ggctttaacc gcgattggga
     5761 tcctgatgtg tttgtggaag gagctaaaga acgtattctg agcattgatt tgaaaaagtc
     5821 gtgatcgggg gaaaaaacat gggataaaaa ctccgttatt ccactaaact ggccggacta
     5881 ggaaaagcaa acatctctgt cctgccggta cgcctgttac gccgctccat gaggctgagg
     5941 aatctccaga aaacgagaca ggcacagcat tgtacgatca actgatttgt gccaataatt
     6001 caatgaaatc acagctatgc ctgttcacgt ataggaaatt gaaaaactaa gcgataccac
     6061 tgtccgcgtc ttatgcctcg tgcggtaagc tgtttttcac atcacgccgg tgtttttttc
     6121 tttctgtgga cggttttaat actgtaatcg gccgtttttt atacttaccc cgattcccgg
     6181 gcgcacttat cctgcatcac cccgggatat acctcagcag actgatatcc cgcgcgtttt
     6241 tcttcagccc ctccacattc agtcccgcga tggcctggcg gtataccatc cggccgccac
     6301 acagcacgca ttcgaacggg tcacgactca ggaactgttt caccatttgt gcatagcaca
     6361 ctttcgccac tggttccggt ttatcctgtt tttataacgg taattgcaga ccatgacaaa
     6421 ccgcagccgt caggctgcct actcgagtca caaggctctc tgtgtccgat atgccggcag
     6481 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
     6541 ggtgaagaat ta
//
LOCUS       DAJTNA010000200         6552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTNA010000200 DAJTNA010000000
VERSION     DAJTNA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.203"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..513)
                     /locus_tag="OO161_003552"
     CDS             complement(<1..513)
                     /locus_tag="OO161_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HCR5464481.1"
                     /translation="MGNYIRPLSDAVFTIASDDQWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGCGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWCSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="OO161_003553"
     CDS             complement(759..1211)
                     /locus_tag="OO161_003553"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5464482.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OO161_003554"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OO161_003554"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5464483.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OO161_003555"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OO161_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5464484.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="OO161_003556"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="OO161_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(5027..5524)
                     /gene="rayT"
                     /locus_tag="OO161_003557"
     CDS             complement(5027..5524)
                     /gene="rayT"
                     /locus_tag="OO161_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5464485.1"
                     /translation="MSEYRRYYIKGETWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5700..6449)
                     /locus_tag="OO161_003558"
     CDS             complement(5700..6449)
                     /locus_tag="OO161_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5464486.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHIGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARWILTEETIL"
BASE COUNT         1586 a         1702 c         1691 g         1573 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcta gtgcaaacag
       61 ttcagcgttg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcacc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccagatc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccata ccgcagccga tatcattgcc
      301 gaccagtgcc ggataaaaac gaccaacaga gaagaacgct gcgccaatcg gatagccgca
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccactggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tataattgcc catataccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgttg cgactagaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cttgcgttat tttcattgca agctggattt aatgttgctg tttataacgc
      781 atataaaaat taataaacca ctctccccga cattcaacgc gttgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgcttg cgtccaccgt aagttcggat
      901 tcagacttaa tcaaaggttt taacaaagcg ctggcaaccc cacggcgggt gtattcaggg
      961 tcaacaaata acatatcgat atagtgttca acgcaggtaa taaagccgac cagttttgca
     1021 ttaatgactg caacccgcac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtggt gaataatgct gactggcagt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtc tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcatcaaa ctttaatttc accccctggc gagcaatcag taaatcaggt ttcacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ccgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcggtc acccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
     3121 aaaggggccg agcgtatttc cgaggtttca gcccgcttta ccctggatgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatattaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga aggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 cttatcgagc ctatcagttt aagcctcggc tacaagctgg tggcgctggt cgataaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcaac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gccctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtct
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagt
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgctcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatcca gcattggtgc acaactgccg gatgcggcgt gaacgcctta tccggcctac
     4861 cgatccagca caggtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataaccgcc ggatgcggca taaacgcctt atccggccta ccgatccggc acaggtttgt
     4981 aggcatgata agacgcgcca gcgtcgcatc aggcatctgc gcataattat aaaatgatac
     5041 gctcgccagc atttatatcc gttacgtccc ccgcccaatc gatgggatat aaccctcgcg
     5101 cgacatcgcg atggaacgtt gagaatggcc aatcactcac ttgctttacc caaccatgct
     5161 ttactggatt tatataaata taatcaacat gatgcctata atctttggta ttgcggatgg
     5221 cgtgctccca aaaacgtggt tgccagatat ttttcaatcc acaagcatgg gtaaattgct
     5281 ttttaatttc ccgccagcga gaggaaaaat catcatcgcc ttcaggtaat gtccatatgc
     5341 aatgcatatg ctctggcaaa acgacccagg cgttgatttc aaaaggcctg tctcgcttaa
     5401 ctttaataat ggcgttacgg agcatctggt actgggtggt caagagttgg cttcgacgat
     5461 tgcgtaaatt caccgtgaaa aaccaggttt cccccttgat gtaataacga cgatattcag
     5521 acatggcgac tcctttttct caacggtaag aagaaaagga aaggtcggca atgaggtgat
     5581 ggaataacag gaaagcggct cgcaaaaacg cacagatttg taggcatgat aagacgcgtc
     5641 agcgtcgcat caggcatctg cgcaccaccg ccggatgcgg cgtaaacgcc ttatccgtcc
     5701 tacaaaatcg tctcttccgt caatatccac cgcgcgccca aaaaatgatc ctgccagaaa
     5761 ggttcagcta accgacttac ccgaatggtt tcccccgtgc gcggcgactc aataaactga
     5821 ccgtctccca gatacacgcc aatatggtcg gcaatttcac ggctgtggat atggaaaaac
     5881 agcaaatctc cccggcgcag gtcgttattt gccacaatgg ttgcccgatg gtagtgatac
     5941 atctcattag ccgtgcgcgg gagcttagcc tcaaggatct tgttgtaggc ataaaaaacc
     6001 aacccgctac agtcgaagcc ttgatcgggc cgcgtgccgc cccagacgta aggcttgcca
     6061 agctgttgct ccagacggtg aatcgccact tcggtgatat tatgcaggtg gttgctgctg
     6121 aggaaatgat tgttttctac caacgcctgc cagcgtctgt cacttgcctt gagcggcgca
     6181 ggaaaccatt ctggatgctg ttttatctgc tcccggttgt gctggcgtag cgcccttctg
     6241 ctttcggcat tgccttccac aatgtagctg gcctgttttt tcagatgggt ttggtactgt
     6301 tttaataaac gcgcacggtt ttgcatccgc tggcgggcgg cgtagctgaa agagatatgt
     6361 gatgtggtgg cgctggcgag taaagggagc aaaacgcata acgaaaacac gcctggatgc
     6421 agaaatctgc cgagcaagaa cgaagacatg aaggacataa accggctgaa tgaaggtgag
     6481 aattttaaaa gaccggagga gtaaacggga ttattaattg cttatttgca tattactatt
     6541 gaggtttcat aa
//
LOCUS       DAJTNA010000201         6531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTNA010000201 DAJTNA010000000
VERSION     DAJTNA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.204"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..858
                     /gene="ag43"
                     /locus_tag="OO161_003559"
     CDS             <1..858
                     /gene="ag43"
                     /locus_tag="OO161_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5464487.1"
                     /translation="HDNNGGIARGATPESSGSYGLVRLEGDLLRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRHSMKASSD
                     NNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGIQAGYAHSISGSSAEGYN
                     GQATLNVTF"
     gene            930..1163
                     /locus_tag="OO161_003560"
     CDS             930..1163
                     /locus_tag="OO161_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5464488.1"
                     /translation="MPGCTSRLLPEGPFSREQVVAVTTVYRNVFTEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNVWLASEGLLRQ"
     gene            1254..2072
                     /locus_tag="OO161_003561"
     CDS             1254..2072
                     /locus_tag="OO161_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5464489.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGDVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            2164..2649
                     /locus_tag="OO161_003562"
     CDS             2164..2649
                     /locus_tag="OO161_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5464490.1"
                     /translation="MTTFSHSSTTPSVSVTAASGNNQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            2664..3140
                     /locus_tag="OO161_003563"
     CDS             2664..3140
                     /locus_tag="OO161_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5464491.1"
                     /translation="MQQISFLPGEMTTRERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            3227..3448
                     /locus_tag="OO161_003564"
     CDS             3227..3448
                     /locus_tag="OO161_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5464492.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3528..3896
                     /locus_tag="OO161_003565"
     CDS             3528..3896
                     /locus_tag="OO161_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5464493.1"
                     /translation="MSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKT"
     gene            3986..4363
                     /locus_tag="OO161_003566"
     CDS             3986..4363
                     /locus_tag="OO161_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5464494.1"
                     /translation="MKTLSDTHIREASRCPSPVTVWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTNSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKYQEAKR"
     gene            4360..4539
                     /locus_tag="OO161_003567"
                     /pseudo
     CDS             4360..4539
                     /locus_tag="OO161_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4588..4782
                     /locus_tag="OO161_003568"
     CDS             4588..4782
                     /locus_tag="OO161_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCR5464495.1"
                     /translation="MPLTTETALDILIAWLQDNIDCESGIIFDNDENKTDSVTLLPCI
                     KQVRQDVRTLRHMQLLHQNR"
     gene            4867..5712
                     /locus_tag="OO161_003569"
     CDS             4867..5712
                     /locus_tag="OO161_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR5464496.1"
                     /translation="MSDITASRPEVVNGHTAVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINIFRGLS
                     WNYKNNSPCKFGSKIIVNNLVRWDRWGFHLNNGPQADRLADLERMLHLFSGKPIPDNR
                     ENITIRLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIVAKHYPG
                     QLSAI"
     gene            complement(5812..5887)
                     /locus_tag="OO161_003570"
     tRNA            complement(5812..5887)
                     /locus_tag="OO161_003570"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5852..5854),aa:Ile,seq:cat)
     gene            6012..6518
                     /gene="mug"
                     /locus_tag="OO161_003571"
     CDS             6012..6518
                     /gene="mug"
                     /locus_tag="OO161_003571"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR5464497.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         1583 a         1754 c         1728 g         1466 t
ORIGIN      
        1 cacgataaca acggtggtat tgcccgtggg gccacgccgg aaagcagcgg cagctatggc
       61 ctcgtccgtc tggagggtga cctgctcaga acagaggttg ccggtatgtc tgtgaccgcg
      121 ggggtatatg gtgctgctgg ccattcttcc gttgatgtta aggatgatga cggctcccgc
      181 gccggcacgg tccgggatga tgccggcagt ctgggcggat acctgaatct ggtacacaca
      241 tcctccggcc tgtgggctga cattgtggca cagggaaccc gccacagcat gaaagcatca
      301 tcggacaata acgacttccg cgcccggggc tggggctggc tgggctcact ggaaaccggt
      361 ctgcccttca gtatcactga caatctgatg ctggagccac aactgcagta cacctggcag
      421 ggactctccc tggatgacgg ccaggataac gccggttatg tgaagttcgg gcatggcagt
      481 gcacaacatg tgcgtgccgg cttccgtctg ggcagccaca acgatatgaa ctttggtaaa
      541 ggcacctcat cccgtgacac cctgcgcgac agtgccaaac acagtgtgcg tgaattgccg
      601 gtgaactggt gggtacagcc ttctgttatc cgcaccttca gctcccgggg agacatgagc
      661 atgggtacag ccgcagccgg cagtaacatg acgttctcac cgtcccggaa tggcacgtca
      721 ctggacctgc aggccgggct ggaagcccgt gtccgggaaa atatcaccct gggcattcag
      781 gccggttatg cccacagtat cagcggcagc agcgctgaag gctataacgg tcaggccacg
      841 ctgaatgtga ccttctgatt aatcaggtca ctccccttca ggccccggtc attatgaccg
      901 gggcctttta ctttcaggga gtaacagata tgccgggttg tacatcacgt ttattgcccg
      961 aaggcccttt ctcacgcgaa caggtggttg ctgttacaac agtttaccgc aatgtgttta
     1021 ctgaagatga ccagggaacg catttccggc tggttatccg taatgctgaa ggtcagctac
     1081 gctggcgctg ctggaatttt gagcccgatg ccggcaaaca acttaatgtg tggctcgcca
     1141 gtgaagggct tctcagacaa taaacgtcat tcatttcccc catcaggccg cgtcttctcc
     1201 gggagacgcg gctttttcat ttataccgct aactcattca taaggaataa atcatgcgat
     1261 tagccagtcg ttttggttat gcgaaccaga tacgccgtga ccgcccctta actcgtgaag
     1321 aactgatgca ggtcgtcccc agcgtattcg gggaagagaa acacacatcc agaagcgaga
     1381 actatacctg gatccccacc atcaccgtgc tggaaagcct gcagcgggaa ggcttccagc
     1441 cattcttcgc ctgccagacc cgcgtgcgcg acccgggccg ccggggatac accaaacaca
     1501 tgctgcgtct gcgccgtgac ggggagataa acggacaaca tgttcctgaa atcatcctgc
     1561 tgaactccca tgacggtacc tccagctacc agatgctgcc gggttacttc aggttcgtct
     1621 gccagaacgg atgcgtctgt ggtcagtctc tgggggaagt gcgtgttcca caccggggag
     1681 acgtagtgga gaaagttatc gaaggggctt acgaggtggt gggggttttt gaccggattg
     1741 aggagaagcg tgatgccatg cagtctctgg ttctgccgcc accggcacgc caggcgctgg
     1801 cacaggcggc actgacttac cgttatggtg acgaacatca gcccgtcacc accgctgaca
     1861 ttctgacgcc acgacgccgg gaggattacg gtaaggacct gtggagtgct tatcagacca
     1921 tccaggaaaa tatgctgaaa ggcgggattt ccggtcgcag cgctaaagga aaacgtatcc
     1981 acacccgtgc cattcacagc atcgacaccg atattaagct caaccgcgca ttgtgggtga
     2041 tggcagaaac gctgctggag agcatgcgct gatgctgttt ccctgaaagt acagccctga
     2101 ctcacgtctg taccagtccc ttggcattct tccattcatt tactttttat aaggaatcat
     2161 ctcatgacaa ccttttcgca tagctccacc acaccttctg tttccgtaac cgctgcatca
     2221 gggaataacc agtctcaact ggttgccacc atagtccctg atgaacagcg catcagcttc
     2281 tggccgcagc actttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc
     2341 tggatggacc gtctgtgtga agactactgc ggtggtatct ggaatctgta caccctgaat
     2401 aacggcggcg catttatggc gcccgaaccg gatgaagatg atggagaaac ctgggtactg
     2461 ttcaatgcca tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg cattgccgcc
     2521 tgtctgatga cgtacagcca tcatgcctgt cgtacggaga attatgccat gacggtccat
     2581 tattaccggt tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc
     2641 attgactgaa aggggccgga ataatgcaac agatttcctt tctgcccgga gaaatgacaa
     2701 ccagagagcg cagcctcatt cagcgggccc tgaaaaccct ggaccgccat cttcatgagc
     2761 ccggcgtggc cttcacctcc acgcatgcgg cccgggaatg gctgattctg aacatggcag
     2821 gactggagcg ggaagagttc cgggtactgt atctgaacaa ccagaatcag ctgattgccg
     2881 gtgaaaccct cttcaccggc accatcaacc gcacggaagt tcatccccgg gaggtgatta
     2941 aacgcgcgct gtaccacaat gccgctgccg tggtgctggc acacaatcac ccgtccggtg
     3001 aagtcacacc cagtaaggca gaccggctta ttacggaacg tctggttcag gcgctggccc
     3061 tggtggatat ccgggtaccg gaccatctga tagtcggtgg cagccaggta ttctcctttg
     3121 cggaacacgg tctgctttaa cccgtcaccg tcacaatcac cttcatatca cttcaatttc
     3181 tctttctcag ctgtttctta ctttcacatt caggaggact attctcatga aaatcatcac
     3241 ccgtggtgaa gccatgcgta ttcaccgtca gcatcctgca tcccgtcttt ttccgttctg
     3301 taccggtaaa taccgctggc acggtagcac ggatacatat accggccgtg aagtacagga
     3361 tattcccggt gtgctggctg tgtttgcaga acgccgtaag gacagttttg gtccgtatgt
     3421 ccggctgatg agcgtcaccc tgaactgagt ctggaagggc attcagaaga gcagaattat
     3481 caccaccacc ggaccattct taaccaattt tctgtgagga ttttatcgtg tcagacacac
     3541 tccccgggac aacgcatccc gacgataaca acgaccgccc ctggtggggg ctaccctgca
     3601 ccgtgacgcc ctgttttggg gcacgtctgg tgcaggaggg taaccggttg cattaccttg
     3661 ccgaccgcgc cggtatcaga ggccggttca gcgacgcgga tgcgtaccat ctggaccagg
     3721 cctttccgct gctgatgaaa caactggagc tcatgctcac cagcggtgaa ctgaatcccc
     3781 gccatcagca taccgtgaca ctgtatgcaa aagggctgac ctgcgaagcc gacaccctcg
     3841 gcagttgtgg ctacgtttat atggctgttt atccgacgcc cgaaacgaaa acgtaaatca
     3901 tcagaataat cttctgctcc ggcctggtgt tttcaccacg ccactttccc atttttcatc
     3961 tctgcatatc aggaaaatct tcagtatgaa aacattatcc gacacacaca tacgggaggc
     4021 atcgcgctgc ccgtctcccg tcaccgtctg gcagacactg ctcacccgac tgctggacca
     4081 acactacggc ctcacgctga atgacacacc gttcgctgat gaacgtgtga ttgagcagca
     4141 tattgaggca ggcatttcac tgtgcgatgc ggtgaacttt ctcgttgaaa aatacgcgct
     4201 ggtacgtacc gaccagccgg gattcagcgc ctgtaccaac tctcagttaa taaacagcat
     4261 cgatatcctc cgggctcgcc gggcgactgg cctgatgacc cgcgacaatt acagaacggt
     4321 aaataacatt acccagggta agtatcagga ggcgaaacga tgaaactggc cctgacgctg
     4381 gaagctgaca gcgttaacgt acaggcactg aacatggggc gaatcgtcgt tgacgtcgat
     4441 ggtattgagc tcgctgaact gattaacgaa gtcgctgaaa acggttattc actccgcatg
     4501 gtggatgaac gtggcgctac cgaaacaccg gcgacttctg ccagccctca tcagttgccg
     4561 gaagacaata cagtgataca gagcgcgatg ccattaacca ctgaaacagc gctggatatc
     4621 ctcattgcgt ggctgcagga caatatcgac tgcgagtccg gaattatttt cgacaacgac
     4681 gagaataaaa cggattcagt tacactgtta ccctgtatta aacaggtgcg acaggatgtc
     4741 cgtaccctgc gccatatgca acttctgcac cagaaccggt gaatctcact catcagactt
     4801 cattccactg acgccagcct gaacagggct ggcgttttca tttatctgca aaaaggaata
     4861 tcgattatgt ctgatatcac agcctcccgt ccggaagtgg tcaacgggca tacggccgtt
     4921 atctgctcaa ccagtatccg ccacattctg gctgtacgaa aaagtacact gctgcaaatc
     4981 gacacactta tccggcaact ggctgaaatc tcagcaatga cagaaagtat tggcggtaaa
     5041 accgcactgg actgggccat gaaacagaat tttcgctgcg gctgctggct gatggagaaa
     5101 cctgaaacgg caatgaaagc catcacccgc aaccttgacc gcgaaatctg gcgtgacctg
     5161 atgcaacgtt ccgggatgct gtcattaatg gatgcgcagg cccgtgatac atggtaccgg
     5221 tcactggagt acgataattt tccggaaatc agtgaagcga acattctgag cacatttgaa
     5281 caactgcacc agaataagga tgaggtgttt gagcgagggg taatcaatat tttcagggga
     5341 ctgagctgga attacaaaaa caattcaccc tgtaaatttg gcagtaaaat tatcgtcaac
     5401 aatctggtga ggtgggacag atggggattt catctgaaca acggcccaca ggccgatcga
     5461 cttgccgacc tggaaagaat gctgcatctg ttcagcggca aaccgatccc cgacaaccgg
     5521 gaaaacatca ctattcgtct ggatgatcac atccagtctg ttcagggtaa agaggactat
     5581 gaagatgaga tgttcagcat cagatacttt aagaagggct ccgcgcatat cacgttcaga
     5641 aaaccagaac tggttgatcg gctaaatgac atcgttgcga agcattatcc ggggcaacta
     5701 tcagccatct gagtcagatg gagaaaaagc ttacctgacc cattacctga cccaattcaa
     5761 aggcaaaaga aaaagggtta gctgatttat tagctaaccc tttgttgtat atggtggccc
     5821 ctgttgggtt tgaaccaacg accaagcgat tatgagtcgc ctgctctaac cactgagcta
     5881 aggggccatg gtagcggatt ataaagtaac tccgtgtcgc aatccagcca ttaccgtgcg
     5941 cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg tgatgttaga
     6001 aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg ttttgcggta
     6061 tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatcca gcaaatcgct
     6121 tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg caggaggcac
     6181 agcatctgct ggattatcgt tgtggcgtca ccaaactggt agaccgtcca acggtgcaag
     6241 ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt gaaaaaattg
     6301 aagattatca gccgcaggcg ttggcgattc tgggcaaaca agcatatgaa cagggattca
     6361 gccagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg cagatttggg
     6421 tgctgccaaa tcccagcggt ttaagtcgcg tttcactaga gaaactggtt gaagcgtatc
     6481 gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa aacgccaccg a
//
LOCUS       DAJTNA010000202         6511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTNA010000202 DAJTNA010000000
VERSION     DAJTNA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.205"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..138
                     /locus_tag="OO161_003572"
     CDS             <1..138
                     /locus_tag="OO161_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5464498.1"
                     /translation="ATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAAWVH
                     R"
     gene            163..342
                     /locus_tag="OO161_003573"
                     /pseudo
     CDS             163..342
                     /locus_tag="OO161_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            868..2046
                     /gene="ltrA"
                     /locus_tag="OO161_003574"
     CDS             868..2046
                     /gene="ltrA"
                     /locus_tag="OO161_003574"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5464499.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2129..2308
                     /locus_tag="OO161_003575"
                     /pseudo
     CDS             2129..2308
                     /locus_tag="OO161_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2338..2820)
                     /locus_tag="OO161_003576"
                     /pseudo
     CDS             complement(2338..2820)
                     /locus_tag="OO161_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3076..4056)
                     /locus_tag="OO161_003577"
     CDS             complement(3076..4056)
                     /locus_tag="OO161_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5464500.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVMKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4056..5255)
                     /locus_tag="OO161_003578"
     CDS             complement(4056..5255)
                     /locus_tag="OO161_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5464501.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5577..5915)
                     /locus_tag="OO161_003579"
                     /pseudo
     CDS             complement(5577..5915)
                     /locus_tag="OO161_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5982..6511)
                     /gene="istB"
                     /locus_tag="OO161_003580"
                     /pseudo
     CDS             complement(5982..6511)
                     /gene="istB"
                     /locus_tag="OO161_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT         1757 a         1505 c         1556 g         1693 t
ORIGIN      
        1 gccacactca cgtgggtgga ctggtataac aatcgacgat tgctggaaag gctgggccat
       61 actcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
      121 gcctgggttc acagataaaa cactctccag gaaacccggg gcggttcagt tgctgaactg
      181 ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa agtccatacc
      241 gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac tgcccggctg
      301 tgggtctacg ttcgtgatga ccgtaacgcc gcttcggaaa ttgcagcata agttatccat
      361 gctctgaggt gggtggaggt gggccctccg gtcagggttt acaggagcac tgaccaaacg
      421 acccggtgct gcttcattct aaggaatgag gtggtgagcg actcgggaaa aggcagaggt
      481 ctactgtcac gtggggttcg ggaagctgaa cgaaagtaaa ctgtccgatg aagcatcgaa
      541 atcttagatg atgtcaaaat ggggccgtac agccgtcccc tgataagcgc agaagacacc
      601 tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc agaccatctc
      661 agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggcc ccattcgtag
      721 tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca ttcattgcag
      781 aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt gaaataccaa
      841 aagcactggt ctgggcatcc tatctggatg tgcgtcgcaa caagggagcc ccccggatgc
      901 gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata caagatctgg
      961 aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg gatcccgaag
     1021 tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat tgctcaggga
     1081 gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga ttcatatggt
     1141 taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg gtgctggcgt
     1201 tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgaa acatgatctg
     1261 gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta ctgtcgacgc
     1321 tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg aacacgaggc
     1381 actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca ctatgcattt
     1441 gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc tgacgatatt
     1501 gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt atcggagcgc
     1561 ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta cattgacacg
     1621 tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt caaagtgcgt
     1681 acgctgaaga atttcaaagg cgaacggtac cgaaaatgca tgccgggtgc gtcaaatgca
     1741 gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc aacagctgag
     1801 agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat cgagtactac
     1861 ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca gtcacgtctg
     1921 ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg aaagctgacg
     1981 ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg tgcatcaaat
     2041 gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag cagccgggga
     2101 gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg aacatgcggc
     2161 agtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag aaaaatggct
     2221 gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg atctgttgcc
     2281 ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag ccgcttactt
     2341 ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa gaagtccacg
     2401 aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt
     2461 ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc catgttggca
     2521 ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct gataagcttt
     2581 aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt cccgggccca
     2641 gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt gcaccagcca
     2701 gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat gagcattgat
     2761 actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct gttcagacat
     2821 ggtatcccgc ttacttttgt ggttaaagtt tatccatcat ggcgcaattg aaggctgccg
     2881 tgaaagaggg ggaatactat ctcatcggtt actgttaaac ggctaagaga aactatcttt
     2941 ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa
     3001 acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggtg agttgctggc
     3061 gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag caatcatacg
     3121 atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc
     3181 ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca aagttacaac
     3241 cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta taagccgcat
     3301 aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa gctcaatact
     3361 cggcgagata ttcttcataa cttcatcaat agatatatca cccagcctca gtcgttcact
     3421 taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag gaaaaagaga
     3481 cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag ctgccgacaa
     3541 tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac gtactaaacg
     3601 caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg ccagatgctg
     3661 agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa ccttgcatag
     3721 caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc cagtccgaat
     3781 cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc
     3841 cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag tttgagtgtc
     3901 cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag tattgagggt
     3961 aaatacatga actggagccg atatttcttc agtattattc aggatatttg tattcagcgt
     4021 acggccaata gtcggtcggt gctttctgtt ttccatcagt agttagccct tataaattca
     4081 atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt tttcaaagct
     4141 tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa cgactcgccg
     4201 cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc
     4261 tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt cgacatgaag
     4321 ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg tacaagttca
     4381 ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt
     4441 gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag atgcgggcct
     4501 gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt ctcacgcaat
     4561 atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca gtttgatgca
     4621 ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac tgacggaacg
     4681 ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg cggtgcggtt
     4741 gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga tgactgggga
     4801 tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg agcacgcagg
     4861 gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag actaccgatg
     4921 aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg atgtgcatag
     4981 atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc tgggcgtttt
     5041 tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct tgcgcgcgtt
     5101 aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac atcagcctga
     5161 aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt atctgcacgc
     5221 tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt
     5281 taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtgca tattgtacat
     5341 gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa tattactgta
     5401 agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc agttcaccac
     5461 cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt
     5521 ccggtaacgg acgacgatga cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag
     5581 tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc cacggaacgc
     5641 gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt ccggttgtta
     5701 cgttcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg cacttctctg
     5761 gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt caacagggct
     5821 aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa cacaccgcca
     5881 gttttagtgt tgtacgcata ccagagccag tgttgtgtca acgacggatg aaaagtgatc
     5941 cacttatatc tccaccaacg gcccaatatt gatccaccgt tttactcagg attagcttct
     6001 gctataaccc cggcctttcg tttctgtctg agtcgatagc tttctccttt gatttgaacg
     6061 acatgtgagt ggtgtaagat acggcccagc atcgctgagg tcagtgctgc atcaccggcg
     6121 aacgtttgat cccactgccc gaacggtaga ttggatgtca ggatcattgc gctcttttca
     6181 taacgtttag cgatgacctg gaagaacagt tttgcttctt tctgactgaa cggcagatag
     6241 cctatttcat caatgatgag caggcggggg gccattactc cacgctgaag cgtcgtttta
     6301 taacggccct gacgttgtgc catagataac tgaagtaaca gatctgctgc tgttgtgaag
     6361 cgaacttgga tacctgcacg gactgcttca tagcccatcg ctattgccag atgggttttc
     6421 cccacacctg atggccccag taatacgata ttttcattac gttatatgaa gctgagtgag
     6481 cgtaacgact ggagttgctt ctgcggtgct c
//
LOCUS       DAJTNA010000203         6326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTNA010000203 DAJTNA010000000
VERSION     DAJTNA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.206"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..524)
                     /gene="yneK"
                     /locus_tag="OO161_003581"
                     /pseudo
     CDS             complement(1..524)
                     /gene="yneK"
                     /locus_tag="OO161_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            complement(602..1483)
                     /locus_tag="OO161_003582"
     CDS             complement(602..1483)
                     /locus_tag="OO161_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5464502.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            1584..2972
                     /gene="sad"
                     /locus_tag="OO161_003583"
     CDS             1584..2972
                     /gene="sad"
                     /locus_tag="OO161_003583"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR5464503.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            3036..3962
                     /gene="glsB"
                     /locus_tag="OO161_003584"
     CDS             3036..3962
                     /gene="glsB"
                     /locus_tag="OO161_003584"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5464504.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3962..4321
                     /locus_tag="OO161_003585"
     CDS             3962..4321
                     /locus_tag="OO161_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5464505.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            4460..5878
                     /locus_tag="OO161_003586"
     CDS             4460..5878
                     /locus_tag="OO161_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5464506.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            6105..>6326
                     /locus_tag="OO161_003587"
     CDS             6105..>6326
                     /locus_tag="OO161_003587"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5464507.1"
                     /translation="MKTLNRRDIPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIR"
BASE COUNT         1523 a         1441 c         1676 g         1686 t
ORIGIN      
        1 tggtgtatgc gcttttttga tggggacgcc aaggtggatc attgtaaatt gttcaataaa
       61 atcattcaga aacagaaaga gcacacgctc gacgccggtg tgaaatacgc gccccatagt
      121 gacaggcttt gggttgggag cgcaaaaata ttaatttctc catcatggcg aaaattatct
      181 aaataaatag ggcttaaatt tattactacc tgaaattcaa tattttcttt gtgtcttcca
      241 ttcttgacgg gataagtgag tttaaactga accttttcat tgtatagtgt gtcgttaccc
      301 cgaaaagaag tcctctgaat atatgataag ttatcaaggt tgttaaatat cttttttgtg
      361 gctgagttgg tgtttaaaga cgtgcgccac tcttcatcac atattatggt actgagtgca
      421 tcaataaaat tctttaaaac ttcttttacc tgaggtgcaa tattacgtac gggaaaatca
      481 tcaggaagag atatgtaatt actgatgctt actggcgtaa ccatatttgc cccatatagt
      541 cataagtcat cggaacatca ctatataggg catttgagct gcaagaacta tcaaaaatag
      601 ctcattggta accctgtttt gccgagtcag gcacatccag tagttgaata aatgcctcaa
      661 gcggacgcgt tttcgcacca cgacgccaga ccagccaggt tgttaaccaa cgccattgct
      721 cagctaacgg ccacgcttca acctggtgat gccccggcat actttccagc atagagcgtg
      781 gaataagcgc aatgcctgct cctgcgacca cacaggccaa catcccgtga tacgactcca
      841 tctcgtgaat agttcccgga gcggcaccgt cagcatgaaa ccagctctcg aagtggcgac
      901 gatacgaaca attggcgcgg aaggcataaa tgttactgcc gttaacctga ctggcgcgga
      961 ttactggcgc atgaccttgt ggcgtgacga tcatcagctc ctcgcggtat accggtatcc
     1021 cgtcaatggc agtatggtta atgggcccat caataaatgc cgcattcagt tttccctcca
     1081 gcactccttc cagcatagtg ccggaagggc cggtggaaag tgaaaactga attttgggat
     1141 aacgatggtt gaattccgcc agcgtggccg gaatgcgcac tgctgcggtg ctttccagcg
     1201 aaccaagaga aaataaacct tgcggctcat cgccagcgac aacgctccgc gcttcatcca
     1261 ctaacgcgag aatttgttgg ctatagcgta aaaagttatg cccggcagga gagagacgta
     1321 aacgctgatt ctcgcgaata aacagatcta cccccagttc tgtttccagc tggcgtagac
     1381 gggtagtgag attcgacggc acgcgaagca ctattgctgc tgcctgggtt atgctgccag
     1441 cttcggcaac cgcgttgaac atctccagtt gggtcagatc cataatattc tcgattcgtg
     1501 aataagtggc ttaatattat tcattttaaa gcaagagtaa atctgcgtat cttcatacca
     1561 tgactcataa aggagatacc ccgatgacca ttactccggc aactcatgca atttcgataa
     1621 atcctgccac gggtgaacaa ctttctgtgc tgccgtgggc tggcgctaac gatatcgaaa
     1681 acgcacttca gctggcggca gcaggctttc gcgactggcg cgagacaaat atagattatc
     1741 gtgctgaaaa actgcgtggt atcggtaagg ctctgcgcgc ccgtagcgaa gaaatggcgc
     1801 aaatgatcac ccgtgaaatg ggcaaaccaa tcaatcaggc gcgcgctgaa gtggcgaaat
     1861 cggcgaattt gtgtgactgg tatgcagaac atggtccggc aatgctgaag gcggaaccta
     1921 cgctggtgga aaatcagcag gcagttattg agtatcgacc gttggggacg attctggcga
     1981 ttatgccgtg gaactttccg ttatggcagg tgatgcgtgg cgcggttcct atcattcttg
     2041 caggtaacgg ctacttactt aaacatgcgc cgaatgtgat gggctgtgct cagctcattg
     2101 cccaggtgtt taaagatgcg ggaatcccgc aaggcgtata tggctggctg aatgccgaca
     2161 acgacggtgt cagtcaaatg attaaagatt cgcgcattgc tgctgtcacg gtgaccggaa
     2221 gtgttcgtgc gggagcggct attggcgcac aggctggagc ggcactgaaa aaatgcgtac
     2281 tggaactggg cggttcggat ccatttattg tgcttaacga tgccgatctg gaactggcgg
     2341 ttaaagcggc ggtagccgga cgttatcaga ataccggaca ggtttgtgca gcggcaaaac
     2401 gctttattat cgaagaggga attgcttctg catttaccga acgttttgtg gcagctgcgg
     2461 cagccttgaa aatgggcgat ccccgtgatg aagagaacgc tctcggacca atggctcgtt
     2521 ttgatttacg tgatgagctg catcatcagg tggagaaaac cctggcgcag ggtgcgcgtt
     2581 tgttactggg cggggaaaag atggctgggg caggtaacta ctatccgcca acggttctgg
     2641 cgaatgttac cccagaaatg accgcgtttc gggaagaaat gtttggccct gttgcggcaa
     2701 tcaccgttgc gaaagatgca gaacatgcgc tggaactggc taatgatagt gagttcggcc
     2761 tttcagcgac catttttacc accgacgaaa cacaggccag acagatggcg gcacgtctgg
     2821 aatgcggtgg ggtgtttatc aatggttatt gtgccagcga cgcgcgagtg gcctttggtg
     2881 gcgtgaaaaa gagtggcttt ggtcgtgagc tttcccattt cggcttacac gaattctgta
     2941 atatccagac ggtgtggaaa gaccggatct gacgcgacat attcagctct gatatactcg
     3001 caggtctttt cagacctgcg gtccaggagt agaaagtggc agtcgccatg gataatgcaa
     3061 ttttagaaaa catcttgcgg caagtgcggc cgctcattgg tcagggtaaa gtcgcggatt
     3121 atattccggc gctggctaca gtagacggtt cccgattggg cattgctatc tgtaccgttg
     3181 acggacagct ttttcaggcc ggagacgcgc aagaacgttt ttccattcag tctatttcca
     3241 aagtgctgag tctcgttgtc gccatgcgtc attactccga agaggaaatc tggcaacgcg
     3301 tcggcaaaga tccgtctgga tcaccgttca attctttagt gcaactggaa atggagcagg
     3361 gtattccgcg taatccgttt attaatgctg gtgcgctggt ggtctgcgat atgttgcaag
     3421 ggcgattaag cgcaccacgg caacgtatgc tggaagtcgt gcgcggctta agcggtgtgt
     3481 ctgatatttc atacgatacg gtggtagcgc gttccgaatt tgaacattcc gcgcgaaatg
     3541 cggctatcgc ctggctgatg aagtcgtttg gcaatttcca tcatgacgtg acaaccgttc
     3601 tgcaaaacta ctttcattac tgcgctctga aaatgagctg tgtagagctg gcccggacgt
     3661 ttgtctttct ggctaatcag gggaaagcta ttcatattga tgaacctgtg gtgacgccaa
     3721 tgcaggcgcg gcaaattaac gcgctgatgg cgaccagtgg tatgtaccag aacgcggggg
     3781 agtttgcctg gcgggtgggg ctaccggcga aatctggcgt tggtggcggt attgtggcga
     3841 ttgttccgca tgaaatggcc atcgctgtct ggagtccgga actggatgat gcaggtaact
     3901 cgcttgcggg tatcgccgtc cttgaacaat tgacgaaaca gttagggcgt tcggtttatt
     3961 aatgcagtct ctcgatccac tcttcgcgcg tttatcccgt tcaaaatttc gctctcgctt
     4021 tcgtctgggc atgaaagagc gtcagtattg cctggagaaa ggcgcgccag tcatcgaaca
     4081 acatgcggcg gattttgtcg ctaaacgtct tgctccggca ttaccggcta atgacggtaa
     4141 gcaaacaccc atgcgcgggc atccggtgtt tatcgctcag cacgccaccg cgacctgctg
     4201 tcgcggttgt ctggctaagt ggcataacat tccgcagggt gtttcgttaa gcgaagaaca
     4261 acaacgttat atcgtggcgg taatctatca ctggctggtt gtacagatga atcagccatg
     4321 agaattgcgg aaaacgataa aatagcattc ccagggtacc tttatttgcc gccgctgtta
     4381 gcgaacatgc tatcctttat gagaatttac ttattacgtt gagataattg ccacccgcaa
     4441 ttgtatggac agataattaa tgcatgtaca ccccatttca actttccgtt tgttccagga
     4501 aggtcatctg ctacgtaata gcatcgctat ttttgcgcta accacgctgt tctattttat
     4561 tgttgcagag ttacggctgg ttcacgaact ttctcttttc tggccgctga atggcgtaat
     4621 ggcgggggtg tttgcccgct atgtctggct taatcgactg cattactatg cgatcagtta
     4681 tgtggcgatg ctggtttatg atgccataac caccgaatgg ggactggttt cactggttat
     4741 caatttctcc aatatgatgt ttattgttac cgtcgcctta ctggtcgtgc gggataagcg
     4801 tctggggaaa aataagtatg agccagtaag tgccttacgg ctatttaatt actgtctgat
     4861 tgccgcatta ttatgcgcta ttgtcggggc gattggttcg gtcagtattg atagtctgga
     4921 tttctggcct ttgcttgcgg actggttcag tgagcaattc tcaacgggcg tgttgatcgt
     4981 gccttgtatg ctgacgttgg ctattcctgg agtgctgccg cgctttaaag cagaacagat
     5041 gatgcccgcc atcgcgctta ttgtgtcggt tattgcctcg gtagtcattg gcggagcggg
     5101 gagtctggcg ttcccgctgc ctgcattaat ctggtgtgca gtgcgctata cgccgcaggt
     5161 aacatgtctg ttgacctttg tcaccggtgc ggtggaaatc gtactggtgg caaattcggt
     5221 gattgatatc tcggtcggtt cgccgttctc cattccggaa atgttctccg cacgtctcgg
     5281 tattgccacg atggcgatat gcccaattat ggtttctttt agcgtggcag cgatcaattc
     5341 gctaatgaag caagttgcgc tgcgagccga ctttgatttt ctgactcagg tttactcacg
     5401 gtccggtctt tatgaggcgc tgaaaagtcc atcgctgaaa cagacacaac atctgacggt
     5461 catgctgctt gatatcgact atttcaaaag cattaacgat aactatggac atgaatgtgg
     5521 cgataaagtg ttaagcgtgt ttgcccggca tattcagaag attgtcggtg ataaggggct
     5581 ggtggcgcgc atgggcggcg aagaatttgc tgttgcagtg ccgtcggtga atcctgtcga
     5641 tggtctgcta atggcggaaa aaatccgtaa aggcgttgaa ctgcaaccat tcacctggca
     5701 acaaaaaacg ctttatctca cggtaagtat tggcgtcggt agtggtcgcg catcgtaccg
     5761 aacgctgacc gatgatttta ataaattgat ggtcgaagcc gatacgtgtc tgtatcgctc
     5821 gaagaaagat gggcgcaacc gtaccagcac tatgcgttac ggtgaggaag ttgtctgaca
     5881 tctttattca ttccgcgcga tttgaatgca atgtgttcta ccactttatg atccaggaaa
     5941 aggcgtattt cctcgattaa accatgatcc gcgccacact tttctcataa aactgctcat
     6001 taatcatcag gagttgatca aaacaggggg tcggcgtatt aaagtttact aacatgtcgt
     6061 accaatttaa acggcttgta tgactaatca gaagggaacc cattgtgaaa acactaaatc
     6121 gtcgcgatat tcccggtgca cagtatccag aacgtatcat tcagtttggt gaaggtaact
     6181 tcctgcgcgc ctttgttgac tggcaaatcg atctcctgaa tgagcacacc gatctgaatt
     6241 ctggcgtggt cgttgttcgt ccgattgaaa catcattccc gccgtcactg agcacgcagg
     6301 atggtctgta caccaccatt atccgt
//
LOCUS       DAJTNA010000204         6296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTNA010000204 DAJTNA010000000
VERSION     DAJTNA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.207"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..216)
                     /locus_tag="OO161_003588"
     CDS             complement(<1..216)
                     /locus_tag="OO161_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5464508.1"
                     /translation="MSRKTQRYSTEFKTEAVKTVPENQLSISEGASRLSVPEGTLGQW
                     VTAARKGLNTPGSRTVAELESEVMQLRK"
     gene            complement(906..2615)
                     /locus_tag="OO161_003589"
     CDS             complement(906..2615)
                     /locus_tag="OO161_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5464509.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVRVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(3047..3766)
                     /locus_tag="OO161_003590"
     CDS             complement(3047..3766)
                     /locus_tag="OO161_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5464510.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3891..4454)
                     /locus_tag="OO161_003591"
                     /pseudo
     CDS             complement(3891..4454)
                     /locus_tag="OO161_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4438..4696
                     /locus_tag="OO161_003592"
                     /pseudo
     CDS             4438..4696
                     /locus_tag="OO161_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4805..5383)
                     /locus_tag="OO161_003593"
     CDS             complement(4805..5383)
                     /locus_tag="OO161_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464511.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISEHTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(5804..6041)
                     /locus_tag="OO161_003594"
                     /pseudo
     CDS             complement(5804..6041)
                     /locus_tag="OO161_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1741 a         1387 c         1150 g         2018 t
ORIGIN      
        1 cttacgcagt tgcataactt cagattccag ctcagccact gtgcgggagc ctggtgtatt
       61 gagccctttt cttgcggcgg taacccattg tcctaaagtg ccttcaggaa cagataatcg
      121 ggaagcgcct tcactgatcg aaagctgatt ttcaggaacc gttttgacgg cttcggtttt
      181 aaactccgta gagtaacgtt gggtttttct gctcattatt ggctccttct gatgccattc
      241 tatttcagga aggagtgtcc gttaaactca ggctacctca gataagcttg atacaactgg
      301 tctggtatat acagtttctt tgagtgcggt tatgtctaca gaggtaacgg taaaagtaac
      361 tccttgtcgg gaccactccg accgcgcaca gaggcatttt catcgctgac aggccgcatt
      421 gcctcgcagg gctggaggag gatcggtgat atccggacga ccgtaccggc ttacgccggg
      481 cttcggcggt tttactcctc tgccatgtgc aacaacggag tgccgcctta catgccgctg
      541 gcgcggcata tcctggtaac atttttgttg ataccacctt ccggccttat cctgaccagt
      601 gggctttcct ggcatcgatg ataccgatga acgcgcatga tattgagccg acgatattac
      661 gggcaactgg aaatactcac cctctggatg tgacctttat ccatgaagaa gatttggcca
      721 taccatggaa acctgaacaa tcatcagttt acgcccatgt aaacccctac gggatatttg
      781 aactcgatat ggaaacaagg ttgccaatcg aggtggttgc ctgaaagttg gcagttaccc
      841 ggtcactaca ccttgttgga gtgaacatat caaaaacagg taattataca atttaaatta
      901 cagtctcata aaaaatgatt aaatctattt attatttcaa taaacgtttt tttcatcttc
      961 tttgaaaatt gttctgctat agtatccggt tccagattat tctcttttat tattctacaa
     1021 tactctaata ttgtttcttt gtctctattt actacagata caaatacacc aggaatacca
     1081 tctttattag ttgcggacag catttcgacc aatcgtcttg tggtaaaact atagtttttt
     1141 agaatattac aaaaggcacg tattgtttcg gtatggccat tataaaatgc cataaaaagt
     1201 cctgatgtac cgttgggatt ttttatccat aataattcat ctatatattc ctggtgaggg
     1261 gggagtattt ttagtatatc tccatacgcc ataatggcct ttgtgtgccc attttgaagt
     1321 gccataaaca acccatgctt tactttcaat aattctttaa tttgttcact actaagcatt
     1381 ttcttattaa gcagacttcc gaacatacga atactatctg catgtccatt ctgcaatgca
     1441 aaaaataacc caggtaaatc atggctgtct ttcgcagcta atagatataa tctcttatcg
     1501 gaagttaacg gggttttaaa gactatttcc ccataggctt ttattgtgtc tacatgtccg
     1561 ttcataaaag acatgcataa acccggcaca ttgtttgccc ctaccgcagc aagcaatgta
     1621 agtattgttt cttgactaag attgcacata tttaatatct taccatatac ttgaataatg
     1681 tctgaacatc cgttttgaag tgctatataa aatgcaggag taccatcaga gcttctcgtg
     1741 gccagaaggt tcaccagttt agattgagaa atttcaccat ttttgtattt ttccatcagt
     1801 ataaataata cttctgtcag attctttgtt atcgcatgat atataactct ggaattgatc
     1861 aattcatctg gtaaaagttt cactgtgttt gttagagggg aattatgcct atcttcaaat
     1921 attacctgcc cgggttgctc cagaccataa attttccaat aaaaatcatc aaaaaaatcc
     1981 ttagcggata attgactcag ttcgaaatgg ctatcacaag taaatacagt acgtttatga
     2041 gtaacagttc gattaggatc gaaaaactga acaacccatt tttcactttc ttgtacgcgc
     2101 tttattttca accctaacgc catttggtgg tttaatgtgc atgctgataa tgttctgaca
     2161 aagtctccat tttttttcat gttataaaaa aaattagtaa taactgttcc ccacttttta
     2221 ttttccacaa aataaatata agctggtgat tttgaaaaat cttccgtttt ttccaaacat
     2281 acattttttg caatagcatc aggggtgcta agaagctgat agtttacctt cccatttgtt
     2341 tccataaaat tcatttccca ataggaagct aggtgacggc aaactatttg tcttccatat
     2401 atatcttttg ccactccatt tatatttacc ctcacatttc tcttttctga aaaataaggt
     2461 gctgttttat tcttaaactg aattagttca ctggagtttg agaccctcat aatagaactt
     2521 gttattgttc tattatctct gttaacagac atatctgttg ataacgaatt ttttgttgaa
     2581 aatatactag atgaaaatgt tttatttaac ggcatagaag acaaccatag agatgttata
     2641 atactattag attgcagtta ctaatagtaa aagcctcctt acaaggaggt agcttattca
     2701 gaatgctcct ataagatagc gagctcgtca acgatacaga tgatctgatt atattgtgca
     2761 aaaggagaac aggaaaatag gcactggaaa agatgaaggc ctgatgggag cattaactaa
     2821 tgcaaaatcc gacaggttaa tatcccgaaa agcaatcaaa cgactcaaac aggagatgca
     2881 cgctgaagtg gtggacaact gttcggattt cagatgagta gctcagcagt ttatgagccg
     2941 aatgctctgt cacggtcagg aatcaggctg catacgctgt gccaaatcta atcgtagtta
     3001 ttctaaccag ttttcaagct acaaggtggt gtagctggca tcttctctac tctatcatca
     3061 aacgataaaa tggttcctct cgtaacattt gctcttgcct ttcacttcca tctcgatcgc
     3121 ttcgtagttc attacgatag ctgacatatt tccagtcttc aggtctaaca tctgacgccg
     3181 gatactcccc gggagcgatt ttgcttctta atagattaga ctcgagacac attataaatt
     3241 gtcgtatttt atgaagtaat aaagcactat attgcgagca ttcctgatct gattttacat
     3301 atagcgtaaa ctgagctcct atccccacac gagattgctg agaaactcga ttcatatcag
     3361 ttatcttcca tttatctata ggactgtcct ctgaaaataa taaaccagac aaaatttgga
     3421 atgccagagg aacttgctcc ctagctatac taatatgaaa cttgtcccca acaaaatccc
     3481 ccttactctg aggattttca cgacatgcat gaataaacac atctccacta tggttcacaa
     3541 tctggaatcc ttggtacatg acagaaaaaa attgacatat atcagagtaa gttggcacgt
     3601 tcgatgctac tttgaaatag tacgctgggt attgccgtat ttggttgtac aatatattga
     3661 agttattttt caatctttca ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat
     3721 tcaaagaatc taaatttagt tttagacagg gtttttttat gggcatagaa aacgtcctct
     3781 ataaagtaga tatattatct tatattgatt gtttatcatc ttttataaaa cgatacatta
     3841 tatgtaatgt atgtaagcgt aagggtcttc tccgatcatg gtgggaaggc ttagaacgcc
     3901 atattcagtt tcccatagtg gaacatcact cccagcttaa agcgctcccg gttgcggtag
     3961 cccttggctt ttattctcag cagcctgatc ttgctgttca gtgcttccgc atttccgttt
     4021 gatacgccat ggcgcatggc gttcaggata ccgtacaacc ttttccctat cgttttagcc
     4081 acattcttca tcatggggac gtcactgttg gccgccagcg acagccatct ctgccagtcg
     4141 ttgcgtcttt cctcgctcca tggtctgctc cagatatcct ttgccagttc tttcagcgcc
     4201 cagcacaggc tcgtcagctt catctgcgca cgcagccata tcagcttttc ctgcctggat
     4261 tcggtcatcc atttatcgct gtgctgccac aggaagcgag ttccctttgc ctgacgacga
     4321 ctttcaacag gaagatgcgg atgttcattc tggcgggttt tatccaccac ctcacccggt
     4381 tgtttcgcca catgaaagcg atcgaaggct attttctcaa ccgcattggg taagtggatg
     4441 cgcgctgctc ttagtttgct gagccagttt agcattgcct tctacatgac cgatgcctga
     4501 gccggtatac cgaactttat tgtcctcaac cagtcatgtt ataagcaaga aatgcactca
     4561 gcggatagag cgacataatc tgaacctgcg gacacatctt aagcggctta cccgcaaaac
     4621 catatgcttc tcaaagtctg atgatatgca ctacaagatc atcggctggt atctgacaat
     4681 caaccaccac cactaaatct gcgtcacgac ctgcaactga gtctggttga acaggctgag
     4741 gctctgctga agcaacgcga acctctcgac tcttgttgga ttttaccaaa tagtttttct
     4801 aaaattaaac acatttatat aagttctttg ctgaacaaaa cgcagcccgc catccttttt
     4861 cattcataaa ttcagcaatg gcagataaaa ctttttcatc aagcatataa aaaataatgc
     4921 aagtagactc aatatgcctc atacaaataa ggccgcacat atccgtaata tatttgtcaa
     4981 tgctgctata atttgcatgg taatatgcca tgctattatc tgatgatttt acatatgcag
     5041 tatgacatat ttttccttca cgattaacaa aactgacaaa accactttca ccaggagtaa
     5101 tatataaaga tgaatttaac gtgtgctcac taatattcat taattgttga aattcaatgg
     5161 aatagttttc agttaatgta tctattatct tggctttgtc tttctttgtt atctcattgt
     5221 ctttttcggc aagaaaaatt gccactttat gacatgcatt cagctcgtta attttacctg
     5281 ataaaactgc ttttcttacc acatcgttga actcatcgat agtgtgtttt ttattatata
     5341 tatcatgaaa aatatagcct aaagacttaa acgagacttt cattttatat tctccttaaa
     5401 tattaacagg atatatatat cacattttcc tctatatttt cagaaaccag ctatcatttt
     5461 ccccattact ggtacgtcgc ttcagatatt tccgcagcaa gcgataaccc tgcgcactat
     5521 gaggatgcca ttcattataa tgtttgaaca ccttcgcgag actactttac tgctgttaac
     5581 acgtcaggtt cgaacataac actgatttaa tcatatttca tcgttttgac gaagttttct
     5641 gccattccat tttgcaccgc cgtgtgtttg ggggcaaggc cgaccatctg tgtttcattc
     5701 gccagatata agtgatgaga tggcattctc cctccttcac ggcagaagca ttttgaccgt
     5761 ttaggggatg cggacgattt tcatgcctgc taggatactt tcgttactga tgctgtccgt
     5821 tttccaccat ttcatcatca ctgattggcg ttgtgttttt ggtcagcact ccagctttac
     5881 gtttctcttt taaccggtaa ctctctcctt tgatattcag cgtgtagcag ccgatctagg
     5941 atcgctgttg ccagcacgtg atctccgaac atttctcccc agtctgcgac ccctttgttt
     6001 gacgtcagta tgatgctcgc tttttcatat cgacagttca tcgataggaa tttaaatccc
     6061 caaaagatta aaaaagcacc gaagaatgcg cgtttattca acacaacctg atcatcaagt
     6121 aatcggtata gacacttaat attgcttaca ttgattgatt caatgtatct ctgatggttt
     6181 tttgctccgt tcccctcaac atggacgcaa cttcccctct gcgactctca gccgcaccac
     6241 cgtattcgag ccagcggctc atgcatcata acctgctctg ccagacggta acccct
//
LOCUS       DAJTNA010000205         6264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTNA010000205 DAJTNA010000000
VERSION     DAJTNA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.208"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO161_003595"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO161_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5464512.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO161_003596"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO161_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR5464513.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            835..1995
                     /gene="pheA"
                     /locus_tag="OO161_003597"
     CDS             835..1995
                     /gene="pheA"
                     /locus_tag="OO161_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5464514.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2038..3159)
                     /gene="tyrA"
                     /locus_tag="OO161_003598"
     CDS             complement(2038..3159)
                     /gene="tyrA"
                     /locus_tag="OO161_003598"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5464515.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3170..4240)
                     /gene="aroF"
                     /locus_tag="OO161_003599"
     CDS             complement(3170..4240)
                     /gene="aroF"
                     /locus_tag="OO161_003599"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5464516.1"
                     /translation="MQKDAQNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..4813
                     /locus_tag="OO161_003600"
     CDS             4448..4813
                     /locus_tag="OO161_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5464517.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4963..5481
                     /locus_tag="OO161_003601"
                     /pseudo
     CDS             4963..5481
                     /locus_tag="OO161_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
     gene            5471..6264
                     /gene="dgcN"
                     /locus_tag="OO161_003602"
                     /pseudo
     CDS             5471..6264
                     /gene="dgcN"
                     /locus_tag="OO161_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
BASE COUNT         1699 a         1598 c         1410 g         1557 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat tcccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacactgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacacac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaaccgggg ggcctttttt attgataaca aaaaaggcaa cactatgaca
      841 tcggaaaatc cattactggc gctgcgagag aaaatcagcg cgctggatga aaaattatta
      901 gcattactgg cagagcggcg cgaactggcc gtcgaggtgg gaaaagccaa actgctctcg
      961 catcgcccgg tacgagatat tgatcgtgaa cgcgatttac tggaaagatt aattacgctc
     1021 ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct catcattgaa
     1081 gattccgtat taactcagca ggctttgctc cagcaacatc tcaataaaat taatccgcac
     1141 tcagcacgca tcgcttttct cggccccaaa ggttcttatt cccatcttgc ggcgcgccag
     1201 tatgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt tgccgatatt
     1261 tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg taccgattga aaataccagc
     1321 tccggtgcca taaacgacgt ttacgatctg ctgcaacata ccagcttgtc gattgttggc
     1381 gagatgacgt taactatcga ccattgtttg ttggtctccg gcacgactga tttatccacc
     1441 attaatacgg tctacagcca tccgcagcca ttccagcaat gcagcaaatt ccttaatcgt
     1501 tatcctcact ggaagattga atataccgaa agtacgtctg cggcaatgga aaaggttgca
     1561 caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac tttgtacggt
     1621 ttgcaggtac tggagcgtat tgaagcgaat cagcaacaaa acttcacccg atttgtggtg
     1681 ttggcacgta aagccattaa cgtgtctgac caggttccgg cgaaaacgac gttgttaatg
     1741 gcgaccgggc aacaagccgg tgcgctggtt gaagcgttgc tggtgctgcg caaccacaat
     1801 ctgattatga cccgtctgga atcacgcccg attcacggta atccatggga agagatgttt
     1861 tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt gaaagagtta
     1921 ggggaaatta cccgttcaat gaaggtattg ggctgttacc caagtgagaa cgtagtgcct
     1981 gttgatccaa cctgatgaaa aggtgccgga tgatgtgaat catccggcac tggattatta
     2041 ctggcggttg tcattcgcct gacgcaataa cacgcggctt tcactctgaa aacgctgtgc
     2101 gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct gcttatcgcc
     2161 ctgctccagc aactcaatcg cctcgccgaa acgcttatag taacgtttga ttaacgccag
     2221 attacgctct gacgacataa taatgtcggc ataaagctgc ggatcctgag caaacagtcg
     2281 cccgaccatc gccagctcaa ggcggtaaat cggcgaagag agcgccagaa gttgctcaag
     2341 ctgaacattt tcttctgcca gatgcagccc ataagcaaaa gtagcaaagt ggcgcagagc
     2401 ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca gccgagcgcc
     2461 ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc catcacacca
     2521 gaccacaact tgctttgcca ggctaccgct gtccgggccg aacatcgggt gtaaccccag
     2581 taccgggcca tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca ctgatgccag
     2641 atcaaccaga atacaatctt tcggtaaagg cggtaatttg ccgataactt gctcagtaac
     2701 gtggattggc acactaacaa tcaccattcc ggcatcggca acaatatcag ccgctcgatc
     2761 ccagtcatgt tgctccagaa tccgcacctg ataacccgag agtgtcagca tcttctcgaa
     2821 caggcgtccc atctgaccgc caccgccgac gataaccacc ggacgcagtg acggacaaag
     2881 tgttttaaat cctttgtcgt tttcactgga gtaagattca cgcatcaccc gacgcaaaac
     2941 atcctcaatc agatctggcg gtacacccag agcttccgcc tctgcacgac gcgaggccaa
     3001 catagatgcc tcgcgctccg gaacataaat aggcagtcca aagcggcttt tcacctcgcc
     3061 cacttcagca accagttcca gacgcttcgc taataaattc agcagcgctt tatcgacttc
     3121 atcaatttga tcgcgtaatg cggtcaattc agcaaccata ataaacctct taagccacgc
     3181 gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca tcggtcattt
     3241 cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg cgcggttgct
     3301 cagaagactg attgccctcg tggatattac tttcgatcat cagaccaata attgagcgat
     3361 tgccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg cgataatctt
     3421 tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc gcctgttcca
     3481 tctctttttc acattgcgca acatccgcag ggctatagtt cggcgcttta ccaccgcgca
     3541 ggatgacatg cccgtccgga ttcccctgag tttgtagcaa cgcaacctgc cctgcctggt
     3601 taatgccaac aaaacggtgc ggctgggcgg cggcgcgcat agcgttaatt gctgttgcca
     3661 gactgccgtc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag gccatttcac
     3721 ggtgagtttg cgattccgtt gtacgagcac caattgctga ccagctaaac agatcgccca
     3781 ggtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc atattcacca
     3841 gctcaagcag caatttacgc gcgatctgca gcccggcttc tacatcaaaa gagccatcca
     3901 tatggggatc gttaattaac cctttccagc cgacagtggt acggggtttt tcaaaataga
     3961 cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta aatcgacgag
     4021 catattccag agcagtttcc ggatcatgaa tggaacaagg accacaaact accagcagac
     4081 gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca atctgggctt
     4141 cttgttgcag gctcaatgga aaagcggcct tcagttgttc cggagtcatt aaaacctgtt
     4201 cgtcggtaat atgtacgtta ttctgcgcgt ctttttgcat gatggcgatc ctgtttatgc
     4261 tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata aagtaaagtt
     4321 ttcaatccat atttcgtacg cttttcttta cacgaacaat ttactcgcgc tttgatgctt
     4381 atttcgtaca aataaattta cacctccttt aaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttattggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcgggca gcgccataaa agatgacgat gcatttggcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaaggacaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgta acaatgttga
     4741 aagtgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
     4801 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4861 ttatttctca cattgatgac gatcgcatga aaaaatgata ttttaatgag cattctgaaa
     4921 aaatatcgat gcatttcgag cgaagaaggt ggggatccct gaatgcgttt ttctcactga
     4981 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca ggagccttct
     5041 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     5101 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     5161 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     5221 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     5281 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag
     5341 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     5401 aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5461 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5521 attacgcaac atcagtatga ccagcatatt tatcactatg atgctgatct ggttgctgct
     5581 ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc
     5641 agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac
     5701 tgaaacactg gcagcgctgg gccagcaagg gcaattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcgc ctggcattac acccgtaagg atccaggcga tactttcagc
     5821 aatttcataa gccactggct cttccccgcc cccatcattc agccgattcg tcacaatggt
     5881 gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt caatcagcca ttttatctgg
     5941 ttttcgctcg ccgtactgac cggttgtatt ctgctggcat caggcatcgc aattaccctc
     6001 acccgccatt tgcacaatgg cctggtggaa gcgctgaaaa atatcaccga tgtcgtacat
     6061 gatgtgcgtt ccaaccgcaa tttttcccga cgagtttctg aagaacgtat cgctgagttt
     6121 caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg gcagcttcgt
     6181 ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt aaccgcgcgc
     6241 agcgtttcgt agcggcatca atac
//
LOCUS       DAJTNA010000206         6228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTNA010000206 DAJTNA010000000
VERSION     DAJTNA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.209"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(110..1501)
                     /gene="tcyP"
                     /locus_tag="OO161_003603"
     CDS             complement(110..1501)
                     /gene="tcyP"
                     /locus_tag="OO161_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR5464518.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLVLMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLTAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1634..2224)
                     /locus_tag="OO161_003604"
     CDS             complement(1634..2224)
                     /locus_tag="OO161_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5464519.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKHQVEY"
     gene            complement(2387..3055)
                     /gene="hxpB"
                     /locus_tag="OO161_003605"
     CDS             complement(2387..3055)
                     /gene="hxpB"
                     /locus_tag="OO161_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5464520.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3202..3738
                     /locus_tag="OO161_003606"
     CDS             3202..3738
                     /locus_tag="OO161_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5464521.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3779..4639)
                     /locus_tag="OO161_003607"
     CDS             complement(3779..4639)
                     /locus_tag="OO161_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5464522.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4745..5035)
                     /gene="ghoS"
                     /locus_tag="OO161_003608"
     CDS             complement(4745..5035)
                     /gene="ghoS"
                     /locus_tag="OO161_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5464523.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5136..6065)
                     /gene="pfkB"
                     /locus_tag="OO161_003609"
     CDS             complement(5136..6065)
                     /gene="pfkB"
                     /locus_tag="OO161_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5464524.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1622 a         1557 c         1522 g         1527 t
ORIGIN      
        1 tttcgctgac gatctgttta aatatgagtc agtatgcatc gccgcgatgg aacatttaaa
       61 aagtaaagca acggccccta tacaaaacgg accgttgcca gcataagaat taacggtgtg
      121 ccagttcggc gtcgtcttcg ctatccagaa tggctttatc ggtttgcttc agccactggc
      181 tggtcagcgt gccagctgtc atcgagccac taacgtttag cgccgtacgg cccatgtcga
      241 taagcggttc aacggagatt aacagcgcca ccagggttac tggcaggccc atcgcagtca
      301 gcacaatcag tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct gcggaactaa
      361 cggtaacaat accgaccagc gtcgcaatcc acatcgggtc cagcgggtta atgccaaccg
      421 taggcgcaac catcaccgcc agcattgccg gatacaaacc ggcgcagccg ttctgaccaa
      481 tggttgcacc gaaagaggcg gcgaaactgg cgatggattc aggaacgccc agacgacgcg
      541 tttgtgcttc cacattcagt gggatagacg cagcactgga acggctggta aaggcaaacg
      601 tcagcacagg ccatacctta cggaagtact tcagcggact cacgccatta atgcccagca
      661 gaatgccatg cactgcaaac ataatcagca gaccgaggta ggacgcgaca acgaaacttc
      721 ccagtttgat gatgtcttgc aagttagaac ctgcaaccac tttggtcatt agaaccagaa
      781 cgccgtaagg ggtcaactgc atgacaaggc gaaccagttt catcacccag ctttgtaggg
      841 tatcgatagc ggctaagacg cgttcacctt tcggcgcatc atccttcagc agtttcaatg
      901 cagctacgcc gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc ggattggctc
      961 cggtaagatc ggcaaacggg tttttcggga taaaggacaa gaccagctgc ggaacgctca
     1021 gatcagagac tttaccaaca tagttacttt caatggcgtt cagacgtgca gtttctgcac
     1081 caccctgaac cagaccttca gccgtcaaac caaacaggtt ggtgaccagc acaccgacca
     1141 gcgccgcaat cagcgtggta aacaaaagcg taccgatggt cagaaaactg attttgccta
     1201 actgagatgc gttatgcaga cgggcaaccg cgctcagaat agaggcgaag actaacggca
     1261 taacgatcat ttgcagcagt tgaacatagc cgttaccaac gatgttaaac cactgtacag
     1321 aatctttaag tacctggctg tcagaaccat aaatggtatg cagggcaagg ccaaaaacca
     1381 cacccatcac cagacccacc agcacttttt tcgccagact ccactgttta tggcgggtct
     1441 gagccagcgc aaacagcagt acaacgaaca ccacgatgtt cgcaattaat ggaaagttca
     1501 tccccgttct cctgatctta ttatttctat cactgcgtag cagtcatagt gttggcgcaa
     1561 gattaacaga actgtgagct ggctcttata ttcaaatgga attgcttatt ccagaaaggt
     1621 gatttttgtc tttttagtat tcaacctgat gcttaataag ccgatgaaac tggatctgca
     1681 aggtattgat tatttgcgat gaccaacgaa cagcgctgtt ctcgggtaat gaatggggca
     1741 tccataccga ccagacaaat aatgccatgc agataaaacg ttccagttgg ttgccctttt
     1801 ggggaactag gataggcaag cggaaacgcc agcggcatgg ccagagcagg ggaacaccgg
     1861 cgggtgtcag catatcggca agtatgtggc tcaaataacc cagcaccatt ccttgtagcg
     1921 catcagccgg aatgaaccag ccttccggaa ccttaaggta aaaggttgcc agcagcgcaa
     1981 ataccgccag cagactgtgg gtaaaaccac gatgcccaaa agcgcgggcg atcggttttg
     2041 atatccattt taatcgctgc ccaagaaacg actttgggtg atcgatgtcc ggtaacaaac
     2101 acgtcaggat tgcggaaggg acaatatgcc accagtcacc ctgtgccagc acgggcgtca
     2161 gctcggcatt tttggcaaat accgcacaag caatagaaaa gagaaggtga ccttccgccg
     2221 tcatgataaa actcactaaa ctgtcgatac gtacagtata ggatttttat acagtgcacg
     2281 gaagtggtga cggtgtaact ctttctctta aattgacgat taatccgtca gttgaatgca
     2341 aattcagata aagaaccagg ggcagcgtga tcactgcccc tggtcattaa ccgagaaggt
     2401 cttttgcggt gagttctgtc agcgatgaga gtttgacgtt tgctaataca aaacgtggat
     2461 cattttgcgc ttctggcgca ggaacgacga tggaacgcat gcgggctgct ttagaagcga
     2521 tcatgccatt taccgagtct tccagcgcca cgcaggtcag tgggtcaacg cccagttttg
     2581 ctgcgcagtc gagatatact tgcggatgcg gcttgctgta aggcagtttt tcagccgagg
     2641 cgagggcatc gaaactgtcg cgtaagtcaa acatggtcaa cactttttcc agcatatgta
     2701 gtggtgacgc ggaggccagt cccaccaata aaccttgttc tttgcataac gcaacagctt
     2761 cgcgtacgcc tggtaataat ggacgtgtct cttcaaccag tgaaatggca cgggcaataa
     2821 cccgttctac tacttcctga cggcttggcc cattccacgg ttgccgggcg taccaaagat
     2881 cgaccaccat atcgatgcgt aaacctaagg tgtccggcag ctcgttacga cgggagatat
     2941 ccacccccag gcttgccatc acatccagtt cggctcgatc ccataaaggt tctgagtcga
     3001 taagtaatcc atccatatca aaaattgcag caagaatctg acgcggggtt gacattacaa
     3061 cctctccttt agagcattat gtgtaaggta ttaagccgtg cttatgatta agcgcctatc
     3121 atatcgcctt taaagaatgg gcgaaaacag atatctacag gtaaactaag tgaaacgacg
     3181 cgtcttaatc aaggagaact catgacgtat caacaagctg gacgcattgc tgttttgaaa
     3241 aggattttgg gttgggtgat ttttatacct gcattgatct ctacattgat ttctttgctg
     3301 aaatttatga atacccggca ggaaaaccag gaaggcatta atgcggtcat gctcgacttt
     3361 actcatgtca tgatcgatat gatgcaggcg aatacgcctt ttttgaatct gttctggtat
     3421 aactccccga cgcccaattt taatggtggc gtgaacgtta tgttctgggt gattttcatc
     3481 ctgatttttg tcggactggc gttgcaggat tccggtgccc gaatgagtcg ccaggcgcgc
     3541 ttcttgcggg aaggtgttga agatcaactc attctggaaa aagccaaagg ggaagagggg
     3601 ctaacgcgtg aacaaattga gtcccgtatt gttgttccgc accatactat tttcctgcag
     3661 tttttctcac tgtatatcct gccggttatt tgtattgctg caggttatgt gttcttttct
     3721 ctgcttgggt ttatttaatc aaaaggccgc acagagcggc cattatcttc aacccatatc
     3781 atgctgctaa taatctatcc agtgactgct gagcaataac caaatgctga ccgccaaata
     3841 accttgcacg atttaacagc gtgtagagtt ggtaaaccgg ttgacgttca aggaaatcgg
     3901 caggtagcgg ggatactgac tgatagccgt catagatttg tggcggctgt tcagtatgca
     3961 gcggtaacat cgccaggtcg cactctcggt caccccagta gcaggcaggg tcgaaaatgt
     4021 acgggccatc cggacccagc gcacagttgc cggaccataa atcgccgtgc aacagagaag
     4081 gctgcggttg atgcgaggcc agacgctgct gaatatgctc gacgatggcg tcgatattgc
     4141 cgaaagcgat ccctttctct gctgccagtt ccaactgcca gccaatccgt tgttcagcaa
     4201 aaaacgttga ccagcgacgt tgccaggtgt tgggctgtgg agttgtggag agcgagttat
     4261 caaaatcgag gccaaattgt ggttggtcac tccattgatg taaacgcgca atttgctgac
     4321 caagaataaa tgcgctatgc gcatccagcg gacgaggtgg gagataatcc atcaccagaa
     4381 aactgtagtc acggtcagcg cctactgccc aaaccttagg cacggtgacg gttttactac
     4441 gcgacagtaa ctccagttgg tcggcttcgg cggtaaaacc gggaagcagt tccctttcat
     4501 cacatttgac gaaaaagtca tgtcctgcat agcgcaaatg ccatgcggca tggacttctc
     4561 cgccaggcag ttcattacgc agttcgattt cgccttcacc taactgctcg ctcaaaagac
     4621 gactgattgc ctgccacatg atgtctctcc catgttgtct gccagatcat aaggttagcg
     4681 caagaatgcg gtgtaaaaac acgagctaac gcacaccaca gactgattat tgcccaaaaa
     4741 ccatttattt tctgttgcta ttccattcct cccaggtcgt gatggtgatt tcaggatctt
     4801 tgccggttgc actttgggta agccccgaaa ccagttctct aatttcttct tcactttggg
     4861 tactgataag tccaaaagtg ttagtcccca gctcatggat atttccctca tcatccgtca
     4921 tggtgagcaa aaaaccgtcg cgagtcaggt aattattcaa ctcgttaatt tcagtcaatg
     4981 tatcctcatg caacatgacg gttatgacat aacggacaag atcgccactg gccatagttc
     5041 acccctttgt tatcacatga cttttgagca tagtcggaga aacgcgttgc cgacaggttg
     5101 gtggtgattc ccccaatgct gggggaatgt ttttgttagc gggaaaggta agcgtaaatt
     5161 ttttgcgtat cgtcatggga gcacagacgt gttccctgat tgagtgtggc tgcactcccc
     5221 gcagctacgc caaaacggac catctcttca agagaggcat tttctgccag tttcagtgtc
     5281 atcgcgccga ccatgctgtc accagcgcca acggtactct ggcttttcac cggtggtggc
     5341 accacctgaa tgcagttttc actatcaaca cccagcgctc cttgtggacc aagggaaacg
     5401 acaacccgtt tggccttgcc gctattaacg atttcctgcg cggctttgcg gacatcgtcc
     5461 ggctgggtga gttcgcgatt caccagcgca ctgagttctt tttggttagg cttaaccaac
     5521 tcgatgttac caattgccag tgctgcactt aacgcttcgc cagaactgtc gacgatgcag
     5581 cggatccctt gtttttgcga agcggaaatc agttgggtta atttttccag cttcacacct
     5641 ggcggcaggc ttccgcttat gactaggatg gccccggatt caatttccag aacttgctct
     5701 tcaagctggc gaaactcatc ttcatttaat gccgcgcctg gcataacaaa acgatactgc
     5761 tcaccgcttg cttccacatg tacatgtaaa ttctgccggg tccagtcttt ggcttctaca
     5821 gtagcgacgg ggacattttc atccgccaac agtgaaacca ggtgttcgcc ggtcgcgcca
     5881 cccgccggga agatcgctgt ggcactgcct ccaagatggg caatggcgcg ggcgacgttg
     5941 atgccgccgc ccccgggttc gaacaccggt gcggtacagc gcagttttcc ttcgggataa
     6001 atttgcgggg taattgttgc gctatcgaga gagggcgcaa gtgtcaacgt atagatacgt
     6061 accatcattt cctcctatag gctgatttca gtctggcacc gaatcagcgg aaagtgaaga
     6121 aattaacaat atgatttatt ggagctttat ttaaaatttt gcagataaat atatataaat
     6181 aaaaatctgt tttcgccaca tgcttattca aaaaatgaaa taagaata
//
LOCUS       DAJTNA010000207         6227 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTNA010000207 DAJTNA010000000
VERSION     DAJTNA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.210"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(152..802)
                     /gene="zinT"
                     /locus_tag="OO161_003610"
     CDS             complement(152..802)
                     /gene="zinT"
                     /locus_tag="OO161_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5464525.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1059..1694)
                     /gene="msrQ"
                     /locus_tag="OO161_003611"
     CDS             complement(1059..1694)
                     /gene="msrQ"
                     /locus_tag="OO161_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5464526.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWALNHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(1695..2699)
                     /gene="msrP"
                     /locus_tag="OO161_003612"
     CDS             complement(1695..2699)
                     /gene="msrP"
                     /locus_tag="OO161_003612"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR5464527.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGMKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2808..3221)
                     /gene="hiuH"
                     /locus_tag="OO161_003613"
     CDS             complement(2808..3221)
                     /gene="hiuH"
                     /locus_tag="OO161_003613"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5464528.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3354..4025
                     /gene="hprR"
                     /locus_tag="OO161_003614"
     CDS             3354..4025
                     /gene="hprR"
                     /locus_tag="OO161_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5464529.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4025..5383
                     /gene="hprS"
                     /locus_tag="OO161_003615"
     CDS             4025..5383
                     /gene="hprS"
                     /locus_tag="OO161_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5464530.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVTDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYINKHNVFRITLPQ
                     RN"
     gene            complement(5491..>6227)
                     /gene="hchA"
                     /locus_tag="OO161_003616"
     CDS             complement(5491..>6227)
                     /gene="hchA"
                     /locus_tag="OO161_003616"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5464531.1"
                     /translation="DYPKPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLY
                     HLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLN
                     ADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDN
                     PVNGYSICAFPDAADKQTPDIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKL
                     LTGDSPFAANALGKLAAQEMLAAYAG"
BASE COUNT         1726 a         1321 c         1448 g         1732 t
ORIGIN      
        1 taactcaatg cgggttagct tattaatata cagacacgtc atagtgatga gcgtgtctgt
       61 agctagcttg cgttcagtgg cgtttgtgga tggaccagtg gcagttatga tgacttacta
      121 aagcggcatc gaggcgttat catgagaata ctcaatgaga catcatttcc tcgaccactt
      181 cttcgctact caactgatat ggataatacg ttggccagtt ttccatttca tttaataaag
      241 attgctgtga atcattaccc ataaaaatgt gaaaatgaga tgattttcgt ggtgcaatta
      301 tatgatcgct aaattgtata tatttagggg ctttgctttc aggatcttta cattcgaata
      361 agtaacgaac gcctttcttg cctgatttat aggtgagtat tttgtatcca tcgtaatcat
      421 atttacagga tgttgtttca ttatttctat ggaattcaac aatgccgtcc tcaatgccaa
      481 tcatctcgat atctgttgca taacctttgt gataataatc tttaatttca gcaaatgttt
      541 tagttttatc tgcatccgct ttcttctgaa agacggggtc aagtttgcca ctttgcagta
      601 aaggataaac ggattgccag actccatccc agtcactgag cgttcggttt tgtacattgg
      661 catcatcaaa aacaccatta gcagcttttt gttcgacctc tgttaagggt ttgccgtgtg
      721 agtgatgacc atgcgaaaag gcaggagcgc taacaataaa gacacctaac gcaacagcca
      781 gtttgtgaag acgaatcgcc aaaacagttt cctccagagt cattgtatat gatgtgtaat
      841 gttatattgt aacatatggc aaaatgcaac aactcttagg agatgagagc atagttttct
      901 caggtgtact tatctatctt attgaaataa aatgaattat attgataatt tgaggttgat
      961 gacaaacgaa acataatctg atgtgatctg cgtaggccgg atagggcgct cgcgccgcat
     1021 ccggcaggga taacgcgtac tttgtcggca atatgaagtt atacagataa tttgttgtga
     1081 acctgcttgc gtaaccggtt aaaaagggaa cgcaacttct tataccgtaa ggctaaaagc
     1141 agtacagcca gcccagcgta gatgagcggc tgcggtgaaa taatcttcac tgaccacaga
     1201 taatgtattg gggccaggat cgcgacaaga tagacgaaat tatgcaactg ttgccagtgc
     1261 ttgccgagtt ttcgctgcat cgcctgggtt gaagtaaacg ctaaagcaag caaaataacc
     1321 cagctgataa ttcctaacgt taaataaggc cgggtaatta actccttgcc taataacgcc
     1381 agattattca cgcccagctc cagcaatgcg taactggtta aatgcagtgt cgcccaggca
     1441 aagcaccata agcctaacag gcggcgagtg cgtatcaata acggctgttt tgcgtagcgt
     1501 gccagaggtg tgattaacaa ggtcgccagc aaaaatttca gtgcagtgcg accggtaaaa
     1561 tgctgaatat ctttcaccgg atcggcaccc agtccaccgt gattgagcgc ccagaccagc
     1621 cagagaaacg gcaacaatcc ggcaagatgc aggctaactt tcagccatgt cacctgtttt
     1681 gctgtcagac gcattcagaa attctcccgc aaatccaggc cacgatacag cgatgccacc
     1741 tggtcggcgt aaccattaaa cagtagcgtt ggctggcgct gaacatcgag gatgccgcct
     1801 gaaccaataa atcgttcggt agcctgtgac cagcgcgggt gatcaacatg cggattaacg
     1861 ttggcgtaaa aaccgtattc gtcaggcgct gccagattcc aggtggttgg cggacgctcg
     1921 cgggtcagct taatactgac tatcgattta atccctttaa agccatattt ccacggcaca
     1981 atcagtcgta ccggcgcgcc attttgtggc ggtaacgcct tgccataaac acccacggtc
     2041 atcagtgtga gcggatgcat tgcttcgtcg agacgcaatc cttcgacata aggatatttc
     2101 atcccgccgc cgataaagcg gtcctgctgg cctggcattt gttccggtgc ataaattgtt
     2161 tcgaaagcga catacttcgc attgctggtg ggttcggcaa gcgccagcaa tttgtgcagc
     2221 ggaaaaccaa tccacggcac caccatcgac catgcctcca cgcagcgcat acgataaata
     2281 cgctcttcca gcgggaagcg acgggttaaa tcatcgtgat cgagggtcaa tggttttgcc
     2341 acttcgccgc tgattttcag tgtccatgga tcggttttca ggctaccagc attagcggcg
     2401 ggatcggctt tatccagccc gaattcatag aagttgttat aaccggagac tttatctact
     2461 ggcgtcagtg gcaggttatt ttgccaggcg gcaggcttgc tgaactccag cggttttccg
     2521 gcgggggcgg gcgggcgatc gttcccttta aaccagctaa gcagatcggc atgcgcagcg
     2581 tgaggcaaag aaagtgcagc tgcactgatg cccagtgctt ttaacacctg ccgacgcttc
     2641 ataaagaata ccgactcggc cgtaacatct gattctttta aaaattgatt ctttttcata
     2701 agccatcaca cttttttgtc gttagccagc aagagccgtt tactgttaat ctgtcaggga
     2761 gtgtcctccc taacagtagc agagatctgc ttttacatat ttatgcatta actgccacga
     2821 taggttgaat acccatattg gctaagtaat aaaggcacat gataatgctc gttcacttta
     2881 ttaatatgaa attcaacggg gatctcaggg aagaaacttt caagattttg tttcttgaaa
     2941 tagtccccgg ttttaaatac gacacggtaa tcgcccgtag ttgcagtttg ctcgggccac
     3001 agtgccttaa ttcgtccatc cttatctgtt ttggcggtat taagttgtaa ccagccgtta
     3061 tccgccttct tttcaagagt gactgtcacg tcagcagcgg gttttccggt ttgctggttc
     3121 aaaatgtgca cgctaagaat gttttgttgt gcggcataaa ccaaagaagg taatgaaaat
     3181 gctgccgttg ctaccgagag tactaaataa cgctttaaca tgtttatatc cttgtcatgt
     3241 gaatgagttt tgaggatagc gaaaaagcgg ccagggtaac cttacagcag cattacaatt
     3301 ttgtaatgaa ccaggctgtt tctataacat atgatttatg gcatattatt ttcatgaaga
     3361 ttttacttat tgaagataat caaaggaccc aggagtgggt aacgcagggg ctttccgaag
     3421 cgggttatgt cattgatgcc gtttctgatg gcagagatgg gctttatctt gcgctgaagg
     3481 atgattatgc attgataatt ctggatatta tgcttccggg tatggatggc tggcagatct
     3541 tacaaacgtt aagaacagca aagcaaaccc ctgttatttg ccttactgca agggattctg
     3601 tcgatgacag agtcagaggg ctggacagtg gagcaaatga ttatctggta aaaccttttt
     3661 cattttctga gttgctggca agggttcggg cacaattaag gcaacatcac gctttgaatt
     3721 caacattaga aatcagtggc ttaagaatgg actctgtaag tcaaagtgtg agcagggaca
     3781 atatcagtat tacactgacg cgcaaggagt ttcagttact ttggctactg gcctccagag
     3841 ctggcgaaat tatacccaga acggttattg cgagtgaaat ttggggaatc aactttgata
     3901 gtgataccaa tacggtggat gtcgccattc gcaggctccg cgcaaaagtt gatgatcctt
     3961 ttcctgaaaa gctaatcgcc acaatccggg ggatgggcta ttcattcgta gcggtaaaaa
     4021 aataatgaaa agattgtcta taaccgtccg tttaaccttg ctttttatat tgctgctgtc
     4081 tgttgctggc gctggaattg tctggactct ctataatggc ctggcaagtg agttgaaatg
     4141 gcgcgatgat acaacactca ttaaccggac agcacagatc aagcagttgt taattgatgg
     4201 ggtaaatcca gatacgttac ctgtatactt taaccggatg atggatgtta gtcaggatat
     4261 cttgatcatt catggtgatg gcatcaataa aattgttaac cggacaaatg tcagtgatga
     4321 catgttaaat aacatacctg ctagtgagac aatcagcgca gctggcattt acagaagcat
     4381 tattaatgat actgagattg atgctttacg aattaatatt gatgaagttt cgccatcatt
     4441 aacggttact gtggctaaat tggcttcagc tagacataac atgcttgaac agtataaaat
     4501 taatagcatt ataatttgca ttgtcgccat tgtactttgc tcagtattaa gtccgctgtt
     4561 aatcagaacg ggattacgag agatcaaaaa gttgagtggt gtaacggaag cgctgaatta
     4621 taacgatagc cgagagcctg ttgaggttag cgcattaccg agagaactaa aacctcttgg
     4681 gcaggcgttg aataaaatgc atcaagcctt agtcacagat tttgaacgcc taagtcaatt
     4741 tgctgatgat ctcgctcatg aacttagaac gcccattaat gcattactgg gtcagaatca
     4801 ggttacgctc agtcaaacca gaagtatcgc tgaatatcaa aaaacaattg ccggtaacat
     4861 tgaagagttg gaaaatattt cgcggttaac agagaacata ctgtttcttg cccgggcaga
     4921 taaaaacaat gttttggtga aactggactc gctttctctc aataaggaag tcgaaaattt
     4981 gttggattat cttgaatatc tttcagacga gaaagagatt tgctttaagg tcgagtgcaa
     5041 tcagcaaatc tttgcggata aaattttact gcaacgaatg ttatcgaatc ttattgttaa
     5101 tgccattaga tattcaccag aaaaatcgcg tattcatata accagttttc ttgataccaa
     5161 cggctatctt aatattgata tcgccagtcc tggaacgaaa atacatgagc ctgaaaaact
     5221 cttccgtaga ttttggcggg gagataattc gcgtcattcc gtaggtcagg gacttggtct
     5281 ttctttagtc aaagcgattg ccgaattgca tgggggaagt gcttcatatc actatatcaa
     5341 taagcataat gtgttccgaa ttacgttacc gcaaagaaat taacataggg cttcagtacg
     5401 ccataaatta accgtgaaaa aacgcagcca cgtcgtgagt actaacgcgg ctgcgattaa
     5461 ttatgcgctt acattcaaac gtaacaggga ttaacccgcg taagctgcca gcatttcctg
     5521 cgccgccagt ttacccaacg cattcgctgc gaaaggactg tcgccggtga gaagtttacg
     5581 gtccttatgt actcgcccgg tgatgtcgtc attaatgatg ttcatgccca ttttcttcag
     5641 ctcttcgccg aagtaccagg tgagatgacc tggcatatag ccaatatctg gcgtttgttt
     5701 gtctgcggcg tctgggaatg cgcaaatgga ataaccattc actgggttat cgccgtggcg
     5761 aagcgccaga aaagccgccg ggccgtggca aagggagata acaaaacggt catttttgat
     5821 tgcccactgt aaagcggcag ctacgtcctg gctttcaggt aaaccaataa gtgcgccatg
     5881 accaccagga acaaagattg ctgcatattc gctatcagcg ttgaggctgg cgacaacatc
     5941 cgcgagtttc ttcggattgc ggaacaacga tttatgctgc tcaaagaatg gcattacttt
     6001 ttcatctttg tgcggcatag cccagtattc aaacttggtc atcagaccgg aaatggtcgc
     6061 cacttcgaat tcgaaacctg cagcatggag atgatacaac ggcagcaacg tttcaatcgg
     6121 atggttaccg gtcgagaaca gttttccgtt atcggttggc aaataacgtt cgtccgcggc
     6181 gatcaccaga attttatgct taccgcgata cggttttgga tagtcca
//
LOCUS       DAJTNA010000208         6225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTNA010000208 DAJTNA010000000
VERSION     DAJTNA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.211"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(49..174)
                     /locus_tag="OO161_003617"
                     /pseudo
     CDS             complement(49..174)
                     /locus_tag="OO161_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
     gene            complement(479..649)
                     /locus_tag="OO161_003618"
     CDS             complement(479..649)
                     /locus_tag="OO161_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464532.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(764..1032)
                     /locus_tag="OO161_003619"
                     /pseudo
     CDS             complement(764..1032)
                     /locus_tag="OO161_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1089..1757
                     /locus_tag="OO161_003620"
     CDS             1089..1757
                     /locus_tag="OO161_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5464533.1"
                     /translation="MVKSMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHVGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1812..2396)
                     /locus_tag="OO161_003621"
     CDS             complement(1812..2396)
                     /locus_tag="OO161_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5464534.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDELFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKMFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(2396..5371)
                     /locus_tag="OO161_003622"
     CDS             complement(2396..5371)
                     /locus_tag="OO161_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5464535.1"
                     /translation="MAVLISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENPD
                     EAGRYTMDVEYGQYSVSLLVEGFPPSHAGIITVYEDSKPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDARTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAEAASTKASEASKSAAAAESSKSAAATSASAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNAASSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAADSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEAAVLASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYALYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKNTSSFDYGTKSTN
                     NTGAHTHSLSGSTGSAGDHTHGNGIRWPGGGGSALAFYDGGGFTYVQDSQYQVSPGTS
                     SRRSYYQRIQTQSAGAHTHSLSGTAASSGAHAHTVGIGAHTHSVAIGSHGHTITVNAA
                     GNAENTVKNIAFNYIVRLA"
     gene            complement(5436..6035)
                     /locus_tag="OO161_003623"
     CDS             complement(5436..6035)
                     /locus_tag="OO161_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5464536.1"
                     /translation="MRKLCAVILSAVVWLVAAGMPASAAEHQSTLSAGYLQTHTDMPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAKDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVTYSRVSTFSGDYLRVTDSKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTNSFIVGVGYKF"
     gene            complement(6103..6204)
                     /locus_tag="OO161_003624"
     CDS             complement(6103..6204)
                     /locus_tag="OO161_003624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="J protein"
                     /protein_id="HCR5464537.1"
                     /translation="MSAWLVNNWYPTASISDLLVVVMKKATTGITIS"
BASE COUNT         1417 a         1665 c         1461 g         1682 t
ORIGIN      
        1 ccatgtcctt attgaccccg tatattacgg ggtcgttttt gtgcggaatt aaaaacgata
       61 tcctgctgag aacataaaca cccacggatc gaggcgtacg ctatcatgtt gctgtgcacc
      121 gcccagctta taattggcgg tggtatcgat atccatgtac cagactggta ggtttcaaaa
      181 aacacaataa ccaattgtat ttgaagcaaa aaacaagact ttgagatgtt ttttttgtac
      241 ctttttttat gtttttaact gtctgatttt attgaaatct tttttagttt tgcagaagga
      301 attttttact tgattgtaac tttttcgttt ttttgggggc taattgacgc gcaatgctgg
      361 gcggggagcg cacagccaaa aaaaccgaaa ttgaccgcta attactgcca atcatcatcg
      421 ctctgattga gcgtaaatca ttcaggttat tcagttcttc cttctgctct cgctgacgtc
      481 aataaatccc atcattgcga tcaacctcat ccttaaccat tgatgcgagc agttcttcca
      541 gtttctgcgc tgacagcttt acctggtgat cttcagcatc ttcccgggct attgtggttc
      601 gcgcagtggc tgattttgct gacatcacca cagggggcag acggaccatt gaaccatggc
      661 ctccggaaca aataagggca aaaataacaa ccactatcga aagctcacaa actaaccgca
      721 gcacgttcct gcatacgacg tgtctgcggc ataatcccaa tgattactcc ctgacaggat
      781 ttgcaggcca ctcaatatcg gtgcagttga tgtatcaaca cgattcaaca atacccgata
      841 tttattccat gcctccagca acgatctttc ttcctccgtt gcgatttcca gatctacagc
      901 atcctgcagt ggcgcaatat actcactgaa ttcctggatg tagaactgtg tggtggcggt
      961 cttccagcca ttcggctcct gctgtatcga agcataccag gctatttcaa tatcgctatg
     1021 ctgcggcagc atttaacccc ttgtaattca tcgccataat tgatttaatt cacaaataaa
     1081 actataacat ggtgaaatca atgaaaaaaa acacagatga tggggctaaa atttacacac
     1141 cacttaccct aaagctttat gactggtggg ttttgggagt atcaaatcgg cttgcatggg
     1201 gatgtcctac aaaggaacac cttcttccac actttctgga acatgtaggt aacaaccatc
     1261 tggatattgg tgttggaact gggttttacc ttactcacgt acctgagagt agtctgatat
     1321 ctttaatgga tttgaacgaa gctagcctga acgcggcatc tacaagggct ggggaatcaa
     1381 aaattaaaca taaaattagc catgatgttt ttgaacctta tcccgcggcg ttacatggtc
     1441 aatttgattc catttccatg ttttaccttc ttcactgcct gcctggaaat atatctacaa
     1501 aaagctgtgt aatacgcaat gcggcgcagg ccttaactga cgatggaact ctatacggag
     1561 ccacaattct tggcgatgga gttgtgcaca atagcttcgg tcaaaaactg atgcgcattt
     1621 acaatcagaa aggcatcttt tcaaacacaa aagattccga agaaggctta acacatatac
     1681 tctcagagca tttcgagaat gttaaaacca aggttcaagg tactgtagta atgttttccg
     1741 cttcagggaa aaaatagcat ccaaccgcag cacgttcttg cttaagacgt gctgcggcat
     1801 aatcccaatg attactccct gacagggttc gtaggccact caatatcagg tgcagttgat
     1861 gtatcaacac ggttcagcaa cacccgatac tttttccagg cttccagcaa tgaggtttct
     1921 tcctccgttg caatttccag atctgcagca tcctgaagcg gcgcaatatg ctcactggct
     1981 acctgcatca ggttgttttt tgtttcttcc gcctcccgga tccggaacat tttttctgct
     2041 tccgtatcct tcacccaggc tgtgccgttc cacttctgaa actccccttc cggcgataac
     2101 caggtaacat tttccggtaa cggaccgagt tcagaaataa ataactcgtc ccctgacgct
     2161 acgtcataaa ccgttttacc ccgatggtct tcaacgagat gccacgatgc ctcatcactg
     2221 ttgaaaacag ccacaaagcc agcaggaata tctggtggtg caatatcggt actgtttgct
     2281 ggcagacctg tatgaggcgg aatatatgcg tcaccttcac caataaattc attagttccg
     2341 gccagcagat tataaatttt tatggtccgt gcttgttcac tcattctgaa tgccattatg
     2401 caagcctcac aatatagtta aatgcgatgt ttttgacggt gttttccgcg ttaccagcag
     2461 cgttaacggt gatggtgtgt ccatgtgaac caatcgcaac ggagtgcgta tgcgcaccaa
     2521 tacctacagt atgtgcatgt gcgccagaac ttgctgcagt accagacagc gagtgggtat
     2581 gagcacctgc tgactgtgtc tgaatacgtt gataatacga tctacgggaa gaagtccccg
     2641 ggcttacttg atactgtgaa tcctggacat aagtgaaccc accgccatca taaaatgcta
     2701 acgcagaacc gccgcctcct ggccaacgaa taccattacc atgagtatga tcaccggcag
     2761 accccgtaga gccactcaga ctgtgcgtat gcgccccggt gttattcgtg gatttggttc
     2821 cgtaatcaaa cgacgatgtg tttttcgtcc ccaaatccgt actggatgcg ctggcgctgt
     2881 gggtgtgcga tttaatgccg tcctgttcct gagacaatac ggcacgacca ctggcgggtt
     2941 tgcccttaat cgtccagcca cgcatatcag ggatcacgcc tgacggataa gcggctgcaa
     3001 gtttcgggta tgcagatttg tcaaaagtct gcccctgcat cagggcatag ccagacggaa
     3061 cggtatctga tggccacggg attggtgcgc caggcggata aaactgctct gatggcgtat
     3121 agagtgcata aactgtaccg tccgttaacc cttccggctt attagcagaa tatgctggtg
     3181 acgtatgaat cgtcacgctg gcattactgg tataatccca ttgaatattt acaccagtcg
     3241 cataatttcc gattgcaacg taaatatcgt aagtatcacc agatgtattg acccaggcaa
     3301 aatttgtaaa ccctgtcgat gtgcgctgcc ataaagcacc agtaatcccc ttcggattac
     3361 cattacctgc acgcaaaaca agttcagata tacctgcctg ttgaggtgac cccacgttaa
     3421 atcccgcacc accaatcaac gtaattgaaa caacagaact cgcctgtggc atggttaccg
     3481 ttgctaattt gaaccaacca gcaccaccgc tgaatgacat tgttgttgag ttaagcgtac
     3541 caatatcttt cggcgtcagt gttatatccg ctgaaagcgc cttaccattc accttacggg
     3601 cagaaggtac ccgaccattc gcattatcat tagctgcttt cactgctttc ggtgtcgcgg
     3661 caagcgtttc agatgcgctg ttggttgcac tactgagctg gacaattcct ttttgtgctg
     3721 tcgtagcgtc ctgagcagta tatttcccgt tagcaaggtc ataggctgcc tttaccgact
     3781 ttggcgttgc cgccagcatt tcagatgtgc tgttggtcgc gttgctaagc tgaactatcc
     3841 ctttctgtgc tgtcgttgca tcctgtgcgg tgtatttccc gttagccagc tcatacgcgg
     3901 ccttaacggc ttttggcgtt gccgccagtg actcggaagt gctgttagtc gcactgctga
     3961 gctgtactat cccctttttc gtcgtgctcg catcctcaag cgccacggcg gatgcaatat
     4021 cctctgcccg ttttgccgct gtctcagcgc gcgttgccgc ggattccgcc gtacttttgc
     4081 tctgagctgc cgccgtcgca ctgccagctg cctctgtcgc cttcgtggat gccgtcgtgg
     4141 cgctgccctt tgctgctgac gcctgtctgg tcgcctcatc ttttgaagca gacgcagatg
     4201 atgccgatga cgccgccgaa ctggcggacg atgcggcagc cgtttttgag gattctgcgc
     4261 tggtttccga cgctttcgcg ttcgtctcgg atgtcttcgc tgcggaagca gacctcgctg
     4321 ctgcgctggc cagtacagcg gcttcgccag ccttcgttgt ggctgttgaa gcagacgatg
     4381 cagcactttc tgccgatttt ccggcggcgg tggcactggc tgaggcctgc ccggcacttg
     4441 ttgacgcggc actggcagat aatgcagccg ctgtttttga gtctgccgca gctgaggcgc
     4501 tctgtcccgc tgccgtttca gaagacctgg cgttcgtctc ggacgttttt gccgccttcg
     4561 cagaatttgc tgccgccgtt gccgaggaag ctgcgctgct ggcgctcgag gctgcgttcg
     4621 tttctgatga ttttgccgcc tcttttgaag ccgacgcatc ccgtgctgaa gtggctgctt
     4681 ctgacgcttt cgtggtcgcg gtggatgcag aagtggctgc tgattgttgt gacgctgccg
     4741 cattcgtttc tgacgttttc gcggcactgg cactggtagc tgccgcgctt tttgaggact
     4801 ctgcagcagc agcacttttc gatgcttcac tggcctttgt tgatgccgct tctgcgctgg
     4861 aagacgctga ctgagccgac gacgcggcct gtccggctga cgtgctggct gcgcgtgctg
     4921 agcctgcagc atcagtcgca tgggttgccg cctcacgggc tgatgtgcgg gcatcgctgg
     4981 ctgacttctt cgcggctgcc gtgttctgtg ccactgcgga cgcgttacgc gccacctctt
     5041 ccaccatcag ttcaaaacgg cgcagtgcct ctggccggac atcatcctcc gtcattgcac
     5101 cgaggaaatc attcagtgtg cccggctttg agtcctcata tacggtgata atcccggcgt
     5161 gtgacggcgg gaatccctcc accaacagac tgacgctgta ctgaccatac tcgacgtcca
     5221 ttgtataacg cccggcttca tccggatttt ctgaggccac tgtgttcacc accaccgtgg
     5281 tgctgttgcg cctggccttt agctgaatgg tgcagttttg tatcggctta cctgcaccat
     5341 ctttcagtac acctgaaatc agtactgcca tattcccccc acaaaaaagc ccgcctgaac
     5401 cggcgggctg tcataacact gtgttacctg gctaatcaga atttatagcc gacaccaacg
     5461 atgaaactgt tggtacgcca gtcaccactg ccggagcctt cataagcaat atcaatggcc
     5521 acggattcgg tcgggttaaa ctgcacgcca gccccccacg ccagagacgt gttgctgtgg
     5581 cgaccgtcat cacttccggt cagcacatcg tgcgttttcc ccttgctgtc agttacacgg
     5641 agataatccc cggaaaaagt cgacacacga ctgtaagtca cacccgccat cgcatacgcg
     5701 ctgaaccatt cattcacgcg cacagacggc cccgccatca cgctgaacca gcggttacgc
     5761 acggaatctt catgccagcg ggtatcgctg taatgcgttt tttgctcatc tttggcattg
     5821 gcataactga atgacgtcac cagccccagc gtgtccgtaa actcataacg gtatttcacg
     5881 ttaatcccgt tcagatcatc gctgcctggc atatcagtat gggtctgaag atacccggcg
     5941 cttagtgtgg actgatgctc tgctgcgctc gctggcatac cagcggcaac cagccagact
     6001 actgcggaca gaataacagc acataattta cgcataatta cctctcgctt ttctgcaata
     6061 aaaaaggcac catttctggt gcccgtatct gggttataaa attcagctaa tcgtgatgcc
     6121 tgtagtggct ttcttcatca ccacaaccag caaatcgctg atacttgctg tgggatacca
     6181 gttatttacc agccatgctg acaccgaaaa ctccagtgtc atgtg
//
LOCUS       DAJTNA010000209         6200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTNA010000209 DAJTNA010000000
VERSION     DAJTNA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.212"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO161_003625"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO161_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5464538.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO161_003626"
     CDS             2089..2238
                     /locus_tag="OO161_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464539.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OO161_003627"
     CDS             complement(2983..3423)
                     /locus_tag="OO161_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5464540.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OO161_003628"
     CDS             3641..4030
                     /locus_tag="OO161_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464541.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OO161_003629"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OO161_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="OO161_003630"
     CDS             complement(4585..5421)
                     /locus_tag="OO161_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5464542.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSSAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..>6200)
                     /locus_tag="OO161_003631"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OO161_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5464543.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1433 a         1616 c         1642 g         1509 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg aggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJTNA010000210         6186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTNA010000210 DAJTNA010000000
VERSION     DAJTNA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.213"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            143..346
                     /locus_tag="OO161_003632"
     CDS             143..346
                     /locus_tag="OO161_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5464544.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            354..1286
                     /gene="aaeA"
                     /locus_tag="OO161_003633"
                     /pseudo
     CDS             354..1286
                     /gene="aaeA"
                     /locus_tag="OO161_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            1292..3259
                     /gene="aaeB"
                     /locus_tag="OO161_003634"
     CDS             1292..3259
                     /gene="aaeB"
                     /locus_tag="OO161_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5464545.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTRRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3351..3623
                     /locus_tag="OO161_003635"
     CDS             3351..3623
                     /locus_tag="OO161_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5464546.1"
                     /translation="MNIYTFDFDEIDSQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3679..3942)
                     /gene="yhcN"
                     /locus_tag="OO161_003636"
     CDS             complement(3679..3942)
                     /gene="yhcN"
                     /locus_tag="OO161_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5464547.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(4307..4777)
                     /gene="argR"
                     /locus_tag="OO161_003637"
     CDS             complement(4307..4777)
                     /gene="argR"
                     /locus_tag="OO161_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5464548.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            5212..6150
                     /gene="mdh"
                     /locus_tag="OO161_003638"
     CDS             5212..6150
                     /gene="mdh"
                     /locus_tag="OO161_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5464549.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         1512 a         1442 c         1597 g         1635 t
ORIGIN      
        1 agataacagc ataacagtta cgctaatata ttaaatatca atctacagca atgttgctct
       61 cgcccggctt tccatgccct tctctgcggc gacagatgct gaaaataata acgcctgctc
      121 tctctataca accaaggtca acatgagtct gtttcccgtt atcgtggtgt ttgggctgtc
      181 cttcccaccg atattttttg aattgctttt atcactggcg attttctggc tggtgcgccg
      241 ggtacttgtg ccaacaggta tctacgactt tgtctggcat ccggcgttgt tcaacaccgc
      301 gctctattgc tgcttgtttt atttgatatc gcgactgttc gtttgaggtt gaagtgaaaa
      361 cactaataag aaaattctcc cgtacggcca tcacggtcgt attagtcatt ctggccttca
      421 tcgcaatttt taatgcctag gtctattaca ccgaatcccc ctggacgcgt gacgcgcgct
      481 ttagcgctga cgtcattgcg atcgcgccgg acgtttctgg actcattacc taggtgaatg
      541 ttcatgataa ccagctggtg aaaaaaggac agatactgtt caccatcgac cagccgcgct
      601 atcaaaaggc gcttgaggaa gcgcaagccg atgttgctta ttatcaggta ctggcacagg
      661 agaaacgcca ggaggccgga cgtcgtaacc gtctcggtgt gcaggcgatg tctcgcgaag
      721 agatcgacca ggctaacaac gtactacaaa cggttctgca tcagttagcg aaagcgcagg
      781 cgacccgcga tctggcaaaa ctggatcttg aacgcacggt gatccgcgcg ccagcagatg
      841 gctgcgtgac caacctcaac gtctataccg gtgagtttat tactcgagga tcaacggcgg
      901 ttgcgctggt gaaacagaac tccttctatg tactggccta tatggaagaa actaagctgg
      961 aaggggtgcg tccggggtat cgtgcagaga tcacgccgct tggcagtaac aaagtgctga
     1021 aagggactgt tgatagtgtt gccgcagggg tcaccaacgc cagcagcacg cgtgacgaca
     1081 aagggatggc gactatagac tctaaccttg aatgggtgcg tcttgcgcaa cgtgttccgg
     1141 ttcgtattcg tctcgacaac cagcaagaga acatctggcc tgcgggcacc actgctacag
     1201 tggtggtcac tggcaaacaa gatcgcgacg aaagccagga ttcgttcttc cgtaaaatgg
     1261 cccatcgcct gcgtgagttt ggttaatcac gatgggtatt ttctccattg ctaaccaaca
     1321 tattcgcttt gcggtaaaac tggcgaccgc cattgtactg gcgctgtttg ttggctttca
     1381 cttccagctg gaaacgccac gctgggcggt actgacagcg gcgattgttg ccgccggtcc
     1441 ggcctttgct gcgggaggtg aaccgtattc tggcgctatt cgttatcgtg gctttttgcg
     1501 catcatcggc acatttattg gctgtattgc cggactggtg atcatcattg cgatgatccg
     1561 cgcaccatta ttgatgattc tggtgtgctg tatctgggcc ggtttttgta cctggatatc
     1621 ctcgctggta cgaatagaaa actcgtacgc gtgggggctg gccggttata ccgcgctgat
     1681 cattgtgatc accattcagc cggaaccatt gcttacgccg cagtttgccg tcgaacgttg
     1741 tagcgagatc gttatcggta ttgtgtgtgc gattatggcg gatttgctct tttctccgcg
     1801 atcgatcaaa caagaagtgg atcgagagct ggaaagtttg ctggtcgcgc aatatcaatt
     1861 aatgcaactc tgtatcaagc atggcgatgg tgaagttgtc gataaagcct ggggcgacct
     1921 agtgcgacgc accacggcgc tacaaggcat gcgcagcaac ctgaatatgg aatcttcccg
     1981 ctgggcgcgg gccaatcgac gtttaaaagc gatcaatacg ctatcgctga cgctgattac
     2041 ccaatcctgc gaaacttatc ttattcagaa tacgcgcccg gaattgatca ctgatacttt
     2101 ccgcgaattt tttgacacgc cggtagaaac cgcgcaggac gtccacaagc agctcaaacg
     2161 cctgcggaga gttatcgcct ggaccgggga acgggaaacg cctgtcacca tttatagctg
     2221 ggtcgcggcg gcaacgcgtt atcagcttct caagcgcggc gttatcagta acacaaaaat
     2281 caacgccacc gaagaagaga tcctgcaagg cgaaccggaa gtcaaagtag agtcagccga
     2341 acgtcatcat gcgatggtta acttctggcg aaccacactt tcctgcattc tgggcacgct
     2401 tttctggctg tggacgggct ggacttccgg cagtggtgca atggtgatga ttgcggtagt
     2461 gacgtcactg gcaatgcgtt tgccgaatcc acgcatggtg gcgatcgact ttatctacgg
     2521 gacgctggcc gcgctgccgt tagggctgct ctactttttg gtgattatcc ctaataccca
     2581 acagagcatg ttgctgctgt gtattagcct ggcagtgctg ggatttttcc tcggtataga
     2641 agtacagcaa cggcgactgg gctcgatggg ggcgctggcc agcaccataa atattatcgt
     2701 gctggataac ccgatgactt tccatttcag tcagtttctc gacagcgcat tagggcaaat
     2761 cgtcggctgt gtgctcgcgt tcaccgttat tttgcttgtg cgggataaat cgcgcgacag
     2821 gaccagacgt gtactgctta atcagtttgt ttctgccgct gtttccgcga tgactaccaa
     2881 tgtggcacgt cgtaaagaga accacctccc ggcactttat cagcagctgt ttttgctgat
     2941 gaataagttc ccaggggatt tgccgaaatt tcgcctggcg ctgacgatga ttatcgcgca
     3001 ccagcgcctg cgtgatgcgc cgatcccggt taacgaggat ttatcggcgt ttcaccgaca
     3061 aatgcgccgc acagcagacc atgtgatatc tgcccgtagc gatgataaac gtcgtcggta
     3121 ctttggccag ttgctggaag aacttgaaat ctaccaggaa aagctacgca tctggcaagc
     3181 gccaccgcag gtgacggaac cggttcatcg gctggcgggt atgctccata agtatcaaca
     3241 tgcgttgacc gatagttagg tcaaaaccga cgccaaaagt gtcggttttt tcatggctat
     3301 acttagcgat gcacggcaga actcgccgcg aaacgtgacg gtggcaatag atgaatattt
     3361 atacctttga ttttgatgag attgatagtc aggaggattt ttatcgtgac tttagccaaa
     3421 cctttggtct ggcgaaagat aaggtacgcg atctcgactc actatgggat gtgttaatga
     3481 acgatgtcct gccgctacca cttgagattg aatttgttca tctgggagag aaaacgcgtc
     3541 gccgttttgg cgcgttaata ttgctgtttg atgaggcaga ggaagagctg gaagggcatt
     3601 tgcgttttaa tgttcgtcat tagcgcaaaa aaaagccccc gaaccggggg caatatcgtc
     3661 ggacaagacg atgagggttt atttgtacag ttcagccgta gcgtgccagg tgtcaccgct
     3721 acgagcttca gtaatctggt aggccgttgc gcctttctct tccgcttttt tgttcagcat
     3781 ttcacgcata tccattggcg aagacgccac accacttacg gatacggtcc cgattgcttc
     3841 acgattttgt gcttgtgcag catcaatgga gtcggcagcg aatgcaccga aagagagaac
     3901 agaaagtacg cttaatgcag ctacagtggt tttgattttc atgattttta cctcgacata
     3961 atcttttagc tgggcctttg tttcgtgacc catatcacaa aatcaagcat acactaatta
     4021 ttgaaccaat taatatcacg ctaactgttt cagttgatac taagtgttaa aaatgtgaat
     4081 tttaataaca aaaaagaatt aaaaacacgg tcatttattt ttaattctag tattttcaat
     4141 tagataatga attttgcata atgtgctttt tttaccgtat gtattcatta tgtgaatgac
     4201 atgtcgcagt aaaacgcact attcgttaaa ttaatcgcag gggaaagcag ggggttgaga
     4261 gggataagca acattttccc cgccgccaga agcgacgggg cagagattaa agctcctggt
     4321 cgaacagctc taaaatcgct tcgtacaggt ctttgacggt gaaaccgtta gcaggggtgg
     4381 taaagatggt gtcatcgcca gcgatggtgc ccagaatacc ttctgctttg cccagtgagt
     4441 ccagcaggcg agcaattaac tgcgccgcgc cagggctggt atgaatcacg acaactgcat
     4501 cgttgtagtc gatatccagc accagattct tcaatggact ggaggtggtt ggtacaccca
     4561 gttcagctgg caggcagtaa accatttcca ttttggcatt gcgtgtacgt acagcaccaa
     4621 acttggtcaa catccgcgag actttagact gattaatatt gtcaaagcct tgctcctgca
     4681 acgcggcgac gatttcgccc tgggagctaa atttctcttc tttaagtaat gctttaaatg
     4741 ctttaactag ttcttcttgc ttagccgagc ttcgcataag tcacccgata tggtggttga
     4801 tacaacatta ttgtgcatac agatgaattt ttatgcaaac agtcagccct gaagaaggct
     4861 gaaataatgt tatgaaagag cgggatttta tcaaatttcg ttattgagaa acatgcctgc
     4921 gtcacggcat gcaaattctg cttaaaagta aattaattgt tatcaaattg atgttgtttt
     4981 ggctgaacgg tagggtatat tgtcaccacc tgttggaatg ttgcgctaat gcataagcga
     5041 ctgttaatta cgtaagttag gttcctgatt acggcaatta aatgcataaa cgctaaactt
     5101 gcgtgactac acattcttga tatgtggtca ttgtaaacgg caattttgtg gattaaggtc
     5161 gcggcagcgg agcaacatat cttagtttat caatataata aggagtttag gatgaaagtc
     5221 gcagtcctcg gcgctgctgg cggtattggc caggcgcttg cactactgtt aaaaacccaa
     5281 ctgccttcag gttcagaact ctctctgtat gatatcgctc cagtgactcc cggtgtggct
     5341 gtcgatctga gccatatccc tactgctgtg aaaatcaaag gtttttctgg tgaagatgcg
     5401 actccggcgc tggaaggcgc agatgtcgtt cttatctctg caggtgtagc gcgtaaaccg
     5461 ggtatggatc gttccgacct gtttaacgtt aacgccggca tcgtgaaaaa cctggtacag
     5521 caagttgcga aaacctgccc gaaagcgtgc attggtatta tcactaaccc ggttaacacc
     5581 acagttgcaa ttgctgctga agtgctgaaa aaagccggtg tttatgacaa aaacaaactg
     5641 ttcggcgtta ccacgctgga tatcattcgt tccaacacct ttgttgcgga actgaaaggc
     5701 aaacagccag gcgaagttga agtgccggtt attggcggtc actctggtgt taccattctg
     5761 ccgctgctgt cacaggttcc tggcgttagt tttaccgagc aggaagtggc tgatctgacc
     5821 aaacgtatcc agaacgcggg tactgaggtg gttgaagcga aagccggtgg cgggtctgca
     5881 accctgtcta tgggccaggc agctgcacgt tttggtctgt ctctggttcg tgcactgcag
     5941 ggcgaacaag gcgttgtcga atgtgcctac gttgaaggcg acggtcagta cgcccgtttc
     6001 ttctctcaac cgctgctgct gggtaaaaac ggcgtggaag agcgtaaatc tatcggtacc
     6061 ctgagcgcat ttgaacagaa cgcgctggaa ggtatgctgg atacgctcaa gaaagatatc
     6121 gccctgggcg aagagttcgt taataagtaa ttgattagcg aataataaaa aaccggagca
     6181 cagact
//
LOCUS       DAJTNA010000211         6051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTNA010000211 DAJTNA010000000
VERSION     DAJTNA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.214"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            335..697
                     /gene="queD"
                     /locus_tag="OO161_003639"
     CDS             335..697
                     /gene="queD"
                     /locus_tag="OO161_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5464550.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            775..2043
                     /locus_tag="OO161_003640"
     CDS             775..2043
                     /locus_tag="OO161_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5464551.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSHVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMDMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            2034..2294
                     /locus_tag="OO161_003641"
     CDS             2034..2294
                     /locus_tag="OO161_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5464552.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            2311..2886
                     /locus_tag="OO161_003642"
     CDS             2311..2886
                     /locus_tag="OO161_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR5464553.1"
                     /translation="MPLLHLLRQNPVIAAVKDNANLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLITGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(3034..3894)
                     /locus_tag="OO161_003643"
     CDS             complement(3034..3894)
                     /locus_tag="OO161_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5464554.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGIQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAKVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(3891..4670)
                     /locus_tag="OO161_003644"
     CDS             complement(3891..4670)
                     /locus_tag="OO161_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5464555.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWTCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(4648..>6051)
                     /locus_tag="OO161_003645"
     CDS             complement(4648..>6051)
                     /locus_tag="OO161_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5464556.1"
                     /translation="GRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRIAA
                     LTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWISDH
                     IGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFSPR
                     RHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWGTP
                     ESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFNSV
                     FFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHRKF
                     LLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIRSL
                     GVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKALP
                     LVAAGNVGGANEHSVSV"
BASE COUNT         1479 a         1636 c         1537 g         1399 t
ORIGIN      
        1 tttcattgga gcacctgtgt tgttctgagg tgagcatatc acctctgttc aggtggccaa
       61 attcagtgtg ccacttcaaa gaagataagg ttaacggggt gaacggtgtg gattaaagac
      121 gggatagcga taactaataa ccaaatcgac taacctgttt tagcaatagt ctttaacaac
      181 aaaatagatt aaccaaccca atgaaaaaca aatgaattta gccaatcatt aagataaatc
      241 agcgattttg cgcaacaaag tcgttttaga taatgcgaaa aaacagcctt tccggtactc
      301 tacggcggtt ttatcgtcct gtagagaaat tatgatgtcc accacgttat ttaaagattt
      361 caccttcgaa gccgctcacc gcttaccaca cgtcccggaa gggcataaat gtggtcgcct
      421 gcacgggcat tcctttatgg tgcgactgga aattaccggg gaagtcgatc cgcatacggg
      481 ctggattatc gatttcgctg aactaaaagc ggcgtttaag ccaacttatg agcgcctcga
      541 tcaccattat ctcaatgata ttccaggtct ggaaaaccca actagcgaag ttttagcaaa
      601 atggatttgg gatcaggtta aacccgttgt gccgctgtta agtgcggtga tggtaaaaga
      661 aacctgcacc gctggttgta tctatcgcgg cgaatgataa gagtgtgtcg gcggtcaatt
      721 tcccttaagt aacgctatgt tagggtattg tgttcaggat atctggggca tgacatggaa
      781 gacgactgcg acattattat tattggtgcc ggtattgcag gcaccgcttg cgcgttacgc
      841 tgcgcgcgag cgggtttatc cgttttgtta ctggaacgcg ctgaaatccc cggcagcaaa
      901 aatctttccg gcggacggtt atatacccat gcactcgcgg aactcctccc tcagtttcat
      961 ctgaccgcgc ctcttgaacg acgcatcact cacgaaagcc tttccctgtt aacgcccgat
     1021 ggcgtaacga cgttttccag cttacagccc ggcggtgaat cctggagtgt attacgtgca
     1081 cggttcgatc cgtggctggt tgccgaagcc gaaaaagaag gtgtcgaatg tatccccgga
     1141 gcgacggtgg atgcactgta tgaagaaaac ggcagagtct gtggcgttat ttgtggtgac
     1201 gatattctcc gcgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt cctggctgaa
     1261 cgtcacgggt tagtgactcg tcctgctggc gaagcgatgg cgttggggat caaagaagtg
     1321 ctgtcgctgg aaacatccgc tattgaagaa cgttttcatc tggagaataa cgaaggcgca
     1381 gcgttgctgt tcagcggcgg aatctgtgat gacttacccg gcggcgcatt tctttatact
     1441 aatcaacaaa cgctctcgtt agggattgtt tgcccgctct cttcccttac gcaaagtcat
     1501 gttccggcaa gcgagctgct gactcgcttt aaagcgcatc cggcagtgcg cccgcttatc
     1561 aaaaacacgg aatcactgga gtatggtgcg catctggtgc cagaaggtgg cttgcacagt
     1621 atgccggtgc aatacgccgg taacggctgg cagctggtgg gcgatgcgtt gcgcagttgc
     1681 gtcaataccg gaatttccgt gcgcggcatg gatatggcgc tgactggcgc gcaggcggca
     1741 caaacgctga taagcgcctg ccagcaccgc gagccgcaaa atctgtttgc gctttatcat
     1801 cacaacgtag agcgcagcct gctatgggat gttctacaac gttatcagca tgttccggcg
     1861 cttttgcaac gccctggctg gtatcgggcg tggcctgcgt taatgcagga tatttcccgc
     1921 gatttatggg atcagggtga taaacctgtt ccaccgctgc gccagttatt ctggcgtcat
     1981 ttacgtcgtc atggcctgtg gcatctggcg ggcgatgtta tcaggagtct gcgatgtctg
     2041 tagcccataa tctctggcgc gttgctgatg cgccgcacat tgttccggct gactccgttg
     2101 agcgtcagat ggtacaatgg ttgattaacg cctgtccggc aggtcttttt tcgctcacac
     2161 cggaaggtga cttacgcgtt gattatcacg gctgcctgga gtgtggcacc tgccgtttgc
     2221 tgtgcgacga atccacacta caacagtggc gctatccgcc ttccggattc ggcatcacct
     2281 accgctttgg ataaataata aagataattt atgcccctct tacacctgct ccgccagaat
     2341 ccggtgattg ctgccgttaa agataatgcc aacctgcaac tggcaatcga ctctgaatgc
     2401 cagtttattt ccgtgttgta cggcaatatc tgcaccatca gtaatatcgt taaaaagatt
     2461 aagaacgccg ggaaatatgc ttttattcat gttgatttgc tggaaggcgc gtcaaataaa
     2521 gaagtcgtga ttcagttttt gaaactggtg accgaggcgg acggcattat cagtaccaaa
     2581 gcctcaatgt tgaaagcggc aagagcggaa ggttttttct gtattcatcg cctgtttatt
     2641 gttgattcaa tttcgtttca caacattgat aagcaagttg cgcaatcgaa tccggattgt
     2701 attgagatcc tgccaggctg tatgcccaaa gtgctgggct gggtgacaga gaaaatccgc
     2761 caaccgctga ttaccggtgg gctggtgtgc gatgaagaag atgcgtgtaa tgcgattaac
     2821 gcgggtgtcg tggcgctttc caccacgaat accggggtct ggacgttagc gaaaaaatta
     2881 ctttgacggg ataatcgtaa ccaattgaat ttggtttgat ttgtaggccg cacgccacat
     2941 ccgacattca gcgcctgatg cgacgcttga cgcgtcttat caggcctaca agtccccagg
     3001 ccagaaccgt aggcaggatc atccggcaac acattattgg caatctgcgt gaatgtttgt
     3061 caccagtgcc tccagcacca ctttccagtc atcaaccacg ccaacatctg cctgtgaaaa
     3121 taccgcagcg ctggcgtcat gattaatcgc caccacaaac ttgctattgc gtacgcccgc
     3181 cattaacgct gctgcgccgg aagcgccaac aacaatacac acctcaggtg ccagcaaatg
     3241 tccggaaata ccaatcactt tttcagcatc aacgccaccg ttcatcactc gcgcccgact
     3301 gtagcccact ttggctccca gcttttctgc cagcatagcg atttcctggt tatctgcctc
     3361 tccgccctgc cccaccacca gcacccgtct ggcctcagct aatgggtcgc gagtaacatt
     3421 tttcagatcc tctgtgctaa ccagccagtc gggaagcgca cccgggacaa tgttcagttg
     3481 ttgtattcca gagggcaacg tggcgttttt cgctgctccc gcctgacgcg ccagggaaag
     3541 gcatagcggg cgtttttctg tttgcagcgt cgccgttagc gcatttcccc agtgggattt
     3601 gcgcacgctt acggttggaa tatctaacga agtcacctgg cagatgcttg caccatgtaa
     3661 acgccaggcc agccgggtcg acagttcatc gccaaacgtt cccggcggaa agagaacaac
     3721 gtccgcaggc gttcgctgcc actgttccac caacgcatcc agcacctgtt ccgccactac
     3781 aggctgaggt tcaatctgcc agtgcgccag ggtacaacca ctgaaatcct gcgccgccag
     3841 ccagcttgct attgcggcat tctcctgatt tattgtcaca attgcgatat tcatggctgc
     3901 atcctttggc gtaaatagtc ctgccatagc ttttgagctt tttctgctac cgtttgccca
     3961 tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca gttgcagaca ctgcatcgct
     4021 ggcatttcgg cagcaacagt tttacggata atttctgctt tccccgcagc cattcgctgg
     4081 cgcattcccg gtactggcaa ggccacttca ccgcactgac gcacggcaat caccgcaggc
     4141 aagcgaaccc ggcaacaacg caacccatgt tcagtgcgct gttcgagggt gataaacagc
     4201 gcgtcgagag tgaaacgctc cacctgggtg aagcaggtcc agcccagcat ttccgccagc
     4261 aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc agccggtaat tatcagatcc
     4321 agtggattct gatgctgcca ttcggcaata tggcgagcga caaattccgg cgcaaagcgc
     4381 agatctgccg ccgtctccag caatacagct tcctcaaacc caagagccat gagatagcgc
     4441 agccagtgca atgcccgttc atcccccatg ctcaacgcgg ttaaagacat cggcgtaccg
     4501 ttttttctct gcgccaacag cagcgcggca gcggcctgtt catcagcacc gagtaaactt
     4561 cgcagtagcg aaatatccgg tccgctttta ccctgagccg ccgcctgcca ctctttttcc
     4621 gccagcattc cggcatccgg ttcggcttta aacgctaaca gaatgttcat tcgcacctcc
     4681 tacatttccc gccgccacca gcgggagggc tttagtttct ggtgcccata gccaggtcac
     4741 aacaaaacca accaacaaca ctgtcgccag gagcaataag gtgacttgca ttccccactg
     4801 cgccagcacc cacggcagca ggccagtact taccgccgcg ccaagtcgac tcatggcggt
     4861 ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa ctttccgcag gcaaaatgcc
     4921 caccagatta ctgactgccg aaatggtggt gctgaagaga acaaaaagca gcagcgttaa
     4981 tgaactgccg gaaggcaaac aggccattac taccagcgtt gccgccagca gcaaaaaact
     5041 tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga actaatccca gcagcgcgcc
     5101 cacaattaac aacgcattaa gcatcaggct ggcagtcagc gcatcttcca gaccaatagt
     5161 ctgggcgata gttggcagcc aggtataaat cacaaaccat gggattacga gacagacaaa
     5221 gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa gagaacaagg ttttgatgtg
     5281 tttatgggtc gccgttacca cttcatcgcc cagtaaaaca tggggaccaa aatagcgatg
     5341 cacgatagcg tgagcttctg caaaacgccc ctggcgcagt agccagcgtg gtgattctgg
     5401 cgttccccag cgtaataacg taatcaacaa cgcgggcaga gctgccgatg ccagtagcca
     5461 gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg gcaatacttg ccagcacata
     5521 gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata ccgcgatggc ggcgcgggga
     5581 aaattcagcc agcaaggtgt gacctactga ataatcgcct cccagaccaa tgccaatcaa
     5641 aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa aattgtaaaa acgaagcaag
     5701 cgtaatcagc aaaaagctga aggtgaagat tttttgccga ccaatatggt cggagatcca
     5761 cccaagaacc aggctgccaa ggaacaaacc aaggagcgcc gagccgccga tcattcccgc
     5821 cataaacggc gtcagttgca tggcgggcgt aagctgaata atggcgtaac caatgacgcc
     5881 gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc gcagcaatgc ggcagtgaaa
     5941 acggttaagc ggtaaatcat ccattcgcac cggtgaagtg ttcatcttta ccccttcatt
     6001 gtccggtaat aagaacggct tctcgcctga gaagccgaaa aggcaacgcc c
//
LOCUS       DAJTNA010000212         6040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTNA010000212 DAJTNA010000000
VERSION     DAJTNA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.215"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(86..1072)
                     /gene="araH"
                     /locus_tag="OO161_003646"
     CDS             complement(86..1072)
                     /gene="araH"
                     /locus_tag="OO161_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5464557.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVICGLILLAAVIFDRYKQKAKRTV"
     gene            complement(1087..2602)
                     /gene="araG"
                     /locus_tag="OO161_003647"
                     /pseudo
     CDS             complement(1087..2602)
                     /gene="araG"
                     /locus_tag="OO161_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
     gene            complement(2672..3661)
                     /gene="araF"
                     /locus_tag="OO161_003648"
     CDS             complement(2672..3661)
                     /gene="araF"
                     /locus_tag="OO161_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR5464558.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MVITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKVQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            4375..4878
                     /locus_tag="OO161_003649"
     CDS             4375..4878
                     /locus_tag="OO161_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5464559.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4957..5208)
                     /locus_tag="OO161_003650"
     CDS             complement(4957..5208)
                     /locus_tag="OO161_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5464560.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(5323..5409)
                     /gene="azuC"
                     /locus_tag="OO161_003651"
     CDS             complement(5323..5409)
                     /gene="azuC"
                     /locus_tag="OO161_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5464561.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5672..5995
                     /locus_tag="OO161_003652"
     CDS             5672..5995
                     /locus_tag="OO161_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5464562.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         1582 a         1547 c         1366 g         1545 t
ORIGIN      
        1 gtcgccattt gcaacgaaga acagccaacc gttatggcgg ttggctgtgg tgggaaaaaa
       61 cgctaaattg ttgcagaaaa aagcatcaga cagtgcgttt cgctttttgc ttgtaacggt
      121 cgaagatcac cgctgccagc aggattaagc cgcaaatcac gtactgcgcg aaaggagaaa
      181 tattaagcag gttcatggcg ttttccacgg tgcctaaaat taagataccc gccaccacat
      241 atgagatttt tccgatgcct cctttcagag aaacgccacc taaaacgcag gcggagataa
      301 caatcagctc gtaaccaatc gacgtcattg gctgcccact ggtcatacgt gaagccagaa
      361 taattccggc tatcgcagat accaggccag agagaacaaa gataataatt ttggtgcgaa
      421 caaccggtac acccgccaga cgcgcagcct cttcgttccc gccaatcgcc agggtgttac
      481 gaccaaaggt ggttttattc agcaacaaac caaagataat cagacacgcg acggtgagcc
      541 agattggcgc aggcagaccg aaccagttag cgtaaccaag ggcaaagaag ctttcatctt
      601 cgataccgac cgctttaccg tctgaaatga tgtatgccag acctcgaaca atctgcatcg
      661 ttgccagtgt tgtgatcaga gcatttattt tcagtttggc gataacaaag ccattgacca
      721 ggccacagag aatgccaagc agcaaccccg ctgccacgcc aatccacagg ctttcagtca
      781 ggttgataac caccgccgtg gtgacacccg cacaggcaat tacggaggcg acagaaaggt
      841 caaagtcacc ggaagcaagg cagaacaaca tgccacacgc caccatcccc gacatggaaa
      901 ttgccaggcc caaccctttc atattaatga aggtggcaaa atttgggaca aaaatggcac
      961 aggcgataaa gagcaccgca aacaccacca gcatgccgta ctgatcccag atacgcccga
     1021 agctgaatga cgacttaggt gcgccagaac ccgatgtaga aacagaagac atcatactct
     1081 ccttactcag gcaactgcct ggctgacttt aggcatcgca aggctcagtg cctgacgctc
     1141 atctgcctgc tcgtgtaaca attcaccggc gatttcacct tcccgcatca ccacaatccg
     1201 gtcggcaacg ccgaggactt caggtaagtc gctggaggca aacagcaccg ccacaccctg
     1261 cgccgccagc gcataaatta cgttatatat ttcgtgctta gcaccaacat caatgccgcg
     1321 cgtaggttca tccagcaaaa tgaccttcat ctcttccgat aaccagcggc ccagaatggc
     1381 tttttgctga tttccgcctg agagattcat gatcagttgc tcagcgcccg gcgttttgat
     1441 gttgagcgaa cgaatgtgct gatcggcatt gttttcttcc caaccgttgt tgattacaca
     1501 accgccgagc acatgtttgc gtctggcact gatgttgata ttgtcgcgaa cggagtgcac
     1561 gggaataatg ccttccgctt tgcgatcttc cgggcagagc atcatgcctg cggcaatggc
     1621 gtggctcggt ttacgaatat cgatcggctg ttggtcgata taaacctgac cggcggtgat
     1681 ttgcgtcccg ccaaacaagc ctttcattaa ttcgctacgc cccgccccta ccagaccaaa
     1741 cagcccaaca atttcaccac tgcgaaccgc cagacttatt ggcgtacgca cgcctggtgc
     1801 tttcacagca tcaagacgca ggcgctcctc gccataacta cgaggttgcc agccgtagat
     1861 atcgccaatg tcgcgcccga ccatcgcctg caccagcgcg tcgtggtcaa cctgctgcat
     1921 atcggtaaag gttttgacat aacgtccatc tttaaagaca gtgatggcat cgctgagggc
     1981 aaatatttct tccatacggt gagaaacgta taagatcacc cgcccctctt ttcgcagttc
     2041 acgaataacg cgggaaagat tgtcgatttc gcgggcagaa agggagctgg ttggctcatc
     2101 aaaggcgata attttagcgt tacgcgccag cgccttggcg atttcaacca tctgccactg
     2161 accaatggag agatatttca gcggcgtgtc cgggtcaata tccataccaa gatgtttaag
     2221 ttgtaaaccc gcctcataat tcagcaatga gcgattcaca atgccgcctt tatgcggcag
     2281 ctggccgaga tagatgtttt ccgcgacggt catttccggc acgagatgca gttcctggta
     2341 aataatcgcc acacccgcgt taagtgctgc ggtcgtgtcg gaaaaggaca tttcctgccc
     2401 attaatcact acagaacccg tggttggcgc atagttgccg ctgaggattt tttaagagag
     2461 ttgattttcc tgcgccattt tcacccatca acgcatgaac ctgaccggca tagcagtcaa
     2521 aactaatatc cgtcagcgcc ttaacgccgg gaaacgtttt accgatgccg cgaaatgaga
     2581 gatacggggt agactgttgc ataacgtctc cgtgaatcac tggtcgttca caccccctct
     2641 gcatcatgca gaggggaatt tttccggcaa attacttacc gcctaaacct tttttctcca
     2701 gttcttcttt aaagttgtca cgcgtgatca gcaccacgtc ggtaacttcg gtaaattttg
     2761 gcggttcaac gtcttttgct acccagttgt aaagcatttc gctggattta tagccatgta
     2821 cgtccgggct tggcagcagg gaaccgtaga agccggttgc ctgtacttta gacagttcgc
     2881 tcaccgcatc cacaccgtta atgccaatgc cgataatatc ggcagcttta aagccctgac
     2941 cttccgtcgc gcgtacgccg cccagcacgg tgctgtcgtt cataccgacg atcagccaat
     3001 gtttaacttc aggatgttga accagcattg agttggcagc gtcaaatgcc cccgggatgt
     3061 cgttagattt ggtaggtacc tgataaattt gtttttccgg gaatccggcc gctttcagcg
     3121 catccataga tcccgtagta cgccggcggg cggtatccag ttcgttggcg gtaatcacca
     3181 tcaccgcgct ttctttgaca tcccagccac gtttctgcat ctctttatac agttcctgac
     3241 cctgacgttc gccaatttta gtcgccgcca tcatcaccag cggaacggta tccattggct
     3301 tacctttggc gttaacaaac tgatcatcca cggcaatgac tttcatatcg tagccacgcg
     3361 ctttcgcgac gatggcagag ccgagtttgg ggtccggagt acaaataacg aaaccttttg
     3421 cgccactggc agccaggctg tcgatcgcgt tcaatgtttt ttcgccatcc ggcacggcaa
     3481 tcttaataac ctcaaaccct aaatccttcc cggctttatc ggcaaacttc cattcggtct
     3541 ggaaccacgg ctcttccggt tgcttcacca gaaaaccgag cttcaggttc tccgccatag
     3601 cggattgtga cataacggct gccagaccaa tggctgccag ggctttagta aatttgtgca
     3661 tggttctctc cagctttagt gtcgttttgt gtagggcaaa atcgaatgac attcgttaaa
     3721 ttaatcggaa aacaaagcat taccttttaa ctaaaagata agtgaccgtg ttgacatagt
     3781 tttagcgaga aattaattct ccataggaga gcaatatcac atcgcagaat tacagtgaga
     3841 acgtgcataa atttagcggg aaaagacata agggaaagcc aatttgtcag acaaattgtc
     3901 gaatgcacag cagattaatc cataagatta gcttggaaat ccttgttgtc tttggtaccc
     3961 atgcgggatg tcttcttttt aaccagtcaa taggccgcat tacctggcgt tgagtttttg
     4021 aaatggtgta ataaccgcaa ctcaaagatg tggaaaatgc acgtcattca tttcgccatt
     4081 aattatcact gtgctcatta attaacagaa cacgtataat gagagccatc tcgcaaaaat
     4141 gaaaaaacgt tttataaaat catcatttca tcaatgaaat gtaaaaatat ataaacttga
     4201 tgatttaagc attttcttat acccgttcag acgttattct tatttcagat catcgtcaga
     4261 attgactcca cgatcacatt tcggaccggc agaaaggaat tattctgcaa acagtaatta
     4321 tggtgttttg atttatcttg cacctctcca cttctggata taaggatatt aggtatggca
     4381 accgctggaa tgcttctcaa actcaactct caaatgaacc gcgagtttta cgcatccaat
     4441 ctctaccttc acctgagtaa ctggtgttct gaacagagtc tgaacggcac cgccactttc
     4501 cttcgcgccc aggcacagag taatgtgacc caaatgatgc gcatgtttaa ctttatgaag
     4561 agtgtcggcg ctacccccat cgttaaagcc attgatgttc ccggtgaaaa actgaactct
     4621 ctggaagaac tgttccaaaa aacgatggaa gaatacgagc aacgttctag tacgttggca
     4681 cagttagccg atgaagcgaa agaactgaat gatgattcaa ccgtcaattt cctgcgcgat
     4741 ctggaaaaag aacagcagca tgatggtctg ttgctgcaaa ccattcttga tgaagtgcgc
     4801 agtgcgaaac ttgctggtat gtgcccagtg cagaccgacc aacatgttct gaatgtcgtg
     4861 tcgcatcagc tgcattgatc atacatcggc gctaatgcat tgcgccgatg aagtttttga
     4921 gaaaccgctg cctcatcttt ttgaagcagc ggttttttaa tgggattcgc cttgtggggt
     4981 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     5041 ggcggcggga tggttactaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     5101 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     5161 aaccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     5221 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     5281 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     5341 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggatcttgcg
     5401 cagtttcatc gcggcactcc gaccatttgt tatacatgtg ttattttctt tgcgcttata
     5461 atatgacaac catcacaaaa atcaatcttt atgtgatgaa aatcacacaa ataccccctt
     5521 aatgttataa aaatgataat caaaaaacag cccccccatt tctgacacct acagatggca
     5581 agaaatagcg cctgccaggc gtcttttccg gtcattgtcg cagcgctgta acgcgtaaaa
     5641 tagtgctttc ttttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     5701 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     5761 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     5821 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     5881 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     5941 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     6001 ttaatcagcg aggggatctt cgctgattaa agaaatagcg
//
LOCUS       DAJTNA010000213         5996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTNA010000213 DAJTNA010000000
VERSION     DAJTNA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.216"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            191..865
                     /locus_tag="OO161_003653"
     CDS             191..865
                     /locus_tag="OO161_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5464563.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSQGRLINFPEQPEFSEEERKRYNGMAMDYVLVSKKNNKIVCVI
                     ELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPLVAAT
                     KSESVH"
     gene            complement(956..1092)
                     /locus_tag="OO161_003654"
     ncRNA           complement(956..1092)
                     /ncRNA_class="other"
                     /locus_tag="OO161_003654"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1137..1224)
                     /locus_tag="OO161_003655"
     tRNA            complement(1137..1224)
                     /locus_tag="OO161_003655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1188..1190),aa:Ser,seq:gga)
     gene            1458..2396
                     /gene="ghrA"
                     /locus_tag="OO161_003656"
     CDS             1458..2396
                     /gene="ghrA"
                     /locus_tag="OO161_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR5464564.1"
                     /translation="MDIIFYHSTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPHVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            2451..3188
                     /gene="ycdX"
                     /locus_tag="OO161_003657"
     CDS             2451..3188
                     /gene="ycdX"
                     /locus_tag="OO161_003657"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5464565.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3212..3766
                     /gene="ycdY"
                     /locus_tag="OO161_003658"
     CDS             3212..3766
                     /gene="ycdY"
                     /locus_tag="OO161_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5464566.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3868..4359
                     /locus_tag="OO161_003659"
     CDS             3868..4359
                     /locus_tag="OO161_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5464567.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4423..5256)
                     /gene="csgG"
                     /locus_tag="OO161_003660"
                     /pseudo
     CDS             complement(4423..5256)
                     /gene="csgG"
                     /locus_tag="OO161_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(5283..5699)
                     /gene="csgF"
                     /locus_tag="OO161_003661"
     CDS             complement(5283..5699)
                     /gene="csgF"
                     /locus_tag="OO161_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5464568.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNNYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5724..5996)
                     /gene="csgE"
                     /locus_tag="OO161_003662"
                     /pseudo
     CDS             complement(5724..5996)
                     /gene="csgE"
                     /locus_tag="OO161_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
BASE COUNT         1493 a         1369 c         1506 g         1628 t
ORIGIN      
        1 attctttgct aatattgtgt gttgttattt ttttgtggaa ggcaagccgc aatggctaaa
       61 tagggatggt tgtaataatc aaaacatgat actaactata tattgatcgt tgcattgttg
      121 gcatgtaaca cgctgcagcg agtaacattg gtaatgaaat ataatcaaca tggataggtg
      181 aaaattaatt atgagcttta tggcttttgt aacttgtttg acaattgcat ttatcgccgg
      241 ttttgctgtt gccagaaaat tgacactccg aaaagttgcc tcatcggtaa tcatcgctaa
      301 atcgcaaccc gtcccttcat ctgcaacttc ccctgccgct aaggcgtcga caaggagcta
      361 tataccgcag aaaacctatc gtgactctcg ttggcaagag cgagatgagt tatttcaggg
      421 aatggttgcc gaaggtattt tattggaaag aaaagagtgg ttaatcactc gttgtgaaaa
      481 aatgtatttc gataatctgg aaaaatggtt tggacattat tgtcatatcc attgccaggt
      541 ctctcagggg cgcttgatca actttccaga acaacctgag tttagcgaag aagaaagaaa
      601 gcgttataat ggcatggcaa tggattatgt tctggtttca aagaagaaca ataaaatagt
      661 ctgtgttatt gagctggatg acccgagtca tgaaagggcg gatcgcatta ttcgtgaccg
      721 ttggttggat acaattatga ctcttgcacg ggtgcctttt gtacgagctt ctgtcactaa
      781 attggaggaa attcctccgg tctgggagac tcgtgacaaa attcgcccat tagtcgcggc
      841 gacaaagagt gaatcggtgc attaactaag tatcccggtc ggggcttctc atccccccgg
      901 tgtgtgcaat atacgaaaaa aagcccgtac cttcgtacga gctcttcttt aaagatggcg
      961 gtgagggggg gattgactcg cttcgctcgc cctgcgggca gcccgctcac tgcgttcacg
     1021 gtctgtccaa ctggctgtcg ccagttgtcg aaccccggtc ggggcttctc atccccccgg
     1081 tgtgtgcaat atacgaaaaa aaagcccgta ctttcgtacg agctcttctt taaatatggc
     1141 ggtgaggggg ggattcgaac ccccgatacg ttgccgtata cacactttcc aggcgtgctc
     1201 cttcaaccgc tcggacacct caccaaattg ttgttcctgt cttgctggaa cgggcgctaa
     1261 tttagggaaa tcatgacctg aggtcaacaa actttttgaa aaaatcgcgc gtttattcaa
     1321 acttcaatca atgtgtggtt ttaataagcg aaatctgctt tttttgccac cgaccacgga
     1381 tttgttatgc tggtggcctt tgtagatcat aacgataagt gcgaataaat ttcgcacaac
     1441 gcttttcggg agtcagtatg gatatcatct tttatcactc aacgttcgat acccaatggt
     1501 ggattgaggc actgcgcaaa gctattcctc aggcaagagt cagagcatgg aaaagcggag
     1561 ataatgactc tgctgattat gctttagtct ggcatcctcc tgttgaaatg ctggcagggc
     1621 gcgatcttaa agcggtgttc gcactcgggg ccggtgttga ttctattttg agcaagctac
     1681 aggcacaccc tgaaatgctg aacccttctg ttccactttt tcgcctggaa gatactggta
     1741 tgggcgagca aatgcaggaa tatgctgtca gtcaggtgct gcattggttt cgacgttttg
     1801 acgattatcg catccagcaa aatagttcgc attggcaacc gctgcctgaa tatcattggg
     1861 aagattttac catcggcatt ttgggcgcag gcgtactggg cagtaaagtt gctcagagtc
     1921 tgcaaacctg gcgctttccg ctgcgttgct ggagtcgaac ccgtaaatcg tggcctggcg
     1981 tgcaaagctt tgccggacgg gaagaactgt ctgcatttct gagccaatgt cgggtattga
     2041 ttaatttgtt accgaatacc cctgaaaccg tcggcattat taatcaacaa ttactcgaaa
     2101 aattaccgga tggcgcgtat ctcctcaacc tggcgcgtgg tgttcatgtt gtggaagatg
     2161 acctgctcgc ggcgctggat agcggcaaag ttaaaggcgc aatgttggat gtttttaatc
     2221 gtgaaccctt accgcctgaa agtccgctct ggcaacatcc acacgtgacg ataacaccac
     2281 atgtcgccgc gattacccgt cccgctgaag ctgtggagta catttctcgc accattgccc
     2341 agctcgaaaa aggggagagg gtctgcgggc aagtcgaccg cgcacgcggc tactaataaa
     2401 gcatcaggat tcctgctatc cttggcggga attgaataca ggagagagtt atgtatcccg
     2461 tcgaccttca tatgcatacc gttgccagca cacatgcata tagcacattg agtgattaca
     2521 ttgcccaggc caaacaaaag ggcattaaac tttttgcgat caccgatcat ggcccggata
     2581 tggaagatgc tccgcatcac tggcacttca ttaacatgcg tatctggccg cgagtggttg
     2641 atggggtagg gatcctgcgc ggcatcgaag ctaacattaa aaatgttgat ggtgaaattg
     2701 attgcagcgg taaaatgttt gactcgctgg atctgattat tgccggtttt catgagccgg
     2761 tttttgcacc acatgacaaa gcgaccaata cacaagcgat gatcgccact atcgccagcg
     2821 gcaatgtgca tataataagc catcccggaa atcccaaata tgaaatagat gtgaaagccg
     2881 ttgctgaagc agccgcgaaa catcaggtgg cgctggaaat caataattct tcatttttac
     2941 actcacgtaa gggcagtgaa gacaactgtc gtgcggtagc cgcagcggta cgtgatgctg
     3001 gaggttgggt ggcattaggc tcggattctc acaccgcgtt taccatgggg gaatttgaag
     3061 agtgtcttaa aatcctcgac gcggtagatt ttccgccaga gcgcattttg aatgtttctc
     3121 cgcgccgctt actgaacttc cttgaatctc gcggtatggc accgattgcg gaatttgcag
     3181 acctttaatt actagtaatg gaaatatatt aatgaacgag ttttctatcc tctgtcgtgt
     3241 gctgggttcg ctctattacc gccaaccgca agatccttta ctggtgccgc tgtttaccct
     3301 gattcgtgag gggaaactgg ctgcgaactg gccactggag caggatgagt tactgacgcg
     3361 tttacagaaa agctgtgata tggcgcaagt ctctgccgat tacaatgcgt tgtttatcgg
     3421 cgatgaatgt gccgtgccgc catatcgtag cgcatgggtt gaggacgcaa cggaagcaga
     3481 agtgcgcgct tttctttccg aacgagggat gccactggcg gatacgccag ccgatcacat
     3541 cggcacattg ctgcttgcgg cttcctggct ggaagatcag tcaacggaag atgagagcga
     3601 agcactggaa acactgttca gtgagtatct gttaccctgg tgtggtgcgt tcctcggcaa
     3661 agtggaggcc catgcaacca cgcctttctg gcgcaccatg gcaccgctaa cccgtgatgc
     3721 cattagtgca atgtgggacg agctggaaga agattctgaa gagtaaatgt gatcatcgtc
     3781 actcataact gcaacagata atttgttgtt gcataaaatg tgtgctcgat ctcattcatg
     3841 gccgcgtttt ctgctatcat gcgcggcatg aacatacttc tctctattgc aatcacaaca
     3901 ggtattctct ccggtatctg gggatgggtg gctgtttccc tgggcttact aagctgggcg
     3961 ggcttcctgg gctgtacggc ctactttgcc tgcccgcaag gtgggctgaa agggctggcg
     4021 atctccgctg caaccctgct tagtggcgtg gtgtgggcga tggtcattat ttacggtagt
     4081 gcactggcac cacatctgga aattctcggt tatgtcataa ccggtattgt ggcgtttctg
     4141 atgtgtattc aggccaaaca gctgctgctt tcatttgttc cggggacgtt tataggcgca
     4201 tgcgcgacgt ttgccgggca gggtgactgg aaactggtgt taccttcgct ggcgcttggg
     4261 ctggtatttg gttacgcaat gaaaaacagc ggcctgtggc tggcggcgcg tagtgcaaag
     4321 acggctcacc gtgagcagga aatcaaaaat aaagcgtgag gggcactcac gctttcgctt
     4381 aaacagtaaa atgccggatg ataattccgg cttttttatc tgtcaggatt ccggtggaac
     4441 cgacatatgg cggtatttca ccagaatgtc attctgccgt tctgctttat tttgcaaatc
     4501 ccacagacca cggtcgatac catcattaat caggaaaatg acccctgttt cgatagccga
     4561 catcagacac agcataacgg gttcgtttga ggtgtaaccc acttcccctt ccagcaagcg
     4621 ctggtagtca ataaagcgga aaaccccggc ctgaacttca taggaaagta tcgtcttact
     4681 ggtgttcacc gaggagagga tctcaccggt actcacattg acgacacgca ggttcacggc
     4741 aatctgatcg agctggtatt gcgtgtctgc accgatccca aaatatcttg ccccaacacc
     4801 gcccgattta acgttgcttt cataaccgat aatcgaacct tcaaccataa tatttgccgc
     4861 cgttaaagat tgcagcggga ttcggttatt aatggcaacc gtgccgtttt cttgtgccgc
     4921 acgaataatc ttgcgctcgt taagcaggtt ttgtaagccc tagcgctcca gcggtataaa
     4981 ccagcgagaa tctttcaatg ccgtgaccag cattgccgtg gcgctttgcg gaacagcagt
     5041 ggagaagtta cttgccgggt agggtttaaa ttgcccggtt tcgtcctgaa tgttgtatac
     5101 cgaaacaaag attttacccg tcggcgctgg cagatgggtc aaatctttgt agctctgagc
     5161 acgaggcatt aatgtcggtc tggcggcttc tttaggcggg gcggttaagc atccgctcag
     5221 taacatgacg gcaaccaaaa gaaataagcg ctgcatgatt attttcctta tgaagctggg
     5281 gcttaaaaat cggttgagtt attttgtaaa cccgaaacct ggatggtcga ggtttgtccg
     5341 gttttacgat ctgtcacgtt caactgcaat tgaccatcgc ggttggcaat atcgacaata
     5401 taattgttgg tcaccatgcg gcccggttta ccggtattaa tattcgacag tagcccacct
     5461 aaaatttgtg actggatggc ctgagtaaag ttatctaacg ctgagggtgt ttcaatacca
     5521 aagtcatcgt tatagctcgg atctttataa gagttttggg cctgagcgct atttaataaa
     5581 aaagcgccat tatttgggtt accaccaaag tttggattac ggaactggaa agtcatggtt
     5641 ccagcccaac ttaatggcga aataagcatg agtagaacta ctgcatgttt gacacgcatt
     5701 gcagcctccg aacaattttt tatttagaat tcatcatgcg ccaaatcgcc cgtacttaat
     5761 aacgcctgat ttatctggcg acgatttagt gcttcttcag tttgaatcag tgcaaagacg
     5821 acagttttct cgaagtctct tttcaacgga aataaaaaag tctggaaaat aacgtcctga
     5881 ttgaccgtta tagcgatcca gcttccccat cgtgcactgg gcctttcatt aatcgttaag
     5941 ttacccgtat agtcactttc tcatttatca ctaaaggctc ggtaaaaatc atggcc
//
LOCUS       DAJTNA010000214         5982 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTNA010000214 DAJTNA010000000
VERSION     DAJTNA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.217"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..207
                     /locus_tag="OO161_003663"
     CDS             <1..207
                     /locus_tag="OO161_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5464569.1"
                     /translation="YRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRA
                     IAGLNVEGLKKNARDISLLRYMPA"
     gene            complement(402..1493)
                     /locus_tag="OO161_003664"
                     /pseudo
     CDS             complement(402..1493)
                     /locus_tag="OO161_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1501..2068
                     /gene="parM/stbA"
                     /locus_tag="OO161_003665"
                     /pseudo
     CDS             1501..2068
                     /gene="parM/stbA"
                     /locus_tag="OO161_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
     gene            2071..2422
                     /locus_tag="OO161_003666"
                     /pseudo
     CDS             2071..2422
                     /locus_tag="OO161_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
     gene            2529..2666
                     /locus_tag="OO161_003667"
     CDS             2529..2666
                     /locus_tag="OO161_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464570.1"
                     /translation="MKLIHGAYEKAAPIIKIGEFAMSGNNVSDGLFASPERDIAASHE
                     R"
     gene            complement(2798..3073)
                     /locus_tag="OO161_003668"
     CDS             complement(2798..3073)
                     /locus_tag="OO161_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5464571.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(3073..3357)
                     /locus_tag="OO161_003669"
     CDS             complement(3073..3357)
                     /locus_tag="OO161_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5464572.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDHPLPVPR"
     gene            3881..4060
                     /locus_tag="OO161_003670"
                     /pseudo
     CDS             3881..4060
                     /locus_tag="OO161_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4110..4319
                     /locus_tag="OO161_003671"
                     /pseudo
     CDS             4110..4319
                     /locus_tag="OO161_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(4401..4502)
                     /locus_tag="OO161_003672"
                     /pseudo
     CDS             complement(4401..4502)
                     /locus_tag="OO161_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4591..5010
                     /locus_tag="OO161_003673"
     CDS             4591..5010
                     /locus_tag="OO161_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5464573.1"
                     /translation="MEQKALSAEPRRSFSNEFKLQMVKLASQPGASVARIVREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSDTGVELLPVEITPDEPKEPVAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGWGR"
     gene            5007..5357
                     /gene="tnpB"
                     /locus_tag="OO161_003674"
     CDS             5007..5357
                     /gene="tnpB"
                     /locus_tag="OO161_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5464574.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            5388..>5982
                     /locus_tag="OO161_003675"
     CDS             5388..>5982
                     /locus_tag="OO161_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5464575.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLA"
BASE COUNT         1555 a         1458 c         1502 g         1467 t
ORIGIN      
        1 taccgtgctc tggggatgga taaaccggaa ccagtggcga aagtgtgcta tgcacaaatg
       61 gtgaaacagt tcctgagtcg tgacccgttc gaatgcgtgc tgtgtggcgg ccggatggta
      121 taccgccggg ccatcgcggg actgaatgtg gaggggctga agaaaaacgc gcgggatatc
      181 agtctgctga ggtatatgcc ggcctgacac aggctaagtg tgcccgggaa tcggtgcaag
      241 tataaaaaac agcctgttac tgtataaaaa ccgaccatgc agagagagaa aacaccggag
      301 tggagggaaa agcagaaaag ctcacgtggc ataaggcgtg gacagcggta tcgtctggtt
      361 tttcaatttc ctatacgtga acacctgacg gcaggtatca ctcacccggt gaaggtaaat
      421 ccctcaagag gcagcaactc tgccagtcgc tcctccggcc attccggcag acgcgtcagg
      481 acgtccgtca gccaggcatg tggctccaga ccattacgtt ttgctgtttc cagaaggctc
      541 attatctgtg ccgcccggtt tccggccatc agtgaaccgg cgaacagcca cgctttccgt
      601 cccataacaa ccgggcgaat ggcacgctcg cagatattgt tgtccaaggg caccgctccg
      661 tcacccagga agcagctcag ttcatcgcgc cggttcagaa tatagttaat cgccttattt
      721 aatggcccgt cgggcggaca gcacggctcc tgctcttcaa gccaggcaaa caggtcattc
      781 actatcggcc gggagtaccg ttatcgccac tgccgtattt tttccacagg gcgctcacgg
      841 atgagtttct caatgcggta aagacccgcg atttttctca gctccacgcc tgcacggggc
      901 tccctgctgg ccttatacag atttgcgaag ccgcgccctg cgtgactcca gcaacaggct
      961 gatgtgatgc cggggtgatt atccgccaga gatttgtaca cgctgtatcc gtcactcacc
     1021 agcgtaccgc cacaccagca ctgcagccag gtctcagggt atctgagggc ccgtccggtc
     1081 tgcgagtcaa aacagacgac cggcggaccg cttctttcac cgctgacgta agcacacagc
     1141 cagccggagc aggatttacc gcctttccgg gtatcaagga gcctcaggga cgtttcatct
     1201 gcatgaataa catcccgtgt cagaagctca tggcgtagca gtttcgccag cggggccagt
     1261 gcagctccgg caacaccgac catatccgcc attgtactga cggggagctc cacacccatt
     1321 cgggagaaaa tgtgctgctg gcgatacagg ggcagatggt cggtatattt gctgatgaca
     1381 acctgtgcaa ttaccgacga ctcaacagca ctttcaggga ggatatgcgc cggcattttc
     1441 ccgctgaaca ctttctcaca gcagggacag ctgtactgcg gacggatata gcggattgca
     1501 ttaaatggtg gggatacatt cacaataaaa gatgtaaaag tcatgcctga atctataccg
     1561 gcaggttatg aagttctaca agaactggat gagttagatt ctttattaat tatagatctc
     1621 gggggcacca cattagatat ttctcaggta atggggaaat tatcagggat cagtaaaata
     1681 tacggagact catctcttgg tgtatctctg gtcacatctg cagtaaaaga taccctttct
     1741 cttgcgagaa caaaaggaag tagctatctt gctgacgata taattattca caaaaaagat
     1801 aacaactatc tgaagcaacg aattaatgat gagaacaaaa tatcaatagt caccgaagca
     1861 atgaatgaag cacttcgtaa acttgagcaa cgtgtattaa atacgctcaa tgaattttct
     1921 ggttatacgc atgttatggt tataggcggt ggcgcagaat taatatgcga tacagtaaaa
     1981 aaacacacag attcgtaatg aacgtttttt caaaaccaat aactctcaat atgatttagt
     2041 taacggtatg tatctcatag gtaattaata atggacaagc gcagaaccat tgccttcaaa
     2101 ctaaatccag atataaatca aacagataaa attgtttgtg atacactgga cagtatcccg
     2161 caaggggaac gcagccgcct taaccgggcc gcactgacgg cagatctggc cttatacaga
     2221 caagatcccc ggccccccat ttcttttatg tgaactgctg acgaaagaaa ccacattttc
     2281 ggatatcgtg aatatattga gatcgctatt tccaaaagag atggtcgatt ttaattcttc
     2341 aacaatcact caatcctctt cacaaaaaga gcaaaaaagt gatgaagaga cccaaaagaa
     2401 cgtgatgaag ctaataaact aattcaatta ttattgagtt tcctttatcc actatcaggc
     2461 tggataaagg aaacttaatc aagttatttt cttgccaatc attacataaa ataaacaggg
     2521 tcgttattat gaaattaatc cacggtgctt atgaaaaagc ggctccaata atcaaaattg
     2581 gtgagttcgc aatgtctggc aataacgttt ctgatgggtt atttgccagc ccggagcgcg
     2641 atattgccgc cagtcacgag cggtaatttt cgttatttca tcgtaattta ttttatctcc
     2701 tacaagatta tttaatggaa tgcaaaatat ctggcctgat aacaatgctc cagaattaat
     2761 acctgcaggc tatgttaaag ttgcctccac acccaatcta cctgttctct cgtgtgtgcc
     2821 acaggcgcaa tacataaata gtctggcctg taagttcgta acggatttcg tactcgccag
     2881 taaaaatccg tctgacctcc ctgggttcaa actgaaacaa ctgttctccc atacgtggat
     2941 gagttaacag aatgaccggc gcctgtgtca gggactgcac cgttctggcg gcagcaggtt
     3001 tactggccag caccagaaaa tcatataacc gcgccaaatc agaaagtgcc ttactggtcc
     3061 acttcagttc catcagcgtg gcaccggtaa cggatggtca gtactgaggc tgtccgccca
     3121 ggcctgtaca gcctgatggt cgataacctg tccggatgtc acatcgtcca gggcttccag
     3181 cgtcaggcga ttacgctcct cctcttgggc aagccatgca gaaagcgcct gtttgataac
     3241 ccagccccgg gagcgttcca gtctggcggc catctggtcg actttatcag ccataggtaa
     3301 tggcacatgc gcggtaatta cttttgttgc ttgtgcggta ttgtttttca tttgcatacc
     3361 cgattagttg ttaatcatat ttaatcatag tgattacttt cagacggaac aaggcaaata
     3421 atgcgagtca gcgcatataa ggcggcatac ggtggttaca tgtagcacag gtgtaaaacc
     3481 gccgaagccc ggcgcaagcc ggtacggtcg tccgaatatc accagtcccc ctccggctct
     3541 gcgaggccat gcggcctgtc agcgatgaaa atgcctctgt gcgcggtcgg agtggtcccg
     3601 acaagggttt acccgcagtc ggggcgtatc tccgcgttag cgggccgtga gggccgctta
     3661 cgagcgtgta tgagagacgt acagtagttt tgtccgctca caccagtaca aaccagtcga
     3721 aataccggta acgacgatta ctcttgtctc tgatgccggt ggactacgag attttattta
     3781 ctggcgtcct gatgcggtca ggactggtat ggaacctgtt tatgtgatac ttaatgctct
     3841 tctggattcc gggcgattta cgcgtaaact tattctgtta gggctgtcag gctcattttc
     3901 ctatatcttc ggctcaattg ttgcgacact gggaatggga cttgttgtcg attatctggg
     3961 atggggggcc acatttatcg tactgatact gtctgctgtt tttgccatca ttttcacact
     4021 gatgagtcgt gaacgctcac tggagttcga aaaagagtaa ttttttcagg aaaaacataa
     4081 aattactgac taaatattta ttttttgaat cgcagggcat tccggataag gttgatatcg
     4141 tgtcacggct gaagacctat ggatacagta tttctggtgt ggagacggat gacggatata
     4201 aggcgcttgt ccgcgctttt cagcttcatt tccggcagaa aaattatgac ggcataatgg
     4261 atgctgaaac cgctgccatc ctgtatgcat tgctggagaa gtatttcccg ggtaaataag
     4321 gagtgagtcg tcagagctgc gatatatccc cttcctccat gggctgtctc ttatacacaa
     4381 atccccaccc ggggatttgt gatgtcagat gtcccctgta agactatgtt ttcattgcgc
     4441 tctatgaagc tgagtgagcg taacgactgg agctgcttct gcggtgctcc ggtggcgaac
     4501 gtgagtaagc gtaaactgac cgccgtatgt agccattaag cctgtattgg taacgtaggt
     4561 gcccaccttt tcaactagtg gacacctgtt atggaacaga aagcattatc tgcagaaccc
     4621 cgcagatcat tttcaaatga gtttaaactt caaatggtta aactggcttc acaaccagga
     4681 gcctctgttg cccgtattgt ccgggaacac gatatcaatg ataacctgct gttcaaatgg
     4741 ctcaggctct ggcagaacga agggcgcata tcgcggcgtc ttccggtaac aacctcttct
     4801 gacactggcg ttgaattatt acctgtggag ataacgccgg atgagccgaa agaaccggtg
     4861 gctgctctta ctccgtcttt atctactcag actacagtta gtgccagctc ctgcaaggtg
     4921 gagttccgtc acggtaacat gacgctggaa aatccttcac cagagctgct cacagtgttg
     4981 atccgtgaac tgaccgggtg gggacgatga tctcactccc gtcaggcacc cgcatctggc
     5041 tcgttgctgg gataaccgat atgcgtaagt ctttcaacgg gctgggtgaa caggtacagc
     5101 atgtgctgaa tgataatccc ttctccggtc acctgttcat cttccgtggc cgacggggtg
     5161 acatgattaa aatcctgtgg gctgatgctg atggtctgtg cctgttcacc agacgcctgg
     5221 aggaaggcca gtttatctgg cctgctgtgc gtgacggcaa ggtatccatt acccgctcgc
     5281 aactggcaat gctcctcgat aagctggact ggcgtcagcc aaaaacatcc cgccttaacg
     5341 cactgacaat gttgtaaaaa tgtcatggcc ggattataaa aacggccatg aatcaaaaat
     5401 acctcattcg cattgcagaa ctggaatgcc agctccgtca gaaagaccag caactgagtc
     5461 tggttgaaga gacggaggcc ttcctgcgct ctgcactggc ccgcgccgaa gaaaagatcg
     5521 aagaagatga acgggaaata gaacatctgc gggctcagat agaaaaactg cgccggatgc
     5581 tgttcggtac ccgttctgaa aaactgcgtc gtgaagttga acaggctgag gccctgctga
     5641 aacaacgcga acaggacagt gatcgttaca gtgggcggga agacgatccg caggttcccc
     5701 gccagttgcg acagtctcgt catcgtcgcc cgttaccgga gcatctgccc cgcgaaataa
     5761 atcgcctgga gccagaagaa agctgttgcc cggagtgtgg cggtgagctg gattatctgg
     5821 gggaagtcag cgcagaacaa ctggaactgg tgagcagcgc tctgaaagtg atccgcacag
     5881 aacgggtaaa aaaagcctgt acaaaatgtg actgcatcgt tgaagcaccg gcaccatccc
     5941 gtccgataga gcgtggtatc gcgggcccgg ggttacttgc cc
//
LOCUS       DAJTNA010000215         5948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTNA010000215 DAJTNA010000000
VERSION     DAJTNA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.220"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..393)
                     /locus_tag="OO161_003676"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO161_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO161_003677"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO161_003677"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5464576.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEVISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGDMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO161_003678"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO161_003678"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5464577.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPSDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5948
                     /locus_tag="OO161_003679"
     CDS             5797..>5948
                     /locus_tag="OO161_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5464578.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCIL
                     ETLRVWV"
BASE COUNT         1831 a         1349 c         1001 g         1767 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt cactgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcttcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgac cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagtagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac cgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgta atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt cacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt gattcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tatagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg tagattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taacttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggaa tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctaaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcatcaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac gttttactga aaataataac tttcggaata tattctccac
     4561 acgaaactct agccgaaaaa tatagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaagcg
     4921 atgattactt ccccgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc cgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa atcatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcct tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac tcgtttttcc
     5821 cccgaagtcc gtcaacgggc agtccgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcga caatttgttc cattgctcca aagattggct gtatactgga gactctgcgt
     5941 gtctgggt
//
LOCUS       DAJTNA010000216         5942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTNA010000216 DAJTNA010000000
VERSION     DAJTNA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.221"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            146..1246
                     /locus_tag="OO161_003680"
     CDS             146..1246
                     /locus_tag="OO161_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5464579.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTLNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            1271..3666
                     /gene="torZ"
                     /locus_tag="OO161_003681"
                     /pseudo
     CDS             1271..3666
                     /gene="torZ"
                     /locus_tag="OO161_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(3864..4835)
                     /gene="cmoB"
                     /locus_tag="OO161_003682"
     CDS             complement(3864..4835)
                     /gene="cmoB"
                     /locus_tag="OO161_003682"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5464580.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLIRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4832..5575)
                     /gene="cmoA"
                     /locus_tag="OO161_003683"
     CDS             complement(4832..5575)
                     /gene="cmoA"
                     /locus_tag="OO161_003683"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5464581.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(5616..>5942)
                     /locus_tag="OO161_003684"
     CDS             complement(5616..>5942)
                     /locus_tag="OO161_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5464582.1"
                     /translation="LRLQYRVAYGDGGFSELQSAIRIHGNAVEYIPIAIVLMLFMEMN
                     GAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRSGMSATWCALLLMVLANLWYMPWE
                     LVFSLR"
BASE COUNT         1615 a         1419 c         1559 g         1349 t
ORIGIN      
        1 acccaattaa tgattacatt gtaataaatc atattcttta tcgattattt caggcagtgt
       61 tgtgccgagt tatgcaagcg gttaattcgt tgtgtattta attatccaat gatttcggtg
      121 ttctggaatt tatttagagg aatctatgcg agggaaaaaa cgcattgggt tattgttttt
      181 gctgatagcg gttgtggttg gtggcggcgg gttattgctg gcgcaaaaag ccttacataa
      241 aacgtcggat acagcatttt gcctttcctg ccactcgatg agtaaacctt ttgaggaata
      301 tcagggaact gtccactttt cgaaccagaa agggatacgt gcggaatgtg cagattgcca
      361 tattccaaag tcagggatgg attatttatt tgctaaatta aaagcatcta aagatattta
      421 tcatgaattt gttagcggca aaatagacag tgacgataag ttcgaaacac atcgccagga
      481 aatggccgaa acagtatgga aagaattaaa agcaactgac tctgcaacgt gccgtagttg
      541 ccattctttt gatgccatgg atattgcctc gcaaagtgaa tctgcgcaga aaatgcataa
      601 caaagcacaa aagggcggcg aaacctgtat cgattgtcat aaaggcattg cccattttcc
      661 gccagaaata aaaatggatg acaacgcggc gcatgagctg gaaagtcagg ccgctacttc
      721 agtgactaat ggcgcacata tttatccttt caaaacttct cgcataggcg agctggctac
      781 cgtgaatcct ggtaccgatc tcaccgtcgt tgatgccagt ggcaaacagc cgatcgttct
      841 gttgcagggt tatcaaatgc agggtagtga aaacacgctc tacctggcgg caggtcaacg
      901 gctggcgcta gccacattaa gtgaagaagg tatcaaggcg ctcacgctaa acggggaatg
      961 gcaggctgac gaatacggca atcaatggcg tcaggcgtct ttacagggtg cgcttaccga
     1021 tcccggatta gcggaccgta aaccgctatg gcaatacgct gaaaaacttg acgataccta
     1081 ttgcgctggt tgtcatgccc ctattgccgc cgaccattac accgtcaatg cgtggccgtc
     1141 cattgccaaa ggaatggggg cacgaaccag catgagcgaa aacgaactgg acattttaac
     1201 gcggtatttc cagtacaacg ccaaagatat taccgagaaa cagtgatcct cgttgatcag
     1261 ggaggaagtt atgacattaa caagacgtga atttattaaa cacagcggta ttgccgctgg
     1321 agcgctggtg gtgacatcgg cagcaccgct ccccgcatgg gcagaagaga agggcggtaa
     1381 aatcctcacc gcgggacgtt ggggagcgat gaacgtagaa gtgaaggacg gcaagattgt
     1441 ttcttcaaca ggcgcgctgg cgaaaaccat accgaattcc ttacagtcta cggcggcgga
     1501 tcaggtacac accacgacgc gtattcagca tccgatggtg agaaaaagct atctcgataa
     1561 tccactgcaa ccgacgaaag gtcgtggtga agatacctat gtacaggtga gctgggaaca
     1621 agcattaaaa ctgattcatg agcaacacga tcgcattcgc aaagctaacg gaccatcggc
     1681 tatttttgcc ggatcatacg gctggcgctc ttctggcgtg ttgcataaag cgcaaacctt
     1741 actgcaacgt tatatgaacc tggcgggcgg ttattccggg catagcggcg attattcaac
     1801 cggggcggca caggtgatca tgccgcatgt ggtcggttct gtagaggtgt atgaacagca
     1861 gacctcctgg ccgctgattc tggaaaacag ccaggttgtg gtgcgtgggg gatgaacccg
     1921 ctgaatacct taaaaattgc ctggagcagt accgatgagc aagggctgga atacttccat
     1981 cagctaaaaa aatctggcaa accagtgatt gccattgatc ctatccgctc cgaaaccatc
     2041 gacttctttg gcgataatgc cacctggatc gcgccgaata tgggcaccga cgtggcactg
     2101 atgttaggga ttgcgcatac cctgatgaca caaggcaaac acgataaagt gttccttgaa
     2161 aaatacacta ccgggtatcc gcagtttgaa gagtatctga gaggtaagag cgataatacg
     2221 ccgaagagcg cggcctgggc ggcggaaatt actggcgtac cggaagcgca gatagtcaaa
     2281 ctcgctgaac tgatggcggc gaatcgcaca atgctgatgg caggctgggg aattcagcgc
     2341 cagcaatatg gtgaacagaa acactggatg ctggtgacgc tggcggcggt gttagggcag
     2401 attggtacac cgggcggcgg ttttggtttc tcttatcatt actccaatgg tggcaacccg
     2461 acacgtgttg gcggcgtgct gccggaaatg tctgcggcga ttgccggaca ggccagcgag
     2521 gccgctgatg atggcggaat gagggcgatt cctgttgccc gtatcgtcga tgcgctggaa
     2581 aatcctggcg gcaaatatca gcataacggt aaagagcaaa cgtatccgaa tatcaaaatg
     2641 atctggtggg cgggtggcgg taactttacc catcaccagg atactaatcg tctgattaaa
     2701 gcatggcaga aaccggagat gatcgtcgtt tctgaatgct actggaccgc agctgctaaa
     2761 catgcagata tcgtattacc gatcaccaca tcgtttgagc gcaatgactt gacgatgacc
     2821 gttgattaca gcaaccagca tattgtgccg atgaagcagg ctgtcgctcc gcaatttgaa
     2881 gcgcgaaacg attttgatgt gtttgccgat cttgcggaat tactgaaacc tggcggaaaa
     2941 gagatctata ccgaaggtaa agatgaaatg gcgtggctga aatttttcta tgatgccgct
     3001 cagaaaggtg cccgtgcgca acgcgtgact atgccgatgt ttaatgcctt ctggcagcaa
     3061 aataaactga tcgaaatgcg ccgcagcgag aagaacgaac agtacgttcg ttatgctgat
     3121 ttccgcgccg atccgatgaa aaatgcgctg ggtacgccaa gcggcaaaat tgagatttac
     3181 tccaaaacgc tggaaaaatt tggctataag gattgcccgg cacacccaac ctggcttgcg
     3241 cctgatgagt ggaagggtac cgccgacgag aagcagttgc agcttctgac cgcacatccg
     3301 gcacaccgtt tacatagtca gcttaactat gcggaactgc gtaaaaaata tgcggttgca
     3361 gatcgtgaac caatcactat tcataccgaa gatgctgctc gctttggtat tgcgaatggt
     3421 gatctggtgc gtgtgtggaa caaacgtggt cagattctga caggcgcggt ggtgactgac
     3481 gggatcaaaa aaggcgtggt atgcgtgcat gaaggtgcat ggccagatct ggaaaatggc
     3541 ttgtgtaaaa acggcagtgc gaacgtgtta acggcggata tccccagctc gcagctggca
     3601 aatgcctgtg ccggtaactc tgcgctggtg tatatcgaaa aatatacggg taacgcaccg
     3661 aagtgaacgg catttgataa gcctgctgtt caggcgtaag aagtgaatag cgcccggaaa
     3721 cgggcgctga ggttatgcaa acgtaaaact gcctgatgcg ctatgcttat caggcctaca
     3781 tagtccctgc aatagattga attagcaagg gtttgtaggc cggataaggc attcacgctg
     3841 catccggcag taataccaga cctttacggc ttgcgcgcaa tcagcaccgc gcgtttaggc
     3901 gcaggataac cttcaactgt cttactcgga tcatgtgggt cgaggaaatc ggacagagat
     3961 tcggtaacca tccattcagt gcgtcgctgc tcttcagtag tggtaacgca cacatccaca
     4021 acgcggatat ctacaaaacc acacttcttc agccagtttt tcagcgccag cgcggaaggg
     4081 atgaaataga cattacgcat ttgcgcgtaa cgatcgcctg gtaccagcac cgtgttctca
     4141 tcgccatcaa taaccagcgt ttccagcacc agttcaccct cattcaccag ttgatctttc
     4201 aactgccaga gatgctccag cggcgaacgg cgatggtaaa gcacgcccat cgaaaagacg
     4261 gtatcaaagg ctttcagtgc cggaagctgt tcaataccta acggtaacaa atgtgcgcgc
     4321 tgatcgttac ccagtagttt acgcaccgct tcaaactggc acagaaacag ttgcgtaggg
     4381 tcgatcccca ccgcgaggtg cgcgcctgcg ccaatcatgc gccacatgtg ataaccgctg
     4441 ccgcagccga catccagaat ggtgcgtccg gttaaatcag aaagatgggg cagaacacga
     4501 tcccatttcc agtcggaacg ccattcggta tcgatgttga cgccatacag tgagaacggc
     4561 cctttgcgcc atggcatcag gttccgtata agcgtttcaa tgcgtttaat ttgcccggtg
     4621 ctcagtggct cttcgctttc ggcggttacg ctatgcaata aatccagacg atacggttta
     4681 atttcaggca gaaattccac cgcgttggac cactgcttaa acagcccgtg ctgctgctcg
     4741 cgctgccagt tagcaatctg cgcgggcagc gtttcgagcc agtgtgaaag atgatttttg
     4801 gcaatcagag aataaaagtt accaaagtcg atcatgcagc gtcctctgct tttaatgcca
     4861 ccagtgaacc aaagttaaag cactggaacc acagctcgct atgctcaaaa ccggctttat
     4921 gcaggcgtgc tttatgggtt tccacggaat cggtcagcat cacgttttcc agcatgctgc
     4981 gcttctggct gatctccagt tcgctgtaac cgttggcacg tttaaagtcg tggtgcatgt
     5041 tgaacaacag ttcaccaact ttggcatctt cgaaactgaa tttttccgaa agcaccagcg
     5101 cgccgcccgg gttcagtcct tgataaattt tatccagtaa tgcctggcgc tcggaaggtt
     5161 ccaggaattg cagggtaaaa ttcagcacca ccatcgatgc gttttcaatg gcgatatcgc
     5221 gaatatcacc ttcaatgacg tctactggcg taggggcttt ataggcgtca atatgacgac
     5281 ggcagcgttc aatcatcgcc ggggagttgt cgatggcaat aattttgcaa ttatcatgat
     5341 gaatgttgcg acgcaccgag agcgtcgccg cgcccagaga acaacccaga tcgtaaacct
     5401 gcgtaccagg ttgaacgaag cgctcggcta acatactaat catggaaata atattggaat
     5461 agccgggaac ggaacgctgg atcatatccg ggaagacttc agctacccgt tcatcaaagg
     5521 tccagtcgcc cagtctggcg ataggggcag aaaatagcgt gtcgcggtga gacataacgt
     5581 aaaaatccgg gaaaaagaaa gtggcgtatt gtgcgctaac gcagggagaa aaccaactcc
     5641 cagggcatat accaaagatt cgccagcacc atcagcaaca gcgcacacca ggtggcgctc
     5701 atgccggaac gtcgccagcg aaacagacgg tgatgaaaac cgtaataatg catcagacga
     5761 ccagcaagca aaacgatgcc gcaaatatgc accatccagg tttctgcgcc attcatttcc
     5821 ataaacagca tcagcacaat cgcgatggga atatattcca ccgcgttacc atgaatgcga
     5881 atagcgcttt gcagttcgct aaaaccaccg tcaccatagg caacgcggta ctgcaaacgc
     5941 aa
//
LOCUS       DAJTNA010000217         5907 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTNA010000217 DAJTNA010000000
VERSION     DAJTNA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.222"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..227
                     /gene="fadM"
                     /locus_tag="OO161_003685"
     CDS             <1..227
                     /gene="fadM"
                     /locus_tag="OO161_003685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5464583.1"
                     /translation="NYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(279..974)
                     /gene="queC"
                     /locus_tag="OO161_003686"
     CDS             complement(279..974)
                     /gene="queC"
                     /locus_tag="OO161_003686"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5464584.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1039..2739)
                     /locus_tag="OO161_003687"
     CDS             complement(1039..2739)
                     /locus_tag="OO161_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR5464585.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2839..3657
                     /gene="cof"
                     /locus_tag="OO161_003688"
     CDS             2839..3657
                     /gene="cof"
                     /locus_tag="OO161_003688"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5464586.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            3810..4268
                     /gene="decR"
                     /locus_tag="OO161_003689"
     CDS             3810..4268
                     /gene="decR"
                     /locus_tag="OO161_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5464587.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4298..>5907
                     /locus_tag="OO161_003690"
     CDS             4298..>5907
                     /locus_tag="OO161_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5464588.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEA"
BASE COUNT         1356 a         1465 c         1593 g         1493 t
ORIGIN      
        1 ttaactatcg tcgcccagcg gtattaagtg acctgttaac tattaccagt cagttgcagc
       61 aattaaacgg taaaagcggc atcttaagcc aggtcattac actggagccg gaagggcagg
      121 tggtagcgga tgcgcttatt acgtttgttt gtattgatct taaaacgcag aaagcattag
      181 ctctggaagg ggaattgcgc gaaaagctgg agcagatggt taagtaaacg ttttgtggtg
      241 ccggatgctc aagccgcatc cggcgacacc cggaataatt acctcaaccc ggttttctgc
      301 ttcatcgctg ccatcaccgt cggtttatcg gccagataat gattcaaacc gttggcgcgt
      361 aaattacatg ccgcacaatg accgcaaccg tcgcctttaa tgccgttata gcaggtcaac
      421 gtttcgttac ggactaaatc cagtttgccg taataatctg ccagcgccca ggtttccgct
      481 ttatcaatcc acatcagcgg cgtttcaaaa cgaatatctt tcgccatgcc caaactgacg
      541 gcatggttta gtgctttcac aaactcatcg cggcaatccg ggtagccgga gaaatccgtt
      601 tcacagacgc cggtaattac ggcttctgct tttacctgat acgcatatat tgccgccagc
      661 gtcaggaaca aaatattacg ccctgggaca aacgtattcg ggatgccatc ggcttcaggt
      721 tcataatcag gcaccggaat gctgtcacgt gtcaggctgc tgaccgccag ctcgttgagc
      781 agcgttacat caagaacctt atgcgcgcgt gctcccagtt tcagcgccag ttcgcgtgcc
      841 acgtcgattt ctgcgcgatg tcgctgaccg taatcgaacg tcacgcaatg gacttcatca
      901 tattgttgta atgcctgcac cagacaggtg gtggaatcct ggcctccact gaacacaacg
      961 acagcacgtt tcatagataa tcctgcttga acaataaaag cgttatggta acgcctgcga
     1021 ttaacccgga ccagctattc attgcgacgg cgcgggaagc caggcttcgg taaattcaaa
     1081 ccagccgcgc ggtgtcagtc gcacaccgtt cacgccggga ggggcactaa tgcgatagtg
     1141 atagttgaac agcggcgtca gcgtcgcgtc tgccattaac tggctaaaaa ccgctttcag
     1201 ggcattaaat cggttttcct catcaggcat tacttgcacc gcgtccagcg tcgattgcag
     1261 atgtgcatat gctggagcgt cgaaaacatg tggccatagt ggatcgcaac gtagccattg
     1321 ctccagagta tattccggtg cttcgccaat taatctgtcg cccatcatga ggtctgcgtg
     1381 tgccagtagg gtcgtgtcgt cccagttttt tgcgttatga aaaataattg tgagttcaca
     1441 gccttccgct gccagtgtcg cctgtaggcg ttctgccatg gtatgaagtt ctatcggtag
     1501 gtgataaacc agcgtcaagg ttttcggtag tttgacttca tccggtactt gccagtgcgg
     1561 aatagtccag cctggcagta atgcatgact ggcggtgatc aggttttctc cgacttctaa
     1621 cgtttgtaat aaaccggatt gatgaataat ggagatcact tttcgcgcct gccagaggga
     1681 gagtcgggga cttttgcgca acgttaaata gcaaaaacct aaactgatgc cactactgac
     1741 ctggctgacc cgttgcagct cctccggttt gccgatggtg atttgcacgg gatgccgaca
     1801 actggttccc aaatcttttt cgaaaagcgg cggagttatc cagtactcaa ccgctttaag
     1861 cagcggatga cgcaaatggt aataatcatg gctctccagg cgcaccagct ccgctgtgaa
     1921 ttgtgttaag cgaaaaggac cagtgccgat cagtgggaat tgcggatgcg ccagatggct
     1981 gcaatagctc gccagccggt gcgcaagcca gtaatcgggg cgatgtaaaa agaaggtcag
     2041 acactgcgga tgggtgactt caatacgctt cacgctaata aataattgat ccagtgctgg
     2101 cagttgtaac agcatcaata atcgctggtg taagtgtgag gcttttactg catcgccgtt
     2161 atgccagtgt agggttgaac gaagataaaa gtcccagcgt aacccgtcag tagaggtttc
     2221 ccagtgatgc gctaaatcgc caatcgggcg ctgagtatta ttatcgaagc gggtcaggcc
     2281 ggaaaatatc tgcccggcga gatgctgctc ggcacggccg ggcaaaaagc ctggttgtag
     2341 cggttcgagc gggcgatagt agggaatacg caacgtgggt gtatcgtttt gccattgtcc
     2401 gcccataaac ggctgtaaca gagtgcgtag ctcacctggg gccagttgcg ccagctccag
     2461 cacatcttgc tgctttccgg tttccagtgc ctgttccatc atcgcattgc gtagcgattc
     2521 cggcgtgacc agaaagcgta attgtccgcg ctttccgcgt cctgactgcg cctgccactc
     2581 cagccatccc gcctcctgtg cctgacgcaa cagcgtacga acatggcgtt cgctgcaaaa
     2641 acagcgttcg gccagttcgc tgacggtgac ggtttgcggc tttccggctg aaggttgcca
     2701 cagacgttga tactggttaa gacggttgag caatcgcata taaacccgga acaatattat
     2761 ttaactattc actattactt ccgtatatat caggtgatac tcaatctcca ttaaccgtgt
     2821 cacagagtgg agaaagaaat ggctcgtctg gcagcatttg atatggatgg cactttattg
     2881 atgcctgacc atcatttagg tgagaaaacc ctctctactt tggcgcgact gcgtgaacgc
     2941 gacattaccc tcacttttgc cacggggcgt catgcgctgg agatgcagca tattctcggg
     3001 gcgctatcgc tggatgcata tttgattacc ggcaacggaa cgcgcgtgca ttctctggaa
     3061 ggtgaacttt tacatcgtga tgatttacct gcggatgtcg cggagctggt gctgtatcag
     3121 caatgggata cccgagccag catgcatatc ttcaatgacg acggttggtt taccgggaaa
     3181 gagatccctg cgttgttgca ggcatttgtc tatagcggtt ttcgttatca gataatcgat
     3241 gtcaaaaaaa tgccactcgg cagcgtcacc aagatctgct tctgtggcga tcacgacgat
     3301 cttacacgct tgcagatcca gctatacgaa gcattgggcg agcgtgcaca tttgtgtttt
     3361 tccgccacgg attgcctcga agtgctgccg gtgggctgca ataaaggcgc tgcattgacg
     3421 gtgctgaccc aacatttagg tttatcgttg cgcgattgca tggcctttgg tgatgcgatg
     3481 aacgatcgcg aaatgttagg cagcgtcggt agcgggttta ttatgggcaa tgcgatgccg
     3541 caactgcgcg cggagctccc gcatttaccg gtgattggac attgccgaaa tcaggctgtc
     3601 tctcactatt tgacgcactg gctggactat ccacatctac cttattcccc cgaataatga
     3661 gatcccttcc agcaccgggc aattgcccgg ttttttttgc gttgaatttg tcattttgtg
     3721 ccgtggtgtt taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa
     3781 aagagaaaaa aattctctgt ggaagggcta tgttagataa aattgaccgt aagctgctgg
     3841 ccttactgca gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga
     3901 caaccacccc ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca
     3961 aagtcgccct gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa
     4021 aaacgcaaca tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc
     4081 cagaagtgct ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg
     4141 ttgccgacat gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc
     4201 tgtcggacgt cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca
     4261 tcgaataaat atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag
     4321 ctggtatttc cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat
     4381 cgcgatgctg caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga
     4441 acaacacttt actaccgggc agatcctgat gtggatcgcc accatggtgc tgattgccgt
     4501 tgtggtttat ttcctgcgtt acgtctggcg ggtattgctg tttggtgcgt cttatcaact
     4561 ggctgttgaa ctgcgtgaag attattaccg tcagctaagc cggcagcatc ctgagtttta
     4621 cctgcgtcat cgcacgggtg acctcatggc tcgtgcgacc aatgacgtcg atcgcgtcgt
     4681 gtttgcagcc ggagaagggg tgctgacgct ggtggattca ctggtgatgg gctgcgctgt
     4741 gttgattatg atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat
     4801 gccagtgatg gcgatcatga tcaagcgcaa cggcgatgcc ttgcatgaac gctttaagct
     4861 ggcacaggcg gcgttttcta gtcttaatga ccgcacccag gaaagcctca ccagtatccg
     4921 catgatcaaa gcctttggcc tggaagatcg ccagtcggcg ttatttgccg cggatgccga
     4981 agataccggc aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat
     5041 ctatatcgcg attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt
     5101 gcagggcagt ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat
     5161 ttggccaatg ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcata
     5221 cagccgtatt cgcgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt
     5281 gccggaaggg cgtggcgaac tggatgtaaa tattcaccag ttcacgtatc cgcagactga
     5341 ccatcctgcg ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg
     5401 cgggccgact ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt
     5461 cagcgagggg gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg
     5521 gcgtagccgc ctggcggtag ttagccagac accgttcctt ttttccgaca ccgtagcgaa
     5581 caacatcgcg ttgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt
     5641 agccagcgta catgacgata ttttgcgtct accgcaaggt tacgatacag aagtgggcga
     5701 gcgcggcgtg atgctttccg gtgggcaaaa acagcgtatc tccattgctc gagcgttatt
     5761 agtcaacgcg gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga
     5821 gcaccagatc ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc
     5881 ccatcgcctt tcggcactga cggaagc
//
LOCUS       DAJTNA010000218         5869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTNA010000218 DAJTNA010000000
VERSION     DAJTNA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.223"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..126
                     /locus_tag="OO161_003691"
     CDS             1..126
                     /locus_tag="OO161_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5464589.1"
                     /translation="MIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            215..1675
                     /locus_tag="OO161_003692"
     CDS             215..1675
                     /locus_tag="OO161_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5464590.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHCAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1711..1914)
                     /gene="ydfZ"
                     /locus_tag="OO161_003693"
     CDS             complement(1711..1914)
                     /gene="ydfZ"
                     /locus_tag="OO161_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5464591.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2092..2778)
                     /gene="ydfH"
                     /locus_tag="OO161_003694"
     CDS             complement(2092..2778)
                     /gene="ydfH"
                     /locus_tag="OO161_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5464592.1"
                     /translation="MTVETQLDPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSHQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIEYAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQISDCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2867..3613)
                     /gene="ydfG"
                     /locus_tag="OO161_003695"
     CDS             complement(2867..3613)
                     /gene="ydfG"
                     /locus_tag="OO161_003695"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5464593.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3750..5795
                     /gene="dcp"
                     /locus_tag="OO161_003696"
     CDS             3750..5795
                     /gene="dcp"
                     /locus_tag="OO161_003696"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5464594.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKLDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLHWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1478 a         1377 c         1524 g         1490 t
ORIGIN      
        1 gtgattggtt taatctctgc gctgaaaatg ccagaggtga aagaccgtga tttaagtgcg
       61 ctggaagacg ctgcggaaga tcaaccgcgt gttgtaagag ctgcgcaacc ttccagaagt
      121 ctgtaaaccc tcaatccctt ctcttaccgg agaggggatt tttattcata taacaaaaca
      181 tatagcttgc catatttaaa tttaaggaat tgttatggga aataatttgt tatcagcaaa
      241 agcgacactc cctgtttatg atcgtaataa cctagcccca agaattgttc atttaggctt
      301 tggtgcattt cactgtgcgc atcagggcgt gtatgccgat attcttgcta cagaacattt
      361 cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa ttgccgattt
      421 acaacagcaa gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt ggacggctcg
      481 cgtcgttggc gtcgttaaaa aagccttgca cgtacagatg gatggcttag aaaccgtgtt
      541 ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg aaaaagggta
      601 tttccactct ccggcgaccg gacagttaat gctcgatcac ccgatggtcg ctgccgacgt
      661 gcaaaatccc caccagccga aaacagcaac aggggtgatt gttgaggcgc tggcccgccg
      721 taaagcggca ggacttcccg catttaccgt catgtcatgt gacaacatgc cagaaaacgg
      781 tcatgttatg cgtgacgttg tcacttcata cgcgaaagct gttgatgtaa aactggcaca
      841 atggatcgaa gataacgtga ctttcccatc aacaatggtg gaccgtattg tgcccgcagt
      901 gacagaggat acgctggcga aaatcgaaca acttaccggt gtgcgcgatc ctgcgggcgt
      961 tgcctgtgaa cctttccgcc agtgggtaat agaagataac tttgttgccg gacgtccaga
     1021 atgggaaaaa gcgggagccg aactggttag tgatgtgctg ccttatgaag agatgaagtt
     1081 gcgcatgctc aacggcagtc attcattcct ggcgtatttg ggttatcttg ccggatatca
     1141 gcacattaat gactgcatgg aagatgaaca ttatcgttat gcggcgtatg gcttgatgtt
     1201 gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt acgctaaccg
     1261 attaattgaa cgctatagca atccggcgtt acgtcatcga acctggcaga ttgcgatgga
     1321 tggtagccag aaattgccac agcggatgtt ggattctgtt cgctggcatc tggcgcatga
     1381 cagcaagttc gatctgctgg cgctgggcgt cgcgggttgg atgcgttatg tcggtggtgt
     1441 tgatgaacag ggaaatccga tagaaatcag tgacccactg ttacctgtta ttcagaaggc
     1501 tgtacaaagt agtgccgaag ggaaagcgcg cgtccagtca ttgctggcga ttaaggcaat
     1561 ctttggtgat gatttgccag acaatagctt gtttactgca aaagtgacgg aagcgtactt
     1621 gtctttatta gcgcatggag cgaaagcgac cgtggcgaaa tattccgtga agtaaaaaaa
     1681 gcaatgccgc catcggcggc attcacacac ttagttcatg ccgagacgaa tcaggtccag
     1741 aggtgccagt ttctcttcac aaccttcaac aactacggtt tttccgcggc ggatttgctc
     1801 agcggtcaga ccttcagtat caatcgacag gatcttgcca gtgtgaccgg tgccgctaac
     1861 cataacacgg ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg tcataggtgt
     1921 tttctccttt ctgatttaca gtcgaagccc acgtaacggg cgcaatataa atactcctgt
     1981 cgagtgaaat attttttgtt tttgatcaag ctcacatctt ttttgatgca tgcatgagcc
     2041 gtaaggcggg gggaaaagcc tctcatcccg gatgggatga gagtaaatga attactcttc
     2101 gctaaaccag tcgctgtttt cctggcggat ctggcgcacg gattcgctga tttcctgcaa
     2161 atgttgcgtc attgcacgtt ctaccgcatc gccatcacgt ttttgcaggg cagagaaaat
     2221 atcaagatgc tggcgtaaca gcatttctgg tggagaaaca tggtcgaaac tcatatagcg
     2281 cacgcgatca acggtcgctt tcaggttctc aatggtatcc cacgccagtt gacagtcgga
     2341 aatctgcgtc aggagttgat ggaagttgtc atcaagttca aaaaaatcat ccagttgctt
     2401 gcgctcaatg gcaatgcgtt gctggtgaag attttgttcc agttggtagc actggctttc
     2461 ggtaatcatg ctcgccgccc gacgcgccac cgcgtactca atggcctgac ggataaaact
     2521 gccgttgcgc acctgggcca tggaaatttt gttgacgtag ctgccacgct gtggacgaat
     2581 ttgaatcagg ccgttttccg ccagtttaat aaaggcttca cgaaccggct ggtgtgacac
     2641 attgaaacga acagaaactt ctttttccga caacggtgtg cctggagcaa tcaggcaatg
     2701 gacaatgtcg cgacgaagga tacgataaat ttgctgattg acaggctgtg tgggatcaag
     2761 ttgcgtttcg acggtcattc gttcttgctt gtgagtgagt taactgcatg agtctaccac
     2821 ttttttgtga caagcaataa cccggcagtt atgccgggta taaaaattac tgacggtgga
     2881 cattcagtcc ggcatagctt tgggtaaccg gcatcatttc cagggtattg atattgacgt
     2941 gagcaggcag cgttgacacc caccaaacgg cttcgctgac atcttctggc gtcaatgcaa
     3001 cggtattttg ataggttttt tccgctttac cgtcatcgcc tttaaagcgg acattggaaa
     3061 actcggtgcc acccaccaga cccggttcga tgtcggtgac gcgcaccgcc gtaccatgca
     3121 ggtccgtacg cagattcagg ctaaactgac gaacaaacgc tttcgtcgca ccgtaaacgt
     3181 taccaccggc ataaggccag ctacctgccg ttgagccaat gttaataata tgaccatgat
     3241 tacgttcaac catacccggt aagacggcgc gcgtcatata taccaggcct ttgttgttgg
     3301 tatcaatcat cgtttcccag tcttcaacgc tggctttatg cgcaggctcc atgcccaacg
     3361 ccaggccggc attatttacc aggatatcaa tattgcacca ctcggcagga agcgatgcca
     3421 gcatctcttc aatagcggcg cggttgcgaa cgtccagttg ggcgatgtac agattatctc
     3481 ccagttcgtc ttttaactcc tgcaaccgtt cctggcgacg gccagtggcg ataactttat
     3541 gcccttgttg aataaaacga cgagtaatgc attcaccaaa acctgccgtt gctccagtta
     3601 ctaaaacgat catctcactg ttcctcaacg cttttgtgtg tcaataacat agcacgtgat
     3661 gcttaagagc gggtaatact ggtttgtaag attgcactcc gttatcagta agcctacgct
     3721 atggaacacc tcgcgttcag gagaagaaaa tgacaacaat gaatcctttc cttgtgcaaa
     3781 gcacactacc gtatctggct ccccattttg atcaaattgc caatcatcac tatcgcccgg
     3841 cattcgatga gggaatgcag caaaagcggg cagaaattgc tgccatcgcg cttaacccgc
     3901 aaacgcctga tttcaacaat actattctgg cactggaaca aagcggagaa ttacttaccc
     3961 gcgttaccag cgtctttttt gcgatgactg cggcgcatac caatgatgaa ttacagcgtc
     4021 ttgatgaaca gttttccgct gaactggcgg aactggctaa tgatatctat ctgaacggtg
     4081 aattattcgc gcgggtagat gctgtctggc agcgccgtga atccctgggg cttgatagtg
     4141 aatccatccg cctggtggag gtgattcatc aacgttttgt ccttgccgga gccaaacttg
     4201 cgcaagctga taaagcaaaa ttaaaagtac tgaatacaga agctgcgacc ctgaccagcc
     4261 agtttaacca gcgattactg gcagcaaata aatccggcgg tctggttgtg aacgatatcg
     4321 cgcagctggc aggaatgagt gagcaagaga ttgcgctggc ggcagaggcg gctcgcgaga
     4381 aaggtctgga taacaaatgg ctgattccgc tgctgaatac cacccaacaa ccggcgcttg
     4441 ccgagatgcg cgatcgtgcg acgcgtgaaa aactgtttat tgcgggctgg acgcgagcgg
     4501 aaaaaaatga tgccaatgat acccgcgcta tcattcaacg tctggtagag attcgcgcac
     4561 agcaggcgaa actgcttggt tttcctcatt atgccgcatg gaaaatcgcc gatcagatgg
     4621 caaaaacgcc agaagcagca ctcaacttta tgcgggaaat tgttccagcg gcgcgtcaac
     4681 gtgctagcga tgaattagcc tccatacagg cggttatcga taagcagcaa ggcgggttta
     4741 gcgcgcagcc gtgggactgg gcattttatg ccgaacaggt acggcgggag aaattggatc
     4801 ttgatgaggc gcagctcaag ccatattttg aattaaacac ggtgttgaat gaaggtgtat
     4861 tctggaccgc gaatcagctc ttcggtatta agtttgtcga acgttttgat attcctgtct
     4921 accatcctga cgttcgtgtg tgggaaattt ttgatcataa tggcgtggga ctggcgttat
     4981 tttacggtga tttcttcgcc cgtgattcaa aaagcggcgg tgcatggatg ggcaattttg
     5041 ttgagcaatc aacgcttaat gaaacgcatc cggtaattta taacgtttgc aattatcaga
     5101 aacccgctgc cggtgagcct gcgttgttac actgggatga tgtcataacc ttattccatg
     5161 aatttggtca tacgctgcac ggcctttttg cccgccagcg ttatgccacg ctttccggca
     5221 ccaacacgcc gcgtgatttt gttgaatttc cgtcgcaaat caacgaacac tgggcaacgc
     5281 atccgcaggt attcgctcgc tacgcccggc attatcagag cggggcagca atgcctgacg
     5341 aactgcaaca gaaaatgcgt aatgccagcc tgttcaacaa agggtatgag atgagcgaac
     5401 tgcttagcgc cgcacttctc gatatgcgct ggcattgcct ggaagaaaac gaagcaatgc
     5461 aggatgtcga tgattttgaa ttgcgggcgc tggtggcgga aaatatggat cttcctgcta
     5521 taccgccacg ctatcgcagc agttatttcg cccatatttt tggtggcgga tatgctgcag
     5581 gttattacgc ttatctgtgg acgcaaatgt tggccgatga tggttaccag tggtttgttg
     5641 agcagggcgg attaacgcgt gaaaatgggc agcgttttcg cgaggcgatc ctttccagag
     5701 gtaacagcga agatctggaa cgcctgtatc gacaatggcg cggtaaggct cctcagatta
     5761 tgccgatgct gcaacatcgt ggcttgaata tataaacacc accaatggct ggcctggaga
     5821 tatcgccagg ccagcctggt tatagcagta attgcagcag ctggacgag
//
LOCUS       DAJTNA010000219         5799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTNA010000219 DAJTNA010000000
VERSION     DAJTNA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.224"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..122
                     /locus_tag="OO161_003697"
     CDS             <1..122
                     /locus_tag="OO161_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5464595.1"
                     /translation="GTVNKSKVSLGLSATYGQTGNKVAAGTVQSVIGVTFIYE"
     gene            456..2735
                     /gene="ydeP"
                     /locus_tag="OO161_003698"
     CDS             456..2735
                     /gene="ydeP"
                     /locus_tag="OO161_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5464596.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2983..3181
                     /gene="safA"
                     /locus_tag="OO161_003699"
                     /pseudo
     CDS             2983..3181
                     /gene="safA"
                     /locus_tag="OO161_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
     gene            3256..4017
                     /gene="ydeO"
                     /locus_tag="OO161_003700"
     CDS             3256..4017
                     /gene="ydeO"
                     /locus_tag="OO161_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5464597.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4419..>5799
                     /locus_tag="OO161_003701"
     CDS             4419..>5799
                     /locus_tag="OO161_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5464598.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHK"
BASE COUNT         1677 a         1261 c         1262 g         1599 t
ORIGIN      
        1 tagggacagt aaataaaagc aaagtatctc tgggtttaag cgcaacctat ggacaaacag
       61 gaaacaaggt tgcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
      121 aattgtgatt ttgatcttag tctatgcgct ccattcacga aaattgcgcg gataattatt
      181 ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa gccagagtct
      241 tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg cttaacttat
      301 cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt agtcccctac
      361 tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat cgctattaca
      421 aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt gaatcctacc
      481 agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga
      541 tggatatacg ccaggatgtt attgccatgt ttgacatgaa taagccagag ggctttgact
      601 gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg
      661 gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct ttctttgctg
      721 agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct gcgggacgtc
      781 tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta agctggcaac
      841 aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat caggttgaat
      901 tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt tttgcccgtg
      961 aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg accagtgtgg
     1021 gtttggcagc gagtatcggc gtgggtaaag ggactgtgtt gctggaagac tttgagaagt
     1081 gcgatttagt catttgcatt gggcataacc ctggtacaaa ccacccccgc atgctgactt
     1141 cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct ctacaggaac
     1201 gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg aactctgaga
     1261 ctcagttggc cagtgcgtac tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg
     1321 ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt cggccctcat
     1381 tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta cgccgtgacg
     1441 ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag acacaaatcg
     1501 ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac ggaatgggga
     1561 tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg ctgttgatga
     1621 aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac tctaatgtac
     1681 agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg gctcgcctgg
     1741 gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt gccagcatgc
     1801 aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac tttgcactgg
     1861 caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg gcggtacacg
     1921 tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat attctgccgg
     1981 ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta accgttgagg
     2041 attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga
     2101 aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccag agcgtggtag
     2161 cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa gctgtgttac
     2221 cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac ctgataaatg
     2281 cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt accagcaaag
     2341 ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg acagtacgca
     2401 gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga ggggtattcg
     2461 gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg
     2521 gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg
     2581 atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc tattttccag
     2641 aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt cctggttata
     2701 aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt cttctgctgt
     2761 catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct cacattgatc
     2821 aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga aactccgctc
     2881 agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta agcatactga
     2941 ttaacgattt ttaacgttat ccgctaaata aacatatttg aaatgcatgc gaccacagtg
     3001 aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat tgtggttgtc
     3061 ttattactga cccttacgtt gatagccatt tttttcggca attgatcagc tgggtatttc
     3121 agaaatgggt cgcattgcaa gagatcttac acatttcatt atcaatagtt tgcaagactg
     3181 aaaataaaca gcaaattata aatatgaaat gttaaaaaag tatcgataaa aactttattg
     3241 ttttaaggag ataaaatgtc gctcgtttgt tctgttatat ttattcatca tgccttcaac
     3301 gctaacattt tagataaaga ttacgccttc tctgacggcg agatcctgat ggtagataac
     3361 gctgttcgta cgcattttga accttatgag cggcatttta aagagatcgg atttactgaa
     3421 aataccatta aaaaatatct acaatgcact aacatccaga cagtgacggt gcctgttcct
     3481 gcgaagtttt tacgtgcttc aaatgtaccg actggattgc ttaatgaaat gattgcttat
     3541 ctcaactcgg aagaacgcaa tcatcataat ttctcagaac ttttgctttt ttcttgcctg
     3601 tctatttttg ccgcatgcaa aggtttcatt acactattaa ctaacggtgt gctatccgtt
     3661 tccggaaaag tgagaaatat tgtcaacatg aagctggcgc acccatggaa gctgaaagat
     3721 atttgtgact gcctgtatat cagtgaaagc ctgttgaaga aaaaactaaa gcaagagcaa
     3781 acgacattct cacagattct attagatgcc agaatgcagc acgcaaaaaa tttgatacgc
     3841 gtagaaggtt cagtcaataa aattgccgaa caatgtggtt atgccagtac ttcttatttt
     3901 atttatgcgt tccgcaaaca tttcggcaac agtccgaaga gagtttctaa ggagtaccgt
     3961 tgtcaaagtc acacgggtat gaatacgggc aacacgatga gtgctttagc tatttgatta
     4021 tttgctaacg agtagtcaac cacgcacgct acgtaagaat tatatggggc agccattccc
     4081 tgccccgcgt tgtttttagg cgatatattt attgaaataa ataagtgaca tccatcacat
     4141 atttatgcac ttgcataacc tgttgcatga ttatttatga tctcaattca gcattttgtc
     4201 agtaaaatgc aataatttat taaatatcaa taaattagtt gtttatcggc gagaaattac
     4261 ttaatagaac agaaagtaat gtcaacgctt tatggactgt tatttccctt tttttagcta
     4321 aatctgctat ctctttatgt gactaacttc acttacatcc acttatttct cttcgtaaaa
     4381 ttacttcgga attaagtaca ataagaagag gaacatttat gaagtctgca ttaaagaaaa
     4441 gtgtcgtaag tacctcgata tctttgatac tggcatctgg tatggctgca tttgctgctc
     4501 atgcggcaga tgatgtaaag ctgaaagcaa ccaaaacaaa cgttgctttc tcagacttta
     4561 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
     4621 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
     4681 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
     4741 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
     4801 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
     4861 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
     4921 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
     4981 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
     5041 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
     5101 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
     5161 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
     5221 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctgc
     5281 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
     5341 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
     5401 aactgaagaa aaacggacag tatgataata ctattattct ctttacctcc gataatggtg
     5461 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
     5521 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     5581 attatgacaa gctgatttcc gcaatggatt tctacccgac agctcttgat gcagccgata
     5641 tcagcattcc aaaagacctt aagctggatg gcgtttcctt gctgccctgg ttgcaagata
     5701 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     5761 acgaggaaaa tattccattc tgggataatt accacaaat
//
LOCUS       DAJTNA010000220         5782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTNA010000220 DAJTNA010000000
VERSION     DAJTNA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.225"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..3023
                     /gene="ygfK"
                     /locus_tag="OO161_003702"
     CDS             <1..3023
                     /gene="ygfK"
                     /locus_tag="OO161_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCR5464599.1"
                     /translation="IFGIPEQQFYSPVKGKTVSVFGETCATPVGPAAGPHTQLAQNIV
                     TSWLTGGRFIELKTVQILDRLELEKPCIDAEDECFNTEWSTEFTLLKAWDEYLKAWFA
                     LHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQPPMQQFIDNMMDASDHPKFAQYRDTL
                     NKLLQDDAFLARHGLQEKRESLQALPARIPTSMVQGVTLSTMHGCPPHEIEAICRYML
                     EEKGLNTFVKLNPTLLGYARVREILDICGFGYIGLKEESFDHDLKLTQALEMLERLMA
                     LAKEKSLGFGVKLTNTLGTINNKGALPGEEMYMSGRALFPLSINVAAVLSRAFDGKLL
                     ISYSGGASQLTIRDIFDTGIRPITMATDLLKPGGYLRLSACMRELEGSDAWGLDHVDV
                     ERLNRLAADALTMEYTQKHWKPEERIEVAEDLPLTDCYVAPCVTACAIKQDIPEYIRL
                     LGEHRYADALELIYQRNALPAITGHICDHQCQYNCTRLDYDSALNIRELKKVALEKGW
                     DEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAGYFLARAGHPVTLFEREANAGGVVKNI
                     IPQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHYVLIATGTDKNSGV
                     KLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCARAALRVPGVEKATI
                     VYRRSLQEMPAWREEYEEALHDGVEFRFLNNPECFDADGTLTLRVMSLGEPDEKGRRR
                     PVETNEIVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDHNGETRLTDVFMIG
                     DVQRGPSSIVAAVGTARRATDAILSRENIRSHQNNKYWNNVNPAEIYQRKGDISITLM
                     NSDDRDAFVAQEAARCLECNYVCSKCVDVCPNRANVSIAVPGFQNRFQTLHLDAYCNE
                     CGNCAQFCPWNCKPYKDKITVFSLAQDFDNSSNPGFLVEDCRVRVRLNNQSWVLNIDS
                     EGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            3026..4360
                     /gene="ssnA"
                     /locus_tag="OO161_003703"
     CDS             3026..4360
                     /gene="ssnA"
                     /locus_tag="OO161_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCR5464600.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIAIENDVIVAIGDALTQRYP
                     DASYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDE
                     ESLYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTD
                     RNNGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREA
                     VKATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIA
                     LLNQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDT
                     GGPLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGH
                     IAFGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            4411..5190
                     /locus_tag="OO161_003704"
     CDS             4411..5190
                     /locus_tag="OO161_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCR5464601.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYPACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAEEEAV"
     gene            5187..>5782
                     /locus_tag="OO161_003705"
     CDS             5187..>5782
                     /locus_tag="OO161_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464602.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTA"
BASE COUNT         1429 a         1523 c         1494 g         1336 t
ORIGIN      
        1 caatctttgg tattcccgag caacagtttt actcacctgt aaaaggtaaa actgttagcg
       61 tcttcggtga aacctgtgcc actcccgtcg gccctgccgc tggcccacac acgcagctcg
      121 cgcaaaatat tgtcacttcc tggctgactg gcggacgttt catcgaacta aaaaccgtcc
      181 aaattcttga ccgcctggag ctggaaaagc cctgtattga tgccgaagac gagtgcttta
      241 acaccgaatg gtctaccgaa tttaccctgc ttaaagcctg ggatgaatac ctcaaagcct
      301 ggtttgccct gcaccttctc gaagcgatgt tccaaccttc tgattccggt aaatcgttca
      361 tctttaatat gagcgtcggt tacaacctcg aaggtattaa gcaaccgcca atgcaacagt
      421 tcatcgacaa tatgatggac gcatctgacc atccgaaatt cgcgcaatat cgcgatacgc
      481 tgaataaatt actccaggat gacgcatttt tagcccgcca cggattgcag gaaaaacgcg
      541 aaagcttgca agccttaccc gctcgcatcc ccaccagtat ggtgcagggc gtcaccctct
      601 ccaccatgca cggctgtcct ccgcatgaaa tcgaagccat ttgccgctac atgctggaag
      661 aaaaagggct caacaccttt gtgaaactca acccgacatt actggggtac gcgcgtgttc
      721 gtgagatcct cgatatctgc ggtttcggtt acataggctt aaaagaagag tcatttgatc
      781 acgacctcaa gctgacgcaa gcgctggaaa tgctggaacg cctgatggca ctggcaaaag
      841 aaaaatcact cggctttggc gtaaaactga cgaacactct cggcaccatc aacaataaag
      901 gcgcactgcc tggtgaagag atgtatatgt caggccgtgc gctgttcccg ctctccatca
      961 atgttgcagc agttctctct cgcgcctttg acggcaaact gctcatttct tattccggtg
     1021 gtgccagtca gctgactatc cgcgatattt ttgatactgg tattcgccct attactatgg
     1081 caaccgatct gctgaaacct ggcggctatc tgcgcttaag tgcctgcatg cgcgagctgg
     1141 aaggctccga cgcctgggga cttgaccatg ttgacgtcga acgactgaac cgactggcag
     1201 cagacgcgtt aaccatggaa tacacccaga aacactggaa gccagaagag cgtattgaag
     1261 tggcagaaga cctgccgctg accgattgct acgttgcccc ctgtgttact gcctgcgcta
     1321 tcaagcaaga tattccggaa tacatccgtc tgcttggcga acaccgctat gccgacgcgc
     1381 tggaactcat ctaccaacgc aacgctctgc ccgccattac cggtcatatt tgcgatcacc
     1441 agtgccaata caactgtacc cgcctggatt acgacagtgc gctgaatatc cgcgaactga
     1501 aaaaagtcgc gctggaaaaa ggttgggatg aatataagca acgctggcac aaaccagccg
     1561 gttctggttc acgccatccg gttgccgtga ttggtgcagg tccggcgggt ctggcagcag
     1621 gttacttcct tgccagagcg ggccatccgg ttacgctgtt tgaacgcgaa gccaatgcgg
     1681 gcggcgtggt gaaaaatatc attcctcagt tccgtattcc tgcagagtta attcagcacg
     1741 atatcgattt tgttgccgct cacggcgtga aatttgagta tggctgctca cccgatttga
     1801 ccgttgaaca gttaaaaaat cagggcttcc actatgttct gattgccacc ggcactgata
     1861 aaaatagcgg tgtgaaactg gcgggcgaca accaaaatgt ctggaaatca ctccccttcc
     1921 tgcgtgaata caacaagggt acagcgctca agctgggcaa acatgtggtc gttgtcgggg
     1981 cgggtaacac cgcaatggac tgcgctcgtg cggcgttacg cgttccaggc gtagaaaaag
     2041 caacgatcgt ttaccgtcgt tcactgcaag agatgcccgc atggcgcgaa gagtatgaag
     2101 aagcgttgca cgacggcgtg gagttccgtt tcctgaataa tccggaatgt ttcgatgctg
     2161 atggcacctt aaccttgcgc gttatgtcgc ttggcgaacc cgatgagaaa ggtcgtcgcc
     2221 gtccggttga aaccaacgaa atagtaacac tgcatgtaga cagtctgatc accgccatcg
     2281 gtgaacagca ggatactgaa gccctgaatg cgatgggcgt accgctggac aaaaacggct
     2341 ggccagacgt cgaccataat ggcgaaactc gtctgactga cgtctttatg atcggcgacg
     2401 tacagcgcgg accatcctcc attgtcgctg ctgtcggaac cgcgcgtcgg gcgaccgatg
     2461 ccatccttag tcgggaaaat atccgttccc accagaacaa taaatactgg aacaacgtca
     2521 atccggcgga aatctatcaa cgtaaaggcg atatctctat cacgctgatg aacagtgacg
     2581 atcgtgatgc gtttgtcgcg caggaagctg ctcgctgcct tgaatgtaac tacgtttgca
     2641 gcaagtgcgt ggatgtctgc ccgaaccgcg ccaacgtctc cattgcagtc cctggcttcc
     2701 agaatagatt ccagacgctg cacctcgacg cttactgtaa cgaatgcggc aactgcgctc
     2761 agttctgtcc gtggaactgt aaaccataca aagacaaaat caccgtcttc agcctggcgc
     2821 aagactttga taacagcagc aacccaggct tccttgtgga agattgccgg gtacgagtac
     2881 gtctgaataa ccaaagctgg gtgttaaaca tcgacagcga aggtcagttt aacaacgtac
     2941 caccggagct gaacgatatg tgccgcatca tcagccatgt ccaccagcat catcattatc
     3001 tgctgggccg cgtggaggtg taatcatgtt gattctgaag aatgtcaccg cagtgcagtt
     3061 acacccggcg aaagtgcagg aaggcgttga tatcgctatc gctatcgaaa atgacgtgat
     3121 tgtcgctatc ggcgatgccc tgacgcaacg ctaccccgat gccagctaca aagagatgca
     3181 tggtcggatt gtgatgccgg gaattgtctg ctcgcacaac catttttact cggggctttc
     3241 ccgcggaatt atggcaaaca tcgccccctg cccggatttc atctcaacgc tgaaaaatct
     3301 ctggtggcgg ctcgatcgcg cccttgatga agagtcgctc tattacagcg gactgatttg
     3361 ttccctggaa gcgattaaga gcggatgtac atcggttatc gatcaccatg cctctccggc
     3421 gtatatcggc gggtcgctct ccacattgcg cgacgcattt ttaaaagttg gcctgcgcgc
     3481 aatgacctgt tttgaaacta ctgaccgtaa caacggcatc aaagagttgc aggaaggtgt
     3541 agaagaaaac atccgtttcg cccgtttgat tgatgaggcg aagaaagcga caagcgagcc
     3601 gtatctggtg gaagcacata tcggtgctca cgcgccgttt accgtgccgg atgccggtct
     3661 ggagatgctg cgtgaagccg tgaaagccac aggccgtggt ttgcatattc acgctgcgga
     3721 agacctttac gacgtttcct acagtcacca ctggtacggc aaagacctgc tggcacgact
     3781 ggcgcaattc gatctcatcg acagcaaaac gctggtcgct catgggctgt acttgtcgaa
     3841 agatgacatc gccctactca atcagcgcga tgcgttcctg gtgcataacg cccgttcaaa
     3901 catgaacaac catgtcggct acaaccatca ccttagcgac atccgcaacc tggcattggg
     3961 aacggacggc attggttcgg acatgtttga agagatgaaa tttgccttct ttaaacatcg
     4021 cgatacgggt ggtccgctgt ggcctgacag ttttgccaaa gccctgacta acggtaacga
     4081 actgatgagc cgcaactttg gcgcgaaatt tggacttctg gaagccggtt acaaagctga
     4141 tttaaccatt tgcgattaca actcaccgac gccgctgctg gcagacaata tcgccgggca
     4201 tatcgctttc ggtatgggct caggcagcgt tcacagcgtg atggtcaatg gtgtgatggt
     4261 ctatgaagac cgtcagttta acttcgattg cgattccatt tatgcacaag ccagaaaagc
     4321 cgctgccagt atgtggcgtc ggatggatgc gctggcataa atgacagatg ccctcttccc
     4381 gcagggaaga gggttaaata aggaaagaag atgattgaac aatttttcag gcccgactct
     4441 gtcgaacagg cgctggaact gaagcgccgc taccaggatg aagccgtctg gttcgccggg
     4501 ggcagcaaac tcaatgctac accaacccgt accgataaaa agattgccat ttccttacag
     4561 gatctggaac tggactgggt tgactgggat aacggtgcac tgcggattgg cgcaatgtct
     4621 cgcttgcagc cactgcgtga tgcgcgattt attcctgcag cgctgcgtga agccctcggt
     4681 tttgtttact cacgccatgt tcgtaatcag tcgaccattg gtggtgaaat cgccgcccgg
     4741 caggaagagt cggtgctgct tcccgtcctg ttggcactgg atgctgaact ggtttttggc
     4801 aacggcgaaa cgctgtcaat cgaggactac ccggcctgcc catgcgatcg cctgctaacc
     4861 gaaattatca ttaaagatcc gtatcgcacc tgtgcgaccc gcaaaattag ccgttctcag
     4921 gcaggtttaa ccgtcgtgac ggcagccgtt gcaatgacag accacgacgg tatgcgaatt
     4981 gcgctggatg gcgtcgccag taaagcactg cgtctgcatg atgtcgaaaa acaaaatctg
     5041 gaaggcaatg cacttgaaca ggctgtcgcc aacgccattt tcccgcagga agatttgcgg
     5101 ggcagcgtgg cctataaacg ctatatcacg ggagttctgg tagccgacct gtatgccgac
     5161 tgccaacagg ctgaggagga agccgtatga tcatccactt tactttaaat ggcgcgcctc
     5221 aggagctaac cgttaatcca ggcgaaaacg tgcaaaagct gttgtttaac atgggaatgc
     5281 actctgtacg caacagtgat gatggcttcg ggtttgctgg ttctgacgca ataatcttta
     5341 acggtaatat cgttaacgcg tccttgctta ttgccgcaca gttagagaag gcagatattc
     5401 gtaccgcaga atctctgggc aaatggaacg agttaagtct ggttcaacag gcaatggttg
     5461 atgttggcgt ggtgcagtct ggttataacg atccagctgc agctctgatt atcaccgatc
     5521 ttctcgatcg catcgccgca cctacccgcg aagagatcga cgacgcgctt tctggtttgt
     5581 tcagccgcga tgctggctgg cagcaatact atcaggtcat tgaactggcg gttgcacgta
     5641 aaaataatcc gcaggccacc attgatatcg ctccgacttt ccgtgacgac ctagaagtca
     5701 ttggtaagca ttatcctaaa actgatgccg cgaaaatggt gcaggcgaaa ccctgctatg
     5761 ttgaagaccg cgtaacggct ga
//
LOCUS       DAJTNA010000221         5756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTNA010000221 DAJTNA010000000
VERSION     DAJTNA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.226"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..751
                     /gene="tam"
                     /locus_tag="OO161_003706"
     CDS             <1..751
                     /gene="tam"
                     /locus_tag="OO161_003706"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5464603.1"
                     /translation="WNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTA
                     LLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQW
                     LPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVH
                     AYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRY
                     HQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(755..1669)
                     /locus_tag="OO161_003707"
     CDS             complement(755..1669)
                     /locus_tag="OO161_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5464604.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSEILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(1866..2774)
                     /locus_tag="OO161_003708"
     CDS             complement(1866..2774)
                     /locus_tag="OO161_003708"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: protein motif:HMM:NF016846.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5464605.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGNVTSVTLPKLTRNTDSYRGGEVA
                     KLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKPYKERKVA
                     ILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIDEEIIQVLDLPRDELESFAS
                     AVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFALTALIAF
                     YRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTPELVAIVLAEKDHWEQDLTQVP
                     GLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(2774..3958)
                     /locus_tag="OO161_003709"
     CDS             complement(2774..3958)
                     /locus_tag="OO161_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCR5464606.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYMGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWNKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGVRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            4116..5225
                     /locus_tag="OO161_003710"
                     /pseudo
     CDS             4116..5225
                     /locus_tag="OO161_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
BASE COUNT         1504 a         1471 c         1424 g         1357 t
ORIGIN      
        1 ctggaacccc tctttatatt tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
       61 gcttgccaga gtgccgctgg aaaatgtcga atatgtcgcc gatcttggct gtggcccagg
      121 taacagcacc gcccttctac aacaacgttg gcctgcggcc aggataacag gcatcgactc
      181 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
      241 agacatccgc aactggcaac cggtacaggc actcgatctg atttttgcta atgcctcact
      301 gcaatggctg cctgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
      361 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
      421 cgaagttgcc tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
      481 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccaccta
      541 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
      601 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttaagc gctaccatca
      661 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
      721 tctttttatt gttgcccggc gtatggagta aatatcacgt cagctggtaa tgacgatcgg
      781 gaagaatttt tgctggaatt tcggcttcat cgttcatctg taagaggtca atttcaatag
      841 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
      901 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
      961 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacag
     1021 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt ataggcaatg cgctcgcatc
     1081 ctgccaggac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatttctg
     1141 aaagctgccc attgcggcgc tgaaccgcca accactcccc cattattaac aagatacggt
     1201 tagctggaga gttcgaagcc agtacaggct gaagatcttc agtcttgaga taatgagcca
     1261 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     1321 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctcacgta
     1381 gtaacgaccg tgaggcaatc atcaactgtc cccaaagttt tcgcgcttca acgtaacggg
     1441 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     1501 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aaaataacag
     1561 cgatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     1621 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     1681 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     1741 aaattaccta caagcttaca acattaacag gcgctgcatg tggcacctgt taatgataaa
     1801 ggcatatagg atgttgtaac taatatagtg aaataagaaa cccggtcgaa accgggttca
     1861 gagtattagc acaacgggcg tacagcttcg cgcatccctt tttcaagaat cgcatccagg
     1921 tcattagcaa cctgctcgac cagaccaggc acttgcgtca ggtcctgctc ccagtggtct
     1981 ttttctgcca aaacaatcgc taccagttcc ggggtgccga taacacggtc acgatgctgg
     2041 ctccagagtt gctgataacg ttccagccag tgtgcatcgt cctgtaccgg atatgtttct
     2101 ccgttacgct caccgcgata gaaggcaatc aatgccgtta atgcgaaagt aaggcgcgcc
     2161 ggaagtgtgc cgtttgcctt ctgccctgcc agcagctgcg gcaggatgcg ggtgcggaac
     2221 ttggtcatac cgttgagcgc gatagacagc agctgatgct taatgtacgg gttacggaaa
     2281 cgcccggtga ctgcactggc gaaagattcc agttcatcac gaggcaaatc cagtacctga
     2341 ataatttctt cgtcgatggc tttttcaacg aatgcgcaaa tttcagcatc gttcatcgct
     2401 tcacctacgg tatccagccc tgcctggaac gccaccggca ccagcgcggt gtgcgcaccg
     2461 ttgaggattg ccactttgcg ttctttatac ggtttaatat cgtcaacaat cagcacattg
     2521 agcggatatt tgtccaggcg cagctcagtc gctaaggatt tcggcccctg aatcacaaac
     2581 aggtaaaagt gttcagcggt gtcgagaaaa ccatcgtgat aacccagttc ttcttccagt
     2641 ttagccactt caccaccccg gtagctgtcg gtgttgcgcg tcagcttagg cagagtgacg
     2701 gacgtcacat tacccacttc gttttcacca tccacaaaca gcgtaaaaaa gcgaagatgt
     2761 tttggcacag ccatcaggca cctcccagca ccgcaaatgc gggttcaaag tattcatcag
     2821 taaacgtctg gtaaagctcc atgtcttcca gtggcggaac gggcgtatat ttgtagcgaa
     2881 tacgcacacg tccctgacgt aaatccgtgg tgctgttatc caccacgtca taccagcact
     2941 ccgcgccaat cagtttcccg gcagtcacca gtgaatccag ttttgccctg atggcactga
     3001 taacatcctt cacattcgca ggcgtcagtg gactgtcgat ggtttcaaac tgcgcttccg
     3061 caattgaatc agccagcacc tgtgcggttc gggtatacac ctcaaagatg taggcgttcg
     3121 tttccggtgt gcggttaccc cagaagcgga ccccgttgcg acgaataatg gtcgtgattt
     3181 ctttgttgtt gaggctgttg gcatcgctgt cttcggcctg caacgaccag aacacatgcc
     3241 tcgacatccc cagcacattt ttaaccggaa cgttggacag cgatttgttc catccctgtt
     3301 catggtcaat gtacgcacga aggccgcacg cataggcagg cgcggggaac gtttcgtttt
     3361 tgccattttt cgggttgtag gcgatgaagt ccggccataa gagcatcacc tcacgttcgt
     3421 tgaatttctg gcggtaggta attgcctcgg ccatcgtgtt acagccatga catgaggcat
     3481 acacaaacgc gcgcagttta tctgcaatca cgcacaggga ttttgttacc gcctccgtgt
     3541 ccagctccgg cgcggccaga atacgcggac ggtatccgat gctttcatcc tgctctgcaa
     3601 caagcagcgc atacatcccc atatagctgc cgtcatcctc agaaccaccg ataaccagtt
     3661 gatcctgcgt ttttccgtct tcttctttgt gttcagccac gcgaacgacg atcacctttg
     3721 tgctcacctg gtctgcgatg gccttaagcg cacgataaag cgtccccgtt gtcccgcatt
     3781 ttcccagcac gtcattgacg cgggtcagca gtgtgggctt gttcagcggg aacagcttcg
     3841 cgtccgcatc atccgccgtt gccacgatac cgataacgct ggaatcaaca tcgttaatcg
     3901 ctgttaccag gtcggtattc tccgtaacac gggcaccatg aaaacgagtt tcactcatag
     3961 cttcagcccc ttgtatccgt taaatgattc agcaacaatc atcacccacc acgcgcgtaa
     4021 tctcacccct gcgccgttct cccgccacgg cgacaacaaa aagcagtacc cccctccgca
     4081 cgcacatgcg accatgccgc acagggaggg agcagatgac cgacaccacc atgcaattgc
     4141 tcagtcaggg cacggccccc gtgaaaatgc cggattttga tattctctcg gagggtaaaa
     4201 cgctgtcagg cgtggcggag cgcctgatga gcctgtaact gaccgacaac cggggatttg
     4261 aggcggacca gctcaccatc acgctggatg atgcggatgg tcagttgcag ctaccgccac
     4321 ggggcgcgcg tctgacggtt ctcattggct ggaaagggga accgctgaca gaaaaaggca
     4381 cttacattgt tgatgaaatc gctcacgaag gaccgccgga caggctgact gtttcagcca
     4441 gaagcgcaga ttttcgggat gaatttaacg ttaaacgtga ggtgtcctgg catgatgtga
     4501 ccgttgagcg tgtggtatcc gccatcgctc atcggtacgg tctgaaaccg caaatcagcg
     4561 aaatgctgat ggatatcgaa atcgaccacg ccgaccagac cgaagaaagc gacatgtcct
     4621 ttcttacgcg catggcggaa atgctgggag caatcgccac ggtaaaaagc ggcaatctgt
     4681 tattcatcat gccaggtggt ggcgtgaacg cacaggggca gccgttgccc tcgttcgcca
     4741 ttacacgcag cagcggcgat cgccatcagt tccgcattgc tgaccgcgag gcgtatacgg
     4801 gggtacgcgc ctactggctt gatcttaatt acgggaaaaa gaaaaaagtc agcgtgaaac
     4861 gccgcaaacc gccaaaaccc aaaaaggaga aaagcagcag tcgggaaggc gactatatgg
     4921 aaggtgcgga aggcaacgtg tttgtgttac gcaagactta tcagaacgag caggcagcaa
     4981 gacgcgcagc ggcggcaaag tggcagcagc tacaacgcgg agccgcatca ttctccatca
     5041 cgctggcgcg tggtcgtgca gaactctatc ccgaaatgca tggcacagtg acaggcttta
     5101 aaagcgagat tgattatcag gactggatta ttgcaaaagc cgagcacacc attgataaca
     5161 gcggctttac cacgcagctt gaacttgaag caaaaatccc ggaatggata gcagaaacag
     5221 aataaatctc ttaaaaatat tatttttgag atgtgcttct acactgacag taagaacaaa
     5281 agataatgta tcagcccact agatgtcaga ggttggggca tcaaccagat gctaacaata
     5341 atacggatgt ttttcccaca tgacagagga agtcaagtat aatcttcaca gtccatcccg
     5401 tccaagggaa tatgactcaa tagtaaaagt tccagtttag ttagtagacc cacactttat
     5461 agaagagtaa ttcatgattg gacaattttt aagtgcaaca gagattttag caaagaacta
     5521 tgttcgtaat aaaatggtta aaaatccatt ttattcgaat ttaaaatgga attttattga
     5581 aaaaaacatc ataagattaa catcctcacc ggttaaatca gtactttgca tatcagcttt
     5641 ttcatttgta ctactttatg ttggatattt aaacgaatta tttataaaaa ataatctttt
     5701 acattatttc ccgtttaggc attcattaac cgaatggcaa acaaccattt taagtg
//
LOCUS       DAJTNA010000222         5696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTNA010000222 DAJTNA010000000
VERSION     DAJTNA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.227"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(248..4105)
                     /gene="sigA"
                     /locus_tag="OO161_003711"
     CDS             complement(248..4105)
                     /gene="sigA"
                     /locus_tag="OO161_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5464607.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSEKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFQASTQAIGFSNVSPVISTKEDQEH
                     TTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4466..5469)
                     /locus_tag="OO161_003712"
                     /pseudo
     CDS             complement(4466..5469)
                     /locus_tag="OO161_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5466..5696
                     /locus_tag="OO161_003713"
                     /pseudo
     CDS             5466..5696
                     /locus_tag="OO161_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1420 a         1279 c         1268 g         1729 t
ORIGIN      
        1 cgtttcgtta tactgtccgt atcggaacca tgagaactca gttgcattcc agccatgctc
       61 cagaatatgt gcattttttt gcctgatagt ttcttttttt attcgctcat gtttaaggca
      121 taacaggcag ataaataatg tcagaaaaag aaagctaata atgagagcaa gagcgatata
      181 gtctgtcaca gggtaataca tattctctcc ggtagaagaa gggccgcaaa cgcggcccgg
      241 gctgttatca gaaagagtaa cggaagttgg cgttgatcgc gttatccacg ttgtatttac
      301 caaatgccga tttctcaaac tcaagaccga agcgaagatt atcgcgaatt tcggcgttga
      361 gaccaacatt catgagaata cgaccgtctt tttcaccttt gatacgtttc tcaccggacg
      421 catcacgcag tacggtttca ccgttggcaa acaggtcaaa ctggtagcca aggccggcgc
      481 gggctgtgac tttccagtcc ttaccggaga aggatttacc cacatcaaca ccggtacgcc
      541 caatcagcgg attaaagtcc ttatccttca tggtcacgct cattccgcga tcctgccagt
      601 cgaaccgttt acctgatacg gccccataaa ccagttctgc ctgcggctca atccaggagt
      661 cttctgttac atgatagcgg tagcctgctt cagcacccac atacaaggaa tgagaactgt
      721 agtcttttgt tccgagccca gcaaaggtcg ccgagtactc attatcatgg tgaacatact
      781 tactaatcag gtcgatatag gcaccagagt caaatatagc ggaagcatac agacctgccc
      841 cgacggactt cgtcttgcca ctgaatgcat tactgctggc atgactgtcg gtatacgtca
      901 tagtcagacc agtgaaaagg tcaagtccat ccagctcatg ttttttatcc acaccaatct
      961 gcacatgggt gtagttgtca ctgtatccac cagaagctga acctgctccg ctcatgatgc
     1021 gggcccatgc accggcctcg ccattggtgt cacgcagatc ccccattcgt ttattcaggt
     1081 tgttgacttc tgtcaggaag gctttgtaat taccggacat atacgatttt gctgcactgg
     1141 aagatgctgt attttcagcc accttatatc cggtcagggt ccacgtggta tgttcctgat
     1201 cctctttcgt gctgatcaca ggagatacgt tgctgaaacc tatggcctga gtactggctt
     1261 gaaatatctc tttattgctt ccttttgggg ctgttatcag gtcaacgttt aatcctgagt
     1321 tattatttcc cactttctgt atatcgaccg acagactgtt attgcttccg gttaactgat
     1381 ttttaacaat gagtttatcc gattgattta cagactggct cagatcagcc cgcataatca
     1441 cagcagtatt agtggcatcc agtgtgttta ctgtcacagt atggaaaccc gaattctcta
     1501 tgttactaaa caacaaaaga ctgtcgcggg tttttaatga ttgcagggct gactgtccgt
     1561 ttactctcca taaggtgtta tacatgctgg ctgaggcagc aggagcatca atacctccgg
     1621 tataagaagt atcaaagcca cccagcaaac ctgtagccag cggtgtgtgt gtggttttgt
     1681 ccaggttatc ttctttatac tggtcaacac caaacagaat agatgctttt ttttctgatt
     1741 ttatattacc attgacccag gcataatttt gagcctttaa cgtggcacca tcttctgtca
     1801 tattatatcc atctgacata tagattgccg ggctgtattt attattttct gtaggggtgc
     1861 ctgttaatgt cagggttccg gccagttcaa tagctttatc tacatacatt tttccctggg
     1921 cggtaacagt agctcctttg ttgataatca ggtctgtatt aattaaggat aatgcaccag
     1981 tcatagtaac agttgattta tcaccaacag ttattttgct gttttgagca ctcagactgc
     2041 cgttgaaact actttcgcct gctgaatccc cggagcgaag ctgttgtctg aatccaaaac
     2101 cttcgccagt aatgtttctt cctgagaaca tgtcaatgta tgcagtttta tttcccagaa
     2161 caatttgaga gtttttagcc tctatgtgtc cttttacatt agcatcccgg cctatactca
     2221 gggttgcgtt ttctaaattt aatttccgga aattaaattt acgactttcc cagtcgggct
     2281 gggaaaaaga ggctatctga ttgttggact tatattccgt attgtaacgt ttatttacag
     2341 tgctttcctg gtcctttatg gcagcagaat agtctttttg acataaaaaa ggaattggac
     2401 agttgttatt gccttcttta aatatggcat gttctgtcgc atgtccttgt aatgttaact
     2461 gtgcattccg gacagagata tcgtttttga tatcgactga gccatcaaag atcagattgg
     2521 cattattgtt atgttgctgg gtagtattga taatatgatt tatgcttata ttacctgata
     2581 cattcccatg gtacatatat gcatcagtat tagtaagtga taatgttgat tgcttcacgt
     2641 ttgagttcgt tacggttgtt cccgcatctg ttgctgcgat tttctgaaat gactggtcat
     2701 agccgtttag atccagtgtt ccgccatttc tggtaaaata gatttcgcca ttaccgcttt
     2761 cgcttaaagc gtctttggca tttattttta ccgtaccttt acctccggcc atgtaaattt
     2821 tattgaaggc tttttcagca ctaaggatga cagtcccatt acctatttta aggttatttc
     2881 cctgtgctat ttttacatcc agagtaccac tacctatttt atgcagatta tctccggcaa
     2941 cgcccttaac attccattca acggtggtat tattatcaat atctatcccg gcacctttat
     3001 aagacttatc ttttccgtac actttatatg tctggttctt atcgaatata agcccgccaa
     3061 tgccaagatc ggtatcctgc ttgagcgtta attcccctcc tcctgagaca gacaggtcct
     3121 tatcttttat aatcgatgat aactcttgtt ttttaccatc cagctgcata gtgtttgtga
     3181 aattcttttt taaattgttt accagaatat tattaaataa cgagtagttt gtccaggcta
     3241 tattggtact actgataaaa ccaactgtac tggtaacacc atggaaaacc catttttgtt
     3301 gttctttatc atatagataa gaaccagaac cactatcgcc aaaaattggt gagtttttaa
     3361 aaggggttct gttggtataa atgtaactct tgtttttcca gtccagatta aaaagacttg
     3421 cgcttaacaa gtcgggatta tagttttgtc caaacagata ttgtttgccg tttaatatcg
     3481 ttgaggttgt tccggcacct gctctgaagc caattatttg ttccttaccg ttgtagttta
     3541 cgccatatct tttcatcgca tcttcgggag aaagtgagaa gtcggcactc tccgtaaccc
     3601 ctgttgtttc cacgacgaat ttattcagac gatgaaccga aaagtcgcca gatgatactc
     3661 tgttacttgc tttatatgct gtctgatccc atgactgtgt tgttattgca tgatgctgtg
     3721 taccgttatg agtcgcagta acactgtacg cacctccaat tgatgttgtt gcacctttgt
     3781 tggaaacagc agaaaaatca ggtacaggta gttctggaaa atgaaatatc tttccatctt
     3841 tcatcattaa ttgaacattt gttgcacccg gtctgaactc tcctctgttt tgagcaaggt
     3901 caagataatc gcgggtccat atattattta tatccagcat tgctgcaaat gctgtttcgc
     3961 cataacttcc atatattgag gataaaataa taccgaggat aactttcttt cttgatgtac
     4021 cgacactaac tttccgggtc agttcagaaa cagcaactaa tccacctgta atatgactat
     4081 atttcagtga ataaatttta ttcatacgtg aactccgata tttctgagtt gtggttatgg
     4141 gatagcattt aattacaacg gggaactatc agtcatggta cgtaactgat aagggtataa
     4201 tgataatcac tgtcagttat atttgtatga cagatgtaac aaaatgaaaa aaaagataag
     4261 cacaagtgat tgattggtca gatattgatc aattggtgtt tgtgttttga actttgcgat
     4321 caaaatttga tctttgttgt ttccggtcaa tcctttacag gttttctgta ttatccgggg
     4381 gagttaagtc tgtcgatgtc cgacagactt ttgatgatgg aaacaacatg cagcgataat
     4441 gtgcatgctg ttttgcaggt ggaactcaga aggtgaactg gaactgtgcg accgcaccgt
     4501 aagtgttgtc cgtgccgatc aggcctgcaa catcaaggtg aaccacatca aacggtgaga
     4561 gccccacccc ggccgtgaat gcactgccgt cgttaccggc cagattctga cggtaaccgg
     4621 cgcgcagctg tgcccatttc caggcattaa attctgcgcc aatggcggca aactgacggt
     4681 tgctgtcgga ggtgaaaccg ctggccgggt cagatccaca tcaaatgcgg tggtgaacat
     4741 cgcgttgtgc caggagacac cggccgtcac ctgcgggcgg atcctgaagg tttccgtgat
     4801 accgttcact tcttttgtct caatactacg ggggatcagg ttctgtgcga ccagtcccag
     4861 cgtccagttg tcatctaggt ccgtactggc accgatgtcg gcgttgatgc catttttcgt
     4921 gttgtggtag cggtcgcctt tgaaggcgct gctgtcataa tttctgacca gcacgttgta
     4981 gttgaacagg tcgacgcgct ggaatttggg ggggaagccc agagaccatt tctgcccggc
     5041 cgtttccagt tccgtagcca gagcaatacc cacatcagtg atcagtgccg cgcgggcaaa
     5101 ggcctgtgat gtcaggcggc ttttgtccac ctcctgcccg gtgctgtttg ccacttcttc
     5161 caggtaagtg aggtcagcat cagagacttt accgtcgaca ctgacggtgc cgtgtgattt
     5221 aaccatcagt gcggcagaga ggcgatcacc gggaagggcc gccacagcag aaacgcccag
     5281 ctgtgcggcg gcatgtgtgt tacggaagtc ccggaggtgt tctttcaggc gtgcggcagc
     5341 ctcaggaaca ccgtggttac tttcgatggc gcggtcgaag gccttccact catcactgat
     5401 ccggtcggcg ttgtcggtga tattatccgg attggagagc tgcgcgccga cggacggaag
     5461 caggatgctg ggctcatgcg cgacgcaaaa tccacgatgt atatatcagt actaaaagcg
     5521 cgacggcgga agaagctctg aaactaatcg gtgaactgta cgccatagag cacgatatac
     5581 gcgggttacc ggtgtctgaa cgcctggcgg tcaggcaaat gcagagtaga ccgctcctga
     5641 cttccctgta taagctgatg caggagaaag aacacacgtt atcgaagaaa tatcgt
//
LOCUS       DAJTNA010000223         5695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTNA010000223 DAJTNA010000000
VERSION     DAJTNA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.228"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO161_003714"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO161_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5464608.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO161_003715"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO161_003715"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5464609.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO161_003716"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO161_003716"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5464610.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO161_003717"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO161_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5464611.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1429 a         1354 c         1538 g         1374 t
ORIGIN      
        1 ctcaagggct gtcgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagtggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcaccac gttctgtata gtactcagca acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggttgcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgtg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 atgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggt ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tcccgctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tggtggtaca tcctggcgcg ggtaacctgg atggcacggt actgaatgcg
     1261 ttgcttcatt attatccgcc cattgccgat gtaccgcgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaaaggcg tgaaattact cgtgagtatg aagcggtggc aattggtcat
     1441 atgaccgctg gcggtacagt agatgaacca atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atcccatggg caaaccggcg gtcactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaaccggac gtacgcacca gatccgcgtg
     1621 catatggcgc atattaccca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcgtttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ctatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cagaagatat ggtggagttg attgaggtta tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tctacccgta tcggcggcgt gagcttgcca ccttacgact
     1981 ctctcaacct tggcgctcat tgtggtgata acccggagca cgtagaggag aatcgcaagc
     2041 aactttttgc tgcgggaaat ttgccttcaa aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagctcact ggcgaacctt atgcctcaaa acgggcggat gcctcttata
     2161 gcaatacgcc cggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgattcggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgaggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggtgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gacgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg agtttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaacat tctgttgcag gtactggatg atggtcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatttgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtaa
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtagctg tccagtaatg ataaaacgag cccttcgggg
     5341 ctcgtttttg tctataagtt agacggaaaa gactatattt aagatgtttt gcctgaaaaa
     5401 tgagcgaacg ataaagtttt tatatttttc gcttgtcagg ccggaataac tccctataat
     5461 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
     5521 gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt agcgggaaag tgtattatac
     5581 acaccccgcg ccgctgagaa aaagcgaagc ggcactgctc tttaacaatt tatcagacaa
     5641 tctgtgtggg cactcgaaga tacggattct taacgtcgca agacgaaaaa tgaat
//
LOCUS       DAJTNA010000224         5596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTNA010000224 DAJTNA010000000
VERSION     DAJTNA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.229"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(122..1460)
                     /gene="adiC"
                     /locus_tag="OO161_003718"
                     /pseudo
     CDS             complement(122..1460)
                     /gene="adiC"
                     /locus_tag="OO161_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
     gene            complement(1597..2358)
                     /gene="adiY"
                     /locus_tag="OO161_003719"
     CDS             complement(1597..2358)
                     /gene="adiY"
                     /locus_tag="OO161_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5464612.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(2683..4950)
                     /gene="adiA"
                     /locus_tag="OO161_003720"
     CDS             complement(2683..4950)
                     /gene="adiA"
                     /locus_tag="OO161_003720"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5464613.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(5149..>5596)
                     /gene="melR"
                     /locus_tag="OO161_003721"
     CDS             complement(5149..>5596)
                     /gene="melR"
                     /locus_tag="OO161_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5464614.1"
                     /translation="PEIGLMLKRFSLSGWEPILVNKTSRTHKNSVSRHTQFYVSQMLG
                     FIAENYDQALTINDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTD
                     KSILDIALTAGFRSSSRFYSTFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         1439 a         1321 c         1427 g         1409 t
ORIGIN      
        1 gcaggaagga catctgatgg aaatggaagc cacgcaagca ccttaaaatc accatcatac
       61 actaaatcag taagttggca gcattaccgg ttggctttta tgtttgctgg attaagggga
      121 attaatcttt gcttattggt gcatctaagg gatacgggtt tttatgtagc cggttgtaat
      181 tcagcgcata catggcggtg atgaccatca gggtgacaaa tgaccacata acctctttcg
      241 ctccggaccc caccacggcc cagatgcagt agaggaaggc aatggtagta actgccagat
      301 atgccgggcg tgctttacca aagtgaccgt gtccgagcag cagtaacgcc gcacaggtgt
      361 aaagatatgg caccagtgta aagatgaccg agacggaaga aaccagaccg aactctttgg
      421 tcgcgtttgg tgaaatgctg ctgagctgga agatggtcat caaaataccg acgataatca
      481 gccccgccac tggcgtaccc gctttattta cacgggcaaa aatcggtggg aacagtccgt
      541 catcggcagc ggctttcgcc gtttgacccg ccagcaacgt ccagccgccc aagtgaacct
      601 aagcaacccg cagctgcgca gaaggaaaca atggccccgg cggtgtcacc caacgccatc
      661 cgtgcggcat caccgaatgg cgaagcagaa acgcgcagtg cggcattagg gatcatcccc
      721 ataatcgcgg tggtagaaag tacatagcaa acggcggcaa tcaatacccc accaatggtg
      781 gcgataggga cattgcgttt cgggtttttc accacacctg cggcaacgga ggcactttcc
      841 acaccgatga acgaccacag cgtaacgtta agggtacttt gaattgcacc gaaggtgccc
      901 aggccgctga cgttccatgc cgccatatag gtttcaccac ggaaccagaa ccagccaaat
      961 acggcaatcc cgacgatggg gatcagcgcc agcacggtgg caactgcctg cacacgggtg
     1021 atcattttcg gaccgacaat gttcagcagg acgaagatcc acagcaccac gacgcaggtg
     1081 atggttaata ccaatggatc tttcagaatc gggaagaagt aacttaaata tcctacgcca
     1141 atgaccacca tggcgatatt gccgatccag caggccagcc agtagaggac gttggtttga
     1201 taaccgagaa acgggccaaa gcagcggcgg gcgtaagcgt aagaaccacc aggacttggg
     1261 tcgaggaacg acattttggc gtataccatc gagagcccca gtgcaccgat aatcgtcacc
     1321 aaccatccat aaatggcaat cccgccagtg gaggccaggt ttgcaggtaa cagaaaaaca
     1381 cctgacccca taatattccc cgacaccatc agggtgacgg ggattaagcc cactttgtga
     1441 gcatcagcat ccgaagacat aattaaactc ctgcgaaggc gagcttcgtg acaataaatt
     1501 acgtcatatc atacgcctgc attcgatagt tgaattatta tcgaccgggt tatcgctgat
     1561 gccgttatta atatagctct tcgttaaggt taaaaatcag gcgacagtgc gttctctgtg
     1621 ctgactaaca taatgcagcg gcgtcatacc gtagaagtct ttaaagacag aaataaagta
     1681 cgacgtactg ttgtagccgc aggactgtga tacctgagag atatttttac cgtccatcat
     1741 taattcattt acggcataac gcatgcggca ggtggtgatt atttggctat aactggtgtt
     1801 ttcgcttttc agctttttct ttaacagact tgggctaaga cataaacaac tggctaccat
     1861 actaagattc cagtctttgt gaatatcgct ttcaataatt tgataaacgc tgtcacttac
     1921 acaattacgt aacatataca tcattaatga aaggaatttt ttactgtcga gaaaacggga
     1981 tagcaccgtg aataataatg cgcgtgttcg ttccttttct gactcagttt ctgcgggcat
     2041 catgctgtgt tgcgcggcaa cgcgaaagac gtctggcgtc aggcatggca gggtgagtat
     2101 gggcgtagcg ctacgttgcc atactggtat ttgcgagaga tctttattca gaaaccggag
     2161 gtaatctttg atgatgtcgt gactaatatg agcaaccaaa gtgttgttaa gagaggagac
     2221 gtcgataata ttattttcac aatttaataa cgccatatgg ttagctttaa ggctaatagg
     2281 ttctttccca ttcaccctta tccagacatc tttttcagtc aataaaacaa tacaaggttg
     2341 gtcgctgcaa atcctcatac atgtactcct gagtgcgaat aaaagaccag ttctcgctaa
     2401 agcaaagcga taccgggaaa tggttatata attgtatgac gaatataaac atgcataaaa
     2461 ataacgagtt ttactaatat tacaaatttt tatagtaatt tctcatgtac atattttttt
     2521 acagggtaaa taacaggcaa aaaaaagcgc ggggtggggc cgcgcaaaaa gtatgacatt
     2581 tttgtttgca gcaattttta tgaaaacgtt gccatactca aaaggcctaa ggccaggcgg
     2641 cgtaatgtta tttaaacaat tacgccttca gcggaatagt ggttacgctt tcacacacat
     2701 aacgtggtaa ataccgtcaa taatttcagt cccttcagtt tcgtgttcaa atccagggaa
     2761 atggtggtcc caggattgca gcgagcgtaa ataacttact tgcggactgt ttttatcgcc
     2821 gaagttttca ccagacagca gcatcgggat tcctggcgga tatgggataa ctgagtttgc
     2881 cgcgatgcgt cctggcagat tttcaatgga taccagttcg acattgttgt cgacaatcgc
     2941 gttgtacgct tcacgcgggg tgatttccgc caccggcagg ccggaatagg cttcgttcaa
     3001 ccgtgcgcca gggttgtttt ctttcagcca ggcaaacatg gtgtcaccca gatcgtgaat
     3061 ccccatgttc gcgtaagtgt caggatattg ttcaacaagt tccggcatca cctgcgccag
     3121 cggtgtgttg gcgtcatagt ggcgtttgaa ggagcaaagg gtgttaacca gagtccccca
     3181 tttcccacgg gttacgccca tagagaacag gaacataatt tggaagtcag tggtgcgggt
     3241 aggcacaatg ccgtggcgac caagccaggc agtgaccagc gccgccggaa caccggtttc
     3301 ttccagttca ccatcttcac ccattcccgg agcaaggatg ctgactttaa tcgggtcgag
     3361 catactccag ttatccggaa tatctttgaa gccgtgccag ctttcgcccg gatgcattac
     3421 ccagtagtcc tgaacggtgg tcagcagttt ggttggtgcg tcagcaaagt cataggtttt
     3481 gccggtttgt gggtcggtga cgacttcttt gttccacggt ttgaagaacc agctaccgtc
     3541 agcggtgaac tctttataca gccgcgccat cgcctgacgg aaatcaaccg cttcgtcgat
     3601 cacttcctgc gtcagtgaca ggccgctgtt gccgtccatc atcgacaccg ccacgtcgtt
     3661 ggatgcgcag atggcataca gcggggaggt ggtggcatgc atcatgtagg cctggttgaa
     3721 gcgggagaag ttaatcgccc cacgaccttc acgtacatga atataagaag cctgtgaaag
     3781 cgcattcagc agtttgtggg tggagtgggt ggcgaaaacg gtaggaccgt tgtgatcgcc
     3841 aggttcgccg cgcatggcat agtgatcggc atagatcggg ttgaaacgtg catagccgta
     3901 ccaggcttcg tcaaagtgca gacgatcgga ggttttttcc agcagatcct gcgcttcttt
     3961 agcgttataa cacacgccgt cataggtgca gttggtcacc acgcagtaag acggtttttg
     4021 cccggctttg tctttagtca gcgggctttc actgattttc ttctgcaagg tttcaggttg
     4081 catttcctgc ggatagattg gcccgataat gccgtagcgg ttgcggcttg gcaccatgta
     4141 gaccggtttc gcgcctgtca gcatcaagcc ttgttcgatg gatttatggc agttacggtc
     4201 aacgaccacg acatcgttat cggtcatgca agcctgcatg atggtgcggt tagagccgga
     4261 agtaccgacg actaccgacc aggagcgatc ggcaccaaat acgcgtgcgg catatttttc
     4321 gctttcgcca aatgcgccag tatggtcaag caaagaaccg agggaagttc gttcgatgcc
     4381 catatcggtg cggaacagat tttctccata gtagtcatgg tagaaacgtc cggcgggcgt
     4441 tttggtaaaa ccaacgccgc cctggtggcc tggcgctgcc caggaatatt catggatgtc
     4501 gctatatttc atcagcgcgc tgaacagtgg cggcaacagc tgctggcggt agcgggtcat
     4561 cgcggcaacg gcgcgtccgg cgataaagtc ggtggtatct tccagaatcc aggcgaattc
     4621 atcgacaagc tccagcaggt cgcgatccat tgcggcgagg gctttttccc gatcgcccaa
     4681 caggaagacc ggcacgtttt gttggcgctc atgaagctta ccgatcaatt gtctgacgtt
     4741 ttgatgttcg tccggatgtt ccatttgata gctgaacatc aggcagtcaa tggcttcgtt
     4801 tgaagagaga atggcaaaac catcatcaaa ggaggtggat ttaatcacgg taacattttg
     4861 ctggcttaaa gcatctgcca gacgctcaac ggcgttaccg acccaggtgt cttgatggag
     4921 aaactcgctt tcaacaatta atactttcat cattgcttac ccggttatga aggaatcttc
     4981 gttgcgggca agtatcttcc ggccttttcg ggctgtaaag cgcgtgaaaa acaaacattt
     5041 ttgaaatatt tatttttata aacaatgcgt tatgtgatta ttttaaatta ttttcactgc
     5101 ttgttatcga cggctaaact atttttttgg ctgatactga tatcgtcttt agccgggaaa
     5161 cgtctggcgg cgctgttggc taagtttgcg gtattgttgc ggcgacatgc cgacatattt
     5221 gccgaacgtg ctgtaaaaac gactacttga acgaaagcct gccgtcaggg caatatcgag
     5281 aatactttta tcggtatcgc tcagtaacgc gcgaacgtgg ttgatgcgca tcgcggtaat
     5341 gtactgtttc atcgtcaatt gcatgacccg ctggaatatc cccattgcat agttggcgtt
     5401 aagtttgacg tgctcagcca catcgttgat ggtcagcgcc tgatcatagt tttcggcaat
     5461 aaagcccagc atctggctaa cataaaattg cgtatggcgc gagacgctgt ttttgtgtgt
     5521 gcgcgaggtt ttattgacca gaatcggttc ccagccagag aggctaaatc gcttgagcat
     5581 caggccaatt tcaggt
//
LOCUS       DAJTNA010000225         5490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTNA010000225 DAJTNA010000000
VERSION     DAJTNA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.230"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..410
                     /locus_tag="OO161_003722"
     CDS             <1..410
                     /locus_tag="OO161_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5464615.1"
                     /translation="SWRKERRTSYSREFKVRLAKQALQPGAVVARIAREHDINNNLLF
                     KWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDERPEGAPGNVPR
                     CELHLKSGVVKLFDPLTPELLRALIREMKGGTR"
     gene            407..574
                     /gene="tnpB"
                     /locus_tag="OO161_003723"
                     /pseudo
     CDS             407..574
                     /gene="tnpB"
                     /locus_tag="OO161_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099485.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            720..1739
                     /locus_tag="OO161_003724"
     CDS             720..1739
                     /locus_tag="OO161_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR5464616.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
     gene            2014..2196
                     /gene="tnpB"
                     /locus_tag="OO161_003725"
     CDS             2014..2196
                     /gene="tnpB"
                     /locus_tag="OO161_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5464617.1"
                     /translation="MLWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEG
                     IAWQHPKRTERPGIRI"
     gene            2216..3817
                     /locus_tag="OO161_003726"
     CDS             2216..3817
                     /locus_tag="OO161_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5464618.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIP
                     LYRQSEIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVL
                     APGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADA
                     YAGYNVLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGS
                     PAEERLAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDG
                     WVEIDNNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLRE
                     VIEKLNDWPSNQVHELLPWNFSSVK"
     gene            3817..4314
                     /locus_tag="OO161_003727"
                     /pseudo
     CDS             3817..4314
                     /locus_tag="OO161_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558264.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4473..5111
                     /gene="ospI"
                     /locus_tag="OO161_003728"
     CDS             4473..5111
                     /gene="ospI"
                     /locus_tag="OO161_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5464619.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            5226..5490
                     /locus_tag="OO161_003729"
                     /pseudo
     CDS             5226..5490
                     /locus_tag="OO161_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1531 a         1221 c         1491 g         1247 t
ORIGIN      
        1 gctcatggcg aaaagaacgg cgaaccagct atagccgtga atttaaggtc cgtctggcta
       61 agcaggcgtt acagcctggg gctgttgttg cccggatcgc cagagaacac gatatcaata
      121 ataacctgct gtttaaatgg aaaagccagt acgaggacgg cttactgagc gatgatgaca
      181 tacaggaatg catgcctgtc ccggtggcac tgactgatac gccggagccg accagaccag
      241 ttacaaatcc cttctggcgt aacaagcatg atgagcgccc tgagggggct cccggaaacg
      301 tcccacggtg cgagctgcat cttaaatcag gtgtggtaaa actgtttgac cctctcactc
      361 cggaactgtt acgggcgcta atccgcgaaa tgaaaggagg tacccgatga taacgctgcc
      421 aaccggtacc agaatctgga tcatcgctgg catcacagat atgcgttgtg gcttcaatgg
      481 cctggcttcg aaggtgcaga acacgctgaa agatgacccg ttctccgggc atatcttcgt
      541 cttccggggc cgcagtggca aaatggtgaa aatgtaatga acgcatccca cacaattaaa
      601 cacggagcag tatgatggtt aaaagggtga aactggcagg tgtggagcct cgcagatttc
      661 cggcatcata gtgcccaata cgggatgcat aaagctccct cagactggag actccacaaa
      721 tgaaatatac accggttggc gttgatatcg caaaacatgt cattcagatt cacttcatca
      781 atgagcacac aggtgaagtg gttgataaac agttgcgtag acaggatttt ctgacgttct
      841 tcggcaaccg tgagccatgc ctgattggta tggaggcctg tggaggttct cagcactggg
      901 cacgggaact gacaaaactt ggtcataaag tccggttgtt gcaggcccgc ttcgttaagg
      961 cattcgtcat gggcaataag aatgatgtga tggatgcccg ggctatctgg atggcggttc
     1021 agcagccggg taaagaaatc gccgtaaaaa cagaagaaca gcagtcggta ctggttctgc
     1081 accgtacccg catgcaactg gtgaagttcc ggaccgcaca aattaatgcc ctgcacggga
     1141 cgttactgga gtttggtgaa accatccaca aaggccgggc agcgatggag cgggagttcc
     1201 ccgaagcact ggaacggatg aaagagagac tgccaccgta tctcattatg gttctggaaa
     1261 accagtacaa ccgactgaat gagctggact cactgataga ggatattgaa aaacagctta
     1321 ccagcgtggc gaggcagaat gaaacctgta agcggttgct ggatattcct ggcgttggac
     1381 cacttattgc gacggcagcg gtggccacca tgggggaagc atcagcgttt aaatcggggc
     1441 gagagttcgc cgcatatgtt ggtctggttc caaaacaaac tggctccgga gggaaagtac
     1501 gtctgctggg gataagcaaa cgtggtgaca cttatctcag gacattattt atccacggtg
     1561 caagagcggt ggcattagta gctaaagagc ctggcccgtg gataaccgaa ctgaaaaaac
     1621 gtcgtccagc cagtgtggca atcgtcgcca tggcaaacaa gctggcacga acagtatggg
     1681 cgataaccgc ccatgaccgt aagtatgaca ggaaccacgt cagtatcaga ccatattaat
     1741 cgctgatacc attaaacaat gaactcttaa caaaagggtg aatgctgaaa ggttgctatg
     1801 gcggccagag tgatgacaaa gacaggtaag accgtgactc actaaacctg aacagtattt
     1861 tgggcttgaa gtccgccgtg aaaataaggg gtgagtcggc gaattacata ggggctcgca
     1921 gcgttacggc tgcaataaag ccggatataa agctgcaacc tacccgtcat gtcaaaacaa
     1981 tggatgcctt gcaaacggga tgcgttcata taaatactgt gggccgatcg tgacgggtta
     2041 tgcctgttcg ccaaacgcct ggaacggggc cgcttcgtct ggccggtgac ccgggaaggg
     2101 aaagtgcacc tgacgccagc tcagttatcc atgctactgg aggggatcgc gtggcaacat
     2161 cccaaacgga cagaacggcc tggcatccgg atataacccg tgataaaaca agggaatgaa
     2221 caatgaactc cccgatgata ttgagctgct taaagccatg ttgcgtaagc aacagagtcg
     2281 gcttcgacag tatgcctgtc aggtcgcggg ctatgagcag gaaattgaac ggctgaaagc
     2341 gcaactcgac aggttgcgtc gtatgttgtt cggccagagt tcagagaaaa agcgtcataa
     2401 gcttgaaaat cagatccgac aggcagaaaa acgactgtcg gaactggaaa accgactgaa
     2461 cacagccaga aatcttctgg aagatgcatc gtcagtcaca gattcacctg acaccagtcc
     2521 cccgtcagaa aacccgatcg ccagtaagcc tgaatccccg ggacgaaaat cttcacgaaa
     2581 accgctgccg gcagaacttc cccgggagac acatcgcctt ctgcctgctg aaaccagttg
     2641 cccggcctgt ggaggtgttc tgaaagaaat gggggaaaca atctcagagc aactggatat
     2701 cattaatacc gcctttaaag ttatcgaaac catacgtccc aaactggcct gtagccggtg
     2761 tgatgtcatc gttcaggcac cacttccccc taaaccgatc gaacgcggtt atgccagtgc
     2821 agggttactt gcacggatcc tggtcagcaa atatatggaa catatccctt tatatcgcca
     2881 gtcagaaata tacgcgcgac agggcgtgga gctgagccgt aataccatgg tgcgctgggt
     2941 atcagaaatg gcagacaaac tccgtcctct gtatatagcg ctgaatgact atgttctgga
     3001 ggcaggaaag gtgcacgcag atgacactcc ggtgaaagta ctggccccgg ggaacggaaa
     3061 gacgaaaacg ggtcgtctgt gggtatacgt cagggatgat cgtaatgcgg gttcatccct
     3121 gccggcagcc gtctggttcg cgtattcggc agatcgcaaa ggagaacatc cgcagctcca
     3181 cctggcaaag tatcagggcg tactgcaggc tgatgcctat gcaggttata acgtactgta
     3241 cgaaacgggc cgggtgaagg aagccgggtg cctggcccac gcccgccgaa aaatccatga
     3301 cgaggatgtg cgccgtccga cagaaatgac tcaggaagcg ctcagacgga tagcagagtt
     3361 atacgacata gaagcggaga tacgtggcag tccggcagag gaacggcttg cagtcagaaa
     3421 agccagaagc gtccagttga tgcagtcatt gtacgactgg atacagttgc agaggaaaac
     3481 gctgtcgaaa catgcggaga tggcgaaggc gttcgactat atcctgaatc actggaatgc
     3541 gctgaacgag ttctgtcgtg acggctgggt ggaaatagac aacaacatcg gtgaaaacgc
     3601 gttacgatcg gtggcggttg gaagaaaaaa ttatctcttt ttcggctcag acaagggagg
     3661 agaaagtgcg gcgatcatct acagtctgct ggtcacctgc aaacagaacg aagtggagcc
     3721 ggaggactgg ttgcgcgaag tgatcgagaa gctcaatgac tggccgtcga accaagtgca
     3781 tgaactgctg ccctggaact tctcgtctgt aaaataatcc ttacgctacg tacttctcgg
     3841 ggcgcttaca ccgttactgt tcattgtccg cgctgtcatt ccgatgaagt ttatcgacat
     3901 ggccgtagtt gttcccggca tgagcgtttc cgttgtcgct catgtaaacg tgttttccag
     3961 ctcacttact cttatgaagc cagaaaaccc ggcgttaagg agcagatcgt tgaaatggca
     4021 cataacggcg cagggggccg tgataccgcc aggacactga aaatcggcat caataccatc
     4081 atacggacat taaaaagctc ccgccccggc ggataacatc tgcgccagtc gctcacgctg
     4141 atgttgccct tatctgtgaa cttgatgagc agtggcgctt cgtcggcagc aaggctgcaa
     4201 caacactggc tctggtatgc gtacaacact aaaactggcg gtgtgttggc ttacactttc
     4261 gggccacgga atgatgagac ctatcgggaa ctgttagccc tgtttactcc gttctgtata
     4321 tattaggccg ttggtggtaa gtggatcact ttttagactc gaatagctgc agttcacaaa
     4381 aacaggaatc cggctccgtt ttttgttgtc atatcagagg gatatttgtt atgaagaaaa
     4441 tatagtgagt atatatactg tcaggataca atatgattaa tggggtgtcg ttacagggaa
     4501 ctgctggata tgaggcacat acagaggagg gaaatgtaaa tgttaaaaaa ttgctggaat
     4561 ctttaaactc taaaagtctt ggtgatatgg ataaagattc tgaattggca gcgactttac
     4621 agaaaatgat aaatccgtct ggtggggatg gtaactgttc ggggtgtgcg cttcatgctt
     4681 gtatggctat gttagggtat ggagtaagag aggctcccgt acctaatgaa atatcagagt
     4741 atatgaccgg attttttcat aggcatttag agcaaattga tagtgaaggc atagtgtcac
     4801 atcctaatga aacttatagt aaatttcgtg agcgtattgc agaaaatatt ttacaaaaca
     4861 cttcaaaagg aagtgtagta atgatttcta ttgaacaggc cactcattgg atagctggat
     4921 ttaacgatgg tgaaaaaatt atgtttttgg atgttcaaac aggaaaggga tttaatcttt
     4981 atgatcctgt agaaaaatcg ccagatgcat ttgtagatga aaatagttcg gttcaggtaa
     5041 ttcatgtatc tgatcaggaa tttgatcact atgctaacag tagtagttgg aagagtaaga
     5101 ggctttgctg atgctgagag cctgtacata gatttgagcc ggccccttga aacgccagac
     5161 agtggatgta tcttaaaata agagataggc ttggatatat gtctaacact agttctaatt
     5221 ttgagatgac tggaactttg ctggggctgg agcttcgtaa acgtaaaact cctcaggaaa
     5281 aaattgccat tattcagcag acaatggagc ctggcatgac ggtgtcccat gtggcgcgtt
     5341 tgcatggtat tcagcccagc ctgttgctta agtggaaaaa ataatatctg gaaggaagtc
     5401 tcactgttgt tgctgccgga gaggatgtcg ttcctgcctc agaactggct gccgctataa
     5461 aacaaataaa agagttacag cgcttgctgg
//
LOCUS       DAJTNA010000226         5481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTNA010000226 DAJTNA010000000
VERSION     DAJTNA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.231"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(22..594)
                     /locus_tag="OO161_003730"
     CDS             complement(22..594)
                     /locus_tag="OO161_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5464620.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OO161_003731"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OO161_003731"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5464621.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1720..3006)
                     /gene="amtB"
                     /locus_tag="OO161_003732"
     CDS             complement(1720..3006)
                     /gene="amtB"
                     /locus_tag="OO161_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5464622.1"
                     /translation="MKIAMIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(3036..3374)
                     /gene="glnK"
                     /locus_tag="OO161_003733"
     CDS             complement(3036..3374)
                     /gene="glnK"
                     /locus_tag="OO161_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5464623.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3555..5336)
                     /locus_tag="OO161_003734"
     CDS             complement(3555..5336)
                     /locus_tag="OO161_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5464624.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5329..5475)
                     /locus_tag="OO161_003735"
     CDS             complement(5329..5475)
                     /locus_tag="OO161_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5464625.1"
                     /translation="MQHGHIAQRGNHDELAQQSGWYRDMYRYQQLEAALDDAPEIREE
                     AVDA"
BASE COUNT         1331 a         1595 c         1362 g         1193 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagct ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgca ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgcaccg gcaccaatgt caggtcggct ttagttccgc ccttgttgat
      181 caccggctga acggtatcgg taataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcgg tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgccggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aataagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagattattg cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactggc
      841 attgttaaat ctggaaaaaa ttgaggcagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgat ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcacggca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgtggctt tgtgcgcggt gagttttata cccaggacgg
     1621 cgtactggtt gcctcgaccg ttcaggaagg ggtgatgcgt aatcacaatt aaaaaatagc
     1681 cggaggtgaa aaccgtccgg ctgttttttg cagtgcttgt tacgcgttat aggcattctc
     1741 gccgtgactg ttgacatcca gcccttctag ctcctgctct tccggtacac gcagaccaac
     1801 cgtcagatcc gccaatttgt agccaataaa tgccaccaca ccggaccaga cgatcgtaat
     1861 ggcgatgctt tccagctgta ccagcaactg atgacccatc gtcacacctt cagcgaagcc
     1921 cacgccgccc agcgagctgg cggcaaaaat cccggtcatg atacagccga caatgccaca
     1981 aacgccgtgc acaccgaaga catcgcaggg atcatccacc cgcagcaagc gtttgagcat
     2041 ggtaacgccc cacaagcccg ccagaccagc taccacgccg ataatcaacg cgccgccaac
     2101 cccaatgtag ccgcacgctg gcgtcacgcc gaccagaccg gcaatcgcgc cagaacacgc
     2161 ccccagcagt gaaggcttac cacgcagcgc ccattcaccg aagatccagc caagaattgc
     2221 cgccgccgtt gcgaccacag tattcacaaa tgccagtgcc gcgatttcat tcgccgtgcc
     2281 cgctgacccg gcgttaaagc caaaccaacc gatatagaga atggcagtcc cggtgaagac
     2341 catcggcagg ttgtgcggtt taaacgcctc tttaccgaag cccacgcgtt tgcctatcag
     2401 atacgcgccc accagaccgg cgattgcggc gttaatgtgc accactgtgc cacccgcgaa
     2461 atccagtgca ccgtgagaag ccagcaaacc gccgccccac accatatgcg caatcggaat
     2521 gtaagagagc gtcagccata ccaccacgaa aatcaacaca gctgagaagc ggattcgttc
     2581 cgccagcgcc ccaactatca agcctacggt aatgcaggca aacgatccct gaaacgctac
     2641 gtggatatac tgataaatgc tgcccatcac cgccgtcagt tcgatgtttt tcagcatcaa
     2701 ccagttaacg ttgccgaaga agttgttacc ctcaccaaac gccagcgagt aaccgtaaac
     2761 cacccagaga atacagacca gtgcaaacgt caccgtcacc tgcatcagca tcgacagcac
     2821 gtttttgccg cgaatcaacc caccgtaaaa cagggcaatc cccggaatag tcataaacag
     2881 caccagcgca gtacaaatca tcataaacgc attgtcggct ttatcggcca ccgcaggtgc
     2941 agccattacc agtcccggca gcatcgccag tgaagcaagc ccagttttta tcatcgctat
     3001 cttcattttt cgttcctgtt gctgtgtgcc agagattaca gcgccgcttc gtcggcttcg
     3061 ccggtacgaa tacgaatgac gcgttgcaat tcagcgacga agattttgcc gtcgccaatt
     3121 tttccggtgt aagccgcctt actgacgata tcgatcactt catcgagttg gtcatcagca
     3181 attgccacat caatttttac ttttggcagg aagttgacgc tgtattccgc cccccggtac
     3241 agctcggcat gccctttctg acgcccgaaa cctttcactt cggtgacggt caggccctga
     3301 ataccaatgg aagataacgc ttcacgaacg tcttccagct tgaatggttt gattatcacg
     3361 gtcaccagct tcatagatcc cctccagtca gaattcggta atggttctgc tacacgaaga
     3421 aggtattgca agcggtgtgc cagaaatgaa aaacgagaaa gagagcagga agttaagaag
     3481 attgaaaatg aaaacgcact atgacagtgc acagtgcgtt acatttttgc accaacgatg
     3541 aaatattgcg ctattcaggc gctcaatgat tcttcttcac gcacgctggc tgccagctct
     3601 tcgcccgcaa gttgcagttg atacatctgc cagtagcgtc cctgggccgc cagcagttgc
     3661 tggtgagtgc cctgctccac ggcttgccca cgatgaagca ccagaatggt gtcggcatca
     3721 acaatggtcg ataagcggtg agcaatcacc accagcgtgg tatgttcacg cacagccgcc
     3781 agagcatgtt gaatcgcctg ttcagtaccg gagtcaatgc tggcggttgc ctcatcaagg
     3841 atcaggattt gcggcgtctc caccaacacg cgtgccagtg ccagcagttg cttttgtccg
     3901 acggagagat tattcccctg ctcgcccagc ggcgtataaa taccatcact catgctgcgc
     3961 gccagctccg ccagttgcac ggtttccagc gcctgccaga cgcgttcttc ggagatatca
     4021 cgccccagcg tcacgttggc gaggaaggta tccgccagca ccaccggatc ttgctgcacc
     4081 attgccacgc cctggcgcag cgcgctgtga cttagcgaac ttaatggacg accatcaagg
     4141 cgaatctcac cttccgttag cgggtaatag cccatcaata aactggcgag ggtgcttttg
     4201 ccactgccgg tatgcccgac cagtgccaca aagttacgcg aaggcacaga gagattaatg
     4261 ttctttagca ccagattgtc atcgcgataa gcaaatgaca cgttatcgac ttcgatggtg
     4321 ccactctgta acgggcgatc atcattgcca tattgctggc gcggtccgtc catcagttca
     4381 aacacgcgct caccagcaac aacagcctgt tgcagcatcg cctgttgcgt ggtcagttcg
     4441 attaagggtt cgttaagtcg cccaagatag ctgataaacg catacagcac gcctacttca
     4501 atggtgccgc tagcggagaa gccaaacagc atcaacaagc cacaaagaat gagcgatgaa
     4561 aacagactca gcagcggacg cagcagaaaa ccgtcgaggc gcagggtttg catcctggcc
     4621 atatagtgtg aacgactggc ctcccccata cgttcgccaa atcgcgcctg ctgacggaac
     4681 tgctggataa cgctcatgcc attgatgatt tcgttaaagc cgtcgttgat atccgccaga
     4741 taggcgcgca cacgacggac aatcggcgtg ctgtaacgct ggtatatcac cattaccacc
     4801 agcaccaccg ggaaaatcat tatcgccacc agcgccattc gccagtcgag gctgaacatc
     4861 gccaccagca tcgcgcccac cagtgcggcg ctacgcagga cagttgccac tacggtaacg
     4921 tagagatcgc ggatcacttc agtgtcatta gtgacgcggg aaatcacctg cccgacgggt
     4981 tgggtatcaa actcgcttaa tggctggcgc aatgcggcat ccatcacgtc ggtacgcaac
     5041 tgttgcacta cgcctactgc cgcccgatta aacagcagcg actgcgcgta atgtagcccg
     5101 gcggcaaaca gttgtagccc aacatacgcc gcagccagcc ctgcaaccat tttcaacggc
     5161 aggttatttt tcgctaccat attgtcgata aaatagctga taagcagcgg cccactgact
     5221 tctgccgctg ccgcaaccca catcatcagg accgcaatcc ccagcggttt acgccacggc
     5281 gaaccgtacg ctaacagacg cttgagagtc ggccacagtt ggctaaaact acgcatcgac
     5341 ggcctcctcg cgaatttccg gagcgtcgtc gagcgccgcc tccagttgtt gatagcgata
     5401 catatcgcga taccagccgc tttgttgcgc cagctcatca tgattgccac gctgggcgat
     5461 atgtccgtgc tgcatcacaa t
//
LOCUS       DAJTNA010000227         5435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTNA010000227 DAJTNA010000000
VERSION     DAJTNA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.232"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..234
                     /locus_tag="OO161_003736"
     CDS             <1..234
                     /locus_tag="OO161_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5464626.1"
                     /translation="MFNTRLYRVNFDVASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(388..2037)
                     /locus_tag="OO161_003737"
     CDS             complement(388..2037)
                     /locus_tag="OO161_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5464627.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2189..3538)
                     /gene="ghxP"
                     /locus_tag="OO161_003738"
                     /gene_synonym="yjcD"
     CDS             complement(2189..3538)
                     /gene="ghxP"
                     /locus_tag="OO161_003738"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5464628.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILHNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKKPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4083..4547)
                     /gene="soxR"
                     /locus_tag="OO161_003739"
     CDS             complement(4083..4547)
                     /gene="soxR"
                     /locus_tag="OO161_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5464629.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4633..4956
                     /gene="soxS"
                     /locus_tag="OO161_003740"
     CDS             4633..4956
                     /gene="soxS"
                     /locus_tag="OO161_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5464630.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(4959..>5435)
                     /gene="pdeC"
                     /locus_tag="OO161_003741"
     CDS             complement(4959..>5435)
                     /gene="pdeC"
                     /locus_tag="OO161_003741"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5464631.1"
                     /translation="RLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMTPIILAFRQ
                     AGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHIIELAHSL
                     GLKTIAEGVETEEQVNWLRKRGVRYCQGCFFAKAMPPQVFMQWMEQLPARELTRGQ"
BASE COUNT         1467 a         1517 c         1294 g         1157 t
ORIGIN      
        1 atgtttaata cccgtctcta tcgggttaat tttgatgtgg ctagcgtcca gggaatgggc
       61 atttccatac tccgtggaga ggaaaatatc cccattgata gtgataccct ggtaacacta
      121 cagaaattct ttgaagaaga ttgtacctct catactggca tgtcacaaac tgaggataat
      181 attaatgcag tcgctatgaa gattactgca gatattatgc aacacgcaga ttgaggttaa
      241 gtcgaagaac agccaatgaa ttgactttac aagagagata acttatttgt tttagaatca
      301 tgaatgttag cccgagctaa cctgttaaag gtgcaaaaga ttgcaccttt ttcagattac
      361 ctcgggcctg gttacgtcgc tttcggctta ctgattttcc tcaattagca acgcttcaag
      421 caaatcgaga tcgtgcagta atttttgcag cgtttcgttg ctgatctccc gcgtggcgcg
      481 caggtggtac agttcagcac gttcagaacg caatgccgcc agacggaagc gacgctcaag
      541 gttctcttcc tgcacggaac tttcaacgtc gttacgtcca tcggcgcgac gacgcaggtt
      601 accaatgacg cgagaactga cctccgtaag cagctggtta tcgatattct cttcggtatc
      661 cgccgccaga cgctcctcca ttttctggat cgccacaatc gccacttctg ccgttgccgc
      721 tcgcgcaata cgttcctctt tcaattgttg cgaatgatcc gcgacttcaa tgtgttgtag
      781 cagaataggc aacatcacca cgccgacaaa cagcgagaag agaatgacgc cagccgccag
      841 gaacaccagc tcatagcgcg ccgggaagac gttaccatcc ggcaagagca gcgggataga
      901 gagcacaccg gccagagtga tcgccccacg caccccggcg aaagacgcga tcaggatttc
      961 tcgtgtggtc cacgaaccaa actccatcgg cttcttcttc aggaagcggt tgctgaactt
     1021 tttcatcgtc cacagccagc cgaaacggac cagcatcagc gccgcatata tcagaataat
     1081 atcggtaaac agcatccaga tttcgacgtt agggtcgatt tctgccgcca tcagcgacgt
     1141 ctccagaata cccggcagct gcagacctaa cagcaggaac accatgccgt taaagacaaa
     1201 ttccagcatc gcccaggtgc tgtttgcgcg caggcgcatt gccagcggcg cacggcgcat
     1261 cacaccggag cgggtgatgg tcatcccggc ggcaaccgca gcgaggatgc cggaaacgcc
     1321 aatatgttcg gcaatcagat aagaagcgaa tggcagcaag aacagcagaa cgatctgcgt
     1381 cgcgggttca tcaccgcccc agcggctgag gaatcgcagc gagcgaccat acagccagct
     1441 caccacaaaa ccggcaagaa taccaccaat ggctactttc ataaattcga ccgtcgcacc
     1501 gccgacggtg aagatcatcg tccccatcgc cactgccacg gcaaacttca acgacaccag
     1561 gccggaggcg tcattcatca acgcttcgcc ctgcaaaatg cccatgattt ttttcgggat
     1621 gcgcccttcg ccaacaatcc cggagagcgc cacagcatcg gtcggagaaa gtaccgccgc
     1681 cagcgcaaag gcggggatca gcggaatgcc cggcaccacc cagtaaataa ggaagccaat
     1741 gccgaccacg gtgaccacca ccagcgccag cgcgaggccg aaaatctctc gaccatgttc
     1801 gagaaattca cgggtcggcg ttttccagcc atcagcgaac agcaacggcg ggataaacaa
     1861 gactaaaaag agttcaggat caaactccac atgcaaacca aacgtcggcc acgccagtag
     1921 cgcaccgatg gcgatttgca taagcggaag cgggatctga aagggcatga cacgagtgac
     1981 caccccggac agcgagacca cgagggtcat tatcagtatg gtgaagaaga tttccatgcg
     2041 ttccctgttt gcgatgtttt tttatgatct gagaaaggcg ttatgcctga attctcattt
     2101 tttcagaata tcgcaaaagc tcacacaatg tgcggagaaa ataaaaacgg gcagccattg
     2161 ccacccgttt tcagaatccg atgaaagatt agatagccca gccacccgca tagaaggtca
     2221 ccagcgccac ggcgataacg accgtaccga tgttcaactt gcgccattcg ccggaaacca
     2281 gacgaccaat caccagagtc gcgaagccga tcatgatgcc tgttacgatg ttacaggtca
     2341 gcacgatgaa taccgccgta accagacccg ccatcgcatc aacaaaatca gcaaagtcga
     2401 ttttcgccac gttgctcagc atcagcaggc caacgtacat cagcgccgga gccgttgcat
     2461 accccggaac gaggtaagag agcggagaga ggaacagaat cagcaggaac agcacgccaa
     2521 cggtgatagc cgtcaaaccg gttttaccgc ccgccgccgt acccgccgca gactcgatgt
     2581 ataccgctgc cggagcagca cccaccaggc cagagaaaac gctgctcatg gagtcagtgg
     2641 tcagtgcttt cccaccgtcg atgatctgcc catctttatc cagcaggttc gcctggccgg
     2701 cgacggcacg gatagttccg gtggcatcaa atactgccgt catcaccagc gccagaacgc
     2761 ttggcaggac tacaggattc agcgcgccca taatgtccag gctgccaatc agtgaattgc
     2821 cgttttcatc gctcaatgaa ggcatgccga aaacgccgga gaaatggacg ttaggatcga
     2881 agatcaaacc gacaattgag ataccgataa tggtcagcag aatgccacca gggactttca
     2941 gtttttccag gccgatgatc accgccagac ctaccagtga cataatcacc gggaaggtcg
     3001 cgaaatcacc cagcgcaacg ggcagaccat caagcggttt tttaatcacc agaccaacac
     3061 cattagcggc aatgagcagc aggaacagac cgataccgat ccccgtgccg tgcgccacac
     3121 cgtgcggcaa gttgtgcaaa atccagctac ggatacccgt ggcagaaatt accgtaaaca
     3181 gcacacccat caggaacacg gcacccagcg cgacaggtac gctaatatgt tgccccagca
     3241 ccaggctgaa tgcggtaaac gccgtcaggg agatggcgca accaatcgcc aacggcagat
     3301 tagcccacag acccatcacg atagaaccga gtccggcaac cagacaggtt gcaacgaaaa
     3361 ctgccgcagg cgggaagcct gctttaccca acatacctgg aacgacgatg accgagtaga
     3421 ccatcgccag aaacgttgtt aacccggcaa ccacttcctg acggacagtg cttccacgtt
     3481 gtgaaatttt aaaccaggcg tcgagtgaac cgccggtacg cgctgatggc gtagacataa
     3541 gaaaacatcc cctgagagtt taattttcgt caagatgcgt ggcggacaat cccctgccag
     3601 cgaaacgtta tcttcctgct ccaggtttgc gacaatttac tgcgtcgcaa aaaagcaaac
     3661 gtttagctcc gcgcatacaa aacgggtggt aaaaaaagca aacgattatc cggcgatcat
     3721 agcggctttt tcaaccgaag tttgctgctt ttttctcttt tattgtcttg acgatgaaaa
     3781 aacaggcaat tgatgcgcaa acgttatcgt cggtgcgcaa gattgcagcg ttatcgagcc
     3841 gtataccgtt caggattgcg ataaaaaatc gtctgagatg acaaaaatat tgcggttaaa
     3901 aagtatactt tacactggcg aagaaacgtc ggtgacatac aattaaagca tcaacaccaa
     3961 ccggaacctc caccacgtgc tcgaatgagg tctgttgacg tcgggggaaa ccctcctgtg
     4021 taccagcggg atagagagaa agacaaagac cggaaaacaa actaaagcgc ccttgtggcg
     4081 ctttagtttt gttcatcttc cagcaagcgt gcgccggtac cttcttctcc taagcggtcg
     4141 cccgggttac gcaacgggca atcactgcgc gaaaggcagc cacaaccaat acatccgtcc
     4201 agttcgtcac gcagcgccac taaggtatga atgcgccgat ccaactcttc tcgccattgg
     4261 gacgaaagct gtttccactc tttcgcactt aacgtatgcc cttctggcaa cacgccaaac
     4321 gcttcaccaa tggtcgccag cggaatgcca atacgctgag caattttgat aattgcaaca
     4381 tatcgcaaca catcacgttt atatcgccgc tgattgccgc tgttacggat actggtaatc
     4441 aaccctttac tttcatagaa atgcagcgcc gataccgcca caccgctgcg tttcgccact
     4501 tcgccggggg ttagcagcgc tttaatgcgg ggtaatttct tttccataaa tcgctttacc
     4561 tcaagttaac ttgaggaatt atactcccca acagatgaat taacgaactg aacactgaaa
     4621 agaggcagat ttatgtccca tcagaaaatt attcaggatc ttatcgcatg gattgacgag
     4681 catattgacc agccgcttaa cattgatgta gtcgcaaaaa aatcaggcta ttcaaagtgg
     4741 tacttgcaac gaatgttccg cacggtgacg catcagacgc ttggcgatta cattcgccaa
     4801 cgtcgcctgt tactggccgc cgttgagttg cgcaccaccg agcgtccgat ttttgatatc
     4861 gcaatggacc tgggttatgt ctcgcagcag accttctccc gcgttttccg tcggcagttt
     4921 gatcgcactc ccagcgatta tcgccaccgc ctgtagtttt attgcccgcg cgttaactcc
     4981 cgcgcgggta attgctccat ccattgcata aacacctgcg gcggcatcgc cttcgcaaaa
     5041 aaacatccct ggcaatagcg cacgccgcgt ttgcgcagcc agttaacctg ctcctcagtt
     5101 tcgacgcctt cagcgatcgt ttttaacccc aggctgtgcg ccagctcgat gatgtgttcc
     5161 gcaatcaaat gactggtttt gtgggtggtc agcgtttcaa caaacgattt atcgattttc
     5221 aaaatatcga cattcaatga tttaaggtta tgcaagttag agtagccaat accaaaatca
     5281 tcaattgcca cttcgtaacc agcctggcgg aaagccagaa taatcggcgt cattttttcg
     5341 acatcaagaa atgcatgctc agtcacttca aatttaattt gctgcggacg caccgcgtat
     5401 tgctctgttt tctgattgat tcgcgctatc aaccg
//
LOCUS       DAJTNA010000228         5424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTNA010000228 DAJTNA010000000
VERSION     DAJTNA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.233"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            221..871
                     /gene="inaA"
                     /locus_tag="OO161_003742"
                     /pseudo
     CDS             221..871
                     /gene="inaA"
                     /locus_tag="OO161_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(925..1179)
                     /gene="yfaE"
                     /locus_tag="OO161_003743"
     CDS             complement(925..1179)
                     /gene="yfaE"
                     /locus_tag="OO161_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5464632.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(1179..2309)
                     /gene="nrdB"
                     /locus_tag="OO161_003744"
     CDS             complement(1179..2309)
                     /gene="nrdB"
                     /locus_tag="OO161_003744"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5464633.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFTSNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(2456..4741)
                     /gene="nrdA"
                     /locus_tag="OO161_003745"
     CDS             complement(2456..4741)
                     /gene="nrdA"
                     /locus_tag="OO161_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5464634.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLIMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         1338 a         1280 c         1413 g         1393 t
ORIGIN      
        1 attcatcgct gtatccgcct gattaaattc acattttatc tgctgggcgc actattagtg
       61 gtagtggctg gtggaatcta tgcgctgcaa atgaagggta aaagcggtgt caagctagta
      121 gtggcaaaag aaattcatta atacgacacg tttcattaag attttcctca ggtaaccacc
      181 tatagtcatt gtcgttaata aaaacgacag gcgatagggt atggcagttt cagcaaagta
      241 cgacgaattt aatcactggt gggcaacaga gggcgactgg gttgaagagc ctaactatcg
      301 ccgtaatggt atgagcgggg tgcaatgcgt cgagcgcaac ggcaaaaagc tgtatgtaaa
      361 gcgcatgacg catcatctgt ttcattctgt acgttatccg ttcggccgac caacgattgt
      421 ccgtgaggtt gcggtaatta aataactgga acgggccggt gtcatcgtgc cgaagatcgt
      481 ttttggtgaa gcggtgaaaa ttgagggtga atggcgagcg ttgctggtga ctgaagatat
      541 ggcggggttc atcagcattg ctgactggta tgcccagcat gcagtatcgc cttattctga
      601 cgaagtacgg caagccatgt tgaaagcagt ggcgctggcg tttaagaaaa tgcatagcat
      661 taatcgtcag catggctgtt gttatgttcg ccatatctat gtgaaaacag aaggtaaggc
      721 agaagctggt tttcttgacc tggagaaaag tcgtcgccgc ttgcgtcgcg ataaagcgat
      781 taatcatgac ttccgccagc tggaaaaata tctggagcca atccccaaag cggactggga
      841 gcaggtgaaa gcgtattact acgccatgta atcgttgtag gccagataag acgcataagc
      901 gttgtatctg gcactacaac caattcacat ctcgatttca atatcgcctt ttgcccggca
      961 acaacagggc aaaatttccc ccggctgaat aaaggctaac ggttcggcaa tccagtcaac
     1021 ttgacctgca accaggcgtg tgcgacagga gccgcagtaa ccttcgcgac actggtactc
     1081 aaccgccaca ttgtgggatt ccagcgctgc cagaagggaa gggtgttcat cctggcacag
     1141 cagttgtgtg ccagtgatgc gcagggtaac gcgggccatc agagctggaa gttactcaaa
     1201 tcgtcggtgt ccacttccga gtcaatctgc ccgaccagat aagaactgac ttccacttcc
     1261 tgcggagcaa cctgcacgtt atcagacacc agccaagtgt tgatccacgg gatcgggttg
     1321 gagcgcgtct ggaacggcag atccaaaccg actgcctgca tacggatatt ggtgatgtat
     1381 tcaacgtact ggcagagaat gtctttattc agaccaatca tcgaaccgtc gcggaacaga
     1441 taatccgccc agtctttctc ctgttgagct gcctgaacaa acaggtcata gcactcctgc
     1501 ttacactctt cggcaatttc cgccatctca ggatcgtccg cgccgctgcg cagcagattc
     1561 agcatatgct gggtgccggt caggtgcagg gcttcgtcgc gggcaatcag gcgaataatt
     1621 ttggcgttgc cttccatcaa ttcgcgttct gcaaaggcaa aggaacaagc aaagctgacg
     1681 tagaagcgga tcgcttccag cgcgttaacg ctcatcaggc aaagatacag ttttttcttc
     1741 agctcgcgca ggctaacggt tacggtttta ccgttaacgg tgtgggtgcc ttcgcccaga
     1801 aggtgccagt agctggtcat ttcgatcagt tcatcgtaat agctggagat cccttccgca
     1861 cgtttctgga tctgctcgtt ggtgacgata tcgtcaaaca caatagacgg atcgttaacg
     1921 atattacgaa tgatatgagt ataggaacgg gaatgaatcg tttctgagaa cgcccaggtt
     1981 tcgacccagg tttccagttc cggaatagaa ataagcggca atagcgccac gttcggacta
     2041 cgaccctgaa tggaatccag cagcgtctga tatttcaggt tgctggtaaa aatgtgtttt
     2101 tcgtgctccg gcagcgcctg gtaatcgata cggtcgcggg agacgtcaac ttcttccgga
     2161 cgccagaaga aagagagctg cttttcgatc agcttttcga agatgtcata tttttgctga
     2221 tcgtagcggg ccacgttgac cggctgacca aagaacatag gttctttgag ctgatcattt
     2281 ttcgtctgtg aaaaggtggt atatgccatg agtgtgtcct gttgggagtt taatgccgga
     2341 taaggcattt tacgccgcat cctgtgcctg atgcgacgct ggcgcgtctt atcaggccta
     2401 caaatccgag ccgtaggccg gataaggcgt tcacgctgca tccggcatct caatatcaga
     2461 tcttacatgc gccgctttcg cagccatcgt cctggattga cggtaccaga tcgtcctgcg
     2521 cgtcttcagc gccgtcacgg gtgttctgat aatacagcgt tttgaccccg aatttgtagg
     2581 cggtgagcag gtctttcagc aactgctgca tcggcacttt tcctgacggg aaacgtgacg
     2641 gatcgtagtt ggtgttagca gagatcgact gatcgataaa tttctgcatg atacctacca
     2701 gttgcaggta gccatcgtta cccggcattt cccacagcag ctcataggcg tcgtgcaggt
     2761 gctcgtagtc cggcaccacc tggcgcaaaa taccgtcttt cgacgctttg atgctgacgt
     2821 aaccgcgcgg cggttcaata ccgttagtgg cgttagagat ctgcgaagag gtctcggacg
     2881 gcatcagggc agaaagcgtg gagttacgca ggccgtgcgt tttgattgat tcacgcaatg
     2941 cttcccagtc gtaatgcagc ggctcattag cgatggcatc cagatctttc ttataggtat
     3001 cgatcggcag gatccctttc gcgtaagtgg tttcgttaaa ccacgggcac gcgccttgct
     3061 ctttagccag ctcattagac gctttcagca ggtaatactg aatcgcttcg aaggttttat
     3121 gcgtcaggtt gttggcgctg ccgtcggagt agcgtttacc gtgcttcgcc aggtagtaag
     3181 cgaagttgat cacaccaata cccagcgtac gacgacccat cgctccacgt ttggcggccg
     3241 ggatcgggta atcctgataa tccagcagcg cgtcaagtgc acgaaccgcc agaattgcca
     3301 gctcttccag ttcatccagg ttattaattg cgcccaagtt gaaagcagac agcgtacaca
     3361 gcgcgatttc accgttctcg tcgttgacgt cgttcagcgg tttggtcggc agggctatct
     3421 ccaggcacag gttagactga cgcactggcg cgatggccgg atcaaacggg ctatgggtat
     3481 tacagtggtc aacgttctga atatagatac gaccggtaga cgcacgttcc tgcattatca
     3541 gcgagaacag ctcaacggct ttcacacgct gcttgcggat gctgtcgtct ttctcatatt
     3601 tggtatacag acgttcaaac tcttcctgat cggcgaagaa cgcgtcgtac agccccggta
     3661 cgtcggacgg gctgaacagg gtgatatctt cacctttcag cagacgggta tacatcagtt
     3721 tgttgatttg taccccgtag tccatatgac gcacgcggtt gccttccaca ccacggttgt
     3781 ttttcaacac cagcaggctt tccacttcca gatgccacat cgggtagaac agcgttgccg
     3841 caccgccgcg cacaccgccc tgagagcagg atttcaccgc tgtctggaaa tgtttgtaga
     3901 acggaatgca gccggtatgg aacgcttcac caccgcgaat cgggctaccc agcgcacgaa
     3961 tacgcccggc gttgatgccg atcccggcac gctgggaaac gtatttaaca atcgcgctgg
     4021 aggtggcgtt gatggaatcc aggctgtcac cgcactcgat cagtacgcag gagctgaact
     4081 gacgagtcgg ggtacgcacg ccggacatga ttggcgtcgg cagcgaaatt ttaaatgtgg
     4141 aaaccgcgtc gtaaaaacgc ttcacatatt gcaggcgcgt ttcacgcggg tagttcgaga
     4201 acaggcacgc ggcaaccaga atataaagga actgggcgct ctcatagatt tcgccggtca
     4261 cgcggttctg taccagatat ttgccttcca gctgcttaac ggcagcataa gagaaggtca
     4321 tatcacggtc gtgatcgata aacgtgtcca tctgcttgaa ctcttcttcc gtgtagtctt
     4381 ccagcagatg attatcgtat ttgcccatct cgaccatttt caccacgtgg tcgtacagcg
     4441 caggcggctc aaactggccg taggcttttt tacgcaggtg gaagatcgcc aggcgcgcgg
     4501 cgagatactg ataatccggc gcatcacggg agatcaggtc tgcggcagcc ttgataatgg
     4561 tttcgtggat gtcagaggtc ttgataccgt cataaaactg aatgtgggag cgcagctcga
     4621 cctgggaaat cgaaacgtta tgcagtcctt ctgccgccca atccagaacg cgatggattt
     4681 tgtcgagatt gatgcgctct gtgctaccgt cgcgctttgt caccagcaga ttctgattca
     4741 tgtatgtcgt acctgttttt ggaattcttt ccgcaatagt ttcatgcaga ctacctgtag
     4801 tacgggaagc tgcttagaac actatatata gggggtatgt ttgggattca ctgcaagata
     4861 gtgtgaaaat ggccctcttg caagtgcata actttgtgga taactcagga aggaaaaagt
     4921 ggctttcgcg caccttaggt cagacaaggt gtccgggaaa gtcaatggga agagaaaaat
     4981 ttgttaaaaa taactgttcg gcgatttctt gaacggcaga ataggttgga taaattgata
     5041 tagatggttt attaatcttt ttgctaatga ttgcaaaaat tgatctaagc acgagtcatc
     5101 acataatctg atgtatcagt tgtggtcaat atataagcga atgtaagcga ttgttgatgt
     5161 cgaaatggtt gttttctatt cggttattgt taaatgcgaa gccattttgt taatttacct
     5221 tatattataa aaaaattatt cattacttta atttagttta aatataactg gtatccctta
     5281 aggttattaa cttgaattat gacgcactga tattattcat caaataataa caaaatagcc
     5341 attgcatcgg gttggacaat ttacaaaaga aagataaccc atattgtata atgcgtttaa
     5401 atatgcggat tatctttcta cgca
//
LOCUS       DAJTNA010000229         5360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTNA010000229 DAJTNA010000000
VERSION     DAJTNA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.234"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(912..1853)
                     /gene="fabY"
                     /locus_tag="OO161_003746"
                     /gene_synonym="yiiD"
     CDS             complement(912..1853)
                     /gene="fabY"
                     /locus_tag="OO161_003746"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5464635.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1898..2335)
                     /gene="dtd"
                     /locus_tag="OO161_003747"
     CDS             complement(1898..2335)
                     /gene="dtd"
                     /locus_tag="OO161_003747"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5464636.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2332..3204)
                     /locus_tag="OO161_003748"
     CDS             complement(2332..3204)
                     /locus_tag="OO161_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5464637.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(3198..3797)
                     /gene="yihX"
                     /locus_tag="OO161_003749"
     CDS             complement(3198..3797)
                     /gene="yihX"
                     /locus_tag="OO161_003749"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5464638.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3938..4825)
                     /locus_tag="OO161_003750"
     CDS             complement(3938..4825)
                     /locus_tag="OO161_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5464639.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4815..>5360)
                     /locus_tag="OO161_003751"
     CDS             complement(4815..>5360)
                     /locus_tag="OO161_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5464640.1"
                     /translation="FGIVYISSQLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQ
                     KSLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFG
                     TKVRYTGLSFAYQFSAIVGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLI
                     EPTDEQETASYRYIREQSHEN"
BASE COUNT         1446 a         1354 c         1253 g         1307 t
ORIGIN      
        1 tccgctattg ccagtacacc tcattctgat aaatgaagaa aaaccgtatt catcgccatt
       61 accaccacct tacctatatt actcattaat gtatgtgcga aaattacgcc ggaaaatcat
      121 taaaaaaaag cggatttttg catgttgttt tgtttttgcg aggctttttc cagaaataat
      181 aagagtgttg cagttgtttc atttattact caaaaatggt ttagttatcg cccaggatga
      241 attatgggtg ctatgaatat attatattca gagatattta tttgcataat ctgaatggtg
      301 tagccgaaaa acgtcagcat cctaacagca ccaggattta aggtgaaatt aatctttcat
      361 cacagaccgg tcagtactgc caccaaccgg tctttacttg ctcatcgtta taattttgtt
      421 ggtaacagtt cgggaaagtt gtttttctaa attaacaaca gaataacttt cttgtttaat
      481 gagattaata ttggcaacca ttatagcctc cttcccacgg tttaagagca aacatacaac
      541 ataatgattc ataacataca gagcagtaaa tgaatgatac tgacaatgat gccagcccat
      601 tatctttgca taatgtttgt gagctataga ttttaaaaat atttatgagg aaagtatggc
      661 accagaggag gttttgacta tcaaaaatcg ctcaaacctg aactaagatg atgagttaag
      721 cattatctgc gataattgcc actcagctct acaaataata tttaaatgct cctgaacagt
      781 gatgtagcaa gacacgtatg gtgccaacga agaataaaaa tttatagagt tcttattcaa
      841 tattaccggc atagacaggg aatgatgtaa atgaccggaa tgagagaggc agcaccgccc
      901 ctctccataa gctactcttc ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg
      961 ggcagaacga tatacgtgcc ttcaaacact gcacccggcg tctcgtcgcc aaagatttca
     1021 acttgcatct gcacccgtgc ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag
     1081 gcaccaaggt cggctaccgc atgaggttta ccgctaatcg gcttgctgta gcggatatgc
     1141 gcatcggcca gaataatcgt tccgccgagg tggcgttcgc gcagcatcag ccagataagc
     1201 ccccaaccgg tgagcgttgc cagcgagaat aaactcccgg caaacagcgt atggtgcgga
     1261 ttctgattac cggtttccgg catggtggtg ataaattttt gcccggtata ttgctgaatg
     1321 cgcacgccca ttttttcact aagcgggatg tgttcgtacc acgcctgttg cagctgcgcg
     1381 caccagtcgc cgcgatgcaa aatatcatcc agagtggcaa caggtttaat catcaaaaaa
     1441 tggcgaatcg gtgtggtggt tggtgtggtg atttctccct gattaacaaa ccccagcttg
     1501 gcgaaaaact ccaccgcgtc ttcacgggcg ctacaggtca cgcgcttaac gccttcctga
     1561 cgcgccaccg actccagggt catcgccatc aacgtcccca gccctttgtc ctgcacgtcg
     1621 ggatgaacgg ccataaagcg aatggacgct tcattgtcgg cattaatata cagtcggcct
     1681 accgccacca gattcccctg ctcgtcgacg accatctgat gatgcgccat cgcatcccac
     1741 gcgtcgcgtt ccgaaccttt tggttgatgc aggggcttac gcaacatttc ccagcgaaac
     1801 tgatagtaac gctctaactc ttcttctgtt tgtggaaccc gaaggtgata catagcgata
     1861 ctctctcttg tttcccgtaa caaccctgga agctggctca tacctgcaac cagaatgtca
     1921 cggggccatc attgaccagc gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt
     1981 tcatctcttg ctggcggcag cgttcgacga aatagtcata taacgcctct gcgcgatccg
     2041 gtgatgcgcc tttggagaaa cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa
     2101 actgggaaac caccagtaca ctgccgcccg cctgttgcac gttgagattc atcttgcctt
     2161 cggcatcgct aaagatgcgg tagccgagca cacgctcgca cagacggttt gctttctgtt
     2221 cgtcgtcatc cttttcgaca cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca
     2281 cttctccctc cacggtgacg ctggcacggg ttacgcgttg aattaatgca atcatggttc
     2341 gtcgtcttct tcttgttcag ctgcttgttt tagtttgcgg tattccccga gagtgacagt
     2401 aatttccgcg ccaagcaaga cgatacacca tgtccagtag acccaaacaa agagaatggg
     2461 gatcaccgcc agcacaccgt aaatgagctg atatgacggg aacatggtga tataaagcgc
     2521 gaaacctttc tttcctgctt cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc
     2581 gcggttaggt acgcggatgg taggaacaat gctgtacagc aaccagaagg agatccacga
     2641 caacagcaac ggaaaaatac gcagcacgtt gtcgatgaca gtattgagat cgctcgccca
     2701 gcgcagggag agcaaatagg aactgatcgc cagactggcc cctgccagca gcggccccag
     2761 cgttaaaatc atccagtaca cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg
     2821 ccagatggta ttcaacgcgc tatcgatgga gtacatcaac aataacgcgg tgacgatcag
     2881 cccgcacgcc ccaacggcgg tcatcttgtt ggaattggca acaaattgtt cgatataccg
     2941 ctgaataaca tcgccagtag caggcagaaa gttggcaaaa ataaagtgac gtaactgaat
     3001 gctgacgtcg gaaaacatgg gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg
     3061 cactaatgag agcaacgaca cataggcaag gttacctgcc agggttgtca tgttgtcctc
     3121 atcaatacgt tgccagagta gttttagcca ggcccatagt ggacgggtac ggtgcctggc
     3181 tttgtcctga atggttttta gcataacacc ttcgcgaaat agtccgggat ggtggtttta
     3241 tctttcacca gaatactggt aatgcccagc tgattggctc cttctatatt atcggcgtta
     3301 tcgtcgaaaa agaccgtatc gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa
     3361 attcgtgctt caggtttgcg catccccaga tcttgcgaca gatagatatg gtcagcagca
     3421 tcacgaattt ccgggtattc ttccggccag aaggtggtat gcaggcggtt ggtattggaa
     3481 agcaccacca cgcgatgccc ctgctcacgc agtttatgca tgatggcgat cacttccggg
     3541 cgcagcgcaa caaacaccgc ctgccagccg tgagagaact gctcgtagct tagcggtaga
     3601 gccatctcat gacacagcgc ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc
     3661 tgatgaaacg cctcccccat atgaaaactc ttcttaagcg tagccagtgg aacacgcgtt
     3721 aaatcgctcc aggctcccag cacacggtta aagtcgatat cgacaatcac attacctaaa
     3781 tcaaagatat agagcatttt tgcctccttt cgcgccacaa gaaattaact gtagcgggaa
     3841 aggatgggtt tggctatgtg gcaataaggg taatcttgtg atgctgattg cgaaaaagca
     3901 gagtaatttc actggctttt tcgcaataga tgaatggtta agattcaggt gtaaattgcc
     3961 aacgagtttc gatgaccagc ggcctatctg gtgtcaccaa ttccggcgtt agatttattg
     4021 cgttaggtgc ctgagtcagc gggttaatac aggtggcatc gggctgttta tcaaatacca
     4081 ccagactgtt tgcagaagag atcatggtca tcgcgagttt accaggccac agtaacttaa
     4141 ccttcacccc gttataaaaa ccaaaacagt catcccaggg accggcttga ggagagatac
     4201 gatttccagt tggcagttca tcactacccg cttcttcttg ccagtccgca tcaaaaagca
     4261 cctgcaacga ttctgtattt tgcggagtca gctttttcgc aaaccacgga tgccagccag
     4321 cggaggccgg gaatgtatcg gcatatgaat ggacttccag ttgcaaaacc agcgcatcgt
     4381 tctccagcaa aaaagtctga atcacttccc cttgccaggg ccacggtgaa gccaggggca
     4441 tacgtaaagt cagggaatct gctgttttgt cgatgatttc ccacgttgag taacaagcca
     4501 taccatgcaa cgcatgtggt ggtttattgg cgggtaaaga ataacattgc ccaccagcat
     4561 tcagcgtcgc attccccagt cgccctgccc acggcaccat tgggaaacag ccatactgaa
     4621 aagctctgcg ctgcggctgc cattggcgta atacttcaga accaaatgcc tttaacgagg
     4681 ttatcctggc accatcatgc ggataaatct gcaggtgaat attgcctttt tccagcgtca
     4741 ctaactctcc tacaggccca ggccatggcg cgtcaaacgc ctgttgtagc gccaggtaat
     4801 gctcgttttt tcgttcagtt ttcatgagat tgttccctga tgtagcggta agaagcggtt
     4861 tcttgttcat cggtcggctc aatcagccag acgcaaacaa aggaaagaac tgagacagca
     4921 aataaaaaca gagcgacata ccacggtttt cctcccccta gcgccagcaa acttgaggcg
     4981 ataagggggc ttagtccgcc aacaatcgcg gaaaattgat aagcaaaaga aaggccggta
     5041 tatcgtactt tggtaccgaa catgcgggtg aaaagtgttg gctgcacggc aaacatcatc
     5101 gtcggaccta agttgtagcc gaggatcata ctgcaccaga tgatgagcgt acttttgcta
     5161 tccagtaata agaagaaagg aaacgcgaac aggacacaaa atcctgctcc cgacaaatac
     5221 agactttttt gacctatacg atctgataac catccaacca atggacaact gaaaataccg
     5281 atcgccgagg caatcaacat acccgtcagg ggaatatctc gcgacaatgc taattggctg
     5341 gaaatataga caataccaaa
//
LOCUS       DAJTNA010000230         5344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTNA010000230 DAJTNA010000000
VERSION     DAJTNA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.235"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            30..1382
                     /locus_tag="OO161_003752"
     CDS             30..1382
                     /locus_tag="OO161_003752"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5464641.1"
                     /translation="MEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNHEA
                     IDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
                     GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTRYKEKVKYWMTFNEIN
                     NQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVQTGKSINPEFNIGCMIAMYPIY
                     PLTCVPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILAS
                     GCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLN
                     WFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVDLIG
                     YTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI
                     "
     gene            complement(1439..1657)
                     /gene="marB"
                     /locus_tag="OO161_003753"
     CDS             complement(1439..1657)
                     /gene="marB"
                     /locus_tag="OO161_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5464642.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1689..2072)
                     /gene="marA"
                     /locus_tag="OO161_003754"
     CDS             complement(1689..2072)
                     /gene="marA"
                     /locus_tag="OO161_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5464643.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(2092..2526)
                     /gene="marR"
                     /locus_tag="OO161_003755"
     CDS             complement(2092..2526)
                     /gene="marR"
                     /locus_tag="OO161_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5464644.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2738..3379
                     /gene="marC"
                     /locus_tag="OO161_003756"
     CDS             2738..3379
                     /gene="marC"
                     /locus_tag="OO161_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5464645.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRNSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINDH"
     gene            complement(3417..4606)
                     /gene="ydeA"
                     /locus_tag="OO161_003757"
                     /pseudo
     CDS             complement(3417..4606)
                     /gene="ydeA"
                     /locus_tag="OO161_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
     gene            complement(4756..>5344)
                     /gene="yneK"
                     /locus_tag="OO161_003758"
     CDS             complement(4756..>5344)
                     /gene="yneK"
                     /locus_tag="OO161_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5464646.1"
                     /translation="IEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWSNSEVH
                     RWFVNKDPNLITSILDITDGELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESP
                     HFQDFFLFFANMLLHYHQFMNPNESDLNDMLMPASLSDDKIIKHMARRTLKLFVKNET
                     PPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
BASE COUNT         1440 a         1196 c         1318 g         1390 t
ORIGIN      
        1 tatggggcgg cgcggtagcc gcgcatcagt tggaaggtgg ctggaatgaa ggaggaaaag
       61 gcatcagtat cgctgatgtg atgactgctg gcgctcacgg ggtgccgcgt gaagtgacag
      121 aaggcgttat cgacgggctt aattatccca atcatgaagc aattgatttt tatcatcgct
      181 ataaaacaga tattcagtta tttgccgaga tgggattcaa atgctttcga acttccattg
      241 cctggacacg aatctttccg caaggtgacg aacaggagcc gaatgaagag ggtttacaat
      301 tttatgatga tctgttcgat gaatgcctta agcagggaat ggaacctgtg gtgacgcttt
      361 cacattttga gatgccttat catctggtga caaaatatgg tggctggcga aaccgtaaac
      421 tgatcgactt ttttatccgc ttcgcatcaa cggttttcac gcgctataaa gaaaaagtaa
      481 agtactggat gacgtttaac gaaatcaata atcaggtgaa tttcagcgaa agcctgtgtc
      541 catttactaa ttccggtatc ttgtattcgc cagaggaaga tatcaatgag cgcgaacaaa
      601 taatgtacca ggcggtacag actggaaaat cgatcaatcc tgaatttaac attggctgta
      661 tgatcgccat gtatcccatc tatcctctga cgtgtgtacc caacgatatg atgatggcca
      721 cgaaagcaat gcatcgtcgt tactggttta ctgatgttca tgctcgtgga tattatccgc
      781 aacatatgct gaattacttt gccaggaaag gattcaacct cgatatcaca ccagaagata
      841 acgcgattct tgccagtggt tgtgtcgact ttatcggctt tagctactac atgtctttta
      901 cgacacaatt ttcgcccgat aacccgcaac tggattatgt tgaaccacga gatttggtca
      961 gcaaccctta tatcgataca tccgaatggg gatggcaaat tgatccggca gggctacgtt
     1021 attcactcaa ctggttctgg gatcatttcc agttgccgct gtttattgtc gaaaatggat
     1081 ttggtgcggt tgaccagaga caagctgacg gcacggtgaa cgatcactat cgcattgatt
     1141 actttgcttc ccatattcgg gaaatgaaaa aagccgtagt tgaagatggt gttgacttaa
     1201 ttggctacac cccgtggggc tgcattgacc tggtttctgc cggaacaggg gaaatgaaaa
     1261 agcgctacgg aatgatttat gtcgataaag acaacgaagg gaagggaacg ctggaacgga
     1321 tacgtaaagc gtcgttttac tggtatcggg atctcatcgc caacaatggc gaaaatattt
     1381 gagacttgag gaagatatta cggagatgtc aggctctgtg atcagagcct gacaataact
     1441 acatagcgtg ttgattataa tagggcacgc cgagcgcatc cgacttatca ctgccagtac
     1501 ccatgtgatt taaatcaaac ggtggttgtt cctgcgatgg gggaacaacc acgacattgg
     1561 cacaagaagt aacaactggc tgcgtggttt gttccgcaac gccctgcgcg gaaaagagaa
     1621 taagcgcagc tgctattgcg gatgaaagtg gtttcatgat tgcctcagtg acgttgtcac
     1681 gttatcaact agctattgta atgatttaat ggatgtaaaa agcgcgattc gccctgcata
     1741 ttggtcatcc ggtatttatg tggcggaaca tcaaagtaat ttttgaaggt tcgggtcaga
     1801 gtttgttgcg actcgaagcc atatcgttct gccagataga gtatcggctc gttactttcc
     1861 ttcagctttt gcgcgatttc cgtcatctta cggctgcgga tgtattggcc taatgaatga
     1921 ccggtttctt ttttaaacat ccgttgcagg tgccatttag agtaacctga acgctctgac
     1981 actttctcca gtgacagtgg cgattccagg ttgtcctcga tccagtccaa aatgctatga
     2041 atggtaatag cgtcagtatt gcgtctggac atcgtcatac ctcttttttg tttacggcag
     2101 gactttctta agcaaatgct caagtgttgc cacttcgtcc gccgtcaggt tttttgttaa
     2161 ttcttgatgc aggtcctggc caactaattg atggcattgt tcgcatattg ccgcgccgct
     2221 ggtggtaagt tttaccagta cgccgcgctt atcattcggg ttcggcaacc tttcaaccca
     2281 gcctttacag accaggcgat ccagcatacg ggtcagtgct ccaaggtcga cagacaacac
     2341 ttttttcagt tcaaccggag taatacacgc cgcgcagcgg atagagcaga gcaccttaaa
     2401 ctgtgccgcg gtaatatcca gcggagacag atactcgtta agcagacgat ctttcttctg
     2461 attaaccata tggattaagc gacccaatgg aataatttca ttgaacagat cgctggtact
     2521 tttcacatga gttgccctgg caagtaatta gtcgcagggg gtaatattgc ccaggcaagt
     2581 ataagtcaaa taaatgaatt gaccgatgcc acgttttgct aaatcggttc aagttttggt
     2641 caatgcgatg caaggttagc taaagagtga cactttatcc ttgtttaccc cttatacttt
     2701 tcgctgataa cccagataca ctggataaca accaccaatg ttagatttgt ttaaagcaat
     2761 tggcctgggg ctggtggtgt tactgccgtt agctaaccca ttaacaactg tcgcgttatt
     2821 tcttggcctg gcaggcaaca tgaacagtgc cgaacgtaat cgtcagtcgt tgatggcctc
     2881 ggtgtacgta tttgccatca tgatggtggc gtattacgcc gggcaactgg tgatggatac
     2941 atttggcatt tcgattcccg gtctgcgaat tgcaggcggc ttaatcgtcg cgtttatcgg
     3001 ttttcggatg ctctttccgc aacagaaagc gattgattca ccggaggcga aaagcaagtc
     3061 ggaagagctg gaagatgaac ccagtgccaa tattgccttt gtgccgctag caatgccaag
     3121 taccgctggt ccgggaacca tagcgatgat tattagctcg gcgtccacag tgcgtcagag
     3181 ttcaaccttc gctgattggg tactgatggt tgcgccgccg ctgatctttt tcctggtggc
     3241 ggttattttg tggggaagcc tacgcaactc cggcgcaatt atgcggctgg tgggcaaggg
     3301 tggcattgaa gcgatctccc gtctgatggg attcctgctg gtatgtatgg gtgtgcagtt
     3361 tattattaac gatcattaaa acgtaccatt aaaaaaggcc cgaatgggcc tttcaactat
     3421 tgcgtctgtt cttcgagtgt cactgccagc ggcgaaatat aatgattgac catattaacg
     3481 cggcaaaagc aggcaccgcg cccacataac caatcatcga cattgaccag tgcaaactca
     3541 cctgattacc taccaacgca cccgccccga ttccaatatt aaatatgccg gagaatagcg
     3601 ccatcgcgac gtcggtagca tctggtgcca gcgccagcac tttaacctgc ataccaagcc
     3661 cgatgatcat catcgcgatc ccccagaaaa tactcagcac cccgaggtgt atttcactgt
     3721 tcgccgcagg tagcagcagt gccagacaca ccagcaacag cgcaatcgcc gtactcacca
     3781 acgcagacgc atactgatta cccagtttac cgaaaatcac gctgccaata atgcccgcac
     3841 caccgagtaa taacagtaat gccgtggcaa agttggcgct gaatcccgca atgttttgca
     3901 caaaaggctc gatatagctg tatgccgtgt aatgggcggt gacaaccacc acagttaaca
     3961 aataaatgct catcaatgcc gggcggcgga acaatagcgg gaggcttttc aatgaaccgg
     4021 aatgctcact gggcagtaag ggaagtaact taatcaggca caaaagggtg ataagcgccc
     4081 caataccaat cgcgaagaag gtcattcgcc aaccgaaata ctgccccaca atgcgcccga
     4141 gaggtaaacc taagaccatc gccagtgctg taccggtggc aattaaactc aaggcctgtg
     4201 cccgcttccc ggccggagcc atacggatcg ccagagacgc cgtaatcgac cagaaaatcg
     4261 catgtgcaaa agccacacca atgcgactga tcaccagaac ggtaaagctc cacgataaaa
     4321 acgacagtac gtggctggca ataaacacca caaacaggca gatcagtaat ttgcgccgtt
     4381 caacctgact ggtcattaac ataaaaggca atgacattag cgctactacc catgcgtaaa
     4441 tggtcaacat gatgccgacc tgagcggttt gcatgtgaaa actttgcgca atgtcagaga
     4501 gcaggccaac agggacaaat tcggtggtgt tgaagatgaa ggcggcgact gccagcgtaa
     4561 cgacccgtag ccacgccact ttgcgggaaa cagtgtttgt tgtcataaca gattatcggg
     4621 tgagttgcga aaaagtgatg aaatgatctt aaaacggtta atgatgtgaa tacaatgcat
     4681 ttgtgatctg gatcgcgttt tccgtttctc tgacgctgat tctgttccgc gtcagagagt
     4741 cggtaacatc acggtttaat gacgttcagg gaggtctggc gttttggcgg ttgcgggtat
     4801 aggtggtctg acaggggagc gaggcctgtt tttcaccagg tcttcgtgag taacttttgg
     4861 cggcgtttca ttttttacaa acagtttgag ggtcctgcgc gccatatgct tgataatttt
     4921 atcatcactt aatgatgctg gcatcaacat gtcattcaga tcactttcat tgggattcat
     4981 aaattggtga tagtgcaata acatattggc gaaaaataaa aaaaagtcct gaaaatgagg
     5041 agattcttgt tctgctaaaa gggataaaaa gcagatgcag acttcgggta gtacatgttt
     5101 gttagaacct aatgaatcca tcaggctttg caataaatcc tcttttaatt cgccgtctgt
     5161 tatgtcgaga atggaggtga tcaagttagg atctttattg acgaaccatc tgtgaacctc
     5221 agagttactc cataatcggg ggagtatgac aaaattaccg cgagagataa aattcaggac
     5281 aagcaaatca aactggcgta agtaatctgc agcggtatgg tgatccggag gcaggggttc
     5341 aata
//
LOCUS       DAJTNA010000231         5202 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTNA010000231 DAJTNA010000000
VERSION     DAJTNA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.236"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..120
                     /locus_tag="OO161_003759"
     CDS             <1..120
                     /locus_tag="OO161_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR5464647.1"
                     /translation="SDWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(170..1918)
                     /locus_tag="OO161_003760"
     CDS             complement(170..1918)
                     /locus_tag="OO161_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR5464648.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADETQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(1918..2988)
                     /locus_tag="OO161_003761"
     CDS             complement(1918..2988)
                     /locus_tag="OO161_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5464649.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            complement(2978..4420)
                     /locus_tag="OO161_003762"
     CDS             complement(2978..4420)
                     /locus_tag="OO161_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5464650.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFNFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTACFSLLEK
                     HIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYT
                     LAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGL
                     ALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4431..4877)
                     /locus_tag="OO161_003763"
     CDS             complement(4431..4877)
                     /locus_tag="OO161_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5464651.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1313 a         1342 c         1313 g         1234 t
ORIGIN      
        1 tctgactggg ataaagagca acttcaggac gcaaactcaa gctggatggt cgaagactct
       61 ttcccgagag cattacgcga gtacaacgaa atggttgatg actataatag cctgcgttaa
      121 acgtttttga tgataaatgc agtgtcggat gcggcgcatc cgacacagtt caccccttgt
      181 gaagtaactc cataaatgcc tcatatcctg ttaacccggc aataacctct gccggatgct
      241 gacacaatat gcttgccaga tagctaaata tttttagctc atgacgcgca tcggcggcgg
      301 cgcaggcgat caagacatgg ttaatgactt cgttattcac ctcaactggt tgggcgaggg
      361 taataaaaaa tccacgaaaa cgtcgctcct gttcgctcca gcaatgtggg atggcgaggc
      421 gattaacaat caggttttca ccttcgcctt cgcgggcgat gatgcgttgt gtttcatcgg
      481 cggtaatatg gtgttgtgcg actaattgcg cacaaatttg ccgggtaata tgttgccagc
      541 tttcaccgcg tacattcgaa taatgaaaac ttccgggggc agagaaaaaa cgctccggtt
      601 gctggcgaat aaatgccgtc gccagaaaat gttttatttg ttcgataccg gcagccgtaa
      661 tgatattttt tacggtgatg taattattta ccgcgtcttc cagtaaataa tggctattgt
      721 taatgatcaa taacggctca atctcttcgc gaatggcaac aagttcgctt aagctacggg
      781 caataattac ccgacaattt aaaacatcgc gctcaatggc gagctgatta atagtggcaa
      841 tcgcgttctg gtccgagagc aaaatgatcg gctggcgttc gttttgatgc cgctccagcg
      901 cacaggcaaa atacaaaccg atcagatcgc tgtcgaaaag gggaatatcg agctgttcac
      961 gcagtagcgt aataaagtgc agactcatat caaaggccgc aggccaggct gctttcaggt
     1021 tattcatgct actttggcgg tgctcagcaa tccacaccgg tgcggcaagg caacgtaata
     1081 aatgcccggt aatattctca accaactgcg tatcaataat attcaatgac gcgatgcctg
     1141 gcaccctttg cagcaacccc agcacatgat cggctgaaat tacctgcgct tgctgatgct
     1201 gtttctctat caaacctgtc agggttctta cagcgttatc accaagaaac atgccgcttt
     1261 gttcaacgat ttgttttatc tcgtcaccgg gatattgcgg ccactcatcg gtcagttgat
     1321 tacgcagggc gtaaatcgcc agaatcaggc tggagagata atcaccctgc atgagcggcc
     1381 agcggtgttg gttgtcgcag gccgtggata aatgctgaag gttgtctcgt gttacgcccg
     1441 ccagcggaat taaaaacgga tctttgcgca gcaagttcgc cagcaagata acgcgcttct
     1501 cttctgtctc atcaatgaaa tggcccaaac cggggcggct ggccaggcaa caagcgcgtt
     1561 cataacgctg ttttaaccgg ggcagacgct ctgcgaccca cgtttctggc aaattaagcg
     1621 ccgaggcgag ttgcgcacgg ggagtaaagg tattcagtaa taacagtgcc agcagccgat
     1681 agtcgttatc gtgcttttgc agcaactgaa aaaagctgcg gcgctcgaag atttccagct
     1741 gatagcccgc actaccactg gcggaaatgc gggctttgcc gttaagggta aagttgagat
     1801 agtcaatatc acgcaggatg gtcctgcctg aaacgccagt ctgttgcgcc agctcaccag
     1861 gcgtgcgcgg ctgttgctcc agcagatcga caatctttaa ctggcgttcg tttagcatta
     1921 atccacctga cggaactggc taaacgcgtt gactttctcc gcggttaagg tggtcagaaa
     1981 atcccgtatc aacgtgagca gagcatcgta atcggctttt gaaatcatac cgctgttagc
     2041 gtgcagatag cgggttggca ggcacagcgc aacaaccgga cgcccgccgc ccatcacgtt
     2101 gtagcgcccg ccatccgtcg caccggtttt catggtggaa aattgcagcg gtaaatcgtt
     2161 atgtgcggca cagcttttta acgctgctac cagtttctgg ttggggaagt agcgcttgtc
     2221 aaacagcatc agccccggcc cctggcccag tttcagcggg tatttaatgt tatcaatgcc
     2281 cggaacatcg cctgcgacag cggtatccag cacgatcact acatccggtt taatgtgttc
     2341 cgccgaggtt tgtgccccgc gtagcccaac ttcttcttcc acgctgccga cgccatacag
     2401 cgtaatttcg ggattattca ccgtctgcaa tagttcagcc atcattgcgc agccgatgcg
     2461 gttatccagc gccttgccga ccactttatc ttcgccccag caggcaaaat tggcttccgg
     2521 gctaataaaa tcgcccatcg ccacaccgcg tttttccacc tcatcgcgac tgtttgcgcc
     2581 aatatcaatg aacatctcat caaatgacag cggctgttgc ctttgctttt ccgttaacgc
     2641 atgaggcgcg acggaaccaa tcacaccagg gattttcact cccttgtgtg tgcgtatggt
     2701 tacccggtgg ttgagcatcg actgattcca ccagccacca atggtggtga aacgcagaaa
     2761 accgctctcg tcgatgtggg tgaccataaa gccgacttca tccatatgcc cgacaacggc
     2821 aacttttgga cctttattcc ccttacgggc aacaaagctg cccagaccat caaaggtgat
     2881 ctcattaacg caaggttcca gcgtgtttat cagaatgtcg cgaacttcct gttcatcgcc
     2941 gctgacggcg ctggcttcgc acaactgttg cagtaactca atgttcatgg acaagctcct
     3001 gttgcgcggc tttcgctttg cggcgtaacc acaatccttt caggacgata atgaatgaaa
     3061 tgttcagcgc gaggccaatc gccagcacca gataaaacga acccaccggc gacatcaggc
     3121 cgatcagcgg gtcaaagata cccaaaccag gtgccagacg tttgattccg aaagcgatca
     3181 ccagcacgcc ggtaatgcca cccgccagcg tattggcggt aatcatcggc aacggagctg
     3241 ccagagtgta aggtatagcc ggttcggttg cgactgtggc accgaccaca atggcactac
     3301 tggcagcctc tttttcctgt cgggtaaaca gtttgggcgc aatgaacgtc gcaagacccg
     3361 ccgcaacggg cggcatcaac gcgacaacgc cgacgatggc gtaccagtca taaatgtgtt
     3421 tttccagcag tgagaagcag gcggttttat tgatggggcc gcccatatcg aacgccagca
     3481 tcgcaccgac cagaaacgct gctgcaaact tcattgatgg aggaatggta ttcaggaagt
     3541 ggagcagccc gcccatgagg tcggacatca ccggcccgat aacgtagtaa gtgagcacgc
     3601 cgaaaaccag cagagtaacg aacgggatca gcattgaacc gagcagcggt tgtaatgctt
     3661 tgccaaggcg cactttgcgg aaccagaaga cgaaatagcc aatcgccagc cccagcacca
     3721 ccgcgccgag gaatcccgca cccgactggg tgccgagcag cgctttgtcg ttcgccagat
     3781 agcaaaccag aaacgccgga gcgaaagccg gtttatcggc aatcgacgag gcaatataag
     3841 cgcccatgat ggggatcata aagttgaagc caaggtaacc gatggattca actacccagg
     3901 taaacgacgg cgcgcctttc gacatatcgg tatacggcaa accgaactgc accagcatgt
     3961 tggcaagcgc caccaggata ccaccgccga tgacaaacgg cagcgccgca gagacgcccg
     4021 ccatcaggtg gctcatcacg ctgccgcttt gcatttcctg tttgccaagc ttcacgccac
     4081 tatctgcggc aaaaagctgc gagtttgtcg gtaattcgct gaaaatctgg tcgatatttt
     4141 tcaacgcctg ggagatggca atctcataaa ctttcttccc ggtaaaccgc gcgcgatcat
     4201 caccgctcag gccacgcccg gtagcgagaa tgacgtaatc ggcagcggca atctcttcgc
     4261 tggataagcg gttttcaacg ccactggacc cttgagtttc cacttttatg gtatggccga
     4321 gagaacgggc tttctgttcc agggcttccg ccaccatgta ggtgtgagcg atcccggcgg
     4381 ggcagttggt gatcgcgaca atacgtaagg atgactccat cgccacttcc ttatgagctg
     4441 agggtttgat ttaacaaggc aagcacttgg tcggtatcgc cctgttgcag ttgatgaata
     4501 aattccttgt gaataatttt gcgacacagt gtgccgatga ttttgacctg atcctcttcg
     4561 ccgctttgcg gcacgccgag gcagatccag caattgacgt cttcgccatc gctggctttc
     4621 cagtcaatag cctgcgcttt gcgggcgaat aatacgaacg gttgtttaac gcaggcgctt
     4681 ttaccgtgcg gcacggcgac accggaacca aatccagtag agtgcatttt ttcgcgcagc
     4741 agcagggttt gcaggaactg gtgtgagtcg gtgataaaac cgttttgtaa cgctattgtc
     4801 gccaactgct tcagaacgga ataagcgcca ttgccctgaa tattcaggtc aatacagctt
     4861 gcagtaagag ctgccattaa tcggactcca taaaatatat tttaatcaat tgtaatggtg
     4921 gagcgattaa gtgatgagag gcgatatgtc ggtaaacaat gacaatttga gacagagtga
     4981 aaggtcagat ttgcggagta atgcacataa tggttattta aataaaccac atgaatcatt
     5041 aatgggttat tcattatttt tgtgatttac tcacaatgta tcagggaaat ataacttacc
     5101 gggagatgta atatgtttaa tttttcaaat cgaaatttaa aatattgtgc cggaggcatc
     5161 tccggcacat tgggcaatta cggcaggtaa aacacttctt gc
//
LOCUS       DAJTNA010000232         5162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTNA010000232 DAJTNA010000000
VERSION     DAJTNA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.237"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            8..667
                     /locus_tag="OO161_003764"
     CDS             8..667
                     /locus_tag="OO161_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5464652.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            877..2937
                     /gene="ptrB"
                     /locus_tag="OO161_003765"
     CDS             877..2937
                     /gene="ptrB"
                     /locus_tag="OO161_003765"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5464653.1"
                     /translation="MLPKAACIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYAGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(2934..3596)
                     /gene="exoX"
                     /locus_tag="OO161_003766"
     CDS             complement(2934..3596)
                     /gene="exoX"
                     /locus_tag="OO161_003766"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5464654.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWSFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(3620..4276)
                     /locus_tag="OO161_003767"
     CDS             complement(3620..4276)
                     /locus_tag="OO161_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR5464655.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(4378..4608)
                     /gene="holE"
                     /locus_tag="OO161_003768"
     CDS             complement(4378..4608)
                     /gene="holE"
                     /locus_tag="OO161_003768"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5464656.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            4747..5121
                     /gene="yobA"
                     /locus_tag="OO161_003769"
     CDS             4747..5121
                     /gene="yobA"
                     /locus_tag="OO161_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5464657.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
BASE COUNT         1331 a         1272 c         1307 g         1252 t
ORIGIN      
        1 agcaaatatg gctaactggt taaatcaact gcaatccctg cttgggcaaa gcagttcttc
       61 tatctcctcg tctgcggatc agggattggg caaactgtta gtgccaggcg cattaggcgg
      121 actggctggg ctgctggtcg caaataaatc agcacgtaaa cttcttacga aatatggcac
      181 caacgcgtta ctggttggcg gcggagcggt agcgggtacg gtgctgtgga ataaatacaa
      241 agataaaatt cgcgcggcgc atcaggacga accgcagttt ggcgcgcaaa gtacgccgct
      301 ggatgagcgt acagaacgtt tgatccttgc gctggtcttt gccgctaaaa gtgatggtca
      361 tattgatgcc aaagaacgtg cggcaatcga ccagcaattg cgtgaagccg gcgtggaaga
      421 gcaggggcgt gtactcattg agcaggcaat cgaacaaccg ctggatccac aacgtctggc
      481 taccggggtc cgcaatgaag aagaggcgct ggaaatctat ttcctgagtt gcgcggctat
      541 tgatattgac cattttatgg aacgcagtta cctgaatgca ctgggtgacg cgctgaaaat
      601 cccccaggat gttcgtgacg gcattgaacg cgatctcgaa cagcaaaaac gcactttggc
      661 ggaataattc cgctctctgg atagcctgaa gttgactatt tcgggctatc cagaatcgca
      721 cagccatttg aacaggctga ttttctctgt ctgcataaca ttcgatatcc gcgccattac
      781 ctgtcatcat ctaagcaatg actcccctgt ttcgcttgca tccccggtga gttttgccac
      841 ccttaataag atgtttcaac cagaaagaac aataacatgc taccaaaagc cgcctgcatt
      901 ccccacgcca tgacgcttca tggcgatacg cgcatcgata attactactg gctgcgggac
      961 gatacgcgtt ctcagccaga agtcctggac tacctgcaac aagaaaatag ttacggtcat
     1021 cgggtgatgg cctcacaaca agccttgcag gatcgcatct taaaggaaat catcgaccgc
     1081 attccgcaac gagaagtttc tgcgccctac atcaaaaatg gctaccgcta tcggcatatt
     1141 tatgaaccag gctgtgaata tgctatctac cagcgtcaat cggcattcag tgaagagtgg
     1201 gatgagtggg aaacattgct cgatgccaat aagcgcgcag ctcatagtga gttttattcg
     1261 atgggcggaa tggcgattac gcccgataac accattatgg cgctggcaga agattttctt
     1321 tcccgacgcc agtacggcat tcgttttcgt aatctggaaa ctggtaactg gtacccggaa
     1381 ctgctggata acgttgaacc cagctttgtc tgggcaaatg actcctggac tttctactat
     1441 gttcgcaagc atccggtgac gctgctgcct tatcaggtct ggcgtcacgc catcggtacg
     1501 ccagcatcgc aagataaact gatctacgaa gaaaaagacg atacctatta cgtcagcctg
     1561 cataaaacga cctcgaagca ctatgtagtc attcatttgg ccagcgccac caccagtgaa
     1621 gttcgcctgc tggacgcgga aatggccgat gccgagccgt ttgtttttct gccgcgccgc
     1681 aaagatcacg aatacagcct tgatcactac cagcatcggt tttatctgcg ttccaaccgc
     1741 cacggcaaaa actttggctt ataccgtacc cgcctgcgtg atgagcaaca gtgggaagag
     1801 ttaattccgc cacgcgaaaa catcatgctg gaagggttta cgctgtttac cgactggctg
     1861 gtggttgaag agcgtcagcg cgggttaacc agtttgcgcc aaattaaccg caagacccgg
     1921 gaagtcattg gtattgcctt tgatgatcca gcctatgtga cctggattgc ctacaatcca
     1981 gaacctgaaa ccgcgcgatt gcgttatggt tattcttcca tgaccacacc agacactttg
     2041 tttgaactgg atatggatac cggtgagcgt cgtgtattaa aacaaacgga agttcctggt
     2101 tttgatgcgg cgaattaccg cagtgaacac ctgtggatag tcgcccgtga tggcgtcgaa
     2161 gttccggttt cgttggtcta ccatcgcaaa cattttcgca aaggacacaa cccgttgctg
     2221 gtgtatggct atggttctta cggcgcaagt attgatgccg atttcagttt tagccgcttg
     2281 agtttgttag atcgtggctt tgtctacgcc attgtccatg ttcgcggcgg tggtgagctg
     2341 gggcaacaat ggtacgaaga cggaaaattt ctgaagaaga aaaatacgtt taatgattat
     2401 cttgatgcct gcgatgcatt gttaaaactg ggctatggct ctccttcgct ttgttatgcg
     2461 atgggcggga gtgcgggggg catgttgatg ggcgttgcaa ttaatcaacg cccggaatta
     2521 ttccacggcg ttatcgccca ggtaccgttt gttgatgttg taacaacgat gcttgatgaa
     2581 tcaattcctc ttaccactgg tgagtttgaa gagtggggta acccgcagga tccgcaatat
     2641 tacgagtaca tgaaaagcta cagcccgtat gacaacgtca ccgcacaggc ttatccgcat
     2701 ttactggtaa cgaccggttt acacgattct caggtgcaat attgggaacc ggcaaaatgg
     2761 gtcgctaaat tgcgcgagct gaaaaccgat aaccatcttt tattgctctg taccgacatg
     2821 gactcaggcc atggcggtaa atctggccgc tttaaatcgt acgcaggcgt agcgatggaa
     2881 tatgcttttc tggtcgcgct ggcgcaggga acattacccg ctacgcctgc ggattaagta
     2941 ttttccagat aatgtttcag tgttaaacgc agctccgggc tcatgctgtc caggttatta
     3001 aatgaccagc gcagatagcc cggatcgcgt tcggcaacgt cggaaaccgc tttgccacgg
     3061 tatttgccaa aggtgaaggt cgtcatcaac gacggacgtc cggtgatatc ggccatctgt
     3121 tctgccgtcc agccggaggt gttcataata tcgataagca acgcggcggt gatataacag
     3181 tcatacaacg cgcggtgatg atgcaggccc ggcggcgtct gtacattgag cttccgtgtt
     3241 ttatataacg ccatattgct gtacttgatc cctggccaca aacgacgggc cagtttcata
     3301 gtgcaaatcc actcaccggg catctcaggc agtacgcggc ggtcaaagct ggcgttgtgc
     3361 gcgacatacc attcactacc gtaatagtgt gggatcacat cttcaatcca cggtttatcg
     3421 gcgaccatgg cttcggtgat gcgatgaatc gccatcgctt gtggactaat aggacgatcg
     3481 gggcgcacca ggtggctcat ggggttgacg atttttccgt caatgacatc aacagaggca
     3541 atctcaacga tccctccctg caaaccgcag gtttctgtat cgataatgcg caacatggca
     3601 tgctccaggc cgaaaaagcc tagcgtaatg gaatgatatc gccttgccaa ccctgtgatg
     3661 aacgctgacc gactaacaat aatgaaccac gatcggcgcg aacgatgaga cgaccatatt
     3721 catcccatag tgcactgtta ccacgggcat ttgccatcag cacggcaatc gagtactgat
     3781 gtgaaaaaaa ttgtaagcga cgggccgacg ccagcaggtc gggttctccc agacattgcc
     3841 ctgtagtaaa cagtgaacag gtcgggtcca tatccatacc ttgcggttgt tcatcgacca
     3901 ccgtgatggt tctggaacga cggcccagac atgcaccatg actctgatgg tagatcccag
     3961 gcgtttttcg ccacggtgcg aacaccgcaa ttccacgaat aaagcgatcg ttatattcaa
     4021 cgggaaggcc ggcaatgatg gtcattcgcc aggtcgttgc ggcataacaa agcgggtcta
     4081 acagtgagag atcgggtggg gcaggaaggg cacgtcgcga ataatcacac cccagtaaag
     4141 aaagcgatgg aaaaactaac agctgacatt gttgcctggc ggcggccctc acaaactcca
     4201 gatgatgagc tacctgttcg gtgagtgagg ttttacgagg ctcatattgc gctgctgcaa
     4261 ctttccagaa cgacataatg acttcctttt caaaatgact gcaaattatg ggtgcggttt
     4321 agaaaatact cataatttag caggcgttat gtaagaaagt gtaactctgt ataagtttta
     4381 tttaagtttg ggctcgtaag gtaaacgtga cagtttgacc gaagccaaac ggtgggcaat
     4441 aagcctctcg cgaaaccagc tgcgcaaatg ttcaggctgt tcacgttcaa ccgcttcagc
     4501 gatcaccggc atattgtagc gttctttaaa tgccaccccg gccgccgcca aatcgacatt
     4561 cactttatcc atttctgttt gatccagttt agccagattc ttcagcattc ttaatctcct
     4621 catcattcgc gggcaaaagt tactaagagt gagccaggat ggcaagccgg aattgatgat
     4681 cctcgactca gacattagac aaggttatgc tctgtaaaaa agttattaca aaaaggaata
     4741 gataatatgg cttcaactgc acgctccctt cgctacgcgc tggcaatcct gaccacttcg
     4801 ttggtgacac cttccgtctg ggcacatgcg catttaacgc atcagtatcc tgcggcaaac
     4861 gcgcaagtga cagctgcacc gcaggcaatc actttaaact tctcggaagg tgttgaaaca
     4921 ggattcagtg gtgcaaaaat cacggggcca aaaaacgaaa atattaaaac attaccggcg
     4981 aagcgaaatg agcaggacca aaagcaactg attgttccac tggccgattc gctgaaaccc
     5041 ggaacctaca ccgttgactg gcatgttgtt tcggtggacg ggcataaaac gaaaggacac
     5101 tacaccttta gcgtgaaata aatcatgctg gcgtttacct ggatcgcgct gcgatttatc
     5161 ca
//
LOCUS       DAJTNA010000233         5162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTNA010000233 DAJTNA010000000
VERSION     DAJTNA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.238"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..314)
                     /locus_tag="OO161_003770"
     CDS             complement(<1..314)
                     /locus_tag="OO161_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5464658.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGR"
     gene            1139..1321
                     /locus_tag="OO161_003771"
     CDS             1139..1321
                     /locus_tag="OO161_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464659.1"
                     /translation="MKWFIPEHVVEAFKKGELTRHQVVMNRNMARSRGYPEREKCFDD
                     ALKIIDELRKAEAEKE"
     gene            1468..2187
                     /locus_tag="OO161_003772"
     CDS             1468..2187
                     /locus_tag="OO161_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464660.1"
                     /translation="MIKNIKTIAEAKAKCLNQKDGKQKYVVVEQWLKMFKKWYSPSGN
                     EYTATLPAVEIYSYIAGFNEQGQECYATQKTLANIGMVSDRQVRNIINMFVDMGVLSV
                     EARHGQTSLYKALPFTDAHITAPREAKASVNAEKPQTEQVSTVKAVPEQLPPTEDKKT
                     LETTRSIQQEKTELPWGGVTICKPNGQPTEAALEWALAETQGDNIEAYRLISIVVSEF
                     TGREHIYTIPETEEDDFDIPF"
     gene            2199..2444
                     /locus_tag="OO161_003773"
     CDS             2199..2444
                     /locus_tag="OO161_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464661.1"
                     /translation="MIQYLVKNQVDRIQCNDTGKHTYETLAYLYKGKPTPLKYSDVLH
                     RAGCSESGLKFWLKQLSNFGVIEMKELSFSTFNLKKL"
     gene            complement(2649..2855)
                     /locus_tag="OO161_003774"
     CDS             complement(2649..2855)
                     /locus_tag="OO161_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HCR5464662.1"
                     /translation="MNNDRTQIYENIKQAISSASRNSQTMEMHLQMTKYVDHLKNVTA
                     KEFCEGVGLKASFGTEFSKMRNLT"
     regulatory      3064..3160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3195..3983
                     /gene="thiM"
                     /locus_tag="OO161_003775"
     CDS             3195..3983
                     /gene="thiM"
                     /locus_tag="OO161_003775"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5464663.1"
                     /translation="MQIDLLSPTQSARALHLFQQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFASIANALLINVGTLTQPRAKAMRAAVEQAHIAQTPWTLDPVA
                     VGALDYRRRFCRELLSLKPAAIRGNASEIIALAGASRGGRGVDTTDAAADALPAAQSL
                     AQESGAVVAVTGEVDYVTDGSRIVGICGGSLLMTKVVGTGCALSAVVAASCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFIAHFLDALWQLAPEVKA"
     gene            3980..4780
                     /gene="thiD"
                     /locus_tag="OO161_003776"
     CDS             3980..4780
                     /gene="thiD"
                     /locus_tag="OO161_003776"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5464664.1"
                     /translation="MKRINALTIAGTDPSGGAGVQADLKTFSALGAYGCSVMTALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVLSDVRIDTTKIGMLAEADIVEAVAERLQRYQIENV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARNEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTRDGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHKNWADTVQEAKSWLSAALAQADTLEVGHGIGPVHHFHAWW"
     gene            4846..>5162
                     /locus_tag="OO161_003777"
     CDS             4846..>5162
                     /locus_tag="OO161_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5464665.1"
                     /translation="MQLKIVSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAVQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGE"
BASE COUNT         1478 a         1092 c         1194 g         1398 t
ORIGIN      
        1 cgcccgtatt caacggcttc tttaaggagt tcattttcca tcgttttttt gccgagcagg
       61 cgctggagtt ctttaatctg cttcatggca gcagcaagtt cagaggcagg aacgacctgc
      121 tctcctgcgg ccacagcagt aagacttccc tcctggtatt gcttgcgcca gagaaataac
      181 tggctggctg ccacaccgtg ttgccgggca acaagggaga ccgtcattcc cggttcaaaa
      241 ctctgctgaa caatagcgat cttttcctgt gtagtacgcc gtctgcgttt ctccggtcct
      301 aagacatcaa tcatctgctc tccaatgact agtctaaaaa ctagtattaa gactatcact
      361 tatttaagtg atattggttg tctggagatt cagggggcca gtctatgatc ttattaatct
      421 tatctatctt agttaatctt cttacttatc ttacttactt tatctacttt attattaata
      481 atagcttcat aagctctctg tgtagctctc tgacgcaaga aacagagttc aggaagggaa
      541 tcaccttaga aaagtgttct ttgcgtcata atgtatttta tgaaggattc agaggtgttt
      601 cataacagag atgtatagag gatactttag gtaattaaaa ataaggggtc taaatgcttc
      661 tctaacaaca tcaagcaggg tagagaatgg aaaccgcttt gcctacatat ataaactcac
      721 tacagggctt tacagagcgt tttataaagt aactaatcta agaactctct gttttatatc
      781 tatcatcatc taacaaatac aaaataagcc acttaaataa gcctctggcg agctaaacag
      841 ccattacata agagaatgta ttgcttttat caaaacttcg ttagaacgcg ttttagagcg
      901 ttttagctgg taatgaagaa tagcactaca caggagagca cgaatcgcta aggaaaaatg
      961 taagtacatg aaaaagttct gattttttat gaaaagatat tgacaaatgt ttatttatat
     1021 gtaataatag gtactagagg gacagaaaaa cattcttctc taaataatca aaatcaatgc
     1081 actttgaagc cgtcaggctt gcaaggtgtt tttcttttaa ctgaataagg agttttttat
     1141 gaaatggttt ataccggaac acgtcgtaga ggcatttaaa aaaggtgagc taacacgaca
     1201 tcaagtagta atgaatcgta atatggcaag gagtcgaggt tatccagagc gtgaaaaatg
     1261 ctttgacgat gcactcaaga ttattgatga actgcgtaag gcagaagcag agaaagaata
     1321 ataacaggag aggagaaagc caaatgaata ttaattttga ctctgcaact tataacgcgt
     1381 tgttaaatcg cgcaaaggca gaagggaaat caattccaaa acttgttaat gaactactaa
     1441 ttacagtcct tacaaaggag gaactaaatg atcaaaaata tcaaaacaat tgccgaagcg
     1501 aaagccaaat gtctaaatca gaaagatggt aaacaaaaat atgtagttgt tgagcaatgg
     1561 ttgaagatgt ttaaaaaatg gtactcacca tctggtaatg aatacaccgc tacactgcca
     1621 gccgttgaaa tctatagcta tatagctgga ttcaatgaac aagggcaaga atgttacgct
     1681 actcaaaaaa cattagctaa tattggaatg gttagtgatc gtcaggtacg caatattata
     1741 aatatgtttg ttgatatggg agttctttct gtagaggcac gccacggtca aacatcactc
     1801 tataaagcat taccctttac agatgcacat attacagcac caagagaagc gaaagcctct
     1861 gtaaatgcag aaaagccaca gacagagcaa gtatctactg ttaaagctgt tcctgaacag
     1921 cttcccccta cggaagataa aaaaacacta gaaacaacac gctctattca gcaggaaaaa
     1981 acagagcttc cttggggcgg tgtcactatt tgcaaaccga atggacagcc aacagaagca
     2041 gcccttgagt gggctttagc tgaaacgcag ggcgataaca tagaggcata caggctaatt
     2101 tcaatagtgg tttctgaatt tacaggaaga gaacatatat acacgatacc tgagacagaa
     2161 gaggacgatt ttgacattcc attttaataa ggaggactat gatccaatat ttagtaaaaa
     2221 atcaagtaga ccgcattcag tgtaacgaca ccggaaaaca cacctacgaa acgcttgctt
     2281 acctctataa aggaaagcca acaccgctaa aatatagcga tgtgttacat cgagcaggtt
     2341 gctctgagtc aggtttaaaa ttctggctga aacagctatc aaatttcggt gttattgaaa
     2401 tgaaagagct gtctttttct acatttaacc tcaagaagtt atagaaaata gaattcatct
     2461 attacacgct ctaattctat ttctctgttt aatataaaag gaaaacatta ttatggtaat
     2521 agctatgtgg tggttgttgt tttttatgct cgtagaaaca tatgcaagta agtctggaaa
     2581 acaaaagtaa tatattgatg atttgttttt tataacttcg tggtgtcaag tccggcagct
     2641 ttgagtcgct aggtaagatt tctcattttg ctgaactctg taccaaagct ggctttaagt
     2701 cctacacctt cgcaaaactc ttttgccgtg acgtttttca gatggtcaac atatttggtc
     2761 atctggaggt gcatttccat cgtttggcta tttcgtgatg cgctgctaat ggcttgctta
     2821 atgttctcgt agatctgggt tctgtcattg tttatcccac ctatcaaatt tagaaaaata
     2881 atattaatta gctaattaat ataatcaatg gttttcacca tctgaatgat agtcgttaat
     2941 tgtttctttt tcattccttc tatggggtac aaaatggggt acacagagaa gaggcaatta
     3001 atggaggcga gcttgcatct gcaagccact tcccctaagc tggcacaaaa tatagagatt
     3061 caaccaaacg actcggggtg cccttctttg tgaaggctga gaaatacccg tatcacctga
     3121 tctggataat gccagcgtag ggaagtcacg gaccgccagg tcattgcttc ttcacgttat
     3181 ggcaggagca aactatgcaa atcgacctgc taagtccgac gcaatctgcg cgcgctttac
     3241 acctgtttca acaacattcc ccccttgtac actgcatgac caatgatgtg gtgcaaacgt
     3301 tcaccgccaa tacattgctg gcgttgggcg catcgccagc catggtgatt gaaacggaag
     3361 aggccagtca atttgcgtcc attgccaatg ccttgttgat taacgtcggc acgttgacac
     3421 agccacgtgc gaaagcgatg cgtgcagccg tcgaacaggc gcatatcgca caaacaccct
     3481 ggacgcttga tcccgttgcg gttggcgcgc tcgactatcg ccgccgtttt tgtcgtgaac
     3541 ttttatcgtt aaaacctgct gcaatacgag gaaatgcgtc ggaaatcatt gcattagcgg
     3601 gagcttcacg aggtggacgt ggtgtggata ctactgacgc cgcagcagac gcgttgcctg
     3661 ctgcacaatc cctggcgcaa gaatctggtg cagtagtcgc ggtcacaggc gaggtggatt
     3721 atgttaccga tggcagccgt attgttggca tttgcggtgg cagtctgcta atgaccaaag
     3781 tggtcggaac tggttgcgca ttatcggcag tggtcgctgc cagttgtgca ttaccgggcg
     3841 atacactgga aaacgtcgca tctgcctgtc actggatgaa acaagccgga gaacgcgctg
     3901 ttgccagaag tgaagggccg ggcagtttta ttgcacattt tcttgatgcg ctctggcagc
     3961 tggcaccgga ggtgaaggca tgaaacggat taacgctctg acgattgcag gcaccgaccc
     4021 gagcggtggt gcgggagttc aggccgatct aaaaaccttc tccgcgcttg gtgcatacgg
     4081 ttgttcggtg atgaccgcgc tggtggcgca aaatacccgc ggcgtacagt cggtgtatcg
     4141 tatagaacct gattttgttg ccgcccaact cgattccgtg ctcagcgatg ttcgtattga
     4201 taccactaaa atcggcatgc tggcagaagc tgatattgtt gaagccgtgg cggaacggct
     4261 gcaacgttat cagatcgaaa acgtagtact ggatactgtc atgctggcaa aaagtggcga
     4321 tccgctactt tcaccttcgg cggttgccac tcttcgcagt cgattattgc cgcaagtttc
     4381 attgataact ccaaacctgc cggaagcagc ggccttgctt gatgctccac acgctcgtaa
     4441 tgaacaggaa atgctggagc aggggcgcgc cttattggcg atggggtgcg gtgcggtact
     4501 gatgaaaggt gggcatctgg atgatgaaca aagcccggac tggctgttta cccgtgacgg
     4561 cgaacaacgt tttaccgcgc cgcgcattat gactaaaaac acgcatggca caggttgtac
     4621 gctctcggcg gcgttggcgg cgctacgtcc gcggcataaa aactgggccg atactgttca
     4681 ggaagccaaa agctggctgt cagcggcgtt agctcaggcc gacacgctgg aagtcggtca
     4741 tgggattggt ccggttcacc acttccacgc ctggtggtga ccgccgtact atagctgctt
     4801 tggttattgt cagccttttt gcgctgaaat tcagggatga aaccaatgca gctaaaaatc
     4861 gtcagtcgca aaaaattcac cgtcttgtta tgcgctctgg ggctaatttc catcgttgcg
     4921 atttatcccc gccagacggt gaattttttc tattccacgg cagttcagat taaagactac
     4981 attcactttt atggttatcg tccggtcaaa tcttttgcta ttcgcattcc tgccagttac
     5041 accattcatg gcatagatgt ttcacgctgg caggagcgga tagactggca gcgtgtggca
     5101 aaaatgcgcg acaacggtat ccgcctacag tttgctttta ttaaagcgac ggaaggcgag
     5161 aa
//
LOCUS       DAJTNA010000234         5130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTNA010000234 DAJTNA010000000
VERSION     DAJTNA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.239"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..564
                     /locus_tag="OO161_003778"
     CDS             <1..564
                     /locus_tag="OO161_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5464666.1"
                     /translation="KAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQPDTQTAEQDFL
                     TRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVRPKEPNWTAWA
                     NEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELSLRLSPSVSTH
                     TEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            561..1055
                     /locus_tag="OO161_003779"
     CDS             561..1055
                     /locus_tag="OO161_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5464667.1"
                     /translation="MSMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPGPQSVRETRPVRNFYVGTNGPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAVSGDEWYLSGN
                     YVGA"
     gene            1055..1708
                     /locus_tag="OO161_003780"
     CDS             1055..1708
                     /locus_tag="OO161_003780"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR5464668.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPSWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            1705..2031
                     /locus_tag="OO161_003781"
     CDS             1705..2031
                     /locus_tag="OO161_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5464669.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENAKLRAAHWLH
                     ERGLKV"
     gene            2028..2417
                     /locus_tag="OO161_003782"
     CDS             2028..2417
                     /locus_tag="OO161_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5464670.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLNNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKPAGAAA"
     gene            2437..3234
                     /locus_tag="OO161_003783"
     CDS             2437..3234
                     /locus_tag="OO161_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR5464671.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQTADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            3242..4230
                     /locus_tag="OO161_003784"
                     /pseudo
     CDS             3242..4230
                     /locus_tag="OO161_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            4244..4996
                     /locus_tag="OO161_003785"
     CDS             4244..4996
                     /locus_tag="OO161_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5464672.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTR"
BASE COUNT         1262 a         1211 c         1480 g         1177 t
ORIGIN      
        1 aaagcgggtt ttcacccgtc agaatctggc ggggatccgt cagtaaaatc aaaacatgat
       61 ccgtcagata aaaaaccttc tcgtccggac gcttcgcaac cggacacgca gacggctgaa
      121 caggattttt taactcgcca tcctgatgcg gttgtattca gccctaaaaa gcgccagtgg
      181 gggacgcagg atgatttgac ctgcgcacag tggctctgga aaaaaatcat cgccctgtac
      241 gagcaggctg ccgaatgtga cggcgaggtg gttcgtccca aagaaccgaa ctggacagcc
      301 tgggcaaacg aaattcgcct gatgtgtgta caggatggcc gtactcacaa acaaatctgc
      361 gagatgtaca gccgcgtcag ccgcgatccg ttctggtgcc gtaacgtgct cagcccgtcg
      421 aagttgcggg aaaaatggga tgagctttcc ctgcgcttat cgccgtccgt cagcacgcac
      481 acagaaaaac gtgaagaccc gtacttcaaa gccagttacg acaacgtgga ctacagccag
      541 atcccggcag gattcagggg gtgagcatga gtcttttgaa tgacgttcag aaattcattg
      601 aagcccatcc ggggtgtact tccggagaca ttgcggatgc ttttgcaggt tactcacggc
      661 agcgcgttct gcagtctgca agcaagttac gtcagagtgg gcgtgtggct caccgttgtg
      721 aaggagatac acgcagacat ttcccgcgcc tgactgagag agcgcaggaa ccgggaccac
      781 aatcagttcg agaaaccaga cctgtgcgca atttctatgt cggcactaac ggtccccggg
      841 tgattttgtg cctgacccgc caggctgaag aactggagtc caggggctta taccgtcgtg
      901 ctgcaacggt gtggatggcg gcattccgtg aaagccactc ccagccagaa cgaaacaatt
      961 ttctggcgcg tcgtgagcgg tgcttacgga aaagcagcaa gcgcgctgta tcgggtgatg
     1021 agtggtatct gtcagggaat tacgtggggg cttaatgagt aataaatatt gccaggcgct
     1081 ggtggagttg cgaaacaaac cagcccatga actgaaggaa gtgggcgatc agtggcgcac
     1141 accggacaac attttctggg gaattaacac cctgtttggc ccgtttgttc tggatctgtt
     1201 tactgacggt gataacgcca aatgtgccgc gtattacacg gcggaagaca acgcgctggc
     1261 gcatgactgg tcagaacgtc ttgcggagct taaaggtgct gcctttggaa atcctccata
     1321 cagccgcgcc agtcagcatg aggggcaata catcaccggc atgcgttaca tcatgaaaca
     1381 tgccagtgcc atgcgtgata agggcgggcg ctatgttttc ctgatcaaag ctgccaccag
     1441 cgaagtgtgg tggccggaag atgcggatca tattgctttt attcgcgggc gtattggttt
     1501 tgaactgcct tcctggttta tcccgaaaga cgagaagcag gtgccaaccg gtgctttctt
     1561 cgctggtgct attgccgttt tcgacaagac ctggaaggga ccggcaatca gctacatcgg
     1621 gcgcgatgaa cttgaggcat gtggtgaggc ctttctggcg caagttcgcc agcaggcaga
     1681 aaaacttgtc agggagatgg cggcatgacg acgttaactc aatgccagca gcaggtgctg
     1741 gatatgctga tttcttacca gaaagaacgt ggcttcccgc caaccaatca ggaggtggca
     1801 accatgctgg gataccgttc agtgaatgca gcggtggagc atcttagagc actggagaaa
     1861 aaaggcgtca tcacgataaa gcgtggtgtg gcccgtggta tcactcttca taccgcggtg
     1921 aaggacgacg acagcgaggc ggtcgggatt atccgctcac tgcttgccgg tgaggaaaac
     1981 gccaagctgc gtgcagccca ctggttacat gagaggggcc tgaaagtatg aagctaatac
     2041 tgccttttcc gcccagcgtg aacacgtact ggcgacaccc caacaaaggg gcgtttgctg
     2101 gtaagagcct gataagcgca gcggggcgca aattccagag cgcggcgtgt gcagcaatag
     2161 ttgagcagtt acgtcgtctg ccaaaaccaa cgtcggcacc tgcttcagtg gagatcgtgt
     2221 tgtttcctcc ggataaccgg atccgcgatc tgaacaacta taacaaggcg ctgtttgacg
     2281 ccctgaccca cgcgggtgtg tgggaagacg acagtcaggt gaaaagaatg ctggtggagt
     2341 ggggaccggt tatcccggaa gggaaggtcg agatcactat cagtaagtac gagaaaccgg
     2401 cgggtgcagc agcctgatta agaggagaaa cgaagtatga ataatctgat ggttattgat
     2461 ggaattgaag ttcgtcgtga tgcttatggg cgttacagcc tgaacgatct gcacagggct
     2521 gctggttctc tggataagca taagcctgca ttctggctcc gcaatgagca aactgaacgt
     2581 ttaataagcg agttgcagat ttgcaactcg gtcaatatag aaccagttaa cgttattcgt
     2641 ggcggaaata accaagggac gtatgtctgc aaagaactgg tgtatgccta tgcaatgtgg
     2701 atcagcccgt cattccatct gaaggtgatc cgtactttcg acatggtaac cagtgcaccg
     2761 gaaaaattat ccggacagac tgctgacaag atgcaggctg gtgtgattct gctggacttt
     2821 atgcgccggg aattaaacct gtctaactct tcagtgcttg gagcctgtca gaaactccag
     2881 gaggctgttg gcttaccgaa tctggcaccg cgctatgcca ttgatgctcc tgctgacgcg
     2941 cctgatggct caagccgccc cacgctgtca ctgagtgcac tgctgaagca gtatggtatc
     3001 cgccttacgg ctaatcaggc atatcaccag atggcgaagc tggggatcgt cgaacagcgc
     3061 gaacgataca gccgtacagc gattaacaac atcaaaaaat tctggtcgct gacagcgaaa
     3121 ggctgcatgt tcggcaagaa tatcaccagt cccgcaaatc cgcgtgagac gcagccgcat
     3181 ttcttcgaat cccgattccc tgagctgtta aagctgctcg ataccgttca ttgaggtgac
     3241 cgtgagagca ctactgcccc ctgaaattgc cccgcgtatg gggatcgtat tgttcagacc
     3301 aagttcagag ctgatgcccc tgtttatgca ggggcgtgtc ctgctggagc ctgagccgga
     3361 acgttattca tctttcgcca gtggtgctgt tccggcggca tcacaaccgc tggcggatga
     3421 tcctgacgtt cgggccgtgt tccgcaatga ggcagtgatc cgtcgtgctg gtggcgtgga
     3481 atgtcttgaa agctggttac ttcgtgaaaa aggctgccag tggcctcatt ccgactggca
     3541 cagcgagaac atgacaacaa tgcgacacgc gccggcgcaa tccgtctgtg ctggcactgc
     3601 gataaccagc tgcgcgatca gttcacggaa cggctggaat caatggcaac ggataactgt
     3661 gcccgctggg tgttgtctgt tgtgcgtcgg gatctcggtt ttgatgatag tcacgttgtg
     3721 acaatgccgg aactgtgctg gtggctgatt cgtaatgacc tggcggatgc cttaccggaa
     3781 agtgcagccc gtaaggcact gagattaccg aagcctgttg tgccgtctgt cacccgggaa
     3841 agtgaccttg tgccttcggt tcctgccacc agcatcatcc aggataaggt gaaaaaggtg
     3901 ctggcgctga aagtggatcc ggagtcgccg gagtctttta tgttacgccc aaaacgtcgc
     3961 cgctgggtta atgaaaagta cacacgctgg gttaagacac agccgtgtgc atgttgtgga
     4021 aagcctgctg atgatcccct ccacctgata ggccacggtc aggggggaat gggtacaaaa
     4081 gcgcatgacc tctttgtgtt gcctttgtgc agaaagcatc acgacgagct gcatgcggat
     4141 accgtggcat ttgaagagaa gtatggttcc cagctggagc tgatatttcg ttttatcgat
     4201 cgtgcgctgg caataggcgt actggcgtaa gtggagaacg agcatgaacc ttgaagcctt
     4261 accgaaatat tactccccga aatctccaaa actgagcgat gacgcaccgg cgacaggctc
     4321 tggtggttta acgattacgg atgtgatggc tgcgcagggg atggtgcagt cgaaagcacc
     4381 gcttgggttt gccttattcc tggcaaaagt tggtgttcag gatcctcagt ttgcgattga
     4441 aggtctgctc aattacgcga tggcactgga taacccgaca ttgaataaat tgagtgaaga
     4501 aacccggcta cagatcattc cttaccttgt gaattttgcc tttgctgatt attctaggtc
     4561 tgcggcaagt aaggctcgct gtgagcattg tgctggtact ggatttcata atgtattgcg
     4621 cgaggtggtg aaacactcca gaagcgggga atctgttatc aaggaagagt gggtgaagga
     4681 actatgtcag cattgtcatg gtaagggaga agtcagcaca gcgtgcagag ggtgtaaggg
     4741 taaaggtatt gtcctggatg aaaaaaggac ccggcttcat ggcacgcctg tttataagat
     4801 ttgtgggcgt tgcaatggaa accggtttag ccgtttacca actacactgg cacgacgtca
     4861 tgtccagaag ctggttccag acctgactga ttatcagtgg tacaaaggat atgcagacgt
     4921 aattgataaa ctagtaacaa agtgctggca ggaagaagca tatgctgaaa cacaattgag
     4981 aaaggtgacg agataagtga ttttcgccga agatggcgac atgaagcttg catttttcaa
     5041 aaaacatgga taagattttc tcaacgatgg gctttgtatg tctgccgttg ataattttca
     5101 cgaacccgct gttgagcggg tttttattta
//
LOCUS       DAJTNA010000235         5121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTNA010000235 DAJTNA010000000
VERSION     DAJTNA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.240"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            28..1956
                     /locus_tag="OO161_003786"
     CDS             28..1956
                     /locus_tag="OO161_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5464673.1"
                     /translation="MISDAQKAANAAGAIATGLLSLIIPVPLTTVQWANKHYYLPKES
                     SYTPGRWETLPFQVGIMNCMGNDLIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVPALLELAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSSTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDASPFGLKWEKNKPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTTAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVDEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            1940..2146
                     /locus_tag="OO161_003787"
     CDS             1940..2146
                     /locus_tag="OO161_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5464674.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2143..3735
                     /locus_tag="OO161_003788"
     CDS             2143..3735
                     /locus_tag="OO161_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5464675.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYTGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNSVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3725..>5121
                     /locus_tag="OO161_003789"
     CDS             3725..>5121
                     /locus_tag="OO161_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5464676.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGILLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVRQDMQQRIDAAHRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAVQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTAL"
BASE COUNT         1141 a         1231 c         1629 g         1120 t
ORIGIN      
        1 ccggtgaaaa cgtgggcggg tggatcgatg atttcagacg cgcagaaggc agctaatgca
       61 gccggtgcga tagctacagg gcttttatct ctcattattc ctgttccact gacgacagtt
      121 cagtgggcca ataaacatta ttaccttcct aaagagtcgt cttatacccc ggggcggtgg
      181 gaaacactgc cgtttcaggt tggcatcatg aactgtatgg gcaacgatct gattcgtacg
      241 gttaacctga ttaaatctgc ccgtgttggt tatacaaaga tgttgctggg agtggaggct
      301 tattttattg agcataaatc acgcaacagc cttctttttc agcccacgga ctcagctgct
      361 gaagatttta tgaaatctca tgttgagcca acgataaggg atgttcctgc attgctggag
      421 ctggctccat ggttcggaag aaaacaccgc gataatacgc tcaccctgaa gcgtttttcc
      481 tccggtgtgg ggttctggtg tctgggtggt gcggcagcaa aaaactaccg tgaaaaatcc
      541 gtggatgtgg tctgttatga cgagctttcc tcgttcgaac cggatgttga aaaagagggt
      601 tcgtcaaccc tgctggggga taaacgtatt gagggctctg tatggccaaa atccattcgc
      661 ggctcgacgc ctaaaatcaa aggctcctgc cagatcgaaa aagccgctaa cgagtcggca
      721 cacttcatgc gtttttatgt gccctgtccg cactgtgggg aggagcagta tctgaaattt
      781 ggcgatgatg cctcgccttt cggtcttaag tgggagaaga ataagccaga aagtgttttc
      841 tacctttgtg agcatcatgg ctgtgtgatc catcagtctg agcttgacca gagtaacggg
      901 cggtggatct gtgaaaacac gggcatgtgg acccgtgacg gcctgatgtt tttcagcgcc
      961 cggggtgatg aaattccgcc gccgcgctcc atcactttcc atatctggac ggcgtacagt
     1021 ccgttcacca cctgggtaca gattgtctat gactggctgg atgcactgaa agatcccaac
     1081 ggcctgaaaa cctttgtgaa caccacgctg ggcgagacct gggaagaggc cgtgggcgaa
     1141 aaactcgatc accaggtact gatggataag gttgtgcgtt acacgacggc ggtgcctgcc
     1201 cgggtggttt atctgacggc gggcattgac tcgcagcgaa accgttttga gatgtatgtc
     1261 tggggatggg ctccgggaga ggaagccttt ctggtggata aaatcatcat tatggggcgt
     1321 cccgatgagg aagagacgct attacgtgtg gatgcggcga tcaacaaaaa ataccgccat
     1381 gcagacggaa ccgaaatgac tatttcccgt gtctgctggg acatcggggg gattgatggc
     1441 gaaatcgttt atcagaggtc aaaaaaacac ggtgttttcc gggtgctgcc ggtaaaaggc
     1501 gcatctgtct atggcaagcc ggtgatcacc atgccaaaaa cccgcaatca gcggggcgtg
     1561 tatctgtgtg aagtggggac ggacaccgca aaagaaattc tctatgcccg tatgaaagcc
     1621 gatcccacgc ctgtggatga agccacgtcg tatgccatcc gttttcctga tgatccggag
     1681 attttttcgc agacagaggc gcagcaactg gtggcggaag agcttgtgga gaagtgggaa
     1741 aaaggaaaga tgcgtctgct gtgggataac aaaaagcggc gtaacgaagc gctggactgc
     1801 ctggtgtatg cctacgcggc attacgtgtg tccgtgcaac gctggcagct tgatctggct
     1861 gtactggcaa aatcccggga agaagagacg acccggccaa cccttaaaga actggcagcg
     1921 aagctgtccg gaggagtgaa tggttacagt cgctgaactg caggcgctgc gtcaggcgcg
     1981 ccttgattta ttaaccggta aacgggtggt gtctgtccag aaagatggtc gcagaattga
     2041 atatacggca gcttctctgg atgagcttaa ccgggcgatc aatgatgcgg agtcggtact
     2101 ggggacaacc cgccgtcgcc gtcgtccgct gggagtgagg ttatgaaacg aacgcctgtc
     2161 ctgattgatg tgaacggcgt tccgcttcgg gagagcctca gctacaccgg tggcggtgca
     2221 ggatttggcg ggcaaatggc agagtggttg ccaccctcgc agagtgccga tgcggccctg
     2281 ctgcccgcgt tgcgtctggg gaatgcccgt gcagatgatc tggtgcgcaa taacggaata
     2341 gcggccaatg cggtggcact gcataaggat cacattgtcg ggcatatgtt tctgattagc
     2401 taccgtccga actggcgctg gctggggatg cgggagaccg cggcaaaaag ttttgtcgat
     2461 gaggtggagg cggcctggtc agaatacgca gaagggatgt ttggtgagat cgacgtggaa
     2521 gggaaacgca cgtttacgga atttatccgt gaaggtgtgg gcgttcatgc gtttaacggc
     2581 gaaatctttg tgcagccggt ctgggatacg gagagtacgc aactgtttcg tacgcgtttt
     2641 aaagccgtga gtccgaaacg ggtggacacg ccaggacacg gtatggggaa ccgttttctg
     2701 cgggctggtg tggaggttga tcgatatggc cgtgccgttg cgtaccatat ctgtgaggat
     2761 gattttcctc gctccgggag tggacgatgg gaacggatcc cgcgtgaact acccaccggg
     2821 cgtccggcca tgctgcatat tttcgagccg gtggaggacg ggcagacccg tggagccaat
     2881 cagttttaca gcgttatgga acggctgaag atgctggatt ccctgcaggc aacacagctt
     2941 cagtcggcca tagtgaaggc gatgtatgca gcgacgattg aaagtgacct tgataccgaa
     3001 aaggcctttg aatatatcgc cggtgcgccg caggggcaga aggataatcc gcttattaat
     3061 attctggata agttctccac ctggtatgac acgaatagcg tgacgctggg cggtgtcaaa
     3121 attccgcacc ttttccccgg tgatgatctg aaacttcaga ccgcgcagga ttcagacaat
     3181 ggattttcgg cgcttgaaca ggcgctgctg cggtatatcg ccgccggtct tggcgtttcc
     3241 tacgaacagt tgtcccgtga ttactcgaag gtcagttatt caagtgcccg cgcatccgcc
     3301 aatgagtcgt ggcgctattt tatggggcgg cgaaaattta ttgcgtcccg gctggccacg
     3361 cagatgtttt cctgctggct ggaagaggca cttcttcggg ggattattcg tccgccacgg
     3421 gcacgttttg atttttatca ggcgcgatca gcctggtcac gggctgagtg gattggagcc
     3481 ggaagaatgg ccattgacgg gctcaaggag gttcaggaat cagtgatgcg cattgaggcc
     3541 ggactgagca cgtatgagaa agagctggcg ctgatgggcg aggattatca ggacattttc
     3601 cgccagcagg tcagggaatc tgcagagcgg gaaaaagccg gactctcacg tccggtgtgg
     3661 atagcgcagg cgtatcagca gcagatagcg gagagtcgca ggccggaaga ggagacaaca
     3721 ccacgtgaga cgtaatcttt cacacattat tgccgcagca ttcaatgaac cgctgcttct
     3781 ggagcccgcc tatgcgcggg ttttcttttg cgcgctcggg cgcgagatgg gcgcagcaag
     3841 tctttcggta ccacaacagc aggtacagct tgatgctccc ggaatgctgg ctgaaacgga
     3901 cgagtacatg gccggaggta aacgaccggc ccgtgtttac cgggtggtga acggtattgc
     3961 ggtactgccg gtgaccggca cgctggtgca ccggctgggg gggatgcggc cattttccgg
     4021 aatgactggc tatgacggca ttgtcgcctg tcttcagcag gcaatggcag atagccaggt
     4081 gcggggcata ctgctggaca ttgacagtcc gggtgggcag gccgccggcg cgtttgactg
     4141 cgctgacatg atttaccgcc tccgtcagca gaagccggtc tgggcactgt gcaatgacac
     4201 ggcctgttct gcagccatgc tgctggcgtc ggcctgctcc cgacggctgg ttacccagac
     4261 atcccgtatc ggctccattg gcgtgatgat gagccatgtc agctatgccg gtcatctggc
     4321 gcaggccggt gtggatatca cgctgattta tgccggggcg cacaaggtgg atggcaatca
     4381 gtttgaagcg ttgccggcag aggttcgcca ggatatgcag cagcggattg atgcggcgca
     4441 ccggatgttt gccgaaaaag tggcgatgta taccgggttg tctgtggatg cggtcacggg
     4501 aacagaggcc gccgtttttg aaggtcagtc cggcattgag gccgggctgg cggatgaatt
     4561 aatcaatgcg tcggatgcca tcagtgtgat ggccacggcg ctgaacagta atgtcagagg
     4621 aggcactatg ccgcaattaa ctgcaacgga agccgccgtg caggagaacc agcgagtgat
     4681 ggggatcctg acatgccagg aagcgaaagg acgtgaacag cttgccacga tgctggcagg
     4741 gcaacagggc atgagcgttg aacaggcccg ggcgattctg gccgcggcgg caccgcagca
     4801 gccggtggca tccgcgcaga gtgaagccga tcgcattatg gcgtgtgaag aagcgaacgg
     4861 tcgtgaacaa ctggcagcaa cgctggcggc gatgccggag atgacggtgg aaaaagcccg
     4921 cccgatcctg gctgcttcac cgcaggcgaa tgccggacca tcactccgtg atcagatcat
     4981 ggcactggat gaggcaaaag gggctgaggc gcaggctgaa cagctggctg cctgcccggg
     5041 aatgaccgtg gagagcgccc gggctgtgct ggctgcggga tcaggtaagg cagaaccggt
     5101 ctctgcatcc acaaccgccc t
//
LOCUS       DAJTNA010000236         5114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTNA010000236 DAJTNA010000000
VERSION     DAJTNA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.241"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            53..273
                     /locus_tag="OO161_003790"
                     /pseudo
     CDS             53..273
                     /locus_tag="OO161_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            270..1094
                     /locus_tag="OO161_003791"
     CDS             270..1094
                     /locus_tag="OO161_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5464677.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPRGSVGDSPRTSHSFEPVKEPVNEPIAVGASVDESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRGGL"
     gene            1091..1579
                     /locus_tag="OO161_003792"
     CDS             1091..1579
                     /locus_tag="OO161_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5464678.1"
                     /translation="MSLLNEVQKFIEAHPGCTSGDIADAFTGYSRQCVLQSASKLRQS
                     GRVAHRCEGDTRRHFPRLTERAQEAEPQPVRETRPVRNFYVGTNDPREILCLTRQAEE
                     LESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSGDEWYLSGNYV
                     GA"
     gene            1579..2232
                     /locus_tag="OO161_003793"
     CDS             1579..2232
                     /locus_tag="OO161_003793"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR5464679.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYIIG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            2229..2618
                     /locus_tag="OO161_003794"
                     /pseudo
     CDS             2229..2618
                     /locus_tag="OO161_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            2638..3447
                     /locus_tag="OO161_003795"
     CDS             2638..3447
                     /locus_tag="OO161_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR5464680.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     ISSQQTVELVHELTNTQNLGVDPVSMIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLFNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITCPANPRETQPHFFESRFPELLKLLDTVH"
     gene            3455..4444
                     /locus_tag="OO161_003796"
     CDS             3455..4444
                     /locus_tag="OO161_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5464681.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPDVRAVFRNEEVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPTVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            4462..4842
                     /locus_tag="OO161_003797"
     CDS             4462..4842
                     /locus_tag="OO161_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCR5464682.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
BASE COUNT         1257 a         1177 c         1477 g         1203 t
ORIGIN      
        1 tggcctgcct cataaatttg tcgctgaacc cttcagaggt aagggggcgc aaatgatccg
       61 gaatattttc aaacggttta ccaatcagac tttccgttgt cctcgtccgg gtcagtggta
      121 caccacgcct gcagggcatg ttctacgtgt tagcctggtc gtgaatgtca gaaggtgatt
      181 tgtgaaccgc tgggccgtaa ttaccgcatc agtatgccgc ttatagcctt tcgctccgga
      241 aaaaacatga agcatctcgg aggtgctgca tgagtatgga gctgatggtt aaagcgatga
      301 aaattcgagt gggaaatcca ttgcgaaaac tggttctgat taagctggct gataatgcca
      361 gcgatcaggg tgagtgctgg cccagctacc agcatattgc tgaccagtgc gagattagca
      421 aacgttctgt gatgaatcat attgcggccc tttgtgagtc cgggctggta aaaaaagtca
      481 cccggaaagg tgaaaaaggt aactcaagta atatctatct ccttcatctt gatggtgcag
      541 gagattcact agggggtagt gcaaataatt cactatctgg tgcagcaaat tcaccaggta
      601 gtgcaggagt tgcaccaagg ggtagtgtag gagattcacc cagaaccagt cactcttttg
      661 aaccagtcaa agaaccagtc aatgaaccaa tagctgttgg tgcatcagtt gatgagtccg
      721 tgcgagttcg ttcaaaccga ccggaatact ctccggagtt tgagcaggca tggctggcat
      781 atcccaaacg tgctggtggc aattcaaaat ctgcagcctt caaagcctgg aaagcccgtt
      841 tgaacgaggg ggtaaacccc gaaaccatgc tggaaggtgt gaaacgctac gcgggctggg
      901 tatctgcgat gggcaatagc ggcacacaat ttgtgaaaca ggctgtcacg ttctttggtc
      961 cagatcgtca tttcgaagaa tcctgggaag ttcctgcggt atctgcagcc agacgcgagg
     1021 acccgtactt caaagccagt tacgacaacg tggactacag ccagatcccg gcaggattca
     1081 gggggggatt atgagtcttt tgaatgaagt tcagaaattc attgaagccc atccggggtg
     1141 tacttccgga gacattgcgg atgcttttac aggttactca cggcagtgcg ttctgcagtc
     1201 agcaagcaaa ttacgtcaga gtggtcgtgt ggctcaccgt tgtgaagggg atacacgcag
     1261 acatttcccg cgcctgactg agagagcgca ggaggcggaa ccgcaaccag ttcgtgaaac
     1321 cagacctgtg cgcaatttct atgtcggcac taacgacccg cgggagattt tgtgcctgac
     1381 ccgccaggcg gaagaactgg agtccagggg cttataccgt cgtgctgcaa cggtgtggat
     1441 ggcggcattc cgtgaaagcc actcccagcc agaacgaaac aattttctgg cgcgtcgtga
     1501 gcagtgttta cggaaaagca gtaagcgcgc tgtatcgggt gatgagtggt atctgtcagg
     1561 gaattacgtg ggggcttaat gagtaataaa tattgccagg cgctggtgga actgcggaac
     1621 aaaccagccc atgaactgaa ggaagtgggc gatcagtggc gcacgccgga caacattttc
     1681 tggggaatta acaccctgtt tggtccgttt gttctggatc tgttcactga cggtgataac
     1741 gccaaatgtg ccgcgtatta cacggcggaa gacaacgcgc tggcgcatga ctggtcagaa
     1801 cgtcttgcgg agcttaaagg tgctgccttt ggcaatcccc cgtacagccg cgccagtcag
     1861 catgaggggc aatacatcat cggcatgcgt tacatcatga agcatgccag tgccatgcgt
     1921 gataaaggcg ggcgctatgt tttcctgatc aaagctgcca ccagcgaagt gtggtggccg
     1981 gaagatgcag atcatattgc ttttattcgc gggcgtattg gttttgaact gcctgcctgg
     2041 tttatcccga aagacgaaaa gcaggtgcca acaggtgctt tcttcgctgg tgctattgct
     2101 gtttttgaca agacctggaa gggaccggca atcagctaca tcgggcgcga tgaacttgag
     2161 gcatgtggtg aggcatttct ggcgcaggtt cgccagcagg cggaaaaact ggtcagggag
     2221 atggtggcat gaagctaata ctgccttttc cgcccagcgt gaacacgtac tggcgacacc
     2281 ccaacaaagg ggcgtttgct ggttagagcc tgataagcgc ggcggggcga aaattccaga
     2341 gcgcggcgtg cgcagcaata gttgagcagt tacgtcgtct gccgaaacca acgtcggcac
     2401 ctgcttcagt ggagatcgtg ttgtttcctc cggataaccg gatccgcgat ctggacaact
     2461 ataacaaggc gctgtttgac gccctgaccc acgcgggtgt gtgggaagac gacagtcagg
     2521 tgaaaagaat gctggtggag tggggaccgg ttatcccgaa agggaaggtc gagatcacta
     2581 tcagtaagta cgagaaaacg gcgggtgcag ccgcctgatc aagaggagaa acgaagtatg
     2641 aataatctga tggtcattga tggtattgaa gttcgtcgtg atgcttatgg gcgttacagc
     2701 ctgaacgatc tacatcgcgc agcagtagca tctggtgcaa atgccagaac caaggagccg
     2761 ggaaagttta tttccagcca acaaactgtt gagcttgttc atgaattgac caacacccag
     2821 aatttgggtg ttgacccggt gagtatgatt catgggggaa atgaacgggg aacgtatgtc
     2881 tgcaaggaac tggtgtatgc ctatgcaatg tggatcagcc cgtcattcca tctgaaggtg
     2941 atccgtactt tcgacatggt aaccagcgca ccggaaaaat tatccggaca ggctgctgac
     3001 aagatgcagg ctggtgtgat tctgctggac tttatgcgcc gggaattaaa cctgtttaac
     3061 tcttcagtgc ttggtgcctg tcagaaactc caggaggctg ttggcttacc gaatctggca
     3121 ccgcgctatg ccattgatgc tcctgctgac gcgcctgatg gctcaagtcg ccccacgctg
     3181 tcgctgagtg cactgctgaa acagtatggt atccgcctta cggctaatca ggcatatcac
     3241 cagatggtga agctggggat cgtcgagcag cgcgaacgat acagccgtac cgcgattaac
     3301 aacatcaaaa aattctggtc gctgacagcg aaaggctgca tgttcggcaa gaacatcacc
     3361 tgtcctgcaa atccgcgcga gacgcagccg catttcttcg aatcccgatt ccctgagctg
     3421 ttaaagctgc tcgataccgt tcattgaggt gaccgtgaga gcactactga cccctgaaat
     3481 tgccccgcgt atggggatcg tattgttcag accaggttca gagctgatgc ccctgtttat
     3541 gcaggggcgt gtcctgctgg agcctgagcc ggagcgttat tcatctttcg ccagtggtgc
     3601 cgttccggcg gcatcacaac cgctggcgga tgatcctgac gttcgggccg tgttccgcaa
     3661 tgaggaagtg atccgtcgtg ctggtggcgt ggaatgtctt gaaagctggt tacttcgtga
     3721 aaaaggctgc cagtggcctc attccgactg gcacagcgag aacatgacca caatgcgaca
     3781 cgctccgggt gcaatccgtc tgtgctggca ctgcgataat cagctgcgcg atcagttcac
     3841 ggaacggctg gaatcaatgg caacggataa ctgtgcccgc tgggtgttgt ctgttgtgcg
     3901 tcgggatctc ggttttgatg acagtcacgt tgtgacaatg ccggaactgt gctggtggct
     3961 gattcgtaat gatctggcgg atgccttacc ggaaagtgca gcccgtaagg cactgagatt
     4021 accgaagcct gttgtgccga ctgttacccg ggaaagtgac cttgtgcctt cggttcctgc
     4081 caccagcatc atccaggata aggcgaaaaa ggtgctggcg ctgaaagtgg atccggagtc
     4141 gccggagtct tttatgttac gcccaaaacg tcgccgctgg gttaatgaaa agtacacgcg
     4201 ctgggttaag acacagccgt gtgcatgttg tggaaagccc gctgatgatc cccaccacct
     4261 gataggtcac ggtcaggggg gaatgggtac aaaagcgcat gacctttttg tgttgccttt
     4321 gtgcagaaaa caccatgacg aactgcatgc ggataccgtg gcatttgaag agaagtatgg
     4381 ctcccaactg gagctgatat ttcgttttat cgatcgcgcg ctggcgattg gtgtgctgtc
     4441 ctgattttgt ggagaaagtt gatgcgtgat attcagatgg ttcttgaacg ctggggggca
     4501 tgggctgcaa gtggtaacac cggggtggac tattctccga tcgctgccgg attcaaagga
     4561 cttttaccat ctgccactaa accacgtccg gcctgctgcg atgatgacgg acttatcatt
     4621 gaaaactgtc ttgctcgtct gaagcagaaa aaacctgagg agtattcgct tctcattgct
     4681 cattatttgt tgcgaatatc aaaaagacag atagccagga cgagaaagaa aagcgaaaaa
     4741 gcaatacgaa ttgagatgca gatagccgaa gggtttattg acggatgttt gtctgtgctg
     4801 ggggtaagac tggagatgga cgactggctg ctaaaaaagt aaaaaatgat tagtgcggtc
     4861 cgcaaaaagt atgtcagtat gttaagattg gtcactccgt catacagctt aaacctgccg
     4921 cctggcgggt tttttatgac tgaaatcgcg tcagtacagt aaacgcgctg gtggcggtga
     4981 atacctgtct ttcagcttgc tggctttttc gacaagagtt attggtgtgt cacgttaacc
     5041 ggaaaaggga aaaagacatg ctgaaacagc aggatatgac agaaaccgcc agagtggtgt
     5101 ttaatgaatt aagc
//
LOCUS       DAJTNA010000237         5058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTNA010000237 DAJTNA010000000
VERSION     DAJTNA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.242"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..1315)
                     /locus_tag="OO161_003798"
     CDS             complement(<1..1315)
                     /locus_tag="OO161_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5464683.1"
                     /translation="MRHFIYQDEKSHKFWAIEQQGNELHINWGKVGTNGQSQIKSFAD
                     AAAAAKAELKLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAV
                     IRLTDDINRFAFPHRSRPREINYLHKDGQIWQLIANNTRAYDPANNYRSYPENWQQAF
                     AELQMRVQGNQQTGSAQSDAALLWSFWNSYSADELVDDLVIRCGLESAVEIALLALQL
                     RYKPVKGAVTTIIPPNHKAESLPSWHQRLCHHLSLASEDEWQRCVDKVLAAIPTLSPA
                     RQPFAALLLPECPDIANAMALRYADQNVPAMTWLSMMVSDDVALAIQEKYGFPPLYND
                     FRKYLATLLANNGMRGVSRILLKLPVDYPVKYTDLFTHIHANAEDLVKWLWKTNHPDA
                     IQILILGVNGKKKHLEYLSKACQKHPAAAIAAYATLLAIHENNEWR"
     gene            complement(1456..3489)
                     /gene="metG"
                     /locus_tag="OO161_003799"
     CDS             complement(1456..3489)
                     /gene="metG"
                     /locus_tag="OO161_003799"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5464684.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWIHSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYRTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3621..4730
                     /gene="apbC"
                     /locus_tag="OO161_003800"
     CDS             3621..4730
                     /gene="apbC"
                     /locus_tag="OO161_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5464685.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT         1226 a         1236 c         1288 g         1308 t
ORIGIN      
        1 tacgccactc attattttca tgtattgcca gcaaagtagc ataagcggca atagccgcag
       61 cgggatgttt ttggcaggct ttgcttaagt attccaggtg ctttttcttg ccatttacac
      121 cgaggatcag aatttgaatc gcatccgggt gattcgtttt ccatagccat ttgacaagat
      181 cttcagcatt ggcgtgtatg tgagtgaaaa ggtccgtgta ttttacggga tagtcgacag
      241 gtagcttgag aagaatccgg cttacaccac gcattccatt atttgccagc aacgtagcca
      301 gatatttgcg gaaatcatta tatagcggtg ggaaaccata tttttcctgg atggccaggg
      361 caacatcatc gcttaccatc atacttaacc aggtcatcgc cggaacgttt tggtctgcat
      421 aacgtagcgc catagcattg gcaatatccg ggcattcggg gagcagtaac gccgcaaaag
      481 gttgccgtgc tggggatagc gtcggaatgg cggcgagtac tttatctaca cagcgttgcc
      541 actcatcttc tgaagcgagt gaaagatgat gacatagacg ttgatgccaa ctcggcagcg
      601 attctgcttt gtgattaggt ggaatgatgg tggttactgc gccttttact ggtttatatc
      661 taagttgaag agcgagaagg gcaatttcaa cggcactttc cagaccacag cgaatgacta
      721 agtcatcgac cagttcatct gcagagtaag agttccagaa actccagagc aatgctgcat
      781 cagattgtgc actccctgtc tgttgattac cctgaactcg catttgtaac tcagcaaaag
      841 cctgctgcca gttttctgga taagaacggt aattgttggc gggatcatat gccctggtgt
      901 tattcgcgat gagctgccat atttgaccat ctttgtgcag ataattgatc tctctggggc
      961 gagagcgatg aggaaaagca aatcgattaa tatcgtcagt tagccgaatg accgcatcat
     1021 cagccagcca ggggcgttgg tttttactct caggggaagt ttcaacttca ggagtggctt
     1081 tggtaatggg ggggatatgc acgtttgctg aagcattttc cacatagcct tttttcgttt
     1141 tctctgcgat cagcttaagc tccgcttttg ccgctgccgc agcatccgca aaacttttta
     1201 tctggctttg cccgttagtg ccgactttgc cccagttgat atgcagctcg tttccctgct
     1261 gctcaatcgc ccagaattta tgtgattttt cgtcctgata gataaagtgt ctcattctgc
     1321 atccctgtaa ttttatccag ttgaatgcag atgctaccag tatttatgcg ggttagagag
     1381 agacaaaatc gtaagaaaac aactttagga atttataaag aaaaggcgct gtcgatgcag
     1441 cgccttgaag ggggattatt tcacctgatg acccggttta gcaccagcat ccgggcttag
     1501 caggaaaata tctttcccgc caggaccggc agccatcacc atgccttcgg agataccgaa
     1561 gcgcatttta cgtggtgcca ggttagccac cataatggtg tgacgaccaa tcagtgcctg
     1621 cggatccggg taagcagaac gaatgccgga gaagacatta cgcttttcgc cgccgagatc
     1681 cagcgtcagg cgtagcagtt tgtcagaacc ttcaacaaac tctgcgtttt caatcagcgc
     1741 cacgcgcagg tcaactttgg cgaagtcgtc aaaggtgatg gtttcctgaa tcggatcatc
     1801 tgccagcggg ccagttaccg gcgcggcagc tgctttcact tcttctttag acgcttccac
     1861 cagtgcttca acctgcttca tatctatgcg gttatacagc gccttgaacg gattcacttt
     1921 atggcccagc agcggttgct ggataccatc ccaggtcagt tccgtattaa ggaatgcttc
     1981 tgcgcgctcg gtcagtttcg gcagtaccgg cttcaggtaa gtcatcagca cgcggaacag
     2041 gtttatgccc attgagcaaa ttgcctgcag gtcggcatcg cggccttcct gtttcgctac
     2101 cacccacgga gcctgttcat cgacatagcg gttagccaga tcagccagcg ccatgatttc
     2161 gcgcactgct ttaccaaatt cgcggctttc ccacgcttca ccaatcactt cagcggcatc
     2221 agtgaaggtt ctgtacaact gcgggtcagc cagttcgctt gccagcacgc cgtcaaaacg
     2281 cttgttgata aagcccgcgt tgcgggaggc caggttaacc actttgttca cgatatcggc
     2341 attcacgcgc tgaacgaaat cttccaggtt gagatcgata tcatcaatgc gcgaagagag
     2401 tttcgcagtg tagtagtaac gcaggctgtc ggcgtcaaaa tgattcagcc aggtgctggc
     2461 tttaataaag gtaccgcgag acttagacat cttcgcgccg ttcaccgtca catagccatg
     2521 aacaaacagg ttggtcggct tgcggaagtt gctgccttcc agcattgctg gccagaacag
     2581 actgtggaag taaacaatat ctttaccgat gaagtggtac agctcggcgg tggagtcttt
     2641 cttccagtat tcatcgaagc ttacgctgtc gccgcgcttg tcgcacagat tcttgaaaga
     2701 acccatatag ccaatcggcg catctagcca gacgtagaaa tatttacccg gcgcgttcgg
     2761 aatttcaaaa ccgaagtacg gcgcgtcgcg ggagatatcc cactgttgca ggccagattc
     2821 aaaccactcc tgcattttat ttgccacctg ctcctgcaac gcaccgctgt ggatccatgc
     2881 ctgcaacatt tcgctgaaag agggcagatc aaagaagaag tgttcagaat cacgcattac
     2941 cggcgtagcg ccagaaacca ccgatttcgg ctcgatcaat tcagtcgggc tgtaggtcgc
     3001 gccgcagact tcgcagttat cgccgtattg atccggggat ttacatttcg ggcaggtgcc
     3061 ttttacaaaa cggtccggca ggaacatgcc tttttccgga tcgtacagct gagagatggt
     3121 gcggttttta ataaaaccgt tttctttcag gcgagagtag ataagttctg acaactggcg
     3181 gttctcttcg ctgtgcgtcg agtgatagtt gtcatagctg atgttaaagc ctgcgaaatc
     3241 agtctgatgc tcctgactca tttcgccaat catctgctcc ggggtgatac caagctgctg
     3301 agctttcagc atgatcggtg taccgtgggc atcgtcggcg cagatgaagt tgacctcgtg
     3361 gccgcgcatt cgctggtaac ggacccagac atcagcctgg atgtgctcca gcatatggcc
     3421 gaggtggatt gagccgttag cgtacggcag tgcgcacgtc accagaattt tcttcgcgac
     3481 ttgagtcata gtaggcatta cttcttaata gtgaaaaggg gttagatagt accaaatggg
     3541 aaaatgttaa gtaagtactt ctctatattc ttcaggtaaa ctatcagctg atggtttatc
     3601 tatacaaaaa aggagtcggg atgaacgaac aatcccaggc caaatcacca gaagccttgc
     3661 gcgcaatggt cgccgggaca ctggccaatt ttcagcaccc aaccctgaag cataacctca
     3721 ccacgctgaa agcgttgcac catgtcgcct ggatggacga cacgttgcat gtggaactgg
     3781 ttatgccatt cgtctggcat agcgcgtttg aagagttaaa agagcaatgt agcgccgaac
     3841 tgctgcgtat caccggggcg aaagctatcg actggaaact gtcgcacaat atcgccacct
     3901 taaaacgcgt gaagaatcag ccgggtatta atggcgtgaa gaacatcatt gcggtcagtt
     3961 ccggtaaagg cggcgtgggt aaatcctcca cggcggtaaa cctggcgctg gcactggcgg
     4021 cggaaggggc gaaagtcggc attctggacg ccgatatcta cggcccatca attcccacca
     4081 tgctgggcgc agaaaatcag cgtccaactt cgccggacgg tacccatatg gcaccgatta
     4141 tgtctcatgg cctggcaacc aattcaattg gttatctggt cactgacgac aatgcgatgg
     4201 tgtggcgtgg accgatggcg agcaaggcgt taatgcagat gctgcaggaa accttgtggc
     4261 cggatctcga ctatctggtg cttgatatgc cgccaggcac cggtgacatt caattgacgc
     4321 tggcgcaaaa cattccagta actggtgcag tcgtggtcac caccccgcag gacatcgcgt
     4381 tgatcgatgc gaaaaaaggc attgtgatgt tcgagaaagt cgaggttccg gtgctcggta
     4441 ttgtcgagaa catgagtgtg catatttgca gtaactgtgg tcaccatgaa ccgattttcg
     4501 gtaccggtgg tgcagagaaa ctggctgaaa aataccatac acagctgctg gggcagatgc
     4561 cgttacacat ctccttacgt gaggatctcg ataaaggcac gccaacggtt atcagccgtc
     4621 cggagagcga atttaccgct atctaccgcc agctggctga ccgcgttgca gctcagctct
     4681 actggcaggg tgaagtcatt ccaggcgaga tttccttccg cgcggtctaa cgcctattaa
     4741 ccattctggt tattcttcat acaccgcaga gatgttatat tgatgcggtg tatttgtttt
     4801 tgccgcatta atgaacactt atttaatact gcgaatattc tgcggacctt cgtccgaaac
     4861 tctacaaagt cgattgatcg tacaggcaat gcttcattat catccttcgt ggctgtgtag
     4921 tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc tttactacag
     4981 ggataacgtt caatgaataa gtattggctg tcaggtaatg ctgccaactt actgatagtg
     5041 ttttatgttc agataatg
//
LOCUS       DAJTNA010000238         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTNA010000238 DAJTNA010000000
VERSION     DAJTNA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.243"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..269
                     /locus_tag="OO161_003801"
     CDS             <1..269
                     /locus_tag="OO161_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCR5464686.1"
                     /translation="IGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYAQENVTID
                     MEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(310..780)
                     /locus_tag="OO161_003802"
     CDS             complement(310..780)
                     /locus_tag="OO161_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5464687.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(827..1546)
                     /gene="yehT"
                     /locus_tag="OO161_003803"
     CDS             complement(827..1546)
                     /gene="yehT"
                     /locus_tag="OO161_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5464688.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKTLKEAIGL"
     gene            complement(1543..3228)
                     /gene="btsS"
                     /locus_tag="OO161_003804"
     CDS             complement(1543..3228)
                     /gene="btsS"
                     /locus_tag="OO161_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5464689.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHIPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3450..3515
                     /locus_tag="OO161_003805"
                     /pseudo
     CDS             3450..3515
                     /locus_tag="OO161_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            3743..3991
                     /locus_tag="OO161_003806"
     CDS             3743..3991
                     /locus_tag="OO161_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5464690.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFFH"
     gene            complement(4095..4223)
                     /locus_tag="OO161_003807"
                     /pseudo
     CDS             complement(4095..4223)
                     /locus_tag="OO161_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4308..4853
                     /locus_tag="OO161_003808"
     CDS             4308..4853
                     /locus_tag="OO161_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HCR5464691.1"
                     /translation="MNISELKKCIHYEVIGCKRPFSWRKAIVRAIKHRRSRYLFWWRI
                     AKYLFDKGGYRRKIAGKIERFILDKYNVTVPLTVNIGKGFDISYLNGVVIGHKVTIGE
                     NCSIKPGVTIGLRGEFNDMDIVIGNNVTIGCNATILGGKVHIGNNVKIGAHALVLHDI
                     PDDSTFITKFHSEIIYNSSHT"
     gene            complement(4864..>5024)
                     /locus_tag="OO161_003809"
     CDS             complement(4864..>5024)
                     /locus_tag="OO161_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5464692.1"
                     /translation="EFSEYIAPLQDAVDLEIATEEERSLLEAWNKYRVLVSRIDINTA
                     PDIVWPEL"
BASE COUNT         1401 a         1237 c         1114 g         1272 t
ORIGIN      
        1 ctattggtgc aaccaccaaa gaagacgctg ccaagacact tgagccgtta agcgccaaat
       61 acaaaaacat cgcgggtgtt gaagaaaaat taacctatac cgatacctac gcgcaggaaa
      121 acgtgactat cgatatggaa aaagtggatt ttaaagccct gcagggtatt tcaggaatca
      181 acgtttctgc tgaagatgcc aaaaaaggta tcactatggc gcaaatggaa ctggtgatga
      241 aagccgctgg ttttaaagaa gtgaaataat ctgtcggcgg ccatgtttcg catggccgcc
      301 atacccgtct tagcttttct tcacatgctg gcgcgctgcc agtccacgca gaaaataacg
      361 taaaaattga tcgccgcatt cgcggaagtt tttatgatcc ggtgcacgca tcatcgccgt
      421 aatttccggc atcgaaacgc ggaactgctg ttcggtgagg atcgccagaa tgtcatcggt
      481 tttcagcgaa aacgcgatgc gtaatttttt tagcacgatg ttgttattaa tgcgacgttc
      541 cggctccagt gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag
      601 gaatgacgac aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag
      661 ccagacggcg atctgttccg cggtggcttc gacattaccc agcgccagaa tacgcaccag
      721 gtcattatta ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttagcat
      781 gaggccttca ggtgttgatg aggcaaaaag ccattttagc agtcttttac aggccaatcg
      841 cctcttttaa ggttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa
      901 tcaactcggc ctggccgtta tcttccagac gaatctcctg taaatgcgcg aggttaacca
      961 gatactgacg atggcagcgc agtagtggtg tacgactttc cagggtacgt aatgtcaatt
     1021 cggtaaagcc ctctttccct tcgtggctgg taacgtagac accgctcatc cgactgctga
     1081 caaatgccac atctttcatt tgcagcaaat aaatccgact atgcccagta caagggataa
     1141 atttcagcgc ctgttgattt tctggtaaca gcgaaacatc ctgcttgctg cgttcctgcc
     1201 gcaaacgcgc cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa
     1261 aggcgtgttc ttcaaaggct ttaatggcgt attcgtcaaa cgcggtgaga aaaacaatat
     1321 acggacgatg ttccgggtcg agcatcccca ccatttccag accactgatg cgcggcatct
     1381 ggatatcgag aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttccacgg
     1441 cgtttgaaca ctcaccaacg atttcaatat cgctctgctc ctgcaaaaat acgcgcaggt
     1501 tctcccgtgc taacggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca
     1561 tggtagtcgt aacgttattc gggtgtaact atcaggctca caggcgacgc ttatcccata
     1621 gtcatcgcca aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc
     1681 attggttacc ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc
     1741 ctcacgtcgg gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt
     1801 aatggcgttt tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc
     1861 ttgcggaata gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag
     1921 ataagcgttc acatgttcaa tttcgtcggc gagagtaaca aactccgaag gccgctttaa
     1981 gtttttgcgg aaaaaagtgg aaagatactg caccagctgg ctggcctgtt cgctgtcgcg
     2041 acggatcacc gctttaatgg tgttaagcgc attaaacaaa aaatgggggt tcacctgggc
     2101 gtgaagcagt ttgatctctg actgggtgag catcgctttt tgccgctcat attgcccggc
     2161 gaggatctgc gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt tgattgaact
     2221 gaataaacgg tttttggctt catacaattt gatggtgccc atcacccgct gattttcacc
     2281 acgcaacgga attaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacgata
     2341 aggtacttcg ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtgta
     2401 agtcgaagat atcggtttgc cgggtatatg gtggtcgtca ccaattccgg taaaggccag
     2461 caatttctct cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagcacctg
     2521 tgccactttc atgctgttca cttcgttaaa cccctggcgc aaaatgcctt ccgtcgaggc
     2581 agccactttc agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt
     2641 atcgagcaat atacgcataa acagcgccgc gccgacggta ttggtgacca tcattggcgc
     2701 agcaatatta ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg ccaggatgat
     2761 caacatttgc accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac
     2821 tttatcagtg cgcccgcgac ggatcaggat gctgtgtacc aggccgccga gcaatccttc
     2881 aacgatggtc gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa
     2941 cccgccggtc aggccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac
     3001 cgcgcctatc gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc
     3061 catgatgcag aagatggaaa agacgatgta gcagagaaat ttatgcggca gacgaaccgt
     3121 gacctgcatt aacggtatga ataatggcgt tttactcatt aaccatgcaa tgactaaaaa
     3181 aacgcacatc tgctgaagca gcagcaacac cagattaaaa tcgtacatac ccgcaaacca
     3241 cacttccctt taaaacgcgt aacatacatt gcctgcgttt aactttcttt gaactcttgc
     3301 agaaaaatga gaattcgtga gtacaatcac tcaaaatcgc ctggcaaaaa taaaatcacc
     3361 ctataaatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
     3421 accgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
     3481 tgctttgtga tattaaccct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
     3541 gaagaccgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
     3601 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
     3661 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
     3721 actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
     3781 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
     3841 acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac
     3901 gcgcaccgcc gataaagatt cagctaaaac ttttgttcag gagactctga aagatacctg
     3961 gaagtctgct gacgagtggt tttttcacta attaacacgt aaaatcggta acggctggaa
     4021 atcattcaat actcgcacta tcgaaagttc gccagccagc cgtggcacgt tcttgcatac
     4081 gacgtgctac ggtttcattc atctccgacc ggaaacttct tatacaaagt cgatacgcca
     4141 acatcataga tgatcaccac cttctggcga gaaactcctg atgcaatttg tcgtccggcc
     4201 tgtgcctatt gctccggcgt gagagtaata cagtaaactg tagaaaaagc ccattaaacc
     4261 taatataata ataagttaca gaattatctt tactgaataa tagatatatg aatattagtg
     4321 aacttaaaaa gtgtatacac tatgaagtaa taggttgtaa acgccctttc tcctggcgaa
     4381 aagcaattgt tcgcgcaata aaacacagaa gatcacgtta tttattttgg tggcgcatag
     4441 caaaatatct ttttgataaa ggcggatacc ggcgaaagat tgcaggaaaa atagaacgct
     4501 tcattcttga taaatacaat gtaaccgttc ccttaactgt aaatattggt aaagggttcg
     4561 acatatctta tctcaatggc gttgttatcg gtcacaaagt tacaataggt gaaaattgtt
     4621 caattaagcc tggggtaact attggacttc gcggtgaatt taatgatatg gatattgtca
     4681 taggaaataa tgtaaccatt ggctgtaacg ccaccatcct tggtggcaaa gtgcatatag
     4741 ggaataatgt aaaaatagga gctcatgcat tggtactgca tgacattcct gatgactcca
     4801 cattcattac caaatttcac tctgaaatta tttataattc atctcacaca taattccgtt
     4861 taatcacagc tcaggccata cgatatccgg ggctgtattt atatcaatcc gactcactaa
     4921 tacccggtat ttattccatg cctccagcaa cgatctttct tcctccgttg cgatttccag
     4981 atctacagca tcctgcagtg gcgcaatata ctcactgaat tcct
//
LOCUS       DAJTNA010000239         5017 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTNA010000239 DAJTNA010000000
VERSION     DAJTNA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.244"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            60..1859
                     /gene="cysJ"
                     /locus_tag="OO161_003810"
     CDS             60..1859
                     /gene="cysJ"
                     /locus_tag="OO161_003810"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5464693.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLMNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLSDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            1859..3571
                     /gene="cysI"
                     /locus_tag="OO161_003811"
     CDS             1859..3571
                     /gene="cysI"
                     /locus_tag="OO161_003811"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5464694.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVDTFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3646..4380
                     /gene="cysH"
                     /locus_tag="OO161_003812"
     CDS             3646..4380
                     /gene="cysH"
                     /locus_tag="OO161_003812"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5464695.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            4645..4797
                     /locus_tag="OO161_003813"
     CDS             4645..4797
                     /locus_tag="OO161_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5464696.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         1251 a         1268 c         1402 g         1096 t
ORIGIN      
        1 tgttttatgg gcgctgacag ggcgcagaaa cagctttgct tactggaaca taacgacgca
       61 tgacgacaca ggtcccacct tccgcgttgc ttccgttgaa cccggagcaa ctggcacgcc
      121 ttcaggcggc cacgaccgat ttaacgccta cccagcttgc ctgggtttct ggctatttct
      181 ggggcgtact caatcagcag cctgctgcgc ttgcagcgac gccagcgcca gccgcagaaa
      241 tgccgggtat aactattatc tccgcctcgc aaaccggcaa tgcgcgccgg gttgctgaag
      301 cattacgcga tgatttatta gcagcaaaac tgaacgttaa gctgatgaac gcgggcgact
      361 ataaattcaa acaaatcgcc agcgaaaaac tgctcatcgt agtgacgtca acgcaagggg
      421 aaggggaacc gccggaagaa gccgtcgcgc tgcataagtt cctgttctcc aaaaaagcgc
      481 caaagctgga aaacaccgcg tttgccgtgt ttagcctcgg cgatagctct tatgaatttt
      541 tctgccagtc cgggaaagat ttcgacagca agctggcgga actgggtggt gaacgcctgc
      601 tcgaccgtgt cgatgccgat gttgaatacc aggctgctgc cagcgagtgg cgcgcccgcg
      661 tggttgatgc gcttaaatcg cgtgcgcctg tcgcggcacc ttcgcaatcc gtcgctactg
      721 gcgcggtaaa tgaaatccac accagcccgt acagcaaaga cgcgccgctg gtggctagcc
      781 tctctgttaa ccagaaaatt accgggcgta actctgaaaa agacgttcgc catatcgaaa
      841 ttgacttaag tgactcgggc ctgcgttacc agccgggtga cgcgctgggc gtctggtatc
      901 agaacgatcc ggcactggtg aaagaacttg tcgaactgct gtggctgaaa ggcgatgaac
      961 ctgtcaccgt cgagggcaaa acgttgcctc tgaacgaagc gctacagtgg cacttcgaac
     1021 tgaccgtcaa caccgccaac attgttgaga attacgccac gcttacccgc agcgaaacac
     1081 tgctgccgct ggtgggcgat aaagcgaagt tacagcatta cgccgcgacg acgccgattg
     1141 tcgacatggt gcgtttctct ccggcgcaac tggatgccga agcgctgatt aatctgctgc
     1201 gcccgctgac cccgcgcctg tattccatcg cctcctcgca ggcggaagtc gagaacgaag
     1261 tacacgtcac cgttggtgtg gtgcgttacg acgtggaagg ccgagcccgt gccggtggtg
     1321 cctccagctt cctcgcggat cgcgtggaag aagagggcga agtccgcgta tttatcgaac
     1381 ataacgataa ctttcgcctg cccgctaacc cggaaacccc ggtgattatg attggcccag
     1441 gcaccggtat tgcgccgttc cgcgccttta tgcagcaacg cgccgccgac gaagcgccag
     1501 gtaaaaactg gctgttcttt ggtaatccgc actttacgga agacttcctg taccaggtgg
     1561 agtggcagcg ctacgtcaaa gatggcgtgc tgacacgtat cgatcttgcc tggtcgcgcg
     1621 accaaaaaga aaaagtttac gtacaagaca aactgcgcga acagggcgcg gagctgtggc
     1681 gctggatcaa tgatggtgcc cacatttatg tctgcggcga cgctaatcgc atggcgaaag
     1741 acgttgagca ggcacttctg gaagtgattg ccgaatttgg tggcatggac accgaagcgg
     1801 cggatgaatt tttaagtgag ctgcgcgtag agcgccgtta tcagcgagat gtctactaat
     1861 gagcgaaaaa catccagggc ctttagtggt cgaaggaaaa ctgacagacg ccgagcgcat
     1921 gaagcttgaa agcaactacc tgcgcggcac cattgcggaa gatttaaacg acggtctgac
     1981 cggcggcttt aagggcgaca acttcctgct gatccgcttc cacggcatgt atcagcagga
     2041 tgaccgcgac atccgcgccg aacgtgctga acagaagctg gagccgcgcc acgcgatgct
     2101 gcttcgctgt cgtctgccgg gtggggtgat taccactaaa cagtggcagg cgatcgacaa
     2161 atttgccggt gaaaacacca tctatggcag cattcgcctg accaaccgcc agacattcca
     2221 gttccacggc attctgaaaa agaacgtcaa accggtgcac cagatgctgc actcggtcgg
     2281 tcttgatgcg ctggcgacag ctaacgacat gaaccgtaac gtactctgca cctcgaaccc
     2341 ttacgagtcg cagctgcacg cggaagcgta tgagtgggcg aagaagattt ctgagcatct
     2401 gttgcctcgt acccgcgcgt atgcggagat ctggctcgac caggaaaaag tcgccactac
     2461 tgatgaagaa ccgatccttg gtcagaccta cctgccgcgt aagttcaaaa ccacggtagt
     2521 gatcccgccg cagaacgata tcgatctgca tgccaacgac atgaacttcg tggcaatcgc
     2581 cgaaaacggc aagctggtag gcttcaacct gctggtgggc ggtgggcttt ctatcgaaca
     2641 cggcaacaag aaaacctacg cccgcacggc gagcgagttt ggctatctgc cgctggagca
     2701 tacgctggcg gtggccgaag ccgtcgtgac aactcagcgt gactggggta accgaaccga
     2761 tcgtaaaaat gccaaaacca aatacacgct ggagcgcgtg ggggttgata cgtttaaagc
     2821 ggaagtggag cgtcgcgcgg ggatcaaatt tgaaccgatc cgtccatatg agttcaccgg
     2881 acgaggcgat cgtattggct gggttaaggg cattgatgat aactggcacc tgacgctgtt
     2941 tatcgaaaat ggtcgcatcc ttgattatcc ggggcgtccg ctgaaaaccg gcctgctgga
     3001 gatcgcgaag atccacaaag gcgatttccg cattacggcg aaccagaatc tgatcatcgc
     3061 cggtgtaccg gaaagcgaga aagcgaagat cgagaagatc gccaaagaga gcgggttaat
     3121 gaatgccgtc acgccgcagc gtgaaaactc gatggcctgc gtgtcattcc cgacttgccc
     3181 gctggcgatg gcggaagcag agcgtttcct gccgtctttt atcgacaaca tcgataattt
     3241 aatggcgaaa catggtgtca gcgatgagca tatcgtgatg cgtgtaacag gctgcccgaa
     3301 cggttgtggt cgcgcgatgc tggcggaagt gggcctggtg ggtaaagcgc cgggtcgcta
     3361 caacctgcat cttggcggca accgcattgg gacacgtatc ccacggatgt ataaagaaaa
     3421 catcaccgag ccggaaatcc tggcgtcgct tgatgaactg atagggcgct gggcgaaaga
     3481 gcgcgaagcg ggtgaaggct tcggcgactt tacggtgcgt gcgggcatca ttcgcccggt
     3541 gctcgatccg gcgcgcgatt tgtgggatta accatcagcc cggtcctgta ggcctgataa
     3601 gaacgcgtga gcgtcgcatc aggcaaggca aacagtgagg aatctatgtc caaactcgat
     3661 ctaaacgccc tgaacgaact gccgaaggta gatcgcattc tggcgctggc ggaaactaac
     3721 gccgaactgg aaaaactgga cgctgaaggc cgcgtagcct gggcgctgga taatctgccc
     3781 ggtgaatatg tactttcttc cagctttggc attcaggcgg cggtgagcct gcatctggtg
     3841 aatcaaattc gcccggatat tccggtgatc ctcaccgata cgggttactt gttcccggaa
     3901 acctaccgct ttattgacga gttaacggac aaactcaagc tcaacctgaa agtgtaccgt
     3961 gctaccgaaa gcgcagcctg gcaggaagca cgctacggaa aactgtggga gcagggcgtt
     4021 gaaggcattg aaaagtacaa tgacatcaac aaagtcgaac cgatgaaccg ggctctgaaa
     4081 gaacttaacg cgcaaacctg gtttgctggc ctgcgccgtg aacaatccgg cagtcgcgcc
     4141 aatttaccgg tgctggcaat tcagcgtggc gtatttaaag tgctgccgat tatcgactgg
     4201 gataaccgaa ctatttatca gtacctgcaa aaacatggcc tgaaatatca cccattatgg
     4261 gatgaaggat atttatcggt cggtgatacc catacaaccc gtaaatggga acctggcatg
     4321 tcggaagaag aaacacgttt ctttggctta aaaagggaat gtggattgca cgaagggtaa
     4381 atattgccgg ataaattaaa cgcctctgtc agaaatgatg ggggcgtttt tattttgcga
     4441 gcagtattcc tgattttaaa cgtgaacgcc gctggaagaa ttatcaggta tagaatggcg
     4501 tcgggtgctt gaggctgtct ggcgttaagc attcgcgagg ttccagatgg acaaaagccc
     4561 caggcgatat ttctatcaac ctgaggccag cgttcgaacc caaacaattc gaatgttagt
     4621 ctcttctttg cagggaggca agacatgctg acaaaatatg cccttgtggc aatcatagtg
     4681 ctgtgtttta cagtactggg attcacgctg atggtaggcg actcgttgtg tgagttgagt
     4741 atcagagaac gtggtatgga gtttaaggca gttctcgctt acgaatcgaa gaagtagcca
     4801 ccacgcgggg ataaaacccc gcgtactgaa ttgtgatggt ttggtctcct gcaccctttt
     4861 ttaatttata ttgtttatgt gaatattatg cctatgttta ttattcatat atattacgga
     4921 tgaaaattat ttgattattc ataatgtaaa tattttattg aatgatatat atcacagata
     4981 atttttaata tatgcgtaaa tatcctttaa gtttact
//
LOCUS       DAJTNA010000240         4954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTNA010000240 DAJTNA010000000
VERSION     DAJTNA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.245"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            241..807
                     /locus_tag="OO161_003814"
     CDS             241..807
                     /locus_tag="OO161_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5464697.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            858..3317
                     /locus_tag="OO161_003815"
     CDS             858..3317
                     /locus_tag="OO161_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5464698.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGVYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRSEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASNDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSTAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDHDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRHSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDNQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3332..4063
                     /locus_tag="OO161_003816"
     CDS             3332..4063
                     /locus_tag="OO161_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5464699.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            4060..4954
                     /locus_tag="OO161_003817"
                     /pseudo
     CDS             4060..4954
                     /locus_tag="OO161_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
BASE COUNT         1384 a         1205 c         1121 g         1244 t
ORIGIN      
        1 aaatcgatga tttttaatca tctatcttcc tctcagttac atgaataaac caccaaatat
       61 agcgcacccc tataatttcc aatactttat tcatcaaaca tttaattcat accatcaatt
      121 aattactttt acatttaata aaattaataa tatttttaag aaaaactaaa aacattcgac
      181 aatcaaataa gatattcttg caccgatata cttttttctt ttctgttaaa ggactcagtt
      241 atgtttaaag gacaaaaaac attggccgca ctggccgtat ctctgctgtt cacagcacct
      301 gtttatgcgg ctgatgaagg atccggtgaa attcacttta aaggtgaagt tattgaagca
      361 ccgtgtgaaa tacatcagga tgatattgat aaagaggttg aactcggtca ggtgaccacc
      421 agccacatta atcagtcaca tcacagcgat gccgttgctg tcgacctgcg cttagtcaac
      481 tgtgatctgg aaaactccag caacggttcc ggtggcaaga tttctaaagt tgcagtcacc
      541 tttgatagct cagcgaaaac caccggcgca gatccgattc tcaacaacac cagcacgggt
      601 gaagccaccg gcgtcggcgt acgtttaatg aataaagatc aaagcaatat cgttttaggc
      661 accgctactc cagatatcga cctggctccg acctccagcg aacaaacgct gaatttcttt
      721 gcctggatgg aacaaattga tcaggctaca cctgtaacgc caggcgcagt tacggcgaat
      781 gcaacctacg tgctggatta taaataagta ttactttggc ggcgctatcg ccgcctttca
      841 taaaatattt atcaggtatg gacaccgtga atatttatcg actctctttt gtatcctgcc
      901 tggtcgtggc gatgccttgc gcattggcgg tcgaattcaa ccttaatgtt ctcgataaat
      961 cgatgcgcga ccgcattgat atttcattat taaaggaaaa aggagtcatt gctcccggtg
     1021 tgtattttgt tagcgttgcg gtaaataata accaaatcag taacgggcaa aagattaact
     1081 ggcacaaaaa tgacgataaa accattccgt gcatcaatga tttactggtc gataaatttg
     1141 gcttaaaacc tgaagtccgt cagtcgttac cattgataaa tcagtgcgtc gattttagct
     1201 cccgatctga aatgctcttc aatttcgatc aagccaatca gcaactgaat attagtattc
     1261 cgcaagcctg gctggcgtgg cactcagaaa actggacccc accctccaca tggaaagaag
     1321 gtgtcgccgg tgtcctgatg gattacaact tgtttgccag cagctaccgc ccacaggatg
     1381 gcagcagcag cactaatctt aacgcctatg gaacagccgg aatcaacacg ggggcatggc
     1441 gcttacgcag tgattatcag ttgaatcaga ctgatagcga tgataaccat gaacagtcag
     1501 gcgaaatatc acgtacctat ctttttcgcc cgttaccgca attaggttca aaattaaccc
     1561 tcggcgaaac ggattttagt tccaatattt tcgacggttt ttcttatacc ggtgcggcac
     1621 tggcaagtaa cgatcgaatg ttgccatggg aactacgcgg ctacgcccca caaattagcg
     1681 gtattgcaca gaccaatgcc acggtgacga tcagtcaatc aggccgcgtc atttaccaga
     1741 aaaaagtccc accaggccca tttatcattg acgaccttaa tcagtctgtt cagggcacac
     1801 tggatgtcaa agtgacggaa gaagatggtc gggtgaacaa tttccaggtt tcggcagcat
     1861 cgacgccctt cctgactcgt cagggacagg ttcgctataa actggccgcg ggtcagccac
     1921 ggccctccat gtcacatcaa actgaaaatg aaaccttttt tagcaatgaa gtttcctggg
     1981 ggatgctgtc aaacacctcg ctgtacggcg gcctgctgct ttctggtgat gactaccatt
     2041 ctaccgcaat gggtattggg caaaatatgc tgtggcttgg tgcgctgtcg tttgatgtca
     2101 cgtgggccag tagccatttt gatactcagc aggacgagcg gggcttaagc taccgtttta
     2161 attacagcaa acaagtggat gccactaaca gcacgatttc cctcgccgct tatcgtttct
     2221 ccgatcgtca ttttcacagc tacgccaact atctggatca caaatacaac gacagcgatg
     2281 cgcaggacga aaaacagacg atcagcttat ctgtgggcca accgattacc ccactaaacc
     2341 tcaatcttta cgccaacctg ctacatcaaa cctggtggaa tgcagacgcc tccacgaccg
     2401 ccaacatcac ggcaggtttt aatgttgata ttggtgactg gagagatatc tctatttcga
     2461 cgtcattcaa tacaacccat tacgaagata aagatcacga caaccagatt tacctgtcga
     2521 tttcgctccc cttcggtaac ggtggtcggg ttggctatga catgcaaaac agtagccaca
     2581 gcaccacaca ccgcatgtcg tggaacgata cgctggatga acgtaatagc tggggcatgt
     2641 ctgccggact gcaatccgac cgtcctgaca atggagccca ggtgagcggc aactatcagc
     2701 acctgagttc agcgggtgag tgggatattt ctggtaccta tgccgccaat gattacagtt
     2761 ccgtcagcag cagctggagc ggttctttca ccgcaaccca atatggtgca gcgtttcatc
     2821 gccacagctc caccaatgaa ccacgcctga tggtcagcac cgatggcgtg gcagatattc
     2881 cggttcaggg caatctcgac tacaccaacc attttggcat tgcggtggta ccgttgattt
     2941 ccagttacca gccttccacc gtggcggtga acatgaatga cttacccgac ggcgtaacag
     3001 ttgcagaaaa cgttatcaaa gaaacgtgga ttgaaggcgc gataggttac aaatcactgg
     3061 cttcccgttc cggtaaagac gttaacgtca tcattcgcaa cgccagcggt cagtttcctc
     3121 ccctcggagc ggatatccgc caggatgaca gcggcattag cgtggggatg gttggcgagg
     3181 aaggacatgc ctggttaagc ggagtcgctg aaaatcaaaa gtttaccgtg gtctggggtg
     3241 ataaccagca ttgctcgctc catcttcctg aacatatgga agacaccgca aatcgcctga
     3301 ttttaccttg tcattaatta aggaaatgaa aatgacattt atgaaaggac tccccctttt
     3361 gttgttggtt gccagtctgt gcagccacgc tgcactacaa cccgatcgca ctcgtattgt
     3421 gtttaacgct aatgataaag ctaccagtct gcgggtggat aatcgtagcg ataaactccc
     3481 ctacctcgct tattcctggc tcgaaaatga aaaaggggag aaaagcgatg atcttctggt
     3541 cgccctgccg ccgatacaac gtctggaacc gaaagcaaca acgcaggtac ggatagtaaa
     3601 gcaagcctcg accacaaaat taccgggcga tcgcgaaacg ctatttttct acaatatgcg
     3661 tgaaattcct cccgccccgg aaaaaaacag cgaccatgcc gttctgcaag tcgccattca
     3721 gagtcgtatc aaggtgttct ggcgaccggc tgcattacgc aagaaagcag gagaaaaggt
     3781 cgaactgcag ttacaggtca gccagcaggg caaccaacta acactgaaaa atcctaccgc
     3841 gtattatctg accattgcct acctgggacg aaatgaaaaa ggcgttctcc ccggcttcaa
     3901 aaccgtgatg gttgcgcctt ttagtacggt caacacaaac accggaaatt atagcggcag
     3961 ccagttttat ctcggttata tggatgatta cggcgcgtta cgcatgacga cgctcaactg
     4021 cagcggacaa tgccatttac aagccgtgga ggcgaagaaa tgagtgctgg caagggagtg
     4081 ttgctcgtca tctgtctgtt atttctgccg ttgaagtctg cgcttgccct gaactgctac
     4141 tttggttcat ccggtggctc ggtagaaaaa tcagaagcga ttcaaccttt tgctgtacca
     4201 ggcaatgcca aactcggcga taagatttgg gaatctgacg atattaaaat tcccgtctat
     4261 tgtgacaaca ataccaacgg caattttgaa agtgagcatg tttatgcctg ggtaaacccc
     4321 tacccggggg tgcaggatcg ctattatcaa ctcggcgtaa cttacaatgg cgttgattat
     4381 gacgccaacc agggaaaaag ccgcatcgac actaaccagt gtattgacag tagaacattg
     4441 acatttatac ccctgagcag attatcgcga tgggatggca gaacaaaatt tgctcgggtg
     4501 atcccgctaa tattcacatg tcgcgtacct ttctcgctcg catgcggtta tacgtcaaaa
     4561 tacgagaaat gccgccgcat gattatcaaa gtacgcttag cgactatatc gtcgtgcaat
     4621 ttgacggtgc cggtagcgtt aacgaagacc ctactgccca aaacctgaaa tatcatatta
     4681 ctggtctgga aaacattcgc gtgctggatt gcagcgtcaa tttttccatt tcaccggaaa
     4741 cacaggtgat tgattttggt aaatttaatt tgctggatat tcgtcgccac acaatgtcga
     4801 aaacattcag tattaagacg acaaaatcac aaaatgacca atgcaccgac ggatttaaag
     4861 tcagttcctc cttttatacc gaagaaacgt tggttgaaga agataaagca ttgttgattg
     4921 gtaatggtct gaagctacgt ttgctggatg aaaa
//
LOCUS       DAJTNA010000241         4900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTNA010000241 DAJTNA010000000
VERSION     DAJTNA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.246"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            150..266
                     /locus_tag="OO161_003818"
     CDS             150..266
                     /locus_tag="OO161_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5464700.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            630..1472
                     /locus_tag="OO161_003819"
     CDS             630..1472
                     /locus_tag="OO161_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5464701.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1475..2563
                     /gene="capU"
                     /locus_tag="OO161_003820"
     CDS             1475..2563
                     /gene="capU"
                     /locus_tag="OO161_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5464702.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2568..3518
                     /gene="virK"
                     /locus_tag="OO161_003821"
     CDS             2568..3518
                     /gene="virK"
                     /locus_tag="OO161_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5464703.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQKL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVITRLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQDLN"
     gene            3583..4527
                     /gene="lpxM"
                     /locus_tag="OO161_003822"
                     /gene_synonym="msbB"
     CDS             3583..4527
                     /gene="lpxM"
                     /locus_tag="OO161_003822"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5464704.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRYNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(4681..>4900)
                     /locus_tag="OO161_003823"
     CDS             complement(4681..>4900)
                     /locus_tag="OO161_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5464705.1"
                     /translation="QSWLKENPKFRLIYQPVYSPWVNHAERLWQALHDTITRNHQCRS
                     MWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT         1393 a          938 c         1124 g         1445 t
ORIGIN      
        1 gttatcggtg gcaatagcct gtgcaaattt tttataaccc gggatcgtgt caggattcag
       61 agtatgataa agacagattt tcatggctgc cggtttccgt cgtgcaaaaa acatgctgat
      121 tttacgaagg aatgccttac tgaacaacct tgcccggaat accccccgaa atcagatgga
      181 acaatcagaa aaatgctctg cagttatgga ataaagaagg tggggtggta cgccgggagg
      241 gcgatccgct ggaaatggcg cagtagctgc aggcctgtca tgatgccgga atagaagttc
      301 gtgtacaggt taatgaaagc gtcaccccgt aaataaactc tgcgctgaaa ttcctttttt
      361 tcattggagc acctgtgttg ttctgaggtg aacatatcac ctctgttcag gtagccaaat
      421 tcagtgtgcc acctcaaggt tcttagtgag tcgtttttaa aagatatgat aatatatcat
      481 tgtttatgaa ttgttgatgt ttaatttagt aaaatatatc aagtatgcca tgggtttgct
      541 atgttattca tccagtatct ggaaataaaa tgcaccagct ttattgggtt ttccttttgt
      601 ctctgcttac taggtgccag aaactttaaa tgttaaacga agggggcatt ttgttcaaag
      661 ccaatcatgt tccggtatta atgtatcatc atgtcagtca ttgccccgga ctggttacac
      721 tttctcccgt gacattccgg aagcaaatga aatggttggc tgaaaacaac tggaaaacac
      781 tgtcatcaga tgaactggag tttttttatc ggggcggaaa actcccccgg aaaagcgtta
      841 tgttgacatt tgatgatgga taccttgaca actggtttca ggtatatcct ttattaaaag
      901 agtttaatct caaagcacat atttttttaa ttaccggttt tattgggaat ggtccggttc
      961 gtcactctcc tggaaaggaa tattctcatc gcgattgcga acatcaaata gcgacaggga
     1021 atgctgataa tgttatgctt cgctggtctg aagtaaacga aatgttgcaa tctggtctgg
     1081 tcgaatttca tgttcatacg catactcata cacgctggga taaaaagttc agttcacgag
     1141 aagaacagtg taaacatctt cggcaggact tgttgagcgg gagagaatat ttgaaagaaa
     1201 tgacaggaaa atgcagtaaa catttgtgtt ggccggaagg ttattataac aaggattata
     1261 ttcaggtcgc tgaagaactt ggattctatt atttatatac aacagaaaga agaatgaatg
     1321 ctcctgctaa aggaactaca cggataggaa gaataagcac caaagaacgg gaaagttgcg
     1381 catggctgaa aagacgactt ttttattata caacaccgtt tttttcatcg ttgctggcct
     1441 ttcataaggg gccaagatta ccggatgatt aataatgaat atactattta cggaatcatc
     1501 accgaatata ggtggtcagg aattacaggc tgttgctcaa atgaaggccc tgaagaaaat
     1561 ggggcattca gttctgcttg tctgcaggga aaacagcaaa attgcttttg aagccagtaa
     1621 attgggaatt gatatcacat tcgcgttatt tcgaaacagt cttcacatcc ctactgcatg
     1681 gagattactc ggaatagttc atggttttca gcccaatgca atcgtttgtc acagtgggca
     1741 tgatagcaat attgttggtt tagtacggtt attcacttgg aaacatccat tcagaattat
     1801 caggcaaaag acatatttga cacgaaaaac aaaagttttt tcaataaatc atttttgcga
     1861 tgaggtgatt gttcccggaa caagtatgaa gacacatctg gagcaggaag gatgtcgaac
     1921 ccgggttact gttgtgcctc caggctttga tttccagaaa ttatacgttg attcccgaaa
     1981 cagtttgcct ccaaatgttc tttcttggct ggcgtcccga aggggatgcc ccgttattgc
     2041 tcaggtagga atgttgcgcc cggaaaaagg gcacgaattt atgttgaatt tactgttcca
     2101 tttaaaaatg aatggacgac agttctgttg gttgattgtg gggtctggtt cgcctgaact
     2161 gcgggagcat ttacagtatc agattgacag tatgggcatg catgatgatg tttttattgc
     2221 tgacaatgtt tttcctgccg cccccgtata tcgggttgcc agtctggtgg ttctgccttc
     2281 agaaaacgaa tcgtttggta tggtactggc agaagcatcg gcattttctg tgcctgtact
     2341 ggccagtcag attggtggaa tccctgatgt tattcagaac aaccagaccg ggacattgtt
     2401 accagcaggt aataagcacg catggatgtg cgccctgaat gattttttta atgaccctgg
     2461 gcgtttttat cagatggctc gccaggcaaa acaggatata gaagagcggt ttgatattaa
     2521 taaaactgcg ttaaaaatac tcacattagc gaagcacaag taacaatatg ttttctgtaa
     2581 gtaacttatc atttatcggt ttccttaaaa ggattgtttt ttcctcagat tcactcccgg
     2641 ggaagtggga acacagaaaa ttccggttca tgtacatttt gcgatgtgct ataaatccgg
     2701 ttgccagtat tcgatattat tacgaactgc gttccttgcc gtgcattgag gatattctgg
     2761 caatacaccc cacgttgcca gcaagaattc atcgccctta tttgcataaa ggggggcgcg
     2821 cctggacccg tggacaatat attcttgagc attatcgctt tgtgcaaaaa ttgccagaaa
     2881 aatattctaa atttttgttt ccacaaaaat cggtatcatt agtccaattc atcgggaaag
     2941 atggagagaa ttttgatatc cagtgttctc ccagtggttt tgaccgggaa ggagagttga
     3001 tgttgtcatt gttttacaat aaaacagtta ttacccggtt aacattttca gtcattctga
     3061 ctcaaaatgg acacattgct tttattggcg gattacaggg agcccctaaa aataccggac
     3121 ctgatgttat tcggtgcgca acccgggcct gttatgggat tttcccaaaa cggattattt
     3181 ttgaagcatt ttgtgcgttg atgaaagctt gcaatatctc tgagtgtctg gcagtcagtg
     3241 agcacagtca tgttttccga caattgagat actggtatca gaaacgcaaa acctttgttg
     3301 cggtctatag tgatttctgg gagtctgtag caggaaaaac ctgtggtgac tggtacagat
     3361 taccaaccca ggtgattcga aagccgctaa gcgatatagc cagtaaaaaa cgctctgagt
     3421 accgaaaacg atacgctttg ctggattata tccatgaaac tactatccgc tctttggatg
     3481 catatcctgt acactcagaa catcaggatt taaattaagg tttgatgtat tctctgaata
     3541 aaacagtaat gataatattg gtagaggcat tcgcactaaa taatgaaaaa atataaatct
     3601 gagtttattc ctgaatttaa gaaaaattat ctttcccctg tttactggtc tacatggttc
     3661 cttttgggaa tgattgcagg tatttcaatg tttccccctt cattcagaga tcctgtcttg
     3721 gcaaaaatag ggcgttgggc gggcagattg agcaaaaaag ctcgtcgcag ggcgacgatt
     3781 aatttatcgc tttgtttccc agaaaagagt gatacagaac gggaaataat tgtcgacaaa
     3841 atgtttgcca cagcattgca atccatagtg atgatggcag aactggcaat tcgtggtccg
     3901 gaaaagttcc agaaacgagt gttctggaaa ggcctcgaaa ttcttgagga aattcgatac
     3961 aataacagaa atgtgatttt tctggttccc catggctgga gcgtggatat tcctgcaatg
     4021 ttgctggcag ctcaggggga aaaaatggcc gccatgtttc atcagcaacg aaatccagtg
     4081 attgattatg tctggaattc tgtacggcgt aaattcgggg ggcgcttaca ttcccgtgag
     4141 gatgggataa aaccatttat tcagtcagta cgccagggat actgggggta ttaccttcca
     4201 gatcaggatc atggtcctga atacagtgaa tttgctgatt tttttgcgac ctataaagcg
     4261 acattaccaa ttattggacg tctgatgaac atcagtcagg ctatgattat accgcttttc
     4321 ccggtttatg atgaaaaaaa acatttcctg actattgaag ttcggccacc aatggatgca
     4381 tgcattgcca gcgcggacaa taaaatgatt gcccgacaaa tgaacaaaac agtggaaatt
     4441 ttggtggggt cacatccgga acagtatatc tgggttttaa aattgttaaa aacgcgcaaa
     4501 tcaaacgaag cggacccgta cccttgaaat tataaagtac aggtatttcc actagttgtt
     4561 tcttacaggt taccaatcga aacacatccc ccttccgtgt gttgcccgtt tggcacggag
     4621 gactgtgtag atgcagttcg ttaaatttaa tttatgccta aatagctgcg cttaataccg
     4681 ctacactttt gccagtccat gtttgcctcc ggggaatggg ctgacggttt ccataaaatg
     4741 gcgaactttt ttcaacagtt gccacattga gcggcactga tgattacgcg ttattgtgtc
     4801 gtgaagtgcc tgccatagcc gttcagcatg attcacccat ggcgagtaaa ccggctgata
     4861 aatgagcctg aacttcgggt tctccttcag ccagctctgt
//
LOCUS       DAJTNA010000242         4880 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTNA010000242 DAJTNA010000000
VERSION     DAJTNA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.247"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..275)
                     /locus_tag="OO161_003824"
     CDS             complement(<1..275)
                     /locus_tag="OO161_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464706.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQT"
     gene            complement(272..646)
                     /locus_tag="OO161_003825"
     CDS             complement(272..646)
                     /locus_tag="OO161_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5464707.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(693..1067)
                     /locus_tag="OO161_003826"
     CDS             complement(693..1067)
                     /locus_tag="OO161_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5464708.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(1230..1451)
                     /locus_tag="OO161_003827"
     CDS             complement(1230..1451)
                     /locus_tag="OO161_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5464709.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(1514..1990)
                     /locus_tag="OO161_003828"
     CDS             complement(1514..1990)
                     /locus_tag="OO161_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5464710.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(2006..2479)
                     /locus_tag="OO161_003829"
     CDS             complement(2006..2479)
                     /locus_tag="OO161_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5464711.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(2821..3639)
                     /locus_tag="OO161_003830"
     CDS             complement(2821..3639)
                     /locus_tag="OO161_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5464712.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(3794..3952)
                     /locus_tag="OO161_003831"
     CDS             complement(3794..3952)
                     /locus_tag="OO161_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5464713.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(4023..>4880)
                     /gene="ag43"
                     /locus_tag="OO161_003832"
     CDS             complement(4023..>4880)
                     /gene="ag43"
                     /locus_tag="OO161_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5464714.1"
                     /translation="HDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLWADIVAQGTRHSMKASSD
                     NNDFRARGWGWLGSLETGLPFSITDNLMLEPQLHYTWQGLTLDDGQDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTKHRVSELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYN
                     GQATLNVTF"
BASE COUNT         1082 a         1298 c         1393 g         1107 t
ORIGIN      
        1 ctggtctggt gtgacagcga gtacaaccag gcgttgtccg tttccgtgat atgtgcggta
       61 ctgcagcgta tgccggtaag ggtagcaaat ggtggcgtgc attctgctga ggtctggtca
      121 gattcatcaa caacacgaag ggagtagccg ttatcgcaga ccatgttaat cagttcagcg
      181 agctcaatac catcgacgtc aacgacaatg cgccctatgt tcagtgcctg tacgttaacg
      241 ctgtcggctt ccagtgtcag ggccagtttc atcatttcgc ctccggatac ttacccaggg
      301 taatgttatt taccgttctg taattgtcgc gggtcatcag gccggtcgcc cggcgagccc
      361 ggaggatatc aatactgttt attaactgag agcggggaca ggtgctgaat cccggctggt
      421 cggtacgcac cagcgcgtat ttttccacga gaaaattaac tgcatcacac agtgaaatgc
      481 ctgcctcaat atgctgctca atcacacgtt catcgacaaa cggtgtgtca ttcagtgtga
      541 ggccataatg ctggtccagc agtcggatga gcagtgtctg ccagatggtg acgggagacg
      601 ggcagcgaga tacctcccgt acatgtgtgt cggataatgt tttcatctta agttctctgt
      661 aactggtagt gctaaaggtg agagcttcgg ggttatgagg tggttgcggg tgctggtgtc
      721 ggataaacag ccagataaac gtaaccacag gagccgaggg tgtcggcttc gcaggtcagc
      781 ccttttgcat acagcgtgac ggtatgctga tatcggggat tcagttcacc gctggtgagc
      841 atgagttcca gttgtttcat cagcagcgga aaggcctggt ccagatggta tgcatctgcg
      901 tcgctgaagc ggcctctgat accggcgcgg tcggcaaggt aatacaggcg gttaccctct
      961 tgcaccagac gagcgccaaa acagggcctc actgtgcagg gaagccccca ccaggggcgg
     1021 tcgtgattgt catcgggatg agttatcccg gagagtttgt ctgacacgat aaaatcctct
     1081 ctgaaaagtg gttaagaatg gtttaacgat gacaggtcag ccggcgcgct gaccggtttc
     1141 ggtgaagatg tatgcattga tgagaatggc ctcatccaca gcggcgtctg aggtcatctg
     1201 gtcgtactcg tttcccgggt gacggaacat cagttcaggg tgacactcat cagccggaca
     1261 tacgggccaa aactgtcctt acggcgttct gcaaacacgg caagaacgcc gggaatatcc
     1321 tgcacttcac gaccagtata cgcttcagca ctgccgtgcc agcgatactt accggtacag
     1381 aacggaaaaa gacgggatgc aggatgctgt tggtgaatac gcatggcttc accacgggtg
     1441 atgattctca tgatgtgata cctctgaaga cagaagataa aagtgaaaac aggtgtgatg
     1501 tggttgtgac gggttaaagc agaccatgtt cggcaaagga gaaaacctgg ttgccaccga
     1561 ctatcagatg gtccggcacc cggatatcca ccaggcccag tgcctgtacc agacgttcgg
     1621 tgataagccg gtctgcctta ctgggtgtga cttcaccgga cgggtgattg tgcgccagca
     1681 ccacggcagc ggcattgtgg tacagggcgc gtttaatcac ttcccgggga tgaacttccg
     1741 tacggttgat ggtgccggtg aagagggttt caccggcaat cagctgattc tggttgttca
     1801 gatacagcac ccggaactct tcccgctcca gtcccgccat gttcagaatc agccattccc
     1861 gtgccgcacg ggtggaggtg aaggccacgc cgggttcatg aagatggcgg tccagggttt
     1921 gcagggcccg cagaatgaga ctgcgctcgc cgggcgtcat gtttccgggc agaaaggaaa
     1981 gctgctgcat tgtccctgcc tccattcagt cgatgatgcg cataatggcg ctgcattccg
     2041 gatgctgcag ggcgtaatcc cgcagccggt aataatggac cgtcatggca taacactccg
     2101 tacgacaggc atgatgactg tacgtcatca gacaggcagc aatgccggcg gcttccgggc
     2161 tcatttcagc gcggttaccg ttcatggcat tgaacagtac ccatgtttcg tcatcatcgt
     2221 catccggttc gggtgccata aatgccccgc cgttgttcag ggtgtacaga ttccagatac
     2281 caccgcagta gtcttcacac agacggtcca tccagccgaa gacacggggt tccagggtga
     2341 cccactgtgg aatgaggcca aaatgctgcg gccagaagct gatgcgctgt tcatcaggga
     2401 cgggagtggc aaccagttgt tgcaggccag tctccggtgc acaggccgaa gcggtggtgt
     2461 tctgagacaa tgttttcatg tatgaaatcc ttgtaatgtg aataaatgag agggagtaaa
     2521 ggtcgggagc tggcaacatc acgttcatca ctgaccgtga tgacccggta atatcaggga
     2581 tgaatgatat tcggctgaaa ttccggacag ccgtacgcgt aaccgttccg gtcacagacg
     2641 gatggccaga tgccattgcc tgtatactca gtctgcacag actgatttct ccgggacggt
     2701 aaagcagacg ggtacaccag gcgcacaggt catcatcaga aatggaacga caccccggca
     2761 gcgcctccgg ggcgtcgggt gattctggtg cctgaatcac gccttcaggg acatgctgca
     2821 tcagcgcagg ctctccagca gcgtttctgc cattacccac aatgcgcggt tgagcttaat
     2881 atcggtgtcg atgttgtgaa tggcacgggt gtggatacgt tttccttttg cactgcggcc
     2941 ggaaatcccg cctttcagca tattctcctg gatggtctga tatgcgctcc acaggtcttt
     3001 accgtaatcc tccctgcgtc gtggtgtcag aatgtcagcg gtggtgactg gctgatgttc
     3061 gtcaccataa cggtaagtca gtgccgcctg tgccagcgcc tggcgtgccg gtggcggcag
     3121 gaccagcgac tgcatggcat cacgcttttc ctcaatccgg tcaaaaaccc ccaccacttc
     3181 gtaagcccct tcgataactt tctccaccac atttccccgg tgtggaacac gcacttcccc
     3241 cagagactga ccgcagacgc agccattctg gcagacgaat ctgaagtaac ccggcagcat
     3301 ctggtagctg gaggtaccgt catgagagtt gagcagaata atttcaggga catgttctcc
     3361 gtttatctct ccggcccgcc gcagacgcag catgtgtttt gtgtatcccc ggcggcccgg
     3421 gtcgcgcacg cgggtctggc aggcgaagaa tggctgaaaa ccttcctgct gcaggctttc
     3481 cagtacggtg atggtgggaa tgtacgcata tcgtttactg cgggaagtat gccggtcttc
     3541 accgaaaata ctgggaacat agtgcatcag ttcttcgtgt gtcagcggac ggtcacggcg
     3601 tatctggttc gcataaccaa aacgactggc tagtcgcata atttgctcct tatcggtggt
     3661 tacgatttac tggtgtaata aatgaaaaag ctgcgtctcc cggagaagac gcggcctgac
     3721 agatgaaatg aatgacgttt attgtctgag aagcccttaa ctggcgagct gagtattaag
     3781 ctgtgttccg gcatcaccag cgcaactgac cttcagcatt acggataacc agccgggaat
     3841 atgttccctg gtcatcttca gtaaacacat tgcggtaagc tgttttgacg gcaacagcct
     3901 gttcgcgtga gaaagggcct tcgggcaata aacgtgatgt acaacccggc atatctgttg
     3961 ctccctgaaa gtaaaaagcc ccggtcatga tgaccggggc ctgaaggaga gtgacctgat
     4021 tatcaaaaag tcacattcag cgtggcctga ccgttataac cttcagcgct gctgccgctg
     4081 acgctgtggg cataaccggc ctgaacgccc agggtgatat tttcccggac acgggcttcc
     4141 agtccggcct gcaggtccag tgacgtgcca ttccgggacg gtgagaacgt catgttactg
     4201 ccggcggcgg ctgtccccat gctcatgtca ccccgggagc tgaaagtgcg gataacagaa
     4261 ggctgtaccc accagttcac cggcagttca ctcacacggt gttttgtact gtcgcgcaga
     4321 gtgtcacggg atgaggtgcc ttcaccaaag ctcatatcgt tgtggctgcc cagacggaaa
     4381 ccggcacgca catgttgtgc actgccatgc ccgaacttca cataaccggc gttatcctgg
     4441 ccgtcatcca gggtaagtcc ctgccaggta tagtgcagtt gtggctccag catcaggttg
     4501 tcagtgatac tgaagggcag accggtttcc agtgagccca gccagcccca gccccgggca
     4561 cggaagtcgt tattgtccga tgacgctttc atgctgtggc gggttccctg tgccacaatg
     4621 tcagcccaca ggccggagga cgtgtgtgtc agattcaggt atccgcccag gctgccggca
     4681 tcatcccgga ccgtgccggc gcgggaaccg tcatcatcct taacatcaac ggaagaatgg
     4741 cctgcggcac catacacccc tgtcgtcaga gacataccgg caacctctgt tctcagcagg
     4801 tcaccctcca gacggacgaa gccatagctg ccgctgcttt ccggcgtggc tccacgggca
     4861 ataccgccgt tgttatcgtg
//
LOCUS       DAJTNA010000243         4859 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTNA010000243 DAJTNA010000000
VERSION     DAJTNA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.248"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(178..554)
                     /gene="rnpB"
                     /locus_tag="OO161_003833"
     ncRNA           complement(178..554)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO161_003833"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(587..1732)
                     /gene="garK"
                     /locus_tag="OO161_003834"
     CDS             complement(587..1732)
                     /gene="garK"
                     /locus_tag="OO161_003834"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5464715.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFRKIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVDASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTTL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1829..2719)
                     /gene="garR"
                     /locus_tag="OO161_003835"
     CDS             complement(1829..2719)
                     /gene="garR"
                     /locus_tag="OO161_003835"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5464716.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2749..3519)
                     /gene="garL"
                     /locus_tag="OO161_003836"
     CDS             complement(2749..3519)
                     /gene="garL"
                     /locus_tag="OO161_003836"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR5464717.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3535..4833)
                     /gene="garP"
                     /locus_tag="OO161_003837"
     CDS             complement(3535..4833)
                     /gene="garP"
                     /locus_tag="OO161_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5464718.1"
                     /translation="MHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKELQLSAVSMG
                     YIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGIS
                     MFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLT
                     FAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKP
                     GSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKV
                     GLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNN
                     TTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGY
                     LVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1167 a         1410 c         1169 g         1113 t
ORIGIN      
        1 atccggcatg aataaagcgc actcaacaat ctgaaacccg ccggaacggt ttattacgta
       61 catcaggtga aactgaccga taagccgctt tcttttgggt atagtgtcgt ggacagtcat
      121 tcatctttct gcccctccaa aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg
      181 tgaaactgac cgataagccg ggttctgtcg tggacagtca ttcatctagg ccagcaatcg
      241 ctcactggct caagcagcct acccgggttc agtacgggcc gtaccttatg aacccctatt
      301 tggccttgct ccgggtggag tttaccgtgc cacggactgt taccagccgc gcggtgcgct
      361 cttaccgcac cctttcaccc ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc
      421 tctgttgcac tggtcgtggg tttccccccc aggcgttacc tggcaccctg ccctatggag
      481 cccggacttt cctcccctcc gcccgtctcc cccgaagagg acgacgacga agcggcgact
      541 gtctggtcag cttcggcgcg cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc
      601 gcattccaat cgccagtgtc gcggcgatat tacgtgaagc acggcagata ttgtcataag
      661 ccccgcggaa tgcttcgtcc aacgtaccta tgctggtcaa tacgctgaag accgcatcaa
      721 tgccatgctg atgtacaacg ccaacatcat cggtcaggct acccgcaatg ccaatcaccg
      781 gtttatggta cttcttcgcc acgtttgcga caccaatcgg taccttgccg tgaatactct
      841 ggctgtcaat acgcccttca ccggtgatca ccagcgtaca atcgtgaata tgttcttcca
      901 gattcagcgt cgtagtgacg atttcaatac cacttttcag ttccgcacca agaaacgcca
      961 tcagcgccgc gcccataccg cccgcagctc ctgcgccggg gacatctttc acatcaacat
     1021 gcagcgcttt tttaatgacc tcggcatagt gagagaggtt attgtccagc tcaacaatca
     1081 tcgcttcact ggctcccttt tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca
     1141 gcggattggt gacatcacaa gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc
     1201 cggaaatatc aatatcattc agagtattaa gactaccgcc gccaaaacca atttcattgc
     1261 cgttggcgtc gcataatttc gcccccagcg cctgtaccat gcctgcgccg ccatcatttg
     1321 tagcgctgcc gccaatgccg ataataatgt ttgtcgcacc gctctccagc gcctgcagga
     1381 ttaactcgcc tgtgccgcgt gaagtggtca cgagtggatc gcgtttttcc gcaggtacca
     1441 gctccagccc actggccgcc gccatttcaa taaacgcggt tttgccatcg ccggagatcc
     1501 cccaactggc atccactttc tcgcccaacg gccctgtaac ccaggcgtga cgttcggaac
     1561 cttgggtggc tgcaatcatc gcttccaccg ttccttcgcc accgtcggca accggaacag
     1621 aaacgtactg tgcatcagga aaaattttcc gaaatccttt ttctatcgcc tgcgcaacct
     1681 cgctggcaga taaactttct ttataagagt ctggggcgat tacgattttc atacctatgc
     1741 ctgttaccac atgacgccgg agggcgtttc tcttattcgg cgtggattcc aggcccggat
     1801 tgcaatacgc catccgggca cgacgtcatt aacgagtaac ttcgactttc gccagttttt
     1861 cgtagtagca cgccagagcg ctatgatccg ccgttcctaa accatctgca cgcagtgcct
     1921 gcatcatctc cataaccgca gctgtgagcg gcagttgcgc gccgacgccg tgagaagtat
     1981 ccagcgcatt cgccagatcc ttaatatgca gatcaatgcg gaagcccggc ttgaagttgc
     2041 ggtccatcac catcggcgct ttggcatcca gcacggtact gcccgccagc ccaccgcgaa
     2101 ttgcctgata aaccaggtcc gggttaacgc ccgctttagt tgccagcgtt aacgcttctg
     2161 acatcgcagc aatattcagc gccacaatga cctgatttgc cagtttggtg acgttacctg
     2221 caccgatttc cccggtatgc accacggaac ccgccatcgc tttcatcaaa tcatagtatt
     2281 tgtcgaaaat agccttgtcg ccgcccacca tcactgacag cgtaccgtcg atggctttcg
     2341 gttcaccgcc gctcaccgga gcatccagca tatcaatgcc tttcgctttc agcgcttcgc
     2401 tgatttcacg gcttgccagc ggtgcgatag aactcatatc gatcaatacc gtacctggct
     2461 tcgcgccttc aataatgcca ttctcaccca gcgccacctc tttcacatga ggggagtttg
     2521 gcagcatggt tatgatgacg tcgcactgtt cagcgatcgc tttagccgta gacgctgttt
     2581 ctgcacctgc agcaatcacg tcagcaatag cttctgggtt acggtcagca accaccagcg
     2641 agtaacctgc tttcagaagg tttttactca ttggtttacc cataatcccc aggccaataa
     2701 aaccaacttt catagtcata tcaatcatct ctcttgttgc ggtggtggtt attttttaaa
     2761 ggtatcagcc agtttctgag tggcagagcg gaagacgcca agatcgctgc cgacagccac
     2821 aaacgtcgcg ccccattcca gataacgacg cgcatcggct tcgaccggcg cgagaatacc
     2881 gctgggtttg ccgtgcgcac tggcacggtt aaaaatatgc tgaattgctt tttgtacatc
     2941 cggatgcgag gcattgccga gatggcccaa tgccgcagcc agatcgctgg ggccgacgaa
     3001 gataccgtct acaccttcgg tagcggcaat agcatcgacg ttatctacgc cctgctgact
     3061 ttctatctgg accagaatag tgatgttctt gttcgactga gcgaaataat ccgccacggt
     3121 gccaaacata ttggcgcggt gagaaacgga gacgccgcga atgccttccg gcgggtaacg
     3181 agttgatgcc accgcctgct ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt
     3241 atagaaaccg atatccagaa gacgcttaat aattaccggc tcgttggtcg gcactcgcac
     3301 tactggcgcg ctggcgctgc ctttcagggc cattaactgc ggaataaacg tggaaatatc
     3361 gtttggcgca tgttcgccat ccagcaccag ccagtcaaac ccagccaaac caagaacttc
     3421 agtgctaatc gggttagaga gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag
     3481 tgcggctttg aatttattcg ggaaaacatc gttattcatc gcttatacct ttgcttattt
     3541 ctgcaattcc atacgtttaa tgtcgccaac tacgaagagg tagcagacca tcgccatcag
     3601 cgctgaacat cccacgaaaa ccagtgctgc attgaaggag tgcagttcac ttaccaggta
     3661 gccaatcacc agtggagtga caatggaggc aacattgcca aagacgttaa agacgccgcc
     3721 acagaggcca acaatctctt tcggcgcggt gtcagaaatc accggccagc ccagagcacc
     3781 aaatcctttg ccaaaaaaag ccagcgccat cagcatgacc accagcgtgg tgttgttggt
     3841 gtagttacat aagatgatgg tggaagccag caacattccc agcacaatcg gtagcttacg
     3901 tgccagggtc agggataaac cgcgtttgat cagataatcc gagaagacac ctcccagcac
     3961 gccgcccgca aaaccacaca gtgccggaat cgaggcgacc agacccactt tcagaatcga
     4021 catgcctttt tcctgcacca gataaatcgg gaaccaggtg aggaagaacc aggtgatggt
     4081 gttgataaaa tattgtccga aaaatacgcc caacatcatg cggttagaga gcaattgctt
     4141 gatgtaatgc agtttgggac cgcttgctgc tgcgctgccc ggctttttgt ggtccatatc
     4201 gaccaccgcg ccattttcag agataaactt cagctcttcc gcagacatac gtgggtgatc
     4261 tgtcgggtta tgaatcaact tgatccacag cgccgtcagc acaaaaccaa tcacccccat
     4321 aacggtgaag acgtgctccc agccccaggc gaaagtcagc cagccaagca gaggcgaaaa
     4381 gagcgccagc gagaaatatt gcgccgagtt aaagatggcg gaggcagtac cacgttcttt
     4441 cgtcgggaac caggcggcga caatacgggc gttcgccggg aatgatggcg cttccgagaa
     4501 gccgagcata aagcgcataa agaacataga gatccctgcc caggccagcg ggaacatatc
     4561 aacaaagcct tgcaggaagg tgaatagcga ccagaaaaag aggctgtagg tgtaaacttt
     4621 tttcgagcca aacttatcaa gcagccagcc gccgggaatt tgcatcagca ggtaggccca
     4681 gccaaaagcg gagaagatgt aacccatcga aaccgcactt aactgcaact cttttgccac
     4741 ttcggtacca gcaatagaca gcgttgcacg atcggcgtag ttaacggcgg taacaataaa
     4801 aataatcagt aatattaaat agcgggtatg cacgcctttc ttttttacgt caacggtgt
//
LOCUS       DAJTNA010000244         4808 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTNA010000244 DAJTNA010000000
VERSION     DAJTNA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.249"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            763..4808
                     /locus_tag="OO161_003838"
                     /pseudo
     CDS             763..4808
                     /locus_tag="OO161_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
BASE COUNT         1292 a         1083 c         1298 g         1135 t
ORIGIN      
        1 tgacgggtta attatctgaa agaatttgtg aggctgtatc ggttactcat tgatttgata
       61 gttttactct cgggagagta atagatattt aatctattac tctcccgaga gtaatagata
      121 tttaatctat taacggaaac cagccagttc ctttcgatgc ctgaatttga ttccatagtt
      181 tatccggaat ggttaattca tgaataagtt gtgttctctt catgagaagc ataacgtaaa
      241 gggaaaagct cgattagacg gcaggatttg tcaggggtta tgaacgaaat tcataaatct
      301 gtttgagtgt tgcgatgagt agtgtaagtt cgatatcccc gcaatttaca gtccgatgaa
      361 ggaaaatgaa tatccataat aaatatatcg gtttatcctg gcgtatatac ctacttcgac
      421 gtatttccaa tagttgctaa ttaaaggcag gtcattatta ttcagtctga ataatgaatt
      481 attcactgtc cgcagagtaa gaaatataac ttaggtatct atttaatgac ttgcacaaaa
      541 agctaaattt tcacccataa ataaaaatat aatcccgcgc ccaaccacct gatgagtggc
      601 tacagacact ggatatatca ggtggcggtg cactttctta cataaaggta tttccttttc
      661 tgcggaaaag gaaaccggga aatccccggt ttttctgaca agcagacgcc attatttgtg
      721 tctgcctatg ttcgttaatt cgttcatcag gaaattatct caatgtcacg ttataaaaca
      781 ggtcataaac aaccacgatt tcgttattca gttctggccc gctgcgtggc gtgggcaaat
      841 atctctgttc aggttctttt tccactcgct gtcaccttta ccccagtaat ggcggcacgt
      901 gcgcagcatg cggttcagcc acggttgagc atgggaaata ctacggtaac tgctgataat
      961 aacgtggaga aaaatgtcgc gtcgtttgcc gcaaatgccg ggacattttt aagcagtcag
     1021 ccagatagcg atgcgacacg taattttatt accggaatgg ccaccgctaa agctaaccag
     1081 gaaatacagg agtggctcgg gaaatatggt acagcgcgcg tcaaactgaa tgtcgataaa
     1141 gatttctcgc tgaaggattc ttcgctggaa atgctttatc cgatttatga tacgccgaca
     1201 aatatgttgt tcactcaggg ggcaatacat cgtacagacg atcgtactca gtcaaatatt
     1261 ggttttggct ggcgtcattt ttcaggaaat gactggatga tgggggtgaa cacctttatc
     1321 gaccatgatt tatcccgtag tcatacccac attggtgttg gtgcggaata ctggcgcgat
     1381 tatctgaaac tgagcgccaa tggttatatt cgggcttctg gctggaaaaa atcgccggat
     1441 attgaggatt atcaggaacg cccggcgaat ggctgggata ttcgtgctga gggctattta
     1501 cccgcctgac cgcagcttgg cgcaagcctg atgtatgagc agtattatgg cgatgaagtt
     1561 gggctgtttg gcaaagataa acgccagaaa gatccacatg cgatttccgc tgaagtgaat
     1621 tacacgcctg tgcctcttct gaccctgagt gccgggcata agcagggcaa gagtggtgag
     1681 aatgacactc gctttggcct ggaagttaat tatcggattg gcgaacctct ggagaaacaa
     1741 ctcgatacag acagcattcg cgagcgtcga atgctggcag gcagccgcta tgacctggtt
     1801 gagcgtaata acaacatcgt tcttgagtac cgcaaatctg aagtgatccg tattgctctg
     1861 cctgagcgta ttgaaggcaa gggctgtcag acactttccc tcgggcttgt ggtcagcaaa
     1921 gcaactcacg gactgaaaaa tgtgcagtgg gaagcgccgt cattactggc tgaaggtggc
     1981 aaaattaccg gtcagggtag tcagtggcaa gtaacgctcc cggcttatcg tccaggcaaa
     2041 gacaattatt atgcgatttc tgcggttgcc tacgataaca aaggcaatac ctcaaaacgc
     2101 gtgcagacag aggtggtcat taccggagct ggtatgagcg ccgatcgcac ggcgttaacg
     2161 cttgatggtc agagccgtat tcaaatgctt gctaacggta atgaacaaaa accgctggtg
     2221 ctgtctctgc gcgacgccga gggccagcca gtcacgggca tgaaagatca gatcaagact
     2281 gaactaactt tcaaaccggc tggaaatatt gtgactcgtt ccctgaaggc cactaaatca
     2341 caggcaaagc caacactggg tgagttcact gaaactgaag ctggggtgta tcagtctgtc
     2401 ttcactaccg gaacgcaatc aggtgaggca acgattactg ttagcgttga tggcatgagc
     2461 aaaaccgtca ctgcagaatt gcgcgccacg atgatggatg tgtcgaagtc tactctgagc
     2521 gtgaatgaac tctcaggaga tgtggttgct gatggtcagc aatcccacac gctgacgctt
     2581 actgcggtgg atactgatgg taacccggtg acgggagagg ccagccgctt gcgatttgtt
     2641 ccgcaagaca ctaatggtgt caccattggt acaatttcgg agataaaacc aggcgtttac
     2701 agcgctacgg tttcttcgac ccgtgccgga aacgttgttg tgcgtgtgtg cgtgctttca
     2761 gcgagcagta tcagctgggc acattacaac aaacgctgaa gtttgttgcc gggccgcttg
     2821 atgcagcaca ttcgtccatc accctgaatc ctgataaacc ggtggttggg ggtacagtta
     2881 cggcaatctg gacggtaaaa gatgcctatg acaaccctgt gaccagcctc acgccggaag
     2941 cgccgtcatt agcgggtgcc gctgctgtag gttctacggc atcgggctgg acaaataatg
     3001 gtgatgggac gtggactgcg cagattactc tcggctctac ggcgggtgaa ttagaagtta
     3061 tgccgaagct aaatggacag gatgcggcag caaatgcggc aaaagtaacc gtggtggctg
     3121 atgcgttatc ttcaaaccag tcgaaagtct ctgtcgcaga agatcacgta aaagccagcg
     3181 aaagtacaac cgtgacgctg gtggcgaaag atgcgcatgg caacgctatc agtagtcttt
     3241 cgttgtcggc aagtttgacg gggaccgcct ctaaaggggc gaccgtttcc agttggaccg
     3301 aaaaaggtga cggttcctat gttgctacgt taactacagg cggaaagacg ggcgagcttc
     3361 gtgtcatgcc gctattcaac ggccagccag cagccaccga agccgcgcag ttgacggtca
     3421 tcgccggaga gatgtcatca gcgaactcta cgcttgttgc ggacaataag gctccgaccg
     3481 tcaaaacgac gacggaactc accttcaccg tgaaggatgc gtacaggaac ccggtcaccg
     3541 ggatgaagcc agatgcacca gtgtttagcg gtgccgccaa cacggggagt gagcgtcctt
     3601 cagcaggaaa ctggacagag aaaggtaatg gggtctacgt gtcgacctta acgctgggat
     3661 ctgccgcggg tcagttgtct gtgatgccgc gagtgaacgg ccaaaatgcc gttgctcagc
     3721 cactggtgct gaatgttgca ggtgacgcat ctaaggctga gattggtgat atgacagtga
     3781 aggttaataa ccaactggct aatggacagt ctgctaacca gataaccctg accgtcgtgg
     3841 acagctatgg taacccgttg caggggcaag aagttacgct gaatttaccg cagggtgtga
     3901 ccagcaagac ggggaataca gtaacaacca atgcggcagg taaagcggat attgagttga
     3961 tatcaaccgt tgcaggggaa cttgaaatcg cggccgcggt gaaaaactct cagaagacgg
     4021 tcacggtgaa attcaacgcg gatgccagca ccggtcaggc aaacctgcag gtagacaccg
     4081 ccgttcaaaa agtagcaaac ggcaaagatg cctttacgct gacggcgacc gttgaggata
     4141 aaaatggtaa ccctgttcca gggagcctgg tgacctttaa tctgccccgg ggtgtcaagc
     4201 cgcttacagg cgataatgtc tgggtgaaag ccaacgatga ggggaaagcc gagttgcagg
     4261 tggtttcagt gactgccgga acgtatgaga tcacggcatc ggcggggaat agccagcctt
     4321 cggatacgca gactataacg tttgtagccg ataaggctac cgcaaccgtc tccggtattg
     4381 aggtgattgg caactatgcg ctggcggacg gcaaagccaa acaaacgtat aaagttacgg
     4441 tgactgatgc caataacaat ttggtgaaag atagcgacgt gacgctgact accagcccgg
     4501 cttcgttaaa cctggaaccg aatggcactg cgacaacgaa tgagcaaggg caggctattt
     4561 tcaccgctac cactactgtt gcggcgacat acacactcaa ggcgcaagtg agtcagacca
     4621 acggtcaggt atcaacgaaa actgccgaat ctaaattcgt tgcggatgat aaaaacgcgg
     4681 tactcaccgc atcatctgat atgcaatctc tggtggcgga tgggaaatcg actgcgaagc
     4741 tggaggtgac actgatgtcg gcaaacaacc ccgttggggg gaatatgtgg gtcgacattc
     4801 agacgccg
//
LOCUS       DAJTNA010000245         4783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTNA010000245 DAJTNA010000000
VERSION     DAJTNA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.250"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..790
                     /locus_tag="OO161_003839"
     CDS             <1..790
                     /locus_tag="OO161_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HCR5464719.1"
                     /translation="NLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQ
                     GKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAAGVNATDAVN
                     VSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLK
                     QSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGT
                     YNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            1158..2813
                     /gene="lldP"
                     /locus_tag="OO161_003840"
     CDS             1158..2813
                     /gene="lldP"
                     /locus_tag="OO161_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5464720.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            2813..3589
                     /gene="lldR"
                     /locus_tag="OO161_003841"
     CDS             2813..3589
                     /gene="lldR"
                     /locus_tag="OO161_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5464721.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            3586..4776
                     /gene="lldD"
                     /locus_tag="OO161_003842"
     CDS             3586..4776
                     /gene="lldD"
                     /locus_tag="OO161_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5464722.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         1050 a         1266 c         1299 g         1168 t
ORIGIN      
        1 taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat
       61 tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga
      121 tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga
      181 caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg
      241 ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc ggtgttaatg ccaccgatgc
      301 ggttaacgtg tcgcagctga aatcttctga agcaggcggc gtgcgctacg acaccaaagc
      361 tgatggttct atcgactata gcaatatcac cctcggtggt ggcaacggca gtacgactcg
      421 tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa
      481 gcaaagcgtg caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa
      541 actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg
      601 tctgccgcag gcttatacac cgggtgccag catggcttct attggtggcg gtacttacaa
      661 cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta
      721 caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat
      781 tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc
      841 gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca atctaagggc aattctctga
      901 tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc
      961 ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca atattgccaa
     1021 tacataacat ttagttaacc attcattgtc gttatcccta cacaacacaa ttggcagtgc
     1081 cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg
     1141 actctaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc gggaatatct
     1201 ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg ctgattaagc
     1261 tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt gccgtggctt
     1321 tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat ggcttcttct
     1381 atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat aagatctcgg
     1441 tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc cctgaccagc
     1501 gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga gccgcaggct
     1561 ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt aaaccgctgt
     1621 acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc gcgatgggca
     1681 ttccaatcct ggttgccgga caagtaacag gcatcgacag ttttgagatt ggccagatgg
     1741 tggggcgtca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg gcgattatgg
     1801 acggctggcg cggtatcaaa gagacgtggc ctgcggtcgt ggttgcgggc ggctcgtttg
     1861 ccatcgctca gtaccttagc tccaacttca ttgggccgga gctgccggac attatctctt
     1921 cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcaacca gttcgcgtat
     1981 tccgcttcgg tgacttgggg gcgtcacagg ttgatatgac gctggcccac accggttaca
     2041 ccgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgaccgct accgtaacac
     2101 tgtggagcat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg tatgagtggg
     2161 tgatcaacat tccggtgccg tacctcgata aactggttgc ccgtatgcca ccagtggtca
     2221 gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc accggcaccg
     2281 ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg tctgacgcta
     2341 tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc atcggcatgg
     2401 tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg gcactggcac
     2461 tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg ctgggggtat
     2521 tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa gccaccgcag
     2581 cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc ggtggcgtca
     2641 ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta ggcctggtgg
     2701 gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc acctgtatgg
     2761 tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct taatgattgt
     2821 tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa
     2881 aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt
     2941 atcacgtaat tcactacgcg aggcgctggc aaaactagtg agcgaaggcg tgctgctcag
     3001 tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg agcaaaacat
     3061 cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg atattctgga
     3121 agcccgctac gccattgaag ccagcactgc atggcatgcg gcaatgcgcg ccacacctgg
     3181 cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc cggatatcgc
     3241 ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata acatcgtgct
     3301 gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca
     3361 gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc aggctgtcat
     3421 tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg cgcaccttag
     3481 ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg cacggattac
     3541 ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat tatttccgca
     3601 gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt ccactatatg
     3661 gatggtggtg catattctga atacacgctg cgccgcaacg tggaagattt gtcagaagtg
     3721 gcactgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac gacgctgttt
     3781 aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg catgtatgcg
     3841 cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc atggcattcc gtttactctc
     3901 tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg cccaatgtgg
     3961 ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga gcgagcaaaa
     4021 gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg cgcacgctac
     4081 cgtgatgcgc attcaggtat gagcggccca aatgcggcaa tgcgccgcta cttgcaggcg
     4141 gtgacgcatc cgcaatgggc gtgggatgta ggcctgaacg gtcgtccaca tgatttaggt
     4201 aatatctcag cttatctcgg caaaccaacc ggactggaag attacatcgg ctggctgggg
     4261 aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga tttctgggat
     4321 ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc agtacgtttt
     4381 ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc agctggacgg tgtactctct
     4441 tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc cattctggcg
     4501 gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg tgccgacacc
     4561 gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc gggtgtagct
     4621 aacctgctaa atctgatcga aaaagagatg aaagtggcga tgacgctgac tggcgcgaaa
     4681 tcgatcagtg aaattacgca agattcgctg gtgcaagggc tgggtaaaga gttgcctgcg
     4741 gcactggctc ccatggcgaa agggaatgcg gcatagtcgt ttg
//
LOCUS       DAJTNA010000246         4705 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTNA010000246 DAJTNA010000000
VERSION     DAJTNA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.251"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..407)
                     /gene="dsbG"
                     /locus_tag="OO161_003843"
     CDS             complement(<1..407)
                     /gene="dsbG"
                     /locus_tag="OO161_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5464723.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREIWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQA"
     gene            779..1342
                     /gene="ahpC"
                     /locus_tag="OO161_003844"
     CDS             779..1342
                     /gene="ahpC"
                     /locus_tag="OO161_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5464724.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1513..3078
                     /gene="ahpF"
                     /locus_tag="OO161_003845"
     CDS             1513..3078
                     /gene="ahpF"
                     /locus_tag="OO161_003845"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5464725.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3199..3627)
                     /gene="uspG"
                     /locus_tag="OO161_003846"
     CDS             complement(3199..3627)
                     /gene="uspG"
                     /locus_tag="OO161_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5464726.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            3848..>4705
                     /locus_tag="OO161_003847"
     CDS             3848..>4705
                     /locus_tag="OO161_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR5464727.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEVA"
BASE COUNT         1217 a         1134 c         1199 g         1155 t
ORIGIN      
        1 gcctgctgcc agaactgttt acaatatggg cagaacggat cggcgaagac gtagacaatg
       61 accggcgcat cttttttacc gtcgaggagc cagtgggatt gttccatccg ttgccatatt
      121 tcgcgtccgg ctggtgcgta aatttctttt tcgataagtg tgttactcag gttttcacct
      181 ttctcgttgt acatgtaacc agagatagcg tgcttaccat ctggagtcag gtagatggtg
      241 acgcccatat cctgatactt tccgagataa cctttcattc ctccgggggc atcgaacgtt
      301 ttgatgattg taatgccctg tttttcaatc gcttttactg gagcaggaag ttcctctgcg
      361 aaggcgattg caggaagcag agccagtaaa agtatctttt ttaacattaa tttgtccttt
      421 tcagtcagtg caaaagtcga gtaaaaggca taacctatca ctgtcatagg taagagctta
      481 gatcaggtga ttgccctttg tttatgaggg tgttgtaatc catgtcgttg ttgcatttgt
      541 aagggcaaca cctcagcctg caggcaggcg ctgaagatac caaagggtag ttcagattac
      601 acggtcacct ggaaaggggg ccattttact ttttatcgcc gctggcggtg caaagttcac
      661 aaagttgtct tacgaaggtt gtaaggtaaa acttatcgat ttgataatgg aaacgcatta
      721 gccgaatcgg caaaaattgg ttaccttaca tctcatcgaa aacacggagg aagtatagat
      781 gtccttgatt aacaccaaaa ttaaaccttt taaaaaccag gcattcaaaa acggcgaatt
      841 catcgaaatc accgaaaaag ataccgaagg ccgctggagc gtcttcttct tctacccggc
      901 tgactttact ttcgtatgcc cgaccgaact gggtgacgtt gctgaccact acgaagaact
      961 gcagaaactg ggcgtagacg tatacgcagt atctaccgat actcacttca cccacaaagc
     1021 atggcacagc agctctgaaa ccatcgctaa aatcaaatat gcgatgatcg gcgacccgac
     1081 tggcgccctg acccgtaact tcgacaacat gcgtgaagat gaaggtctgg ctgaccgtgc
     1141 gaccttcgtt gttgacccgc agggtatcat ccaggcaatc gaagttaccg ctgaaggcat
     1201 tggccgtgac gcgtctgacc tgctgcgtaa aatcaaagca gcacagtacg tagcttctca
     1261 cccaggtgaa gtttgcccgg ctaaatggaa agaaggtgaa gcaactctgg ctccgtctct
     1321 ggacctggtt ggtaaaatct aaatttcctt cgtctttcgc gctaccaact ccggcgcgaa
     1381 atacttcgga aattcaccta attcttcggg tgctgcggca cccgattttc ttccccgcac
     1441 tatgatgcaa gctgcatcca ggtagccgta gaggccgctt gcatgatgat gtttaaagcc
     1501 caggagataa acatgctcga cacaaatatg aaaactcaac tcaaggctta ccttgagaaa
     1561 ttgaccaagc ctgttgagtt aattgccacg ctggatgaca gcgctaaatc ggcagaaatc
     1621 aaggaactgt tggctgaaat cgcagaactg tcagacaaag tcacctttaa agaagataac
     1681 agcttgccgg tgcgtaagcc gtctttcctg atcaccaacc caggttccaa ccaggggcca
     1741 cgttttgcag gctccccgct gggccacgag ttcacctcgc tggtactggc gttgctgtgg
     1801 accggtggtc atccgtcgaa agaagcgcag tctctgctgg agcagattcg ccatattgac
     1861 ggtgattttg aattcgaaac ctattactcg ctctcttgcc ataactgccc ggacgtggtg
     1921 caggcgctga acctgatgag cgtactgaac ccgcgcatca agcacactgc aattgacggc
     1981 ggcaccttcc agaacgaaat caccgatcgc aacgtgatgg gcgttccggc agtgttcgta
     2041 aacgggaaag agtttggtca gggccgcatg acgttgactg aaatcgttgc caaaattgat
     2101 acgggcgcgg aaaaacgtgc ggcagaagag ctgaacaagc gtgatgctta tgacgtatta
     2161 atcgtcggtt ccggcccggc gggtgcagcg gcagcaattt actccgcacg taaaggcatc
     2221 cgtaccggtc tgatgggcga acgttttggt ggtcagatcc tcgataccgt tgatatcgaa
     2281 aactacattt ctgtaccgaa gactgaaggg cagaagctgg caggcgcact gaaagttcac
     2341 gttgatgaat acgacgttga tgtgatcgac agccagagcg ccagcaaact gatcccagca
     2401 gcagttgaag gtggtctgca tcagattgaa acagcttctg gcgcggtact gaaagcacgc
     2461 agcattatcg tggcgaccgg tgcaaaatgg cgcaacatga acgttccggg cgaagatcag
     2521 tatcgcacca aaggcgtgac ctactgcccg cactgcgacg gcccgctgtt taaaggtaaa
     2581 cgcgtagcgg ttatcggcgg cggtaactcc ggcgtggaag cggcaattga cctggcgggt
     2641 atcgttgagc acgtaacgct gctggaattt gcgccagaaa tgaaagccga ccaggttctg
     2701 caggacaaac tgcgcagcct gaaaaacgtc gacattattc tgaatgcgca aaccacggaa
     2761 gtgaaaggcg acggcagcaa agtcgttggt ctggaatatc gagatcgtgt cagcggcgat
     2821 attcacaaca tcgaactggc cggtattttc gtgcagattg gtctgctgcc gaacaccaac
     2881 tggctcgaag gcgcagtcga acgtaaccgc atgggtgaga ttatcattga tgcgaaatgc
     2941 gaaaccaacg tcaaaggcgt attcgcagcg ggtgactgta cgacggttcc gtacaagcag
     3001 atcatcatcg ccactggcga aggtgccaaa gcctctctga gtgcttttga ctacctgatt
     3061 cgcaccaaaa ctgcataaga agaagtaaga ttcacctgca attgcttagc cgccggggtc
     3121 aaacctagcg gcttttttat ggcattaaaa agccctgccg ggatgttcca ggcagggcgg
     3181 cataaatact tttatctgtt agcgaacgac cagtacaggc agattggcgt ggcggatcac
     3241 gctcgaggcg ttagaaccta acagatgggt cgaaatcgat gggttgcgag aaccgataac
     3301 tacaacatca gcccccagtt cttctgccaa ctcattgact tcatcccgca cgctaccaaa
     3361 acggacatgt tgtttaatgc gggaaggatc gatggtgaag tggctgacca tcgtttgcag
     3421 acgttcttct gcttcatgtt gcagatgctc ttcaaaacga cgcacatcag cggcaaaacg
     3481 gtgcaggctc aggctggctg acccgggtag tacgtgaagt agatgaataa ctccgtcatc
     3541 ctgggtgagg aattcagcgt ggcgaacagc tttgtcgctc aattccattt caaatacatc
     3601 aactggcata atgattgtct tatacataac cctttctccc tgttaatcat gaacaaatca
     3661 ttcgccatga ttataatatt tatccctgat atttgtctgg ttctttttcc ttacgaacgg
     3721 tttctgtgat taatatattc tcactgaaca ccaggaattc tcccaaaacc tgcggtaccg
     3781 cccgttttcc cgctgtgata gctaccctta aagactgact cttttttgaa ctgtctctgg
     3841 aggttgcatg aaagcattga cttatcacgg cccacatcac gttcaggtag aaaatgttcc
     3901 cgatccgggc attgaacagg cagatgatat tattctgcgt attacggcaa cggcgatctg
     3961 tggctctgat ctccatcttt atcgaggcaa aataccccag gttaaacatg gcgatatttt
     4021 tggtcatgaa tttatggggg aagtcgttga aaccggaaag gacgtaaaaa atttgcaaaa
     4081 aggcgaccgg gtggtaattc cgtttgtcat tgcttgtggc gactgttttt tctgtcgaat
     4141 gcagcaatat gccgcctgcg aaaataccaa tgcgggtaaa ggcgctgcgc tcaataaaaa
     4201 acagatacca gctcccgcgg cattgtttgg ttatagtcac ctgtatggtg gcgttcctgg
     4261 tggacaggcg gaatatgtcc gcgtccctaa agggaatgtg gggccgttta aagtaccgcc
     4321 tttgctttca gatgataaag cccttttcct ttctgatatt ctgccaacgg catggcaggc
     4381 agcaaaaaat gcgcagatcc aacaaggttc aagcgttgca gtctatggtg ccggtccagt
     4441 gggattgttg acaatcgcct gtgcacggtt gctcggtgcg gaacagattt ttgttgttga
     4501 tcatcatccc taccgcttga gtttcgccgc tgaccgctac ggcgcgatcc cgattaattt
     4561 cgatgaagat agcgatccgg cacagtcaat tattgaacaa acggcaggtc accggggcgt
     4621 ggatgcagta atagacgccg tcggttttga agcgaaaggc agcaccacgg aaacggtgct
     4681 gactaatctg aaacttgagg tagcc
//
LOCUS       DAJTNA010000247         4701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTNA010000247 DAJTNA010000000
VERSION     DAJTNA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.252"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..2312
                     /locus_tag="OO161_003848"
                     /pseudo
     CDS             1..2312
                     /locus_tag="OO161_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
     gene            2332..3084
                     /locus_tag="OO161_003849"
     CDS             2332..3084
                     /locus_tag="OO161_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5464728.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREILPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQADEEQSRKG"
     gene            3100..3609
                     /locus_tag="OO161_003850"
     CDS             3100..3609
                     /locus_tag="OO161_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5464729.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            3606..4094
                     /locus_tag="OO161_003851"
     CDS             3606..4094
                     /locus_tag="OO161_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5464730.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            4091..4630
                     /locus_tag="OO161_003852"
     CDS             4091..4630
                     /locus_tag="OO161_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5464731.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGAQAVDNNLHFYGNLQSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVSGQSAHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
BASE COUNT         1172 a         1203 c         1310 g         1016 t
ORIGIN      
        1 gtggcgaaaa atctgcaatg gagccacgat gctaaatgcc tgaaatccgg tcaactggaa
       61 ggcatggaaa ttaaggctga tttaagccag tccgcattag tcatttcatt gccacaggct
      121 tacctcgaat atacttatcc cgactgggat ccgccttcac gttgggatga cggcatctcc
      181 gggatcgtcg cggactacag catcaacgca caaacccggc acgaagaaaa tggcggtgat
      241 gatagtaacg agatcagcgg caacgggacg gtcggggtta acctggggcc gtggcgtatg
      301 cgtgctgact ggcagactaa ctatcaacat actcgcagta atgatgacga tgaattcagc
      361 ggcgatgaaa ctcaaaaaaa atgggagtgg agtcgctact atgcctggcg ggcgttacca
      421 tcattaaaag ccaaactggc gctgggcgag gattacctca gatccgatat ttttgatggt
      481 tttaactatg ttggtggcag tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc
      541 ggctacgcgc cagacatttc cggcgtggca cacaccacag caaaagtgac cgtcagccag
      601 atggggcgtg tgatttacga aacgcaggtt ccggctggac cgtttcgtat tcaggatctt
      661 ggtgattctg tctccggtac gttgcatatt cgcattgaag aacagaacgg ccaggtgcag
      721 gaatatgaca tcagcaccgc ctcgatgcca tacctcactc gtccaggtca gattcgctat
      781 aagatcatga tgggccgtcc gcaagagtgg ggacaccatg tcgagggtga attttttctg
      841 gtgctgaagc ttcctggggg atcgctaacg gctggtcgtt atatggcggc gcactgggag
      901 atgaaaacta tcagtctgcg gcgcttggcg tcggtcgcga tttgtctaca ttcggcgcgg
      961 tcgcgtttga tgttactcac tcgcacacca aactggataa agacaccgct tatggcaaag
     1021 gttcgctgga cggtaactcc ttccgtgtga gttattccaa agactttgac cagctcaaca
     1081 gccgcgttac ttttgctgga tatcgcttct cggaagagaa ctttatgacc atgagcgagt
     1141 atctggatgc cagtgacagc ggaatggtac gcacgggcaa cgacaaagag atgtacaccg
     1201 ccacttataa ccagaacttc cgcgatgcgg gtgtttcggt ttatctcaac tatacccgcc
     1261 atacctactg ggatcgcgag gagcagataa actacaacat catgctctcg cactatttca
     1321 atatgggcag tattcgtaat gtcagcatct cgatgactgg ctaccgttac gagtatgaca
     1381 accaggccga caaaggcatg tacatttcgc tcagtatgcc gtggggcgac aacagtaccg
     1441 ttagctataa cggtaactat ggcagtggga cggacagcag tcaggtcggt tatttcagcc
     1501 gtgtcgatga cgcgactcac tatcagttga acgtcggcac cagtgacaaa cacaccagcg
     1561 ttgacggctg ttacagccat gatggttcgc tggcgcaggt tgacctcagt gcgaactacc
     1621 atgaagggca atacacctct gcgggcttgt cgttacaggg cggcgcaacg cttactgccc
     1681 acggtggcgc acttcaccgt acccagaata tgggcgggac acgcttgttg attgatgccg
     1741 atggcgttgc cgatgttccg gtggaaggta acggggctgc tgtttatacc aatatgtttg
     1801 gtaaagccgt cgtttctgac gtcaataact attaccgcaa tcaggcgtat atcgacctca
     1861 acagattgcc tgaaaacgct gaagcaaccc agtcggtggt gcaagccacg ctaactgaag
     1921 gtgccattgg ctaccgtaaa tttgctgtca ttagcggaca aaaagcgatg gcggtgctgc
     1981 gcctgagcga cggcagccat cctccgtttg gcgcagaagt aaaaaatgat aacgagcaga
     2041 cagtgggcct tgtcgatgat gacggcaatg tttatctggc tggggtgaaa cctggcgaac
     2101 acatgagtgt gttctggagt ggtgttgcgc attgcgatat caacctgccg gacccgctgc
     2161 ctgccgatct gtttaacggc ttgttactgc catgccagca taaaggcaat gtagcaccta
     2221 tcacttcgcc ggcggtcaaa ccggcgattc aggaacagac acagcgggtg acgccaacgg
     2281 aaccaccgac ttcaatttca gtaaaccagt aacgtgatta aggaatgata catgtttaat
     2341 ctgactaaca ctgcaaaaat cgttgtcccg gcactggcgc tgctggcgac agcggtcagt
     2401 ttctccagcc acgcttcggt gacgcccgac aggacacggc tggtgtttaa cgaaagcgac
     2461 aagtccatca gcgtcacgct gcgcaacaac gatcccacac tgccttatct ggcgcagagc
     2521 tggatagagg atgcaaaggg caacaaaatc agctcgccgc ttgcggtgct tccgccagtg
     2581 cagcgcatcg atgcgatgat gaacggccag gtgaaggtgc aggggctgcc ggatatcaac
     2641 aaactgccgg ccgaccgcga gagcgtgttc tatttcaacg tgcgcgaaat tctgccgaaa
     2701 tcaaataaac cgaacacact gcagattgcg ctgcagacgc gtatcaaact gttctggcga
     2761 ccgaaggcgc tggagaacac cagcatgaaa agtccgtggc agcataaggt gacgctcatc
     2821 cgtaacggcc aggacttcac cgtcaataac ccgacgccgt actacgtgat catcagcaac
     2881 gccagcgcta aaaaaggcgg caacccgccg gcgggattca caccgctgat gatggagccg
     2941 aaaacctccg caccgctgaa tgcgaagatg gacagcgtgc cggtgctgac ttacatcaac
     3001 gactacggcg cgcggatgcc gctgtacttc aaatgcaccg gcaacagctg ccaggcggat
     3061 gaagagcaga gccgcaaagg ctgatgccgg gagggactta tgagtcacaa gtttgtagcg
     3121 ggcgcagggt tgctggtgct ggccggatgg ggcatcagcg gtttggctgt tgctggcagt
     3181 gatatcaaac aggtggatct gacgctgcga atcctggtcg atggaccgcc gccttgctcc
     3241 gtgaagggaa gcgctgtgaa atttggcaat gtgataatca acaggatcga tggtaccaat
     3301 taccaacagg atgcgaaata caccctcaac tgcggtaata aagtcagcga tgacttacgg
     3361 atgcagctca aaggcaatac cgccgtgatc aacggagaaa cggttctcag cacggatatc
     3421 gccggcctgg gggtgcggat agaaaacgcc gccgacaact ccctgtttgc agttggtgaa
     3481 aataactgga cgccttttaa tgtcaacact cagcctcagc tgaaagcggt gccggtaaaa
     3541 cagagcgggg tgcaactgac gccgtcggag tttaacgcca cgatgaccat ggtggtggat
     3601 taccaatgaa aaaattcctg ctccttttac tgtgcgctgg ttgcggggct gtgctggcgg
     3661 cggaagatga catcacattc cacggtacgc tgatgtcgcc gcccgcctgt agcatcagcg
     3721 gcgggcaaac catcgaagtg caatttcgcg atctcattat cgacagcatt gatggcaatt
     3781 atggtcgtca gaaagtggac tacgaactga gctgcgactc ggatgtccgc gatcccgagt
     3841 ggaatatgac actctcctgg acggggactg ccacctccta caacgactct gctattgaga
     3901 cggatgtacc gggcttcgga attgagcttc agcatgacgg tcagcgtttt aagctcaacg
     3961 agccgctttc cattaacgcc accgatttta gccagaagcc aaagctggag gcggtaccgg
     4021 tcaaagcggc tgatgcggtg ctgacggaca cgaccttttc tgcttacgca accttacggg
     4081 tggattacca atgaaaacag cacacattta ttcacccggt tatgcgctgt tagcaggcct
     4141 gctgctgacc gccagcacag gagcacaggc agtcgacaac aacctgcatt tttacggcaa
     4201 cctgcagagt aaatcctgca cgctggtggt ggatggtgcc aacctggcag aaatccattt
     4261 tcccaccgtc agcaaccggg atttgatggt atctgggcag tccgcgcatc tgccggttgt
     4321 gtttaagctg aaagactgca aagggccggc gggatacaac gtcaaagtca cgctgagcgg
     4381 caccgaagac agcggtcagc cgggttttct ggcagtcgat gacacctcca ccgcgcaggg
     4441 cgtggggata gggatggaaa cgctcgacgg caagcgggtg gatattaaca acaccaccgg
     4501 ggcgaccttt gcgcttacca acggcaacaa tgatattaat ttccgggcct ggatccaggc
     4561 gaaaagcggt cgtgatgtaa ccatcggtga tttcaccgcc agcctgacag caacttttga
     4621 gtatatttaa aatgaaaatc cttcgtcatg ttttatgtct tttattgctg ttaatgttca
     4681 atacatgcgg ccaggcggcg a
//
LOCUS       DAJTNA010000248         4700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTNA010000248 DAJTNA010000000
VERSION     DAJTNA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.253"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(89..2353)
                     /gene="maeB"
                     /locus_tag="OO161_003853"
     CDS             complement(89..2353)
                     /gene="maeB"
                     /locus_tag="OO161_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5464732.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEA"
     gene            2642..3592
                     /gene="tal"
                     /locus_tag="OO161_003854"
     CDS             2642..3592
                     /gene="tal"
                     /locus_tag="OO161_003854"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5464733.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3612..>4700
                     /locus_tag="OO161_003855"
     CDS             3612..>4700
                     /locus_tag="OO161_003855"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5464734.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYVNELQANPAKIATRKASQNT"
BASE COUNT         1158 a         1253 c         1196 g         1093 t
ORIGIN      
        1 aagctttact aatatattgc agccaaaccg cttaccctga atattcaggg taagcgtgag
       61 agttaaaaaa gaattacagc ggttgggttt acgcttcaac cacggccagc gccaccatgt
      121 tgacgatacg acgcaccgat gcgatcggcg ttaacacgtg aaccggtttc gccacgccca
      181 tcagcaccgg accgacagtc acaccttccg agctggaaac gcgcagtaag ttgtaactaa
      241 tgcgggcagc ttccatgttc ggcatcacca gaatattggc agaacctttc aaagggctgt
      301 ccggcatacg gtcgttgcga atcgcttcca ccagcgctgc atcgccgtgc atttcaccat
      361 caatcatcag atctggcgca cgatccctga ccagttccag cgcctgacgc attttgctcg
      421 acgacgggca gtcagaagaa ccaaagttgg agtgcgacaa cagagcaacg cgcggctcaa
      481 taccaaaacg acggacagtt tctgccgcca tcaaggtgat ctccgccagc tcttctgcgt
      541 ctggttcgtc attgacgtag gtatcggcaa taaaggtgtt accactcggc agcagcagcg
      601 cgttcattgc acctgcggtg tgaacgccat cgcgataacc aaagacattt ttcaccacgc
      661 taaaatgttc atgataatcg cccaccgtac cgcagatcat tgcatcggct tccccacgct
      721 gaaccatgat cgcgccgatc actgtcgggt tactgatcag cgcccgctgc gcctgttcct
      781 gagtgacacc gcgacgcttc atgatctgga agtattcggt ccagtactct ttaaagcgcg
      841 gatcggattc gttattgacg atctcaaaat caacgcccgc tttgatctgc aagcccagtt
      901 tctgaatgcg catttcgatc acgttcggac gaccgataag gatcggtttc gccagtccca
      961 gcgttaccag ttcctgagtg gcatgcagaa cgcgcgcctc ttccccttct ggcagaacaa
     1021 cgcgcttcgg cgctttgcga gcctgggaga aaatcggctt cataaacagg ttggttttgt
     1081 aaacgaactc agtcagcttg tcgatgtaga cgtcgaaatc agcaatcgga cgcgtcgcca
     1141 cgcccgactc catcgcggct ttagcgaccg caggagcgat cttaacgatc aagcgcggat
     1201 caaacggttt tggaatgatg tattccggac caaagctcag atcctgatcg ccatacgctg
     1261 aagccaccac ttcgctctgt tccgcatggg cgagttctgc aatcgcacgt accgccgcca
     1321 gtttcatctc ttcgttgatg gcggttgcgc caacgtccag cgcgccacgg aagatgaacg
     1381 ggaagcacag gacgttgttc acctggttcg gatagtcaga acgaccggtg caaatgatgg
     1441 catccggacg cacttctttc gccagcggcg gcagaatttc cggttccggg ttcgccagcg
     1501 ccaggatcat tggcgcacga gccattttct tcaccatttc ctgggtcagc actttcgggc
     1561 cggaacatcc gaggaaaata tccgcgcctt caatcacatc atcgagggta cgtttgccgt
     1621 catccaccac cgcatatgcg gctttggttt ccgccatgtt tggctcacgg ccctgataga
     1681 taacgccttt tgaatcgcaa accacgatgt tatgtttttg cagacccagc gctaccagca
     1741 ggttcataca ggcgattgct gcggcacccg cgccggaaac caccatccgc acgtcggaga
     1801 tgtttttctc caccacgcgc aagccgttga ggatggcggc agtgctgatg attgccgtgc
     1861 cgtgctgatc gtcgtggaat accggaatat tcatccgctc gcgcagtttc tgttcaatat
     1921 agaaacattc tggcgcttta atgtcttcga ggttgattcc gccgaaggtc ggttcgagcg
     1981 cagcgacaac ttcaataaat ttgtccgggt cgagttcatc aacttcaatg tcaaatacat
     2041 caatcccggc gaatttctta aacagaacgc ccttgccttc catcaccggt ttgccagcca
     2101 gcgcgccaat gttgcctaac cccagcaccg ccgtaccgtt agagatcacc gccaccaggt
     2161 taccgcgcgc ggtatatttg taggctttta acgggtcttt ttcgatttca agacaaggtg
     2221 cggcaacgcc tggtgagtag gccagcgcca gatcgcgctg cgttgccaga ggcttggttg
     2281 gagaaacctg gattttccct ggaactggaa attcatggaa atcaagtgca ctttgtttta
     2341 actggtcatc catttggttg ttcctttcac gtaacgttca caaataaagt gtgtgggcaa
     2401 cagcccctgc ccacaacgtg gcgcacatta ttaccccgcc ggagtctaca gactttgagc
     2461 aagtccaaac tctcaccatt aatataatgt tttggtaata atcctataac actgatgtta
     2521 cctgcttaat ccagcaatac catgcctgtc tgctatgctt ttttgatgcg tttagcgaaa
     2581 tttctcagaa gtgtgaatta acgcactcat ctaacacttt acttttcaag gagtatttcc
     2641 tatgaacgag ttagacggca tcaaacagtt caccactgtc gtggcagaca gcggcgatat
     2701 tgagtccatt cgccattatc atccccagga tgccaccacc aatccttcgc tgttgctcaa
     2761 ggctgccgga ttatcacaat atgagcattt aatagacgat gctatcgcct ggggtaaaaa
     2821 aaatggcaag acccaggaac aacaggtggt cgcagcgtgt gacaaactgg cggttaattt
     2881 cggtgctgaa attctcaaaa tcgtacccgg tcgcgtgtca acagaagttg atgcacgcct
     2941 ctcttttgat aaagaaaaga gtattgagaa ggcgcgccat ctggtggact tgtatcagca
     3001 acaaggcgtt gagaaatcac gcattctgat caaactggct tcgacctggg aaggaattag
     3061 cgcggcagaa gagctggaaa aagaaggtat taactgcaac ctgacgctgc tgttttcttt
     3121 tgcccaggcg cgggcctgtg cggaagcagg cgtttttctg atttcgccgt ttgtcgggcg
     3181 tatttatgac tggtatcagg cacgcaagcc gatggacccg tatgtggtgg aagaagatcc
     3241 gggcgttaaa tcggtgcgca atatctacga ctactataag caacaccact atgaaaccat
     3301 tgtgatgggc gcgagcttcc gtcgcaccga acaaatcctc gccttaaccg gctgcgatcg
     3361 actgactatc gcaccgaatt tgctgaagga gctgcaggaa aaagtttcgc cagtggtacg
     3421 taaattaatc ccaccttctc agacgttccc acgcccagct cccatgagcg aagcggagtt
     3481 ccgttgggag cacaatcagg atgcgatggc ggtagaaaaa ctgtctgaag gcattcgtct
     3541 gttcgccgtt gatcaacgca aactggaaga tcttcttgcc gccaaactat aaaccagcca
     3601 cggagtgtta tatgtcccga aaagaccttg ccaatgcgat tcgcgcactc agtatggatg
     3661 cggtacaaaa agccaactct ggtcatcccg gcgcgccgat gggcatggct gatattgccg
     3721 aagtgctgtg gaacgatttt cttaaacata accctaccga cccaacctgg tatgatcgcg
     3781 accgctttat tctttccaac ggtcacgcgt cgatgctgct ctacagtttg ctgcatctga
     3841 ccggttacga cctgccgctg gaagagctga agaacttccg tcagttgcat tcgaaaaccc
     3901 caggccaccc ggagattggt tatacgcccg gtgttgaaac caccaccggt ccacttggac
     3961 aaggtttggc gaacgccgtc ggactggcga tagcagagcg tacactggcg gcgcagttta
     4021 atcagccgga tcatgaaatt gtcgatcact tcacctatgt gtttatgggc gacggctgcc
     4081 tgatggaagg tatttcccac gaagtctgtt cgctggcggg cacgctagga ctgggcaagc
     4141 tgattggttt ttacgatcac aacggtattt cgattgatgg agaaaccgaa ggctggttta
     4201 ccgacgatac ggcaaaacgt tttgaagcct atcactggca tgtgatccat gaaatcgacg
     4261 gtcacgatcc gcaggcggtg aaggaagcga tccttgaagc gcaaagcgtg aaagataagc
     4321 cgtcgctgat tatctgccgt acggtgattg gctttggttc gccgaataaa gcaggtaagg
     4381 aagaggcgca cggcgcacca ctgggggaag aagaagtggc gctggcacgg caaaaactgg
     4441 gctggcacca tccgccattt gagatcccta aagagattta tcacgcctgg gatgcccgtg
     4501 aaaaaggcga aaaagcgcag cagagctgga atgagaagtt tgccgcctat aaaaaggctc
     4561 atccgcaact ggcagaagag tttacccgac ggatgagcgg tggtttaccg aaggactggg
     4621 agaaaacgac tcagaaatat gtcaatgagt tacaggcaaa tccggcgaaa atcgctaccc
     4681 gtaaggcttc gcaaaatacg
//
LOCUS       DAJTNA010000249         4669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTNA010000249 DAJTNA010000000
VERSION     DAJTNA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.254"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..756)
                     /locus_tag="OO161_003856"
     CDS             complement(<1..756)
                     /locus_tag="OO161_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR5464735.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLQQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLS"
     gene            complement(887..2251)
                     /gene="sdaA"
                     /locus_tag="OO161_003857"
     CDS             complement(887..2251)
                     /gene="sdaA"
                     /locus_tag="OO161_003857"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5464736.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPKQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(2435..3013)
                     /locus_tag="OO161_003858"
     CDS             complement(2435..3013)
                     /locus_tag="OO161_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5464737.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(3017..4378)
                     /gene="pabB"
                     /locus_tag="OO161_003859"
     CDS             complement(3017..4378)
                     /gene="pabB"
                     /locus_tag="OO161_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5464738.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVTKILRQ
                     LEK"
     gene            4452..4631
                     /locus_tag="OO161_003860"
     CDS             4452..4631
                     /locus_tag="OO161_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5464739.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         1173 a         1200 c         1214 g         1082 t
ORIGIN      
        1 acttagcagc accgccagcg gtaattgtgt tggaagatag tgccatgcca gcgccgtagc
       61 ccccggacca ttaacgttaa tggtaaaagg aaagcgttgc gaagaaacct gatagttttc
      121 attgtcttca ggttgcggaa gggaatctac cagcccatta ccatagagca aatgccgttt
      181 gtccaccgtc aggctggcgc tactgatttg ctgcagttgt ggctcaagga gcattgccgt
      241 cagtaagtcg atgttaatca tctccatgac cccagcattg ctactgcccg ctgcaggcgt
      301 ccattgaatc aaaaccggac tgcctttaat cagggcacgg tcgatcgtca ggcgtaaaag
      361 tggttgcggt gcaggaagtt cagccagaat gtccacgacg ggcacattgc gataaccaaa
      421 aatgctggag caatagagtg tgccgtcttg caccaggcca atggatcgca cagtttggag
      481 ttttgccgcc tgtttacgta atggcaaatg ggcgacagag cagggcagac caatcagcgg
      541 aagcaagact tcgctacctg cctgtagggg aagcagtact ttatctaatt cctctacagc
      601 gtgattggcg aattgtacta cccgttgttg atttaagtta cgctgtgaaa taaatcgcac
      661 gctcagggtc gaagcgagcg taacgagtgc gcaaatcgta caaacaatca ttcgattacg
      721 tcgataggtt ttaatgatcc gttgtgcttt ttgcatgcgg gccaccctgt aagcgtatgg
      781 cgacaaacgc ctgacgcaac agtggatgtg tagtggggaa tattacagca gacgagaaag
      841 cgggtataaa ttcgcccatc cgttgcagat gggcgagtaa gaagtattag tcacactgga
      901 ctttgattgc cagaccaccg cgtgaggttt cgcggtattt ggcgttcatg tccttaccgg
      961 tttcgtacat cgtttcgatg accttatcca gcgagacgcg cggtgcactg gtgcggcgca
     1021 gagccatccg tgcggcgtta atcgccttca cagaggcaat ggcattacgc tcaatgcacg
     1081 gcacctgaac ctgccctgca accgggtcgc aggttaaacc aaggttgtgt tccatgccaa
     1141 tttccgccgc cacgcaaacc tgtttcgggc taccgcccag cagttctgca agacccgcag
     1201 cagccattga acaggcaaca cccacttcgc cctggcaacc aacttccgca ccggaaatag
     1261 aggcgttcat tttatacaat gcaccaatcg cgcccgctgc cataaagtaa cgggtataga
     1321 tgtccgggct gaccgattca ataaagtggt catagtaagc cagcactgcc ggaacgatac
     1381 cgcaggcacc gttggttggc gcagttacca cacgaccacc ggcggcgttt tcttcgttaa
     1441 ctgccagcgc aaacatgttt acccagtcaa tgacattcat cggatcgtta gacagtttat
     1501 cgctggaaac cagcatccgg cgcagggcag acgcacgacg tggcacgcgc agcgggcctg
     1561 gcagtacacc ttcggtgttc atcccgcgat cgatacatgc ctgcatggtt tgccagacat
     1621 gcgcgaaata ctcgtcgatc tctttcttgc tgtgcagcgc cagttcgttc tgcatagcga
     1681 gaccagacag cgaatagccg gtttcattac agtacgcgag cagttcggtg gcagatttga
     1741 acggatacgg cacgctcact tcgttggcag catcctgacc aaagtgttct tcatcgacga
     1801 taaaaccgcc gccgatggaa taataagttt tgctgtagac gacttcatcg ccgttatagg
     1861 cgtggatttg cataccgttt tcatgcagcg gcaggttgcc gttatgaaaa cgcatcccgt
     1921 tgtcgcgagg gaaatccact tcatgccgtc cctgtgccag cagcagacgt tcgcgctctt
     1981 ctacgtcgcg aataaaaccg ggaatactgt cgatatccac ggtggcaggt tcgttacctg
     2041 caagacccat aataatggcg atatcggtgt ggtggccttt acccgtcagc gacagtgaac
     2101 cataaacgtc cacggcaacg cgagtaacgc tatccagtaa gcctttttcg accagatcat
     2161 cgacgaactg tttacccgcc ttcataggcc ctacggtatg ggaagatgag ggaccaatcc
     2221 ccaccttaaa catgtcgaat agactaatca cgataatact cctgacaagg tgactggact
     2281 tccagtaacg aactaataac agcgcatagt gtaagaggga acgcggcgac tggcttaact
     2341 attcacatga attaaactaa tcaaaaaccg cgatttaact aatattgcga tgattgtctc
     2401 aagtagtgtt ttgtagatgt aaagataagt atagtcaggg tttcacacca atttgcagcg
     2461 ccagctcacg aattatgcct gcggtcattc cccatacaaa atactgttcg taccaggaca
     2521 gccatacccg atgtgaatcg ccacggcggt agatatctaa agggtgataa cgacccagat
     2581 gtaatgcctg ggcgagcggc atttcaaaca ccgccgagac ttcatcttca ctggcgcgat
     2641 acggcagatc gggcgggata atgccgacca ctggggttac ctgatagcca gttacgctat
     2701 cgacgggcgg cagcacgccg ataacttcaa cggcggaagg cggtatagcg acctcttctt
     2761 cagcttcgcg cagcgcggcg gcgataactg atgcgtccgt gtcatcgact gcacctccag
     2821 ggaatgccac ttgtccagcg tgtttacgca gatgaattga acgctgagtc agcaacaacc
     2881 ccggttgcgg tcgacggacg atggggatta acacggcagc ctgacgatga tttagggttt
     2941 cccggttaat ttgcgggcgc aaaagttgaa agcgagataa aaaatcatca agcgtcaggc
     3001 tacggtattc cacgtcttac ttctccagtt ggcgtaatat cttagtaact ttatcaaaag
     3061 tttcctgata ttccgcttct tcctggctat cggcgacaat tccaccgccc gcagagcagt
     3121 atatttgtcc gttaatggca gtcagcgtgc ggatagtaat gctggtatcc atgttgccgc
     3181 aaaagctcaa atagccaatg ctgccgcacc aggcattacg tcgctgcggt tccagttcgt
     3241 cgataatttc catagcccgt actttcggag ccccggttat tgagccacca ggaaaagctg
     3301 cgcgcagcag atcgctggcg tgtaactgtt ctggtagtcg cgccgttata gtgctgacca
     3361 gatgatgcac ggcagggaag ggttccacca cgaagagctc tggtactttt accgaaccgg
     3421 ctacggcaac acgaccgata tcattacgca ttaaatcgac aatcatcaga ttttcggcac
     3481 gatctttcgc tgagttcgcc agtttttctg cttgtttgct atcttcctga ggatcgggca
     3541 ggcgtggtag cgtgccttta atcgggcggg tctggatttc actattatca caaagaataa
     3601 accgctctgg cgaaaggctt aaaattgcac cctgttcaag acgtaaaaaa gcgctaaatg
     3661 gcgcgcggtt ggcctgatta agctgaagga atgcctgcca ttcatcgcca gaataggtcg
     3721 catgaaaacg ctgggcgaga ttcacctgat agcaatcacc gctgtgcaga tattcctgta
     3781 cctggcgaaa tttttcgccg tactgctcgc gggtcatatt ggattgccag tcggaagtga
     3841 gcgtgaaatc ttcctgcggc gagaattgct ggctttccag ccaggcccga cgggcattga
     3901 catcattatg actcagcaaa gaaactgtat gacgctggtg gtcgacaatg agcgcccaat
     3961 cgtagatacc cactgccata tccggcagaa cgatatcttg ttccgcaatt tctggcagtg
     4021 actcaaaacg gcggcccaga tcgtagccaa acaaccccag tgcgccgccc tgaaatggca
     4081 aatcttcgtt atgcgttggg cgaatgtctg cgcgatccag cacctgctgg agcacctgta
     4141 gcgggtcatc agtggtcgtt gtgcgttttt cgctttcact aacaacggtt tctttaccga
     4201 aagtggttaa agtgcaaatc ggctcggcga ccacaatatc aaagcggcta tacggatgat
     4261 cggcatagcc ggagtgtaaa agcatcgccc acggcaggtg gcttaagcgg gagaaataaa
     4321 attcagcggc gtcctgacgc cagggtaaag taatcacagc gggagataac gtcttcatca
     4381 gtcctgactc tactggctat gtgccgggta gcatgaaata attagcgcca acaatttagc
     4441 aggagttaac aatgtttgca ggtttacctt cactcaccca tgaacagcag caaaaagctg
     4501 tcgagcggat ccaggaactg atggcacaag ggatgagcag cggccaggca atcgcgctgg
     4561 tggcagaaga gctgcgtgcc aaccactccg gcgagcgaat cgtggcgcgt tttgaggatg
     4621 aagacgagta acacactaat ccgtatcgat ttcgttatag aagccgatg
//
LOCUS       DAJTNA010000250         4655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTNA010000250 DAJTNA010000000
VERSION     DAJTNA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.255"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            308..643
                     /locus_tag="OO161_003861"
     CDS             308..643
                     /locus_tag="OO161_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5464740.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            803..1246
                     /gene="yjdN"
                     /locus_tag="OO161_003862"
     CDS             803..1246
                     /gene="yjdN"
                     /locus_tag="OO161_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5464741.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSYIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEIAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1379..2167
                     /gene="phnC"
                     /locus_tag="OO161_003863"
     CDS             1379..2167
                     /gene="phnC"
                     /locus_tag="OO161_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5464742.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPITSLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2192..3208
                     /gene="phnD"
                     /locus_tag="OO161_003864"
     CDS             2192..3208
                     /gene="phnD"
                     /locus_tag="OO161_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5464743.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWMPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRT
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3334..4113
                     /gene="phnE"
                     /locus_tag="OO161_003865"
     CDS             3334..4113
                     /gene="phnE"
                     /locus_tag="OO161_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR5464744.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4134..>4655
                     /gene="phnF"
                     /locus_tag="OO161_003866"
     CDS             4134..>4655
                     /gene="phnF"
                     /locus_tag="OO161_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR5464745.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQ"
BASE COUNT         1125 a         1338 c         1196 g          996 t
ORIGIN      
        1 aattttatat ttagtgtgtt gttttttatc agaataaata acgtcttctg atgcgtttaa
       61 aacgtcagaa agataaaaat atcatgtgaa taaaaaaaga acaagtagag cattaacatt
      121 atcttaaata ataaatagag gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga
      181 aaacgatcgt cgtctaaaat cagcagtacc cccgacaaac tcagggattt tgtgtataat
      241 tgcggccttt ttcggcaatc tgccgttttt tggcgctttt gccctgctga cttttgagga
      301 aatccacatg tcattaccac actgcccaaa atgcaactcc gaatacactt acgaagataa
      361 cggcatgtac atctgcccgg aatgtgccta cgaatggaac gacgcagaac ctgcacagga
      421 aagcgacgag ctgatcgtta aagatgctaa cggcaatctg ctggctgacg gcgacagcgt
      481 taccatcatt aaagatctga aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa
      541 agtgaaaaac atccgcctgg ttgaaggcga ccataacatc gattgcaaaa tcgacggttt
      601 tggtccgatg aaactgaaat ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta
      661 agccggataa ggcgctcgcg ccacatccgg caacgatgcc gactgcctga tgcgacgctg
      721 accgcgcctt atcaggcttc cctctcatct gtataaattt cgaactacac ttaactggct
      781 tctcttaact gaggtcacca tcatgccgtt aagtccctac ctctcttttg ccggtaactg
      841 ttccgacgcg attgcctatt atcaacgtac gttgggcgcg gaactgctct ataaaatcag
      901 cttcggcgaa atgccaaaat cagcgcagga cagcgccgag aactgccctt ccggaatgca
      961 atttcccgat accgccatcg cccacgccaa cgtgcgcatt gccggaagct acatcatgat
     1021 gagcgatgct ataccgtcag gaaaagccag ctactccggc tttacgctgg tgctcgattc
     1081 gcaacaggtc gaagaaggaa aacgctggtt tgataacctt gccgctaacg gaaaaattga
     1141 aatagcctgg caggaaactt tctgggcgca tggcttcggc aaagtcaccg ataaatttgg
     1201 cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg caataacccg ccgggaggcc
     1261 cgccctcccg cactgtcatc gaattcccgt taactcttca tctgttagtc acttttaatt
     1321 aaccaaatcg tcacaataat ccgccacgat ggagccactt ttttagggag gctgcatcat
     1381 gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc aatcagcatc aagcgctgca
     1441 tgcggttgat ctgaacattc atcacagtga aatggtggct ctgcttgggc cgtcgggttc
     1501 cggcaaatcc acacttttac gtcacttaag cggtttgatt accggcgata aatccgccgg
     1561 cagccatatc gagctgctgg gccgcacagt ccagcgcgaa ggccgtctgg cgcgcgatat
     1621 ccgcaaaagc cgcgcccata ccggctacat cttccaacaa ttcaacctgg tgaatcgcct
     1681 gagcgtactg gagaacgtgc tgattggcgc gctcggcagc acgccgttct ggcgcacctg
     1741 ttttagctgg ttcacccgcg agcagaaaca acgcgcgtta caggcgctga cccgcgttgg
     1801 catggtgcat tttgcccatc aacgcgtttc caccctctcc ggcggacagc agcagcgtgt
     1861 ggcgattgcc cgcgcgctga tgcagcaggc gaaggtgatt ctggccgatg aacccatcac
     1921 ctcgctggac ccggaatccg cccgcatcgt gatggacacc ctgcgcgaca tcaatcagaa
     1981 cgacggcatc accgtagtcg tcacgctgca tcaggtggat tacgccctgc gctactgcga
     2041 acgcatcgtc gccctgcgcc aggggcacgt tttctacgac ggcagcagcc aacagtttga
     2101 taacgaacgt tttgaccatc tctaccgcag cattaatcgc atcgaagaga acgcgaaagc
     2161 tgcctgacat ccccatcatt gaggaaaacg aatgaacgct aagataattg cctcgctggc
     2221 cttcaccagc atgttcagcc tcagcaccct gttaagcccg gcacacgccg aagagcagga
     2281 aaaggcgctg aatttcggca ttatttcaac ggaatcacag caaaacctga aaccgcaatg
     2341 gatgccattc ttacaggata tggagaagaa gctgggcgtg aaggtgaacg ccttctttgc
     2401 cccagactac gcaggcatta tccagggaat gcgcttcaat aaagtggata tcgcctggta
     2461 cggcaacctg tcggcaatgg aagcggtgga tcgcaccaac ggccaggtct tcgcccagac
     2521 ggtcgcggcg gatggatcgc caggttactg gagcgtgttg atcgtcaaca aagatagtcc
     2581 gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat ctcaccttcg gcaatggcga
     2641 tcctaactcc acctctggct tcctagtccc cggttactac gtctttgcca aaaacaatat
     2701 ctccgccagc gactttaagc gcaccgtcaa cgctgggcat gaaaccaacg cgctggccgt
     2761 cgccaacaag caggtggatg ttgccaccaa caacaccgaa aacctcgaca agctgaaaac
     2821 ctccgcgcca gagaagctga aagaactgaa ggtgatctgg aagtcgccgc tgatcccagg
     2881 cgatccgatc gtctggcgca agaatctctc cgaaaccacc aaagacaaga tctacgactt
     2941 ctttatgaac tacggaaaaa cgccggaaga gaaagcggtg ctggaacgcc tgggctgggc
     3001 cccgttccgc gcctccagcg acctgcaact ggtgccgatt cgccagctcg cactgtttaa
     3061 agagatgcag ggcgtaaaaa gcaataaagg actgaatgag caggacaagc tggcgaaaac
     3121 caccgagatt caggcgcagc tggatgacct ggaccgcctg aacaacgcgt taagcgcgat
     3181 gagttcggtg agtaaagcgg tgcagtaatg tgctcgatcg gtcccctctc ccttgaggga
     3241 gagggttagg gtgaggggga aatgcgcacg ctgatgccct catcccggcc ctctcccaca
     3301 gggagaggga gaaaacatta aacggagcca aacatgcaaa ccatcaccat cgccccaccc
     3361 aagcgtagct ggttctcgct tctgagctgg gccgttgttc tcgccgtgct ggtcgtctcg
     3421 tggcagggcg cggaaatggc cccgcttacg ctgattaaag acggcggcaa catggcaacc
     3481 ttcgttgccg acttcttccc gcccgatttc agccagtggc aggattacct caccgaaatg
     3541 gccgtcacgc tgcaaatcgc cgtctggggc accgcgctgg cagtggttct ctccatcccc
     3601 ttcggtctga tgagcgccga aaacctggtg ccgtggtggg tttaccagcc cgttcgccgc
     3661 ctgatggatg cctgccgcgc cattaacgaa atggtcttcg ccatgctgtt cgtggtcgcc
     3721 gtcggtctcg gcccgttcgc tggcgtgctg gcgctattta tccacaccac cggcgtgctc
     3781 tccaagctgc tttccgaagc ggtggaagcg atcgaacccg gcccggtaga aggcattcgc
     3841 gccaccggcg ccaacaagct ggaagagatc ctttacggcg tgctgccaca ggtaatgccg
     3901 ctgctgatct cctactccct ctatcgcttc gaatccaacg tccgctcggc gaccgtcgtc
     3961 ggcatggtcg gtgcaggcgg gatcggcgtc accctgtggg aagcgattcg cggttttcag
     4021 ttccaacaaa cctgcgccct gatggtgctt atcatcgtca cggtcagcct gctggatttc
     4081 ctctctcaac ggttgcgtaa gcactttatc tgataagcga ggcattgata tctatgcact
     4141 tgtctacaca tccgaccagc tacccaacac gctatcagga gatagccgca aaacttgagc
     4201 aggagcttcg tcaacactat cgctgcggcg actatcttcc cgccgagcag cagctggcgg
     4261 cgcgctttga ggtaaatcgc cacaccctgc gccgcgccat cgaccaactg gtggaaaaag
     4321 gctgggtaca gcgccgtcag ggcgtcggcg tgctggtgct gatgcgcccg ttcgattacc
     4381 cgctcaacgc ccaggcgcgt tttagccaga acctgctgga tcagggcagc catcccacca
     4441 gcgaaaaact gctgtcggtg ctgcgcccgg catctggtca cgtcgctgac gcgctgggga
     4501 taaccgaggg ggagaacgtc atccacctgc gcaccctgcg ccgggtcaac ggcatcgcgc
     4561 tctgtttaat cgaccactac tttgcggacc tcaccctttg gccgacgctg caacgcttcg
     4621 acagcggctc gctgcacgat tttctgcgcg agcaa
//
LOCUS       DAJTNA010000251         4636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTNA010000251 DAJTNA010000000
VERSION     DAJTNA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.256"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            413..1663
                     /gene="frc"
                     /locus_tag="OO161_003867"
     CDS             413..1663
                     /gene="frc"
                     /locus_tag="OO161_003867"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5464746.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            1717..3411
                     /gene="oxc"
                     /locus_tag="OO161_003868"
     CDS             1717..3411
                     /gene="oxc"
                     /locus_tag="OO161_003868"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5464747.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3481..4425
                     /gene="yfdV"
                     /locus_tag="OO161_003869"
     CDS             3481..4425
                     /gene="yfdV"
                     /locus_tag="OO161_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5464748.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            4499..>4636
                     /locus_tag="OO161_003870"
     CDS             4499..>4636
                     /locus_tag="OO161_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HCR5464749.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GH"
BASE COUNT         1228 a          985 c         1087 g         1336 t
ORIGIN      
        1 gctcgcggtt ctataaacac gcattttatc ttcctcaaag taaaccccaa taatcccgtg
       61 tcatcacaaa tcatttatta tgttatgttt tatggtacgc tttaaaaagc gtaatcatac
      121 ccttacttgt aaatcaccgg cgcttcttac agttgtaaga ataacatcac aaatgtcgcg
      181 actctctctg attaccacga atcaatattt atcatattct tcttttcgtc ctcactactt
      241 accatgcaga aaggaattgg aagtggcgat gaaatgattt tcagcacttg atacaggatt
      301 ttaaaaatat cgagtgcagc tacgacgata ttttgcgggt tatttataga agatattacg
      361 ccttaaataa acgctaaatt aacacatatt taattaacta ataggtattg ctatgtcaac
      421 tccacttcaa ggaattaaag ttctcgattt caccggtgtg caatctggcc catcttgtac
      481 tcaaatgctg gcctggtttg gcgctgacgt tattaaaatt gaacgtcccg gcgttggtga
      541 cgtaacgcgt caccagctgc gagatattcc tgatatcgat gcgctttact tcaccatgct
      601 taacagtaac aaacgttcta ttgagttaaa taccaaaaca gcggaaggca aagaggtaat
      661 ggaaaagctg atccgcgaag ctgatatctt agtcgagaac tttcatccag gggccattga
      721 tcacatgggc ttcacctggg agcatattca agaaatcaat ccacgtctga tttttggttc
      781 gatcaaaggg tttgatgagt gttcgcctta tgtgaatgta aaagcctatg aaaacgttgc
      841 tcaggcagcg ggtggcgcgg catccactac gggtttttgg gatggtccgc cgctggtaag
      901 cgctgcagcg ttgggtgaca gcaacaccgg aatgcatttg ctgatcggtt tacttgctgc
      961 tttgctgcat cgcgaaaaaa cggggcgtgg gcaacgagtc actatgtcaa tgcaggatgc
     1021 cgtattgaac ctttgccgcg tgaaattacg tgaccagcag cgtctcgata aattgggtta
     1081 tctggaagaa tacccgcagt atccgaatgg tacatttggt gatgcagttc cccgcggtgg
     1141 taatgcaggt ggtggcggtc agcctggctg gatcctgaaa tgtaaaggct gggaaaccga
     1201 tcctaacgcc tatatttatt tcactattca ggagcaaaac tgggaaaaca cctgtaaagc
     1261 catcggcaaa ccagaatgga ttaccgatcc ggcatacagt acagcccatg cccgacagcc
     1321 acatattttc gatatttttg ctgaaatcga aaaatacact gtcactattg ataaacatga
     1381 agcggtggcc tatttgactc agtttgatat tccttgtgca ccggttttaa gtatgaaaga
     1441 aatttcactt gatccctctt tgcgccaaag tggcagtgtt gttgaagtgg aacaaccgtt
     1501 gcgtggaaaa tatctgaccg ttggttgtcc aatgaaattc tctgctttta cgccggatat
     1561 taaagctgcg ccgctattag gtgaacatac cgctgctgta ttgcaggagc taggttatag
     1621 cgacgatgaa attgctgcaa tgaagcaaaa ccacgcaatc tgatattaac gggggcttct
     1681 ggctcccaat ttataaaaat ttcgaggtta ttaatcatgt cagatcaact tcaaatgaca
     1741 gatggtatgc atatcatcgt tgaagcatta aaacagaata atattgacac tatttatggt
     1801 gttgtaggta ttcctgtgac ggatatggca cgccatgccc aggcggaagg cattcgttat
     1861 attggttttc gtcatgagca gtcggcaggc tatgccgctg cggcaagcgg ttttcttacc
     1921 caaaaaccgg ggatctgcct gacagtttct gcgccaggat tcctcaatgg tttgacggca
     1981 ttggccaacg caacggtaaa tggttttccg atgatcatga ttagcggctc cagcgaccgc
     2041 gcgatcgtcg acctacagca aggtgattat gaagagctgg accaaatgaa tgcggcaaaa
     2101 ccgtatgcca aagcagcatt tcgcgttaat cagccgcagg atcttggcat tgcattggca
     2161 cgcgctatcc gggtctctgt atcgggtcgc cctggcggag tttatcttga tttgccagca
     2221 aatgtcctgg ccgcgacgat ggaaaaagac gaagcgttaa ccacgattgt taaagttgaa
     2281 aatccgtcgc cagcattatt gccatgcccg aagtcagtca ctagcgcaat ttcgctttta
     2341 gcaaaagctg aacggccatt aattatcctt ggcaaaggcg cggcgtattc acaagctgat
     2401 gaacagcttc gtgaatttat tgaaagtgct cagattccat tcctgccaat gtctatggcg
     2461 aaagggatcc ttgaagatac gcatccactt tctgcggcag ctgcgcgttc gtttgccctg
     2521 gcaaatgctg acgttgtcat gcttgttggt gcacgactga attggttatt ggcacacggt
     2581 aaaaaaggat gggcggcaga tacacagttt attcaactgg atattgaacc gcaggaaatt
     2641 gacagcaacc gccccattgc tgtgccagtc attggtgata ttgcatccag tatgcaaggt
     2701 atgctggcag aactgaaaca aaacacattt acgactccac tggtatggcg cgatatttta
     2761 aatatccaca agcagcaaaa tgcacaaaaa atgcatgaaa aattaagtac agatactcaa
     2821 ccattaaatt actttaatgc attaagtgct gtgcgcgacg tattgcgcga gaaccaggat
     2881 atttatttag ttaatgaagg tgcaaatacc ctggataatg cacgaaatat tattgatatg
     2941 tataaaccac gtcgtcgtct ggattgtggt acctggggtg tcatgggcat cggtatgggc
     3001 tatgccatcg gtgctagcgt gacttctggt tctccggttg tcgccattga aggtgatagt
     3061 gcttttggtt tcagtgggat ggaaattgaa acgatttgtc gatataacct gccggtgacg
     3121 atcgttattt ttaataatgg cggcatctac agaggagacg gtgttgatct cagtggcgct
     3181 ggtgcaccat caccaacgga tctgttgcac catgcaaggt atgacaaatt aatggatgcg
     3241 tttcgtggcg ttggctataa cgtcaccacg acagatgaac ttcgtcatgc tttaaccacc
     3301 ggtattcagt cgcgcaaacc gaccattatt aatgtggtca tcgaccctgc agcaggaact
     3361 gaaagtggcc atattaccaa acttaaccca aaacaagtcg ctggtaatta aatactgaca
     3421 tcgtctcctc ttgccaactc attatgcgat ggtgagggga cccttatcgg gaatactatt
     3481 atgctaacat tttttattgg cgatttattg cctattatcg taatcatgct gttgggttat
     3541 tttagcggca gacgagaaac tttttcagaa gatcaagccc gcgcatttaa taaactggta
     3601 ttaaactacg cgcttcctgc ggctctattt gtatctatta ctcgggcaaa cagggaaatg
     3661 atttttgcgg acactcgtct gacccttgta tcacttgtgg ttattgtcgg atgtttcttt
     3721 ttctcctggt tcggttgcta caaatttttt aaacgtaccc atgcagaagc agctgtatgt
     3781 gcattaattg caggttcacc taccattgga ttcctggggt ttgcagttct cgatcctatt
     3841 tatggtgatt ccgtatcaac aggtttagtg gtagcaatta tttctattat tgttaacgca
     3901 attactattc ctattggtct gtatttgctg aatccttctt caggagcgga tggtaagaag
     3961 aatagtaatc tgagtgcatt aatttctgcg gcaaaggagc cagtagtatg ggcacctgtt
     4021 ctggcaacga tcctggtgtt ggttggggta aaaattccgg cagcatggga cccaaccttt
     4081 aatctgattg cgaaggctaa ctcaggggta gcggtattcg ctgcggggtt gactctggct
     4141 gcacataaat tcgagttcag tgccgaaatt gcttataaca ccttcctgaa gctgattctg
     4201 atgccactgg cactgcttct cgttggtatg gcatgtcatt tgaacagcga acatctgcag
     4261 atgatggtac tggcaggcgc attaccgccg gcattctccg gaattattat tgccagccgg
     4321 tttaatgtct acacccgtac tggtacagcg tcattggctg tgagcgtact gggctttgtc
     4381 gtcacggctc ccttgtggat ttatgtcagt cgactggttt cataattaat catgttattg
     4441 attttacata aatgccgaag tgtcttttca ccttcggctt ttttcaaggc ataagcaaat
     4501 gacaaataat gaaagcaaag ggccgtttga aggcttatta gttatcgata tgacacatgt
     4561 ccttaatgga cctttcggaa ctcaacttct ttgtaatatg ggcgcaaggg taattaaagt
     4621 tgagccgccg ggtcat
//
LOCUS       DAJTNA010000252         4622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTNA010000252 DAJTNA010000000
VERSION     DAJTNA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.257"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..678
                     /locus_tag="OO161_003871"
     CDS             <1..678
                     /locus_tag="OO161_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5464750.1"
                     /translation="DAKISQFDLNAIEAASQLATDDDEIAALTVGGSLLQNSKVRKDV
                     LSRGPHSLYLVQDAQLEHALPLDTAKALAAAIEKIGFDLLIFGEGSGDLYAQQVGLLV
                     GEILQLPVINAVSAIQRQGNTLVIERTLEDDVEVIELSVPAVLCVTSDINVPRIPSMK
                     AILGAGKKPVNQWQASDIDWSQSAPLAELVGIRVPPQTERKHIIIDNDSPEAIAELAE
                     HLKKALN"
     gene            698..1636
                     /locus_tag="OO161_003872"
     CDS             698..1636
                     /locus_tag="OO161_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5464751.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1692..2981
                     /locus_tag="OO161_003873"
     CDS             1692..2981
                     /locus_tag="OO161_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5464752.1"
                     /translation="MSDDKFDAIVVGADVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKIFFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGRFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            2978..3271
                     /locus_tag="OO161_003874"
     CDS             2978..3271
                     /locus_tag="OO161_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5464753.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            3328..>4622
                     /gene="fadK"
                     /locus_tag="OO161_003875"
     CDS             3328..>4622
                     /gene="fadK"
                     /locus_tag="OO161_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5464754.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVRAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYCSGDLCRMDEAGYIKITGR"
BASE COUNT         1103 a         1178 c         1266 g         1075 t
ORIGIN      
        1 gacgccaaaa tcagccagtt cgatctcaat gccattgaag ctgcaagcca gctcgcaaca
       61 gatgacgatg agatagccgc gctgaccgtt ggcggctcat tgttgcagaa ctcgaaagtg
      121 cgcaaagatg tgctatcccg cgggccgcac agcctgtatt tggtgcagga tgcgcaactt
      181 gaacatgcac tgcctctcga taccgcaaaa gcgctggcgg cagcaattga aaagatcggc
      241 ttcgatttac tgatctttgg tgaaggttcc ggcgaccttt atgcccagca ggttggcttg
      301 ctggtcggag aaattctgca acttccggtg attaatgcag tgagtgctat tcagcgtcag
      361 ggcaatacac tggtgattga acgcacgctt gaagatgatg ttgaagttat tgaactctct
      421 gttccagccg tgctctgcgt cacctccgat attaacgtgc cacgcattcc ttcgatgaaa
      481 gccattctcg gcgcgggtaa aaaaccggta aatcagtggc aggcaagtga tattgactgg
      541 agccagagcg cgccacttgc tgaactggtc ggcattcgcg taccgccgca aacagaacgt
      601 aagcacatca ttatcgataa cgattcgccg gaggccattg ccgagctggc ggaacatctg
      661 aagaaagccc tgaactgaag cctacggaga agaaacgatg agtcaattaa acagcgtctg
      721 ggtctttagc gataatcctg aacgttatgc tgaactgttt ggcggcgctc agcaatgggg
      781 ccaacaggtg tatgccattg tacaaaatac cgaccaggcg caggcagtta tgccttatgg
      841 tccaaaatgt atttatgttc ttgagcaaaa cgacgcgctg caacgcactg aaaattacgc
      901 cgaaagcatt gctgccccgc tgaaagataa acaccccgct atgttgctgt tggccgcgac
      961 gaaacgtggt aaagctctgg cagcacggtt aagtgtgcaa ctgaatgcgg cgctggtgaa
     1021 cgatgccacg gcggtggata ttgtcgatgg tcacatttgc gccgaacacc ggatgtatgg
     1081 cgggttagcg ttcgctcagg aaaagatcaa cagcccgctg gcgatcatta cccttgcacc
     1141 cggtgttcag gaaccgtgca ccagtgatac ctctcatcag tgcccgacag aaacggtacc
     1201 ttatgttgct ccgcgtcatg aaattctctg tcgcgaacgc cgtgcgaaag ccgcaagcag
     1261 cgtggacctg agcaaagcaa aacgtgtggt tggcgtcggt cgtggtctgg cggcgcagga
     1321 tgacctgaaa atggtccacg aactggcggc ggtgctgaat gctgaagtcg gctgttcacg
     1381 tccaattgcc gaaggcgaga actggatgga gcgtgaacgt tatatcggtg tctccggcgt
     1441 gttgctgaaa tccgatctct acctgacgct ggggatctcc gggcagatcc agcatatggt
     1501 tggcggcaac ggcgcaaaag tgattgtcgc catcaataaa gataaaaatg cgccaatctt
     1561 caattatgcc gactacggtc tggtgggcga tatctacaaa gtcgtccctg ccctgattag
     1621 ccagttgagc cgctaattcc cctttcaacc acatctcccg ctgtaaacgc agcgggaagg
     1681 gagcataacg aatgtcggat gacaaatttg atgccattgt ggtcggtgcg gacgttgctg
     1741 gtagcgttgc cgcactggtc atggcgcgag ccggactgga tgtcctggtg atagaacgcg
     1801 gcgacagtgc cggatgtaaa aacatgaccg gcgggcgtct ttatgcccac acacttgaag
     1861 caatcattcc aggctttgca gtatcagcgc cggtagaacg caaggtcaca cgcgagaaaa
     1921 tcttcttctt aaccgaagaa agcgccgtta ccctcgattt tcaccgcgag caaccagatg
     1981 ttccgcaaca cgcatcttat accgtattgc gtaatcgtct ggacccgtgg ttgatggaac
     2041 aagccgagca ggcgggcgca cagtttatcc cgggcgttcg cgtcgatgcg ttggttcgtg
     2101 aaggaaacaa ggtcactggc gtgcaggccg gggatgatat tctccaagcg aatgtggtgg
     2161 ttctagctga tggcgtaaac tcgatgcttg gccgctcgct gggaatggtt cccgcttccg
     2221 atccgcatca ttacgctgtt ggtgttaaag aggttattgg cctcacacca gaacagatca
     2281 acgatcgctt taatattacg ggcgaggaag gtgccgcctg gctgtttgcc ggttcccctt
     2341 ctgacggcct gatgggcggg agatttctct ataccaacaa ggattccata tccttggggc
     2401 tggtttgtgg attgggtgat atcgcccatg cgcaaaaaag cgtgccgcaa atgctggaag
     2461 attttaaaca acaccccgcc attcgcccac taattagcgg cggcaaactg cttgaatatt
     2521 ccgcgcatat ggtgccagaa ggcggtctgg caatggtgcc gcagatggtt aacgatggcg
     2581 tgatgatcgt tggtgacgcc gcaggcttct gcctgaattt gggttttaca gttcgtggca
     2641 tggatttagc cattgcatcg gctcaggctg ccgccacaac ggtgatcgcc gccaaagaac
     2701 gcgaggattt ctccgccagc agtctggcgc aatacaaacg tgagctggaa caaagctgcg
     2761 tcatgcgcga tatgcagcat ttccgcaaga tcccggcgct gatggaaaac ccgcgcctgt
     2821 ttagccaata cccacgaatg gtcgccgaca tcatgaacga gatgttcacc attgacggca
     2881 aaccaaacca gccggtacgc aaaatgatca tgggacacgc gaagaaaatt gggctgatca
     2941 acttgctgaa agatggcatt aagggagcaa ccgcgctatg agccagaacg ctacggttaa
     3001 cgtcgacatc aaattaggcg tcaataaatt ccatgttgat gagggccacc cgcatatcat
     3061 tctggcggca aatcccgata tcaatgaatt ccgtaaatta atgaaagctt gccctgccgg
     3121 actttataag caggatgacg ccggcaacat tcatttcgat tccgccggtt gtctggagtg
     3181 cggcacctgt cgggtgctgt gcggcaacac tattctcgaa cagtggcaat atcccgcagg
     3241 caccttcggt attgattttc gctacggcta atcatgcatc ccacaggccc gcatctcggg
     3301 cctgatgttc tgtttcgaga gtccaaaatg aaagtgacat taacgttcaa cgaacaacgt
     3361 cgtgcggcgt atcgtcagca agggttatgg ggcgatgctt cactggccga ttactggcag
     3421 cagaccgctc gtgcgatgcc agacaaaatt gccgtggtcg acaatcatgg tgcaacgtac
     3481 acctatagcg cgctcgatca cgccgcgagc tgtctggcaa actggatgtt agcgaagggt
     3541 attgaatcag gcgatcgcat cgcatttcaa ctgcctggct ggtgtgaatt taccgttatc
     3601 tatcttgcct gcctgaaaat cggtgcggtt tccgtgccgc tgttgccttc ctggcgggaa
     3661 gcagaactgg tgtcggtgct caataagtgt caggcaaaaa tgttctttgc accgacgttg
     3721 tttaaacaaa cacgtccggt agatttaatc ctgccgctgc aaaatcagct tccacaacta
     3781 caacaaattg tcggcgtgga caaactggct cccgccacct cttccctctc attaagtccg
     3841 attatcgccg acaatacctc actgaccacg gcgataacga cccacggcga tgaattagct
     3901 gcggtgctgt ttacctccgg aactgagggt ctgccaaagg gcgtgatgct aacgcataac
     3961 aatattctcg ccagtgagcg ggcttattgc gcgcgactga atctgacctg gcaggatgtc
     4021 tttatgatgc ctgcgccact tggtcacgca acgggctttc tgcatggcgt aagggcacca
     4081 ttcttaattg gcgctcgcag cgtgttgtta gatattttca ctcctgatgc gtgtctcgcg
     4141 ctgcttgagc agcagcgttg cacctgtatg ctaggcgcaa cgccgtttgt ctatgatctt
     4201 ttgaatgtac tagagaaaca acccgcggac ctttcagcgc tgcgtttctt tctttgcggc
     4261 ggtaccacaa tccccaaaaa agtggcacgt gaatgccagc agcgcggcat taaattatta
     4321 agtgtttatg gttccacaga aagttcgccg catgcggtgg tgaatctcga tgatcctttg
     4381 tcgcgcttta tgcacaccga tggttacgct gccgcaggtg tagagattaa agtggtcgat
     4441 gacgcacgca agaccttacc gccaggttgc gaaggtgaag aagcctcgcg tggccccaat
     4501 gtgtttatgg ggtattttga tgaacctgaa ttaaccgccc gtgcgctgga cgaagaaggc
     4561 tggtattgca gcggcgatct ctgccgtatg gatgaggctg gctatataaa aattaccgga
     4621 cg
//
LOCUS       DAJTNA010000253         4605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTNA010000253 DAJTNA010000000
VERSION     DAJTNA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.258"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..1841)
                     /locus_tag="OO161_003876"
     CDS             complement(<1..1841)
                     /locus_tag="OO161_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5464755.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGKACAFNDIIPADHCLHDVQDMSALNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATYAQQPDLFTAMLKQFRTDLSGFNAQCHGGSAAVV
                     PWICGDTTYYWKNTYGTQYDSVYGAYKNRESDNVFFVPFMTDGNGNNTPTNLPAEDPD
                     IADAGYYGAQSRSNGNWVSSNRPTHFSSWARRGIISDRLATAILNAVGRTSAFISGTA
                     PEIKPSPGGDTPSGPSDGDTSVRTVSLLPTAGEAAAQGWTITGGSVALEDGVFKVTKQ
                     SNKTWSLMHPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMT
                     GTGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQTLELVFTAGSATVTP
                     KLNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINA"
     gene            2101..2436
                     /gene="iraM"
                     /locus_tag="OO161_003877"
     CDS             2101..2436
                     /gene="iraM"
                     /locus_tag="OO161_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5464756.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            2507..2719
                     /locus_tag="OO161_003878"
     CDS             2507..2719
                     /locus_tag="OO161_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464757.1"
                     /translation="MPLHGAGCLPVNSVPAPESAIIPYTYSLIAPPHRGIHHAKFFNK
                     LPAGQTIIASLNYEQRSILRENCFLQ"
     gene            complement(3185..3295)
                     /locus_tag="OO161_003879"
     CDS             complement(3185..3295)
                     /locus_tag="OO161_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR5464758.1"
                     /translation="MVDFSSRIMSVLSDDVLVAGVVVVAFPLLKKKASGD"
     gene            complement(3385..3461)
                     /locus_tag="OO161_003880"
     tRNA            complement(3385..3461)
                     /locus_tag="OO161_003880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3425..3427),aa:Arg,seq:tcg)
     gene            complement(3469..3544)
                     /locus_tag="OO161_003881"
     tRNA            complement(3469..3544)
                     /locus_tag="OO161_003881"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3509..3511),aa:Ile,seq:cat)
     gene            complement(3585..>4605)
                     /locus_tag="OO161_003882"
     CDS             complement(3585..>4605)
                     /locus_tag="OO161_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR5464759.1"
                     /translation="NADCLEFIWSLPENSVDLIVTDPPYFKVKPEGWDNQWKGDDDYL
                     KWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNHIIWAKPSGRWNGC
                     NKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMAPLISYFRDARAAL
                     GITAKQIADATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVAEEKHQRGELEKPH
                     HQLVDTYTSLNRQYVELQSEYKHLRRYFGVTAQVPYTDVWTHKPVQFYPGKHPCEKPA
                     EMLQQIISASSRPGDLVADFFMDSGSTVKAALALGRRAIGVELETGRFEQTVREVQDL
                     VSQNG"
BASE COUNT         1150 a         1281 c         1066 g         1108 t
ORIGIN      
        1 gcatttatct ccagcaccag actttctatc tcaacgccat acgctgcatt tttggtaata
       61 tccgtcagcg tcagcgcatt cagccccagt gtcagactgt cttttatgac ctggaatgcc
      121 gggccagcca ctccattcag tttcggagta accgtggcac tgccggcggt gaacaccagc
      181 tccagcgtct gccagtcgtt actgtaattc ccgaactcgc ccaactttgt gtttccggct
      241 ttcttgtgat gcatcagatt cagtttgccg tctgtggtct gggtgaagaa cgacatcagg
      301 aacgggttac cagtcccggt catcgccacg acgtcaggta acgctacatc ggtatacaga
      361 taaattccca gaccgaactg gttgttggtc agtgcgcctg acagtcgaaa cttacagctc
      421 agtctgccac cccgtgtcag cagggagact gcgtcatcca ccggatgcat cagggaccag
      481 gttttattgc tctgcttggt aaccttaaac acaccatctt ccagcgcaac actgccgccg
      541 gtgatggtcc agccctgcgc agcagcctct ccggctgtcg gcagcaggga gactgtacga
      601 acggatgtgt caccatcaga cggccccgat ggcgtgtcgc cgccgggcga gggtttaatc
      661 tccggtgcgg taccgctgat gaaggcgctg gttcgaccaa ctgcgttcag aatagcggtt
      721 gccaggcgat ccgaaataat gcccctgcgc gcccatgaac tgaaatgtgt cggacgattt
      781 gacgataccc aattaccatt actacgggat tgcgcgccgt aataacctgc atcagcaata
      841 tccgggtctt ctgccggtaa gttggtgggc gtgttgttgc cgttaccgtc ggtcatgaac
      901 ggcacaaaga aaacgttgtc gctctccctg tttttgtacg cgccgtagac ggagtcatac
      961 tgtgtgccgt atgtgttttt ccagtaatac gtcgtgtcgc cacaaatcca cggtacaact
     1021 gcagcactgc cgccatggca ctgcgcgtta aatccggaaa ggtcagtacg gaactgcttc
     1081 agcatggccg tgaacaggtc cggttgctgt gcgtaggtgg cagcgctcat gtcaaattcg
     1141 ccctgcatcc agcacaccgc cagcaacaca tttttcgggt tcttctgtaa tgccgctttg
     1201 gtgcgtgcga tcaggtcctg atataacggt ttacccacac cccagcgtgc cgaatcctgg
     1261 ctggcccccg tgtccgcact gaatgtcccc tccgcgccct gggtgaatgc cgaaccacca
     1321 cgacagcatg gtaccagcag gatccccgcg ttattcggga tatacggaag cagttttttg
     1381 gcaatatgta agccctggcc gacacagccg tactgccctt tgctcaggtc tgccttcgga
     1441 tgattcagtg cgctcatatc ctgaacatca tgcaggcagt ggtctgccgg aatgatgtcg
     1501 ttaaatgcgc atgctttacc accgggagtc actgtgttgc gacgggccag ttgcttaata
     1561 cgtgggtggg gcgcatcgta agaatccgga agcggaagcc cttcaccgta agccatggca
     1621 ttggattgcc cggccagtac gatgacgtag taccactccg gctcagttgc accactgacg
     1681 accacatcac cttctgctgc aatcgcctgc atcagggtat aaggggttat ggccaccgga
     1741 ctaccaaacg gctgccagcc ctctttcagt ttatgtgtca gcttttccgc aagatctgac
     1801 ggcgacgccg ccctgacaac atcatagtgt ttaaatgcca tggttctttc caccatctga
     1861 aaaatgattc tttaaaatac ctgacatgta atacagaaaa aaacaaaacc ataccttaaa
     1921 taaaaacctc atcatcaagc agatatgcat ggataaacta caagacgaga tataaaccac
     1981 catgcattta aataaacaat aaacaacatc agaaaaataa ttctgctcta tggtttacaa
     2041 tcaaaaatat catttatact tttcagaaca tcaccagcaa gacataaaca aggaaactaa
     2101 atgaagtgga ttgtgattga tacagttatc cagccatcat gcggaatatc tttttcagtc
     2161 atatggagta aaataaaatt aataatctgg tatcaatcgg atgctttctt acctcctgaa
     2221 agtatattta cactgactca cacaggcatc atgctcaata acaaagtgct acctgtaacc
     2281 atttacaacg tagtgccatt caataaaaca ttctggaatt taatcaaaaa cagccaggaa
     2341 tgccctacaa atacagataa cgtattgaat gaatgcttta ataaccgttg cactctgcaa
     2401 atatgtcctt atggactaaa acaacaaagt ccataaggag tttactcaca tctgacaaaa
     2461 tcaatataaa cagcccctcc ggagaggggc tggagagtgg cgctatgtgc cattgcatgg
     2521 tgccgggtgc ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac
     2581 ctactcgctg attgcccctc cgcacagggg gattcaccat gcgaaatttt ttaacaaact
     2641 tcccgctggc cagacaataa tcgccagcct gaattatgag caacgtagca ttttacggga
     2701 aaactgtttt ctgcagtaaa aaggcccgcc ggagcgagcc tggaaggata gcggtcatgt
     2761 gatgccggtt tcccggtaac tcagcatcgg tatctgagtc aacgttttct ctactgggtc
     2821 atttccgata cgttccgcct tccggcagac tttcatcacg tcagaaaata tagcaccctg
     2881 agtaacagga cagtactcag aattcaggaa actgtgacac atcctgcaca gaaaagcccc
     2941 tccggagagg ggctgaagta tagcctaatt tctgtctgtc gcatggtgcc aggtgcctcc
     3001 cggtaaattc agcctgtcta ctgaatttgc atgttctctg gatcatacac tttgccagat
     3061 gccccgccgc tgagggggat tcaccatgcg atgtaatttt taacaaattc tccggcagcc
     3121 agacaatcat caagctgtgg aattgtgagg tatttaaaaa tttcaacggg taactgatac
     3181 cctgctaatc gcctgatgct ttctttttca gcaacgggaa agcaacaacc accacacccg
     3241 ccaccaaaac atcatcagac agcaccgaca ttatccggct gctgaagtcc accatcacca
     3301 ccagaaacaa caggagtgca accacagctg cttagaaggc aattgctctg tccggctgag
     3361 ctaacaacgc agaataccga taaatggacc gccatcgaga actcgaaccc cgcgcaacca
     3421 gcttcgaagg ctggcgctct atcccgatga gctaatggcg gtatgtgatg gtggcccttg
     3481 ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac tgagctaaag
     3541 ggccgggagc cgcataataa cgacgcgtaa ttaattctgc aatatcatcc gttctggctg
     3601 actaaatcct gtacttcccg aaccgtctgc tcaaaacgtc cggtctccag ctcaacgcca
     3661 atcgcacgac gcccgagcgc cagtgccgct tttaccgttg aacctgagtc cataaaaaaa
     3721 tctgcaacca ggtcacccgg acgactgctt gcgctgatta tctgctgcag catttctgcc
     3781 ggtttttcgc acggatgttt cccgggatag aactgcaccg gtttatgtgt ccacacatcc
     3841 gtgtacggca cctgcgccgt cacaccaaaa taccgccgca gatgcttata ttcactctgc
     3901 agctccacat actgccggtt cagtgacgta tacgtatcca ccagctggtg gtggggcttt
     3961 tccagttcac cgcgctgatg tttctcttct gccacccggg caaacagcga ctgtaatttc
     4021 agataatcgc tttcgttcgg tagctgccac tgactggcac tgaaccagtg cgacaccatg
     4081 tttttctttc ctgtggcatc tgcaatctgt tttgccgtta tccccagggc cgcgcgcgca
     4141 tcacgaaagt aagaaatcag cggggccatc acatgctgtt tcagtgcact gcccttcgcc
     4201 gcatacccgg catctttcgg acgatacggc ccctgataat gttccgcgaa cagaatgcgc
     4261 tctgtggcgg ggaaatacgc ccgcaggctt tccttgttgc atccgttcca gcgtccggac
     4321 ggcttcgccc agataatatg gttcagcaca ctgaagcgtt cacgcatcat gatttcgata
     4381 tcagatgcca ggcgatgacc acagaacagg taaagacttc cggcaggttt cagcacccgc
     4441 cagaactgcg ccagacactg gtccagccac ttcaggtaat catcgtcgcc cttccactgg
     4501 ttatcccagc cctcaggctt cactttaaag tacggcgggt ccgtgactat caggtcaaca
     4561 gaattttcgg gtaacgacca gataaattcc aggcagtcgg cgttg
//
LOCUS       DAJTNA010000254         4577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTNA010000254 DAJTNA010000000
VERSION     DAJTNA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.259"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..323)
                     /locus_tag="OO161_003883"
                     /pseudo
     CDS             complement(1..323)
                     /locus_tag="OO161_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(316..3576)
                     /locus_tag="OO161_003884"
     CDS             complement(316..3576)
                     /locus_tag="OO161_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5464760.1"
                     /translation="MTEDPCEDDFPVTTLKISANSQSFQSEIQRASRMGSEYYRTLQN
                     GGRHAAAAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWGSVKA
                     RLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVT
                     EAISTGLKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGV
                     ARKDLKAMADDGKLTADKVVPVLISQLGILRDEYAAMPETVSGSITKVENAFMAWVGE
                     VNEACGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTA
                     ARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNR
                     NIAARSAAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAALYTLYQNQEQAWESA
                     RQYALTIDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQ
                     YVLANPGWMTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLALMQEKAQSIQDVLA
                     GLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRL
                     PQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYIS
                     NALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTA
                     QGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQ
                     GERLRERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQE
                     AFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNK
                     VEWRSWASSVLQEISKVLMNAAIVNGIKTAANSMSGAGGFIGSIGSWLGGAVANAKGG
                     VYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMN
                     GSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            3621..3950
                     /locus_tag="OO161_003885"
     CDS             3621..3950
                     /locus_tag="OO161_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5464761.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            3937..4329
                     /gene="tnpB"
                     /locus_tag="OO161_003886"
     CDS             3937..4329
                     /gene="tnpB"
                     /locus_tag="OO161_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5464762.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKARSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTQNTLLTQ"
     gene            4336..>4577
                     /locus_tag="OO161_003887"
     CDS             4336..>4577
                     /locus_tag="OO161_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5464763.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAG"
BASE COUNT          966 a         1466 c         1075 g         1070 t
ORIGIN      
        1 aatcgtacaa ctgaaatcac tgtacctggc gttatctgtg acgctccact cccggcatac
       61 caccctcacc gtccggttat gtttcggcgg tcgccacaaa aaggcacggt aaccaccatg
      121 ccacgataaa aactcttcca gccatcgccg ggtcgcctca tccgtcaccc ggaacaccgc
      181 ctgaaacgtc ttcagtcggg cattaagtcc cgtcgggcgg cgctgttcat aaccgtcacc
      241 aaaccgtacc ctcgccaccg acggtttctc accacctgca tcccttcacg cgggaccaga
      301 tgcagcgttt ttatctcagc cactcagcat tcctccgtca cgtcgcatgg acagcatcac
      361 cgcctgcacc cgctggtcaa tcagttgcac aaggctgccc gcagcttccg gccctatctg
      421 gccattagtc ccgtcattct gaatggcgat atggtagacc ggggaataca ccagacctgc
      481 actgccgttc atactgccca ccgctcgcac acccagcgag ccatccgccg cccgggtcag
      541 gggcataatg gcttcaggac cggcctcccc catcagtccc gccccttttg caaaggcaaa
      601 gtacgtgggc gtatccacaa tactgttgct gtacgcactc aggtttgccg aggtatacac
      661 accgcctttt gcattggcca ccgcaccgcc cagccagcta ccaatactgc cgataaatcc
      721 tcccgctccg gacatactgt ttgccgccgt cttgatcccg ttgacaatcg cggcattcat
      781 aagaactttt gatatttcct gcagcactga tgaggcccag ctgcgccatt ccactttatt
      841 tccgttcagc atctccgtga tgttattcac catccctgag ataccctccg tcgcaagctg
      901 tgctgcctgt gaggcgtaat cggacgcatt atccacccag ttactgaatc cctcctgcag
      961 ccctttctgc cagtccgcac gctgcacatc cgattcggca taaaaggctt cctgctcttt
     1021 cagacgttca ctcagatact gcgcgttctg tgccagagcc tgtctgtaaa aatcctcact
     1081 gatatccccg gtctgatact gagactgaag gtccgcatcc ttctggcgga aactgtcgcg
     1141 gatctgctgc aactcccgca tgcgttcacg gagccgctct ccctgcccgt accccagcag
     1201 ttccgcttca tttgatgcac gcgcagccac attatcattc ttcagggtct cttcccggga
     1261 ccgcaactgc tcccggattt tttgctggtc aatcagggcc gcattgcgca gcagttcctg
     1321 cttctgcatc tccgtcaggg ttttcagttc gccctgcgca gtctggtatt tcagcttcgc
     1381 cagctctgta ttctgccccg ccagtgccag ttgctctttc tgctgcttca gcagccggga
     1441 aaaactgtct tccgcttttt ccgtctctga ttttccaccc cgggatttgg gtttattcgc
     1501 ctcgttattg cgccaggctt ccagggcatt actgatataa cgttgtctcg cctcctgata
     1561 cggatcaccc acaaaaccga ggtcatccgc cgcatacccc agtcggacac gctctttttc
     1621 ttcccctttc agtctggaca gggccagctc acgctctgtt tttgtcaggg cactctgctg
     1681 tttatcatcc agagtggcct gtggcagccg taacggcaca ttcaccagtc cctgccgctg
     1741 ctgaagcagt tcattaccca gccccagcag acggttgaat tccgtatgct gaccgttcat
     1801 aaccagcatg gactggtaca ccttattctg ctctgccgcc tgctgacgaa ttaacgccac
     1861 acgacggtct tccagcccgg caagcacatc ctgaatggac tgcgcttttt cctgcatcag
     1921 tgccagacgg gactgctcaa cggcaagctg ctctgttgcc tgagcaagcc cttccgttac
     1981 ggtcttcacc gaggtcagat ggtttatcat gaatccgtca ccggtcatcc agcccgggtt
     2041 cgccagaaca tactgatatc ctgcgatttt ttcctgcagg gatttcaccc gactagcctg
     2101 ttcatcaatc agccggttct gctctgtcag cgccgcccgt gttcgtcctt cattatctga
     2161 ggcttcaggc aaagacattg acggcgtttt atgcgcgatt tcatctatcg tcagtgcata
     2221 ctggcgcgca gactcccagg cctgctcctg attctggtac agcgtgtaca atgctgcagc
     2281 ccccagcatc accagtccgg gtacgccacc aaccagcccc agcgcaccgc tcatcagacg
     2341 tgagcccacc gccgttgtac tgttcagcgc attctgggcg gcgcttctgg cagcaatatt
     2401 tctgttcagg cgttcctgtg tggccgccag acgggcctct gcagcaatct gcatctccgt
     2461 cccgcgggct gccgccacgg cctgctgagc acggtacacg gctgcccttg cccgcgccgt
     2521 ggcaatctgc gttcccctga actgtgcttc cgccagtgca acttcattac gtgcagccgt
     2581 cacaagtcct gccgtggcag acatcgctcc ggaggccata ttgccaaagt accgggcaac
     2641 cccgacggca accagcgccc ccacagctgt tgccacatta tcaatctgtc cggcaacacc
     2701 gttcagcacg ccggagagcg tttttgtcac cccgcaggcc tcattcactt cgcccaccca
     2761 ggccataaag gcgttttcca ccttcgtgat actaccggaa accgtttccg gcatggccgc
     2821 atattcatca cgtaatatcc ccagctggct gattaacaca gggaccactt tatccgctgt
     2881 cagttttccg tcatccgcca ttgccttcag atctttacgg gccacaccca tccccgcagc
     2941 aagcgcgcgg atcacccggt cgccattttc gttaacagca ttaaattctt cgccacgcag
     3001 tacaccctgt gccagtgcct ggctgaactg ggtgatcacc gaaccggatt ccgcaactcc
     3061 agcccctgac agtttcagtc ctgtcgaaat ggcctccgtc accttcagca catcatcagc
     3121 actgtaacca tattcacgca tcgaggcagc cgaacgggca aacagggccg cattatccga
     3181 aaacgcggta cctgttcgct gactgatatc catcagcact ttctgtgatg acgagaattc
     3241 atccgatgac tgagatgcct gtttcagacg ggccttcacg gaaccccact catcggccag
     3301 cgaaatcagg tgtccggtgg caaaggcacc ggcaaatgcg ccagccattc cgacagcaga
     3361 accgcgaatt tccgtcaact gactgttcag ttctgcaagg gcgcggcgct gttcccgtgc
     3421 ggcagcagcg gcatgacgtc cgccattctg cagggtccgg taatattcac tgcccatacg
     3481 ggacgcccgc tggatctccg actggaatga ctgtgaattt gccgaaattt tgagtgtcgt
     3541 tacggggaag tcatcttcac agggatcttc ggtcagttag tattaccgac atccattcac
     3601 caacttaccg gagataatcg atgtcaaaac cccgatggac cattgaacag aaaaggcagc
     3661 acgttgctgc ctggcgcgcc agtggtctta cacgtcagca atactgtgaa ctcaacgata
     3721 ttcccttcac atcacttcgc gaatggccgc aggatgtcgc aaaagctgaa cgccgggcaa
     3781 atgaaccgac cgttcttccc gttcatattg cgccaccact gcatgctgat gtaccacagc
     3841 ccgtcacgaa tgagccagtc acgctcttcc ttcccggtgg agtacggatg tgctgtcagc
     3901 cgtcacaact taccgatgtc ttcagggcac tcaaatatgc tcagtcctga taatgtcttc
     3961 atcgcaatta aacctgttga catgcgccgg ggcatcgact cactgacgca gtatatacag
     4021 gatgaactcc ggtcgacatg gcatgaggga gccgccttcg tctttgttaa caaagcccgt
     4081 tcgcgtatca aagttctccg gtgggataaa cacggggtgt ggttgtgtac ccgccgtctg
     4141 cacaaaggca gcttccgctg gccacgtgca aatgacgctg cctggcacct cactcccgac
     4201 gagtttaact ggctgattgc cggtgttgac tggcagcagg ttaagggaca tgacctgacg
     4261 aaatgggtct ggcagaatga acctgaactg cgccctgaaa acacgcaaaa tacactgcta
     4321 actcagtgaa aaataatgaa tatccgtatc tggagtggta tactcccctg tatggatatc
     4381 tccgctctca acaccacgaa tgacatcgaa aaactgcgtg ctatggcact tgccatggta
     4441 caagaagtca tgtcggagaa tgccgaaaaa gagcgggaat tactggagaa aagccggcgc
     4501 atccagcttc tggaagaaat gctgaaactg gttcgtcaac agcgcttcgg aaaaaaatgt
     4561 gaaacgctgg ctggtat
//
LOCUS       DAJTNA010000255         4561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTNA010000255 DAJTNA010000000
VERSION     DAJTNA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.260"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            229..450
                     /locus_tag="OO161_003888"
                     /pseudo
     CDS             229..450
                     /locus_tag="OO161_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(471..533)
                     /locus_tag="OO161_003889"
                     /pseudo
     CDS             complement(471..533)
                     /locus_tag="OO161_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            984..2396
                     /gene="ospC1"
                     /locus_tag="OO161_003890"
     CDS             984..2396
                     /gene="ospC1"
                     /locus_tag="OO161_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5464764.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLTPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIAIQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHDLYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2500..2648)
                     /locus_tag="OO161_003891"
                     /pseudo
     CDS             complement(2500..2648)
                     /locus_tag="OO161_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2724..4421
                     /gene="ospD3"
                     /locus_tag="OO161_003892"
     CDS             2724..4421
                     /gene="ospD3"
                     /locus_tag="OO161_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5464765.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1604 a          812 c          834 g         1311 t
ORIGIN      
        1 aattatctaa ccttgtcttg ttacatccaa attgccatcg acagttacac agttgtgaag
       61 tcggctctct caaaaagggc ttataaaggc ttgagcggtg taccgggaaa ccggtatgca
      121 ccgttcttag gggagggcag tccagcaatg aactgtcctt acccgacaaa catccaaaac
      181 gaacggaacg tgctggaatc cgcatataac ccgttgtaaa gtgaggaaat ggacacctca
      241 cttgctcatg agaacgcccg cctgcgggca ctgttgcaga cgcaacagga cactatccac
      301 cagatggctg aatacaaccg cctgctctca cagcggatgg cggcttatgc ttccgaaatc
      361 aaccgactga aggcgcaggt tgcgaaactg cagcgtatgc agttcggtaa aagctcagaa
      421 aaacttcgcg caaaaaccga acggcagatt ccattttcac gggcgatcta cgccacacag
      481 gctctgggtt gcagtgactg ctccattaag gcgattttga actcataagg gaagtaacaa
      541 aaaaactgca aaagtacaat aattatatat ggccaaacta acctctatta aaaaaacatc
      601 agtttttcac acttagttga gcttcatgaa aaaatcatcg aggatttcat tgcagaatac
      661 acattccact aactggaagc actccataag aggaatcaga gaaagtatcg tttttttata
      721 gtaaaattca ttgctgttca ataaaattga tatagttatt aaattatgaa aataatttca
      781 caaaaaagat taacttgagt tcttaagcat cggattttag atattttgaa atatctggca
      841 tcacataaca tcaaaagtat ataataccct tatctaatta catgcagtat attgtatata
      901 tactcaaaaa ttgtaattca taccctacta tatcttgtgg ggaaagatat taattaaaac
      961 tgttttcata taaggttcat tttatgaata tatcagaaac actgaactca gcaaataccc
     1021 aatgcaatat agattctatg gataacagat tacatacatt gtttccaaaa gtgacatcag
     1081 tgcgaaacgc tgcacaacaa actatgccag atgaaaaaaa tttaaaagat agtgcaaata
     1141 ttattaaaga tttctttagg aaaactatag cagcacagag ttatagtaga atgttctctc
     1201 aaggctctaa ctttaaatct ttaaatatag caattgatgc accatcagac gctaaagcct
     1261 catttaaggc tattgagcac cttgacagat tatcgaagca ttatatatct gaaataaggg
     1321 aaaaacttca tcctctttct gcagaggaac tcaatttgct ttcgctaatt attaattctg
     1381 atttaatctt cagacatcaa agtaattctg atttgtctga taaaatttta aacattaagt
     1441 cattcaataa aattcagtct gaaggaatat gcacaaaacg aaacacatac gctgatgata
     1501 taaaaaaaat agctaatcat gactttgtgt tttttggcgt tgaaatctct agccatcaga
     1561 aaaaacaccc cctgaataca aaacatcaca ctgttgattt tggtgcaaat gcgtatatca
     1621 ttgatcatga ctctccatat ggatatatga cattaaccga tcactttgat aatgctattc
     1681 cacctgtttt ttaccatgag caccaatcat ttttagataa attttcagag gttaataaag
     1741 aagttagtcg atacgtacat ggaagtaaag gaattataga tgtaccaata ttcaatacta
     1801 aagatatgaa gttagggctc ggattatacc tgattgactt tattagaaaa agtgaagacc
     1861 aaagcttcaa ggagttttgc tatggaaaaa atcttacccc tgtggatctg gatagaatca
     1921 taaactttgt ttttcagcca gagtaccata tacctaggat ggtaagtaca gaaaacttca
     1981 aaaaagttaa gattagagaa atatccttag aggaggctgt tacagcatct aattacgaag
     2041 aaattaacaa gcaggtcact aacaaaaaaa ttgctatcca gactcttttt ctttcgatta
     2101 ctaatcaaaa agaggatgtc gccttatata tattatctaa ttttgagata actagacaag
     2161 atgttatttc cataaagcat gacttgtatg acattgagta tctacttagc gctcataatt
     2221 caagctgtaa agtacttgag tattttatca ataagggatt ggttgatgta aacacaaagt
     2281 tcaaaaaaac taatagtggg gattgtatgt tggataacgc aataaaatat gagaatgcag
     2341 aaatgataaa actattattg aaatatggtg caacatctga caataaatat atttaatcaa
     2401 aattgaatat cgtttagcaa aaggcagtat ttgcacaaaa tatcgatata ttgtttattg
     2461 tcagtatggc tcaatgtgat aatagttgga aagtttgatg ggtttcgccc cgttgtagcg
     2521 gtagtcgagc tggaaggtct tcaggcactg cttacagcga tagagcagcc cccctgaact
     2581 ggaatggccg ttccggatac cccctgagtt tcagagtaac ggggacaaac cacatcaatc
     2641 tttgccatca atcatccaaa gggcaaagag tacaacaaca ctaagtctgc gtcacaaccc
     2701 atcaatgaaa ggaatatata catatgccat cagtaaattt aatcccatca aggaaaatat
     2761 gtttgcaaaa tatgataaat aaagacaacg tctctgttga gacaatccag tctctattgc
     2821 actcaaaaca attgccatat ttttctgaca agaggagttt tttattaaat ctaaattgcc
     2881 aagttaccga tcactctgga agacttattg tctgtcgaca tttagcttcc tactggatag
     2941 cacagtttaa caaaagtagt ggtcacgtgg attatcatca ctttgctttt ccggatgaaa
     3001 ttaaaaatta tgtttcagtg agtgaagaag aaaaggctat taatgtgcct gctattattt
     3061 attttgttga aaacggttca tggggagata ttatttttta tattttcaat gaaatgattt
     3121 ttcattccga aaaaagcaga gcactagaaa taagtacatc aaatcacaat atggcattag
     3181 gcttgaagat taaagaaact aaaaatgggg gagattttgt cattcagctt tatgatccca
     3241 accatacagc aactcattta cgagcagagt ttaacaaatt taacttggct aaaataaaaa
     3301 aactgactgt agataatttt cttgatgaaa aacatcagaa atgttatggt cttatatccg
     3361 acggtatgtc tatatttgtg gacagacata ctccaacaag catgtcctcc ataatcagat
     3421 ggcctaataa tttacttcac cccaaagtta tttatcacgc gatgcgtatg ggattgactg
     3481 agctaatcca aaaagtaaca agagtcgtac aactatctga cctttcagac aatacgttag
     3541 aattactttt ggcagccaaa aatgacgatg gtttgtcagg attgctttta gctttacaaa
     3601 atgggcattc agatacaatc ttagcatacg gagaactcct ggaaacttct ggacttaacc
     3661 ttgataaaac ggtagaacta ctaactgcgg aaggaatggg aggacgaata tcgggtttat
     3721 cccaagcact tcaaaatggg catgcagaaa ctatcaaaac atacggaagg cttctcaaga
     3781 agagagcaat aaatatcgaa tacaataagc tgaaaaattt gctgaccgct tattattatg
     3841 atgaagtaca cagacagata cccggactaa tgtttgctct tcaaaatgga catgcagatg
     3901 ctatacgcgc atacggtgag ctcattctta gcccccctct cctcaactca gaggatattg
     3961 taaatttgct ggcctcaagg agatatgaca atgttcccgg acttctgtta gcattgaata
     4021 atggacaggc tgatgcaatc ttagcttatg gtgatatatt gaatgaggca aaacttaact
     4081 tggataaaaa agcagagctg ttagaagcga aagattctaa tggtttatct ggattgtttg
     4141 tagccttgca taatggatgt gtagaaacaa ttattgctta tgggaaaata cttcacactg
     4201 cagaccttac tccacatcag gcatcaaaat tactggcggc agaaggccca aatggggtat
     4261 ctggattaat tatagctttt caaaatagga attttgaggc aataaaaact tatatgggaa
     4321 taataaaaaa tgaaaatatt acacctgaag aaatagcaga acacttggac aaaaaaaatg
     4381 gaagtgattt tctagaaatt atgaagaata taaaaagctg aatattatct cgtggtgata
     4441 cactgaattt gaggtaaccg gctcatttaa accgtctggt ctgttacctc cggttccaca
     4501 aaaataatgt ccatcatttt gaagtggaca ctatcgtatg gaataccgga cctggatcac
     4561 t
//
LOCUS       DAJTNA010000256         4530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTNA010000256 DAJTNA010000000
VERSION     DAJTNA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.261"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            291..663
                     /locus_tag="OO161_003893"
                     /pseudo
     CDS             291..663
                     /locus_tag="OO161_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1225..2172)
                     /gene="icsP"
                     /locus_tag="OO161_003894"
     CDS             complement(1225..2172)
                     /gene="icsP"
                     /locus_tag="OO161_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5464766.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSAWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3553..3705)
                     /locus_tag="OO161_003895"
                     /pseudo
     CDS             complement(3553..3705)
                     /locus_tag="OO161_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3761..4453
                     /gene="ospZ"
                     /locus_tag="OO161_003896"
     CDS             3761..4453
                     /gene="ospZ"
                     /locus_tag="OO161_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR5464767.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCSEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENRDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RLSQIKAQISVT"
BASE COUNT         1396 a          884 c          883 g         1367 t
ORIGIN      
        1 gcagcgggct tgtaatgggt taagtgataa cagatgtctg gaaatatagg ggcaaatcca
       61 gctgcgatct ggttcaacaa tatgcagatg gtacacaacc tctttaacca gcccatccgg
      121 attattcaga gccgcttccg caatccggaa taaaagtgct gttttaccgt gaaccctctg
      181 aaaatcagcg agcagtgttt taaccagccg acgttcagag cgtactgaca gacggtggac
      241 aatttgaata aacagttcaa tcagaccgta gatgctctcc cgttggcgac atgctgggag
      301 cggtggaaca ccgcttcggc aacgagcttc cggcgtctcc agtgaagtga ctgacggata
      361 atggttcatg ctaccgggct aatgaaacac gccagttcac ccggatgttg ggactttgtt
      421 gaaccgaaga acacggcggt gcggagtccg gagagtaaca gaatagcaaa gagcttcgtg
      481 aaaacaataa agtgtgacta catcagtatc atgcccaaac ctgacgggtt aacggcagca
      541 aagaaccttg cagaggcatt cgagcattat aacgaatggc atccgcatag tgcgctggat
      601 tatcgctcgc cacgggaata tctgcggcag cgggccaatg ataacaggta tctggaaata
      661 taggggcaaa gccacccacg aacgatccag catataatca agaaattaga tttcatttaa
      721 cataagatag attatgtgca caaagaggca tttccttaat ttctgaattt acaggctctt
      781 ttgctctctt tgctatgctg tggatttgga aagatagagt gctggctgat tatttaggta
      841 ttttcatcag catgtccctg atagcactgt tccatcagcg ctttcaggcg ataatagtag
      901 accttgggta gcctctgccc atgtgtaact tgataccccg agaacagcca cgatgatcta
      961 agcggttctt atttctccgg tctcagggtc ggtaatgcca acagtaagtc cacagtaatc
     1021 aacgaagagt tttttgaacc gccccggaaa tcctggagac taaacgccct gagaaagagg
     1081 taaacagaat gactaaaaat acacgttttt cccccgaagt ccgtcaacgg gcagttcgta
     1141 tggttctgga aagtcagggc gaatatgact cacaatgggc ggcaattttt agccatgaaa
     1201 acgtaatcaa ctcggtgaac caattcaaaa aatatacttt atacctgcgg aagtgagaaa
     1261 accaatatat tcaatacttg cactattttt aatggtgcca gatatattgg tactttgctc
     1321 ataaagagat gtatcacctt ttttattaga aatgtaattt ctggagcctt ctatataaaa
     1381 ttttgcactg ggggtgacat agtatccaat attcgcagca acagagcagt aattttggtt
     1441 ttttatttca tctttaaaaa ttgtctcagt ctgatagtgt ttatctgtat cagatgaacg
     1501 aacccaagca ctatatttca gttctgctcc aaactcaaaa ttctcatggc ggtaattagc
     1561 agttaatcca atataaggta ttttgaagag ctgtttgtaa cctattgttc tttcaccact
     1621 aggatgtgcc ccttttttat tcctgttccc cccgttttca ctataaatat aactccctcc
     1681 cattgcatta aaactgtaac gactctcttg gtaacctgct attagtccaa gtcgataatc
     1741 caaattattt aataaccaac ctttaagatt cagctcgaat tcgttagcat cacggagatg
     1801 cgtattggga tgccagctct ggtcggtcca tacctgaggg gtattggaat tgttccagtc
     1861 tttatcaacc atgcttgctt tctggttacc taaagtagtc caaccggaaa ctccgaaaga
     1921 cacttttgga attagcttcc aatcgatgcc acctctaaca atagtcgcat tactgtattt
     1981 ccagtccagt tgactaattt ttctcccccc ttccttggga tgataaacgc gttcttttgt
     2041 tttgccactc agagatccta gactaatatc cgtactgatg ttatccggta tgaaaagtgg
     2101 atagttagtg gtagcatgtg tagtaaagat cgcaggtaca caaagtgcaa gtacaaggaa
     2161 ttttaatttc atcgagaact ctactttttt ggttgaaatg tccatgatct tcttacttta
     2221 taagataaac gtcaatgcca agcattattt taattaaata tttgtttatg ttaccaagaa
     2281 catatctttt attataatat ttattgaact tttgtaaaaa taaaaaaata cctcattgtg
     2341 cgaataaagt aacgggggcc atagatttct ctacctacct tttggtgttc atacctgagg
     2401 ctgatagttt caggattccg cgacaattct actcaccttc ttgttctctc gccaatatga
     2461 aatcaatcag aaaagcgttc cataagaaca acgataagac aataaattgt catcacggct
     2521 gcaatagcca acgtgccttt cagtaccaac atgacaacct cctgtatttg acgtaacgtg
     2581 tgctgataaa tatgtatttg tatctcaacc gactcaatac aacacttaag tatagttttt
     2641 cagattttgt ttttcccgtg aaatattatg gaaatggcag aagcaataca ttgaattccg
     2701 attcgagtga atacggctag cgggcacctc actttagcac tgaagccatc aacttttatt
     2761 tgacttattt caatggaaag ctttctcact atcagtacag gtaattccca tccttttaaa
     2821 gtcgagagtc agactgcgac taatacagtt aaagtgacca aactcagtta tcctctacac
     2881 attttacctc atgtttactt ggacaattca ttggtgcttt tataaaagaa taaatattct
     2941 actaatttaa tgcatcacct aaaatagtat tttttataat agaactgtat ctatagtata
     3001 ttattaaagc ttaattattt attatgcttt aatgtggttg tccgattaag gactaatcct
     3061 tgaaaacgcc gtatagggcg ggagttcact ctattgcgat aacgtaaccg tacaatattg
     3121 tgcgtttccc aaattgacca ctactgaact attctgggat ttaaatgcaa taaaatggtt
     3181 ggttgaaggt cgtggtgatg atgttagtcc ttttgagaat attccagtgc cagatattac
     3241 ttataataaa gaggcttggc agtttggata agctattgtc cttattccac taatacaaac
     3301 tttttgtaaa atatattaaa tatacttcat ttcatactga aatccccgga agtattcaat
     3361 caaacaatta caccataaaa ggaagaggga gccccaatta agttatcaag catgtttatg
     3421 atacctgcag ggtataaatt ttctggctgc aagcagacac atataaaacg tctttattga
     3481 tcctacccac ataatatgga cgcagtcgta agtgaggccc tgtgagccta cccacgtaat
     3541 atggacacag gcctaagcga ggttctggtt ttcaaattgt tccggactga ggccgccaca
     3601 ccaacggtgc cgtcgccagc gattgtaatc gcactcgata taattaaaca ctgttgcccg
     3661 cactatttcc cgatagataa agtgttctcc gtggagacat tccacttagg aacaattata
     3721 ttgttcatgg taatgtttat atttgagtat agagattaaa atgattagtc ccatcaagaa
     3781 tattaagaac gtatttccaa taaatactgc aaatactgaa tatatagtga gaaatattta
     3841 tcctagagtt gaacatggtt actttaatga atcaccaaac atatatgaca agaagtatat
     3901 ctctggtata accagaagta tggcacaatt aaaaatagag gagtttatta atgagaaatc
     3961 cagacgactt aattatatga agacaatgta ttctccatgt tcggaagatt ttcagcctat
     4021 ttccagagac gaagccagta taccggaagg aagctggttg acagttatat ctggaaaacg
     4081 tcctatgggg caattttctg tggatagttt gtaccatcct gacttacatg cattatgtga
     4141 gctcccagag attagttgta agatcttccc taaagaaaat agggattttt tgtatataat
     4201 tgttgtattc agaaatgatt ctccccaagg agaactacgg gcaaatcggt ttatagaact
     4261 atatgatata aaaagagaga ttatgcaagt gttaagagat gagtcgccag agttaaagtc
     4321 tattaaatct gagattatta ttgctcgtga aatgggagaa ctttttagtt atgcatcaga
     4381 ggaaatagat agctacataa aacaaatgaa tgacaggctt tctcaaatca aggctcaaat
     4441 atctgttact taatgtatat taattgtatg aagtggtcaa caaaaactgg ccaccgagtt
     4501 agagttttta cagtatcgat tttccgattc
//
LOCUS       DAJTNA010000257         4433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTNA010000257 DAJTNA010000000
VERSION     DAJTNA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.262"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..1086
                     /gene="nadB"
                     /locus_tag="OO161_003897"
     CDS             <1..1086
                     /gene="nadB"
                     /locus_tag="OO161_003897"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR5464768.1"
                     /translation="VGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSGD
                     GIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPDF
                     DERGELAPRDIVARAIDHEMKRLGANCMFLDISHKPADFIRQHFPMIYEKLLGLGIDL
                     TQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECLV
                     YGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIVR
                     TTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRGL
                     HFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1071..1808)
                     /gene="trmN"
                     /locus_tag="OO161_003898"
     CDS             complement(1071..1808)
                     /gene="trmN"
                     /locus_tag="OO161_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5464769.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1940..3274
                     /gene="srmB"
                     /locus_tag="OO161_003899"
     CDS             1940..3274
                     /gene="srmB"
                     /locus_tag="OO161_003899"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5464770.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHTEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3483..4364)
                     /locus_tag="OO161_003900"
     CDS             complement(3483..4364)
                     /locus_tag="OO161_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5464771.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCIEECFLPG"
BASE COUNT         1127 a         1207 c         1124 g          975 t
ORIGIN      
        1 gtaggcgcgt gggtatggaa ccgaaataaa gaaacggtgg aaacctgcca cgcaaaagca
       61 gtggtactgg caaccggcgg tgcgtcgaag gtgtatcagt acaccaccaa tccggatatt
      121 tcttctggcg atggcattgc tatggcgtgg cgcgcgggct gtcgggttgc caatctcgaa
      181 tttaatcagt tccaccctac cgcactgtat cacccacagg cacgcaattt cctgttaacg
      241 gaagcactgc gcggcgaagg cgcttatctc aagcgcccgg atggcacgcg ttttatgccc
      301 gattttgatg agcgcggcga actggccccg cgcgatattg tcgcccgcgc cattgaccat
      361 gaaatgaaac gcctcggcgc aaattgtatg ttcctcgaca tcagccataa gcccgccgat
      421 tttattcgcc agcatttccc gatgatttat gaaaaattgc tcgggctggg gattgatctc
      481 acacaagaac cggtaccgat tgtgcctgct gcacactata cctgcggtgg tgtaatggtt
      541 gatgatcatg ggcgtacgga cgtcgagggc ttgtatgcca ttggcgaggt gagttatacc
      601 ggcttacacg gcgctaaccg catggcctcg aattcattgc tggagtgtct ggtctatggc
      661 tggtcggcgg cagaagatat caccagacgt atgcctgatg cccacggcgt cagtacgtta
      721 ccaccgtggg atgaaagccg cgttgagaac cctgacgaac gggtagtaat tcagcataac
      781 tggcacgagc tacggctgtt tatgtgggat tacgtcggca ttgtgcgcac aacaaaacgt
      841 cttgagcgcg ccctgcggcg tatcaatatg ttgcaacagg aaatagacga atattacgcc
      901 cacttccgcg tgtcgaataa tttgctggag ctgcgtaatc tggtgcaggt tgcggaattg
      961 attgttcgct gtgccatgat gcgcaaggag agtcgtggcc tgcatttcac actggattat
     1021 ccggaactgc tcacccattc cggtccgtcg atcctttccc ccggcaatca ttacataaac
     1081 agataaaaag cctgggtcag cgccgtatac gcttcggaat agttctggtc tggcccacga
     1141 atgactaagc gatcgctaaa gcattctccc gcctgcgggg agaatgccag cagcacccga
     1201 tgcggcagtc gcgcttcgtt ttccgccaca tccgtccgca aacgtaaatg ccagcccatg
     1261 cttaatgcca gctccgtaaa accattacca atctgctctg gcagcactac gcagaaaaat
     1321 ccctcttcgg taatgcactc cgccgcacag gtcagcagcg atgggtgatc aagcgtagtg
     1381 gtatagcgag cctgttcccg ttgagggatc gcgcactcta ctccctgctg atagtaaggg
     1441 gggttactga tgattaaatc gaagcgtact gtctgctgtg tgagccactg ctgaatatcc
     1501 gccgtatgga cgttaatccg ctctgcccac ggggactggt tgatattttc ctgcgcctgc
     1561 gcggcagctt cactttccag ctcaacggca tcaattatca cgctgtcact ggttcgctgc
     1621 gccagcatca gtgccagcaa cccgctaccc gcgccgatat caaggcaacg tttcacccca
     1681 gccaccggtg cccatgcgcc cagcaaaatg ccatccgttc ccactttcat cgcacagcga
     1741 tcgtgagcaa caaaaaactg tttaaaagta aatccattac gacgaagcac ggatgtagac
     1801 tgtgacatga aaataaaacc ttgcaggaaa aacggcgata gcaccgggtg agaacaatac
     1861 ccgagaagcg atatccatac aaacagatga agattgcagc cgtaacgtct ataatcagcg
     1921 ccccacacag aggtagaaca tgactgtaac gactttttcc gaacttgaac tcgacgaaag
     1981 cctgctggaa gccctccagg ataaaggttt cactcgcccg accgccattc aggctgccgc
     2041 tattccgcct gcgctcgatg gccgtgatgt actcggttct gcgccgacag gcaccggtaa
     2101 aacggcggcg tatctgctgc cagcgttgca gcacctgctc gatttcccgc gtaagaaatc
     2161 cggtccgccg cgtattttga tcctcacccc aactcgcgag ctggcgatgc aggtggccga
     2221 tcatgcccgc gaactggcga aacatacgca tctggatatc gccaccatta ccggcggcgt
     2281 agcctatatg aaccacacgg aagtgttcag cgaaaatcag gacatcgtgg tcgccacgac
     2341 cggacgtctg ctgcaataca taaaagaaga gaacttcgat tgccgcgcgg ttgaaacgct
     2401 gatcctcgac gaagcagacc gtatgctgga tatgggcttc gctcaggata tcgaacatat
     2461 tgctggcgaa acgcgctggc gtaaacagac cctgctcttt tcggcaacgc tggaaggcga
     2521 tgcgattcag gactttgccg agcgtctgct ggaagatccg gtggaggttt ctgccaatcc
     2581 gtccacccgt gagcgcaaaa aaattcatca gtggtattac cgcgccgatg acctggaaca
     2641 taaaaccgca ttgctggtgc atctgttaaa acagccggaa gcgacccgct caattgtgtt
     2701 tgtgcgtaag cgtgaacgtg tgcatgagct ggcaaactgg ctgcgcgaag cgggcatcaa
     2761 caactgctat ctcgaaggtg agatggtaca gggcaagcgt aatgaagcga ttaaacgcct
     2821 gaccgaaggc cgcgtaaacg tactggtagc aaccgatgtt gccgcgcgcg gtatcgacat
     2881 tccagacgtc agccacgtct ttaacttcga tatgccgcgc agtggtgata cttatttgca
     2941 ccgtatcgga cgtaccgcgc gcgccggtcg taaaggcacc gcgatttcgc tggtggaagc
     3001 ccatgaccat ctgctgcttg gcaaagttgg ccgttatatt gaagagccga taaaagctcg
     3061 cgtcattgag gaactacgcc cgaaaacgcg tacgccaagc gagaagcaga ccggcaagcc
     3121 atcgaagaaa gtactggcaa aacgcgccga gaagaaaaaa gctaaagaga aagagaagcc
     3181 gcgggtgaaa aaacgccatc gcgacaccaa aaatattggt aagcgccgta aaccgagcgg
     3241 aacgggcgtg ccaccgcaaa cgacagaaga gtaatcttaa ttccgggttt aagcccggaa
     3301 ttaaaagtgc tggcaaacgc aaaactgcct gatgcgctac gcttatcagg cctacatggc
     3361 tcatgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttc acgccgcatc
     3421 cggcatgaac aaaacgcact tcgtcgacaa gttgggtgcc gggtttaagc ccggcattat
     3481 ttttatcccg gcaaaaaaca ctcttcaata cactgaataa aagtgtgcat cgccgggcta
     3541 accgcttttc cagcatggtg tgcgcacatg gccgtaatgg tctgcgactg ttcgccaaaa
     3601 ggcaattcaa ttaattctcc gctctcaagc tctttttcca ccgcaaagcg gggcaagtaa
     3661 ctgacaccga tattcgccgc gacacaacgt ttgatacttt caatgcttaa cagctcaatg
     3721 gtgttttcca ccgtgatccg ccgctgacgc agcgtgctct caaatatctg ccggaagaca
     3781 cattgcggtt cgttgataat aaagctacag gcgttatgtc ttcccggctc agtaaaatcg
     3841 acatctgcaa tttgcggtga agccaccagc accagcgatt gttcacccaa ctctcgtcgg
     3901 ttcagggcat catcattccc tacgcgataa aagacgccga catcggcctc atcattcagt
     3961 agcgcatcac ggatcacgta acagttcagc gactgcaacg ataaacgcac ttttggtgcg
     4021 cgttgccgaa aacgctgcaa cacctgcggc attcggtaag agagcaacgt ttcgcccgaa
     4081 acaacgcgta gttccccgtc cggatctgac tcctttttgg cagcctcacg aagcgtatcc
     4141 atcactcggg taagttcata aatgtgcggc agcaactttt ttccttcgcg ggtaaggcac
     4201 atccgtcggc caattttctc aaataactgg actgaaaact cctgctcaag ctgctgaata
     4261 tgaaaagtca ccgtcgattg tgtacagcac aatttttgcg aagctcgcaa aaaggaaccc
     4321 tcttccacca cggttttaag cgtaataaaa cggcgcagat ccataacccc aaacctatcg
     4381 aaaatatcga atctagaata taaaaacatt cattttttaa atgttctgtg tcg
//
LOCUS       DAJTNA010000258         4403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTNA010000258 DAJTNA010000000
VERSION     DAJTNA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.264"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..227)
                     /locus_tag="OO161_003901"
     CDS             complement(<1..227)
                     /locus_tag="OO161_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5464772.1"
                     /translation="MKSVFTISASLAISLMQCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAA"
     gene            604..2520
                     /gene="dnaK"
                     /locus_tag="OO161_003902"
     CDS             604..2520
                     /gene="dnaK"
                     /locus_tag="OO161_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5464773.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2609..3724
                     /gene="dnaJ"
                     /locus_tag="OO161_003903"
     CDS             2609..3724
                     /gene="dnaJ"
                     /locus_tag="OO161_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5464774.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGADFSDIFGDVFGDIFGGGRG
                     RQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQTCPTCH
                     GSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPAGVDTG
                     DRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAALGGEIE
                     VPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLNEKQKQ
                     LLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3828..3980)
                     /gene="mokC"
                     /locus_tag="OO161_003904"
     CDS             complement(3828..3980)
                     /gene="mokC"
                     /locus_tag="OO161_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5464775.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1172 a         1063 c         1143 g         1025 t
ORIGIN      
        1 gcggctttga aataaaccga tgcaccgctt aactgtaaat cgccatggtc ggcagagagt
       61 tgtatgcgtt tcacaatgcg acaaacagga agtttcagcg ccagatcgtt ggtttcgtta
      121 cgcggcattg caatgacgcc gaggagttta tggtcgtttg cttgcgccgt gcagcactgc
      181 atcaggctaa tcgccaggct ggcggaaatc gtaaaaacgg atttcataag gattctctta
      241 gtgggaagag gtagagggat gaatacccac tagtttactg ctgataaaga gaagattcag
      301 gtacgtaatc ttttcttttt attacaattt tttgatgaat gccttggctg cgattcattc
      361 tttatatgaa taaaattgct gtcaatttta cgtcttgtcc tgccatatcg cgaaatttct
      421 gcgcaaaagc acaaaaaatt tttgcatctc ccccttgatg acgtgggtta cgaccccatt
      481 tagtagtcaa ccgcagtgag tgagtctgca aaaaaatgaa attgggcagt tgaaaccaga
      541 catttcgccc ctattacaga ctcacaacca catgatgacc gaatatatag tggagacgtt
      601 tagatgggta aaataattgg tatcgacctg ggtactacca actcttgtgt agcgattatg
      661 gatggcacca ctcctcgtgt gctggagaac gccgaaggcg atcgcaccac gccttctatt
      721 attgcctata cccaggatgg tgaaactctg gttggtcagc cggctaaacg tcaggcagtg
      781 acgaacccgc aaaacaccct gtttgcgatt aaacgcctga ttggccgccg cttccaggac
      841 gaagaagtac agcgtgatgt ttccatcatg ccgttcaaaa ttattgctgc tgataacggc
      901 gacgcatggg tcgaagttaa aggccagaaa atggcaccgc cgcagatttc tgctgaagtg
      961 ctgaaaaaaa tgaagaaaac cgctgaagat tacttgggtg aaccggtaac tgaagctgtt
     1021 atcaccgtac cggcatactt taacgatgct cagcgtcagg caaccaaaga cgcaggccgt
     1081 atcgctggtc tggaagtaaa acgtatcatc aacgaaccga ccgcagctgc gctggcttac
     1141 ggtctggaca aaggtactgg caaccgtact atcgcggttt atgacctagg tggtggtact
     1201 ttcgatattt ctattatcga aatcgacgaa gttgatggcg aaaaaacctt cgaagttctg
     1261 gctaccaacg gtgataccca cctgggtggt gaagacttcg acagccgtct gatcaactac
     1321 ctggttgaag aattcaagaa agatcagggc attgacctgc gcaacgatcc gctggcaatg
     1381 cagcgcctga aagaagcggc agaaaaagcg aaaatcgaac tgtcttccgc tcagcagacc
     1441 gacgttaacc tgccgtacat cactgcagat gcgaccggtc cgaaacacat gaacatcaaa
     1501 gtgactcgtg cgaaactgga aagcctggtt gaagatctgg taaaccgttc cattgagccg
     1561 ctgaaagttg cactgcagga cgctggcctg tccgtatctg atatcgacga cgttatcctc
     1621 gttggtggtc agactcgtat gccaatggtt cagaagaaag ttgctgagtt ctttggtaaa
     1681 gagccgcgta aagacgttaa cccggacgaa gctgtagcaa tcggtgctgc tgttcagggt
     1741 ggtgttctga ctggtgacgt gaaagacgta ctgctgctgg acgttacccc gctgtctctg
     1801 ggtatcgaaa ccatgggcgg tgtgatgacg acgctgatcg cgaaaaacac cactatcccg
     1861 accaagcaca gccaggtgtt ctctaccgct gaagacaacc agtctgcggt aaccatccat
     1921 gtgctgcagg gtgaacgtaa acgtgcggct gataacaaat ctctgggtca gttcaacctg
     1981 gatggtatca acccggcacc gcgcggcatg ccgcagatcg aagttacctt cgatatcgat
     2041 gctgacggta tcctgcacgt ttccgcgaaa gataaaaaca gcggtaaaga gcagaagatc
     2101 accatcaagg cttcttctgg tctgaacgaa gatgaaatcc agaaaatggt acgcgacgca
     2161 gaagctaacg ccgaagctga ccgtaagttt gaagagctgg tacagactcg caaccagggc
     2221 gaccatctgc tgcacagcac ccgtaagcag gttgaagaag caggcgacaa actgccggct
     2281 gacgacaaaa ctgctatcga gtctgcactg actgcactgg aaactgctct gaaaggtgaa
     2341 gacaaagccg ctatcgaagc gaaaatgcag gagctggcac aggtttccca gaaactgatg
     2401 gaaatcgccc agcagcaaca tgcccagcag cagactgccg gtgctgatgc ttctgcaaac
     2461 aacgcgaaag atgacgatgt tgtcgacgct gaatttgaag aagtcaaaga caaaaaataa
     2521 tcgccctata aacgggtaat tatactgaca cgggcgaagg ggaatttcct ctccgcccgt
     2581 gcattcatct aggggcaatt taaaaaagat ggctaagcaa gattattacg agattttagg
     2641 cgtttccaaa acagcggaag agcgtgaaat caaaaaggcc tacaaacgcc tggccatgaa
     2701 ataccacccg gaccgtaacc agggtgacaa agaggccgag gcgaaattta aagagatcaa
     2761 ggaagcttat gaagttctga ccgactcgca aaaacgtgcg gcatacgatc agtatggtca
     2821 tgctgcgttt gagcaaggcg gcatgggcgg cggcgcagac ttcagcgata tttttggtga
     2881 cgttttcggc gatatttttg gcggcggacg tggtcgtcaa cgtgcggcgc gcggtgctga
     2941 tttacgctat aacatggagc tcaccctcga agaagctgta cgtggcgtga ccaaagagat
     3001 ccgcattccg actctggaag agtgtgacgt ttgccacggt agcggtgcaa aaccaggtac
     3061 acagccgcag acctgtccga cctgtcatgg ttctggtcag gtgcagatgc gtcagggttt
     3121 ctttgccgtg cagcagacct gtccacactg tcagggccgc ggtacgctga tcaaagatcc
     3181 gtgcaacaaa tgtcatggtc atggtcgtgt tgagcgcagc aaaacgctgt ccgttaaaat
     3241 cccggcaggg gtggacactg gagaccgcat ccgtcttgcg ggcgaaggtg aagcgggcca
     3301 gcatggcgca ccggcaggcg atctgtacgt tcaggttcag gttaaacagc acccgatttt
     3361 cgagcgtgaa ggcaacaacc tgtattgcga agtcccgatc aacttcgcta tggcggcgct
     3421 gggtggcgaa atcgaagtac cgacccttga tggtcgcgtc aaactgaaag tgcctggcga
     3481 aacccagacc ggtaagcttt tccgtatgcg cggtaaaggc gtcaagtctg tccgcggtgg
     3541 cgcacagggt gatttgctgt gccgcgttgt cgtcgaaaca ccggtaggcc tgaacgagaa
     3601 gcagaaacag ctgctgcaag agctgcaaga aagcttcggt ggcccaaccg gcgagcacaa
     3661 cagcccgcgc tcaaagagct tctttgatgg tgtgaagaag ttttttgacg acctgacccg
     3721 ctaacctccc caaaagcctg cccgtgggca ggcctgggta aaaatagggt gcgttgaaga
     3781 tatgcgagca cctgtaaagt ggcggggatc actccccgcc gttgctctta ctcggattcg
     3841 taagccgtga aaacagcaac ctccgtctgg ccagttcgga tgtgaacctc acagaggtct
     3901 tttctcgtta ccagcgccgc cactacggcg gtgatacaga tgacgatcag ggcgacaatc
     3961 atcgccttat gctgcttcat tgctctcttc tccttgacct tacggtcagt aagaggcact
     4021 ctacatgtgt tcagcatata gggggcctcg ggttgatggt aaaatatcac tcggggcttt
     4081 tctctatctg ccgttcagct aatgcctgag acagacagcc tcaagcaccc gccgctatta
     4141 tatcgctctc tttaacccat tttgttttat cgattctaat cctgaagacg cctcgcattt
     4201 ttgtggcgta attttttaat gatttaatta tttaacttta atttatctct ttatcgcaat
     4261 tattgacgac aagctggatt atttttgaaa tattgtccta acaaataacg ccgactgaca
     4321 agaaattaat tattactttt cctaattaat ccctcagaaa tcctcacctt aagctatgat
     4381 tatctgagct tagggtcact cgt
//
LOCUS       DAJTNA010000259         4343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTNA010000259 DAJTNA010000000
VERSION     DAJTNA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.265"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            28..243
                     /locus_tag="OO161_003905"
     CDS             28..243
                     /locus_tag="OO161_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5464776.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            236..622
                     /locus_tag="OO161_003906"
     CDS             236..622
                     /locus_tag="OO161_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5464777.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            630..1316
                     /locus_tag="OO161_003907"
     CDS             630..1316
                     /locus_tag="OO161_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5464778.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="OO161_003908"
     CDS             complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="OO161_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5464779.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1999..3204
                     /gene="tet(B)"
                     /locus_tag="OO161_003909"
     CDS             1999..3204
                     /gene="tet(B)"
                     /locus_tag="OO161_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5464780.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="OO161_003910"
     CDS             complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="OO161_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5464781.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3998..>4343
                     /locus_tag="OO161_003911"
     CDS             3998..>4343
                     /locus_tag="OO161_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5464782.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     YQFDSQQSFTRRF"
BASE COUNT         1225 a          778 c          916 g         1424 t
ORIGIN      
        1 ccactattaa gtcatgtagc tggttttatt tcaattgaac gttttcaaag tctagctaca
       61 gaaggaaaaa tgttatcgct atcttggtgg gaaaacgaat acgcagttct gcaatggaaa
      121 aatcatgttt tacatgcgaa agctcaacaa gaagggcgag agtcaatatt tgatttttac
      181 aaaattagta ttgctcatat tactcgcgaa tattcattta aaaaggacaa ggataatgtt
      241 tgatgttcac gttgttttag ataatcaaat aggacaatta gcattactag gaaaaacatt
      301 aggtaataaa ggtattggat tggaaggggg agggatattt acggttggtg atgaatgcca
      361 tgctcatttt cttgttgaac aaggaaagga agctaaaata gcgctagagc aagctggact
      421 gttagtactt gcgatccgga caccattaat tcgtaagtta aaacaggaaa aaccggggga
      481 acttggcgaa atagcacgag tattggcgga gaataacatt aatattttag tgcaatacag
      541 tgaccatgct aaccaactga tattaataac ggacaatgat agtatggctg catctgttac
      601 gctcccttgg gcaataaagt gaacttgcga tggctaattt aatacgaaaa gaggttacct
      661 ttgagtcctc aatagccgcg ataggggcgg ctatgtctga catttcacga gttaaaatac
      721 tcagtgcttt gatggatggg cgagcttgga cggccactga gctaagttct gtggcgaata
      781 tatcagcttc aacggcgagc agtcatttat ctaaattatt agattgccag ctaatcacag
      841 tagtagctca aggcaagcat cgttattttc ggctagcagg aaaagatatt gctgaattga
      901 tggaaagtat gatggggatc tccttaaacc atggcgtaca tgccaaagtt tccacgccag
      961 tgcatttacg aaaagcacgt acttgctatg atcatttagc tggcgaagtt gccgttaaga
     1021 tctatgattc cctttgtcaa cagcaatgga tcactgaaaa tggttcaatg atcacattaa
     1081 gtggtattca atattttcat gaaatgggaa ttgacgttcc ttccaaacat tcacgtaaaa
     1141 tctgttgtgc gtgtttagat tggagtgaac gccgtttcca tttaggtggg tacgttggag
     1201 ccgcattatt ttcgctttat gaatctaaag ggtggttaac tcgacatctt ggttaccgtg
     1261 aagttaccat cacggaaaaa ggttatgctg cttttaagac ccactttcac atttaagttg
     1321 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
     1381 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
     1441 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
     1501 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
     1561 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
     1621 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
     1681 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
     1741 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
     1801 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
     1861 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
     1921 taattcctaa tttttgttga cactctatca ttgatagagt tattttacca ctccctatca
     1981 gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat tacgttactc
     2041 gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg tgaatttatt
     2101 gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc gttaatgcag
     2161 gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg
     2221 ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc aagtgcgctt
     2281 tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg ggctgtcgcg
     2341 gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt cggttggtta
     2401 ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt tgcaggagag
     2461 atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc tttccttgtg
     2521 gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac cgaagtaggg
     2581 gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc cattttgttg
     2641 attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg ggtgctattt
     2701 accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc gggtcttggt
     2761 cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa atggggcgaa
     2821 aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt tttagcgttt
     2881 atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg tgggatcgct
     2941 ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca aggtgcttta
     3001 cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt actgtttgct
     3061 gttatttata atcattcact accaatttgg gatggctgga tttggattat tggtttagcg
     3121 ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca agctcagggg
     3181 agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata
     3241 taatgaccct cttgataacc caagagggca ttttttacga taaagaagat ttagcttcaa
     3301 ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg taaatagcaa
     3361 taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt gcgatatgca
     3421 taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta agttggttct
     3481 cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca aggctaattt
     3541 tttcaagttc attccaacca atgataggca tcacttcttg gatagggata aggtttttat
     3601 tattatcaat aatataatca agataatgtt caaatatact ttctaaggca gaccaaccat
     3661 ttgttaaatc agtttttgtt gtgatgtagg catcaatcat aattaattgc tgcttataac
     3721 aggcactgag taattgtttt ttatttttaa agtgatgata aaaggcacct ttggtcacca
     3781 acgcttttcc cgagatctca tctattgaaa cagcttgata gcctttttca acaaacaata
     3841 ttcgtgctga gttaaccagt gattgatagg tactcttaaa attttcttgt tgatgatttt
     3901 tattttccat gatagattta aaataacata ccgtcagtat gtttatggta tcatgatgat
     3961 gtggtcgtga caatcttaag aacatttagg ttattttatg tatattgaac agcattctcg
     4021 ctatcaaaat aaagctaata acatccaatt aagatatgat gataagcagt ttcatacaac
     4081 ggttatcaaa gatgttctat tatggattga acataattta gatcagtctt tactgcttga
     4141 tgatgtggcg aataaagcgg gttataccaa gtggtatttt cagcggctgt tcaaaaaagt
     4201 aacaggggtc acactggcta gctatattcg tgctcgtcgt ttgacgaaag cggctgttga
     4261 gttgaggttg acgaaaaaaa ctatccttga gatcgcatta aaatatcaat ttgattccca
     4321 acaatctttt acacgtcgat tta
//
LOCUS       DAJTNA010000260         4324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTNA010000260 DAJTNA010000000
VERSION     DAJTNA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.266"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..212)
                     /locus_tag="OO161_003912"
     CDS             complement(<1..212)
                     /locus_tag="OO161_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5464783.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPV"
     gene            521..2293
                     /gene="aspS"
                     /locus_tag="OO161_003913"
     CDS             521..2293
                     /gene="aspS"
                     /locus_tag="OO161_003913"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5464784.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2411..2863
                     /gene="nudB"
                     /locus_tag="OO161_003914"
     CDS             2411..2863
                     /gene="nudB"
                     /locus_tag="OO161_003914"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5464785.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2892..3632
                     /locus_tag="OO161_003915"
     CDS             2892..3632
                     /locus_tag="OO161_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5464786.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3667..4188
                     /gene="ruvC"
                     /locus_tag="OO161_003916"
     CDS             3667..4188
                     /gene="ruvC"
                     /locus_tag="OO161_003916"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5464787.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLC"
BASE COUNT         1071 a         1075 c         1212 g          966 t
ORIGIN      
        1 tcaaccggct gttttaatgc ttcggcgtaa tcggcagacc agccaactcg caccagaaac
       61 acgggctgac cgctggcgcg aaatttcgcc gccagcttcc cggcgcgatt aactacctca
      121 tcggcagtat gtggaccacc ggcaaaaggt aagatgcctt cttgtaaatc gatcaccacc
      181 aacgcagtgg ttttagcatt aagttcaagc atgctgactc ctgaaaaaac aaaatcgtgc
      241 ctcacacctt aatgcgatta gccgatggcg gaacgacaaa ttttgttaat ttttatgagt
      301 tcgggcaaca cctttatagc aaactggcgt agagtctgat ttcaggcccg atataaagtg
      361 gtaacgaggc gttccagcgc gggccaggct cttacggtgc ggcagaattt ccagtataat
      421 agccgccttt tttcattcag ttgtgacata cagctaacgc tgcgacttgg tcacctgcgc
      481 agcaggggca tccgccggca gctaagttaa gggatatctc atgcgtacag aatattgtgg
      541 acagctccgt ttgtcccacg tggggcagca ggtgactctg tgtggttggg tcaaccgtcg
      601 tcgtgatctt ggtagcctga tcttcatcga tatgcgcgac cgcgaaggta tcgtgcaggt
      661 atttttcgat ccggatcgtg cggacgcgtt aaagctggcc tctgaactgc gtaatgagtt
      721 ctgcattcag gtcacgggca ccgtacgtgc gcgtgacgaa aaaaatatta accgcgatat
      781 ggcgaccggc gaaatcgaag tgctggcgtc ctcgctgact atcatcaacc gcgcagatgt
      841 tctgccgctt gactctaacc acgtcaacac cgaagaagcg cgtctgaaat accgctacct
      901 cgacctgcgt cgtccggaaa tggctcagcg cctgaaaacc cgcgctaaaa tcaccagcct
      961 ggtgcgccgt tttatggatg accacggctt cctcgacatc gaaactccga tgctgaccaa
     1021 agccacgccg gaaggcgcgc gtgactacct ggtgccttct cgtgtgcaca aaggtaaatt
     1081 ctacgcactg ccgcaatccc cacagttgtt caaacagctg ctgatgatgt ccggttttga
     1141 ccgttactat cagatcgtta aatgcttccg tgacgaagac ctgcgcgctg atcgtcagcc
     1201 tgagtttact cagatcgatg tggaaacttc tttcatgacc gcgccgcaag tgcgtgaagt
     1261 gatggaagcg ctggtgcgtc atttgtggct ggaagtgaag ggcgtggatc tgggcgattt
     1321 cccggtaatg acctttgcgg aagcagaacg ccgttatggt tctgataaac cggatctgcg
     1381 taacccgatg gaactgaccg acgttgccga tctgctgaaa tctgttgagt ttgcagtatt
     1441 tgcggggccg gcgaacgatc cgaaaggccg tgttgccgct ctacgtgttc cgggtggcgc
     1501 atcgctgacc cgtaagcaga tcgacgaata cggtaacttc gttaaaatct acggcgcgaa
     1561 aggtctggct tacatcaaag ttaacgaacg cgcgaaaggt ctggaaggta tcaacagccc
     1621 ggtagcgaag ttccttaatg cagaaatcat cgaagccatc ctggatcgta ctgccgcgca
     1681 agatggcgat atgattttct tcggtgccga caacaagaaa attgttgccg acgcgatggg
     1741 cgcactgcgc ctgaaagtgg gtaaagacct tggtctgacc gacgaaagca aatgggcacc
     1801 gctgtgggtt atcgacttcc cgatgtttga agacgacggt gaaggcggcc tgacggcaat
     1861 gcaccatccg ttcacctcac cgaaagacat gactgctgca gaactgaaag ctgcaccgga
     1921 aaatgcggtg gcgaacgctt acgatatggt catcaatggt tacgaagtgg gcggtggttc
     1981 agtacgtatc cataatggtg atatgcagca gacggtgttt ggtattctgg gtatcaacga
     2041 agaggaacag cgcgagaaat tcggcttcct gctcgacgcc ctgaaatacg gtactccgcc
     2101 gcacgcaggt ctggcattcg gtcttgaccg tctgaccatg ctgctgactg gcaccgacaa
     2161 tatccgtgac gttatcgcct tcccgaaaac cacggcggca gcgtgtctga tgactgaagc
     2221 accgagcttt gctaacccga ctgcactggc tgagctgagc attcaggttg tgaagaaggc
     2281 agagaataac tgatatgatt caaatacacg aaatcattcg cgttgcatca aggcggcaat
     2341 tgagtgaact cccatgagca tagataacta tgtgaatggg atgagcgaag gcagtcaacg
     2401 aagaggcagc gtgaaggata aagtgtataa gcgtcccgtt tcgatcttag tggtcatcta
     2461 tgcacaagat acgaaacggg tgctgatgtt gcagcggcgt gacgatcccg atttctggca
     2521 gtcggtaacc ggcagcgtgg aagagggtga aaccgcgccg caagctgcca tgcgcgaagt
     2581 aaaggaagag gtcaccattg atgttgtcgc tgaacaactg accttaattg actgtcagcg
     2641 cacggtagag tttgaaattt tttcacattt acgtcatcgc tatgcgccgg gcgtgacgcg
     2701 taatacggaa tcatggttct gtcttgcgct tccgcacgag cggcagatcg ttttcactga
     2761 acatctggct tacaagtggc ttgatgcgcc tgctgcggcg gcgctcacta agtcctggag
     2821 caaccggcag gcgattgaac agtttgtaat taacgctgcc tgaacaggca gggctatctg
     2881 gagatatttt tatggcaggt catagtaaat gggccaacac cagacatcgt aaagctgcgc
     2941 aggatgctaa gcgcggtaaa atcttcacta aaatcattcg tgagctggta accgcggcta
     3001 agctgggcgg tggcgatccg gacgctaacc cgcgtctgcg tgcggcggtg gataaagcac
     3061 tgtctaacaa catgacccgt gacacactga accgcgcaat tgcacgtggt gtgggcggtg
     3121 atgatgatgc aaacatggaa accatcatct acgaaggtta cggtcctggc ggcacggcaa
     3181 tcatgattga atgtctgtct gacaaccgca accgtaccgt tgctgaagtg cgtcatgcat
     3241 tcagcaaatg tggcggtaac ctcggtacag atggttccgt agcctatctg ttcagcaaaa
     3301 aaggcgtgat ctccttcgag aaaggcgatg aagacaccat catggaagca gctctggaag
     3361 caggtgctga agacgttgtg acctatgatg acggcgcgat tgatgtctac actgcatggg
     3421 aagaaatggg taaagtgcgc gacgctctgg aagcggcagg tctgaaagca gacagcgcgg
     3481 aagtttccat gatcccgtcc accaaagctg atatggatgc agaaaccgca ccgaaactga
     3541 tgcgtctgat cgatatgctg gaagattgcg acgacgtgca ggaagtttac cataacggtg
     3601 aaatctctga tgagattgca gcgactctct gatgaggcct gctaaacagc aaaacggaga
     3661 cgcgtgatgg ctattattct cggcattgac ccgggttcgc gcgtgaccgg ctatggcgtc
     3721 atccgccagg taggcaggca actgtcctac ctgggtagcg gatgcatacg caccaaggtg
     3781 gatgatttac cgtctcgtct gaagctcatc tatgcgggcg tgacggaaat catcacccag
     3841 ttccagccag attatttcgc cattgaacaa gtctttatgg ccaaaaatgc cgactcggct
     3901 ctgaaactgg ggcaggcgcg cggcgtggcg attgtggcgg cggtgaatca ggaattgccg
     3961 gtatttgaat atgcggcacg tcaggtaaag caaacggtgg taggtattgg tagtgccgaa
     4021 aaaagtcagg tgcagcatat ggtccgcacc ttgctgaaac tgcccgctaa tccacaggcg
     4081 gatgccgccg atgcgttagc tatcgcgatt acccattgtc acgtcagcca gaatgcaatg
     4141 cagatgagcg aatcgcggct gaacctggcg agagggcgac tgtgttaagt tataccgcct
     4201 cggtcagttc aggctgaggc gtttcaactc cctccgcgag aatcaacacc aaacccggat
     4261 gatgccacaa actggctggc gtgacggttt tacgctccca cggaatagaa cctaagaacc
     4321 agaa
//
LOCUS       DAJTNA010000261         4295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTNA010000261 DAJTNA010000000
VERSION     DAJTNA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.267"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            47..2209
                     /gene="ybiO"
                     /locus_tag="OO161_003917"
     CDS             47..2209
                     /gene="ybiO"
                     /locus_tag="OO161_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR5464788.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2269..3195)
                     /gene="rlmF"
                     /locus_tag="OO161_003918"
     CDS             complement(2269..3195)
                     /gene="rlmF"
                     /locus_tag="OO161_003918"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5464789.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3471..3731
                     /locus_tag="OO161_003919"
     CDS             3471..3731
                     /locus_tag="OO161_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5464790.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            3996..>4295
                     /locus_tag="OO161_003920"
     CDS             3996..>4295
                     /locus_tag="OO161_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5464791.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT          996 a         1133 c         1077 g         1089 t
ORIGIN      
        1 agcatctata cttatctttt tgctctgttt tctcactgga ggagtcatgc ggtggatcct
       61 gttcatcctc ttctgcctgc tgggcgcacc tgcccacgcg gtatccatac ccggcgttac
      121 aaccacaacg acaacggact caacgactga accggccccg gaaccggata tcgaacaaaa
      181 aaaagcggcc tatggcgcac tggcggatgt gctggataac gacacctcgc gtaaagaatt
      241 gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg gaaccggtcc cgaagatcgt
      301 gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag gtcaccgaag tcagccgcca
      361 ttatggtgaa gccctttccg cccgcttcgg gcaactttat cgcaatatta ccggctcccc
      421 gcataagccg tttaatccac aaacattcag caatgcgctg acccattttt caatgttagc
      481 ggtattagtg tttggttttt actggctgat tcgcctgtgc gcactgccgc tgtatcgcaa
      541 aatgggcctg tgggcgcggc aaaaaaatcg tgagcgcagt aactggttgc agcttccggc
      601 gatgattatc ggggcgttta ttatcgacct actgttactg gcactgacat tatttgtcgg
      661 ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc atcgctttcc aacaaagttt
      721 gtttctcaac gcctttgccc tgattgaatt tttcaaagct gtactacgcc tgattttttg
      781 cccaaacgtg gctgaactgc gcccgttcac gattcatgac gagaccgccc gttactggag
      841 tcgtcgcctg agctggttaa gcagtttgat aggttatggc ctgattgtgg ccgtgccgat
      901 tatctctaat caggtgaatg tacagatagg tgcgctggcg aacgtcatca ttatgctgtg
      961 catgaccgtc tgggcgttgt acctgatctt tcgcaataaa aaagagatta cccaacattt
     1021 gctcaacttt gcggagcatt cgctggcctt tttcagcctg tttatccgcg cctttgcgct
     1081 ggtgtggcac tggctggcaa gcgcctattt tatcgtgctg tttttctttt cgttgttcga
     1141 tccgggcaac agcctgaaat ttatgatggg tgcaacggtg cgcagcctgg cgattattgg
     1201 tatcgcagcg tttgtttccg gtatgttttc ccgctggctg gcgaaaacca tcaccctctc
     1261 gccacatact cagcgtaact atccggagtt gcaaaaacgg ctgaatggct ggctttcggc
     1321 ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggcg gtaatgttgc tgttgagcgc
     1381 atggggattg ttcgatttct ggaactggct gcaaaacggc gcggggcaga aaaccgtaga
     1441 tatcctgatc cgtatcgcac ttattctttt tttctcggcg gttggctgga cggtgctcgc
     1501 cagtttgatc gaaaaccggc tggcttcgga tattcatggc cgcccgctac ccagcgcccg
     1561 tacgcgtacc ctgctgacgc tgtttcgtaa cgcgctggcg gtgattatca gtaccatcac
     1621 catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg ccattgctgg caggtgccgg
     1681 ggcattaggt ctggctatct cgtttggttc gcaaacgctg gtgaaagata tcattaccgg
     1741 ggtgtttatt cagtttgaaa acggcatgaa cactggagat ttggtgacta tcgggccgtt
     1801 gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc gtgcgccagg ataccggggc
     1861 gtatcacatc attccgtggt cttcgataac cacctttgct aacttcgtcc gcggtattgg
     1921 ttcggtagtg gcaaactacg atgttgatcg ccatgaagat gctgataaag ccaatcaggc
     1981 actgaaagat gcggtagcgg aattgatgga aaacgaagaa attcgcgggc tgattattgg
     2041 tgaaccgaat tttgccggga ttgtcggctt aagcaatacc gcgtttacac tgcgtgtttc
     2101 gttcaccacg ctgccactca aacagtggac ggtacgcttt gccctcgaca gccaggtgaa
     2161 aaaacatttc gacctggtgg gcgttcgcgc gccagtgcag acttattagg tgctgcctac
     2221 tccgggcgcg accccggctg aaccgttacc gccgggggag ccaacgcttt aacgctggcg
     2281 attgacaaaa cggcggcgct gctcgtcgtt cataaaggtc caggcaataa agcgactctg
     2341 cttttgcccc tgggccatct ctttttttac caccttcacc gcgcccacgt cggtcagagc
     2401 acgatacaac ggcggtaagt tttcaccacg agataccagc gaggtaaacc acatcacctg
     2461 cttcgcgaag cctttgctct cttcaatcat ctttttgata aaggcgactt caccaccttc
     2521 acaccacaac tcctgttgct gaccaccaaa gttcagtgca tcgtctttgt tcagccccag
     2581 gttacggcgt ttacgctcac tacctgcccg cgctgcagcg gcggaatcgt ggaatggcgg
     2641 gttacacaag gtcgcgtcgt attgctcgtt tttatggatg atgccgttaa aaattgcccc
     2701 actctctttt tgccgacgca gacgaatggc gcggttaagc cccggattag cactgataat
     2761 cgcctgcgca ctgcttaacg cctggctgct ggtttcacta ccggtaaaac gccagccata
     2821 ttcatgtacg ccaatcagcg gataaataca gttcgcacca acgccgatat ccagaatgct
     2881 ggcattcgct ggaattgttc cgcttgcttc agccagtaaa tcggcaaggt gatgaatata
     2941 atccgcccgg cccggtactg gtgggcagag aaaaccgtcg gggatatccc agttcgctac
     3001 ggcgtaaaaa tgggccagca acgccttatt gagcgccttc accgccagcg gattggcaaa
     3061 gtctacgctt tgctccccgg cgggtgtaag cgtgaggaat tgcctgagtt caggattgac
     3121 ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg ttgcgcggat gcaaccccgg
     3181 tttctgggcg gacatagcat tctcctttaa gcagcggcgt aagatacccg cataacgcga
     3241 cagggtaaat atctggctgc gtttatcatt ccccaaaacc ttaacatggc tatggtttat
     3301 ggcaattcat cataaaaatc agcattatgc atgtcattga tttgtcttat tttgtaaaaa
     3361 tatatcgttc aaaaaataag cacatctctg tcaatttttt gcgtcatttg catcgcgcag
     3421 gcgacctaca cttatcaggc actacccgtt gtatttatga ggatgtcatt atgaaaaagt
     3481 gcctcacact actgattgcc accgtcctga gcggaatctc tctcacggct tatgccgcgc
     3541 aaccgatgag taacctggac agcggtcagc tacggcccgc cggtaccgtt tcggcaactg
     3601 gcgcatcaaa cctaagcgat ctggaggata aactggcaga aaaagcgcgc gaacaaggcg
     3661 cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca gatgttcggt actgcaacca
     3721 tctacaaata accccaccaa cgtaactgca ttcgcctctg gatgcagttt tcgcctgttt
     3781 tgtaatcaaa aaacatcaaa tatgaactca atgtaaataa atgtatttct ttttcgcgca
     3841 atgggtgata gaaaatcgcg ccaagtgata atgcttatca gaattattat cacttgcacg
     3901 aacactatca cgggattaac agtggcatcg catccgcaga gatgctttct cgtggcagtg
     3961 aaaatttcaa catataagaa aaagtcacct gcaaaatgga aaacaatcgc aatttccctg
     4021 ccagacaatt tcattcgctc acgttctttg ccggtctttg tattggcatc acacctgtgg
     4081 ctcaggcact cgccgccgaa gggcaaacta acgcggatga cacgctggtt gtcgaagcat
     4141 caacgccttc gctttatgcg ccacaacaat ctgccgatcc gaaattctcg cgtccggtag
     4201 cggatactac ccgcacgatg acggtgattt ctgaacaagt gattaaagat cagggcgcaa
     4261 ccaaccttac cgacgcgctc aaaaacgtcc ccggc
//
LOCUS       DAJTNA010000262         4217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTNA010000262 DAJTNA010000000
VERSION     DAJTNA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.268"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO161_003921"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO161_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5464792.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO161_003922"
     tRNA            complement(324..399)
                     /locus_tag="OO161_003922"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO161_003923"
     tRNA            complement(406..480)
                     /locus_tag="OO161_003923"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO161_003924"
     tRNA            complement(597..681)
                     /locus_tag="OO161_003924"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO161_003925"
     tRNA            complement(690..765)
                     /locus_tag="OO161_003925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO161_003926"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO161_003926"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5464793.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO161_003927"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO161_003927"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5464794.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO161_003928"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO161_003928"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5464795.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1088 a         1052 c          941 g         1136 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgttgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caagcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccca
     4201 gtcttccgac cgggcct
//
LOCUS       DAJTNA010000263         4204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTNA010000263 DAJTNA010000000
VERSION     DAJTNA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.269"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            113..616
                     /gene="yeaK"
                     /locus_tag="OO161_003929"
     CDS             113..616
                     /gene="yeaK"
                     /locus_tag="OO161_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5464796.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(617..721)
                     /locus_tag="OO161_003930"
     CDS             complement(617..721)
                     /locus_tag="OO161_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464797.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            890..1336
                     /locus_tag="OO161_003931"
     CDS             890..1336
                     /locus_tag="OO161_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5464798.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEQQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1293..2114)
                     /gene="nimR"
                     /locus_tag="OO161_003932"
     CDS             complement(1293..2114)
                     /gene="nimR"
                     /locus_tag="OO161_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR5464799.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNHARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2211..3407
                     /gene="nimT"
                     /locus_tag="OO161_003933"
     CDS             2211..3407
                     /gene="nimT"
                     /locus_tag="OO161_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR5464800.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDLPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGTVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            3447..3794
                     /locus_tag="OO161_003934"
     CDS             3447..3794
                     /locus_tag="OO161_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5464801.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHDQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3816..4070)
                     /gene="yoaF"
                     /locus_tag="OO161_003935"
     CDS             complement(3816..4070)
                     /gene="yoaF"
                     /locus_tag="OO161_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5464802.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
BASE COUNT          969 a         1067 c         1038 g         1130 t
ORIGIN      
        1 acatgatgcg tataaagctt ccgatgagcg tttatatgtc aataagcaga acaaaaacag
       61 ccgttcatga taaccttctg tggttgtttg cttgtaatct caggagcgtg aaatgactga
      121 aatggctaaa ggaagcgtga cgcatcagcg gttaatcgct ttattatcac aagaaggtgc
      181 taacttccgc gtcgttaccc atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg
      241 tggcaccgct ttaggccagg gagctaaggc actggtatgt aaagtcaaag gcaatggcgt
      301 aaatcaacat gttctggcga tcctcgctgc cgatcaacag gccgatctga gccaacttgc
      361 cagccatata ggcggattac gcgcttcgtt agccagccct gctgaagtcg atgaactcac
      421 aggctgtgtc ttcggcgcga tccccccttt cagcttccat ccaaaactca aactggttgc
      481 cgaccctcta ctcttcgaac gatttgatga aatcgccttc aatgcaggga tgctggataa
      541 atccgttatt ttgaaaacca cggattattt gcgcattgcg caaccagaac tggtcaattt
      601 ccgccgcact gcgtaactag cctgtccgtt caataagcag aacggacaaa accagcacaa
      661 cagcaatggc aaaaaacgat gacgtgataa tcagtgtctc gacgaacatt tgatcgttca
      721 tagcatgccc ccgggagata gctgttttca cgttattgtt agcgtgcaca aatggcagtt
      781 tgatgacagt tcgcctgatt tttatgcaaa aaacgtaaag attttctact tccttcctgc
      841 agcaagcgta aagtaagcag gcttattatt ttttggcaag gaaaccacga tgtttgatgt
      901 cactttgctg atcctgctcg ggttagctgc gctaggcttt atcagtcata acaccactgt
      961 cgccgtttca attctggtgt tgatcattgt ccgcgtcaca ccgttaagca cctttttccc
     1021 gtggattgaa caacaggggc taagtatcgg gataatcatt ctgacgattg gcgttatggc
     1081 ccccatcgcc agcgggacgc taccgccctc aaccttgatt cactcgtttc tgaactggaa
     1141 atcgctggtg gcaattgcag taggcgtgat tgtctcctgg ctgggtgggc gcggcgtgac
     1201 cttaatgggc agccagccgc aactagtcgc cgggctgctg gtaggcactg ttttaggcgt
     1261 ggcgctgttt cgcggcgtac cggtcggccc gcttattgcc gcgggtctgg tttcgctgat
     1321 tgtggggaaa cagtagttaa tcccgcaata tatcggcctg gcgtttgccc caggcctttc
     1381 ttaaacatag taataaaggc cgttgttgaa tcatacccca gcgtatgagc aactttctgt
     1441 acagtatcgc ctttcactaa tccctgaagt gccataatca gctgtaactg ctgacgccac
     1501 tggcgaaaac tcaaccccgt ctcttttact atcagtcgcg catgattgcg ttcactcatc
     1561 gcaaaaaagc cagcccattg ccctaatgac ccccactcga cgggcccttt cgccatcatc
     1621 tccaccatcg tgcggatttt aggatgagaa gaaacgggca ggtgcagctg ttgttgtggc
     1681 tgctgaggga gttcatcaaa caggacctgt attaatctgc gggtcatcgg ctcagcacgc
     1741 tgagtcgtcg tcctgtttgc cagggttaaa atcaattccc ggcataaggg cgatattttc
     1801 aatgtgcaac atgttgtcgg cattgtcact gcgctgggtt cgataaacag aaaacagagt
     1861 tcagcgttag ctgtcacttg attgctatgt tccaccccgc ccggtatcca aaccgcatat
     1921 tgcggcggca ccatccacaa agcattttcc accgtacagg taattgcacc atgtaatgct
     1981 agtatcagct gtcctttgcg gtgctgatgt acaggactgg ttaactcatc cgttccggcg
     2041 tgaatacaaa atgcgaccgc ggcttcatgg tgtcggtcgg gttcatagcc attgagattc
     2101 aacctgtgca tcattttgtc cgaatttagc gataatttgt cattttagct tgattcaaca
     2161 taacaataaa aacggtaagg tacagcctcg tttgtaacaa tgagaagcat atgacctgtt
     2221 caacttcatt aagcggcaaa aacaggattg tccttatcgc tggcattctg atgattgcca
     2281 caacattacg cgtcaccttt accggcgcag caccgttact ggatacgatt cgttccgctt
     2341 actcgctgac gacagcgcaa accggcttat tgaccaccct gccattattg gcctttgcgc
     2401 taatctcacc tttggccgcc ccggtagcgc gacgttttgg tatggaacgt agcctgtttg
     2461 ccgcgttact tttgatctgt gctggtatcg caattcgctc tctcccttcg ccttacttat
     2521 tatttggcgg tacagcggtc attggcggtg ggattgcatt aggcaatgtc ttactgccag
     2581 gattaattaa acgcgatctc cctcattccg tcgccagact taccggcgca tattccctga
     2641 caatgggagc tgcggcggca cttggatcgg ctatggtagt gccgctggct ttgaacggtt
     2701 ttggctggca aggcgcgttg ctcatgctga tgtgttttcc tctgctggct ctttttttat
     2761 ggctgccaca gtggcgaagc caacaacatg caaatttgag tacctcgcgc gccttacata
     2821 ctcggggtat ctggcgttcg ccgcttgcct ggcaggtcac attgtttctt gggagcaact
     2881 cactggtcta ttacgtgatt attggctggc ttccggcgat cctcatcagt cacggctata
     2941 gcgaagcaca ggcgggttca ctgcatggtt tgctgcaact agccacagca gctcccggtt
     3001 tgctgatccc tcttttctta catcatgtga aagaccagcg tggtattgca gcgttcgttg
     3061 ccttgatgtg cgcagtgggc actgttgggc tctgctttat gccagcgcac gcgatcacct
     3121 ggactctgct tttcggtttt ggttccggcg caacaatgat actggggttg acgttcattg
     3181 gtcttcgggc aagttctgcg catcaggcgg cggcactctc ggggatggca caatccgtcg
     3241 ggtatttgct ggcagcctgt gggccgccgc taatgggtaa aatacacgat gctaacggta
     3301 actggtccgt accactgatg ggtgttgcaa tactttcact actgatggcg attttcggac
     3361 tttgcgccgg gagagacaaa gaaattcgct catatccggt gctatagtga cgtgacaaat
     3421 catgcgtgaa agggagaaca aacacgatga atattcagtg caaacgcgtt tatgatccgg
     3481 ctgaacagag cgatggttat cgcgtactgg tcgaccgcct ctggccgcgc ggtatcaaaa
     3541 aaaccgattt agcccttgat gagtgggata aagaaatcac gccgtcaacg gaactgcgca
     3601 aagcctttca cggcgaagtc gtcgatttta caacctttcg cgagcaatat cttgcagaac
     3661 tggcgcaaca cgatcaagaa ggaaagcggc tggcggacat cgccaaaaaa cagccgctga
     3721 ccctgctcta cgcagcaaaa aacaccacgc agaaccatgc gctggtgctg gccgactggc
     3781 tacgtagctt gtgattttag tacagcatcc ggcggttatt tttcaccagc cggatggtcc
     3841 cgtcgccaca atgcccattc atcaatcgtt tcaccgcccg gtaatttgca attgttgctg
     3901 accccttgcg ctgtcgacac tggaatgagc gtcccgccct tctgctggca atagaccgac
     3961 gccggatttg ccataccaat ctgcggcggt ttaggtgctt ctggctgaga aggggttgaa
     4021 caaccagcca ggaccagcaa gcaaggcaga acaaaactga taattttcat ttattgatct
     4081 cacatattta tccagactta gagtatcgcg gtatcgtttt gttttgcagc actattttta
     4141 ttacattcac tcaaaacata ttacgtcttg tttcatcttt gttgatgatg ttttatcatg
     4201 cctg
//
LOCUS       DAJTNA010000264         4127 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTNA010000264 DAJTNA010000000
VERSION     DAJTNA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.270"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..438
                     /gene="tnpA"
                     /locus_tag="OO161_003936"
                     /pseudo
     CDS             1..438
                     /gene="tnpA"
                     /locus_tag="OO161_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(556..1500)
                     /gene="hypT"
                     /locus_tag="OO161_003937"
                     /gene_synonym="qseD"
     CDS             complement(556..1500)
                     /gene="hypT"
                     /locus_tag="OO161_003937"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5464803.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1845..2564
                     /locus_tag="OO161_003938"
     CDS             1845..2564
                     /locus_tag="OO161_003938"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5464804.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2630..3928
                     /locus_tag="OO161_003939"
     CDS             2630..3928
                     /locus_tag="OO161_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR5464805.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1042 a          896 c         1017 g         1172 t
ORIGIN      
        1 cgcacacccg atggaactgt aaatatcaca tagttttttg cgccaaaata ccgaagacag
       61 gtgttctaca gagagaagcg tagagcaaca ggcagtattt tgagaaagct gtgtgagtgg
      121 aaaagcgtac ggattctgga agctgaatgc tgtgcagatc atatccatat gcttgtggag
      181 atcccgccca aaatgagcgt atccggcttt atgggatatc tgaaagggaa aagcagtctg
      241 atgctttacg agcagtttgg tgatttgaaa ttcaaataca ggaacaggga gttctggtgc
      301 aggggggact acgtcgatac agtgggtaag aacacggcga agatacagga ttacataaag
      361 caccagcttg aagaggataa aatgggagag cagttatcga ttccctgtcc gggcagcccg
      421 tttacggacc gtaagtaacg aagttggatg caaatgtcag atcgtgtgcg cctgttaggg
      481 cgcggctggt aagagagcct tataggcgca tttgaaaaac ctccggctat gccggaggat
      541 atttattgca cgtcgtcaat tgcggcgtgc tggctcttcc attgatatct gctggattaa
      601 atcgtctggc atatgttcct gtaaaatacg ccagaggttc tcggcggttc tattcagtcg
      661 ggtgttcatt cggtaaatat aagcctgaat ggggactgtc aatttagcag catcaaggca
      721 tatcagacgt ttggtctgta gttcatcgac gatcgaccag atcggtaacc aggcaatgcc
      781 gtatccgttg agcgccatat tctttaataa ctcgctcatc gaagagataa atatcgtgcg
      841 cccggtaata cctccaacct ctgccagatg acgattgacc aacctgccca tataagatgt
      901 ggcggtatag ttgagcaaag gaacctgggg ctgagtgata tcgaatacag ggtgcccctg
      961 tgcgtcggcg gcacataccg ggtacaactc agattcaaac agtttcaggc agcaaaaagg
     1021 cgattgcata agatcttcat cgcgaaacga aataataaag tcactcttgc cttcgcgtaa
     1081 cgtatttacg gcctggacaa catcaatggc ttcaacatga tagacaaatt ctccgccata
     1141 ggcggtaagt gaatgcacca gtttgggtaa tacgctcagg gatagtgagt gcgcggctgc
     1201 aattttaatg ttgggaacgc cgagtaaact ctggccgttt aactccccca aattgcattc
     1261 aagctgctgt aataagctgc gagtctggga atgaaagagc ttgccctctt cggttaattg
     1321 taaaggggtg gtcgtgcgat cgaataaaac aacgccgatc gctgcctcca gcgccttaat
     1381 tcgtcggctg aatgcgggtt gcgaaagatt acgctctttt gccgcctgag aaaaatggcg
     1441 acaggcttcc agtgtcagga aatcgtataa ccattttgtc tcgatattct tcattaccat
     1501 ggcacctgta ttttcaatcc aaagacataa taaaacatcc cggcaataaa aataatgttc
     1561 tcaattagca caacctggcc tttatcattc gttttgtgca taaccccttc atttccggta
     1621 ttgacgttga tatcacattt cgccaaaaaa acgaaatttt tccgtcagat aaccagaata
     1681 catcatcact atggtgaata agcggaatgt tccatagctg catcatgcgt gtcttgcatc
     1741 gccttgtaca accttgcatt tctcatcgcc catttcgact gttaaaaaag agagcagcag
     1801 ttatttaccg cgtattgtca ttaacgtttt gagaggcagg tattgtgaaa ggtttaattg
     1861 gtgtactcgg tggaatgggg ccggcagcta ccgtcgacct gtttaataaa tttgtaactt
     1921 ttaccgctgc acaatgtgat caggaacata ttcctttaat tatttcttcg atcccggata
     1981 ttcctgaccg taccgatgca ttaatgcatc atgggcgttc accattaccc gttatgcgtg
     2041 attatatgca taagctggaa gatgccgggg cagaatgtat tgttattccc tgtaataccg
     2101 cgcattactg gttcaaagaa ttaaaagatg cttgtcatat tgatatatta agtatcgtag
     2161 aaactacgat caatgaagtt cgcgcctgtg gtaaaacccg aattggcttg ttagccacca
     2221 atgccacgct atatatgggt ttataccaaa aaggaattga gtcgctggga tttacctgtg
     2281 tcagccccga tgccgacggt cagaaaaaag tgatggaaag tatttattcc ttgaaagctg
     2341 gcaatatagc gcacgcacaa aagttgatga atgagcaggc tgaaatatta ttttcccgcg
     2401 gtgcagaaat ccttgttctt ggttgtacag aaattccggt tattctggcg caagccgtta
     2461 aagagcagcc ttcccgctat attgactcaa cggcgtcact cgttcgtgcc ggaataaaat
     2521 ggtacgaaaa tcgtgtcggt aaacattatc ttttaaccca ataatcattt aaatcgcagc
     2581 cagaaagtta ttcgctttta actgaattat atttataacg gagaacatta tggtttggct
     2641 ggaaattatc gtagtacttg gtgcaatatt ttttggtatt cgccaggggg gaatcggtat
     2701 tggtttatgt ggcgggcttg ggcttgccat tctgactctg ggatttggtc tgcctatggg
     2761 gtcgccgcca gtcgacgtga tcctgattat tatgaccgtt gttgtggcgg cctcagcctt
     2821 acaggccgca ggcggaatgg attatctggt acgcgtcgct ggtaatttta tgcggcgtaa
     2881 cccgaaatat atcaacataa tcgcgccgat tatcacctgg tctatgacca ttatggccgg
     2941 tactgggttt attgtttttt ccacgctgcc ggtgattgct gaagtggcga aagagtccgg
     3001 tatccgtcct tcccgcacgc ttgccggttc ggttgtcgct tcgcaggttg cgatttccgg
     3061 ttcgccgatc agcgccgcaa tggcggctat gctcactatt atggaggtaa acggtgtcag
     3121 ttttattcaa gtgatgtcgg tatgtcttcc cacctctttt gctgccgcga tggtcgccgc
     3181 ttttattgct tcccgacagg gatgtgagtt gcaggatgac gaggtctacc ttgagcgcct
     3241 gcaaaaagga ctggtgcaaa aatacgagaa taatagcagc atcaagccag gtgcgacgct
     3301 ctccgtcgga ttgttcctgc tggcgacggt cgctattgtt attcttgctg ccttccccca
     3361 actgcgtccg ggttttgata tcggtaaacc gatggaaaca cgcgatatca ttattatctg
     3421 tatgctctct gccgcctgcc tgatggtggt gctgtgtaag acggcgaccg atgccattat
     3481 tctcacctcc acattccgtg cggggatgag ttcgctggcg gtaattctcg gcattgtaac
     3541 cttaggcacc acttttattg atgcgcattt aactgagatt aaagatattg ctggtgatat
     3601 tttgcaggcg tatccgatgt tgttggcgct ggttcttttt ggcacttgcg cgctgctcta
     3661 ctcgcaaggt gcgaccacgc cgttaattat cccgcttgcc gtcgcgcttg acgtgcccac
     3721 ctgggcaatt ctggcgtcct atgtggccgt caccggagta tttgtactgc caacgtatcc
     3781 aacatcactg gctgccatgg aatttgatac taccggcaca actcgtgtag gcaaatatgt
     3841 gctgaatcat ccatttatgc tgcctgggtt ggggggaatt attgctggag tggcattagg
     3901 ctttgtgatt gcgccaatga ttatttagca ctatcaaaca tcagtatcga tggtttatac
     3961 acaataaaga cagatatggc ggaatatagt ctgttgtaag cgccaacaaa gaatagggga
     4021 attccgccat gtctgaacat cttcacaata tggtattaag tgttgtcgtt taatgattta
     4081 tttcttggtt atgttgtgta ttagctgcat ctatcgcttt ttgatac
//
LOCUS       DAJTNA010000265         4065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTNA010000265 DAJTNA010000000
VERSION     DAJTNA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.271"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(189..656)
                     /locus_tag="OO161_003940"
     CDS             complement(189..656)
                     /locus_tag="OO161_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464806.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
     gene            complement(1724..1915)
                     /locus_tag="OO161_003941"
     CDS             complement(1724..1915)
                     /locus_tag="OO161_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5464807.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            1983..2306
                     /locus_tag="OO161_003942"
     CDS             1983..2306
                     /locus_tag="OO161_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5464808.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            2269..3012
                     /locus_tag="OO161_003943"
                     /pseudo
     CDS             2269..3012
                     /locus_tag="OO161_003943"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2984..3544
                     /locus_tag="OO161_003944"
     CDS             2984..3544
                     /locus_tag="OO161_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5464809.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            3551..3784
                     /locus_tag="OO161_003945"
     CDS             3551..3784
                     /locus_tag="OO161_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5464810.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
BASE COUNT          973 a          930 c         1227 g          935 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt
      121 tgctattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 ttaagatatt acgttaaatt gtgagggaat ggtccccata tactgacttt atcataggga
      241 tagggttggt agtgggtcga ttgggtttcg gcattgtttc cgttaatgcc aattgcattt
      301 ttgtatttga tgcaacagaa ggcgtgagat tctcctgggc ctggaaaggc taagtgttta
      361 gaattgatgg caaaatatct tccgtccgat ttttgaagta gttgttgttt ggctgtctcc
      421 caaagggtgc tgttacaaac ttcttgaatt tgtagttttt gcagggctct tattactgcg
      481 ttgtcatgtt ccaattcttt aggggagtgt attattctct cttttaataa gctgtttacc
      541 acagggttta gttgttgtct aaagcacgca gataatgcca ttgctacaca attttgttcc
      601 cgaggaagca tgtttggcgg ggtatgccgt gtttgatgtg atatgtctat tctcatagaa
      661 ggtctctggc ttatagcatc aacaaatacg gctataaaac aaaattttca tagagtgatt
      721 caagatcttc ttgagatcct tttttgcggc gcgtaatctg ctgcctgcaa acaaaaaaac
      781 caccgctacc aacggtggtt tgtttgccgg aatctagagc taccaactct ttcttccgaa
      841 ggtaactggc ttggtggagc acagatacca aatactgtcc ttctagtgta gccgcagtta
      901 ggccaccact tcaagaactc ttaatatctc aatccacctt gtctagttac caatggctgc
      961 tgccagtggc gttaagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1021 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1081 ctacaccgaa ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1141 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1201 gccaccaggg ggaaacgcct ggtatcttta agtcctgtcg ggtttcgcca cctctgactt
     1261 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagctgc
     1321 gtggcctttt ccgggttctt gttttttgct cacatgtttc ttttggtgtt atcccctgat
     1381 tctgtggata accgcattac cgtctttgag tgagctgata ccgctcgccg cagccgaacg
     1441 accgagcgga gcgagtcagt gagcgaggaa gcggaaaagc gcctggacga gcattttctc
     1501 cttacgcatc tgtgcggcat ttcacacccg gcatggtgca cttttcatac aatcctcact
     1561 gatgccaaat gattaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     1621 cgccccgaca cccgctaaaa cctgctgacg cgccctgacg ggcttgtctg ctcccggcat
     1681 ccgctcacag gccagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     1741 catccccgaa gcgtgcgagg cagctgcggt aaagctcgtc agcgtggtcg tgaagcgctt
     1801 cgcagatgtc agcctggtcg tcggcgtcca gttcattcag tttttccagc agggtcagcg
     1861 tctggcttct gatgaagcgg gccatgttaa gggcggtttt ttcctgttta gtcacagttc
     1921 tgtttctcca tgtgtgggga gtctgtcgga ggtttttaat gataccggtg aaacgcgaaa
     1981 ggatgctcac gatacgggtc acggatgagg aacatgcgcg gctgctggca cgttgtgagg
     2041 gaaaacaact tgcgtcatgg atgcgtaagg tctgcctggg ggcaccgcca tcgaaaacgt
     2101 caggtcttcc gacactggca ccgccgctgt tgcgccagtt cgcttcggtc ggcaataacc
     2161 tgaatcagat agcccggaag ataaacagcg gacagtggtc ggggcatgac cgggttcatg
     2221 tggtggctgc actgatggcc atcggacgtg aactgagtga gctgagggat gaggtccgta
     2281 aacagggaga gcgggatgat agttaagttt catgccaggg gaaaaggcgg tggcagtggt
     2341 ccggttgatt acctgctggg ccgggagcgt aaccgggaag gggcgagagt tctgcggggt
     2401 gctcccgaag aggtgcggga actcatcgat gctacgccgt ttgcgaaaaa atacacctcg
     2461 ggggttctgt cttttgcaga gcagaccctg ccaccgggag agcgtgaaag ggtgatggag
     2521 agctttgagc gggtactgat gcccggtctt gaaaaaaatc agtacagcat cctgtgggtg
     2581 gagcaccagg acaaggggcg tcttgagctg aattttgtca tcccgaatat ggagctggcg
     2641 agcggaaaac ggctccagcc ctattacgac cgagcggacc gtccgcgaat caatgcctgg
     2701 cagacactgg tgaatcacca ttacgggctg cacgacccga acgcaccgga gaacaggcgg
     2761 acacttgtca ccccgaataa tctgccgaaa gcgaaacagg aggccgcaga agcgattacg
     2821 aggggactgg aggctcttta ccatgccgga gcgctaaaaa cgcgtcagga tgtcactgag
     2881 gcgcttacag cggcaggttt tgaggttgtg aggacaacca ggagcagtat cagtatcgcc
     2941 gacccggagg gcgggcgtaa tctcaggctg aaaggggcgc tgtatgagca atcttttaca
     3001 aatggggaca gacttcgaga agaaactgaa agagcgtgcc gtctctacag agaacatact
     3061 gaacagcgag ttcaggaagc tcgaagcgtc tgtagacagg gaactgaact taaacgggca
     3121 gaaaatcaga gacgctatca gcgcacacac agcagcggtg aaagaacaac tggagacgct
     3181 gaacagtgcc gtgaacgcgc agttcagcgc cacagagaac gaactggccc ggcggcagga
     3241 agagctgctc tggaagctgg tcaaagggcg gatactgtac ccgtcgctga cggcgctttg
     3301 cgtcacgggc gggatttttc tggcgtcatg ggggctgatt cagtggcagg aaagccggat
     3361 tgcggcgaac atcctggcta tcagggacca ggaagagacg ctggcgaaac tccgggaaaa
     3421 aacatggggt gtgacgtatc acgagggcag gaacgggaaa tttctggtat tgccgtccgg
     3481 ggtgaagggg gaaaacaact ggacggtgga gaagaagaac gcagtaagac tggtcaggga
     3541 gtaagtgagt atgacagagc tggaaatgca cttactgaac gccttagagc aattgcagca
     3601 ggactatacg cagcggctga aagactggga gagcgccttc acggatttgc agcatatgtt
     3661 cgagctgaca cgacaggaga acgggcaact gagggaacag tgcgacgact tgagtcagca
     3721 ggtgcagaga ttagtcgagc aggtggacca cttgagtcgg ttatttatca cgaacagcag
     3781 ataagggatg aacaggaaag gcaggcacgc ctggaacggg agcgtgcgcg gtcctggcat
     3841 gaagagcagg aacgggaagc acgggtatgg cgtggcccgt caatagggat atgacggtga
     3901 gttgttccgg gaggttgcca gatggtaccg gaacgcgtta aaaacgcatc cgggcgcgat
     3961 tctggcgtgt cgtttttgtt gctgtgtttt gcctctttgc cagatcgcgc tcattcctca
     4021 gcctcatccc gcgatgtctg cgtatgtcat caactgaaaa gatgg
//
LOCUS       DAJTNA010000266         4053 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTNA010000266 DAJTNA010000000
VERSION     DAJTNA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.272"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..301
                     /locus_tag="OO161_003946"
     CDS             <1..301
                     /locus_tag="OO161_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5464811.1"
                     /translation="GQNLYLDNLEATGLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYC
                     GDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHQAWHASAFTGIYNDLVAASTFV"
     gene            238..840
                     /locus_tag="OO161_003947"
     CDS             238..840
                     /locus_tag="OO161_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5464812.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLNE
                     GWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVASQEISTADEGDGGQVVVIGR"
     gene            913..1251
                     /locus_tag="OO161_003948"
     CDS             913..1251
                     /locus_tag="OO161_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464813.1"
                     /translation="MNKTILFCTIIALTGCKSLDYVKSGKPVMEGNSLKNIDELSGCI
                     SRQWAGNGTPITSLPIENGVSLLVPQAMGGYDIVLDIKKAGNGSSFTLYERVPALTPK
                     IFADSVNACK"
     gene            1318..>4053
                     /locus_tag="OO161_003949"
     CDS             1318..>4053
                     /locus_tag="OO161_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5464814.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRYMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFRQ
                     LALGRYMLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRKVETTARYLGTGLYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDKGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLKDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGI"
BASE COUNT          957 a          977 c         1240 g          879 t
ORIGIN      
        1 cggtcagaat ctctatctgg ataatctgga ggccacaggg ctgtatcagg tgccgttgtc
       61 agcggcgcag ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc
      121 cgccatttac tgtggtgacg gcgagctgct gcaccatatt cctgaacaac tgagcaaacg
      181 agagaggtac accgacaaat ggcagcgacg cacacactcc ctctggcgtc accaggcatg
      241 gcacgcatct gcctttacgg ggatttacaa cgatttggtc gccgcatcga ccttcgtgtg
      301 aaaacggggg ccgaagccat ccgggcgctg gccacacagc tcccggcgtt tcgccagaaa
      361 ctgaatgagg gctggtatca ggtgcgcatt gccgggcgtg atgcaggcga aaacgaatta
      421 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt accgcgtctg
      481 gtgggagcta aaagtggcgg tgtgtttcag gcggtgctgg gggcagctgt tatggcggtt
      541 gctatatgga tgccgggggt aggaattatg gcgagtaatc tgctgttttc tctcggtgcc
      601 agtatgacgc ttggcggtgt tgcacagatg ctggcaccga aagccagaac tccccgtaca
      661 cagacaacgg ataacggcaa acagaacacc tgtttctcct cactggataa catggttgcc
      721 cagggcaatg tcctgcctgt tctgtacggt gaaatgcgcg tggggtcacg tgtggcatct
      781 caggagatca gcacggcaga tgaaggggat ggtggtcagg ttgtggtaat tgggcggtaa
      841 tattatttac tcatgttcta actgatttaa tatttatatc gaacactgat aattattcta
      901 ttggttagct atatgaacaa aacgatttta ttctgcacga ttattgcctt aacaggatgt
      961 aaatctttgg attacgtaaa atccggaaaa cctgtaatgg aaggtaattc attaaaaaat
     1021 attgatgaat tgtcaggctg catatccaga caatgggctg gtaatggaac acctataaca
     1081 tcccttccta ttgagaatgg ggtaagcctt ttagttccac aggctatggg gggatatgat
     1141 attgtgcttg atatcaaaaa agcaggaaat ggcagtagtt ttactcttta tgaacgtgta
     1201 ccagcattaa cgccaaaaat ttttgctgat agtgttaatg catgtaaata atagttaatc
     1261 ctgccgtaac tcatgagccg ccttttgggc ggctttgttg tttatggagt gtgaggaatg
     1321 ggtaaaggaa gcagtaaggg gcataccccg cgcgaagcga aggacaacct gaaatccacg
     1381 caattactga gtgtgatcga tgccatcagc gaagggccga ttgaaggtcc ggtggatgga
     1441 ttaaaaagcg tgctgctgaa cagtacaccg gtgctggaca gtgaggggaa taccaacatc
     1501 tccggtgtta cggtggtgtt ccgggcaggt gagcaggagc agacaccgcc ggagggattt
     1561 gaatcctccg gttccgagac ggtgctgggt acggaagtga aatacgacac gccgatcacc
     1621 cggaccatca cgtctgcaaa catcgaccgt ctgcgcttta ccttcggtgt gcaggcactg
     1681 gtggaaacca cctcaaaggg tgacaggaat ccgtcggaag tccgcctgct ggttcagata
     1741 caacgtaacg gtggctgggt gacggaaaaa gacatcacca ttaagggcaa aaccacctcg
     1801 cagtatctgg cctcggtggt ggtgggtaac ctgccgccgc gcccgtttaa tatccggatg
     1861 cgcaggatga cgccggacag caccacagac cagctgcaga acaaaacgct ctggtcgtca
     1921 tacactgaaa tcatcgatgt gaaacagtgc tacccgaaca cggcactggt cggcgtgcag
     1981 gtggactcgg aacagttcgg cagccagcag gtgagccgta attatcatct tcgcgggcgc
     2041 attctgcagg tgccatcgaa ctataacccg cagacgcggc aatacagcgg tatctgggac
     2101 gggacgttta aaccggcata cagcaacaac atggcatggt gtctgtggga tatgctgacc
     2161 cacccgcgct acggcatggg gaaacgtctt ggtgcggcgg atgtggacaa atgggcgctg
     2221 tatgtcatcg gccagtattg cgatcagtca gtgccggatg gctttggtgg cacggagccg
     2281 cgcatcacct gtaacgctta cctgaccaca cagcgtaagg cgtgggatgt gctcagtgat
     2341 ttctgttcgg cgatgcgcta tatgccggta tggaacgggc agacgctgac gttcgtgcag
     2401 gaccgaccgt cggataaggt gtggacctat aaccgcagta atgtggtgat gccggatgat
     2461 ggcgcgccgt tccgctacag cttcagcgcc ctgaaggacc gccataatgc cgttgaggtg
     2521 aactggattg acccggataa cggctgggag acggcgacag agcttgttga agatacgcag
     2581 gccattgccc gttacggtcg taacgtcacg aagatggatg cctttggctg taccagccgg
     2641 gggcaggcac accgcgccgg gctgtggctg attaaaacgg agctgctgga aacgcagacc
     2701 gtggacttca gcgtgggtgc agaagggctt cgccatgtac cgggcgatgt cattgaaatc
     2761 tgtgatgatg actatgcggg tatcagcatc ggcgggcgcg tgctggcggt gaacagccag
     2821 acgcggacac tgacgctcga ccgtgaaatc acgctgccat cctccggcac cacgctgata
     2881 agcctggttg acggacaggg gaatccggtc agcgtggagg tccagtccgt caccgacggc
     2941 gtgaaggtga aagtgagccg tgttcctgac ggcgttgccg aatacagcgt gtgggggctg
     3001 aagctgccga cgctgcgcca gcgcctgttc cgctgcgtga gtatccgtga gaacgatgac
     3061 ggcacgtatg ccatcaccgc cgtgcagcat gtaccggaaa aagaagccat cgtggataac
     3121 ggggcgcact ttgacggcga ccagagcggc acggtgaatg gtgtcacgcc gccagcggtg
     3181 cagcacctga ctgccgaagt caccgcagac agcggggagt atcaggtact ggcccgctgg
     3241 gacacgccga aggtggtgaa gggcgtgagc ttcctgcttc gcctgaccgt gacagcggac
     3301 gatggcagtg agcggctggt cagcacggcc cggacgacgg aaaccacata ccgcttcagg
     3361 cagctggcgc tggggcgtta catgttgacg gtccgggcgg taaatgcgtg gggacagcag
     3421 ggcgatccgg cgtcggtatc gttccggatt gccgcaccgg cagcgccgtc gcggattgag
     3481 ctgacgccgg gctattttca gataaccgcc acgccgcatc ttgccgttta tgacccgacg
     3541 gtacagtttg agttctggtt ttcggaaaag cggattaccg atatcaggaa ggttgaaacc
     3601 acagcgcgct atcttggcac ggggctgtac tggatagccg ccagtatcaa tatcaaaccg
     3661 ggccatgatt attattttta cgttcgcagt gtgaacaccg ttggcaaatc ggcattcgtg
     3721 gaggctgttg gtcagccgag tgatgatgca tccggttatc tggatttttt caaaggcgag
     3781 atagggaaaa cccatctggc tcaggagctg tggacgcaga ttgataaagg tcagcttgcg
     3841 cctgacctgg ctgaaatcag gacgtccatt acggatgtca gcaatgaaat cacgcagact
     3901 gtcaataaga aactgaaaga ccagagtgcg gcaatccagc agatacagaa ggttcaggtt
     3961 gatacaaata ataatctgaa cagcatgtgg gctgtgaagc tgcaacagat gaaggacgga
     4021 cgcctttata ttgcgggtat cggtgccggt att
//
LOCUS       DAJTNA010000267         4036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTNA010000267 DAJTNA010000000
VERSION     DAJTNA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.273"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..603)
                     /gene="mcbR"
                     /locus_tag="OO161_003950"
     CDS             complement(<1..603)
                     /gene="mcbR"
                     /locus_tag="OO161_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5464815.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCR"
     gene            complement(801..1838)
                     /gene="curA"
                     /locus_tag="OO161_003951"
     CDS             complement(801..1838)
                     /gene="curA"
                     /locus_tag="OO161_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5464816.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHHAGDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPRTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            2019..2537
                     /gene="mddA"
                     /locus_tag="OO161_003952"
     CDS             2019..2537
                     /gene="mddA"
                     /locus_tag="OO161_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5464817.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            2534..2983
                     /locus_tag="OO161_003953"
     CDS             2534..2983
                     /locus_tag="OO161_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5464818.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFCELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARHSF"
     gene            complement(2984..3217)
                     /locus_tag="OO161_003954"
     CDS             complement(2984..3217)
                     /locus_tag="OO161_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5464819.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(3303..3476)
                     /gene="ortT"
                     /locus_tag="OO161_003955"
     CDS             complement(3303..3476)
                     /gene="ortT"
                     /locus_tag="OO161_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5464820.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            3671..3766
                     /gene="yncL"
                     /locus_tag="OO161_003956"
     CDS             3671..3766
                     /gene="yncL"
                     /locus_tag="OO161_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5464821.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          987 a         1018 c          914 g         1117 t
ORIGIN      
        1 tctgcatccc tctttgtctt ttgcttttaa cgcagcgagt aacagatgat agttttctat
       61 atgctcccgt aattccgctg gattaatcgc ttcatagaga taatgtaaac caggccccat
      121 cctgacccac agttgctcaa tcatctcaca caggatgggc atatttgagc gatgataaat
      181 cgctaagcga aatagcctgt ttacattaat gatttgttcc atgtcgccct tttcttgcgc
      241 ctgctgtaat ttctcgagca attcctggag ttccgccagg tcttgcgggg tgaggttttc
      301 aacagccaga gcaactgcca ttaattccag ttcgtagcgg atccgattga tttcgtctaa
      361 ttgacgtttc cccacttccg gaactgtaaa tgcttgtgca ggtgcgacag aaagcgcatt
      421 caccgaaacc agacgtaata atgcttcacg cacaggtgta atactcatac ctaattgctc
      481 cgccagattt ttagtaatca ggcgtgcgcc aggttttaat gcgccaatac taagctgatg
      541 tttcaggtcg ttctcaacct gcagagtcaa actgacatgt ttcatttttc ccattccagg
      601 cattttaatt ccttcgcgtg ttttctgtcc aaccctgcct gcacggcaga aggagttcac
      661 ctggtgtatt aaagtgaata atcataaacc acccaccttt gttgttatta gcagcagaac
      721 aatgacgtgg ttagttaatt aaagctgaaa ataagctttt tgcttggatt tttatggcgg
      781 cttacgccgc cgttgaaact ttaatcatca cccgcgacgc ggatcaccac tttgccgaag
      841 tttttaccct tcagcaggcc gataaacgtc cgtggcgcat tctctaaacc atcagtaata
      901 tcttcgtggt agtggatttt atcctctttc acccattgcc ccatctcctt ctgaaactca
      961 tggatgcggt gaccataatc ctgagcgata ataaaccctt gcaagcgaat acgttttttc
     1021 agcaccgttg ccatcaacag gggtaaacga tccggaccgg gtggtagctc tgtagcgtta
     1081 tagctgctca ctaagccaca aacgggaatg cgcgcagatg tattaagtaa tggtagcact
     1141 gcatcaaata ccttaccgcc cacgttttca taatagatat caataccttt tgggcacgct
     1201 ttcaccagtt gttcggcaaa atcacccgcg tggtgatcaa gacaaacatc gaagcccagc
     1261 acctcgatag catggcgaca tttttccgcg ccaccggcac cccccaccac cctgcaacct
     1321 ttaagtttgc cgatttgccc caccgttgct cccacaggac ctgtcgccgc agctaccacc
     1381 aacgtttcgc cctctttagg ctgaccgata tccagcagcc ccatataagc ggtaaagcct
     1441 ggcatcccca gcacacccag cgaccacgat ggattttgcg gatgatcgcc aagtttcacc
     1501 agatcatcac cactggatat gtcatagtcc tgccatccac tgtagcccag cacccagtcg
     1561 ccaggctgat aatcaggatg attcgactcc acaacatggc tcaccgtgcc gccgaccatc
     1621 acgccgccaa tatcaacagg cggtgaataa gatggctcat cgctcatacg tccacgcata
     1681 tacgggtcca gggacaaata aactgtgcgc agtaacacct gtccttcacc cggtgtggcg
     1741 acatcatctt cttcaagacg gaaattctcc ggaaccggtg cgccatgtgg acgcgaggcc
     1801 agaacccaac gtcgattacg ctgcttttgt tgccccataa tgctctcctg tgcttttggc
     1861 atgaatccat caatagtagc aggctcacgg gattggtttt gtttgcagcc gtcacatccc
     1921 ctctgcaaca cgcaataatt tcatcgagga atccgttgct tcgggctgtt ttcgatagta
     1981 ttgataacat catcatcatt attttatcga ggccagacat gtccatccgt tttgcccgca
     2041 aagccgattg tgctgccatt gcggaaattt ataaccacgc cgtgttgtat acggcggcta
     2101 tctggaatga ccaaacggtt gatgcagata accgcattgc ctggtttgaa gcgcggacca
     2161 tagcaggtta tccggtgctg gtaagtgaag aagatggcgt agtgacggga tatgcctcgt
     2221 ttggcgactg gcgtagtttc gatggttttc gccataccgt ggaacattcg gtttatgtcc
     2281 atcccgatca tcagggcaaa ggtctggggc gtaaattgtt aagccgattg attgatgaag
     2341 cgcgggatag cgggaagcat gtcatggtcg ccgggatcga atcgcaaaat caggcctcgc
     2401 tgcatctgca ccattcgctg ggatttgtcg tcaccgcaca aatgccgcag gtgggcacta
     2461 aatttgggcg ctggctggat ctgacattta tgcagttgca actcgacgaa cgcactgaac
     2521 cggacgcgat tggatgaatc agtcgctcac ccttgctttt ttgattgccg ccggaattgg
     2581 tctggtagtc cagaacacgc tgatggtgcg catcacccag acctcctcta ccattctcat
     2641 cgccatgttg ctgaactcac tggtggggat tgtgctcttt gtttctattt tatggtttaa
     2701 acaaggcatg gcggggtttt gcgaactggt gtccagcgtg cgctggtgga cacttatacc
     2761 cggcttgctg ggatcgtttt ttgtcttcgc cagtatcagc ggatatcaga atgtgggagc
     2821 ggcgacaact attgcagtac tggtcgccag ccagctaatt ggcggtctga tgctggatat
     2881 cttccgcagt catggcgtac cactacgggc gttgttcggc ccaatttgtg gcgcaatttt
     2941 acttgtggtt ggcgcctggt tggtagccag acactcattt taatcagagg atggtgccgc
     3001 ctttggttaa ctgttcgtgg cgcgcttcca tatcttcttt atgcttgcga ccgtgatgag
     3061 caatagcggt gcgcaaacgc tgctgctggg tgtagcgatc ttcccgactt aactccgcgt
     3121 catcgctcag ggcgattaat aattcgttca tatgggcaat gacgctctct tcaatcgctg
     3181 catccacgcg cgcgatgact tcatccagat gcgacatact ttctccttaa ccacttgtga
     3241 aatttatcga ttcatccaaa tgatataagt ctgcgtggtg ccgtcaagcc agtgagtaag
     3301 atttaaaaca gtgaaaacaa cagcgccacc gggaaactca tcggccatgt tgcccccacc
     3361 agaaaggcgc ttaagaaacg gatgcgtttc ttatcgtgag aaagaaacca ggtgataaga
     3421 aatgcgattg ctgccataac cgcataaaaa acaagcatat gttgatagag agacatgtac
     3481 gggcgtccag ccagatgaat aaaacgcgcg caaatatgcg tcattttttg acgtgcatca
     3541 agttttttca ccagtttgag aaaaaatcat caaacagcaa cttgccctta tccatcttca
     3601 gaatgagtat tattgcggat tttcttaacc cgtactatac acataggggc aatgataaaa
     3661 ggtggcaaaa atgaatgttt ccagtagaac tgtagtactg ataaatgtct ttgctgctgt
     3721 tggtttgttt actcttatct ctatgagatt tggctggttt atttgatgta gtgattttgc
     3781 acgcagcacg tactgtcaga gaaatcaact ctctgacagt acgcaagatc ttctcatact
     3841 ctccaggttt acaacattat ccttaatatt tacttctttt tgctggcatc tacattgaaa
     3901 tgagtagaaa acatccccct actgtcgtct atacaaacag cttactgatt caaacggaat
     3961 cttgttttca catttttcta tcatgaatac tttatacgcg cgggagtcta taaatgctga
     4021 ggtagcctga gtttaa
//
LOCUS       DAJTNA010000268         4032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTNA010000268 DAJTNA010000000
VERSION     DAJTNA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.274"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            229..1509
                     /gene="wcaK"
                     /locus_tag="OO161_003957"
     CDS             229..1509
                     /gene="wcaK"
                     /locus_tag="OO161_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5464822.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNQHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASDAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGIQMVQSVLERIGEVK"
     gene            1506..2726
                     /gene="wcaL"
                     /locus_tag="OO161_003958"
     CDS             1506..2726
                     /gene="wcaL"
                     /locus_tag="OO161_003958"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5464823.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     TLAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGGTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            2737..>4032
                     /gene="wcaM"
                     /locus_tag="OO161_003959"
     CDS             2737..>4032
                     /gene="wcaM"
                     /locus_tag="OO161_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5464824.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVLGSDCTINGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFAIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIRV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAV"
BASE COUNT         1010 a         1062 c         1049 g          911 t
ORIGIN      
        1 gccttatcca tcctacggtt ctgtgctcgg gacctgtagg cctgataaga cgcgtcagcg
       61 tcgcatcagg cactgaatgc cggatgcggc gtgaacgcct tatccggcct acggttctgt
      121 gctcgggtct tgtaggcctg ataagacgcg ccagcgtcgt atcaggcaaa aaacggcaac
      181 gaacacaaca aacacagcct cacctggcac caaatttatg aggaaaaaat gaaattactt
      241 attctgggca accacacttg cggcaatcgt ggcgacagcg ccatcttgcg cggcttactt
      301 gatgccatca acatcctcaa tcaacatacc gaagtggacg tgatgagccg ctatccggtc
      361 agttcttcct ggctgctcaa ccgcccggta atgggcgatc cgctgttcct gcaaatgaaa
      421 caacacaaca gcgcggcggg cgttgtcggg cgcgttaaaa aagtccttcg tcgccgctat
      481 caacaccagg tattgctctc acgcgtcact gacactggca agctgcgtaa tatcgccatc
      541 gcccagggat tcaccgactt cgtgcgcctg ctgtcaggtt acgacgccat tattcaggtc
      601 ggcggatcgt tttttgtcga tctctacggc gtgccgcagt ttgaacatgc gctttgcacg
      661 tttatggcga aaaagccgct gtttatgatt ggtcacagcg tcggtccctt ccaggatgag
      721 caatttaacc aactggcgaa ctacgttttt ggtcactgtg acgcgctgat cctgcgcgaa
      781 tcggtcagcc ttgatttgat gaaacgtagc aatattacta ccgcaaaagt ggaacatggc
      841 gttgataccg cgtggctggt cgatcaccac accgaagatt tcaccgccag cgatgccgtc
      901 caacactggc tggacgttgc cgcacaacag aaaacggtgg caattacctt gcgcgaactg
      961 gcaccgttcg acaaacgtct cggcaccact caacaagcgt atgaaaaagc ctttgccggg
     1021 gtggtcaatc gcattctcga cgaaggctat caggttattg cgctctccac ctgtacgggc
     1081 atcgacagct ataacaaaga cgaccgcatg gtggcgctca atcttcgcca gcacatcagc
     1141 gatcctgccc gttaccacgt agtgatggat gaacttaacg atctggaaat gggcaaaatt
     1201 ctgggggcct gtgaactcac cgtcggtacg cgcctgcact ccgccattat ctcaatgaac
     1261 tttgccactc cggcgattgc catcaactac gaacataaat ccgccgggat tatgcagcag
     1321 ctgggactgc cagagatggc gattgatatc cgtcatttac tggacggcag cctgcaagcg
     1381 atggttgcgg ataccttagg ccagcttccg gcgctgaacg cacgacttag cgaagccgtc
     1441 agtcgtgagc gtcagacagg aatacagatg gtgcaatctg tgcttgaacg catcggggag
     1501 gtgaaatgaa ggtcggcttc tttttactga aatttccgct gtcgtcggaa accttcgtcc
     1561 tcaatcaaat taccgcgttt attgatatgg gatttgaggt ggagattgtc gcgctgcaaa
     1621 aaggcgacac acaaaacacc ctcgcggcat ggacgaaata caaccttgcc gccagaaccc
     1681 gctggttaca ggacgaacct acgggcaaag tggcgaagct gcgccaccgc gccagccaga
     1741 cgttacgcgg cattcatcgt aaaaatacct ggcatgcgct caacctcaaa cgctatggtg
     1801 ccgagtcgcg gaacctgatt ttgtctgcca tttgcggcca ggtcgcaaca ccgtttcgcg
     1861 ccgatgtgtt catcgctcat tttggcccgg cggggggaac cgcagcaaaa ctacgcgaac
     1921 tgggtgtcat tcgcggcaaa attgccacta ttttccacgg cattgatatt tccagtcggg
     1981 aagtgctcaa ccattacact cccgaatatc agcaactgtt tcgccgtggc gatctgatgt
     2041 taccgataag cgatttgtgg gccggaaggc tgcaaaaaat gggctgcccg agggaaaaaa
     2101 ttgccgtatc gcgtatgggt gtggacatga cgcgctttag cccgcgtccg gtgaaagcgc
     2161 ctgcaacgcc gctggaaatc atttccgtcg cacgcttaac cgagaaaaaa ggcctgcatg
     2221 tggcgatcga agcctgccgt cagttgaaag agcagggcat ggcattccgc tatcgcattc
     2281 tcggcattgg cccgtgggaa cgacgcctgc gcacgctcat cgaacaatat caactggaag
     2341 atgtggtaga gatgcctggc tttaaaccga gccatgaagt gaaagcgatg ctcgacgacg
     2401 cggatgtctt cctgttgcca tcggttacag gtgcggatgg cgatatggaa ggcattccgg
     2461 tggcgctgat ggaggcgatg gcggtcggca ttccggtggt ttctactctg catagcggaa
     2521 taccggaact ggtggaggct gacaaatccg gctggctggt gcctgagaac gatgctcgcg
     2581 cactggcgca acgactggcg gcgtttagcc aactggacac cgacgaattg gctccggtcg
     2641 tcaaacgcgc gcgcgaaaaa gttgaacacg attttaacca gcaggtgatc aatcgagaac
     2701 tcgccagctt gctgcaggct ttatagagga gaacgtatgc catttaaaaa actctcccga
     2761 cgcaccttcc tgacggcaag ctcggcgctt gccttcctcc ataccccttt cgctcgcgca
     2821 cttcccgccc gacaaagcgt taacattaac gactacaacc ctcacgactg gatcgcctca
     2881 tttaaacaag ccttcagcga agggcaaacg gtcgtcgtgc ctgctggatt cgtttgtgac
     2941 aatatcaaca ccggcatctt cattcctcct ggcaaaacgt tacacatcct tggaagcctg
     3001 cgcggcaacg gcagagggcg atttgtttta caggacggca gccaggtgac aggggaggag
     3061 ggaggcagta tgcataacat caccctggat gtgctaggct ctgactgcac catcaacggg
     3121 ctggcgatga gcggctttgg tccggtaacg cagatttata tcggcggcaa aaacaaacgg
     3181 gtcatgcgca acctgaccat cgataacctc actgtcagcc acgctaatta cgccatctta
     3241 cgccagggat ttcataacca gattatcggt gccaacatca ccaattgtaa gttcagcgac
     3301 ttacagggcg acgccattga atggaacgtg gcaattaacg acagtgatat tttgatctcc
     3361 gaccatgtca tcgagcgcat caactgtact aacggaaaaa tcaactgggg cattggcata
     3421 ggccttgcgg ggagtactta tgataataac tacccggaag accaagcagt gaaaaacttt
     3481 gtcgtggcaa atatcacggg atcggattgt cggcagttga tccatgttga aaatggcaaa
     3541 cattttgtta tccgtaatat caaagcccgc aatatcacgc cggatttcag taagaaagca
     3601 ggcattgata acgcgacagt tgctatttac ggttgtgaca atttcgcgat tgataatatt
     3661 gaaatgatta atagcgccgg gatgttaatc ggctatgggg taattaaagg caaatatctc
     3721 tcgataccgc aaaatttccg agtgaataat attcaactgg ataacaccca tcttgcttat
     3781 aaattgcgcg gcatccaaat ctccgccggg aatgctgtct cctttgtggc actgactaac
     3841 attgagatga agcgtgcctc gcttgagcta cacaataaac cgcaacatct ttttatgcga
     3901 aatatcagag tgatgcagga atcctcagtt ggaccagcat tgagcatgaa ctttgacatg
     3961 cgcaaagacg ttcgcggcgt gtttatggcg aaaaaagaaa cactgctgtc tcttgcaaat
     4021 gttcatgcgg tg
//
LOCUS       DAJTNA010000269         4031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTNA010000269 DAJTNA010000000
VERSION     DAJTNA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.275"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            2..235
                     /locus_tag="OO161_003960"
     CDS             2..235
                     /locus_tag="OO161_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464825.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
     gene            527..682
                     /gene="hokD"
                     /locus_tag="OO161_003961"
     CDS             527..682
                     /gene="hokD"
                     /locus_tag="OO161_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5464826.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            899..1150
                     /locus_tag="OO161_003962"
     CDS             899..1150
                     /locus_tag="OO161_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464827.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQVLKEIGIKAGFSAFSEE"
     gene            1217..1495
                     /locus_tag="OO161_003963"
     CDS             1217..1495
                     /locus_tag="OO161_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464828.1"
                     /translation="MARNAKYYKHDNSTVLAHTHERYSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRLSRTYQQRIWR"
     gene            1497..2543
                     /locus_tag="OO161_003964"
     CDS             1497..2543
                     /locus_tag="OO161_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5464829.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTSAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEETVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            2556..2930
                     /locus_tag="OO161_003965"
     CDS             2556..2930
                     /locus_tag="OO161_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5464830.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLILSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRMGVKIYPIMHEEQVKK"
     gene            2927..3748
                     /locus_tag="OO161_003966"
     CDS             2927..3748
                     /locus_tag="OO161_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5464831.1"
                     /translation="MKLEDLPKYYSPKSPGLTDVSASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQYPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
BASE COUNT         1036 a          910 c         1136 g          949 t
ORIGIN      
        1 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
       61 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      121 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      181 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      241 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      301 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      361 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      421 aacattacgc gtccggcaag acgcttaaca ttaatctgag gccatatcta tgctctacac
      481 acgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
      541 ggcgatgcta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
      601 gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac
      661 agcttacgaa cctgaggagt aagagacccg gcgagggaga aatccctcgc cacctctgat
      721 gtggcaggca ttctcaacgc acccgcactt aacccgcttc ggcgggtttt tgtttttatt
      781 ttcaacgcat ttgaagttct ggacggtgcc ggaatagaat caaaaatact taagtagcgc
      841 gcagggataa gagggatggt cccttaaagg ggagagctaa ttatccggaa ggattctgat
      901 gatgaacatc gaagaactgc gtaaaatttt ttgtgaagat ggcctctatg ctgtgtgcgt
      961 tgaaaatgga aatattgtta gtcattaccg cattgtgtgt ttgcaaaaaa atggggctgc
     1021 gttaattaat tttgtggatg cccgagtgac ggacggattt atcttgcgcg acggtgagtt
     1081 tgtcacttca ttacaggtat tgaaagagat cggaataaaa gctggctttt ctgctttttc
     1141 agaagaataa actcatctac aatcttgcgc ggggctgaac tcccgctgag taacaccgtg
     1201 ccaccggaga aaaccgatgg cacgcaacgc aaaatattac aaacatgata attcgaccgt
     1261 tcttgcccac acgcacgagc ggtattctca cgcatttaag tcagactggt accagcatcc
     1321 cccatgcact gaagaacagg ccgaatggct cattcagtgt taccgcaggc gcggatgcga
     1381 ggttaaaaaa gcccttagcc tcgactaccg tcactggata atctccgtca ggcttcctta
     1441 ctccgaacgc ccaccgcgtc tgtcccgcac ataccagcaa cgcatctgga gataacgtgc
     1501 gggtattact tcgacctgtt ctggtaccgg aactcgggct ggtgatcgtt aagccgggcc
     1561 gtgaatccat gccggtattc cacaataccc gggtactggt ggagccggaa ccgaaaagca
     1621 tgcgtaatct gccgtccggg gtcgttcctg ccgttcgcca gccgctggcg gaggataaat
     1681 cattactgcc atttttcagc gacgaacgag tgattcgtgc tgctggtggc gctggcgcat
     1741 tgtctgactg gttactgcgc catgttaaat cctgccagtg gccacacggc gattatcacc
     1801 acagtgaaac cgtcattcac cgttatggta ccagcgcaat ggtgttgtgc tggcactgcg
     1861 acaaccagct gcgcgaccag acctccgaat cactcgggca acttgctcac caaaacctgt
     1921 ctgcatggat gattgacgtc atacgccatg caatgaatgg ctcgcaggaa cgggaattat
     1981 cgctggctga attatcctgg tgggcggtcc gcaatcaggt ggcggacgcg ctaccggaag
     2041 cggtattacg tcgttcgctg gggttgcgtg cggaaaaaat ccgctcaatg taccgtgaaa
     2101 gcgacatcgt accgggagag cagaccgcca ccagcatact gaagcagcgc acaaaaaatc
     2161 ttgcgccgct gcctcacgcc caccagcaaa acccgccaca ggaagagacg gtggtcagca
     2221 ttgccgttga tcctgagtct ccggaatctt tcatgaaacg acctaaacgt cgccgctggg
     2281 ttaacgagaa atacacacgc tgggtgaaga cacagccgtg tgcgtgttgt ggtaagccag
     2341 ccgacgatcc ccatcacctg attggtcatg gtcagggcgg aatggggaca aaatctcacg
     2401 atattttcac gctaccgctg tgtcgggagc atcacaacga gcttcatgcg gatcctctgg
     2461 cgttcgaaga aaagcatggt tctcaggttg atttaatttt tcgttttctt gatcacgcct
     2521 ttgcaactgg cgtgcttggg taaaagaggt gactgatgct catagatttg gttttacctt
     2581 acccgccgac ggtgaacact tactggcgac gccgtggcag cacatatttt atctcggagg
     2641 agggaaagcg ttatcgccgg gctgtggcgc ttattgttcg ccagcagcgg ctgaaattaa
     2701 tcctgtccgg aaggctggcg ataaaggtga ttgcagagcc accggataag cgtcgtcgcg
     2761 acctggacaa tatcctgaaa gcaccgctgg atgcgctgac gcatgcggga gtgttaatgg
     2821 acgatgagca gtttgatgaa atcaatatcg ttcgtggtca gccagtatct ggtggacgta
     2881 tgggggtgaa gatttacccc ataatgcatg aagagcaggt caaaaaatga aactggaaga
     2941 tttaccgaaa tactactccc caaaatcccc tggcctgacc gatgtatcgg cctcaacgtc
     3001 aaaagatgcg ctgagtatca ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc
     3061 tgagatgggg ttttctgcgt tcctggggaa aatgggcatc agtatgaatg acagggcgcg
     3121 ggcaacagaa ttactggcag attatgcact cagtcggtgc gatcgtgtgg cggcgttgag
     3181 aaaacttccg gcagaaataa aaccggtagt gatgcgcatt atggcttcgt acgcttttga
     3241 ggattatgcc cgcagcgcag cgagtaaaaa gcagtaccct tgttgctatg gggaaaaatt
     3301 tattgaaagc gtagttttta caaacaaggt ccagtatccg gatggtaagc cgccggtatg
     3361 ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa aagtcaggga
     3421 ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca ccgcctgtaa
     3481 ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac gtggtatgcc
     3541 tgttatcaga gactgccagc gttgtggtgg tcgtggctgt gaaagactac catcaacgga
     3601 ggcatttaat gccatatgca aagtgacgag tgctatcacg cttgatacgt ggaaaaaatc
     3661 agtgaaacgc ttttacgata cgttggtggt tcggtttgac attgaagagg catgggcgga
     3721 gcggcagtta aagagggtaa cgcgatagtg ttgttgattt ttcccgaatc tgtggtaaat
     3781 ttgctctaac gatgggcgtt ttatgcctga cgttagaaga ttttttacac cccgccgcct
     3841 ggcgggtttt ttatgactga aatcgcgtca gtacagtaaa cgcgctggtg gcggtgaata
     3901 cctgtctttc agcttgctgg ctttttcgac aagagttatt ggtgtgtcac gttaaccgga
     3961 aaagggaaaa agacatgctg aaacagcagg atatgacaga aaccgccaga gtggtgttta
     4021 atgaattaag c
//
LOCUS       DAJTNA010000270         3983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTNA010000270 DAJTNA010000000
VERSION     DAJTNA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.276"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            127..1176
                     /locus_tag="OO161_003967"
     CDS             127..1176
                     /locus_tag="OO161_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR5464832.1"
                     /translation="MANTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYEAKGRVLKQHVMA
                     PLIAYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKSHYQLVSTYSELSRQYVELQSEYKNLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKTAEMLQQIINASSRPGDQVADFFMGSGSTVKAALALGRRAIGVELET
                     GRFEQTVREVQDLIV"
     gene            1226..1301
                     /locus_tag="OO161_003968"
     tRNA            1226..1301
                     /locus_tag="OO161_003968"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1259..1261,aa:Ile,seq:cat)
     gene            1308..1383
                     /locus_tag="OO161_003969"
     tRNA            1308..1383
                     /locus_tag="OO161_003969"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1343..1345,aa:Arg,seq:tcg)
     gene            1399..1475
                     /locus_tag="OO161_003970"
     tRNA            1399..1475
                     /locus_tag="OO161_003970"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1433..1435,aa:Arg,seq:tct)
     gene            1978..3939
                     /locus_tag="OO161_003971"
                     /pseudo
     CDS             1978..3939
                     /locus_tag="OO161_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
BASE COUNT          960 a          956 c         1178 g          889 t
ORIGIN      
        1 gcggctggtg ggtgttaggg gcacccacca gccatctgct catgcgttgg ggtcacaagc
       61 aaacctcagg cccatctgct ttgcgcaaaa gcggtatgag cctatcagag aagtgcttat
      121 tgatctatgg ctaatactgt aaaaatatcc agttgtgagt taatcaacgc cgactgcctg
      181 gaatttatcc ggtcgttacc cgaaaattcg gttgacctga tagtcacgga cccgccgtac
      241 tttaaagtga agcctgaggg ctgggataac cagtggaagg gcgacgatga ttacctgaag
      301 tggctggacc agtgtctggc gcagttctgg cgggtgctga aacctgccgg aagtctttac
      361 ctgttctgtg gtcatcgcct ggcatctgat atcgaaatca tgatgcgtga acgcttcagt
      421 gtgctgaacc atattatctg ggcaaagccg tccggacgct ggaacgggtg caacaaggaa
      481 agcctgcggg cgtatttccc cgccacagag cgcattctgt tcgcggaaca ttatcagggg
      541 ccgtaccgcc cgaaagatga tggctatgag gcgaagggca gggtactgaa acagcatgtg
      601 atggccccgc tgattgctta ctttcgtgat gcgcgagctg ccctggggat aacggcaaaa
      661 cagattgcag atgccacagg aaagaaaaac atggtgtcgc actggttcag tgccagtcag
      721 tggcagctac cgaacgaaag cgattatctg aaattacagt cgctgtttgc ccgggtggca
      781 gaagagaaac atcagcgggg agaactggaa aagtcccatt accaactggt cagcacatac
      841 agtgagctga gccggcagta tgtggagctg cagagtgaat ataaaaattt gcggaggtat
      901 ttcggtgtga cggtgcaggt gccgtacacc gatgtgtgga cgtataaacc ggtgcagtac
      961 tatccaggga aacatccgtg cgaaaaaacg gcagaaatgt tgcagcagat aatcaacgcg
     1021 agcagtcgtc cgggagacca ggttgcagat ttttttatgg gctcaggttc aacggtaaaa
     1081 gcggcactgg cgctcgggcg tcgtgcgatt ggcgttgaac tggagaccgg acgttttgag
     1141 cagacagtca gggaagttca ggatttaatc gtttgaaacg gatgagattg cagaattaat
     1201 tacgcaccat tattattctg ctcccggccc tttagctcag tggtgagagc gagcgactca
     1261 taatcgccag gtcgctggtt caaatccagc aagggccacc atcacatacc gccattagct
     1321 catcaggaaa gagcgccagc cttcgaagct ggttgcgcgg agttcgggtc cccgaaggcg
     1381 gttcattatc tgtatcctgc gttgttagct cagccggaca gagcaattgc cttctaagca
     1441 atcggtcact ggttcgaata cagtagaacg cgccacactt attttccctg gctcgctttt
     1501 gcgggctttt tttaaatgtc tcacaattca ggcggttgac tgttgtctgg tttgcgggga
     1561 gtttgttaaa agaaactggc atggtgaatc cccctgtgcg gaggggcaat cagcaactgg
     1621 tgttttgtca ccgaccctta tcctttctgt gcgggttcag gtgctgatac tgaactcacc
     1681 gggaggcacc cggcaccatg caatggcaca tagcgccact ctccagcccc tctccggagg
     1741 ggctttctta tggacaaaaa aagcccgcgc tgggagacgc gggcggcaag gaataaacaa
     1801 caaaacgtga agtaatattt cagctggtga ataatatccg acagtaatca ctctgcgcaa
     1861 tagcgcggcc tttttcatat tgcgggctgt tgtctatctt ctgccattgt cctgtaactt
     1921 ccggacttca gcccgctcct cattttactc acaatattat ccaggccggg aggatttatg
     1981 gcatttaaac actatgacgt ggtcagggcg gcgtcgccgt cagaccttgc ggagcgactg
     2041 acacaaaaac tgaaggaggg gtggcagcca tttggcagtc cggtggccat cacgccttat
     2101 accctgatgc aggccattgc ggcggaaggt gatgtcacca cacctgtggt ggtgaagccg
     2161 tcggatggag aaggcgcagt tatcagcacc accagcaacc cggagtatta atttgttgtt
     2221 gccctggccg ggcagtccaa tgccatggct tacggtgaag ggcttccgct gccggagaca
     2281 tatgaccgtc cggacccgcg tattaaacag ctggcgcgtc gcagcactgt cacgccgggt
     2341 ggtgcgtcct gtaactacaa tgacattatt cctgcggacc actgcctgca tgatgttcag
     2401 gatttgagta agttttcaca cccgaaagcc agcgcagctc agtatggatg cgtggggcag
     2461 ggattacata tcgcgaagaa attgttgccg tttattccgg cgaatgccgg tattcttctg
     2521 gttccgtgct gccgtggtgg ttctgcattt ttggcgggcg atgaaggtac cttcagcgaa
     2581 tccaccggcg caagcgagac ctcggcacgc tggggtgtag ataagccact gtacaaggac
     2641 ctgcttaccc gtactcaggc cgcactgaag gctaacccta aaaatattct gcttgcagtg
     2701 gtctggatgc agggcgagtt tgatttgaaa cagggtgcat acgccactca gccggggctg
     2761 tttgattcca tggtggaaaa atatcgttct gacctgtcgg aattcggagg tcagtgtctc
     2821 gggggctctc cgtcatcggt tccctggatt tgtggcgaca cgacctacta ctggaagcag
     2881 acttattctt cgcaatacga tgcggtgtat ggtgcataca agacgaaatc cgcaaaaaaa
     2941 atcttctttg tgccgtttat gacggatgaa aacgggcgaa atgtgggtac caacgagccg
     3001 tcagaagatc cggatgttgc ggatattggg tattacggag ccggtggtcg aacggacgcc
     3061 aaaacctgga cgacggccga ccgtaaaacg cattttggat catgggcacg tcgtgggatt
     3121 atttccgacc gtctggcaac ggcgattctt gtgcatgccg ggagaaccgc tgaattcatt
     3181 accggaaaac agcctgatac ggtgaagccc accggacctt ccggtgaagg tacggagaga
     3241 gagccggaag ccccggtcag taaccgaacc ctgatgagtc tgctggcgtc cggcgaagac
     3301 ctggcatcac agggctggcg ctattatcac aaaccggcga gcggagacaa tgttaataaa
     3361 aacattgctg aagcggtggt cagtgatgcg ggggctacgg gaggtaaggc cctgcaactg
     3421 aataaaccgg aaaaccacat ctggtttctg gagcatgatg cagccggaca gggagtggag
     3481 ttactgaaga aggggggacg tgtgagcgta cggtttaagt tgccgggttc actgatgccg
     3541 aatcggtttg ccctgggcat ttactggcag ttgtcgtccc tgccggaggg agtgacgctg
     3601 gcagaggaag gcaacgacat gctgatgtcc ttcttcctgc agacggatgc gacgaacctg
     3661 aacgcgatgt accacaagaa gccgaatgcg aagctggata cgttcggggt ctttgataac
     3721 ggatggcaca cactggcttt tgagtttgcc ggaaacaaca gcattcaggt gacgccggta
     3781 ctggatgaga aacgggggac gccgttcaca ctggtgaaat ctccggcatc aggggcggcg
     3841 gacaaactgc aactgacagg catatcaaag gcggcgacat atacgctgct gattgacagt
     3901 gtgaaggtgg aagtgaacaa cgcggatgcc gcggcatgat aaaaaaagcc gccagcggca
     3961 ggaacggaag ctggcggagg taa
//
LOCUS       DAJTNA010000271         3915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTNA010000271 DAJTNA010000000
VERSION     DAJTNA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.277"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..2032)
                     /locus_tag="OO161_003972"
     CDS             complement(<1..2032)
                     /locus_tag="OO161_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5464833.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEFIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVWLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWTNNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAP"
     gene            complement(2048..2722)
                     /locus_tag="OO161_003973"
     CDS             complement(2048..2722)
                     /locus_tag="OO161_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5464834.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKIMPNKLPSNKES
                     IFYLNVLDIPPNSPKQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLINRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(2810..3352)
                     /locus_tag="OO161_003974"
                     /pseudo
     CDS             complement(2810..3352)
                     /locus_tag="OO161_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(3651..>3915)
                     /locus_tag="OO161_003975"
     CDS             complement(3651..>3915)
                     /locus_tag="OO161_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HCR5464835.1"
                     /translation="SGIIFLAYGLASPAFSSETATSTINGRISSPTCSMDVVNNHLQQ
                     RCGQLLQRVETNYRASSTAKGVTTEVVTVGNNDKRKIVLNRYD"
BASE COUNT         1147 a          922 c          720 g         1126 t
ORIGIN      
        1 ctggcgcatt catcacagca aacgtttcgg aaagacggtt cgccagatta acgccacccg
       61 accaggcgac aatgccccct gaaacactgg ctccagcctg tcgataagta ctcgactgac
      121 tataactgcc attcactgtc gcaaccggcg cgttccaggt caaattcgcc ccagctgtcg
      181 tttcatttcc ctgatgctga taactcaggt tgaccccata gttaaactga tcccggcttc
      241 caacggttcc tgataatccc gtattatttg aggcaacccc ctgatcatca aacgtcgttg
      301 agttagacat atatatttgc cgacgaggcg tcgtaacgtc atcaccccaa tcaaagggaa
      361 tcgatataaa aatattaaaa cgtttctctt catgatgatt ctcgtcatac gcctggcttg
      421 ccgcgagggt atagcttatc cgccgccagt tattggaata actcaactga taatccttac
      481 tgctgccgct acgcccccag taatctcgcc ataacgtact taaggacaca gacccccagc
      541 cttctggcaa tgactggctc atattggcag aaaagctatt tttacggcca aaatcgttct
      601 ggtaataatc ggcaatgtca tagatatcgt tttcatcacg gcgataatta tctttattgt
      661 ttgtccaaac gtgatcgtta aatgtccggt aatcacgcga cgaataacgc caggccgcca
      721 gaccaaaacg cgtcgacgtt tggctcacaa atttgttgta ggcaatttga taactttgcc
      781 cgtcaaacac atcgccgttg tcttgtttac tatgcgactt cgtggcatcg acggaaatgg
      841 caccaatgcg ggtattccag ccagtcccca aagtgaacgc gtaataatta ttcgcgacca
      901 tcgagccacc atacagcgtc aataaattat taaaaccata ctgataaccc gcctggacaa
      961 aatcactttg tttgctcgcc ccttcaatat ggctacgacc cgccgcaaaa tcatatttcg
     1021 atacgccggg ttgcagcata tttggcaccg ctgcataagg taccagatag gtggtaaccg
     1081 agccgtccgc ctctttcacg ctgacatcaa gatctgctcc accaccggcc aactgcaaat
     1141 ctgtaatcgc aaacgggcca ggaggaacct ctttctgata aacgacaaaa ccattctgtt
     1201 caatagttac cagcgcgtta ctctgagcaa tcccctgcac ccgtggcgta aaattttgtt
     1261 tcgagttagg caacatctgc atatcacgaa acaaccacac accgctgaag cgaacagaat
     1321 caaaaatatc gcttgatgtg tacatatcac ccacgcgaag cgtgccgaga agttgggcaa
     1381 atccacgttc cagatacagg gtattgcttt tccacacccc tggattgtta tttgttttac
     1441 tgaaactggc atcagaatgc agttgccacc ccagtaaatt taacccgctg ttaaaacgta
     1501 catatgtact cttgttatta cccgacgctt tatagtcgct gtaatactga ctcacataat
     1561 aagaggtata aaacgcatta ataccccgct cccagttttc cggtggaaca tagccacttt
     1621 ccagttcttc cacccaagcc tgcgggacac tgaaatcgag acgaaaaacc ccgatatccc
     1681 agctatagct cccaccctga acaagttgct caaatgttaa acattgctta ccactggcga
     1741 agttatcgct attaatgcct aaccgcttga taacttctct tgataaacat gtttcttgcg
     1801 ggttgtcttt aacaatgaac tcatatttcc cgcgccattg cttattgaca taaatatcga
     1861 tgtcatactg ccccggcaag ggttgattat catcaagacg aatatttgca acctgctggt
     1921 ctttcattcc acctatcata aaatgggtat caaaggtttc ttcagctgca taagcttcaa
     1981 tgccgagcaa taacgctacg attgctgatg caagtggggt cattctcaac ataggcagct
     2041 cctgcaatta aattttgtca ctaatatagt tgccgtggtc atcgataatg gtcagatacc
     2101 agttgtttgc attattactt ttgacattaa cactctgact ttctaagggg gcaatcataa
     2161 tagtttcata attgactttg acattattag ctttgacatc cgaaatcgtc acccaattag
     2221 ctgagtcatt ttttatcacc aatccattgc cactgcgatt tatcagtaac tttttaaagg
     2281 tcgctttatt caccggagca atacccgccg gccggtaaaa caatttgatt ctgttttgca
     2341 tcgcaaactt cagtgcattc ttaccttctt gttttggact attcggtgga atatccagaa
     2401 cattaagata aaaaatactt tccttattac tgggtaattt attaggcatg attttaattt
     2461 ttatttgttg cccagaattg gccgctactt tcactactgg tggcgttaac atgaaaggaa
     2521 cctgaatctt ttctggcggc agagacgtgt cgccatcatc aatccacgcc tgcatcagcg
     2581 aagagcggtt tccctgattc atcagttgta ccatgacctc tttctgctct gccgggtaaa
     2641 ttatacgggt cccgaaaata accattccgg catgcacggg gaatatcata gaagagatga
     2701 gtaaagataa taaccctttc atcttaatgc ctcttatatt tatgctggcc gccatgaata
     2761 tggcggccat tttaacttac tgaaaaaaca agatgattat tttaaatatt taatcctggg
     2821 caacacgcat aaccaatgaa gagttaacag taccagatgc aacaccactg tcagtcaact
     2881 tcagatattt tgccttaaac ggtattgcat attatccatc agcagtttta taactttcaa
     2941 tattaaatgg tgcggaaata tccagtacgt cactgctatc tcccaaatag agatagtatc
     3001 caactgtgtc attactactg gatattgcct tatcattacc aagaattttc tcgttggtga
     3061 tcaacgaaag ttttacgcca tcaatagtct tcggacaagt aattttcagc atactgctgg
     3121 ttacaccaac cacgccatca taagttgaat atttcgcttt accatcaaat acagtggtgg
     3181 gtacagtcgg catattaata ctgactgaat caccgccagc gacaaatgta catggtgatt
     3241 cagcaatatt acctttaatg gttaaagttc cggtatctac ttcagcagcg aaaataccag
     3301 cactcataaa tacagaagat aagacagcga caacaataat tgaatgtttc atgatgcatt
     3361 ccttaataag tttattttgt tttgttaaga gtctgtatct cttggaaaca aatttctcac
     3421 atcaaaaaag ttgccgcaat gtaaacatat tgttttgttg atttaaaaca aattaactaa
     3481 tgcgatataa atacaacaaa taattcgcta taacaaacat ttttgaaact gattaattaa
     3541 cgtaaattac cgtaaataac attcatgagg tgaataaaaa agccaaaatc attcatagga
     3601 atgattttgg ctttttaaat atgcttaatg cttaaaacgg tgttggcaaa ttaatcgtag
     3661 cgatttaaca cgattttgcg tttgtcgtta ttacccacag ttaccacttc tgtcgtaacg
     3721 ccttttgcag tagaagaagc acgatagttg gtttcaactc gttgtaacaa ctgcccacaa
     3781 cgttgctgaa gatgattatt aacaacatcc atactgcatg taggggaact aattctgcca
     3841 ttgatggttg atgttgcagt ctctgaagag aacgcgggag aagccaatcc ataggcaaga
     3901 aaaataattc ctgac
//
LOCUS       DAJTNA010000272         3821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTNA010000272 DAJTNA010000000
VERSION     DAJTNA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.278"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..464)
                     /locus_tag="OO161_003976"
     CDS             complement(<1..464)
                     /locus_tag="OO161_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5464836.1"
                     /translation="MPVLYNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFID"
     gene            689..1609
                     /gene="lpxL"
                     /locus_tag="OO161_003977"
     CDS             689..1609
                     /gene="lpxL"
                     /locus_tag="OO161_003977"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5464837.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            1781..3005
                     /gene="mdtG"
                     /locus_tag="OO161_003978"
                     /pseudo
     CDS             1781..3005
                     /gene="mdtG"
                     /locus_tag="OO161_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            3088..3462
                     /locus_tag="OO161_003979"
     CDS             3088..3462
                     /locus_tag="OO161_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5464838.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(3463..3690)
                     /locus_tag="OO161_003980"
     CDS             complement(3463..3690)
                     /locus_tag="OO161_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5464839.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
BASE COUNT          928 a          974 c          976 g          943 t
ORIGIN      
        1 atgtcgataa atagtgcatc gggatcgtca agcatggcgt tcacttccgc cgcttgcaga
       61 tactcaccaa cattgctggc atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga
      121 tcacgtacct tcatgcgcag tacccagaag gatttcccgt catcatccaa cgcgatattc
      181 aggcgtaaac cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca
      241 ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat gcgccagata cactcgccca
      301 aaaacattca gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt aggatcggcg
      361 atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt cgctctcagc caacattttg
      421 gcttttagcg cgtcgttgga aatgcggttg tataacactg gcatggtgta cggttcctgc
      481 gagatgggaa agtaaaaatc cgcggcatga tatagcaatt atcgataatt aacatccaca
      541 cattttacgc tacatttgcg cattaaaaat tatttgttat ttacaagcgc ggcaatttcg
      601 cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata catacaattg
      661 cccgaatagg ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact
      721 gcttcatccg cgttattggt taacctggtt gggtattggc gtactttggt tagtcgtgca
      781 attgccctac ccggttatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt
      841 tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag
      901 cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggcctgat
      961 ggaaaccggc atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat
     1021 cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat
     1081 ccattttctg acactggagc tgggtgcgcg gcagtttggt atgcaggaac cggggattgg
     1141 cgtttaccgc ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg
     1201 ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa
     1261 aggcgaagtg gtctggtacg caccggatca tgattacggc ccgcgctcaa gcgttttcgt
     1321 cccgttattt gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat
     1381 gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca
     1441 attgattatg ctgccgccag agtgttctcc gccactggat gatgccgaaa ctaccgccgc
     1501 gtggatgaac aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt
     1561 acaccgtcgc tttaaaacac gcccggaagg cgttccttca cgctattaaa tctcccctgc
     1621 cggatgcttc agaatggcat ccggcattac cacagcaaat ccccctgatt tagcgataaa
     1681 agctctctgg attgcgcccc ccgggagtcg ggcgcataat tagtgtgctt atcttttctt
     1741 cttatgttca ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtgaaaatga
     1801 cacccctata aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg
     1861 tgccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc ttggcgttac
     1921 cgatcactcc gccctgaata tggtccggta ttgtcttcag cattacattt ttattttcgg
     1981 ccatcgcctc accgttttgg ggtggactcg ccgaccgtaa aggccgaaaa ctcatgctat
     2041 tacgctctgc cctcggcatg ggcatcgtga tggtgttgat ggggctggca caaaatatct
     2101 ggcagttttt gatcctgcgg gcgcttcttg ggttacttgg cggatttgtc cccaacgcta
     2161 atgctcttat cgccacacaa gtaccgcgta ataaaagcgg ctgggcgctg ggtacgctct
     2221 ccacaggcgg cgttggtggt gcgttgctcg gcccaatggc tggcggcctg ctcgccgata
     2281 gctacggctt acgtccggta ttctttatta ccgccagtgt gctcatactc tgctttttcg
     2341 tcaccctgtt ttgcatcaga gaaaaattcc agccggtcag caaaaaagag atgctgcaca
     2401 tgcgggaagt ggtgacatca cttaaaaacc cgaaactggt actcagcctg tttgtcacta
     2461 cgttaatcat ccaggtggcg acgggctcaa ttgcccccat tctgacgctg tatgtccgcg
     2521 aactggcggg taacgtcagt aacgtcgcct ttatcagtgg catgatcgcc tcggtgccag
     2581 gcgtggcggc tctgctaagt gcaccacgac tcggcaaact tggcgatcga atcggacccg
     2641 aaaagatcct gattacagcg ctgatctttt ctgtactgct gttgatccca atgtcttacg
     2701 ttcagacacc attgcaactt gggattttac gttttttgct cggtgccgcc gatggtgcac
     2761 tactccccgc cgtacagaca ttgttggttt acaactcgag caaccagatc gccgggcgta
     2821 tcttcggcta taaccaatcg tttcgtgata ttggcaacgt taccggacca ttgatgggag
     2881 cagcgatttc agcgaactac ggtttcagag cggtatttct cgtcaccgct ggcgtagtgt
     2941 tattcaacgc agtctattca tggaacagtc tacgtcgtcg tcgaataccc caggtatcga
     3001 actgattttt cgcctttcat acttgcaaaa gcggagaatc agctatcctt ttccctgaaa
     3061 cctcatcaac tcaaagggag aatcgtgatg accatgtacg caacgcttga agaagccatt
     3121 gacgctgcac gcgaagaatt tcttgcagac aaccccggca tcgacgccga agatgcgaat
     3181 gtgcaacagt tcaatgccca aaaatacgtt ttgcaggacg gcgacatcat gtggcaagtt
     3241 gagttttttg ccgacgaagg ggaagaaggt gaatgtttac ctatgcttag cggtgaagcc
     3301 gcgcaaagtg tttttgatgg cgactatgat gagatagaga tacgccagga gtggcaggaa
     3361 gagaatacat tacatgaatg ggacgagggg gaatttcagc ttgagccacc gctggatacc
     3421 gaggaaggac gcgcagcagc tgatgagtgg gatgaacgtt aatcactcat acgggccatg
     3481 atgaatgtcg atcggcagca gtaaagtatc aaaaaccagg gagaatggca gatcaaggat
     3541 tgtgacataa cgccaggcgg agtcacgcac atcccattgc acaccgggat aatattggtt
     3601 gccatgcccc tgccccggta tagtgcgact aataatgctg ccacagccac tcagcaaggt
     3661 caccatgatg ctcaccacaa ttaaacgcat ttgatacctc ttccgtaaaa aaataccggc
     3721 ataacgccgt aatgccagtc agttaagcaa ctgactggct ctttttcggg gctgtggggt
     3781 atttccaggg cctctccttt accactctcg ggaaggccct t
//
LOCUS       DAJTNA010000273         3803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTNA010000273 DAJTNA010000000
VERSION     DAJTNA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.279"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(327..1724)
                     /gene="ccp"
                     /locus_tag="OO161_003981"
     CDS             complement(327..1724)
                     /gene="ccp"
                     /locus_tag="OO161_003981"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5464840.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFPREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2128..3777
                     /locus_tag="OO161_003982"
     CDS             2128..3777
                     /locus_tag="OO161_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5464841.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          987 a          964 c          892 g          960 t
ORIGIN      
        1 tcgcgcattt cgtgcagcgg aaatcgtttt gactccgcta tagtagaaat ggcctttgcg
       61 ccaaaacgtg aatcgagtag ttctgagcgg aaatccgtta acagcttctg gtccatttcg
      121 aactccttaa atttatttga aggcaataaa aaagtaggat ttatccgcaa tggaagcaag
      181 gcattacagg cttaatttaa ataacaaaat cctaagcaga aaaacgactt tttataatat
      241 aaacatttaa catgataata ttaaaaacag actttattat cgtaataaaa atcgcccaaa
      301 cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg cgtgtacacc
      361 ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg cagctctttg
      421 cctacctgat agcgcagcat cagtttcacc gccccgtcca gcgtcggcac gtcaccgcga
      481 tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg gcgcaattta
      541 tcacgctctt ctttagtcac attcatacga ccaatatccg ccgccgtaat ttccccaaag
      601 ttaaagtctt ttttcagccc caacggttca aaggaacgtc cgccgagaat aataccacca
      661 tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg ttgcgccgtc
      721 agcgcatttt catctccacg caaccattta tcaaatgggg aatccggcgt aattaatgtt
      781 ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg cggataaact
      841 tcgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat ttcgtcccag
      901 gatttcgacg ccatttcaat cgggttcaac ggcggtccac ctgcctgatc ctgcaatgtt
      961 gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac cgtcggcgcg
     1021 ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc gacgcccccc
     1081 gcattcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa acggggatcg
     1141 tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt ttgcgggatg
     1201 ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta atattccgcg
     1261 cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac cttacccgcc
     1321 cagtgtagcg cggtataacg cgttggtggc atagtttcat actgcatcac ccattcaatc
     1381 ttattcagat cgctttgcga aacgggttta tcagccagca gtgccgcacg cacggcctcg
     1441 aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt cgcgccagga
     1501 atataataat aggcgggtaa ttctgccgaa ggcgtgtggc aatagtcgca tcctttttcg
     1561 cggggaaagc ctaaaacctt attattttca ctgacagcag atgcctgaac atcggcctgt
     1621 ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata gcaaatcgcg
     1681 acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct tccctcacgg
     1741 tggtgagtta ttgtgaccca gaaaattttc cagaatgcag caaacgtctc tgcgagacaa
     1801 agccatcata aaaaacaggg agatacgctt tttgatagga atcaatcaat tacatgaata
     1861 taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca tatcatgatt
     1921 gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acaccaaatc cgtattttta
     1981 taaattccat tgatattagt gcgttatatt ttttgacaag ttaataaacg tgatccaccg
     2041 cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag ccgataatta
     2101 gcgacgaatt tcggaggttg gatccttatg ctcaatcaga aaattcaaaa ccctaatcca
     2161 gacgaactga tgatcgaagt cgatctctgc tatgaactgg acccgtatga attaaaactg
     2221 gatgagatga tcgaggcaga accggaaccc gagatgattg aagggctgcc cgcctctgat
     2281 gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc ggcgaaaatt
     2341 ttccccgaca gtaaaacctt tcccgactgc gcacccaaaa tggacccgct ggatatttta
     2401 atccgctacc gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt tgttgaaaat
     2461 cacttctggc tgccggaggt ctactccagc gagtatgtat cggacccgca aaattccctg
     2521 aaagagcata tcgaccagct gtggccggtg ctaacccgcg aaccacagga tcacattccg
     2581 tggtcttctc tgctggcgct accgcagtca tatattgtcc cgggcggccg ttttagcgaa
     2641 acctactatt gggattccta tttcaccatg ctggggctgg cggaaagtgg tcgggaagat
     2701 ttgctgaaat gcatggccga taacttcgcc tggatgatcg aaaactacgg tcacatcccc
     2761 aacggcaacc gcacctatta tttgagccgc tcgcaaccac cggtttttgc gctgatggtg
     2821 gagttgtttg aagaagatgg tgtacgcggt gcgcgccgct atctcgacca cctgaaaatg
     2881 gaatatgcct tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc ctatcgccat
     2941 gttgtgcgga tgccggacgg atcgctgctc aaccgttact gggacgatcg cgacacgccg
     3001 cgtgacgaat cctggcttga ggacgttgaa accgcgaaac attctggtcg cccgcccaac
     3061 gaggtgtacc gcgatttacg agcgggggcg gcctccggtt gggattactc ttcccgttgg
     3121 ctgcgtgata ctggtcgtct ggcgagtatt cgtaccaccc agttcatccc catcgatctg
     3181 aatgccttcc tgtttaaact ggagagcgcc atcgccaaca tctcggcgct gaaaggcgag
     3241 aaagagacag aagcactgtt ccgccagaag gccagtgccc gtcgcgatgc ggtaaaccgt
     3301 tacctctggg atgatgaaaa cggcatctac cgcgattacg actggcgacg cgaacaactg
     3361 gcgctgtttt ccgctgccgc cattgtgcca ctctatgtcg gtatggcgaa ccatgaacag
     3421 gccgatcgtc tggcaaatgc cgtgcgcagt cggttactga cacctggcgg gattctggca
     3481 agcgagtacg aaaccggtga acagtgggat aaacccaacg gctgggcacc gttacaatgg
     3541 atggcgattc agggatttaa aatgtacggc gatgaccttc tgggtgatga aatcgcgcga
     3601 agctggctga agacggtgaa tcagttctat ctggaacagc acaaactgat cgaaaaatac
     3661 catattgccg atggtgttcc acgcgaaggc ggcggtggcg agtatccgtt gcaggatggg
     3721 tttggctgga ctaacggtgt ggtacgccgt ttaattggtt tgtacggcga accataatat
     3781 ttttacagcc agccgctaac ttc
//
LOCUS       DAJTNA010000274         3764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTNA010000274 DAJTNA010000000
VERSION     DAJTNA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.280"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..587
                     /locus_tag="OO161_003983"
                     /pseudo
     CDS             1..587
                     /locus_tag="OO161_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(615..704)
                     /locus_tag="OO161_003984"
     tRNA            complement(615..704)
                     /locus_tag="OO161_003984"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(668..670),aa:Ser,seq:cga)
     gene            798..1595
                     /gene="mtfA"
                     /locus_tag="OO161_003985"
     CDS             798..1595
                     /gene="mtfA"
                     /locus_tag="OO161_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5464842.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1696..1771
                     /locus_tag="OO161_003986"
     tRNA            1696..1771
                     /locus_tag="OO161_003986"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1729..1731,aa:Asn,seq:gtt)
     gene            2085..3764
                     /locus_tag="OO161_003987"
                     /pseudo
     CDS             2085..3764
                     /locus_tag="OO161_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
BASE COUNT         1016 a          890 c          899 g          959 t
ORIGIN      
        1 tgtgcaggtg taagtaaatt caaggccaaa accgcgaacg atatgtaacc gacaaagaat
       61 accaggcagt attaagcgtt gcacctcttc ctgtttttat cgcaatggaa attgcctatc
      121 tgtgtgcagc gagggtttcc gatgtgttat cgctgaaatg ggagcagatt ggaaacgacg
      181 ggatctttat ccagcaaggg aaaacaggaa aaaaacagat aaaagcatgg agtccacgat
      241 tacaggcggc gatcgaaaaa gcaaaacagt taccaacatc cgcctatgta atcagcaatc
      301 aatacggcaa ccgatatatg tacaaaggct ttaacgaaat gtgggtagaa gcaagaaatc
      361 gcgcaggcaa aatttcaggt attttaaccg acttcacctt tcatgatctg aaggcgaaag
      421 gaatttcaga ctatgaagga agcagtcggg ataagcaact tttctctggt cacaaaaccg
      481 aggggcaagt gctaatctat gacaggaagg ttaaagtttc accgacactt gatgtcccgt
      541 tacctgaaaa tattccaagt aattccacgt gtgatttttg tcactgactt aatgatgtat
      601 aagtgattga attttggcgg agagaggggg atttgaaccc ccggtggagt tgcccccact
      661 ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag atggttgcca
      721 tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg
      781 agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca gcacatcaaa
      841 ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg acggaacagg
      901 aacaaagcaa attagtcact cttgccgaac gttttttaca gcaaaagcgg cttgttcctt
      961 tacagggctt tgaactggat tcattaagaa gctgccggat agcacttcta ttttgcctac
     1021 ccgttctgga gttaggactg gaatggctgg atggttttca tgaagtctta atttatcctg
     1081 cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat aaccaacgta
     1141 ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg ttggatatac
     1201 aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct cataagctgg
     1261 acacccgtaa cggcgatcgc gccagcggag ttccctttat tccgttgcgt gaggttgctg
     1321 gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc gaattggttg
     1381 gcgagaatgc ggcgagcatt gatgcttatg cagccagcga tcctgctgaa tgttttgccg
     1441 tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc ccttcattgt
     1501 ggcaacgttt ctgccaattt tatcaacaag atcctttgca gagactgcat cacgctaatg
     1561 atacagactc gttttcggcg acgaatgttc attaattaac aactttgcag attaattaac
     1621 caattgaaat gacttatgaa atttagtgtt gacagacaag gtaccgctaa gtaatatgcg
     1681 ccccgttcac acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat
     1741 gtcactggtt cgagtccagt cagaggagcc aaattctaaa aattcgcttt tttagcgcaa
     1801 tgtcactgac cttagttgaa cattgctttt taacggatag cgggttttta acatcttaag
     1861 cgccctcgac ctttatggtt gagggcgttt tgctatgaac gccatcacca ttttcccctc
     1921 gattataaaa cttgagttat tcagtagtct cccctcttgc aactcacacc caaaactgcc
     1981 taacgaaaag ttattaattt tcaatcatat tgctatcagt atttacattt tttcgctgtg
     2041 ctagaaaggg cgcatttatg ttagctcgtt cagggaaggt aagcatggct acgaagaaga
     2101 gaagtggaga agaaataaat gaccgacaaa tattatgcgg gatgggaatt aaactacgcc
     2161 gcttaactgc gggtatctgt ctgataactc aacttgcgtt ccctatggct gcggcagcac
     2221 aaggtgtggt aaacgccgca acccaacaac cagttcctgc acaaattgcc attgcaaatg
     2281 ccaatacggt gccctacacc cttggagcgc tggaatcggc ccaaagcgtt gccgaacgtt
     2341 tcggtatttc ggtggctgag ttacgcaaac tcaaccagtt tcgtacgttt gctcgaggtt
     2401 ttaataatgt ccgccagggt gatgaactgg atgtcccggc acaagttagt gaaaaaaaat
     2461 taaccccgcc gccgggtaat agcagtgaca acctcgagca acagatagcc agtacttcac
     2521 agcaaatcgg gtctctgctc gccgaagata tgaacagcga gcaagcggca aatatggcgc
     2581 gtggatgggc ctcttctcag gcttcaggcg caatgacaga ctggttaagc cgcttcggta
     2641 ccgcaagaat cacgctgggc gtggatgaag attttagcct gaagaactcc cagttcgatt
     2701 ttctccatcc gtggtatgaa acgcctgata atctcttttt cagtcagcat actctccatc
     2761 gtactgacga gcgtacgcag attaacaacg gcttaggttg gcgtcatttc actcccacat
     2821 ggatgtcagg catcaacttc tttttcgacc acgatcttag ccgttaccac tcccgcgccg
     2881 gcattggcgc ggagtactgg cgcgactatc taaaattaag cagtaacggc tatttgcgac
     2941 tgaccaactg gcgcagcgca cctgaactgg acaacgatta tgaagcacgc ccggccaatg
     3001 gctgggatgt acgcgcagaa agctggctac ccgcctggcc gcaccttggc ggtaaactgg
     3061 tctatgaaca gtattatggc gatgaagtgg ccctgttcga taaagacgat cggcaaagta
     3121 atcctcatgc cataaccgct ggacttaact ataccccctt cccgctgatg accttcagcg
     3181 cggagcaacg ccagggtaaa cagggcgaaa atgacacccg ttttgccgtc gattttacct
     3241 ggcaacctgg cagcgcaatg cagaaacagc ttgacccgaa tgaagtcgct gcacggcgta
     3301 gccttgcagg cagccgttat gatctggtgg atcgcaacaa caatatcgtt ctggaatatc
     3361 gcaaaaaaga actggttcgc ctgcccctga cagaccccgt gacagggaag tcaggagaag
     3421 tgaaatcact ggtttcgtcg ctacaaacca aatatgccct gaaaggctat aacgtcgaag
     3481 ccaccgcact ggaagctgcc ggtggcaaag tggtcacaac gggtaaagat attctggtta
     3541 ccctgccggc ttaccggttc accagtacgc cagaaaccga taacacctgg ccgattgaag
     3601 tcaccgctga agatgtcaaa ggcaatttgt tcgaatcgtg aacagagcat ggtagtcgtt
     3661 caggcaccta cgttaagcca gaaagattcc tcggtatcgt taagtaccca gacgttgagc
     3721 gcggattccc attcaaccgc cacactgact tttattgcgc atga
//
LOCUS       DAJTNA010000275         3652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTNA010000275 DAJTNA010000000
VERSION     DAJTNA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.281"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(739..2211)
                     /locus_tag="OO161_003988"
                     /pseudo
     CDS             complement(739..2211)
                     /locus_tag="OO161_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            complement(2362..2961)
                     /locus_tag="OO161_003989"
     CDS             complement(2362..2961)
                     /locus_tag="OO161_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5464843.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDLAYEGSGSGDWRTDAFIVGIGYRF"
     gene            complement(3029..>3652)
                     /locus_tag="OO161_003990"
     CDS             complement(3029..>3652)
                     /locus_tag="OO161_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464844.1"
                     /translation="MNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNA
                     NSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYD
                     DQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVI
                     DMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          739 a         1032 c          929 g          952 t
ORIGIN      
        1 aaaatgaata attaacgttg atgcacctct aatagttgcg ttataggctc cgctactcca
       61 gttccatcca actgctttat cattcgcaac ggtgcttcct gttttcccta aggcaaacgc
      121 accaacatga cttgctttta atgtgatatc ggaggaacca tcaaaagcca cattgcttat
      181 tttcctggca gtttttaatt ttgcagctgt agaagcattg ccggatagtt caccagaaag
      241 gccaccgctg aatgtttgtc gattagtcca ggtattcgct gtactgagta acggtatttt
      301 ctccccgctt gtgccgagtt ctcgtaaacc gaggtattcg ataacggcag caacagtcga
      361 tttcgcaaga atatcccgcc cgacttttgt cagggttgcc aggctggcga catcattccc
      421 cgtaaaatac ggaaacttgt ctgccgcagt agtaagcccg gccagcgccg tcagggtggc
      481 atctttcggt tgcttacccg caagcgcgtt agtcatggtg gtcgcaaaat tcgggtcgtt
      541 tcccagcgcc gccgctaact cgttcagcgt attcagtgcg tcaggtgacg aatctacaag
      601 ggcggcaatc gcagccataa cgaaagccgt gcttgcgatt tggatattat tcgttccctg
      661 tcgcgcagtt ggtgttgttg gcgttccggt cagtgcagga ctatttaagg gcgctttctt
      721 gttcgtttca cccattaccg ccttaaccgc ttttggcgtt gccgccagtg actcggaagt
      781 gctgttggtc gcactgctga gctgtactat cccctttttc gtcgtgctcg catcctccag
      841 cgccacggcg gatgcaatat cctctgcccg ttttgctgct gtctcggcgc gcgttgccgc
      901 ggattccgcc gtacttttgc tctgagctgc cgccgtcgca ctaccagctg cctctgatgc
      961 tttcattgtt gctgtcgtgg cactaccttt cgctgctgac gcttgtctgg tcgcctcatc
     1021 ttttgaagca gacgcagatg atgccgatga cgccgctgaa ctggctgacg atgcggcagc
     1081 cgccttagag gaagcagcat tgtctgctga agtctttgca tttgtttcag aggtttttgc
     1141 tgcagaagca gacctcgctg ctgcagtggc ttgctcagtg gctttgccag ccttcgttgt
     1201 ggctgttgaa gcggatgatg cggcgctttc tgccgatttt ccggcggcgc tagcactggc
     1261 tgaggcctgc ccggcacttg ttgacgcggc actggcagac gacgcagccg ctgtttttga
     1321 gcctgccgca gctgaggcgc tctgtcccgc tgctgtttca gaagacttag cgttcgtctc
     1381 ggacgttttt gccgccttcg cggaatttcc tgccaccgtt gccgaggaag ctgcactact
     1441 ggcacttgat gatgcattcg tttctgaaga tttcgctgcc tcttttgaag ccgccgcacc
     1501 ccgtgccgag gtggcagctt ctgacgcctt cgtggtcgct gtggatgcag aagtggctgc
     1561 cgatttttgt gatgctgcgg cattcgtttc tgacgttttc gcggcactgg cgctggtagc
     1621 tgccgcgctt tttgatgact ctgcagcagc agcacttttt gctgcttcac gggcctttgt
     1681 cgatgccgtt cctgcgctgg aagacgctga ctgagccgac gacgcggcct gtccggctga
     1741 cgtgcttgcg gcacgtgctg aggctgcagc atcggttgca tgagttgccg cctcgctggc
     1801 tgatgcactg gcatcgctgg ctgatttttt cgcggctgcc gtattctgtg caaccgcgga
     1861 ggcgttacgt gacacctctt ccaccatctg ctcaaagcgg cgcagtgcct ccggtcggac
     1921 atcatcctcc gtcatggcac cgagaaaatc atttagcgta cctggtctgg aaccttcata
     1981 gacggtaatg gtcccggcat gtgaaggcgg aaaaccttca accagcaggg tgacgctgta
     2041 ctgaccatgc tcaacatcca tgctgtaacg tccggcttca tccggatttt cagaggccac
     2101 cgtgttcacc accaccgtgc tgctggttcg tctggccttc agcacaatgg tgcagttctg
     2161 tactggtttt cctgtgccat ctttaagcac gccagaaatt tttactgtca tactttccac
     2221 caataaaaaa agcccgcagc agtgacgcca cgggcttcag gacagtgtaa ctttacgttt
     2281 cctcaaacgc agttcacccc ataaggtgga tgaacctgcg tatcataaca atatttacag
     2341 aagataaatc ggcgtctgtt gtcagaaacg gtatccgata ccaacaataa atgcatccgt
     2401 tcgccagtcg ccactaccgg aaccttcata agcaaggtca acggccacgg attcggtcgg
     2461 gttaaactgc acgccagccc cccacgccag agaggtattg ctgtggcgac cgtcatcact
     2521 tccggtcagc acgtcgtgcg ttttcccctt gttgtcagtt acgcggagat aatccccgga
     2581 gaaagtcgac acacggctgt aagccacacc cgccatcgca tacgcgctga accattcatt
     2641 cacgcgtaca gacggccccg ccatcacact gaaccagcgg ttacgcacgg aatcttcatg
     2701 ccagcgggta tcgctgtagc gcgttttttg ctcatcctca gcattggcat aactgaagga
     2761 cgtaatcagc cccagcgcgt ccgtaaactc ataacggtat ttcacgttaa tcccgttcag
     2821 atcatcacta ccgggaacgt tcgtcgaggc atggagatac cccgcgctca gcgtggactg
     2881 atgttctgct gcactcgctg gcgtagcagc ggcgacctgc cagactactg cggacaaaat
     2941 aacagcacat aatttacgca taattacctc tcgcttttct gcaataaaaa aggcgccatt
     3001 tctggcgccc gtatctgggt tataaaattc agctaatcgt gatgcctgca gtggctttct
     3061 tcatcaccac aaccagcaaa tcgctgatac ttgctgtggg ataccagtta tttaccagcc
     3121 atgctgacac cgaaaactcc agtgtcatgt gaccgtgacc ggcaggcata tcaataacac
     3181 cactgtaaat cagcgtatta tccagcgcgg tacggttata aatttcagca ccgtttttcc
     3241 gcactatcag acggcatgag gagtaaatat cagtatgctc tttctcatgt ttagcgccgc
     3301 tgaatgccac cgccggaata acaatctgcc ggtcaaacgg ctgatcgtca taaaccctga
     3361 cggtaatggt tcctgatggc caccgctccg gtgcacggga gtcccggggg aaagctttgc
     3421 ccactgtttt aacgagatcg ccttcaatct ggttcgcgga caattttccc agaacccgac
     3481 agttctcgtt aatcgtgacg ttgttgagcg tcccggagtt cgcattcacg ttaccgctga
     3541 tatcggcatt tttcgccgtc agccgcccgt ccggtgtcag ggaaaatgcc ggaggattac
     3601 cgccgctggt aatggtggga gccgtcagat atttcaggaa cacttcattc at
//
LOCUS       DAJTNA010000276         3613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTNA010000276 DAJTNA010000000
VERSION     DAJTNA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.282"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..1046)
                     /locus_tag="OO161_003991"
     CDS             complement(<1..1046)
                     /locus_tag="OO161_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5464845.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1647
                     /locus_tag="OO161_003992"
     CDS             1537..1647
                     /locus_tag="OO161_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5464846.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            1927..2922
                     /gene="pgl"
                     /locus_tag="OO161_003993"
     CDS             1927..2922
                     /gene="pgl"
                     /locus_tag="OO161_003993"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5464847.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2963..>3613)
                     /locus_tag="OO161_003994"
     CDS             complement(2963..>3613)
                     /locus_tag="OO161_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5464848.1"
                     /translation="TAVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDF
                     GINMNNVTNSSIDFTPLVNEPFVLACRRDHPLAKKQLVEWQELVGCKMIGVRSSSGNR
                     LLIEQQLADKPWKLDWFYEVHHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLV
                     EPVIRRTLGIIRRKDAVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          955 a          739 c          873 g         1046 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctatcc tggtaagtat atttgctttt tttgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atatcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaacac cgatctacga cgtaatgcgt tgaactgtgc aggatatgag
     1621 ctgattgttg agcgaatggg agcttgattg gaagttgtta gggtatggtg tttacggtcg
     1681 ttgatatggc gaacaaacgc ggggagcgtc acgctcccta aatggtcgcg aaaatatctg
     1741 tggcattgtc aaatttgtga cggtgattaa tattttaatg atatgccatt cgaaatggtg
     1801 tgctaacgta ttgatttaat tgtgacgcga tgttgtattt tatcgcgttt tgttgtctgt
     1861 ttttcattcg cttgatgttg ctatttacat gaaaaataaa gaatttattg tttttgaggt
     1921 gtgtttatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
     1981 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
     2041 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
     2101 cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct
     2161 gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
     2221 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
     2281 gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
     2341 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
     2401 gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccattgaagg ggccggcccg
     2461 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
     2521 tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg
     2581 gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg
     2641 gatggtcgcc atttatacgc ctgcgaccgt accgccagcc tgattaccgt tttcagcgtt
     2701 tcggaagatg gcagcgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
     2761 ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa atctcacctc
     2821 atctcggtat acgaaattgt tggcgagcag ggactactgc atgaaaaagg ccgttatgcg
     2881 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg
     2941 cagtctctcc ttcgccggtg tattaaccta tctcctgtaa cgcatgtctc tggcgttcga
     3001 cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggcaaaaa
     3061 agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac
     3121 gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg
     3181 ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa
     3241 gatgatgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta
     3301 tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgcaaccc actaactcct
     3361 gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggcc agcacgaacg
     3421 gctcgttgac cagtggggta aaatcaatgg atgaatttgt gacgttgttc atgtttatgc
     3481 caaagtctga ttcgttacac agcaccgatt ccatgcaatt attcgtgcct tgttccagaa
     3541 tccgcacttt aatattggga tacagctcat taaatttacc gattgccagc ggtaaaaaat
     3601 aaaatactgc ggt
//
LOCUS       DAJTNA010000277         3591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTNA010000277 DAJTNA010000000
VERSION     DAJTNA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.283"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            175..474
                     /locus_tag="OO161_003995"
     CDS             175..474
                     /locus_tag="OO161_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5464849.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            569..1480
                     /gene="glyQ"
                     /locus_tag="OO161_003996"
     CDS             569..1480
                     /gene="glyQ"
                     /locus_tag="OO161_003996"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5464850.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            1490..3559
                     /gene="glyS"
                     /locus_tag="OO161_003997"
     CDS             1490..3559
                     /gene="glyS"
                     /locus_tag="OO161_003997"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5464851.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILEKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT          853 a          935 c          991 g          812 t
ORIGIN      
        1 ctgtgtctct gtatctggtt aaaaaaagtc tgaccgataa atcatttggt tggcgcactg
       61 gaataatctg agttttatca ctacagctta tagaggctta aggaaattcg taagatatca
      121 gccactatac cgatataaat aataagactc acctgcaaac cagacggtaa tttaatgatg
      181 atgaacgctt tctttccggc aatggcgctt atggtgctag tgggttgttc tacaccgtca
      241 cccgtgcaga aagcacaacg ggtaaaggtt gatcctctgc gttcgttgaa tatggaagcg
      301 ttatgcaagg atcaggcggc aaaacgttat aacaccggcg agcaaaaaat cgacgtcacc
      361 gccttcgaac agttccaggg aagctatgaa atgcgcggtt ataccttccg taaagagcag
      421 tttgtctgtt cttttgacgc agatggccat tttttgcatc tttccatgcg ttaagccctg
      481 ctttttcccg tttcgtactg tatatcttcc atccagcggg tatactgatc ccttccttta
      541 aatccacacg tatccagcac gaaataatat gcaaaagttt gataccagga ccttccaggg
      601 cttgatcctg accttacagg attactgggc tcgccagggc tgcaccattg ttcaaccatt
      661 ggacatggaa gtcggcgcgg gaacctctca cccaatgacc tgtctgcgcg cgctggggcc
      721 agaaccgatg gcggctgctt atgttcagcc ttctcgtcgc ccgaccgatg gtcgctacgg
      781 cgaaaacccc aaccgtttac agcactacta tcagttccag gtggtcatta agccatcgcc
      841 ggacaatatt caggagctgt acctcggttc tctgaaagag ctgggcatgg acccgactat
      901 tcacgacatc cgtttcgtgg aagataactg ggaaaacccg actctgggtg cctggggact
      961 gggctgggaa gtgtggctaa acggcatgga agtgacgcag ttcacttact tccagcaggt
     1021 tggtggtctg gagtgtaaac cggttaccgg cgagatcacc tacggtctgg aacgtctggc
     1081 catgtacatt cagggcgtag acagcgttta cgacctggtc tggagcgacg gcccgctggg
     1141 taaaaccacc tacggcgacg tgttccatca gaacgaagtg gagcagtcca cttacaactt
     1201 cgaatacgcg gatgtggact tcctgttcac ctgctttgag cagtacgaga aagaagcgca
     1261 gcagctgctg gcgctggaaa atccgctgcc gctgccagcc tacgagcgta ttctgaaagc
     1321 cgcccacagc ttcaacctgc tggatgcgcg taaagccatc tccgtcaccg agcgtcagcg
     1381 ctacattctg cgcattcgca ccctgaccaa agcagtggca gaagcatact acgcttcccg
     1441 tgaagccctc ggcttcccga tgtgcaacaa agataagtaa gaggcggcta tgtctgagaa
     1501 aacttttctg gtggaaatcg gcactgaaga gctgccacca aaagcactgc gcagcctggc
     1561 tgagtccttt gctgcgaatt ttactgtgga gctggataac gctggcctcg cacacggcac
     1621 cgttcaatgg tttgctgctc cgcgtcgtct ggcgctgaaa gtagctaacc tggcggaagc
     1681 gcaaccggat cgtgaaatcg aaaaacgcgg cccggcgatt gcccaggcgt tcgacgctga
     1741 aggcaaaccg agcaaagcgg cagaaggttg ggcgcgtggt tgcggtatta ccgttgacca
     1801 ggctgagcgt ctgactaccg ataaaggcga atggctgctg tatcgcgccc atgtgaaggg
     1861 cgaaagcacc gaagcactgc tgccgaatat ggttgcgact tctctggcga aactgccgat
     1921 cccgaaactg atgcgttggg gcgcaagcga cgtgcacttc gtgcgtccgg tgcacaccgt
     1981 aaccctgctg ctgggcgaca aagtcattcc ggcaactatt ctgggcattc agtccgatcg
     2041 cgtgattcgc ggccaccgct ttatgggcga accggaattc accatcgaca acgccgatca
     2101 gtatccggaa attctgcgtg agcgcgggaa agtcatcgcc gattacgaag aacgtaaagc
     2161 gaagattaaa gccgatgccg aagaagcagc gcgtaagatt ggcggtaacg ctgacttaag
     2221 cgaaagcctg ctggaagaag tggcttcgct ggtggagtgg ccggtcgttc tgaccgcaaa
     2281 attcgaagag aaattcctcg cggtgccggc tgaagccctg gtttacacca tgaaaggtga
     2341 ccagaaatac ttcccggtgt atgcgaacga tggcaaactg ctgccgaact tcatcttcgt
     2401 tgccaacatc gaatcgaaag atccgcagca aattatctct ggtaacgaga aagtcgttcg
     2461 tccacgtctg gcggatgccg agttcttctt caataccgac cgtaaaaaac gtctggaaga
     2521 taacctgccg cgcctgcaaa ccgtgctgtt ccagcaacag ctgggtacgc tgcgtgacaa
     2581 aactgaccgc atccaggcgc tggctggctg gattgctgaa cagattggcg ctgacgttaa
     2641 ccacgcaacc cgtgcgggcc tgctgtctaa gtgtgacctg atgaccaaca tggtcttcga
     2701 gttcaccgac acccaaggcg ttatggggat gcactacgcg cgtcacgatg gcgaagcgga
     2761 agacgtagcc gtcgcgctga atgagcagta tcagccgcgc tttgccggtg atgacctgcc
     2821 gtccaacccg gtagcttgtg cgctggcgat tgctgataag atggatactc tggcgggtat
     2881 cttcggtatc ggtcagcatc cgaaaggcga caaagacccg tttgcgctgc gtcgtgccgc
     2941 acttggcgtg ctgcgaatta tcgttgagaa gaacctcaac cttgatctgc aaacgctgac
     3001 cgaagaagca gtgcgtctgt atggcgataa gctgactaac gccaacgtgg ttgatgatgt
     3061 tatcgacttt atgctcggtc gcttccgcgc ctggtatcag gacgaaggtt acaccgttga
     3121 caccatccag gcggtactgg cgcgtcgtcc gactcgtccg gctgatttcg atgcccgtat
     3181 gaaagcggta tcgcatttcc gtaccctgga tgcagctgct gcactggcgg cggcgaacaa
     3241 acgtgtatct aacattctgg agaaatctga cgaagtgctg agcgaccgcg tgaatgcctc
     3301 taccctgaaa gagccggaag aaattaaact ggcgatgcag gttgtggtgc tacgtgacaa
     3361 gctggagccg tactttgcgg aaggtcgtta ccaggatgcg ctggtcgaac tggcagagct
     3421 gcgtgagccg gttgatgctt tcttcgataa agtgatggtc atggttgatg acaaagaatt
     3481 gcgtctcaac cgtctgacca tgctggagaa actgcgcgaa ctgttcctgc gcgttgcgga
     3541 tatttcgctg ttgcagtaat aacgccgtta ttaaaaagcc tgccatctgg c
//
LOCUS       DAJTNA010000278         3582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTNA010000278 DAJTNA010000000
VERSION     DAJTNA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.284"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(296..991)
                     /locus_tag="OO161_003998"
     CDS             complement(296..991)
                     /locus_tag="OO161_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5464852.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(984..2411)
                     /locus_tag="OO161_003999"
     CDS             complement(984..2411)
                     /locus_tag="OO161_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5464853.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVVETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHLGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2422..3141)
                     /locus_tag="OO161_004000"
     CDS             complement(2422..3141)
                     /locus_tag="OO161_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5464854.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVGHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          922 a          977 c          740 g          943 t
ORIGIN      
        1 tttgccttgt caaaaaaatg gtagatgaat accaacaaaa ctctgggcag taataaacaa
       61 cattctgata gagagtgcct gtataaagct gaagtgacaa tgaccgggaa gggaacccgt
      121 tttcaggaat ggatgcctgt ctttttcact gtaaatatcc tagcattact aatgccagga
      181 tatttacaga agagcagcat aaccatgacg gaacggggtt aagtcaggtt gtccttaacg
      241 ctattttcaa cctcgtatgc cttcttcagg tttatgttca gatctcatat ctctctcagc
      301 aatcctcaat aatcagatac accgctttca ccgggccatg aaccccgacg actttgataa
      361 gctcaatatc cgccgttgaa ctagggccgc taatgatgtt aatgcaggac ggcattcgtt
      421 caccggcctg cgctttctga tgcaattttt ctgcgagttg cgctacacgc ggcaggattg
      481 tgcttttacg caggataaaa agagaagatt ccgggagcag gctcaatgaa cgcccgcgct
      541 cggcggcgga aaaaagaacc acgcctcccg attcggttaa accatattca gcatacacaa
      601 cacccacttt agcctgctct gcctgcgaga tattctcggc acctttcgcc ggatcccaaa
      661 caacggcatt gcattcctgc tgcaaacgtt cactaatccc caattcctcc agcctcgtgt
      721 caccgctaat cacgaccgac tgatctccca gctctttaca cagacgtatt gcagcttctg
      781 ccgccttcgc ctcgctggtc agctcacagc gcgtcaacat aacatcgctg gcaaactgaa
      841 taaacgcgtc acagcgctgc tgttggttaa gttgggtaag ccgctcgtta gcatagttgt
      901 taagcggcgc atcttctgct tgcggttcaa gtcgcagcgg gcgacccagt gcctgagcaa
      961 cgttattcaa aaattcgcct cgattatcca ttctttttct cctgcgcctg atgtttctta
     1021 aaccaactac ggaaactctc tccgtcagct tcaggaagat cgcgtgcttc catccagtcg
     1081 ctaatcgcgc caaatttgag tggtgttttg ccgccattga taaaccagct tgccgcatga
     1141 gcgccggcca tcatcccgac tttccacaat cctagatgac tattggcata agcgaacatt
     1201 tttatcgccc gttgctctgc ttttgcggtg atcccttttt cagccatcac ccgacgatga
     1261 cgcaaaatca gttttgacag cggaatacgc accggacaca cgctgtcaca agcagtgcat
     1321 aaagagcagg cgtagggtaa atctttaaaa tctttatagc cgccaagtag cggagaaatc
     1381 accgcaccaa ttggccctgg ataaatagag ccatatccat gaccgccaat atggcgatat
     1441 gccggacaag tattcataca agccccgcag cgaatacagc gcagcacatc ccgaaattca
     1501 gaggccagca cctcagaacg cccgttatcg acaataacca gatgaaactc ttcaggacca
     1561 tcaacgtgcc ccgcttcgcg cggtcctgtc agccaggtgt tgtatcccgt caaacgtgca
     1621 ccaacggcac tgcgcgccag catggtgatc aatacatcta cctcggcaaa cgtgggggca
     1681 atacgctcca ttcccatcac tgcaatatgc gttttaggca gcgtggtaca cattcgcgca
     1741 ttaccttcat tggtcaccag gcataccgaa cctgtctctg ccaccgcgaa attacagccg
     1801 gtaataccta tttcagcact gaggaaatct tcgcggattt tttgccggat gaataaggtc
     1861 atcgcttcag gcgtttccgg cccctcatag cccagacgtt cgtgtagcac tcgacggatc
     1921 tgatggcgat ctttatgaat tgccgggacc acaacatgag atggcggatc ttgatccagc
     1981 tggagaatat attcacccag atcggtttca accacctgaa tgccagcatc ctgcaacaca
     2041 tgattgacac caatctcttc ggtcaccatc gatttagatt tcaccacctt ccgggcattt
     2101 ttgcgttggg caacctgtaa aatgtagcgg gtagcgtctt ctttgttttt tgcaaaccag
     2161 acgtgaccgc cgttttgcgt cactttttct gagagctggt acagataagc gtcgagatta
     2221 ctcagaacat gatcacgtat ctgggcggcc cgatcgcgcc actcctccca gtgccccaat
     2281 tcatcgacca ttttttgccg atttgcccca atacgctgct gcgcgtttgc caccgctttg
     2341 cgcatgatcg gatcttcaat ttgctgacgg atgcgtgtct taaaatctgt attactggtt
     2401 ttgatcgaca tctttatatc ctcagcggct catcaacact tcagcaatat gcatcacttt
     2461 gactttctgc ccttcccgtt gtaatcgccc actgatgttc agcaggcaac tcacgtcagc
     2521 accaattaaa tactcagggc ggacttccat caggtgccca accttttctt tcaccatctc
     2581 gccggatatt tcggccattt tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg
     2641 ttcagcaaag gtcaacagct ccagtccacg cacatttttc agcagcgtaa gtggctcgtc
     2701 cttcactccc agcttacggg ccaggctaca agatgggtga tacacagctc tcccttgcaa
     2761 actggcacct acatcgacta cccctaattt attaacaata aaagaggtga gatcctgcat
     2821 acgcgcggca accttttcgg cacgtgatgc ccattcaggt tcatccgcca gatacgtcgg
     2881 gtaacttttt acggcatagg tgcaagagcc agccggtgaa ataatgggat cgtcgttatc
     2941 ctccagtgcg gcgatcagat ttttcatccc tggaatcgct tctttgatat aaccgctatt
     3001 gatcgcaggc tgaccgcagc atccctgttt ctccgggaaa tttacgcgac agccgagttt
     3061 ttccagtagc agcacggagt ctcgtgccat tcttgatttc agggcgtcac caatacaggt
     3121 gacaaagaaa ttgacattca caactaatac tccattactt catgcccatt tatgcccgat
     3181 gtttcccccc caccggggga tcaatcatca ataaatatgg cgttcttttt taattggcta
     3241 gcaacatact acccctcatt tgtatgacaa atgataatct ttcacccaaa cgtcaaatgc
     3301 aataactaat tttatttctt tcggcatcgc tcacatttta tggagaaatc gacgatcatt
     3361 tttggcataa gaaatccatt taaaaatggc tacatccctt atacctattc agagctactt
     3421 attcagggat tattgagata tacactgatg gtgtacaaac aaaatcaccg acaataatca
     3481 cgtatcaagt aatagtcggg attttattta attgaagcaa tttagtttgt atcttttatt
     3541 taattactaa gccagaccac ttcaaaaaaa catataaaaa ag
//
LOCUS       DAJTNA010000279         3567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTNA010000279 DAJTNA010000000
VERSION     DAJTNA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.285"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..357)
                     /locus_tag="OO161_004001"
     CDS             complement(<1..357)
                     /locus_tag="OO161_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5464855.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGG"
     gene            complement(700..2154)
                     /gene="amn"
                     /locus_tag="OO161_004002"
     CDS             complement(700..2154)
                     /gene="amn"
                     /locus_tag="OO161_004002"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5464856.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGFFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(2256..3567)
                     /gene="shiA"
                     /locus_tag="OO161_004003"
                     /pseudo
     CDS             complement(2256..3567)
                     /gene="shiA"
                     /locus_tag="OO161_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate transporter"
BASE COUNT         1004 a          851 c          831 g          881 t
ORIGIN      
        1 gccgcctttt ttattgaaaa ttgtgcgaac gttagcaatc gtacggttaa catttgaagt
       61 caatgtctcg gcgataatca ttgagccatt aggaccaaag ccttcataac gtccctgcac
      121 gaacgtttca tctccgccgc ctttggcttt atcaattgct ttatcaataa cgtgttttgg
      181 aacttgtgcc tgctttgcac gttcaataac gaattttaaa gatgtgttta attctggatc
      241 gggttcacct tgtttagcag cagcatagat ttctacaccg aattttgcat aaattttaga
      301 cgttgcaccg tctttagccg tttttttagc aacaatattg gcccatttac gtcccactgg
      361 aatgatctcc tcgaaaattt atttacatta ctggtaaatc gttaacatgt gttaattcgg
      421 catattatat aataacagga aaaatctcgt ctattagaaa taaaaatatt ctaaagtcat
      481 aatcttatat ttaattttat ataaacttat attgttggtg ggatttttta taattgtgtt
      541 ttgaaagcat acttgctttt aatatgtgga aatcattaag acgataataa aactcgtatc
      601 cgcccaagga agcaaacagc ccgcgtttat tgcgggctat ttgccagatg tcttacttaa
      661 tccttcacat ctatttttga taagaaggtt cagaacttat tatcggaacg gcggctcatt
      721 aaaggttcgt aatttacgtg aatgcagtcg gtcgccttct gcgcgcagca aatcgatcgc
      781 ccgaatgcca atttgcaggt gttcggaaat agcaccttca taaaaacggt tagcctggcc
      841 gggaagttta atctcgccat gcaacggttt atctgaaaca cacagtagtg tcccgtatgg
      901 cacgcggaaa cgatatcctt gcgcggcaat ggttgcactt tccatatcaa ttgctacggc
      961 ccggcttaag ttaaaacgaa gtgcagaagc tgagtaacgt aattcccagt tcctgtcatc
     1021 agtggttact acagtgccag tacgtagccg ctgtttaact tcctcaccgg gcctgccgct
     1081 caccagcttg gtggcgtcat aaagcgcacg ttgcacttca gcaatgctcg gaataggaat
     1141 atcgggcggc agaaccgcat caagaacgtg gtcatcgcgt aaataagcgt gtgcaagtac
     1201 ataatcgcca atggcctgac tttcacgtaa tccgccacag tgaccaatca tcaaccagac
     1261 atccgggcgt agtactgcca gatgatcgca gatggtttta gcatttgacg gtcccacgcc
     1321 aatattcacc agagtaatac cctgaccatc ggcggtaatt aaatgccacg cgggcatctg
     1381 gtgttttttc catgcaaggt cggaaatggc ttcttctggc gcttcggttt cggcggtgat
     1441 ccagttcccg ccagcacaag aaagggcaat gtagggacta tcaggatcga ggatctggct
     1501 gcatccccaa cgaacgaatt catccacata acgtgtgtag ttggtaaaca agacgaacgg
     1561 ctgaaaatgt tcaactggcg taccggtata atggcgcaac cgcgcgaggg aaaaatcgac
     1621 gcggcgcgca tcaaaatgcg atagcgggga gaattcagtt gggtgataaa tgccgtctgc
     1681 agtttcatcg ccaatttgcg ccagttctgt tgtagggaaa tagcgagtta acccagcgct
     1741 cattgagcga tcaagtgtca attcagagcc atcgatgaca taaggataag ggatttcatg
     1801 ctgcgagggt tgcaccgaga tatgcgcacc ataatcctga tacagcaacg taagttgttc
     1861 attaagatac gaacgaaaga gagtagggcg agtaatcgtg gtggtgtagc tgcctgcgtg
     1921 agtaaaacga ccaaatgcgc gcgttttggg gggattggtt gtgctaccgt cccaggttac
     1981 ggtcagtgat ggatagacaa aaaaaccttg tttgcgggcg ttttcatcag gtaattcgcc
     2041 acttgtaata tagttgccaa tggcgttgcg taatgcgact acagattgct catacagcgc
     2101 gtcgagttta tccagtgcct gagctggggt cagaccggag cccttattat tcatgtgtgt
     2161 ctcctgttcc atacaattac cgcacatccg atagtatgtc acagaaatgt gaaacaaaag
     2221 tgtaaggcga agatcggatg atcttcgcca ggctatcaag cgcgttgact gtctttcatc
     2281 aacaaagcgg ttattgcaga aatcagacat ccagccagca gatacatggc gacgctatgc
     2341 cagttcccgg caaagtaagt gatgagtgca gcggcaataa aaggtgtaaa tcctccgcca
     2401 accacactgg caacctgata accgactcca gcgccactat agcgataact ggcaccaaac
     2461 atttcggtaa acatcggttg ttgcacacac accaccatgt catgcgcaat gtttgccagc
     2521 attatggaga agaaaactat ccagaaaata gattgtgctt caagcgccat aaagaaagga
     2581 aatgcgctca acgttccgat taacgcacct gtgatataaa ccctgcggcg accaaaacga
     2641 tcggcaagcc aggcaaaaca gggaattgtc aggcagctta atccacctac cagcaaacca
     2701 atattaagga aaagttcgcg cggtaacccc atattctagg ttgaataatt aagtgcaaag
     2761 gcggtaacga tgtacatcgt cagcaactcg cacagccgta gcgcaataat cttcaggaaa
     2821 gcacctggat gtcgtaacag cgcttcgata accgggatgc gttttttcgc ggcagcttga
     2881 ttatgttgct gttgttcgaa ttccgcggac tcctccatgc cattgcgcac ccacaatgct
     2941 cccagtacca gtacgatgct aaacaggaaa ggaatacgcc agccccaact taaaaactgt
     3001 tcgtcagtcg tcatcatact gatcaatgaa accagtccgg ttgaaagcag taaacctaca
     3061 ccgtagccaa cttgtacacc gctactgtaa aaggcttttt tatttttcgg tgcactttca
     3121 acggaaagca acgccgcgcc tccccattcg ccgccgacgg caaatccctg aatggcacgc
     3181 agcgtcacca gtaaaatagg tgcccaccac ccaatggtcg agaatgaagg aagaatacca
     3241 atcaaggctg tcgcgatgcc catcatccag acagtcagca ttaacatgcg cttacgtccc
     3301 agtcggtcgc caaagtgacc gaaaatgaca ccgccgagcg gacggaaaag gaatccgacg
     3361 ccaaaggtag caaatgcggc gagcgttccc atcgccgggc ttacttgcgg gaaaaactcg
     3421 cgattaaaca ccagtgcggc ggtgatgcca tagagtaaaa aatcatacca gtcgacgacg
     3481 gcaccagcga aactgcctaa cgcagctcgt cgggcgcgac ttaacgaaac cgtcctttca
     3541 tcgggacgag tggagatgag cgtggag
//
LOCUS       DAJTNA010000280         3527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTNA010000280 DAJTNA010000000
VERSION     DAJTNA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.286"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..494
                     /locus_tag="OO161_004004"
     CDS             <1..494
                     /locus_tag="OO161_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464857.1"
                     /translation="IAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKYAFGLYWRIA
                     EWPGEGGEGHLSSFFVQSDKASINVAYHHTVNQQKELGTFGAFDHDWHTLAFKFKGSN
                     SINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDTVEVKANKAG
                     AAA"
     gene            634..814
                     /locus_tag="OO161_004005"
                     /pseudo
     CDS             634..814
                     /locus_tag="OO161_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
     gene            738..1085
                     /locus_tag="OO161_004006"
     CDS             738..1085
                     /locus_tag="OO161_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR5464858.1"
                     /translation="MIKGPLRKSAPVSQPEANPKNPLLLNNPFQRENPMSEITSLVTA
                     EAVKEVLRSEEVRSALKQKLRLNLESRLDAEVDAILDELLGVAVVPEPEGIAGDGSAS
                     DGGEPTPDSDMMM"
     gene            1162..1377
                     /locus_tag="OO161_004007"
     CDS             1162..1377
                     /locus_tag="OO161_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5464859.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAAWGE"
     gene            1382..1726
                     /locus_tag="OO161_004008"
     CDS             1382..1726
                     /locus_tag="OO161_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5464860.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1777..2310
                     /locus_tag="OO161_004009"
     CDS             1777..2310
                     /locus_tag="OO161_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5464861.1"
                     /translation="MNTKIKYGLSAAILALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDSKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            2467..2649
                     /locus_tag="OO161_004010"
     CDS             2467..2649
                     /locus_tag="OO161_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464862.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            2798..3265
                     /locus_tag="OO161_004011"
     CDS             2798..3265
                     /locus_tag="OO161_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5464863.1"
                     /translation="MNRMLSAFTVILLVVCGALWLATDHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREATGTSGMDNATGPRLADTAERDYFTLRERLMTMQKQLEGAQEYIRTQCIN"
     gene            3289..3513
                     /locus_tag="OO161_004012"
     CDS             3289..3513
                     /locus_tag="OO161_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464864.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          931 a          759 c          988 g          849 t
ORIGIN      
        1 atattgctgc agacaaggca aaacttttac tggaaaaagg cggggaaatt gctgtccggt
       61 ttaaaatccc cgaaaacgtc aatcttgaga caaccagaaa caagtatgcc tttggtttgt
      121 actggcgaat agcggaatgg ccgggtgagg gtggtgaagg ccatctgagt tctttctttg
      181 tccagtcaga taaagccagt attaatgttg cataccatca tacagttaat caacaaaaag
      241 aacttggcac gtttggcgca ttcgaccatg actggcatac gcttgcattt aaatttaagg
      301 gcagtaacag cattaatgtt actccggtgc ttgatggtgt ggatggacag gcgtttgacc
      361 tggtgaaatg ggccaatact gctaatggac tcaacaggtt tgtcattacg gatattacag
      421 gtagtgcaga aacctaccct gtacttattg atacggtgga agttaaagca aacaaagctg
      481 gagcagccgc ataattgcta aaaaaagccg ccagcggcag gaatggaagc tggcggaggt
      541 aatcccaatg gagaatgtaa agaaaagatg ctttcgtaca ttggtttttt aaatgaaaac
      601 aattctcatt gtcaaccata acggtaagaa attatgacat ttattcatca ggtgatgctg
      661 tacttctgta cggcggtctg tgctgtatct tctttcgggt gggtacaggg ctgtgcgcga
      721 tttctggtac aggcagattg ataaaagggc cgctgagaaa atcagcgcca gtcagtcagc
      781 cggaagcaaa cccgaagaac ccgttactcc ttaacaaccc ctttcagcga gaaaatccca
      841 tgtcagaaat cacatccctg gtcactgcag aggcagtgaa ggaagtcctg cgctctgaag
      901 aagtccggag cgcactgaaa cagaaacttc gcctgaatct tgagtcgcgt cttgatgcag
      961 aagtggatgc cattctggat gagctgctgg gcgtagcagt ggttcctgag ccggaaggta
     1021 tcgcgggtga cgggagtgct tcagatggcg gtgaacctac acctgacagc gacatgatga
     1081 tgtaagcatg cgcaagggac catcggtgtg tgccggtggt ctttatattg ttgtgagctt
     1141 ccggattgcg ggagacgggg tatgtaccag atggaaaaaa tcacaacagg tgtgtcatac
     1201 accacgtcag cggtgggaac gggctactgg ttcctgcagt tgctggacag ggtttccccg
     1261 tctcagtggg cggcaatagg cgtgctgggg agtctgttgt ttgggctgct gacatatctg
     1321 actaacctgt atttcaaaat cagagaggac cgtcgtaagg cggcgtgggg agagtaaagc
     1381 gatgaagaaa aaatacgaac tggttgttaa agggataaat aattacccga ataagattac
     1441 tgttactgtg gcactggaaa ttggtgggta tccgtcactg ttgttgccag atgtggcgat
     1501 tagtcttgac cgtactgaag atgccacgct ggagttttac gaagctgagg cgaaaaagca
     1561 ggcgaagcag tttttcatgg atgttgctgc cgggttatgt gaaggggatg gtccgttgcc
     1621 ggaaaagcgc cccgtaattt tagaggcgca ggatgtgttg ataacctaca gaggaaaact
     1681 accgggaata attactggtt ctctgaagac tccaccgctg gcctgaagac ttaacatatc
     1741 cagggatttg aaatcgataa accctgataa atatccatga acaccaaaat caaatacggc
     1801 ctgtcggctg ccattctggc gctgattgcc gcaggtgcgc ctgcgcctga aatcctcgac
     1861 cagtttctgg atgaaaagga aggtaaccac accacggcat accgtgatgg cgcgggtatc
     1921 tggaccatct gccgcggtgc catcctggtg gatagtaaac ctgtcgtccc gggcatgaag
     1981 ttgtcgaagg aaaaatgcga ccaggtcaac gccattgagc gtgataaggc gctggcatgg
     2041 gtggagcgca atattaaagt gccgctgacc gaaccccaga aagcgggtat cgcgtcattt
     2101 tgtccctata acattggccc cggtaagtgt ttcccgtcga cgttttacag acggattaat
     2161 gctggtgacc gcaggggagc atgcgaagcg attcgctggt ggattaagga cggtggcaga
     2221 gactgccgta tccgctcaaa taattgctac ggtcaggtat cccggcgaga ccaggaaagt
     2281 gcgctggcgt gctgggacat cgacagataa gcagaatatt ttgctgaaaa ataaggcatg
     2341 agcacgcgga cggataacac gaaatcctgc gaactggcga aacgtaagtg aataaaagta
     2401 aaaaccccgt ttgttggcac caagcggggt tttgtgtttc tgaccttgag taaggcaagg
     2461 gagaacatgg cgaagtataa acgaattctg ttgaggttga ccatgaaaaa cggccttgaa
     2521 ctgaaagcgc ctgtaactga tgacatcagc agagcactgg cttttgccat taagtgggtg
     2581 gcggtcggtg ttgctgtgtc cccgatgctg tatgggctgg caaaactggt cattgcgttg
     2641 aaatcgtgaa gggaggatta agcatgtcag acaaactcat aacgctggcg aagatcctct
     2701 gtgtaattgt cggcatttca ttttcactaa tgctggttgc tctttttctt tccatggcct
     2761 ggatgatgtt gtcttcgtcg gggctgctgg ggtgaatata aaccgaatgc tttccgcgtt
     2821 taccgttatt ctgctggtgg tctgtggtgc gctgtggctg gcaacagacc attaccgtga
     2881 taacgccatc acctacaaag cgcagcgcga taaaaaagcc agagagctgg agctggcaaa
     2941 cgcaaccatt actgacatgc agcagcgcca gcgtgatgtt gctgcgcttg atgccagata
     3001 ctcaagggaa ttagccgatg cgagagctga aaatgaaact ctgcgtgctg atgttgccgc
     3061 tggtcgtaag cgcctgcgga tcaacgccac ctgctccggt accgtgcgtg aagccaccgg
     3121 cacctccgga atggataatg caaccggccc ccgactggca gacaccgctg aacgggatta
     3181 tttcaccctc agagagcggt tgatgacaat gcagaagcaa ctggaagggg cgcaggaata
     3241 tatccgcact cagtgtatta actagtattt ttgttatccg gagaatgcat gaagaaatta
     3301 ctggtaaccg taaagccttt tcagggaaca attccgttcc gtattttgca gcgtggtcgt
     3361 gttcttgttg aaggttcgtt cagtggtaaa tgtacgcaat tacactcccg gacctttcag
     3421 gtgaatgcca cgaatgaaga gctaaccgtt gagtgtacga tgaatgccgc taaatgccgc
     3481 atggtatccg ctgcattaca gccagtgtgt tgagcgacct tattaac
//
LOCUS       DAJTNA010000281         3447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTNA010000281 DAJTNA010000000
VERSION     DAJTNA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.287"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            102..221
                     /locus_tag="OO161_004013"
                     /pseudo
     CDS             102..221
                     /locus_tag="OO161_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            471..1337
                     /gene="ospB"
                     /locus_tag="OO161_004014"
     CDS             471..1337
                     /gene="ospB"
                     /locus_tag="OO161_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5464865.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKSVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1665..2405
                     /gene="phoN2"
                     /locus_tag="OO161_004015"
     CDS             1665..2405
                     /gene="phoN2"
                     /locus_tag="OO161_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5464866.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(2765..2851)
                     /locus_tag="OO161_004016"
     CDS             complement(2765..2851)
                     /locus_tag="OO161_004016"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464867.1"
                     /translation="MLDNAMKSKDSKMIDFFIKKWSGIRQTI"
     gene            2994..3447
                     /locus_tag="OO161_004017"
                     /pseudo
     CDS             2994..3447
                     /locus_tag="OO161_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1068 a          676 c          706 g          997 t
ORIGIN      
        1 agactggccc cctgaatctc cagacaacca gtatcactta tttaagtgat actggttgtc
       61 tggagattca gggggccagt ctagcagcgg taagagaagt tcatcatact tggcgcagtt
      121 acgcgatgtt tctgctagag gtgatgccgt aaagaacggc agaacattac cggaacaaga
      181 tagcggtcta cctgcactgg tatcagaccc ggggcttccc cggatgatct ccacggtact
      241 ttaggtactg tgtctatatt atgtaggtag gatcaattaa ctttgtagtt gaaattcttt
      301 cattgaaata atgaataaaa aagacaaacc gcattaaaca atgccagaat ataattgcat
      361 ttttgttccg tttttttaaa tcatagtata caaagaagct tgcatactat atgaaaatat
      421 aacacccttt actatgatgt ttataaaaca atatatggag tcatgtaggt atgaatttag
      481 atggtgttag accatactgt agaatagtca ataaaaagaa tgaaagcata tcagatattg
      541 catttgcaca tataataaaa agcgtaaaaa attcatcatg tactcaccca aaagcagcat
      601 tggttttttt aggagagaaa ggtttttgtg atagcaatga tgttctatct attatgggac
      661 aacaaatacc aagagtattt aagaacaaga tgttatatga ttatgttttt aaaaatgaaa
      721 aaagtaaaaa tgattttcta aaaatggctg aatcatggct accacagagt gaaccaatag
      781 taataaataa tgatgatgac gcattgaatg ctgctgctta tttttctgta aaaaaagcga
      841 aaataaaaac agtaaacgac actgatttta aagagtataa taaggtttat attcttgggc
      901 acggtagtcc tggttctcat caattaggcc ttggttcgga acttattgat gtacaaacaa
      961 tcatttcaag aatgaaagac tgtggtattc taaatgtgaa agatatccgt tttacttcat
     1021 gcggctccgc tgataaagtg gctcctaaaa attttaacaa tgcccctgct gaaagtcttt
     1081 cttgtatcct taactctctg ccttttttta aggaaaaaga atctttgcta gagcagataa
     1141 aaaaacacct tgaaaacgat gagtcattga gtgatggtct aaaaatatcc ggctatcatg
     1201 gatatggagt tcactatggt caagagcttt ttccctactc acattatcgt tcaacttcaa
     1261 ttcctgctga tccggagcat acagtaaaaa gaagctctca gaaaaagact tttattatca
     1321 ataaagaact ggattagtat aaaaatttta acctatagtt aattgtttgc atttaagatg
     1381 aagttatttt ttctttagta gaaaggtgct ccctatgagg gaaaatcaca aatcatcata
     1441 atacagagac aaaacgatac gaacaaatag ataaaaaaca tcattctttt accacattat
     1501 ttttcgtaaa tatgaaaaat aatgttatta ctttaatata agactatttt ttgtttttcc
     1561 atcactctgt tcaaattttt ccgcatgact tgtgtttttt gtaacacagc tcgtttttta
     1621 cagctgacca aaatcatcaa ttaattatgc taaggaaata aattatgaaa accaaaaact
     1681 ttcttctttt ttgtattgct acaaatatga tttttatccc ctcagcaaat gctctgaagg
     1741 cagaaggttt tctcactcaa caaacttcac cagacagttt gtcaatactt ccgccgcctc
     1801 cggcagagga ttcagtagta tttcaggctg acaaagctca ttatgaattc ggccgctcgc
     1861 tccgggatgc taatcgtgta cgtctcgcta gcgaagatgc atactacgag aattttggtc
     1921 ttgcattttc agatgcttat ggcatggata tttcaaggga aaatacccca atcttatatc
     1981 agttgttaac acaagtacta caggatagcc atgattacgc cgtgcgtaac gccaaagaat
     2041 attataaaag agttcgtcca ttcgttattt ataaagacgc aacctgtaca cctgataaag
     2101 atgagaaaat ggctatcact ggctcttatc cctctggtca tgcatccttt ggttgggcag
     2161 tagcactgat acttgcggag attaatcctc aacgtaaagc ggaaatactt cgacgtggat
     2221 atgagtttgg agaaagtcgg gtcatctgcg gtgcgcattg gcaaagcgat gtagaggctg
     2281 ggcgtttaat gggagcatcg gttgttgcag tacttcataa tacacctgaa tttaccaaaa
     2341 gccttagcga agccaaaaaa gagtttgaag aattaaatac tcctaccaat gaactgaccc
     2401 cataaagctg gacagcctgt atcaggctat ggagggccca tagacaaatc taccctatat
     2461 gagcatagga ggagtctatg ggcacaccac gttttacccc tgaatttaag ggattactgg
     2521 aaaggctggg acatatccct ccggcagaag cagaaaaagc ttattatgct gccatcggaa
     2581 acgatgatct ggcaacctga gttcacagat aaaacgttct ctaggaaact cggggcggtt
     2641 ccgttcacca catgcaatgt ggtgttgcag gggaacggtc tgcaccatcc cctatgtcga
     2701 tcaatataac agaaatgaca acttcagatt cagggcacaa cctaaatata ttttaggtca
     2761 cctctcaaat cgtttgcctg ataccgctcc attttttaat aaaaaaatca atcatttttg
     2821 aatctttgct tttcattgca ttgtcaagca tagtgtccgt aagcgtacag cctgaaccgt
     2881 ctggtcagaa tctgacgaat tagacaaagt ggtgtccacc aaataagtag tgggaaccgg
     2941 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
     3001 ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
     3061 gcactgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
     3121 tcacttacac cgcttctcaa cccggtacgc accagaaaat tattgatatg gccatgaatg
     3181 gcgttggatg ccgggcaact gcacgcatta tgggcgttag cctcaacacg attttacgtc
     3241 acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat
     3301 cgtctgcgcg gaaatggacg aacagtgggg atacgtcggg gctaaatcgc gccagcgctg
     3361 gctgttttac gcgtatgaca ggctccggaa gacggttgtt gcgcacgtat tcggtgaacg
     3421 cactatggcg acgctggggc gtcttat
//
LOCUS       DAJTNA010000282         3432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTNA010000282 DAJTNA010000000
VERSION     DAJTNA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.288"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            139..1200
                     /locus_tag="OO161_004018"
     CDS             139..1200
                     /locus_tag="OO161_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR5464868.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARLRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKMFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(1313..2812)
                     /gene="ansP"
                     /locus_tag="OO161_004019"
     CDS             complement(1313..2812)
                     /gene="ansP"
                     /locus_tag="OO161_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR5464869.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            3079..>3432
                     /locus_tag="OO161_004020"
     CDS             3079..>3432
                     /locus_tag="OO161_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5464870.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWA"
BASE COUNT          953 a          881 c          829 g          769 t
ORIGIN      
        1 aaatcagtaa gttggcagca ttacccattt gagaaaataa tgattaccat tcccatttat
       61 aacaagagcg tgacgatgat tacgcttagc gaagcattgt gaagcagcaa aaatattggt
      121 tcatcaaagg gagtcgtcat gcatttacgt catctgtttt catcgcgcct gcgtggttca
      181 ttactgttag gttcattgct tgttgcttca tcattcagta cgcaggccgc agaagaaatg
      241 ctgcgtaaag cggtaggtaa aggtgcctac gaaatggctt atagccagca agaaaacgcg
      301 ctgtggctcg ccacttcgca aagccgcaaa ctggataaag gtggcgtggt ttatcgtctt
      361 gatccggtca ctctggaagt gacgcaggcg atccataacg atctcaagcc gtttggtgcc
      421 accatcaata acacgactca gacgttgtgg tttggtaaca ccgtaaacag cgcggtcacg
      481 gcgatagatg ccaaaacggg cgaagtgaaa ggccgtctgg tgctggatga tcgtaagcgc
      541 acggaagagg tgcgcccgct gcaaccgcgt gagctggtag ctgatgatgc cacgaacacc
      601 gtttacatca gtggtattgg taaagagagc gtgatttggg tcgttgatgg cgagaatatc
      661 aaactgaaaa ccgccatcca gaacaccggt aaaatgagta ccggtctggc gcttgatagc
      721 aaaggcaaac gtctttacac cactaacgct gacggcgaat tgattaccat cgacaccgcc
      781 gacaataaaa tcctcagccg taaaaagctg ctggatgacg gcaaagagca cttctttatc
      841 aacatcagcc ttgataccgc caggctgcgt gcatttatca ccgattctaa agcggcagaa
      901 gtgttagtgg tcgatacccg taatggcaat attctggcga aggttgcggc accggaatca
      961 ctggctgtgc tgtttaaccc cgcgcgtaat gaagcctacg taacgcatcg tcaggcaggt
     1021 aaagtcagtg tgattgacgc gaaaagctat aaagtggtga aaatgttcga tacgccgact
     1081 catccaaaca gcctggcgct gtctgccgat ggcaaaacgc tgtatgtcag tgtgaaacaa
     1141 aaatccacta aacagcagga agctacccag ccagacgatg tgattcgtat tgcgctgtaa
     1201 tagatatggc gagggtgaaa acggtgagac ttgcctgcct tttatcacgg cgtatccggc
     1261 actttcgtca gtaatctgaa tttgcccggc acgtagccgg gcaattttgc aattaactcg
     1321 ccgtttcagg cttaaacaca atttcctgtt tttcttcatc ttcctcgacg actggcgcag
     1381 tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca gcctataacc agcagaatac
     1441 cgataatcgg cagcgccgcg atagtataag tcccgttcgg gtaatcgaac gccatcagca
     1501 caaggacact cagtaaaaac agtaatgtca gccaggaagt gaagggcgcg ccaggcagtt
     1561 taaaactgac atccgctgct ttgccttctt taatcgcttt acgcaggcgc atctggcaca
     1621 cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc gaagttcaac acaatctcaa
     1681 atacgcgcga cggcaccaga tagttgagga atacgccgac gacatacaca accagtgtcg
     1741 ctagaatccc ggcatacggc acatgctgac gactcatttt cgccataaaa ctcggtgcgg
     1801 aaccgcccat cgccattgag cgcagaatac gtccggtgca gtacagacct gagttcaggc
     1861 tggagagggc agcggtcagc accacaatgt tcataatgct gccgatatat ggcacaccca
     1921 gtttagagaa aaacgtcacg aacggacttt gccccgcctg atacgcgctc cacggcaata
     1981 acataaccag caacaccacg gagccgacgt aaaacaggcc aatacgccaa atcacactgt
     2041 taatggcttt aggcaccatg gtctgcggat ctttacattc tccggcagct gtacccacca
     2101 tttcaatgga ggcaaaagca aacactacgc cctgaatcaa caccagcgca ggcagcaaac
     2161 cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa gccagtggtg ttgccatcca
     2221 gcggctgacc actaccgagg aacactgtac ccacgaccaa aaaggtcaca atggcgagca
     2281 ctttaataag cgcaaaccag aactccatct ccgcaaacca tttcacaccg atcatattca
     2341 tggtgccaac gatggtaagt gcagcgagcg caaagaccca ctgcggcacg ccgccaaacg
     2401 caccccagta atgcatatac agagcgacgg cggtgatatc aacaatcccc gtcatcgccc
     2461 agttgatgaa gtacatccag ccagcaacat aagcggcttt ctcacccaaa aactcacggg
     2521 cataagagac aaaactgcca ctggaagggc ggtgtagcac cagctcaccc aatgcacgca
     2581 gaataaaaaa cgaaaacaag ccacaaatta aataaaccag tgccagtgct ggacccgcca
     2641 tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc aatcgcgccg ccaatggcga
     2701 tcatctgtac ctggcgattg cccatcgctt tgtgataccc ctcttcgtgg gcattaagcc
     2761 agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc gtgtttactc attgctctcc
     2821 ctgattgctt taatgaaaaa gtcatataag ttgccatgaa caatatttat tcatcctggc
     2881 gatgatagtt atcaccatta ttctttatgg gtataaaact atcggcgcag catcctaccc
     2941 gttatcgata tacgatgcaa aacatcccct tacaatcctg aaggggattc atacaactga
     3001 cgaaaaaatg acaaatactt ttgctggtta acctgtgtac tgtcctacac ttaatcttta
     3061 aaagattgtg aggggcatat gattaaggtg tatggcgtac ccggatgggg ctctacaatc
     3121 agtgagctga tgctaacact ggctgatatt ccttatcagt tcgttgatgt cagtggtttt
     3181 gaccacgagg gaacttcacg cgagttattg aaaaccctga acccgttgtg tcagattccg
     3241 acactggcgc tggaaaatga cgagattatg acggagacag cggcgattgc attgatggtt
     3301 ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg ccgaacgtca gcagtttcaa
     3361 cggctattgg tctggctggt tgctaatgtc tatccaacat tcactttcgc cgattaccct
     3421 gagcgttggg ct
//
LOCUS       DAJTNA010000283         3420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTNA010000283 DAJTNA010000000
VERSION     DAJTNA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.289"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..2369)
                     /locus_tag="OO161_004021"
                     /pseudo
     CDS             complement(1..2369)
                     /locus_tag="OO161_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            complement(2430..3032)
                     /locus_tag="OO161_004022"
                     /pseudo
     CDS             complement(2430..3032)
                     /locus_tag="OO161_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(2969..>3420)
                     /locus_tag="OO161_004023"
     CDS             complement(2969..>3420)
                     /locus_tag="OO161_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5464871.1"
                     /translation="AIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRG
                     DDWWRHGQNLYLDNLEATGLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHH
                     IPEQLSKRERYTDKWQRRTHSLWRHREWHASAFTGICNDLAAASTFV"
BASE COUNT          698 a         1111 c          876 g          735 t
ORIGIN      
        1 gcgaacgtaa aaataataat catggcccgc ctgatattga tactggcggc tatccagtac
       61 agcgccgtgc caagatagcg ggcgctggtt tcaacctgcc tgatatccgc aatccgcttt
      121 tccgaaaacc agaactcaaa ctgcaccgtc gggtcatata tagccagttt cgggaccgcc
      181 gttatctgaa aataccccgg tatcagttca atagtgacag gcgctgccgg tgccgcaatc
      241 cggaacgata ccgacgccgg atcgccctgc tgcccccggg catttaccgc ccggactgtc
      301 agactgtaat tccccagcgc cagctgcctg aagcggtatg tggtttccgt cgtcctggct
      361 gtgctgacca gccgctcact gccgtcgtcc gctgccacgg tcaggcgaag caggaagctc
      421 acacccttca ccaccttcgg cgtgtcccat cgcgccagca cctgatattc cccgctgtct
      481 gcggtgactt cggcggtcag atgctgcacc gctggcggcg tgacaccatt caccgtgccg
      541 cgccggtcac cgtcaaagtg tgccccgtta tccacgatgg cctctttttc cggcacatgc
      601 tgcacggcgg tgatggcata cgtgccgtcg tcgttctcac ggatactcac gcagcggaac
      661 aggcgctggc gcagcgtcgg cagcttcagc ccccacacgc tgtattctgc aacgccgtca
      721 ggaacccggc tcactttcac cttcacgccg tcggtgacgg actgaacctc cacgctgacc
      781 ggattgccat ttccgtcaac caggcttatc agcgtggtac cggaggacgg cagcgtgatt
      841 tcacggtcga gcgtcagcgt ccgggtctgg ctgttcaccg ccagtacgcg accaccggtg
      901 ctgataccgg cataatcatc atcgcagatt tcaatgacat cacccggtac atggcgaagc
      961 ccttctgcgc cgacgctgaa gtccacggtc tgcgtttcca gcagttccgt tttaatcagc
     1021 cacagcccgg cgcggtgcgc ctgtccccgg ctggtacagc caaaggcatc catcttcgtg
     1081 gcgttacgac cgtaacggag aatggcctgc gtgtcctcca caagctctgt cgccgtctca
     1141 tgaccgttgt ccggatcaat ccagttcacc tcaacgacat tatggcggtc cttcagggcg
     1201 ctgaagctgt agcggaacgg cgcgccatca tccggcatca ccacattact gcggttatag
     1261 gtccacactt tatccgacgg tcggtcctgc acgaacgtca gcgtctgccc gttccatacc
     1321 ggcatacagc gcatcgccga gcagaaatca ctgagaacat cccacgcctt acgctgtgtg
     1381 gtcaggtaag cgttacaggt gatgcgcggc tccgtgccgc caaatccgtc cggcactgac
     1441 tggtcgcagt actggccgat gacatacagc gcccatttgt ccacatctgc cgcaccaaga
     1501 cgtttcccca tgccgtagcg cggatgagtg agcatgtccc acagacacca ggccatgttg
     1561 ttgctgtatg ctggcttaag cgttccgtcc cagataccgc tgtattgccg cgtctgcggg
     1621 ttatagttcg acggcacctg cagaatgcgc ccgcgaagat gataattgcg actcacctgc
     1681 tggttaccga actgctctga atccacctgc acgccgacca gcgccgtgtt cgggtagaac
     1741 tgtttcacat cgatgatttc ggtgtacgac gaccagagcg ttttgttctg cagctggtct
     1801 gtggtgctgt ccggtgtcat cctgcgcatc cggatattaa acgggcgcgg cggcaggtta
     1861 cccatcacca ccgaggccag atactgcgaa gtggttttgc ccttaatggt gatgtctttt
     1921 tccgtcaccc agccaccgtt acgctgtatc tgaaccagca ggcggacttc cgacggattc
     1981 cggtccccct ttgaggtgtt ttccaccagt gcctgcacac cgaaggtcag gcgcagacga
     2041 tcaatgtttg ccgacgtgat ggtccgggtg atcggcgtgt cgtatttcac ttccgtaccc
     2101 agcacagtct ctgagccgga agattcaaac ccctccggcg gtgtctgctc ctgctcgccg
     2161 gcgcggaaca ccaccgtaac accggaaaag tttgtcttcc cctcgctgtc caggaccggc
     2221 gtaccgttca gcagcacgct ttttaatcca tccaccggac cgtcaaccgg cccttcgctg
     2281 atggcatcaa tcacactcag caactgcgtg gatttcaggt tgtccttcgc ttcgcgcggg
     2341 gtatgcccct tactgctgcc tttacccatt cctcacgctc cataaacgac aaaaccgccc
     2401 gcaggcggtt tcacatgaaa cattttgcat cagcgaccaa tcaccacaac ctgaccacca
     2461 tccccttcgt ctgccgtgct gatctcctga gataccaccc gcgaccccac gcgcatttca
     2521 ccgtacagaa caggcagaac attgccctag gcaaccatgt tatccagtga ggagaaacag
     2581 gtgttctgtt tgccgttatc cgttgtctgt gtacggggag ttctggcttt cggtgccagc
     2641 atctgtgcaa caccgccaag cgtcatactg gcaccgagag aaaacagcag attactcgcc
     2701 ataattccta cccccggcat ccatatagca accgccataa cagctgcccc cagcaccgcc
     2761 tgaaacacac cgccactttt agctcccacc agacgcggta ctatgtggat cactgcacca
     2821 tttgccagcg gctcattaag acgggcagac aattcggttt cacctgcatc acgcccggca
     2881 atgcgcacct gataccagcc gtcactcagt ttctgacgaa acgccgggag ctgtgtggcc
     2941 agtgcgcgga tggcttcagc ccccgttttc acacgaaggt cgatgcggcg gccaaatcgt
     3001 tgcaaatccc cgtaaaggca gatgcgtgcc attcccggtg acgccagagg gagtgtgtgc
     3061 gtcgctgcca tttgtcggta tacctctctc gtttactcag ttgttcagga atatggtgca
     3121 gcagctcacc gtcgccgcag taaatggcgg catgattcgg caccgatgaa ccaaaacagc
     3181 acagcagcac atcgcccggt tgtgctgatg acaacggcac ctgatacagc cctgtggcct
     3241 ccagattatc cagatagaga ttctgaccgt gacgccacca gtcatccccg cgatgaaaat
     3301 ccggcatctc aatccccgcc agatgataag catcccggaa cagcgtgtaa cagtccgtca
     3361 ccccgtgctc aaagcgccgc ccggtgagat gcggcacaca gcggaacttg tgaatcgccc
//
LOCUS       DAJTNA010000284         3390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTNA010000284 DAJTNA010000000
VERSION     DAJTNA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.290"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..734
                     /locus_tag="OO161_004024"
     CDS             <1..734
                     /locus_tag="OO161_004024"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5464872.1"
                     /translation="EIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTIVYNVN
                     DDTLDGNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGK
                     DLRASRAAAENIIPHTTGAAKAIGLEIPELSGKLKGHAQRVPVKTGSVTELVSILGKK
                     VTAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGDLQLVK
                     TVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(776..2215)
                     /gene="aldA"
                     /locus_tag="OO161_004025"
     CDS             complement(776..2215)
                     /gene="aldA"
                     /locus_tag="OO161_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5464873.1"
                     /translation="MSVPVQHPMYIDGQFVTWLGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2412..3212)
                     /locus_tag="OO161_004026"
     CDS             complement(2412..3212)
                     /locus_tag="OO161_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5464874.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
BASE COUNT          868 a          878 c          803 g          841 t
ORIGIN      
        1 cagaaatcat tgtcgaatgt actggctttt atacctccgc cgagaaatcg caggcgcatc
       61 ttgatgctgg tgcgaagaag gtgttgattt ccgcccctgc cggtgaaatg aaaactatcg
      121 tttataacgt caatgacgac actctggatg gcaacgacac cattgtttcc gtggcgtcat
      181 gcaccactaa ctgtcttgcg ccgatggcca aagccttgca tgacagtttc gggatagaag
      241 tcggcacgat gacgaccatt catgcctata ctggcaccca gtcactggtg gatggcccac
      301 gtggtaaaga tttacgtgct tcacgcgcag cggcagaaaa tatcattccc cacactacgg
      361 gggcggcaaa agccattggt ctggagatcc cggaactgag cggcaaactg aaaggtcatg
      421 cgcaacgcgt gccggtgaaa acaggttcgg tcactgaact ggtatcgatt ctcggaaaaa
      481 aagtgactgc cgaagaggtg aataacgcac ttaaacaagc aaccaccaat aacgagtcat
      541 ttggttatac cgatgaagaa atagtctctt ccgatatcat tggcagccat ttcggttcgg
      601 tgtttgatgc cacgcaaacg gaaattaccg ccgtgggcga tttacaactg gtgaaaacgg
      661 tcgcctggta cgataacgaa tatggcttcg tcacgcagct tattcgcacc ctcgaaaaat
      721 tcgctaaact ctgacgcgca caggcggagg aaaaaacctc cgcctctttt actcattacg
      781 actgtaaata aaccacctgg gtctgcagat attcatgcag gccatgttta ccatctgcgc
      841 cgccaatacc ggatttacgc catccggcgt ggaagccttg catagcttcg aagttttcac
      901 ggttgatgta agtttcacca aacttcagcc ctttaatggc tttcatcgcg acgttcagat
      961 tttgggtata gattgatgag gtcaggccgt aatcactgtc attagccatt gagatagcct
     1021 cttccagcgt gtcaaatgcg accaccggca gaaccgggcc aaaggtttcc tcatgcataa
     1081 tcgacatttc ctggcgaaca tccagcagca atgtcggcgg ataataatat cctttcccct
     1141 ctaccgcttt gccacccaac gccactctcg ccccttcttc tactgcgcgc gccacttttt
     1201 gctcaaccct ttccaacgcc gcggcgttaa tcaacggccc catcgcaatg tcgttgcgtt
     1261 cagcggggtt accaaattga accgcctgca tcgcttcacc cagccgattg acgaactgat
     1321 cataaatgcc tttctgtaca taaacacgtt ctgcacagtt acacacttgc ccactattaa
     1381 tgacgcgtga atcaacgatg gctttgactg ccagttcaag atcggcatcg tccattacga
     1441 tagctggtgc tttacccccc aattccagac acactttggt gatgtttttc gccgcagtcg
     1501 ccatgatctt ctcacctgca gagacgctgc ctgtcatact gaccattgcg acctttgggt
     1561 tacccgccag ttcttgccca acggtttcac cacgccccag tacaaggtta aacacgccgc
     1621 gcggaaggcc tatttcatcg acgattttgg cgaatgcaat cgcattgttt ggcgtaaatt
     1681 cactaggttt aatgacgatg gtattaccgg tcaaaagagc gggagccatt ttgcgggcaa
     1741 tgaggaagaa cgggaagttc cacggcagaa tgccggtagt cacgccaagc gcacgtttaa
     1801 acaaaagaat attttctcct ggacgatcgc tttgaataat ctcgccctcg taacgccgtg
     1861 cccactccgc catgtaatcg atatagtcgg cagtaaaagc cacttcgact tcagccagct
     1921 gctggatctt gcccccttct tcaacaatca gcgcactgat ttcactggcg cgttcgcgga
     1981 tcccggcgga gattttgcgc aaccaactgg cgcgttcaat agcaggcaac gcttcccatt
     2041 ctggttgtgc acgttctgct gcatcgattg ccttacgggc atcctcggcc tgaccatcgg
     2101 gtatgcggga aatgacagcc tctgtagcag ggtttaccac atcaatccat gcgtctccaa
     2161 gccaggtaac aaactgtcca tcgatataca taggatgttg aacgggtact gacatgggcg
     2221 actcctgtga tttatatgtt tgttcttggt aaatacattg ttaattaaca tcaatcatca
     2281 tagagacggc atcgtgaaag gacaattctg tgaagggctt cataaaaaca agtgtaaaag
     2341 gaataaaagg cagaaataaa agtcaggagt catgcccgtt tgcaaacaag tggttcacaa
     2401 acgggcaatt tttaacgtaa cgagcgactt tccatcgcct cgatgagcac cgcatcgtgt
     2461 ttaagcgttt gccatgcatg gatgacttct gccggaaaat caacgtgaac gataaaatct
     2521 cgcccgcggg gaccgtagcc atcgctatca tcgcgtaaac gcggcagctc gccagtgagt
     2581 agtgagagat aacgctcaac cggccataat ccttgtaact gattaataaa gattacactg
     2641 tctgcgttat ttcctaactg aggaacgaat ccgggataac ttaaccagcg tggtgcatcg
     2701 ggattgtccc cagtcatacg gcggaacgtc gccatcgtgc gccgctgcat ggtggggtcc
     2761 tgaacaacga tagccgtatg aactcgttct acggcctgat taaatagcgc gatgctaaag
     2821 cgtgcgtttt caccgcagtt tgttgactgg tcttcaatcc agattttttc atgcggaatg
     2881 tgccagaact gatgagcgat atccgccagg atggtcgctt ctgctctgcc agtggtgcgg
     2941 atagtgttgt agtgcggatg ctgtgcgatg gcgctataca aaaaagttgt cgagtgaccg
     3001 ataccaccac taatcagtaa aggaatttgc tgatcgcgag caatcttaca tgccgcgtcg
     3061 atagtcggca taaccgcatt gcctgcaagg atcacgcaat cggcctgata cggcacctca
     3121 ccggagaaat catcctgcgc cagccactgt ccgataacat ttatggcatc tatagttgcc
     3181 ggcgaaagcg tcggaaacgg ggtaatgttc atggttcctc cttatcgttc ccttaagaat
     3241 actgcgtgag ataatagtga gaagctgagc actctgaaag gaggaagtcg tatgtcctta
     3301 accgcactcc gcaagcctga catatctttt aaggaaatgc tgtagataaa cattattcat
     3361 cttgcagcag agaattgtat ttttctgaat
//
LOCUS       DAJTNA010000285         3258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTNA010000285 DAJTNA010000000
VERSION     DAJTNA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.291"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            97..3258
                     /locus_tag="OO161_004027"
                     /pseudo
     CDS             97..3258
                     /locus_tag="OO161_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723107.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
BASE COUNT          943 a          825 c          837 g          653 t
ORIGIN      
        1 agcggggttg caatcgcctc cctgaccggc acgttagcgg gcacgcatat gatcacggct
       61 cgtctggcta acagcaatgt cagcgatgca cagccaatga cgtttgtggc ggataaagac
      121 agagcggttg tcgttctaca aacatcgaaa gcggaaatca ttgggaatgg cgtggatgag
      181 acgaccctga cagcaacagt gaaagatcct tttgataacg tggttaaaaa tctgtcagta
      241 gtcttccgca cctcccccgc agacacgcaa ctgagtctga acgcgcgtaa tactaatgag
      301 aacggtattg ctgaagttac ccttaagggc acggttttgg gtgttcatac agccgaagcc
      361 atactgctta acggcaacag agatacgaag atagtcaata ttgcgcccga tgccagcaac
      421 gcgcaggtca ccctgaacat ccctgcacaa caggtggtga cgaataacag cgacagcgtg
      481 cagctgacgg cgacggtgaa agatccgtcg aatcatccgg tggcgggaat aacggtgaac
      541 ttcaccatac cacaggacgt ggcggcaaac tttacccttg aaaataacgg tattgccatc
      601 actcaggcca atggcgaagc gcatgtcacc ctcaaaggca aaaaagcggg cacgcatacg
      661 gttaccgcaa cgctgggtaa caataatgcc agcgatgcgc aaccagtcac cttcgtggcg
      721 gataaggaca gcgcggttgt cgttctgcaa acatcgaaag cggaaatcat tgggaatggc
      781 gtggatgaga cgactctgac ggcaacagtg aaagatcctt ttgataacgc agtaaaagat
      841 ctacaggtca ccttcagtac caaccccgca gatactcaac ttagtcagag caaaagcaat
      901 actaacgaca gtggtgtggc cgaagttacc tttaagggca cggttttggg tgttcataca
      961 gccgaagcca cactgcctaa cggcaacaac gatacgaaga tagtcaatat tgcgcccgat
     1021 gccagcaacg cgcaggttac gctgaacatc cctgctcaac aggtggtgac gaataacagc
     1081 gacagcgtgc agctgacggc gacggtgaaa gatccgtcga atcatccggt ggcgggaata
     1141 acggtgaatt tcaccatgcc acaggacgtg gcggcaaact ttaccctcga aaataacggt
     1201 attgccatca cccaggccaa tggggaagcg catgtcacgc tcaaaggtaa aaaagcgggt
     1261 acgcatacgg ttaccgcaac gctgagtaat aacaatacca gtgattcaca gccggtaacg
     1321 tttgtggcgg acaaaacctc ggctctggtt gttcttcaga tatcaaaaaa tgagatcaca
     1381 ggtaatggcg tagatagcgc aacgctaact gcaacggtca aagatcagtt cgacaatgag
     1441 gtgaacaatc ttccggtaac attcagcaca gcttcttcag gcctcaccct gaccccaggg
     1501 gaaagtaata ccaatgagtc tggcatcgcg caggccactc tcgcaggcgt tgcctttggt
     1561 gagcagacgg ttactgcatc actggctaat actggtgcca gcgacaacaa aactgtgcac
     1621 tttattggcg acacaacggc ggcaaaaatt atcgagttga cgcctgaccc aggcagcata
     1681 atcgccggta ccccacagaa cagcaccggt agcgtaatca ccgctacagt cattgataat
     1741 aatggctttc cggtgaaagg tgtgactgtg aacttcacca gcaacgcagc gacagccgaa
     1801 atgacgaatg gcggccaagc cgtaacgaac gaacagggta aggctaccgt cacttatacc
     1861 aatacccgct cctcgataga atcaggagcg agaccggata ccgttgaggc cagtctggga
     1921 aatggtagct ctacgcttag cacatcaatt aatgtcaacg ctgatgcgtc tacggcacat
     1981 ctcaccttgt tacaggcact tcttgataca gtctccgcag gcgacactac caatctgtat
     2041 attgaggtga aggataatta cggcaacggt gtaccccagc aggaggtaac cctcagcgtt
     2101 tcaccaagtg aaggcgtgac tcccagtaat aacgctatat atacgaccaa tcacgacggc
     2161 aatttttacg caagctttac cgctacaaaa gccggggttt atcaagtgac ggcaaccctc
     2221 gaaaatggcg attcgataca acaaacggtg acctatgtgc cgaacgtcgc gaatgccgaa
     2281 atcacgctgg cagcctcgaa ggatccggtg attgccgaca ataacgatct cacgacacta
     2341 acagcaacag tcgctgatac agagggcaat gcgatagcca acactgaagt aacatttact
     2401 ctgccggaag atgtgaaggc gaacttcacg ctgagcgatg gcggtaaagc ggttactgat
     2461 actgaaggca aagcgaaagt cacgctgaaa ggtacaaaag caggcgctca tactgttaca
     2521 gcatcgatgg ctggcggtaa gagtgagcag ttggtggtga attttattgc ggatacgctc
     2581 acagcgcagg ttaatcttaa cgttaccgag gacaacttta tcgctaataa cgtcgggatg
     2641 accaggctgc aggcaacagt gactgatgga aacggcaacc cgttagccaa tgaggcggag
     2701 acattcacgc taccggcaga tgtgagcgca agctttactc tcggacaagg cggttccgcc
     2761 attactgaca tcaacggcaa ggctgaagtt acactgagcg gtacaaaatc cggctcctac
     2821 cccgtgacag ttagcgtgaa caattatggt gtcagtgata cgaaacaggt gactttgatt
     2881 gccgatgctg gtaccgcaaa actagcctcc ttaacctctg tatactcatt cgtcgtcagc
     2941 acgaccgagg gcgcgaccat gactgcaagc gtcactgacg ctaacggcaa cccggtagaa
     3001 ggcataaaag ttagtttccg cggaacctcc gtcacgctaa gcagcaccag cgttgaaacg
     3061 gatgatcggg gtttcgctga aattcttgtg acaagcaccg aggtcggact gaaaacagtt
     3121 tcagcctctc tggcagataa acctactgaa gtcatctcgc gattactgaa tgcaaaagca
     3181 gatattaatt ctgcaacgat taccagtctg gagatacctg aaggtcaggt catggtcgca
     3241 caagacgtag cagttaaa
//
LOCUS       DAJTNA010000286         3208 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTNA010000286 DAJTNA010000000
VERSION     DAJTNA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.292"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..469
                     /locus_tag="OO161_004028"
     CDS             <1..469
                     /locus_tag="OO161_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5464875.1"
                     /translation="VAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
     gene            697..1056
                     /locus_tag="OO161_004029"
     CDS             697..1056
                     /locus_tag="OO161_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464876.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGDTNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            complement(1049..1591)
                     /locus_tag="OO161_004030"
     CDS             complement(1049..1591)
                     /locus_tag="OO161_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCR5464877.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSKLHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNEGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPAENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSRINDRLQAIN"
     gene            complement(2195..2476)
                     /locus_tag="OO161_004031"
     CDS             complement(2195..2476)
                     /locus_tag="OO161_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5464878.1"
                     /translation="MIRDRKAEELESKGLYRRAAARWMEVMLLCTEDDDREWIKRRRE
                     TCLENVKRPPVKVEEFGDLHKAVTETQHRMGIAQPNGNAFRLNGGKRQR"
     gene            2735..3190
                     /locus_tag="OO161_004032"
     CDS             2735..3190
                     /locus_tag="OO161_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464879.1"
                     /translation="MVFAMSKSNLIAFRIPSELQDEFNRSVLASGGDKTSWLVDAIRM
                     KLGQPEKSIDSRMLGLVERMEKAAASLIAGKPNIPPKPYNETAVIKIIADTIQQGFDN
                     GRVIAERINEAGYQTKAGKAWDKDIYSAWKRQGSNAENLKAVIDCKVSV"
BASE COUNT          801 a          768 c          784 g          855 t
ORIGIN      
        1 ggtggcatgg ataaacccgg aagagagtaa atcaaaccgc gcaattggcg ttgcgctgaa
       61 tgatactgca tgtcgcgtat tgaaaaaaca aatcgggaat catcaccgtt gggtatttgt
      121 gtataaggaa agctgtacca aaccagacgg aacgaaagcg ccaacagtca ggaagatgcg
      181 gtatgacgca aacacagcct ggaaagcggc gctgagacga gcaggtattg atgatttcag
      241 atttcacgac ttgagacaca cctgggcaag ttggctggtt caagccggag tcccgttatc
      301 agtgttacag gaaatgggag gctgggagtc tatcgaaatg gttcgtcgat atgctcacct
      361 cgcacctaat caccttaccg aacacgcacg gcaaatagac tcgattctga acccatcggt
      421 cccaaatttg tcccagtcaa gaaataagga acgtactaat gatgtgtaac ttattgattt
      481 aaatggataa taggaacaat acacataggc gataagcttt gtttttgttg ccttttttat
      541 tgttcatttt ttatagatac ccccaaaagt acccccatat ctttttccgc ccctgaaatt
      601 tggttgttat tcatacagca tatcaggcgg taacgctctc cctttaaccc acttattgta
      661 tgatagtgct atcccacaat aaggaatcag ccagccatga gaatgaccag tcgcaaaaaa
      721 gagatactta gctactttga accggataac ctcgaatggg tgactggtga gattggtgcg
      781 ccgccgttcg atgtgtcggg tgtggcctac ctgcttcatg gcatggtgtc gttcgataag
      841 cgtcaccagc tcgaatcaac ccggcgcaca ctggaaagca tggttgctgg tggactgctt
      901 gagcgggtga cagtgtatga atcgcgccag atacggcgcg gcggtgatac taacgcaacg
      961 gtcgtgcgct acggtttacc cggtcagtgc gctgttatgc gcgatactgg cggcgctgac
     1021 aacgcaatat caggtgaata catgcgggtc agttaatagc ctgcaatctg tcgttgatgc
     1081 ggctcaggtc gtaaacccgg cgttcaatat ccagcatcag gaaattgtgg atctcggcct
     1141 gctcaggatt atcagactct ctggcctgta atagcaattc attgtaacca tgcaagccgc
     1201 ctgagttttc cgccggggtg gtgcctttcc cacgcaggca gctaatgggc tgattactcg
     1261 tatcgtgtgt ttgcttctcc agcacaatga cgcaatccca ccagtcgcca aagtcatacg
     1321 tgtaaaccag cttgtcaccc tcattctgga gcgccgccca tagtggcgtg attgccgcgt
     1381 tacccatgct ttcatcctca ccccatacgc gatactcgct acctggatca cctttacgcc
     1441 ctttctcgaa catatacagg tgttcgtcat tccagccaaa agctaactgg atcacggcat
     1501 gaagtttact aaaggggatt tgctctggaa ccagcacacg ccgccatata ggtggtttgc
     1561 tgtctcggat ggttatcttt agctggtaca tgcaaatcct gaataaatgc gaatcggaag
     1621 gataacgata ctgacgtact cacaattctg taaaggtgaa taaaggaaat ttgcttaata
     1681 cgcataaggt acatgtggaa taatcccata tctacctgcg cggcgctggc ttcttccact
     1741 ggtaggcagg tgctcgcatt atttcgcgga acctgtcgcg ccgggctata tcggcattaa
     1801 ctgatcgttc tatctcccgt tgatctgcct gtgtaagctg gtggcctccg tgctcaagtt
     1861 tggcgatggt caataaggct atttcgcgtg gtgtcatacg tcataccctc tggaaggatg
     1921 aaacacgccc ggattaaatt tcatttttcg cgggtataaa aaaacgatgg ggcgggcagt
     1981 cccggatagt cagggctgta gagatttgac taccccctcc ccactggatt caggttactg
     2041 ctattccgtt aagcgctgct tacggggata gctgcgttgt gattttgacc attcaggtcg
     2101 ccgctatttc ctcaccgatt gggtgctacg cgcgcgcgta cttgtcccga aataccagaa
     2161 accgccagcc agaggatgat ttccctctgg tggtctacct ttgcctcttg ccgccattta
     2221 accggaaggc gttaccgttc ggttgcgcta tccccatgcg gtgttgcgtt tcggtaacag
     2281 ctttatgcag gtcgccaaat tcctcaacct tcacgggcgg gcgcttcacg ttctccagac
     2341 acgtttcacg gcggcgcttt atccattccc gatcatcgtc ctcggtgcat aacagcatga
     2401 cttccatcca tcgtgcggca gctctccggt atagcccttt tgactccagc tcttcagctt
     2461 tcctgtccct tatcatttct gcctctcaga ttatatagat aagcaaaaag gtgttggttc
     2521 agttggttca gttggttcaa tttgtaaaga tcgttgtttt tattatgttt ttctaaaata
     2581 ttgaaccaac atcaactcat tttgaaccaa cacagcccag ctctgaacca acaacacagc
     2641 gatagctttg tgattggtcc gttattttct ggctgatatt tgagcgtata acagtgttat
     2701 acagtggtat actgtatgga tattactcac tgatatggtc tttgcaatga gcaaatctaa
     2761 tctgatcgcg ttccgcatcc catctgaatt gcaggatgag tttaatcggt ctgttctcgc
     2821 ttctggaggg gataaaacct cctggcttgt tgatgcgatt cgcatgaagc tgggacagcc
     2881 ggaaaagtct attgattcac gaatgcttgg gctggtggag cgcatggaaa aagccgctgc
     2941 gtcgcttatt gccggaaagc cgaacatccc accaaagcca tataacgaaa cggcagttat
     3001 aaagatcatt gccgatacca tccaacaggg atttgataat gggcgtgtga tagctgaacg
     3061 gatcaatgaa gcaggctatc aaacaaaagc tggtaaagcg tgggataaag atatttacag
     3121 cgcgtggaag cgtcagggaa gcaatgctga aaatttaaaa gcggtgattg attgtaaggt
     3181 cagcgtataa acatgagtgc ccattgga
//
LOCUS       DAJTNA010000287         3192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTNA010000287 DAJTNA010000000
VERSION     DAJTNA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.293"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            102..3004
                     /locus_tag="OO161_004033"
     rRNA            102..3004
                     /locus_tag="OO161_004033"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3100..>3192
                     /gene="rrf"
                     /locus_tag="OO161_004034"
     rRNA            3100..>3192
                     /gene="rrf"
                     /locus_tag="OO161_004034"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          851 a          698 c          984 g          659 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgaatcgta agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatattg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cactatgtgt tgttgggtag gggagcgttc tgtaagcctg tgaaggtgta
     1321 ctgtgaggta tgctggaggt atcagaagtg cgaatgctga cataagtaac gataaagcgg
     1381 gtgaaaagcc cgctcgccgg aagaccaagg gttcctgtcc aacgttaatc ggggcagggt
     1441 gagtcgactc ctaaggcgag gccgaaaggc gtagtcgatg ggaaacaggt taatattcct
     1501 gtacttggtg ttactgcgaa ggggggacgg agaaggctat gttggccggg cgacggttgt
     1561 cccggtttaa gcgtgtaggc tggttttcca ggcaaatccg gaaaactaag gctgaggcgt
     1621 gatgacgagg cactacggtg ctgaagcaac aaatgccctg cttccaggaa aagcctctaa
     1681 gcatcaggta acatcaaatc gtaccccaaa ccgacacagg tggtcaggta gagaatacca
     1741 aggcgcttga gagaactcgg gtgaaggaac taggcaaaat ggtgccgtaa cttcgggaga
     1801 aggcacgctg atatgtaggt gaagtccctc gcggatggag ctgaaatcag tcgaagatac
     1861 cagctggctg caactgttta ttaaaaacac agcactgtgc aaacacgaaa gtggacgtat
     1921 acggtgtgac gcctgcccgg tgccggaagg ttaattgatg gggtcagcgc aagcgaagct
     1981 cttgatcgaa gccccggtaa acggcggccg taactataac ggtcctaagg tagcgaaatt
     2041 ccttgtcggg taagttccga cctgcacgaa tggcgtaatg atggccaggc tgtctccacc
     2101 cgagactcag tgaaattgaa ctcgctgtga agatgcagtg tacccgcggc aagacggaaa
     2161 gaccccgtga acctttacta tagcttgaca ctgaacattg agccttgatg tgtaggatag
     2221 gtgggaggct ttgaagtgtg gacgccagtc tgcatggagc cgaccttgaa ataccaccct
     2281 ttaatgtttg atgttctaac gttgacccgt aatccgggtt gcggacagtg tctggtgggt
     2341 agtttgactg gggcggtctc ctcctaaaga gtaacggagg agcacgaagg ttggctaatc
     2401 ctggtcggac atcaggaggt tagtgcaatg gcataagcca gcttgactgc gagcgtgacg
     2461 gcgcgagcag gtgcgaaagc aggtcatagt gatccggtgg ttctgaatgg aagggccatc
     2521 gctcaacgga taaaaggtac tccggggata acaggctgat accgcccaag agttcatatc
     2581 gacggcggtg tttggcacct cgatgtcggc tcatcacatc ctggggctga agtaggtccc
     2641 aagggtatgg ctgttcgcca tttaaagtgg tacgcgagct gggtttagaa cgtcgtgaga
     2701 cagttcggtc cctatctgcc gtgggcgctg gagaactgag gggggctgct cctagtacga
     2761 gaggaccgga gtggacgcat cactggtgtt cgggttgtca tgccaatggc actgcccggt
     2821 agctaaatgc ggaagagata agtgctgaaa gcatctaagc acgaaacttg ccccgagatg
     2881 agttctccct gactccttga gagtcctgaa ggaacgttga agacgacgac gttgataggc
     2941 cgggtgtgta agcgcagcga tgcgttgagc taaccggtac taatgaaccg tgaggcttaa
     3001 ccttacaacg ccgaaggtgt tttggcggat gagagaaaga ttttcagcct gatacagatt
     3061 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggccg tagcgcggtg
     3121 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     3181 gggtctcccc at
//
LOCUS       DAJTNA010000288         3180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTNA010000288 DAJTNA010000000
VERSION     DAJTNA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.294"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            353..512
                     /locus_tag="OO161_004035"
                     /pseudo
     CDS             353..512
                     /locus_tag="OO161_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(730..2820)
                     /gene="iha"
                     /locus_tag="OO161_004036"
     CDS             complement(730..2820)
                     /gene="iha"
                     /locus_tag="OO161_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HCR5464880.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDMADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          795 a          835 c          734 g          816 t
ORIGIN      
        1 gttgatctga cctctcagta aatatcaata cggttctggc gagccgctta cgtgcagcag
       61 tcttatccgg cagtttaaag gggaggacgg agaccaatga ggcactggag cagattcagt
      121 gcagaggtga cctgctttat caaacttaat tgtgacaaaa tggcgattga gaaggtgcaa
      181 ttgacttatc cggattcggt cgtggcgagg tggtgcccct gcattctgct aaccgggtta
      241 tgagctagga gtcagcgata gacaaaggga cttatctgac cgggcccccg gaaaaatggc
      301 gggagagaat gataccttcc tgctgacgag accggacttt gagatgacga gcgatagtgc
      361 ggcagtgatg tacagcctga tcggtagctg caaaacttaa cggaatcgag ccggaaacgt
      421 ggctacgcca cgtgatcagt gtaatcaaca cctggcctgc caaccgcatg aaagagttgt
      481 tgccctggaa cgtcactctc tctgtaaact aatttttacc ccacgtcctt cacggtgcgc
      541 ttacactctg gacaagtatg taaatgataa gttaagaata atccgtgcat ttcctgtgac
      601 gatattagtt gttggtaacg aaatctcatt agcggatcgg aaatgcactt aacctaatac
      661 gttgccactg ttccgccagg agttgcagtg gcaacgtatt ctaccgtcag tgatagcgtt
      721 ttgttattat cagaactgat agttcagcga catccagtaa tttcgctcag gtatcacata
      781 tcctgttgtt gatgatcccg tctggaagta atcaccggca tacagcgtac tcttaccggc
      841 actgtacagg ctcacgtcac tgtaatcctt gttgagcagg ttattcaccg cagcattcag
      901 cgtcagggca tccgtcatct tccacgacag acctgcatcc accaccgtcc aggctttcag
      961 gtattctcct ttctcatcat acactttctt ctgtacagcg ctcagtgacg aataattctg
     1021 ggtgaaacgt ggtgttttcc cgcgataacg ggcacccagc catgatgcca cctcttcggt
     1081 gatctgccag ttcagtttcg cattcaccat gtgttcaggg gtataactca gcggcgcacc
     1141 tttgttatca ccatcacgtt gttcacttcg ggtccaggtg taattcagtg acagcgtgac
     1201 atcctctgac cacagcggca atgtgccggc aaattccaca ccgtgcaacc gggcctttcc
     1261 gctgtttaca tagctattgg tgttatcatt tatggaataa gagacaatct tgttggagaa
     1321 gtcagtcata aaacctgtga cattggcatt cagaccggcg gggttatcgt aatacacccc
     1381 agcctcataa ctgacgctct cttccggctt caggtcgggg ttaccaagta gatttgtttt
     1441 tccctgcccg gacacaccac taatcccttt atgtagctgc cccattctgg gtgccttata
     1501 tcccgtggtc acaccgcctt tcagcgtcca ggcatctgcc atatcccaga ccagatatgc
     1561 acgcggactg aagtgtcccc cgaattgctc atgatgttca tagcggctgc ccgcagtcag
     1621 cgcaagtgca tccgtgagat gccactcatc ctcagcaaat accgaccagc ttttctgccg
     1681 gaaagtttca cctgtgctgg caaggacaac tccgtctttc atggacgagc tctgaaactc
     1741 gccccccacc gtaaccagat gagattctcc cagaggggta agcagtaatg aattcaggat
     1801 aaggttcgat tccttaagct cccgcggctg accggcaagc ccccatttgt cgcgcttcag
     1861 tacactgcgt acgagctcac gacctttatt ttctgtctcg ttccagttca gatacgattt
     1921 ccatgttccg aaggtgaaag tatgatcata gccagctgaa attttgttcc gctcatagcg
     1981 cagggtccgg tcatatcccc ccgtcagact ccccagttgc ccatcccggt tatcataacg
     2041 ctgccgggtg gtatccatat caaaccagag cacatcctgc tccgacgcct tccagtcaag
     2101 acgtgcacca agattataat tctgtgactc cgtgggataa ggaatacgcg tggctgctgt
     2161 atcgctcagt gatgtgaccg atgagccctg acgctgttgt gtgctaccgc gtacctgcag
     2221 gctgacagaa tcatccacaa gggggccact gctccagaaa ttaaactggc tgctgttacc
     2281 ccatttgttg ctttcctgca gatttagccc tgcattgacg gaagagagcc atttgtctgc
     2341 attctttctg gtaatgatat tcaccacacc gcccatcgca tcagagccat acagtgtgga
     2401 catcggcccc ctgataacct caatacgctc aatggcggcc agagggggca tgaacccggt
     2461 attcatggca gaaaaaccgt tgggagtcac gtcactgctt ccgccctgac gaacaccatc
     2521 aatcagtatc agcgtgtaac tggctggcat tcctcggatg ctgatttcca gcccaccggt
     2581 tttacccgta ccactttcaa catccacacc ctctactgat ctcagagcct ccgccagatc
     2641 gtggtactgg ctggactgca attcctcctg gctaatcaca gaaacactgg cggctgcgtt
     2701 agtcagcttt ttctcatagc cggatgccga gacaatcatc acatcctctg cagcagctat
     2761 gctgctggct gaaaatccga gacagggaat gactacggaa gccagagtgg ttattcgcat
     2821 attactaatc tccgatcatg ttaaccggca agacaataca caaatacaaa caataatgag
     2881 aataatttat atttgccttt tacaaaaaat aaagattatt aacaaaacta aatataaata
     2941 acaactgcct cggcatgcca gttaacagct atccgtatgg cgtgttcggt tttgcgaata
     3001 aaaaatggtt actgtagaag cggatttcca ccacatgtca accatgaatg gtcagtggtg
     3061 cgagacgact gggatgattt ggtcgatgac ttcttccggt gaatactgat agaaacattg
     3121 aaatattgta agagaaagcg ttcaaaattc tgtggcacgt gaaaaattct acagcgtcat
//
LOCUS       DAJTNA010000289         3165 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTNA010000289 DAJTNA010000000
VERSION     DAJTNA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.295"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..191)
                     /locus_tag="OO161_004037"
     CDS             complement(<1..191)
                     /locus_tag="OO161_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5464881.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASPIHSSEG"
     gene            complement(327..1430)
                     /locus_tag="OO161_004038"
     CDS             complement(327..1430)
                     /locus_tag="OO161_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464882.1"
                     /translation="MNSLARRLRDYDKTDMVMGRVYSNVFDCGFIDMEQSWIQLLLIF
                     SNKNDSVSKEIKEAIENNYITYREKQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKE
                     NLRKLLQEHINMLKKDLDMRLQDAAIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSI
                     DFKQASDCFTKHKISIKIDKEPYAEGIESIPYINEGDIQADLILKDIQRIILSNLFST
                     GCSQHTVIEDFNMLIDHIKSSADLAGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKND
                     DGSMNITTESGTRKVYFLPFVNQPFSLVVKDNYFTKLIIREYDNNKLVNVTSENIKSD
                     SDKFKLTLNYELNIVFEGNADLKIAHSQRVTSE"
     gene            complement(1731..3165)
                     /locus_tag="OO161_004039"
                     /pseudo
     CDS             complement(1731..3165)
                     /locus_tag="OO161_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          974 a          526 c          562 g         1103 t
ORIGIN      
        1 atgccctctg atgagtgaat gggggaggcg tgctttgaag tgaatttgtg gaaggcttcc
       61 agtgttgaga aacatacgcc gcattctaga ttgttacact gataatactt ttgtctcacg
      121 gtgtttgaat cattttccgg acgactggtg cggatacggg cagatgcgcc acaaagcgga
      181 caacggaaca tagcgacctc tcttattgta gtgcttgcac tattctaggt tgtattctct
      241 aagatttaaa catgactttc tctatataat gatttttagc attagttaat aaacgaacgg
      301 cactctaagg agcgccgtca aagatattat tctgatgtca ctctttgcga gtgagctatt
      361 tttaaatcag cgtttccttc aaatactata ttcaactcat agttaagagt tagcttgaat
      421 ttatctgagt cacttttgat attctctgaa gtgacattta ctaacttatt attatcatat
      481 tctctaataa ttaatttagt gaagtagtta tcctttacca caagagaaaa tggttgatta
      541 acaaatggta agaagtaaac cttacgggta ccggattcag tagttatgtt catagagcca
      601 tcatcatttt ttctcattag ttctctgagg ttgggattat cgaaaagcat acgattatat
      661 tgctgaaaga tctctttact catcacaaga accagtttgc cagccaagtc agcagatgat
      721 tttatgtgat caatcaacat attgaaatct tctattacgg tatgttgcga acagccagtg
      781 ctaaataaat ttgaaagtat tattctctgg atgtccttga gaattaagtc tgcttgaata
      841 tcaccttcat tgatataagg gatggattct attccttcgg catacggttc tttatctatc
      901 ttaatagata tcttatgttt tgtgaaacaa tcgctagcct gcttaaaatc aatagatttg
      961 aaaagtgaaa gagggagcgt tttcttgatg cgtttacgaa gatgttctga tgttttacgt
     1021 gcagtgctat ctagtcgatg tacatctatg gcagcatcct gaagtctcat atcaagatct
     1081 ttctttagca tgttaatatg ctcttgaagc aactttctta agttttcttt ttttgtttgg
     1141 agatcgtcca atgttaaagt cagcttgatt ttagtgtatt cgatggagtt acaaattttt
     1201 gataatactc caatgagtcg ttgcttctca cgatatgtta tatagttgtt ttctattgct
     1261 tcttttattt cttttgatac gctatcgttc ttgttagaaa aaattaataa tagctgaatc
     1321 catgattgtt ccatatcaat aaatccacag tcaaaaacat tactgtaaac tctgcccatc
     1381 accatgtctg ttttatcata gtcacgtaaa cgccttgcta aactattcat ctacccattg
     1441 tgctccatat tttcagacca tgtccacagg cagattctaa ttaatatctt gattagttgg
     1501 cttgcattat caatttcttc agtgggagtt tcatatccac cggtttggtc aataagttta
     1561 ccctctaaaa taagtttaat gtaatgtgtc attagtttgt cagaagtatt gattcttgat
     1621 gtcagataaa gacatgtgca tatacgcatg tcagtcaacg catttatgat ggcaatattg
     1681 aaccactctc tgtctgaatt gaagtttagt tttttggata tgaaaaaaac cgggttaaaa
     1741 agctggcctt tgtaactgta tttttctacc gaatggctgt caatattcat tgagtgctgc
     1801 catcggttaa taagatcgac ggctgtatca atagtagaag gatcgcgagt ctgtgttgca
     1861 tcaataatca tcgatggaag ttcttgtagt tgcgttttgc aaaataattc aaataaatct
     1921 gaattctctg tattgcagaa acgaaagttt atgctttctt gatattgctc ccaaacagtg
     1981 gtataggttc ttaatatacg attataacgt gaacgaagcg aggcatttaa gagtgattgg
     2041 ttgtttcgga accattctgt taatattgac cattgtgaac gagtaattcc catatagata
     2101 caaagttcat tgctgataag atgttttcta ttattgataa tacgcactcc gctccaaaga
     2161 cataaatcaa aaaatctttc agaaagatta attttatcca tggaattatt tgatatttta
     2221 taaacttcat ctgaaaactc atattgaaaa ctccgttcaa ataattctgt agtgatcagt
     2281 aagaagttat tgttggtatt atcatcttga aaaaaataca aatcgcagag attattaaag
     2341 ttattaatta atgcagagat agcattgtca aaactaatga gatcagaatc ccgcagatac
     2401 atatacgtat ctgctgttat agcctgcatt gtaagactta cggaaaaatc tcctccttta
     2461 atcggagccc tataaaaaca ttgcttaagt atttttatta ataagctgtc tggtttgata
     2521 tttttaacgc taaaaagtat cttacccgtg gattctgttt cttttgtacg attatcacca
     2581 atgacaaatg acgcaaaatg attcttcttt tttagttggt aattaataag attaagagta
     2641 agttgaaaag gacggtgata taaacttaac tcatcttcct tagaataact gctagccaca
     2701 gatatgtatt ctcctttgta atcggcttgc ttgatttcta aatttgaata gttatgtttt
     2761 tgatagatag ggtataacct aagcttggct gagattttgt tacaaatatg gggcattact
     2821 atttcgaagg caatgtatcg ctttataatt atttgccgtt ttacatcatc aagaatttca
     2881 agactcacga taaagaacca aatagaaaga actatgttta tcaaaagcca taatatgctg
     2941 attagacaag ctataccata aagataactt ccaaagacag ttttaattag tacactgaga
     3001 agtataaatc ctgataagaa aagcccgctg agtccagcaa gcatgaatcc tgagtaacgc
     3061 tgataagcag tttgggctat ttttctatct gccttttttg ggaataagac acttactaaa
     3121 cctataacaa gggggtatac aataccaata attgtcaatt ggcca
//
LOCUS       DAJTNA010000290         3098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTNA010000290 DAJTNA010000000
VERSION     DAJTNA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.296"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            579..1295
                     /locus_tag="OO161_004040"
     CDS             579..1295
                     /locus_tag="OO161_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464883.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFLRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1847..2251
                     /locus_tag="OO161_004041"
     CDS             1847..2251
                     /locus_tag="OO161_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464884.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2314..2948
                     /locus_tag="OO161_004042"
                     /pseudo
     CDS             2314..2948
                     /locus_tag="OO161_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2990..>3098)
                     /locus_tag="OO161_004043"
     CDS             complement(2990..>3098)
                     /locus_tag="OO161_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5464885.1"
                     /translation="IREYIEIFYNRQRRHSRLGNISPAAFRIKYYQMTA"
BASE COUNT          870 a          639 c          700 g          889 t
ORIGIN      
        1 gattgtgggc cataaatatt ccaaaaaaat agcccgctcc gtttattacc ggaacgggcg
       61 agagcatgac attgtctgta attataatga gactgctgat tctacttatt tttaatttca
      121 agaaaagcgt tttctacaaa gaattatgag atttaatgtt ttgtttagac aatattatca
      181 ttctgttcaa taatgaaagt cttcatcaac aacaagtaaa aacaacgtcc ctctcatcga
      241 accggacttg cagatttccc gcatccggct ctcttgcaac acatcagacc ttcacgttcg
      301 ggcggttatg gggaaggtcc agcaagctgt atgagcccta actgtttgaa taccagtaga
      361 ttaccagaaa cttctatcga ttgaagacat taccaactag cgttactaaa tgttgaccaa
      421 gttttgttca cttgtctggt tttgatagta gcattctgca gagtattttg atatgataaa
      481 tatgcattaa caatgatatt attgttaatt tactagcatg aaaattaaaa gatcaacttt
      541 ttatctcgaa tattttttac attatcagtt ggtttcttat gaatgacaat tcattgctta
      601 gaaattcttc attattcatc gcctatatgg gatgtgtagg gtgggtcagt gcttattctt
      661 atggctgggg aacctctttt tactatggat ttccatggtg ggttgttggt gcaggtttgg
      721 atgatgttgc cagaagttta ctctatgcta tcatagtaat gggtatactt tttaccggtt
      781 gggggattgg tattttattt tttcttctta taaagaaaag aagtaaaata caagacttga
      841 gttttttaag actatttttt gctattaccc tactgttttt ccctgtaata ttcgaacttt
      901 taattctcaa acaatatttc atacttccat tatcactttc atgcattatt agttcattgg
      961 ttatttcaat aataattcgc atatatggac ggatcttttc tgtatcatgt ttttcagata
     1021 tcccttttgt tagagaacac cgcattaagt taattatggc tggttttttg gtttattttt
     1081 ggcttttttc ctttcttgtt ggctggtata aacctcaatt aaaaaaagaa tatcagatgc
     1141 tttgttataa taatagttgg tattatgttc ttgctcgtta tgattcacgg ctcgttttgt
     1201 ctagttcatt taaagatgac agtaacagat tcttaatatt taatacagaa cagtcgggat
     1261 tttatgaaat caatgatgtt tatgtcagaa agtaataatc ctctcccaca aagtgaatta
     1321 aacgaatata taatgcgttc gaagtatatg tattgagctg tcatactgag aaacaaacct
     1381 tagggcagca caattaaagc cagaaacaac tatgcaagaa agttactaaa taagtgctcg
     1441 gaacagtaaa acactcaggg gggactcagt ccagattatg taatcagata aattgccaaa
     1501 catcacaaat caccagcagg actgagtgat gccgagcatt gcaccaatac cccgtgatga
     1561 acgatgcctg atgcagaaag ctccctataa aacaaagaac aaaaactacg accggcgatt
     1621 caccgtcata ctgatgctgc atcgtggtga cactgtctgt cactttcatg ttgactgaac
     1681 aactctgctg cgaagaaggt gtaccgccgg cgggatttga ttgtccggcg tctagcttcc
     1741 acttccctga tggaactgaa agttgttatg gccggtcagc ttgccctgtc cgcaggacat
     1801 gaggtgctgt gacggacagc accagtaacg gaaagtgaca ataaaaatga atactgcgct
     1861 gatagttgca ctaatgtgta tgtggtatgc ggtacccgct gctgcaaaag agactttact
     1921 ggctatgcca cgtaattcaa cagaacattg ctatgcggag attaatgtgc acggaccata
     1981 tggggtgtat ttccgagtgg tcccgcatcc ccccggggga aagagctggg tcgaatgtaa
     2041 cagtgactac tattactctg ataaaccacc gggagtacag atactcggaa cccgtgcagg
     2101 gtgtcgggta tacggaatct gcgggaccac ctcgacactg catgtggctg gaagaggggt
     2161 tgtgtgtatt aaaaatatat gcagtccacg tgggatgata attcacagga tacggaaacg
     2221 tcctgtagtg gctgtaagcg atgagatgtg atcttaccca gcaatagtgg acacgcggct
     2281 aagtgagtaa actctcagtc agaggtgacc cacatgacaa aaacagtatc aaccagcaaa
     2341 aaaacacgta aacagcattc gcctgaattt tgcagtgaag ccctgaagct tgctgaacgc
     2401 atcggtgttg ctgccgcagc ccgtgaactc agcctgtatg aatctcagct ctatgcgtgg
     2461 cgcagcaaac tgcagcaaca gatgacctcg tctgagcgtg aaagtgaact ggccgctgaa
     2521 aatgcccgcc tgaagcggca actggcggtg caggcagagg aactggctat cctccaaaag
     2581 gccgcgacat actttgcgaa gcgcctgaaa tgaagtatgt ctttattgaa aaacatcagg
     2641 ctgagttcag catcaaagca atgtgccgcg tgctccgggt ggcccgcagc ggctggtata
     2701 cgtggtgtca gcggcggaca aggataagcc ctcgtcagca gttccgtcag cactgcgaca
     2761 gcgttgtcct cgcggcggcc tttacccggt caaaacagcg ttacggtgca ccacgcctga
     2821 cggatgaact gcgtgctcag ggttaccact ttaacgtaaa aaccgtggcg gcaagcctgc
     2881 gccgtcaggg gctgagggca aaggcctccc ggaagttcga cccggtcagt taccgcgcac
     2941 atggcctgag gtgtactggc aatagcggac accaccattt gttctttttc taagcagtca
     3001 tctgataata ttttatcctg aaggctgccg gggagatatt ccccagacga gagtgacgac
     3061 gctgacgatt gtagaaaatc tcaatgtatt cccgtatt
//
LOCUS       DAJTNA010000291         3092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTNA010000291 DAJTNA010000000
VERSION     DAJTNA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.297"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..196)
                     /locus_tag="OO161_004044"
     CDS             complement(<1..196)
                     /locus_tag="OO161_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5464886.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRF"
     gene            389..891
                     /locus_tag="OO161_004045"
                     /pseudo
     CDS             389..891
                     /locus_tag="OO161_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(937..1353)
                     /gene="rraB"
                     /locus_tag="OO161_004046"
     CDS             complement(937..1353)
                     /gene="rraB"
                     /locus_tag="OO161_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5464887.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1515..2519
                     /gene="argF"
                     /locus_tag="OO161_004047"
     CDS             1515..2519
                     /gene="argF"
                     /locus_tag="OO161_004047"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5464888.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2591..2914)
                     /locus_tag="OO161_004048"
     CDS             complement(2591..2914)
                     /locus_tag="OO161_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464889.1"
                     /translation="MKQPDFAKWYFYQLLKKYEGEQLYLNELGYVYGNEEKTNEIVNN
                     NPGYVVEIFEEKMGNELKIRTRMMEILRDGKINICEYINKEQLEKLNPPEDLRIAIKK
                     LGWNN"
     gene            complement(2901..>3092)
                     /locus_tag="OO161_004049"
     CDS             complement(2901..>3092)
                     /locus_tag="OO161_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464890.1"
                     /translation="SRKLQELIWDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEV
                     PVSILVNDIFFEGFFHEAT"
BASE COUNT          869 a          702 c          657 g          864 t
ORIGIN      
        1 aaaatcgttg tccgttaact tccatattag catagagata acgcttacat ttcattaatg
       61 cccatggtaa atagatacct agcgttataa tcgttaacaa cacattcacc acacaaataa
      121 gaaaatatct ctcacctgta ccatgaaaaa caaacgaatg ggacggaaca tccatttcat
      181 taataacttg agccataaaa attccctgaa ttaaaaaatc cattgatata atttatatat
      241 acagacagta aaatgttctg ccacgttaca atactcttat cagtaacagg ctggcaaaaa
      301 ctttattgct ttataagatg cttgcgcacg taagaagaga gtggtattaa acaaatgtat
      361 aattacttta tttttattgg gttaaactat gaataacatt gcgccgcaat cacctgtaat
      421 gcgtcgcctg acgctacagg ataatcctgc tattgcccgc gtcattcgtc aggtatccgc
      481 cgaatacggt cttaccgctg ataaaggcta caccatcgcc gatccgaatc ttgacgaact
      541 gtatcaagtg tatagtcagc cgggtcatgc atattgggtg gttgagtacg agggtgaagt
      601 ggtcggcggc ggtgggattg cgccattagc aggtagtgaa tcggacattt gcgaactgca
      661 aaagatgtat tttcttccgg ctattcgcgg taaagggctg gcgaaaaaac tggccttaat
      721 ggcgatggag caggcacgat agatgggctt caaacgctgc tatctggaaa cgaccgcttt
      781 ttaaaggaag ccattgcgct ttatgagcat ttgggctttg agcatatcga ctatgcactt
      841 ggctgcacgg gccatgttga ttgtgaagtg cggatgttgc gcgaactcta aaaaaatgcc
      901 cgctagtgtg tgctatcggg cattttaatc aattaattag tggcgaaccc cgtcatcgtc
      961 ttcatcgaca aaatcttcat cgtcgccgtc ttcgccatta ggatcttcaa aataagtgcc
     1021 ccaaccgtcg tactcaacgt caaatttctc cgccaatgtc atcagttgtt caacctgggc
     1081 atcgatcaga tcggcattca acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc
     1141 accatcttca acttccagct cttctggatc ggtcacttcg taaccgagtt taaacgcttc
     1201 aactgctgct ttttccaggg tttccagatc gtctgcggaa agatggtgtt cgatggtgta
     1261 cagcgcgtcc ggatcgctgc catcttccag taattcttca ataatcaaac gagtttcttc
     1321 acgctgttct tccagttgtt ccgggtttgc catggctcat tcctcttaat gtcctgccga
     1381 tactcttatt gtcacatacc actgacattg cctccacctt tgtgacaaag atttatgctt
     1441 tagacttgca gatgaataat catccatata aattgaattt taattcattg aggcgttagc
     1501 cacaggaggg atatatgtcc gggttttatc ataagcattt cctgaaatta ctcgatttca
     1561 cgccagctga actcaacagc ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa
     1621 gcggcaaaga agaagccaaa ctcactggta aaaacatcgc gctcatcttc gaaaaagact
     1681 cgacccgtac ccgatgctct ttcgaagttg ccgcatatga ccagggtgct cgcgttactt
     1741 atctcggccc aagcggcagt cagattggtc ataaagagtc gattaaagac actgcccgcg
     1801 tgctgggtcg catgtatgac ggtattcagt atcgcggcta tggtcaggag attgccgaaa
     1861 cactggcgga atacgctggc gtgccggtat ggaacggcct gaccaatgag tttcacccca
     1921 cgcagttgct ggcggatctc ctcaccatgc aggagcattt acccggtaaa gcattcaacg
     1981 aaatgacgct ggtctatgca ggtgacgcac gtaacaacat gggcaattcg atgctcgaag
     2041 ctgcggcgct taccggtctg gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag
     2101 ctgcgctggt tacggaatgc cgcgccctgg cacagcaaaa tggcgggaat attacgctga
     2161 cggaagatgt agccaaagga gttgaaggtg ctgactttat ctataccgat gtatgggtgt
     2221 cgatggggga agcaaaagag aaatgggcag aacggattgc attactgcgt gattatcagg
     2281 tgaacagcaa gatgatgcag ttgaccggta acccggaggt caaattcctc cactgcctgc
     2341 ccgcgtttca tgacgaccag acgacgcttg gtaagaaaat ggcggaagag tttggcctac
     2401 atggcggaat ggaagtgact gatgaggtct tcgaatctgc cgccagcatt gtttttgatc
     2461 aggcggaaaa ccgcatgcat accatcaaag cggtgatggt cgcaacgctc agtaaataat
     2521 tgatatcgct ataaagatgc cgggaaatac aaaaacatcc cggcatcaca ttattttatg
     2581 cagtaatagt tcaattattc caccccaatt tttttattgc tatacgtaaa tcttccggag
     2641 gatttaattt ttctaactgt tctttattaa tatattcaca tatatttatt ttcccatccc
     2701 ggaggatttc catcattctt gttctaattt ttaattcatt acccatcttt tcttcaaaaa
     2761 tctcaacgac ataacccgga ttgttattta caatttcatt tgttttttct tcattaccat
     2821 aaacatatcc taactcatta agatacagct gttcgccctc atacttctta agtaattgat
     2881 agaagtacca cttcgcaaaa tcaggttgct tcatgaaaaa atccttcaaa aaaaatatca
     2941 tttactaaaa tgctgacagg cacttcatta aatagcaact taacatcatc aaataacctt
     3001 agtattaagc gtgatttttc ttgattgctt aagttaaaat cattgacata tgcaaccaac
     3061 ttatcccaaa tcaattcctg taatttacgg ct
//
LOCUS       DAJTNA010000292         3063 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTNA010000292 DAJTNA010000000
VERSION     DAJTNA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.298"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            35..796
                     /locus_tag="OO161_004050"
     CDS             35..796
                     /locus_tag="OO161_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCR5464891.1"
                     /translation="MNNILLPPINIPCTLFETISLFDYFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     FFGQNKTLIEDLIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGIN
                     STPSTGYQPLILQSIKTKLLSSRLNKFNNFEDSFNGLGISVHNISAQKISLLSFQKYA
                     IGWSATIHFVAQDHSGLDVTDIKNKTYSKYRFFRI"
     gene            800..883
                     /locus_tag="OO161_004051"
                     /pseudo
     CDS             800..883
                     /locus_tag="OO161_004051"
                     /inference="COORDINATES: protein motif:HMM:NF023120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            880..1371
                     /locus_tag="OO161_004052"
     CDS             880..1371
                     /locus_tag="OO161_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCR5464892.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHRPWTDSDKINWYLKHQNEIKNQHPLPEGSWRTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVTNYLMIINEYLYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(1413..2363)
                     /gene="corA"
                     /locus_tag="OO161_004053"
     CDS             complement(1413..2363)
                     /gene="corA"
                     /locus_tag="OO161_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5464893.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2733..>3063
                     /locus_tag="OO161_004054"
     CDS             2733..>3063
                     /locus_tag="OO161_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464894.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTEWVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          931 a          673 c          622 g          837 t
ORIGIN      
        1 atattgagct taaatataac caaggcaaat gcaaatgaat aacatattac ttccgccaat
       61 taatattccc tgtacacttt tcgaaactat tagtttattc gattattttt ctgctgatga
      121 tatgcaatat ggtgatatgg tagaacaaga ctttctctca ctcggcctaa gcgatatctc
      181 agcaaaagtt gatccttaca gacttattaa ataccatttc cctggcccag gttctattaa
      241 tgttgctttt agtgcctcaa gttccggaac aaaaatatca caacgtgaat gcactgatat
      301 tttatttgca gaaatgaaag agctagcaaa aatgttttcc ttttttggac aaaataaaac
      361 acttatcgaa gatctgattg aacattttag atatggaaat ggcagcaatt ttcatagcca
      421 acaattaaat ttatcttttc atgaaaaaat aaataaatat ggttataata gtccaatacg
      481 aataatcaaa gaatgtatcg aaaatggcat taattcaact ccctcgacag gataccaacc
      541 tcttatcctg caaagcatta aaaccaaatt actttcatca agactaaata aatttaataa
      601 cttcgaggac agttttaatg gcttaggaat cagtgttcat aatatttcag ctcaaaaaat
      661 atctctttta agttttcaaa aatacgctat aggatggagt gcaacaattc attttgtagc
      721 acaggaccac tccggtcttg atgtaactga tatcaaaaac aagacttaca gcaaatatag
      781 attttttcgc atatagttct ttttacaacg gcataaagac tttgctttca aacctttttt
      841 caccaatttt aacactatcg aaagaatcga aaattatcta tgaaaagcaa actccagaaa
      901 attatattat gtttatttct tctatgctgc atctataatt tatggacatt gcgtcctgta
      961 cagattttgt atacatactc tgatgctggc aattcagtct ttctggtcgt tgatcatcga
     1021 ccatggactg acagcgataa aataaattgg tatctgaagc atcagaatga aattaaaaat
     1081 caacatcccc taccagaagg tagttggcgt acctggtatg tcattgatat cggtaatggc
     1141 ttcactgact ataaaaaata tatcgaaggg ccgtatgaag atctctactg tttcccgaca
     1201 attaagagca atgataattg catcgtgaca aattacttaa tgattattaa tgaatacctt
     1261 tatcgtaaca ctcatattgg tattaacgat tttactgagt atcaattaac acaagaaaat
     1321 aaaatagagc gagttttcaa tccgcatgac tttaaaaaag acaattttta gaaataagcc
     1381 ggataaaaaa atagctttat ccggcactca tattacaacc agttcttccg cttaaagtac
     1441 agatacggtg ccaggcccgc gaggatcata aagataatcg cgccagggta gccgaagctc
     1501 cacttcagtt ctggcataaa ctcaaagttc atgccatagc tggaagcaac gagcgtcggc
     1561 ggcaggaata ccacggatac caccgagaag attttgatga tgcggttctg ctcgatgttg
     1621 ataaaaccca tcgccgcctg catcaggaag ttcaccttct ggaacagaga ttcgttatgc
     1681 ggcagcaggg attcgatatc tcgcaggatt tcacgcgcct gctccagttg tccacccggt
     1741 aagcgtgctt tacgtaccag gaagttgagc gcgcgctggg tatccatcag acacaggcgt
     1801 actttccagc cgatatcttc cagttccgcc agcgtggaga gcgcctcatc gtactcatcg
     1861 ccctgatgcc cttccataat cacccggctc aactgctcca ggtcgctata gatattttca
     1921 atttcatctg ccaactgttc gattttggtt tcgaacagat ccagcagcag ctcgtaggcg
     1981 ttaccgtcta ccatcgactg gctacgggca cgcatacgat acagacgaaa agcgggcagt
     2041 tcacgctcac gcagagtaaa cagacgacca tcacggatgg taaatgccac agtggagtta
     2101 ccggcgtgat cttccgcatc ttcaaagaag aagaaggagt gaatatgcag gccgtcgtcg
     2161 tcttcaaaga aacgtgccga tgcttcgatg tcttccagtt ccgggcgggt tgccaggctc
     2221 tggccaagtt cagattgtac acgcagtcgc tcgtcgtcgt ccggttcgac aagatcaatc
     2281 catactgcat ttacaagggg ttgtgactct tcgacttcca gccgggtcag tcggttattt
     2341 tccagttgaa atgcgctcag catgaccggg actcccaatg cttaaaatat cggacagttc
     2401 ggtgggcaca cagaaacaaa ttgggtttca gaccattaaa cagcctgact cagcgcgacg
     2461 ggaaaaattg aggtcgctga caactcctaa ggctatcagc aaaaagggat agccttagga
     2521 gttgatcctg gatgacagga tagtgagcca gtatctactg ggtgtgtcca aggcgaatgt
     2581 cctcttagaa tgatcgtgcg cgcatgttac gccagcaaaa ttttgccgtc aacacgcaac
     2641 agaacaccac agaacaatag ttgaacttcg cgctttttgg tactcgtcaa ggtcgctaac
     2701 aggataaaaa aatcccacat ccaggaccta taatggcgca tcggctactc attggtaaag
     2761 gaatgatcac cttgaatctc aaacggattt ttctcaccct caccttactt cccttgtttt
     2821 ctgttgccgc tgatgattgc gcactctcag atccgacgct gaccgtacag gcgtataccg
     2881 ttaatcctca aacagagtgg gtgaaaatgt actggcaaaa agccaatggc gaagcgtggg
     2941 gaacgttaca tgctctgctg gcggatatta atagtcaggg tcaggtgcag atggcgatga
     3001 acggcggcat ctatgatgaa agctatgcgc cgctcggttt gtacatcgaa aacggtcagc
     3061 aga
//
LOCUS       DAJTNA010000293         3022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTNA010000293 DAJTNA010000000
VERSION     DAJTNA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.299"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            207..1820
                     /gene="mppA"
                     /locus_tag="OO161_004055"
     CDS             207..1820
                     /gene="mppA"
                     /locus_tag="OO161_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5464895.1"
                     /translation="MKHFVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVANQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDMDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(1871..2902)
                     /gene="ynaI"
                     /locus_tag="OO161_004056"
     CDS             complement(1871..2902)
                     /gene="ynaI"
                     /locus_tag="OO161_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5464896.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT          871 a          674 c          712 g          765 t
ORIGIN      
        1 cgtgcgaagc ctgaacgtag tgttcggtgt tctatgtgct ttgccgcatt ttggcgtcga
       61 ttatttacac taaacctgat ttaatatatt gatacataaa acattttatg cttcctgtgt
      121 cacttttttg atggaagttg tttgcatttc tttaaggcga aacaaataat tacgcatcaa
      181 ttttaatgtc ggttagaggg aaacttatga agcactttgt ttcagtcacg tgttgtgcgc
      241 tgttggtcag cagcatttct ctttcgtatg ctgcagaagt tccgagcggc acagtactgg
      301 cagagaagca ggagctggtg cgccacatta aagatgagcc tgcgtcgctg gatcccgcta
      361 aagccgtggg cctgccagag attcaggtca ttcgcgatct gtttgaagga ctggtgaatc
      421 agaacgagaa aggggagatt gtccccggcg ttgcgaatca gtggaaaagt aatgacaacc
      481 gtatctggac ttttaccctg cgcgataacg caaaatgggc ggatggcaca ccggtaacgg
      541 cgcaagattt tgtctacagc tggcagcgtc tggtggaccc gaaaacgcta tcgccatttg
      601 catggtttgc cgcgctggcg ggaatcaaca acgcacaggc gattattgat ggtaaagcta
      661 cgcctgacca gcttggcgtc accgcagttg atgcccatac cttgaaaatt cagcttgata
      721 aaccgttgcc gtggtttgtg aatttaaccg ctaactttgc cttcttcccg gtgcaaaaag
      781 ccaacgtaga aagcggtaaa gagtggacga aacccggaaa tctgatcggc aatggcgctt
      841 atgttcttaa agaacgcgta gtcaatgaaa aactggtcgt ggtaccgaat acccattatt
      901 gggataacgc caaaacggta ctgcaaaaag tgaccttcct gccaattaat caggaatccg
      961 cagccactaa gcgttacctc gcgggggata tggatatcac cgaatccttc ccgaaaaata
     1021 tgtatcagaa gctgttgaag gatattccgg ggcaggttta tacgccgccg cagctcggga
     1081 cctattatta tgcgtttaac acgcaaaaag ggccgacggc agatcagcgc gttcgcctgg
     1141 cattaagtat gacgatagat cgccgcctga tgaccgaaaa agtgttggga acgggcgaaa
     1201 agccagcgtg gcattttaca ccagatgtta ccgccggatt tacgccggaa ccttcgccgt
     1261 ttgaacaaat gagtcaggaa gaactgaatg cgcaggcaaa aactttgttg agcgcagctg
     1321 gttatggtcc gcaaaaaccg ctgaagctga cgcttttgta taacacttca gaaaaccatc
     1381 aaaaaattgc gattgctgta gcatcgatgt ggaaaaagaa ccttggcgta gatgttaaat
     1441 tgcaaaatca ggaatggaaa acctatatcg atagccgtaa caccggcaat tttgatgtta
     1501 tccgcgcctc atgggtgggg gattataatg aaccctccac tttcctgaca ttattaacgt
     1561 caacgcattc aggaaatatt tcacgcttta acaatccggc atacgacaaa gttctggccc
     1621 aggcatcgac ggaaaatacc gttaaagcgc gtaatgccga ttacaacgcg gcagaaaaaa
     1681 tcctcatgga gcaagcaccg attgcaccaa tttatcaata taccaatgga cgattaatca
     1741 agccgtggct gaaaggttat cccattaata acccggaaga tgtggcgtac agccggatta
     1801 tgtatattgt gaagcattga tgtgatggga actggcgtta cccttgtgga taacgccagt
     1861 gatgtctgat ttagcgcccc tgttccggcg gtgtaatgtt atccatatac agcgtctggc
     1921 tggggaaggc aaagtccgcg ccgtgtgact gcacaatatc gataatcttc aaataaacgt
     1981 cttgttgtgc agcaagccat tcagcccata ccgtggtttt ggtaaagcaa taaaccataa
     2041 tattcaatga cgagtcggca aactggttga aataaaccag taaggtttgt cgctggtcga
     2101 tggccgggtg atttttcagc atctcacgta cggcttcgac aataacgccc acttttgccg
     2161 catcctcata acgtaaacca atggtcgtgg taatgcggcg gttggtcatt cgtcctgggt
     2221 tttctacgct gatcgacgaa aacagcgagt tcggtacgta caatggacga ttatcaaagg
     2281 tcgtaatttt ggtaattcgc cagccaattt ccgctactgt accttcgata tttctgtccg
     2341 gtgaacggat ccagtcgcca atactgaaag gacggtcgaa atagagcatg atcccggaaa
     2401 agaaattact cagaatatct ttaccggcca taccgacagc cagaccacca ataccaccaa
     2461 aggtcagcaa gccagaaagg ctcatgccga aatgttcgcc ataaagcaga acaagcacca
     2521 caataatggt gattttgata atgcgcgaca taatccgcgc actggtgata tcgcgacctt
     2581 ttttaatctg ctgtttttca aactgattaa tcagcaaaaa tagcttaatc gtcagaataa
     2641 ccgcaatcag ggacgtacag ataaaatcga taacgcctgg ggtgataaat ttgagtttat
     2701 agttttctat aacataatta ataatgctac caacagcact gataattatg gtgtagatta
     2761 aaaattgcac cgcatggaat aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa
     2821 agctcatcag aatcaatgct gcgcagctac cgaaaataat gaccagatta agcgcattat
     2881 ttgtaaacag ttcagcgatc attgttttat caggctcctc cagataattg tcgtcatgcc
     2941 ggaaccccct ggcggggcta ttttaccgcg acaattcatt cagatcatca atagtcaggg
     3001 aaggaagtag caacattagc ta
//
LOCUS       DAJTNA010000294         3016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTNA010000294 DAJTNA010000000
VERSION     DAJTNA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.300"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            31..972
                     /gene="zitB"
                     /locus_tag="OO161_004057"
     CDS             31..972
                     /gene="zitB"
                     /locus_tag="OO161_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5464897.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(969..1688)
                     /gene="pnuC"
                     /locus_tag="OO161_004058"
     CDS             complement(969..1688)
                     /gene="pnuC"
                     /locus_tag="OO161_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5464898.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(1726..2769)
                     /gene="nadA"
                     /locus_tag="OO161_004059"
     CDS             complement(1726..2769)
                     /gene="nadA"
                     /locus_tag="OO161_004059"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5464899.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     EFIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALEP
                     LNRMLDFAATLRG"
BASE COUNT          751 a          778 c          718 g          769 t
ORIGIN      
        1 taagtgagaa taattaaggg atgactcgtc atggcgcact cacactcaca cacgtcctca
       61 cacctgccag aagataataa tgctcgtcgc ttgttgtatg ctttcggcgt gactgccggg
      121 tttatgctgg tagaagtcgt tggtggtttt ctttctggtt ctctggcatt gctggccgat
      181 gcgggtcata tgttgaccga tactgccgcc ctgcttttcg ccctactcgc cgtgcaattt
      241 tcacgtcgtc ctcccaccat tcgccacact ttcggttggc taagactgac cacgctcgcg
      301 gcgtttgtga atgccatcgc cctcgtggta attaccattt tgattgtctg ggaggcgata
      361 gaacgcttcc gcacgccgcg tccggtcgag ggcggcatga tgatggcaat tgccgtggct
      421 gggttgctgg caaatatact ttctttctgg ttacttcatc acggcagcga agagaaaaac
      481 ctcaacgtac gtgcagcggc attgcatgtg ctcggggacc tgttgggttc ggttggggct
      541 attattgccg cgttgattat tatctggacc ggctggacac ctgctgaccc cattctctcg
      601 atagtggtgt cgcttctggt tctgcgtagc gcgtggcgac tgttgaaaga tagcgtgaat
      661 gaattacttg aaggtgcacc ggtatcgctg gatatcgctg aactgaagcg tcgtatgtgc
      721 cgggaaatcc cggaagtccg caatgtgcac catgtacatg tatggatggt aggtgagaag
      781 ccggtgatga cgctgcatgt gcaggtgatc ccgccacacg atcacgatgc cttgttggat
      841 cagatccaac actacctgat ggatcactat cagattgagc acgccaccat tcagatggaa
      901 tatcaacctt gtcatgggcc agactgccac ctgaatgaag gggtgtctgg ccattcacat
      961 catcaccatt aatgggacag cgcgcatgag cctctttcac gtgcgctgtt gatccacatc
     1021 cgactgccgt tgagcgcaat aaaggtcagg atgatgtact ccagagacat ggcgtaaacg
     1081 ccctgaagtg caaaaataac gacgctaatc acgttaataa tcacccacaa cagccagttt
     1141 tccacatact tacgcgtcat cagaagcatt gccacgatag ataacaccat catgcatgaa
     1201 tcccagaacg ggaaagcgtc cggttgcagt tcaggcatca caacctgtaa tcctaatgct
     1261 tgcatgatca tgactgccac gcgggtcaaa aatgcaaaca ccggattgat aaataccgtc
     1321 atcagaccaa tcgaaacaac gcaaaccgcc aaccagctga gcgccttcgg caatggtaac
     1381 cagcgaattt tcaactccgc ctcgttctga ctggtttgtc gcgaccacgc ataccaaccg
     1441 taaatattcg cggcaaagaa aaacacctgt aatagcaggc tggcatacag ctgaatctga
     1501 aagaaaataa tgccaaacaa ggtgacgttg atcaggccaa agaagtagtt gctgatcttc
     1561 tccagactgg caaggccaat acacagcaac ccggcgatcg tgcctaccgc ttcgatccat
     1621 gagagatcat aaccgcctgc tcctattggt atatgtacca ggatattctg cacactaaaa
     1681 aaatccatct ttcccccaaa gcgtaacgtt acgccttatt attcgttatc cacgtagtgt
     1741 agccgcaaaa tccagcatac gattgagcgg ctccagcgcc ctctctcgca gcctttcatc
     1801 aacatgaacc tcgtgattgc ttccttcctg ttctaatgcc tctgcgatgg cctgaaggcc
     1861 attcatggcc atccacggac aatgcgcgca gctgcggcag gttgcaccct cacctgcggt
     1921 tggtgcttcc agtaactctt tatctggcac cgcctgctgc attttgtaga aaataccccg
     1981 atcggttgcc acaataagcc tctgatgtgg caatgttttc gcagcagcga tcagttgact
     2041 ggtggaaccg accgcatccg ccatatcgac aatagcttgt ggtgattctg gatgcaccag
     2101 tatggcagca tccgggtatt cttcttgcaa gcgggttaac gcctgagtct taaattcatc
     2161 atgcacaata caggcaccct gccagcatag aatgtctcca cccgtctgtt tttgcacgta
     2221 acgccccaga tgtttgtcgg gtgcccagat gattttttca cccaaactat caagatgatc
     2281 aataaattcg acggcaatgc ttgaagttac cacccaatct gcgcgcgctt ttaccgcagc
     2341 agaagtgttg gcgtagacga cgacagtacg atcgggatgg gcatcgcaaa atgcgttaaa
     2401 ttcttcaaca gggcagccga gatccagtga acattcagcc tgaagtgtcg gcatcagaat
     2461 tgttttttcc ggactgagaa ttttggcggt ttctcccata aatctcaccc cagcgactaa
     2521 caaagtagaa gcgggatgct ttgcaccgaa gcgcgccatt tccagagaat cagaaataca
     2581 gccaccggtt tcttctgcca gttgttgaat ttcgggatcg gtatagtagt gggcaaccat
     2641 caccgcatta cgttctttta gcagacgttt tatcttctcg cggtaatacg ctttttcatc
     2701 aatgcttaac ggcgtcggct tcggggggaa aggataaatc gccgtgtctg gatcaaacat
     2761 tacgctcatc ttaccatctc gttttacagg cttaacgtta aaaccgacat tagcgtaata
     2821 ttcgctgtta tcggtgtaat ttgttttata tgctaaacaa gatagtcgaa tggtggggaa
     2881 aagtcactcg aattacgcct aattccaccg acgtagagtt gcttgcacag taagctctga
     2941 cgatgtcatc aggtttacaa agtgaaattg aggatgcctg aatagcaaaa aagcgccttt
     3001 agggcgcttt tttaca
//
LOCUS       DAJTNA010000295         3010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTNA010000295 DAJTNA010000000
VERSION     DAJTNA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.301"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..218
                     /locus_tag="OO161_004060"
     CDS             <1..218
                     /locus_tag="OO161_004060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5464900.1"
                     /translation="RVAVVADVCGVSQRAIYKWMDNGKLPRTEYTGETNYAEKIALAS
                     NGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            215..640
                     /locus_tag="OO161_004061"
     CDS             215..640
                     /locus_tag="OO161_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5464901.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKSYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDALKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            712..1782
                     /locus_tag="OO161_004062"
     CDS             712..1782
                     /locus_tag="OO161_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR5464902.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     IPQTAKSPAERKRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTASPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDSEIPPGFRG"
     gene            1823..2245
                     /locus_tag="OO161_004063"
     CDS             1823..2245
                     /locus_tag="OO161_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5464903.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRILSFYQGNFQEVMA"
     gene            2242..2547
                     /locus_tag="OO161_004064"
     CDS             2242..2547
                     /locus_tag="OO161_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCR5464904.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
     gene            2534..2989
                     /locus_tag="OO161_004065"
     CDS             2534..2989
                     /locus_tag="OO161_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5464905.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSDPDVLALLKELEVKDKRIAELTDALTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
BASE COUNT          851 a          646 c          860 g          653 t
ORIGIN      
        1 ttcgcgttgc tgttgtggcc gacgtttgtg gtgtcagcca aagagcaatc tataaatgga
       61 tggataacgg aaaattgcct cgcacagaat ataccggcga aacaaattac gctgaaaaaa
      121 tcgctcttgc atcaaacgga ttattttctg ccgatgcaat tttaactatt ggcaggaata
      181 aaactactac gaaaaagctg atgggagttg attcatgaaa atcaagcatg aacacatccg
      241 catggcgatg aatgcctggg cgcatccgga cggcgaaaaa gtaccggctg cgaaaattac
      301 caaatcgtat ttcgagctgg gaatgacgtt cccggaactg tatgacgaca gccatccgga
      361 agccctggct cgcaatactc agaaaatttt ccgctgggtg gagaaagaca cccctgatgc
      421 gcttaaaaaa attcaggcgt tgttaccagc tatcgaaaaa gcaatgccac ctctgctggt
      481 ggcccgaatg cgcagccata gctcagccta ttttcgggaa ctagtggaga cgcgggaacg
      541 actggtgaga gacgctgatg attttgtcgc agtggcgatc gctggtttca accagatgaa
      601 tcgtggtggc cctgcaggaa atattgtggc tgtgcattga ctcgcaatat tcataccgga
      661 tcacttccgg caatttgtga gtaaaaagat tcggtatcaa aagaggtgag tatggctaac
      721 gcctggctca gattatggca tgacatgcca aatgacccta agtggcgaac aattgccagg
      781 gtgtcagggc agccaattgc aacagtgatg gcagtgtata tccacctcct ggtgagcgcg
      841 tcacgaaatg tcacgcgagg tcacattgat gtcacgacag aagatttggc aagtgcgctc
      901 gacgtgacag aagaggtaat tgattcaatt ttgcagacga tgcaggggcg ggtacttgat
      961 ggtgatttaa tcactggatg ggaaaaacgc caggtgctga aagaggacaa cggcaatatt
     1021 ccgcaaaccg caaaatctcc tgcagagcgc aagagggcgc agcgagagag ggaaagaaag
     1081 cgggaacaaa atggcgattg tcacggcgcg tcacgatatg tcacgcacat gtcacgacga
     1141 gtcacgacag ataaagatac agataaagat acagatcaag aagatcaaaa cactatggtc
     1201 catggcgtaa aaaacgccac gaaccaggca ggggatgttc agaccgtcaa tcctggtcag
     1261 ccagcaggca cgacaccgga agccgattca gcgtatgcgc tgaaagccga ttcgggcgct
     1321 gtgcagcagg tgatgaccgc aagtccggag caatcacacc aactgcagca gcctgaagcc
     1381 gattccgcca ttcagcggga agccgatcgg gtagtcccgg aaaacaccgg gcagtctgtg
     1441 ggacgagtgg attatccgga tgtgttcgaa caggtctggc gggagtaccc gttgcgtgcc
     1501 ggggcaaacc cgaagaaatc cgctttcagt gcctggaagg ccagattacg cgagggggtg
     1561 ccaccagagg ccatgctgga tggcgtgagg cgttacgcaa gatacctggc ggctaccggg
     1621 aaaacgggaa cggaatttgt tcagcgagcg acgacgtttt ttggaccgga ccggaatttt
     1681 gagaacccct ggttgctccc ggtaagcggc acgaacaacc agcgttgtgt gaatcatatt
     1741 tctgaaccgg atagcgaaat tccgccgggc ttcagggggt aagtgttaat ttctggtcat
     1801 gaggtaattt tcaggagggc ttgtggcaaa agtttttaca caagaagagc gggaaaaaat
     1861 taaagggcag gttcttgaac tcgtacgcca gagtgggcgc gagacgttac gacaactgga
     1921 agctaaaact ggggcaacaa gatatctgat gagcgttctg gccagagagc tggttgccag
     1981 tggcgatgta tacaactctg gttacgggtt attcccgtct gaacaggcgc gtaaggactg
     2041 gcaaaatgcc cgtaaaaagc tctcaagggc aaagctgaag aaaccatctg cggttgatcc
     2101 ggaccttatc tggtcattac ctgacggaga aatacgtcgc tacgacagtc gcctaaacat
     2161 aatctgtcgc gagtgccgga agagcgaagt tatgcagcgc atattgtcgt tttatcaggg
     2221 taattttcag gaggtgatgg cgtgagggtg agagtctata tcgccggtcc aatgaccggg
     2281 tataaaaatt tcaaccgtga ggcgtttcac aatgcggaag aggaactgaa acgggaaggg
     2341 cataccgtct taaacccggc agtacttccg gacgggctga cacagccgca gtacatggat
     2401 atctgcatgg cgatgattcg ttgtgtggat gcgatttaca tgctgaaagg ctggcagcgg
     2461 tcagcaggcg ctaaggcaga actggcgctg gcggagaaac tggggcatgc ggtgattttt
     2521 caggagacag ccagtgtgca agactaatta ccaggcatta cgtgaacgtt attcaccaat
     2581 tcaggtgccg gaatgtcctg tttgtggcga tgaaatgtcg atacagcgca tattttccag
     2641 aacgcatatt gtgtatgcct gcacaggtga gggggatgat ggatatttta aaactggtcg
     2701 aacttttgcg gatgagcatt acctgaaatc gcgcgtaacc gtcgttgatg ttagcgatcc
     2761 tgacgtactg gctttactga aagagctgga agttaaagac aaacgcattg cagaattgac
     2821 agatgcgctt acacaaatga ttaatgcgca caaaaccaca attcgttttg gtcatgaacg
     2881 cataactgaa tgtggtggtg attgcgactc gccggaaaag atgatttcag aaaatccgga
     2941 tatcagaatg gcagaggctg ttttgagagc aggaataaaa actgaataat taaatttagc
     3001 agtaagcgtg
//
LOCUS       DAJTNA010000296         2962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTNA010000296 DAJTNA010000000
VERSION     DAJTNA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.302"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(57..897)
                     /locus_tag="OO161_004066"
                     /pseudo
     CDS             complement(57..897)
                     /locus_tag="OO161_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(1319..1471)
                     /locus_tag="OO161_004067"
     CDS             complement(1319..1471)
                     /locus_tag="OO161_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5464906.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
     gene            complement(1674..2081)
                     /locus_tag="OO161_004068"
     CDS             complement(1674..2081)
                     /locus_tag="OO161_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5464907.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            2158..2386
                     /locus_tag="OO161_004069"
                     /pseudo
     CDS             2158..2386
                     /locus_tag="OO161_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            2370..2792
                     /locus_tag="OO161_004070"
     CDS             2370..2792
                     /locus_tag="OO161_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464908.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVVVH"
BASE COUNT          762 a          652 c          737 g          811 t
ORIGIN      
        1 tgagggcagg tgtaaaacaa taatcaagca tggggttcat atccatagct ccatttttat
       61 tttaaggaaa gcaactgctc tacggtcatg tttttaattg cgccccggtt tacaagagtc
      121 cagccctgtt tttccagata aaaccggaaa gtctccaggg tacagaccag tgcgccatca
      181 ggaacggttt cggtgaattc gacattgccg aatttgtcga agtgaacaac cagagtgcga
      241 ccatcacccg gaatcatctt gtcagcaggt ggggtgttat tctggcgcag ttcggcctcc
      301 atgcggtcga actcagcaat gtaggcttcc ttgaatgtgg cggctttttt gcccgtgaag
      361 cccatcacca ggaaaacgaa gccgtttttg gtgatttggt aggcgttata agaattgccg
      421 cggtgttcga atttaacccg cgaaaagttg ctggttaaaa atagttcaga acattcgagg
      481 gattctattt tttgaacaac atggtgatgc tgcttgccaa aaaactcagc aatcgaaata
      541 gaagtagtga cggcgcgacc attttcgatg gttacgtcag ggtgagaaag ggtagtagcc
      601 ataatgacat cctcaagtga taagttaatt aactcaccag cagaggtgct aatctcatgg
      661 gtggtgagac gcacagggtt agcactaccg gtcactcgag aacccggcca gccttgcggc
      721 tgccccgcac gcctcaccat aatttgaatg tggctgtgca ttacgcataa aaaaaaccgc
      781 ttcagcgcgg ttatgcgctc gagtaacttt cggtgtgcta atcccagcgc ccgttttatg
      841 aggtgcaggt gcattataat tccacccgtt ctggttttca atagctacat tcaacatttt
      901 ctctttcctt tcatcaccga agtgaatttt gtgatgcggt gcctagtgcc tccaggtgac
      961 gttaaccagt taacaattac agtcggcttt cccacccaaa ccaataagga ctaacatgac
     1021 ttttaactgt gccgcgtgcg cttagccgca ttcaccgcat cacaaaattc actttaaaaa
     1081 gggcggacat cagccagcaa ttaaaccgat gccgccaact ggtacttcac acagcaatgt
     1141 cgttatttac aaccggaagc gcactcccac catttaaatt tcacagacaa gaccgactct
     1201 ttatgaatac cggaaatgcg ccttcgtgtt gtgcccggtt ttatttcacc acctccgggc
     1261 tttggtggcc tcggctatac ctctacagcg agaatattga attaatccaa taaatggatt
     1321 agctggtatt tttggcaagc cagcgacgtg cgccagcttc ggttttaaac gttttgcttt
     1381 tggtatacgt catggcggtg aatgtgccgt cctgattggg aaacacgcca cacactaggg
     1441 attcgttgtt gccaagatcg atagtatcca tgctgacctc atttcccctt aacgccgggg
     1501 tagcggaact gtttgctgag aacaccgtgc ggtgtcttga tgaatgaaat ttagaataac
     1561 ctaaggtgtt gggttaagat tttatgtaga aaaacctaag tttttttgat gtaaaaaaca
     1621 caagtgtttg aaagtttgtg ctttttatta cagggtgtgg agaaaaaagg ggattatttg
     1681 tttgcgcttc ttttgcgagc tttgagtagt tcttcaaaaa gtttgttgaa attttcaact
     1741 cgagcacgca tctctgacaa cagggccttt tgctctgact caggcagtgc gtcgaacagt
     1801 tgaagtaact ctttttgatc ttctgtcaga atggctggct gattatccgg gatcggttcg
     1861 cctggttgtt tatcttcatc cccaaaaaga agccaagtcg gtgagcactg aagcgcttgg
     1921 ctcagtgcga ataatctttt ccccgccggc tgtgtttcat ctctttccca ttgagaaatt
     1981 gttacgtgag ccactttgac cagcttacct aatgcggcct gagacagttt taatttttta
     2041 cgcctatgta agaggcgagc accgaaggtt tcgtttttca tattagggaa ttctaatttt
     2101 tcttgactta ggtttctcta cgagctagtt tccttaggaa aatctaagga gctcgatatg
     2161 ttgaaaattg atgctatagc gttttttggc agcaaaacaa agcattgcca atgccgcagg
     2221 agttaggctg gcaagcattg ctgcatgggg ggaactggtt cctgaaggtc gcgcgatgcg
     2281 cctgcaagag gcatctggcg gggaacttca gtacgacccc aaagtttatg acgaatatcg
     2341 taaggcaaaa cgacctggga aggtgattca tgaaaatcag gcatgagcac atcgaatcag
     2401 tgctgttagc cctggcagcc gaaaaagggc aggcgtgggt cgctaacgca attactgaag
     2461 aatatctgcg gcaggggggc ggcgaattgc ctctggtacc aggcaaggac tggaacaatc
     2521 agcagaatat ctatcaccgt tggttgaaag gtgaaacgaa agcgcaaagg gaaaaaattc
     2581 agaaactgat ccctgcggtt ctggcaattc ttccgcgtga gctgcgtcac cgactctgta
     2641 tcttcgatac cctggaacgc cgtgcattac tggcggcgca ggaagcgttg agtacggcaa
     2701 ttgatgcgca tgatgacgca gttcaggccg tttaccggaa agcacatttc agcggtggtg
     2761 ggtcgtccga cgattctgtc gttgtgcatt gatgtttatg ccgaacctcc tctggttctg
     2821 ttgattgggg aatcacagat tatatccgga ggaaggttcg gcaccagacg aggtttctat
     2881 atatgagcat gaaactaatg gcaaaagcaa tggaaattaa agtgggtaat ccattgcgta
     2941 agctggttct gattaagctg gc
//
LOCUS       DAJTNA010000297         2939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTNA010000297 DAJTNA010000000
VERSION     DAJTNA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.303"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..409
                     /gene="speG"
                     /locus_tag="OO161_004071"
     CDS             <1..409
                     /gene="speG"
                     /locus_tag="OO161_004071"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCR5464909.1"
                     /translation="IHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQ
                     GKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFIN
                     GQYRNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(412..1122)
                     /locus_tag="OO161_004072"
     CDS             complement(412..1122)
                     /locus_tag="OO161_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5464910.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            1230..1535
                     /locus_tag="OO161_004073"
     CDS             1230..1535
                     /locus_tag="OO161_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5464911.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQSTQDGAPAEPQ"
     gene            1734..2939
                     /locus_tag="OO161_004074"
                     /pseudo
     CDS             1734..2939
                     /locus_tag="OO161_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT          784 a          731 c          679 g          745 t
ORIGIN      
        1 tattcacgat cagagcgaac gacgctttgt ggtggaatgt gacggcgaaa aagccggtct
       61 ggtggagctg gtggaaatta accatgtaca tcgccgggca gaatttcaga taattatctc
      121 cccggagtat caggggaaag gtctggcaac ccgtgccgcc aaattagcaa tggactatgg
      181 cttcaccgtt ctcaatctct ataagctgta tctgatcgtc gataaagaga atgaaaaagc
      241 gattcacatt taccgcaagc ttggctttac ggttgaaggt gaattgatgc atgagttttt
      301 tattaatggt caatatcgta atgccattcg catgtgtata ttccagcatc agtatctggc
      361 agagcacaaa acaccaggtc agactctcct gaagccgacc gcacaatagc attaataata
      421 atcgatcgta tttttgatgg tgtaaacccg ttcgacggcg ggttttactc cttcatcaac
      481 aatgatcagc tgacagtcca ccggattggc gtgactgtca tattcacagc tctgttttac
      541 attaccaact cgttgattat tcagtaaagt aaccgctgtg taatctaatt ttttgatcgg
      601 gtccgttgat ggcgtggcgc tgacagataa tgttttgtcg ttacttttcg tggttttacc
      661 cagtggataa ccctgatcat catagcgata ttccatctgc atttgtttgc tgctggcttt
      721 aatcacgaag ccattatcat cggtttccca actcaccccg gcagaaggta attctgctag
      781 ctggcatttt ccctgtaaac gtactctctt ctccagcgtc tcggcatcac ggtaataatt
      841 ggcgtccagt accagagcga ccacggtatt attttccaga tccagtaatt cgagtgaatc
      901 aaaacagcct tcttccgaca aagtcccaga aacacgtttc gtcacttcac cttgctcatc
      961 cattaatgtc tgagtgaaat cttttaccgg accacgcagc ggatcaaaat cgaattcatt
     1021 agagaaactg gccatctcag gggtaaatga aagtgttacc tctgtgcggt cacatcctgt
     1081 gaggaatatc gcgagtaagc atggtagtaa tttgtatttc acaacagtca ccagagagta
     1141 gagatgattc tcaatcatag tagcaaatac agtactttac acgttaaatg ctatgcttaa
     1201 agaagttatc ttcgcgtaag gagcttatga tgaaactctc aacctgctgt gccgcgcttc
     1261 tgctcgccct cgcctcaccc gcggtactcg ccgcgccggg atcctgtgag cgcatacaga
     1321 gcgatatatc acagcgcatt atcaataatg gcgtacccga aagcagcttc acattaagta
     1381 ttgtacccaa tgaccaggtt gatcagcctg attcccaggt cgtcgggcat tgtgctaatg
     1441 atacgcataa aattctctac acccgcacca ccagcgctaa cgtctctgct cccgcgcagt
     1501 ctacccagga tggcgcgcct gccgaaccgc agtaatacat tatcgactga acgccggata
     1561 tgacacatcc ggcgatttga acatacaaca taatcccacc ttattactca tacccttcta
     1621 ttgatgtgga ttaataatta ttaacccaaa atgggtagac ctcctctatt gttagcgcgc
     1681 taaatattca atacataaac atttatataa cgataaagaa cagggagtga gttatgtcca
     1741 aaaatgaccg aatggtgggc atcagccgca gaacactcgt taaatcaaca gcgataggtt
     1801 ctctggcgct ggctgcaggc ggtttttctt taccgttcac cctgcgcagt gcagcggcaa
     1861 cggtacaaca ggccagcgaa aaagtgatct ggggtgcctg ttccgtcaac tgtggtagcc
     1921 gctgtgcact tcgtctacat gttaaagata atgaagtgac ctgggtggaa actgacaata
     1981 ccggcagcga cgagtacggc aaccatcagg tacgcgcctg tttgcgcggt cgctccatcc
     2041 gccggcgtat taatcatccc gatcgcttga attacccaat gaaacgcgtg ggtaaacgcg
     2101 gtgaaggcaa attcgaacgg attagctggg atgaagccct ggatactatc gccagtagcc
     2161 tgaaaaaaac cgtcgaacaa tatggcaatg aggctgtata tattcagtac tcttcgggga
     2221 ttgttggcgg caatatgacc cgctcttcgc catcagcctc ggcggtcaaa cgcctgatga
     2281 actgctacgg cggttcactc aatcagtatg gctcctacag caccgcgcaa atctcctgtg
     2341 ccatgcccta cacctacggc agtaatgatg gcaacagcac cacggatatt gaaaacagca
     2401 agctggtcgt gaagatgaat ggcttcctat tcgcccgggc accgatgccg cgctggttgc
     2461 gggtattgcc tgggtattga ttaacgaaaa tctcgttgat caaccttttc tcgataaata
     2521 ctgcgtcggc tatgacgaaa aaaccttacc cgcagatgca ccaaaaaatg gtcactataa
     2581 agcctatatt cttggtgaag gtgatgataa tacagcgaaa acgccgcagt gggcttcgca
     2641 aattaccggt atcccggtgg accgtatcat caaactggcg cgtgaaattg gcacagcaaa
     2701 acccgcttat atctgccagg gctgggggcc gcaacgccag gcaaacggcg aactgactgc
     2761 acgcgctatt gctatgttgc ctattttgac gggcaatgtc ggcatcagcg gcggaaatag
     2821 tggcgcgcgt gaatcgacct ataccattac catagaacgc ctgccggtgc tggataatcc
     2881 ggtcaaaacg tcaatctcct gcttcagctg gacagatgct atcgatcatg gtccgcaaa
//
LOCUS       DAJTNA010000298         2929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTNA010000298 DAJTNA010000000
VERSION     DAJTNA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.304"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..282)
                     /locus_tag="OO161_004075"
     CDS             complement(<1..282)
                     /locus_tag="OO161_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5464912.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMVED"
     gene            complement(282..611)
                     /locus_tag="OO161_004076"
     CDS             complement(282..611)
                     /locus_tag="OO161_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5464913.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(608..>2929)
                     /locus_tag="OO161_004077"
     CDS             complement(608..>2929)
                     /locus_tag="OO161_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5464914.1"
                     /translation="GGQNPWLILLQQGGQVKDSFGGMIPMFRGLAGAITLPMVGVTSL
                     AVATGALVYAWYQGDSTLSAFNKTLVLSGNQSGLTADRMLTLSRAGQAAGLTFNQARE
                     SLAALVNAGVRGGEQFDAINQSVARFASASGVEVDKVAEAFGKLTTDPTSGLMAMARQ
                     FRNVTAEQIAYVAQLQRSGDEAGALQAANDIATKGFDEQTRRLKENMGTLETWADKTG
                     KAFKSMWDAILDIGRPESSADMLASAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQ
                     GAWDDRENARLGLAAATLQSDMEKAGELAARDRAEREASQLKYTGEAQKAYERLQTPL
                     DKYTARQKELNKALKDGKILQADYNTLMASAKKDYESTLKKPSGVKVSAGERQEDRAH
                     AAMLALETELRTLEKHSGVNEKISQQRRDLWEAENQYVVLKEAATKRQLSEQEKSLLA
                     HEKETLEYKRQLAELGDKIEHQKRLNELAQQAARFEQQQSAKQAAISAKARGLTDRQA
                     QRESEEQRLREVYGDNPAALAKATSALKNTWSAEEQLRGSWMAGMKSGWGEWAESATD
                     SFSQVKNAATQTFDGIVQNMAAMLTGSEQNWRGFTRSVLSMLTEIFLKQAMVGIVGSI
                     GSAIGGAVGGGASASGGTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEA
                     TSRIGVGNLYRLMRGYATGGYVGGTGSPAQMRRSEGIRFEQNNNVVIQNDGTNGLPGP
                     QMLKAVYDMARKGARDEIQAQMRDGGLFSGGGR"
BASE COUNT          557 a          998 c          699 g          675 t
ORIGIN      
        1 atcttccacc ataccggtga ccataccgta caggttagaa accgtcagcg tggggcgcgt
       61 actggtgcct ttgccattca gttcaaaacc actcccctga atgggatacg gctgatactg
      121 tcgcccctgc caggtgaccg gctcaccttt ttcgttctgc tcattacaga aaaaataacg
      181 ttctccaccg acctctgtca gatcgatttc ccagagcacc acgctggccg actgctccgc
      241 acgggtgcat tcattcagtg tttcctgccg gatatcctgc atcagttcac cacctgttca
      301 aactctgcgc tgaactcaac acgcagcata ctgacccgcg acgaccattt tgcgcaggtc
      361 acctttatct gccgccactc ataaggcggc gtccacagaa aggatttcca gcccccgtgc
      421 tcagccagaa acgactcaag cgccgtggcc tcccaacggg gaacagaaag cgtcacgctg
      481 tacgttttca ggtcggcatt cagcccggca ggcgctcgct gggaatagcc atcaccaaag
      541 cgcactttcc tgacggaagg ggctgaagcc acatccatac cgggtttcac tttccagcgg
      601 aaggtcttca tcgtccacct ccggagaaca gaccaccatc gcgcatctgt gcctgaattt
      661 catcacgggc acccttgcgg gccatgtcat acactgcctt cagcatctgt ggacctggca
      721 gaccattcgt accgtcgttc tgaatcacca cgttgttgtt ctgctcaaat ctgataccct
      781 ctgaacgccg catttgcgcc ggacttccgg tgccaccgac ataaccgccg gtggcatagc
      841 cgcgcatcag ccggtaaaga tttcccacgc caatccggct ggtcgcctcc ttcgtgaaga
      901 caaactcccc gcgatgaaca atccccgcag gttcatattt accccccgtc cccgtaaatc
      961 ccccggtcgc gaaatggaag ttcgccgccg cagcctgaat ggctgtaccg cctgacgcgg
     1021 atgcgccgcc accaacagcc ccgccaatgg cgctgccgat actcccgact atccccacca
     1081 tcgcctgctt cagaaaaatc tctgtcagca tggacagcac agaacgggtg aaaccacgcc
     1141 agttctgttc gctgccggtc agcatcgctg ccatattctg tacaataccg tcaaaggtct
     1201 gcgtggccgc gtttttaacc tgcgaaaaac tgtccgtcgc actttccgcc cactcgcccc
     1261 agccggactt catcccggcc atccagcttc cacgaagctg ctcctccgca gaccaggtgt
     1321 tcttcagtgc agatgtggcc ttcgccagcg cagccggatt atcaccgtac acctcacgaa
     1381 ggcgctgctc ttccgactcc cgctgcgcct gacggtcggt gagtccgcgt gcttttgcgc
     1441 tgattgccgc ctgcttcgcg ctctgctgct gttcaaaccg cgccgcctgc tgtgccagct
     1501 cattcagccg tttctggtgt tcaatcttgt cgcccagctc agccagctgg cgtttgtact
     1561 ccagcgtttc tttctcatgg gccagcaggg atttttcctg ctcagataac tgccgtttcg
     1621 tggcggcctc tttcaggacc acatactgat tttccgcttc ccataaatcc cggcgctgct
     1681 ggctgatttt ctcattcaca ccgctgtgtt tttccagcgt cctgagctcg gtttcaagcg
     1741 ccagcatggc tgcatgcgcc cggtcttcct ggcgctcacc ggcagacacc ttcacacctg
     1801 acggcttttt cagcgtcgat tcataatcct tttttgccga cgccatcagc gtgttgtaat
     1861 ccgcctgcag gatttttccg tctttcaggg ccttattcag ctctttctga cgggcggtat
     1921 atttatccag cggcgtctgc aggcgctcat acgccttctg cgcctctccg gtatacttca
     1981 gctgtgacgc ctcacgctca gcccggtccc ttgccgccag ttcaccggct ttttccatat
     2041 ccgactgcag cgtggccgct gccagaccca gacgggcatt ttcccggtca tcccatgccc
     2101 cctgaaggtt cgcacgaaaa gaggaggtct ttccccggcg ctggctccgg ctctggtacc
     2161 actgccattt tttatccgcc tcatcaaatg ccttctgcgc actggcgagc atatccgctg
     2221 aggattcagg acgaccgata tccagaatgg catcccacat cgatttgaat gccttccctg
     2281 ttttatccgc ccaggtctcc agtgttccca tgttttcttt caggcgacgg gtctgctcat
     2341 caaagccttt cgtggcgata tcgttcgccg cctgcaatgc cccggcctcg tctccggaac
     2401 gctgcagctg tgcaacatac gcaatctgct ctgccgtcac gttacggaac tggcgcgcca
     2461 tcgccatcag tcccgacgtc gggtcagtgg tcagcttccc gaaggcttca gcgactttat
     2521 ccacctccac accggatgca gacgcaaaac gcgcgacact ctggttgatg gcatcaaact
     2581 gttcaccacc acgcacaccg gcattcacca gggctgccag tgactctctc gcctggttaa
     2641 acgtcagccc tgctgcctgc ccggctcttg agagagtcag catacgatcg gcagtcagtc
     2701 cggactgatt accggaaaga accagggttt tattaaacgc tgaaagcgtg gaatctccct
     2761 ggtaccaggc gtacaccagc gcacctgtcg ccaccgccag cgaggtgacc ccgaccatcg
     2821 gcagggtgat cgcaccggca agccccctga acatggggat catcccgccg aaggagtcct
     2881 tcacctgacc gccctgttgc agcaggatca gccagggatt ctgaccacc
//
LOCUS       DAJTNA010000299         2922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTNA010000299 DAJTNA010000000
VERSION     DAJTNA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.305"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..345
                     /locus_tag="OO161_004078"
     CDS             <1..345
                     /locus_tag="OO161_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HCR5464915.1"
                     /translation="RKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGER
                     SCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLRK
                     DIMRRLTQDVCE"
     gene            complement(411..2789)
                     /gene="ppsA"
                     /locus_tag="OO161_004079"
     CDS             complement(411..2789)
                     /gene="ppsA"
                     /locus_tag="OO161_004079"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5464916.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSDVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKVLLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          707 a          796 c          724 g          695 t
ORIGIN      
        1 cgcaaaaaag atattattgt ccgcggcggc gaaaatatta gcagccgtga agtggaagat
       61 attttattgc agcatcctaa aattcacgat gcctgtgtgg ttgcaatgcc cgatgaacgt
      121 ttaggtgaac gatcatgcgc ttatgtcgtg ctgaaagcgc cgcatcattc attatcactg
      181 gaagaggtgg tggctttttt tagccgtaaa cgggtcgcaa aatataaata tcctgaacat
      241 atcgtggtaa tcgaaaaact accgcgcact gcctcaggta aaatacaaaa gtttttgtta
      301 agaaaagata ttatgcggcg attaacgcag gatgtctgtg aatagattga ataagtttca
      361 tcttcgggga tcacataaac ccggcgacaa aacgccgccg ggtatttatt ttatttcttc
      421 agttcagcca ggcttaacca ggtttgcacc acggtgtccg gattcagaga caggctatcg
      481 atcccctctt ccatcaacca tgcggcaaaa tcttcgtggt cggacggacc ctgaccgcaa
      541 atcccgacat atttgccctg tttcttcgcg gcacggatag ccatcgacag caatactttc
      601 accgcatcgt tgcgctcatc gaacagttca gacaccacgc cggagtcacg atccagaccg
      661 agcgccaact gcgtcatgtc gtttgagcca attgagaagc cgtcgaaata ttcgaggaac
      721 tgctcggcca gcaaggcgtt ggacgggatt tcacacatca tgatgatttt cagcccgttc
      781 tcaccacgct tcagcccctg acgcgccagt tcttcaacca ccgctttcgc ctggtctacg
      841 gtacgcacga acgggatcat gatctcaacg ttggtcagcc ccatgtcgtt gcgcacacgt
      901 ttcactgctt cacactccag cgcgaagcag tcgcggaagc tgtcggaaac atagcggccc
      961 gcgccacgga agccgagcat cgggttctct tcatctggct cgtaacgctc accaccgacc
     1021 aggttggcat attcgttcga tttaaaatca gagagacgga caatgacgcg cttcggataa
     1081 aacgcggcac ccagcgtcgc gatcccttca gtcagacgac caacgtaaaa ttcacgcgga
     1141 gaatcaaaac ctttcatcat ctcgcggatt tcgttttgca actgcggttc ctgatcgtca
     1201 aactcaagca gtgcgcgtgg gtggacgcca atcatacggt tgatgataaa ttccagacgc
     1261 gcaagtccca cgccttcgtt cggcagacag gcgaagtcga aagctcgatc cgggttaccg
     1321 acgttcatca tcactttcaa cggcagatcc ggcatggttt ctacgctgga gcttttcacg
     1381 ctaaattcca gcaactccgc atagacgtaa ccggtatcac cttcggcaca agaaacagtg
     1441 acgttctcac cgtctttcat ccgttctgtt gcatcaccac agcccactac cgccggaata
     1501 cccagttcac gagcgatgat cgccgcgtga caggtacgac cgccacggtt ggtgacgatg
     1561 gcagatgctt tcttcatgat cggttcccag tccgggtcgg tcatgtcagt gaccagcacg
     1621 tcaccaggtt cgatgcggtt catttcgctg atgtcatgga tgactttcac cggacccgca
     1681 ccgatgcgat gaccgatagc acggccttcg gcgataatct taccctgtga atgcagcgta
     1741 taacgctcca tgacctgacc gcgtgagcgc acggtttccg gacgcgcctg cacaatgaac
     1801 agtttgccgg tgtggccatc tttcgcccac tcaatatcca tcgggcgacc gtagtgtttc
     1861 tcaatttgta cggcctgttt tgccagttcc tgcacttctt cgttggtcag cgagaagatg
     1921 tcacgctgtt cctgcggtac gtcttcgatt ttaacctgct tgccgtgctc ctgggtcggc
     1981 gcgtaaacca tgcggatttt tttcgacccc atggtgcggc gcacgatagc cgggcgattc
     2041 gccgccagtg tcggtttatg cacgtaaaac tcatccgggt taaccgcccc ctgcacgacc
     2101 atctcaccaa ggccccatgc ggaagtgata aacaccacct ggtcaaagcc ggattcggta
     2161 tcaatggaga acatcacgcc agaagatgcg aggtcggagc gcaccatccg ctgaacaccg
     2221 gcggagagcg ccacgccacg gtgatcgtaa ccctggtgca cacgataaga gatggcgcga
     2281 tcgttaaaca gagaagcaaa tacatgtttc actgccacga gaacggcgtc aaacccctga
     2341 acgttgagga aggtttcctg ctgaccggca aaggaagcgt ccggcatatc ttctgcggtg
     2401 gcggaggagc gcaccgcaaa agaggcgttt tcgtcatcgg cggaaagctg agcataggct
     2461 tcgcggatgg cgttttccag ctcaggctgg aagggagtgt cgataatcca ctggcggatt
     2521 tgcgcgcccg ctttcgcaag ctgagtaaca tcgtcaatat ccgttttatc cagcagttca
     2581 taaatgcgct ggtttacgtc gctttggtcc agaaactggt taaacgcgtc ggcggttgtg
     2641 gcgaaaccat tcggaacgga aacacccatt ccggaaaggt tagtaatcat ttcacccagg
     2701 gaggcatttt tgcccccaac cctgtctaca tcattcatgc cgagttggtt gtaccaaagc
     2761 accagcggtg acgagccatt gttggacatc gaacaatcct tttgtgataa atgaacggtt
     2821 tgagaaacac atttctgcgc atttatcttt gcatatttaa ccggatgaaa aaaacgatga
     2881 atcgttcaag caaatatatt tttttacttt ttaagactga tc
//
LOCUS       DAJTNA010000300         2906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTNA010000300 DAJTNA010000000
VERSION     DAJTNA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.306"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(317..1126)
                     /locus_tag="OO161_004080"
     CDS             complement(317..1126)
                     /locus_tag="OO161_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCR5464917.1"
                     /translation="MIHLVNKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRS
                     SVYSSIDNLYKNGYIFLVDGETKEYEAKSPELILSQIEKSTIDNIKILKKELSQMMFQ
                     NEREFIYNVSGFDNLHQKAKEMLSSAEYEIYLNTDFDLHFLKNEICEALNRHVRVIVF
                     SFNKLITPDPRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSNNRLL
                     INIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNC"
     gene            complement(1532..2653)
                     /gene="ugpC"
                     /locus_tag="OO161_004081"
     CDS             complement(1532..2653)
                     /gene="ugpC"
                     /locus_tag="OO161_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR5464918.1"
                     /translation="MAAVILRKVEKIYDNGFKPVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMTFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRVAVGRAIVRKSDVFLFDEPL
                     SNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRIMQ
                     VDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAQNIRLYIPENKQSV
                     LKAYIDKPVCFGIRPEHISLSVNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFIAK
                     VNDQSITTVDIGKNLYFNLDTSFCHIFDFYTEENITNYL"
     gene            complement(2736..>2906)
                     /locus_tag="OO161_004082"
     CDS             complement(2736..>2906)
                     /locus_tag="OO161_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HCR5464919.1"
                     /translation="SHYPGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPD
                     KKAIAAMVAPCK"
BASE COUNT          913 a          542 c          498 g          953 t
ORIGIN      
        1 tatcccacca ataatattgc atcatgtgat catcgggatt attctgtttt ttgataacgc
       61 tatcttcagc agaaacagtg agtggagtta tacgatctct tccatatatt ttatgctccg
      121 taataagatg gggaactaat ttatccagtt ctcctatatg accgttcata taccattcca
      181 tcttattctt taaccaacca gtgggtttta tatcgttaaa aaaatcataa atataccttt
      241 gcataaaaaa gtttagacgt tataaaagac aaatagattg tgatcgccta cagacgatca
      301 caatgaaagg aaaatattaa cagtttttgc tatcactcaa atgtttttca tcctgcacca
      361 tatcattgcc agattcatgc aaagtattta ttaatgcatt ggcatcagga tattgttgct
      421 gatacttggt tagatatata tcactatgga tatgttcggc aattatattg ataagcaagc
      481 gattattcga gtataacccc agtgattcag ctcgttgtga aaaaaccatt actgtcttca
      541 tatcaacaac aagcataaaa cgatgagaag ggtaatattc ttcttcatgg ttaaaacggt
      601 aatatagttc tacgcgtggg tcaggagtaa taagtttatt aaatgaaaaa acaataactc
      661 gaacatgtcg atttagagcc tcacaaattt catttttcaa aaaatgtaaa tcaaaatccg
      721 tgttgaggta aatttcatac tctgcagaag agagcatctc tttagctttc tgatgtaaat
      781 tatcaaaacc agagacatta taaatgaatt cacgttcatt ctgaaacatc atctgtgata
      841 attctttttt caagattttt atgttgtcaa tagtgctttt ttcaatttgc gaaagtatga
      901 gttcaggaga ttttgcttca tattctttgg tttcaccatc gacaagaaag atataaccgt
      961 tcttgtaaag attatcaatt gatgagtata cagacgaacg tgaaagtgaa atttcctttg
     1021 caatcttgta accactgcta cgaccgtttt tcaaaagact gatgtaaacc tgtgcatcgg
     1081 tacgagtgaa accaaaattt atcaatttat ttaccaaatg tatcatagac gcgctcctga
     1141 actgaaaatg aaatcttacc aattttaaca ttattaatta acattttcga aaaattgctg
     1201 agataaaatg tgcatttatc gtcattcatc ctcaagctgg ctacaggtct acagcacata
     1261 agcattgtga actgtgaatc tgaatgccct ccaactatgg tcagaagaca tgctctgacc
     1321 taattagtag actcactgag taagcggtgt tttcaacggt ttattcatat atgctcttct
     1381 ttattgatgg aattttgtaa ctcaattacc tggataagaa tcatttcgtg aagttaatta
     1441 cattaccaat agacagttcg agcagtaaga agccttatgg aatggtaagg gtttaatacc
     1501 ggtattcaac ttggggttat tatcatatca atcacaaata attagttata ttttcttctg
     1561 tgtaaaaatc aaatatatga cagaagctcg tatcaaggtt gaaatacaaa ttttttccaa
     1621 tatcgactgt agtaattgat tgatcgttga cttttgcaat aaactctttg ttgttaacgg
     1681 taaagtaaag atatttttca ctccccatat tttcaataat acttaatgca ccagtgactg
     1741 tattcatttc tttgcaattc acactcaaag agatatgttc tggacgaata ccaaaacaaa
     1801 cgggtttatc tatatacgct ttgagtactg attgtttatt ctcaggtatg tacaaccgaa
     1861 tattttgggc aatgtcaata tagagtttac cgtcttcctc gcgtacaacc gcatctatca
     1921 aattcattga cggggaacca ataaagccag caacgaatct gttagcaggg tggttataca
     1981 gattaactgg tgtgtcgacc tgcattattc gcccctgatt aagcacacaa atacgatcac
     2041 cgagggtcaa tgcttcaatt tgatcgtgtg taacataaat catggttgct ggatgaccct
     2101 catccttgag agactgatgc aactgagcaa ttttgactcg cattgaaaca cgaagtttcg
     2161 catcaaggtt agatagtggc tcatcaaata agaaaacatc tgacttacgg acaattgcac
     2221 ggcctaccgc gacacgctga ccacccgaca tttctttcgg ttttcggtaa agtaaatcgc
     2281 taatttcaag tttttctgca acttcttcga caattttctt aatctcttct tttggccttt
     2341 tttgcatttt taaaccaaaa gtcatattgt caaatacagt cttatgggga tacagcgcat
     2401 agttctggaa caccattgca atgctgcgat ctttaggcgg aagggtatta acaaggcggt
     2461 cgctaatgta aatttcacca cctgatacgt cttcaagacc tgcaatcatg cgtaatgtag
     2521 ttgatttggc acacccagat ggtccaacaa aaaccatgaa ttcaccatcg tttatatcga
     2581 gatcaatgcc gtgaacaggt ttgaatccat tgtcataaat tttttcaact ttgcgtagaa
     2641 ttaccgcggc cataatctgc tctcattaac aagaattttg gaatactgat actccactat
     2701 tcaacgaatg ttgaatagtg gagtgctaaa aataactatt tacatggtgc taccatggcg
     2761 gctattgcct ttttatcagg accaatttga atcgtccaaa catactttcc atcggcagga
     2821 atgacgactt ttgtattttt atccatgcct cctttcttga gtgttttagc ctgatttaat
     2881 ggcaattcaa gacctgggta atgact
//
LOCUS       DAJTNA010000301         2882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTNA010000301 DAJTNA010000000
VERSION     DAJTNA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.307"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(40..261)
                     /locus_tag="OO161_004083"
                     /pseudo
     CDS             complement(40..261)
                     /locus_tag="OO161_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(258..521)
                     /locus_tag="OO161_004084"
     CDS             complement(258..521)
                     /locus_tag="OO161_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acetylcholine receptor subunit beta"
                     /protein_id="HCR5464920.1"
                     /translation="MNINLIYRHPCELEIESLLGREEPYPDTFTPADCATERLTRART
                     GLVHVMNEIVPSVGGEQATVINSWLQKVTSLIDIGLIDVESAK"
     gene            complement(518..712)
                     /locus_tag="OO161_004085"
     CDS             complement(518..712)
                     /locus_tag="OO161_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464921.1"
                     /translation="MNNSINTPRLTSALQLIEQAAAVLVAVSLSAEEMDATDVVDAIK
                     ACSSLVNDARAELVILGGEK"
     gene            complement(705..1772)
                     /locus_tag="OO161_004086"
     CDS             complement(705..1772)
                     /locus_tag="OO161_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5464922.1"
                     /translation="MIPVQKKTFSLAGMSPKSRNMTAKSGINTADTSKVYHLLVVGTD
                     ALTIPEISGDDISIEKVSGCARKFLVVDDVFSSCSDSTKTFVHARDVNEMSAMYCASG
                     SSNSEFSESIKKSLPLCGNTVYGYKAPHKTGAGIGVLVNRMATYDAPSVFFYVVGLTH
                     PFFGRWCIIQRLCQSMVAQAGASSEAPVSIRAGYANPVWATTSEIGVSGGSVTCYRME
                     AATCWLLPLPKNRNLSGLSPQFAAIARQLPPKFIILLPSLNAMLAVLWCAITSAFLLV
                     VSAWRWHMIKTYDVHMDPLERTSQIITLTEVINDILVSNSPSRDERLKALLAILDLAV
                     RDVHFLLEGGEMPGKTGATNE"
     gene            complement(1769..1948)
                     /locus_tag="OO161_004087"
     CDS             complement(1769..1948)
                     /locus_tag="OO161_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5464923.1"
                     /translation="MSKPTGTPQPQKRYKDAHGALVTVESVSHNRVTFYRDGYQSPCV
                     QPLARFMKEFAEVNQ"
     gene            complement(1941..2774)
                     /locus_tag="OO161_004088"
     CDS             complement(1941..2774)
                     /locus_tag="OO161_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR5464924.1"
                     /translation="MNIEKSRLISEAAPHLNASLSTINGNEFAAIVPVIPGHIGGRET
                     NIVSAKALHKALGVGKDFSTWITDRISEYDFTIGHDYSVHKTISPNLGKSPNGAAYSK
                     IKQSGRPGKDYLLSVGMAKELAMIERNDQGHAIRRYFIQCEEELQRSVPEIAARYRRQ
                     LKARISAANNFKPMCDALNMARAEMGKTTQQHHYTNESNMISRIVLGGLTAKQWARIN
                     GYSGEPRDHMNAEQLEHLSYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEALN
                     V"
BASE COUNT          679 a          721 c          701 g          781 t
ORIGIN      
        1 acgccgtcag gcgttgcttc tgcatggaaa gttaagaaat tattcatgtt tcgcctcgcc
       61 tgaatgattc tgtaactcat atttgcccaa caattcgtcc gcttcctaaa tagcttctgg
      121 tacgccattg agcagtgaca taaccgcgcc aatcattaac ttgtctctgt tgctggggct
      181 ggccccttca agccagaacc ctaaaacagt ctgaacctgt tcgatgcgac attgcgcttc
      241 aatgagctgg atgttggtca tttcgcactc tccacatcga ttaaaccgat atctatcagg
      301 gaggtaactt tttgtagcca gctattgatt actgtcgcct gttcccctcc caccgaggga
      361 acaatctcat tcatcacatg aaccagacct gtgcgcgctc tggtaagccg ttcagtcgcg
      421 caatctgcgg gagtgaatgt gtctggatat ggctcttcac gccccagcaa tgattcaatt
      481 tccagctcac acggatgacg ataaattaag ttgatattca tttttcaccc ccaagaatta
      541 ccagctcggc acgggcatcg ttaaccaaag atgagcacgc tttaatcgca tccacgacat
      601 cagtagcgtc catttcttca gccgaaagac tgacagcaac caggacagcc gctgcttgct
      661 cgattaattg aagtgcggac gtaaggcgag gggtattaat tgagttattc attggttgcc
      721 cccgtttttc ctggcatttc gccaccttcc agcaggaaat gaacgtcacg aacggcgaga
      781 tccaatatcg cgagtaacgc cttcagtctt tcgtctcgtg agggagagtt gctcaccaga
      841 atgtcgttaa tcacttctgt cagtgtgatg atctggcttg tgcgttcgag gggatccata
      901 tgcacatcgt aggttttaat catgtgccac ctccatgcgg atacgaccag caaaaaagca
      961 gatgtgatcg cgcaccagag aacggcgagc atcgcgttca gactcggcag caatatgatg
     1021 aattttggcg gtaattgtcg ggcaatcgcg gcgaactgcg gcgataatcc agataaattg
     1081 cggtttttgg gtaggggtag tagccagcat gtggcagcct ccattcgata gcatgtaacg
     1141 ctaccaccgg aaacgccaat ttcgctggtg gtagcccaga cagggttggc gtaaccggct
     1201 cgaatggata ccggcgcttc cgaagaagcc cctgcctgag ccaccattga ttgacaaagg
     1261 cgctggatta tacaccaacg cccaaaaaat gggtgagtca gacctacgac ataaaaaaag
     1321 acgctcggcg cgtcatatgt cgccattcga tttaccagga cgccaatccc ggcacccgtt
     1381 ttatgaggtg ccttataacc ataaaccgtg ttgccgcata acggcaagct cttttttatg
     1441 gactccgaaa actcactatt ggaagaacca gaagcacagt acatcgcgct catttcgtta
     1501 acgtcacgcg cgtggacgaa tgtttttgtg gagtcactac acgagctgaa gacatcatct
     1561 acgaccagaa attttctggc gcagccgctg accttctcaa tgctaatatc atcgcccgag
     1621 atttctggta tggttaaggc atccgttccg accacaagca aatgataaac tttgcttgta
     1681 tctgctgtat taatgcctga ctttgccgtc atatttctgg attttggcga catgccagcc
     1741 agtgaaaatg tcttcttctg gacgggtatc attggttaac ctccgcgaac tccttcatga
     1801 aacgcgccag cggttgtacg catggcgatt gatacccgtc gcgataaaac gtcactcggt
     1861 tgtgagacac gctttcgaca gtaacgagtg ctccgtgggc atctttgtag cgtttttgtg
     1921 gttgtggtgt tcctgttggc ttagacattg agcgcctcca gacgcttggc gagccagcgt
     1981 tgcgacaggc gggtgagttc tcctttgcgc tgctcatatt ccatgcccat atcaattaac
     2041 gtgatattgg tgctttcgag atatgagagg tgctcaagct gttctgcgtt catatggtcg
     2101 cgaggttcgc cagaatagcc atttatccgc gcccactgct tagcagttag cccaccaaga
     2161 acgatacgag aaatcatatt gctctcgttt gtgtagtggt gttgctgcgt cgttttcccc
     2221 atctcggcac gggccatatt cagcgcatcg cacattggct taaagttgtt tgcggcactg
     2281 atacgggctt ttagctggcg acgatagcgt gcggcgattt caggcacgct acgctgtaat
     2341 tcttcctcgc actggatgaa ataacggcgg atagcgtgac cctgatcatt gcgttcgatc
     2401 attgccagtt ctttcgccat tccgacactt aacagatagt ctttgccggg tctgccagac
     2461 tgcttaatct tgctgtaagc cgcgccattc gggcttttcc ccaaatttgg ggaaattgtt
     2521 ttatggactg agtaatcgtg cccaatggtg aagtcatatt cagagatgcg atcagtgatc
     2581 catgtagaga agtcttttcc cacgcccaac gctttgtgta acgcttttgc gctaacaata
     2641 ttggtttcac gcccaccgat atgaccagga ataaccggga caattgcggc aaattcatta
     2701 ccgttaattg tgctcagaga ggcgttcaga tgaggggcgg cctcagaaat taatctgctt
     2761 ttttcgatgt tcattttcag tctccgttaa gcggcgttaa acgcgtccgg gtagagtttc
     2821 agaatgtcgg caatttcctg ctgagaaagt ccgttgtgat catttatgtt cgcaagatgg
     2881 tt
//
LOCUS       DAJTNA010000302         2872 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTNA010000302 DAJTNA010000000
VERSION     DAJTNA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.308"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..567)
                     /gene="ospC2"
                     /locus_tag="OO161_004089"
     CDS             complement(<1..567)
                     /gene="ospC2"
                     /locus_tag="OO161_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5464925.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFF"
     gene            992..1836
                     /locus_tag="OO161_004090"
     CDS             join(992..1334,1334..1836)
                     /locus_tag="OO161_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR5464926.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFIDWSATALD
                     GSNIRALKCAAGGSKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAH
                     ESQFAQRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNE
                     KMASDGRAQLDRDAYRNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFY
                     QRLRN"
     gene            complement(1985..>2872)
                     /gene="ospC2"
                     /locus_tag="OO161_004091"
     CDS             complement(1985..>2872)
                     /gene="ospC2"
                     /locus_tag="OO161_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5464927.1"
                     /translation="GVEISNHQETLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHF
                     DNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLID
                     FIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLE
                     DAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYILSKFNFTKQDVAEMEKMN
                     NNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKANSGDTMLDNAMKGKDS
                     KTIDFLLKNGAVSGKRFGR"
BASE COUNT          856 a          569 c          551 g          896 t
ORIGIN      
        1 aaaaaaaaca aaatcgtggt tagctatttc ttcaatatcc tcagagaatg tgtttttata
       61 tgtttgtata ttctcagact taattttatc aaaggactta atattaagag tattatttga
      121 caggttagcg ttactttgat gtctgaagtt aaaatcagaa ttaataataa tagagagcaa
      181 gtgacgttca tccgaagaaa gaggatgagt tttttgtatt atttcagaaa gatagtgtct
      241 ggacacctta tcgaggtgct caagtgaccg aaatgatgac cgtgctccag atggcgcttc
      301 aatagaaaga tttaatgatt tgaaactggt cccttgagag aacattttac tataactttg
      361 cgctgctata gacttcctta aaaagctttt cacagtatca gcacagtgat ctaaatgttt
      421 ttcacttatg ccagaagagg atgagtttgt tacgcatgat atgttttttt gtaatgcttt
      481 tgtatctttg ttcggattta tttttccatc aacaagatca atattgttct gaacattaat
      541 atgatttact gcttcaggta ttttcataaa acatccccca ttatgtgcgt ttttatctgc
      601 agttatttag aataatatta tctaacttaa tattttcttg tatgtattat aacgaacata
      661 ttttaatatg ttgtacgatt ttcactctga aatcccctga gtttaatcac acttccttta
      721 ctgaaaggaa ttattgcaac agtattattt gtttttggat atatatcttg cgccttgtta
      781 tgtagttaat gctcagaata tgatagataa aatagagacg cagaacaggg catgctcata
      841 acatgatgat aacataaaaa attagtaaca ataaatgaaa gaatcatggg cgctcaacaa
      901 aaagagagct aaatagctgc acggaatagt aggctgtgtc ccttaatcat ctgagttata
      961 gtaactgtct gtttacacaa catattgaac tatggctcgc tacgaccttc ccgatgaggc
     1021 atggaccatc atcaagcccc tgttacctcc tgaacccgcc acaccacggg ccggacgccc
     1081 atgggctgag caccgtaaaa tcatcaatgg catgttctgg gtgttatgtt ccggtgcacc
     1141 atggcgcgat ttacccgaac gatatggggc atggaaaact gtttataacc gctttaaccg
     1201 atggtcaaag tcaggcgtga ttaatattat tttcaacagg ttgctttcgc tacttgatgc
     1261 aaacggcttt attgactggt ctgccaccgc gctggatggc agtaatatcc gggcgctgaa
     1321 atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg gagataatgg cccgggtcgc
     1381 tctcgcggcg gttttggcac caaaatccat ctggcaacag acggaagtgg cctcccgtta
     1441 aatatcgtgc taagccccgg acaagctcat gaaagccagt tcgcgcaacg acttctggac
     1501 gggattggcg ttcagcgtca gaacggcagc atgaaacgcc gtggacatgc ggtactggct
     1561 gataaagcgt attcagggcg cgcattgcgc aacgagctga aaaataacgg tataaaggca
     1621 gtaatccccc gaaagtcaaa tgagaaaatg gcatcggatg gacgtgcaca gcttgatcgt
     1681 gatgcgtacc gcaatcgtaa tgtcgttgaa cggtgctttg ggcggctgaa agaatatcgc
     1741 cgcatcgcca cgcgttacga caaaacggcg agaaattacc tggcgatggt gaaactgggc
     1801 tgcatccgac tcttttatca acgcttacgt aattaaggga cacagcctag tctccaggat
     1861 tcccggggcg ttcaccacat gcaatgtgtt gttgcagggg aacggtctgc accatcccct
     1921 atatcgatca atataacaaa aatgacaacg tcagattcag ggcacaacct aaatatattt
     1981 taggtcacct cccaaatcgt ttgcctgata ccgctccatt ttttaataaa aaatcaattg
     2041 tttttgaatc tttgcctttc attgcattgt caagcatagt gtccccacta tttgcttttt
     2101 gaaatctttt gtttacatca accaatccat tattaataaa atattctagc actttatagt
     2161 tggcactatc ttcactaagt aaatactcaa catcatacag ctcacaatac atattgttgt
     2221 tcattttctc catctctgcg acatcttgtt ttgtaaaatt aaatttagat agtatataaa
     2281 gtgccatatc agattttgca ttgcctagag aataagcaag agcttgatga gccatttttt
     2341 tatcagtcac cttattgtta atttcttcat aattggatgc tttaatggca tcctccaaag
     2401 atatatctct gagtcttatc tttttgaagt tatctgtgct tagcattctt ggtatatgat
     2461 actcaagctg aaacacaaag tttataattc tatccagact aacagggtca atgtttttat
     2521 tataacaaaa ctctcggaat ctttgatctt tactctttct tataaaatca ataagatgca
     2581 acccaattcc aagcctcata tctttagtat taaatattgg cacatcagtt tttccctgat
     2641 taccatgcac atacctgcta acttcatcca caacctcttt aaagttatct aagaaaaatg
     2701 attggtgctc atggtaaaaa acaggtggaa tagcattatc aaagtggtcg gttaatgtca
     2761 tatatccata tggagagtca tgatcaatga tatacgcatt agcaccaaaa tccacagtat
     2821 gatgtgtttt atttagtggg agtgtttctt gatgattgga aatttcaacc cc
//
LOCUS       DAJTNA010000303         2866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTNA010000303 DAJTNA010000000
VERSION     DAJTNA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.309"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            60..389
                     /locus_tag="OO161_004092"
     CDS             60..389
                     /locus_tag="OO161_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5464928.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            389..1087
                     /locus_tag="OO161_004093"
     CDS             389..1087
                     /locus_tag="OO161_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5464929.1"
                     /translation="MQDIREESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNEPNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            1098..1841
                     /locus_tag="OO161_004094"
     CDS             1098..1841
                     /locus_tag="OO161_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5464930.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQPGDILLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            1739..2419
                     /locus_tag="OO161_004095"
     CDS             1739..2419
                     /locus_tag="OO161_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5464931.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNSAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            2660..2866
                     /locus_tag="OO161_004096"
                     /pseudo
     CDS             2660..2866
                     /locus_tag="OO161_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          618 a          686 c          953 g          609 t
ORIGIN      
        1 gcacaggatg aactccggct gcagttgcgt gatggcggta tgttatcagg gagcggtgga
       61 tgaaaacctt tcgctggaaa gtgaagccgg atatggaggt gaactcgcag ccatcggtgc
      121 gtgaagtgcg ttttggtgac gggtactcac agcgtatggc ggcagggctg aatgctgacc
      181 tgaaaacata cagggtgacg ctttccgtga cccgggagga ggcccggcat ctggaagcgt
      241 tcctggcaga gcacggtggc tggaaggcat ttttgtggaa gccaccctat gcataccggc
      301 agataaaggt gacctgtgcc gggtggtctg cgcgggtcgg gatgttgcgc gttgagttca
      361 gcgcggagtt taagcaggtg gtgaactgat gcaggatatt cgcgaagaaa gtctgagcga
      421 gtcggttaaa tcagagcagt caccgcgggt ggtactctgg gaaatcgacc tgacggtaca
      481 gggcggtgag cggtattttt tctgtaatga gccgaatgaa aaaggggagc cggtcacctg
      541 gcaggggcgt aagtatgagg catatccgat tgacggcagc ggctttgaga tgaacggccg
      601 gggcagcagt gccagaccgt cgctgacggt gtccaatctg ttcggtctgg tcaccgggat
      661 ggcggaagac ctgcagagtc tggtgggggc cacggtggtc cgccgccggg tgtatgcccg
      721 ttttctggat gcggtgaatt tcgttgcggg caatccggag gcggacccgg agcaggagct
      781 gagtgaccgc tgggtggtgg agcagatgtc gcagctgaca gccatgacgg cctcgtttgt
      841 gctggctaca ccgaccgaga cggacggggc gctgtttccc ggtcgtatca tgctggcgaa
      901 cacctgtatg tggacctacc gctctgatga gtgtggttac acgggcgggg ctgtggcgga
      961 tgagttcgat aaacccacca cggatatccg taaggacaga tgcagcaagt gcatgcgcgg
     1021 gtgtgaactg cgcaggaatg tcggcaattt tggcggtttc ctttccatta ataaactttc
     1081 gcagtaaatc ccggtttatg acacagactg aatcagcgat tctggcgcat gcccggcggt
     1141 gtgcgcctgc ggagtcgtgc ggcttcgtga taagcacgcc ggagggggag tggtatatcc
     1201 cttgtgtgaa tatttctgca gagccggagg cgtattttcg tatcgcaccg gaagactggc
     1261 tgcgggcaga gatgcagggg gagattgtgg cactggtcca cagtcatccc ggtgagctgc
     1321 cctggctgag cgaggctgac cggcggctgc agataaaaag cgcactgccc tggtggctgg
     1381 tctgccgggg ggaaattcat aaattccgct gtgtgccaca tctgacagga cggcgctttg
     1441 agcacggggt gacggactgt tacacgctgt tccgggatgc ataccatctg gcgggaattg
     1501 atatgccgga ttttcatcgc gaggatgact ggtggcgcaa cggccagaac ctgtacctgg
     1561 acaatatggc ggtcaccggc ttttaccggg tgcccctgtc ctctgcacag ccgggcgata
     1621 ttctgctgtg ctgctttggt gcttcggtac cgaaccatgc caccatttac tgcggcaacg
     1681 gtgagctgct tcaccatctg cctgaacaac tgagtaaacg ggagaggtat tccgaaaaat
     1741 ggcaacgacg aacgcattct gtctggcgtc accgccactg gcacgcatct gccttcacgg
     1801 ggatttgcaa cgatttggcc gccgcctcag cctgtatgtg aactcggcag cggaagccat
     1861 tcgcgccctg tcgatgcaga tgccgggctt tcgccgtcag atgaacgaag gctggtacca
     1921 gatacgtatt gccggtgatg acacggcacc ggaggcggtg tatgcccgtc ttcacgaaca
     1981 gctgggtgag ggaacggtca tccacattgt gccgcgactg gccggggccg gaaagggtgg
     2041 actgcagatt gtgttggggg cggcagccat cgtgggctct ttcttcactg ccggggcatc
     2101 aatggcgtta tggggttcag ccctggcagc cggtggtttt tctgccacca cgatgctgtt
     2161 ttcacttgga gccagcatga ttctgggcgg tgtggcccag atgctggccc cgaaggcaaa
     2221 aacaccggat taccgcgcaa cggataacgg cagacagaac acgtactttt cctcgctgga
     2281 taacatgatt gcccagggga acccgatgcc ggtgccttac ggggaaatgc tggttggctc
     2341 ccgccgtata tcccaggaca tcagcacccg tgatgaaggc gggggcggaa aggtcgtggt
     2401 tatcgggcga cagggataaa acataaaaaa atcccgcagt gatcgcggag ctgcggggac
     2461 agacaaatga agatcaatgt gaaggagttg tttttgttac tcgggcaaaa aaacactaac
     2521 gcagcgaaat tataagcgcc acagtcagtg tgtgaaaatg tgaagatatt cagaaatttt
     2581 attccgtcat gacgcaggca cccggtgagg tgcctgttgt ttttgtgagt gaacaattat
     2641 cacggtaaga ggtgatgtaa tgggcaaagg tggcggcagg gcgcacacac cgcgtgaggc
     2701 gaaagacaat ctcaaatcca cgcagatgat gagtgtgatt gatgcgattg gtgagggacc
     2761 gatagaaggc ccggtgaaag gcctgcagag tattctggtg aacaaaaacc cctctgacgg
     2821 acacggacgg taatcccgtg atacacggtg tgaccgcggt ctggcg
//
LOCUS       DAJTNA010000304         2860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTNA010000304 DAJTNA010000000
VERSION     DAJTNA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.310"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            173..1714
                     /gene="istA"
                     /locus_tag="OO161_004097"
     CDS             173..1714
                     /gene="istA"
                     /locus_tag="OO161_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HCR5464932.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRSHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            1729..2475
                     /gene="istB"
                     /locus_tag="OO161_004098"
     CDS             1729..2475
                     /gene="istB"
                     /locus_tag="OO161_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HCR5464933.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
     gene            2646..2834
                     /locus_tag="OO161_004099"
     CDS             2646..2834
                     /locus_tag="OO161_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464934.1"
                     /translation="MLTRKSIVPVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT          789 a          695 c          683 g          693 t
ORIGIN      
        1 atcactcaat aacttccatc gagatcgggt aataacattt gaacagatcg ctgaataaca
       61 tcgatggaga tcacttttga ctcattttgt tattcagtga tctccatcaa tgttattgga
      121 acttcacagg tgtgttgatc tgtatctttt gccattccgg taaaggatac ctatgccaac
      181 agttccaatt tctatgagaa aacttaaaga aattcttaga cttaaatacg gtgttggact
      241 cagccatcga caaattggtc gtagtcttgc aatctcccct tccgttgtat ccagatatgc
      301 taatcgggcg gctcaacttg gcataaagca gtggccctta cctacaggat gggatgatac
      361 aaaactaaaa catgcgttcc ttcagaccca ggttaagatg aagaagcact ctctgcctga
      421 ctgggctaca gtacaccggg aactgcgtaa taaatgcgtg acgctgcagc tactctggga
      481 agaatactgt gagcgtaatc caggcggttt ttacagctat aaccattact gccggatgta
      541 ccgtgaatgg ctcaaaacca cttcaccatc aatgcgtcag gtacataaag ctggcgaaaa
      601 acttttcgtt gattactgtg gacctaccgt tggcgttacc gaccctgaga ccggagaaat
      661 aagaactgct caggtcatcg tagctgttct cggggcatca agttacacat gggcagaggc
      721 cacctggtct cagcagcttg aagactgggt gatgagtcat gttcgctgct tccagtggtt
      781 gggtggcgtt cctgaacttg ttgttccgga caatctgaaa agcgccacat ccagggcatg
      841 taagtatgat cctgacgtta accctaccta ccagcagatg cttgagcatt ataatgtcgc
      901 agttttgcct gcgcggccac gtaaaccgaa agataaagcc aaagctgaag ttggcgttca
      961 ggttgttgaa cgctggatca tggcccgaat caggcatgag atcttctaca gccttgcatc
     1021 gcttaatcag cgcattcggg agttgctgga aagactgaat aacaaaataa tgcagaagtt
     1081 gggttattca cgtgcagaac tcttcatcca gcttgataaa cccgcactga agcctcttcc
     1141 tgaagccagt tacagttaca ccctggtgaa gaaagtcaga gttcatgccg attaccacgt
     1201 ggaaatcgac aaacattact actcggttcc atgttcgctg ttaggccagc aactggaagc
     1261 atggatctcc ggagaactgg taagactctt caatcagggg caggaggttg ctgtgcaccc
     1321 gcgcaagcgt acttatggct acagtacccg caacgagcac atgcctgaag ctcatcgaca
     1381 gcatgccacc tggacgccag agcgtcttct ggaatgggcg gggcacatag gcagtgaaac
     1441 tcatagttat gtgcttcata tactgaactc tcgttcacat ccggaacaaa gctatcgctt
     1501 ctgccttgga ctcctgaacc ttcataaaaa atacagtaaa gccagactta atgcagcatg
     1561 tgcaagagct ctgaaaacaa aggtatggcg tctgtcaggt attaaatcga tcctggaaaa
     1621 aggtctggat aaacaacctg ttcaggatcc aaaaccagat ctgttatcca cgatggaaca
     1681 cgaaaacgta cgcggcagtg agtattacca ctgatacggg atccaatgat gaatcatctt
     1741 tacgaacaac tgaccgcact taaactcacc ggcttccgtg atgcgcttaa aaagcaactt
     1801 gctcagccgg gcacatacca ggagctgggc ttcgaagaac gcctgtcatt actgacagca
     1861 gaagaactaa cctgccgtga aaacaggaag gcagagcgtc tgatcaaaca tgcacggttc
     1921 agacttaatg ctgagttatc aaagctggat tatcgtaaca atagagggct ggacagggcc
     1981 ctcatccgtt cactcagtca gggaaactgg ttaaccctga aacaaaatat tttactgacc
     2041 ggggccaccg gcagcggtaa aacgttcctg gcatgtgcac ttggtcataa tgcctgccga
     2101 cagggataca aggtctacta ttatcgcctt aaagcgctga tggaacagtg ctatcagggg
     2161 catgctgatg gaagatacag caaacttttg accaggctga ataatagcga tctgctgctt
     2221 ctggatgact gggggctgga acctctctca tcagaacagc gtagcgacct gctggaaata
     2281 gtggatctga tgtaccaacg aggctcaatc atcgtagtga gccagttgcc ggtggaaaac
     2341 tggtacaaaa tgatcggaga ctccacacat gcggatgcca tcctagatcg actggttcat
     2401 ggcagtatca agatcgaact taaaggagaa tcaatgcgga aaatacaatc tccgttgacc
     2461 gaaggagatc agtgaaggta atttaaaaac ggttctgtga aagtgacacg aaccgatctc
     2521 catcgatgtt actcaccgat ctccttcacg gtaatacgca ccgcccctgt tcgtcctgtt
     2581 gttgccgcac gctccgcaga atatgtatgc tctgaggacc tgttttatta aggactcatc
     2641 ctcttatgtt gacccgcaaa agtatagttc ccgttctgct ctctgttggt gctgagaagc
     2701 tctcacaacg tgaatgggac tggatgaaga tgctcaaacc catggaccca ccacctgcga
     2761 tggtcgcggc ctccatcctg gagcgccgtg gcgacacagc agcccttacc cggctacagg
     2821 ataccggggg ttaatccgtc ctcatgttgt ccgcttttac
//
LOCUS       DAJTNA010000305         2857 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTNA010000305 DAJTNA010000000
VERSION     DAJTNA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.311"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            84..950
                     /gene="ydiB"
                     /locus_tag="OO161_004100"
     CDS             84..950
                     /gene="ydiB"
                     /locus_tag="OO161_004100"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5464935.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            982..1740
                     /gene="aroD"
                     /locus_tag="OO161_004101"
     CDS             982..1740
                     /gene="aroD"
                     /locus_tag="OO161_004101"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5464936.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASALGQISVNDLRTVLTILHQA"
     gene            1886..>2857
                     /locus_tag="OO161_004102"
     CDS             1886..>2857
                     /locus_tag="OO161_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464937.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLLIISPTGLGNRAERGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGC"
BASE COUNT          787 a          646 c          725 g          699 t
ORIGIN      
        1 cctgagaatg atgtgcggtt tggcgagcgt aaatttagca cccggttaaa cacaattaag
       61 catagaggtt aaaggagtta attatggatg ttaccgcaaa atacgaattg attgggctga
      121 tggcctatcc tatccgccac agtttatcgc ctgaaatgca gaataaagcc ttagaaaaag
      181 cgggattgcc atttacctat atggcctttg aagtggataa cgatagtttt cccggagcaa
      241 ttgaaggatt aaaagctcta aaaatgcgcg gaactggtgt atcaatgccg aacaaacaac
      301 tggcttgtga atatgttgat gaattaacgc cagcagccaa actggtgggt gccatcaaca
      361 ccatcgttaa tgatgatggc tacctgcgcg gctataacac cgacggcacg ggccatattc
      421 gcgccattaa agagagcggt tttgatatca aaggcaaaac aatggtgctg ttaggggccg
      481 gaggtgcctc aacggcgatt ggcgcgcagg gggcaattga aggtttaaaa gaaattaaac
      541 tctttaaccg tcgggatgag ttcttcgata aagccctcgc cttcgcgcag cgggttaacg
      601 aaaacaccga ttgtgtcgtc acggttaccg atctcgccga tcagcaagcc tttgctgaag
      661 ccctagcttc cgccgacatt ttaaccaatg gcacaaaagt gggtatgaaa ccccttgaga
      721 atgaatcatt agttaatgat atcagtctgt tacatccggg acttctggtc actgaatgcg
      781 tgtataaccc gcatatgacg aagttattgc agcaggcgca acaagctggt tgcaaaacga
      841 ttgatggata cggcatgttg ttgtggcaag gggctgaaca gttcacgtta tggactggca
      901 aagatttccc tctggaatat gttaaacagg tcatggggtt cggtgcctga caggctgacc
      961 gcgtgcagaa agggtaaaaa aatgaaaacc gtaactgtaa aagatctcgt cattggtacg
     1021 ggcgcaccta aaatcatcgt ctcgctgatg gcgaaagata tcgcccgcgt gaaatccgaa
     1081 gctctcgcct atcgtgaagc ggactttgat attctggaat ggcgtgtgga ccactttgcc
     1141 gacctctcca atgtggagtc tgtcatggcg gcggcaaaaa ttctccgcga aaccatgcca
     1201 gaaaaaccgc tgctgtttac cttccgcagt gccaaagaag gcggcgagca ggcgatttcc
     1261 accgaggctt atattgctct caatcgtgca gccatcgaca gcggcctggt tgatatgata
     1321 gatctggagt tatttaccgg cgatgatcag gttaaagaaa ccgtcgcctg cgcccacgcg
     1381 catgatgtga aagttgtcat gtccaaccat gacttccata aaacgccgga agccgaagaa
     1441 atcattgccc gtctgcgcaa aatgcagtcc ttcgacgccg atattcctaa gattgcgctg
     1501 atgccgcaaa gtaccagcga tgtgctgacg ttgcttgccg cgaccctgga gatgcaggag
     1561 cagtatgccg atcgtccaat catcacaatg tcaatggcaa aaactggcgt aatttctcgt
     1621 ctggctggtg aagtatttgg ctcggcggca acttttggtg cggtaaaaaa agcctctgcg
     1681 ctagggcaaa tctcggtaaa tgatttacgc acagtattaa ctattttaca tcaggcataa
     1741 gcaataatat ttcggcggga atatcctccc ccgccgaact aaaaaaaata tattcaaccg
     1801 tatttaataa aaatatttcg tgagtctctg tgcgctaaat atccatctgg cgtagggaaa
     1861 atcacacctg aatcaggaat taacaatgaa acctgtaaaa ccacctcgta ttaatggacg
     1921 agtgccggtc ctgtcggcac aggaagcggt gaattatatt cccgacgaag caacactttg
     1981 tgtgttaggc gctggcggcg gtattctgga agccacgacg ttaattactg ctcttgctga
     2041 taaatataaa cagactcaaa cgccacgtaa tttattgatt attagtccaa cagggcttgg
     2101 caatcgcgcc gagcgtggta ttagtccgct ggcgcaagaa ggtctggtga aatgggcatt
     2161 atgtggtcac tggggacaat cgccgcgtat ttctgaactc gcagaacaaa ataaaattat
     2221 tgcttataac tatccacaag gtgtacttac acaaacctta cgcgccgccg cagcccacca
     2281 gcctggtatt attagtgata ttggcatcgg gacatttgtc gatccacgcc agcaaggcgg
     2341 caaactgaat gaagtcacga aagaagacct gattaaactg gtcgagtttg ataacaaaga
     2401 atatctctat tacaaagcga ttgcgccaga tatcgccttc attcgcgcta ccacctgcga
     2461 cagtgaaggc tacgccactt ttgaagatga ggtgatgtat ctcgacgcat tggttattgc
     2521 ccaggcggtg cacaataacg gcggtattgt gatgatgcag gtgcagaaaa tggttaagaa
     2581 agccacgctg catcctaaat ctgtccgtat tccgggttat ctggtggata ttgtggtggt
     2641 cgatccggat caaagccaac tgtatggcgg tgcgccggtt aaccgcttta tttctggtga
     2701 cttcaccctt gatgacagca ccaaacttag cctgccccta aaccaacgta aattagttgc
     2761 gaggcgtgca ttattcgaaa tgcgtaaagg cgcggtgggg aatgtcggcg tcggtattgc
     2821 tgacggtatt ggcctggtcg cccgggaaga aggttgt
//
LOCUS       DAJTNA010000306         2825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTNA010000306 DAJTNA010000000
VERSION     DAJTNA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.312"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(44..412)
                     /locus_tag="OO161_004103"
     CDS             complement(44..412)
                     /locus_tag="OO161_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464938.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            932..1627
                     /gene="minC"
                     /locus_tag="OO161_004104"
     CDS             932..1627
                     /gene="minC"
                     /locus_tag="OO161_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5464939.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1651..2463
                     /gene="minD"
                     /locus_tag="OO161_004105"
     CDS             1651..2463
                     /gene="minD"
                     /locus_tag="OO161_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5464940.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2467..2733
                     /gene="minE"
                     /locus_tag="OO161_004106"
     CDS             2467..2733
                     /gene="minE"
                     /locus_tag="OO161_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5464941.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
BASE COUNT          806 a          643 c          672 g          704 t
ORIGIN      
        1 ttggcagcat taccgccgaa tagaacatta tgtaaacaag gcatcactca cgacacataa
       61 ataacatctt tgtggtgatt tgattgattt ttccactccg cttaccatcc acaccaaaat
      121 aacgatcatc acgacataat ttttctttaa catcgcaaaa tacaccgtta gcgaatgtaa
      181 attcgctggg atcgtagcct tgtaatgatt gtaagttttc agcggctttt tcgccgagat
      241 atttccttgt taactctggg gaaatgccat ctgagttcac acaaatatac gggtcgcaaa
      301 caacgccgca cgccggagat tgaagtccgc cagcgtcatg tccagacgcc attgatgaga
      361 acatcatccc cacacctgac aatccgatat aaaaaatacg catcatattc atagcacatc
      421 ctgataatcc aaccgtacaa cacttatgct catttcatca tcaatccgta atcaaaaccg
      481 ctgacctgga cgagttacga tgacctgaca gaagtatcag acgtcttctg cggtttctat
      541 gatagagcaa ttctgtggcg gatattaaga taacgggtat ctgttcttta atgatgctgc
      601 aaaaaatgga aagttaagaa agcctggcgt ggtgaaaaaa tacacttgcc tcaatactag
      661 atatttaaac aggtctggag atgctgtcac gattagggtg cggaaaaagg aagcgtaaat
      721 aagaacaatc aaaagtacga cggcaatggg ttgattgaca agggtatttt ttaagctatg
      781 aatcagcgcc atttatcaca gaatagactt ttactctgaa taaatgggag ggtgacttgc
      841 ctcaatataa tccagactat aacatgcctt atagtcttcg gaacatcatc gcgcgctggc
      901 gatgattaat agctaattga gtaaggccag gatgtcaaac acgccaatcg agcttaaagg
      961 cagtagcttc actttatctg tggttcatct gcatgaggca gaacctaagg ttatccatca
     1021 ggcgctggaa gacaaaatcg ctcaggcccc cgcattttta aaacatgccc ccgttgtact
     1081 caacgtcagt gcactggaag acccggtaaa ctggtcagcg atgcataagg cggtgtcggc
     1141 aaccggtttg cgggttattg gcgtaagtgg ctgcaaagat gcgcaactta aagccgaaat
     1201 tgaaaagatg gggctgccta tcctgacgga aggaaaggaa aaagcgccgc gtccagctcc
     1261 cgcaccacag gctccagcgc aaaatacaac gccggtcaca aaaacgcgtt taatagatac
     1321 cccggtgcgt tccggtcagc gtatttatgc tccacaatgt gatctgattg ttacaagcca
     1381 cgttagcgct ggggccgaat tgattgccga tgggaacatt catgtctatg gcatgatgcg
     1441 cggtcgtgcg cttgcagggg caagtggtga ccgggaaacg caaatatttt gtacgaacct
     1501 gatggcggaa ctggtgtcca tcgcaggtga atactggctg agtgatcaaa tcccagcaga
     1561 attttatggc aaagcggcgc gactgcagtt agtcgaaaac gctttgaccg ttcaaccgtt
     1621 aaattgatcc ctttttaaca aggaatttct atggcacgca ttattgttgt tacttcgggc
     1681 aaagggggtg ttggtaagac aacctccagc gcggccatcg ccactggttt ggcccagaag
     1741 ggaaagaaaa ctgtcgtgat agattttgat atcggcctgc gtaatctcga cctgattatg
     1801 ggttgtgaac gccgggtcgt ttacgatttc gtcaacgtca ttcagggcga tgcaacgcta
     1861 aatcaggcgt taattaaaga taagcgtact gaaaatctct atattcttcc ggcatcgcaa
     1921 acacgcgata aagatgccct cacccgtgaa ggggtcgcca aagttcttga tgatctgaaa
     1981 gcgatggatt ttgaatttat cgtttgtgac tccccggcag ggattgaaac cggtgcgtta
     2041 atggcactct attttgcaga cgaagccatt attaccacca acccggaagt ctcctcagta
     2101 cgcgactctg accgtatttt aggcattctg gcgtcgaaat cacgccgcgc agaaaatggc
     2161 gaagagccta ttaaagagca cctgctgtta acgcgctata acccaggccg cgtaagcaga
     2221 ggtgacatgc tgagcatgga agatgtgctg gagatcctgc gcatcaaact cgtcggcgtg
     2281 atcccagagg atcaatcagt attgcgcgcc tctaaccagg gtgaaccggt cattctcgac
     2341 attaacgccg atgcgggtaa agcctacgca gataccgtag aacgtctgtt gggagaagaa
     2401 cgtcctttcc gcttcattga agaagagaag aaaggcttcc tcaaacgctt gttcggagga
     2461 taagttatgg cattactcga tttctttctc tcgcggaaga aaaacacagc caacattgca
     2521 aaagaacggc tgcagattat tgttgctgaa cgccgtcgca gcgatgcaga accgcattat
     2581 ctgccgcagt tgcgtaaaga tattcttgag gtcatttgta aatacgtaca aattgatcct
     2641 gagatggtaa ccgtacagct tgagcaaaaa gatggcgata tttctattct tgagctgaac
     2701 gtgaccttac cggaagcaga agagctgaaa taagcccgct gtaaaaacgc atttatcttc
     2761 aaggcagagt catttctgcc ttgagttttt catccctctc atccacgttg tggtaaagcg
     2821 gcgag
//
LOCUS       DAJTNA010000307         2805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTNA010000307 DAJTNA010000000
VERSION     DAJTNA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.313"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            88..2603
                     /locus_tag="OO161_004107"
                     /pseudo
     CDS             88..2603
                     /locus_tag="OO161_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            2614..>2805
                     /locus_tag="OO161_004108"
     CDS             2614..>2805
                     /locus_tag="OO161_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5464942.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSST"
BASE COUNT          798 a          679 c          653 g          675 t
ORIGIN      
        1 ggctgcaatc aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca
       61 aatataaaaa acacaggtca tcagggaatg ccacaacgac accaccaggg acataaacgc
      121 acaccgaaac agttggcgct catcatcaaa cgctgtttgc cgatggtgct cactggcagc
      181 ggcatgcttt gcactaccgc taacgccgaa gagtattatt tcgaccccat tatgctggaa
      241 actacaaaaa gtggtatgca aacaactgat ctgtcacgtt tttcaaaaaa atacgcacaa
      301 ctaccaggaa cttatcaggt tgatatctgg ctgaataaaa agaaggtttc acagaaaaaa
      361 attacattta ccgccaatgc agagcaactt ctgcagccac agtttacggt agaacaacta
      421 cgtgagctgg gtattaaggt ggatgaaatc ccggcgctgg ctgaaaaaga tgacgatagc
      481 gtgatcaact cgcttgaaca aatcataccc ggtacagctg ctgaatttga tttcaatcat
      541 cagcgactta atttgagcat tccccaaatt gcactgtacc gtgatgcaag aggttacgtc
      601 tccccttctc gttgggacga tggtatacca acgctgttta ccaactactc gtttacaggt
      661 tctgataacc gttaccgcca gggcaatcgt agccaacgac agtacctgaa tatgcaaaat
      721 ggtgctaatt ttggcccctg gcgattacgt aactattcta cgtggacacg caacgatcag
      781 gcgtcaagct ggaacactat cagtagttat ttacaacgtg atatcaaggc gttgaagtct
      841 cagttgcttc tgggagaaag cgccaccagc ggcagtattt tttccagcta cacctttact
      901 ggcgtgcaac tcgcttccga cgataatatg ttgccaaaca gccagcgcgg atttgcccca
      961 acggtacgcg gtatcgcaaa cagtagtgca atcgtgacta tcaggcaaaa tggttatgtg
     1021 atctatcaaa gcaacgtgcc agcgggtgcc tttgaaatta acgatctcta cccctcttcc
     1081 aacagcggcg atttagaagt cacgattgaa gaaagtgacg gtacacaacg tcgctttatc
     1141 cagccttatt cttcattacc catgatgcag cgacctgggc atctaaaata tagcgcgacc
     1201 gctggacgct atcgcgctga tgcaaacagt gatagcaagg aacccgaatt tgctgaagcc
     1261 acggcaatat atggtttgaa taatactttt acgctgtatg gcggcctgct cggttctgaa
     1321 gattattatg cgctggggat cggtatcggc ggcacacttg gcgcactggg cgcgttgtcg
     1381 atggatatca acaggctgac acccaattcg ataaccagca ctcttttcat ggctatcaat
     1441 ggcgtacgca gtacatcaaa gatatcccgg aaaccaacac caatatcgct gttagctact
     1501 atcgctatac caacgatggc tattttagtt ttgatgaagc caatacccgc aattgggact
     1561 ataacagtcg ccaaaaaagt gaaattcaat tcaacatcag ccagacaata tttgatgggg
     1621 taagtctgta tgcctccggt tcgcagcaag actattgggg caataacgag aaaaacagga
     1681 atatctctgt tggggtttcc ggccagcaat ggggaattgg ttacagcctg aattatcaat
     1741 acagccgcta cactgatgaa aataatgacc gtgcactctc tttgaatctc agtattccgt
     1801 tagaacgctg gttaccgcgt agccgggttt cctatcagat gaccagccag aaagatcgcc
     1861 caacccaaca tgaaatgcgt cttgatggct cactgctgga tgatggtcgc ctgagctata
     1921 gcctggaaca aagtctggat gacgataaca accataacag tagcctgaac gccagttacc
     1981 gttcacctta tggaaccttc agtgccggat acagttacgg taatgacagc agccaataca
     2041 attacggcgt taccggcggc gtggttatcc atcctcatgg cgtgacgctc tcgcaatatc
     2101 tgggcaacgc ttttgcgctt atcgatgcta acggggcttc tggcgtgagg atacaaaact
     2161 atccggggat tgctactgat ccctttggct atgcagtgat tccttatctc acgacttatc
     2221 aggaaaaccg tctctcggta gatactacgc agctgcccga taacgtcgat cttgaacaaa
     2281 caacacagtt tgtggtgccc aacagaggtg caatggtagc ggcgcgtttc aacgccaata
     2341 tcggttatcg cgtacttgtt acagtcagcg atcgcaacgg taaaccgttg ccctttggcg
     2401 cacttgccag caacgatgat acggggcaac aaagtatcgt cgatgagggc ggcatactat
     2461 atctctctgg gatatcgagt aaatcacaaa gctggactgt acgctgggga aatcaggcag
     2521 atcaacaatg tcagtttgct tttagtacac cggattcaga accaacaacc tctgtattac
     2581 aaggcacagc gcagtgccat taaggataaa aaaatgaaaa gagcgcctct tataacagga
     2641 cttttgttga tatccacatc ctgcgcttat gcctcctcag gagggtgtgg agccgacagc
     2701 actagcggtg cgacaaatta cagcagtgtg gttgatgatg ttacggtgaa ccagacagat
     2761 aacgtgacag gacgggagtt tacctctgca acgctaagta gcact
//
LOCUS       DAJTNA010000308         2788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTNA010000308 DAJTNA010000000
VERSION     DAJTNA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.314"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..189)
                     /locus_tag="OO161_004109"
     CDS             complement(<1..189)
                     /locus_tag="OO161_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5464943.1"
                     /translation="MASISISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWRLQFT
                     YTASQPGTHQKIIDMAMNG"
     gene            479..1999
                     /locus_tag="OO161_004110"
     CDS             479..1999
                     /locus_tag="OO161_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR5464944.1"
                     /translation="MLLISIIQMFSVMIIMLNIKIKDAFRAIILLLLLLLSMVISIEI
                     SSIISIGEYIPTLALANISETNSVGWKIKLLPFLVFLSFMIASLILKRLIKEKKIRNI
                     MVVVFLLSLLIPISPINAFFSSSYEVYSEAKEYNMSPHEISDIKSKYTKETVTNDSYS
                     HEIINGNGKNILVIFIEGMSSLVISKELTPNLYEFRKKSISFNNYYNHTAATFRGLRG
                     QLTSSYQMTGGYHKDNSGLGQASKVEIEHKLNTYNYITKLPIILEENGYHTYFQASNS
                     IDDPLSLMLSTLKFNHIYGREDYNKKSGELTDKESFDLLFKKLNEAKQPFFYGVYTVG
                     THLGLDSPDVKFYDGRNEYLNHFHQLDHWFGDFISHFNKTSLSKDTIVIITSDHASYP
                     SEDYKKTFKTDNTFFVDQIPLIIWSNGVEFKDINAHGLNSLSLTPTILDILGIKESHN
                     MFIGESLFEKEKNKNELNCTSAIGDLYVKTCNGKMEIEKRKEKTELIKQLQLFGDR"
     gene            complement(2068..2788)
                     /locus_tag="OO161_004111"
                     /pseudo
     CDS             complement(2068..2788)
                     /locus_tag="OO161_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          922 a          504 c          508 g          854 t
ORIGIN      
        1 gccattcatg gccatatcaa tgattttctg gtgtgtacca ggttgagagg cggtgtaagt
       61 gaactgtagc cgccatgttt tacggcagtg agagcagaga tagcgctgat gtccggcagt
      121 acttttaccg ttacgcacaa cgccgtcagt agcggaacag gagggacagc tgatggaaat
      181 ggaagccacc ggagcacctc aaaagcaccg tcatccacta aatcagtaag ttggaagcat
      241 caccgcacta gcagacagtt aattaaaaaa gtataatccc tctccggatt tattgaataa
      301 tggagaatta tgctgttttc tatgtcttat ttagtcaagt gcgcttatca atattgtgat
      361 aagctcccga gtctaaactt aacttactga ttttaggttg aggaagaccg gcatttttgt
      421 agatccacgg cataatatct atttattcaa caacgccccc aaaaaaaggt ttttataaat
      481 gcttttaatt tcaatcattc aaatgttctc ggttatgatt ataatgctta atataaaaat
      541 aaaagatgca tttagagcaa ttattctgct tttacttcta ttactatcca tggttatatc
      601 tattgaaata agcagcatta tatcaatagg tgagtacata cccacacttg cactagcaaa
      661 tatatcagag acaaatagtg ttggctggaa aataaaacta ctaccttttc ttgtgttctt
      721 gtcatttatg attgcatcat tgattttgaa aagattgata aaagaaaaaa aaataagaaa
      781 tataatggtt gttgtttttt tattatcatt gttaatacca atatctccaa taaatgcttt
      841 tttttcttca tcatatgaag tttattcgga agcaaaagaa tacaatatgt caccacatga
      901 aataagtgat ataaagtcaa aatacacaaa agaaacagta acaaacgaca gttactcaca
      961 tgaaataata aatggaaacg gaaaaaatat tttagtaata tttattgaag gtatgtcttc
     1021 attggtaata tctaaggagc taaccccgaa tctatatgaa tttagaaaaa aatcaatatc
     1081 atttaacaac tattacaacc ataccgcagc tacatttaga ggtttaagag gacagttaac
     1141 ctcatcatat caaatgaccg gtgggtacca taaagataat tctggccttg gccaagcatc
     1201 taaagtagag atcgaacata aactaaatac ttacaattac ataacaaaat taccaatcat
     1261 acttgaggaa aatggttacc atacatattt tcaagcatct aattcaatag atgacccatt
     1321 gagtttaatg ctatcaactt taaagtttaa tcatatatac ggaagggagg actataacaa
     1381 aaagtcaggt gaactcacag acaaagagtc tttcgattta ttatttaaaa aattaaatga
     1441 ggctaaacaa cctttcttct atggtgttta tactgtaggt acacaccttg gcttagatag
     1501 ccctgatgta aagttttatg atgggagaaa tgaatattta aatcactttc atcaattaga
     1561 ccattggttt ggtgatttta tatctcattt taataagaca agcttatcga aagatacaat
     1621 tgtcataatc acaagtgacc atgcaagtta cccatcagaa gattacaaaa aaacattcaa
     1681 aactgataat actttttttg ttgatcagat tccattaata atatggagta atggtgttga
     1741 gttcaaagac attaatgcac acggtttaaa ttcactatct ttaacgccaa caatactaga
     1801 tattcttggg atcaaggagt cacacaacat gttcataggt gagtctctat ttgaaaaaga
     1861 aaaaaataaa aatgaactca actgtacatc tgcaataggt gacctgtatg tgaaaacgtg
     1921 taatggaaaa atggaaatcg agaaaagaaa agaaaaaaca gaattaataa aacaattgca
     1981 actttttggt gatagataat taaaaggcgg ttttacccca tgttgattat tagtccacgt
     2041 ggggttagtt taaaatgata ataaaactta ctttttttca ttcttgagcc gcctgtagaa
     2101 tgtggctcga ctaacaccta aagactccca ttcttttttt aaagttatat gaattaatat
     2161 taaaactacc tttaggccgt cctcctgaat tgcttagctt tcctccttta ccccctctct
     2221 ttctttgaat ttctgaacgg gcgtattttt tacatactca ataaaacgat cctctgaaaa
     2281 attattcgct gtccattttg agatgctttt tgatattcca agaacttcat tttcacttaa
     2341 tggtgatacg aattgtaaat tgtaggcaca ggctcgttca tagcaggctt gtaaccactg
     2401 ctcatactca ggccacccct gacggatggc acgatatgcc cacttgcggg ttttgtcgaa
     2461 cagagtgcag ttacggccta aaccataatc ggcggcaatt ttccagtgac tgtggttcgg
     2521 attcttgcaa attagccccg aatatcccgt gtcagctccc aattttctac gtagcgcatt
     2581 ctctacggca gctgcgtagc gtaacggttt catttttccg tcaggtgctg tgcgtataga
     2641 ggtttccagc gcataaagca ggtgcgcgtg tccgttttcg gggttggtga tggtcagcgt
     2701 aggtgcggga gcgttgcggt cgctccagtc aatggctgcg ccgatgcggt caacatcaaa
     2761 tcccagccag aacatggcgt gaggctga
//
LOCUS       DAJTNA010000309         2779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTNA010000309 DAJTNA010000000
VERSION     DAJTNA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.315"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..515
                     /locus_tag="OO161_004112"
     CDS             <1..515
                     /locus_tag="OO161_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5464945.1"
                     /translation="QLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTK
                     YFILNNGSVALAGRVLSIDATENGCVIHISLVNLLSTPISNIGFNATWGGEKPVDAKE
                     FARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPS
                     AENRQKRSKK"
     gene            559..>2779
                     /locus_tag="OO161_004113"
     CDS             559..>2779
                     /locus_tag="OO161_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5464946.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETVKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATDIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     "
BASE COUNT          842 a          638 c          667 g          632 t
ORIGIN      
        1 gtcaactgga aactattcaa aaacaacaaa ctggcataat aagcagtaca aagactttaa
       61 ctcacgtaat taaatccgtt aaagatcaac aaaacacttt tatttttacc gagtttaacc
      121 cagcaaaaac gaagtatttc atcctgaaca atggttcggt ggctttagcg ggtcgagtgt
      181 tatccattga cgccacagag aatggctgtg ttattcatat ttcactggtc aacttattaa
      241 gtacacctat ctcaaatatt ggttttaatg cgacatgggg tggtgaaaaa cctgtcgatg
      301 ccaaagaatt tgcccggtgg caacaattac ttttcaacac atcaatgaaa tccacattga
      361 aattattacc aggtcaatgg caagacatta atttgaccct gaagggtgta tcgcccaata
      421 acctgggata tctgaagtta gccatcaaca tggaaaatat tcagttcgac aatcttccct
      481 ctgctgaaaa tcggcagaaa agaagcaaaa aataaatgca gttagtacgc ttataacaag
      541 gaatagcgag catcattaat gaacaaaata tttaaagtta tctggaaccc tgcgacaggg
      601 aattatactg ttaccagcga aacggtaaaa agccgtggca agaaatctgg gcgcagtaag
      661 ctgttaattt ctgcgctggt tgcgggtgga atgttgtcgt cgtttggggc attggcgaat
      721 gccgggaatg acaacggtca gggtgttgat tacggtagtg gatcagctgg cgacggctgg
      781 gttgctatag gcaaaggggc gaaagcaaat acttttatga acaccagtgg ttccagtact
      841 gctgtgggtt atgacgctat agctgaaggc caatatagct ctgccatcgg gtcaaaaacc
      901 catgcgattg gtggtgcatc aatggccttt ggggttagtg caatatcaga aggcgataga
      961 agtatagcac tgggtgcctc ttcgtattca ttgggccaat actcaatggc cctcggccgt
     1021 tattcaaaag cattgggtaa attgtctatt gctatggggg actcttccaa agcggaagga
     1081 gcaaacgcca ttgccctggg aaatgccact aaagctactg atattatgag tattgctctt
     1141 ggcgacaccg ccaatgcgtc aaaagcgtat tcaatggcgc tgggagcaag tagcgtcgca
     1201 tctgaagaaa acgcaattgc cctggggcgt agcagtgtag ctagcggtac tgacagcctc
     1261 gcatttggca gacaatcact tgccagcgca gcgaacgcta ttgcgatagg tgctgagacc
     1321 gaagccgctg aaaatgcaac tgctattggc aataatgcga aggcaaaagg gactaatagc
     1381 atggcaatgg ggttcggaag ccttgccgat aaagtcaata ctatcgcatt aggaaatggc
     1441 agccaggctc tggcagataa tgcaatcgcc ataggccagg gcaacaaagc tgatggcgtg
     1501 gatgccatcg ctctgggtaa tggtagccag tcgagaggct taaacaccat tgccttaggc
     1561 acagccagta atgcaactgg tgataagagt cttgcgcttg gtagtaatag cagtgccaac
     1621 ggtattaact ctgtcgcgct gggcgcagat tccattgcgg atttagacaa taccgtctct
     1681 gtcggcaata gttcattaaa acgcaagatc gttaatgtga aaaatggcgc gatcaagtct
     1741 gacagttacg atgccattaa tggttcacag ctttatgcca ttagcgactc ggtagcaaaa
     1801 aggcttggag gaggggctgc agtagatgtt gatgacggta ctgttacagc accaacctac
     1861 aatttaaaaa atggtagcaa aaataacgta ggggctgcgc tcgctgtact tgatgaaaac
     1921 accctgcaat gggaccaaac caaaggcaaa tacagcgctg ctcatggtac tagtagccca
     1981 actgccagcg taatcaccga tgttgcggat ggcacgattt cagcctccag taaggatgcg
     2041 gttaacggtt cccaactgaa agctaccaat gacgatgtcg aagccaacac cgccaatatc
     2101 gctactaata ccagcaacat tgccacgaat acggcaaata ttgccaccaa taccaccaat
     2161 atcaccaacc tgacggattc cgttggtgac cttcaggctg atgccctgct ctggaacgaa
     2221 actaaaaagg cattcagtgc agctcacggc caggatacca ccagcaaaat caccaacgtt
     2281 aaagatgccg acctgacggc tgacagcact gatgctgtta acggctctca gctgaaaacc
     2341 accaacgatg ctgtggcgac gaataccacc aatatcgcca ataacacttc caatattgcc
     2401 actaacacca ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg
     2461 ctgaaatggg ataaggacaa tggtgtattc acggcagctc atggcaccga gaccaccagc
     2521 aaaatcacca acgttaaaga tggcgacctg acgactggca gcaccgatgc cgttaacggc
     2581 tctcagctga aaaccaccaa cgatgccgtg gcgacgaata ccaccaatat cgccactaac
     2641 accaccaaca tctctaatct gactgagacg gtgactaatc ttggtgagga tgcgctgaaa
     2701 tgggataagg acaatggtgt cttcactgca gctcatggca acaataccgc cagcaaaatc
     2761 accaatatcc tggacggca
//
LOCUS       DAJTNA010000310         2767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTNA010000310 DAJTNA010000000
VERSION     DAJTNA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.316"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            47..325
                     /locus_tag="OO161_004114"
     CDS             47..325
                     /locus_tag="OO161_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5464947.1"
                     /translation="MSDAVYRRLILMKAMSNITDCSVPDINRMLRFMFGKKRRAYVLN
                     NGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            312..842
                     /locus_tag="OO161_004115"
                     /pseudo
     CDS             312..842
                     /locus_tag="OO161_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1176..1697
                     /locus_tag="OO161_004116"
     CDS             1176..1697
                     /locus_tag="OO161_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5464948.1"
                     /translation="MDVYANSQRVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRSVVAELGDDSGWHFFSQRNTDNSITFAVNGQVIPLNYGNFDARYKYRT
                     EGVQDVRYGHEMYYSPGSNTVSWRFRAPSGHGLSGIAISDTGRDSADNVNGVYYRPLQ
                     KLINGTWYNVASV"
     gene            1700..2245
                     /locus_tag="OO161_004117"
     CDS             1700..2245
                     /locus_tag="OO161_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5464949.1"
                     /translation="MLHLKNITAGNPKTVEQYQLTKQYDVTWLFSEDGKNWYEELKNF
                     ASDTIKITYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     LDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKATYNAINWPERPDAAA"
     gene            2232..>2767
                     /locus_tag="OO161_004118"
     CDS             2232..>2767
                     /locus_tag="OO161_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5464950.1"
                     /translation="MPQLKGVIKTPTGEPLDGATITLTSIHNRAGILKSVFSHVTTQN
                     GEYDFPVLPGVYSVRLTQSTRRLSEIGVIRVYEDSTDGSLNDFLGATNIDLRPESLKK
                     FEELAQQAQQSAGSAAGNARQTAQDVTAAATARDDAQRFAEKARQDATVTAENRKATA
                     EDVTSTGANAAAAGQSAQ"
BASE COUNT          737 a          643 c          765 g          622 t
ORIGIN      
        1 tcaggcaccg ttttacaacg gaaaatcgga tacccggact gttgacctgt ctgatgctgt
       61 ataccggcgg ctgatactga tgaaagccat gtcgaacatt actgactgtt ccgttccgga
      121 tattaaccgg atgctgagat ttatgttcgg aaaaaaacgg cgggcttatg ttctgaataa
      181 tggtgggctg aggatgagtt acatctttga gtccgcgctc tcgttggcag aactggcgat
      241 tatccagtcg tcgggtgcac tgccgtcccc gccgggtgtt tatgtttcag tagttttaaa
      301 ggagtcccgt aatgaaggcc agtgataaac cccgccagct ggcggtcccc tttgcgagta
      361 ccggagataa aaaccgtatc ccggacaagg cgacacagca gaccagagag agcggtaatg
      421 ctgcgtatga ttcaggtttt cctccggtga ccatgacagc ggtctcagcg ggaggtatac
      481 cgccacacgg caaggatttt aacggtctga tgtacgatat taccgcagca atacggttcg
      541 cccaagctgg cggtttgtac acgtataatg ccggttttgc gggggccatt ggtggatatg
      601 caaaaggagc cattctcgcc ggagtcgcaa caacagcggt ctggctgaat accacagacg
      661 ataacctgac cgatcctgaa ggctccgaca gtgcgggctg ggtaaatctt cttgaggatc
      721 cgaaaaggat attcctgcgg cagaagaaca atctgtcaga ccttcagaat aaagggacgg
      781 cacgggataa tcttcaggtt tacagtaaag agcagtcaga tcaacgctat gttcatcggg
      841 aaggcgataa aataaccgga gagctgaaaa tccgtggtgt taatgcgctg aggattttca
      901 acgaagcttt tggcctgatt tttcgtcgtt cggaagagtg cctgcacctt attcccacca
      961 gtgaaggtca gggggaaaat ggcgatattg gtcccctgcg cccgttcacc attaatttgc
     1021 ggacgggtga aatatccatg tcgcataaag tgtctgttgg cggtggttcg caggtcaatg
     1081 gtgcgctggg tatcggcgtt cagaacgccc tgggggggaa ttcaattgtt cttggtgata
     1141 atgacaccgg atttaaacag aatggagacg gtattctgga tgtttatgct aatagtcagc
     1201 gtgtattccg ctttcagaat ggagtggcta ttgcttttaa gaatattcag gccggaactg
     1261 ccagaaaatt cacgttatcc agcgccaaca actccacgaa aaatgcagcg ttttatttgt
     1321 ggggtaatcc atccaggtct gttgttgcag agcttggcga tgattcaggc tggcattttt
     1381 tcagccagag gaatacagat aacagcatca cgttcgccgt aaacggacag gtaattccgt
     1441 taaattacgg aaacttcgat gcccgctata aatatcgaac agagggggta caggatgtac
     1501 ggtatggcca tgaaatgtat tacagccccg gcagtaacac cgtttcgtgg agatttcgcg
     1561 caccttcggg acacgggctg tcagggatag cgatatcgga taccggccgt gactcagcgg
     1621 ataacgtcaa cggcgtgtat taccgcccgc ttcagaaact gattaatggc acctggtata
     1681 acgtggcgag tgtttaacaa tgttgcattt aaaaaatatt actgcgggca atccgaaaac
     1741 cgtggaacaa tatcagctga caaaacaata tgatgtcacc tggctttttt cggaagacgg
     1801 aaaaaactgg tatgaagagc tgaagaattt cgccagcgac acaattaaaa taacttacac
     1861 cggagacggt cgcgtggtgt gggtcggtaa ggatgtgaca ggcattgaac cccgtaacgc
     1921 cagtgttatt gaagttcctg atattaccgc caaccgccgc attaccgcgc cgggttactg
     1981 gttttaccgc aatgatgaat ttgtccttga ctacagactc aaagcggaag atgagcgtga
     2041 tgcccttctg gctcaggtca gtgcccggac aggggaatgg gaagaagacc tgttgctggg
     2101 gttaatcagc gacgaagata aagaaaagct gaaagcctgt cgtatttacg cgaaatcgct
     2161 gcaggcgatg gatttcagca ccatcacaga taaagccaca tataacgcca tcaactggcc
     2221 ggagcgtccc gatgccgcag cttaaaggtg tgattaaaac gcccacggga gaaccgctgg
     2281 acggcgcaac cattacacta acctccatac acaaccgtgc agggattctg aaaagtgttt
     2341 tcagccatgt caccacacag aacggggaat atgacttccc cgttctgccg ggtgtgtaca
     2401 gcgttcgcct gacacaaagc acacggcgcc tttcagaaat cggtgtcatc cgcgtttatg
     2461 aagattcaac ggacggttcg ctgaatgatt ttctgggcgc aactaatatt gacctgcgtc
     2521 cggaatccct gaagaaattc gaagagctgg cgcaacaggc gcagcagagt gccgggagcg
     2581 cagccggaaa cgcacggcag acggcgcagg atgtgacggc agccgcaacg gcccgtgatg
     2641 atgcacaacg ttttgcggag aaagcccgac aggatgcaac cgtcacagct gagaacagaa
     2701 aggccactgc ggaagatgtg acaagcacag gagcaaatgc agccgcagcc ggacagagcg
     2761 cacagga
//
LOCUS       DAJTNA010000311         2757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTNA010000311 DAJTNA010000000
VERSION     DAJTNA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.317"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            143..418
                     /locus_tag="OO161_004119"
                     /pseudo
     CDS             143..418
                     /locus_tag="OO161_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            518..2458
                     /locus_tag="OO161_004120"
     CDS             518..2458
                     /locus_tag="OO161_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5464951.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     PSAQDKPASTDNTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARAEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDKVFPNPGNSNLGLMTA
                     FFEAYLDANYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKPQPSCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNAVDAKYQKLRTE
                     LHEARKNIPPKNPVDADKLLAASRGEFVEGISDPNDSKWIPGHHISSNEVKKTENEVR
                     QTEDKQHQNSEPEEGTPCNQTGEDNCPDCGAVMGDATYQETFDEKKPDEAQEEEPKKT
                     EKADDLLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGLCANPACIAYAPAIPGI
                     AMMIANKLENFGVTSDERLPDF"
     gene            2436..2732
                     /locus_tag="OO161_004121"
     CDS             2436..2732
                     /locus_tag="OO161_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR5464952.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
BASE COUNT          839 a          639 c          668 g          611 t
ORIGIN      
        1 ttatccggtc gataaagtta ttcaccatga taataacgaa atcccggcag gtctttaaaa
       61 cagttccgta ataaatatcc ggtttcattc ttatatgcca gcaatggcag gggttcgctc
      121 aaccttaatt aaggagaaaa acatgattac cagttatgaa gccactgttg taactactga
      181 tgacattgtt cacgaggtta atctggaagg aaagcgtatt ggctacgtga ttaagacaga
      241 aaataaagaa accccattca ctgtggttga tatcgacggt ccatcaggca acgttaaaac
      301 acttaacgat ggtgtaaaaa aatgtgtctg gtgcacatag gaaagaatct gcccgcagaa
      361 aaaaaaaacc gaatttctgg caactctgat tgcaatgaaa ttaaaaggtg aaatctgaaa
      421 aaaagaaagc ctgcacactg tgcaggcctg agtgaagaac ctgggacatt tattcatcac
      481 tcgcagtaat tttaatctga gttgaggtta aaaaacaatg aacaccgata aacaagttta
      541 cccactgtat tacgaagcaa aaaatgacaa agtaagaaaa cgtctcggta ttaaaggcgg
      601 tttttactgg gctgaagcga aaaaattatc cattgccatc tcccgtggtg ctgttgcgat
      661 tgacgatgct ggatacgatg aagatgactt caaaaaacct gttcgcgtca atttgcccgt
      721 tgttgatgac ctcccaccag aaggcgtgtt tgatacggaa ttctgcaacc gttacgaaaa
      781 aggcggggaa gatggcatca caatggtatt tatcgcgccc tcaccctctg cgcaggacaa
      841 accagccagc actgacaata ccaatgttaa tggcgaagac atgacggaga ttgaggagaa
      901 tatgctactc ccgatttctg gtcaggagct gcccattcgc tggcttgctc aacacggcag
      961 cgaaaaaccg gtaacgcacg tttcacgcga cgaactccag gcattacaca ttgcacgggc
     1021 tgaggaacta ccagctgtta ctgccctggc tgtttcgcat aaaaccagcc tgctcgaccc
     1081 gctggaaatt cgcgatctcc acaaactcgt gcgtgatact gacaaagttt tccctaatcc
     1141 tggcaattca aatctgggac tgatgactgc ttttttcgaa gcatatctgg atgccaacta
     1201 caccgatcgc ggtctgctga caaaagagtg gatgaaagga aatcgtgttt cacgcatcac
     1261 gcgcacggct tccggtgcga atgctggcgg cgggaacctc accgatcgcg gcgaaggttt
     1321 cgtccacgat ctgacgtcgc tggcgcgcga cgtagccact ggcgtactgg cccgttcaat
     1381 ggacgtggat atttataacc ttcatccggc acacgctaaa cgcattgagg aaattatcgc
     1441 tgaaaataaa ccaccctttt ctgttttccg cgacaaattc atcaccatgc ctggcgggat
     1501 ggattattcc cgcgccatcg tggttgcgtc agtgaaagaa gcaccgattg gtatcgaggt
     1561 catccccgcg cacgtcactg aatatctgaa caaggtactg actgaaaccg atcatgccaa
     1621 ccctgatccg gaaatcgtgg atattgcctg cggtcgctcc tctgccccga tgccgcaacg
     1681 tgtaacagaa gaaggaaaac aggatgatga agaaaaaccg caaccatctt gcgcaatggc
     1741 agatgaacag gcaacggctg aaacagtgga accggatgca actgaacatc atcaggacac
     1801 gcagccgctg gatgctcagt cacaggtaaa tgctgttgat gcgaaatatc agaaactgcg
     1861 gacagaactc catgaagccc ggaaaaacat tccgcccaaa aatcctgtcg atgcagacaa
     1921 attactggct gcctctcgcg gagaatttgt tgaagggatt agcgacccga atgattcgaa
     1981 atggattccc ggtcatcata tctcctcaaa tgaagtcaaa aaaacggaaa atgaagtgcg
     2041 acagactgaa gataaacagc accagaacag cgaaccggag gagggaacac cctgcaatca
     2101 gactggcgag gataactgcc ctgactgtgg cgcggtgatg ggagacgcaa cttatcagga
     2161 aacttttgac gaaaaaaaac cagatgaagc acaggaagaa gagccgaaga aaacggaaaa
     2221 agctgatgat ttgttgccgg agaacgctgg cagcgatcag cataatgata gcaataatga
     2281 aactggcgaa gatgaactca actggaaaaa acaggtgttg attgcggcgg tttacggttt
     2341 atgtgcgaat cctgcgtgca tagcctatgc gcctgcaatc cctggcatcg caatgatgat
     2401 tgccaacaag cttgaaaatt ttggagtgac cagtgatgag cgcttacccg atttttgatc
     2461 gtattgaaga aatggcatgg gctcgtcact accagcagat cgttcgtgaa gaaaaagaaa
     2521 cggaactggc ggacgacctg gaaaaaggtc tgcctcagca cctgtttgaa tcgctctgca
     2581 tcgaccattt gcaacgccac ggtgccaata aacaggcaat cagccacgca tttgatgatg
     2641 atgttgagtt tcaggagcgc atggcagaac acatccggta catggctgaa accattgccc
     2701 accaccaggt tgatattgat ttggaggact aaaatctatg gcgctttttc aacgagc
//
LOCUS       DAJTNA010000312         2751 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTNA010000312 DAJTNA010000000
VERSION     DAJTNA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.318"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            151..1317
                     /gene="nhaA"
                     /locus_tag="OO161_004122"
     CDS             151..1317
                     /gene="nhaA"
                     /locus_tag="OO161_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5464953.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEISKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGVSLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1383..2282
                     /gene="nhaR"
                     /locus_tag="OO161_004123"
     CDS             1383..2282
                     /gene="nhaR"
                     /locus_tag="OO161_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5464954.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2320..>2751)
                     /locus_tag="OO161_004124"
     CDS             complement(2320..>2751)
                     /locus_tag="OO161_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5464955.1"
                     /translation="NLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGEL
                     VFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSHDGTNEIQYGKSNYY
                     SFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          652 a          611 c          700 g          788 t
ORIGIN      
        1 ccgtcaaaaa ctcatctcac cgctgatggc gcaaattcta caaaggctcg taaaaaacga
       61 attattccta cactataatc tgattttaac gatgattcgt gcggggtaaa atagtaaaaa
      121 cgatctattc acctgaaaga gaaataaaaa gtgaaacatc tgcatcgatt ctttagcagt
      181 gatgcctcgg gaggcattat tcttatcatt gccgctatcc tggcgatgat gatggccaac
      241 agcggcgcaa ccagtggatg gtatcacgac tttctggaga cgccggttca gctccgggtt
      301 ggttcactcg aaatcagcaa aaacatgctg ttatggataa atgacgcgct gatggcggta
      361 tttttcctgt tagtcggtct ggaagttaaa cgtgaactga tgcaaggatc gctagccagc
      421 ttacgccagg ccgcatttcc agttatcgcc gctattggtg ggatgattgt gccggcatta
      481 ctctatctgg cctttaacta tgccgatccg attacccgcg aagggtgggc gatcccggcg
      541 gctactgaca ttgcctttgc acttggtgtg ctggcgctgt tgggaagtcg tgttccgtta
      601 gcactgaaga tctttttgat ggctctggct attatcgacg atcttggggc catcattatc
      661 atcgcattgt tctacactaa tgacttatcg atggcctctc ttggcgtcgc ggctgtagca
      721 attgcggtac tcgcggtatt gaatctgtgt ggtgtacgcc gcacgggcgt ctatattctg
      781 gttggcgtag tgttgtggac tgcggtgttg aaatcggggg ttcacgcaac cttggcgggg
      841 gtaattgtcg gcttctttat tcctttgaaa gagaagcatg ggcgttctcc agcgaagcga
      901 ctggagcatg tgttacaccc gtgggtggcg tatctgattt tgccgctgtt tgcatttgct
      961 aatgctggcg tttcactgca aggcgtcacg ctggatggct tgacctccat tctgccattg
     1021 gggatcatcg ctggcttgct gattggcaaa ccgctggggg ttagtctgtt ctgctggttg
     1081 gcgctgcgtt tgaaactggc gcatctgcct gagggaacga cttatcagca aattatggcg
     1141 gtggggatcc tgtgcggtat cggttttact atgtctatct ttattgccag cctggccttt
     1201 ggtagcgtag atccagaact gattaactgg gcgaaactcg gtatcctggt cggttctatc
     1261 tcttcggcgg taattggata cagctggtta cgcgttcgtt tgcgtccatc agtgtgacag
     1321 gacggtttac cggggagcca taaacggctc ccttttcatt gttatcaggg agagaaatga
     1381 gcatgtctca tatcaattac aaccacttgt attacttctg gcatgtctat aaagaaggtt
     1441 ccgtggttgg cgcagcggag gcgctttatt taactccaca aaccattacc ggacagatcc
     1501 gggctctgga agagcgcctg caaggcaaat tatttaagcg caagggacgt ggtctcgaac
     1561 ccagcgagct gggagaactg gtctatcgct atgccgataa aatgttcacc ttaagccagg
     1621 aaatgctgga tattgtgaac tatcgcaaag aatccaattt attgtttgac gttggcgtgg
     1681 ctgatgcact ttccaaacgc ctggtcagta gcgtgctgaa tgctgcagtg gtagaaggcg
     1741 agccgattca tcttcgctgc ttcgaatcca cccacgaaat gctactggag caattaagcc
     1801 agcataaact ggatatgatc atttccgact gtccgataga ctctacgcag caggaaggcc
     1861 tgttctccgt gagaattggc gaatgtggcg tgagtttctg gtgtacaaat ccaccaccag
     1921 aaaaaccgtt cccggcttgt ctggaagaac ggcgactttt gattcctggg cgacgttcaa
     1981 tgttagggcg caaattgctt aactggttta actcccaggg attaaacgta gaaatcctcg
     2041 gcgagtttga tgatgccgcg ttgatgaaag cttttggtgc aatgcacaat gcaatcttcg
     2101 ttgccccaac gctttatgca tatgactttt atgccgataa aactgtcgta gaaattggtc
     2161 gcgtcgagaa tgtgatggaa gagtaccatg ctatttttgc tgagcggatg attcagcacc
     2221 cggcggtaca gcgaatctgc aatacggatt attctgcgct ttttagtcca gcggcgcgtt
     2281 aatcggcagc tcccccaaag ttaaagtgga ggagatagat tagttgtaca taaccacgat
     2341 tttgactcgg ctcattattt gcccgcttga gacattattt ccatatgtac gcgggcgaat
     2401 aaatagagga atctgattac ttccttcatg gggatgctga aaagagtagt aattgctttt
     2461 accatactgg atttcattcg taccatcatg ggagagttga aagcctactt gacccgcacc
     2521 gtcctcactg ttttctaact cattttttaa tatgctggtt cctgactcct gtatcgtcac
     2581 tggttcaaag taatattcaa catcactggg ttcaaaaact aattcacctt gctcgccctg
     2641 gatgcagtca aatcttatat gatgaaactg ataattttca ttattgatac gagacagtac
     2701 aaatcgtcca gtgtcgatgg attgatcttc aaaaccacag gtcattaaat t
//
LOCUS       DAJTNA010000313         2679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTNA010000313 DAJTNA010000000
VERSION     DAJTNA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.319"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            45..770
                     /locus_tag="OO161_004125"
     CDS             45..770
                     /locus_tag="OO161_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5464956.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLF
                     QKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQSIY
                     RKEYATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKV
                     INESGVKYDVIHFGACRVNREEK"
     gene            complement(996..1319)
                     /locus_tag="OO161_004126"
     CDS             complement(996..1319)
                     /locus_tag="OO161_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR5464957.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1603..1797
                     /locus_tag="OO161_004127"
     CDS             1603..1797
                     /locus_tag="OO161_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5464958.1"
                     /translation="MNKQQQTVLNMAGFIKGQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTDR"
BASE COUNT          863 a          659 c          571 g          586 t
ORIGIN      
        1 aaaaaaaaac aataaaaaac accgcaaaaa tggattgtta acaaatgaaa atactcaatt
       61 cgaatctcat aacaaacata tcatcaacaa taagaaatca ccttctaaca tcatccaaaa
      121 atataaaaac acacccatca ccaaacatgg acgtaacaag tcatcgtcgt ttattccaga
      181 aagacggaat aggattgagc caaacaggaa aagcaacaca acttgtcatt atgtcgcatg
      241 gtggatggaa agagatctcc catccacaaa cactattcac gcgtcaaaaa ggcgatggat
      301 ggactgtagt ccccaaaaac ctaagaattg atttttatac aaaagacaac gacttcacaa
      361 agggactaag cgttctttca gaggtaaaca aacgccatac tgaagctcaa aaagggttaa
      421 caccatcact aaacataagc aaagatgacc taaaagttct agcgaacgcg cgcaatatac
      481 ctcaatcaaa attagaagaa gaaatgatgt cacaatccat atatagaaaa gaatacgcaa
      541 caagtaatga gaagataaaa aactatgctc tatattacca tgaacaagcc caaaatatta
      601 tacaaagaca tcaagatggc ttaggaaaca aaaatattga tatagcattt ataactgata
      661 aaaatcataa gaagcacctt tctgacatat ttaaagtaat aaatgaatca ggagtaaaat
      721 atgatgtaat tcactttggc gcttgccgag taaatagaga ggagaaataa tcatcattta
      781 ccataatccc ttaattgtac gcaccactga aacgcgctga acgcgattct gacgcagaca
      841 ccacaaaaat agatgtttta ccccggaaaa acaggaagga tgcgtcagga tcgttttcag
      901 ctcgctgcat agctatgcat gaaagcgaat ggagatcact ttgggagctt accgtgttca
      961 taccgtctgt tttcaacagt tttctccccg ggaagctaat ctgccataag cctggataac
     1021 agggcacggt gatactccgt aatagcgatc agcacctccg catactccct gtccccgata
     1081 cgcttgccgt cgataaagag ttttttctgt aacgccccca gtctcataac ttctttcagt
     1141 acccgatcat cagtcagtga gttaacgttc ttaccgagag ctgccgcccg taaaaaaccg
     1201 gaaacggtct tgccgcattc agcagccctt tttctgattt cctggtcttc ttctgccgtc
     1261 agacgaacag ggcgactgat agcccgcctg cgcgtattac ttccacttcg ttttgtcata
     1321 aaacatggct ccgtatctga catgggtgtc ggggcaaagc cctgaccagg gcaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt gcacatcctg tcctgtcagc aagctcgaat
     1441 taccgacaaa ggcacggcag ttttttggca gttacagtgt tgtctgacag aaatgcccgt
     1501 caaaaagcca ggcggcagaa tagcggcagc aaaacagtcg aaaagtggta cgcgatataa
     1561 tcacataacg cagatgacag acagaacgga gaaaacgaaa agatgaacaa gcagcagcaa
     1621 accgtactca acatggcggg attcataaaa ggccagagcc tgacgctgct cgaaaaactg
     1681 gacgcactcg atgctgacga gcaggccacc atgtgtgaga agctgcacga actcgcagaa
     1741 gaactccaga acagcataca gacacgcttt gaagtagaaa atagcacaga tagataacgc
     1801 tcaggacagg atgccgaacg gcacaacaga gcgtaaggag aaaatgtcac ataaggcgct
     1861 cttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggtgt
     1921 cagctcactc aaaggcggta atgcggttat ccacagaatc aggggataac gccggaaaga
     1981 gcatgtgagc aaaaagccga aggcagggac aaaggccgcg caggtggcgt ttttccatag
     2041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2221 ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2341 tgagtccaac ccggtaagac acgaattatc gccactggca gcagccactg ataaccgaat
     2401 tagaggagta agacttgaag tcatgcgcct gttaaggcta aactgaaaga acaagttttg
     2461 gtgactgcgc tcctccaagc cagttacctc ggctcaaaga gttggtagct ctgagaacct
     2521 tcgaaaaacc gccttgcagg gcggtttttt cgtttacagg cagtagatta cgcgcagaaa
     2581 aaaaggatct caagaagaac ctttaatttt ttttcacact tggtatccta ctttaaccca
     2641 taaaataaac acttacaccg acacgccctt aaacaaaag
//
LOCUS       DAJTNA010000314         2608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTNA010000314 DAJTNA010000000
VERSION     DAJTNA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.320"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..73)
                     /gene="csgC"
                     /locus_tag="OO161_004128"
     CDS             complement(<1..73)
                     /gene="csgC"
                     /locus_tag="OO161_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5464959.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDV"
     gene            complement(132..587)
                     /gene="csgA"
                     /locus_tag="OO161_004129"
     CDS             complement(132..587)
                     /gene="csgA"
                     /locus_tag="OO161_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR5464960.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNTDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(628..1083)
                     /gene="csgB"
                     /locus_tag="OO161_004130"
     CDS             complement(628..1083)
                     /gene="csgB"
                     /locus_tag="OO161_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5464961.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSAFN
                     QAAIIGQAGTNNSAQLLQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1837..2487
                     /gene="csgD"
                     /locus_tag="OO161_004131"
     CDS             1837..2487
                     /gene="csgD"
                     /locus_tag="OO161_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5464962.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     HSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNDIKILLLNTPEDYPYRDIENWP
                     HINGVFYSMKDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2492..>2608
                     /locus_tag="OO161_004132"
     CDS             2492..>2608
                     /locus_tag="OO161_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="chaperone-like protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgE"
                     /protein_id="HCR5464963.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSI"
BASE COUNT          744 a          572 c          505 g          787 t
ORIGIN      
        1 acacatcccc ttgctgggtc gtattaaagg ttatctgact ggaaagtgcc gcaaggagta
       61 ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt ttctgtaata
      121 caaatgatgt attagtactg atgagcggtc gcgttgttac caaagccaac ctgagtcacg
      181 ttgacggagg agttagatgc agtctggtca actgcagcac cgttgccacc accgaactgt
      241 ttaaccgtca tttcagaatt tttgccgttc cactgatcaa gagtagcgct attaccgaag
      301 ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc tgcaccatta
      361 ccgccgccat gctgggtaat agtcaagtca gtgttacggg catcagtttg cagagcaagt
      421 gcagagttac cgccaccgta ctggtaaatg ttcagctcag aatttgggcc gctattatta
      481 ccgccaccac cgtggttacc gccgccgccg tactgaggaa caacacctgc cagagcgcta
      541 ccggagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
      601 cggattgatt taaaagtcga atggaaatta acgttgtgtc acgcgaatag ccatttgcga
      661 ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
      721 accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
      781 atcgttggca ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctggtcaat
      841 ctttgcccga ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agcctccctg
      901 cagtaactga gcactattat tcgtcccagc ttgaccaatt atggctgcct gattaaatgc
      961 agacttactc aattcattta ccgcgaagtt atattctgaa ttagctaaat cataacctgc
     1021 tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca atttgttttt
     1081 catgttgtca ccctggacct ggtcgtacat ttaagaaatt aaatcatttc aacttggttg
     1141 ttaacgcaac ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc tgcggaaata
     1201 tttttaataa ctcacccgcg cgttgtattt ttcttttttt gttcatacaa aagtattaaa
     1261 acccacgtaa atacgctgtt atctacgcaa aaaatatttt gttttctttt aaatctccgt
     1321 tttccgctat caaaaatcac cagacagtca tttttcttgc ccgtcgctga ttgctgcgat
     1381 atgtcttgcg cacaagccgt gacaaacctc gtctttttgg agtaaaaatt tgtccacgga
     1441 ggtgcggaga aaaaacaaga acgttttaca tgacgaaagg actacacaga aatatttttt
     1501 atatgtatta ttagtaagtt atcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1561 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaaggt taacatttta
     1621 atataacaag ttacatttag ttacatgttt aacacttgat ttaagttttg taatggctag
     1681 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1741 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1801 caggtgtgcg atcaataaaa aaagcggggt ttcatcatgt ttaatgaagt ccatagtatt
     1861 catggtcata cattattgtt gatcactaaa ccttctttgc aggcgacagc tctcttgcag
     1921 caccttaaac aatcgctggc aattacagga aaattacata atattcaaca ttctctggac
     1981 gatatctctt caggctctat tattcttctg gatatgatgg aagcagataa aaagcttatc
     2041 cattattggc aggatacttt gagcaggaaa aacaacgata tcaaaatatt gttgctaaat
     2101 acgcctgaag attacccgta ccgcgacatt gaaaactggc ctcatatcaa cggcgttttt
     2161 tattccatga aggatcaaga acgtgttgtc aatgggttgc aaggcgtcct gcgcggcgaa
     2221 tgctacttta cgcaaaagct tgccagctac ttgattacgc attcaggtaa ctatcgttat
     2281 aacagcacgg aatcagccct ccttactcat cgggaaaaag agatcctgaa taagctgcgt
     2341 atcggcgcgt ctaataacga gatcgctcgt tcgttgttca tcagcgaaaa tacggttaaa
     2401 acgcatcttt ataatctttt caagaagata gccgtaaaaa accggacaca agcggtttcc
     2461 tgggcaaacg ataacctcag gcgataaagc catgaaacgt tatttacgct ggattgtggc
     2521 ggcagaattt ctgttcgccg cagggaatct tcacgccgtt gaggtagaag tcccgggatt
     2581 gctaactgac catactgttt catctatt
//
LOCUS       DAJTNA010000315         2603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTNA010000315 DAJTNA010000000
VERSION     DAJTNA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.321"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..345)
                     /locus_tag="OO161_004133"
     CDS             complement(<1..345)
                     /locus_tag="OO161_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 family protein"
                     /protein_id="HCR5464964.1"
                     /translation="MPIPLSKDVQINPGVLAVAGNAVDLNGLLLTSNPLLPVGTVVPF
                     SSPDDVAAYFGALSDEYARAQLYFQGFKNATRTPGQLLFSRFSPAPSAAWLRSGSFKG
                     ATVEQLQKLSGTL"
     gene            complement(326..871)
                     /locus_tag="OO161_004134"
     CDS             complement(326..871)
                     /locus_tag="OO161_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464965.1"
                     /translation="MPSGVTLSVTESALYQALGDYHRGLFSDAGIERTQQNQVPMPQG
                     NFITMTGIDVTGLSTAVVAYSAPAQAGEGSQHITRTTKWRCQLDFYGPHAADNAQVLA
                     TLFRSEFSVRLFRQGGGLISPLYCSEPFNTTFPNGQQQYEPRWTLDIQMQINPVVTTS
                     LMFFDNVITRTTEADNANSTQ"
     gene            complement(856..1221)
                     /locus_tag="OO161_004135"
     CDS             complement(856..1221)
                     /locus_tag="OO161_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464966.1"
                     /translation="MNFHGIVSGAVRRVNPFTDALVYRSRGSTQQADYSRVPEYEDPV
                     SVRGQKQAVTQADLRHLDNLNQQGVFATLYTDGNWCGLNRARQQGGDKFVIGDETWLV
                     IEVLEIWPDWTRVIVCLQV"
     gene            complement(1218..1802)
                     /locus_tag="OO161_004136"
     CDS             complement(1218..1802)
                     /locus_tag="OO161_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464967.1"
                     /translation="MVNKVTGGKQFRQKLKQVAANLSLGKKLKVGFLEGATYPDGTSV
                     AYIAAVQEFGGRAVIPAREQTLHFRYNEKTGETGHRFVKAGKGNFVQDVVIPEHTVTI
                     PPRPFFRKMIEHKSPEWGEKMATLLRANDFDTATALVCMGEHIKGQLQMSVRDWESPS
                     NAASTARQKGFNNPLIETGHMMDSVDYSVDGGKK"
     gene            complement(1796..2251)
                     /locus_tag="OO161_004137"
     CDS             complement(1796..2251)
                     /locus_tag="OO161_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HCR5464968.1"
                     /translation="MGTVTFDCRMFVSLYPEFSTVTPELLTAVFNRATALYLDNTDDS
                     PVTDLKEREQLLFLLVAHLCALRGLGNGRDGQAGLVGRITSASEGSVSVSVDNSGSND
                     ASWWYLQTPWGADYWQATAPYRSMQYHSGGAPSRYPGHYYRGYGRGRRW"
     gene            complement(2257..2586)
                     /locus_tag="OO161_004138"
     CDS             complement(2257..2586)
                     /locus_tag="OO161_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464969.1"
                     /translation="MGCKLPNGLVLEVGQKRVQVAGWRNNAVKIVGGYGLTQVDKVFF
                     DAWLAEHGQQPYVKNGVIFAQDKANSAVSQATEQKAVKSGLEPLPQKNPAPGVNRNDE
                     VMGKPQE"
BASE COUNT          627 a          766 c          626 g          584 t
ORIGIN      
        1 cagcgtgccg gaaagttttt gtagctgttc aacagtcgcg cccttaaacg aaccgctacg
       61 taaccaggcc gccgatgggg caggactgaa acgggaaaac aacaattgcc ccggcgtcct
      121 ggtggcattt ttgaagccct ggaaataaag ctgagcgcgt gcgtactcat cggataatgc
      181 accaaaatac gcggccacat catccggaga ggaaaacgga accacagtgc cgaccggaag
      241 taacggatta ctggtcaaca gcaggccgtt aagatcgacg gcattacccg ccacagccag
      301 cacaccggga tttatctgta catctttact gagtggaatt ggcattatca gcctccgttg
      361 tccgggtgat cacgttgtca aaaaacatca gggaagttgt gaccacaggg ttaatctgca
      421 tctgaatatc aagcgtccag cgcggttcat actgttgctg accgttgggg aatgtggtgt
      481 taaagggctc tgagcaatac agcggggaaa tcagcccacc gccctgccgg aaaagccgta
      541 cggaaaattc agaccggaaa agcgttgcca gcacctgagc gttatctgcc gcgtgagggc
      601 cgtagaaatc aagctggcaa cgccattttg tggtacgggt gatatgctga gagccctcac
      661 cagcctgcgc tggcgcagaa tatgccacta ccgcagtgga taatccggta acatcaatgc
      721 ccgtcatggt gatgaaattc ccctgaggca tcgggacctg gttctgctgc gttcgttcaa
      781 tcccggcatc agaaaaaagc ccccggtgat aatcaccgag ggcctgataa agagcgcttt
      841 ccgtaacgga gagggtcaca cctgaaggca tacaataacc ctcgtccagt caggccagat
      901 ttccagtacc tcaatcacca gccacgtttc atcgccaata acaaatttat cgcctccctg
      961 ttgccgggca cggttaagcc cgcaccagtt accatcggta tacaatgtgg cgaaaactcc
     1021 ctgctggtta agattatcaa gatgacgtaa atcagcctgg gtgacagcct gcttttgccc
     1081 cctgacggaa accggatctt catactcagg tacgcgggaa taatctgcct gctgtgtact
     1141 cccgcgggaa cgataaacca gcgcgtctgt aaagggattt acccgacgta ctgcgccgga
     1201 aacaataccg tgaaagttca ttttttgccc ccgtctacag aataatcaac gctgtccatc
     1261 atatgtccgg tttcaataag cgggttgtta aaaccctttt gccgggcagt ggatgcagcg
     1321 ttggacggac tttcccagtc gcgaacagac atctgcaact gccctttgat atgttctccc
     1381 atacacacca gagcggtcgc agtatcaaaa tcattcgccc gtaataaggt cgccattttt
     1441 tcgccccatt cgggactttt atgttcgatc atcttacgga agaacggacg ggggggaatg
     1501 gtgaccgtgt gctcaggaat aaccacatcc tgaacaaaat tccctttacc ggctttgaca
     1561 aagcggtgcc cggtttctcc cgttttttcg ttatagcgaa agtggagcgt ctgctcacgg
     1621 gcgggtatga ccgctcttcc accaaactcc tgaactgcgg caatataagc cactgacgta
     1681 ccgtcagggt aagttgctcc ctcaagaaaa cccactttga gttttttacc caatgaaagg
     1741 ttcgcggcaa cctgtttcag cttctgccgg aactgtttgc cacctgtgac tttatttacc
     1801 atcgacgccc cctcccgtat ccccggtaat aatgccccgg atatcgcgaa ggtgcccccc
     1861 cggaatgata ctgcattgag cggtatggcg ctgtcgcctg ccagtagtca gcaccccagg
     1921 gggtctggag ataccaccac gacgcatcat tactgccgct attatccacc gataccgata
     1981 cagaaccttc cgacgcactg gtgatacgcc ccaccagacc agcctgcccg tctctcccgt
     2041 ttcccagccc ccgcaatgcg cacagatgcg caaccagcag gaacagaagc tgttcccgct
     2101 ccttcaggtc ggtaaccgga ctgtcgtccg tgttatccag gtacaacgcg gtcgcccggt
     2161 taaacaccgc cgtcagtagc tccggtgtca ccgtggaaaa ctccgggtac agactgacaa
     2221 acatccgaca gtcaaacgtc accgtaccca tagcgtttac tcctgaggtt tacccatcac
     2281 ttcatcattg cggttaacgc ccggagccgg atttttctgc ggcagcggtt caaggccgga
     2341 tttaacggct ttctgctccg tagcctgcga gactgcactg ttcgccttat cctgcgcaaa
     2401 aataacgcca tttttcacat aaggttgctg tccgtgctcc gccagccaag cgtcaaaaaa
     2461 caccttatcg acctgcgtca gaccatagcc ccccacaatt ttaaccgcgt tattccgcca
     2521 gcctgccacc tgaacccgtt tctgacccac ttccagcacc agaccgttcg gcagtttgca
     2581 gcccactgtt accatttcag cct
//
LOCUS       DAJTNA010000316         2573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTNA010000316 DAJTNA010000000
VERSION     DAJTNA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.322"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(153..1007)
                     /gene="rclR"
                     /locus_tag="OO161_004139"
     CDS             complement(153..1007)
                     /gene="rclR"
                     /locus_tag="OO161_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCR5464970.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1233..2558
                     /locus_tag="OO161_004140"
     CDS             1233..2558
                     /locus_tag="OO161_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCR5464971.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          737 a          580 c          600 g          656 t
ORIGIN      
        1 aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag taagttggca
       61 gcatcaccaa aaaagttgga aatataaata gctgaaaggc tcacaacacc cggaatgcgg
      121 attatctttg ctccgattcc tggcggtttt attcagggtg caagctgtct gaccctttcc
      181 cgatattctc ccggagtaca accaaactcg cggacaaacg ccttgtgaaa agatgattcg
      241 ctggcatagc ctactgactc agcgatcacc acaacaggga gcgtttcccg ggaaaacatc
      301 tgggccgcta tttgtagacg caactttgtt aatacagcca gcggcgtggt tccggaaaca
      361 tcacggaaaa gctgggcaaa acttgcccgg gacatgtggg cgatgctggc cagcgattcg
      421 acggtccaag cgtgtcctgg catttccagc atttgctgta ttaccgcacc aagacgtgga
      481 tgcagaagca aactgagaat gtttttctct gtattaacct gtgcaatcca ctcacgcacc
      541 gcgagggtaa agaatgtagc gcagatctgg ctacacagtg catccactcc cggcatcgcc
      601 gatctggatt cctgttgtaa aaacgggatc gcctccctca gccagttata ttccacgctg
      661 tggttaaccg gtgctaaaaa cagcgtttcc ggcaaagacg ttaaaaaata acgcgccgaa
      721 tgttgcaacc gaagagtacc acagacaata caggtcgatt cattatcgac atgactcagg
      781 cgatgagctg aattttgtgg tagcaggacc acatttcccg ggcgtaatgt aaaaatctct
      841 cccgtcggca tttccagctt cgccgctcct tgcgttaacg catgccaacg aataaccgat
      901 aattccccgg caccatgtgg aagctgccag tcacttccta acacgcaatt cttatcgatc
      961 gttccttgtg gagcgttaag catcaacaaa cggctaaggg catccatatc agactcctga
     1021 gtggtttttg attacaaaca ccaacaatct tagtaaataa ctaatctaat accatcaaat
     1081 agttaatcat gatttttgtt ttgtgattct gattaagaaa aagaaaatac cagacgataa
     1141 gacaaaatat caagcgttgt gaagaaatgg tatttgctct tttgcgtcta tagtcatgat
     1201 gtcaaatgaa cgcgtttcga caggaaatca tcatgaataa atatcaggca gttattattg
     1261 gttttggcaa ggctggaaaa acattagccg tcacgctggc aaaagcaggt tggcgagtgg
     1321 ctctcatcga acaatcaaat gcaatgtatg gcgggacctg tattaatatc ggctgcatcc
     1381 caactaaaac attggttcat gacgcacagc agcacacaga ttttgtccgt gccatacagc
     1441 gtaaaaatga agtggttaat tttttacgta ataagaattt tcataatctt gcggatatgc
     1501 ctaatatcga cgtgatcgac ggccaggcgg agtttatcaa taatcatagc ctgcgtgttc
     1561 atcggcctga gggaaatctg gagattcatg gcgagaaaat ttttattaat accggtgcac
     1621 aaaccgtggt tccgccaatt cctggaatta ccaccacgcc aggagtatat gacagcaccg
     1681 gattacttaa tctaaaagaa ttgcctgggc atttaggtat tttgggcggc ggatatattg
     1741 gcgttgagtt cgcctctatg ttcgctaatt ttggcagcaa ggtaaccatt ttagaagcag
     1801 cttcgctgtt tttgcctcgg gaagatcggg atattgctga taatatcgcg acgattttac
     1861 gcgatcaggg cgtcgatatt atcctcaatg cccatgtgga gcgaatcagt caccatgaaa
     1921 atcaagtgca agtgcatagc gagcacgccc aactggcggt ggatgcactg ttaatagctt
     1981 ccggtcgtca accggctacc gcttcgttac atccagaaaa tgccggtatc gcagtaaacg
     2041 agcgcggggc aattgtcgtt gacaagcgat tacataccac cgcagacaat atttgggcga
     2101 tgggagatgt taccggcggg ctgcaattta cttacatatc actggatgat taccgcattg
     2161 tacgtgatga gttactgggt gaaggcaaac gtagtactga tgatcggaaa aatgtgcctt
     2221 attccgtatt tatgacaccg cccctgtcca gggttggtat gacagaagaa caagccagag
     2281 agagtggtgc tgatattcag gtggtgacat tgcctgtagc tgcaattccg cgtgccagag
     2341 tgatgaacga tacccgtggg gtattaaaag cgattgttga taataaaacc caacgtatat
     2401 taggggcatc actgctgtgt gttgactccc acgagatgat taatatagtg aaaatggtga
     2461 tggatgccgg gctgccttat agcatattac gcgatcagat atttactcat ccgtcgatga
     2521 gcgaatcact caatgatcta ttttcattag tcaaataaac tcaaaatcag acg
//
LOCUS       DAJTNA010000317         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTNA010000317 DAJTNA010000000
VERSION     DAJTNA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.323"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..370
                     /locus_tag="OO161_004141"
     CDS             <1..370
                     /locus_tag="OO161_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5464972.1"
                     /translation="KELTGKALFMALRSQRPPAGLIHHTDRGSQYCAYDYRVIQEQSG
                     LKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFKSRDEAISVIREYIEIFYNRQRRH
                     SRLGNISPAAFRIKYYQMTA"
     gene            414..1214
                     /locus_tag="OO161_004142"
                     /pseudo
     CDS             414..1214
                     /locus_tag="OO161_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1329..1670
                     /locus_tag="OO161_004143"
                     /pseudo
     CDS             1329..1670
                     /locus_tag="OO161_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1653..2490
                     /locus_tag="OO161_004144"
                     /pseudo
     CDS             1653..2490
                     /locus_tag="OO161_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          648 a          622 c          755 g          547 t
ORIGIN      
        1 gaaagagttg acgggtaaag ccctgtttat ggcgctcagg agccagcgcc cacctgccgg
       61 gctaatccac cacaccgatc gaggttcaca gtactgcgca tacgattacc gggtcataca
      121 ggagcagtct ggtctgaaaa catcaatgtc gcgtaaaggt aactgttacg acaacgctcc
      181 gatggaaagc ttctggggaa cgctgaaaaa tgaaagcctg agccactatc gttttaagag
      241 ccgggatgag gccatctcag taatacggga atacattgag attttctaca atcgtcagcg
      301 tcgtcactct cgtctgggga atatctcccc ggcagccttc aggataaaat attatcagat
      361 gactgcttag aaaaagaaca aatggtggtg tccgctattg ccagtacacc tcagttctgg
      421 gccttgcccg tagcagttat ttttatcacc gggctgcact gaaagccggt gataaatacg
      481 ccacgatacg taagatgctg acagatatct ttaacagtaa ttaccagtgt tatggctatc
      541 gtcgcctgca tgcgatgctc aggcatgagg gggggcggct atcagaaaag gttgtacgca
      601 gacttatggt ggaggaacag cttgtcgtca gtcgtaaccg tcgtcgttgc tacagctcat
      661 attgcggaga aatcggaccg gctccggata accttatcgc cagagatttt aaggcggagc
      721 aacctaatca gaaatagctg acagatatca cggagttcca gctccctgca ggtaaagtct
      781 ggctgtcacc agtggtggac tgcttcgatg gaaaagttgt gagctggtct ctcagtacac
      841 gccctgatgc tgaactggtc aacactatgc tggatagcgc tgtcgaaacg ttaaatgctg
      901 gcgaacgacc ggtgatacac agtgacagag gtgggcatta tcgctggcca ggctggctgg
      961 aaagagtgaa tgcagcaggt cttattcgct ccatgtcccg taaaggatgt tcacctgata
     1021 atgctgcatg cgaaggcttt ttcggcagac tgaaaacgga aatgtattat gggcgtaaat
     1081 ggtcgggcat cacgccagaa aagttcatgc agcaagtgga tgcttacatc agatggtata
     1141 acgagcggcg tataaaatta tcgctgggtg ccgtcagccc caaaatgtac cgccaacaat
     1201 gcgggctgga atgataaagc agtccaggaa atcgtccgca tccccccttc accatttgga
     1261 ttgtccgctc cacccaacat gttgtttcct taaggttcct acaccacaaa ggaaactctc
     1321 atgctgcgca gtggatggct gacgttgccg acaggcggga acttcgccag ttcaggcaga
     1381 caccggaaca gcgcttcacg caggaacagg agcatctgca gccgctgctg ggtacggact
     1441 tcgatatccg tcatgtctcc tgggatggct atatcgaggt tggcgggaac cgttacagtg
     1501 tgccggaaag cctgtgtggt cagctggtat caatacggat ctcgctggat gatgaactgc
     1561 ggatctacag taatgagcaa caggtagcat cgcaccgtct ctgttcggct gcatacggct
     1621 ggcagaccgt gccggagcat cacgctccac tctggcagca ggcttcggag cgcatggccg
     1681 aacggctggg ggagatacag aaacgggtaa tcactgtctg tgaccgggaa gcggatatct
     1741 ggcattacct gtattataaa gtcagccacg gacagcgtgg tgcgtgctgc acagaatcgc
     1801 cggctggaag aggcacccgg caagctcttc gaactgccgg aagtcctggc aaccgcagga
     1861 agccacacgc tgaatgtgat gcaaaaaggc gggcgggcag cccgtcaggc ccggatgttc
     1921 atcagttaca gcgaagtcag cataaaaaat cccgacaaca gcggccaggc gctcccgctc
     1981 acgtatgtct gttgccggga gcaggcagag gacggtgcct gctggcatct gctgacgtca
     2041 ggaaaagcgg cgagtgccgc cgatgcacga cgtattgtca gccattacga gcgacgctgg
     2101 ctgacagagg aataccacaa ggcgtggaaa agtggtggta catggaatcg ctgcgaatgc
     2161 agacccggga taaccttgag cgcatggtgg ttatccaggc gtttatagcg gtacgggtgc
     2221 tgggcctgcg gcagggaggt gtcagtgaag aaacacaaaa tgacagctgt gagaaaatac
     2281 tgacgccgac agagtggaaa ctacagtggg ttaagctgga aggaaagccg cttccggtgc
     2341 aggcaccgac cctgaaatgg gcctggggcg atggtatgac agcaaacgca caggtcgtcc
     2401 cggttggggc gtcatgtggg atggctggtc agacttcagg atatggttga gggttacctg
     2461 gtgatgaagt ctcttgatca ggagctctga tcaagagaca gcagctttgg ggggtgcccc
     2521 tattaagttg tcaagcatgt tatgacccct gcggggtata aaaagtcccg tc
//
LOCUS       DAJTNA010000318         2566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTNA010000318 DAJTNA010000000
VERSION     DAJTNA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.324"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..938
                     /locus_tag="OO161_004145"
     CDS             <1..938
                     /locus_tag="OO161_004145"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5464973.1"
                     /translation="DVVDLIAQVDLVTTAVGPVVLERIAPAIAKGLVKRKEQGNESPL
                     NIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRIVPPSASATNDPLE
                     VTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNTGHAITAYLGKLAG
                     HQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQRILGRFENPYLKDD
                     VERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHFRSEDDPQAQELAA
                     LIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            938..1525
                     /gene="mtlR"
                     /locus_tag="OO161_004146"
     CDS             938..1525
                     /gene="mtlR"
                     /locus_tag="OO161_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5464974.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1537..1746)
                     /locus_tag="OO161_004147"
     CDS             complement(1537..1746)
                     /locus_tag="OO161_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464975.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            2006..2392
                     /locus_tag="OO161_004148"
     CDS             2006..2392
                     /locus_tag="OO161_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5464976.1"
                     /translation="MYLYQKPSHERSRKNEIKRLSDRLDAIRHQQADLSLVEAADKYA
                     ELEKEKATLEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGL
                     VVVHPMTALGREMGLQEMTGFSKTAF"
BASE COUNT          685 a          634 c          650 g          597 t
ORIGIN      
        1 atgatgtcgt tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg
       61 tgctggaacg tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta
      121 atgaatcccc gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga
      181 aaggccatgt gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg
      241 gctttgtcga ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc
      301 cgctggaagt gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag
      361 gcgcactgcc gaacatccca agcatggagt taaccgacaa cctgatggca tttgtcgaac
      421 gtaaactctt caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg
      481 gtcatcagac cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg
      541 cgatggaaga aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg
      601 cggcgtacat ccagagaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag
      661 agcgcgtagg ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccac
      721 tgctcggtac gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg
      781 caatgcactt ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg
      841 acaaaggtcc gcaggcggcg ctggcacaga tttccggtct tgatgccaac agtgaggttg
      901 tatccgaggc ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac
      961 cctgcgcccg aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt
     1021 tgaaaaccgt gtgcttgagc gtctgaatgc tggcaaaacc gtacgaagct ttctgatcac
     1081 cgccgtcgag cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga
     1141 cgattacgcg gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga
     1201 tctttctgtg cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga
     1261 agatgcggaa ctgctgatgg cattgcgtga agagctaaat cacgacggca acgagtacgc
     1321 ctttaccgac gacgaaatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc
     1381 accgccgcca caatttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta
     1441 tcaacaggct gtgcgatcaa caatggtcct ttcactgact gagctgattt ccaaaatcag
     1501 cttaaaaaaa gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg
     1561 ccgataaagc cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg
     1621 tattccggac ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg
     1681 gcttgatgcc atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt
     1741 gcccatgatg atatctccgt attaccccgc gccgggaatg cgcggccacc aattttagtt
     1801 taggttcccg gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct
     1861 gctgtttatt atcttttttc tatagatttc ttaattaatc aacgaattgc gtagtttttg
     1921 agcatgttaa tgattacgtt tcagaactat tacaatgtca tcagttgtga acaaagcacc
     1981 tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag
     2041 tcgaaaaaac gaaatcaaac gtctcagcga tcgcctggac gccatccgcc accagcaggc
     2101 agatctgtcg ctggttgaag ccgcagacaa atatgccgag ctggaaaaag agaaagccac
     2161 gctggaagca gagattgctc gcctgcgtga ggttcatagc caaaaactga gtaaagaagc
     2221 acagaagctg atgaagatgc cgtttcagcg cgcaattacc aaaaaagagc aggctgatat
     2281 gggcaagctg aagaaaagtg ttcgcggact ggtggttgtg cacccaatga ccgcgctggg
     2341 ccgtgaaatg ggcctgcagg agatgactgg attctctaag actgcgtttt aagcgcctca
     2401 gacgactggt tcgatcatat caacccgcat ctggcttgcc ttagctggtc aatgcgggga
     2461 ctctcctgtc tactcgccta attaccaaac aattcactcc ctttacgtcg atttaccgct
     2521 ggttgatatt ctgaaaatgc atttttaccg aaatagccac ctataa
//
LOCUS       DAJTNA010000319         2563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTNA010000319 DAJTNA010000000
VERSION     DAJTNA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.325"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..591)
                     /locus_tag="OO161_004149"
     CDS             complement(<1..591)
                     /locus_tag="OO161_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5464977.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPYVAISLDR
                     TEGAKLEFYEAEAKKQAKQFFMDVAAGLCEGNEPLQEKRPIILEAQDVLITYRGKLPG
                     IITCSLKMPPSTLRSEKEASCLQSEYSITVKSAGEEGNKRYFIASAPDKDQEWECNRP
                     SFVVYGDGGKITISENGKLTPPSHQHSEALIEFAIDY"
     gene            complement(596..811)
                     /locus_tag="OO161_004150"
     CDS             complement(596..811)
                     /locus_tag="OO161_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5464978.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAVRGE"
     gene            complement(962..>2563)
                     /locus_tag="OO161_004151"
     CDS             complement(962..>2563)
                     /locus_tag="OO161_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HCR5464979.1"
                     /translation="PDSYDAPHPRIKQLARRNTVTPGGEVCVFNDIIPADHCLHDVQD
                     MSTINHPRADLSKGQYGCVGQGLHIAKKLLPYIPNNAGILLVPCCRGGSAFTQGTEGT
                     FSESTGASQDSARWGVGKPLYQDLLFRTKAALQKNPKNVLLAICWMQGEFDMTNASYA
                     QQPAAFLAMVQQFRADLAGLAAQCHGGSPATVPWICGDTTYAWKQEHGTQYEVVYGAY
                     KGKESQQIYFVPFMTDGSGVNTPTNNPSEDPDIVGSGYYGSASRTNKNWVSSNRPTNF
                     SSWARRGIIPDRMATAILNAAGRTSAFISGKAPEIKPSPGGDTPSGPSADTSVRTISL
                     LPTAGEAAAQGWTIKDGGIQLSGGVFKIAKQSNKAWSLTRPVDDAVSLLTRGGRLSCK
                     FRLSGALTNNQFGLGIYLYTDVALPDVVAMTGTGNPFLMSFFTQTTDGKRNLMHHKKA
                     GNTKLGEFGNYSNDWQTLELVFTAGSATVTPKLNGVAGPAFQAIKDSLAVGLNALTLT
                     DITKNAAYGVEIESLVLEINAPASS"
BASE COUNT          586 a          726 c          592 g          659 t
ORIGIN      
        1 gtaatcaatg gcaaattcaa tgagcgcctc actatgctgg tgcgatggcg gtgttaattt
       61 cccattttct gagatggtta ttttcccgcc atctccgtat acaacaaagg atggccggtt
      121 acactcccat tcctgatctt tatcaggtgc agacgcaata aaataacgtt tatttccttc
      181 ctctccggca cttttaaccg taatggagta ctcagactgc agacaagacg cctctttttc
      241 tgaccgcagt gttgacggcg gcatcttcag agaacaagta attattcccg gtagttttcc
      301 tctgtaggtt atcaacacat cctgcgcctc taaaatgatg gggcgctttt cctgcaacgg
      361 ttcgttccct tcacataacc cggcagcaac atccatgaaa aactgcttcg cctgcttttt
      421 cgcctccgct tcgtaaaact ccagcttggc accttcagta cggtcaagac taatcgccac
      481 atatggcaac aacagtgacg gatacccacc aatttccagt gccacagtaa cagtaatctt
      541 atccgggtaa ttatttatcc ctttaacaac cagttcgtat tttttcttca tcactttact
      601 ctccccgtac agccttacga cggtcctctc tgattttgaa atacaggtta gtcagatatg
      661 tcagcagccc aaacagcaga ctccccagca cgcctattgc cgcccactga gacggggaaa
      721 ccctgtccag caactgcagg aaccagtagc ccgttcccac cgctgacgtg gtgtatgaca
      781 cacctgttgt gattttttcc atctggtaca taccccgtct cccgttatcc ggaagctgac
      841 aacaataaaa aaagccacca gttaagtact gatggctctg ataactcatg caggcatctc
      901 agacgaccca ctgacactac cggtgagttt aacgatacct tccatttgac tggctcactt
      961 tttatgatga tgccggtgca tttatctcca gcaccagact ttctatctca acgccatacg
     1021 ctgcattttt ggtaatatcc gtcagcgtca gtgcatttag tcccactgcc agactgtctt
     1081 ttatggcctg gaatgccggg ccagccactc cattcagttt cggagtaacc gtggcactgc
     1141 cggcggtgaa caccagctcc agcgtctgcc agtcgttact gtaattcccg aactcgccca
     1201 actttgtgtt tccggctttc ttgtgatgca tcagattccg tttgccgtct gtggtctggg
     1261 tgaagaacga catcaggaac gggttaccag tcccggtcat cgccacgacg tcaggtaacg
     1321 ctacatcggt atacagataa attcccagac cgaactgatt gttggtcagt gcgcctgaca
     1381 gtcgaaactt acagctcagt ctgccacccc gtgtcagcag ggagactgcg tcatccaccg
     1441 ggcgcgtcag ggaccaggct ttattgctct gcttggcgat cttaaataca ccacccgaca
     1501 actgaattcc gccgtcctta atggtccagc cctgcgcagc agcctctccg gctgtcggca
     1561 gcagggagat tgtgcgaacg gacgtatctg cagacggacc cgatggcgtg tcgccgccgg
     1621 gcgagggttt gatttccggt gccttaccac tgatgaaggc tgaggtgcgc ccggctgcgt
     1681 tcagaatagc ggttgccata cgatcgggaa taatgccacg acgcgcccat gagctgaaat
     1741 ttgtcgggcg atttgatgat acccagtttt tgttcgttcg ggatgccgaa ccgtaataac
     1801 cagacccgac aatatcagga tcttctgacg ggttgtttgt cggtgtatta actccgctac
     1861 catcggtcat aaagggaaca aaataaatct gctgggattc tttaccttta tatgcaccat
     1921 ataccacttc atattgcgta ccgtgttctt gtttccacgc gtatgtcgtg tcgccacaaa
     1981 tccaggggac tgttgccgga cttccaccgt gacactgcgc cgccagcccg gcaaggtcag
     2041 cacggaactg ctgtaccatt gcaagaaatg ctgctggctg ctgggcgtaa ctggcattcg
     2101 tcatatcgaa ttccccctgc atccagcata tcgccagcaa aacgtttttc gggtttttct
     2161 gcaatgctgc cttcgtgcgg aaaagcagat cctgatataa cggcttaccc actccccagc
     2221 gagccgaatc ctgactggcc cccgtggact cgctgaatgt cccctccgtg ccctgggtga
     2281 atgccgaacc accacgacag catggtacca gcaggatccc cgcgttatta gggatatacg
     2341 gaagcagttt tttggcaata tgtaagccct gtccgacaca gccgtactgc cctttgctca
     2401 ggtcagcccg gggatggtta atcgtactca tatcctgaac atcatgcaga caatggtcag
     2461 caggaatgat gtcgttaaat acgcatactt caccaccggg agtcactgtg ttacgacggg
     2521 ccagttgctt aatgcgcgga tggggcgcat cgtatgaatc cgg
//
LOCUS       DAJTNA010000320         2532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTNA010000320 DAJTNA010000000
VERSION     DAJTNA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.326"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(54..692)
                     /locus_tag="OO161_004152"
     CDS             complement(54..692)
                     /locus_tag="OO161_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5464980.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(702..1100)
                     /locus_tag="OO161_004153"
     CDS             complement(702..1100)
                     /locus_tag="OO161_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5464981.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(1118..1777)
                     /locus_tag="OO161_004154"
     CDS             complement(1118..1777)
                     /locus_tag="OO161_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCR5464982.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(1828..2526)
                     /locus_tag="OO161_004155"
     CDS             complement(1828..2526)
                     /locus_tag="OO161_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCR5464983.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
BASE COUNT          643 a          658 c          560 g          671 t
ORIGIN      
        1 agcggcgatc tggtccagat cccgtcgcac aggaacgttg tgtctcagca tgattagcct
       61 ccccaatgcc cacgggcgct ggaagaacga ccatagccgc cgcgcgaaac ggttgacgct
      121 tttttggtgg taaagccgcc tgaagagtaa gactcttttt tgccagaacc ggagcgccag
      181 gagtaatcac cggaagtgtt gcgccagacg gggcgggaag caaatgagga tccgccgctg
      241 ttatagacga acggctcatc gcgatcttta cgaataaccc ggcttaacaa aaatccagaa
      301 acgaccggga tccaactctg ttcaacattg tcgtagtagc aattttcgta cttagactga
      361 caattctgct gagtcatatt cttcggaaca tcggcataaa atgccgcttt ggcgttgttc
      421 cagccacgcg cgcaaatgtc ggcattatta ccgtcatcaa tacaatcctg cactgtcgaa
      481 taaaacgttc cgtcgccgtc gttatcgaca tcgccactat cgccacaacc tttcaataca
      541 aaaaaagcgg caccgcccat tagcgccaac gttaagtatt tttgtgcgta gcgattgcga
      601 cgcggttcaa aaggattatt cggtctacca atgcgactga tcgctccgtg accaatttta
      661 ctgttatttc ttgatttgcg ttttctggcc atgatcgtcc attaccatgt catacaagct
      721 gcgttaaaaa taccgccagc cagcgcggcg gttcccataa acattcctgc tgcggtattg
      781 tgattaataa ttttttcgct taatgcgggc atataaagtc tgacaccagc aaaaacgagt
      841 aactgaatca ccagtgcaat cccgccccag gcgaaatagt ctggaatact caccgcattg
      901 attgccgcac tggataaggg gatcacgtaa cccaacaatg taccgctgaa tgccagtgac
      961 accgcggtat tattgttttt gattaattgc cattcgttgt gcggtgtaat tttggagtag
     1021 ataaacagaa aaataatcac catagccacg ccaataaaaa aataggcact aaaggcaaga
     1081 agtgaatcca gtatatgcat tgtaaacgtc cttgtaatta accaataata tgaagtgatg
     1141 tcagcgggat atcgacaccc aacgcgcgcg aaaataacca ctctggctcg ccgagatcgt
     1201 taaaagattc ctcaccattt aacagcaaat attcgtaagt atcttcggtt acttgccgct
     1261 ggtagcccat ggtaaaatta tggacctccc atttggcatg gttttgattt tctacttttt
     1321 ccagcatgta gacgggttcg atatttcccg gttcttcgct gttaaaaaat cgctgccagc
     1381 gtttttcctg ccagtttaag gtcattgccc ccatcgtttt ggcgttgatc gcctcgcgcc
     1441 agtgactctc tttactgata ccgtagctct cttcatagac aaagagcttg atatcatcaa
     1501 tgtcatcaat atcttcgccg ccggtggtat tgatttgcag aaattcatcg cctgaagtgt
     1561 agtaacggaa aatctggcta ccgcctccca gatcgatgcg gctgacagcg gctaccgtaa
     1621 attcctcacc cggtagcgca atcagcaatt catcttccag caaacgaaac gctaacgtgt
     1681 cgagcgtaaa gccactattg agatgaagtc ccagcggacc gcgagcgata gcaggcttat
     1741 tatccttgcc aaacagacgc tggaaaaaac cagacatatt ttatccttaa aatagttgcc
     1801 agccttttag cgaggctggc aaaaaaatta ctcgccctgt tggcgttgca gtcttgctaa
     1861 tacatcctgg gctctacttt tattgctacc gccaattccg gcttccgcca gcttttcgtc
     1921 aagatcgcga ccgtctgcga ctttctccaa ctgtgcggca gcatccaggc gagcctgacg
     1981 ttcggcctga cgcgtttgca ggcgttttaa ggattctgcc gctgtcgaaa cgctggaaga
     2041 tgcgccaacg gtagaggttg ttacagcctg ttgtgcacgc tgcatggctt cagtggcttt
     2101 aacgacttcc atttgctgct caaactgagc gatacgctgc gctgtcgctg taactgcttt
     2161 ttccacgcca tcacgggaga cttccagatt cgacaacact tgctcttcag cggtaatgag
     2221 attctcaagg cgcgcgattt cttcagcaac ttcgttaatc aacgacggat taacgttctt
     2281 gcttaacgct tccagcgcac gggcttccag actggctttg cgctcacgta aatctttcag
     2341 cttatcgtga cttaatttca cccgcgccag cagatcaacg cgagattttc cggctttatc
     2401 gagttcagct ttagcgtcac gaatatgctg ttccagcatg cgcacgccct gggtttcttc
     2461 aatggattct tccgcctggg agataaacga tttccccagc gtaaataaac tttttaaaat
     2521 tcccataaat ac
//
LOCUS       DAJTNA010000321         2497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTNA010000321 DAJTNA010000000
VERSION     DAJTNA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.327"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..1322
                     /locus_tag="OO161_004156"
     CDS             <1..1322
                     /locus_tag="OO161_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5464984.1"
                     /translation="LVVVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTM
                     GPGLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLF
                     IFFMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFI
                     PHIDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSL
                     AMGMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSID
                     APLKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYK
                     WLLNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFA
                     CLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANGK"
     gene            complement(1323..2231)
                     /locus_tag="OO161_004157"
     CDS             complement(1323..2231)
                     /locus_tag="OO161_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5464985.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            2331..>2497
                     /locus_tag="OO161_004158"
     CDS             2331..>2497
                     /locus_tag="OO161_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR5464986.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPED"
BASE COUNT          615 a          558 c          621 g          703 t
ORIGIN      
        1 ggctggtggt tgttttttca tgggtgttta tctttgcgct ctatttcaca ccttatgcgc
       61 taattgttgg tcagttaggc tcgaccttca aagatgggaa gggcggggtc agtacctgga
      121 ttaaacacac gatggggccc ggactggctt atctcgccgc gtggacctac tgggtggtgc
      181 atattcccta tctggcacaa aaaccccagg caattctgat tgcgctcggt tgggcgatga
      241 aaggcgacgg ttcgctaatc aaagaatatt cagtcgtagc gttacagggg ttaacgctgg
      301 tgctgtttat cttctttatg tgggttgctt cacgcggtat gaaatcgctg aaaatcgtcg
      361 gttctgtggc agggattgcg atgtttgtta tgtcgctcct gtatgtggcg atggcggtaa
      421 ccgcgcctgc gattactgaa gtgcatattg cgaccacaaa cattacctgg gaaacgttca
      481 ttcctcatat cgactttacc tacattacca ctatttcaat gctggttttc gcggttggcg
      541 gagcagagaa gatttctcct tacgttaatc aaacgcgcaa cccaggaaaa gaatttccaa
      601 aagggatgtt atgcctggcg gtgatggttg cggtttgtgc cattctgggc tcactggcga
      661 tggggatgat gtttgactcg cgtaatatcc cggatgactt aatgactaac ggtcaatatt
      721 acgcctttca gaagctgggc gagtattaca acatgggtaa tactttaatg gtgatttacg
      781 ccattgcgaa taccctggga caagtagctg cgctggtatt ctcgattgat gccccgctta
      841 aagtgctatt aggtgatgct gacagcaaat atattccagc cagtttatgt cgtaccaacg
      901 cttctggtac gcccgttaat ggctattttc tgaccctggt gctggtggca attctgatta
      961 tgctgccgac tctcggcatc ggtgatatga acaatctcta taaatggctg ttgaatctta
     1021 actcggtagt gatgccgctg cgttatttat gggtatttgt tgcatttatt gcagtcgttc
     1081 gcctggcgca gaaatataaa ccagaatatg tctttattcg taataagcct ctggcaatga
     1141 ccgtcgggat ttggtgtttt gcctttaccg cctttgcctg tttgacgggg atcttcccga
     1201 aaatggaagc cttcactgca gagtggacct tccagttggc gctgaatgtt gcaacgccgt
     1261 ttgtgctggt aggattagga ctgattttcc cgctgctggc gcgtaaagcg aatggtaaat
     1321 aattattgtg gtcggtgacg ctgactctgg cagatctgac ccgtttttac ataatcgaga
     1381 aatgcgcgca agccgcagga catatactgg cggtttggat aatagatctg gaagcctgga
     1441 cgctctgtgc tccagtctcc cagtacacat tctaaacgac cagtatcaag gtactccttg
     1501 atctgttcat agagcagata cccgatacca gcacccatca aaacggcctc cagttcagca
     1561 tcaacatcat ccagaatgat atttcctgcg acagcaattt ccagctcctt tgcaaattgc
     1621 cagtggaaag gtttgccgct gggataacga aaaacaacac attgatgatt aagcagatca
     1681 tgaggatggc ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat
     1741 ttcactggcg ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga
     1801 acgccagcgt caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg
     1861 actttaatat caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt
     1921 gcagctaccc gggcggcatt gattttgagc gttcctgtcg gtgtcagacg aaaatcattc
     1981 atctcatcta acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta
     2041 gaacccgctt ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga
     2101 cgttgttcca gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg
     2161 cccgctgcac gaaagctttg attacgggcc acggcaaaga aagtggcaaa gtcagacata
     2221 ttcatccgca ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacaggat
     2281 tatgccgcct gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg
     2341 aacgtattta tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg
     2401 atgcgatcca tcagcgggca atggagcgga aaatacaggt gacggaactc gatttatatc
     2461 ggcgtaattt caacccagtg atgacgccgg aagatga
//
LOCUS       DAJTNA010000322         2450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTNA010000322 DAJTNA010000000
VERSION     DAJTNA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.328"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..167)
                     /locus_tag="OO161_004159"
     CDS             complement(<1..167)
                     /locus_tag="OO161_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5464987.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKM"
     gene            439..741
                     /locus_tag="OO161_004160"
     CDS             439..741
                     /locus_tag="OO161_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5464988.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            732..1214
                     /locus_tag="OO161_004161"
     CDS             732..1214
                     /locus_tag="OO161_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5464989.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            1282..1967
                     /locus_tag="OO161_004162"
                     /pseudo
     CDS             1282..1967
                     /locus_tag="OO161_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1984..2450
                     /locus_tag="OO161_004163"
                     /pseudo
     CDS             1984..2450
                     /locus_tag="OO161_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          636 a          572 c          632 g          610 t
ORIGIN      
        1 tacattttgt ggaaggtttc ccagaaacag accgtgcagg agggatgacc ggtgataaag
       61 tctgcaaata cggacttata agggtgatta ttggctctgg cgacggcttt cagaacctct
      121 ttcaccattc tggtgtggac tttctggtgc tccaggttgt gtgacatggg aactcattct
      181 ggatggttac tctgaaagct catattctgc cacaccccga tttgcagccg ccaggctgcc
      241 gtggttcagg catttcatta tcaaaatcac attaaacggc cgtaatcaga catgatttgt
      301 gcgccgacac agatcatcgt cacaatgttc aggtctctgc cattgttttt atcaggggat
      361 cgcttttccc gtaagtgatt ctgtatagcg ttctgttact atttgcgata acattaattg
      421 catagcaaat tgttaccaat ggaggttttt atgtctacag ctgcaagcgt ccgtaaaaca
      481 ccgcgagaac atcagattaa catccgggca actgatgagg aacgagccgt tattgattat
      541 gctgcgagct tggtcaataa gaaccgtacc gatttcatca tggaactggc gtaccaggaa
      601 gctaaaaaca ttatcctgga tcaacgttta tttgtgctgg ataacgaacg ttatgattcg
      661 tttattacgc aactggaagc tcctgtgcag aatgcagaag ggcgtgaacg tctgatggcg
      721 gtaaagcctg aatggaaata aatgtcaccg cgccagcatt gttgacggat gagcatatac
      781 ttcagccatt tgactgtgga aatgaggtgc taagtaactg gttacgtggt cgggctatga
      841 aaaaccagat gctcaatgct tctcgcacgt tcgttatttg cttggaggat actttacgtg
      901 ttgtgggata ctactcatta gctactggtt ccgtcactca tgccgagctt ggtcgcagtt
      961 tgcggcataa catgcccaat cctgtccctg ttgttctgtt agggcgtctg gctgttgatg
     1021 tttgtaccca ggggcatggt ttcggtaaat ggctactaag tgatgccatt caccgggttg
     1081 tcaatctggc tgatcaggtt ggcattaagg ctgttatggt acatgcaatt gatgatgacg
     1141 ccagagcatt ttatgagcgt tttggttttg ttcagtcagt tgtggcacct aataccttgt
     1201 tttataaagt ctgagtctga ggtagcctga gtttaacgga cactccttcc tgaaatagaa
     1261 tggcatcaga aggagccaat aatgagcaga aaaacccaac gttactctac ggagtttaaa
     1321 gccgaagccg tcaaaacggt tcctgaaaat cagctttcga tcagtgaagg cgcttcccga
     1381 ttatctgttc cttaaggcac tttaggacaa tgggttaccg ccgcaagaaa agggctcaat
     1441 acaccaggca cccgcacagt ggctgagctg gaatctgaag ttatgcaact gcgtaaggct
     1501 ttaaatgaag cacgacttga gcgagatata ttaaaaaaag caacagcgta ttttgcccag
     1561 gagtcgctga aaaatacgcg ttagtcgaac aatggcgaca acaattccct gttgaagcga
     1621 tgtgtcgggt acctggtgta tccaggagcg gttattacga ccgggtacag catgccccct
     1681 cagacagaaa acaaagtgat gagcggctaa aactggagat taaggtggca catatccgca
     1741 ctcgcgaaac atatggaacc cggcggctcc agacggagct ggcagataac ggaattatcg
     1801 tcggtcgtga cagactggca cgtcttcgta aggagctgag gctgcactgt aagcagaaac
     1861 gcaagttcag ggcgaccaca aactctgacc acaatctgcc ggttacgccg aatctgctga
     1921 atcagaactt tacgcccaca gcacctaatc aggtctgggt cgcagattca gttgtccagg
     1981 ccttccgaaa tcaaccgact gaaggcgctg gtcgcgaaac tgcagcgtat gcagttcggt
     2041 aaaagctcag aaaaacttcg cgcaaaaacc gaacggcgga tacaggaagc acaggagcga
     2101 atcagcgcac ttcaggagga aatggcggaa acactgggtg agcaatatga cccggtactg
     2161 ccatcttccc tgcgccagtc ttcagcccgt aaaccgttac cagcctcact tccccgtgcc
     2221 ccccgggtca tccggccgga agaggaatgc tgtccggcct gtggtggtga actcagtcca
     2281 ctgggatgtg atgtgtcaga gcagctggag cttatcagca gcgcctttaa ggttatcgaa
     2341 aaacaacgcc ctaaactggc ctgtcgccgg tgcgaccata tcgtgcaggc accggtgcct
     2401 tcaaaaccca ttgcacgcag ttatgccgga gcggggcttc tggcccatgt
//
LOCUS       DAJTNA010000323         2392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTNA010000323 DAJTNA010000000
VERSION     DAJTNA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.329"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(195..269)
                     /locus_tag="OO161_004164"
     tRNA            complement(195..269)
                     /locus_tag="OO161_004164"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Arg,seq:cct)
     gene            complement(345..1277)
                     /locus_tag="OO161_004165"
     CDS             complement(345..1277)
                     /locus_tag="OO161_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5464990.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEMDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKKPA"
     gene            1571..2326
                     /gene="mlaA"
                     /locus_tag="OO161_004166"
     CDS             1571..2326
                     /gene="mlaA"
                     /locus_tag="OO161_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5464991.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          576 a          580 c          591 g          645 t
ORIGIN      
        1 taatggctac atacggcggt cagtttacgc ttacggtaaa aatccggtgg gtaaaattat
       61 tttatccact ttttacccgt catggagtgc ggctgtcaac gatctgacgc gaaccatgac
      121 gaaccgtgaa tatacggaag gcttgatatt caggggattt tgcggactgg tacggatggg
      181 agcgaactga taaatggtgt cccctgcagg aatcgaacct gcaattagcc cttaggaggg
      241 gctcgttata tccatttaac taagaggaca atgcggcatg agtatacccg ctaatggact
      301 gcggagtaag tacactgccg ctcgattgct taaaccctcg ccatttatgc tggtttttta
      361 tcatttttct taatgttttc cgcacgttct gctttttggc gtgcttctgc tttgcgcttg
      421 ttgctcatgt cgttacggat ctgtgcatga ctcattaacg cgaagataaa ggtgccgccg
      481 cagatattcc ccgctaaagt aggtagtgcg aagggccaaa tgaaatcgct ccagtgcagc
      541 gtgccgttaa acaccagata gaggatttct acagaaccga caacgatgtg ggtggtgtca
      601 cccagtgcaa taagccaggt catcaatata atcaccacaa tctttgccgc acccgctgca
      661 ggaaacatcc aaaccatagt ggcgatcagc cagccggaaa tgatcgcgtt ggcaaacatc
      721 tcgctggggg tgttcttcat cacatccatg ccgattttga caaatgcatc gtgagtttct
      781 tcattgaaga taggcatata ttcaaatgcc catgcagcaa tacctgtccc gagaatatta
      841 cccagcagca cgacgcccca taatcgcata agtaagccga cgttgctcat tgtcggtttt
      901 tgcatgacgg gtagtaccgc agtcacggtg ttttcggtaa ataattgctg gcgggccata
      961 atgacgataa taaaaccaaa ggtataaccg agattctcca acaagaagct gcccggcacg
     1021 ccttccagtt cgacatgaaa tatccctttt gccagtaacg aagcgcccat cgacagaccc
     1081 gccgcaatgg ctgaccacag tagtgccatt gcgtcgcgtt ccagctcttt ttcaccatcc
     1141 tggcggatat gttcatgaat tgccatcgcc cgggagggga gtcgatcttc atccatttct
     1201 atttttttgc cgcgctcttt ttcttcgctc tcaacttcaa tttcgtcgct gtgttgatca
     1261 attttgtcgt tgtccatggt ctcttctctt gaattagcac gtatagctaa agcgtagcgg
     1321 cttttttgct cgcaactggc gggagttact ctgaaaatgt agaaaaggcc gcgtttgcct
     1381 ttttctgttt ctatagaatc aagtagccta cagggcggcg gttaccaggc tatgatcaaa
     1441 tcagcaaatc agggcgtctg gacatcagtt gacgtgctgt tacaatcgcc cacacctaaa
     1501 caggcggata cggtatcgtt ccgtcatgga tggcaaactg cataagccat aaaaaaacag
     1561 ggagacattt atgaagcttc gcctgtcggc gcttgctctg ggaactacgc ttctggtggg
     1621 gtgtgcgagt tccggtacag atcagcaagg gcgttctgac ccgttagaag ggttcaaccg
     1681 caccatgtac aacttcaact tcaatgtatt agacccgtat attgttcgac cggtcgctgt
     1741 cgcctggcgt gattatgttc cgcaaccggc gcgtaacggt ttgagcaact ttaccggcaa
     1801 ccttgaagaa cctgcggtga tggttaacta cttcttgcag ggcgaccctt atcaggggat
     1861 ggtccacttt acccgctttt tcctgaacac cattttgggg atgggcggct ttattgatgt
     1921 tgcagggatg gcgaacccga aactgcaacg gactgaacct caccgcttcg gtagtacgct
     1981 tggtcattat ggcgtgggtt atgggcctta cgttcagtta ccgttctacg gtagcttcac
     2041 gctgcgtgat gacggtggtg atatggcgga tggtctttac ccggttcttt cctggctgac
     2101 ctggccgatg tctgtgggta aatggacgct tgaagggatc gaaacccgcg ctcagctgct
     2161 ggattccgat ggtctgctgc gtcagtcgtc cgatccttat attatggtgc gcgaagcgta
     2221 cttccagcgt catgatttca tcgctaatgg cggcgaactc aaaccgcagg aaaatccgaa
     2281 cgcacaagcg attcaggatg atttaaaaga tattgattct gaataagaaa caaataaaaa
     2341 aggtgagtcg caaaactcac ctgttttgtt gttatccctc aattaccaca ag
//
LOCUS       DAJTNA010000324         2384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTNA010000324 DAJTNA010000000
VERSION     DAJTNA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.330"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            437..2089
                     /locus_tag="OO161_004167"
     CDS             437..2089
                     /locus_tag="OO161_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5464992.1"
                     /translation="MISLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO161_004168"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO161_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          740 a          448 c          555 g          641 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaactg aagccttgca aacggggatg
      421 cgttcataca ggtacaatga tatcgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaacgc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccggg aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgg
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccgtatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatc
//
LOCUS       DAJTNA010000325         2283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTNA010000325 DAJTNA010000000
VERSION     DAJTNA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.331"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            484..627
                     /locus_tag="OO161_004169"
                     /pseudo
     CDS             484..627
                     /locus_tag="OO161_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            774..1042
                     /locus_tag="OO161_004170"
                     /pseudo
     CDS             774..1042
                     /locus_tag="OO161_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1435..>2283
                     /locus_tag="OO161_004171"
     CDS             1435..>2283
                     /locus_tag="OO161_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5464993.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSSTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTEPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          763 a          490 c          385 g          645 t
ORIGIN      
        1 ctctcgtctg gggaatatct ccccggcagc cttcaggata aaatattatc agatgactgc
       61 ttagaaaaag aacaaatggt ggtgtccgct attgccagta cacctcaagc ttcttaccat
      121 atttgaaata aagctcaata gcgcaaattt tttgttcttc agtaaacatg tttcatcttc
      181 tgagagtcca ggaaatcgtc cgcatcccca ttaccgtctt atatcactgg cgctgacatg
      241 tgctcattga tgaagtgaat ctgaatgaca tgttttgcca tatcaacgcc aaccggtgta
      301 tatttcattt gtggagtctc cagtctgagg acgctttatg catcccgtat tgggcagtat
      361 gatgccggaa agctgcgagg ctccacacct gccagtttca cccttttaac catcatacta
      421 ctccgtgttt aattgtgtgg gatgcgttca ttacatttta acagtgtcac cccgtcggcc
      481 acgaaagata aacaggtgac cagaaaaggg atcttcatcc agcacatgct atatcttgaa
      541 cgtaacaacc tgaccctccg cacccgcatc aagcggctgg cacgtaaaac aatctgcttc
      601 tcgcgttccg tggaaatcca cgaaaagtca tcggctcctt cattgaaaaa cacatattct
      661 actgattgga agcgccaccc gaaaaagtat cgttttttta cagtaaactt catttagccg
      721 actataatgt aaaaaaataa attttttacc aaaatataac ataaggaagt aatatgctta
      781 cacaaactat attcccttgt cttcccaaaa aacaagaaaa tatcatccct gaagtatcaa
      841 atactatatt agtatcttct actgttacta cagatggatg tactgttttt aataaacaaa
      901 aacagccata tacgaattgc aaatacctgc taccaaccga acgaaaaagt tgaaattaac
      961 agctacagaa atacaatggt tgacaaagat aaatgaagcc agaattgatg aaaaacaaag
     1021 ccaacggtat tctgatttct gaagttaatt tcagactcgg ttaagctcca ttttcaaaaa
     1081 aaaatatatc agggtttgtt actaataact tctgaaaaca gaaaaaaaat agagcagtaa
     1141 ttctatttca gagaggagta actccatcta tcctcgatac gtactgaacg ttatcgtgat
     1201 atattcatca tgctttcttc accattattc gagtacaggg agagacgcaa tgaccttaaa
     1261 taccttttta tggctgaatt accattctca ggaataacag cagactcctt cccatagacg
     1321 gcttaggggg aaagcaatac ccggagaaag ggtacaaagc aaactgtttt aaaattaata
     1381 agctatattc tatttcacta gaattcttat cgttgataag aaattttagt tataatgatt
     1441 aaatcaacca atatacaggc aatcggttct ggtattatgc atcaaataaa caatatatac
     1501 tcgtcaactc catttccttt acctatggaa ctgactccat cttgtaatga attttattta
     1561 aaagcctgga gtgaatggga aaagaacggt accccaggcg agcaacgcaa tatcgccttc
     1621 aataggctaa aaatatgttt gcaaaatcaa gaggcagaat taaatttatc tgagttagat
     1681 ttaaaaacat taccagattt accgcctcag ataacaacac tagaaataag aaaaaaccta
     1741 ttaacacatc tccctgattt accaccaatg cttaaggtaa tacatgctca atttaatcaa
     1801 ctggaaagct tacctgcctt acccgagacg ttagaagagc ttaatgcggg tgataacaag
     1861 ataaaagaat taccatttct tcctgaaaat ctaactcatt tacgggttca taataaccga
     1921 ttgcatattc tgccactatt gccaccggaa ctaaaattac tggtagtttc tggaaacaga
     1981 ttagacagca ttcccccctt tccagataag cttgaagggc tggctctggc taataatttt
     2041 atagaacaac taccggaatt accttttagt atgaacaggg ctgtgctaat gaataataat
     2101 ctgacaacac ttccggaaag tgtcctgaga ttagctcaga atgccttcgt aaatgttgca
     2161 ggtaatccac tgtctggcca taccatgcgt acactacaac aaataaccac cgaaccagat
     2221 tattctggtc ctcgaatatt tttctctatg ggaaattctg ccacaatttc cgctccagaa
     2281 cac
//
LOCUS       DAJTNA010000326         2275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTNA010000326 DAJTNA010000000
VERSION     DAJTNA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.332"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..349)
                     /locus_tag="OO161_004172"
     CDS             complement(<1..349)
                     /locus_tag="OO161_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR5464994.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRN"
     gene            591..884
                     /locus_tag="OO161_004173"
     CDS             591..884
                     /locus_tag="OO161_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5464995.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            956..1615
                     /locus_tag="OO161_004174"
     CDS             956..1615
                     /locus_tag="OO161_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5464996.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1707..2153
                     /locus_tag="OO161_004175"
     CDS             1707..2153
                     /locus_tag="OO161_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5464997.1"
                     /translation="MNDVPFWQSKTLAEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          592 a          492 c          587 g          604 t
ORIGIN      
        1 cgtttcgggt ttgcagcact cgcagctcat atttgccaga agcagggagt gagtattgtc
       61 cgtgactatc gagttctggg gaatatctgg ataaatcaac agaatcatcg attcctggcc
      121 cgaagaggta agtatcagcg ccttcattag agatactcac atgtactttc tgaccttttt
      181 tggcatagaa ggtatatgta tcgtaatcgt atccctttat ttcgccggag tactgagcgc
      241 tgctatggcc ttttctgaac tcaacattga cattcttacc cgcagcaaag ctcgtggagg
      301 ttagcagtgc aaacagcaat acagccttac tgatgctctt gattttcatt ggtagtctcc
      361 tgttcagaat gagtacgatg aactgttata atataacaat ccctaacggg agaattccag
      421 tggcttttct gcgttcggta aaatcccggc gcggcatttt ttgccggggg gattacttat
      481 aaaggggcat ggactcaaca aaccgcagaa atctacagct ggctggcttg tggcgcgggt
      541 ttcattgatg ttagcttatg ccaaaacctg gcatcttaaa gagtaaaagt atgttttgtg
      601 tgatttatcg aagcagcaag cgtgaccaga cctatttata tgtcgaaaaa aaagacgatt
      661 tttcgcgtgt tcctgaggaa ctgatgaaag gttttggtca gcctcagtta gcgatgattc
      721 tgccgttgga tgggcggaag aaactggtca atgctgatat tgaaaaggtt aaactggcat
      781 taaccgagca aggttactat ttgcagttac cgccaccacc cgaagatttg ctgaagcaac
      841 atctttccgt catggggcag aaaacagacg acacgaacaa ataaccgata tccggcggtg
      901 gcattatctt tgtcggcgcg ggttttcata tccacgataa ggagagggga acgttatgta
      961 tcaacatcac aactggcaag gtgcgctgct ggattatccg gtgagtaaag tagtctgtgt
     1021 tggcagtaac tatgccaaac atattaaaga gatgggcagc gcagtgcccg aagagccagt
     1081 gctgtttatt aaaccagaaa cggcactgtg cgatctgcgg cagccattgg cgatcccatc
     1141 cgatttcggt tcagttcatc atgaagtcga actggcggtg ttgattggtg cgacgctgcg
     1201 tcaggcaacg gaagagcatg tccgcaaagc cattgccggt tacggcgtgg cgcttgatct
     1261 gacattgcgt gatgttcagg gaaaaatgaa gaaagccggg cagccgtggg aaaaggctaa
     1321 agcgtttgat aactcttgtc cgctttccgg gtttatcccc gcggcggaat ttaccggcga
     1381 tccacaaaat acaacgctgg gcctgagcgt aaacggcgaa caacgccagc aaggtacgac
     1441 tgcggacatg atccataaaa tcgttccgtt gatcgcttat atgagcaagt tttttaccct
     1501 caaggccggt gacgttgtgt tgacaggcac gcctgatggc gtcggcccgt tgcaaagcgg
     1561 tgatgagctg acagtcactt tcgatgggca ttctttgaca actcgcgttt tgtaatactt
     1621 tttgccgcct gaaagcggcg gcaaaacttg catcgctgtg ccagactggt tataaggtgc
     1681 gttttaacgt aatggcggaa cacctgatga acgatgtacc tttctggcaa agtaaaaccc
     1741 tggccgaaat gagcgatgcg gaatgggagt cgttgtgtga tggttgcggt cagtgttgcc
     1801 tgcataaact gatggatgaa gacaccgacg aaatctactt cactaacgtc gcctgtcgcc
     1861 agctcaatat taaaacctgt caatgtcgga actacgaacg tcgtttcgag tttgaacccg
     1921 actgcattaa attaacccgt gaaaatctgc caacattcga atggctgcca atgacctgcg
     1981 cttatcgttt gctggcggaa ggtaaagatt tacctgtgtg gcatccgcta cttactggtt
     2041 cgaaagcggc aatgcatggt gaacgtatct ctgtgcgtca tatcgcagtg aaagaatcag
     2101 aagtgattga ctggcaggat catatcttaa ataaacctga ctgggcgcag tgataaattt
     2161 aatagatgtt gatgggggct ttcttaatat acgtctggat gctcgccatt tcgtagccct
     2221 gtttttgaaa gcgctctgtc ataatgagag ttacccgata ccttattatc aacag
//
LOCUS       DAJTNA010000327         2262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTNA010000327 DAJTNA010000000
VERSION     DAJTNA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.333"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..107
                     /locus_tag="OO161_004176"
     CDS             <1..107
                     /locus_tag="OO161_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR5464998.1"
                     /translation="KNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(134..1033)
                     /locus_tag="OO161_004177"
     CDS             complement(134..1033)
                     /locus_tag="OO161_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5464999.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(1144..>2262)
                     /locus_tag="OO161_004178"
     CDS             complement(1144..>2262)
                     /locus_tag="OO161_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5465000.1"
                     /translation="AFLTGIAGALQTPTLSIFLADELKARPIMVGFFFTGSAIMGILV
                     SQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNRNYFILLSTGVLLSSFASTANPQ
                     MFALAREHADRTGRETVMFSTFLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAF
                     VVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRDTRLLFVVCSMMWAANNLYMINM
                     PLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYYMKRIGKRLLMLIAIVSGMCFYA
                     SVLMATTPAVELELQILNAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWII
                     AGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIKDI"
BASE COUNT          689 a          547 c          525 g          501 t
ORIGIN      
        1 ataagaatgc agaaaacgtg aaggaatttc tgcaatctta tcaatcaccc gaagtcgcta
       61 aagcggcaga aaccattttt aacggtggcg cggtgcctgg ctggtaattc tcactcatgc
      121 cgcgccggac gatttatttt tctggccggc gacgggaatc cattgaaatc aaaatcaccg
      181 atgacagaat cagcagcgtt cccagccagt ccggtaatgt gaacgtgatc cccagcaaca
      241 gcaaagagag tagcgcgcta cttaatggtt ctgcacagct caaaatgctg gcttttggac
      301 cgccaattaa ttgtgctcct ttcaggtaca gactaaatgt cagggacgta ccaatgacca
      361 ccaaataaaa aaacgccaga atcaaactgc cgttaacgac aaagtttgtt ccttgtctgg
      421 cataaaaagg caacagaatc agaccgccaa tcagcatact ccagccgacg actggtaacg
      481 tgccatagcg ggcaattagc gttgagggat aggtggtata gaatgcagca gcaaatgccg
      541 aggcaatgcc ccagaacaac gcggcaggag agagcgataa tgacgtcgga ttaccgtgtg
      601 tcaccaataa aaaagtcccg accagcgatg tcaaaatagc gcagaaaacc agaatgcccg
      661 ggcgcgattt acgcaccagt gagaaccagg cgacgataat cgtcggtgag aggaattgca
      721 gcaccgttgc cgtggctgcg ttcgattttt cgatggttag caaaaaagtg agctgtacag
      781 ttagcgcgcc aaccacggaa aaaatcagca ggctaatggc atctttatga ttgttaataa
      841 tagaaaagat tttatcgcca tgaacaaatg acagcgtcag tagaatcaaa ccggcgaata
      901 tcaaacgcgt catagtcaaa aactgcgacg acatctggct ttgctccatg atgtattgcg
      961 cgcagacccc tgaacttccc cacaacacgg cggcaatcag aacgttcagc atcccctttc
     1021 tggtggaacc catttttccc tctgcatact gttttgtcgt ttatgtatgc aggcatgata
     1081 gcaaaaatgg cgaggatggc tctgtttgcg ccacgccgat ttttcttagc ccagactggt
     1141 gagctaaata tctttaataa acagcaggca aatcatcgca atacccaaca tccctatcgc
     1201 cagccagaac aacgcatggt agctccagat ttcaaccata attccgtcaa cagagccggc
     1261 gataatccag ccgacgcgtg aagtatttgc atataacgtt gtcgcagagc ctattttttc
     1321 aggcatcaag tcctgaaaat aaagcatgcc gataccacag agaataccaa ggaagatggc
     1381 atttagaatt tgcaattcca gctcaaccgc cggagtcgtc gccatgagta cgctggcgta
     1441 aaaacacata ccactcacga tagcaatgag cattaatagt cgcttgccaa tacgtttcat
     1501 gtaatagcct gcgattaaca tcatcggaat ttccagaccg gcagcgatac caatcatttc
     1561 tccagccagt ttatcggtta gatgcagttc atcaataata aatagcggca tatttatcat
     1621 gtagagatta ttcgccgccc acatcattga acagaccaca aaaagtagcc gcgtatcccg
     1681 cttcctatgg gtggagggta aaatttctac gggttgggta acgacaggaa tatttctttg
     1741 tattgatggc aaaaacaacc agactatcag cccgcaaaca acaaatgcga tggcagcggt
     1801 gagatacatc actttaaaac taaaccccat tgccagttca taagcgagcg gtggcccgat
     1861 aacccaggca agcgagatct gagcacgtaa aaatgtactg aacatgaccg tctcacggcc
     1921 tgttctgtcg gcgtgttcac gggcgagggc gaacatttgc gggtttgcgg tagaagcaaa
     1981 actactcaga agtacgcccg tagagaggag aatgaagtag ttgcgattcc acgcaaaaag
     2041 cgtgcaggcc agcactccaa ataagcaaca tagcagaatc agtaatttac ggtcgccttg
     2101 tttatcggag tgccttgcca gaaattgact gaccagaatt cccataatag cgctaccggt
     2161 gaagaaaaaa cctaccatta taggacgggc tttcagttca tctgcgagga atatacttag
     2221 ggtaggagtc tgaagagcgc ccgcaatacc cgtcagaaag gc
//
LOCUS       DAJTNA010000328         2254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTNA010000328 DAJTNA010000000
VERSION     DAJTNA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.334"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..680)
                     /locus_tag="OO161_004179"
     CDS             complement(<1..680)
                     /locus_tag="OO161_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR5465001.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVLLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLREL"
     gene            complement(728..2083)
                     /locus_tag="OO161_004180"
     CDS             complement(728..2083)
                     /locus_tag="OO161_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5465002.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNIDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(2087..>2254)
                     /gene="gatB"
                     /locus_tag="OO161_004181"
     CDS             complement(2087..>2254)
                     /gene="gatB"
                     /locus_tag="OO161_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5465003.1"
                     /translation="RVNEIETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGIGIEA
                     LQNKILTILQG"
BASE COUNT          668 a          617 c          469 g          500 t
ORIGIN      
        1 agttcgcgta aaacgctctg catttgcggc gcgctcattt cgctactgtt aaatgtttgc
       61 atcgcaccga aagattttgc cagtgccagt ttttctgagc taatgtcgat agccgtcaca
      121 ctctttgctc ccagcgcgac agcgcactga atagccagca ggccaatggt tccggcacca
      181 ataataataa cgtttttatt ctcacaacct tgcgctaaat gaaaagcatg cagaccaacg
      241 gtaatcggct caataaaagc cccatcctca ataggcatat ccgtgggtag agcaaagaca
      301 ttttttcgct taacgacaat atattcagca aatccaccat cacgccgcga gccaataaaa
      361 tcatattttg cgcactggga ataaaaccct ttcagacact ctggacaagt aaaacagggt
      421 aataacagca cacaggcaac cgcatcgcca gggtgtaaat catcaacacc ggatcccacc
      481 gcatcaatat agccgctaaa ttcatggcct aacgttattg gataataatg tgcaccattt
      541 ttaaatatcc tgggtaaatc ggaaccacat aagcccgagc tggcaatttt tacccgcacc
      601 tcatcctgat gtttaatttc aggaatgacg ctttctgcaa cgcgcacgat accatcagta
      661 tcattcacca ctgatttcat aaaaactcct gattgttaag ggggataacc tccccctcag
      721 gtaaaaatta ttctgcgaga acgactttct cttgtttaat aaagccacgc gctctacgcc
      781 aggtcatgaa aataccggtc agataaattg caccgataat aatgaaaccg ggaatatttt
      841 gcggggagaa aacctgaatc agtaaccagg taatgggaga accgccctga tccattgaag
      901 ccaccatacc cccggctttt aacgccccag cattagccgc cagttgggtg tgcaaaccaa
      961 tagtttgcgt cgcgatccac agagtgatgc tcatgatgat gacacccgag attaaggtgc
     1021 ggaacagatt tccacgatgc acggcgactg ccatcgccac aaagaagccg atggtggcaa
     1081 gatcgccaaa cggcagcacc tgattacccg gaacacaaac agcaattaaa atggtgagcg
     1141 ggataaaaat caggcttgcc gataccaccg ccgtatgccc cagcagtaat gcgggatcca
     1201 ggccaatcag gaactcctga ccgccgaact tcgcctgtaa acgactacgg gcctgcttag
     1261 cgatgggcgt taaaccatcc atgatgggtt taatcacccg tggcattagc agcatcactg
     1321 ccgccgtttt taccgccagc tgcaatacac ctttgacatc gtaacccgcg aggatgccga
     1381 taatcagccc catcacaaaa cccacggtga caggctcgcc aaatggacca aatttgcgct
     1441 gaatatcgtc ggcgctaaat ttaattcggt taacgcctgg gattttctcg atgatagcat
     1501 cgaccagcac cgcaatcggc cccatatacg ccgacgtacc gtgcggaata gcaatacctt
     1561 ccagctcaaa gaaatttcgg gtatcgcggg caaaccagtc gccgagctta taaacaaacg
     1621 ccgcgtgaat taccacacct gccatcccta tcatccatga accagttgcc agatgcagca
     1681 acgcgccggt gaaggtcata tgccagatat tccagatatc aatatttacc acccgcgtca
     1741 tacgggtcag taacatcgcc acgttaacca gaatcgcaat cggaatcgcc accagcgcaa
     1801 tttgcgacgc ccaggtcatt ggtgaagagc ccggccagcc gacatcgacc acatgcagat
     1861 tcaggtcgaa attttccgcc atcgctttcg ccgccggacc aatggaatcc agcattaagc
     1921 caatcacaag gccaatgcca acaaagccaa tcccgatatg cagacccgct ttaaagcaat
     1981 cgcctgcctt catgcctaat attttagaaa aaataatgat gacaatcgga agcatcaccg
     2041 tagggccgag gtcgagaata taacgcatga cttctgaaaa cataggtcac ccctgtaaga
     2101 tagtcagaat tttattttgt aatgcttcga taccgatacc agaaataaaa ggcatgccgt
     2161 gaactaacgg aatatcgcca aaactacgat ccactttggc agtggtgcat atcaaatgca
     2221 caccatccat ataggtttct atttcattaa cccg
//
LOCUS       DAJTNA010000329         2231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTNA010000329 DAJTNA010000000
VERSION     DAJTNA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.335"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(15..326)
                     /locus_tag="OO161_004182"
     CDS             complement(15..326)
                     /locus_tag="OO161_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5465004.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            489..585
                     /gene="ffs"
                     /locus_tag="OO161_004183"
     ncRNA           489..585
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO161_004183"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            705..1058
                     /locus_tag="OO161_004184"
     CDS             705..1058
                     /locus_tag="OO161_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5465005.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1100..>2231)
                     /gene="pdeB"
                     /locus_tag="OO161_004185"
     CDS             complement(1100..>2231)
                     /gene="pdeB"
                     /locus_tag="OO161_004185"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5465006.1"
                     /translation="IRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGNAHNVV
                     ITSSDEIAQGIITRLQKTPGEHIENNGIIYDILPFPEMNISIITWASTKMLQKGWHRQ
                     VFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDAIENRDICVHYQPIVSLANGKIV
                     GAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMGDWLRQHPQQHI
                     SINLESTVLTPEKIPQLLREMINHYQVNPRQIALELTEREFADPKTSAPIISRYREAG
                     HEIYLDDFGTGYSSLSYLQDLNVDILKIDKSFVDALEYKNVTPHIIEMAKTLKLKMVA
                     EGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDFLRWAEQHL"
BASE COUNT          541 a          548 c          582 g          560 t
ORIGIN      
        1 ccgcccggag agggttagta gttccagcga taacgctgca agtcgatttg cccgcttccc
       61 gataccatca caccttctgc cagtaatgcc tgtcgctgac gctgtaaatc cggtccggtt
      121 agcgaaattg tgccgtggcg attaaccacc cggtgccagg gtaaggtgct gccttcaggg
      181 agacgcttta acacaccgcc cacctggcgc gcggcgcggg gcgatcccgc cagtttcgcc
      241 acatcaccgt aagtggtgac atagccttcg ggaatagcgg cgacgatttg ccagacgcgt
      301 tggggaaatg aatcttcttt ttccatcttt tcttcctgag gtaatttttc agcataatct
      361 ggaaaaacgc ccgagtgaag tcgcattgcg caagaaacca gcatctggca cgcgatgggt
      421 tgcaattagc cggggcagca gtgataatgc gcctgcgcgt tggttctcaa cgctctcaat
      481 gggggctctg ttggttctcc cgcaacgcta ctctgtttac caggtcaggt ccggaaggaa
      541 gcagccaagg cagatgacgc gtgtgccggg atgtagctgg cagggccccc acccatttct
      601 gcctcccacc gtttcgtcaa aaaaaaccaa catggctaaa ctttaaccac aactgacgtt
      661 gcaagaattg tctggctgcg cagtacgctt cggaggtatg tctgatgaag tatgttgatg
      721 gttttgtggt tgccgttcct gccgataaaa aggatgccta tcgggaaatg gccgctaagg
      781 ctgcgccatt gtttaaagag tttggcgcgc ttcgtattgt cgaatgctgg gccagcgatg
      841 taccggatgg caaagtgacc gattttcgta tggcggtgaa agcggaagag aatgaagagg
      901 ttgtctttag ctggattgaa tacccttcaa aagaggtccg cgacgctgct aatcaaaaga
      961 tgatgtcgga cccacggatg aaagagttcg gcgagtccat gccgtttgac ggcaagcgaa
     1021 tgatctatgg cggattcgag tcaatcatcg acgaataggt agcgtgacgg gctgtgcggc
     1081 gtgccgtcag cccaaaaatt cacaaatgtt gctcggccca gcgtaagaaa tcttctttcg
     1141 gtaatgcctt gctgtagagc cagccctgac cgtagtgcac gccatgctgg cgtagccact
     1201 cttcttgttt actggtttcg attccctccg ctaccatttt cagtttcagt gtttttgcca
     1261 tttcgatgat atgcggcgtg acatttttat attccagcgc atcaacgaaa gatttatcga
     1321 tcttcagaat gtcgacattc agatcctgta aataacttaa acttgaatat cccgtaccaa
     1381 aatcatcaag ataaatttca tggcccgcct cccggtagcg agaaattatc ggggcgctgg
     1441 ttttcggatc ggcaaactcg cgttcagtga gttcaagcgc gatctgtctg ggattaacct
     1501 gatagtgatt gatcatttca cgcagcaatt gcgggatttt ttccggggtg agcacggtgg
     1561 attcaagatt gatcgaaata tgctgctgtg gatgctgacg cagccagtcg cccatatctt
     1621 caaagacgct tcttataatc agtagcgtca atggctcaga aaggcctgtt tgctgtgcca
     1681 gcggaataaa actatctggt gacaaccaac taccgtctgt ctgcggccag cgcgccagtg
     1741 cctcagcacc gacaattttg ccattggcta aggagacaat cggctgatag tgcacgcaaa
     1801 tatcacgatt ttcgatagca tcctgcagcc gatgatgcgg tgactggata cggcgcaaaa
     1861 tacgcagcac aaacatcgct gccagcaggc caatcaccaa cccgagcggt aaccaaataa
     1921 agacttgccg atgccagcct ttctgcaaca ttttcgttga agcccaggtg atgatcgaaa
     1981 tattcatctc cgggaagggc aggatatcgt aaatgattcc attattttcg atatgctcac
     2041 cgggtgtttt ttgtagcctg gtaataattc cctgagcaat ttcatcgctg ctggttatga
     2101 caacgttatg ggcattgcca ataatggcgg catcaatttg ccatgagcta tagggaatga
     2161 catcaataaa ggaagcgggg tcgatcatga cgacataatg tgccgttccc atggcgacca
     2221 tgtaacggat a
//
LOCUS       DAJTNA010000330         2215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTNA010000330 DAJTNA010000000
VERSION     DAJTNA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.336"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(20..595)
                     /locus_tag="OO161_004186"
     CDS             complement(20..595)
                     /locus_tag="OO161_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5465007.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPGELASALKQQLRIAIKR"
     gene            complement(610..963)
                     /locus_tag="OO161_004187"
     CDS             complement(610..963)
                     /locus_tag="OO161_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5465008.1"
                     /translation="MRDFENSFDAALARVDRTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(956..1324)
                     /locus_tag="OO161_004188"
     CDS             complement(956..1324)
                     /locus_tag="OO161_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465009.1"
                     /translation="MATKEENQKRLRELAGLLGREADMSGSAADIAQRVAEWEEEICA
                     SENEIADVDDTVHEQACRHTGEDDVGILERIRLLKCFYLCGVDDETGEPVEHVDAGRV
                     ILMPPSVAKDMVRSGMAVYA"
     gene            complement(1376..>2215)
                     /locus_tag="OO161_004189"
     CDS             complement(1376..>2215)
                     /locus_tag="OO161_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5465010.1"
                     /translation="RHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYR
                     RLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKNNIIQG
                     SGKEWSKQDRDTFDPTYDIDLFCDQASGLVNIAIMDGTVWRLLNGFKLFREKMDTRRG
                     SNSQLETAVKDLGAVVSFKGYYGDLVIAVAKTSYVAEDGTEKRYLPEGTLVLGNTAAE
                     GIRCYGAIKDAQALSEGVVASSRYPKHWLTVGDPSCEFTMTQSAPLMVLPDPDEFVVV
                     QVK"
BASE COUNT          496 a          686 c          493 g          540 t
ORIGIN      
        1 gcacggatat cagtgtgttt catcgtttta tcgcaatcct cagttgttgt ttcagcgcgg
       61 atgccagttc tccgggcagg cgttcacgcc ggatacggtc aacattctca tcaaatgcct
      121 gtttcagtgg ggccgccatc gggattttca ccacctgaat gggaaggcga ttacgctttt
      181 tccttccctt gtcgtcattg ccctcctcat atctggcctg gggaagacgt tgcataacat
      241 gccagcgccc attatttaat cgctggataa atgcccgctg ataacgatgc tgaccggctt
      301 tgagtatgct gttcggacga cgccccagca ttctgatccc cagcttaatc acagggagat
      361 caccgcggtt aacgataatt ctggcattcg gatttctgac cgtcgcccgt ttcagtctgg
      421 accgttcctt taccagtttc cgtctcaccc tggtttcccg ggcaacctgt gacgaagact
      481 gattaatcgc cgttgtggcc acgcggttaa tggtcattgc agaagcagcc ggaatggcgt
      541 ttttacgaac ccggctcaga ttgtcaatcg cctgatcaag cccttttatc gccataattc
      601 accctgcgtt tatcgtcgcc ggttaactgc gggtggttgc ccacggttga gccagagata
      661 acagctaccc ccgtcatccg gagaaacacg atccacccag aatgtctcac cattaatggt
      721 cagcgtgtca ccacgccgca cggcacgaac cgtatccgtc cgcacaaata atgacgggct
      781 gcttccttca atacggaccc cgctaccggc aaaccccagc gactccggat cgtcaaaaac
      841 cccctgaact tcgctgccac actgtgcccc cgaggtgaac tgcgcacaga gccccatcac
      901 ttcaacaatc gttctgtcca ccctggcaag ggcagcatca aaggaattct caaaatcacg
      961 cataaacggc cattccgctc ctgaccatgt cttttgccac tgaggggggc atcagaatta
     1021 ctctgccagc atcaacatgc tcaacaggct cacctgtttc atcgtcaaca ccgcacaggt
     1081 aaaaacactt cagaagcctg atacgttcca gaataccaac gtcatcctca ccggtgtgcc
     1141 tgcatgcctg ctcatgaacg gtatcatcaa catctgcgat ttcattttcc gaggcgcaaa
     1201 tctcctcttc ccactctgcc acacgctgtg cgatatccgc agcactcccc gacatatccg
     1261 cctcgcgccc cagcagacca gccagttcac gaagacgttt ctgattttct tcttttgttg
     1321 ccatatccag ctccctgtga aaaaaacacg ggggcatttc gcccccgctc acggattatt
     1381 tcacctgtac caccacaaac tcatccggat ccggcagcac catcagcggc gcggactgcg
     1441 tcatggtgaa ttcacaggac ggatcgccca cggtcagcca gtgtttcggg taacgggaag
     1501 aagccaccac tccttcagac aacgcctgtg catccttaat ggctccatag caacgaatcc
     1561 cctctgctgc cgtattcccc agcaccagcg tgccctccgg cagataacgt ttttcggtac
     1621 cgtcctctgc cacataagac gttttcgcta ccgcaatgac cagatcgccg taatacccct
     1681 tgaaggatac cactgcgccc agatctttca ctgccgtttc gagttgtgaa tttgaaccgc
     1741 gacgggtatc cattttttcg cggaaaagct taaagccatt cagcagacgc cagacggtac
     1801 cgtccataat ggcaatattc acaagaccac tggcctgatc acagaaaagg tcgatatcat
     1861 atgtcgggtc gaacgtgtca cgatcctgtt ttgaccactc cttaccgcta ccttgtatga
     1921 tgttattctt cgtcgatcgg ccaaaatcga cttcaatttt ctcgaactgg tctccttcca
     1981 tcgtatattt gccatacagc acggcattca ccgcctgcat ttcttccacc tgcacaatag
     2041 cgtgctcttc ctgtttgagg ttatcagtga tgatacgcag acgacggtag gccgggtcgt
     2101 tcagctgagc cggatcttca ccgggaaggc gctcaaccgc ctgctggtaa ttaaattcgt
     2161 gtttgggctt gacgtagccc ggacgcaaca cgcgggtttc accaccacga tggcg
//
LOCUS       DAJTNA010000331         2198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTNA010000331 DAJTNA010000000
VERSION     DAJTNA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.338"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..1643)
                     /gene="fimD"
                     /locus_tag="OO161_004190"
     CDS             complement(<1..1643)
                     /gene="fimD"
                     /locus_tag="OO161_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5465011.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNAASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWCLRDNTSWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDICDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNTQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGTLGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSG"
     gene            complement(1710..>2198)
                     /gene="fimC"
                     /locus_tag="OO161_004191"
     CDS             complement(1710..>2198)
                     /gene="fimC"
                     /locus_tag="OO161_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5465012.1"
                     /translation="KDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDRESLFWMNV
                     KAIPSMDKSKLTENTLQLAIISRIKLYYSPAKLALPPDQAAEKLRFRRSANSLTLINP
                     TPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALTPKMTGV
                     ME"
BASE COUNT          579 a          526 c          490 g          603 t
ORIGIN      
        1 ctaccactca aatacagtgt tgatgtgcgc ccgagttgct gagtaacggt gagctgtaat
       61 ttcccgcgtt tgttataagc gaggttgtaa tagtcggtga atttcggctt aacctgaata
      121 actccatcct gtgtttcgat gttgtagcca ttcattcgac tgtatgttgt atcagcgaaa
      181 ttaaaatatc cgctggtcga ataacggtaa cccactaact gaatattcgt gcctgactca
      241 ttgagcgatt tgttatagag aaaacgcacc gattgtccgt catgctgact gtcatcggga
      301 agtgtggaat tagcctgcgt catatccaca gacagagcgc ccagtgtccc catgtttttc
      361 ccgataccga aattaaaagc acgataacga tccgccagtt gcgttccacc atatattgtc
      421 cagccagccg gaaggccgtg gagtaatgta ctttggaaaa agcggggttt ttcctgctgc
      481 gtatttccac tacggtattc tcctgccgta atggaataac gagtatgccc ttcacgttgc
      541 aaaagcggga ctgacgaata gggtacggta aaaatctgcg tgctgccgtc agcctctttg
      601 atcgttacct gcaagtcacc actattacct gcggcataga tatcgttgat ggtaaaaggc
      661 cccggtggca ccgtactatt ataaatgtca tacccatttt gtttaatagt gacctgtgca
      721 gtaccacgag caataccgtg gatcaccggg gcaaatcctc tttggctatc gggtaacata
      781 ttgtcatctg aggccaattg tgcgccgcga aagttaatac catcgcaaat atcgccctgg
      841 gtataaccat cacccagcgt cagccgggaa cgtaacggta ttatgtctcg ctcaagccag
      901 gtattgatat gctgccattt atttttgcta cctgatgatc tgtcgctact gttataactc
      961 cagctggtat tgtcgcgtaa acaccacgca ccaatattta acccactctg taggtttaaa
     1021 tatgcataat ggctgttacc cccaatccga ttctgtacac tatttccgct gaaattataa
     1081 ttgagcaatc cggcattaat accgggatcc cataactcag gaggaatata accacgcgcg
     1141 cgattactca taaatgcctg agggatcgtc aggttcagtc gctgctgacc aacatccaga
     1201 tgcgcagtag cgtcatggat cattgaggtt aatggcacgc aggcatcatc tgccagcaga
     1261 ttcataccgg agacagaagc cgcattcagc cccatactgg caagttgcgc gcgtgtcagg
     1321 cagggaacaa tcccttgttc actgtcgccc gtattaaatg tgacatcacg cgttgccata
     1381 taaccattat tcaaatagat atcgacgcga tacgtccctg gcggtaattc ttgcccattt
     1441 tcaaaacgcg ataaatcggc cacagcctgg ggatcatccg ctaaaaagcg cggattaaaa
     1501 tagagttcgg cagatgacaa aggtgcctgt gcggcaaaag cacaggcgac aacgagccgg
     1561 acaaaaaaac cagccaaacg atgcttacga atatgcaagc attgtgtgtt tcgctggtaa
     1621 agtcttaaat tcagatatga cattattcct ccggtttaaa attacccggc agtaaattct
     1681 ttttattcag gcgagaaatt ccccctgcgt tattccatta cgcccgtcat tttgggggta
     1741 agtgcgccat aatcatttat tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat
     1801 ttaaccgtgc tttcgcccat tggaggcacc aatgcatttt caagaacccg ggttccggca
     1861 ttcaactctg ttaccgtcag ataatagggt gtcgggttaa tcagcgtcag agaattcgcg
     1921 ctacgacgaa atcttaattt ttctgcggcc tgatcgggtg gcaacgctaa tttagccggg
     1981 ctatagtaca gtttaatgcg gctgataatt gcgagctgta gcgtattctc agtcaatttt
     2041 gatttatcca ttgacggaat cgctttaaca ttcatccaga ataaactttc ccggtcctgt
     2101 ggcaattggt tatttgttgc atcaagaata cgcaaggtat tctctttttt tcccttcatc
     2161 gcaaacagag gaggcgtcac gataaaacga ccatcctt
//
LOCUS       DAJTNA010000332         2189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTNA010000332 DAJTNA010000000
VERSION     DAJTNA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.339"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..840
                     /locus_tag="OO161_004192"
     CDS             <1..840
                     /locus_tag="OO161_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5465013.1"
                     /translation="RHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYR
                     RLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGNNIELA
                     DGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRG
                     SNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAAE
                     GIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVV
                     QVK"
     gene            892..1275
                     /locus_tag="OO161_004193"
     CDS             892..1275
                     /locus_tag="OO161_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5465014.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLNNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVMVPSPLATAMIQHGTAEHA"
     gene            1268..1621
                     /locus_tag="OO161_004194"
     CDS             1268..1621
                     /locus_tag="OO161_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5465015.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQRGSEVQGVFD
                     DPESLGFAGGGGRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRISPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1637..2170
                     /locus_tag="OO161_004195"
     CDS             1637..2170
                     /locus_tag="OO161_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5465016.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     KELAYALKQQLRIAIKR"
BASE COUNT          512 a          514 c          686 g          477 t
ORIGIN      
        1 cgccatcgcg gtggtgaaac ccgcgtgtta cgtccgggct acgtcaagcc caaacacgaa
       61 tttaattacc agcaggcggt tgagcgcctt cctggtgaag atccggctca gctgaacgac
      121 ccggcctacc gtcgtctgcg tatcattacc gataacctca aacaggaaga gcacgccatt
      181 gtccaggtgg aagaaatgca ggcggtgaat gccgtgctgt atggcaaata caccatggaa
      241 ggggatcagt ttgatactgt cgaggtggat ttcgggcgct ctgaaggaaa taacattgag
      301 ctggctgacg gtaaaaaatg gtctgagcag gaccgtgata cgtttgatcc gacgcatgat
      361 attgacctct actgcgatca ggccagcggc cttgtgaata tcgccattat ggacggtacg
      421 gtctggcgtc tgctgaatgg ctttaagctg ttccgcgaaa aactggatac ccgtcgcggc
      481 tcaaattcac aactcgaaac ggcagtgaaa gacctggggg cggtggtgtc cttcaagggg
      541 tattacggcg atctggccat tgtggtggcg aaaacgtctt atgtggcaga ggacggtacc
      601 gaaaaacgtt atctgccgga gggcacactg gtcctgggga atacggcagc agagggcatt
      661 cgttgctatg gtgccattca ggatgcgcag gcgttgtccg aaggtgtggt ggcctcttcc
      721 cgttatccga aacactggct gactgtgggc gatccggccc gtgaattcac catgacgcag
      781 tccgcaccgc tgatggtgct gccggatccg gatgagtttg tggtggtaca ggtgaaataa
      841 tccgtgagcg ggggcgaaat gcccccgtgt cttttttcac aggaggctga gatggcaaca
      901 aaagaagaaa atctgaatcg tcttcgtcaa ctggctggcc tgctggggcg cgaggcggat
      961 atgtcgggga gtgctgcgga tattgctcaa cgtgtgtctg agtgggaaga ggagcttgct
     1021 gtttccccgg agggcattat gcactctgat gagagcgggg ctgatcaaaa tcacacagac
     1081 gatggtgagc agttgaacaa cacggatgct acggatgatg ttaaagcggt ccgtgtgcgg
     1141 aaatgcctgc atgtgatggg gtattgcccg gagacaggcc gtcccgttga actgacgtac
     1201 cggggcatgc gtgttatggt gccatcacca ctggcgacag ccatgataca gcacggaacg
     1261 gctgagcatg cgtgattttc agaatgcctt tgatgctgcc cttgccgggg tggacagcac
     1321 gattgttgaa gtgatggggc tctgtgcgca gttcacctcg ggagcacagc gtggaagcga
     1381 agttcagggg gtttttgacg atccggagtc gctgggtttt gccggtggcg ggggccgtat
     1441 tgaaggaagc agcccgtcat tatttgtgcg gacggatacg gtgcgtgccg tgcggcgtgg
     1501 tgacacgctg accattaacg gcgagatgtt ctgggtggat cgtatttctc cggatgacgg
     1561 gggcagctgt tatctctggc tcaaccgtgg tcaaccaccc gcagttaacc ggcgacgata
     1621 aacgcagggt gaaattatgg cgataaaagg gcttgatcag gcgattgaca atctgagccg
     1681 ggttcgtaaa aacgccattc cggcggcttc agcaatggcc attaaccgcg tggccacaac
     1741 ggcgattaat cagtcttcat cacaggttgc ccgggagaca aaggttcgcc ggaaactggt
     1801 taaggaacgg tccagactga aacgggcgac ggtcagaaat ccgaatgcca gaattatcgt
     1861 taaccgcggt gatctccctg tgattaagct ggggatcagg atgctggggc gtcgcccgaa
     1921 cagcatactt aaagccggtc agcatcggta tcagcgggca tttattcagc gattaaaaaa
     1981 tggtcgctgg catgtcatgc agcgtgtggc cgggaaaaac cgttacccca ttgatgtggt
     2041 gaaaatcccg atggcggccc cactgaaaca ggcatttgat gagaatgttg accgtatccg
     2101 gcgtgaacgc ctgcctaaag aactggcata cgcgctgaaa caacaactga ggattgcaat
     2161 aaaacgatga aacacactga cattcgtgc
//
LOCUS       DAJTNA010000333         2170 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTNA010000333 DAJTNA010000000
VERSION     DAJTNA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.340"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..112
                     /locus_tag="OO161_004196"
     CDS             <1..112
                     /locus_tag="OO161_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR5465017.1"
                     /translation="WAAIGVLGSLLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            117..461
                     /locus_tag="OO161_004197"
     CDS             117..461
                     /locus_tag="OO161_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5465018.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKSPPKW"
     gene            complement(427..699)
                     /locus_tag="OO161_004198"
     CDS             complement(427..699)
                     /locus_tag="OO161_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465019.1"
                     /translation="MDNNTISLQELLDSISRLREDVNTLTVAFSYLAFSIPREQMQST
                     LASIQFESCNPKWSQEQQDSFRRLAVLLDEKYAGKITISADSSENP"
     gene            805..1338
                     /locus_tag="OO161_004199"
     CDS             805..1338
                     /locus_tag="OO161_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5465020.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLSEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1498..1635
                     /locus_tag="OO161_004200"
     CDS             1498..1635
                     /locus_tag="OO161_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465021.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            1637..2104
                     /locus_tag="OO161_004201"
     CDS             1637..2104
                     /locus_tag="OO161_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5465022.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKNVREL
                     KLANAAITDMQMRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGP
                     VREATGTARVDNATGPRLADTVTRDYFTLRERLMTMQKQLEGAQEYIRTQCLK"
BASE COUNT          561 a          477 c          614 g          518 t
ORIGIN      
        1 gtgggcggca ataggcgtac tggggagtct gctgtttggg ctgctgacat atctgactaa
       61 cctgtatttc aaaatcagag aggaccgtcg taaggctgca cggggagagt aattcaatga
      121 ctcaaaacta tgaactgatt gtgaaaggga tccgcaattt tgagaataaa gttacggtaa
      181 ctttagcgtt acgggacaaa aaacgctttg acggtgaaat ttttgacctg gacatctcgc
      241 tggaccgtgt tgaaggtgcc gcgctggagt tttatgaggc agcagccaga aggagcatca
      301 gacaggtctt cctggatgtt gctgccgggt tatgtgaagg ggatgagcag tcgccggaaa
      361 agcgccccgt aattttagag gcgcaggatg tgttgataac ctacagagga aaactaccgg
      421 gaataattac gggttctctg aagagtccgc cgaaatggta attttaccag catatttttc
      481 atccagtaat acagcaagcc gcctgaaaga gtcttgttgt tcctgagacc atttgggatt
      541 gcatgattca aactggattg atgccagcgt tgattgcatc tgttcccttg gaattgagaa
      601 tgccagatat gagaaggcga cggtaagggt attcacgtct tcccgaagcc tggaaatgct
      661 gtcgagcaac tcctgtagag aaatggtgtt attgtccata aataatcctc atgattgtat
      721 tgacctgtta gcagcctgag gcaacaggct ggaactgata aacatatcca gggctcagaa
      781 accgataaat cctgataaat atccatgaac gcaaaaatca gatacggcct gtcggctgcc
      841 gttctggcgc tgattgccgc tggtgcgcct gcgcctgaca ttctcgacca gtttctggat
      901 gaaaaggaag gtaaccacac cacggcatac cgtgatggcg cgggtatctg gaccatctgc
      961 cgcggtgcca tcctggtgga tggcaaacct gtcgttccgg gcatgaagtt gtcgaaggaa
     1021 aaatgcgacc gggttaacgc cattgagcgt gataaggcgc tggcatgggt ggagaaaaac
     1081 atcagagtgc cattgagcga accccagaaa gcggggatcg cgtcattctg tccgtacaac
     1141 attggtcccg gtaagtgttt cccgtcgacg ttttataaac gaattaatgc aggtgatcgc
     1201 aggggagcgt gtgaggcgat tcgctggtgg attaaggacg gtggcagaga ctgccgtatt
     1261 cgttcaaaca actgctacgg tcaggtatcc cgtcgtgacc aggagagcgc gctggcgtgc
     1321 tggggtatcg acagataagc agaatatttt gctgaaaaat gcggtttgct tacacggacg
     1381 gataacacga aatcctgcga actgacaaaa actaagtgaa taaaagtaaa aaacccgttt
     1441 gttggctgca agcggggttt tgtgtttcct gactctggaa aagtcaaagg agaaagtgtg
     1501 tttgatttta gcaaactgat tcgggagatt cgagtgatgg ctgaaaaatt atccacctgg
     1561 aagttcattc ttatctggct ggtgtttgtg attatggcct ccggttattt catcggtcag
     1621 atacgctggt ggtgaaatga accgcgtact gtgcgtggtc atcattgccc tgctggtggc
     1681 ctgtggtgcg cttagtctgg ggctgaatca ttaccgtgat aacgccatta cctacaaagc
     1741 ccagcgcgac aaaaatgtca gagaactgaa gctggcgaac gcggcaatta ctgacatgca
     1801 gatgcgtcag cgtgatgttg ctgcgctcga tgcaaaatac tcgagggaat tagccgatgc
     1861 gagagctgaa aatgaaactc ttcgcgctga cgttgccgct ggtcgtaagc gcctgcgtat
     1921 caacgccacc tgtccaggtc ccgtgcgtga agccaccggc accgcccgcg tggataatgc
     1981 aaccggcccc cgactggcag acaccgttac acgggattat ttcaccctca gagagcggct
     2041 gatgacgatg cagaagcaac tggaaggggc gcaggaatat atccgtactc agtgcctgaa
     2101 ataagttttg ttgatgcgcc gtatcgtcgc tgtattccct cattaacaga gaccgcagcc
     2161 cgacagggag
//
LOCUS       DAJTNA010000334         2166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTNA010000334 DAJTNA010000000
VERSION     DAJTNA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.341"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(78..1139)
                     /locus_tag="OO161_004202"
     CDS             complement(78..1139)
                     /locus_tag="OO161_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5465023.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRVRQYDFP"
     gene            complement(1139..1504)
                     /locus_tag="OO161_004203"
     CDS             complement(1139..1504)
                     /locus_tag="OO161_004203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465024.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            1589..1918
                     /locus_tag="OO161_004204"
                     /pseudo
     CDS             1589..1918
                     /locus_tag="OO161_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            1948..2031
                     /locus_tag="OO161_004205"
                     /pseudo
     CDS             1948..2031
                     /locus_tag="OO161_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            2031..2126
                     /locus_tag="OO161_004206"
                     /pseudo
     CDS             2031..2126
                     /locus_tag="OO161_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          536 a          600 c          513 g          517 t
ORIGIN      
        1 gtagcaacag ctcacccatg cggctctgac aacgatgtta acccgcgtta aatatcccac
       61 gttggccagc atctgtctca cggaaagtca tactgcctta ctctccaggt gatttgcatc
      121 gccgtttttc tcactgtctc cgttatgatg tacaccactt cttccagtaa ccgccgtttc
      181 gccggactca gaaaaccgta gtacctcacc atacggaacc ccttatccgc cacatgccag
      241 gagaaccttt ccatgaactc atctccactc atcaacaggt attcttcccg ttttgttcgg
      301 tgactgttgt aacgcagtcc gatttcatcc tgaccggcat aatgctccag acgactcacc
      361 ggcaccggtg gctttttcag gtaagagcca aagtacaccg ccacatgggt ggcattatcc
      421 atcacccggg atacgttgac attccagcca cggcggtaat gcgtgtctag gaagcgattc
      481 cattcccgtt tactgcttcc ttctgctgcc agcgcatccg gcatcaccag gtcagggtat
      541 ttccgtgaca gtaaccgtgt tatccggtag cgccacatgc tcatcacctt acgggcgtaa
      601 aaatgaagat ttttccaggt gtggcccgac gtcacaccac cggcagttgt cgataaatgg
      661 atatgcggat gccactgctg gtcacgcccc catgtgtgga tcaccgtgaa tatcctcggc
      721 tccacatctg cctgatggca gatttccagt atcacatccg ctgcaatgcg gctcatctct
      781 gccagcaacc accggttgtg gaacaccagg gaccagtact ggcagggtaa cgtgaacaca
      841 atatgctgcc atgggcagtc ggggaccagg ctcagcagat actgtatcca ctgtgcgcca
      901 gccttcaccc cgcagtgcgg gcaggagcgg cttttacacc ggaagcagac cttttttgta
      961 tggcaacagt ccggtgatga acagcaccac tgtgtatacc ccatcagtgt ggtcccgcac
     1021 gccatgattt tggtcaccga ctcaatcacc accggacgta ctgccccttc cggctgcttc
     1081 tccagccagt taagccagcg gtttccctgc tgaaagatat cggcaaaacg gggaagcatc
     1141 agaagggcgg ggcgactccg tccggccagt gaaccgtgcc acactccggg cagtacatac
     1201 cgccggcgct gataccggaa agaatggtcg caaattcccg ctccgtgcag cgggcgattt
     1261 ccggataccc ttcgtcatca acacgtacaa accagaagac cagctttttg tttcccgcat
     1321 ccacaaagaa cggaatattc aggtctgcgc agcattcaac ggcatcgtca aaactatcaa
     1381 agcgcagaac ttctgcgtct tcttcgtcaa aaaaatcatc ttcgtgaagc ttcacgacat
     1441 agcggggaag tttgcttctt tgagaggcgg gtttacgttt acggggttta gctgaacggg
     1501 ccatataacc acctgaaaga caatgacatt gcctgttttt ataacggtaa ttgcagacca
     1561 tgacaagccg cagccgtcag gctgcctact cgagcttcag ctcaataaag gcgagtatcg
     1621 acataaatta ccccgatgga tcttttttgc aaatcagggt gaattcacga ccggtgatta
     1681 cgaagaaatc atgaataagg ccagcgccct gagtctggta tcgaatgcaa tcctgtactg
     1741 gaacacatca agaataagca atattgttga agggttacgt gaaaatggtg aggttattga
     1801 tgatgacgta ttagcccata tatcattact gccctataaa cacgtattac ctaatggtac
     1861 ttattttgtt gatggagaac cttacggtac tgagcctacc gaaagagcaa aaccgtaagt
     1921 ttctgtttaa atgctcccgc tggtccatcc ggacagatta tcgaccatca ggctgtacag
     1981 gcctgggcgg acagcctcag tactgacaat ccgttaccgg tgccacgctg atggaacaga
     2041 agtggaccag taaggcgctt tctgatttgg cgcggttata tgattttctg gtgctggcca
     2101 gtaaacctgc tgccgccaga atggtacacg tataggaaat tgaaaaacca gacgataccg
     2161 ctgtcc
//
LOCUS       DAJTNA010000335         2149 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTNA010000335 DAJTNA010000000
VERSION     DAJTNA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.342"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..165
                     /locus_tag="OO161_004207"
     CDS             <1..165
                     /locus_tag="OO161_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HCR5465025.1"
                     /translation="ASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNISLDEFA
                     KALKSVLARI"
     gene            complement(427..>2149)
                     /locus_tag="OO161_004208"
     CDS             complement(427..>2149)
                     /locus_tag="OO161_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5465026.1"
                     /translation="HVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTM
                     TPERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSAN
                     GTPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQ
                     TQTIVKVTGNSSTAHVASFIAAPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALK
                     SGSATLTSLTAVTDQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADTSQ
                     SVLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNVPYVQVSAIDYS
                     KNFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDL
                     PTTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTYKNVGSNWE
                     RITATPKSGGPSYVYEIRVKSWWVNAGDAFMIYSLAENFCSSNSYTLPRADHLNHSRS
                     RGIGSLYSEWGDMGHYTTEAGFQSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAY
                     ATCYKNL"
BASE COUNT          542 a          501 c          502 g          604 t
ORIGIN      
        1 gcaagtgcct ataatacccc cgttgttgca atttatgctg attacaaaac gcgatggccc
       61 gcaatggccg atgtttcgga gtcagtcgtc gttgggcaaa aaattgacaa tataagtctg
      121 gatgaattcg caaaggcatt aaaaagtgtt ttggcgagaa tatgagacag tacttatgca
      181 tagtaatgcc gatctgttat gaacggtggt gtcatcaata ctctgaggct ggtcaatcat
      241 cacagaaaac cagcctttgg atcaaagctg tactcaccgg atacgatcat agtcacaccg
      301 aacgcgaatc tggcggtatc gcatttttat ttattcattt tcggtgagag aagagcaagc
      361 tctatagagg gaatgtaaaa aagagtgccc cgaatggagc actatttaag aaattataac
      421 ggaaaatcag aggtttttat aacatgtcgc ataagcaaac ccaagctttt caaatacgct
      481 ttgatcacct gttgccaggg aaactacgta ttgttcgttt gagtttgcgg gactagatga
      541 ccaatacata tttgattgaa aaccagcctc agtcgtgtaa tgccccatat ctccccattc
      601 actgtacagt gacccgatgc ctcgggaacg actatggttt aaatgatctg cacggggaag
      661 tgtgtagcta ttgctactgc aaaaattttc agcaaggctg tatatcatga aagcatcgcc
      721 ggcgttcacc caccaactct tcacacggat ttcgtataca tagctagggc ctcctgattt
      781 tggcgtcgcc gtaatccttt cccaattgct gccgacattt ttatatgtca ctttaccggt
      841 agcatcaaca tcgacccagg aggcagagct tgaaaactca taatcagccg ccgtttttcc
      901 aggggcaaag ttgtcattat tcaactgata atacgccccg gtgaatccct gcgaagggaa
      961 tgtagttgtc ggtaggtcag taccattgac tgacactgta ccgctcatta ttttgtcttc
     1021 tgcgcgagtg aattgtattg tggtactcag accagcttga tgaacaccat tcaatacagg
     1081 gatcagcgtt gcgataccct ctccgccgcc tgtaacagtg gctttgtact cgcctgagaa
     1141 atttttactg tagtcaattg cactaacttg cacatagggc acgttggtac ctgactgcac
     1201 aaattccagc ccttcagaaa ctttgatcgg attgcctgat atatcgtgca gaacaagatg
     1261 tagctcagca ctatcggtaa agtctccttt caatgatgac cgattgttct taaggacgga
     1321 ctgcgaggtg tctgccgggc cagccaccag cgttatatct actgtttgca ttccaccagc
     1381 ggtcgtgact gcgcttaccg tgacgctacc tgtcatcgct cctttcacgc ttgttgtcgc
     1441 gattccgttt tgatcggtca ccgctgttaa tgacgttaat gtggcagagc cgctttttaa
     1501 ggcgaagtac acagttaaac cttcgatcag gttaccactg ccatcctcaa ccgttgcctt
     1561 taaggtactt aaatcactgt tggtggcggc gatagtcgat ggagcagcga taaagctagc
     1621 aacatgggcg gtgcttgagt tgccagtgac tttcacgatt gtctgtgtct gtattgttac
     1681 accgttatgg aacgatgcag tcaccgtata tgtaccgact ttattgctgg tcagaacgac
     1741 tggagcctga cctgaagagt tggttctcac ttttccgcca cttaacgtcg caccttctgg
     1801 cgttacgcta aagttgatga cctgaccctc cactggagtg ccatttgcag aagttagcgt
     1861 tgccgtcaga gttgcacctg ttggggaatt gacaccgata agcgggctgg acgccgagag
     1921 tgtcagtttt tgatcgatta ccaccagatc cttctcataa gaggatccat tcgccaggga
     1981 tgctttcacc atatacgaac cgtttctttc gggcgtcata gtgacctcgg ctataccatg
     2041 cgtatcagta gagacaatat tttggctgat ggtcatgtgc tctggtggct ctgcactgaa
     2101 tgttacagat tcattaagaa tcggattgcc aaactggtcg ttgacgtga
//
LOCUS       DAJTNA010000336         2135 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTNA010000336 DAJTNA010000000
VERSION     DAJTNA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.343"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..740
                     /gene="psuG"
                     /locus_tag="OO161_004209"
     CDS             <1..740
                     /gene="psuG"
                     /locus_tag="OO161_004209"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5465027.1"
                     /translation="IGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNGAT
                     TVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSIL
                     DLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARAMAVKWQSGL
                     NGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTLFLLARVAELTGGDS
                     LKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            834..2084
                     /locus_tag="OO161_004210"
     CDS             834..2084
                     /locus_tag="OO161_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5465028.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
BASE COUNT          468 a          483 c          602 g          582 t
ORIGIN      
        1 ttattggcgg cgtgatgaaa gtgggtttaa gcaaagaaga aattgaatta ctgggtcgtg
       61 aagggcataa cgtgaccaaa gttagtcgtc gcgatttacc ttttgttgtt gccgccggaa
      121 aaaatggcgc aaccactgtg gcttcaacga tgattattgc ggcgcttgcc ggaattaaag
      181 tatttgccac cgggggaatt ggtggtgtgc atcgcggggc ggaacatacc ttcgatattt
      241 ctgccgattt gcaagaactg gcaaatacta atgtcaccgt tgtttgtgcc ggggcgaaat
      301 ctattctcga tttaggatta accactgagt atttagaaac cttcggtgtg ccgttaattg
      361 gctatcagac taaagcgctg cctgcgtttt tctgccgtac cagctcgttt gacgtcagta
      421 ttcgtctcga cagcgccagt gaaattgccc gtgcaatggc ggtgaaatgg caaagcgggc
      481 tgaacggtgg cctcgtggta gcgaacccga tcccggaaca gtttgcgatg ccggaggaat
      541 ctatcaatgc agccatagat caagccgtcg ccgaagccga agagcagggc gttattggta
      601 aagaaagtac actgttcctg ctggcgcgcg ttgctgagct gaccggcggt gacagcctga
      661 aatccaatat ccagctggtg ttcaacaacg ccattctggc gagtgaaatt gccaaagaat
      721 accagcgtct cgcgggttaa tttctgacgg gcagaaagcc gtcgcccgta ctcatcaagc
      781 ataccaaaac ctggcgctga cgccgggttt actggcatga agggaaaatc attatggata
      841 taatgagaag tgttgtgggg atggtggtgt tactggcaat agcatttctg ttgtcagtga
      901 ataaaaagag catcagtttg cgcacggttg gagccgcact gctgctgcaa atcgctattg
      961 gtggcatcat gctctacttc ccaccgggaa agtgggcagt agaacaggcg gcattaggcg
     1021 ttcataaagt gatgtcttac agtgatgccg gtagcgcctt catttttggt tcgctggttg
     1081 ggccgaaaat ggatgtcctg tttgacggtg cgggttttat cttcgccttt cgcgtacttc
     1141 cggcgattat tttcgttact gcgctcatca gtctgctgta ctacattggc gtgatggggc
     1201 tgctgattcg catccttggc agcattttcc agaaagctct taacatcagc aaaatcgaat
     1261 cttttgtcgc agtcacaact attttcctcg ggcaaaatga gatcccggcg atcgttaaac
     1321 cgtttatcga tcgcatgaat cgcaacgagt tgtttaccgc aatttgtagc gggatggcgt
     1381 ccattgctgg ttcgatgatg attggttatg ccggaatggg cgtaccaatt gactacctgt
     1441 tagcggcatc gctgatggcg atcccaggtg gtattttgtt tgcacgtatt cttagcccgg
     1501 ccaccgagcc ttcgcaggtc acatttgaaa atctgtcgtt cagcgaaacg ccgccaaaaa
     1561 gctttatcga agcggcggcg agcggtgcga tgaccgggct gaaaatcgcc gctggtgtag
     1621 cgacggtggt aatggcgttt gtcgcaatta ttgcgctgat caacggcatt atcggcggaa
     1681 ttggtggctg gtttggtttc gccaatgcct ctctggaaag tatttttggc tatgtgctgg
     1741 caccactggc gtggatcatg ggtgtggact ggagtgatgc caatcttgcg ggtagcctga
     1801 ttgggcagaa actggcgatt aacgaattcg tcgcttacct gagtttctcc ccatacctgc
     1861 aaacgggcgg cacgctggaa gtgaaaacca ttgcgattat ctcctttgcg ctttgtggtt
     1921 ttgctaactt tggttctatc ggtgttgtcg ttggcgcatt ttcggctatt tcgccaaaac
     1981 gcgcgccgga aatcgcccag cttggtttac gggcgctggc agcagcaacg ctttccaacc
     2041 tgatgagtgc gactattgcc gggttcttta ttggtttagc ttgagtttca ttgccggatg
     2101 tacgcgctta ttaagcgttg catccggcaa tcgcg
//
LOCUS       DAJTNA010000337         2106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTNA010000337 DAJTNA010000000
VERSION     DAJTNA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.344"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..643)
                     /locus_tag="OO161_004211"
                     /pseudo
     CDS             complement(1..643)
                     /locus_tag="OO161_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            complement(645..923)
                     /locus_tag="OO161_004212"
     CDS             complement(645..923)
                     /locus_tag="OO161_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465029.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            864..1049
                     /locus_tag="OO161_004213"
     CDS             864..1049
                     /locus_tag="OO161_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465030.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(1091..1246)
                     /gene="hokD"
                     /locus_tag="OO161_004214"
     CDS             complement(1091..1246)
                     /gene="hokD"
                     /locus_tag="OO161_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5465031.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          517 a          543 c          498 g          548 t
ORIGIN      
        1 gtatgctggt agcggtctgc tctcccggca cgatgtcgct ttcacggtac attgagcgga
       61 ttttttccgc acgcaacccc agcgaacgac gtaataccgc ttccggtagc gcgtccgcca
      121 cctgattgcg gaccgcctac caggataatt cagccagaga taattcacgc tcctgcgtac
      181 cgcttattgc gtgaccgatg acgtcaatca tccatgctga caggttttga tgagcaagtt
      241 gctcgagtga ttcggatgtc tggtcacgca actggttgtc gcagtgccag cacaacacca
      301 ttgcgccggt accataacgg tgaatgacgg tttcgctgtg atgataatcg ccgtgtggcc
      361 actggcagga tttaatatgg cgcaacagcc agtcagacaa tgcaccagca ccaccagcag
      421 cacgaatcac ccgtgcgtta ctgaaaaacg gcagcaatgt tttgtcttcc actagcggct
      481 ggcgaacggc aggaacgacc ccggacggca gattacgcat gcttttcggt tccggctcca
      541 ccagtacccg ggtattgtgg aataccggca tggattcacg gcctggctta aggaccacca
      601 gcccgagttc cgtaaccaga acaggtcgaa gtaatacccg cacattacct ccagatccgt
      661 tgctggaatg tgcaggacgg acgcggtggc cgttcggaat aagggagcct gacgaagatt
      721 atccagtgac gataatcgag actgagggct ttcttaatct cgtatccgtg tctgcggtag
      781 cgctgaatca gccattcagc ctgttcttcg gtgcaggggt cgtgctgata ccagtcagat
      841 ttgaatgcat gagaacgccg cccgtgcctg ctggcagggg cggcagagtt atccgaattg
      901 taaaatttgg tatcgtgcgc catctgtttt ctctgctggc gcagcaggtg ccagttgttc
      961 aggctgacgg atggattgta aaccagaacg accagaaaaa acaaaacccg ccgaagcggg
     1021 ttaagtgcgg gtgcgttgag gatgcctgac acatcagcgg tggcgaggga tttctccccc
     1081 gccgggtctc ttactcctca ggttcgtaag ctgtgaagac agcgacctcc gtctggccgg
     1141 ttcggattcg tacctcgcag aggtctttcc tcgttaccag tgccgtcact atgacggtta
     1201 aacagatgac gataagggcg attaacatcg ccttttgctg cttcatagcc tgcttctcct
     1261 tgcctttcgg cacgtaagag gctaacctac atgtgttcag catggattga gcctcagatt
     1321 aatgttaagc gtcttgcagg acgcgtaatg ttaactgggg cttttctcta tctgcctttt
     1381 ggtgttcatg cctgagacag atagcctcaa gcacccgcag tcattctact taactaagat
     1441 ttccctgcaa accgtttttg tccggcacag taaatatcca actaaaccaa tagcgttcgc
     1501 tgtatttacc gccagtattc aatgcacatg accgccatga acacccctaa aaaaagggca
     1561 tttatatgtc caaacattaa tatcaaaaca tcaatttttt ccatatacct tgctgtgaag
     1621 atgatgggca tacatgatgc gaacaaccag aacgcaacaa acaaaaactg caatgcgttt
     1681 ttcattattc cccctacaat caatgtgcaa taacatttaa acacacctca atttggccgg
     1741 acatataaat atctaaacca gaaaaaatca cttacatagc gttacaaact ctttagtcta
     1801 aatactcatc gtaaaacatt ccccatactt atcagtccgt tccgcgccag gtagcttatt
     1861 gccttatctg gcaacctata atcaggtttc cgctttttca gttggctggt cgtttaaccg
     1921 acatagttaa cccattaatc tggttgccgg atgctggtgg attttcgcgt tttagttgtt
     1981 cataaaagtg cacagcttta accagttctt ctgatgtaac cgggactggt ggggcagtga
     2041 ataaggcctg aatttcatag ttcggcctgt cgttacaatc ctcttttttc ggtacatatt
     2101 tccagt
//
LOCUS       DAJTNA010000338         2103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTNA010000338 DAJTNA010000000
VERSION     DAJTNA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.345"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..833
                     /gene="copD"
                     /locus_tag="OO161_004215"
     CDS             <1..833
                     /gene="copD"
                     /locus_tag="OO161_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCR5465032.1"
                     /translation="TSLMLVFGFAMYGAWLAPLTIRRLLAKRFLRLQQHAAVWSLISA
                     TAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLALVTLIVALMQPRNMP
                     RLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAAWFGGLLPVLWCMQL
                     IKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLSALLITGFPPTLTTYWGQLLLLKAI
                     LVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAVVLVIISLLATLEPF
                     "
     gene            846..1187
                     /locus_tag="OO161_004216"
     CDS             846..1187
                     /locus_tag="OO161_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5465033.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1580..>2103)
                     /locus_tag="OO161_004217"
     CDS             complement(1580..>2103)
                     /locus_tag="OO161_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5465034.1"
                     /translation="NCIREAADQLPAWFPLAMDLALVTGQRREDITNMRFSDIYDDRL
                     HIRQIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGIRKNSPDGSIHP
                     DGLTKKFVAARKLTGIQFSENPPTFHEVRSLAGRLYKETCGEEFAQRLLGHTSEKTTK
                     MYLDEREKTYLLL"
BASE COUNT          484 a          489 c          512 g          618 t
ORIGIN      
        1 tcacctcgct gatgctggtt tttggcttcg caatgtacgg tgcctggctg gcccccttga
       61 cgattcgtcg cttgctggcg aagcgttttc tgcgtttaca acagcacgct gccgtctgga
      121 gtttgatcag tgccactgca atgctcgccg ttcagggcgg gttgatggga acgggatgga
      181 cagatgtatt ttcgccaaac atttggcagg ctgttttgca aacgcagttt ggtggcgtct
      241 ggttatggca aattgttctc gccctcgtaa cgttgatcgt cgcccttatg caaccgcgga
      301 atatgccacg tttgctgttt atgctcacta ccgcgcaatt tattctgctg gcgggagtgg
      361 ggcatgcgac gctgaatgaa ggggtaacgg cgaaaatcca tcagactaat cacgcgattc
      421 acctgatttg tgcggcggcg tggtttgggg gattactgcc ggtgctctgg tgtatgcaac
      481 tcatcaaagg ccgttggcga caccaggcta ttcaggcgct gatgcgtttt tcatggtgcg
      541 ggcattttgc ggtgatcggc gtactggcaa gcggcgtgct tagtgcattg ttgattaccg
      601 gatttccccc cacgcttacg acttactggg gccaactctt attgctcaaa gccatcctgg
      661 tgatgattat ggtggtcatt gcgctggcta atcgttatgt tcttgtaccg cgcatgaggc
      721 aggatgaaga tcgtgcggca ccctggtttg tgtggatgac taaactcgaa tgggcgatag
      781 gtgcggtagt gctggtgatc atcagcctgc ttgcgactct cgaacctttt tgatggatta
      841 cgaaaatgat gaaaaaaagt attctggcgt ttctgttact caccagttct gcagcggcgc
      901 tggctgcacc gcaggtgatt accgtcagtc gttttgaagt gggtaaagac aaatgggcgt
      961 ttaatcgcga agaggtgatg ctgacttgcc gaccgggtaa tgctttgtat gttattaatc
     1021 cgagtaccct cgtgcagtat cctttaaacg atatcgcaca aaaggaagtt gccagtggga
     1081 agaccaatgc ccaacccatt tcggtgattc agattgatga tcctaacaat cccggcgaaa
     1141 aaatgagtct ggcaccgttt atagaacgag ctgaaaaact ctgttaatta cctaaaatag
     1201 ccttttgatt tccaataaaa aaaccgcctc agttctttca ccagaacggg cggtttttaa
     1261 catttcagct gatgaccacc acgcttttta ttgaccattt tgcacgcaaa ctggaaaacc
     1321 tggcgtcgtc atctattctt aaagggcaag gcaactaagc ctgcattaat gccaactttt
     1381 agcgcacggc tctctcccaa gagccatttc tctggaccga atacaggaat tgtattcggt
     1441 ctttttttaa ttgcatttga aatcaattag ttgcaaatgt ctccccgaaa ttccccgaaa
     1501 tttactcgaa tttctgtatt ccggtctttt ttggttatat cacaaccaaa atacatttaa
     1561 caatccattt acgttaaaat cagagcagta agtacgtttt ttctctctca tcaagataca
     1621 tttttgttgt cttctccgat gtgtggccaa gtagacgctg agcaaattct tctccacatg
     1681 tttctttgta caatcgtcca gccaggcttc tgacttcgtg aaaagttggt gggttttcac
     1741 tgaactggat acctgttaat tttctggctg cgacaaattt ctttgtcagg ccgtccgggt
     1801 gaatgctgcc gtcagggctg ttttttctaa tcccggcact gattagataa tcccccctac
     1861 ttaccatgcg gcactgttca actactgcac caagtcgtaa accagcgaca ggaaggctga
     1921 gtgacagggg gatagcaatc atcattcctg tcttaatttg cctgatgtgg agacgatcat
     1981 cataaatatc actgaaccgc atattcgtta tgtcttcgcg acgttgtcct gttacaaggg
     2041 ctaaatccat agctaatggg aaccatgccg gaagttgatc tgctgcctcc ctgatgcagt
     2101 tgt
//
LOCUS       DAJTNA010000339         2100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTNA010000339 DAJTNA010000000
VERSION     DAJTNA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.346"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..684)
                     /gene="ipaH7.8"
                     /locus_tag="OO161_004218"
     CDS             complement(<1..684)
                     /gene="ipaH7.8"
                     /locus_tag="OO161_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5465035.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYR"
     gene            1012..1191
                     /locus_tag="OO161_004219"
                     /pseudo
     CDS             1012..1191
                     /locus_tag="OO161_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1208..1898)
                     /locus_tag="OO161_004220"
                     /pseudo
     CDS             complement(1208..1898)
                     /locus_tag="OO161_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1821..2072
                     /locus_tag="OO161_004221"
     CDS             1821..2072
                     /locus_tag="OO161_004221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465036.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
BASE COUNT          620 a          389 c          469 g          622 t
ORIGIN      
        1 cctgtaactt gaatctaatt gtgaaaaact ctccggaaga gtggttatct gattaaaatg
       61 aaaataatat tccttttgtc tgatattgtt tctatcagaa aattgaggaa aagtaaccag
      121 attattgcgc tgagcacaga gaatttccag tttatccggt aagtcgggta atgttgtcag
      181 cctgttataa taaacatgta aaaatttcaa tttttcggga agagccggta agttatatag
      241 cctgttattt tcaacaaata atgatgttaa atggtttggc aaaacaggaa ggttttccag
      301 ttgattagaa cgaacactta aagttgttaa tgactcagga agttctggta tcacagaaag
      361 cctgttatta tcagcattaa gttctttgag aaatgcaggc agctttggca atgatgttaa
      421 tttattattt tctacaatca aagcagaaat atgctttggt aattcgggaa gagacgtcaa
      481 atttaggtct gataaattta atactgcttc ttgattctga aagcactggg agagtctgtt
      541 aaaagcaatg cctcgctctt ccccgggaga agagttcttt tcccattcag tcaggattct
      601 cagataatgt tcattactgg ttgagtttga gttaatagag ggggagcaag aaactgatga
      661 gtgtgtatta tttacagaga acataatgct agggcaacct tatatttgtg agaataaata
      721 tttaaaattg tatatttata caaatttcac ttaccatttc cgatattaag ttttaatagt
      781 ttccatttta cacataacag aagttagttg taatttattt attgaggttt tctatggaat
      841 tcttttttat tgtgactgca gtcaataata acactgaatt caacattcca gtgagcaagg
      901 aaatgtaatc cgagtaagta tatttgtttt acatcatatt ttagccaagc tcatttattc
      961 ctattataga atgataaatt aaaattactg taaaaaaacg atacacattt aatacgaggg
     1021 aggccggcag aaaaacggct tgcggtaagg caggagaaat gtgtgccgcg aatgaagtcc
     1081 ttgtatgact ggataaagct ccagaggaaa actttgtcaa accatgctga aatggtaaag
     1141 gcgttcaact atatcctgaa tcactggaat gcactgaatg agttttgccg ggtagtactt
     1201 ccaatcatta gaatatgtgc ttttcaatga aggaaccgat gactttttcg tggatttcca
     1261 cggaacgcga gaagcagatt gttttacgtg ccagccgctt gatgcggggg cggagggtca
     1321 ggttgttacg ttcatacgtt gcgtgaagat tttgccggtc aggtgtttct cctccggcac
     1381 ttctctggcg tagctgcccc agtcatcgct ggtcaccatg ccgatacaga acggagtcaa
     1441 cagggctaac agttcccgac aggtctcatc attccgtggc ccgcaagtgt aagccaacac
     1501 accgccagtt ttagtgttgt acgcatacca gggccagtgt tgttgcgcct tgctgccaac
     1561 gaagcgccac tgctcatcaa gttcacagat aagggcaaca tcagcgtgag cgactggcgc
     1621 agatgttatc cgccggggcg agagcttttt aatgtccgta tgacggtatt gatgccgatt
     1681 ttcagtgttc tggcggtata acagccccct gcgccgttat gtgccatttc aacgatctgc
     1741 tccttaacgc cgagttttct ggcttcataa gagtaagtga gctggaaaac acgtttacat
     1801 gagcgacaac ggaaacgctc atgccgggaa caactacggc catgtcgata aacttcatcg
     1861 gaattacagc gcggacaatg aacagtaacg gtagtcatgg aaaacctcaa ataccagtag
     1921 attaccagaa acttctatcg attgaagaca ttaccttttg ccgcaatagg tggggtaaca
     1981 ttggtgaaaa cgcattacgc cgggtggcag ttggaaaaaa attatctttt ttcggctctg
     2041 atcgtggtgg agaaaatgcg gcaataatct aaatagctgc gcttaatacc gctacacttt
//
LOCUS       DAJTNA010000340         2089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTNA010000340 DAJTNA010000000
VERSION     DAJTNA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.347"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..1458)
                     /locus_tag="OO161_004222"
                     /pseudo
     CDS             complement(1..1458)
                     /locus_tag="OO161_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
     gene            complement(1455..>2089)
                     /locus_tag="OO161_004223"
     CDS             complement(1455..>2089)
                     /locus_tag="OO161_004223"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HCR5465037.1"
                     /translation="VLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRG
                     LCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVVY
                     CDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYCNFTH
                     HYIKVKRSIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT          503 a          560 c          502 g          524 t
ORIGIN      
        1 tcaatgttct tactgatgta tttcgcgatg tagcttgtcg gcgttccttt gcgcgggttt
       61 atcagctcat atttaaagcg tggaccagtg ttattaccca gttcctcgcg gtcttcacgg
      121 atggcaaact tacgcaacaa tgcagtaatg gcgcggcggt cttttttgcg cataaaacac
      181 aacaggtgcc agtgaactgt accgtcatga tgcggctcag ccacccgcac gccataccag
      241 cgcaacccgg ctttgtgcat cgccttacga aatgcagcaa acatgccgac cagataatcg
      301 ctgctttgtc ttaccgtcgc atttgtccag gttgggtttg gtctgccgtt attgagcgtg
      361 gaatggaaac gtgacggaca ggtaatggtg tagaaaacgg cgcagtcacc gcgcatttcc
      421 gcgataagct ccagaccttt aacacaggcc atcatctcat tgcggcggtg cgccgggttg
      481 ctgctgctgg cgtttaccac gtcttccata tccagcgtgt cgccctcttc gttcaccagt
      541 tcatgagaac ggaaaaactc caatgacttg cggcgctgct cacgtttatg catcacggct
      601 tcatagctga catagggaga tgcttttttg ctgaccaggc agacagcacg caactgctct
      661 tcccgccatt cgcaacgcat tttccataat ttccggtacc accagtcggc gcacaacata
      721 cgcgccagcg aacccggaat gagttcatag ggcacgggtt tacggcggtt tcttttccga
      781 cggagttgct caaacgcagg cgggatgaca tccagacgca gggtttccgc tgccaccttt
      841 tcccatgtct tgcggatttc ttctggctta acgtcatcgg tggcatacaa atcaccacaa
      901 gctgcatcaa ggcacatgct catatgcgca gcgacaaggg tagacaggcg tttcacctga
      961 tcctgactca tttcaggcag aatcagcagg ccgtccagcc ctacatggct tgccataaaa
     1021 cgaaaagatg cagatagctg gctgtcgcgt acatgctcca gtcgttccag acatggctta
     1081 atcgtctcac gtaaatagcg ggaataagcc tttggcctgc ccaggctgct gaagtattca
     1141 atacgttgca tcagcggctt gctgatatgg gaaggctggg cgttgacgtc cgccagaatg
     1201 accatatccg gattaaaacg ctgctgctca tgcgccagct ttgcccggct aatgagctta
     1261 tcctgctcca tttcgtgctg gacaggatca cgtgattcat aaaaaaaata acgctcccag
     1321 acctgatcac tcagtgcctc gcggcgcaac ttccgccggg tccagataag ggttaatggc
     1381 ctttttcggg ctgttccatg agaacgctgc ggcagcctcg ttaaagccgc agcagttgtt
     1441 catatcggca tggctcatgc acgtactccg tacacggcag aactatccac gccacgcgaa
     1501 taatcaaatc ccacccagca gcgcggcccg gaaacagcaa tgatttctgt tgctgattta
     1561 ccctcgccag ctgccacacc gatgctgcgt tttaccttga tatagtggtg agtaaaattg
     1621 caatacagcg aacggatcag ggatgtgtca ctgttagaaa caatgaccgg atgtccttct
     1681 gatgaccgat gttcaagaac ggatgctagg tgatactggt catcttcagt gaaaccatca
     1741 gtgtgatagc cggaaaacgt accgtcatac ggtggatcgc aatacaccac atcccccgcc
     1801 ttcaacatcg ccagcgtttc atcaaagctg gcgcagataa acgttgctcg ctgggctttt
     1861 tctgcaaatg cgcgaatttc tttttcaggg aaatacggat ttttataatt gccgtaggga
     1921 atgttgaaat gcccgctctt gttatagcga cataaaccac ggtaaccgtg acgattgaga
     1981 tacaggaaat ataccgcttt catgaaatca gtaatttcag ttgagtaatt aaactcctgc
     2041 cttatgttgt aataagccac ctccctgttt gcgatctcaa ataaaactc
//
LOCUS       DAJTNA010000341         2078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTNA010000341 DAJTNA010000000
VERSION     DAJTNA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.348"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(300..1754)
                     /gene="ospC4"
                     /locus_tag="OO161_004224"
     CDS             complement(300..1754)
                     /gene="ospC4"
                     /locus_tag="OO161_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5465038.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSK
                     FNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKAN
                     SGDTMLDNAMKSKNPKMIDFLLKNGAILGKRFEI"
BASE COUNT          620 a          373 c          347 g          738 t
ORIGIN      
        1 gtgtccgtta aactcaggct acctcactcc cgtaaagaca ggagtcaggt tcccgctccg
       61 acataactgc gtgcaatgga ttttgaagga accgtcagcc cattccccgg aggcaaacat
      121 gggctggcaa aagtgtagcg gtattaggca cagctattta ggaaagaggg tccatctcca
      181 tctcattaaa tggaccacgc cagacaattc actgtcatgc tgatgctgca ctgtggggac
      241 agaatcactc atgatgactt cgataatcga cgacattatt atttggccga gcttttaggt
      301 catatctcaa atcgtttgcc taatattgct ccatttttta ataaaaaatc aatcattttt
      361 ggatttttgc ttttcattgc attgtcaagc atagtgtccc cactatttgc tttttgaaat
      421 tttttgttta catcaaccaa tccattattg ataaaatatt ctagcacttt atagttggca
      481 ccatctttac taagtaagta ttcaacatca tacaaattac aatatctatt gttgttcatt
      541 ttctccatct ctgcgacatc ttgttttgta aaattaaatt tagatagtaa ataaagtgct
      601 atatcagctt ttttattgcc taaagaataa gcaagggcct gatgagccat ttttttatca
      661 gtcactttat tgttaatctc ttcataatta gatgctttaa tcgcatcctc caaagatatg
      721 tctctgagtt ttattttttt gaagttatct gtacttagca ttcttggtat atgatactca
      781 agctgaaaca caaagtttat aattctatcc agactaacag gatcaatatt tttgttataa
      841 caaaactctc tgaatccttg atctttactc tttcttataa aatcaataag atgcaaccca
      901 attccaagcc tcatatcttt agtgttaaat attggaacat cagttttccc ctgattacca
      961 tgcacatacc tgctaacttc atccacaacc tctttaaagt tatctaagaa aaatgattgg
     1021 tgctcatggt aaaaaacagg tggaatagca ttatcaaagt gatcggttaa tgtcatatat
     1081 ccatatggag agtcatgatc aatgatatac gcattggcgc caaaatctac agtatgatgt
     1141 gttttattta gtgggagttt ttcttgatga ttggaaattt caaccccaaa aaaaacaaaa
     1201 tcgtggttag agatttcttt aatatcctca gagtatgtat tcttatgtgt ttgtatattt
     1261 tcagactgaa ttttatcaaa ggatttaata ttaagaatat tatttgacag attagagtta
     1321 ctttgatgtc tgaagttaaa attagaatta ataataatag aaagtaagtg acgttcgtct
     1381 gaagaaagag gatgaacttt ttgtattatc tcagagatat agtgcctgga caccttatcg
     1441 agatgctcaa gtgaccgaaa tgatgaccgt gctccagatg gcgcttcaat agaaagattt
     1501 agtgatttga aactggtccc ttgagaaaac attttgctat aactttgcgc tgctatagac
     1561 ttccttaaaa agtttttcac agtattagca cagtgatcta aatgtttttc acttatgcca
     1621 gaagaagatg agtttgttac gcgtaatata tttttttgta atgcttttgt tgctttattc
     1681 ggattcgttt ttccatcaac aagatcaata ttattctgaa cattaatatg atttactgct
     1741 tcaggtattt tcatcccccc cccaattatg tgcgttttta tctgtggtta tttagataac
     1801 attatctaac tggacacttt cttgtatgta ttatagctaa atgaacagat tttaatatgt
     1861 tgtatgcttt tcactctgaa atcccctgaa tttaatcacg tttcctttac tgaaggcaat
     1921 tttaaccgta gtatcaatta ttaacccttg ggcttgatat ccctctcctc ttctgggcac
     1981 gggggatgtc agattactta ttacagaaac caaaagaatt gtaagcgtca tctcagtgtc
     2041 gtctatttca atgtctgatg attggcgatg atagagtc
//
LOCUS       DAJTNA010000342         2070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTNA010000342 DAJTNA010000000
VERSION     DAJTNA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.349"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..1632)
                     /locus_tag="OO161_004225"
                     /pseudo
     CDS             complement(1..1632)
                     /locus_tag="OO161_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
     gene            complement(1984..>2070)
                     /locus_tag="OO161_004226"
     CDS             complement(1984..>2070)
                     /locus_tag="OO161_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HCR5465039.1"
                     /translation="RQMQARRVLQQMSDERLRDIGLRREDVE"
BASE COUNT          541 a          517 c          431 g          581 t
ORIGIN      
        1 ccccccacaa aaaggcaaac agatccgaag cgggataatc tttttgggga gaattgttcc
       61 agttagcaat atatcccgac agggggttat acaccttagg gttcatttca aaaggcaata
      121 gccctttcca gtcccatttt cccgtaccag gaacgggtaa tcgcggatca tggcctgatt
      181 gacgatctgg ataagcacca gtatgaacat aaccaatatt gccgtttaca tcagcatagt
      241 accagttgat ggtcagtgct tgtttcgcta ctcctgccaa tttttggcct tcatctgatg
      301 agttcaggcc agcaaagacg ccacctcttt accatcccat gcgcgggatt tagcgtaagc
      361 cgtttgtgtc gtctggtcag tttggagaat gttgccatgc accgtacgcc agacagtaaa
      421 ggtctctgcc tgaccatttt tcaccgtaat ggtttcctca cggcttaaca ttttcaccca
      481 cttacaatta tgtaagtagt agcctggttt ctctgccgac agccgttcag caaaaatatc
      541 gacatcatcg ccgaaacctg ccgttgatcc ccaggaaatc acaccattat gaccaaaaac
      601 cagcccagga taggcaaatg gtgtattgcc agtgacatca taaccagcac cgtgcagacc
      661 aataccataa gtatacgcag gcgcatacca gccaaactgc ggaccattta ccatgattgc
      721 tttcgcatcc tgggctttgc ttttgccgat cacccacata ttgctggtcg ttggataccc
      781 cgccagacca ttggcaccac cctgtgcaaa ttgtgcagca atagtttccc ggttcttccc
      841 tgctgttaac gccagcagtg cgccatccgc cccttttgct ggtcggtcaa gcattggtgc
      901 aggtaaatcg tagcgtggca acagagctgc tgtttgcgag ttttgctgat taaatttaag
      961 tgggtagcta ctctcttgta cggcaatagt ggttggcgct gatgggttta ccagccattt
     1021 caactgatta aataccgcca tgccttgtga tacaccatat ttatctttta aagccgttag
     1081 cagtgccaga ttatcaattt cgctagtgct atcagagaag cggtttgcca tagtgcccac
     1141 aaatatcatc gcgacatcaa acggttccca gcgcttagga gtaaagccaa atgtattaaa
     1201 ctgttttggt aagagcgtct ctggattggt atttacctta tcaatccagg cattcattcc
     1261 atcagcgtag ccttgcagaa tggacatatt ctctggggaa agggcatcaa tttgcgcccg
     1321 gatagcatcc ggccagtagt tacgacggat atctttatca aatttcacaa aatctttgcc
     1381 aagcacttcc gcgacagtcc cttgagtact gcgacgtgcc atttccatct gaaaaaggcg
     1441 atcttgtgct actacatagc catagccata aaataggtgc catgtatcat tggcataaat
     1501 atgcggcatg ccgtattcat cgcgaacaat ctttatctca cttgacgact gctcagccag
     1561 tgcaggtaag ctccaataat acatcaggga agcagtaaca cagttcacga tcatacgatt
     1621 tctatttttc attgtatcct ctggcaggtg tataattagc gaattgatac tagcaacgaa
     1681 gcttcggtta tctatttgca gagaattgtt tcattatgaa ctgtgagcgc gataactaat
     1741 ttttgataca aaaatttcat taatgtgatc taaaacgaat cgctttattc accgctgatg
     1801 attttgttct attccttttt gtgacagatc ccccggacgc atagatgatg acccccagag
     1861 agacagctca ggtcattatc tgtatgtaaa tcagaaaaat atgttgtaaa cgacttttat
     1921 ggttggattt tgtagcaatt ttgaatgcag acaggcagca tttgccaaca cgccgcccat
     1981 agttcactcc acatcctccc tgcgtaaccc gatatccctc aaccgttcat cactcatctg
     2041 ctgtaacacc cgtcgggcct gcatttgccg
//
LOCUS       DAJTNA010000343         2051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTNA010000343 DAJTNA010000000
VERSION     DAJTNA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.350"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            424..735
                     /locus_tag="OO161_004227"
     CDS             424..735
                     /locus_tag="OO161_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5465040.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLHAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            735..1022
                     /locus_tag="OO161_004228"
     CDS             735..1022
                     /locus_tag="OO161_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5465041.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1560..1727)
                     /locus_tag="OO161_004229"
                     /pseudo
     CDS             complement(1560..1727)
                     /locus_tag="OO161_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            1852..>2051
                     /locus_tag="OO161_004230"
     CDS             1852..>2051
                     /locus_tag="OO161_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465042.1"
                     /translation="MDEKKLKALAAELAKGLKTEADLNQFSRMQTKLTVETVLNAELT
                     DHLGHEKKLHPVVNIISGLCDM"
BASE COUNT          581 a          498 c          493 g          479 t
ORIGIN      
        1 agcaggtacc ggcatccacg ttacagacgg tcccggcaaa agtgcctgac ctgaccccta
       61 ttagcggtta ccagcgcccc accagacccg gtgacatcac cccccggatc atggacgact
      121 gggggcagta atcatgccgg gtctggctgg ctaacacccc attataaggg gtacgaataa
      181 agtaacgggg gtaaggtgaa atctgtcaat cagtaccggc ttacgccggg cttcggcggt
      241 tttactcctg tgccatatgc aacaacggag tgccgccttc catgccgcta acgcggcatc
      301 aaaaaatccc tcttctccag ttgcccccct ccgaactcag agtgtatttt catttattaa
      361 tgaaatttac tgtttgcgca atttttctgt tttagtgtat tttgtcattc tggaggctat
      421 aatatgttta atgtgattac tcacccggcc gcactggatg aacttctaga attaccagac
      481 aatctgcgtg gtcgtatgac cagactgatt gaacggctgg aacaggaagg taacaaaatg
      541 aaaatgcctc acagccgtgt aattggcgga gggctttttg aactgagagt cggggataaa
      601 aatatcgcaa ggacactgca cgcttatgca acgggtaacg aaatctatct gctgcatgca
      661 tttgtgaaaa aaacgcaaaa gacaccaacg aaagctattg aaattgccag gatgcgtctg
      721 aaggagatga actgatgaaa gtaaaaggtg ttccattcag ccaggtaaag caaaaactac
      781 tcagtactcc agaagctgtc aaagggtatg aggatgctga caaagaactg gagatggtgg
      841 aaatgttata cgaaatgaga gaaaaagcgg gattaacaaa gtcagcactg gccgaacgaa
      901 tggggttacg cccgtcagcc atcagtcgcc tggaaagtaa ccctttaggg gccagcatga
      961 aaactttgac aaaatatgct catgcctgtg gcgcaaccat cgatattcac gctgtctatt
     1021 gaggggtccg cttggaattc ggaaaataaa ccacgttaag actatttttt agataaatga
     1081 ctttcaggaa tcgccaagat ggcgggataa cagcggtttt ggtgtcctaa atcgtataat
     1141 tctgtacatc cacgcaaggg aatgtatgga agtaagccag acggttatcg gacggtcaga
     1201 acatttcgta cagcctttac gttcagtgag ttagctggcc tggacgtaac cactcgttag
     1261 ctcaattctc ttagcgtggg aaaatttccg aattccaagc ggaccccttt atccttgccc
     1321 cgctgaatga ggaaacgctg ttccggggga ttatgctgaa cgtcttccgt tcatcgatag
     1381 gaattaaaac cccaaaaaga ttaaaaaaac accacaaaac ggatgtttct tcaacaccac
     1441 ttttgctcat atgaacggaa ccgacgatta aactggatgg ctctgattga ttcagggtat
     1501 gaatggcggt tttttgctcc gtttccctca aaatggacgc cacttaccct ctgcggctct
     1561 cagccgcacc accgcatccg ggccagcggc tcatgcatca ggacctgctc tgccagacgg
     1621 tagccccgct tcagccccgt aaaacgcatc tgactcccgc acagcacgca cttcagcggg
     1681 tcaaccttca gtaacctctg atacatccct ctccagggta agcgtaaacc aggtcctcag
     1741 agaatacata ttctgcggag cgtgcggcaa caacaggacg aacaggggcg gttttcaatt
     1801 tgcagccgtc aggctgccgt ggttcactcc aacaacggag acaggtaaat tatggacgaa
     1861 aagaagctta aagcccttgc ggctgaactg gctaaaggcc tcaaaaccga ggccgacctc
     1921 aatcagtttt cccgtatgca gacgaaatta actgtcgaaa cggtgcttaa tgcggaacta
     1981 actgaccatc tcgggcatga aaaaaagcta catccggtag taaacattat cagtgggtta
     2041 tgcgatatga t
//
LOCUS       DAJTNA010000344         2001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTNA010000344 DAJTNA010000000
VERSION     DAJTNA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.351"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..376)
                     /locus_tag="OO161_004231"
     CDS             complement(<1..376)
                     /locus_tag="OO161_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465043.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTA"
     gene            complement(392..1897)
                     /locus_tag="OO161_004232"
                     /pseudo
     CDS             complement(392..1897)
                     /locus_tag="OO161_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          549 a          493 c          440 g          519 t
ORIGIN      
        1 tcgccgtaaa ttgtacggtg acgtcatttg tcgtgttcag gtcacattga acatctggta
       61 catcactgat ataggggtca aatccaaaat cagcaccaca ggcacaaaga cggctaatgg
      121 tggcggtatc gagggaaaat cccggccata ctttgctgga ggttaagcca atatttaatg
      181 tgacatcata tcgaagcaat ccctcaagac cgtcagcacc gacgaacttt tccccttcat
      241 ccagggcctg gttaataagt tgctgaacaa tgccttcatc ccagtatgcc gatttgcagt
      301 tggcaatcgt aatatccacg cctgtgaagg gatcagtacc ggcaaggctt gcaaataatg
      361 tattaactaa ttccattttt attatccagc tttaaggtag cgtcttatac atcgggacat
      421 aacctacatc ttccaccaaa ctctgtccct gcggcgttaa aaaccactcc aacaattttt
      481 gtgtctctga cgtcgtattt tcccgcgtta ccataaaggc atcgatgata tagggatatt
      541 tgccgttgcg aatgttttcg gcagtcggtg caataccgtt aatcgctagc agttttatat
      601 ttttatcggc gttcatttgt gttgcgtaat aacggaaggt gtaacctatt gcgttgtttg
      661 tattacggta ttcggcaacg actttaatca ttccctccat catacttgcc acttctgttt
      721 cctgcggcga gatcatgcgg acatttttca tgacctggga ttgcatcacc gtctggctac
      781 cagagtcttc cgggcgttgc taggtctgga tctcctgatc gttaccgcca acagtacgcc
      841 agttggtaat tgcaccactg aagatgtcac gcacctgttg ttcagtcagg gaattaaccg
      901 gattatccgc attgacgatg aaaacaaacg cttcacgggc aaaaggcgtg tacagcaaag
      961 taatgcccga ttcctcagcg cgttttttct gcccaccgga aggttgcgcc acaaaaataa
     1021 tatcagcatc acctttaaca atcctgttat acgcatcggg ggtacgagag ctctccagat
     1081 attccctggt gtgaaaatct tccggtataa cgattaaagc ataaaatgca gaggcataaa
     1141 ttgggtacgc cgccgtagca ccatcgagtc gcggccagtt ttgcgtaaac tgaatttgcg
     1201 gtttaccacg cagtggtgtc agttgattat tgagtttatc gggtcgccag gcccaggtat
     1261 caatattttc gcgcacaccg acgccgggat tgagctgttt atataaataa ccctgataac
     1321 cagcgacaaa accaagcatg agaaggataa acaggaggga attacggcaa cgtagcccgg
     1381 atgtaccggt taaaggatgt ctggttggcc ggccgtaccc cagcgaaaaa ctgatatgcg
     1441 cgccaacggg tataatgact atccaccagt actcacctcc gactactgag actaacaaaa
     1501 cgcccagcca ggggagcata agatcagcca accaacccga tcccatctgg tgaaattgat
     1561 aatctgccag atgcagcgcg attatccagc caaccaggat taataaaccg ggtactaaaa
     1621 aaagacattg tcgccagacg gctatttctc ttttttctgg tgcactatcg cctgcccata
     1681 ctcgccccga gaaaaatagc attaatgcgc ccagcgccag cataaaaatg ttgagcagaa
     1741 ggtaacttgt tggcgtgata aggatattac aaacttcaat aataccggca agtccgacac
     1801 ccagcatagc aacaaccgcc gccaaaaatt gcgtcagtat ggggatgccg aaaaaagtca
     1861 ttaccagcga ggacaaaatc cattttctgt tttgcattat tctttccatt ctttttgaat
     1921 ggtgaaatta tactccccga gtccccttgc cccttctgga cacttttccg aaatgatggc
     1981 ggaaaaaaac gggacccttt g
//
LOCUS       DAJTNA010000345         1918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTNA010000345 DAJTNA010000000
VERSION     DAJTNA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.352"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..362
                     /locus_tag="OO161_004233"
     CDS             <1..362
                     /locus_tag="OO161_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5465044.1"
                     /translation="NIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRDVTE
                     EEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGLVDA
                     LEDDDDVQKVYHNVANL"
     gene            complement(813..888)
                     /locus_tag="OO161_004234"
     tRNA            complement(813..888)
                     /locus_tag="OO161_004234"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Asn,seq:gtt)
     gene            complement(989..1840)
                     /gene="yeeO"
                     /locus_tag="OO161_004235"
                     /pseudo
     CDS             complement(989..1840)
                     /gene="yeeO"
                     /locus_tag="OO161_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
BASE COUNT          599 a          446 c          381 g          492 t
ORIGIN      
        1 gcaatatcgg agcggcaggt tctgtcagct atatgtttga caatacgggt gtgattgtat
       61 ttaaaggaac agaccctgac catatttttg aaattttact tgaagctgaa gttgatgttc
      121 gtgatgtgac tgaagaagaa ggtaacattg ttatttatac tgaacctact gaccttcata
      181 aaggaatcgc ggctctaaaa gcagctggaa tcactgagtt ctcaacaaca gaattagaaa
      241 tgattgctca atctgaagtt gagctttccc cagaagattt agaaatcttt gaagggcttg
      301 ttgatgccct tgaagatgac gacgatgttc aaaaagttta tcataacgtc gcaaatctct
      361 aattatattt taaagaaatc tgtctttatg gcagatttct ttaatctcat ataatcatta
      421 taaaaatata atgttcaact cgtcatattg cttataaaaa atccccgttc ccagagaaat
      481 aatatttatt aaaattctag gtcttctttt tctgattacc gaagacaaag tggcaattac
      541 gtatagtttc ccgataaaga cgcgacagcg acatcccgca taaggcattg ttctctttat
      601 ctttgtacgg tacttcatgg aacagagatt ttgaccttgc gaatcgtgat gtctgttggg
      661 ggcaatttgc tcactgaagc gtgagactcg attaaacgca cgaaacacag aaatcaaaaa
      721 aaccggtcac ttttttacaa ggtaaccggg taaaaataat ttttattttt taactgtttt
      781 gagattcata gagatgtctc aaaactaaaa tttggctcct ctgactggac tcgaaccagt
      841 gacatacgga ttaacagtcc gccgttctac cgactgaact acagaggaat cgtgagaacg
      901 aagcgaatat tagcgatgcc caaccacaat gtcaaagcct gttttttaaa tttgaaatcg
      961 tttgctgaaa taatctgcat tttgtcgttt attccgacac agctggcttt ttttcacact
     1021 tttgcggttc gggtcgaggg tatttccata gccaacgtcc agtaaccatt cgccagtaaa
     1081 acagcacggc ccgcacagcc cagtcggcaa acattcccat ccagacacca accacacccc
     1141 agccaagcat gatgcccagc acataaccga cgacaacccg gcaaccccac atgctcaaca
     1201 tagaaaccca catagcgtaa cgggcgtcac gagccccctt aaatccagcg ggcagcaccc
     1261 atgaggcgga ccaaataggc ataaataaag catttagcca aatcagaatc acaacgacat
     1321 gtttaacctg tggatcctgg gtgtaaaacg atgccataac cccggcaaag ggagccgtta
     1381 gccaggcgat ggccgttaat ccaagagtgg aaagccagaa cacatgccgc aactgaatct
     1441 ctgcttgcgc tatctgtcct acacccaacc ttcggcctgt aatgatcgta gaagcagagc
     1501 cgagcgcact tccgggtaag ttgataagag ccgcaattga aaacgcgata aaatttccgg
     1561 caataacact ggtccccatc ccggcaacga acatttgagt taataaccga ccactggtaa
     1621 ataacactga ttcgacactc gcgggaatac caatccccat gacttcccag ataatgctaa
     1681 aattcagcgg tttaaaatag ctctttaacg aaatccttag cgcaggatta aaaccaatcg
     1741 ccaacaccca caaaattgca actgcgccaa tataacgaga aatggttaaa cccaacccag
     1801 ccccgacaaa tcccagtcac ggccaggaga aaaggccgta ggtaatgctg ccaacttact
     1861 gatttagtgt atgatggtga ttttaaggtg cttgcgtggc ttccatttcc atcagatg
//
LOCUS       DAJTNA010000346         1849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTNA010000346 DAJTNA010000000
VERSION     DAJTNA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.353"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            129..1849
                     /locus_tag="OO161_004236"
                     /pseudo
     CDS             129..1849
                     /locus_tag="OO161_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          470 a          517 c          539 g          323 t
ORIGIN      
        1 agcttaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc atcaccatca
       61 ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg ctgaagcggg
      121 tggtgaaaaa tcacccggag acggaagcac tgctctggca gcatgagacc agacatgcgt
      181 acagcgcgca gggactggcg aaccgctgta taccggacag cctgcccgcc gtggaatggc
      241 tgacctacgg cagcggctgg ctggcaggta tgaagctcgg cgacacaccg ctggtggatt
      301 tcacccgcga ccgcctgcac cgggaaacgc tgcgccgctt cggccgttat gaactctcca
      361 ccgcttacac ccctgccggg cagttacaga gccagcacct gaacagcctg cagtatgacc
      421 gcgattacac ctggaacgac aacggcgaac tcatccgcat cagcagcccg cgccagaccc
      481 ggagttacag ctacagcacc accggcaggc tgaccggcgt tcacaccacc gcagcgaacc
      541 ttgatatccg catcccgtat gccacagacc cggcaggtaa ccgcctgccc gacccggagc
      601 tgcacccgga cagcaccctc agcatgtggc cggataaccg tatcgcccgt gacgcgcact
      661 atctttaccg gtatgaccgt cacggcaggc tgacagagaa aaccgacctc atcccggaag
      721 ggggtatccg cacggatgat gagcgcaccc accggtacca ttacgacagt cagcaccggc
      781 tggtgcacta cacgcggaca caatatgcag agccgctggt cgaaagtcgc tatctttacg
      841 acccgctggg ccgcagggtg gcaaaacggg tatggcggcg tgaacgggac ctgacgggct
      901 ggatgtcgct gtcacggaaa ccggaagtga cctggtacgg ctgggacggc gaccggctga
      961 ccacaataca gaacgacaga acccgcatcc agacggttta tcagccggga agcttcacgc
     1021 cactcatcag agttgaaacc gccaccggtg agctggcgaa aacgcagcgc cgcagcctgg
     1081 cggatgccct tcagcagtcc ggcggcgaag acagtggcag tgtggtgttc ccgccggtgc
     1141 tggtgcagat gctcgaccgg ctggaaagtg aaatcctggc tgaccgggtg agtgaggaaa
     1201 gccgccgctg gctggcatcg tgcggcctga ccgtggcgca gatgaaaaac cagatggacc
     1261 cggtgtacac gccggcgcga aaaattcacc tgtaccactg cgaccatcgc ggcctgccgc
     1321 tggtgcttat cagcacggaa ggggcaacag aatggtgcgc agaatacgat gagtggggca
     1381 acctgctgaa tgaagagaac ccgcatcatc tgcagcagct cattcgcctg ccggggcagc
     1441 agtatgatga ggagtccggc ctgtattaca accgccaccg ctattatgac ccgctgcagg
     1501 ggcgatatat cactcaggat ccgattgggc tgaagggggg atggaatttt tatcagtatc
     1561 cgctgagccc ggtgaatagc atggatccat taggattata tgaatttaaa tcaaaaaata
     1621 tagatgatat tggaatattt gcattggcaa tgtgtaatgg agaatcaatt aacgagaata
     1681 aagaatatgg tggactaata tgtaagaagc aaggtgaata tttccccatg aatccgataa
     1741 gttcaaatga taatgatagt gtagacttgc gaaatataaa atgccctgaa ggttcagaga
     1801 gagtaggcga ttatcacact cacggttttt actctgacga taaagggaa
//
LOCUS       DAJTNA010000347         1845 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTNA010000347 DAJTNA010000000
VERSION     DAJTNA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.354"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(233..1333)
                     /gene="chaA"
                     /locus_tag="OO161_004237"
     CDS             complement(233..1333)
                     /gene="chaA"
                     /locus_tag="OO161_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5465045.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1603..1833
                     /gene="chaB"
                     /locus_tag="OO161_004238"
     CDS             1603..1833
                     /gene="chaB"
                     /locus_tag="OO161_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5465046.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
BASE COUNT          570 a          407 c          427 g          441 t
ORIGIN      
        1 aattccggct tggtgtggat actacgtctc aattcatctt cacttcatcc ctgaaatgtt
       61 tgcaataaag agtacattcc ggcttttcaa cagctgttgc agtggttttg tgcaggttgt
      121 ggtacaggct cgcaattctg attaacgagc tgtccaggta gtatgaagaa atacaataag
      181 caggaattta tcttatctcc cccgatttcc gtcatcggag gagatataac tgtcaggcaa
      241 atatcgtcat caaataggcg gcaaacagtg ccagatgcgc tgcgccattg agcacgttag
      301 tacgtccggt ggagaaggag atatggcaca gcactaaaga ggccaccatc accaccattt
      361 ctggcgcacc aagtgcaaac tgtaattcgt tacccgtcat aaaggcaatt agcgtgacga
      421 caggtacggt aagcgaaatg gttgctaaca ctgaaccaaa gaacagattc atcgcgcgct
      481 ggacctggtt gttcaacact gcttttaacg cacctaaacc ttccggcgac agaatcaaca
      541 gagccaccag gaagccagta aaggcgacag gggcattcat gctgtcgagc aatgtctcca
      601 gcgggctggc attcattttg gtgactgcaa taacggcaat cagatggata atcaaccaga
      661 tagcatgcca cacgctgcta tgggcagacg gtttaccgtg atgcgggtcg tcgtcatcac
      721 tgtcatcttc gtgctcgtag acaaacaaac tttgatgcgt tttggtctgg atcagcaaaa
      781 atacgccata cattgccgca gaaattaatg ctaccagtaa cgcctgaccg gttgaaaaat
      841 tcgccgcagg cagagccatt ggaaatacca gtacgattat cgccaggggg aacagcgcaa
      901 ttaaatactg cttgatacca aacagattca tatattgggt ggcaaactta cgaccgccca
      961 acaataatga aaagccaacc agcccaccgg taacaatcat aatgattgaa tagagcgtat
     1021 cacgcattag cgttggcgcg gcgtcgccgg ttgccattaa agctgaaatc aaactgactt
     1081 caagaataac cactgaaagg ctaagaataa gcgaaccgta aggttctccc aggcgatggg
     1141 ctaatacgtc cgcatgacgg acaacactaa aggcgctact taaaatacca ataagcgcaa
     1201 gaagattgat ggcaatgacc actggtagtg tctggctgct tccccacagg aacagcacta
     1261 ccagcgccag aaccgggaaa ataagcgaag tctccttgtg gcgggttttt accgcctctt
     1321 gagcatttga cattatggtt atccctttgc agatgaattt atcgaaaatg taaaaaatag
     1381 gtaggaaaaa taacagaaag tgtctggata tcggtaacat tttacgaatt tttaccccac
     1441 tgtcgatttt ttactcattg ggacataaaa ataagtatta cgtttagaca atgtttgttt
     1501 tagcgtttct taagaacagt ctgacatgaa aattcttatg ttttggcaag agtagatatt
     1561 gttgaccaca cttaatgttc aactttgtaa aaggagtcaa cgatgccgta taaaacgaaa
     1621 agcgatctgc cggaaagcgt aaagcacgtt ctaccgtctc atgcccagga tatctataaa
     1681 gaagcgttca acagcgcatg ggatcaatat aaagataaag acgatcggcg tgatgacgcc
     1741 agtcgcgaag aaaccgcgca taaagtggcc tgggctgctg tgaagcatga atatgccaaa
     1801 ggggatgatg ataaatggca taaaaaatcg taaaaccggt agctt
//
LOCUS       DAJTNA010000348         1842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTNA010000348 DAJTNA010000000
VERSION     DAJTNA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.355"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            500..1617
                     /locus_tag="OO161_004239"
                     /pseudo
     CDS             500..1617
                     /locus_tag="OO161_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          713 a          340 c          259 g          530 t
ORIGIN      
        1 ttaaaaatca caaatgattt aactaatgca ttgagaatga tgacggctcg gtcaacaagt
       61 catcaacaga caataaccac gtaatcatgc ttctctgaca ttttatcgga gaagcaatga
      121 ctctttgtct cagtaccctc ctccttttta aaaggcagat ttcagccgct aatacaacac
      181 atttttaaat acactttaac ctggttcatt attacacatc aaaaacatca caaaaatatt
      241 acttcacaca ctatcggaga acataataac tttttaaaat gagcgctttt tgatagttgt
      301 taacgttttc ctttacgata ctatcactgc tttttttata cacaaatatt accgggtgga
      361 aatgaaaggg aatttaaagg taattatatc aggaagatat aacgcattat atttattgtg
      421 tgtagaagaa aatccattcc tgttctaatt atccaattta aaaaccttag cataaaacag
      481 atgatgaata ataaatgtta tgtcaattga ctttacccca ggtataataa atacatatca
      541 cggcgatatt tataactgca caacaaatac cgataatgtt aaaacgccag acacaccgaa
      601 gtggccttgt gataattggg aagaacaaca acccattaat tccacattct ctgaagaagg
      661 atacaaccct gaacaatttg atttagcgca acaacaactc caacaaatta atgcatgcca
      721 cacgaacacc acatatacaa atgcagacta ctcaaaagtt gtggttcaac ttgtaagtct
      781 tattaataac attgaaacga taagttcgac accacttacg caacagacac aatcaatact
      841 taatcagatt aataacatta gatatgaaaa aaacaaaagt gctgaatgtc gaatcattgt
      901 tgtcgctaat cctaaacccg acaaggcaat cataactaaa attagcgtag aagagggaat
      961 acccataaca ttcagtgtcc aaaccatgtt ttcagatact aattttattg ctgaacaacg
     1021 agctgactta ccaactaata taaaagatat tcaaagcctg tatcaaaaaa tgacgaagtt
     1081 atatattgag cacagtgaaa ataaaaacag gatgaaagtc tttgccggaa ccaactttat
     1141 tgatttcaat atgacaggac agaacctttc cgggttcgtt ctaaccttgt caaggtttta
     1201 tttcgaagat ctacttaata taaatatcac tgacgcaaat ttaggtgaca ctattttttt
     1261 acataaagaa caccctaccc cccaaattat ataaagatgg tcaatatctt gacaaacaaa
     1321 tcgaaggttt attttcaaca ttattaacca ttaatgacaa ctttttacga gcaaaagcgg
     1381 aaattgcttc gacaataatt aaatttttag aggccaggat aaccaatctt tcatacaatg
     1441 acatattaaa atatcagcaa gaattccaaa aacaatgtta taagcaagtt aaagcctcta
     1501 cgacactctc tcgatacaat aaaattcaaa catgggcaga aatgtctgaa tatcagttcg
     1561 aagtatttca attcgagacg cttaacccaa aaaaaatgtc acacacgcct tatttaaaga
     1621 ggccacttcc taatgaaaaa gatataaatt atggggtgga aatagaaata ccatctggca
     1681 aaagaattcg cttatcaaat cattatcaga atataatacc ataatattga ttattagcac
     1741 tttataatta ttgaataaaa atagatttta tgtaatttta aaacaaagga ttatattatg
     1801 gggcgatgga tacttatgcc acgccccaaa atcaatggaa tt
//
LOCUS       DAJTNA010000349         1825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTNA010000349 DAJTNA010000000
VERSION     DAJTNA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.356"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>1825
                     /gene="ehaG"
                     /locus_tag="OO161_004240"
     CDS             <1..>1825
                     /gene="ehaG"
                     /locus_tag="OO161_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HCR5465047.1"
                     /translation="TATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIG
                     ETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISD
                     SSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDT
                     TLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKY
                     IADALDGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYN
                     ASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQ
                     ENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIA
                     LGSSSVSSRVIAKGSRDTIITENGIVIGYDTTDGELLGALSIGDDGKYRQIINVADGS
                     EAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIG
                     IGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVG
                     EFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNT"
BASE COUNT          478 a          459 c          476 g          412 t
ORIGIN      
        1 cactgcaacc agttccgatg ccattaacgg tagccagctt tatgacttaa gcagcaatat
       61 cgccacctac ttcggcggca atgcttctgt gaatactgac ggtgtgttta ccggtccaac
      121 ctacaaaatc ggtgaaacaa attattataa cgtcggcgat gcactggctg cgattaactc
      181 ctcatttagc acgtctctcg gcgatgctct gctttgggat gccaccgcag gtaaattcag
      241 tgccaaacac ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat
      301 ttcagactcc agttctgacg cagtaaacgg ctcacaactc cacggcgtga gcagttatgt
      361 tgttgatgcg ctggggggtg gtgccgaagt caatgcagac ggcaccatca ctgcgccgac
      421 gtacaccatt gctaatgctg attacgataa tgtcggtgat gccctgaatg ctatcgatac
      481 cactcttgac gacgctctgc tctgggatgc ggacgccggt gaaaatggtg catttagcgc
      541 cgctcacgga aaagataaaa ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc
      601 tgctgccagc agcgacgcga ttaacggctc acaactctat accaccaata agtacatcgc
      661 tgatgcgctg gatggtgacg cagaagtcaa cgctgacggc accatcaccg caccgactta
      721 caccattgcg aacgccgagt acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa
      781 cgccctgctg tgggatgaga ctgccaatgg cggtgctgga gcctacaatg ccagccatga
      841 cggtaaagcc agcatcatca ctaatgtcgc taatggcagt attagtgagg acagtaccga
      901 tgcagtgaac ggttctcagt tgaatgcgac gaatatgatg attgagcaga acacccaaat
      961 tatcaatcag ctcgctggta acaccgacgc aacctatatc caagaaaacg gtgcgggtat
     1021 taactatgtg cgtactaacg acgacggctt agcgttcaac gacgccagcg cacagggtgt
     1081 tggcgctaca gctataggtt ataattctgt cgccaaaggc gatagcagcg tagctattgg
     1141 tcagggcagc tacagcgacg ttgatacggg tatcgcccta ggtagcagct ctgtttccag
     1201 ccgagtgatt gccaaaggct cccgtgacac catcataacg gaaaatggca ttgttattgg
     1261 ttacgacacc acggatggcg aactgctcgg tgcattgtct atcggtgatg acggtaaata
     1321 tcgtcaaatc atcaacgtag ccgatggttc cgaagcccat gacgccgtta cggttcgtca
     1381 attgcagaat gcgattggtg cggtcgcaac cacgccgact aaatacttcc acgctaattc
     1441 aacggaagaa gattcactgg cagtgggaac tgactcgctg gcaatgggtg cgaaaaccat
     1501 cgtgaatggc gataaaggta ttggtatcgg ttatggtgcc tacgtggacg cgaatgcact
     1561 taacggcatt gccattggta gcaatgcgca agtcattcat gtcaacagta ttgcgatagg
     1621 taatggttct acgaccactc gtggcgctca aaccaattat accgcctaca acatggacgc
     1681 accgcagaac tctgtcggtg aattctcagt cggtagtgcg gatggtcaac gtcagatcac
     1741 aaacgtcgca gctggttcag cggataccga tgcggttaac gtgggtcagt tgaaagtcac
     1801 tgatgagcgc gtagcgcaaa atacc
//
LOCUS       DAJTNA010000350         1819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTNA010000350 DAJTNA010000000
VERSION     DAJTNA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.357"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..165)
                     /locus_tag="OO161_004241"
     CDS             complement(<1..165)
                     /locus_tag="OO161_004241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465048.1"
                     /translation="MIFFDEEEAEVLRFDNFDDAVECCADLNIPFFVDAGNKKLVFWF
                     VRVDDEGYPEI"
     gene            complement(1002..1475)
                     /locus_tag="OO161_004242"
                     /pseudo
     CDS             complement(1002..1475)
                     /locus_tag="OO161_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1403..1769)
                     /locus_tag="OO161_004243"
                     /pseudo
     CDS             complement(1403..1769)
                     /locus_tag="OO161_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          416 a          510 c          429 g          464 t
ORIGIN      
        1 gatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagct ttttgtttcc
       61 ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat cgtcaaaatt
      121 atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa atcatcttcg tgaagtttta
      181 cgacatagcg gaggagtttg cttctttgag gtgcaggttt tcgtttgcgt ggtttagctg
      241 aacgggccat ataactacct gaaagacaat gatattgtct gtttttataa cggtaatcac
      301 aggccatgac aagccgcagc cgtcaggccg cctactcggg gcggtttaga acgcttattt
      361 agtaccagat cttacttata ataaaggggc ttggcagttt ggataaacta ttgtccttat
      421 tccactaata caaacttttt gtaaagtata ttaaatatac ttcatttcat actgaaatcc
      481 ccggaagtat tcaatcaaac aattacacca taaaaggaag aggggcccca attaagttgt
      541 caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca ttggtactct
      601 ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca ggtccacgtt
      661 ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc gcaggcagta
      721 aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg ctttcgacac
      781 atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc tcacgtccga
      841 ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg atggtttggt
      901 gcctgcgcct gcacgccgac accgaggggc cttccctcat ctcttgtgcc gcatggctgc
      961 acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc atagcaacag
     1021 taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg gcctcgctgg
     1081 atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta gcgtttattc
     1141 agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat cacaccgccc
     1201 tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg ttttccacag
     1261 cagtctcata aaacgtgcat cactgatcct gcggcagacg gctttcatca gcagccggtg
     1321 atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc ccgtgtttcc
     1381 ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg ttcgggccac
     1441 tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt gcacggtaac
     1501 tgatcgggct gaacttccgg gaggcttttg cgctcagttc ctgacggcgc agactggctg
     1561 ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca ggcgtggggc
     1621 accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg ctgacggaac
     1681 tgctgacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc gctgcgggcc
     1741 acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg cggcaacggc
     1801 tttccggctg acggtagtg
//
LOCUS       DAJTNA010000351         1817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTNA010000351 DAJTNA010000000
VERSION     DAJTNA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.358"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(317..1465)
                     /locus_tag="OO161_004244"
     CDS             complement(317..1465)
                     /locus_tag="OO161_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5465049.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWSIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          573 a          464 c          395 g          385 t
ORIGIN      
        1 tggttggcgt agttattaac aacgttgcca aaaccccaga cagtgataaa agacatcagc
       61 gcaatgttgt accatcgcag ctgtttttcc tgaacattcc cagccataga caaatccttc
      121 gccaaaatta taggggcatc tgtaaaacga gggtttatgc gcggcaaata agaagtgcgg
      181 aaatagcgaa atgcaaaatt ataacctgct gcacatttta tttttaaacg aataaaattt
      241 ggaataaagc aagtcagata gaagaaacaa aaaagcagag tgtgaaacag taagttcaca
      301 ctctgcttga attcatttac acgtgagcaa cgggtttcgg cgtatgaccg gcgttgcgca
      361 gcagcatcag caaatagata aacgatacgc tggcgatcat gataaacaat aaattatcgg
      421 agaaattctg cattagcata gcggtaaatg acgggccaag cagacttccc acagtatagc
      481 tcaacagtaa ggcctggttc atcgccacca gttgatgatg ttcaactttc tcgcaagccc
      541 atgccatcgc caccggatat agcgtaaagc cagcggcacc gaggatgaat aacgctgggg
      601 ccatcgccgc ctggctaagc atcgcgatac tgccgagaat gacgacaaag acctgaacac
      661 gcaacaccag cagtcgacca aacttatccg ccagacgtcc aatcgaccat tgtccaagga
      721 tacccgcact gaccagtacc gccatccaga aaccaatgct ggcattgctc acccctttgt
      781 gattgaggta cagcggcatc aggccatata gagatcccag aacgattcct gagataatgc
      841 agccattcac gccaagccgc gcctgacgga gttttagcat tgacgtaatt gacgtcgaat
      901 catggttttc agcctgctga ttcagcacac gcgtaaacaa cagcggtaag atccctgcca
      961 acgtcaaacc tgtaacccac ggcaatacgg acatcagctc ggttgaaact ttgctgacca
     1021 gtaactggcc taaaaacgtt cccacgtaat aaaccatcat atacgcagca agcaaacgcc
     1081 cacggttacg tgacgtccca ctgcacatca gcgcgctctc aacaaccacc caaatcatgg
     1141 cacagccgac gcccgcgaca aaacgccaag ccaaccagct ccagaatcca atcatcaggc
     1201 caaggccggc acagccagcg gcaaaaatga aggaggccag ataatagctg cggttaaagc
     1261 caatgcgctt aatgacatac cctgtcagca atgtaccgac aaggttgccg gtaaaatagg
     1321 atgagctgac aacgcctacc tgccatgtgg acatgtgttc ctgggcgagc caaagcggca
     1381 cgagtgtatt taacaccgca atcgccagag tcaacaaaag caggccagac agcaaaagca
     1441 tgacaggccg ggtatacgtg gacatgaatt aaaaccgtga ggaagttcaa atttcatgcg
     1501 catcatgcca ccgccaaaaa cattgtcaat gggtgaaaat acgcgatatc ccagcggcgg
     1561 tattatcgat ttatattact cattcataac gctaatgctg aagagatgat tagtctcatc
     1621 tgattgtttt tattgaacta acacaaattt catacgcttc gttcagtcga aaaatttcat
     1681 ggtcacagca aactcatatt tcccgtctat gctttcagag caatgcatcg cgtctgatcg
     1741 atttcagacg taccactgac tctcgaaagg agaagaggat gaccaaaccg tatgttcgtc
     1801 ttgataaaaa tgatact
//
LOCUS       DAJTNA010000352         1814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTNA010000352 DAJTNA010000000
VERSION     DAJTNA010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.359"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..334)
                     /locus_tag="OO161_004245"
     CDS             complement(<1..334)
                     /locus_tag="OO161_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HCR5465050.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTG"
     gene            complement(443..1399)
                     /locus_tag="OO161_004246"
     CDS             complement(443..1399)
                     /locus_tag="OO161_004246"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5465051.1"
                     /translation="MFSALDIKTLMQETLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNTKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGRDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            complement(1418..>1814)
                     /locus_tag="OO161_004247"
     CDS             complement(1418..>1814)
                     /locus_tag="OO161_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5465052.1"
                     /translation="FGIEFFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGN
                     LYLRTRFGHGKTENADSLQYNANRTYFKTIRKATLGYEQNIGDDWIAKIEYKHAWEKE
                     HKNFYDPRDEEKYNVLNQDNIYVHAFYRF"
BASE COUNT          530 a          373 c          310 g          601 t
ORIGIN      
        1 gacctgttac agtgaactgc ggattccagt tcatagtcgc aaattgcatt gttaccgcac
       61 ctgctttttc agaataaata agctgataaa taccatctcc cttataatgc attttacgct
      121 ctggcttcat taaccaatag ccttccggga gggttccgga aatgtaaaga cccgcagcaa
      181 ttggccctct ttcatttaca tcagggaggt tacattctga atagcgagga tccgctttca
      241 cgacaccttc ctgactgcca gagggtgaat tgccatcaac gcatcctgaa agaagaccag
      301 tcaacacagc cgcaataagc aatttatttt tcatttaaac tctcaataaa agtagtattg
      361 cttagttaag ctatttctat gtcagtgtta atgttaatgt taatgttaat gttaatgtat
      421 ataaataata cttctcaacg actcactgtt tatatacacc atgctgttta gttagtttat
      481 taaggacagc gagatttttg ttgtattctc gcgtattttt ggctgggtaa ccaaaaaggt
      541 cactcttttc aggacaagaa tggcttacac ctgatttagc cttaattacc gaataactac
      601 caattgatat gtgaccggcg attccgactt gaccatgtgt aataacatgg tcgcctaaaa
      661 ctacgtgtcc tgaaaaaccg cattgtgaaa caatcaggca tttattaccg attatgcagt
      721 cacgtccaat ctgcacttga ttatcaatac gagtgccttg gcctataatc gtatctccaa
      781 gtgtccctcg gtcaatggtg ttgttacaac cgatttcaac atcatcacct atgataactc
      841 taccgatgct atcaactcgg acatatgaat cacgttcatc cgacatatat tcaaaactat
      901 aattaccgat ggagttatta gaatctatga taacgtcatt gccaataata gtaccttctt
      961 taatcactgt attcgcatga atggcaacat tatcacctat ggtcacacag ttcataattt
     1021 taacaccagg cataaaatgg cagttcatac ctatctggca gtgtttacca atgtatacat
     1081 caggtatcgt tgaagtgttt tcttgctcaa acaaacgata cttatgtacc ttaaaatatc
     1141 ttaaaacttt gttaagatta ttcacgttca ggtaatcgac tacaatcttt gccttagtat
     1201 tagaactaat gatctcttcc ggtccgataa ttatatccgc accagtcgtt ggtatatgca
     1261 acaaatctcc aggtgtcata acaatcgaga gtccaccaac taaaggacta tggattggcc
     1321 gaatggtatc aaccctgagt gatggatcac catataaagt ttcttgcatc aacgttttaa
     1381 tatctagtgc gctgaacata ggtttccctc tgtttaatca aaagcgatag aaagcatgaa
     1441 cataaatatt atcctgattg agcacattat atttttcttc atctcttgga tcataaaagt
     1501 ttttgtgctc tttttcccag gcatgtttat attcaatctt ggctatccag tcgtcaccta
     1561 tgttttgttc ataacctaga gtggcttttc gaattgtttt gaaataggtt ctgtttgcat
     1621 tgtattgcaa actatctgcg ttttctgttt tcccgtgtcc gaagcgggtg cgtagataca
     1681 gattacctgc gttttcaaaa gcatatttga taattggctc gtagtaagtt tcggtataaa
     1741 tactacgatc atttatttta ccgtgattaa attcatcgtt atgctttttc tggtagaaaa
     1801 attcaatacc aaac
//
LOCUS       DAJTNA010000353         1807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTNA010000353 DAJTNA010000000
VERSION     DAJTNA010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.360"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            655..882
                     /locus_tag="OO161_004248"
                     /pseudo
     CDS             655..882
                     /locus_tag="OO161_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(917..1057)
                     /gene="ykgO"
                     /locus_tag="OO161_004249"
     CDS             complement(917..1057)
                     /gene="ykgO"
                     /locus_tag="OO161_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCR5465053.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1057..1320)
                     /locus_tag="OO161_004250"
     CDS             complement(1057..1320)
                     /locus_tag="OO161_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR5465054.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1684..1785)
                     /locus_tag="OO161_004251"
                     /pseudo
     CDS             complement(1684..1785)
                     /locus_tag="OO161_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          544 a          333 c          391 g          539 t
ORIGIN      
        1 tgggcttaaa tttatgtttt tttcccgatg agtgtcaata tgtgaacgga aaaggaattc
       61 aatggttaag tcaatattta gaaaaactga aaatattgct tttttgataa gaagaaaaag
      121 acatttggtt tcataaggtt attggcaata taacgttcaa tccttacttg cattttatat
      181 tggtcaggaa ctttgccctg aagtgttagg aatagtctta atgcgctgtg tgatggacca
      241 gtaaaaaaac aagttttaag ggtaagggat agaatataaa actataatga gctgtcattg
      301 aagatgattt gccaatcagt aagaaatttt aatggattaa catgccgcac aaaatggtta
      361 taggtgttca ttacaataag tgatggaaaa ttgaacgggc gtatgtacgt catctgttcg
      421 gtaagccctc aagagccaga ttaagtgaag ttaaacgaag gctgccagct gtcacaggaa
      481 cgatggcctc tttgtcaggg cactgagtgc agttatctgt gcgcgttact ctaactggca
      541 ctcgaacgtc ggcgacgggg ttaccggtgg atcagactgt tcctgtggcg taaacgtttt
      601 tatgttagta ccaacagatt taccacattt atcatcttaa cagtttgagt gaaaatgcag
      661 gctgcgccgc aaagggagga gactgagtgg cgtgttcagt ctaaaagaga gttgatgcca
      721 gcatacaggg gcgaagctgg tcaacaagtc aacattaaga taatggaata ttcgtagaga
      781 aacgtcaggc agttggcctc aaatgaacag gaagaatata tccccaggaa aattaatgtt
      841 ggtgttataa acacaccaac attaatccga tctgattatt aatcagacaa atatcgttaa
      901 tccgtcgaat tttgaatcaa cgttttttct tacgaccctg aacggcctta aaacgtggat
      961 tagatttaca aatcacatat agccgtcctt ttcgcttcac aatctgacag tctggatggc
     1021 gttcttttgc ggtacgcaga gagttaagaa ctttcatcac gccccctttt tcgtgctaac
     1081 aaaacgacca aaacgttggg tgaatcgtgc aacatttcct tctgatgcca ctgttctcag
     1141 cttccctgta tagaacgggt gcgatttaga agagacatca attgtcacgt acggatacgt
     1201 tacgccatcc agctcaatct cacggtctgt tttgatagtc gagccgattt taaagtactc
     1261 atcaacactg gtgtcgtgga acaccacagt acgatactca ggatggatat tgggcttcat
     1321 catttttacc tgttatgtta tagcataacc ataaagatac atgctcatcc ggatcatttc
     1381 aacacccaat ccctgtgacc attagtggta tgtggaattt ccgcaacggg gaagtctggc
     1441 attgttaaca gccgttggcg acgctcccat aaacgcctgg atgagtgatt gatgatggtt
     1501 tcgctgagag tatggatttt tcgacaatgg aggttatctc cactttactg gccgatgatt
     1561 tatcgcagta tgattgatta cctgacagcg tgtattcctc tcccacggat ataataatta
     1621 cttacaaaaa gggagaggat gcatatttta aatatcactg aagtgaacag tttattgccg
     1681 ttattaatag aaatggagaa ataaataggc gtattctaca attgcgacaa aaacaacgat
     1741 attaattagt ttatgactga tttattgtac tttattctct ttcattggta cttcctcgct
     1801 ttaaaaa
//
LOCUS       DAJTNA010000354         1758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTNA010000354 DAJTNA010000000
VERSION     DAJTNA010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.361"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..99)
                     /locus_tag="OO161_004252"
     CDS             complement(<1..99)
                     /locus_tag="OO161_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HCR5465055.1"
                     /translation="MLKQRRNFRTGTERHANRFTTSASRSNIRYSLS"
     gene            complement(114..392)
                     /locus_tag="OO161_004253"
     CDS             complement(114..392)
                     /locus_tag="OO161_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5465056.1"
                     /translation="MAKVLNTHEQADFERLAAFYPYRDEHGLPVLEESLKDYAKRTNQ
                     AVNTVKRQADRGSIPINQDEKNSRRTVNLFALFLKTIRSAEKYVQMTK"
     gene            489..797
                     /locus_tag="OO161_004254"
     CDS             489..797
                     /locus_tag="OO161_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5465057.1"
                     /translation="MLIGEKIRVIRESEDLTREEFCGLIDVPIGTLRRYETGRIENIG
                     GEVLIKIVNHPRFFKYMNWLMTGKTNEAAGQISPSLSPDGPENTSSSQKSRKTGTQPG
                     "
     gene            886..>1758
                     /locus_tag="OO161_004255"
     CDS             886..>1758
                     /locus_tag="OO161_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5465058.1"
                     /translation="MCPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALHHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMI"
BASE COUNT          447 a          380 c          458 g          473 t
ORIGIN      
        1 actcaggctg tagcggatgt tgctgcgtga tgcactggta gtgaaacggt tagcgtggcg
       61 ttctgttccg gtacgaaaat tacggcgttg cttcagcata aaatgacacc tcgttatttt
      121 gtcatctgca cgtatttttc tgcgctcctg attgttttca ggaaaagagc gaagagattt
      181 actgtgcgtc ttgagttctt ttcatcttgg ttgatgggaa ttgaacctct gtcagcctgt
      241 cttttcactg tgttaacagc ttggttggta cgctttgcgt aatctttcag gctttcttca
      301 agtactggta acccatgctc atcgcggtag ggatagaacg ctgctaaacg ctcaaagtct
      361 gcctgttcgt gtgtgttcag gacttttgcc atgtgtgata acctgcgcta tctgtggttg
      421 tttgtgactt ggtgtactta taagtacacc ttgtgcgcaa gcttagtgta cttataggga
      481 cactgtcaat gcttatcggt gaaaaaatta gagtgattcg tgaatcagag gatttaacgc
      541 gcgaagaatt ttgcggcctg attgatgtgc ctatcggcac tttgcgtcgt tatgaaacgg
      601 ggcggattga aaacataggg ggcgaagtgc ttatcaagat tgttaatcac cctcgctttt
      661 ttaagtacat gaattggctt atgacgggaa aaacaaatga ggctgctggg cagatcagtc
      721 cctctctctc ccctgatggg ccagaaaaca catcgtcttc tcaaaaatcc cgcaagactg
      781 gtacacagcc cggctaatca tagaacgctg ggggcatggt ggtcttgtaa cgctggggtt
      841 tcacgaatga gcataaaatc aattccggga gggtatcttc ttgacatgtg ccctgagggg
      901 cgtaaaggca aacgcattcg caaaaaattt aaaacgaaat cggatgcagt tttatatgag
      961 cggtgggtgc tggcgcaaca gcataacaat gagtggaaag gaaactctat tgatcgccgt
     1021 ccactgtcag tgcttattga cttgtggtgg aaataccacg gccagctaat gaagtcaggg
     1081 cataacacgc gccttaaatt gctgcgcttg agtgaagcaa tggatgaccc gtgcgtgcat
     1141 aaacttaata caacgatgct caccgagcta cgtgtgtcca ggatagagca ggggatacag
     1201 cccagcacca taaatcgaga gattggggcg ttaagcgcga tgtttaccgc actcatctca
     1261 tccggccatt ttcttaacga taaccccgtt caaggcctta aaggaatgaa ggttaacgag
     1321 cgcgaaatgg gatatctgag taagtctgaa tgtgttcagt tgctggatgc actggctgaa
     1381 aatcccgatg aacgactggc tgtcgaaatc cttctgtcga ccggggcgcg atggggcgag
     1441 gtagcggcac tggagcagcg ccgtgttctt cattgtcgaa tcactttttc aaaaacgaag
     1501 aacagcaaaa accgtaccgt tcctatttct gaaagcctgt ttgaaaagat caaaaaacgg
     1561 ggcgggaaac tggtgtttcc gacgctggat tattcattgg ttcgcgatgt catcaaaacg
     1621 gtcgcacctg atgttcctga cggccaggct gttcatgcgc tgcaccacac cttcgccagt
     1681 catttcatga tgaacggcgg caatattctg acgctccaga aaattctggg gcacgcaaag
     1741 attcagacaa cgatgatt
//
LOCUS       DAJTNA010000355         1748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTNA010000355 DAJTNA010000000
VERSION     DAJTNA010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.362"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..357
                     /locus_tag="OO161_004256"
     CDS             <1..357
                     /locus_tag="OO161_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465059.1"
                     /translation="AITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDNYIIQTL
                     DVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDC
                     WQILFFSPDHFNNDFY"
     gene            379..1533
                     /locus_tag="OO161_004257"
                     /pseudo
     CDS             379..1533
                     /locus_tag="OO161_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
BASE COUNT          630 a          311 c          274 g          533 t
ORIGIN      
        1 gcaattactg cattgaattt ctatcaaggc gatgttcctt ctgtcatgat ccgaatccaa
       61 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga taattatatc
      121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
      181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
      241 acccaaaaag aagccatctt aacagcccct acgcacttta ataaaacctg cattacttca
      301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
      361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
      421 ttagtccgtc gaaaaatgaa tgaccttcct gaaatcccca tagaaaataa tctcgaagca
      481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
      541 gcgttacaac caggtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac
      601 ttacaaaatg taattcttta taatgactcg tttcttcgtc tggatttttt aggttataac
      661 gcccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
      721 aaccaggaaa tatctacagg caatatcaat gctaattgtt cattgaaaga tatttacaat
      781 gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc
      841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
      901 aatactattc taaatccttt taaattggat tcaacaaatt ttactaatgc caacatgttt
      961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
     1021 ataacgcctg ttttaataag tattagcgat atgtattctg aagaaaaaat caagtttgtc
     1081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
     1141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
     1201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
     1261 aagatgaaag aattctcatc aaaaaaatat atgattacat cgatataatc attgaagatt
     1321 atatctgctc gtcccccaaa ttaattatac tatggattta aacatagaag actccagctc
     1381 agaagagtta ataccctcga gaaagacgaa aattcttcgg acaatggctt tgaagtcgaa
     1441 gaatataacg catatgaagc atataactca gagaatctat attttaccag agaggaatat
     1501 acgtataatt acgacctttt aaatgcaata tagacttaat aacttcttac aaaatctata
     1561 attaataccg tagatattca ttcatcttac cctgtgcacc aaaagcaaaa actcactgac
     1621 gcgggtttga aactgatggc aaccgcaaaa atgcctgatg cgctacgctt atcaggccta
     1681 cgccatctct gcaatatatt gaatttgcgt gcttttgtag gcaggataag gcgttcacgc
     1741 cgcatccg
//
LOCUS       DAJTNA010000356         1724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTNA010000356 DAJTNA010000000
VERSION     DAJTNA010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.363"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            54..794
                     /locus_tag="OO161_004258"
     CDS             54..794
                     /locus_tag="OO161_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5465060.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     IRLENKGNNPLLVQSWLDTGDDNAEPGNITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGAAIEGNASL"
     gene            829..1724
                     /locus_tag="OO161_004259"
                     /pseudo
     CDS             829..1724
                     /locus_tag="OO161_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          498 a          370 c          364 g          492 t
ORIGIN      
        1 atttctattt ttatattatt ccctgtttta attaactctg tcagggatgg tttatgtttt
       61 ttaacaccaa acatacaaca gctttatgct tcgtaacctg tatggctttt agttcatcat
      121 ctattgcgga cattgtcatt tcgggtactc gcgtaatata taaaagcgat caaaaaagtg
      181 tcaacatacg tctggaaaat aaagggaata acccgttgct tgtccagagt tggttagata
      241 ctggcgatga caacgctgag cctggcaata ttacagtccc ttttactgcc acgccgccag
      301 tatcgcgtat tgatgccaaa cgtgggcaaa caatcaaatt aatgtacaca gccagcacct
      361 cactgcctaa agacagagag agcgtgttct ggtttaacgt actggaagtt ccaccaaaac
      421 cagatgcaga aaaggtcgcg aatcaaagtc tgctgcaact ggcatttcgc acacgtataa
      481 aacttttcta tcgcccggat ggattgaagg gaaatccctc tgaagccccg ttagccctga
      541 agtggttctg gtcaggttca gaaggtaagg cgtcattgcg agtgaccaat ccaacccctt
      601 actacgtctc ttttagcagt ggtgatttag aagctagcgg taaacgctat ccgattgatg
      661 tgaaaatgat tgcaccattt agtgatgagg tcatgaaagt cactggcctt aatggcaaag
      721 caagttctgc aaaagtacat ttttacgcca ttaatgattt tggtgccgcc attgaaggta
      781 atgccagtct gtaatcaggc aaggatataa ttccgcagga agcataacgt gactataaaa
      841 ccaactaaaa attatcataa tcatctgacc cgtatcgcca cgttttgcgc gctgctgtat
      901 tgcaatagtg ctttctgtgc tgaacttgtt gaatatgacc ataccttcct gatggggcag
      961 aatgcctcaa atattgatct cagccggtat agtgaaggta accctgctat accgggtatg
     1021 tacgacgtca gtgtttatgt caacgatcaa cctattatta accaaagcat tacatttatt
     1081 gaaattgaag gaaaaaaaac gcacaagcct gtatcacact taagaattta ttgcagtttc
     1141 atataaattc tcccgatata aataacgaaa aagccgttct gcttgccagg gatgaaacac
     1201 tcggcaattg ccttaatttg acggaaatta tccctcaggc ttctgttcgt tatgacgtta
     1261 acgagcaacg tctggatata gacgttcctc aggcatgggt aatgaaaaat taccaaaact
     1321 atgttgatcc atcgttatgg gaaaatggca ttaatgcggc catgttgtca tacaacctca
     1381 acggatatca tagtgaaact cctggtcgaa gaaatgacag catttatgct gcttttaacg
     1441 gtgggatgaa tttaggtgca tggcgactgc gtgcctcagg caactacaac tggatgaccg
     1501 attctggcag taattatgat tttaagaatc ggtatataca gcgtgatatc gcctcgctgc
     1561 gttctcaact cattcttggt gagtcttata caacgggcga aacctttgat tctgtcagta
     1621 tccgcggcat tcgtttatac agcgacagcc gcatgttgcc tccgacatta gcgagttttg
     1681 cgcctatcat tcatggcgtt gctaatacca acgccaaagt cact
//
LOCUS       DAJTNA010000357         1720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTNA010000357 DAJTNA010000000
VERSION     DAJTNA010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.364"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO161_004260"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO161_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5465061.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1495..>1720
                     /locus_tag="OO161_004261"
     CDS             1495..>1720
                     /locus_tag="OO161_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR5465062.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCA"
BASE COUNT          490 a          290 c          408 g          532 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattctagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaattttc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttattgacg aaaacaggaa tcgtgttcgg tctctttttt atctgttaaa agccagaagc
     1381 atttccttcg ctgactttat agtcaaccat aacacacact ctactgtctg agtccagcgt
     1441 tttttaacat tcttgttaag attatgtgat ctttagcgcg ggaggaaaat attgatgaaa
     1501 cagcctgcgc ccgtttatca gagaattgcg ggtcatcaat ggcgacatat ctggctttct
     1561 ggcgatatac acggttgtct tgagcagttg cgccgcaaat tatggcattg tcgttttgat
     1621 ccgtggcgag atttacttat ctcagtggga gacgttatcg atcgtgggcc gcaaagttta
     1681 cgttgtctgc agttactgga acaacattgg gtttgtgcgg
//
LOCUS       DAJTNA010000358         1684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTNA010000358 DAJTNA010000000
VERSION     DAJTNA010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.365"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..84
                     /locus_tag="OO161_004262"
     CDS             <1..84
                     /locus_tag="OO161_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HCR5465063.1"
                     /translation="ANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            109..1684
                     /locus_tag="OO161_004263"
                     /pseudo
     CDS             109..1684
                     /locus_tag="OO161_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          443 a          377 c          423 g          441 t
ORIGIN      
        1 gctaattctg cgggaggcca aatgacctgg cgatttatta atgattacgg cgcagtcagt
       61 gacccgataa aaatgacgct gtaagcaagg gataaggata ggcgtattat gatgacgacc
      121 agaatagtgg ttggcctcac ggcagggacg tgtctgattt tctcgcaaaa cctgatggcc
      181 gaggtcagtg tattcaatcc ggcgcttctg gaaatcgacc atcaatccgg agtcgatatt
      241 cgccagttta atcgggcaaa cctgatgccc ccaggtgttt atagcgttga tatttttatc
      301 aacggtaaaa tgtttgaacg tcaggatgtg acatttgttc agaataatcc agatgctgat
      361 ctgcacgctt gctttattgc cattaaaaaa acactgtcct cctttggcat aaaagttgat
      421 gcgctaatga tgtggatgag acggtttgcc tcgatcctgc tccacgtatt gaaggctcat
      481 cctggcagtt tgacagtgat aaattgcagc tgaatatatc cattccccaa atctacatgg
      541 acgcgatggc ttatgattac atcagcccca cgcgttggga tgaggggatt aacgcgctca
      601 ccatcaacta cgatttttct ggttcacata cactacgttc agattatggt tcacaagaga
      661 cagataccag ttatctcaat ctgcgcaatg gactgaatat tggaccgtgg cggctacgta
      721 attacagtac tttaaacacc agcgatggcc gtgcggaata caactccatt agtacctgga
      781 tacagcgcga tattgccgcg ttaagaagcc agattatgat tggtgatacg tggacggcga
      841 gcgatatttt cgacagtacg caaattcgcg gcgcgcgttt gtatactgat aacgatatgc
      901 tacccgccag ccagaatggc tttgctcctg tggttcgtgg gattgcaaag tccaacgcca
      961 ccgtcatcat tcggcagaat ggctacgtga tttatcagtc tgccgttcca gaaggtgctt
     1021 ttgagatcac cgatctcaac accgccagta caggtggcga tttggacgta accatcaaag
     1081 aagaagacgg tagcgaacaa cgattcaccc aaccttatgc ttcattggcg attcttaaac
     1141 gtgaaggtca gacagatgtt gatgtcagcg tgggtgaatt gcgcgatgaa gacggattta
     1201 caccggacgt ccttcaggcg caaatacttc atggtttttc ccacgggatc actttatatg
     1261 gaggtatgca ggctgctgaa aattatggtt ctgcagctct gggtgtcggt aaagatcttg
     1321 gcgctttggg cgcaatttct ttcgatgtga cacatgctcg tgcgaatttt agccatgatg
     1381 atacagaaac gggtcagtca tatcgctttc tctattcaaa acgatttgac gacacagaca
     1441 ctagcttgcg cctggttggc tatcgttact ccaccgaggg ctactatacc ctcaatgagt
     1501 gggcatcgcg gcgcaacagc cctgaagact tttgggaaac aggtaaccga cgtagtcgcg
     1561 tggagggaac gctaacgcag tcgttgggga gagattatgg caatttatac ctgacattaa
     1621 gccggcaaca atactggcat accgatgatg tcgaacgatt aatgcaattt ggctacagca
     1681 gtag
//
LOCUS       DAJTNA010000359         1643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTNA010000359 DAJTNA010000000
VERSION     DAJTNA010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.366"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..384
                     /locus_tag="OO161_004264"
                     /pseudo
     CDS             1..384
                     /locus_tag="OO161_004264"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            362..892
                     /locus_tag="OO161_004265"
     CDS             362..892
                     /locus_tag="OO161_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5465064.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQTNLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            899..1132
                     /locus_tag="OO161_004266"
     CDS             899..1132
                     /locus_tag="OO161_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5465065.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSERTERLSQLYSENRR"
BASE COUNT          445 a          367 c          501 g          330 t
ORIGIN      
        1 ccgaacatgg agctacagac cggaaaacgc ctccagccgt actacgaccg cgcagacagg
       61 cctagaattg atgcctggca gacgctggtt aatcaccatt acgggctgca tgacccgaac
      121 gccccggaga accgcaggac gctgacactc cctgataatc tgccggaaac gaaacaggcg
      181 attgctgagg gcgttacacg cgggatagac gcgctttacc atgccggaga gataaaaggc
      241 cgtcaggatg tgattcaggc gctcacagag gcgggacttg aagtggtcag ggtgacgcga
      301 agcagtatca gcattgcaga cccgaacggc gggaagaata tcagactgaa aggagcattc
      361 tatgagcaat cttttacaga cgggcgcgga gttcgagaaa aagctgaaag agagagccga
      421 atctacagag aaaatgctga acgacgagtt caggaagctc gaagaatctg taagcaaggc
      481 tgtgacatca aacgagacga aaatcaaaga cgctatagcc ctgttcacag cttcgacaga
      541 ggaatcactg aaaaaacacc gggaaggggt gaaagaggcg atgatgcagc acaggaagga
      601 cgtgttaaag ctggcaggga atacgggcat gatgttactg gggatggtca ttttcctgtt
      661 taccgtgagt ggcgggacgc tctggtatct tggcgggatg atacagacga acctggaaga
      721 aatcaggaag cagaacgaga gtctggagaa gctgaacgcg aagacatggg gcgtgcgtta
      781 tcacgagggg agcaacggac gttttctggt gttgcccgag gggatgaaag ccgaaacgaa
      841 ctggacgatg gaaaacggga aacagaatgc cgtaaggctg gtacaggagt aacagagcat
      901 gacggagctg gaaagacatt tgctgaacgc attagagcaa ctgcaacagg actatatgca
      961 gcggctgaac gaatgggaga gcgccttcgt ggaattgcag aaaatgtttt cgcttacgca
     1021 acgggacaac gcgatgctga acgagcgggt catgcagttg agtcagcagg tgcagcactt
     1081 gagcgagcgg acagaacgct tgagccagtt atacagcgag aacaggagat aagggacgaa
     1141 cggctgatac aggaatgcga acatgcgtta tccctggagc gtgagcgcca gctggaaata
     1201 caggaacgga cactggatgg cccttcgctg ggatggtaac cagagacagg ctaaaatgtt
     1261 ggccgcgcgt ttccggtgca ttttttggga taatggttag ttgggggcac cgggaacgcg
     1321 cttcagtcag accttgctga aaacacagga acgcgttaaa acgcatacgg gcgcgattct
     1381 ggtgtgtctt ttgttgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac
     1441 ttctgttttc ccgctcaggt ccccgtcttc ttcgcttccg aaagcatgaa aaaaatccgt
     1501 ttcagctgtc tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt
     1561 ggttaaaatt taccgggtgc gatcgcggca gtttttcggg gggtttgttg ccatttttac
     1621 ctgtctgctg ccgtgatcgc acc
//
LOCUS       DAJTNA010000360         1641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTNA010000360 DAJTNA010000000
VERSION     DAJTNA010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.367"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..491)
                     /locus_tag="OO161_004267"
     CDS             complement(<1..491)
                     /locus_tag="OO161_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5465066.1"
                     /translation="MKVNKKRLAEIFNVDPRTIERWQSQGLPCASKGSKGIESVFDTA
                     MAIQWYAQRETDIENEKLRKELDDLRAAAESDLQPGTIDYERYRLTKVQADAQELKNA
                     REDGVVLETELFTFILQRVAQEISGILVRVPLTLQRKYPDISPSHLDVVKTEIAKASN
                     VSA"
     gene            879..1073
                     /locus_tag="OO161_004268"
     CDS             879..1073
                     /locus_tag="OO161_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR5465067.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            complement(1267..>1641)
                     /locus_tag="OO161_004269"
     CDS             complement(1267..>1641)
                     /locus_tag="OO161_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5465068.1"
                     /translation="TYKEQRDKKVSELEQANATITDMQQRQRDVAALDARYSRELADA
                     RAENETLRADVAAGRKRLRINATCPGTVREAPTTSGVDNATGPRLADTAERDYFTLRE
                     RLMLMQKQLEVAQEYIRTQCLK"
BASE COUNT          436 a          434 c          368 g          403 t
ORIGIN      
        1 ttagctgaaa cattggaggc tttcgcgatt tcagttttca ccacatcaag gtgtgatggt
       61 gaaatgtccg gatatttacg ctgtaatgtc aacggcacac gcacaagtat ccccgaaatc
      121 tcctgtgcca cacgttgcag aatgaaggta aacagttcag tttccagcac tactccgtct
      181 tcacgggcat ttttcagttc ctgcgcatct gcctgcactt ttgtgagccg gtagcgttca
      241 tagtcaatgg tgccgggttg taaatctgac tccgctgccg cacgcaaatc gtccagttct
      301 ttgcggagct tttcgttttc gatatcagtt tccctctgcg cataccactg aattgccatg
      361 gcagtatcaa atacagattc aatgccctta ctacctttgg aggcgcaagg gagtccctga
      421 gactgccagc gttcaatcgt ccgcgggtcc acgttgaaaa tttcggcaag cctcttttta
      481 ttaaccttca tgaaaacaac ccattatcaa atacaaggcc cgacatgaaa acgccagaaa
      541 aaggcatttt cggacacttt catgtcggac ctttacggat gcaatattaa aaaaaacaaa
      601 aagttatatt cgagaagtac cgacacgatt ttccctgaaa aattttcata aatagtgaaa
      661 aaccgcgagg tcgccgcccc gtaacctgtc ggatcgccgg aaaggacccg caaaatgata
      721 ataattatca tctacatgtc acaacgtgca tctacgccat caaactacgt caaataatca
      781 attatgacgc aggtatcgta ttaattgatc tgcatcaact taacgtaaaa acaacttcag
      841 acaatacaaa tcagcgacac tgaatacggg gcaacctcat gtcaacgaag aacagaaccc
      901 gcagaacaac aacccgcaac atccgctttc ctaaccaaat gattgaacaa attaacatcg
      961 ctcttgagca aaaagggtcc gggaatttct cagcctgggt cattgaagcc tgccgtcgga
     1021 gactaacgtc agaaaagaga gcatatacat caatccaaag tgatgatgaa taaacatccc
     1081 ggtttcttcc accatcgcac cggacaggcg actatgaggg gacaacgccg cgctccgtta
     1141 acgcggtaaa ccccggtgtg tatcgttttt gattatcccc gcacactcgc gcagaggagt
     1201 ctcccggtcg ggctgcggtc tctgttaatg cggggatacg gcgacaatac cgcgcatcag
     1261 caaaacttat ttcaggcact gagtgcggat atattcctgc gccacttcca gctgcttctg
     1321 catcagcatc aaccgctctc tgagagtgaa ataatcccgt tcagcggtgt ctgccagtcg
     1381 ggggccggtt gcattatcca cgccggaggt ggtgggggct tcacgcacgg taccggggca
     1441 ggtggcgttg atccgcaggc gcttacgacc agcggcaacg tcagcgcgca gagtttcatt
     1501 ttcagctctc gcatcggcta attccctcga gtatctggca tcaagtgcag caacatcacg
     1561 ctggcgctgc tgcatatcag taatggttgc atttgcctgc tccagctcac tgactttttt
     1621 atcgcgctgc tctttgtagg t
//
LOCUS       DAJTNA010000361         1614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTNA010000361 DAJTNA010000000
VERSION     DAJTNA010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.368"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..238)
                     /gene="fimC"
                     /locus_tag="OO161_004270"
     CDS             complement(<1..238)
                     /gene="fimC"
                     /locus_tag="OO161_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5465069.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGV"
     gene            complement(275..814)
                     /locus_tag="OO161_004271"
     CDS             complement(275..814)
                     /locus_tag="OO161_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5465070.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(879..1427)
                     /gene="fimA"
                     /locus_tag="OO161_004272"
     CDS             complement(879..1427)
                     /gene="fimA"
                     /locus_tag="OO161_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5465071.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
BASE COUNT          407 a          433 c          347 g          427 t
ORIGIN      
        1 ttacaccatc ggcattttcc atccatgatt gaattaaata ggtactattt tcatcattat
       61 ttgtcacggc aagttgcact tgtttttgcc ctgccggata aattacgcga gtcgcaccta
      121 aggccactcc cgcttcagcg cgtccggcaa ccatcattgc catgaacatc aggatacctg
      181 ccagcaagca gaatgttatt tcctgcgatt tccttacatt ggcgttttta ttactcactg
      241 tcctgttcct gttatcacat tatctgctga acaattactg ataggttaaa gagaaccagg
      301 cctgggcatt ggcgatgccg ccagtaaccc aacgcccggt agcacgatat ttggcgatga
      361 aatgtagcga agtagagcct gagtaaagcc gtttccagtt tactggagga cgattaatcg
      421 gtacgaggtt tccttcatca tcaaacaacg ctacgccaat attggtggct atccctggcc
      481 cctctcccac ggaaagcaca tccggatttt taccatccgc gacaccgtga aacgccacac
      541 ccacacgttc actcaccacc gtgctacatt cccgtaaatg aataacaaaa ggcaccggtg
      601 cgctatcttc ccctaccgca tgaaaccggt tactgctgat ttgccccata ttgaccgtca
      661 tttgtttatc accggcttca atccggcaag tttccgcaat aatgacgccc tgaaattgca
      721 tatttccgcc gggcagcgtg gtattccatt tatttccggc cagagcaaac attggcagca
      781 acgacgctaa tagaaataat ctcttccttt tcatcgttat ttttatcgca caagggtggg
      841 catccctgcc cgtaatgacg tccctgaacc tgggtaggtt attgatactg aaccttgaag
      901 gtcgcatccg cattagctgc gccaggggtt gcgacaccgg ttgcataata acgcgcctgg
      961 aacggaatgg tgttagtacc gttattcagg gttgtttgct cactgaatgt cgcaccatcc
     1021 agcgtcagcg cagcacccgt tctgtccagg atctgcacac caacgtttgt tgcgctaccc
     1081 gcagctgaac tctgcagagc cagaacgttg gtatgacccg catcaatcgc cgtacctaaa
     1141 aaggcaactg cggctttaga tgcaacattg gtatcgcaat cattcagctg aatgttaaaa
     1201 ccgacagcag aactggttgc tccttcctgt gccagcgatg cggtacgaac ctgtcctaac
     1261 tgaacggttt gatcaacaga gcctgcatca actgcgcaag cggcgttaac aacttcccct
     1321 ttaaagtgaa cggtcccacc attaaccgtc gtggcagcgg ccagagccgc tgtagaactg
     1381 agggacagag ccgacagaac aacgattgcc agagttttaa ttttcatgct gctttccttt
     1441 aaaaaaacta tttctaaatc gacatgggca gtcgttctgt acactttgtt ttgtcaacga
     1501 gtttgactgc caacactgca cagttttccc ccaaaagatg aaacatttgg ggccattttg
     1561 actcatagag gaaagcatcg cgcacaaact ttttcagttt atttgttggc ttaa
//
LOCUS       DAJTNA010000362         1613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTNA010000362 DAJTNA010000000
VERSION     DAJTNA010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.369"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            168..1010
                     /locus_tag="OO161_004273"
     CDS             168..1010
                     /locus_tag="OO161_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5465072.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1013..>1613
                     /gene="capU"
                     /locus_tag="OO161_004274"
     CDS             1013..>1613
                     /gene="capU"
                     /locus_tag="OO161_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5465073.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGH"
BASE COUNT          487 a          285 c          350 g          491 t
ORIGIN      
        1 gctaaattta gtgtgccacc tcaaggttct tagtgagccg tttttaaaag atatgataat
       61 atattattgt ttataaattg ttgatgtttg atttagtagt atataaagtt caccaccttt
      121 attaggcttt ccttttatct ctacttactg ggtgccagaa actttaaatt ttaaacggag
      181 ggggcatttt gtttaaagca aatcatgttc cggtattaat gtatcatcat gtcagccatt
      241 gcccaggact ggttacactt tctcccgaga cattccggaa acaaatgaaa tggttggctg
      301 aaaacaactg gaaaacactg tcatcagatg aactggagtt tttttatcgg ggcggaaaac
      361 ttccccgtaa aagcgttatg ttgacatttg atgatggata ccttgacaac tggtttcagg
      421 tatatccttt attaaacgag tttaatctca aggcacatat ttttttaatt accagtttta
      481 ttggaaatgg tccggttcgt cactctcctg gaaaggaata ttctcatcgc gattgcgaac
      541 atcaaatagc aaccgggaat gctgataatg ttatgcttcg ctggtctgaa gtgaacgaaa
      601 tgttgcaatc tggtctggtc gaatttcatg ttcatacaca tactcataca cgttgggata
      661 aaaagttcac ttcacgagaa gaacagtgta aacatcttcg gcaggactta ttgagcggga
      721 gagaatattt aaaaaaaatg acaggaaaat gcagtaaaca tttgtgttgg ccggaaggtt
      781 attataacaa ggattatatt cagatcgctg aagaacttgg attccattat ttatatacaa
      841 cagaaagaag aatgaatgct cctgctaaag gaactacacg gataggaaga ataagcacca
      901 aggaacggga aaattgcgta tggctgaaaa gacgactttt ttattataca acaccgtttt
      961 tttcatcgtt gctggccctt cataaggggc caagattacc ggatgactaa taatgaatat
     1021 actatttacg gaatcatcac cgaatatagg tggtcaggaa ttacaggctg ttgctcaaat
     1081 gaaggccctg aagaaaatgg ggcattcagt tctgcttgtc tgcagggaga acagcaaaat
     1141 tgcttttgaa gccagtaagt ttggaattga tatcacattc gcgttatttc gaaacagtct
     1201 tcacattcct actgtatgga gattactcgg aatagttcat agttttcagc ccgatgcaat
     1261 cgtttgtcac agtggacatg atagtaatat tgttggtttg gtacggtttt ttacctggaa
     1321 acatccattc agaattatca ggcaaaagac atatttgaca cgaaaaacaa aatttttttc
     1381 aataaatcat ttttgcgatg aggtgattgt tcccggaaca aatatgaaga cacatctgga
     1441 gcaggaagga tgtcgaactc gggttactgt tgtgcctcct ggctttgatt tccaggaatt
     1501 atacgttgat tcccgaaaca gtttgcctcc gagtgttctt tcctggctgg cgtcccgaag
     1561 aggatgcccc gtaattgctc aggtaggaat gttgcgcccg gaaaaagggc acg
//
LOCUS       DAJTNA010000363         1609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTNA010000363 DAJTNA010000000
VERSION     DAJTNA010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.370"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..347)
                     /locus_tag="OO161_004275"
     CDS             complement(<1..347)
                     /locus_tag="OO161_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR5465074.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEG"
     gene            complement(368..994)
                     /locus_tag="OO161_004276"
     CDS             complement(368..994)
                     /locus_tag="OO161_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5465075.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(1450..>1609)
                     /gene="fumD"
                     /locus_tag="OO161_004277"
     CDS             complement(1450..>1609)
                     /gene="fumD"
                     /locus_tag="OO161_004277"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5465076.1"
                     /translation="VGKVVLEMRDLGQEPKHIVIAGVLRTALANKRIQRSELDKQAME
                     TVINALVK"
BASE COUNT          458 a          371 c          318 g          462 t
ORIGIN      
        1 ttcccttcga taataatgac gtcgtatcca gcgaatttca tttgcgctgc aaaaaagcca
       61 cccatatggg catcgacaac taaatttcct ttggtaaaag tagaaagtga ggtgatattt
      121 acgcgagaac tacagggggc accagatcca gttaatgggc cggtagcaaa gactaattta
      181 ttcgcttcat cgaaaggttt cgtgcctggc ggtacttcgt catacataat tttgtagccg
      241 aagcccatgc caccgacaaa acttttaaac ttactggaat cttcgagggt aatatttcct
      301 gtcgtgagat tgactcttaa tatattacct gtccaaccgt tagccatgat tttttccttt
      361 gcaagattca cacagtaata tctttccact cgataatttt taacgcccct gttgggcagg
      421 cgtttgcaca ttcaccgcat aacacacatt tcgaggattt tttactttcg gtatttacgg
      481 tggccatcat ccacgggcat gccgtagtac aggcgctaca gccaatacag cgtttatgat
      541 cgacggtaat acagccttct ttctgctgcc aggtaatcgc accaatcggg cagacgttca
      601 tgcattgcgg ctctttgcat tgacggcagg tgtccgcggt atagttgaga tcgccataca
      661 ggccgccgcc agagccaacc ccgttgtcgc caaagaaata attgcgatgg attttgatac
      721 gggagaagaa tgtccctact gagccatcgt tgaagttggt acaagagatt tcacagcggt
      781 ggcaacccgt gcatcgcgct cgctgggtca ccagcacccc tttgggtgta tttatcaacc
      841 ctactgtgcc gctatcaata tcttcctgtt tgcaacccaa aagtgacagt aacgcagggg
      901 caattgttag accagcaagg ccttttcctg atatacgcaa gaattctaat cgcgttaagc
      961 caatatctaa tagtggacga tcaaccgggt tcatttattt cgcatcctct tcgcaaacac
     1021 cgtgctgaca attcagcagc ctgatattaa gcattcaact aatacgctgc tgactctgtc
     1081 cccaacacca gatattttcc ctaacccgat agtgtatgag aaaagattta acgccgataa
     1141 actaatcctt tatggttagg agaaaaaata atgtgattat tctgagccct taacattgat
     1201 cgttatcaat taaaattaca aagcaagaaa ttataagtga actgatattt attatcattt
     1261 gaaataaatt taacttaagc gacaattatt tgtgacgttc attgcagaaa ataaaaataa
     1321 tattatcgtt ataaattaaa atacataccg caattcgtat aatttcagca gttgttattt
     1381 agaatgatta actggaccgc aactgaagca agaatatcag gatgaagctt accgcctcat
     1441 cctgaatggt cattttacca gtgcgttaat gacggtttcc atcgcctgct tatccagctc
     1501 actgcgctga atgcgtttat tggctaatgc tgtacgtaat acgcctgcga taacaatatg
     1561 ctttggttcc tgccccagat cgcgcatttc cagcacaact ttaccgaca
//
LOCUS       DAJTNA010000364         1581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTNA010000364 DAJTNA010000000
VERSION     DAJTNA010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.371"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(9..224)
                     /locus_tag="OO161_004278"
     CDS             complement(9..224)
                     /locus_tag="OO161_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5465077.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(517..591)
                     /locus_tag="OO161_004279"
     tRNA            complement(517..591)
                     /locus_tag="OO161_004279"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(556..558),aa:Gly,seq:tcc)
     gene            complement(595..670)
                     /locus_tag="OO161_004280"
     tRNA            complement(595..670)
                     /locus_tag="OO161_004280"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(635..637),aa:Thr,seq:tgt)
     gene            complement(673..749)
                     /locus_tag="OO161_004281"
     tRNA            complement(673..749)
                     /locus_tag="OO161_004281"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(713..715),aa:Arg,seq:tct)
     gene            complement(751..827)
                     /locus_tag="OO161_004282"
     tRNA            complement(751..827)
                     /locus_tag="OO161_004282"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(791..793),aa:Ile,seq:cat)
     gene            complement(1017..1571)
                     /locus_tag="OO161_004283"
     CDS             complement(1017..1571)
                     /locus_tag="OO161_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5465078.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          426 a          425 c          330 g          400 t
ORIGIN      
        1 tggcgacatt attcacctct cgcagcctta cgcttatctt ctcttatttt gaaatacaga
       61 ttcgtcagat aagtcagaag ccccagaagc agacttccca gcacaccaat cgcagcccac
      121 tgtgatggac tgacctgatc caaccactgc aaaaaccagt agccggcact gccggcggag
      181 gtgccgtagg caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa
      241 ataacggatg gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaaaaacgca
      301 aaagcgggcg aaacgatata tacagtaagg aaagcactct atccaacaaa ccacccacag
      361 ttaatcggaa taaaagcaga gtgcttatga atgatcgcac gcccgaaggt tagtatttct
      421 gcacagcaat tttgcaaaaa aaggcgatca ttcataactt aaacgtcttt cagtcactcc
      481 gggatttccc atcatcgcag actgaaagac tctgactgga gcgggcagcg ggaatcgaac
      541 ccgcatcatc agcttggaag gctgaggtaa tagccattat acgatgcccg catatggtgc
      601 cgactaccgg aatcgaactg gtgacctact gattacaagt cagttgctct gcctgctgag
      661 ctaagtcggc gctggcccac caccgaggac tcgaacctcg caccgtcaac ttagaaggtt
      721 gatgctctat ccggatgagc tagtggtggt tggtggccct tgctggactt gaaccagcga
      781 cctggcgatt atgagtcgct cgctctgacc aactgagcta aagggccgga ggcagaataa
      841 taaccatatg tcatcacatc tgcaaactca tctgaccacc agcacgttta acgtcctgtg
      901 ccgtttttca ggtataaaaa aacccgcata aagcggactc tttcaaatgt ccatgtctgc
      961 tattcgcctc gcggtacagc tttgcgaagt gtacaagaat tgaagcagtt tttacgtcaa
     1021 aaagcaataa cttttttctc tataccaaaa gccataacca ttggtttgta caaaataaat
     1081 tctgccacct ttagccaatg ctcaatgcgt ctttcacagg ttcttaaact ccatttcggg
     1141 tgtgcatcat tcagcagttc agccattttg cgtttggtca tcccccgccc ctcataccgt
     1201 tgccgaagga cgctaatcaa tcctggatgc tctgccagca cctcacttat gacccgatca
     1261 atacataacg cctctgcatc agtacaatgc gccagccagc tcttttgctt accgttaatc
     1321 atatcccgca aaaaagcctc aagttcagac ttgttcagac ctgctttttt catcctccgg
     1381 agcgcctcgt taattgccgt ttttgtcagc tttttagagg ccaacaactg gttgaacata
     1441 ttccccgtct taccgccgcc aatatacgac cagcgccccc acatgcgcag ttttccctga
     1501 atccagacac tttccagcgt ggtgagacga aggtgttccc cgcttttgcc tgtatttgtt
     1561 gggtaaatca taaataacct t
//
LOCUS       DAJTNA010000365         1580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTNA010000365 DAJTNA010000000
VERSION     DAJTNA010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.372"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            97..1580
                     /locus_tag="OO161_004284"
                     /pseudo
     CDS             97..1580
                     /locus_tag="OO161_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
BASE COUNT          551 a          287 c          279 g          463 t
ORIGIN      
        1 gcgatttatg atatttcttt acttgtaatc atattaaatt ttttcactgc tctgatagtt
       61 aaaattcaag acatcaataa acaatgagat atttaaatga ttactcgtat tcctcgtagt
      121 tctttctctg caaatattaa taatacagcc cagacaaatg aacaccaaac cctgagtgaa
      181 ttgttttata aagaactcga ggataaattt tctggcaagg agctggcgac gcctctatta
      241 aaaagcttct cagagaactg tagacaaaat ggtcgtcata tctttagtaa caaggatttt
      301 gtcattaatt ttccaagtcc gtcttacaag ctgataagaa agaaattacg ataattaata
      361 aaaatgaaaa cacgacactc actcaaacca ttgctccaat atttgaagaa tacctaatgg
      421 aaattttacc tcaacgctca gacactcttg ataaacaaga attaaaccta aactcagtta
      481 gaaaagaaaa agaattccca agagttaaac ttaatggtca atgttatttt ccggggcgcc
      541 cccaaaaccg tatagtatgc cgacacattg ctgcacaata tattaatgat atttatcaga
      601 atgttgatta caaaccccat aaagatgatt actcttcagc tgaaaaattt ctcacgcact
      661 tcaacaaaaa atgcaaaaac cagactttgg agttggtttc cagccgtcct gaggggcgtt
      721 gcgttgctgc ctgcggtgat ttcgggctag ttatgaaagc atattttgac aagatggaat
      781 caaatggcat cagtgttatg gcagccatat tactggtgga taaccatgct ttgacggtcc
      841 ggctaagaat aaagaacaca actgaaggat gtacccatta cgtggtttcg gtttatgatc
      901 ctaatgtaac taacgataaa ataagaatta tgagcgaaag caaagaggat attaaacact
      961 attctctgat ggattttatg aatgtagatt atagcctcct gaaatggtca aatgatcata
     1021 ttattaacca atctgttgca ataattccag cacttccgaa agaacagcta ttgatgttaa
     1081 aaggatctgt ggatgaaata acccctccgt tatcacctgc aacgatgaat ttgctaatgg
     1141 caattggtca gaatcaccaa cttacgcaac tgatgattca actccagaaa atgccagaac
     1201 tacatagaac agaaatgttg actgcctata atagtattaa cctgacaggt ttatatttgg
     1261 ctataaatta tggtaatgct gatatcgttg agactatttt caattcattg tcagaaacag
     1321 gatatgaagg gttactctcg aaaaaaaatc tcatgcatat tctggaggca aaagataaaa
     1381 atggtttttc ttgattattt ttagcgatat cacgtaagga taaaaatgtt gtaacctcga
     1441 ttctgaacgc cttacctaaa ctggccgcaa cacatcattt agataacgaa caagtgtata
     1501 aattcctgag tgccaaaaat agaacgtcca gccatgtttt ataccatgtt atggcgaatg
     1561 gtgatgccga catgggtaat
//
LOCUS       DAJTNA010000366         1575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTNA010000366 DAJTNA010000000
VERSION     DAJTNA010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.373"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..184)
                     /locus_tag="OO161_004285"
     CDS             complement(<1..184)
                     /locus_tag="OO161_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465079.1"
                     /translation="MKIEELRENFSENGLYAVRVENGAIVSHCCIRCLQSQQRKSGAV
                     LFYFCNGLLTDDFILRE"
     gene            complement(434..589)
                     /locus_tag="OO161_004286"
     CDS             complement(434..589)
                     /locus_tag="OO161_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5465080.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYKSRK"
     gene            complement(817..1482)
                     /locus_tag="OO161_004287"
     CDS             complement(817..1482)
                     /locus_tag="OO161_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5465081.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          400 a          411 c          349 g          415 t
ORIGIN      
        1 cctcacgcaa aataaaatcg tccgtcagaa gtccattaca aaaataaaat aacacagcac
       61 cgctcttcct ttgttgagat tgcaaacatc taatgcagca atggctgaca atcgctccat
      121 tctcaacgcg cacagcatag aggccatttt cactaaaatt ttcacgcaat tcttcgattt
      181 tcatcttcag aatccttcca gataaatagc tctcccctgt tcggggtcca tccctcttct
      241 ccctgcgcgc tacttaagtg agtcgattct atctgcgaag gtgcgcgaat caaattcacc
      301 ggaaataaac aacaaaaaac ccgccgaaac gctgagagat cacagcgatg ggcgggttaa
      361 gtgcgggtgc gttgaggatg cctgacacat cagaggtggg cggggatggg atcagctccc
      421 cgcccggtca ctcttacttc ctggatttgt agtctacgaa gacagcgacc tccgtctggc
      481 cggttcggat tcgtacctca cagaggtctt tcctcgttac cagtgccgtc actatgacgg
      541 ttaaacagat gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct
      601 ccttgccttt cggcatgtaa gaggctaacc tacatgtgca aagcatgaaa ttggcctcag
      661 attaatgtta agcgtcttgc cggacgcgta atgttaactg gggcttttct ctatctgcct
      721 tttagtgttc atgcctgaag cagatagcct caagcacccg cagtcattct acttacctcg
      781 ccaatatgaa atcaatcaga aaggtgcccc ataaaatcac tccttctctt ctttaccgta
      841 gtggagttga ccaattttga taagagggcg tccctgagat ttgcggtgta gattggtatc
      901 gcgcagagaa tacacacagc cacaatattc ctgctgatag aatttttcgc gcttgctgat
      961 ttcaatcata cgggacgagc ctccctgctt gcgccagtta taatcccagt acaccatgcc
     1021 cggataatgc gcgacggctc gccgcccaca gtcgttaacc tgctgcatat ttttccagcg
     1081 tgaaatgccc agtgaactgc tgatcacact gaaaccattt tcagcggcgt acagcgctgt
     1141 ccgctcaaaa cgcatgtcaa aacacatggt gcaacggatc cctcgttcgg gctcccattc
     1201 cattcctttg gctcgttcaa accagttgtc tgtgtcgtaa tcagcatcaa taaacggcac
     1261 gccgtgttgt tcagcaaagc gaatattctc atccttacga attaaatact ctttctgagg
     1321 atgaatgttc gggttgtaga aaaagatggt gtagtcgatt cccgaggcct gaagcgcctc
     1381 catcacctca ccggaacatg gagcacagca agagtgcagt agtagtttgt ttgccccgtt
     1441 tgggagctcc aatttgggcc gtttgaaatc agcaactgtc ataaatatgt ttattggggt
     1501 cataaaaata tcaaagagtg tagcattaga acggggctat cggaaacaga tgtgtgactg
     1561 ctccccgccc tttcg
//
LOCUS       DAJTNA010000367         1552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTNA010000367 DAJTNA010000000
VERSION     DAJTNA010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.374"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            203..355
                     /gene="hokA"
                     /locus_tag="OO161_004288"
     CDS             203..355
                     /gene="hokA"
                     /locus_tag="OO161_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5465082.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(534..746)
                     /gene="cspA"
                     /locus_tag="OO161_004289"
     CDS             complement(534..746)
                     /gene="cspA"
                     /locus_tag="OO161_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5465083.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1027..1317)
                     /locus_tag="OO161_004290"
     CDS             complement(1027..1317)
                     /locus_tag="OO161_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR5465084.1"
                     /translation="MEYKDPTHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSNQLME"
BASE COUNT          437 a          325 c          332 g          458 t
ORIGIN      
        1 ttttaggatt atatcaagga gaagaaacaa acttattaag ctagaatagc tacgggtgct
       61 tgagactgtt tgtctcaggt attcaccgaa aggcagacag agaaaagccc cacctgacta
      121 taaatcaaag tgaggctacc ctatgcctga acaccataag gttagcctct tactcgttgg
      181 aaatcaacac agggggctgg gaatgccgca aaaatacgga ttactttcat taatagtgat
      241 ttgtttcacg cttttatttt tcacctggat ggtaagagat tcactgtgtg aattgcatat
      301 taaacagggg cgttatgagc tggcggcatt tttagcctgc aatttaaaag agtaagagtc
      361 ttcggcggga aattattccc gcctttctta cggcgttgcg cattctcatt gcacccacat
      421 ttattcttca caaaaataat aatagatttt actacgcgat cgattattta tttcctgaaa
      481 aaaaataaaa aaaatccccg ccaaatggca gattctgtgc ttttaagcag agattacagg
      541 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      601 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      661 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      721 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      781 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      841 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      901 accgttgatg tgcattaagc cacgcattgg cgggtgatgc aacaattatt tttcatattt
      961 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
     1021 aaaagtctat tccatcaact gattacttaa tgccgggttg gcttgaatca aacgcatcaa
     1081 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1141 tgatatgccc aaaacccggg cgaaatcatc gatttttaac cctgtccctt ttcgtaactg
     1201 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1261 aaaaacaatc tgttccaggc tgctcaacag ctcatgcgtt ggatctttat attccattga
     1321 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1381 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1441 agtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttac
     1501 cactcctgtt ttgtaaagat ttcgaaaatc taaagttctg cgaaggcgta aa
//
LOCUS       DAJTNA010000368         1547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTNA010000368 DAJTNA010000000
VERSION     DAJTNA010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.375"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(289..1398)
                     /gene="fepE"
                     /locus_tag="OO161_004291"
     CDS             complement(289..1398)
                     /gene="fepE"
                     /locus_tag="OO161_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5465085.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNLLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          502 a          340 c          250 g          455 t
ORIGIN      
        1 tggcagcggc cactggtaaa caggatgtgc gagagatgta ttgagagttc ttgaagtggc
       61 agccttactg aggctacact agaaggacag atttggtgac atgtctcgca caagactgtt
      121 accacggttc agaagttccc caacttactg aaccttcgaa aaaccacctc cccaggtggt
      181 tttttcgttt tctagagtag aagactacgg ctgtaaggga atgatctcaa gaagatcctt
      241 tgaacttttt ataaaagttg cttataaaat gatgcatcag cacaactttt actcaatcac
      301 actcatcttt ctggcctcca tggcatgacg taaaagtata tagccacatg caagcatccc
      361 tccaatgaca gaagaaagaa ttacaattat ggcattactt ggcccatcct ttctgaccgg
      421 taacgttggg ctcaattgat actgaaacgg cataaaatca acatccccca ctttcgccac
      481 aaccaattga ttcacaacat attgacgatt tcgcaactcc ccactcaatt ccgaaacatc
      541 cttgattgat tttttgatgt tcaatttagt tgctatacca tcagaaccga gagccacagg
      601 aaaatctggg tcatccttca taatctgacc attgctgtat acaggttttt ttattccagc
      661 cgcattagca acctccagtg aataattgag gcgtcgaata tctgcattca gctggtttct
      721 aatttttatc tcatccagtg caagaatttc tttttcaaca ttagttttaa cttctagttt
      781 atttcttata tcttccatca aatcccttac aacaagggaa gaaacataat taatataccc
      841 agacaacaca tcgtgagctt ctttacttgt tggagcagta aaactcagag tccatgatac
      901 atacaatgat gacttgtcgt ttttcttact ggcattatca tcaatcgact tcatattttt
      961 actcaactca acaattgcac gatgcagttc gagagcgcta atgttattct ttttaataat
     1021 ctccataaca taaggagatg atctcatata ctcattcaac aaattaacgg actgaaattt
     1081 cttaataaac agagcaaatg cgctatcacg attgatatca aaatccactc ccagaacatg
     1141 aagcttggaa agctccttat ttaattcact ccattgaaca gactcagcag gagtaaccac
     1201 agcagaacta gtccattttt gtggcaaaac aaaagaaaca agaaagccaa tacaagcaaa
     1261 aaatagaatc accccaacaa tttttatttt tgctgcccat aaaacgctaa tcaagccaaa
     1321 aaggtcaatt tcttcattac cattcggcaa catgttgtaa ttagaaaaat gttcgtcaga
     1381 gacctttgag attttcattg aaaaaaacac aatatattaa catatggttt ttttattcta
     1441 tcatatgtta ggaaaaaacc taaaaaccat tcagcagaca tttaacataa cattttattg
     1501 cgtgcaccgc ctcagaggct ctcagggcat ttctgacggg aaaccgg
//
LOCUS       DAJTNA010000369         1527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTNA010000369 DAJTNA010000000
VERSION     DAJTNA010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.376"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            143..1527
                     /locus_tag="OO161_004292"
                     /pseudo
     CDS             143..1527
                     /locus_tag="OO161_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          513 a          224 c          266 g          524 t
ORIGIN      
        1 tttttatagt acttcacttc ttcttcacta agaatgtcca ttatctcaat gccttatcag
       61 agattctttt cctttcgccg gtagtgtctg gacgctcagg ctacttttcc aggttatttt
      121 atttctgtat tacagaggtt ttatgatagg tcatatcaca aattctggtg gcaataactc
      181 tttgatgaaa catgatgtgg ttcaaggaaa taatatagta gatcttgatt tactacgtaa
      241 tttcaatggg gtgacaggtt taaacagaga taactttatt tatatcagtg atatattttt
      301 aaatataaaa caacggaacg aaaaaatcat gcaataaata tgttacgtga agtctcaatt
      361 agtaatgata ctataagtgt aaaattctac agaaatgaaa aaatagaatg cgcttgtaat
      421 tttctgatgg ataaagatgc gcaggggtat atcgacctgt ctgattttga tgtaacaaat
      481 tgtcatttta aaggtgacgt tatttctaag gtgtcttttt tatcatcaaa tctacaacat
      541 gtaactttcg aatgtaaaga aattgagaat tgcaatttta ctaaggcaac agttgataat
      601 gtcatattta aatgtcgacg tttacacaat gtgatttttc tcaaaacgag tggcgaatgt
      661 gttgatttta gccaaaatat tcttgataca gttgacttct cgcagagtca acttggtcat
      721 agtaattttc gcgaatgtca gattagaaat tcaaacttcg ataattgtta tctttacgct
      781 tcgcacttca ccagagcaga gtttctgtct gccaaagaaa tatcatttat taaatcgaat
      841 ttgacagctg ttatgtttga tcatgtgcga atgtcgacag ggaattttaa agattgcatt
      901 acagaacaat tagaattaac tattgattat tcagatatat ttgggaatga agatctcgat
      961 ggttatatca ataacattat aaaaatgatt gatacattgc cagataatgc aatgatattg
     1021 aaatccgttc tggcagtaaa actggttatg caattaaaaa ttcttaatat tgttaataaa
     1081 aactttattg agaatatgaa gaaaacattt agccattgtc cttatataaa agatccaatt
     1141 atacgcagtt atatccattc tggtgaagat aacaagttcg atgattttat gcgtcaacat
     1201 cgattcagca aggtggattt cgatacccaa cagatgatcc attttattaa caggtttaat
     1261 atgaataaag ggctgattga taaaaataac aattttttat ccaacttatc gatcaggcct
     1321 tacgatcaac ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc
     1381 gtagtgatga tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca
     1441 ctaataaatt aacgcgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc
     1501 ctgttatggt ggtaagctcc cagcgct
//
LOCUS       DAJTNA010000370         1517 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTNA010000370 DAJTNA010000000
VERSION     DAJTNA010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.377"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..1474
                     /gene="wzxC"
                     /locus_tag="OO161_004293"
                     /pseudo
     CDS             1..1474
                     /gene="wzxC"
                     /locus_tag="OO161_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
BASE COUNT          289 a          367 c          459 g          402 t
ORIGIN      
        1 gcttacgtga aaaaaccatc agcggcgcga agtggtcggc gattgccacg gtgatcatca
       61 tcggcctcgg gctggtgcag atgaccgtgc tggcgcagat tatcgacaac caccagttcg
      121 gcctgctgac tgtgtcgctg gtgattatcg cgctggcaga tacgctttct gacttcggta
      181 tcgctaactc gattattcag cgaaaagaaa tcagtcacct tgaactcacc acgttgtact
      241 ggctgaacgt cgggctgggg atcgtggtgt gcgtggcggt gtttttgttg agtgatctca
      301 tcggcgacgt gctgaataac ccggacctgg caccgttgat taaaacattg tcgctggcgt
      361 ttgtggtgat cccccacggg caacagttcc gcgcgttgat gcagaaggag ctggagttca
      421 acaaaatcgg catgatcgaa accagcgcgg tactggcggg cttcactttt acggtggtta
      481 gcgcccattt ctggccgctg gcgatgaccg cgatcctcgg ttatctggtc aatagtgcgg
      541 tgagaacgct gctgtttggc tactttggcc gcaaaattta tcgccccggt ctgcatttct
      601 cgctggcgtc ggtggcaccg aacttacgct ttggtgcctg gctgacggcg gacagcatca
      661 tcaactatct caataccaac ctttcaacgc tcgtgctggc gcgtattctc ggcgcgggcg
      721 tggcaggggg atacaacctg gcgtacaacg tggccgttgt gccaccgatg aagctgaacc
      781 ccatcatcac ccgcgtgttg tttccggcgt ttgccaaaat tcaggacgac accgaaaagc
      841 tgcgcgtgaa cttctacaag ctgctgtcgg tggtggggat tatcaacttt ccggcgctgc
      901 tcgggctgat ggtggtgtca aataactttg taccactggt atttggcgaa aagtggaaca
      961 gcattattcc ggtgctgcaa ttgctatgtg tggtgggtct gctgcgctcc gtaggtaacc
     1021 cgattggttc gctgctgatg gcgaaagcgc gggtcgatat cagctttaaa ttcaacgtat
     1081 tcaaaacatt tctgtttatt ccggcgattg ttattggtgg gcagatggcg ggcgcaatcg
     1141 gcgtcacgct cggtttcctg ctggtgcaga ttatcaacac cattctgagt tacttcgtga
     1201 tgattaaacc ggttcttggt tccagttatc gccagtacat cctgagttta tggctgccgt
     1261 tttatctctc gctgccgacg ctggtggtca gttatgcgct gggcattgtg ctgaaagggc
     1321 aactgggctg gggatgctgc tggcggtgca aatagccgcg ggcgtgctgg cgtttgtggt
     1381 gatgattgtg ctgtcgcgcc atccgctggt ggtggaagtg aagcgtcagt tttgtcgcag
     1441 cgaaaaaatg aaaatgcttt tatgggcggg gtgagttgct gttttcggtt ccgacaacgg
     1501 ttgccggatg cggcgta
//
LOCUS       DAJTNA010000371         1514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTNA010000371 DAJTNA010000000
VERSION     DAJTNA010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.378"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1090..1416
                     /locus_tag="OO161_004294"
     CDS             1090..1416
                     /locus_tag="OO161_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465086.1"
                     /translation="MFKYAEMLMAEEDDKVCELIFKQQDNLILKINDRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLILCALDVNSWRGKSKYPDYYLLRDEMPVRLVSIIFPTLL
                     ALFLLA"
BASE COUNT          453 a          262 c          296 g          503 t
ORIGIN      
        1 ccttattcag tcgaccttca aaataaacgt ttaataggga caatcaccat ttggattgtc
       61 cgctgaggag gcgaacgttt tcctagacag ctttatcatg tcagcccaca ttgttggcgc
      121 tgacaatact aaggacaaac gatggggaag tatcttttta gcgttatttt cgggagtaaa
      181 aaacatggta tctttccgta tgtcagttgt atcttgctgg tattcgtagt cgtgataagg
      241 catacaacga tatttatctt cacgttagtg aagaataagt tgttttgtga tctatgttaa
      301 tctgggatta caatccgggc ttgatacatc gcatcacgac ttttattctc ttcagaatag
      361 aatatgttac tccccgtttt acaggctcag gatttgaatt acaaattata cttttaagtg
      421 aacaataacc aggggtattt cttatttcct gaaaccaggc ttggcccatt tcgggatgag
      481 tttgaaaaaa ctcttgacgc atacgatagt gatgaaataa acgaaacaaa ttacgtcaac
      541 aaactcaggc gtcttgcctg acatgctcct gacatcattg atattcacgc tcatctggca
      601 tatgcatttg cgctctttat gctgcaattc tggcgaatgt tcatttacag cgtcatcagg
      661 atgccgttat actcattgat aaaatactaa catataaccc tgataataat caggggacac
      721 gctggttact ggggacggaa ttgttacgtg ctgcaagcat gacagggctc tgtacgtatt
      781 gacaggacat gtcaatgaat ttccccccta ctggtacgag cttgctcttt tacattttat
      841 gaatgaggca tatattaatg ctgcaacggc atttcgatat agatttttaa caagcatata
      901 ttgttgaaat gctttgttag aatctttatc cattccctgg ggctgtatag tatgattttt
      961 caggtatttc tgaatacagc caaatattat tgcaaaatat tctctaaatt attaattaaa
     1021 tatccataag cattattgcc tgggaactgg acatataatt actcttctgt gctaaaggaa
     1081 cgttctgaaa tgtttaagta tgcagagatg ttgatggcgg aagaagatga taaagtatgt
     1141 gaacttattt ttaaacaaca agataatttg attttaaaaa taaatgatag atttcccaga
     1201 ataattataa agaaataccg aacattacaa ggtaaagtcg tatggctatg gatattgcca
     1261 tttgttattg aatggttaat cttgtgtgct ttagacgtaa atagctggcg aggcaaaagt
     1321 aaataccctg attattattt gttaagagat gagatgcctg tgcgtctagt gtctattata
     1381 ttcccaacat tattggcgct attcttgctg gcgtaggcat cgccatacta tgaatattgt
     1441 gtttgtcgtg attcaatttt tatttataac ctctacccgg agcaaattca ggtaggtcat
     1501 gcataaaaat atct
//
LOCUS       DAJTNA010000372         1497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTNA010000372 DAJTNA010000000
VERSION     DAJTNA010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.379"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..567
                     /gene="envY"
                     /locus_tag="OO161_004295"
     CDS             <1..567
                     /gene="envY"
                     /locus_tag="OO161_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5465087.1"
                     /translation="YLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAANHSKQQP
                     SKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQHYWNLRI
                     VASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQLCGYSST
                     SYFISVFKAFYGLTPLNYLTKQRQKVMW"
     gene            complement(613..689)
                     /locus_tag="OO161_004296"
     tRNA            complement(613..689)
                     /locus_tag="OO161_004296"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(653..655),aa:Arg,seq:tct)
     gene            932..1497
                     /gene="fimZ"
                     /locus_tag="OO161_004297"
                     /pseudo
     CDS             932..1497
                     /gene="fimZ"
                     /locus_tag="OO161_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
BASE COUNT          438 a          329 c          320 g          410 t
ORIGIN      
        1 tatctgttgt ttttaaataa gaacttaacc tgtgtaaagc cctggtcgcg gctggcaacc
       61 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccgc caaccacagc
      121 aagcagcaac ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
      181 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
      241 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
      301 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
      361 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
      421 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
      481 tcgtacttta tctctgtttt taaggcgttt tacggcctga caccgttgaa ttatctcacc
      541 aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
      601 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
      661 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
      721 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
      781 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
      841 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
      901 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
      961 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaacag tgaattgcag
     1021 attgtcctga aaacggatga ttaccgcata accatcgatt atctccgaac ccgtcctgtt
     1081 gatttaatca ttatggatat agacttgccc ggaacagacg gttttacctt cctgaaaagg
     1141 atcaaacaaa tccagagcac agtgaaagtg ttatttttat catcgaaatc agaatgcttt
     1201 tatgctggca gagcgataca agctggtgct aacggttttg tcagtaaatg caatgatcag
     1261 aatgatattt ttcatgccgt tcagatgatc ctctccggat acacgttttt tcccagcgaa
     1321 acgcttaact atataaaaag caataaatgt agtacgaata gttcaacggt cactgtgcta
     1381 tctaatcgtg aagtgaccat attacgttat ctggttagcg gattatctaa taaagaaatt
     1441 gccgataagt tattgcttag caataaaaca gttagtgcgc ataaatctaa tatttat
//
LOCUS       DAJTNA010000373         1487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTNA010000373 DAJTNA010000000
VERSION     DAJTNA010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.380"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            246..>1487
                     /gene="tnaA"
                     /locus_tag="OO161_004298"
     CDS             246..>1487
                     /gene="tnaA"
                     /locus_tag="OO161_004298"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5465088.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDRE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCP"
BASE COUNT          394 a          346 c          382 g          365 t
ORIGIN      
        1 taagattgtc gatcaccacc cttaatttac ccttctgtag ccatcaccag agccaaaccg
       61 attagattca atgtgaacga ttagtttgct atatcttaat tttgcctttt gcaaaggcca
      121 tctctcgttt atttacttat tttagtaaat gatggtgctt gtatatatac atggcgaatt
      181 aatggatatt gcagatgtaa tattcacagg gatcactgta attaaaataa atgaaggatt
      241 atgtaatgga aaactttaaa catctccctg aaccgttccg cattcgtgtt attgagccag
      301 taaaacgtac tactcgcgct tatcgtgaag aagcaattat taaatccggt atgaacccgt
      361 tcctgctgga tcgcgaagat gtgtttatcg atttactgac cgacagcggc accggggcgg
      421 taacccaaag tatgcaggca gcgatgatgc gcggcgacga agcctacagc ggcagccgca
      481 gctactatgc gttagccgag tcagtgaaaa atatctttgg ttatcaatat actattccga
      541 ctcaccaggg ccgtggcgca gagcaaatct atattccggt actgattaaa aaacgcgagc
      601 aggaaaaagg cctggatcgc agcaaaatgg tggcattctc taactatttc tttgatacca
      661 cgcagggcca tagccagatt aacggctgta ccgtgcgtaa cgtctatatc aaagaagcct
      721 tcgatacggg cgtgcgttac gactttaaag gcaactttga cctcgaagga ttagaacgcg
      781 gtattgaaga agttggcccg aataacgtgc cgtatatcgt tgcaaccatc accagtaact
      841 ctgcaggtgg tcagccggtt tcactggcaa acttaaaagc gatgtacagc atcgcgaaga
      901 aatacgatat tccggtggta atggactccg cacgctttgc tgaaaacgcc tatttcatca
      961 agcagcgtga agcagaatac aaagactgga ccatcgagca gatcacccgc gaaacctaca
     1021 aatatgccga tatgctggcg atgtccgcca agaaagatgc gatggtgccg atgggcggct
     1081 tgctgtgcat gaaagacgac agcttctttg atgtgtacac cgagtgcaga accctttgcg
     1141 tggtgcagga aggcttcccg acatatggcg gcctggaagg cggcgcgatg gagcgtctgg
     1201 cggtaggtct gtatgacggc atgaatctcg actggctggc ttatcgtatc gcgcaggtac
     1261 agtatctggt cgatggtctg gaagagattg gcgttgtctg ccagcaggcg ggcggtcacg
     1321 cggcattcgt tgatgccggt aaactgctgc cgcatatccc ggcagaccag ttcccggcac
     1381 aggcgctggc gtgcgagctg tataaagtcg ccggtatccg tgcggtagaa attggctctt
     1441 tcctgttagg ccgcgatccg aaaaccggta aacaactgcc atgcccg
//
LOCUS       DAJTNA010000374         1407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTNA010000374 DAJTNA010000000
VERSION     DAJTNA010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.381"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(66..551)
                     /locus_tag="OO161_004299"
     CDS             complement(66..551)
                     /locus_tag="OO161_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HCR5465089.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGATAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDAADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     I"
     gene            complement(580..806)
                     /locus_tag="OO161_004300"
                     /pseudo
     CDS             complement(580..806)
                     /locus_tag="OO161_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(803..1072)
                     /locus_tag="OO161_004301"
     CDS             complement(803..1072)
                     /locus_tag="OO161_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465090.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
BASE COUNT          374 a          358 c          285 g          390 t
ORIGIN      
        1 ctgcatgaca aggtcatcgg gcattatctg aacataaaac actatcagta agttggaatc
       61 attacctaaa tattgcgaaa atctattggg aataaacttt cactttcttt atcatacagc
      121 atattacctg tattaagatc gtaatggatt atactcaact cattcaattt acatatcaac
      181 tgtagataaa ggctctctat attatcaggg atatcagctg catcaatgtc actaagggga
      241 gtcccaggca cgcgcagcat tcggaggtac acatctccgc catactgtat aacaacggat
      301 gcttcatcgc cataaaaagc attaaaaagc tcagattctt gccaagccat ttccagaatc
      361 tcattgtact ggttgccaat aagatcatac tttttataca aagcagatga atcattcaca
      421 tcttcaaaga tttctgctgt cgccccctga cctattaact taccgagaat gggctccgtt
      481 actatgccag catgagaatt attattctca aatggaaaag gtgtttgaat aatggtagat
      541 gttattttca tgtcatacct ttgttacgat atttaaaaat cacaagagat tttcacacaa
      601 aataacgtta cacgctacct tgtgtggtcc agatgttcct tccacacgac cattgcggcc
      661 tttatacggc tgccctgcca ggctgctggc tgaataacca atccgttcgt ccggttcaat
      721 ggcggccatc aggttctgct ccgcaatgta ctacgaatgc gcttcctgtc ctggatggcc
      781 agcatggtcc agaacatctc catcatttat cagcaatctt cctgctcatt tcatcccgcc
      841 atgcagcagc agaggattcg accatgtcat ggggaagcac atcaccgcat tcgagttgct
      901 gacggcctgc cgcaacctgg tcacggaacc aagaattgtg ctgccgttca acggtctggc
      961 gcataaaatc ccggattaac tgagagccat tacggtccat gctttttgca gcggccataa
     1021 aagcatcctt cagttctgcg tcgattctta cactcatatt aacctgtgcc atttgccacc
     1081 tcgtcattac cacgtatata cacagtatat aactgttcag atatcgtcac caggatatgc
     1141 cgcgccagcg gcatgtaagg cggcactccg ttgttgcata tggcagagga gtaaaaccgc
     1201 cgaagcccgg cgtaagccgg tacgtattga caggtttcac ctgtcctatc actggcgctg
     1261 acagttttat gcgatgtgat tatgaatggt gcagttgtga gccgttttca gacaatcgca
     1321 gggccagtac ctcgtcagtc agctgacggt aaatctgctg ttcagtctca cgcatcacct
     1381 gtgcaccggc ttccctctcc gcatccg
//
LOCUS       DAJTNA010000375         1397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTNA010000375 DAJTNA010000000
VERSION     DAJTNA010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.382"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            7..690
                     /locus_tag="OO161_004302"
     CDS             7..690
                     /locus_tag="OO161_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCR5465091.1"
                     /translation="MKTQHDHYDRLSVRLSVIISRLLAGESLELKALADEFGVSERTL
                     QRDFQQRLIHLDLEHSSGSYRLTCRTGREHSPNTFAFIRNSGISGIIPAQNRRLIRLI
                     TSNSGSSPCLIGHGGLPSPVTQTACFLELAEAIRDYRAVTLLVNGKSYEAVAPRRLIF
                     QSKNWFLVATRTRNLQVFRMEDVNAVTVLDTSFRRKPELDALTASEEFISALPHFRFI
                     SDVIQTFRE"
     gene            729..>1397
                     /locus_tag="OO161_004303"
     CDS             729..>1397
                     /locus_tag="OO161_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR5465092.1"
                     /translation="MNDPVKGEGRMITDASGVTRREGGILGMWSNIFPERNGSNEQKG
                     DSKPHIPDIRDILDIFPQPLREKLLNKISEAIDYEPVIGVMGKTGAGKSSVCNALFKG
                     EVCAVSDVEACTREVQELRIRFGKHSLKIIDIPGVGENARRDKEYEYLYRNLLPSLDL
                     ILWVIKGDDRAFSADEHFYNNVLLPAGGGERVLFVLNQVDKIEPFREWDTHLHQPSPA
                     QRINI"
BASE COUNT          364 a          332 c          346 g          355 t
ORIGIN      
        1 cccactatga aaacacagca tgatcattat gaccgcctgt ccgtcaggct atcggtcatt
       61 atcagccgct tactggctgg cgaatctctg gagcttaaag cgttagctga tgaatttggt
      121 gtctcagaac gcaccctaca gagggatttt cagcaacgcc tgatccatct ggatctcgag
      181 cattccagcg gaagctaccg acttacctgc cgaacgggcc gggagcactc acccaacaca
      241 tttgcgttca tccgtaattc cgggatttca gggattattc cggctcagaa tcgcaggtta
      301 attcgactga ttaccagtaa ctccggaagc tcaccgtgcc ttatagggca tggagggctg
      361 ccatctccag ttacacaaac cgcctgtttt ctggaactgg cggaagctat ccgtgactac
      421 cgcgccgtta cattattagt taacggtaaa agctacgaag cagtagctcc ccgccgtctg
      481 atatttcagt ccaaaaactg gtttcttgtt gctacccgca ccagaaattt acaggtgttt
      541 cggatggaag atgttaacgc cgtaaccgtt ctggatacct cttttcgcag aaagcctgaa
      601 cttgatgctc tgactgccag tgaagagttt atttcagccc tccctcactt tcgtttcata
      661 agtgacgtaa ttcagacctt cagagagtaa cagacaacgt tcatttcaaa ttaaaaagga
      721 gacctcgtat gaatgacccg gtaaagggcg aaggccggat gattactgat gccagtggcg
      781 tcaccagaag ggaaggcggg attttgggaa tgtggagcaa tatatttcca gaacgtaatg
      841 gtagcaacga gcaaaaaggt gatagcaagc cgcacatacc cgacatcagg gatattcttg
      901 atattttccc tcagccactg cgggaaaagc tgttgaacaa aatcagtgaa gccatcgact
      961 atgaacccgt gattggtgtc atgggaaaaa ctggcgcggg gaaaagcagc gtatgtaatg
     1021 cgctgtttaa aggggaggtt tgtgcagtta gcgacgttga ggcatgtact cgcgaagttc
     1081 aggaattgcg tatacgcttc ggcaaacatt cgctaaagat cattgatata cctggtgtcg
     1141 gtgagaatgc ccggcgggac aaggagtatg aatatttata ccgtaatttg cttccctccc
     1201 tagatcttat cctgtgggta ataaaaggag acgatcgggc cttttctgcg gatgagcatt
     1261 tctacaataa tgttctgctc cctgctggtg ggggtgagcg cgttttattc gtcctcaacc
     1321 aggttgataa aattgagcca ttccgcgagt gggatactca tctgcatcag ccatctcctg
     1381 cacagcgcat caacatc
//
LOCUS       DAJTNA010000376         1394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTNA010000376 DAJTNA010000000
VERSION     DAJTNA010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.383"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..550)
                     /locus_tag="OO161_004304"
     CDS             complement(<1..550)
                     /locus_tag="OO161_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5465093.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYPGEDAAEQLELMRSVFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLL"
     gene            complement(599..946)
                     /gene="tnpB"
                     /locus_tag="OO161_004305"
     CDS             complement(599..946)
                     /gene="tnpB"
                     /locus_tag="OO161_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5465094.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDAPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(943..1332)
                     /locus_tag="OO161_004306"
     CDS             complement(943..1332)
                     /locus_tag="OO161_004306"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5465095.1"
                     /translation="MSDMQKNVNPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALE
                     NGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEV
                     TFRHGTLRFNGNVSEKLLTLLIQELKR"
BASE COUNT          285 a          384 c          395 g          330 t
ORIGIN      
        1 ccagcagccc cggtcctgcg ataccccgct cgatgggccg tgaaggcgcg ggggcctgca
       61 cgatggcatc gcactgagta caggcatgct tttcacgtac agtccggata acccggaaga
      121 cgctgcgcat caactccagc tgttcggcgg catcctcacc cggatagctc agtgagcctc
      181 cacattccgg gcagcatgac gctgccggca gcagccgttt ttcatcgcgg gggagtgatt
      241 cggggaacgg tttgcgggtg cgggtttgac gcagcgggcg ctgcacggcc gggtcgtcaa
      301 cccgaccggt aagggtatca ctttctttct gaagtgcctt caggtcagct tccatctgtg
      361 cgatacgacg ggagactttt tcggagcggc tgccgaagtt catccggcgc agcttatcca
      421 gctgtgcctg cagatggtct atttcgcgtt cacgctcgtt cagcttttcc agcagggcac
      481 ggttcagcgc ctcctgttcg gcaaggagac gtttcagtgc attgatatcg tcaggaagtg
      541 agctgctcat accgggtata ttaccaggct cattcagcgt cgaccaggat aaagaggctt
      601 acaacatagt cagggacgta agcagtcttt taggctgccg ccagtcgata ccttcaagga
      661 gcatcgccag ctgtgccggt gtgaggaaca ctttgccatc ccgggctgac ggccaggcga
      721 agcggccgcg ctccagccgt ttggtcagca gacacagtcc atcgccggta gaccagagga
      781 gctttacctg actgccatta cgcccacgga agataaaaac gtgacctgac atcggagcgt
      841 ctttcagcgt cgtctgcacc tttgccgcca ggccgttgaa gccatttctc atatcggtga
      901 taccggcaac cagccaaatt ttggtcccgg aaggtaacgg gatcatcgct tcagttcctg
      961 tatcagcaga gtcaggagct tttcgctgac attgccattg aagcggagcg tcccgtgccg
     1021 gaacgttacc tcacagctga tactgagggt ttccggatcc tctgcgagcg attctggctg
     1081 ttcggcagct gcatcgagag tcacaggaag tagctggggg ctctctgaag aaggtaatag
     1141 cagctttccc tcgcgccatt gttgtcgcca tttgaacaac agattggcgt taatgccatt
     1201 ttcaagagca agttttgaga tggatatccc gggttcacag gaggcagcaa cgagctgctg
     1261 tttaaattcg ggaggataat tagggcagcc ttttcgcctg ccgggattca catttttctg
     1321 catatctgac actttggttc ccactactta tttggtggac accactttgt ctaattcgtc
     1381 agattctgac caga
//
LOCUS       DAJTNA010000377         1390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTNA010000377 DAJTNA010000000
VERSION     DAJTNA010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.384"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(493..759)
                     /locus_tag="OO161_004307"
     CDS             complement(493..759)
                     /locus_tag="OO161_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465096.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEVGIVEKQSQWHSNI"
     gene            complement(941..1339)
                     /locus_tag="OO161_004308"
                     /pseudo
     CDS             complement(941..1339)
                     /locus_tag="OO161_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          360 a          297 c          292 g          441 t
ORIGIN      
        1 cccgcaaaat gagtcaggga aaacgacaca atcaggcgct tatcgccctg gcgagacgac
       61 gctgcgacgt tctgttcgcc atgatgcgcg acgggacttt ttataccccg caggggtcat
      121 aacatgcttg acaacttaat agggcccccc cccaaagccg tctatgggaa ggagtctgct
      181 gttattccag agaatggtaa ttcagtcata tatttctcca tctatgactt gtcaatacca
      241 tcctgatatt tgtgggtcag caatatattc tactaaaagg tatttaaggt cattgcgtcc
      301 ctgcactcga ataatggtga agaaagcatg atgaatatat cacgataacg ttcagtatgt
      361 atcgatgata gatggagtta ctcctctctg aaatagaatt actgctctac tttttctgtt
      421 tccagaaatt attagtaaca aaccctgata tattcttttg aaaatggagc ttaaacgaat
      481 ctgaaattaa cttcagatat tagaatgcca ttggctttgt ttttcaacaa ttcctacttc
      541 atttatcttt gtcaaccatt gcatttctgt agcagtgaat ttcaacgttt tcgtccggtt
      601 ggcagcaggt atttgtaatt cgtatatggc tgctttttta ttgaaaacag tatatccatc
      661 tgtagtaaca gtagaagata ataatacagg atttgacact tcaaggatga tattttcttg
      721 tttttgtgga aggcaaggga atatagtttg tgtaagcata ttgcttcctt atgttatatt
      781 ttagtaaaga atttattttt tacattatag tcggctaaat gaagtttact gtaaaaaaac
      841 gatacttttt tcgtaagcga tgagatgtgg agccagaaaa atggctgcgt tacgtcattg
      901 aactgtcctt gacccacgat attcagacag tcgttgtatc ttaagttgac attacccgct
      961 gacgtcgata ttcccttggt gagagataac ccaacgcact atgcggatgg ttttcattgt
     1021 aatgctcgat cgccactgca aggttatgca atgccgttct tggtttcggc atgaacgcga
     1081 tgcagtcttc cttcatcgtt ttcacgaacc gttctgctat tccattactc tgagggctac
     1141 tgactgccgt cgtgcagggc tccagattca actctctggc gaactgccgt gtttcatgag
     1201 ccgtataagc cgaaccgtta tcggccactg gattggccgt tccggtcctt tatcgccata
     1261 tcgctgacat gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt
     1321 ctatggcttc acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg
     1381 gcattcggtt
//
LOCUS       DAJTNA010000378         1387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTNA010000378 DAJTNA010000000
VERSION     DAJTNA010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.385"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..888)
                     /locus_tag="OO161_004309"
     CDS             complement(<1..888)
                     /locus_tag="OO161_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5465097.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGG"
     gene            complement(1069..1387)
                     /locus_tag="OO161_004310"
                     /pseudo
     CDS             complement(1069..1387)
                     /locus_tag="OO161_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT          380 a          318 c          325 g          364 t
ORIGIN      
        1 gccgcccggt aagtcatgga cgtaactgac atactggaac agattatctt cgctctggtt
       61 gatgataatg tcgcgccccg aatcggtatc ggttagcgtg acattgagaa aacgccagag
      121 gagagggcgg tcatgagtgt aaaagatatt ccgcaaattg tttttgttaa tatccagcag
      181 cacaatccct tttagctgcc ctgcaacata caccggcgtc agcaaactca tcacgttctg
      241 tttagtacgc tgatcctggt aaatgctcga caatacggta cgcccggtaa ataccttttc
      301 aatatctttg cgatcgatac caatacttcc tgactgcagg aaggaccagt tattcatcgc
      361 aaagtattca acattaaccg gctcataaaa atagacatag cggtcattga gatcgagata
      421 atagtgaaat ttctcttcgg ccatcccaag acctgaaccg taatcatcct ggtggcggtt
      481 gccttccact gcctgatcaa aagcgggtaa tagtgctgca gcttccacaa ttgtgtcgca
      541 gtcagtggat gcggtttgta gtgtgccgcg taagcccggg tatttatgag cagtcaggtt
      601 taagccatgg gtattattgg cagactcaaa agcctcgcag atggcgcgcc gctgttctgg
      661 cgacacttcc gactgctcag cagcgagagc gcgcatcaca tgcgcggcaa tactctgatt
      721 ctgatactta tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc
      781 actcattgct ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat
      841 gacacttgcg gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac
      901 tccttcggtt agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc
      961 gctaattata ctcatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa
     1021 ttgtatgcaa aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatatgatg
     1081 aataatcact ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt
     1141 catgacaagc gagatattac gcgtgtcacc cgttgaaaaa accgtgccaa tactgatggt
     1201 catttgttca ggaacatcat atctgttact aaagccggtc aggagttcga catttttatg
     1261 tatacgatcc gcaatgattt ttgcctaggc actattgagt tcggtaaata gcaagccaaa
     1321 cacctcaccc tccagtcgcg ctaaaatatc atcagggcgg atgctttgct ggataatatc
     1381 gacaatt
//
LOCUS       DAJTNA010000379         1387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTNA010000379 DAJTNA010000000
VERSION     DAJTNA010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.386"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            470..1258
                     /gene="virF"
                     /locus_tag="OO161_004311"
     CDS             470..1258
                     /gene="virF"
                     /locus_tag="OO161_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5465098.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTINEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1200..>1387)
                     /locus_tag="OO161_004312"
     CDS             complement(1200..>1387)
                     /locus_tag="OO161_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5465099.1"
                     /translation="APQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAMGYEAFK
                     IFYDISKISLELYHNIH"
BASE COUNT          470 a          236 c          240 g          441 t
ORIGIN      
        1 gtagcaacag ctcacccatg cggctctgac aacgatgtta acccgcgtta aatatcccac
       61 gttggccagc atctgtctca cggaaagtca tactgcctta ccaatatcaa caccaaccgg
      121 atttgtggag cctccagtct gaaggggctt tatgcgttcc gtataggata ttatgatact
      181 ggagttttgc gaagctccac tcctttccga acatttacac agatattgcc taagaaaagt
      241 gaaaccataa tatggttata gtccctttca gtgcaaatac ttagcttgtt gcacagagaa
      301 atagaagctg cataagctct ttcttcaaaa aatgtaaata aagttaaata taggaaaaat
      361 tacttaatct atcttaataa cggaaagatt ttgtatacaa tcactgttac agaaatttct
      421 tagttactct gtaaacacta aatatagttt ggtatattct gttgaattta tgatggatat
      481 gggacataaa aacaaaatag atataaaggt tcgcttgcat aactatatta ttttatatgc
      541 aaaaaggtgt tcaatgacgg ttagctcagg caatgaaact ttgactatca atgaagggca
      601 aattgctttt atagagcgaa atatacaaat aaacgtctcc ataaaaaaat ctgatagcat
      661 taatccattt gagattataa gccttgacag aaatttatta ttaagcatta ttagaataat
      721 ggaaccaatt tattcatttc aacactccta ttctgaggag aaaagggggt taaacaaaaa
      781 aatattcctc ctctctgagg aggaggtttc tatcgatttg ttcaaatcta taaaagagat
      841 gcctttcggc aaaagaaaga tctatagttt agcttgcctt ttatcagctg tttctgatga
      901 ggaagcttta tatacttcga tatcgatagc ttcttctctt agtttttctg atcagataag
      961 gaagattgtt gaaaaaaaca tcgagaagag atggcgtctt tctgatattt caaataactt
     1021 gaatttatca gaaatagctg ttagaaaacg attggagagt gaaaaattaa catttcaaca
     1081 aatccttctt gatattcgca tgcatcatgc agcaaagctt ttattgaata gtcaaagcta
     1141 tattaatgat gtatcaagac ttatcggaat atcaagccca tcttatttta taaggaaatt
     1201 taatgaatat tatggtataa ctccaaagaa attttactta tatcataaaa aattttaaat
     1261 gcttcatagc ccatcgctat tgccagatgg gttttcccca cacctgatgg ccctagtaat
     1321 acgatatttt cattacgttc tatgaagctg agtgagcgta acgactggag ttgcttctgc
     1381 ggtgctc
//
LOCUS       DAJTNA010000380         1384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTNA010000380 DAJTNA010000000
VERSION     DAJTNA010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.387"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>1384
                     /locus_tag="OO161_004313"
     CDS             <1..>1384
                     /locus_tag="OO161_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5465100.1"
                     /translation="VDQFDTEAFRTDKLELTSGNIADHNGNVISGVFDINSSDYVLNA
                     DLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGTEQDNSSVDNVVAATGNYK
                     VRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKADLGAYTYQAEQRGNTVVLQQ
                     MELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDN
                     GTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYS
                     SAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAG
                     YVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYF
                     KLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVD
                     QDWSANVGNAA"
BASE COUNT          355 a          342 c          349 g          338 t
ORIGIN      
        1 atgttgatca gttcgatact gaagctttcc gtactgataa actggaactg accagcggca
       61 acattgctga ccataatggc aacgtaattt ctggtgtgtt cgacatcaac agcagcgatt
      121 acgttctgaa cgctgatctg gtgaacgacc gtacgtggga tacttccaag tctaactacg
      181 gttacggtat tgttgctatg aactctgatg gtcacctgac tatcaatggt aacggcgacg
      241 tagacaacgg tactgaacag gataacagct ctgttgataa cgttgttgct gcaaccggta
      301 actacaaagt tcgtatcgac aacgcaactg gcgctggcac tatcgctgat tacaaagata
      361 aagaacttat tcgggtaaac gacgctaaca ccaacgcgac cttctctgct gctaacaaag
      421 ctgacctggg tgcatacacc tatcaggctg aacagcgcgg taacaccgtt gttctgcaac
      481 agatggagct gaccgactac gctaacatgg cgctgagcat cccgtctgcg aacaccaata
      541 tctggaacct ggaacaagac accgttggta ctcgtctgac caactctcgt catggcctgg
      601 ctgataacgg cggcgcatgg gtaagctact tcggtggtaa cttcaacggc gacaacggca
      661 ccatcaacta tgatcaggat gttaacggca tcatggtcgg tgttgatacc aaaattgacg
      721 gtaacaacgc taagtggatc gtcggtgcgg ctgcaggctt cgctaaaggt gacatgaatg
      781 accgttctgg tcaggtggat caagacagcc agactgccta catctactct tctgctcact
      841 tcgcgaacaa cgtctttgtt gatggtagct tgagctactc tcacttcaac aacgacctgt
      901 ctgcaaccat gagcaacggt acttacgttg acggtagcac caactctgac gcgtggggct
      961 tcggtttgaa agccggttac gacttcaaac tgggtgatgc tggttacgtg actccttacg
     1021 gcagcgtttc tggtctgttc cagtctggtg atgactacca gctgagcaac gacatgaaag
     1081 ttgacggtca gtcttacgac agcatgcgtt atgaactggg tgtagatgca ggttatacct
     1141 tcacctacag cgaagatcag gctctgactc cgtacttcaa actggcttac gtctacgacg
     1201 actcaaacaa cgataacgat gtgaacggtg attccatcga taacggtact gaagggtctg
     1261 cggtacgtgt tggtctgggt actcagttca gcttcaccaa gaacttcagc gcctataccg
     1321 atgctaacta cctcggtggt ggtgacgtag atcaagactg gtccgcgaac gtgggtaatg
     1381 ctgc
//
LOCUS       DAJTNA010000381         1371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTNA010000381 DAJTNA010000000
VERSION     DAJTNA010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.388"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..1325)
                     /gene="ag43"
                     /locus_tag="OO161_004314"
     CDS             complement(<1..1325)
                     /gene="ag43"
                     /locus_tag="OO161_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5465101.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGT"
BASE COUNT          267 a          438 c          330 g          336 t
ORIGIN      
        1 gttccacgat gtccgacagt ggtgttgctg gccagtcctc cggcattaac cgtaaagctg
       61 ccgccatttt ccagcagcag tccgctggcc tgaccggatg tgccatcaat actgaactta
      121 ccgctggcat tggtcccctc aacagtggca ccactgtctg caatcagggc accaccggat
      181 gtcatggtga cacctgttgc cttaccaccg gcagacactg ccagggttcc gccgtcatcc
      241 acccgtgttt tctgcgctga atggccttcc agtacatcca ggcgaccgcc ggactccaga
      301 acaactccgt cagccttacc gttttccacc gtgaaattgc ccagacggtt gctgccaaga
      361 accgttgccg cagtactggt aaccagtgct ccaccctgtt tcagggtgac atgtgtggcg
      421 gtaccaccgg cgttcacctg cagtttgcct ttctggttaa cggtggtgaa atccgccaga
      481 ccaccttcct tgataatctg ccagccgtca ctgtttacaa cagtgccaga cgccgtaccg
      541 ccgttgtgca catactggta accgccattc agtgtggtat ccagcgcatg cccgtgtacc
      601 gtctggtcgc cgccggcata aaccacagtg gtatttgctg ttccttcagc agccacaatc
      661 tgacgaccat tttcattgat agtggtacgt acggcatttc cgcgaacaaa ctgcccggta
      721 tcaccatttt ctgcatctgg tcccccttcc gcgccggtat tcacaaccgt gtcggttgcc
      781 attgcgccgg atttgacggt ctgccagccc ttctcattaa tgacggtacc cgttgcaatc
      841 ccgccttcat gtacccactg ctcaccgccg ttcagagtgg tattcactgc ctgcccctga
      901 aggctctgtc cgcctccggc actgataacc gtgtctgaaa cgcttcctcc ggcattcact
      961 ctctgaagac caccaccggt gacagtagtg ttgttggcga taccgccatt ttgtatccat
     1021 tgcccgccgg tattggcctc gttatccggc ccatattcca gaccgctact gatggtcatt
     1081 ccgttggccg taccgagaac aatctggttg tcatgatttg tcagtgttcc gccgttcacg
     1141 gtttctcccg cctgtacaat cgtgtcagca gccagtgccg ggactgatgt gacagcagca
     1201 agagacagca caaccgccac accggcgcgt tttccccgtg agcgggccag ttcggaagcc
     1261 accaccaggg tgcccgtaat gtgattccat accagcctgt agctggtgtt cagatgtcgt
     1321 ttcatcagct tttccttaca gagggtgaat aaaaaagccg gtacccacaa a
//
LOCUS       DAJTNA010000382         1331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTNA010000382 DAJTNA010000000
VERSION     DAJTNA010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.389"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..1256
                     /locus_tag="OO161_004315"
     CDS             <1..1256
                     /locus_tag="OO161_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5465102.1"
                     /translation="VAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAAFV
                     TTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTPRAGGIVLPIINSVAV
                     ALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISWGG
                     WALATGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLGVF
                     VLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGIIGL
                     SSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAMLPV
                     FAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGAVL
                     TILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT          282 a          316 c          349 g          384 t
ORIGIN      
        1 ccgttgctgc ctcaatggtg gtggtcggta acttatccga cggtgcgttt aagaccaccg
       61 ccgtattaag cggttactct tcaggtacta cctggctggt gttctcggcg tttaccttaa
      121 gcgccgcatt tgtgaccacc ggtttaggta aacgtattgc ctatctgctg attggtaaaa
      181 tcggtaacac cacgctgggt ctgggttacg ttacggtatt cctcgatctg gtactggctc
      241 cggcaacacc gtctaacacc ccgcgtgcgg gcggcattgt gttaccgatc atcaacagcg
      301 tggcggtggc tttggggtcc gaaccggaaa aaagtccgcg tcgtgtcgga cattacctga
      361 tgatgtccat ttacatggtc accaaaacta ccagctatat gttctttacc gcaatggcgg
      421 ggaacattct ggcgctgaaa atgatcaacg acattctgca cctgcaaatt agctggggtg
      481 gatgggcgct ggccaccgga ttgccgggca tcattatgct gctggtcacc ccgctggtaa
      541 tttacaccat gtatccacca gaaattaaaa aggtggataa caaaaccatc gctaaagcgg
      601 gccttgccga actgggaccg atgaaaatcc gcgaaaaaat gctgctcggt gtctttgtgc
      661 tggcgctact gggctggatt ttcagtaagt ctctgggggt tgatgaatcc actgtggcaa
      721 tcgttgttat ggcgaccatg ctgctgctgg gtatcgttac ctgggaagac gtggttaaaa
      781 ataaaggcgg ctggaatacc ttaatctggt acggcggtat tatcggatta agctccttat
      841 tatcgaaagt taaattcttc gaatggttag ctgaagtctt taaaaataac ctggcatttg
      901 atggtcacgg taacgttgct ttcttcgtta ttattttcct cagcattatc gtgcgttatt
      961 tcttcgcttc cggtagtgcc tatatcgttg ctatgttacc ggtatttgcc atgctggcga
     1021 acgtctccgg cgcaccgtta atgttaaccg cgctggcact gttgttctcc aactcctatg
     1081 gcggcatggt tactcactat ggcggcgcgg caggtccggt catctttggc gtgggttata
     1141 acgatattaa atcctggtgg ttggtcggtg cggtactgac gatattaacc ttcctggtgc
     1201 atatcaccct cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg ctgtaaatat
     1261 tctcgtcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact
     1321 tatgtaagct c
//
LOCUS       DAJTNA010000383         1313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTNA010000383 DAJTNA010000000
VERSION     DAJTNA010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.390"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>1313
                     /locus_tag="OO161_004316"
     CDS             <1..>1313
                     /locus_tag="OO161_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5465103.1"
                     /translation="AGNPVIGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSG
                     TLTLMPQLNGVDAAKAPAVVNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDEND
                     KPVKEQKQQLNTAVSIDNVKPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWN
                     EDLHTAGFIIDANPQSAKIATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTF
                     AVLSGSATSFNNQNTAKTDVNGLAAIDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDS
                     STAQVDLQKSKNEVVADGNDSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVN
                     SHDGIATATLTSLKNGDYTVTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTN
                     TAPQHMTATLQDKNGNPLKDKEITFSVPNDVASRFSISNGGKGMT"
BASE COUNT          395 a          320 c          316 g          282 t
ORIGIN      
        1 cagcaggtaa tcctgttatc gggctggtgc tctcgacgcg tcacgaaggt gttcaggaca
       61 tcaccctttc tgactggaaa gataatggtg acggaagcta tacccagatc ctgaccacag
      121 gtgcgatgtc tggcacgctg acgcttatgc cacagctgaa cggtgtggat gcggctaaag
      181 cccccgccgt ggtgaatatc atttctgttt cgtcatcccg gactcactcg tcaattaaga
      241 ttgataagga ccgttatctc tccggcaatc ctatcgaggt aacggtagaa ctgagagatg
      301 aaaatgacaa acctgttaag gagcaaaaac agcaactgaa taccgcagtc agcatcgaca
      361 atgtgaaacc tggtgtcact acagactgga aagaaaccgc agatggcgtc tataaggcaa
      421 cctataccgc ctataccaaa ggcagtgggc ttactgcgaa gctattaatg caaaactgga
      481 atgaagattt gcataccgct ggatttatca tcgacgccaa cccgcagtca gcgaaaattg
      541 cgacattatc tgccagcaat aatggtgtgc tcgccaatga gaatgcagca aacaccgtct
      601 cggtcaatgt cgctgatgaa ggaagcaacc caatcaatga tcataccgtc acgtttgcgg
      661 tattaagcgg atcggcaact tcctttaaca atcaaaacac cgcaaaaacg gatgttaatg
      721 gtctggcggc tattgatctg aaaagtagta agcaggaaga caacacggtt gaagtcaccc
      781 ttgaaaatgg cgtgaaacaa acgttaatcg tcagttttgt cggcgactca agtaccgcac
      841 aggttgatct gcagaagtcg aaaaatgaag tggtcgctga cggcaatgac agcgccacaa
      901 tgaccgcgac agttcgggat gcaaaaggca acctgctcaa tgacgtcaag gtcaccttca
      961 atgttaattc agcagcagcg aaactgagcc aaaccgaagt gaatagccac gacgggatcg
     1021 ccacagctac gctgaccagt ttgaaaaatg gtgattatac ggttacggcc tctgtgagct
     1081 ctggttctca ggctaatcaa caggtgaatt ttatcggtga tcaaagtact gcagccctga
     1141 ccctcagtgt gccttcaggt gatatcaccg tcaccaacac agctccgcaa catatgactg
     1201 caaccttgca ggataaaaat ggcaacccac taaaagataa agaaatcacc ttctctgtgc
     1261 caaacgacgt cgcaagtcgg ttctcgatta gcaacggcgg aaaaggcatg acg
//
LOCUS       DAJTNA010000384         1308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTNA010000384 DAJTNA010000000
VERSION     DAJTNA010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.391"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..166)
                     /locus_tag="OO161_004317"
     CDS             complement(<1..166)
                     /locus_tag="OO161_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465104.1"
                     /translation="MSGNNNTITAADAIITLTVNNLYPSGVRLQGFATDNVYGTDPLV
                     LAETARGIDGK"
     gene            complement(167..>1308)
                     /locus_tag="OO161_004318"
     CDS             complement(167..>1308)
                     /locus_tag="OO161_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HCR5465105.1"
                     /translation="MLSINGKSVSIEVNFNGVASFAAAATALQTALTAAVATVVFDTT
                     QNAFVITVAGAKPGSTTITFGSGSAAELLKMTSNTGAVISRGAPVSDVPDTMAAIKAA
                     SQQWAGFSTVSEVTDEQHLAFSAWANGQGKRYFYVAWTTSGNARVKGSTEHIAYQIIA
                     VNNYSSVVPVFATDGNRAAAVLGYAACLDFIRPEGRVSFKFREYEGLAADVTSGSDYD
                     ALIAAGYNFYGKYAENSVVEDYWADGTITGDFKWLDSFCGQIWLNASLQGAVIALFKS
                     NQTVPYNNEGRALVAASMSDVIQQYKRWGGIREGVTLTEAQKKQINNVVEADVSSTLF
                     ATGYYLYIGDMLPSLRATRSSPSCTLWYCDGGSIQKLVIASTEVQ"
BASE COUNT          304 a          376 c          312 g          316 t
ORIGIN      
        1 gtttaccgtc aataccgcgg gcagtttccg ccagtaccag tggatcggtg ccataaacgt
       61 tatctgttgc aaatccctga agtcgtacgc cggagggata cagattattc actgtcagcg
      121 tgataatggc atccgccgca gtgatggtgt tgttgttacc cgacatttac tggacctccg
      181 tggatgcaat aacaagtttc tggatactac cgccgtcaca gtaccagagc gtacaggacg
      241 gactgctacg ggttgcccgc aaagagggaa gcatatcgcc gatatacagg taatagccgg
      301 tggcaaacaa cgttgaagaa acatctgcct ccacaacatt gttaatctgt ttcttctgcg
      361 cctccgtcag tgtcacccct tcacggatcc ctccccagcg tttgtactgc tggataacgt
      421 cactcattga cgctgcaacc agcgcccgcc cttcattgtt gtaggggacc gtctggtttg
      481 acttgaataa tgcgatcacc gccccctgca aactggcatt cagccagatt tgcccgcaga
      541 aactgtccag ccatttaaaa tcaccggtaa tagtgccatc cgcccagtaa tcttccacca
      601 cactgttttc cgcatatttt ccgtagaaat tgtaacctgc ggcgatcagc gcatcataat
      661 cgctgccact ggtaacatca gcggccagcc cctcatactc gcggaacttg aatgatacac
      721 gcccctctgg tcggataaaa tcaaggcacg ctgcataccc cagtaccgct gcagcccggt
      781 taccatcagt cgcgaaaaca ggtacaacag agctgtagtt attgacagca attatctggt
      841 aagcgatgtg ttcagtactg ccttttactc tggcgttgcc gctggttgtc catgccacat
      901 aaaagtaacg cttgccctgc ccgtttgccc atgcagaaaa cgccaggtgt tgctcgtcag
      961 tgacctcaga taccgtggaa aaacccgccc attgctggga agcggcctta atggctgcca
     1021 ttgtgtcagg gacatcagat acaggcgcgc cccgggatat caccgcaccg gtgttactgg
     1081 tcatcttcag gagttctgct gccgatccac tgccgaacgt tatcgtggta ctcccgggtt
     1141 tcgccccggc gacagtaatg acaaaagcat tctgtgtggt atcgaatacc actgtagcca
     1201 ctgctgcggt cagcgctgtc tgtagtgccg ttgcagcagc ggcgaagctg gcgacaccgt
     1261 taaaattcac ttcaatgctg acgcttttcc cgttaatact cagcatca
//
LOCUS       DAJTNA010000385         1283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTNA010000385 DAJTNA010000000
VERSION     DAJTNA010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.392"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..213
                     /locus_tag="OO161_004319"
     CDS             <1..213
                     /locus_tag="OO161_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5465106.1"
                     /translation="IAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSA
                     LGYRSPREYLRQRASNGLSDNRCLEI"
     gene            complement(216..302)
                     /locus_tag="OO161_004320"
                     /pseudo
     CDS             complement(216..302)
                     /locus_tag="OO161_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(420..920)
                     /locus_tag="OO161_004321"
     CDS             complement(420..920)
                     /locus_tag="OO161_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5465107.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLMADFTGELHRLIS
                     EYLQCKRRKDKSVEPAPLYYLWSREAVANDTTPYLMVLLLNNDVWCKSREVVALRNLI
                     EQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNAEPGVLEQTAQAYIESHGAIERH
                     LGCSDF"
     gene            complement(1014..>1283)
                     /locus_tag="OO161_004322"
     CDS             complement(1014..>1283)
                     /locus_tag="OO161_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5465108.1"
                     /translation="NKDAYYHLGDYERSDTLRGLITGAWYSALGLELEDHQGLVHFPA
                     NGRYILDINDPDFPEQYQRLLARLDYLTKLDTKASGQRNFGYSGF"
BASE COUNT          371 a          304 c          286 g          322 t
ORIGIN      
        1 atagcagaga gcttcgtgaa aacgataaag cgtgattaca tcagtatcat gcccaaacca
       61 gacgggttaa cggcagcaaa gaaccttgca gaggcgttcg agcattataa cgaatggcat
      121 ccgcatagtg cactgggtta tcgctcgcca cgggaatatc tacggcagcg ggccagtaat
      181 gggttaagtg ataacaggtg tctggaaata taggggcaaa tccaatggcg cagatacgct
      241 tgcggatcga taccattcag acagcaggtc cgttcactac cgtaatcgct gccgaagaac
      301 acattgattg cccctcaata atcctgccgt caacgtcaaa attgctggtc gggctcgact
      361 tatttaattg tgtaaacagg gcctctcagc gaaaggccct tatgcatcac ctgacgtcgt
      421 taaaagtcac tacacccaag atgacgttca atggcaccat gcgattcaat gtaagcctgg
      481 gccgtctgtt ccagtacacc aggctcagcg ttgtatgtgt tagctgcatc aaataccaac
      541 gacagcactt caggatacac aaccagatgt gtaatggagt tatcttcacc caatactttt
      601 ccccacgcct gctcaatcag atttctgaga gccaccacct cacgactctt acaccagaca
      661 tcgttattaa gtagcagtac cataagataa ggagtggtat cgttagccac agcttcccta
      721 ctccagagat aatataaagg ggcgggctca acagatttat ctttacgtcg cttgcactgc
      781 aaatattcag aaattagtct atgcagttca ccagtaaaat cagccatcag agagggaatt
      841 gccttgttca tacctgggca aggtattaat ttaaattgta ataatttaat tttaggatgt
      901 gtagctgcag cccgatacag agttgcaagg acatactttt gccagagggc gttacaggaa
      961 agcttaacgt ttgattctgt atacataata aatcacctta cagttacaat aggtcaaaaa
     1021 ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt cagataatcc
     1081 agacgtgcca ataagcgctg atactgctca gggaaatcag gatcgttaat atccaggatg
     1141 taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc caaccctaaa
     1201 gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc atagtctccc
     1261 aggtggtaat aggcgtcttt gtt
//
LOCUS       DAJTNA010000386         1280 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTNA010000386 DAJTNA010000000
VERSION     DAJTNA010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.393"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            82..1275
                     /locus_tag="OO161_004323"
     CDS             82..1275
                     /locus_tag="OO161_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5465109.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATVALINGASLTLTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          380 a          269 c          295 g          336 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacagacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac agtggcgtta
      181 atcaacggag catctctgac gctgaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagattga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta
//
LOCUS       DAJTNA010000387         1270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTNA010000387 DAJTNA010000000
VERSION     DAJTNA010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.394"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..185)
                     /locus_tag="OO161_004324"
                     /pseudo
     CDS             complement(1..185)
                     /locus_tag="OO161_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(321..569)
                     /locus_tag="OO161_004325"
     CDS             complement(321..569)
                     /locus_tag="OO161_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5465110.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(924..>1270)
                     /locus_tag="OO161_004326"
     CDS             complement(924..>1270)
                     /locus_tag="OO161_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5465111.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          335 a          285 c          298 g          352 t
ORIGIN      
        1 ggcaagagcg ccacatcggg gatttttctg attggtgccg cgcatacagc gatgaaaagg
       61 agatatttgc tcagtgagac tgaccgaaac ctttccgcca gcatcgtcag cttctgccca
      121 gtaaaaagag acgcggaaaa tgcacaacag gcaccacacg tcatgcatgg attcagattg
      181 ctcataattc accagtacag ctataaatcg taaagagaac agcggtacat cgtatgtaag
      241 aacgtaccgc ggctggctgg tgaacttccg atagtgcgag tattgaatga tttccatccg
      301 ttacagattt tacgtgctaa ttagtgaaca aaccactcgt cagcagactc ccaggtatct
      361 ttcagagttt cctgaacaaa agttttagct gaatctttat cggcggtgcg cgtaacagaa
      421 aggccatcgt tgctggtagc ttttacgatc acctctacat cgtcataacg tttactgatg
      481 cgtcgggtta attcttcctt taacgcatcc acagcaccgg ttggcatttt agtcattttt
      541 tctttggcta tgcagatttc aatacgcata aaagtccctc tacactgtgt ttgtatacag
      601 tatcaatttt aactgtatgg ataaacagta tcaagggtga aggaaagtga ttttttgggg
      661 catgcatggg gcaaaaatat ttgttgtggg gcattttggg gcatgattgg gacactttgt
      721 tacatatgaa ctttgccgat ttttatatga catggaaata caacgctttg attcttctaa
      781 ataaaaaaca tgctcttggg cgttctttag tgatttttaa aaattccgta tcacgcagtt
      841 aaagtggcgg gcatactctt caaggctggt gatccccaac cgcacccatt taggatgcga
      901 ccattgcggt agaccgatgt aaatcataac gcgtgaagga tctcttccac gctacgcaca
      961 cgggcgatgc gcgggtagat atgattaatg ctgttattgt gttgctcggc gctggcggca
     1021 ctacaggcgt cttcggcaat caccagatta aagccaagtt cccaggcatt gcgggcggtg
     1081 gattcaacac cgatattggt cgagatccca cataacacta ttgtatcgat acctcggcgg
     1141 cgcagttgca actccagatc cgtaccgtaa aacgcacccc actgacgttt gatgatttcg
     1201 atatcactgt cagttgcacc taatgcagct ggatgttgcc accagttttc gggcaacact
     1261 ttagcggggg
//
LOCUS       DAJTNA010000388         1263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTNA010000388 DAJTNA010000000
VERSION     DAJTNA010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.395"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..1263)
                     /locus_tag="OO161_004327"
                     /pseudo
     CDS             complement(1..1263)
                     /locus_tag="OO161_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          372 a          354 c          280 g          257 t
ORIGIN      
        1 ttgccaacca gtaaaacgct tccgttttca acgcacccca cagcatcggc cagaaaatga
       61 gtcctgcaat tgcccctgtg cgcataatag cgcgggcaaa tccgacccca gtagggcgaa
      121 tagcgggaag ataagagagt gtggcaatgg tcatgcccag cccaccagga cccgctgaat
      181 ggaagaaaat aattgatccc aataacccta aagacagcca gggattagtc gttgccagtg
      241 cgcctaacga cagcaatgct aaggtcacaa cagcaaatcc atacatcgac tgtcggcgag
      301 taccagtcgt tggagaataa acgagccgat catcccgcct gtcaccccac aaagggcatt
      361 cacgacggca gaaccggtca acgcgccagt aagcccgcca cttattattc ccgccaacgt
      421 aagaggaaga taaacaccta cggcgttata ttgccacgcc tgcatggttg ccacgacaca
      481 gccaagaatg gtgcgtttgc ggtaacgatc attaaataag atgccatatc caccactgta
      541 tttattctct gctttttccg agaatttcgc ttctgttgta cccgatgccg caacgcgagc
      601 ctgaacacca taacgttttc ccagaatgtc gcacgcttcc tggtaacgcc cgacgcgtgc
      661 ggcccacatt gcggattcac caataaagaa gtaacgcaaa atcatgaaga tgactgcaaa
      721 aatagctccg atgaatattc cgtaacgcca caattgttct tcagcgatac ccgacagaag
      781 cagcggcaaa agtaccaaat aaacaacggt tgtcgaaaca taccaggcca tttgccagag
      841 gttgaccgac gttccggttt tcttcgacgt tgaaccacga agctctgcca cagcattggt
      901 tgccaaaggg aagtcgatgc ctaaaccaaa gcccatgatg acgcggcaca ccagcaccca
      961 ccagatactg ggagcaatag ctaccgctgc agcaccgatg gtgcacagcc ccatgccaat
     1021 aataaatgcc tgcttttgcc caaagcggtt tattactcgg ttcgctaata aaccgccaat
     1081 taacgcggcg attaatactg atgcattgac ggttgcagca agacccggcg aaatgccgaa
     1141 ttgagcggca atgtatttat taccaaaccc caccatcgcc gcctagtagg catcaatcaa
     1201 tatgccgccc aaagcaataa aaataacgcc gatactggtg ttgattgcag agttcttatt
     1261 aac
//
LOCUS       DAJTNA010000389         1262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTNA010000389 DAJTNA010000000
VERSION     DAJTNA010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.396"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..437
                     /locus_tag="OO161_004328"
                     /pseudo
     CDS             1..437
                     /locus_tag="OO161_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
BASE COUNT          380 a          270 c          251 g          361 t
ORIGIN      
        1 ttcctaaagg tagtagtact tatctgctga tgcgagaaac acattacaat gcgggtatat
       61 tcgtttgggt gttaatgttt tcacgcctga ttataaaaca ccgttatagt gatccttcta
      121 ttgtgccacc gccacctgcc tggcaaatga aagcggcttc gctaatgcac atcatgcttt
      181 atataacctt ccttgcactt cctctgctgg ggattgcttt gatggcttac agtggaaaat
      241 cgtggagttt ccttggtttc aatgtgtctc cctttgttac cccaaacagc gaaattaaag
      301 cactgatata aaatattcac gaaacctggg caaatatagg ttacttttta atcgcagctc
      361 acgctggcgc agctctcttt catcactaca ttcagaaaga taatactctg ttacgaatga
      421 tgcctcgccg caaataagtc gatcccctga caaggaaatg tgaaccatgg acatcaacgc
      481 gtcataaatg tttacgcaag tgcacaatgc accctttcca ctatcaacaa ggaggagcag
      541 aaactgtgta aatcgtacaa tggtaagact tatcaaaaat acaggttaat tttggtattt
      601 aaaccttttt tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca
      661 actcttggaa attatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt
      721 gactcaatta ttgacccctt ctctacgcaa cttcagttcc caccaccaac tttgcggcag
      781 tttcatagga tgaatgtcta aaagaataat ggtgagcgat aagaaaacga ctgaataact
      841 gcacattttc gctcgaaagc ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac
      901 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
      961 ttaggaaaag taaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     1021 ggatttgccc ccccggtaga gttgccccta ctccggtttt cgagaccgat gatacagagt
     1081 cgaaaaatca aagagttaaa aatttttctt ggaaaaaatg tcttaaaatc gtacacgaaa
     1141 aatcaatgaa ttacataaca ttaaagaaga atattccaag ccttcttgag tccaccacct
     1201 gttttcaggt gtcattcaga cgtgatttgc cccagattgg gaaagcacgc ttttttctca
     1261 gc
//
LOCUS       DAJTNA010000390         1252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTNA010000390 DAJTNA010000000
VERSION     DAJTNA010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.397"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>1252
                     /locus_tag="OO161_004329"
     CDS             <1..>1252
                     /locus_tag="OO161_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5465112.1"
                     /translation="CAFAFLSLLFWAIIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVKGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDLTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQG"
BASE COUNT          357 a          261 c          272 g          362 t
ORIGIN      
        1 tgtgcctttg cttttttatc ccttcttttt tgggcaataa taataaaata tgatgtttcc
       61 ttgccgacaa aaaaagtgac aggaatatta ttgctgattg tcatttcggg cagtttattt
      121 tccgcttgtc aatttgctta taaagatgca aaaaataaaa aaacgttcag tccatatata
      181 ctagcgtcgc gatttgctac ctatacgccg tttttcaatc tcaactattt tgctttagca
      241 gcgaaagagc atcaaagatt actctcaatt gcaaacacgg tgccgtattt tcaattatca
      301 gtcagggata caggtattga tacctacgtg ttgattgtgg gggagtctgt acgtgtcgac
      361 aatatgtctt tgtatggata tacacgctct acgacaccgc aagttgaagc acaaagaaaa
      421 cagatcaaac tgtttaatca agcaataagc ggcgcacctt acactgcgct gtcggttccc
      481 ctttctttaa ctgctgattc tgttttgagt catgacattc ataattaccc cgacaacatt
      541 attaatatgg ctaatcaagc aggatttcag actttctggc taagctcgca atccgctttt
      601 cggcagaatg gtacagcagt taccagtatc gccatgcgcg ccatggaaac agtttatgtc
      661 aaaggatttg atgaattgtt gttgccgcat ttatcgcaag cattacagca aaatacgcag
      721 caaaagaaac tgattgttct tcatttaaat ggaagccatg aaccggcttg tagcgcctat
      781 ccgcaatcca gcgccgtgtt tcaaccgcag gacgatcagg atgcctgcta tgacaactcc
      841 attcattaca cagatagttt gctaggtcag gtttttgaat tattaaaaga tcgccgcgcc
      901 tcggtcatgt attttgccga ccacggcctg gaacgtgacc ttacgaagaa gaacgtctat
      961 tttcatggag gcagggaggc tagccagcag gcatatcatg tcccgatgtt tatctggtat
     1021 agccccgttc ttggggatgg cgtggatcgc acaacggaaa acaacatctt ttcgacagct
     1081 tacaataatt accttattaa tgcgtggatg ggggtaacaa agccggaaca gccgcaaacg
     1141 cttgaggaag tgattgcaca ctataaagga gactcacggg ttgtggatgc aaaccatgat
     1201 gttttcgatt atgtgatgtt cagaaaggag tttacagagg ataagcaagg ta
//
LOCUS       DAJTNA010000391         1243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTNA010000391 DAJTNA010000000
VERSION     DAJTNA010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.398"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..1160
                     /locus_tag="OO161_004330"
     CDS             <1..1160
                     /locus_tag="OO161_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5465113.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT          374 a          299 c          252 g          318 t
ORIGIN      
        1 aattacactt aaaacgactc aacagcttaa cgttggcttg ccacgcatta cttgactgta
       61 aaactctcac tcttaccgaa cttggccgta acctgccaac caaagcgaga acaaaacata
      121 acatcaaacg aatcgaccga ttgttaggta atcgtcacct ccacaaagag cgactcgctg
      181 tataccgttg gcatgctagc tttatctgtt cgggcaatac gatgcccatt gtacttgttg
      241 actggtctga tattcgtgag caaaaacgac ttatggtatt gcgagcttca gtcgcactac
      301 acggtcgttc tgttactctt tatgagaaag cgttcccgct ttcagagcaa tgttcaaaga
      361 aagctcatga ccaatttcta gccgaccttg cgagcattct accgagtaac accacaccgc
      421 tcattgtcag tgatgctggc tttaaagtgc catggtataa atccgttgag aagctgggtt
      481 ggtactggtt aagtcgagta agaggaaaag tacaatatgc agacctagga gcggaaaact
      541 ggaaacctat cagcaactta catgatatgt catctagtca ctcaaagact ttaggctata
      601 agaggctgac taaaagcaat ccaatctcat gccaaattct attgtataaa tctcgctcta
      661 aaggccgaaa aaatcagcgc tcgacacgga ctcattgtca ccacccgtca cctaaaatct
      721 actcagcgtc ggcaaaggag ccatgggttc tagcaactaa cttacctgtt gaaattcgaa
      781 cacccaaaca acttgttaat atctattcga agcgaatgca gattgaagaa accttccgag
      841 acttgaaaag tcctgcctac ggactaggcc tacgccatag ccgaacgagc agctcagagc
      901 gttttgatat catgctgcta atcgccctga tgcttcaact aacatgttgg cttgcgggcg
      961 ttcatgctca gaaacaaggt tgggacaagc acttccaggc taacacagtc agaaatcgaa
     1021 acgtactctc aacagttcgc ttaggcatgg aagttttgcg gcattctggc tacacaataa
     1081 caagggaaga cttactcgtg gctgcaaccc tactagctca aaatttattc acacatggtt
     1141 acgctttggg gaaattatga ggggatctct cagtgcattg cctccaattc ccataattta
     1201 ttacgccgat aataacttgg tgtaacctta aaaatgtact taa
//
LOCUS       DAJTNA010000392         1237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTNA010000392 DAJTNA010000000
VERSION     DAJTNA010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.399"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..91
                     /locus_tag="OO161_004331"
     CDS             <1..91
                     /locus_tag="OO161_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5465114.1"
                     /translation="GIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            94..168
                     /gene="yoaJ"
                     /locus_tag="OO161_004332"
     CDS             94..168
                     /gene="yoaJ"
                     /locus_tag="OO161_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5465115.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(227..475)
                     /locus_tag="OO161_004333"
     CDS             complement(227..475)
                     /locus_tag="OO161_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5465116.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(623..805)
                     /locus_tag="OO161_004334"
     CDS             complement(623..805)
                     /locus_tag="OO161_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR5465117.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(809..1169)
                     /gene="yeaR"
                     /locus_tag="OO161_004335"
                     /pseudo
     CDS             complement(809..1169)
                     /gene="yeaR"
                     /locus_tag="OO161_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
BASE COUNT          325 a          300 c          275 g          337 t
ORIGIN      
        1 cggtattatt tttccggttg taatctttat tacagcggtc gtatttttag catggttttt
       61 tattggcggc tatgctgccc cgggggcata aagatgaaaa aaacaacgat tattatgatg
      121 ggtgtggcga ttattgtcgt actcggcact gagctgggat ggtggtaacg tcacctctaa
      181 aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt taagcgttaa cttttaatct
      241 tcctgtagat aaatagcacg acaatcgcac caataacggc aaccacgaag ctgccaaaat
      301 tgaagccatc gactttacca aagccaaaca gcgtgctgat ccatccgccg actacggcac
      361 cgactatccc cagcaggata gtcataaaga atccaccacc atctttacct ggcatgatcc
      421 acttcgccag aataccggca ataagcccaa aaataatcca tgacagaatg cccattgttt
      481 cctcacttat ctgttttgca ttagcgggtt agtcgctgat aaaaagcata gcacaacatc
      541 gggagggcaa gatttgtgac gagcatcacg gaggattttt ttgcgatggc gcagaaattg
      601 cgccatcaac gatcagtgat aattaccaac cacaaacatc atgttcgttt tccgtgtcat
      661 aagaacgcac ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca
      721 aagtcatcgg cgtctctgtt tcataatcga cagaaacgcc attactgtta ttggtgacgg
      781 tcacggtata cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt
      841 gcgcaccttc catcaggact tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt
      901 cagtcatggc ttcaatgttg tgccactttt ctggagggaa caccgcaaac tgccccgctt
      961 cgataaggat cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga
     1021 ccgccccatg cataacggaa aggcgtgggt aacccccctg gcgcgttcct ttatcaagat
     1081 gacgttcgaa ttattccggc aggcgcagtt tgtttattcc agaaaggcgt tgagcgcgta
     1141 tgaatataat tctgtgggat ttgcagcatc cttttccctc cttcggtgaa tgcgctgaaa
     1201 acgtgaggtg tactggcaat agcggacact accattt
//
LOCUS       DAJTNA010000393         1231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTNA010000393 DAJTNA010000000
VERSION     DAJTNA010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.400"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(82..1056)
                     /gene="cysB"
                     /locus_tag="OO161_004336"
     CDS             complement(82..1056)
                     /gene="cysB"
                     /locus_tag="OO161_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5465118.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFFRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          345 a          281 c          284 g          321 t
ORIGIN      
        1 cacggtatag aaataagtaa caaaagagaa tatattccgg caccttcgct acataaaagg
       61 tgccgaaaat aacgcaagaa attatttttc cggcagtttt atatctttaa acatgacctc
      121 aatttcttca ttagagcgca atgcgacagc cgcatcaacg acatcacgcg ttaaatgcgg
      181 tgcaaaacgc tgaatgaaat catacatata actgcgaaaa aaagtactac ggcgaaaacc
      241 aattttggtt gtactgtggc tgaagatatc gtgagcatca acgcgcacaa ggtccggatc
      301 ggcgaccgga tccaccgcca tgctggcaat gacccctacc cccagcccta accggacgta
      361 agttttaatg acgtcagcat ccgttgccgt gaaaacgata cgcggcgtta accctgcgcg
      421 gttaaaggca gtatccagtt ctgaacgtcc ggtaaagccg aaggtatatg tcaccaacgg
      481 atattgcgcc agttcttcaa tggtaatggc ttttttgcct gccagcgggt gatccggagt
      541 gactacaata gcccgattcc agtggtagca cggtaacatc actaaatctt catacagatg
      601 cagcgcttct gtggcgatag cgaaatcggc attgccttta gagacggcat cagcaatttg
      661 tgtcggcgag ccctggtgca tatgcaaaga aacgcgagga taacgctcaa taaagccttt
      721 gatgacgttt ggtaatgcgt agcgtgcctg ggtatgcgtg gtggcgatat acagtgagcc
      781 tttatccggc caggtgtgct ctccggcaac cgattttatg gcatcgactt tcgacaggac
      841 ttcgcgagcg atacgaatta tttcttgccc tgctggcgtt acctgcgtca ggtgcttgcc
      901 gcttcgggaa aaaatttgaa tgcctagctc gtcttccagc attctgactt gtttactgat
      961 cccgggttgt gatgtgtaaa gtccttccgc tgttgatgag acattcaggt tatgattgac
     1021 cacctcaaca atatagcgaa gttgttgtaa tttcatgtta aaccatccac ttagacgcca
     1081 tcagaacatc gttttttcgt cacacctggc gggtgactaa tacgatttaa taataaaaaa
     1141 ggtgctaact ataaccacta tatcatttat agccttagtg tataggtgtg aataaaaaat
     1201 aatatgcaat aaaaaagggc tgctttcgcg a
//
LOCUS       DAJTNA010000394         1228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTNA010000394 DAJTNA010000000
VERSION     DAJTNA010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.401"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..452)
                     /locus_tag="OO161_004337"
     CDS             complement(<1..452)
                     /locus_tag="OO161_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5465119.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDA"
     gene            complement(403..1228)
                     /locus_tag="OO161_004338"
                     /pseudo
     CDS             complement(403..1228)
                     /locus_tag="OO161_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          259 a          343 c          314 g          312 t
ORIGIN      
        1 gcatcactcc acaccatatg tgtttgtgcc gcgacagatt ttgccagcat gaccaccggg
       61 gacaggcggg ccactgacga ggcgtcagcg ttccacgtgc gaccgttcca ttcaaacgtg
      121 aacggcttcg cctcctgttc tgtgcgccat gcttcaattt cctgacgtct ggcctctctg
      181 gccgcttcca gcatttctgg tgtcacagtg aatggggcta tctcacccca tttgccgctt
      241 tgcagttcct gccagatttg ctgacccgtc ggagcgacat catcagcggt ggctgtgtag
      301 gggactgcct ggtccctgtc gtcaaaaaaa acgtcacagt ctactgcgcc actttcggta
      361 taacggggat taatgatttt tttaatttcc acggtgcatt cctcacgatg tgcgaataaa
      421 aagcccgggc attgcgccag agacatgtgc atccggcacc ccggacaggg cgcaatatga
      481 ccccggtaat gaatgctctg aacatcccgt aatgaaaaat tgtggggatg ctatatacgt
      541 tccggtggga gtactgggca ctgaaatccc caccggtccc agtcgtgagc ctctgtatga
      601 ctgccccctg acaagtctga tgactttatc accgtcagct tctccctggt acgcagcaat
      661 aatcagcccg ccaatgtcag ggtctcccca tctgttgcgg acagagctcg ccacgattct
      721 gtaaataata tcttctgtgg ttatatttat tttcacccag ccagtcagtc tggatatggg
      781 ccagtagcag tagcgggtgt gataaatggg gccgttaatg ccgtaaaaag taagggattt
      841 ggctctgtac cgcggttctg ttgtctcagg gcgtgcatca gtccaccgga tgctgagcat
      901 ccccttcaaa ccgtgtgtcg ggtatgatga tgtcgtaggg gccagcaacg gaatattcac
      961 ctggcagcgc attccttacc caggccagga aatcactctt agtgtcaaaa cggataacat
     1021 cttcaggcag aaaagcacac ccaaagccga atgcgccggg tatcgccaga cggccttttg
     1081 tccggtcgta aatgtcgctc tgtgcttcca tcgtggccgc acttttcagc cccagattat
     1141 cccgggactt ctgttgtgcc ttttcgcctg ctgctgcgat ttctgacaga tggttagcca
     1201 ttttcagagt gccggtcagc gcagcatc
//
LOCUS       DAJTNA010000395         1202 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTNA010000395 DAJTNA010000000
VERSION     DAJTNA010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.402"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..416)
                     /locus_tag="OO161_004339"
     CDS             complement(<1..416)
                     /locus_tag="OO161_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465120.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACATGIYCRAVCTKSVYSYQPDLFEDCGGA"
     gene            complement(413..796)
                     /locus_tag="OO161_004340"
     CDS             complement(413..796)
                     /locus_tag="OO161_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465121.1"
                     /translation="MYAKSFLALDGNGRLIGARTAQTAPYAHYTCHLCGSALRYHPQY
                     DTERLWFEHTDDGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGVAE"
     gene            complement(963..>1202)
                     /locus_tag="OO161_004341"
     CDS             complement(963..>1202)
                     /locus_tag="OO161_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5465122.1"
                     /translation="DYRAPGSLAGMIKQAWCSALGVDTGDHTTLAHFPVRPAVWLERD
                     DDTGFQQVLERADYLAKESTKAYGTGERNFGCSRG"
BASE COUNT          272 a          354 c          307 g          269 t
ORIGIN      
        1 gcacctccgc agtcctcaaa caagtctggc tggtagctgt aaacagactt cgtacatacc
       61 gctctgcaat aaatccccgt agcacaggcc tcacagaact tcttcccatg aaaaaactgc
      121 tcacacatca cacaatgcca ctccagcacg accggtaatg gtgataaaaa catcgccata
      181 tcctcaatgt aagggtggga cttttccgga ttcagcacca cacagtccgc cttctgttgc
      241 tcgctcaggg catgtaaatc gtgctcaaac catgccccct gagcatctgt ctgcagaatt
      301 aaccggccat gacaggaaag gcagaaacaa tgccggatat ttctgctgag cgcagagtcg
      361 gcactgataa tgtgctcacc cggcgtgatc cccagccccg tttttatacc gttcattctg
      421 ctaccccctc ctcactgaag cgcccggtct ggcagtgtgt gcagtaccgc tccccataat
      481 aatcgtggtg acattgtctg cagtgccagc tggctttacg caccacgggt aaggcatccg
      541 ggacgaattg ctgcagacgt ttaatcaacc gtatttctct gcgttccggc ctgacataag
      601 gacattgctg cgcgtgctcc gtcagcccgt cgtcagtgtg ttcaaaccag agccgttcgg
      661 tgtcgtattg cgggtggtat ctgagtgcac tgccgcaaag gtggcaggtg tagtgggcat
      721 aaggagcagt ctgtgcggta cgggcaccga tcagacgtcc gttgccatca agtgcgagaa
      781 aagattttgc gtacatagta tatgttcctc tgcgccagac gacacgcagg caacagccgt
      841 cccggtactg gcagcgtggt caggtgtgaa tggcggtttt cagttcagtc tcagaaggga
      901 aaatcagtgt ggtcgctgtg aacacgtcat cacactaagg tcagataagt cgtctgtcgg
      961 gctcagccac gactgcagcc aaagttgcgc tcaccggtgc cgtaagcttt ggtactctcc
     1021 ttcgccagat agtcagcacg ctccagcacc tgctgaaagc cggtgtcatc atcgcgttcc
     1081 agccacaccg ccggcctgac cggaaaatgc gccagcgtgg tatgatctcc ggtatccact
     1141 cccagggcac tgcaccacgc ctgtttaatc atcccggcca gtgatcccgg agcgcggtaa
     1201 tc
//
LOCUS       DAJTNA010000396         1194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTNA010000396 DAJTNA010000000
VERSION     DAJTNA010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.403"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..750)
                     /gene="ipaH4.5"
                     /locus_tag="OO161_004342"
     CDS             complement(<1..750)
                     /gene="ipaH4.5"
                     /locus_tag="OO161_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5465123.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTN"
BASE COUNT          338 a          213 c          259 g          384 t
ORIGIN      
        1 attcgtacaa atgggcaatg tgattatatt ttcgggtaat gaagttagta ggttattgga
       61 aacatccagt gtcaatagtt cttgcggtaa tctggataat ctgcgtagtt gattttcacc
      121 taccttcaaa tattttaagc gctgaggaaa ttctggtagt tcctgtagtc tgttgccgga
      181 aacaaagagt tcttgtagtc tatgaggcaa ttcaggcaag acagtaaggc gattaccttc
      241 aatccgtaat attgttagcg ttgatggaag ctctggtaac gtctccagac tattaaaacg
      301 tgccgagagt gattgtaaat atggtggtaa tgatggcaga catgacaaat ttcgattgaa
      361 tgaaatatta agcttaatca gttgagatgg aagtgtcggg agtatattca agttgttacc
      421 atttacatga agttctgtca aaagcggaga attttcaggt agggagatta actcattgtt
      481 ggaaatatta agttcacgta tatgcaaagg gagtggtggt aaagaacgta gtttcagtaa
      541 gcttaaattg agaacaggct cccgggtatc cagacatatt ttcaataatc gaaccgcctg
      601 gatgcgattt tctgttgttg ttccttccct agcccagtca tcccagatgc ggtggtaatc
      661 tctggtacac tgttcttcta ccgataaacg agatatacat gataagccac aaagggaacg
      721 aaaaaaagaa tgattgttga tcggtttcat ttattaaccc ctcactattc atatcttggt
      781 ctattaatgc aaataaaacg agaaagtctt taaaaaaata atcctagagc tttatcggcg
      841 ttttcattcc agctggagga atatgttcct gtctttccag taaccgtcga ttgttatatc
      901 agtctaccca tgttaatgtg accagttcta ctttcatcca gtttggattg atctttatcc
      961 cctatatttt caaacaattt ttatcattta actcattact gtctcgatat tcccatagca
     1021 aacgatctgg ttggttagcc cctcaaaaaa gtcagttgca cgccgtaaat ccctgccggt
     1081 attgagcgag gatttacggg ttgttgttaa ggtccgtaat cgggtcacag tttttccgga
     1141 gtcaatccgg tctgcggttt atgcttatgc gacgtgatta tgaatggtgc agtc
//
LOCUS       DAJTNA010000397         1160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTNA010000397 DAJTNA010000000
VERSION     DAJTNA010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.404"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(80..289)
                     /locus_tag="OO161_004343"
     CDS             complement(80..289)
                     /locus_tag="OO161_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCR5465124.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(385..759)
                     /locus_tag="OO161_004344"
     CDS             complement(385..759)
                     /locus_tag="OO161_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HCR5465125.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLRRQALELIADAESARKK"
     gene            complement(774..>1160)
                     /locus_tag="OO161_004345"
     CDS             complement(774..>1160)
                     /locus_tag="OO161_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HCR5465126.1"
                     /translation="FLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLD
                     KTLTVNTGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTC
                     VVTDSAEKAQSVTSVECTVTVSAAAG"
BASE COUNT          241 a          396 c          264 g          259 t
ORIGIN      
        1 attttgataa tcagttcacg taacgtcgcc attcaccttt ctccgggcgt aaaaaaaccg
       61 cctcagcggt tctcatcatt catgactgtg ctgcaaagct cagcgcgtct tccagcgccg
      121 caaacggatc cacctccggc ttatcctcat cctcgcccca gcagagcatg acgtccttca
      181 gtgcaacatt catcccctgt gccccgaaaa ccgctttcac gatctgtgca ttacggatat
      241 ccccgcgctc atcacccagc ggggatatcc tgtcgaactc catccacatc atcgcctcgc
      301 tcgcactcag gctgtggcgc agttcggata aggtgcgccc cagacggagc gcaagtcgca
      361 tcagaaagcg aatttccggg cgggctactt ttttctggcc gactctgcat cagcgatcag
      421 ttccagtgcc tgacgccgca accgggcatg taccggacca tagacggcca gcacctgctc
      481 acggtcgtcc ggagtgaaca cccgctgcag gtccgtatca cacaggacat cgcagaacag
      541 cgtcacatcc gcttccaggt tacggcgggt tttcgccacc accgacaggg tatcgtcatc
      601 ctctccatca ccattgagca cttcctgcca cagataccag gcctctgccg aaggctcccg
      661 cagcaccacg ctgacattcc cccattccgg caccttcacc gttttatgac gaaaccctga
      721 cagtctcgcc agcgccagcg ttttcagatc ctttttcatg atgacccatc cccttatccg
      781 gcagctgcgc tcactgtcac ggtgcattca acagacgtca cactctgtgc tttctctgcc
      841 gaatcggtca ccacgcaggt atatttcccc gcatcagcgg actgcgcacc tggcttactg
      901 aaggtgtctg tcgtctgccc gtcaaccggc tgaccatcct tcttccaggc gtatttatac
      961 ggcggcattc ccccgttggc actgactgac attgtcagca gcgcaccggt attcacggta
     1021 agtgtcttat ccagattttt cacaaacgcc agcggtacca caaaggacac cggtttgcct
     1081 ttcagacgca gtgagaacgt tgcagccacc acaccgttgg taccggatga ccaggtgtgc
     1141 tgacgcactt ccgccaggaa
//
LOCUS       DAJTNA010000398         1152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTNA010000398 DAJTNA010000000
VERSION     DAJTNA010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.405"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            263..>1152
                     /locus_tag="OO161_004346"
     CDS             263..>1152
                     /locus_tag="OO161_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCR5465127.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNVLKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAESLIAKIQSETDIPIVIVHGPKGEDNARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMA"
BASE COUNT          370 a          211 c          247 g          324 t
ORIGIN      
        1 cattgccgtc gatctataaa cgaaccttat gagactacct cgacagagcg catctgcaaa
       61 tgattttatg gctgattagc ggcaacatga aaaattcgct ggaagatgaa agcgcgggag
      121 ttgctggtgt tgtttaagga ttaagcatag gacccacagg ttcgagatat gaaacataaa
      181 agtccgcata tgcacaaaat ggcaccctga tgattctgat agactgcatt gcattataac
      241 cagtagggag gcgaggttag ttatgttttt agtatcattg ttgagacgta ttgcatttag
      301 ctactacgat tataaagctt ataatttcaa tattgaaaaa acagactttg ttgtcatcca
      361 tattcccgat cagattggcg atgctatggc catctttcct gttattcggg cgcttgaatt
      421 gcataaaatt aagcatctat taattgttac gtcgacaatt aatttagaag tctttaatgt
      481 gctcaaactt gaacagacta aattaacatt agtcacaatg actatgcagg atcacgcaac
      541 attaaaagaa ataaaagatt tagcgaagaa cataacacag caatacggta cgccggatct
      601 ttgtatagag gcgatgcgta aaaagaacct gaaaacgatg ttatttatca gtcagttgaa
      661 agcaaaaacg aattttcagg ttgttggttt aaccatgaag tgctattccc ctgtgtgtaa
      721 gaacgcgtcc cggatggatc agaatctccg ggctcccgta cccatgacat gggcatttat
      781 gatgcgtgag gcgggttttc cagcagtcag gccaatatat gaattgccac taagtgagga
      841 tgtactcgat gaggtgcgcg aggaaatgcg atcgttagga tcttacattg cgcttaattt
      901 agaaggtagc tcgcaggaac gtacattttc actatcgatt gcagaaagtc taatagcaaa
      961 aattcaaagt gaaacagata taccaatagt gatcgttcat ggaccaaaag gggaagataa
     1021 cgccagggta ttagtggatt gttataataa tgtctaccgt ttatccttat caccctcgat
     1081 taaacgttca gcagcaatca taaaagatgc ttatatcgca ataactcctg acacttcaat
     1141 attacatatg gc
//
LOCUS       DAJTNA010000399         1150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTNA010000399 DAJTNA010000000
VERSION     DAJTNA010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.406"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(1..1124)
                     /locus_tag="OO161_004347"
                     /pseudo
     CDS             complement(1..1124)
                     /locus_tag="OO161_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          356 a          205 c          174 g          415 t
ORIGIN      
        1 atttttatag ggttctttat cgagattgaa aaataaggtc tcagctattt taattttaga
       61 ttgatcgcag ctaaaatcaa tatgatttat cacggagcat gcatacttta tcttttcaga
      121 atcaccaaaa tattgcattc ttttattaaa acaccaatcc agtattctta catcattaat
      181 atatggggat tttaaaaaag tctccagaat cgatgaccgg tgtaaggtta acttttgcat
      241 catctaagga atcaataaga atatgagaca ggttaacttt gatgtcatta atttcattgt
      301 cagtagcttt cttattatta agaagataat tatttactgc gttaaggtca tctatttctt
      361 tcagtatccc tttttcacca agcaagtatg atatcgattc attacataca taaggcatta
      421 atagcagatg ttctttcctt attgctaata atgcattatt caatggtttt tctaataaat
      481 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
      541 aattatttaa ctcctgcgaa atatttaata gacgagctat ttccttgtta gcttcgcgtt
      601 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      661 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      721 gtccttgacg agtccccata gtagtgaaag tggaaagtgt gatttctatt tccaccttag
      781 ataaatccga tcgttcccct tcaggattat tcataacaaa ctttattatc tcactgccat
      841 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgtttcgt
      901 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      961 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
     1021 tattcatttc caacacgcca ggtatttaaa acttcacttt tttgtttaaa attgtcggct
     1081 aatgtaatgt gttgagaata attcaaatct gatattttgc tcatgacgaa ctccttgagt
     1141 taataagaac
//
LOCUS       DAJTNA010000400         1131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTNA010000400 DAJTNA010000000
VERSION     DAJTNA010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.407"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..849
                     /locus_tag="OO161_004348"
     CDS             <1..849
                     /locus_tag="OO161_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR5465128.1"
                     /translation="WVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNF
                     HQIIMDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIA
                     TYQQYGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVA
                     KLALPSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIA
                     PWTMPTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDI
                     ANALKF"
     gene            900..1131
                     /gene="chbA"
                     /locus_tag="OO161_004349"
                     /pseudo
     CDS             900..1131
                     /gene="chbA"
                     /locus_tag="OO161_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
BASE COUNT          255 a          283 c          305 g          288 t
ORIGIN      
        1 tgggtcatta aactgcccga cagcgtacct gcttcagtat cgcgttcctt ctcggcatta
       61 attcccggct ttattattct ttccgtgatg gggattattg cctgggcgtt gaatacctgg
      121 ggcaccaact tccatcagat cattatggat accatctcaa ccccactggc atcgttgggt
      181 agcgtggtgg gctgggccta tgtgatcttt gttccactgc tctggttctt cggtattcat
      241 ggcgcgctgg cgctgaccgc actggacaac ggcattatga cgccgtgggc gctggaaaat
      301 atcgcgacct atcagcaata tggttccgtc gaagcggcgc tggcagccgg taagaccttc
      361 catatctggg ccaagccgat gctggactcc tttattttcc ttgggggcag tggtgcgact
      421 ttaggcctga tcctggctat ctttatcgcc tctcgccgtg ctgattatcg tcaggtggca
      481 aaactggcgc tgccgtccgg catcttccag attaacgaac cgattctgtt tggtctgcca
      541 attatcatga acccggtgat gtttatcccg tttgtactgg tacaaccgat tctggcggca
      601 atcaccctcg cagcgtacta catgggcatt attcctccgg tgaccaatat tgcaccgtgg
      661 accatgccaa ccggtctggg agccttcttt aacaccaacg gtagcgtcgc tgcattgctg
      721 gtcgcactct tcaaccttgg tatcgcaacg ttaatttatc tgccctttgt tgtggtggct
      781 aacaaagcgc aaaacgcgat tgataaagaa gagagcgaag aagatatcgc taacgccctg
      841 aaattttaat cgatgccggt acggttaatc gtgccggtaa gaagagagag gaacgatgta
      901 tgatggatct cgataacatt cccgatacgc aaacagaagc tgaagagctg gaagaagtgg
      961 tgatggggct tatcatcaac tccggacaag cgcgcagcct ggcgtatgcg gcactgaaac
     1021 aggcgaagta gggcgatttt gccgcagcaa aagccatgat ggatcagtca cggatggcat
     1081 tgaatgaagc gcatctggta cagacgaaac tgattgaagg cgatgcgggc g
//
LOCUS       DAJTNA010000401         1130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTNA010000401 DAJTNA010000000
VERSION     DAJTNA010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.408"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..322
                     /gene="tnpA"
                     /locus_tag="OO161_004350"
                     /pseudo
     CDS             1..322
                     /gene="tnpA"
                     /locus_tag="OO161_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          382 a          236 c          191 g          321 t
ORIGIN      
        1 tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg ctgattttga
       61 agctgaactg gttgaaatgg atggcgaacc agatcacgtc catttgttaa ttaactatcc
      121 tcccaaactg gcaatatcca gtctggtaaa cagcctcaaa ggcgtatcgg gtaggtgact
      181 gcgacgagat ctaccagata ttgcagtcag gtattactac aaaggcgttt tgtggagtcc
      241 tggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc aatacattga
      301 acaacagcaa acacctggtt aggtggaaaa ccacgcctta tatccctgcc ctgaaggacg
      361 gggttttacg gcgcaccgga taagcctgga gattatctac accagcatgg acatcattgt
      421 actatgcccc gtacatgccc catcacatcc tcaacatcaa agtattagag ccttgcaaac
      481 cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa aaaaatagaa ctaatatcaa
      541 gcggcgctat attgcgtatt aaataaaacc acctcagtta cattaataaa tctaaacaaa
      601 catcatgcaa ataatttcca tggttatttc tagggtattt aaattaaata aataaattat
      661 gagtcaatca taaataacaa cctttaatat aatttaacta ccaaatttat attcgttcac
      721 aaaatagtca tgcattgtgt gcaatagaaa cagttcagat aaagataggg atctacttca
      781 caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt ggatgaatat
      841 ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc acgatgaaaa
      901 aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag atttaataac
      961 aacgatatcc gtacctatcc ggatatctaa tgtcataagc atccctttaa ttatcccctc
     1021 aacctcttgt aactttccca ctcttgaact ccacatagtc caaacgccgg atgcaaaaaa
     1081 atcacacgtg catttctgaa attgtgatgg aggaagatag tatcaattat
//
LOCUS       DAJTNA010000402         1129 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTNA010000402 DAJTNA010000000
VERSION     DAJTNA010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.409"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..554
                     /locus_tag="OO161_004351"
     CDS             <1..554
                     /locus_tag="OO161_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5465129.1"
                     /translation="VSGAPAWASEHQSTLSAGYLQPHTDMPGSDDLKGINVKYRYEFT
                     DTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAMAG
                     VAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDL
                     AYEGSGSGDWRTDGFIVGVGYKF"
     gene            618..>1129
                     /locus_tag="OO161_004352"
     CDS             618..>1129
                     /locus_tag="OO161_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5465130.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRKA
                     TAEDVTSTGA"
BASE COUNT          257 a          268 c          370 g          234 t
ORIGIN      
        1 ctgtatccgg tgcgcctgca tgggcgtctg aacatcagtc cacgctgagc gccgggtatc
       61 ttcagcccca tactgatatg ccaggcagcg atgacctgaa gggcattaac gtgaaatacc
      121 gttatgagtt tacggacacg ctggggctgg tgacgtcatt cagctatgcc aacgctgaag
      181 atgagcaaaa aacgcattac agcgataccc gctggcatga ggattccgtg cgtaaccgct
      241 ggttcagcgt gatggcgggg ccgtctgtgc gcgtgaatga atggttcagc gcgtatgcga
      301 tggcgggcgt ggcttacagc cgtgtgtcga ccttctccgg ggattatctc cgcgtaactg
      361 acaacaaggg gaaaacgcac gacgtgctga ccggaagtga tgacggtcgc cacagcaata
      421 cctctctggc gtggggggct ggcgtgcagt ttaacccgac cgaatccgtg gccattgacc
      481 ttgcttatga aggttccggc agtggcgact ggcgcactga cggtttcatc gtgggtgtcg
      541 gttataagtt ctgattagcc aggtaacaca gtgttatgac agcccgctgg ttcaggcggg
      601 ctttttgtgg ggtgaatatg gcagtaaaga tttcaggtgt actgaaagat ggggcgggta
      661 aacctgtcgt aaattgtgcg attgaactgc gggccagaag aaccagtccg accgttgtgg
      721 cacacgttgt tgccacttgc gtgacggaca ataacggtgc ttatgtgatt gaggctgagc
      781 cggggtatta cgaggttgcg cttcactgta acggctggca gccaacccgt gtcggggata
      841 ttgatgtggc accgactgat gcaccgggga cactgaacgc gtttctgaat gcaccaaagg
      901 atggtgattt acgtccggag gtgatgaagc gctttgagga aatggtggcg caggcgcagc
      961 agagcggggc tgcagccgga aacgcacagc agacggcgca ggatgtggcg gcagccgcaa
     1021 cggcccgtga tgatgcacaa cgttttgcgg agaaagcccg acaggatgca accgtcacag
     1081 ctgaggacag aaaggccact gcggaagatg tgacaagcac aggagcaaa
//
LOCUS       DAJTNA010000403         1107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTNA010000403 DAJTNA010000000
VERSION     DAJTNA010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.410"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(183..755)
                     /locus_tag="OO161_004353"
     CDS             complement(183..755)
                     /locus_tag="OO161_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5465131.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATITAGTANADSQFTVEYKK"
BASE COUNT          350 a          234 c          212 g          311 t
ORIGIN      
        1 ccgcgtacca ccaatgacaa cgcctgcata agttaatgct ggttggccta aaaaaacgct
       61 gagtataatc gcggtaaata acttacatga aactgttgtt ttaaagagcc tgattaaaca
      121 actttttctg gacataacga ctccctggcc aaagccaact gaataaaaag gcccttgata
      181 aattatttct tatattcgac agtaaattgt gaatcagcgt tcgccgtacc agccgttatg
      241 gtcgcattgg ttgccacata gcgtgcaata aacttcattt cagccttgcc ttcagcatcg
      301 atagcggtat aagcagagtc gttataaagt ttaacctgcg aattatcgga ctggttaaaa
      361 atacgaatac caacgccggt tgccgcaatc gcttgtcctg taccggtaaa atcaccaggg
      421 ttgcctgctg taagcggcgt acccactttc aggtcgccgt cgccgccagg cgcttctgta
      481 ccatcaaaac gaacggctgc ttgagtgtac gttgccgggc aattttcaag tttaatactg
      541 aacttctgtg gcgacgccag actacctaca cctgaaaatg catttttact gactttaccc
      601 agtgggacaa tttgatcggc tgtatcggag gtaacttcac aagtagtgtc gacaatttca
      661 ccgacgaagt gaacaacacc atcttctgcc agtgcatgcc cggccagcat tagagatgcc
      721 agagtgaatg caccgcctat aatattacgc ttcataaaaa caccattgat ataatatatc
      781 ctttaccatg aatcatcact tatattaata agtgtatttt tgattcaaag gaatagtaca
      841 ccaaagagac ggctttatct aaaaataata ttaattacgc aaaaataaaa tataaattta
      901 ttgtttaaat attatttaaa cagtaaaaaa tcaagtttgt tatcagacat taatgataat
      961 tcagaatata tttatataga aatatagatg aatttattga tgtcagttat aaaaaagggc
     1021 caccggtcgg cagtaatgcc gatcagttaa ggatcagttg accgatccag tggctgtgta
     1081 agaatccgga aacgctcact tgtttcc
//
LOCUS       DAJTNA010000404         1096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTNA010000404 DAJTNA010000000
VERSION     DAJTNA010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.411"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            204..623
                     /locus_tag="OO161_004354"
     CDS             204..623
                     /locus_tag="OO161_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5465132.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKSQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            623..1096
                     /gene="umuC"
                     /locus_tag="OO161_004355"
                     /pseudo
     CDS             623..1096
                     /gene="umuC"
                     /locus_tag="OO161_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
BASE COUNT          270 a          232 c          287 g          307 t
ORIGIN      
        1 ttctattcta tttttttgcc gggtgatatt tttcatttct gctggatgag cgtcgtcgcc
       61 agaaggccac gtgagcacag gataagagaa cgaaaaatca gcagtctata cagcgacaaa
      121 tattgatagc ctgaatcagt attgatctgc tggcaagaat agactactgt atataaaaac
      181 agtataactt caggcagatt attatgttgt ttatcaagcc tgcggatctc cgcgaaattg
      241 tgacttttcc gctatttagc gatcttgttc agtgtggctt tccttcaccg gcagcagatt
      301 acgtcgaaca gcgcatcgat ctgaatcaac tgttgatcca gcatcccagc gcgacttact
      361 tcgtcaaagc aagtggtgat tctatgattg atggtggaat tagtgacggt gatttactga
      421 ttgtcgatag cgctattacc gccagccatg gtgatattgt catcgctgct gttgacggcg
      481 agtttacggt gaaaaaatcg caactacgcc cgacggtaca gcttattccc atgaacagtg
      541 cgtactcgcc cattaccatc agtagcgaag atacgctgga tgtctttggt gtggtgatcc
      601 acgtcgttaa ggcgatgcgc tgatgtttgc tctctgtgat gtaaacgcgt tttatgccag
      661 ctgtgagacg gtgtttcgcc ctgatttatg gggtaaaccg gtggttgtgc tatcgaataa
      721 tgacggttgc gttatcgccc gaaacgctga ggcaaaggcg ctgggtgtta aaatgggcga
      781 tccctggttc aaacaaaaag atctgtttcg tcgctgtggt gtggtttgct ttagcagcaa
      841 ttatgagctt tacgcagaca tgagcaatcg ggtgatgtcg acgctggaag aactatcgcc
      901 ccgcgtcgag atttacagta ttgatgaggc attctgcgat ctgacaggtg tgcgtaattg
      961 ttgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca tcttactgtt
     1021 ggtgtgggga tcgcccagac caaaacgctg gctaagcttg ccaatcatgc ggcaaaaaaa
     1081 tggcagcggc agacgg
//
LOCUS       DAJTNA010000405         1094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTNA010000405 DAJTNA010000000
VERSION     DAJTNA010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.412"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..552)
                     /locus_tag="OO161_004356"
     CDS             complement(<1..552)
                     /locus_tag="OO161_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5465133.1"
                     /translation="MLEKSLATLFVLLILATLINRFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNC
                     FELFLLLIPLAGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPS
                     MNIQGGALLVLFLLALTASNDIAQ"
     gene            complement(552..>1094)
                     /locus_tag="OO161_004357"
     CDS             complement(552..>1094)
                     /locus_tag="OO161_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5465134.1"
                     /translation="YKHHVTANHITLTALALSLFTGLLLVLVAQPILFLLLPIVLFIR
                     MALNALDGMLARECNQQTRLGAILNETGDVISDIALYLPFLFLPESNASLVILMLFCT
                     ILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGLAVAIYPQWMQWNNLLWSIASI
                     LLLWTAINRCRSVLFMSAER"
BASE COUNT          385 a          266 c          223 g          220 t
ORIGIN      
        1 ctgtgcaata tcgtttgacg cggttaatgc caaaagaaat aacaccagta aggccccgcc
       61 ctggatattc atggatggca acattaataa ccaggcggca tgactcaagg cgaaaacggt
      121 cattatccag ccccaaaaaa tggcgctcac ggtatgcaga aaaccggagg ggtcacccac
      181 aaaaactcgc caggtggcta atatcagaaa cccagccaaa ggtattaata acagaaacag
      241 ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat ataaccaccg
      301 cggaaaatgt acggatataa gcatacaata ttctttcaat gccagaaaac ttatcagcgc
      361 aaaaaacgcc aacgtcatcc agcgtggacc tgaaatcacc agtgaaaaac aaatgacgat
      421 gccccaccag gtacgaatac gtaatgtaac ctcaccccct tttctctccg gtaaccgcca
      481 tagcagaaaa cggtttatca gcgttgctaa aattaacaac acgaaaagtg ttgccagaga
      541 tttttccagc attatctttc agcactcata aaaagcacac tccgacaacg attaatcgca
      601 gtccagagaa gcagaattga agcaatgctc cataaaagat tattccattg catccactgt
      661 ggataaatgg caacggctag cccccacaga ccaaatatta acgcgcgatc gcttttgccg
      721 aatggaccgg cataactgcg aataccgtta atcgtctgcg cgagtaaacc gcaaaactca
      781 gtcaatatgg tgcaaaataa catgagtatt acgagtgatg cgttactttc cggtaaaaat
      841 aaaaacggta aatagagcgc aatatcggaa ataacatcgc cagtttcatt caaaatcgcc
      901 cccagacgtg tttgctgatt gcactcacgc gccaacatgc catccagcgc attgagcgcc
      961 atacggataa aaagcacgat gggcaatagc aaaaagagga tgggttgtgc caccaacacc
     1021 aacagcaatc cggtaaaaag agaaagcgcc agtgccgtaa gagtgatgtg attcgctgta
     1081 acgtggtgct tata
//
LOCUS       DAJTNA010000406         1085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTNA010000406 DAJTNA010000000
VERSION     DAJTNA010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.413"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            312..>1085
                     /gene="kgtP"
                     /locus_tag="OO161_004358"
     CDS             312..>1085
                     /gene="kgtP"
                     /locus_tag="OO161_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5465135.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLC"
BASE COUNT          262 a          255 c          262 g          306 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgcc tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aaatttatcc ttaagctcct caataaccat
      181 tttcctgcta actaaattca tggttaatgt tgcataataa tacgcaacaa atgtatcaca
      241 ttttctttac caaaagaaat aaacaaaagc gaccaacaaa agcatcggat tacggcagga
      301 gacataatgg catggctgaa agtactgtaa cggcagacag caaactgaca agtagtgata
      361 ctcgtcgccg catttgggcg attgtggggg cctcttcagg taatctggtc gagtggttcg
      421 atttctatgt ctactcgttc tgttcactct acttcgccca cattttcttc ccttccggga
      481 acacgacgac tcaactacta caaacagcag gtgtttttgc tgcgggattc ctgatgcgcc
      541 caataggcgg ttggctattt ggccgcatag ccgataaaca tggtcgcaaa aaatcgatgc
      601 tgttatcggt gtgtatgatg tgtttcggat cgctggttat cgcctgcctc cctggttatg
      661 aaactatagg tacgtgggct ccggcattat tgcttctcgc gcgtttattt cagggattat
      721 ccgttggcgg agaatatggc accagcgcca cctatatgag tgaagttgcc gttgaagggc
      781 gcaaaggttt ttacgcatca tttcagtatg tgacgttgat cggcggacaa ctgctagccc
      841 tactggttgt cgtggtttta caacacacca tggaagacgc tgcactcaga gagtggggat
      901 ggcgtattcc tttcgcgtta ggagctgtgt tagctgttgt ggcgttgtgg ttacgtcgtc
      961 aattagatga aacttcgcaa caagaaacgc gcgctttaaa agaagctgga tctctgaaag
     1021 gattatggcg caatcgccgt gcattcatca tggttctcgg ttttaccgct gcgggctccc
     1081 tttgt
//
LOCUS       DAJTNA010000407         1081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTNA010000407 DAJTNA010000000
VERSION     DAJTNA010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.414"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..772)
                     /locus_tag="OO161_004359"
     CDS             complement(<1..772)
                     /locus_tag="OO161_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5465136.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSH"
BASE COUNT          310 a          155 c          253 g          363 t
ORIGIN      
        1 catgggatga caatgggtta tcactaatat ctattgaaca ttcattcctc agattaagaa
       61 tgctttccgg gatatgactt atctgattta tgtcaaaata accgttcctt aaattttccg
      121 gcaggtatgg aatatcggtt aacatattgt cgattgccca tatggagtga atgttgtgag
      181 gtagaggggg gagttcacta agaagattcc ctattgcgct aatctcttgt aaagaaaatg
      241 gtaaaggtgg taaaactgcc agcccattac atgatacatc taatgtttcc agtagttctg
      301 gtaaaacagg aagagaacat aattgattat ttgagacatg aagctccttc aaagatatgg
      361 ggagtgtggg tagtgtgatt agttgattgt gggacgcatt caataattta agtccttgag
      421 gcaaagcagg cagttcaata agtctgttat agctgacatt aagctgtgta agggacgcag
      481 gcaatgggga gattaagctt aaattatttt tacttatatt aattgattta attcccgggg
      541 ggatttcagg taacgttgtc aggcctaatt cagacaagtc taggttcgtc tcttggtttt
      601 gtagacatga tactagtcgc tgaaaagcga tgtctcgttg ttcttcttgt atgcggttat
      661 ttttccattc agtccaatgg gttagataat tttcatatgc gcggctagtg tcgattgaat
      721 aagtggagaa cgaatttgaa attaatctgt gattgttatt tgtcgggagc ataaatatca
      781 gggcgtattc tttatgttga gaggtgttgg attctttttt atctgctttg caatgttatt
      841 ggttcccttc agcagtagac aggaacttct ggaggaggcg gatatatagt ttgtgtaagc
      901 atattaactc catatgttat atattgaaga acttctgctt tacactatcc taaactggat
      961 cggtttaatg taaaaaaacg atactttttg ggggaggggg caatttttcc ttaaggtatg
     1021 aaaaaaggag ctgaagctat ataatagcta ttaatgccac tggtatcagt gaatcatgta
     1081 t
//
LOCUS       DAJTNA010000408         1069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTNA010000408 DAJTNA010000000
VERSION     DAJTNA010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.415"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..176)
                     /locus_tag="OO161_004360"
     CDS             complement(<1..176)
                     /locus_tag="OO161_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5465137.1"
                     /translation="MIVLILVFRLVIGVQMIDVLGPEKRRRRTTQEKIAIVQQSFEPG
                     MTVSLVARQHGVAA"
     gene            375..593
                     /gene="ccdA"
                     /locus_tag="OO161_004361"
     CDS             375..593
                     /gene="ccdA"
                     /locus_tag="OO161_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5465138.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            595..900
                     /gene="ccdB"
                     /locus_tag="OO161_004362"
     CDS             595..900
                     /gene="ccdB"
                     /locus_tag="OO161_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5465139.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            936..1031
                     /locus_tag="OO161_004363"
                     /pseudo
     CDS             936..1031
                     /locus_tag="OO161_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          291 a          228 c          265 g          285 t
ORIGIN      
        1 ctggctgcta caccatgttg ccgggcaacg agggagaccg tcatccccgg ttcaaagctc
       61 tgctgaacaa ttgcgatctt ttcctgtgtg gtacgccgtc tgcgtttctc cggccctaag
      121 acatcaatca tctgtactcc aatgactagt ctaaaaacta gtattaagac tatcacttat
      181 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagtatgcta attatcgtat
      241 aaaaaaatcg cagcagaaaa aattgtatta aaaacttata gtgaattaat ggtgtacttt
      301 ttatggatat tgatggtttg taccattaat atatactcat atacatatca taagtatgtt
      361 tttgagggct tatcatgaag cagcgtatta ctgtcactat agacagtgac agctatcagt
      421 tacttaaatc cgcaaacgtg aatatctcag gcctggtaaa tacggccatg caaaaagagg
      481 cccgccgtct gcgtgcggaa cgctggcagg ctgaaaatca gcaggggatg gctgagatcg
      541 cccgttttat tgaaatgaac ggctcttttg ccgatgagaa cagggactgg tgaaatgcag
      601 tttaaggttt acgcctataa aagagagagt cgttatcgtc tgtttgtgga tgtacagagt
      661 gacattattg acacgcccgg acgacggatg gtgatccccc tggccagtgc ccgcctgctg
      721 tcagataaag tctcccgtga gctttacccg gtggtgcatg tcggggatga aagctggcgc
      781 atgatgacca ctgatatggc cagtgtgccg atcttcgtta tcggggaaga agtggctgat
      841 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
      901 atgtcaggct agggtcaaaa atcgtggcgt tgacaaaatg gctgcgttac gtcattgagc
      961 atatccaaga ctggccggca aaccgggtac gcgatctgtt gccctggaaa gttgatctga
     1021 cctctcagta aatatcaata cggttctgac gagccgctta ccgttcaaa
//
LOCUS       DAJTNA010000409         1038 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTNA010000409 DAJTNA010000000
VERSION     DAJTNA010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.416"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(333..809)
                     /locus_tag="OO161_004364"
     CDS             complement(333..809)
                     /locus_tag="OO161_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5465140.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          258 a          256 c          247 g          277 t
ORIGIN      
        1 ggtcaagcgc gccgataccc ctaaccaggg gctttcaggt cgccaggaac cgcgccagcc
       61 ccctgcaaag cccatttgca cggaacaggc agccatgcgg ttggtaggtg gtcgtagacc
      121 gtcggtgttg cagcgatggg ggtctggggt tctccccaga cggtttccgg tagcgctcaa
      181 ggcgctgccg gaaacgtggc ggctcggttt tttctggaga acggggagca aaaacacgca
      241 aaggattttg aggcggcaaa agagcaatgc aggatagaca atcatggtca gaacgttaac
      301 acgtagcccc tgattgtccg tctgccgcta gctcaatttg aaaacatggc catgattcgg
      361 cgctgaatat caccgtcttt gttgaccagt gttttagtga caaacgctgc atggtttttc
      421 gtagtgttgt atcccttcca gacttcatgt ggatcgaggg catacgcaca aaccttacca
      481 gcaacgggga ttctaactaa tgcatttttt gattctaagt aattgagcca ccgaataatt
      541 gtgcttctgc taactccaag tttttcagct aggaattgct gatccgcaac gacagcgcca
      601 cacgtaggat cgatatgctc cgctaaaaat gagtacagct tcagtgctga tatgccttgg
      661 ctatccttca gcagctctcg aacacgttcc caaccttttg gggatacctg tgtaaacctt
      721 gaatttttct ttgcttcttc gagatcctcg gtgtttttct ttgcctcatc tgaaagttgt
      781 ttcagtcgtt tttctgaatc aataaacatg attttttccc tctgaaaatc ccatgcacta
      841 acgttggttc aaatctaagc catcatgaca catctgtgca acaccttgtt gcgtgttgtc
      901 gcatccagga tgcgacaacg ttgtgtcatc ctggatgcga caggtaaata aaaaacacac
      961 taaaaaaaca accacttaca aatcggcgta aaagatctat tctaggcaag ggtgttgcgt
     1021 gcaacgcaac gccctttg
//
LOCUS       DAJTNA010000410         1029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTNA010000410 DAJTNA010000000
VERSION     DAJTNA010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.417"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            6..851
                     /locus_tag="OO161_004365"
     CDS             6..851
                     /locus_tag="OO161_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5465141.1"
                     /translation="MEHLLKSESTALRESIYAVIAGKDCYQADTLSLLPGVKIISRFG
                     TGIDNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGE
                     SHGYELQGKRIGMVGYGNIGKTLAKRLSGFDVELLAYDKQPDYQVADKVGVQFVSIED
                     IFMQSHVIIVLLPFSPELENFISHKYLSMMRNGALIINAARGKLLDEGALLQVIEERN
                     VFAALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGTHVAQSIIDYFAGRE
                     IKNVL"
     gene            complement(930..1006)
                     /locus_tag="OO161_004366"
     tRNA            complement(930..1006)
                     /locus_tag="OO161_004366"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(970..972),aa:Met,seq:cat)
BASE COUNT          281 a          210 c          250 g          288 t
ORIGIN      
        1 ataatgtgga gcatctgctt aaatcagaat ccactgcatt acgagaatct atttacgcgg
       61 tcatcgccgg aaaagattgt tatcaggcgg acacgctatc attgctgcct ggtgtgaaaa
      121 taatatcgag attcgggacg gggatagata atattgatct tcgtgcagca caacaatccg
      181 gtattgtagt caataatgcg gttgggatta attcaaatgc tgtcgcggaa tttatcattg
      241 gacttatctt cgcgagcatg agaaatattc ccggcagcta tcatgcgatg caaaatggct
      301 actggggtga gtcgcatggc tatgaattac aaggcaaacg gattggcatg gttggctacg
      361 gcaatatagg taaaaccctg gcaaaaaggt tatccggttt tgatgttgag cttttagctt
      421 atgacaaaca acccgattat caggttgctg acaaagttgg agttcagttt gtatcaattg
      481 aagatatctt tatgcagtcg catgtcatca ttgttctttt gcctttttct cctgagctgg
      541 agaacttcat tagtcataaa tatttgtcga tgatgcgcaa tggtgctcta atcatcaatg
      601 ccgcaagggg aaagttgctg gatgagggcg ctttactaca ggtgattgaa gaacgaaatg
      661 tgtttgcggc gctggatgtg tttagctctg aaccgttggc gcaatttagc ccactgctac
      721 atgccaaaaa tatcattaca acgccgcata ttgcggccgc caccgttgag tcataccagc
      781 aaaccggtac acacgttgcc cagtcgataa ttgattactt cgcaggaagg gagataaaaa
      841 acgtgttgtg agttcagcga acgaagtttt taaatacaaa aaaaacgcaa tttgtggaac
      901 acattgcggt ttatcagcat tctaactatt ggttgcgggg gccggatttg aaccgacgac
      961 cttcgggtta tgagcccgac gagctaccag gctgctccac cccgcgtcac cgtactgctt
     1021 tatttcatc
//
LOCUS       DAJTNA010000411          987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTNA010000411 DAJTNA010000000
VERSION     DAJTNA010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.418"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..135
                     /locus_tag="OO161_004367"
     CDS             <1..135
                     /locus_tag="OO161_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="biosynthetic; catalyzes the formation of trehalose
                     and phosphate from trehalose-6-phosphate; expression is
                     increased under osmotic stress and induced during the
                     transition to stationary phase and by decreased
                     temperature; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5465142.1"
                     /translation="ATQASWRLAGVPDVWSRLEMITTALQQKRENNRSDDYESFSRSI
                     "
     gene            110..>987
                     /locus_tag="OO161_004368"
     CDS             110..>987
                     /locus_tag="OO161_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCR5465143.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLMQFQRPA
                     GDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     NEALLEKYPQHHGKIR"
BASE COUNT          266 a          241 c          246 g          234 t
ORIGIN      
        1 gcaactcagg catcatggcg actggcgggt gtgccggatg tctggagcag gcttgaaatg
       61 ataaccaccg cattacaaca aaaaagagaa aataacagga gtgatgacta tgagtcgttt
      121 agtcgtagta tctaaccgga ttgcaccacc agacgagcac gccgccagtg ccggtggcct
      181 tgccgttggc atactggggg cactgaaagc cgcaggcgga ctgtggtttg gctggagtgg
      241 tgaaacaggg aatgaggatc agccgctaaa aaaggtgaaa aaaggtaaca ttacgtgggc
      301 ctcttttaac ctcagcgaac aggaccttga cgaatactac aaccaattct ccaatgccgt
      361 tctctggccc gcttttcatt atcggctcga tctgatgcaa tttcagcgtc ctgccgggga
      421 cggctatcta cgcgtaaatg cgttgctggc agataaatta ctgccgctgt tgcaagacga
      481 tgacattatc tggatccacg attatcacct gttgcccttt gcgcatgaat tacgcaaacg
      541 tggggtgaat aatcgcattg gtttctttct gcatattcct ttcccgacac cggaaatctt
      601 caacgcgctg ccgacatatg acaccttgct tgaacagctt tgtgattatg atttgctggg
      661 tttccagaca gaaaacgatc gtctggcgtt cctggattgt ctttctaacc tgacccgcgt
      721 cacgacacgt agcgcaaaaa gccatacagc ctggggcaaa gcatttcgaa cagaagtcta
      781 cccgatcggc attgaaccga aagaaatagc caaacaggct gccgggccac tgccgccaaa
      841 actggcgcaa cttaaagcgg aactgaaaaa cgtacaaaat atcttttctg tcgaacggct
      901 ggattattcc aaaggtttgc cagagcgttt tctcgccaat gaagcgttgc tggaaaaata
      961 tccgcagcat catggtaaaa ttcgtta
//
LOCUS       DAJTNA010000412          972 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTNA010000412 DAJTNA010000000
VERSION     DAJTNA010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.419"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>972
                     /locus_tag="OO161_004369"
     CDS             <1..>972
                     /locus_tag="OO161_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5465144.1"
                     /translation="EHLPLYRQSEIFARQGVELSRALLSNWVDACCQLMTPLNDALYR
                     YVMNSRKVHTDDTPVKVLAPGRKKAKTGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQG
                     KHPEQHLSPFRGILQADAFNGYDRLFSAEREGGALTEAGCWAHARRKVHDVYISTKSA
                     TAEEALKLIGELYAIEHEIRGLPVSERLAVRQMQSKPLLTSLYKLMQEKEHTLSKKCR
                     LRDAFRYIRKHWVALCNFSDDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGA
                     LLYGLIGTCRLNGIDPEAYLRYILSVLPEWPSNRVDELLPWNVALTN"
BASE COUNT          241 a          242 c          284 g          205 t
ORIGIN      
        1 cgaacacctg ccactgtatc gtcagagtga aatttttgcc cgtcagggtg tcgaactgag
       61 ccgtgcatta ctctccaact gggttgacgc gtgctgccag ttaatgacgc cgctgaatga
      121 tgctctgtac cgttatgtga tgaacagccg caaagttcac actgatgaca caccagtaaa
      181 agtgctggca ccgggcagga agaaggcgaa aacaggatat atctggacgt atgtccggga
      241 tgacaggaat gccggttcgc cagagcctcc ggcggtctgg ttcgcctact caccggacca
      301 tcagggtaaa catccggagc agcaccttag tcccttccgg ggtatcctgc aggcagatgc
      361 gtttaatggt tacgatcggc tgttcagtgc cgaacgagaa ggcggcgcgt tgacggaagc
      421 aggatgctgg gctcatgcgc ggcgcaaagt ccacgatgta tatatcagta ccaaaagcgc
      481 gacagcggaa gaagccctga aactaatcgg tgagctgtac gccatcgagc acgaaatacg
      541 cgggttgccg gtgtctgaac gcctggcggt caggcaaatg cagagtaaac cgctactgac
      601 ttccctgtat aagctgatgc aggagaaaga acacacgtta tcgaaaaaat gccgtctgag
      661 agatgcgttc cggtatatca ggaagcactg ggttgcgttg tgcaacttca gtgatgatgg
      721 tctggctgag gcggataata atgccgcgga aagagcgctt cgtgcagtct gtctcggaaa
      781 gaaaaacttt atgttcttcg gcagcgatca cggtggagag cgtggtgcgc tactgtacgg
      841 gctgatcggc acctgccgac tgaacggtat cgatccggaa gcgtatctgc gctatatcct
      901 gagcgtactg ccggaatggc cttccaaccg tgttgacgaa ctcctgccat ggaacgtagc
      961 actcaccaat aa
//
LOCUS       DAJTNA010000413          969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTNA010000413 DAJTNA010000000
VERSION     DAJTNA010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.420"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            8..>969
                     /locus_tag="OO161_004370"
     CDS             8..>969
                     /locus_tag="OO161_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCR5465145.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLKSFLLVHGVHY
                     VGRADWGPAHRRWLSKYSFESPWRQLAFDEHRRTIEDRQAQCERLESALKEAVTEWRL
                     YPVVEALQAMRGIQFITAVGLISELGDLTRFEHPRQLMSWFGITPSEYSSGGSRHQGS
                     ITKAGNSYARKLLVEAAWSYRHPARISPAIQKRQENLPRPVIDRA"
BASE COUNT          245 a          233 c          259 g          232 t
ORIGIN      
        1 ggccagcatg aacaacaata acacattgta cgtcgggtta gatgttcaca aagaatcgat
       61 tactgtcgct tatgctatca attcagaacc tgttgaactg atgggtaaaa ttggcacatc
      121 acctactgat attcagaatc tttgtaaacg tctcaggtca aagtcatcgc aggtcagtat
      181 cgtttatgaa gcggggccct gtggttatgg actttatcgt cggctggtga aatccggttt
      241 tgactgcatg gtctgcgccc cctcacttat cccgaaaaaa ccgggggagc gggttaaaac
      301 cgatcgccgt gatgccatca gacttgtgcg ttcactgcgt gcaggagatc tttctgctgt
      361 ctacgtaccc ggcattgaag atgaggcatt ccgggatttg gcccgggcat gggcatctgc
      421 ccgcgatgat ttacggcatg caaggcaacg tctgaaatcg tttcttctgg ttcatggggt
      481 ccattatgtc gggcgagcag attggggccc tgcacaccga cgctggctca gcaaatactc
      541 attcgaaagt ccctggcgac aattagcttt tgacgaacac cgtcgtacaa tagaagaccg
      601 gcaggcacaa tgtgaacggc tggaatccgc cctgaaggag gccgttaccg aatggaggct
      661 gtatccggta gtagaggcgc tgcaagccat gcgagggatt cagtttatta ctgcggttgg
      721 attaatttct gaactgggtg atctgacccg ttttgaacat ccacgacaac tgatgtcatg
      781 gtttggtatc acgccatcag aatattcatc gggtggaagc agacatcagg gcagcattac
      841 caaagccgga aacagctatg ccagaaagct attggtcgag gcagcgtgga gttaccgtca
      901 tccggcacgc atcagtccgg caatacaaaa aaggcaggaa aatttacccc gccccgtcat
      961 tgacagagc
//
LOCUS       DAJTNA010000414          948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTNA010000414 DAJTNA010000000
VERSION     DAJTNA010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.421"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            231..902
                     /gene="queE"
                     /locus_tag="OO161_004371"
     CDS             231..902
                     /gene="queE"
                     /locus_tag="OO161_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5465146.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWHLSMQTHK
                     YLNIA"
BASE COUNT          268 a          197 c          226 g          257 t
ORIGIN      
        1 ttatcattaa aaactaatac agaaaaaata aatataattt tggtgaatgt tttgacatga
       61 ttaaatccat tttctttatt ttcttctgac ataatacgtt actcccttta attattaacg
      121 atatgtttcg catttttctt taaatctgca tgtaacagaa tgttaataag acatgattgt
      181 tgcggcagga tctgcaataa ttaacaatta tccacttcac agagaatgct atgcagtacc
      241 cgattaacga gatgttccag accctgcaag gtgagggtta ctttaccggc gtccccgcca
      301 tttttattcg tttacaggga tgcccggttg gctgtgcctg gtgcgacacc aaacacacct
      361 gggaaaagct tgaggatcgg gaagtctccc ttttcagcat tctggcgaag accaaagaga
      421 gtgataagtg gggggcggcg agcagtgaag atttgctggc tgtcattggt cggcagggat
      481 ataccgcgcg gcatgtggtg attacgggtg gtgagccttg cattcatgat ttgctgccac
      541 tgactgacct gctcgaaaag aacggtttta gctgccagat cgaaaccagt ggaactcatg
      601 aagtacgctg cacaccgaat acctgggtta ccgtatcgcc aaagctgaac atgcgcggcg
      661 gctatgacgt gttgtcacag gcactggagc gagccaacga aatcaagcat ccagtggggc
      721 gcgtacgcga tattgaagca ctggatgaac tattggcaac gctgaccgat gataaaccgc
      781 gagtcattgc actgcagccg attagccaaa aggatgatgc cacacgtttg tgcattgaaa
      841 cctgcattgc gcgtaattgg catttgtcga tgcaaacaca taaatatcta aatattgcct
      901 gattaaacat ttataagcgt tataaatggg tggaacctgt aaagactt
//
LOCUS       DAJTNA010000415          928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTNA010000415 DAJTNA010000000
VERSION     DAJTNA010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.422"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            56..>928
                     /gene="pgrR"
                     /locus_tag="OO161_004372"
     CDS             56..>928
                     /gene="pgrR"
                     /locus_tag="OO161_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5465147.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDNQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFID"
BASE COUNT          244 a          200 c          225 g          259 t
ORIGIN      
        1 caatcatgat tagacctatc atttaaatga ttaatagatt gcatggagat actgaatgaa
       61 gcgtgaagaa atcgctgatc tgatggcgtt tgtcgtcgtt gcagaggagc gtagcttcac
      121 tcgtgcagca gcccgcctga gcatggcgca gtcagcttta agccagatag tgcgtcgtat
      181 agaagaacga ttgggattgc ggcttctgac gcgaaccacg cgcagcgttg ttccaactga
      241 agcgggcgag catcttttgt ctgttcttgg ccctatgttg catgacatag attcagccct
      301 ggcatccctg agcgagctgc agaaccgccc atccgggaca atacgtatta ctactgtaga
      361 acatgcagca aaaacgatat tgttaccagc aatgcgcaca ttcctgaaat cgcatcctga
      421 aattgatatt cagctcacca ttgattatgg tttgaccgat gtcgtttctg aacgttttga
      481 tgcaggcgtc cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc
      541 agatatacca atggctattg ttggctcacc ggattatttt tctcgccgaa gtgttccaac
      601 gtcagtgtca caattaatag ataatcaggc aattaatttg tatcttccca catcgggtac
      661 agcaaatcgc tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca
      721 gcttttactg aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc
      781 gtatctacct tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt
      841 ggataaattc acaccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc
      901 tggctcggca ttctcattat ttatagat
//
LOCUS       DAJTNA010000416          928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTNA010000416 DAJTNA010000000
VERSION     DAJTNA010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.423"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..587
                     /locus_tag="OO161_004373"
     CDS             <1..587
                     /locus_tag="OO161_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5465148.1"
                     /translation="ANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYR
                     LEKKISSRPVEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVE
                     LSRFLEDGAVPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGL
                     EPHAWLTDVLMRLPEWPEERLAELLPLEGFTFSG"
BASE COUNT          222 a          255 c          273 g          178 t
ORIGIN      
        1 tggcaaacaa agtgccggag atcacgctgg ccggatgctg ggcccatgcc cgcaggggct
       61 tcgccgacct gtataaaatc agtaaagatc cacgggctgc catagccgtg aagaaaatcg
      121 cggggttgta ccgtcttgag aagaagatca gtagccgccc cgtggaaaaa atccgccagt
      181 ggcgacagcg ttatgcccgt ccgatactgg aagaactgtg gtcatggctt gaagagcagg
      241 aaccgcaatg ttctccggga aaggcattac acaaagccat tgcctatgcg ctgtctcatc
      301 gcgtggaact gagccgcttc ctggaagatg gtgcggtgcc gctggataat aatgtgtgtg
      361 aacgggccat caaaaacgtg gttctgggca gaaaatcgtg gctgttcgcc ggttcgcaga
      421 tggcgggaga acgcgccgcg caaataatga gcttgctgga aaccgcgaaa cgcaacggtc
      481 tggagccgca tgcctggttg acagacgtcc tgatgcgtct gccggagtgg ccggaggagc
      541 gactggcaga gttgctgcct cttgagggat ttaccttctc cgggtgagtg atacctgccg
      601 tcaggtgttc gtgcaccggg ccataacctg cagtcgggag ttgaactcct gacggcagga
      661 aatgagccag accagcaacc acgcgggctg acagcgaata agaacctgaa tcagcccgga
      721 gtcattcccg ggtcagtaca tcatcacctg caattaatcc ccgagtcagt acttcgcggc
      781 ctgacgcgat cacgctactc agtacttcag agcccgcctc cggtacctgc ctggcgatcg
      841 cctctgtgca acaaaataaa tcaaccgtta tcaaaccacg agcgttcgta tgcgccgccg
      901 atgacgcgcg cctgaaaaac gcgtcaat
//
LOCUS       DAJTNA010000417          915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTNA010000417 DAJTNA010000000
VERSION     DAJTNA010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.424"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(93..461)
                     /locus_tag="OO161_004374"
                     /pseudo
     CDS             complement(93..461)
                     /locus_tag="OO161_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          275 a          221 c          163 g          256 t
ORIGIN      
        1 gcattgacca agccgtaagg cacggtaggt aattgcaggt atccgacaat ttcgagtaga
       61 ggatccacta tttcactact tagaattcaa aatcagaatc ctttcttcct gctaaggcta
      121 tctctgaatg actcaaccac tctttgagat tcttcttctg ctcacatgaa gtcattcccg
      181 gacagaaaaa aaccattgtc aggcccactg gcttcagaga aattcgtttc tgcagcagac
      241 acagaatcca catcagcaag agacggttca tctgtacttt ttctcaatct gtaaagagcc
      301 ttatcaaacg caaccagtga gatattcagg ccgtaacgtt cattcagcac agtcacaatc
      361 tcttccctac ggataccatt tttcttccag gtttctatat cgttcttgga atccctgaca
      421 gccgcaatgg ctgttcttgg accagggtta aaattcgcca tataccgccc ataatgtctt
      481 aaatttgtct cataatagtc caaaaataag attgaaacgc aaaaaatgtc ttacaattat
      541 cttattgttg tcctttccgg ttcatgtatc catgaaccgc ctgatgtgga acgcttttta
      601 tcgatctcat gcgcagccaa agatagattc attctttaaa agtcaggtta cattatccct
      661 taaatggtca tacaccatac gcaaccgcga ttttcatcgc aattgcatga attaaaaggt
      721 aaaaactgaa gtccccgttt atttatgtaa tacgcaacgt attacaacca cctgtaatta
      781 catgaaaata aagaaataag atgtaatacc ggcgtattac cacgtacgcg ggtaagcgaa
      841 aaagctataa cgcttcccgg ccgacaggcg gcccaaaatc caccggggcg tgcaggttct
      901 cttcagtaac ctgca
//
LOCUS       DAJTNA010000418          908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTNA010000418 DAJTNA010000000
VERSION     DAJTNA010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.425"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..230)
                     /locus_tag="OO161_004375"
     CDS             complement(<1..230)
                     /locus_tag="OO161_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5465149.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFT"
     gene            complement(211..624)
                     /locus_tag="OO161_004376"
     CDS             complement(211..624)
                     /locus_tag="OO161_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5465150.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(651..>908)
                     /locus_tag="OO161_004377"
     CDS             complement(651..>908)
                     /locus_tag="OO161_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5465151.1"
                     /translation="LVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
BASE COUNT          210 a          285 c          220 g          193 t
ORIGIN      
        1 gtaaactgtg cgggcagggt tcgcatggcc gctttatact gcccgacgga aatcccggct
       61 ttttgtgcag ccagcgcctg gcggctcagg ccctgttcaa cagcactggc ggtttttctg
      121 gcgtcggtat ccagacctga aaaatgacgc cttacccggc tcatctgctc atcgaaacgg
      181 acagcatcca gactcaggtc aataacaaga tcaccaaccg gctgggacat atctcacacc
      241 tcccggaatc cccgctgaag ccatcattaa tgcggcatca tccaccatga catccgccac
      301 atccgcagac gataaaatat cgcgccctcc gtccccaccg aaccggacgc ctccggcaag
      361 tcctgccgct ttctgcatca gcattttgtc ctcatccggc ctctccacct gctcttcctc
      421 atgccggggg acaagcagac tgaaatcaga gggatgcata tccggatcgc aaaaaaacag
      481 gctgagtaca gcgtacgtca gcccggaaaa atgcatatcc agctgggtat cctgaaaata
      541 atgcgtgcgg taaaaacggt gccagtcggc atattcggtg gatgtcatcc cggcaagcat
      601 ggcgcgccag tcgggtctcc ccatctcacg cgccagtctg agggcaaagt tcagctcgcc
      661 gtcgaagact ttcccgcaga aaaatcatca tcagtcagcg tgttattttt cgccacttca
      721 gtaatatcag tatccggacg aacagcttcg atcatcccgg acaggcacaa caccacgtct
      781 tccgcccggg caatggcatc ggcaggccag gtggtgagca cttcctgctc tatcttcatc
      841 acggcctcat tcattgacgg tgactgcgtt ttctgtggat ggttatgcca cagggacatc
      901 gccaccag
//
LOCUS       DAJTNA010000419          902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTNA010000419 DAJTNA010000000
VERSION     DAJTNA010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.426"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            7..558
                     /gene="ybcL"
                     /locus_tag="OO161_004378"
     CDS             7..558
                     /gene="ybcL"
                     /locus_tag="OO161_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5465152.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            568..>902
                     /locus_tag="OO161_004379"
     CDS             568..>902
                     /locus_tag="OO161_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5465153.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQ"
BASE COUNT          276 a          170 c          198 g          258 t
ORIGIN      
        1 gtttttatga aaaaacttat cgtttcaagt gtattggcat tcataacatt ttctgcgcag
       61 gccgcagcat ttcaggtcac tagtaatgaa ataaaaacag gagagcaact tacaacgtct
      121 catgtatttt ctggatttgg gtgtgagggg ggtaatacat cgccctcatt aacctggtct
      181 ggtgctccag aaggcaccaa aagctttgcc gtaactgtgt atgatccaga tgcgcctaca
      241 ggcagcggat ggtggcactg gactgttgct aatattccag caacggtaac atatttgccc
      301 gctgatgcag gaagacgtga tggtacaaaa cttccgactg gtgctgtcca aggccgaaat
      361 gattttggct atgcgggatt tggtggagca tgtcctccca aaggagataa accccatcat
      421 taccagttta aaatatgggc tctaaaaact gataagattc ctgtggattc taattccagc
      481 ggagcgttag ttggttatat gcttaatgcc aataaaattg caaccgctga gataataccc
      541 gtttatgagg taaagtagga taagagtatg ctggcaaaag ataggactaa cctaaagatt
      601 gaagaaatac ggatgcataa acatcatgag attcacaggg ttaagcctct tatgccagct
      661 ttatgtcgta tccgtcaggg aaaaaaagtt atcaattggg agacgcatag tctaactgtt
      721 gataataatc agataatatt atttccctgt ggatatgaat tttatattgc gaattaccca
      781 gaagcaggtc tttatcttgc agaaatgctt tattatccca ttgatctaat tgaaaagttc
      841 caaaatcttt atgcgataac cgatcaaatt cgtaacacga caggtttctg cttacctcag
      901 aa
//
LOCUS       DAJTNA010000420          902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTNA010000420 DAJTNA010000000
VERSION     DAJTNA010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.427"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..695)
                     /locus_tag="OO161_004380"
     CDS             complement(<1..695)
                     /locus_tag="OO161_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5465154.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLNDINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          268 a          144 c          190 g          300 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac attaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagctct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatatca ttaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gagggggatt atttacagga agcataataa cctcgcagaa ggatatcctg
      721 ataaaatgtg atactgggaa ataataagct aaaataaaat gtttcaggat tgaaatttat
      781 tgttttgatt ttaaggaagt gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 ca
//
LOCUS       DAJTNA010000421          895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTNA010000421 DAJTNA010000000
VERSION     DAJTNA010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.428"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..666)
                     /gene="ipaH9.8"
                     /locus_tag="OO161_004381"
     CDS             complement(<1..666)
                     /gene="ipaH9.8"
                     /locus_tag="OO161_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5465155.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQ"
BASE COUNT          266 a          142 c          203 g          284 t
ORIGIN      
        1 ttgcagagca tgggatgata atgggttatc actaatatgt attgaacatt cattcctcag
       61 attaagaatg ctttccggga tatgacttat ctgatttcta tcaaaaaaat actctctgac
      121 aacgggatta tttccctcag aaaatgctgg tagctcagtg aggaaattac gggtcgctct
      181 gagattttta agagcctgtg ggagcgatgg taaggagact atttcgttat agctgatatt
      241 catagtcaat aacgaatcgg gtaaagctgg caggttttcc agctcattgt gttgtacctg
      301 aagtgactcc agcgcaggag gtagcacggg caattctgat aatttattgc tggcggaata
      361 taattttttt agcgtaacag gcagttcagg taggttagtt aattgattat atgatacatt
      421 gagcagcgtt atctgagctg gtaagttgtc aggtagcgag gacagattta aacggtccag
      481 tcgaagttca tcggaattat tgataagaca ttctttaagt cgggagacag cctcatcacg
      541 ctcttcaccg gggagcgctt gtttttccca tttatcccat gctgaaaagt aatcagcata
      601 tgtaccggaa atagtgttat aaaaagaatt ttggggcaat gaaaagttat tatttatcgg
      661 taacatgatg ggcatccagt taaagccggt ggacaaatgg ctttgttaaa agaaataagc
      721 aggagttttt atgaagttca ttccatgaaa tgaacttcag cactttttta ttataataag
      781 gataaacaaa attggctgta aaaaaacgat actgtttcag ttaaccgtga atggcctgtt
      841 tgcaccgtat tgttcttgtt tttcggttag ttaaacatcg tacttcatat ttgaa
//
LOCUS       DAJTNA010000422          863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTNA010000422 DAJTNA010000000
VERSION     DAJTNA010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.429"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..426
                     /locus_tag="OO161_004382"
     CDS             <1..426
                     /locus_tag="OO161_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5465156.1"
                     /translation="GAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREK
                     LTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNN
                     EHIQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK"
     gene            656..>863
                     /locus_tag="OO161_004383"
     CDS             656..>863
                     /locus_tag="OO161_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5465157.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSI"
BASE COUNT          203 a          208 c          252 g          200 t
ORIGIN      
        1 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
       61 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
      121 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
      181 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
      241 gtgcgcttcg gtgaagagga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
      301 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
      361 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgtt ggcaggtcgt
      421 aagtaatcca atcccaccct ctccacatgg agaaggtggg gttaattgcc tgatgcgcta
      481 cgcttatcag gcctacagga tgcatcacga tttgttgaat ttgcacgttc ttgtaggccg
      541 gataaggcgc ttacgccgca tccggcgctg cccctctcct cacggagagg gtttgggtga
      601 gggaaaagcc tcaccccagc cctctcgggt aaaaacattg atgaaggtta atactatgaa
      661 agcattacat tttggcgcag gtaatatcgg tcgtggcttt atcggtaaac tgctggcaga
      721 cgcgggtatc caactgacgt ttgccgatgt caatcaggtg gtacttgatg ccctgaatgc
      781 ccgtcatagc tatcaggttc atgtggttgg tgaaaccgag caggtagata ccgtttccgg
      841 cgtcaatgct gtcagcagca ttg
//
LOCUS       DAJTNA010000423          857 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTNA010000423 DAJTNA010000000
VERSION     DAJTNA010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.430"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>857
                     /gene="tuf"
                     /locus_tag="OO161_004384"
     CDS             <1..>857
                     /gene="tuf"
                     /locus_tag="OO161_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5465158.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTL"
BASE COUNT          202 a          212 c          245 g          198 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccct
//
LOCUS       DAJTNA010000424          849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTNA010000424 DAJTNA010000000
VERSION     DAJTNA010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.431"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..801)
                     /gene="wecH"
                     /locus_tag="OO161_004385"
     CDS             complement(<1..801)
                     /gene="wecH"
                     /locus_tag="OO161_004385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5465159.1"
                     /translation="MVVMIHTTTWYVTNAHSVSPVTWDIANVLNSASRVSVPLFFMIS
                     GYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINMELALKNLLQKPVFYHLW
                     FFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVPQKIDGFEWLPINLYIN
                     GDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIISRGTLYELQWRGNFADT
                     WYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGIYGFHALI"
BASE COUNT          263 a          208 c          194 g          184 t
ORIGIN      
        1 aatcaaggcg tggaatccgt atatacccaa tgaatggcgg gagattaagc caagtccgcg
       61 aatggtacgc gtatccagcg tgtttttaac cagagtcaat agcgcgattg cgcagataaa
      121 aaccatcggc ccacagtaaa gataccaggt atcggcaaaa tttccgcgcc actgcaattc
      181 atataatgtc ccgcgagaga taataaaaac ccccgtcgca aacagcgcgg cgctcaccca
      241 cgacagtgct ttatgctgtg tgtccatcat ccctatagcg cggcccaaca tgccatacag
      301 aatgtagtaa aaagtatcgc cattgatata taagttaatt ggcagccatt caaaaccgtc
      361 aattttctgc ggcactgtgt ttgggttagc gataatgcca atcaccgcca ttagcaccag
      421 caacattttt ccgccgacgt tcttcacctg aatcagcggt gaaaccagat aaatcaccgc
      481 aatcgcgaag aaaaaccaca agtggtaaaa cactggcttt tgcagcaggt ttttcagcgc
      541 taactccata ttgatggagg taaacagcgc aatgtagagc agtgcgattg cgctataaaa
      601 aatcagacat aagccgatac gcaagaaatg gcgcggctgg gcgctgcgtt cgccaaaaaa
      661 gagatagccg gaaatcatga aaaatagcgg cacgctgaca cgagaggcag aattcagaac
      721 attggcgata tcccatgtga cggggctaac actatgagca ttggtcacat accaggtagt
      781 ggtgtgaatc atcaccacca ttaaacacgc tatccctcgc aggttatcaa tccagtaaat
      841 tttgggctg
//
LOCUS       DAJTNA010000425          843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTNA010000425 DAJTNA010000000
VERSION     DAJTNA010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.432"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(32..214)
                     /locus_tag="OO161_004386"
     CDS             complement(32..214)
                     /locus_tag="OO161_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465160.1"
                     /translation="MYNCGESYSEYDISLSDDENYNDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
     gene            545..>843
                     /locus_tag="OO161_004387"
     CDS             545..>843
                     /locus_tag="OO161_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR5465161.1"
                     /translation="MNPSDAIEAIEKPLSALPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMIITDLPGVGESRD"
BASE COUNT          228 a          205 c          168 g          242 t
ORIGIN      
        1 attgattgtc ttattatcag ctaccctcgt attaatcaga gttttcattt atttttagtg
       61 cataatcaga tgccgcctca tcattatcct cagtattatc atagccgaaa acttctcccg
      121 ggcacgggcg ttcacgataa tagtcatcgt tatagttttc atcgtcagat aatgatatgt
      181 cgtattctga atatgattca ccacaattat acataaaatc atctcctttg ggatggttac
      241 aataattagt gagtttattc gaaaaaataa tgagcgccag accaatccac ccgacttttg
      301 attataatca ttaagatgcc tgtctcagta caaatagaat caaactcaat acagcatgat
      361 tcacctttat attctctcag ggaaaccaaa taccactatt caactattaa gaataaaatt
      421 ctggaggtgc gattatctga agatacctgc atcctaaata atttcagaca gatattatcg
      481 ttgtgaatcc cccgccacct ttccggtgcg cgggattttg tcttttttca ccgggaatac
      541 atgtatgaat ccgtctgatg ccattgaggc aattgaaaaa ccgctttccg ccctgcctta
      601 ctcactttcc cgtcacatcc tggaacatct gcgcaaactc acccgtcacg aacccgtgat
      661 tggcattatg gggaaaagcg gggccggtaa atcctcactc tgtaatgccc tgtttcaggg
      721 ggaagtcacc ccggtcagtg atgttcacgc cggcacccgg gaagtgcggc gcttccgtct
      781 gagtggccac ggtcacagca tgattatcac tgacctgccc ggggtgggcg agagccggga
      841 cag
//
LOCUS       DAJTNA010000426          842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTNA010000426 DAJTNA010000000
VERSION     DAJTNA010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.433"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            34..842
                     /locus_tag="OO161_004388"
                     /pseudo
     CDS             34..842
                     /locus_tag="OO161_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          194 a          213 c          218 g          217 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcagtt tactggtggg ctacatcgcc
       61 tggctggcga ccgcttaccg gatgagcttt tcccgcgaaa tcaatctggg cctggcgcaa
      121 catgagttcg aattgttctg tcagcctttg cttaatgcgc gcagccagca atgtattggt
      181 gtagagattt tgctgcgctg gaacaatccg cgtcagggcc ggatttcacc ggatgtgttt
      241 attcctatcg cggaagaaca tcatttaatt gtgccactga cccgctatgt gatggcagaa
      301 accattcgtc agcgccatgt tttcccgatg agtagtcagt ttcatgttgg cattaacgtc
      361 gcacccagcc attttcgccg tggtgtgctg ataaaagatc tcaatcagta ctggtttagc
      421 gctcacccga ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt actggatgtt
      481 gattatcgga ttgcccgcga gctacatcgt aaaaacgtca aactggcgat tgatgacttc
      541 ggcaccggca acagttcatt ttcctggctt gaaacattac gtcctgacgt gctgaaaatt
      601 gataagtcat ttaccgcagc tataggttct gacgcggtta actcgacggt gaccgatatc
      661 atcatcgcgc tggggcaaag actgaatatt gaactggtgg cggagggcgt ggaaacgcaa
      721 gaacaggcga agtatttgcg ccgtcatggc gtgcatattt tgcaagggta tttgtacgca
      781 cagccgatgc cgctacgtga ttttcccaaa tggctggcgg gcagccaacc gccgcccgcc
      841 cg
//
LOCUS       DAJTNA010000427          842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTNA010000427 DAJTNA010000000
VERSION     DAJTNA010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.434"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..424
                     /gene="hipA"
                     /locus_tag="OO161_004389"
     CDS             <1..424
                     /gene="hipA"
                     /locus_tag="OO161_004389"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCR5465162.1"
                     /translation="FQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDIISAFPVLGGTG
                     IHISDLKLAMGLNASKGKKMAIDKIYPRHFLATATVLRFPEVQMHEILSDFARMIPAA
                     LDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            684..776
                     /locus_tag="OO161_004390"
                     /pseudo
     CDS             684..776
                     /locus_tag="OO161_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT          217 a          182 c          217 g          226 t
ORIGIN      
        1 cttccagtgg ttgattggcg caacggatgg ccatgcaaaa aacttctccg tatttattca
       61 ggctggcggc agttatcgac tcacgccatt ttacgacatc atttcagcat ttccggtcct
      121 tggcggtacg ggaatacaca tcagcgatct caaactggca atggggctta acgcatccaa
      181 aggcaaaaaa atggcaatcg ataaaattta tccgcgacat tttttggcga cagcaacggt
      241 gctgagattc ccggaagtgc agatgcatga aatcctaagt gactttgcca gaatgattcc
      301 ggcagcactg gataacgtga agacttcatt accgacagat tttccggaga acgtggtgac
      361 ggcagttgaa accaatgtgt tgaggttgca tggacggtta agccgagaat acggtagtaa
      421 gtgagatatg gggtttggtc gttgttaata agcaaccatg accgccaacg actggattct
      481 cccttggttt atgttggtgc gcataatgta tattatgtta aatcatatct gagtgatgcg
      541 aagtgctttt ctggtagtcg ttattcgttc agggttccag aacttaagcc tctcctcttt
      601 cataattggc ctgtgcatga cggatatagg ctggctggta tgcagatttg ccgtttgccg
      661 catcttccca gcgttgaaga ctcaagctgg caaaccgcgt gcaaatgagc acgccctctt
      721 tccatcaaca cttcaggcag cttgcgggaa tgagtccact acggtatcag aaatgggaag
      781 tgggtgtctc tctgatgcgt aatggaaaaa tactggctac gggagaaaat gtctccctag
      841 gg
//
LOCUS       DAJTNA010000428          810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTNA010000428 DAJTNA010000000
VERSION     DAJTNA010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.435"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>810
                     /gene="dsdA"
                     /locus_tag="OO161_004391"
     CDS             <1..>810
                     /gene="dsdA"
                     /locus_tag="OO161_004391"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5465163.1"
                     /translation="GTTSLAEGLPYVGLTEQDVQDAHARLSRFAPYLAKAFAETAATG
                     GIIESELVAIPAMQKRLEKEYQQPISGQLLLKKDSHLPISSSIKARGGIYEVLAHAEK
                     LALEAGLLTLDDDYSKLLSPEFKQFFSQYSIAVGSTGNLGLSIGIMSARIGFKVTVHM
                     SADARAWKKAKLRSHGVTVVEYEQDYGVAVEEGRKAAQSDPNCFFIDDENSRTLFLGY
                     SVAGQRLKAQFAQQGRIVDADNPLFVYLPCGVGGGPGGVAFGLKLAFGDHV"
BASE COUNT          191 a          192 c          219 g          208 t
ORIGIN      
        1 ctggcacgac ctcattggct gaaggtttac cttatgttgg cctgaccgaa caggatgttc
       61 aggacgccca tgcgcgctta tcccgttttg caccctatct ggcaaaagca tttgctgaaa
      121 ctgctgccac tggggggatt attgaatcag aactggttgc cattccagct atgcaaaaac
      181 ggctggaaaa agaatatcag caaccgatca gcgggcaact gttactgaaa aaagatagcc
      241 atttgcccat ttccagctcc ataaaagcac gcggcgggat ttatgaagtc ctggcacacg
      301 cagaaaaact ggctctggaa gcggggttgc tgacgcttga tgatgactac agcaaactgc
      361 tttctccgga gtttaaacag ttctttagcc aatacagcat tgctgtgggc tcaaccggaa
      421 atctggggtt atcaatcggc attatgagcg cccgcattgg ctttaaggtg acagttcata
      481 tgtctgctga tgcccgggca tggaaaaaag cgaaactgcg cagccatggc gttacggtcg
      541 tggaatatga gcaagattat ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg
      601 acccgaactg tttctttatt gatgacgaaa attcccgcac gttgttcctt gggtattccg
      661 tcgctggcca gcgtcttaaa gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata
      721 accctctgtt tgtctatctg ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg
      781 ggcttaaact ggcgtttggc gatcatgttc
//
LOCUS       DAJTNA010000429          787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTNA010000429 DAJTNA010000000
VERSION     DAJTNA010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.436"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..574
                     /locus_tag="OO161_004392"
                     /pseudo
     CDS             1..574
                     /locus_tag="OO161_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT          268 a          155 c          153 g          211 t
ORIGIN      
        1 agataaacaa accattaaac aaactttatt tgcccgtcag ccagcaaaaa tccacaaacc
       61 ctatccaact aatagcaaga ttattatggg agatgtactg aatcatgcag cactgaagca
      121 ggccatgcag ggacaggacg ttgtgtatgc caaccttacg ggggaagatt tagatattca
      181 ggcaaattca gtgatcgctg cgatgaaagc ctgtgatgtt aaacgtctga tctttgttct
      241 ttccctcggt atttatgatg aagttccagg aaaatttggc gaatggaata atgcagttat
      301 tggtgagccc ctaaaaccat tccgcagggc tgcggatgca attgaggctt ctggactgga
      361 atatacaatt cttcgccctg cctagctgac tgatgaagat attattgatt atgagcttac
      421 ctcacgcaat gaaccattca aaggtacgat cgtatcacgt aagagtgtcg cagcattaat
      481 cacagatatc atcgataaac ctgagaaaca cattggtgag aatataggta tcaatcaacc
      541 cgtgactgat ggtgacaagc cattattcat gtaaatcaat tacatagtta ccatcgtaat
      601 gtactatgtg cagtacaatt tattaaactt attaaggtga actgatcaat ccgatgcgat
      661 taacatgtgt agaaccagta agaataaaat acgccatgta tataacatta cgacttacat
      721 ctaagtcagc ataattctaa tatctgcaca tcaataaaaa tgcaaacaac ttccggcaaa
      781 aaaagtg
//
LOCUS       DAJTNA010000430          776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTNA010000430 DAJTNA010000000
VERSION     DAJTNA010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.437"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(47..383)
                     /locus_tag="OO161_004393"
                     /pseudo
     CDS             complement(47..383)
                     /locus_tag="OO161_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          250 a          142 c          141 g          243 t
ORIGIN      
        1 taaatatgaa aagatggttt aaataccccg ttacttttga cttacactat acggtatcgc
       61 atcgtttaat attcgcaccg gccagatttt tatttctatt agttgtcaca atactgaatg
      121 cgtacgacca cagtattctg gctcctgtgt ggtcatgctt taattctgcg ttccggacag
      181 ataagcagtt gcttgcagga atccttcttg tgttaatgtc agttcccctt tttaccagtg
      241 ctgatttcca cattccgtcc aacagagctt atagcctttc cctggattat agcattgtcc
      301 ggctgaagtt ctttttgaat aataatagaa gcactgctgg cagatccagt ccgtttttca
      361 taacccactg tactgataac cataatctaa tcagtagaaa ttgagtcgaa aataagcact
      421 actccataca ggataattag aggtcagttt gattattcac aattcatcat cagcattttc
      481 tgtttctgac gaaatcaaca tgaaaagaac catatatgat aattattata ataacggttt
      541 taattggaat acatatatta caacgtatta tatataattg gtattctagg aactatattc
      601 tcaaaataca gtagaaacga ggtatgtttc tggtggaaag gacagtggga ttaaaaagta
      661 agaattgata aaaaaacgcc agcaacacat agtgccggcg gagggaatac cccatggaga
      721 aaatgtgatg cctagagcac catgaatata taattaaaaa tagttagcgt tgtcaa
//
LOCUS       DAJTNA010000431          763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTNA010000431 DAJTNA010000000
VERSION     DAJTNA010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.438"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(16..720)
                     /locus_tag="OO161_004394"
     CDS             complement(16..720)
                     /locus_tag="OO161_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HCR5465164.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEIRLVSRVFEM"
BASE COUNT          175 a          205 c          195 g          188 t
ORIGIN      
        1 tcttattcaa aggccttaca tttcaaaaac tctgcttacc aggcgtattt cgcccagggg
       61 atcaccataa taaaatgctg aggcctggcc tttgcgtagt gcacgcatca cctcaatacc
      121 tttgatggtg gcgtaagccg tcttcatgga tttaaatccc agcgtggcgc cgattatccg
      181 tttcagtttg ccatgatcgc attcaatcac gttgttccgg tacttaatct gtcggtgttc
      241 aacgtcagac gggcaccggc cttcgcgttt gagcagagca agcgcgcgac cataggcggg
      301 cgctttatcc gtgttgatga atcgcgggat ctgccacttc ttcacgttgt tgaggatttt
      361 acccagaaac cggtatgcag ctttgctgtt acgacgggag gagagataaa aatcgacagt
      421 gcggccccgg ctgtcgacgg cccggtacag atacgcccag cggccattga ccttcacgta
      481 ggtttcatcc atgtgccacg ggcaaagatc ggaagggtta cgccagtacc agcgcagccg
      541 tttttccatt tcaggcgcat aacgctgaac ccagcggtaa atcgtggagt gatcgacatt
      601 cactccgcgt tcagccagca tctcctgcag ctcacggtaa ctgatgccgt atttgcagta
      661 ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga atgggttcat
      721 gtgcagctcc atcagcaaaa ggggatgata agtttatcac cac
//
LOCUS       DAJTNA010000432          749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTNA010000432 DAJTNA010000000
VERSION     DAJTNA010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.439"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            37..>749
                     /locus_tag="OO161_004395"
     CDS             37..>749
                     /locus_tag="OO161_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5465165.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKCAGVAVVLSLAA
                     VTSVPALAADTVVQAGETVSGGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTASNTTISSGGLQFVGAGGKATDTIINEGGGQSLKGLALNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGAVATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINK
                     NGRQIVAAEGTANTTVVYAG"
BASE COUNT          197 a          181 c          233 g          138 t
ORIGIN      
        1 ccggcttttt tattcaccct ctgtaaggaa aagctgatga aacgacatct gaacaccagc
       61 tacaggctgg tatggaatca cattacgggc accctggtgg tggcttccga actggcccgc
      121 tcacggggaa aatgcgccgg tgtggcggtt gtgctgtctc ttgctgctgt cacatcagtc
      181 ccggcactgg ctgctgacac ggtcgtacag gcgggagaaa ccgtgagcgg cggaacactg
      241 acaaatcatg acaaccagat tgttctcggt acggccaacg gaatgaccat cagtaccggg
      301 ctggagtatg ggccggataa cgaggccaat accggcgggc aatgggtaca ggatggcggc
      361 acagccagta ataccaccat cagcagcggc gggctccagt ttgtaggtgc cgggggaaag
      421 gctacagaca caataatcaa tgaaggaggc ggacaaagtc tgaagggact ggcactgaac
      481 accacgctga atggtggcga gcagtggatg catgaaggtg cgatagccac aggaaccgtc
      541 attaatgata agggctggca ggtcgtcaaa cccggcgcag tggcaacaga caccgttgtg
      601 aataccggcg cagaaggagg accggatgca gaaaatggtg ataccgggca gtttgttcgc
      661 ggaaatgccg tacgtaccac catcaataaa aatggtcgtc agattgtggc tgctgaagga
      721 acagcaaata ccactgtggt ttatgccgg
//
LOCUS       DAJTNA010000433          727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTNA010000433 DAJTNA010000000
VERSION     DAJTNA010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.440"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
BASE COUNT          285 a          136 c           80 g          226 t
ORIGIN      
        1 aaaaagttaa gaattaacat gggtaggccg tttaaagagc ctacccactg acttatttat
       61 ctttttttga aaaaaattaa gataagaaaa agaacaatat ttgttacagg aaataccccc
      121 aaaactcccc agtacttact ccatcctaga gacatcgtat atctaccaag gatttgacaa
      181 aacccaaaac ttaaccctat acctatactt cttaagttta atgtcgtagt caggcttaaa
      241 ataaatagaa caaggatggt aatagcccac cacaataacc atgccttaac aaaatcagat
      301 tttttgctgt aaggttattt acccaagaag aactgccaaa agttaccatt tcccatatag
      361 aatccattca cccaagatat tgtttataga ataaaaatac atttcaatta tatttaatta
      421 aaatataccc catcaataaa aacttacaca ctctaaatta ctactgtata gttatcaatg
      481 caaagactgc tatattgcat atatatatgc aaaccacata attcaggtaa tatatattca
      541 aaacaattat atatataaaa caatgtaaaa atgttcaaat catccatcag aaatatattc
      601 tacccatcca ggcttgagcg tttatcacgc tttcagttca taatctatac attaacatca
      661 tggtcaatat taataacttt gaatgatatc aacctggctt tgagttttat tctggttatt
      721 aataata
//
LOCUS       DAJTNA010000434          710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTNA010000434 DAJTNA010000000
VERSION     DAJTNA010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.441"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..217
                     /locus_tag="OO161_004396"
     CDS             <1..217
                     /locus_tag="OO161_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465166.1"
                     /translation="AEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRET
                     EQQIYRQLTDEVLALRLPENGSQLHHS"
     gene            complement(218..397)
                     /locus_tag="OO161_004397"
     CDS             complement(218..397)
                     /locus_tag="OO161_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465167.1"
                     /translation="MNKPQLAESQKTALLTEAESVIRPRGRAVRLNRETDESGEARGR
                     ASVFPESVRSVVYAM"
     gene            complement(397..>710)
                     /locus_tag="OO161_004398"
     CDS             complement(397..>710)
                     /locus_tag="OO161_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5465168.1"
                     /translation="EQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAW
                     SDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDV
                     E"
BASE COUNT          162 a          212 c          185 g          151 t
ORIGIN      
        1 ggcagaagag cagaaatatg agatgctgga gaatgagtac cctcagaggg tggctgaccg
       61 gctgaaagca tcaggtctga gcggtgatgc ggatgcggag agggaagccg gtgcacaggt
      121 gatgcgtgag actgaacagc agatttaccg tcagctgact gacgaggtac tggccctgcg
      181 attgcctgaa aacggctcac aactgcacca ttcataatca catcgcataa accacagacc
      241 ggactgactc cggaaaaaca gaggcccgcc cccgggcctc cccggattca tccgtttccc
      301 tgttcagcct gacagcacgc ccccgcggcc ggatgacaga ctccgcttcg gtaagcaaag
      361 cggtcttctg tgattccgcc agttgcggct tattcattac tcaacgtcaa acgcccgaat
      421 tgaagccaaa tcatccagac cgctcagctc ctctttcatt tcacgctgac ggcgataaat
      481 ctcatcgttg cgatcgacct gcgcctgcac cattgctgcc gccagttctt ccagttccgg
      541 catcgacagt ttcacctgct gattatcggc atcgctccac gccatatgtg tttgtgctgt
      601 gacagatttt gccagcatga ctaccgggga caggcggccc agtgagtcgg gaccagcatt
      661 ccagatacga ccgttccatt caaacgtgaa cggcttcgcc tcctgttctg
//
LOCUS       DAJTNA010000435          701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJTNA010000435 DAJTNA010000000
VERSION     DAJTNA010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.442"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            90..508
                     /locus_tag="OO161_004399"
                     /pseudo
     CDS             90..508
                     /locus_tag="OO161_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            582..>701
                     /locus_tag="OO161_004400"
     CDS             582..>701
                     /locus_tag="OO161_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5465169.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMT"
BASE COUNT          199 a          156 c          175 g          171 t
ORIGIN      
        1 cctggtctac tgcctgttgt gacagcgcag gcgctgcgct tatgtactgc acccattttg
       61 ttggacgatg aaatggaata gcccctaata tgtcaaagcc aaaataccct tttgaaaagc
      121 gccttgaagt cgtgaatcac tacttcacta ctgatgatgg ttacaggatc atctcggcac
      181 gttttggtgt ccccgaaccc aggtcaggac atgggttgcc ctctatgaaa aacatggaga
      241 aaaaggctta attcccaaac ctaaaggtgt tagtgctgat ccagagttgc gtatcaaggt
      301 cgtgaaagct gtgattgagc agcacatgtc ccttaatcag gctgctgctc actttatgct
      361 tgctggtagt ggttctgtag ccaggtggct gaaggtttat gaagagcgcg gagaagctgg
      421 tttacgcgcg ctcaagattg gcaccaaaag aaacattgca atatcagttg atccagaaaa
      481 agcggcatca gcattggagc tgtcaaaagt gaagtggcac actgaatttg gccacctgaa
      541 cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa agaaatttta
      601 gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac acggtggcag
      661 atgccgccaa agctatggat gttggccttt ccacaatgac a
//
LOCUS       DAJTNA010000436          698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTNA010000436 DAJTNA010000000
VERSION     DAJTNA010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.443"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>698
                     /locus_tag="OO161_004401"
     CDS             <1..>698
                     /locus_tag="OO161_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HCR5465170.1"
                     /translation="ALTAHLDKLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKIIQPD
                     TDHCPECDEPLHYIRDAVSEKLEYIPAHFVVNRHVRPQYSCPCCQKVFSGEMPAHILP
                     KSAVEPSVIAQVIINKYGDHLPLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAA
                     LLHRELINRPVVHADETTLKILNTKKGGKSCSGYQWAYVSGERTGPSVVCFDCRTGRS
                     HEYPENWLQGWGGTL"
BASE COUNT          159 a          194 c          192 g          153 t
ORIGIN      
        1 gcgcttaccg cacatctgga taaactgctc ccgcaatccc ctgaagaaga tgaaaaagcg
       61 tcccgttcac gcccgatacg caaaccctta ccggttcatc ttccacgggt ggaaaaaatt
      121 atccagccgg acactgacca ttgccctgaa tgtgacgagc cgctgcacta tatccgcgat
      181 gcggtgagtg aaaagctgga gtatattccc gctcactttg tggtgaaccg tcatgtccgt
      241 ccgcaataca gttgtccctg ttgccagaag gtgttcagcg gtgaaatgcc ggcacatatc
      301 ctcccgaaaa gtgccgttga gccatcagtc atcgcacagg tgatcatcaa taaatacggt
      361 gaccacctgc ctctgtatcg ccagcaacag gtctttgccc gttcagatgt cgggctgccc
      421 gtcagttcga tggctgacat ggttggcgcg gcgggtgccg cattatctcc cctggcggcg
      481 ttactccatc gcgagttgat aaaccgtccg gtggtgcatg cagatgagac taccctgaag
      541 atcctgaaca cgaagaaagg cggtaaatcc tgctccggtt atcagtgggc atacgtcagt
      601 ggagaaagga cgggaccgtc agttgtgtgc ttcgactgcc ggaccggacg tagccatgag
      661 tatcctgaaa actggcttca gggctggggc gggacgct
//
LOCUS       DAJTNA010000437          649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJTNA010000437 DAJTNA010000000
VERSION     DAJTNA010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.444"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..332)
                     /locus_tag="OO161_004402"
     CDS             complement(<1..332)
                     /locus_tag="OO161_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465171.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDV"
     gene            complement(414..>649)
                     /locus_tag="OO161_004403"
     CDS             complement(414..>649)
                     /locus_tag="OO161_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5465172.1"
                     /translation="NTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKA
                     KQTLNFKAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT          165 a          160 c          132 g          192 t
ORIGIN      
        1 acgtcttctt ttaaaccaaa ctgcgctacc agttccggtg tcagacaagc atagcttttg
       61 ctcgcgtcat cttcaccagc ataccagtaa atatcgtact cttccgccaa tggctgttta
      121 tttagttgaa cctgtaaatt gtattttccg ggctcaacat atccctggct ggaaaaacgc
      181 ttcagatcaa tttttgtgtc gccttttaat tccagaaaac gggaatcaaa ctgaatgtcg
      241 tcttcagccc agacactgct ataactccct gacattgcca gcgcaatgca ccacggaaga
      301 atccgtaatc gaaaaagaga atggtcaggc atacacgttt ctatccgttt ttattcttgt
      361 tcggtcaaaa tccatttaaa tcccccatag cagtgtcgct atgggggtat gccttacagg
      421 taagtaatct ggaaggtggt atttgcttcg aaattaccca gatctggcgc atcagctgcg
      481 ccgaccagcc aggctttaaa gttcagtgtc tgcttcgctt tgcctttatt agtagaagtg
      541 tctttcacta ttttctggtc aatacccatg ccgcttttgt aagaggtacc cagagagtca
      601 ccgatcgcca ggctaacatt gttaaatgcc gcaccggtat cggtattga
//
LOCUS       DAJTNA010000438          648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJTNA010000438 DAJTNA010000000
VERSION     DAJTNA010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.445"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..392
                     /locus_tag="OO161_004404"
                     /pseudo
     CDS             1..392
                     /locus_tag="OO161_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          170 a          169 c          164 g          145 t
ORIGIN      
        1 gacgtcatcg tctgcgcgga aatggacgaa cagtggagct acgtcggggc taaatcacgc
       61 caggctggct gttttacgcg tatgacaggg tacgcaggac tgtcgtggcc cacgtttttg
      121 gtgaacgcac aatgttcacg ctggagcgtc ttctgagcct gctgtcggcc tttgacgtgg
      181 tggtattgat gacggacgga tggccgcttt atgaatcccg cctgaaggaa aagctgcacg
      241 ttatcagcaa gcgatatacg cagcgaattg agcggcataa tctgaggcag catctggcaa
      301 ggccgggacg gaagtcactg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg
      361 ggcattatct aaacataaaa cactatcagt aagttggatt cattacccta cctacctctt
      421 ggcaggaaaa agcgctcgca aaaacggtga gaaaaaaaca agcctatatc agataacagc
      481 cctctcctag acagggctgt tactttgccg taaccacaga atctgtcata ctacactacc
      541 gcctaagtca gtatacactc cgctagcgct acgtgactgg tttagggctg cgtcccgaaa
      601 cccgctaacc acctctcagc tactggatat caccccatgc catctgaa
//
LOCUS       DAJTNA010000439          648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJTNA010000439 DAJTNA010000000
VERSION     DAJTNA010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.446"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            532..>648
                     /locus_tag="OO161_004405"
     CDS             532..>648
                     /locus_tag="OO161_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5465173.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGV"
BASE COUNT          202 a          183 c           94 g          169 t
ORIGIN      
        1 tcgaactcag gcttttttat tggcatttca tctccatctg cggcacacgc aattgccaaa
       61 gcatatcgat acagcacctt accctgcccc tcctgccaca ataacatcta ccagcgactc
      121 ttccagccaa caccccaaat gcacattctg atgaattacc ccgtaacaaa cacgcttccc
      181 aaatagaaat ctttgcaaaa atagtgcatg aaataaactt tttctctacc aatccgcgat
      241 ataaatattc atcagcacca caatattcca catcactaaa tctctcccca ccgccccgca
      301 tctctgataa tggtctaaaa tcattgaggc cacttgcgac gacgttgctc aataaggaaa
      361 atttggcgtg aataatattc aacaccagtg cagcatcatc attacatact tacacccgcc
      421 gactaccgct ttcgatatat tgactcaatt ttccagcctg gttaaaaata gcgaccagct
      481 cagtcgcaaa tatagtgact accctaacta agcaacaata aggaatacac tatgactgtt
      541 caaacaagta aaaatccgca ggtcgatatt gctgaagata atgcgttctt cccttcagaa
      601 tattcgctta gccaatatac cagtcccgtc tctgatcttg atggcgtg
//
LOCUS       DAJTNA010000440          647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJTNA010000440 DAJTNA010000000
VERSION     DAJTNA010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.447"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..75
                     /locus_tag="OO161_004406"
     CDS             <1..75
                     /locus_tag="OO161_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465174.1"
                     /translation="DGLRFAIREGGRTVGAGVVAKVLG"
     gene            258..413
                     /gene="bfd"
                     /locus_tag="OO161_004407"
     CDS             258..413
                     /gene="bfd"
                     /locus_tag="OO161_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5465175.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREGSA"
     gene            397..582
                     /locus_tag="OO161_004408"
                     /pseudo
     CDS             397..582
                     /locus_tag="OO161_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="iron storage protein; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
     gene            complement(579..>647)
                     /locus_tag="OO161_004409"
     CDS             complement(579..>647)
                     /locus_tag="OO161_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5465176.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          176 a          142 c          152 g          177 t
ORIGIN      
        1 gacggtctgc gtttcgcaat ccgtgaaggc ggccgtaccg ttggcgcggg cgttgttgct
       61 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
      121 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      181 ggcgcaaata aaactgtgct attgtaatca taaccattct catttacact ttgcgcagaa
      241 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      301 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      361 tgggaaatca atgcggtaag tgcattcgcg ccgcgcgcga aggatctgcg tgaggctatc
      421 gcatatgccg acagcgttca tgactatgtt agccgcgata tgatgatcga gatccttgtt
      481 gatgaagaag gtcatatcga ctggctcgaa actgaactgg atctcatcgg taaaattggc
      541 ctacaaaatt acctgcaatc acaaattaaa gttaaagatt aacggcctat cagcaaacta
      601 tcccagccta taacgaaacc cgcagccgcc agaaatggcc caaaagg
//
LOCUS       DAJTNA010000441          630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJTNA010000441 DAJTNA010000000
VERSION     DAJTNA010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.449"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>630
                     /locus_tag="OO161_004410"
     CDS             <1..>630
                     /locus_tag="OO161_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5465177.1"
                     /translation="SHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENR"
BASE COUNT          172 a          147 c          178 g          133 t
ORIGIN      
        1 agccatcaaa cttattgggg aacgagtaat gtcgatgagc aattccaggc tggattaaat
       61 actgcgttcg aagatatcaa ctggacgctc agctatagcc tgacgaaaaa cgcctggcaa
      121 aaaggacggg atcagatgtt agcgcttaac gtcaatattc ctttcagcca ctggctgcgt
      181 tctgacagta aatctcagtg gcgacatgcc agtgccagct acagcatgtc acacgatctc
      241 aacggtcgga tgaccaatct ggctggtgta tacggtacgt tgctggaaga caacaacctc
      301 agctatagcg tgcaaaccgg ctatgccggg ggaggcgatg gtaatagcgg aagtacaggc
      361 tacgccacgc tgaattatcg cggtggttac ggcaatgcca atatcggtta cagccatagc
      421 gatgatatta agcagctcta ttacggagtc agcggtgggg tactggctca tgccaatggc
      481 gtaacgctgg ggcagccgtt aaacgatacg gtggtacttg ttaaagcgcc tggcgcaaaa
      541 gatgcaaaag tcgaaaacca gacgggggtg cgtaccgact ggcgcggtta tgccgtgctg
      601 ccttatgcca ccgaatatcg ggaaaacaga
//
LOCUS       DAJTNA010000442          628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJTNA010000442 DAJTNA010000000
VERSION     DAJTNA010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.450"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            66..>628
                     /gene="istA"
                     /locus_tag="OO161_004411"
     CDS             66..>628
                     /gene="istA"
                     /locus_tag="OO161_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR5465178.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNM"
BASE COUNT          152 a          161 c          161 g          154 t
ORIGIN      
        1 gatccacacc caacgcctga aatcagatcc agggggtaat ctgctctcct gattcaggag
       61 agcttatggt cacttttgag acagttatgg aaattaaaat cctgcacaag cagggaatga
      121 gtagccgggc gattgccaga gaactggggc tctcccgcaa taccgttaaa cgttatttgc
      181 aggcaaaatc tgagccgcca aaatatacgc cgcgacctgc tgttgcttca ctcctggatg
      241 aataccggga ttatattcgt caacgcatcg ccgatgctca tccttacaaa atcccggcaa
      301 cggtaatcgc tcgcgagatc agagaccagg gatatcgtgg cggaatgacc attctcaggg
      361 cattcattcg ttctctctcg gttcctcagg agcaggagcc tgccgttcgg ttcgaaactg
      421 aacccggacg acagatgcag gttgactggg gcactatgcg taatggtcgc tcaccgcttc
      481 acgtgttcgt tgctgttccc ggatacagcc gaatgttgta catcgaattc actgacaata
      541 tgcgttatga cacgctggag acctgccatc gtaatgcgtt ccgcttcttt ggtggtgtgc
      601 cgcgcgaagt gttgtatgac aatatgaa
//
LOCUS       DAJTNA010000443          615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJTNA010000443 DAJTNA010000000
VERSION     DAJTNA010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.451"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>615
                     /locus_tag="OO161_004412"
     CDS             <1..>615
                     /locus_tag="OO161_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5465179.1"
                     /translation="GGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKEG
                     GLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVTGSNRLGNFTVENGKAD
                     GVVLESGGRLDVLEGHSAWKTLVDDGGTLAVSAGGKATSVTITSGGALIADSGATVEG
                     TNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGT"
BASE COUNT          143 a          157 c          202 g          113 t
ORIGIN      
        1 cggcggcgac cagacggtac acgggcatgc gctggatacc acactgaatg gcggttacca
       61 gtatgtgcac aacggaggca cagcctctga cacggttgta aacagtgacg gctggcagat
      121 tgtcaaggaa ggtggtctgg cggatttcac caccgttaac cagaaaggca aactgcaggt
      181 gaacgccggt ggtacagcca cgaatgtcac cctgaagcag ggaggcgcac tggtcaccag
      241 tacggcggca accgtcaccg gcagcaaccg tctgggcaat ttcactgtgg aaaacggtaa
      301 ggctgacggt gttgttctgg agtccggtgg tcgcctggat gtactggagg gccattcagc
      361 ctggaaaaca ctggtggatg acggcggaac cctggcagtg tctgccggtg gtaaggcgac
      421 aagtgtcacc ataacatccg gtggtgccct gattgcagac agtggtgcca ctgttgaggg
      481 gaccaatgcc agcggtaagt tcagtattga tggcacatcc ggtcaggcca gcggcctgct
      541 gctggaaaat ggcggcagct ttacggttaa tgccggagga ctggccagca acaccactgt
      601 cggacatcgt ggaac
//
LOCUS       DAJTNA010000444          602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJTNA010000444 DAJTNA010000000
VERSION     DAJTNA010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.452"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..588
                     /locus_tag="OO161_004413"
     CDS             <1..588
                     /locus_tag="OO161_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HCR5465180.1"
                     /translation="TITSGGNPPAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINK
                     NCRVLGKLSANQIEGDLVKTVGKPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPA
                     VAFRGAKHERKNNNIYSSCRLIVKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLE
                     FSVSAWLVNGWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          159 a          141 c          161 g          141 t
ORIGIN      
        1 accataacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
       61 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
      121 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
      181 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
      241 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
      301 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
      361 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
      421 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
      481 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
      541 ctggttgttg tgatgaagaa agccactgca ggcatcacga ttagctgaat tttataaccc
      601 ag
//
LOCUS       DAJTNA010000445          594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJTNA010000445 DAJTNA010000000
VERSION     DAJTNA010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.454"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..65)
                     /locus_tag="OO161_004414"
     CDS             complement(<1..65)
                     /locus_tag="OO161_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein E"
                     /protein_id="HCR5465181.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRAL"
     gene            complement(123..470)
                     /locus_tag="OO161_004415"
     CDS             complement(123..470)
                     /locus_tag="OO161_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5465182.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     GATGKLVVWDGQKAGSAVGILVLPLEGTEAVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGTALSHAALP"
BASE COUNT          123 a          176 c          149 g          146 t
ORIGIN      
        1 agcgcacgga atttaacttt ttgttcggta taaccgagta actggcgggt cgtaaacaat
       61 cccataaatc agttcctttc attaagaaat cagtcaggcc aacgcggtgg cctgataacg
      121 tgttacggca gcgccgcgtg actcagggcg gtgccggcaa aggcatttgc ctttttgtgt
      181 tcatccacac tgtcaggcca gcggattgcc tccgtcgcaa aggtccccga cttgtaatac
      241 gtcagcaccg cctctgtgcc ttcaagcggc agtaccagta tgccaaccgc actaccggct
      301 ttctgtccat cccagaccac cagtttcccg gtggccccat ccagcatcag gggtgtcagt
      361 gccggtgttg ccgaggaaat cccgctgctg cctgtggcgg tgtgagccgg atcattaccg
      421 gcaaaaatac gtacttccgc acgctgttca gtgatggttt tcgtcaccat attgttaaaa
      481 cctcgtattg atgttcagca ctgacttcat ggcatggcca tgagcatttt cacgtccgca
      541 tcaccgtctt ctgacgcctg tgacacgctc cccctggacc gcagccggtg aatg
//
LOCUS       DAJTNA010000446          593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJTNA010000446 DAJTNA010000000
VERSION     DAJTNA010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.455"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..65)
                     /locus_tag="OO161_004416"
     CDS             complement(<1..65)
                     /locus_tag="OO161_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein E"
                     /protein_id="HCR5465183.1"
                     /translation="MGLFTTRQLLSYTEQKVKFRAL"
     gene            complement(123..470)
                     /locus_tag="OO161_004417"
     CDS             complement(123..470)
                     /locus_tag="OO161_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5465184.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGTALSHAALP"
     gene            complement(507..>593)
                     /locus_tag="OO161_004418"
     CDS             complement(507..>593)
                     /locus_tag="OO161_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5465185.1"
                     /translation="HSPAAVRGGVSQTSADGDADVKMLMAMP"
BASE COUNT          128 a          171 c          146 g          148 t
ORIGIN      
        1 agcgcacgga atttaacttt ttgttcggta taactgagta actggcgggt cgtaaacaat
       61 cccataaatc agttcctttc attcagaaat cagtcaggcc accatggtgg cctgataacg
      121 tgttacggca gagccgcgtg actcagggcg gtgccggcaa aggcatttgc ctttttgtgt
      181 tcatccacac tgtcaggcca gcggattgcc tccgtcgcaa aggtccccga cttgtaatag
      241 gtcagcaccg tctctgtgcc ttcaagcggc agtaccagta tgccaaccgc actaccggct
      301 ttctgtccat cccagaccac cagtttcccg gtggcttcat ccagcatcag gggcgtcaga
      361 gccggtgttg cagaagaaat cccgctgctg cctgtggcgg tatgagccgg atcattacca
      421 gcaaaaatac gtacttccgc acgctgttca gtgatggttt tcgtcaccat tttgttaaaa
      481 cctcatattg atggtcagca ctgacttcat ggcatggcca tgagcatttt cacgtccgca
      541 tcaccgtctg ctgacgtctg tgacacgcca ccccgcaccg ctgccggtga atg
//
LOCUS       DAJTNA010000447          588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJTNA010000447 DAJTNA010000000
VERSION     DAJTNA010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.456"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..466
                     /locus_tag="OO161_004419"
     CDS             <1..466
                     /locus_tag="OO161_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5465186.1"
                     /translation="PELIYCWEQLKTSIISRGFSTQIQEHLAMGVLLSLGAHHVNCLL
                     LSDSKQSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDV
                     RLNNALSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          192 a          111 c          119 g          166 t
ORIGIN      
        1 tcccgagtta atatattgtt gggagcaact aaaaacatct attatttccc gaggcttctc
       61 aacgcaaatt caggaacact tagcaatggg cgttctactt tcattaggag cacatcatgt
      121 taattgttta cttttatcag atagtaaaca atcattaata agtcgttgtt ataacctaat
      181 gctatccgaa cctggaacaa aatggacagc aaacaaggta gcgagatatc tctacatttc
      241 tgtttccaca ttgcatcgcc gtctggcaag cgagggagta agttttcaaa gtatattgga
      301 cgacgtgagg ttaaataatg cgttgtctgc tatacaaacg acagtaaaac ccatcagcga
      361 gattgccagg gaaaatggtt acaagtgtcc ttctcgtttt actgaaaggt tccataatcg
      421 ttttaagata acaccaagag agctaagaaa agcgtccaga gagtaaaagt attttatgaa
      481 ggagcaacag catcgatttt tattttggaa aataaatacg gcatactcaa cctattggag
      541 cctgaacaat ccggtgactt ctgcgctaat cggggacgtt tataagca
//
LOCUS       DAJTNA010000448          574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJTNA010000448 DAJTNA010000000
VERSION     DAJTNA010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.457"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..415
                     /locus_tag="OO161_004420"
     CDS             <1..415
                     /locus_tag="OO161_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCR5465187.1"
                     /translation="NGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSK
                     ITPVSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTIT
                     VNGVATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            429..>574
                     /locus_tag="OO161_004421"
     CDS             429..>574
                     /locus_tag="OO161_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5465188.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESCGN
                     LQVSV"
BASE COUNT          152 a          131 c          174 g          117 t
ORIGIN      
        1 gaatggcacg gtggatgtgt ttcgtggctg ggtcagcagt atcggtaagg ccgtgacggc
       61 gaaagaagtg atcacccgca cggtgaaagt cactaacgtg ggtaaacctt ctgtagcgga
      121 agaacgcagc aaaattacgc cggtcagtgc gattaaggtg acgccgacat ccggtacggt
      181 ggcaaaaggg aaaacaacca ccctgacggt ttcttttgag ccggaaagtg caaccgacaa
      241 gacgttcaga gcggtttccg ccgatccgtc gaaagccacc attagtgtga aagatatgac
      301 aattacggta aacggcgtgg cgacaggtaa ggtgcagatc cctgtggtga gcggaaatgg
      361 tcagttcgcc gcagtggctg aagtcaccgt tactgaagcg ggcgctgcag ggtaaacgga
      421 ggtaatacat gtttctgaaa acagaacaat ttgaatataa cggtgtgtct gtcacgcttt
      481 ccgaattgtc tgcgctgcag cgtatcgagc atcttgccct cctgaaacgg cgtgcagaac
      541 aggcagaatc ctgcggcaac ctgcaggtaa gcgt
//
LOCUS       DAJTNA010000449          571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJTNA010000449 DAJTNA010000000
VERSION     DAJTNA010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.458"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..243)
                     /locus_tag="OO161_004422"
     CDS             complement(<1..243)
                     /locus_tag="OO161_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5465189.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKEL"
     gene            462..571
                     /locus_tag="OO161_004423"
                     /pseudo
     CDS             462..571
                     /locus_tag="OO161_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          164 a          118 c          151 g          138 t
ORIGIN      
        1 taattccttc tttctgttgg cataaaggat ggcgtcaagt tgagcgaatg aaactgaatc
       61 gggcaatggc catgcgatac cggatgcaag aaatcgctga aaaagcgtat gtattgtgga
      121 atgactgaga cccagacgct gagcgatggc ccggatggtc agtttatctt caaatcttaa
      181 acgcagggca tcaggcaaat aagaacggaa gcagggaata tcttttgttg tctgggaatt
      241 catcgttcgt gtccatcaat atagatgggc gcgattgttg ccagacagga caattttcac
      301 aagacgtcgc tgatggggcg cttaccttac cgcaggacag gtggtaactg aaaaatagtt
      361 tgctgaggcg actggaaaca gaagggcaag atcgattccg acagcagtga tggtcagcta
      421 cctcatcgat aactttgcaa cagtgccctt cttgaagcat catgcttcat gaagaaaaac
      481 tggcacgtca tcaacgtaaa caggcgatgt atacccgaat ggtagccttc ccggcggtga
      541 aaatgttcga agagtatgac ttcacgttcg t
//
LOCUS       DAJTNA010000450          560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJTNA010000450 DAJTNA010000000
VERSION     DAJTNA010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.459"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..372
                     /locus_tag="OO161_004424"
     CDS             <1..372
                     /locus_tag="OO161_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5465190.1"
                     /translation="IQPSPAQAQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVS
                     ALMTALPDHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTV
                     LRAVRDSVVSVARAVWNWIFF"
BASE COUNT          119 a          157 c          147 g          137 t
ORIGIN      
        1 attcagcctt ctcccgcaca ggcacagaat attcgtgaga aaacagatgc ggtattccgt
       61 ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg ggaactggat
      121 acgctggtca gtgcgctcat gacagcgctt cccgaccatg ccgccagtcc cctgatgacc
      181 cgcctgcagg acgagctgcg cacggaatct gtccggtctc aggcccgtga acagtttacc
      241 ggtgcggtgg accggatatt tgacacggcg gagagcgtct gtattgcctc tgttgcacgc
      301 acggttctgc gtgccgtccg tgactcggtg gtctctgttg cccgcgctgt atggaactgg
      361 attttcttct gaacagtctg ccaggggaaa tgcctgatta cattgcgtta accaactgaa
      421 aacatgacac aaacacctca tgctcctcct gtttctcttg cgtgaccgcc ttgcaatcaa
      481 tagaataaaa cgatcgataa aacaggtatc gatcgttact atcgatcgtt tatagtggtt
      541 aatggcctgc gaaaaggcag
//
LOCUS       DAJTNA010000451          550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJTNA010000451 DAJTNA010000000
VERSION     DAJTNA010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.460"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>550
                     /locus_tag="OO161_004425"
     CDS             <1..>550
                     /locus_tag="OO161_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HCR5465191.1"
                     /translation="YFQITATPHLAVYYPTVQFEFWFSEKRIADIRQVETAARYLGSA
                     LYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGEIGKTHL
                     AQELWTQIDNGQLAPDLAEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKVQVDTNN
                     NLNSMWAVKLQQMQDGRLYIAGI"
BASE COUNT          151 a          122 c          149 g          128 t
ORIGIN      
        1 tattttcaga taaccgccac gccgcatctt gccgtttatt acccgacggt acagtttgag
       61 ttctggttct cggaaaagcg gattgcggat atcaggcagg ttgaaaccgc agcccgctat
      121 cttggctcgg cgctgtactg gatagctgcc agtatcaata tcaaaccggg ccatgattat
      181 tatttttata tccgcagtgt gaatactgtt ggcaaatcgg cattcgtgga ggctgtcggt
      241 cgggcgagcg atgatgcgga aggttacctg gatttcttca aaggagaaat cgggaaaaca
      301 catctggccc aggagctgtg gacgcagatt gataacggtc agcttgcgcc ggacctggct
      361 gaaatcagga cgtccattac gaatgtcagc aatgaaatca cgcagaccgt caataaaaaa
      421 ctggaaaatc agagtgcggc aatccagcag atacagaaag ttcaggttga tacaaataat
      481 aacctgaaca gcatgtgggc cgtgaaactg cagcagatgc aggacggacg cctttatatt
      541 gcgggtatcg
//
LOCUS       DAJTNA010000452          541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJTNA010000452 DAJTNA010000000
VERSION     DAJTNA010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.461"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            145..>541
                     /locus_tag="OO161_004426"
     CDS             145..>541
                     /locus_tag="OO161_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465192.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFS"
BASE COUNT          211 a           98 c           78 g          154 t
ORIGIN      
        1 cttgacaact taataggggc acccccctgg ctattgtcat acactcaaat taaagatagg
       61 ttctaaataa atgagcgttt tttgatagtc tatttcatta ggtaatatat atttgtaaca
      121 aatcaatcaa aatggaataa aatcatgcta ccatctattt caatcaacaa taccagcgca
      181 acttacccag aatccattaa tgaaaataac aatgatgaag ttaatggatt agtacaagag
      241 ctcaaaaacc tttttaatgg taaggaagga ataagcacct gtattaaaca tctacttgag
      301 cttataaaaa acgccatacg agtaaacgac gatccttata gatttaatat taataattcc
      361 tcagttactt atattgatat tggctccaat gatacagacc atattactat tggtatcgac
      421 aaccaagaac caatagaatt acctgcgaac tataaagaca aagaactcgt ccgtactatc
      481 attaatgaca acatagttga gaagactcat gatatcaata acaaggaaat gatcttcagc
      541 g
//
LOCUS       DAJTNA010000453          527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJTNA010000453 DAJTNA010000000
VERSION     DAJTNA010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.465"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..243
                     /locus_tag="OO161_004427"
     CDS             <1..243
                     /locus_tag="OO161_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5465193.1"
                     /translation="HDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVGCFVPTLIL
                     VMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
BASE COUNT          145 a           94 c          130 g          158 t
ORIGIN      
        1 catgatcgcg ggcgttccgg cgcatgggca tttctgatga ttgtggcgtg gatgctgctg
       61 gcgggtaact gggcgatttt accgggtgtc tggcaatggg cggtggggtg ttttgtcccg
      121 acgttgattc tggtgatgat gcttatcgat cttggtgcgt ttgttggcac acaaggcgaa
      181 aataaatatg gtaaagatac tcaggatgtg aagtataaag ccgacaataa atcaagtaat
      241 taacctttaa tatatctctt actgctattt ttttccggga ttgttatttc cacgatgcaa
      301 atgacaatac ccggaaaatc cttctgaact cttcacctta agcaatatca aaaaaaatgg
      361 taatccataa gatcattact tgtttgttct tcccttaacg gcgcattatt cttaagtcgt
      421 aatcgacaga gaggcgaata tacagaggtg ccctatgaaa gatgtcgtag ataaatgcag
      481 tactaaagga tgtgcgattg atatcggtac ggtgattgat aacgaca
//
LOCUS       DAJTNA010000454          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.467, whole genome shotgun sequence.
ACCESSION   DAJTNA010000454 DAJTNA010000000
VERSION     DAJTNA010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.467"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>525
                     /locus_tag="OO161_004428"
     CDS             <1..>525
                     /locus_tag="OO161_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCR5465194.1"
                     /translation="GRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWA
                     VTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEP
                     KNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALG
                     YRSPREYLRQRACNG"
BASE COUNT          128 a          122 c          172 g          103 t
ORIGIN      
        1 ggcagagtgg ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc
       61 tgtgataacg gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca
      121 ctgcactggg cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg
      181 ggagcggtgg aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg
      241 gataatggtt catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt
      301 gaaccgaaga acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg
      361 aaaacgataa agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca
      421 aagaaccttg cagaggcgtt cgagcattat aacgaatggc atccgcatag tgcgctgggt
      481 tatcgctcgc cacgggaata tctgcggcag cgggcttgta atggg
//
LOCUS       DAJTNA010000455          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.468, whole genome shotgun sequence.
ACCESSION   DAJTNA010000455 DAJTNA010000000
VERSION     DAJTNA010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.468"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..281
                     /locus_tag="OO161_004429"
     CDS             <1..281
                     /locus_tag="OO161_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5465195.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          126 a          115 c          139 g          136 t
ORIGIN      
        1 atagcctgaa cgtgtactgt cgtaatggcc aggtggaaat cgacaacaac atcggtgaaa
       61 acgcgctgcg atcggtggcg gttggaagaa aaaattatct cttcttcggt tctgacaacg
      121 gtggagaaag cgcggcgatt atctacagtc tgctggtcac ctgcaaactg aacggagtgg
      181 agccggagga ctggttgcgc gaagtgatcg tgaagctcaa tgactggccg tcgaaccgag
      241 tgcatgaact gctgccctgg aacttctcag ctgtaaaata atccttccgc tacgtacttc
      301 tctgggcgct taccactcaa cggcatcgtc aaaattatca aagcgcagaa catagattta
      361 tcatgtgttt tatggatggc tttctccatc aggcatcgtt cgtcacggga aattggtgct
      421 atgatcggca ttgatcagtc cggttggtga tttgttttga tttggcgatt gatcagatcg
      481 cacaatccgg gctgagttcc ctctaagtga tctagg
//
LOCUS       DAJTNA010000456          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.469, whole genome shotgun sequence.
ACCESSION   DAJTNA010000456 DAJTNA010000000
VERSION     DAJTNA010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.469"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            236..516
                     /locus_tag="OO161_004430"
                     /pseudo
     CDS             236..516
                     /locus_tag="OO161_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          132 a          136 c          127 g          121 t
ORIGIN      
        1 atccccgcca gcccggctaa gatggaggca cggcacagcc cgcacacgac gctgaggcta
       61 cagagctgaa aacccacaag gccatgcccc gcatggccgg gtaaagcgga gccggcggcg
      121 acttcctttg tgctctgtcc gggaccggca ggaaaaaggc ggggataatg agatggtcac
      181 ccccacccta acagcagtac gctgttaagg tgaatccctt aacggagaga ccatcataca
      241 gaacatcaca cttattggta tcgatctcgg aaagcattcc ttccacattc attgtcaaga
      301 caagtctggt aaggctcttc tgcgtaaaaa atttacccag tactaaactg atgcagtttc
      361 tggcaacatg tccgccctca gttgttgtaa tggaagcctg tgcgggtgca cactttttgg
      421 ctcgccgtat ctctgatttt ggacatgaaa ctaagctgat ttctccacag ttcgttcgcc
      481 cgtttgtcaa aagtaataag aatgattttg ttgatg
//
LOCUS       DAJTNA010000457          506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.470, whole genome shotgun sequence.
ACCESSION   DAJTNA010000457 DAJTNA010000000
VERSION     DAJTNA010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.470"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>506
                     /locus_tag="OO161_004431"
     CDS             <1..>506
                     /locus_tag="OO161_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5465196.1"
                     /translation="RVSRAQLHVILRRTDDWKDGRRSRHSDDTDVLLRIHHVIGELPT
                     YGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKTAVPPSKRAHTGKVAVK
                     ESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFDSETVQDVMLGAVE
                     RRFGSELPT"
BASE COUNT          114 a          130 c          157 g          105 t
ORIGIN      
        1 cgggtgtcgc gtgcgcagtt gcacgtaatt ctcagacgaa ccgatgactg gaaagatggt
       61 cgccgcagcc gtcactcaga tgatacggat gtgcttctcc gtatacacca tgttatcgga
      121 gagctgccca cgtatggtta tcgtcgggta tgggcgctgc ttcgcagaca ggccgaactt
      181 gatggtatgc ctgctatcaa tgccaaacgt gtttaccgga tcatgcgcca gaatgcgctg
      241 ttgcttgagc gaaaaaccgc tgtaccgcca tcgaaacggg cacatactgg caaagtggcc
      301 gtgaaagaaa gcaatcaacg atggtgctct gacgggttcg agttccgctg tgataacgga
      361 gaaaaactgc gggtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      421 gtcactacgg gcggcttcga cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      481 cgccgcttcg gcagcgagct tccgac
//
LOCUS       DAJTNA010000458          499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.471, whole genome shotgun sequence.
ACCESSION   DAJTNA010000458 DAJTNA010000000
VERSION     DAJTNA010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.471"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>499
                     /locus_tag="OO161_004432"
     CDS             <1..>499
                     /locus_tag="OO161_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR5465197.1"
                     /translation="YQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAP
                     TLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVT
                     PGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYI
                     IHKSRE"
BASE COUNT          145 a          121 c          140 g           93 t
ORIGIN      
        1 taccagcgtt cacgctggag tacagaactg ctggcaataa aaatcaatga gataaccggt
       61 tgccagttaa atgccggaac cgttcgccgc tggttgccgt ctgcggggat tgtgtggcga
      121 agggctgcgc caactctgcg tatccgtgac ccgcataaag atgaaaagat ggcagcaatc
      181 cataaagcac tggacgaatg cagcgcagaa catccggtct tttatgaaga tgaagtggat
      241 atccatctta atcccaaaat cggtgcggac tggcaactgc gcggacagca aaaacgggtg
      301 gtcacgccgg gacagaatga aaaatattat ctggccggag cgctgcacag cgggacaggt
      361 aaagtcagct atgtgggcgg caacagcaaa agttcggcgc tgttcatcag cctgctgaag
      421 cggcttaaag cgacataccg tcgggcgaaa accatcacgc tgatcgtgga caactacatt
      481 atccacaaaa gccgggaaa
//
LOCUS       DAJTNA010000459          493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.472, whole genome shotgun sequence.
ACCESSION   DAJTNA010000459 DAJTNA010000000
VERSION     DAJTNA010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.472"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(6..81)
                     /locus_tag="OO161_004433"
     tRNA            complement(6..81)
                     /locus_tag="OO161_004433"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Lys,seq:ttt)
     gene            complement(84..159)
                     /locus_tag="OO161_004434"
     tRNA            complement(84..159)
                     /locus_tag="OO161_004434"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(124..126),aa:Val,seq:tac)
     gene            complement(195..270)
                     /locus_tag="OO161_004435"
     tRNA            complement(195..270)
                     /locus_tag="OO161_004435"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Lys,seq:ttt)
     gene            complement(274..349)
                     /locus_tag="OO161_004436"
     tRNA            complement(274..349)
                     /locus_tag="OO161_004436"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Val,seq:tac)
BASE COUNT          116 a          138 c          127 g          112 t
ORIGIN      
        1 aaaactggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
       61 taccgactga gctaacgacc cggtggtggg tgatgacggg atcgaaccgc cgaccccctc
      121 cttgtaaggg aggtgctctc ccagctgagc taatcaccca atactacgct ggatactaca
      181 actaactatg ttagtggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
      241 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
      301 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
      361 ggatactata aactacttcc accgaaccca tcgtaattag tggtgggtcg ggcaggatga
      421 ctcgcttcgc tcgccctacg ggccgttgct gacgcaacgt tatcctacac gcttgacatc
      481 tgtcttcgat gct
//
LOCUS       DAJTNA010000460          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.473, whole genome shotgun sequence.
ACCESSION   DAJTNA010000460 DAJTNA010000000
VERSION     DAJTNA010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.473"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>488
                     /locus_tag="OO161_004437"
     CDS             <1..>488
                     /locus_tag="OO161_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISSbo1 family transposase"
                     /protein_id="HCR5465198.1"
                     /translation="TNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTL
                     WPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTC
                     GGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSHITTESQWRSLV
                     LKA"
BASE COUNT          105 a          127 c          154 g          102 t
ORIGIN      
        1 actaactcat gcggcagccg tgcctgcccg tcctgcggaa aaaaggccac agacctgtgg
       61 atagcgacac agctgaatcg tcttcctgac tgcgactggg tacacctggt cttcaccctg
      121 ccggacacgc tgtggccggt gttcgaaagc aaccggtggc tgctgaatga cgtgtgccgt
      181 ctggcggtgg agaatctgct gtatgccgcc cggaaacggg ggcaggaacc cggtatcttc
      241 tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc cgcatgtaca tgtgtctgta
      301 acctgtggag gtctgaataa gcatggtcag tggaaaaagc tgagcttcct gaaagacgcg
      361 atgcgttcac ggtggatgtg gaatatgcgg cagctgcttc tgaaagcgtg gtcagagggg
      421 ctggcgatgc cggagtcgtt gtcacatatc acgacggaat cacagtggag aagcctggtg
      481 ctgaaagc
//
LOCUS       DAJTNA010000461          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.475, whole genome shotgun sequence.
ACCESSION   DAJTNA010000461 DAJTNA010000000
VERSION     DAJTNA010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.475"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>463
                     /gene="ipaH9.8"
                     /locus_tag="OO161_004438"
     CDS             <1..>463
                     /gene="ipaH9.8"
                     /locus_tag="OO161_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5465199.1"
                     /translation="LDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADA
                     TESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVR
                     TLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVR"
BASE COUNT          104 a          132 c          120 g          107 t
ORIGIN      
        1 cttgaccgcc tttccgatac cgtctctgca cgcaatacct ccggattccg tgaacaggtc
       61 gctgcatggc tggaaaaact cagtgcctct gcggagcttc gacagcagtc tttcgctgtt
      121 gctgctgatg ccactgagag ctgtgaggac cgtgtcgcgc tcacatggaa caatctccgg
      181 aaaaccctcc tggtccatca ggcatcagaa ggccttttcg ataatgatac cggcgctctg
      241 ctctccctgg gcagggaaat gttccgcctc gaaattctgg aggacattgc ccgggataaa
      301 gtcagaactc tccattttgt ggatgagata gaagtctacc tggccttcca gaccatgctc
      361 gcagagaaac ttcagctctc cactgccgtg aaggaaatgc gtttctatgg cgtgtcggga
      421 gtgacagcaa atgacctccg cactgccgaa gccatggtca gaa
//
LOCUS       DAJTNA010000462          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.476, whole genome shotgun sequence.
ACCESSION   DAJTNA010000462 DAJTNA010000000
VERSION     DAJTNA010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.476"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..316)
                     /locus_tag="OO161_004439"
     CDS             complement(<1..316)
                     /locus_tag="OO161_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5465200.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDADWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDV"
     gene            complement(324..>461)
                     /locus_tag="OO161_004440"
     CDS             complement(324..>461)
                     /locus_tag="OO161_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5465201.1"
                     /translation="PAMTAIQALAGLITTMVTQGYEYRRDDDMALWSSADLTYSITYE
                     M"
BASE COUNT           93 a          147 c          103 g          118 t
ORIGIN      
        1 ggacatcgcc gctttcaaac cagcctataa gccctttctg accttcctct cccggtttcc
       61 aggccagcgt aaaactggta tcacctgcag atttctgccc ctgcccggtc gcggtccagt
      121 ctgcgtcttc atcatccagg tagttatcat cgtaggattc tgccgtcatc tcgcccggcg
      181 tcagatcctt caccttagcc agtcgctgcc agtcagcgtc tgacaacggg tttgcataag
      241 catcaccctt gccgttgtaa acccacagag tggtaccggc accttttact ggctccaggg
      301 gatttggtgt tgccatatcg tcctcacatc tcgtatgtaa tggaataagt cagatccgca
      361 gagctccata acgccatatc gtcatcacga cgatactcat agccctgcgt aaccatcgtg
      421 gtaatcagtc ctgccagtgc ctggatcgca gtcatcgccg g
//
LOCUS       DAJTNA010000463          458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.477, whole genome shotgun sequence.
ACCESSION   DAJTNA010000463 DAJTNA010000000
VERSION     DAJTNA010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.477"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..458
                     /locus_tag="OO161_004441"
                     /pseudo
     CDS             1..458
                     /locus_tag="OO161_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          110 a          114 c          133 g          101 t
ORIGIN      
        1 caataccagg aaggaagtct tactgctgtg gccgccggag aacaggttgt tcctgcctct
       61 gaacttgctg ccgccatgaa gcagattaaa gaactccagc gcctgctcgg caagaaaacg
      121 atggaaaatg aactcctcaa agaagccgtt gaatatggac gggcaaaaaa gtggatagcg
      181 cacgcgccct tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt
      241 cgcgtgcgca gttgcacgtc attctcagac gaaccgatga ctggatggat ggccgccgca
      301 gtcgtcacac tgatgatacg gatgtgcttc tccgtataca ccatgttatc ggagagctgc
      361 ccacgtatgg ttatcgtcgg gtatgggcgc tgcttcgcag acaggcagaa cttgatggta
      421 tgcctgcgat caatgccaaa cgtgtttacc ggatcatg
//
LOCUS       DAJTNA010000464          453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.478, whole genome shotgun sequence.
ACCESSION   DAJTNA010000464 DAJTNA010000000
VERSION     DAJTNA010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.478"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..348
                     /locus_tag="OO161_004442"
                     /pseudo
     CDS             1..348
                     /locus_tag="OO161_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            345..>453
                     /locus_tag="OO161_004443"
     CDS             345..>453
                     /locus_tag="OO161_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5465202.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQ"
BASE COUNT          118 a          130 c          122 g           83 t
ORIGIN      
        1 cagggcacag actaaaggta aggtggaacg gatggtgcag tacacccgta acagttttta
       61 catcccacta atgactcgcc tgcgcccgat ggggagcact gtcgatgttg aaacagccaa
      121 ccgccacggt ctgcgctggc tgcacgatgt cgctaaccaa cgaaagcatg aaacaatcca
      181 ggcccgtccc tgtgatcgct ggctcgaaga gcagcagtcc atgctggcac tgcctccgga
      241 gaaaaaagag tatgacgtgc atcctggtga aaatctggtg agcttcgaca acccccccag
      301 catcatccac tctccatcta cgactcattc tgcagaggag tggcgtgatg atggaactgc
      361 aacatcaacg actgatggcg ctcgccgggc agttgcaact ggaaagcctt ataagcgcag
      421 cgcctgcgct gtcacaacag gcagtagacc agg
//
LOCUS       DAJTNA010000465          452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.479, whole genome shotgun sequence.
ACCESSION   DAJTNA010000465 DAJTNA010000000
VERSION     DAJTNA010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.479"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>452
                     /locus_tag="OO161_004444"
     CDS             <1..>452
                     /locus_tag="OO161_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5465203.1"
                     /translation="NDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEY
                     PQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAA
                     GKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEA"
BASE COUNT          119 a          113 c          121 g           99 t
ORIGIN      
        1 aatgacgaat tatatcttga tggcaacgct cgtcagaacc tagccacttt ctgccagacc
       61 tgggacgacg aaaatgtcca caaattgatg gatttatcca ttaacaaaaa ctggatcgac
      121 aaagaagaat atccgcaatc cgcagccatc gacctgcgtt gcgtaaatat ggttgccgat
      181 ctgtggcatg cgcctgcgcc gaaaaatggt caggccgttg gcaccaacac cattggttct
      241 tccgaggcct gtatgctcgg cgggatggcg atgaaatggc gttggcgcaa gcgtatggaa
      301 gctgcaggca aaccaacgga taaaccaaac ctggtgtgcg gtccggtaca aatctgctgg
      361 cataaattcg cccgctactg ggatgtggag ctgcgtgaga tccctatgcg ccccggtcag
      421 ttgtttatgg acccgaaacg catgattgaa gc
//
LOCUS       DAJTNA010000466          443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.480, whole genome shotgun sequence.
ACCESSION   DAJTNA010000466 DAJTNA010000000
VERSION     DAJTNA010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.480"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>443
                     /locus_tag="OO161_004445"
     CDS             <1..>443
                     /locus_tag="OO161_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5465204.1"
                     /translation="ARYIKKSNSVFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSG
                     YYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARL
                     RKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVA"
BASE COUNT          136 a          105 c          115 g           87 t
ORIGIN      
        1 gcgagatata ttaaaaaaag caacagcgta ttttgcacag gagtcgctga aaaatacgcg
       61 ttaatcgaac aatggcgaca acaatttccc attgaagcga tgtgtcaggt atttggtgta
      121 tccaggagcg gttattacaa ctgggtacag catgaaccct cagacagaaa acaaagtgat
      181 gagcggctaa aactggagat taaggtggca catatccgca ctcgcgaaac atatggaacc
      241 cggcggctcc agacggagct ggcagagaat ggcatcatcg ttggtcgtga ccgactggca
      301 cgtcttcgta aggagctaag gctacgctgt aagcagaaac gcaagttcag agcgactacg
      361 aacccgaacc acaatctgcc agttgcgcca aatctgctga accagacgtt cgctcctaca
      421 gcaccaaatc aggtctgggt ggc
//
LOCUS       DAJTNA010000467          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.481, whole genome shotgun sequence.
ACCESSION   DAJTNA010000467 DAJTNA010000000
VERSION     DAJTNA010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.481"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..>442)
                     /locus_tag="OO161_004446"
     CDS             complement(<1..>442)
                     /locus_tag="OO161_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5465205.1"
                     /translation="YITQDPIGLKGGWIFYQYPLNPVINVDPQGLVDINLYPESDLIH
                     SVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCN
                     TSKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLT"
BASE COUNT          127 a          103 c           73 g          139 t
ORIGIN      
        1 ctgttagcac tgtatccatt tttaacttac cattagttcc tcgcccccac cacgtccata
       61 gcgtgtcagg tcctattaca tttgtatgta actctttagc taattggcta gcaaatgaat
      121 tttgtccttt acttgtatta caagaaaata accaaactgt catcccatct ttataattcc
      181 catcaaattt aattagatat gctagatctt tagctgtcat gatactgcgc gttgcggatt
      241 caatagatgt gggggtacca tgccccccga ttgtgaaaac gcctgggata ttaatctcat
      301 cagctacaga atggataaga tcactttcgg ggtataaatt tatatcaacc aaaccttgcg
      361 gatctacatt tatgaccgga ttcaacggat actgataaaa aatccatccc cccttcagcc
      421 caatcggatc ctgagtgata ta
//
LOCUS       DAJTNA010000468          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.482, whole genome shotgun sequence.
ACCESSION   DAJTNA010000468 DAJTNA010000000
VERSION     DAJTNA010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.482"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..173)
                     /locus_tag="OO161_004447"
     CDS             complement(<1..173)
                     /locus_tag="OO161_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCR5465206.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQA"
     gene            complement(178..>438)
                     /locus_tag="OO161_004448"
     CDS             complement(178..>438)
                     /locus_tag="OO161_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5465207.1"
                     /translation="SASFVLSTPTETDGAVFPGRIMLANTCTWTYRGDECGYHGPAVA
                     DEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLFINKLSQ"
BASE COUNT           98 a          133 c          112 g           95 t
ORIGIN      
        1 tctgcctgca gccagtcctc cggagccatc cggaaataat cctccggctc accggaaata
       61 ttcacgcagg gaaaatatct ttccccctcc ggcgctctca ccacgaagcc gcacgactcc
      121 gctggcgcac atcgccgggc gtgcgccaga atcgctgatt ctgtctctgt catgggatta
      181 ctgcgaaagt ttgttaatga aaaggaagcc gccaaagttg ccgacgttat tgcgaaactt
      241 acagccgctc aggcatttgc tgcatttatc cttcgtgata tcggacgtcg gctggtcata
      301 ttcatccgcg actgccggac cgtgataacc gcactcatca ccgcgatagg tccaggtgca
      361 ggtgttggcc agcatgatac gtcccggaaa aacagcgcca tccgtttccg tcggcgtgga
      421 cagtacaaag gaggcact
//
LOCUS       DAJTNA010000469          423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.483, whole genome shotgun sequence.
ACCESSION   DAJTNA010000469 DAJTNA010000000
VERSION     DAJTNA010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.483"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            38..319
                     /locus_tag="OO161_004449"
                     /pseudo
     CDS             38..319
                     /locus_tag="OO161_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT           89 a          120 c          102 g          112 t
ORIGIN      
        1 aaaataatgt ccatcatttt gaagtggaca ctatcgtatg gaataccgga cctggatcac
       61 tgaagcttta cgccttcatt tcgaagaaca tttaccacgg gttgtggccg gacgtcgcct
      121 gggcgtacca aaatcaactg tctgtggtat gttcgtgcgc ttccgcaacg ctggcctttc
      181 atggcctctg cccgcaggta tgtcggagca ggaacttgat gcccttcttt acggaagtgc
      241 ctccacagta cctgtcgtac ttactgaaag caccgttatg cccaaactcc cggtggtaaa
      301 aaaacgtccc cggcggcctt aatgccagtc agtaaagcaa ctgactggct ctttttcggg
      361 gctgtggggt atttccaggg cctctccttt accactctcg ggaaggccct tccccttctc
      421 gtc
//
LOCUS       DAJTNA010000470          417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.484, whole genome shotgun sequence.
ACCESSION   DAJTNA010000470 DAJTNA010000000
VERSION     DAJTNA010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.484"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            90..359
                     /locus_tag="OO161_004450"
     CDS             90..359
                     /locus_tag="OO161_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR5465208.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQQLRQNLEARLDAEVDS
                     ILDELLGAPTVPEPEGIADDSAVSDGVGSQPDGSEGPQPDGEMMM"
BASE COUNT          104 a          103 c          123 g           87 t
ORIGIN      
        1 cagcgccagt cagtcagccg gagcaaaaac agaagcccca ctcattccgg aacaaccttc
       61 ttaataaccc atttcaacga gaaaatccta tgtcagaaat aaaatcgctg gtcactgctg
      121 aggcagtgaa ggaagtcctg cgctctgaag aagtccggag cgcactgaaa cagcaacttc
      181 ggcagaacct tgaggcgcgt cttgatgcag aagtggattc aattctggat gaactgctgg
      241 gcgcaccgac tgttccggag ccggaaggca ttgcggatga cagtgctgtt tcagatggcg
      301 tcgggtctca gcctgacggt agtgaagggc ctcagcctga cggcgaaatg atgatgtaac
      361 catgcgcagg gggtgtcggt gtgagctgat gccccacttg ttgttgtgag cttccgg
//
LOCUS       DAJTNA010000471          411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.487, whole genome shotgun sequence.
ACCESSION   DAJTNA010000471 DAJTNA010000000
VERSION     DAJTNA010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.487"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>411
                     /locus_tag="OO161_004451"
     CDS             <1..>411
                     /locus_tag="OO161_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5465209.1"
                     /translation="GWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDVVKRFEE
                     MVVQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAENARQDATVTAEDRKATAEDV
                     TSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAAL"
BASE COUNT          110 a          107 c          135 g           59 t
ORIGIN      
        1 gggctggcac aacgagtaca acgttggcga cattgctgta tatgaggact caaagcccgg
       61 cacgctgaat gactttctga ttgctcctga tgagggcgac ctgaaaccgg atgtcgtcaa
      121 acgctttgag gaaatggtgg tgcaggcgca gcagagcgcc ggggccgcag ccggaaacgc
      181 acagcagacg gcgcaggatg tggcggcagc cgcaacggcc cgtgatgatg cacaacgttt
      241 tgcggagaat gccagacagg atgcaaccgt cacagctgag gacagaaagg ccactgcgga
      301 agatgtgaca agcacaggag caaatgcagc cgcagccgga cagagcgcac aggatgccgc
      361 aggttatgcc cgcgcagcag aacaggccaa aaatgacatt gatgctgcgc t
//
LOCUS       DAJTNA010000472          409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.488, whole genome shotgun sequence.
ACCESSION   DAJTNA010000472 DAJTNA010000000
VERSION     DAJTNA010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.488"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>409
                     /locus_tag="OO161_004452"
     rRNA            <1..>409
                     /locus_tag="OO161_004452"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          100 a          104 c          122 g           83 t
ORIGIN      
        1 atgtgccttc gggaaccgtg agacaggtgc tgcatggctg tcgtcagctc gtgttgtgaa
       61 atgttgggtt aagtcccgca acgagcgcaa cccttatcct ttgttgccag cggtccggcc
      121 gggaactcaa aggagactgc cagtgataaa ctggaggaag gtggggatga cgtcaagtca
      181 tcatggccct tacgaccagg gctacacacg tgctacaatg gcgcatacaa agagaagcga
      241 cctcgcgaga gcaagcggac ctcataaagt gcgtcgtagt ccggattgga gtctgcaact
      301 cgactccatg aagtcggaat cgctagtaat cgtggatcag aatgccacgg tgaatacgtt
      361 cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaa
//
LOCUS       DAJTNA010000473          404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.489, whole genome shotgun sequence.
ACCESSION   DAJTNA010000473 DAJTNA010000000
VERSION     DAJTNA010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.489"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            63..386
                     /locus_tag="OO161_004453"
     CDS             63..386
                     /locus_tag="OO161_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5465210.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
BASE COUNT           84 a           94 c          147 g           79 t
ORIGIN      
        1 gatgcctccg tgtaaggggg atttctgttc aggaggtaat gataccgatg aaacgcgaga
       61 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgctgtgagg
      121 gtaaacagct ggcggtatgg atgcggcggg tctgcctggg tgagccggtt gcccgttccg
      181 ggaaactgcc gacactggca ccgccgttac tgcgtcagct ggctgccatc gggaataacc
      241 tgaatcagac agcgaggaag gtgaacagcg ggcagtggtc ttccggtaac cgggttcagg
      301 tggtggccgc actgatggcc atcggggacg agctgcgccg gctgcgtctg gccgtcaggg
      361 aacaggggac gcgggatgat agttaagttt catgcccggg gaaa
//
LOCUS       DAJTNA010000474          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.490, whole genome shotgun sequence.
ACCESSION   DAJTNA010000474 DAJTNA010000000
VERSION     DAJTNA010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.490"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            186..>391
                     /locus_tag="OO161_004454"
     CDS             186..>391
                     /locus_tag="OO161_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465211.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWR"
BASE COUNT          100 a           77 c          121 g           93 t
ORIGIN      
        1 ttttatgcca ttaccggttt taaccaacag gattatcggt gggcatgaaa gaaaaccccg
       61 gtatctgctg ataccggggt ttctctttag catggcagaa atgtgtttca tgcttttcgg
      121 gcgaaggata tccgacttct gtacggaatg gcaagtggcg gttaatttat tcaggggaag
      181 gctgtatggg aaaaggtggc ggtaaggcac acactcctcg tgaggcgaag gataatctca
      241 aatccacgca gatgatgagt gtgattgatg cgattggtga gggaccgata gaaggtccgg
      301 tgaagggact gcagagtatt ctggtgaaca aaaccccact gacggacacg gacggcaatc
      361 ccgtgataca cggtgtgacg gcggtctggc g
//
LOCUS       DAJTNA010000475          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.491, whole genome shotgun sequence.
ACCESSION   DAJTNA010000475 DAJTNA010000000
VERSION     DAJTNA010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.491"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>387
                     /locus_tag="OO161_004455"
     CDS             <1..>387
                     /locus_tag="OO161_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5465212.1"
                     /translation="WAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPG
                     GVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVG
                     YVSFSEAAHAITDYIVGYYSALRPHE"
BASE COUNT           95 a           96 c          120 g           76 t
ORIGIN      
        1 gctgggcgta cctcgccgtt gttctcgacc tgttcgcaag aaaaccagtg ggctgggcca
       61 tgtcgttctc gccggacagc aggctcacca tgaaagcgct ggaaatggca tgggaaaccc
      121 gtggtaagcc cggcggggtg atgttccaca gcgatcaggg cagtcattat acgagcaggc
      181 agttccggca gttattgtgg cgataccaga tcagacagag tatgagccgg cgcggaaact
      241 gctgggataa cagcccaatg gaacgcttct tcaggagtct gaagaacgaa tggatgccgg
      301 tggtgggtta cgtaagcttc agcgaggcag ctcacgccat aacggactat atcgttggat
      361 attacagcgc actaagaccg cacgaat
//
LOCUS       DAJTNA010000476          384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.492, whole genome shotgun sequence.
ACCESSION   DAJTNA010000476 DAJTNA010000000
VERSION     DAJTNA010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.492"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..77)
                     /gene="hokE"
                     /locus_tag="OO161_004456"
     CDS             complement(<1..77)
                     /gene="hokE"
                     /locus_tag="OO161_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5465213.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVG"
BASE COUNT          105 a          106 c           78 g           95 t
ORIGIN      
        1 tctccgacca gaagcgtaaa tcccagtacc gtcaaacaca gcactatgac tgccacaagg
       61 gcatatttcg tcagcatgtt tatattgcct ccatgcgaag aagggactac catccgagtt
      121 gttgaggctc ggagtggcag ccccaggatt gatagaaata tctcctgggg cttcatcttt
      181 ccagacctca gagttaacct gaaaccggaa agctttaggc acccgccgtt atcttaccct
      241 tcctcaacaa aatagctgtt tatttataca gtaaatcctg tttcggactt ctgtcggcaa
      301 aactcatcat tgctcttaaa acgaaatcgc ccgcagccat cactgaccgc gggcgaaaat
      361 gtaaagcagg tgccttacca tcct
//
LOCUS       DAJTNA010000477          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.493, whole genome shotgun sequence.
ACCESSION   DAJTNA010000477 DAJTNA010000000
VERSION     DAJTNA010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.493"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>364
                     /locus_tag="OO161_004457"
     CDS             <1..>364
                     /locus_tag="OO161_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5465214.1"
                     /translation="GKLLNGKGTVAIISYPALQSVVARVDGFKKGLSDTPDIKIVAEQ
                     PSITRAEALTTAQNIMQANANLNGLFGFGDDAALAAVIAAKSAHNDNIKIIGFDGMKE
                     ARDAVDSEKTFAAVIRQYP"
BASE COUNT          100 a           72 c           98 g           94 t
ORIGIN      
        1 gggcaaactt ttgaatggta aaggtactgt agccattatt tcttatccgg cactacaatc
       61 ggtggttgcg cgcgttgatg gttttaagaa agggttaagc gatacaccgg atattaaaat
      121 tgttgctgaa cagcctagta ttacgcgggc tgaagcgtta acgactgcgc aaaacattat
      181 gcaggcaaat gccaacctga atggtctgtt tggctttggt gatgatgcgg cgctggcagc
      241 ggttatcgca gcaaaatctg cgcataacga caacatcaaa atcattggtt ttgacggcat
      301 gaaagaagcg cgtgatgccg ttgacagtga aaaaacgttt gcggccgtga ttcggcaata
      361 tccc
//
LOCUS       DAJTNA010000478          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.494, whole genome shotgun sequence.
ACCESSION   DAJTNA010000478 DAJTNA010000000
VERSION     DAJTNA010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.494"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
BASE COUNT           96 a          107 c           84 g           73 t
ORIGIN      
        1 tgaaccgggc tataacctgc agtcggttgt tgaacgcctg acaacaggaa atcagccagg
       61 ccagcaacca cgcggactga cagagaatca gaacctgaat cagcctgaaa tcatacccgg
      121 ggcagtactt catcaccagt caccaatacc cgtgtcagta cttcgcggcc tgacgcgatc
      181 acccgactca gtacttctga gcccgcctcc ggtaccattc tctgtgggcc cgtacacaac
      241 aacagaaaag ggtgcccggc ccggggcgca accgtattac acttccggaa aatcgttata
      301 cgcagaaaaa tcggatcacg ctgtgcctgc tttcatgtaa tttacttacg gtgttataag
//
LOCUS       DAJTNA010000479          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.495, whole genome shotgun sequence.
ACCESSION   DAJTNA010000479 DAJTNA010000000
VERSION     DAJTNA010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.495"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>353
                     /locus_tag="OO161_004458"
     CDS             <1..>353
                     /locus_tag="OO161_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465215.1"
                     /translation="MNDVFLKRLTAPTITSGGDPPAFSLTPDGRLTAKNADISGNVNA
                     NSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYD
                     DQPFDRQIVIPAVAF"
BASE COUNT           83 a           99 c          100 g           71 t
ORIGIN      
        1 atgaacgacg tgttcctgaa acgcctgacg gctcccacca ttaccagcgg cggtgatcct
       61 ccggcatttt ccctgacacc ggacgggcgg ctgacggcga aaaatgccga tattagcggt
      121 aacgtgaatg cgaactccgg gacgctcaac aacgtcacga ttaacgagaa ctgtcgggtt
      181 ctgggaaaac tgtccgccaa ccagattgaa ggcgatctcg ttaaaacagt gggcaaagct
      241 ttcccccggg actcccgtgc accggagcgg tggccatcag ggaccattac cgtcagggtt
      301 tatgacgatc agccgtttga ccggcaaatt gttattccgg cggtggcatt cag
//
LOCUS       DAJTNA010000480          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.496, whole genome shotgun sequence.
ACCESSION   DAJTNA010000480 DAJTNA010000000
VERSION     DAJTNA010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.496"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            1..319
                     /locus_tag="OO161_004459"
                     /pseudo
     CDS             1..319
                     /locus_tag="OO161_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           97 a           68 c          121 g           67 t
ORIGIN      
        1 tttcagcagg gaaaccgatg gcttaactgg ctggagaagc agccggtgca gatgagccgc
       61 ctggaacatt atgccggtca ggatgaaatt ggcctgcgtt acaacagcca ccggacaaaa
      121 cgagaagaaa accttgtgat gagtggcgac gagtttatgg aaaggttctc ctggcatgtg
      181 gcggataagg ggttccggat ggtgatacgg ggtcctgagt ccggtgaagc ggcgattact
      241 ggaagatgtg gtgtacgcca taacggagac agtgagaaaa acggcgaggc aaatcacaag
      301 gagagggatg tatcagcagt gactgaaggt tgacccgctg aagtgcgagc tgt
//
LOCUS       DAJTNA010000481          352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.497, whole genome shotgun sequence.
ACCESSION   DAJTNA010000481 DAJTNA010000000
VERSION     DAJTNA010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.497"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>352
                     /locus_tag="OO161_004460"
     CDS             <1..>352
                     /locus_tag="OO161_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5465216.1"
                     /translation="GGQATGKTWLAFTNVGNSNLGVATSGQGIRVVDAQNGATTEEGA
                     FALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTSMLTQAMDYDRILAGSRS
                     HQTGVNGENNSVRL"
BASE COUNT           87 a           97 c           99 g           69 t
ORIGIN      
        1 atggtggtca ggcaaccggc aaaacctggc ttgcgtttac aaatgtcgga aacagtaacc
       61 tcggggtggc aacctccgga cagggtatcc gggttgtgga tgcacagaat ggcgccacca
      121 cagaagaagg tgcgtttgcc ctgagtcgcc cgcttcaggc cggcgccttt aactacaccc
      181 tgaaccgtga cagcgatgaa gactggtacc tgcgcagtga aaatgcttat cgtgctgaag
      241 tccccctgta tacatccatg ctgacacagg caatggacta tgaccggatt ctggcaggct
      301 cccgcagcca tcagaccggt gtaaacggtg aaaataacag cgtccgtctc ag
//
LOCUS       DAJTNA010000482          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.503, whole genome shotgun sequence.
ACCESSION   DAJTNA010000482 DAJTNA010000000
VERSION     DAJTNA010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.503"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..270
                     /locus_tag="OO161_004461"
     CDS             <1..270
                     /locus_tag="OO161_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCR5465217.1"
                     /translation="WNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALKVGES
                     ILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
BASE COUNT          105 a           46 c           78 g           88 t
ORIGIN      
        1 tggaatacat ggaatcagac ggggcttgaa ttaggctggt ttaagcaaca gaacaaaact
       61 cagcagggtg taacactaaa tgaatcggct tataaaacga cactctggca tgcattgaaa
      121 gtgggtgaaa gcattttagg ttcacgacca gaaattcgct tctatggaac gtatatcaat
      181 attctggata acgaattatc gaattttaag tttaatgaga acagcaaaga cgagtttatg
      241 gccggcatcc aggcggaagt ctggtggtaa tctattttag ttaaggagtt gtgtatgtca
      301 ggatttaaaa aaggttt
//
LOCUS       DAJTNA010000483          315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.504, whole genome shotgun sequence.
ACCESSION   DAJTNA010000483 DAJTNA010000000
VERSION     DAJTNA010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.504"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..>315
                     /locus_tag="OO161_004462"
     CDS             <1..>315
                     /locus_tag="OO161_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5465218.1"
                     /translation="NTSEVVMRTRADVVTGNNGEYSFEAQVGKYCVYLKRDWRDEYCV
                     GDIAVYDDSKPGTLNDFLTAPDEGDLKPDVVKRFEEMVAQAQQSAGAAAGNAQQTAQD
                     VAA"
BASE COUNT           79 a           75 c          105 g           56 t
ORIGIN      
        1 aaacacctca gaagtggtga tgcgtaccag ggctgatgta gtgaccggaa ataacggtga
       61 atattcgttt gaggcacagg tcggaaaata ttgcgtgtat ctgaaacggg actggcgcga
      121 cgagtactgt gttggcgaca ttgctgtata cgacgactca aagcccggca cgctgaatga
      181 ctttctgact gcccccgatg aaggcgacct gaagccggat gtcgtcaaac gctttgagga
      241 aatggtggcg caggcgcagc agagcgccgg ggccgcagcc ggaaacgcac agcagacggc
      301 gcaggatgtg gcggc
//
LOCUS       DAJTNA010000484          307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.denovo.507, whole genome shotgun sequence.
ACCESSION   DAJTNA010000484 DAJTNA010000000
VERSION     DAJTNA010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.denovo.507"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     misc_feature    39..155
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            84..>307
                     /locus_tag="OO161_004463"
     CDS             84..>307
                     /locus_tag="OO161_004463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5465219.1"
                     /translation="MPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHHDKRSARA
                     QLDDWLKKEILRVYDENHQVYAVRKVRHQL"
BASE COUNT           81 a           76 c           81 g           69 t
ORIGIN      
        1 gaactgcgcc gcagtaacaa tatccttcgc ctggcttccg cttattttgc aaaggcggag
       61 ttcgaccgcc tctggaaaaa ataatgccac tgctggataa gctgcgtgag cagtacgggg
      121 tcggaccggt atgcagtgaa ctgcatattg ccccgtcaac gtattaccac tgtcagcaac
      181 agcgacatca tcatgataaa cgcagtgccc gtgcgcagct cgatgactgg ctgaagaaag
      241 agatactgcg cgtatacgat gagaatcatc aggtatacgc tgtgcgtaaa gtcaggcatc
      301 agttgtt
//
LOCUS       DAJTNA010000485        26596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.guided.040, whole genome shotgun sequence.
ACCESSION   DAJTNA010000485 DAJTNA010000000
VERSION     DAJTNA010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26596
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.guided.040"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            561..800
                     /locus_tag="OO161_004464"
     CDS             561..800
                     /locus_tag="OO161_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5465220.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(988..1965)
                     /gene="intI2"
                     /locus_tag="OO161_004465"
                     /pseudo
     CDS             complement(988..1965)
                     /gene="intI2"
                     /locus_tag="OO161_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2297..2770
                     /locus_tag="OO161_004466"
     CDS             2297..2770
                     /locus_tag="OO161_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5465221.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2865..3390
                     /gene="sat2"
                     /locus_tag="OO161_004467"
                     /pseudo
     CDS             2865..3390
                     /gene="sat2"
                     /locus_tag="OO161_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
     gene            3459..3956
                     /locus_tag="OO161_004468"
     CDS             3459..3956
                     /locus_tag="OO161_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465222.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4017..4388
                     /locus_tag="OO161_004469"
     CDS             4017..4388
                     /locus_tag="OO161_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465223.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4798..5175
                     /locus_tag="OO161_004470"
     CDS             4798..5175
                     /locus_tag="OO161_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465224.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5198..5350
                     /locus_tag="OO161_004471"
                     /pseudo
     CDS             5198..5350
                     /locus_tag="OO161_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5406..7022)
                     /locus_tag="OO161_004472"
     CDS             complement(5406..7022)
                     /locus_tag="OO161_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5465225.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(7023..8549)
                     /locus_tag="OO161_004473"
     CDS             complement(7023..8549)
                     /locus_tag="OO161_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5465226.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8552..10219)
                     /locus_tag="OO161_004474"
     CDS             complement(8552..10219)
                     /locus_tag="OO161_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5465227.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10216..12324)
                     /locus_tag="OO161_004475"
     CDS             complement(10216..12324)
                     /locus_tag="OO161_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5465228.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPLLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12311..13132)
                     /locus_tag="OO161_004476"
     CDS             complement(12311..13132)
                     /locus_tag="OO161_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5465229.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13292..15121)
                     /gene="glmS"
                     /locus_tag="OO161_004477"
     CDS             complement(13292..15121)
                     /gene="glmS"
                     /locus_tag="OO161_004477"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5465230.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRIGHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTELLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15283..16653)
                     /gene="glmU"
                     /locus_tag="OO161_004478"
     CDS             complement(15283..16653)
                     /gene="glmU"
                     /locus_tag="OO161_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5465231.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILMF
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(17003..17422)
                     /gene="atpC"
                     /locus_tag="OO161_004479"
     CDS             complement(17003..17422)
                     /gene="atpC"
                     /locus_tag="OO161_004479"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5465232.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17443..18825)
                     /gene="atpD"
                     /locus_tag="OO161_004480"
     CDS             complement(17443..18825)
                     /gene="atpD"
                     /locus_tag="OO161_004480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5465233.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18852..19715)
                     /gene="atpG"
                     /locus_tag="OO161_004481"
     CDS             complement(18852..19715)
                     /gene="atpG"
                     /locus_tag="OO161_004481"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5465234.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19766..21307)
                     /gene="atpA"
                     /locus_tag="OO161_004482"
     CDS             complement(19766..21307)
                     /gene="atpA"
                     /locus_tag="OO161_004482"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5465235.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21320..21853)
                     /gene="atpH"
                     /locus_tag="OO161_004483"
     CDS             complement(21320..21853)
                     /gene="atpH"
                     /locus_tag="OO161_004483"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5465236.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21868..22338)
                     /gene="atpF"
                     /locus_tag="OO161_004484"
     CDS             complement(21868..22338)
                     /gene="atpF"
                     /locus_tag="OO161_004484"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5465237.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAYKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22400..22639)
                     /gene="atpE"
                     /locus_tag="OO161_004485"
     CDS             complement(22400..22639)
                     /gene="atpE"
                     /locus_tag="OO161_004485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5465238.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22686..23501)
                     /gene="atpB"
                     /locus_tag="OO161_004486"
     CDS             complement(22686..23501)
                     /gene="atpB"
                     /locus_tag="OO161_004486"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5465239.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23510..23890)
                     /gene="atpI"
                     /locus_tag="OO161_004487"
     CDS             complement(23510..23890)
                     /gene="atpI"
                     /locus_tag="OO161_004487"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5465240.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(24507..25130)
                     /gene="rsmG"
                     /locus_tag="OO161_004488"
     CDS             complement(24507..25130)
                     /gene="rsmG"
                     /locus_tag="OO161_004488"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5465241.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQRPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(25194..>26596)
                     /gene="mnmG"
                     /locus_tag="OO161_004489"
     CDS             complement(25194..>26596)
                     /gene="mnmG"
                     /locus_tag="OO161_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5465242.1"
                     /translation="HIGLDNYSGGRAGDPPSIPLSHRLRELPLRVGRLKTGTPPRIDA
                     RTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMY
                     AGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQ
                     IVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAA
                     AQGLLAGLNAARLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLML
                     REDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNA
                     HLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQ
                     QDEIEKQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAIS
                     ILLVWLKKQGMLRRSA"
BASE COUNT         6892 a         6714 c         6025 g         6965 t
ORIGIN      
        1 aaagagccag tcagttgctt aactgactgg cattacaccg gtcggcagcc ctgtgggcgg
       61 acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggaag
      121 agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg
      181 acaaaatagt ttggaactag atttcactta tctggttaac taatctagct aacctgccgc
      241 tcagcttagt acgatcctat atccaactga tcataatcct ttcatcagta tcccaaatta
      301 aatccccctc ctagactgcc tcaccaacac tttacagtga tcccgtattt ccggacaggt
      361 gattgatttt tttcgcttgt acaggtgtta agccacagtt atcttgatgt ggacattatg
      421 cattgtaata gcccattaac atgcgctaag agcataaatt aatatacagt aacaagcatt
      481 acgaggtgct aatgtttgga aagcagcctc ttccaactac aataataagt taactatcct
      541 ccacccacaa ataattacaa atgacccctt tccgctataa ctccgatctc accagcggct
      601 cgctacaaac ccgagagtgt cgcattatta ctggcttgtt actccaagag cttgatgaag
      661 ctgcctggga taaagccatg tataaggaaa atgtgcttca gaaacgcacg caatctacgg
      721 taagacgtat ttcttcagcg cttagaaaac gcctagaaca cctaagctct gacttttggg
      781 cttttgcgtt tttatgttag gcattgtctg gcaactggag gaagtgtaat gaccatgaat
      841 ggtcactgtg attgacagta tcttgcttaa acttatctga acaatggtag aactggctca
      901 tatttatccc ctctatgatt gtttatcttt ttcaccataa aaagtatcag gggctactca
      961 gggctacata aagatattta tctactttta ctgattgata agtagcatca gtccatccgc
     1021 aggactggtg gtgccggcaa aatgctgacc caacacatgc gtatagattt gcgtggtctt
     1081 aacatcgtta tgccctaaga gttcttgcac agtgcgaata tcacgccccg cttgtaatag
     1141 atgcgtagca aacgagtgac gaaatgtatg acaagtgaca cgcttgctaa cgatgcctgc
     1201 tttttgtacg gctgccttca atgcctttcg cgcaacggag tcatgcagat gatggcggca
     1261 taatttgccg ttatacgggt ggttgcagag cgtgctggag ggaaagacaa acatccacgc
     1321 cgcttgtcga taagcagaag ggtatttgtg atctaaagca aaaggcagcg atggccctac
     1381 gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc tcaatgagtt attttattgc
     1441 tgggattagg cgcgtgggca gtaggctgtt tctgcttttc ccacccttac cgtcatgcac
     1501 agtgatgcag ccattatcaa aatcaaaatc tttaacccgc aaacgcaagc attcattaat
     1561 gcgcaaacct gcaccataca gcagcgtaaa aataacttgg ttgcgagtat ccataacctg
     1621 caaaatgcgt tgcacttcat ttgcagagat aacagagggt agccgtctag gcttgcttgc
     1681 agggatataa tcaatatcgc ccaacggctg ttgtaaaaac ctgttgtaca aaaaagctag
     1741 ggcatttaaa gcgattttct gcgtgtttat ggctacatgt ctgctgtttg ctaagctgga
     1801 taaaaacagc ctgacctctt cactgcccat ggtctgagga tgacgttttt tgtgaaacag
     1861 aataaaacgc ttaatccagt gcaggtaagt tttttcagtt ttcagcgcat aacctttttg
     1921 tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta gacataaaac gctccttgtc
     1981 ttgcaactgt ctgcctatac agcctattct agctgggatt taaaaaagtg cctgtttttt
     2041 acgcctagag atgcttgttt accggtagag ttttaattta atgctaaata aattaaaatg
     2101 ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc aggatagacg gcatgcacga
     2161 tttgtaataa cagagtgtct tgtattttta aagaaagtct atttaataca agtgattata
     2221 ttaattaacg gtaagcatca gcgggtgaca aaacgagcat gcttactaat aaaatgttaa
     2281 cctctgagga agaattgtga aactatcact aatggtagct atatcgaaga atggagttat
     2341 cgggaatggc cctgatattc catggagtgc caaaggtgaa cagctcctgt ttaaagctat
     2401 tacctataac caatggctgt tggttggacg caagactttt gaatcaatgg gagcattacc
     2461 caaccgaaag tatgcggtcg taacacgttc aagttttaca tctgacaatg agaacgtatt
     2521 gatctttcca tcaattaaag atgctttaac caacctaaag aaaataacgg atcatgtcat
     2581 tgtttcaggt ggtggggaga tatacaaaag cctgatcgat caagtagata cactacatat
     2641 atctacaata gacatcgagc cggaaggtga tgtttacttt cctgaaatcc ccagcaattt
     2701 taggccagtt tttacccaag acttcgcctc taacataaat tatagttacc aaatctggca
     2761 aaagggttaa caagtggcag caacggattc gcaaacctgt cacgcctttt gtaccaaaag
     2821 ccgcgccagg tttgcgatcc gctgtgccag gcgttaggcg tcatatgaag atttcggtga
     2881 tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt cgtgaagtgt
     2941 tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg agcccctacc
     3001 ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat ggcgcattca
     3061 tcgaccaaga gcttgtcggg aagattgaac tcaatctcaa catggaacga tctagcctct
     3121 atcgaacaca ttgttgtgtc gcacacgcac cgaggcaaag gagtcgcgca cagtctcatc
     3181 gaatttgcga aaaagtgggc actaagcaga cagctccttg gcatacgatt agagacacaa
     3241 acgaacaatg tacctgcctg caatttgtac gcaaaatgtg gctttactct cggcggcatt
     3301 gacctgttca cgtataaaac tagacctcaa gtctcgaacg aaacagcgat gtactggtac
     3361 tggttctcgg gagcacagga tgacgcctaa caattcattc aagccgacac cgcttcgcgg
     3421 cgcggcttaa ttcaggagtt agagagctgg ggaagactat gcgcgatctg ttgaaggtgg
     3481 ttctaagcct cgtacttgcg atggcatcgg ggcaggcact tgctgacctg ccaattgttt
     3541 tagtggatga agctcgtctt ccctatgact actccccatc caactacgac atttctccaa
     3601 gcaactacga caactccata agcaattacg acaatagtcc atcaaattac gacaactctg
     3661 agagcaacta cgataatagt tcatccaatt acgacaatag tcgcaacgga aatcgtaggc
     3721 ttatatatag cgcaaatggg tctcgcactt tcgccggcta ctacgtcatt gccaacaatg
     3781 ggacaacgaa cttcttttcc acatctggca aaaggatgtt ctacacccca aaaggggggc
     3841 gcggcgtcta tggcggcaaa gatgggagct tctgcggggc attggtcgtc ataaatggcc
     3901 aattttcgct tgccctgaca gataacggcc tgaagatcat gtatctaagc aactagcctg
     3961 ctctctaata aaatgttagg cctcaacatc tagtcgcaag ctgaggggaa ccactagtgt
     4021 catacgaacc tccaagagac ggttacacaa acgggtacat tgttgatgtc atgtatgaca
     4081 atcgcccaag taagtatcca gctgtgttca gaacgtacgt ccgaattcca gactatgtcg
     4141 aactagataa cgcggaggag ctggccgacg caatcattcg agaggttgag ttaaaggagg
     4201 catggctcac aagggtcttg gggaactcag ccgatcctga cgcttctgtg ggcaagtggg
     4261 accggcgcct agcgcagtgc ggtggtattg atgagatgtc aagtggggtg caggcaacat
     4321 acttcagtcc aaacggtcag ctggtcggct tagtggcata cattgatgcc gaatggatgg
     4381 atgaatagct ctgacgttac gattcaggtg agacaaccag atgggcattg cgtacaagct
     4441 cagttgccac cgatgtggct acgagtctga tcttctttac ctaggccaag gtatggcgat
     4501 gcttcctgag caaatagtcg gaacgtgcac atgctgcgac aatctaacga cgatttctat
     4561 aaatgaaagc tacggagtat gctcactttg tggggctaag gatcgtgtga tatttacaag
     4621 ctatcagcat gaggcaaggc caagaaatcc ctgctatcta gagagaagtt tcaaatatca
     4681 atgcccaaga tgccatgcct tcagtatgga gcctccggat ctaccggaga tgctttggga
     4741 ctaatgctcc gctttgcctc gggtatccgt tcgacctatg tttaagagga aacgcacgtg
     4801 tctgccgcca aagctcttgc ctatgcactt tgtgacaagt tggaaagcaa aaaagttgat
     4861 gaagtcgtac gaaatcttgt ttctagcggc tggaatatta gggaagtggc ctgggaagct
     4921 tcgtcctttg atcatgaaat gccctacttt gacacggggc gggacttcga tattggattt
     4981 cctgacacgc tgtcatccaa gattgagttt ttgaccgcga atggatcaat cgtccaagca
     5041 cgtgctcgcc tgcacttcaa gcaggctcta atttttgcca aggcgtccaa acatgtcgcc
     5101 gcgctcaaga actcattgga ttactactat ggatcgtctg taatcccccc tcaaaaccac
     5161 ggaggctatt tgtagaaaat tccattaata tgaactcaag gtgttcccta cggacgaatc
     5221 agcgatgaag gtggtgtacc tggctatcca ggcggcgacc aagaaatgga cgatgccgat
     5281 ccgcaactgg aagccggcca tgaaccgttt tatgatcgaa tttggtgacc gtttaaatgg
     5341 ccacctttaa ctgaccggca aaagcactta cacaaaaggg tttacaggct ctttcagtca
     5401 ttcctttaat gcgtaaattg ctctctaacc actctctcag cccacccttc catatcctct
     5461 ctcgatactt gcagctcccc caaccccttt ggatgattca cccctctatg cccgtcttgt
     5521 ccatataatt gatcaaacaa actatttggc caattcaatg agctcttcac cactccacgc
     5581 cgtatctttt caaaatcatt ccgccatgtt tcgctatcaa cgccactcaa cactttggta
     5641 gaaagcattt tcaccccatc cgatgcatcc acctctagca atacaaattc actgccatta
     5701 cgttgcaccc caacggcttt aatcactctt ggtgagccat ctttacacag atgtaatcgg
     5761 ctacgcccaa cttttggcaa aaccaaggtt tcttcaaaaa gcacccgaca tgcaaattta
     5821 gtttttagaa ttgaaagtag ctcatcaaaa gcagcaaatc gattagcaaa aatagagttg
     5881 taattggttg catcctgctt cccacccaca tctgccgctg ctaggacacc acctaagtgt
     5941 ggctcatcag tactaaccaa attactattt tcacttgttg ttgcttcttc tttttctaaa
     6001 atagtttggc tactttttct tgccctgcga gatttttcaa ttcgactagg tctactaaaa
     6061 cttatccatg tcggctcagc ctctatgact cgtgtgtctg tgtctatatt ggctgtttcc
     6121 tcatcgtcca actgtagatg ctcatcatca acaactgact ctgttgaaac cgctatgtgc
     6181 tgcgtactac catcacctgc ctccttttcc tgaaaagagg catggctaat agctgttgtg
     6241 ctaggaagca tagcgttgat ttctaaacct actatttcct caactaaata atccttatcc
     6301 tcgttagaag aacgtccttt tacatgtaat ttccaaccct gcatgggcgg agggtcaaag
     6361 ctaaagcacc agctttcaac gccgttctta atttctctat tttgttgata gtgcctaaaa
     6421 atactttcat acgaatccat aacatcttga tccgaaaaca accaaaccaa aagctgcact
     6481 acggccgact gctctaacgc ccctttagga aacgatgaac tgggtaagat ccttatctct
     6541 aaatgatctc gctcaacctc atactgaacg tcgaactctt gctgtaaagc ggtactgctc
     6601 aaacagcttc gacagaaata ggagttaatt aaaaataatg accgagctag ctctaactgt
     6661 gggatgtgat aacgagtctt gctatatggt gtttcagctt cgtaaataaa tgaactctgc
     6721 gctccatcct tgttgcgtac ggaagcaaag gacgagagat taggaaaatc gattattttg
     6781 gctcgttgaa gatccgatgc ttcaaactca atcacacgat ccgccttatt tatttgcttt
     6841 gttgtactgt taaccacctt agctcgactt aataaaggca aatgagtaaa tcgagtccat
     6901 ttccgttctt gcattggatt aaaccaaaca aaaatacgcc actccttatg acccgagtta
     6961 cggaataaat gaccaatatg tacaacctgc acattgtcat taaatgtagc tagcctaacc
     7021 actcactctt ccccataaac catttccaag aatctttgtg cttcctcagt tatccgctct
     7081 ttagataacg ttgcacttct taataatcgc cagcgcctaa gctcaacatc ttcctgttta
     7141 agcttaataa aagcttggct aatccgtcta atttgataat cttccacact ctctgagtaa
     7201 cgctttaagc aaagcgctac caaaggcagt ttctgtagat tttttgcaag agaggttccg
     7261 ttaggagttt gctttaacag ccagctcgat gtcgctcttg ggtgatcaag gctactatct
     7321 agacgcttta tgattcttaa tagttgtcgt acagcaattc gatctctttg gttccaatca
     7381 actctagggg cgggtgccag acgctcttgt tgatgctgtt gattccagtg aactagccaa
     7441 tccctgtcat gtcggtaaag ccaagcgtat agcaccccac cctctaaaga ctgtcttgcc
     7501 gccttaattc cttggtattt atgcactagt tgctgccagt ctttatgctt aacagataaa
     7561 tcttcagagt taggttgcac agactggcta acaggtcttg tcgttataga gtgctcagta
     7621 agagcacttg cctgctgtag cgcttctata accgttagtt ttggcaataa ggcttgccac
     7681 acaatactat gctgtaaata actaaaggct tttctatgct tacggaatat acttttcagc
     7741 caacacgtgt ccttgttctc tgccaacttt aaatccagtt tctctagtgc ctcatcacta
     7801 aaagtctgcc tcactctctc cgccaccaag tcatgacgaa tgtgcttgct tttggttagc
     7861 cctagatcct gcgctaagcg ctgataaaac agcgtccact gctcaaggct tggggaaagc
     7921 tcttgcgctc gtggagcatc taacagaggg gctatataag cagctagtgc tgttaattga
     7981 gatagggagt ctttggggta gtctgaaagc agctcagtat gacccaaagc ccaaaattga
     8041 tgtcggtgat catctacagc tctatcaaag aagactaaag caccgtgttt tggacaatat
     8101 ggcaaagcgg gcaaatacca atctcgttgc caaaaggctt ccccatacct atttagctga
     8161 agagcaacgc aatcagggca gtatctaaag cggttatcgc tcttaactct agaagcagcg
     8221 actcctagca ttaaatgcac cgcaccttgc gcttggtact ccattaaccg aatagcttcg
     8281 tctcggcgct ccttgcctac aaacggagca tataaaggga ataaggtatg ctcataaata
     8341 agctgctgaa cagcgtaacg tcctgtttga tgtagatgtc ttgctatcac acctaaatgc
     8401 gagggcagac ctaaggtagc gaccaccttg cggttgccat acacctcatc caacagctgc
     8461 ttaggactaa caatcccttg ataaacgcct gcccgtgcaa tagtgctata aatcagctca
     8521 ttcgagtacg gaacaggaaa gtttctcatg gctaacctgc ttgtttaaat aagctcgcca
     8581 tatccactat tacccctttc ccttttaacc gttcatgcat ggttttttca ggttggcgtt
     8641 gtgaatagat ataacgtaaa tccgtatcag gcaagctatc ccagtcgctg ggcttgacta
     8701 cctttatagc cgaaaccttt ttactcttgg agggcttttc tagttctgac actaccgttt
     8761 cgccttccat caaccactgc aaaacaagag gcagtaactt ttgtcttgtc atcgttggat
     8821 tctggctaaa cgctttttta atagtgggaa ttaacaggct tgaatcgtaa tcctctttca
     8881 gcatcagata taaatgacgc tgatcttcgg tatctaactc ttgaagggct ttttcttctg
     8941 gtgtttgttc ttgtatcgct gcgatatcta gctgaagttg gattaaccgt ttatcaatct
     9001 cgggaacgac tagatcagaa taacgagcaa tgcgttctgg gatacccgag cgtaatgcct
     9061 ctagcatggg gtgcacaggc tttaactcat cttgatacac ttgccgcaat aaaccagcgg
     9121 taatacgctc attgcctaaa gctagcgcac ggagctgagc gagtacaaaa agttttacta
     9181 caatgtccat cactccttgg cttagctcat accacacatc acggacctca tccgataaca
     9241 gcgcatcttt gcgttgtaaa agctgtaatt gccaaagatt atccgtaaaa gcgatccact
     9301 cttgattggg ctttccacgt tgcgtttgtt gtataggatc ccagaatata gctccaaacc
     9361 ctgcccctct acgtgcagac cgcaaatcag cctcaaaaat ctctcgtgct ttaggggtac
     9421 caatcaacat cactggtacg ccaataatat tcaccatcgt cacaaaaaag ttcagcatct
     9481 cttgagatcc acccgaacga gagcggctta aatgctgaat ttcatcaata accaacaacc
     9541 ctaaagcatg tgcattggct atttgcgaca tcaaagccaa catggtttct ataccatgac
     9601 gttttaagcc ataacgacgc tcatagttcg agcccaaggc tcgatccaac gctctgaaaa
     9661 aattcaagca gatttctttt agcgaaccat tatgcgagca gtctattttc aaatacacca
     9721 cctgctctac attgagttca cgatggtaaa tcacctgagg atacgtggct agaatacgat
     9781 gaagagaggt cgtcttccca ctaccagaac aaccaattaa caataagctt tgtgccgtag
     9841 atcgtgcctc ctcaaagcga aatgtctcca actctcccgt ttgaacacgc tcataaccat
     9901 tttgtaaatg cttttgtaaa tctcctgttt taggatttct gcctacgtag ccacctcgaa
     9961 tcatgaccga aatacgctca cttagtagca aatgcgtacc taatggctga aaatagtcat
    10021 ctggaatacg acaaatggta tgagctctga taacacggga cttttgcaag tcagaggaag
    10081 taagctgtaa agaggatttc agtgatgcag cactattcac tgactcttgt aatggtggta
    10141 aggcctcgat aaaaggatta tcacgataag cctctacccc cgtatcacga taaactgctt
    10201 gaatccgggt agcactcatg actcatcctt ttctggtgga tcctgaaata attcaggcac
    10261 gtatgtgggt aggctgtaat cttcttgatc atccgcttcc actgcgttga aaggaataac
    10321 tttagcctca tcacctgaag agcttggctt caaatgctcc gcacgttttt tacgctccga
    10381 ggtcacggct tcttttttat tagttttaat ctgcttaatg cgtgttgatt tgggctcagt
    10441 agtactgggc gttaacttat tcgctttctg aatggtttgc tgaataaacg cctcaagctc
    10501 cctgcgttta gttaactcat cctgcttcgc attggcttta ttgtgttttt cttgtgcttg
    10561 tatatcccaa acctcccaaa atgagagacc tttaaactgc cgactacgtt ccgtcagatt
    10621 acagcgccaa aatacacggc tgccaacttg cggaaacaaa taaatcgtat caaccagcaa
    10681 tgggtcataa gccgcttcta aatgttgagg tctagctata tcagtgctcc gctgcaacca
    10741 accctcacgc agaatctctg accccgagta atacaaaccc cacaaattaa cgccaaatga
    10801 agaaatagag acctttcggc gaggcagtaa cgctactcgc aactgctctt gctccacagc
    10861 ccttaaacta cctgtacgat gctgcatacc ccattgccat agctggacag gaatagacgg
    10921 taaatctgta ggaaaatcag catctcgatc gtatttatcc atcaccagat ggttatttct
    10981 gaataagatc gtacgcaaaa taatttgtgt gaactcaaat accgacagag atgcatctaa
    11041 cctatagtct gtttcaccat ggcttttgat ccgactgccc tctacaatgc caggtgcaaa
    11101 ggacttaaac tcggcttgta gtgttctaaa agtgctttcc actatgcctt tagcatcgcc
    11161 acgtctaggt ggagcacttt ccactcgcac attaaaacta gaaactaagg cttcgacctg
    11221 atgactcatt aattcgccac ggtccgctag caacacatct ggcaaaccta cacacggcca
    11281 gtctgagcta ctaatctcaa tatcatgctg ggcacaaatg gccgttttgt cagagcaagc
    11341 atttacaaaa gcctgcatcg ccaccacata agacggattt tcaaagccga tataaaagcc
    11401 cgtgatcatc cgactaaaca catcaatcac aatgtaaagc gttggtcttc ctatgatttt
    11461 ttggcgatca tgatgatcca ctaaataaat atcagcaatc gtggcatcaa tctcataacg
    11521 actcccaggg cctaacgcct gagaagtggc tgtactactt aagggtcgta cgtctttttt
    11581 atatacccct gctttaactc tagactttaa gcgctgagct ttagggtatt ctcgatcata
    11641 aaaataacga aactgacgta gtgttgggta atcctcttgg ggaatgcgag gaaaatactg
    11701 agcaaacaag tccacaaatc gtctataggc aacggtggtc tttgtacctt tttgatttaa
    11761 caggtgcttt tctatggtca acctaaaaag gcgttcaatc tcgggcgtta ccttggttcc
    11821 ttcaccctta ccatattctc tggctcggcc aatctttgct gttcctgtcg ctgaacgtct
    11881 ttcccctggt gcaccgctgt ttttgtagtc aggaattaat gcattaggcg tttgaccacg
    11941 ctgccagtaa cggcgtaaca acttataaac tgtagcctta gtaaccttat gttcttggac
    12001 cacatgctca acgagttcgc ttctgacttt agggtcgaaa cgatccttac tattaataat
    12061 aggaagaatt tttcgataat cctcctcgcg cttctggaag ctgacagaat ctacagacgg
    12121 ctcttctaaa tcaagatgta cataagggtc atcagttcta actaagcggc cttcatctaa
    12181 atactgcatt agcaacaact cagccctagc ttgtggaact cctttatcag cgcttatttg
    12241 catccagaca acttggccat cctctatagc caaaatgcga tacggatcat tatcaaatag
    12301 cacaacctca ttaatttgcc acatagcgca actcctccat attcactact tggctaatac
    12361 agagatctgc acacttattt gccctgaaag acttataaat attgaactta ataaaaccat
    12421 ttgcggttaa ggctcgtatc tcactcaatg ttttgcctaa ctccaaatca taagcaatat
    12481 caacctgctt acagacattg ataatgtttt catctccttt ttcttgcagg atatgggcca
    12541 atggggatag ttgtgctaaa agctccgcag aaacttcttc tgttttcact gaataaagcc
    12601 attcaatatt ttcttttact acgggattta tttctttatc agtaaaaatg aaccaaggaa
    12661 tttgcttttg ctgccaatag cgacgctcta gttctagttt ttctaaggta cgctcgtctt
    12721 gtaaggctgc tgcaggtttg acttgaatag caaactgctc aaaaggacca tctttgcagt
    12781 ccactaaaaa atcagtagac ataacctgat ctacaccacg aataacagga tgcttaatac
    12841 cactatctat tgcaatctgc ctggtatcac taggtaataa ggggaactgc tcgcgtatat
    12901 ctagcacgct gctctcccac tcaagactga gaaaaacagc aagctctaag tcagatagca
    12961 aatgatggac tcgtcccgtc ttatgagaat aaatacggtg ggaacgacct gaagaaggaa
    13021 cttcttgtac tgttagccat ggaatatagt ctttaccatg cccttggcca cgcccctctt
    13081 taatacgacg ggcaatttgc acttcagaaa atgaagagtt tgctttagcc ataacaaaag
    13141 tccagtatgc tttttcacag cataactgga ctgatttcag tttacaacta ttctgtctag
    13201 tttaagactt tattgtcata gtttagatct attttgttca gtttaagact ttattgtccg
    13261 cccacaagcc cttttttatc tatgatagtg attactcaac cgtaaccgat tttgccaagt
    13321 tacgcggctg gtcaacgtcg gtgcctttga tcagcgcgac atggtaagcc agcagctgca
    13381 gcggaacggt gtagaagatc ggtgcaatca cctcttccac atgcggcatc tcgatgatgt
    13441 gcatgttatc gctacttaca aaacccgcat cctgatcggc gaagacatac aactgaccgc
    13501 cacgcgcgcg aacttcttca atgttggatt tcagtttttc caacagttcg ttgttcggtg
    13561 caacgacgat aaccggcata tccgcgtcaa tcagcgccag cgggccgtgt ttcagctcgc
    13621 cagcagcgta ggcttcagcg tgaatgtagg agatctcttt cagcttcaat gcgccttcca
    13681 gtgcgattgg gtactgatcg ccacggccca ggaacagtgc gtgatgtttg tcagagaaat
    13741 cttctgccag agcttcaatg cgtttgtcct gagatagcat ctgctcaata cggctcggca
    13801 gcgcctgcag accatgcaca atgtcatgtt caatggaggc atccagacct ttcaggcgag
    13861 acagcttcgc caccagcatc aacagctcag ttaactgagt ggtgaatgct ttagtggacg
    13921 ccacgccgat ttccgtaccc gcgttggtca tcagcgccag atcggattcg cgcaccagag
    13981 aagaacccgg aacgttacag attgccagcg aaccaaggta acccagctct ttcgacagac
    14041 gcaggcccgc cagggtatcc gcggtttcgc cagactgtga caaggtgatc atcaggctgt
    14101 tacgacgcac ggcagattta cgatagcgga attcagaggc gatttcgacg tcgcacggaa
    14161 tacctgccag cgattcaaac cagtagcggg aaaccatacc ggagttataa gaagtaccac
    14221 aggcgaggat ctgaatatgc tcaaccttcg acagcagttc gtcggcgttc ggtcccagct
    14281 cgcttaaatc aacctgaccg tggccgatgc gcccggtaag ggtgtttttg atcgcgttcg
    14341 gctgttcgta gatctctttc tgcatgtagt gacggtaaat acctttatcg cccgcgtcat
    14401 attgcagatt ggattcgata tcctgacgtt ttacttccgc gccagtttta tcgaagatgt
    14461 ttaccgaacg gcgagtaatt tccgcaatat cgccctcttc aaggaagata aagcgacggg
    14521 tcaccggcag cagcgccagc tggtcagaag cgataaagtt ttcgcccata cccaggccaa
    14581 tcaccagcgg actaccagaa cgtgccgcca gcagggtatc cgggtgacgg gagtccatga
    14641 tcactgtacc gtacgcaccg cgcagctgcg ggatagcacg cagaacagcc tcacgcagag
    14701 tcccgccttg tttcagctcc cagttcacca gatgggcaat cacttcggta tcggtttcag
    14761 aaacgaaggt atagccacgc gcttttaact cttcacgcag cggttcatgg ttttcgatga
    14821 tgccgttatg caccaccata atgtgttcag aaacatgcgg atgcgcgtta gcttctgaag
    14881 gttcaccgtg ggtcgcccag cgagtgtgag caataccggt gccgccatgc agaggatgtt
    14941 cttccgctgc ctgagccagc atctggactt taccgaggcg acgcaggcgg gtcatatgac
    15001 cttccgcatc aacaacggcc agaccggcag agtcatatcc gcggtattcc agacgacgta
    15061 aaccttcaag aaggatttct gctacatcac gttgcgcgat cgcgccaaca attccacaca
    15121 tagtttttga ttccgattta tatcgttgtc ggtcaacctg tatgcccgtt ttttcgggcg
    15181 ccccgagcct tgtagagagt ggggttattt ttatagttac tgcttgtggg agggagatta
    15241 tgttatctcc tcatcccaga tgaccggatt acccggccag aattactttt tctttaccgg
    15301 acgacgccag ccttctttct gagtctgcgg cacacggctg atagctaatg cattttcgcc
    15361 gacattacgc gtcacagttg tacccgcagc aatggtcgcg cctttgccta ctgttaccgg
    15421 ggccaccagc tgagtgtcgg aaccgacaaa cacatcgtcg ccgataatgg tcttaaattt
    15481 attcgcacca tcgtagttgc aggtaatggt tcccgcgccg atgttaacgt tatcgccaat
    15541 ttccgcatcg cccaggtaag tcagatgacc agctttcgag cctttaccca gacgcgcttt
    15601 tttcatctca acaaagttac cgacgtgtgc accttccagc aactcagcac caggacgcag
    15661 acgggcaaac gggccaatag tacaggccgc tgccagattc acgtcttcca caacggtata
    15721 cggactgatt tcgcaatcat cgccaatcac gctgttttta atcacgcaac cggtgccgat
    15781 tttcacgcga tgaccgagag tcacgttgcc ctcgatgata acgttagtat caatttcaac
    15841 atcgcgcccg tgagtaagcg taccgcgcag atcaaaacgc gccggatcgc gcagcataac
    15901 gcctgctaac agcagttttt cagcctgttc ggactggtaa acgcgctcca gacgggagag
    15961 ttgcaggcgg ttattcacac cttctacttc gcttaaacgt tgcggatgaa cggcgacgat
    16021 ttcacgccct tcctgatacg ccagcgcaat aatgtcggtg atgtagtatt cgccctgagc
    16081 attattgttg gtcagcttcg ccagccagcg tttcatatct gcgccgttag cgatcagaat
    16141 gccggtgttg atctcctgaa tctgacgctg ctcgtcggtg gcatctttgt gctcaacaat
    16201 gccggtaact ttgccatttt cacgggtgat acgtccataa ccggtcggat catccagttt
    16261 caccgtcagc agaccaatgc caccctgcgg tttagcatca cgcaggcgct ggagtgtttc
    16321 gacagagatc agcggcacgt cgccgtagaa cattaaaatg tcttcatcat cggcaaagaa
    16381 aggtgcggcc tgctgcattg catgacccgt acccagctgc tctgcctgaa gcacccagtt
    16441 aaggttgtcg tctttcagcg cctgtttaag cagatcgccg ccgtgaccgt acaccaggtg
    16501 aacgtgcgct gcgcctaatt cattcgcagc atcaatgaca tgctgaacca tcgctttccc
    16561 ggcaagggta tgcagcactt tcggaagatc ggaatacatg cgcgtgcctt tgcctgcggc
    16621 aaggatcact acgctcatag cattattcaa catacgcgtc ctgactgtaa tttgagaacg
    16681 aatttaaacc gcttcacctt gaaaaaacta catttttttc atcgtgaaat ggacagagga
    16741 taaattgttc aataacggat tatccccgct gacgaaagtg ccattttcga ccatcgtcac
    16801 gccttttgtc tgcagaaaat taaggcagta ccttcattta agcgtcaggt ggatgttttt
    16861 gctcttattt cgatcaatga ataaacggga aaacaggggt gttttatatc agcaggatct
    16921 atgtgaacgc tcttcaggac ggatcacgcg taaaaaaaac cagcctgttt ccagactggc
    16981 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
    17041 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
    17101 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
    17161 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc agactgcact tcaagaatgc
    17221 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
    17281 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
    17341 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
    17401 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
    17461 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
    17521 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
    17581 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
    17641 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc
    17701 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
    17761 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
    17821 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
    17881 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
    17941 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
    18001 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
    18061 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
    18121 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
    18181 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
    18241 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
    18301 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
    18361 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
    18421 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
    18481 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
    18541 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
    18601 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
    18661 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
    18721 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
    18781 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
    18841 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
    18901 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
    18961 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
    19021 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
    19081 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
    19141 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
    19201 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc
    19261 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
    19321 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
    19381 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg
    19441 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
    19501 tcgcggtctt ccaggtaagg gtgcttatat tccagattac catgtgcaag gtgaccaatc
    19561 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
    19621 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
    19681 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
    19741 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
    19801 tgcctttcag tttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
    19861 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
    19921 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
    19981 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
    20041 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcta
    20101 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
    20161 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
    20221 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
    20281 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
    20341 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
    20401 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
    20461 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
    20521 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
    20581 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
    20641 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
    20701 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcacgc tggttgatga
    20761 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
    20821 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
    20881 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
    20941 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
    21001 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
    21061 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg
    21121 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
    21181 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
    21241 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
    21301 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
    21361 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
    21421 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
    21481 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
    21541 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
    21601 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
    21661 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
    21721 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
    21781 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
    21841 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
    21901 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
    21961 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
    22021 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
    22081 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
    22141 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatatg ctcgttctgc
    22201 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
    22261 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
    22321 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
    22381 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
    22441 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
    22501 gaatcagatc aggttgacgc gctgcgcctt ccaagaattt acccccgagg atgccgatac
    22561 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
    22621 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
    22681 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
    22741 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
    22801 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
    22861 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
    22921 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
    22981 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccaat
    23041 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
    23101 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
    23161 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
    23221 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    23281 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    23341 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    23401 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    23461 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    23521 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    23581 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    23641 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    23701 gcgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    23761 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    23821 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    23881 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    23941 ctttctttaa ggcttagagt caagcatcaa aaagaggtca aattatacgg cgcgcccccg
    24001 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    24061 acaattgcga cttttctgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    24121 cactggtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta
    24181 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cactctaaaa acagatactg
    24241 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    24301 gcagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    24361 attttctgct taccaaaaaa agccacgtta tcttgttgat gcaaaagagt gaacgtggcg
    24421 ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt gttaacagta taaccggtca
    24481 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    24541 cgttcgccat ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    24601 ggcaacaaag cgatttcatc ttccggcctt tgccctttca gcgcgtagaa acggccttgc
    24661 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    24721 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggttca
    24781 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    24841 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    24901 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    24961 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    25021 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
    25081 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    25141 tgttcttgat gcgttgcctg gtaagcgggt gcttaccaga catttttaat gcgttatgcg
    25201 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc
    25261 gtgacgccag aaatacgcga agcttggccg atagaggctg gtttgtgatc gttaagtttg
    25321 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    25381 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    25441 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    25501 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    25561 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    25621 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    25681 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    25741 aaacgcagat ccgcattatc ttcgcgtagc atcagacgat attctgcgcg cgaagtaaac
    25801 atacgatacg gttctttggt tcctaaagtg cacaggtcat caactagtac gccgagatac
    25861 gcctgagaac gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    25921 ccggccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    25981 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg
    26041 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    26101 atagagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    26161 taaatttcat tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtcg
    26221 gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc
    26281 acccctgcat acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    26341 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
    26401 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
    26461 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
    26521 cgcaaacggt gagaaagcgg aatggacggc ggatcaccag cacggccacc gctgtaatta
    26581 tccagaccga tatgaa
//
LOCUS       DAJTNA010000486         9617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.guided.144, whole genome shotgun sequence.
ACCESSION   DAJTNA010000486 DAJTNA010000000
VERSION     DAJTNA010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.guided.144"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(38..604)
                     /locus_tag="OO161_004490"
                     /pseudo
     CDS             complement(38..604)
                     /locus_tag="OO161_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(823..1800)
                     /gene="epmA"
                     /locus_tag="OO161_004491"
     CDS             complement(823..1800)
                     /gene="epmA"
                     /locus_tag="OO161_004491"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5465243.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGSETLAEVIAFSVDRA"
     gene            2125..3933
                     /gene="frdA"
                     /locus_tag="OO161_004492"
     CDS             2125..3933
                     /gene="frdA"
                     /locus_tag="OO161_004492"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5465244.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVPIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3926..4660
                     /gene="frdB"
                     /locus_tag="OO161_004493"
     CDS             3926..4660
                     /gene="frdB"
                     /locus_tag="OO161_004493"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5465245.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIEHD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4671..5066
                     /gene="frdC"
                     /locus_tag="OO161_004494"
     CDS             4671..5066
                     /gene="frdC"
                     /locus_tag="OO161_004494"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5465246.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5077..5436
                     /gene="frdD"
                     /locus_tag="OO161_004495"
     CDS             5077..5436
                     /gene="frdD"
                     /locus_tag="OO161_004495"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5465247.1"
                     /translation="MINPNPKRSGEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5499..6629
                     /locus_tag="OO161_004496"
     CDS             5499..6629
                     /locus_tag="OO161_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5465248.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAACQILNALQ"
     gene            6718..7251
                     /gene="blc"
                     /locus_tag="OO161_004497"
     CDS             6718..7251
                     /gene="blc"
                     /locus_tag="OO161_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5465249.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7248..7565)
                     /gene="sugE"
                     /locus_tag="OO161_004498"
     CDS             complement(7248..7565)
                     /gene="sugE"
                     /locus_tag="OO161_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5465250.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7747..7893)
                     /gene="ecnB"
                     /locus_tag="OO161_004499"
     CDS             complement(7747..7893)
                     /gene="ecnB"
                     /locus_tag="OO161_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5465251.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(8004..8129)
                     /gene="ecnA"
                     /locus_tag="OO161_004500"
     CDS             complement(8004..8129)
                     /gene="ecnA"
                     /locus_tag="OO161_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5465252.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8181..8747)
                     /gene="efp"
                     /locus_tag="OO161_004501"
     CDS             complement(8181..8747)
                     /gene="efp"
                     /locus_tag="OO161_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5465253.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8789..>9617
                     /gene="epmB"
                     /locus_tag="OO161_004502"
     CDS             8789..>9617
                     /gene="epmB"
                     /locus_tag="OO161_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5465254.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     "
BASE COUNT         2469 a         2492 c         2426 g         2230 t
ORIGIN      
        1 catcatacac taaatcagta agttggcagc attacccgaa atgcccacca tttaataaca
       61 aaatagtaat gcttactaac agcaatacta aattcagaca cattactgca ataccgccca
      121 ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc aacgacggtg accagaatca
      181 tccatgctac tgccagcaga ccaaccacct gcgtaggctc cagtccggca atacgccagt
      241 gctgggtcat gtcgctacca tagaggaatg ttgagaacgg tacccaaact ttcgcgtagg
      301 tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg aaggcaaaac
      361 gtggtccgac actattattc atccaggaat agataccacc ttcttctttg cgataagcag
      421 ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca gaaaatatat
      481 aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg gcaaatccaa
      541 atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt atcgtgtgag
      601 gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg
      661 caacattgag caggcagcgt aaaaaccaat acagaattga tactcggatg atatcaaata
      721 tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca gaatggcgct
      781 tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc ggtcaacgct
      841 aaaggcgatg acttcagcca gtgtctccga gcccagcgcc aacatcacca gacgatcaac
      901 acctaatgcc acgccggaac agtcaggcat accgactttc aaggcttcaa tcagattctg
      961 gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg
     1021 ttgttgctgc tcacgggcat ccgttaattc atggaaacca ttcgccagct caataccttt
     1081 ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt gcgccagtga
     1141 tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttgccaa tatttggctc
     1201 tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg tcttcttcgg tatcagcaac
     1261 gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg
     1321 gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg ctgccgggca
     1381 gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca tatcatagtg
     1441 cggtcgatac cactcaagca tagtgaactc agggttgtga taacgcccca tctcttcatt
     1501 acggaagctg cggcacagct ggaataccgg cccacaaccg gcaaccagca ggcgtttcat
     1561 atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc cggggcctac
     1621 gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc tcatacaagg
     1681 cgtctccacc tccagcactc cacgatcagc aaagaaacga cggatctccg ccataatcgc
     1741 cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg tttcgctcat
     1801 agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga cggcgagaca
     1861 aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg atctgatggt
     1921 ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac taaaaaaatc
     1981 gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat aaaggagcag
     2041 ttgaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg gggctgtggg
     2101 ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc
     2161 gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa
     2221 atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc agaagggggc
     2281 tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga tacagtagcg
     2341 ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca ctgcccaacc
     2401 gaaatgaccc aactggaact gtgggggtgc ccatggagcc gtcgcccgga tggtagcgtc
     2461 aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc cgataagacc
     2521 ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca gatccagcgt
     2581 tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg cggcctggta
     2641 gcaatgaaca tgatggaagg cacgctggtg ccgatccgtg ctaacgcggt cgttatggct
     2701 actggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt taccggtgac
     2761 ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat
     2821 cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc
     2881 ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat gggcccggaa
     2941 actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga caaagtttct
     3001 caggccttct ggcacgaatg gcgtaaaggc aacaccatct ccacgccgcg tggcgatgtg
     3061 gtttatctcg acttgcgtca cctcggcgag aaaaaactgc atgaacgtct gccgttcatc
     3121 tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat tccggtacgt
     3181 ccgaccgcac actacaccat gggcggtatc gaaaccgatc agaactgtga aacccgcatt
     3241 aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg
     3301 ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga acaagcgaca
     3361 gagcgtgcag caactgccgg taatggcaac gaagaggcaa ttgaagcgca ggcagctggc
     3421 gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg ggcgaagatc
     3481 cgcgacgaaa tgggcctagc aatggaagaa ggttgcggta tctaccgtac gccggaactg
     3541 atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc
     3601 accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact gggccacggt
     3661 ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca
     3721 caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaacttcct caaacacacc
     3781 ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt gaagattact
     3841 acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga taaggcggaa
     3901 gcagccaata agaaggagaa ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt
     3961 gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga
     4021 cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg caccggacct
     4081 gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga tggttaacaa
     4141 cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga
     4201 agcgttagct aacttcccga ttgaacacga tctggtggtc gatatgaccc acttcatcga
     4261 aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg atcagggtac
     4321 taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt gcatcaactg
     4381 tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca tcggtccggc
     4441 tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg
     4501 tatggcgcag ttgaacagcc agaacggcgt atggagctgt actttcgtgg gctattgctc
     4561 cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag
     4621 ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac atgacgacta
     4681 aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc
     4741 gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga
     4801 ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt
     4861 tacaaaaccc ggttatcgtg atcattaacc tgatcactct ggcggcagct ctgctgcaca
     4921 ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa
     4981 tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc accatcgtaa
     5041 tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt
     5101 tctggcgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag cgcaattatt
     5161 gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt tccgggtgat
     5221 gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg
     5281 ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac
     5341 gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct acggtctggc tgctatcctg
     5401 acagttgtca cgctgattgg tatcgttaca atctaacgta tcgccaatgt aaatccggcc
     5461 cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg acgctctgcg
     5521 ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg
     5581 tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg
     5641 taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc
     5701 agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg
     5761 tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat
     5821 actggcctga acttaccgct aaacagtgga atgggatcac actattacat cttgcgacct
     5881 acaccgctgg cggcctgcca ttgcagatgc cggatgaagt gaaatcctca agcgacttgc
     5941 tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt ctgtatgcca
     6001 actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc
     6061 aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac
     6121 cgcccgcaga agaaaagaat tacgcctggg gatatcgcga aggcaaggca gttcatgttt
     6181 cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa gatatggccc
     6241 gctgggtgca aagcaattta accccccttg atatcaatga gaaaacgctt caacaaggga
     6301 tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg
     6361 aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac aataaaattg
     6421 cactggcagc acgcccggta aaagcgatta agcccccaac tcctgcagta cgcgcatcat
     6481 gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa
     6541 aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg
     6601 cctgtcagat tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt
     6661 tctgcgcttt tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg
     6721 cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct
     6781 cctacgccgc cgcgtggcgt gacagtagtg aataatttcg acgctaaacg ctatctcggt
     6841 acctggtatg agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc
     6901 gcaacataca gtctgcgtga tggcggcggc ctgaatgtca tcaataaagg ctataaccct
     6961 gacagagaaa tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaacacgc
     7021 gctgcgctga aagtgtcatt ctttggtcct ttctatggcg gttataacgt cattgcactt
     7081 gatcgggaat accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc
     7141 tcccgcacgc caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg
     7201 gaagggtttg atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg
     7261 agtttcagac caataatccc caatacgatt aacgccagac tcgccaggcg catcgggtta
     7321 gcggactcac cgagcagcac aatgccggtg atagccgcgc cgactgcgcc aatacccgtc
     7381 cacacggcat aagccgtccc tactggtaac gatttcatcg cccaggcaag tagcgccata
     7441 ctgacaatca tcgccgtcac ggtaataaca ctcggcgtca aacgactaaa gccgtgggta
     7501 tatttcaggc caacggccca taccacttcc agcagaccag caataactaa gataatccag
     7561 gacatatcag gctccggaga attggggccg tccccggtga aaagacgcgt ttgcaggtcg
     7621 tcctgcaacg ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt
     7681 accttagtga ctatcgcaga aaaaacaaac ggcacgacac agctgtcgta ccgttattgc
     7741 ttattgctat tgctgcgctt tcgttgctgc gccagaaatc gcgttaccgc catcagaaat
     7801 gtcttcacca acgccacgcg tggtgttgca ggcagttaat actgttgaaa gcaccagaac
     7861 agaaaagatc gctgcaattg tcttcttcac cataacgtct tccttttagg taatgttatt
     7921 tatgtttgcc tatagcaaat taataataga caacatcgcc aaaaatgacg gaaatctgat
     7981 gatttttcgg aagagaagaa aaattagctg gcagcgcgag agatggagtt gccgagatgt
     8041 ttgatgtctt cgccgaaacc gcgagcggtg ttacagcccg tgagcagcgt actggcaagc
     8101 aaaacaagaa cgataaggcg tttcatcatc tctgacctga ataagtgatg gtgcagcctg
     8161 caggccgcac cacaaccgca ttacttcacg cgagagacgt attcaccaga gcgggtatcc
     8221 actttgatga cttcgccgat ttgtacgaac agcggaacct taaccacagc gccagtagac
     8281 agggtagccg gtttgccgcc agtacctgcg gtatcacctt tcaggcccgg atcggtatca
     8341 acgatttcca gttcaacgaa gttcggcgga gtaacggaga tcggctgacc attccacaga
     8401 gttacgatac actctgcctg atccagcagc catttagcgt tatcgccaat tgcttttgca
     8461 tcagcagaca gctgctcgaa agtttcgttg ttcatgaagt gccagaactc accgtcgttg
     8521 tacaggtaag tcaggttcat atcgacaaca tcagcgcctt cagcggaatc agtagatttg
     8581 aaggtttttt ctacgcgagt accggtcagc agacgacgca gtttaacgcg agcaaatgcc
     8641 tggcctttac ccggttttac gaattcactc gcttcaaccg cgtaaggttc gccgtctaac
     8701 atgattttaa gaccagcacg aaaatcgttg ctatagtacg ttgccataag gccctctgaa
     8761 atttgttaat tggtagctaa gccacaaaat ggcgcatatt gtaaccctaa ataccccatc
     8821 cagagaagat tggttaacgc aacttgccga tgttgtgacc gatcctgatg aacttctgcg
     8881 tcttttgaat atagacgcgg acgaaaaact gttggccgga cgcagcgcca aaaagctgtt
     8941 tgccctgcgt gtgccccgct catttatcga tcgcatggag aaaggcaatc cggacgatcc
     9001 tcttttgcgt caggtactta cctcgcaaga tgagtttgtc gtcgcgtccg gattctccac
     9061 cgacccactg gaagaacagc acagcgtagt gcctggtttg ttgcataaat accacaaccg
     9121 ggcgcttttg ctggtcaaag gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca
     9181 cttcccctat gccgaaaatc agggcaacaa gcgtaactgg cagactgcac ttgagtatgt
     9241 tgctgcgcat ccggaactgg acgagatgat tttctccggc ggcgatccgc tgatggcgaa
     9301 agatcacgag ctggactggt tgctcacaca actggaagcc atcccgcata tcaaacgtct
     9361 gcggattcac agccgtctgc cgattgtgat cccggcacgc atcacagatg cgctggttga
     9421 acgcttttca cattctacgc tgcaaatcct gctggtgaat cacatcaacc atgccaatga
     9481 gatagatgaa acattccgtc aggcgatggc taagttgcgc cgggtaggcg ttactttgct
     9541 gaaccagagc gttctgttac gtgatgtgaa cgataacgca caaacgctgg caaacctgag
     9601 taatgcgttg ttcgatg
//
LOCUS       DAJTNA010000487         6637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.guided.200, whole genome shotgun sequence.
ACCESSION   DAJTNA010000487 DAJTNA010000000
VERSION     DAJTNA010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.guided.200"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            complement(<1..2321)
                     /locus_tag="OO161_004503"
     CDS             complement(<1..2321)
                     /locus_tag="OO161_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5465255.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAP"
     gene            complement(2324..2884)
                     /locus_tag="OO161_004504"
     CDS             complement(2324..2884)
                     /locus_tag="OO161_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5465256.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(3010..3360)
                     /locus_tag="OO161_004505"
     CDS             complement(3010..3360)
                     /locus_tag="OO161_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5465257.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(3563..4576)
                     /gene="intI1"
                     /locus_tag="OO161_004506"
     CDS             complement(3563..4576)
                     /gene="intI1"
                     /locus_tag="OO161_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5465258.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            4786..5616
                     /locus_tag="OO161_004507"
     CDS             4786..5616
                     /locus_tag="OO161_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5465259.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            5729..6520
                     /gene="aadA1"
                     /locus_tag="OO161_004508"
     CDS             5729..6520
                     /gene="aadA1"
                     /locus_tag="OO161_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5465260.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1485 a         1810 c         1913 g         1429 t
ORIGIN      
        1 ggcgcgaagc ggaagcccaa gaggtgcatc agggcgaaga cgtgatcggt gaagcccgcc
       61 gtgtcggtgt agtgctcctc gatccgcagg tcggattcgt ggtacagcag gccgtcgagc
      121 acgtaggttg agtcgcgcag gccgacattg accaccttgg tgtggaatgg cgcgtattgg
      181 tcggagatgt gggtgtagaa agtccgtcct gggctgctgc catattttgg gttgatgtgc
      241 cccgtgctct ttgccttgct agcggttcgg aaattctgtc cgtccgatga tgatgtggtg
      301 ccatcgcccc agtgcccggc aaagggatgc cgaaactgag cgttgaccag ttcagccaac
      361 gctgtcgagt acgtttcgtc gcgggtatgc caggcttgca gccaagcgag cttcgcgtag
      421 gtcgtgccgg ggcaggactc ggccatcttg gtcaggccca ggttgatcgc gtcggccagg
      481 atcgtggtca acaacaggtt cttgtccttg gccagatcgc ccgatttcaa gtgcgtgaag
      541 tgccgggtga agcccgtcca ctcatcgact tcgagcagca gttcggtgat cttgacgtgc
      601 ggcaggacca tggctgtctg gtctatcagc gcctgcgcgg tgtcgggcac cgccgcatcc
      661 agcggcgtga tcttcaagcc cgactcggtg atgatggcat ccggcaggtc gttggctgcc
      721 gccatgcggt tgacggtggc aagttgtgct tccagcagcg tcagccgctc atgcagatat
      781 tgttcgcagt cggtggccac ggccagcggc aattcgctgg actgcttgag gctggtgaac
      841 ttctcgggcg gtaccaggta gtcctcgaag tccttgaact ggcgtgaacc ctgcacccag
      901 atgtcgcccg agcgcaggga gttcttcaac tcggacagcg cgcacagttc gtagtagcgc
      961 cggtcgatgc cggcgtcggt catcaccagt ttctgccagc gcggcttgat gaagccggtc
     1021 ggtgcatcgg ctggcagctt gcgggcgttg tcggtgttca tgccgcgcag cacctcaatg
     1081 gcatcaagca cgtttttggc ggcgggcgcg gcccgcagct tgagcacggc aaggaattcc
     1141 ggtgcatagc ggcgcagggt ggcgtagctc tcgccgatgc gatgcaggaa atcgaagtca
     1201 tcgggttgcg cgagcttctg cgcctcggtg acgctctcgg caaaggaatc ccaggacatg
     1261 acggcctcga tggcggcaaa cgcatcgcgg cctgattgct tggcgtcgat cagcgcctga
     1321 ccgatgcgcc cgtacagacg taccttggcg ttgatggcct tgcctgacgc ctggaactgc
     1381 tgctgatgct tattcttggc agcgttaaac agcttaccca ggatgcggtc gtgcaggtcg
     1441 atgatttcgt cggtgacggt ggccatgccc tcggtggcca gcgccacgag agtggcgtag
     1501 cgccgttgcg gctcgaattt ggccaggtcg gcgggtgtca tctggccgcc ctcgcgggca
     1561 atcttgagca ggcggttctg gtgaaccagc cgctcgatgc cggtaggcag atcgagtgcc
     1621 tgccatgcct tgaggcgttc gatgtgttcc agcatatgcc gcgaatttgg cttggccgga
     1681 gactggcgca accaagccaa ccaggtcgtc ttgccgttgt cccggcgctt gagcagatcg
     1741 tcgaggcggc ggcgatgcgc gtccgccagt ggttcggcca aggcgtcgta gatgcgccgg
     1801 ttagcacggg tgatcgcctc ggcactcgcc cgctcgacgg cgttgagggc gggcagaatg
     1861 accgactgcc gccgcaggtg cccgatcaag gcgctggcca gcacgatgcc tttgtcggtt
     1921 tgcatcgcca gctcggtcag catctggacg gcctgccggt aatggctcat ggtgaagggc
     1981 cggaaaccga acacggtttg cagctcgctc aggtgctcgc gccgggtctg ctcccgctgg
     2041 ccgtactcgt tccagctttc gacgccgacc ttgagctggt cggcgaccag cttcaacaag
     2101 ggcgggaacg gtagttcatc gacgcccagg atgacgccgg gaaagcgcag gtaacagagc
     2161 tgcaccgcga agcccagccg attggctggc ccgcgccgct gtcggatgat cgagaggtcg
     2221 gtatcgttga atgtgtaatg tcggatcagg tcgtccttgg agtccggcaa cgccagcagg
     2281 ctttcccgct cggcggcgga caggatggaa cgacgtggca tatttactga tccgttctca
     2341 agtattgata cagggtttcg cgactgattc cgaattcacg agcaagcttg gtcttttgct
     2401 cgccagcctc gacacgttgg cgcagttcgg caatacgctc agacgacagg gatttcttcc
     2461 tgccacggta agccccgcgt tgcttggcga gcgcaatacc ctcgcgctga cgctcgcgga
     2521 tcagggcgcg ctcgaactcg gcgaacgcgc ccatcaccga gagcatcagg ttcgccatcg
     2581 gagagtcttc gccagtaaaa ctgaggtgtt ccttgacgaa ttcgatatgc acgccgcgtt
     2641 gtgtcagcgt ttgcacgatc cggcgcaaat catcgagatt gcgcgccagg cgatccatgc
     2701 tatgcaccac cacggtgtcg ccggtgcggg cgaagcttat cagcgcttcc agttgcggac
     2761 gcttgacatc cttgccggat gccttgtcgc taaaagcgcg atcaaccttg acgccttcca
     2821 gttgccgttc cgggttctgg tcgaaggtgc tgaccctgat atacccaatg cgctgtccag
     2881 tcatggaatt ccctgcaaaa tgtcagggaa gactctatga ccttcaacga gatatgtcaa
     2941 taaattcaaa attcaatcct atcctgacgc aatttacaca tggcatctga catcaggtta
     3001 gggtatgcct caacctgacg gcggcgaatc acaagcgtcc ggtttgacgc tggtttcggt
     3061 cgcagtgctg gcccgcgcct ggaagcgctg atagcactcc agcccgcaga aatgctccac
     3121 gtactcggcc ccttccggcg tgaaggcggc atcgagcggg atttccttgc agcacacgca
     3181 gcaactggtg ctcggttcat tggcgttcat ggtggtgttc ctccattggt tgacgaagcc
     3241 gacgaaggcc gccgccggca tcggcctggc gaacaggaaa ccctgcaccg tgtcgcaacc
     3301 cgcctgccgc aaccacgcaa ggcagtcggg tgtttccaca ccctcggcta ccacctccat
     3361 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
     3421 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
     3481 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
     3541 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
     3601 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
     3661 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
     3721 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
     3781 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
     3841 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
     3901 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
     3961 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
     4021 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
     4081 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
     4141 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
     4201 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
     4261 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
     4321 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     4381 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     4441 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     4501 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     4561 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     4621 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     4681 atgttacgca gcagggcagt cgccctaaaa caaagttggg cgaacccgga gcctcattaa
     4741 ttgttagccg ttaaaattaa gccctttacc aaaccaatac ttattatgaa aaacacaata
     4801 catatcaact tcgctatttt tttaataatt gcaaatatta tctacagcag cgccagtgca
     4861 tcaacagata tctctactgt tgcatctcca ttatttgaag gaactgaagg ttgtttttta
     4921 ctttacgatg catccacaaa cgctgaaatt gctcaattca ataaagcaaa gtgtgcaacg
     4981 caaatggcac cagattcaac tttcaagatc gcattatcac ttatggcatt tgatgcggaa
     5041 ataatagatc agaaaaccat attcaaatgg gataaaaccc ccaaaggaat ggagatctgg
     5101 aacagcaatc atacaccaaa gacgtggatg caattttctg ttgtttgggt ttcgcaagaa
     5161 ataacccaaa aaattggatt aaataaaatc aagaattatc tcaaagattt tgattatgga
     5221 aatcaagact tctctggaga taaagaaaga aacaacggat taacagaagc atggctcgaa
     5281 agtagcttaa aaatttcacc agaagaacaa attcaattcc tgcgtaaaat tattaatcac
     5341 aatctcccag ttaaaaactc agccatagaa aacaccatag agaacatgta tctacaagat
     5401 ctggataata gtacaaaact gtatgggaaa actggtgcag gattcacagc aaatagaacc
     5461 ttacaaaacg gatggtttga agggtttatt ataagcaaat caggacataa atatgttttt
     5521 gtgtccgcac ttacaggaaa cttggggtcg aatttaacat caagcataaa agccaagaaa
     5581 aatgcgatca ccattctaaa cacactaaat ttataaaaaa tctaatggca aaatcgccca
     5641 acccttcaat caagtcggga cggccaaaag caagcttttg gctcccctcg ctggcgctcg
     5701 gcgcccctta tttcaaacgt taaacatcat gagggaagtg gtgatcgccg aagtatcgac
     5761 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     5821 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     5881 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     5941 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     6001 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     6061 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     6121 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     6181 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     6241 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     6301 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     6361 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     6421 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca
     6481 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag
     6541 ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc
     6601 ggtgatgctg ccaacttact gatttagtgt atgatgg
//
LOCUS       DAJTNA010000488         5963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.guided.218, whole genome shotgun sequence.
ACCESSION   DAJTNA010000488 DAJTNA010000000
VERSION     DAJTNA010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.guided.218"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            <1..652
                     /locus_tag="OO161_004509"
     CDS             <1..652
                     /locus_tag="OO161_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5465261.1"
                     /translation="HPIIRGTNKVTGQSILQRHIASFSHHGLPQGSDDALRYIAEHDD
                     ALAFACINRQMIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNG
                     LVIIPGSRGDYSWIVQPVANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTEL
                     GSRIICRDKQLIFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKSSGGKKG"
     gene            649..1263
                     /locus_tag="OO161_004510"
     CDS             649..1263
                     /locus_tag="OO161_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR5465262.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OO161_004511"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OO161_004511"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5465263.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEISAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="OO161_004512"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="OO161_004512"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5465264.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="OO161_004513"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="OO161_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5465265.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="OO161_004514"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="OO161_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5465266.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPALEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..>5963)
                     /locus_tag="OO161_004515"
     CDS             complement(5330..>5963)
                     /locus_tag="OO161_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5465267.1"
                     /translation="NFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQN
                     GDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIY
                     LATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGGED
                     LVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVAIGMTYQF"
BASE COUNT         1446 a         1449 c         1574 g         1494 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttc tcgcatcatg gtttgcctca aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgtg tattaatcgc cagatgatcg ctttgcgcat
      181 aatgcaacaa gttaaggcca cgggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcacg tggtgattac tcctggattg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggca gggcgtaaat gggggcgcac
      421 cgagtgtaaa tggcgtctgg cagcgaaata cacggtgacg caactctcgc ggactgaact
      481 tggcagtcgg ataatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggcc ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaag cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggac
      721 aggctaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg tggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggag
      961 caatccgatg cgatccggta tgagacgctg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg aaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgtcttgtt gaacaggcgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcagt
     1681 gaacggatca ggcagtgaat ttctacgtta ttgtcagtca tggtcaccac accgacgttc
     1741 agggaggttt caaccacacc tttggctaca tcggagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc agtgcgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcaggt ccagttcttc tgcatgaccc gccaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttcaccgccg gagtgaccgc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccaa aagcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc ggaccgtgaa ccacgttttc gtcagccaga
     2401 accgccagtg cagaggccat accaatgccg ttatccgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccgg tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgagagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tagttattga
     3301 tgacctggtg gataccggtg gtactgctgt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tattccgcaa gatacctgga tcgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcaggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgtt ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc tcttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggtatt gacccacgtg
     3781 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc tgaacgtacc gacgatgatt
     3841 tatacgatac tgtgattggc taccgtggtg gcaactggat ttatgagtgg gccacccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgacact
     3961 ggctgcatgc ggctacgttg tacaacattg ccgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttatcaaacc gcgcctatga agaggccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgtga
     4261 tgctgactat tgatatgccg tcggtgggct tttcttcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc tgcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctggga cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaattttgc caatttggta
     4861 aaacagttgc atcacaacag gagatagcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ctggaagtgc
     5041 gtgaattctg gggctggtgg atggagctgg aagcgcagga atcccgtttt acctacagct
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagatactg
     5161 aagtggttga acgactggag cacaccctgc gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgacgag ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgca tcacatccgg caatatctat taaaactgat
     5341 acgtcatccc aatcgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaatgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattaacc agatcttcac caccgatgcc ctcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgagtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttg
//
LOCUS       DAJTNA010000489         5962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.guided.219, whole genome shotgun sequence.
ACCESSION   DAJTNA010000489 DAJTNA010000000
VERSION     DAJTNA010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.guided.219"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO161_004516"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO161_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5465268.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2399
                     /locus_tag="OO161_004517"
                     /note="aph(3'')-Ib"
     CDS             1596..2399
                     /locus_tag="OO161_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5465269.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2399..3235
                     /locus_tag="OO161_004518"
     CDS             2399..3235
                     /locus_tag="OO161_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5465270.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSSAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3342..3746)
                     /locus_tag="OO161_004519"
                     /pseudo
     CDS             complement(3342..3746)
                     /locus_tag="OO161_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3790..4179)
                     /locus_tag="OO161_004520"
     CDS             complement(3790..4179)
                     /locus_tag="OO161_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465271.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4397..4837
                     /locus_tag="OO161_004521"
     CDS             4397..4837
                     /locus_tag="OO161_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5465272.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5582..5731)
                     /locus_tag="OO161_004522"
     CDS             complement(5582..5731)
                     /locus_tag="OO161_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5465273.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1451 a         1571 c         1551 g         1389 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     2161 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     2221 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     2281 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     2341 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     2401 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     2461 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     2521 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     2581 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     2641 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     2701 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     2761 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     2821 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     2881 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     2941 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
     3001 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     3061 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     3121 ccaggcgtac gcttatgggt gcctttcctc agcttggaac gcggatggag aagaggagca
     3181 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc
     3241 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
     3301 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
     3361 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
     3421 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
     3481 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
     3541 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
     3601 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     3661 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     3721 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     3781 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     3841 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     3901 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     3961 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     4021 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     4081 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     4141 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     4201 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     4261 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     4321 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     4381 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     4441 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     4501 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     4561 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     4621 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     4681 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     4741 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     4801 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     4861 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     4921 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4981 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     5041 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     5101 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     5161 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     5221 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     5281 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     5341 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     5401 atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa
     5461 aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat
     5521 gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa
     5581 gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt
     5641 cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg
     5701 aatccagttg ttcatcatat tcagatggca tggggtgata tccagtagct gagaggttgt
     5761 tagcgggttt cggggcgcag ccctgaacca gtcatgtagc gctagcggag tgtatactgg
     5821 cttactatgt tgccacgatt gcgaaaatca gtgaagtgct tcatgtggca ggagaaaaaa
     5881 ggcagcaccg gcgcgtcagc agaatatgcg atacaggaga tattccgctt cctcgctcac
     5941 tgactcgcta cgctcggtcg tt
//
LOCUS       DAJTNA010000490         4422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.guided.263, whole genome shotgun sequence.
ACCESSION   DAJTNA010000490 DAJTNA010000000
VERSION     DAJTNA010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.guided.263"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            67..282
                     /locus_tag="OO161_004523"
     CDS             67..282
                     /locus_tag="OO161_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5465274.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            275..661
                     /locus_tag="OO161_004524"
     CDS             275..661
                     /locus_tag="OO161_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5465275.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            669..1355
                     /locus_tag="OO161_004525"
     CDS             669..1355
                     /locus_tag="OO161_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5465276.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1333..1956)
                     /gene="tetR(B)"
                     /locus_tag="OO161_004526"
     CDS             complement(1333..1956)
                     /gene="tetR(B)"
                     /locus_tag="OO161_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5465277.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2038..3243
                     /gene="tet(B)"
                     /locus_tag="OO161_004527"
     CDS             2038..3243
                     /gene="tet(B)"
                     /locus_tag="OO161_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5465278.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3356..3949)
                     /gene="tetC"
                     /locus_tag="OO161_004528"
     CDS             complement(3356..3949)
                     /gene="tetC"
                     /locus_tag="OO161_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5465279.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4037..4399
                     /locus_tag="OO161_004529"
     CDS             4037..4399
                     /locus_tag="OO161_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5465280.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     YQFDSQQSFTRRFKGFVE"
BASE COUNT         1254 a          788 c          933 g         1447 t
ORIGIN      
        1 gaacataaaa cactatcaat aagttggagt cattaccgac cactattaag tcatgtagct
       61 ggttttattt caattgaacg ttttcaaagt ctagctacag aaggaaaaat gttatcgcta
      121 tcttggtggg aaaacgaata cgcagttctg caatggaaaa atcatgtttt acatgcgaaa
      181 gctcaacaag aagggcgaga gtcaatattt gatttttaca aaattagtat tgctcatatt
      241 actcgcgaat attcatttaa aaaggacaag gataatgttt gatgttcacg ttgttttaga
      301 taatcaaata ggacaattag cattactagg aaaaacatta ggtaataaag gtattggatt
      361 ggaaggggga gggatattta cggttggtga tgaatgccat gctcattttc ttgttgaaca
      421 aggaaaggaa gctaaaatag cgctagagca agctggactg ttagtacttg cgatccggac
      481 accattaatt cgtaagttaa aacaggaaaa accgggggaa cttggcgaaa tagcacgagt
      541 attggcggag aataacatta atattttagt gcaatacagt gaccatgcta accaactgat
      601 attaataacg gacaatgata gtatggctgc atctgttacg ctcccttggg caataaagtg
      661 aacttgcgat ggctaattta atacgaaaag aggttacctt tgagtcctca atagccgcga
      721 taggggcggc tatgtctgac atttcacgag ttaaaatact cagtgctttg atggatgggc
      781 gagcttggac ggccactgag ctaagttctg tggcgaatat atcagcttca acggcgagca
      841 gtcatttatc taaattatta gattgccagc taatcacagt agtagctcaa ggcaagcatc
      901 gttattttcg gctagcagga aaagatattg ctgaattgat ggaaagtatg atggggatct
      961 ccttaaacca tggcgtacat gccaaagttt ccacgccagt gcatttacga aaagcacgta
     1021 cttgctatga tcatttagct ggcgaagttg ccgttaagat ctatgattcc ctttgtcaac
     1081 agcaatggat cactgaaaat ggttcaatga tcacattaag tggtattcaa tattttcatg
     1141 aaatgggaat tgacgttcct tccaaacatt cacgtaaaat ctgttgtgcg tgtttagatt
     1201 ggagtgaacg ccgtttccat ttaggtgggt acgttggagc cgcattattt tcgctttatg
     1261 aatctaaagg gtggttaact cgacatcttg gttaccgtga agttaccatc acggaaaaag
     1321 gttatgctgc ttttaagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat
     1381 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
     1441 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
     1501 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
     1561 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
     1621 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
     1681 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
     1741 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
     1801 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
     1861 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
     1921 gctgttaatc actttacttt tatctaatct agacatcatt aattcctaat ttttgttgac
     1981 actctatcat tgatagagtt attttaccac tccctatcag tgatagagaa aagtgaaatg
     2041 aatagttcga caaagatcgc attggtaatt acgttactcg atgccatggg gattggcctt
     2101 atcatgccag tcttgccaac gttattacgt gaatttattg cttcggaaga tatcgctaac
     2161 cactttggcg tattgcttgc actttatgcg ttaatgcagg ttatctttgc tccttggctt
     2221 ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt tgttgtcatt aataggcgca
     2281 tcgctggatt acttattgct ggctttttca agtgcgcttt ggatgctgta tttaggccgt
     2341 ttgctttcag ggatcacagg agctactggg gctgtcgcgg catcggtcat tgccgatacc
     2401 acctcagctt ctcaacgcgt gaagtggttc ggttggttag gggcaagttt tgggcttggt
     2461 ttaatagcgg ggcctattat tggtggtttt gcaggagaga tttcaccgca tagtcccttt
     2521 tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg ttatgttttg gttccgtgaa
     2581 accaaaaata cacgtgataa tacagatacc gaagtagggg ttgagacgca atcgaattcg
     2641 gtatacatca ctttatttaa aacgatgccc attttgttga ttatttattt ttcagcgcaa
     2701 ttgataggcc aaattcccgc aacggtgtgg gtgctattta ccgaaaatcg ttttggatgg
     2761 aatagcatga tggttggctt ttcattagcg ggtcttggtc ttttacactc agtattccaa
     2821 gcctttgtgg caggaagaat agccactaaa tggggcgaaa aaacggcagt actgctcgga
     2881 tttattgcag atagtagtgc atttgccttt ttagcgttta tatctgaagg ttggttagtt
     2941 ttccctgttt taattttatt ggctggtggt gggatcgctt tacctgcatt acagggagtg
     3001 atgtctatcc aaacaaagag tcatcagcaa ggtgctttac agggattatt ggtgagcctt
     3061 accaatgcaa ccggtgttat tggcccatta ctgtttgctg ttatttataa tcattcacta
     3121 ccaatttggg atggctggat ttggattatt ggtttagcgt tttactgtat tattatcctg
     3181 ctatcgatga ccttcatgtt aacccctcaa gctcagggga gtaaacagga gacaagtgct
     3241 tagttatttc gtcaccaaat gatgttattc cgcgaaatat aatgaccctc ttgataaccc
     3301 aagagggcat tttttacgat aaagaagatt tagcttcaaa taaaacctat ctattttatt
     3361 tatctttcaa gctcaataaa aagccgcggt aaatagcaat aaattggcct tttttatcgg
     3421 caagctcttt taggtttttc gcatgtattg cgatatgcat aaaccagcca ttgagtaagt
     3481 ttttaagcac atcatcatca taagctttaa gttggttctc ttggatcaat ttgctgacaa
     3541 tggcgtttac cttaccagta atgtattcaa ggctaatttt ttcaagttca ttccaaccaa
     3601 tgataggcat cacttcttgg atagggataa ggtttttatt attatcaata atataatcaa
     3661 gataatgttc aaatatactt tctaaggcag accaaccatt tgttaaatca gtttttgttg
     3721 tgatgtaggc atcaatcata attaattgct gcttataaca ggcactgagt aattgttttt
     3781 tatttttaaa gtgatgataa aaggcacctt tggtcaccaa cgcttttccc gagatctcat
     3841 ctattgaaac agcttgatag cctttttcaa caaacaatat tcgtgctgag ttaaccagtg
     3901 attgataggt actcttaaaa ttttcttgtt gatgattttt attttccatg atagatttaa
     3961 aataacatac cgtcagtatg tttatggtat catgatgatg tggtcgtgac aatcttaaga
     4021 acatttaggt tattttatgt atattgaaca gcattctcgc tatcaaaata aagctaataa
     4081 catccaatta agatatgatg ataagcagtt tcatacaacg gttatcaaag atgttctatt
     4141 atggattgaa cataatttag atcagtcttt actgcttgat gatgtggcga ataaagcggg
     4201 ttataccaag tggtattttc agcggctgtt caaaaaagta acaggggtca cactggctag
     4261 ctatattcgt gctcgtcgtt tgacgaaagc ggctgttgag ttgaggttga cgaaaaaaac
     4321 tatccttgag atcgcattaa aatatcaatt tgattcccaa caatctttta cacgtcgatt
     4381 taagggattt gttgaataaa tcgaactttt gctgagttga ag
//
LOCUS       DAJTNA010000491         2208 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 603782
            SAMEA8900754-rid15819963.guided.337, whole genome shotgun sequence.
ACCESSION   DAJTNA010000491 DAJTNA010000000
VERSION     DAJTNA010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900754
            Sequence Read Archive: ERR6005893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478807.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:22:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 198 of 386
            Pseudo Genes (incomplete)         :: 105 of 386
            Pseudo Genes (internal stop)      :: 127 of 386
            Pseudo Genes (multiple problems)  :: 42 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900754-rid15819963.guided.337"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="603782"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Bangladesh"
                     /collection_date="2010-05-03"
     gene            260..919
                     /locus_tag="OO161_004530"
     CDS             260..919
                     /locus_tag="OO161_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5465281.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1120..1497)
                     /locus_tag="OO161_004531"
     CDS             complement(1120..1497)
                     /locus_tag="OO161_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5465282.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1564..>2208)
                     /locus_tag="OO161_004532"
     CDS             complement(1564..>2208)
                     /locus_tag="OO161_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR5465283.1"
                     /translation="RIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILR
                     LATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVH
                     AGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAA
                     HALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          529 a          554 c          583 g          542 t
ORIGIN      
        1 catcatacac taaatcagta agttggcagc atcacccgac gcactttgcg ccgaataaat
       61 acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg
      121 gaagccctgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa
      181 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      241 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      301 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      361 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      421 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
      481 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      541 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      601 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
      661 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
      721 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
      781 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
      841 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
      901 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg
      961 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac
     1021 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc
     1081 ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt
     1141 gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag
     1201 agcctgataa aaacggttag cgccggggtt ggatttttca gcgttccagc taaggctaag
     1261 gcattcctgt tcaagcgcaa gccgggctat aaagcgcatt atcgctttac ccgtgccctt
     1321 atttcgatcg cactgagaaa catacagctc tttaatatgc agctgaccgc tgtatcgggg
     1381 cgagggataa agaatattgc aacatgccag gccagtaatg ttattgccgc agcgcgctct
     1441 gatcaccagg gtaccggaaa gccggttgaa taacttttta cagagataat ccttcatcaa
     1501 cgcttcctga atgatacctt cgccataata ggggtcgtct cagaaaacgg aaaataaagc
     1561 acgctaagcc ggttgcagag gccgtagcgg cctgaacttc cccgcgccga tcttggcgct
     1621 gctgcgccat aggtaatcac cggtcaggtt gatgtgctcc cagccgagtg gcgacaggta
     1681 ctgcaatagc gagtcatcga cggcatgacc attgccgcgc aacgcatgcg ccgcacgctc
     1741 caggtagacc gtgttccaca gcacgatggc cgccgtcacc aggttgaggc cgctggcccg
     1801 gtagcgctgc tgctcgaaac tgcggtcacg gatttcacca aggcggttga agaacacggc
     1861 acgggccagc gcattgcgcg cctcgccctt gttcagcccg gcatgcacgc ggcggcgtag
     1921 ctcgacgctt tgcagccagt cgaggatgaa cagcgtgcgc tcgatgcggc ccaactcgcg
     1981 cagcgcgacg gccaagccgt tctggcgcgg gtagctgccg agtttcctga gcatcagcga
     2041 ggccgtcacc gtgccctgct tgatcgaggt ggccagccgc aggatttcgt cccaatgggc
     2101 gcggacgtgc ttgatgttga gcgtgccgcc gatcatcggc ttgagcgcgt cataggcggc
     2161 atcgcccttc gggatgtaga gcttggtgtc gcccaggtcg cggatgcg
//
